CACNA1E gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001205293.1	NP_001192222.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform 1
XM_017002250.1	XP_016857739.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X8
NM_001205294.2	NP_001192223.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform 2
NM_001205294.1	NP_001192223.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform 2
XM_017002247.1	XP_016857736.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
NM_001205293.3	NP_001192222.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform 1
NM_000721.4	NP_000712.2	voltage-dependent R-type calcium channel subunit alpha-1E isoform 3
XM_017002244.1	XP_016857733.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
XM_017002248.1	XP_016857737.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
XM_017002249.1	XP_016857738.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X7
XM_017002251.1	XP_016857740.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
NM_000721.3	NP_000712.2	voltage-dependent R-type calcium channel subunit alpha-1E isoform 3
XM_017002243.1	XP_016857732.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
XM_017002245.1	XP_016857734.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
XM_017002246.1	XP_016857735.1	voltage-dependent R-type calcium channel subunit alpha-1E isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10572	NM_000721.4 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu10868	NM_001205294.2 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu11422	NM_001205293.3 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu90473	XM_017002243.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu90473	XM_017002251.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu90474	XM_017002244.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu90475	XM_017002245.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu90476	XM_017002246.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu90477	XM_017002247.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu90478	XM_017002248.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu90479	XM_017002249.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu90480	XM_017002250.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
View Old Accession Versions >>
OHu10868	NM_001205294.1	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu11422	NM_001205293.1	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu10572	NM_000721.3	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
Hide Old Accession Versions >>

CACNA1E ( NM_000721.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000712.2 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu10572
Clone ID Related Accession (Same CDS sequence)	NM_000721.4 , NM_000721.3
Accession Version	NM_000721.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160059.7, BP230397.1, L29385.1, AK309070.1, AB209499.1, AL161734.12, BC037800.1 and KF510705.1. On May 31, 2019 this sequence version replaced NM_000721.3. Transcript Variant: This variant (3) is missing an in-frame coding exon compared to variant 1. This results in a shorter isoform (3, also known as alpha-1E-3) missing an internal protein segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L29385.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG 
CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT 
GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG 
TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC 
AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC 
GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT 
CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG 
GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG 
ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG 
AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA 
AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA 
GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA 
CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC 
AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG 
ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC 
TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC 
AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC 
AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG 
AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA 
GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT 
GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC 
ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT 
ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA 
GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCGATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCCGT GTGGGAGCAG 
CGTACCAGCC AGCTGAGGAA GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAGAGAG 
GAGGCGCCGA CCATGAACCC GCTCAACCCC CTCAACCCGC TCAGCTCCCT CAACCCGCTC 
AATGCCCACC CCAGCCTTTA TCGGCGACCC AGGGCCATTG AGGGCCTGGC CCTGGGCCTG 
GCCCTGGAGA AGTTCGAGGA GGAGCGCATC AGCCGTGGGG GGTCCCTCAA GGGGGATGGA 
GGGGACCGAT CCAGTGCCCT GGACAACCAG AGGACCCCTT TGTCCCTGGG CCAGCGGGAG 
CCACCATGGC TGGCCAGGCC CTGTCATGGA AACTGTGACC CGACTCAGCA GGAGGCAGGG 
GGAGGAGAGG CTGTGGTGAC CTTTGAGGAC CGGGCCAGGC ACAGGCAGAG CCAACGGCGC 
AGCCGGCATC GCCGCGTCAG GACAGAAGGC AAGGAGTCCT CTTCAGCCTC CCGGAGCAGG 
TCTGCCAGCC AGGAACGCAG TCTGGATGAA GCCATGCCCA CTGAAGGGGA GAAGGACCAT 
GAGCTCAGGG GCAACCATGG TGCCAAGGAG CCAACGATCC AAGAAGAGAG AGCCCAGGAT 
TTAAGGAGGA CCAACAGTCT GATGGTGTCC AGAGGCTCCG GGCTGGCAGG AGGCCTTGAT 
GAGGCTGACA CCCCCCTAGT CCTGCCCCAT CCTGAGCTGG AAGTGGGGAA GCACGTGGTG 
CTGACGGAGC AGGAGCCAGA AGGCAGCAGT GAGCAGGCCC TGCTGGGGAA TGTGCAGCTA 
GACATGGGCC GGGTCATCAG CCAGAGCGAG CCTGACCTCT CCTGCATCAC GGCCAACACG 
GACAAGGCCA CCACCGAGAG CACCAGCGTC ACCGTCGCCA TCCCCGACGT GGACCCCTTG 
GTGGACTCAA CCGTGGTGCA CATTAGCAAC AAGACGGATG GGGAAGCCAG TCCCTTGAAG 
GAGGCAGAGA TCAGAGAGGA TGAGGAGGAG GTGGAGAAGA AGAAGCAGAA GAAGGAGAAG 
CGTGAGACAG GCAAAGCCAT GGTGCCCCAC AGCTCAATGT TCATCTTCAG CACCACCAAC 
CCGATCCGGA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATCCTC 
CTGGTGATTG CAGCCAGCAG CATCGCCCTG GCGGCAGAGG ACCCCGTCCT GACCAACTCG 
GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTGTTCA CGGGCGTGTT CACCTTTGAG 
ATGGTTATAA AGATGATAGA CCAAGGCTTG ATCCTGCAGG ATGGGTCCTA CTTCCGAGAC 
TTGTGGAACA TCCTGGACTT TGTGGTGGTC GTTGGCGCAT TGGTGGCCTT TGCTCTGGCG 
AACGCTTTGG GAACCAACAA AGGACGGGAC ATCAAGACCA TCAAGTCTCT GCGGGTGCTC 
CGAGTTCTAA GGCCACTGAA AACCATCAAG CGCTTGCCCA AGCTCAAGGC CGTCTTCGAC 
TGCGTAGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TTGTGTACAA GCTCTTCATG 
TTCATCTTTG CTGTCATCGC AGTTCAGCTC TTCAAGGGAA AGTTCTTTTA TTGCACGGAC 
AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTATG TAGATCATGA GAAAAACAAG 
ATGGAGGTGA AGGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG 
GCCCTGCTGA CCCTCTTCAC CGTCTCCACA GGGGAAGGAT GGCCTCAAGT TCTGCAGCAC 
TCTGTAGATG TGACAGAGGA AGACCGAGGC CCAAGCCGCA GCAACCGCAT GGAGATGTCT 
ATCTTTTATG TAGTCTACTT TGTGGTCTTC CCCTTCTTCT TTGTCAATAT CTTTGTGGCT 
CTCATCATCA TCACCTTCCA GGAGCAAGGG GATAAGATGA TGGAGGAGTG CAGCCTGGAG 
AAGAATGAGA GGGCGTGCAT CGACTTCGCC ATCAGCGCCA AACCTCTCAC CCGCTACATG 
CCGCAGAACA GACACACCTT CCAGTACCGC GTGTGGCACT TTGTGGTGTC TCCGTCCTTT 
GAGTACACCA TTATGGCCAT GATCGCCTTG AATACTGTTG TGCTGATGAT GAAGTATTAT 
TCTGCTCCCT GTACCTATGA GCTGGCCCTG AAGTACCTGA ATATCGCCTT CACCATGGTG 
TTTTCCCTGG AATGTGTCCT GAAGGTCATC GCTTTTGGCT TTTTGAACTA TTTCCGAGAC 
ACCTGGAATA TCTTTGACTT CATCACCGTG ATTGGCAGTA TCACAGAAAT TATCCTGACA 
GACAGCAAGC TGGTGAACAC CAGTGGCTTC AATATGAGCT TTCTGAAGCT CTTCCGAGCT 
GCCCGCCTCA TAAAGCTCCT GCGTCAGGGC TATACCATAC GCATTTTGCT GTGGACCTTT 
GTGCAGTCCT TTAAGGCCCT CCCTTATGTC TGCCTTTTAA TTGCCATGCT TTTCTTCATT 
TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGACGAGGA GAGTCACATC 
AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTAA TGCTACTCTT CAGGAGTGCC 
ACAGGTGAGG CCTGGCAGGA GATTATGCTG TCATGCCTTG GGGAGAAGGG CTGTGAGCCT 
GACACCACCG CACCATCAGG GCAGAACGAG AACGAACGCT GCGGCACCGA TCTGGCCTAC 
GTGTACTTTG TCTCCTTCAT CTTCTTCTGC TCCTTCTTGA TGCTCAACCT GTTTGTGGCC 
GTCATCATGG ACAACTTTGA GTACCTGACT CGGGACTCCT CCATCCTGGG GCCTCACCAC 
TTGGACGAGT TTGTCCGCGT CTGGGCAGAA TATGACCGAG CAGCATGTGG CCGCATCCAT 
TACACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCTC CGCTAGGCCT CGGCAAGAGA 
TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTGATGA ACATGCCAGT AGCTGAGGAC 
ATGACGGTCC ACTTCACCTC CACACTTATG GCTCTGATCC GGACAGCTCT GGACATTAAA 
ATTGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCAG AGCTACAAAA GGAGACCCTA 
GCCATCTGGC CTCACCTATC CCAGAAGATG CTGGATCTGC TTGTGCCCAT GCCCAAAGCC 
TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAGCAG 
AGTAAGGTGA AGAAGCAGAG GCAGCAGCTG GAGGAACAGA AAAATGCCCC CATGTTCCAG 
CGCATGGAGC CTTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCTTAC 
CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGGAGTG GATACCCTTC GATGAGTCCA 
CTCTCTCCCC AGGATATATT CCAGTTGGCT TGTATGGACC CCGCCGATGA CGGACAGTTC 
CAAGAACGGC AGTCTCTGGT GGTGACAGAC CCTAGCTCCA TGAGACGTTC ATTTTCCACT 
ATTCGGGATA AGCGTTCAAA TTCCTCGTGG TTGGAGGAAT TCTCCATGGA GCGAAGCAGT 
GAAAATACCT ACAAGTCCCG TCGCCGGAGT TACCACTCCT CCTTGCGGCT GTCAGCCCAC 
CGCCTGAACT CTGATTCAGG CCACAAGTCT GACACTCACC GCTCAGGGGG CAGGGAGCGG 
GGACGATCAA AAGAGCGAAA GCATCTTCTC TCTCCTGATG TCTCCCGCTG CAATTCAGAA 
GAGCGAGGGA CCCAGGCTGA CTGGGAGTCC CCAGAGCGCC GTCAATCCAG GTCACCCAGT 
GAGGGCAGGT CACAGACGCC CAACAGACAG GGCACAGGTT CCCTAAGTGA GAGCTCCATC 
CCCTCTGTCT CTGACACCAG CACCCCAAGA AGAAGTCGTC GGCAGCTCCC ACCCGTCCCG 
CCAAAGCCCC GGCCCCTCCT TTCCTACAGC TCCCTGATTC GACACGCGGG CAGCATCTCT 
CCACCTGCTG ATGGAAGCGA GGAGGGCTCC CCGCTGACCT CCCAAGCTCT GGAGAGCAAC 
AATGCTTGCC TGACCGAGTC TTCCAACTCT CCGCACCCCC AGCAGAGCCA ACATGCCTCC 
CCACAGCGCT ACATCTCCGA GCCCTACTTG GCCCTGCACG AAGACTCCCA CGCCTCAGAC 
TGTGGTGAGG AGGAGACGCT CACTTTCGAA GCAGCCGTGG CTACTAGCCT GGGCCGTTCC 
AACACCATCG GCTCAGCCCC ACCCCTGCGG CATAGCTGGC AGATGCCCAA CGGGCACTAT 
CGGCGGCGGA GGCGCGGGGG GCCTGGGCCA GGCATGATGT GTGGGGCTGT CAACAACCTG 
CTAAGTGACA CGGAAGAAGA TGACAAATGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000712.2
CDS	229..7041
Translation	MARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFEDRARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTSVTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNNLLSDTEEDDKC

Target ORF information:

RefSeq Version	NM_000721.4
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000721.4

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG 
CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT 
GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG 
TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC 
AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC 
GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT 
CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG 
GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG 
ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG 
AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA 
AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA 
GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA 
CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC 
AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG 
ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC 
TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC 
AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC 
AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG 
AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA 
GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT 
GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC 
ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT 
ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA 
GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCGATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCCGT GTGGGAGCAG 
CGTACCAGCC AGCTGAGGAA GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAGAGAG 
GAGGCGCCGA CCATGAACCC GCTCAACCCC CTCAACCCGC TCAGCTCCCT CAACCCGCTC 
AATGCCCACC CCAGCCTTTA TCGGCGACCC AGGGCCATTG AGGGCCTGGC CCTGGGCCTG 
GCCCTGGAGA AGTTCGAGGA GGAGCGCATC AGCCGTGGGG GGTCCCTCAA GGGGGATGGA 
GGGGACCGAT CCAGTGCCCT GGACAACCAG AGGACCCCTT TGTCCCTGGG CCAGCGGGAG 
CCACCATGGC TGGCCAGGCC CTGTCATGGA AACTGTGACC CGACTCAGCA GGAGGCAGGG 
GGAGGAGAGG CTGTGGTGAC CTTTGAGGAC CGGGCCAGGC ACAGGCAGAG CCAACGGCGC 
AGCCGGCATC GCCGCGTCAG GACAGAAGGC AAGGAGTCCT CTTCAGCCTC CCGGAGCAGG 
TCTGCCAGCC AGGAACGCAG TCTGGATGAA GCCATGCCCA CTGAAGGGGA GAAGGACCAT 
GAGCTCAGGG GCAACCATGG TGCCAAGGAG CCAACGATCC AAGAAGAGAG AGCCCAGGAT 
TTAAGGAGGA CCAACAGTCT GATGGTGTCC AGAGGCTCCG GGCTGGCAGG AGGCCTTGAT 
GAGGCTGACA CCCCCCTAGT CCTGCCCCAT CCTGAGCTGG AAGTGGGGAA GCACGTGGTG 
CTGACGGAGC AGGAGCCAGA AGGCAGCAGT GAGCAGGCCC TGCTGGGGAA TGTGCAGCTA 
GACATGGGCC GGGTCATCAG CCAGAGCGAG CCTGACCTCT CCTGCATCAC GGCCAACACG 
GACAAGGCCA CCACCGAGAG CACCAGCGTC ACCGTCGCCA TCCCCGACGT GGACCCCTTG 
GTGGACTCAA CCGTGGTGCA CATTAGCAAC AAGACGGATG GGGAAGCCAG TCCCTTGAAG 
GAGGCAGAGA TCAGAGAGGA TGAGGAGGAG GTGGAGAAGA AGAAGCAGAA GAAGGAGAAG 
CGTGAGACAG GCAAAGCCAT GGTGCCCCAC AGCTCAATGT TCATCTTCAG CACCACCAAC 
CCGATCCGGA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATCCTC 
CTGGTGATTG CAGCCAGCAG CATCGCCCTG GCGGCAGAGG ACCCCGTCCT GACCAACTCG 
GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTGTTCA CGGGCGTGTT CACCTTTGAG 
ATGGTTATAA AGATGATAGA CCAAGGCTTG ATCCTGCAGG ATGGGTCCTA CTTCCGAGAC 
TTGTGGAACA TCCTGGACTT TGTGGTGGTC GTTGGCGCAT TGGTGGCCTT TGCTCTGGCG 
AACGCTTTGG GAACCAACAA AGGACGGGAC ATCAAGACCA TCAAGTCTCT GCGGGTGCTC 
CGAGTTCTAA GGCCACTGAA AACCATCAAG CGCTTGCCCA AGCTCAAGGC CGTCTTCGAC 
TGCGTAGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TTGTGTACAA GCTCTTCATG 
TTCATCTTTG CTGTCATCGC AGTTCAGCTC TTCAAGGGAA AGTTCTTTTA TTGCACGGAC 
AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTATG TAGATCATGA GAAAAACAAG 
ATGGAGGTGA AGGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG 
GCCCTGCTGA CCCTCTTCAC CGTCTCCACA GGGGAAGGAT GGCCTCAAGT TCTGCAGCAC 
TCTGTAGATG TGACAGAGGA AGACCGAGGC CCAAGCCGCA GCAACCGCAT GGAGATGTCT 
ATCTTTTATG TAGTCTACTT TGTGGTCTTC CCCTTCTTCT TTGTCAATAT CTTTGTGGCT 
CTCATCATCA TCACCTTCCA GGAGCAAGGG GATAAGATGA TGGAGGAGTG CAGCCTGGAG 
AAGAATGAGA GGGCGTGCAT CGACTTCGCC ATCAGCGCCA AACCTCTCAC CCGCTACATG 
CCGCAGAACA GACACACCTT CCAGTACCGC GTGTGGCACT TTGTGGTGTC TCCGTCCTTT 
GAGTACACCA TTATGGCCAT GATCGCCTTG AATACTGTTG TGCTGATGAT GAAGTATTAT 
TCTGCTCCCT GTACCTATGA GCTGGCCCTG AAGTACCTGA ATATCGCCTT CACCATGGTG 
TTTTCCCTGG AATGTGTCCT GAAGGTCATC GCTTTTGGCT TTTTGAACTA TTTCCGAGAC 
ACCTGGAATA TCTTTGACTT CATCACCGTG ATTGGCAGTA TCACAGAAAT TATCCTGACA 
GACAGCAAGC TGGTGAACAC CAGTGGCTTC AATATGAGCT TTCTGAAGCT CTTCCGAGCT 
GCCCGCCTCA TAAAGCTCCT GCGTCAGGGC TATACCATAC GCATTTTGCT GTGGACCTTT 
GTGCAGTCCT TTAAGGCCCT CCCTTATGTC TGCCTTTTAA TTGCCATGCT TTTCTTCATT 
TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGACGAGGA GAGTCACATC 
AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTAA TGCTACTCTT CAGGAGTGCC 
ACAGGTGAGG CCTGGCAGGA GATTATGCTG TCATGCCTTG GGGAGAAGGG CTGTGAGCCT 
GACACCACCG CACCATCAGG GCAGAACGAG AACGAACGCT GCGGCACCGA TCTGGCCTAC 
GTGTACTTTG TCTCCTTCAT CTTCTTCTGC TCCTTCTTGA TGCTCAACCT GTTTGTGGCC 
GTCATCATGG ACAACTTTGA GTACCTGACT CGGGACTCCT CCATCCTGGG GCCTCACCAC 
TTGGACGAGT TTGTCCGCGT CTGGGCAGAA TATGACCGAG CAGCATGTGG CCGCATCCAT 
TACACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCTC CGCTAGGCCT CGGCAAGAGA 
TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTGATGA ACATGCCAGT AGCTGAGGAC 
ATGACGGTCC ACTTCACCTC CACACTTATG GCTCTGATCC GGACAGCTCT GGACATTAAA 
ATTGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCAG AGCTACAAAA GGAGACCCTA 
GCCATCTGGC CTCACCTATC CCAGAAGATG CTGGATCTGC TTGTGCCCAT GCCCAAAGCC 
TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAGCAG 
AGTAAGGTGA AGAAGCAGAG GCAGCAGCTG GAGGAACAGA AAAATGCCCC CATGTTCCAG 
CGCATGGAGC CTTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCTTAC 
CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGGAGTG GATACCCTTC GATGAGTCCA 
CTCTCTCCCC AGGATATATT CCAGTTGGCT TGTATGGACC CCGCCGATGA CGGACAGTTC 
CAAGAACGGC AGTCTCTGGT GGTGACAGAC CCTAGCTCCA TGAGACGTTC ATTTTCCACT 
ATTCGGGATA AGCGTTCAAA TTCCTCGTGG TTGGAGGAAT TCTCCATGGA GCGAAGCAGT 
GAAAATACCT ACAAGTCCCG TCGCCGGAGT TACCACTCCT CCTTGCGGCT GTCAGCCCAC 
CGCCTGAACT CTGATTCAGG CCACAAGTCT GACACTCACC GCTCAGGGGG CAGGGAGCGG 
GGACGATCAA AAGAGCGAAA GCATCTTCTC TCTCCTGATG TCTCCCGCTG CAATTCAGAA 
GAGCGAGGGA CCCAGGCTGA CTGGGAGTCC CCAGAGCGCC GTCAATCCAG GTCACCCAGT 
GAGGGCAGGT CACAGACGCC CAACAGACAG GGCACAGGTT CCCTAAGTGA GAGCTCCATC 
CCCTCTGTCT CTGACACCAG CACCCCAAGA AGAAGTCGTC GGCAGCTCCC ACCCGTCCCG 
CCAAAGCCCC GGCCCCTCCT TTCCTACAGC TCCCTGATTC GACACGCGGG CAGCATCTCT 
CCACCTGCTG ATGGAAGCGA GGAGGGCTCC CCGCTGACCT CCCAAGCTCT GGAGAGCAAC 
AATGCTTGCC TGACCGAGTC TTCCAACTCT CCGCACCCCC AGCAGAGCCA ACATGCCTCC 
CCACAGCGCT ACATCTCCGA GCCCTACTTG GCCCTGCACG AAGACTCCCA CGCCTCAGAC 
TGTGGTGAGG AGGAGACGCT CACTTTCGAA GCAGCCGTGG CTACTAGCCT GGGCCGTTCC 
AACACCATCG GCTCAGCCCC ACCCCTGCGG CATAGCTGGC AGATGCCCAA CGGGCACTAT 
CGGCGGCGGA GGCGCGGGGG GCCTGGGCCA GGCATGATGT GTGGGGCTGT CAACAACCTG 
CTAAGTGACA CGGAAGAAGA TGACAAATGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( NM_000721.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000712.2 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 3, mRNA.

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CloneID	OHu10572
Clone ID Related Accession (Same CDS sequence)	NM_000721.4 , NM_000721.3
Accession Version	NM_000721.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP230397.1, L29385.1, AK309070.1, AB209499.1, AL161734.12 and BC037800.1. On Apr 20, 2011 this sequence version replaced NM_000721.2. Transcript Variant: This variant (3) is missing an in-frame coding exon compared to variant 1. This results in a shorter isoform (3, also known as alpha-1E-3) missing an internal protein segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L29385.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG 
CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT 
GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG 
TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC 
AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC 
GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT 
CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG 
GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG 
ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG 
AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA 
AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA 
GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA 
CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC 
AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG 
ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC 
TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC 
AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC 
AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG 
AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA 
GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT 
GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC 
ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT 
ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA 
GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCGATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCCGT GTGGGAGCAG 
CGTACCAGCC AGCTGAGGAA GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAGAGAG 
GAGGCGCCGA CCATGAACCC GCTCAACCCC CTCAACCCGC TCAGCTCCCT CAACCCGCTC 
AATGCCCACC CCAGCCTTTA TCGGCGACCC AGGGCCATTG AGGGCCTGGC CCTGGGCCTG 
GCCCTGGAGA AGTTCGAGGA GGAGCGCATC AGCCGTGGGG GGTCCCTCAA GGGGGATGGA 
GGGGACCGAT CCAGTGCCCT GGACAACCAG AGGACCCCTT TGTCCCTGGG CCAGCGGGAG 
CCACCATGGC TGGCCAGGCC CTGTCATGGA AACTGTGACC CGACTCAGCA GGAGGCAGGG 
GGAGGAGAGG CTGTGGTGAC CTTTGAGGAC CGGGCCAGGC ACAGGCAGAG CCAACGGCGC 
AGCCGGCATC GCCGCGTCAG GACAGAAGGC AAGGAGTCCT CTTCAGCCTC CCGGAGCAGG 
TCTGCCAGCC AGGAACGCAG TCTGGATGAA GCCATGCCCA CTGAAGGGGA GAAGGACCAT 
GAGCTCAGGG GCAACCATGG TGCCAAGGAG CCAACGATCC AAGAAGAGAG AGCCCAGGAT 
TTAAGGAGGA CCAACAGTCT GATGGTGTCC AGAGGCTCCG GGCTGGCAGG AGGCCTTGAT 
GAGGCTGACA CCCCCCTAGT CCTGCCCCAT CCTGAGCTGG AAGTGGGGAA GCACGTGGTG 
CTGACGGAGC AGGAGCCAGA AGGCAGCAGT GAGCAGGCCC TGCTGGGGAA TGTGCAGCTA 
GACATGGGCC GGGTCATCAG CCAGAGCGAG CCTGACCTCT CCTGCATCAC GGCCAACACG 
GACAAGGCCA CCACCGAGAG CACCAGCGTC ACCGTCGCCA TCCCCGACGT GGACCCCTTG 
GTGGACTCAA CCGTGGTGCA CATTAGCAAC AAGACGGATG GGGAAGCCAG TCCCTTGAAG 
GAGGCAGAGA TCAGAGAGGA TGAGGAGGAG GTGGAGAAGA AGAAGCAGAA GAAGGAGAAG 
CGTGAGACAG GCAAAGCCAT GGTGCCCCAC AGCTCAATGT TCATCTTCAG CACCACCAAC 
CCGATCCGGA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATCCTC 
CTGGTGATTG CAGCCAGCAG CATCGCCCTG GCGGCAGAGG ACCCCGTCCT GACCAACTCG 
GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTGTTCA CGGGCGTGTT CACCTTTGAG 
ATGGTTATAA AGATGATAGA CCAAGGCTTG ATCCTGCAGG ATGGGTCCTA CTTCCGAGAC 
TTGTGGAACA TCCTGGACTT TGTGGTGGTC GTTGGCGCAT TGGTGGCCTT TGCTCTGGCG 
AACGCTTTGG GAACCAACAA AGGACGGGAC ATCAAGACCA TCAAGTCTCT GCGGGTGCTC 
CGAGTTCTAA GGCCACTGAA AACCATCAAG CGCTTGCCCA AGCTCAAGGC CGTCTTCGAC 
TGCGTAGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TTGTGTACAA GCTCTTCATG 
TTCATCTTTG CTGTCATCGC AGTTCAGCTC TTCAAGGGAA AGTTCTTTTA TTGCACGGAC 
AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTATG TAGATCATGA GAAAAACAAG 
ATGGAGGTGA AGGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG 
GCCCTGCTGA CCCTCTTCAC CGTCTCCACA GGGGAAGGAT GGCCTCAAGT TCTGCAGCAC 
TCTGTAGATG TGACAGAGGA AGACCGAGGC CCAAGCCGCA GCAACCGCAT GGAGATGTCT 
ATCTTTTATG TAGTCTACTT TGTGGTCTTC CCCTTCTTCT TTGTCAATAT CTTTGTGGCT 
CTCATCATCA TCACCTTCCA GGAGCAAGGG GATAAGATGA TGGAGGAGTG CAGCCTGGAG 
AAGAATGAGA GGGCGTGCAT CGACTTCGCC ATCAGCGCCA AACCTCTCAC CCGCTACATG 
CCGCAGAACA GACACACCTT CCAGTACCGC GTGTGGCACT TTGTGGTGTC TCCGTCCTTT 
GAGTACACCA TTATGGCCAT GATCGCCTTG AATACTGTTG TGCTGATGAT GAAGTATTAT 
TCTGCTCCCT GTACCTATGA GCTGGCCCTG AAGTACCTGA ATATCGCCTT CACCATGGTG 
TTTTCCCTGG AATGTGTCCT GAAGGTCATC GCTTTTGGCT TTTTGAACTA TTTCCGAGAC 
ACCTGGAATA TCTTTGACTT CATCACCGTG ATTGGCAGTA TCACAGAAAT TATCCTGACA 
GACAGCAAGC TGGTGAACAC CAGTGGCTTC AATATGAGCT TTCTGAAGCT CTTCCGAGCT 
GCCCGCCTCA TAAAGCTCCT GCGTCAGGGC TATACCATAC GCATTTTGCT GTGGACCTTT 
GTGCAGTCCT TTAAGGCCCT CCCTTATGTC TGCCTTTTAA TTGCCATGCT TTTCTTCATT 
TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGACGAGGA GAGTCACATC 
AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTAA TGCTACTCTT CAGGAGTGCC 
ACAGGTGAGG CCTGGCAGGA GATTATGCTG TCATGCCTTG GGGAGAAGGG CTGTGAGCCT 
GACACCACCG CACCATCAGG GCAGAACGAG AACGAACGCT GCGGCACCGA TCTGGCCTAC 
GTGTACTTTG TCTCCTTCAT CTTCTTCTGC TCCTTCTTGA TGCTCAACCT GTTTGTGGCC 
GTCATCATGG ACAACTTTGA GTACCTGACT CGGGACTCCT CCATCCTGGG GCCTCACCAC 
TTGGACGAGT TTGTCCGCGT CTGGGCAGAA TATGACCGAG CAGCATGTGG CCGCATCCAT 
TACACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCTC CGCTAGGCCT CGGCAAGAGA 
TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTGATGA ACATGCCAGT AGCTGAGGAC 
ATGACGGTCC ACTTCACCTC CACACTTATG GCTCTGATCC GGACAGCTCT GGACATTAAA 
ATTGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCAG AGCTACAAAA GGAGACCCTA 
GCCATCTGGC CTCACCTATC CCAGAAGATG CTGGATCTGC TTGTGCCCAT GCCCAAAGCC 
TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAGCAG 
AGTAAGGTGA AGAAGCAGAG GCAGCAGCTG GAGGAACAGA AAAATGCCCC CATGTTCCAG 
CGCATGGAGC CTTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCTTAC 
CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGGAGTG GATACCCTTC GATGAGTCCA 
CTCTCTCCCC AGGATATATT CCAGTTGGCT TGTATGGACC CCGCCGATGA CGGACAGTTC 
CAAGAACGGC AGTCTCTGGT GGTGACAGAC CCTAGCTCCA TGAGACGTTC ATTTTCCACT 
ATTCGGGATA AGCGTTCAAA TTCCTCGTGG TTGGAGGAAT TCTCCATGGA GCGAAGCAGT 
GAAAATACCT ACAAGTCCCG TCGCCGGAGT TACCACTCCT CCTTGCGGCT GTCAGCCCAC 
CGCCTGAACT CTGATTCAGG CCACAAGTCT GACACTCACC GCTCAGGGGG CAGGGAGCGG 
GGACGATCAA AAGAGCGAAA GCATCTTCTC TCTCCTGATG TCTCCCGCTG CAATTCAGAA 
GAGCGAGGGA CCCAGGCTGA CTGGGAGTCC CCAGAGCGCC GTCAATCCAG GTCACCCAGT 
GAGGGCAGGT CACAGACGCC CAACAGACAG GGCACAGGTT CCCTAAGTGA GAGCTCCATC 
CCCTCTGTCT CTGACACCAG CACCCCAAGA AGAAGTCGTC GGCAGCTCCC ACCCGTCCCG 
CCAAAGCCCC GGCCCCTCCT TTCCTACAGC TCCCTGATTC GACACGCGGG CAGCATCTCT 
CCACCTGCTG ATGGAAGCGA GGAGGGCTCC CCGCTGACCT CCCAAGCTCT GGAGAGCAAC 
AATGCTTGCC TGACCGAGTC TTCCAACTCT CCGCACCCCC AGCAGAGCCA ACATGCCTCC 
CCACAGCGCT ACATCTCCGA GCCCTACTTG GCCCTGCACG AAGACTCCCA CGCCTCAGAC 
TGTGGTGAGG AGGAGACGCT CACTTTCGAA GCAGCCGTGG CTACTAGCCT GGGCCGTTCC 
AACACCATCG GCTCAGCCCC ACCCCTGCGG CATAGCTGGC AGATGCCCAA CGGGCACTAT 
CGGCGGCGGA GGCGCGGGGG GCCTGGGCCA GGCATGATGT GTGGGGCTGT CAACAACCTG 
CTAAGTGACA CGGAAGAAGA TGACAAATGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000712.2
CDS	196..7008
Translation	MARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFEDRARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTSVTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNNLLSDTEEDDKC

Target ORF information:

RefSeq Version	NM_000721.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000721.3

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG 
CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT 
GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG 
TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC 
AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC 
GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT 
CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG 
GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG 
ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG 
AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA 
AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA 
GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA 
CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC 
AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG 
ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC 
TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC 
AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC 
AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG 
AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA 
GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT 
GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC 
ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT 
ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA 
GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCGATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCCGT GTGGGAGCAG 
CGTACCAGCC AGCTGAGGAA GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAGAGAG 
GAGGCGCCGA CCATGAACCC GCTCAACCCC CTCAACCCGC TCAGCTCCCT CAACCCGCTC 
AATGCCCACC CCAGCCTTTA TCGGCGACCC AGGGCCATTG AGGGCCTGGC CCTGGGCCTG 
GCCCTGGAGA AGTTCGAGGA GGAGCGCATC AGCCGTGGGG GGTCCCTCAA GGGGGATGGA 
GGGGACCGAT CCAGTGCCCT GGACAACCAG AGGACCCCTT TGTCCCTGGG CCAGCGGGAG 
CCACCATGGC TGGCCAGGCC CTGTCATGGA AACTGTGACC CGACTCAGCA GGAGGCAGGG 
GGAGGAGAGG CTGTGGTGAC CTTTGAGGAC CGGGCCAGGC ACAGGCAGAG CCAACGGCGC 
AGCCGGCATC GCCGCGTCAG GACAGAAGGC AAGGAGTCCT CTTCAGCCTC CCGGAGCAGG 
TCTGCCAGCC AGGAACGCAG TCTGGATGAA GCCATGCCCA CTGAAGGGGA GAAGGACCAT 
GAGCTCAGGG GCAACCATGG TGCCAAGGAG CCAACGATCC AAGAAGAGAG AGCCCAGGAT 
TTAAGGAGGA CCAACAGTCT GATGGTGTCC AGAGGCTCCG GGCTGGCAGG AGGCCTTGAT 
GAGGCTGACA CCCCCCTAGT CCTGCCCCAT CCTGAGCTGG AAGTGGGGAA GCACGTGGTG 
CTGACGGAGC AGGAGCCAGA AGGCAGCAGT GAGCAGGCCC TGCTGGGGAA TGTGCAGCTA 
GACATGGGCC GGGTCATCAG CCAGAGCGAG CCTGACCTCT CCTGCATCAC GGCCAACACG 
GACAAGGCCA CCACCGAGAG CACCAGCGTC ACCGTCGCCA TCCCCGACGT GGACCCCTTG 
GTGGACTCAA CCGTGGTGCA CATTAGCAAC AAGACGGATG GGGAAGCCAG TCCCTTGAAG 
GAGGCAGAGA TCAGAGAGGA TGAGGAGGAG GTGGAGAAGA AGAAGCAGAA GAAGGAGAAG 
CGTGAGACAG GCAAAGCCAT GGTGCCCCAC AGCTCAATGT TCATCTTCAG CACCACCAAC 
CCGATCCGGA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATCCTC 
CTGGTGATTG CAGCCAGCAG CATCGCCCTG GCGGCAGAGG ACCCCGTCCT GACCAACTCG 
GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTGTTCA CGGGCGTGTT CACCTTTGAG 
ATGGTTATAA AGATGATAGA CCAAGGCTTG ATCCTGCAGG ATGGGTCCTA CTTCCGAGAC 
TTGTGGAACA TCCTGGACTT TGTGGTGGTC GTTGGCGCAT TGGTGGCCTT TGCTCTGGCG 
AACGCTTTGG GAACCAACAA AGGACGGGAC ATCAAGACCA TCAAGTCTCT GCGGGTGCTC 
CGAGTTCTAA GGCCACTGAA AACCATCAAG CGCTTGCCCA AGCTCAAGGC CGTCTTCGAC 
TGCGTAGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TTGTGTACAA GCTCTTCATG 
TTCATCTTTG CTGTCATCGC AGTTCAGCTC TTCAAGGGAA AGTTCTTTTA TTGCACGGAC 
AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTATG TAGATCATGA GAAAAACAAG 
ATGGAGGTGA AGGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG 
GCCCTGCTGA CCCTCTTCAC CGTCTCCACA GGGGAAGGAT GGCCTCAAGT TCTGCAGCAC 
TCTGTAGATG TGACAGAGGA AGACCGAGGC CCAAGCCGCA GCAACCGCAT GGAGATGTCT 
ATCTTTTATG TAGTCTACTT TGTGGTCTTC CCCTTCTTCT TTGTCAATAT CTTTGTGGCT 
CTCATCATCA TCACCTTCCA GGAGCAAGGG GATAAGATGA TGGAGGAGTG CAGCCTGGAG 
AAGAATGAGA GGGCGTGCAT CGACTTCGCC ATCAGCGCCA AACCTCTCAC CCGCTACATG 
CCGCAGAACA GACACACCTT CCAGTACCGC GTGTGGCACT TTGTGGTGTC TCCGTCCTTT 
GAGTACACCA TTATGGCCAT GATCGCCTTG AATACTGTTG TGCTGATGAT GAAGTATTAT 
TCTGCTCCCT GTACCTATGA GCTGGCCCTG AAGTACCTGA ATATCGCCTT CACCATGGTG 
TTTTCCCTGG AATGTGTCCT GAAGGTCATC GCTTTTGGCT TTTTGAACTA TTTCCGAGAC 
ACCTGGAATA TCTTTGACTT CATCACCGTG ATTGGCAGTA TCACAGAAAT TATCCTGACA 
GACAGCAAGC TGGTGAACAC CAGTGGCTTC AATATGAGCT TTCTGAAGCT CTTCCGAGCT 
GCCCGCCTCA TAAAGCTCCT GCGTCAGGGC TATACCATAC GCATTTTGCT GTGGACCTTT 
GTGCAGTCCT TTAAGGCCCT CCCTTATGTC TGCCTTTTAA TTGCCATGCT TTTCTTCATT 
TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGACGAGGA GAGTCACATC 
AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTAA TGCTACTCTT CAGGAGTGCC 
ACAGGTGAGG CCTGGCAGGA GATTATGCTG TCATGCCTTG GGGAGAAGGG CTGTGAGCCT 
GACACCACCG CACCATCAGG GCAGAACGAG AACGAACGCT GCGGCACCGA TCTGGCCTAC 
GTGTACTTTG TCTCCTTCAT CTTCTTCTGC TCCTTCTTGA TGCTCAACCT GTTTGTGGCC 
GTCATCATGG ACAACTTTGA GTACCTGACT CGGGACTCCT CCATCCTGGG GCCTCACCAC 
TTGGACGAGT TTGTCCGCGT CTGGGCAGAA TATGACCGAG CAGCATGTGG CCGCATCCAT 
TACACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCTC CGCTAGGCCT CGGCAAGAGA 
TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTGATGA ACATGCCAGT AGCTGAGGAC 
ATGACGGTCC ACTTCACCTC CACACTTATG GCTCTGATCC GGACAGCTCT GGACATTAAA 
ATTGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCAG AGCTACAAAA GGAGACCCTA 
GCCATCTGGC CTCACCTATC CCAGAAGATG CTGGATCTGC TTGTGCCCAT GCCCAAAGCC 
TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAGCAG 
AGTAAGGTGA AGAAGCAGAG GCAGCAGCTG GAGGAACAGA AAAATGCCCC CATGTTCCAG 
CGCATGGAGC CTTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCTTAC 
CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGGAGTG GATACCCTTC GATGAGTCCA 
CTCTCTCCCC AGGATATATT CCAGTTGGCT TGTATGGACC CCGCCGATGA CGGACAGTTC 
CAAGAACGGC AGTCTCTGGT GGTGACAGAC CCTAGCTCCA TGAGACGTTC ATTTTCCACT 
ATTCGGGATA AGCGTTCAAA TTCCTCGTGG TTGGAGGAAT TCTCCATGGA GCGAAGCAGT 
GAAAATACCT ACAAGTCCCG TCGCCGGAGT TACCACTCCT CCTTGCGGCT GTCAGCCCAC 
CGCCTGAACT CTGATTCAGG CCACAAGTCT GACACTCACC GCTCAGGGGG CAGGGAGCGG 
GGACGATCAA AAGAGCGAAA GCATCTTCTC TCTCCTGATG TCTCCCGCTG CAATTCAGAA 
GAGCGAGGGA CCCAGGCTGA CTGGGAGTCC CCAGAGCGCC GTCAATCCAG GTCACCCAGT 
GAGGGCAGGT CACAGACGCC CAACAGACAG GGCACAGGTT CCCTAAGTGA GAGCTCCATC 
CCCTCTGTCT CTGACACCAG CACCCCAAGA AGAAGTCGTC GGCAGCTCCC ACCCGTCCCG 
CCAAAGCCCC GGCCCCTCCT TTCCTACAGC TCCCTGATTC GACACGCGGG CAGCATCTCT 
CCACCTGCTG ATGGAAGCGA GGAGGGCTCC CCGCTGACCT CCCAAGCTCT GGAGAGCAAC 
AATGCTTGCC TGACCGAGTC TTCCAACTCT CCGCACCCCC AGCAGAGCCA ACATGCCTCC 
CCACAGCGCT ACATCTCCGA GCCCTACTTG GCCCTGCACG AAGACTCCCA CGCCTCAGAC 
TGTGGTGAGG AGGAGACGCT CACTTTCGAA GCAGCCGTGG CTACTAGCCT GGGCCGTTCC 
AACACCATCG GCTCAGCCCC ACCCCTGCGG CATAGCTGGC AGATGCCCAA CGGGCACTAT 
CGGCGGCGGA GGCGCGGGGG GCCTGGGCCA GGCATGATGT GTGGGGCTGT CAACAACCTG 
CTAAGTGACA CGGAAGAAGA TGACAAATGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( NM_001205294.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001192223.1 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 2, mRNA.

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CloneID	OHu10868
Clone ID Related Accession (Same CDS sequence)	NM_001205294.2 , NM_001205294.1
Accession Version	NM_001205294.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160059.7, BP230397.1, L29384.1, AK309070.1, AB209499.1, AL161734.12, BC037800.1 and KF510705.1. On Jun 1, 2019 this sequence version replaced NM_001205294.1. Transcript Variant: This variant (2) is missing two non-consecutive in-frame coding exons compared to variant 1. This results in a shorter isoform (2, also known as alpha-1E-1) missing two internal protein segments compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L29384.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG 
CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT 
GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG 
TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC 
AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC 
GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT 
CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG 
GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG 
ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG 
AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA 
AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA 
GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA 
CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC 
AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG 
ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC 
TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC 
AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC 
AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG 
AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA 
GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT 
GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC 
ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT 
ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA 
GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCGATCGA GAGGGAGCGG AGGCGCCGGC ACCACATGTC CGTGTGGGAG 
CAGCGTACCA GCCAGCTGAG GAAGCACATG CAGATGTCCA GCCAGGAGGC CCTCAACAGA 
GAGGAGGCGC CGACCATGAA CCCGCTCAAC CCCCTCAACC CGCTCAGCTC CCTCAACCCG 
CTCAATGCCC ACCCCAGCCT TTATCGGCGA CCCAGGGCCA TTGAGGGCCT GGCCCTGGGC 
CTGGCCCTGG AGAAGTTCGA GGAGGAGCGC ATCAGCCGTG GGGGGTCCCT CAAGGGGGAT 
GGAGGGGACC GATCCAGTGC CCTGGACAAC CAGAGGACCC CTTTGTCCCT GGGCCAGCGG 
GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGAAACTGTG ACCCGACTCA GCAGGAGGCA 
GGGGGAGGAG AGGCTGTGGT GACCTTTGAG GACCGGGCCA GGCACAGGCA GAGCCAACGG 
CGCAGCCGGC ATCGCCGCGT CAGGACAGAA GGCAAGGAGT CCTCTTCAGC CTCCCGGAGC 
AGGTCTGCCA GCCAGGAACG CAGTCTGGAT GAAGCCATGC CCACTGAAGG GGAGAAGGAC 
CATGAGCTCA GGGGCAACCA TGGTGCCAAG GAGCCAACGA TCCAAGAAGA GAGAGCCCAG 
GATTTAAGGA GGACCAACAG TCTGATGGTG TCCAGAGGCT CCGGGCTGGC AGGAGGCCTT 
GATGAGGCTG ACACCCCCCT AGTCCTGCCC CATCCTGAGC TGGAAGTGGG GAAGCACGTG 
GTGCTGACGG AGCAGGAGCC AGAAGGCAGC AGTGAGCAGG CCCTGCTGGG GAATGTGCAG 
CTAGACATGG GCCGGGTCAT CAGCCAGAGC GAGCCTGACC TCTCCTGCAT CACGGCCAAC 
ACGGACAAGG CCACCACCGA GAGCACCAGC GTCACCGTCG CCATCCCCGA CGTGGACCCC 
TTGGTGGACT CAACCGTGGT GCACATTAGC AACAAGACGG ATGGGGAAGC CAGTCCCTTG 
AAGGAGGCAG AGATCAGAGA GGATGAGGAG GAGGTGGAGA AGAAGAAGCA GAAGAAGGAG 
AAGCGTGAGA CAGGCAAAGC CATGGTGCCC CACAGCTCAA TGTTCATCTT CAGCACCACC 
AACCCGATCC GGAGGGCCTG CCACTACATC GTGAACCTGC GCTACTTTGA GATGTGCATC 
CTCCTGGTGA TTGCAGCCAG CAGCATCGCC CTGGCGGCAG AGGACCCCGT CCTGACCAAC 
TCGGAGCGCA ACAAAGTCCT GAGGTATTTT GACTATGTGT TCACGGGCGT GTTCACCTTT 
GAGATGGTTA TAAAGATGAT AGACCAAGGC TTGATCCTGC AGGATGGGTC CTACTTCCGA 
GACTTGTGGA ACATCCTGGA CTTTGTGGTG GTCGTTGGCG CATTGGTGGC CTTTGCTCTG 
GCGAACGCTT TGGGAACCAA CAAAGGACGG GACATCAAGA CCATCAAGTC TCTGCGGGTG 
CTCCGAGTTC TAAGGCCACT GAAAACCATC AAGCGCTTGC CCAAGCTCAA GGCCGTCTTC 
GACTGCGTAG TGACCTCCTT GAAGAATGTC TTCAACATAC TCATTGTGTA CAAGCTCTTC 
ATGTTCATCT TTGCTGTCAT CGCAGTTCAG CTCTTCAAGG GAAAGTTCTT TTATTGCACG 
GACAGTTCCA AGGACACAGA GAAGGAGTGC ATAGGCAACT ATGTAGATCA TGAGAAAAAC 
AAGATGGAGG TGAAGGGCCG GGAATGGAAG CGCCATGAAT TCCACTACGA CAACATTATC 
TGGGCCCTGC TGACCCTCTT CACCGTCTCC ACAGGGGAAG GATGGCCTCA AGTTCTGCAG 
CACTCTGTAG ATGTGACAGA GGAAGACCGA GGCCCAAGCC GCAGCAACCG CATGGAGATG 
TCTATCTTTT ATGTAGTCTA CTTTGTGGTC TTCCCCTTCT TCTTTGTCAA TATCTTTGTG 
GCTCTCATCA TCATCACCTT CCAGGAGCAA GGGGATAAGA TGATGGAGGA GTGCAGCCTG 
GAGAAGAATG AGAGGGCGTG CATCGACTTC GCCATCAGCG CCAAACCTCT CACCCGCTAC 
ATGCCGCAGA ACAGACACAC CTTCCAGTAC CGCGTGTGGC ACTTTGTGGT GTCTCCGTCC 
TTTGAGTACA CCATTATGGC CATGATCGCC TTGAATACTG TTGTGCTGAT GATGAAGTAT 
TATTCTGCTC CCTGTACCTA TGAGCTGGCC CTGAAGTACC TGAATATCGC CTTCACCATG 
GTGTTTTCCC TGGAATGTGT CCTGAAGGTC ATCGCTTTTG GCTTTTTGAA CTATTTCCGA 
GACACCTGGA ATATCTTTGA CTTCATCACC GTGATTGGCA GTATCACAGA AATTATCCTG 
ACAGACAGCA AGCTGGTGAA CACCAGTGGC TTCAATATGA GCTTTCTGAA GCTCTTCCGA 
GCTGCCCGCC TCATAAAGCT CCTGCGTCAG GGCTATACCA TACGCATTTT GCTGTGGACC 
TTTGTGCAGT CCTTTAAGGC CCTCCCTTAT GTCTGCCTTT TAATTGCCAT GCTTTTCTTC 
ATTTATGCCA TCATTGGGAT GCAGGTATTT GGAAACATAA AATTAGACGA GGAGAGTCAC 
ATCAACCGGC ACAACAACTT CCGGAGTTTC TTTGGGTCCC TAATGCTACT CTTCAGGAGT 
GCCACAGGTG AGGCCTGGCA GGAGATTATG CTGTCATGCC TTGGGGAGAA GGGCTGTGAG 
CCTGACACCA CCGCACCATC AGGGCAGAAC GAGAACGAAC GCTGCGGCAC CGATCTGGCC 
TACGTGTACT TTGTCTCCTT CATCTTCTTC TGCTCCTTCT TGATGCTCAA CCTGTTTGTG 
GCCGTCATCA TGGACAACTT TGAGTACCTG ACTCGGGACT CCTCCATCCT GGGGCCTCAC 
CACTTGGACG AGTTTGTCCG CGTCTGGGCA GAATATGACC GAGCAGCATG TGGCCGCATC 
CATTACACTG AGATGTATGA AATGCTGACT CTCATGTCAC CTCCGCTAGG CCTCGGCAAG 
AGATGTCCCT CCAAAGTGGC ATATAAGAGG TTGGTCCTGA TGAACATGCC AGTAGCTGAG 
GACATGACGG TCCACTTCAC CTCCACACTT ATGGCTCTGA TCCGGACAGC TCTGGACATT 
AAAATTGCCA AAGGTGGTGC AGACAGGCAG CAGCTAGACT CAGAGCTACA AAAGGAGACC 
CTAGCCATCT GGCCTCACCT ATCCCAGAAG ATGCTGGATC TGCTTGTGCC CATGCCCAAA 
GCCTCTGACC TGACTGTGGG CAAAATCTAT GCAGCAATGA TGATCATGGA CTACTATAAG 
CAGAGTAAGG TGAAGAAGCA GAGGCAGCAG CTGGAGGAAC AGAAAAATGC CCCCATGTTC 
CAGCGCATGG AGCCTTCATC TCTGCCTCAG GAGATCATTG CTAATGCCAA AGCCCTGCCT 
TACCTCCAGC AGGACCCCGT TTCAGGCCTG AGTGGCCGGA GTGGATACCC TTCGATGAGT 
CCACTCTCTC CCCAGGATAT ATTCCAGTTG GCTTGTATGG ACCCCGCCGA TGACGGACAG 
TTCCAAGAAC GGCAGTCTCT GGTGGTGACA GACCCTAGCT CCATGAGACG TTCATTTTCC 
ACTATTCGGG ATAAGCGTTC AAATTCCTCG TGGTTGGAGG AATTCTCCAT GGAGCGAAGC 
AGTGAAAATA CCTACAAGTC CCGTCGCCGG AGTTACCACT CCTCCTTGCG GCTGTCAGCC 
CACCGCCTGA ACTCTGATTC AGGCCACAAG TCTGACACTC ACCGCTCAGG GGGCAGGGAG 
CGGGGACGAT CAAAAGAGCG AAAGCATCTT CTCTCTCCTG ATGTCTCCCG CTGCAATTCA 
GAAGAGCGAG GGACCCAGGC TGACTGGGAG TCCCCAGAGC GCCGTCAATC CAGGTCACCC 
AGTGAGGGCA GGTCACAGAC GCCCAACAGA CAGGGCACAG GTTCCCTAAG TGAGAGCTCC 
ATCCCCTCTG TCTCTGACAC CAGCACCCCA AGAAGAAGTC GTCGGCAGCT CCCACCCGTC 
CCGCCAAAGC CCCGGCCCCT CCTTTCCTAC AGCTCCCTGA TTCGACACGC GGGCAGCATC 
TCTCCACCTG CTGATGGAAG CGAGGAGGGC TCCCCGCTGA CCTCCCAAGC TCTGGAGAGC 
AACAATGCTT GCCTGACCGA GTCTTCCAAC TCTCCGCACC CCCAGCAGAG CCAACATGCC 
TCCCCACAGC GCTACATCTC CGAGCCCTAC TTGGCCCTGC ACGAAGACTC CCACGCCTCA 
GACTGTGGTG AGGAGGAGAC GCTCACTTTC GAAGCAGCCG TGGCTACTAG CCTGGGCCGT 
TCCAACACCA TCGGCTCAGC CCCACCCCTG CGGCATAGCT GGCAGATGCC CAACGGGCAC 
TATCGGCGGC GGAGGCGCGG GGGGCCTGGG CCAGGCATGA TGTGTGGGGC TGTCAACAAC 
CTGCTAAGTG ACACGGAAGA AGATGACAAA TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192223.1
CDS	229..6984
Translation	MARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFEDRARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTSVTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNNLLSDTEEDDKC

Target ORF information:

RefSeq Version	NM_001205294.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205294.2

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG 
CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT 
GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG 
TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC 
AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC 
GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT 
CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG 
GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG 
ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG 
AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA 
AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA 
GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA 
CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC 
AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG 
ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC 
TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC 
AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC 
AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG 
AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA 
GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT 
GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC 
ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT 
ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA 
GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCGATCGA GAGGGAGCGG AGGCGCCGGC ACCACATGTC CGTGTGGGAG 
CAGCGTACCA GCCAGCTGAG GAAGCACATG CAGATGTCCA GCCAGGAGGC CCTCAACAGA 
GAGGAGGCGC CGACCATGAA CCCGCTCAAC CCCCTCAACC CGCTCAGCTC CCTCAACCCG 
CTCAATGCCC ACCCCAGCCT TTATCGGCGA CCCAGGGCCA TTGAGGGCCT GGCCCTGGGC 
CTGGCCCTGG AGAAGTTCGA GGAGGAGCGC ATCAGCCGTG GGGGGTCCCT CAAGGGGGAT 
GGAGGGGACC GATCCAGTGC CCTGGACAAC CAGAGGACCC CTTTGTCCCT GGGCCAGCGG 
GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGAAACTGTG ACCCGACTCA GCAGGAGGCA 
GGGGGAGGAG AGGCTGTGGT GACCTTTGAG GACCGGGCCA GGCACAGGCA GAGCCAACGG 
CGCAGCCGGC ATCGCCGCGT CAGGACAGAA GGCAAGGAGT CCTCTTCAGC CTCCCGGAGC 
AGGTCTGCCA GCCAGGAACG CAGTCTGGAT GAAGCCATGC CCACTGAAGG GGAGAAGGAC 
CATGAGCTCA GGGGCAACCA TGGTGCCAAG GAGCCAACGA TCCAAGAAGA GAGAGCCCAG 
GATTTAAGGA GGACCAACAG TCTGATGGTG TCCAGAGGCT CCGGGCTGGC AGGAGGCCTT 
GATGAGGCTG ACACCCCCCT AGTCCTGCCC CATCCTGAGC TGGAAGTGGG GAAGCACGTG 
GTGCTGACGG AGCAGGAGCC AGAAGGCAGC AGTGAGCAGG CCCTGCTGGG GAATGTGCAG 
CTAGACATGG GCCGGGTCAT CAGCCAGAGC GAGCCTGACC TCTCCTGCAT CACGGCCAAC 
ACGGACAAGG CCACCACCGA GAGCACCAGC GTCACCGTCG CCATCCCCGA CGTGGACCCC 
TTGGTGGACT CAACCGTGGT GCACATTAGC AACAAGACGG ATGGGGAAGC CAGTCCCTTG 
AAGGAGGCAG AGATCAGAGA GGATGAGGAG GAGGTGGAGA AGAAGAAGCA GAAGAAGGAG 
AAGCGTGAGA CAGGCAAAGC CATGGTGCCC CACAGCTCAA TGTTCATCTT CAGCACCACC 
AACCCGATCC GGAGGGCCTG CCACTACATC GTGAACCTGC GCTACTTTGA GATGTGCATC 
CTCCTGGTGA TTGCAGCCAG CAGCATCGCC CTGGCGGCAG AGGACCCCGT CCTGACCAAC 
TCGGAGCGCA ACAAAGTCCT GAGGTATTTT GACTATGTGT TCACGGGCGT GTTCACCTTT 
GAGATGGTTA TAAAGATGAT AGACCAAGGC TTGATCCTGC AGGATGGGTC CTACTTCCGA 
GACTTGTGGA ACATCCTGGA CTTTGTGGTG GTCGTTGGCG CATTGGTGGC CTTTGCTCTG 
GCGAACGCTT TGGGAACCAA CAAAGGACGG GACATCAAGA CCATCAAGTC TCTGCGGGTG 
CTCCGAGTTC TAAGGCCACT GAAAACCATC AAGCGCTTGC CCAAGCTCAA GGCCGTCTTC 
GACTGCGTAG TGACCTCCTT GAAGAATGTC TTCAACATAC TCATTGTGTA CAAGCTCTTC 
ATGTTCATCT TTGCTGTCAT CGCAGTTCAG CTCTTCAAGG GAAAGTTCTT TTATTGCACG 
GACAGTTCCA AGGACACAGA GAAGGAGTGC ATAGGCAACT ATGTAGATCA TGAGAAAAAC 
AAGATGGAGG TGAAGGGCCG GGAATGGAAG CGCCATGAAT TCCACTACGA CAACATTATC 
TGGGCCCTGC TGACCCTCTT CACCGTCTCC ACAGGGGAAG GATGGCCTCA AGTTCTGCAG 
CACTCTGTAG ATGTGACAGA GGAAGACCGA GGCCCAAGCC GCAGCAACCG CATGGAGATG 
TCTATCTTTT ATGTAGTCTA CTTTGTGGTC TTCCCCTTCT TCTTTGTCAA TATCTTTGTG 
GCTCTCATCA TCATCACCTT CCAGGAGCAA GGGGATAAGA TGATGGAGGA GTGCAGCCTG 
GAGAAGAATG AGAGGGCGTG CATCGACTTC GCCATCAGCG CCAAACCTCT CACCCGCTAC 
ATGCCGCAGA ACAGACACAC CTTCCAGTAC CGCGTGTGGC ACTTTGTGGT GTCTCCGTCC 
TTTGAGTACA CCATTATGGC CATGATCGCC TTGAATACTG TTGTGCTGAT GATGAAGTAT 
TATTCTGCTC CCTGTACCTA TGAGCTGGCC CTGAAGTACC TGAATATCGC CTTCACCATG 
GTGTTTTCCC TGGAATGTGT CCTGAAGGTC ATCGCTTTTG GCTTTTTGAA CTATTTCCGA 
GACACCTGGA ATATCTTTGA CTTCATCACC GTGATTGGCA GTATCACAGA AATTATCCTG 
ACAGACAGCA AGCTGGTGAA CACCAGTGGC TTCAATATGA GCTTTCTGAA GCTCTTCCGA 
GCTGCCCGCC TCATAAAGCT CCTGCGTCAG GGCTATACCA TACGCATTTT GCTGTGGACC 
TTTGTGCAGT CCTTTAAGGC CCTCCCTTAT GTCTGCCTTT TAATTGCCAT GCTTTTCTTC 
ATTTATGCCA TCATTGGGAT GCAGGTATTT GGAAACATAA AATTAGACGA GGAGAGTCAC 
ATCAACCGGC ACAACAACTT CCGGAGTTTC TTTGGGTCCC TAATGCTACT CTTCAGGAGT 
GCCACAGGTG AGGCCTGGCA GGAGATTATG CTGTCATGCC TTGGGGAGAA GGGCTGTGAG 
CCTGACACCA CCGCACCATC AGGGCAGAAC GAGAACGAAC GCTGCGGCAC CGATCTGGCC 
TACGTGTACT TTGTCTCCTT CATCTTCTTC TGCTCCTTCT TGATGCTCAA CCTGTTTGTG 
GCCGTCATCA TGGACAACTT TGAGTACCTG ACTCGGGACT CCTCCATCCT GGGGCCTCAC 
CACTTGGACG AGTTTGTCCG CGTCTGGGCA GAATATGACC GAGCAGCATG TGGCCGCATC 
CATTACACTG AGATGTATGA AATGCTGACT CTCATGTCAC CTCCGCTAGG CCTCGGCAAG 
AGATGTCCCT CCAAAGTGGC ATATAAGAGG TTGGTCCTGA TGAACATGCC AGTAGCTGAG 
GACATGACGG TCCACTTCAC CTCCACACTT ATGGCTCTGA TCCGGACAGC TCTGGACATT 
AAAATTGCCA AAGGTGGTGC AGACAGGCAG CAGCTAGACT CAGAGCTACA AAAGGAGACC 
CTAGCCATCT GGCCTCACCT ATCCCAGAAG ATGCTGGATC TGCTTGTGCC CATGCCCAAA 
GCCTCTGACC TGACTGTGGG CAAAATCTAT GCAGCAATGA TGATCATGGA CTACTATAAG 
CAGAGTAAGG TGAAGAAGCA GAGGCAGCAG CTGGAGGAAC AGAAAAATGC CCCCATGTTC 
CAGCGCATGG AGCCTTCATC TCTGCCTCAG GAGATCATTG CTAATGCCAA AGCCCTGCCT 
TACCTCCAGC AGGACCCCGT TTCAGGCCTG AGTGGCCGGA GTGGATACCC TTCGATGAGT 
CCACTCTCTC CCCAGGATAT ATTCCAGTTG GCTTGTATGG ACCCCGCCGA TGACGGACAG 
TTCCAAGAAC GGCAGTCTCT GGTGGTGACA GACCCTAGCT CCATGAGACG TTCATTTTCC 
ACTATTCGGG ATAAGCGTTC AAATTCCTCG TGGTTGGAGG AATTCTCCAT GGAGCGAAGC 
AGTGAAAATA CCTACAAGTC CCGTCGCCGG AGTTACCACT CCTCCTTGCG GCTGTCAGCC 
CACCGCCTGA ACTCTGATTC AGGCCACAAG TCTGACACTC ACCGCTCAGG GGGCAGGGAG 
CGGGGACGAT CAAAAGAGCG AAAGCATCTT CTCTCTCCTG ATGTCTCCCG CTGCAATTCA 
GAAGAGCGAG GGACCCAGGC TGACTGGGAG TCCCCAGAGC GCCGTCAATC CAGGTCACCC 
AGTGAGGGCA GGTCACAGAC GCCCAACAGA CAGGGCACAG GTTCCCTAAG TGAGAGCTCC 
ATCCCCTCTG TCTCTGACAC CAGCACCCCA AGAAGAAGTC GTCGGCAGCT CCCACCCGTC 
CCGCCAAAGC CCCGGCCCCT CCTTTCCTAC AGCTCCCTGA TTCGACACGC GGGCAGCATC 
TCTCCACCTG CTGATGGAAG CGAGGAGGGC TCCCCGCTGA CCTCCCAAGC TCTGGAGAGC 
AACAATGCTT GCCTGACCGA GTCTTCCAAC TCTCCGCACC CCCAGCAGAG CCAACATGCC 
TCCCCACAGC GCTACATCTC CGAGCCCTAC TTGGCCCTGC ACGAAGACTC CCACGCCTCA 
GACTGTGGTG AGGAGGAGAC GCTCACTTTC GAAGCAGCCG TGGCTACTAG CCTGGGCCGT 
TCCAACACCA TCGGCTCAGC CCCACCCCTG CGGCATAGCT GGCAGATGCC CAACGGGCAC 
TATCGGCGGC GGAGGCGCGG GGGGCCTGGG CCAGGCATGA TGTGTGGGGC TGTCAACAAC 
CTGCTAAGTG ACACGGAAGA AGATGACAAA TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( NM_001205294.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001192223.1 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 2, mRNA.

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CloneID	OHu10868
Clone ID Related Accession (Same CDS sequence)	NM_001205294.2 , NM_001205294.1
Accession Version	NM_001205294.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-31
Organism	Homo sapiens(human)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP230397.1, L29384.1, AK309070.1, AB209499.1, AL161734.12 and BC037800.1. Transcript Variant: This variant (2) is missing two non-consecutive in-frame coding exons compared to variant 1. This results in a shorter isoform (2, also known as alpha-1E-1) missing two internal protein segments compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L29384.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG 
CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT 
GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG 
TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC 
AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC 
GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT 
CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG 
GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG 
ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG 
AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA 
AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA 
GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA 
CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC 
AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG 
ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC 
TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC 
AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC 
AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG 
AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA 
GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT 
GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC 
ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT 
ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA 
GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCGATCGA GAGGGAGCGG AGGCGCCGGC ACCACATGTC CGTGTGGGAG 
CAGCGTACCA GCCAGCTGAG GAAGCACATG CAGATGTCCA GCCAGGAGGC CCTCAACAGA 
GAGGAGGCGC CGACCATGAA CCCGCTCAAC CCCCTCAACC CGCTCAGCTC CCTCAACCCG 
CTCAATGCCC ACCCCAGCCT TTATCGGCGA CCCAGGGCCA TTGAGGGCCT GGCCCTGGGC 
CTGGCCCTGG AGAAGTTCGA GGAGGAGCGC ATCAGCCGTG GGGGGTCCCT CAAGGGGGAT 
GGAGGGGACC GATCCAGTGC CCTGGACAAC CAGAGGACCC CTTTGTCCCT GGGCCAGCGG 
GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGAAACTGTG ACCCGACTCA GCAGGAGGCA 
GGGGGAGGAG AGGCTGTGGT GACCTTTGAG GACCGGGCCA GGCACAGGCA GAGCCAACGG 
CGCAGCCGGC ATCGCCGCGT CAGGACAGAA GGCAAGGAGT CCTCTTCAGC CTCCCGGAGC 
AGGTCTGCCA GCCAGGAACG CAGTCTGGAT GAAGCCATGC CCACTGAAGG GGAGAAGGAC 
CATGAGCTCA GGGGCAACCA TGGTGCCAAG GAGCCAACGA TCCAAGAAGA GAGAGCCCAG 
GATTTAAGGA GGACCAACAG TCTGATGGTG TCCAGAGGCT CCGGGCTGGC AGGAGGCCTT 
GATGAGGCTG ACACCCCCCT AGTCCTGCCC CATCCTGAGC TGGAAGTGGG GAAGCACGTG 
GTGCTGACGG AGCAGGAGCC AGAAGGCAGC AGTGAGCAGG CCCTGCTGGG GAATGTGCAG 
CTAGACATGG GCCGGGTCAT CAGCCAGAGC GAGCCTGACC TCTCCTGCAT CACGGCCAAC 
ACGGACAAGG CCACCACCGA GAGCACCAGC GTCACCGTCG CCATCCCCGA CGTGGACCCC 
TTGGTGGACT CAACCGTGGT GCACATTAGC AACAAGACGG ATGGGGAAGC CAGTCCCTTG 
AAGGAGGCAG AGATCAGAGA GGATGAGGAG GAGGTGGAGA AGAAGAAGCA GAAGAAGGAG 
AAGCGTGAGA CAGGCAAAGC CATGGTGCCC CACAGCTCAA TGTTCATCTT CAGCACCACC 
AACCCGATCC GGAGGGCCTG CCACTACATC GTGAACCTGC GCTACTTTGA GATGTGCATC 
CTCCTGGTGA TTGCAGCCAG CAGCATCGCC CTGGCGGCAG AGGACCCCGT CCTGACCAAC 
TCGGAGCGCA ACAAAGTCCT GAGGTATTTT GACTATGTGT TCACGGGCGT GTTCACCTTT 
GAGATGGTTA TAAAGATGAT AGACCAAGGC TTGATCCTGC AGGATGGGTC CTACTTCCGA 
GACTTGTGGA ACATCCTGGA CTTTGTGGTG GTCGTTGGCG CATTGGTGGC CTTTGCTCTG 
GCGAACGCTT TGGGAACCAA CAAAGGACGG GACATCAAGA CCATCAAGTC TCTGCGGGTG 
CTCCGAGTTC TAAGGCCACT GAAAACCATC AAGCGCTTGC CCAAGCTCAA GGCCGTCTTC 
GACTGCGTAG TGACCTCCTT GAAGAATGTC TTCAACATAC TCATTGTGTA CAAGCTCTTC 
ATGTTCATCT TTGCTGTCAT CGCAGTTCAG CTCTTCAAGG GAAAGTTCTT TTATTGCACG 
GACAGTTCCA AGGACACAGA GAAGGAGTGC ATAGGCAACT ATGTAGATCA TGAGAAAAAC 
AAGATGGAGG TGAAGGGCCG GGAATGGAAG CGCCATGAAT TCCACTACGA CAACATTATC 
TGGGCCCTGC TGACCCTCTT CACCGTCTCC ACAGGGGAAG GATGGCCTCA AGTTCTGCAG 
CACTCTGTAG ATGTGACAGA GGAAGACCGA GGCCCAAGCC GCAGCAACCG CATGGAGATG 
TCTATCTTTT ATGTAGTCTA CTTTGTGGTC TTCCCCTTCT TCTTTGTCAA TATCTTTGTG 
GCTCTCATCA TCATCACCTT CCAGGAGCAA GGGGATAAGA TGATGGAGGA GTGCAGCCTG 
GAGAAGAATG AGAGGGCGTG CATCGACTTC GCCATCAGCG CCAAACCTCT CACCCGCTAC 
ATGCCGCAGA ACAGACACAC CTTCCAGTAC CGCGTGTGGC ACTTTGTGGT GTCTCCGTCC 
TTTGAGTACA CCATTATGGC CATGATCGCC TTGAATACTG TTGTGCTGAT GATGAAGTAT 
TATTCTGCTC CCTGTACCTA TGAGCTGGCC CTGAAGTACC TGAATATCGC CTTCACCATG 
GTGTTTTCCC TGGAATGTGT CCTGAAGGTC ATCGCTTTTG GCTTTTTGAA CTATTTCCGA 
GACACCTGGA ATATCTTTGA CTTCATCACC GTGATTGGCA GTATCACAGA AATTATCCTG 
ACAGACAGCA AGCTGGTGAA CACCAGTGGC TTCAATATGA GCTTTCTGAA GCTCTTCCGA 
GCTGCCCGCC TCATAAAGCT CCTGCGTCAG GGCTATACCA TACGCATTTT GCTGTGGACC 
TTTGTGCAGT CCTTTAAGGC CCTCCCTTAT GTCTGCCTTT TAATTGCCAT GCTTTTCTTC 
ATTTATGCCA TCATTGGGAT GCAGGTATTT GGAAACATAA AATTAGACGA GGAGAGTCAC 
ATCAACCGGC ACAACAACTT CCGGAGTTTC TTTGGGTCCC TAATGCTACT CTTCAGGAGT 
GCCACAGGTG AGGCCTGGCA GGAGATTATG CTGTCATGCC TTGGGGAGAA GGGCTGTGAG 
CCTGACACCA CCGCACCATC AGGGCAGAAC GAGAACGAAC GCTGCGGCAC CGATCTGGCC 
TACGTGTACT TTGTCTCCTT CATCTTCTTC TGCTCCTTCT TGATGCTCAA CCTGTTTGTG 
GCCGTCATCA TGGACAACTT TGAGTACCTG ACTCGGGACT CCTCCATCCT GGGGCCTCAC 
CACTTGGACG AGTTTGTCCG CGTCTGGGCA GAATATGACC GAGCAGCATG TGGCCGCATC 
CATTACACTG AGATGTATGA AATGCTGACT CTCATGTCAC CTCCGCTAGG CCTCGGCAAG 
AGATGTCCCT CCAAAGTGGC ATATAAGAGG TTGGTCCTGA TGAACATGCC AGTAGCTGAG 
GACATGACGG TCCACTTCAC CTCCACACTT ATGGCTCTGA TCCGGACAGC TCTGGACATT 
AAAATTGCCA AAGGTGGTGC AGACAGGCAG CAGCTAGACT CAGAGCTACA AAAGGAGACC 
CTAGCCATCT GGCCTCACCT ATCCCAGAAG ATGCTGGATC TGCTTGTGCC CATGCCCAAA 
GCCTCTGACC TGACTGTGGG CAAAATCTAT GCAGCAATGA TGATCATGGA CTACTATAAG 
CAGAGTAAGG TGAAGAAGCA GAGGCAGCAG CTGGAGGAAC AGAAAAATGC CCCCATGTTC 
CAGCGCATGG AGCCTTCATC TCTGCCTCAG GAGATCATTG CTAATGCCAA AGCCCTGCCT 
TACCTCCAGC AGGACCCCGT TTCAGGCCTG AGTGGCCGGA GTGGATACCC TTCGATGAGT 
CCACTCTCTC CCCAGGATAT ATTCCAGTTG GCTTGTATGG ACCCCGCCGA TGACGGACAG 
TTCCAAGAAC GGCAGTCTCT GGTGGTGACA GACCCTAGCT CCATGAGACG TTCATTTTCC 
ACTATTCGGG ATAAGCGTTC AAATTCCTCG TGGTTGGAGG AATTCTCCAT GGAGCGAAGC 
AGTGAAAATA CCTACAAGTC CCGTCGCCGG AGTTACCACT CCTCCTTGCG GCTGTCAGCC 
CACCGCCTGA ACTCTGATTC AGGCCACAAG TCTGACACTC ACCGCTCAGG GGGCAGGGAG 
CGGGGACGAT CAAAAGAGCG AAAGCATCTT CTCTCTCCTG ATGTCTCCCG CTGCAATTCA 
GAAGAGCGAG GGACCCAGGC TGACTGGGAG TCCCCAGAGC GCCGTCAATC CAGGTCACCC 
AGTGAGGGCA GGTCACAGAC GCCCAACAGA CAGGGCACAG GTTCCCTAAG TGAGAGCTCC 
ATCCCCTCTG TCTCTGACAC CAGCACCCCA AGAAGAAGTC GTCGGCAGCT CCCACCCGTC 
CCGCCAAAGC CCCGGCCCCT CCTTTCCTAC AGCTCCCTGA TTCGACACGC GGGCAGCATC 
TCTCCACCTG CTGATGGAAG CGAGGAGGGC TCCCCGCTGA CCTCCCAAGC TCTGGAGAGC 
AACAATGCTT GCCTGACCGA GTCTTCCAAC TCTCCGCACC CCCAGCAGAG CCAACATGCC 
TCCCCACAGC GCTACATCTC CGAGCCCTAC TTGGCCCTGC ACGAAGACTC CCACGCCTCA 
GACTGTGGTG AGGAGGAGAC GCTCACTTTC GAAGCAGCCG TGGCTACTAG CCTGGGCCGT 
TCCAACACCA TCGGCTCAGC CCCACCCCTG CGGCATAGCT GGCAGATGCC CAACGGGCAC 
TATCGGCGGC GGAGGCGCGG GGGGCCTGGG CCAGGCATGA TGTGTGGGGC TGTCAACAAC 
CTGCTAAGTG ACACGGAAGA AGATGACAAA TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192223.1
CDS	196..6951
Translation	MARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFEDRARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTSVTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNNLLSDTEEDDKC

Target ORF information:

RefSeq Version	NM_001205294.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205294.1

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG 
CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT 
GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG 
TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC 
AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC 
GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT 
CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG 
GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG 
ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG 
AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA 
AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA 
GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA 
CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC 
AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG 
ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC 
TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC 
AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC 
AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG 
AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA 
GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT 
GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC 
ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT 
ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA 
GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCGATCGA GAGGGAGCGG AGGCGCCGGC ACCACATGTC CGTGTGGGAG 
CAGCGTACCA GCCAGCTGAG GAAGCACATG CAGATGTCCA GCCAGGAGGC CCTCAACAGA 
GAGGAGGCGC CGACCATGAA CCCGCTCAAC CCCCTCAACC CGCTCAGCTC CCTCAACCCG 
CTCAATGCCC ACCCCAGCCT TTATCGGCGA CCCAGGGCCA TTGAGGGCCT GGCCCTGGGC 
CTGGCCCTGG AGAAGTTCGA GGAGGAGCGC ATCAGCCGTG GGGGGTCCCT CAAGGGGGAT 
GGAGGGGACC GATCCAGTGC CCTGGACAAC CAGAGGACCC CTTTGTCCCT GGGCCAGCGG 
GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGAAACTGTG ACCCGACTCA GCAGGAGGCA 
GGGGGAGGAG AGGCTGTGGT GACCTTTGAG GACCGGGCCA GGCACAGGCA GAGCCAACGG 
CGCAGCCGGC ATCGCCGCGT CAGGACAGAA GGCAAGGAGT CCTCTTCAGC CTCCCGGAGC 
AGGTCTGCCA GCCAGGAACG CAGTCTGGAT GAAGCCATGC CCACTGAAGG GGAGAAGGAC 
CATGAGCTCA GGGGCAACCA TGGTGCCAAG GAGCCAACGA TCCAAGAAGA GAGAGCCCAG 
GATTTAAGGA GGACCAACAG TCTGATGGTG TCCAGAGGCT CCGGGCTGGC AGGAGGCCTT 
GATGAGGCTG ACACCCCCCT AGTCCTGCCC CATCCTGAGC TGGAAGTGGG GAAGCACGTG 
GTGCTGACGG AGCAGGAGCC AGAAGGCAGC AGTGAGCAGG CCCTGCTGGG GAATGTGCAG 
CTAGACATGG GCCGGGTCAT CAGCCAGAGC GAGCCTGACC TCTCCTGCAT CACGGCCAAC 
ACGGACAAGG CCACCACCGA GAGCACCAGC GTCACCGTCG CCATCCCCGA CGTGGACCCC 
TTGGTGGACT CAACCGTGGT GCACATTAGC AACAAGACGG ATGGGGAAGC CAGTCCCTTG 
AAGGAGGCAG AGATCAGAGA GGATGAGGAG GAGGTGGAGA AGAAGAAGCA GAAGAAGGAG 
AAGCGTGAGA CAGGCAAAGC CATGGTGCCC CACAGCTCAA TGTTCATCTT CAGCACCACC 
AACCCGATCC GGAGGGCCTG CCACTACATC GTGAACCTGC GCTACTTTGA GATGTGCATC 
CTCCTGGTGA TTGCAGCCAG CAGCATCGCC CTGGCGGCAG AGGACCCCGT CCTGACCAAC 
TCGGAGCGCA ACAAAGTCCT GAGGTATTTT GACTATGTGT TCACGGGCGT GTTCACCTTT 
GAGATGGTTA TAAAGATGAT AGACCAAGGC TTGATCCTGC AGGATGGGTC CTACTTCCGA 
GACTTGTGGA ACATCCTGGA CTTTGTGGTG GTCGTTGGCG CATTGGTGGC CTTTGCTCTG 
GCGAACGCTT TGGGAACCAA CAAAGGACGG GACATCAAGA CCATCAAGTC TCTGCGGGTG 
CTCCGAGTTC TAAGGCCACT GAAAACCATC AAGCGCTTGC CCAAGCTCAA GGCCGTCTTC 
GACTGCGTAG TGACCTCCTT GAAGAATGTC TTCAACATAC TCATTGTGTA CAAGCTCTTC 
ATGTTCATCT TTGCTGTCAT CGCAGTTCAG CTCTTCAAGG GAAAGTTCTT TTATTGCACG 
GACAGTTCCA AGGACACAGA GAAGGAGTGC ATAGGCAACT ATGTAGATCA TGAGAAAAAC 
AAGATGGAGG TGAAGGGCCG GGAATGGAAG CGCCATGAAT TCCACTACGA CAACATTATC 
TGGGCCCTGC TGACCCTCTT CACCGTCTCC ACAGGGGAAG GATGGCCTCA AGTTCTGCAG 
CACTCTGTAG ATGTGACAGA GGAAGACCGA GGCCCAAGCC GCAGCAACCG CATGGAGATG 
TCTATCTTTT ATGTAGTCTA CTTTGTGGTC TTCCCCTTCT TCTTTGTCAA TATCTTTGTG 
GCTCTCATCA TCATCACCTT CCAGGAGCAA GGGGATAAGA TGATGGAGGA GTGCAGCCTG 
GAGAAGAATG AGAGGGCGTG CATCGACTTC GCCATCAGCG CCAAACCTCT CACCCGCTAC 
ATGCCGCAGA ACAGACACAC CTTCCAGTAC CGCGTGTGGC ACTTTGTGGT GTCTCCGTCC 
TTTGAGTACA CCATTATGGC CATGATCGCC TTGAATACTG TTGTGCTGAT GATGAAGTAT 
TATTCTGCTC CCTGTACCTA TGAGCTGGCC CTGAAGTACC TGAATATCGC CTTCACCATG 
GTGTTTTCCC TGGAATGTGT CCTGAAGGTC ATCGCTTTTG GCTTTTTGAA CTATTTCCGA 
GACACCTGGA ATATCTTTGA CTTCATCACC GTGATTGGCA GTATCACAGA AATTATCCTG 
ACAGACAGCA AGCTGGTGAA CACCAGTGGC TTCAATATGA GCTTTCTGAA GCTCTTCCGA 
GCTGCCCGCC TCATAAAGCT CCTGCGTCAG GGCTATACCA TACGCATTTT GCTGTGGACC 
TTTGTGCAGT CCTTTAAGGC CCTCCCTTAT GTCTGCCTTT TAATTGCCAT GCTTTTCTTC 
ATTTATGCCA TCATTGGGAT GCAGGTATTT GGAAACATAA AATTAGACGA GGAGAGTCAC 
ATCAACCGGC ACAACAACTT CCGGAGTTTC TTTGGGTCCC TAATGCTACT CTTCAGGAGT 
GCCACAGGTG AGGCCTGGCA GGAGATTATG CTGTCATGCC TTGGGGAGAA GGGCTGTGAG 
CCTGACACCA CCGCACCATC AGGGCAGAAC GAGAACGAAC GCTGCGGCAC CGATCTGGCC 
TACGTGTACT TTGTCTCCTT CATCTTCTTC TGCTCCTTCT TGATGCTCAA CCTGTTTGTG 
GCCGTCATCA TGGACAACTT TGAGTACCTG ACTCGGGACT CCTCCATCCT GGGGCCTCAC 
CACTTGGACG AGTTTGTCCG CGTCTGGGCA GAATATGACC GAGCAGCATG TGGCCGCATC 
CATTACACTG AGATGTATGA AATGCTGACT CTCATGTCAC CTCCGCTAGG CCTCGGCAAG 
AGATGTCCCT CCAAAGTGGC ATATAAGAGG TTGGTCCTGA TGAACATGCC AGTAGCTGAG 
GACATGACGG TCCACTTCAC CTCCACACTT ATGGCTCTGA TCCGGACAGC TCTGGACATT 
AAAATTGCCA AAGGTGGTGC AGACAGGCAG CAGCTAGACT CAGAGCTACA AAAGGAGACC 
CTAGCCATCT GGCCTCACCT ATCCCAGAAG ATGCTGGATC TGCTTGTGCC CATGCCCAAA 
GCCTCTGACC TGACTGTGGG CAAAATCTAT GCAGCAATGA TGATCATGGA CTACTATAAG 
CAGAGTAAGG TGAAGAAGCA GAGGCAGCAG CTGGAGGAAC AGAAAAATGC CCCCATGTTC 
CAGCGCATGG AGCCTTCATC TCTGCCTCAG GAGATCATTG CTAATGCCAA AGCCCTGCCT 
TACCTCCAGC AGGACCCCGT TTCAGGCCTG AGTGGCCGGA GTGGATACCC TTCGATGAGT 
CCACTCTCTC CCCAGGATAT ATTCCAGTTG GCTTGTATGG ACCCCGCCGA TGACGGACAG 
TTCCAAGAAC GGCAGTCTCT GGTGGTGACA GACCCTAGCT CCATGAGACG TTCATTTTCC 
ACTATTCGGG ATAAGCGTTC AAATTCCTCG TGGTTGGAGG AATTCTCCAT GGAGCGAAGC 
AGTGAAAATA CCTACAAGTC CCGTCGCCGG AGTTACCACT CCTCCTTGCG GCTGTCAGCC 
CACCGCCTGA ACTCTGATTC AGGCCACAAG TCTGACACTC ACCGCTCAGG GGGCAGGGAG 
CGGGGACGAT CAAAAGAGCG AAAGCATCTT CTCTCTCCTG ATGTCTCCCG CTGCAATTCA 
GAAGAGCGAG GGACCCAGGC TGACTGGGAG TCCCCAGAGC GCCGTCAATC CAGGTCACCC 
AGTGAGGGCA GGTCACAGAC GCCCAACAGA CAGGGCACAG GTTCCCTAAG TGAGAGCTCC 
ATCCCCTCTG TCTCTGACAC CAGCACCCCA AGAAGAAGTC GTCGGCAGCT CCCACCCGTC 
CCGCCAAAGC CCCGGCCCCT CCTTTCCTAC AGCTCCCTGA TTCGACACGC GGGCAGCATC 
TCTCCACCTG CTGATGGAAG CGAGGAGGGC TCCCCGCTGA CCTCCCAAGC TCTGGAGAGC 
AACAATGCTT GCCTGACCGA GTCTTCCAAC TCTCCGCACC CCCAGCAGAG CCAACATGCC 
TCCCCACAGC GCTACATCTC CGAGCCCTAC TTGGCCCTGC ACGAAGACTC CCACGCCTCA 
GACTGTGGTG AGGAGGAGAC GCTCACTTTC GAAGCAGCCG TGGCTACTAG CCTGGGCCGT 
TCCAACACCA TCGGCTCAGC CCCACCCCTG CGGCATAGCT GGCAGATGCC CAACGGGCAC 
TATCGGCGGC GGAGGCGCGG GGGGCCTGGG CCAGGCATGA TGTGTGGGGC TGTCAACAAC 
CTGCTAAGTG ACACGGAAGA AGATGACAAA TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( NM_001205293.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001192222.1 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 1, mRNA.

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CloneID	OHu11422
Clone ID Related Accession (Same CDS sequence)	NM_001205293.1 , NM_001205293.3
Accession Version	NM_001205293.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Homo sapiens(human)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP230397.1, L27745.1, L29385.1, AB209499.1, AL161734.12 and BC037800.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript, and encodes the longest isoform (1, also known as alpha-1E). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L27745.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG 
CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT 
GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG 
TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC 
AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC 
GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT 
CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG 
GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG 
ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG 
AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA 
AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA 
GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA 
CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC 
AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG 
ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC 
TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC 
AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC 
AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG 
AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA 
GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT 
GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC 
ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT 
ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA 
GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCGATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCCGT GTGGGAGCAG 
CGTACCAGCC AGCTGAGGAA GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAGAGAG 
GAGGCGCCGA CCATGAACCC GCTCAACCCC CTCAACCCGC TCAGCTCCCT CAACCCGCTC 
AATGCCCACC CCAGCCTTTA TCGGCGACCC AGGGCCATTG AGGGCCTGGC CCTGGGCCTG 
GCCCTGGAGA AGTTCGAGGA GGAGCGCATC AGCCGTGGGG GGTCCCTCAA GGGGGATGGA 
GGGGACCGAT CCAGTGCCCT GGACAACCAG AGGACCCCTT TGTCCCTGGG CCAGCGGGAG 
CCACCATGGC TGGCCAGGCC CTGTCATGGA AACTGTGACC CGACTCAGCA GGAGGCAGGG 
GGAGGAGAGG CTGTGGTGAC CTTTGAGGAC CGGGCCAGGC ACAGGCAGAG CCAACGGCGC 
AGCCGGCATC GCCGCGTCAG GACAGAAGGC AAGGAGTCCT CTTCAGCCTC CCGGAGCAGG 
TCTGCCAGCC AGGAACGCAG TCTGGATGAA GCCATGCCCA CTGAAGGGGA GAAGGACCAT 
GAGCTCAGGG GCAACCATGG TGCCAAGGAG CCAACGATCC AAGAAGAGAG AGCCCAGGAT 
TTAAGGAGGA CCAACAGTCT GATGGTGTCC AGAGGCTCCG GGCTGGCAGG AGGCCTTGAT 
GAGGCTGACA CCCCCCTAGT CCTGCCCCAT CCTGAGCTGG AAGTGGGGAA GCACGTGGTG 
CTGACGGAGC AGGAGCCAGA AGGCAGCAGT GAGCAGGCCC TGCTGGGGAA TGTGCAGCTA 
GACATGGGCC GGGTCATCAG CCAGAGCGAG CCTGACCTCT CCTGCATCAC GGCCAACACG 
GACAAGGCCA CCACCGAGAG CACCAGCGTC ACCGTCGCCA TCCCCGACGT GGACCCCTTG 
GTGGACTCAA CCGTGGTGCA CATTAGCAAC AAGACGGATG GGGAAGCCAG TCCCTTGAAG 
GAGGCAGAGA TCAGAGAGGA TGAGGAGGAG GTGGAGAAGA AGAAGCAGAA GAAGGAGAAG 
CGTGAGACAG GCAAAGCCAT GGTGCCCCAC AGCTCAATGT TCATCTTCAG CACCACCAAC 
CCGATCCGGA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATCCTC 
CTGGTGATTG CAGCCAGCAG CATCGCCCTG GCGGCAGAGG ACCCCGTCCT GACCAACTCG 
GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTGTTCA CGGGCGTGTT CACCTTTGAG 
ATGGTTATAA AGATGATAGA CCAAGGCTTG ATCCTGCAGG ATGGGTCCTA CTTCCGAGAC 
TTGTGGAACA TCCTGGACTT TGTGGTGGTC GTTGGCGCAT TGGTGGCCTT TGCTCTGGCG 
AACGCTTTGG GAACCAACAA AGGACGGGAC ATCAAGACCA TCAAGTCTCT GCGGGTGCTC 
CGAGTTCTAA GGCCACTGAA AACCATCAAG CGCTTGCCCA AGCTCAAGGC CGTCTTCGAC 
TGCGTAGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TTGTGTACAA GCTCTTCATG 
TTCATCTTTG CTGTCATCGC AGTTCAGCTC TTCAAGGGAA AGTTCTTTTA TTGCACGGAC 
AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTATG TAGATCATGA GAAAAACAAG 
ATGGAGGTGA AGGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG 
GCCCTGCTGA CCCTCTTCAC CGTCTCCACA GGGGAAGGAT GGCCTCAAGT TCTGCAGCAC 
TCTGTAGATG TGACAGAGGA AGACCGAGGC CCAAGCCGCA GCAACCGCAT GGAGATGTCT 
ATCTTTTATG TAGTCTACTT TGTGGTCTTC CCCTTCTTCT TTGTCAATAT CTTTGTGGCT 
CTCATCATCA TCACCTTCCA GGAGCAAGGG GATAAGATGA TGGAGGAGTG CAGCCTGGAG 
AAGAATGAGA GGGCGTGCAT CGACTTCGCC ATCAGCGCCA AACCTCTCAC CCGCTACATG 
CCGCAGAACA GACACACCTT CCAGTACCGC GTGTGGCACT TTGTGGTGTC TCCGTCCTTT 
GAGTACACCA TTATGGCCAT GATCGCCTTG AATACTGTTG TGCTGATGAT GAAGTATTAT 
TCTGCTCCCT GTACCTATGA GCTGGCCCTG AAGTACCTGA ATATCGCCTT CACCATGGTG 
TTTTCCCTGG AATGTGTCCT GAAGGTCATC GCTTTTGGCT TTTTGAACTA TTTCCGAGAC 
ACCTGGAATA TCTTTGACTT CATCACCGTG ATTGGCAGTA TCACAGAAAT TATCCTGACA 
GACAGCAAGC TGGTGAACAC CAGTGGCTTC AATATGAGCT TTCTGAAGCT CTTCCGAGCT 
GCCCGCCTCA TAAAGCTCCT GCGTCAGGGC TATACCATAC GCATTTTGCT GTGGACCTTT 
GTGCAGTCCT TTAAGGCCCT CCCTTATGTC TGCCTTTTAA TTGCCATGCT TTTCTTCATT 
TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGACGAGGA GAGTCACATC 
AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTAA TGCTACTCTT CAGGAGTGCC 
ACAGGTGAGG CCTGGCAGGA GATTATGCTG TCATGCCTTG GGGAGAAGGG CTGTGAGCCT 
GACACCACCG CACCATCAGG GCAGAACGAG AACGAACGCT GCGGCACCGA TCTGGCCTAC 
GTGTACTTTG TCTCCTTCAT CTTCTTCTGC TCCTTCTTGA TGCTCAACCT GTTTGTGGCC 
GTCATCATGG ACAACTTTGA GTACCTGACT CGGGACTCCT CCATCCTGGG GCCTCACCAC 
TTGGACGAGT TTGTCCGCGT CTGGGCAGAA TATGACCGAG CAGCATGTGG CCGCATCCAT 
TACACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCTC CGCTAGGCCT CGGCAAGAGA 
TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTGATGA ACATGCCAGT AGCTGAGGAC 
ATGACGGTCC ACTTCACCTC CACACTTATG GCTCTGATCC GGACAGCTCT GGACATTAAA 
ATTGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCAG AGCTACAAAA GGAGACCCTA 
GCCATCTGGC CTCACCTATC CCAGAAGATG CTGGATCTGC TTGTGCCCAT GCCCAAAGCC 
TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAGCAG 
AGTAAGGTGA AGAAGCAGAG GCAGCAGCTG GAGGAACAGA AAAATGCCCC CATGTTCCAG 
CGCATGGAGC CTTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCTTAC 
CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGGAGTG GATACCCTTC GATGAGTCCA 
CTCTCTCCCC AGGATATATT CCAGTTGGCT TGTATGGACC CCGCCGATGA CGGACAGTTC 
CAAGAACGGC AGTCTCTGGA GCCAGAGGTT AGTGAATTAA AAAGCGTGCA GCCCTCTAAC 
CATGGCATCT ACCTTCCTTC GGACACCCAG GAGCATGCGG GATCTGGGAG GGCATCTTCT 
ATGCCACGTC TGACTGTGGA TCCCCAGGTG GTGACAGACC CTAGCTCCAT GAGACGTTCA 
TTTTCCACTA TTCGGGATAA GCGTTCAAAT TCCTCGTGGT TGGAGGAATT CTCCATGGAG 
CGAAGCAGTG AAAATACCTA CAAGTCCCGT CGCCGGAGTT ACCACTCCTC CTTGCGGCTG 
TCAGCCCACC GCCTGAACTC TGATTCAGGC CACAAGTCTG ACACTCACCG CTCAGGGGGC 
AGGGAGCGGG GACGATCAAA AGAGCGAAAG CATCTTCTCT CTCCTGATGT CTCCCGCTGC 
AATTCAGAAG AGCGAGGGAC CCAGGCTGAC TGGGAGTCCC CAGAGCGCCG TCAATCCAGG 
TCACCCAGTG AGGGCAGGTC ACAGACGCCC AACAGACAGG GCACAGGTTC CCTAAGTGAG 
AGCTCCATCC CCTCTGTCTC TGACACCAGC ACCCCAAGAA GAAGTCGTCG GCAGCTCCCA 
CCCGTCCCGC CAAAGCCCCG GCCCCTCCTT TCCTACAGCT CCCTGATTCG ACACGCGGGC 
AGCATCTCTC CACCTGCTGA TGGAAGCGAG GAGGGCTCCC CGCTGACCTC CCAAGCTCTG 
GAGAGCAACA ATGCTTGCCT GACCGAGTCT TCCAACTCTC CGCACCCCCA GCAGAGCCAA 
CATGCCTCCC CACAGCGCTA CATCTCCGAG CCCTACTTGG CCCTGCACGA AGACTCCCAC 
GCCTCAGACT GTGGTGAGGA GGAGACGCTC ACTTTCGAAG CAGCCGTGGC TACTAGCCTG 
GGCCGTTCCA ACACCATCGG CTCAGCCCCA CCCCTGCGGC ATAGCTGGCA GATGCCCAAC 
GGGCACTATC GGCGGCGGAG GCGCGGGGGG CCTGGGCCAG GCATGATGTG TGGGGCTGTC 
AACAACCTGC TAAGTGACAC GGAAGAAGAT GACAAATGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192222.1
CDS	196..7137
Translation	MARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFEDRARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTSVTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLEPEVSELKSVQPSNHGIYLPSDTQEHAGSGRASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNNLLSDTEEDDKC

Target ORF information:

RefSeq Version	NM_001205293.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205293.1

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG 
CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT 
GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG 
TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC 
AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC 
GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT 
CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG 
GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG 
ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG 
AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA 
AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA 
GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA 
CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC 
AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG 
ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC 
TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC 
AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC 
AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG 
AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA 
GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT 
GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC 
ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT 
ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA 
GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCGATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCCGT GTGGGAGCAG 
CGTACCAGCC AGCTGAGGAA GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAGAGAG 
GAGGCGCCGA CCATGAACCC GCTCAACCCC CTCAACCCGC TCAGCTCCCT CAACCCGCTC 
AATGCCCACC CCAGCCTTTA TCGGCGACCC AGGGCCATTG AGGGCCTGGC CCTGGGCCTG 
GCCCTGGAGA AGTTCGAGGA GGAGCGCATC AGCCGTGGGG GGTCCCTCAA GGGGGATGGA 
GGGGACCGAT CCAGTGCCCT GGACAACCAG AGGACCCCTT TGTCCCTGGG CCAGCGGGAG 
CCACCATGGC TGGCCAGGCC CTGTCATGGA AACTGTGACC CGACTCAGCA GGAGGCAGGG 
GGAGGAGAGG CTGTGGTGAC CTTTGAGGAC CGGGCCAGGC ACAGGCAGAG CCAACGGCGC 
AGCCGGCATC GCCGCGTCAG GACAGAAGGC AAGGAGTCCT CTTCAGCCTC CCGGAGCAGG 
TCTGCCAGCC AGGAACGCAG TCTGGATGAA GCCATGCCCA CTGAAGGGGA GAAGGACCAT 
GAGCTCAGGG GCAACCATGG TGCCAAGGAG CCAACGATCC AAGAAGAGAG AGCCCAGGAT 
TTAAGGAGGA CCAACAGTCT GATGGTGTCC AGAGGCTCCG GGCTGGCAGG AGGCCTTGAT 
GAGGCTGACA CCCCCCTAGT CCTGCCCCAT CCTGAGCTGG AAGTGGGGAA GCACGTGGTG 
CTGACGGAGC AGGAGCCAGA AGGCAGCAGT GAGCAGGCCC TGCTGGGGAA TGTGCAGCTA 
GACATGGGCC GGGTCATCAG CCAGAGCGAG CCTGACCTCT CCTGCATCAC GGCCAACACG 
GACAAGGCCA CCACCGAGAG CACCAGCGTC ACCGTCGCCA TCCCCGACGT GGACCCCTTG 
GTGGACTCAA CCGTGGTGCA CATTAGCAAC AAGACGGATG GGGAAGCCAG TCCCTTGAAG 
GAGGCAGAGA TCAGAGAGGA TGAGGAGGAG GTGGAGAAGA AGAAGCAGAA GAAGGAGAAG 
CGTGAGACAG GCAAAGCCAT GGTGCCCCAC AGCTCAATGT TCATCTTCAG CACCACCAAC 
CCGATCCGGA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATCCTC 
CTGGTGATTG CAGCCAGCAG CATCGCCCTG GCGGCAGAGG ACCCCGTCCT GACCAACTCG 
GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTGTTCA CGGGCGTGTT CACCTTTGAG 
ATGGTTATAA AGATGATAGA CCAAGGCTTG ATCCTGCAGG ATGGGTCCTA CTTCCGAGAC 
TTGTGGAACA TCCTGGACTT TGTGGTGGTC GTTGGCGCAT TGGTGGCCTT TGCTCTGGCG 
AACGCTTTGG GAACCAACAA AGGACGGGAC ATCAAGACCA TCAAGTCTCT GCGGGTGCTC 
CGAGTTCTAA GGCCACTGAA AACCATCAAG CGCTTGCCCA AGCTCAAGGC CGTCTTCGAC 
TGCGTAGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TTGTGTACAA GCTCTTCATG 
TTCATCTTTG CTGTCATCGC AGTTCAGCTC TTCAAGGGAA AGTTCTTTTA TTGCACGGAC 
AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTATG TAGATCATGA GAAAAACAAG 
ATGGAGGTGA AGGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG 
GCCCTGCTGA CCCTCTTCAC CGTCTCCACA GGGGAAGGAT GGCCTCAAGT TCTGCAGCAC 
TCTGTAGATG TGACAGAGGA AGACCGAGGC CCAAGCCGCA GCAACCGCAT GGAGATGTCT 
ATCTTTTATG TAGTCTACTT TGTGGTCTTC CCCTTCTTCT TTGTCAATAT CTTTGTGGCT 
CTCATCATCA TCACCTTCCA GGAGCAAGGG GATAAGATGA TGGAGGAGTG CAGCCTGGAG 
AAGAATGAGA GGGCGTGCAT CGACTTCGCC ATCAGCGCCA AACCTCTCAC CCGCTACATG 
CCGCAGAACA GACACACCTT CCAGTACCGC GTGTGGCACT TTGTGGTGTC TCCGTCCTTT 
GAGTACACCA TTATGGCCAT GATCGCCTTG AATACTGTTG TGCTGATGAT GAAGTATTAT 
TCTGCTCCCT GTACCTATGA GCTGGCCCTG AAGTACCTGA ATATCGCCTT CACCATGGTG 
TTTTCCCTGG AATGTGTCCT GAAGGTCATC GCTTTTGGCT TTTTGAACTA TTTCCGAGAC 
ACCTGGAATA TCTTTGACTT CATCACCGTG ATTGGCAGTA TCACAGAAAT TATCCTGACA 
GACAGCAAGC TGGTGAACAC CAGTGGCTTC AATATGAGCT TTCTGAAGCT CTTCCGAGCT 
GCCCGCCTCA TAAAGCTCCT GCGTCAGGGC TATACCATAC GCATTTTGCT GTGGACCTTT 
GTGCAGTCCT TTAAGGCCCT CCCTTATGTC TGCCTTTTAA TTGCCATGCT TTTCTTCATT 
TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGACGAGGA GAGTCACATC 
AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTAA TGCTACTCTT CAGGAGTGCC 
ACAGGTGAGG CCTGGCAGGA GATTATGCTG TCATGCCTTG GGGAGAAGGG CTGTGAGCCT 
GACACCACCG CACCATCAGG GCAGAACGAG AACGAACGCT GCGGCACCGA TCTGGCCTAC 
GTGTACTTTG TCTCCTTCAT CTTCTTCTGC TCCTTCTTGA TGCTCAACCT GTTTGTGGCC 
GTCATCATGG ACAACTTTGA GTACCTGACT CGGGACTCCT CCATCCTGGG GCCTCACCAC 
TTGGACGAGT TTGTCCGCGT CTGGGCAGAA TATGACCGAG CAGCATGTGG CCGCATCCAT 
TACACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCTC CGCTAGGCCT CGGCAAGAGA 
TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTGATGA ACATGCCAGT AGCTGAGGAC 
ATGACGGTCC ACTTCACCTC CACACTTATG GCTCTGATCC GGACAGCTCT GGACATTAAA 
ATTGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCAG AGCTACAAAA GGAGACCCTA 
GCCATCTGGC CTCACCTATC CCAGAAGATG CTGGATCTGC TTGTGCCCAT GCCCAAAGCC 
TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAGCAG 
AGTAAGGTGA AGAAGCAGAG GCAGCAGCTG GAGGAACAGA AAAATGCCCC CATGTTCCAG 
CGCATGGAGC CTTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCTTAC 
CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGGAGTG GATACCCTTC GATGAGTCCA 
CTCTCTCCCC AGGATATATT CCAGTTGGCT TGTATGGACC CCGCCGATGA CGGACAGTTC 
CAAGAACGGC AGTCTCTGGA GCCAGAGGTT AGTGAATTAA AAAGCGTGCA GCCCTCTAAC 
CATGGCATCT ACCTTCCTTC GGACACCCAG GAGCATGCGG GATCTGGGAG GGCATCTTCT 
ATGCCACGTC TGACTGTGGA TCCCCAGGTG GTGACAGACC CTAGCTCCAT GAGACGTTCA 
TTTTCCACTA TTCGGGATAA GCGTTCAAAT TCCTCGTGGT TGGAGGAATT CTCCATGGAG 
CGAAGCAGTG AAAATACCTA CAAGTCCCGT CGCCGGAGTT ACCACTCCTC CTTGCGGCTG 
TCAGCCCACC GCCTGAACTC TGATTCAGGC CACAAGTCTG ACACTCACCG CTCAGGGGGC 
AGGGAGCGGG GACGATCAAA AGAGCGAAAG CATCTTCTCT CTCCTGATGT CTCCCGCTGC 
AATTCAGAAG AGCGAGGGAC CCAGGCTGAC TGGGAGTCCC CAGAGCGCCG TCAATCCAGG 
TCACCCAGTG AGGGCAGGTC ACAGACGCCC AACAGACAGG GCACAGGTTC CCTAAGTGAG 
AGCTCCATCC CCTCTGTCTC TGACACCAGC ACCCCAAGAA GAAGTCGTCG GCAGCTCCCA 
CCCGTCCCGC CAAAGCCCCG GCCCCTCCTT TCCTACAGCT CCCTGATTCG ACACGCGGGC 
AGCATCTCTC CACCTGCTGA TGGAAGCGAG GAGGGCTCCC CGCTGACCTC CCAAGCTCTG 
GAGAGCAACA ATGCTTGCCT GACCGAGTCT TCCAACTCTC CGCACCCCCA GCAGAGCCAA 
CATGCCTCCC CACAGCGCTA CATCTCCGAG CCCTACTTGG CCCTGCACGA AGACTCCCAC 
GCCTCAGACT GTGGTGAGGA GGAGACGCTC ACTTTCGAAG CAGCCGTGGC TACTAGCCTG 
GGCCGTTCCA ACACCATCGG CTCAGCCCCA CCCCTGCGGC ATAGCTGGCA GATGCCCAAC 
GGGCACTATC GGCGGCGGAG GCGCGGGGGG CCTGGGCCAG GCATGATGTG TGGGGCTGTC 
AACAACCTGC TAAGTGACAC GGAAGAAGAT GACAAATGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( NM_001205293.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001192222.1 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 1, mRNA.

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CloneID	OHu11422
Clone ID Related Accession (Same CDS sequence)	NM_001205293.1 , NM_001205293.3
Accession Version	NM_001205293.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160059.7, BP230397.1, L27745.2, BP230632.1, AL161734.12 and KF510705.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001205293.2. Transcript Variant: This variant (1) represents the longest transcript, and encodes the longest isoform (1, also known as alpha-1E). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L27745.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000367573.7/ ENSP00000356545.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG 
CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT 
GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG 
TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC 
AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC 
GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT 
CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG 
GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG 
ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG 
AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA 
AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA 
GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA 
CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC 
AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG 
ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC 
TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC 
AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC 
AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG 
AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA 
GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT 
GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC 
ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT 
ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA 
GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCGATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCCGT GTGGGAGCAG 
CGTACCAGCC AGCTGAGGAA GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAGAGAG 
GAGGCGCCGA CCATGAACCC GCTCAACCCC CTCAACCCGC TCAGCTCCCT CAACCCGCTC 
AATGCCCACC CCAGCCTTTA TCGGCGACCC AGGGCCATTG AGGGCCTGGC CCTGGGCCTG 
GCCCTGGAGA AGTTCGAGGA GGAGCGCATC AGCCGTGGGG GGTCCCTCAA GGGGGATGGA 
GGGGACCGAT CCAGTGCCCT GGACAACCAG AGGACCCCTT TGTCCCTGGG CCAGCGGGAG 
CCACCATGGC TGGCCAGGCC CTGTCATGGA AACTGTGACC CGACTCAGCA GGAGGCAGGG 
GGAGGAGAGG CTGTGGTGAC CTTTGAGGAC CGGGCCAGGC ACAGGCAGAG CCAACGGCGC 
AGCCGGCATC GCCGCGTCAG GACAGAAGGC AAGGAGTCCT CTTCAGCCTC CCGGAGCAGG 
TCTGCCAGCC AGGAACGCAG TCTGGATGAA GCCATGCCCA CTGAAGGGGA GAAGGACCAT 
GAGCTCAGGG GCAACCATGG TGCCAAGGAG CCAACGATCC AAGAAGAGAG AGCCCAGGAT 
TTAAGGAGGA CCAACAGTCT GATGGTGTCC AGAGGCTCCG GGCTGGCAGG AGGCCTTGAT 
GAGGCTGACA CCCCCCTAGT CCTGCCCCAT CCTGAGCTGG AAGTGGGGAA GCACGTGGTG 
CTGACGGAGC AGGAGCCAGA AGGCAGCAGT GAGCAGGCCC TGCTGGGGAA TGTGCAGCTA 
GACATGGGCC GGGTCATCAG CCAGAGCGAG CCTGACCTCT CCTGCATCAC GGCCAACACG 
GACAAGGCCA CCACCGAGAG CACCAGCGTC ACCGTCGCCA TCCCCGACGT GGACCCCTTG 
GTGGACTCAA CCGTGGTGCA CATTAGCAAC AAGACGGATG GGGAAGCCAG TCCCTTGAAG 
GAGGCAGAGA TCAGAGAGGA TGAGGAGGAG GTGGAGAAGA AGAAGCAGAA GAAGGAGAAG 
CGTGAGACAG GCAAAGCCAT GGTGCCCCAC AGCTCAATGT TCATCTTCAG CACCACCAAC 
CCGATCCGGA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATCCTC 
CTGGTGATTG CAGCCAGCAG CATCGCCCTG GCGGCAGAGG ACCCCGTCCT GACCAACTCG 
GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTGTTCA CGGGCGTGTT CACCTTTGAG 
ATGGTTATAA AGATGATAGA CCAAGGCTTG ATCCTGCAGG ATGGGTCCTA CTTCCGAGAC 
TTGTGGAACA TCCTGGACTT TGTGGTGGTC GTTGGCGCAT TGGTGGCCTT TGCTCTGGCG 
AACGCTTTGG GAACCAACAA AGGACGGGAC ATCAAGACCA TCAAGTCTCT GCGGGTGCTC 
CGAGTTCTAA GGCCACTGAA AACCATCAAG CGCTTGCCCA AGCTCAAGGC CGTCTTCGAC 
TGCGTAGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TTGTGTACAA GCTCTTCATG 
TTCATCTTTG CTGTCATCGC AGTTCAGCTC TTCAAGGGAA AGTTCTTTTA TTGCACGGAC 
AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTATG TAGATCATGA GAAAAACAAG 
ATGGAGGTGA AGGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG 
GCCCTGCTGA CCCTCTTCAC CGTCTCCACA GGGGAAGGAT GGCCTCAAGT TCTGCAGCAC 
TCTGTAGATG TGACAGAGGA AGACCGAGGC CCAAGCCGCA GCAACCGCAT GGAGATGTCT 
ATCTTTTATG TAGTCTACTT TGTGGTCTTC CCCTTCTTCT TTGTCAATAT CTTTGTGGCT 
CTCATCATCA TCACCTTCCA GGAGCAAGGG GATAAGATGA TGGAGGAGTG CAGCCTGGAG 
AAGAATGAGA GGGCGTGCAT CGACTTCGCC ATCAGCGCCA AACCTCTCAC CCGCTACATG 
CCGCAGAACA GACACACCTT CCAGTACCGC GTGTGGCACT TTGTGGTGTC TCCGTCCTTT 
GAGTACACCA TTATGGCCAT GATCGCCTTG AATACTGTTG TGCTGATGAT GAAGTATTAT 
TCTGCTCCCT GTACCTATGA GCTGGCCCTG AAGTACCTGA ATATCGCCTT CACCATGGTG 
TTTTCCCTGG AATGTGTCCT GAAGGTCATC GCTTTTGGCT TTTTGAACTA TTTCCGAGAC 
ACCTGGAATA TCTTTGACTT CATCACCGTG ATTGGCAGTA TCACAGAAAT TATCCTGACA 
GACAGCAAGC TGGTGAACAC CAGTGGCTTC AATATGAGCT TTCTGAAGCT CTTCCGAGCT 
GCCCGCCTCA TAAAGCTCCT GCGTCAGGGC TATACCATAC GCATTTTGCT GTGGACCTTT 
GTGCAGTCCT TTAAGGCCCT CCCTTATGTC TGCCTTTTAA TTGCCATGCT TTTCTTCATT 
TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGACGAGGA GAGTCACATC 
AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTAA TGCTACTCTT CAGGAGTGCC 
ACAGGTGAGG CCTGGCAGGA GATTATGCTG TCATGCCTTG GGGAGAAGGG CTGTGAGCCT 
GACACCACCG CACCATCAGG GCAGAACGAG AACGAACGCT GCGGCACCGA TCTGGCCTAC 
GTGTACTTTG TCTCCTTCAT CTTCTTCTGC TCCTTCTTGA TGCTCAACCT GTTTGTGGCC 
GTCATCATGG ACAACTTTGA GTACCTGACT CGGGACTCCT CCATCCTGGG GCCTCACCAC 
TTGGACGAGT TTGTCCGCGT CTGGGCAGAA TATGACCGAG CAGCATGTGG CCGCATCCAT 
TACACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCTC CGCTAGGCCT CGGCAAGAGA 
TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTGATGA ACATGCCAGT AGCTGAGGAC 
ATGACGGTCC ACTTCACCTC CACACTTATG GCTCTGATCC GGACAGCTCT GGACATTAAA 
ATTGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCAG AGCTACAAAA GGAGACCCTA 
GCCATCTGGC CTCACCTATC CCAGAAGATG CTGGATCTGC TTGTGCCCAT GCCCAAAGCC 
TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAGCAG 
AGTAAGGTGA AGAAGCAGAG GCAGCAGCTG GAGGAACAGA AAAATGCCCC CATGTTCCAG 
CGCATGGAGC CTTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCTTAC 
CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGGAGTG GATACCCTTC GATGAGTCCA 
CTCTCTCCCC AGGATATATT CCAGTTGGCT TGTATGGACC CCGCCGATGA CGGACAGTTC 
CAAGAACGGC AGTCTCTGGA GCCAGAGGTT AGTGAATTAA AAAGCGTGCA GCCCTCTAAC 
CATGGCATCT ACCTTCCTTC GGACACCCAG GAGCATGCGG GATCTGGGAG GGCATCTTCT 
ATGCCACGTC TGACTGTGGA TCCCCAGGTG GTGACAGACC CTAGCTCCAT GAGACGTTCA 
TTTTCCACTA TTCGGGATAA GCGTTCAAAT TCCTCGTGGT TGGAGGAATT CTCCATGGAG 
CGAAGCAGTG AAAATACCTA CAAGTCCCGT CGCCGGAGTT ACCACTCCTC CTTGCGGCTG 
TCAGCCCACC GCCTGAACTC TGATTCAGGC CACAAGTCTG ACACTCACCG CTCAGGGGGC 
AGGGAGCGGG GACGATCAAA AGAGCGAAAG CATCTTCTCT CTCCTGATGT CTCCCGCTGC 
AATTCAGAAG AGCGAGGGAC CCAGGCTGAC TGGGAGTCCC CAGAGCGCCG TCAATCCAGG 
TCACCCAGTG AGGGCAGGTC ACAGACGCCC AACAGACAGG GCACAGGTTC CCTAAGTGAG 
AGCTCCATCC CCTCTGTCTC TGACACCAGC ACCCCAAGAA GAAGTCGTCG GCAGCTCCCA 
CCCGTCCCGC CAAAGCCCCG GCCCCTCCTT TCCTACAGCT CCCTGATTCG ACACGCGGGC 
AGCATCTCTC CACCTGCTGA TGGAAGCGAG GAGGGCTCCC CGCTGACCTC CCAAGCTCTG 
GAGAGCAACA ATGCTTGCCT GACCGAGTCT TCCAACTCTC CGCACCCCCA GCAGAGCCAA 
CATGCCTCCC CACAGCGCTA CATCTCCGAG CCCTACTTGG CCCTGCACGA AGACTCCCAC 
GCCTCAGACT GTGGTGAGGA GGAGACGCTC ACTTTCGAAG CAGCCGTGGC TACTAGCCTG 
GGCCGTTCCA ACACCATCGG CTCAGCCCCA CCCCTGCGGC ATAGCTGGCA GATGCCCAAC 
GGGCACTATC GGCGGCGGAG GCGCGGGGGG CCTGGGCCAG GCATGATGTG TGGGGCTGTC 
AACAACCTGC TAAGTGACAC GGAAGAAGAT GACAAATGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192222.1
CDS	229..7170
Translation	MARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFEDRARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTSVTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLEPEVSELKSVQPSNHGIYLPSDTQEHAGSGRASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNNLLSDTEEDDKC

Target ORF information:

RefSeq Version	NM_001205293.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205293.3

ORF Insert Sequence:

ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG 
CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG 
AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA 
TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT 
GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG 
TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG 
ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG 
AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC 
AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC 
GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT 
CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG 
GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA 
GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG 
GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG 
ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT 
GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC 
TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA 
GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG 
AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA 
AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA 
GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA 
CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC 
AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG 
ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG 
CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC 
TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC 
AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC 
AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT 
AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG 
AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA 
GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT 
GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT 
GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC 
ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT 
ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA 
GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA 
CCCAACATGC CTTCGATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG 
CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCCGT GTGGGAGCAG 
CGTACCAGCC AGCTGAGGAA GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAGAGAG 
GAGGCGCCGA CCATGAACCC GCTCAACCCC CTCAACCCGC TCAGCTCCCT CAACCCGCTC 
AATGCCCACC CCAGCCTTTA TCGGCGACCC AGGGCCATTG AGGGCCTGGC CCTGGGCCTG 
GCCCTGGAGA AGTTCGAGGA GGAGCGCATC AGCCGTGGGG GGTCCCTCAA GGGGGATGGA 
GGGGACCGAT CCAGTGCCCT GGACAACCAG AGGACCCCTT TGTCCCTGGG CCAGCGGGAG 
CCACCATGGC TGGCCAGGCC CTGTCATGGA AACTGTGACC CGACTCAGCA GGAGGCAGGG 
GGAGGAGAGG CTGTGGTGAC CTTTGAGGAC CGGGCCAGGC ACAGGCAGAG CCAACGGCGC 
AGCCGGCATC GCCGCGTCAG GACAGAAGGC AAGGAGTCCT CTTCAGCCTC CCGGAGCAGG 
TCTGCCAGCC AGGAACGCAG TCTGGATGAA GCCATGCCCA CTGAAGGGGA GAAGGACCAT 
GAGCTCAGGG GCAACCATGG TGCCAAGGAG CCAACGATCC AAGAAGAGAG AGCCCAGGAT 
TTAAGGAGGA CCAACAGTCT GATGGTGTCC AGAGGCTCCG GGCTGGCAGG AGGCCTTGAT 
GAGGCTGACA CCCCCCTAGT CCTGCCCCAT CCTGAGCTGG AAGTGGGGAA GCACGTGGTG 
CTGACGGAGC AGGAGCCAGA AGGCAGCAGT GAGCAGGCCC TGCTGGGGAA TGTGCAGCTA 
GACATGGGCC GGGTCATCAG CCAGAGCGAG CCTGACCTCT CCTGCATCAC GGCCAACACG 
GACAAGGCCA CCACCGAGAG CACCAGCGTC ACCGTCGCCA TCCCCGACGT GGACCCCTTG 
GTGGACTCAA CCGTGGTGCA CATTAGCAAC AAGACGGATG GGGAAGCCAG TCCCTTGAAG 
GAGGCAGAGA TCAGAGAGGA TGAGGAGGAG GTGGAGAAGA AGAAGCAGAA GAAGGAGAAG 
CGTGAGACAG GCAAAGCCAT GGTGCCCCAC AGCTCAATGT TCATCTTCAG CACCACCAAC 
CCGATCCGGA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATCCTC 
CTGGTGATTG CAGCCAGCAG CATCGCCCTG GCGGCAGAGG ACCCCGTCCT GACCAACTCG 
GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTGTTCA CGGGCGTGTT CACCTTTGAG 
ATGGTTATAA AGATGATAGA CCAAGGCTTG ATCCTGCAGG ATGGGTCCTA CTTCCGAGAC 
TTGTGGAACA TCCTGGACTT TGTGGTGGTC GTTGGCGCAT TGGTGGCCTT TGCTCTGGCG 
AACGCTTTGG GAACCAACAA AGGACGGGAC ATCAAGACCA TCAAGTCTCT GCGGGTGCTC 
CGAGTTCTAA GGCCACTGAA AACCATCAAG CGCTTGCCCA AGCTCAAGGC CGTCTTCGAC 
TGCGTAGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TTGTGTACAA GCTCTTCATG 
TTCATCTTTG CTGTCATCGC AGTTCAGCTC TTCAAGGGAA AGTTCTTTTA TTGCACGGAC 
AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTATG TAGATCATGA GAAAAACAAG 
ATGGAGGTGA AGGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG 
GCCCTGCTGA CCCTCTTCAC CGTCTCCACA GGGGAAGGAT GGCCTCAAGT TCTGCAGCAC 
TCTGTAGATG TGACAGAGGA AGACCGAGGC CCAAGCCGCA GCAACCGCAT GGAGATGTCT 
ATCTTTTATG TAGTCTACTT TGTGGTCTTC CCCTTCTTCT TTGTCAATAT CTTTGTGGCT 
CTCATCATCA TCACCTTCCA GGAGCAAGGG GATAAGATGA TGGAGGAGTG CAGCCTGGAG 
AAGAATGAGA GGGCGTGCAT CGACTTCGCC ATCAGCGCCA AACCTCTCAC CCGCTACATG 
CCGCAGAACA GACACACCTT CCAGTACCGC GTGTGGCACT TTGTGGTGTC TCCGTCCTTT 
GAGTACACCA TTATGGCCAT GATCGCCTTG AATACTGTTG TGCTGATGAT GAAGTATTAT 
TCTGCTCCCT GTACCTATGA GCTGGCCCTG AAGTACCTGA ATATCGCCTT CACCATGGTG 
TTTTCCCTGG AATGTGTCCT GAAGGTCATC GCTTTTGGCT TTTTGAACTA TTTCCGAGAC 
ACCTGGAATA TCTTTGACTT CATCACCGTG ATTGGCAGTA TCACAGAAAT TATCCTGACA 
GACAGCAAGC TGGTGAACAC CAGTGGCTTC AATATGAGCT TTCTGAAGCT CTTCCGAGCT 
GCCCGCCTCA TAAAGCTCCT GCGTCAGGGC TATACCATAC GCATTTTGCT GTGGACCTTT 
GTGCAGTCCT TTAAGGCCCT CCCTTATGTC TGCCTTTTAA TTGCCATGCT TTTCTTCATT 
TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGACGAGGA GAGTCACATC 
AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTAA TGCTACTCTT CAGGAGTGCC 
ACAGGTGAGG CCTGGCAGGA GATTATGCTG TCATGCCTTG GGGAGAAGGG CTGTGAGCCT 
GACACCACCG CACCATCAGG GCAGAACGAG AACGAACGCT GCGGCACCGA TCTGGCCTAC 
GTGTACTTTG TCTCCTTCAT CTTCTTCTGC TCCTTCTTGA TGCTCAACCT GTTTGTGGCC 
GTCATCATGG ACAACTTTGA GTACCTGACT CGGGACTCCT CCATCCTGGG GCCTCACCAC 
TTGGACGAGT TTGTCCGCGT CTGGGCAGAA TATGACCGAG CAGCATGTGG CCGCATCCAT 
TACACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCTC CGCTAGGCCT CGGCAAGAGA 
TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTGATGA ACATGCCAGT AGCTGAGGAC 
ATGACGGTCC ACTTCACCTC CACACTTATG GCTCTGATCC GGACAGCTCT GGACATTAAA 
ATTGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCAG AGCTACAAAA GGAGACCCTA 
GCCATCTGGC CTCACCTATC CCAGAAGATG CTGGATCTGC TTGTGCCCAT GCCCAAAGCC 
TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAGCAG 
AGTAAGGTGA AGAAGCAGAG GCAGCAGCTG GAGGAACAGA AAAATGCCCC CATGTTCCAG 
CGCATGGAGC CTTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCTTAC 
CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGGAGTG GATACCCTTC GATGAGTCCA 
CTCTCTCCCC AGGATATATT CCAGTTGGCT TGTATGGACC CCGCCGATGA CGGACAGTTC 
CAAGAACGGC AGTCTCTGGA GCCAGAGGTT AGTGAATTAA AAAGCGTGCA GCCCTCTAAC 
CATGGCATCT ACCTTCCTTC GGACACCCAG GAGCATGCGG GATCTGGGAG GGCATCTTCT 
ATGCCACGTC TGACTGTGGA TCCCCAGGTG GTGACAGACC CTAGCTCCAT GAGACGTTCA 
TTTTCCACTA TTCGGGATAA GCGTTCAAAT TCCTCGTGGT TGGAGGAATT CTCCATGGAG 
CGAAGCAGTG AAAATACCTA CAAGTCCCGT CGCCGGAGTT ACCACTCCTC CTTGCGGCTG 
TCAGCCCACC GCCTGAACTC TGATTCAGGC CACAAGTCTG ACACTCACCG CTCAGGGGGC 
AGGGAGCGGG GACGATCAAA AGAGCGAAAG CATCTTCTCT CTCCTGATGT CTCCCGCTGC 
AATTCAGAAG AGCGAGGGAC CCAGGCTGAC TGGGAGTCCC CAGAGCGCCG TCAATCCAGG 
TCACCCAGTG AGGGCAGGTC ACAGACGCCC AACAGACAGG GCACAGGTTC CCTAAGTGAG 
AGCTCCATCC CCTCTGTCTC TGACACCAGC ACCCCAAGAA GAAGTCGTCG GCAGCTCCCA 
CCCGTCCCGC CAAAGCCCCG GCCCCTCCTT TCCTACAGCT CCCTGATTCG ACACGCGGGC 
AGCATCTCTC CACCTGCTGA TGGAAGCGAG GAGGGCTCCC CGCTGACCTC CCAAGCTCTG 
GAGAGCAACA ATGCTTGCCT GACCGAGTCT TCCAACTCTC CGCACCCCCA GCAGAGCCAA 
CATGCCTCCC CACAGCGCTA CATCTCCGAG CCCTACTTGG CCCTGCACGA AGACTCCCAC 
GCCTCAGACT GTGGTGAGGA GGAGACGCTC ACTTTCGAAG CAGCCGTGGC TACTAGCCTG 
GGCCGTTCCA ACACCATCGG CTCAGCCCCA CCCCTGCGGC ATAGCTGGCA GATGCCCAAC 
GGGCACTATC GGCGGCGGAG GCGCGGGGGG CCTGGGCCAG GCATGATGTG TGGGGCTGTC 
AACAACCTGC TAAGTGACAC GGAAGAAGAT GACAAATGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_017002251.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857740.1 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X9, mRNA.

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CloneID	OHu90473
Clone ID Related Accession (Same CDS sequence)	XM_017002251.1 , XM_017002243.1
Accession Version	XM_017002251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC 
ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG 
TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG 
GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC 
TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC 
CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC 
CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC 
CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT 
CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG 
CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA 
GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA 
GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA 
GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG 
GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG 
GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG 
GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC 
ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC 
GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA 
GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG 
CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC 
TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT 
GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC 
GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC 
GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG 
TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG 
GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG 
TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC 
AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG 
TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC 
TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT 
CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC 
GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT 
CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC 
CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC 
AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG 
GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT 
CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG 
GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG 
ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC 
GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG 
AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA 
GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG 
AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT 
TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GCCCTCCCTT ATGTCTGCCT TTTAATTGCC 
ATGCTTTTCT TCATTTATGC CATCATTGGG ATGCAGGTAT TTGGAAACAT AAAATTAGAC 
GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGGTC CCTAATGCTA 
CTCTTCAGGA GTGCCACAGG TGAGGCCTGG CAGGAGATTA TGCTGTCATG CCTTGGGGAG 
AAGGGCTGTG AGCCTGACAC CACCGCACCA TCAGGGCAGA ACGAGAACGA ACGCTGCGGC 
ACCGATCTGG CCTACGTGTA CTTTGTCTCC TTCATCTTCT TCTGCTCCTT CTTGATGCTC 
AACCTGTTTG TGGCCGTCAT CATGGACAAC TTTGAGTACC TGACTCGGGA CTCCTCCATC 
CTGGGGCCTC ACCACTTGGA CGAGTTTGTC CGCGTCTGGG CAGAATATGA CCGAGCAGCA 
TGTGGCCGCA TCCATTACAC TGAGATGTAT GAAATGCTGA CTCTCATGTC ACCTCCGCTA 
GGCCTCGGCA AGAGATGTCC CTCCAAAGTG GCATATAAGA GGTTGGTCCT GATGAACATG 
CCAGTAGCTG AGGACATGAC GGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA 
GCTCTGGACA TTAAAATTGC CAAAGGTGGT GCAGACAGGC AGCAGCTAGA CTCAGAGCTA 
CAAAAGGAGA CCCTAGCCAT CTGGCCTCAC CTATCCCAGA AGATGCTGGA TCTGCTTGTG 
CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAAATCT ATGCAGCAAT GATGATCATG 
GACTACTATA AGCAGAGTAA GGTGAAGAAG CAGAGGCAGC AGCTGGAGGA ACAGAAAAAT 
GCCCCCATGT TCCAGCGCAT GGAGCCTTCA TCTCTGCCTC AGGAGATCAT TGCTAATGCC 
AAAGCCCTGC CTTACCTCCA GCAGGACCCC GTTTCAGGCC TGAGTGGCCG GAGTGGATAC 
CCTTCGATGA GTCCACTCTC TCCCCAGGAT ATATTCCAGT TGGCTTGTAT GGACCCCGCC 
GATGACGGAC AGTTCCAAGA ACGGCAGTCT CTGGAGCCAG AGGTTAGTGA ATTAAAAAGC 
GTGCAGCCCT CTAACCATGG CATCTACCTT CCTTCGGACA CCCAGGAGCA TGCGGGATCT 
GGGAGGGCAT CTTCTATGCC ACGTCTGACT GTGGATCCCC AGGTGGTGAC AGACCCTAGC 
TCCATGAGAC GTTCATTTTC CACTATTCGG GATAAGCGTT CAAATTCCTC GTGGTTGGAG 
GAATTCTCCA TGGAGCGAAG CAGTGAAAAT ACCTACAAGT CCCGTCGCCG GAGTTACCAC 
TCCTCCTTGC GGCTGTCAGC CCACCGCCTG AACTCTGATT CAGGCCACAA GTCTGACACT 
CACCGCTCAG GGGGCAGGGA GCGGGGACGA TCAAAAGAGC GAAAGCATCT TCTCTCTCCT 
GATGTCTCCC GCTGCAATTC AGAAGAGCGA GGGACCCAGG CTGACTGGGA GTCCCCAGAG 
CGCCGTCAAT CCAGGTCACC CAGTGAGGGC AGGTCACAGA CGCCCAACAG ACAGGGCACA 
GGTTCCCTAA GTGAGAGCTC CATCCCCTCT GTCTCTGACA CCAGCACCCC AAGAAGAAGT 
CGTCGGCAGC TCCCACCCGT CCCGCCAAAG CCCCGGCCCC TCCTTTCCTA CAGCTCCCTG 
ATTCGACACG CGGGCAGCAT CTCTCCACCT GCTGATGGAA GCGAGGAGGG CTCCCCGCTG 
ACCTCCCAAG CTCTGGAGAG CAACAATGCT TGCCTGACCG AGTCTTCCAA CTCTCCGCAC 
CCCCAGCAGA GCCAACATGC CTCCCCACAG CGCTACATCT CCGAGCCCTA CTTGGCCCTG 
CACGAAGACT CCCACGCCTC AGACTGTGGT GAGGAGGAGA CGCTCACTTT CGAAGCAGCC 
GTGGCTACTA GCCTGGGCCG TTCCAACACC ATCGGCTCAG CCCCACCCCT GCGGCATAGC 
TGGCAGATGC CCAACGGGCA CTATCGGCGG CGGAGGCGCG GGGGGCCTGG GCCAGGCATG 
ATGTGTGGGG CTGTCAACAA CCTGCTAAGT GACACGGAAG AAGATGACAA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857740.1
CDS	84..7460
Translation	MVPEMRGLSPDRRLPEPAPCPAAGSPEPRSAGRRQRHVLALPEEEAWRPQQCALLEADASDEVGMLPASPRAASGFDNFFQVQEGEGQGWEGAMALEAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFEDRARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTSVTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLEPEVSELKSVQPSNHGIYLPSDTQEHAGSGRASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNNLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017002251.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002251.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC 
ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG 
TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG 
GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC 
TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC 
CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC 
CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC 
CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT 
CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG 
CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA 
GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA 
GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA 
GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG 
GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG 
GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG 
GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC 
ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC 
GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA 
GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG 
CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC 
TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT 
GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC 
GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC 
GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG 
TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG 
GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG 
TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC 
AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG 
TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC 
TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT 
CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC 
GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT 
CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC 
CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC 
AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG 
GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT 
CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG 
GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG 
ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC 
GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG 
AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA 
GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG 
AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT 
TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GCCCTCCCTT ATGTCTGCCT TTTAATTGCC 
ATGCTTTTCT TCATTTATGC CATCATTGGG ATGCAGGTAT TTGGAAACAT AAAATTAGAC 
GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGGTC CCTAATGCTA 
CTCTTCAGGA GTGCCACAGG TGAGGCCTGG CAGGAGATTA TGCTGTCATG CCTTGGGGAG 
AAGGGCTGTG AGCCTGACAC CACCGCACCA TCAGGGCAGA ACGAGAACGA ACGCTGCGGC 
ACCGATCTGG CCTACGTGTA CTTTGTCTCC TTCATCTTCT TCTGCTCCTT CTTGATGCTC 
AACCTGTTTG TGGCCGTCAT CATGGACAAC TTTGAGTACC TGACTCGGGA CTCCTCCATC 
CTGGGGCCTC ACCACTTGGA CGAGTTTGTC CGCGTCTGGG CAGAATATGA CCGAGCAGCA 
TGTGGCCGCA TCCATTACAC TGAGATGTAT GAAATGCTGA CTCTCATGTC ACCTCCGCTA 
GGCCTCGGCA AGAGATGTCC CTCCAAAGTG GCATATAAGA GGTTGGTCCT GATGAACATG 
CCAGTAGCTG AGGACATGAC GGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA 
GCTCTGGACA TTAAAATTGC CAAAGGTGGT GCAGACAGGC AGCAGCTAGA CTCAGAGCTA 
CAAAAGGAGA CCCTAGCCAT CTGGCCTCAC CTATCCCAGA AGATGCTGGA TCTGCTTGTG 
CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAAATCT ATGCAGCAAT GATGATCATG 
GACTACTATA AGCAGAGTAA GGTGAAGAAG CAGAGGCAGC AGCTGGAGGA ACAGAAAAAT 
GCCCCCATGT TCCAGCGCAT GGAGCCTTCA TCTCTGCCTC AGGAGATCAT TGCTAATGCC 
AAAGCCCTGC CTTACCTCCA GCAGGACCCC GTTTCAGGCC TGAGTGGCCG GAGTGGATAC 
CCTTCGATGA GTCCACTCTC TCCCCAGGAT ATATTCCAGT TGGCTTGTAT GGACCCCGCC 
GATGACGGAC AGTTCCAAGA ACGGCAGTCT CTGGAGCCAG AGGTTAGTGA ATTAAAAAGC 
GTGCAGCCCT CTAACCATGG CATCTACCTT CCTTCGGACA CCCAGGAGCA TGCGGGATCT 
GGGAGGGCAT CTTCTATGCC ACGTCTGACT GTGGATCCCC AGGTGGTGAC AGACCCTAGC 
TCCATGAGAC GTTCATTTTC CACTATTCGG GATAAGCGTT CAAATTCCTC GTGGTTGGAG 
GAATTCTCCA TGGAGCGAAG CAGTGAAAAT ACCTACAAGT CCCGTCGCCG GAGTTACCAC 
TCCTCCTTGC GGCTGTCAGC CCACCGCCTG AACTCTGATT CAGGCCACAA GTCTGACACT 
CACCGCTCAG GGGGCAGGGA GCGGGGACGA TCAAAAGAGC GAAAGCATCT TCTCTCTCCT 
GATGTCTCCC GCTGCAATTC AGAAGAGCGA GGGACCCAGG CTGACTGGGA GTCCCCAGAG 
CGCCGTCAAT CCAGGTCACC CAGTGAGGGC AGGTCACAGA CGCCCAACAG ACAGGGCACA 
GGTTCCCTAA GTGAGAGCTC CATCCCCTCT GTCTCTGACA CCAGCACCCC AAGAAGAAGT 
CGTCGGCAGC TCCCACCCGT CCCGCCAAAG CCCCGGCCCC TCCTTTCCTA CAGCTCCCTG 
ATTCGACACG CGGGCAGCAT CTCTCCACCT GCTGATGGAA GCGAGGAGGG CTCCCCGCTG 
ACCTCCCAAG CTCTGGAGAG CAACAATGCT TGCCTGACCG AGTCTTCCAA CTCTCCGCAC 
CCCCAGCAGA GCCAACATGC CTCCCCACAG CGCTACATCT CCGAGCCCTA CTTGGCCCTG 
CACGAAGACT CCCACGCCTC AGACTGTGGT GAGGAGGAGA CGCTCACTTT CGAAGCAGCC 
GTGGCTACTA GCCTGGGCCG TTCCAACACC ATCGGCTCAG CCCCACCCCT GCGGCATAGC 
TGGCAGATGC CCAACGGGCA CTATCGGCGG CGGAGGCGCG GGGGGCCTGG GCCAGGCATG 
ATGTGTGGGG CTGTCAACAA CCTGCTAAGT GACACGGAAG AAGATGACAA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_017002243.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857732.1 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X1, mRNA.

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CloneID	OHu90473
Clone ID Related Accession (Same CDS sequence)	XM_017002251.1 , XM_017002243.1
Accession Version	XM_017002243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC 
ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG 
TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG 
GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC 
TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC 
CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC 
CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC 
CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT 
CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG 
CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA 
GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA 
GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA 
GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG 
GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG 
GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG 
GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC 
ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC 
GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA 
GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG 
CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC 
TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT 
GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC 
GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC 
GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG 
TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG 
GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG 
TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC 
AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG 
TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC 
TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT 
CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC 
GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT 
CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC 
CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC 
AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG 
GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT 
CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG 
GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG 
ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC 
GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG 
AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA 
GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG 
AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT 
TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GCCCTCCCTT ATGTCTGCCT TTTAATTGCC 
ATGCTTTTCT TCATTTATGC CATCATTGGG ATGCAGGTAT TTGGAAACAT AAAATTAGAC 
GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGGTC CCTAATGCTA 
CTCTTCAGGA GTGCCACAGG TGAGGCCTGG CAGGAGATTA TGCTGTCATG CCTTGGGGAG 
AAGGGCTGTG AGCCTGACAC CACCGCACCA TCAGGGCAGA ACGAGAACGA ACGCTGCGGC 
ACCGATCTGG CCTACGTGTA CTTTGTCTCC TTCATCTTCT TCTGCTCCTT CTTGATGCTC 
AACCTGTTTG TGGCCGTCAT CATGGACAAC TTTGAGTACC TGACTCGGGA CTCCTCCATC 
CTGGGGCCTC ACCACTTGGA CGAGTTTGTC CGCGTCTGGG CAGAATATGA CCGAGCAGCA 
TGTGGCCGCA TCCATTACAC TGAGATGTAT GAAATGCTGA CTCTCATGTC ACCTCCGCTA 
GGCCTCGGCA AGAGATGTCC CTCCAAAGTG GCATATAAGA GGTTGGTCCT GATGAACATG 
CCAGTAGCTG AGGACATGAC GGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA 
GCTCTGGACA TTAAAATTGC CAAAGGTGGT GCAGACAGGC AGCAGCTAGA CTCAGAGCTA 
CAAAAGGAGA CCCTAGCCAT CTGGCCTCAC CTATCCCAGA AGATGCTGGA TCTGCTTGTG 
CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAAATCT ATGCAGCAAT GATGATCATG 
GACTACTATA AGCAGAGTAA GGTGAAGAAG CAGAGGCAGC AGCTGGAGGA ACAGAAAAAT 
GCCCCCATGT TCCAGCGCAT GGAGCCTTCA TCTCTGCCTC AGGAGATCAT TGCTAATGCC 
AAAGCCCTGC CTTACCTCCA GCAGGACCCC GTTTCAGGCC TGAGTGGCCG GAGTGGATAC 
CCTTCGATGA GTCCACTCTC TCCCCAGGAT ATATTCCAGT TGGCTTGTAT GGACCCCGCC 
GATGACGGAC AGTTCCAAGA ACGGCAGTCT CTGGAGCCAG AGGTTAGTGA ATTAAAAAGC 
GTGCAGCCCT CTAACCATGG CATCTACCTT CCTTCGGACA CCCAGGAGCA TGCGGGATCT 
GGGAGGGCAT CTTCTATGCC ACGTCTGACT GTGGATCCCC AGGTGGTGAC AGACCCTAGC 
TCCATGAGAC GTTCATTTTC CACTATTCGG GATAAGCGTT CAAATTCCTC GTGGTTGGAG 
GAATTCTCCA TGGAGCGAAG CAGTGAAAAT ACCTACAAGT CCCGTCGCCG GAGTTACCAC 
TCCTCCTTGC GGCTGTCAGC CCACCGCCTG AACTCTGATT CAGGCCACAA GTCTGACACT 
CACCGCTCAG GGGGCAGGGA GCGGGGACGA TCAAAAGAGC GAAAGCATCT TCTCTCTCCT 
GATGTCTCCC GCTGCAATTC AGAAGAGCGA GGGACCCAGG CTGACTGGGA GTCCCCAGAG 
CGCCGTCAAT CCAGGTCACC CAGTGAGGGC AGGTCACAGA CGCCCAACAG ACAGGGCACA 
GGTTCCCTAA GTGAGAGCTC CATCCCCTCT GTCTCTGACA CCAGCACCCC AAGAAGAAGT 
CGTCGGCAGC TCCCACCCGT CCCGCCAAAG CCCCGGCCCC TCCTTTCCTA CAGCTCCCTG 
ATTCGACACG CGGGCAGCAT CTCTCCACCT GCTGATGGAA GCGAGGAGGG CTCCCCGCTG 
ACCTCCCAAG CTCTGGAGAG CAACAATGCT TGCCTGACCG AGTCTTCCAA CTCTCCGCAC 
CCCCAGCAGA GCCAACATGC CTCCCCACAG CGCTACATCT CCGAGCCCTA CTTGGCCCTG 
CACGAAGACT CCCACGCCTC AGACTGTGGT GAGGAGGAGA CGCTCACTTT CGAAGCAGCC 
GTGGCTACTA GCCTGGGCCG TTCCAACACC ATCGGCTCAG CCCCACCCCT GCGGCATAGC 
TGGCAGATGC CCAACGGGCA CTATCGGCGG CGGAGGCGCG GGGGGCCTGG GCCAGGCATG 
ATGTGTGGGG CTGTCAACAA CCTGCTAAGT GACACGGAAG AAGATGACAA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857732.1
CDS	426..7802
Translation	MVPEMRGLSPDRRLPEPAPCPAAGSPEPRSAGRRQRHVLALPEEEAWRPQQCALLEADASDEVGMLPASPRAASGFDNFFQVQEGEGQGWEGAMALEAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFEDRARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTSVTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLEPEVSELKSVQPSNHGIYLPSDTQEHAGSGRASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNNLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017002243.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002243.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC 
ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG 
TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG 
GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC 
TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC 
CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC 
CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC 
CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT 
CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG 
CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA 
GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA 
GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA 
GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG 
GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG 
GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG 
GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC 
ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC 
GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA 
GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG 
CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC 
TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT 
GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC 
GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC 
GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG 
TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG 
GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG 
TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC 
AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG 
TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC 
TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT 
CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC 
GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT 
CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC 
CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC 
AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG 
GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT 
CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG 
GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG 
ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC 
GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG 
AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA 
GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG 
AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT 
TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GCCCTCCCTT ATGTCTGCCT TTTAATTGCC 
ATGCTTTTCT TCATTTATGC CATCATTGGG ATGCAGGTAT TTGGAAACAT AAAATTAGAC 
GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGGTC CCTAATGCTA 
CTCTTCAGGA GTGCCACAGG TGAGGCCTGG CAGGAGATTA TGCTGTCATG CCTTGGGGAG 
AAGGGCTGTG AGCCTGACAC CACCGCACCA TCAGGGCAGA ACGAGAACGA ACGCTGCGGC 
ACCGATCTGG CCTACGTGTA CTTTGTCTCC TTCATCTTCT TCTGCTCCTT CTTGATGCTC 
AACCTGTTTG TGGCCGTCAT CATGGACAAC TTTGAGTACC TGACTCGGGA CTCCTCCATC 
CTGGGGCCTC ACCACTTGGA CGAGTTTGTC CGCGTCTGGG CAGAATATGA CCGAGCAGCA 
TGTGGCCGCA TCCATTACAC TGAGATGTAT GAAATGCTGA CTCTCATGTC ACCTCCGCTA 
GGCCTCGGCA AGAGATGTCC CTCCAAAGTG GCATATAAGA GGTTGGTCCT GATGAACATG 
CCAGTAGCTG AGGACATGAC GGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA 
GCTCTGGACA TTAAAATTGC CAAAGGTGGT GCAGACAGGC AGCAGCTAGA CTCAGAGCTA 
CAAAAGGAGA CCCTAGCCAT CTGGCCTCAC CTATCCCAGA AGATGCTGGA TCTGCTTGTG 
CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAAATCT ATGCAGCAAT GATGATCATG 
GACTACTATA AGCAGAGTAA GGTGAAGAAG CAGAGGCAGC AGCTGGAGGA ACAGAAAAAT 
GCCCCCATGT TCCAGCGCAT GGAGCCTTCA TCTCTGCCTC AGGAGATCAT TGCTAATGCC 
AAAGCCCTGC CTTACCTCCA GCAGGACCCC GTTTCAGGCC TGAGTGGCCG GAGTGGATAC 
CCTTCGATGA GTCCACTCTC TCCCCAGGAT ATATTCCAGT TGGCTTGTAT GGACCCCGCC 
GATGACGGAC AGTTCCAAGA ACGGCAGTCT CTGGAGCCAG AGGTTAGTGA ATTAAAAAGC 
GTGCAGCCCT CTAACCATGG CATCTACCTT CCTTCGGACA CCCAGGAGCA TGCGGGATCT 
GGGAGGGCAT CTTCTATGCC ACGTCTGACT GTGGATCCCC AGGTGGTGAC AGACCCTAGC 
TCCATGAGAC GTTCATTTTC CACTATTCGG GATAAGCGTT CAAATTCCTC GTGGTTGGAG 
GAATTCTCCA TGGAGCGAAG CAGTGAAAAT ACCTACAAGT CCCGTCGCCG GAGTTACCAC 
TCCTCCTTGC GGCTGTCAGC CCACCGCCTG AACTCTGATT CAGGCCACAA GTCTGACACT 
CACCGCTCAG GGGGCAGGGA GCGGGGACGA TCAAAAGAGC GAAAGCATCT TCTCTCTCCT 
GATGTCTCCC GCTGCAATTC AGAAGAGCGA GGGACCCAGG CTGACTGGGA GTCCCCAGAG 
CGCCGTCAAT CCAGGTCACC CAGTGAGGGC AGGTCACAGA CGCCCAACAG ACAGGGCACA 
GGTTCCCTAA GTGAGAGCTC CATCCCCTCT GTCTCTGACA CCAGCACCCC AAGAAGAAGT 
CGTCGGCAGC TCCCACCCGT CCCGCCAAAG CCCCGGCCCC TCCTTTCCTA CAGCTCCCTG 
ATTCGACACG CGGGCAGCAT CTCTCCACCT GCTGATGGAA GCGAGGAGGG CTCCCCGCTG 
ACCTCCCAAG CTCTGGAGAG CAACAATGCT TGCCTGACCG AGTCTTCCAA CTCTCCGCAC 
CCCCAGCAGA GCCAACATGC CTCCCCACAG CGCTACATCT CCGAGCCCTA CTTGGCCCTG 
CACGAAGACT CCCACGCCTC AGACTGTGGT GAGGAGGAGA CGCTCACTTT CGAAGCAGCC 
GTGGCTACTA GCCTGGGCCG TTCCAACACC ATCGGCTCAG CCCCACCCCT GCGGCATAGC 
TGGCAGATGC CCAACGGGCA CTATCGGCGG CGGAGGCGCG GGGGGCCTGG GCCAGGCATG 
ATGTGTGGGG CTGTCAACAA CCTGCTAAGT GACACGGAAG AAGATGACAA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_017002244.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857733.1 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X2, mRNA.

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CloneID	OHu90474
Clone ID Related Accession (Same CDS sequence)	XM_017002244.1
Accession Version	XM_017002244.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC 
ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG 
TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG 
GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC 
TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC 
CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC 
CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC 
CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT 
CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG 
CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA 
GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA 
GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA 
GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG 
GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG 
GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG 
GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC 
ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC 
GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA 
GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG 
CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC 
TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT 
GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC 
GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC 
GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG 
TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG 
GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG 
TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC 
AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG 
TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC 
TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT 
CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC 
GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT 
CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC 
CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC 
AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG 
GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT 
CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG 
GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG 
ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC 
GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG 
AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA 
GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG 
AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT 
TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GCCCTCCCTT ATGTCTGCCT TTTAATTGCC 
ATGCTTTTCT TCATTTATGC CATCATTGGG ATGCAGGTAT TTGGAAACAT AAAATTAGAC 
GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGGTC CCTAATGCTA 
CTCTTCAGGA GTGCCACAGG TGAGGCCTGG CAGGAGATTA TGCTGTCATG CCTTGGGGAG 
AAGGGCTGTG AGCCTGACAC CACCGCACCA TCAGGGCAGA ACGAGAACGA ACGCTGCGGC 
ACCGATCTGG CCTACGTGTA CTTTGTCTCC TTCATCTTCT TCTGCTCCTT CTTGATGCTC 
AACCTGTTTG TGGCCGTCAT CATGGACAAC TTTGAGTACC TGACTCGGGA CTCCTCCATC 
CTGGGGCCTC ACCACTTGGA CGAGTTTGTC CGCGTCTGGG CAGAATATGA CCGAGCAGCA 
TGCGGCAGGA TCCCGTATAA GGATATGTAC AAATTAGTGC GGGTGATCTC GCCTCCTCTG 
GGCCTGGGAG AGAACTGCCC CTACAGGGTG GCTTGCAAGA GGTTGGTCCT GATGAACATG 
CCAGTAGCTG AGGACATGAC GGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA 
GCTCTGGACA TTAAAATTGC CAAAGGTGGT GCAGACAGGC AGCAGCTAGA CTCAGAGCTA 
CAAAAGGAGA CCCTAGCCAT CTGGCCTCAC CTATCCCAGA AGATGCTGGA TCTGCTTGTG 
CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAAATCT ATGCAGCAAT GATGATCATG 
GACTACTATA AGCAGAGTAA GGTGAAGAAG CAGAGGCAGC AGCTGGAGGA ACAGAAAAAT 
GCCCCCATGT TCCAGCGCAT GGAGCCTTCA TCTCTGCCTC AGGAGATCAT TGCTAATGCC 
AAAGCCCTGC CTTACCTCCA GCAGGACCCC GTTTCAGGCC TGAGTGGCCG GAGTGGATAC 
CCTTCGATGA GTCCACTCTC TCCCCAGGAT ATATTCCAGT TGGCTTGTAT GGACCCCGCC 
GATGACGGAC AGTTCCAAGA ACGGCAGTCT CTGGAGCCAG AGGTTAGTGA ATTAAAAAGC 
GTGCAGCCCT CTAACCATGG CATCTACCTT CCTTCGGACA CCCAGGAGCA TGCGGGATCT 
GGGAGGGCAT CTTCTATGCC ACGTCTGACT GTGGATCCCC AGGTGGTGAC AGACCCTAGC 
TCCATGAGAC GTTCATTTTC CACTATTCGG GATAAGCGTT CAAATTCCTC GTGGTTGGAG 
GAATTCTCCA TGGAGCGAAG CAGTGAAAAT ACCTACAAGT CCCGTCGCCG GAGTTACCAC 
TCCTCCTTGC GGCTGTCAGC CCACCGCCTG AACTCTGATT CAGGCCACAA GTCTGACACT 
CACCGCTCAG GGGGCAGGGA GCGGGGACGA TCAAAAGAGC GAAAGCATCT TCTCTCTCCT 
GATGTCTCCC GCTGCAATTC AGAAGAGCGA GGGACCCAGG CTGACTGGGA GTCCCCAGAG 
CGCCGTCAAT CCAGGTCACC CAGTGAGGGC AGGTCACAGA CGCCCAACAG ACAGGGCACA 
GGTTCCCTAA GTGAGAGCTC CATCCCCTCT GTCTCTGACA CCAGCACCCC AAGAAGAAGT 
CGTCGGCAGC TCCCACCCGT CCCGCCAAAG CCCCGGCCCC TCCTTTCCTA CAGCTCCCTG 
ATTCGACACG CGGGCAGCAT CTCTCCACCT GCTGATGGAA GCGAGGAGGG CTCCCCGCTG 
ACCTCCCAAG CTCTGGAGAG CAACAATGCT TGCCTGACCG AGTCTTCCAA CTCTCCGCAC 
CCCCAGCAGA GCCAACATGC CTCCCCACAG CGCTACATCT CCGAGCCCTA CTTGGCCCTG 
CACGAAGACT CCCACGCCTC AGACTGTGGT GAGGAGGAGA CGCTCACTTT CGAAGCAGCC 
GTGGCTACTA GCCTGGGCCG TTCCAACACC ATCGGCTCAG CCCCACCCCT GCGGCATAGC 
TGGCAGATGC CCAACGGGCA CTATCGGCGG CGGAGGCGCG GGGGGCCTGG GCCAGGCATG 
ATGTGTGGGG CTGTCAACAA CCTGCTAAGT GACACGGAAG AAGATGACAA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857733.1
CDS	427..7803
Translation	MVPEMRGLSPDRRLPEPAPCPAAGSPEPRSAGRRQRHVLALPEEEAWRPQQCALLEADASDEVGMLPASPRAASGFDNFFQVQEGEGQGWEGAMALEAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFEDRARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTSVTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLEPEVSELKSVQPSNHGIYLPSDTQEHAGSGRASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNNLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017002244.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002244.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC 
ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG 
TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG 
GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC 
TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC 
CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC 
CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC 
CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT 
CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG 
CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA 
GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA 
GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA 
GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG 
GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG 
GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG 
GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC 
ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC 
GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA 
GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG 
CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC 
TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT 
GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC 
GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC 
GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG 
TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG 
GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG 
TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC 
AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG 
TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC 
TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT 
CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC 
GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT 
CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC 
CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC 
AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG 
GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT 
CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG 
GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG 
ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC 
GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG 
AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA 
GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG 
AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT 
TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GCCCTCCCTT ATGTCTGCCT TTTAATTGCC 
ATGCTTTTCT TCATTTATGC CATCATTGGG ATGCAGGTAT TTGGAAACAT AAAATTAGAC 
GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGGTC CCTAATGCTA 
CTCTTCAGGA GTGCCACAGG TGAGGCCTGG CAGGAGATTA TGCTGTCATG CCTTGGGGAG 
AAGGGCTGTG AGCCTGACAC CACCGCACCA TCAGGGCAGA ACGAGAACGA ACGCTGCGGC 
ACCGATCTGG CCTACGTGTA CTTTGTCTCC TTCATCTTCT TCTGCTCCTT CTTGATGCTC 
AACCTGTTTG TGGCCGTCAT CATGGACAAC TTTGAGTACC TGACTCGGGA CTCCTCCATC 
CTGGGGCCTC ACCACTTGGA CGAGTTTGTC CGCGTCTGGG CAGAATATGA CCGAGCAGCA 
TGCGGCAGGA TCCCGTATAA GGATATGTAC AAATTAGTGC GGGTGATCTC GCCTCCTCTG 
GGCCTGGGAG AGAACTGCCC CTACAGGGTG GCTTGCAAGA GGTTGGTCCT GATGAACATG 
CCAGTAGCTG AGGACATGAC GGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA 
GCTCTGGACA TTAAAATTGC CAAAGGTGGT GCAGACAGGC AGCAGCTAGA CTCAGAGCTA 
CAAAAGGAGA CCCTAGCCAT CTGGCCTCAC CTATCCCAGA AGATGCTGGA TCTGCTTGTG 
CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAAATCT ATGCAGCAAT GATGATCATG 
GACTACTATA AGCAGAGTAA GGTGAAGAAG CAGAGGCAGC AGCTGGAGGA ACAGAAAAAT 
GCCCCCATGT TCCAGCGCAT GGAGCCTTCA TCTCTGCCTC AGGAGATCAT TGCTAATGCC 
AAAGCCCTGC CTTACCTCCA GCAGGACCCC GTTTCAGGCC TGAGTGGCCG GAGTGGATAC 
CCTTCGATGA GTCCACTCTC TCCCCAGGAT ATATTCCAGT TGGCTTGTAT GGACCCCGCC 
GATGACGGAC AGTTCCAAGA ACGGCAGTCT CTGGAGCCAG AGGTTAGTGA ATTAAAAAGC 
GTGCAGCCCT CTAACCATGG CATCTACCTT CCTTCGGACA CCCAGGAGCA TGCGGGATCT 
GGGAGGGCAT CTTCTATGCC ACGTCTGACT GTGGATCCCC AGGTGGTGAC AGACCCTAGC 
TCCATGAGAC GTTCATTTTC CACTATTCGG GATAAGCGTT CAAATTCCTC GTGGTTGGAG 
GAATTCTCCA TGGAGCGAAG CAGTGAAAAT ACCTACAAGT CCCGTCGCCG GAGTTACCAC 
TCCTCCTTGC GGCTGTCAGC CCACCGCCTG AACTCTGATT CAGGCCACAA GTCTGACACT 
CACCGCTCAG GGGGCAGGGA GCGGGGACGA TCAAAAGAGC GAAAGCATCT TCTCTCTCCT 
GATGTCTCCC GCTGCAATTC AGAAGAGCGA GGGACCCAGG CTGACTGGGA GTCCCCAGAG 
CGCCGTCAAT CCAGGTCACC CAGTGAGGGC AGGTCACAGA CGCCCAACAG ACAGGGCACA 
GGTTCCCTAA GTGAGAGCTC CATCCCCTCT GTCTCTGACA CCAGCACCCC AAGAAGAAGT 
CGTCGGCAGC TCCCACCCGT CCCGCCAAAG CCCCGGCCCC TCCTTTCCTA CAGCTCCCTG 
ATTCGACACG CGGGCAGCAT CTCTCCACCT GCTGATGGAA GCGAGGAGGG CTCCCCGCTG 
ACCTCCCAAG CTCTGGAGAG CAACAATGCT TGCCTGACCG AGTCTTCCAA CTCTCCGCAC 
CCCCAGCAGA GCCAACATGC CTCCCCACAG CGCTACATCT CCGAGCCCTA CTTGGCCCTG 
CACGAAGACT CCCACGCCTC AGACTGTGGT GAGGAGGAGA CGCTCACTTT CGAAGCAGCC 
GTGGCTACTA GCCTGGGCCG TTCCAACACC ATCGGCTCAG CCCCACCCCT GCGGCATAGC 
TGGCAGATGC CCAACGGGCA CTATCGGCGG CGGAGGCGCG GGGGGCCTGG GCCAGGCATG 
ATGTGTGGGG CTGTCAACAA CCTGCTAAGT GACACGGAAG AAGATGACAA ATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_017002245.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857734.1 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X3, mRNA.

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CloneID	OHu90475
Clone ID Related Accession (Same CDS sequence)	XM_017002245.1
Accession Version	XM_017002245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC 
ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG 
TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG 
GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC 
TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC 
CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC 
CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC 
CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT 
CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG 
CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA 
GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA 
GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA 
GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG 
GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG 
GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG 
GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC 
ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC 
GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA 
GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG 
CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC 
TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT 
GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC 
GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC 
GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG 
TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG 
GCCTTTGCTC TGGCAACCAA CAAAGGACGG GACATCAAGA CCATCAAGTC TCTGCGGGTG 
CTCCGAGTTC TAAGGCCACT GAAAACCATC AAGCGCTTGC CCAAGCTCAA GGCCGTCTTC 
GACTGCGTAG TGACCTCCTT GAAGAATGTC TTCAACATAC TCATTGTGTA CAAGCTCTTC 
ATGTTCATCT TTGCTGTCAT CGCAGTTCAG CTCTTCAAGG GAAAGTTCTT TTATTGCACG 
GACAGTTCCA AGGACACAGA GAAGGAGTGC ATAGGCAACT ATGTAGATCA TGAGAAAAAC 
AAGATGGAGG TGAAGGGCCG GGAATGGAAG CGCCATGAAT TCCACTACGA CAACATTATC 
TGGGCCCTGC TGACCCTCTT CACCGTCTCC ACAGGGGAAG GATGGCCTCA AGTTCTGCAG 
CACTCTGTAG ATGTGACAGA GGAAGACCGA GGCCCAAGCC GCAGCAACCG CATGGAGATG 
TCTATCTTTT ATGTAGTCTA CTTTGTGGTC TTCCCCTTCT TCTTTGTCAA TATCTTTGTG 
GCTCTCATCA TCATCACCTT CCAGGAGCAA GGGGATAAGA TGATGGAGGA GTGCAGCCTG 
GAGAAGAATG AGAGGGCGTG CATCGACTTC GCCATCAGCG CCAAACCTCT CACCCGCTAC 
ATGCCGCAGA ACAGACACAC CTTCCAGTAC CGCGTGTGGC ACTTTGTGGT GTCTCCGTCC 
TTTGAGTACA CCATTATGGC CATGATCGCC TTGAATACTG TTGTGCTGAT GATGAAGTAT 
TATTCTGCTC CCTGTACCTA TGAGCTGGCC CTGAAGTACC TGAATATCGC CTTCACCATG 
GTGTTTTCCC TGGAATGTGT CCTGAAGGTC ATCGCTTTTG GCTTTTTGAA CTATTTCCGA 
GACACCTGGA ATATCTTTGA CTTCATCACC GTGATTGGCA GTATCACAGA AATTATCCTG 
ACAGACAGCA AGCTGGTGAA CACCAGTGGC TTCAATATGA GCTTTCTGAA GCTCTTCCGA 
GCTGCCCGCC TCATAAAGCT CCTGCGTCAG GGCTATACCA TACGCATTTT GCTGTGGACC 
TTTGTGCAGT CCTTTAAGGC CCTCCCTTAT GTCTGCCTTT TAATTGCCAT GCTTTTCTTC 
ATTTATGCCA TCATTGGGAT GCAGGTATTT GGAAACATAA AATTAGACGA GGAGAGTCAC 
ATCAACCGGC ACAACAACTT CCGGAGTTTC TTTGGGTCCC TAATGCTACT CTTCAGGAGT 
GCCACAGGTG AGGCCTGGCA GGAGATTATG CTGTCATGCC TTGGGGAGAA GGGCTGTGAG 
CCTGACACCA CCGCACCATC AGGGCAGAAC GAGAACGAAC GCTGCGGCAC CGATCTGGCC 
TACGTGTACT TTGTCTCCTT CATCTTCTTC TGCTCCTTCT TGATGCTCAA CCTGTTTGTG 
GCCGTCATCA TGGACAACTT TGAGTACCTG ACTCGGGACT CCTCCATCCT GGGGCCTCAC 
CACTTGGACG AGTTTGTCCG CGTCTGGGCA GAATATGACC GAGCAGCATG TGGCCGCATC 
CATTACACTG AGATGTATGA AATGCTGACT CTCATGTCAC CTCCGCTAGG CCTCGGCAAG 
AGATGTCCCT CCAAAGTGGC ATATAAGAGG TTGGTCCTGA TGAACATGCC AGTAGCTGAG 
GACATGACGG TCCACTTCAC CTCCACACTT ATGGCTCTGA TCCGGACAGC TCTGGACATT 
AAAATTGCCA AAGGTGGTGC AGACAGGCAG CAGCTAGACT CAGAGCTACA AAAGGAGACC 
CTAGCCATCT GGCCTCACCT ATCCCAGAAG ATGCTGGATC TGCTTGTGCC CATGCCCAAA 
GCCTCTGACC TGACTGTGGG CAAAATCTAT GCAGCAATGA TGATCATGGA CTACTATAAG 
CAGAGTAAGG TGAAGAAGCA GAGGCAGCAG CTGGAGGAAC AGAAAAATGC CCCCATGTTC 
CAGCGCATGG AGCCTTCATC TCTGCCTCAG GAGATCATTG CTAATGCCAA AGCCCTGCCT 
TACCTCCAGC AGGACCCCGT TTCAGGCCTG AGTGGCCGGA GTGGATACCC TTCGATGAGT 
CCACTCTCTC CCCAGGATAT ATTCCAGTTG GCTTGTATGG ACCCCGCCGA TGACGGACAG 
TTCCAAGAAC GGCAGTCTCT GGAGCCAGAG GTTAGTGAAT TAAAAAGCGT GCAGCCCTCT 
AACCATGGCA TCTACCTTCC TTCGGACACC CAGGAGCATG CGGGATCTGG GAGGGCATCT 
TCTATGCCAC GTCTGACTGT GGATCCCCAG GTGGTGACAG ACCCTAGCTC CATGAGACGT 
TCATTTTCCA CTATTCGGGA TAAGCGTTCA AATTCCTCGT GGTTGGAGGA ATTCTCCATG 
GAGCGAAGCA GTGAAAATAC CTACAAGTCC CGTCGCCGGA GTTACCACTC CTCCTTGCGG 
CTGTCAGCCC ACCGCCTGAA CTCTGATTCA GGCCACAAGT CTGACACTCA CCGCTCAGGG 
GGCAGGGAGC GGGGACGATC AAAAGAGCGA AAGCATCTTC TCTCTCCTGA TGTCTCCCGC 
TGCAATTCAG AAGAGCGAGG GACCCAGGCT GACTGGGAGT CCCCAGAGCG CCGTCAATCC 
AGGTCACCCA GTGAGGGCAG GTCACAGACG CCCAACAGAC AGGGCACAGG TTCCCTAAGT 
GAGAGCTCCA TCCCCTCTGT CTCTGACACC AGCACCCCAA GAAGAAGTCG TCGGCAGCTC 
CCACCCGTCC CGCCAAAGCC CCGGCCCCTC CTTTCCTACA GCTCCCTGAT TCGACACGCG 
GGCAGCATCT CTCCACCTGC TGATGGAAGC GAGGAGGGCT CCCCGCTGAC CTCCCAAGCT 
CTGGAGAGCA ACAATGCTTG CCTGACCGAG TCTTCCAACT CTCCGCACCC CCAGCAGAGC 
CAACATGCCT CCCCACAGCG CTACATCTCC GAGCCCTACT TGGCCCTGCA CGAAGACTCC 
CACGCCTCAG ACTGTGGTGA GGAGGAGACG CTCACTTTCG AAGCAGCCGT GGCTACTAGC 
CTGGGCCGTT CCAACACCAT CGGCTCAGCC CCACCCCTGC GGCATAGCTG GCAGATGCCC 
AACGGGCACT ATCGGCGGCG GAGGCGCGGG GGGCCTGGGC CAGGCATGAT GTGTGGGGCT 
GTCAACAACC TGCTAAGTGA CACGGAAGAA GATGACAAAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857734.1
CDS	427..7791
Translation	MVPEMRGLSPDRRLPEPAPCPAAGSPEPRSAGRRQRHVLALPEEEAWRPQQCALLEADASDEVGMLPASPRAASGFDNFFQVQEGEGQGWEGAMALEAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFEDRARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTSVTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLEPEVSELKSVQPSNHGIYLPSDTQEHAGSGRASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNNLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017002245.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002245.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC 
ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG 
TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG 
GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC 
TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC 
CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC 
CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC 
CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT 
CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG 
CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA 
GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA 
GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA 
GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG 
GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG 
GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG 
GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC 
ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC 
GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA 
GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG 
CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC 
TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT 
GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC 
GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC 
GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG 
TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG 
GCCTTTGCTC TGGCAACCAA CAAAGGACGG GACATCAAGA CCATCAAGTC TCTGCGGGTG 
CTCCGAGTTC TAAGGCCACT GAAAACCATC AAGCGCTTGC CCAAGCTCAA GGCCGTCTTC 
GACTGCGTAG TGACCTCCTT GAAGAATGTC TTCAACATAC TCATTGTGTA CAAGCTCTTC 
ATGTTCATCT TTGCTGTCAT CGCAGTTCAG CTCTTCAAGG GAAAGTTCTT TTATTGCACG 
GACAGTTCCA AGGACACAGA GAAGGAGTGC ATAGGCAACT ATGTAGATCA TGAGAAAAAC 
AAGATGGAGG TGAAGGGCCG GGAATGGAAG CGCCATGAAT TCCACTACGA CAACATTATC 
TGGGCCCTGC TGACCCTCTT CACCGTCTCC ACAGGGGAAG GATGGCCTCA AGTTCTGCAG 
CACTCTGTAG ATGTGACAGA GGAAGACCGA GGCCCAAGCC GCAGCAACCG CATGGAGATG 
TCTATCTTTT ATGTAGTCTA CTTTGTGGTC TTCCCCTTCT TCTTTGTCAA TATCTTTGTG 
GCTCTCATCA TCATCACCTT CCAGGAGCAA GGGGATAAGA TGATGGAGGA GTGCAGCCTG 
GAGAAGAATG AGAGGGCGTG CATCGACTTC GCCATCAGCG CCAAACCTCT CACCCGCTAC 
ATGCCGCAGA ACAGACACAC CTTCCAGTAC CGCGTGTGGC ACTTTGTGGT GTCTCCGTCC 
TTTGAGTACA CCATTATGGC CATGATCGCC TTGAATACTG TTGTGCTGAT GATGAAGTAT 
TATTCTGCTC CCTGTACCTA TGAGCTGGCC CTGAAGTACC TGAATATCGC CTTCACCATG 
GTGTTTTCCC TGGAATGTGT CCTGAAGGTC ATCGCTTTTG GCTTTTTGAA CTATTTCCGA 
GACACCTGGA ATATCTTTGA CTTCATCACC GTGATTGGCA GTATCACAGA AATTATCCTG 
ACAGACAGCA AGCTGGTGAA CACCAGTGGC TTCAATATGA GCTTTCTGAA GCTCTTCCGA 
GCTGCCCGCC TCATAAAGCT CCTGCGTCAG GGCTATACCA TACGCATTTT GCTGTGGACC 
TTTGTGCAGT CCTTTAAGGC CCTCCCTTAT GTCTGCCTTT TAATTGCCAT GCTTTTCTTC 
ATTTATGCCA TCATTGGGAT GCAGGTATTT GGAAACATAA AATTAGACGA GGAGAGTCAC 
ATCAACCGGC ACAACAACTT CCGGAGTTTC TTTGGGTCCC TAATGCTACT CTTCAGGAGT 
GCCACAGGTG AGGCCTGGCA GGAGATTATG CTGTCATGCC TTGGGGAGAA GGGCTGTGAG 
CCTGACACCA CCGCACCATC AGGGCAGAAC GAGAACGAAC GCTGCGGCAC CGATCTGGCC 
TACGTGTACT TTGTCTCCTT CATCTTCTTC TGCTCCTTCT TGATGCTCAA CCTGTTTGTG 
GCCGTCATCA TGGACAACTT TGAGTACCTG ACTCGGGACT CCTCCATCCT GGGGCCTCAC 
CACTTGGACG AGTTTGTCCG CGTCTGGGCA GAATATGACC GAGCAGCATG TGGCCGCATC 
CATTACACTG AGATGTATGA AATGCTGACT CTCATGTCAC CTCCGCTAGG CCTCGGCAAG 
AGATGTCCCT CCAAAGTGGC ATATAAGAGG TTGGTCCTGA TGAACATGCC AGTAGCTGAG 
GACATGACGG TCCACTTCAC CTCCACACTT ATGGCTCTGA TCCGGACAGC TCTGGACATT 
AAAATTGCCA AAGGTGGTGC AGACAGGCAG CAGCTAGACT CAGAGCTACA AAAGGAGACC 
CTAGCCATCT GGCCTCACCT ATCCCAGAAG ATGCTGGATC TGCTTGTGCC CATGCCCAAA 
GCCTCTGACC TGACTGTGGG CAAAATCTAT GCAGCAATGA TGATCATGGA CTACTATAAG 
CAGAGTAAGG TGAAGAAGCA GAGGCAGCAG CTGGAGGAAC AGAAAAATGC CCCCATGTTC 
CAGCGCATGG AGCCTTCATC TCTGCCTCAG GAGATCATTG CTAATGCCAA AGCCCTGCCT 
TACCTCCAGC AGGACCCCGT TTCAGGCCTG AGTGGCCGGA GTGGATACCC TTCGATGAGT 
CCACTCTCTC CCCAGGATAT ATTCCAGTTG GCTTGTATGG ACCCCGCCGA TGACGGACAG 
TTCCAAGAAC GGCAGTCTCT GGAGCCAGAG GTTAGTGAAT TAAAAAGCGT GCAGCCCTCT 
AACCATGGCA TCTACCTTCC TTCGGACACC CAGGAGCATG CGGGATCTGG GAGGGCATCT 
TCTATGCCAC GTCTGACTGT GGATCCCCAG GTGGTGACAG ACCCTAGCTC CATGAGACGT 
TCATTTTCCA CTATTCGGGA TAAGCGTTCA AATTCCTCGT GGTTGGAGGA ATTCTCCATG 
GAGCGAAGCA GTGAAAATAC CTACAAGTCC CGTCGCCGGA GTTACCACTC CTCCTTGCGG 
CTGTCAGCCC ACCGCCTGAA CTCTGATTCA GGCCACAAGT CTGACACTCA CCGCTCAGGG 
GGCAGGGAGC GGGGACGATC AAAAGAGCGA AAGCATCTTC TCTCTCCTGA TGTCTCCCGC 
TGCAATTCAG AAGAGCGAGG GACCCAGGCT GACTGGGAGT CCCCAGAGCG CCGTCAATCC 
AGGTCACCCA GTGAGGGCAG GTCACAGACG CCCAACAGAC AGGGCACAGG TTCCCTAAGT 
GAGAGCTCCA TCCCCTCTGT CTCTGACACC AGCACCCCAA GAAGAAGTCG TCGGCAGCTC 
CCACCCGTCC CGCCAAAGCC CCGGCCCCTC CTTTCCTACA GCTCCCTGAT TCGACACGCG 
GGCAGCATCT CTCCACCTGC TGATGGAAGC GAGGAGGGCT CCCCGCTGAC CTCCCAAGCT 
CTGGAGAGCA ACAATGCTTG CCTGACCGAG TCTTCCAACT CTCCGCACCC CCAGCAGAGC 
CAACATGCCT CCCCACAGCG CTACATCTCC GAGCCCTACT TGGCCCTGCA CGAAGACTCC 
CACGCCTCAG ACTGTGGTGA GGAGGAGACG CTCACTTTCG AAGCAGCCGT GGCTACTAGC 
CTGGGCCGTT CCAACACCAT CGGCTCAGCC CCACCCCTGC GGCATAGCTG GCAGATGCCC 
AACGGGCACT ATCGGCGGCG GAGGCGCGGG GGGCCTGGGC CAGGCATGAT GTGTGGGGCT 
GTCAACAACC TGCTAAGTGA CACGGAAGAA GATGACAAAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_017002246.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857735.1 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X4, mRNA.

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CloneID	OHu90476
Clone ID Related Accession (Same CDS sequence)	XM_017002246.1
Accession Version	XM_017002246.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC 
ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG 
TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG 
GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC 
TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC 
CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC 
CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC 
CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT 
CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG 
CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA 
GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA 
GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA 
GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG 
GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG 
GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG 
GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC 
ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC 
GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA 
GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG 
CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC 
TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT 
GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC 
GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC 
GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG 
TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG 
GCCTTTGCTC TGGCAACCAA CAAAGGACGG GACATCAAGA CCATCAAGTC TCTGCGGGTG 
CTCCGAGTTC TAAGGCCACT GAAAACCATC AAGCGCTTGC CCAAGCTCAA GGCCGTCTTC 
GACTGCGTAG TGACCTCCTT GAAGAATGTC TTCAACATAC TCATTGTGTA CAAGCTCTTC 
ATGTTCATCT TTGCTGTCAT CGCAGTTCAG CTCTTCAAGG GAAAGTTCTT TTATTGCACG 
GACAGTTCCA AGGACACAGA GAAGGAGTGC ATAGGCAACT ATGTAGATCA TGAGAAAAAC 
AAGATGGAGG TGAAGGGCCG GGAATGGAAG CGCCATGAAT TCCACTACGA CAACATTATC 
TGGGCCCTGC TGACCCTCTT CACCGTCTCC ACAGGGGAAG GATGGCCTCA AGTTCTGCAG 
CACTCTGTAG ATGTGACAGA GGAAGACCGA GGCCCAAGCC GCAGCAACCG CATGGAGATG 
TCTATCTTTT ATGTAGTCTA CTTTGTGGTC TTCCCCTTCT TCTTTGTCAA TATCTTTGTG 
GCTCTCATCA TCATCACCTT CCAGGAGCAA GGGGATAAGA TGATGGAGGA GTGCAGCCTG 
GAGAAGAATG AGAGGGCGTG CATCGACTTC GCCATCAGCG CCAAACCTCT CACCCGCTAC 
ATGCCGCAGA ACAGACACAC CTTCCAGTAC CGCGTGTGGC ACTTTGTGGT GTCTCCGTCC 
TTTGAGTACA CCATTATGGC CATGATCGCC TTGAATACTG TTGTGCTGAT GATGAAGTAT 
TATTCTGCTC CCTGTACCTA TGAGCTGGCC CTGAAGTACC TGAATATCGC CTTCACCATG 
GTGTTTTCCC TGGAATGTGT CCTGAAGGTC ATCGCTTTTG GCTTTTTGAA CTATTTCCGA 
GACACCTGGA ATATCTTTGA CTTCATCACC GTGATTGGCA GTATCACAGA AATTATCCTG 
ACAGACAGCA AGCTGGTGAA CACCAGTGGC TTCAATATGA GCTTTCTGAA GCTCTTCCGA 
GCTGCCCGCC TCATAAAGCT CCTGCGTCAG GGCTATACCA TACGCATTTT GCTGTGGACC 
TTTGTGCAGT CCTTTAAGGC CCTCCCTTAT GTCTGCCTTT TAATTGCCAT GCTTTTCTTC 
ATTTATGCCA TCATTGGGAT GCAGGTATTT GGAAACATAA AATTAGACGA GGAGAGTCAC 
ATCAACCGGC ACAACAACTT CCGGAGTTTC TTTGGGTCCC TAATGCTACT CTTCAGGAGT 
GCCACAGGTG AGGCCTGGCA GGAGATTATG CTGTCATGCC TTGGGGAGAA GGGCTGTGAG 
CCTGACACCA CCGCACCATC AGGGCAGAAC GAGAACGAAC GCTGCGGCAC CGATCTGGCC 
TACGTGTACT TTGTCTCCTT CATCTTCTTC TGCTCCTTCT TGATGCTCAA CCTGTTTGTG 
GCCGTCATCA TGGACAACTT TGAGTACCTG ACTCGGGACT CCTCCATCCT GGGGCCTCAC 
CACTTGGACG AGTTTGTCCG CGTCTGGGCA GAATATGACC GAGCAGCATG CGGCAGGATC 
CCGTATAAGG ATATGTACAA ATTAGTGCGG GTGATCTCGC CTCCTCTGGG CCTGGGAGAG 
AACTGCCCCT ACAGGGTGGC TTGCAAGAGG TTGGTCCTGA TGAACATGCC AGTAGCTGAG 
GACATGACGG TCCACTTCAC CTCCACACTT ATGGCTCTGA TCCGGACAGC TCTGGACATT 
AAAATTGCCA AAGGTGGTGC AGACAGGCAG CAGCTAGACT CAGAGCTACA AAAGGAGACC 
CTAGCCATCT GGCCTCACCT ATCCCAGAAG ATGCTGGATC TGCTTGTGCC CATGCCCAAA 
GCCTCTGACC TGACTGTGGG CAAAATCTAT GCAGCAATGA TGATCATGGA CTACTATAAG 
CAGAGTAAGG TGAAGAAGCA GAGGCAGCAG CTGGAGGAAC AGAAAAATGC CCCCATGTTC 
CAGCGCATGG AGCCTTCATC TCTGCCTCAG GAGATCATTG CTAATGCCAA AGCCCTGCCT 
TACCTCCAGC AGGACCCCGT TTCAGGCCTG AGTGGCCGGA GTGGATACCC TTCGATGAGT 
CCACTCTCTC CCCAGGATAT ATTCCAGTTG GCTTGTATGG ACCCCGCCGA TGACGGACAG 
TTCCAAGAAC GGCAGTCTCT GGAGCCAGAG GTTAGTGAAT TAAAAAGCGT GCAGCCCTCT 
AACCATGGCA TCTACCTTCC TTCGGACACC CAGGAGCATG CGGGATCTGG GAGGGCATCT 
TCTATGCCAC GTCTGACTGT GGATCCCCAG GTGGTGACAG ACCCTAGCTC CATGAGACGT 
TCATTTTCCA CTATTCGGGA TAAGCGTTCA AATTCCTCGT GGTTGGAGGA ATTCTCCATG 
GAGCGAAGCA GTGAAAATAC CTACAAGTCC CGTCGCCGGA GTTACCACTC CTCCTTGCGG 
CTGTCAGCCC ACCGCCTGAA CTCTGATTCA GGCCACAAGT CTGACACTCA CCGCTCAGGG 
GGCAGGGAGC GGGGACGATC AAAAGAGCGA AAGCATCTTC TCTCTCCTGA TGTCTCCCGC 
TGCAATTCAG AAGAGCGAGG GACCCAGGCT GACTGGGAGT CCCCAGAGCG CCGTCAATCC 
AGGTCACCCA GTGAGGGCAG GTCACAGACG CCCAACAGAC AGGGCACAGG TTCCCTAAGT 
GAGAGCTCCA TCCCCTCTGT CTCTGACACC AGCACCCCAA GAAGAAGTCG TCGGCAGCTC 
CCACCCGTCC CGCCAAAGCC CCGGCCCCTC CTTTCCTACA GCTCCCTGAT TCGACACGCG 
GGCAGCATCT CTCCACCTGC TGATGGAAGC GAGGAGGGCT CCCCGCTGAC CTCCCAAGCT 
CTGGAGAGCA ACAATGCTTG CCTGACCGAG TCTTCCAACT CTCCGCACCC CCAGCAGAGC 
CAACATGCCT CCCCACAGCG CTACATCTCC GAGCCCTACT TGGCCCTGCA CGAAGACTCC 
CACGCCTCAG ACTGTGGTGA GGAGGAGACG CTCACTTTCG AAGCAGCCGT GGCTACTAGC 
CTGGGCCGTT CCAACACCAT CGGCTCAGCC CCACCCCTGC GGCATAGCTG GCAGATGCCC 
AACGGGCACT ATCGGCGGCG GAGGCGCGGG GGGCCTGGGC CAGGCATGAT GTGTGGGGCT 
GTCAACAACC TGCTAAGTGA CACGGAAGAA GATGACAAAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857735.1
CDS	427..7791
Translation	MVPEMRGLSPDRRLPEPAPCPAAGSPEPRSAGRRQRHVLALPEEEAWRPQQCALLEADASDEVGMLPASPRAASGFDNFFQVQEGEGQGWEGAMALEAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFEDRARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTSVTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALATNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIPYKDMYKLVRVISPPLGLGENCPYRVACKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLEPEVSELKSVQPSNHGIYLPSDTQEHAGSGRASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNNLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017002246.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002246.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC 
ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG 
TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG 
GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC 
TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC 
CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC 
CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC 
CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT 
CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG 
CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA 
GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA 
GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA 
GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG 
GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG 
GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG 
GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC 
ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC 
GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA 
GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG 
CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC 
TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT 
GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC 
GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC 
GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG 
TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG 
GCCTTTGCTC TGGCAACCAA CAAAGGACGG GACATCAAGA CCATCAAGTC TCTGCGGGTG 
CTCCGAGTTC TAAGGCCACT GAAAACCATC AAGCGCTTGC CCAAGCTCAA GGCCGTCTTC 
GACTGCGTAG TGACCTCCTT GAAGAATGTC TTCAACATAC TCATTGTGTA CAAGCTCTTC 
ATGTTCATCT TTGCTGTCAT CGCAGTTCAG CTCTTCAAGG GAAAGTTCTT TTATTGCACG 
GACAGTTCCA AGGACACAGA GAAGGAGTGC ATAGGCAACT ATGTAGATCA TGAGAAAAAC 
AAGATGGAGG TGAAGGGCCG GGAATGGAAG CGCCATGAAT TCCACTACGA CAACATTATC 
TGGGCCCTGC TGACCCTCTT CACCGTCTCC ACAGGGGAAG GATGGCCTCA AGTTCTGCAG 
CACTCTGTAG ATGTGACAGA GGAAGACCGA GGCCCAAGCC GCAGCAACCG CATGGAGATG 
TCTATCTTTT ATGTAGTCTA CTTTGTGGTC TTCCCCTTCT TCTTTGTCAA TATCTTTGTG 
GCTCTCATCA TCATCACCTT CCAGGAGCAA GGGGATAAGA TGATGGAGGA GTGCAGCCTG 
GAGAAGAATG AGAGGGCGTG CATCGACTTC GCCATCAGCG CCAAACCTCT CACCCGCTAC 
ATGCCGCAGA ACAGACACAC CTTCCAGTAC CGCGTGTGGC ACTTTGTGGT GTCTCCGTCC 
TTTGAGTACA CCATTATGGC CATGATCGCC TTGAATACTG TTGTGCTGAT GATGAAGTAT 
TATTCTGCTC CCTGTACCTA TGAGCTGGCC CTGAAGTACC TGAATATCGC CTTCACCATG 
GTGTTTTCCC TGGAATGTGT CCTGAAGGTC ATCGCTTTTG GCTTTTTGAA CTATTTCCGA 
GACACCTGGA ATATCTTTGA CTTCATCACC GTGATTGGCA GTATCACAGA AATTATCCTG 
ACAGACAGCA AGCTGGTGAA CACCAGTGGC TTCAATATGA GCTTTCTGAA GCTCTTCCGA 
GCTGCCCGCC TCATAAAGCT CCTGCGTCAG GGCTATACCA TACGCATTTT GCTGTGGACC 
TTTGTGCAGT CCTTTAAGGC CCTCCCTTAT GTCTGCCTTT TAATTGCCAT GCTTTTCTTC 
ATTTATGCCA TCATTGGGAT GCAGGTATTT GGAAACATAA AATTAGACGA GGAGAGTCAC 
ATCAACCGGC ACAACAACTT CCGGAGTTTC TTTGGGTCCC TAATGCTACT CTTCAGGAGT 
GCCACAGGTG AGGCCTGGCA GGAGATTATG CTGTCATGCC TTGGGGAGAA GGGCTGTGAG 
CCTGACACCA CCGCACCATC AGGGCAGAAC GAGAACGAAC GCTGCGGCAC CGATCTGGCC 
TACGTGTACT TTGTCTCCTT CATCTTCTTC TGCTCCTTCT TGATGCTCAA CCTGTTTGTG 
GCCGTCATCA TGGACAACTT TGAGTACCTG ACTCGGGACT CCTCCATCCT GGGGCCTCAC 
CACTTGGACG AGTTTGTCCG CGTCTGGGCA GAATATGACC GAGCAGCATG CGGCAGGATC 
CCGTATAAGG ATATGTACAA ATTAGTGCGG GTGATCTCGC CTCCTCTGGG CCTGGGAGAG 
AACTGCCCCT ACAGGGTGGC TTGCAAGAGG TTGGTCCTGA TGAACATGCC AGTAGCTGAG 
GACATGACGG TCCACTTCAC CTCCACACTT ATGGCTCTGA TCCGGACAGC TCTGGACATT 
AAAATTGCCA AAGGTGGTGC AGACAGGCAG CAGCTAGACT CAGAGCTACA AAAGGAGACC 
CTAGCCATCT GGCCTCACCT ATCCCAGAAG ATGCTGGATC TGCTTGTGCC CATGCCCAAA 
GCCTCTGACC TGACTGTGGG CAAAATCTAT GCAGCAATGA TGATCATGGA CTACTATAAG 
CAGAGTAAGG TGAAGAAGCA GAGGCAGCAG CTGGAGGAAC AGAAAAATGC CCCCATGTTC 
CAGCGCATGG AGCCTTCATC TCTGCCTCAG GAGATCATTG CTAATGCCAA AGCCCTGCCT 
TACCTCCAGC AGGACCCCGT TTCAGGCCTG AGTGGCCGGA GTGGATACCC TTCGATGAGT 
CCACTCTCTC CCCAGGATAT ATTCCAGTTG GCTTGTATGG ACCCCGCCGA TGACGGACAG 
TTCCAAGAAC GGCAGTCTCT GGAGCCAGAG GTTAGTGAAT TAAAAAGCGT GCAGCCCTCT 
AACCATGGCA TCTACCTTCC TTCGGACACC CAGGAGCATG CGGGATCTGG GAGGGCATCT 
TCTATGCCAC GTCTGACTGT GGATCCCCAG GTGGTGACAG ACCCTAGCTC CATGAGACGT 
TCATTTTCCA CTATTCGGGA TAAGCGTTCA AATTCCTCGT GGTTGGAGGA ATTCTCCATG 
GAGCGAAGCA GTGAAAATAC CTACAAGTCC CGTCGCCGGA GTTACCACTC CTCCTTGCGG 
CTGTCAGCCC ACCGCCTGAA CTCTGATTCA GGCCACAAGT CTGACACTCA CCGCTCAGGG 
GGCAGGGAGC GGGGACGATC AAAAGAGCGA AAGCATCTTC TCTCTCCTGA TGTCTCCCGC 
TGCAATTCAG AAGAGCGAGG GACCCAGGCT GACTGGGAGT CCCCAGAGCG CCGTCAATCC 
AGGTCACCCA GTGAGGGCAG GTCACAGACG CCCAACAGAC AGGGCACAGG TTCCCTAAGT 
GAGAGCTCCA TCCCCTCTGT CTCTGACACC AGCACCCCAA GAAGAAGTCG TCGGCAGCTC 
CCACCCGTCC CGCCAAAGCC CCGGCCCCTC CTTTCCTACA GCTCCCTGAT TCGACACGCG 
GGCAGCATCT CTCCACCTGC TGATGGAAGC GAGGAGGGCT CCCCGCTGAC CTCCCAAGCT 
CTGGAGAGCA ACAATGCTTG CCTGACCGAG TCTTCCAACT CTCCGCACCC CCAGCAGAGC 
CAACATGCCT CCCCACAGCG CTACATCTCC GAGCCCTACT TGGCCCTGCA CGAAGACTCC 
CACGCCTCAG ACTGTGGTGA GGAGGAGACG CTCACTTTCG AAGCAGCCGT GGCTACTAGC 
CTGGGCCGTT CCAACACCAT CGGCTCAGCC CCACCCCTGC GGCATAGCTG GCAGATGCCC 
AACGGGCACT ATCGGCGGCG GAGGCGCGGG GGGCCTGGGC CAGGCATGAT GTGTGGGGCT 
GTCAACAACC TGCTAAGTGA CACGGAAGAA GATGACAAAT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_017002247.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857736.1 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X5, mRNA.

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CloneID	OHu90477
Clone ID Related Accession (Same CDS sequence)	XM_017002247.1
Accession Version	XM_017002247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAGAGGG AGCGGAGGCG CCGGCACCAC 
ATGTCCGTGT GGGAGCAGCG TACCAGCCAG CTGAGGAAGC ACATGCAGAT GTCCAGCCAG 
GAGGCCCTCA ACAGAGAGGA GGCGCCGACC ATGAACCCGC TCAACCCCCT CAACCCGCTC 
AGCTCCCTCA ACCCGCTCAA TGCCCACCCC AGCCTTTATC GGCGACCCAG GGCCATTGAG 
GGCCTGGCCC TGGGCCTGGC CCTGGAGAAG TTCGAGGAGG AGCGCATCAG CCGTGGGGGG 
TCCCTCAAGG GGGATGGAGG GGACCGATCC AGTGCCCTGG ACAACCAGAG GACCCCTTTG 
TCCCTGGGCC AGCGGGAGCC ACCATGGCTG GCCAGGCCCT GTCATGGAAA CTGTGACCCG 
ACTCAGCAGG AGGCAGGGGG AGGAGAGGCT GTGGTGACCT TTGAGGACCG GGCCAGGCAC 
AGGCAGAGCC AACGGCGCAG CCGGCATCGC CGCGTCAGGA CAGAAGGCAA GGAGTCCTCT 
TCAGCCTCCC GGAGCAGGTC TGCCAGCCAG GAACGCAGTC TGGATGAAGC CATGCCCACT 
GAAGGGGAGA AGGACCATGA GCTCAGGGGC AACCATGGTG CCAAGGAGCC AACGATCCAA 
GAAGAGAGAG CCCAGGATTT AAGGAGGACC AACAGTCTGA TGGTGTCCAG AGGCTCCGGG 
CTGGCAGGAG GCCTTGATGA GGCTGACACC CCCCTAGTCC TGCCCCATCC TGAGCTGGAA 
GTGGGGAAGC ACGTGGTGCT GACGGAGCAG GAGCCAGAAG GCAGCAGTGA GCAGGCCCTG 
CTGGGGAATG TGCAGCTAGA CATGGGCCGG GTCATCAGCC AGAGCGAGCC TGACCTCTCC 
TGCATCACGG CCAACACGGA CAAGGCCACC ACCGAGAGCA CCAGCGTCAC CGTCGCCATC 
CCCGACGTGG ACCCCTTGGT GGACTCAACC GTGGTGCACA TTAGCAACAA GACGGATGGG 
GAAGCCAGTC CCTTGAAGGA GGCAGAGATC AGAGAGGATG AGGAGGAGGT GGAGAAGAAG 
AAGCAGAAGA AGGAGAAGCG TGAGACAGGC AAAGCCATGG TGCCCCACAG CTCAATGTTC 
ATCTTCAGCA CCACCAACCC GATCCGGAGG GCCTGCCACT ACATCGTGAA CCTGCGCTAC 
TTTGAGATGT GCATCCTCCT GGTGATTGCA GCCAGCAGCA TCGCCCTGGC GGCAGAGGAC 
CCCGTCCTGA CCAACTCGGA GCGCAACAAA GTCCTGAGGT ATTTTGACTA TGTGTTCACG 
GGCGTGTTCA CCTTTGAGAT GGTTATAAAG ATGATAGACC AAGGCTTGAT CCTGCAGGAT 
GGGTCCTACT TCCGAGACTT GTGGAACATC CTGGACTTTG TGGTGGTCGT TGGCGCATTG 
GTGGCCTTTG CTCTGGCGAA CGCTTTGGGA ACCAACAAAG GACGGGACAT CAAGACCATC 
AAGTCTCTGC GGGTGCTCCG AGTTCTAAGG CCACTGAAAA CCATCAAGCG CTTGCCCAAG 
CTCAAGGCCG TCTTCGACTG CGTAGTGACC TCCTTGAAGA ATGTCTTCAA CATACTCATT 
GTGTACAAGC TCTTCATGTT CATCTTTGCT GTCATCGCAG TTCAGCTCTT CAAGGGAAAG 
TTCTTTTATT GCACGGACAG TTCCAAGGAC ACAGAGAAGG AGTGCATAGG CAACTATGTA 
GATCATGAGA AAAACAAGAT GGAGGTGAAG GGCCGGGAAT GGAAGCGCCA TGAATTCCAC 
TACGACAACA TTATCTGGGC CCTGCTGACC CTCTTCACCG TCTCCACAGG GGAAGGATGG 
CCTCAAGTTC TGCAGCACTC TGTAGATGTG ACAGAGGAAG ACCGAGGCCC AAGCCGCAGC 
AACCGCATGG AGATGTCTAT CTTTTATGTA GTCTACTTTG TGGTCTTCCC CTTCTTCTTT 
GTCAATATCT TTGTGGCTCT CATCATCATC ACCTTCCAGG AGCAAGGGGA TAAGATGATG 
GAGGAGTGCA GCCTGGAGAA GAATGAGAGG GCGTGCATCG ACTTCGCCAT CAGCGCCAAA 
CCTCTCACCC GCTACATGCC GCAGAACAGA CACACCTTCC AGTACCGCGT GTGGCACTTT 
GTGGTGTCTC CGTCCTTTGA GTACACCATT ATGGCCATGA TCGCCTTGAA TACTGTTGTG 
CTGATGATGA AGTATTATTC TGCTCCCTGT ACCTATGAGC TGGCCCTGAA GTACCTGAAT 
ATCGCCTTCA CCATGGTGTT TTCCCTGGAA TGTGTCCTGA AGGTCATCGC TTTTGGCTTT 
TTGAACTATT TCCGAGACAC CTGGAATATC TTTGACTTCA TCACCGTGAT TGGCAGTATC 
ACAGAAATTA TCCTGACAGA CAGCAAGCTG GTGAACACCA GTGGCTTCAA TATGAGCTTT 
CTGAAGCTCT TCCGAGCTGC CCGCCTCATA AAGCTCCTGC GTCAGGGCTA TACCATACGC 
ATTTTGCTGT GGACCTTTGT GCAGTCCTTT AAGGCCCTCC CTTATGTCTG CCTTTTAATT 
GCCATGCTTT TCTTCATTTA TGCCATCATT GGGATGCAGG TATTTGGAAA CATAAAATTA 
GACGAGGAGA GTCACATCAA CCGGCACAAC AACTTCCGGA GTTTCTTTGG GTCCCTAATG 
CTACTCTTCA GGAGTGCCAC AGGTGAGGCC TGGCAGGAGA TTATGCTGTC ATGCCTTGGG 
GAGAAGGGCT GTGAGCCTGA CACCACCGCA CCATCAGGGC AGAACGAGAA CGAACGCTGC 
GGCACCGATC TGGCCTACGT GTACTTTGTC TCCTTCATCT TCTTCTGCTC CTTCTTGATG 
CTCAACCTGT TTGTGGCCGT CATCATGGAC AACTTTGAGT ACCTGACTCG GGACTCCTCC 
ATCCTGGGGC CTCACCACTT GGACGAGTTT GTCCGCGTCT GGGCAGAATA TGACCGAGCA 
GCATGTGGCC GCATCCATTA CACTGAGATG TATGAAATGC TGACTCTCAT GTCACCTCCG 
CTAGGCCTCG GCAAGAGATG TCCCTCCAAA GTGGCATATA AGAGGTTGGT CCTGATGAAC 
ATGCCAGTAG CTGAGGACAT GACGGTCCAC TTCACCTCCA CACTTATGGC TCTGATCCGG 
ACAGCTCTGG ACATTAAAAT TGCCAAAGGT GGTGCAGACA GGCAGCAGCT AGACTCAGAG 
CTACAAAAGG AGACCCTAGC CATCTGGCCT CACCTATCCC AGAAGATGCT GGATCTGCTT 
GTGCCCATGC CCAAAGCCTC TGACCTGACT GTGGGCAAAA TCTATGCAGC AATGATGATC 
ATGGACTACT ATAAGCAGAG TAAGGTGAAG AAGCAGAGGC AGCAGCTGGA GGAACAGAAA 
AATGCCCCCA TGTTCCAGCG CATGGAGCCT TCATCTCTGC CTCAGGAGAT CATTGCTAAT 
GCCAAAGCCC TGCCTTACCT CCAGCAGGAC CCCGTTTCAG GCCTGAGTGG CCGGAGTGGA 
TACCCTTCGA TGAGTCCACT CTCTCCCCAG GATATATTCC AGTTGGCTTG TATGGACCCC 
GCCGATGACG GACAGTTCCA AGAACGGCAG TCTCTGGAGC CAGAGGTTAG TGAATTAAAA 
AGCGTGCAGC CCTCTAACCA TGGCATCTAC CTTCCTTCGG ACACCCAGGA GCATGCGGGA 
TCTGGGAGGG CATCTTCTAT GCCACGTCTG ACTGTGGATC CCCAGGTGGT GACAGACCCT 
AGCTCCATGA GACGTTCATT TTCCACTATT CGGGATAAGC GTTCAAATTC CTCGTGGTTG 
GAGGAATTCT CCATGGAGCG AAGCAGTGAA AATACCTACA AGTCCCGTCG CCGGAGTTAC 
CACTCCTCCT TGCGGCTGTC AGCCCACCGC CTGAACTCTG ATTCAGGCCA CAAGTCTGAC 
ACTCACCGCT CAGGGGGCAG GGAGCGGGGA CGATCAAAAG AGCGAAAGCA TCTTCTCTCT 
CCTGATGTCT CCCGCTGCAA TTCAGAAGAG CGAGGGACCC AGGCTGACTG GGAGTCCCCA 
GAGCGCCGTC AATCCAGGTC ACCCAGTGAG GGCAGGTCAC AGACGCCCAA CAGACAGGGC 
ACAGGTTCCC TAAGTGAGAG CTCCATCCCC TCTGTCTCTG ACACCAGCAC CCCAAGAAGA 
AGTCGTCGGC AGCTCCCACC CGTCCCGCCA AAGCCCCGGC CCCTCCTTTC CTACAGCTCC 
CTGATTCGAC ACGCGGGCAG CATCTCTCCA CCTGCTGATG GAAGCGAGGA GGGCTCCCCG 
CTGACCTCCC AAGCTCTGGA GAGCAACAAT GCTTGCCTGA CCGAGTCTTC CAACTCTCCG 
CACCCCCAGC AGAGCCAACA TGCCTCCCCA CAGCGCTACA TCTCCGAGCC CTACTTGGCC 
CTGCACGAAG ACTCCCACGC CTCAGACTGT GGTGAGGAGG AGACGCTCAC TTTCGAAGCA 
GCCGTGGCTA CTAGCCTGGG CCGTTCCAAC ACCATCGGCT CAGCCCCACC CCTGCGGCAT 
AGCTGGCAGA TGCCCAACGG GCACTATCGG CGGCGGAGGC GCGGGGGGCC TGGGCCAGGC 
ATGATGTGTG GGGCTGTCAA CAACCTGCTA AGTGACACGG AAGAAGATGA CAAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857736.1
CDS	426..7745
Translation	MVPEMRGLSPDRRLPEPAPCPAAGSPEPRSAGRRQRHVLALPEEEAWRPQQCALLEADASDEVGMLPASPRAASGFDNFFQVQEGEGQGWEGAMALEAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFEDRARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTSVTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLEPEVSELKSVQPSNHGIYLPSDTQEHAGSGRASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNNLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017002247.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002247.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAGAGGG AGCGGAGGCG CCGGCACCAC 
ATGTCCGTGT GGGAGCAGCG TACCAGCCAG CTGAGGAAGC ACATGCAGAT GTCCAGCCAG 
GAGGCCCTCA ACAGAGAGGA GGCGCCGACC ATGAACCCGC TCAACCCCCT CAACCCGCTC 
AGCTCCCTCA ACCCGCTCAA TGCCCACCCC AGCCTTTATC GGCGACCCAG GGCCATTGAG 
GGCCTGGCCC TGGGCCTGGC CCTGGAGAAG TTCGAGGAGG AGCGCATCAG CCGTGGGGGG 
TCCCTCAAGG GGGATGGAGG GGACCGATCC AGTGCCCTGG ACAACCAGAG GACCCCTTTG 
TCCCTGGGCC AGCGGGAGCC ACCATGGCTG GCCAGGCCCT GTCATGGAAA CTGTGACCCG 
ACTCAGCAGG AGGCAGGGGG AGGAGAGGCT GTGGTGACCT TTGAGGACCG GGCCAGGCAC 
AGGCAGAGCC AACGGCGCAG CCGGCATCGC CGCGTCAGGA CAGAAGGCAA GGAGTCCTCT 
TCAGCCTCCC GGAGCAGGTC TGCCAGCCAG GAACGCAGTC TGGATGAAGC CATGCCCACT 
GAAGGGGAGA AGGACCATGA GCTCAGGGGC AACCATGGTG CCAAGGAGCC AACGATCCAA 
GAAGAGAGAG CCCAGGATTT AAGGAGGACC AACAGTCTGA TGGTGTCCAG AGGCTCCGGG 
CTGGCAGGAG GCCTTGATGA GGCTGACACC CCCCTAGTCC TGCCCCATCC TGAGCTGGAA 
GTGGGGAAGC ACGTGGTGCT GACGGAGCAG GAGCCAGAAG GCAGCAGTGA GCAGGCCCTG 
CTGGGGAATG TGCAGCTAGA CATGGGCCGG GTCATCAGCC AGAGCGAGCC TGACCTCTCC 
TGCATCACGG CCAACACGGA CAAGGCCACC ACCGAGAGCA CCAGCGTCAC CGTCGCCATC 
CCCGACGTGG ACCCCTTGGT GGACTCAACC GTGGTGCACA TTAGCAACAA GACGGATGGG 
GAAGCCAGTC CCTTGAAGGA GGCAGAGATC AGAGAGGATG AGGAGGAGGT GGAGAAGAAG 
AAGCAGAAGA AGGAGAAGCG TGAGACAGGC AAAGCCATGG TGCCCCACAG CTCAATGTTC 
ATCTTCAGCA CCACCAACCC GATCCGGAGG GCCTGCCACT ACATCGTGAA CCTGCGCTAC 
TTTGAGATGT GCATCCTCCT GGTGATTGCA GCCAGCAGCA TCGCCCTGGC GGCAGAGGAC 
CCCGTCCTGA CCAACTCGGA GCGCAACAAA GTCCTGAGGT ATTTTGACTA TGTGTTCACG 
GGCGTGTTCA CCTTTGAGAT GGTTATAAAG ATGATAGACC AAGGCTTGAT CCTGCAGGAT 
GGGTCCTACT TCCGAGACTT GTGGAACATC CTGGACTTTG TGGTGGTCGT TGGCGCATTG 
GTGGCCTTTG CTCTGGCGAA CGCTTTGGGA ACCAACAAAG GACGGGACAT CAAGACCATC 
AAGTCTCTGC GGGTGCTCCG AGTTCTAAGG CCACTGAAAA CCATCAAGCG CTTGCCCAAG 
CTCAAGGCCG TCTTCGACTG CGTAGTGACC TCCTTGAAGA ATGTCTTCAA CATACTCATT 
GTGTACAAGC TCTTCATGTT CATCTTTGCT GTCATCGCAG TTCAGCTCTT CAAGGGAAAG 
TTCTTTTATT GCACGGACAG TTCCAAGGAC ACAGAGAAGG AGTGCATAGG CAACTATGTA 
GATCATGAGA AAAACAAGAT GGAGGTGAAG GGCCGGGAAT GGAAGCGCCA TGAATTCCAC 
TACGACAACA TTATCTGGGC CCTGCTGACC CTCTTCACCG TCTCCACAGG GGAAGGATGG 
CCTCAAGTTC TGCAGCACTC TGTAGATGTG ACAGAGGAAG ACCGAGGCCC AAGCCGCAGC 
AACCGCATGG AGATGTCTAT CTTTTATGTA GTCTACTTTG TGGTCTTCCC CTTCTTCTTT 
GTCAATATCT TTGTGGCTCT CATCATCATC ACCTTCCAGG AGCAAGGGGA TAAGATGATG 
GAGGAGTGCA GCCTGGAGAA GAATGAGAGG GCGTGCATCG ACTTCGCCAT CAGCGCCAAA 
CCTCTCACCC GCTACATGCC GCAGAACAGA CACACCTTCC AGTACCGCGT GTGGCACTTT 
GTGGTGTCTC CGTCCTTTGA GTACACCATT ATGGCCATGA TCGCCTTGAA TACTGTTGTG 
CTGATGATGA AGTATTATTC TGCTCCCTGT ACCTATGAGC TGGCCCTGAA GTACCTGAAT 
ATCGCCTTCA CCATGGTGTT TTCCCTGGAA TGTGTCCTGA AGGTCATCGC TTTTGGCTTT 
TTGAACTATT TCCGAGACAC CTGGAATATC TTTGACTTCA TCACCGTGAT TGGCAGTATC 
ACAGAAATTA TCCTGACAGA CAGCAAGCTG GTGAACACCA GTGGCTTCAA TATGAGCTTT 
CTGAAGCTCT TCCGAGCTGC CCGCCTCATA AAGCTCCTGC GTCAGGGCTA TACCATACGC 
ATTTTGCTGT GGACCTTTGT GCAGTCCTTT AAGGCCCTCC CTTATGTCTG CCTTTTAATT 
GCCATGCTTT TCTTCATTTA TGCCATCATT GGGATGCAGG TATTTGGAAA CATAAAATTA 
GACGAGGAGA GTCACATCAA CCGGCACAAC AACTTCCGGA GTTTCTTTGG GTCCCTAATG 
CTACTCTTCA GGAGTGCCAC AGGTGAGGCC TGGCAGGAGA TTATGCTGTC ATGCCTTGGG 
GAGAAGGGCT GTGAGCCTGA CACCACCGCA CCATCAGGGC AGAACGAGAA CGAACGCTGC 
GGCACCGATC TGGCCTACGT GTACTTTGTC TCCTTCATCT TCTTCTGCTC CTTCTTGATG 
CTCAACCTGT TTGTGGCCGT CATCATGGAC AACTTTGAGT ACCTGACTCG GGACTCCTCC 
ATCCTGGGGC CTCACCACTT GGACGAGTTT GTCCGCGTCT GGGCAGAATA TGACCGAGCA 
GCATGTGGCC GCATCCATTA CACTGAGATG TATGAAATGC TGACTCTCAT GTCACCTCCG 
CTAGGCCTCG GCAAGAGATG TCCCTCCAAA GTGGCATATA AGAGGTTGGT CCTGATGAAC 
ATGCCAGTAG CTGAGGACAT GACGGTCCAC TTCACCTCCA CACTTATGGC TCTGATCCGG 
ACAGCTCTGG ACATTAAAAT TGCCAAAGGT GGTGCAGACA GGCAGCAGCT AGACTCAGAG 
CTACAAAAGG AGACCCTAGC CATCTGGCCT CACCTATCCC AGAAGATGCT GGATCTGCTT 
GTGCCCATGC CCAAAGCCTC TGACCTGACT GTGGGCAAAA TCTATGCAGC AATGATGATC 
ATGGACTACT ATAAGCAGAG TAAGGTGAAG AAGCAGAGGC AGCAGCTGGA GGAACAGAAA 
AATGCCCCCA TGTTCCAGCG CATGGAGCCT TCATCTCTGC CTCAGGAGAT CATTGCTAAT 
GCCAAAGCCC TGCCTTACCT CCAGCAGGAC CCCGTTTCAG GCCTGAGTGG CCGGAGTGGA 
TACCCTTCGA TGAGTCCACT CTCTCCCCAG GATATATTCC AGTTGGCTTG TATGGACCCC 
GCCGATGACG GACAGTTCCA AGAACGGCAG TCTCTGGAGC CAGAGGTTAG TGAATTAAAA 
AGCGTGCAGC CCTCTAACCA TGGCATCTAC CTTCCTTCGG ACACCCAGGA GCATGCGGGA 
TCTGGGAGGG CATCTTCTAT GCCACGTCTG ACTGTGGATC CCCAGGTGGT GACAGACCCT 
AGCTCCATGA GACGTTCATT TTCCACTATT CGGGATAAGC GTTCAAATTC CTCGTGGTTG 
GAGGAATTCT CCATGGAGCG AAGCAGTGAA AATACCTACA AGTCCCGTCG CCGGAGTTAC 
CACTCCTCCT TGCGGCTGTC AGCCCACCGC CTGAACTCTG ATTCAGGCCA CAAGTCTGAC 
ACTCACCGCT CAGGGGGCAG GGAGCGGGGA CGATCAAAAG AGCGAAAGCA TCTTCTCTCT 
CCTGATGTCT CCCGCTGCAA TTCAGAAGAG CGAGGGACCC AGGCTGACTG GGAGTCCCCA 
GAGCGCCGTC AATCCAGGTC ACCCAGTGAG GGCAGGTCAC AGACGCCCAA CAGACAGGGC 
ACAGGTTCCC TAAGTGAGAG CTCCATCCCC TCTGTCTCTG ACACCAGCAC CCCAAGAAGA 
AGTCGTCGGC AGCTCCCACC CGTCCCGCCA AAGCCCCGGC CCCTCCTTTC CTACAGCTCC 
CTGATTCGAC ACGCGGGCAG CATCTCTCCA CCTGCTGATG GAAGCGAGGA GGGCTCCCCG 
CTGACCTCCC AAGCTCTGGA GAGCAACAAT GCTTGCCTGA CCGAGTCTTC CAACTCTCCG 
CACCCCCAGC AGAGCCAACA TGCCTCCCCA CAGCGCTACA TCTCCGAGCC CTACTTGGCC 
CTGCACGAAG ACTCCCACGC CTCAGACTGT GGTGAGGAGG AGACGCTCAC TTTCGAAGCA 
GCCGTGGCTA CTAGCCTGGG CCGTTCCAAC ACCATCGGCT CAGCCCCACC CCTGCGGCAT 
AGCTGGCAGA TGCCCAACGG GCACTATCGG CGGCGGAGGC GCGGGGGGCC TGGGCCAGGC 
ATGATGTGTG GGGCTGTCAA CAACCTGCTA AGTGACACGG AAGAAGATGA CAAATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_017002248.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857737.1 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X6, mRNA.

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CloneID	OHu90478
Clone ID Related Accession (Same CDS sequence)	XM_017002248.1
Accession Version	XM_017002248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC 
ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG 
TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG 
GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC 
TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC 
CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC 
CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC 
CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT 
CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG 
CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA 
GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA 
GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA 
GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG 
GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG 
GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG 
GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC 
ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC 
GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA 
GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG 
CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC 
TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT 
GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC 
GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC 
GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG 
TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG 
GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG 
TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC 
AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG 
TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC 
TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT 
CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC 
GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT 
CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC 
CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC 
AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG 
GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT 
CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG 
GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG 
ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC 
GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG 
AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA 
GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG 
AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT 
TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GTATTTGGAA ACATAAAATT AGACGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTAAT GCTACTCTTC 
AGGAGTGCCA CAGGTGAGGC CTGGCAGGAG ATTATGCTGT CATGCCTTGG GGAGAAGGGC 
TGTGAGCCTG ACACCACCGC ACCATCAGGG CAGAACGAGA ACGAACGCTG CGGCACCGAT 
CTGGCCTACG TGTACTTTGT CTCCTTCATC TTCTTCTGCT CCTTCTTGAT GCTCAACCTG 
TTTGTGGCCG TCATCATGGA CAACTTTGAG TACCTGACTC GGGACTCCTC CATCCTGGGG 
CCTCACCACT TGGACGAGTT TGTCCGCGTC TGGGCAGAAT ATGACCGAGC AGCATGTGGC 
CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCTCC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA AGTGGCATAT AAGAGGTTGG TCCTGATGAA CATGCCAGTA 
GCTGAGGACA TGACGGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG 
GACATTAAAA TTGCCAAAGG TGGTGCAGAC AGGCAGCAGC TAGACTCAGA GCTACAAAAG 
GAGACCCTAG CCATCTGGCC TCACCTATCC CAGAAGATGC TGGATCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAGCAGA GTAAGGTGAA GAAGCAGAGG CAGCAGCTGG AGGAACAGAA AAATGCCCCC 
ATGTTCCAGC GCATGGAGCC TTCATCTCTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCTTACC TCCAGCAGGA CCCCGTTTCA GGCCTGAGTG GCCGGAGTGG ATACCCTTCG 
ATGAGTCCAC TCTCTCCCCA GGATATATTC CAGTTGGCTT GTATGGACCC CGCCGATGAC 
GGACAGTTCC AAGAACGGCA GTCTCTGGAG CCAGAGGTTA GTGAATTAAA AAGCGTGCAG 
CCCTCTAACC ATGGCATCTA CCTTCCTTCG GACACCCAGG AGCATGCGGG ATCTGGGAGG 
GCATCTTCTA TGCCACGTCT GACTGTGGAT CCCCAGGTGG TGACAGACCC TAGCTCCATG 
AGACGTTCAT TTTCCACTAT TCGGGATAAG CGTTCAAATT CCTCGTGGTT GGAGGAATTC 
TCCATGGAGC GAAGCAGTGA AAATACCTAC AAGTCCCGTC GCCGGAGTTA CCACTCCTCC 
TTGCGGCTGT CAGCCCACCG CCTGAACTCT GATTCAGGCC ACAAGTCTGA CACTCACCGC 
TCAGGGGGCA GGGAGCGGGG ACGATCAAAA GAGCGAAAGC ATCTTCTCTC TCCTGATGTC 
TCCCGCTGCA ATTCAGAAGA GCGAGGGACC CAGGCTGACT GGGAGTCCCC AGAGCGCCGT 
CAATCCAGGT CACCCAGTGA GGGCAGGTCA CAGACGCCCA ACAGACAGGG CACAGGTTCC 
CTAAGTGAGA GCTCCATCCC CTCTGTCTCT GACACCAGCA CCCCAAGAAG AAGTCGTCGG 
CAGCTCCCAC CCGTCCCGCC AAAGCCCCGG CCCCTCCTTT CCTACAGCTC CCTGATTCGA 
CACGCGGGCA GCATCTCTCC ACCTGCTGAT GGAAGCGAGG AGGGCTCCCC GCTGACCTCC 
CAAGCTCTGG AGAGCAACAA TGCTTGCCTG ACCGAGTCTT CCAACTCTCC GCACCCCCAG 
CAGAGCCAAC ATGCCTCCCC ACAGCGCTAC ATCTCCGAGC CCTACTTGGC CCTGCACGAA 
GACTCCCACG CCTCAGACTG TGGTGAGGAG GAGACGCTCA CTTTCGAAGC AGCCGTGGCT 
ACTAGCCTGG GCCGTTCCAA CACCATCGGC TCAGCCCCAC CCCTGCGGCA TAGCTGGCAG 
ATGCCCAACG GGCACTATCG GCGGCGGAGG CGCGGGGGGC CTGGGCCAGG CATGATGTGT 
GGGGCTGTCA ACAACCTGCT AAGTGACACG GAAGAAGATG ACAAATGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857737.1
CDS	427..7737
Translation	MVPEMRGLSPDRRLPEPAPCPAAGSPEPRSAGRRQRHVLALPEEEAWRPQQCALLEADASDEVGMLPASPRAASGFDNFFQVQEGEGQGWEGAMALEAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFEDRARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTSVTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLEPEVSELKSVQPSNHGIYLPSDTQEHAGSGRASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNNLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017002248.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002248.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC 
ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG 
TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG 
GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC 
TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC 
CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC 
CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC 
CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT 
CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG 
CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA 
GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA 
GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA 
GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG 
GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG 
GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG 
GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC 
ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC 
GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA 
GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG 
CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC 
TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT 
GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC 
GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC 
GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG 
TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG 
GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG 
TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC 
AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG 
TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC 
TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT 
CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC 
GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT 
CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC 
CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC 
AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG 
GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT 
CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG 
GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG 
ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC 
GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG 
AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA 
GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG 
AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT 
TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GTATTTGGAA ACATAAAATT AGACGAGGAG 
AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTAAT GCTACTCTTC 
AGGAGTGCCA CAGGTGAGGC CTGGCAGGAG ATTATGCTGT CATGCCTTGG GGAGAAGGGC 
TGTGAGCCTG ACACCACCGC ACCATCAGGG CAGAACGAGA ACGAACGCTG CGGCACCGAT 
CTGGCCTACG TGTACTTTGT CTCCTTCATC TTCTTCTGCT CCTTCTTGAT GCTCAACCTG 
TTTGTGGCCG TCATCATGGA CAACTTTGAG TACCTGACTC GGGACTCCTC CATCCTGGGG 
CCTCACCACT TGGACGAGTT TGTCCGCGTC TGGGCAGAAT ATGACCGAGC AGCATGTGGC 
CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCTCC GCTAGGCCTC 
GGCAAGAGAT GTCCCTCCAA AGTGGCATAT AAGAGGTTGG TCCTGATGAA CATGCCAGTA 
GCTGAGGACA TGACGGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG 
GACATTAAAA TTGCCAAAGG TGGTGCAGAC AGGCAGCAGC TAGACTCAGA GCTACAAAAG 
GAGACCCTAG CCATCTGGCC TCACCTATCC CAGAAGATGC TGGATCTGCT TGTGCCCATG 
CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC 
TATAAGCAGA GTAAGGTGAA GAAGCAGAGG CAGCAGCTGG AGGAACAGAA AAATGCCCCC 
ATGTTCCAGC GCATGGAGCC TTCATCTCTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC 
CTGCCTTACC TCCAGCAGGA CCCCGTTTCA GGCCTGAGTG GCCGGAGTGG ATACCCTTCG 
ATGAGTCCAC TCTCTCCCCA GGATATATTC CAGTTGGCTT GTATGGACCC CGCCGATGAC 
GGACAGTTCC AAGAACGGCA GTCTCTGGAG CCAGAGGTTA GTGAATTAAA AAGCGTGCAG 
CCCTCTAACC ATGGCATCTA CCTTCCTTCG GACACCCAGG AGCATGCGGG ATCTGGGAGG 
GCATCTTCTA TGCCACGTCT GACTGTGGAT CCCCAGGTGG TGACAGACCC TAGCTCCATG 
AGACGTTCAT TTTCCACTAT TCGGGATAAG CGTTCAAATT CCTCGTGGTT GGAGGAATTC 
TCCATGGAGC GAAGCAGTGA AAATACCTAC AAGTCCCGTC GCCGGAGTTA CCACTCCTCC 
TTGCGGCTGT CAGCCCACCG CCTGAACTCT GATTCAGGCC ACAAGTCTGA CACTCACCGC 
TCAGGGGGCA GGGAGCGGGG ACGATCAAAA GAGCGAAAGC ATCTTCTCTC TCCTGATGTC 
TCCCGCTGCA ATTCAGAAGA GCGAGGGACC CAGGCTGACT GGGAGTCCCC AGAGCGCCGT 
CAATCCAGGT CACCCAGTGA GGGCAGGTCA CAGACGCCCA ACAGACAGGG CACAGGTTCC 
CTAAGTGAGA GCTCCATCCC CTCTGTCTCT GACACCAGCA CCCCAAGAAG AAGTCGTCGG 
CAGCTCCCAC CCGTCCCGCC AAAGCCCCGG CCCCTCCTTT CCTACAGCTC CCTGATTCGA 
CACGCGGGCA GCATCTCTCC ACCTGCTGAT GGAAGCGAGG AGGGCTCCCC GCTGACCTCC 
CAAGCTCTGG AGAGCAACAA TGCTTGCCTG ACCGAGTCTT CCAACTCTCC GCACCCCCAG 
CAGAGCCAAC ATGCCTCCCC ACAGCGCTAC ATCTCCGAGC CCTACTTGGC CCTGCACGAA 
GACTCCCACG CCTCAGACTG TGGTGAGGAG GAGACGCTCA CTTTCGAAGC AGCCGTGGCT 
ACTAGCCTGG GCCGTTCCAA CACCATCGGC TCAGCCCCAC CCCTGCGGCA TAGCTGGCAG 
ATGCCCAACG GGCACTATCG GCGGCGGAGG CGCGGGGGGC CTGGGCCAGG CATGATGTGT 
GGGGCTGTCA ACAACCTGCT AAGTGACACG GAAGAAGATG ACAAATGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_017002249.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857738.1 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X7, mRNA.

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CloneID	OHu90479
Clone ID Related Accession (Same CDS sequence)	XM_017002249.1
Accession Version	XM_017002249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC 
ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG 
TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG 
GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC 
TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC 
CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC 
CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC 
CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT 
CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG 
CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA 
GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA 
GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA 
GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG 
GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG 
GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG 
GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC 
ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC 
GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA 
GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG 
CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC 
TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT 
GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC 
GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC 
GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG 
TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG 
GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG 
TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC 
AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG 
TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC 
TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT 
CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC 
GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT 
CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC 
CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC 
AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG 
GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT 
CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG 
GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG 
ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC 
GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG 
AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA 
GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG 
AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT 
TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GCCCTCCCTT ATGTCTGCCT TTTAATTGCC 
ATGCTTTTCT TCATTTATGC CATCATTGGG ATGCAGGTAT TTGGAAACAT AAAATTAGAC 
GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGGTC CCTAATGCTA 
CTCTTCAGGA GTGCCACAGG TGAGGCCTGG CAGGAGATTA TGCTGTCATG CCTTGGGGAG 
AAGGGCTGTG AGCCTGACAC CACCGCACCA TCAGGGCAGA ACGAGAACGA ACGCTGCGGC 
ACCGATCTGG CCTACGTGTA CTTTGTCTCC TTCATCTTCT TCTGCTCCTT CTTGATGCTC 
AACCTGTTTG TGGCCGTCAT CATGGACAAC TTTGAGTACC TGACTCGGGA CTCCTCCATC 
CTGGGGCCTC ACCACTTGGA CGAGTTTGTC CGCGTCTGGG CAGAATATGA CCGAGCAGCA 
TGTGGCCGCA TCCATTACAC TGAGATGTAT GAAATGCTGA CTCTCATGTC ACCTCCGCTA 
GGCCTCGGCA AGAGATGTCC CTCCAAAGTG GCATATAAGA GGTTGGTCCT GATGAACATG 
CCAGTAGCTG AGGACATGAC GGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA 
GCTCTGGACA TTAAAATTGC CAAAGGTGGT GCAGACAGGC AGCAGCTAGA CTCAGAGCTA 
CAAAAGGAGA CCCTAGCCAT CTGGCCTCAC CTATCCCAGA AGATGCTGGA TCTGCTTGTG 
CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAAATCT ATGCAGCAAT GATGATCATG 
GACTACTATA AGCAGAGTAA GGTGAAGAAG CAGAGGCAGC AGCTGGAGGA ACAGAAAAAT 
GCCCCCATGT TCCAGCGCAT GGAGCCTTCA TCTCTGCCTC AGGAGATCAT TGCTAATGCC 
AAAGCCCTGC CTTACCTCCA GCAGGACCCC GTTTCAGGCC TGAGTGGCCG GAGTGGATAC 
CCTTCGATGA GTCCACTCTC TCCCCAGGAT ATATTCCAGT TGGCTTGTAT GGACCCCGCC 
GATGACGGAC AGTTCCAAGA ACGGCAGTCT CTGGTGGTGA CAGACCCTAG CTCCATGAGA 
CGTTCATTTT CCACTATTCG GGATAAGCGT TCAAATTCCT CGTGGTTGGA GGAATTCTCC 
ATGGAGCGAA GCAGTGAAAA TACCTACAAG TCCCGTCGCC GGAGTTACCA CTCCTCCTTG 
CGGCTGTCAG CCCACCGCCT GAACTCTGAT TCAGGCCACA AGTCTGACAC TCACCGCTCA 
GGGGGCAGGG AGCGGGGACG ATCAAAAGAG CGAAAGCATC TTCTCTCTCC TGATGTCTCC 
CGCTGCAATT CAGAAGAGCG AGGGACCCAG GCTGACTGGG AGTCCCCAGA GCGCCGTCAA 
TCCAGGTCAC CCAGTGAGGG CAGGTCACAG ACGCCCAACA GACAGGGCAC AGGTTCCCTA 
AGTGAGAGCT CCATCCCCTC TGTCTCTGAC ACCAGCACCC CAAGAAGAAG TCGTCGGCAG 
CTCCCACCCG TCCCGCCAAA GCCCCGGCCC CTCCTTTCCT ACAGCTCCCT GATTCGACAC 
GCGGGCAGCA TCTCTCCACC TGCTGATGGA AGCGAGGAGG GCTCCCCGCT GACCTCCCAA 
GCTCTGGAGA GCAACAATGC TTGCCTGACC GAGTCTTCCA ACTCTCCGCA CCCCCAGCAG 
AGCCAACATG CCTCCCCACA GCGCTACATC TCCGAGCCCT ACTTGGCCCT GCACGAAGAC 
TCCCACGCCT CAGACTGTGG TGAGGAGGAG ACGCTCACTT TCGAAGCAGC CGTGGCTACT 
AGCCTGGGCC GTTCCAACAC CATCGGCTCA GCCCCACCCC TGCGGCATAG CTGGCAGATG 
CCCAACGGGC ACTATCGGCG GCGGAGGCGC GGGGGGCCTG GGCCAGGCAT GATGTGTGGG 
GCTGTCAACA ACCTGCTAAG TGACACGGAA GAAGATGACA AATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857738.1
CDS	426..7673
Translation	MVPEMRGLSPDRRLPEPAPCPAAGSPEPRSAGRRQRHVLALPEEEAWRPQQCALLEADASDEVGMLPASPRAASGFDNFFQVQEGEGQGWEGAMALEAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFEDRARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTSVTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNNLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017002249.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002249.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC 
ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG 
TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG 
GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC 
TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC 
CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC 
CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC 
CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT 
CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG 
CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA 
GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA 
GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA 
GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG 
GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG 
GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG 
GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC 
ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC 
GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA 
GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG 
CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC 
TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT 
GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC 
GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC 
GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG 
TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG 
GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG 
TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC 
AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG 
TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC 
TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT 
CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC 
GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT 
CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC 
CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC 
AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG 
GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT 
CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG 
GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG 
ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC 
GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG 
AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA 
GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG 
AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT 
TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GCCCTCCCTT ATGTCTGCCT TTTAATTGCC 
ATGCTTTTCT TCATTTATGC CATCATTGGG ATGCAGGTAT TTGGAAACAT AAAATTAGAC 
GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGGTC CCTAATGCTA 
CTCTTCAGGA GTGCCACAGG TGAGGCCTGG CAGGAGATTA TGCTGTCATG CCTTGGGGAG 
AAGGGCTGTG AGCCTGACAC CACCGCACCA TCAGGGCAGA ACGAGAACGA ACGCTGCGGC 
ACCGATCTGG CCTACGTGTA CTTTGTCTCC TTCATCTTCT TCTGCTCCTT CTTGATGCTC 
AACCTGTTTG TGGCCGTCAT CATGGACAAC TTTGAGTACC TGACTCGGGA CTCCTCCATC 
CTGGGGCCTC ACCACTTGGA CGAGTTTGTC CGCGTCTGGG CAGAATATGA CCGAGCAGCA 
TGTGGCCGCA TCCATTACAC TGAGATGTAT GAAATGCTGA CTCTCATGTC ACCTCCGCTA 
GGCCTCGGCA AGAGATGTCC CTCCAAAGTG GCATATAAGA GGTTGGTCCT GATGAACATG 
CCAGTAGCTG AGGACATGAC GGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA 
GCTCTGGACA TTAAAATTGC CAAAGGTGGT GCAGACAGGC AGCAGCTAGA CTCAGAGCTA 
CAAAAGGAGA CCCTAGCCAT CTGGCCTCAC CTATCCCAGA AGATGCTGGA TCTGCTTGTG 
CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAAATCT ATGCAGCAAT GATGATCATG 
GACTACTATA AGCAGAGTAA GGTGAAGAAG CAGAGGCAGC AGCTGGAGGA ACAGAAAAAT 
GCCCCCATGT TCCAGCGCAT GGAGCCTTCA TCTCTGCCTC AGGAGATCAT TGCTAATGCC 
AAAGCCCTGC CTTACCTCCA GCAGGACCCC GTTTCAGGCC TGAGTGGCCG GAGTGGATAC 
CCTTCGATGA GTCCACTCTC TCCCCAGGAT ATATTCCAGT TGGCTTGTAT GGACCCCGCC 
GATGACGGAC AGTTCCAAGA ACGGCAGTCT CTGGTGGTGA CAGACCCTAG CTCCATGAGA 
CGTTCATTTT CCACTATTCG GGATAAGCGT TCAAATTCCT CGTGGTTGGA GGAATTCTCC 
ATGGAGCGAA GCAGTGAAAA TACCTACAAG TCCCGTCGCC GGAGTTACCA CTCCTCCTTG 
CGGCTGTCAG CCCACCGCCT GAACTCTGAT TCAGGCCACA AGTCTGACAC TCACCGCTCA 
GGGGGCAGGG AGCGGGGACG ATCAAAAGAG CGAAAGCATC TTCTCTCTCC TGATGTCTCC 
CGCTGCAATT CAGAAGAGCG AGGGACCCAG GCTGACTGGG AGTCCCCAGA GCGCCGTCAA 
TCCAGGTCAC CCAGTGAGGG CAGGTCACAG ACGCCCAACA GACAGGGCAC AGGTTCCCTA 
AGTGAGAGCT CCATCCCCTC TGTCTCTGAC ACCAGCACCC CAAGAAGAAG TCGTCGGCAG 
CTCCCACCCG TCCCGCCAAA GCCCCGGCCC CTCCTTTCCT ACAGCTCCCT GATTCGACAC 
GCGGGCAGCA TCTCTCCACC TGCTGATGGA AGCGAGGAGG GCTCCCCGCT GACCTCCCAA 
GCTCTGGAGA GCAACAATGC TTGCCTGACC GAGTCTTCCA ACTCTCCGCA CCCCCAGCAG 
AGCCAACATG CCTCCCCACA GCGCTACATC TCCGAGCCCT ACTTGGCCCT GCACGAAGAC 
TCCCACGCCT CAGACTGTGG TGAGGAGGAG ACGCTCACTT TCGAAGCAGC CGTGGCTACT 
AGCCTGGGCC GTTCCAACAC CATCGGCTCA GCCCCACCCC TGCGGCATAG CTGGCAGATG 
CCCAACGGGC ACTATCGGCG GCGGAGGCGC GGGGGGCCTG GGCCAGGCAT GATGTGTGGG 
GCTGTCAACA ACCTGCTAAG TGACACGGAA GAAGATGACA AATGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1E ( XM_017002250.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016857739.1 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X8, mRNA.

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CloneID	OHu90480
Clone ID Related Accession (Same CDS sequence)	XM_017002250.1
Accession Version	XM_017002250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent R-type calcium channel subunit alpha-1E isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAGAGGG AGCGGAGGCG CCGGCACCAC 
ATGTCCGTGT GGGAGCAGCG TACCAGCCAG CTGAGGAAGC ACATGCAGAT GTCCAGCCAG 
GAGGCCCTCA ACAGAGAGGA GGCGCCGACC ATGAACCCGC TCAACCCCCT CAACCCGCTC 
AGCTCCCTCA ACCCGCTCAA TGCCCACCCC AGCCTTTATC GGCGACCCAG GGCCATTGAG 
GGCCTGGCCC TGGGCCTGGC CCTGGAGAAG TTCGAGGAGG AGCGCATCAG CCGTGGGGGG 
TCCCTCAAGG GGGATGGAGG GGACCGATCC AGTGCCCTGG ACAACCAGAG GACCCCTTTG 
TCCCTGGGCC AGCGGGAGCC ACCATGGCTG GCCAGGCCCT GTCATGGAAA CTGTGACCCG 
ACTCAGCAGG AGGCAGGGGG AGGAGAGGCT GTGGTGACCT TTGAGGACCG GGCCAGGCAC 
AGGCAGAGCC AACGGCGCAG CCGGCATCGC CGCGTCAGGA CAGAAGGCAA GGAGTCCTCT 
TCAGCCTCCC GGAGCAGGTC TGCCAGCCAG GAACGCAGTC TGGATGAAGC CATGCCCACT 
GAAGGGGAGA AGGACCATGA GCTCAGGGGC AACCATGGTG CCAAGGAGCC AACGATCCAA 
GAAGAGAGAG CCCAGGATTT AAGGAGGACC AACAGTCTGA TGGTGTCCAG AGGCTCCGGG 
CTGGCAGGAG GCCTTGATGA GGCTGACACC CCCCTAGTCC TGCCCCATCC TGAGCTGGAA 
GTGGGGAAGC ACGTGGTGCT GACGGAGCAG GAGCCAGAAG GCAGCAGTGA GCAGGCCCTG 
CTGGGGAATG TGCAGCTAGA CATGGGCCGG GTCATCAGCC AGAGCGAGCC TGACCTCTCC 
TGCATCACGG CCAACACGGA CAAGGCCACC ACCGAGAGCA CCAGCGTCAC CGTCGCCATC 
CCCGACGTGG ACCCCTTGGT GGACTCAACC GTGGTGCACA TTAGCAACAA GACGGATGGG 
GAAGCCAGTC CCTTGAAGGA GGCAGAGATC AGAGAGGATG AGGAGGAGGT GGAGAAGAAG 
AAGCAGAAGA AGGAGAAGCG TGAGACAGGC AAAGCCATGG TGCCCCACAG CTCAATGTTC 
ATCTTCAGCA CCACCAACCC GATCCGGAGG GCCTGCCACT ACATCGTGAA CCTGCGCTAC 
TTTGAGATGT GCATCCTCCT GGTGATTGCA GCCAGCAGCA TCGCCCTGGC GGCAGAGGAC 
CCCGTCCTGA CCAACTCGGA GCGCAACAAA GTCCTGAGGT ATTTTGACTA TGTGTTCACG 
GGCGTGTTCA CCTTTGAGAT GGTTATAAAG ATGATAGACC AAGGCTTGAT CCTGCAGGAT 
GGGTCCTACT TCCGAGACTT GTGGAACATC CTGGACTTTG TGGTGGTCGT TGGCGCATTG 
GTGGCCTTTG CTCTGGCGAA CGCTTTGGGA ACCAACAAAG GACGGGACAT CAAGACCATC 
AAGTCTCTGC GGGTGCTCCG AGTTCTAAGG CCACTGAAAA CCATCAAGCG CTTGCCCAAG 
CTCAAGGCCG TCTTCGACTG CGTAGTGACC TCCTTGAAGA ATGTCTTCAA CATACTCATT 
GTGTACAAGC TCTTCATGTT CATCTTTGCT GTCATCGCAG TTCAGCTCTT CAAGGGAAAG 
TTCTTTTATT GCACGGACAG TTCCAAGGAC ACAGAGAAGG AGTGCATAGG CAACTATGTA 
GATCATGAGA AAAACAAGAT GGAGGTGAAG GGCCGGGAAT GGAAGCGCCA TGAATTCCAC 
TACGACAACA TTATCTGGGC CCTGCTGACC CTCTTCACCG TCTCCACAGG GGAAGGATGG 
CCTCAAGTTC TGCAGCACTC TGTAGATGTG ACAGAGGAAG ACCGAGGCCC AAGCCGCAGC 
AACCGCATGG AGATGTCTAT CTTTTATGTA GTCTACTTTG TGGTCTTCCC CTTCTTCTTT 
GTCAATATCT TTGTGGCTCT CATCATCATC ACCTTCCAGG AGCAAGGGGA TAAGATGATG 
GAGGAGTGCA GCCTGGAGAA GAATGAGAGG GCGTGCATCG ACTTCGCCAT CAGCGCCAAA 
CCTCTCACCC GCTACATGCC GCAGAACAGA CACACCTTCC AGTACCGCGT GTGGCACTTT 
GTGGTGTCTC CGTCCTTTGA GTACACCATT ATGGCCATGA TCGCCTTGAA TACTGTTGTG 
CTGATGATGA AGTATTATTC TGCTCCCTGT ACCTATGAGC TGGCCCTGAA GTACCTGAAT 
ATCGCCTTCA CCATGGTGTT TTCCCTGGAA TGTGTCCTGA AGGTCATCGC TTTTGGCTTT 
TTGAACTATT TCCGAGACAC CTGGAATATC TTTGACTTCA TCACCGTGAT TGGCAGTATC 
ACAGAAATTA TCCTGACAGA CAGCAAGCTG GTGAACACCA GTGGCTTCAA TATGAGCTTT 
CTGAAGCTCT TCCGAGCTGC CCGCCTCATA AAGCTCCTGC GTCAGGGCTA TACCATACGC 
ATTTTGCTGT GGACCTTTGT GCAGTCCTTT AAGGCCCTCC CTTATGTCTG CCTTTTAATT 
GCCATGCTTT TCTTCATTTA TGCCATCATT GGGATGCAGG TATTTGGAAA CATAAAATTA 
GACGAGGAGA GTCACATCAA CCGGCACAAC AACTTCCGGA GTTTCTTTGG GTCCCTAATG 
CTACTCTTCA GGAGTGCCAC AGGTGAGGCC TGGCAGGAGA TTATGCTGTC ATGCCTTGGG 
GAGAAGGGCT GTGAGCCTGA CACCACCGCA CCATCAGGGC AGAACGAGAA CGAACGCTGC 
GGCACCGATC TGGCCTACGT GTACTTTGTC TCCTTCATCT TCTTCTGCTC CTTCTTGATG 
CTCAACCTGT TTGTGGCCGT CATCATGGAC AACTTTGAGT ACCTGACTCG GGACTCCTCC 
ATCCTGGGGC CTCACCACTT GGACGAGTTT GTCCGCGTCT GGGCAGAATA TGACCGAGCA 
GCATGTGGCC GCATCCATTA CACTGAGATG TATGAAATGC TGACTCTCAT GTCACCTCCG 
CTAGGCCTCG GCAAGAGATG TCCCTCCAAA GTGGCATATA AGAGGTTGGT CCTGATGAAC 
ATGCCAGTAG CTGAGGACAT GACGGTCCAC TTCACCTCCA CACTTATGGC TCTGATCCGG 
ACAGCTCTGG ACATTAAAAT TGCCAAAGGT GGTGCAGACA GGCAGCAGCT AGACTCAGAG 
CTACAAAAGG AGACCCTAGC CATCTGGCCT CACCTATCCC AGAAGATGCT GGATCTGCTT 
GTGCCCATGC CCAAAGCCTC TGACCTGACT GTGGGCAAAA TCTATGCAGC AATGATGATC 
ATGGACTACT ATAAGCAGAG TAAGGTGAAG AAGCAGAGGC AGCAGCTGGA GGAACAGAAA 
AATGCCCCCA TGTTCCAGCG CATGGAGCCT TCATCTCTGC CTCAGGAGAT CATTGCTAAT 
GCCAAAGCCC TGCCTTACCT CCAGCAGGAC CCCGTTTCAG GCCTGAGTGG CCGGAGTGGA 
TACCCTTCGA TGAGTCCACT CTCTCCCCAG GATATATTCC AGTTGGCTTG TATGGACCCC 
GCCGATGACG GACAGTTCCA AGAACGGCAG TCTCTGGTGG TGACAGACCC TAGCTCCATG 
AGACGTTCAT TTTCCACTAT TCGGGATAAG CGTTCAAATT CCTCGTGGTT GGAGGAATTC 
TCCATGGAGC GAAGCAGTGA AAATACCTAC AAGTCCCGTC GCCGGAGTTA CCACTCCTCC 
TTGCGGCTGT CAGCCCACCG CCTGAACTCT GATTCAGGCC ACAAGTCTGA CACTCACCGC 
TCAGGGGGCA GGGAGCGGGG ACGATCAAAA GAGCGAAAGC ATCTTCTCTC TCCTGATGTC 
TCCCGCTGCA ATTCAGAAGA GCGAGGGACC CAGGCTGACT GGGAGTCCCC AGAGCGCCGT 
CAATCCAGGT CACCCAGTGA GGGCAGGTCA CAGACGCCCA ACAGACAGGG CACAGGTTCC 
CTAAGTGAGA GCTCCATCCC CTCTGTCTCT GACACCAGCA CCCCAAGAAG AAGTCGTCGG 
CAGCTCCCAC CCGTCCCGCC AAAGCCCCGG CCCCTCCTTT CCTACAGCTC CCTGATTCGA 
CACGCGGGCA GCATCTCTCC ACCTGCTGAT GGAAGCGAGG AGGGCTCCCC GCTGACCTCC 
CAAGCTCTGG AGAGCAACAA TGCTTGCCTG ACCGAGTCTT CCAACTCTCC GCACCCCCAG 
CAGAGCCAAC ATGCCTCCCC ACAGCGCTAC ATCTCCGAGC CCTACTTGGC CCTGCACGAA 
GACTCCCACG CCTCAGACTG TGGTGAGGAG GAGACGCTCA CTTTCGAAGC AGCCGTGGCT 
ACTAGCCTGG GCCGTTCCAA CACCATCGGC TCAGCCCCAC CCCTGCGGCA TAGCTGGCAG 
ATGCCCAACG GGCACTATCG GCGGCGGAGG CGCGGGGGGC CTGGGCCAGG CATGATGTGT 
GGGGCTGTCA ACAACCTGCT AAGTGACACG GAAGAAGATG ACAAATGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016857739.1
CDS	426..7616
Translation	MVPEMRGLSPDRRLPEPAPCPAAGSPEPRSAGRRQRHVLALPEEEAWRPQQCALLEADASDEVGMLPASPRAASGFDNFFQVQEGEGQGWEGAMALEAGSSPFLPVSPEVMKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERERRRRHHMSVWEQRTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGLALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFEDRARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQDLRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSEPDLSCITANTDKATTESTSVTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQFQERQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNNLLSDTEEDDKC

Target ORF information:

RefSeq Version	XM_017002250.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002250.1

ORF Insert Sequence:

ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC 
CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG 
GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC 
CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC 
AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG 
CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG 
CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC 
GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG 
GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC 
ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT 
AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC 
CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT 
GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT 
TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC 
CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT 
GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG 
CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG 
AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT 
GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT 
TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT 
GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC 
CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG 
TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC 
ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC 
AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG 
ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC 
GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC 
AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC 
TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC 
TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC 
CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT 
GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT 
CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT 
TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG 
GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG 
CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC 
TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT 
GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT 
CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG 
GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA 
GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG 
AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT 
GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC 
AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAGAGGG AGCGGAGGCG CCGGCACCAC 
ATGTCCGTGT GGGAGCAGCG TACCAGCCAG CTGAGGAAGC ACATGCAGAT GTCCAGCCAG 
GAGGCCCTCA ACAGAGAGGA GGCGCCGACC ATGAACCCGC TCAACCCCCT CAACCCGCTC 
AGCTCCCTCA ACCCGCTCAA TGCCCACCCC AGCCTTTATC GGCGACCCAG GGCCATTGAG 
GGCCTGGCCC TGGGCCTGGC CCTGGAGAAG TTCGAGGAGG AGCGCATCAG CCGTGGGGGG 
TCCCTCAAGG GGGATGGAGG GGACCGATCC AGTGCCCTGG ACAACCAGAG GACCCCTTTG 
TCCCTGGGCC AGCGGGAGCC ACCATGGCTG GCCAGGCCCT GTCATGGAAA CTGTGACCCG 
ACTCAGCAGG AGGCAGGGGG AGGAGAGGCT GTGGTGACCT TTGAGGACCG GGCCAGGCAC 
AGGCAGAGCC AACGGCGCAG CCGGCATCGC CGCGTCAGGA CAGAAGGCAA GGAGTCCTCT 
TCAGCCTCCC GGAGCAGGTC TGCCAGCCAG GAACGCAGTC TGGATGAAGC CATGCCCACT 
GAAGGGGAGA AGGACCATGA GCTCAGGGGC AACCATGGTG CCAAGGAGCC AACGATCCAA 
GAAGAGAGAG CCCAGGATTT AAGGAGGACC AACAGTCTGA TGGTGTCCAG AGGCTCCGGG 
CTGGCAGGAG GCCTTGATGA GGCTGACACC CCCCTAGTCC TGCCCCATCC TGAGCTGGAA 
GTGGGGAAGC ACGTGGTGCT GACGGAGCAG GAGCCAGAAG GCAGCAGTGA GCAGGCCCTG 
CTGGGGAATG TGCAGCTAGA CATGGGCCGG GTCATCAGCC AGAGCGAGCC TGACCTCTCC 
TGCATCACGG CCAACACGGA CAAGGCCACC ACCGAGAGCA CCAGCGTCAC CGTCGCCATC 
CCCGACGTGG ACCCCTTGGT GGACTCAACC GTGGTGCACA TTAGCAACAA GACGGATGGG 
GAAGCCAGTC CCTTGAAGGA GGCAGAGATC AGAGAGGATG AGGAGGAGGT GGAGAAGAAG 
AAGCAGAAGA AGGAGAAGCG TGAGACAGGC AAAGCCATGG TGCCCCACAG CTCAATGTTC 
ATCTTCAGCA CCACCAACCC GATCCGGAGG GCCTGCCACT ACATCGTGAA CCTGCGCTAC 
TTTGAGATGT GCATCCTCCT GGTGATTGCA GCCAGCAGCA TCGCCCTGGC GGCAGAGGAC 
CCCGTCCTGA CCAACTCGGA GCGCAACAAA GTCCTGAGGT ATTTTGACTA TGTGTTCACG 
GGCGTGTTCA CCTTTGAGAT GGTTATAAAG ATGATAGACC AAGGCTTGAT CCTGCAGGAT 
GGGTCCTACT TCCGAGACTT GTGGAACATC CTGGACTTTG TGGTGGTCGT TGGCGCATTG 
GTGGCCTTTG CTCTGGCGAA CGCTTTGGGA ACCAACAAAG GACGGGACAT CAAGACCATC 
AAGTCTCTGC GGGTGCTCCG AGTTCTAAGG CCACTGAAAA CCATCAAGCG CTTGCCCAAG 
CTCAAGGCCG TCTTCGACTG CGTAGTGACC TCCTTGAAGA ATGTCTTCAA CATACTCATT 
GTGTACAAGC TCTTCATGTT CATCTTTGCT GTCATCGCAG TTCAGCTCTT CAAGGGAAAG 
TTCTTTTATT GCACGGACAG TTCCAAGGAC ACAGAGAAGG AGTGCATAGG CAACTATGTA 
GATCATGAGA AAAACAAGAT GGAGGTGAAG GGCCGGGAAT GGAAGCGCCA TGAATTCCAC 
TACGACAACA TTATCTGGGC CCTGCTGACC CTCTTCACCG TCTCCACAGG GGAAGGATGG 
CCTCAAGTTC TGCAGCACTC TGTAGATGTG ACAGAGGAAG ACCGAGGCCC AAGCCGCAGC 
AACCGCATGG AGATGTCTAT CTTTTATGTA GTCTACTTTG TGGTCTTCCC CTTCTTCTTT 
GTCAATATCT TTGTGGCTCT CATCATCATC ACCTTCCAGG AGCAAGGGGA TAAGATGATG 
GAGGAGTGCA GCCTGGAGAA GAATGAGAGG GCGTGCATCG ACTTCGCCAT CAGCGCCAAA 
CCTCTCACCC GCTACATGCC GCAGAACAGA CACACCTTCC AGTACCGCGT GTGGCACTTT 
GTGGTGTCTC CGTCCTTTGA GTACACCATT ATGGCCATGA TCGCCTTGAA TACTGTTGTG 
CTGATGATGA AGTATTATTC TGCTCCCTGT ACCTATGAGC TGGCCCTGAA GTACCTGAAT 
ATCGCCTTCA CCATGGTGTT TTCCCTGGAA TGTGTCCTGA AGGTCATCGC TTTTGGCTTT 
TTGAACTATT TCCGAGACAC CTGGAATATC TTTGACTTCA TCACCGTGAT TGGCAGTATC 
ACAGAAATTA TCCTGACAGA CAGCAAGCTG GTGAACACCA GTGGCTTCAA TATGAGCTTT 
CTGAAGCTCT TCCGAGCTGC CCGCCTCATA AAGCTCCTGC GTCAGGGCTA TACCATACGC 
ATTTTGCTGT GGACCTTTGT GCAGTCCTTT AAGGCCCTCC CTTATGTCTG CCTTTTAATT 
GCCATGCTTT TCTTCATTTA TGCCATCATT GGGATGCAGG TATTTGGAAA CATAAAATTA 
GACGAGGAGA GTCACATCAA CCGGCACAAC AACTTCCGGA GTTTCTTTGG GTCCCTAATG 
CTACTCTTCA GGAGTGCCAC AGGTGAGGCC TGGCAGGAGA TTATGCTGTC ATGCCTTGGG 
GAGAAGGGCT GTGAGCCTGA CACCACCGCA CCATCAGGGC AGAACGAGAA CGAACGCTGC 
GGCACCGATC TGGCCTACGT GTACTTTGTC TCCTTCATCT TCTTCTGCTC CTTCTTGATG 
CTCAACCTGT TTGTGGCCGT CATCATGGAC AACTTTGAGT ACCTGACTCG GGACTCCTCC 
ATCCTGGGGC CTCACCACTT GGACGAGTTT GTCCGCGTCT GGGCAGAATA TGACCGAGCA 
GCATGTGGCC GCATCCATTA CACTGAGATG TATGAAATGC TGACTCTCAT GTCACCTCCG 
CTAGGCCTCG GCAAGAGATG TCCCTCCAAA GTGGCATATA AGAGGTTGGT CCTGATGAAC 
ATGCCAGTAG CTGAGGACAT GACGGTCCAC TTCACCTCCA CACTTATGGC TCTGATCCGG 
ACAGCTCTGG ACATTAAAAT TGCCAAAGGT GGTGCAGACA GGCAGCAGCT AGACTCAGAG 
CTACAAAAGG AGACCCTAGC CATCTGGCCT CACCTATCCC AGAAGATGCT GGATCTGCTT 
GTGCCCATGC CCAAAGCCTC TGACCTGACT GTGGGCAAAA TCTATGCAGC AATGATGATC 
ATGGACTACT ATAAGCAGAG TAAGGTGAAG AAGCAGAGGC AGCAGCTGGA GGAACAGAAA 
AATGCCCCCA TGTTCCAGCG CATGGAGCCT TCATCTCTGC CTCAGGAGAT CATTGCTAAT 
GCCAAAGCCC TGCCTTACCT CCAGCAGGAC CCCGTTTCAG GCCTGAGTGG CCGGAGTGGA 
TACCCTTCGA TGAGTCCACT CTCTCCCCAG GATATATTCC AGTTGGCTTG TATGGACCCC 
GCCGATGACG GACAGTTCCA AGAACGGCAG TCTCTGGTGG TGACAGACCC TAGCTCCATG 
AGACGTTCAT TTTCCACTAT TCGGGATAAG CGTTCAAATT CCTCGTGGTT GGAGGAATTC 
TCCATGGAGC GAAGCAGTGA AAATACCTAC AAGTCCCGTC GCCGGAGTTA CCACTCCTCC 
TTGCGGCTGT CAGCCCACCG CCTGAACTCT GATTCAGGCC ACAAGTCTGA CACTCACCGC 
TCAGGGGGCA GGGAGCGGGG ACGATCAAAA GAGCGAAAGC ATCTTCTCTC TCCTGATGTC 
TCCCGCTGCA ATTCAGAAGA GCGAGGGACC CAGGCTGACT GGGAGTCCCC AGAGCGCCGT 
CAATCCAGGT CACCCAGTGA GGGCAGGTCA CAGACGCCCA ACAGACAGGG CACAGGTTCC 
CTAAGTGAGA GCTCCATCCC CTCTGTCTCT GACACCAGCA CCCCAAGAAG AAGTCGTCGG 
CAGCTCCCAC CCGTCCCGCC AAAGCCCCGG CCCCTCCTTT CCTACAGCTC CCTGATTCGA 
CACGCGGGCA GCATCTCTCC ACCTGCTGAT GGAAGCGAGG AGGGCTCCCC GCTGACCTCC 
CAAGCTCTGG AGAGCAACAA TGCTTGCCTG ACCGAGTCTT CCAACTCTCC GCACCCCCAG 
CAGAGCCAAC ATGCCTCCCC ACAGCGCTAC ATCTCCGAGC CCTACTTGGC CCTGCACGAA 
GACTCCCACG CCTCAGACTG TGGTGAGGAG GAGACGCTCA CTTTCGAAGC AGCCGTGGCT 
ACTAGCCTGG GCCGTTCCAA CACCATCGGC TCAGCCCCAC CCCTGCGGCA TAGCTGGCAG 
ATGCCCAACG GGCACTATCG GCGGCGGAGG CGCGGGGGGC CTGGGCCAGG CATGATGTGT 
GGGGCTGTCA ACAACCTGCT AAGTGACACG GAAGAAGATG ACAAATGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CACNA1E
Entrez Gene ID	777
Full Name	calcium voltage-gated channel subunit alpha1 E
Synonyms	BII,CACH6,CACNL1A6,Cav2.3,gm139
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 E Names voltage-dependent R-type calcium channel subunit alpha-1E brain calcium channel II calcium channel, L type, alpha-1 polypeptide calcium channel, R type, alpha-1 polypeptide voltage-dependent calcium channel alpha 1E subunit voltage-gated calcium channel alpha subunit Cav2.3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q25.3
Summary	Voltage-dependent calcium channels are multisubunit complexes consisting of alpha-1, alpha-2, beta, and delta subunits in a 1:1:1:1 ratio. These channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. This gene encodes the alpha-1E subunit of the R-type calcium channels, which belong to the 'high-voltage activated' group that maybe involved in the modulation of firing patterns of neurons important for information processing. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011].
Disorder MIM:	601013

Rattus norvegicus (Norway rat)	Cacna1e	NP_062167.2
Xenopus tropicalis (tropical clawed frog)	cacna1e	XP_002937409.2
Homo sapiens (human)	CACNA1E	NP_001192222.1
Bos taurus (cattle)	CACNA1E	NP_001178052.1
Mus musculus (house mouse)	Cacna1e	NP_033912.2
Gallus gallus (chicken)	CACNA1E	XP_422255.4
Pan troglodytes (chimpanzee)	CACNA1E	XP_003949701.1
	CACNA1E	XP_001110352.2
Canis lupus familiaris (dog)	CACNA1E	XP_547425.4
Danio rerio (zebrafish)	LOC564274	XP_001920777.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CACNA1E cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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