TNS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TNS1
Entrez Gene ID	7145
Full Name	tensin 1
Synonyms	MST091,MST122,MST127,MSTP091,MSTP122,MSTP127,MXRA6,PPP1R155,TNS
General protein information	Preferred Names tensin 1 Names tensin-1 Matrix-remodelling-associated protein 6 matrix-remodelling associated 6 protein phosphatase 1, regulatory subunit 155
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q35
Summary	The protein encoded by this gene localizes to focal adhesions, regions of the plasma membrane where the cell attaches to the extracellular matrix. This protein crosslinks actin filaments and contains a Src homology 2 (SH2) domain, which is often found in molecules involved in signal transduction. This protein is a substrate of calpain II. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015].
Disorder MIM:	600076

mRNA and Protein(s)

mRNA	Protein	Name
XM_017004813.1	XP_016860302.1	tensin-1 isoform X15
XM_017004810.1	XP_016860299.1	tensin-1 isoform X12
XM_017004803.1	XP_016860292.1	tensin-1 isoform X3
XM_011511713.1	XP_011510015.1	tensin-1 isoform X6
XM_024453071.1	XP_024308839.1	tensin-1 isoform X4
XM_024453075.1	XP_024308843.1	tensin-1 isoform X8
XM_024453078.1	XP_024308846.1	tensin-1 isoform X11
XM_024453079.1	XP_024308847.1	tensin-1 isoform X12
XM_024453080.1	XP_024308848.1	tensin-1 isoform X13
XM_024453083.1	XP_024308851.1	tensin-1 isoform X15
XM_011511723.2	XP_011510025.1	tensin-1 isoform X16
XM_017004813.2	XP_016860302.1	tensin-1 isoform X17
XM_017004816.2	XP_016860305.1	tensin-1 isoform X19
XM_017004816.1	XP_016860305.1	tensin-1 isoform X17
XM_017004817.1	XP_016860306.1	tensin-1 isoform X18
XM_017004808.1	XP_016860297.1	tensin-1 isoform X11
XM_024453074.1	XP_024308842.1	tensin-1 isoform X7
XM_024453077.1	XP_024308845.1	tensin-1 isoform X10
XM_017004811.1	XP_016860300.1	tensin-1 isoform X11
XM_017004812.1	XP_016860301.1	tensin-1 isoform X11
XM_017004815.1	XP_016860304.1	tensin-1 isoform X18
XM_017004805.1	XP_016860294.1	tensin-1 isoform X8
XM_017004806.1	XP_016860295.1	tensin-1 isoform X9
XM_017004804.1	XP_016860293.1	tensin-1 isoform X4
XM_011511712.1	XP_011510014.1	tensin-1 isoform X5
NM_001308022.1	NP_001294951.1	tensin-1 isoform 2
NM_001308023.1	NP_001294952.1	tensin-1 isoform 3
XM_024453084.1	XP_024308852.1	tensin-1 isoform X20
XM_017004809.1	XP_016860298.1	tensin-1 isoform X12
XM_017004801.1	XP_016860290.1	tensin-1 isoform X1
XM_011511728.1	XP_011510030.1	tensin-1 isoform X19
NM_022648.5	NP_072174.3	tensin-1 isoform 1
XM_024453070.1	XP_024308838.1	tensin-1 isoform X3
XM_024453081.1	XP_024308849.1	tensin-1 isoform X14
XM_011511715.1	XP_011510017.1	tensin-1 isoform X7
XM_017004802.1	XP_016860291.1	tensin-1 isoform X2
XM_017004807.1	XP_016860296.1	tensin-1 isoform X10
NM_022648.6	NP_072174.3	tensin-1 isoform 1
XM_024453068.1	XP_024308836.1	tensin-1 isoform X1
XM_024453069.1	XP_024308837.1	tensin-1 isoform X2
XM_024453072.1	XP_024308840.1	tensin-1 isoform X5
XM_024453073.1	XP_024308841.1	tensin-1 isoform X6
XM_024453076.1	XP_024308844.1	tensin-1 isoform X9
XM_024453082.1	XP_024308850.1	tensin-1 isoform X14
XM_017004814.1	XP_016860303.1	tensin-1 isoform X18

Pathways from BioSystems

Homologies

	LOC696960	XP_002808070.1
Bos taurus (cattle)	TNS1	NP_777191.1
Danio rerio (zebrafish)	tns1	XP_003199292.2
Xenopus tropicalis (tropical clawed frog)	tns1	XP_004918285.1
Homo sapiens (human)	TNS1	NP_072174.3
Pan troglodytes (chimpanzee)	TNS1	XP_516080.3
Mus musculus (house mouse)	Tns1	NP_082160.3
Canis lupus familiaris (dog)	TNS1	XP_005640681.1
Gallus gallus (chicken)	TNS1	NP_990786.1
Rattus norvegicus (Norway rat)	Tns1	NP_001178739.1

Gene Ontology
Bibliography

Related articles in PubMed

Hic-5 regulates fibrillar adhesion formation to control tumor extracellular matrix remodeling through interaction with tensin1.
Goreczny GJ, Forsythe IJ, Turner CE
Oncogene(2018 Jan)

Tensin 1 Is Essential for Myofibroblast Differentiation and Extracellular Matrix Formation.
Bernau K, Torr EE, Evans MD, Aoki JK, Ngam CR, Sandbo N
American journal of respiratory cell and molecular biology56(4)465-476(2017 Apr)

MicroRNA-548j functions as a metastasis promoter in human breast cancer by targeting Tensin1.
Zhan Y, Liang X, Li L, Wang B, Ding F, Li Y, Wang X, Zhan Q, Liu Z
Molecular oncology10(6)838-49(2016 06)

Identification of Tyrosine-Phosphorylated Proteins Upregulated during Epithelial-Mesenchymal Transition Induced with TGF-?.
Okayama A, Miyagi Y, Oshita F, Ito H, Nakayama H, Nishi M, Kurata Y, Kimura Y, Ryo A, Hirano H
Journal of proteome research14(10)4127-36(2015 Oct)

Meta-analysis of genome-wide association studies of adult height in East Asians identifies 17 novel loci.
He M, Xu M, Zhang B, Liang J, Chen P, Lee JY, Johnson TA, Li H, Yang X, Dai J, Liang L, Gui L, Qi Q, Huang J, Li Y, Adair LS, Aung T, Cai Q, Cheng CY, Cho MC, Cho YS, Chu M, Cui B, Gao YT, Go MJ, Gu D, Gu W, Guo H, Hao Y, Hong J, Hu Z, Hu Y, Huang J, Hwang JY, Ikram MK, Jin G, Kang DH, Khor CC, Kim BJ, Kim HT, Kubo M, Lee J, Lee J, Lee NR, Li R, Li J, Liu J, Longe J, Lu W, Lu X, Miao X, Okada Y, Ong RT, Qiu G, Seielstad M, Sim X, Song H, Takeuchi F, Tanaka T, Taylor PR, Wang L, Wang W, Wang Y, Wu C, Wu Y, Xiang YB, Yamamoto K, Yang H, Liao M, Yokota M, Young T, Zhang X, Kato N, Wang QK, Zheng W, Hu FB, Lin D, Shen H, Teo YY, Mo Z, Wong TY, Lin X, Mohlke KL, Ning G, Tsunoda T, Han BG, Shu XO, Tai ES, Wu T, Qi L
Human molecular genetics24(6)1791-800(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TNS1 plays an essential role in TGF-beta-induced myofibroblast differentiation.
Title: Tensin 1 Is Essential for Myofibroblast Differentiation and Extracellular Matrix Formation.

miR-548j functions as a metastasis-promoting miRNA to regulate breast cancer cell invasion and metastasis by targeting Tensin1 and activating Cdc42.
Title: MicroRNA-548j functions as a metastasis promoter in human breast cancer by targeting Tensin1.

TNS1, MET, and TRKC tyrosine-phosphorylated proteins are upregulated during epithelial-mesenchymal transition induced with TGF-beta, and predict the outcome in lung adenocarcinoma patients.
Title: Identification of Tyrosine-Phosphorylated Proteins Upregulated during Epithelial-Mesenchymal Transition Induced with TGF-?.

TNS1 polymorphism associated with COPD risk and lung function decline
Title: Effect of five genetic variants associated with lung function on the risk of chronic obstructive lung disease, and their joint effects on lung function.

data demonstrate that tensin1 is extensively phosphorylated on Ser/Thr residues in cells and phosphorylation by p38 MAPK regulates the specificity of the tensin1 Src homology 2 domain for binding to different proteins
Title: Comprehensive analysis of phosphorylation sites in Tensin1 reveals regulation by p38MAPK.

The following TNS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28154	NM_022648.6 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu28154	XM_024453084.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu61093	XM_011511728.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu89845	XM_017004816.2 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu89846	XM_017004817.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu89844	XM_017004815.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu89844	XM_017004814.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu104939	XM_024453068.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104940	XM_024453069.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104941	XM_024453071.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104942	XM_024453073.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104943	XM_024453074.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104944	XM_024453075.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104945	XM_024453076.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104947	XM_024453080.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu104948	XM_024453083.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu61082	XM_024453070.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89834	XM_017004801.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89835	XM_024453082.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89835	XM_024453081.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89835	XM_017004802.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89836	XM_017004803.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89837	XM_017004805.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89837	XM_024453072.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89838	XM_017004806.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89839	XM_017004807.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89840	XM_024453077.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89840	XM_017004808.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89841	XM_017004810.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89841	XM_017004809.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89842	XM_017004811.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89842	XM_024453078.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89842	XM_017004812.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89843	XM_017004813.2 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu61088	NM_001308023.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61094	NM_001308022.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61080	XM_011511712.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61081	XM_011511713.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61083	XM_011511715.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61087	XM_017004804.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu61088	XM_011511723.2 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu104946	XM_024453079.1 Latest version!	Homo sapiens tensin 1 (TNS1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OHu89843	XM_017004813.1	Homo sapiens tensin 1 (TNS1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu89845	XM_017004816.1	Homo sapiens tensin 1 (TNS1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu28154	NM_022648.5	Homo sapiens tensin 1 (TNS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNS1 ( NM_022648.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_072174.3 Homo sapiens tensin 1 (TNS1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28154
Clone ID Related Accession (Same CDS sequence)	XM_024453084.1 , NM_022648.5 , NM_022648.6
Accession Version	NM_022648.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	tensin-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL706926.1, AF225896.2, BC126910.1, BC051304.1, AB209238.1, AC009469.4 and BQ045472.1. On Apr 21, 2015 this sequence version replaced NM_022648.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and Northern Blot. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC140942.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGGGGTACAG 
GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCCCCTCTGC GGAGGCGGGC GGCCAGTGAT 
GGACAGTATG AGAACCAGTC TCCAGAAGCC ACATCCCCTC GTAGCCCTGG GGTTCGCTCC 
CCTGTCCAGT GTGTCTCCCC GGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGG 
CCCAAAGAGC CCCATTTGCA CAGCTACAAG GAGGCCTTCG AGGAGATGGA GGGAACCTCC 
CCGAGCAGCC CACCACCCAG TGGGGTGCGG TCCCCCCCGG GTCTGGCCAA GACACCCCTG 
TCTGCTCTGG GCCTGAAACC TCACAACCCA GCGGACATCC TGTTGCACCC CACAGGAGTT 
ACCAGAAGAC GGATCCAGCC AGAGGAAGAT GAGGGGAAGG TGGTGGTCAG GCTGTCAGAA 
GAGCCCCGGA GCTATGTGGA GTCTGTGGCA CGGACAGCGG TGGCTGGACC CCGAGCTCAG 
GACTCTGAGC CCAAGAGCTT TAGTGCTCCA GCCACCCAGG CCTATGGCCA TGAGATACCC 
CTGAGGAACG GGACCCTGGG TGGCTCCTTT GTCTCCCCCA GCCCCCTCTC CACCAGCAGC 
CCCATCCTCA GTGCTGACAG CACTTCAGTG GGGAGTTTCC CGTCGGGAGA GAGCAGTGAC 
CAGGGTCCCC GGACGCCCAC CCAGCCTCTG TTGGAGTCTG GCTTCCGCTC AGGCAGCCTG 
GGACAGCCCA GCCCGTCTGC CCAGAGAAAC TACCAGAGCT CTTCTCCTCT CCCGACTGTG 
GGCAGTAGCT ACAGCAGCCC CGACTACTCA CTTCAGCATT TCAGCTCCTC TCCGGAAAGC 
CAGGCTCGAG CTCAGTTCAG TGTGGCTGGC GTCCACACGG TGCCTGGGAG CCCTCAGGCG 
CGCCACAGAA CAGTGGGCAC CAACACTCCC CCTAGTCCTG GCTTCGGCTG GCGGGCCATC 
AATCCCAGCA TGGCTGCCCC CAGCAGTCCC AGTTTGAGCC ATCACCAGAT GATGGGTCCA 
CCAGGCACTG GCTTCCATGG TAGCACTGTC TCCAGCCCCC AGAGCAGTGC AGCGACCACC 
CCGGGGAGCC CCAGCCTGTG TCGGCACCCA GCAGGGGTCT ACCAGGTTTC TGGCCTCCAC 
AACAAAGTGG CCACCACCCC GGGGAGTCCC AGCCTGGGCC GGCACCCTGG GGCTCACCAA 
GGCAACCTGG CCTCCGGTCT TCATAGCAAT GCAATAGCCA GCCCTGGAAG CCCCAGCCTG 
GGCCGTCACC TCGGAGGGTC TGGATCTGTG GTTCCCGGCA GCCCCTGCTT GGACCGGCAT 
GTGGCCTATG GTGGCTATTC TACCCCGGAG GATCGGAGAC CCACACTGTC CCGGCAGAGC 
AGTGCCTCTG GCTACCAGGC TCCTTCCACG CCCTCCTTCC CTGTCTCCCC TGCCTACTAC 
CCTGGCCTGA GCAGCCCTGC CACCTCCCCG TCACCAGACT CCGCAGCCTT CCGGCAAGGG 
AGCCCAACAC CAGCCTTGCC AGAGAAGCGA AGGATGTCAG TGGGAGACCG GGCAGGCAGC 
CTCCCCAACT ATGCCACCAT CAATGGGAAG GTGTCTTCGC CTGTCGCCAG CGGCATGTCC 
AGTCCCAGTG GGGGCAGCAC CGTCTCCTTC TCCCACACTC TGCCCGACTT CTCCAAGTAC 
TCCATGCCAG ACAACAGCCC GGAGACGCGG GCTAAAGTGA AGTTTGTCCA GGACACTTCT 
AAGTATTGGT ACAAGCCTGA GATCTCCAGG GAGCAGGCCA TCGCGCTCCT CAAGGACCAG 
GAGCCGGGGG CCTTCATCAT CCGCGACAGT CACTCCTTCC GAGGCGCGTA CGGGCTGGCC 
ATGAAGGTGT CTTCGCCACC TCCAACCATC ATGCAGCAGA ATAAAAAAGG AGACATGACC 
CATGAGCTGG TCAGGCATTT TCTGATAGAG ACTGGCCCCA GAGGAGTCAA GCTCAAGGGC 
TGCCCCAATG AGCCAAACTT CGGATCGCTG TCTGCCCTGG TCTACCAGCA CTCCATCATC 
CCATTGGCCC TGCCTTGCAA GCTGGTCATT CCAAACCGAG ACCCCACAGA TGAATCGAAA 
GATAGCTCCG GCCCTGCCAA CTCAACTGCA GACCTGCTGA AACAAGGGGC AGCCTGCAAT 
GTGCTCTTCG TCAACTCTGT GGACATGGAG TCACTCACTG GGCCACAGGC CATCTCTAAA 
GCCACATCTG AGACGTTGGC TGCAGACCCC ACGCCAGCTG CCACCATCGT TCACTTCAAA 
GTCTCTGCCC AGGGAATCAC TCTGACTGAC AACCAGAGAA AGCTCTTTTT CAGACGCCAC 
TACCCTCTCA ACACTGTCAC CTTCTGTGAC CTGGATCCAC AGGAAAGAAA GTGGATGAAA 
ACAGAGGGTG GTGCCCCTGC TAAGCTCTTC GGCTTCGTGG CCCGGAAGCA GGGCAGCACC 
ACGGACAACG CCTGCCACCT CTTTGCTGAG CTTGACCCCA ACCAGCCGGC CTCTGCCATC 
GTCAACTTCG TCTCCAAGGT CATGCTGAAT GCCGGCCAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072174.3
CDS	399..5606
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	NM_022648.5
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022648.5

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGGGGTACAG 
GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCCCCTCTGC GGAGGCGGGC GGCCAGTGAT 
GGACAGTATG AGAACCAGTC TCCAGAAGCC ACATCCCCTC GTAGCCCTGG GGTTCGCTCC 
CCTGTCCAGT GTGTCTCCCC GGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGG 
CCCAAAGAGC CCCATTTGCA CAGCTACAAG GAGGCCTTCG AGGAGATGGA GGGAACCTCC 
CCGAGCAGCC CACCACCCAG TGGGGTGCGG TCCCCCCCGG GTCTGGCCAA GACACCCCTG 
TCTGCTCTGG GCCTGAAACC TCACAACCCA GCGGACATCC TGTTGCACCC CACAGGAGTT 
ACCAGAAGAC GGATCCAGCC AGAGGAAGAT GAGGGGAAGG TGGTGGTCAG GCTGTCAGAA 
GAGCCCCGGA GCTATGTGGA GTCTGTGGCA CGGACAGCGG TGGCTGGACC CCGAGCTCAG 
GACTCTGAGC CCAAGAGCTT TAGTGCTCCA GCCACCCAGG CCTATGGCCA TGAGATACCC 
CTGAGGAACG GGACCCTGGG TGGCTCCTTT GTCTCCCCCA GCCCCCTCTC CACCAGCAGC 
CCCATCCTCA GTGCTGACAG CACTTCAGTG GGGAGTTTCC CGTCGGGAGA GAGCAGTGAC 
CAGGGTCCCC GGACGCCCAC CCAGCCTCTG TTGGAGTCTG GCTTCCGCTC AGGCAGCCTG 
GGACAGCCCA GCCCGTCTGC CCAGAGAAAC TACCAGAGCT CTTCTCCTCT CCCGACTGTG 
GGCAGTAGCT ACAGCAGCCC CGACTACTCA CTTCAGCATT TCAGCTCCTC TCCGGAAAGC 
CAGGCTCGAG CTCAGTTCAG TGTGGCTGGC GTCCACACGG TGCCTGGGAG CCCTCAGGCG 
CGCCACAGAA CAGTGGGCAC CAACACTCCC CCTAGTCCTG GCTTCGGCTG GCGGGCCATC 
AATCCCAGCA TGGCTGCCCC CAGCAGTCCC AGTTTGAGCC ATCACCAGAT GATGGGTCCA 
CCAGGCACTG GCTTCCATGG TAGCACTGTC TCCAGCCCCC AGAGCAGTGC AGCGACCACC 
CCGGGGAGCC CCAGCCTGTG TCGGCACCCA GCAGGGGTCT ACCAGGTTTC TGGCCTCCAC 
AACAAAGTGG CCACCACCCC GGGGAGTCCC AGCCTGGGCC GGCACCCTGG GGCTCACCAA 
GGCAACCTGG CCTCCGGTCT TCATAGCAAT GCAATAGCCA GCCCTGGAAG CCCCAGCCTG 
GGCCGTCACC TCGGAGGGTC TGGATCTGTG GTTCCCGGCA GCCCCTGCTT GGACCGGCAT 
GTGGCCTATG GTGGCTATTC TACCCCGGAG GATCGGAGAC CCACACTGTC CCGGCAGAGC 
AGTGCCTCTG GCTACCAGGC TCCTTCCACG CCCTCCTTCC CTGTCTCCCC TGCCTACTAC 
CCTGGCCTGA GCAGCCCTGC CACCTCCCCG TCACCAGACT CCGCAGCCTT CCGGCAAGGG 
AGCCCAACAC CAGCCTTGCC AGAGAAGCGA AGGATGTCAG TGGGAGACCG GGCAGGCAGC 
CTCCCCAACT ATGCCACCAT CAATGGGAAG GTGTCTTCGC CTGTCGCCAG CGGCATGTCC 
AGTCCCAGTG GGGGCAGCAC CGTCTCCTTC TCCCACACTC TGCCCGACTT CTCCAAGTAC 
TCCATGCCAG ACAACAGCCC GGAGACGCGG GCTAAAGTGA AGTTTGTCCA GGACACTTCT 
AAGTATTGGT ACAAGCCTGA GATCTCCAGG GAGCAGGCCA TCGCGCTCCT CAAGGACCAG 
GAGCCGGGGG CCTTCATCAT CCGCGACAGT CACTCCTTCC GAGGCGCGTA CGGGCTGGCC 
ATGAAGGTGT CTTCGCCACC TCCAACCATC ATGCAGCAGA ATAAAAAAGG AGACATGACC 
CATGAGCTGG TCAGGCATTT TCTGATAGAG ACTGGCCCCA GAGGAGTCAA GCTCAAGGGC 
TGCCCCAATG AGCCAAACTT CGGATCGCTG TCTGCCCTGG TCTACCAGCA CTCCATCATC 
CCATTGGCCC TGCCTTGCAA GCTGGTCATT CCAAACCGAG ACCCCACAGA TGAATCGAAA 
GATAGCTCCG GCCCTGCCAA CTCAACTGCA GACCTGCTGA AACAAGGGGC AGCCTGCAAT 
GTGCTCTTCG TCAACTCTGT GGACATGGAG TCACTCACTG GGCCACAGGC CATCTCTAAA 
GCCACATCTG AGACGTTGGC TGCAGACCCC ACGCCAGCTG CCACCATCGT TCACTTCAAA 
GTCTCTGCCC AGGGAATCAC TCTGACTGAC AACCAGAGAA AGCTCTTTTT CAGACGCCAC 
TACCCTCTCA ACACTGTCAC CTTCTGTGAC CTGGATCCAC AGGAAAGAAA GTGGATGAAA 
ACAGAGGGTG GTGCCCCTGC TAAGCTCTTC GGCTTCGTGG CCCGGAAGCA GGGCAGCACC 
ACGGACAACG CCTGCCACCT CTTTGCTGAG CTTGACCCCA ACCAGCCGGC CTCTGCCATC 
GTCAACTTCG TCTCCAAGGT CATGCTGAAT GCCGGCCAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( NM_022648.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_072174.3 Homo sapiens tensin 1 (TNS1), transcript variant 1, mRNA.

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CloneID	OHu28154
Clone ID Related Accession (Same CDS sequence)	XM_024453084.1 , NM_022648.5 , NM_022648.6
Accession Version	NM_022648.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	tensin-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL706926.1, AF225896.2, BC126910.1, BC051304.1, AB209238.1, AC009469.4 and BQ045472.1. On Jun 2, 2019 this sequence version replaced NM_022648.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and Northern Blot. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC140942.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGGGGTACAG 
GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCCCCTCTGC GGAGGCGGGC GGCCAGTGAT 
GGACAGTATG AGAACCAGTC TCCAGAAGCC ACATCCCCTC GTAGCCCTGG GGTTCGCTCC 
CCTGTCCAGT GTGTCTCCCC GGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGG 
CCCAAAGAGC CCCATTTGCA CAGCTACAAG GAGGCCTTCG AGGAGATGGA GGGAACCTCC 
CCGAGCAGCC CACCACCCAG TGGGGTGCGG TCCCCCCCGG GTCTGGCCAA GACACCCCTG 
TCTGCTCTGG GCCTGAAACC TCACAACCCA GCGGACATCC TGTTGCACCC CACAGGAGTT 
ACCAGAAGAC GGATCCAGCC AGAGGAAGAT GAGGGGAAGG TGGTGGTCAG GCTGTCAGAA 
GAGCCCCGGA GCTATGTGGA GTCTGTGGCA CGGACAGCGG TGGCTGGACC CCGAGCTCAG 
GACTCTGAGC CCAAGAGCTT TAGTGCTCCA GCCACCCAGG CCTATGGCCA TGAGATACCC 
CTGAGGAACG GGACCCTGGG TGGCTCCTTT GTCTCCCCCA GCCCCCTCTC CACCAGCAGC 
CCCATCCTCA GTGCTGACAG CACTTCAGTG GGGAGTTTCC CGTCGGGAGA GAGCAGTGAC 
CAGGGTCCCC GGACGCCCAC CCAGCCTCTG TTGGAGTCTG GCTTCCGCTC AGGCAGCCTG 
GGACAGCCCA GCCCGTCTGC CCAGAGAAAC TACCAGAGCT CTTCTCCTCT CCCGACTGTG 
GGCAGTAGCT ACAGCAGCCC CGACTACTCA CTTCAGCATT TCAGCTCCTC TCCGGAAAGC 
CAGGCTCGAG CTCAGTTCAG TGTGGCTGGC GTCCACACGG TGCCTGGGAG CCCTCAGGCG 
CGCCACAGAA CAGTGGGCAC CAACACTCCC CCTAGTCCTG GCTTCGGCTG GCGGGCCATC 
AATCCCAGCA TGGCTGCCCC CAGCAGTCCC AGTTTGAGCC ATCACCAGAT GATGGGTCCA 
CCAGGCACTG GCTTCCATGG TAGCACTGTC TCCAGCCCCC AGAGCAGTGC AGCGACCACC 
CCGGGGAGCC CCAGCCTGTG TCGGCACCCA GCAGGGGTCT ACCAGGTTTC TGGCCTCCAC 
AACAAAGTGG CCACCACCCC GGGGAGTCCC AGCCTGGGCC GGCACCCTGG GGCTCACCAA 
GGCAACCTGG CCTCCGGTCT TCATAGCAAT GCAATAGCCA GCCCTGGAAG CCCCAGCCTG 
GGCCGTCACC TCGGAGGGTC TGGATCTGTG GTTCCCGGCA GCCCCTGCTT GGACCGGCAT 
GTGGCCTATG GTGGCTATTC TACCCCGGAG GATCGGAGAC CCACACTGTC CCGGCAGAGC 
AGTGCCTCTG GCTACCAGGC TCCTTCCACG CCCTCCTTCC CTGTCTCCCC TGCCTACTAC 
CCTGGCCTGA GCAGCCCTGC CACCTCCCCG TCACCAGACT CCGCAGCCTT CCGGCAAGGG 
AGCCCAACAC CAGCCTTGCC AGAGAAGCGA AGGATGTCAG TGGGAGACCG GGCAGGCAGC 
CTCCCCAACT ATGCCACCAT CAATGGGAAG GTGTCTTCGC CTGTCGCCAG CGGCATGTCC 
AGTCCCAGTG GGGGCAGCAC CGTCTCCTTC TCCCACACTC TGCCCGACTT CTCCAAGTAC 
TCCATGCCAG ACAACAGCCC GGAGACGCGG GCTAAAGTGA AGTTTGTCCA GGACACTTCT 
AAGTATTGGT ACAAGCCTGA GATCTCCAGG GAGCAGGCCA TCGCGCTCCT CAAGGACCAG 
GAGCCGGGGG CCTTCATCAT CCGCGACAGT CACTCCTTCC GAGGCGCGTA CGGGCTGGCC 
ATGAAGGTGT CTTCGCCACC TCCAACCATC ATGCAGCAGA ATAAAAAAGG AGACATGACC 
CATGAGCTGG TCAGGCATTT TCTGATAGAG ACTGGCCCCA GAGGAGTCAA GCTCAAGGGC 
TGCCCCAATG AGCCAAACTT CGGATCGCTG TCTGCCCTGG TCTACCAGCA CTCCATCATC 
CCATTGGCCC TGCCTTGCAA GCTGGTCATT CCAAACCGAG ACCCCACAGA TGAATCGAAA 
GATAGCTCCG GCCCTGCCAA CTCAACTGCA GACCTGCTGA AACAAGGGGC AGCCTGCAAT 
GTGCTCTTCG TCAACTCTGT GGACATGGAG TCACTCACTG GGCCACAGGC CATCTCTAAA 
GCCACATCTG AGACGTTGGC TGCAGACCCC ACGCCAGCTG CCACCATCGT TCACTTCAAA 
GTCTCTGCCC AGGGAATCAC TCTGACTGAC AACCAGAGAA AGCTCTTTTT CAGACGCCAC 
TACCCTCTCA ACACTGTCAC CTTCTGTGAC CTGGATCCAC AGGAAAGAAA GTGGATGAAA 
ACAGAGGGTG GTGCCCCTGC TAAGCTCTTC GGCTTCGTGG CCCGGAAGCA GGGCAGCACC 
ACGGACAACG CCTGCCACCT CTTTGCTGAG CTTGACCCCA ACCAGCCGGC CTCTGCCATC 
GTCAACTTCG TCTCCAAGGT CATGCTGAAT GCCGGCCAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072174.3
CDS	396..5603
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	NM_022648.6
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022648.6

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGGGGTACAG 
GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCCCCTCTGC GGAGGCGGGC GGCCAGTGAT 
GGACAGTATG AGAACCAGTC TCCAGAAGCC ACATCCCCTC GTAGCCCTGG GGTTCGCTCC 
CCTGTCCAGT GTGTCTCCCC GGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGG 
CCCAAAGAGC CCCATTTGCA CAGCTACAAG GAGGCCTTCG AGGAGATGGA GGGAACCTCC 
CCGAGCAGCC CACCACCCAG TGGGGTGCGG TCCCCCCCGG GTCTGGCCAA GACACCCCTG 
TCTGCTCTGG GCCTGAAACC TCACAACCCA GCGGACATCC TGTTGCACCC CACAGGAGTT 
ACCAGAAGAC GGATCCAGCC AGAGGAAGAT GAGGGGAAGG TGGTGGTCAG GCTGTCAGAA 
GAGCCCCGGA GCTATGTGGA GTCTGTGGCA CGGACAGCGG TGGCTGGACC CCGAGCTCAG 
GACTCTGAGC CCAAGAGCTT TAGTGCTCCA GCCACCCAGG CCTATGGCCA TGAGATACCC 
CTGAGGAACG GGACCCTGGG TGGCTCCTTT GTCTCCCCCA GCCCCCTCTC CACCAGCAGC 
CCCATCCTCA GTGCTGACAG CACTTCAGTG GGGAGTTTCC CGTCGGGAGA GAGCAGTGAC 
CAGGGTCCCC GGACGCCCAC CCAGCCTCTG TTGGAGTCTG GCTTCCGCTC AGGCAGCCTG 
GGACAGCCCA GCCCGTCTGC CCAGAGAAAC TACCAGAGCT CTTCTCCTCT CCCGACTGTG 
GGCAGTAGCT ACAGCAGCCC CGACTACTCA CTTCAGCATT TCAGCTCCTC TCCGGAAAGC 
CAGGCTCGAG CTCAGTTCAG TGTGGCTGGC GTCCACACGG TGCCTGGGAG CCCTCAGGCG 
CGCCACAGAA CAGTGGGCAC CAACACTCCC CCTAGTCCTG GCTTCGGCTG GCGGGCCATC 
AATCCCAGCA TGGCTGCCCC CAGCAGTCCC AGTTTGAGCC ATCACCAGAT GATGGGTCCA 
CCAGGCACTG GCTTCCATGG TAGCACTGTC TCCAGCCCCC AGAGCAGTGC AGCGACCACC 
CCGGGGAGCC CCAGCCTGTG TCGGCACCCA GCAGGGGTCT ACCAGGTTTC TGGCCTCCAC 
AACAAAGTGG CCACCACCCC GGGGAGTCCC AGCCTGGGCC GGCACCCTGG GGCTCACCAA 
GGCAACCTGG CCTCCGGTCT TCATAGCAAT GCAATAGCCA GCCCTGGAAG CCCCAGCCTG 
GGCCGTCACC TCGGAGGGTC TGGATCTGTG GTTCCCGGCA GCCCCTGCTT GGACCGGCAT 
GTGGCCTATG GTGGCTATTC TACCCCGGAG GATCGGAGAC CCACACTGTC CCGGCAGAGC 
AGTGCCTCTG GCTACCAGGC TCCTTCCACG CCCTCCTTCC CTGTCTCCCC TGCCTACTAC 
CCTGGCCTGA GCAGCCCTGC CACCTCCCCG TCACCAGACT CCGCAGCCTT CCGGCAAGGG 
AGCCCAACAC CAGCCTTGCC AGAGAAGCGA AGGATGTCAG TGGGAGACCG GGCAGGCAGC 
CTCCCCAACT ATGCCACCAT CAATGGGAAG GTGTCTTCGC CTGTCGCCAG CGGCATGTCC 
AGTCCCAGTG GGGGCAGCAC CGTCTCCTTC TCCCACACTC TGCCCGACTT CTCCAAGTAC 
TCCATGCCAG ACAACAGCCC GGAGACGCGG GCTAAAGTGA AGTTTGTCCA GGACACTTCT 
AAGTATTGGT ACAAGCCTGA GATCTCCAGG GAGCAGGCCA TCGCGCTCCT CAAGGACCAG 
GAGCCGGGGG CCTTCATCAT CCGCGACAGT CACTCCTTCC GAGGCGCGTA CGGGCTGGCC 
ATGAAGGTGT CTTCGCCACC TCCAACCATC ATGCAGCAGA ATAAAAAAGG AGACATGACC 
CATGAGCTGG TCAGGCATTT TCTGATAGAG ACTGGCCCCA GAGGAGTCAA GCTCAAGGGC 
TGCCCCAATG AGCCAAACTT CGGATCGCTG TCTGCCCTGG TCTACCAGCA CTCCATCATC 
CCATTGGCCC TGCCTTGCAA GCTGGTCATT CCAAACCGAG ACCCCACAGA TGAATCGAAA 
GATAGCTCCG GCCCTGCCAA CTCAACTGCA GACCTGCTGA AACAAGGGGC AGCCTGCAAT 
GTGCTCTTCG TCAACTCTGT GGACATGGAG TCACTCACTG GGCCACAGGC CATCTCTAAA 
GCCACATCTG AGACGTTGGC TGCAGACCCC ACGCCAGCTG CCACCATCGT TCACTTCAAA 
GTCTCTGCCC AGGGAATCAC TCTGACTGAC AACCAGAGAA AGCTCTTTTT CAGACGCCAC 
TACCCTCTCA ACACTGTCAC CTTCTGTGAC CTGGATCCAC AGGAAAGAAA GTGGATGAAA 
ACAGAGGGTG GTGCCCCTGC TAAGCTCTTC GGCTTCGTGG CCCGGAAGCA GGGCAGCACC 
ACGGACAACG CCTGCCACCT CTTTGCTGAG CTTGACCCCA ACCAGCCGGC CTCTGCCATC 
GTCAACTTCG TCTCCAAGGT CATGCTGAAT GCCGGCCAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453084.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308852.1 Homo sapiens tensin 1 (TNS1), transcript variant X24, mRNA.

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CloneID	OHu28154
Clone ID Related Accession (Same CDS sequence)	XM_024453084.1 , NM_022648.5 , NM_022648.6
Accession Version	XM_024453084.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGGGGTACAG 
GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCCCCTCTGC GGAGGCGGGC GGCCAGTGAT 
GGACAGTATG AGAACCAGTC TCCAGAAGCC ACATCCCCTC GTAGCCCTGG GGTTCGCTCC 
CCTGTCCAGT GTGTCTCCCC GGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGG 
CCCAAAGAGC CCCATTTGCA CAGCTACAAG GAGGCCTTCG AGGAGATGGA GGGAACCTCC 
CCGAGCAGCC CACCACCCAG TGGGGTGCGG TCCCCCCCGG GTCTGGCCAA GACACCCCTG 
TCTGCTCTGG GCCTGAAACC TCACAACCCA GCGGACATCC TGTTGCACCC CACAGGAGTT 
ACCAGAAGAC GGATCCAGCC AGAGGAAGAT GAGGGGAAGG TGGTGGTCAG GCTGTCAGAA 
GAGCCCCGGA GCTATGTGGA GTCTGTGGCA CGGACAGCGG TGGCTGGACC CCGAGCTCAG 
GACTCTGAGC CCAAGAGCTT TAGTGCTCCA GCCACCCAGG CCTATGGCCA TGAGATACCC 
CTGAGGAACG GGACCCTGGG TGGCTCCTTT GTCTCCCCCA GCCCCCTCTC CACCAGCAGC 
CCCATCCTCA GTGCTGACAG CACTTCAGTG GGGAGTTTCC CGTCGGGAGA GAGCAGTGAC 
CAGGGTCCCC GGACGCCCAC CCAGCCTCTG TTGGAGTCTG GCTTCCGCTC AGGCAGCCTG 
GGACAGCCCA GCCCGTCTGC CCAGAGAAAC TACCAGAGCT CTTCTCCTCT CCCGACTGTG 
GGCAGTAGCT ACAGCAGCCC CGACTACTCA CTTCAGCATT TCAGCTCCTC TCCGGAAAGC 
CAGGCTCGAG CTCAGTTCAG TGTGGCTGGC GTCCACACGG TGCCTGGGAG CCCTCAGGCG 
CGCCACAGAA CAGTGGGCAC CAACACTCCC CCTAGTCCTG GCTTCGGCTG GCGGGCCATC 
AATCCCAGCA TGGCTGCCCC CAGCAGTCCC AGTTTGAGCC ATCACCAGAT GATGGGTCCA 
CCAGGCACTG GCTTCCATGG TAGCACTGTC TCCAGCCCCC AGAGCAGTGC AGCGACCACC 
CCGGGGAGCC CCAGCCTGTG TCGGCACCCA GCAGGGGTCT ACCAGGTTTC TGGCCTCCAC 
AACAAAGTGG CCACCACCCC GGGGAGTCCC AGCCTGGGCC GGCACCCTGG GGCTCACCAA 
GGCAACCTGG CCTCCGGTCT TCATAGCAAT GCAATAGCCA GCCCTGGAAG CCCCAGCCTG 
GGCCGTCACC TCGGAGGGTC TGGATCTGTG GTTCCCGGCA GCCCCTGCTT GGACCGGCAT 
GTGGCCTATG GTGGCTATTC TACCCCGGAG GATCGGAGAC CCACACTGTC CCGGCAGAGC 
AGTGCCTCTG GCTACCAGGC TCCTTCCACG CCCTCCTTCC CTGTCTCCCC TGCCTACTAC 
CCTGGCCTGA GCAGCCCTGC CACCTCCCCG TCACCAGACT CCGCAGCCTT CCGGCAAGGG 
AGCCCAACAC CAGCCTTGCC AGAGAAGCGA AGGATGTCAG TGGGAGACCG GGCAGGCAGC 
CTCCCCAACT ATGCCACCAT CAATGGGAAG GTGTCTTCGC CTGTCGCCAG CGGCATGTCC 
AGTCCCAGTG GGGGCAGCAC CGTCTCCTTC TCCCACACTC TGCCCGACTT CTCCAAGTAC 
TCCATGCCAG ACAACAGCCC GGAGACGCGG GCTAAAGTGA AGTTTGTCCA GGACACTTCT 
AAGTATTGGT ACAAGCCTGA GATCTCCAGG GAGCAGGCCA TCGCGCTCCT CAAGGACCAG 
GAGCCGGGGG CCTTCATCAT CCGCGACAGT CACTCCTTCC GAGGCGCGTA CGGGCTGGCC 
ATGAAGGTGT CTTCGCCACC TCCAACCATC ATGCAGCAGA ATAAAAAAGG AGACATGACC 
CATGAGCTGG TCAGGCATTT TCTGATAGAG ACTGGCCCCA GAGGAGTCAA GCTCAAGGGC 
TGCCCCAATG AGCCAAACTT CGGATCGCTG TCTGCCCTGG TCTACCAGCA CTCCATCATC 
CCATTGGCCC TGCCTTGCAA GCTGGTCATT CCAAACCGAG ACCCCACAGA TGAATCGAAA 
GATAGCTCCG GCCCTGCCAA CTCAACTGCA GACCTGCTGA AACAAGGGGC AGCCTGCAAT 
GTGCTCTTCG TCAACTCTGT GGACATGGAG TCACTCACTG GGCCACAGGC CATCTCTAAA 
GCCACATCTG AGACGTTGGC TGCAGACCCC ACGCCAGCTG CCACCATCGT TCACTTCAAA 
GTCTCTGCCC AGGGAATCAC TCTGACTGAC AACCAGAGAA AGCTCTTTTT CAGACGCCAC 
TACCCTCTCA ACACTGTCAC CTTCTGTGAC CTGGATCCAC AGGAAAGAAA GTGGATGAAA 
ACAGAGGGTG GTGCCCCTGC TAAGCTCTTC GGCTTCGTGG CCCGGAAGCA GGGCAGCACC 
ACGGACAACG CCTGCCACCT CTTTGCTGAG CTTGACCCCA ACCAGCCGGC CTCTGCCATC 
GTCAACTTCG TCTCCAAGGT CATGCTGAAT GCCGGCCAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308852.1
CDS	343..5550
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453084.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453084.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGGGGTACAG 
GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCCCCTCTGC GGAGGCGGGC GGCCAGTGAT 
GGACAGTATG AGAACCAGTC TCCAGAAGCC ACATCCCCTC GTAGCCCTGG GGTTCGCTCC 
CCTGTCCAGT GTGTCTCCCC GGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGG 
CCCAAAGAGC CCCATTTGCA CAGCTACAAG GAGGCCTTCG AGGAGATGGA GGGAACCTCC 
CCGAGCAGCC CACCACCCAG TGGGGTGCGG TCCCCCCCGG GTCTGGCCAA GACACCCCTG 
TCTGCTCTGG GCCTGAAACC TCACAACCCA GCGGACATCC TGTTGCACCC CACAGGAGTT 
ACCAGAAGAC GGATCCAGCC AGAGGAAGAT GAGGGGAAGG TGGTGGTCAG GCTGTCAGAA 
GAGCCCCGGA GCTATGTGGA GTCTGTGGCA CGGACAGCGG TGGCTGGACC CCGAGCTCAG 
GACTCTGAGC CCAAGAGCTT TAGTGCTCCA GCCACCCAGG CCTATGGCCA TGAGATACCC 
CTGAGGAACG GGACCCTGGG TGGCTCCTTT GTCTCCCCCA GCCCCCTCTC CACCAGCAGC 
CCCATCCTCA GTGCTGACAG CACTTCAGTG GGGAGTTTCC CGTCGGGAGA GAGCAGTGAC 
CAGGGTCCCC GGACGCCCAC CCAGCCTCTG TTGGAGTCTG GCTTCCGCTC AGGCAGCCTG 
GGACAGCCCA GCCCGTCTGC CCAGAGAAAC TACCAGAGCT CTTCTCCTCT CCCGACTGTG 
GGCAGTAGCT ACAGCAGCCC CGACTACTCA CTTCAGCATT TCAGCTCCTC TCCGGAAAGC 
CAGGCTCGAG CTCAGTTCAG TGTGGCTGGC GTCCACACGG TGCCTGGGAG CCCTCAGGCG 
CGCCACAGAA CAGTGGGCAC CAACACTCCC CCTAGTCCTG GCTTCGGCTG GCGGGCCATC 
AATCCCAGCA TGGCTGCCCC CAGCAGTCCC AGTTTGAGCC ATCACCAGAT GATGGGTCCA 
CCAGGCACTG GCTTCCATGG TAGCACTGTC TCCAGCCCCC AGAGCAGTGC AGCGACCACC 
CCGGGGAGCC CCAGCCTGTG TCGGCACCCA GCAGGGGTCT ACCAGGTTTC TGGCCTCCAC 
AACAAAGTGG CCACCACCCC GGGGAGTCCC AGCCTGGGCC GGCACCCTGG GGCTCACCAA 
GGCAACCTGG CCTCCGGTCT TCATAGCAAT GCAATAGCCA GCCCTGGAAG CCCCAGCCTG 
GGCCGTCACC TCGGAGGGTC TGGATCTGTG GTTCCCGGCA GCCCCTGCTT GGACCGGCAT 
GTGGCCTATG GTGGCTATTC TACCCCGGAG GATCGGAGAC CCACACTGTC CCGGCAGAGC 
AGTGCCTCTG GCTACCAGGC TCCTTCCACG CCCTCCTTCC CTGTCTCCCC TGCCTACTAC 
CCTGGCCTGA GCAGCCCTGC CACCTCCCCG TCACCAGACT CCGCAGCCTT CCGGCAAGGG 
AGCCCAACAC CAGCCTTGCC AGAGAAGCGA AGGATGTCAG TGGGAGACCG GGCAGGCAGC 
CTCCCCAACT ATGCCACCAT CAATGGGAAG GTGTCTTCGC CTGTCGCCAG CGGCATGTCC 
AGTCCCAGTG GGGGCAGCAC CGTCTCCTTC TCCCACACTC TGCCCGACTT CTCCAAGTAC 
TCCATGCCAG ACAACAGCCC GGAGACGCGG GCTAAAGTGA AGTTTGTCCA GGACACTTCT 
AAGTATTGGT ACAAGCCTGA GATCTCCAGG GAGCAGGCCA TCGCGCTCCT CAAGGACCAG 
GAGCCGGGGG CCTTCATCAT CCGCGACAGT CACTCCTTCC GAGGCGCGTA CGGGCTGGCC 
ATGAAGGTGT CTTCGCCACC TCCAACCATC ATGCAGCAGA ATAAAAAAGG AGACATGACC 
CATGAGCTGG TCAGGCATTT TCTGATAGAG ACTGGCCCCA GAGGAGTCAA GCTCAAGGGC 
TGCCCCAATG AGCCAAACTT CGGATCGCTG TCTGCCCTGG TCTACCAGCA CTCCATCATC 
CCATTGGCCC TGCCTTGCAA GCTGGTCATT CCAAACCGAG ACCCCACAGA TGAATCGAAA 
GATAGCTCCG GCCCTGCCAA CTCAACTGCA GACCTGCTGA AACAAGGGGC AGCCTGCAAT 
GTGCTCTTCG TCAACTCTGT GGACATGGAG TCACTCACTG GGCCACAGGC CATCTCTAAA 
GCCACATCTG AGACGTTGGC TGCAGACCCC ACGCCAGCTG CCACCATCGT TCACTTCAAA 
GTCTCTGCCC AGGGAATCAC TCTGACTGAC AACCAGAGAA AGCTCTTTTT CAGACGCCAC 
TACCCTCTCA ACACTGTCAC CTTCTGTGAC CTGGATCCAC AGGAAAGAAA GTGGATGAAA 
ACAGAGGGTG GTGCCCCTGC TAAGCTCTTC GGCTTCGTGG CCCGGAAGCA GGGCAGCACC 
ACGGACAACG CCTGCCACCT CTTTGCTGAG CTTGACCCCA ACCAGCCGGC CTCTGCCATC 
GTCAACTTCG TCTCCAAGGT CATGCTGAAT GCCGGCCAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_011511728.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510030.1 Homo sapiens tensin 1 (TNS1), transcript variant X22, mRNA.

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CloneID	OHu61093
Clone ID Related Accession (Same CDS sequence)	XM_011511728.1
Accession Version	XM_011511728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC TTGTGAAGGA GAGGAAAGGG GTACAGGCTC GGGAGAAGCA GCCTGCAGAG 
CCCCCAGCCC CTCTGCGGAG GCGGGCGGCC AGTGATGGAC AGTATGAGAA CCAGTCTCCA 
GAAGCCACAT CCCCTCGTAG CCCTGGGGTT CGCTCCCCTG TCCAGTGTGT CTCCCCGGAG 
CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGGCCCA AAGAGCCCCA TTTGCACAGC 
TACAAGGAGG CCTTCGAGGA GATGGAGGGA ACCTCCCCGA GCAGCCCACC ACCCAGTGGG 
GTGCGGTCCC CCCCGGGTCT GGCCAAGACA CCCCTGTCTG CTCTGGGCCT GAAACCTCAC 
AACCCAGCGG ACATCCTGTT GCACCCCACA GGAGAGCCCC GGAGCTATGT GGAGTCTGTG 
GCACGGACAG CGGTGGCTGG ACCCCGAGCT CAGGACTCTG AGCCCAAGAG CTTTAGTGCT 
CCAGCCACCC AGGCCTATGG CCATGAGATA CCCCTGAGGA ACGGGACCCT GGGTGGCTCC 
TTTGTCTCCC CCAGCCCCCT CTCCACCAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCA 
GTGGGGAGTT TCCCGTCGGG AGAGAGCAGT GACCAGGGTC CCCGGACGCC CACCCAGCCT 
CTGTTGGAGT CTGGCTTCCG CTCAGGCAGC CTGGGACAGC CCAGCCCGTC TGCCCAGAGA 
AACTACCAGA GCTCTTCTCC TCTCCCGACT GTGGGCAGTA GCTACAGCAG CCCCGACTAC 
TCACTTCAGC ATTTCAGCTC CTCTCCGGAA AGCCAGGCTC GAGCTCAGTT CAGTGTGGCT 
GGCGTCCACA CGGTGCCTGG GAGCCCTCAG GCGCGCCACA GAACAGTGGG CACCAACACT 
CCCCCTAGTC CTGGCTTCGG CTGGCGGGCC ATCAATCCCA GCATGGCTGC CCCCAGCAGT 
CCCAGTTTGA GCCATCACCA GATGATGGGT CCACCAGGCA CTGGCTTCCA TGGTAGCACT 
GTCTCCAGCC CCCAGAGCAG TGCAGCGACC ACCCCGGGGA GCCCCAGCCT GTGTCGGCAC 
CCAGCAGGGG TCTACCAGGT TTCTGGCCTC CACAACAAAG TGGCCACCAC CCCGGGGAGT 
CCCAGCCTGG GCCGGCACCC TGGGGCTCAC CAAGGCAACC TGGCCTCCGG TCTTCATAGC 
AATGCAATAG CCAGCCCTGG AAGCCCCAGC CTGGGCCGTC ACCTCGGAGG GTCTGGATCT 
GTGGTTCCCG GCAGCCCCTG CTTGGACCGG CATGTGGCCT ATGGTGGCTA TTCTACCCCG 
GAGGATCGGA GACCCACACT GTCCCGGCAG AGCAGTGCCT CTGGCTACCA GGCTCCTTCC 
ACGCCCTCCT TCCCTGTCTC CCCTGCCTAC TACCCTGGCC TGAGCAGCCC TGCCACCTCC 
CCGTCACCAG ACTCCGCAGC CTTCCGGCAA GGGAGCCCAA CACCAGCCTT GCCAGAGAAG 
CGAAGGATGT CAGTGGGAGA CCGGGCAGGC AGCCTCCCCA ACTATGCCAC CATCAATGGG 
AAGGTGTCTT CGCCTGTCGC CAGCGGCATG TCCAGTCCCA GTGGGGGCAG CACCGTCTCC 
TTCTCCCACA CTCTGCCCGA CTTCTCCAAG TACTCCATGC CAGACAACAG CCCGGAGACG 
CGGGCTAAAG TGAAGTTTGT CCAGGACACT TCTAAGTATT GGTACAAGCC TGAGATCTCC 
AGGGAGCAGG CCATCGCGCT CCTCAAGGAC CAGGAGCCGG GGGCCTTCAT CATCCGCGAC 
AGTCACTCCT TCCGAGGCGC GTACGGGCTG GCCATGAAGG TGTCTTCGCC ACCTCCAACC 
ATCATGCAGC AGAATAAAAA AGGAGACATG ACCCATGAGC TGGTCAGGCA TTTTCTGATA 
GAGACTGGCC CCAGAGGAGT CAAGCTCAAG GGCTGCCCCA ATGAGCCAAA CTTCGGATCG 
CTGTCTGCCC TGGTCTACCA GCACTCCATC ATCCCATTGG CCCTGCCTTG CAAGCTGGTC 
ATTCCAAACC GAGACCCCAC AGATGAATCG AAAGATAGCT CCGGCCCTGC CAACTCAACT 
GCAGACCTGC TGAAACAAGG GGCAGCCTGC AATGTGCTCT TCGTCAACTC TGTGGACATG 
GAGTCACTCA CTGGGCCACA GGCCATCTCT AAAGCCACAT CTGAGACGTT GGCTGCAGAC 
CCCACGCCAG CTGCCACCAT CGTTCACTTC AAAGTCTCTG CCCAGGGAAT CACTCTGACT 
GACAACCAGA GAAAGCTCTT TTTCAGACGC CACTACCCTC TCAACACTGT CACCTTCTGT 
GACCTGGATC CACAGGAAAG AAAGTGGATG AAAACAGAGG GTGGTGCCCC TGCTAAGCTC 
TTCGGCTTCG TGGCCCGGAA GCAGGGCAGC ACCACGGACA ACGCCTGCCA CCTCTTTGCT 
GAGCTTGACC CCAACCAGCC GGCCTCTGCC ATCGTCAACT TCGTCTCCAA GGTCATGCTG 
AATGCCGGCC AAAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510030.1
CDS	4102..6642
Translation	MEQLVKERKGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_011511728.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511728.1

ORF Insert Sequence:

ATGGAGCAGC TTGTGAAGGA GAGGAAAGGG GTACAGGCTC GGGAGAAGCA GCCTGCAGAG 
CCCCCAGCCC CTCTGCGGAG GCGGGCGGCC AGTGATGGAC AGTATGAGAA CCAGTCTCCA 
GAAGCCACAT CCCCTCGTAG CCCTGGGGTT CGCTCCCCTG TCCAGTGTGT CTCCCCGGAG 
CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGGCCCA AAGAGCCCCA TTTGCACAGC 
TACAAGGAGG CCTTCGAGGA GATGGAGGGA ACCTCCCCGA GCAGCCCACC ACCCAGTGGG 
GTGCGGTCCC CCCCGGGTCT GGCCAAGACA CCCCTGTCTG CTCTGGGCCT GAAACCTCAC 
AACCCAGCGG ACATCCTGTT GCACCCCACA GGAGAGCCCC GGAGCTATGT GGAGTCTGTG 
GCACGGACAG CGGTGGCTGG ACCCCGAGCT CAGGACTCTG AGCCCAAGAG CTTTAGTGCT 
CCAGCCACCC AGGCCTATGG CCATGAGATA CCCCTGAGGA ACGGGACCCT GGGTGGCTCC 
TTTGTCTCCC CCAGCCCCCT CTCCACCAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCA 
GTGGGGAGTT TCCCGTCGGG AGAGAGCAGT GACCAGGGTC CCCGGACGCC CACCCAGCCT 
CTGTTGGAGT CTGGCTTCCG CTCAGGCAGC CTGGGACAGC CCAGCCCGTC TGCCCAGAGA 
AACTACCAGA GCTCTTCTCC TCTCCCGACT GTGGGCAGTA GCTACAGCAG CCCCGACTAC 
TCACTTCAGC ATTTCAGCTC CTCTCCGGAA AGCCAGGCTC GAGCTCAGTT CAGTGTGGCT 
GGCGTCCACA CGGTGCCTGG GAGCCCTCAG GCGCGCCACA GAACAGTGGG CACCAACACT 
CCCCCTAGTC CTGGCTTCGG CTGGCGGGCC ATCAATCCCA GCATGGCTGC CCCCAGCAGT 
CCCAGTTTGA GCCATCACCA GATGATGGGT CCACCAGGCA CTGGCTTCCA TGGTAGCACT 
GTCTCCAGCC CCCAGAGCAG TGCAGCGACC ACCCCGGGGA GCCCCAGCCT GTGTCGGCAC 
CCAGCAGGGG TCTACCAGGT TTCTGGCCTC CACAACAAAG TGGCCACCAC CCCGGGGAGT 
CCCAGCCTGG GCCGGCACCC TGGGGCTCAC CAAGGCAACC TGGCCTCCGG TCTTCATAGC 
AATGCAATAG CCAGCCCTGG AAGCCCCAGC CTGGGCCGTC ACCTCGGAGG GTCTGGATCT 
GTGGTTCCCG GCAGCCCCTG CTTGGACCGG CATGTGGCCT ATGGTGGCTA TTCTACCCCG 
GAGGATCGGA GACCCACACT GTCCCGGCAG AGCAGTGCCT CTGGCTACCA GGCTCCTTCC 
ACGCCCTCCT TCCCTGTCTC CCCTGCCTAC TACCCTGGCC TGAGCAGCCC TGCCACCTCC 
CCGTCACCAG ACTCCGCAGC CTTCCGGCAA GGGAGCCCAA CACCAGCCTT GCCAGAGAAG 
CGAAGGATGT CAGTGGGAGA CCGGGCAGGC AGCCTCCCCA ACTATGCCAC CATCAATGGG 
AAGGTGTCTT CGCCTGTCGC CAGCGGCATG TCCAGTCCCA GTGGGGGCAG CACCGTCTCC 
TTCTCCCACA CTCTGCCCGA CTTCTCCAAG TACTCCATGC CAGACAACAG CCCGGAGACG 
CGGGCTAAAG TGAAGTTTGT CCAGGACACT TCTAAGTATT GGTACAAGCC TGAGATCTCC 
AGGGAGCAGG CCATCGCGCT CCTCAAGGAC CAGGAGCCGG GGGCCTTCAT CATCCGCGAC 
AGTCACTCCT TCCGAGGCGC GTACGGGCTG GCCATGAAGG TGTCTTCGCC ACCTCCAACC 
ATCATGCAGC AGAATAAAAA AGGAGACATG ACCCATGAGC TGGTCAGGCA TTTTCTGATA 
GAGACTGGCC CCAGAGGAGT CAAGCTCAAG GGCTGCCCCA ATGAGCCAAA CTTCGGATCG 
CTGTCTGCCC TGGTCTACCA GCACTCCATC ATCCCATTGG CCCTGCCTTG CAAGCTGGTC 
ATTCCAAACC GAGACCCCAC AGATGAATCG AAAGATAGCT CCGGCCCTGC CAACTCAACT 
GCAGACCTGC TGAAACAAGG GGCAGCCTGC AATGTGCTCT TCGTCAACTC TGTGGACATG 
GAGTCACTCA CTGGGCCACA GGCCATCTCT AAAGCCACAT CTGAGACGTT GGCTGCAGAC 
CCCACGCCAG CTGCCACCAT CGTTCACTTC AAAGTCTCTG CCCAGGGAAT CACTCTGACT 
GACAACCAGA GAAAGCTCTT TTTCAGACGC CACTACCCTC TCAACACTGT CACCTTCTGT 
GACCTGGATC CACAGGAAAG AAAGTGGATG AAAACAGAGG GTGGTGCCCC TGCTAAGCTC 
TTCGGCTTCG TGGCCCGGAA GCAGGGCAGC ACCACGGACA ACGCCTGCCA CCTCTTTGCT 
GAGCTTGACC CCAACCAGCC GGCCTCTGCC ATCGTCAACT TCGTCTCCAA GGTCATGCTG 
AATGCCGGCC AAAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004816.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860305.1 Homo sapiens tensin 1 (TNS1), transcript variant X23, mRNA.

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CloneID	OHu89845
Clone ID Related Accession (Same CDS sequence)	XM_017004816.2 , XM_017004816.1
Accession Version	XM_017004816.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017004816.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCTG GGAGGAGGGG GCATGTGGCT CAATCTAAAA TGTTTGGAAT TCTAACTTTT 
GAGCTTCTAG ATCTGGGACC TGGGGGTTTC CATATTCCCT TCTTTCTTTC CCACCCACAT 
GTCTTGATTT TAGTTAGGGT GGGTGTGCCC CTTCTCTCCA AGCAAAGAGC CTCTTTGGGA 
GACAGAAAAT GCACTTCAGG GTGTGGACAA GGAGCTGACA GTGCCAGACC GGAGGAGCCC 
AAGTCACCCT GTGCTGCAGC TGCTCTTCAC CGCCAGCAGC CACTTGGCAG GGTTTGGGCT 
CCTGGAATGT TCTACATGAA GCACCCTCCC TGTCCCCACC TCTCCCGCCC TGCCCAACTC 
CCTGTAGCCT CTGATGCAGC TGCTCCAGGA TTGGAGGGAA GGAGGAAAAC AGCAGGAGAG 
GTGGTGCGAG TGAGGAGTGG AGGAAGGCAG GGATGGGCGG CTAGGAGCAG CGGAGGCAGC 
AGCAGGGCTG GTTCTGAACA CTGGGCCTGG AATTGGCAGG AAAGAATTAG GAAGAACTTT 
CTTTCCTGCC TCTTCTCACC TGATCCTAGC TGGGCAGGTG GGTATCCTCA TTCCCACTTT 
GCAGATGAGG AAACGGAGTC CCAGAAAGTC TCTAAGCCCC GGAGCTATGT GGAGTCTGTG 
GCACGGACAG CGGTGGCTGG ACCCCGAGCT CAGGACTCTG AGCCCAAGAG CTTTAGTGCT 
CCAGCCACCC AGGCCTATGG CCATGAGATA CCCCTGAGGA ACGGGACCCT GGGTGGCTCC 
TTTGTCTCCC CCAGCCCCCT CTCCACCAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCA 
GTGGGGAGTT TCCCGTCGGG AGAGAGCAGT GACCAGGGTC CCCGGACGCC CACCCAGCCT 
CTGTTGGAGT CTGGCTTCCG CTCAGGCAGC CTGGGACAGC CCAGCCCGTC TGCCCAGAGA 
AACTACCAGA GCTCTTCTCC TCTCCCGACT GTGGGCAGTA GCTACAGCAG CCCCGACTAC 
TCACTTCAGC ATTTCAGCTC CTCTCCGGAA AGCCAGGCTC GAGCTCAGTT CAGTGTGGCT 
GGCGTCCACA CGGTGCCTGG GAGCCCTCAG GCGCGCCACA GAACAGTGGG CACCAACACT 
CCCCCTAGTC CTGGCTTCGG CTGGCGGGCC ATCAATCCCA GCATGGCTGC CCCCAGCAGT 
CCCAGTTTGA GCCATCACCA GATGATGGGT CCACCAGGCA CTGGCTTCCA TGGTAGCACT 
GTCTCCAGCC CCCAGAGCAG TGCAGCGACC ACCCCGGGGA GCCCCAGCCT GTGTCGGCAC 
CCAGCAGGGG TCTACCAGGT TTCTGGCCTC CACAACAAAG TGGCCACCAC CCCGGGGAGT 
CCCAGCCTGG GCCGGCACCC TGGGGCTCAC CAAGGCAACC TGGCCTCCGG TCTTCATAGC 
AATGCAATAG CCAGCCCTGG AAGCCCCAGC CTGGGCCGTC ACCTCGGAGG GTCTGGATCT 
GTGGTTCCCG GCAGCCCCTG CTTGGACCGG CATGTGGCCT ATGGTGGCTA TTCTACCCCG 
GAGGATCGGA GACCCACACT GTCCCGGCAG AGCAGTGCCT CTGGCTACCA GGCTCCTTCC 
ACGCCCTCCT TCCCTGTCTC CCCTGCCTAC TACCCTGGCC TGAGCAGCCC TGCCACCTCC 
CCGTCACCAG ACTCCGCAGC CTTCCGGCAA GGGAGCCCAA CACCAGCCTT GCCAGAGAAG 
CGAAGGATGT CAGTGGGAGA CCGGGCAGGC AGCCTCCCCA ACTATGCCAC CATCAATGGG 
AAGGTGTCTT CGCCTGTCGC CAGCGGCATG TCCAGTCCCA GTGGGGGCAG CACCGTCTCC 
TTCTCCCACA CTCTGCCCGA CTTCTCCAAG TACTCCATGC CAGACAACAG CCCGGAGACG 
CGGGCTAAAG TGAAGTTTGT CCAGGACACT TCTAAGTATT GGTACAAGCC TGAGATCTCC 
AGGGAGCAGG CCATCGCGCT CCTCAAGGAC CAGGAGCCGG GGGCCTTCAT CATCCGCGAC 
AGTCACTCCT TCCGAGGCGC GTACGGGCTG GCCATGAAGG TGTCTTCGCC ACCTCCAACC 
ATCATGCAGC AGAATAAAAA AGGAGACATG ACCCATGAGC TGGTCAGGCA TTTTCTGATA 
GAGACTGGCC CCAGAGGAGT CAAGCTCAAG GGCTGCCCCA ATGAGCCAAA CTTCGGATCG 
CTGTCTGCCC TGGTCTACCA GCACTCCATC ATCCCATTGG CCCTGCCTTG CAAGCTGGTC 
ATTCCAAACC GAGACCCCAC AGATGAATCG AAAGATAGCT CCGGCCCTGC CAACTCAACT 
GCAGACCTGC TGAAACAAGG GGCAGCCTGC AATGTGCTCT TCGTCAACTC TGTGGACATG 
GAGTCACTCA CTGGGCCACA GGCCATCTCT AAAGCCACAT CTGAGACGTT GGCTGCAGAC 
CCCACGCCAG CTGCCACCAT CGTTCACTTC AAAGTCTCTG CCCAGGGAAT CACTCTGACT 
GACAACCAGA GAAAGCTCTT TTTCAGACGC CACTACCCTC TCAACACTGT CACCTTCTGT 
GACCTGGATC CACAGGAAAG AAAGTGGATG AAAACAGAGG GTGGTGCCCC TGCTAAGCTC 
TTCGGCTTCG TGGCCCGGAA GCAGGGCAGC ACCACGGACA ACGCCTGCCA CCTCTTTGCT 
GAGCTTGACC CCAACCAGCC GGCCTCTGCC ATCGTCAACT TCGTCTCCAA GGTCATGCTG 
AATGCCGGCC AAAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860305.1
CDS	2078..4858
Translation	MGAGRRGHVAQSKMFGILTFELLDLGPGGFHIPFFLSHPHVLILVRVGVPLLSKQRASLGDRKCTSGCGQGADSARPEEPKSPCAAAALHRQQPLGRVWAPGMFYMKHPPCPHLSRPAQLPVASDAAAPGLEGRRKTAGEVVRVRSGGRQGWAARSSGGSSRAGSEHWAWNWQERIRKNFLSCLFSPDPSWAGGYPHSHFADEETESQKVSKPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004816.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004816.2

ORF Insert Sequence:

ATGGGGGCTG GGAGGAGGGG GCATGTGGCT CAATCTAAAA TGTTTGGAAT TCTAACTTTT 
GAGCTTCTAG ATCTGGGACC TGGGGGTTTC CATATTCCCT TCTTTCTTTC CCACCCACAT 
GTCTTGATTT TAGTTAGGGT GGGTGTGCCC CTTCTCTCCA AGCAAAGAGC CTCTTTGGGA 
GACAGAAAAT GCACTTCAGG GTGTGGACAA GGAGCTGACA GTGCCAGACC GGAGGAGCCC 
AAGTCACCCT GTGCTGCAGC TGCTCTTCAC CGCCAGCAGC CACTTGGCAG GGTTTGGGCT 
CCTGGAATGT TCTACATGAA GCACCCTCCC TGTCCCCACC TCTCCCGCCC TGCCCAACTC 
CCTGTAGCCT CTGATGCAGC TGCTCCAGGA TTGGAGGGAA GGAGGAAAAC AGCAGGAGAG 
GTGGTGCGAG TGAGGAGTGG AGGAAGGCAG GGATGGGCGG CTAGGAGCAG CGGAGGCAGC 
AGCAGGGCTG GTTCTGAACA CTGGGCCTGG AATTGGCAGG AAAGAATTAG GAAGAACTTT 
CTTTCCTGCC TCTTCTCACC TGATCCTAGC TGGGCAGGTG GGTATCCTCA TTCCCACTTT 
GCAGATGAGG AAACGGAGTC CCAGAAAGTC TCTAAGCCCC GGAGCTATGT GGAGTCTGTG 
GCACGGACAG CGGTGGCTGG ACCCCGAGCT CAGGACTCTG AGCCCAAGAG CTTTAGTGCT 
CCAGCCACCC AGGCCTATGG CCATGAGATA CCCCTGAGGA ACGGGACCCT GGGTGGCTCC 
TTTGTCTCCC CCAGCCCCCT CTCCACCAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCA 
GTGGGGAGTT TCCCGTCGGG AGAGAGCAGT GACCAGGGTC CCCGGACGCC CACCCAGCCT 
CTGTTGGAGT CTGGCTTCCG CTCAGGCAGC CTGGGACAGC CCAGCCCGTC TGCCCAGAGA 
AACTACCAGA GCTCTTCTCC TCTCCCGACT GTGGGCAGTA GCTACAGCAG CCCCGACTAC 
TCACTTCAGC ATTTCAGCTC CTCTCCGGAA AGCCAGGCTC GAGCTCAGTT CAGTGTGGCT 
GGCGTCCACA CGGTGCCTGG GAGCCCTCAG GCGCGCCACA GAACAGTGGG CACCAACACT 
CCCCCTAGTC CTGGCTTCGG CTGGCGGGCC ATCAATCCCA GCATGGCTGC CCCCAGCAGT 
CCCAGTTTGA GCCATCACCA GATGATGGGT CCACCAGGCA CTGGCTTCCA TGGTAGCACT 
GTCTCCAGCC CCCAGAGCAG TGCAGCGACC ACCCCGGGGA GCCCCAGCCT GTGTCGGCAC 
CCAGCAGGGG TCTACCAGGT TTCTGGCCTC CACAACAAAG TGGCCACCAC CCCGGGGAGT 
CCCAGCCTGG GCCGGCACCC TGGGGCTCAC CAAGGCAACC TGGCCTCCGG TCTTCATAGC 
AATGCAATAG CCAGCCCTGG AAGCCCCAGC CTGGGCCGTC ACCTCGGAGG GTCTGGATCT 
GTGGTTCCCG GCAGCCCCTG CTTGGACCGG CATGTGGCCT ATGGTGGCTA TTCTACCCCG 
GAGGATCGGA GACCCACACT GTCCCGGCAG AGCAGTGCCT CTGGCTACCA GGCTCCTTCC 
ACGCCCTCCT TCCCTGTCTC CCCTGCCTAC TACCCTGGCC TGAGCAGCCC TGCCACCTCC 
CCGTCACCAG ACTCCGCAGC CTTCCGGCAA GGGAGCCCAA CACCAGCCTT GCCAGAGAAG 
CGAAGGATGT CAGTGGGAGA CCGGGCAGGC AGCCTCCCCA ACTATGCCAC CATCAATGGG 
AAGGTGTCTT CGCCTGTCGC CAGCGGCATG TCCAGTCCCA GTGGGGGCAG CACCGTCTCC 
TTCTCCCACA CTCTGCCCGA CTTCTCCAAG TACTCCATGC CAGACAACAG CCCGGAGACG 
CGGGCTAAAG TGAAGTTTGT CCAGGACACT TCTAAGTATT GGTACAAGCC TGAGATCTCC 
AGGGAGCAGG CCATCGCGCT CCTCAAGGAC CAGGAGCCGG GGGCCTTCAT CATCCGCGAC 
AGTCACTCCT TCCGAGGCGC GTACGGGCTG GCCATGAAGG TGTCTTCGCC ACCTCCAACC 
ATCATGCAGC AGAATAAAAA AGGAGACATG ACCCATGAGC TGGTCAGGCA TTTTCTGATA 
GAGACTGGCC CCAGAGGAGT CAAGCTCAAG GGCTGCCCCA ATGAGCCAAA CTTCGGATCG 
CTGTCTGCCC TGGTCTACCA GCACTCCATC ATCCCATTGG CCCTGCCTTG CAAGCTGGTC 
ATTCCAAACC GAGACCCCAC AGATGAATCG AAAGATAGCT CCGGCCCTGC CAACTCAACT 
GCAGACCTGC TGAAACAAGG GGCAGCCTGC AATGTGCTCT TCGTCAACTC TGTGGACATG 
GAGTCACTCA CTGGGCCACA GGCCATCTCT AAAGCCACAT CTGAGACGTT GGCTGCAGAC 
CCCACGCCAG CTGCCACCAT CGTTCACTTC AAAGTCTCTG CCCAGGGAAT CACTCTGACT 
GACAACCAGA GAAAGCTCTT TTTCAGACGC CACTACCCTC TCAACACTGT CACCTTCTGT 
GACCTGGATC CACAGGAAAG AAAGTGGATG AAAACAGAGG GTGGTGCCCC TGCTAAGCTC 
TTCGGCTTCG TGGCCCGGAA GCAGGGCAGC ACCACGGACA ACGCCTGCCA CCTCTTTGCT 
GAGCTTGACC CCAACCAGCC GGCCTCTGCC ATCGTCAACT TCGTCTCCAA GGTCATGCTG 
AATGCCGGCC AAAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004816.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860305.1 Homo sapiens tensin 1 (TNS1), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OHu89845
Clone ID Related Accession (Same CDS sequence)	XM_017004816.2 , XM_017004816.1
Accession Version	XM_017004816.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCTG GGAGGAGGGG GCATGTGGCT CAATCTAAAA TGTTTGGAAT TCTAACTTTT 
GAGCTTCTAG ATCTGGGACC TGGGGGTTTC CATATTCCCT TCTTTCTTTC CCACCCACAT 
GTCTTGATTT TAGTTAGGGT GGGTGTGCCC CTTCTCTCCA AGCAAAGAGC CTCTTTGGGA 
GACAGAAAAT GCACTTCAGG GTGTGGACAA GGAGCTGACA GTGCCAGACC GGAGGAGCCC 
AAGTCACCCT GTGCTGCAGC TGCTCTTCAC CGCCAGCAGC CACTTGGCAG GGTTTGGGCT 
CCTGGAATGT TCTACATGAA GCACCCTCCC TGTCCCCACC TCTCCCGCCC TGCCCAACTC 
CCTGTAGCCT CTGATGCAGC TGCTCCAGGA TTGGAGGGAA GGAGGAAAAC AGCAGGAGAG 
GTGGTGCGAG TGAGGAGTGG AGGAAGGCAG GGATGGGCGG CTAGGAGCAG CGGAGGCAGC 
AGCAGGGCTG GTTCTGAACA CTGGGCCTGG AATTGGCAGG AAAGAATTAG GAAGAACTTT 
CTTTCCTGCC TCTTCTCACC TGATCCTAGC TGGGCAGGTG GGTATCCTCA TTCCCACTTT 
GCAGATGAGG AAACGGAGTC CCAGAAAGTC TCTAAGCCCC GGAGCTATGT GGAGTCTGTG 
GCACGGACAG CGGTGGCTGG ACCCCGAGCT CAGGACTCTG AGCCCAAGAG CTTTAGTGCT 
CCAGCCACCC AGGCCTATGG CCATGAGATA CCCCTGAGGA ACGGGACCCT GGGTGGCTCC 
TTTGTCTCCC CCAGCCCCCT CTCCACCAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCA 
GTGGGGAGTT TCCCGTCGGG AGAGAGCAGT GACCAGGGTC CCCGGACGCC CACCCAGCCT 
CTGTTGGAGT CTGGCTTCCG CTCAGGCAGC CTGGGACAGC CCAGCCCGTC TGCCCAGAGA 
AACTACCAGA GCTCTTCTCC TCTCCCGACT GTGGGCAGTA GCTACAGCAG CCCCGACTAC 
TCACTTCAGC ATTTCAGCTC CTCTCCGGAA AGCCAGGCTC GAGCTCAGTT CAGTGTGGCT 
GGCGTCCACA CGGTGCCTGG GAGCCCTCAG GCGCGCCACA GAACAGTGGG CACCAACACT 
CCCCCTAGTC CTGGCTTCGG CTGGCGGGCC ATCAATCCCA GCATGGCTGC CCCCAGCAGT 
CCCAGTTTGA GCCATCACCA GATGATGGGT CCACCAGGCA CTGGCTTCCA TGGTAGCACT 
GTCTCCAGCC CCCAGAGCAG TGCAGCGACC ACCCCGGGGA GCCCCAGCCT GTGTCGGCAC 
CCAGCAGGGG TCTACCAGGT TTCTGGCCTC CACAACAAAG TGGCCACCAC CCCGGGGAGT 
CCCAGCCTGG GCCGGCACCC TGGGGCTCAC CAAGGCAACC TGGCCTCCGG TCTTCATAGC 
AATGCAATAG CCAGCCCTGG AAGCCCCAGC CTGGGCCGTC ACCTCGGAGG GTCTGGATCT 
GTGGTTCCCG GCAGCCCCTG CTTGGACCGG CATGTGGCCT ATGGTGGCTA TTCTACCCCG 
GAGGATCGGA GACCCACACT GTCCCGGCAG AGCAGTGCCT CTGGCTACCA GGCTCCTTCC 
ACGCCCTCCT TCCCTGTCTC CCCTGCCTAC TACCCTGGCC TGAGCAGCCC TGCCACCTCC 
CCGTCACCAG ACTCCGCAGC CTTCCGGCAA GGGAGCCCAA CACCAGCCTT GCCAGAGAAG 
CGAAGGATGT CAGTGGGAGA CCGGGCAGGC AGCCTCCCCA ACTATGCCAC CATCAATGGG 
AAGGTGTCTT CGCCTGTCGC CAGCGGCATG TCCAGTCCCA GTGGGGGCAG CACCGTCTCC 
TTCTCCCACA CTCTGCCCGA CTTCTCCAAG TACTCCATGC CAGACAACAG CCCGGAGACG 
CGGGCTAAAG TGAAGTTTGT CCAGGACACT TCTAAGTATT GGTACAAGCC TGAGATCTCC 
AGGGAGCAGG CCATCGCGCT CCTCAAGGAC CAGGAGCCGG GGGCCTTCAT CATCCGCGAC 
AGTCACTCCT TCCGAGGCGC GTACGGGCTG GCCATGAAGG TGTCTTCGCC ACCTCCAACC 
ATCATGCAGC AGAATAAAAA AGGAGACATG ACCCATGAGC TGGTCAGGCA TTTTCTGATA 
GAGACTGGCC CCAGAGGAGT CAAGCTCAAG GGCTGCCCCA ATGAGCCAAA CTTCGGATCG 
CTGTCTGCCC TGGTCTACCA GCACTCCATC ATCCCATTGG CCCTGCCTTG CAAGCTGGTC 
ATTCCAAACC GAGACCCCAC AGATGAATCG AAAGATAGCT CCGGCCCTGC CAACTCAACT 
GCAGACCTGC TGAAACAAGG GGCAGCCTGC AATGTGCTCT TCGTCAACTC TGTGGACATG 
GAGTCACTCA CTGGGCCACA GGCCATCTCT AAAGCCACAT CTGAGACGTT GGCTGCAGAC 
CCCACGCCAG CTGCCACCAT CGTTCACTTC AAAGTCTCTG CCCAGGGAAT CACTCTGACT 
GACAACCAGA GAAAGCTCTT TTTCAGACGC CACTACCCTC TCAACACTGT CACCTTCTGT 
GACCTGGATC CACAGGAAAG AAAGTGGATG AAAACAGAGG GTGGTGCCCC TGCTAAGCTC 
TTCGGCTTCG TGGCCCGGAA GCAGGGCAGC ACCACGGACA ACGCCTGCCA CCTCTTTGCT 
GAGCTTGACC CCAACCAGCC GGCCTCTGCC ATCGTCAACT TCGTCTCCAA GGTCATGCTG 
AATGCCGGCC AAAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860305.1
CDS	2077..4857
Translation	MGAGRRGHVAQSKMFGILTFELLDLGPGGFHIPFFLSHPHVLILVRVGVPLLSKQRASLGDRKCTSGCGQGADSARPEEPKSPCAAAALHRQQPLGRVWAPGMFYMKHPPCPHLSRPAQLPVASDAAAPGLEGRRKTAGEVVRVRSGGRQGWAARSSGGSSRAGSEHWAWNWQERIRKNFLSCLFSPDPSWAGGYPHSHFADEETESQKVSKPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004816.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004816.1

ORF Insert Sequence:

ATGGGGGCTG GGAGGAGGGG GCATGTGGCT CAATCTAAAA TGTTTGGAAT TCTAACTTTT 
GAGCTTCTAG ATCTGGGACC TGGGGGTTTC CATATTCCCT TCTTTCTTTC CCACCCACAT 
GTCTTGATTT TAGTTAGGGT GGGTGTGCCC CTTCTCTCCA AGCAAAGAGC CTCTTTGGGA 
GACAGAAAAT GCACTTCAGG GTGTGGACAA GGAGCTGACA GTGCCAGACC GGAGGAGCCC 
AAGTCACCCT GTGCTGCAGC TGCTCTTCAC CGCCAGCAGC CACTTGGCAG GGTTTGGGCT 
CCTGGAATGT TCTACATGAA GCACCCTCCC TGTCCCCACC TCTCCCGCCC TGCCCAACTC 
CCTGTAGCCT CTGATGCAGC TGCTCCAGGA TTGGAGGGAA GGAGGAAAAC AGCAGGAGAG 
GTGGTGCGAG TGAGGAGTGG AGGAAGGCAG GGATGGGCGG CTAGGAGCAG CGGAGGCAGC 
AGCAGGGCTG GTTCTGAACA CTGGGCCTGG AATTGGCAGG AAAGAATTAG GAAGAACTTT 
CTTTCCTGCC TCTTCTCACC TGATCCTAGC TGGGCAGGTG GGTATCCTCA TTCCCACTTT 
GCAGATGAGG AAACGGAGTC CCAGAAAGTC TCTAAGCCCC GGAGCTATGT GGAGTCTGTG 
GCACGGACAG CGGTGGCTGG ACCCCGAGCT CAGGACTCTG AGCCCAAGAG CTTTAGTGCT 
CCAGCCACCC AGGCCTATGG CCATGAGATA CCCCTGAGGA ACGGGACCCT GGGTGGCTCC 
TTTGTCTCCC CCAGCCCCCT CTCCACCAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCA 
GTGGGGAGTT TCCCGTCGGG AGAGAGCAGT GACCAGGGTC CCCGGACGCC CACCCAGCCT 
CTGTTGGAGT CTGGCTTCCG CTCAGGCAGC CTGGGACAGC CCAGCCCGTC TGCCCAGAGA 
AACTACCAGA GCTCTTCTCC TCTCCCGACT GTGGGCAGTA GCTACAGCAG CCCCGACTAC 
TCACTTCAGC ATTTCAGCTC CTCTCCGGAA AGCCAGGCTC GAGCTCAGTT CAGTGTGGCT 
GGCGTCCACA CGGTGCCTGG GAGCCCTCAG GCGCGCCACA GAACAGTGGG CACCAACACT 
CCCCCTAGTC CTGGCTTCGG CTGGCGGGCC ATCAATCCCA GCATGGCTGC CCCCAGCAGT 
CCCAGTTTGA GCCATCACCA GATGATGGGT CCACCAGGCA CTGGCTTCCA TGGTAGCACT 
GTCTCCAGCC CCCAGAGCAG TGCAGCGACC ACCCCGGGGA GCCCCAGCCT GTGTCGGCAC 
CCAGCAGGGG TCTACCAGGT TTCTGGCCTC CACAACAAAG TGGCCACCAC CCCGGGGAGT 
CCCAGCCTGG GCCGGCACCC TGGGGCTCAC CAAGGCAACC TGGCCTCCGG TCTTCATAGC 
AATGCAATAG CCAGCCCTGG AAGCCCCAGC CTGGGCCGTC ACCTCGGAGG GTCTGGATCT 
GTGGTTCCCG GCAGCCCCTG CTTGGACCGG CATGTGGCCT ATGGTGGCTA TTCTACCCCG 
GAGGATCGGA GACCCACACT GTCCCGGCAG AGCAGTGCCT CTGGCTACCA GGCTCCTTCC 
ACGCCCTCCT TCCCTGTCTC CCCTGCCTAC TACCCTGGCC TGAGCAGCCC TGCCACCTCC 
CCGTCACCAG ACTCCGCAGC CTTCCGGCAA GGGAGCCCAA CACCAGCCTT GCCAGAGAAG 
CGAAGGATGT CAGTGGGAGA CCGGGCAGGC AGCCTCCCCA ACTATGCCAC CATCAATGGG 
AAGGTGTCTT CGCCTGTCGC CAGCGGCATG TCCAGTCCCA GTGGGGGCAG CACCGTCTCC 
TTCTCCCACA CTCTGCCCGA CTTCTCCAAG TACTCCATGC CAGACAACAG CCCGGAGACG 
CGGGCTAAAG TGAAGTTTGT CCAGGACACT TCTAAGTATT GGTACAAGCC TGAGATCTCC 
AGGGAGCAGG CCATCGCGCT CCTCAAGGAC CAGGAGCCGG GGGCCTTCAT CATCCGCGAC 
AGTCACTCCT TCCGAGGCGC GTACGGGCTG GCCATGAAGG TGTCTTCGCC ACCTCCAACC 
ATCATGCAGC AGAATAAAAA AGGAGACATG ACCCATGAGC TGGTCAGGCA TTTTCTGATA 
GAGACTGGCC CCAGAGGAGT CAAGCTCAAG GGCTGCCCCA ATGAGCCAAA CTTCGGATCG 
CTGTCTGCCC TGGTCTACCA GCACTCCATC ATCCCATTGG CCCTGCCTTG CAAGCTGGTC 
ATTCCAAACC GAGACCCCAC AGATGAATCG AAAGATAGCT CCGGCCCTGC CAACTCAACT 
GCAGACCTGC TGAAACAAGG GGCAGCCTGC AATGTGCTCT TCGTCAACTC TGTGGACATG 
GAGTCACTCA CTGGGCCACA GGCCATCTCT AAAGCCACAT CTGAGACGTT GGCTGCAGAC 
CCCACGCCAG CTGCCACCAT CGTTCACTTC AAAGTCTCTG CCCAGGGAAT CACTCTGACT 
GACAACCAGA GAAAGCTCTT TTTCAGACGC CACTACCCTC TCAACACTGT CACCTTCTGT 
GACCTGGATC CACAGGAAAG AAAGTGGATG AAAACAGAGG GTGGTGCCCC TGCTAAGCTC 
TTCGGCTTCG TGGCCCGGAA GCAGGGCAGC ACCACGGACA ACGCCTGCCA CCTCTTTGCT 
GAGCTTGACC CCAACCAGCC GGCCTCTGCC ATCGTCAACT TCGTCTCCAA GGTCATGCTG 
AATGCCGGCC AAAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004817.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860306.1 Homo sapiens tensin 1 (TNS1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OHu89846
Clone ID Related Accession (Same CDS sequence)	XM_017004817.1
Accession Version	XM_017004817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC TTGTGAAGGA GAGGAAAGGG GTACAGGCTC GGGAGAAGCA GCCTGCAGAG 
CCCCCAGCCC CTCTGCGGAG GCGGGCGGCC AGTGATGGAC AGTATGAGAA CCAGTCTCCA 
GAAGCCACAT CCCCTCGTAG CCCTGGGGTT CGCTCCCCTG TCCAGTGTGT CTCCCCGGAG 
CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGGCCCA AAGAGCCCCA TTTGCACAGC 
TACAAGGAGG CCTTCGAGGA GATGGAGGGA ACCTCCCCGA GCAGCCCACC ACCCAGTGGG 
GTGCGGTCCC CCCCGGGTCT GGCCAAGACA CCCCTGTCTG CTCTGGGCCT GAAACCTCAC 
AACCCAGCGG ACATCCTGTT GCACCCCACA GGAGTTACCA GAAGACGGAT CCAGCCAGAT 
TCTCTTTCTC AAGAAATAAT CCCTGTTAAT CTCTTTCTCT CTTTTTCCTT TTTTGCTCTT 
CCTCATCTTC ACCACCTGCC CTTCTTTCCT GCCTCTCCTT CCTCCCACCT CTCTTCTCGT 
CTTCCCTCAG AGGAAGATGA GGGGAAGGTG GTGGTCAGGC TGTCAGAAGA GCCCCGGAGC 
TATGTGGAGT CTGTGGCACG GACAGCGGTG GCTGGACCCC GAGCTCAGGA CTCTGAGCCC 
AAGAGCTTTA GTGCTCCAGC CACCCAGGCC TATGGCCATG AGATACCCCT GAGGAACGGG 
ACCCTGGGTG GCTCCTTTGT CTCCCCCAGC CCCCTCTCCA CCAGCAGCCC CATCCTCAGT 
GCTGACAGCA CTTCAGTGGG GAGTTTCCCG TCGGGAGAGA GCAGTGACCA GGGTCCCCGG 
ACGCCCACCC AGCCTCTGTT GGAGTCTGGC TTCCGCTCAG GCAGCCTGGG ACAGCCCAGC 
CCGTCTGCCC AGAGAAACTA CCAGAGCTCT TCTCCTCTCC CGACTGTGGG CAGTAGCTAC 
AGCAGCCCCG ACTACTCACT TCAGCATTTC AGCTCCTCTC CGGAAAGCCA GGCTCGAGCT 
CAGTTCAGTG TGGCTGGCGT CCACACGGTG CCTGGGAGCC CTCAGGCGCG CCACAGAACA 
GTGGGCACCA ACACTCCCCC TAGTCCTGGC TTCGGCTGGC GGGCCATCAA TCCCAGCATG 
GCTGCCCCCA GCAGTCCCAG TTTGAGCCAT CACCAGATGA TGGGTCCACC AGGCACTGGC 
TTCCATGGTA GCACTGTCTC CAGCCCCCAG AGCAGTGCAG CGACCACCCC GGGGAGCCCC 
AGCCTGTGTC GGCACCCAGC AGGGGTCTAC CAGGTTTCTG GCCTCCACAA CAAAGTGGCC 
ACCACCCCGG GGAGTCCCAG CCTGGGCCGG CACCCTGGGG CTCACCAAGG CAACCTGGCC 
TCCGGTCTTC ATAGCAATGC AATAGCCAGC CCTGGAAGCC CCAGCCTGGG CCGTCACCTC 
GGAGGGTCTG GATCTGTGGT TCCCGGCAGC CCCTGCTTGG ACCGGCATGT GGCCTATGGT 
GGCTATTCTA CCCCGGAGGA TCGGAGACCC ACACTGTCCC GGCAGAGCAG TGCCTCTGGC 
TACCAGGCTC CTTCCACGCC CTCCTTCCCT GTCTCCCCTG CCTACTACCC TGGCCTGAGC 
AGCCCTGCCA CCTCCCCGTC ACCAGACTCC GCAGCCTTCC GGCAAGGGAG CCCAACACCA 
GCCTTGCCAG AGAAGCGAAG GATGTCAGTG GGAGACCGGG CAGGCAGCCT CCCCAACTAT 
GCCACCATCA ATGGGAAGGT GTCTTCGCCT GTCGCCAGCG GCATGTCCAG TCCCAGTGGG 
GGCAGCACCG TCTCCTTCTC CCACACTCTG CCCGACTTCT CCAAGTACTC CATGCCAGAC 
AACAGCCCGG AGACGCGGGC TAAAGTGAAG TTTGTCCAGG ACACTTCTAA GTATTGGTAC 
AAGCCTGAGA TCTCCAGGGA GCAGGCCATC GCGCTCCTCA AGGACCAGGA GCCGGGGGCC 
TTCATCATCC GCGACAGTCA CTCCTTCCGA GGCGCGTACG GGCTGGCCAT GAAGGTGTCT 
TCGCCACCTC CAACCATCAT GCAGCAGAAT AAAAAAGGAG ACATGACCCA TGAGCTGGTC 
AGGCATTTTC TGATAGAGAC TGGCCCCAGA GGAGTCAAGC TCAAGGGCTG CCCCAATGAG 
CCAAACTTCG GATCGCTGTC TGCCCTGGTC TACCAGCACT CCATCATCCC ATTGGCCCTG 
CCTTGCAAGC TGGTCATTCC AAACCGAGAC CCCACAGATG AATCGAAAGA TAGCTCCGGC 
CCTGCCAACT CAACTGCAGA CCTGCTGAAA CAAGGGGCAG CCTGCAATGT GCTCTTCGTC 
AACTCTGTGG ACATGGAGTC ACTCACTGGG CCACAGGCCA TCTCTAAAGC CACATCTGAG 
ACGTTGGCTG CAGACCCCAC GCCAGCTGCC ACCATCGTTC ACTTCAAAGT CTCTGCCCAG 
GGAATCACTC TGACTGACAA CCAGAGAAAG CTCTTTTTCA GACGCCACTA CCCTCTCAAC 
ACTGTCACCT TCTGTGACCT GGATCCACAG GAAAGAAAGT GGATGAAAAC AGAGGGTGGT 
GCCCCTGCTA AGCTCTTCGG CTTCGTGGCC CGGAAGCAGG GCAGCACCAC GGACAACGCC 
TGCCACCTCT TTGCTGAGCT TGACCCCAAC CAGCCGGCCT CTGCCATCGT CAACTTCGTC 
TCCAAGGTCA TGCTGAATGC CGGCCAAAAG AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860306.1
CDS	4102..6837
Translation	MEQLVKERKGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004817.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004817.1

ORF Insert Sequence:

ATGGAGCAGC TTGTGAAGGA GAGGAAAGGG GTACAGGCTC GGGAGAAGCA GCCTGCAGAG 
CCCCCAGCCC CTCTGCGGAG GCGGGCGGCC AGTGATGGAC AGTATGAGAA CCAGTCTCCA 
GAAGCCACAT CCCCTCGTAG CCCTGGGGTT CGCTCCCCTG TCCAGTGTGT CTCCCCGGAG 
CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGGCCCA AAGAGCCCCA TTTGCACAGC 
TACAAGGAGG CCTTCGAGGA GATGGAGGGA ACCTCCCCGA GCAGCCCACC ACCCAGTGGG 
GTGCGGTCCC CCCCGGGTCT GGCCAAGACA CCCCTGTCTG CTCTGGGCCT GAAACCTCAC 
AACCCAGCGG ACATCCTGTT GCACCCCACA GGAGTTACCA GAAGACGGAT CCAGCCAGAT 
TCTCTTTCTC AAGAAATAAT CCCTGTTAAT CTCTTTCTCT CTTTTTCCTT TTTTGCTCTT 
CCTCATCTTC ACCACCTGCC CTTCTTTCCT GCCTCTCCTT CCTCCCACCT CTCTTCTCGT 
CTTCCCTCAG AGGAAGATGA GGGGAAGGTG GTGGTCAGGC TGTCAGAAGA GCCCCGGAGC 
TATGTGGAGT CTGTGGCACG GACAGCGGTG GCTGGACCCC GAGCTCAGGA CTCTGAGCCC 
AAGAGCTTTA GTGCTCCAGC CACCCAGGCC TATGGCCATG AGATACCCCT GAGGAACGGG 
ACCCTGGGTG GCTCCTTTGT CTCCCCCAGC CCCCTCTCCA CCAGCAGCCC CATCCTCAGT 
GCTGACAGCA CTTCAGTGGG GAGTTTCCCG TCGGGAGAGA GCAGTGACCA GGGTCCCCGG 
ACGCCCACCC AGCCTCTGTT GGAGTCTGGC TTCCGCTCAG GCAGCCTGGG ACAGCCCAGC 
CCGTCTGCCC AGAGAAACTA CCAGAGCTCT TCTCCTCTCC CGACTGTGGG CAGTAGCTAC 
AGCAGCCCCG ACTACTCACT TCAGCATTTC AGCTCCTCTC CGGAAAGCCA GGCTCGAGCT 
CAGTTCAGTG TGGCTGGCGT CCACACGGTG CCTGGGAGCC CTCAGGCGCG CCACAGAACA 
GTGGGCACCA ACACTCCCCC TAGTCCTGGC TTCGGCTGGC GGGCCATCAA TCCCAGCATG 
GCTGCCCCCA GCAGTCCCAG TTTGAGCCAT CACCAGATGA TGGGTCCACC AGGCACTGGC 
TTCCATGGTA GCACTGTCTC CAGCCCCCAG AGCAGTGCAG CGACCACCCC GGGGAGCCCC 
AGCCTGTGTC GGCACCCAGC AGGGGTCTAC CAGGTTTCTG GCCTCCACAA CAAAGTGGCC 
ACCACCCCGG GGAGTCCCAG CCTGGGCCGG CACCCTGGGG CTCACCAAGG CAACCTGGCC 
TCCGGTCTTC ATAGCAATGC AATAGCCAGC CCTGGAAGCC CCAGCCTGGG CCGTCACCTC 
GGAGGGTCTG GATCTGTGGT TCCCGGCAGC CCCTGCTTGG ACCGGCATGT GGCCTATGGT 
GGCTATTCTA CCCCGGAGGA TCGGAGACCC ACACTGTCCC GGCAGAGCAG TGCCTCTGGC 
TACCAGGCTC CTTCCACGCC CTCCTTCCCT GTCTCCCCTG CCTACTACCC TGGCCTGAGC 
AGCCCTGCCA CCTCCCCGTC ACCAGACTCC GCAGCCTTCC GGCAAGGGAG CCCAACACCA 
GCCTTGCCAG AGAAGCGAAG GATGTCAGTG GGAGACCGGG CAGGCAGCCT CCCCAACTAT 
GCCACCATCA ATGGGAAGGT GTCTTCGCCT GTCGCCAGCG GCATGTCCAG TCCCAGTGGG 
GGCAGCACCG TCTCCTTCTC CCACACTCTG CCCGACTTCT CCAAGTACTC CATGCCAGAC 
AACAGCCCGG AGACGCGGGC TAAAGTGAAG TTTGTCCAGG ACACTTCTAA GTATTGGTAC 
AAGCCTGAGA TCTCCAGGGA GCAGGCCATC GCGCTCCTCA AGGACCAGGA GCCGGGGGCC 
TTCATCATCC GCGACAGTCA CTCCTTCCGA GGCGCGTACG GGCTGGCCAT GAAGGTGTCT 
TCGCCACCTC CAACCATCAT GCAGCAGAAT AAAAAAGGAG ACATGACCCA TGAGCTGGTC 
AGGCATTTTC TGATAGAGAC TGGCCCCAGA GGAGTCAAGC TCAAGGGCTG CCCCAATGAG 
CCAAACTTCG GATCGCTGTC TGCCCTGGTC TACCAGCACT CCATCATCCC ATTGGCCCTG 
CCTTGCAAGC TGGTCATTCC AAACCGAGAC CCCACAGATG AATCGAAAGA TAGCTCCGGC 
CCTGCCAACT CAACTGCAGA CCTGCTGAAA CAAGGGGCAG CCTGCAATGT GCTCTTCGTC 
AACTCTGTGG ACATGGAGTC ACTCACTGGG CCACAGGCCA TCTCTAAAGC CACATCTGAG 
ACGTTGGCTG CAGACCCCAC GCCAGCTGCC ACCATCGTTC ACTTCAAAGT CTCTGCCCAG 
GGAATCACTC TGACTGACAA CCAGAGAAAG CTCTTTTTCA GACGCCACTA CCCTCTCAAC 
ACTGTCACCT TCTGTGACCT GGATCCACAG GAAAGAAAGT GGATGAAAAC AGAGGGTGGT 
GCCCCTGCTA AGCTCTTCGG CTTCGTGGCC CGGAAGCAGG GCAGCACCAC GGACAACGCC 
TGCCACCTCT TTGCTGAGCT TGACCCCAAC CAGCCGGCCT CTGCCATCGT CAACTTCGTC 
TCCAAGGTCA TGCTGAATGC CGGCCAAAAG AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004815.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860304.1 Homo sapiens tensin 1 (TNS1), transcript variant X22, mRNA.

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CloneID	OHu89844
Clone ID Related Accession (Same CDS sequence)	XM_017004815.1 , XM_017004814.1
Accession Version	XM_017004815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCACA CCCAGCACCT CAGGTCCCGC CCAGCAGGGG GCTCGGCTGT GCCCTCCTCT 
GGACGCCACG TTGTCCCAGC CCAGGTTCAT GTCAATGGTG GGGCGTTAGC ATCTGAGCGG 
GAGACAGACA TCCTGGACGA TGAATTGCCA AACCAGGATG GTCACAGTGC GGGCAGCATG 
GGCACACTCT CTTCTCTGGA CGGGGTCACC AACACCAGTG AGGGGGGCTA CCCAGAGGCC 
CTGTCCCCAC TGACCAACGG TCTGGACAAG TCCTACCCCA TGGAGCCTAT GGTCAATGGA 
GGAGGCTACC CCTACGAGTC TGCCAGCCGG GCGGGGCCTG CCCATGCTGG CCACACGGCC 
CCCATGCGGC CCTCCTACTC TGCACAGGAG GGTTTAGCTG GCTACCAGAG GGAGGGGCCC 
CACCCAGCCT GGCCACAGCC AGTGACCACC TCCCACTATG CCCATGACCC CAGCGGTATG 
TTCCGCTCTC AATCCTTTTC GGAAGCTGAA CCCCAGCTGC CCCCAGCTCC GGTCCGAGGG 
GGAAGCAGCC GGGAGGCTGT GCAAAGGGGA CTGAATTCGT GGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC CTCGCCCACC TCCACGCCAG CAGGAAAGAG CCCACTTGGA GAGTCTTGTA 
GCCAGCAGGC CCAGCCCTCA GCCATTGGCA GAGACCCCCA TCCCCAGTCT CCCTGAGTTC 
CCGCGAGCAG CCTCCCAGCA GGAGATTGAA CAGTCCATCG AAACACTCAA TATGCTGATG 
CTGGACCTGG AGCCAGCCTC CGCTGCTGCC CCACTACACA AGTCCCAGAG TGTCCCCGGG 
GCCTGGCCAG GGGCTTCTCC ACTCTCCTCC CAGCCCCTCT CTGGATCCTC CCGTCAGTCC 
CATCCACTGA CCCAGTCCAG ATCTGGCTAT ATCCCCAGTG GGCATTCGTT GGGAACCCCT 
GAGCCAGCCC CACGGGCCTC TCTGGAGTCT GTCCCTCCTG GCAGGTCTTA CTCACCTTAT 
GACTATCAGC CATGTTTGGC TGGGCCTAAC CAGGATTTCC ATTCAAAGAG CCCAGCCTCT 
TCCTCCTTGC CTGCCTTCCT TCCGACCACC CACAGCCCTC CAGGGCCTCA GCAACCCCCA 
GCCTCTCTCC CTGGCCTCAC TGCTCAGCCT CTGCTCTCAC CAAAGGAAGC GACTTCAGAC 
CCCTCCCGGA CTCCAGAGGA GGAGCCATTG AATTTAGAAG GGCTGGTGGC CCACAGGGTA 
GCAGCGTACA ACGCCAGGCT GCAGGGCATC GGACACAGTG GCAGCTTCCC GCCCCCTCCT 
CTCCACCGGC TCCAATCTTC CTCCCTCTCT GGGGTACAGG CTCGGGAGAA GCAGCCTGCA 
GAGCCCCCAG CCCCTCTGCG GAGGCGGGCG GCCAGTGATG GACAGTATGA GAACCAGTCT 
CCAGAAGCCA CATCCCCTCG TAGCCCTGGG GTTCGCTCCC CTGTCCAGTG TGTCTCCCCG 
GAGCTGGCTC TTACCATCGC TCTCAATCCT GGAGGGCGGC CCAAAGAGCC CCATTTGCAC 
AGCTACAAGG AGGCCTTCGA GGAGATGGAG GGAACCTCCC CGAGCAGCCC ACCACCCAGT 
GGGGTGCGGT CCCCCCCGGG TCTGGCCAAG ACACCCCTGT CTGCTCTGGG CCTGAAACCT 
CACAACCCAG CGGACATCCT GTTGCACCCC ACAGGAGTTA CCAGAAGACG GATCCAGCCA 
GATTCTCTTT CTCAAGAAAT AATCCCTGTT AATCTCTTTC TCTCTTTTTC CTTTTTTGCT 
CTTCCTCATC TTCACCACCT GCCCTTCTTT CCTGCCTCTC CTTCCTCCCA CCTCTCTTCT 
CGTCTTCCCT CAGAGGAAGA TGAGGGGAAG GTGGTGGTCA GGCTGTCAGA AGAGCCCCGG 
AGCTATGTGG AGTCTGTGGC ACGGACAGCG GTGGCTGGAC CCCGAGCTCA GGACTCTGAG 
CCCAAGAGCT TTAGTGCTCC AGCCACCCAG GCCTATGGCC ATGAGATACC CCTGAGGAAC 
GGGACCCTGG GTGGCTCCTT TGTCTCCCCC AGCCCCCTCT CCACCAGCAG CCCCATCCTC 
AGTGCTGACA GCACTTCAGT GGGGAGTTTC CCGTCGGGAG AGAGCAGTGA CCAGGGTCCC 
CGGACGCCCA CCCAGCCTCT GTTGGAGTCT GGCTTCCGCT CAGGCAGCCT GGGACAGCCC 
AGCCCGTCTG CCCAGAGAAA CTACCAGAGC TCTTCTCCTC TCCCGACTGT GGGCAGTAGC 
TACAGCAGCC CCGACTACTC ACTTCAGCAT TTCAGCTCCT CTCCGGAAAG CCAGGCTCGA 
GCTCAGTTCA GTGTGGCTGG CGTCCACACG GTGCCTGGGA GCCCTCAGGC GCGCCACAGA 
ACAGTGGGCA CCAACACTCC CCCTAGTCCT GGCTTCGGCT GGCGGGCCAT CAATCCCAGC 
ATGGCTGCCC CCAGCAGTCC CAGTTTGAGC CATCACCAGA TGATGGGTCC ACCAGGCACT 
GGCTTCCATG GTAGCACTGT CTCCAGCCCC CAGAGCAGTG CAGCGACCAC CCCGGGGAGC 
CCCAGCCTGT GTCGGCACCC AGCAGGGGTC TACCAGGTTT CTGGCCTCCA CAACAAAGTG 
GCCACCACCC CGGGGAGTCC CAGCCTGGGC CGGCACCCTG GGGCTCACCA AGGCAACCTG 
GCCTCCGGTC TTCATAGCAA TGCAATAGCC AGCCCTGGAA GCCCCAGCCT GGGCCGTCAC 
CTCGGAGGGT CTGGATCTGT GGTTCCCGGC AGCCCCTGCT TGGACCGGCA TGTGGCCTAT 
GGTGGCTATT CTACCCCGGA GGATCGGAGA CCCACACTGT CCCGGCAGAG CAGTGCCTCT 
GGCTACCAGG CTCCTTCCAC GCCCTCCTTC CCTGTCTCCC CTGCCTACTA CCCTGGCCTG 
AGCAGCCCTG CCACCTCCCC GTCACCAGAC TCCGCAGCCT TCCGGCAAGG GAGCCCAACA 
CCAGCCTTGC CAGAGAAGCG AAGGATGTCA GTGGGAGACC GGGCAGGCAG CCTCCCCAAC 
TATGCCACCA TCAATGGGAA GGTGTCTTCG CCTGTCGCCA GCGGCATGTC CAGTCCCAGT 
GGGGGCAGCA CCGTCTCCTT CTCCCACACT CTGCCCGACT TCTCCAAGTA CTCCATGCCA 
GACAACAGCC CGGAGACGCG GGCTAAAGTG AAGTTTGTCC AGGACACTTC TAAGTATTGG 
TACAAGCCTG AGATCTCCAG GGAGCAGGCC ATCGCGCTCC TCAAGGACCA GGAGCCGGGG 
GCCTTCATCA TCCGCGACAG TCACTCCTTC CGAGGCGCGT ACGGGCTGGC CATGAAGGTG 
TCTTCGCCAC CTCCAACCAT CATGCAGCAG AATAAAAAAG GAGACATGAC CCATGAGCTG 
GTCAGGCATT TTCTGATAGA GACTGGCCCC AGAGGAGTCA AGCTCAAGGG CTGCCCCAAT 
GAGCCAAACT TCGGATCGCT GTCTGCCCTG GTCTACCAGC ACTCCATCAT CCCATTGGCC 
CTGCCTTGCA AGCTGGTCAT TCCAAACCGA GACCCCACAG ATGAATCGAA AGATAGCTCC 
GGCCCTGCCA ACTCAACTGC AGACCTGCTG AAACAAGGGG CAGCCTGCAA TGTGCTCTTC 
GTCAACTCTG TGGACATGGA GTCACTCACT GGGCCACAGG CCATCTCTAA AGCCACATCT 
GAGACGTTGG CTGCAGACCC CACGCCAGCT GCCACCATCG TTCACTTCAA AGTCTCTGCC 
CAGGGAATCA CTCTGACTGA CAACCAGAGA AAGCTCTTTT TCAGACGCCA CTACCCTCTC 
AACACTGTCA CCTTCTGTGA CCTGGATCCA CAGGAAAGAA AGTGGATGAA AACAGAGGGT 
GGTGCCCCTG CTAAGCTCTT CGGCTTCGTG GCCCGGAAGC AGGGCAGCAC CACGGACAAC 
GCCTGCCACC TCTTTGCTGA GCTTGACCCC AACCAGCCGG CCTCTGCCAT CGTCAACTTC 
GTCTCCAAGG TCATGCTGAA TGCCGGCCAA AAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860304.1
CDS	490..4548
Translation	MYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004815.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004815.1

ORF Insert Sequence:

ATGTACCACA CCCAGCACCT CAGGTCCCGC CCAGCAGGGG GCTCGGCTGT GCCCTCCTCT 
GGACGCCACG TTGTCCCAGC CCAGGTTCAT GTCAATGGTG GGGCGTTAGC ATCTGAGCGG 
GAGACAGACA TCCTGGACGA TGAATTGCCA AACCAGGATG GTCACAGTGC GGGCAGCATG 
GGCACACTCT CTTCTCTGGA CGGGGTCACC AACACCAGTG AGGGGGGCTA CCCAGAGGCC 
CTGTCCCCAC TGACCAACGG TCTGGACAAG TCCTACCCCA TGGAGCCTAT GGTCAATGGA 
GGAGGCTACC CCTACGAGTC TGCCAGCCGG GCGGGGCCTG CCCATGCTGG CCACACGGCC 
CCCATGCGGC CCTCCTACTC TGCACAGGAG GGTTTAGCTG GCTACCAGAG GGAGGGGCCC 
CACCCAGCCT GGCCACAGCC AGTGACCACC TCCCACTATG CCCATGACCC CAGCGGTATG 
TTCCGCTCTC AATCCTTTTC GGAAGCTGAA CCCCAGCTGC CCCCAGCTCC GGTCCGAGGG 
GGAAGCAGCC GGGAGGCTGT GCAAAGGGGA CTGAATTCGT GGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC CTCGCCCACC TCCACGCCAG CAGGAAAGAG CCCACTTGGA GAGTCTTGTA 
GCCAGCAGGC CCAGCCCTCA GCCATTGGCA GAGACCCCCA TCCCCAGTCT CCCTGAGTTC 
CCGCGAGCAG CCTCCCAGCA GGAGATTGAA CAGTCCATCG AAACACTCAA TATGCTGATG 
CTGGACCTGG AGCCAGCCTC CGCTGCTGCC CCACTACACA AGTCCCAGAG TGTCCCCGGG 
GCCTGGCCAG GGGCTTCTCC ACTCTCCTCC CAGCCCCTCT CTGGATCCTC CCGTCAGTCC 
CATCCACTGA CCCAGTCCAG ATCTGGCTAT ATCCCCAGTG GGCATTCGTT GGGAACCCCT 
GAGCCAGCCC CACGGGCCTC TCTGGAGTCT GTCCCTCCTG GCAGGTCTTA CTCACCTTAT 
GACTATCAGC CATGTTTGGC TGGGCCTAAC CAGGATTTCC ATTCAAAGAG CCCAGCCTCT 
TCCTCCTTGC CTGCCTTCCT TCCGACCACC CACAGCCCTC CAGGGCCTCA GCAACCCCCA 
GCCTCTCTCC CTGGCCTCAC TGCTCAGCCT CTGCTCTCAC CAAAGGAAGC GACTTCAGAC 
CCCTCCCGGA CTCCAGAGGA GGAGCCATTG AATTTAGAAG GGCTGGTGGC CCACAGGGTA 
GCAGCGTACA ACGCCAGGCT GCAGGGCATC GGACACAGTG GCAGCTTCCC GCCCCCTCCT 
CTCCACCGGC TCCAATCTTC CTCCCTCTCT GGGGTACAGG CTCGGGAGAA GCAGCCTGCA 
GAGCCCCCAG CCCCTCTGCG GAGGCGGGCG GCCAGTGATG GACAGTATGA GAACCAGTCT 
CCAGAAGCCA CATCCCCTCG TAGCCCTGGG GTTCGCTCCC CTGTCCAGTG TGTCTCCCCG 
GAGCTGGCTC TTACCATCGC TCTCAATCCT GGAGGGCGGC CCAAAGAGCC CCATTTGCAC 
AGCTACAAGG AGGCCTTCGA GGAGATGGAG GGAACCTCCC CGAGCAGCCC ACCACCCAGT 
GGGGTGCGGT CCCCCCCGGG TCTGGCCAAG ACACCCCTGT CTGCTCTGGG CCTGAAACCT 
CACAACCCAG CGGACATCCT GTTGCACCCC ACAGGAGTTA CCAGAAGACG GATCCAGCCA 
GATTCTCTTT CTCAAGAAAT AATCCCTGTT AATCTCTTTC TCTCTTTTTC CTTTTTTGCT 
CTTCCTCATC TTCACCACCT GCCCTTCTTT CCTGCCTCTC CTTCCTCCCA CCTCTCTTCT 
CGTCTTCCCT CAGAGGAAGA TGAGGGGAAG GTGGTGGTCA GGCTGTCAGA AGAGCCCCGG 
AGCTATGTGG AGTCTGTGGC ACGGACAGCG GTGGCTGGAC CCCGAGCTCA GGACTCTGAG 
CCCAAGAGCT TTAGTGCTCC AGCCACCCAG GCCTATGGCC ATGAGATACC CCTGAGGAAC 
GGGACCCTGG GTGGCTCCTT TGTCTCCCCC AGCCCCCTCT CCACCAGCAG CCCCATCCTC 
AGTGCTGACA GCACTTCAGT GGGGAGTTTC CCGTCGGGAG AGAGCAGTGA CCAGGGTCCC 
CGGACGCCCA CCCAGCCTCT GTTGGAGTCT GGCTTCCGCT CAGGCAGCCT GGGACAGCCC 
AGCCCGTCTG CCCAGAGAAA CTACCAGAGC TCTTCTCCTC TCCCGACTGT GGGCAGTAGC 
TACAGCAGCC CCGACTACTC ACTTCAGCAT TTCAGCTCCT CTCCGGAAAG CCAGGCTCGA 
GCTCAGTTCA GTGTGGCTGG CGTCCACACG GTGCCTGGGA GCCCTCAGGC GCGCCACAGA 
ACAGTGGGCA CCAACACTCC CCCTAGTCCT GGCTTCGGCT GGCGGGCCAT CAATCCCAGC 
ATGGCTGCCC CCAGCAGTCC CAGTTTGAGC CATCACCAGA TGATGGGTCC ACCAGGCACT 
GGCTTCCATG GTAGCACTGT CTCCAGCCCC CAGAGCAGTG CAGCGACCAC CCCGGGGAGC 
CCCAGCCTGT GTCGGCACCC AGCAGGGGTC TACCAGGTTT CTGGCCTCCA CAACAAAGTG 
GCCACCACCC CGGGGAGTCC CAGCCTGGGC CGGCACCCTG GGGCTCACCA AGGCAACCTG 
GCCTCCGGTC TTCATAGCAA TGCAATAGCC AGCCCTGGAA GCCCCAGCCT GGGCCGTCAC 
CTCGGAGGGT CTGGATCTGT GGTTCCCGGC AGCCCCTGCT TGGACCGGCA TGTGGCCTAT 
GGTGGCTATT CTACCCCGGA GGATCGGAGA CCCACACTGT CCCGGCAGAG CAGTGCCTCT 
GGCTACCAGG CTCCTTCCAC GCCCTCCTTC CCTGTCTCCC CTGCCTACTA CCCTGGCCTG 
AGCAGCCCTG CCACCTCCCC GTCACCAGAC TCCGCAGCCT TCCGGCAAGG GAGCCCAACA 
CCAGCCTTGC CAGAGAAGCG AAGGATGTCA GTGGGAGACC GGGCAGGCAG CCTCCCCAAC 
TATGCCACCA TCAATGGGAA GGTGTCTTCG CCTGTCGCCA GCGGCATGTC CAGTCCCAGT 
GGGGGCAGCA CCGTCTCCTT CTCCCACACT CTGCCCGACT TCTCCAAGTA CTCCATGCCA 
GACAACAGCC CGGAGACGCG GGCTAAAGTG AAGTTTGTCC AGGACACTTC TAAGTATTGG 
TACAAGCCTG AGATCTCCAG GGAGCAGGCC ATCGCGCTCC TCAAGGACCA GGAGCCGGGG 
GCCTTCATCA TCCGCGACAG TCACTCCTTC CGAGGCGCGT ACGGGCTGGC CATGAAGGTG 
TCTTCGCCAC CTCCAACCAT CATGCAGCAG AATAAAAAAG GAGACATGAC CCATGAGCTG 
GTCAGGCATT TTCTGATAGA GACTGGCCCC AGAGGAGTCA AGCTCAAGGG CTGCCCCAAT 
GAGCCAAACT TCGGATCGCT GTCTGCCCTG GTCTACCAGC ACTCCATCAT CCCATTGGCC 
CTGCCTTGCA AGCTGGTCAT TCCAAACCGA GACCCCACAG ATGAATCGAA AGATAGCTCC 
GGCCCTGCCA ACTCAACTGC AGACCTGCTG AAACAAGGGG CAGCCTGCAA TGTGCTCTTC 
GTCAACTCTG TGGACATGGA GTCACTCACT GGGCCACAGG CCATCTCTAA AGCCACATCT 
GAGACGTTGG CTGCAGACCC CACGCCAGCT GCCACCATCG TTCACTTCAA AGTCTCTGCC 
CAGGGAATCA CTCTGACTGA CAACCAGAGA AAGCTCTTTT TCAGACGCCA CTACCCTCTC 
AACACTGTCA CCTTCTGTGA CCTGGATCCA CAGGAAAGAA AGTGGATGAA AACAGAGGGT 
GGTGCCCCTG CTAAGCTCTT CGGCTTCGTG GCCCGGAAGC AGGGCAGCAC CACGGACAAC 
GCCTGCCACC TCTTTGCTGA GCTTGACCCC AACCAGCCGG CCTCTGCCAT CGTCAACTTC 
GTCTCCAAGG TCATGCTGAA TGCCGGCCAA AAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004814.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860303.1 Homo sapiens tensin 1 (TNS1), transcript variant X21, mRNA.

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CloneID	OHu89844
Clone ID Related Accession (Same CDS sequence)	XM_017004815.1 , XM_017004814.1
Accession Version	XM_017004814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCACA CCCAGCACCT CAGGTCCCGC CCAGCAGGGG GCTCGGCTGT GCCCTCCTCT 
GGACGCCACG TTGTCCCAGC CCAGGTTCAT GTCAATGGTG GGGCGTTAGC ATCTGAGCGG 
GAGACAGACA TCCTGGACGA TGAATTGCCA AACCAGGATG GTCACAGTGC GGGCAGCATG 
GGCACACTCT CTTCTCTGGA CGGGGTCACC AACACCAGTG AGGGGGGCTA CCCAGAGGCC 
CTGTCCCCAC TGACCAACGG TCTGGACAAG TCCTACCCCA TGGAGCCTAT GGTCAATGGA 
GGAGGCTACC CCTACGAGTC TGCCAGCCGG GCGGGGCCTG CCCATGCTGG CCACACGGCC 
CCCATGCGGC CCTCCTACTC TGCACAGGAG GGTTTAGCTG GCTACCAGAG GGAGGGGCCC 
CACCCAGCCT GGCCACAGCC AGTGACCACC TCCCACTATG CCCATGACCC CAGCGGTATG 
TTCCGCTCTC AATCCTTTTC GGAAGCTGAA CCCCAGCTGC CCCCAGCTCC GGTCCGAGGG 
GGAAGCAGCC GGGAGGCTGT GCAAAGGGGA CTGAATTCGT GGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC CTCGCCCACC TCCACGCCAG CAGGAAAGAG CCCACTTGGA GAGTCTTGTA 
GCCAGCAGGC CCAGCCCTCA GCCATTGGCA GAGACCCCCA TCCCCAGTCT CCCTGAGTTC 
CCGCGAGCAG CCTCCCAGCA GGAGATTGAA CAGTCCATCG AAACACTCAA TATGCTGATG 
CTGGACCTGG AGCCAGCCTC CGCTGCTGCC CCACTACACA AGTCCCAGAG TGTCCCCGGG 
GCCTGGCCAG GGGCTTCTCC ACTCTCCTCC CAGCCCCTCT CTGGATCCTC CCGTCAGTCC 
CATCCACTGA CCCAGTCCAG ATCTGGCTAT ATCCCCAGTG GGCATTCGTT GGGAACCCCT 
GAGCCAGCCC CACGGGCCTC TCTGGAGTCT GTCCCTCCTG GCAGGTCTTA CTCACCTTAT 
GACTATCAGC CATGTTTGGC TGGGCCTAAC CAGGATTTCC ATTCAAAGAG CCCAGCCTCT 
TCCTCCTTGC CTGCCTTCCT TCCGACCACC CACAGCCCTC CAGGGCCTCA GCAACCCCCA 
GCCTCTCTCC CTGGCCTCAC TGCTCAGCCT CTGCTCTCAC CAAAGGAAGC GACTTCAGAC 
CCCTCCCGGA CTCCAGAGGA GGAGCCATTG AATTTAGAAG GGCTGGTGGC CCACAGGGTA 
GCAGCGTACA ACGCCAGGCT GCAGGGCATC GGACACAGTG GCAGCTTCCC GCCCCCTCCT 
CTCCACCGGC TCCAATCTTC CTCCCTCTCT GGGGTACAGG CTCGGGAGAA GCAGCCTGCA 
GAGCCCCCAG CCCCTCTGCG GAGGCGGGCG GCCAGTGATG GACAGTATGA GAACCAGTCT 
CCAGAAGCCA CATCCCCTCG TAGCCCTGGG GTTCGCTCCC CTGTCCAGTG TGTCTCCCCG 
GAGCTGGCTC TTACCATCGC TCTCAATCCT GGAGGGCGGC CCAAAGAGCC CCATTTGCAC 
AGCTACAAGG AGGCCTTCGA GGAGATGGAG GGAACCTCCC CGAGCAGCCC ACCACCCAGT 
GGGGTGCGGT CCCCCCCGGG TCTGGCCAAG ACACCCCTGT CTGCTCTGGG CCTGAAACCT 
CACAACCCAG CGGACATCCT GTTGCACCCC ACAGGAGTTA CCAGAAGACG GATCCAGCCA 
GATTCTCTTT CTCAAGAAAT AATCCCTGTT AATCTCTTTC TCTCTTTTTC CTTTTTTGCT 
CTTCCTCATC TTCACCACCT GCCCTTCTTT CCTGCCTCTC CTTCCTCCCA CCTCTCTTCT 
CGTCTTCCCT CAGAGGAAGA TGAGGGGAAG GTGGTGGTCA GGCTGTCAGA AGAGCCCCGG 
AGCTATGTGG AGTCTGTGGC ACGGACAGCG GTGGCTGGAC CCCGAGCTCA GGACTCTGAG 
CCCAAGAGCT TTAGTGCTCC AGCCACCCAG GCCTATGGCC ATGAGATACC CCTGAGGAAC 
GGGACCCTGG GTGGCTCCTT TGTCTCCCCC AGCCCCCTCT CCACCAGCAG CCCCATCCTC 
AGTGCTGACA GCACTTCAGT GGGGAGTTTC CCGTCGGGAG AGAGCAGTGA CCAGGGTCCC 
CGGACGCCCA CCCAGCCTCT GTTGGAGTCT GGCTTCCGCT CAGGCAGCCT GGGACAGCCC 
AGCCCGTCTG CCCAGAGAAA CTACCAGAGC TCTTCTCCTC TCCCGACTGT GGGCAGTAGC 
TACAGCAGCC CCGACTACTC ACTTCAGCAT TTCAGCTCCT CTCCGGAAAG CCAGGCTCGA 
GCTCAGTTCA GTGTGGCTGG CGTCCACACG GTGCCTGGGA GCCCTCAGGC GCGCCACAGA 
ACAGTGGGCA CCAACACTCC CCCTAGTCCT GGCTTCGGCT GGCGGGCCAT CAATCCCAGC 
ATGGCTGCCC CCAGCAGTCC CAGTTTGAGC CATCACCAGA TGATGGGTCC ACCAGGCACT 
GGCTTCCATG GTAGCACTGT CTCCAGCCCC CAGAGCAGTG CAGCGACCAC CCCGGGGAGC 
CCCAGCCTGT GTCGGCACCC AGCAGGGGTC TACCAGGTTT CTGGCCTCCA CAACAAAGTG 
GCCACCACCC CGGGGAGTCC CAGCCTGGGC CGGCACCCTG GGGCTCACCA AGGCAACCTG 
GCCTCCGGTC TTCATAGCAA TGCAATAGCC AGCCCTGGAA GCCCCAGCCT GGGCCGTCAC 
CTCGGAGGGT CTGGATCTGT GGTTCCCGGC AGCCCCTGCT TGGACCGGCA TGTGGCCTAT 
GGTGGCTATT CTACCCCGGA GGATCGGAGA CCCACACTGT CCCGGCAGAG CAGTGCCTCT 
GGCTACCAGG CTCCTTCCAC GCCCTCCTTC CCTGTCTCCC CTGCCTACTA CCCTGGCCTG 
AGCAGCCCTG CCACCTCCCC GTCACCAGAC TCCGCAGCCT TCCGGCAAGG GAGCCCAACA 
CCAGCCTTGC CAGAGAAGCG AAGGATGTCA GTGGGAGACC GGGCAGGCAG CCTCCCCAAC 
TATGCCACCA TCAATGGGAA GGTGTCTTCG CCTGTCGCCA GCGGCATGTC CAGTCCCAGT 
GGGGGCAGCA CCGTCTCCTT CTCCCACACT CTGCCCGACT TCTCCAAGTA CTCCATGCCA 
GACAACAGCC CGGAGACGCG GGCTAAAGTG AAGTTTGTCC AGGACACTTC TAAGTATTGG 
TACAAGCCTG AGATCTCCAG GGAGCAGGCC ATCGCGCTCC TCAAGGACCA GGAGCCGGGG 
GCCTTCATCA TCCGCGACAG TCACTCCTTC CGAGGCGCGT ACGGGCTGGC CATGAAGGTG 
TCTTCGCCAC CTCCAACCAT CATGCAGCAG AATAAAAAAG GAGACATGAC CCATGAGCTG 
GTCAGGCATT TTCTGATAGA GACTGGCCCC AGAGGAGTCA AGCTCAAGGG CTGCCCCAAT 
GAGCCAAACT TCGGATCGCT GTCTGCCCTG GTCTACCAGC ACTCCATCAT CCCATTGGCC 
CTGCCTTGCA AGCTGGTCAT TCCAAACCGA GACCCCACAG ATGAATCGAA AGATAGCTCC 
GGCCCTGCCA ACTCAACTGC AGACCTGCTG AAACAAGGGG CAGCCTGCAA TGTGCTCTTC 
GTCAACTCTG TGGACATGGA GTCACTCACT GGGCCACAGG CCATCTCTAA AGCCACATCT 
GAGACGTTGG CTGCAGACCC CACGCCAGCT GCCACCATCG TTCACTTCAA AGTCTCTGCC 
CAGGGAATCA CTCTGACTGA CAACCAGAGA AAGCTCTTTT TCAGACGCCA CTACCCTCTC 
AACACTGTCA CCTTCTGTGA CCTGGATCCA CAGGAAAGAA AGTGGATGAA AACAGAGGGT 
GGTGCCCCTG CTAAGCTCTT CGGCTTCGTG GCCCGGAAGC AGGGCAGCAC CACGGACAAC 
GCCTGCCACC TCTTTGCTGA GCTTGACCCC AACCAGCCGG CCTCTGCCAT CGTCAACTTC 
GTCTCCAAGG TCATGCTGAA TGCCGGCCAA AAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860303.1
CDS	424..4482
Translation	MYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004814.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004814.1

ORF Insert Sequence:

ATGTACCACA CCCAGCACCT CAGGTCCCGC CCAGCAGGGG GCTCGGCTGT GCCCTCCTCT 
GGACGCCACG TTGTCCCAGC CCAGGTTCAT GTCAATGGTG GGGCGTTAGC ATCTGAGCGG 
GAGACAGACA TCCTGGACGA TGAATTGCCA AACCAGGATG GTCACAGTGC GGGCAGCATG 
GGCACACTCT CTTCTCTGGA CGGGGTCACC AACACCAGTG AGGGGGGCTA CCCAGAGGCC 
CTGTCCCCAC TGACCAACGG TCTGGACAAG TCCTACCCCA TGGAGCCTAT GGTCAATGGA 
GGAGGCTACC CCTACGAGTC TGCCAGCCGG GCGGGGCCTG CCCATGCTGG CCACACGGCC 
CCCATGCGGC CCTCCTACTC TGCACAGGAG GGTTTAGCTG GCTACCAGAG GGAGGGGCCC 
CACCCAGCCT GGCCACAGCC AGTGACCACC TCCCACTATG CCCATGACCC CAGCGGTATG 
TTCCGCTCTC AATCCTTTTC GGAAGCTGAA CCCCAGCTGC CCCCAGCTCC GGTCCGAGGG 
GGAAGCAGCC GGGAGGCTGT GCAAAGGGGA CTGAATTCGT GGCAGCAGCA GCAGCAGCAG 
CAGCAGCAGC CTCGCCCACC TCCACGCCAG CAGGAAAGAG CCCACTTGGA GAGTCTTGTA 
GCCAGCAGGC CCAGCCCTCA GCCATTGGCA GAGACCCCCA TCCCCAGTCT CCCTGAGTTC 
CCGCGAGCAG CCTCCCAGCA GGAGATTGAA CAGTCCATCG AAACACTCAA TATGCTGATG 
CTGGACCTGG AGCCAGCCTC CGCTGCTGCC CCACTACACA AGTCCCAGAG TGTCCCCGGG 
GCCTGGCCAG GGGCTTCTCC ACTCTCCTCC CAGCCCCTCT CTGGATCCTC CCGTCAGTCC 
CATCCACTGA CCCAGTCCAG ATCTGGCTAT ATCCCCAGTG GGCATTCGTT GGGAACCCCT 
GAGCCAGCCC CACGGGCCTC TCTGGAGTCT GTCCCTCCTG GCAGGTCTTA CTCACCTTAT 
GACTATCAGC CATGTTTGGC TGGGCCTAAC CAGGATTTCC ATTCAAAGAG CCCAGCCTCT 
TCCTCCTTGC CTGCCTTCCT TCCGACCACC CACAGCCCTC CAGGGCCTCA GCAACCCCCA 
GCCTCTCTCC CTGGCCTCAC TGCTCAGCCT CTGCTCTCAC CAAAGGAAGC GACTTCAGAC 
CCCTCCCGGA CTCCAGAGGA GGAGCCATTG AATTTAGAAG GGCTGGTGGC CCACAGGGTA 
GCAGCGTACA ACGCCAGGCT GCAGGGCATC GGACACAGTG GCAGCTTCCC GCCCCCTCCT 
CTCCACCGGC TCCAATCTTC CTCCCTCTCT GGGGTACAGG CTCGGGAGAA GCAGCCTGCA 
GAGCCCCCAG CCCCTCTGCG GAGGCGGGCG GCCAGTGATG GACAGTATGA GAACCAGTCT 
CCAGAAGCCA CATCCCCTCG TAGCCCTGGG GTTCGCTCCC CTGTCCAGTG TGTCTCCCCG 
GAGCTGGCTC TTACCATCGC TCTCAATCCT GGAGGGCGGC CCAAAGAGCC CCATTTGCAC 
AGCTACAAGG AGGCCTTCGA GGAGATGGAG GGAACCTCCC CGAGCAGCCC ACCACCCAGT 
GGGGTGCGGT CCCCCCCGGG TCTGGCCAAG ACACCCCTGT CTGCTCTGGG CCTGAAACCT 
CACAACCCAG CGGACATCCT GTTGCACCCC ACAGGAGTTA CCAGAAGACG GATCCAGCCA 
GATTCTCTTT CTCAAGAAAT AATCCCTGTT AATCTCTTTC TCTCTTTTTC CTTTTTTGCT 
CTTCCTCATC TTCACCACCT GCCCTTCTTT CCTGCCTCTC CTTCCTCCCA CCTCTCTTCT 
CGTCTTCCCT CAGAGGAAGA TGAGGGGAAG GTGGTGGTCA GGCTGTCAGA AGAGCCCCGG 
AGCTATGTGG AGTCTGTGGC ACGGACAGCG GTGGCTGGAC CCCGAGCTCA GGACTCTGAG 
CCCAAGAGCT TTAGTGCTCC AGCCACCCAG GCCTATGGCC ATGAGATACC CCTGAGGAAC 
GGGACCCTGG GTGGCTCCTT TGTCTCCCCC AGCCCCCTCT CCACCAGCAG CCCCATCCTC 
AGTGCTGACA GCACTTCAGT GGGGAGTTTC CCGTCGGGAG AGAGCAGTGA CCAGGGTCCC 
CGGACGCCCA CCCAGCCTCT GTTGGAGTCT GGCTTCCGCT CAGGCAGCCT GGGACAGCCC 
AGCCCGTCTG CCCAGAGAAA CTACCAGAGC TCTTCTCCTC TCCCGACTGT GGGCAGTAGC 
TACAGCAGCC CCGACTACTC ACTTCAGCAT TTCAGCTCCT CTCCGGAAAG CCAGGCTCGA 
GCTCAGTTCA GTGTGGCTGG CGTCCACACG GTGCCTGGGA GCCCTCAGGC GCGCCACAGA 
ACAGTGGGCA CCAACACTCC CCCTAGTCCT GGCTTCGGCT GGCGGGCCAT CAATCCCAGC 
ATGGCTGCCC CCAGCAGTCC CAGTTTGAGC CATCACCAGA TGATGGGTCC ACCAGGCACT 
GGCTTCCATG GTAGCACTGT CTCCAGCCCC CAGAGCAGTG CAGCGACCAC CCCGGGGAGC 
CCCAGCCTGT GTCGGCACCC AGCAGGGGTC TACCAGGTTT CTGGCCTCCA CAACAAAGTG 
GCCACCACCC CGGGGAGTCC CAGCCTGGGC CGGCACCCTG GGGCTCACCA AGGCAACCTG 
GCCTCCGGTC TTCATAGCAA TGCAATAGCC AGCCCTGGAA GCCCCAGCCT GGGCCGTCAC 
CTCGGAGGGT CTGGATCTGT GGTTCCCGGC AGCCCCTGCT TGGACCGGCA TGTGGCCTAT 
GGTGGCTATT CTACCCCGGA GGATCGGAGA CCCACACTGT CCCGGCAGAG CAGTGCCTCT 
GGCTACCAGG CTCCTTCCAC GCCCTCCTTC CCTGTCTCCC CTGCCTACTA CCCTGGCCTG 
AGCAGCCCTG CCACCTCCCC GTCACCAGAC TCCGCAGCCT TCCGGCAAGG GAGCCCAACA 
CCAGCCTTGC CAGAGAAGCG AAGGATGTCA GTGGGAGACC GGGCAGGCAG CCTCCCCAAC 
TATGCCACCA TCAATGGGAA GGTGTCTTCG CCTGTCGCCA GCGGCATGTC CAGTCCCAGT 
GGGGGCAGCA CCGTCTCCTT CTCCCACACT CTGCCCGACT TCTCCAAGTA CTCCATGCCA 
GACAACAGCC CGGAGACGCG GGCTAAAGTG AAGTTTGTCC AGGACACTTC TAAGTATTGG 
TACAAGCCTG AGATCTCCAG GGAGCAGGCC ATCGCGCTCC TCAAGGACCA GGAGCCGGGG 
GCCTTCATCA TCCGCGACAG TCACTCCTTC CGAGGCGCGT ACGGGCTGGC CATGAAGGTG 
TCTTCGCCAC CTCCAACCAT CATGCAGCAG AATAAAAAAG GAGACATGAC CCATGAGCTG 
GTCAGGCATT TTCTGATAGA GACTGGCCCC AGAGGAGTCA AGCTCAAGGG CTGCCCCAAT 
GAGCCAAACT TCGGATCGCT GTCTGCCCTG GTCTACCAGC ACTCCATCAT CCCATTGGCC 
CTGCCTTGCA AGCTGGTCAT TCCAAACCGA GACCCCACAG ATGAATCGAA AGATAGCTCC 
GGCCCTGCCA ACTCAACTGC AGACCTGCTG AAACAAGGGG CAGCCTGCAA TGTGCTCTTC 
GTCAACTCTG TGGACATGGA GTCACTCACT GGGCCACAGG CCATCTCTAA AGCCACATCT 
GAGACGTTGG CTGCAGACCC CACGCCAGCT GCCACCATCG TTCACTTCAA AGTCTCTGCC 
CAGGGAATCA CTCTGACTGA CAACCAGAGA AAGCTCTTTT TCAGACGCCA CTACCCTCTC 
AACACTGTCA CCTTCTGTGA CCTGGATCCA CAGGAAAGAA AGTGGATGAA AACAGAGGGT 
GGTGCCCCTG CTAAGCTCTT CGGCTTCGTG GCCCGGAAGC AGGGCAGCAC CACGGACAAC 
GCCTGCCACC TCTTTGCTGA GCTTGACCCC AACCAGCCGG CCTCTGCCAT CGTCAACTTC 
GTCTCCAAGG TCATGCTGAA TGCCGGCCAA AAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453068.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308836.1 Homo sapiens tensin 1 (TNS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu104939
Clone ID Related Accession (Same CDS sequence)	XM_024453068.1
Accession Version	XM_024453068.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGCG TACAACGCCA GGCTGCAGGG CATCGGACAC 
AGTGGCAGCT TCCCGCCCCC TCCTCTCCAC CGGCTCCAAT CTTCCTCCCT CTCTGGGGTA 
CAGGCTCGGG AGAAGCAGCC TGCAGAGCCC CCAGCCCCTC TGCGGAGGCG GGCGGCCAGT 
GATGGACAGT ATGAGAACCA GTCTCCAGAA GCCACATCCC CTCGTAGCCC TGGGGTTCGC 
TCCCCTGTCC AGTGTGTCTC CCCGGAGCTG GCTCTTACCA TCGCTCTCAA TCCTGGAGGG 
CGGCCCAAAG AGCCCCATTT GCACAGCTAC AAGGAGGCCT TCGAGGAGAT GGAGGGAACC 
TCCCCGAGCA GCCCACCACC CAGTGGGGTG CGGTCCCCCC CGGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCTCACAAC CCAGCGGACA TCCTGTTGCA CCCCACAGGA 
GTTACCAGAA GACGGATCCA GCCAGATTCT CTTTCTCAAG AAATAATCCC TGTTAATCTC 
TTTCTCTCTT TTTCCTTTTT TGCTCTTCCT CATCTTCACC ACCTGCCCTT CTTTCCTGCC 
TCTCCTTCCT CCCACCTCTC TTCTCGTCTT CCCTCAGAGG AAGATGAGGG GAAGGTGGTG 
GTCAGGCTGT CAGAAGAGCC CCGGAGCTAT GTGGAGTCTG TGGCACGGAC AGCGGTGGCT 
GGACCCCGAG CTCAGGACTC TGAGCCCAAG AGCTTTAGTG CTCCAGCCAC CCAGGCCTAT 
GGCCATGAGA TACCCCTGAG GAACGGGACC CTGGGTGGCT CCTTTGTCTC CCCCAGCCCC 
CTCTCCACCA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CAGTGGGGAG TTTCCCGTCG 
GGAGAGAGCA GTGACCAGGG TCCCCGGACG CCCACCCAGC CTCTGTTGGA GTCTGGCTTC 
CGCTCAGGCA GCCTGGGACA GCCCAGCCCG TCTGCCCAGA GAAACTACCA GAGCTCTTCT 
CCTCTCCCGA CTGTGGGCAG TAGCTACAGC AGCCCCGACT ACTCACTTCA GCATTTCAGC 
TCCTCTCCGG AAAGCCAGGC TCGAGCTCAG TTCAGTGTGG CTGGCGTCCA CACGGTGCCT 
GGGAGCCCTC AGGCGCGCCA CAGAACAGTG GGCACCAACA CTCCCCCTAG TCCTGGCTTC 
GGCTGGCGGG CCATCAATCC CAGCATGGCT GCCCCCAGCA GTCCCAGTTT GAGCCATCAC 
CAGATGATGG GTCCACCAGG CACTGGCTTC CATGGTAGCA CTGTCTCCAG CCCCCAGAGC 
AGTGCAGCGA CCACCCCGGG GAGCCCCAGC CTGTGTCGGC ACCCAGCAGG GGTCTACCAG 
GTTTCTGGCC TCCACAACAA AGTGGCCACC ACCCCGGGGA GTCCCAGCCT GGGCCGGCAC 
CCTGGGGCTC ACCAAGGCAA CCTGGCCTCC GGTCTTCATA GCAATGCAAT AGCCAGCCCT 
GGAAGCCCCA GCCTGGGCCG TCACCTCGGA GGGTCTGGAT CTGTGGTTCC CGGCAGCCCC 
TGCTTGGACC GGCATGTGGC CTATGGTGGC TATTCTACCC CGGAGGATCG GAGACCCACA 
CTGTCCCGGC AGAGCAGTGC CTCTGGCTAC CAGGCTCCTT CCACGCCCTC CTTCCCTGTC 
TCCCCTGCCT ACTACCCTGG CCTGAGCAGC CCTGCCACCT CCCCGTCACC AGACTCCGCA 
GCCTTCCGGC AAGGGAGCCC AACACCAGCC TTGCCAGAGA AGCGAAGGAT GTCAGTGGGA 
GACCGGGCAG GCAGCCTCCC CAACTATGCC ACCATCAATG GGAAGGTGTC TTCGCCTGTC 
GCCAGCGGCA TGTCCAGTCC CAGTGGGGGC AGCACCGTCT CCTTCTCCCA CACTCTGCCC 
GACTTCTCCA AGTACTCCAT GCCAGACAAC AGCCCGGAGA CGCGGGCTAA AGTGAAGTTT 
GTCCAGGACA CTTCTAAGTA TTGGTACAAG CCTGAGATCT CCAGGGAGCA GGCCATCGCG 
CTCCTCAAGG ACCAGGAGCC GGGGGCCTTC ATCATCCGCG ACAGTCACTC CTTCCGAGGC 
GCGTACGGGC TGGCCATGAA GGTGTCTTCG CCACCTCCAA CCATCATGCA GCAGAATAAA 
AAAGACATGA CCCATGAGCT GGTCAGGCAT TTTCTGATAG AGACTGGCCC CAGAGGAGTC 
AAGCTCAAGG GCTGCCCCAA TGAGCCAAAC TTCGGATCGC TGTCTGCCCT GGTCTACCAG 
CACTCCATCA TCCCATTGGC CCTGCCTTGC AAGCTGGTCA TTCCAAACCG AGACCCCACA 
GATGAATCGA AAGATAGCTC CGGCCCTGCC AACTCAACTG CAGACCTGCT GAAACAAGGG 
GCAGCCTGCA ATGTGCTCTT CGTCAACTCT GTGGACATGG AGTCACTCAC TGGGCCACAG 
GCCATCTCTA AAGCCACATC TGAGACGTTG GCTGCAGACC CCACGCCAGC TGCCACCATC 
GTTCACTTCA AAGTCTCTGC CCAGGGAATC ACTCTGACTG ACAACCAGAG AAAGCTCTTT 
TTCAGACGCC ACTACCCTCT CAACACTGTC ACCTTCTGTG ACCTGGATCC ACAGGAAAGA 
AAGTGGATGA AAACAGAGGG TGGTGCCCCT GCTAAGCTCT TCGGCTTCGT GGCCCGGAAG 
CAGGGCAGCA CCACGGACAA CGCCTGCCAC CTCTTTGCTG AGCTTGACCC CAACCAGCCG 
GCCTCTGCCA TCGTCAACTT CGTCTCCAAG GTCATGCTGA ATGCCGGCCA AAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308836.1
CDS	190..5829
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCIKVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453068.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453068.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGCG TACAACGCCA GGCTGCAGGG CATCGGACAC 
AGTGGCAGCT TCCCGCCCCC TCCTCTCCAC CGGCTCCAAT CTTCCTCCCT CTCTGGGGTA 
CAGGCTCGGG AGAAGCAGCC TGCAGAGCCC CCAGCCCCTC TGCGGAGGCG GGCGGCCAGT 
GATGGACAGT ATGAGAACCA GTCTCCAGAA GCCACATCCC CTCGTAGCCC TGGGGTTCGC 
TCCCCTGTCC AGTGTGTCTC CCCGGAGCTG GCTCTTACCA TCGCTCTCAA TCCTGGAGGG 
CGGCCCAAAG AGCCCCATTT GCACAGCTAC AAGGAGGCCT TCGAGGAGAT GGAGGGAACC 
TCCCCGAGCA GCCCACCACC CAGTGGGGTG CGGTCCCCCC CGGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCTCACAAC CCAGCGGACA TCCTGTTGCA CCCCACAGGA 
GTTACCAGAA GACGGATCCA GCCAGATTCT CTTTCTCAAG AAATAATCCC TGTTAATCTC 
TTTCTCTCTT TTTCCTTTTT TGCTCTTCCT CATCTTCACC ACCTGCCCTT CTTTCCTGCC 
TCTCCTTCCT CCCACCTCTC TTCTCGTCTT CCCTCAGAGG AAGATGAGGG GAAGGTGGTG 
GTCAGGCTGT CAGAAGAGCC CCGGAGCTAT GTGGAGTCTG TGGCACGGAC AGCGGTGGCT 
GGACCCCGAG CTCAGGACTC TGAGCCCAAG AGCTTTAGTG CTCCAGCCAC CCAGGCCTAT 
GGCCATGAGA TACCCCTGAG GAACGGGACC CTGGGTGGCT CCTTTGTCTC CCCCAGCCCC 
CTCTCCACCA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CAGTGGGGAG TTTCCCGTCG 
GGAGAGAGCA GTGACCAGGG TCCCCGGACG CCCACCCAGC CTCTGTTGGA GTCTGGCTTC 
CGCTCAGGCA GCCTGGGACA GCCCAGCCCG TCTGCCCAGA GAAACTACCA GAGCTCTTCT 
CCTCTCCCGA CTGTGGGCAG TAGCTACAGC AGCCCCGACT ACTCACTTCA GCATTTCAGC 
TCCTCTCCGG AAAGCCAGGC TCGAGCTCAG TTCAGTGTGG CTGGCGTCCA CACGGTGCCT 
GGGAGCCCTC AGGCGCGCCA CAGAACAGTG GGCACCAACA CTCCCCCTAG TCCTGGCTTC 
GGCTGGCGGG CCATCAATCC CAGCATGGCT GCCCCCAGCA GTCCCAGTTT GAGCCATCAC 
CAGATGATGG GTCCACCAGG CACTGGCTTC CATGGTAGCA CTGTCTCCAG CCCCCAGAGC 
AGTGCAGCGA CCACCCCGGG GAGCCCCAGC CTGTGTCGGC ACCCAGCAGG GGTCTACCAG 
GTTTCTGGCC TCCACAACAA AGTGGCCACC ACCCCGGGGA GTCCCAGCCT GGGCCGGCAC 
CCTGGGGCTC ACCAAGGCAA CCTGGCCTCC GGTCTTCATA GCAATGCAAT AGCCAGCCCT 
GGAAGCCCCA GCCTGGGCCG TCACCTCGGA GGGTCTGGAT CTGTGGTTCC CGGCAGCCCC 
TGCTTGGACC GGCATGTGGC CTATGGTGGC TATTCTACCC CGGAGGATCG GAGACCCACA 
CTGTCCCGGC AGAGCAGTGC CTCTGGCTAC CAGGCTCCTT CCACGCCCTC CTTCCCTGTC 
TCCCCTGCCT ACTACCCTGG CCTGAGCAGC CCTGCCACCT CCCCGTCACC AGACTCCGCA 
GCCTTCCGGC AAGGGAGCCC AACACCAGCC TTGCCAGAGA AGCGAAGGAT GTCAGTGGGA 
GACCGGGCAG GCAGCCTCCC CAACTATGCC ACCATCAATG GGAAGGTGTC TTCGCCTGTC 
GCCAGCGGCA TGTCCAGTCC CAGTGGGGGC AGCACCGTCT CCTTCTCCCA CACTCTGCCC 
GACTTCTCCA AGTACTCCAT GCCAGACAAC AGCCCGGAGA CGCGGGCTAA AGTGAAGTTT 
GTCCAGGACA CTTCTAAGTA TTGGTACAAG CCTGAGATCT CCAGGGAGCA GGCCATCGCG 
CTCCTCAAGG ACCAGGAGCC GGGGGCCTTC ATCATCCGCG ACAGTCACTC CTTCCGAGGC 
GCGTACGGGC TGGCCATGAA GGTGTCTTCG CCACCTCCAA CCATCATGCA GCAGAATAAA 
AAAGACATGA CCCATGAGCT GGTCAGGCAT TTTCTGATAG AGACTGGCCC CAGAGGAGTC 
AAGCTCAAGG GCTGCCCCAA TGAGCCAAAC TTCGGATCGC TGTCTGCCCT GGTCTACCAG 
CACTCCATCA TCCCATTGGC CCTGCCTTGC AAGCTGGTCA TTCCAAACCG AGACCCCACA 
GATGAATCGA AAGATAGCTC CGGCCCTGCC AACTCAACTG CAGACCTGCT GAAACAAGGG 
GCAGCCTGCA ATGTGCTCTT CGTCAACTCT GTGGACATGG AGTCACTCAC TGGGCCACAG 
GCCATCTCTA AAGCCACATC TGAGACGTTG GCTGCAGACC CCACGCCAGC TGCCACCATC 
GTTCACTTCA AAGTCTCTGC CCAGGGAATC ACTCTGACTG ACAACCAGAG AAAGCTCTTT 
TTCAGACGCC ACTACCCTCT CAACACTGTC ACCTTCTGTG ACCTGGATCC ACAGGAAAGA 
AAGTGGATGA AAACAGAGGG TGGTGCCCCT GCTAAGCTCT TCGGCTTCGT GGCCCGGAAG 
CAGGGCAGCA CCACGGACAA CGCCTGCCAC CTCTTTGCTG AGCTTGACCC CAACCAGCCG 
GCCTCTGCCA TCGTCAACTT CGTCTCCAAG GTCATGCTGA ATGCCGGCCA AAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453069.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308837.1 Homo sapiens tensin 1 (TNS1), transcript variant X2, mRNA.

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CloneID	OHu104940
Clone ID Related Accession (Same CDS sequence)	XM_024453069.1
Accession Version	XM_024453069.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGGG GTACAGGCTC GGGAGAAGCA GCCTGCAGAG 
CCCCCAGCCC CTCTGCGGAG GCGGGCGGCC AGTGATGGAC AGTATGAGAA CCAGTCTCCA 
GAAGCCACAT CCCCTCGTAG CCCTGGGGTT CGCTCCCCTG TCCAGTGTGT CTCCCCGGAG 
CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGGCCCA AAGAGCCCCA TTTGCACAGC 
TACAAGGAGG CCTTCGAGGA GATGGAGGGA ACCTCCCCGA GCAGCCCACC ACCCAGTGGG 
GTGCGGTCCC CCCCGGGTCT GGCCAAGACA CCCCTGTCTG CTCTGGGCCT GAAACCTCAC 
AACCCAGCGG ACATCCTGTT GCACCCCACA GGAGTTACCA GAAGACGGAT CCAGCCAGAT 
TCTCTTTCTC AAGAAATAAT CCCTGTTAAT CTCTTTCTCT CTTTTTCCTT TTTTGCTCTT 
CCTCATCTTC ACCACCTGCC CTTCTTTCCT GCCTCTCCTT CCTCCCACCT CTCTTCTCGT 
CTTCCCTCAG AGGAAGATGA GGGGAAGGTG GTGGTCAGGC TGTCAGAAGA GCCCCGGAGC 
TATGTGGAGT CTGTGGCACG GACAGCGGTG GCTGGACCCC GAGCTCAGGA CTCTGAGCCC 
AAGAGCTTTA GTGCTCCAGC CACCCAGGCC TATGGCCATG AGATACCCCT GAGGAACGGG 
ACCCTGGGTG GCTCCTTTGT CTCCCCCAGC CCCCTCTCCA CCAGCAGCCC CATCCTCAGT 
GCTGACAGCA CTTCAGTGGG GAGTTTCCCG TCGGGAGAGA GCAGTGACCA GGGTCCCCGG 
ACGCCCACCC AGCCTCTGTT GGAGTCTGGC TTCCGCTCAG GCAGCCTGGG ACAGCCCAGC 
CCGTCTGCCC AGAGAAACTA CCAGAGCTCT TCTCCTCTCC CGACTGTGGG CAGTAGCTAC 
AGCAGCCCCG ACTACTCACT TCAGCATTTC AGCTCCTCTC CGGAAAGCCA GGCTCGAGCT 
CAGTTCAGTG TGGCTGGCGT CCACACGGTG CCTGGGAGCC CTCAGGCGCG CCACAGAACA 
GTGGGCACCA ACACTCCCCC TAGTCCTGGC TTCGGCTGGC GGGCCATCAA TCCCAGCATG 
GCTGCCCCCA GCAGTCCCAG TTTGAGCCAT CACCAGATGA TGGGTCCACC AGGCACTGGC 
TTCCATGGTA GCACTGTCTC CAGCCCCCAG AGCAGTGCAG CGACCACCCC GGGGAGCCCC 
AGCCTGTGTC GGCACCCAGC AGGGGTCTAC CAGGTTTCTG GCCTCCACAA CAAAGTGGCC 
ACCACCCCGG GGAGTCCCAG CCTGGGCCGG CACCCTGGGG CTCACCAAGG CAACCTGGCC 
TCCGGTCTTC ATAGCAATGC AATAGCCAGC CCTGGAAGCC CCAGCCTGGG CCGTCACCTC 
GGAGGGTCTG GATCTGTGGT TCCCGGCAGC CCCTGCTTGG ACCGGCATGT GGCCTATGGT 
GGCTATTCTA CCCCGGAGGA TCGGAGACCC ACACTGTCCC GGCAGAGCAG TGCCTCTGGC 
TACCAGGCTC CTTCCACGCC CTCCTTCCCT GTCTCCCCTG CCTACTACCC TGGCCTGAGC 
AGCCCTGCCA CCTCCCCGTC ACCAGACTCC GCAGCCTTCC GGCAAGGGAG CCCAACACCA 
GCCTTGCCAG AGAAGCGAAG GATGTCAGTG GGAGACCGGG CAGGCAGCCT CCCCAACTAT 
GCCACCATCA ATGGGAAGGT GTCTTCGCCT GTCGCCAGCG GCATGTCCAG TCCCAGTGGG 
GGCAGCACCG TCTCCTTCTC CCACACTCTG CCCGACTTCT CCAAGTACTC CATGCCAGAC 
AACAGCCCGG AGACGCGGGC TAAAGTGAAG TTTGTCCAGG ACACTTCTAA GTATTGGTAC 
AAGCCTGAGA TCTCCAGGGA GCAGGCCATC GCGCTCCTCA AGGACCAGGA GCCGGGGGCC 
TTCATCATCC GCGACAGTCA CTCCTTCCGA GGCGCGTACG GGCTGGCCAT GAAGGTGTCT 
TCGCCACCTC CAACCATCAT GCAGCAGAAT AAAAAAGGAG ACATGACCCA TGAGCTGGTC 
AGGCATTTTC TGATAGAGAC TGGCCCCAGA GGAGTCAAGC TCAAGGGCTG CCCCAATGAG 
CCAAACTTCG GATCGCTGTC TGCCCTGGTC TACCAGCACT CCATCATCCC ATTGGCCCTG 
CCTTGCAAGC TGGTCATTCC AAACCGAGAC CCCACAGATG AATCGAAAGA TAGCTCCGGC 
CCTGCCAACT CAACTGCAGA CCTGCTGAAA CAAGGGGCAG CCTGCAATGT GCTCTTCGTC 
AACTCTGTGG ACATGGAGTC ACTCACTGGG CCACAGGCCA TCTCTAAAGC CACATCTGAG 
ACGTTGGCTG CAGACCCCAC GCCAGCTGCC ACCATCGTTC ACTTCAAAGT CTCTGCCCAG 
GGAATCACTC TGACTGACAA CCAGAGAAAG CTCTTTTTCA GACGCCACTA CCCTCTCAAC 
ACTGTCACCT TCTGTGACCT GGATCCACAG GAAAGAAAGT GGATGAAAAC AGAGGGTGGT 
GCCCCTGCTA AGCTCTTCGG CTTCGTGGCC CGGAAGCAGG GCAGCACCAC GGACAACGCC 
TGCCACCTCT TTGCTGAGCT TGACCCCAAC CAGCCGGCCT CTGCCATCGT CAACTTCGTC 
TCCAAGGTCA TGCTGAATGC CGGCCAAAAG AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308837.1
CDS	190..5745
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCIKVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453069.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453069.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGGG GTACAGGCTC GGGAGAAGCA GCCTGCAGAG 
CCCCCAGCCC CTCTGCGGAG GCGGGCGGCC AGTGATGGAC AGTATGAGAA CCAGTCTCCA 
GAAGCCACAT CCCCTCGTAG CCCTGGGGTT CGCTCCCCTG TCCAGTGTGT CTCCCCGGAG 
CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGGCCCA AAGAGCCCCA TTTGCACAGC 
TACAAGGAGG CCTTCGAGGA GATGGAGGGA ACCTCCCCGA GCAGCCCACC ACCCAGTGGG 
GTGCGGTCCC CCCCGGGTCT GGCCAAGACA CCCCTGTCTG CTCTGGGCCT GAAACCTCAC 
AACCCAGCGG ACATCCTGTT GCACCCCACA GGAGTTACCA GAAGACGGAT CCAGCCAGAT 
TCTCTTTCTC AAGAAATAAT CCCTGTTAAT CTCTTTCTCT CTTTTTCCTT TTTTGCTCTT 
CCTCATCTTC ACCACCTGCC CTTCTTTCCT GCCTCTCCTT CCTCCCACCT CTCTTCTCGT 
CTTCCCTCAG AGGAAGATGA GGGGAAGGTG GTGGTCAGGC TGTCAGAAGA GCCCCGGAGC 
TATGTGGAGT CTGTGGCACG GACAGCGGTG GCTGGACCCC GAGCTCAGGA CTCTGAGCCC 
AAGAGCTTTA GTGCTCCAGC CACCCAGGCC TATGGCCATG AGATACCCCT GAGGAACGGG 
ACCCTGGGTG GCTCCTTTGT CTCCCCCAGC CCCCTCTCCA CCAGCAGCCC CATCCTCAGT 
GCTGACAGCA CTTCAGTGGG GAGTTTCCCG TCGGGAGAGA GCAGTGACCA GGGTCCCCGG 
ACGCCCACCC AGCCTCTGTT GGAGTCTGGC TTCCGCTCAG GCAGCCTGGG ACAGCCCAGC 
CCGTCTGCCC AGAGAAACTA CCAGAGCTCT TCTCCTCTCC CGACTGTGGG CAGTAGCTAC 
AGCAGCCCCG ACTACTCACT TCAGCATTTC AGCTCCTCTC CGGAAAGCCA GGCTCGAGCT 
CAGTTCAGTG TGGCTGGCGT CCACACGGTG CCTGGGAGCC CTCAGGCGCG CCACAGAACA 
GTGGGCACCA ACACTCCCCC TAGTCCTGGC TTCGGCTGGC GGGCCATCAA TCCCAGCATG 
GCTGCCCCCA GCAGTCCCAG TTTGAGCCAT CACCAGATGA TGGGTCCACC AGGCACTGGC 
TTCCATGGTA GCACTGTCTC CAGCCCCCAG AGCAGTGCAG CGACCACCCC GGGGAGCCCC 
AGCCTGTGTC GGCACCCAGC AGGGGTCTAC CAGGTTTCTG GCCTCCACAA CAAAGTGGCC 
ACCACCCCGG GGAGTCCCAG CCTGGGCCGG CACCCTGGGG CTCACCAAGG CAACCTGGCC 
TCCGGTCTTC ATAGCAATGC AATAGCCAGC CCTGGAAGCC CCAGCCTGGG CCGTCACCTC 
GGAGGGTCTG GATCTGTGGT TCCCGGCAGC CCCTGCTTGG ACCGGCATGT GGCCTATGGT 
GGCTATTCTA CCCCGGAGGA TCGGAGACCC ACACTGTCCC GGCAGAGCAG TGCCTCTGGC 
TACCAGGCTC CTTCCACGCC CTCCTTCCCT GTCTCCCCTG CCTACTACCC TGGCCTGAGC 
AGCCCTGCCA CCTCCCCGTC ACCAGACTCC GCAGCCTTCC GGCAAGGGAG CCCAACACCA 
GCCTTGCCAG AGAAGCGAAG GATGTCAGTG GGAGACCGGG CAGGCAGCCT CCCCAACTAT 
GCCACCATCA ATGGGAAGGT GTCTTCGCCT GTCGCCAGCG GCATGTCCAG TCCCAGTGGG 
GGCAGCACCG TCTCCTTCTC CCACACTCTG CCCGACTTCT CCAAGTACTC CATGCCAGAC 
AACAGCCCGG AGACGCGGGC TAAAGTGAAG TTTGTCCAGG ACACTTCTAA GTATTGGTAC 
AAGCCTGAGA TCTCCAGGGA GCAGGCCATC GCGCTCCTCA AGGACCAGGA GCCGGGGGCC 
TTCATCATCC GCGACAGTCA CTCCTTCCGA GGCGCGTACG GGCTGGCCAT GAAGGTGTCT 
TCGCCACCTC CAACCATCAT GCAGCAGAAT AAAAAAGGAG ACATGACCCA TGAGCTGGTC 
AGGCATTTTC TGATAGAGAC TGGCCCCAGA GGAGTCAAGC TCAAGGGCTG CCCCAATGAG 
CCAAACTTCG GATCGCTGTC TGCCCTGGTC TACCAGCACT CCATCATCCC ATTGGCCCTG 
CCTTGCAAGC TGGTCATTCC AAACCGAGAC CCCACAGATG AATCGAAAGA TAGCTCCGGC 
CCTGCCAACT CAACTGCAGA CCTGCTGAAA CAAGGGGCAG CCTGCAATGT GCTCTTCGTC 
AACTCTGTGG ACATGGAGTC ACTCACTGGG CCACAGGCCA TCTCTAAAGC CACATCTGAG 
ACGTTGGCTG CAGACCCCAC GCCAGCTGCC ACCATCGTTC ACTTCAAAGT CTCTGCCCAG 
GGAATCACTC TGACTGACAA CCAGAGAAAG CTCTTTTTCA GACGCCACTA CCCTCTCAAC 
ACTGTCACCT TCTGTGACCT GGATCCACAG GAAAGAAAGT GGATGAAAAC AGAGGGTGGT 
GCCCCTGCTA AGCTCTTCGG CTTCGTGGCC CGGAAGCAGG GCAGCACCAC GGACAACGCC 
TGCCACCTCT TTGCTGAGCT TGACCCCAAC CAGCCGGCCT CTGCCATCGT CAACTTCGTC 
TCCAAGGTCA TGCTGAATGC CGGCCAAAAG AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453071.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308839.1 Homo sapiens tensin 1 (TNS1), transcript variant X4, mRNA.

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CloneID	OHu104941
Clone ID Related Accession (Same CDS sequence)	XM_024453071.1
Accession Version	XM_024453071.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGCG TACAACGCCA GGCTGCAGGG CATCGGACAC 
AGTGGCAGCT TCCCGCCCCC TCCTCTCCAC CGGCTCCAAT CTTCCTCCCT CTCTGGGGTA 
CAGGCTCGGG AGAAGCAGCC TGCAGAGCCC CCAGCCCCTC TGCGGAGGCG GGCGGCCAGT 
GATGGACAGT ATGAGAACCA GTCTCCAGAA GCCACATCCC CTCGTAGCCC TGGGGTTCGC 
TCCCCTGTCC AGTGTGTCTC CCCGGAGCTG GCTCTTACCA TCGCTCTCAA TCCTGGAGGG 
CGGCCCAAAG AGCCCCATTT GCACAGCTAC AAGGAGGCCT TCGAGGAGAT GGAGGGAACC 
TCCCCGAGCA GCCCACCACC CAGTGGGGTG CGGTCCCCCC CGGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCTCACAAC CCAGCGGACA TCCTGTTGCA CCCCACAGGA 
GAGGAAGATG AGGGGAAGGT GGTGGTCAGG CTGTCAGAAG AGCCCCGGAG CTATGTGGAG 
TCTGTGGCAC GGACAGCGGT GGCTGGACCC CGAGCTCAGG ACTCTGAGCC CAAGAGCTTT 
AGTGCTCCAG CCACCCAGGC CTATGGCCAT GAGATACCCC TGAGGAACGG GACCCTGGGT 
GGCTCCTTTG TCTCCCCCAG CCCCCTCTCC ACCAGCAGCC CCATCCTCAG TGCTGACAGC 
ACTTCAGTGG GGAGTTTCCC GTCGGGAGAG AGCAGTGACC AGGGTCCCCG GACGCCCACC 
CAGCCTCTGT TGGAGTCTGG CTTCCGCTCA GGCAGCCTGG GACAGCCCAG CCCGTCTGCC 
CAGAGAAACT ACCAGAGCTC TTCTCCTCTC CCGACTGTGG GCAGTAGCTA CAGCAGCCCC 
GACTACTCAC TTCAGCATTT CAGCTCCTCT CCGGAAAGCC AGGCTCGAGC TCAGTTCAGT 
GTGGCTGGCG TCCACACGGT GCCTGGGAGC CCTCAGGCGC GCCACAGAAC AGTGGGCACC 
AACACTCCCC CTAGTCCTGG CTTCGGCTGG CGGGCCATCA ATCCCAGCAT GGCTGCCCCC 
AGCAGTCCCA GTTTGAGCCA TCACCAGATG ATGGGTCCAC CAGGCACTGG CTTCCATGGT 
AGCACTGTCT CCAGCCCCCA GAGCAGTGCA GCGACCACCC CGGGGAGCCC CAGCCTGTGT 
CGGCACCCAG CAGGGGTCTA CCAGGTTTCT GGCCTCCACA ACAAAGTGGC CACCACCCCG 
GGGAGTCCCA GCCTGGGCCG GCACCCTGGG GCTCACCAAG GCAACCTGGC CTCCGGTCTT 
CATAGCAATG CAATAGCCAG CCCTGGAAGC CCCAGCCTGG GCCGTCACCT CGGAGGGTCT 
GGATCTGTGG TTCCCGGCAG CCCCTGCTTG GACCGGCATG TGGCCTATGG TGGCTATTCT 
ACCCCGGAGG ATCGGAGACC CACACTGTCC CGGCAGAGCA GTGCCTCTGG CTACCAGGCT 
CCTTCCACGC CCTCCTTCCC TGTCTCCCCT GCCTACTACC CTGGCCTGAG CAGCCCTGCC 
ACCTCCCCGT CACCAGACTC CGCAGCCTTC CGGCAAGGGA GCCCAACACC AGCCTTGCCA 
GAGAAGCGAA GGATGTCAGT GGGAGACCGG GCAGGCAGCC TCCCCAACTA TGCCACCATC 
AATGGGAAGG TGTCTTCGCC TGTCGCCAGC GGCATGTCCA GTCCCAGTGG GGGCAGCACC 
GTCTCCTTCT CCCACACTCT GCCCGACTTC TCCAAGTACT CCATGCCAGA CAACAGCCCG 
GAGACGCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCTA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGGG AGCAGGCCAT CGCGCTCCTC AAGGACCAGG AGCCGGGGGC CTTCATCATC 
CGCGACAGTC ACTCCTTCCG AGGCGCGTAC GGGCTGGCCA TGAAGGTGTC TTCGCCACCT 
CCAACCATCA TGCAGCAGAA TAAAAAAGGA GACATGACCC ATGAGCTGGT CAGGCATTTT 
CTGATAGAGA CTGGCCCCAG AGGAGTCAAG CTCAAGGGCT GCCCCAATGA GCCAAACTTC 
GGATCGCTGT CTGCCCTGGT CTACCAGCAC TCCATCATCC CATTGGCCCT GCCTTGCAAG 
CTGGTCATTC CAAACCGAGA CCCCACAGAT GAATCGAAAG ATAGCTCCGG CCCTGCCAAC 
TCAACTGCAG ACCTGCTGAA ACAAGGGGCA GCCTGCAATG TGCTCTTCGT CAACTCTGTG 
GACATGGAGT CACTCACTGG GCCACAGGCC ATCTCTAAAG CCACATCTGA GACGTTGGCT 
GCAGACCCCA CGCCAGCTGC CACCATCGTT CACTTCAAAG TCTCTGCCCA GGGAATCACT 
CTGACTGACA ACCAGAGAAA GCTCTTTTTC AGACGCCACT ACCCTCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA GGAAAGAAAG TGGATGAAAA CAGAGGGTGG TGCCCCTGCT 
AAGCTCTTCG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CGGACAACGC CTGCCACCTC 
TTTGCTGAGC TTGACCCCAA CCAGCCGGCC TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAATG CCGGCCAAAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308839.1
CDS	190..5676
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCIKVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453071.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453071.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGCG TACAACGCCA GGCTGCAGGG CATCGGACAC 
AGTGGCAGCT TCCCGCCCCC TCCTCTCCAC CGGCTCCAAT CTTCCTCCCT CTCTGGGGTA 
CAGGCTCGGG AGAAGCAGCC TGCAGAGCCC CCAGCCCCTC TGCGGAGGCG GGCGGCCAGT 
GATGGACAGT ATGAGAACCA GTCTCCAGAA GCCACATCCC CTCGTAGCCC TGGGGTTCGC 
TCCCCTGTCC AGTGTGTCTC CCCGGAGCTG GCTCTTACCA TCGCTCTCAA TCCTGGAGGG 
CGGCCCAAAG AGCCCCATTT GCACAGCTAC AAGGAGGCCT TCGAGGAGAT GGAGGGAACC 
TCCCCGAGCA GCCCACCACC CAGTGGGGTG CGGTCCCCCC CGGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCTCACAAC CCAGCGGACA TCCTGTTGCA CCCCACAGGA 
GAGGAAGATG AGGGGAAGGT GGTGGTCAGG CTGTCAGAAG AGCCCCGGAG CTATGTGGAG 
TCTGTGGCAC GGACAGCGGT GGCTGGACCC CGAGCTCAGG ACTCTGAGCC CAAGAGCTTT 
AGTGCTCCAG CCACCCAGGC CTATGGCCAT GAGATACCCC TGAGGAACGG GACCCTGGGT 
GGCTCCTTTG TCTCCCCCAG CCCCCTCTCC ACCAGCAGCC CCATCCTCAG TGCTGACAGC 
ACTTCAGTGG GGAGTTTCCC GTCGGGAGAG AGCAGTGACC AGGGTCCCCG GACGCCCACC 
CAGCCTCTGT TGGAGTCTGG CTTCCGCTCA GGCAGCCTGG GACAGCCCAG CCCGTCTGCC 
CAGAGAAACT ACCAGAGCTC TTCTCCTCTC CCGACTGTGG GCAGTAGCTA CAGCAGCCCC 
GACTACTCAC TTCAGCATTT CAGCTCCTCT CCGGAAAGCC AGGCTCGAGC TCAGTTCAGT 
GTGGCTGGCG TCCACACGGT GCCTGGGAGC CCTCAGGCGC GCCACAGAAC AGTGGGCACC 
AACACTCCCC CTAGTCCTGG CTTCGGCTGG CGGGCCATCA ATCCCAGCAT GGCTGCCCCC 
AGCAGTCCCA GTTTGAGCCA TCACCAGATG ATGGGTCCAC CAGGCACTGG CTTCCATGGT 
AGCACTGTCT CCAGCCCCCA GAGCAGTGCA GCGACCACCC CGGGGAGCCC CAGCCTGTGT 
CGGCACCCAG CAGGGGTCTA CCAGGTTTCT GGCCTCCACA ACAAAGTGGC CACCACCCCG 
GGGAGTCCCA GCCTGGGCCG GCACCCTGGG GCTCACCAAG GCAACCTGGC CTCCGGTCTT 
CATAGCAATG CAATAGCCAG CCCTGGAAGC CCCAGCCTGG GCCGTCACCT CGGAGGGTCT 
GGATCTGTGG TTCCCGGCAG CCCCTGCTTG GACCGGCATG TGGCCTATGG TGGCTATTCT 
ACCCCGGAGG ATCGGAGACC CACACTGTCC CGGCAGAGCA GTGCCTCTGG CTACCAGGCT 
CCTTCCACGC CCTCCTTCCC TGTCTCCCCT GCCTACTACC CTGGCCTGAG CAGCCCTGCC 
ACCTCCCCGT CACCAGACTC CGCAGCCTTC CGGCAAGGGA GCCCAACACC AGCCTTGCCA 
GAGAAGCGAA GGATGTCAGT GGGAGACCGG GCAGGCAGCC TCCCCAACTA TGCCACCATC 
AATGGGAAGG TGTCTTCGCC TGTCGCCAGC GGCATGTCCA GTCCCAGTGG GGGCAGCACC 
GTCTCCTTCT CCCACACTCT GCCCGACTTC TCCAAGTACT CCATGCCAGA CAACAGCCCG 
GAGACGCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCTA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGGG AGCAGGCCAT CGCGCTCCTC AAGGACCAGG AGCCGGGGGC CTTCATCATC 
CGCGACAGTC ACTCCTTCCG AGGCGCGTAC GGGCTGGCCA TGAAGGTGTC TTCGCCACCT 
CCAACCATCA TGCAGCAGAA TAAAAAAGGA GACATGACCC ATGAGCTGGT CAGGCATTTT 
CTGATAGAGA CTGGCCCCAG AGGAGTCAAG CTCAAGGGCT GCCCCAATGA GCCAAACTTC 
GGATCGCTGT CTGCCCTGGT CTACCAGCAC TCCATCATCC CATTGGCCCT GCCTTGCAAG 
CTGGTCATTC CAAACCGAGA CCCCACAGAT GAATCGAAAG ATAGCTCCGG CCCTGCCAAC 
TCAACTGCAG ACCTGCTGAA ACAAGGGGCA GCCTGCAATG TGCTCTTCGT CAACTCTGTG 
GACATGGAGT CACTCACTGG GCCACAGGCC ATCTCTAAAG CCACATCTGA GACGTTGGCT 
GCAGACCCCA CGCCAGCTGC CACCATCGTT CACTTCAAAG TCTCTGCCCA GGGAATCACT 
CTGACTGACA ACCAGAGAAA GCTCTTTTTC AGACGCCACT ACCCTCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA GGAAAGAAAG TGGATGAAAA CAGAGGGTGG TGCCCCTGCT 
AAGCTCTTCG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CGGACAACGC CTGCCACCTC 
TTTGCTGAGC TTGACCCCAA CCAGCCGGCC TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAATG CCGGCCAAAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453073.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308841.1 Homo sapiens tensin 1 (TNS1), transcript variant X6, mRNA.

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CloneID	OHu104942
Clone ID Related Accession (Same CDS sequence)	XM_024453073.1
Accession Version	XM_024453073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGCG TACAACGCCA GGCTGCAGGG CATCGGACAC 
AGTGGCAGCT TCCCGCCCCC TCCTCTCCAC CGGCTCCAAT CTTCCTCCCT CTCTGGGGTA 
CAGGCTCGGG AGAAGCAGCC TGCAGAGCCC CCAGCCCCTC TGCGGAGGCG GGCGGCCAGT 
GATGGACAGT ATGAGAACCA GTCTCCAGAA GCCACATCCC CTCGTAGCCC TGGGGTTCGC 
TCCCCTGTCC AGTGTGTCTC CCCGGAGCTG GCTCTTACCA TCGCTCTCAA TCCTGGAGGG 
CGGCCCAAAG AGCCCCATTT GCACAGCTAC AAGGAGGCCT TCGAGGAGAT GGAGGGAACC 
TCCCCGAGCA GCCCACCACC CAGTGGGGTG CGGTCCCCCC CGGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCTCACAAC CCAGCGGACA TCCTGTTGCA CCCCACAGGA 
GTTACCAGAA GACGGATCCA GCCAGAGCCC CGGAGCTATG TGGAGTCTGT GGCACGGACA 
GCGGTGGCTG GACCCCGAGC TCAGGACTCT GAGCCCAAGA GCTTTAGTGC TCCAGCCACC 
CAGGCCTATG GCCATGAGAT ACCCCTGAGG AACGGGACCC TGGGTGGCTC CTTTGTCTCC 
CCCAGCCCCC TCTCCACCAG CAGCCCCATC CTCAGTGCTG ACAGCACTTC AGTGGGGAGT 
TTCCCGTCGG GAGAGAGCAG TGACCAGGGT CCCCGGACGC CCACCCAGCC TCTGTTGGAG 
TCTGGCTTCC GCTCAGGCAG CCTGGGACAG CCCAGCCCGT CTGCCCAGAG AAACTACCAG 
AGCTCTTCTC CTCTCCCGAC TGTGGGCAGT AGCTACAGCA GCCCCGACTA CTCACTTCAG 
CATTTCAGCT CCTCTCCGGA AAGCCAGGCT CGAGCTCAGT TCAGTGTGGC TGGCGTCCAC 
ACGGTGCCTG GGAGCCCTCA GGCGCGCCAC AGAACAGTGG GCACCAACAC TCCCCCTAGT 
CCTGGCTTCG GCTGGCGGGC CATCAATCCC AGCATGGCTG CCCCCAGCAG TCCCAGTTTG 
AGCCATCACC AGATGATGGG TCCACCAGGC ACTGGCTTCC ATGGTAGCAC TGTCTCCAGC 
CCCCAGAGCA GTGCAGCGAC CACCCCGGGG AGCCCCAGCC TGTGTCGGCA CCCAGCAGGG 
GTCTACCAGG TTTCTGGCCT CCACAACAAA GTGGCCACCA CCCCGGGGAG TCCCAGCCTG 
GGCCGGCACC CTGGGGCTCA CCAAGGCAAC CTGGCCTCCG GTCTTCATAG CAATGCAATA 
GCCAGCCCTG GAAGCCCCAG CCTGGGCCGT CACCTCGGAG GGTCTGGATC TGTGGTTCCC 
GGCAGCCCCT GCTTGGACCG GCATGTGGCC TATGGTGGCT ATTCTACCCC GGAGGATCGG 
AGACCCACAC TGTCCCGGCA GAGCAGTGCC TCTGGCTACC AGGCTCCTTC CACGCCCTCC 
TTCCCTGTCT CCCCTGCCTA CTACCCTGGC CTGAGCAGCC CTGCCACCTC CCCGTCACCA 
GACTCCGCAG CCTTCCGGCA AGGGAGCCCA ACACCAGCCT TGCCAGAGAA GCGAAGGATG 
TCAGTGGGAG ACCGGGCAGG CAGCCTCCCC AACTATGCCA CCATCAATGG GAAGGTGTCT 
TCGCCTGTCG CCAGCGGCAT GTCCAGTCCC AGTGGGGGCA GCACCGTCTC CTTCTCCCAC 
ACTCTGCCCG ACTTCTCCAA GTACTCCATG CCAGACAACA GCCCGGAGAC GCGGGCTAAA 
GTGAAGTTTG TCCAGGACAC TTCTAAGTAT TGGTACAAGC CTGAGATCTC CAGGGAGCAG 
GCCATCGCGC TCCTCAAGGA CCAGGAGCCG GGGGCCTTCA TCATCCGCGA CAGTCACTCC 
TTCCGAGGCG CGTACGGGCT GGCCATGAAG GTGTCTTCGC CACCTCCAAC CATCATGCAG 
CAGAATAAAA AAGGAGACAT GACCCATGAG CTGGTCAGGC ATTTTCTGAT AGAGACTGGC 
CCCAGAGGAG TCAAGCTCAA GGGCTGCCCC AATGAGCCAA ACTTCGGATC GCTGTCTGCC 
CTGGTCTACC AGCACTCCAT CATCCCATTG GCCCTGCCTT GCAAGCTGGT CATTCCAAAC 
CGAGACCCCA CAGATGAATC GAAAGATAGC TCCGGCCCTG CCAACTCAAC TGCAGACCTG 
CTGAAACAAG GGGCAGCCTG CAATGTGCTC TTCGTCAACT CTGTGGACAT GGAGTCACTC 
ACTGGGCCAC AGGCCATCTC TAAAGCCACA TCTGAGACGT TGGCTGCAGA CCCCACGCCA 
GCTGCCACCA TCGTTCACTT CAAAGTCTCT GCCCAGGGAA TCACTCTGAC TGACAACCAG 
AGAAAGCTCT TTTTCAGACG CCACTACCCT CTCAACACTG TCACCTTCTG TGACCTGGAT 
CCACAGGAAA GAAAGTGGAT GAAAACAGAG GGTGGTGCCC CTGCTAAGCT CTTCGGCTTC 
GTGGCCCGGA AGCAGGGCAG CACCACGGAC AACGCCTGCC ACCTCTTTGC TGAGCTTGAC 
CCCAACCAGC CGGCCTCTGC CATCGTCAAC TTCGTCTCCA AGGTCATGCT GAATGCCGGC 
CAAAAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308841.1
CDS	190..5661
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCIKVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453073.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453073.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGCG TACAACGCCA GGCTGCAGGG CATCGGACAC 
AGTGGCAGCT TCCCGCCCCC TCCTCTCCAC CGGCTCCAAT CTTCCTCCCT CTCTGGGGTA 
CAGGCTCGGG AGAAGCAGCC TGCAGAGCCC CCAGCCCCTC TGCGGAGGCG GGCGGCCAGT 
GATGGACAGT ATGAGAACCA GTCTCCAGAA GCCACATCCC CTCGTAGCCC TGGGGTTCGC 
TCCCCTGTCC AGTGTGTCTC CCCGGAGCTG GCTCTTACCA TCGCTCTCAA TCCTGGAGGG 
CGGCCCAAAG AGCCCCATTT GCACAGCTAC AAGGAGGCCT TCGAGGAGAT GGAGGGAACC 
TCCCCGAGCA GCCCACCACC CAGTGGGGTG CGGTCCCCCC CGGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCTCACAAC CCAGCGGACA TCCTGTTGCA CCCCACAGGA 
GTTACCAGAA GACGGATCCA GCCAGAGCCC CGGAGCTATG TGGAGTCTGT GGCACGGACA 
GCGGTGGCTG GACCCCGAGC TCAGGACTCT GAGCCCAAGA GCTTTAGTGC TCCAGCCACC 
CAGGCCTATG GCCATGAGAT ACCCCTGAGG AACGGGACCC TGGGTGGCTC CTTTGTCTCC 
CCCAGCCCCC TCTCCACCAG CAGCCCCATC CTCAGTGCTG ACAGCACTTC AGTGGGGAGT 
TTCCCGTCGG GAGAGAGCAG TGACCAGGGT CCCCGGACGC CCACCCAGCC TCTGTTGGAG 
TCTGGCTTCC GCTCAGGCAG CCTGGGACAG CCCAGCCCGT CTGCCCAGAG AAACTACCAG 
AGCTCTTCTC CTCTCCCGAC TGTGGGCAGT AGCTACAGCA GCCCCGACTA CTCACTTCAG 
CATTTCAGCT CCTCTCCGGA AAGCCAGGCT CGAGCTCAGT TCAGTGTGGC TGGCGTCCAC 
ACGGTGCCTG GGAGCCCTCA GGCGCGCCAC AGAACAGTGG GCACCAACAC TCCCCCTAGT 
CCTGGCTTCG GCTGGCGGGC CATCAATCCC AGCATGGCTG CCCCCAGCAG TCCCAGTTTG 
AGCCATCACC AGATGATGGG TCCACCAGGC ACTGGCTTCC ATGGTAGCAC TGTCTCCAGC 
CCCCAGAGCA GTGCAGCGAC CACCCCGGGG AGCCCCAGCC TGTGTCGGCA CCCAGCAGGG 
GTCTACCAGG TTTCTGGCCT CCACAACAAA GTGGCCACCA CCCCGGGGAG TCCCAGCCTG 
GGCCGGCACC CTGGGGCTCA CCAAGGCAAC CTGGCCTCCG GTCTTCATAG CAATGCAATA 
GCCAGCCCTG GAAGCCCCAG CCTGGGCCGT CACCTCGGAG GGTCTGGATC TGTGGTTCCC 
GGCAGCCCCT GCTTGGACCG GCATGTGGCC TATGGTGGCT ATTCTACCCC GGAGGATCGG 
AGACCCACAC TGTCCCGGCA GAGCAGTGCC TCTGGCTACC AGGCTCCTTC CACGCCCTCC 
TTCCCTGTCT CCCCTGCCTA CTACCCTGGC CTGAGCAGCC CTGCCACCTC CCCGTCACCA 
GACTCCGCAG CCTTCCGGCA AGGGAGCCCA ACACCAGCCT TGCCAGAGAA GCGAAGGATG 
TCAGTGGGAG ACCGGGCAGG CAGCCTCCCC AACTATGCCA CCATCAATGG GAAGGTGTCT 
TCGCCTGTCG CCAGCGGCAT GTCCAGTCCC AGTGGGGGCA GCACCGTCTC CTTCTCCCAC 
ACTCTGCCCG ACTTCTCCAA GTACTCCATG CCAGACAACA GCCCGGAGAC GCGGGCTAAA 
GTGAAGTTTG TCCAGGACAC TTCTAAGTAT TGGTACAAGC CTGAGATCTC CAGGGAGCAG 
GCCATCGCGC TCCTCAAGGA CCAGGAGCCG GGGGCCTTCA TCATCCGCGA CAGTCACTCC 
TTCCGAGGCG CGTACGGGCT GGCCATGAAG GTGTCTTCGC CACCTCCAAC CATCATGCAG 
CAGAATAAAA AAGGAGACAT GACCCATGAG CTGGTCAGGC ATTTTCTGAT AGAGACTGGC 
CCCAGAGGAG TCAAGCTCAA GGGCTGCCCC AATGAGCCAA ACTTCGGATC GCTGTCTGCC 
CTGGTCTACC AGCACTCCAT CATCCCATTG GCCCTGCCTT GCAAGCTGGT CATTCCAAAC 
CGAGACCCCA CAGATGAATC GAAAGATAGC TCCGGCCCTG CCAACTCAAC TGCAGACCTG 
CTGAAACAAG GGGCAGCCTG CAATGTGCTC TTCGTCAACT CTGTGGACAT GGAGTCACTC 
ACTGGGCCAC AGGCCATCTC TAAAGCCACA TCTGAGACGT TGGCTGCAGA CCCCACGCCA 
GCTGCCACCA TCGTTCACTT CAAAGTCTCT GCCCAGGGAA TCACTCTGAC TGACAACCAG 
AGAAAGCTCT TTTTCAGACG CCACTACCCT CTCAACACTG TCACCTTCTG TGACCTGGAT 
CCACAGGAAA GAAAGTGGAT GAAAACAGAG GGTGGTGCCC CTGCTAAGCT CTTCGGCTTC 
GTGGCCCGGA AGCAGGGCAG CACCACGGAC AACGCCTGCC ACCTCTTTGC TGAGCTTGAC 
CCCAACCAGC CGGCCTCTGC CATCGTCAAC TTCGTCTCCA AGGTCATGCT GAATGCCGGC 
CAAAAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453074.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308842.1 Homo sapiens tensin 1 (TNS1), transcript variant X7, mRNA.

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CloneID	OHu104943
Clone ID Related Accession (Same CDS sequence)	XM_024453074.1
Accession Version	XM_024453074.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGCG TACAACGCCA GGCTGCAGGG CATCGGACAC 
AGTGGCAGCT TCCCGCCCCC TCCTCTCCAC CGGCTCCAAT CTTCCTCCCT CTCTGGGGTA 
CAGGCTCGGG AGAAGCAGCC TGCAGAGCCC CCAGCCCCTC TGCGGAGGCG GGCGGCCAGT 
GATGGACAGT ATGAGAACCA GTCTCCAGAA GCCACATCCC CTCGTAGCCC TGGGGTTCGC 
TCCCCTGTCC AGTGTGTCTC CCCGGAGCTG GCTCTTACCA TCGCTCTCAA TCCTGGAGGG 
CGGCCCAAAG AGCCCCATTT GCACAGCTAC AAGGAGGCCT TCGAGGAGAT GGAGGGAACC 
TCCCCGAGCA GCCCACCACC CAGTGGGGTG CGGTCCCCCC CGGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCTCACAAC CCAGCGGACA TCCTGTTGCA CCCCACAGGA 
GAGCCCCGGA GCTATGTGGA GTCTGTGGCA CGGACAGCGG TGGCTGGACC CCGAGCTCAG 
GACTCTGAGC CCAAGAGCTT TAGTGCTCCA GCCACCCAGG CCTATGGCCA TGAGATACCC 
CTGAGGAACG GGACCCTGGG TGGCTCCTTT GTCTCCCCCA GCCCCCTCTC CACCAGCAGC 
CCCATCCTCA GTGCTGACAG CACTTCAGTG GGGAGTTTCC CGTCGGGAGA GAGCAGTGAC 
CAGGGTCCCC GGACGCCCAC CCAGCCTCTG TTGGAGTCTG GCTTCCGCTC AGGCAGCCTG 
GGACAGCCCA GCCCGTCTGC CCAGAGAAAC TACCAGAGCT CTTCTCCTCT CCCGACTGTG 
GGCAGTAGCT ACAGCAGCCC CGACTACTCA CTTCAGCATT TCAGCTCCTC TCCGGAAAGC 
CAGGCTCGAG CTCAGTTCAG TGTGGCTGGC GTCCACACGG TGCCTGGGAG CCCTCAGGCG 
CGCCACAGAA CAGTGGGCAC CAACACTCCC CCTAGTCCTG GCTTCGGCTG GCGGGCCATC 
AATCCCAGCA TGGCTGCCCC CAGCAGTCCC AGTTTGAGCC ATCACCAGAT GATGGGTCCA 
CCAGGCACTG GCTTCCATGG TAGCACTGTC TCCAGCCCCC AGAGCAGTGC AGCGACCACC 
CCGGGGAGCC CCAGCCTGTG TCGGCACCCA GCAGGGGTCT ACCAGGTTTC TGGCCTCCAC 
AACAAAGTGG CCACCACCCC GGGGAGTCCC AGCCTGGGCC GGCACCCTGG GGCTCACCAA 
GGCAACCTGG CCTCCGGTCT TCATAGCAAT GCAATAGCCA GCCCTGGAAG CCCCAGCCTG 
GGCCGTCACC TCGGAGGGTC TGGATCTGTG GTTCCCGGCA GCCCCTGCTT GGACCGGCAT 
GTGGCCTATG GTGGCTATTC TACCCCGGAG GATCGGAGAC CCACACTGTC CCGGCAGAGC 
AGTGCCTCTG GCTACCAGGC TCCTTCCACG CCCTCCTTCC CTGTCTCCCC TGCCTACTAC 
CCTGGCCTGA GCAGCCCTGC CACCTCCCCG TCACCAGACT CCGCAGCCTT CCGGCAAGGG 
AGCCCAACAC CAGCCTTGCC AGAGAAGCGA AGGATGTCAG TGGGAGACCG GGCAGGCAGC 
CTCCCCAACT ATGCCACCAT CAATGGGAAG GTGTCTTCGC CTGTCGCCAG CGGCATGTCC 
AGTCCCAGTG GGGGCAGCAC CGTCTCCTTC TCCCACACTC TGCCCGACTT CTCCAAGTAC 
TCCATGCCAG ACAACAGCCC GGAGACGCGG GCTAAAGTGA AGTTTGTCCA GGACACTTCT 
AAGTATTGGT ACAAGCCTGA GATCTCCAGG GAGCAGGCCA TCGCGCTCCT CAAGGACCAG 
GAGCCGGGGG CCTTCATCAT CCGCGACAGT CACTCCTTCC GAGGCGCGTA CGGGCTGGCC 
ATGAAGGTGT CTTCGCCACC TCCAACCATC ATGCAGCAGA ATAAAAAAGG AGACATGACC 
CATGAGCTGG TCAGGCATTT TCTGATAGAG ACTGGCCCCA GAGGAGTCAA GCTCAAGGGC 
TGCCCCAATG AGCCAAACTT CGGATCGCTG TCTGCCCTGG TCTACCAGCA CTCCATCATC 
CCATTGGCCC TGCCTTGCAA GCTGGTCATT CCAAACCGAG ACCCCACAGA TGAATCGAAA 
GATAGCTCCG GCCCTGCCAA CTCAACTGCA GACCTGCTGA AACAAGGGGC AGCCTGCAAT 
GTGCTCTTCG TCAACTCTGT GGACATGGAG TCACTCACTG GGCCACAGGC CATCTCTAAA 
GCCACATCTG AGACGTTGGC TGCAGACCCC ACGCCAGCTG CCACCATCGT TCACTTCAAA 
GTCTCTGCCC AGGGAATCAC TCTGACTGAC AACCAGAGAA AGCTCTTTTT CAGACGCCAC 
TACCCTCTCA ACACTGTCAC CTTCTGTGAC CTGGATCCAC AGGAAAGAAA GTGGATGAAA 
ACAGAGGGTG GTGCCCCTGC TAAGCTCTTC GGCTTCGTGG CCCGGAAGCA GGGCAGCACC 
ACGGACAACG CCTGCCACCT CTTTGCTGAG CTTGACCCCA ACCAGCCGGC CTCTGCCATC 
GTCAACTTCG TCTCCAAGGT CATGCTGAAT GCCGGCCAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308842.1
CDS	190..5637
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCIKVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453074.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453074.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGCG TACAACGCCA GGCTGCAGGG CATCGGACAC 
AGTGGCAGCT TCCCGCCCCC TCCTCTCCAC CGGCTCCAAT CTTCCTCCCT CTCTGGGGTA 
CAGGCTCGGG AGAAGCAGCC TGCAGAGCCC CCAGCCCCTC TGCGGAGGCG GGCGGCCAGT 
GATGGACAGT ATGAGAACCA GTCTCCAGAA GCCACATCCC CTCGTAGCCC TGGGGTTCGC 
TCCCCTGTCC AGTGTGTCTC CCCGGAGCTG GCTCTTACCA TCGCTCTCAA TCCTGGAGGG 
CGGCCCAAAG AGCCCCATTT GCACAGCTAC AAGGAGGCCT TCGAGGAGAT GGAGGGAACC 
TCCCCGAGCA GCCCACCACC CAGTGGGGTG CGGTCCCCCC CGGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCTCACAAC CCAGCGGACA TCCTGTTGCA CCCCACAGGA 
GAGCCCCGGA GCTATGTGGA GTCTGTGGCA CGGACAGCGG TGGCTGGACC CCGAGCTCAG 
GACTCTGAGC CCAAGAGCTT TAGTGCTCCA GCCACCCAGG CCTATGGCCA TGAGATACCC 
CTGAGGAACG GGACCCTGGG TGGCTCCTTT GTCTCCCCCA GCCCCCTCTC CACCAGCAGC 
CCCATCCTCA GTGCTGACAG CACTTCAGTG GGGAGTTTCC CGTCGGGAGA GAGCAGTGAC 
CAGGGTCCCC GGACGCCCAC CCAGCCTCTG TTGGAGTCTG GCTTCCGCTC AGGCAGCCTG 
GGACAGCCCA GCCCGTCTGC CCAGAGAAAC TACCAGAGCT CTTCTCCTCT CCCGACTGTG 
GGCAGTAGCT ACAGCAGCCC CGACTACTCA CTTCAGCATT TCAGCTCCTC TCCGGAAAGC 
CAGGCTCGAG CTCAGTTCAG TGTGGCTGGC GTCCACACGG TGCCTGGGAG CCCTCAGGCG 
CGCCACAGAA CAGTGGGCAC CAACACTCCC CCTAGTCCTG GCTTCGGCTG GCGGGCCATC 
AATCCCAGCA TGGCTGCCCC CAGCAGTCCC AGTTTGAGCC ATCACCAGAT GATGGGTCCA 
CCAGGCACTG GCTTCCATGG TAGCACTGTC TCCAGCCCCC AGAGCAGTGC AGCGACCACC 
CCGGGGAGCC CCAGCCTGTG TCGGCACCCA GCAGGGGTCT ACCAGGTTTC TGGCCTCCAC 
AACAAAGTGG CCACCACCCC GGGGAGTCCC AGCCTGGGCC GGCACCCTGG GGCTCACCAA 
GGCAACCTGG CCTCCGGTCT TCATAGCAAT GCAATAGCCA GCCCTGGAAG CCCCAGCCTG 
GGCCGTCACC TCGGAGGGTC TGGATCTGTG GTTCCCGGCA GCCCCTGCTT GGACCGGCAT 
GTGGCCTATG GTGGCTATTC TACCCCGGAG GATCGGAGAC CCACACTGTC CCGGCAGAGC 
AGTGCCTCTG GCTACCAGGC TCCTTCCACG CCCTCCTTCC CTGTCTCCCC TGCCTACTAC 
CCTGGCCTGA GCAGCCCTGC CACCTCCCCG TCACCAGACT CCGCAGCCTT CCGGCAAGGG 
AGCCCAACAC CAGCCTTGCC AGAGAAGCGA AGGATGTCAG TGGGAGACCG GGCAGGCAGC 
CTCCCCAACT ATGCCACCAT CAATGGGAAG GTGTCTTCGC CTGTCGCCAG CGGCATGTCC 
AGTCCCAGTG GGGGCAGCAC CGTCTCCTTC TCCCACACTC TGCCCGACTT CTCCAAGTAC 
TCCATGCCAG ACAACAGCCC GGAGACGCGG GCTAAAGTGA AGTTTGTCCA GGACACTTCT 
AAGTATTGGT ACAAGCCTGA GATCTCCAGG GAGCAGGCCA TCGCGCTCCT CAAGGACCAG 
GAGCCGGGGG CCTTCATCAT CCGCGACAGT CACTCCTTCC GAGGCGCGTA CGGGCTGGCC 
ATGAAGGTGT CTTCGCCACC TCCAACCATC ATGCAGCAGA ATAAAAAAGG AGACATGACC 
CATGAGCTGG TCAGGCATTT TCTGATAGAG ACTGGCCCCA GAGGAGTCAA GCTCAAGGGC 
TGCCCCAATG AGCCAAACTT CGGATCGCTG TCTGCCCTGG TCTACCAGCA CTCCATCATC 
CCATTGGCCC TGCCTTGCAA GCTGGTCATT CCAAACCGAG ACCCCACAGA TGAATCGAAA 
GATAGCTCCG GCCCTGCCAA CTCAACTGCA GACCTGCTGA AACAAGGGGC AGCCTGCAAT 
GTGCTCTTCG TCAACTCTGT GGACATGGAG TCACTCACTG GGCCACAGGC CATCTCTAAA 
GCCACATCTG AGACGTTGGC TGCAGACCCC ACGCCAGCTG CCACCATCGT TCACTTCAAA 
GTCTCTGCCC AGGGAATCAC TCTGACTGAC AACCAGAGAA AGCTCTTTTT CAGACGCCAC 
TACCCTCTCA ACACTGTCAC CTTCTGTGAC CTGGATCCAC AGGAAAGAAA GTGGATGAAA 
ACAGAGGGTG GTGCCCCTGC TAAGCTCTTC GGCTTCGTGG CCCGGAAGCA GGGCAGCACC 
ACGGACAACG CCTGCCACCT CTTTGCTGAG CTTGACCCCA ACCAGCCGGC CTCTGCCATC 
GTCAACTTCG TCTCCAAGGT CATGCTGAAT GCCGGCCAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453075.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308843.1 Homo sapiens tensin 1 (TNS1), transcript variant X8, mRNA.

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CloneID	OHu104944
Clone ID Related Accession (Same CDS sequence)	XM_024453075.1
Accession Version	XM_024453075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGGG GTACAGGCTC GGGAGAAGCA GCCTGCAGAG 
CCCCCAGCCC CTCTGCGGAG GCGGGCGGCC AGTGATGGAC AGTATGAGAA CCAGTCTCCA 
GAAGCCACAT CCCCTCGTAG CCCTGGGGTT CGCTCCCCTG TCCAGTGTGT CTCCCCGGAG 
CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGGCCCA AAGAGCCCCA TTTGCACAGC 
TACAAGGAGG CCTTCGAGGA GATGGAGGGA ACCTCCCCGA GCAGCCCACC ACCCAGTGGG 
GTGCGGTCCC CCCCGGGTCT GGCCAAGACA CCCCTGTCTG CTCTGGGCCT GAAACCTCAC 
AACCCAGCGG ACATCCTGTT GCACCCCACA GGAGAGGAAG ATGAGGGGAA GGTGGTGGTC 
AGGCTGTCAG AAGAGCCCCG GAGCTATGTG GAGTCTGTGG CACGGACAGC GGTGGCTGGA 
CCCCGAGCTC AGGACTCTGA GCCCAAGAGC TTTAGTGCTC CAGCCACCCA GGCCTATGGC 
CATGAGATAC CCCTGAGGAA CGGGACCCTG GGTGGCTCCT TTGTCTCCCC CAGCCCCCTC 
TCCACCAGCA GCCCCATCCT CAGTGCTGAC AGCACTTCAG TGGGGAGTTT CCCGTCGGGA 
GAGAGCAGTG ACCAGGGTCC CCGGACGCCC ACCCAGCCTC TGTTGGAGTC TGGCTTCCGC 
TCAGGCAGCC TGGGACAGCC CAGCCCGTCT GCCCAGAGAA ACTACCAGAG CTCTTCTCCT 
CTCCCGACTG TGGGCAGTAG CTACAGCAGC CCCGACTACT CACTTCAGCA TTTCAGCTCC 
TCTCCGGAAA GCCAGGCTCG AGCTCAGTTC AGTGTGGCTG GCGTCCACAC GGTGCCTGGG 
AGCCCTCAGG CGCGCCACAG AACAGTGGGC ACCAACACTC CCCCTAGTCC TGGCTTCGGC 
TGGCGGGCCA TCAATCCCAG CATGGCTGCC CCCAGCAGTC CCAGTTTGAG CCATCACCAG 
ATGATGGGTC CACCAGGCAC TGGCTTCCAT GGTAGCACTG TCTCCAGCCC CCAGAGCAGT 
GCAGCGACCA CCCCGGGGAG CCCCAGCCTG TGTCGGCACC CAGCAGGGGT CTACCAGGTT 
TCTGGCCTCC ACAACAAAGT GGCCACCACC CCGGGGAGTC CCAGCCTGGG CCGGCACCCT 
GGGGCTCACC AAGGCAACCT GGCCTCCGGT CTTCATAGCA ATGCAATAGC CAGCCCTGGA 
AGCCCCAGCC TGGGCCGTCA CCTCGGAGGG TCTGGATCTG TGGTTCCCGG CAGCCCCTGC 
TTGGACCGGC ATGTGGCCTA TGGTGGCTAT TCTACCCCGG AGGATCGGAG ACCCACACTG 
TCCCGGCAGA GCAGTGCCTC TGGCTACCAG GCTCCTTCCA CGCCCTCCTT CCCTGTCTCC 
CCTGCCTACT ACCCTGGCCT GAGCAGCCCT GCCACCTCCC CGTCACCAGA CTCCGCAGCC 
TTCCGGCAAG GGAGCCCAAC ACCAGCCTTG CCAGAGAAGC GAAGGATGTC AGTGGGAGAC 
CGGGCAGGCA GCCTCCCCAA CTATGCCACC ATCAATGGGA AGGTGTCTTC GCCTGTCGCC 
AGCGGCATGT CCAGTCCCAG TGGGGGCAGC ACCGTCTCCT TCTCCCACAC TCTGCCCGAC 
TTCTCCAAGT ACTCCATGCC AGACAACAGC CCGGAGACGC GGGCTAAAGT GAAGTTTGTC 
CAGGACACTT CTAAGTATTG GTACAAGCCT GAGATCTCCA GGGAGCAGGC CATCGCGCTC 
CTCAAGGACC AGGAGCCGGG GGCCTTCATC ATCCGCGACA GTCACTCCTT CCGAGGCGCG 
TACGGGCTGG CCATGAAGGT GTCTTCGCCA CCTCCAACCA TCATGCAGCA GAATAAAAAA 
GGAGACATGA CCCATGAGCT GGTCAGGCAT TTTCTGATAG AGACTGGCCC CAGAGGAGTC 
AAGCTCAAGG GCTGCCCCAA TGAGCCAAAC TTCGGATCGC TGTCTGCCCT GGTCTACCAG 
CACTCCATCA TCCCATTGGC CCTGCCTTGC AAGCTGGTCA TTCCAAACCG AGACCCCACA 
GATGAATCGA AAGATAGCTC CGGCCCTGCC AACTCAACTG CAGACCTGCT GAAACAAGGG 
GCAGCCTGCA ATGTGCTCTT CGTCAACTCT GTGGACATGG AGTCACTCAC TGGGCCACAG 
GCCATCTCTA AAGCCACATC TGAGACGTTG GCTGCAGACC CCACGCCAGC TGCCACCATC 
GTTCACTTCA AAGTCTCTGC CCAGGGAATC ACTCTGACTG ACAACCAGAG AAAGCTCTTT 
TTCAGACGCC ACTACCCTCT CAACACTGTC ACCTTCTGTG ACCTGGATCC ACAGGAAAGA 
AAGTGGATGA AAACAGAGGG TGGTGCCCCT GCTAAGCTCT TCGGCTTCGT GGCCCGGAAG 
CAGGGCAGCA CCACGGACAA CGCCTGCCAC CTCTTTGCTG AGCTTGACCC CAACCAGCCG 
GCCTCTGCCA TCGTCAACTT CGTCTCCAAG GTCATGCTGA ATGCCGGCCA AAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308843.1
CDS	190..5589
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCIKVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453075.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453075.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGGG GTACAGGCTC GGGAGAAGCA GCCTGCAGAG 
CCCCCAGCCC CTCTGCGGAG GCGGGCGGCC AGTGATGGAC AGTATGAGAA CCAGTCTCCA 
GAAGCCACAT CCCCTCGTAG CCCTGGGGTT CGCTCCCCTG TCCAGTGTGT CTCCCCGGAG 
CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGGCCCA AAGAGCCCCA TTTGCACAGC 
TACAAGGAGG CCTTCGAGGA GATGGAGGGA ACCTCCCCGA GCAGCCCACC ACCCAGTGGG 
GTGCGGTCCC CCCCGGGTCT GGCCAAGACA CCCCTGTCTG CTCTGGGCCT GAAACCTCAC 
AACCCAGCGG ACATCCTGTT GCACCCCACA GGAGAGGAAG ATGAGGGGAA GGTGGTGGTC 
AGGCTGTCAG AAGAGCCCCG GAGCTATGTG GAGTCTGTGG CACGGACAGC GGTGGCTGGA 
CCCCGAGCTC AGGACTCTGA GCCCAAGAGC TTTAGTGCTC CAGCCACCCA GGCCTATGGC 
CATGAGATAC CCCTGAGGAA CGGGACCCTG GGTGGCTCCT TTGTCTCCCC CAGCCCCCTC 
TCCACCAGCA GCCCCATCCT CAGTGCTGAC AGCACTTCAG TGGGGAGTTT CCCGTCGGGA 
GAGAGCAGTG ACCAGGGTCC CCGGACGCCC ACCCAGCCTC TGTTGGAGTC TGGCTTCCGC 
TCAGGCAGCC TGGGACAGCC CAGCCCGTCT GCCCAGAGAA ACTACCAGAG CTCTTCTCCT 
CTCCCGACTG TGGGCAGTAG CTACAGCAGC CCCGACTACT CACTTCAGCA TTTCAGCTCC 
TCTCCGGAAA GCCAGGCTCG AGCTCAGTTC AGTGTGGCTG GCGTCCACAC GGTGCCTGGG 
AGCCCTCAGG CGCGCCACAG AACAGTGGGC ACCAACACTC CCCCTAGTCC TGGCTTCGGC 
TGGCGGGCCA TCAATCCCAG CATGGCTGCC CCCAGCAGTC CCAGTTTGAG CCATCACCAG 
ATGATGGGTC CACCAGGCAC TGGCTTCCAT GGTAGCACTG TCTCCAGCCC CCAGAGCAGT 
GCAGCGACCA CCCCGGGGAG CCCCAGCCTG TGTCGGCACC CAGCAGGGGT CTACCAGGTT 
TCTGGCCTCC ACAACAAAGT GGCCACCACC CCGGGGAGTC CCAGCCTGGG CCGGCACCCT 
GGGGCTCACC AAGGCAACCT GGCCTCCGGT CTTCATAGCA ATGCAATAGC CAGCCCTGGA 
AGCCCCAGCC TGGGCCGTCA CCTCGGAGGG TCTGGATCTG TGGTTCCCGG CAGCCCCTGC 
TTGGACCGGC ATGTGGCCTA TGGTGGCTAT TCTACCCCGG AGGATCGGAG ACCCACACTG 
TCCCGGCAGA GCAGTGCCTC TGGCTACCAG GCTCCTTCCA CGCCCTCCTT CCCTGTCTCC 
CCTGCCTACT ACCCTGGCCT GAGCAGCCCT GCCACCTCCC CGTCACCAGA CTCCGCAGCC 
TTCCGGCAAG GGAGCCCAAC ACCAGCCTTG CCAGAGAAGC GAAGGATGTC AGTGGGAGAC 
CGGGCAGGCA GCCTCCCCAA CTATGCCACC ATCAATGGGA AGGTGTCTTC GCCTGTCGCC 
AGCGGCATGT CCAGTCCCAG TGGGGGCAGC ACCGTCTCCT TCTCCCACAC TCTGCCCGAC 
TTCTCCAAGT ACTCCATGCC AGACAACAGC CCGGAGACGC GGGCTAAAGT GAAGTTTGTC 
CAGGACACTT CTAAGTATTG GTACAAGCCT GAGATCTCCA GGGAGCAGGC CATCGCGCTC 
CTCAAGGACC AGGAGCCGGG GGCCTTCATC ATCCGCGACA GTCACTCCTT CCGAGGCGCG 
TACGGGCTGG CCATGAAGGT GTCTTCGCCA CCTCCAACCA TCATGCAGCA GAATAAAAAA 
GGAGACATGA CCCATGAGCT GGTCAGGCAT TTTCTGATAG AGACTGGCCC CAGAGGAGTC 
AAGCTCAAGG GCTGCCCCAA TGAGCCAAAC TTCGGATCGC TGTCTGCCCT GGTCTACCAG 
CACTCCATCA TCCCATTGGC CCTGCCTTGC AAGCTGGTCA TTCCAAACCG AGACCCCACA 
GATGAATCGA AAGATAGCTC CGGCCCTGCC AACTCAACTG CAGACCTGCT GAAACAAGGG 
GCAGCCTGCA ATGTGCTCTT CGTCAACTCT GTGGACATGG AGTCACTCAC TGGGCCACAG 
GCCATCTCTA AAGCCACATC TGAGACGTTG GCTGCAGACC CCACGCCAGC TGCCACCATC 
GTTCACTTCA AAGTCTCTGC CCAGGGAATC ACTCTGACTG ACAACCAGAG AAAGCTCTTT 
TTCAGACGCC ACTACCCTCT CAACACTGTC ACCTTCTGTG ACCTGGATCC ACAGGAAAGA 
AAGTGGATGA AAACAGAGGG TGGTGCCCCT GCTAAGCTCT TCGGCTTCGT GGCCCGGAAG 
CAGGGCAGCA CCACGGACAA CGCCTGCCAC CTCTTTGCTG AGCTTGACCC CAACCAGCCG 
GCCTCTGCCA TCGTCAACTT CGTCTCCAAG GTCATGCTGA ATGCCGGCCA AAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453076.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308844.1 Homo sapiens tensin 1 (TNS1), transcript variant X9, mRNA.

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CloneID	OHu104945
Clone ID Related Accession (Same CDS sequence)	XM_024453076.1
Accession Version	XM_024453076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGGG GTACAGGCTC GGGAGAAGCA GCCTGCAGAG 
CCCCCAGCCC CTCTGCGGAG GCGGGCGGCC AGTGATGGAC AGTATGAGAA CCAGTCTCCA 
GAAGCCACAT CCCCTCGTAG CCCTGGGGTT CGCTCCCCTG TCCAGTGTGT CTCCCCGGAG 
CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGGCCCA AAGAGCCCCA TTTGCACAGC 
TACAAGGAGG CCTTCGAGGA GATGGAGGGA ACCTCCCCGA GCAGCCCACC ACCCAGTGGG 
GTGCGGTCCC CCCCGGGTCT GGCCAAGACA CCCCTGTCTG CTCTGGGCCT GAAACCTCAC 
AACCCAGCGG ACATCCTGTT GCACCCCACA GGAGAGCCCC GGAGCTATGT GGAGTCTGTG 
GCACGGACAG CGGTGGCTGG ACCCCGAGCT CAGGACTCTG AGCCCAAGAG CTTTAGTGCT 
CCAGCCACCC AGGCCTATGG CCATGAGATA CCCCTGAGGA ACGGGACCCT GGGTGGCTCC 
TTTGTCTCCC CCAGCCCCCT CTCCACCAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCA 
GTGGGGAGTT TCCCGTCGGG AGAGAGCAGT GACCAGGGTC CCCGGACGCC CACCCAGCCT 
CTGTTGGAGT CTGGCTTCCG CTCAGGCAGC CTGGGACAGC CCAGCCCGTC TGCCCAGAGA 
AACTACCAGA GCTCTTCTCC TCTCCCGACT GTGGGCAGTA GCTACAGCAG CCCCGACTAC 
TCACTTCAGC ATTTCAGCTC CTCTCCGGAA AGCCAGGCTC GAGCTCAGTT CAGTGTGGCT 
GGCGTCCACA CGGTGCCTGG GAGCCCTCAG GCGCGCCACA GAACAGTGGG CACCAACACT 
CCCCCTAGTC CTGGCTTCGG CTGGCGGGCC ATCAATCCCA GCATGGCTGC CCCCAGCAGT 
CCCAGTTTGA GCCATCACCA GATGATGGGT CCACCAGGCA CTGGCTTCCA TGGTAGCACT 
GTCTCCAGCC CCCAGAGCAG TGCAGCGACC ACCCCGGGGA GCCCCAGCCT GTGTCGGCAC 
CCAGCAGGGG TCTACCAGGT TTCTGGCCTC CACAACAAAG TGGCCACCAC CCCGGGGAGT 
CCCAGCCTGG GCCGGCACCC TGGGGCTCAC CAAGGCAACC TGGCCTCCGG TCTTCATAGC 
AATGCAATAG CCAGCCCTGG AAGCCCCAGC CTGGGCCGTC ACCTCGGAGG GTCTGGATCT 
GTGGTTCCCG GCAGCCCCTG CTTGGACCGG CATGTGGCCT ATGGTGGCTA TTCTACCCCG 
GAGGATCGGA GACCCACACT GTCCCGGCAG AGCAGTGCCT CTGGCTACCA GGCTCCTTCC 
ACGCCCTCCT TCCCTGTCTC CCCTGCCTAC TACCCTGGCC TGAGCAGCCC TGCCACCTCC 
CCGTCACCAG ACTCCGCAGC CTTCCGGCAA GGGAGCCCAA CACCAGCCTT GCCAGAGAAG 
CGAAGGATGT CAGTGGGAGA CCGGGCAGGC AGCCTCCCCA ACTATGCCAC CATCAATGGG 
AAGGTGTCTT CGCCTGTCGC CAGCGGCATG TCCAGTCCCA GTGGGGGCAG CACCGTCTCC 
TTCTCCCACA CTCTGCCCGA CTTCTCCAAG TACTCCATGC CAGACAACAG CCCGGAGACG 
CGGGCTAAAG TGAAGTTTGT CCAGGACACT TCTAAGTATT GGTACAAGCC TGAGATCTCC 
AGGGAGCAGG CCATCGCGCT CCTCAAGGAC CAGGAGCCGG GGGCCTTCAT CATCCGCGAC 
AGTCACTCCT TCCGAGGCGC GTACGGGCTG GCCATGAAGG TGTCTTCGCC ACCTCCAACC 
ATCATGCAGC AGAATAAAAA AGGAGACATG ACCCATGAGC TGGTCAGGCA TTTTCTGATA 
GAGACTGGCC CCAGAGGAGT CAAGCTCAAG GGCTGCCCCA ATGAGCCAAA CTTCGGATCG 
CTGTCTGCCC TGGTCTACCA GCACTCCATC ATCCCATTGG CCCTGCCTTG CAAGCTGGTC 
ATTCCAAACC GAGACCCCAC AGATGAATCG AAAGATAGCT CCGGCCCTGC CAACTCAACT 
GCAGACCTGC TGAAACAAGG GGCAGCCTGC AATGTGCTCT TCGTCAACTC TGTGGACATG 
GAGTCACTCA CTGGGCCACA GGCCATCTCT AAAGCCACAT CTGAGACGTT GGCTGCAGAC 
CCCACGCCAG CTGCCACCAT CGTTCACTTC AAAGTCTCTG CCCAGGGAAT CACTCTGACT 
GACAACCAGA GAAAGCTCTT TTTCAGACGC CACTACCCTC TCAACACTGT CACCTTCTGT 
GACCTGGATC CACAGGAAAG AAAGTGGATG AAAACAGAGG GTGGTGCCCC TGCTAAGCTC 
TTCGGCTTCG TGGCCCGGAA GCAGGGCAGC ACCACGGACA ACGCCTGCCA CCTCTTTGCT 
GAGCTTGACC CCAACCAGCC GGCCTCTGCC ATCGTCAACT TCGTCTCCAA GGTCATGCTG 
AATGCCGGCC AAAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308844.1
CDS	190..5550
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCIKVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453076.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453076.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGGG GTACAGGCTC GGGAGAAGCA GCCTGCAGAG 
CCCCCAGCCC CTCTGCGGAG GCGGGCGGCC AGTGATGGAC AGTATGAGAA CCAGTCTCCA 
GAAGCCACAT CCCCTCGTAG CCCTGGGGTT CGCTCCCCTG TCCAGTGTGT CTCCCCGGAG 
CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGGCCCA AAGAGCCCCA TTTGCACAGC 
TACAAGGAGG CCTTCGAGGA GATGGAGGGA ACCTCCCCGA GCAGCCCACC ACCCAGTGGG 
GTGCGGTCCC CCCCGGGTCT GGCCAAGACA CCCCTGTCTG CTCTGGGCCT GAAACCTCAC 
AACCCAGCGG ACATCCTGTT GCACCCCACA GGAGAGCCCC GGAGCTATGT GGAGTCTGTG 
GCACGGACAG CGGTGGCTGG ACCCCGAGCT CAGGACTCTG AGCCCAAGAG CTTTAGTGCT 
CCAGCCACCC AGGCCTATGG CCATGAGATA CCCCTGAGGA ACGGGACCCT GGGTGGCTCC 
TTTGTCTCCC CCAGCCCCCT CTCCACCAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCA 
GTGGGGAGTT TCCCGTCGGG AGAGAGCAGT GACCAGGGTC CCCGGACGCC CACCCAGCCT 
CTGTTGGAGT CTGGCTTCCG CTCAGGCAGC CTGGGACAGC CCAGCCCGTC TGCCCAGAGA 
AACTACCAGA GCTCTTCTCC TCTCCCGACT GTGGGCAGTA GCTACAGCAG CCCCGACTAC 
TCACTTCAGC ATTTCAGCTC CTCTCCGGAA AGCCAGGCTC GAGCTCAGTT CAGTGTGGCT 
GGCGTCCACA CGGTGCCTGG GAGCCCTCAG GCGCGCCACA GAACAGTGGG CACCAACACT 
CCCCCTAGTC CTGGCTTCGG CTGGCGGGCC ATCAATCCCA GCATGGCTGC CCCCAGCAGT 
CCCAGTTTGA GCCATCACCA GATGATGGGT CCACCAGGCA CTGGCTTCCA TGGTAGCACT 
GTCTCCAGCC CCCAGAGCAG TGCAGCGACC ACCCCGGGGA GCCCCAGCCT GTGTCGGCAC 
CCAGCAGGGG TCTACCAGGT TTCTGGCCTC CACAACAAAG TGGCCACCAC CCCGGGGAGT 
CCCAGCCTGG GCCGGCACCC TGGGGCTCAC CAAGGCAACC TGGCCTCCGG TCTTCATAGC 
AATGCAATAG CCAGCCCTGG AAGCCCCAGC CTGGGCCGTC ACCTCGGAGG GTCTGGATCT 
GTGGTTCCCG GCAGCCCCTG CTTGGACCGG CATGTGGCCT ATGGTGGCTA TTCTACCCCG 
GAGGATCGGA GACCCACACT GTCCCGGCAG AGCAGTGCCT CTGGCTACCA GGCTCCTTCC 
ACGCCCTCCT TCCCTGTCTC CCCTGCCTAC TACCCTGGCC TGAGCAGCCC TGCCACCTCC 
CCGTCACCAG ACTCCGCAGC CTTCCGGCAA GGGAGCCCAA CACCAGCCTT GCCAGAGAAG 
CGAAGGATGT CAGTGGGAGA CCGGGCAGGC AGCCTCCCCA ACTATGCCAC CATCAATGGG 
AAGGTGTCTT CGCCTGTCGC CAGCGGCATG TCCAGTCCCA GTGGGGGCAG CACCGTCTCC 
TTCTCCCACA CTCTGCCCGA CTTCTCCAAG TACTCCATGC CAGACAACAG CCCGGAGACG 
CGGGCTAAAG TGAAGTTTGT CCAGGACACT TCTAAGTATT GGTACAAGCC TGAGATCTCC 
AGGGAGCAGG CCATCGCGCT CCTCAAGGAC CAGGAGCCGG GGGCCTTCAT CATCCGCGAC 
AGTCACTCCT TCCGAGGCGC GTACGGGCTG GCCATGAAGG TGTCTTCGCC ACCTCCAACC 
ATCATGCAGC AGAATAAAAA AGGAGACATG ACCCATGAGC TGGTCAGGCA TTTTCTGATA 
GAGACTGGCC CCAGAGGAGT CAAGCTCAAG GGCTGCCCCA ATGAGCCAAA CTTCGGATCG 
CTGTCTGCCC TGGTCTACCA GCACTCCATC ATCCCATTGG CCCTGCCTTG CAAGCTGGTC 
ATTCCAAACC GAGACCCCAC AGATGAATCG AAAGATAGCT CCGGCCCTGC CAACTCAACT 
GCAGACCTGC TGAAACAAGG GGCAGCCTGC AATGTGCTCT TCGTCAACTC TGTGGACATG 
GAGTCACTCA CTGGGCCACA GGCCATCTCT AAAGCCACAT CTGAGACGTT GGCTGCAGAC 
CCCACGCCAG CTGCCACCAT CGTTCACTTC AAAGTCTCTG CCCAGGGAAT CACTCTGACT 
GACAACCAGA GAAAGCTCTT TTTCAGACGC CACTACCCTC TCAACACTGT CACCTTCTGT 
GACCTGGATC CACAGGAAAG AAAGTGGATG AAAACAGAGG GTGGTGCCCC TGCTAAGCTC 
TTCGGCTTCG TGGCCCGGAA GCAGGGCAGC ACCACGGACA ACGCCTGCCA CCTCTTTGCT 
GAGCTTGACC CCAACCAGCC GGCCTCTGCC ATCGTCAACT TCGTCTCCAA GGTCATGCTG 
AATGCCGGCC AAAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453080.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308848.1 Homo sapiens tensin 1 (TNS1), transcript variant X13, mRNA.

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CloneID	OHu104947
Clone ID Related Accession (Same CDS sequence)	XM_024453080.1
Accession Version	XM_024453080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTGG CTGCCCCCTG CGTTCCTCCA TCCAACCATG AGCTGGTGCC CATCACCACT 
GAGAATGCAC CAAAGAATGT AGTGGACAAG GGAGAAGGAG CCTCCCGGGG TGGAAACACA 
CGGAAAAGCC TCGAGGACAA CGGCTCCACC AGGGTCACCC CGAGTGTCCA GCCCCACCTC 
CAGCCCATCA GAAACATGAG TGTGAGCCGG ACCATGGAGG ACAGCTGTGA GCTGGACCTG 
GTGTACGTCA CAGAGAGGAT CATCGCTGTC TCCTTCCCCA GCACAGCCAA TGAGGAGAAC 
TTCCGGAGCA ACCTCCGTGA GGTGGCGCAG ATGCTCAAGT CCAAACATGG AGGCAACTAC 
CTGCTGTTCA ACCTCTCTGA GCGGAGACCT GACATCACGA AGCTCCATGC CAAGGTACTG 
GAATTTGGCT GGCCCGACCT CCACACCCCA GCCCTGGAGA AGATCTGCAG CATCTGTAAG 
GCCATGGACA CATGGCTCAA TGCAGACCCT CACAATGTCG TTGTTCTACA CAACAAGGGA 
AACCGAGGCA GGATAGGAGT TGTCATCGCG GCTTACATGC ACTACAGCAA CATTTCTGCC 
AGTGCGGACC AGGCTCTGGA CCGGTTTGCA ATGAAGCGGT TCTATGAGGA TAAGATTGTG 
CCCATTGGCC AGCCATCCCA AAGAAGGTAC GTGCATTACT TCAGTGGCCT GCTCTCCGGC 
TCCATCAAAA TGAACAACAA GCCCTTGTTT CTGCACCACG TGATCATGCA CGGCATCCCC 
AACTTTGAGT CTAAAGGAGG ATGTCGGCCA TTTCTCCGCA TCTACCAGGC CATGCAACCT 
GTGTACACAT CTGGCATCTA CAACATCCCA GGAGACAGCC AGACTAGCGT CTGCATCACC 
ATCGAGCCAG GACTGCTCTT GAAGGGAGAC ATCTTGCTGA AGTGCTACCA CAAGAAGTTC 
CGAAGCCCAG CCCGAGACGT CATCTTCCGT GTGCAGTTCC ACACCTGTGC CATCCATGAC 
CTGGGGGTTG TCTTTGGGAA GGAGGACCTT GATGATGCTT TCAAAGATGA TCGATTTCCA 
GAGTATGGCA AAGTGGAGTT TGTATTTTCT TATGGGCCAG AGAAAATTCA AGGCATGGAG 
CACCTGGAGA ACGGGCCGAG CGTGTCTGTG GACTATAACA CCTCCGACCC CCTCATCCGC 
TGGGACTCCT ACGACAACTT CAGTGGGCAT CGAGATGACG GCATGGAGGG GGCAGTGAGA 
TCAGATTTTG ATATGAAAGC TCAAAGCTTC TCAGCTCTCT CCTTCTTCCC TGCCTTCCTC 
TCTCTGTCTG CACATTGCTT GAGAACCTTC GAGAAGCAGA TGGGCGCTCC TCATGTGATG 
CAAAGGCAGA AAGGCCCAAG GGTGGTGCCC TGGCTTCTGT CTGCCATGCA GGCCTGTGCT 
TCATGGTTTG GTGGCCTCTG CATAAAGGTG GTGGGACACA CGCAGGGGCC ACTAGATGGG 
AGCCTGTATG CTAAGGTGAA GAAGAAAGAC TCCCTGCACG GCAGCACCGG GGCTGTTAAT 
GCCACACGTC CTACACTGTC GGCCACCCCC AACCACGTGG AACACACGCT TTCTGTGAGC 
AGCGACTCGG GCAACTCCAC AGCCTCCACC AAGACCGACA AGACCGACGA GCCTGTCCCC 
GGGGCCTCCA GTGCCACTGC TGCCTTGAGT CCCCAGGAGA AGCGGGAGCT GGACCGCCTG 
CTGAGTGGCT TTGGCTTAGA GCGAGAGAAG CAAGGCGCCA TGTACCACAC CCAGCACCTC 
AGGTCCCGCC CAGCAGGGGG CTCGGCTGTG CCCTCCTCTG GACGCCACGT TGTCCCAGCC 
CAGGTTCATG TCAATGGTGG GGCGTTAGCA TCTGAGCGGG AGACAGACAT CCTGGACGAT 
GAATTGCCAA ACCAGGATGG TCACAGTGCG GGCAGCATGG GCACACTCTC TTCTCTGGAC 
GGGGTCACCA ACACCAGTGA GGGGGGCTAC CCAGAGGCCC TGTCCCCACT GACCAACGGT 
CTGGACAAGT CCTACCCCAT GGAGCCTATG GTCAATGGAG GAGGCTACCC CTACGAGTCT 
GCCAGCCGGG CGGGGCCTGC CCATGCTGGC CACACGGCCC CCATGCGGCC CTCCTACTCT 
GCACAGGAGG GTTTAGCTGG CTACCAGAGG GAGGGGCCCC ACCCAGCCTG GCCACAGCCA 
GTGACCACCT CCCACTATGC CCATGACCCC AGCGGTATGT TCCGCTCTCA ATCCTTTTCG 
GAAGCTGAAC CCCAGCTGCC CCCAGCTCCG GTCCGAGGGG GAAGCAGCCG GGAGGCTGTG 
CAAAGGGGAC TGAATTCGTG GCAGCAGCAG CAGCAGCAGC AGCAGCAGCC TCGCCCACCT 
CCACGCCAGC AGGAAAGAGC CCACTTGGAG AGTCTTGTAG CCAGCAGGCC CAGCCCTCAG 
CCATTGGCAG AGACCCCCAT CCCCAGTCTC CCTGAGTTCC CGCGAGCAGC CTCCCAGCAG 
GAGATTGAAC AGTCCATCGA AACACTCAAT ATGCTGATGC TGGACCTGGA GCCAGCCTCC 
GCTGCTGCCC CACTACACAA GTCCCAGAGT GTCCCCGGGG CCTGGCCAGG GGCTTCTCCA 
CTCTCCTCCC AGCCCCTCTC TGGATCCTCC CGTCAGTCCC ATCCACTGAC CCAGTCCAGA 
TCTGGCTATA TCCCCAGTGG GCATTCGTTG GGAACCCCTG AGCCAGCCCC ACGGGCCTCT 
CTGGAGTCTG TCCCTCCTGG CAGGTCTTAC TCACCTTATG ACTATCAGCC ATGTTTGGCT 
GGGCCTAACC AGGATTTCCA TTCAAAGAGC CCAGCCTCTT CCTCCTTGCC TGCCTTCCTT 
CCGACCACCC ACAGCCCTCC AGGGCCTCAG CAACCCCCAG CCTCTCTCCC TGGCCTCACT 
GCTCAGCCTC TGCTCTCACC AAAGGAAGCG ACTTCAGACC CCTCCCGGAC TCCAGAGGAG 
GAGCCATTGA ATTTAGAAGG GCTGGTGGCC CACAGGGTAG CAGCGTACAA CGCCAGGCTG 
CAGGGCATCG GACACAGTGG CAGCTTCCCG CCCCCTCCTC TCCACCGGCT CCAATCTTCC 
TCCCTCTCTG GGGTACAGGC TCGGGAGAAG CAGCCTGCAG AGCCCCCAGC CCCTCTGCGG 
AGGCGGGCGG CCAGTGATGG ACAGTATGAG AACCAGTCTC CAGAAGCCAC ATCCCCTCGT 
AGCCCTGGGG TTCGCTCCCC TGTCCAGTGT GTCTCCCCGG AGCTGGCTCT TACCATCGCT 
CTCAATCCTG GAGGGCGGCC CAAAGAGCCC CATTTGCACA GCTACAAGGA GGCCTTCGAG 
GAGATGGAGG GAACCTCCCC GAGCAGCCCA CCACCCAGTG GGGTGCGGTC CCCCCCGGGT 
CTGGCCAAGA CACCCCTGTC TGCTCTGGGC CTGAAACCTC ACAACCCAGC GGACATCCTG 
TTGCACCCCA CAGGAGTTAC CAGAAGACGG ATCCAGCCAG ATTCTCTTTC TCAAGAAATA 
ATCCCTGTTA ATCTCTTTCT CTCTTTTTCC TTTTTTGCTC TTCCTCATCT TCACCACCTG 
CCCTTCTTTC CTGCCTCTCC TTCCTCCCAC CTCTCTTCTC GTCTTCCCTC AGAGGAAGAT 
GAGGGGAAGG TGGTGGTCAG GCTGTCAGAA GAGCCCCGGA GCTATGTGGA GTCTGTGGCA 
CGGACAGCGG TGGCTGGACC CCGAGCTCAG GACTCTGAGC CCAAGAGCTT TAGTGCTCCA 
GCCACCCAGG CCTATGGCCA TGAGATACCC CTGAGGAACG GGACCCTGGG TGGCTCCTTT 
GTCTCCCCCA GCCCCCTCTC CACCAGCAGC CCCATCCTCA GTGCTGACAG CACTTCAGTG 
GGGAGTTTCC CGTCGGGAGA GAGCAGTGAC CAGGGTCCCC GGACGCCCAC CCAGCCTCTG 
TTGGAGTCTG GCTTCCGCTC AGGCAGCCTG GGACAGCCCA GCCCGTCTGC CCAGAGAAAC 
TACCAGAGCT CTTCTCCTCT CCCGACTGTG GGCAGTAGCT ACAGCAGCCC CGACTACTCA 
CTTCAGCATT TCAGCTCCTC TCCGGAAAGC CAGGCTCGAG CTCAGTTCAG TGTGGCTGGC 
GTCCACACGG TGCCTGGGAG CCCTCAGGCG CGCCACAGAA CAGTGGGCAC CAACACTCCC 
CCTAGTCCTG GCTTCGGCTG GCGGGCCATC AATCCCAGCA TGGCTGCCCC CAGCAGTCCC 
AGTTTGAGCC ATCACCAGAT GATGGGTCCA CCAGGCACTG GCTTCCATGG TAGCACTGTC 
TCCAGCCCCC AGAGCAGTGC AGCGACCACC CCGGGGAGCC CCAGCCTGTG TCGGCACCCA 
GCAGGGGTCT ACCAGGTTTC TGGCCTCCAC AACAAAGTGG CCACCACCCC GGGGAGTCCC 
AGCCTGGGCC GGCACCCTGG GGCTCACCAA GGCAACCTGG CCTCCGGTCT TCATAGCAAT 
GCAATAGCCA GCCCTGGAAG CCCCAGCCTG GGCCGTCACC TCGGAGGGTC TGGATCTGTG 
GTTCCCGGCA GCCCCTGCTT GGACCGGCAT GTGGCCTATG GTGGCTATTC TACCCCGGAG 
GATCGGAGAC CCACACTGTC CCGGCAGAGC AGTGCCTCTG GCTACCAGGC TCCTTCCACG 
CCCTCCTTCC CTGTCTCCCC TGCCTACTAC CCTGGCCTGA GCAGCCCTGC CACCTCCCCG 
TCACCAGACT CCGCAGCCTT CCGGCAAGGG AGCCCAACAC CAGCCTTGCC AGAGAAGCGA 
AGGATGTCAG TGGGAGACCG GGCAGGCAGC CTCCCCAACT ATGCCACCAT CAATGGGAAG 
GTGTCTTCGC CTGTCGCCAG CGGCATGTCC AGTCCCAGTG GGGGCAGCAC CGTCTCCTTC 
TCCCACACTC TGCCCGACTT CTCCAAGTAC TCCATGCCAG ACAACAGCCC GGAGACGCGG 
GCTAAAGTGA AGTTTGTCCA GGACACTTCT AAGTATTGGT ACAAGCCTGA GATCTCCAGG 
GAGCAGGCCA TCGCGCTCCT CAAGGACCAG GAGCCGGGGG CCTTCATCAT CCGCGACAGT 
CACTCCTTCC GAGGCGCGTA CGGGCTGGCC ATGAAGGTGT CTTCGCCACC TCCAACCATC 
ATGCAGCAGA ATAAAAAAGG AGACATGACC CATGAGCTGG TCAGGCATTT TCTGATAGAG 
ACTGGCCCCA GAGGAGTCAA GCTCAAGGGC TGCCCCAATG AGCCAAACTT CGGATCGCTG 
TCTGCCCTGG TCTACCAGCA CTCCATCATC CCATTGGCCC TGCCTTGCAA GCTGGTCATT 
CCAAACCGAG ACCCCACAGA TGAATCGAAA GATAGCTCCG GCCCTGCCAA CTCAACTGCA 
GACCTGCTGA AACAAGGGGC AGCCTGCAAT GTGCTCTTCG TCAACTCTGT GGACATGGAG 
TCACTCACTG GGCCACAGGC CATCTCTAAA GCCACATCTG AGACGTTGGC TGCAGACCCC 
ACGCCAGCTG CCACCATCGT TCACTTCAAA GTCTCTGCCC AGGGAATCAC TCTGACTGAC 
AACCAGAGAA AGCTCTTTTT CAGACGCCAC TACCCTCTCA ACACTGTCAC CTTCTGTGAC 
CTGGATCCAC AGGAAAGAAA GTGGATGAAA ACAGAGGGTG GTGCCCCTGC TAAGCTCTTC 
GGCTTCGTGG CCCGGAAGCA GGGCAGCACC ACGGACAACG CCTGCCACCT CTTTGCTGAG 
CTTGACCCCA ACCAGCCGGC CTCTGCCATC GTCAACTTCG TCTCCAAGGT CATGCTGAAT 
GCCGGCCAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308848.1
CDS	187..6024
Translation	MQVAAPCVPPSNHELVPITTENAPKNVVDKGEGASRGGNTRKSLEDNGSTRVTPSVQPHLQPIRNMSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCIKVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453080.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453080.1

ORF Insert Sequence:

ATGCAGGTGG CTGCCCCCTG CGTTCCTCCA TCCAACCATG AGCTGGTGCC CATCACCACT 
GAGAATGCAC CAAAGAATGT AGTGGACAAG GGAGAAGGAG CCTCCCGGGG TGGAAACACA 
CGGAAAAGCC TCGAGGACAA CGGCTCCACC AGGGTCACCC CGAGTGTCCA GCCCCACCTC 
CAGCCCATCA GAAACATGAG TGTGAGCCGG ACCATGGAGG ACAGCTGTGA GCTGGACCTG 
GTGTACGTCA CAGAGAGGAT CATCGCTGTC TCCTTCCCCA GCACAGCCAA TGAGGAGAAC 
TTCCGGAGCA ACCTCCGTGA GGTGGCGCAG ATGCTCAAGT CCAAACATGG AGGCAACTAC 
CTGCTGTTCA ACCTCTCTGA GCGGAGACCT GACATCACGA AGCTCCATGC CAAGGTACTG 
GAATTTGGCT GGCCCGACCT CCACACCCCA GCCCTGGAGA AGATCTGCAG CATCTGTAAG 
GCCATGGACA CATGGCTCAA TGCAGACCCT CACAATGTCG TTGTTCTACA CAACAAGGGA 
AACCGAGGCA GGATAGGAGT TGTCATCGCG GCTTACATGC ACTACAGCAA CATTTCTGCC 
AGTGCGGACC AGGCTCTGGA CCGGTTTGCA ATGAAGCGGT TCTATGAGGA TAAGATTGTG 
CCCATTGGCC AGCCATCCCA AAGAAGGTAC GTGCATTACT TCAGTGGCCT GCTCTCCGGC 
TCCATCAAAA TGAACAACAA GCCCTTGTTT CTGCACCACG TGATCATGCA CGGCATCCCC 
AACTTTGAGT CTAAAGGAGG ATGTCGGCCA TTTCTCCGCA TCTACCAGGC CATGCAACCT 
GTGTACACAT CTGGCATCTA CAACATCCCA GGAGACAGCC AGACTAGCGT CTGCATCACC 
ATCGAGCCAG GACTGCTCTT GAAGGGAGAC ATCTTGCTGA AGTGCTACCA CAAGAAGTTC 
CGAAGCCCAG CCCGAGACGT CATCTTCCGT GTGCAGTTCC ACACCTGTGC CATCCATGAC 
CTGGGGGTTG TCTTTGGGAA GGAGGACCTT GATGATGCTT TCAAAGATGA TCGATTTCCA 
GAGTATGGCA AAGTGGAGTT TGTATTTTCT TATGGGCCAG AGAAAATTCA AGGCATGGAG 
CACCTGGAGA ACGGGCCGAG CGTGTCTGTG GACTATAACA CCTCCGACCC CCTCATCCGC 
TGGGACTCCT ACGACAACTT CAGTGGGCAT CGAGATGACG GCATGGAGGG GGCAGTGAGA 
TCAGATTTTG ATATGAAAGC TCAAAGCTTC TCAGCTCTCT CCTTCTTCCC TGCCTTCCTC 
TCTCTGTCTG CACATTGCTT GAGAACCTTC GAGAAGCAGA TGGGCGCTCC TCATGTGATG 
CAAAGGCAGA AAGGCCCAAG GGTGGTGCCC TGGCTTCTGT CTGCCATGCA GGCCTGTGCT 
TCATGGTTTG GTGGCCTCTG CATAAAGGTG GTGGGACACA CGCAGGGGCC ACTAGATGGG 
AGCCTGTATG CTAAGGTGAA GAAGAAAGAC TCCCTGCACG GCAGCACCGG GGCTGTTAAT 
GCCACACGTC CTACACTGTC GGCCACCCCC AACCACGTGG AACACACGCT TTCTGTGAGC 
AGCGACTCGG GCAACTCCAC AGCCTCCACC AAGACCGACA AGACCGACGA GCCTGTCCCC 
GGGGCCTCCA GTGCCACTGC TGCCTTGAGT CCCCAGGAGA AGCGGGAGCT GGACCGCCTG 
CTGAGTGGCT TTGGCTTAGA GCGAGAGAAG CAAGGCGCCA TGTACCACAC CCAGCACCTC 
AGGTCCCGCC CAGCAGGGGG CTCGGCTGTG CCCTCCTCTG GACGCCACGT TGTCCCAGCC 
CAGGTTCATG TCAATGGTGG GGCGTTAGCA TCTGAGCGGG AGACAGACAT CCTGGACGAT 
GAATTGCCAA ACCAGGATGG TCACAGTGCG GGCAGCATGG GCACACTCTC TTCTCTGGAC 
GGGGTCACCA ACACCAGTGA GGGGGGCTAC CCAGAGGCCC TGTCCCCACT GACCAACGGT 
CTGGACAAGT CCTACCCCAT GGAGCCTATG GTCAATGGAG GAGGCTACCC CTACGAGTCT 
GCCAGCCGGG CGGGGCCTGC CCATGCTGGC CACACGGCCC CCATGCGGCC CTCCTACTCT 
GCACAGGAGG GTTTAGCTGG CTACCAGAGG GAGGGGCCCC ACCCAGCCTG GCCACAGCCA 
GTGACCACCT CCCACTATGC CCATGACCCC AGCGGTATGT TCCGCTCTCA ATCCTTTTCG 
GAAGCTGAAC CCCAGCTGCC CCCAGCTCCG GTCCGAGGGG GAAGCAGCCG GGAGGCTGTG 
CAAAGGGGAC TGAATTCGTG GCAGCAGCAG CAGCAGCAGC AGCAGCAGCC TCGCCCACCT 
CCACGCCAGC AGGAAAGAGC CCACTTGGAG AGTCTTGTAG CCAGCAGGCC CAGCCCTCAG 
CCATTGGCAG AGACCCCCAT CCCCAGTCTC CCTGAGTTCC CGCGAGCAGC CTCCCAGCAG 
GAGATTGAAC AGTCCATCGA AACACTCAAT ATGCTGATGC TGGACCTGGA GCCAGCCTCC 
GCTGCTGCCC CACTACACAA GTCCCAGAGT GTCCCCGGGG CCTGGCCAGG GGCTTCTCCA 
CTCTCCTCCC AGCCCCTCTC TGGATCCTCC CGTCAGTCCC ATCCACTGAC CCAGTCCAGA 
TCTGGCTATA TCCCCAGTGG GCATTCGTTG GGAACCCCTG AGCCAGCCCC ACGGGCCTCT 
CTGGAGTCTG TCCCTCCTGG CAGGTCTTAC TCACCTTATG ACTATCAGCC ATGTTTGGCT 
GGGCCTAACC AGGATTTCCA TTCAAAGAGC CCAGCCTCTT CCTCCTTGCC TGCCTTCCTT 
CCGACCACCC ACAGCCCTCC AGGGCCTCAG CAACCCCCAG CCTCTCTCCC TGGCCTCACT 
GCTCAGCCTC TGCTCTCACC AAAGGAAGCG ACTTCAGACC CCTCCCGGAC TCCAGAGGAG 
GAGCCATTGA ATTTAGAAGG GCTGGTGGCC CACAGGGTAG CAGCGTACAA CGCCAGGCTG 
CAGGGCATCG GACACAGTGG CAGCTTCCCG CCCCCTCCTC TCCACCGGCT CCAATCTTCC 
TCCCTCTCTG GGGTACAGGC TCGGGAGAAG CAGCCTGCAG AGCCCCCAGC CCCTCTGCGG 
AGGCGGGCGG CCAGTGATGG ACAGTATGAG AACCAGTCTC CAGAAGCCAC ATCCCCTCGT 
AGCCCTGGGG TTCGCTCCCC TGTCCAGTGT GTCTCCCCGG AGCTGGCTCT TACCATCGCT 
CTCAATCCTG GAGGGCGGCC CAAAGAGCCC CATTTGCACA GCTACAAGGA GGCCTTCGAG 
GAGATGGAGG GAACCTCCCC GAGCAGCCCA CCACCCAGTG GGGTGCGGTC CCCCCCGGGT 
CTGGCCAAGA CACCCCTGTC TGCTCTGGGC CTGAAACCTC ACAACCCAGC GGACATCCTG 
TTGCACCCCA CAGGAGTTAC CAGAAGACGG ATCCAGCCAG ATTCTCTTTC TCAAGAAATA 
ATCCCTGTTA ATCTCTTTCT CTCTTTTTCC TTTTTTGCTC TTCCTCATCT TCACCACCTG 
CCCTTCTTTC CTGCCTCTCC TTCCTCCCAC CTCTCTTCTC GTCTTCCCTC AGAGGAAGAT 
GAGGGGAAGG TGGTGGTCAG GCTGTCAGAA GAGCCCCGGA GCTATGTGGA GTCTGTGGCA 
CGGACAGCGG TGGCTGGACC CCGAGCTCAG GACTCTGAGC CCAAGAGCTT TAGTGCTCCA 
GCCACCCAGG CCTATGGCCA TGAGATACCC CTGAGGAACG GGACCCTGGG TGGCTCCTTT 
GTCTCCCCCA GCCCCCTCTC CACCAGCAGC CCCATCCTCA GTGCTGACAG CACTTCAGTG 
GGGAGTTTCC CGTCGGGAGA GAGCAGTGAC CAGGGTCCCC GGACGCCCAC CCAGCCTCTG 
TTGGAGTCTG GCTTCCGCTC AGGCAGCCTG GGACAGCCCA GCCCGTCTGC CCAGAGAAAC 
TACCAGAGCT CTTCTCCTCT CCCGACTGTG GGCAGTAGCT ACAGCAGCCC CGACTACTCA 
CTTCAGCATT TCAGCTCCTC TCCGGAAAGC CAGGCTCGAG CTCAGTTCAG TGTGGCTGGC 
GTCCACACGG TGCCTGGGAG CCCTCAGGCG CGCCACAGAA CAGTGGGCAC CAACACTCCC 
CCTAGTCCTG GCTTCGGCTG GCGGGCCATC AATCCCAGCA TGGCTGCCCC CAGCAGTCCC 
AGTTTGAGCC ATCACCAGAT GATGGGTCCA CCAGGCACTG GCTTCCATGG TAGCACTGTC 
TCCAGCCCCC AGAGCAGTGC AGCGACCACC CCGGGGAGCC CCAGCCTGTG TCGGCACCCA 
GCAGGGGTCT ACCAGGTTTC TGGCCTCCAC AACAAAGTGG CCACCACCCC GGGGAGTCCC 
AGCCTGGGCC GGCACCCTGG GGCTCACCAA GGCAACCTGG CCTCCGGTCT TCATAGCAAT 
GCAATAGCCA GCCCTGGAAG CCCCAGCCTG GGCCGTCACC TCGGAGGGTC TGGATCTGTG 
GTTCCCGGCA GCCCCTGCTT GGACCGGCAT GTGGCCTATG GTGGCTATTC TACCCCGGAG 
GATCGGAGAC CCACACTGTC CCGGCAGAGC AGTGCCTCTG GCTACCAGGC TCCTTCCACG 
CCCTCCTTCC CTGTCTCCCC TGCCTACTAC CCTGGCCTGA GCAGCCCTGC CACCTCCCCG 
TCACCAGACT CCGCAGCCTT CCGGCAAGGG AGCCCAACAC CAGCCTTGCC AGAGAAGCGA 
AGGATGTCAG TGGGAGACCG GGCAGGCAGC CTCCCCAACT ATGCCACCAT CAATGGGAAG 
GTGTCTTCGC CTGTCGCCAG CGGCATGTCC AGTCCCAGTG GGGGCAGCAC CGTCTCCTTC 
TCCCACACTC TGCCCGACTT CTCCAAGTAC TCCATGCCAG ACAACAGCCC GGAGACGCGG 
GCTAAAGTGA AGTTTGTCCA GGACACTTCT AAGTATTGGT ACAAGCCTGA GATCTCCAGG 
GAGCAGGCCA TCGCGCTCCT CAAGGACCAG GAGCCGGGGG CCTTCATCAT CCGCGACAGT 
CACTCCTTCC GAGGCGCGTA CGGGCTGGCC ATGAAGGTGT CTTCGCCACC TCCAACCATC 
ATGCAGCAGA ATAAAAAAGG AGACATGACC CATGAGCTGG TCAGGCATTT TCTGATAGAG 
ACTGGCCCCA GAGGAGTCAA GCTCAAGGGC TGCCCCAATG AGCCAAACTT CGGATCGCTG 
TCTGCCCTGG TCTACCAGCA CTCCATCATC CCATTGGCCC TGCCTTGCAA GCTGGTCATT 
CCAAACCGAG ACCCCACAGA TGAATCGAAA GATAGCTCCG GCCCTGCCAA CTCAACTGCA 
GACCTGCTGA AACAAGGGGC AGCCTGCAAT GTGCTCTTCG TCAACTCTGT GGACATGGAG 
TCACTCACTG GGCCACAGGC CATCTCTAAA GCCACATCTG AGACGTTGGC TGCAGACCCC 
ACGCCAGCTG CCACCATCGT TCACTTCAAA GTCTCTGCCC AGGGAATCAC TCTGACTGAC 
AACCAGAGAA AGCTCTTTTT CAGACGCCAC TACCCTCTCA ACACTGTCAC CTTCTGTGAC 
CTGGATCCAC AGGAAAGAAA GTGGATGAAA ACAGAGGGTG GTGCCCCTGC TAAGCTCTTC 
GGCTTCGTGG CCCGGAAGCA GGGCAGCACC ACGGACAACG CCTGCCACCT CTTTGCTGAG 
CTTGACCCCA ACCAGCCGGC CTCTGCCATC GTCAACTTCG TCTCCAAGGT CATGCTGAAT 
GCCGGCCAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453083.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308851.1 Homo sapiens tensin 1 (TNS1), transcript variant X16, mRNA.

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CloneID	OHu104948
Clone ID Related Accession (Same CDS sequence)	XM_024453083.1
Accession Version	XM_024453083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTGGA TCTGTCTGTC CTGCATGCTC TGGCCAGAGG ATCTGGAGGC CCCCAAGACA 
CACCGCTTCA AGGTGAAGAC CTTCAAGAAG GTGAAGCCCT GTGGGATCTG CCGCCAGGTC 
ATCACCCAGG AAGGCTGCAC CTGCAAAGTC TGCAGCTTCT CCTGTCATCG GAAATGCCAG 
GCCAAGGTGG CTGCCCCCTG CGTTCCTCCA TCCAACCATG AGCTGGTGCC CATCACCACT 
GAGAATGCAC CAAAGAATGT AGTGGACAAG GGAGAAGGAG CCTCCCGGGG TGGAAACACA 
CGGAAAAGCC TCGAGGACAA CGGCTCCACC AGGGTCACCC CGAGTGTCCA GCCCCACCTC 
CAGCCCATCA GAAACATGAG TGTGAGCCGG ACCATGGAGG ACAGCTGTGA GCTGGACCTG 
GTGTACGTCA CAGAGAGGAT CATCGCTGTC TCCTTCCCCA GCACAGCCAA TGAGGAGAAC 
TTCCGGAGCA ACCTCCGTGA GGTGGCGCAG ATGCTCAAGT CCAAACATGG AGGCAACTAC 
CTGCTGTTCA ACCTCTCTGA GCGGAGACCT GACATCACGA AGCTCCATGC CAAGGTACTG 
GAATTTGGCT GGCCCGACCT CCACACCCCA GCCCTGGAGA AGATCTGCAG CATCTGTAAG 
GCCATGGACA CATGGCTCAA TGCAGACCCT CACAATGTCG TTGTTCTACA CAACAAGGGA 
AACCGAGGCA GGATAGGAGT TGTCATCGCG GCTTACATGC ACTACAGCAA CATTTCTGCC 
AGTGCGGACC AGGCTCTGGA CCGGTTTGCA ATGAAGCGGT TCTATGAGGA TAAGATTGTG 
CCCATTGGCC AGCCATCCCA AAGAAGGTAC GTGCATTACT TCAGTGGCCT GCTCTCCGGC 
TCCATCAAAA TGAACAACAA GCCCTTGTTT CTGCACCACG TGATCATGCA CGGCATCCCC 
AACTTTGAGT CTAAAGGAGG ATGTCGGCCA TTTCTCCGCA TCTACCAGGC CATGCAACCT 
GTGTACACAT CTGGCATCTA CAACATCCCA GGAGACAGCC AGACTAGCGT CTGCATCACC 
ATCGAGCCAG GACTGCTCTT GAAGGGAGAC ATCTTGCTGA AGTGCTACCA CAAGAAGTTC 
CGAAGCCCAG CCCGAGACGT CATCTTCCGT GTGCAGTTCC ACACCTGTGC CATCCATGAC 
CTGGGGGTTG TCTTTGGGAA GGAGGACCTT GATGATGCTT TCAAAGATGA TCGATTTCCA 
GAGTATGGCA AAGTGGAGTT TGTATTTTCT TATGGGCCAG AGAAAATTCA AGGCATGGAG 
CACCTGGAGA ACGGGCCGAG CGTGTCTGTG GACTATAACA CCTCCGACCC CCTCATCCGC 
TGGGACTCCT ACGACAACTT CAGTGGGCAT CGAGATGACG GCATGGAGGA GGTGGTGGGA 
CACACGCAGG GGCCACTAGA TGGGAGCCTG TATGCTAAGG TGAAGAAGAA AGACTCCCTG 
CACGGCAGCA CCGGGGCTGT TAATGCCACA CGTCCTACAC TGTCGGCCAC CCCCAACCAC 
GTGGAACACA CGCTTTCTGT GAGCAGCGAC TCGGGCAACT CCACAGCCTC CACCAAGACC 
GACAAGACCG ACGAGCCTGT CCCCGGGGCC TCCAGTGCCA CTGCTGCCTT GAGTCCCCAG 
GAGAAGCGGG AGCTGGACCG CCTGCTGAGT GGCTTTGGCT TAGAGCGAGA GAAGCAAGGC 
GCCATGTACC ACACCCAGCA CCTCAGGTCC CGCCCAGCAG GGGGCTCGGC TGTGCCCTCC 
TCTGGACGCC ACGTTGTCCC AGCCCAGGTT CATGTCAATG GTGGGGCGTT AGCATCTGAG 
CGGGAGACAG ACATCCTGGA CGATGAATTG CCAAACCAGG ATGGTCACAG TGCGGGCAGC 
ATGGGCACAC TCTCTTCTCT GGACGGGGTC ACCAACACCA GTGAGGGGGG CTACCCAGAG 
GCCCTGTCCC CACTGACCAA CGGTCTGGAC AAGTCCTACC CCATGGAGCC TATGGTCAAT 
GGAGGAGGCT ACCCCTACGA GTCTGCCAGC CGGGCGGGGC CTGCCCATGC TGGCCACACG 
GCCCCCATGC GGCCCTCCTA CTCTGCACAG GAGGGTTTAG CTGGCTACCA GAGGGAGGGG 
CCCCACCCAG CCTGGCCACA GCCAGTGACC ACCTCCCACT ATGCCCATGA CCCCAGCGGT 
ATGTTCCGCT CTCAATCCTT TTCGGAAGCT GAACCCCAGC TGCCCCCAGC TCCGGTCCGA 
GGGGGAAGCA GCCGGGAGGC TGTGCAAAGG GGACTGAATT CGTGGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGCCTCGCCC ACCTCCACGC CAGCAGGAAA GAGCCCACTT GGAGAGTCTT 
GTAGCCAGCA GGCCCAGCCC TCAGCCATTG GCAGAGACCC CCATCCCCAG TCTCCCTGAG 
TTCCCGCGAG CAGCCTCCCA GCAGGAGATT GAACAGTCCA TCGAAACACT CAATATGCTG 
ATGCTGGACC TGGAGCCAGC CTCCGCTGCT GCCCCACTAC ACAAGTCCCA GAGTGTCCCC 
GGGGCCTGGC CAGGGGCTTC TCCACTCTCC TCCCAGCCCC TCTCTGGATC CTCCCGTCAG 
TCCCATCCAC TGACCCAGTC CAGATCTGGC TATATCCCCA GTGGGCATTC GTTGGGAACC 
CCTGAGCCAG CCCCACGGGC CTCTCTGGAG TCTGTCCCTC CTGGCAGGTC TTACTCACCT 
TATGACTATC AGCCATGTTT GGCTGGGCCT AACCAGGATT TCCATTCAAA GAGCCCAGCC 
TCTTCCTCCT TGCCTGCCTT CCTTCCGACC ACCCACAGCC CTCCAGGGCC TCAGCAACCC 
CCAGCCTCTC TCCCTGGCCT CACTGCTCAG CCTCTGCTCT CACCAAAGGA AGCGACTTCA 
GACCCCTCCC GGACTCCAGA GGAGGAGCCA TTGAATTTAG AAGGGCTGGT GGCCCACAGG 
GTAGCAGGGG TACAGGCTCG GGAGAAGCAG CCTGCAGAGC CCCCAGCCCC TCTGCGGAGG 
CGGGCGGCCA GTGATGGACA GTATGAGAAC CAGTCTCCAG AAGCCACATC CCCTCGTAGC 
CCTGGGGTTC GCTCCCCTGT CCAGTGTGTC TCCCCGGAGC TGGCTCTTAC CATCGCTCTC 
AATCCTGGAG GGCGGCCCAA AGAGCCCCAT TTGCACAGCT ACAAGGAGGC CTTCGAGGAG 
ATGGAGGGAA CCTCCCCGAG CAGCCCACCA CCCAGTGGGG TGCGGTCCCC CCCGGGTCTG 
GCCAAGACAC CCCTGTCTGC TCTGGGCCTG AAACCTCACA ACCCAGCGGA CATCCTGTTG 
CACCCCACAG GAGAGCCCCG GAGCTATGTG GAGTCTGTGG CACGGACAGC GGTGGCTGGA 
CCCCGAGCTC AGGACTCTGA GCCCAAGAGC TTTAGTGCTC CAGCCACCCA GGCCTATGGC 
CATGAGATAC CCCTGAGGAA CGGGACCCTG GGTGGCTCCT TTGTCTCCCC CAGCCCCCTC 
TCCACCAGCA GCCCCATCCT CAGTGCTGAC AGCACTTCAG TGGGGAGTTT CCCGTCGGGA 
GAGAGCAGTG ACCAGGGTCC CCGGACGCCC ACCCAGCCTC TGTTGGAGTC TGGCTTCCGC 
TCAGGCAGCC TGGGACAGCC CAGCCCGTCT GCCCAGAGAA ACTACCAGAG CTCTTCTCCT 
CTCCCGACTG TGGGCAGTAG CTACAGCAGC CCCGACTACT CACTTCAGCA TTTCAGCTCC 
TCTCCGGAAA GCCAGGCTCG AGCTCAGTTC AGTGTGGCTG GCGTCCACAC GGTGCCTGGG 
AGCCCTCAGG CGCGCCACAG AACAGTGGGC ACCAACACTC CCCCTAGTCC TGGCTTCGGC 
TGGCGGGCCA TCAATCCCAG CATGGCTGCC CCCAGCAGTC CCAGTTTGAG CCATCACCAG 
ATGATGGGTC CACCAGGCAC TGGCTTCCAT GGTAGCACTG TCTCCAGCCC CCAGAGCAGT 
GCAGCGACCA CCCCGGGGAG CCCCAGCCTG TGTCGGCACC CAGCAGGGGT CTACCAGGTT 
TCTGGCCTCC ACAACAAAGT GGCCACCACC CCGGGGAGTC CCAGCCTGGG CCGGCACCCT 
GGGGCTCACC AAGGCAACCT GGCCTCCGGT CTTCATAGCA ATGCAATAGC CAGCCCTGGA 
AGCCCCAGCC TGGGCCGTCA CCTCGGAGGG TCTGGATCTG TGGTTCCCGG CAGCCCCTGC 
TTGGACCGGC ATGTGGCCTA TGGTGGCTAT TCTACCCCGG AGGATCGGAG ACCCACACTG 
TCCCGGCAGA GCAGTGCCTC TGGCTACCAG GCTCCTTCCA CGCCCTCCTT CCCTGTCTCC 
CCTGCCTACT ACCCTGGCCT GAGCAGCCCT GCCACCTCCC CGTCACCAGA CTCCGCAGCC 
TTCCGGCAAG GGAGCCCAAC ACCAGCCTTG CCAGAGAAGC GAAGGATGTC AGTGGGAGAC 
CGGGCAGGCA GCCTCCCCAA CTATGCCACC ATCAATGGGA AGGTGTCTTC GCCTGTCGCC 
AGCGGCATGT CCAGTCCCAG TGGGGGCAGC ACCGTCTCCT TCTCCCACAC TCTGCCCGAC 
TTCTCCAAGT ACTCCATGCC AGACAACAGC CCGGAGACGC GGGCTAAAGT GAAGTTTGTC 
CAGGACACTT CTAAGTATTG GTACAAGCCT GAGATCTCCA GGGAGCAGGC CATCGCGCTC 
CTCAAGGACC AGGAGCCGGG GGCCTTCATC ATCCGCGACA GTCACTCCTT CCGAGGCGCG 
TACGGGCTGG CCATGAAGGT GTCTTCGCCA CCTCCAACCA TCATGCAGCA GAATAAAAAA 
GGAGACATGA CCCATGAGCT GGTCAGGCAT TTTCTGATAG AGACTGGCCC CAGAGGAGTC 
AAGCTCAAGG GCTGCCCCAA TGAGCCAAAC TTCGGATCGC TGTCTGCCCT GGTCTACCAG 
CACTCCATCA TCCCATTGGC CCTGCCTTGC AAGCTGGTCA TTCCAAACCG AGACCCCACA 
GATGAATCGA AAGATAGCTC CGGCCCTGCC AACTCAACTG CAGACCTGCT GAAACAAGGG 
GCAGCCTGCA ATGTGCTCTT CGTCAACTCT GTGGACATGG AGTCACTCAC TGGGCCACAG 
GCCATCTCTA AAGCCACATC TGAGACGTTG GCTGCAGACC CCACGCCAGC TGCCACCATC 
GTTCACTTCA AAGTCTCTGC CCAGGGAATC ACTCTGACTG ACAACCAGAG AAAGCTCTTT 
TTCAGACGCC ACTACCCTCT CAACACTGTC ACCTTCTGTG ACCTGGATCC ACAGGAAAGA 
AAGTGGATGA AAACAGAGGG TGGTGCCCCT GCTAAGCTCT TCGGCTTCGT GGCCCGGAAG 
CAGGGCAGCA CCACGGACAA CGCCTGCCAC CTCTTTGCTG AGCTTGACCC CAACCAGCCG 
GCCTCTGCCA TCGTCAACTT CGTCTCCAAG GTCATGCTGA ATGCCGGCCA AAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308851.1
CDS	124..5643
Translation	MTWICLSCMLWPEDLEAPKTHRFKVKTFKKVKPCGICRQVITQEGCTCKVCSFSCHRKCQAKVAAPCVPPSNHELVPITTENAPKNVVDKGEGASRGGNTRKSLEDNGSTRVTPSVQPHLQPIRNMSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453083.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453083.1

ORF Insert Sequence:

ATGACGTGGA TCTGTCTGTC CTGCATGCTC TGGCCAGAGG ATCTGGAGGC CCCCAAGACA 
CACCGCTTCA AGGTGAAGAC CTTCAAGAAG GTGAAGCCCT GTGGGATCTG CCGCCAGGTC 
ATCACCCAGG AAGGCTGCAC CTGCAAAGTC TGCAGCTTCT CCTGTCATCG GAAATGCCAG 
GCCAAGGTGG CTGCCCCCTG CGTTCCTCCA TCCAACCATG AGCTGGTGCC CATCACCACT 
GAGAATGCAC CAAAGAATGT AGTGGACAAG GGAGAAGGAG CCTCCCGGGG TGGAAACACA 
CGGAAAAGCC TCGAGGACAA CGGCTCCACC AGGGTCACCC CGAGTGTCCA GCCCCACCTC 
CAGCCCATCA GAAACATGAG TGTGAGCCGG ACCATGGAGG ACAGCTGTGA GCTGGACCTG 
GTGTACGTCA CAGAGAGGAT CATCGCTGTC TCCTTCCCCA GCACAGCCAA TGAGGAGAAC 
TTCCGGAGCA ACCTCCGTGA GGTGGCGCAG ATGCTCAAGT CCAAACATGG AGGCAACTAC 
CTGCTGTTCA ACCTCTCTGA GCGGAGACCT GACATCACGA AGCTCCATGC CAAGGTACTG 
GAATTTGGCT GGCCCGACCT CCACACCCCA GCCCTGGAGA AGATCTGCAG CATCTGTAAG 
GCCATGGACA CATGGCTCAA TGCAGACCCT CACAATGTCG TTGTTCTACA CAACAAGGGA 
AACCGAGGCA GGATAGGAGT TGTCATCGCG GCTTACATGC ACTACAGCAA CATTTCTGCC 
AGTGCGGACC AGGCTCTGGA CCGGTTTGCA ATGAAGCGGT TCTATGAGGA TAAGATTGTG 
CCCATTGGCC AGCCATCCCA AAGAAGGTAC GTGCATTACT TCAGTGGCCT GCTCTCCGGC 
TCCATCAAAA TGAACAACAA GCCCTTGTTT CTGCACCACG TGATCATGCA CGGCATCCCC 
AACTTTGAGT CTAAAGGAGG ATGTCGGCCA TTTCTCCGCA TCTACCAGGC CATGCAACCT 
GTGTACACAT CTGGCATCTA CAACATCCCA GGAGACAGCC AGACTAGCGT CTGCATCACC 
ATCGAGCCAG GACTGCTCTT GAAGGGAGAC ATCTTGCTGA AGTGCTACCA CAAGAAGTTC 
CGAAGCCCAG CCCGAGACGT CATCTTCCGT GTGCAGTTCC ACACCTGTGC CATCCATGAC 
CTGGGGGTTG TCTTTGGGAA GGAGGACCTT GATGATGCTT TCAAAGATGA TCGATTTCCA 
GAGTATGGCA AAGTGGAGTT TGTATTTTCT TATGGGCCAG AGAAAATTCA AGGCATGGAG 
CACCTGGAGA ACGGGCCGAG CGTGTCTGTG GACTATAACA CCTCCGACCC CCTCATCCGC 
TGGGACTCCT ACGACAACTT CAGTGGGCAT CGAGATGACG GCATGGAGGA GGTGGTGGGA 
CACACGCAGG GGCCACTAGA TGGGAGCCTG TATGCTAAGG TGAAGAAGAA AGACTCCCTG 
CACGGCAGCA CCGGGGCTGT TAATGCCACA CGTCCTACAC TGTCGGCCAC CCCCAACCAC 
GTGGAACACA CGCTTTCTGT GAGCAGCGAC TCGGGCAACT CCACAGCCTC CACCAAGACC 
GACAAGACCG ACGAGCCTGT CCCCGGGGCC TCCAGTGCCA CTGCTGCCTT GAGTCCCCAG 
GAGAAGCGGG AGCTGGACCG CCTGCTGAGT GGCTTTGGCT TAGAGCGAGA GAAGCAAGGC 
GCCATGTACC ACACCCAGCA CCTCAGGTCC CGCCCAGCAG GGGGCTCGGC TGTGCCCTCC 
TCTGGACGCC ACGTTGTCCC AGCCCAGGTT CATGTCAATG GTGGGGCGTT AGCATCTGAG 
CGGGAGACAG ACATCCTGGA CGATGAATTG CCAAACCAGG ATGGTCACAG TGCGGGCAGC 
ATGGGCACAC TCTCTTCTCT GGACGGGGTC ACCAACACCA GTGAGGGGGG CTACCCAGAG 
GCCCTGTCCC CACTGACCAA CGGTCTGGAC AAGTCCTACC CCATGGAGCC TATGGTCAAT 
GGAGGAGGCT ACCCCTACGA GTCTGCCAGC CGGGCGGGGC CTGCCCATGC TGGCCACACG 
GCCCCCATGC GGCCCTCCTA CTCTGCACAG GAGGGTTTAG CTGGCTACCA GAGGGAGGGG 
CCCCACCCAG CCTGGCCACA GCCAGTGACC ACCTCCCACT ATGCCCATGA CCCCAGCGGT 
ATGTTCCGCT CTCAATCCTT TTCGGAAGCT GAACCCCAGC TGCCCCCAGC TCCGGTCCGA 
GGGGGAAGCA GCCGGGAGGC TGTGCAAAGG GGACTGAATT CGTGGCAGCA GCAGCAGCAG 
CAGCAGCAGC AGCCTCGCCC ACCTCCACGC CAGCAGGAAA GAGCCCACTT GGAGAGTCTT 
GTAGCCAGCA GGCCCAGCCC TCAGCCATTG GCAGAGACCC CCATCCCCAG TCTCCCTGAG 
TTCCCGCGAG CAGCCTCCCA GCAGGAGATT GAACAGTCCA TCGAAACACT CAATATGCTG 
ATGCTGGACC TGGAGCCAGC CTCCGCTGCT GCCCCACTAC ACAAGTCCCA GAGTGTCCCC 
GGGGCCTGGC CAGGGGCTTC TCCACTCTCC TCCCAGCCCC TCTCTGGATC CTCCCGTCAG 
TCCCATCCAC TGACCCAGTC CAGATCTGGC TATATCCCCA GTGGGCATTC GTTGGGAACC 
CCTGAGCCAG CCCCACGGGC CTCTCTGGAG TCTGTCCCTC CTGGCAGGTC TTACTCACCT 
TATGACTATC AGCCATGTTT GGCTGGGCCT AACCAGGATT TCCATTCAAA GAGCCCAGCC 
TCTTCCTCCT TGCCTGCCTT CCTTCCGACC ACCCACAGCC CTCCAGGGCC TCAGCAACCC 
CCAGCCTCTC TCCCTGGCCT CACTGCTCAG CCTCTGCTCT CACCAAAGGA AGCGACTTCA 
GACCCCTCCC GGACTCCAGA GGAGGAGCCA TTGAATTTAG AAGGGCTGGT GGCCCACAGG 
GTAGCAGGGG TACAGGCTCG GGAGAAGCAG CCTGCAGAGC CCCCAGCCCC TCTGCGGAGG 
CGGGCGGCCA GTGATGGACA GTATGAGAAC CAGTCTCCAG AAGCCACATC CCCTCGTAGC 
CCTGGGGTTC GCTCCCCTGT CCAGTGTGTC TCCCCGGAGC TGGCTCTTAC CATCGCTCTC 
AATCCTGGAG GGCGGCCCAA AGAGCCCCAT TTGCACAGCT ACAAGGAGGC CTTCGAGGAG 
ATGGAGGGAA CCTCCCCGAG CAGCCCACCA CCCAGTGGGG TGCGGTCCCC CCCGGGTCTG 
GCCAAGACAC CCCTGTCTGC TCTGGGCCTG AAACCTCACA ACCCAGCGGA CATCCTGTTG 
CACCCCACAG GAGAGCCCCG GAGCTATGTG GAGTCTGTGG CACGGACAGC GGTGGCTGGA 
CCCCGAGCTC AGGACTCTGA GCCCAAGAGC TTTAGTGCTC CAGCCACCCA GGCCTATGGC 
CATGAGATAC CCCTGAGGAA CGGGACCCTG GGTGGCTCCT TTGTCTCCCC CAGCCCCCTC 
TCCACCAGCA GCCCCATCCT CAGTGCTGAC AGCACTTCAG TGGGGAGTTT CCCGTCGGGA 
GAGAGCAGTG ACCAGGGTCC CCGGACGCCC ACCCAGCCTC TGTTGGAGTC TGGCTTCCGC 
TCAGGCAGCC TGGGACAGCC CAGCCCGTCT GCCCAGAGAA ACTACCAGAG CTCTTCTCCT 
CTCCCGACTG TGGGCAGTAG CTACAGCAGC CCCGACTACT CACTTCAGCA TTTCAGCTCC 
TCTCCGGAAA GCCAGGCTCG AGCTCAGTTC AGTGTGGCTG GCGTCCACAC GGTGCCTGGG 
AGCCCTCAGG CGCGCCACAG AACAGTGGGC ACCAACACTC CCCCTAGTCC TGGCTTCGGC 
TGGCGGGCCA TCAATCCCAG CATGGCTGCC CCCAGCAGTC CCAGTTTGAG CCATCACCAG 
ATGATGGGTC CACCAGGCAC TGGCTTCCAT GGTAGCACTG TCTCCAGCCC CCAGAGCAGT 
GCAGCGACCA CCCCGGGGAG CCCCAGCCTG TGTCGGCACC CAGCAGGGGT CTACCAGGTT 
TCTGGCCTCC ACAACAAAGT GGCCACCACC CCGGGGAGTC CCAGCCTGGG CCGGCACCCT 
GGGGCTCACC AAGGCAACCT GGCCTCCGGT CTTCATAGCA ATGCAATAGC CAGCCCTGGA 
AGCCCCAGCC TGGGCCGTCA CCTCGGAGGG TCTGGATCTG TGGTTCCCGG CAGCCCCTGC 
TTGGACCGGC ATGTGGCCTA TGGTGGCTAT TCTACCCCGG AGGATCGGAG ACCCACACTG 
TCCCGGCAGA GCAGTGCCTC TGGCTACCAG GCTCCTTCCA CGCCCTCCTT CCCTGTCTCC 
CCTGCCTACT ACCCTGGCCT GAGCAGCCCT GCCACCTCCC CGTCACCAGA CTCCGCAGCC 
TTCCGGCAAG GGAGCCCAAC ACCAGCCTTG CCAGAGAAGC GAAGGATGTC AGTGGGAGAC 
CGGGCAGGCA GCCTCCCCAA CTATGCCACC ATCAATGGGA AGGTGTCTTC GCCTGTCGCC 
AGCGGCATGT CCAGTCCCAG TGGGGGCAGC ACCGTCTCCT TCTCCCACAC TCTGCCCGAC 
TTCTCCAAGT ACTCCATGCC AGACAACAGC CCGGAGACGC GGGCTAAAGT GAAGTTTGTC 
CAGGACACTT CTAAGTATTG GTACAAGCCT GAGATCTCCA GGGAGCAGGC CATCGCGCTC 
CTCAAGGACC AGGAGCCGGG GGCCTTCATC ATCCGCGACA GTCACTCCTT CCGAGGCGCG 
TACGGGCTGG CCATGAAGGT GTCTTCGCCA CCTCCAACCA TCATGCAGCA GAATAAAAAA 
GGAGACATGA CCCATGAGCT GGTCAGGCAT TTTCTGATAG AGACTGGCCC CAGAGGAGTC 
AAGCTCAAGG GCTGCCCCAA TGAGCCAAAC TTCGGATCGC TGTCTGCCCT GGTCTACCAG 
CACTCCATCA TCCCATTGGC CCTGCCTTGC AAGCTGGTCA TTCCAAACCG AGACCCCACA 
GATGAATCGA AAGATAGCTC CGGCCCTGCC AACTCAACTG CAGACCTGCT GAAACAAGGG 
GCAGCCTGCA ATGTGCTCTT CGTCAACTCT GTGGACATGG AGTCACTCAC TGGGCCACAG 
GCCATCTCTA AAGCCACATC TGAGACGTTG GCTGCAGACC CCACGCCAGC TGCCACCATC 
GTTCACTTCA AAGTCTCTGC CCAGGGAATC ACTCTGACTG ACAACCAGAG AAAGCTCTTT 
TTCAGACGCC ACTACCCTCT CAACACTGTC ACCTTCTGTG ACCTGGATCC ACAGGAAAGA 
AAGTGGATGA AAACAGAGGG TGGTGCCCCT GCTAAGCTCT TCGGCTTCGT GGCCCGGAAG 
CAGGGCAGCA CCACGGACAA CGCCTGCCAC CTCTTTGCTG AGCTTGACCC CAACCAGCCG 
GCCTCTGCCA TCGTCAACTT CGTCTCCAAG GTCATGCTGA ATGCCGGCCA AAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453070.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308838.1 Homo sapiens tensin 1 (TNS1), transcript variant X3, mRNA.

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CloneID	OHu61082
Clone ID Related Accession (Same CDS sequence)	XM_024453070.1
Accession Version	XM_024453070.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGCG TACAACGCCA GGCTGCAGGG CATCGGACAC 
AGTGGCAGCT TCCCGCCCCC TCCTCTCCAC CGGCTCCAAT CTTCCTCCCT CTCTGGGGTA 
CAGGCTCGGG AGAAGCAGCC TGCAGAGCCC CCAGCCCCTC TGCGGAGGCG GGCGGCCAGT 
GATGGACAGT ATGAGAACCA GTCTCCAGAA GCCACATCCC CTCGTAGCCC TGGGGTTCGC 
TCCCCTGTCC AGTGTGTCTC CCCGGAGCTG GCTCTTACCA TCGCTCTCAA TCCTGGAGGG 
CGGCCCAAAG AGCCCCATTT GCACAGCTAC AAGGAGGCCT TCGAGGAGAT GGAGGGAACC 
TCCCCGAGCA GCCCACCACC CAGTGGGGTG CGGTCCCCCC CGGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCTCACAAC CCAGCGGACA TCCTGTTGCA CCCCACAGGA 
GTTACCAGAA GACGGATCCA GCCAGAGGAA GATGAGGGGA AGGTGGTGGT CAGGCTGTCA 
GAAGAGCCCC GGAGCTATGT GGAGTCTGTG GCACGGACAG CGGTGGCTGG ACCCCGAGCT 
CAGGACTCTG AGCCCAAGAG CTTTAGTGCT CCAGCCACCC AGGCCTATGG CCATGAGATA 
CCCCTGAGGA ACGGGACCCT GGGTGGCTCC TTTGTCTCCC CCAGCCCCCT CTCCACCAGC 
AGCCCCATCC TCAGTGCTGA CAGCACTTCA GTGGGGAGTT TCCCGTCGGG AGAGAGCAGT 
GACCAGGGTC CCCGGACGCC CACCCAGCCT CTGTTGGAGT CTGGCTTCCG CTCAGGCAGC 
CTGGGACAGC CCAGCCCGTC TGCCCAGAGA AACTACCAGA GCTCTTCTCC TCTCCCGACT 
GTGGGCAGTA GCTACAGCAG CCCCGACTAC TCACTTCAGC ATTTCAGCTC CTCTCCGGAA 
AGCCAGGCTC GAGCTCAGTT CAGTGTGGCT GGCGTCCACA CGGTGCCTGG GAGCCCTCAG 
GCGCGCCACA GAACAGTGGG CACCAACACT CCCCCTAGTC CTGGCTTCGG CTGGCGGGCC 
ATCAATCCCA GCATGGCTGC CCCCAGCAGT CCCAGTTTGA GCCATCACCA GATGATGGGT 
CCACCAGGCA CTGGCTTCCA TGGTAGCACT GTCTCCAGCC CCCAGAGCAG TGCAGCGACC 
ACCCCGGGGA GCCCCAGCCT GTGTCGGCAC CCAGCAGGGG TCTACCAGGT TTCTGGCCTC 
CACAACAAAG TGGCCACCAC CCCGGGGAGT CCCAGCCTGG GCCGGCACCC TGGGGCTCAC 
CAAGGCAACC TGGCCTCCGG TCTTCATAGC AATGCAATAG CCAGCCCTGG AAGCCCCAGC 
CTGGGCCGTC ACCTCGGAGG GTCTGGATCT GTGGTTCCCG GCAGCCCCTG CTTGGACCGG 
CATGTGGCCT ATGGTGGCTA TTCTACCCCG GAGGATCGGA GACCCACACT GTCCCGGCAG 
AGCAGTGCCT CTGGCTACCA GGCTCCTTCC ACGCCCTCCT TCCCTGTCTC CCCTGCCTAC 
TACCCTGGCC TGAGCAGCCC TGCCACCTCC CCGTCACCAG ACTCCGCAGC CTTCCGGCAA 
GGGAGCCCAA CACCAGCCTT GCCAGAGAAG CGAAGGATGT CAGTGGGAGA CCGGGCAGGC 
AGCCTCCCCA ACTATGCCAC CATCAATGGG AAGGTGTCTT CGCCTGTCGC CAGCGGCATG 
TCCAGTCCCA GTGGGGGCAG CACCGTCTCC TTCTCCCACA CTCTGCCCGA CTTCTCCAAG 
TACTCCATGC CAGACAACAG CCCGGAGACG CGGGCTAAAG TGAAGTTTGT CCAGGACACT 
TCTAAGTATT GGTACAAGCC TGAGATCTCC AGGGAGCAGG CCATCGCGCT CCTCAAGGAC 
CAGGAGCCGG GGGCCTTCAT CATCCGCGAC AGTCACTCCT TCCGAGGCGC GTACGGGCTG 
GCCATGAAGG TGTCTTCGCC ACCTCCAACC ATCATGCAGC AGAATAAAAA AGGAGACATG 
ACCCATGAGC TGGTCAGGCA TTTTCTGATA GAGACTGGCC CCAGAGGAGT CAAGCTCAAG 
GGCTGCCCCA ATGAGCCAAA CTTCGGATCG CTGTCTGCCC TGGTCTACCA GCACTCCATC 
ATCCCATTGG CCCTGCCTTG CAAGCTGGTC ATTCCAAACC GAGACCCCAC AGATGAATCG 
AAAGATAGCT CCGGCCCTGC CAACTCAACT GCAGACCTGC TGAAACAAGG GGCAGCCTGC 
AATGTGCTCT TCGTCAACTC TGTGGACATG GAGTCACTCA CTGGGCCACA GGCCATCTCT 
AAAGCCACAT CTGAGACGTT GGCTGCAGAC CCCACGCCAG CTGCCACCAT CGTTCACTTC 
AAAGTCTCTG CCCAGGGAAT CACTCTGACT GACAACCAGA GAAAGCTCTT TTTCAGACGC 
CACTACCCTC TCAACACTGT CACCTTCTGT GACCTGGATC CACAGGAAAG AAAGTGGATG 
AAAACAGAGG GTGGTGCCCC TGCTAAGCTC TTCGGCTTCG TGGCCCGGAA GCAGGGCAGC 
ACCACGGACA ACGCCTGCCA CCTCTTTGCT GAGCTTGACC CCAACCAGCC GGCCTCTGCC 
ATCGTCAACT TCGTCTCCAA GGTCATGCTG AATGCCGGCC AAAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308838.1
CDS	190..5700
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCIKVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453070.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453070.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGCG TACAACGCCA GGCTGCAGGG CATCGGACAC 
AGTGGCAGCT TCCCGCCCCC TCCTCTCCAC CGGCTCCAAT CTTCCTCCCT CTCTGGGGTA 
CAGGCTCGGG AGAAGCAGCC TGCAGAGCCC CCAGCCCCTC TGCGGAGGCG GGCGGCCAGT 
GATGGACAGT ATGAGAACCA GTCTCCAGAA GCCACATCCC CTCGTAGCCC TGGGGTTCGC 
TCCCCTGTCC AGTGTGTCTC CCCGGAGCTG GCTCTTACCA TCGCTCTCAA TCCTGGAGGG 
CGGCCCAAAG AGCCCCATTT GCACAGCTAC AAGGAGGCCT TCGAGGAGAT GGAGGGAACC 
TCCCCGAGCA GCCCACCACC CAGTGGGGTG CGGTCCCCCC CGGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCTCACAAC CCAGCGGACA TCCTGTTGCA CCCCACAGGA 
GTTACCAGAA GACGGATCCA GCCAGAGGAA GATGAGGGGA AGGTGGTGGT CAGGCTGTCA 
GAAGAGCCCC GGAGCTATGT GGAGTCTGTG GCACGGACAG CGGTGGCTGG ACCCCGAGCT 
CAGGACTCTG AGCCCAAGAG CTTTAGTGCT CCAGCCACCC AGGCCTATGG CCATGAGATA 
CCCCTGAGGA ACGGGACCCT GGGTGGCTCC TTTGTCTCCC CCAGCCCCCT CTCCACCAGC 
AGCCCCATCC TCAGTGCTGA CAGCACTTCA GTGGGGAGTT TCCCGTCGGG AGAGAGCAGT 
GACCAGGGTC CCCGGACGCC CACCCAGCCT CTGTTGGAGT CTGGCTTCCG CTCAGGCAGC 
CTGGGACAGC CCAGCCCGTC TGCCCAGAGA AACTACCAGA GCTCTTCTCC TCTCCCGACT 
GTGGGCAGTA GCTACAGCAG CCCCGACTAC TCACTTCAGC ATTTCAGCTC CTCTCCGGAA 
AGCCAGGCTC GAGCTCAGTT CAGTGTGGCT GGCGTCCACA CGGTGCCTGG GAGCCCTCAG 
GCGCGCCACA GAACAGTGGG CACCAACACT CCCCCTAGTC CTGGCTTCGG CTGGCGGGCC 
ATCAATCCCA GCATGGCTGC CCCCAGCAGT CCCAGTTTGA GCCATCACCA GATGATGGGT 
CCACCAGGCA CTGGCTTCCA TGGTAGCACT GTCTCCAGCC CCCAGAGCAG TGCAGCGACC 
ACCCCGGGGA GCCCCAGCCT GTGTCGGCAC CCAGCAGGGG TCTACCAGGT TTCTGGCCTC 
CACAACAAAG TGGCCACCAC CCCGGGGAGT CCCAGCCTGG GCCGGCACCC TGGGGCTCAC 
CAAGGCAACC TGGCCTCCGG TCTTCATAGC AATGCAATAG CCAGCCCTGG AAGCCCCAGC 
CTGGGCCGTC ACCTCGGAGG GTCTGGATCT GTGGTTCCCG GCAGCCCCTG CTTGGACCGG 
CATGTGGCCT ATGGTGGCTA TTCTACCCCG GAGGATCGGA GACCCACACT GTCCCGGCAG 
AGCAGTGCCT CTGGCTACCA GGCTCCTTCC ACGCCCTCCT TCCCTGTCTC CCCTGCCTAC 
TACCCTGGCC TGAGCAGCCC TGCCACCTCC CCGTCACCAG ACTCCGCAGC CTTCCGGCAA 
GGGAGCCCAA CACCAGCCTT GCCAGAGAAG CGAAGGATGT CAGTGGGAGA CCGGGCAGGC 
AGCCTCCCCA ACTATGCCAC CATCAATGGG AAGGTGTCTT CGCCTGTCGC CAGCGGCATG 
TCCAGTCCCA GTGGGGGCAG CACCGTCTCC TTCTCCCACA CTCTGCCCGA CTTCTCCAAG 
TACTCCATGC CAGACAACAG CCCGGAGACG CGGGCTAAAG TGAAGTTTGT CCAGGACACT 
TCTAAGTATT GGTACAAGCC TGAGATCTCC AGGGAGCAGG CCATCGCGCT CCTCAAGGAC 
CAGGAGCCGG GGGCCTTCAT CATCCGCGAC AGTCACTCCT TCCGAGGCGC GTACGGGCTG 
GCCATGAAGG TGTCTTCGCC ACCTCCAACC ATCATGCAGC AGAATAAAAA AGGAGACATG 
ACCCATGAGC TGGTCAGGCA TTTTCTGATA GAGACTGGCC CCAGAGGAGT CAAGCTCAAG 
GGCTGCCCCA ATGAGCCAAA CTTCGGATCG CTGTCTGCCC TGGTCTACCA GCACTCCATC 
ATCCCATTGG CCCTGCCTTG CAAGCTGGTC ATTCCAAACC GAGACCCCAC AGATGAATCG 
AAAGATAGCT CCGGCCCTGC CAACTCAACT GCAGACCTGC TGAAACAAGG GGCAGCCTGC 
AATGTGCTCT TCGTCAACTC TGTGGACATG GAGTCACTCA CTGGGCCACA GGCCATCTCT 
AAAGCCACAT CTGAGACGTT GGCTGCAGAC CCCACGCCAG CTGCCACCAT CGTTCACTTC 
AAAGTCTCTG CCCAGGGAAT CACTCTGACT GACAACCAGA GAAAGCTCTT TTTCAGACGC 
CACTACCCTC TCAACACTGT CACCTTCTGT GACCTGGATC CACAGGAAAG AAAGTGGATG 
AAAACAGAGG GTGGTGCCCC TGCTAAGCTC TTCGGCTTCG TGGCCCGGAA GCAGGGCAGC 
ACCACGGACA ACGCCTGCCA CCTCTTTGCT GAGCTTGACC CCAACCAGCC GGCCTCTGCC 
ATCGTCAACT TCGTCTCCAA GGTCATGCTG AATGCCGGCC AAAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004801.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860290.1 Homo sapiens tensin 1 (TNS1), transcript variant X1, mRNA.

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CloneID	OHu89834
Clone ID Related Accession (Same CDS sequence)	XM_017004801.1
Accession Version	XM_017004801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGCGTA CAACGCCAGG CTGCAGGGCA TCGGACACAG TGGCAGCTTC 
CCGCCCCCTC CTCTCCACCG GCTCCAATCT TCCTCCCTCT CTGGGGTACA GGCTCGGGAG 
AAGCAGCCTG CAGAGCCCCC AGCCCCTCTG CGGAGGCGGG CGGCCAGTGA TGGACAGTAT 
GAGAACCAGT CTCCAGAAGC CACATCCCCT CGTAGCCCTG GGGTTCGCTC CCCTGTCCAG 
TGTGTCTCCC CGGAGCTGGC TCTTACCATC GCTCTCAATC CTGGAGGGCG GCCCAAAGAG 
CCCCATTTGC ACAGCTACAA GGAGGCCTTC GAGGAGATGG AGGGAACCTC CCCGAGCAGC 
CCACCACCCA GTGGGGTGCG GTCCCCCCCG GGTCTGGCCA AGACACCCCT GTCTGCTCTG 
GGCCTGAAAC CTCACAACCC AGCGGACATC CTGTTGCACC CCACAGGAGT TACCAGAAGA 
CGGATCCAGC CAGATTCTCT TTCTCAAGAA ATAATCCCTG TTAATCTCTT TCTCTCTTTT 
TCCTTTTTTG CTCTTCCTCA TCTTCACCAC CTGCCCTTCT TTCCTGCCTC TCCTTCCTCC 
CACCTCTCTT CTCGTCTTCC CTCAGAGGAA GATGAGGGGA AGGTGGTGGT CAGGCTGTCA 
GAAGAGCCCC GGAGCTATGT GGAGTCTGTG GCACGGACAG CGGTGGCTGG ACCCCGAGCT 
CAGGACTCTG AGCCCAAGAG CTTTAGTGCT CCAGCCACCC AGGCCTATGG CCATGAGATA 
CCCCTGAGGA ACGGGACCCT GGGTGGCTCC TTTGTCTCCC CCAGCCCCCT CTCCACCAGC 
AGCCCCATCC TCAGTGCTGA CAGCACTTCA GTGGGGAGTT TCCCGTCGGG AGAGAGCAGT 
GACCAGGGTC CCCGGACGCC CACCCAGCCT CTGTTGGAGT CTGGCTTCCG CTCAGGCAGC 
CTGGGACAGC CCAGCCCGTC TGCCCAGAGA AACTACCAGA GCTCTTCTCC TCTCCCGACT 
GTGGGCAGTA GCTACAGCAG CCCCGACTAC TCACTTCAGC ATTTCAGCTC CTCTCCGGAA 
AGCCAGGCTC GAGCTCAGTT CAGTGTGGCT GGCGTCCACA CGGTGCCTGG GAGCCCTCAG 
GCGCGCCACA GAACAGTGGG CACCAACACT CCCCCTAGTC CTGGCTTCGG CTGGCGGGCC 
ATCAATCCCA GCATGGCTGC CCCCAGCAGT CCCAGTTTGA GCCATCACCA GATGATGGGT 
CCACCAGGCA CTGGCTTCCA TGGTAGCACT GTCTCCAGCC CCCAGAGCAG TGCAGCGACC 
ACCCCGGGGA GCCCCAGCCT GTGTCGGCAC CCAGCAGGGG TCTACCAGGT TTCTGGCCTC 
CACAACAAAG TGGCCACCAC CCCGGGGAGT CCCAGCCTGG GCCGGCACCC TGGGGCTCAC 
CAAGGCAACC TGGCCTCCGG TCTTCATAGC AATGCAATAG CCAGCCCTGG AAGCCCCAGC 
CTGGGCCGTC ACCTCGGAGG GTCTGGATCT GTGGTTCCCG GCAGCCCCTG CTTGGACCGG 
CATGTGGCCT ATGGTGGCTA TTCTACCCCG GAGGATCGGA GACCCACACT GTCCCGGCAG 
AGCAGTGCCT CTGGCTACCA GGCTCCTTCC ACGCCCTCCT TCCCTGTCTC CCCTGCCTAC 
TACCCTGGCC TGAGCAGCCC TGCCACCTCC CCGTCACCAG ACTCCGCAGC CTTCCGGCAA 
GGGAGCCCAA CACCAGCCTT GCCAGAGAAG CGAAGGATGT CAGTGGGAGA CCGGGCAGGC 
AGCCTCCCCA ACTATGCCAC CATCAATGGG AAGGTGTCTT CGCCTGTCGC CAGCGGCATG 
TCCAGTCCCA GTGGGGGCAG CACCGTCTCC TTCTCCCACA CTCTGCCCGA CTTCTCCAAG 
TACTCCATGC CAGACAACAG CCCGGAGACG CGGGCTAAAG TGAAGTTTGT CCAGGACACT 
TCTAAGTATT GGTACAAGCC TGAGATCTCC AGGGAGCAGG CCATCGCGCT CCTCAAGGAC 
CAGGAGCCGG GGGCCTTCAT CATCCGCGAC AGTCACTCCT TCCGAGGCGC GTACGGGCTG 
GCCATGAAGG TGTCTTCGCC ACCTCCAACC ATCATGCAGC AGAATAAAAA AGACATGACC 
CATGAGCTGG TCAGGCATTT TCTGATAGAG ACTGGCCCCA GAGGAGTCAA GCTCAAGGGC 
TGCCCCAATG AGCCAAACTT CGGATCGCTG TCTGCCCTGG TCTACCAGCA CTCCATCATC 
CCATTGGCCC TGCCTTGCAA GCTGGTCATT CCAAACCGAG ACCCCACAGA TGAATCGAAA 
GATAGCTCCG GCCCTGCCAA CTCAACTGCA GACCTGCTGA AACAAGGGGC AGCCTGCAAT 
GTGCTCTTCG TCAACTCTGT GGACATGGAG TCACTCACTG GGCCACAGGC CATCTCTAAA 
GCCACATCTG AGACGTTGGC TGCAGACCCC ACGCCAGCTG CCACCATCGT TCACTTCAAA 
GTCTCTGCCC AGGGAATCAC TCTGACTGAC AACCAGAGAA AGCTCTTTTT CAGACGCCAC 
TACCCTCTCA ACACTGTCAC CTTCTGTGAC CTGGATCCAC AGGAAAGAAA GTGGATGAAA 
ACAGAGGGTG GTGCCCCTGC TAAGCTCTTC GGCTTCGTGG CCCGGAAGCA GGGCAGCACC 
ACGGACAACG CCTGCCACCT CTTTGCTGAG CTTGACCCCA ACCAGCCGGC CTCTGCCATC 
GTCAACTTCG TCTCCAAGGT CATGCTGAAT GCCGGCCAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860290.1
CDS	55..5742
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCINGNKQNTNSQSIGSISEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004801.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004801.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGCGTA CAACGCCAGG CTGCAGGGCA TCGGACACAG TGGCAGCTTC 
CCGCCCCCTC CTCTCCACCG GCTCCAATCT TCCTCCCTCT CTGGGGTACA GGCTCGGGAG 
AAGCAGCCTG CAGAGCCCCC AGCCCCTCTG CGGAGGCGGG CGGCCAGTGA TGGACAGTAT 
GAGAACCAGT CTCCAGAAGC CACATCCCCT CGTAGCCCTG GGGTTCGCTC CCCTGTCCAG 
TGTGTCTCCC CGGAGCTGGC TCTTACCATC GCTCTCAATC CTGGAGGGCG GCCCAAAGAG 
CCCCATTTGC ACAGCTACAA GGAGGCCTTC GAGGAGATGG AGGGAACCTC CCCGAGCAGC 
CCACCACCCA GTGGGGTGCG GTCCCCCCCG GGTCTGGCCA AGACACCCCT GTCTGCTCTG 
GGCCTGAAAC CTCACAACCC AGCGGACATC CTGTTGCACC CCACAGGAGT TACCAGAAGA 
CGGATCCAGC CAGATTCTCT TTCTCAAGAA ATAATCCCTG TTAATCTCTT TCTCTCTTTT 
TCCTTTTTTG CTCTTCCTCA TCTTCACCAC CTGCCCTTCT TTCCTGCCTC TCCTTCCTCC 
CACCTCTCTT CTCGTCTTCC CTCAGAGGAA GATGAGGGGA AGGTGGTGGT CAGGCTGTCA 
GAAGAGCCCC GGAGCTATGT GGAGTCTGTG GCACGGACAG CGGTGGCTGG ACCCCGAGCT 
CAGGACTCTG AGCCCAAGAG CTTTAGTGCT CCAGCCACCC AGGCCTATGG CCATGAGATA 
CCCCTGAGGA ACGGGACCCT GGGTGGCTCC TTTGTCTCCC CCAGCCCCCT CTCCACCAGC 
AGCCCCATCC TCAGTGCTGA CAGCACTTCA GTGGGGAGTT TCCCGTCGGG AGAGAGCAGT 
GACCAGGGTC CCCGGACGCC CACCCAGCCT CTGTTGGAGT CTGGCTTCCG CTCAGGCAGC 
CTGGGACAGC CCAGCCCGTC TGCCCAGAGA AACTACCAGA GCTCTTCTCC TCTCCCGACT 
GTGGGCAGTA GCTACAGCAG CCCCGACTAC TCACTTCAGC ATTTCAGCTC CTCTCCGGAA 
AGCCAGGCTC GAGCTCAGTT CAGTGTGGCT GGCGTCCACA CGGTGCCTGG GAGCCCTCAG 
GCGCGCCACA GAACAGTGGG CACCAACACT CCCCCTAGTC CTGGCTTCGG CTGGCGGGCC 
ATCAATCCCA GCATGGCTGC CCCCAGCAGT CCCAGTTTGA GCCATCACCA GATGATGGGT 
CCACCAGGCA CTGGCTTCCA TGGTAGCACT GTCTCCAGCC CCCAGAGCAG TGCAGCGACC 
ACCCCGGGGA GCCCCAGCCT GTGTCGGCAC CCAGCAGGGG TCTACCAGGT TTCTGGCCTC 
CACAACAAAG TGGCCACCAC CCCGGGGAGT CCCAGCCTGG GCCGGCACCC TGGGGCTCAC 
CAAGGCAACC TGGCCTCCGG TCTTCATAGC AATGCAATAG CCAGCCCTGG AAGCCCCAGC 
CTGGGCCGTC ACCTCGGAGG GTCTGGATCT GTGGTTCCCG GCAGCCCCTG CTTGGACCGG 
CATGTGGCCT ATGGTGGCTA TTCTACCCCG GAGGATCGGA GACCCACACT GTCCCGGCAG 
AGCAGTGCCT CTGGCTACCA GGCTCCTTCC ACGCCCTCCT TCCCTGTCTC CCCTGCCTAC 
TACCCTGGCC TGAGCAGCCC TGCCACCTCC CCGTCACCAG ACTCCGCAGC CTTCCGGCAA 
GGGAGCCCAA CACCAGCCTT GCCAGAGAAG CGAAGGATGT CAGTGGGAGA CCGGGCAGGC 
AGCCTCCCCA ACTATGCCAC CATCAATGGG AAGGTGTCTT CGCCTGTCGC CAGCGGCATG 
TCCAGTCCCA GTGGGGGCAG CACCGTCTCC TTCTCCCACA CTCTGCCCGA CTTCTCCAAG 
TACTCCATGC CAGACAACAG CCCGGAGACG CGGGCTAAAG TGAAGTTTGT CCAGGACACT 
TCTAAGTATT GGTACAAGCC TGAGATCTCC AGGGAGCAGG CCATCGCGCT CCTCAAGGAC 
CAGGAGCCGG GGGCCTTCAT CATCCGCGAC AGTCACTCCT TCCGAGGCGC GTACGGGCTG 
GCCATGAAGG TGTCTTCGCC ACCTCCAACC ATCATGCAGC AGAATAAAAA AGACATGACC 
CATGAGCTGG TCAGGCATTT TCTGATAGAG ACTGGCCCCA GAGGAGTCAA GCTCAAGGGC 
TGCCCCAATG AGCCAAACTT CGGATCGCTG TCTGCCCTGG TCTACCAGCA CTCCATCATC 
CCATTGGCCC TGCCTTGCAA GCTGGTCATT CCAAACCGAG ACCCCACAGA TGAATCGAAA 
GATAGCTCCG GCCCTGCCAA CTCAACTGCA GACCTGCTGA AACAAGGGGC AGCCTGCAAT 
GTGCTCTTCG TCAACTCTGT GGACATGGAG TCACTCACTG GGCCACAGGC CATCTCTAAA 
GCCACATCTG AGACGTTGGC TGCAGACCCC ACGCCAGCTG CCACCATCGT TCACTTCAAA 
GTCTCTGCCC AGGGAATCAC TCTGACTGAC AACCAGAGAA AGCTCTTTTT CAGACGCCAC 
TACCCTCTCA ACACTGTCAC CTTCTGTGAC CTGGATCCAC AGGAAAGAAA GTGGATGAAA 
ACAGAGGGTG GTGCCCCTGC TAAGCTCTTC GGCTTCGTGG CCCGGAAGCA GGGCAGCACC 
ACGGACAACG CCTGCCACCT CTTTGCTGAG CTTGACCCCA ACCAGCCGGC CTCTGCCATC 
GTCAACTTCG TCTCCAAGGT CATGCTGAAT GCCGGCCAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453081.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308849.1 Homo sapiens tensin 1 (TNS1), transcript variant X14, mRNA.

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CloneID	OHu89835
Clone ID Related Accession (Same CDS sequence)	XM_024453081.1 , XM_017004802.1 , XM_024453082.1
Accession Version	XM_024453081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGCG TACAACGCCA GGCTGCAGGG CATCGGACAC 
AGTGGCAGCT TCCCGCCCCC TCCTCTCCAC CGGCTCCAAT CTTCCTCCCT CTCTGGGGTA 
CAGGCTCGGG AGAAGCAGCC TGCAGAGCCC CCAGCCCCTC TGCGGAGGCG GGCGGCCAGT 
GATGGACAGT ATGAGAACCA GTCTCCAGAA GCCACATCCC CTCGTAGCCC TGGGGTTCGC 
TCCCCTGTCC AGTGTGTCTC CCCGGAGCTG GCTCTTACCA TCGCTCTCAA TCCTGGAGGG 
CGGCCCAAAG AGCCCCATTT GCACAGCTAC AAGGAGGCCT TCGAGGAGAT GGAGGGAACC 
TCCCCGAGCA GCCCACCACC CAGTGGGGTG CGGTCCCCCC CGGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCTCACAAC CCAGCGGACA TCCTGTTGCA CCCCACAGGA 
GTTACCAGAA GACGGATCCA GCCAGATTCT CTTTCTCAAG AAATAATCCC TGTTAATCTC 
TTTCTCTCTT TTTCCTTTTT TGCTCTTCCT CATCTTCACC ACCTGCCCTT CTTTCCTGCC 
TCTCCTTCCT CCCACCTCTC TTCTCGTCTT CCCTCAGAGG AAGATGAGGG GAAGGTGGTG 
GTCAGGCTGT CAGAAGAGCC CCGGAGCTAT GTGGAGTCTG TGGCACGGAC AGCGGTGGCT 
GGACCCCGAG CTCAGGACTC TGAGCCCAAG AGCTTTAGTG CTCCAGCCAC CCAGGCCTAT 
GGCCATGAGA TACCCCTGAG GAACGGGACC CTGGGTGGCT CCTTTGTCTC CCCCAGCCCC 
CTCTCCACCA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CAGTGGGGAG TTTCCCGTCG 
GGAGAGAGCA GTGACCAGGG TCCCCGGACG CCCACCCAGC CTCTGTTGGA GTCTGGCTTC 
CGCTCAGGCA GCCTGGGACA GCCCAGCCCG TCTGCCCAGA GAAACTACCA GAGCTCTTCT 
CCTCTCCCGA CTGTGGGCAG TAGCTACAGC AGCCCCGACT ACTCACTTCA GCATTTCAGC 
TCCTCTCCGG AAAGCCAGGC TCGAGCTCAG TTCAGTGTGG CTGGCGTCCA CACGGTGCCT 
GGGAGCCCTC AGGCGCGCCA CAGAACAGTG GGCACCAACA CTCCCCCTAG TCCTGGCTTC 
GGCTGGCGGG CCATCAATCC CAGCATGGCT GCCCCCAGCA GTCCCAGTTT GAGCCATCAC 
CAGATGATGG GTCCACCAGG CACTGGCTTC CATGGTAGCA CTGTCTCCAG CCCCCAGAGC 
AGTGCAGCGA CCACCCCGGG GAGCCCCAGC CTGTGTCGGC ACCCAGCAGG GGTCTACCAG 
GTTTCTGGCC TCCACAACAA AGTGGCCACC ACCCCGGGGA GTCCCAGCCT GGGCCGGCAC 
CCTGGGGCTC ACCAAGGCAA CCTGGCCTCC GGTCTTCATA GCAATGCAAT AGCCAGCCCT 
GGAAGCCCCA GCCTGGGCCG TCACCTCGGA GGGTCTGGAT CTGTGGTTCC CGGCAGCCCC 
TGCTTGGACC GGCATGTGGC CTATGGTGGC TATTCTACCC CGGAGGATCG GAGACCCACA 
CTGTCCCGGC AGAGCAGTGC CTCTGGCTAC CAGGCTCCTT CCACGCCCTC CTTCCCTGTC 
TCCCCTGCCT ACTACCCTGG CCTGAGCAGC CCTGCCACCT CCCCGTCACC AGACTCCGCA 
GCCTTCCGGC AAGGGAGCCC AACACCAGCC TTGCCAGAGA AGCGAAGGAT GTCAGTGGGA 
GACCGGGCAG GCAGCCTCCC CAACTATGCC ACCATCAATG GGAAGGTGTC TTCGCCTGTC 
GCCAGCGGCA TGTCCAGTCC CAGTGGGGGC AGCACCGTCT CCTTCTCCCA CACTCTGCCC 
GACTTCTCCA AGTACTCCAT GCCAGACAAC AGCCCGGAGA CGCGGGCTAA AGTGAAGTTT 
GTCCAGGACA CTTCTAAGTA TTGGTACAAG CCTGAGATCT CCAGGGAGCA GGCCATCGCG 
CTCCTCAAGG ACCAGGAGCC GGGGGCCTTC ATCATCCGCG ACAGTCACTC CTTCCGAGGC 
GCGTACGGGC TGGCCATGAA GGTGTCTTCG CCACCTCCAA CCATCATGCA GCAGAATAAA 
AAAGGAGACA TGACCCATGA GCTGGTCAGG CATTTTCTGA TAGAGACTGG CCCCAGAGGA 
GTCAAGCTCA AGGGCTGCCC CAATGAGCCA AACTTCGGAT CGCTGTCTGC CCTGGTCTAC 
CAGCACTCCA TCATCCCATT GGCCCTGCCT TGCAAGCTGG TCATTCCAAA CCGAGACCCC 
ACAGATGAAT CGAAAGATAG CTCCGGCCCT GCCAACTCAA CTGCAGACCT GCTGAAACAA 
GGGGCAGCCT GCAATGTGCT CTTCGTCAAC TCTGTGGACA TGGAGTCACT CACTGGGCCA 
CAGGCCATCT CTAAAGCCAC ATCTGAGACG TTGGCTGCAG ACCCCACGCC AGCTGCCACC 
ATCGTTCACT TCAAAGTCTC TGCCCAGGGA ATCACTCTGA CTGACAACCA GAGAAAGCTC 
TTTTTCAGAC GCCACTACCC TCTCAACACT GTCACCTTCT GTGACCTGGA TCCACAGGAA 
AGAAAGTGGA TGAAAACAGA GGGTGGTGCC CCTGCTAAGC TCTTCGGCTT CGTGGCCCGG 
AAGCAGGGCA GCACCACGGA CAACGCCTGC CACCTCTTTG CTGAGCTTGA CCCCAACCAG 
CCGGCCTCTG CCATCGTCAA CTTCGTCTCC AAGGTCATGC TGAATGCCGG CCAAAAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308849.1
CDS	396..6038
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCIKVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453081.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453081.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGCG TACAACGCCA GGCTGCAGGG CATCGGACAC 
AGTGGCAGCT TCCCGCCCCC TCCTCTCCAC CGGCTCCAAT CTTCCTCCCT CTCTGGGGTA 
CAGGCTCGGG AGAAGCAGCC TGCAGAGCCC CCAGCCCCTC TGCGGAGGCG GGCGGCCAGT 
GATGGACAGT ATGAGAACCA GTCTCCAGAA GCCACATCCC CTCGTAGCCC TGGGGTTCGC 
TCCCCTGTCC AGTGTGTCTC CCCGGAGCTG GCTCTTACCA TCGCTCTCAA TCCTGGAGGG 
CGGCCCAAAG AGCCCCATTT GCACAGCTAC AAGGAGGCCT TCGAGGAGAT GGAGGGAACC 
TCCCCGAGCA GCCCACCACC CAGTGGGGTG CGGTCCCCCC CGGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCTCACAAC CCAGCGGACA TCCTGTTGCA CCCCACAGGA 
GTTACCAGAA GACGGATCCA GCCAGATTCT CTTTCTCAAG AAATAATCCC TGTTAATCTC 
TTTCTCTCTT TTTCCTTTTT TGCTCTTCCT CATCTTCACC ACCTGCCCTT CTTTCCTGCC 
TCTCCTTCCT CCCACCTCTC TTCTCGTCTT CCCTCAGAGG AAGATGAGGG GAAGGTGGTG 
GTCAGGCTGT CAGAAGAGCC CCGGAGCTAT GTGGAGTCTG TGGCACGGAC AGCGGTGGCT 
GGACCCCGAG CTCAGGACTC TGAGCCCAAG AGCTTTAGTG CTCCAGCCAC CCAGGCCTAT 
GGCCATGAGA TACCCCTGAG GAACGGGACC CTGGGTGGCT CCTTTGTCTC CCCCAGCCCC 
CTCTCCACCA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CAGTGGGGAG TTTCCCGTCG 
GGAGAGAGCA GTGACCAGGG TCCCCGGACG CCCACCCAGC CTCTGTTGGA GTCTGGCTTC 
CGCTCAGGCA GCCTGGGACA GCCCAGCCCG TCTGCCCAGA GAAACTACCA GAGCTCTTCT 
CCTCTCCCGA CTGTGGGCAG TAGCTACAGC AGCCCCGACT ACTCACTTCA GCATTTCAGC 
TCCTCTCCGG AAAGCCAGGC TCGAGCTCAG TTCAGTGTGG CTGGCGTCCA CACGGTGCCT 
GGGAGCCCTC AGGCGCGCCA CAGAACAGTG GGCACCAACA CTCCCCCTAG TCCTGGCTTC 
GGCTGGCGGG CCATCAATCC CAGCATGGCT GCCCCCAGCA GTCCCAGTTT GAGCCATCAC 
CAGATGATGG GTCCACCAGG CACTGGCTTC CATGGTAGCA CTGTCTCCAG CCCCCAGAGC 
AGTGCAGCGA CCACCCCGGG GAGCCCCAGC CTGTGTCGGC ACCCAGCAGG GGTCTACCAG 
GTTTCTGGCC TCCACAACAA AGTGGCCACC ACCCCGGGGA GTCCCAGCCT GGGCCGGCAC 
CCTGGGGCTC ACCAAGGCAA CCTGGCCTCC GGTCTTCATA GCAATGCAAT AGCCAGCCCT 
GGAAGCCCCA GCCTGGGCCG TCACCTCGGA GGGTCTGGAT CTGTGGTTCC CGGCAGCCCC 
TGCTTGGACC GGCATGTGGC CTATGGTGGC TATTCTACCC CGGAGGATCG GAGACCCACA 
CTGTCCCGGC AGAGCAGTGC CTCTGGCTAC CAGGCTCCTT CCACGCCCTC CTTCCCTGTC 
TCCCCTGCCT ACTACCCTGG CCTGAGCAGC CCTGCCACCT CCCCGTCACC AGACTCCGCA 
GCCTTCCGGC AAGGGAGCCC AACACCAGCC TTGCCAGAGA AGCGAAGGAT GTCAGTGGGA 
GACCGGGCAG GCAGCCTCCC CAACTATGCC ACCATCAATG GGAAGGTGTC TTCGCCTGTC 
GCCAGCGGCA TGTCCAGTCC CAGTGGGGGC AGCACCGTCT CCTTCTCCCA CACTCTGCCC 
GACTTCTCCA AGTACTCCAT GCCAGACAAC AGCCCGGAGA CGCGGGCTAA AGTGAAGTTT 
GTCCAGGACA CTTCTAAGTA TTGGTACAAG CCTGAGATCT CCAGGGAGCA GGCCATCGCG 
CTCCTCAAGG ACCAGGAGCC GGGGGCCTTC ATCATCCGCG ACAGTCACTC CTTCCGAGGC 
GCGTACGGGC TGGCCATGAA GGTGTCTTCG CCACCTCCAA CCATCATGCA GCAGAATAAA 
AAAGGAGACA TGACCCATGA GCTGGTCAGG CATTTTCTGA TAGAGACTGG CCCCAGAGGA 
GTCAAGCTCA AGGGCTGCCC CAATGAGCCA AACTTCGGAT CGCTGTCTGC CCTGGTCTAC 
CAGCACTCCA TCATCCCATT GGCCCTGCCT TGCAAGCTGG TCATTCCAAA CCGAGACCCC 
ACAGATGAAT CGAAAGATAG CTCCGGCCCT GCCAACTCAA CTGCAGACCT GCTGAAACAA 
GGGGCAGCCT GCAATGTGCT CTTCGTCAAC TCTGTGGACA TGGAGTCACT CACTGGGCCA 
CAGGCCATCT CTAAAGCCAC ATCTGAGACG TTGGCTGCAG ACCCCACGCC AGCTGCCACC 
ATCGTTCACT TCAAAGTCTC TGCCCAGGGA ATCACTCTGA CTGACAACCA GAGAAAGCTC 
TTTTTCAGAC GCCACTACCC TCTCAACACT GTCACCTTCT GTGACCTGGA TCCACAGGAA 
AGAAAGTGGA TGAAAACAGA GGGTGGTGCC CCTGCTAAGC TCTTCGGCTT CGTGGCCCGG 
AAGCAGGGCA GCACCACGGA CAACGCCTGC CACCTCTTTG CTGAGCTTGA CCCCAACCAG 
CCGGCCTCTG CCATCGTCAA CTTCGTCTCC AAGGTCATGC TGAATGCCGG CCAAAAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004802.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860291.1 Homo sapiens tensin 1 (TNS1), transcript variant X2, mRNA.

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CloneID	OHu89835
Clone ID Related Accession (Same CDS sequence)	XM_024453081.1 , XM_017004802.1 , XM_024453082.1
Accession Version	XM_017004802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGCG TACAACGCCA GGCTGCAGGG CATCGGACAC 
AGTGGCAGCT TCCCGCCCCC TCCTCTCCAC CGGCTCCAAT CTTCCTCCCT CTCTGGGGTA 
CAGGCTCGGG AGAAGCAGCC TGCAGAGCCC CCAGCCCCTC TGCGGAGGCG GGCGGCCAGT 
GATGGACAGT ATGAGAACCA GTCTCCAGAA GCCACATCCC CTCGTAGCCC TGGGGTTCGC 
TCCCCTGTCC AGTGTGTCTC CCCGGAGCTG GCTCTTACCA TCGCTCTCAA TCCTGGAGGG 
CGGCCCAAAG AGCCCCATTT GCACAGCTAC AAGGAGGCCT TCGAGGAGAT GGAGGGAACC 
TCCCCGAGCA GCCCACCACC CAGTGGGGTG CGGTCCCCCC CGGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCTCACAAC CCAGCGGACA TCCTGTTGCA CCCCACAGGA 
GTTACCAGAA GACGGATCCA GCCAGATTCT CTTTCTCAAG AAATAATCCC TGTTAATCTC 
TTTCTCTCTT TTTCCTTTTT TGCTCTTCCT CATCTTCACC ACCTGCCCTT CTTTCCTGCC 
TCTCCTTCCT CCCACCTCTC TTCTCGTCTT CCCTCAGAGG AAGATGAGGG GAAGGTGGTG 
GTCAGGCTGT CAGAAGAGCC CCGGAGCTAT GTGGAGTCTG TGGCACGGAC AGCGGTGGCT 
GGACCCCGAG CTCAGGACTC TGAGCCCAAG AGCTTTAGTG CTCCAGCCAC CCAGGCCTAT 
GGCCATGAGA TACCCCTGAG GAACGGGACC CTGGGTGGCT CCTTTGTCTC CCCCAGCCCC 
CTCTCCACCA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CAGTGGGGAG TTTCCCGTCG 
GGAGAGAGCA GTGACCAGGG TCCCCGGACG CCCACCCAGC CTCTGTTGGA GTCTGGCTTC 
CGCTCAGGCA GCCTGGGACA GCCCAGCCCG TCTGCCCAGA GAAACTACCA GAGCTCTTCT 
CCTCTCCCGA CTGTGGGCAG TAGCTACAGC AGCCCCGACT ACTCACTTCA GCATTTCAGC 
TCCTCTCCGG AAAGCCAGGC TCGAGCTCAG TTCAGTGTGG CTGGCGTCCA CACGGTGCCT 
GGGAGCCCTC AGGCGCGCCA CAGAACAGTG GGCACCAACA CTCCCCCTAG TCCTGGCTTC 
GGCTGGCGGG CCATCAATCC CAGCATGGCT GCCCCCAGCA GTCCCAGTTT GAGCCATCAC 
CAGATGATGG GTCCACCAGG CACTGGCTTC CATGGTAGCA CTGTCTCCAG CCCCCAGAGC 
AGTGCAGCGA CCACCCCGGG GAGCCCCAGC CTGTGTCGGC ACCCAGCAGG GGTCTACCAG 
GTTTCTGGCC TCCACAACAA AGTGGCCACC ACCCCGGGGA GTCCCAGCCT GGGCCGGCAC 
CCTGGGGCTC ACCAAGGCAA CCTGGCCTCC GGTCTTCATA GCAATGCAAT AGCCAGCCCT 
GGAAGCCCCA GCCTGGGCCG TCACCTCGGA GGGTCTGGAT CTGTGGTTCC CGGCAGCCCC 
TGCTTGGACC GGCATGTGGC CTATGGTGGC TATTCTACCC CGGAGGATCG GAGACCCACA 
CTGTCCCGGC AGAGCAGTGC CTCTGGCTAC CAGGCTCCTT CCACGCCCTC CTTCCCTGTC 
TCCCCTGCCT ACTACCCTGG CCTGAGCAGC CCTGCCACCT CCCCGTCACC AGACTCCGCA 
GCCTTCCGGC AAGGGAGCCC AACACCAGCC TTGCCAGAGA AGCGAAGGAT GTCAGTGGGA 
GACCGGGCAG GCAGCCTCCC CAACTATGCC ACCATCAATG GGAAGGTGTC TTCGCCTGTC 
GCCAGCGGCA TGTCCAGTCC CAGTGGGGGC AGCACCGTCT CCTTCTCCCA CACTCTGCCC 
GACTTCTCCA AGTACTCCAT GCCAGACAAC AGCCCGGAGA CGCGGGCTAA AGTGAAGTTT 
GTCCAGGACA CTTCTAAGTA TTGGTACAAG CCTGAGATCT CCAGGGAGCA GGCCATCGCG 
CTCCTCAAGG ACCAGGAGCC GGGGGCCTTC ATCATCCGCG ACAGTCACTC CTTCCGAGGC 
GCGTACGGGC TGGCCATGAA GGTGTCTTCG CCACCTCCAA CCATCATGCA GCAGAATAAA 
AAAGGAGACA TGACCCATGA GCTGGTCAGG CATTTTCTGA TAGAGACTGG CCCCAGAGGA 
GTCAAGCTCA AGGGCTGCCC CAATGAGCCA AACTTCGGAT CGCTGTCTGC CCTGGTCTAC 
CAGCACTCCA TCATCCCATT GGCCCTGCCT TGCAAGCTGG TCATTCCAAA CCGAGACCCC 
ACAGATGAAT CGAAAGATAG CTCCGGCCCT GCCAACTCAA CTGCAGACCT GCTGAAACAA 
GGGGCAGCCT GCAATGTGCT CTTCGTCAAC TCTGTGGACA TGGAGTCACT CACTGGGCCA 
CAGGCCATCT CTAAAGCCAC ATCTGAGACG TTGGCTGCAG ACCCCACGCC AGCTGCCACC 
ATCGTTCACT TCAAAGTCTC TGCCCAGGGA ATCACTCTGA CTGACAACCA GAGAAAGCTC 
TTTTTCAGAC GCCACTACCC TCTCAACACT GTCACCTTCT GTGACCTGGA TCCACAGGAA 
AGAAAGTGGA TGAAAACAGA GGGTGGTGCC CCTGCTAAGC TCTTCGGCTT CGTGGCCCGG 
AAGCAGGGCA GCACCACGGA CAACGCCTGC CACCTCTTTG CTGAGCTTGA CCCCAACCAG 
CCGGCCTCTG CCATCGTCAA CTTCGTCTCC AAGGTCATGC TGAATGCCGG CCAAAAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860291.1
CDS	55..5697
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCIKVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004802.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004802.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGCG TACAACGCCA GGCTGCAGGG CATCGGACAC 
AGTGGCAGCT TCCCGCCCCC TCCTCTCCAC CGGCTCCAAT CTTCCTCCCT CTCTGGGGTA 
CAGGCTCGGG AGAAGCAGCC TGCAGAGCCC CCAGCCCCTC TGCGGAGGCG GGCGGCCAGT 
GATGGACAGT ATGAGAACCA GTCTCCAGAA GCCACATCCC CTCGTAGCCC TGGGGTTCGC 
TCCCCTGTCC AGTGTGTCTC CCCGGAGCTG GCTCTTACCA TCGCTCTCAA TCCTGGAGGG 
CGGCCCAAAG AGCCCCATTT GCACAGCTAC AAGGAGGCCT TCGAGGAGAT GGAGGGAACC 
TCCCCGAGCA GCCCACCACC CAGTGGGGTG CGGTCCCCCC CGGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCTCACAAC CCAGCGGACA TCCTGTTGCA CCCCACAGGA 
GTTACCAGAA GACGGATCCA GCCAGATTCT CTTTCTCAAG AAATAATCCC TGTTAATCTC 
TTTCTCTCTT TTTCCTTTTT TGCTCTTCCT CATCTTCACC ACCTGCCCTT CTTTCCTGCC 
TCTCCTTCCT CCCACCTCTC TTCTCGTCTT CCCTCAGAGG AAGATGAGGG GAAGGTGGTG 
GTCAGGCTGT CAGAAGAGCC CCGGAGCTAT GTGGAGTCTG TGGCACGGAC AGCGGTGGCT 
GGACCCCGAG CTCAGGACTC TGAGCCCAAG AGCTTTAGTG CTCCAGCCAC CCAGGCCTAT 
GGCCATGAGA TACCCCTGAG GAACGGGACC CTGGGTGGCT CCTTTGTCTC CCCCAGCCCC 
CTCTCCACCA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CAGTGGGGAG TTTCCCGTCG 
GGAGAGAGCA GTGACCAGGG TCCCCGGACG CCCACCCAGC CTCTGTTGGA GTCTGGCTTC 
CGCTCAGGCA GCCTGGGACA GCCCAGCCCG TCTGCCCAGA GAAACTACCA GAGCTCTTCT 
CCTCTCCCGA CTGTGGGCAG TAGCTACAGC AGCCCCGACT ACTCACTTCA GCATTTCAGC 
TCCTCTCCGG AAAGCCAGGC TCGAGCTCAG TTCAGTGTGG CTGGCGTCCA CACGGTGCCT 
GGGAGCCCTC AGGCGCGCCA CAGAACAGTG GGCACCAACA CTCCCCCTAG TCCTGGCTTC 
GGCTGGCGGG CCATCAATCC CAGCATGGCT GCCCCCAGCA GTCCCAGTTT GAGCCATCAC 
CAGATGATGG GTCCACCAGG CACTGGCTTC CATGGTAGCA CTGTCTCCAG CCCCCAGAGC 
AGTGCAGCGA CCACCCCGGG GAGCCCCAGC CTGTGTCGGC ACCCAGCAGG GGTCTACCAG 
GTTTCTGGCC TCCACAACAA AGTGGCCACC ACCCCGGGGA GTCCCAGCCT GGGCCGGCAC 
CCTGGGGCTC ACCAAGGCAA CCTGGCCTCC GGTCTTCATA GCAATGCAAT AGCCAGCCCT 
GGAAGCCCCA GCCTGGGCCG TCACCTCGGA GGGTCTGGAT CTGTGGTTCC CGGCAGCCCC 
TGCTTGGACC GGCATGTGGC CTATGGTGGC TATTCTACCC CGGAGGATCG GAGACCCACA 
CTGTCCCGGC AGAGCAGTGC CTCTGGCTAC CAGGCTCCTT CCACGCCCTC CTTCCCTGTC 
TCCCCTGCCT ACTACCCTGG CCTGAGCAGC CCTGCCACCT CCCCGTCACC AGACTCCGCA 
GCCTTCCGGC AAGGGAGCCC AACACCAGCC TTGCCAGAGA AGCGAAGGAT GTCAGTGGGA 
GACCGGGCAG GCAGCCTCCC CAACTATGCC ACCATCAATG GGAAGGTGTC TTCGCCTGTC 
GCCAGCGGCA TGTCCAGTCC CAGTGGGGGC AGCACCGTCT CCTTCTCCCA CACTCTGCCC 
GACTTCTCCA AGTACTCCAT GCCAGACAAC AGCCCGGAGA CGCGGGCTAA AGTGAAGTTT 
GTCCAGGACA CTTCTAAGTA TTGGTACAAG CCTGAGATCT CCAGGGAGCA GGCCATCGCG 
CTCCTCAAGG ACCAGGAGCC GGGGGCCTTC ATCATCCGCG ACAGTCACTC CTTCCGAGGC 
GCGTACGGGC TGGCCATGAA GGTGTCTTCG CCACCTCCAA CCATCATGCA GCAGAATAAA 
AAAGGAGACA TGACCCATGA GCTGGTCAGG CATTTTCTGA TAGAGACTGG CCCCAGAGGA 
GTCAAGCTCA AGGGCTGCCC CAATGAGCCA AACTTCGGAT CGCTGTCTGC CCTGGTCTAC 
CAGCACTCCA TCATCCCATT GGCCCTGCCT TGCAAGCTGG TCATTCCAAA CCGAGACCCC 
ACAGATGAAT CGAAAGATAG CTCCGGCCCT GCCAACTCAA CTGCAGACCT GCTGAAACAA 
GGGGCAGCCT GCAATGTGCT CTTCGTCAAC TCTGTGGACA TGGAGTCACT CACTGGGCCA 
CAGGCCATCT CTAAAGCCAC ATCTGAGACG TTGGCTGCAG ACCCCACGCC AGCTGCCACC 
ATCGTTCACT TCAAAGTCTC TGCCCAGGGA ATCACTCTGA CTGACAACCA GAGAAAGCTC 
TTTTTCAGAC GCCACTACCC TCTCAACACT GTCACCTTCT GTGACCTGGA TCCACAGGAA 
AGAAAGTGGA TGAAAACAGA GGGTGGTGCC CCTGCTAAGC TCTTCGGCTT CGTGGCCCGG 
AAGCAGGGCA GCACCACGGA CAACGCCTGC CACCTCTTTG CTGAGCTTGA CCCCAACCAG 
CCGGCCTCTG CCATCGTCAA CTTCGTCTCC AAGGTCATGC TGAATGCCGG CCAAAAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453082.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308850.1 Homo sapiens tensin 1 (TNS1), transcript variant X15, mRNA.

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CloneID	OHu89835
Clone ID Related Accession (Same CDS sequence)	XM_024453081.1 , XM_017004802.1 , XM_024453082.1
Accession Version	XM_024453082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGCG TACAACGCCA GGCTGCAGGG CATCGGACAC 
AGTGGCAGCT TCCCGCCCCC TCCTCTCCAC CGGCTCCAAT CTTCCTCCCT CTCTGGGGTA 
CAGGCTCGGG AGAAGCAGCC TGCAGAGCCC CCAGCCCCTC TGCGGAGGCG GGCGGCCAGT 
GATGGACAGT ATGAGAACCA GTCTCCAGAA GCCACATCCC CTCGTAGCCC TGGGGTTCGC 
TCCCCTGTCC AGTGTGTCTC CCCGGAGCTG GCTCTTACCA TCGCTCTCAA TCCTGGAGGG 
CGGCCCAAAG AGCCCCATTT GCACAGCTAC AAGGAGGCCT TCGAGGAGAT GGAGGGAACC 
TCCCCGAGCA GCCCACCACC CAGTGGGGTG CGGTCCCCCC CGGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCTCACAAC CCAGCGGACA TCCTGTTGCA CCCCACAGGA 
GTTACCAGAA GACGGATCCA GCCAGATTCT CTTTCTCAAG AAATAATCCC TGTTAATCTC 
TTTCTCTCTT TTTCCTTTTT TGCTCTTCCT CATCTTCACC ACCTGCCCTT CTTTCCTGCC 
TCTCCTTCCT CCCACCTCTC TTCTCGTCTT CCCTCAGAGG AAGATGAGGG GAAGGTGGTG 
GTCAGGCTGT CAGAAGAGCC CCGGAGCTAT GTGGAGTCTG TGGCACGGAC AGCGGTGGCT 
GGACCCCGAG CTCAGGACTC TGAGCCCAAG AGCTTTAGTG CTCCAGCCAC CCAGGCCTAT 
GGCCATGAGA TACCCCTGAG GAACGGGACC CTGGGTGGCT CCTTTGTCTC CCCCAGCCCC 
CTCTCCACCA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CAGTGGGGAG TTTCCCGTCG 
GGAGAGAGCA GTGACCAGGG TCCCCGGACG CCCACCCAGC CTCTGTTGGA GTCTGGCTTC 
CGCTCAGGCA GCCTGGGACA GCCCAGCCCG TCTGCCCAGA GAAACTACCA GAGCTCTTCT 
CCTCTCCCGA CTGTGGGCAG TAGCTACAGC AGCCCCGACT ACTCACTTCA GCATTTCAGC 
TCCTCTCCGG AAAGCCAGGC TCGAGCTCAG TTCAGTGTGG CTGGCGTCCA CACGGTGCCT 
GGGAGCCCTC AGGCGCGCCA CAGAACAGTG GGCACCAACA CTCCCCCTAG TCCTGGCTTC 
GGCTGGCGGG CCATCAATCC CAGCATGGCT GCCCCCAGCA GTCCCAGTTT GAGCCATCAC 
CAGATGATGG GTCCACCAGG CACTGGCTTC CATGGTAGCA CTGTCTCCAG CCCCCAGAGC 
AGTGCAGCGA CCACCCCGGG GAGCCCCAGC CTGTGTCGGC ACCCAGCAGG GGTCTACCAG 
GTTTCTGGCC TCCACAACAA AGTGGCCACC ACCCCGGGGA GTCCCAGCCT GGGCCGGCAC 
CCTGGGGCTC ACCAAGGCAA CCTGGCCTCC GGTCTTCATA GCAATGCAAT AGCCAGCCCT 
GGAAGCCCCA GCCTGGGCCG TCACCTCGGA GGGTCTGGAT CTGTGGTTCC CGGCAGCCCC 
TGCTTGGACC GGCATGTGGC CTATGGTGGC TATTCTACCC CGGAGGATCG GAGACCCACA 
CTGTCCCGGC AGAGCAGTGC CTCTGGCTAC CAGGCTCCTT CCACGCCCTC CTTCCCTGTC 
TCCCCTGCCT ACTACCCTGG CCTGAGCAGC CCTGCCACCT CCCCGTCACC AGACTCCGCA 
GCCTTCCGGC AAGGGAGCCC AACACCAGCC TTGCCAGAGA AGCGAAGGAT GTCAGTGGGA 
GACCGGGCAG GCAGCCTCCC CAACTATGCC ACCATCAATG GGAAGGTGTC TTCGCCTGTC 
GCCAGCGGCA TGTCCAGTCC CAGTGGGGGC AGCACCGTCT CCTTCTCCCA CACTCTGCCC 
GACTTCTCCA AGTACTCCAT GCCAGACAAC AGCCCGGAGA CGCGGGCTAA AGTGAAGTTT 
GTCCAGGACA CTTCTAAGTA TTGGTACAAG CCTGAGATCT CCAGGGAGCA GGCCATCGCG 
CTCCTCAAGG ACCAGGAGCC GGGGGCCTTC ATCATCCGCG ACAGTCACTC CTTCCGAGGC 
GCGTACGGGC TGGCCATGAA GGTGTCTTCG CCACCTCCAA CCATCATGCA GCAGAATAAA 
AAAGGAGACA TGACCCATGA GCTGGTCAGG CATTTTCTGA TAGAGACTGG CCCCAGAGGA 
GTCAAGCTCA AGGGCTGCCC CAATGAGCCA AACTTCGGAT CGCTGTCTGC CCTGGTCTAC 
CAGCACTCCA TCATCCCATT GGCCCTGCCT TGCAAGCTGG TCATTCCAAA CCGAGACCCC 
ACAGATGAAT CGAAAGATAG CTCCGGCCCT GCCAACTCAA CTGCAGACCT GCTGAAACAA 
GGGGCAGCCT GCAATGTGCT CTTCGTCAAC TCTGTGGACA TGGAGTCACT CACTGGGCCA 
CAGGCCATCT CTAAAGCCAC ATCTGAGACG TTGGCTGCAG ACCCCACGCC AGCTGCCACC 
ATCGTTCACT TCAAAGTCTC TGCCCAGGGA ATCACTCTGA CTGACAACCA GAGAAAGCTC 
TTTTTCAGAC GCCACTACCC TCTCAACACT GTCACCTTCT GTGACCTGGA TCCACAGGAA 
AGAAAGTGGA TGAAAACAGA GGGTGGTGCC CCTGCTAAGC TCTTCGGCTT CGTGGCCCGG 
AAGCAGGGCA GCACCACGGA CAACGCCTGC CACCTCTTTG CTGAGCTTGA CCCCAACCAG 
CCGGCCTCTG CCATCGTCAA CTTCGTCTCC AAGGTCATGC TGAATGCCGG CCAAAAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308850.1
CDS	520..6162
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCIKVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453082.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453082.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA AGGTGGTGGG ACACACGCAG GGGCCACTAG ATGGGAGCCT GTATGCTAAG 
GTGAAGAAGA AAGACTCCCT GCACGGCAGC ACCGGGGCTG TTAATGCCAC ACGTCCTACA 
CTGTCGGCCA CCCCCAACCA CGTGGAACAC ACGCTTTCTG TGAGCAGCGA CTCGGGCAAC 
TCCACAGCCT CCACCAAGAC CGACAAGACC GACGAGCCTG TCCCCGGGGC CTCCAGTGCC 
ACTGCTGCCT TGAGTCCCCA GGAGAAGCGG GAGCTGGACC GCCTGCTGAG TGGCTTTGGC 
TTAGAGCGAG AGAAGCAAGG CGCCATGTAC CACACCCAGC ACCTCAGGTC CCGCCCAGCA 
GGGGGCTCGG CTGTGCCCTC CTCTGGACGC CACGTTGTCC CAGCCCAGGT TCATGTCAAT 
GGTGGGGCGT TAGCATCTGA GCGGGAGACA GACATCCTGG ACGATGAATT GCCAAACCAG 
GATGGTCACA GTGCGGGCAG CATGGGCACA CTCTCTTCTC TGGACGGGGT CACCAACACC 
AGTGAGGGGG GCTACCCAGA GGCCCTGTCC CCACTGACCA ACGGTCTGGA CAAGTCCTAC 
CCCATGGAGC CTATGGTCAA TGGAGGAGGC TACCCCTACG AGTCTGCCAG CCGGGCGGGG 
CCTGCCCATG CTGGCCACAC GGCCCCCATG CGGCCCTCCT ACTCTGCACA GGAGGGTTTA 
GCTGGCTACC AGAGGGAGGG GCCCCACCCA GCCTGGCCAC AGCCAGTGAC CACCTCCCAC 
TATGCCCATG ACCCCAGCGG TATGTTCCGC TCTCAATCCT TTTCGGAAGC TGAACCCCAG 
CTGCCCCCAG CTCCGGTCCG AGGGGGAAGC AGCCGGGAGG CTGTGCAAAG GGGACTGAAT 
TCGTGGCAGC AGCAGCAGCA GCAGCAGCAG CAGCCTCGCC CACCTCCACG CCAGCAGGAA 
AGAGCCCACT TGGAGAGTCT TGTAGCCAGC AGGCCCAGCC CTCAGCCATT GGCAGAGACC 
CCCATCCCCA GTCTCCCTGA GTTCCCGCGA GCAGCCTCCC AGCAGGAGAT TGAACAGTCC 
ATCGAAACAC TCAATATGCT GATGCTGGAC CTGGAGCCAG CCTCCGCTGC TGCCCCACTA 
CACAAGTCCC AGAGTGTCCC CGGGGCCTGG CCAGGGGCTT CTCCACTCTC CTCCCAGCCC 
CTCTCTGGAT CCTCCCGTCA GTCCCATCCA CTGACCCAGT CCAGATCTGG CTATATCCCC 
AGTGGGCATT CGTTGGGAAC CCCTGAGCCA GCCCCACGGG CCTCTCTGGA GTCTGTCCCT 
CCTGGCAGGT CTTACTCACC TTATGACTAT CAGCCATGTT TGGCTGGGCC TAACCAGGAT 
TTCCATTCAA AGAGCCCAGC CTCTTCCTCC TTGCCTGCCT TCCTTCCGAC CACCCACAGC 
CCTCCAGGGC CTCAGCAACC CCCAGCCTCT CTCCCTGGCC TCACTGCTCA GCCTCTGCTC 
TCACCAAAGG AAGCGACTTC AGACCCCTCC CGGACTCCAG AGGAGGAGCC ATTGAATTTA 
GAAGGGCTGG TGGCCCACAG GGTAGCAGCG TACAACGCCA GGCTGCAGGG CATCGGACAC 
AGTGGCAGCT TCCCGCCCCC TCCTCTCCAC CGGCTCCAAT CTTCCTCCCT CTCTGGGGTA 
CAGGCTCGGG AGAAGCAGCC TGCAGAGCCC CCAGCCCCTC TGCGGAGGCG GGCGGCCAGT 
GATGGACAGT ATGAGAACCA GTCTCCAGAA GCCACATCCC CTCGTAGCCC TGGGGTTCGC 
TCCCCTGTCC AGTGTGTCTC CCCGGAGCTG GCTCTTACCA TCGCTCTCAA TCCTGGAGGG 
CGGCCCAAAG AGCCCCATTT GCACAGCTAC AAGGAGGCCT TCGAGGAGAT GGAGGGAACC 
TCCCCGAGCA GCCCACCACC CAGTGGGGTG CGGTCCCCCC CGGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCTCACAAC CCAGCGGACA TCCTGTTGCA CCCCACAGGA 
GTTACCAGAA GACGGATCCA GCCAGATTCT CTTTCTCAAG AAATAATCCC TGTTAATCTC 
TTTCTCTCTT TTTCCTTTTT TGCTCTTCCT CATCTTCACC ACCTGCCCTT CTTTCCTGCC 
TCTCCTTCCT CCCACCTCTC TTCTCGTCTT CCCTCAGAGG AAGATGAGGG GAAGGTGGTG 
GTCAGGCTGT CAGAAGAGCC CCGGAGCTAT GTGGAGTCTG TGGCACGGAC AGCGGTGGCT 
GGACCCCGAG CTCAGGACTC TGAGCCCAAG AGCTTTAGTG CTCCAGCCAC CCAGGCCTAT 
GGCCATGAGA TACCCCTGAG GAACGGGACC CTGGGTGGCT CCTTTGTCTC CCCCAGCCCC 
CTCTCCACCA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CAGTGGGGAG TTTCCCGTCG 
GGAGAGAGCA GTGACCAGGG TCCCCGGACG CCCACCCAGC CTCTGTTGGA GTCTGGCTTC 
CGCTCAGGCA GCCTGGGACA GCCCAGCCCG TCTGCCCAGA GAAACTACCA GAGCTCTTCT 
CCTCTCCCGA CTGTGGGCAG TAGCTACAGC AGCCCCGACT ACTCACTTCA GCATTTCAGC 
TCCTCTCCGG AAAGCCAGGC TCGAGCTCAG TTCAGTGTGG CTGGCGTCCA CACGGTGCCT 
GGGAGCCCTC AGGCGCGCCA CAGAACAGTG GGCACCAACA CTCCCCCTAG TCCTGGCTTC 
GGCTGGCGGG CCATCAATCC CAGCATGGCT GCCCCCAGCA GTCCCAGTTT GAGCCATCAC 
CAGATGATGG GTCCACCAGG CACTGGCTTC CATGGTAGCA CTGTCTCCAG CCCCCAGAGC 
AGTGCAGCGA CCACCCCGGG GAGCCCCAGC CTGTGTCGGC ACCCAGCAGG GGTCTACCAG 
GTTTCTGGCC TCCACAACAA AGTGGCCACC ACCCCGGGGA GTCCCAGCCT GGGCCGGCAC 
CCTGGGGCTC ACCAAGGCAA CCTGGCCTCC GGTCTTCATA GCAATGCAAT AGCCAGCCCT 
GGAAGCCCCA GCCTGGGCCG TCACCTCGGA GGGTCTGGAT CTGTGGTTCC CGGCAGCCCC 
TGCTTGGACC GGCATGTGGC CTATGGTGGC TATTCTACCC CGGAGGATCG GAGACCCACA 
CTGTCCCGGC AGAGCAGTGC CTCTGGCTAC CAGGCTCCTT CCACGCCCTC CTTCCCTGTC 
TCCCCTGCCT ACTACCCTGG CCTGAGCAGC CCTGCCACCT CCCCGTCACC AGACTCCGCA 
GCCTTCCGGC AAGGGAGCCC AACACCAGCC TTGCCAGAGA AGCGAAGGAT GTCAGTGGGA 
GACCGGGCAG GCAGCCTCCC CAACTATGCC ACCATCAATG GGAAGGTGTC TTCGCCTGTC 
GCCAGCGGCA TGTCCAGTCC CAGTGGGGGC AGCACCGTCT CCTTCTCCCA CACTCTGCCC 
GACTTCTCCA AGTACTCCAT GCCAGACAAC AGCCCGGAGA CGCGGGCTAA AGTGAAGTTT 
GTCCAGGACA CTTCTAAGTA TTGGTACAAG CCTGAGATCT CCAGGGAGCA GGCCATCGCG 
CTCCTCAAGG ACCAGGAGCC GGGGGCCTTC ATCATCCGCG ACAGTCACTC CTTCCGAGGC 
GCGTACGGGC TGGCCATGAA GGTGTCTTCG CCACCTCCAA CCATCATGCA GCAGAATAAA 
AAAGGAGACA TGACCCATGA GCTGGTCAGG CATTTTCTGA TAGAGACTGG CCCCAGAGGA 
GTCAAGCTCA AGGGCTGCCC CAATGAGCCA AACTTCGGAT CGCTGTCTGC CCTGGTCTAC 
CAGCACTCCA TCATCCCATT GGCCCTGCCT TGCAAGCTGG TCATTCCAAA CCGAGACCCC 
ACAGATGAAT CGAAAGATAG CTCCGGCCCT GCCAACTCAA CTGCAGACCT GCTGAAACAA 
GGGGCAGCCT GCAATGTGCT CTTCGTCAAC TCTGTGGACA TGGAGTCACT CACTGGGCCA 
CAGGCCATCT CTAAAGCCAC ATCTGAGACG TTGGCTGCAG ACCCCACGCC AGCTGCCACC 
ATCGTTCACT TCAAAGTCTC TGCCCAGGGA ATCACTCTGA CTGACAACCA GAGAAAGCTC 
TTTTTCAGAC GCCACTACCC TCTCAACACT GTCACCTTCT GTGACCTGGA TCCACAGGAA 
AGAAAGTGGA TGAAAACAGA GGGTGGTGCC CCTGCTAAGC TCTTCGGCTT CGTGGCCCGG 
AAGCAGGGCA GCACCACGGA CAACGCCTGC CACCTCTTTG CTGAGCTTGA CCCCAACCAG 
CCGGCCTCTG CCATCGTCAA CTTCGTCTCC AAGGTCATGC TGAATGCCGG CCAAAAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004803.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860292.1 Homo sapiens tensin 1 (TNS1), transcript variant X3, mRNA.

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CloneID	OHu89836
Clone ID Related Accession (Same CDS sequence)	XM_017004803.1
Accession Version	XM_017004803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGGGGT ACAGGCTCGG GAGAAGCAGC CTGCAGAGCC CCCAGCCCCT 
CTGCGGAGGC GGGCGGCCAG TGATGGACAG TATGAGAACC AGTCTCCAGA AGCCACATCC 
CCTCGTAGCC CTGGGGTTCG CTCCCCTGTC CAGTGTGTCT CCCCGGAGCT GGCTCTTACC 
ATCGCTCTCA ATCCTGGAGG GCGGCCCAAA GAGCCCCATT TGCACAGCTA CAAGGAGGCC 
TTCGAGGAGA TGGAGGGAAC CTCCCCGAGC AGCCCACCAC CCAGTGGGGT GCGGTCCCCC 
CCGGGTCTGG CCAAGACACC CCTGTCTGCT CTGGGCCTGA AACCTCACAA CCCAGCGGAC 
ATCCTGTTGC ACCCCACAGG AGTTACCAGA AGACGGATCC AGCCAGATTC TCTTTCTCAA 
GAAATAATCC CTGTTAATCT CTTTCTCTCT TTTTCCTTTT TTGCTCTTCC TCATCTTCAC 
CACCTGCCCT TCTTTCCTGC CTCTCCTTCC TCCCACCTCT CTTCTCGTCT TCCCTCAGAG 
GAAGATGAGG GGAAGGTGGT GGTCAGGCTG TCAGAAGAGC CCCGGAGCTA TGTGGAGTCT 
GTGGCACGGA CAGCGGTGGC TGGACCCCGA GCTCAGGACT CTGAGCCCAA GAGCTTTAGT 
GCTCCAGCCA CCCAGGCCTA TGGCCATGAG ATACCCCTGA GGAACGGGAC CCTGGGTGGC 
TCCTTTGTCT CCCCCAGCCC CCTCTCCACC AGCAGCCCCA TCCTCAGTGC TGACAGCACT 
TCAGTGGGGA GTTTCCCGTC GGGAGAGAGC AGTGACCAGG GTCCCCGGAC GCCCACCCAG 
CCTCTGTTGG AGTCTGGCTT CCGCTCAGGC AGCCTGGGAC AGCCCAGCCC GTCTGCCCAG 
AGAAACTACC AGAGCTCTTC TCCTCTCCCG ACTGTGGGCA GTAGCTACAG CAGCCCCGAC 
TACTCACTTC AGCATTTCAG CTCCTCTCCG GAAAGCCAGG CTCGAGCTCA GTTCAGTGTG 
GCTGGCGTCC ACACGGTGCC TGGGAGCCCT CAGGCGCGCC ACAGAACAGT GGGCACCAAC 
ACTCCCCCTA GTCCTGGCTT CGGCTGGCGG GCCATCAATC CCAGCATGGC TGCCCCCAGC 
AGTCCCAGTT TGAGCCATCA CCAGATGATG GGTCCACCAG GCACTGGCTT CCATGGTAGC 
ACTGTCTCCA GCCCCCAGAG CAGTGCAGCG ACCACCCCGG GGAGCCCCAG CCTGTGTCGG 
CACCCAGCAG GGGTCTACCA GGTTTCTGGC CTCCACAACA AAGTGGCCAC CACCCCGGGG 
AGTCCCAGCC TGGGCCGGCA CCCTGGGGCT CACCAAGGCA ACCTGGCCTC CGGTCTTCAT 
AGCAATGCAA TAGCCAGCCC TGGAAGCCCC AGCCTGGGCC GTCACCTCGG AGGGTCTGGA 
TCTGTGGTTC CCGGCAGCCC CTGCTTGGAC CGGCATGTGG CCTATGGTGG CTATTCTACC 
CCGGAGGATC GGAGACCCAC ACTGTCCCGG CAGAGCAGTG CCTCTGGCTA CCAGGCTCCT 
TCCACGCCCT CCTTCCCTGT CTCCCCTGCC TACTACCCTG GCCTGAGCAG CCCTGCCACC 
TCCCCGTCAC CAGACTCCGC AGCCTTCCGG CAAGGGAGCC CAACACCAGC CTTGCCAGAG 
AAGCGAAGGA TGTCAGTGGG AGACCGGGCA GGCAGCCTCC CCAACTATGC CACCATCAAT 
GGGAAGGTGT CTTCGCCTGT CGCCAGCGGC ATGTCCAGTC CCAGTGGGGG CAGCACCGTC 
TCCTTCTCCC ACACTCTGCC CGACTTCTCC AAGTACTCCA TGCCAGACAA CAGCCCGGAG 
ACGCGGGCTA AAGTGAAGTT TGTCCAGGAC ACTTCTAAGT ATTGGTACAA GCCTGAGATC 
TCCAGGGAGC AGGCCATCGC GCTCCTCAAG GACCAGGAGC CGGGGGCCTT CATCATCCGC 
GACAGTCACT CCTTCCGAGG CGCGTACGGG CTGGCCATGA AGGTGTCTTC GCCACCTCCA 
ACCATCATGC AGCAGAATAA AAAAGGAGAC ATGACCCATG AGCTGGTCAG GCATTTTCTG 
ATAGAGACTG GCCCCAGAGG AGTCAAGCTC AAGGGCTGCC CCAATGAGCC AAACTTCGGA 
TCGCTGTCTG CCCTGGTCTA CCAGCACTCC ATCATCCCAT TGGCCCTGCC TTGCAAGCTG 
GTCATTCCAA ACCGAGACCC CACAGATGAA TCGAAAGATA GCTCCGGCCC TGCCAACTCA 
ACTGCAGACC TGCTGAAACA AGGGGCAGCC TGCAATGTGC TCTTCGTCAA CTCTGTGGAC 
ATGGAGTCAC TCACTGGGCC ACAGGCCATC TCTAAAGCCA CATCTGAGAC GTTGGCTGCA 
GACCCCACGC CAGCTGCCAC CATCGTTCAC TTCAAAGTCT CTGCCCAGGG AATCACTCTG 
ACTGACAACC AGAGAAAGCT CTTTTTCAGA CGCCACTACC CTCTCAACAC TGTCACCTTC 
TGTGACCTGG ATCCACAGGA AAGAAAGTGG ATGAAAACAG AGGGTGGTGC CCCTGCTAAG 
CTCTTCGGCT TCGTGGCCCG GAAGCAGGGC AGCACCACGG ACAACGCCTG CCACCTCTTT 
GCTGAGCTTG ACCCCAACCA GCCGGCCTCT GCCATCGTCA ACTTCGTCTC CAAGGTCATG 
CTGAATGCCG GCCAAAAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860292.1
CDS	55..5658
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCINGNKQNTNSQSIGSISEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004803.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004803.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGGGGT ACAGGCTCGG GAGAAGCAGC CTGCAGAGCC CCCAGCCCCT 
CTGCGGAGGC GGGCGGCCAG TGATGGACAG TATGAGAACC AGTCTCCAGA AGCCACATCC 
CCTCGTAGCC CTGGGGTTCG CTCCCCTGTC CAGTGTGTCT CCCCGGAGCT GGCTCTTACC 
ATCGCTCTCA ATCCTGGAGG GCGGCCCAAA GAGCCCCATT TGCACAGCTA CAAGGAGGCC 
TTCGAGGAGA TGGAGGGAAC CTCCCCGAGC AGCCCACCAC CCAGTGGGGT GCGGTCCCCC 
CCGGGTCTGG CCAAGACACC CCTGTCTGCT CTGGGCCTGA AACCTCACAA CCCAGCGGAC 
ATCCTGTTGC ACCCCACAGG AGTTACCAGA AGACGGATCC AGCCAGATTC TCTTTCTCAA 
GAAATAATCC CTGTTAATCT CTTTCTCTCT TTTTCCTTTT TTGCTCTTCC TCATCTTCAC 
CACCTGCCCT TCTTTCCTGC CTCTCCTTCC TCCCACCTCT CTTCTCGTCT TCCCTCAGAG 
GAAGATGAGG GGAAGGTGGT GGTCAGGCTG TCAGAAGAGC CCCGGAGCTA TGTGGAGTCT 
GTGGCACGGA CAGCGGTGGC TGGACCCCGA GCTCAGGACT CTGAGCCCAA GAGCTTTAGT 
GCTCCAGCCA CCCAGGCCTA TGGCCATGAG ATACCCCTGA GGAACGGGAC CCTGGGTGGC 
TCCTTTGTCT CCCCCAGCCC CCTCTCCACC AGCAGCCCCA TCCTCAGTGC TGACAGCACT 
TCAGTGGGGA GTTTCCCGTC GGGAGAGAGC AGTGACCAGG GTCCCCGGAC GCCCACCCAG 
CCTCTGTTGG AGTCTGGCTT CCGCTCAGGC AGCCTGGGAC AGCCCAGCCC GTCTGCCCAG 
AGAAACTACC AGAGCTCTTC TCCTCTCCCG ACTGTGGGCA GTAGCTACAG CAGCCCCGAC 
TACTCACTTC AGCATTTCAG CTCCTCTCCG GAAAGCCAGG CTCGAGCTCA GTTCAGTGTG 
GCTGGCGTCC ACACGGTGCC TGGGAGCCCT CAGGCGCGCC ACAGAACAGT GGGCACCAAC 
ACTCCCCCTA GTCCTGGCTT CGGCTGGCGG GCCATCAATC CCAGCATGGC TGCCCCCAGC 
AGTCCCAGTT TGAGCCATCA CCAGATGATG GGTCCACCAG GCACTGGCTT CCATGGTAGC 
ACTGTCTCCA GCCCCCAGAG CAGTGCAGCG ACCACCCCGG GGAGCCCCAG CCTGTGTCGG 
CACCCAGCAG GGGTCTACCA GGTTTCTGGC CTCCACAACA AAGTGGCCAC CACCCCGGGG 
AGTCCCAGCC TGGGCCGGCA CCCTGGGGCT CACCAAGGCA ACCTGGCCTC CGGTCTTCAT 
AGCAATGCAA TAGCCAGCCC TGGAAGCCCC AGCCTGGGCC GTCACCTCGG AGGGTCTGGA 
TCTGTGGTTC CCGGCAGCCC CTGCTTGGAC CGGCATGTGG CCTATGGTGG CTATTCTACC 
CCGGAGGATC GGAGACCCAC ACTGTCCCGG CAGAGCAGTG CCTCTGGCTA CCAGGCTCCT 
TCCACGCCCT CCTTCCCTGT CTCCCCTGCC TACTACCCTG GCCTGAGCAG CCCTGCCACC 
TCCCCGTCAC CAGACTCCGC AGCCTTCCGG CAAGGGAGCC CAACACCAGC CTTGCCAGAG 
AAGCGAAGGA TGTCAGTGGG AGACCGGGCA GGCAGCCTCC CCAACTATGC CACCATCAAT 
GGGAAGGTGT CTTCGCCTGT CGCCAGCGGC ATGTCCAGTC CCAGTGGGGG CAGCACCGTC 
TCCTTCTCCC ACACTCTGCC CGACTTCTCC AAGTACTCCA TGCCAGACAA CAGCCCGGAG 
ACGCGGGCTA AAGTGAAGTT TGTCCAGGAC ACTTCTAAGT ATTGGTACAA GCCTGAGATC 
TCCAGGGAGC AGGCCATCGC GCTCCTCAAG GACCAGGAGC CGGGGGCCTT CATCATCCGC 
GACAGTCACT CCTTCCGAGG CGCGTACGGG CTGGCCATGA AGGTGTCTTC GCCACCTCCA 
ACCATCATGC AGCAGAATAA AAAAGGAGAC ATGACCCATG AGCTGGTCAG GCATTTTCTG 
ATAGAGACTG GCCCCAGAGG AGTCAAGCTC AAGGGCTGCC CCAATGAGCC AAACTTCGGA 
TCGCTGTCTG CCCTGGTCTA CCAGCACTCC ATCATCCCAT TGGCCCTGCC TTGCAAGCTG 
GTCATTCCAA ACCGAGACCC CACAGATGAA TCGAAAGATA GCTCCGGCCC TGCCAACTCA 
ACTGCAGACC TGCTGAAACA AGGGGCAGCC TGCAATGTGC TCTTCGTCAA CTCTGTGGAC 
ATGGAGTCAC TCACTGGGCC ACAGGCCATC TCTAAAGCCA CATCTGAGAC GTTGGCTGCA 
GACCCCACGC CAGCTGCCAC CATCGTTCAC TTCAAAGTCT CTGCCCAGGG AATCACTCTG 
ACTGACAACC AGAGAAAGCT CTTTTTCAGA CGCCACTACC CTCTCAACAC TGTCACCTTC 
TGTGACCTGG ATCCACAGGA AAGAAAGTGG ATGAAAACAG AGGGTGGTGC CCCTGCTAAG 
CTCTTCGGCT TCGTGGCCCG GAAGCAGGGC AGCACCACGG ACAACGCCTG CCACCTCTTT 
GCTGAGCTTG ACCCCAACCA GCCGGCCTCT GCCATCGTCA ACTTCGTCTC CAAGGTCATG 
CTGAATGCCG GCCAAAAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004805.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860294.1 Homo sapiens tensin 1 (TNS1), transcript variant X8, mRNA.

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CloneID	OHu89837
Clone ID Related Accession (Same CDS sequence)	XM_017004805.1 , XM_024453072.1
Accession Version	XM_017004805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGATGGGA ACAAACAGAA TACCAATAGC 
CAGTCAATTG GAAGCATCTC AGAGGTGGTG GGACACACGC AGGGGCCACT AGATGGGAGC 
CTGTATGCTA AGGTGAAGAA GAAAGACTCC CTGCACGGCA GCACCGGGGC TGTTAATGCC 
ACACGTCCTA CACTGTCGGC CACCCCCAAC CACGTGGAAC ACACGCTTTC TGTGAGCAGC 
GACTCGGGCA ACTCCACAGC CTCCACCAAG ACCGACAAGA CCGACGAGCC TGTCCCCGGG 
GCCTCCAGTG CCACTGCTGC CTTGAGTCCC CAGGAGAAGC GGGAGCTGGA CCGCCTGCTG 
AGTGGCTTTG GCTTAGAGCG AGAGAAGCAA GGCGCCATGT ACCACACCCA GCACCTCAGG 
TCCCGCCCAG CAGGGGGCTC GGCTGTGCCC TCCTCTGGAC GCCACGTTGT CCCAGCCCAG 
GTTCATGTCA ATGGTGGGGC GTTAGCATCT GAGCGGGAGA CAGACATCCT GGACGATGAA 
TTGCCAAACC AGGATGGTCA CAGTGCGGGC AGCATGGGCA CACTCTCTTC TCTGGACGGG 
GTCACCAACA CCAGTGAGGG GGGCTACCCA GAGGCCCTGT CCCCACTGAC CAACGGTCTG 
GACAAGTCCT ACCCCATGGA GCCTATGGTC AATGGAGGAG GCTACCCCTA CGAGTCTGCC 
AGCCGGGCGG GGCCTGCCCA TGCTGGCCAC ACGGCCCCCA TGCGGCCCTC CTACTCTGCA 
CAGGAGGGTT TAGCTGGCTA CCAGAGGGAG GGGCCCCACC CAGCCTGGCC ACAGCCAGTG 
ACCACCTCCC ACTATGCCCA TGACCCCAGC GGTATGTTCC GCTCTCAATC CTTTTCGGAA 
GCTGAACCCC AGCTGCCCCC AGCTCCGGTC CGAGGGGGAA GCAGCCGGGA GGCTGTGCAA 
AGGGGACTGA ATTCGTGGCA GCAGCAGCAG CAGCAGCAGC AGCAGCCTCG CCCACCTCCA 
CGCCAGCAGG AAAGAGCCCA CTTGGAGAGT CTTGTAGCCA GCAGGCCCAG CCCTCAGCCA 
TTGGCAGAGA CCCCCATCCC CAGTCTCCCT GAGTTCCCGC GAGCAGCCTC CCAGCAGGAG 
ATTGAACAGT CCATCGAAAC ACTCAATATG CTGATGCTGG ACCTGGAGCC AGCCTCCGCT 
GCTGCCCCAC TACACAAGTC CCAGAGTGTC CCCGGGGCCT GGCCAGGGGC TTCTCCACTC 
TCCTCCCAGC CCCTCTCTGG ATCCTCCCGT CAGTCCCATC CACTGACCCA GTCCAGATCT 
GGCTATATCC CCAGTGGGCA TTCGTTGGGA ACCCCTGAGC CAGCCCCACG GGCCTCTCTG 
GAGTCTGTCC CTCCTGGCAG GTCTTACTCA CCTTATGACT ATCAGCCATG TTTGGCTGGG 
CCTAACCAGG ATTTCCATTC AAAGAGCCCA GCCTCTTCCT CCTTGCCTGC CTTCCTTCCG 
ACCACCCACA GCCCTCCAGG GCCTCAGCAA CCCCCAGCCT CTCTCCCTGG CCTCACTGCT 
CAGCCTCTGC TCTCACCAAA GGAAGCGACT TCAGACCCCT CCCGGACTCC AGAGGAGGAG 
CCATTGAATT TAGAAGGGCT GGTGGCCCAC AGGGTAGCAG CGTACAACGC CAGGCTGCAG 
GGCATCGGAC ACAGTGGCAG CTTCCCGCCC CCTCCTCTCC ACCGGCTCCA ATCTTCCTCC 
CTCTCTGGGG TACAGGCTCG GGAGAAGCAG CCTGCAGAGC CCCCAGCCCC TCTGCGGAGG 
CGGGCGGCCA GTGATGGACA GTATGAGAAC CAGTCTCCAG AAGCCACATC CCCTCGTAGC 
CCTGGGGTTC GCTCCCCTGT CCAGTGTGTC TCCCCGGAGC TGGCTCTTAC CATCGCTCTC 
AATCCTGGAG GGCGGCCCAA AGAGCCCCAT TTGCACAGCT ACAAGGAGGC CTTCGAGGAG 
ATGGAGGGAA CCTCCCCGAG CAGCCCACCA CCCAGTGGGG TGCGGTCCCC CCCGGGTCTG 
GCCAAGACAC CCCTGTCTGC TCTGGGCCTG AAACCTCACA ACCCAGCGGA CATCCTGTTG 
CACCCCACAG GAGTTACCAG AAGACGGATC CAGCCAGATT CTCTTTCTCA AGAAATAATC 
CCTGTTAATC TCTTTCTCTC TTTTTCCTTT TTTGCTCTTC CTCATCTTCA CCACCTGCCC 
TTCTTTCCTG CCTCTCCTTC CTCCCACCTC TCTTCTCGTC TTCCCTCAGA GGAAGATGAG 
GGGAAGGTGG TGGTCAGGCT GTCAGAAGAG CCCCGGAGCT ATGTGGAGTC TGTGGCACGG 
ACAGCGGTGG CTGGACCCCG AGCTCAGGAC TCTGAGCCCA AGAGCTTTAG TGCTCCAGCC 
ACCCAGGCCT ATGGCCATGA GATACCCCTG AGGAACGGGA CCCTGGGTGG CTCCTTTGTC 
TCCCCCAGCC CCCTCTCCAC CAGCAGCCCC ATCCTCAGTG CTGACAGCAC TTCAGTGGGG 
AGTTTCCCGT CGGGAGAGAG CAGTGACCAG GGTCCCCGGA CGCCCACCCA GCCTCTGTTG 
GAGTCTGGCT TCCGCTCAGG CAGCCTGGGA CAGCCCAGCC CGTCTGCCCA GAGAAACTAC 
CAGAGCTCTT CTCCTCTCCC GACTGTGGGC AGTAGCTACA GCAGCCCCGA CTACTCACTT 
CAGCATTTCA GCTCCTCTCC GGAAAGCCAG GCTCGAGCTC AGTTCAGTGT GGCTGGCGTC 
CACACGGTGC CTGGGAGCCC TCAGGCGCGC CACAGAACAG TGGGCACCAA CACTCCCCCT 
AGTCCTGGCT TCGGCTGGCG GGCCATCAAT CCCAGCATGG CTGCCCCCAG CAGTCCCAGT 
TTGAGCCATC ACCAGATGAT GGGTCCACCA GGCACTGGCT TCCATGGTAG CACTGTCTCC 
AGCCCCCAGA GCAGTGCAGC GACCACCCCG GGGAGCCCCA GCCTGTGTCG GCACCCAGCA 
GGGGTCTACC AGGTTTCTGG CCTCCACAAC AAAGTGGCCA CCACCCCGGG GAGTCCCAGC 
CTGGGCCGGC ACCCTGGGGC TCACCAAGGC AACCTGGCCT CCGGTCTTCA TAGCAATGCA 
ATAGCCAGCC CTGGAAGCCC CAGCCTGGGC CGTCACCTCG GAGGGTCTGG ATCTGTGGTT 
CCCGGCAGCC CCTGCTTGGA CCGGCATGTG GCCTATGGTG GCTATTCTAC CCCGGAGGAT 
CGGAGACCCA CACTGTCCCG GCAGAGCAGT GCCTCTGGCT ACCAGGCTCC TTCCACGCCC 
TCCTTCCCTG TCTCCCCTGC CTACTACCCT GGCCTGAGCA GCCCTGCCAC CTCCCCGTCA 
CCAGACTCCG CAGCCTTCCG GCAAGGGAGC CCAACACCAG CCTTGCCAGA GAAGCGAAGG 
ATGTCAGTGG GAGACCGGGC AGGCAGCCTC CCCAACTATG CCACCATCAA TGGGAAGGTG 
TCTTCGCCTG TCGCCAGCGG CATGTCCAGT CCCAGTGGGG GCAGCACCGT CTCCTTCTCC 
CACACTCTGC CCGACTTCTC CAAGTACTCC ATGCCAGACA ACAGCCCGGA GACGCGGGCT 
AAAGTGAAGT TTGTCCAGGA CACTTCTAAG TATTGGTACA AGCCTGAGAT CTCCAGGGAG 
CAGGCCATCG CGCTCCTCAA GGACCAGGAG CCGGGGGCCT TCATCATCCG CGACAGTCAC 
TCCTTCCGAG GCGCGTACGG GCTGGCCATG AAGGTGTCTT CGCCACCTCC AACCATCATG 
CAGCAGAATA AAAAAGGAGA CATGACCCAT GAGCTGGTCA GGCATTTTCT GATAGAGACT 
GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC CCCAATGAGC CAAACTTCGG ATCGCTGTCT 
GCCCTGGTCT ACCAGCACTC CATCATCCCA TTGGCCCTGC CTTGCAAGCT GGTCATTCCA 
AACCGAGACC CCACAGATGA ATCGAAAGAT AGCTCCGGCC CTGCCAACTC AACTGCAGAC 
CTGCTGAAAC AAGGGGCAGC CTGCAATGTG CTCTTCGTCA ACTCTGTGGA CATGGAGTCA 
CTCACTGGGC CACAGGCCAT CTCTAAAGCC ACATCTGAGA CGTTGGCTGC AGACCCCACG 
CCAGCTGCCA CCATCGTTCA CTTCAAAGTC TCTGCCCAGG GAATCACTCT GACTGACAAC 
CAGAGAAAGC TCTTTTTCAG ACGCCACTAC CCTCTCAACA CTGTCACCTT CTGTGACCTG 
GATCCACAGG AAAGAAAGTG GATGAAAACA GAGGGTGGTG CCCCTGCTAA GCTCTTCGGC 
TTCGTGGCCC GGAAGCAGGG CAGCACCACG GACAACGCCT GCCACCTCTT TGCTGAGCTT 
GACCCCAACC AGCCGGCCTC TGCCATCGTC AACTTCGTCT CCAAGGTCAT GCTGAATGCC 
GGCCAAAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860294.1
CDS	55..5529
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEDGNKQNTNSQSIGSISEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004805.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004805.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGATGGGA ACAAACAGAA TACCAATAGC 
CAGTCAATTG GAAGCATCTC AGAGGTGGTG GGACACACGC AGGGGCCACT AGATGGGAGC 
CTGTATGCTA AGGTGAAGAA GAAAGACTCC CTGCACGGCA GCACCGGGGC TGTTAATGCC 
ACACGTCCTA CACTGTCGGC CACCCCCAAC CACGTGGAAC ACACGCTTTC TGTGAGCAGC 
GACTCGGGCA ACTCCACAGC CTCCACCAAG ACCGACAAGA CCGACGAGCC TGTCCCCGGG 
GCCTCCAGTG CCACTGCTGC CTTGAGTCCC CAGGAGAAGC GGGAGCTGGA CCGCCTGCTG 
AGTGGCTTTG GCTTAGAGCG AGAGAAGCAA GGCGCCATGT ACCACACCCA GCACCTCAGG 
TCCCGCCCAG CAGGGGGCTC GGCTGTGCCC TCCTCTGGAC GCCACGTTGT CCCAGCCCAG 
GTTCATGTCA ATGGTGGGGC GTTAGCATCT GAGCGGGAGA CAGACATCCT GGACGATGAA 
TTGCCAAACC AGGATGGTCA CAGTGCGGGC AGCATGGGCA CACTCTCTTC TCTGGACGGG 
GTCACCAACA CCAGTGAGGG GGGCTACCCA GAGGCCCTGT CCCCACTGAC CAACGGTCTG 
GACAAGTCCT ACCCCATGGA GCCTATGGTC AATGGAGGAG GCTACCCCTA CGAGTCTGCC 
AGCCGGGCGG GGCCTGCCCA TGCTGGCCAC ACGGCCCCCA TGCGGCCCTC CTACTCTGCA 
CAGGAGGGTT TAGCTGGCTA CCAGAGGGAG GGGCCCCACC CAGCCTGGCC ACAGCCAGTG 
ACCACCTCCC ACTATGCCCA TGACCCCAGC GGTATGTTCC GCTCTCAATC CTTTTCGGAA 
GCTGAACCCC AGCTGCCCCC AGCTCCGGTC CGAGGGGGAA GCAGCCGGGA GGCTGTGCAA 
AGGGGACTGA ATTCGTGGCA GCAGCAGCAG CAGCAGCAGC AGCAGCCTCG CCCACCTCCA 
CGCCAGCAGG AAAGAGCCCA CTTGGAGAGT CTTGTAGCCA GCAGGCCCAG CCCTCAGCCA 
TTGGCAGAGA CCCCCATCCC CAGTCTCCCT GAGTTCCCGC GAGCAGCCTC CCAGCAGGAG 
ATTGAACAGT CCATCGAAAC ACTCAATATG CTGATGCTGG ACCTGGAGCC AGCCTCCGCT 
GCTGCCCCAC TACACAAGTC CCAGAGTGTC CCCGGGGCCT GGCCAGGGGC TTCTCCACTC 
TCCTCCCAGC CCCTCTCTGG ATCCTCCCGT CAGTCCCATC CACTGACCCA GTCCAGATCT 
GGCTATATCC CCAGTGGGCA TTCGTTGGGA ACCCCTGAGC CAGCCCCACG GGCCTCTCTG 
GAGTCTGTCC CTCCTGGCAG GTCTTACTCA CCTTATGACT ATCAGCCATG TTTGGCTGGG 
CCTAACCAGG ATTTCCATTC AAAGAGCCCA GCCTCTTCCT CCTTGCCTGC CTTCCTTCCG 
ACCACCCACA GCCCTCCAGG GCCTCAGCAA CCCCCAGCCT CTCTCCCTGG CCTCACTGCT 
CAGCCTCTGC TCTCACCAAA GGAAGCGACT TCAGACCCCT CCCGGACTCC AGAGGAGGAG 
CCATTGAATT TAGAAGGGCT GGTGGCCCAC AGGGTAGCAG CGTACAACGC CAGGCTGCAG 
GGCATCGGAC ACAGTGGCAG CTTCCCGCCC CCTCCTCTCC ACCGGCTCCA ATCTTCCTCC 
CTCTCTGGGG TACAGGCTCG GGAGAAGCAG CCTGCAGAGC CCCCAGCCCC TCTGCGGAGG 
CGGGCGGCCA GTGATGGACA GTATGAGAAC CAGTCTCCAG AAGCCACATC CCCTCGTAGC 
CCTGGGGTTC GCTCCCCTGT CCAGTGTGTC TCCCCGGAGC TGGCTCTTAC CATCGCTCTC 
AATCCTGGAG GGCGGCCCAA AGAGCCCCAT TTGCACAGCT ACAAGGAGGC CTTCGAGGAG 
ATGGAGGGAA CCTCCCCGAG CAGCCCACCA CCCAGTGGGG TGCGGTCCCC CCCGGGTCTG 
GCCAAGACAC CCCTGTCTGC TCTGGGCCTG AAACCTCACA ACCCAGCGGA CATCCTGTTG 
CACCCCACAG GAGTTACCAG AAGACGGATC CAGCCAGATT CTCTTTCTCA AGAAATAATC 
CCTGTTAATC TCTTTCTCTC TTTTTCCTTT TTTGCTCTTC CTCATCTTCA CCACCTGCCC 
TTCTTTCCTG CCTCTCCTTC CTCCCACCTC TCTTCTCGTC TTCCCTCAGA GGAAGATGAG 
GGGAAGGTGG TGGTCAGGCT GTCAGAAGAG CCCCGGAGCT ATGTGGAGTC TGTGGCACGG 
ACAGCGGTGG CTGGACCCCG AGCTCAGGAC TCTGAGCCCA AGAGCTTTAG TGCTCCAGCC 
ACCCAGGCCT ATGGCCATGA GATACCCCTG AGGAACGGGA CCCTGGGTGG CTCCTTTGTC 
TCCCCCAGCC CCCTCTCCAC CAGCAGCCCC ATCCTCAGTG CTGACAGCAC TTCAGTGGGG 
AGTTTCCCGT CGGGAGAGAG CAGTGACCAG GGTCCCCGGA CGCCCACCCA GCCTCTGTTG 
GAGTCTGGCT TCCGCTCAGG CAGCCTGGGA CAGCCCAGCC CGTCTGCCCA GAGAAACTAC 
CAGAGCTCTT CTCCTCTCCC GACTGTGGGC AGTAGCTACA GCAGCCCCGA CTACTCACTT 
CAGCATTTCA GCTCCTCTCC GGAAAGCCAG GCTCGAGCTC AGTTCAGTGT GGCTGGCGTC 
CACACGGTGC CTGGGAGCCC TCAGGCGCGC CACAGAACAG TGGGCACCAA CACTCCCCCT 
AGTCCTGGCT TCGGCTGGCG GGCCATCAAT CCCAGCATGG CTGCCCCCAG CAGTCCCAGT 
TTGAGCCATC ACCAGATGAT GGGTCCACCA GGCACTGGCT TCCATGGTAG CACTGTCTCC 
AGCCCCCAGA GCAGTGCAGC GACCACCCCG GGGAGCCCCA GCCTGTGTCG GCACCCAGCA 
GGGGTCTACC AGGTTTCTGG CCTCCACAAC AAAGTGGCCA CCACCCCGGG GAGTCCCAGC 
CTGGGCCGGC ACCCTGGGGC TCACCAAGGC AACCTGGCCT CCGGTCTTCA TAGCAATGCA 
ATAGCCAGCC CTGGAAGCCC CAGCCTGGGC CGTCACCTCG GAGGGTCTGG ATCTGTGGTT 
CCCGGCAGCC CCTGCTTGGA CCGGCATGTG GCCTATGGTG GCTATTCTAC CCCGGAGGAT 
CGGAGACCCA CACTGTCCCG GCAGAGCAGT GCCTCTGGCT ACCAGGCTCC TTCCACGCCC 
TCCTTCCCTG TCTCCCCTGC CTACTACCCT GGCCTGAGCA GCCCTGCCAC CTCCCCGTCA 
CCAGACTCCG CAGCCTTCCG GCAAGGGAGC CCAACACCAG CCTTGCCAGA GAAGCGAAGG 
ATGTCAGTGG GAGACCGGGC AGGCAGCCTC CCCAACTATG CCACCATCAA TGGGAAGGTG 
TCTTCGCCTG TCGCCAGCGG CATGTCCAGT CCCAGTGGGG GCAGCACCGT CTCCTTCTCC 
CACACTCTGC CCGACTTCTC CAAGTACTCC ATGCCAGACA ACAGCCCGGA GACGCGGGCT 
AAAGTGAAGT TTGTCCAGGA CACTTCTAAG TATTGGTACA AGCCTGAGAT CTCCAGGGAG 
CAGGCCATCG CGCTCCTCAA GGACCAGGAG CCGGGGGCCT TCATCATCCG CGACAGTCAC 
TCCTTCCGAG GCGCGTACGG GCTGGCCATG AAGGTGTCTT CGCCACCTCC AACCATCATG 
CAGCAGAATA AAAAAGGAGA CATGACCCAT GAGCTGGTCA GGCATTTTCT GATAGAGACT 
GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC CCCAATGAGC CAAACTTCGG ATCGCTGTCT 
GCCCTGGTCT ACCAGCACTC CATCATCCCA TTGGCCCTGC CTTGCAAGCT GGTCATTCCA 
AACCGAGACC CCACAGATGA ATCGAAAGAT AGCTCCGGCC CTGCCAACTC AACTGCAGAC 
CTGCTGAAAC AAGGGGCAGC CTGCAATGTG CTCTTCGTCA ACTCTGTGGA CATGGAGTCA 
CTCACTGGGC CACAGGCCAT CTCTAAAGCC ACATCTGAGA CGTTGGCTGC AGACCCCACG 
CCAGCTGCCA CCATCGTTCA CTTCAAAGTC TCTGCCCAGG GAATCACTCT GACTGACAAC 
CAGAGAAAGC TCTTTTTCAG ACGCCACTAC CCTCTCAACA CTGTCACCTT CTGTGACCTG 
GATCCACAGG AAAGAAAGTG GATGAAAACA GAGGGTGGTG CCCCTGCTAA GCTCTTCGGC 
TTCGTGGCCC GGAAGCAGGG CAGCACCACG GACAACGCCT GCCACCTCTT TGCTGAGCTT 
GACCCCAACC AGCCGGCCTC TGCCATCGTC AACTTCGTCT CCAAGGTCAT GCTGAATGCC 
GGCCAAAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453072.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308840.1 Homo sapiens tensin 1 (TNS1), transcript variant X5, mRNA.

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CloneID	OHu89837
Clone ID Related Accession (Same CDS sequence)	XM_017004805.1 , XM_024453072.1
Accession Version	XM_024453072.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGATGGGA ACAAACAGAA TACCAATAGC 
CAGTCAATTG GAAGCATCTC AGAGGTGGTG GGACACACGC AGGGGCCACT AGATGGGAGC 
CTGTATGCTA AGGTGAAGAA GAAAGACTCC CTGCACGGCA GCACCGGGGC TGTTAATGCC 
ACACGTCCTA CACTGTCGGC CACCCCCAAC CACGTGGAAC ACACGCTTTC TGTGAGCAGC 
GACTCGGGCA ACTCCACAGC CTCCACCAAG ACCGACAAGA CCGACGAGCC TGTCCCCGGG 
GCCTCCAGTG CCACTGCTGC CTTGAGTCCC CAGGAGAAGC GGGAGCTGGA CCGCCTGCTG 
AGTGGCTTTG GCTTAGAGCG AGAGAAGCAA GGCGCCATGT ACCACACCCA GCACCTCAGG 
TCCCGCCCAG CAGGGGGCTC GGCTGTGCCC TCCTCTGGAC GCCACGTTGT CCCAGCCCAG 
GTTCATGTCA ATGGTGGGGC GTTAGCATCT GAGCGGGAGA CAGACATCCT GGACGATGAA 
TTGCCAAACC AGGATGGTCA CAGTGCGGGC AGCATGGGCA CACTCTCTTC TCTGGACGGG 
GTCACCAACA CCAGTGAGGG GGGCTACCCA GAGGCCCTGT CCCCACTGAC CAACGGTCTG 
GACAAGTCCT ACCCCATGGA GCCTATGGTC AATGGAGGAG GCTACCCCTA CGAGTCTGCC 
AGCCGGGCGG GGCCTGCCCA TGCTGGCCAC ACGGCCCCCA TGCGGCCCTC CTACTCTGCA 
CAGGAGGGTT TAGCTGGCTA CCAGAGGGAG GGGCCCCACC CAGCCTGGCC ACAGCCAGTG 
ACCACCTCCC ACTATGCCCA TGACCCCAGC GGTATGTTCC GCTCTCAATC CTTTTCGGAA 
GCTGAACCCC AGCTGCCCCC AGCTCCGGTC CGAGGGGGAA GCAGCCGGGA GGCTGTGCAA 
AGGGGACTGA ATTCGTGGCA GCAGCAGCAG CAGCAGCAGC AGCAGCCTCG CCCACCTCCA 
CGCCAGCAGG AAAGAGCCCA CTTGGAGAGT CTTGTAGCCA GCAGGCCCAG CCCTCAGCCA 
TTGGCAGAGA CCCCCATCCC CAGTCTCCCT GAGTTCCCGC GAGCAGCCTC CCAGCAGGAG 
ATTGAACAGT CCATCGAAAC ACTCAATATG CTGATGCTGG ACCTGGAGCC AGCCTCCGCT 
GCTGCCCCAC TACACAAGTC CCAGAGTGTC CCCGGGGCCT GGCCAGGGGC TTCTCCACTC 
TCCTCCCAGC CCCTCTCTGG ATCCTCCCGT CAGTCCCATC CACTGACCCA GTCCAGATCT 
GGCTATATCC CCAGTGGGCA TTCGTTGGGA ACCCCTGAGC CAGCCCCACG GGCCTCTCTG 
GAGTCTGTCC CTCCTGGCAG GTCTTACTCA CCTTATGACT ATCAGCCATG TTTGGCTGGG 
CCTAACCAGG ATTTCCATTC AAAGAGCCCA GCCTCTTCCT CCTTGCCTGC CTTCCTTCCG 
ACCACCCACA GCCCTCCAGG GCCTCAGCAA CCCCCAGCCT CTCTCCCTGG CCTCACTGCT 
CAGCCTCTGC TCTCACCAAA GGAAGCGACT TCAGACCCCT CCCGGACTCC AGAGGAGGAG 
CCATTGAATT TAGAAGGGCT GGTGGCCCAC AGGGTAGCAG CGTACAACGC CAGGCTGCAG 
GGCATCGGAC ACAGTGGCAG CTTCCCGCCC CCTCCTCTCC ACCGGCTCCA ATCTTCCTCC 
CTCTCTGGGG TACAGGCTCG GGAGAAGCAG CCTGCAGAGC CCCCAGCCCC TCTGCGGAGG 
CGGGCGGCCA GTGATGGACA GTATGAGAAC CAGTCTCCAG AAGCCACATC CCCTCGTAGC 
CCTGGGGTTC GCTCCCCTGT CCAGTGTGTC TCCCCGGAGC TGGCTCTTAC CATCGCTCTC 
AATCCTGGAG GGCGGCCCAA AGAGCCCCAT TTGCACAGCT ACAAGGAGGC CTTCGAGGAG 
ATGGAGGGAA CCTCCCCGAG CAGCCCACCA CCCAGTGGGG TGCGGTCCCC CCCGGGTCTG 
GCCAAGACAC CCCTGTCTGC TCTGGGCCTG AAACCTCACA ACCCAGCGGA CATCCTGTTG 
CACCCCACAG GAGTTACCAG AAGACGGATC CAGCCAGATT CTCTTTCTCA AGAAATAATC 
CCTGTTAATC TCTTTCTCTC TTTTTCCTTT TTTGCTCTTC CTCATCTTCA CCACCTGCCC 
TTCTTTCCTG CCTCTCCTTC CTCCCACCTC TCTTCTCGTC TTCCCTCAGA GGAAGATGAG 
GGGAAGGTGG TGGTCAGGCT GTCAGAAGAG CCCCGGAGCT ATGTGGAGTC TGTGGCACGG 
ACAGCGGTGG CTGGACCCCG AGCTCAGGAC TCTGAGCCCA AGAGCTTTAG TGCTCCAGCC 
ACCCAGGCCT ATGGCCATGA GATACCCCTG AGGAACGGGA CCCTGGGTGG CTCCTTTGTC 
TCCCCCAGCC CCCTCTCCAC CAGCAGCCCC ATCCTCAGTG CTGACAGCAC TTCAGTGGGG 
AGTTTCCCGT CGGGAGAGAG CAGTGACCAG GGTCCCCGGA CGCCCACCCA GCCTCTGTTG 
GAGTCTGGCT TCCGCTCAGG CAGCCTGGGA CAGCCCAGCC CGTCTGCCCA GAGAAACTAC 
CAGAGCTCTT CTCCTCTCCC GACTGTGGGC AGTAGCTACA GCAGCCCCGA CTACTCACTT 
CAGCATTTCA GCTCCTCTCC GGAAAGCCAG GCTCGAGCTC AGTTCAGTGT GGCTGGCGTC 
CACACGGTGC CTGGGAGCCC TCAGGCGCGC CACAGAACAG TGGGCACCAA CACTCCCCCT 
AGTCCTGGCT TCGGCTGGCG GGCCATCAAT CCCAGCATGG CTGCCCCCAG CAGTCCCAGT 
TTGAGCCATC ACCAGATGAT GGGTCCACCA GGCACTGGCT TCCATGGTAG CACTGTCTCC 
AGCCCCCAGA GCAGTGCAGC GACCACCCCG GGGAGCCCCA GCCTGTGTCG GCACCCAGCA 
GGGGTCTACC AGGTTTCTGG CCTCCACAAC AAAGTGGCCA CCACCCCGGG GAGTCCCAGC 
CTGGGCCGGC ACCCTGGGGC TCACCAAGGC AACCTGGCCT CCGGTCTTCA TAGCAATGCA 
ATAGCCAGCC CTGGAAGCCC CAGCCTGGGC CGTCACCTCG GAGGGTCTGG ATCTGTGGTT 
CCCGGCAGCC CCTGCTTGGA CCGGCATGTG GCCTATGGTG GCTATTCTAC CCCGGAGGAT 
CGGAGACCCA CACTGTCCCG GCAGAGCAGT GCCTCTGGCT ACCAGGCTCC TTCCACGCCC 
TCCTTCCCTG TCTCCCCTGC CTACTACCCT GGCCTGAGCA GCCCTGCCAC CTCCCCGTCA 
CCAGACTCCG CAGCCTTCCG GCAAGGGAGC CCAACACCAG CCTTGCCAGA GAAGCGAAGG 
ATGTCAGTGG GAGACCGGGC AGGCAGCCTC CCCAACTATG CCACCATCAA TGGGAAGGTG 
TCTTCGCCTG TCGCCAGCGG CATGTCCAGT CCCAGTGGGG GCAGCACCGT CTCCTTCTCC 
CACACTCTGC CCGACTTCTC CAAGTACTCC ATGCCAGACA ACAGCCCGGA GACGCGGGCT 
AAAGTGAAGT TTGTCCAGGA CACTTCTAAG TATTGGTACA AGCCTGAGAT CTCCAGGGAG 
CAGGCCATCG CGCTCCTCAA GGACCAGGAG CCGGGGGCCT TCATCATCCG CGACAGTCAC 
TCCTTCCGAG GCGCGTACGG GCTGGCCATG AAGGTGTCTT CGCCACCTCC AACCATCATG 
CAGCAGAATA AAAAAGGAGA CATGACCCAT GAGCTGGTCA GGCATTTTCT GATAGAGACT 
GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC CCCAATGAGC CAAACTTCGG ATCGCTGTCT 
GCCCTGGTCT ACCAGCACTC CATCATCCCA TTGGCCCTGC CTTGCAAGCT GGTCATTCCA 
AACCGAGACC CCACAGATGA ATCGAAAGAT AGCTCCGGCC CTGCCAACTC AACTGCAGAC 
CTGCTGAAAC AAGGGGCAGC CTGCAATGTG CTCTTCGTCA ACTCTGTGGA CATGGAGTCA 
CTCACTGGGC CACAGGCCAT CTCTAAAGCC ACATCTGAGA CGTTGGCTGC AGACCCCACG 
CCAGCTGCCA CCATCGTTCA CTTCAAAGTC TCTGCCCAGG GAATCACTCT GACTGACAAC 
CAGAGAAAGC TCTTTTTCAG ACGCCACTAC CCTCTCAACA CTGTCACCTT CTGTGACCTG 
GATCCACAGG AAAGAAAGTG GATGAAAACA GAGGGTGGTG CCCCTGCTAA GCTCTTCGGC 
TTCGTGGCCC GGAAGCAGGG CAGCACCACG GACAACGCCT GCCACCTCTT TGCTGAGCTT 
GACCCCAACC AGCCGGCCTC TGCCATCGTC AACTTCGTCT CCAAGGTCAT GCTGAATGCC 
GGCCAAAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308840.1
CDS	190..5664
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEDGNKQNTNSQSIGSISEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453072.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453072.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGATGGGA ACAAACAGAA TACCAATAGC 
CAGTCAATTG GAAGCATCTC AGAGGTGGTG GGACACACGC AGGGGCCACT AGATGGGAGC 
CTGTATGCTA AGGTGAAGAA GAAAGACTCC CTGCACGGCA GCACCGGGGC TGTTAATGCC 
ACACGTCCTA CACTGTCGGC CACCCCCAAC CACGTGGAAC ACACGCTTTC TGTGAGCAGC 
GACTCGGGCA ACTCCACAGC CTCCACCAAG ACCGACAAGA CCGACGAGCC TGTCCCCGGG 
GCCTCCAGTG CCACTGCTGC CTTGAGTCCC CAGGAGAAGC GGGAGCTGGA CCGCCTGCTG 
AGTGGCTTTG GCTTAGAGCG AGAGAAGCAA GGCGCCATGT ACCACACCCA GCACCTCAGG 
TCCCGCCCAG CAGGGGGCTC GGCTGTGCCC TCCTCTGGAC GCCACGTTGT CCCAGCCCAG 
GTTCATGTCA ATGGTGGGGC GTTAGCATCT GAGCGGGAGA CAGACATCCT GGACGATGAA 
TTGCCAAACC AGGATGGTCA CAGTGCGGGC AGCATGGGCA CACTCTCTTC TCTGGACGGG 
GTCACCAACA CCAGTGAGGG GGGCTACCCA GAGGCCCTGT CCCCACTGAC CAACGGTCTG 
GACAAGTCCT ACCCCATGGA GCCTATGGTC AATGGAGGAG GCTACCCCTA CGAGTCTGCC 
AGCCGGGCGG GGCCTGCCCA TGCTGGCCAC ACGGCCCCCA TGCGGCCCTC CTACTCTGCA 
CAGGAGGGTT TAGCTGGCTA CCAGAGGGAG GGGCCCCACC CAGCCTGGCC ACAGCCAGTG 
ACCACCTCCC ACTATGCCCA TGACCCCAGC GGTATGTTCC GCTCTCAATC CTTTTCGGAA 
GCTGAACCCC AGCTGCCCCC AGCTCCGGTC CGAGGGGGAA GCAGCCGGGA GGCTGTGCAA 
AGGGGACTGA ATTCGTGGCA GCAGCAGCAG CAGCAGCAGC AGCAGCCTCG CCCACCTCCA 
CGCCAGCAGG AAAGAGCCCA CTTGGAGAGT CTTGTAGCCA GCAGGCCCAG CCCTCAGCCA 
TTGGCAGAGA CCCCCATCCC CAGTCTCCCT GAGTTCCCGC GAGCAGCCTC CCAGCAGGAG 
ATTGAACAGT CCATCGAAAC ACTCAATATG CTGATGCTGG ACCTGGAGCC AGCCTCCGCT 
GCTGCCCCAC TACACAAGTC CCAGAGTGTC CCCGGGGCCT GGCCAGGGGC TTCTCCACTC 
TCCTCCCAGC CCCTCTCTGG ATCCTCCCGT CAGTCCCATC CACTGACCCA GTCCAGATCT 
GGCTATATCC CCAGTGGGCA TTCGTTGGGA ACCCCTGAGC CAGCCCCACG GGCCTCTCTG 
GAGTCTGTCC CTCCTGGCAG GTCTTACTCA CCTTATGACT ATCAGCCATG TTTGGCTGGG 
CCTAACCAGG ATTTCCATTC AAAGAGCCCA GCCTCTTCCT CCTTGCCTGC CTTCCTTCCG 
ACCACCCACA GCCCTCCAGG GCCTCAGCAA CCCCCAGCCT CTCTCCCTGG CCTCACTGCT 
CAGCCTCTGC TCTCACCAAA GGAAGCGACT TCAGACCCCT CCCGGACTCC AGAGGAGGAG 
CCATTGAATT TAGAAGGGCT GGTGGCCCAC AGGGTAGCAG CGTACAACGC CAGGCTGCAG 
GGCATCGGAC ACAGTGGCAG CTTCCCGCCC CCTCCTCTCC ACCGGCTCCA ATCTTCCTCC 
CTCTCTGGGG TACAGGCTCG GGAGAAGCAG CCTGCAGAGC CCCCAGCCCC TCTGCGGAGG 
CGGGCGGCCA GTGATGGACA GTATGAGAAC CAGTCTCCAG AAGCCACATC CCCTCGTAGC 
CCTGGGGTTC GCTCCCCTGT CCAGTGTGTC TCCCCGGAGC TGGCTCTTAC CATCGCTCTC 
AATCCTGGAG GGCGGCCCAA AGAGCCCCAT TTGCACAGCT ACAAGGAGGC CTTCGAGGAG 
ATGGAGGGAA CCTCCCCGAG CAGCCCACCA CCCAGTGGGG TGCGGTCCCC CCCGGGTCTG 
GCCAAGACAC CCCTGTCTGC TCTGGGCCTG AAACCTCACA ACCCAGCGGA CATCCTGTTG 
CACCCCACAG GAGTTACCAG AAGACGGATC CAGCCAGATT CTCTTTCTCA AGAAATAATC 
CCTGTTAATC TCTTTCTCTC TTTTTCCTTT TTTGCTCTTC CTCATCTTCA CCACCTGCCC 
TTCTTTCCTG CCTCTCCTTC CTCCCACCTC TCTTCTCGTC TTCCCTCAGA GGAAGATGAG 
GGGAAGGTGG TGGTCAGGCT GTCAGAAGAG CCCCGGAGCT ATGTGGAGTC TGTGGCACGG 
ACAGCGGTGG CTGGACCCCG AGCTCAGGAC TCTGAGCCCA AGAGCTTTAG TGCTCCAGCC 
ACCCAGGCCT ATGGCCATGA GATACCCCTG AGGAACGGGA CCCTGGGTGG CTCCTTTGTC 
TCCCCCAGCC CCCTCTCCAC CAGCAGCCCC ATCCTCAGTG CTGACAGCAC TTCAGTGGGG 
AGTTTCCCGT CGGGAGAGAG CAGTGACCAG GGTCCCCGGA CGCCCACCCA GCCTCTGTTG 
GAGTCTGGCT TCCGCTCAGG CAGCCTGGGA CAGCCCAGCC CGTCTGCCCA GAGAAACTAC 
CAGAGCTCTT CTCCTCTCCC GACTGTGGGC AGTAGCTACA GCAGCCCCGA CTACTCACTT 
CAGCATTTCA GCTCCTCTCC GGAAAGCCAG GCTCGAGCTC AGTTCAGTGT GGCTGGCGTC 
CACACGGTGC CTGGGAGCCC TCAGGCGCGC CACAGAACAG TGGGCACCAA CACTCCCCCT 
AGTCCTGGCT TCGGCTGGCG GGCCATCAAT CCCAGCATGG CTGCCCCCAG CAGTCCCAGT 
TTGAGCCATC ACCAGATGAT GGGTCCACCA GGCACTGGCT TCCATGGTAG CACTGTCTCC 
AGCCCCCAGA GCAGTGCAGC GACCACCCCG GGGAGCCCCA GCCTGTGTCG GCACCCAGCA 
GGGGTCTACC AGGTTTCTGG CCTCCACAAC AAAGTGGCCA CCACCCCGGG GAGTCCCAGC 
CTGGGCCGGC ACCCTGGGGC TCACCAAGGC AACCTGGCCT CCGGTCTTCA TAGCAATGCA 
ATAGCCAGCC CTGGAAGCCC CAGCCTGGGC CGTCACCTCG GAGGGTCTGG ATCTGTGGTT 
CCCGGCAGCC CCTGCTTGGA CCGGCATGTG GCCTATGGTG GCTATTCTAC CCCGGAGGAT 
CGGAGACCCA CACTGTCCCG GCAGAGCAGT GCCTCTGGCT ACCAGGCTCC TTCCACGCCC 
TCCTTCCCTG TCTCCCCTGC CTACTACCCT GGCCTGAGCA GCCCTGCCAC CTCCCCGTCA 
CCAGACTCCG CAGCCTTCCG GCAAGGGAGC CCAACACCAG CCTTGCCAGA GAAGCGAAGG 
ATGTCAGTGG GAGACCGGGC AGGCAGCCTC CCCAACTATG CCACCATCAA TGGGAAGGTG 
TCTTCGCCTG TCGCCAGCGG CATGTCCAGT CCCAGTGGGG GCAGCACCGT CTCCTTCTCC 
CACACTCTGC CCGACTTCTC CAAGTACTCC ATGCCAGACA ACAGCCCGGA GACGCGGGCT 
AAAGTGAAGT TTGTCCAGGA CACTTCTAAG TATTGGTACA AGCCTGAGAT CTCCAGGGAG 
CAGGCCATCG CGCTCCTCAA GGACCAGGAG CCGGGGGCCT TCATCATCCG CGACAGTCAC 
TCCTTCCGAG GCGCGTACGG GCTGGCCATG AAGGTGTCTT CGCCACCTCC AACCATCATG 
CAGCAGAATA AAAAAGGAGA CATGACCCAT GAGCTGGTCA GGCATTTTCT GATAGAGACT 
GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC CCCAATGAGC CAAACTTCGG ATCGCTGTCT 
GCCCTGGTCT ACCAGCACTC CATCATCCCA TTGGCCCTGC CTTGCAAGCT GGTCATTCCA 
AACCGAGACC CCACAGATGA ATCGAAAGAT AGCTCCGGCC CTGCCAACTC AACTGCAGAC 
CTGCTGAAAC AAGGGGCAGC CTGCAATGTG CTCTTCGTCA ACTCTGTGGA CATGGAGTCA 
CTCACTGGGC CACAGGCCAT CTCTAAAGCC ACATCTGAGA CGTTGGCTGC AGACCCCACG 
CCAGCTGCCA CCATCGTTCA CTTCAAAGTC TCTGCCCAGG GAATCACTCT GACTGACAAC 
CAGAGAAAGC TCTTTTTCAG ACGCCACTAC CCTCTCAACA CTGTCACCTT CTGTGACCTG 
GATCCACAGG AAAGAAAGTG GATGAAAACA GAGGGTGGTG CCCCTGCTAA GCTCTTCGGC 
TTCGTGGCCC GGAAGCAGGG CAGCACCACG GACAACGCCT GCCACCTCTT TGCTGAGCTT 
GACCCCAACC AGCCGGCCTC TGCCATCGTC AACTTCGTCT CCAAGGTCAT GCTGAATGCC 
GGCCAAAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004806.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860295.1 Homo sapiens tensin 1 (TNS1), transcript variant X9, mRNA.

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CloneID	OHu89838
Clone ID Related Accession (Same CDS sequence)	XM_017004806.1
Accession Version	XM_017004806.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGGGGT ACAGGCTCGG GAGAAGCAGC CTGCAGAGCC CCCAGCCCCT 
CTGCGGAGGC GGGCGGCCAG TGATGGACAG TATGAGAACC AGTCTCCAGA AGCCACATCC 
CCTCGTAGCC CTGGGGTTCG CTCCCCTGTC CAGTGTGTCT CCCCGGAGCT GGCTCTTACC 
ATCGCTCTCA ATCCTGGAGG GCGGCCCAAA GAGCCCCATT TGCACAGCTA CAAGGAGGCC 
TTCGAGGAGA TGGAGGGAAC CTCCCCGAGC AGCCCACCAC CCAGTGGGGT GCGGTCCCCC 
CCGGGTCTGG CCAAGACACC CCTGTCTGCT CTGGGCCTGA AACCTCACAA CCCAGCGGAC 
ATCCTGTTGC ACCCCACAGG AGAGGAAGAT GAGGGGAAGG TGGTGGTCAG GCTGTCAGAA 
GAGCCCCGGA GCTATGTGGA GTCTGTGGCA CGGACAGCGG TGGCTGGACC CCGAGCTCAG 
GACTCTGAGC CCAAGAGCTT TAGTGCTCCA GCCACCCAGG CCTATGGCCA TGAGATACCC 
CTGAGGAACG GGACCCTGGG TGGCTCCTTT GTCTCCCCCA GCCCCCTCTC CACCAGCAGC 
CCCATCCTCA GTGCTGACAG CACTTCAGTG GGGAGTTTCC CGTCGGGAGA GAGCAGTGAC 
CAGGGTCCCC GGACGCCCAC CCAGCCTCTG TTGGAGTCTG GCTTCCGCTC AGGCAGCCTG 
GGACAGCCCA GCCCGTCTGC CCAGAGAAAC TACCAGAGCT CTTCTCCTCT CCCGACTGTG 
GGCAGTAGCT ACAGCAGCCC CGACTACTCA CTTCAGCATT TCAGCTCCTC TCCGGAAAGC 
CAGGCTCGAG CTCAGTTCAG TGTGGCTGGC GTCCACACGG TGCCTGGGAG CCCTCAGGCG 
CGCCACAGAA CAGTGGGCAC CAACACTCCC CCTAGTCCTG GCTTCGGCTG GCGGGCCATC 
AATCCCAGCA TGGCTGCCCC CAGCAGTCCC AGTTTGAGCC ATCACCAGAT GATGGGTCCA 
CCAGGCACTG GCTTCCATGG TAGCACTGTC TCCAGCCCCC AGAGCAGTGC AGCGACCACC 
CCGGGGAGCC CCAGCCTGTG TCGGCACCCA GCAGGGGTCT ACCAGGTTTC TGGCCTCCAC 
AACAAAGTGG CCACCACCCC GGGGAGTCCC AGCCTGGGCC GGCACCCTGG GGCTCACCAA 
GGCAACCTGG CCTCCGGTCT TCATAGCAAT GCAATAGCCA GCCCTGGAAG CCCCAGCCTG 
GGCCGTCACC TCGGAGGGTC TGGATCTGTG GTTCCCGGCA GCCCCTGCTT GGACCGGCAT 
GTGGCCTATG GTGGCTATTC TACCCCGGAG GATCGGAGAC CCACACTGTC CCGGCAGAGC 
AGTGCCTCTG GCTACCAGGC TCCTTCCACG CCCTCCTTCC CTGTCTCCCC TGCCTACTAC 
CCTGGCCTGA GCAGCCCTGC CACCTCCCCG TCACCAGACT CCGCAGCCTT CCGGCAAGGG 
AGCCCAACAC CAGCCTTGCC AGAGAAGCGA AGGATGTCAG TGGGAGACCG GGCAGGCAGC 
CTCCCCAACT ATGCCACCAT CAATGGGAAG GTGTCTTCGC CTGTCGCCAG CGGCATGTCC 
AGTCCCAGTG GGGGCAGCAC CGTCTCCTTC TCCCACACTC TGCCCGACTT CTCCAAGTAC 
TCCATGCCAG ACAACAGCCC GGAGACGCGG GCTAAAGTGA AGTTTGTCCA GGACACTTCT 
AAGTATTGGT ACAAGCCTGA GATCTCCAGG GAGCAGGCCA TCGCGCTCCT CAAGGACCAG 
GAGCCGGGGG CCTTCATCAT CCGCGACAGT CACTCCTTCC GAGGCGCGTA CGGGCTGGCC 
ATGAAGGTGT CTTCGCCACC TCCAACCATC ATGCAGCAGA ATAAAAAAGG AGACATGACC 
CATGAGCTGG TCAGGCATTT TCTGATAGAG ACTGGCCCCA GAGGAGTCAA GCTCAAGGGC 
TGCCCCAATG AGCCAAACTT CGGATCGCTG TCTGCCCTGG TCTACCAGCA CTCCATCATC 
CCATTGGCCC TGCCTTGCAA GCTGGTCATT CCAAACCGAG ACCCCACAGA TGAATCGAAA 
GATAGCTCCG GCCCTGCCAA CTCAACTGCA GACCTGCTGA AACAAGGGGC AGCCTGCAAT 
GTGCTCTTCG TCAACTCTGT GGACATGGAG TCACTCACTG GGCCACAGGC CATCTCTAAA 
GCCACATCTG AGACGTTGGC TGCAGACCCC ACGCCAGCTG CCACCATCGT TCACTTCAAA 
GTCTCTGCCC AGGGAATCAC TCTGACTGAC AACCAGAGAA AGCTCTTTTT CAGACGCCAC 
TACCCTCTCA ACACTGTCAC CTTCTGTGAC CTGGATCCAC AGGAAAGAAA GTGGATGAAA 
ACAGAGGGTG GTGCCCCTGC TAAGCTCTTC GGCTTCGTGG CCCGGAAGCA GGGCAGCACC 
ACGGACAACG CCTGCCACCT CTTTGCTGAG CTTGACCCCA ACCAGCCGGC CTCTGCCATC 
GTCAACTTCG TCTCCAAGGT CATGCTGAAT GCCGGCCAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860295.1
CDS	55..5502
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCINGNKQNTNSQSIGSISEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004806.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004806.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGGGGT ACAGGCTCGG GAGAAGCAGC CTGCAGAGCC CCCAGCCCCT 
CTGCGGAGGC GGGCGGCCAG TGATGGACAG TATGAGAACC AGTCTCCAGA AGCCACATCC 
CCTCGTAGCC CTGGGGTTCG CTCCCCTGTC CAGTGTGTCT CCCCGGAGCT GGCTCTTACC 
ATCGCTCTCA ATCCTGGAGG GCGGCCCAAA GAGCCCCATT TGCACAGCTA CAAGGAGGCC 
TTCGAGGAGA TGGAGGGAAC CTCCCCGAGC AGCCCACCAC CCAGTGGGGT GCGGTCCCCC 
CCGGGTCTGG CCAAGACACC CCTGTCTGCT CTGGGCCTGA AACCTCACAA CCCAGCGGAC 
ATCCTGTTGC ACCCCACAGG AGAGGAAGAT GAGGGGAAGG TGGTGGTCAG GCTGTCAGAA 
GAGCCCCGGA GCTATGTGGA GTCTGTGGCA CGGACAGCGG TGGCTGGACC CCGAGCTCAG 
GACTCTGAGC CCAAGAGCTT TAGTGCTCCA GCCACCCAGG CCTATGGCCA TGAGATACCC 
CTGAGGAACG GGACCCTGGG TGGCTCCTTT GTCTCCCCCA GCCCCCTCTC CACCAGCAGC 
CCCATCCTCA GTGCTGACAG CACTTCAGTG GGGAGTTTCC CGTCGGGAGA GAGCAGTGAC 
CAGGGTCCCC GGACGCCCAC CCAGCCTCTG TTGGAGTCTG GCTTCCGCTC AGGCAGCCTG 
GGACAGCCCA GCCCGTCTGC CCAGAGAAAC TACCAGAGCT CTTCTCCTCT CCCGACTGTG 
GGCAGTAGCT ACAGCAGCCC CGACTACTCA CTTCAGCATT TCAGCTCCTC TCCGGAAAGC 
CAGGCTCGAG CTCAGTTCAG TGTGGCTGGC GTCCACACGG TGCCTGGGAG CCCTCAGGCG 
CGCCACAGAA CAGTGGGCAC CAACACTCCC CCTAGTCCTG GCTTCGGCTG GCGGGCCATC 
AATCCCAGCA TGGCTGCCCC CAGCAGTCCC AGTTTGAGCC ATCACCAGAT GATGGGTCCA 
CCAGGCACTG GCTTCCATGG TAGCACTGTC TCCAGCCCCC AGAGCAGTGC AGCGACCACC 
CCGGGGAGCC CCAGCCTGTG TCGGCACCCA GCAGGGGTCT ACCAGGTTTC TGGCCTCCAC 
AACAAAGTGG CCACCACCCC GGGGAGTCCC AGCCTGGGCC GGCACCCTGG GGCTCACCAA 
GGCAACCTGG CCTCCGGTCT TCATAGCAAT GCAATAGCCA GCCCTGGAAG CCCCAGCCTG 
GGCCGTCACC TCGGAGGGTC TGGATCTGTG GTTCCCGGCA GCCCCTGCTT GGACCGGCAT 
GTGGCCTATG GTGGCTATTC TACCCCGGAG GATCGGAGAC CCACACTGTC CCGGCAGAGC 
AGTGCCTCTG GCTACCAGGC TCCTTCCACG CCCTCCTTCC CTGTCTCCCC TGCCTACTAC 
CCTGGCCTGA GCAGCCCTGC CACCTCCCCG TCACCAGACT CCGCAGCCTT CCGGCAAGGG 
AGCCCAACAC CAGCCTTGCC AGAGAAGCGA AGGATGTCAG TGGGAGACCG GGCAGGCAGC 
CTCCCCAACT ATGCCACCAT CAATGGGAAG GTGTCTTCGC CTGTCGCCAG CGGCATGTCC 
AGTCCCAGTG GGGGCAGCAC CGTCTCCTTC TCCCACACTC TGCCCGACTT CTCCAAGTAC 
TCCATGCCAG ACAACAGCCC GGAGACGCGG GCTAAAGTGA AGTTTGTCCA GGACACTTCT 
AAGTATTGGT ACAAGCCTGA GATCTCCAGG GAGCAGGCCA TCGCGCTCCT CAAGGACCAG 
GAGCCGGGGG CCTTCATCAT CCGCGACAGT CACTCCTTCC GAGGCGCGTA CGGGCTGGCC 
ATGAAGGTGT CTTCGCCACC TCCAACCATC ATGCAGCAGA ATAAAAAAGG AGACATGACC 
CATGAGCTGG TCAGGCATTT TCTGATAGAG ACTGGCCCCA GAGGAGTCAA GCTCAAGGGC 
TGCCCCAATG AGCCAAACTT CGGATCGCTG TCTGCCCTGG TCTACCAGCA CTCCATCATC 
CCATTGGCCC TGCCTTGCAA GCTGGTCATT CCAAACCGAG ACCCCACAGA TGAATCGAAA 
GATAGCTCCG GCCCTGCCAA CTCAACTGCA GACCTGCTGA AACAAGGGGC AGCCTGCAAT 
GTGCTCTTCG TCAACTCTGT GGACATGGAG TCACTCACTG GGCCACAGGC CATCTCTAAA 
GCCACATCTG AGACGTTGGC TGCAGACCCC ACGCCAGCTG CCACCATCGT TCACTTCAAA 
GTCTCTGCCC AGGGAATCAC TCTGACTGAC AACCAGAGAA AGCTCTTTTT CAGACGCCAC 
TACCCTCTCA ACACTGTCAC CTTCTGTGAC CTGGATCCAC AGGAAAGAAA GTGGATGAAA 
ACAGAGGGTG GTGCCCCTGC TAAGCTCTTC GGCTTCGTGG CCCGGAAGCA GGGCAGCACC 
ACGGACAACG CCTGCCACCT CTTTGCTGAG CTTGACCCCA ACCAGCCGGC CTCTGCCATC 
GTCAACTTCG TCTCCAAGGT CATGCTGAAT GCCGGCCAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004807.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860296.1 Homo sapiens tensin 1 (TNS1), transcript variant X10, mRNA.

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CloneID	OHu89839
Clone ID Related Accession (Same CDS sequence)	XM_017004807.1
Accession Version	XM_017004807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGGGGT ACAGGCTCGG GAGAAGCAGC CTGCAGAGCC CCCAGCCCCT 
CTGCGGAGGC GGGCGGCCAG TGATGGACAG TATGAGAACC AGTCTCCAGA AGCCACATCC 
CCTCGTAGCC CTGGGGTTCG CTCCCCTGTC CAGTGTGTCT CCCCGGAGCT GGCTCTTACC 
ATCGCTCTCA ATCCTGGAGG GCGGCCCAAA GAGCCCCATT TGCACAGCTA CAAGGAGGCC 
TTCGAGGAGA TGGAGGGAAC CTCCCCGAGC AGCCCACCAC CCAGTGGGGT GCGGTCCCCC 
CCGGGTCTGG CCAAGACACC CCTGTCTGCT CTGGGCCTGA AACCTCACAA CCCAGCGGAC 
ATCCTGTTGC ACCCCACAGG AGAGCCCCGG AGCTATGTGG AGTCTGTGGC ACGGACAGCG 
GTGGCTGGAC CCCGAGCTCA GGACTCTGAG CCCAAGAGCT TTAGTGCTCC AGCCACCCAG 
GCCTATGGCC ATGAGATACC CCTGAGGAAC GGGACCCTGG GTGGCTCCTT TGTCTCCCCC 
AGCCCCCTCT CCACCAGCAG CCCCATCCTC AGTGCTGACA GCACTTCAGT GGGGAGTTTC 
CCGTCGGGAG AGAGCAGTGA CCAGGGTCCC CGGACGCCCA CCCAGCCTCT GTTGGAGTCT 
GGCTTCCGCT CAGGCAGCCT GGGACAGCCC AGCCCGTCTG CCCAGAGAAA CTACCAGAGC 
TCTTCTCCTC TCCCGACTGT GGGCAGTAGC TACAGCAGCC CCGACTACTC ACTTCAGCAT 
TTCAGCTCCT CTCCGGAAAG CCAGGCTCGA GCTCAGTTCA GTGTGGCTGG CGTCCACACG 
GTGCCTGGGA GCCCTCAGGC GCGCCACAGA ACAGTGGGCA CCAACACTCC CCCTAGTCCT 
GGCTTCGGCT GGCGGGCCAT CAATCCCAGC ATGGCTGCCC CCAGCAGTCC CAGTTTGAGC 
CATCACCAGA TGATGGGTCC ACCAGGCACT GGCTTCCATG GTAGCACTGT CTCCAGCCCC 
CAGAGCAGTG CAGCGACCAC CCCGGGGAGC CCCAGCCTGT GTCGGCACCC AGCAGGGGTC 
TACCAGGTTT CTGGCCTCCA CAACAAAGTG GCCACCACCC CGGGGAGTCC CAGCCTGGGC 
CGGCACCCTG GGGCTCACCA AGGCAACCTG GCCTCCGGTC TTCATAGCAA TGCAATAGCC 
AGCCCTGGAA GCCCCAGCCT GGGCCGTCAC CTCGGAGGGT CTGGATCTGT GGTTCCCGGC 
AGCCCCTGCT TGGACCGGCA TGTGGCCTAT GGTGGCTATT CTACCCCGGA GGATCGGAGA 
CCCACACTGT CCCGGCAGAG CAGTGCCTCT GGCTACCAGG CTCCTTCCAC GCCCTCCTTC 
CCTGTCTCCC CTGCCTACTA CCCTGGCCTG AGCAGCCCTG CCACCTCCCC GTCACCAGAC 
TCCGCAGCCT TCCGGCAAGG GAGCCCAACA CCAGCCTTGC CAGAGAAGCG AAGGATGTCA 
GTGGGAGACC GGGCAGGCAG CCTCCCCAAC TATGCCACCA TCAATGGGAA GGTGTCTTCG 
CCTGTCGCCA GCGGCATGTC CAGTCCCAGT GGGGGCAGCA CCGTCTCCTT CTCCCACACT 
CTGCCCGACT TCTCCAAGTA CTCCATGCCA GACAACAGCC CGGAGACGCG GGCTAAAGTG 
AAGTTTGTCC AGGACACTTC TAAGTATTGG TACAAGCCTG AGATCTCCAG GGAGCAGGCC 
ATCGCGCTCC TCAAGGACCA GGAGCCGGGG GCCTTCATCA TCCGCGACAG TCACTCCTTC 
CGAGGCGCGT ACGGGCTGGC CATGAAGGTG TCTTCGCCAC CTCCAACCAT CATGCAGCAG 
AATAAAAAAG GAGACATGAC CCATGAGCTG GTCAGGCATT TTCTGATAGA GACTGGCCCC 
AGAGGAGTCA AGCTCAAGGG CTGCCCCAAT GAGCCAAACT TCGGATCGCT GTCTGCCCTG 
GTCTACCAGC ACTCCATCAT CCCATTGGCC CTGCCTTGCA AGCTGGTCAT TCCAAACCGA 
GACCCCACAG ATGAATCGAA AGATAGCTCC GGCCCTGCCA ACTCAACTGC AGACCTGCTG 
AAACAAGGGG CAGCCTGCAA TGTGCTCTTC GTCAACTCTG TGGACATGGA GTCACTCACT 
GGGCCACAGG CCATCTCTAA AGCCACATCT GAGACGTTGG CTGCAGACCC CACGCCAGCT 
GCCACCATCG TTCACTTCAA AGTCTCTGCC CAGGGAATCA CTCTGACTGA CAACCAGAGA 
AAGCTCTTTT TCAGACGCCA CTACCCTCTC AACACTGTCA CCTTCTGTGA CCTGGATCCA 
CAGGAAAGAA AGTGGATGAA AACAGAGGGT GGTGCCCCTG CTAAGCTCTT CGGCTTCGTG 
GCCCGGAAGC AGGGCAGCAC CACGGACAAC GCCTGCCACC TCTTTGCTGA GCTTGACCCC 
AACCAGCCGG CCTCTGCCAT CGTCAACTTC GTCTCCAAGG TCATGCTGAA TGCCGGCCAA 
AAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860296.1
CDS	55..5463
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCINGNKQNTNSQSIGSISEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004807.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004807.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGGGGT ACAGGCTCGG GAGAAGCAGC CTGCAGAGCC CCCAGCCCCT 
CTGCGGAGGC GGGCGGCCAG TGATGGACAG TATGAGAACC AGTCTCCAGA AGCCACATCC 
CCTCGTAGCC CTGGGGTTCG CTCCCCTGTC CAGTGTGTCT CCCCGGAGCT GGCTCTTACC 
ATCGCTCTCA ATCCTGGAGG GCGGCCCAAA GAGCCCCATT TGCACAGCTA CAAGGAGGCC 
TTCGAGGAGA TGGAGGGAAC CTCCCCGAGC AGCCCACCAC CCAGTGGGGT GCGGTCCCCC 
CCGGGTCTGG CCAAGACACC CCTGTCTGCT CTGGGCCTGA AACCTCACAA CCCAGCGGAC 
ATCCTGTTGC ACCCCACAGG AGAGCCCCGG AGCTATGTGG AGTCTGTGGC ACGGACAGCG 
GTGGCTGGAC CCCGAGCTCA GGACTCTGAG CCCAAGAGCT TTAGTGCTCC AGCCACCCAG 
GCCTATGGCC ATGAGATACC CCTGAGGAAC GGGACCCTGG GTGGCTCCTT TGTCTCCCCC 
AGCCCCCTCT CCACCAGCAG CCCCATCCTC AGTGCTGACA GCACTTCAGT GGGGAGTTTC 
CCGTCGGGAG AGAGCAGTGA CCAGGGTCCC CGGACGCCCA CCCAGCCTCT GTTGGAGTCT 
GGCTTCCGCT CAGGCAGCCT GGGACAGCCC AGCCCGTCTG CCCAGAGAAA CTACCAGAGC 
TCTTCTCCTC TCCCGACTGT GGGCAGTAGC TACAGCAGCC CCGACTACTC ACTTCAGCAT 
TTCAGCTCCT CTCCGGAAAG CCAGGCTCGA GCTCAGTTCA GTGTGGCTGG CGTCCACACG 
GTGCCTGGGA GCCCTCAGGC GCGCCACAGA ACAGTGGGCA CCAACACTCC CCCTAGTCCT 
GGCTTCGGCT GGCGGGCCAT CAATCCCAGC ATGGCTGCCC CCAGCAGTCC CAGTTTGAGC 
CATCACCAGA TGATGGGTCC ACCAGGCACT GGCTTCCATG GTAGCACTGT CTCCAGCCCC 
CAGAGCAGTG CAGCGACCAC CCCGGGGAGC CCCAGCCTGT GTCGGCACCC AGCAGGGGTC 
TACCAGGTTT CTGGCCTCCA CAACAAAGTG GCCACCACCC CGGGGAGTCC CAGCCTGGGC 
CGGCACCCTG GGGCTCACCA AGGCAACCTG GCCTCCGGTC TTCATAGCAA TGCAATAGCC 
AGCCCTGGAA GCCCCAGCCT GGGCCGTCAC CTCGGAGGGT CTGGATCTGT GGTTCCCGGC 
AGCCCCTGCT TGGACCGGCA TGTGGCCTAT GGTGGCTATT CTACCCCGGA GGATCGGAGA 
CCCACACTGT CCCGGCAGAG CAGTGCCTCT GGCTACCAGG CTCCTTCCAC GCCCTCCTTC 
CCTGTCTCCC CTGCCTACTA CCCTGGCCTG AGCAGCCCTG CCACCTCCCC GTCACCAGAC 
TCCGCAGCCT TCCGGCAAGG GAGCCCAACA CCAGCCTTGC CAGAGAAGCG AAGGATGTCA 
GTGGGAGACC GGGCAGGCAG CCTCCCCAAC TATGCCACCA TCAATGGGAA GGTGTCTTCG 
CCTGTCGCCA GCGGCATGTC CAGTCCCAGT GGGGGCAGCA CCGTCTCCTT CTCCCACACT 
CTGCCCGACT TCTCCAAGTA CTCCATGCCA GACAACAGCC CGGAGACGCG GGCTAAAGTG 
AAGTTTGTCC AGGACACTTC TAAGTATTGG TACAAGCCTG AGATCTCCAG GGAGCAGGCC 
ATCGCGCTCC TCAAGGACCA GGAGCCGGGG GCCTTCATCA TCCGCGACAG TCACTCCTTC 
CGAGGCGCGT ACGGGCTGGC CATGAAGGTG TCTTCGCCAC CTCCAACCAT CATGCAGCAG 
AATAAAAAAG GAGACATGAC CCATGAGCTG GTCAGGCATT TTCTGATAGA GACTGGCCCC 
AGAGGAGTCA AGCTCAAGGG CTGCCCCAAT GAGCCAAACT TCGGATCGCT GTCTGCCCTG 
GTCTACCAGC ACTCCATCAT CCCATTGGCC CTGCCTTGCA AGCTGGTCAT TCCAAACCGA 
GACCCCACAG ATGAATCGAA AGATAGCTCC GGCCCTGCCA ACTCAACTGC AGACCTGCTG 
AAACAAGGGG CAGCCTGCAA TGTGCTCTTC GTCAACTCTG TGGACATGGA GTCACTCACT 
GGGCCACAGG CCATCTCTAA AGCCACATCT GAGACGTTGG CTGCAGACCC CACGCCAGCT 
GCCACCATCG TTCACTTCAA AGTCTCTGCC CAGGGAATCA CTCTGACTGA CAACCAGAGA 
AAGCTCTTTT TCAGACGCCA CTACCCTCTC AACACTGTCA CCTTCTGTGA CCTGGATCCA 
CAGGAAAGAA AGTGGATGAA AACAGAGGGT GGTGCCCCTG CTAAGCTCTT CGGCTTCGTG 
GCCCGGAAGC AGGGCAGCAC CACGGACAAC GCCTGCCACC TCTTTGCTGA GCTTGACCCC 
AACCAGCCGG CCTCTGCCAT CGTCAACTTC GTCTCCAAGG TCATGCTGAA TGCCGGCCAA 
AAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004808.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860297.1 Homo sapiens tensin 1 (TNS1), transcript variant X11, mRNA.

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CloneID	OHu89840
Clone ID Related Accession (Same CDS sequence)	XM_017004808.1 , XM_024453077.1
Accession Version	XM_017004808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGGGGTACAG 
GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCCCCTCTGC GGAGGCGGGC GGCCAGTGAT 
GGACAGTATG AGAACCAGTC TCCAGAAGCC ACATCCCCTC GTAGCCCTGG GGTTCGCTCC 
CCTGTCCAGT GTGTCTCCCC GGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGG 
CCCAAAGAGC CCCATTTGCA CAGCTACAAG GAGGCCTTCG AGGAGATGGA GGGAACCTCC 
CCGAGCAGCC CACCACCCAG TGGGGTGCGG TCCCCCCCGG GTCTGGCCAA GACACCCCTG 
TCTGCTCTGG GCCTGAAACC TCACAACCCA GCGGACATCC TGTTGCACCC CACAGGAGAG 
GAAGATGAGG GGAAGGTGGT GGTCAGGCTG TCAGAAGAGC CCCGGAGCTA TGTGGAGTCT 
GTGGCACGGA CAGCGGTGGC TGGACCCCGA GCTCAGGACT CTGAGCCCAA GAGCTTTAGT 
GCTCCAGCCA CCCAGGCCTA TGGCCATGAG ATACCCCTGA GGAACGGGAC CCTGGGTGGC 
TCCTTTGTCT CCCCCAGCCC CCTCTCCACC AGCAGCCCCA TCCTCAGTGC TGACAGCACT 
TCAGTGGGGA GTTTCCCGTC GGGAGAGAGC AGTGACCAGG GTCCCCGGAC GCCCACCCAG 
CCTCTGTTGG AGTCTGGCTT CCGCTCAGGC AGCCTGGGAC AGCCCAGCCC GTCTGCCCAG 
AGAAACTACC AGAGCTCTTC TCCTCTCCCG ACTGTGGGCA GTAGCTACAG CAGCCCCGAC 
TACTCACTTC AGCATTTCAG CTCCTCTCCG GAAAGCCAGG CTCGAGCTCA GTTCAGTGTG 
GCTGGCGTCC ACACGGTGCC TGGGAGCCCT CAGGCGCGCC ACAGAACAGT GGGCACCAAC 
ACTCCCCCTA GTCCTGGCTT CGGCTGGCGG GCCATCAATC CCAGCATGGC TGCCCCCAGC 
AGTCCCAGTT TGAGCCATCA CCAGATGATG GGTCCACCAG GCACTGGCTT CCATGGTAGC 
ACTGTCTCCA GCCCCCAGAG CAGTGCAGCG ACCACCCCGG GGAGCCCCAG CCTGTGTCGG 
CACCCAGCAG GGGTCTACCA GGTTTCTGGC CTCCACAACA AAGTGGCCAC CACCCCGGGG 
AGTCCCAGCC TGGGCCGGCA CCCTGGGGCT CACCAAGGCA ACCTGGCCTC CGGTCTTCAT 
AGCAATGCAA TAGCCAGCCC TGGAAGCCCC AGCCTGGGCC GTCACCTCGG AGGGTCTGGA 
TCTGTGGTTC CCGGCAGCCC CTGCTTGGAC CGGCATGTGG CCTATGGTGG CTATTCTACC 
CCGGAGGATC GGAGACCCAC ACTGTCCCGG CAGAGCAGTG CCTCTGGCTA CCAGGCTCCT 
TCCACGCCCT CCTTCCCTGT CTCCCCTGCC TACTACCCTG GCCTGAGCAG CCCTGCCACC 
TCCCCGTCAC CAGACTCCGC AGCCTTCCGG CAAGGGAGCC CAACACCAGC CTTGCCAGAG 
AAGCGAAGGA TGTCAGTGGG AGACCGGGCA GGCAGCCTCC CCAACTATGC CACCATCAAT 
GGGAAGGTGT CTTCGCCTGT CGCCAGCGGC ATGTCCAGTC CCAGTGGGGG CAGCACCGTC 
TCCTTCTCCC ACACTCTGCC CGACTTCTCC AAGTACTCCA TGCCAGACAA CAGCCCGGAG 
ACGCGGGCTA AAGTGAAGTT TGTCCAGGAC ACTTCTAAGT ATTGGTACAA GCCTGAGATC 
TCCAGGGAGC AGGCCATCGC GCTCCTCAAG GACCAGGAGC CGGGGGCCTT CATCATCCGC 
GACAGTCACT CCTTCCGAGG CGCGTACGGG CTGGCCATGA AGGTGTCTTC GCCACCTCCA 
ACCATCATGC AGCAGAATAA AAAAGGAGAC ATGACCCATG AGCTGGTCAG GCATTTTCTG 
ATAGAGACTG GCCCCAGAGG AGTCAAGCTC AAGGGCTGCC CCAATGAGCC AAACTTCGGA 
TCGCTGTCTG CCCTGGTCTA CCAGCACTCC ATCATCCCAT TGGCCCTGCC TTGCAAGCTG 
GTCATTCCAA ACCGAGACCC CACAGATGAA TCGAAAGATA GCTCCGGCCC TGCCAACTCA 
ACTGCAGACC TGCTGAAACA AGGGGCAGCC TGCAATGTGC TCTTCGTCAA CTCTGTGGAC 
ATGGAGTCAC TCACTGGGCC ACAGGCCATC TCTAAAGCCA CATCTGAGAC GTTGGCTGCA 
GACCCCACGC CAGCTGCCAC CATCGTTCAC TTCAAAGTCT CTGCCCAGGG AATCACTCTG 
ACTGACAACC AGAGAAAGCT CTTTTTCAGA CGCCACTACC CTCTCAACAC TGTCACCTTC 
TGTGACCTGG ATCCACAGGA AAGAAAGTGG ATGAAAACAG AGGGTGGTGC CCCTGCTAAG 
CTCTTCGGCT TCGTGGCCCG GAAGCAGGGC AGCACCACGG ACAACGCCTG CCACCTCTTT 
GCTGAGCTTG ACCCCAACCA GCCGGCCTCT GCCATCGTCA ACTTCGTCTC CAAGGTCATG 
CTGAATGCCG GCCAAAAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860297.1
CDS	55..5238
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004808.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004808.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGGGGTACAG 
GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCCCCTCTGC GGAGGCGGGC GGCCAGTGAT 
GGACAGTATG AGAACCAGTC TCCAGAAGCC ACATCCCCTC GTAGCCCTGG GGTTCGCTCC 
CCTGTCCAGT GTGTCTCCCC GGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGG 
CCCAAAGAGC CCCATTTGCA CAGCTACAAG GAGGCCTTCG AGGAGATGGA GGGAACCTCC 
CCGAGCAGCC CACCACCCAG TGGGGTGCGG TCCCCCCCGG GTCTGGCCAA GACACCCCTG 
TCTGCTCTGG GCCTGAAACC TCACAACCCA GCGGACATCC TGTTGCACCC CACAGGAGAG 
GAAGATGAGG GGAAGGTGGT GGTCAGGCTG TCAGAAGAGC CCCGGAGCTA TGTGGAGTCT 
GTGGCACGGA CAGCGGTGGC TGGACCCCGA GCTCAGGACT CTGAGCCCAA GAGCTTTAGT 
GCTCCAGCCA CCCAGGCCTA TGGCCATGAG ATACCCCTGA GGAACGGGAC CCTGGGTGGC 
TCCTTTGTCT CCCCCAGCCC CCTCTCCACC AGCAGCCCCA TCCTCAGTGC TGACAGCACT 
TCAGTGGGGA GTTTCCCGTC GGGAGAGAGC AGTGACCAGG GTCCCCGGAC GCCCACCCAG 
CCTCTGTTGG AGTCTGGCTT CCGCTCAGGC AGCCTGGGAC AGCCCAGCCC GTCTGCCCAG 
AGAAACTACC AGAGCTCTTC TCCTCTCCCG ACTGTGGGCA GTAGCTACAG CAGCCCCGAC 
TACTCACTTC AGCATTTCAG CTCCTCTCCG GAAAGCCAGG CTCGAGCTCA GTTCAGTGTG 
GCTGGCGTCC ACACGGTGCC TGGGAGCCCT CAGGCGCGCC ACAGAACAGT GGGCACCAAC 
ACTCCCCCTA GTCCTGGCTT CGGCTGGCGG GCCATCAATC CCAGCATGGC TGCCCCCAGC 
AGTCCCAGTT TGAGCCATCA CCAGATGATG GGTCCACCAG GCACTGGCTT CCATGGTAGC 
ACTGTCTCCA GCCCCCAGAG CAGTGCAGCG ACCACCCCGG GGAGCCCCAG CCTGTGTCGG 
CACCCAGCAG GGGTCTACCA GGTTTCTGGC CTCCACAACA AAGTGGCCAC CACCCCGGGG 
AGTCCCAGCC TGGGCCGGCA CCCTGGGGCT CACCAAGGCA ACCTGGCCTC CGGTCTTCAT 
AGCAATGCAA TAGCCAGCCC TGGAAGCCCC AGCCTGGGCC GTCACCTCGG AGGGTCTGGA 
TCTGTGGTTC CCGGCAGCCC CTGCTTGGAC CGGCATGTGG CCTATGGTGG CTATTCTACC 
CCGGAGGATC GGAGACCCAC ACTGTCCCGG CAGAGCAGTG CCTCTGGCTA CCAGGCTCCT 
TCCACGCCCT CCTTCCCTGT CTCCCCTGCC TACTACCCTG GCCTGAGCAG CCCTGCCACC 
TCCCCGTCAC CAGACTCCGC AGCCTTCCGG CAAGGGAGCC CAACACCAGC CTTGCCAGAG 
AAGCGAAGGA TGTCAGTGGG AGACCGGGCA GGCAGCCTCC CCAACTATGC CACCATCAAT 
GGGAAGGTGT CTTCGCCTGT CGCCAGCGGC ATGTCCAGTC CCAGTGGGGG CAGCACCGTC 
TCCTTCTCCC ACACTCTGCC CGACTTCTCC AAGTACTCCA TGCCAGACAA CAGCCCGGAG 
ACGCGGGCTA AAGTGAAGTT TGTCCAGGAC ACTTCTAAGT ATTGGTACAA GCCTGAGATC 
TCCAGGGAGC AGGCCATCGC GCTCCTCAAG GACCAGGAGC CGGGGGCCTT CATCATCCGC 
GACAGTCACT CCTTCCGAGG CGCGTACGGG CTGGCCATGA AGGTGTCTTC GCCACCTCCA 
ACCATCATGC AGCAGAATAA AAAAGGAGAC ATGACCCATG AGCTGGTCAG GCATTTTCTG 
ATAGAGACTG GCCCCAGAGG AGTCAAGCTC AAGGGCTGCC CCAATGAGCC AAACTTCGGA 
TCGCTGTCTG CCCTGGTCTA CCAGCACTCC ATCATCCCAT TGGCCCTGCC TTGCAAGCTG 
GTCATTCCAA ACCGAGACCC CACAGATGAA TCGAAAGATA GCTCCGGCCC TGCCAACTCA 
ACTGCAGACC TGCTGAAACA AGGGGCAGCC TGCAATGTGC TCTTCGTCAA CTCTGTGGAC 
ATGGAGTCAC TCACTGGGCC ACAGGCCATC TCTAAAGCCA CATCTGAGAC GTTGGCTGCA 
GACCCCACGC CAGCTGCCAC CATCGTTCAC TTCAAAGTCT CTGCCCAGGG AATCACTCTG 
ACTGACAACC AGAGAAAGCT CTTTTTCAGA CGCCACTACC CTCTCAACAC TGTCACCTTC 
TGTGACCTGG ATCCACAGGA AAGAAAGTGG ATGAAAACAG AGGGTGGTGC CCCTGCTAAG 
CTCTTCGGCT TCGTGGCCCG GAAGCAGGGC AGCACCACGG ACAACGCCTG CCACCTCTTT 
GCTGAGCTTG ACCCCAACCA GCCGGCCTCT GCCATCGTCA ACTTCGTCTC CAAGGTCATG 
CTGAATGCCG GCCAAAAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453077.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308845.1 Homo sapiens tensin 1 (TNS1), transcript variant X10, mRNA.

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CloneID	OHu89840
Clone ID Related Accession (Same CDS sequence)	XM_017004808.1 , XM_024453077.1
Accession Version	XM_024453077.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGGGGTACAG 
GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCCCCTCTGC GGAGGCGGGC GGCCAGTGAT 
GGACAGTATG AGAACCAGTC TCCAGAAGCC ACATCCCCTC GTAGCCCTGG GGTTCGCTCC 
CCTGTCCAGT GTGTCTCCCC GGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGG 
CCCAAAGAGC CCCATTTGCA CAGCTACAAG GAGGCCTTCG AGGAGATGGA GGGAACCTCC 
CCGAGCAGCC CACCACCCAG TGGGGTGCGG TCCCCCCCGG GTCTGGCCAA GACACCCCTG 
TCTGCTCTGG GCCTGAAACC TCACAACCCA GCGGACATCC TGTTGCACCC CACAGGAGAG 
GAAGATGAGG GGAAGGTGGT GGTCAGGCTG TCAGAAGAGC CCCGGAGCTA TGTGGAGTCT 
GTGGCACGGA CAGCGGTGGC TGGACCCCGA GCTCAGGACT CTGAGCCCAA GAGCTTTAGT 
GCTCCAGCCA CCCAGGCCTA TGGCCATGAG ATACCCCTGA GGAACGGGAC CCTGGGTGGC 
TCCTTTGTCT CCCCCAGCCC CCTCTCCACC AGCAGCCCCA TCCTCAGTGC TGACAGCACT 
TCAGTGGGGA GTTTCCCGTC GGGAGAGAGC AGTGACCAGG GTCCCCGGAC GCCCACCCAG 
CCTCTGTTGG AGTCTGGCTT CCGCTCAGGC AGCCTGGGAC AGCCCAGCCC GTCTGCCCAG 
AGAAACTACC AGAGCTCTTC TCCTCTCCCG ACTGTGGGCA GTAGCTACAG CAGCCCCGAC 
TACTCACTTC AGCATTTCAG CTCCTCTCCG GAAAGCCAGG CTCGAGCTCA GTTCAGTGTG 
GCTGGCGTCC ACACGGTGCC TGGGAGCCCT CAGGCGCGCC ACAGAACAGT GGGCACCAAC 
ACTCCCCCTA GTCCTGGCTT CGGCTGGCGG GCCATCAATC CCAGCATGGC TGCCCCCAGC 
AGTCCCAGTT TGAGCCATCA CCAGATGATG GGTCCACCAG GCACTGGCTT CCATGGTAGC 
ACTGTCTCCA GCCCCCAGAG CAGTGCAGCG ACCACCCCGG GGAGCCCCAG CCTGTGTCGG 
CACCCAGCAG GGGTCTACCA GGTTTCTGGC CTCCACAACA AAGTGGCCAC CACCCCGGGG 
AGTCCCAGCC TGGGCCGGCA CCCTGGGGCT CACCAAGGCA ACCTGGCCTC CGGTCTTCAT 
AGCAATGCAA TAGCCAGCCC TGGAAGCCCC AGCCTGGGCC GTCACCTCGG AGGGTCTGGA 
TCTGTGGTTC CCGGCAGCCC CTGCTTGGAC CGGCATGTGG CCTATGGTGG CTATTCTACC 
CCGGAGGATC GGAGACCCAC ACTGTCCCGG CAGAGCAGTG CCTCTGGCTA CCAGGCTCCT 
TCCACGCCCT CCTTCCCTGT CTCCCCTGCC TACTACCCTG GCCTGAGCAG CCCTGCCACC 
TCCCCGTCAC CAGACTCCGC AGCCTTCCGG CAAGGGAGCC CAACACCAGC CTTGCCAGAG 
AAGCGAAGGA TGTCAGTGGG AGACCGGGCA GGCAGCCTCC CCAACTATGC CACCATCAAT 
GGGAAGGTGT CTTCGCCTGT CGCCAGCGGC ATGTCCAGTC CCAGTGGGGG CAGCACCGTC 
TCCTTCTCCC ACACTCTGCC CGACTTCTCC AAGTACTCCA TGCCAGACAA CAGCCCGGAG 
ACGCGGGCTA AAGTGAAGTT TGTCCAGGAC ACTTCTAAGT ATTGGTACAA GCCTGAGATC 
TCCAGGGAGC AGGCCATCGC GCTCCTCAAG GACCAGGAGC CGGGGGCCTT CATCATCCGC 
GACAGTCACT CCTTCCGAGG CGCGTACGGG CTGGCCATGA AGGTGTCTTC GCCACCTCCA 
ACCATCATGC AGCAGAATAA AAAAGGAGAC ATGACCCATG AGCTGGTCAG GCATTTTCTG 
ATAGAGACTG GCCCCAGAGG AGTCAAGCTC AAGGGCTGCC CCAATGAGCC AAACTTCGGA 
TCGCTGTCTG CCCTGGTCTA CCAGCACTCC ATCATCCCAT TGGCCCTGCC TTGCAAGCTG 
GTCATTCCAA ACCGAGACCC CACAGATGAA TCGAAAGATA GCTCCGGCCC TGCCAACTCA 
ACTGCAGACC TGCTGAAACA AGGGGCAGCC TGCAATGTGC TCTTCGTCAA CTCTGTGGAC 
ATGGAGTCAC TCACTGGGCC ACAGGCCATC TCTAAAGCCA CATCTGAGAC GTTGGCTGCA 
GACCCCACGC CAGCTGCCAC CATCGTTCAC TTCAAAGTCT CTGCCCAGGG AATCACTCTG 
ACTGACAACC AGAGAAAGCT CTTTTTCAGA CGCCACTACC CTCTCAACAC TGTCACCTTC 
TGTGACCTGG ATCCACAGGA AAGAAAGTGG ATGAAAACAG AGGGTGGTGC CCCTGCTAAG 
CTCTTCGGCT TCGTGGCCCG GAAGCAGGGC AGCACCACGG ACAACGCCTG CCACCTCTTT 
GCTGAGCTTG ACCCCAACCA GCCGGCCTCT GCCATCGTCA ACTTCGTCTC CAAGGTCATG 
CTGAATGCCG GCCAAAAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308845.1
CDS	190..5373
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453077.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453077.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGGGGTACAG 
GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCCCCTCTGC GGAGGCGGGC GGCCAGTGAT 
GGACAGTATG AGAACCAGTC TCCAGAAGCC ACATCCCCTC GTAGCCCTGG GGTTCGCTCC 
CCTGTCCAGT GTGTCTCCCC GGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGG 
CCCAAAGAGC CCCATTTGCA CAGCTACAAG GAGGCCTTCG AGGAGATGGA GGGAACCTCC 
CCGAGCAGCC CACCACCCAG TGGGGTGCGG TCCCCCCCGG GTCTGGCCAA GACACCCCTG 
TCTGCTCTGG GCCTGAAACC TCACAACCCA GCGGACATCC TGTTGCACCC CACAGGAGAG 
GAAGATGAGG GGAAGGTGGT GGTCAGGCTG TCAGAAGAGC CCCGGAGCTA TGTGGAGTCT 
GTGGCACGGA CAGCGGTGGC TGGACCCCGA GCTCAGGACT CTGAGCCCAA GAGCTTTAGT 
GCTCCAGCCA CCCAGGCCTA TGGCCATGAG ATACCCCTGA GGAACGGGAC CCTGGGTGGC 
TCCTTTGTCT CCCCCAGCCC CCTCTCCACC AGCAGCCCCA TCCTCAGTGC TGACAGCACT 
TCAGTGGGGA GTTTCCCGTC GGGAGAGAGC AGTGACCAGG GTCCCCGGAC GCCCACCCAG 
CCTCTGTTGG AGTCTGGCTT CCGCTCAGGC AGCCTGGGAC AGCCCAGCCC GTCTGCCCAG 
AGAAACTACC AGAGCTCTTC TCCTCTCCCG ACTGTGGGCA GTAGCTACAG CAGCCCCGAC 
TACTCACTTC AGCATTTCAG CTCCTCTCCG GAAAGCCAGG CTCGAGCTCA GTTCAGTGTG 
GCTGGCGTCC ACACGGTGCC TGGGAGCCCT CAGGCGCGCC ACAGAACAGT GGGCACCAAC 
ACTCCCCCTA GTCCTGGCTT CGGCTGGCGG GCCATCAATC CCAGCATGGC TGCCCCCAGC 
AGTCCCAGTT TGAGCCATCA CCAGATGATG GGTCCACCAG GCACTGGCTT CCATGGTAGC 
ACTGTCTCCA GCCCCCAGAG CAGTGCAGCG ACCACCCCGG GGAGCCCCAG CCTGTGTCGG 
CACCCAGCAG GGGTCTACCA GGTTTCTGGC CTCCACAACA AAGTGGCCAC CACCCCGGGG 
AGTCCCAGCC TGGGCCGGCA CCCTGGGGCT CACCAAGGCA ACCTGGCCTC CGGTCTTCAT 
AGCAATGCAA TAGCCAGCCC TGGAAGCCCC AGCCTGGGCC GTCACCTCGG AGGGTCTGGA 
TCTGTGGTTC CCGGCAGCCC CTGCTTGGAC CGGCATGTGG CCTATGGTGG CTATTCTACC 
CCGGAGGATC GGAGACCCAC ACTGTCCCGG CAGAGCAGTG CCTCTGGCTA CCAGGCTCCT 
TCCACGCCCT CCTTCCCTGT CTCCCCTGCC TACTACCCTG GCCTGAGCAG CCCTGCCACC 
TCCCCGTCAC CAGACTCCGC AGCCTTCCGG CAAGGGAGCC CAACACCAGC CTTGCCAGAG 
AAGCGAAGGA TGTCAGTGGG AGACCGGGCA GGCAGCCTCC CCAACTATGC CACCATCAAT 
GGGAAGGTGT CTTCGCCTGT CGCCAGCGGC ATGTCCAGTC CCAGTGGGGG CAGCACCGTC 
TCCTTCTCCC ACACTCTGCC CGACTTCTCC AAGTACTCCA TGCCAGACAA CAGCCCGGAG 
ACGCGGGCTA AAGTGAAGTT TGTCCAGGAC ACTTCTAAGT ATTGGTACAA GCCTGAGATC 
TCCAGGGAGC AGGCCATCGC GCTCCTCAAG GACCAGGAGC CGGGGGCCTT CATCATCCGC 
GACAGTCACT CCTTCCGAGG CGCGTACGGG CTGGCCATGA AGGTGTCTTC GCCACCTCCA 
ACCATCATGC AGCAGAATAA AAAAGGAGAC ATGACCCATG AGCTGGTCAG GCATTTTCTG 
ATAGAGACTG GCCCCAGAGG AGTCAAGCTC AAGGGCTGCC CCAATGAGCC AAACTTCGGA 
TCGCTGTCTG CCCTGGTCTA CCAGCACTCC ATCATCCCAT TGGCCCTGCC TTGCAAGCTG 
GTCATTCCAA ACCGAGACCC CACAGATGAA TCGAAAGATA GCTCCGGCCC TGCCAACTCA 
ACTGCAGACC TGCTGAAACA AGGGGCAGCC TGCAATGTGC TCTTCGTCAA CTCTGTGGAC 
ATGGAGTCAC TCACTGGGCC ACAGGCCATC TCTAAAGCCA CATCTGAGAC GTTGGCTGCA 
GACCCCACGC CAGCTGCCAC CATCGTTCAC TTCAAAGTCT CTGCCCAGGG AATCACTCTG 
ACTGACAACC AGAGAAAGCT CTTTTTCAGA CGCCACTACC CTCTCAACAC TGTCACCTTC 
TGTGACCTGG ATCCACAGGA AAGAAAGTGG ATGAAAACAG AGGGTGGTGC CCCTGCTAAG 
CTCTTCGGCT TCGTGGCCCG GAAGCAGGGC AGCACCACGG ACAACGCCTG CCACCTCTTT 
GCTGAGCTTG ACCCCAACCA GCCGGCCTCT GCCATCGTCA ACTTCGTCTC CAAGGTCATG 
CTGAATGCCG GCCAAAAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004810.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860299.1 Homo sapiens tensin 1 (TNS1), transcript variant X13, mRNA.

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CloneID	OHu89841
Clone ID Related Accession (Same CDS sequence)	XM_017004810.1 , XM_017004809.1
Accession Version	XM_017004810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGCGTA CAACGCCAGG CTGCAGGGCA TCGGACACAG TGGCAGCTTC 
CCGCCCCCTC CTCTCCACCG GCTCCAATCT TCCTCCCTCT CTGGGGTACA GGCTCGGGAG 
AAGCAGCCTG CAGAGCCCCC AGCCCCTCTG CGGAGGCGGG CGGCCAGTGA TGGACAGTAT 
GAGAACCAGT CTCCAGAAGC CACATCCCCT CGTAGCCCTG GGGTTCGCTC CCCTGTCCAG 
TGTGTCTCCC CGGAGCTGGC TCTTACCATC GCTCTCAATC CTGGAGGGCG GCCCAAAGAG 
CCCCATTTGC ACAGCTACAA GGAGGCCTTC GAGGAGATGG AGGGAACCTC CCCGAGCAGC 
CCACCACCCA GTGGGGTGCG GTCCCCCCCG GGTCTGGCCA AGACACCCCT GTCTGCTCTG 
GGCCTGAAAC CTCACAACCC AGCGGACATC CTGTTGCACC CCACAGGAGT TACCAGAAGA 
CGGATCCAGC CAGATTCTCT TTCTCAAGAA ATAATCCCTG TTAATCTCTT TCTCTCTTTT 
TCCTTTTTTG CTCTTCCTCA TCTTCACCAC CTGCCCTTCT TTCCTGCCTC TCCTTCCTCC 
CACCTCTCTT CTCGTCTTCC CTCAGAGGAA GATGAGGGGA AGGTGGTGGT CAGGCTGTCA 
GAAGAGCCCC GGAGCTATGT GGAGTCTGTG GCACGGACAG CGGTGGCTGG ACCCCGAGCT 
CAGGACTCTG AGCCCAAGAG CTTTAGTGCT CCAGCCACCC AGGCCTATGG CCATGAGATA 
CCCCTGAGGA ACGGGACCCT GGGTGGCTCC TTTGTCTCCC CCAGCCCCCT CTCCACCAGC 
AGCCCCATCC TCAGTGCTGA CAGCACTTCA GTGGGGAGTT TCCCGTCGGG AGAGAGCAGT 
GACCAGGGTC CCCGGACGCC CACCCAGCCT CTGTTGGAGT CTGGCTTCCG CTCAGGCAGC 
CTGGGACAGC CCAGCCCGTC TGCCCAGAGA AACTACCAGA GCTCTTCTCC TCTCCCGACT 
GTGGGCAGTA GCTACAGCAG CCCCGACTAC TCACTTCAGC ATTTCAGCTC CTCTCCGGAA 
AGCCAGGCTC GAGCTCAGTT CAGTGTGGCT GGCGTCCACA CGGTGCCTGG GAGCCCTCAG 
GCGCGCCACA GAACAGTGGG CACCAACACT CCCCCTAGTC CTGGCTTCGG CTGGCGGGCC 
ATCAATCCCA GCATGGCTGC CCCCAGCAGT CCCAGTTTGA GCCATCACCA GATGATGGGT 
CCACCAGGCA CTGGCTTCCA TGGTAGCACT GTCTCCAGCC CCCAGAGCAG TGCAGCGACC 
ACCCCGGGGA GCCCCAGCCT GTGTCGGCAC CCAGCAGGGG TCTACCAGGT TTCTGGCCTC 
CACAACAAAG TGGCCACCAC CCCGGGGAGT CCCAGCCTGG GCCGGCACCC TGGGGCTCAC 
CAAGGCAACC TGGCCTCCGG TCTTCATAGC AATGCAATAG CCAGCCCTGG AAGCCCCAGC 
CTGGGCCGTC ACCTCGGAGG GTCTGGATCT GTGGTTCCCG GCAGCCCCTG CTTGGACCGG 
CATGTGGCCT ATGGTGGCTA TTCTACCCCG GAGGATCGGA GACCCACACT GTCCCGGCAG 
AGCAGTGCCT CTGGCTACCA GGCTCCTTCC ACGCCCTCCT TCCCTGTCTC CCCTGCCTAC 
TACCCTGGCC TGAGCAGCCC TGCCACCTCC CCGTCACCAG ACTCCGCAGC CTTCCGGCAA 
GGGAGCCCAA CACCAGCCTT GCCAGAGAAG CGAAGGATGT CAGTGGGAGA CCGGGCAGGC 
AGCCTCCCCA ACTATGCCAC CATCAATGGG AAGGTGTCTT CGCCTGTCGC CAGCGGCATG 
TCCAGTCCCA GTGGGGGCAG CACCGTCTCC TTCTCCCACA CTCTGCCCGA CTTCTCCAAG 
TACTCCATGC CAGACAACAG CCCGGAGACG CGGGCTAAAG TGAAGTTTGT CCAGGACACT 
TCTAAGTATT GGTACAAGCC TGAGATCTCC AGGGAGCAGG CCATCGCGCT CCTCAAGGAC 
CAGGAGCCGG GGGCCTTCAT CATCCGCGAC AGTCACTCCT TCCGAGGCGC GTACGGGCTG 
GCCATGAAGG TGTCTTCGCC ACCTCCAACC ATCATGCAGC AGAATAAAAA AGGAGACATG 
ACCCATGAGC TGGTCAGGCA TTTTCTGATA GAGACTGGCC CCAGAGGAGT CAAGCTCAAG 
GGCTGCCCCA ATGAGCCAAA CTTCGGATCG CTGTCTGCCC TGGTCTACCA GCACTCCATC 
ATCCCATTGG CCCTGCCTTG CAAGCTGGTC ATTCCAAACC GAGACCCCAC AGATGAATCG 
AAAGATAGCT CCGGCCCTGC CAACTCAACT GCAGACCTGC TGAAACAAGG GGCAGCCTGC 
AATGTGCTCT TCGTCAACTC TGTGGACATG GAGTCACTCA CTGGGCCACA GGCCATCTCT 
AAAGCCACAT CTGAGACGTT GGCTGCAGAC CCCACGCCAG CTGCCACCAT CGTTCACTTC 
AAAGTCTCTG CCCAGGGAAT CACTCTGACT GACAACCAGA GAAAGCTCTT TTTCAGACGC 
CACTACCCTC TCAACACTGT CACCTTCTGT GACCTGGATC CACAGGAAAG AAAGTGGATG 
AAAACAGAGG GTGGTGCCCC TGCTAAGCTC TTCGGCTTCG TGGCCCGGAA GCAGGGCAGC 
ACCACGGACA ACGCCTGCCA CCTCTTTGCT GAGCTTGACC CCAACCAGCC GGCCTCTGCC 
ATCGTCAACT TCGTCTCCAA GGTCATGCTG AATGCCGGCC AAAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860299.1
CDS	279..5969
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCINGNKQNTNSQSIGSISEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004810.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004810.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGCGTA CAACGCCAGG CTGCAGGGCA TCGGACACAG TGGCAGCTTC 
CCGCCCCCTC CTCTCCACCG GCTCCAATCT TCCTCCCTCT CTGGGGTACA GGCTCGGGAG 
AAGCAGCCTG CAGAGCCCCC AGCCCCTCTG CGGAGGCGGG CGGCCAGTGA TGGACAGTAT 
GAGAACCAGT CTCCAGAAGC CACATCCCCT CGTAGCCCTG GGGTTCGCTC CCCTGTCCAG 
TGTGTCTCCC CGGAGCTGGC TCTTACCATC GCTCTCAATC CTGGAGGGCG GCCCAAAGAG 
CCCCATTTGC ACAGCTACAA GGAGGCCTTC GAGGAGATGG AGGGAACCTC CCCGAGCAGC 
CCACCACCCA GTGGGGTGCG GTCCCCCCCG GGTCTGGCCA AGACACCCCT GTCTGCTCTG 
GGCCTGAAAC CTCACAACCC AGCGGACATC CTGTTGCACC CCACAGGAGT TACCAGAAGA 
CGGATCCAGC CAGATTCTCT TTCTCAAGAA ATAATCCCTG TTAATCTCTT TCTCTCTTTT 
TCCTTTTTTG CTCTTCCTCA TCTTCACCAC CTGCCCTTCT TTCCTGCCTC TCCTTCCTCC 
CACCTCTCTT CTCGTCTTCC CTCAGAGGAA GATGAGGGGA AGGTGGTGGT CAGGCTGTCA 
GAAGAGCCCC GGAGCTATGT GGAGTCTGTG GCACGGACAG CGGTGGCTGG ACCCCGAGCT 
CAGGACTCTG AGCCCAAGAG CTTTAGTGCT CCAGCCACCC AGGCCTATGG CCATGAGATA 
CCCCTGAGGA ACGGGACCCT GGGTGGCTCC TTTGTCTCCC CCAGCCCCCT CTCCACCAGC 
AGCCCCATCC TCAGTGCTGA CAGCACTTCA GTGGGGAGTT TCCCGTCGGG AGAGAGCAGT 
GACCAGGGTC CCCGGACGCC CACCCAGCCT CTGTTGGAGT CTGGCTTCCG CTCAGGCAGC 
CTGGGACAGC CCAGCCCGTC TGCCCAGAGA AACTACCAGA GCTCTTCTCC TCTCCCGACT 
GTGGGCAGTA GCTACAGCAG CCCCGACTAC TCACTTCAGC ATTTCAGCTC CTCTCCGGAA 
AGCCAGGCTC GAGCTCAGTT CAGTGTGGCT GGCGTCCACA CGGTGCCTGG GAGCCCTCAG 
GCGCGCCACA GAACAGTGGG CACCAACACT CCCCCTAGTC CTGGCTTCGG CTGGCGGGCC 
ATCAATCCCA GCATGGCTGC CCCCAGCAGT CCCAGTTTGA GCCATCACCA GATGATGGGT 
CCACCAGGCA CTGGCTTCCA TGGTAGCACT GTCTCCAGCC CCCAGAGCAG TGCAGCGACC 
ACCCCGGGGA GCCCCAGCCT GTGTCGGCAC CCAGCAGGGG TCTACCAGGT TTCTGGCCTC 
CACAACAAAG TGGCCACCAC CCCGGGGAGT CCCAGCCTGG GCCGGCACCC TGGGGCTCAC 
CAAGGCAACC TGGCCTCCGG TCTTCATAGC AATGCAATAG CCAGCCCTGG AAGCCCCAGC 
CTGGGCCGTC ACCTCGGAGG GTCTGGATCT GTGGTTCCCG GCAGCCCCTG CTTGGACCGG 
CATGTGGCCT ATGGTGGCTA TTCTACCCCG GAGGATCGGA GACCCACACT GTCCCGGCAG 
AGCAGTGCCT CTGGCTACCA GGCTCCTTCC ACGCCCTCCT TCCCTGTCTC CCCTGCCTAC 
TACCCTGGCC TGAGCAGCCC TGCCACCTCC CCGTCACCAG ACTCCGCAGC CTTCCGGCAA 
GGGAGCCCAA CACCAGCCTT GCCAGAGAAG CGAAGGATGT CAGTGGGAGA CCGGGCAGGC 
AGCCTCCCCA ACTATGCCAC CATCAATGGG AAGGTGTCTT CGCCTGTCGC CAGCGGCATG 
TCCAGTCCCA GTGGGGGCAG CACCGTCTCC TTCTCCCACA CTCTGCCCGA CTTCTCCAAG 
TACTCCATGC CAGACAACAG CCCGGAGACG CGGGCTAAAG TGAAGTTTGT CCAGGACACT 
TCTAAGTATT GGTACAAGCC TGAGATCTCC AGGGAGCAGG CCATCGCGCT CCTCAAGGAC 
CAGGAGCCGG GGGCCTTCAT CATCCGCGAC AGTCACTCCT TCCGAGGCGC GTACGGGCTG 
GCCATGAAGG TGTCTTCGCC ACCTCCAACC ATCATGCAGC AGAATAAAAA AGGAGACATG 
ACCCATGAGC TGGTCAGGCA TTTTCTGATA GAGACTGGCC CCAGAGGAGT CAAGCTCAAG 
GGCTGCCCCA ATGAGCCAAA CTTCGGATCG CTGTCTGCCC TGGTCTACCA GCACTCCATC 
ATCCCATTGG CCCTGCCTTG CAAGCTGGTC ATTCCAAACC GAGACCCCAC AGATGAATCG 
AAAGATAGCT CCGGCCCTGC CAACTCAACT GCAGACCTGC TGAAACAAGG GGCAGCCTGC 
AATGTGCTCT TCGTCAACTC TGTGGACATG GAGTCACTCA CTGGGCCACA GGCCATCTCT 
AAAGCCACAT CTGAGACGTT GGCTGCAGAC CCCACGCCAG CTGCCACCAT CGTTCACTTC 
AAAGTCTCTG CCCAGGGAAT CACTCTGACT GACAACCAGA GAAAGCTCTT TTTCAGACGC 
CACTACCCTC TCAACACTGT CACCTTCTGT GACCTGGATC CACAGGAAAG AAAGTGGATG 
AAAACAGAGG GTGGTGCCCC TGCTAAGCTC TTCGGCTTCG TGGCCCGGAA GCAGGGCAGC 
ACCACGGACA ACGCCTGCCA CCTCTTTGCT GAGCTTGACC CCAACCAGCC GGCCTCTGCC 
ATCGTCAACT TCGTCTCCAA GGTCATGCTG AATGCCGGCC AAAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004809.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860298.1 Homo sapiens tensin 1 (TNS1), transcript variant X12, mRNA.

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CloneID	OHu89841
Clone ID Related Accession (Same CDS sequence)	XM_017004810.1 , XM_017004809.1
Accession Version	XM_017004809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGCGTA CAACGCCAGG CTGCAGGGCA TCGGACACAG TGGCAGCTTC 
CCGCCCCCTC CTCTCCACCG GCTCCAATCT TCCTCCCTCT CTGGGGTACA GGCTCGGGAG 
AAGCAGCCTG CAGAGCCCCC AGCCCCTCTG CGGAGGCGGG CGGCCAGTGA TGGACAGTAT 
GAGAACCAGT CTCCAGAAGC CACATCCCCT CGTAGCCCTG GGGTTCGCTC CCCTGTCCAG 
TGTGTCTCCC CGGAGCTGGC TCTTACCATC GCTCTCAATC CTGGAGGGCG GCCCAAAGAG 
CCCCATTTGC ACAGCTACAA GGAGGCCTTC GAGGAGATGG AGGGAACCTC CCCGAGCAGC 
CCACCACCCA GTGGGGTGCG GTCCCCCCCG GGTCTGGCCA AGACACCCCT GTCTGCTCTG 
GGCCTGAAAC CTCACAACCC AGCGGACATC CTGTTGCACC CCACAGGAGT TACCAGAAGA 
CGGATCCAGC CAGATTCTCT TTCTCAAGAA ATAATCCCTG TTAATCTCTT TCTCTCTTTT 
TCCTTTTTTG CTCTTCCTCA TCTTCACCAC CTGCCCTTCT TTCCTGCCTC TCCTTCCTCC 
CACCTCTCTT CTCGTCTTCC CTCAGAGGAA GATGAGGGGA AGGTGGTGGT CAGGCTGTCA 
GAAGAGCCCC GGAGCTATGT GGAGTCTGTG GCACGGACAG CGGTGGCTGG ACCCCGAGCT 
CAGGACTCTG AGCCCAAGAG CTTTAGTGCT CCAGCCACCC AGGCCTATGG CCATGAGATA 
CCCCTGAGGA ACGGGACCCT GGGTGGCTCC TTTGTCTCCC CCAGCCCCCT CTCCACCAGC 
AGCCCCATCC TCAGTGCTGA CAGCACTTCA GTGGGGAGTT TCCCGTCGGG AGAGAGCAGT 
GACCAGGGTC CCCGGACGCC CACCCAGCCT CTGTTGGAGT CTGGCTTCCG CTCAGGCAGC 
CTGGGACAGC CCAGCCCGTC TGCCCAGAGA AACTACCAGA GCTCTTCTCC TCTCCCGACT 
GTGGGCAGTA GCTACAGCAG CCCCGACTAC TCACTTCAGC ATTTCAGCTC CTCTCCGGAA 
AGCCAGGCTC GAGCTCAGTT CAGTGTGGCT GGCGTCCACA CGGTGCCTGG GAGCCCTCAG 
GCGCGCCACA GAACAGTGGG CACCAACACT CCCCCTAGTC CTGGCTTCGG CTGGCGGGCC 
ATCAATCCCA GCATGGCTGC CCCCAGCAGT CCCAGTTTGA GCCATCACCA GATGATGGGT 
CCACCAGGCA CTGGCTTCCA TGGTAGCACT GTCTCCAGCC CCCAGAGCAG TGCAGCGACC 
ACCCCGGGGA GCCCCAGCCT GTGTCGGCAC CCAGCAGGGG TCTACCAGGT TTCTGGCCTC 
CACAACAAAG TGGCCACCAC CCCGGGGAGT CCCAGCCTGG GCCGGCACCC TGGGGCTCAC 
CAAGGCAACC TGGCCTCCGG TCTTCATAGC AATGCAATAG CCAGCCCTGG AAGCCCCAGC 
CTGGGCCGTC ACCTCGGAGG GTCTGGATCT GTGGTTCCCG GCAGCCCCTG CTTGGACCGG 
CATGTGGCCT ATGGTGGCTA TTCTACCCCG GAGGATCGGA GACCCACACT GTCCCGGCAG 
AGCAGTGCCT CTGGCTACCA GGCTCCTTCC ACGCCCTCCT TCCCTGTCTC CCCTGCCTAC 
TACCCTGGCC TGAGCAGCCC TGCCACCTCC CCGTCACCAG ACTCCGCAGC CTTCCGGCAA 
GGGAGCCCAA CACCAGCCTT GCCAGAGAAG CGAAGGATGT CAGTGGGAGA CCGGGCAGGC 
AGCCTCCCCA ACTATGCCAC CATCAATGGG AAGGTGTCTT CGCCTGTCGC CAGCGGCATG 
TCCAGTCCCA GTGGGGGCAG CACCGTCTCC TTCTCCCACA CTCTGCCCGA CTTCTCCAAG 
TACTCCATGC CAGACAACAG CCCGGAGACG CGGGCTAAAG TGAAGTTTGT CCAGGACACT 
TCTAAGTATT GGTACAAGCC TGAGATCTCC AGGGAGCAGG CCATCGCGCT CCTCAAGGAC 
CAGGAGCCGG GGGCCTTCAT CATCCGCGAC AGTCACTCCT TCCGAGGCGC GTACGGGCTG 
GCCATGAAGG TGTCTTCGCC ACCTCCAACC ATCATGCAGC AGAATAAAAA AGGAGACATG 
ACCCATGAGC TGGTCAGGCA TTTTCTGATA GAGACTGGCC CCAGAGGAGT CAAGCTCAAG 
GGCTGCCCCA ATGAGCCAAA CTTCGGATCG CTGTCTGCCC TGGTCTACCA GCACTCCATC 
ATCCCATTGG CCCTGCCTTG CAAGCTGGTC ATTCCAAACC GAGACCCCAC AGATGAATCG 
AAAGATAGCT CCGGCCCTGC CAACTCAACT GCAGACCTGC TGAAACAAGG GGCAGCCTGC 
AATGTGCTCT TCGTCAACTC TGTGGACATG GAGTCACTCA CTGGGCCACA GGCCATCTCT 
AAAGCCACAT CTGAGACGTT GGCTGCAGAC CCCACGCCAG CTGCCACCAT CGTTCACTTC 
AAAGTCTCTG CCCAGGGAAT CACTCTGACT GACAACCAGA GAAAGCTCTT TTTCAGACGC 
CACTACCCTC TCAACACTGT CACCTTCTGT GACCTGGATC CACAGGAAAG AAAGTGGATG 
AAAACAGAGG GTGGTGCCCC TGCTAAGCTC TTCGGCTTCG TGGCCCGGAA GCAGGGCAGC 
ACCACGGACA ACGCCTGCCA CCTCTTTGCT GAGCTTGACC CCAACCAGCC GGCCTCTGCC 
ATCGTCAACT TCGTCTCCAA GGTCATGCTG AATGCCGGCC AAAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860298.1
CDS	396..6086
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCINGNKQNTNSQSIGSISEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004809.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004809.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGCGTA CAACGCCAGG CTGCAGGGCA TCGGACACAG TGGCAGCTTC 
CCGCCCCCTC CTCTCCACCG GCTCCAATCT TCCTCCCTCT CTGGGGTACA GGCTCGGGAG 
AAGCAGCCTG CAGAGCCCCC AGCCCCTCTG CGGAGGCGGG CGGCCAGTGA TGGACAGTAT 
GAGAACCAGT CTCCAGAAGC CACATCCCCT CGTAGCCCTG GGGTTCGCTC CCCTGTCCAG 
TGTGTCTCCC CGGAGCTGGC TCTTACCATC GCTCTCAATC CTGGAGGGCG GCCCAAAGAG 
CCCCATTTGC ACAGCTACAA GGAGGCCTTC GAGGAGATGG AGGGAACCTC CCCGAGCAGC 
CCACCACCCA GTGGGGTGCG GTCCCCCCCG GGTCTGGCCA AGACACCCCT GTCTGCTCTG 
GGCCTGAAAC CTCACAACCC AGCGGACATC CTGTTGCACC CCACAGGAGT TACCAGAAGA 
CGGATCCAGC CAGATTCTCT TTCTCAAGAA ATAATCCCTG TTAATCTCTT TCTCTCTTTT 
TCCTTTTTTG CTCTTCCTCA TCTTCACCAC CTGCCCTTCT TTCCTGCCTC TCCTTCCTCC 
CACCTCTCTT CTCGTCTTCC CTCAGAGGAA GATGAGGGGA AGGTGGTGGT CAGGCTGTCA 
GAAGAGCCCC GGAGCTATGT GGAGTCTGTG GCACGGACAG CGGTGGCTGG ACCCCGAGCT 
CAGGACTCTG AGCCCAAGAG CTTTAGTGCT CCAGCCACCC AGGCCTATGG CCATGAGATA 
CCCCTGAGGA ACGGGACCCT GGGTGGCTCC TTTGTCTCCC CCAGCCCCCT CTCCACCAGC 
AGCCCCATCC TCAGTGCTGA CAGCACTTCA GTGGGGAGTT TCCCGTCGGG AGAGAGCAGT 
GACCAGGGTC CCCGGACGCC CACCCAGCCT CTGTTGGAGT CTGGCTTCCG CTCAGGCAGC 
CTGGGACAGC CCAGCCCGTC TGCCCAGAGA AACTACCAGA GCTCTTCTCC TCTCCCGACT 
GTGGGCAGTA GCTACAGCAG CCCCGACTAC TCACTTCAGC ATTTCAGCTC CTCTCCGGAA 
AGCCAGGCTC GAGCTCAGTT CAGTGTGGCT GGCGTCCACA CGGTGCCTGG GAGCCCTCAG 
GCGCGCCACA GAACAGTGGG CACCAACACT CCCCCTAGTC CTGGCTTCGG CTGGCGGGCC 
ATCAATCCCA GCATGGCTGC CCCCAGCAGT CCCAGTTTGA GCCATCACCA GATGATGGGT 
CCACCAGGCA CTGGCTTCCA TGGTAGCACT GTCTCCAGCC CCCAGAGCAG TGCAGCGACC 
ACCCCGGGGA GCCCCAGCCT GTGTCGGCAC CCAGCAGGGG TCTACCAGGT TTCTGGCCTC 
CACAACAAAG TGGCCACCAC CCCGGGGAGT CCCAGCCTGG GCCGGCACCC TGGGGCTCAC 
CAAGGCAACC TGGCCTCCGG TCTTCATAGC AATGCAATAG CCAGCCCTGG AAGCCCCAGC 
CTGGGCCGTC ACCTCGGAGG GTCTGGATCT GTGGTTCCCG GCAGCCCCTG CTTGGACCGG 
CATGTGGCCT ATGGTGGCTA TTCTACCCCG GAGGATCGGA GACCCACACT GTCCCGGCAG 
AGCAGTGCCT CTGGCTACCA GGCTCCTTCC ACGCCCTCCT TCCCTGTCTC CCCTGCCTAC 
TACCCTGGCC TGAGCAGCCC TGCCACCTCC CCGTCACCAG ACTCCGCAGC CTTCCGGCAA 
GGGAGCCCAA CACCAGCCTT GCCAGAGAAG CGAAGGATGT CAGTGGGAGA CCGGGCAGGC 
AGCCTCCCCA ACTATGCCAC CATCAATGGG AAGGTGTCTT CGCCTGTCGC CAGCGGCATG 
TCCAGTCCCA GTGGGGGCAG CACCGTCTCC TTCTCCCACA CTCTGCCCGA CTTCTCCAAG 
TACTCCATGC CAGACAACAG CCCGGAGACG CGGGCTAAAG TGAAGTTTGT CCAGGACACT 
TCTAAGTATT GGTACAAGCC TGAGATCTCC AGGGAGCAGG CCATCGCGCT CCTCAAGGAC 
CAGGAGCCGG GGGCCTTCAT CATCCGCGAC AGTCACTCCT TCCGAGGCGC GTACGGGCTG 
GCCATGAAGG TGTCTTCGCC ACCTCCAACC ATCATGCAGC AGAATAAAAA AGGAGACATG 
ACCCATGAGC TGGTCAGGCA TTTTCTGATA GAGACTGGCC CCAGAGGAGT CAAGCTCAAG 
GGCTGCCCCA ATGAGCCAAA CTTCGGATCG CTGTCTGCCC TGGTCTACCA GCACTCCATC 
ATCCCATTGG CCCTGCCTTG CAAGCTGGTC ATTCCAAACC GAGACCCCAC AGATGAATCG 
AAAGATAGCT CCGGCCCTGC CAACTCAACT GCAGACCTGC TGAAACAAGG GGCAGCCTGC 
AATGTGCTCT TCGTCAACTC TGTGGACATG GAGTCACTCA CTGGGCCACA GGCCATCTCT 
AAAGCCACAT CTGAGACGTT GGCTGCAGAC CCCACGCCAG CTGCCACCAT CGTTCACTTC 
AAAGTCTCTG CCCAGGGAAT CACTCTGACT GACAACCAGA GAAAGCTCTT TTTCAGACGC 
CACTACCCTC TCAACACTGT CACCTTCTGT GACCTGGATC CACAGGAAAG AAAGTGGATG 
AAAACAGAGG GTGGTGCCCC TGCTAAGCTC TTCGGCTTCG TGGCCCGGAA GCAGGGCAGC 
ACCACGGACA ACGCCTGCCA CCTCTTTGCT GAGCTTGACC CCAACCAGCC GGCCTCTGCC 
ATCGTCAACT TCGTCTCCAA GGTCATGCTG AATGCCGGCC AAAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453078.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308846.1 Homo sapiens tensin 1 (TNS1), transcript variant X11, mRNA.

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CloneID	OHu89842
Clone ID Related Accession (Same CDS sequence)	XM_024453078.1 , XM_017004811.1 , XM_017004812.1
Accession Version	XM_024453078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGCGTACAAC 
GCCAGGCTGC AGGGCATCGG ACACAGTGGC AGCTTCCCGC CCCCTCCTCT CCACCGGCTC 
CAATCTTCCT CCCTCTCTGG GGTACAGGCT CGGGAGAAGC AGCCTGCAGA GCCCCCAGCC 
CCTCTGCGGA GGCGGGCGGC CAGTGATGGA CAGTATGAGA ACCAGTCTCC AGAAGCCACA 
TCCCCTCGTA GCCCTGGGGT TCGCTCCCCT GTCCAGTGTG TCTCCCCGGA GCTGGCTCTT 
ACCATCGCTC TCAATCCTGG AGGGCGGCCC AAAGAGCCCC ATTTGCACAG CTACAAGGAG 
GCCTTCGAGG AGATGGAGGG AACCTCCCCG AGCAGCCCAC CACCCAGTGG GGTGCGGTCC 
CCCCCGGGTC TGGCCAAGAC ACCCCTGTCT GCTCTGGGCC TGAAACCTCA CAACCCAGCG 
GACATCCTGT TGCACCCCAC AGGAGTTACC AGAAGACGGA TCCAGCCAGA TTCTCTTTCT 
CAAGAAATAA TCCCTGTTAA TCTCTTTCTC TCTTTTTCCT TTTTTGCTCT TCCTCATCTT 
CACCACCTGC CCTTCTTTCC TGCCTCTCCT TCCTCCCACC TCTCTTCTCG TCTTCCCTCA 
GAGGAAGATG AGGGGAAGGT GGTGGTCAGG CTGTCAGAAG AGCCCCGGAG CTATGTGGAG 
TCTGTGGCAC GGACAGCGGT GGCTGGACCC CGAGCTCAGG ACTCTGAGCC CAAGAGCTTT 
AGTGCTCCAG CCACCCAGGC CTATGGCCAT GAGATACCCC TGAGGAACGG GACCCTGGGT 
GGCTCCTTTG TCTCCCCCAG CCCCCTCTCC ACCAGCAGCC CCATCCTCAG TGCTGACAGC 
ACTTCAGTGG GGAGTTTCCC GTCGGGAGAG AGCAGTGACC AGGGTCCCCG GACGCCCACC 
CAGCCTCTGT TGGAGTCTGG CTTCCGCTCA GGCAGCCTGG GACAGCCCAG CCCGTCTGCC 
CAGAGAAACT ACCAGAGCTC TTCTCCTCTC CCGACTGTGG GCAGTAGCTA CAGCAGCCCC 
GACTACTCAC TTCAGCATTT CAGCTCCTCT CCGGAAAGCC AGGCTCGAGC TCAGTTCAGT 
GTGGCTGGCG TCCACACGGT GCCTGGGAGC CCTCAGGCGC GCCACAGAAC AGTGGGCACC 
AACACTCCCC CTAGTCCTGG CTTCGGCTGG CGGGCCATCA ATCCCAGCAT GGCTGCCCCC 
AGCAGTCCCA GTTTGAGCCA TCACCAGATG ATGGGTCCAC CAGGCACTGG CTTCCATGGT 
AGCACTGTCT CCAGCCCCCA GAGCAGTGCA GCGACCACCC CGGGGAGCCC CAGCCTGTGT 
CGGCACCCAG CAGGGGTCTA CCAGGTTTCT GGCCTCCACA ACAAAGTGGC CACCACCCCG 
GGGAGTCCCA GCCTGGGCCG GCACCCTGGG GCTCACCAAG GCAACCTGGC CTCCGGTCTT 
CATAGCAATG CAATAGCCAG CCCTGGAAGC CCCAGCCTGG GCCGTCACCT CGGAGGGTCT 
GGATCTGTGG TTCCCGGCAG CCCCTGCTTG GACCGGCATG TGGCCTATGG TGGCTATTCT 
ACCCCGGAGG ATCGGAGACC CACACTGTCC CGGCAGAGCA GTGCCTCTGG CTACCAGGCT 
CCTTCCACGC CCTCCTTCCC TGTCTCCCCT GCCTACTACC CTGGCCTGAG CAGCCCTGCC 
ACCTCCCCGT CACCAGACTC CGCAGCCTTC CGGCAAGGGA GCCCAACACC AGCCTTGCCA 
GAGAAGCGAA GGATGTCAGT GGGAGACCGG GCAGGCAGCC TCCCCAACTA TGCCACCATC 
AATGGGAAGG TGTCTTCGCC TGTCGCCAGC GGCATGTCCA GTCCCAGTGG GGGCAGCACC 
GTCTCCTTCT CCCACACTCT GCCCGACTTC TCCAAGTACT CCATGCCAGA CAACAGCCCG 
GAGACGCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCTA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGGG AGCAGGCCAT CGCGCTCCTC AAGGACCAGG AGCCGGGGGC CTTCATCATC 
CGCGACAGTC ACTCCTTCCG AGGCGCGTAC GGGCTGGCCA TGAAGGTGTC TTCGCCACCT 
CCAACCATCA TGCAGCAGAA TAAAAAAGGA GACATGACCC ATGAGCTGGT CAGGCATTTT 
CTGATAGAGA CTGGCCCCAG AGGAGTCAAG CTCAAGGGCT GCCCCAATGA GCCAAACTTC 
GGATCGCTGT CTGCCCTGGT CTACCAGCAC TCCATCATCC CATTGGCCCT GCCTTGCAAG 
CTGGTCATTC CAAACCGAGA CCCCACAGAT GAATCGAAAG ATAGCTCCGG CCCTGCCAAC 
TCAACTGCAG ACCTGCTGAA ACAAGGGGCA GCCTGCAATG TGCTCTTCGT CAACTCTGTG 
GACATGGAGT CACTCACTGG GCCACAGGCC ATCTCTAAAG CCACATCTGA GACGTTGGCT 
GCAGACCCCA CGCCAGCTGC CACCATCGTT CACTTCAAAG TCTCTGCCCA GGGAATCACT 
CTGACTGACA ACCAGAGAAA GCTCTTTTTC AGACGCCACT ACCCTCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA GGAAAGAAAG TGGATGAAAA CAGAGGGTGG TGCCCCTGCT 
AAGCTCTTCG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CGGACAACGC CTGCCACCTC 
TTTGCTGAGC TTGACCCCAA CCAGCCGGCC TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAATG CCGGCCAAAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308846.1
CDS	343..5769
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453078.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453078.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGCGTACAAC 
GCCAGGCTGC AGGGCATCGG ACACAGTGGC AGCTTCCCGC CCCCTCCTCT CCACCGGCTC 
CAATCTTCCT CCCTCTCTGG GGTACAGGCT CGGGAGAAGC AGCCTGCAGA GCCCCCAGCC 
CCTCTGCGGA GGCGGGCGGC CAGTGATGGA CAGTATGAGA ACCAGTCTCC AGAAGCCACA 
TCCCCTCGTA GCCCTGGGGT TCGCTCCCCT GTCCAGTGTG TCTCCCCGGA GCTGGCTCTT 
ACCATCGCTC TCAATCCTGG AGGGCGGCCC AAAGAGCCCC ATTTGCACAG CTACAAGGAG 
GCCTTCGAGG AGATGGAGGG AACCTCCCCG AGCAGCCCAC CACCCAGTGG GGTGCGGTCC 
CCCCCGGGTC TGGCCAAGAC ACCCCTGTCT GCTCTGGGCC TGAAACCTCA CAACCCAGCG 
GACATCCTGT TGCACCCCAC AGGAGTTACC AGAAGACGGA TCCAGCCAGA TTCTCTTTCT 
CAAGAAATAA TCCCTGTTAA TCTCTTTCTC TCTTTTTCCT TTTTTGCTCT TCCTCATCTT 
CACCACCTGC CCTTCTTTCC TGCCTCTCCT TCCTCCCACC TCTCTTCTCG TCTTCCCTCA 
GAGGAAGATG AGGGGAAGGT GGTGGTCAGG CTGTCAGAAG AGCCCCGGAG CTATGTGGAG 
TCTGTGGCAC GGACAGCGGT GGCTGGACCC CGAGCTCAGG ACTCTGAGCC CAAGAGCTTT 
AGTGCTCCAG CCACCCAGGC CTATGGCCAT GAGATACCCC TGAGGAACGG GACCCTGGGT 
GGCTCCTTTG TCTCCCCCAG CCCCCTCTCC ACCAGCAGCC CCATCCTCAG TGCTGACAGC 
ACTTCAGTGG GGAGTTTCCC GTCGGGAGAG AGCAGTGACC AGGGTCCCCG GACGCCCACC 
CAGCCTCTGT TGGAGTCTGG CTTCCGCTCA GGCAGCCTGG GACAGCCCAG CCCGTCTGCC 
CAGAGAAACT ACCAGAGCTC TTCTCCTCTC CCGACTGTGG GCAGTAGCTA CAGCAGCCCC 
GACTACTCAC TTCAGCATTT CAGCTCCTCT CCGGAAAGCC AGGCTCGAGC TCAGTTCAGT 
GTGGCTGGCG TCCACACGGT GCCTGGGAGC CCTCAGGCGC GCCACAGAAC AGTGGGCACC 
AACACTCCCC CTAGTCCTGG CTTCGGCTGG CGGGCCATCA ATCCCAGCAT GGCTGCCCCC 
AGCAGTCCCA GTTTGAGCCA TCACCAGATG ATGGGTCCAC CAGGCACTGG CTTCCATGGT 
AGCACTGTCT CCAGCCCCCA GAGCAGTGCA GCGACCACCC CGGGGAGCCC CAGCCTGTGT 
CGGCACCCAG CAGGGGTCTA CCAGGTTTCT GGCCTCCACA ACAAAGTGGC CACCACCCCG 
GGGAGTCCCA GCCTGGGCCG GCACCCTGGG GCTCACCAAG GCAACCTGGC CTCCGGTCTT 
CATAGCAATG CAATAGCCAG CCCTGGAAGC CCCAGCCTGG GCCGTCACCT CGGAGGGTCT 
GGATCTGTGG TTCCCGGCAG CCCCTGCTTG GACCGGCATG TGGCCTATGG TGGCTATTCT 
ACCCCGGAGG ATCGGAGACC CACACTGTCC CGGCAGAGCA GTGCCTCTGG CTACCAGGCT 
CCTTCCACGC CCTCCTTCCC TGTCTCCCCT GCCTACTACC CTGGCCTGAG CAGCCCTGCC 
ACCTCCCCGT CACCAGACTC CGCAGCCTTC CGGCAAGGGA GCCCAACACC AGCCTTGCCA 
GAGAAGCGAA GGATGTCAGT GGGAGACCGG GCAGGCAGCC TCCCCAACTA TGCCACCATC 
AATGGGAAGG TGTCTTCGCC TGTCGCCAGC GGCATGTCCA GTCCCAGTGG GGGCAGCACC 
GTCTCCTTCT CCCACACTCT GCCCGACTTC TCCAAGTACT CCATGCCAGA CAACAGCCCG 
GAGACGCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCTA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGGG AGCAGGCCAT CGCGCTCCTC AAGGACCAGG AGCCGGGGGC CTTCATCATC 
CGCGACAGTC ACTCCTTCCG AGGCGCGTAC GGGCTGGCCA TGAAGGTGTC TTCGCCACCT 
CCAACCATCA TGCAGCAGAA TAAAAAAGGA GACATGACCC ATGAGCTGGT CAGGCATTTT 
CTGATAGAGA CTGGCCCCAG AGGAGTCAAG CTCAAGGGCT GCCCCAATGA GCCAAACTTC 
GGATCGCTGT CTGCCCTGGT CTACCAGCAC TCCATCATCC CATTGGCCCT GCCTTGCAAG 
CTGGTCATTC CAAACCGAGA CCCCACAGAT GAATCGAAAG ATAGCTCCGG CCCTGCCAAC 
TCAACTGCAG ACCTGCTGAA ACAAGGGGCA GCCTGCAATG TGCTCTTCGT CAACTCTGTG 
GACATGGAGT CACTCACTGG GCCACAGGCC ATCTCTAAAG CCACATCTGA GACGTTGGCT 
GCAGACCCCA CGCCAGCTGC CACCATCGTT CACTTCAAAG TCTCTGCCCA GGGAATCACT 
CTGACTGACA ACCAGAGAAA GCTCTTTTTC AGACGCCACT ACCCTCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA GGAAAGAAAG TGGATGAAAA CAGAGGGTGG TGCCCCTGCT 
AAGCTCTTCG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CGGACAACGC CTGCCACCTC 
TTTGCTGAGC TTGACCCCAA CCAGCCGGCC TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAATG CCGGCCAAAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004811.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860300.1 Homo sapiens tensin 1 (TNS1), transcript variant X17, mRNA.

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CloneID	OHu89842
Clone ID Related Accession (Same CDS sequence)	XM_024453078.1 , XM_017004811.1 , XM_017004812.1
Accession Version	XM_017004811.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGCGTACAAC 
GCCAGGCTGC AGGGCATCGG ACACAGTGGC AGCTTCCCGC CCCCTCCTCT CCACCGGCTC 
CAATCTTCCT CCCTCTCTGG GGTACAGGCT CGGGAGAAGC AGCCTGCAGA GCCCCCAGCC 
CCTCTGCGGA GGCGGGCGGC CAGTGATGGA CAGTATGAGA ACCAGTCTCC AGAAGCCACA 
TCCCCTCGTA GCCCTGGGGT TCGCTCCCCT GTCCAGTGTG TCTCCCCGGA GCTGGCTCTT 
ACCATCGCTC TCAATCCTGG AGGGCGGCCC AAAGAGCCCC ATTTGCACAG CTACAAGGAG 
GCCTTCGAGG AGATGGAGGG AACCTCCCCG AGCAGCCCAC CACCCAGTGG GGTGCGGTCC 
CCCCCGGGTC TGGCCAAGAC ACCCCTGTCT GCTCTGGGCC TGAAACCTCA CAACCCAGCG 
GACATCCTGT TGCACCCCAC AGGAGTTACC AGAAGACGGA TCCAGCCAGA TTCTCTTTCT 
CAAGAAATAA TCCCTGTTAA TCTCTTTCTC TCTTTTTCCT TTTTTGCTCT TCCTCATCTT 
CACCACCTGC CCTTCTTTCC TGCCTCTCCT TCCTCCCACC TCTCTTCTCG TCTTCCCTCA 
GAGGAAGATG AGGGGAAGGT GGTGGTCAGG CTGTCAGAAG AGCCCCGGAG CTATGTGGAG 
TCTGTGGCAC GGACAGCGGT GGCTGGACCC CGAGCTCAGG ACTCTGAGCC CAAGAGCTTT 
AGTGCTCCAG CCACCCAGGC CTATGGCCAT GAGATACCCC TGAGGAACGG GACCCTGGGT 
GGCTCCTTTG TCTCCCCCAG CCCCCTCTCC ACCAGCAGCC CCATCCTCAG TGCTGACAGC 
ACTTCAGTGG GGAGTTTCCC GTCGGGAGAG AGCAGTGACC AGGGTCCCCG GACGCCCACC 
CAGCCTCTGT TGGAGTCTGG CTTCCGCTCA GGCAGCCTGG GACAGCCCAG CCCGTCTGCC 
CAGAGAAACT ACCAGAGCTC TTCTCCTCTC CCGACTGTGG GCAGTAGCTA CAGCAGCCCC 
GACTACTCAC TTCAGCATTT CAGCTCCTCT CCGGAAAGCC AGGCTCGAGC TCAGTTCAGT 
GTGGCTGGCG TCCACACGGT GCCTGGGAGC CCTCAGGCGC GCCACAGAAC AGTGGGCACC 
AACACTCCCC CTAGTCCTGG CTTCGGCTGG CGGGCCATCA ATCCCAGCAT GGCTGCCCCC 
AGCAGTCCCA GTTTGAGCCA TCACCAGATG ATGGGTCCAC CAGGCACTGG CTTCCATGGT 
AGCACTGTCT CCAGCCCCCA GAGCAGTGCA GCGACCACCC CGGGGAGCCC CAGCCTGTGT 
CGGCACCCAG CAGGGGTCTA CCAGGTTTCT GGCCTCCACA ACAAAGTGGC CACCACCCCG 
GGGAGTCCCA GCCTGGGCCG GCACCCTGGG GCTCACCAAG GCAACCTGGC CTCCGGTCTT 
CATAGCAATG CAATAGCCAG CCCTGGAAGC CCCAGCCTGG GCCGTCACCT CGGAGGGTCT 
GGATCTGTGG TTCCCGGCAG CCCCTGCTTG GACCGGCATG TGGCCTATGG TGGCTATTCT 
ACCCCGGAGG ATCGGAGACC CACACTGTCC CGGCAGAGCA GTGCCTCTGG CTACCAGGCT 
CCTTCCACGC CCTCCTTCCC TGTCTCCCCT GCCTACTACC CTGGCCTGAG CAGCCCTGCC 
ACCTCCCCGT CACCAGACTC CGCAGCCTTC CGGCAAGGGA GCCCAACACC AGCCTTGCCA 
GAGAAGCGAA GGATGTCAGT GGGAGACCGG GCAGGCAGCC TCCCCAACTA TGCCACCATC 
AATGGGAAGG TGTCTTCGCC TGTCGCCAGC GGCATGTCCA GTCCCAGTGG GGGCAGCACC 
GTCTCCTTCT CCCACACTCT GCCCGACTTC TCCAAGTACT CCATGCCAGA CAACAGCCCG 
GAGACGCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCTA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGGG AGCAGGCCAT CGCGCTCCTC AAGGACCAGG AGCCGGGGGC CTTCATCATC 
CGCGACAGTC ACTCCTTCCG AGGCGCGTAC GGGCTGGCCA TGAAGGTGTC TTCGCCACCT 
CCAACCATCA TGCAGCAGAA TAAAAAAGGA GACATGACCC ATGAGCTGGT CAGGCATTTT 
CTGATAGAGA CTGGCCCCAG AGGAGTCAAG CTCAAGGGCT GCCCCAATGA GCCAAACTTC 
GGATCGCTGT CTGCCCTGGT CTACCAGCAC TCCATCATCC CATTGGCCCT GCCTTGCAAG 
CTGGTCATTC CAAACCGAGA CCCCACAGAT GAATCGAAAG ATAGCTCCGG CCCTGCCAAC 
TCAACTGCAG ACCTGCTGAA ACAAGGGGCA GCCTGCAATG TGCTCTTCGT CAACTCTGTG 
GACATGGAGT CACTCACTGG GCCACAGGCC ATCTCTAAAG CCACATCTGA GACGTTGGCT 
GCAGACCCCA CGCCAGCTGC CACCATCGTT CACTTCAAAG TCTCTGCCCA GGGAATCACT 
CTGACTGACA ACCAGAGAAA GCTCTTTTTC AGACGCCACT ACCCTCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA GGAAAGAAAG TGGATGAAAA CAGAGGGTGG TGCCCCTGCT 
AAGCTCTTCG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CGGACAACGC CTGCCACCTC 
TTTGCTGAGC TTGACCCCAA CCAGCCGGCC TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAATG CCGGCCAAAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860300.1
CDS	406..5832
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004811.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004811.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGCGTACAAC 
GCCAGGCTGC AGGGCATCGG ACACAGTGGC AGCTTCCCGC CCCCTCCTCT CCACCGGCTC 
CAATCTTCCT CCCTCTCTGG GGTACAGGCT CGGGAGAAGC AGCCTGCAGA GCCCCCAGCC 
CCTCTGCGGA GGCGGGCGGC CAGTGATGGA CAGTATGAGA ACCAGTCTCC AGAAGCCACA 
TCCCCTCGTA GCCCTGGGGT TCGCTCCCCT GTCCAGTGTG TCTCCCCGGA GCTGGCTCTT 
ACCATCGCTC TCAATCCTGG AGGGCGGCCC AAAGAGCCCC ATTTGCACAG CTACAAGGAG 
GCCTTCGAGG AGATGGAGGG AACCTCCCCG AGCAGCCCAC CACCCAGTGG GGTGCGGTCC 
CCCCCGGGTC TGGCCAAGAC ACCCCTGTCT GCTCTGGGCC TGAAACCTCA CAACCCAGCG 
GACATCCTGT TGCACCCCAC AGGAGTTACC AGAAGACGGA TCCAGCCAGA TTCTCTTTCT 
CAAGAAATAA TCCCTGTTAA TCTCTTTCTC TCTTTTTCCT TTTTTGCTCT TCCTCATCTT 
CACCACCTGC CCTTCTTTCC TGCCTCTCCT TCCTCCCACC TCTCTTCTCG TCTTCCCTCA 
GAGGAAGATG AGGGGAAGGT GGTGGTCAGG CTGTCAGAAG AGCCCCGGAG CTATGTGGAG 
TCTGTGGCAC GGACAGCGGT GGCTGGACCC CGAGCTCAGG ACTCTGAGCC CAAGAGCTTT 
AGTGCTCCAG CCACCCAGGC CTATGGCCAT GAGATACCCC TGAGGAACGG GACCCTGGGT 
GGCTCCTTTG TCTCCCCCAG CCCCCTCTCC ACCAGCAGCC CCATCCTCAG TGCTGACAGC 
ACTTCAGTGG GGAGTTTCCC GTCGGGAGAG AGCAGTGACC AGGGTCCCCG GACGCCCACC 
CAGCCTCTGT TGGAGTCTGG CTTCCGCTCA GGCAGCCTGG GACAGCCCAG CCCGTCTGCC 
CAGAGAAACT ACCAGAGCTC TTCTCCTCTC CCGACTGTGG GCAGTAGCTA CAGCAGCCCC 
GACTACTCAC TTCAGCATTT CAGCTCCTCT CCGGAAAGCC AGGCTCGAGC TCAGTTCAGT 
GTGGCTGGCG TCCACACGGT GCCTGGGAGC CCTCAGGCGC GCCACAGAAC AGTGGGCACC 
AACACTCCCC CTAGTCCTGG CTTCGGCTGG CGGGCCATCA ATCCCAGCAT GGCTGCCCCC 
AGCAGTCCCA GTTTGAGCCA TCACCAGATG ATGGGTCCAC CAGGCACTGG CTTCCATGGT 
AGCACTGTCT CCAGCCCCCA GAGCAGTGCA GCGACCACCC CGGGGAGCCC CAGCCTGTGT 
CGGCACCCAG CAGGGGTCTA CCAGGTTTCT GGCCTCCACA ACAAAGTGGC CACCACCCCG 
GGGAGTCCCA GCCTGGGCCG GCACCCTGGG GCTCACCAAG GCAACCTGGC CTCCGGTCTT 
CATAGCAATG CAATAGCCAG CCCTGGAAGC CCCAGCCTGG GCCGTCACCT CGGAGGGTCT 
GGATCTGTGG TTCCCGGCAG CCCCTGCTTG GACCGGCATG TGGCCTATGG TGGCTATTCT 
ACCCCGGAGG ATCGGAGACC CACACTGTCC CGGCAGAGCA GTGCCTCTGG CTACCAGGCT 
CCTTCCACGC CCTCCTTCCC TGTCTCCCCT GCCTACTACC CTGGCCTGAG CAGCCCTGCC 
ACCTCCCCGT CACCAGACTC CGCAGCCTTC CGGCAAGGGA GCCCAACACC AGCCTTGCCA 
GAGAAGCGAA GGATGTCAGT GGGAGACCGG GCAGGCAGCC TCCCCAACTA TGCCACCATC 
AATGGGAAGG TGTCTTCGCC TGTCGCCAGC GGCATGTCCA GTCCCAGTGG GGGCAGCACC 
GTCTCCTTCT CCCACACTCT GCCCGACTTC TCCAAGTACT CCATGCCAGA CAACAGCCCG 
GAGACGCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCTA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGGG AGCAGGCCAT CGCGCTCCTC AAGGACCAGG AGCCGGGGGC CTTCATCATC 
CGCGACAGTC ACTCCTTCCG AGGCGCGTAC GGGCTGGCCA TGAAGGTGTC TTCGCCACCT 
CCAACCATCA TGCAGCAGAA TAAAAAAGGA GACATGACCC ATGAGCTGGT CAGGCATTTT 
CTGATAGAGA CTGGCCCCAG AGGAGTCAAG CTCAAGGGCT GCCCCAATGA GCCAAACTTC 
GGATCGCTGT CTGCCCTGGT CTACCAGCAC TCCATCATCC CATTGGCCCT GCCTTGCAAG 
CTGGTCATTC CAAACCGAGA CCCCACAGAT GAATCGAAAG ATAGCTCCGG CCCTGCCAAC 
TCAACTGCAG ACCTGCTGAA ACAAGGGGCA GCCTGCAATG TGCTCTTCGT CAACTCTGTG 
GACATGGAGT CACTCACTGG GCCACAGGCC ATCTCTAAAG CCACATCTGA GACGTTGGCT 
GCAGACCCCA CGCCAGCTGC CACCATCGTT CACTTCAAAG TCTCTGCCCA GGGAATCACT 
CTGACTGACA ACCAGAGAAA GCTCTTTTTC AGACGCCACT ACCCTCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA GGAAAGAAAG TGGATGAAAA CAGAGGGTGG TGCCCCTGCT 
AAGCTCTTCG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CGGACAACGC CTGCCACCTC 
TTTGCTGAGC TTGACCCCAA CCAGCCGGCC TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAATG CCGGCCAAAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004812.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860301.1 Homo sapiens tensin 1 (TNS1), transcript variant X18, mRNA.

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CloneID	OHu89842
Clone ID Related Accession (Same CDS sequence)	XM_024453078.1 , XM_017004811.1 , XM_017004812.1
Accession Version	XM_017004812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGCGTACAAC 
GCCAGGCTGC AGGGCATCGG ACACAGTGGC AGCTTCCCGC CCCCTCCTCT CCACCGGCTC 
CAATCTTCCT CCCTCTCTGG GGTACAGGCT CGGGAGAAGC AGCCTGCAGA GCCCCCAGCC 
CCTCTGCGGA GGCGGGCGGC CAGTGATGGA CAGTATGAGA ACCAGTCTCC AGAAGCCACA 
TCCCCTCGTA GCCCTGGGGT TCGCTCCCCT GTCCAGTGTG TCTCCCCGGA GCTGGCTCTT 
ACCATCGCTC TCAATCCTGG AGGGCGGCCC AAAGAGCCCC ATTTGCACAG CTACAAGGAG 
GCCTTCGAGG AGATGGAGGG AACCTCCCCG AGCAGCCCAC CACCCAGTGG GGTGCGGTCC 
CCCCCGGGTC TGGCCAAGAC ACCCCTGTCT GCTCTGGGCC TGAAACCTCA CAACCCAGCG 
GACATCCTGT TGCACCCCAC AGGAGTTACC AGAAGACGGA TCCAGCCAGA TTCTCTTTCT 
CAAGAAATAA TCCCTGTTAA TCTCTTTCTC TCTTTTTCCT TTTTTGCTCT TCCTCATCTT 
CACCACCTGC CCTTCTTTCC TGCCTCTCCT TCCTCCCACC TCTCTTCTCG TCTTCCCTCA 
GAGGAAGATG AGGGGAAGGT GGTGGTCAGG CTGTCAGAAG AGCCCCGGAG CTATGTGGAG 
TCTGTGGCAC GGACAGCGGT GGCTGGACCC CGAGCTCAGG ACTCTGAGCC CAAGAGCTTT 
AGTGCTCCAG CCACCCAGGC CTATGGCCAT GAGATACCCC TGAGGAACGG GACCCTGGGT 
GGCTCCTTTG TCTCCCCCAG CCCCCTCTCC ACCAGCAGCC CCATCCTCAG TGCTGACAGC 
ACTTCAGTGG GGAGTTTCCC GTCGGGAGAG AGCAGTGACC AGGGTCCCCG GACGCCCACC 
CAGCCTCTGT TGGAGTCTGG CTTCCGCTCA GGCAGCCTGG GACAGCCCAG CCCGTCTGCC 
CAGAGAAACT ACCAGAGCTC TTCTCCTCTC CCGACTGTGG GCAGTAGCTA CAGCAGCCCC 
GACTACTCAC TTCAGCATTT CAGCTCCTCT CCGGAAAGCC AGGCTCGAGC TCAGTTCAGT 
GTGGCTGGCG TCCACACGGT GCCTGGGAGC CCTCAGGCGC GCCACAGAAC AGTGGGCACC 
AACACTCCCC CTAGTCCTGG CTTCGGCTGG CGGGCCATCA ATCCCAGCAT GGCTGCCCCC 
AGCAGTCCCA GTTTGAGCCA TCACCAGATG ATGGGTCCAC CAGGCACTGG CTTCCATGGT 
AGCACTGTCT CCAGCCCCCA GAGCAGTGCA GCGACCACCC CGGGGAGCCC CAGCCTGTGT 
CGGCACCCAG CAGGGGTCTA CCAGGTTTCT GGCCTCCACA ACAAAGTGGC CACCACCCCG 
GGGAGTCCCA GCCTGGGCCG GCACCCTGGG GCTCACCAAG GCAACCTGGC CTCCGGTCTT 
CATAGCAATG CAATAGCCAG CCCTGGAAGC CCCAGCCTGG GCCGTCACCT CGGAGGGTCT 
GGATCTGTGG TTCCCGGCAG CCCCTGCTTG GACCGGCATG TGGCCTATGG TGGCTATTCT 
ACCCCGGAGG ATCGGAGACC CACACTGTCC CGGCAGAGCA GTGCCTCTGG CTACCAGGCT 
CCTTCCACGC CCTCCTTCCC TGTCTCCCCT GCCTACTACC CTGGCCTGAG CAGCCCTGCC 
ACCTCCCCGT CACCAGACTC CGCAGCCTTC CGGCAAGGGA GCCCAACACC AGCCTTGCCA 
GAGAAGCGAA GGATGTCAGT GGGAGACCGG GCAGGCAGCC TCCCCAACTA TGCCACCATC 
AATGGGAAGG TGTCTTCGCC TGTCGCCAGC GGCATGTCCA GTCCCAGTGG GGGCAGCACC 
GTCTCCTTCT CCCACACTCT GCCCGACTTC TCCAAGTACT CCATGCCAGA CAACAGCCCG 
GAGACGCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCTA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGGG AGCAGGCCAT CGCGCTCCTC AAGGACCAGG AGCCGGGGGC CTTCATCATC 
CGCGACAGTC ACTCCTTCCG AGGCGCGTAC GGGCTGGCCA TGAAGGTGTC TTCGCCACCT 
CCAACCATCA TGCAGCAGAA TAAAAAAGGA GACATGACCC ATGAGCTGGT CAGGCATTTT 
CTGATAGAGA CTGGCCCCAG AGGAGTCAAG CTCAAGGGCT GCCCCAATGA GCCAAACTTC 
GGATCGCTGT CTGCCCTGGT CTACCAGCAC TCCATCATCC CATTGGCCCT GCCTTGCAAG 
CTGGTCATTC CAAACCGAGA CCCCACAGAT GAATCGAAAG ATAGCTCCGG CCCTGCCAAC 
TCAACTGCAG ACCTGCTGAA ACAAGGGGCA GCCTGCAATG TGCTCTTCGT CAACTCTGTG 
GACATGGAGT CACTCACTGG GCCACAGGCC ATCTCTAAAG CCACATCTGA GACGTTGGCT 
GCAGACCCCA CGCCAGCTGC CACCATCGTT CACTTCAAAG TCTCTGCCCA GGGAATCACT 
CTGACTGACA ACCAGAGAAA GCTCTTTTTC AGACGCCACT ACCCTCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA GGAAAGAAAG TGGATGAAAA CAGAGGGTGG TGCCCCTGCT 
AAGCTCTTCG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CGGACAACGC CTGCCACCTC 
TTTGCTGAGC TTGACCCCAA CCAGCCGGCC TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAATG CCGGCCAAAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860301.1
CDS	356..5782
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004812.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004812.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGCGTACAAC 
GCCAGGCTGC AGGGCATCGG ACACAGTGGC AGCTTCCCGC CCCCTCCTCT CCACCGGCTC 
CAATCTTCCT CCCTCTCTGG GGTACAGGCT CGGGAGAAGC AGCCTGCAGA GCCCCCAGCC 
CCTCTGCGGA GGCGGGCGGC CAGTGATGGA CAGTATGAGA ACCAGTCTCC AGAAGCCACA 
TCCCCTCGTA GCCCTGGGGT TCGCTCCCCT GTCCAGTGTG TCTCCCCGGA GCTGGCTCTT 
ACCATCGCTC TCAATCCTGG AGGGCGGCCC AAAGAGCCCC ATTTGCACAG CTACAAGGAG 
GCCTTCGAGG AGATGGAGGG AACCTCCCCG AGCAGCCCAC CACCCAGTGG GGTGCGGTCC 
CCCCCGGGTC TGGCCAAGAC ACCCCTGTCT GCTCTGGGCC TGAAACCTCA CAACCCAGCG 
GACATCCTGT TGCACCCCAC AGGAGTTACC AGAAGACGGA TCCAGCCAGA TTCTCTTTCT 
CAAGAAATAA TCCCTGTTAA TCTCTTTCTC TCTTTTTCCT TTTTTGCTCT TCCTCATCTT 
CACCACCTGC CCTTCTTTCC TGCCTCTCCT TCCTCCCACC TCTCTTCTCG TCTTCCCTCA 
GAGGAAGATG AGGGGAAGGT GGTGGTCAGG CTGTCAGAAG AGCCCCGGAG CTATGTGGAG 
TCTGTGGCAC GGACAGCGGT GGCTGGACCC CGAGCTCAGG ACTCTGAGCC CAAGAGCTTT 
AGTGCTCCAG CCACCCAGGC CTATGGCCAT GAGATACCCC TGAGGAACGG GACCCTGGGT 
GGCTCCTTTG TCTCCCCCAG CCCCCTCTCC ACCAGCAGCC CCATCCTCAG TGCTGACAGC 
ACTTCAGTGG GGAGTTTCCC GTCGGGAGAG AGCAGTGACC AGGGTCCCCG GACGCCCACC 
CAGCCTCTGT TGGAGTCTGG CTTCCGCTCA GGCAGCCTGG GACAGCCCAG CCCGTCTGCC 
CAGAGAAACT ACCAGAGCTC TTCTCCTCTC CCGACTGTGG GCAGTAGCTA CAGCAGCCCC 
GACTACTCAC TTCAGCATTT CAGCTCCTCT CCGGAAAGCC AGGCTCGAGC TCAGTTCAGT 
GTGGCTGGCG TCCACACGGT GCCTGGGAGC CCTCAGGCGC GCCACAGAAC AGTGGGCACC 
AACACTCCCC CTAGTCCTGG CTTCGGCTGG CGGGCCATCA ATCCCAGCAT GGCTGCCCCC 
AGCAGTCCCA GTTTGAGCCA TCACCAGATG ATGGGTCCAC CAGGCACTGG CTTCCATGGT 
AGCACTGTCT CCAGCCCCCA GAGCAGTGCA GCGACCACCC CGGGGAGCCC CAGCCTGTGT 
CGGCACCCAG CAGGGGTCTA CCAGGTTTCT GGCCTCCACA ACAAAGTGGC CACCACCCCG 
GGGAGTCCCA GCCTGGGCCG GCACCCTGGG GCTCACCAAG GCAACCTGGC CTCCGGTCTT 
CATAGCAATG CAATAGCCAG CCCTGGAAGC CCCAGCCTGG GCCGTCACCT CGGAGGGTCT 
GGATCTGTGG TTCCCGGCAG CCCCTGCTTG GACCGGCATG TGGCCTATGG TGGCTATTCT 
ACCCCGGAGG ATCGGAGACC CACACTGTCC CGGCAGAGCA GTGCCTCTGG CTACCAGGCT 
CCTTCCACGC CCTCCTTCCC TGTCTCCCCT GCCTACTACC CTGGCCTGAG CAGCCCTGCC 
ACCTCCCCGT CACCAGACTC CGCAGCCTTC CGGCAAGGGA GCCCAACACC AGCCTTGCCA 
GAGAAGCGAA GGATGTCAGT GGGAGACCGG GCAGGCAGCC TCCCCAACTA TGCCACCATC 
AATGGGAAGG TGTCTTCGCC TGTCGCCAGC GGCATGTCCA GTCCCAGTGG GGGCAGCACC 
GTCTCCTTCT CCCACACTCT GCCCGACTTC TCCAAGTACT CCATGCCAGA CAACAGCCCG 
GAGACGCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCTA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGGG AGCAGGCCAT CGCGCTCCTC AAGGACCAGG AGCCGGGGGC CTTCATCATC 
CGCGACAGTC ACTCCTTCCG AGGCGCGTAC GGGCTGGCCA TGAAGGTGTC TTCGCCACCT 
CCAACCATCA TGCAGCAGAA TAAAAAAGGA GACATGACCC ATGAGCTGGT CAGGCATTTT 
CTGATAGAGA CTGGCCCCAG AGGAGTCAAG CTCAAGGGCT GCCCCAATGA GCCAAACTTC 
GGATCGCTGT CTGCCCTGGT CTACCAGCAC TCCATCATCC CATTGGCCCT GCCTTGCAAG 
CTGGTCATTC CAAACCGAGA CCCCACAGAT GAATCGAAAG ATAGCTCCGG CCCTGCCAAC 
TCAACTGCAG ACCTGCTGAA ACAAGGGGCA GCCTGCAATG TGCTCTTCGT CAACTCTGTG 
GACATGGAGT CACTCACTGG GCCACAGGCC ATCTCTAAAG CCACATCTGA GACGTTGGCT 
GCAGACCCCA CGCCAGCTGC CACCATCGTT CACTTCAAAG TCTCTGCCCA GGGAATCACT 
CTGACTGACA ACCAGAGAAA GCTCTTTTTC AGACGCCACT ACCCTCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA GGAAAGAAAG TGGATGAAAA CAGAGGGTGG TGCCCCTGCT 
AAGCTCTTCG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CGGACAACGC CTGCCACCTC 
TTTGCTGAGC TTGACCCCAA CCAGCCGGCC TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAATG CCGGCCAAAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004813.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860302.1 Homo sapiens tensin 1 (TNS1), transcript variant X17, mRNA.

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CloneID	OHu89843
Clone ID Related Accession (Same CDS sequence)	XM_017004813.1 , XM_017004813.2
Accession Version	XM_017004813.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGGC CCTTTGCTGC ACCCTACTGC CCTCCTGGGT TCTCCCCCTG CTTCCGTGGG 
CAAAGGCTAT CCTCGGGGCG ACTGGCCGTC CATGCTGATA CCTCTATGGG CATTCAAGGC 
AATGGCTACT CTCCTGGCAT TCTGCCTCTA ATCCAGAGTG GGAACTCCAG ACATCGAAGC 
AGGGAGGTGG TGGGACACAC GCAGGGGCCA CTAGATGGGA GCCTGTATGC TAAGGTGAAG 
AAGAAAGACT CCCTGCACGG CAGCACCGGG GCTGTTAATG CCACACGTCC TACACTGTCG 
GCCACCCCCA ACCACGTGGA ACACACGCTT TCTGTGAGCA GCGACTCGGG CAACTCCACA 
GCCTCCACCA AGACCGACAA GACCGACGAG CCTGTCCCCG GGGCCTCCAG TGCCACTGCT 
GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG GACCGCCTGC TGAGTGGCTT TGGCTTAGAG 
CGAGAGAAGC AAGGCGCCAT GTACCACACC CAGCACCTCA GGTCCCGCCC AGCAGGGGGC 
TCGGCTGTGC CCTCCTCTGG ACGCCACGTT GTCCCAGCCC AGGTTCATGT CAATGGTGGG 
GCGTTAGCAT CTGAGCGGGA GACAGACATC CTGGACGATG AATTGCCAAA CCAGGATGGT 
CACAGTGCGG GCAGCATGGG CACACTCTCT TCTCTGGACG GGGTCACCAA CACCAGTGAG 
GGGGGCTACC CAGAGGCCCT GTCCCCACTG ACCAACGGTC TGGACAAGTC CTACCCCATG 
GAGCCTATGG TCAATGGAGG AGGCTACCCC TACGAGTCTG CCAGCCGGGC GGGGCCTGCC 
CATGCTGGCC ACACGGCCCC CATGCGGCCC TCCTACTCTG CACAGGAGGG TTTAGCTGGC 
TACCAGAGGG AGGGGCCCCA CCCAGCCTGG CCACAGCCAG TGACCACCTC CCACTATGCC 
CATGACCCCA GCGGTATGTT CCGCTCTCAA TCCTTTTCGG AAGCTGAACC CCAGCTGCCC 
CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG GAGGCTGTGC AAAGGGGACT GAATTCGTGG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT CGCCCACCTC CACGCCAGCA GGAAAGAGCC 
CACTTGGAGA GTCTTGTAGC CAGCAGGCCC AGCCCTCAGC CATTGGCAGA GACCCCCATC 
CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC TCCCAGCAGG AGATTGAACA GTCCATCGAA 
ACACTCAATA TGCTGATGCT GGACCTGGAG CCAGCCTCCG CTGCTGCCCC ACTACACAAG 
TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT 
GGATCCTCCC GTCAGTCCCA TCCACTGACC CAGTCCAGAT CTGGCTATAT CCCCAGTGGG 
CATTCGTTGG GAACCCCTGA GCCAGCCCCA CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC 
AGGTCTTACT CACCTTATGA CTATCAGCCA TGTTTGGCTG GGCCTAACCA GGATTTCCAT 
TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT GCCTTCCTTC CGACCACCCA CAGCCCTCCA 
GGGCCTCAGC AACCCCCAGC CTCTCTCCCT GGCCTCACTG CTCAGCCTCT GCTCTCACCA 
AAGGAAGCGA CTTCAGACCC CTCCCGGACT CCAGAGGAGG AGCCATTGAA TTTAGAAGGG 
CTGGTGGCCC ACAGGGTAGC AGCGTACAAC GCCAGGCTGC AGGGCATCGG ACACAGTGGC 
AGCTTCCCGC CCCCTCCTCT CCACCGGCTC CAATCTTCCT CCCTCTCTGG GGTACAGGCT 
CGGGAGAAGC AGCCTGCAGA GCCCCCAGCC CCTCTGCGGA GGCGGGCGGC CAGTGATGGA 
CAGTATGAGA ACCAGTCTCC AGAAGCCACA TCCCCTCGTA GCCCTGGGGT TCGCTCCCCT 
GTCCAGTGTG TCTCCCCGGA GCTGGCTCTT ACCATCGCTC TCAATCCTGG AGGGCGGCCC 
AAAGAGCCCC ATTTGCACAG CTACAAGGAG GCCTTCGAGG AGATGGAGGG AACCTCCCCG 
AGCAGCCCAC CACCCAGTGG GGTGCGGTCC CCCCCGGGTC TGGCCAAGAC ACCCCTGTCT 
GCTCTGGGCC TGAAACCTCA CAACCCAGCG GACATCCTGT TGCACCCCAC AGGAGTTACC 
AGAAGACGGA TCCAGCCAGA TTCTCTTTCT CAAGAAATAA TCCCTGTTAA TCTCTTTCTC 
TCTTTTTCCT TTTTTGCTCT TCCTCATCTT CACCACCTGC CCTTCTTTCC TGCCTCTCCT 
TCCTCCCACC TCTCTTCTCG TCTTCCCTCA GAGGAAGATG AGGGGAAGGT GGTGGTCAGG 
CTGTCAGAAG AGCCCCGGAG CTATGTGGAG TCTGTGGCAC GGACAGCGGT GGCTGGACCC 
CGAGCTCAGG ACTCTGAGCC CAAGAGCTTT AGTGCTCCAG CCACCCAGGC CTATGGCCAT 
GAGATACCCC TGAGGAACGG GACCCTGGGT GGCTCCTTTG TCTCCCCCAG CCCCCTCTCC 
ACCAGCAGCC CCATCCTCAG TGCTGACAGC ACTTCAGTGG GGAGTTTCCC GTCGGGAGAG 
AGCAGTGACC AGGGTCCCCG GACGCCCACC CAGCCTCTGT TGGAGTCTGG CTTCCGCTCA 
GGCAGCCTGG GACAGCCCAG CCCGTCTGCC CAGAGAAACT ACCAGAGCTC TTCTCCTCTC 
CCGACTGTGG GCAGTAGCTA CAGCAGCCCC GACTACTCAC TTCAGCATTT CAGCTCCTCT 
CCGGAAAGCC AGGCTCGAGC TCAGTTCAGT GTGGCTGGCG TCCACACGGT GCCTGGGAGC 
CCTCAGGCGC GCCACAGAAC AGTGGGCACC AACACTCCCC CTAGTCCTGG CTTCGGCTGG 
CGGGCCATCA ATCCCAGCAT GGCTGCCCCC AGCAGTCCCA GTTTGAGCCA TCACCAGATG 
ATGGGTCCAC CAGGCACTGG CTTCCATGGT AGCACTGTCT CCAGCCCCCA GAGCAGTGCA 
GCGACCACCC CGGGGAGCCC CAGCCTGTGT CGGCACCCAG CAGGGGTCTA CCAGGTTTCT 
GGCCTCCACA ACAAAGTGGC CACCACCCCG GGGAGTCCCA GCCTGGGCCG GCACCCTGGG 
GCTCACCAAG GCAACCTGGC CTCCGGTCTT CATAGCAATG CAATAGCCAG CCCTGGAAGC 
CCCAGCCTGG GCCGTCACCT CGGAGGGTCT GGATCTGTGG TTCCCGGCAG CCCCTGCTTG 
GACCGGCATG TGGCCTATGG TGGCTATTCT ACCCCGGAGG ATCGGAGACC CACACTGTCC 
CGGCAGAGCA GTGCCTCTGG CTACCAGGCT CCTTCCACGC CCTCCTTCCC TGTCTCCCCT 
GCCTACTACC CTGGCCTGAG CAGCCCTGCC ACCTCCCCGT CACCAGACTC CGCAGCCTTC 
CGGCAAGGGA GCCCAACACC AGCCTTGCCA GAGAAGCGAA GGATGTCAGT GGGAGACCGG 
GCAGGCAGCC TCCCCAACTA TGCCACCATC AATGGGAAGG TGTCTTCGCC TGTCGCCAGC 
GGCATGTCCA GTCCCAGTGG GGGCAGCACC GTCTCCTTCT CCCACACTCT GCCCGACTTC 
TCCAAGTACT CCATGCCAGA CAACAGCCCG GAGACGCGGG CTAAAGTGAA GTTTGTCCAG 
GACACTTCTA AGTATTGGTA CAAGCCTGAG ATCTCCAGGG AGCAGGCCAT CGCGCTCCTC 
AAGGACCAGG AGCCGGGGGC CTTCATCATC CGCGACAGTC ACTCCTTCCG AGGCGCGTAC 
GGGCTGGCCA TGAAGGTGTC TTCGCCACCT CCAACCATCA TGCAGCAGAA TAAAAAAGGA 
GACATGACCC ATGAGCTGGT CAGGCATTTT CTGATAGAGA CTGGCCCCAG AGGAGTCAAG 
CTCAAGGGCT GCCCCAATGA GCCAAACTTC GGATCGCTGT CTGCCCTGGT CTACCAGCAC 
TCCATCATCC CATTGGCCCT GCCTTGCAAG CTGGTCATTC CAAACCGAGA CCCCACAGAT 
GAATCGAAAG ATAGCTCCGG CCCTGCCAAC TCAACTGCAG ACCTGCTGAA ACAAGGGGCA 
GCCTGCAATG TGCTCTTCGT CAACTCTGTG GACATGGAGT CACTCACTGG GCCACAGGCC 
ATCTCTAAAG CCACATCTGA GACGTTGGCT GCAGACCCCA CGCCAGCTGC CACCATCGTT 
CACTTCAAAG TCTCTGCCCA GGGAATCACT CTGACTGACA ACCAGAGAAA GCTCTTTTTC 
AGACGCCACT ACCCTCTCAA CACTGTCACC TTCTGTGACC TGGATCCACA GGAAAGAAAG 
TGGATGAAAA CAGAGGGTGG TGCCCCTGCT AAGCTCTTCG GCTTCGTGGC CCGGAAGCAG 
GGCAGCACCA CGGACAACGC CTGCCACCTC TTTGCTGAGC TTGACCCCAA CCAGCCGGCC 
TCTGCCATCG TCAACTTCGT CTCCAAGGTC ATGCTGAATG CCGGCCAAAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860302.1
CDS	138..4694
Translation	MVGPFAAPYCPPGFSPCFRGQRLSSGRLAVHADTSMGIQGNGYSPGILPLIQSGNSRHRSREVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004813.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004813.1

ORF Insert Sequence:

ATGGTGGGGC CCTTTGCTGC ACCCTACTGC CCTCCTGGGT TCTCCCCCTG CTTCCGTGGG 
CAAAGGCTAT CCTCGGGGCG ACTGGCCGTC CATGCTGATA CCTCTATGGG CATTCAAGGC 
AATGGCTACT CTCCTGGCAT TCTGCCTCTA ATCCAGAGTG GGAACTCCAG ACATCGAAGC 
AGGGAGGTGG TGGGACACAC GCAGGGGCCA CTAGATGGGA GCCTGTATGC TAAGGTGAAG 
AAGAAAGACT CCCTGCACGG CAGCACCGGG GCTGTTAATG CCACACGTCC TACACTGTCG 
GCCACCCCCA ACCACGTGGA ACACACGCTT TCTGTGAGCA GCGACTCGGG CAACTCCACA 
GCCTCCACCA AGACCGACAA GACCGACGAG CCTGTCCCCG GGGCCTCCAG TGCCACTGCT 
GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG GACCGCCTGC TGAGTGGCTT TGGCTTAGAG 
CGAGAGAAGC AAGGCGCCAT GTACCACACC CAGCACCTCA GGTCCCGCCC AGCAGGGGGC 
TCGGCTGTGC CCTCCTCTGG ACGCCACGTT GTCCCAGCCC AGGTTCATGT CAATGGTGGG 
GCGTTAGCAT CTGAGCGGGA GACAGACATC CTGGACGATG AATTGCCAAA CCAGGATGGT 
CACAGTGCGG GCAGCATGGG CACACTCTCT TCTCTGGACG GGGTCACCAA CACCAGTGAG 
GGGGGCTACC CAGAGGCCCT GTCCCCACTG ACCAACGGTC TGGACAAGTC CTACCCCATG 
GAGCCTATGG TCAATGGAGG AGGCTACCCC TACGAGTCTG CCAGCCGGGC GGGGCCTGCC 
CATGCTGGCC ACACGGCCCC CATGCGGCCC TCCTACTCTG CACAGGAGGG TTTAGCTGGC 
TACCAGAGGG AGGGGCCCCA CCCAGCCTGG CCACAGCCAG TGACCACCTC CCACTATGCC 
CATGACCCCA GCGGTATGTT CCGCTCTCAA TCCTTTTCGG AAGCTGAACC CCAGCTGCCC 
CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG GAGGCTGTGC AAAGGGGACT GAATTCGTGG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT CGCCCACCTC CACGCCAGCA GGAAAGAGCC 
CACTTGGAGA GTCTTGTAGC CAGCAGGCCC AGCCCTCAGC CATTGGCAGA GACCCCCATC 
CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC TCCCAGCAGG AGATTGAACA GTCCATCGAA 
ACACTCAATA TGCTGATGCT GGACCTGGAG CCAGCCTCCG CTGCTGCCCC ACTACACAAG 
TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT 
GGATCCTCCC GTCAGTCCCA TCCACTGACC CAGTCCAGAT CTGGCTATAT CCCCAGTGGG 
CATTCGTTGG GAACCCCTGA GCCAGCCCCA CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC 
AGGTCTTACT CACCTTATGA CTATCAGCCA TGTTTGGCTG GGCCTAACCA GGATTTCCAT 
TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT GCCTTCCTTC CGACCACCCA CAGCCCTCCA 
GGGCCTCAGC AACCCCCAGC CTCTCTCCCT GGCCTCACTG CTCAGCCTCT GCTCTCACCA 
AAGGAAGCGA CTTCAGACCC CTCCCGGACT CCAGAGGAGG AGCCATTGAA TTTAGAAGGG 
CTGGTGGCCC ACAGGGTAGC AGCGTACAAC GCCAGGCTGC AGGGCATCGG ACACAGTGGC 
AGCTTCCCGC CCCCTCCTCT CCACCGGCTC CAATCTTCCT CCCTCTCTGG GGTACAGGCT 
CGGGAGAAGC AGCCTGCAGA GCCCCCAGCC CCTCTGCGGA GGCGGGCGGC CAGTGATGGA 
CAGTATGAGA ACCAGTCTCC AGAAGCCACA TCCCCTCGTA GCCCTGGGGT TCGCTCCCCT 
GTCCAGTGTG TCTCCCCGGA GCTGGCTCTT ACCATCGCTC TCAATCCTGG AGGGCGGCCC 
AAAGAGCCCC ATTTGCACAG CTACAAGGAG GCCTTCGAGG AGATGGAGGG AACCTCCCCG 
AGCAGCCCAC CACCCAGTGG GGTGCGGTCC CCCCCGGGTC TGGCCAAGAC ACCCCTGTCT 
GCTCTGGGCC TGAAACCTCA CAACCCAGCG GACATCCTGT TGCACCCCAC AGGAGTTACC 
AGAAGACGGA TCCAGCCAGA TTCTCTTTCT CAAGAAATAA TCCCTGTTAA TCTCTTTCTC 
TCTTTTTCCT TTTTTGCTCT TCCTCATCTT CACCACCTGC CCTTCTTTCC TGCCTCTCCT 
TCCTCCCACC TCTCTTCTCG TCTTCCCTCA GAGGAAGATG AGGGGAAGGT GGTGGTCAGG 
CTGTCAGAAG AGCCCCGGAG CTATGTGGAG TCTGTGGCAC GGACAGCGGT GGCTGGACCC 
CGAGCTCAGG ACTCTGAGCC CAAGAGCTTT AGTGCTCCAG CCACCCAGGC CTATGGCCAT 
GAGATACCCC TGAGGAACGG GACCCTGGGT GGCTCCTTTG TCTCCCCCAG CCCCCTCTCC 
ACCAGCAGCC CCATCCTCAG TGCTGACAGC ACTTCAGTGG GGAGTTTCCC GTCGGGAGAG 
AGCAGTGACC AGGGTCCCCG GACGCCCACC CAGCCTCTGT TGGAGTCTGG CTTCCGCTCA 
GGCAGCCTGG GACAGCCCAG CCCGTCTGCC CAGAGAAACT ACCAGAGCTC TTCTCCTCTC 
CCGACTGTGG GCAGTAGCTA CAGCAGCCCC GACTACTCAC TTCAGCATTT CAGCTCCTCT 
CCGGAAAGCC AGGCTCGAGC TCAGTTCAGT GTGGCTGGCG TCCACACGGT GCCTGGGAGC 
CCTCAGGCGC GCCACAGAAC AGTGGGCACC AACACTCCCC CTAGTCCTGG CTTCGGCTGG 
CGGGCCATCA ATCCCAGCAT GGCTGCCCCC AGCAGTCCCA GTTTGAGCCA TCACCAGATG 
ATGGGTCCAC CAGGCACTGG CTTCCATGGT AGCACTGTCT CCAGCCCCCA GAGCAGTGCA 
GCGACCACCC CGGGGAGCCC CAGCCTGTGT CGGCACCCAG CAGGGGTCTA CCAGGTTTCT 
GGCCTCCACA ACAAAGTGGC CACCACCCCG GGGAGTCCCA GCCTGGGCCG GCACCCTGGG 
GCTCACCAAG GCAACCTGGC CTCCGGTCTT CATAGCAATG CAATAGCCAG CCCTGGAAGC 
CCCAGCCTGG GCCGTCACCT CGGAGGGTCT GGATCTGTGG TTCCCGGCAG CCCCTGCTTG 
GACCGGCATG TGGCCTATGG TGGCTATTCT ACCCCGGAGG ATCGGAGACC CACACTGTCC 
CGGCAGAGCA GTGCCTCTGG CTACCAGGCT CCTTCCACGC CCTCCTTCCC TGTCTCCCCT 
GCCTACTACC CTGGCCTGAG CAGCCCTGCC ACCTCCCCGT CACCAGACTC CGCAGCCTTC 
CGGCAAGGGA GCCCAACACC AGCCTTGCCA GAGAAGCGAA GGATGTCAGT GGGAGACCGG 
GCAGGCAGCC TCCCCAACTA TGCCACCATC AATGGGAAGG TGTCTTCGCC TGTCGCCAGC 
GGCATGTCCA GTCCCAGTGG GGGCAGCACC GTCTCCTTCT CCCACACTCT GCCCGACTTC 
TCCAAGTACT CCATGCCAGA CAACAGCCCG GAGACGCGGG CTAAAGTGAA GTTTGTCCAG 
GACACTTCTA AGTATTGGTA CAAGCCTGAG ATCTCCAGGG AGCAGGCCAT CGCGCTCCTC 
AAGGACCAGG AGCCGGGGGC CTTCATCATC CGCGACAGTC ACTCCTTCCG AGGCGCGTAC 
GGGCTGGCCA TGAAGGTGTC TTCGCCACCT CCAACCATCA TGCAGCAGAA TAAAAAAGGA 
GACATGACCC ATGAGCTGGT CAGGCATTTT CTGATAGAGA CTGGCCCCAG AGGAGTCAAG 
CTCAAGGGCT GCCCCAATGA GCCAAACTTC GGATCGCTGT CTGCCCTGGT CTACCAGCAC 
TCCATCATCC CATTGGCCCT GCCTTGCAAG CTGGTCATTC CAAACCGAGA CCCCACAGAT 
GAATCGAAAG ATAGCTCCGG CCCTGCCAAC TCAACTGCAG ACCTGCTGAA ACAAGGGGCA 
GCCTGCAATG TGCTCTTCGT CAACTCTGTG GACATGGAGT CACTCACTGG GCCACAGGCC 
ATCTCTAAAG CCACATCTGA GACGTTGGCT GCAGACCCCA CGCCAGCTGC CACCATCGTT 
CACTTCAAAG TCTCTGCCCA GGGAATCACT CTGACTGACA ACCAGAGAAA GCTCTTTTTC 
AGACGCCACT ACCCTCTCAA CACTGTCACC TTCTGTGACC TGGATCCACA GGAAAGAAAG 
TGGATGAAAA CAGAGGGTGG TGCCCCTGCT AAGCTCTTCG GCTTCGTGGC CCGGAAGCAG 
GGCAGCACCA CGGACAACGC CTGCCACCTC TTTGCTGAGC TTGACCCCAA CCAGCCGGCC 
TCTGCCATCG TCAACTTCGT CTCCAAGGTC ATGCTGAATG CCGGCCAAAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004813.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860302.1 Homo sapiens tensin 1 (TNS1), transcript variant X20, mRNA.

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CloneID	OHu89843
Clone ID Related Accession (Same CDS sequence)	XM_017004813.1 , XM_017004813.2
Accession Version	XM_017004813.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017004813.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGGGC CCTTTGCTGC ACCCTACTGC CCTCCTGGGT TCTCCCCCTG CTTCCGTGGG 
CAAAGGCTAT CCTCGGGGCG ACTGGCCGTC CATGCTGATA CCTCTATGGG CATTCAAGGC 
AATGGCTACT CTCCTGGCAT TCTGCCTCTA ATCCAGAGTG GGAACTCCAG ACATCGAAGC 
AGGGAGGTGG TGGGACACAC GCAGGGGCCA CTAGATGGGA GCCTGTATGC TAAGGTGAAG 
AAGAAAGACT CCCTGCACGG CAGCACCGGG GCTGTTAATG CCACACGTCC TACACTGTCG 
GCCACCCCCA ACCACGTGGA ACACACGCTT TCTGTGAGCA GCGACTCGGG CAACTCCACA 
GCCTCCACCA AGACCGACAA GACCGACGAG CCTGTCCCCG GGGCCTCCAG TGCCACTGCT 
GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG GACCGCCTGC TGAGTGGCTT TGGCTTAGAG 
CGAGAGAAGC AAGGCGCCAT GTACCACACC CAGCACCTCA GGTCCCGCCC AGCAGGGGGC 
TCGGCTGTGC CCTCCTCTGG ACGCCACGTT GTCCCAGCCC AGGTTCATGT CAATGGTGGG 
GCGTTAGCAT CTGAGCGGGA GACAGACATC CTGGACGATG AATTGCCAAA CCAGGATGGT 
CACAGTGCGG GCAGCATGGG CACACTCTCT TCTCTGGACG GGGTCACCAA CACCAGTGAG 
GGGGGCTACC CAGAGGCCCT GTCCCCACTG ACCAACGGTC TGGACAAGTC CTACCCCATG 
GAGCCTATGG TCAATGGAGG AGGCTACCCC TACGAGTCTG CCAGCCGGGC GGGGCCTGCC 
CATGCTGGCC ACACGGCCCC CATGCGGCCC TCCTACTCTG CACAGGAGGG TTTAGCTGGC 
TACCAGAGGG AGGGGCCCCA CCCAGCCTGG CCACAGCCAG TGACCACCTC CCACTATGCC 
CATGACCCCA GCGGTATGTT CCGCTCTCAA TCCTTTTCGG AAGCTGAACC CCAGCTGCCC 
CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG GAGGCTGTGC AAAGGGGACT GAATTCGTGG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT CGCCCACCTC CACGCCAGCA GGAAAGAGCC 
CACTTGGAGA GTCTTGTAGC CAGCAGGCCC AGCCCTCAGC CATTGGCAGA GACCCCCATC 
CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC TCCCAGCAGG AGATTGAACA GTCCATCGAA 
ACACTCAATA TGCTGATGCT GGACCTGGAG CCAGCCTCCG CTGCTGCCCC ACTACACAAG 
TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT 
GGATCCTCCC GTCAGTCCCA TCCACTGACC CAGTCCAGAT CTGGCTATAT CCCCAGTGGG 
CATTCGTTGG GAACCCCTGA GCCAGCCCCA CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC 
AGGTCTTACT CACCTTATGA CTATCAGCCA TGTTTGGCTG GGCCTAACCA GGATTTCCAT 
TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT GCCTTCCTTC CGACCACCCA CAGCCCTCCA 
GGGCCTCAGC AACCCCCAGC CTCTCTCCCT GGCCTCACTG CTCAGCCTCT GCTCTCACCA 
AAGGAAGCGA CTTCAGACCC CTCCCGGACT CCAGAGGAGG AGCCATTGAA TTTAGAAGGG 
CTGGTGGCCC ACAGGGTAGC AGCGTACAAC GCCAGGCTGC AGGGCATCGG ACACAGTGGC 
AGCTTCCCGC CCCCTCCTCT CCACCGGCTC CAATCTTCCT CCCTCTCTGG GGTACAGGCT 
CGGGAGAAGC AGCCTGCAGA GCCCCCAGCC CCTCTGCGGA GGCGGGCGGC CAGTGATGGA 
CAGTATGAGA ACCAGTCTCC AGAAGCCACA TCCCCTCGTA GCCCTGGGGT TCGCTCCCCT 
GTCCAGTGTG TCTCCCCGGA GCTGGCTCTT ACCATCGCTC TCAATCCTGG AGGGCGGCCC 
AAAGAGCCCC ATTTGCACAG CTACAAGGAG GCCTTCGAGG AGATGGAGGG AACCTCCCCG 
AGCAGCCCAC CACCCAGTGG GGTGCGGTCC CCCCCGGGTC TGGCCAAGAC ACCCCTGTCT 
GCTCTGGGCC TGAAACCTCA CAACCCAGCG GACATCCTGT TGCACCCCAC AGGAGTTACC 
AGAAGACGGA TCCAGCCAGA TTCTCTTTCT CAAGAAATAA TCCCTGTTAA TCTCTTTCTC 
TCTTTTTCCT TTTTTGCTCT TCCTCATCTT CACCACCTGC CCTTCTTTCC TGCCTCTCCT 
TCCTCCCACC TCTCTTCTCG TCTTCCCTCA GAGGAAGATG AGGGGAAGGT GGTGGTCAGG 
CTGTCAGAAG AGCCCCGGAG CTATGTGGAG TCTGTGGCAC GGACAGCGGT GGCTGGACCC 
CGAGCTCAGG ACTCTGAGCC CAAGAGCTTT AGTGCTCCAG CCACCCAGGC CTATGGCCAT 
GAGATACCCC TGAGGAACGG GACCCTGGGT GGCTCCTTTG TCTCCCCCAG CCCCCTCTCC 
ACCAGCAGCC CCATCCTCAG TGCTGACAGC ACTTCAGTGG GGAGTTTCCC GTCGGGAGAG 
AGCAGTGACC AGGGTCCCCG GACGCCCACC CAGCCTCTGT TGGAGTCTGG CTTCCGCTCA 
GGCAGCCTGG GACAGCCCAG CCCGTCTGCC CAGAGAAACT ACCAGAGCTC TTCTCCTCTC 
CCGACTGTGG GCAGTAGCTA CAGCAGCCCC GACTACTCAC TTCAGCATTT CAGCTCCTCT 
CCGGAAAGCC AGGCTCGAGC TCAGTTCAGT GTGGCTGGCG TCCACACGGT GCCTGGGAGC 
CCTCAGGCGC GCCACAGAAC AGTGGGCACC AACACTCCCC CTAGTCCTGG CTTCGGCTGG 
CGGGCCATCA ATCCCAGCAT GGCTGCCCCC AGCAGTCCCA GTTTGAGCCA TCACCAGATG 
ATGGGTCCAC CAGGCACTGG CTTCCATGGT AGCACTGTCT CCAGCCCCCA GAGCAGTGCA 
GCGACCACCC CGGGGAGCCC CAGCCTGTGT CGGCACCCAG CAGGGGTCTA CCAGGTTTCT 
GGCCTCCACA ACAAAGTGGC CACCACCCCG GGGAGTCCCA GCCTGGGCCG GCACCCTGGG 
GCTCACCAAG GCAACCTGGC CTCCGGTCTT CATAGCAATG CAATAGCCAG CCCTGGAAGC 
CCCAGCCTGG GCCGTCACCT CGGAGGGTCT GGATCTGTGG TTCCCGGCAG CCCCTGCTTG 
GACCGGCATG TGGCCTATGG TGGCTATTCT ACCCCGGAGG ATCGGAGACC CACACTGTCC 
CGGCAGAGCA GTGCCTCTGG CTACCAGGCT CCTTCCACGC CCTCCTTCCC TGTCTCCCCT 
GCCTACTACC CTGGCCTGAG CAGCCCTGCC ACCTCCCCGT CACCAGACTC CGCAGCCTTC 
CGGCAAGGGA GCCCAACACC AGCCTTGCCA GAGAAGCGAA GGATGTCAGT GGGAGACCGG 
GCAGGCAGCC TCCCCAACTA TGCCACCATC AATGGGAAGG TGTCTTCGCC TGTCGCCAGC 
GGCATGTCCA GTCCCAGTGG GGGCAGCACC GTCTCCTTCT CCCACACTCT GCCCGACTTC 
TCCAAGTACT CCATGCCAGA CAACAGCCCG GAGACGCGGG CTAAAGTGAA GTTTGTCCAG 
GACACTTCTA AGTATTGGTA CAAGCCTGAG ATCTCCAGGG AGCAGGCCAT CGCGCTCCTC 
AAGGACCAGG AGCCGGGGGC CTTCATCATC CGCGACAGTC ACTCCTTCCG AGGCGCGTAC 
GGGCTGGCCA TGAAGGTGTC TTCGCCACCT CCAACCATCA TGCAGCAGAA TAAAAAAGGA 
GACATGACCC ATGAGCTGGT CAGGCATTTT CTGATAGAGA CTGGCCCCAG AGGAGTCAAG 
CTCAAGGGCT GCCCCAATGA GCCAAACTTC GGATCGCTGT CTGCCCTGGT CTACCAGCAC 
TCCATCATCC CATTGGCCCT GCCTTGCAAG CTGGTCATTC CAAACCGAGA CCCCACAGAT 
GAATCGAAAG ATAGCTCCGG CCCTGCCAAC TCAACTGCAG ACCTGCTGAA ACAAGGGGCA 
GCCTGCAATG TGCTCTTCGT CAACTCTGTG GACATGGAGT CACTCACTGG GCCACAGGCC 
ATCTCTAAAG CCACATCTGA GACGTTGGCT GCAGACCCCA CGCCAGCTGC CACCATCGTT 
CACTTCAAAG TCTCTGCCCA GGGAATCACT CTGACTGACA ACCAGAGAAA GCTCTTTTTC 
AGACGCCACT ACCCTCTCAA CACTGTCACC TTCTGTGACC TGGATCCACA GGAAAGAAAG 
TGGATGAAAA CAGAGGGTGG TGCCCCTGCT AAGCTCTTCG GCTTCGTGGC CCGGAAGCAG 
GGCAGCACCA CGGACAACGC CTGCCACCTC TTTGCTGAGC TTGACCCCAA CCAGCCGGCC 
TCTGCCATCG TCAACTTCGT CTCCAAGGTC ATGCTGAATG CCGGCCAAAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860302.1
CDS	140..4696
Translation	MVGPFAAPYCPPGFSPCFRGQRLSSGRLAVHADTSMGIQGNGYSPGILPLIQSGNSRHRSREVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004813.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004813.2

ORF Insert Sequence:

ATGGTGGGGC CCTTTGCTGC ACCCTACTGC CCTCCTGGGT TCTCCCCCTG CTTCCGTGGG 
CAAAGGCTAT CCTCGGGGCG ACTGGCCGTC CATGCTGATA CCTCTATGGG CATTCAAGGC 
AATGGCTACT CTCCTGGCAT TCTGCCTCTA ATCCAGAGTG GGAACTCCAG ACATCGAAGC 
AGGGAGGTGG TGGGACACAC GCAGGGGCCA CTAGATGGGA GCCTGTATGC TAAGGTGAAG 
AAGAAAGACT CCCTGCACGG CAGCACCGGG GCTGTTAATG CCACACGTCC TACACTGTCG 
GCCACCCCCA ACCACGTGGA ACACACGCTT TCTGTGAGCA GCGACTCGGG CAACTCCACA 
GCCTCCACCA AGACCGACAA GACCGACGAG CCTGTCCCCG GGGCCTCCAG TGCCACTGCT 
GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG GACCGCCTGC TGAGTGGCTT TGGCTTAGAG 
CGAGAGAAGC AAGGCGCCAT GTACCACACC CAGCACCTCA GGTCCCGCCC AGCAGGGGGC 
TCGGCTGTGC CCTCCTCTGG ACGCCACGTT GTCCCAGCCC AGGTTCATGT CAATGGTGGG 
GCGTTAGCAT CTGAGCGGGA GACAGACATC CTGGACGATG AATTGCCAAA CCAGGATGGT 
CACAGTGCGG GCAGCATGGG CACACTCTCT TCTCTGGACG GGGTCACCAA CACCAGTGAG 
GGGGGCTACC CAGAGGCCCT GTCCCCACTG ACCAACGGTC TGGACAAGTC CTACCCCATG 
GAGCCTATGG TCAATGGAGG AGGCTACCCC TACGAGTCTG CCAGCCGGGC GGGGCCTGCC 
CATGCTGGCC ACACGGCCCC CATGCGGCCC TCCTACTCTG CACAGGAGGG TTTAGCTGGC 
TACCAGAGGG AGGGGCCCCA CCCAGCCTGG CCACAGCCAG TGACCACCTC CCACTATGCC 
CATGACCCCA GCGGTATGTT CCGCTCTCAA TCCTTTTCGG AAGCTGAACC CCAGCTGCCC 
CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG GAGGCTGTGC AAAGGGGACT GAATTCGTGG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT CGCCCACCTC CACGCCAGCA GGAAAGAGCC 
CACTTGGAGA GTCTTGTAGC CAGCAGGCCC AGCCCTCAGC CATTGGCAGA GACCCCCATC 
CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC TCCCAGCAGG AGATTGAACA GTCCATCGAA 
ACACTCAATA TGCTGATGCT GGACCTGGAG CCAGCCTCCG CTGCTGCCCC ACTACACAAG 
TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT 
GGATCCTCCC GTCAGTCCCA TCCACTGACC CAGTCCAGAT CTGGCTATAT CCCCAGTGGG 
CATTCGTTGG GAACCCCTGA GCCAGCCCCA CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC 
AGGTCTTACT CACCTTATGA CTATCAGCCA TGTTTGGCTG GGCCTAACCA GGATTTCCAT 
TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT GCCTTCCTTC CGACCACCCA CAGCCCTCCA 
GGGCCTCAGC AACCCCCAGC CTCTCTCCCT GGCCTCACTG CTCAGCCTCT GCTCTCACCA 
AAGGAAGCGA CTTCAGACCC CTCCCGGACT CCAGAGGAGG AGCCATTGAA TTTAGAAGGG 
CTGGTGGCCC ACAGGGTAGC AGCGTACAAC GCCAGGCTGC AGGGCATCGG ACACAGTGGC 
AGCTTCCCGC CCCCTCCTCT CCACCGGCTC CAATCTTCCT CCCTCTCTGG GGTACAGGCT 
CGGGAGAAGC AGCCTGCAGA GCCCCCAGCC CCTCTGCGGA GGCGGGCGGC CAGTGATGGA 
CAGTATGAGA ACCAGTCTCC AGAAGCCACA TCCCCTCGTA GCCCTGGGGT TCGCTCCCCT 
GTCCAGTGTG TCTCCCCGGA GCTGGCTCTT ACCATCGCTC TCAATCCTGG AGGGCGGCCC 
AAAGAGCCCC ATTTGCACAG CTACAAGGAG GCCTTCGAGG AGATGGAGGG AACCTCCCCG 
AGCAGCCCAC CACCCAGTGG GGTGCGGTCC CCCCCGGGTC TGGCCAAGAC ACCCCTGTCT 
GCTCTGGGCC TGAAACCTCA CAACCCAGCG GACATCCTGT TGCACCCCAC AGGAGTTACC 
AGAAGACGGA TCCAGCCAGA TTCTCTTTCT CAAGAAATAA TCCCTGTTAA TCTCTTTCTC 
TCTTTTTCCT TTTTTGCTCT TCCTCATCTT CACCACCTGC CCTTCTTTCC TGCCTCTCCT 
TCCTCCCACC TCTCTTCTCG TCTTCCCTCA GAGGAAGATG AGGGGAAGGT GGTGGTCAGG 
CTGTCAGAAG AGCCCCGGAG CTATGTGGAG TCTGTGGCAC GGACAGCGGT GGCTGGACCC 
CGAGCTCAGG ACTCTGAGCC CAAGAGCTTT AGTGCTCCAG CCACCCAGGC CTATGGCCAT 
GAGATACCCC TGAGGAACGG GACCCTGGGT GGCTCCTTTG TCTCCCCCAG CCCCCTCTCC 
ACCAGCAGCC CCATCCTCAG TGCTGACAGC ACTTCAGTGG GGAGTTTCCC GTCGGGAGAG 
AGCAGTGACC AGGGTCCCCG GACGCCCACC CAGCCTCTGT TGGAGTCTGG CTTCCGCTCA 
GGCAGCCTGG GACAGCCCAG CCCGTCTGCC CAGAGAAACT ACCAGAGCTC TTCTCCTCTC 
CCGACTGTGG GCAGTAGCTA CAGCAGCCCC GACTACTCAC TTCAGCATTT CAGCTCCTCT 
CCGGAAAGCC AGGCTCGAGC TCAGTTCAGT GTGGCTGGCG TCCACACGGT GCCTGGGAGC 
CCTCAGGCGC GCCACAGAAC AGTGGGCACC AACACTCCCC CTAGTCCTGG CTTCGGCTGG 
CGGGCCATCA ATCCCAGCAT GGCTGCCCCC AGCAGTCCCA GTTTGAGCCA TCACCAGATG 
ATGGGTCCAC CAGGCACTGG CTTCCATGGT AGCACTGTCT CCAGCCCCCA GAGCAGTGCA 
GCGACCACCC CGGGGAGCCC CAGCCTGTGT CGGCACCCAG CAGGGGTCTA CCAGGTTTCT 
GGCCTCCACA ACAAAGTGGC CACCACCCCG GGGAGTCCCA GCCTGGGCCG GCACCCTGGG 
GCTCACCAAG GCAACCTGGC CTCCGGTCTT CATAGCAATG CAATAGCCAG CCCTGGAAGC 
CCCAGCCTGG GCCGTCACCT CGGAGGGTCT GGATCTGTGG TTCCCGGCAG CCCCTGCTTG 
GACCGGCATG TGGCCTATGG TGGCTATTCT ACCCCGGAGG ATCGGAGACC CACACTGTCC 
CGGCAGAGCA GTGCCTCTGG CTACCAGGCT CCTTCCACGC CCTCCTTCCC TGTCTCCCCT 
GCCTACTACC CTGGCCTGAG CAGCCCTGCC ACCTCCCCGT CACCAGACTC CGCAGCCTTC 
CGGCAAGGGA GCCCAACACC AGCCTTGCCA GAGAAGCGAA GGATGTCAGT GGGAGACCGG 
GCAGGCAGCC TCCCCAACTA TGCCACCATC AATGGGAAGG TGTCTTCGCC TGTCGCCAGC 
GGCATGTCCA GTCCCAGTGG GGGCAGCACC GTCTCCTTCT CCCACACTCT GCCCGACTTC 
TCCAAGTACT CCATGCCAGA CAACAGCCCG GAGACGCGGG CTAAAGTGAA GTTTGTCCAG 
GACACTTCTA AGTATTGGTA CAAGCCTGAG ATCTCCAGGG AGCAGGCCAT CGCGCTCCTC 
AAGGACCAGG AGCCGGGGGC CTTCATCATC CGCGACAGTC ACTCCTTCCG AGGCGCGTAC 
GGGCTGGCCA TGAAGGTGTC TTCGCCACCT CCAACCATCA TGCAGCAGAA TAAAAAAGGA 
GACATGACCC ATGAGCTGGT CAGGCATTTT CTGATAGAGA CTGGCCCCAG AGGAGTCAAG 
CTCAAGGGCT GCCCCAATGA GCCAAACTTC GGATCGCTGT CTGCCCTGGT CTACCAGCAC 
TCCATCATCC CATTGGCCCT GCCTTGCAAG CTGGTCATTC CAAACCGAGA CCCCACAGAT 
GAATCGAAAG ATAGCTCCGG CCCTGCCAAC TCAACTGCAG ACCTGCTGAA ACAAGGGGCA 
GCCTGCAATG TGCTCTTCGT CAACTCTGTG GACATGGAGT CACTCACTGG GCCACAGGCC 
ATCTCTAAAG CCACATCTGA GACGTTGGCT GCAGACCCCA CGCCAGCTGC CACCATCGTT 
CACTTCAAAG TCTCTGCCCA GGGAATCACT CTGACTGACA ACCAGAGAAA GCTCTTTTTC 
AGACGCCACT ACCCTCTCAA CACTGTCACC TTCTGTGACC TGGATCCACA GGAAAGAAAG 
TGGATGAAAA CAGAGGGTGG TGCCCCTGCT AAGCTCTTCG GCTTCGTGGC CCGGAAGCAG 
GGCAGCACCA CGGACAACGC CTGCCACCTC TTTGCTGAGC TTGACCCCAA CCAGCCGGCC 
TCTGCCATCG TCAACTTCGT CTCCAAGGTC ATGCTGAATG CCGGCCAAAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( NM_001308023.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001294952.1 Homo sapiens tensin 1 (TNS1), transcript variant 3, mRNA.

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CloneID	OHu61088
Clone ID Related Accession (Same CDS sequence)	XM_011511723.2 , NM_001308023.1
Accession Version	NM_001308023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300345.1, BC116188.1, AK309785.1, BC051304.1, BC140942.1, AB209238.1, AC009469.4 and BQ045472.1. On Apr 21, 2015 this sequence version replaced XM_011511720.1. Transcript Variant: This variant (3) differs in the 5' UTR, and lacks two consecutive in-frame exons in the central coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC126910.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGGGGTACAG 
GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCCCCTCTGC GGAGGCGGGC GGCCAGTGAT 
GGACAGTATG AGAACCAGTC TCCAGAAGCC ACATCCCCTC GTAGCCCTGG GGTTCGCTCC 
CCTGTCCAGT GTGTCTCCCC GGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGG 
CCCAAAGAGC CCCATTTGCA CAGCTACAAG GAGGCCTTCG AGGAGATGGA GGGAACCTCC 
CCGAGCAGCC CACCACCCAG TGGGGTGCGG TCCCCCCCGG GTCTGGCCAA GACACCCCTG 
TCTGCTCTGG GCCTGAAACC TCACAACCCA GCGGACATCC TGTTGCACCC CACAGGAGAG 
CCCCGGAGCT ATGTGGAGTC TGTGGCACGG ACAGCGGTGG CTGGACCCCG AGCTCAGGAC 
TCTGAGCCCA AGAGCTTTAG TGCTCCAGCC ACCCAGGCCT ATGGCCATGA GATACCCCTG 
AGGAACGGGA CCCTGGGTGG CTCCTTTGTC TCCCCCAGCC CCCTCTCCAC CAGCAGCCCC 
ATCCTCAGTG CTGACAGCAC TTCAGTGGGG AGTTTCCCGT CGGGAGAGAG CAGTGACCAG 
GGTCCCCGGA CGCCCACCCA GCCTCTGTTG GAGTCTGGCT TCCGCTCAGG CAGCCTGGGA 
CAGCCCAGCC CGTCTGCCCA GAGAAACTAC CAGAGCTCTT CTCCTCTCCC GACTGTGGGC 
AGTAGCTACA GCAGCCCCGA CTACTCACTT CAGCATTTCA GCTCCTCTCC GGAAAGCCAG 
GCTCGAGCTC AGTTCAGTGT GGCTGGCGTC CACACGGTGC CTGGGAGCCC TCAGGCGCGC 
CACAGAACAG TGGGCACCAA CACTCCCCCT AGTCCTGGCT TCGGCTGGCG GGCCATCAAT 
CCCAGCATGG CTGCCCCCAG CAGTCCCAGT TTGAGCCATC ACCAGATGAT GGGTCCACCA 
GGCACTGGCT TCCATGGTAG CACTGTCTCC AGCCCCCAGA GCAGTGCAGC GACCACCCCG 
GGGAGCCCCA GCCTGTGTCG GCACCCAGCA GGGGTCTACC AGGTTTCTGG CCTCCACAAC 
AAAGTGGCCA CCACCCCGGG GAGTCCCAGC CTGGGCCGGC ACCCTGGGGC TCACCAAGGC 
AACCTGGCCT CCGGTCTTCA TAGCAATGCA ATAGCCAGCC CTGGAAGCCC CAGCCTGGGC 
CGTCACCTCG GAGGGTCTGG ATCTGTGGTT CCCGGCAGCC CCTGCTTGGA CCGGCATGTG 
GCCTATGGTG GCTATTCTAC CCCGGAGGAT CGGAGACCCA CACTGTCCCG GCAGAGCAGT 
GCCTCTGGCT ACCAGGCTCC TTCCACGCCC TCCTTCCCTG TCTCCCCTGC CTACTACCCT 
GGCCTGAGCA GCCCTGCCAC CTCCCCGTCA CCAGACTCCG CAGCCTTCCG GCAAGGGAGC 
CCAACACCAG CCTTGCCAGA GAAGCGAAGG ATGTCAGTGG GAGACCGGGC AGGCAGCCTC 
CCCAACTATG CCACCATCAA TGGGAAGGTG TCTTCGCCTG TCGCCAGCGG CATGTCCAGT 
CCCAGTGGGG GCAGCACCGT CTCCTTCTCC CACACTCTGC CCGACTTCTC CAAGTACTCC 
ATGCCAGACA ACAGCCCGGA GACGCGGGCT AAAGTGAAGT TTGTCCAGGA CACTTCTAAG 
TATTGGTACA AGCCTGAGAT CTCCAGGGAG CAGGCCATCG CGCTCCTCAA GGACCAGGAG 
CCGGGGGCCT TCATCATCCG CGACAGTCAC TCCTTCCGAG GCGCGTACGG GCTGGCCATG 
AAGGTGTCTT CGCCACCTCC AACCATCATG CAGCAGAATA AAAAAGGAGA CATGACCCAT 
GAGCTGGTCA GGCATTTTCT GATAGAGACT GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC 
CCCAATGAGC CAAACTTCGG ATCGCTGTCT GCCCTGGTCT ACCAGCACTC CATCATCCCA 
TTGGCCCTGC CTTGCAAGCT GGTCATTCCA AACCGAGACC CCACAGATGA ATCGAAAGAT 
AGCTCCGGCC CTGCCAACTC AACTGCAGAC CTGCTGAAAC AAGGGGCAGC CTGCAATGTG 
CTCTTCGTCA ACTCTGTGGA CATGGAGTCA CTCACTGGGC CACAGGCCAT CTCTAAAGCC 
ACATCTGAGA CGTTGGCTGC AGACCCCACG CCAGCTGCCA CCATCGTTCA CTTCAAAGTC 
TCTGCCCAGG GAATCACTCT GACTGACAAC CAGAGAAAGC TCTTTTTCAG ACGCCACTAC 
CCTCTCAACA CTGTCACCTT CTGTGACCTG GATCCACAGG AAAGAAAGTG GATGAAAACA 
GAGGGTGGTG CCCCTGCTAA GCTCTTCGGC TTCGTGGCCC GGAAGCAGGG CAGCACCACG 
GACAACGCCT GCCACCTCTT TGCTGAGCTT GACCCCAACC AGCCGGCCTC TGCCATCGTC 
AACTTCGTCT CCAAGGTCAT GCTGAATGCC GGCCAAAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294952.1
CDS	190..5334
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	NM_001308023.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308023.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGGGGTACAG 
GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCCCCTCTGC GGAGGCGGGC GGCCAGTGAT 
GGACAGTATG AGAACCAGTC TCCAGAAGCC ACATCCCCTC GTAGCCCTGG GGTTCGCTCC 
CCTGTCCAGT GTGTCTCCCC GGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGG 
CCCAAAGAGC CCCATTTGCA CAGCTACAAG GAGGCCTTCG AGGAGATGGA GGGAACCTCC 
CCGAGCAGCC CACCACCCAG TGGGGTGCGG TCCCCCCCGG GTCTGGCCAA GACACCCCTG 
TCTGCTCTGG GCCTGAAACC TCACAACCCA GCGGACATCC TGTTGCACCC CACAGGAGAG 
CCCCGGAGCT ATGTGGAGTC TGTGGCACGG ACAGCGGTGG CTGGACCCCG AGCTCAGGAC 
TCTGAGCCCA AGAGCTTTAG TGCTCCAGCC ACCCAGGCCT ATGGCCATGA GATACCCCTG 
AGGAACGGGA CCCTGGGTGG CTCCTTTGTC TCCCCCAGCC CCCTCTCCAC CAGCAGCCCC 
ATCCTCAGTG CTGACAGCAC TTCAGTGGGG AGTTTCCCGT CGGGAGAGAG CAGTGACCAG 
GGTCCCCGGA CGCCCACCCA GCCTCTGTTG GAGTCTGGCT TCCGCTCAGG CAGCCTGGGA 
CAGCCCAGCC CGTCTGCCCA GAGAAACTAC CAGAGCTCTT CTCCTCTCCC GACTGTGGGC 
AGTAGCTACA GCAGCCCCGA CTACTCACTT CAGCATTTCA GCTCCTCTCC GGAAAGCCAG 
GCTCGAGCTC AGTTCAGTGT GGCTGGCGTC CACACGGTGC CTGGGAGCCC TCAGGCGCGC 
CACAGAACAG TGGGCACCAA CACTCCCCCT AGTCCTGGCT TCGGCTGGCG GGCCATCAAT 
CCCAGCATGG CTGCCCCCAG CAGTCCCAGT TTGAGCCATC ACCAGATGAT GGGTCCACCA 
GGCACTGGCT TCCATGGTAG CACTGTCTCC AGCCCCCAGA GCAGTGCAGC GACCACCCCG 
GGGAGCCCCA GCCTGTGTCG GCACCCAGCA GGGGTCTACC AGGTTTCTGG CCTCCACAAC 
AAAGTGGCCA CCACCCCGGG GAGTCCCAGC CTGGGCCGGC ACCCTGGGGC TCACCAAGGC 
AACCTGGCCT CCGGTCTTCA TAGCAATGCA ATAGCCAGCC CTGGAAGCCC CAGCCTGGGC 
CGTCACCTCG GAGGGTCTGG ATCTGTGGTT CCCGGCAGCC CCTGCTTGGA CCGGCATGTG 
GCCTATGGTG GCTATTCTAC CCCGGAGGAT CGGAGACCCA CACTGTCCCG GCAGAGCAGT 
GCCTCTGGCT ACCAGGCTCC TTCCACGCCC TCCTTCCCTG TCTCCCCTGC CTACTACCCT 
GGCCTGAGCA GCCCTGCCAC CTCCCCGTCA CCAGACTCCG CAGCCTTCCG GCAAGGGAGC 
CCAACACCAG CCTTGCCAGA GAAGCGAAGG ATGTCAGTGG GAGACCGGGC AGGCAGCCTC 
CCCAACTATG CCACCATCAA TGGGAAGGTG TCTTCGCCTG TCGCCAGCGG CATGTCCAGT 
CCCAGTGGGG GCAGCACCGT CTCCTTCTCC CACACTCTGC CCGACTTCTC CAAGTACTCC 
ATGCCAGACA ACAGCCCGGA GACGCGGGCT AAAGTGAAGT TTGTCCAGGA CACTTCTAAG 
TATTGGTACA AGCCTGAGAT CTCCAGGGAG CAGGCCATCG CGCTCCTCAA GGACCAGGAG 
CCGGGGGCCT TCATCATCCG CGACAGTCAC TCCTTCCGAG GCGCGTACGG GCTGGCCATG 
AAGGTGTCTT CGCCACCTCC AACCATCATG CAGCAGAATA AAAAAGGAGA CATGACCCAT 
GAGCTGGTCA GGCATTTTCT GATAGAGACT GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC 
CCCAATGAGC CAAACTTCGG ATCGCTGTCT GCCCTGGTCT ACCAGCACTC CATCATCCCA 
TTGGCCCTGC CTTGCAAGCT GGTCATTCCA AACCGAGACC CCACAGATGA ATCGAAAGAT 
AGCTCCGGCC CTGCCAACTC AACTGCAGAC CTGCTGAAAC AAGGGGCAGC CTGCAATGTG 
CTCTTCGTCA ACTCTGTGGA CATGGAGTCA CTCACTGGGC CACAGGCCAT CTCTAAAGCC 
ACATCTGAGA CGTTGGCTGC AGACCCCACG CCAGCTGCCA CCATCGTTCA CTTCAAAGTC 
TCTGCCCAGG GAATCACTCT GACTGACAAC CAGAGAAAGC TCTTTTTCAG ACGCCACTAC 
CCTCTCAACA CTGTCACCTT CTGTGACCTG GATCCACAGG AAAGAAAGTG GATGAAAACA 
GAGGGTGGTG CCCCTGCTAA GCTCTTCGGC TTCGTGGCCC GGAAGCAGGG CAGCACCACG 
GACAACGCCT GCCACCTCTT TGCTGAGCTT GACCCCAACC AGCCGGCCTC TGCCATCGTC 
AACTTCGTCT CCAAGGTCAT GCTGAATGCC GGCCAAAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( NM_001308022.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001294951.1 Homo sapiens tensin 1 (TNS1), transcript variant 2, mRNA.

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CloneID	OHu61094
Clone ID Related Accession (Same CDS sequence)	NM_001308022.1
Accession Version	NM_001308022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-01
Organism	Homo sapiens(human)
Product	tensin-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300345.1, BC116188.1, AK309785.1, BC051304.1, AF225896.2, BC116187.1, AB209238.1, AC009469.4 and BQ045472.1. On Apr 21, 2015 this sequence version replaced XM_011511719.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks in-frame exon in the central coding region, and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC116187.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGGGGTACAG 
GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCCCCTCTGC GGAGGCGGGC GGCCAGTGAT 
GGACAGTATG AGAACCAGTC TCCAGAAGCC ACATCCCCTC GTAGCCCTGG GGTTCGCTCC 
CCTGTCCAGT GTGTCTCCCC GGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGG 
CCCAAAGAGC CCCATTTGCA CAGCTACAAG GAGGCCTTCG AGGAGATGGA GGGAACCTCC 
CCGAGCAGCC CACCACCCAG TGGGGTGCGG TCCCCCCCGG GTCTGGCCAA GACACCCCTG 
TCTGCTCTGG GCCTGAAACC TCACAACCCA GCGGACATCC TGTTGCACCC CACAGGAGTT 
ACCAGAAGAC GGATCCAGCC AGAGCCCCGG AGCTATGTGG AGTCTGTGGC ACGGACAGCG 
GTGGCTGGAC CCCGAGCTCA GGACTCTGAG CCCAAGAGCT TTAGTGCTCC AGCCACCCAG 
GCCTATGGCC ATGAGATACC CCTGAGGAAC GGGACCCTGG GTGGCTCCTT TGTCTCCCCC 
AGCCCCCTCT CCACCAGCAG CCCCATCCTC AGTGCTGACA GCACTTCAGT GGGGAGTTTC 
CCGTCGGGAG AGAGCAGTGA CCAGGGTCCC CGGACGCCCA CCCAGCCTCT GTTGGAGTCT 
GGCTTCCGCT CAGGCAGCCT GGGACAGCCC AGCCCGTCTG CCCAGAGAAA CTACCAGAGC 
TCTTCTCCTC TCCCGACTGT GGGCAGTAGC TACAGCAGCC CCGACTACTC ACTTCAGCAT 
TTCAGCTCCT CTCCGGAAAG CCAGGCTCGA GCTCAGTTCA GTGTGGCTGG CGTCCACACG 
GTGCCTGGGA GCCCTCAGGC GCGCCACAGA ACAGTGGGCA CCAACACTCC CCCTAGTCCT 
GGCTTCGGCT GGCGGGCCAT CAATCCCAGC ATGGCTGCCC CCAGCAGTCC CAGTTTGAGC 
CATCACCAGA TGATGGGTCC ACCAGGCACT GGCTTCCATG GTAGCACTGT CTCCAGCCCC 
CAGAGCAGTG CAGCGACCAC CCCGGGGAGC CCCAGCCTGT GTCGGCACCC AGCAGGGGTC 
TACCAGGTTT CTGGCCTCCA CAACAAAGTG GCCACCACCC CGGGGAGTCC CAGCCTGGGC 
CGGCACCCTG GGGCTCACCA AGGCAACCTG GCCTCCGGTC TTCATAGCAA TGCAATAGCC 
AGCCCTGGAA GCCCCAGCCT GGGCCGTCAC CTCGGAGGGT CTGGATCTGT GGTTCCCGGC 
AGCCCCTGCT TGGACCGGCA TGTGGCCTAT GGTGGCTATT CTACCCCGGA GGATCGGAGA 
CCCACACTGT CCCGGCAGAG CAGTGCCTCT GGCTACCAGG CTCCTTCCAC GCCCTCCTTC 
CCTGTCTCCC CTGCCTACTA CCCTGGCCTG AGCAGCCCTG CCACCTCCCC GTCACCAGAC 
TCCGCAGCCT TCCGGCAAGG GAGCCCAACA CCAGCCTTGC CAGAGAAGCG AAGGATGTCA 
GTGGGAGACC GGGCAGGCAG CCTCCCCAAC TATGCCACCA TCAATGGGAA GGTGTCTTCG 
CCTGTCGCCA GCGGCATGTC CAGTCCCAGT GGGGGCAGCA CCGTCTCCTT CTCCCACACT 
CTGCCCGACT TCTCCAAGTA CTCCATGCCA GACAACAGCC CGGAGACGCG GGCTAAAGTG 
AAGTTTGTCC AGGACACTTC TAAGTATTGG TACAAGCCTG AGATCTCCAG GGAGCAGGCC 
ATCGCGCTCC TCAAGGACCA GGAGCCGGGG GCCTTCATCA TCCGCGACAG TCACTCCTTC 
CGAGGCGCGT ACGGGCTGGC CATGAAGGTG TCTTCGCCAC CTCCAACCAT CATGCAGCAG 
AATAAAAAAG ACATGACCCA TGAGCTGGTC AGGCATTTTC TGATAGAGAC TGGCCCCAGA 
GGAGTCAAGC TCAAGGGCTG CCCCAATGAG CCAAACTTCG GATCGCTGTC TGCCCTGGTC 
TACCAGCACT CCATCATCCC ATTGGCCCTG CCTTGCAAGC TGGTCATTCC AAACCGAGAC 
CCCACAGATG AATCGAAAGA TAGCTCCGGC CCTGCCAACT CAACTGCAGA CCTGCTGAAA 
CAAGGGGCAG CCTGCAATGT GCTCTTCGTC AACTCTGTGG ACATGGAGTC ACTCACTGGG 
CCACAGGCCA TCTCTAAAGC CACATCTGAG ACGTTGGCTG CAGACCCCAC GCCAGCTGCC 
ACCATCGTTC ACTTCAAAGT CTCTGCCCAG GGAATCACTC TGACTGACAA CCAGAGAAAG 
CTCTTTTTCA GACGCCACTA CCCTCTCAAC ACTGTCACCT TCTGTGACCT GGATCCACAG 
GAAAGAAAGT GGATGAAAAC AGAGGGTGGT GCCCCTGCTA AGCTCTTCGG CTTCGTGGCC 
CGGAAGCAGG GCAGCACCAC GGACAACGCC TGCCACCTCT TTGCTGAGCT TGACCCCAAC 
CAGCCGGCCT CTGCCATCGT CAACTTCGTC TCCAAGGTCA TGCTGAATGC CGGCCAAAAG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294951.1
CDS	190..5355
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	NM_001308022.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308022.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGGGGTACAG 
GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCCCCTCTGC GGAGGCGGGC GGCCAGTGAT 
GGACAGTATG AGAACCAGTC TCCAGAAGCC ACATCCCCTC GTAGCCCTGG GGTTCGCTCC 
CCTGTCCAGT GTGTCTCCCC GGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGG 
CCCAAAGAGC CCCATTTGCA CAGCTACAAG GAGGCCTTCG AGGAGATGGA GGGAACCTCC 
CCGAGCAGCC CACCACCCAG TGGGGTGCGG TCCCCCCCGG GTCTGGCCAA GACACCCCTG 
TCTGCTCTGG GCCTGAAACC TCACAACCCA GCGGACATCC TGTTGCACCC CACAGGAGTT 
ACCAGAAGAC GGATCCAGCC AGAGCCCCGG AGCTATGTGG AGTCTGTGGC ACGGACAGCG 
GTGGCTGGAC CCCGAGCTCA GGACTCTGAG CCCAAGAGCT TTAGTGCTCC AGCCACCCAG 
GCCTATGGCC ATGAGATACC CCTGAGGAAC GGGACCCTGG GTGGCTCCTT TGTCTCCCCC 
AGCCCCCTCT CCACCAGCAG CCCCATCCTC AGTGCTGACA GCACTTCAGT GGGGAGTTTC 
CCGTCGGGAG AGAGCAGTGA CCAGGGTCCC CGGACGCCCA CCCAGCCTCT GTTGGAGTCT 
GGCTTCCGCT CAGGCAGCCT GGGACAGCCC AGCCCGTCTG CCCAGAGAAA CTACCAGAGC 
TCTTCTCCTC TCCCGACTGT GGGCAGTAGC TACAGCAGCC CCGACTACTC ACTTCAGCAT 
TTCAGCTCCT CTCCGGAAAG CCAGGCTCGA GCTCAGTTCA GTGTGGCTGG CGTCCACACG 
GTGCCTGGGA GCCCTCAGGC GCGCCACAGA ACAGTGGGCA CCAACACTCC CCCTAGTCCT 
GGCTTCGGCT GGCGGGCCAT CAATCCCAGC ATGGCTGCCC CCAGCAGTCC CAGTTTGAGC 
CATCACCAGA TGATGGGTCC ACCAGGCACT GGCTTCCATG GTAGCACTGT CTCCAGCCCC 
CAGAGCAGTG CAGCGACCAC CCCGGGGAGC CCCAGCCTGT GTCGGCACCC AGCAGGGGTC 
TACCAGGTTT CTGGCCTCCA CAACAAAGTG GCCACCACCC CGGGGAGTCC CAGCCTGGGC 
CGGCACCCTG GGGCTCACCA AGGCAACCTG GCCTCCGGTC TTCATAGCAA TGCAATAGCC 
AGCCCTGGAA GCCCCAGCCT GGGCCGTCAC CTCGGAGGGT CTGGATCTGT GGTTCCCGGC 
AGCCCCTGCT TGGACCGGCA TGTGGCCTAT GGTGGCTATT CTACCCCGGA GGATCGGAGA 
CCCACACTGT CCCGGCAGAG CAGTGCCTCT GGCTACCAGG CTCCTTCCAC GCCCTCCTTC 
CCTGTCTCCC CTGCCTACTA CCCTGGCCTG AGCAGCCCTG CCACCTCCCC GTCACCAGAC 
TCCGCAGCCT TCCGGCAAGG GAGCCCAACA CCAGCCTTGC CAGAGAAGCG AAGGATGTCA 
GTGGGAGACC GGGCAGGCAG CCTCCCCAAC TATGCCACCA TCAATGGGAA GGTGTCTTCG 
CCTGTCGCCA GCGGCATGTC CAGTCCCAGT GGGGGCAGCA CCGTCTCCTT CTCCCACACT 
CTGCCCGACT TCTCCAAGTA CTCCATGCCA GACAACAGCC CGGAGACGCG GGCTAAAGTG 
AAGTTTGTCC AGGACACTTC TAAGTATTGG TACAAGCCTG AGATCTCCAG GGAGCAGGCC 
ATCGCGCTCC TCAAGGACCA GGAGCCGGGG GCCTTCATCA TCCGCGACAG TCACTCCTTC 
CGAGGCGCGT ACGGGCTGGC CATGAAGGTG TCTTCGCCAC CTCCAACCAT CATGCAGCAG 
AATAAAAAAG ACATGACCCA TGAGCTGGTC AGGCATTTTC TGATAGAGAC TGGCCCCAGA 
GGAGTCAAGC TCAAGGGCTG CCCCAATGAG CCAAACTTCG GATCGCTGTC TGCCCTGGTC 
TACCAGCACT CCATCATCCC ATTGGCCCTG CCTTGCAAGC TGGTCATTCC AAACCGAGAC 
CCCACAGATG AATCGAAAGA TAGCTCCGGC CCTGCCAACT CAACTGCAGA CCTGCTGAAA 
CAAGGGGCAG CCTGCAATGT GCTCTTCGTC AACTCTGTGG ACATGGAGTC ACTCACTGGG 
CCACAGGCCA TCTCTAAAGC CACATCTGAG ACGTTGGCTG CAGACCCCAC GCCAGCTGCC 
ACCATCGTTC ACTTCAAAGT CTCTGCCCAG GGAATCACTC TGACTGACAA CCAGAGAAAG 
CTCTTTTTCA GACGCCACTA CCCTCTCAAC ACTGTCACCT TCTGTGACCT GGATCCACAG 
GAAAGAAAGT GGATGAAAAC AGAGGGTGGT GCCCCTGCTA AGCTCTTCGG CTTCGTGGCC 
CGGAAGCAGG GCAGCACCAC GGACAACGCC TGCCACCTCT TTGCTGAGCT TGACCCCAAC 
CAGCCGGCCT CTGCCATCGT CAACTTCGTC TCCAAGGTCA TGCTGAATGC CGGCCAAAAG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_011511712.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510014.1 Homo sapiens tensin 1 (TNS1), transcript variant X5, mRNA.

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CloneID	OHu61080
Clone ID Related Accession (Same CDS sequence)	XM_011511712.1
Accession Version	XM_011511712.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGCGTA CAACGCCAGG CTGCAGGGCA TCGGACACAG TGGCAGCTTC 
CCGCCCCCTC CTCTCCACCG GCTCCAATCT TCCTCCCTCT CTGGGGTACA GGCTCGGGAG 
AAGCAGCCTG CAGAGCCCCC AGCCCCTCTG CGGAGGCGGG CGGCCAGTGA TGGACAGTAT 
GAGAACCAGT CTCCAGAAGC CACATCCCCT CGTAGCCCTG GGGTTCGCTC CCCTGTCCAG 
TGTGTCTCCC CGGAGCTGGC TCTTACCATC GCTCTCAATC CTGGAGGGCG GCCCAAAGAG 
CCCCATTTGC ACAGCTACAA GGAGGCCTTC GAGGAGATGG AGGGAACCTC CCCGAGCAGC 
CCACCACCCA GTGGGGTGCG GTCCCCCCCG GGTCTGGCCA AGACACCCCT GTCTGCTCTG 
GGCCTGAAAC CTCACAACCC AGCGGACATC CTGTTGCACC CCACAGGAGA GGAAGATGAG 
GGGAAGGTGG TGGTCAGGCT GTCAGAAGAG CCCCGGAGCT ATGTGGAGTC TGTGGCACGG 
ACAGCGGTGG CTGGACCCCG AGCTCAGGAC TCTGAGCCCA AGAGCTTTAG TGCTCCAGCC 
ACCCAGGCCT ATGGCCATGA GATACCCCTG AGGAACGGGA CCCTGGGTGG CTCCTTTGTC 
TCCCCCAGCC CCCTCTCCAC CAGCAGCCCC ATCCTCAGTG CTGACAGCAC TTCAGTGGGG 
AGTTTCCCGT CGGGAGAGAG CAGTGACCAG GGTCCCCGGA CGCCCACCCA GCCTCTGTTG 
GAGTCTGGCT TCCGCTCAGG CAGCCTGGGA CAGCCCAGCC CGTCTGCCCA GAGAAACTAC 
CAGAGCTCTT CTCCTCTCCC GACTGTGGGC AGTAGCTACA GCAGCCCCGA CTACTCACTT 
CAGCATTTCA GCTCCTCTCC GGAAAGCCAG GCTCGAGCTC AGTTCAGTGT GGCTGGCGTC 
CACACGGTGC CTGGGAGCCC TCAGGCGCGC CACAGAACAG TGGGCACCAA CACTCCCCCT 
AGTCCTGGCT TCGGCTGGCG GGCCATCAAT CCCAGCATGG CTGCCCCCAG CAGTCCCAGT 
TTGAGCCATC ACCAGATGAT GGGTCCACCA GGCACTGGCT TCCATGGTAG CACTGTCTCC 
AGCCCCCAGA GCAGTGCAGC GACCACCCCG GGGAGCCCCA GCCTGTGTCG GCACCCAGCA 
GGGGTCTACC AGGTTTCTGG CCTCCACAAC AAAGTGGCCA CCACCCCGGG GAGTCCCAGC 
CTGGGCCGGC ACCCTGGGGC TCACCAAGGC AACCTGGCCT CCGGTCTTCA TAGCAATGCA 
ATAGCCAGCC CTGGAAGCCC CAGCCTGGGC CGTCACCTCG GAGGGTCTGG ATCTGTGGTT 
CCCGGCAGCC CCTGCTTGGA CCGGCATGTG GCCTATGGTG GCTATTCTAC CCCGGAGGAT 
CGGAGACCCA CACTGTCCCG GCAGAGCAGT GCCTCTGGCT ACCAGGCTCC TTCCACGCCC 
TCCTTCCCTG TCTCCCCTGC CTACTACCCT GGCCTGAGCA GCCCTGCCAC CTCCCCGTCA 
CCAGACTCCG CAGCCTTCCG GCAAGGGAGC CCAACACCAG CCTTGCCAGA GAAGCGAAGG 
ATGTCAGTGG GAGACCGGGC AGGCAGCCTC CCCAACTATG CCACCATCAA TGGGAAGGTG 
TCTTCGCCTG TCGCCAGCGG CATGTCCAGT CCCAGTGGGG GCAGCACCGT CTCCTTCTCC 
CACACTCTGC CCGACTTCTC CAAGTACTCC ATGCCAGACA ACAGCCCGGA GACGCGGGCT 
AAAGTGAAGT TTGTCCAGGA CACTTCTAAG TATTGGTACA AGCCTGAGAT CTCCAGGGAG 
CAGGCCATCG CGCTCCTCAA GGACCAGGAG CCGGGGGCCT TCATCATCCG CGACAGTCAC 
TCCTTCCGAG GCGCGTACGG GCTGGCCATG AAGGTGTCTT CGCCACCTCC AACCATCATG 
CAGCAGAATA AAAAAGGAGA CATGACCCAT GAGCTGGTCA GGCATTTTCT GATAGAGACT 
GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC CCCAATGAGC CAAACTTCGG ATCGCTGTCT 
GCCCTGGTCT ACCAGCACTC CATCATCCCA TTGGCCCTGC CTTGCAAGCT GGTCATTCCA 
AACCGAGACC CCACAGATGA ATCGAAAGAT AGCTCCGGCC CTGCCAACTC AACTGCAGAC 
CTGCTGAAAC AAGGGGCAGC CTGCAATGTG CTCTTCGTCA ACTCTGTGGA CATGGAGTCA 
CTCACTGGGC CACAGGCCAT CTCTAAAGCC ACATCTGAGA CGTTGGCTGC AGACCCCACG 
CCAGCTGCCA CCATCGTTCA CTTCAAAGTC TCTGCCCAGG GAATCACTCT GACTGACAAC 
CAGAGAAAGC TCTTTTTCAG ACGCCACTAC CCTCTCAACA CTGTCACCTT CTGTGACCTG 
GATCCACAGG AAAGAAAGTG GATGAAAACA GAGGGTGGTG CCCCTGCTAA GCTCTTCGGC 
TTCGTGGCCC GGAAGCAGGG CAGCACCACG GACAACGCCT GCCACCTCTT TGCTGAGCTT 
GACCCCAACC AGCCGGCCTC TGCCATCGTC AACTTCGTCT CCAAGGTCAT GCTGAATGCC 
GGCCAAAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510014.1
CDS	55..5589
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCINGNKQNTNSQSIGSISEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_011511712.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511712.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGCGTA CAACGCCAGG CTGCAGGGCA TCGGACACAG TGGCAGCTTC 
CCGCCCCCTC CTCTCCACCG GCTCCAATCT TCCTCCCTCT CTGGGGTACA GGCTCGGGAG 
AAGCAGCCTG CAGAGCCCCC AGCCCCTCTG CGGAGGCGGG CGGCCAGTGA TGGACAGTAT 
GAGAACCAGT CTCCAGAAGC CACATCCCCT CGTAGCCCTG GGGTTCGCTC CCCTGTCCAG 
TGTGTCTCCC CGGAGCTGGC TCTTACCATC GCTCTCAATC CTGGAGGGCG GCCCAAAGAG 
CCCCATTTGC ACAGCTACAA GGAGGCCTTC GAGGAGATGG AGGGAACCTC CCCGAGCAGC 
CCACCACCCA GTGGGGTGCG GTCCCCCCCG GGTCTGGCCA AGACACCCCT GTCTGCTCTG 
GGCCTGAAAC CTCACAACCC AGCGGACATC CTGTTGCACC CCACAGGAGA GGAAGATGAG 
GGGAAGGTGG TGGTCAGGCT GTCAGAAGAG CCCCGGAGCT ATGTGGAGTC TGTGGCACGG 
ACAGCGGTGG CTGGACCCCG AGCTCAGGAC TCTGAGCCCA AGAGCTTTAG TGCTCCAGCC 
ACCCAGGCCT ATGGCCATGA GATACCCCTG AGGAACGGGA CCCTGGGTGG CTCCTTTGTC 
TCCCCCAGCC CCCTCTCCAC CAGCAGCCCC ATCCTCAGTG CTGACAGCAC TTCAGTGGGG 
AGTTTCCCGT CGGGAGAGAG CAGTGACCAG GGTCCCCGGA CGCCCACCCA GCCTCTGTTG 
GAGTCTGGCT TCCGCTCAGG CAGCCTGGGA CAGCCCAGCC CGTCTGCCCA GAGAAACTAC 
CAGAGCTCTT CTCCTCTCCC GACTGTGGGC AGTAGCTACA GCAGCCCCGA CTACTCACTT 
CAGCATTTCA GCTCCTCTCC GGAAAGCCAG GCTCGAGCTC AGTTCAGTGT GGCTGGCGTC 
CACACGGTGC CTGGGAGCCC TCAGGCGCGC CACAGAACAG TGGGCACCAA CACTCCCCCT 
AGTCCTGGCT TCGGCTGGCG GGCCATCAAT CCCAGCATGG CTGCCCCCAG CAGTCCCAGT 
TTGAGCCATC ACCAGATGAT GGGTCCACCA GGCACTGGCT TCCATGGTAG CACTGTCTCC 
AGCCCCCAGA GCAGTGCAGC GACCACCCCG GGGAGCCCCA GCCTGTGTCG GCACCCAGCA 
GGGGTCTACC AGGTTTCTGG CCTCCACAAC AAAGTGGCCA CCACCCCGGG GAGTCCCAGC 
CTGGGCCGGC ACCCTGGGGC TCACCAAGGC AACCTGGCCT CCGGTCTTCA TAGCAATGCA 
ATAGCCAGCC CTGGAAGCCC CAGCCTGGGC CGTCACCTCG GAGGGTCTGG ATCTGTGGTT 
CCCGGCAGCC CCTGCTTGGA CCGGCATGTG GCCTATGGTG GCTATTCTAC CCCGGAGGAT 
CGGAGACCCA CACTGTCCCG GCAGAGCAGT GCCTCTGGCT ACCAGGCTCC TTCCACGCCC 
TCCTTCCCTG TCTCCCCTGC CTACTACCCT GGCCTGAGCA GCCCTGCCAC CTCCCCGTCA 
CCAGACTCCG CAGCCTTCCG GCAAGGGAGC CCAACACCAG CCTTGCCAGA GAAGCGAAGG 
ATGTCAGTGG GAGACCGGGC AGGCAGCCTC CCCAACTATG CCACCATCAA TGGGAAGGTG 
TCTTCGCCTG TCGCCAGCGG CATGTCCAGT CCCAGTGGGG GCAGCACCGT CTCCTTCTCC 
CACACTCTGC CCGACTTCTC CAAGTACTCC ATGCCAGACA ACAGCCCGGA GACGCGGGCT 
AAAGTGAAGT TTGTCCAGGA CACTTCTAAG TATTGGTACA AGCCTGAGAT CTCCAGGGAG 
CAGGCCATCG CGCTCCTCAA GGACCAGGAG CCGGGGGCCT TCATCATCCG CGACAGTCAC 
TCCTTCCGAG GCGCGTACGG GCTGGCCATG AAGGTGTCTT CGCCACCTCC AACCATCATG 
CAGCAGAATA AAAAAGGAGA CATGACCCAT GAGCTGGTCA GGCATTTTCT GATAGAGACT 
GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC CCCAATGAGC CAAACTTCGG ATCGCTGTCT 
GCCCTGGTCT ACCAGCACTC CATCATCCCA TTGGCCCTGC CTTGCAAGCT GGTCATTCCA 
AACCGAGACC CCACAGATGA ATCGAAAGAT AGCTCCGGCC CTGCCAACTC AACTGCAGAC 
CTGCTGAAAC AAGGGGCAGC CTGCAATGTG CTCTTCGTCA ACTCTGTGGA CATGGAGTCA 
CTCACTGGGC CACAGGCCAT CTCTAAAGCC ACATCTGAGA CGTTGGCTGC AGACCCCACG 
CCAGCTGCCA CCATCGTTCA CTTCAAAGTC TCTGCCCAGG GAATCACTCT GACTGACAAC 
CAGAGAAAGC TCTTTTTCAG ACGCCACTAC CCTCTCAACA CTGTCACCTT CTGTGACCTG 
GATCCACAGG AAAGAAAGTG GATGAAAACA GAGGGTGGTG CCCCTGCTAA GCTCTTCGGC 
TTCGTGGCCC GGAAGCAGGG CAGCACCACG GACAACGCCT GCCACCTCTT TGCTGAGCTT 
GACCCCAACC AGCCGGCCTC TGCCATCGTC AACTTCGTCT CCAAGGTCAT GCTGAATGCC 
GGCCAAAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_011511713.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510015.1 Homo sapiens tensin 1 (TNS1), transcript variant X6, mRNA.

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CloneID	OHu61081
Clone ID Related Accession (Same CDS sequence)	XM_011511713.1
Accession Version	XM_011511713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGCGTA CAACGCCAGG CTGCAGGGCA TCGGACACAG TGGCAGCTTC 
CCGCCCCCTC CTCTCCACCG GCTCCAATCT TCCTCCCTCT CTGGGGTACA GGCTCGGGAG 
AAGCAGCCTG CAGAGCCCCC AGCCCCTCTG CGGAGGCGGG CGGCCAGTGA TGGACAGTAT 
GAGAACCAGT CTCCAGAAGC CACATCCCCT CGTAGCCCTG GGGTTCGCTC CCCTGTCCAG 
TGTGTCTCCC CGGAGCTGGC TCTTACCATC GCTCTCAATC CTGGAGGGCG GCCCAAAGAG 
CCCCATTTGC ACAGCTACAA GGAGGCCTTC GAGGAGATGG AGGGAACCTC CCCGAGCAGC 
CCACCACCCA GTGGGGTGCG GTCCCCCCCG GGTCTGGCCA AGACACCCCT GTCTGCTCTG 
GGCCTGAAAC CTCACAACCC AGCGGACATC CTGTTGCACC CCACAGGAGT TACCAGAAGA 
CGGATCCAGC CAGAGCCCCG GAGCTATGTG GAGTCTGTGG CACGGACAGC GGTGGCTGGA 
CCCCGAGCTC AGGACTCTGA GCCCAAGAGC TTTAGTGCTC CAGCCACCCA GGCCTATGGC 
CATGAGATAC CCCTGAGGAA CGGGACCCTG GGTGGCTCCT TTGTCTCCCC CAGCCCCCTC 
TCCACCAGCA GCCCCATCCT CAGTGCTGAC AGCACTTCAG TGGGGAGTTT CCCGTCGGGA 
GAGAGCAGTG ACCAGGGTCC CCGGACGCCC ACCCAGCCTC TGTTGGAGTC TGGCTTCCGC 
TCAGGCAGCC TGGGACAGCC CAGCCCGTCT GCCCAGAGAA ACTACCAGAG CTCTTCTCCT 
CTCCCGACTG TGGGCAGTAG CTACAGCAGC CCCGACTACT CACTTCAGCA TTTCAGCTCC 
TCTCCGGAAA GCCAGGCTCG AGCTCAGTTC AGTGTGGCTG GCGTCCACAC GGTGCCTGGG 
AGCCCTCAGG CGCGCCACAG AACAGTGGGC ACCAACACTC CCCCTAGTCC TGGCTTCGGC 
TGGCGGGCCA TCAATCCCAG CATGGCTGCC CCCAGCAGTC CCAGTTTGAG CCATCACCAG 
ATGATGGGTC CACCAGGCAC TGGCTTCCAT GGTAGCACTG TCTCCAGCCC CCAGAGCAGT 
GCAGCGACCA CCCCGGGGAG CCCCAGCCTG TGTCGGCACC CAGCAGGGGT CTACCAGGTT 
TCTGGCCTCC ACAACAAAGT GGCCACCACC CCGGGGAGTC CCAGCCTGGG CCGGCACCCT 
GGGGCTCACC AAGGCAACCT GGCCTCCGGT CTTCATAGCA ATGCAATAGC CAGCCCTGGA 
AGCCCCAGCC TGGGCCGTCA CCTCGGAGGG TCTGGATCTG TGGTTCCCGG CAGCCCCTGC 
TTGGACCGGC ATGTGGCCTA TGGTGGCTAT TCTACCCCGG AGGATCGGAG ACCCACACTG 
TCCCGGCAGA GCAGTGCCTC TGGCTACCAG GCTCCTTCCA CGCCCTCCTT CCCTGTCTCC 
CCTGCCTACT ACCCTGGCCT GAGCAGCCCT GCCACCTCCC CGTCACCAGA CTCCGCAGCC 
TTCCGGCAAG GGAGCCCAAC ACCAGCCTTG CCAGAGAAGC GAAGGATGTC AGTGGGAGAC 
CGGGCAGGCA GCCTCCCCAA CTATGCCACC ATCAATGGGA AGGTGTCTTC GCCTGTCGCC 
AGCGGCATGT CCAGTCCCAG TGGGGGCAGC ACCGTCTCCT TCTCCCACAC TCTGCCCGAC 
TTCTCCAAGT ACTCCATGCC AGACAACAGC CCGGAGACGC GGGCTAAAGT GAAGTTTGTC 
CAGGACACTT CTAAGTATTG GTACAAGCCT GAGATCTCCA GGGAGCAGGC CATCGCGCTC 
CTCAAGGACC AGGAGCCGGG GGCCTTCATC ATCCGCGACA GTCACTCCTT CCGAGGCGCG 
TACGGGCTGG CCATGAAGGT GTCTTCGCCA CCTCCAACCA TCATGCAGCA GAATAAAAAA 
GGAGACATGA CCCATGAGCT GGTCAGGCAT TTTCTGATAG AGACTGGCCC CAGAGGAGTC 
AAGCTCAAGG GCTGCCCCAA TGAGCCAAAC TTCGGATCGC TGTCTGCCCT GGTCTACCAG 
CACTCCATCA TCCCATTGGC CCTGCCTTGC AAGCTGGTCA TTCCAAACCG AGACCCCACA 
GATGAATCGA AAGATAGCTC CGGCCCTGCC AACTCAACTG CAGACCTGCT GAAACAAGGG 
GCAGCCTGCA ATGTGCTCTT CGTCAACTCT GTGGACATGG AGTCACTCAC TGGGCCACAG 
GCCATCTCTA AAGCCACATC TGAGACGTTG GCTGCAGACC CCACGCCAGC TGCCACCATC 
GTTCACTTCA AAGTCTCTGC CCAGGGAATC ACTCTGACTG ACAACCAGAG AAAGCTCTTT 
TTCAGACGCC ACTACCCTCT CAACACTGTC ACCTTCTGTG ACCTGGATCC ACAGGAAAGA 
AAGTGGATGA AAACAGAGGG TGGTGCCCCT GCTAAGCTCT TCGGCTTCGT GGCCCGGAAG 
CAGGGCAGCA CCACGGACAA CGCCTGCCAC CTCTTTGCTG AGCTTGACCC CAACCAGCCG 
GCCTCTGCCA TCGTCAACTT CGTCTCCAAG GTCATGCTGA ATGCCGGCCA AAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510015.1
CDS	55..5574
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCINGNKQNTNSQSIGSISEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_011511713.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511713.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGCGTA CAACGCCAGG CTGCAGGGCA TCGGACACAG TGGCAGCTTC 
CCGCCCCCTC CTCTCCACCG GCTCCAATCT TCCTCCCTCT CTGGGGTACA GGCTCGGGAG 
AAGCAGCCTG CAGAGCCCCC AGCCCCTCTG CGGAGGCGGG CGGCCAGTGA TGGACAGTAT 
GAGAACCAGT CTCCAGAAGC CACATCCCCT CGTAGCCCTG GGGTTCGCTC CCCTGTCCAG 
TGTGTCTCCC CGGAGCTGGC TCTTACCATC GCTCTCAATC CTGGAGGGCG GCCCAAAGAG 
CCCCATTTGC ACAGCTACAA GGAGGCCTTC GAGGAGATGG AGGGAACCTC CCCGAGCAGC 
CCACCACCCA GTGGGGTGCG GTCCCCCCCG GGTCTGGCCA AGACACCCCT GTCTGCTCTG 
GGCCTGAAAC CTCACAACCC AGCGGACATC CTGTTGCACC CCACAGGAGT TACCAGAAGA 
CGGATCCAGC CAGAGCCCCG GAGCTATGTG GAGTCTGTGG CACGGACAGC GGTGGCTGGA 
CCCCGAGCTC AGGACTCTGA GCCCAAGAGC TTTAGTGCTC CAGCCACCCA GGCCTATGGC 
CATGAGATAC CCCTGAGGAA CGGGACCCTG GGTGGCTCCT TTGTCTCCCC CAGCCCCCTC 
TCCACCAGCA GCCCCATCCT CAGTGCTGAC AGCACTTCAG TGGGGAGTTT CCCGTCGGGA 
GAGAGCAGTG ACCAGGGTCC CCGGACGCCC ACCCAGCCTC TGTTGGAGTC TGGCTTCCGC 
TCAGGCAGCC TGGGACAGCC CAGCCCGTCT GCCCAGAGAA ACTACCAGAG CTCTTCTCCT 
CTCCCGACTG TGGGCAGTAG CTACAGCAGC CCCGACTACT CACTTCAGCA TTTCAGCTCC 
TCTCCGGAAA GCCAGGCTCG AGCTCAGTTC AGTGTGGCTG GCGTCCACAC GGTGCCTGGG 
AGCCCTCAGG CGCGCCACAG AACAGTGGGC ACCAACACTC CCCCTAGTCC TGGCTTCGGC 
TGGCGGGCCA TCAATCCCAG CATGGCTGCC CCCAGCAGTC CCAGTTTGAG CCATCACCAG 
ATGATGGGTC CACCAGGCAC TGGCTTCCAT GGTAGCACTG TCTCCAGCCC CCAGAGCAGT 
GCAGCGACCA CCCCGGGGAG CCCCAGCCTG TGTCGGCACC CAGCAGGGGT CTACCAGGTT 
TCTGGCCTCC ACAACAAAGT GGCCACCACC CCGGGGAGTC CCAGCCTGGG CCGGCACCCT 
GGGGCTCACC AAGGCAACCT GGCCTCCGGT CTTCATAGCA ATGCAATAGC CAGCCCTGGA 
AGCCCCAGCC TGGGCCGTCA CCTCGGAGGG TCTGGATCTG TGGTTCCCGG CAGCCCCTGC 
TTGGACCGGC ATGTGGCCTA TGGTGGCTAT TCTACCCCGG AGGATCGGAG ACCCACACTG 
TCCCGGCAGA GCAGTGCCTC TGGCTACCAG GCTCCTTCCA CGCCCTCCTT CCCTGTCTCC 
CCTGCCTACT ACCCTGGCCT GAGCAGCCCT GCCACCTCCC CGTCACCAGA CTCCGCAGCC 
TTCCGGCAAG GGAGCCCAAC ACCAGCCTTG CCAGAGAAGC GAAGGATGTC AGTGGGAGAC 
CGGGCAGGCA GCCTCCCCAA CTATGCCACC ATCAATGGGA AGGTGTCTTC GCCTGTCGCC 
AGCGGCATGT CCAGTCCCAG TGGGGGCAGC ACCGTCTCCT TCTCCCACAC TCTGCCCGAC 
TTCTCCAAGT ACTCCATGCC AGACAACAGC CCGGAGACGC GGGCTAAAGT GAAGTTTGTC 
CAGGACACTT CTAAGTATTG GTACAAGCCT GAGATCTCCA GGGAGCAGGC CATCGCGCTC 
CTCAAGGACC AGGAGCCGGG GGCCTTCATC ATCCGCGACA GTCACTCCTT CCGAGGCGCG 
TACGGGCTGG CCATGAAGGT GTCTTCGCCA CCTCCAACCA TCATGCAGCA GAATAAAAAA 
GGAGACATGA CCCATGAGCT GGTCAGGCAT TTTCTGATAG AGACTGGCCC CAGAGGAGTC 
AAGCTCAAGG GCTGCCCCAA TGAGCCAAAC TTCGGATCGC TGTCTGCCCT GGTCTACCAG 
CACTCCATCA TCCCATTGGC CCTGCCTTGC AAGCTGGTCA TTCCAAACCG AGACCCCACA 
GATGAATCGA AAGATAGCTC CGGCCCTGCC AACTCAACTG CAGACCTGCT GAAACAAGGG 
GCAGCCTGCA ATGTGCTCTT CGTCAACTCT GTGGACATGG AGTCACTCAC TGGGCCACAG 
GCCATCTCTA AAGCCACATC TGAGACGTTG GCTGCAGACC CCACGCCAGC TGCCACCATC 
GTTCACTTCA AAGTCTCTGC CCAGGGAATC ACTCTGACTG ACAACCAGAG AAAGCTCTTT 
TTCAGACGCC ACTACCCTCT CAACACTGTC ACCTTCTGTG ACCTGGATCC ACAGGAAAGA 
AAGTGGATGA AAACAGAGGG TGGTGCCCCT GCTAAGCTCT TCGGCTTCGT GGCCCGGAAG 
CAGGGCAGCA CCACGGACAA CGCCTGCCAC CTCTTTGCTG AGCTTGACCC CAACCAGCCG 
GCCTCTGCCA TCGTCAACTT CGTCTCCAAG GTCATGCTGA ATGCCGGCCA AAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_011511715.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510017.1 Homo sapiens tensin 1 (TNS1), transcript variant X7, mRNA.

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CloneID	OHu61083
Clone ID Related Accession (Same CDS sequence)	XM_011511715.1
Accession Version	XM_011511715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGCGTA CAACGCCAGG CTGCAGGGCA TCGGACACAG TGGCAGCTTC 
CCGCCCCCTC CTCTCCACCG GCTCCAATCT TCCTCCCTCT CTGGGGTACA GGCTCGGGAG 
AAGCAGCCTG CAGAGCCCCC AGCCCCTCTG CGGAGGCGGG CGGCCAGTGA TGGACAGTAT 
GAGAACCAGT CTCCAGAAGC CACATCCCCT CGTAGCCCTG GGGTTCGCTC CCCTGTCCAG 
TGTGTCTCCC CGGAGCTGGC TCTTACCATC GCTCTCAATC CTGGAGGGCG GCCCAAAGAG 
CCCCATTTGC ACAGCTACAA GGAGGCCTTC GAGGAGATGG AGGGAACCTC CCCGAGCAGC 
CCACCACCCA GTGGGGTGCG GTCCCCCCCG GGTCTGGCCA AGACACCCCT GTCTGCTCTG 
GGCCTGAAAC CTCACAACCC AGCGGACATC CTGTTGCACC CCACAGGAGA GCCCCGGAGC 
TATGTGGAGT CTGTGGCACG GACAGCGGTG GCTGGACCCC GAGCTCAGGA CTCTGAGCCC 
AAGAGCTTTA GTGCTCCAGC CACCCAGGCC TATGGCCATG AGATACCCCT GAGGAACGGG 
ACCCTGGGTG GCTCCTTTGT CTCCCCCAGC CCCCTCTCCA CCAGCAGCCC CATCCTCAGT 
GCTGACAGCA CTTCAGTGGG GAGTTTCCCG TCGGGAGAGA GCAGTGACCA GGGTCCCCGG 
ACGCCCACCC AGCCTCTGTT GGAGTCTGGC TTCCGCTCAG GCAGCCTGGG ACAGCCCAGC 
CCGTCTGCCC AGAGAAACTA CCAGAGCTCT TCTCCTCTCC CGACTGTGGG CAGTAGCTAC 
AGCAGCCCCG ACTACTCACT TCAGCATTTC AGCTCCTCTC CGGAAAGCCA GGCTCGAGCT 
CAGTTCAGTG TGGCTGGCGT CCACACGGTG CCTGGGAGCC CTCAGGCGCG CCACAGAACA 
GTGGGCACCA ACACTCCCCC TAGTCCTGGC TTCGGCTGGC GGGCCATCAA TCCCAGCATG 
GCTGCCCCCA GCAGTCCCAG TTTGAGCCAT CACCAGATGA TGGGTCCACC AGGCACTGGC 
TTCCATGGTA GCACTGTCTC CAGCCCCCAG AGCAGTGCAG CGACCACCCC GGGGAGCCCC 
AGCCTGTGTC GGCACCCAGC AGGGGTCTAC CAGGTTTCTG GCCTCCACAA CAAAGTGGCC 
ACCACCCCGG GGAGTCCCAG CCTGGGCCGG CACCCTGGGG CTCACCAAGG CAACCTGGCC 
TCCGGTCTTC ATAGCAATGC AATAGCCAGC CCTGGAAGCC CCAGCCTGGG CCGTCACCTC 
GGAGGGTCTG GATCTGTGGT TCCCGGCAGC CCCTGCTTGG ACCGGCATGT GGCCTATGGT 
GGCTATTCTA CCCCGGAGGA TCGGAGACCC ACACTGTCCC GGCAGAGCAG TGCCTCTGGC 
TACCAGGCTC CTTCCACGCC CTCCTTCCCT GTCTCCCCTG CCTACTACCC TGGCCTGAGC 
AGCCCTGCCA CCTCCCCGTC ACCAGACTCC GCAGCCTTCC GGCAAGGGAG CCCAACACCA 
GCCTTGCCAG AGAAGCGAAG GATGTCAGTG GGAGACCGGG CAGGCAGCCT CCCCAACTAT 
GCCACCATCA ATGGGAAGGT GTCTTCGCCT GTCGCCAGCG GCATGTCCAG TCCCAGTGGG 
GGCAGCACCG TCTCCTTCTC CCACACTCTG CCCGACTTCT CCAAGTACTC CATGCCAGAC 
AACAGCCCGG AGACGCGGGC TAAAGTGAAG TTTGTCCAGG ACACTTCTAA GTATTGGTAC 
AAGCCTGAGA TCTCCAGGGA GCAGGCCATC GCGCTCCTCA AGGACCAGGA GCCGGGGGCC 
TTCATCATCC GCGACAGTCA CTCCTTCCGA GGCGCGTACG GGCTGGCCAT GAAGGTGTCT 
TCGCCACCTC CAACCATCAT GCAGCAGAAT AAAAAAGGAG ACATGACCCA TGAGCTGGTC 
AGGCATTTTC TGATAGAGAC TGGCCCCAGA GGAGTCAAGC TCAAGGGCTG CCCCAATGAG 
CCAAACTTCG GATCGCTGTC TGCCCTGGTC TACCAGCACT CCATCATCCC ATTGGCCCTG 
CCTTGCAAGC TGGTCATTCC AAACCGAGAC CCCACAGATG AATCGAAAGA TAGCTCCGGC 
CCTGCCAACT CAACTGCAGA CCTGCTGAAA CAAGGGGCAG CCTGCAATGT GCTCTTCGTC 
AACTCTGTGG ACATGGAGTC ACTCACTGGG CCACAGGCCA TCTCTAAAGC CACATCTGAG 
ACGTTGGCTG CAGACCCCAC GCCAGCTGCC ACCATCGTTC ACTTCAAAGT CTCTGCCCAG 
GGAATCACTC TGACTGACAA CCAGAGAAAG CTCTTTTTCA GACGCCACTA CCCTCTCAAC 
ACTGTCACCT TCTGTGACCT GGATCCACAG GAAAGAAAGT GGATGAAAAC AGAGGGTGGT 
GCCCCTGCTA AGCTCTTCGG CTTCGTGGCC CGGAAGCAGG GCAGCACCAC GGACAACGCC 
TGCCACCTCT TTGCTGAGCT TGACCCCAAC CAGCCGGCCT CTGCCATCGT CAACTTCGTC 
TCCAAGGTCA TGCTGAATGC CGGCCAAAAG AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510017.1
CDS	55..5550
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCINGNKQNTNSQSIGSISEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_011511715.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511715.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGCGTA CAACGCCAGG CTGCAGGGCA TCGGACACAG TGGCAGCTTC 
CCGCCCCCTC CTCTCCACCG GCTCCAATCT TCCTCCCTCT CTGGGGTACA GGCTCGGGAG 
AAGCAGCCTG CAGAGCCCCC AGCCCCTCTG CGGAGGCGGG CGGCCAGTGA TGGACAGTAT 
GAGAACCAGT CTCCAGAAGC CACATCCCCT CGTAGCCCTG GGGTTCGCTC CCCTGTCCAG 
TGTGTCTCCC CGGAGCTGGC TCTTACCATC GCTCTCAATC CTGGAGGGCG GCCCAAAGAG 
CCCCATTTGC ACAGCTACAA GGAGGCCTTC GAGGAGATGG AGGGAACCTC CCCGAGCAGC 
CCACCACCCA GTGGGGTGCG GTCCCCCCCG GGTCTGGCCA AGACACCCCT GTCTGCTCTG 
GGCCTGAAAC CTCACAACCC AGCGGACATC CTGTTGCACC CCACAGGAGA GCCCCGGAGC 
TATGTGGAGT CTGTGGCACG GACAGCGGTG GCTGGACCCC GAGCTCAGGA CTCTGAGCCC 
AAGAGCTTTA GTGCTCCAGC CACCCAGGCC TATGGCCATG AGATACCCCT GAGGAACGGG 
ACCCTGGGTG GCTCCTTTGT CTCCCCCAGC CCCCTCTCCA CCAGCAGCCC CATCCTCAGT 
GCTGACAGCA CTTCAGTGGG GAGTTTCCCG TCGGGAGAGA GCAGTGACCA GGGTCCCCGG 
ACGCCCACCC AGCCTCTGTT GGAGTCTGGC TTCCGCTCAG GCAGCCTGGG ACAGCCCAGC 
CCGTCTGCCC AGAGAAACTA CCAGAGCTCT TCTCCTCTCC CGACTGTGGG CAGTAGCTAC 
AGCAGCCCCG ACTACTCACT TCAGCATTTC AGCTCCTCTC CGGAAAGCCA GGCTCGAGCT 
CAGTTCAGTG TGGCTGGCGT CCACACGGTG CCTGGGAGCC CTCAGGCGCG CCACAGAACA 
GTGGGCACCA ACACTCCCCC TAGTCCTGGC TTCGGCTGGC GGGCCATCAA TCCCAGCATG 
GCTGCCCCCA GCAGTCCCAG TTTGAGCCAT CACCAGATGA TGGGTCCACC AGGCACTGGC 
TTCCATGGTA GCACTGTCTC CAGCCCCCAG AGCAGTGCAG CGACCACCCC GGGGAGCCCC 
AGCCTGTGTC GGCACCCAGC AGGGGTCTAC CAGGTTTCTG GCCTCCACAA CAAAGTGGCC 
ACCACCCCGG GGAGTCCCAG CCTGGGCCGG CACCCTGGGG CTCACCAAGG CAACCTGGCC 
TCCGGTCTTC ATAGCAATGC AATAGCCAGC CCTGGAAGCC CCAGCCTGGG CCGTCACCTC 
GGAGGGTCTG GATCTGTGGT TCCCGGCAGC CCCTGCTTGG ACCGGCATGT GGCCTATGGT 
GGCTATTCTA CCCCGGAGGA TCGGAGACCC ACACTGTCCC GGCAGAGCAG TGCCTCTGGC 
TACCAGGCTC CTTCCACGCC CTCCTTCCCT GTCTCCCCTG CCTACTACCC TGGCCTGAGC 
AGCCCTGCCA CCTCCCCGTC ACCAGACTCC GCAGCCTTCC GGCAAGGGAG CCCAACACCA 
GCCTTGCCAG AGAAGCGAAG GATGTCAGTG GGAGACCGGG CAGGCAGCCT CCCCAACTAT 
GCCACCATCA ATGGGAAGGT GTCTTCGCCT GTCGCCAGCG GCATGTCCAG TCCCAGTGGG 
GGCAGCACCG TCTCCTTCTC CCACACTCTG CCCGACTTCT CCAAGTACTC CATGCCAGAC 
AACAGCCCGG AGACGCGGGC TAAAGTGAAG TTTGTCCAGG ACACTTCTAA GTATTGGTAC 
AAGCCTGAGA TCTCCAGGGA GCAGGCCATC GCGCTCCTCA AGGACCAGGA GCCGGGGGCC 
TTCATCATCC GCGACAGTCA CTCCTTCCGA GGCGCGTACG GGCTGGCCAT GAAGGTGTCT 
TCGCCACCTC CAACCATCAT GCAGCAGAAT AAAAAAGGAG ACATGACCCA TGAGCTGGTC 
AGGCATTTTC TGATAGAGAC TGGCCCCAGA GGAGTCAAGC TCAAGGGCTG CCCCAATGAG 
CCAAACTTCG GATCGCTGTC TGCCCTGGTC TACCAGCACT CCATCATCCC ATTGGCCCTG 
CCTTGCAAGC TGGTCATTCC AAACCGAGAC CCCACAGATG AATCGAAAGA TAGCTCCGGC 
CCTGCCAACT CAACTGCAGA CCTGCTGAAA CAAGGGGCAG CCTGCAATGT GCTCTTCGTC 
AACTCTGTGG ACATGGAGTC ACTCACTGGG CCACAGGCCA TCTCTAAAGC CACATCTGAG 
ACGTTGGCTG CAGACCCCAC GCCAGCTGCC ACCATCGTTC ACTTCAAAGT CTCTGCCCAG 
GGAATCACTC TGACTGACAA CCAGAGAAAG CTCTTTTTCA GACGCCACTA CCCTCTCAAC 
ACTGTCACCT TCTGTGACCT GGATCCACAG GAAAGAAAGT GGATGAAAAC AGAGGGTGGT 
GCCCCTGCTA AGCTCTTCGG CTTCGTGGCC CGGAAGCAGG GCAGCACCAC GGACAACGCC 
TGCCACCTCT TTGCTGAGCT TGACCCCAAC CAGCCGGCCT CTGCCATCGT CAACTTCGTC 
TCCAAGGTCA TGCTGAATGC CGGCCAAAAG AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_017004804.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860293.1 Homo sapiens tensin 1 (TNS1), transcript variant X4, mRNA.

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CloneID	OHu61087
Clone ID Related Accession (Same CDS sequence)	XM_017004804.1
Accession Version	XM_017004804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGCGTA CAACGCCAGG CTGCAGGGCA TCGGACACAG TGGCAGCTTC 
CCGCCCCCTC CTCTCCACCG GCTCCAATCT TCCTCCCTCT CTGGGGTACA GGCTCGGGAG 
AAGCAGCCTG CAGAGCCCCC AGCCCCTCTG CGGAGGCGGG CGGCCAGTGA TGGACAGTAT 
GAGAACCAGT CTCCAGAAGC CACATCCCCT CGTAGCCCTG GGGTTCGCTC CCCTGTCCAG 
TGTGTCTCCC CGGAGCTGGC TCTTACCATC GCTCTCAATC CTGGAGGGCG GCCCAAAGAG 
CCCCATTTGC ACAGCTACAA GGAGGCCTTC GAGGAGATGG AGGGAACCTC CCCGAGCAGC 
CCACCACCCA GTGGGGTGCG GTCCCCCCCG GGTCTGGCCA AGACACCCCT GTCTGCTCTG 
GGCCTGAAAC CTCACAACCC AGCGGACATC CTGTTGCACC CCACAGGAGT TACCAGAAGA 
CGGATCCAGC CAGAGGAAGA TGAGGGGAAG GTGGTGGTCA GGCTGTCAGA AGAGCCCCGG 
AGCTATGTGG AGTCTGTGGC ACGGACAGCG GTGGCTGGAC CCCGAGCTCA GGACTCTGAG 
CCCAAGAGCT TTAGTGCTCC AGCCACCCAG GCCTATGGCC ATGAGATACC CCTGAGGAAC 
GGGACCCTGG GTGGCTCCTT TGTCTCCCCC AGCCCCCTCT CCACCAGCAG CCCCATCCTC 
AGTGCTGACA GCACTTCAGT GGGGAGTTTC CCGTCGGGAG AGAGCAGTGA CCAGGGTCCC 
CGGACGCCCA CCCAGCCTCT GTTGGAGTCT GGCTTCCGCT CAGGCAGCCT GGGACAGCCC 
AGCCCGTCTG CCCAGAGAAA CTACCAGAGC TCTTCTCCTC TCCCGACTGT GGGCAGTAGC 
TACAGCAGCC CCGACTACTC ACTTCAGCAT TTCAGCTCCT CTCCGGAAAG CCAGGCTCGA 
GCTCAGTTCA GTGTGGCTGG CGTCCACACG GTGCCTGGGA GCCCTCAGGC GCGCCACAGA 
ACAGTGGGCA CCAACACTCC CCCTAGTCCT GGCTTCGGCT GGCGGGCCAT CAATCCCAGC 
ATGGCTGCCC CCAGCAGTCC CAGTTTGAGC CATCACCAGA TGATGGGTCC ACCAGGCACT 
GGCTTCCATG GTAGCACTGT CTCCAGCCCC CAGAGCAGTG CAGCGACCAC CCCGGGGAGC 
CCCAGCCTGT GTCGGCACCC AGCAGGGGTC TACCAGGTTT CTGGCCTCCA CAACAAAGTG 
GCCACCACCC CGGGGAGTCC CAGCCTGGGC CGGCACCCTG GGGCTCACCA AGGCAACCTG 
GCCTCCGGTC TTCATAGCAA TGCAATAGCC AGCCCTGGAA GCCCCAGCCT GGGCCGTCAC 
CTCGGAGGGT CTGGATCTGT GGTTCCCGGC AGCCCCTGCT TGGACCGGCA TGTGGCCTAT 
GGTGGCTATT CTACCCCGGA GGATCGGAGA CCCACACTGT CCCGGCAGAG CAGTGCCTCT 
GGCTACCAGG CTCCTTCCAC GCCCTCCTTC CCTGTCTCCC CTGCCTACTA CCCTGGCCTG 
AGCAGCCCTG CCACCTCCCC GTCACCAGAC TCCGCAGCCT TCCGGCAAGG GAGCCCAACA 
CCAGCCTTGC CAGAGAAGCG AAGGATGTCA GTGGGAGACC GGGCAGGCAG CCTCCCCAAC 
TATGCCACCA TCAATGGGAA GGTGTCTTCG CCTGTCGCCA GCGGCATGTC CAGTCCCAGT 
GGGGGCAGCA CCGTCTCCTT CTCCCACACT CTGCCCGACT TCTCCAAGTA CTCCATGCCA 
GACAACAGCC CGGAGACGCG GGCTAAAGTG AAGTTTGTCC AGGACACTTC TAAGTATTGG 
TACAAGCCTG AGATCTCCAG GGAGCAGGCC ATCGCGCTCC TCAAGGACCA GGAGCCGGGG 
GCCTTCATCA TCCGCGACAG TCACTCCTTC CGAGGCGCGT ACGGGCTGGC CATGAAGGTG 
TCTTCGCCAC CTCCAACCAT CATGCAGCAG AATAAAAAAG GAGACATGAC CCATGAGCTG 
GTCAGGCATT TTCTGATAGA GACTGGCCCC AGAGGAGTCA AGCTCAAGGG CTGCCCCAAT 
GAGCCAAACT TCGGATCGCT GTCTGCCCTG GTCTACCAGC ACTCCATCAT CCCATTGGCC 
CTGCCTTGCA AGCTGGTCAT TCCAAACCGA GACCCCACAG ATGAATCGAA AGATAGCTCC 
GGCCCTGCCA ACTCAACTGC AGACCTGCTG AAACAAGGGG CAGCCTGCAA TGTGCTCTTC 
GTCAACTCTG TGGACATGGA GTCACTCACT GGGCCACAGG CCATCTCTAA AGCCACATCT 
GAGACGTTGG CTGCAGACCC CACGCCAGCT GCCACCATCG TTCACTTCAA AGTCTCTGCC 
CAGGGAATCA CTCTGACTGA CAACCAGAGA AAGCTCTTTT TCAGACGCCA CTACCCTCTC 
AACACTGTCA CCTTCTGTGA CCTGGATCCA CAGGAAAGAA AGTGGATGAA AACAGAGGGT 
GGTGCCCCTG CTAAGCTCTT CGGCTTCGTG GCCCGGAAGC AGGGCAGCAC CACGGACAAC 
GCCTGCCACC TCTTTGCTGA GCTTGACCCC AACCAGCCGG CCTCTGCCAT CGTCAACTTC 
GTCTCCAAGG TCATGCTGAA TGCCGGCCAA AAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860293.1
CDS	55..5613
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCINGNKQNTNSQSIGSISEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_017004804.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004804.1

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGGGGCAG TGAGATCAGA TTTTGATATG 
AAAGCTCAAA GCTTCTCAGC TCTCTCCTTC TTCCCTGCCT TCCTCTCTCT GTCTGCACAT 
TGCTTGAGAA CCTTCGAGAA GCAGATGGGC GCTCCTCATG TGATGCAAAG GCAGAAAGGC 
CCAAGGGTGG TGCCCTGGCT TCTGTCTGCC ATGCAGGCCT GTGCTTCATG GTTTGGTGGC 
CTCTGCATAA ATGGGAACAA ACAGAATACC AATAGCCAGT CAATTGGAAG CATCTCAGAG 
GTGGTGGGAC ACACGCAGGG GCCACTAGAT GGGAGCCTGT ATGCTAAGGT GAAGAAGAAA 
GACTCCCTGC ACGGCAGCAC CGGGGCTGTT AATGCCACAC GTCCTACACT GTCGGCCACC 
CCCAACCACG TGGAACACAC GCTTTCTGTG AGCAGCGACT CGGGCAACTC CACAGCCTCC 
ACCAAGACCG ACAAGACCGA CGAGCCTGTC CCCGGGGCCT CCAGTGCCAC TGCTGCCTTG 
AGTCCCCAGG AGAAGCGGGA GCTGGACCGC CTGCTGAGTG GCTTTGGCTT AGAGCGAGAG 
AAGCAAGGCG CCATGTACCA CACCCAGCAC CTCAGGTCCC GCCCAGCAGG GGGCTCGGCT 
GTGCCCTCCT CTGGACGCCA CGTTGTCCCA GCCCAGGTTC ATGTCAATGG TGGGGCGTTA 
GCATCTGAGC GGGAGACAGA CATCCTGGAC GATGAATTGC CAAACCAGGA TGGTCACAGT 
GCGGGCAGCA TGGGCACACT CTCTTCTCTG GACGGGGTCA CCAACACCAG TGAGGGGGGC 
TACCCAGAGG CCCTGTCCCC ACTGACCAAC GGTCTGGACA AGTCCTACCC CATGGAGCCT 
ATGGTCAATG GAGGAGGCTA CCCCTACGAG TCTGCCAGCC GGGCGGGGCC TGCCCATGCT 
GGCCACACGG CCCCCATGCG GCCCTCCTAC TCTGCACAGG AGGGTTTAGC TGGCTACCAG 
AGGGAGGGGC CCCACCCAGC CTGGCCACAG CCAGTGACCA CCTCCCACTA TGCCCATGAC 
CCCAGCGGTA TGTTCCGCTC TCAATCCTTT TCGGAAGCTG AACCCCAGCT GCCCCCAGCT 
CCGGTCCGAG GGGGAAGCAG CCGGGAGGCT GTGCAAAGGG GACTGAATTC GTGGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCACGCC AGCAGGAAAG AGCCCACTTG 
GAGAGTCTTG TAGCCAGCAG GCCCAGCCCT CAGCCATTGG CAGAGACCCC CATCCCCAGT 
CTCCCTGAGT TCCCGCGAGC AGCCTCCCAG CAGGAGATTG AACAGTCCAT CGAAACACTC 
AATATGCTGA TGCTGGACCT GGAGCCAGCC TCCGCTGCTG CCCCACTACA CAAGTCCCAG 
AGTGTCCCCG GGGCCTGGCC AGGGGCTTCT CCACTCTCCT CCCAGCCCCT CTCTGGATCC 
TCCCGTCAGT CCCATCCACT GACCCAGTCC AGATCTGGCT ATATCCCCAG TGGGCATTCG 
TTGGGAACCC CTGAGCCAGC CCCACGGGCC TCTCTGGAGT CTGTCCCTCC TGGCAGGTCT 
TACTCACCTT ATGACTATCA GCCATGTTTG GCTGGGCCTA ACCAGGATTT CCATTCAAAG 
AGCCCAGCCT CTTCCTCCTT GCCTGCCTTC CTTCCGACCA CCCACAGCCC TCCAGGGCCT 
CAGCAACCCC CAGCCTCTCT CCCTGGCCTC ACTGCTCAGC CTCTGCTCTC ACCAAAGGAA 
GCGACTTCAG ACCCCTCCCG GACTCCAGAG GAGGAGCCAT TGAATTTAGA AGGGCTGGTG 
GCCCACAGGG TAGCAGCGTA CAACGCCAGG CTGCAGGGCA TCGGACACAG TGGCAGCTTC 
CCGCCCCCTC CTCTCCACCG GCTCCAATCT TCCTCCCTCT CTGGGGTACA GGCTCGGGAG 
AAGCAGCCTG CAGAGCCCCC AGCCCCTCTG CGGAGGCGGG CGGCCAGTGA TGGACAGTAT 
GAGAACCAGT CTCCAGAAGC CACATCCCCT CGTAGCCCTG GGGTTCGCTC CCCTGTCCAG 
TGTGTCTCCC CGGAGCTGGC TCTTACCATC GCTCTCAATC CTGGAGGGCG GCCCAAAGAG 
CCCCATTTGC ACAGCTACAA GGAGGCCTTC GAGGAGATGG AGGGAACCTC CCCGAGCAGC 
CCACCACCCA GTGGGGTGCG GTCCCCCCCG GGTCTGGCCA AGACACCCCT GTCTGCTCTG 
GGCCTGAAAC CTCACAACCC AGCGGACATC CTGTTGCACC CCACAGGAGT TACCAGAAGA 
CGGATCCAGC CAGAGGAAGA TGAGGGGAAG GTGGTGGTCA GGCTGTCAGA AGAGCCCCGG 
AGCTATGTGG AGTCTGTGGC ACGGACAGCG GTGGCTGGAC CCCGAGCTCA GGACTCTGAG 
CCCAAGAGCT TTAGTGCTCC AGCCACCCAG GCCTATGGCC ATGAGATACC CCTGAGGAAC 
GGGACCCTGG GTGGCTCCTT TGTCTCCCCC AGCCCCCTCT CCACCAGCAG CCCCATCCTC 
AGTGCTGACA GCACTTCAGT GGGGAGTTTC CCGTCGGGAG AGAGCAGTGA CCAGGGTCCC 
CGGACGCCCA CCCAGCCTCT GTTGGAGTCT GGCTTCCGCT CAGGCAGCCT GGGACAGCCC 
AGCCCGTCTG CCCAGAGAAA CTACCAGAGC TCTTCTCCTC TCCCGACTGT GGGCAGTAGC 
TACAGCAGCC CCGACTACTC ACTTCAGCAT TTCAGCTCCT CTCCGGAAAG CCAGGCTCGA 
GCTCAGTTCA GTGTGGCTGG CGTCCACACG GTGCCTGGGA GCCCTCAGGC GCGCCACAGA 
ACAGTGGGCA CCAACACTCC CCCTAGTCCT GGCTTCGGCT GGCGGGCCAT CAATCCCAGC 
ATGGCTGCCC CCAGCAGTCC CAGTTTGAGC CATCACCAGA TGATGGGTCC ACCAGGCACT 
GGCTTCCATG GTAGCACTGT CTCCAGCCCC CAGAGCAGTG CAGCGACCAC CCCGGGGAGC 
CCCAGCCTGT GTCGGCACCC AGCAGGGGTC TACCAGGTTT CTGGCCTCCA CAACAAAGTG 
GCCACCACCC CGGGGAGTCC CAGCCTGGGC CGGCACCCTG GGGCTCACCA AGGCAACCTG 
GCCTCCGGTC TTCATAGCAA TGCAATAGCC AGCCCTGGAA GCCCCAGCCT GGGCCGTCAC 
CTCGGAGGGT CTGGATCTGT GGTTCCCGGC AGCCCCTGCT TGGACCGGCA TGTGGCCTAT 
GGTGGCTATT CTACCCCGGA GGATCGGAGA CCCACACTGT CCCGGCAGAG CAGTGCCTCT 
GGCTACCAGG CTCCTTCCAC GCCCTCCTTC CCTGTCTCCC CTGCCTACTA CCCTGGCCTG 
AGCAGCCCTG CCACCTCCCC GTCACCAGAC TCCGCAGCCT TCCGGCAAGG GAGCCCAACA 
CCAGCCTTGC CAGAGAAGCG AAGGATGTCA GTGGGAGACC GGGCAGGCAG CCTCCCCAAC 
TATGCCACCA TCAATGGGAA GGTGTCTTCG CCTGTCGCCA GCGGCATGTC CAGTCCCAGT 
GGGGGCAGCA CCGTCTCCTT CTCCCACACT CTGCCCGACT TCTCCAAGTA CTCCATGCCA 
GACAACAGCC CGGAGACGCG GGCTAAAGTG AAGTTTGTCC AGGACACTTC TAAGTATTGG 
TACAAGCCTG AGATCTCCAG GGAGCAGGCC ATCGCGCTCC TCAAGGACCA GGAGCCGGGG 
GCCTTCATCA TCCGCGACAG TCACTCCTTC CGAGGCGCGT ACGGGCTGGC CATGAAGGTG 
TCTTCGCCAC CTCCAACCAT CATGCAGCAG AATAAAAAAG GAGACATGAC CCATGAGCTG 
GTCAGGCATT TTCTGATAGA GACTGGCCCC AGAGGAGTCA AGCTCAAGGG CTGCCCCAAT 
GAGCCAAACT TCGGATCGCT GTCTGCCCTG GTCTACCAGC ACTCCATCAT CCCATTGGCC 
CTGCCTTGCA AGCTGGTCAT TCCAAACCGA GACCCCACAG ATGAATCGAA AGATAGCTCC 
GGCCCTGCCA ACTCAACTGC AGACCTGCTG AAACAAGGGG CAGCCTGCAA TGTGCTCTTC 
GTCAACTCTG TGGACATGGA GTCACTCACT GGGCCACAGG CCATCTCTAA AGCCACATCT 
GAGACGTTGG CTGCAGACCC CACGCCAGCT GCCACCATCG TTCACTTCAA AGTCTCTGCC 
CAGGGAATCA CTCTGACTGA CAACCAGAGA AAGCTCTTTT TCAGACGCCA CTACCCTCTC 
AACACTGTCA CCTTCTGTGA CCTGGATCCA CAGGAAAGAA AGTGGATGAA AACAGAGGGT 
GGTGCCCCTG CTAAGCTCTT CGGCTTCGTG GCCCGGAAGC AGGGCAGCAC CACGGACAAC 
GCCTGCCACC TCTTTGCTGA GCTTGACCCC AACCAGCCGG CCTCTGCCAT CGTCAACTTC 
GTCTCCAAGG TCATGCTGAA TGCCGGCCAA AAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_011511723.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510025.1 Homo sapiens tensin 1 (TNS1), transcript variant X19, mRNA.

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CloneID	OHu61088
Clone ID Related Accession (Same CDS sequence)	XM_011511723.2 , NM_001308023.1
Accession Version	XM_011511723.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511723.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGGGGTACAG 
GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCCCCTCTGC GGAGGCGGGC GGCCAGTGAT 
GGACAGTATG AGAACCAGTC TCCAGAAGCC ACATCCCCTC GTAGCCCTGG GGTTCGCTCC 
CCTGTCCAGT GTGTCTCCCC GGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGG 
CCCAAAGAGC CCCATTTGCA CAGCTACAAG GAGGCCTTCG AGGAGATGGA GGGAACCTCC 
CCGAGCAGCC CACCACCCAG TGGGGTGCGG TCCCCCCCGG GTCTGGCCAA GACACCCCTG 
TCTGCTCTGG GCCTGAAACC TCACAACCCA GCGGACATCC TGTTGCACCC CACAGGAGAG 
CCCCGGAGCT ATGTGGAGTC TGTGGCACGG ACAGCGGTGG CTGGACCCCG AGCTCAGGAC 
TCTGAGCCCA AGAGCTTTAG TGCTCCAGCC ACCCAGGCCT ATGGCCATGA GATACCCCTG 
AGGAACGGGA CCCTGGGTGG CTCCTTTGTC TCCCCCAGCC CCCTCTCCAC CAGCAGCCCC 
ATCCTCAGTG CTGACAGCAC TTCAGTGGGG AGTTTCCCGT CGGGAGAGAG CAGTGACCAG 
GGTCCCCGGA CGCCCACCCA GCCTCTGTTG GAGTCTGGCT TCCGCTCAGG CAGCCTGGGA 
CAGCCCAGCC CGTCTGCCCA GAGAAACTAC CAGAGCTCTT CTCCTCTCCC GACTGTGGGC 
AGTAGCTACA GCAGCCCCGA CTACTCACTT CAGCATTTCA GCTCCTCTCC GGAAAGCCAG 
GCTCGAGCTC AGTTCAGTGT GGCTGGCGTC CACACGGTGC CTGGGAGCCC TCAGGCGCGC 
CACAGAACAG TGGGCACCAA CACTCCCCCT AGTCCTGGCT TCGGCTGGCG GGCCATCAAT 
CCCAGCATGG CTGCCCCCAG CAGTCCCAGT TTGAGCCATC ACCAGATGAT GGGTCCACCA 
GGCACTGGCT TCCATGGTAG CACTGTCTCC AGCCCCCAGA GCAGTGCAGC GACCACCCCG 
GGGAGCCCCA GCCTGTGTCG GCACCCAGCA GGGGTCTACC AGGTTTCTGG CCTCCACAAC 
AAAGTGGCCA CCACCCCGGG GAGTCCCAGC CTGGGCCGGC ACCCTGGGGC TCACCAAGGC 
AACCTGGCCT CCGGTCTTCA TAGCAATGCA ATAGCCAGCC CTGGAAGCCC CAGCCTGGGC 
CGTCACCTCG GAGGGTCTGG ATCTGTGGTT CCCGGCAGCC CCTGCTTGGA CCGGCATGTG 
GCCTATGGTG GCTATTCTAC CCCGGAGGAT CGGAGACCCA CACTGTCCCG GCAGAGCAGT 
GCCTCTGGCT ACCAGGCTCC TTCCACGCCC TCCTTCCCTG TCTCCCCTGC CTACTACCCT 
GGCCTGAGCA GCCCTGCCAC CTCCCCGTCA CCAGACTCCG CAGCCTTCCG GCAAGGGAGC 
CCAACACCAG CCTTGCCAGA GAAGCGAAGG ATGTCAGTGG GAGACCGGGC AGGCAGCCTC 
CCCAACTATG CCACCATCAA TGGGAAGGTG TCTTCGCCTG TCGCCAGCGG CATGTCCAGT 
CCCAGTGGGG GCAGCACCGT CTCCTTCTCC CACACTCTGC CCGACTTCTC CAAGTACTCC 
ATGCCAGACA ACAGCCCGGA GACGCGGGCT AAAGTGAAGT TTGTCCAGGA CACTTCTAAG 
TATTGGTACA AGCCTGAGAT CTCCAGGGAG CAGGCCATCG CGCTCCTCAA GGACCAGGAG 
CCGGGGGCCT TCATCATCCG CGACAGTCAC TCCTTCCGAG GCGCGTACGG GCTGGCCATG 
AAGGTGTCTT CGCCACCTCC AACCATCATG CAGCAGAATA AAAAAGGAGA CATGACCCAT 
GAGCTGGTCA GGCATTTTCT GATAGAGACT GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC 
CCCAATGAGC CAAACTTCGG ATCGCTGTCT GCCCTGGTCT ACCAGCACTC CATCATCCCA 
TTGGCCCTGC CTTGCAAGCT GGTCATTCCA AACCGAGACC CCACAGATGA ATCGAAAGAT 
AGCTCCGGCC CTGCCAACTC AACTGCAGAC CTGCTGAAAC AAGGGGCAGC CTGCAATGTG 
CTCTTCGTCA ACTCTGTGGA CATGGAGTCA CTCACTGGGC CACAGGCCAT CTCTAAAGCC 
ACATCTGAGA CGTTGGCTGC AGACCCCACG CCAGCTGCCA CCATCGTTCA CTTCAAAGTC 
TCTGCCCAGG GAATCACTCT GACTGACAAC CAGAGAAAGC TCTTTTTCAG ACGCCACTAC 
CCTCTCAACA CTGTCACCTT CTGTGACCTG GATCCACAGG AAAGAAAGTG GATGAAAACA 
GAGGGTGGTG CCCCTGCTAA GCTCTTCGGC TTCGTGGCCC GGAAGCAGGG CAGCACCACG 
GACAACGCCT GCCACCTCTT TGCTGAGCTT GACCCCAACC AGCCGGCCTC TGCCATCGTC 
AACTTCGTCT CCAAGGTCAT GCTGAATGCC GGCCAAAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510025.1
CDS	406..5550
Translation	MSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEEVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_011511723.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511723.2

ORF Insert Sequence:

ATGAGTGTGA GCCGGACCAT GGAGGACAGC TGTGAGCTGG ACCTGGTGTA CGTCACAGAG 
AGGATCATCG CTGTCTCCTT CCCCAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC 
CGTGAGGTGG CGCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT GTTCAACCTC 
TCTGAGCGGA GACCTGACAT CACGAAGCTC CATGCCAAGG TACTGGAATT TGGCTGGCCC 
GACCTCCACA CCCCAGCCCT GGAGAAGATC TGCAGCATCT GTAAGGCCAT GGACACATGG 
CTCAATGCAG ACCCTCACAA TGTCGTTGTT CTACACAACA AGGGAAACCG AGGCAGGATA 
GGAGTTGTCA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC GGACCAGGCT 
CTGGACCGGT TTGCAATGAA GCGGTTCTAT GAGGATAAGA TTGTGCCCAT TGGCCAGCCA 
TCCCAAAGAA GGTACGTGCA TTACTTCAGT GGCCTGCTCT CCGGCTCCAT CAAAATGAAC 
AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT TGAGTCTAAA 
GGAGGATGTC GGCCATTTCT CCGCATCTAC CAGGCCATGC AACCTGTGTA CACATCTGGC 
ATCTACAACA TCCCAGGAGA CAGCCAGACT AGCGTCTGCA TCACCATCGA GCCAGGACTG 
CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGAAG CCCAGCCCGA 
GACGTCATCT TCCGTGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG GGTTGTCTTT 
GGGAAGGAGG ACCTTGATGA TGCTTTCAAA GATGATCGAT TTCCAGAGTA TGGCAAAGTG 
GAGTTTGTAT TTTCTTATGG GCCAGAGAAA ATTCAAGGCA TGGAGCACCT GGAGAACGGG 
CCGAGCGTGT CTGTGGACTA TAACACCTCC GACCCCCTCA TCCGCTGGGA CTCCTACGAC 
AACTTCAGTG GGCATCGAGA TGACGGCATG GAGGAGGTGG TGGGACACAC GCAGGGGCCA 
CTAGATGGGA GCCTGTATGC TAAGGTGAAG AAGAAAGACT CCCTGCACGG CAGCACCGGG 
GCTGTTAATG CCACACGTCC TACACTGTCG GCCACCCCCA ACCACGTGGA ACACACGCTT 
TCTGTGAGCA GCGACTCGGG CAACTCCACA GCCTCCACCA AGACCGACAA GACCGACGAG 
CCTGTCCCCG GGGCCTCCAG TGCCACTGCT GCCTTGAGTC CCCAGGAGAA GCGGGAGCTG 
GACCGCCTGC TGAGTGGCTT TGGCTTAGAG CGAGAGAAGC AAGGCGCCAT GTACCACACC 
CAGCACCTCA GGTCCCGCCC AGCAGGGGGC TCGGCTGTGC CCTCCTCTGG ACGCCACGTT 
GTCCCAGCCC AGGTTCATGT CAATGGTGGG GCGTTAGCAT CTGAGCGGGA GACAGACATC 
CTGGACGATG AATTGCCAAA CCAGGATGGT CACAGTGCGG GCAGCATGGG CACACTCTCT 
TCTCTGGACG GGGTCACCAA CACCAGTGAG GGGGGCTACC CAGAGGCCCT GTCCCCACTG 
ACCAACGGTC TGGACAAGTC CTACCCCATG GAGCCTATGG TCAATGGAGG AGGCTACCCC 
TACGAGTCTG CCAGCCGGGC GGGGCCTGCC CATGCTGGCC ACACGGCCCC CATGCGGCCC 
TCCTACTCTG CACAGGAGGG TTTAGCTGGC TACCAGAGGG AGGGGCCCCA CCCAGCCTGG 
CCACAGCCAG TGACCACCTC CCACTATGCC CATGACCCCA GCGGTATGTT CCGCTCTCAA 
TCCTTTTCGG AAGCTGAACC CCAGCTGCCC CCAGCTCCGG TCCGAGGGGG AAGCAGCCGG 
GAGGCTGTGC AAAGGGGACT GAATTCGTGG CAGCAGCAGC AGCAGCAGCA GCAGCAGCCT 
CGCCCACCTC CACGCCAGCA GGAAAGAGCC CACTTGGAGA GTCTTGTAGC CAGCAGGCCC 
AGCCCTCAGC CATTGGCAGA GACCCCCATC CCCAGTCTCC CTGAGTTCCC GCGAGCAGCC 
TCCCAGCAGG AGATTGAACA GTCCATCGAA ACACTCAATA TGCTGATGCT GGACCTGGAG 
CCAGCCTCCG CTGCTGCCCC ACTACACAAG TCCCAGAGTG TCCCCGGGGC CTGGCCAGGG 
GCTTCTCCAC TCTCCTCCCA GCCCCTCTCT GGATCCTCCC GTCAGTCCCA TCCACTGACC 
CAGTCCAGAT CTGGCTATAT CCCCAGTGGG CATTCGTTGG GAACCCCTGA GCCAGCCCCA 
CGGGCCTCTC TGGAGTCTGT CCCTCCTGGC AGGTCTTACT CACCTTATGA CTATCAGCCA 
TGTTTGGCTG GGCCTAACCA GGATTTCCAT TCAAAGAGCC CAGCCTCTTC CTCCTTGCCT 
GCCTTCCTTC CGACCACCCA CAGCCCTCCA GGGCCTCAGC AACCCCCAGC CTCTCTCCCT 
GGCCTCACTG CTCAGCCTCT GCTCTCACCA AAGGAAGCGA CTTCAGACCC CTCCCGGACT 
CCAGAGGAGG AGCCATTGAA TTTAGAAGGG CTGGTGGCCC ACAGGGTAGC AGGGGTACAG 
GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCCCCTCTGC GGAGGCGGGC GGCCAGTGAT 
GGACAGTATG AGAACCAGTC TCCAGAAGCC ACATCCCCTC GTAGCCCTGG GGTTCGCTCC 
CCTGTCCAGT GTGTCTCCCC GGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGG 
CCCAAAGAGC CCCATTTGCA CAGCTACAAG GAGGCCTTCG AGGAGATGGA GGGAACCTCC 
CCGAGCAGCC CACCACCCAG TGGGGTGCGG TCCCCCCCGG GTCTGGCCAA GACACCCCTG 
TCTGCTCTGG GCCTGAAACC TCACAACCCA GCGGACATCC TGTTGCACCC CACAGGAGAG 
CCCCGGAGCT ATGTGGAGTC TGTGGCACGG ACAGCGGTGG CTGGACCCCG AGCTCAGGAC 
TCTGAGCCCA AGAGCTTTAG TGCTCCAGCC ACCCAGGCCT ATGGCCATGA GATACCCCTG 
AGGAACGGGA CCCTGGGTGG CTCCTTTGTC TCCCCCAGCC CCCTCTCCAC CAGCAGCCCC 
ATCCTCAGTG CTGACAGCAC TTCAGTGGGG AGTTTCCCGT CGGGAGAGAG CAGTGACCAG 
GGTCCCCGGA CGCCCACCCA GCCTCTGTTG GAGTCTGGCT TCCGCTCAGG CAGCCTGGGA 
CAGCCCAGCC CGTCTGCCCA GAGAAACTAC CAGAGCTCTT CTCCTCTCCC GACTGTGGGC 
AGTAGCTACA GCAGCCCCGA CTACTCACTT CAGCATTTCA GCTCCTCTCC GGAAAGCCAG 
GCTCGAGCTC AGTTCAGTGT GGCTGGCGTC CACACGGTGC CTGGGAGCCC TCAGGCGCGC 
CACAGAACAG TGGGCACCAA CACTCCCCCT AGTCCTGGCT TCGGCTGGCG GGCCATCAAT 
CCCAGCATGG CTGCCCCCAG CAGTCCCAGT TTGAGCCATC ACCAGATGAT GGGTCCACCA 
GGCACTGGCT TCCATGGTAG CACTGTCTCC AGCCCCCAGA GCAGTGCAGC GACCACCCCG 
GGGAGCCCCA GCCTGTGTCG GCACCCAGCA GGGGTCTACC AGGTTTCTGG CCTCCACAAC 
AAAGTGGCCA CCACCCCGGG GAGTCCCAGC CTGGGCCGGC ACCCTGGGGC TCACCAAGGC 
AACCTGGCCT CCGGTCTTCA TAGCAATGCA ATAGCCAGCC CTGGAAGCCC CAGCCTGGGC 
CGTCACCTCG GAGGGTCTGG ATCTGTGGTT CCCGGCAGCC CCTGCTTGGA CCGGCATGTG 
GCCTATGGTG GCTATTCTAC CCCGGAGGAT CGGAGACCCA CACTGTCCCG GCAGAGCAGT 
GCCTCTGGCT ACCAGGCTCC TTCCACGCCC TCCTTCCCTG TCTCCCCTGC CTACTACCCT 
GGCCTGAGCA GCCCTGCCAC CTCCCCGTCA CCAGACTCCG CAGCCTTCCG GCAAGGGAGC 
CCAACACCAG CCTTGCCAGA GAAGCGAAGG ATGTCAGTGG GAGACCGGGC AGGCAGCCTC 
CCCAACTATG CCACCATCAA TGGGAAGGTG TCTTCGCCTG TCGCCAGCGG CATGTCCAGT 
CCCAGTGGGG GCAGCACCGT CTCCTTCTCC CACACTCTGC CCGACTTCTC CAAGTACTCC 
ATGCCAGACA ACAGCCCGGA GACGCGGGCT AAAGTGAAGT TTGTCCAGGA CACTTCTAAG 
TATTGGTACA AGCCTGAGAT CTCCAGGGAG CAGGCCATCG CGCTCCTCAA GGACCAGGAG 
CCGGGGGCCT TCATCATCCG CGACAGTCAC TCCTTCCGAG GCGCGTACGG GCTGGCCATG 
AAGGTGTCTT CGCCACCTCC AACCATCATG CAGCAGAATA AAAAAGGAGA CATGACCCAT 
GAGCTGGTCA GGCATTTTCT GATAGAGACT GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC 
CCCAATGAGC CAAACTTCGG ATCGCTGTCT GCCCTGGTCT ACCAGCACTC CATCATCCCA 
TTGGCCCTGC CTTGCAAGCT GGTCATTCCA AACCGAGACC CCACAGATGA ATCGAAAGAT 
AGCTCCGGCC CTGCCAACTC AACTGCAGAC CTGCTGAAAC AAGGGGCAGC CTGCAATGTG 
CTCTTCGTCA ACTCTGTGGA CATGGAGTCA CTCACTGGGC CACAGGCCAT CTCTAAAGCC 
ACATCTGAGA CGTTGGCTGC AGACCCCACG CCAGCTGCCA CCATCGTTCA CTTCAAAGTC 
TCTGCCCAGG GAATCACTCT GACTGACAAC CAGAGAAAGC TCTTTTTCAG ACGCCACTAC 
CCTCTCAACA CTGTCACCTT CTGTGACCTG GATCCACAGG AAAGAAAGTG GATGAAAACA 
GAGGGTGGTG CCCCTGCTAA GCTCTTCGGC TTCGTGGCCC GGAAGCAGGG CAGCACCACG 
GACAACGCCT GCCACCTCTT TGCTGAGCTT GACCCCAACC AGCCGGCCTC TGCCATCGTC 
AACTTCGTCT CCAAGGTCAT GCTGAATGCC GGCCAAAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS1 ( XM_024453079.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308847.1 Homo sapiens tensin 1 (TNS1), transcript variant X12, mRNA.

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CloneID	OHu104946
Clone ID Related Accession (Same CDS sequence)	XM_024453079.1
Accession Version	XM_024453079.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tensin-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTGGA TCTGTCTGTC CTGCATGCTC TGGCCAGAGG ATCTGGAGGC CCCCAAGACA 
CACCGCTTCA AGGTGAAGAC CTTCAAGAAG GTGAAGCCCT GTGGGATCTG CCGCCAGGTC 
ATCACCCAGG AAGGCTGCAC CTGCAAAGTC TGCAGCTTCT CCTGTCATCG GAAATGCCAG 
GCCAAGGTGG CTGCCCCCTG CGTTCCTCCA TCCAACCATG AGCTGGTGCC CATCACCACT 
GAGAATGCAC CAAAGAATGT AGTGGACAAG GGAGAAGGAG CCTCCCGGGG TGGAAACACA 
CGGAAAAGCC TCGAGGACAA CGGCTCCACC AGGGTCACCC CGAGTGTCCA GCCCCACCTC 
CAGCCCATCA GAAACATGAG TGTGAGCCGG ACCATGGAGG ACAGCTGTGA GCTGGACCTG 
GTGTACGTCA CAGAGAGGAT CATCGCTGTC TCCTTCCCCA GCACAGCCAA TGAGGAGAAC 
TTCCGGAGCA ACCTCCGTGA GGTGGCGCAG ATGCTCAAGT CCAAACATGG AGGCAACTAC 
CTGCTGTTCA ACCTCTCTGA GCGGAGACCT GACATCACGA AGCTCCATGC CAAGGTACTG 
GAATTTGGCT GGCCCGACCT CCACACCCCA GCCCTGGAGA AGATCTGCAG CATCTGTAAG 
GCCATGGACA CATGGCTCAA TGCAGACCCT CACAATGTCG TTGTTCTACA CAACAAGGGA 
AACCGAGGCA GGATAGGAGT TGTCATCGCG GCTTACATGC ACTACAGCAA CATTTCTGCC 
AGTGCGGACC AGGCTCTGGA CCGGTTTGCA ATGAAGCGGT TCTATGAGGA TAAGATTGTG 
CCCATTGGCC AGCCATCCCA AAGAAGGTAC GTGCATTACT TCAGTGGCCT GCTCTCCGGC 
TCCATCAAAA TGAACAACAA GCCCTTGTTT CTGCACCACG TGATCATGCA CGGCATCCCC 
AACTTTGAGT CTAAAGGAGG ATGTCGGCCA TTTCTCCGCA TCTACCAGGC CATGCAACCT 
GTGTACACAT CTGGCATCTA CAACATCCCA GGAGACAGCC AGACTAGCGT CTGCATCACC 
ATCGAGCCAG GACTGCTCTT GAAGGGAGAC ATCTTGCTGA AGTGCTACCA CAAGAAGTTC 
CGAAGCCCAG CCCGAGACGT CATCTTCCGT GTGCAGTTCC ACACCTGTGC CATCCATGAC 
CTGGGGGTTG TCTTTGGGAA GGAGGACCTT GATGATGCTT TCAAAGATGA TCGATTTCCA 
GAGTATGGCA AAGTGGAGTT TGTATTTTCT TATGGGCCAG AGAAAATTCA AGGCATGGAG 
CACCTGGAGA ACGGGCCGAG CGTGTCTGTG GACTATAACA CCTCCGACCC CCTCATCCGC 
TGGGACTCCT ACGACAACTT CAGTGGGCAT CGAGATGACG GCATGGAGGG GGCAGTGAGA 
TCAGATTTTG ATATGAAAGC TCAAAGCTTC TCAGCTCTCT CCTTCTTCCC TGCCTTCCTC 
TCTCTGTCTG CACATTGCTT GAGAACCTTC GAGAAGCAGA TGGGCGCTCC TCATGTGATG 
CAAAGGCAGA AAGGCCCAAG GGTGGTGCCC TGGCTTCTGT CTGCCATGCA GGCCTGTGCT 
TCATGGTTTG GTGGCCTCTG CATAAAGGTG GTGGGACACA CGCAGGGGCC ACTAGATGGG 
AGCCTGTATG CTAAGGTGAA GAAGAAAGAC TCCCTGCACG GCAGCACCGG GGCTGTTAAT 
GCCACACGTC CTACACTGTC GGCCACCCCC AACCACGTGG AACACACGCT TTCTGTGAGC 
AGCGACTCGG GCAACTCCAC AGCCTCCACC AAGACCGACA AGACCGACGA GCCTGTCCCC 
GGGGCCTCCA GTGCCACTGC TGCCTTGAGT CCCCAGGAGA AGCGGGAGCT GGACCGCCTG 
CTGAGTGGCT TTGGCTTAGA GCGAGAGAAG CAAGGCGCCA TGTACCACAC CCAGCACCTC 
AGGTCCCGCC CAGCAGGGGG CTCGGCTGTG CCCTCCTCTG GACGCCACGT TGTCCCAGCC 
CAGGTTCATG TCAATGGTGG GGCGTTAGCA TCTGAGCGGG AGACAGACAT CCTGGACGAT 
GAATTGCCAA ACCAGGATGG TCACAGTGCG GGCAGCATGG GCACACTCTC TTCTCTGGAC 
GGGGTCACCA ACACCAGTGA GGGGGGCTAC CCAGAGGCCC TGTCCCCACT GACCAACGGT 
CTGGACAAGT CCTACCCCAT GGAGCCTATG GTCAATGGAG GAGGCTACCC CTACGAGTCT 
GCCAGCCGGG CGGGGCCTGC CCATGCTGGC CACACGGCCC CCATGCGGCC CTCCTACTCT 
GCACAGGAGG GTTTAGCTGG CTACCAGAGG GAGGGGCCCC ACCCAGCCTG GCCACAGCCA 
GTGACCACCT CCCACTATGC CCATGACCCC AGCGGTATGT TCCGCTCTCA ATCCTTTTCG 
GAAGCTGAAC CCCAGCTGCC CCCAGCTCCG GTCCGAGGGG GAAGCAGCCG GGAGGCTGTG 
CAAAGGGGAC TGAATTCGTG GCAGCAGCAG CAGCAGCAGC AGCAGCAGCC TCGCCCACCT 
CCACGCCAGC AGGAAAGAGC CCACTTGGAG AGTCTTGTAG CCAGCAGGCC CAGCCCTCAG 
CCATTGGCAG AGACCCCCAT CCCCAGTCTC CCTGAGTTCC CGCGAGCAGC CTCCCAGCAG 
GAGATTGAAC AGTCCATCGA AACACTCAAT ATGCTGATGC TGGACCTGGA GCCAGCCTCC 
GCTGCTGCCC CACTACACAA GTCCCAGAGT GTCCCCGGGG CCTGGCCAGG GGCTTCTCCA 
CTCTCCTCCC AGCCCCTCTC TGGATCCTCC CGTCAGTCCC ATCCACTGAC CCAGTCCAGA 
TCTGGCTATA TCCCCAGTGG GCATTCGTTG GGAACCCCTG AGCCAGCCCC ACGGGCCTCT 
CTGGAGTCTG TCCCTCCTGG CAGGTCTTAC TCACCTTATG ACTATCAGCC ATGTTTGGCT 
GGGCCTAACC AGGATTTCCA TTCAAAGAGC CCAGCCTCTT CCTCCTTGCC TGCCTTCCTT 
CCGACCACCC ACAGCCCTCC AGGGCCTCAG CAACCCCCAG CCTCTCTCCC TGGCCTCACT 
GCTCAGCCTC TGCTCTCACC AAAGGAAGCG ACTTCAGACC CCTCCCGGAC TCCAGAGGAG 
GAGCCATTGA ATTTAGAAGG GCTGGTGGCC CACAGGGTAG CAGCGTACAA CGCCAGGCTG 
CAGGGCATCG GACACAGTGG CAGCTTCCCG CCCCCTCCTC TCCACCGGCT CCAATCTTCC 
TCCCTCTCTG GGGTACAGGC TCGGGAGAAG CAGCCTGCAG AGCCCCCAGC CCCTCTGCGG 
AGGCGGGCGG CCAGTGATGG ACAGTATGAG AACCAGTCTC CAGAAGCCAC ATCCCCTCGT 
AGCCCTGGGG TTCGCTCCCC TGTCCAGTGT GTCTCCCCGG AGCTGGCTCT TACCATCGCT 
CTCAATCCTG GAGGGCGGCC CAAAGAGCCC CATTTGCACA GCTACAAGGA GGCCTTCGAG 
GAGATGGAGG GAACCTCCCC GAGCAGCCCA CCACCCAGTG GGGTGCGGTC CCCCCCGGGT 
CTGGCCAAGA CACCCCTGTC TGCTCTGGGC CTGAAACCTC ACAACCCAGC GGACATCCTG 
TTGCACCCCA CAGGAGTTAC CAGAAGACGG ATCCAGCCAG ATTCTCTTTC TCAAGAAATA 
ATCCCTGTTA ATCTCTTTCT CTCTTTTTCC TTTTTTGCTC TTCCTCATCT TCACCACCTG 
CCCTTCTTTC CTGCCTCTCC TTCCTCCCAC CTCTCTTCTC GTCTTCCCTC AGAGGAAGAT 
GAGGGGAAGG TGGTGGTCAG GCTGTCAGAA GAGCCCCGGA GCTATGTGGA GTCTGTGGCA 
CGGACAGCGG TGGCTGGACC CCGAGCTCAG GACTCTGAGC CCAAGAGCTT TAGTGCTCCA 
GCCACCCAGG CCTATGGCCA TGAGATACCC CTGAGGAACG GGACCCTGGG TGGCTCCTTT 
GTCTCCCCCA GCCCCCTCTC CACCAGCAGC CCCATCCTCA GTGCTGACAG CACTTCAGTG 
GGGAGTTTCC CGTCGGGAGA GAGCAGTGAC CAGGGTCCCC GGACGCCCAC CCAGCCTCTG 
TTGGAGTCTG GCTTCCGCTC AGGCAGCCTG GGACAGCCCA GCCCGTCTGC CCAGAGAAAC 
TACCAGAGCT CTTCTCCTCT CCCGACTGTG GGCAGTAGCT ACAGCAGCCC CGACTACTCA 
CTTCAGCATT TCAGCTCCTC TCCGGAAAGC CAGGCTCGAG CTCAGTTCAG TGTGGCTGGC 
GTCCACACGG TGCCTGGGAG CCCTCAGGCG CGCCACAGAA CAGTGGGCAC CAACACTCCC 
CCTAGTCCTG GCTTCGGCTG GCGGGCCATC AATCCCAGCA TGGCTGCCCC CAGCAGTCCC 
AGTTTGAGCC ATCACCAGAT GATGGGTCCA CCAGGCACTG GCTTCCATGG TAGCACTGTC 
TCCAGCCCCC AGAGCAGTGC AGCGACCACC CCGGGGAGCC CCAGCCTGTG TCGGCACCCA 
GCAGGGGTCT ACCAGGTTTC TGGCCTCCAC AACAAAGTGG CCACCACCCC GGGGAGTCCC 
AGCCTGGGCC GGCACCCTGG GGCTCACCAA GGCAACCTGG CCTCCGGTCT TCATAGCAAT 
GCAATAGCCA GCCCTGGAAG CCCCAGCCTG GGCCGTCACC TCGGAGGGTC TGGATCTGTG 
GTTCCCGGCA GCCCCTGCTT GGACCGGCAT GTGGCCTATG GTGGCTATTC TACCCCGGAG 
GATCGGAGAC CCACACTGTC CCGGCAGAGC AGTGCCTCTG GCTACCAGGC TCCTTCCACG 
CCCTCCTTCC CTGTCTCCCC TGCCTACTAC CCTGGCCTGA GCAGCCCTGC CACCTCCCCG 
TCACCAGACT CCGCAGCCTT CCGGCAAGGG AGCCCAACAC CAGCCTTGCC AGAGAAGCGA 
AGGATGTCAG TGGGAGACCG GGCAGGCAGC CTCCCCAACT ATGCCACCAT CAATGGGAAG 
GTGTCTTCGC CTGTCGCCAG CGGCATGTCC AGTCCCAGTG GGGGCAGCAC CGTCTCCTTC 
TCCCACACTC TGCCCGACTT CTCCAAGTAC TCCATGCCAG ACAACAGCCC GGAGACGCGG 
GCTAAAGTGA AGTTTGTCCA GGACACTTCT AAGTATTGGT ACAAGCCTGA GATCTCCAGG 
GAGCAGGCCA TCGCGCTCCT CAAGGACCAG GAGCCGGGGG CCTTCATCAT CCGCGACAGT 
CACTCCTTCC GAGGCGCGTA CGGGCTGGCC ATGAAGGTGT CTTCGCCACC TCCAACCATC 
ATGCAGCAGA ATAAAAAAGG AGACATGACC CATGAGCTGG TCAGGCATTT TCTGATAGAG 
ACTGGCCCCA GAGGAGTCAA GCTCAAGGGC TGCCCCAATG AGCCAAACTT CGGATCGCTG 
TCTGCCCTGG TCTACCAGCA CTCCATCATC CCATTGGCCC TGCCTTGCAA GCTGGTCATT 
CCAAACCGAG ACCCCACAGA TGAATCGAAA GATAGCTCCG GCCCTGCCAA CTCAACTGCA 
GACCTGCTGA AACAAGGGGC AGCCTGCAAT GTGCTCTTCG TCAACTCTGT GGACATGGAG 
TCACTCACTG GGCCACAGGC CATCTCTAAA GCCACATCTG AGACGTTGGC TGCAGACCCC 
ACGCCAGCTG CCACCATCGT TCACTTCAAA GTCTCTGCCC AGGGAATCAC TCTGACTGAC 
AACCAGAGAA AGCTCTTTTT CAGACGCCAC TACCCTCTCA ACACTGTCAC CTTCTGTGAC 
CTGGATCCAC AGGAAAGAAA GTGGATGAAA ACAGAGGGTG GTGCCCCTGC TAAGCTCTTC 
GGCTTCGTGG CCCGGAAGCA GGGCAGCACC ACGGACAACG CCTGCCACCT CTTTGCTGAG 
CTTGACCCCA ACCAGCCGGC CTCTGCCATC GTCAACTTCG TCTCCAAGGT CATGCTGAAT 
GCCGGCCAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308847.1
CDS	124..6141
Translation	MTWICLSCMLWPEDLEAPKTHRFKVKTFKKVKPCGICRQVITQEGCTCKVCSFSCHRKCQAKVAAPCVPPSNHELVPITTENAPKNVVDKGEGASRGGNTRKSLEDNGSTRVTPSVQPHLQPIRNMSVSRTMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSERRPDITKLHAKVLEFGWPDLHTPALEKICSICKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQTSVCITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDDAFKDDRFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHRDDGMEGAVRSDFDMKAQSFSALSFFPAFLSLSAHCLRTFEKQMGAPHVMQRQKGPRVVPWLLSAMQACASWFGGLCIKVVGHTQGPLDGSLYAKVKKKDSLHGSTGAVNATRPTLSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVPGASSATAALSPQEKRELDRLLSGFGLEREKQGAMYHTQHLRSRPAGGSAVPSSGRHVVPAQVHVNGGALASERETDILDDELPNQDGHSAGSMGTLSSLDGVTNTSEGGYPEALSPLTNGLDKSYPMEPMVNGGGYPYESASRAGPAHAGHTAPMRPSYSAQEGLAGYQREGPHPAWPQPVTTSHYAHDPSGMFRSQSFSEAEPQLPPAPVRGGSSREAVQRGLNSWQQQQQQQQQPRPPPRQQERAHLESLVASRPSPQPLAETPIPSLPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLSGSSRQSHPLTQSRSGYIPSGHSLGTPEPAPRASLESVPPGRSYSPYDYQPCLAGPNQDFHSKSPASSSLPAFLPTTHSPPGPQQPPASLPGLTAQPLLSPKEATSDPSRTPEEEPLNLEGLVAHRVAAYNARLQGIGHSGSFPPPPLHRLQSSSLSGVQAREKQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPPSGVRSPPGLAKTPLSALGLKPHNPADILLHPTGVTRRRIQPDSLSQEIIPVNLFLSFSFFALPHLHHLPFFPASPSSHLSSRLPSEEDEGKVVVRLSEEPRSYVESVARTAVAGPRAQDSEPKSFSAPATQAYGHEIPLRNGTLGGSFVSPSPLSTSSPILSADSTSVGSFPSGESSDQGPRTPTQPLLESGFRSGSLGQPSPSAQRNYQSSSPLPTVGSSYSSPDYSLQHFSSSPESQARAQFSVAGVHTVPGSPQARHRTVGTNTPPSPGFGWRAINPSMAAPSSPSLSHHQMMGPPGTGFHGSTVSSPQSSAATTPGSPSLCRHPAGVYQVSGLHNKVATTPGSPSLGRHPGAHQGNLASGLHSNAIASPGSPSLGRHLGGSGSVVPGSPCLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSPVASGMSSPSGGSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTIMQQNKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSIIPLALPCKLVIPNRDPTDESKDSSGPANSTADLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLNAGQKR

Target ORF information:

RefSeq Version	XM_024453079.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tensin 1 (TNS1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453079.1

ORF Insert Sequence:

ATGACGTGGA TCTGTCTGTC CTGCATGCTC TGGCCAGAGG ATCTGGAGGC CCCCAAGACA 
CACCGCTTCA AGGTGAAGAC CTTCAAGAAG GTGAAGCCCT GTGGGATCTG CCGCCAGGTC 
ATCACCCAGG AAGGCTGCAC CTGCAAAGTC TGCAGCTTCT CCTGTCATCG GAAATGCCAG 
GCCAAGGTGG CTGCCCCCTG CGTTCCTCCA TCCAACCATG AGCTGGTGCC CATCACCACT 
GAGAATGCAC CAAAGAATGT AGTGGACAAG GGAGAAGGAG CCTCCCGGGG TGGAAACACA 
CGGAAAAGCC TCGAGGACAA CGGCTCCACC AGGGTCACCC CGAGTGTCCA GCCCCACCTC 
CAGCCCATCA GAAACATGAG TGTGAGCCGG ACCATGGAGG ACAGCTGTGA GCTGGACCTG 
GTGTACGTCA CAGAGAGGAT CATCGCTGTC TCCTTCCCCA GCACAGCCAA TGAGGAGAAC 
TTCCGGAGCA ACCTCCGTGA GGTGGCGCAG ATGCTCAAGT CCAAACATGG AGGCAACTAC 
CTGCTGTTCA ACCTCTCTGA GCGGAGACCT GACATCACGA AGCTCCATGC CAAGGTACTG 
GAATTTGGCT GGCCCGACCT CCACACCCCA GCCCTGGAGA AGATCTGCAG CATCTGTAAG 
GCCATGGACA CATGGCTCAA TGCAGACCCT CACAATGTCG TTGTTCTACA CAACAAGGGA 
AACCGAGGCA GGATAGGAGT TGTCATCGCG GCTTACATGC ACTACAGCAA CATTTCTGCC 
AGTGCGGACC AGGCTCTGGA CCGGTTTGCA ATGAAGCGGT TCTATGAGGA TAAGATTGTG 
CCCATTGGCC AGCCATCCCA AAGAAGGTAC GTGCATTACT TCAGTGGCCT GCTCTCCGGC 
TCCATCAAAA TGAACAACAA GCCCTTGTTT CTGCACCACG TGATCATGCA CGGCATCCCC 
AACTTTGAGT CTAAAGGAGG ATGTCGGCCA TTTCTCCGCA TCTACCAGGC CATGCAACCT 
GTGTACACAT CTGGCATCTA CAACATCCCA GGAGACAGCC AGACTAGCGT CTGCATCACC 
ATCGAGCCAG GACTGCTCTT GAAGGGAGAC ATCTTGCTGA AGTGCTACCA CAAGAAGTTC 
CGAAGCCCAG CCCGAGACGT CATCTTCCGT GTGCAGTTCC ACACCTGTGC CATCCATGAC 
CTGGGGGTTG TCTTTGGGAA GGAGGACCTT GATGATGCTT TCAAAGATGA TCGATTTCCA 
GAGTATGGCA AAGTGGAGTT TGTATTTTCT TATGGGCCAG AGAAAATTCA AGGCATGGAG 
CACCTGGAGA ACGGGCCGAG CGTGTCTGTG GACTATAACA CCTCCGACCC CCTCATCCGC 
TGGGACTCCT ACGACAACTT CAGTGGGCAT CGAGATGACG GCATGGAGGG GGCAGTGAGA 
TCAGATTTTG ATATGAAAGC TCAAAGCTTC TCAGCTCTCT CCTTCTTCCC TGCCTTCCTC 
TCTCTGTCTG CACATTGCTT GAGAACCTTC GAGAAGCAGA TGGGCGCTCC TCATGTGATG 
CAAAGGCAGA AAGGCCCAAG GGTGGTGCCC TGGCTTCTGT CTGCCATGCA GGCCTGTGCT 
TCATGGTTTG GTGGCCTCTG CATAAAGGTG GTGGGACACA CGCAGGGGCC ACTAGATGGG 
AGCCTGTATG CTAAGGTGAA GAAGAAAGAC TCCCTGCACG GCAGCACCGG GGCTGTTAAT 
GCCACACGTC CTACACTGTC GGCCACCCCC AACCACGTGG AACACACGCT TTCTGTGAGC 
AGCGACTCGG GCAACTCCAC AGCCTCCACC AAGACCGACA AGACCGACGA GCCTGTCCCC 
GGGGCCTCCA GTGCCACTGC TGCCTTGAGT CCCCAGGAGA AGCGGGAGCT GGACCGCCTG 
CTGAGTGGCT TTGGCTTAGA GCGAGAGAAG CAAGGCGCCA TGTACCACAC CCAGCACCTC 
AGGTCCCGCC CAGCAGGGGG CTCGGCTGTG CCCTCCTCTG GACGCCACGT TGTCCCAGCC 
CAGGTTCATG TCAATGGTGG GGCGTTAGCA TCTGAGCGGG AGACAGACAT CCTGGACGAT 
GAATTGCCAA ACCAGGATGG TCACAGTGCG GGCAGCATGG GCACACTCTC TTCTCTGGAC 
GGGGTCACCA ACACCAGTGA GGGGGGCTAC CCAGAGGCCC TGTCCCCACT GACCAACGGT 
CTGGACAAGT CCTACCCCAT GGAGCCTATG GTCAATGGAG GAGGCTACCC CTACGAGTCT 
GCCAGCCGGG CGGGGCCTGC CCATGCTGGC CACACGGCCC CCATGCGGCC CTCCTACTCT 
GCACAGGAGG GTTTAGCTGG CTACCAGAGG GAGGGGCCCC ACCCAGCCTG GCCACAGCCA 
GTGACCACCT CCCACTATGC CCATGACCCC AGCGGTATGT TCCGCTCTCA ATCCTTTTCG 
GAAGCTGAAC CCCAGCTGCC CCCAGCTCCG GTCCGAGGGG GAAGCAGCCG GGAGGCTGTG 
CAAAGGGGAC TGAATTCGTG GCAGCAGCAG CAGCAGCAGC AGCAGCAGCC TCGCCCACCT 
CCACGCCAGC AGGAAAGAGC CCACTTGGAG AGTCTTGTAG CCAGCAGGCC CAGCCCTCAG 
CCATTGGCAG AGACCCCCAT CCCCAGTCTC CCTGAGTTCC CGCGAGCAGC CTCCCAGCAG 
GAGATTGAAC AGTCCATCGA AACACTCAAT ATGCTGATGC TGGACCTGGA GCCAGCCTCC 
GCTGCTGCCC CACTACACAA GTCCCAGAGT GTCCCCGGGG CCTGGCCAGG GGCTTCTCCA 
CTCTCCTCCC AGCCCCTCTC TGGATCCTCC CGTCAGTCCC ATCCACTGAC CCAGTCCAGA 
TCTGGCTATA TCCCCAGTGG GCATTCGTTG GGAACCCCTG AGCCAGCCCC ACGGGCCTCT 
CTGGAGTCTG TCCCTCCTGG CAGGTCTTAC TCACCTTATG ACTATCAGCC ATGTTTGGCT 
GGGCCTAACC AGGATTTCCA TTCAAAGAGC CCAGCCTCTT CCTCCTTGCC TGCCTTCCTT 
CCGACCACCC ACAGCCCTCC AGGGCCTCAG CAACCCCCAG CCTCTCTCCC TGGCCTCACT 
GCTCAGCCTC TGCTCTCACC AAAGGAAGCG ACTTCAGACC CCTCCCGGAC TCCAGAGGAG 
GAGCCATTGA ATTTAGAAGG GCTGGTGGCC CACAGGGTAG CAGCGTACAA CGCCAGGCTG 
CAGGGCATCG GACACAGTGG CAGCTTCCCG CCCCCTCCTC TCCACCGGCT CCAATCTTCC 
TCCCTCTCTG GGGTACAGGC TCGGGAGAAG CAGCCTGCAG AGCCCCCAGC CCCTCTGCGG 
AGGCGGGCGG CCAGTGATGG ACAGTATGAG AACCAGTCTC CAGAAGCCAC ATCCCCTCGT 
AGCCCTGGGG TTCGCTCCCC TGTCCAGTGT GTCTCCCCGG AGCTGGCTCT TACCATCGCT 
CTCAATCCTG GAGGGCGGCC CAAAGAGCCC CATTTGCACA GCTACAAGGA GGCCTTCGAG 
GAGATGGAGG GAACCTCCCC GAGCAGCCCA CCACCCAGTG GGGTGCGGTC CCCCCCGGGT 
CTGGCCAAGA CACCCCTGTC TGCTCTGGGC CTGAAACCTC ACAACCCAGC GGACATCCTG 
TTGCACCCCA CAGGAGTTAC CAGAAGACGG ATCCAGCCAG ATTCTCTTTC TCAAGAAATA 
ATCCCTGTTA ATCTCTTTCT CTCTTTTTCC TTTTTTGCTC TTCCTCATCT TCACCACCTG 
CCCTTCTTTC CTGCCTCTCC TTCCTCCCAC CTCTCTTCTC GTCTTCCCTC AGAGGAAGAT 
GAGGGGAAGG TGGTGGTCAG GCTGTCAGAA GAGCCCCGGA GCTATGTGGA GTCTGTGGCA 
CGGACAGCGG TGGCTGGACC CCGAGCTCAG GACTCTGAGC CCAAGAGCTT TAGTGCTCCA 
GCCACCCAGG CCTATGGCCA TGAGATACCC CTGAGGAACG GGACCCTGGG TGGCTCCTTT 
GTCTCCCCCA GCCCCCTCTC CACCAGCAGC CCCATCCTCA GTGCTGACAG CACTTCAGTG 
GGGAGTTTCC CGTCGGGAGA GAGCAGTGAC CAGGGTCCCC GGACGCCCAC CCAGCCTCTG 
TTGGAGTCTG GCTTCCGCTC AGGCAGCCTG GGACAGCCCA GCCCGTCTGC CCAGAGAAAC 
TACCAGAGCT CTTCTCCTCT CCCGACTGTG GGCAGTAGCT ACAGCAGCCC CGACTACTCA 
CTTCAGCATT TCAGCTCCTC TCCGGAAAGC CAGGCTCGAG CTCAGTTCAG TGTGGCTGGC 
GTCCACACGG TGCCTGGGAG CCCTCAGGCG CGCCACAGAA CAGTGGGCAC CAACACTCCC 
CCTAGTCCTG GCTTCGGCTG GCGGGCCATC AATCCCAGCA TGGCTGCCCC CAGCAGTCCC 
AGTTTGAGCC ATCACCAGAT GATGGGTCCA CCAGGCACTG GCTTCCATGG TAGCACTGTC 
TCCAGCCCCC AGAGCAGTGC AGCGACCACC CCGGGGAGCC CCAGCCTGTG TCGGCACCCA 
GCAGGGGTCT ACCAGGTTTC TGGCCTCCAC AACAAAGTGG CCACCACCCC GGGGAGTCCC 
AGCCTGGGCC GGCACCCTGG GGCTCACCAA GGCAACCTGG CCTCCGGTCT TCATAGCAAT 
GCAATAGCCA GCCCTGGAAG CCCCAGCCTG GGCCGTCACC TCGGAGGGTC TGGATCTGTG 
GTTCCCGGCA GCCCCTGCTT GGACCGGCAT GTGGCCTATG GTGGCTATTC TACCCCGGAG 
GATCGGAGAC CCACACTGTC CCGGCAGAGC AGTGCCTCTG GCTACCAGGC TCCTTCCACG 
CCCTCCTTCC CTGTCTCCCC TGCCTACTAC CCTGGCCTGA GCAGCCCTGC CACCTCCCCG 
TCACCAGACT CCGCAGCCTT CCGGCAAGGG AGCCCAACAC CAGCCTTGCC AGAGAAGCGA 
AGGATGTCAG TGGGAGACCG GGCAGGCAGC CTCCCCAACT ATGCCACCAT CAATGGGAAG 
GTGTCTTCGC CTGTCGCCAG CGGCATGTCC AGTCCCAGTG GGGGCAGCAC CGTCTCCTTC 
TCCCACACTC TGCCCGACTT CTCCAAGTAC TCCATGCCAG ACAACAGCCC GGAGACGCGG 
GCTAAAGTGA AGTTTGTCCA GGACACTTCT AAGTATTGGT ACAAGCCTGA GATCTCCAGG 
GAGCAGGCCA TCGCGCTCCT CAAGGACCAG GAGCCGGGGG CCTTCATCAT CCGCGACAGT 
CACTCCTTCC GAGGCGCGTA CGGGCTGGCC ATGAAGGTGT CTTCGCCACC TCCAACCATC 
ATGCAGCAGA ATAAAAAAGG AGACATGACC CATGAGCTGG TCAGGCATTT TCTGATAGAG 
ACTGGCCCCA GAGGAGTCAA GCTCAAGGGC TGCCCCAATG AGCCAAACTT CGGATCGCTG 
TCTGCCCTGG TCTACCAGCA CTCCATCATC CCATTGGCCC TGCCTTGCAA GCTGGTCATT 
CCAAACCGAG ACCCCACAGA TGAATCGAAA GATAGCTCCG GCCCTGCCAA CTCAACTGCA 
GACCTGCTGA AACAAGGGGC AGCCTGCAAT GTGCTCTTCG TCAACTCTGT GGACATGGAG 
TCACTCACTG GGCCACAGGC CATCTCTAAA GCCACATCTG AGACGTTGGC TGCAGACCCC 
ACGCCAGCTG CCACCATCGT TCACTTCAAA GTCTCTGCCC AGGGAATCAC TCTGACTGAC 
AACCAGAGAA AGCTCTTTTT CAGACGCCAC TACCCTCTCA ACACTGTCAC CTTCTGTGAC 
CTGGATCCAC AGGAAAGAAA GTGGATGAAA ACAGAGGGTG GTGCCCCTGC TAAGCTCTTC 
GGCTTCGTGG CCCGGAAGCA GGGCAGCACC ACGGACAACG CCTGCCACCT CTTTGCTGAG 
CTTGACCCCA ACCAGCCGGC CTCTGCCATC GTCAACTTCG TCTCCAAGGT CATGCTGAAT 
GCCGGCCAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hic-5 regulates fibrillar adhesion formation to control tumor extracellular matrix remodeling through interaction with tensin1.
Oncogene(2018 Jan)
Goreczny GJ,Forsythe IJ,Turner CE

Tensin 1 Is Essential for Myofibroblast Differentiation and Extracellular Matrix Formation.
American journal of respiratory cell and molecular biology56(4)465-476(2017 Apr)
Bernau K,Torr EE,Evans MD,Aoki JK,Ngam CR,Sandbo N

MicroRNA-548j functions as a metastasis promoter in human breast cancer by targeting Tensin1.
Molecular oncology10(6)838-49(2016 06)
Zhan Y,Liang X,Li L,Wang B,Ding F,Li Y,Wang X,Zhan Q,Liu Z

Identification of Tyrosine-Phosphorylated Proteins Upregulated during Epithelial-Mesenchymal Transition Induced with TGF-?.
Journal of proteome research14(10)4127-36(2015 Oct)
Okayama A,Miyagi Y,Oshita F,Ito H,Nakayama H,Nishi M,Kurata Y,Kimura Y,Ryo A,Hirano H

Meta-analysis of genome-wide association studies of adult height in East Asians identifies 17 novel loci.
Human molecular genetics24(6)1791-800(2015 Mar)
He M,Xu M,Zhang B,Liang J,Chen P,Lee JY,Johnson TA,Li H,Yang X,Dai J,Liang L,Gui L,Qi Q,Huang J,Li Y,Adair LS,Aung T,Cai Q,Cheng CY,Cho MC,Cho YS,Chu M,Cui B,Gao YT,Go MJ,Gu D,Gu W,Guo H,Hao Y,Hong J,Hu Z,Hu Y,Huang J,Hwang JY,Ikram MK,Jin G,Kang DH,Khor CC,Kim BJ,Kim HT,Kubo M,Lee J,Lee J,Lee NR,Li R,Li J,Liu J,Longe J,Lu W,Lu X,Miao X,Okada Y,Ong RT,Qiu G,Seielstad M,Sim X,Song H,Takeuchi F,Tanaka T,Taylor PR,Wang L,Wang W,Wang Y,Wu C,Wu Y,Xiang YB,Yamamoto K,Yang H,Liao M,Yokota M,Young T,Zhang X,Kato N,Wang QK,Zheng W,Hu FB,Lin D,Shen H,Teo YY,Mo Z,Wong TY,Lin X,Mohlke KL,Ning G,Tsunoda T,Han BG,Shu XO,Tai ES,Wu T,Qi L

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TNS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: