TNS1 cDNA ORF clone, Bos taurus(cattle)

The following TNS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa227168 XM_024999078.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227169 XM_024999082.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227170 XM_024999090.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227170 XM_024999146.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227171 XM_024999100.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227172 XM_024999101.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227173 XM_024999105.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227174 XM_024999109.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227174 XM_024999158.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227175 XM_024999116.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227176 XM_024999120.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227177 XM_024999124.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227178 XM_024999129.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa227179 XM_024999135.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa227180 XM_024999138.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa227181 XM_024999142.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227182 XM_024999153.1
Latest version!
Bos taurus tensin 1 (TNS1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa41509 NM_174766.2
Latest version!
Bos taurus tensin 1 (TNS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa227168
    Clone ID Related Accession (Same CDS sequence) XM_024999078.1
    Accession Version XM_024999078.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GCGTACAATG CCAGGCTGCA GGGCACTGGG CGGAGAGTCA GCTCCTGGCA CCCTCCTCTC
    CACCGGCTCC GATCTTCCTC TCTCTCTGGG GTGCAGGCTC GGGAGAAGCA GCCTGCAGAG
    CCCCCAGCAC CTCTCCGGAA GCGGGCTGCC AGTGATGGGC AGTATGAGAA CCAGTCACCA
    GAACCCACAT CCCCCCGTAG CCCTGGGGTT CGCTCGCCTG TCCAATGTGT CTCCCCAGAG
    CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGACCCA AAGAGCCCCA TCTGCACAGC
    TACAAGGAAG CCTTTGAGGA GATGGAGGGA ACCTCTCCGA CCAGCCCACC ACCCAGTGGG
    GTGCGTTCCC CTCCAGGTCT GGCCAAGACA CCCTTGTCTG CTCTGGGCCT GAAGCCCCAC
    AACCCAGCCG ACATCCTGCT GCATCCCACT GGTGAGGAGG ATGAGAGGAA GGTGCTGACA
    GAGCTTTCTG AAGAGCCCCG GAGCTACGTG GAGTCGGTGG TACGAACGGC GGTGGCTGGG
    CCCCGGACTC AGGAACCTGA GCCCAAGAGC TTTAGCGCCC CAGGTGCGCA GGCGTATGGC
    CATGAGACAC CCCTGAGGAT TGGGACCCTG GGAGGCTCCT TTGTCTCCCC TAGCCCCCTC
    TCCACCAGCA GCCCCATCCT CAGTGCTGAC AGCACTTCAG TGGGGAGTTT CCCATCCGGG
    GAGAGCAGCG ACCAGGGCGC GCGAACACCT ACCCAGCCTC TGCTGGATTC TGGCTTCCGC
    TCCGGCAGCC TGGGCCAGCC AAGTCCTTTG GCTCAGAGAA ACTACCAGAG CTCTTCTCCT
    CTCCCAACTG CAGGCAGCAG CTACAGCAGC CCCGACTACT CACTGCAGCA GTTCAGCTCC
    CCAGAAGGCC AGGCCCGGTC TCAGTTCAGT GTGGCCGGTG TCCACACAGT GCCTGGGAGC
    CCCCAGGCAC GGCACAGGAC AGTGGGCACC AATACACCCC CGAGCCCTGG CTTTGGCCGT
    CGGGCTGTGA ACCCCAGCCT GGCTGCCCCC AGCAGCCCCA GTTTGAGCCA CCGCCAGGTG
    ATGGGTCCAC TGGGAACCGG TTTCCATGGT AACACGGGCT CCAGCCCCCA GAGCAGTGCA
    GCAACCACTC CGGGAAGCCC CAGTCTGGGT CGCCACCCTG GGGCCCACCA GGTTTCCAAC
    CTCCATGGCA ATGTGGTCAC CGCTCCTGGG AGCCCCAGCC TGGGCCGGCA CCCCGGGGCC
    CACCAAGGCA CCCTGGCCTC CAATCTCCAT AGCAATGCAG TAGCCAGCCC CGGAAGCCCC
    AGCCTGGGCC GTCACTTGGG AGGGTCTGGA TCTGTGGTTC CCGGCAGCCC CAGCTTGGAC
    CGGCACGTGC CCTATGGTGG CTACTCCACC CCTGAGGACC GGAGACCCAC GCTGTCCCGG
    CAGAGCAGCG CCTCAGGCTA CCAGGCTCCT TCCACGCCCT CCTTCCCTGT GTCCCCCGCC
    TACTACCCTG GCCTGAGCAG CCCCGCCACC TCCCCGTCGC CTGATTCGGC AGCTTTCCGA
    CAAGGGAGCC CCACCCCGGC CTTGCCGGAG AAGCGGAGGA TGTCCATGGG CGACCGAGCG
    GGCAGCCTCC CCAACTACGC CACCGTCAAC GGGAAGGTGT CCTCCTCGCC GGTCGCCAGT
    GGCATGTCCA GTCCCAGCGG GGGCAGCACC GTCTCCTTCT CCCACACTCT GCCCGACTTC
    TCCAAGTACT CCATGCCAGA CAACAGTCCT GAGACGCGGG CTAAAGTGAA GTTTGTCCAA
    GACACTTCCA AGTATTGGTA CAAGCCTGAG ATCTCCAGAG AACAGGCCAT CGCCCTCCTT
    AAGGACCAGG AGCCAGGGGC TTTCATCATC CGTGACAGCC ACTCCTTCCG AGGAGCCTAT
    GGGCTGGCCA TGAAGGTGTC TTCGCCCCCT CCGACCATCA TGCAGCAGAA TAAAAAAGGG
    GACATGACCC ACGAGCTCGT GAGGCATTTC CTGATAGAGA CCGGCCCCAG AGGAGTCAAG
    CTCAAGGGCT GCCCCAATGA GCCAAACTTC GGATCTCTGT CTGCCCTGGT CTACCAGCAC
    TCCATCATCC CGCTGGCCCT GCCCTGCAAG CTGGTCATTC CAAACCGAGA CCCCACAGAT
    GAATCGAAAG ATAGCTCGGG CCCTGCCAAC TCAACCAGTG ACCTGCTGAA GCAAGGGGCA
    GCCTGCAACG TGCTCTTCGT CAACTCTGTG GACATGGAGT CACTCACTGG GCCACAGGCC
    ATCTCCAAAG CCATTTCTGA GACCCTGGCT GCTGACCCCA CACCAGCTGC CACCATCGTT
    CACTTCAAAG TTTCTGCCCA GGGAATTACA CTGACTGACA ACCAGAGAAA GCTTTTCTTC
    AGGCGGCACT ACCCCCTCAA CACTGTGACC TTCTGTGACC TGGATCCACA GGAGAGGAAG
    TGGACAAAGA CGGAGGGAGG TGCCCCTGCT AAGCTCTTCG GTTTTGTGGC CCGGAAACAG
    GGCAGCGCCA CGGACAACGC CTGCCACCTC TTTGCTGAGC TTGACCCCAA CCAGCCGGCC
    TCCGCCATCG TCAGCTTTGT CTCCAAGGTC ATGCTGAGTG CCGGCCAGAA GAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854846.1
    CDS343..5619
    Translation

    Target ORF information:

    RefSeq Version XM_024999078.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999078.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GCGTACAATG CCAGGCTGCA GGGCACTGGG CGGAGAGTCA GCTCCTGGCA CCCTCCTCTC
    CACCGGCTCC GATCTTCCTC TCTCTCTGGG GTGCAGGCTC GGGAGAAGCA GCCTGCAGAG
    CCCCCAGCAC CTCTCCGGAA GCGGGCTGCC AGTGATGGGC AGTATGAGAA CCAGTCACCA
    GAACCCACAT CCCCCCGTAG CCCTGGGGTT CGCTCGCCTG TCCAATGTGT CTCCCCAGAG
    CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGACCCA AAGAGCCCCA TCTGCACAGC
    TACAAGGAAG CCTTTGAGGA GATGGAGGGA ACCTCTCCGA CCAGCCCACC ACCCAGTGGG
    GTGCGTTCCC CTCCAGGTCT GGCCAAGACA CCCTTGTCTG CTCTGGGCCT GAAGCCCCAC
    AACCCAGCCG ACATCCTGCT GCATCCCACT GGTGAGGAGG ATGAGAGGAA GGTGCTGACA
    GAGCTTTCTG AAGAGCCCCG GAGCTACGTG GAGTCGGTGG TACGAACGGC GGTGGCTGGG
    CCCCGGACTC AGGAACCTGA GCCCAAGAGC TTTAGCGCCC CAGGTGCGCA GGCGTATGGC
    CATGAGACAC CCCTGAGGAT TGGGACCCTG GGAGGCTCCT TTGTCTCCCC TAGCCCCCTC
    TCCACCAGCA GCCCCATCCT CAGTGCTGAC AGCACTTCAG TGGGGAGTTT CCCATCCGGG
    GAGAGCAGCG ACCAGGGCGC GCGAACACCT ACCCAGCCTC TGCTGGATTC TGGCTTCCGC
    TCCGGCAGCC TGGGCCAGCC AAGTCCTTTG GCTCAGAGAA ACTACCAGAG CTCTTCTCCT
    CTCCCAACTG CAGGCAGCAG CTACAGCAGC CCCGACTACT CACTGCAGCA GTTCAGCTCC
    CCAGAAGGCC AGGCCCGGTC TCAGTTCAGT GTGGCCGGTG TCCACACAGT GCCTGGGAGC
    CCCCAGGCAC GGCACAGGAC AGTGGGCACC AATACACCCC CGAGCCCTGG CTTTGGCCGT
    CGGGCTGTGA ACCCCAGCCT GGCTGCCCCC AGCAGCCCCA GTTTGAGCCA CCGCCAGGTG
    ATGGGTCCAC TGGGAACCGG TTTCCATGGT AACACGGGCT CCAGCCCCCA GAGCAGTGCA
    GCAACCACTC CGGGAAGCCC CAGTCTGGGT CGCCACCCTG GGGCCCACCA GGTTTCCAAC
    CTCCATGGCA ATGTGGTCAC CGCTCCTGGG AGCCCCAGCC TGGGCCGGCA CCCCGGGGCC
    CACCAAGGCA CCCTGGCCTC CAATCTCCAT AGCAATGCAG TAGCCAGCCC CGGAAGCCCC
    AGCCTGGGCC GTCACTTGGG AGGGTCTGGA TCTGTGGTTC CCGGCAGCCC CAGCTTGGAC
    CGGCACGTGC CCTATGGTGG CTACTCCACC CCTGAGGACC GGAGACCCAC GCTGTCCCGG
    CAGAGCAGCG CCTCAGGCTA CCAGGCTCCT TCCACGCCCT CCTTCCCTGT GTCCCCCGCC
    TACTACCCTG GCCTGAGCAG CCCCGCCACC TCCCCGTCGC CTGATTCGGC AGCTTTCCGA
    CAAGGGAGCC CCACCCCGGC CTTGCCGGAG AAGCGGAGGA TGTCCATGGG CGACCGAGCG
    GGCAGCCTCC CCAACTACGC CACCGTCAAC GGGAAGGTGT CCTCCTCGCC GGTCGCCAGT
    GGCATGTCCA GTCCCAGCGG GGGCAGCACC GTCTCCTTCT CCCACACTCT GCCCGACTTC
    TCCAAGTACT CCATGCCAGA CAACAGTCCT GAGACGCGGG CTAAAGTGAA GTTTGTCCAA
    GACACTTCCA AGTATTGGTA CAAGCCTGAG ATCTCCAGAG AACAGGCCAT CGCCCTCCTT
    AAGGACCAGG AGCCAGGGGC TTTCATCATC CGTGACAGCC ACTCCTTCCG AGGAGCCTAT
    GGGCTGGCCA TGAAGGTGTC TTCGCCCCCT CCGACCATCA TGCAGCAGAA TAAAAAAGGG
    GACATGACCC ACGAGCTCGT GAGGCATTTC CTGATAGAGA CCGGCCCCAG AGGAGTCAAG
    CTCAAGGGCT GCCCCAATGA GCCAAACTTC GGATCTCTGT CTGCCCTGGT CTACCAGCAC
    TCCATCATCC CGCTGGCCCT GCCCTGCAAG CTGGTCATTC CAAACCGAGA CCCCACAGAT
    GAATCGAAAG ATAGCTCGGG CCCTGCCAAC TCAACCAGTG ACCTGCTGAA GCAAGGGGCA
    GCCTGCAACG TGCTCTTCGT CAACTCTGTG GACATGGAGT CACTCACTGG GCCACAGGCC
    ATCTCCAAAG CCATTTCTGA GACCCTGGCT GCTGACCCCA CACCAGCTGC CACCATCGTT
    CACTTCAAAG TTTCTGCCCA GGGAATTACA CTGACTGACA ACCAGAGAAA GCTTTTCTTC
    AGGCGGCACT ACCCCCTCAA CACTGTGACC TTCTGTGACC TGGATCCACA GGAGAGGAAG
    TGGACAAAGA CGGAGGGAGG TGCCCCTGCT AAGCTCTTCG GTTTTGTGGC CCGGAAACAG
    GGCAGCGCCA CGGACAACGC CTGCCACCTC TTTGCTGAGC TTGACCCCAA CCAGCCGGCC
    TCCGCCATCG TCAGCTTTGT CTCCAAGGTC ATGCTGAGTG CCGGCCAGAA GAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227169
    Clone ID Related Accession (Same CDS sequence) XM_024999082.1
    Accession Version XM_024999082.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GCGTACAATG CCAGGCTGCA GGGCACTGGG CGGAGAGTCA GCTCCTGGCA CCCTCCTCTC
    CACCGGCTCC GATCTTCCTC TCTCTCTGGG GTGCAGGCTC GGGAGAAGCA GCCTGCAGAG
    CCCCCAGCAC CTCTCCGGAA GCGGGCTGCC AGTGATGGGC AGTATGAGAA CCAGTCACCA
    GAACCCACAT CCCCCCGTAG CCCTGGGGTT CGCTCGCCTG TCCAATGTGT CTCCCCAGAG
    CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGACCCA AAGAGCCCCA TCTGCACAGC
    TACAAGGAAG CCTTTGAGGA GATGGAGGGA ACCTCTCCGA CCAGCCCACC ACCCAGTGGG
    GTGCGTTCCC CTCCAGGTCT GGCCAAGACA CCCTTGTCTG CTCTGGGCCT GAAGCCCCAC
    AACCCAGCCG ACATCCTGCT GCATCCCACT GGTGTCCCCA GAAGACTGAT CCAGCCAGAG
    CCCCGGAGCT ACGTGGAGTC GGTGGTACGA ACGGCGGTGG CTGGGCCCCG GACTCAGGAA
    CCTGAGCCCA AGAGCTTTAG CGCCCCAGGT GCGCAGGCGT ATGGCCATGA GACACCCCTG
    AGGATTGGGA CCCTGGGAGG CTCCTTTGTC TCCCCTAGCC CCCTCTCCAC CAGCAGCCCC
    ATCCTCAGTG CTGACAGCAC TTCAGTGGGG AGTTTCCCAT CCGGGGAGAG CAGCGACCAG
    GGCGCGCGAA CACCTACCCA GCCTCTGCTG GATTCTGGCT TCCGCTCCGG CAGCCTGGGC
    CAGCCAAGTC CTTTGGCTCA GAGAAACTAC CAGAGCTCTT CTCCTCTCCC AACTGCAGGC
    AGCAGCTACA GCAGCCCCGA CTACTCACTG CAGCAGTTCA GCTCCCCAGA AGGCCAGGCC
    CGGTCTCAGT TCAGTGTGGC CGGTGTCCAC ACAGTGCCTG GGAGCCCCCA GGCACGGCAC
    AGGACAGTGG GCACCAATAC ACCCCCGAGC CCTGGCTTTG GCCGTCGGGC TGTGAACCCC
    AGCCTGGCTG CCCCCAGCAG CCCCAGTTTG AGCCACCGCC AGGTGATGGG TCCACTGGGA
    ACCGGTTTCC ATGGTAACAC GGGCTCCAGC CCCCAGAGCA GTGCAGCAAC CACTCCGGGA
    AGCCCCAGTC TGGGTCGCCA CCCTGGGGCC CACCAGGTTT CCAACCTCCA TGGCAATGTG
    GTCACCGCTC CTGGGAGCCC CAGCCTGGGC CGGCACCCCG GGGCCCACCA AGGCACCCTG
    GCCTCCAATC TCCATAGCAA TGCAGTAGCC AGCCCCGGAA GCCCCAGCCT GGGCCGTCAC
    TTGGGAGGGT CTGGATCTGT GGTTCCCGGC AGCCCCAGCT TGGACCGGCA CGTGCCCTAT
    GGTGGCTACT CCACCCCTGA GGACCGGAGA CCCACGCTGT CCCGGCAGAG CAGCGCCTCA
    GGCTACCAGG CTCCTTCCAC GCCCTCCTTC CCTGTGTCCC CCGCCTACTA CCCTGGCCTG
    AGCAGCCCCG CCACCTCCCC GTCGCCTGAT TCGGCAGCTT TCCGACAAGG GAGCCCCACC
    CCGGCCTTGC CGGAGAAGCG GAGGATGTCC ATGGGCGACC GAGCGGGCAG CCTCCCCAAC
    TACGCCACCG TCAACGGGAA GGTGTCCTCC TCGCCGGTCG CCAGTGGCAT GTCCAGTCCC
    AGCGGGGGCA GCACCGTCTC CTTCTCCCAC ACTCTGCCCG ACTTCTCCAA GTACTCCATG
    CCAGACAACA GTCCTGAGAC GCGGGCTAAA GTGAAGTTTG TCCAAGACAC TTCCAAGTAT
    TGGTACAAGC CTGAGATCTC CAGAGAACAG GCCATCGCCC TCCTTAAGGA CCAGGAGCCA
    GGGGCTTTCA TCATCCGTGA CAGCCACTCC TTCCGAGGAG CCTATGGGCT GGCCATGAAG
    GTGTCTTCGC CCCCTCCGAC CATCATGCAG CAGAATAAAA AAGGGGACAT GACCCACGAG
    CTCGTGAGGC ATTTCCTGAT AGAGACCGGC CCCAGAGGAG TCAAGCTCAA GGGCTGCCCC
    AATGAGCCAA ACTTCGGATC TCTGTCTGCC CTGGTCTACC AGCACTCCAT CATCCCGCTG
    GCCCTGCCCT GCAAGCTGGT CATTCCAAAC CGAGACCCCA CAGATGAATC GAAAGATAGC
    TCGGGCCCTG CCAACTCAAC CAGTGACCTG CTGAAGCAAG GGGCAGCCTG CAACGTGCTC
    TTCGTCAACT CTGTGGACAT GGAGTCACTC ACTGGGCCAC AGGCCATCTC CAAAGCCATT
    TCTGAGACCC TGGCTGCTGA CCCCACACCA GCTGCCACCA TCGTTCACTT CAAAGTTTCT
    GCCCAGGGAA TTACACTGAC TGACAACCAG AGAAAGCTTT TCTTCAGGCG GCACTACCCC
    CTCAACACTG TGACCTTCTG TGACCTGGAT CCACAGGAGA GGAAGTGGAC AAAGACGGAG
    GGAGGTGCCC CTGCTAAGCT CTTCGGTTTT GTGGCCCGGA AACAGGGCAG CGCCACGGAC
    AACGCCTGCC ACCTCTTTGC TGAGCTTGAC CCCAACCAGC CGGCCTCCGC CATCGTCAGC
    TTTGTCTCCA AGGTCATGCT GAGTGCCGGC CAGAAGAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854850.1
    CDS343..5604
    Translation

    Target ORF information:

    RefSeq Version XM_024999082.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999082.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GCGTACAATG CCAGGCTGCA GGGCACTGGG CGGAGAGTCA GCTCCTGGCA CCCTCCTCTC
    CACCGGCTCC GATCTTCCTC TCTCTCTGGG GTGCAGGCTC GGGAGAAGCA GCCTGCAGAG
    CCCCCAGCAC CTCTCCGGAA GCGGGCTGCC AGTGATGGGC AGTATGAGAA CCAGTCACCA
    GAACCCACAT CCCCCCGTAG CCCTGGGGTT CGCTCGCCTG TCCAATGTGT CTCCCCAGAG
    CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGACCCA AAGAGCCCCA TCTGCACAGC
    TACAAGGAAG CCTTTGAGGA GATGGAGGGA ACCTCTCCGA CCAGCCCACC ACCCAGTGGG
    GTGCGTTCCC CTCCAGGTCT GGCCAAGACA CCCTTGTCTG CTCTGGGCCT GAAGCCCCAC
    AACCCAGCCG ACATCCTGCT GCATCCCACT GGTGTCCCCA GAAGACTGAT CCAGCCAGAG
    CCCCGGAGCT ACGTGGAGTC GGTGGTACGA ACGGCGGTGG CTGGGCCCCG GACTCAGGAA
    CCTGAGCCCA AGAGCTTTAG CGCCCCAGGT GCGCAGGCGT ATGGCCATGA GACACCCCTG
    AGGATTGGGA CCCTGGGAGG CTCCTTTGTC TCCCCTAGCC CCCTCTCCAC CAGCAGCCCC
    ATCCTCAGTG CTGACAGCAC TTCAGTGGGG AGTTTCCCAT CCGGGGAGAG CAGCGACCAG
    GGCGCGCGAA CACCTACCCA GCCTCTGCTG GATTCTGGCT TCCGCTCCGG CAGCCTGGGC
    CAGCCAAGTC CTTTGGCTCA GAGAAACTAC CAGAGCTCTT CTCCTCTCCC AACTGCAGGC
    AGCAGCTACA GCAGCCCCGA CTACTCACTG CAGCAGTTCA GCTCCCCAGA AGGCCAGGCC
    CGGTCTCAGT TCAGTGTGGC CGGTGTCCAC ACAGTGCCTG GGAGCCCCCA GGCACGGCAC
    AGGACAGTGG GCACCAATAC ACCCCCGAGC CCTGGCTTTG GCCGTCGGGC TGTGAACCCC
    AGCCTGGCTG CCCCCAGCAG CCCCAGTTTG AGCCACCGCC AGGTGATGGG TCCACTGGGA
    ACCGGTTTCC ATGGTAACAC GGGCTCCAGC CCCCAGAGCA GTGCAGCAAC CACTCCGGGA
    AGCCCCAGTC TGGGTCGCCA CCCTGGGGCC CACCAGGTTT CCAACCTCCA TGGCAATGTG
    GTCACCGCTC CTGGGAGCCC CAGCCTGGGC CGGCACCCCG GGGCCCACCA AGGCACCCTG
    GCCTCCAATC TCCATAGCAA TGCAGTAGCC AGCCCCGGAA GCCCCAGCCT GGGCCGTCAC
    TTGGGAGGGT CTGGATCTGT GGTTCCCGGC AGCCCCAGCT TGGACCGGCA CGTGCCCTAT
    GGTGGCTACT CCACCCCTGA GGACCGGAGA CCCACGCTGT CCCGGCAGAG CAGCGCCTCA
    GGCTACCAGG CTCCTTCCAC GCCCTCCTTC CCTGTGTCCC CCGCCTACTA CCCTGGCCTG
    AGCAGCCCCG CCACCTCCCC GTCGCCTGAT TCGGCAGCTT TCCGACAAGG GAGCCCCACC
    CCGGCCTTGC CGGAGAAGCG GAGGATGTCC ATGGGCGACC GAGCGGGCAG CCTCCCCAAC
    TACGCCACCG TCAACGGGAA GGTGTCCTCC TCGCCGGTCG CCAGTGGCAT GTCCAGTCCC
    AGCGGGGGCA GCACCGTCTC CTTCTCCCAC ACTCTGCCCG ACTTCTCCAA GTACTCCATG
    CCAGACAACA GTCCTGAGAC GCGGGCTAAA GTGAAGTTTG TCCAAGACAC TTCCAAGTAT
    TGGTACAAGC CTGAGATCTC CAGAGAACAG GCCATCGCCC TCCTTAAGGA CCAGGAGCCA
    GGGGCTTTCA TCATCCGTGA CAGCCACTCC TTCCGAGGAG CCTATGGGCT GGCCATGAAG
    GTGTCTTCGC CCCCTCCGAC CATCATGCAG CAGAATAAAA AAGGGGACAT GACCCACGAG
    CTCGTGAGGC ATTTCCTGAT AGAGACCGGC CCCAGAGGAG TCAAGCTCAA GGGCTGCCCC
    AATGAGCCAA ACTTCGGATC TCTGTCTGCC CTGGTCTACC AGCACTCCAT CATCCCGCTG
    GCCCTGCCCT GCAAGCTGGT CATTCCAAAC CGAGACCCCA CAGATGAATC GAAAGATAGC
    TCGGGCCCTG CCAACTCAAC CAGTGACCTG CTGAAGCAAG GGGCAGCCTG CAACGTGCTC
    TTCGTCAACT CTGTGGACAT GGAGTCACTC ACTGGGCCAC AGGCCATCTC CAAAGCCATT
    TCTGAGACCC TGGCTGCTGA CCCCACACCA GCTGCCACCA TCGTTCACTT CAAAGTTTCT
    GCCCAGGGAA TTACACTGAC TGACAACCAG AGAAAGCTTT TCTTCAGGCG GCACTACCCC
    CTCAACACTG TGACCTTCTG TGACCTGGAT CCACAGGAGA GGAAGTGGAC AAAGACGGAG
    GGAGGTGCCC CTGCTAAGCT CTTCGGTTTT GTGGCCCGGA AACAGGGCAG CGCCACGGAC
    AACGCCTGCC ACCTCTTTGC TGAGCTTGAC CCCAACCAGC CGGCCTCCGC CATCGTCAGC
    TTTGTCTCCA AGGTCATGCT GAGTGCCGGC CAGAAGAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227170
    Clone ID Related Accession (Same CDS sequence) XM_024999146.1 , XM_024999090.1
    Accession Version XM_024999146.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GCGTACAATG CCAGGCTGCA GGGCACTGGG CGGAGAGTCA GCTCCTGGCA CCCTCCTCTC
    CACCGGCTCC GATCTTCCTC TCTCTCTGGG GTGCAGGCTC GGGAGAAGCA GCCTGCAGAG
    CCCCCAGCAC CTCTCCGGAA GCGGGCTGCC AGTGATGGGC AGTATGAGAA CCAGTCACCA
    GAACCCACAT CCCCCCGTAG CCCTGGGGTT CGCTCGCCTG TCCAATGTGT CTCCCCAGAG
    CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGACCCA AAGAGCCCCA TCTGCACAGC
    TACAAGGAAG CCTTTGAGGA GATGGAGGGA ACCTCTCCGA CCAGCCCACC ACCCAGTGGG
    GTGCGTTCCC CTCCAGGTCT GGCCAAGACA CCCTTGTCTG CTCTGGGCCT GAAGCCCCAC
    AACCCAGCCG ACATCCTGCT GCATCCCACT GGTGAGCCCC GGAGCTACGT GGAGTCGGTG
    GTACGAACGG CGGTGGCTGG GCCCCGGACT CAGGAACCTG AGCCCAAGAG CTTTAGCGCC
    CCAGGTGCGC AGGCGTATGG CCATGAGACA CCCCTGAGGA TTGGGACCCT GGGAGGCTCC
    TTTGTCTCCC CTAGCCCCCT CTCCACCAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCA
    GTGGGGAGTT TCCCATCCGG GGAGAGCAGC GACCAGGGCG CGCGAACACC TACCCAGCCT
    CTGCTGGATT CTGGCTTCCG CTCCGGCAGC CTGGGCCAGC CAAGTCCTTT GGCTCAGAGA
    AACTACCAGA GCTCTTCTCC TCTCCCAACT GCAGGCAGCA GCTACAGCAG CCCCGACTAC
    TCACTGCAGC AGTTCAGCTC CCCAGAAGGC CAGGCCCGGT CTCAGTTCAG TGTGGCCGGT
    GTCCACACAG TGCCTGGGAG CCCCCAGGCA CGGCACAGGA CAGTGGGCAC CAATACACCC
    CCGAGCCCTG GCTTTGGCCG TCGGGCTGTG AACCCCAGCC TGGCTGCCCC CAGCAGCCCC
    AGTTTGAGCC ACCGCCAGGT GATGGGTCCA CTGGGAACCG GTTTCCATGG TAACACGGGC
    TCCAGCCCCC AGAGCAGTGC AGCAACCACT CCGGGAAGCC CCAGTCTGGG TCGCCACCCT
    GGGGCCCACC AGGTTTCCAA CCTCCATGGC AATGTGGTCA CCGCTCCTGG GAGCCCCAGC
    CTGGGCCGGC ACCCCGGGGC CCACCAAGGC ACCCTGGCCT CCAATCTCCA TAGCAATGCA
    GTAGCCAGCC CCGGAAGCCC CAGCCTGGGC CGTCACTTGG GAGGGTCTGG ATCTGTGGTT
    CCCGGCAGCC CCAGCTTGGA CCGGCACGTG CCCTATGGTG GCTACTCCAC CCCTGAGGAC
    CGGAGACCCA CGCTGTCCCG GCAGAGCAGC GCCTCAGGCT ACCAGGCTCC TTCCACGCCC
    TCCTTCCCTG TGTCCCCCGC CTACTACCCT GGCCTGAGCA GCCCCGCCAC CTCCCCGTCG
    CCTGATTCGG CAGCTTTCCG ACAAGGGAGC CCCACCCCGG CCTTGCCGGA GAAGCGGAGG
    ATGTCCATGG GCGACCGAGC GGGCAGCCTC CCCAACTACG CCACCGTCAA CGGGAAGGTG
    TCCTCCTCGC CGGTCGCCAG TGGCATGTCC AGTCCCAGCG GGGGCAGCAC CGTCTCCTTC
    TCCCACACTC TGCCCGACTT CTCCAAGTAC TCCATGCCAG ACAACAGTCC TGAGACGCGG
    GCTAAAGTGA AGTTTGTCCA AGACACTTCC AAGTATTGGT ACAAGCCTGA GATCTCCAGA
    GAACAGGCCA TCGCCCTCCT TAAGGACCAG GAGCCAGGGG CTTTCATCAT CCGTGACAGC
    CACTCCTTCC GAGGAGCCTA TGGGCTGGCC ATGAAGGTGT CTTCGCCCCC TCCGACCATC
    ATGCAGCAGA ATAAAAAAGG GGACATGACC CACGAGCTCG TGAGGCATTT CCTGATAGAG
    ACCGGCCCCA GAGGAGTCAA GCTCAAGGGC TGCCCCAATG AGCCAAACTT CGGATCTCTG
    TCTGCCCTGG TCTACCAGCA CTCCATCATC CCGCTGGCCC TGCCCTGCAA GCTGGTCATT
    CCAAACCGAG ACCCCACAGA TGAATCGAAA GATAGCTCGG GCCCTGCCAA CTCAACCAGT
    GACCTGCTGA AGCAAGGGGC AGCCTGCAAC GTGCTCTTCG TCAACTCTGT GGACATGGAG
    TCACTCACTG GGCCACAGGC CATCTCCAAA GCCATTTCTG AGACCCTGGC TGCTGACCCC
    ACACCAGCTG CCACCATCGT TCACTTCAAA GTTTCTGCCC AGGGAATTAC ACTGACTGAC
    AACCAGAGAA AGCTTTTCTT CAGGCGGCAC TACCCCCTCA ACACTGTGAC CTTCTGTGAC
    CTGGATCCAC AGGAGAGGAA GTGGACAAAG ACGGAGGGAG GTGCCCCTGC TAAGCTCTTC
    GGTTTTGTGG CCCGGAAACA GGGCAGCGCC ACGGACAACG CCTGCCACCT CTTTGCTGAG
    CTTGACCCCA ACCAGCCGGC CTCCGCCATC GTCAGCTTTG TCTCCAAGGT CATGCTGAGT
    GCCGGCCAGA AGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854914.1
    CDS412..5649
    Translation

    Target ORF information:

    RefSeq Version XM_024999146.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999146.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GCGTACAATG CCAGGCTGCA GGGCACTGGG CGGAGAGTCA GCTCCTGGCA CCCTCCTCTC
    CACCGGCTCC GATCTTCCTC TCTCTCTGGG GTGCAGGCTC GGGAGAAGCA GCCTGCAGAG
    CCCCCAGCAC CTCTCCGGAA GCGGGCTGCC AGTGATGGGC AGTATGAGAA CCAGTCACCA
    GAACCCACAT CCCCCCGTAG CCCTGGGGTT CGCTCGCCTG TCCAATGTGT CTCCCCAGAG
    CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGACCCA AAGAGCCCCA TCTGCACAGC
    TACAAGGAAG CCTTTGAGGA GATGGAGGGA ACCTCTCCGA CCAGCCCACC ACCCAGTGGG
    GTGCGTTCCC CTCCAGGTCT GGCCAAGACA CCCTTGTCTG CTCTGGGCCT GAAGCCCCAC
    AACCCAGCCG ACATCCTGCT GCATCCCACT GGTGAGCCCC GGAGCTACGT GGAGTCGGTG
    GTACGAACGG CGGTGGCTGG GCCCCGGACT CAGGAACCTG AGCCCAAGAG CTTTAGCGCC
    CCAGGTGCGC AGGCGTATGG CCATGAGACA CCCCTGAGGA TTGGGACCCT GGGAGGCTCC
    TTTGTCTCCC CTAGCCCCCT CTCCACCAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCA
    GTGGGGAGTT TCCCATCCGG GGAGAGCAGC GACCAGGGCG CGCGAACACC TACCCAGCCT
    CTGCTGGATT CTGGCTTCCG CTCCGGCAGC CTGGGCCAGC CAAGTCCTTT GGCTCAGAGA
    AACTACCAGA GCTCTTCTCC TCTCCCAACT GCAGGCAGCA GCTACAGCAG CCCCGACTAC
    TCACTGCAGC AGTTCAGCTC CCCAGAAGGC CAGGCCCGGT CTCAGTTCAG TGTGGCCGGT
    GTCCACACAG TGCCTGGGAG CCCCCAGGCA CGGCACAGGA CAGTGGGCAC CAATACACCC
    CCGAGCCCTG GCTTTGGCCG TCGGGCTGTG AACCCCAGCC TGGCTGCCCC CAGCAGCCCC
    AGTTTGAGCC ACCGCCAGGT GATGGGTCCA CTGGGAACCG GTTTCCATGG TAACACGGGC
    TCCAGCCCCC AGAGCAGTGC AGCAACCACT CCGGGAAGCC CCAGTCTGGG TCGCCACCCT
    GGGGCCCACC AGGTTTCCAA CCTCCATGGC AATGTGGTCA CCGCTCCTGG GAGCCCCAGC
    CTGGGCCGGC ACCCCGGGGC CCACCAAGGC ACCCTGGCCT CCAATCTCCA TAGCAATGCA
    GTAGCCAGCC CCGGAAGCCC CAGCCTGGGC CGTCACTTGG GAGGGTCTGG ATCTGTGGTT
    CCCGGCAGCC CCAGCTTGGA CCGGCACGTG CCCTATGGTG GCTACTCCAC CCCTGAGGAC
    CGGAGACCCA CGCTGTCCCG GCAGAGCAGC GCCTCAGGCT ACCAGGCTCC TTCCACGCCC
    TCCTTCCCTG TGTCCCCCGC CTACTACCCT GGCCTGAGCA GCCCCGCCAC CTCCCCGTCG
    CCTGATTCGG CAGCTTTCCG ACAAGGGAGC CCCACCCCGG CCTTGCCGGA GAAGCGGAGG
    ATGTCCATGG GCGACCGAGC GGGCAGCCTC CCCAACTACG CCACCGTCAA CGGGAAGGTG
    TCCTCCTCGC CGGTCGCCAG TGGCATGTCC AGTCCCAGCG GGGGCAGCAC CGTCTCCTTC
    TCCCACACTC TGCCCGACTT CTCCAAGTAC TCCATGCCAG ACAACAGTCC TGAGACGCGG
    GCTAAAGTGA AGTTTGTCCA AGACACTTCC AAGTATTGGT ACAAGCCTGA GATCTCCAGA
    GAACAGGCCA TCGCCCTCCT TAAGGACCAG GAGCCAGGGG CTTTCATCAT CCGTGACAGC
    CACTCCTTCC GAGGAGCCTA TGGGCTGGCC ATGAAGGTGT CTTCGCCCCC TCCGACCATC
    ATGCAGCAGA ATAAAAAAGG GGACATGACC CACGAGCTCG TGAGGCATTT CCTGATAGAG
    ACCGGCCCCA GAGGAGTCAA GCTCAAGGGC TGCCCCAATG AGCCAAACTT CGGATCTCTG
    TCTGCCCTGG TCTACCAGCA CTCCATCATC CCGCTGGCCC TGCCCTGCAA GCTGGTCATT
    CCAAACCGAG ACCCCACAGA TGAATCGAAA GATAGCTCGG GCCCTGCCAA CTCAACCAGT
    GACCTGCTGA AGCAAGGGGC AGCCTGCAAC GTGCTCTTCG TCAACTCTGT GGACATGGAG
    TCACTCACTG GGCCACAGGC CATCTCCAAA GCCATTTCTG AGACCCTGGC TGCTGACCCC
    ACACCAGCTG CCACCATCGT TCACTTCAAA GTTTCTGCCC AGGGAATTAC ACTGACTGAC
    AACCAGAGAA AGCTTTTCTT CAGGCGGCAC TACCCCCTCA ACACTGTGAC CTTCTGTGAC
    CTGGATCCAC AGGAGAGGAA GTGGACAAAG ACGGAGGGAG GTGCCCCTGC TAAGCTCTTC
    GGTTTTGTGG CCCGGAAACA GGGCAGCGCC ACGGACAACG CCTGCCACCT CTTTGCTGAG
    CTTGACCCCA ACCAGCCGGC CTCCGCCATC GTCAGCTTTG TCTCCAAGGT CATGCTGAGT
    GCCGGCCAGA AGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227170
    Clone ID Related Accession (Same CDS sequence) XM_024999146.1 , XM_024999090.1
    Accession Version XM_024999090.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5238bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GCGTACAATG CCAGGCTGCA GGGCACTGGG CGGAGAGTCA GCTCCTGGCA CCCTCCTCTC
    CACCGGCTCC GATCTTCCTC TCTCTCTGGG GTGCAGGCTC GGGAGAAGCA GCCTGCAGAG
    CCCCCAGCAC CTCTCCGGAA GCGGGCTGCC AGTGATGGGC AGTATGAGAA CCAGTCACCA
    GAACCCACAT CCCCCCGTAG CCCTGGGGTT CGCTCGCCTG TCCAATGTGT CTCCCCAGAG
    CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGACCCA AAGAGCCCCA TCTGCACAGC
    TACAAGGAAG CCTTTGAGGA GATGGAGGGA ACCTCTCCGA CCAGCCCACC ACCCAGTGGG
    GTGCGTTCCC CTCCAGGTCT GGCCAAGACA CCCTTGTCTG CTCTGGGCCT GAAGCCCCAC
    AACCCAGCCG ACATCCTGCT GCATCCCACT GGTGAGCCCC GGAGCTACGT GGAGTCGGTG
    GTACGAACGG CGGTGGCTGG GCCCCGGACT CAGGAACCTG AGCCCAAGAG CTTTAGCGCC
    CCAGGTGCGC AGGCGTATGG CCATGAGACA CCCCTGAGGA TTGGGACCCT GGGAGGCTCC
    TTTGTCTCCC CTAGCCCCCT CTCCACCAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCA
    GTGGGGAGTT TCCCATCCGG GGAGAGCAGC GACCAGGGCG CGCGAACACC TACCCAGCCT
    CTGCTGGATT CTGGCTTCCG CTCCGGCAGC CTGGGCCAGC CAAGTCCTTT GGCTCAGAGA
    AACTACCAGA GCTCTTCTCC TCTCCCAACT GCAGGCAGCA GCTACAGCAG CCCCGACTAC
    TCACTGCAGC AGTTCAGCTC CCCAGAAGGC CAGGCCCGGT CTCAGTTCAG TGTGGCCGGT
    GTCCACACAG TGCCTGGGAG CCCCCAGGCA CGGCACAGGA CAGTGGGCAC CAATACACCC
    CCGAGCCCTG GCTTTGGCCG TCGGGCTGTG AACCCCAGCC TGGCTGCCCC CAGCAGCCCC
    AGTTTGAGCC ACCGCCAGGT GATGGGTCCA CTGGGAACCG GTTTCCATGG TAACACGGGC
    TCCAGCCCCC AGAGCAGTGC AGCAACCACT CCGGGAAGCC CCAGTCTGGG TCGCCACCCT
    GGGGCCCACC AGGTTTCCAA CCTCCATGGC AATGTGGTCA CCGCTCCTGG GAGCCCCAGC
    CTGGGCCGGC ACCCCGGGGC CCACCAAGGC ACCCTGGCCT CCAATCTCCA TAGCAATGCA
    GTAGCCAGCC CCGGAAGCCC CAGCCTGGGC CGTCACTTGG GAGGGTCTGG ATCTGTGGTT
    CCCGGCAGCC CCAGCTTGGA CCGGCACGTG CCCTATGGTG GCTACTCCAC CCCTGAGGAC
    CGGAGACCCA CGCTGTCCCG GCAGAGCAGC GCCTCAGGCT ACCAGGCTCC TTCCACGCCC
    TCCTTCCCTG TGTCCCCCGC CTACTACCCT GGCCTGAGCA GCCCCGCCAC CTCCCCGTCG
    CCTGATTCGG CAGCTTTCCG ACAAGGGAGC CCCACCCCGG CCTTGCCGGA GAAGCGGAGG
    ATGTCCATGG GCGACCGAGC GGGCAGCCTC CCCAACTACG CCACCGTCAA CGGGAAGGTG
    TCCTCCTCGC CGGTCGCCAG TGGCATGTCC AGTCCCAGCG GGGGCAGCAC CGTCTCCTTC
    TCCCACACTC TGCCCGACTT CTCCAAGTAC TCCATGCCAG ACAACAGTCC TGAGACGCGG
    GCTAAAGTGA AGTTTGTCCA AGACACTTCC AAGTATTGGT ACAAGCCTGA GATCTCCAGA
    GAACAGGCCA TCGCCCTCCT TAAGGACCAG GAGCCAGGGG CTTTCATCAT CCGTGACAGC
    CACTCCTTCC GAGGAGCCTA TGGGCTGGCC ATGAAGGTGT CTTCGCCCCC TCCGACCATC
    ATGCAGCAGA ATAAAAAAGG GGACATGACC CACGAGCTCG TGAGGCATTT CCTGATAGAG
    ACCGGCCCCA GAGGAGTCAA GCTCAAGGGC TGCCCCAATG AGCCAAACTT CGGATCTCTG
    TCTGCCCTGG TCTACCAGCA CTCCATCATC CCGCTGGCCC TGCCCTGCAA GCTGGTCATT
    CCAAACCGAG ACCCCACAGA TGAATCGAAA GATAGCTCGG GCCCTGCCAA CTCAACCAGT
    GACCTGCTGA AGCAAGGGGC AGCCTGCAAC GTGCTCTTCG TCAACTCTGT GGACATGGAG
    TCACTCACTG GGCCACAGGC CATCTCCAAA GCCATTTCTG AGACCCTGGC TGCTGACCCC
    ACACCAGCTG CCACCATCGT TCACTTCAAA GTTTCTGCCC AGGGAATTAC ACTGACTGAC
    AACCAGAGAA AGCTTTTCTT CAGGCGGCAC TACCCCCTCA ACACTGTGAC CTTCTGTGAC
    CTGGATCCAC AGGAGAGGAA GTGGACAAAG ACGGAGGGAG GTGCCCCTGC TAAGCTCTTC
    GGTTTTGTGG CCCGGAAACA GGGCAGCGCC ACGGACAACG CCTGCCACCT CTTTGCTGAG
    CTTGACCCCA ACCAGCCGGC CTCCGCCATC GTCAGCTTTG TCTCCAAGGT CATGCTGAGT
    GCCGGCCAGA AGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854858.1
    CDS343..5580
    Translation

    Target ORF information:

    RefSeq Version XM_024999090.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999090.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GCGTACAATG CCAGGCTGCA GGGCACTGGG CGGAGAGTCA GCTCCTGGCA CCCTCCTCTC
    CACCGGCTCC GATCTTCCTC TCTCTCTGGG GTGCAGGCTC GGGAGAAGCA GCCTGCAGAG
    CCCCCAGCAC CTCTCCGGAA GCGGGCTGCC AGTGATGGGC AGTATGAGAA CCAGTCACCA
    GAACCCACAT CCCCCCGTAG CCCTGGGGTT CGCTCGCCTG TCCAATGTGT CTCCCCAGAG
    CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGACCCA AAGAGCCCCA TCTGCACAGC
    TACAAGGAAG CCTTTGAGGA GATGGAGGGA ACCTCTCCGA CCAGCCCACC ACCCAGTGGG
    GTGCGTTCCC CTCCAGGTCT GGCCAAGACA CCCTTGTCTG CTCTGGGCCT GAAGCCCCAC
    AACCCAGCCG ACATCCTGCT GCATCCCACT GGTGAGCCCC GGAGCTACGT GGAGTCGGTG
    GTACGAACGG CGGTGGCTGG GCCCCGGACT CAGGAACCTG AGCCCAAGAG CTTTAGCGCC
    CCAGGTGCGC AGGCGTATGG CCATGAGACA CCCCTGAGGA TTGGGACCCT GGGAGGCTCC
    TTTGTCTCCC CTAGCCCCCT CTCCACCAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCA
    GTGGGGAGTT TCCCATCCGG GGAGAGCAGC GACCAGGGCG CGCGAACACC TACCCAGCCT
    CTGCTGGATT CTGGCTTCCG CTCCGGCAGC CTGGGCCAGC CAAGTCCTTT GGCTCAGAGA
    AACTACCAGA GCTCTTCTCC TCTCCCAACT GCAGGCAGCA GCTACAGCAG CCCCGACTAC
    TCACTGCAGC AGTTCAGCTC CCCAGAAGGC CAGGCCCGGT CTCAGTTCAG TGTGGCCGGT
    GTCCACACAG TGCCTGGGAG CCCCCAGGCA CGGCACAGGA CAGTGGGCAC CAATACACCC
    CCGAGCCCTG GCTTTGGCCG TCGGGCTGTG AACCCCAGCC TGGCTGCCCC CAGCAGCCCC
    AGTTTGAGCC ACCGCCAGGT GATGGGTCCA CTGGGAACCG GTTTCCATGG TAACACGGGC
    TCCAGCCCCC AGAGCAGTGC AGCAACCACT CCGGGAAGCC CCAGTCTGGG TCGCCACCCT
    GGGGCCCACC AGGTTTCCAA CCTCCATGGC AATGTGGTCA CCGCTCCTGG GAGCCCCAGC
    CTGGGCCGGC ACCCCGGGGC CCACCAAGGC ACCCTGGCCT CCAATCTCCA TAGCAATGCA
    GTAGCCAGCC CCGGAAGCCC CAGCCTGGGC CGTCACTTGG GAGGGTCTGG ATCTGTGGTT
    CCCGGCAGCC CCAGCTTGGA CCGGCACGTG CCCTATGGTG GCTACTCCAC CCCTGAGGAC
    CGGAGACCCA CGCTGTCCCG GCAGAGCAGC GCCTCAGGCT ACCAGGCTCC TTCCACGCCC
    TCCTTCCCTG TGTCCCCCGC CTACTACCCT GGCCTGAGCA GCCCCGCCAC CTCCCCGTCG
    CCTGATTCGG CAGCTTTCCG ACAAGGGAGC CCCACCCCGG CCTTGCCGGA GAAGCGGAGG
    ATGTCCATGG GCGACCGAGC GGGCAGCCTC CCCAACTACG CCACCGTCAA CGGGAAGGTG
    TCCTCCTCGC CGGTCGCCAG TGGCATGTCC AGTCCCAGCG GGGGCAGCAC CGTCTCCTTC
    TCCCACACTC TGCCCGACTT CTCCAAGTAC TCCATGCCAG ACAACAGTCC TGAGACGCGG
    GCTAAAGTGA AGTTTGTCCA AGACACTTCC AAGTATTGGT ACAAGCCTGA GATCTCCAGA
    GAACAGGCCA TCGCCCTCCT TAAGGACCAG GAGCCAGGGG CTTTCATCAT CCGTGACAGC
    CACTCCTTCC GAGGAGCCTA TGGGCTGGCC ATGAAGGTGT CTTCGCCCCC TCCGACCATC
    ATGCAGCAGA ATAAAAAAGG GGACATGACC CACGAGCTCG TGAGGCATTT CCTGATAGAG
    ACCGGCCCCA GAGGAGTCAA GCTCAAGGGC TGCCCCAATG AGCCAAACTT CGGATCTCTG
    TCTGCCCTGG TCTACCAGCA CTCCATCATC CCGCTGGCCC TGCCCTGCAA GCTGGTCATT
    CCAAACCGAG ACCCCACAGA TGAATCGAAA GATAGCTCGG GCCCTGCCAA CTCAACCAGT
    GACCTGCTGA AGCAAGGGGC AGCCTGCAAC GTGCTCTTCG TCAACTCTGT GGACATGGAG
    TCACTCACTG GGCCACAGGC CATCTCCAAA GCCATTTCTG AGACCCTGGC TGCTGACCCC
    ACACCAGCTG CCACCATCGT TCACTTCAAA GTTTCTGCCC AGGGAATTAC ACTGACTGAC
    AACCAGAGAA AGCTTTTCTT CAGGCGGCAC TACCCCCTCA ACACTGTGAC CTTCTGTGAC
    CTGGATCCAC AGGAGAGGAA GTGGACAAAG ACGGAGGGAG GTGCCCCTGC TAAGCTCTTC
    GGTTTTGTGG CCCGGAAACA GGGCAGCGCC ACGGACAACG CCTGCCACCT CTTTGCTGAG
    CTTGACCCCA ACCAGCCGGC CTCCGCCATC GTCAGCTTTG TCTCCAAGGT CATGCTGAGT
    GCCGGCCAGA AGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227171
    Clone ID Related Accession (Same CDS sequence) XM_024999100.1
    Accession Version XM_024999100.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5676bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGACATGGA TCTGTCTATC CTGCATGCTC TGGCCAGAGG ACCTGGAGGC CCCCAAGACT 
    CACCACTTCA AGGTGAAGAC CTTCAAGAAG GTGAAGCCCT GTGGGATCTG CCGACAGGTC
    ATCACCCGAG AGGGCTGCAC CTGCAAAGTC TGCAGCTTCT CCTGTCATCG GAAATGCCAG
    GCCAAGGTGG CTGCCCCCTG CATTCCTCCT TCCAGCCATG AACTGGTGCC CGTCAACACT
    GAGACTGCTC CGAAGAATGT AGTGGACACG GGAGAAGCAG CCTCTCGGGG TGGAAACACA
    CGGAAAAGCC TTGAGGAAGA TAGTTCCACC AGAGTTACAC CAAGTGTCCA GCCTCATCCC
    CAGCCCATCA GAAACATGAG TACGAGCCGG ACCACGGAGG ACAGCTGTGA GCTGGACCTG
    GTGTATGTCA CCGAGAGGAT CATCGCCGTC TCCTTCCCGA GCACAGCCAA TGAGGAGAAC
    TTCCGGAGCA ACCTCCGAGA GGTGGCCCAG ATGCTCAAGT CCAAACATGG AGGCAACTAC
    CTGCTTTTCA ACCTCTCTGA GCGGAGACCT GACATCACGA AGCTCCACGC CAAGGTCCTG
    GAATTTGGCT GGCCCGACCT CCACACCCCG GCCCTGGAGA AGATCTGCAG CGTCTGCAAG
    GCCATGGACA CCTGGCTCAA TGCAGACCCC CACAACGTGG TCGTGCTACA CAACAAGGGA
    AACCGAGGCA GGATCGGCGT TGTGATCGCG GCTTACATGC ACTACAGCAA CATTTCTGCC
    AGTGCCGACC AGGCCCTGGA CCGGTTTGCG ATGAAACGGT TCTACGAGGA TAAGATTGTA
    CCCATTGGCC AACCATCCCA GAGGAGGTAC GTGCATTATT TCAGCGGCCT GCTCTCTGGC
    TCCATCAAAA TGAACAACAA GCCCTTGTTT CTGCACCACG TGATCATGCA CGGCATCCCC
    AACTTCGAGT CTAAAGGAGG GTGTCGACCA TTTCTCCGGA TCTACCAGGC CATGCAACCT
    GTCTACACAT CTGGCATCTA CAACGTCCAA GGAGACAGCC AGACCAGCAT CTGCATCACC
    ATTGAGCCTG GGCTGCTCTT GAAGGGAGAC ATCTTGCTGA AGTGCTACCA CAAGAAGTTC
    CGGAGCCCAG CCCGAGATGT CATCTTCAGG GTGCAGTTCC ACACCTGTGC CATCCATGAC
    CTGGGAGTTG TCTTTGGGAA GGAGGACCTT GACGATGCCT TCAAGGACGA TCGATTTCCA
    GAATACGGCA AAGTTGAATT TGTATTTTCT TATGGACCAG AGAAAATTCA AGGCATGGAA
    CACCTGGAGA ATGGCCCGAG CGTGTCCGTG GACTATAACA CCTCTGACCC CCTCATCCGC
    TGGGATTCCT ATGACAACTT CAATGGGCAT CGAGATGATG GCATGGAGGA GGTTGTGGGC
    CATACCCAGG GACCCCTGGA CGGGAGCCTG TATGCCAAGG TGAAGAAGAA GGACTCCTTG
    CACGGCAGCA CTGGGGCTGT CAACGCCACG CGGCCCGTGC TGTCAGCCAC CCCCAATCAC
    GTGGAGCACA CCCTCTCCGT GAGCAGTGAC TCGGGCAACT CCACAGCCTC CACCAAGACA
    GACAAGACCG ATGAGCCTGC CCCCGGCCCT GCCAGTGCCC CTGCTGCCCT GAGTCCCGAG
    GAGAAGCGTG AGCTGGACCG CCTGCTCAGT GGCTTTGGCT TGGAGCGGGA GAAGCAAGGC
    GCCATGTACC ACCCTCAGCA TCTCCGGTCC CGCCCAGTGG GAGGCCCTGC TGCCCCTTCC
    TCTGGCCGCC ACATCGTCCC GGCCCAGGTT CACGTCAACG GTGGGGCCTT AGCGTCCGAG
    CGGGAGACAG ACATCCTGGA TGATGAGCTG CCCAACCAGG ATGGGCATAG CGTGGGCAGC
    ATGGGCACCC TGTCCTCCCT GGATGGAGTC ACCAACACAA GTGAGGGTGG CTACCCGGAG
    GCCCTCTCCC CGCTGACCAA CGGTCTGGAC AAGCCCTACC CCATGGAACC TATGGTCAAC
    GGTGGGGGCT ACCCCTATGA GTCTGCCAGC CGGGCAGTCT CTGCCCAGGC TGGCCACACA
    GCCCCCATGA GACCCTCCTA CTCCACACAG GAGGGTTTAG CTGGCTACCA GCGGGAGGGA
    CCCCACCCAG CCTGGCCACA GTCAGCGACC ACCTCCCACT ATGGCCATGA CCCCAACGGT
    ATGTTTCGCT CTCAATCCTT TCCTGAAACG GAGCCCCAGC TGCCCCCAGC TCCAGCCCGT
    GGTGGGAGCA GCCGGGAGGC TGTGCAGAGG GGTCTGAATT CCTGGCAGCA GCAGCAGCAA
    CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCTCGCC AACAGGAAAG AGTCCACTTG
    GAGAGCCTTG GGCTCAGCAG GCCCAGCCCC CAGCCACTAG CAGAGCCCCC CATGTCTGGC
    CTCCCTGAGT TCCCTCGCGC AGCCTCCCAG CAGGAGATCG AGCAGTCCAT CGAAGCCCTC
    AATATGCTGA TGCTCGATTT GGAACCAGCC ACGGCGGGCG CCCCCCTACA CAAGTCCCAG
    AGCGTGCCTG GGGCCTGGCC AGGGGCTTCT CCGCTCTCTT CCCAGCCCTT CTCTGGCTCC
    TCTTGCCAGT CCCACCCACT GACCCAGTCC AGATCTGGCT ACATCCCCAG TGGGCATTCC
    TTGGGAACCC CTGAGCCAGC CCCCCGGGCC CCCCTGGAGT CTGTTCCCAC TGGCAGGCCT
    TACTCACCTT ATGATTATCA GCCATGTCCA ACTGGGCCCA ACCAGAGTTA CCATCCAAAG
    AGCCCGGCCA CCTCCTCCTC GTCTTCCTTC CTTCCAACCA CCCAGAGCTC TGTGGGGCCT
    CAGCAGCCCC CAGCCTCTCT CCCTGGCCTC ACCACTCAGC CTCAGCTTCC ACCAAAGGAG
    GTGACTTCAG ACCCATCCCG AACTCCAGAG GAGGAACCAT TGAACTTGGA AGGGCTGGTG
    GCCCACCGGG TGGCAGCGTA CAATGCCAGG CTGCAGGGCA CTGGGCGGAG AGTCAGCTCC
    TGGCACCCTC CTCTCCACCG GCTCCGATCT TCCTCTCTCT CTGGGGTGCA GGCTCGGGAG
    AAGCAGCCTG CAGAGCCCCC AGCACCTCTC CGGAAGCGGG CTGCCAGTGA TGGGCAGTAT
    GAGAACCAGT CACCAGAACC CACATCCCCC CGTAGCCCTG GGGTTCGCTC GCCTGTCCAA
    TGTGTCTCCC CAGAGCTGGC TCTTACCATC GCTCTCAATC CTGGAGGGCG ACCCAAAGAG
    CCCCATCTGC ACAGCTACAA GGAAGCCTTT GAGGAGATGG AGGGAACCTC TCCGACCAGC
    CCACCACCCA GTGGGGTGCG TTCCCCTCCA GGTCTGGCCA AGACACCCTT GTCTGCTCTG
    GGCCTGAAGC CCCACAACCC AGCCGACATC CTGCTGCATC CCACTGGTGT CCCCAGAAGA
    CTGATCCAGC CAGAGGAGGA TGAGAGGAAG GTGCTGACAG AGCTTTCTGA AGAGCCCCGG
    AGCTACGTGG AGTCGGTGGT ACGAACGGCG GTGGCTGGGC CCCGGACTCA GGAACCTGAG
    CCCAAGAGCT TTAGCGCCCC AGGTGCGCAG GCGTATGGCC ATGAGACACC CCTGAGGATT
    GGGACCCTGG GAGGCTCCTT TGTCTCCCCT AGCCCCCTCT CCACCAGCAG CCCCATCCTC
    AGTGCTGACA GCACTTCAGT GGGGAGTTTC CCATCCGGGG AGAGCAGCGA CCAGGGCGCG
    CGAACACCTA CCCAGCCTCT GCTGGATTCT GGCTTCCGCT CCGGCAGCCT GGGCCAGCCA
    AGTCCTTTGG CTCAGAGAAA CTACCAGAGC TCTTCTCCTC TCCCAACTGC AGGCAGCAGC
    TACAGCAGCC CCGACTACTC ACTGCAGCAG TTCAGCTCCC CAGAAGGCCA GGCCCGGTCT
    CAGTTCAGTG TGGCCGGTGT CCACACAGTG CCTGGGAGCC CCCAGGCACG GCACAGGACA
    GTGGGCACCA ATACACCCCC GAGCCCTGGC TTTGGCCGTC GGGCTGTGAA CCCCAGCCTG
    GCTGCCCCCA GCAGCCCCAG TTTGAGCCAC CGCCAGGTGA TGGGTCCACT GGGAACCGGT
    TTCCATGGTA ACACGGGCTC CAGCCCCCAG AGCAGTGCAG CAACCACTCC GGGAAGCCCC
    AGTCTGGGTC GCCACCCTGG GGCCCACCAG GTTTCCAACC TCCATGGCAA TGTGGTCACC
    GCTCCTGGGA GCCCCAGCCT GGGCCGGCAC CCCGGGGCCC ACCAAGGCAC CCTGGCCTCC
    AATCTCCATA GCAATGCAGT AGCCAGCCCC GGAAGCCCCA GCCTGGGCCG TCACTTGGGA
    GGGTCTGGAT CTGTGGTTCC CGGCAGCCCC AGCTTGGACC GGCACGTGCC CTATGGTGGC
    TACTCCACCC CTGAGGACCG GAGACCCACG CTGTCCCGGC AGAGCAGCGC CTCAGGCTAC
    CAGGCTCCTT CCACGCCCTC CTTCCCTGTG TCCCCCGCCT ACTACCCTGG CCTGAGCAGC
    CCCGCCACCT CCCCGTCGCC TGATTCGGCA GCTTTCCGAC AAGGGAGCCC CACCCCGGCC
    TTGCCGGAGA AGCGGAGGAT GTCCATGGGC GACCGAGCGG GCAGCCTCCC CAACTACGCC
    ACCGTCAACG GGAAGGTGTC CTCCTCGCCG GTCGCCAGTG GCATGTCCAG TCCCAGCGGG
    GGCAGCACCG TCTCCTTCTC CCACACTCTG CCCGACTTCT CCAAGTACTC CATGCCAGAC
    AACAGTCCTG AGACGCGGGC TAAAGTGAAG TTTGTCCAAG ACACTTCCAA GTATTGGTAC
    AAGCCTGAGA TCTCCAGAGA ACAGGCCATC GCCCTCCTTA AGGACCAGGA GCCAGGGGCT
    TTCATCATCC GTGACAGCCA CTCCTTCCGA GGAGCCTATG GGCTGGCCAT GAAGGTGTCT
    TCGCCCCCTC CGACCATCAT GCAGCAGAAT AAAAAAGGGG ACATGACCCA CGAGCTCGTG
    AGGCATTTCC TGATAGAGAC CGGCCCCAGA GGAGTCAAGC TCAAGGGCTG CCCCAATGAG
    CCAAACTTCG GATCTCTGTC TGCCCTGGTC TACCAGCACT CCATCATCCC GCTGGCCCTG
    CCCTGCAAGC TGGTCATTCC AAACCGAGAC CCCACAGATG AATCGAAAGA TAGCTCGGGC
    CCTGCCAACT CAACCAGTGA CCTGCTGAAG CAAGGGGCAG CCTGCAACGT GCTCTTCGTC
    AACTCTGTGG ACATGGAGTC ACTCACTGGG CCACAGGCCA TCTCCAAAGC CATTTCTGAG
    ACCCTGGCTG CTGACCCCAC ACCAGCTGCC ACCATCGTTC ACTTCAAAGT TTCTGCCCAG
    GGAATTACAC TGACTGACAA CCAGAGAAAG CTTTTCTTCA GGCGGCACTA CCCCCTCAAC
    ACTGTGACCT TCTGTGACCT GGATCCACAG GAGAGGAAGT GGACAAAGAC GGAGGGAGGT
    GCCCCTGCTA AGCTCTTCGG TTTTGTGGCC CGGAAACAGG GCAGCGCCAC GGACAACGCC
    TGCCACCTCT TTGCTGAGCT TGACCCCAAC CAGCCGGCCT CCGCCATCGT CAGCTTTGTC
    TCCAAGGTCA TGCTGAGTGC CGGCCAGAAG AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854868.1
    CDS120..5795
    Translation

    Target ORF information:

    RefSeq Version XM_024999100.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999100.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGACATGGA TCTGTCTATC CTGCATGCTC TGGCCAGAGG ACCTGGAGGC CCCCAAGACT 
    CACCACTTCA AGGTGAAGAC CTTCAAGAAG GTGAAGCCCT GTGGGATCTG CCGACAGGTC
    ATCACCCGAG AGGGCTGCAC CTGCAAAGTC TGCAGCTTCT CCTGTCATCG GAAATGCCAG
    GCCAAGGTGG CTGCCCCCTG CATTCCTCCT TCCAGCCATG AACTGGTGCC CGTCAACACT
    GAGACTGCTC CGAAGAATGT AGTGGACACG GGAGAAGCAG CCTCTCGGGG TGGAAACACA
    CGGAAAAGCC TTGAGGAAGA TAGTTCCACC AGAGTTACAC CAAGTGTCCA GCCTCATCCC
    CAGCCCATCA GAAACATGAG TACGAGCCGG ACCACGGAGG ACAGCTGTGA GCTGGACCTG
    GTGTATGTCA CCGAGAGGAT CATCGCCGTC TCCTTCCCGA GCACAGCCAA TGAGGAGAAC
    TTCCGGAGCA ACCTCCGAGA GGTGGCCCAG ATGCTCAAGT CCAAACATGG AGGCAACTAC
    CTGCTTTTCA ACCTCTCTGA GCGGAGACCT GACATCACGA AGCTCCACGC CAAGGTCCTG
    GAATTTGGCT GGCCCGACCT CCACACCCCG GCCCTGGAGA AGATCTGCAG CGTCTGCAAG
    GCCATGGACA CCTGGCTCAA TGCAGACCCC CACAACGTGG TCGTGCTACA CAACAAGGGA
    AACCGAGGCA GGATCGGCGT TGTGATCGCG GCTTACATGC ACTACAGCAA CATTTCTGCC
    AGTGCCGACC AGGCCCTGGA CCGGTTTGCG ATGAAACGGT TCTACGAGGA TAAGATTGTA
    CCCATTGGCC AACCATCCCA GAGGAGGTAC GTGCATTATT TCAGCGGCCT GCTCTCTGGC
    TCCATCAAAA TGAACAACAA GCCCTTGTTT CTGCACCACG TGATCATGCA CGGCATCCCC
    AACTTCGAGT CTAAAGGAGG GTGTCGACCA TTTCTCCGGA TCTACCAGGC CATGCAACCT
    GTCTACACAT CTGGCATCTA CAACGTCCAA GGAGACAGCC AGACCAGCAT CTGCATCACC
    ATTGAGCCTG GGCTGCTCTT GAAGGGAGAC ATCTTGCTGA AGTGCTACCA CAAGAAGTTC
    CGGAGCCCAG CCCGAGATGT CATCTTCAGG GTGCAGTTCC ACACCTGTGC CATCCATGAC
    CTGGGAGTTG TCTTTGGGAA GGAGGACCTT GACGATGCCT TCAAGGACGA TCGATTTCCA
    GAATACGGCA AAGTTGAATT TGTATTTTCT TATGGACCAG AGAAAATTCA AGGCATGGAA
    CACCTGGAGA ATGGCCCGAG CGTGTCCGTG GACTATAACA CCTCTGACCC CCTCATCCGC
    TGGGATTCCT ATGACAACTT CAATGGGCAT CGAGATGATG GCATGGAGGA GGTTGTGGGC
    CATACCCAGG GACCCCTGGA CGGGAGCCTG TATGCCAAGG TGAAGAAGAA GGACTCCTTG
    CACGGCAGCA CTGGGGCTGT CAACGCCACG CGGCCCGTGC TGTCAGCCAC CCCCAATCAC
    GTGGAGCACA CCCTCTCCGT GAGCAGTGAC TCGGGCAACT CCACAGCCTC CACCAAGACA
    GACAAGACCG ATGAGCCTGC CCCCGGCCCT GCCAGTGCCC CTGCTGCCCT GAGTCCCGAG
    GAGAAGCGTG AGCTGGACCG CCTGCTCAGT GGCTTTGGCT TGGAGCGGGA GAAGCAAGGC
    GCCATGTACC ACCCTCAGCA TCTCCGGTCC CGCCCAGTGG GAGGCCCTGC TGCCCCTTCC
    TCTGGCCGCC ACATCGTCCC GGCCCAGGTT CACGTCAACG GTGGGGCCTT AGCGTCCGAG
    CGGGAGACAG ACATCCTGGA TGATGAGCTG CCCAACCAGG ATGGGCATAG CGTGGGCAGC
    ATGGGCACCC TGTCCTCCCT GGATGGAGTC ACCAACACAA GTGAGGGTGG CTACCCGGAG
    GCCCTCTCCC CGCTGACCAA CGGTCTGGAC AAGCCCTACC CCATGGAACC TATGGTCAAC
    GGTGGGGGCT ACCCCTATGA GTCTGCCAGC CGGGCAGTCT CTGCCCAGGC TGGCCACACA
    GCCCCCATGA GACCCTCCTA CTCCACACAG GAGGGTTTAG CTGGCTACCA GCGGGAGGGA
    CCCCACCCAG CCTGGCCACA GTCAGCGACC ACCTCCCACT ATGGCCATGA CCCCAACGGT
    ATGTTTCGCT CTCAATCCTT TCCTGAAACG GAGCCCCAGC TGCCCCCAGC TCCAGCCCGT
    GGTGGGAGCA GCCGGGAGGC TGTGCAGAGG GGTCTGAATT CCTGGCAGCA GCAGCAGCAA
    CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCTCGCC AACAGGAAAG AGTCCACTTG
    GAGAGCCTTG GGCTCAGCAG GCCCAGCCCC CAGCCACTAG CAGAGCCCCC CATGTCTGGC
    CTCCCTGAGT TCCCTCGCGC AGCCTCCCAG CAGGAGATCG AGCAGTCCAT CGAAGCCCTC
    AATATGCTGA TGCTCGATTT GGAACCAGCC ACGGCGGGCG CCCCCCTACA CAAGTCCCAG
    AGCGTGCCTG GGGCCTGGCC AGGGGCTTCT CCGCTCTCTT CCCAGCCCTT CTCTGGCTCC
    TCTTGCCAGT CCCACCCACT GACCCAGTCC AGATCTGGCT ACATCCCCAG TGGGCATTCC
    TTGGGAACCC CTGAGCCAGC CCCCCGGGCC CCCCTGGAGT CTGTTCCCAC TGGCAGGCCT
    TACTCACCTT ATGATTATCA GCCATGTCCA ACTGGGCCCA ACCAGAGTTA CCATCCAAAG
    AGCCCGGCCA CCTCCTCCTC GTCTTCCTTC CTTCCAACCA CCCAGAGCTC TGTGGGGCCT
    CAGCAGCCCC CAGCCTCTCT CCCTGGCCTC ACCACTCAGC CTCAGCTTCC ACCAAAGGAG
    GTGACTTCAG ACCCATCCCG AACTCCAGAG GAGGAACCAT TGAACTTGGA AGGGCTGGTG
    GCCCACCGGG TGGCAGCGTA CAATGCCAGG CTGCAGGGCA CTGGGCGGAG AGTCAGCTCC
    TGGCACCCTC CTCTCCACCG GCTCCGATCT TCCTCTCTCT CTGGGGTGCA GGCTCGGGAG
    AAGCAGCCTG CAGAGCCCCC AGCACCTCTC CGGAAGCGGG CTGCCAGTGA TGGGCAGTAT
    GAGAACCAGT CACCAGAACC CACATCCCCC CGTAGCCCTG GGGTTCGCTC GCCTGTCCAA
    TGTGTCTCCC CAGAGCTGGC TCTTACCATC GCTCTCAATC CTGGAGGGCG ACCCAAAGAG
    CCCCATCTGC ACAGCTACAA GGAAGCCTTT GAGGAGATGG AGGGAACCTC TCCGACCAGC
    CCACCACCCA GTGGGGTGCG TTCCCCTCCA GGTCTGGCCA AGACACCCTT GTCTGCTCTG
    GGCCTGAAGC CCCACAACCC AGCCGACATC CTGCTGCATC CCACTGGTGT CCCCAGAAGA
    CTGATCCAGC CAGAGGAGGA TGAGAGGAAG GTGCTGACAG AGCTTTCTGA AGAGCCCCGG
    AGCTACGTGG AGTCGGTGGT ACGAACGGCG GTGGCTGGGC CCCGGACTCA GGAACCTGAG
    CCCAAGAGCT TTAGCGCCCC AGGTGCGCAG GCGTATGGCC ATGAGACACC CCTGAGGATT
    GGGACCCTGG GAGGCTCCTT TGTCTCCCCT AGCCCCCTCT CCACCAGCAG CCCCATCCTC
    AGTGCTGACA GCACTTCAGT GGGGAGTTTC CCATCCGGGG AGAGCAGCGA CCAGGGCGCG
    CGAACACCTA CCCAGCCTCT GCTGGATTCT GGCTTCCGCT CCGGCAGCCT GGGCCAGCCA
    AGTCCTTTGG CTCAGAGAAA CTACCAGAGC TCTTCTCCTC TCCCAACTGC AGGCAGCAGC
    TACAGCAGCC CCGACTACTC ACTGCAGCAG TTCAGCTCCC CAGAAGGCCA GGCCCGGTCT
    CAGTTCAGTG TGGCCGGTGT CCACACAGTG CCTGGGAGCC CCCAGGCACG GCACAGGACA
    GTGGGCACCA ATACACCCCC GAGCCCTGGC TTTGGCCGTC GGGCTGTGAA CCCCAGCCTG
    GCTGCCCCCA GCAGCCCCAG TTTGAGCCAC CGCCAGGTGA TGGGTCCACT GGGAACCGGT
    TTCCATGGTA ACACGGGCTC CAGCCCCCAG AGCAGTGCAG CAACCACTCC GGGAAGCCCC
    AGTCTGGGTC GCCACCCTGG GGCCCACCAG GTTTCCAACC TCCATGGCAA TGTGGTCACC
    GCTCCTGGGA GCCCCAGCCT GGGCCGGCAC CCCGGGGCCC ACCAAGGCAC CCTGGCCTCC
    AATCTCCATA GCAATGCAGT AGCCAGCCCC GGAAGCCCCA GCCTGGGCCG TCACTTGGGA
    GGGTCTGGAT CTGTGGTTCC CGGCAGCCCC AGCTTGGACC GGCACGTGCC CTATGGTGGC
    TACTCCACCC CTGAGGACCG GAGACCCACG CTGTCCCGGC AGAGCAGCGC CTCAGGCTAC
    CAGGCTCCTT CCACGCCCTC CTTCCCTGTG TCCCCCGCCT ACTACCCTGG CCTGAGCAGC
    CCCGCCACCT CCCCGTCGCC TGATTCGGCA GCTTTCCGAC AAGGGAGCCC CACCCCGGCC
    TTGCCGGAGA AGCGGAGGAT GTCCATGGGC GACCGAGCGG GCAGCCTCCC CAACTACGCC
    ACCGTCAACG GGAAGGTGTC CTCCTCGCCG GTCGCCAGTG GCATGTCCAG TCCCAGCGGG
    GGCAGCACCG TCTCCTTCTC CCACACTCTG CCCGACTTCT CCAAGTACTC CATGCCAGAC
    AACAGTCCTG AGACGCGGGC TAAAGTGAAG TTTGTCCAAG ACACTTCCAA GTATTGGTAC
    AAGCCTGAGA TCTCCAGAGA ACAGGCCATC GCCCTCCTTA AGGACCAGGA GCCAGGGGCT
    TTCATCATCC GTGACAGCCA CTCCTTCCGA GGAGCCTATG GGCTGGCCAT GAAGGTGTCT
    TCGCCCCCTC CGACCATCAT GCAGCAGAAT AAAAAAGGGG ACATGACCCA CGAGCTCGTG
    AGGCATTTCC TGATAGAGAC CGGCCCCAGA GGAGTCAAGC TCAAGGGCTG CCCCAATGAG
    CCAAACTTCG GATCTCTGTC TGCCCTGGTC TACCAGCACT CCATCATCCC GCTGGCCCTG
    CCCTGCAAGC TGGTCATTCC AAACCGAGAC CCCACAGATG AATCGAAAGA TAGCTCGGGC
    CCTGCCAACT CAACCAGTGA CCTGCTGAAG CAAGGGGCAG CCTGCAACGT GCTCTTCGTC
    AACTCTGTGG ACATGGAGTC ACTCACTGGG CCACAGGCCA TCTCCAAAGC CATTTCTGAG
    ACCCTGGCTG CTGACCCCAC ACCAGCTGCC ACCATCGTTC ACTTCAAAGT TTCTGCCCAG
    GGAATTACAC TGACTGACAA CCAGAGAAAG CTTTTCTTCA GGCGGCACTA CCCCCTCAAC
    ACTGTGACCT TCTGTGACCT GGATCCACAG GAGAGGAAGT GGACAAAGAC GGAGGGAGGT
    GCCCCTGCTA AGCTCTTCGG TTTTGTGGCC CGGAAACAGG GCAGCGCCAC GGACAACGCC
    TGCCACCTCT TTGCTGAGCT TGACCCCAAC CAGCCGGCCT CCGCCATCGT CAGCTTTGTC
    TCCAAGGTCA TGCTGAGTGC CGGCCAGAAG AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227172
    Clone ID Related Accession (Same CDS sequence) XM_024999101.1
    Accession Version XM_024999101.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5214bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GGGGTGCAGG CTCGGGAGAA GCAGCCTGCA GAGCCCCCAG CACCTCTCCG GAAGCGGGCT
    GCCAGTGATG GGCAGTATGA GAACCAGTCA CCAGAACCCA CATCCCCCCG TAGCCCTGGG
    GTTCGCTCGC CTGTCCAATG TGTCTCCCCA GAGCTGGCTC TTACCATCGC TCTCAATCCT
    GGAGGGCGAC CCAAAGAGCC CCATCTGCAC AGCTACAAGG AAGCCTTTGA GGAGATGGAG
    GGAACCTCTC CGACCAGCCC ACCACCCAGT GGGGTGCGTT CCCCTCCAGG TCTGGCCAAG
    ACACCCTTGT CTGCTCTGGG CCTGAAGCCC CACAACCCAG CCGACATCCT GCTGCATCCC
    ACTGGTGTCC CCAGAAGACT GATCCAGCCA GAGGAGGATG AGAGGAAGGT GCTGACAGAG
    CTTTCTGAAG AGCCCCGGAG CTACGTGGAG TCGGTGGTAC GAACGGCGGT GGCTGGGCCC
    CGGACTCAGG AACCTGAGCC CAAGAGCTTT AGCGCCCCAG GTGCGCAGGC GTATGGCCAT
    GAGACACCCC TGAGGATTGG GACCCTGGGA GGCTCCTTTG TCTCCCCTAG CCCCCTCTCC
    ACCAGCAGCC CCATCCTCAG TGCTGACAGC ACTTCAGTGG GGAGTTTCCC ATCCGGGGAG
    AGCAGCGACC AGGGCGCGCG AACACCTACC CAGCCTCTGC TGGATTCTGG CTTCCGCTCC
    GGCAGCCTGG GCCAGCCAAG TCCTTTGGCT CAGAGAAACT ACCAGAGCTC TTCTCCTCTC
    CCAACTGCAG GCAGCAGCTA CAGCAGCCCC GACTACTCAC TGCAGCAGTT CAGCTCCCCA
    GAAGGCCAGG CCCGGTCTCA GTTCAGTGTG GCCGGTGTCC ACACAGTGCC TGGGAGCCCC
    CAGGCACGGC ACAGGACAGT GGGCACCAAT ACACCCCCGA GCCCTGGCTT TGGCCGTCGG
    GCTGTGAACC CCAGCCTGGC TGCCCCCAGC AGCCCCAGTT TGAGCCACCG CCAGGTGATG
    GGTCCACTGG GAACCGGTTT CCATGGTAAC ACGGGCTCCA GCCCCCAGAG CAGTGCAGCA
    ACCACTCCGG GAAGCCCCAG TCTGGGTCGC CACCCTGGGG CCCACCAGGT TTCCAACCTC
    CATGGCAATG TGGTCACCGC TCCTGGGAGC CCCAGCCTGG GCCGGCACCC CGGGGCCCAC
    CAAGGCACCC TGGCCTCCAA TCTCCATAGC AATGCAGTAG CCAGCCCCGG AAGCCCCAGC
    CTGGGCCGTC ACTTGGGAGG GTCTGGATCT GTGGTTCCCG GCAGCCCCAG CTTGGACCGG
    CACGTGCCCT ATGGTGGCTA CTCCACCCCT GAGGACCGGA GACCCACGCT GTCCCGGCAG
    AGCAGCGCCT CAGGCTACCA GGCTCCTTCC ACGCCCTCCT TCCCTGTGTC CCCCGCCTAC
    TACCCTGGCC TGAGCAGCCC CGCCACCTCC CCGTCGCCTG ATTCGGCAGC TTTCCGACAA
    GGGAGCCCCA CCCCGGCCTT GCCGGAGAAG CGGAGGATGT CCATGGGCGA CCGAGCGGGC
    AGCCTCCCCA ACTACGCCAC CGTCAACGGG AAGGTGTCCT CCTCGCCGGT CGCCAGTGGC
    ATGTCCAGTC CCAGCGGGGG CAGCACCGTC TCCTTCTCCC ACACTCTGCC CGACTTCTCC
    AAGTACTCCA TGCCAGACAA CAGTCCTGAG ACGCGGGCTA AAGTGAAGTT TGTCCAAGAC
    ACTTCCAAGT ATTGGTACAA GCCTGAGATC TCCAGAGAAC AGGCCATCGC CCTCCTTAAG
    GACCAGGAGC CAGGGGCTTT CATCATCCGT GACAGCCACT CCTTCCGAGG AGCCTATGGG
    CTGGCCATGA AGGTGTCTTC GCCCCCTCCG ACCATCATGC AGCAGAATAA AAAAGGGGAC
    ATGACCCACG AGCTCGTGAG GCATTTCCTG ATAGAGACCG GCCCCAGAGG AGTCAAGCTC
    AAGGGCTGCC CCAATGAGCC AAACTTCGGA TCTCTGTCTG CCCTGGTCTA CCAGCACTCC
    ATCATCCCGC TGGCCCTGCC CTGCAAGCTG GTCATTCCAA ACCGAGACCC CACAGATGAA
    TCGAAAGATA GCTCGGGCCC TGCCAACTCA ACCAGTGACC TGCTGAAGCA AGGGGCAGCC
    TGCAACGTGC TCTTCGTCAA CTCTGTGGAC ATGGAGTCAC TCACTGGGCC ACAGGCCATC
    TCCAAAGCCA TTTCTGAGAC CCTGGCTGCT GACCCCACAC CAGCTGCCAC CATCGTTCAC
    TTCAAAGTTT CTGCCCAGGG AATTACACTG ACTGACAACC AGAGAAAGCT TTTCTTCAGG
    CGGCACTACC CCCTCAACAC TGTGACCTTC TGTGACCTGG ATCCACAGGA GAGGAAGTGG
    ACAAAGACGG AGGGAGGTGC CCCTGCTAAG CTCTTCGGTT TTGTGGCCCG GAAACAGGGC
    AGCGCCACGG ACAACGCCTG CCACCTCTTT GCTGAGCTTG ACCCCAACCA GCCGGCCTCC
    GCCATCGTCA GCTTTGTCTC CAAGGTCATG CTGAGTGCCG GCCAGAAGAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854869.1
    CDS343..5556
    Translation

    Target ORF information:

    RefSeq Version XM_024999101.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999101.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GGGGTGCAGG CTCGGGAGAA GCAGCCTGCA GAGCCCCCAG CACCTCTCCG GAAGCGGGCT
    GCCAGTGATG GGCAGTATGA GAACCAGTCA CCAGAACCCA CATCCCCCCG TAGCCCTGGG
    GTTCGCTCGC CTGTCCAATG TGTCTCCCCA GAGCTGGCTC TTACCATCGC TCTCAATCCT
    GGAGGGCGAC CCAAAGAGCC CCATCTGCAC AGCTACAAGG AAGCCTTTGA GGAGATGGAG
    GGAACCTCTC CGACCAGCCC ACCACCCAGT GGGGTGCGTT CCCCTCCAGG TCTGGCCAAG
    ACACCCTTGT CTGCTCTGGG CCTGAAGCCC CACAACCCAG CCGACATCCT GCTGCATCCC
    ACTGGTGTCC CCAGAAGACT GATCCAGCCA GAGGAGGATG AGAGGAAGGT GCTGACAGAG
    CTTTCTGAAG AGCCCCGGAG CTACGTGGAG TCGGTGGTAC GAACGGCGGT GGCTGGGCCC
    CGGACTCAGG AACCTGAGCC CAAGAGCTTT AGCGCCCCAG GTGCGCAGGC GTATGGCCAT
    GAGACACCCC TGAGGATTGG GACCCTGGGA GGCTCCTTTG TCTCCCCTAG CCCCCTCTCC
    ACCAGCAGCC CCATCCTCAG TGCTGACAGC ACTTCAGTGG GGAGTTTCCC ATCCGGGGAG
    AGCAGCGACC AGGGCGCGCG AACACCTACC CAGCCTCTGC TGGATTCTGG CTTCCGCTCC
    GGCAGCCTGG GCCAGCCAAG TCCTTTGGCT CAGAGAAACT ACCAGAGCTC TTCTCCTCTC
    CCAACTGCAG GCAGCAGCTA CAGCAGCCCC GACTACTCAC TGCAGCAGTT CAGCTCCCCA
    GAAGGCCAGG CCCGGTCTCA GTTCAGTGTG GCCGGTGTCC ACACAGTGCC TGGGAGCCCC
    CAGGCACGGC ACAGGACAGT GGGCACCAAT ACACCCCCGA GCCCTGGCTT TGGCCGTCGG
    GCTGTGAACC CCAGCCTGGC TGCCCCCAGC AGCCCCAGTT TGAGCCACCG CCAGGTGATG
    GGTCCACTGG GAACCGGTTT CCATGGTAAC ACGGGCTCCA GCCCCCAGAG CAGTGCAGCA
    ACCACTCCGG GAAGCCCCAG TCTGGGTCGC CACCCTGGGG CCCACCAGGT TTCCAACCTC
    CATGGCAATG TGGTCACCGC TCCTGGGAGC CCCAGCCTGG GCCGGCACCC CGGGGCCCAC
    CAAGGCACCC TGGCCTCCAA TCTCCATAGC AATGCAGTAG CCAGCCCCGG AAGCCCCAGC
    CTGGGCCGTC ACTTGGGAGG GTCTGGATCT GTGGTTCCCG GCAGCCCCAG CTTGGACCGG
    CACGTGCCCT ATGGTGGCTA CTCCACCCCT GAGGACCGGA GACCCACGCT GTCCCGGCAG
    AGCAGCGCCT CAGGCTACCA GGCTCCTTCC ACGCCCTCCT TCCCTGTGTC CCCCGCCTAC
    TACCCTGGCC TGAGCAGCCC CGCCACCTCC CCGTCGCCTG ATTCGGCAGC TTTCCGACAA
    GGGAGCCCCA CCCCGGCCTT GCCGGAGAAG CGGAGGATGT CCATGGGCGA CCGAGCGGGC
    AGCCTCCCCA ACTACGCCAC CGTCAACGGG AAGGTGTCCT CCTCGCCGGT CGCCAGTGGC
    ATGTCCAGTC CCAGCGGGGG CAGCACCGTC TCCTTCTCCC ACACTCTGCC CGACTTCTCC
    AAGTACTCCA TGCCAGACAA CAGTCCTGAG ACGCGGGCTA AAGTGAAGTT TGTCCAAGAC
    ACTTCCAAGT ATTGGTACAA GCCTGAGATC TCCAGAGAAC AGGCCATCGC CCTCCTTAAG
    GACCAGGAGC CAGGGGCTTT CATCATCCGT GACAGCCACT CCTTCCGAGG AGCCTATGGG
    CTGGCCATGA AGGTGTCTTC GCCCCCTCCG ACCATCATGC AGCAGAATAA AAAAGGGGAC
    ATGACCCACG AGCTCGTGAG GCATTTCCTG ATAGAGACCG GCCCCAGAGG AGTCAAGCTC
    AAGGGCTGCC CCAATGAGCC AAACTTCGGA TCTCTGTCTG CCCTGGTCTA CCAGCACTCC
    ATCATCCCGC TGGCCCTGCC CTGCAAGCTG GTCATTCCAA ACCGAGACCC CACAGATGAA
    TCGAAAGATA GCTCGGGCCC TGCCAACTCA ACCAGTGACC TGCTGAAGCA AGGGGCAGCC
    TGCAACGTGC TCTTCGTCAA CTCTGTGGAC ATGGAGTCAC TCACTGGGCC ACAGGCCATC
    TCCAAAGCCA TTTCTGAGAC CCTGGCTGCT GACCCCACAC CAGCTGCCAC CATCGTTCAC
    TTCAAAGTTT CTGCCCAGGG AATTACACTG ACTGACAACC AGAGAAAGCT TTTCTTCAGG
    CGGCACTACC CCCTCAACAC TGTGACCTTC TGTGACCTGG ATCCACAGGA GAGGAAGTGG
    ACAAAGACGG AGGGAGGTGC CCCTGCTAAG CTCTTCGGTT TTGTGGCCCG GAAACAGGGC
    AGCGCCACGG ACAACGCCTG CCACCTCTTT GCTGAGCTTG ACCCCAACCA GCCGGCCTCC
    GCCATCGTCA GCTTTGTCTC CAAGGTCATG CTGAGTGCCG GCCAGAAGAG ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227173
    Clone ID Related Accession (Same CDS sequence) XM_024999105.1
    Accession Version XM_024999105.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GGGGTGCAGG CTCGGGAGAA GCAGCCTGCA GAGCCCCCAG CACCTCTCCG GAAGCGGGCT
    GCCAGTGATG GGCAGTATGA GAACCAGTCA CCAGAACCCA CATCCCCCCG TAGCCCTGGG
    GTTCGCTCGC CTGTCCAATG TGTCTCCCCA GAGCTGGCTC TTACCATCGC TCTCAATCCT
    GGAGGGCGAC CCAAAGAGCC CCATCTGCAC AGCTACAAGG AAGCCTTTGA GGAGATGGAG
    GGAACCTCTC CGACCAGCCC ACCACCCAGT GGGGTGCGTT CCCCTCCAGG TCTGGCCAAG
    ACACCCTTGT CTGCTCTGGG CCTGAAGCCC CACAACCCAG CCGACATCCT GCTGCATCCC
    ACTGGTGAGG AGGATGAGAG GAAGGTGCTG ACAGAGCTTT CTGAAGAGCC CCGGAGCTAC
    GTGGAGTCGG TGGTACGAAC GGCGGTGGCT GGGCCCCGGA CTCAGGAACC TGAGCCCAAG
    AGCTTTAGCG CCCCAGGTGC GCAGGCGTAT GGCCATGAGA CACCCCTGAG GATTGGGACC
    CTGGGAGGCT CCTTTGTCTC CCCTAGCCCC CTCTCCACCA GCAGCCCCAT CCTCAGTGCT
    GACAGCACTT CAGTGGGGAG TTTCCCATCC GGGGAGAGCA GCGACCAGGG CGCGCGAACA
    CCTACCCAGC CTCTGCTGGA TTCTGGCTTC CGCTCCGGCA GCCTGGGCCA GCCAAGTCCT
    TTGGCTCAGA GAAACTACCA GAGCTCTTCT CCTCTCCCAA CTGCAGGCAG CAGCTACAGC
    AGCCCCGACT ACTCACTGCA GCAGTTCAGC TCCCCAGAAG GCCAGGCCCG GTCTCAGTTC
    AGTGTGGCCG GTGTCCACAC AGTGCCTGGG AGCCCCCAGG CACGGCACAG GACAGTGGGC
    ACCAATACAC CCCCGAGCCC TGGCTTTGGC CGTCGGGCTG TGAACCCCAG CCTGGCTGCC
    CCCAGCAGCC CCAGTTTGAG CCACCGCCAG GTGATGGGTC CACTGGGAAC CGGTTTCCAT
    GGTAACACGG GCTCCAGCCC CCAGAGCAGT GCAGCAACCA CTCCGGGAAG CCCCAGTCTG
    GGTCGCCACC CTGGGGCCCA CCAGGTTTCC AACCTCCATG GCAATGTGGT CACCGCTCCT
    GGGAGCCCCA GCCTGGGCCG GCACCCCGGG GCCCACCAAG GCACCCTGGC CTCCAATCTC
    CATAGCAATG CAGTAGCCAG CCCCGGAAGC CCCAGCCTGG GCCGTCACTT GGGAGGGTCT
    GGATCTGTGG TTCCCGGCAG CCCCAGCTTG GACCGGCACG TGCCCTATGG TGGCTACTCC
    ACCCCTGAGG ACCGGAGACC CACGCTGTCC CGGCAGAGCA GCGCCTCAGG CTACCAGGCT
    CCTTCCACGC CCTCCTTCCC TGTGTCCCCC GCCTACTACC CTGGCCTGAG CAGCCCCGCC
    ACCTCCCCGT CGCCTGATTC GGCAGCTTTC CGACAAGGGA GCCCCACCCC GGCCTTGCCG
    GAGAAGCGGA GGATGTCCAT GGGCGACCGA GCGGGCAGCC TCCCCAACTA CGCCACCGTC
    AACGGGAAGG TGTCCTCCTC GCCGGTCGCC AGTGGCATGT CCAGTCCCAG CGGGGGCAGC
    ACCGTCTCCT TCTCCCACAC TCTGCCCGAC TTCTCCAAGT ACTCCATGCC AGACAACAGT
    CCTGAGACGC GGGCTAAAGT GAAGTTTGTC CAAGACACTT CCAAGTATTG GTACAAGCCT
    GAGATCTCCA GAGAACAGGC CATCGCCCTC CTTAAGGACC AGGAGCCAGG GGCTTTCATC
    ATCCGTGACA GCCACTCCTT CCGAGGAGCC TATGGGCTGG CCATGAAGGT GTCTTCGCCC
    CCTCCGACCA TCATGCAGCA GAATAAAAAA GGGGACATGA CCCACGAGCT CGTGAGGCAT
    TTCCTGATAG AGACCGGCCC CAGAGGAGTC AAGCTCAAGG GCTGCCCCAA TGAGCCAAAC
    TTCGGATCTC TGTCTGCCCT GGTCTACCAG CACTCCATCA TCCCGCTGGC CCTGCCCTGC
    AAGCTGGTCA TTCCAAACCG AGACCCCACA GATGAATCGA AAGATAGCTC GGGCCCTGCC
    AACTCAACCA GTGACCTGCT GAAGCAAGGG GCAGCCTGCA ACGTGCTCTT CGTCAACTCT
    GTGGACATGG AGTCACTCAC TGGGCCACAG GCCATCTCCA AAGCCATTTC TGAGACCCTG
    GCTGCTGACC CCACACCAGC TGCCACCATC GTTCACTTCA AAGTTTCTGC CCAGGGAATT
    ACACTGACTG ACAACCAGAG AAAGCTTTTC TTCAGGCGGC ACTACCCCCT CAACACTGTG
    ACCTTCTGTG ACCTGGATCC ACAGGAGAGG AAGTGGACAA AGACGGAGGG AGGTGCCCCT
    GCTAAGCTCT TCGGTTTTGT GGCCCGGAAA CAGGGCAGCG CCACGGACAA CGCCTGCCAC
    CTCTTTGCTG AGCTTGACCC CAACCAGCCG GCCTCCGCCA TCGTCAGCTT TGTCTCCAAG
    GTCATGCTGA GTGCCGGCCA GAAGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854873.1
    CDS343..5532
    Translation

    Target ORF information:

    RefSeq Version XM_024999105.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999105.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GGGGTGCAGG CTCGGGAGAA GCAGCCTGCA GAGCCCCCAG CACCTCTCCG GAAGCGGGCT
    GCCAGTGATG GGCAGTATGA GAACCAGTCA CCAGAACCCA CATCCCCCCG TAGCCCTGGG
    GTTCGCTCGC CTGTCCAATG TGTCTCCCCA GAGCTGGCTC TTACCATCGC TCTCAATCCT
    GGAGGGCGAC CCAAAGAGCC CCATCTGCAC AGCTACAAGG AAGCCTTTGA GGAGATGGAG
    GGAACCTCTC CGACCAGCCC ACCACCCAGT GGGGTGCGTT CCCCTCCAGG TCTGGCCAAG
    ACACCCTTGT CTGCTCTGGG CCTGAAGCCC CACAACCCAG CCGACATCCT GCTGCATCCC
    ACTGGTGAGG AGGATGAGAG GAAGGTGCTG ACAGAGCTTT CTGAAGAGCC CCGGAGCTAC
    GTGGAGTCGG TGGTACGAAC GGCGGTGGCT GGGCCCCGGA CTCAGGAACC TGAGCCCAAG
    AGCTTTAGCG CCCCAGGTGC GCAGGCGTAT GGCCATGAGA CACCCCTGAG GATTGGGACC
    CTGGGAGGCT CCTTTGTCTC CCCTAGCCCC CTCTCCACCA GCAGCCCCAT CCTCAGTGCT
    GACAGCACTT CAGTGGGGAG TTTCCCATCC GGGGAGAGCA GCGACCAGGG CGCGCGAACA
    CCTACCCAGC CTCTGCTGGA TTCTGGCTTC CGCTCCGGCA GCCTGGGCCA GCCAAGTCCT
    TTGGCTCAGA GAAACTACCA GAGCTCTTCT CCTCTCCCAA CTGCAGGCAG CAGCTACAGC
    AGCCCCGACT ACTCACTGCA GCAGTTCAGC TCCCCAGAAG GCCAGGCCCG GTCTCAGTTC
    AGTGTGGCCG GTGTCCACAC AGTGCCTGGG AGCCCCCAGG CACGGCACAG GACAGTGGGC
    ACCAATACAC CCCCGAGCCC TGGCTTTGGC CGTCGGGCTG TGAACCCCAG CCTGGCTGCC
    CCCAGCAGCC CCAGTTTGAG CCACCGCCAG GTGATGGGTC CACTGGGAAC CGGTTTCCAT
    GGTAACACGG GCTCCAGCCC CCAGAGCAGT GCAGCAACCA CTCCGGGAAG CCCCAGTCTG
    GGTCGCCACC CTGGGGCCCA CCAGGTTTCC AACCTCCATG GCAATGTGGT CACCGCTCCT
    GGGAGCCCCA GCCTGGGCCG GCACCCCGGG GCCCACCAAG GCACCCTGGC CTCCAATCTC
    CATAGCAATG CAGTAGCCAG CCCCGGAAGC CCCAGCCTGG GCCGTCACTT GGGAGGGTCT
    GGATCTGTGG TTCCCGGCAG CCCCAGCTTG GACCGGCACG TGCCCTATGG TGGCTACTCC
    ACCCCTGAGG ACCGGAGACC CACGCTGTCC CGGCAGAGCA GCGCCTCAGG CTACCAGGCT
    CCTTCCACGC CCTCCTTCCC TGTGTCCCCC GCCTACTACC CTGGCCTGAG CAGCCCCGCC
    ACCTCCCCGT CGCCTGATTC GGCAGCTTTC CGACAAGGGA GCCCCACCCC GGCCTTGCCG
    GAGAAGCGGA GGATGTCCAT GGGCGACCGA GCGGGCAGCC TCCCCAACTA CGCCACCGTC
    AACGGGAAGG TGTCCTCCTC GCCGGTCGCC AGTGGCATGT CCAGTCCCAG CGGGGGCAGC
    ACCGTCTCCT TCTCCCACAC TCTGCCCGAC TTCTCCAAGT ACTCCATGCC AGACAACAGT
    CCTGAGACGC GGGCTAAAGT GAAGTTTGTC CAAGACACTT CCAAGTATTG GTACAAGCCT
    GAGATCTCCA GAGAACAGGC CATCGCCCTC CTTAAGGACC AGGAGCCAGG GGCTTTCATC
    ATCCGTGACA GCCACTCCTT CCGAGGAGCC TATGGGCTGG CCATGAAGGT GTCTTCGCCC
    CCTCCGACCA TCATGCAGCA GAATAAAAAA GGGGACATGA CCCACGAGCT CGTGAGGCAT
    TTCCTGATAG AGACCGGCCC CAGAGGAGTC AAGCTCAAGG GCTGCCCCAA TGAGCCAAAC
    TTCGGATCTC TGTCTGCCCT GGTCTACCAG CACTCCATCA TCCCGCTGGC CCTGCCCTGC
    AAGCTGGTCA TTCCAAACCG AGACCCCACA GATGAATCGA AAGATAGCTC GGGCCCTGCC
    AACTCAACCA GTGACCTGCT GAAGCAAGGG GCAGCCTGCA ACGTGCTCTT CGTCAACTCT
    GTGGACATGG AGTCACTCAC TGGGCCACAG GCCATCTCCA AAGCCATTTC TGAGACCCTG
    GCTGCTGACC CCACACCAGC TGCCACCATC GTTCACTTCA AAGTTTCTGC CCAGGGAATT
    ACACTGACTG ACAACCAGAG AAAGCTTTTC TTCAGGCGGC ACTACCCCCT CAACACTGTG
    ACCTTCTGTG ACCTGGATCC ACAGGAGAGG AAGTGGACAA AGACGGAGGG AGGTGCCCCT
    GCTAAGCTCT TCGGTTTTGT GGCCCGGAAA CAGGGCAGCG CCACGGACAA CGCCTGCCAC
    CTCTTTGCTG AGCTTGACCC CAACCAGCCG GCCTCCGCCA TCGTCAGCTT TGTCTCCAAG
    GTCATGCTGA GTGCCGGCCA GAAGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227174
    Clone ID Related Accession (Same CDS sequence) XM_024999109.1 , XM_024999158.1
    Accession Version XM_024999109.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GGGGTGCAGG CTCGGGAGAA GCAGCCTGCA GAGCCCCCAG CACCTCTCCG GAAGCGGGCT
    GCCAGTGATG GGCAGTATGA GAACCAGTCA CCAGAACCCA CATCCCCCCG TAGCCCTGGG
    GTTCGCTCGC CTGTCCAATG TGTCTCCCCA GAGCTGGCTC TTACCATCGC TCTCAATCCT
    GGAGGGCGAC CCAAAGAGCC CCATCTGCAC AGCTACAAGG AAGCCTTTGA GGAGATGGAG
    GGAACCTCTC CGACCAGCCC ACCACCCAGT GGGGTGCGTT CCCCTCCAGG TCTGGCCAAG
    ACACCCTTGT CTGCTCTGGG CCTGAAGCCC CACAACCCAG CCGACATCCT GCTGCATCCC
    ACTGGTGTCC CCAGAAGACT GATCCAGCCA GAGCCCCGGA GCTACGTGGA GTCGGTGGTA
    CGAACGGCGG TGGCTGGGCC CCGGACTCAG GAACCTGAGC CCAAGAGCTT TAGCGCCCCA
    GGTGCGCAGG CGTATGGCCA TGAGACACCC CTGAGGATTG GGACCCTGGG AGGCTCCTTT
    GTCTCCCCTA GCCCCCTCTC CACCAGCAGC CCCATCCTCA GTGCTGACAG CACTTCAGTG
    GGGAGTTTCC CATCCGGGGA GAGCAGCGAC CAGGGCGCGC GAACACCTAC CCAGCCTCTG
    CTGGATTCTG GCTTCCGCTC CGGCAGCCTG GGCCAGCCAA GTCCTTTGGC TCAGAGAAAC
    TACCAGAGCT CTTCTCCTCT CCCAACTGCA GGCAGCAGCT ACAGCAGCCC CGACTACTCA
    CTGCAGCAGT TCAGCTCCCC AGAAGGCCAG GCCCGGTCTC AGTTCAGTGT GGCCGGTGTC
    CACACAGTGC CTGGGAGCCC CCAGGCACGG CACAGGACAG TGGGCACCAA TACACCCCCG
    AGCCCTGGCT TTGGCCGTCG GGCTGTGAAC CCCAGCCTGG CTGCCCCCAG CAGCCCCAGT
    TTGAGCCACC GCCAGGTGAT GGGTCCACTG GGAACCGGTT TCCATGGTAA CACGGGCTCC
    AGCCCCCAGA GCAGTGCAGC AACCACTCCG GGAAGCCCCA GTCTGGGTCG CCACCCTGGG
    GCCCACCAGG TTTCCAACCT CCATGGCAAT GTGGTCACCG CTCCTGGGAG CCCCAGCCTG
    GGCCGGCACC CCGGGGCCCA CCAAGGCACC CTGGCCTCCA ATCTCCATAG CAATGCAGTA
    GCCAGCCCCG GAAGCCCCAG CCTGGGCCGT CACTTGGGAG GGTCTGGATC TGTGGTTCCC
    GGCAGCCCCA GCTTGGACCG GCACGTGCCC TATGGTGGCT ACTCCACCCC TGAGGACCGG
    AGACCCACGC TGTCCCGGCA GAGCAGCGCC TCAGGCTACC AGGCTCCTTC CACGCCCTCC
    TTCCCTGTGT CCCCCGCCTA CTACCCTGGC CTGAGCAGCC CCGCCACCTC CCCGTCGCCT
    GATTCGGCAG CTTTCCGACA AGGGAGCCCC ACCCCGGCCT TGCCGGAGAA GCGGAGGATG
    TCCATGGGCG ACCGAGCGGG CAGCCTCCCC AACTACGCCA CCGTCAACGG GAAGGTGTCC
    TCCTCGCCGG TCGCCAGTGG CATGTCCAGT CCCAGCGGGG GCAGCACCGT CTCCTTCTCC
    CACACTCTGC CCGACTTCTC CAAGTACTCC ATGCCAGACA ACAGTCCTGA GACGCGGGCT
    AAAGTGAAGT TTGTCCAAGA CACTTCCAAG TATTGGTACA AGCCTGAGAT CTCCAGAGAA
    CAGGCCATCG CCCTCCTTAA GGACCAGGAG CCAGGGGCTT TCATCATCCG TGACAGCCAC
    TCCTTCCGAG GAGCCTATGG GCTGGCCATG AAGGTGTCTT CGCCCCCTCC GACCATCATG
    CAGCAGAATA AAAAAGGGGA CATGACCCAC GAGCTCGTGA GGCATTTCCT GATAGAGACC
    GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC CCCAATGAGC CAAACTTCGG ATCTCTGTCT
    GCCCTGGTCT ACCAGCACTC CATCATCCCG CTGGCCCTGC CCTGCAAGCT GGTCATTCCA
    AACCGAGACC CCACAGATGA ATCGAAAGAT AGCTCGGGCC CTGCCAACTC AACCAGTGAC
    CTGCTGAAGC AAGGGGCAGC CTGCAACGTG CTCTTCGTCA ACTCTGTGGA CATGGAGTCA
    CTCACTGGGC CACAGGCCAT CTCCAAAGCC ATTTCTGAGA CCCTGGCTGC TGACCCCACA
    CCAGCTGCCA CCATCGTTCA CTTCAAAGTT TCTGCCCAGG GAATTACACT GACTGACAAC
    CAGAGAAAGC TTTTCTTCAG GCGGCACTAC CCCCTCAACA CTGTGACCTT CTGTGACCTG
    GATCCACAGG AGAGGAAGTG GACAAAGACG GAGGGAGGTG CCCCTGCTAA GCTCTTCGGT
    TTTGTGGCCC GGAAACAGGG CAGCGCCACG GACAACGCCT GCCACCTCTT TGCTGAGCTT
    GACCCCAACC AGCCGGCCTC CGCCATCGTC AGCTTTGTCT CCAAGGTCAT GCTGAGTGCC
    GGCCAGAAGA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854877.1
    CDS343..5517
    Translation

    Target ORF information:

    RefSeq Version XM_024999109.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999109.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GGGGTGCAGG CTCGGGAGAA GCAGCCTGCA GAGCCCCCAG CACCTCTCCG GAAGCGGGCT
    GCCAGTGATG GGCAGTATGA GAACCAGTCA CCAGAACCCA CATCCCCCCG TAGCCCTGGG
    GTTCGCTCGC CTGTCCAATG TGTCTCCCCA GAGCTGGCTC TTACCATCGC TCTCAATCCT
    GGAGGGCGAC CCAAAGAGCC CCATCTGCAC AGCTACAAGG AAGCCTTTGA GGAGATGGAG
    GGAACCTCTC CGACCAGCCC ACCACCCAGT GGGGTGCGTT CCCCTCCAGG TCTGGCCAAG
    ACACCCTTGT CTGCTCTGGG CCTGAAGCCC CACAACCCAG CCGACATCCT GCTGCATCCC
    ACTGGTGTCC CCAGAAGACT GATCCAGCCA GAGCCCCGGA GCTACGTGGA GTCGGTGGTA
    CGAACGGCGG TGGCTGGGCC CCGGACTCAG GAACCTGAGC CCAAGAGCTT TAGCGCCCCA
    GGTGCGCAGG CGTATGGCCA TGAGACACCC CTGAGGATTG GGACCCTGGG AGGCTCCTTT
    GTCTCCCCTA GCCCCCTCTC CACCAGCAGC CCCATCCTCA GTGCTGACAG CACTTCAGTG
    GGGAGTTTCC CATCCGGGGA GAGCAGCGAC CAGGGCGCGC GAACACCTAC CCAGCCTCTG
    CTGGATTCTG GCTTCCGCTC CGGCAGCCTG GGCCAGCCAA GTCCTTTGGC TCAGAGAAAC
    TACCAGAGCT CTTCTCCTCT CCCAACTGCA GGCAGCAGCT ACAGCAGCCC CGACTACTCA
    CTGCAGCAGT TCAGCTCCCC AGAAGGCCAG GCCCGGTCTC AGTTCAGTGT GGCCGGTGTC
    CACACAGTGC CTGGGAGCCC CCAGGCACGG CACAGGACAG TGGGCACCAA TACACCCCCG
    AGCCCTGGCT TTGGCCGTCG GGCTGTGAAC CCCAGCCTGG CTGCCCCCAG CAGCCCCAGT
    TTGAGCCACC GCCAGGTGAT GGGTCCACTG GGAACCGGTT TCCATGGTAA CACGGGCTCC
    AGCCCCCAGA GCAGTGCAGC AACCACTCCG GGAAGCCCCA GTCTGGGTCG CCACCCTGGG
    GCCCACCAGG TTTCCAACCT CCATGGCAAT GTGGTCACCG CTCCTGGGAG CCCCAGCCTG
    GGCCGGCACC CCGGGGCCCA CCAAGGCACC CTGGCCTCCA ATCTCCATAG CAATGCAGTA
    GCCAGCCCCG GAAGCCCCAG CCTGGGCCGT CACTTGGGAG GGTCTGGATC TGTGGTTCCC
    GGCAGCCCCA GCTTGGACCG GCACGTGCCC TATGGTGGCT ACTCCACCCC TGAGGACCGG
    AGACCCACGC TGTCCCGGCA GAGCAGCGCC TCAGGCTACC AGGCTCCTTC CACGCCCTCC
    TTCCCTGTGT CCCCCGCCTA CTACCCTGGC CTGAGCAGCC CCGCCACCTC CCCGTCGCCT
    GATTCGGCAG CTTTCCGACA AGGGAGCCCC ACCCCGGCCT TGCCGGAGAA GCGGAGGATG
    TCCATGGGCG ACCGAGCGGG CAGCCTCCCC AACTACGCCA CCGTCAACGG GAAGGTGTCC
    TCCTCGCCGG TCGCCAGTGG CATGTCCAGT CCCAGCGGGG GCAGCACCGT CTCCTTCTCC
    CACACTCTGC CCGACTTCTC CAAGTACTCC ATGCCAGACA ACAGTCCTGA GACGCGGGCT
    AAAGTGAAGT TTGTCCAAGA CACTTCCAAG TATTGGTACA AGCCTGAGAT CTCCAGAGAA
    CAGGCCATCG CCCTCCTTAA GGACCAGGAG CCAGGGGCTT TCATCATCCG TGACAGCCAC
    TCCTTCCGAG GAGCCTATGG GCTGGCCATG AAGGTGTCTT CGCCCCCTCC GACCATCATG
    CAGCAGAATA AAAAAGGGGA CATGACCCAC GAGCTCGTGA GGCATTTCCT GATAGAGACC
    GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC CCCAATGAGC CAAACTTCGG ATCTCTGTCT
    GCCCTGGTCT ACCAGCACTC CATCATCCCG CTGGCCCTGC CCTGCAAGCT GGTCATTCCA
    AACCGAGACC CCACAGATGA ATCGAAAGAT AGCTCGGGCC CTGCCAACTC AACCAGTGAC
    CTGCTGAAGC AAGGGGCAGC CTGCAACGTG CTCTTCGTCA ACTCTGTGGA CATGGAGTCA
    CTCACTGGGC CACAGGCCAT CTCCAAAGCC ATTTCTGAGA CCCTGGCTGC TGACCCCACA
    CCAGCTGCCA CCATCGTTCA CTTCAAAGTT TCTGCCCAGG GAATTACACT GACTGACAAC
    CAGAGAAAGC TTTTCTTCAG GCGGCACTAC CCCCTCAACA CTGTGACCTT CTGTGACCTG
    GATCCACAGG AGAGGAAGTG GACAAAGACG GAGGGAGGTG CCCCTGCTAA GCTCTTCGGT
    TTTGTGGCCC GGAAACAGGG CAGCGCCACG GACAACGCCT GCCACCTCTT TGCTGAGCTT
    GACCCCAACC AGCCGGCCTC CGCCATCGTC AGCTTTGTCT CCAAGGTCAT GCTGAGTGCC
    GGCCAGAAGA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227174
    Clone ID Related Accession (Same CDS sequence) XM_024999109.1 , XM_024999158.1
    Accession Version XM_024999158.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GGGGTGCAGG CTCGGGAGAA GCAGCCTGCA GAGCCCCCAG CACCTCTCCG GAAGCGGGCT
    GCCAGTGATG GGCAGTATGA GAACCAGTCA CCAGAACCCA CATCCCCCCG TAGCCCTGGG
    GTTCGCTCGC CTGTCCAATG TGTCTCCCCA GAGCTGGCTC TTACCATCGC TCTCAATCCT
    GGAGGGCGAC CCAAAGAGCC CCATCTGCAC AGCTACAAGG AAGCCTTTGA GGAGATGGAG
    GGAACCTCTC CGACCAGCCC ACCACCCAGT GGGGTGCGTT CCCCTCCAGG TCTGGCCAAG
    ACACCCTTGT CTGCTCTGGG CCTGAAGCCC CACAACCCAG CCGACATCCT GCTGCATCCC
    ACTGGTGTCC CCAGAAGACT GATCCAGCCA GAGCCCCGGA GCTACGTGGA GTCGGTGGTA
    CGAACGGCGG TGGCTGGGCC CCGGACTCAG GAACCTGAGC CCAAGAGCTT TAGCGCCCCA
    GGTGCGCAGG CGTATGGCCA TGAGACACCC CTGAGGATTG GGACCCTGGG AGGCTCCTTT
    GTCTCCCCTA GCCCCCTCTC CACCAGCAGC CCCATCCTCA GTGCTGACAG CACTTCAGTG
    GGGAGTTTCC CATCCGGGGA GAGCAGCGAC CAGGGCGCGC GAACACCTAC CCAGCCTCTG
    CTGGATTCTG GCTTCCGCTC CGGCAGCCTG GGCCAGCCAA GTCCTTTGGC TCAGAGAAAC
    TACCAGAGCT CTTCTCCTCT CCCAACTGCA GGCAGCAGCT ACAGCAGCCC CGACTACTCA
    CTGCAGCAGT TCAGCTCCCC AGAAGGCCAG GCCCGGTCTC AGTTCAGTGT GGCCGGTGTC
    CACACAGTGC CTGGGAGCCC CCAGGCACGG CACAGGACAG TGGGCACCAA TACACCCCCG
    AGCCCTGGCT TTGGCCGTCG GGCTGTGAAC CCCAGCCTGG CTGCCCCCAG CAGCCCCAGT
    TTGAGCCACC GCCAGGTGAT GGGTCCACTG GGAACCGGTT TCCATGGTAA CACGGGCTCC
    AGCCCCCAGA GCAGTGCAGC AACCACTCCG GGAAGCCCCA GTCTGGGTCG CCACCCTGGG
    GCCCACCAGG TTTCCAACCT CCATGGCAAT GTGGTCACCG CTCCTGGGAG CCCCAGCCTG
    GGCCGGCACC CCGGGGCCCA CCAAGGCACC CTGGCCTCCA ATCTCCATAG CAATGCAGTA
    GCCAGCCCCG GAAGCCCCAG CCTGGGCCGT CACTTGGGAG GGTCTGGATC TGTGGTTCCC
    GGCAGCCCCA GCTTGGACCG GCACGTGCCC TATGGTGGCT ACTCCACCCC TGAGGACCGG
    AGACCCACGC TGTCCCGGCA GAGCAGCGCC TCAGGCTACC AGGCTCCTTC CACGCCCTCC
    TTCCCTGTGT CCCCCGCCTA CTACCCTGGC CTGAGCAGCC CCGCCACCTC CCCGTCGCCT
    GATTCGGCAG CTTTCCGACA AGGGAGCCCC ACCCCGGCCT TGCCGGAGAA GCGGAGGATG
    TCCATGGGCG ACCGAGCGGG CAGCCTCCCC AACTACGCCA CCGTCAACGG GAAGGTGTCC
    TCCTCGCCGG TCGCCAGTGG CATGTCCAGT CCCAGCGGGG GCAGCACCGT CTCCTTCTCC
    CACACTCTGC CCGACTTCTC CAAGTACTCC ATGCCAGACA ACAGTCCTGA GACGCGGGCT
    AAAGTGAAGT TTGTCCAAGA CACTTCCAAG TATTGGTACA AGCCTGAGAT CTCCAGAGAA
    CAGGCCATCG CCCTCCTTAA GGACCAGGAG CCAGGGGCTT TCATCATCCG TGACAGCCAC
    TCCTTCCGAG GAGCCTATGG GCTGGCCATG AAGGTGTCTT CGCCCCCTCC GACCATCATG
    CAGCAGAATA AAAAAGGGGA CATGACCCAC GAGCTCGTGA GGCATTTCCT GATAGAGACC
    GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC CCCAATGAGC CAAACTTCGG ATCTCTGTCT
    GCCCTGGTCT ACCAGCACTC CATCATCCCG CTGGCCCTGC CCTGCAAGCT GGTCATTCCA
    AACCGAGACC CCACAGATGA ATCGAAAGAT AGCTCGGGCC CTGCCAACTC AACCAGTGAC
    CTGCTGAAGC AAGGGGCAGC CTGCAACGTG CTCTTCGTCA ACTCTGTGGA CATGGAGTCA
    CTCACTGGGC CACAGGCCAT CTCCAAAGCC ATTTCTGAGA CCCTGGCTGC TGACCCCACA
    CCAGCTGCCA CCATCGTTCA CTTCAAAGTT TCTGCCCAGG GAATTACACT GACTGACAAC
    CAGAGAAAGC TTTTCTTCAG GCGGCACTAC CCCCTCAACA CTGTGACCTT CTGTGACCTG
    GATCCACAGG AGAGGAAGTG GACAAAGACG GAGGGAGGTG CCCCTGCTAA GCTCTTCGGT
    TTTGTGGCCC GGAAACAGGG CAGCGCCACG GACAACGCCT GCCACCTCTT TGCTGAGCTT
    GACCCCAACC AGCCGGCCTC CGCCATCGTC AGCTTTGTCT CCAAGGTCAT GCTGAGTGCC
    GGCCAGAAGA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854926.1
    CDS423..5597
    Translation

    Target ORF information:

    RefSeq Version XM_024999158.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999158.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GGGGTGCAGG CTCGGGAGAA GCAGCCTGCA GAGCCCCCAG CACCTCTCCG GAAGCGGGCT
    GCCAGTGATG GGCAGTATGA GAACCAGTCA CCAGAACCCA CATCCCCCCG TAGCCCTGGG
    GTTCGCTCGC CTGTCCAATG TGTCTCCCCA GAGCTGGCTC TTACCATCGC TCTCAATCCT
    GGAGGGCGAC CCAAAGAGCC CCATCTGCAC AGCTACAAGG AAGCCTTTGA GGAGATGGAG
    GGAACCTCTC CGACCAGCCC ACCACCCAGT GGGGTGCGTT CCCCTCCAGG TCTGGCCAAG
    ACACCCTTGT CTGCTCTGGG CCTGAAGCCC CACAACCCAG CCGACATCCT GCTGCATCCC
    ACTGGTGTCC CCAGAAGACT GATCCAGCCA GAGCCCCGGA GCTACGTGGA GTCGGTGGTA
    CGAACGGCGG TGGCTGGGCC CCGGACTCAG GAACCTGAGC CCAAGAGCTT TAGCGCCCCA
    GGTGCGCAGG CGTATGGCCA TGAGACACCC CTGAGGATTG GGACCCTGGG AGGCTCCTTT
    GTCTCCCCTA GCCCCCTCTC CACCAGCAGC CCCATCCTCA GTGCTGACAG CACTTCAGTG
    GGGAGTTTCC CATCCGGGGA GAGCAGCGAC CAGGGCGCGC GAACACCTAC CCAGCCTCTG
    CTGGATTCTG GCTTCCGCTC CGGCAGCCTG GGCCAGCCAA GTCCTTTGGC TCAGAGAAAC
    TACCAGAGCT CTTCTCCTCT CCCAACTGCA GGCAGCAGCT ACAGCAGCCC CGACTACTCA
    CTGCAGCAGT TCAGCTCCCC AGAAGGCCAG GCCCGGTCTC AGTTCAGTGT GGCCGGTGTC
    CACACAGTGC CTGGGAGCCC CCAGGCACGG CACAGGACAG TGGGCACCAA TACACCCCCG
    AGCCCTGGCT TTGGCCGTCG GGCTGTGAAC CCCAGCCTGG CTGCCCCCAG CAGCCCCAGT
    TTGAGCCACC GCCAGGTGAT GGGTCCACTG GGAACCGGTT TCCATGGTAA CACGGGCTCC
    AGCCCCCAGA GCAGTGCAGC AACCACTCCG GGAAGCCCCA GTCTGGGTCG CCACCCTGGG
    GCCCACCAGG TTTCCAACCT CCATGGCAAT GTGGTCACCG CTCCTGGGAG CCCCAGCCTG
    GGCCGGCACC CCGGGGCCCA CCAAGGCACC CTGGCCTCCA ATCTCCATAG CAATGCAGTA
    GCCAGCCCCG GAAGCCCCAG CCTGGGCCGT CACTTGGGAG GGTCTGGATC TGTGGTTCCC
    GGCAGCCCCA GCTTGGACCG GCACGTGCCC TATGGTGGCT ACTCCACCCC TGAGGACCGG
    AGACCCACGC TGTCCCGGCA GAGCAGCGCC TCAGGCTACC AGGCTCCTTC CACGCCCTCC
    TTCCCTGTGT CCCCCGCCTA CTACCCTGGC CTGAGCAGCC CCGCCACCTC CCCGTCGCCT
    GATTCGGCAG CTTTCCGACA AGGGAGCCCC ACCCCGGCCT TGCCGGAGAA GCGGAGGATG
    TCCATGGGCG ACCGAGCGGG CAGCCTCCCC AACTACGCCA CCGTCAACGG GAAGGTGTCC
    TCCTCGCCGG TCGCCAGTGG CATGTCCAGT CCCAGCGGGG GCAGCACCGT CTCCTTCTCC
    CACACTCTGC CCGACTTCTC CAAGTACTCC ATGCCAGACA ACAGTCCTGA GACGCGGGCT
    AAAGTGAAGT TTGTCCAAGA CACTTCCAAG TATTGGTACA AGCCTGAGAT CTCCAGAGAA
    CAGGCCATCG CCCTCCTTAA GGACCAGGAG CCAGGGGCTT TCATCATCCG TGACAGCCAC
    TCCTTCCGAG GAGCCTATGG GCTGGCCATG AAGGTGTCTT CGCCCCCTCC GACCATCATG
    CAGCAGAATA AAAAAGGGGA CATGACCCAC GAGCTCGTGA GGCATTTCCT GATAGAGACC
    GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC CCCAATGAGC CAAACTTCGG ATCTCTGTCT
    GCCCTGGTCT ACCAGCACTC CATCATCCCG CTGGCCCTGC CCTGCAAGCT GGTCATTCCA
    AACCGAGACC CCACAGATGA ATCGAAAGAT AGCTCGGGCC CTGCCAACTC AACCAGTGAC
    CTGCTGAAGC AAGGGGCAGC CTGCAACGTG CTCTTCGTCA ACTCTGTGGA CATGGAGTCA
    CTCACTGGGC CACAGGCCAT CTCCAAAGCC ATTTCTGAGA CCCTGGCTGC TGACCCCACA
    CCAGCTGCCA CCATCGTTCA CTTCAAAGTT TCTGCCCAGG GAATTACACT GACTGACAAC
    CAGAGAAAGC TTTTCTTCAG GCGGCACTAC CCCCTCAACA CTGTGACCTT CTGTGACCTG
    GATCCACAGG AGAGGAAGTG GACAAAGACG GAGGGAGGTG CCCCTGCTAA GCTCTTCGGT
    TTTGTGGCCC GGAAACAGGG CAGCGCCACG GACAACGCCT GCCACCTCTT TGCTGAGCTT
    GACCCCAACC AGCCGGCCTC CGCCATCGTC AGCTTTGTCT CCAAGGTCAT GCTGAGTGCC
    GGCCAGAAGA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227175
    Clone ID Related Accession (Same CDS sequence) XM_024999116.1
    Accession Version XM_024999116.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGACATGGA TCTGTCTATC CTGCATGCTC TGGCCAGAGG ACCTGGAGGC CCCCAAGACT 
    CACCACTTCA AGGTGAAGAC CTTCAAGAAG GTGAAGCCCT GTGGGATCTG CCGACAGGTC
    ATCACCCGAG AGGGCTGCAC CTGCAAAGTC TGCAGCTTCT CCTGTCATCG GAAATGCCAG
    GCCAAGGTGG CTGCCCCCTG CATTCCTCCT TCCAGCCATG AACTGGTGCC CGTCAACACT
    GAGACTGCTC CGAAGAATGT AGTGGACACG GGAGAAGCAG CCTCTCGGGG TGGAAACACA
    CGGAAAAGCC TTGAGGAAGA TAGTTCCACC AGAGTTACAC CAAGTGTCCA GCCTCATCCC
    CAGCCCATCA GAAACATGAG TACGAGCCGG ACCACGGAGG ACAGCTGTGA GCTGGACCTG
    GTGTATGTCA CCGAGAGGAT CATCGCCGTC TCCTTCCCGA GCACAGCCAA TGAGGAGAAC
    TTCCGGAGCA ACCTCCGAGA GGTGGCCCAG ATGCTCAAGT CCAAACATGG AGGCAACTAC
    CTGCTTTTCA ACCTCTCTGA GCGGAGACCT GACATCACGA AGCTCCACGC CAAGGTCCTG
    GAATTTGGCT GGCCCGACCT CCACACCCCG GCCCTGGAGA AGATCTGCAG CGTCTGCAAG
    GCCATGGACA CCTGGCTCAA TGCAGACCCC CACAACGTGG TCGTGCTACA CAACAAGGGA
    AACCGAGGCA GGATCGGCGT TGTGATCGCG GCTTACATGC ACTACAGCAA CATTTCTGCC
    AGTGCCGACC AGGCCCTGGA CCGGTTTGCG ATGAAACGGT TCTACGAGGA TAAGATTGTA
    CCCATTGGCC AACCATCCCA GAGGAGGTAC GTGCATTATT TCAGCGGCCT GCTCTCTGGC
    TCCATCAAAA TGAACAACAA GCCCTTGTTT CTGCACCACG TGATCATGCA CGGCATCCCC
    AACTTCGAGT CTAAAGGAGG GTGTCGACCA TTTCTCCGGA TCTACCAGGC CATGCAACCT
    GTCTACACAT CTGGCATCTA CAACGTCCAA GGAGACAGCC AGACCAGCAT CTGCATCACC
    ATTGAGCCTG GGCTGCTCTT GAAGGGAGAC ATCTTGCTGA AGTGCTACCA CAAGAAGTTC
    CGGAGCCCAG CCCGAGATGT CATCTTCAGG GTGCAGTTCC ACACCTGTGC CATCCATGAC
    CTGGGAGTTG TCTTTGGGAA GGAGGACCTT GACGATGCCT TCAAGGACGA TCGATTTCCA
    GAATACGGCA AAGTTGAATT TGTATTTTCT TATGGACCAG AGAAAATTCA AGGCATGGAA
    CACCTGGAGA ATGGCCCGAG CGTGTCCGTG GACTATAACA CCTCTGACCC CCTCATCCGC
    TGGGATTCCT ATGACAACTT CAATGGGCAT CGAGATGATG GCATGGAGGA GGTTGTGGGC
    CATACCCAGG GACCCCTGGA CGGGAGCCTG TATGCCAAGG TGAAGAAGAA GGACTCCTTG
    CACGGCAGCA CTGGGGCTGT CAACGCCACG CGGCCCGTGC TGTCAGCCAC CCCCAATCAC
    GTGGAGCACA CCCTCTCCGT GAGCAGTGAC TCGGGCAACT CCACAGCCTC CACCAAGACA
    GACAAGACCG ATGAGCCTGC CCCCGGCCCT GCCAGTGCCC CTGCTGCCCT GAGTCCCGAG
    GAGAAGCGTG AGCTGGACCG CCTGCTCAGT GGCTTTGGCT TGGAGCGGGA GAAGCAAGGC
    GCCATGTACC ACCCTCAGCA TCTCCGGTCC CGCCCAGTGG GAGGCCCTGC TGCCCCTTCC
    TCTGGCCGCC ACATCGTCCC GGCCCAGGTT CACGTCAACG GTGGGGCCTT AGCGTCCGAG
    CGGGAGACAG ACATCCTGGA TGATGAGCTG CCCAACCAGG ATGGGCATAG CGTGGGCAGC
    ATGGGCACCC TGTCCTCCCT GGATGGAGTC ACCAACACAA GTGAGGGTGG CTACCCGGAG
    GCCCTCTCCC CGCTGACCAA CGGTCTGGAC AAGCCCTACC CCATGGAACC TATGGTCAAC
    GGTGGGGGCT ACCCCTATGA GTCTGCCAGC CGGGCAGTCT CTGCCCAGGC TGGCCACACA
    GCCCCCATGA GACCCTCCTA CTCCACACAG GAGGGTTTAG CTGGCTACCA GCGGGAGGGA
    CCCCACCCAG CCTGGCCACA GTCAGCGACC ACCTCCCACT ATGGCCATGA CCCCAACGGT
    ATGTTTCGCT CTCAATCCTT TCCTGAAACG GAGCCCCAGC TGCCCCCAGC TCCAGCCCGT
    GGTGGGAGCA GCCGGGAGGC TGTGCAGAGG GGTCTGAATT CCTGGCAGCA GCAGCAGCAA
    CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCTCGCC AACAGGAAAG AGTCCACTTG
    GAGAGCCTTG GGCTCAGCAG GCCCAGCCCC CAGCCACTAG CAGAGCCCCC CATGTCTGGC
    CTCCCTGAGT TCCCTCGCGC AGCCTCCCAG CAGGAGATCG AGCAGTCCAT CGAAGCCCTC
    AATATGCTGA TGCTCGATTT GGAACCAGCC ACGGCGGGCG CCCCCCTACA CAAGTCCCAG
    AGCGTGCCTG GGGCCTGGCC AGGGGCTTCT CCGCTCTCTT CCCAGCCCTT CTCTGGCTCC
    TCTTGCCAGT CCCACCCACT GACCCAGTCC AGATCTGGCT ACATCCCCAG TGGGCATTCC
    TTGGGAACCC CTGAGCCAGC CCCCCGGGCC CCCCTGGAGT CTGTTCCCAC TGGCAGGCCT
    TACTCACCTT ATGATTATCA GCCATGTCCA ACTGGGCCCA ACCAGAGTTA CCATCCAAAG
    AGCCCGGCCA CCTCCTCCTC GTCTTCCTTC CTTCCAACCA CCCAGAGCTC TGTGGGGCCT
    CAGCAGCCCC CAGCCTCTCT CCCTGGCCTC ACCACTCAGC CTCAGCTTCC ACCAAAGGAG
    GTGACTTCAG ACCCATCCCG AACTCCAGAG GAGGAACCAT TGAACTTGGA AGGGCTGGTG
    GCCCACCGGG TGGCAGGGGT GCAGGCTCGG GAGAAGCAGC CTGCAGAGCC CCCAGCACCT
    CTCCGGAAGC GGGCTGCCAG TGATGGGCAG TATGAGAACC AGTCACCAGA ACCCACATCC
    CCCCGTAGCC CTGGGGTTCG CTCGCCTGTC CAATGTGTCT CCCCAGAGCT GGCTCTTACC
    ATCGCTCTCA ATCCTGGAGG GCGACCCAAA GAGCCCCATC TGCACAGCTA CAAGGAAGCC
    TTTGAGGAGA TGGAGGGAAC CTCTCCGACC AGCCCACCAC CCAGTGGGGT GCGTTCCCCT
    CCAGGTCTGG CCAAGACACC CTTGTCTGCT CTGGGCCTGA AGCCCCACAA CCCAGCCGAC
    ATCCTGCTGC ATCCCACTGG TGAGCCCCGG AGCTACGTGG AGTCGGTGGT ACGAACGGCG
    GTGGCTGGGC CCCGGACTCA GGAACCTGAG CCCAAGAGCT TTAGCGCCCC AGGTGCGCAG
    GCGTATGGCC ATGAGACACC CCTGAGGATT GGGACCCTGG GAGGCTCCTT TGTCTCCCCT
    AGCCCCCTCT CCACCAGCAG CCCCATCCTC AGTGCTGACA GCACTTCAGT GGGGAGTTTC
    CCATCCGGGG AGAGCAGCGA CCAGGGCGCG CGAACACCTA CCCAGCCTCT GCTGGATTCT
    GGCTTCCGCT CCGGCAGCCT GGGCCAGCCA AGTCCTTTGG CTCAGAGAAA CTACCAGAGC
    TCTTCTCCTC TCCCAACTGC AGGCAGCAGC TACAGCAGCC CCGACTACTC ACTGCAGCAG
    TTCAGCTCCC CAGAAGGCCA GGCCCGGTCT CAGTTCAGTG TGGCCGGTGT CCACACAGTG
    CCTGGGAGCC CCCAGGCACG GCACAGGACA GTGGGCACCA ATACACCCCC GAGCCCTGGC
    TTTGGCCGTC GGGCTGTGAA CCCCAGCCTG GCTGCCCCCA GCAGCCCCAG TTTGAGCCAC
    CGCCAGGTGA TGGGTCCACT GGGAACCGGT TTCCATGGTA ACACGGGCTC CAGCCCCCAG
    AGCAGTGCAG CAACCACTCC GGGAAGCCCC AGTCTGGGTC GCCACCCTGG GGCCCACCAG
    GTTTCCAACC TCCATGGCAA TGTGGTCACC GCTCCTGGGA GCCCCAGCCT GGGCCGGCAC
    CCCGGGGCCC ACCAAGGCAC CCTGGCCTCC AATCTCCATA GCAATGCAGT AGCCAGCCCC
    GGAAGCCCCA GCCTGGGCCG TCACTTGGGA GGGTCTGGAT CTGTGGTTCC CGGCAGCCCC
    AGCTTGGACC GGCACGTGCC CTATGGTGGC TACTCCACCC CTGAGGACCG GAGACCCACG
    CTGTCCCGGC AGAGCAGCGC CTCAGGCTAC CAGGCTCCTT CCACGCCCTC CTTCCCTGTG
    TCCCCCGCCT ACTACCCTGG CCTGAGCAGC CCCGCCACCT CCCCGTCGCC TGATTCGGCA
    GCTTTCCGAC AAGGGAGCCC CACCCCGGCC TTGCCGGAGA AGCGGAGGAT GTCCATGGGC
    GACCGAGCGG GCAGCCTCCC CAACTACGCC ACCGTCAACG GGAAGGTGTC CTCCTCGCCG
    GTCGCCAGTG GCATGTCCAG TCCCAGCGGG GGCAGCACCG TCTCCTTCTC CCACACTCTG
    CCCGACTTCT CCAAGTACTC CATGCCAGAC AACAGTCCTG AGACGCGGGC TAAAGTGAAG
    TTTGTCCAAG ACACTTCCAA GTATTGGTAC AAGCCTGAGA TCTCCAGAGA ACAGGCCATC
    GCCCTCCTTA AGGACCAGGA GCCAGGGGCT TTCATCATCC GTGACAGCCA CTCCTTCCGA
    GGAGCCTATG GGCTGGCCAT GAAGGTGTCT TCGCCCCCTC CGACCATCAT GCAGCAGAAT
    AAAAAAGGGG ACATGACCCA CGAGCTCGTG AGGCATTTCC TGATAGAGAC CGGCCCCAGA
    GGAGTCAAGC TCAAGGGCTG CCCCAATGAG CCAAACTTCG GATCTCTGTC TGCCCTGGTC
    TACCAGCACT CCATCATCCC GCTGGCCCTG CCCTGCAAGC TGGTCATTCC AAACCGAGAC
    CCCACAGATG AATCGAAAGA TAGCTCGGGC CCTGCCAACT CAACCAGTGA CCTGCTGAAG
    CAAGGGGCAG CCTGCAACGT GCTCTTCGTC AACTCTGTGG ACATGGAGTC ACTCACTGGG
    CCACAGGCCA TCTCCAAAGC CATTTCTGAG ACCCTGGCTG CTGACCCCAC ACCAGCTGCC
    ACCATCGTTC ACTTCAAAGT TTCTGCCCAG GGAATTACAC TGACTGACAA CCAGAGAAAG
    CTTTTCTTCA GGCGGCACTA CCCCCTCAAC ACTGTGACCT TCTGTGACCT GGATCCACAG
    GAGAGGAAGT GGACAAAGAC GGAGGGAGGT GCCCCTGCTA AGCTCTTCGG TTTTGTGGCC
    CGGAAACAGG GCAGCGCCAC GGACAACGCC TGCCACCTCT TTGCTGAGCT TGACCCCAAC
    CAGCCGGCCT CCGCCATCGT CAGCTTTGTC TCCAAGGTCA TGCTGAGTGC CGGCCAGAAG
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854884.1
    CDS120..5645
    Translation

    Target ORF information:

    RefSeq Version XM_024999116.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999116.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGACATGGA TCTGTCTATC CTGCATGCTC TGGCCAGAGG ACCTGGAGGC CCCCAAGACT 
    CACCACTTCA AGGTGAAGAC CTTCAAGAAG GTGAAGCCCT GTGGGATCTG CCGACAGGTC
    ATCACCCGAG AGGGCTGCAC CTGCAAAGTC TGCAGCTTCT CCTGTCATCG GAAATGCCAG
    GCCAAGGTGG CTGCCCCCTG CATTCCTCCT TCCAGCCATG AACTGGTGCC CGTCAACACT
    GAGACTGCTC CGAAGAATGT AGTGGACACG GGAGAAGCAG CCTCTCGGGG TGGAAACACA
    CGGAAAAGCC TTGAGGAAGA TAGTTCCACC AGAGTTACAC CAAGTGTCCA GCCTCATCCC
    CAGCCCATCA GAAACATGAG TACGAGCCGG ACCACGGAGG ACAGCTGTGA GCTGGACCTG
    GTGTATGTCA CCGAGAGGAT CATCGCCGTC TCCTTCCCGA GCACAGCCAA TGAGGAGAAC
    TTCCGGAGCA ACCTCCGAGA GGTGGCCCAG ATGCTCAAGT CCAAACATGG AGGCAACTAC
    CTGCTTTTCA ACCTCTCTGA GCGGAGACCT GACATCACGA AGCTCCACGC CAAGGTCCTG
    GAATTTGGCT GGCCCGACCT CCACACCCCG GCCCTGGAGA AGATCTGCAG CGTCTGCAAG
    GCCATGGACA CCTGGCTCAA TGCAGACCCC CACAACGTGG TCGTGCTACA CAACAAGGGA
    AACCGAGGCA GGATCGGCGT TGTGATCGCG GCTTACATGC ACTACAGCAA CATTTCTGCC
    AGTGCCGACC AGGCCCTGGA CCGGTTTGCG ATGAAACGGT TCTACGAGGA TAAGATTGTA
    CCCATTGGCC AACCATCCCA GAGGAGGTAC GTGCATTATT TCAGCGGCCT GCTCTCTGGC
    TCCATCAAAA TGAACAACAA GCCCTTGTTT CTGCACCACG TGATCATGCA CGGCATCCCC
    AACTTCGAGT CTAAAGGAGG GTGTCGACCA TTTCTCCGGA TCTACCAGGC CATGCAACCT
    GTCTACACAT CTGGCATCTA CAACGTCCAA GGAGACAGCC AGACCAGCAT CTGCATCACC
    ATTGAGCCTG GGCTGCTCTT GAAGGGAGAC ATCTTGCTGA AGTGCTACCA CAAGAAGTTC
    CGGAGCCCAG CCCGAGATGT CATCTTCAGG GTGCAGTTCC ACACCTGTGC CATCCATGAC
    CTGGGAGTTG TCTTTGGGAA GGAGGACCTT GACGATGCCT TCAAGGACGA TCGATTTCCA
    GAATACGGCA AAGTTGAATT TGTATTTTCT TATGGACCAG AGAAAATTCA AGGCATGGAA
    CACCTGGAGA ATGGCCCGAG CGTGTCCGTG GACTATAACA CCTCTGACCC CCTCATCCGC
    TGGGATTCCT ATGACAACTT CAATGGGCAT CGAGATGATG GCATGGAGGA GGTTGTGGGC
    CATACCCAGG GACCCCTGGA CGGGAGCCTG TATGCCAAGG TGAAGAAGAA GGACTCCTTG
    CACGGCAGCA CTGGGGCTGT CAACGCCACG CGGCCCGTGC TGTCAGCCAC CCCCAATCAC
    GTGGAGCACA CCCTCTCCGT GAGCAGTGAC TCGGGCAACT CCACAGCCTC CACCAAGACA
    GACAAGACCG ATGAGCCTGC CCCCGGCCCT GCCAGTGCCC CTGCTGCCCT GAGTCCCGAG
    GAGAAGCGTG AGCTGGACCG CCTGCTCAGT GGCTTTGGCT TGGAGCGGGA GAAGCAAGGC
    GCCATGTACC ACCCTCAGCA TCTCCGGTCC CGCCCAGTGG GAGGCCCTGC TGCCCCTTCC
    TCTGGCCGCC ACATCGTCCC GGCCCAGGTT CACGTCAACG GTGGGGCCTT AGCGTCCGAG
    CGGGAGACAG ACATCCTGGA TGATGAGCTG CCCAACCAGG ATGGGCATAG CGTGGGCAGC
    ATGGGCACCC TGTCCTCCCT GGATGGAGTC ACCAACACAA GTGAGGGTGG CTACCCGGAG
    GCCCTCTCCC CGCTGACCAA CGGTCTGGAC AAGCCCTACC CCATGGAACC TATGGTCAAC
    GGTGGGGGCT ACCCCTATGA GTCTGCCAGC CGGGCAGTCT CTGCCCAGGC TGGCCACACA
    GCCCCCATGA GACCCTCCTA CTCCACACAG GAGGGTTTAG CTGGCTACCA GCGGGAGGGA
    CCCCACCCAG CCTGGCCACA GTCAGCGACC ACCTCCCACT ATGGCCATGA CCCCAACGGT
    ATGTTTCGCT CTCAATCCTT TCCTGAAACG GAGCCCCAGC TGCCCCCAGC TCCAGCCCGT
    GGTGGGAGCA GCCGGGAGGC TGTGCAGAGG GGTCTGAATT CCTGGCAGCA GCAGCAGCAA
    CAGCAGCAGC AGCAGCAGCA GCCTCGCCCA CCTCCTCGCC AACAGGAAAG AGTCCACTTG
    GAGAGCCTTG GGCTCAGCAG GCCCAGCCCC CAGCCACTAG CAGAGCCCCC CATGTCTGGC
    CTCCCTGAGT TCCCTCGCGC AGCCTCCCAG CAGGAGATCG AGCAGTCCAT CGAAGCCCTC
    AATATGCTGA TGCTCGATTT GGAACCAGCC ACGGCGGGCG CCCCCCTACA CAAGTCCCAG
    AGCGTGCCTG GGGCCTGGCC AGGGGCTTCT CCGCTCTCTT CCCAGCCCTT CTCTGGCTCC
    TCTTGCCAGT CCCACCCACT GACCCAGTCC AGATCTGGCT ACATCCCCAG TGGGCATTCC
    TTGGGAACCC CTGAGCCAGC CCCCCGGGCC CCCCTGGAGT CTGTTCCCAC TGGCAGGCCT
    TACTCACCTT ATGATTATCA GCCATGTCCA ACTGGGCCCA ACCAGAGTTA CCATCCAAAG
    AGCCCGGCCA CCTCCTCCTC GTCTTCCTTC CTTCCAACCA CCCAGAGCTC TGTGGGGCCT
    CAGCAGCCCC CAGCCTCTCT CCCTGGCCTC ACCACTCAGC CTCAGCTTCC ACCAAAGGAG
    GTGACTTCAG ACCCATCCCG AACTCCAGAG GAGGAACCAT TGAACTTGGA AGGGCTGGTG
    GCCCACCGGG TGGCAGGGGT GCAGGCTCGG GAGAAGCAGC CTGCAGAGCC CCCAGCACCT
    CTCCGGAAGC GGGCTGCCAG TGATGGGCAG TATGAGAACC AGTCACCAGA ACCCACATCC
    CCCCGTAGCC CTGGGGTTCG CTCGCCTGTC CAATGTGTCT CCCCAGAGCT GGCTCTTACC
    ATCGCTCTCA ATCCTGGAGG GCGACCCAAA GAGCCCCATC TGCACAGCTA CAAGGAAGCC
    TTTGAGGAGA TGGAGGGAAC CTCTCCGACC AGCCCACCAC CCAGTGGGGT GCGTTCCCCT
    CCAGGTCTGG CCAAGACACC CTTGTCTGCT CTGGGCCTGA AGCCCCACAA CCCAGCCGAC
    ATCCTGCTGC ATCCCACTGG TGAGCCCCGG AGCTACGTGG AGTCGGTGGT ACGAACGGCG
    GTGGCTGGGC CCCGGACTCA GGAACCTGAG CCCAAGAGCT TTAGCGCCCC AGGTGCGCAG
    GCGTATGGCC ATGAGACACC CCTGAGGATT GGGACCCTGG GAGGCTCCTT TGTCTCCCCT
    AGCCCCCTCT CCACCAGCAG CCCCATCCTC AGTGCTGACA GCACTTCAGT GGGGAGTTTC
    CCATCCGGGG AGAGCAGCGA CCAGGGCGCG CGAACACCTA CCCAGCCTCT GCTGGATTCT
    GGCTTCCGCT CCGGCAGCCT GGGCCAGCCA AGTCCTTTGG CTCAGAGAAA CTACCAGAGC
    TCTTCTCCTC TCCCAACTGC AGGCAGCAGC TACAGCAGCC CCGACTACTC ACTGCAGCAG
    TTCAGCTCCC CAGAAGGCCA GGCCCGGTCT CAGTTCAGTG TGGCCGGTGT CCACACAGTG
    CCTGGGAGCC CCCAGGCACG GCACAGGACA GTGGGCACCA ATACACCCCC GAGCCCTGGC
    TTTGGCCGTC GGGCTGTGAA CCCCAGCCTG GCTGCCCCCA GCAGCCCCAG TTTGAGCCAC
    CGCCAGGTGA TGGGTCCACT GGGAACCGGT TTCCATGGTA ACACGGGCTC CAGCCCCCAG
    AGCAGTGCAG CAACCACTCC GGGAAGCCCC AGTCTGGGTC GCCACCCTGG GGCCCACCAG
    GTTTCCAACC TCCATGGCAA TGTGGTCACC GCTCCTGGGA GCCCCAGCCT GGGCCGGCAC
    CCCGGGGCCC ACCAAGGCAC CCTGGCCTCC AATCTCCATA GCAATGCAGT AGCCAGCCCC
    GGAAGCCCCA GCCTGGGCCG TCACTTGGGA GGGTCTGGAT CTGTGGTTCC CGGCAGCCCC
    AGCTTGGACC GGCACGTGCC CTATGGTGGC TACTCCACCC CTGAGGACCG GAGACCCACG
    CTGTCCCGGC AGAGCAGCGC CTCAGGCTAC CAGGCTCCTT CCACGCCCTC CTTCCCTGTG
    TCCCCCGCCT ACTACCCTGG CCTGAGCAGC CCCGCCACCT CCCCGTCGCC TGATTCGGCA
    GCTTTCCGAC AAGGGAGCCC CACCCCGGCC TTGCCGGAGA AGCGGAGGAT GTCCATGGGC
    GACCGAGCGG GCAGCCTCCC CAACTACGCC ACCGTCAACG GGAAGGTGTC CTCCTCGCCG
    GTCGCCAGTG GCATGTCCAG TCCCAGCGGG GGCAGCACCG TCTCCTTCTC CCACACTCTG
    CCCGACTTCT CCAAGTACTC CATGCCAGAC AACAGTCCTG AGACGCGGGC TAAAGTGAAG
    TTTGTCCAAG ACACTTCCAA GTATTGGTAC AAGCCTGAGA TCTCCAGAGA ACAGGCCATC
    GCCCTCCTTA AGGACCAGGA GCCAGGGGCT TTCATCATCC GTGACAGCCA CTCCTTCCGA
    GGAGCCTATG GGCTGGCCAT GAAGGTGTCT TCGCCCCCTC CGACCATCAT GCAGCAGAAT
    AAAAAAGGGG ACATGACCCA CGAGCTCGTG AGGCATTTCC TGATAGAGAC CGGCCCCAGA
    GGAGTCAAGC TCAAGGGCTG CCCCAATGAG CCAAACTTCG GATCTCTGTC TGCCCTGGTC
    TACCAGCACT CCATCATCCC GCTGGCCCTG CCCTGCAAGC TGGTCATTCC AAACCGAGAC
    CCCACAGATG AATCGAAAGA TAGCTCGGGC CCTGCCAACT CAACCAGTGA CCTGCTGAAG
    CAAGGGGCAG CCTGCAACGT GCTCTTCGTC AACTCTGTGG ACATGGAGTC ACTCACTGGG
    CCACAGGCCA TCTCCAAAGC CATTTCTGAG ACCCTGGCTG CTGACCCCAC ACCAGCTGCC
    ACCATCGTTC ACTTCAAAGT TTCTGCCCAG GGAATTACAC TGACTGACAA CCAGAGAAAG
    CTTTTCTTCA GGCGGCACTA CCCCCTCAAC ACTGTGACCT TCTGTGACCT GGATCCACAG
    GAGAGGAAGT GGACAAAGAC GGAGGGAGGT GCCCCTGCTA AGCTCTTCGG TTTTGTGGCC
    CGGAAACAGG GCAGCGCCAC GGACAACGCC TGCCACCTCT TTGCTGAGCT TGACCCCAAC
    CAGCCGGCCT CCGCCATCGT CAGCTTTGTC TCCAAGGTCA TGCTGAGTGC CGGCCAGAAG
    AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227176
    Clone ID Related Accession (Same CDS sequence) XM_024999120.1
    Accession Version XM_024999120.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAATCCTG CTGGTCGTCA GGACTTGGGG AGTTCCACCA GAGTTACACC AAGTGTCCAG 
    CCTCATCCCC AGCCCATCAG AAACATGAGT ACGAGCCGGA CCACGGAGGA CAGCTGTGAG
    CTGGACCTGG TGTATGTCAC CGAGAGGATC ATCGCCGTCT CCTTCCCGAG CACAGCCAAT
    GAGGAGAACT TCCGGAGCAA CCTCCGAGAG GTGGCCCAGA TGCTCAAGTC CAAACATGGA
    GGCAACTACC TGCTTTTCAA CCTCTCTGAG CGGAGACCTG ACATCACGAA GCTCCACGCC
    AAGGTCCTGG AATTTGGCTG GCCCGACCTC CACACCCCGG CCCTGGAGAA GATCTGCAGC
    GTCTGCAAGG CCATGGACAC CTGGCTCAAT GCAGACCCCC ACAACGTGGT CGTGCTACAC
    AACAAGGGAA ACCGAGGCAG GATCGGCGTT GTGATCGCGG CTTACATGCA CTACAGCAAC
    ATTTCTGCCA GTGCCGACCA GGCCCTGGAC CGGTTTGCGA TGAAACGGTT CTACGAGGAT
    AAGATTGTAC CCATTGGCCA ACCATCCCAG AGGAGGTACG TGCATTATTT CAGCGGCCTG
    CTCTCTGGCT CCATCAAAAT GAACAACAAG CCCTTGTTTC TGCACCACGT GATCATGCAC
    GGCATCCCCA ACTTCGAGTC TAAAGGAGGG TGTCGACCAT TTCTCCGGAT CTACCAGGCC
    ATGCAACCTG TCTACACATC TGGCATCTAC AACGTCCAAG GAGACAGCCA GACCAGCATC
    TGCATCACCA TTGAGCCTGG GCTGCTCTTG AAGGGAGACA TCTTGCTGAA GTGCTACCAC
    AAGAAGTTCC GGAGCCCAGC CCGAGATGTC ATCTTCAGGG TGCAGTTCCA CACCTGTGCC
    ATCCATGACC TGGGAGTTGT CTTTGGGAAG GAGGACCTTG ACGATGCCTT CAAGGACGAT
    CGATTTCCAG AATACGGCAA AGTTGAATTT GTATTTTCTT ATGGACCAGA GAAAATTCAA
    GGCATGGAAC ACCTGGAGAA TGGCCCGAGC GTGTCCGTGG ACTATAACAC CTCTGACCCC
    CTCATCCGCT GGGATTCCTA TGACAACTTC AATGGGCATC GAGATGATGG CATGGAGGAG
    GTTGTGGGCC ATACCCAGGG ACCCCTGGAC GGGAGCCTGT ATGCCAAGGT GAAGAAGAAG
    GACTCCTTGC ACGGCAGCAC TGGGGCTGTC AACGCCACGC GGCCCGTGCT GTCAGCCACC
    CCCAATCACG TGGAGCACAC CCTCTCCGTG AGCAGTGACT CGGGCAACTC CACAGCCTCC
    ACCAAGACAG ACAAGACCGA TGAGCCTGCC CCCGGCCCTG CCAGTGCCCC TGCTGCCCTG
    AGTCCCGAGG AGAAGCGTGA GCTGGACCGC CTGCTCAGTG GCTTTGGCTT GGAGCGGGAG
    AAGCAAGGCG CCATGTACCA CCCTCAGCAT CTCCGGTCCC GCCCAGTGGG AGGCCCTGCT
    GCCCCTTCCT CTGGCCGCCA CATCGTCCCG GCCCAGGTTC ACGTCAACGG TGGGGCCTTA
    GCGTCCGAGC GGGAGACAGA CATCCTGGAT GATGAGCTGC CCAACCAGGA TGGGCATAGC
    GTGGGCAGCA TGGGCACCCT GTCCTCCCTG GATGGAGTCA CCAACACAAG TGAGGGTGGC
    TACCCGGAGG CCCTCTCCCC GCTGACCAAC GGTCTGGACA AGCCCTACCC CATGGAACCT
    ATGGTCAACG GTGGGGGCTA CCCCTATGAG TCTGCCAGCC GGGCAGTCTC TGCCCAGGCT
    GGCCACACAG CCCCCATGAG ACCCTCCTAC TCCACACAGG AGGGTTTAGC TGGCTACCAG
    CGGGAGGGAC CCCACCCAGC CTGGCCACAG TCAGCGACCA CCTCCCACTA TGGCCATGAC
    CCCAACGGTA TGTTTCGCTC TCAATCCTTT CCTGAAACGG AGCCCCAGCT GCCCCCAGCT
    CCAGCCCGTG GTGGGAGCAG CCGGGAGGCT GTGCAGAGGG GTCTGAATTC CTGGCAGCAG
    CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG CCTCGCCCAC CTCCTCGCCA ACAGGAAAGA
    GTCCACTTGG AGAGCCTTGG GCTCAGCAGG CCCAGCCCCC AGCCACTAGC AGAGCCCCCC
    ATGTCTGGCC TCCCTGAGTT CCCTCGCGCA GCCTCCCAGC AGGAGATCGA GCAGTCCATC
    GAAGCCCTCA ATATGCTGAT GCTCGATTTG GAACCAGCCA CGGCGGGCGC CCCCCTACAC
    AAGTCCCAGA GCGTGCCTGG GGCCTGGCCA GGGGCTTCTC CGCTCTCTTC CCAGCCCTTC
    TCTGGCTCCT CTTGCCAGTC CCACCCACTG ACCCAGTCCA GATCTGGCTA CATCCCCAGT
    GGGCATTCCT TGGGAACCCC TGAGCCAGCC CCCCGGGCCC CCCTGGAGTC TGTTCCCACT
    GGCAGGCCTT ACTCACCTTA TGATTATCAG CCATGTCCAA CTGGGCCCAA CCAGAGTTAC
    CATCCAAAGA GCCCGGCCAC CTCCTCCTCG TCTTCCTTCC TTCCAACCAC CCAGAGCTCT
    GTGGGGCCTC AGCAGCCCCC AGCCTCTCTC CCTGGCCTCA CCACTCAGCC TCAGCTTCCA
    CCAAAGGAGG TGACTTCAGA CCCATCCCGA ACTCCAGAGG AGGAACCATT GAACTTGGAA
    GGGCTGGTGG CCCACCGGGT GGCAGCGTAC AATGCCAGGC TGCAGGGCAC TGGGCGGAGA
    GTCAGCTCCT GGCACCCTCC TCTCCACCGG CTCCGATCTT CCTCTCTCTC TGGGGTGCAG
    GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCACCTCTCC GGAAGCGGGC TGCCAGTGAT
    GGGCAGTATG AGAACCAGTC ACCAGAACCC ACATCCCCCC GTAGCCCTGG GGTTCGCTCG
    CCTGTCCAAT GTGTCTCCCC AGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGA
    CCCAAAGAGC CCCATCTGCA CAGCTACAAG GAAGCCTTTG AGGAGATGGA GGGAACCTCT
    CCGACCAGCC CACCACCCAG TGGGGTGCGT TCCCCTCCAG GTCTGGCCAA GACACCCTTG
    TCTGCTCTGG GCCTGAAGCC CCACAACCCA GCCGACATCC TGCTGCATCC CACTGGTGTC
    CCCAGAAGAC TGATCCAGCC AGAGGAGGAT GAGAGGAAGG TGCTGACAGA GCTTTCTGAA
    GAGCCCCGGA GCTACGTGGA GTCGGTGGTA CGAACGGCGG TGGCTGGGCC CCGGACTCAG
    GAACCTGAGC CCAAGAGCTT TAGCGCCCCA GGTGCGCAGG CGTATGGCCA TGAGACACCC
    CTGAGGATTG GGACCCTGGG AGGCTCCTTT GTCTCCCCTA GCCCCCTCTC CACCAGCAGC
    CCCATCCTCA GTGCTGACAG CACTTCAGTG GGGAGTTTCC CATCCGGGGA GAGCAGCGAC
    CAGGGCGCGC GAACACCTAC CCAGCCTCTG CTGGATTCTG GCTTCCGCTC CGGCAGCCTG
    GGCCAGCCAA GTCCTTTGGC TCAGAGAAAC TACCAGAGCT CTTCTCCTCT CCCAACTGCA
    GGCAGCAGCT ACAGCAGCCC CGACTACTCA CTGCAGCAGT TCAGCTCCCC AGAAGGCCAG
    GCCCGGTCTC AGTTCAGTGT GGCCGGTGTC CACACAGTGC CTGGGAGCCC CCAGGCACGG
    CACAGGACAG TGGGCACCAA TACACCCCCG AGCCCTGGCT TTGGCCGTCG GGCTGTGAAC
    CCCAGCCTGG CTGCCCCCAG CAGCCCCAGT TTGAGCCACC GCCAGGTGAT GGGTCCACTG
    GGAACCGGTT TCCATGGTAA CACGGGCTCC AGCCCCCAGA GCAGTGCAGC AACCACTCCG
    GGAAGCCCCA GTCTGGGTCG CCACCCTGGG GCCCACCAGG TTTCCAACCT CCATGGCAAT
    GTGGTCACCG CTCCTGGGAG CCCCAGCCTG GGCCGGCACC CCGGGGCCCA CCAAGGCACC
    CTGGCCTCCA ATCTCCATAG CAATGCAGTA GCCAGCCCCG GAAGCCCCAG CCTGGGCCGT
    CACTTGGGAG GGTCTGGATC TGTGGTTCCC GGCAGCCCCA GCTTGGACCG GCACGTGCCC
    TATGGTGGCT ACTCCACCCC TGAGGACCGG AGACCCACGC TGTCCCGGCA GAGCAGCGCC
    TCAGGCTACC AGGCTCCTTC CACGCCCTCC TTCCCTGTGT CCCCCGCCTA CTACCCTGGC
    CTGAGCAGCC CCGCCACCTC CCCGTCGCCT GATTCGGCAG CTTTCCGACA AGGGAGCCCC
    ACCCCGGCCT TGCCGGAGAA GCGGAGGATG TCCATGGGCG ACCGAGCGGG CAGCCTCCCC
    AACTACGCCA CCGTCAACGG GAAGGTGTCC TCCTCGCCGG TCGCCAGTGG CATGTCCAGT
    CCCAGCGGGG GCAGCACCGT CTCCTTCTCC CACACTCTGC CCGACTTCTC CAAGTACTCC
    ATGCCAGACA ACAGTCCTGA GACGCGGGCT AAAGTGAAGT TTGTCCAAGA CACTTCCAAG
    TATTGGTACA AGCCTGAGAT CTCCAGAGAA CAGGCCATCG CCCTCCTTAA GGACCAGGAG
    CCAGGGGCTT TCATCATCCG TGACAGCCAC TCCTTCCGAG GAGCCTATGG GCTGGCCATG
    AAGGTGTCTT CGCCCCCTCC GACCATCATG CAGCAGAATA AAAAAGGGGA CATGACCCAC
    GAGCTCGTGA GGCATTTCCT GATAGAGACC GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC
    CCCAATGAGC CAAACTTCGG ATCTCTGTCT GCCCTGGTCT ACCAGCACTC CATCATCCCG
    CTGGCCCTGC CCTGCAAGCT GGTCATTCCA AACCGAGACC CCACAGATGA ATCGAAAGAT
    AGCTCGGGCC CTGCCAACTC AACCAGTGAC CTGCTGAAGC AAGGGGCAGC CTGCAACGTG
    CTCTTCGTCA ACTCTGTGGA CATGGAGTCA CTCACTGGGC CACAGGCCAT CTCCAAAGCC
    ATTTCTGAGA CCCTGGCTGC TGACCCCACA CCAGCTGCCA CCATCGTTCA CTTCAAAGTT
    TCTGCCCAGG GAATTACACT GACTGACAAC CAGAGAAAGC TTTTCTTCAG GCGGCACTAC
    CCCCTCAACA CTGTGACCTT CTGTGACCTG GATCCACAGG AGAGGAAGTG GACAAAGACG
    GAGGGAGGTG CCCCTGCTAA GCTCTTCGGT TTTGTGGCCC GGAAACAGGG CAGCGCCACG
    GACAACGCCT GCCACCTCTT TGCTGAGCTT GACCCCAACC AGCCGGCCTC CGCCATCGTC
    AGCTTTGTCT CCAAGGTCAT GCTGAGTGCC GGCCAGAAGA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854888.1
    CDS229..5613
    Translation

    Target ORF information:

    RefSeq Version XM_024999120.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999120.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAATCCTG CTGGTCGTCA GGACTTGGGG AGTTCCACCA GAGTTACACC AAGTGTCCAG 
    CCTCATCCCC AGCCCATCAG AAACATGAGT ACGAGCCGGA CCACGGAGGA CAGCTGTGAG
    CTGGACCTGG TGTATGTCAC CGAGAGGATC ATCGCCGTCT CCTTCCCGAG CACAGCCAAT
    GAGGAGAACT TCCGGAGCAA CCTCCGAGAG GTGGCCCAGA TGCTCAAGTC CAAACATGGA
    GGCAACTACC TGCTTTTCAA CCTCTCTGAG CGGAGACCTG ACATCACGAA GCTCCACGCC
    AAGGTCCTGG AATTTGGCTG GCCCGACCTC CACACCCCGG CCCTGGAGAA GATCTGCAGC
    GTCTGCAAGG CCATGGACAC CTGGCTCAAT GCAGACCCCC ACAACGTGGT CGTGCTACAC
    AACAAGGGAA ACCGAGGCAG GATCGGCGTT GTGATCGCGG CTTACATGCA CTACAGCAAC
    ATTTCTGCCA GTGCCGACCA GGCCCTGGAC CGGTTTGCGA TGAAACGGTT CTACGAGGAT
    AAGATTGTAC CCATTGGCCA ACCATCCCAG AGGAGGTACG TGCATTATTT CAGCGGCCTG
    CTCTCTGGCT CCATCAAAAT GAACAACAAG CCCTTGTTTC TGCACCACGT GATCATGCAC
    GGCATCCCCA ACTTCGAGTC TAAAGGAGGG TGTCGACCAT TTCTCCGGAT CTACCAGGCC
    ATGCAACCTG TCTACACATC TGGCATCTAC AACGTCCAAG GAGACAGCCA GACCAGCATC
    TGCATCACCA TTGAGCCTGG GCTGCTCTTG AAGGGAGACA TCTTGCTGAA GTGCTACCAC
    AAGAAGTTCC GGAGCCCAGC CCGAGATGTC ATCTTCAGGG TGCAGTTCCA CACCTGTGCC
    ATCCATGACC TGGGAGTTGT CTTTGGGAAG GAGGACCTTG ACGATGCCTT CAAGGACGAT
    CGATTTCCAG AATACGGCAA AGTTGAATTT GTATTTTCTT ATGGACCAGA GAAAATTCAA
    GGCATGGAAC ACCTGGAGAA TGGCCCGAGC GTGTCCGTGG ACTATAACAC CTCTGACCCC
    CTCATCCGCT GGGATTCCTA TGACAACTTC AATGGGCATC GAGATGATGG CATGGAGGAG
    GTTGTGGGCC ATACCCAGGG ACCCCTGGAC GGGAGCCTGT ATGCCAAGGT GAAGAAGAAG
    GACTCCTTGC ACGGCAGCAC TGGGGCTGTC AACGCCACGC GGCCCGTGCT GTCAGCCACC
    CCCAATCACG TGGAGCACAC CCTCTCCGTG AGCAGTGACT CGGGCAACTC CACAGCCTCC
    ACCAAGACAG ACAAGACCGA TGAGCCTGCC CCCGGCCCTG CCAGTGCCCC TGCTGCCCTG
    AGTCCCGAGG AGAAGCGTGA GCTGGACCGC CTGCTCAGTG GCTTTGGCTT GGAGCGGGAG
    AAGCAAGGCG CCATGTACCA CCCTCAGCAT CTCCGGTCCC GCCCAGTGGG AGGCCCTGCT
    GCCCCTTCCT CTGGCCGCCA CATCGTCCCG GCCCAGGTTC ACGTCAACGG TGGGGCCTTA
    GCGTCCGAGC GGGAGACAGA CATCCTGGAT GATGAGCTGC CCAACCAGGA TGGGCATAGC
    GTGGGCAGCA TGGGCACCCT GTCCTCCCTG GATGGAGTCA CCAACACAAG TGAGGGTGGC
    TACCCGGAGG CCCTCTCCCC GCTGACCAAC GGTCTGGACA AGCCCTACCC CATGGAACCT
    ATGGTCAACG GTGGGGGCTA CCCCTATGAG TCTGCCAGCC GGGCAGTCTC TGCCCAGGCT
    GGCCACACAG CCCCCATGAG ACCCTCCTAC TCCACACAGG AGGGTTTAGC TGGCTACCAG
    CGGGAGGGAC CCCACCCAGC CTGGCCACAG TCAGCGACCA CCTCCCACTA TGGCCATGAC
    CCCAACGGTA TGTTTCGCTC TCAATCCTTT CCTGAAACGG AGCCCCAGCT GCCCCCAGCT
    CCAGCCCGTG GTGGGAGCAG CCGGGAGGCT GTGCAGAGGG GTCTGAATTC CTGGCAGCAG
    CAGCAGCAAC AGCAGCAGCA GCAGCAGCAG CCTCGCCCAC CTCCTCGCCA ACAGGAAAGA
    GTCCACTTGG AGAGCCTTGG GCTCAGCAGG CCCAGCCCCC AGCCACTAGC AGAGCCCCCC
    ATGTCTGGCC TCCCTGAGTT CCCTCGCGCA GCCTCCCAGC AGGAGATCGA GCAGTCCATC
    GAAGCCCTCA ATATGCTGAT GCTCGATTTG GAACCAGCCA CGGCGGGCGC CCCCCTACAC
    AAGTCCCAGA GCGTGCCTGG GGCCTGGCCA GGGGCTTCTC CGCTCTCTTC CCAGCCCTTC
    TCTGGCTCCT CTTGCCAGTC CCACCCACTG ACCCAGTCCA GATCTGGCTA CATCCCCAGT
    GGGCATTCCT TGGGAACCCC TGAGCCAGCC CCCCGGGCCC CCCTGGAGTC TGTTCCCACT
    GGCAGGCCTT ACTCACCTTA TGATTATCAG CCATGTCCAA CTGGGCCCAA CCAGAGTTAC
    CATCCAAAGA GCCCGGCCAC CTCCTCCTCG TCTTCCTTCC TTCCAACCAC CCAGAGCTCT
    GTGGGGCCTC AGCAGCCCCC AGCCTCTCTC CCTGGCCTCA CCACTCAGCC TCAGCTTCCA
    CCAAAGGAGG TGACTTCAGA CCCATCCCGA ACTCCAGAGG AGGAACCATT GAACTTGGAA
    GGGCTGGTGG CCCACCGGGT GGCAGCGTAC AATGCCAGGC TGCAGGGCAC TGGGCGGAGA
    GTCAGCTCCT GGCACCCTCC TCTCCACCGG CTCCGATCTT CCTCTCTCTC TGGGGTGCAG
    GCTCGGGAGA AGCAGCCTGC AGAGCCCCCA GCACCTCTCC GGAAGCGGGC TGCCAGTGAT
    GGGCAGTATG AGAACCAGTC ACCAGAACCC ACATCCCCCC GTAGCCCTGG GGTTCGCTCG
    CCTGTCCAAT GTGTCTCCCC AGAGCTGGCT CTTACCATCG CTCTCAATCC TGGAGGGCGA
    CCCAAAGAGC CCCATCTGCA CAGCTACAAG GAAGCCTTTG AGGAGATGGA GGGAACCTCT
    CCGACCAGCC CACCACCCAG TGGGGTGCGT TCCCCTCCAG GTCTGGCCAA GACACCCTTG
    TCTGCTCTGG GCCTGAAGCC CCACAACCCA GCCGACATCC TGCTGCATCC CACTGGTGTC
    CCCAGAAGAC TGATCCAGCC AGAGGAGGAT GAGAGGAAGG TGCTGACAGA GCTTTCTGAA
    GAGCCCCGGA GCTACGTGGA GTCGGTGGTA CGAACGGCGG TGGCTGGGCC CCGGACTCAG
    GAACCTGAGC CCAAGAGCTT TAGCGCCCCA GGTGCGCAGG CGTATGGCCA TGAGACACCC
    CTGAGGATTG GGACCCTGGG AGGCTCCTTT GTCTCCCCTA GCCCCCTCTC CACCAGCAGC
    CCCATCCTCA GTGCTGACAG CACTTCAGTG GGGAGTTTCC CATCCGGGGA GAGCAGCGAC
    CAGGGCGCGC GAACACCTAC CCAGCCTCTG CTGGATTCTG GCTTCCGCTC CGGCAGCCTG
    GGCCAGCCAA GTCCTTTGGC TCAGAGAAAC TACCAGAGCT CTTCTCCTCT CCCAACTGCA
    GGCAGCAGCT ACAGCAGCCC CGACTACTCA CTGCAGCAGT TCAGCTCCCC AGAAGGCCAG
    GCCCGGTCTC AGTTCAGTGT GGCCGGTGTC CACACAGTGC CTGGGAGCCC CCAGGCACGG
    CACAGGACAG TGGGCACCAA TACACCCCCG AGCCCTGGCT TTGGCCGTCG GGCTGTGAAC
    CCCAGCCTGG CTGCCCCCAG CAGCCCCAGT TTGAGCCACC GCCAGGTGAT GGGTCCACTG
    GGAACCGGTT TCCATGGTAA CACGGGCTCC AGCCCCCAGA GCAGTGCAGC AACCACTCCG
    GGAAGCCCCA GTCTGGGTCG CCACCCTGGG GCCCACCAGG TTTCCAACCT CCATGGCAAT
    GTGGTCACCG CTCCTGGGAG CCCCAGCCTG GGCCGGCACC CCGGGGCCCA CCAAGGCACC
    CTGGCCTCCA ATCTCCATAG CAATGCAGTA GCCAGCCCCG GAAGCCCCAG CCTGGGCCGT
    CACTTGGGAG GGTCTGGATC TGTGGTTCCC GGCAGCCCCA GCTTGGACCG GCACGTGCCC
    TATGGTGGCT ACTCCACCCC TGAGGACCGG AGACCCACGC TGTCCCGGCA GAGCAGCGCC
    TCAGGCTACC AGGCTCCTTC CACGCCCTCC TTCCCTGTGT CCCCCGCCTA CTACCCTGGC
    CTGAGCAGCC CCGCCACCTC CCCGTCGCCT GATTCGGCAG CTTTCCGACA AGGGAGCCCC
    ACCCCGGCCT TGCCGGAGAA GCGGAGGATG TCCATGGGCG ACCGAGCGGG CAGCCTCCCC
    AACTACGCCA CCGTCAACGG GAAGGTGTCC TCCTCGCCGG TCGCCAGTGG CATGTCCAGT
    CCCAGCGGGG GCAGCACCGT CTCCTTCTCC CACACTCTGC CCGACTTCTC CAAGTACTCC
    ATGCCAGACA ACAGTCCTGA GACGCGGGCT AAAGTGAAGT TTGTCCAAGA CACTTCCAAG
    TATTGGTACA AGCCTGAGAT CTCCAGAGAA CAGGCCATCG CCCTCCTTAA GGACCAGGAG
    CCAGGGGCTT TCATCATCCG TGACAGCCAC TCCTTCCGAG GAGCCTATGG GCTGGCCATG
    AAGGTGTCTT CGCCCCCTCC GACCATCATG CAGCAGAATA AAAAAGGGGA CATGACCCAC
    GAGCTCGTGA GGCATTTCCT GATAGAGACC GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC
    CCCAATGAGC CAAACTTCGG ATCTCTGTCT GCCCTGGTCT ACCAGCACTC CATCATCCCG
    CTGGCCCTGC CCTGCAAGCT GGTCATTCCA AACCGAGACC CCACAGATGA ATCGAAAGAT
    AGCTCGGGCC CTGCCAACTC AACCAGTGAC CTGCTGAAGC AAGGGGCAGC CTGCAACGTG
    CTCTTCGTCA ACTCTGTGGA CATGGAGTCA CTCACTGGGC CACAGGCCAT CTCCAAAGCC
    ATTTCTGAGA CCCTGGCTGC TGACCCCACA CCAGCTGCCA CCATCGTTCA CTTCAAAGTT
    TCTGCCCAGG GAATTACACT GACTGACAAC CAGAGAAAGC TTTTCTTCAG GCGGCACTAC
    CCCCTCAACA CTGTGACCTT CTGTGACCTG GATCCACAGG AGAGGAAGTG GACAAAGACG
    GAGGGAGGTG CCCCTGCTAA GCTCTTCGGT TTTGTGGCCC GGAAACAGGG CAGCGCCACG
    GACAACGCCT GCCACCTCTT TGCTGAGCTT GACCCCAACC AGCCGGCCTC CGCCATCGTC
    AGCTTTGTCT CCAAGGTCAT GCTGAGTGCC GGCCAGAAGA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227177
    Clone ID Related Accession (Same CDS sequence) XM_024999124.1
    Accession Version XM_024999124.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5379bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCCTCTCA AATTAGTGGA AAAGAGTTCC ACCAGAGTTA CACCAAGTGT CCAGCCTCAT 
    CCCCAGCCCA TCAGAAACAT GAGTACGAGC CGGACCACGG AGGACAGCTG TGAGCTGGAC
    CTGGTGTATG TCACCGAGAG GATCATCGCC GTCTCCTTCC CGAGCACAGC CAATGAGGAG
    AACTTCCGGA GCAACCTCCG AGAGGTGGCC CAGATGCTCA AGTCCAAACA TGGAGGCAAC
    TACCTGCTTT TCAACCTCTC TGAGCGGAGA CCTGACATCA CGAAGCTCCA CGCCAAGGTC
    CTGGAATTTG GCTGGCCCGA CCTCCACACC CCGGCCCTGG AGAAGATCTG CAGCGTCTGC
    AAGGCCATGG ACACCTGGCT CAATGCAGAC CCCCACAACG TGGTCGTGCT ACACAACAAG
    GGAAACCGAG GCAGGATCGG CGTTGTGATC GCGGCTTACA TGCACTACAG CAACATTTCT
    GCCAGTGCCG ACCAGGCCCT GGACCGGTTT GCGATGAAAC GGTTCTACGA GGATAAGATT
    GTACCCATTG GCCAACCATC CCAGAGGAGG TACGTGCATT ATTTCAGCGG CCTGCTCTCT
    GGCTCCATCA AAATGAACAA CAAGCCCTTG TTTCTGCACC ACGTGATCAT GCACGGCATC
    CCCAACTTCG AGTCTAAAGG AGGGTGTCGA CCATTTCTCC GGATCTACCA GGCCATGCAA
    CCTGTCTACA CATCTGGCAT CTACAACGTC CAAGGAGACA GCCAGACCAG CATCTGCATC
    ACCATTGAGC CTGGGCTGCT CTTGAAGGGA GACATCTTGC TGAAGTGCTA CCACAAGAAG
    TTCCGGAGCC CAGCCCGAGA TGTCATCTTC AGGGTGCAGT TCCACACCTG TGCCATCCAT
    GACCTGGGAG TTGTCTTTGG GAAGGAGGAC CTTGACGATG CCTTCAAGGA CGATCGATTT
    CCAGAATACG GCAAAGTTGA ATTTGTATTT TCTTATGGAC CAGAGAAAAT TCAAGGCATG
    GAACACCTGG AGAATGGCCC GAGCGTGTCC GTGGACTATA ACACCTCTGA CCCCCTCATC
    CGCTGGGATT CCTATGACAA CTTCAATGGG CATCGAGATG ATGGCATGGA GGAGGTTGTG
    GGCCATACCC AGGGACCCCT GGACGGGAGC CTGTATGCCA AGGTGAAGAA GAAGGACTCC
    TTGCACGGCA GCACTGGGGC TGTCAACGCC ACGCGGCCCG TGCTGTCAGC CACCCCCAAT
    CACGTGGAGC ACACCCTCTC CGTGAGCAGT GACTCGGGCA ACTCCACAGC CTCCACCAAG
    ACAGACAAGA CCGATGAGCC TGCCCCCGGC CCTGCCAGTG CCCCTGCTGC CCTGAGTCCC
    GAGGAGAAGC GTGAGCTGGA CCGCCTGCTC AGTGGCTTTG GCTTGGAGCG GGAGAAGCAA
    GGCGCCATGT ACCACCCTCA GCATCTCCGG TCCCGCCCAG TGGGAGGCCC TGCTGCCCCT
    TCCTCTGGCC GCCACATCGT CCCGGCCCAG GTTCACGTCA ACGGTGGGGC CTTAGCGTCC
    GAGCGGGAGA CAGACATCCT GGATGATGAG CTGCCCAACC AGGATGGGCA TAGCGTGGGC
    AGCATGGGCA CCCTGTCCTC CCTGGATGGA GTCACCAACA CAAGTGAGGG TGGCTACCCG
    GAGGCCCTCT CCCCGCTGAC CAACGGTCTG GACAAGCCCT ACCCCATGGA ACCTATGGTC
    AACGGTGGGG GCTACCCCTA TGAGTCTGCC AGCCGGGCAG TCTCTGCCCA GGCTGGCCAC
    ACAGCCCCCA TGAGACCCTC CTACTCCACA CAGGAGGGTT TAGCTGGCTA CCAGCGGGAG
    GGACCCCACC CAGCCTGGCC ACAGTCAGCG ACCACCTCCC ACTATGGCCA TGACCCCAAC
    GGTATGTTTC GCTCTCAATC CTTTCCTGAA ACGGAGCCCC AGCTGCCCCC AGCTCCAGCC
    CGTGGTGGGA GCAGCCGGGA GGCTGTGCAG AGGGGTCTGA ATTCCTGGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAGCA GCAGCCTCGC CCACCTCCTC GCCAACAGGA AAGAGTCCAC
    TTGGAGAGCC TTGGGCTCAG CAGGCCCAGC CCCCAGCCAC TAGCAGAGCC CCCCATGTCT
    GGCCTCCCTG AGTTCCCTCG CGCAGCCTCC CAGCAGGAGA TCGAGCAGTC CATCGAAGCC
    CTCAATATGC TGATGCTCGA TTTGGAACCA GCCACGGCGG GCGCCCCCCT ACACAAGTCC
    CAGAGCGTGC CTGGGGCCTG GCCAGGGGCT TCTCCGCTCT CTTCCCAGCC CTTCTCTGGC
    TCCTCTTGCC AGTCCCACCC ACTGACCCAG TCCAGATCTG GCTACATCCC CAGTGGGCAT
    TCCTTGGGAA CCCCTGAGCC AGCCCCCCGG GCCCCCCTGG AGTCTGTTCC CACTGGCAGG
    CCTTACTCAC CTTATGATTA TCAGCCATGT CCAACTGGGC CCAACCAGAG TTACCATCCA
    AAGAGCCCGG CCACCTCCTC CTCGTCTTCC TTCCTTCCAA CCACCCAGAG CTCTGTGGGG
    CCTCAGCAGC CCCCAGCCTC TCTCCCTGGC CTCACCACTC AGCCTCAGCT TCCACCAAAG
    GAGGTGACTT CAGACCCATC CCGAACTCCA GAGGAGGAAC CATTGAACTT GGAAGGGCTG
    GTGGCCCACC GGGTGGCAGC GTACAATGCC AGGCTGCAGG GCACTGGGCG GAGAGTCAGC
    TCCTGGCACC CTCCTCTCCA CCGGCTCCGA TCTTCCTCTC TCTCTGGGGT GCAGGCTCGG
    GAGAAGCAGC CTGCAGAGCC CCCAGCACCT CTCCGGAAGC GGGCTGCCAG TGATGGGCAG
    TATGAGAACC AGTCACCAGA ACCCACATCC CCCCGTAGCC CTGGGGTTCG CTCGCCTGTC
    CAATGTGTCT CCCCAGAGCT GGCTCTTACC ATCGCTCTCA ATCCTGGAGG GCGACCCAAA
    GAGCCCCATC TGCACAGCTA CAAGGAAGCC TTTGAGGAGA TGGAGGGAAC CTCTCCGACC
    AGCCCACCAC CCAGTGGGGT GCGTTCCCCT CCAGGTCTGG CCAAGACACC CTTGTCTGCT
    CTGGGCCTGA AGCCCCACAA CCCAGCCGAC ATCCTGCTGC ATCCCACTGG TGTCCCCAGA
    AGACTGATCC AGCCAGAGGA GGATGAGAGG AAGGTGCTGA CAGAGCTTTC TGAAGAGCCC
    CGGAGCTACG TGGAGTCGGT GGTACGAACG GCGGTGGCTG GGCCCCGGAC TCAGGAACCT
    GAGCCCAAGA GCTTTAGCGC CCCAGGTGCG CAGGCGTATG GCCATGAGAC ACCCCTGAGG
    ATTGGGACCC TGGGAGGCTC CTTTGTCTCC CCTAGCCCCC TCTCCACCAG CAGCCCCATC
    CTCAGTGCTG ACAGCACTTC AGTGGGGAGT TTCCCATCCG GGGAGAGCAG CGACCAGGGC
    GCGCGAACAC CTACCCAGCC TCTGCTGGAT TCTGGCTTCC GCTCCGGCAG CCTGGGCCAG
    CCAAGTCCTT TGGCTCAGAG AAACTACCAG AGCTCTTCTC CTCTCCCAAC TGCAGGCAGC
    AGCTACAGCA GCCCCGACTA CTCACTGCAG CAGTTCAGCT CCCCAGAAGG CCAGGCCCGG
    TCTCAGTTCA GTGTGGCCGG TGTCCACACA GTGCCTGGGA GCCCCCAGGC ACGGCACAGG
    ACAGTGGGCA CCAATACACC CCCGAGCCCT GGCTTTGGCC GTCGGGCTGT GAACCCCAGC
    CTGGCTGCCC CCAGCAGCCC CAGTTTGAGC CACCGCCAGG TGATGGGTCC ACTGGGAACC
    GGTTTCCATG GTAACACGGG CTCCAGCCCC CAGAGCAGTG CAGCAACCAC TCCGGGAAGC
    CCCAGTCTGG GTCGCCACCC TGGGGCCCAC CAGGTTTCCA ACCTCCATGG CAATGTGGTC
    ACCGCTCCTG GGAGCCCCAG CCTGGGCCGG CACCCCGGGG CCCACCAAGG CACCCTGGCC
    TCCAATCTCC ATAGCAATGC AGTAGCCAGC CCCGGAAGCC CCAGCCTGGG CCGTCACTTG
    GGAGGGTCTG GATCTGTGGT TCCCGGCAGC CCCAGCTTGG ACCGGCACGT GCCCTATGGT
    GGCTACTCCA CCCCTGAGGA CCGGAGACCC ACGCTGTCCC GGCAGAGCAG CGCCTCAGGC
    TACCAGGCTC CTTCCACGCC CTCCTTCCCT GTGTCCCCCG CCTACTACCC TGGCCTGAGC
    AGCCCCGCCA CCTCCCCGTC GCCTGATTCG GCAGCTTTCC GACAAGGGAG CCCCACCCCG
    GCCTTGCCGG AGAAGCGGAG GATGTCCATG GGCGACCGAG CGGGCAGCCT CCCCAACTAC
    GCCACCGTCA ACGGGAAGGT GTCCTCCTCG CCGGTCGCCA GTGGCATGTC CAGTCCCAGC
    GGGGGCAGCA CCGTCTCCTT CTCCCACACT CTGCCCGACT TCTCCAAGTA CTCCATGCCA
    GACAACAGTC CTGAGACGCG GGCTAAAGTG AAGTTTGTCC AAGACACTTC CAAGTATTGG
    TACAAGCCTG AGATCTCCAG AGAACAGGCC ATCGCCCTCC TTAAGGACCA GGAGCCAGGG
    GCTTTCATCA TCCGTGACAG CCACTCCTTC CGAGGAGCCT ATGGGCTGGC CATGAAGGTG
    TCTTCGCCCC CTCCGACCAT CATGCAGCAG AATAAAAAAG GGGACATGAC CCACGAGCTC
    GTGAGGCATT TCCTGATAGA GACCGGCCCC AGAGGAGTCA AGCTCAAGGG CTGCCCCAAT
    GAGCCAAACT TCGGATCTCT GTCTGCCCTG GTCTACCAGC ACTCCATCAT CCCGCTGGCC
    CTGCCCTGCA AGCTGGTCAT TCCAAACCGA GACCCCACAG ATGAATCGAA AGATAGCTCG
    GGCCCTGCCA ACTCAACCAG TGACCTGCTG AAGCAAGGGG CAGCCTGCAA CGTGCTCTTC
    GTCAACTCTG TGGACATGGA GTCACTCACT GGGCCACAGG CCATCTCCAA AGCCATTTCT
    GAGACCCTGG CTGCTGACCC CACACCAGCT GCCACCATCG TTCACTTCAA AGTTTCTGCC
    CAGGGAATTA CACTGACTGA CAACCAGAGA AAGCTTTTCT TCAGGCGGCA CTACCCCCTC
    AACACTGTGA CCTTCTGTGA CCTGGATCCA CAGGAGAGGA AGTGGACAAA GACGGAGGGA
    GGTGCCCCTG CTAAGCTCTT CGGTTTTGTG GCCCGGAAAC AGGGCAGCGC CACGGACAAC
    GCCTGCCACC TCTTTGCTGA GCTTGACCCC AACCAGCCGG CCTCCGCCAT CGTCAGCTTT
    GTCTCCAAGG TCATGCTGAG TGCCGGCCAG AAGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854892.1
    CDS121..5499
    Translation

    Target ORF information:

    RefSeq Version XM_024999124.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999124.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCCTCTCA AATTAGTGGA AAAGAGTTCC ACCAGAGTTA CACCAAGTGT CCAGCCTCAT 
    CCCCAGCCCA TCAGAAACAT GAGTACGAGC CGGACCACGG AGGACAGCTG TGAGCTGGAC
    CTGGTGTATG TCACCGAGAG GATCATCGCC GTCTCCTTCC CGAGCACAGC CAATGAGGAG
    AACTTCCGGA GCAACCTCCG AGAGGTGGCC CAGATGCTCA AGTCCAAACA TGGAGGCAAC
    TACCTGCTTT TCAACCTCTC TGAGCGGAGA CCTGACATCA CGAAGCTCCA CGCCAAGGTC
    CTGGAATTTG GCTGGCCCGA CCTCCACACC CCGGCCCTGG AGAAGATCTG CAGCGTCTGC
    AAGGCCATGG ACACCTGGCT CAATGCAGAC CCCCACAACG TGGTCGTGCT ACACAACAAG
    GGAAACCGAG GCAGGATCGG CGTTGTGATC GCGGCTTACA TGCACTACAG CAACATTTCT
    GCCAGTGCCG ACCAGGCCCT GGACCGGTTT GCGATGAAAC GGTTCTACGA GGATAAGATT
    GTACCCATTG GCCAACCATC CCAGAGGAGG TACGTGCATT ATTTCAGCGG CCTGCTCTCT
    GGCTCCATCA AAATGAACAA CAAGCCCTTG TTTCTGCACC ACGTGATCAT GCACGGCATC
    CCCAACTTCG AGTCTAAAGG AGGGTGTCGA CCATTTCTCC GGATCTACCA GGCCATGCAA
    CCTGTCTACA CATCTGGCAT CTACAACGTC CAAGGAGACA GCCAGACCAG CATCTGCATC
    ACCATTGAGC CTGGGCTGCT CTTGAAGGGA GACATCTTGC TGAAGTGCTA CCACAAGAAG
    TTCCGGAGCC CAGCCCGAGA TGTCATCTTC AGGGTGCAGT TCCACACCTG TGCCATCCAT
    GACCTGGGAG TTGTCTTTGG GAAGGAGGAC CTTGACGATG CCTTCAAGGA CGATCGATTT
    CCAGAATACG GCAAAGTTGA ATTTGTATTT TCTTATGGAC CAGAGAAAAT TCAAGGCATG
    GAACACCTGG AGAATGGCCC GAGCGTGTCC GTGGACTATA ACACCTCTGA CCCCCTCATC
    CGCTGGGATT CCTATGACAA CTTCAATGGG CATCGAGATG ATGGCATGGA GGAGGTTGTG
    GGCCATACCC AGGGACCCCT GGACGGGAGC CTGTATGCCA AGGTGAAGAA GAAGGACTCC
    TTGCACGGCA GCACTGGGGC TGTCAACGCC ACGCGGCCCG TGCTGTCAGC CACCCCCAAT
    CACGTGGAGC ACACCCTCTC CGTGAGCAGT GACTCGGGCA ACTCCACAGC CTCCACCAAG
    ACAGACAAGA CCGATGAGCC TGCCCCCGGC CCTGCCAGTG CCCCTGCTGC CCTGAGTCCC
    GAGGAGAAGC GTGAGCTGGA CCGCCTGCTC AGTGGCTTTG GCTTGGAGCG GGAGAAGCAA
    GGCGCCATGT ACCACCCTCA GCATCTCCGG TCCCGCCCAG TGGGAGGCCC TGCTGCCCCT
    TCCTCTGGCC GCCACATCGT CCCGGCCCAG GTTCACGTCA ACGGTGGGGC CTTAGCGTCC
    GAGCGGGAGA CAGACATCCT GGATGATGAG CTGCCCAACC AGGATGGGCA TAGCGTGGGC
    AGCATGGGCA CCCTGTCCTC CCTGGATGGA GTCACCAACA CAAGTGAGGG TGGCTACCCG
    GAGGCCCTCT CCCCGCTGAC CAACGGTCTG GACAAGCCCT ACCCCATGGA ACCTATGGTC
    AACGGTGGGG GCTACCCCTA TGAGTCTGCC AGCCGGGCAG TCTCTGCCCA GGCTGGCCAC
    ACAGCCCCCA TGAGACCCTC CTACTCCACA CAGGAGGGTT TAGCTGGCTA CCAGCGGGAG
    GGACCCCACC CAGCCTGGCC ACAGTCAGCG ACCACCTCCC ACTATGGCCA TGACCCCAAC
    GGTATGTTTC GCTCTCAATC CTTTCCTGAA ACGGAGCCCC AGCTGCCCCC AGCTCCAGCC
    CGTGGTGGGA GCAGCCGGGA GGCTGTGCAG AGGGGTCTGA ATTCCTGGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAGCA GCAGCCTCGC CCACCTCCTC GCCAACAGGA AAGAGTCCAC
    TTGGAGAGCC TTGGGCTCAG CAGGCCCAGC CCCCAGCCAC TAGCAGAGCC CCCCATGTCT
    GGCCTCCCTG AGTTCCCTCG CGCAGCCTCC CAGCAGGAGA TCGAGCAGTC CATCGAAGCC
    CTCAATATGC TGATGCTCGA TTTGGAACCA GCCACGGCGG GCGCCCCCCT ACACAAGTCC
    CAGAGCGTGC CTGGGGCCTG GCCAGGGGCT TCTCCGCTCT CTTCCCAGCC CTTCTCTGGC
    TCCTCTTGCC AGTCCCACCC ACTGACCCAG TCCAGATCTG GCTACATCCC CAGTGGGCAT
    TCCTTGGGAA CCCCTGAGCC AGCCCCCCGG GCCCCCCTGG AGTCTGTTCC CACTGGCAGG
    CCTTACTCAC CTTATGATTA TCAGCCATGT CCAACTGGGC CCAACCAGAG TTACCATCCA
    AAGAGCCCGG CCACCTCCTC CTCGTCTTCC TTCCTTCCAA CCACCCAGAG CTCTGTGGGG
    CCTCAGCAGC CCCCAGCCTC TCTCCCTGGC CTCACCACTC AGCCTCAGCT TCCACCAAAG
    GAGGTGACTT CAGACCCATC CCGAACTCCA GAGGAGGAAC CATTGAACTT GGAAGGGCTG
    GTGGCCCACC GGGTGGCAGC GTACAATGCC AGGCTGCAGG GCACTGGGCG GAGAGTCAGC
    TCCTGGCACC CTCCTCTCCA CCGGCTCCGA TCTTCCTCTC TCTCTGGGGT GCAGGCTCGG
    GAGAAGCAGC CTGCAGAGCC CCCAGCACCT CTCCGGAAGC GGGCTGCCAG TGATGGGCAG
    TATGAGAACC AGTCACCAGA ACCCACATCC CCCCGTAGCC CTGGGGTTCG CTCGCCTGTC
    CAATGTGTCT CCCCAGAGCT GGCTCTTACC ATCGCTCTCA ATCCTGGAGG GCGACCCAAA
    GAGCCCCATC TGCACAGCTA CAAGGAAGCC TTTGAGGAGA TGGAGGGAAC CTCTCCGACC
    AGCCCACCAC CCAGTGGGGT GCGTTCCCCT CCAGGTCTGG CCAAGACACC CTTGTCTGCT
    CTGGGCCTGA AGCCCCACAA CCCAGCCGAC ATCCTGCTGC ATCCCACTGG TGTCCCCAGA
    AGACTGATCC AGCCAGAGGA GGATGAGAGG AAGGTGCTGA CAGAGCTTTC TGAAGAGCCC
    CGGAGCTACG TGGAGTCGGT GGTACGAACG GCGGTGGCTG GGCCCCGGAC TCAGGAACCT
    GAGCCCAAGA GCTTTAGCGC CCCAGGTGCG CAGGCGTATG GCCATGAGAC ACCCCTGAGG
    ATTGGGACCC TGGGAGGCTC CTTTGTCTCC CCTAGCCCCC TCTCCACCAG CAGCCCCATC
    CTCAGTGCTG ACAGCACTTC AGTGGGGAGT TTCCCATCCG GGGAGAGCAG CGACCAGGGC
    GCGCGAACAC CTACCCAGCC TCTGCTGGAT TCTGGCTTCC GCTCCGGCAG CCTGGGCCAG
    CCAAGTCCTT TGGCTCAGAG AAACTACCAG AGCTCTTCTC CTCTCCCAAC TGCAGGCAGC
    AGCTACAGCA GCCCCGACTA CTCACTGCAG CAGTTCAGCT CCCCAGAAGG CCAGGCCCGG
    TCTCAGTTCA GTGTGGCCGG TGTCCACACA GTGCCTGGGA GCCCCCAGGC ACGGCACAGG
    ACAGTGGGCA CCAATACACC CCCGAGCCCT GGCTTTGGCC GTCGGGCTGT GAACCCCAGC
    CTGGCTGCCC CCAGCAGCCC CAGTTTGAGC CACCGCCAGG TGATGGGTCC ACTGGGAACC
    GGTTTCCATG GTAACACGGG CTCCAGCCCC CAGAGCAGTG CAGCAACCAC TCCGGGAAGC
    CCCAGTCTGG GTCGCCACCC TGGGGCCCAC CAGGTTTCCA ACCTCCATGG CAATGTGGTC
    ACCGCTCCTG GGAGCCCCAG CCTGGGCCGG CACCCCGGGG CCCACCAAGG CACCCTGGCC
    TCCAATCTCC ATAGCAATGC AGTAGCCAGC CCCGGAAGCC CCAGCCTGGG CCGTCACTTG
    GGAGGGTCTG GATCTGTGGT TCCCGGCAGC CCCAGCTTGG ACCGGCACGT GCCCTATGGT
    GGCTACTCCA CCCCTGAGGA CCGGAGACCC ACGCTGTCCC GGCAGAGCAG CGCCTCAGGC
    TACCAGGCTC CTTCCACGCC CTCCTTCCCT GTGTCCCCCG CCTACTACCC TGGCCTGAGC
    AGCCCCGCCA CCTCCCCGTC GCCTGATTCG GCAGCTTTCC GACAAGGGAG CCCCACCCCG
    GCCTTGCCGG AGAAGCGGAG GATGTCCATG GGCGACCGAG CGGGCAGCCT CCCCAACTAC
    GCCACCGTCA ACGGGAAGGT GTCCTCCTCG CCGGTCGCCA GTGGCATGTC CAGTCCCAGC
    GGGGGCAGCA CCGTCTCCTT CTCCCACACT CTGCCCGACT TCTCCAAGTA CTCCATGCCA
    GACAACAGTC CTGAGACGCG GGCTAAAGTG AAGTTTGTCC AAGACACTTC CAAGTATTGG
    TACAAGCCTG AGATCTCCAG AGAACAGGCC ATCGCCCTCC TTAAGGACCA GGAGCCAGGG
    GCTTTCATCA TCCGTGACAG CCACTCCTTC CGAGGAGCCT ATGGGCTGGC CATGAAGGTG
    TCTTCGCCCC CTCCGACCAT CATGCAGCAG AATAAAAAAG GGGACATGAC CCACGAGCTC
    GTGAGGCATT TCCTGATAGA GACCGGCCCC AGAGGAGTCA AGCTCAAGGG CTGCCCCAAT
    GAGCCAAACT TCGGATCTCT GTCTGCCCTG GTCTACCAGC ACTCCATCAT CCCGCTGGCC
    CTGCCCTGCA AGCTGGTCAT TCCAAACCGA GACCCCACAG ATGAATCGAA AGATAGCTCG
    GGCCCTGCCA ACTCAACCAG TGACCTGCTG AAGCAAGGGG CAGCCTGCAA CGTGCTCTTC
    GTCAACTCTG TGGACATGGA GTCACTCACT GGGCCACAGG CCATCTCCAA AGCCATTTCT
    GAGACCCTGG CTGCTGACCC CACACCAGCT GCCACCATCG TTCACTTCAA AGTTTCTGCC
    CAGGGAATTA CACTGACTGA CAACCAGAGA AAGCTTTTCT TCAGGCGGCA CTACCCCCTC
    AACACTGTGA CCTTCTGTGA CCTGGATCCA CAGGAGAGGA AGTGGACAAA GACGGAGGGA
    GGTGCCCCTG CTAAGCTCTT CGGTTTTGTG GCCCGGAAAC AGGGCAGCGC CACGGACAAC
    GCCTGCCACC TCTTTGCTGA GCTTGACCCC AACCAGCCGG CCTCCGCCAT CGTCAGCTTT
    GTCTCCAAGG TCATGCTGAG TGCCGGCCAG AAGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227178
    Clone ID Related Accession (Same CDS sequence) XM_024999129.1
    Accession Version XM_024999129.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GTGCGTTCCC CTCCAGGTCT GGCCAAGACA CCCTTGTCTG CTCTGGGCCT GAAGCCCCAC
    AACCCAGCCG ACATCCTGCT GCATCCCACT GGTGTCCCCA GAAGACTGAT CCAGCCAGAG
    GAGGATGAGA GGAAGGTGCT GACAGAGCTT TCTGAAGAGC CCCGGAGCTA CGTGGAGTCG
    GTGGTACGAA CGGCGGTGGC TGGGCCCCGG ACTCAGGAAC CTGAGCCCAA GAGCTTTAGC
    GCCCCAGGTG CGCAGGCGTA TGGCCATGAG ACACCCCTGA GGATTGGGAC CCTGGGAGGC
    TCCTTTGTCT CCCCTAGCCC CCTCTCCACC AGCAGCCCCA TCCTCAGTGC TGACAGCACT
    TCAGTGGGGA GTTTCCCATC CGGGGAGAGC AGCGACCAGG GCGCGCGAAC ACCTACCCAG
    CCTCTGCTGG ATTCTGGCTT CCGCTCCGGC AGCCTGGGCC AGCCAAGTCC TTTGGCTCAG
    AGAAACTACC AGAGCTCTTC TCCTCTCCCA ACTGCAGGCA GCAGCTACAG CAGCCCCGAC
    TACTCACTGC AGCAGTTCAG CTCCCCAGAA GGCCAGGCCC GGTCTCAGTT CAGTGTGGCC
    GGTGTCCACA CAGTGCCTGG GAGCCCCCAG GCACGGCACA GGACAGTGGG CACCAATACA
    CCCCCGAGCC CTGGCTTTGG CCGTCGGGCT GTGAACCCCA GCCTGGCTGC CCCCAGCAGC
    CCCAGTTTGA GCCACCGCCA GGTGATGGGT CCACTGGGAA CCGGTTTCCA TGGTAACACG
    GGCTCCAGCC CCCAGAGCAG TGCAGCAACC ACTCCGGGAA GCCCCAGTCT GGGTCGCCAC
    CCTGGGGCCC ACCAGGTTTC CAACCTCCAT GGCAATGTGG TCACCGCTCC TGGGAGCCCC
    AGCCTGGGCC GGCACCCCGG GGCCCACCAA GGCACCCTGG CCTCCAATCT CCATAGCAAT
    GCAGTAGCCA GCCCCGGAAG CCCCAGCCTG GGCCGTCACT TGGGAGGGTC TGGATCTGTG
    GTTCCCGGCA GCCCCAGCTT GGACCGGCAC GTGCCCTATG GTGGCTACTC CACCCCTGAG
    GACCGGAGAC CCACGCTGTC CCGGCAGAGC AGCGCCTCAG GCTACCAGGC TCCTTCCACG
    CCCTCCTTCC CTGTGTCCCC CGCCTACTAC CCTGGCCTGA GCAGCCCCGC CACCTCCCCG
    TCGCCTGATT CGGCAGCTTT CCGACAAGGG AGCCCCACCC CGGCCTTGCC GGAGAAGCGG
    AGGATGTCCA TGGGCGACCG AGCGGGCAGC CTCCCCAACT ACGCCACCGT CAACGGGAAG
    GTGTCCTCCT CGCCGGTCGC CAGTGGCATG TCCAGTCCCA GCGGGGGCAG CACCGTCTCC
    TTCTCCCACA CTCTGCCCGA CTTCTCCAAG TACTCCATGC CAGACAACAG TCCTGAGACG
    CGGGCTAAAG TGAAGTTTGT CCAAGACACT TCCAAGTATT GGTACAAGCC TGAGATCTCC
    AGAGAACAGG CCATCGCCCT CCTTAAGGAC CAGGAGCCAG GGGCTTTCAT CATCCGTGAC
    AGCCACTCCT TCCGAGGAGC CTATGGGCTG GCCATGAAGG TGTCTTCGCC CCCTCCGACC
    ATCATGCAGC AGAATAAAAA AGGGGACATG ACCCACGAGC TCGTGAGGCA TTTCCTGATA
    GAGACCGGCC CCAGAGGAGT CAAGCTCAAG GGCTGCCCCA ATGAGCCAAA CTTCGGATCT
    CTGTCTGCCC TGGTCTACCA GCACTCCATC ATCCCGCTGG CCCTGCCCTG CAAGCTGGTC
    ATTCCAAACC GAGACCCCAC AGATGAATCG AAAGATAGCT CGGGCCCTGC CAACTCAACC
    AGTGACCTGC TGAAGCAAGG GGCAGCCTGC AACGTGCTCT TCGTCAACTC TGTGGACATG
    GAGTCACTCA CTGGGCCACA GGCCATCTCC AAAGCCATTT CTGAGACCCT GGCTGCTGAC
    CCCACACCAG CTGCCACCAT CGTTCACTTC AAAGTTTCTG CCCAGGGAAT TACACTGACT
    GACAACCAGA GAAAGCTTTT CTTCAGGCGG CACTACCCCC TCAACACTGT GACCTTCTGT
    GACCTGGATC CACAGGAGAG GAAGTGGACA AAGACGGAGG GAGGTGCCCC TGCTAAGCTC
    TTCGGTTTTG TGGCCCGGAA ACAGGGCAGC GCCACGGACA ACGCCTGCCA CCTCTTTGCT
    GAGCTTGACC CCAACCAGCC GGCCTCCGCC ATCGTCAGCT TTGTCTCCAA GGTCATGCTG
    AGTGCCGGCC AGAAGAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854897.1
    CDS343..5283
    Translation

    Target ORF information:

    RefSeq Version XM_024999129.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999129.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GTGCGTTCCC CTCCAGGTCT GGCCAAGACA CCCTTGTCTG CTCTGGGCCT GAAGCCCCAC
    AACCCAGCCG ACATCCTGCT GCATCCCACT GGTGTCCCCA GAAGACTGAT CCAGCCAGAG
    GAGGATGAGA GGAAGGTGCT GACAGAGCTT TCTGAAGAGC CCCGGAGCTA CGTGGAGTCG
    GTGGTACGAA CGGCGGTGGC TGGGCCCCGG ACTCAGGAAC CTGAGCCCAA GAGCTTTAGC
    GCCCCAGGTG CGCAGGCGTA TGGCCATGAG ACACCCCTGA GGATTGGGAC CCTGGGAGGC
    TCCTTTGTCT CCCCTAGCCC CCTCTCCACC AGCAGCCCCA TCCTCAGTGC TGACAGCACT
    TCAGTGGGGA GTTTCCCATC CGGGGAGAGC AGCGACCAGG GCGCGCGAAC ACCTACCCAG
    CCTCTGCTGG ATTCTGGCTT CCGCTCCGGC AGCCTGGGCC AGCCAAGTCC TTTGGCTCAG
    AGAAACTACC AGAGCTCTTC TCCTCTCCCA ACTGCAGGCA GCAGCTACAG CAGCCCCGAC
    TACTCACTGC AGCAGTTCAG CTCCCCAGAA GGCCAGGCCC GGTCTCAGTT CAGTGTGGCC
    GGTGTCCACA CAGTGCCTGG GAGCCCCCAG GCACGGCACA GGACAGTGGG CACCAATACA
    CCCCCGAGCC CTGGCTTTGG CCGTCGGGCT GTGAACCCCA GCCTGGCTGC CCCCAGCAGC
    CCCAGTTTGA GCCACCGCCA GGTGATGGGT CCACTGGGAA CCGGTTTCCA TGGTAACACG
    GGCTCCAGCC CCCAGAGCAG TGCAGCAACC ACTCCGGGAA GCCCCAGTCT GGGTCGCCAC
    CCTGGGGCCC ACCAGGTTTC CAACCTCCAT GGCAATGTGG TCACCGCTCC TGGGAGCCCC
    AGCCTGGGCC GGCACCCCGG GGCCCACCAA GGCACCCTGG CCTCCAATCT CCATAGCAAT
    GCAGTAGCCA GCCCCGGAAG CCCCAGCCTG GGCCGTCACT TGGGAGGGTC TGGATCTGTG
    GTTCCCGGCA GCCCCAGCTT GGACCGGCAC GTGCCCTATG GTGGCTACTC CACCCCTGAG
    GACCGGAGAC CCACGCTGTC CCGGCAGAGC AGCGCCTCAG GCTACCAGGC TCCTTCCACG
    CCCTCCTTCC CTGTGTCCCC CGCCTACTAC CCTGGCCTGA GCAGCCCCGC CACCTCCCCG
    TCGCCTGATT CGGCAGCTTT CCGACAAGGG AGCCCCACCC CGGCCTTGCC GGAGAAGCGG
    AGGATGTCCA TGGGCGACCG AGCGGGCAGC CTCCCCAACT ACGCCACCGT CAACGGGAAG
    GTGTCCTCCT CGCCGGTCGC CAGTGGCATG TCCAGTCCCA GCGGGGGCAG CACCGTCTCC
    TTCTCCCACA CTCTGCCCGA CTTCTCCAAG TACTCCATGC CAGACAACAG TCCTGAGACG
    CGGGCTAAAG TGAAGTTTGT CCAAGACACT TCCAAGTATT GGTACAAGCC TGAGATCTCC
    AGAGAACAGG CCATCGCCCT CCTTAAGGAC CAGGAGCCAG GGGCTTTCAT CATCCGTGAC
    AGCCACTCCT TCCGAGGAGC CTATGGGCTG GCCATGAAGG TGTCTTCGCC CCCTCCGACC
    ATCATGCAGC AGAATAAAAA AGGGGACATG ACCCACGAGC TCGTGAGGCA TTTCCTGATA
    GAGACCGGCC CCAGAGGAGT CAAGCTCAAG GGCTGCCCCA ATGAGCCAAA CTTCGGATCT
    CTGTCTGCCC TGGTCTACCA GCACTCCATC ATCCCGCTGG CCCTGCCCTG CAAGCTGGTC
    ATTCCAAACC GAGACCCCAC AGATGAATCG AAAGATAGCT CGGGCCCTGC CAACTCAACC
    AGTGACCTGC TGAAGCAAGG GGCAGCCTGC AACGTGCTCT TCGTCAACTC TGTGGACATG
    GAGTCACTCA CTGGGCCACA GGCCATCTCC AAAGCCATTT CTGAGACCCT GGCTGCTGAC
    CCCACACCAG CTGCCACCAT CGTTCACTTC AAAGTTTCTG CCCAGGGAAT TACACTGACT
    GACAACCAGA GAAAGCTTTT CTTCAGGCGG CACTACCCCC TCAACACTGT GACCTTCTGT
    GACCTGGATC CACAGGAGAG GAAGTGGACA AAGACGGAGG GAGGTGCCCC TGCTAAGCTC
    TTCGGTTTTG TGGCCCGGAA ACAGGGCAGC GCCACGGACA ACGCCTGCCA CCTCTTTGCT
    GAGCTTGACC CCAACCAGCC GGCCTCCGCC ATCGTCAGCT TTGTCTCCAA GGTCATGCTG
    AGTGCCGGCC AGAAGAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227179
    Clone ID Related Accession (Same CDS sequence) XM_024999135.1
    Accession Version XM_024999135.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GTGCGTTCCC CTCCAGGTCT GGCCAAGACA CCCTTGTCTG CTCTGGGCCT GAAGCCCCAC
    AACCCAGCCG ACATCCTGCT GCATCCCACT GGTGTCCCCA GAAGACTGAT CCAGCCAGAG
    CCCCGGAGCT ACGTGGAGTC GGTGGTACGA ACGGCGGTGG CTGGGCCCCG GACTCAGGAA
    CCTGAGCCCA AGAGCTTTAG CGCCCCAGGT GCGCAGGCGT ATGGCCATGA GACACCCCTG
    AGGATTGGGA CCCTGGGAGG CTCCTTTGTC TCCCCTAGCC CCCTCTCCAC CAGCAGCCCC
    ATCCTCAGTG CTGACAGCAC TTCAGTGGGG AGTTTCCCAT CCGGGGAGAG CAGCGACCAG
    GGCGCGCGAA CACCTACCCA GCCTCTGCTG GATTCTGGCT TCCGCTCCGG CAGCCTGGGC
    CAGCCAAGTC CTTTGGCTCA GAGAAACTAC CAGAGCTCTT CTCCTCTCCC AACTGCAGGC
    AGCAGCTACA GCAGCCCCGA CTACTCACTG CAGCAGTTCA GCTCCCCAGA AGGCCAGGCC
    CGGTCTCAGT TCAGTGTGGC CGGTGTCCAC ACAGTGCCTG GGAGCCCCCA GGCACGGCAC
    AGGACAGTGG GCACCAATAC ACCCCCGAGC CCTGGCTTTG GCCGTCGGGC TGTGAACCCC
    AGCCTGGCTG CCCCCAGCAG CCCCAGTTTG AGCCACCGCC AGGTGATGGG TCCACTGGGA
    ACCGGTTTCC ATGGTAACAC GGGCTCCAGC CCCCAGAGCA GTGCAGCAAC CACTCCGGGA
    AGCCCCAGTC TGGGTCGCCA CCCTGGGGCC CACCAGGTTT CCAACCTCCA TGGCAATGTG
    GTCACCGCTC CTGGGAGCCC CAGCCTGGGC CGGCACCCCG GGGCCCACCA AGGCACCCTG
    GCCTCCAATC TCCATAGCAA TGCAGTAGCC AGCCCCGGAA GCCCCAGCCT GGGCCGTCAC
    TTGGGAGGGT CTGGATCTGT GGTTCCCGGC AGCCCCAGCT TGGACCGGCA CGTGCCCTAT
    GGTGGCTACT CCACCCCTGA GGACCGGAGA CCCACGCTGT CCCGGCAGAG CAGCGCCTCA
    GGCTACCAGG CTCCTTCCAC GCCCTCCTTC CCTGTGTCCC CCGCCTACTA CCCTGGCCTG
    AGCAGCCCCG CCACCTCCCC GTCGCCTGAT TCGGCAGCTT TCCGACAAGG GAGCCCCACC
    CCGGCCTTGC CGGAGAAGCG GAGGATGTCC ATGGGCGACC GAGCGGGCAG CCTCCCCAAC
    TACGCCACCG TCAACGGGAA GGTGTCCTCC TCGCCGGTCG CCAGTGGCAT GTCCAGTCCC
    AGCGGGGGCA GCACCGTCTC CTTCTCCCAC ACTCTGCCCG ACTTCTCCAA GTACTCCATG
    CCAGACAACA GTCCTGAGAC GCGGGCTAAA GTGAAGTTTG TCCAAGACAC TTCCAAGTAT
    TGGTACAAGC CTGAGATCTC CAGAGAACAG GCCATCGCCC TCCTTAAGGA CCAGGAGCCA
    GGGGCTTTCA TCATCCGTGA CAGCCACTCC TTCCGAGGAG CCTATGGGCT GGCCATGAAG
    GTGTCTTCGC CCCCTCCGAC CATCATGCAG CAGAATAAAA AAGGGGACAT GACCCACGAG
    CTCGTGAGGC ATTTCCTGAT AGAGACCGGC CCCAGAGGAG TCAAGCTCAA GGGCTGCCCC
    AATGAGCCAA ACTTCGGATC TCTGTCTGCC CTGGTCTACC AGCACTCCAT CATCCCGCTG
    GCCCTGCCCT GCAAGCTGGT CATTCCAAAC CGAGACCCCA CAGATGAATC GAAAGATAGC
    TCGGGCCCTG CCAACTCAAC CAGTGACCTG CTGAAGCAAG GGGCAGCCTG CAACGTGCTC
    TTCGTCAACT CTGTGGACAT GGAGTCACTC ACTGGGCCAC AGGCCATCTC CAAAGCCATT
    TCTGAGACCC TGGCTGCTGA CCCCACACCA GCTGCCACCA TCGTTCACTT CAAAGTTTCT
    GCCCAGGGAA TTACACTGAC TGACAACCAG AGAAAGCTTT TCTTCAGGCG GCACTACCCC
    CTCAACACTG TGACCTTCTG TGACCTGGAT CCACAGGAGA GGAAGTGGAC AAAGACGGAG
    GGAGGTGCCC CTGCTAAGCT CTTCGGTTTT GTGGCCCGGA AACAGGGCAG CGCCACGGAC
    AACGCCTGCC ACCTCTTTGC TGAGCTTGAC CCCAACCAGC CGGCCTCCGC CATCGTCAGC
    TTTGTCTCCA AGGTCATGCT GAGTGCCGGC CAGAAGAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854903.1
    CDS343..5244
    Translation

    Target ORF information:

    RefSeq Version XM_024999135.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999135.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GTGCGTTCCC CTCCAGGTCT GGCCAAGACA CCCTTGTCTG CTCTGGGCCT GAAGCCCCAC
    AACCCAGCCG ACATCCTGCT GCATCCCACT GGTGTCCCCA GAAGACTGAT CCAGCCAGAG
    CCCCGGAGCT ACGTGGAGTC GGTGGTACGA ACGGCGGTGG CTGGGCCCCG GACTCAGGAA
    CCTGAGCCCA AGAGCTTTAG CGCCCCAGGT GCGCAGGCGT ATGGCCATGA GACACCCCTG
    AGGATTGGGA CCCTGGGAGG CTCCTTTGTC TCCCCTAGCC CCCTCTCCAC CAGCAGCCCC
    ATCCTCAGTG CTGACAGCAC TTCAGTGGGG AGTTTCCCAT CCGGGGAGAG CAGCGACCAG
    GGCGCGCGAA CACCTACCCA GCCTCTGCTG GATTCTGGCT TCCGCTCCGG CAGCCTGGGC
    CAGCCAAGTC CTTTGGCTCA GAGAAACTAC CAGAGCTCTT CTCCTCTCCC AACTGCAGGC
    AGCAGCTACA GCAGCCCCGA CTACTCACTG CAGCAGTTCA GCTCCCCAGA AGGCCAGGCC
    CGGTCTCAGT TCAGTGTGGC CGGTGTCCAC ACAGTGCCTG GGAGCCCCCA GGCACGGCAC
    AGGACAGTGG GCACCAATAC ACCCCCGAGC CCTGGCTTTG GCCGTCGGGC TGTGAACCCC
    AGCCTGGCTG CCCCCAGCAG CCCCAGTTTG AGCCACCGCC AGGTGATGGG TCCACTGGGA
    ACCGGTTTCC ATGGTAACAC GGGCTCCAGC CCCCAGAGCA GTGCAGCAAC CACTCCGGGA
    AGCCCCAGTC TGGGTCGCCA CCCTGGGGCC CACCAGGTTT CCAACCTCCA TGGCAATGTG
    GTCACCGCTC CTGGGAGCCC CAGCCTGGGC CGGCACCCCG GGGCCCACCA AGGCACCCTG
    GCCTCCAATC TCCATAGCAA TGCAGTAGCC AGCCCCGGAA GCCCCAGCCT GGGCCGTCAC
    TTGGGAGGGT CTGGATCTGT GGTTCCCGGC AGCCCCAGCT TGGACCGGCA CGTGCCCTAT
    GGTGGCTACT CCACCCCTGA GGACCGGAGA CCCACGCTGT CCCGGCAGAG CAGCGCCTCA
    GGCTACCAGG CTCCTTCCAC GCCCTCCTTC CCTGTGTCCC CCGCCTACTA CCCTGGCCTG
    AGCAGCCCCG CCACCTCCCC GTCGCCTGAT TCGGCAGCTT TCCGACAAGG GAGCCCCACC
    CCGGCCTTGC CGGAGAAGCG GAGGATGTCC ATGGGCGACC GAGCGGGCAG CCTCCCCAAC
    TACGCCACCG TCAACGGGAA GGTGTCCTCC TCGCCGGTCG CCAGTGGCAT GTCCAGTCCC
    AGCGGGGGCA GCACCGTCTC CTTCTCCCAC ACTCTGCCCG ACTTCTCCAA GTACTCCATG
    CCAGACAACA GTCCTGAGAC GCGGGCTAAA GTGAAGTTTG TCCAAGACAC TTCCAAGTAT
    TGGTACAAGC CTGAGATCTC CAGAGAACAG GCCATCGCCC TCCTTAAGGA CCAGGAGCCA
    GGGGCTTTCA TCATCCGTGA CAGCCACTCC TTCCGAGGAG CCTATGGGCT GGCCATGAAG
    GTGTCTTCGC CCCCTCCGAC CATCATGCAG CAGAATAAAA AAGGGGACAT GACCCACGAG
    CTCGTGAGGC ATTTCCTGAT AGAGACCGGC CCCAGAGGAG TCAAGCTCAA GGGCTGCCCC
    AATGAGCCAA ACTTCGGATC TCTGTCTGCC CTGGTCTACC AGCACTCCAT CATCCCGCTG
    GCCCTGCCCT GCAAGCTGGT CATTCCAAAC CGAGACCCCA CAGATGAATC GAAAGATAGC
    TCGGGCCCTG CCAACTCAAC CAGTGACCTG CTGAAGCAAG GGGCAGCCTG CAACGTGCTC
    TTCGTCAACT CTGTGGACAT GGAGTCACTC ACTGGGCCAC AGGCCATCTC CAAAGCCATT
    TCTGAGACCC TGGCTGCTGA CCCCACACCA GCTGCCACCA TCGTTCACTT CAAAGTTTCT
    GCCCAGGGAA TTACACTGAC TGACAACCAG AGAAAGCTTT TCTTCAGGCG GCACTACCCC
    CTCAACACTG TGACCTTCTG TGACCTGGAT CCACAGGAGA GGAAGTGGAC AAAGACGGAG
    GGAGGTGCCC CTGCTAAGCT CTTCGGTTTT GTGGCCCGGA AACAGGGCAG CGCCACGGAC
    AACGCCTGCC ACCTCTTTGC TGAGCTTGAC CCCAACCAGC CGGCCTCCGC CATCGTCAGC
    TTTGTCTCCA AGGTCATGCT GAGTGCCGGC CAGAAGAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227180
    Clone ID Related Accession (Same CDS sequence) XM_024999138.1
    Accession Version XM_024999138.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4878bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GTGCGTTCCC CTCCAGGTCT GGCCAAGACA CCCTTGTCTG CTCTGGGCCT GAAGCCCCAC
    AACCCAGCCG ACATCCTGCT GCATCCCACT GGTGAGCCCC GGAGCTACGT GGAGTCGGTG
    GTACGAACGG CGGTGGCTGG GCCCCGGACT CAGGAACCTG AGCCCAAGAG CTTTAGCGCC
    CCAGGTGCGC AGGCGTATGG CCATGAGACA CCCCTGAGGA TTGGGACCCT GGGAGGCTCC
    TTTGTCTCCC CTAGCCCCCT CTCCACCAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCA
    GTGGGGAGTT TCCCATCCGG GGAGAGCAGC GACCAGGGCG CGCGAACACC TACCCAGCCT
    CTGCTGGATT CTGGCTTCCG CTCCGGCAGC CTGGGCCAGC CAAGTCCTTT GGCTCAGAGA
    AACTACCAGA GCTCTTCTCC TCTCCCAACT GCAGGCAGCA GCTACAGCAG CCCCGACTAC
    TCACTGCAGC AGTTCAGCTC CCCAGAAGGC CAGGCCCGGT CTCAGTTCAG TGTGGCCGGT
    GTCCACACAG TGCCTGGGAG CCCCCAGGCA CGGCACAGGA CAGTGGGCAC CAATACACCC
    CCGAGCCCTG GCTTTGGCCG TCGGGCTGTG AACCCCAGCC TGGCTGCCCC CAGCAGCCCC
    AGTTTGAGCC ACCGCCAGGT GATGGGTCCA CTGGGAACCG GTTTCCATGG TAACACGGGC
    TCCAGCCCCC AGAGCAGTGC AGCAACCACT CCGGGAAGCC CCAGTCTGGG TCGCCACCCT
    GGGGCCCACC AGGTTTCCAA CCTCCATGGC AATGTGGTCA CCGCTCCTGG GAGCCCCAGC
    CTGGGCCGGC ACCCCGGGGC CCACCAAGGC ACCCTGGCCT CCAATCTCCA TAGCAATGCA
    GTAGCCAGCC CCGGAAGCCC CAGCCTGGGC CGTCACTTGG GAGGGTCTGG ATCTGTGGTT
    CCCGGCAGCC CCAGCTTGGA CCGGCACGTG CCCTATGGTG GCTACTCCAC CCCTGAGGAC
    CGGAGACCCA CGCTGTCCCG GCAGAGCAGC GCCTCAGGCT ACCAGGCTCC TTCCACGCCC
    TCCTTCCCTG TGTCCCCCGC CTACTACCCT GGCCTGAGCA GCCCCGCCAC CTCCCCGTCG
    CCTGATTCGG CAGCTTTCCG ACAAGGGAGC CCCACCCCGG CCTTGCCGGA GAAGCGGAGG
    ATGTCCATGG GCGACCGAGC GGGCAGCCTC CCCAACTACG CCACCGTCAA CGGGAAGGTG
    TCCTCCTCGC CGGTCGCCAG TGGCATGTCC AGTCCCAGCG GGGGCAGCAC CGTCTCCTTC
    TCCCACACTC TGCCCGACTT CTCCAAGTAC TCCATGCCAG ACAACAGTCC TGAGACGCGG
    GCTAAAGTGA AGTTTGTCCA AGACACTTCC AAGTATTGGT ACAAGCCTGA GATCTCCAGA
    GAACAGGCCA TCGCCCTCCT TAAGGACCAG GAGCCAGGGG CTTTCATCAT CCGTGACAGC
    CACTCCTTCC GAGGAGCCTA TGGGCTGGCC ATGAAGGTGT CTTCGCCCCC TCCGACCATC
    ATGCAGCAGA ATAAAAAAGG GGACATGACC CACGAGCTCG TGAGGCATTT CCTGATAGAG
    ACCGGCCCCA GAGGAGTCAA GCTCAAGGGC TGCCCCAATG AGCCAAACTT CGGATCTCTG
    TCTGCCCTGG TCTACCAGCA CTCCATCATC CCGCTGGCCC TGCCCTGCAA GCTGGTCATT
    CCAAACCGAG ACCCCACAGA TGAATCGAAA GATAGCTCGG GCCCTGCCAA CTCAACCAGT
    GACCTGCTGA AGCAAGGGGC AGCCTGCAAC GTGCTCTTCG TCAACTCTGT GGACATGGAG
    TCACTCACTG GGCCACAGGC CATCTCCAAA GCCATTTCTG AGACCCTGGC TGCTGACCCC
    ACACCAGCTG CCACCATCGT TCACTTCAAA GTTTCTGCCC AGGGAATTAC ACTGACTGAC
    AACCAGAGAA AGCTTTTCTT CAGGCGGCAC TACCCCCTCA ACACTGTGAC CTTCTGTGAC
    CTGGATCCAC AGGAGAGGAA GTGGACAAAG ACGGAGGGAG GTGCCCCTGC TAAGCTCTTC
    GGTTTTGTGG CCCGGAAACA GGGCAGCGCC ACGGACAACG CCTGCCACCT CTTTGCTGAG
    CTTGACCCCA ACCAGCCGGC CTCCGCCATC GTCAGCTTTG TCTCCAAGGT CATGCTGAGT
    GCCGGCCAGA AGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854906.1
    CDS343..5220
    Translation

    Target ORF information:

    RefSeq Version XM_024999138.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999138.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GTGCGTTCCC CTCCAGGTCT GGCCAAGACA CCCTTGTCTG CTCTGGGCCT GAAGCCCCAC
    AACCCAGCCG ACATCCTGCT GCATCCCACT GGTGAGCCCC GGAGCTACGT GGAGTCGGTG
    GTACGAACGG CGGTGGCTGG GCCCCGGACT CAGGAACCTG AGCCCAAGAG CTTTAGCGCC
    CCAGGTGCGC AGGCGTATGG CCATGAGACA CCCCTGAGGA TTGGGACCCT GGGAGGCTCC
    TTTGTCTCCC CTAGCCCCCT CTCCACCAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCA
    GTGGGGAGTT TCCCATCCGG GGAGAGCAGC GACCAGGGCG CGCGAACACC TACCCAGCCT
    CTGCTGGATT CTGGCTTCCG CTCCGGCAGC CTGGGCCAGC CAAGTCCTTT GGCTCAGAGA
    AACTACCAGA GCTCTTCTCC TCTCCCAACT GCAGGCAGCA GCTACAGCAG CCCCGACTAC
    TCACTGCAGC AGTTCAGCTC CCCAGAAGGC CAGGCCCGGT CTCAGTTCAG TGTGGCCGGT
    GTCCACACAG TGCCTGGGAG CCCCCAGGCA CGGCACAGGA CAGTGGGCAC CAATACACCC
    CCGAGCCCTG GCTTTGGCCG TCGGGCTGTG AACCCCAGCC TGGCTGCCCC CAGCAGCCCC
    AGTTTGAGCC ACCGCCAGGT GATGGGTCCA CTGGGAACCG GTTTCCATGG TAACACGGGC
    TCCAGCCCCC AGAGCAGTGC AGCAACCACT CCGGGAAGCC CCAGTCTGGG TCGCCACCCT
    GGGGCCCACC AGGTTTCCAA CCTCCATGGC AATGTGGTCA CCGCTCCTGG GAGCCCCAGC
    CTGGGCCGGC ACCCCGGGGC CCACCAAGGC ACCCTGGCCT CCAATCTCCA TAGCAATGCA
    GTAGCCAGCC CCGGAAGCCC CAGCCTGGGC CGTCACTTGG GAGGGTCTGG ATCTGTGGTT
    CCCGGCAGCC CCAGCTTGGA CCGGCACGTG CCCTATGGTG GCTACTCCAC CCCTGAGGAC
    CGGAGACCCA CGCTGTCCCG GCAGAGCAGC GCCTCAGGCT ACCAGGCTCC TTCCACGCCC
    TCCTTCCCTG TGTCCCCCGC CTACTACCCT GGCCTGAGCA GCCCCGCCAC CTCCCCGTCG
    CCTGATTCGG CAGCTTTCCG ACAAGGGAGC CCCACCCCGG CCTTGCCGGA GAAGCGGAGG
    ATGTCCATGG GCGACCGAGC GGGCAGCCTC CCCAACTACG CCACCGTCAA CGGGAAGGTG
    TCCTCCTCGC CGGTCGCCAG TGGCATGTCC AGTCCCAGCG GGGGCAGCAC CGTCTCCTTC
    TCCCACACTC TGCCCGACTT CTCCAAGTAC TCCATGCCAG ACAACAGTCC TGAGACGCGG
    GCTAAAGTGA AGTTTGTCCA AGACACTTCC AAGTATTGGT ACAAGCCTGA GATCTCCAGA
    GAACAGGCCA TCGCCCTCCT TAAGGACCAG GAGCCAGGGG CTTTCATCAT CCGTGACAGC
    CACTCCTTCC GAGGAGCCTA TGGGCTGGCC ATGAAGGTGT CTTCGCCCCC TCCGACCATC
    ATGCAGCAGA ATAAAAAAGG GGACATGACC CACGAGCTCG TGAGGCATTT CCTGATAGAG
    ACCGGCCCCA GAGGAGTCAA GCTCAAGGGC TGCCCCAATG AGCCAAACTT CGGATCTCTG
    TCTGCCCTGG TCTACCAGCA CTCCATCATC CCGCTGGCCC TGCCCTGCAA GCTGGTCATT
    CCAAACCGAG ACCCCACAGA TGAATCGAAA GATAGCTCGG GCCCTGCCAA CTCAACCAGT
    GACCTGCTGA AGCAAGGGGC AGCCTGCAAC GTGCTCTTCG TCAACTCTGT GGACATGGAG
    TCACTCACTG GGCCACAGGC CATCTCCAAA GCCATTTCTG AGACCCTGGC TGCTGACCCC
    ACACCAGCTG CCACCATCGT TCACTTCAAA GTTTCTGCCC AGGGAATTAC ACTGACTGAC
    AACCAGAGAA AGCTTTTCTT CAGGCGGCAC TACCCCCTCA ACACTGTGAC CTTCTGTGAC
    CTGGATCCAC AGGAGAGGAA GTGGACAAAG ACGGAGGGAG GTGCCCCTGC TAAGCTCTTC
    GGTTTTGTGG CCCGGAAACA GGGCAGCGCC ACGGACAACG CCTGCCACCT CTTTGCTGAG
    CTTGACCCCA ACCAGCCGGC CTCCGCCATC GTCAGCTTTG TCTCCAAGGT CATGCTGAGT
    GCCGGCCAGA AGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227181
    Clone ID Related Accession (Same CDS sequence) XM_024999142.1
    Accession Version XM_024999142.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GCGTACAATG CCAGGCTGCA GGGCACTGGG CGGAGAGTCA GCTCCTGGCA CCCTCCTCTC
    CACCGGCTCC GATCTTCCTC TCTCTCTGGG GTGCAGGCTC GGGAGAAGCA GCCTGCAGAG
    CCCCCAGCAC CTCTCCGGAA GCGGGCTGCC AGTGATGGGC AGTATGAGAA CCAGTCACCA
    GAACCCACAT CCCCCCGTAG CCCTGGGGTT CGCTCGCCTG TCCAATGTGT CTCCCCAGAG
    CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGACCCA AAGAGCCCCA TCTGCACAGC
    TACAAGGAAG CCTTTGAGGA GATGGAGGGA ACCTCTCCGA CCAGCCCACC ACCCAGTGGG
    GTGCGTTCCC CTCCAGGTCT GGCCAAGACA CCCTTGTCTG CTCTGGGCCT GAAGCCCCAC
    AACCCAGCCG ACATCCTGCT GCATCCCACT GGTGTCCCCA GAAGACTGAT CCAGCCAGAG
    GAGGATGAGA GGAAGGTGCT GACAGAGCTT TCTGAAGAGC CCCGGAGCTA CGTGGAGTCG
    GTGGTACGAA CGGCGGTGGC TGGGCCCCGG ACTCAGGAAC CTGAGCCCAA GAGCTTTAGC
    GCCCCAGGTG CGCAGGCGTA TGGCCATGAG ACACCCCTGA GGATTGGGAC CCTGGGAGGC
    TCCTTTGTCT CCCCTAGCCC CCTCTCCACC AGCAGCCCCA TCCTCAGTGC TGACAGCACT
    TCAGTGGGGA GTTTCCCATC CGGGGAGAGC AGCGACCAGG GCGCGCGAAC ACCTACCCAG
    CCTCTGCTGG ATTCTGGCTT CCGCTCCGGC AGCCTGGGCC AGCCAAGTCC TTTGGCTCAG
    AGAAACTACC AGAGCTCTTC TCCTCTCCCA ACTGCAGGCA GCAGCTACAG CAGCCCCGAC
    TACTCACTGC AGCAGTTCAG CTCCCCAGAA GGCCAGGCCC GGTCTCAGTT CAGTGTGGCC
    GGTGTCCACA CAGTGCCTGG GAGCCCCCAG GCACGGCACA GGACAGTGGG CACCAATACA
    CCCCCGAGCC CTGGCTTTGG CCGTCGGGCT GTGAACCCCA GCCTGGCTGC CCCCAGCAGC
    CCCAGTTTGA GCCACCGCCA GGTGATGGGT CCACTGGGAA CCGGTTTCCA TGGTAACACG
    GGCTCCAGCC CCCAGAGCAG TGCAGCAACC ACTCCGGGAA GCCCCAGTCT GGGTCGCCAC
    CCTGGGGCCC ACCAGGTTTC CAACCTCCAT GGCAATGTGG TCACCGCTCC TGGGAGCCCC
    AGCCTGGGCC GGCACCCCGG GGCCCACCAA GGCACCCTGG CCTCCAATCT CCATAGCAAT
    GCAGTAGCCA GCCCCGGAAG CCCCAGCCTG GGCCGTCACT TGGGAGGGTC TGGATCTGTG
    GTTCCCGGCA GCCCCAGCTT GGACCGGCAC GTGCCCTATG GTGGCTACTC CACCCCTGAG
    GACCGGAGAC CCACGCTGTC CCGGCAGAGC AGCGCCTCAG GCTACCAGGC TCCTTCCACG
    CCCTCCTTCC CTGTGTCCCC CGCCTACTAC CCTGGCCTGA GCAGCCCCGC CACCTCCCCG
    TCGCCTGATT CGGCAGCTTT CCGACAAGGG AGCCCCACCC CGGCCTTGCC GGAGAAGCGG
    AGGATGTCCA TGGGCGACCG AGCGGGCAGC CTCCCCAACT ACGCCACCGT CAACGGGAAG
    GTGTCCTCCT CGCCGGTCGC CAGTGGCATG TCCAGTCCCA GCGGGGGCAG CACCGTCTCC
    TTCTCCCACA CTCTGCCCGA CTTCTCCAAG TACTCCATGC CAGACAACAG TCCTGAGACG
    CGGGCTAAAG TGAAGTTTGT CCAAGACACT TCCAAGTATT GGTACAAGCC TGAGATCTCC
    AGAGAACAGG CCATCGCCCT CCTTAAGGAC CAGGAGCCAG GGGCTTTCAT CATCCGTGAC
    AGCCACTCCT TCCGAGGAGC CTATGGGCTG GCCATGAAGG TGTCTTCGCC CCCTCCGACC
    ATCATGCAGC AGAATAAAAA AGGGGACATG ACCCACGAGC TCGTGAGGCA TTTCCTGATA
    GAGACCGGCC CCAGAGGAGT CAAGCTCAAG GGCTGCCCCA ATGAGCCAAA CTTCGGATCT
    CTGTCTGCCC TGGTCTACCA GCACTCCATC ATCCCGCTGG CCCTGCCCTG CAAGCTGGTC
    ATTCCAAACC GAGACCCCAC AGATGAATCG AAAGATAGCT CGGGCCCTGC CAACTCAACC
    AGTGACCTGC TGAAGCAAGG GGCAGCCTGC AACGTGCTCT TCGTCAACTC TGTGGACATG
    GAGTCACTCA CTGGGCCACA GGCCATCTCC AAAGCCATTT CTGAGACCCT GGCTGCTGAC
    CCCACACCAG CTGCCACCAT CGTTCACTTC AAAGTTTCTG CCCAGGGAAT TACACTGACT
    GACAACCAGA GAAAGCTTTT CTTCAGGCGG CACTACCCCC TCAACACTGT GACCTTCTGT
    GACCTGGATC CACAGGAGAG GAAGTGGACA AAGACGGAGG GAGGTGCCCC TGCTAAGCTC
    TTCGGTTTTG TGGCCCGGAA ACAGGGCAGC GCCACGGACA ACGCCTGCCA CCTCTTTGCT
    GAGCTTGACC CCAACCAGCC GGCCTCCGCC ATCGTCAGCT TTGTCTCCAA GGTCATGCTG
    AGTGCCGGCC AGAAGAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854910.1
    CDS412..5712
    Translation

    Target ORF information:

    RefSeq Version XM_024999142.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999142.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GCGTACAATG CCAGGCTGCA GGGCACTGGG CGGAGAGTCA GCTCCTGGCA CCCTCCTCTC
    CACCGGCTCC GATCTTCCTC TCTCTCTGGG GTGCAGGCTC GGGAGAAGCA GCCTGCAGAG
    CCCCCAGCAC CTCTCCGGAA GCGGGCTGCC AGTGATGGGC AGTATGAGAA CCAGTCACCA
    GAACCCACAT CCCCCCGTAG CCCTGGGGTT CGCTCGCCTG TCCAATGTGT CTCCCCAGAG
    CTGGCTCTTA CCATCGCTCT CAATCCTGGA GGGCGACCCA AAGAGCCCCA TCTGCACAGC
    TACAAGGAAG CCTTTGAGGA GATGGAGGGA ACCTCTCCGA CCAGCCCACC ACCCAGTGGG
    GTGCGTTCCC CTCCAGGTCT GGCCAAGACA CCCTTGTCTG CTCTGGGCCT GAAGCCCCAC
    AACCCAGCCG ACATCCTGCT GCATCCCACT GGTGTCCCCA GAAGACTGAT CCAGCCAGAG
    GAGGATGAGA GGAAGGTGCT GACAGAGCTT TCTGAAGAGC CCCGGAGCTA CGTGGAGTCG
    GTGGTACGAA CGGCGGTGGC TGGGCCCCGG ACTCAGGAAC CTGAGCCCAA GAGCTTTAGC
    GCCCCAGGTG CGCAGGCGTA TGGCCATGAG ACACCCCTGA GGATTGGGAC CCTGGGAGGC
    TCCTTTGTCT CCCCTAGCCC CCTCTCCACC AGCAGCCCCA TCCTCAGTGC TGACAGCACT
    TCAGTGGGGA GTTTCCCATC CGGGGAGAGC AGCGACCAGG GCGCGCGAAC ACCTACCCAG
    CCTCTGCTGG ATTCTGGCTT CCGCTCCGGC AGCCTGGGCC AGCCAAGTCC TTTGGCTCAG
    AGAAACTACC AGAGCTCTTC TCCTCTCCCA ACTGCAGGCA GCAGCTACAG CAGCCCCGAC
    TACTCACTGC AGCAGTTCAG CTCCCCAGAA GGCCAGGCCC GGTCTCAGTT CAGTGTGGCC
    GGTGTCCACA CAGTGCCTGG GAGCCCCCAG GCACGGCACA GGACAGTGGG CACCAATACA
    CCCCCGAGCC CTGGCTTTGG CCGTCGGGCT GTGAACCCCA GCCTGGCTGC CCCCAGCAGC
    CCCAGTTTGA GCCACCGCCA GGTGATGGGT CCACTGGGAA CCGGTTTCCA TGGTAACACG
    GGCTCCAGCC CCCAGAGCAG TGCAGCAACC ACTCCGGGAA GCCCCAGTCT GGGTCGCCAC
    CCTGGGGCCC ACCAGGTTTC CAACCTCCAT GGCAATGTGG TCACCGCTCC TGGGAGCCCC
    AGCCTGGGCC GGCACCCCGG GGCCCACCAA GGCACCCTGG CCTCCAATCT CCATAGCAAT
    GCAGTAGCCA GCCCCGGAAG CCCCAGCCTG GGCCGTCACT TGGGAGGGTC TGGATCTGTG
    GTTCCCGGCA GCCCCAGCTT GGACCGGCAC GTGCCCTATG GTGGCTACTC CACCCCTGAG
    GACCGGAGAC CCACGCTGTC CCGGCAGAGC AGCGCCTCAG GCTACCAGGC TCCTTCCACG
    CCCTCCTTCC CTGTGTCCCC CGCCTACTAC CCTGGCCTGA GCAGCCCCGC CACCTCCCCG
    TCGCCTGATT CGGCAGCTTT CCGACAAGGG AGCCCCACCC CGGCCTTGCC GGAGAAGCGG
    AGGATGTCCA TGGGCGACCG AGCGGGCAGC CTCCCCAACT ACGCCACCGT CAACGGGAAG
    GTGTCCTCCT CGCCGGTCGC CAGTGGCATG TCCAGTCCCA GCGGGGGCAG CACCGTCTCC
    TTCTCCCACA CTCTGCCCGA CTTCTCCAAG TACTCCATGC CAGACAACAG TCCTGAGACG
    CGGGCTAAAG TGAAGTTTGT CCAAGACACT TCCAAGTATT GGTACAAGCC TGAGATCTCC
    AGAGAACAGG CCATCGCCCT CCTTAAGGAC CAGGAGCCAG GGGCTTTCAT CATCCGTGAC
    AGCCACTCCT TCCGAGGAGC CTATGGGCTG GCCATGAAGG TGTCTTCGCC CCCTCCGACC
    ATCATGCAGC AGAATAAAAA AGGGGACATG ACCCACGAGC TCGTGAGGCA TTTCCTGATA
    GAGACCGGCC CCAGAGGAGT CAAGCTCAAG GGCTGCCCCA ATGAGCCAAA CTTCGGATCT
    CTGTCTGCCC TGGTCTACCA GCACTCCATC ATCCCGCTGG CCCTGCCCTG CAAGCTGGTC
    ATTCCAAACC GAGACCCCAC AGATGAATCG AAAGATAGCT CGGGCCCTGC CAACTCAACC
    AGTGACCTGC TGAAGCAAGG GGCAGCCTGC AACGTGCTCT TCGTCAACTC TGTGGACATG
    GAGTCACTCA CTGGGCCACA GGCCATCTCC AAAGCCATTT CTGAGACCCT GGCTGCTGAC
    CCCACACCAG CTGCCACCAT CGTTCACTTC AAAGTTTCTG CCCAGGGAAT TACACTGACT
    GACAACCAGA GAAAGCTTTT CTTCAGGCGG CACTACCCCC TCAACACTGT GACCTTCTGT
    GACCTGGATC CACAGGAGAG GAAGTGGACA AAGACGGAGG GAGGTGCCCC TGCTAAGCTC
    TTCGGTTTTG TGGCCCGGAA ACAGGGCAGC GCCACGGACA ACGCCTGCCA CCTCTTTGCT
    GAGCTTGACC CCAACCAGCC GGCCTCCGCC ATCGTCAGCT TTGTCTCCAA GGTCATGCTG
    AGTGCCGGCC AGAAGAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227182
    Clone ID Related Accession (Same CDS sequence) XM_024999153.1
    Accession Version XM_024999153.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tensin-1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GAGCCCCGGA GCTACGTGGA GTCGGTGGTA CGAACGGCGG TGGCTGGGCC CCGGACTCAG
    GAACCTGAGC CCAAGAGCTT TAGCGCCCCA GGTGCGCAGG CGTATGGCCA TGAGACACCC
    CTGAGGATTG GGACCCTGGG AGGCTCCTTT GTCTCCCCTA GCCCCCTCTC CACCAGCAGC
    CCCATCCTCA GTGCTGACAG CACTTCAGTG GGGAGTTTCC CATCCGGGGA GAGCAGCGAC
    CAGGGCGCGC GAACACCTAC CCAGCCTCTG CTGGATTCTG GCTTCCGCTC CGGCAGCCTG
    GGCCAGCCAA GTCCTTTGGC TCAGAGAAAC TACCAGAGCT CTTCTCCTCT CCCAACTGCA
    GGCAGCAGCT ACAGCAGCCC CGACTACTCA CTGCAGCAGT TCAGCTCCCC AGAAGGCCAG
    GCCCGGTCTC AGTTCAGTGT GGCCGGTGTC CACACAGTGC CTGGGAGCCC CCAGGCACGG
    CACAGGACAG TGGGCACCAA TACACCCCCG AGCCCTGGCT TTGGCCGTCG GGCTGTGAAC
    CCCAGCCTGG CTGCCCCCAG CAGCCCCAGT TTGAGCCACC GCCAGGTGAT GGGTCCACTG
    GGAACCGGTT TCCATGGTAA CACGGGCTCC AGCCCCCAGA GCAGTGCAGC AACCACTCCG
    GGAAGCCCCA GTCTGGGTCG CCACCCTGGG GCCCACCAGG TTTCCAACCT CCATGGCAAT
    GTGGTCACCG CTCCTGGGAG CCCCAGCCTG GGCCGGCACC CCGGGGCCCA CCAAGGCACC
    CTGGCCTCCA ATCTCCATAG CAATGCAGTA GCCAGCCCCG GAAGCCCCAG CCTGGGCCGT
    CACTTGGGAG GGTCTGGATC TGTGGTTCCC GGCAGCCCCA GCTTGGACCG GCACGTGCCC
    TATGGTGGCT ACTCCACCCC TGAGGACCGG AGACCCACGC TGTCCCGGCA GAGCAGCGCC
    TCAGGCTACC AGGCTCCTTC CACGCCCTCC TTCCCTGTGT CCCCCGCCTA CTACCCTGGC
    CTGAGCAGCC CCGCCACCTC CCCGTCGCCT GATTCGGCAG CTTTCCGACA AGGGAGCCCC
    ACCCCGGCCT TGCCGGAGAA GCGGAGGATG TCCATGGGCG ACCGAGCGGG CAGCCTCCCC
    AACTACGCCA CCGTCAACGG GAAGGTGTCC TCCTCGCCGG TCGCCAGTGG CATGTCCAGT
    CCCAGCGGGG GCAGCACCGT CTCCTTCTCC CACACTCTGC CCGACTTCTC CAAGTACTCC
    ATGCCAGACA ACAGTCCTGA GACGCGGGCT AAAGTGAAGT TTGTCCAAGA CACTTCCAAG
    TATTGGTACA AGCCTGAGAT CTCCAGAGAA CAGGCCATCG CCCTCCTTAA GGACCAGGAG
    CCAGGGGCTT TCATCATCCG TGACAGCCAC TCCTTCCGAG GAGCCTATGG GCTGGCCATG
    AAGGTGTCTT CGCCCCCTCC GACCATCATG CAGCAGAATA AAAAAGGGGA CATGACCCAC
    GAGCTCGTGA GGCATTTCCT GATAGAGACC GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC
    CCCAATGAGC CAAACTTCGG ATCTCTGTCT GCCCTGGTCT ACCAGCACTC CATCATCCCG
    CTGGCCCTGC CCTGCAAGCT GGTCATTCCA AACCGAGACC CCACAGATGA ATCGAAAGAT
    AGCTCGGGCC CTGCCAACTC AACCAGTGAC CTGCTGAAGC AAGGGGCAGC CTGCAACGTG
    CTCTTCGTCA ACTCTGTGGA CATGGAGTCA CTCACTGGGC CACAGGCCAT CTCCAAAGCC
    ATTTCTGAGA CCCTGGCTGC TGACCCCACA CCAGCTGCCA CCATCGTTCA CTTCAAAGTT
    TCTGCCCAGG GAATTACACT GACTGACAAC CAGAGAAAGC TTTTCTTCAG GCGGCACTAC
    CCCCTCAACA CTGTGACCTT CTGTGACCTG GATCCACAGG AGAGGAAGTG GACAAAGACG
    GAGGGAGGTG CCCCTGCTAA GCTCTTCGGT TTTGTGGCCC GGAAACAGGG CAGCGCCACG
    GACAACGCCT GCCACCTCTT TGCTGAGCTT GACCCCAACC AGCCGGCCTC CGCCATCGTC
    AGCTTTGTCT CCAAGGTCAT GCTGAGTGCC GGCCAGAAGA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854921.1
    CDS343..5127
    Translation

    Target ORF information:

    RefSeq Version XM_024999153.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999153.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCAGGCC
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGGA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCAGGGACCC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCACTGGG
    GCTGTCAACG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GCCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGCCTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGCGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGCCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAACGGTGGG GCCTTAGCGT CCGAGCGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GAGCCCCGGA GCTACGTGGA GTCGGTGGTA CGAACGGCGG TGGCTGGGCC CCGGACTCAG
    GAACCTGAGC CCAAGAGCTT TAGCGCCCCA GGTGCGCAGG CGTATGGCCA TGAGACACCC
    CTGAGGATTG GGACCCTGGG AGGCTCCTTT GTCTCCCCTA GCCCCCTCTC CACCAGCAGC
    CCCATCCTCA GTGCTGACAG CACTTCAGTG GGGAGTTTCC CATCCGGGGA GAGCAGCGAC
    CAGGGCGCGC GAACACCTAC CCAGCCTCTG CTGGATTCTG GCTTCCGCTC CGGCAGCCTG
    GGCCAGCCAA GTCCTTTGGC TCAGAGAAAC TACCAGAGCT CTTCTCCTCT CCCAACTGCA
    GGCAGCAGCT ACAGCAGCCC CGACTACTCA CTGCAGCAGT TCAGCTCCCC AGAAGGCCAG
    GCCCGGTCTC AGTTCAGTGT GGCCGGTGTC CACACAGTGC CTGGGAGCCC CCAGGCACGG
    CACAGGACAG TGGGCACCAA TACACCCCCG AGCCCTGGCT TTGGCCGTCG GGCTGTGAAC
    CCCAGCCTGG CTGCCCCCAG CAGCCCCAGT TTGAGCCACC GCCAGGTGAT GGGTCCACTG
    GGAACCGGTT TCCATGGTAA CACGGGCTCC AGCCCCCAGA GCAGTGCAGC AACCACTCCG
    GGAAGCCCCA GTCTGGGTCG CCACCCTGGG GCCCACCAGG TTTCCAACCT CCATGGCAAT
    GTGGTCACCG CTCCTGGGAG CCCCAGCCTG GGCCGGCACC CCGGGGCCCA CCAAGGCACC
    CTGGCCTCCA ATCTCCATAG CAATGCAGTA GCCAGCCCCG GAAGCCCCAG CCTGGGCCGT
    CACTTGGGAG GGTCTGGATC TGTGGTTCCC GGCAGCCCCA GCTTGGACCG GCACGTGCCC
    TATGGTGGCT ACTCCACCCC TGAGGACCGG AGACCCACGC TGTCCCGGCA GAGCAGCGCC
    TCAGGCTACC AGGCTCCTTC CACGCCCTCC TTCCCTGTGT CCCCCGCCTA CTACCCTGGC
    CTGAGCAGCC CCGCCACCTC CCCGTCGCCT GATTCGGCAG CTTTCCGACA AGGGAGCCCC
    ACCCCGGCCT TGCCGGAGAA GCGGAGGATG TCCATGGGCG ACCGAGCGGG CAGCCTCCCC
    AACTACGCCA CCGTCAACGG GAAGGTGTCC TCCTCGCCGG TCGCCAGTGG CATGTCCAGT
    CCCAGCGGGG GCAGCACCGT CTCCTTCTCC CACACTCTGC CCGACTTCTC CAAGTACTCC
    ATGCCAGACA ACAGTCCTGA GACGCGGGCT AAAGTGAAGT TTGTCCAAGA CACTTCCAAG
    TATTGGTACA AGCCTGAGAT CTCCAGAGAA CAGGCCATCG CCCTCCTTAA GGACCAGGAG
    CCAGGGGCTT TCATCATCCG TGACAGCCAC TCCTTCCGAG GAGCCTATGG GCTGGCCATG
    AAGGTGTCTT CGCCCCCTCC GACCATCATG CAGCAGAATA AAAAAGGGGA CATGACCCAC
    GAGCTCGTGA GGCATTTCCT GATAGAGACC GGCCCCAGAG GAGTCAAGCT CAAGGGCTGC
    CCCAATGAGC CAAACTTCGG ATCTCTGTCT GCCCTGGTCT ACCAGCACTC CATCATCCCG
    CTGGCCCTGC CCTGCAAGCT GGTCATTCCA AACCGAGACC CCACAGATGA ATCGAAAGAT
    AGCTCGGGCC CTGCCAACTC AACCAGTGAC CTGCTGAAGC AAGGGGCAGC CTGCAACGTG
    CTCTTCGTCA ACTCTGTGGA CATGGAGTCA CTCACTGGGC CACAGGCCAT CTCCAAAGCC
    ATTTCTGAGA CCCTGGCTGC TGACCCCACA CCAGCTGCCA CCATCGTTCA CTTCAAAGTT
    TCTGCCCAGG GAATTACACT GACTGACAAC CAGAGAAAGC TTTTCTTCAG GCGGCACTAC
    CCCCTCAACA CTGTGACCTT CTGTGACCTG GATCCACAGG AGAGGAAGTG GACAAAGACG
    GAGGGAGGTG CCCCTGCTAA GCTCTTCGGT TTTGTGGCCC GGAAACAGGG CAGCGCCACG
    GACAACGCCT GCCACCTCTT TGCTGAGCTT GACCCCAACC AGCCGGCCTC CGCCATCGTC
    AGCTTTGTCT CCAAGGTCAT GCTGAGTGCC GGCCAGAAGA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa41509
    Clone ID Related Accession (Same CDS sequence) NM_174766.2
    Accession Version NM_174766.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-15
    Organism Bos taurus(cattle)
    Product tensin-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF225897.1. On Jun 3, 2003 this sequence version replaced NM_174766.1. ##Evidence-Data-START## Transcript exon combination :: AF225897.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCACGCG
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGCA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCCAGGGACC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCAATGGG
    GCTGTCAATG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GGCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGACTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGGGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGGCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAGGGGTGGG GTCCTTAGCG TCGAGAGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GGGGTGCAGG CTCGGGAGAA GCAGCCTGCA GAGCCCCCAG CACCTCTCCG GAAGCGGGCT
    GCCAGTGATG GGCAGTATGA GAACCAGTCA CCAGAACCCA CATCCCCCCG TAGCCCTGGG
    GTTCGCTCGC CTGTCCAATG TGTCTCCCCA GAGCTGGCTC TTACCATCGC TCTCAATCCT
    GGAGGGCGAC CCAAAGAGCC CCATCTGCAC AGCTACAAGG AAGCCTTTGA GGAGATGGAG
    GGAACCTCTC CGACCAGCCC ACCACCCAGT GGGGTGCGTT CCCCTCCAGG TCTGGCCAAG
    ACACCCTTGT CTGCTCTGGG CCTGAAGCCC CACAACCCAG CCGACATCCT GCTGCATCCC
    ACTGGTGAGC CCCGGAGCTA CGTGGAGTCG GTGGTACGAA CGGCGGTGGC TGGGCCCCGG
    ACTCAGGAAC CTGAGCCCAA GAGCTTTAGC GCCCCAGCTG CGCAGGCGTA TGGCCATGAG
    ACACCCCTGA GGATTGGGAC CCTGGGAGGC TCCTTTGTCT CCCCTAGCCC CCTCTCCACC
    AGCAGCCCCA TCCTCAGTGC TGACAGCACT TCAGTGGGGA GTTTCCCATC CGGGGAGAGC
    AGCGACCAGG GCGCGCGAAC ACCTACCCAG CCTCTGCTGG ATTCTGGCTT CCGCTCCGGC
    AGCCTGGGCC AGCCAAGTCC TTTGGCTCAG AGAAACTACC AGAGCTCTTC TCCTCTCCCA
    ACTGCAGGCA GCAGCTACAG CAGCCCCGAC TACTCACTGC AGCAGTTCAG CTCCCCAGAA
    GGCCAGGCCC GGTCTCAGTT CAGTGTGGCC GGTGTCCACA CAGTGCCTGG GAGCCCCCAG
    GCACGGCACA GGACAGTGGG CACCAATACA CCCCCGAGCC CTGGCTTTGG CCGTCGGGCT
    GTGAACCCCA GCCTGGCTGC CCCCAGCAGC CCCAGTTTGA GCCACCGCCA GGTGATGGGT
    CCACTGGGAA CCGGTTTCCA TGGTAACACG GGCTCCAGCC CCCAGAGCAG TGCAGCAACC
    ACTCCGGGAA GCCCCAGTCT GGGTCGCCAC CCTGGGGCCC AGGTTTCCAA CCTCCATGGC
    AATGTGGTCA CCCGTCCTGG GAGCCCCAGC CTGGGCCGGC ACCCCGGGGC CCACCAAGGC
    ACCCTGGCCT CCAATCTCCA TAGCAATGCA GTAGCCAGCC CCGGAAGCCC CAGCCTGGGC
    CGTCACTTGG GAGGGTCTGG ATCTGTGGTT CCCGGCAGCC CCAGCTTGGA CCGGCACGTG
    CCCTATGGTG GCTACTCCAC CCCTGAGGAC CGGAGACCCA CGCTGTCCCG GCAGAGCAGC
    GCCTCAGGCT ACCAGGCTCC TTCCACGCCC TCCTTCCCTG TGTCCCCCGC CTACTACCCT
    GGCCTGAGCA GCCCCGCCAC CTCCCCGTCG CCTGATTCGG CAGCTTTCCG ACAAGGGAGC
    CCCACCCCAG CCTTGCCGGA GAAGCGGAGG ATGTCCATGG GCGACCGAGC GGGCAGCCTC
    CCCAACTACG CCACCGTCAA CGGGAAGGTG TCCTCCTCGC CGGTCGCCAG TGGCATGTCC
    AGTCCCAGCG GGGGCAGCAC CGTCTCCTTC TCCCACACTC TGCCCGACTT CTCCAAGTAC
    TCCATGCCAG ACAACAGTCC TGAGACGCGG GCTAAAGTGA AGTTTGTCCA AGACACTTCC
    AAGTATTGGT ACAAGCCTGA GATCTCCAGA GAACAGGCCA TCGCCCTCCT TAAGGACCAG
    GAGCCAGGGG CTTTCATCAT CCGTGACAGC CACTCCTTCC GAGGAGCCTA TGGGCTGGCC
    ATGAAGGTGT CTTCGCCCCC TCCGACCATC ATGCAGCAGA ATAAAAAAGG GGACATGACC
    CACGAGCTCG TGAGGCATTT CCTGATAGAG ACCGGCCCCA GAGGAGTCAA GCTCAAGGGC
    TGCCCCAATG AGCCAAACTT CGGATCTCTG TCTGCCCTGG TCTACCAGCA CTCCATCATC
    CCGCTGGCCC TGCCCTGCAA GCTGGTCATT CCAAACCGAG ACCCCACAGA TGAATCGAAA
    GATAGCTCGG GCCCTGCCAA CTCAACCAGT GACCTGCTGA AGCAAGGGGC AGCCTGCAAC
    GTGCTCTTCG TCAACTCTGT GGACATGGAG TCACTCACAG GTCCACAGGC CATCTCCAAA
    GCCATTTCTG AGACCCTGGC TGCTGACCCC ACACCAGCTG CCACCATCGT TCACTTCAAA
    GTTTCTGCCC AGGGAATTAC ACTGACTGAC AACCAGAGAA AGCTTTTCTT CAGGCGGCAC
    TACCCCCTCA ACACTGTGAC CTTCTGTGAC CTGGATCCAC AGGAGAGGAA GTGGACAAAG
    ACGGAGGGAG GTGCCCCTGC TAAGCTCTTC GGTTTTGTGG CCCGGAAACA GGGCAGCGCC
    ACGGACAACG CCTGCCACCT CTTTGCTGAG CTTGACCCCA ACCAGCCGGC CTCCGCCATC
    GTCAGCTTTG TCTCCAAGGT CATGCTGAGT GCCGGCCAGA AGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_777191.1
    CDS365..5512
    Translation

    Target ORF information:

    RefSeq Version NM_174766.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tensin 1 (TNS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_174766.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAGTACGA GCCGGACCAC GGAGGACAGC TGTGAGCTGG ACCTGGTGTA TGTCACCGAG 
    AGGATCATCG CCGTCTCCTT CCCGAGCACA GCCAATGAGG AGAACTTCCG GAGCAACCTC
    CGAGAGGTGG CCCAGATGCT CAAGTCCAAA CATGGAGGCA ACTACCTGCT TTTCAACCTC
    TCTGAGCGGA GACCTGACAT CACGAAGCTC CACGCCAAGG TCCTGGAATT TGGCTGGCCC
    GACCTCCACA CCCCGGCCCT GGAGAAGATC TGCAGCGTCT GCAAGGCCAT GGACACCTGG
    CTCAATGCAG ACCCCCACAA CGTGGTCGTG CTACACAACA AGGGAAACCG AGGCAGGATC
    GGCGTTGTGA TCGCGGCTTA CATGCACTAC AGCAACATTT CTGCCAGTGC CGACCACGCG
    CTGGACCGGT TTGCGATGAA ACGGTTCTAC GAGGATAAGA TTGTACCCAT TGGCCAACCA
    TCCCAGAGGA GGTACGTGCA TTATTTCAGC GGCCTGCTCT CTGGCTCCAT CAAAATGAAC
    AACAAGCCCT TGTTTCTGCA CCACGTGATC ATGCACGGCA TCCCCAACTT CGAGTCTAAA
    GGAGGGTGTC GACCATTTCT CCGGATCTAC CAGGCCATGC AACCTGTCTA CACATCTGGC
    ATCTACAACG TCCAAGGAGA CAGCCAGACC AGCATCTGCA TCACCATTGA GCCTGGGCTG
    CTCTTGAAGG GAGACATCTT GCTGAAGTGC TACCACAAGA AGTTCCGGAG CCCAGCCCGA
    GATGTCATCT TCAGGGTGCA GTTCCACACC TGTGCCATCC ATGACCTGGG AGTTGTCTTT
    GGGAAGGAGG ACCTTGACGA TGCCTTCAAG GACGATCGAT TTCCAGAATA CGGCAAAGTT
    GAATTTGTAT TTTCTTATGG ACCAGAGAAA ATTCAAGGCA TGGAACACCT GGAGAATGGC
    CCGAGCGTGT CCGTGGACTA TAACACCTCT GACCCCCTCA TCCGCTGGCA TTCCTATGAC
    AACTTCAATG GGCATCGAGA TGATGGCATG GAGGAGGTTG TGGGCCATAC CCCAGGGACC
    CTGGACGGGA GCCTGTATGC CAAGGTGAAG AAGAAGGACT CCTTGCACGG CAGCAATGGG
    GCTGTCAATG CCACGCGGCC CGTGCTGTCA GCCACCCCCA ATCACGTGGA GCACACCCTC
    TCCGTGAGCA GTGACTCGGG CAACTCCACA GCCTCCACCA AGACAGACAA GACCGATGAG
    CCTGCCCCCG GGCCTGCCAG TGCCCCTGCT GCCCTGAGTC CCGAGGAGAA GCGTGAGCTG
    GACCGACTGC TCAGTGGCTT TGGCTTGGAG CGGGAGAAGC AAGGGGCCAT GTACCACCCT
    CAGCATCTCC GGTCCCGGCC AGTGGGAGGC CCTGCTGCCC CTTCCTCTGG CCGCCACATC
    GTCCCGGCCC AGGTTCACGT CAGGGGTGGG GTCCTTAGCG TCGAGAGGGA GACAGACATC
    CTGGATGATG AGCTGCCCAA CCAGGATGGG CATAGCGTGG GCAGCATGGG CACCCTGTCC
    TCCCTGGATG GAGTCACCAA CACAAGTGAG GGTGGCTACC CGGAGGCCCT CTCCCCGCTG
    ACCAACGGTC TGGACAAGCC CTACCCCATG GAACCTATGG TCAACGGTGG GGGCTACCCC
    TATGAGTCTG CCAGCCGGGC AGTCTCTGCC CAGGCTGGCC ACACAGCCCC CATGAGACCC
    TCCTACTCCA CACAGGAGGG TTTAGCTGGC TACCAGCGGG AGGGACCCCA CCCAGCCTGG
    CCACAGTCAG CGACCACCTC CCACTATGGC CATGACCCCA ACGGTATGTT TCGCTCTCAA
    TCCTTTCCTG AAACGGAGCC CCAGCTGCCC CCAGCTCCAG CCCGTGGTGG GAGCAGCCGG
    GAGGCTGTGC AGAGGGGTCT GAATTCCTGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAGCAGCCTC GCCCACCTCC TCGCCAACAG GAAAGAGTCC ACTTGGAGAG CCTTGGGCTC
    AGCAGGCCCA GCCCCCAGCC ACTAGCAGAG CCCCCCATGT CTGGCCTCCC TGAGTTCCCT
    CGCGCAGCCT CCCAGCAGGA GATCGAGCAG TCCATCGAAG CCCTCAATAT GCTGATGCTC
    GATTTGGAAC CAGCCACGGC GGGCGCCCCC CTACACAAGT CCCAGAGCGT GCCTGGGGCC
    TGGCCAGGGG CTTCTCCGCT CTCTTCCCAG CCCTTCTCTG GCTCCTCTTG CCAGTCCCAC
    CCACTGACCC AGTCCAGATC TGGCTACATC CCCAGTGGGC ATTCCTTGGG AACCCCTGAG
    CCAGCCCCCC GGGCCCCCCT GGAGTCTGTT CCCACTGGCA GGCCTTACTC ACCTTATGAT
    TATCAGCCAT GTCCAACTGG GCCCAACCAG AGTTACCATC CAAAGAGCCC GGCCACCTCC
    TCCTCGTCTT CCTTCCTTCC AACCACCCAG AGCTCTGTGG GGCCTCAGCA GCCCCCAGCC
    TCTCTCCCTG GCCTCACCAC TCAGCCTCAG CTTCCACCAA AGGAGGTGAC TTCAGACCCA
    TCCCGAACTC CAGAGGAGGA ACCATTGAAC TTGGAAGGGC TGGTGGCCCA CCGGGTGGCA
    GGGGTGCAGG CTCGGGAGAA GCAGCCTGCA GAGCCCCCAG CACCTCTCCG GAAGCGGGCT
    GCCAGTGATG GGCAGTATGA GAACCAGTCA CCAGAACCCA CATCCCCCCG TAGCCCTGGG
    GTTCGCTCGC CTGTCCAATG TGTCTCCCCA GAGCTGGCTC TTACCATCGC TCTCAATCCT
    GGAGGGCGAC CCAAAGAGCC CCATCTGCAC AGCTACAAGG AAGCCTTTGA GGAGATGGAG
    GGAACCTCTC CGACCAGCCC ACCACCCAGT GGGGTGCGTT CCCCTCCAGG TCTGGCCAAG
    ACACCCTTGT CTGCTCTGGG CCTGAAGCCC CACAACCCAG CCGACATCCT GCTGCATCCC
    ACTGGTGAGC CCCGGAGCTA CGTGGAGTCG GTGGTACGAA CGGCGGTGGC TGGGCCCCGG
    ACTCAGGAAC CTGAGCCCAA GAGCTTTAGC GCCCCAGCTG CGCAGGCGTA TGGCCATGAG
    ACACCCCTGA GGATTGGGAC CCTGGGAGGC TCCTTTGTCT CCCCTAGCCC CCTCTCCACC
    AGCAGCCCCA TCCTCAGTGC TGACAGCACT TCAGTGGGGA GTTTCCCATC CGGGGAGAGC
    AGCGACCAGG GCGCGCGAAC ACCTACCCAG CCTCTGCTGG ATTCTGGCTT CCGCTCCGGC
    AGCCTGGGCC AGCCAAGTCC TTTGGCTCAG AGAAACTACC AGAGCTCTTC TCCTCTCCCA
    ACTGCAGGCA GCAGCTACAG CAGCCCCGAC TACTCACTGC AGCAGTTCAG CTCCCCAGAA
    GGCCAGGCCC GGTCTCAGTT CAGTGTGGCC GGTGTCCACA CAGTGCCTGG GAGCCCCCAG
    GCACGGCACA GGACAGTGGG CACCAATACA CCCCCGAGCC CTGGCTTTGG CCGTCGGGCT
    GTGAACCCCA GCCTGGCTGC CCCCAGCAGC CCCAGTTTGA GCCACCGCCA GGTGATGGGT
    CCACTGGGAA CCGGTTTCCA TGGTAACACG GGCTCCAGCC CCCAGAGCAG TGCAGCAACC
    ACTCCGGGAA GCCCCAGTCT GGGTCGCCAC CCTGGGGCCC AGGTTTCCAA CCTCCATGGC
    AATGTGGTCA CCCGTCCTGG GAGCCCCAGC CTGGGCCGGC ACCCCGGGGC CCACCAAGGC
    ACCCTGGCCT CCAATCTCCA TAGCAATGCA GTAGCCAGCC CCGGAAGCCC CAGCCTGGGC
    CGTCACTTGG GAGGGTCTGG ATCTGTGGTT CCCGGCAGCC CCAGCTTGGA CCGGCACGTG
    CCCTATGGTG GCTACTCCAC CCCTGAGGAC CGGAGACCCA CGCTGTCCCG GCAGAGCAGC
    GCCTCAGGCT ACCAGGCTCC TTCCACGCCC TCCTTCCCTG TGTCCCCCGC CTACTACCCT
    GGCCTGAGCA GCCCCGCCAC CTCCCCGTCG CCTGATTCGG CAGCTTTCCG ACAAGGGAGC
    CCCACCCCAG CCTTGCCGGA GAAGCGGAGG ATGTCCATGG GCGACCGAGC GGGCAGCCTC
    CCCAACTACG CCACCGTCAA CGGGAAGGTG TCCTCCTCGC CGGTCGCCAG TGGCATGTCC
    AGTCCCAGCG GGGGCAGCAC CGTCTCCTTC TCCCACACTC TGCCCGACTT CTCCAAGTAC
    TCCATGCCAG ACAACAGTCC TGAGACGCGG GCTAAAGTGA AGTTTGTCCA AGACACTTCC
    AAGTATTGGT ACAAGCCTGA GATCTCCAGA GAACAGGCCA TCGCCCTCCT TAAGGACCAG
    GAGCCAGGGG CTTTCATCAT CCGTGACAGC CACTCCTTCC GAGGAGCCTA TGGGCTGGCC
    ATGAAGGTGT CTTCGCCCCC TCCGACCATC ATGCAGCAGA ATAAAAAAGG GGACATGACC
    CACGAGCTCG TGAGGCATTT CCTGATAGAG ACCGGCCCCA GAGGAGTCAA GCTCAAGGGC
    TGCCCCAATG AGCCAAACTT CGGATCTCTG TCTGCCCTGG TCTACCAGCA CTCCATCATC
    CCGCTGGCCC TGCCCTGCAA GCTGGTCATT CCAAACCGAG ACCCCACAGA TGAATCGAAA
    GATAGCTCGG GCCCTGCCAA CTCAACCAGT GACCTGCTGA AGCAAGGGGC AGCCTGCAAC
    GTGCTCTTCG TCAACTCTGT GGACATGGAG TCACTCACAG GTCCACAGGC CATCTCCAAA
    GCCATTTCTG AGACCCTGGC TGCTGACCCC ACACCAGCTG CCACCATCGT TCACTTCAAA
    GTTTCTGCCC AGGGAATTAC ACTGACTGAC AACCAGAGAA AGCTTTTCTT CAGGCGGCAC
    TACCCCCTCA ACACTGTGAC CTTCTGTGAC CTGGATCCAC AGGAGAGGAA GTGGACAAAG
    ACGGAGGGAG GTGCCCCTGC TAAGCTCTTC GGTTTTGTGG CCCGGAAACA GGGCAGCGCC
    ACGGACAACG CCTGCCACCT CTTTGCTGAG CTTGACCCCA ACCAGCCGGC CTCCGCCATC
    GTCAGCTTTG TCTCCAAGGT CATGCTGAGT GCCGGCCAGA AGAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.