tns1b gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021479027.1	XP_021334702.1	tensin-1 isoform X1
XM_021479033.1	XP_021334708.1	tensin-1 isoform X7
XM_021479035.1	XP_021334710.1	tensin-1 isoform X9
XM_017357846.1	XP_017213335.1	tensin-1 isoform X1
XM_017357848.1	XP_017213337.1	tensin-1 isoform X3
XM_017357849.1	XP_017213338.1	tensin-1 isoform X4
XM_017357850.1	XP_017213339.1	tensin-1 isoform X7
XM_009304966.2	XP_009303241.1	tensin-1 isoform X8
XM_021479030.1	XP_021334705.1	tensin-1 isoform X4
XM_021479036.1	XP_021334711.1	tensin-1 isoform X10
XM_005167931.3	XP_005167988.2	tensin-1 isoform X6
XM_021479028.1	XP_021334703.1	tensin-1 isoform X2
XM_021479029.1	XP_021334704.1	tensin-1 isoform X3
XM_021479031.1	XP_021334706.1	tensin-1 isoform X5
XM_021479037.1	XP_021334712.1	tensin-1 isoform X11
XM_017357847.1	XP_017213336.1	tensin-1 isoform X2
XM_009304969.2	XP_009303244.2	tensin-1 isoform X5
XM_021479032.1	XP_021334707.1	tensin-1 isoform X6
XM_021479034.1	XP_021334709.1	tensin-1 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa144113	XM_021479027.1 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144114	XM_021479028.1 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144115	XM_021479029.1 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144116	XM_021479030.1 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144117	XM_021479031.1 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144118	XM_021479032.1 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144119	XM_021479033.1 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144120	XM_021479034.1 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144121	XM_021479035.1 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144122	XM_021479036.1 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144123	XM_021479037.1 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa60375	XM_017357846.1 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa60376	XM_017357847.1 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa60377	XM_017357848.1 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa60378	XM_017357849.1 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa60379	XM_009304969.2 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa60380	XM_005167931.3 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa60381	XM_017357850.1 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa42135	XM_009304966.2 Latest version!	Danio rerio tensin 1b (tns1b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

tns1b ( XM_021479027.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334702.1 Danio rerio tensin 1b (tns1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa144113
Clone ID Related Accession (Same CDS sequence)	XM_021479027.1
Accession Version	XM_021479027.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA 
GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT 
TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT 
CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC 
CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG 
AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG 
AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT 
AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC 
AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG 
CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG 
ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG 
GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT 
TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT 
TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC 
AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC 
ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC 
TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG 
ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG 
TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC 
ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT 
AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA 
AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC 
CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC 
ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT 
AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC 
ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT 
GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG 
CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG 
GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT 
GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC 
AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC 
CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT 
CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA 
GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA 
GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG 
TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA 
ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA 
GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG 
TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG 
ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA 
GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC 
CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC 
TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT 
ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC 
ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA 
AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA 
TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA 
GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT 
CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG 
TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA 
GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG 
CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC 
TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT 
GGTGAGGTCC TACCCCAAAC CCCTGCCTTC CCTGTCTCGC CACAAACCCC TTACTTCAAC 
ATGTGTCGAT CTCCTCCAGG ATTGGCTAAA ACACCACTGT CCTTTCTGGG CCTGAAGCCC 
CACAACCCTG CTGAGATCCT GCTCAACCAG ACAGGCTCAG AGAGTGAGGA TGAGGAAGAG 
CCTCGTAGTT ATGTGGAGTC TGTGGCGAAG GCAGCAGCAG TACCTGCATC TCCAACATAT 
GACAGCGACA GCTTAGCTCG TCCTGCCGGA CCGTCCCATG CTTACAATGC TCCACTTTCC 
TCCAGCAGCC CCATTCAGAA CCCAGATCCA GTTGAAAGCA CATTGGCTGA CAGCGGGGTC 
GGGATGAATT CTTTATCCCA GGCTGCTGTC GACATTTTCC ATTTCCAGCC CTCCGAAATC 
CCATACCAAC CACCACCACC CCCCTACCCA ACCTCCATGT TGGGTAGCTA CATCAGCCCT 
TCTCCAGCAG AGGCCATGCA AAATAATACC ACCACCATCC AGCCACTTTC TGGAAGTCCT 
GGGACAAGAC ACCACACCAT CCTTTCTGAC ATGGCGTCCG TTCCCAACTT TCAACACAGA 
TTAGCAAATC AGGATGGTCC CATACTTGGG CGACAGCAGG CTCCAGCTAA TGGTCATGTG 
CCTGGAATTG CTGGTAGTCC AGTGCAAGGA AGACACACTA TGGTGTCCCA GGGTACACAA 
AGCAGTCCCA TACTGAGCAG ACAGTATCCT GTCACCCAGG GGATTCAGAG TAGTCCAATT 
CTCAGCAGGC AGCCAATGGG ACAAGCTGTC CAGAGCAGTC CAGTCCTCAA CCGACAGGCA 
TCCCTGAATC AAGCAAACCA AAGCAGTCCA GTGTTGAGTA GACAGCAGTC CATTACCCTA 
CCCAACCAAG GCAGCCCGGT CCTAAGCAGA CAGTCCTCGC TTAGTCATCC CAGTCAGGGT 
AGTCCTGTAC TAGGACGCCA CCCTTCCCTA ACACAGGGTA GCCCCAGCCT GGATAGGCAT 
CCGATGTACA GCGGTTACAC CACTCCAGAA GAGCGACACG GAGCTCTGTC ACGCCAAAGC 
AGCTCTTCAG GATACCAGCC GCCATCTACA CCGTCTTTCC CAGTTTCTCC AGCAGGATAC 
ATCGAGGGAG CGGGATTCAG ACAGGGGAGT CCTGCTTTCC AACCTCAGTT GCCCGAAAAG 
AGACGCATGT CCAGCAGTGA CCGCACCAAT GGTGGCCTGT CCTATGGGAC GCTCAATGGG 
AAGATGTCCT CACCGGTGTC CAGTGGCGGC AGCACTCCGA GTGTTCATTT CTTCCACACA 
CTCCCCGATT TCTCCAAACT CAACATGTGT GATGGAAGTC CAGAAACCAG AATGAACGTG 
AAGTTTGTCC AGGACACGTC CAAATTCTGG TATAAACCAG ATATCTCGAG AGACCAAGCG 
ATTAACATGC TGAAGGATCA AGATCCTGGG GCCTTTGTGA TTCGGGACAG TCATTCATTC 
AGAGGAGCCT ACGGCTTGGC TATGAAAGTG GCTTCACCAC CTCCAACCAC TCAACCTACA 
AAGAAAGCTG GAGATGTCAC TAATGAGTTG GTGAGGCATT TTCTAATTGA GACGAGTCCG 
AAGGGTGTGA GACTGAAGGG CTGCCCCAAC GAGCCGTACT TTGGCTGCCT GTCTGCGCTG 
GTCTACCAGC ACTCCATCAC TCCACTGGCA CTGCCCTGCA AACTAGTCAT ACCAACCAGA 
GATCCTCTGG AAGAGTCACC TGAGATCGCA ACACCAACCA ACCCGGCGGC AGATATGCTC 
AAGCAGGGCG CAGGGCAGAA GAGTTCAGCT GATGGCCATG CATGCAATGT GCTGTACATC 
AACTCTGTGG ATATGGAGTC TTTGACTGGA CCTCAAGCCA TCGCCAAAGC CATTTCAGAA 
ACCATGAACG CCAGTCCGGC ACCCTCAGCC ACCATTGTTC ACTTCAAGGT GTCGGCACAA 
GGCATCACAC TCACAGACAA CCAGAGAAAG CTTTTCTTCA GACGTCATTA TCCCATCGGC 
ACAGTCACGT TCTGTGACAT TGATCCACAG GAGAGGAAGT GGAACAAACC AGAGGGTGGC 
ACTGCCAAAT TCTTTGGCTT TGTGGCTAGA AAGCAAGGCA GCACGACAGA CAATGTCAGT 
CACCTGTTTG CTGAGATGGA CCCAGATCAG CCGGCCACCG CCATCGTCAA CTTTGTCTCT 
AAAGTGATGA TCAGCTCTCA GAAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334702.1
CDS	285..5774
Translation	MGCTVSFICCEDDFLQMPLDQHVEKKKEEKRLLFKPEEFEAVHSHTFRLKTFKKGKHCGVCKQTVNNEGLICRVCRLACHRKCEVKVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAEYTARIRCINDSQSAPEFGHRRRSLSFSGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGEVLPQTPAFPVSPQTPYFNMCRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSESEDEEEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAGQKSSADGHACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_021479027.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479027.1

ORF Insert Sequence:

ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA 
GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT 
TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT 
CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC 
CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG 
AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG 
AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT 
AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC 
AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG 
CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG 
ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG 
GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT 
TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT 
TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC 
AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC 
ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC 
TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG 
ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG 
TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC 
ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT 
AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA 
AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC 
CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC 
ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT 
AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC 
ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT 
GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG 
CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG 
GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT 
GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC 
AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC 
CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT 
CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA 
GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA 
GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG 
TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA 
ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA 
GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG 
TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG 
ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA 
GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC 
CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC 
TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT 
ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC 
ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA 
AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA 
TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA 
GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT 
CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG 
TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA 
GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG 
CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC 
TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT 
GGTGAGGTCC TACCCCAAAC CCCTGCCTTC CCTGTCTCGC CACAAACCCC TTACTTCAAC 
ATGTGTCGAT CTCCTCCAGG ATTGGCTAAA ACACCACTGT CCTTTCTGGG CCTGAAGCCC 
CACAACCCTG CTGAGATCCT GCTCAACCAG ACAGGCTCAG AGAGTGAGGA TGAGGAAGAG 
CCTCGTAGTT ATGTGGAGTC TGTGGCGAAG GCAGCAGCAG TACCTGCATC TCCAACATAT 
GACAGCGACA GCTTAGCTCG TCCTGCCGGA CCGTCCCATG CTTACAATGC TCCACTTTCC 
TCCAGCAGCC CCATTCAGAA CCCAGATCCA GTTGAAAGCA CATTGGCTGA CAGCGGGGTC 
GGGATGAATT CTTTATCCCA GGCTGCTGTC GACATTTTCC ATTTCCAGCC CTCCGAAATC 
CCATACCAAC CACCACCACC CCCCTACCCA ACCTCCATGT TGGGTAGCTA CATCAGCCCT 
TCTCCAGCAG AGGCCATGCA AAATAATACC ACCACCATCC AGCCACTTTC TGGAAGTCCT 
GGGACAAGAC ACCACACCAT CCTTTCTGAC ATGGCGTCCG TTCCCAACTT TCAACACAGA 
TTAGCAAATC AGGATGGTCC CATACTTGGG CGACAGCAGG CTCCAGCTAA TGGTCATGTG 
CCTGGAATTG CTGGTAGTCC AGTGCAAGGA AGACACACTA TGGTGTCCCA GGGTACACAA 
AGCAGTCCCA TACTGAGCAG ACAGTATCCT GTCACCCAGG GGATTCAGAG TAGTCCAATT 
CTCAGCAGGC AGCCAATGGG ACAAGCTGTC CAGAGCAGTC CAGTCCTCAA CCGACAGGCA 
TCCCTGAATC AAGCAAACCA AAGCAGTCCA GTGTTGAGTA GACAGCAGTC CATTACCCTA 
CCCAACCAAG GCAGCCCGGT CCTAAGCAGA CAGTCCTCGC TTAGTCATCC CAGTCAGGGT 
AGTCCTGTAC TAGGACGCCA CCCTTCCCTA ACACAGGGTA GCCCCAGCCT GGATAGGCAT 
CCGATGTACA GCGGTTACAC CACTCCAGAA GAGCGACACG GAGCTCTGTC ACGCCAAAGC 
AGCTCTTCAG GATACCAGCC GCCATCTACA CCGTCTTTCC CAGTTTCTCC AGCAGGATAC 
ATCGAGGGAG CGGGATTCAG ACAGGGGAGT CCTGCTTTCC AACCTCAGTT GCCCGAAAAG 
AGACGCATGT CCAGCAGTGA CCGCACCAAT GGTGGCCTGT CCTATGGGAC GCTCAATGGG 
AAGATGTCCT CACCGGTGTC CAGTGGCGGC AGCACTCCGA GTGTTCATTT CTTCCACACA 
CTCCCCGATT TCTCCAAACT CAACATGTGT GATGGAAGTC CAGAAACCAG AATGAACGTG 
AAGTTTGTCC AGGACACGTC CAAATTCTGG TATAAACCAG ATATCTCGAG AGACCAAGCG 
ATTAACATGC TGAAGGATCA AGATCCTGGG GCCTTTGTGA TTCGGGACAG TCATTCATTC 
AGAGGAGCCT ACGGCTTGGC TATGAAAGTG GCTTCACCAC CTCCAACCAC TCAACCTACA 
AAGAAAGCTG GAGATGTCAC TAATGAGTTG GTGAGGCATT TTCTAATTGA GACGAGTCCG 
AAGGGTGTGA GACTGAAGGG CTGCCCCAAC GAGCCGTACT TTGGCTGCCT GTCTGCGCTG 
GTCTACCAGC ACTCCATCAC TCCACTGGCA CTGCCCTGCA AACTAGTCAT ACCAACCAGA 
GATCCTCTGG AAGAGTCACC TGAGATCGCA ACACCAACCA ACCCGGCGGC AGATATGCTC 
AAGCAGGGCG CAGGGCAGAA GAGTTCAGCT GATGGCCATG CATGCAATGT GCTGTACATC 
AACTCTGTGG ATATGGAGTC TTTGACTGGA CCTCAAGCCA TCGCCAAAGC CATTTCAGAA 
ACCATGAACG CCAGTCCGGC ACCCTCAGCC ACCATTGTTC ACTTCAAGGT GTCGGCACAA 
GGCATCACAC TCACAGACAA CCAGAGAAAG CTTTTCTTCA GACGTCATTA TCCCATCGGC 
ACAGTCACGT TCTGTGACAT TGATCCACAG GAGAGGAAGT GGAACAAACC AGAGGGTGGC 
ACTGCCAAAT TCTTTGGCTT TGTGGCTAGA AAGCAAGGCA GCACGACAGA CAATGTCAGT 
CACCTGTTTG CTGAGATGGA CCCAGATCAG CCGGCCACCG CCATCGTCAA CTTTGTCTCT 
AAAGTGATGA TCAGCTCTCA GAAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_021479028.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334703.1 Danio rerio tensin 1b (tns1b), transcript variant X2, mRNA.

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CloneID	ODa144114
Clone ID Related Accession (Same CDS sequence)	XM_021479028.1
Accession Version	XM_021479028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA 
GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT 
TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT 
CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC 
CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG 
AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG 
AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT 
AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC 
AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG 
CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG 
ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG 
GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT 
TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT 
TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC 
AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC 
ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC 
TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG 
ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG 
TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC 
ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT 
AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA 
AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC 
CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC 
ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT 
AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC 
ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT 
GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG 
CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG 
GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT 
GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC 
AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC 
CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT 
CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA 
GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA 
GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG 
TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA 
ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA 
GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG 
TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG 
ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA 
GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC 
CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC 
TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT 
ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC 
ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA 
AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA 
TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA 
GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT 
CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG 
TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA 
GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG 
CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC 
TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT 
GGTGAGGTCC TACCCCAAAC CCCTGCCTTC CCTGTCTCGC CACAAACCCC TTACTTCAAC 
ATGTGTCGAT CTCCTCCAGG ATTGGCTAAA ACACCACTGT CCTTTCTGGG CCTGAAGCCC 
CACAACCCTG CTGAGATCCT GCTCAACCAG ACAGGCTCAG AGCCTCGTAG TTATGTGGAG 
TCTGTGGCGA AGGCAGCAGC AGTACCTGCA TCTCCAACAT ATGACAGCGA CAGCTTAGCT 
CGTCCTGCCG GACCGTCCCA TGCTTACAAT GCTCCACTTT CCTCCAGCAG CCCCATTCAG 
AACCCAGATC CAGTTGAAAG CACATTGGCT GACAGCGGGG TCGGGATGAA TTCTTTATCC 
CAGGCTGCTG TCGACATTTT CCATTTCCAG CCCTCCGAAA TCCCATACCA ACCACCACCA 
CCCCCCTACC CAACCTCCAT GTTGGGTAGC TACATCAGCC CTTCTCCAGC AGAGGCCATG 
CAAAATAATA CCACCACCAT CCAGCCACTT TCTGGAAGTC CTGGGACAAG ACACCACACC 
ATCCTTTCTG ACATGGCGTC CGTTCCCAAC TTTCAACACA GATTAGCAAA TCAGGATGGT 
CCCATACTTG GGCGACAGCA GGCTCCAGCT AATGGTCATG TGCCTGGAAT TGCTGGTAGT 
CCAGTGCAAG GAAGACACAC TATGGTGTCC CAGGGTACAC AAAGCAGTCC CATACTGAGC 
AGACAGTATC CTGTCACCCA GGGGATTCAG AGTAGTCCAA TTCTCAGCAG GCAGCCAATG 
GGACAAGCTG TCCAGAGCAG TCCAGTCCTC AACCGACAGG CATCCCTGAA TCAAGCAAAC 
CAAAGCAGTC CAGTGTTGAG TAGACAGCAG TCCATTACCC TACCCAACCA AGGCAGCCCG 
GTCCTAAGCA GACAGTCCTC GCTTAGTCAT CCCAGTCAGG GTAGTCCTGT ACTAGGACGC 
CACCCTTCCC TAACACAGGG TAGCCCCAGC CTGGATAGGC ATCCGATGTA CAGCGGTTAC 
ACCACTCCAG AAGAGCGACA CGGAGCTCTG TCACGCCAAA GCAGCTCTTC AGGATACCAG 
CCGCCATCTA CACCGTCTTT CCCAGTTTCT CCAGCAGGAT ACATCGAGGG AGCGGGATTC 
AGACAGGGGA GTCCTGCTTT CCAACCTCAG TTGCCCGAAA AGAGACGCAT GTCCAGCAGT 
GACCGCACCA ATGGTGGCCT GTCCTATGGG ACGCTCAATG GGAAGATGTC CTCACCGGTG 
TCCAGTGGCG GCAGCACTCC GAGTGTTCAT TTCTTCCACA CACTCCCCGA TTTCTCCAAA 
CTCAACATGT GTGATGGAAG TCCAGAAACC AGAATGAACG TGAAGTTTGT CCAGGACACG 
TCCAAATTCT GGTATAAACC AGATATCTCG AGAGACCAAG CGATTAACAT GCTGAAGGAT 
CAAGATCCTG GGGCCTTTGT GATTCGGGAC AGTCATTCAT TCAGAGGAGC CTACGGCTTG 
GCTATGAAAG TGGCTTCACC ACCTCCAACC ACTCAACCTA CAAAGAAAGC TGGAGATGTC 
ACTAATGAGT TGGTGAGGCA TTTTCTAATT GAGACGAGTC CGAAGGGTGT GAGACTGAAG 
GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC CTGTCTGCGC TGGTCTACCA GCACTCCATC 
ACTCCACTGG CACTGCCCTG CAAACTAGTC ATACCAACCA GAGATCCTCT GGAAGAGTCA 
CCTGAGATCG CAACACCAAC CAACCCGGCG GCAGATATGC TCAAGCAGGG CGCAGGGCAG 
AAGAGTTCAG CTGATGGCCA TGCATGCAAT GTGCTGTACA TCAACTCTGT GGATATGGAG 
TCTTTGACTG GACCTCAAGC CATCGCCAAA GCCATTTCAG AAACCATGAA CGCCAGTCCG 
GCACCCTCAG CCACCATTGT TCACTTCAAG GTGTCGGCAC AAGGCATCAC ACTCACAGAC 
AACCAGAGAA AGCTTTTCTT CAGACGTCAT TATCCCATCG GCACAGTCAC GTTCTGTGAC 
ATTGATCCAC AGGAGAGGAA GTGGAACAAA CCAGAGGGTG GCACTGCCAA ATTCTTTGGC 
TTTGTGGCTA GAAAGCAAGG CAGCACGACA GACAATGTCA GTCACCTGTT TGCTGAGATG 
GACCCAGATC AGCCGGCCAC CGCCATCGTC AACTTTGTCT CTAAAGTGAT GATCAGCTCT 
CAGAAGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334703.1
CDS	284..5755
Translation	MGCTVSFICCEDDFLQMPLDQHVEKKKEEKRLLFKPEEFEAVHSHTFRLKTFKKGKHCGVCKQTVNNEGLICRVCRLACHRKCEVKVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAEYTARIRCINDSQSAPEFGHRRRSLSFSGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGEVLPQTPAFPVSPQTPYFNMCRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAGQKSSADGHACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_021479028.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479028.1

ORF Insert Sequence:

ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA 
GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT 
TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT 
CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC 
CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG 
AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG 
AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT 
AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC 
AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG 
CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG 
ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG 
GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT 
TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT 
TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC 
AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC 
ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC 
TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG 
ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG 
TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC 
ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT 
AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA 
AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC 
CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC 
ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT 
AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC 
ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT 
GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG 
CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG 
GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT 
GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC 
AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC 
CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT 
CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA 
GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA 
GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG 
TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA 
ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA 
GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG 
TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG 
ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA 
GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC 
CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC 
TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT 
ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC 
ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA 
AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA 
TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA 
GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT 
CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG 
TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA 
GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG 
CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC 
TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT 
GGTGAGGTCC TACCCCAAAC CCCTGCCTTC CCTGTCTCGC CACAAACCCC TTACTTCAAC 
ATGTGTCGAT CTCCTCCAGG ATTGGCTAAA ACACCACTGT CCTTTCTGGG CCTGAAGCCC 
CACAACCCTG CTGAGATCCT GCTCAACCAG ACAGGCTCAG AGCCTCGTAG TTATGTGGAG 
TCTGTGGCGA AGGCAGCAGC AGTACCTGCA TCTCCAACAT ATGACAGCGA CAGCTTAGCT 
CGTCCTGCCG GACCGTCCCA TGCTTACAAT GCTCCACTTT CCTCCAGCAG CCCCATTCAG 
AACCCAGATC CAGTTGAAAG CACATTGGCT GACAGCGGGG TCGGGATGAA TTCTTTATCC 
CAGGCTGCTG TCGACATTTT CCATTTCCAG CCCTCCGAAA TCCCATACCA ACCACCACCA 
CCCCCCTACC CAACCTCCAT GTTGGGTAGC TACATCAGCC CTTCTCCAGC AGAGGCCATG 
CAAAATAATA CCACCACCAT CCAGCCACTT TCTGGAAGTC CTGGGACAAG ACACCACACC 
ATCCTTTCTG ACATGGCGTC CGTTCCCAAC TTTCAACACA GATTAGCAAA TCAGGATGGT 
CCCATACTTG GGCGACAGCA GGCTCCAGCT AATGGTCATG TGCCTGGAAT TGCTGGTAGT 
CCAGTGCAAG GAAGACACAC TATGGTGTCC CAGGGTACAC AAAGCAGTCC CATACTGAGC 
AGACAGTATC CTGTCACCCA GGGGATTCAG AGTAGTCCAA TTCTCAGCAG GCAGCCAATG 
GGACAAGCTG TCCAGAGCAG TCCAGTCCTC AACCGACAGG CATCCCTGAA TCAAGCAAAC 
CAAAGCAGTC CAGTGTTGAG TAGACAGCAG TCCATTACCC TACCCAACCA AGGCAGCCCG 
GTCCTAAGCA GACAGTCCTC GCTTAGTCAT CCCAGTCAGG GTAGTCCTGT ACTAGGACGC 
CACCCTTCCC TAACACAGGG TAGCCCCAGC CTGGATAGGC ATCCGATGTA CAGCGGTTAC 
ACCACTCCAG AAGAGCGACA CGGAGCTCTG TCACGCCAAA GCAGCTCTTC AGGATACCAG 
CCGCCATCTA CACCGTCTTT CCCAGTTTCT CCAGCAGGAT ACATCGAGGG AGCGGGATTC 
AGACAGGGGA GTCCTGCTTT CCAACCTCAG TTGCCCGAAA AGAGACGCAT GTCCAGCAGT 
GACCGCACCA ATGGTGGCCT GTCCTATGGG ACGCTCAATG GGAAGATGTC CTCACCGGTG 
TCCAGTGGCG GCAGCACTCC GAGTGTTCAT TTCTTCCACA CACTCCCCGA TTTCTCCAAA 
CTCAACATGT GTGATGGAAG TCCAGAAACC AGAATGAACG TGAAGTTTGT CCAGGACACG 
TCCAAATTCT GGTATAAACC AGATATCTCG AGAGACCAAG CGATTAACAT GCTGAAGGAT 
CAAGATCCTG GGGCCTTTGT GATTCGGGAC AGTCATTCAT TCAGAGGAGC CTACGGCTTG 
GCTATGAAAG TGGCTTCACC ACCTCCAACC ACTCAACCTA CAAAGAAAGC TGGAGATGTC 
ACTAATGAGT TGGTGAGGCA TTTTCTAATT GAGACGAGTC CGAAGGGTGT GAGACTGAAG 
GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC CTGTCTGCGC TGGTCTACCA GCACTCCATC 
ACTCCACTGG CACTGCCCTG CAAACTAGTC ATACCAACCA GAGATCCTCT GGAAGAGTCA 
CCTGAGATCG CAACACCAAC CAACCCGGCG GCAGATATGC TCAAGCAGGG CGCAGGGCAG 
AAGAGTTCAG CTGATGGCCA TGCATGCAAT GTGCTGTACA TCAACTCTGT GGATATGGAG 
TCTTTGACTG GACCTCAAGC CATCGCCAAA GCCATTTCAG AAACCATGAA CGCCAGTCCG 
GCACCCTCAG CCACCATTGT TCACTTCAAG GTGTCGGCAC AAGGCATCAC ACTCACAGAC 
AACCAGAGAA AGCTTTTCTT CAGACGTCAT TATCCCATCG GCACAGTCAC GTTCTGTGAC 
ATTGATCCAC AGGAGAGGAA GTGGAACAAA CCAGAGGGTG GCACTGCCAA ATTCTTTGGC 
TTTGTGGCTA GAAAGCAAGG CAGCACGACA GACAATGTCA GTCACCTGTT TGCTGAGATG 
GACCCAGATC AGCCGGCCAC CGCCATCGTC AACTTTGTCT CTAAAGTGAT GATCAGCTCT 
CAGAAGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_021479029.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334704.1 Danio rerio tensin 1b (tns1b), transcript variant X3, mRNA.

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CloneID	ODa144115
Clone ID Related Accession (Same CDS sequence)	XM_021479029.1
Accession Version	XM_021479029.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG 
CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG 
GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG 
TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA 
CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG 
TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC 
ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC 
GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC 
TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT 
GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG 
GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA 
GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC 
GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG 
GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT 
AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC 
GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC 
ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA 
CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC 
CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT 
ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT 
GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT 
GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT 
TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA 
GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG 
GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT 
CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC 
ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT 
TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA 
GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT 
GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA 
GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT 
CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA 
GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG 
CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG 
TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA 
GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG 
AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC 
TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA 
GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT 
ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA 
CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC 
CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT 
TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT 
TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA 
GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG 
CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA 
CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGAGTACACT 
GCTCGTATCC GCTGCATCAA TGACAGCCAG AGTGCTCCCG AGTTTGGCCA TCGCCGCCGC 
TCTCTCTCCT TCAGTGGACT TCCATCTGGT GAAATGTCGC CAGATGAGGC TGCAGTTCCT 
GGACGCTGTC GGACCCTTAG TGAGGGCCTT TCCCAAGACG AAAGCCCAAC TCGAGGAAGC 
TCAGTCCGCT CCCCCATTCG CTGCATCTCA CCAGAGCTGG CCAATACAAT TGCTCTGAAC 
CCTGGGGGGC GGCCCAAGGA GAGGCACATG CACAGCTACA GAGAAGCGTT TGAGGAAATG 
GATGGGGGTC CCGTTAGCCC CCCTCCAATT GTTGGTGGTG AGGTCCTACC CCAAACCCCT 
GCCTTCCCTG TCTCGCCACA AACCCCTTAC TTCAACATGT GTCGATCTCC TCCAGGATTG 
GCTAAAACAC CACTGTCCTT TCTGGGCCTG AAGCCCCACA ACCCTGCTGA GATCCTGCTC 
AACCAGACAG GCTCAGAGAG TGAGGATGAG GAAGAGCCTC GTAGTTATGT GGAGTCTGTG 
GCGAAGGCAG CAGCAGTACC TGCATCTCCA ACATATGACA GCGACAGCTT AGCTCGTCCT 
GCCGGACCGT CCCATGCTTA CAATGCTCCA CTTTCCTCCA GCAGCCCCAT TCAGAACCCA 
GATCCAGTTG AAAGCACATT GGCTGACAGC GGGGTCGGGA TGAATTCTTT ATCCCAGGCT 
GCTGTCGACA TTTTCCATTT CCAGCCCTCC GAAATCCCAT ACCAACCACC ACCACCCCCC 
TACCCAACCT CCATGTTGGG TAGCTACATC AGCCCTTCTC CAGCAGAGGC CATGCAAAAT 
AATACCACCA CCATCCAGCC ACTTTCTGGA AGTCCTGGGA CAAGACACCA CACCATCCTT 
TCTGACATGG CGTCCGTTCC CAACTTTCAA CACAGATTAG CAAATCAGGA TGGTCCCATA 
CTTGGGCGAC AGCAGGCTCC AGCTAATGGT CATGTGCCTG GAATTGCTGG TAGTCCAGTG 
CAAGGAAGAC ACACTATGGT GTCCCAGGGT ACACAAAGCA GTCCCATACT GAGCAGACAG 
TATCCTGTCA CCCAGGGGAT TCAGAGTAGT CCAATTCTCA GCAGGCAGCC AATGGGACAA 
GCTGTCCAGA GCAGTCCAGT CCTCAACCGA CAGGCATCCC TGAATCAAGC AAACCAAAGC 
AGTCCAGTGT TGAGTAGACA GCAGTCCATT ACCCTACCCA ACCAAGGCAG CCCGGTCCTA 
AGCAGACAGT CCTCGCTTAG TCATCCCAGT CAGGGTAGTC CTGTACTAGG ACGCCACCCT 
TCCCTAACAC AGGGTAGCCC CAGCCTGGAT AGGCATCCGA TGTACAGCGG TTACACCACT 
CCAGAAGAGC GACACGGAGC TCTGTCACGC CAAAGCAGCT CTTCAGGATA CCAGCCGCCA 
TCTACACCGT CTTTCCCAGT TTCTCCAGCA GGATACATCG AGGGAGCGGG ATTCAGACAG 
GGGAGTCCTG CTTTCCAACC TCAGTTGCCC GAAAAGAGAC GCATGTCCAG CAGTGACCGC 
ACCAATGGTG GCCTGTCCTA TGGGACGCTC AATGGGAAGA TGTCCTCACC GGTGTCCAGT 
GGCGGCAGCA CTCCGAGTGT TCATTTCTTC CACACACTCC CCGATTTCTC CAAACTCAAC 
ATGTGTGATG GAAGTCCAGA AACCAGAATG AACGTGAAGT TTGTCCAGGA CACGTCCAAA 
TTCTGGTATA AACCAGATAT CTCGAGAGAC CAAGCGATTA ACATGCTGAA GGATCAAGAT 
CCTGGGGCCT TTGTGATTCG GGACAGTCAT TCATTCAGAG GAGCCTACGG CTTGGCTATG 
AAAGTGGCTT CACCACCTCC AACCACTCAA CCTACAAAGA AAGCTGGAGA TGTCACTAAT 
GAGTTGGTGA GGCATTTTCT AATTGAGACG AGTCCGAAGG GTGTGAGACT GAAGGGCTGC 
CCCAACGAGC CGTACTTTGG CTGCCTGTCT GCGCTGGTCT ACCAGCACTC CATCACTCCA 
CTGGCACTGC CCTGCAAACT AGTCATACCA ACCAGAGATC CTCTGGAAGA GTCACCTGAG 
ATCGCAACAC CAACCAACCC GGCGGCAGAT ATGCTCAAGC AGGGCGCAGG GCAGAAGAGT 
TCAGCTGATG GCCATGCATG CAATGTGCTG TACATCAACT CTGTGGATAT GGAGTCTTTG 
ACTGGACCTC AAGCCATCGC CAAAGCCATT TCAGAAACCA TGAACGCCAG TCCGGCACCC 
TCAGCCACCA TTGTTCACTT CAAGGTGTCG GCACAAGGCA TCACACTCAC AGACAACCAG 
AGAAAGCTTT TCTTCAGACG TCATTATCCC ATCGGCACAG TCACGTTCTG TGACATTGAT 
CCACAGGAGA GGAAGTGGAA CAAACCAGAG GGTGGCACTG CCAAATTCTT TGGCTTTGTG 
GCTAGAAAGC AAGGCAGCAC GACAGACAAT GTCAGTCACC TGTTTGCTGA GATGGACCCA 
GATCAGCCGG CCACCGCCAT CGTCAACTTT GTCTCTAAAG TGATGATCAG CTCTCAGAAG 
CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334704.1
CDS	815..6280
Translation	MPSVSLSLPAALAGRARTWVCLSCMFWPEEFEAVHSHTFRLKTFKKGKHCGVCKQTVNNEGLICRVCRLACHRKCEVKVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAEYTARIRCINDSQSAPEFGHRRRSLSFSGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGEVLPQTPAFPVSPQTPYFNMCRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSESEDEEEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAGQKSSADGHACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_021479029.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479029.1

ORF Insert Sequence:

ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG 
CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG 
GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG 
TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA 
CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG 
TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC 
ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC 
GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC 
TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT 
GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG 
GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA 
GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC 
GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG 
GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT 
AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC 
GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC 
ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA 
CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC 
CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT 
ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT 
GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT 
GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT 
TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA 
GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG 
GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT 
CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC 
ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT 
TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA 
GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT 
GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA 
GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT 
CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA 
GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG 
CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG 
TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA 
GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG 
AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC 
TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA 
GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT 
ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA 
CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC 
CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT 
TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT 
TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA 
GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG 
CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA 
CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGAGTACACT 
GCTCGTATCC GCTGCATCAA TGACAGCCAG AGTGCTCCCG AGTTTGGCCA TCGCCGCCGC 
TCTCTCTCCT TCAGTGGACT TCCATCTGGT GAAATGTCGC CAGATGAGGC TGCAGTTCCT 
GGACGCTGTC GGACCCTTAG TGAGGGCCTT TCCCAAGACG AAAGCCCAAC TCGAGGAAGC 
TCAGTCCGCT CCCCCATTCG CTGCATCTCA CCAGAGCTGG CCAATACAAT TGCTCTGAAC 
CCTGGGGGGC GGCCCAAGGA GAGGCACATG CACAGCTACA GAGAAGCGTT TGAGGAAATG 
GATGGGGGTC CCGTTAGCCC CCCTCCAATT GTTGGTGGTG AGGTCCTACC CCAAACCCCT 
GCCTTCCCTG TCTCGCCACA AACCCCTTAC TTCAACATGT GTCGATCTCC TCCAGGATTG 
GCTAAAACAC CACTGTCCTT TCTGGGCCTG AAGCCCCACA ACCCTGCTGA GATCCTGCTC 
AACCAGACAG GCTCAGAGAG TGAGGATGAG GAAGAGCCTC GTAGTTATGT GGAGTCTGTG 
GCGAAGGCAG CAGCAGTACC TGCATCTCCA ACATATGACA GCGACAGCTT AGCTCGTCCT 
GCCGGACCGT CCCATGCTTA CAATGCTCCA CTTTCCTCCA GCAGCCCCAT TCAGAACCCA 
GATCCAGTTG AAAGCACATT GGCTGACAGC GGGGTCGGGA TGAATTCTTT ATCCCAGGCT 
GCTGTCGACA TTTTCCATTT CCAGCCCTCC GAAATCCCAT ACCAACCACC ACCACCCCCC 
TACCCAACCT CCATGTTGGG TAGCTACATC AGCCCTTCTC CAGCAGAGGC CATGCAAAAT 
AATACCACCA CCATCCAGCC ACTTTCTGGA AGTCCTGGGA CAAGACACCA CACCATCCTT 
TCTGACATGG CGTCCGTTCC CAACTTTCAA CACAGATTAG CAAATCAGGA TGGTCCCATA 
CTTGGGCGAC AGCAGGCTCC AGCTAATGGT CATGTGCCTG GAATTGCTGG TAGTCCAGTG 
CAAGGAAGAC ACACTATGGT GTCCCAGGGT ACACAAAGCA GTCCCATACT GAGCAGACAG 
TATCCTGTCA CCCAGGGGAT TCAGAGTAGT CCAATTCTCA GCAGGCAGCC AATGGGACAA 
GCTGTCCAGA GCAGTCCAGT CCTCAACCGA CAGGCATCCC TGAATCAAGC AAACCAAAGC 
AGTCCAGTGT TGAGTAGACA GCAGTCCATT ACCCTACCCA ACCAAGGCAG CCCGGTCCTA 
AGCAGACAGT CCTCGCTTAG TCATCCCAGT CAGGGTAGTC CTGTACTAGG ACGCCACCCT 
TCCCTAACAC AGGGTAGCCC CAGCCTGGAT AGGCATCCGA TGTACAGCGG TTACACCACT 
CCAGAAGAGC GACACGGAGC TCTGTCACGC CAAAGCAGCT CTTCAGGATA CCAGCCGCCA 
TCTACACCGT CTTTCCCAGT TTCTCCAGCA GGATACATCG AGGGAGCGGG ATTCAGACAG 
GGGAGTCCTG CTTTCCAACC TCAGTTGCCC GAAAAGAGAC GCATGTCCAG CAGTGACCGC 
ACCAATGGTG GCCTGTCCTA TGGGACGCTC AATGGGAAGA TGTCCTCACC GGTGTCCAGT 
GGCGGCAGCA CTCCGAGTGT TCATTTCTTC CACACACTCC CCGATTTCTC CAAACTCAAC 
ATGTGTGATG GAAGTCCAGA AACCAGAATG AACGTGAAGT TTGTCCAGGA CACGTCCAAA 
TTCTGGTATA AACCAGATAT CTCGAGAGAC CAAGCGATTA ACATGCTGAA GGATCAAGAT 
CCTGGGGCCT TTGTGATTCG GGACAGTCAT TCATTCAGAG GAGCCTACGG CTTGGCTATG 
AAAGTGGCTT CACCACCTCC AACCACTCAA CCTACAAAGA AAGCTGGAGA TGTCACTAAT 
GAGTTGGTGA GGCATTTTCT AATTGAGACG AGTCCGAAGG GTGTGAGACT GAAGGGCTGC 
CCCAACGAGC CGTACTTTGG CTGCCTGTCT GCGCTGGTCT ACCAGCACTC CATCACTCCA 
CTGGCACTGC CCTGCAAACT AGTCATACCA ACCAGAGATC CTCTGGAAGA GTCACCTGAG 
ATCGCAACAC CAACCAACCC GGCGGCAGAT ATGCTCAAGC AGGGCGCAGG GCAGAAGAGT 
TCAGCTGATG GCCATGCATG CAATGTGCTG TACATCAACT CTGTGGATAT GGAGTCTTTG 
ACTGGACCTC AAGCCATCGC CAAAGCCATT TCAGAAACCA TGAACGCCAG TCCGGCACCC 
TCAGCCACCA TTGTTCACTT CAAGGTGTCG GCACAAGGCA TCACACTCAC AGACAACCAG 
AGAAAGCTTT TCTTCAGACG TCATTATCCC ATCGGCACAG TCACGTTCTG TGACATTGAT 
CCACAGGAGA GGAAGTGGAA CAAACCAGAG GGTGGCACTG CCAAATTCTT TGGCTTTGTG 
GCTAGAAAGC AAGGCAGCAC GACAGACAAT GTCAGTCACC TGTTTGCTGA GATGGACCCA 
GATCAGCCGG CCACCGCCAT CGTCAACTTT GTCTCTAAAG TGATGATCAG CTCTCAGAAG 
CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_021479030.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334705.1 Danio rerio tensin 1b (tns1b), transcript variant X4, mRNA.

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CloneID	ODa144116
Clone ID Related Accession (Same CDS sequence)	XM_021479030.1
Accession Version	XM_021479030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGAGT CCAGTTCTTC TTTGGGATTA AATATTCGGG GGCTAACAAG ACGAGTCGGT 
GAGATGATGT CCGGCTGGAC GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG 
CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG 
GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG 
TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA 
CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG 
TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC 
ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC 
GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC 
TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT 
GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG 
GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA 
GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC 
GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG 
GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT 
AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC 
GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC 
ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA 
CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC 
CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT 
ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT 
GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT 
GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT 
TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA 
GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG 
GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT 
CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC 
ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT 
TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA 
GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT 
GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA 
GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT 
CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA 
GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG 
CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG 
TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA 
GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG 
AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC 
TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA 
GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT 
ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA 
CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC 
CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT 
TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT 
TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA 
GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG 
CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA 
CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGAGTACACT 
GCTCGTATCC GCTGCATCAA TGACAGCCAG AGTGCTCCCG AGTTTGGCCA TCGCCGCCGC 
TCTCTCTCCT TCAGTGGACT TCCATCTGGT GAAATGTCGC CAGATGAGGC TGCAGTTCCT 
GGACGCTGTC GGACCCTTAG TGAGGGCCTT TCCCAAGACG AAAGCCCAAC TCGAGGAAGC 
TCAGTCCGCT CCCCCATTCG CTGCATCTCA CCAGAGCTGG CCAATACAAT TGCTCTGAAC 
CCTGGGGGGC GGCCCAAGGA GAGGCACATG CACAGCTACA GAGAAGCGTT TGAGGAAATG 
GATGGGGGTC CCGTTAGCCC CCCTCCAATT GTTGGTGGTG AGGTCCTACC CCAAACCCCT 
GCCTTCCCTG TCTCGCCACA AACCCCTTAC TTCAACATGT GTCGATCTCC TCCAGGATTG 
GCTAAAACAC CACTGTCCTT TCTGGGCCTG AAGCCCCACA ACCCTGCTGA GATCCTGCTC 
AACCAGACAG GCTCAGAGAG TGAGGATGAG GAAGAGCCTC GTAGTTATGT GGAGTCTGTG 
GCGAAGGCAG CAGCAGTACC TGCATCTCCA ACATATGACA GCGACAGCTT AGCTCGTCCT 
GCCGGACCGT CCCATGCTTA CAATGCTCCA CTTTCCTCCA GCAGCCCCAT TCAGAACCCA 
GATCCAGTTG AAAGCACATT GGCTGACAGC GGGGTCGGGA TGAATTCTTT ATCCCAGGCT 
GCTGTCGACA TTTTCCATTT CCAGCCCTCC GAAATCCCAT ACCAACCACC ACCACCCCCC 
TACCCAACCT CCATGTTGGG TAGCTACATC AGCCCTTCTC CAGCAGAGGC CATGCAAAAT 
AATACCACCA CCATCCAGCC ACTTTCTGGA AGTCCTGGGA CAAGACACCA CACCATCCTT 
TCTGACATGG CGTCCGTTCC CAACTTTCAA CACAGATTAG CAAATCAGGA TGGTCCCATA 
CTTGGGCGAC AGCAGGCTCC AGCTAATGGT CATGTGCCTG GAATTGCTGG TAGTCCAGTG 
CAAGGAAGAC ACACTATGGT GTCCCAGGGT ACACAAAGCA GTCCCATACT GAGCAGACAG 
TATCCTGTCA CCCAGGGGAT TCAGAGTAGT CCAATTCTCA GCAGGCAGCC AATGGGACAA 
GCTGTCCAGA GCAGTCCAGT CCTCAACCGA CAGGCATCCC TGAATCAAGC AAACCAAAGC 
AGTCCAGTGT TGAGTAGACA GCAGTCCATT ACCCTACCCA ACCAAGGCAG CCCGGTCCTA 
AGCAGACAGT CCTCGCTTAG TCATCCCAGT CAGGGTAGTC CTGTACTAGG ACGCCACCCT 
TCCCTAACAC AGGGTAGCCC CAGCCTGGAT AGGCATCCGA TGTACAGCGG TTACACCACT 
CCAGAAGAGC GACACGGAGC TCTGTCACGC CAAAGCAGCT CTTCAGGATA CCAGCCGCCA 
TCTACACCGT CTTTCCCAGT TTCTCCAGCA GGATACATCG AGGGAGCGGG ATTCAGACAG 
GGGAGTCCTG CTTTCCAACC TCAGTTGCCC GAAAAGAGAC GCATGTCCAG CAGTGACCGC 
ACCAATGGTG GCCTGTCCTA TGGGACGCTC AATGGGAAGA TGTCCTCACC GGTGTCCAGT 
GGCGGCAGCA CTCCGAGTGT TCATTTCTTC CACACACTCC CCGATTTCTC CAAACTCAAC 
ATGTGTGATG GAAGTCCAGA AACCAGAATG AACGTGAAGT TTGTCCAGGA CACGTCCAAA 
TTCTGGTATA AACCAGATAT CTCGAGAGAC CAAGCGATTA ACATGCTGAA GGATCAAGAT 
CCTGGGGCCT TTGTGATTCG GGACAGTCAT TCATTCAGAG GAGCCTACGG CTTGGCTATG 
AAAGTGGCTT CACCACCTCC AACCACTCAA CCTACAAAGA AAGCTGGAGA TGTCACTAAT 
GAGTTGGTGA GGCATTTTCT AATTGAGACG AGTCCGAAGG GTGTGAGACT GAAGGGCTGC 
CCCAACGAGC CGTACTTTGG CTGCCTGTCT GCGCTGGTCT ACCAGCACTC CATCACTCCA 
CTGGCACTGC CCTGCAAACT AGTCATACCA ACCAGAGATC CTCTGGAAGA GTCACCTGAG 
ATCGCAACAC CAACCAACCC GGCGGCAGAT ATGCTCAAGC AGGGCGCAGG GCAGAAGAGT 
TCAGCTGATG GCCATGCATG CAATGTGCTG TACATCAACT CTGTGGATAT GGAGTCTTTG 
ACTGGACCTC AAGCCATCGC CAAAGCCATT TCAGAAACCA TGAACGCCAG TCCGGCACCC 
TCAGCCACCA TTGTTCACTT CAAGGTGTCG GCACAAGGCA TCACACTCAC AGACAACCAG 
AGAAAGCTTT TCTTCAGACG TCATTATCCC ATCGGCACAG TCACGTTCTG TGACATTGAT 
CCACAGGAGA GGAAGTGGAA CAAACCAGAG GGTGGCACTG CCAAATTCTT TGGCTTTGTG 
GCTAGAAAGC AAGGCAGCAC GACAGACAAT GTCAGTCACC TGTTTGCTGA GATGGACCCA 
GATCAGCCGG CCACCGCCAT CGTCAACTTT GTCTCTAAAG TGATGATCAG CTCTCAGAAG 
CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334705.1
CDS	222..5687
Translation	MLESSSSLGLNIRGLTRRVGEMMSGWTPEEFEAVHSHTFRLKTFKKGKHCGVCKQTVNNEGLICRVCRLACHRKCEVKVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAEYTARIRCINDSQSAPEFGHRRRSLSFSGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGEVLPQTPAFPVSPQTPYFNMCRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSESEDEEEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAGQKSSADGHACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_021479030.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479030.1

ORF Insert Sequence:

ATGCTTGAGT CCAGTTCTTC TTTGGGATTA AATATTCGGG GGCTAACAAG ACGAGTCGGT 
GAGATGATGT CCGGCTGGAC GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG 
CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG 
GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG 
TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA 
CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG 
TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC 
ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC 
GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC 
TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT 
GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG 
GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA 
GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC 
GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG 
GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT 
AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC 
GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC 
ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA 
CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC 
CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT 
ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT 
GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT 
GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT 
TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA 
GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG 
GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT 
CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC 
ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT 
TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA 
GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT 
GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA 
GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT 
CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA 
GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG 
CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG 
TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA 
GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG 
AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC 
TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA 
GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT 
ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA 
CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC 
CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT 
TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT 
TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA 
GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG 
CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA 
CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGAGTACACT 
GCTCGTATCC GCTGCATCAA TGACAGCCAG AGTGCTCCCG AGTTTGGCCA TCGCCGCCGC 
TCTCTCTCCT TCAGTGGACT TCCATCTGGT GAAATGTCGC CAGATGAGGC TGCAGTTCCT 
GGACGCTGTC GGACCCTTAG TGAGGGCCTT TCCCAAGACG AAAGCCCAAC TCGAGGAAGC 
TCAGTCCGCT CCCCCATTCG CTGCATCTCA CCAGAGCTGG CCAATACAAT TGCTCTGAAC 
CCTGGGGGGC GGCCCAAGGA GAGGCACATG CACAGCTACA GAGAAGCGTT TGAGGAAATG 
GATGGGGGTC CCGTTAGCCC CCCTCCAATT GTTGGTGGTG AGGTCCTACC CCAAACCCCT 
GCCTTCCCTG TCTCGCCACA AACCCCTTAC TTCAACATGT GTCGATCTCC TCCAGGATTG 
GCTAAAACAC CACTGTCCTT TCTGGGCCTG AAGCCCCACA ACCCTGCTGA GATCCTGCTC 
AACCAGACAG GCTCAGAGAG TGAGGATGAG GAAGAGCCTC GTAGTTATGT GGAGTCTGTG 
GCGAAGGCAG CAGCAGTACC TGCATCTCCA ACATATGACA GCGACAGCTT AGCTCGTCCT 
GCCGGACCGT CCCATGCTTA CAATGCTCCA CTTTCCTCCA GCAGCCCCAT TCAGAACCCA 
GATCCAGTTG AAAGCACATT GGCTGACAGC GGGGTCGGGA TGAATTCTTT ATCCCAGGCT 
GCTGTCGACA TTTTCCATTT CCAGCCCTCC GAAATCCCAT ACCAACCACC ACCACCCCCC 
TACCCAACCT CCATGTTGGG TAGCTACATC AGCCCTTCTC CAGCAGAGGC CATGCAAAAT 
AATACCACCA CCATCCAGCC ACTTTCTGGA AGTCCTGGGA CAAGACACCA CACCATCCTT 
TCTGACATGG CGTCCGTTCC CAACTTTCAA CACAGATTAG CAAATCAGGA TGGTCCCATA 
CTTGGGCGAC AGCAGGCTCC AGCTAATGGT CATGTGCCTG GAATTGCTGG TAGTCCAGTG 
CAAGGAAGAC ACACTATGGT GTCCCAGGGT ACACAAAGCA GTCCCATACT GAGCAGACAG 
TATCCTGTCA CCCAGGGGAT TCAGAGTAGT CCAATTCTCA GCAGGCAGCC AATGGGACAA 
GCTGTCCAGA GCAGTCCAGT CCTCAACCGA CAGGCATCCC TGAATCAAGC AAACCAAAGC 
AGTCCAGTGT TGAGTAGACA GCAGTCCATT ACCCTACCCA ACCAAGGCAG CCCGGTCCTA 
AGCAGACAGT CCTCGCTTAG TCATCCCAGT CAGGGTAGTC CTGTACTAGG ACGCCACCCT 
TCCCTAACAC AGGGTAGCCC CAGCCTGGAT AGGCATCCGA TGTACAGCGG TTACACCACT 
CCAGAAGAGC GACACGGAGC TCTGTCACGC CAAAGCAGCT CTTCAGGATA CCAGCCGCCA 
TCTACACCGT CTTTCCCAGT TTCTCCAGCA GGATACATCG AGGGAGCGGG ATTCAGACAG 
GGGAGTCCTG CTTTCCAACC TCAGTTGCCC GAAAAGAGAC GCATGTCCAG CAGTGACCGC 
ACCAATGGTG GCCTGTCCTA TGGGACGCTC AATGGGAAGA TGTCCTCACC GGTGTCCAGT 
GGCGGCAGCA CTCCGAGTGT TCATTTCTTC CACACACTCC CCGATTTCTC CAAACTCAAC 
ATGTGTGATG GAAGTCCAGA AACCAGAATG AACGTGAAGT TTGTCCAGGA CACGTCCAAA 
TTCTGGTATA AACCAGATAT CTCGAGAGAC CAAGCGATTA ACATGCTGAA GGATCAAGAT 
CCTGGGGCCT TTGTGATTCG GGACAGTCAT TCATTCAGAG GAGCCTACGG CTTGGCTATG 
AAAGTGGCTT CACCACCTCC AACCACTCAA CCTACAAAGA AAGCTGGAGA TGTCACTAAT 
GAGTTGGTGA GGCATTTTCT AATTGAGACG AGTCCGAAGG GTGTGAGACT GAAGGGCTGC 
CCCAACGAGC CGTACTTTGG CTGCCTGTCT GCGCTGGTCT ACCAGCACTC CATCACTCCA 
CTGGCACTGC CCTGCAAACT AGTCATACCA ACCAGAGATC CTCTGGAAGA GTCACCTGAG 
ATCGCAACAC CAACCAACCC GGCGGCAGAT ATGCTCAAGC AGGGCGCAGG GCAGAAGAGT 
TCAGCTGATG GCCATGCATG CAATGTGCTG TACATCAACT CTGTGGATAT GGAGTCTTTG 
ACTGGACCTC AAGCCATCGC CAAAGCCATT TCAGAAACCA TGAACGCCAG TCCGGCACCC 
TCAGCCACCA TTGTTCACTT CAAGGTGTCG GCACAAGGCA TCACACTCAC AGACAACCAG 
AGAAAGCTTT TCTTCAGACG TCATTATCCC ATCGGCACAG TCACGTTCTG TGACATTGAT 
CCACAGGAGA GGAAGTGGAA CAAACCAGAG GGTGGCACTG CCAAATTCTT TGGCTTTGTG 
GCTAGAAAGC AAGGCAGCAC GACAGACAAT GTCAGTCACC TGTTTGCTGA GATGGACCCA 
GATCAGCCGG CCACCGCCAT CGTCAACTTT GTCTCTAAAG TGATGATCAG CTCTCAGAAG 
CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_021479031.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334706.1 Danio rerio tensin 1b (tns1b), transcript variant X5, mRNA.

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CloneID	ODa144117
Clone ID Related Accession (Same CDS sequence)	XM_021479031.1
Accession Version	XM_021479031.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA 
GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT 
TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT 
CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC 
CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG 
AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG 
AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT 
AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC 
AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG 
CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG 
ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG 
GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT 
TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT 
TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC 
AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC 
ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC 
TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG 
ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG 
TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC 
ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT 
AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA 
AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC 
CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC 
ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT 
AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC 
ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT 
GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG 
CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG 
GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT 
GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC 
AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC 
CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT 
CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA 
GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA 
GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG 
TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA 
ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA 
GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG 
TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG 
ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA 
GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC 
CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC 
TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT 
ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC 
ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA 
AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA 
TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA 
GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT 
CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG 
TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA 
GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG 
CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC 
TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT 
GGTGAGGTCC TACCCCAAAC CCCTGCCTTC CCTGTCTCGC CACAAACCCC TTACTTCAAC 
ATGTGTCGAT CTCCTCCAGG ATTGGCTAAA ACACCACTGT CCTTTCTGGG CCTGAAGCCC 
CACAACCCTG CTGAGATCCT GCTCAACCAG ACAGGCTCAG AGAGTGAGGA TGAGGAAGAG 
CCTCGTAGTT ATGTGGAGTC TGTGGCGAAG GCAGCAGCAG TACCTGCATC TCCAACATAT 
GACAGCGACA GCTTAGCTCG TCCTGCCGGA CCGTCCCATG CTTACAATGC TCCACTTTCC 
TCCAGCAGCC CCATTCAGAA CCCAGATCCA GTTGAAAGCA CATTGGCTGA CAGCGGGGTC 
GGGATGAATT CTTTATCCCA GGCTGCTGTC GACATTTTCC ATTTCCAGCC CTCCGAAATC 
CCATACCAAC CACCACCACC CCCCTACCCA ACCTCCATGT TGGGTAGCTA CATCAGCCCT 
TCTCCAGCAG AGGCCATGCA AAATAATACC ACCACCATCC AGCCACTTTC TGGAAGTCCT 
GGGACAAGAC ACCACACCAT CCTTTCTGAC ATGGCGTCCG TTCCCAACTT TCAACACAGA 
TTAGCAAATC AGGATGGTCC CATACTTGGG CGACAGCAGG CTCCAGCTAA TGGTCATGTG 
CCTGGAATTG CTGGTAGTCC AGTGCAAGGA AGACACACTA TGGTGTCCCA GGGTACACAA 
AGCAGTCCCA TACTGAGCAG ACAGTATCCT GTCACCCAGG GGATTCAGAG TAGTCCAATT 
CTCAGCAGGC AGCCAATGGG ACAAGCTGTC CAGAGCAGTC CAGTCCTCAA CCGACAGGCA 
TCCCTGAATC AAGCAAACCA AAGCAGTCCA GTGTTGAGTA GACAGCAGTC CATTACCCTA 
CCCAACCAAG GCAGCCCGGT CCTAAGCAGA CAGTCCTCGC TTAGTCATCC CAGTCAGGGT 
AGTCCTGTAC TAGGACGCCA CCCTTCCCTA ACACAGGGTA GCCCCAGCCT GGATAGGCAT 
CCGATGTACA GCGGTTACAC CACTCCAGAA GAGCGACACG GAGCTCTGTC ACGCCAAAGC 
AGCTCTTCAG GATACCAGCC GCCATCTACA CCGTCTTTCC CAGTTTCTCC AGCAGGATAC 
ATCGAGGGAG CGGGATTCAG ACAGGGGAGT CCTGCTTTCC AACCTCAGTT GCCCGAAAAG 
AGACGCATGT CCAGCAGTGA CCGCACCAAT GGTGGCCTGT CCTATGGGAC GCTCAATGGG 
AAGATGTCCT CACCGGTGTC CAGTGGCGGC AGCACTCCGA GTGTTCATTT CTTCCACACA 
CTCCCCGATT TCTCCAAACT CAACATGTGT GATGGAAGTC CAGAAACCAG AATGAACGTG 
AAGTTTGTCC AGGACACGTC CAAATTCTGG TATAAACCAG ATATCTCGAG AGACCAAGCG 
ATTAACATGC TGAAGGATCA AGATCCTGGG GCCTTTGTGA TTCGGGACAG TCATTCATTC 
AGAGGAGCCT ACGGCTTGGC TATGAAAGTG GCTTCACCAC CTCCAACCAC TCAACCTACA 
AAGAAAGCTG GAGATGTCAC TAATGAGTTG GTGAGGCATT TTCTAATTGA GACGAGTCCG 
AAGGGTGTGA GACTGAAGGG CTGCCCCAAC GAGCCGTACT TTGGCTGCCT GTCTGCGCTG 
GTCTACCAGC ACTCCATCAC TCCACTGGCA CTGCCCTGCA AACTAGTCAT ACCAACCAGA 
GATCCTCTGG AAGAGTCACC TGAGATCGCA ACACCAACCA ACCCGGCGGC AGATATGCTC 
AAGCAGGGCG CAGCATGCAA TGTGCTGTAC ATCAACTCTG TGGATATGGA GTCTTTGACT 
GGACCTCAAG CCATCGCCAA AGCCATTTCA GAAACCATGA ACGCCAGTCC GGCACCCTCA 
GCCACCATTG TTCACTTCAA GGTGTCGGCA CAAGGCATCA CACTCACAGA CAACCAGAGA 
AAGCTTTTCT TCAGACGTCA TTATCCCATC GGCACAGTCA CGTTCTGTGA CATTGATCCA 
CAGGAGAGGA AGTGGAACAA ACCAGAGGGT GGCACTGCCA AATTCTTTGG CTTTGTGGCT 
AGAAAGCAAG GCAGCACGAC AGACAATGTC AGTCACCTGT TTGCTGAGAT GGACCCAGAT 
CAGCCGGCCA CCGCCATCGT CAACTTTGTC TCTAAAGTGA TGATCAGCTC TCAGAAGCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334706.1
CDS	284..5746
Translation	MGCTVSFICCEDDFLQMPLDQHVEKKKEEKRLLFKPEEFEAVHSHTFRLKTFKKGKHCGVCKQTVNNEGLICRVCRLACHRKCEVKVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAEYTARIRCINDSQSAPEFGHRRRSLSFSGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGEVLPQTPAFPVSPQTPYFNMCRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSESEDEEEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_021479031.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479031.1

ORF Insert Sequence:

ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA 
GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT 
TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT 
CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC 
CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG 
AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG 
AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT 
AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC 
AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG 
CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG 
ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG 
GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT 
TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT 
TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC 
AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC 
ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC 
TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG 
ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG 
TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC 
ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT 
AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA 
AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC 
CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC 
ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT 
AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC 
ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT 
GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG 
CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG 
GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT 
GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC 
AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC 
CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT 
CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA 
GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA 
GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG 
TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA 
ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA 
GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG 
TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG 
ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA 
GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC 
CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC 
TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT 
ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC 
ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA 
AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA 
TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA 
GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT 
CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG 
TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA 
GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG 
CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC 
TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT 
GGTGAGGTCC TACCCCAAAC CCCTGCCTTC CCTGTCTCGC CACAAACCCC TTACTTCAAC 
ATGTGTCGAT CTCCTCCAGG ATTGGCTAAA ACACCACTGT CCTTTCTGGG CCTGAAGCCC 
CACAACCCTG CTGAGATCCT GCTCAACCAG ACAGGCTCAG AGAGTGAGGA TGAGGAAGAG 
CCTCGTAGTT ATGTGGAGTC TGTGGCGAAG GCAGCAGCAG TACCTGCATC TCCAACATAT 
GACAGCGACA GCTTAGCTCG TCCTGCCGGA CCGTCCCATG CTTACAATGC TCCACTTTCC 
TCCAGCAGCC CCATTCAGAA CCCAGATCCA GTTGAAAGCA CATTGGCTGA CAGCGGGGTC 
GGGATGAATT CTTTATCCCA GGCTGCTGTC GACATTTTCC ATTTCCAGCC CTCCGAAATC 
CCATACCAAC CACCACCACC CCCCTACCCA ACCTCCATGT TGGGTAGCTA CATCAGCCCT 
TCTCCAGCAG AGGCCATGCA AAATAATACC ACCACCATCC AGCCACTTTC TGGAAGTCCT 
GGGACAAGAC ACCACACCAT CCTTTCTGAC ATGGCGTCCG TTCCCAACTT TCAACACAGA 
TTAGCAAATC AGGATGGTCC CATACTTGGG CGACAGCAGG CTCCAGCTAA TGGTCATGTG 
CCTGGAATTG CTGGTAGTCC AGTGCAAGGA AGACACACTA TGGTGTCCCA GGGTACACAA 
AGCAGTCCCA TACTGAGCAG ACAGTATCCT GTCACCCAGG GGATTCAGAG TAGTCCAATT 
CTCAGCAGGC AGCCAATGGG ACAAGCTGTC CAGAGCAGTC CAGTCCTCAA CCGACAGGCA 
TCCCTGAATC AAGCAAACCA AAGCAGTCCA GTGTTGAGTA GACAGCAGTC CATTACCCTA 
CCCAACCAAG GCAGCCCGGT CCTAAGCAGA CAGTCCTCGC TTAGTCATCC CAGTCAGGGT 
AGTCCTGTAC TAGGACGCCA CCCTTCCCTA ACACAGGGTA GCCCCAGCCT GGATAGGCAT 
CCGATGTACA GCGGTTACAC CACTCCAGAA GAGCGACACG GAGCTCTGTC ACGCCAAAGC 
AGCTCTTCAG GATACCAGCC GCCATCTACA CCGTCTTTCC CAGTTTCTCC AGCAGGATAC 
ATCGAGGGAG CGGGATTCAG ACAGGGGAGT CCTGCTTTCC AACCTCAGTT GCCCGAAAAG 
AGACGCATGT CCAGCAGTGA CCGCACCAAT GGTGGCCTGT CCTATGGGAC GCTCAATGGG 
AAGATGTCCT CACCGGTGTC CAGTGGCGGC AGCACTCCGA GTGTTCATTT CTTCCACACA 
CTCCCCGATT TCTCCAAACT CAACATGTGT GATGGAAGTC CAGAAACCAG AATGAACGTG 
AAGTTTGTCC AGGACACGTC CAAATTCTGG TATAAACCAG ATATCTCGAG AGACCAAGCG 
ATTAACATGC TGAAGGATCA AGATCCTGGG GCCTTTGTGA TTCGGGACAG TCATTCATTC 
AGAGGAGCCT ACGGCTTGGC TATGAAAGTG GCTTCACCAC CTCCAACCAC TCAACCTACA 
AAGAAAGCTG GAGATGTCAC TAATGAGTTG GTGAGGCATT TTCTAATTGA GACGAGTCCG 
AAGGGTGTGA GACTGAAGGG CTGCCCCAAC GAGCCGTACT TTGGCTGCCT GTCTGCGCTG 
GTCTACCAGC ACTCCATCAC TCCACTGGCA CTGCCCTGCA AACTAGTCAT ACCAACCAGA 
GATCCTCTGG AAGAGTCACC TGAGATCGCA ACACCAACCA ACCCGGCGGC AGATATGCTC 
AAGCAGGGCG CAGCATGCAA TGTGCTGTAC ATCAACTCTG TGGATATGGA GTCTTTGACT 
GGACCTCAAG CCATCGCCAA AGCCATTTCA GAAACCATGA ACGCCAGTCC GGCACCCTCA 
GCCACCATTG TTCACTTCAA GGTGTCGGCA CAAGGCATCA CACTCACAGA CAACCAGAGA 
AAGCTTTTCT TCAGACGTCA TTATCCCATC GGCACAGTCA CGTTCTGTGA CATTGATCCA 
CAGGAGAGGA AGTGGAACAA ACCAGAGGGT GGCACTGCCA AATTCTTTGG CTTTGTGGCT 
AGAAAGCAAG GCAGCACGAC AGACAATGTC AGTCACCTGT TTGCTGAGAT GGACCCAGAT 
CAGCCGGCCA CCGCCATCGT CAACTTTGTC TCTAAAGTGA TGATCAGCTC TCAGAAGCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_021479032.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334707.1 Danio rerio tensin 1b (tns1b), transcript variant X6, mRNA.

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CloneID	ODa144118
Clone ID Related Accession (Same CDS sequence)	XM_021479032.1
Accession Version	XM_021479032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA 
GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT 
TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT 
CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC 
CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG 
AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG 
AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT 
AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC 
AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG 
CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG 
ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG 
GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT 
TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT 
TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC 
AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC 
ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC 
TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG 
ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG 
TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC 
ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT 
AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA 
AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC 
CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC 
ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT 
AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC 
ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT 
GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG 
CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG 
GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT 
GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC 
AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC 
CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT 
CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA 
GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA 
GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG 
TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA 
ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA 
GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG 
TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG 
ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA 
GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC 
CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC 
TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT 
ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC 
ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA 
AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA 
TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA 
GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT 
CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG 
TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA 
GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG 
CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC 
TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT 
GGTCGATCTC CTCCAGGATT GGCTAAAACA CCACTGTCCT TTCTGGGCCT GAAGCCCCAC 
AACCCTGCTG AGATCCTGCT CAACCAGACA GGCTCAGAGA GTGAGGATGA GGAAGAGCCT 
CGTAGTTATG TGGAGTCTGT GGCGAAGGCA GCAGCAGTAC CTGCATCTCC AACATATGAC 
AGCGACAGCT TAGCTCGTCC TGCCGGACCG TCCCATGCTT ACAATGCTCC ACTTTCCTCC 
AGCAGCCCCA TTCAGAACCC AGATCCAGTT GAAAGCACAT TGGCTGACAG CGGGGTCGGG 
ATGAATTCTT TATCCCAGGC TGCTGTCGAC ATTTTCCATT TCCAGCCCTC CGAAATCCCA 
TACCAACCAC CACCACCCCC CTACCCAACC TCCATGTTGG GTAGCTACAT CAGCCCTTCT 
CCAGCAGAGG CCATGCAAAA TAATACCACC ACCATCCAGC CACTTTCTGG AAGTCCTGGG 
ACAAGACACC ACACCATCCT TTCTGACATG GCGTCCGTTC CCAACTTTCA ACACAGATTA 
GCAAATCAGG ATGGTCCCAT ACTTGGGCGA CAGCAGGCTC CAGCTAATGG TCATGTGCCT 
GGAATTGCTG GTAGTCCAGT GCAAGGAAGA CACACTATGG TGTCCCAGGG TACACAAAGC 
AGTCCCATAC TGAGCAGACA GTATCCTGTC ACCCAGGGGA TTCAGAGTAG TCCAATTCTC 
AGCAGGCAGC CAATGGGACA AGCTGTCCAG AGCAGTCCAG TCCTCAACCG ACAGGCATCC 
CTGAATCAAG CAAACCAAAG CAGTCCAGTG TTGAGTAGAC AGCAGTCCAT TACCCTACCC 
AACCAAGGCA GCCCGGTCCT AAGCAGACAG TCCTCGCTTA GTCATCCCAG TCAGGGTAGT 
CCTGTACTAG GACGCCACCC TTCCCTAACA CAGGGTAGCC CCAGCCTGGA TAGGCATCCG 
ATGTACAGCG GTTACACCAC TCCAGAAGAG CGACACGGAG CTCTGTCACG CCAAAGCAGC 
TCTTCAGGAT ACCAGCCGCC ATCTACACCG TCTTTCCCAG TTTCTCCAGC AGGATACATC 
GAGGGAGCGG GATTCAGACA GGGGAGTCCT GCTTTCCAAC CTCAGTTGCC CGAAAAGAGA 
CGCATGTCCA GCAGTGACCG CACCAATGGT GGCCTGTCCT ATGGGACGCT CAATGGGAAG 
ATGTCCTCAC CGGTGTCCAG TGGCGGCAGC ACTCCGAGTG TTCATTTCTT CCACACACTC 
CCCGATTTCT CCAAACTCAA CATGTGTGAT GGAAGTCCAG AAACCAGAAT GAACGTGAAG 
TTTGTCCAGG ACACGTCCAA ATTCTGGTAT AAACCAGATA TCTCGAGAGA CCAAGCGATT 
AACATGCTGA AGGATCAAGA TCCTGGGGCC TTTGTGATTC GGGACAGTCA TTCATTCAGA 
GGAGCCTACG GCTTGGCTAT GAAAGTGGCT TCACCACCTC CAACCACTCA ACCTACAAAG 
AAAGCTGGAG ATGTCACTAA TGAGTTGGTG AGGCATTTTC TAATTGAGAC GAGTCCGAAG 
GGTGTGAGAC TGAAGGGCTG CCCCAACGAG CCGTACTTTG GCTGCCTGTC TGCGCTGGTC 
TACCAGCACT CCATCACTCC ACTGGCACTG CCCTGCAAAC TAGTCATACC AACCAGAGAT 
CCTCTGGAAG AGTCACCTGA GATCGCAACA CCAACCAACC CGGCGGCAGA TATGCTCAAG 
CAGGGCGCAG GGCAGAAGAG TTCAGCTGAT GGCCATGCAT GCAATGTGCT GTACATCAAC 
TCTGTGGATA TGGAGTCTTT GACTGGACCT CAAGCCATCG CCAAAGCCAT TTCAGAAACC 
ATGAACGCCA GTCCGGCACC CTCAGCCACC ATTGTTCACT TCAAGGTGTC GGCACAAGGC 
ATCACACTCA CAGACAACCA GAGAAAGCTT TTCTTCAGAC GTCATTATCC CATCGGCACA 
GTCACGTTCT GTGACATTGA TCCACAGGAG AGGAAGTGGA ACAAACCAGA GGGTGGCACT 
GCCAAATTCT TTGGCTTTGT GGCTAGAAAG CAAGGCAGCA CGACAGACAA TGTCAGTCAC 
CTGTTTGCTG AGATGGACCC AGATCAGCCG GCCACCGCCA TCGTCAACTT TGTCTCTAAA 
GTGATGATCA GCTCTCAGAA GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334707.1
CDS	285..5711
Translation	MGCTVSFICCEDDFLQMPLDQHVEKKKEEKRLLFKPEEFEAVHSHTFRLKTFKKGKHCGVCKQTVNNEGLICRVCRLACHRKCEVKVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAEYTARIRCINDSQSAPEFGHRRRSLSFSGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSESEDEEEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAGQKSSADGHACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_021479032.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479032.1

ORF Insert Sequence:

ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA 
GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT 
TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT 
CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC 
CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG 
AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG 
AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT 
AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC 
AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG 
CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG 
ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG 
GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT 
TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT 
TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC 
AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC 
ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC 
TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG 
ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG 
TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC 
ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT 
AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA 
AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC 
CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC 
ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT 
AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC 
ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT 
GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG 
CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG 
GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT 
GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC 
AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC 
CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT 
CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA 
GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA 
GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG 
TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA 
ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA 
GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG 
TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG 
ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA 
GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC 
CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC 
TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT 
ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC 
ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA 
AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA 
TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA 
GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT 
CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG 
TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA 
GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG 
CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC 
TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT 
GGTCGATCTC CTCCAGGATT GGCTAAAACA CCACTGTCCT TTCTGGGCCT GAAGCCCCAC 
AACCCTGCTG AGATCCTGCT CAACCAGACA GGCTCAGAGA GTGAGGATGA GGAAGAGCCT 
CGTAGTTATG TGGAGTCTGT GGCGAAGGCA GCAGCAGTAC CTGCATCTCC AACATATGAC 
AGCGACAGCT TAGCTCGTCC TGCCGGACCG TCCCATGCTT ACAATGCTCC ACTTTCCTCC 
AGCAGCCCCA TTCAGAACCC AGATCCAGTT GAAAGCACAT TGGCTGACAG CGGGGTCGGG 
ATGAATTCTT TATCCCAGGC TGCTGTCGAC ATTTTCCATT TCCAGCCCTC CGAAATCCCA 
TACCAACCAC CACCACCCCC CTACCCAACC TCCATGTTGG GTAGCTACAT CAGCCCTTCT 
CCAGCAGAGG CCATGCAAAA TAATACCACC ACCATCCAGC CACTTTCTGG AAGTCCTGGG 
ACAAGACACC ACACCATCCT TTCTGACATG GCGTCCGTTC CCAACTTTCA ACACAGATTA 
GCAAATCAGG ATGGTCCCAT ACTTGGGCGA CAGCAGGCTC CAGCTAATGG TCATGTGCCT 
GGAATTGCTG GTAGTCCAGT GCAAGGAAGA CACACTATGG TGTCCCAGGG TACACAAAGC 
AGTCCCATAC TGAGCAGACA GTATCCTGTC ACCCAGGGGA TTCAGAGTAG TCCAATTCTC 
AGCAGGCAGC CAATGGGACA AGCTGTCCAG AGCAGTCCAG TCCTCAACCG ACAGGCATCC 
CTGAATCAAG CAAACCAAAG CAGTCCAGTG TTGAGTAGAC AGCAGTCCAT TACCCTACCC 
AACCAAGGCA GCCCGGTCCT AAGCAGACAG TCCTCGCTTA GTCATCCCAG TCAGGGTAGT 
CCTGTACTAG GACGCCACCC TTCCCTAACA CAGGGTAGCC CCAGCCTGGA TAGGCATCCG 
ATGTACAGCG GTTACACCAC TCCAGAAGAG CGACACGGAG CTCTGTCACG CCAAAGCAGC 
TCTTCAGGAT ACCAGCCGCC ATCTACACCG TCTTTCCCAG TTTCTCCAGC AGGATACATC 
GAGGGAGCGG GATTCAGACA GGGGAGTCCT GCTTTCCAAC CTCAGTTGCC CGAAAAGAGA 
CGCATGTCCA GCAGTGACCG CACCAATGGT GGCCTGTCCT ATGGGACGCT CAATGGGAAG 
ATGTCCTCAC CGGTGTCCAG TGGCGGCAGC ACTCCGAGTG TTCATTTCTT CCACACACTC 
CCCGATTTCT CCAAACTCAA CATGTGTGAT GGAAGTCCAG AAACCAGAAT GAACGTGAAG 
TTTGTCCAGG ACACGTCCAA ATTCTGGTAT AAACCAGATA TCTCGAGAGA CCAAGCGATT 
AACATGCTGA AGGATCAAGA TCCTGGGGCC TTTGTGATTC GGGACAGTCA TTCATTCAGA 
GGAGCCTACG GCTTGGCTAT GAAAGTGGCT TCACCACCTC CAACCACTCA ACCTACAAAG 
AAAGCTGGAG ATGTCACTAA TGAGTTGGTG AGGCATTTTC TAATTGAGAC GAGTCCGAAG 
GGTGTGAGAC TGAAGGGCTG CCCCAACGAG CCGTACTTTG GCTGCCTGTC TGCGCTGGTC 
TACCAGCACT CCATCACTCC ACTGGCACTG CCCTGCAAAC TAGTCATACC AACCAGAGAT 
CCTCTGGAAG AGTCACCTGA GATCGCAACA CCAACCAACC CGGCGGCAGA TATGCTCAAG 
CAGGGCGCAG GGCAGAAGAG TTCAGCTGAT GGCCATGCAT GCAATGTGCT GTACATCAAC 
TCTGTGGATA TGGAGTCTTT GACTGGACCT CAAGCCATCG CCAAAGCCAT TTCAGAAACC 
ATGAACGCCA GTCCGGCACC CTCAGCCACC ATTGTTCACT TCAAGGTGTC GGCACAAGGC 
ATCACACTCA CAGACAACCA GAGAAAGCTT TTCTTCAGAC GTCATTATCC CATCGGCACA 
GTCACGTTCT GTGACATTGA TCCACAGGAG AGGAAGTGGA ACAAACCAGA GGGTGGCACT 
GCCAAATTCT TTGGCTTTGT GGCTAGAAAG CAAGGCAGCA CGACAGACAA TGTCAGTCAC 
CTGTTTGCTG AGATGGACCC AGATCAGCCG GCCACCGCCA TCGTCAACTT TGTCTCTAAA 
GTGATGATCA GCTCTCAGAA GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_021479033.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334708.1 Danio rerio tensin 1b (tns1b), transcript variant X7, mRNA.

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CloneID	ODa144119
Clone ID Related Accession (Same CDS sequence)	XM_021479033.1
Accession Version	XM_021479033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA 
GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT 
TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT 
CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC 
CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG 
AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG 
AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT 
AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC 
AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG 
CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG 
ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG 
GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT 
TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT 
TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC 
AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC 
ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC 
TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG 
ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG 
TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC 
ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT 
AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA 
AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC 
CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC 
ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT 
AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC 
ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT 
GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG 
CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG 
GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT 
GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC 
AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC 
CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT 
CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA 
GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA 
GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG 
TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA 
ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA 
GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG 
TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG 
ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA 
GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC 
CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC 
TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT 
ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC 
ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA 
AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA 
TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA 
GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT 
CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG 
TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA 
GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG 
CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC 
TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT 
GGTCGATCTC CTCCAGGATT GGCTAAAACA CCACTGTCCT TTCTGGGCCT GAAGCCCCAC 
AACCCTGCTG AGATCCTGCT CAACCAGACA GGCTCAGAGC CTCGTAGTTA TGTGGAGTCT 
GTGGCGAAGG CAGCAGCAGT ACCTGCATCT CCAACATATG ACAGCGACAG CTTAGCTCGT 
CCTGCCGGAC CGTCCCATGC TTACAATGCT CCACTTTCCT CCAGCAGCCC CATTCAGAAC 
CCAGATCCAG TTGAAAGCAC ATTGGCTGAC AGCGGGGTCG GGATGAATTC TTTATCCCAG 
GCTGCTGTCG ACATTTTCCA TTTCCAGCCC TCCGAAATCC CATACCAACC ACCACCACCC 
CCCTACCCAA CCTCCATGTT GGGTAGCTAC ATCAGCCCTT CTCCAGCAGA GGCCATGCAA 
AATAATACCA CCACCATCCA GCCACTTTCT GGAAGTCCTG GGACAAGACA CCACACCATC 
CTTTCTGACA TGGCGTCCGT TCCCAACTTT CAACACAGAT TAGCAAATCA GGATGGTCCC 
ATACTTGGGC GACAGCAGGC TCCAGCTAAT GGTCATGTGC CTGGAATTGC TGGTAGTCCA 
GTGCAAGGAA GACACACTAT GGTGTCCCAG GGTACACAAA GCAGTCCCAT ACTGAGCAGA 
CAGTATCCTG TCACCCAGGG GATTCAGAGT AGTCCAATTC TCAGCAGGCA GCCAATGGGA 
CAAGCTGTCC AGAGCAGTCC AGTCCTCAAC CGACAGGCAT CCCTGAATCA AGCAAACCAA 
AGCAGTCCAG TGTTGAGTAG ACAGCAGTCC ATTACCCTAC CCAACCAAGG CAGCCCGGTC 
CTAAGCAGAC AGTCCTCGCT TAGTCATCCC AGTCAGGGTA GTCCTGTACT AGGACGCCAC 
CCTTCCCTAA CACAGGGTAG CCCCAGCCTG GATAGGCATC CGATGTACAG CGGTTACACC 
ACTCCAGAAG AGCGACACGG AGCTCTGTCA CGCCAAAGCA GCTCTTCAGG ATACCAGCCG 
CCATCTACAC CGTCTTTCCC AGTTTCTCCA GCAGGATACA TCGAGGGAGC GGGATTCAGA 
CAGGGGAGTC CTGCTTTCCA ACCTCAGTTG CCCGAAAAGA GACGCATGTC CAGCAGTGAC 
CGCACCAATG GTGGCCTGTC CTATGGGACG CTCAATGGGA AGATGTCCTC ACCGGTGTCC 
AGTGGCGGCA GCACTCCGAG TGTTCATTTC TTCCACACAC TCCCCGATTT CTCCAAACTC 
AACATGTGTG ATGGAAGTCC AGAAACCAGA ATGAACGTGA AGTTTGTCCA GGACACGTCC 
AAATTCTGGT ATAAACCAGA TATCTCGAGA GACCAAGCGA TTAACATGCT GAAGGATCAA 
GATCCTGGGG CCTTTGTGAT TCGGGACAGT CATTCATTCA GAGGAGCCTA CGGCTTGGCT 
ATGAAAGTGG CTTCACCACC TCCAACCACT CAACCTACAA AGAAAGCTGG AGATGTCACT 
AATGAGTTGG TGAGGCATTT TCTAATTGAG ACGAGTCCGA AGGGTGTGAG ACTGAAGGGC 
TGCCCCAACG AGCCGTACTT TGGCTGCCTG TCTGCGCTGG TCTACCAGCA CTCCATCACT 
CCACTGGCAC TGCCCTGCAA ACTAGTCATA CCAACCAGAG ATCCTCTGGA AGAGTCACCT 
GAGATCGCAA CACCAACCAA CCCGGCGGCA GATATGCTCA AGCAGGGCGC AGGGCAGAAG 
AGTTCAGCTG ATGGCCATGC ATGCAATGTG CTGTACATCA ACTCTGTGGA TATGGAGTCT 
TTGACTGGAC CTCAAGCCAT CGCCAAAGCC ATTTCAGAAA CCATGAACGC CAGTCCGGCA 
CCCTCAGCCA CCATTGTTCA CTTCAAGGTG TCGGCACAAG GCATCACACT CACAGACAAC 
CAGAGAAAGC TTTTCTTCAG ACGTCATTAT CCCATCGGCA CAGTCACGTT CTGTGACATT 
GATCCACAGG AGAGGAAGTG GAACAAACCA GAGGGTGGCA CTGCCAAATT CTTTGGCTTT 
GTGGCTAGAA AGCAAGGCAG CACGACAGAC AATGTCAGTC ACCTGTTTGC TGAGATGGAC 
CCAGATCAGC CGGCCACCGC CATCGTCAAC TTTGTCTCTA AAGTGATGAT CAGCTCTCAG 
AAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334708.1
CDS	284..5692
Translation	MGCTVSFICCEDDFLQMPLDQHVEKKKEEKRLLFKPEEFEAVHSHTFRLKTFKKGKHCGVCKQTVNNEGLICRVCRLACHRKCEVKVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAEYTARIRCINDSQSAPEFGHRRRSLSFSGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAGQKSSADGHACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_021479033.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479033.1

ORF Insert Sequence:

ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA 
GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT 
TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT 
CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC 
CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG 
AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG 
AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT 
AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC 
AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG 
CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG 
ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG 
GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT 
TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT 
TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC 
AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC 
ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC 
TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG 
ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG 
TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC 
ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT 
AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA 
AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC 
CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC 
ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT 
AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC 
ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT 
GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG 
CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG 
GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT 
GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC 
AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC 
CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT 
CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA 
GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA 
GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG 
TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA 
ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA 
GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG 
TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG 
ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA 
GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC 
CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC 
TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT 
ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC 
ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA 
AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA 
TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA 
GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT 
CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG 
TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA 
GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG 
CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC 
TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT 
GGTCGATCTC CTCCAGGATT GGCTAAAACA CCACTGTCCT TTCTGGGCCT GAAGCCCCAC 
AACCCTGCTG AGATCCTGCT CAACCAGACA GGCTCAGAGC CTCGTAGTTA TGTGGAGTCT 
GTGGCGAAGG CAGCAGCAGT ACCTGCATCT CCAACATATG ACAGCGACAG CTTAGCTCGT 
CCTGCCGGAC CGTCCCATGC TTACAATGCT CCACTTTCCT CCAGCAGCCC CATTCAGAAC 
CCAGATCCAG TTGAAAGCAC ATTGGCTGAC AGCGGGGTCG GGATGAATTC TTTATCCCAG 
GCTGCTGTCG ACATTTTCCA TTTCCAGCCC TCCGAAATCC CATACCAACC ACCACCACCC 
CCCTACCCAA CCTCCATGTT GGGTAGCTAC ATCAGCCCTT CTCCAGCAGA GGCCATGCAA 
AATAATACCA CCACCATCCA GCCACTTTCT GGAAGTCCTG GGACAAGACA CCACACCATC 
CTTTCTGACA TGGCGTCCGT TCCCAACTTT CAACACAGAT TAGCAAATCA GGATGGTCCC 
ATACTTGGGC GACAGCAGGC TCCAGCTAAT GGTCATGTGC CTGGAATTGC TGGTAGTCCA 
GTGCAAGGAA GACACACTAT GGTGTCCCAG GGTACACAAA GCAGTCCCAT ACTGAGCAGA 
CAGTATCCTG TCACCCAGGG GATTCAGAGT AGTCCAATTC TCAGCAGGCA GCCAATGGGA 
CAAGCTGTCC AGAGCAGTCC AGTCCTCAAC CGACAGGCAT CCCTGAATCA AGCAAACCAA 
AGCAGTCCAG TGTTGAGTAG ACAGCAGTCC ATTACCCTAC CCAACCAAGG CAGCCCGGTC 
CTAAGCAGAC AGTCCTCGCT TAGTCATCCC AGTCAGGGTA GTCCTGTACT AGGACGCCAC 
CCTTCCCTAA CACAGGGTAG CCCCAGCCTG GATAGGCATC CGATGTACAG CGGTTACACC 
ACTCCAGAAG AGCGACACGG AGCTCTGTCA CGCCAAAGCA GCTCTTCAGG ATACCAGCCG 
CCATCTACAC CGTCTTTCCC AGTTTCTCCA GCAGGATACA TCGAGGGAGC GGGATTCAGA 
CAGGGGAGTC CTGCTTTCCA ACCTCAGTTG CCCGAAAAGA GACGCATGTC CAGCAGTGAC 
CGCACCAATG GTGGCCTGTC CTATGGGACG CTCAATGGGA AGATGTCCTC ACCGGTGTCC 
AGTGGCGGCA GCACTCCGAG TGTTCATTTC TTCCACACAC TCCCCGATTT CTCCAAACTC 
AACATGTGTG ATGGAAGTCC AGAAACCAGA ATGAACGTGA AGTTTGTCCA GGACACGTCC 
AAATTCTGGT ATAAACCAGA TATCTCGAGA GACCAAGCGA TTAACATGCT GAAGGATCAA 
GATCCTGGGG CCTTTGTGAT TCGGGACAGT CATTCATTCA GAGGAGCCTA CGGCTTGGCT 
ATGAAAGTGG CTTCACCACC TCCAACCACT CAACCTACAA AGAAAGCTGG AGATGTCACT 
AATGAGTTGG TGAGGCATTT TCTAATTGAG ACGAGTCCGA AGGGTGTGAG ACTGAAGGGC 
TGCCCCAACG AGCCGTACTT TGGCTGCCTG TCTGCGCTGG TCTACCAGCA CTCCATCACT 
CCACTGGCAC TGCCCTGCAA ACTAGTCATA CCAACCAGAG ATCCTCTGGA AGAGTCACCT 
GAGATCGCAA CACCAACCAA CCCGGCGGCA GATATGCTCA AGCAGGGCGC AGGGCAGAAG 
AGTTCAGCTG ATGGCCATGC ATGCAATGTG CTGTACATCA ACTCTGTGGA TATGGAGTCT 
TTGACTGGAC CTCAAGCCAT CGCCAAAGCC ATTTCAGAAA CCATGAACGC CAGTCCGGCA 
CCCTCAGCCA CCATTGTTCA CTTCAAGGTG TCGGCACAAG GCATCACACT CACAGACAAC 
CAGAGAAAGC TTTTCTTCAG ACGTCATTAT CCCATCGGCA CAGTCACGTT CTGTGACATT 
GATCCACAGG AGAGGAAGTG GAACAAACCA GAGGGTGGCA CTGCCAAATT CTTTGGCTTT 
GTGGCTAGAA AGCAAGGCAG CACGACAGAC AATGTCAGTC ACCTGTTTGC TGAGATGGAC 
CCAGATCAGC CGGCCACCGC CATCGTCAAC TTTGTCTCTA AAGTGATGAT CAGCTCTCAG 
AAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_021479034.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334709.1 Danio rerio tensin 1b (tns1b), transcript variant X8, mRNA.

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CloneID	ODa144120
Clone ID Related Accession (Same CDS sequence)	XM_021479034.1
Accession Version	XM_021479034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA 
GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT 
TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT 
CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC 
CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG 
AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG 
AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT 
AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC 
AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG 
CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG 
ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG 
GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT 
TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT 
TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC 
AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC 
ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC 
TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG 
ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG 
TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC 
ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT 
AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA 
AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC 
CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC 
ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT 
AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC 
ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT 
GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG 
CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG 
GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT 
GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC 
AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC 
CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT 
CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA 
GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA 
GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG 
TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA 
ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA 
GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG 
TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG 
ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA 
GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC 
CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC 
TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT 
ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC 
ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA 
AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA 
TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA 
GCTCACCGTG TAGCTGGACT TCCATCTGGT GAAATGTCGC CAGATGAGGC TGCAGTTCCT 
GGACGCTGTC GGACCCTTAG TGAGGGCCTT TCCCAAGACG AAAGCCCAAC TCGAGGAAGC 
TCAGTCCGCT CCCCCATTCG CTGCATCTCA CCAGAGCTGG CCAATACAAT TGCTCTGAAC 
CCTGGGGGGC GGCCCAAGGA GAGGCACATG CACAGCTACA GAGAAGCGTT TGAGGAAATG 
GATGGGGGTC CCGTTAGCCC CCCTCCAATT GTTGGTGGTG AGGTCCTACC CCAAACCCCT 
GCCTTCCCTG TCTCGCCACA AACCCCTTAC TTCAACATGT GTCGATCTCC TCCAGGATTG 
GCTAAAACAC CACTGTCCTT TCTGGGCCTG AAGCCCCACA ACCCTGCTGA GATCCTGCTC 
AACCAGACAG GCTCAGAGAG TGAGGATGAG GAAGAGCCTC GTAGTTATGT GGAGTCTGTG 
GCGAAGGCAG CAGCAGTACC TGCATCTCCA ACATATGACA GCGACAGCTT AGCTCGTCCT 
GCCGGACCGT CCCATGCTTA CAATGCTCCA CTTTCCTCCA GCAGCCCCAT TCAGAACCCA 
GATCCAGTTG AAAGCACATT GGCTGACAGC GGGGTCGGGA TGAATTCTTT ATCCCAGGCT 
GCTGTCGACA TTTTCCATTT CCAGCCCTCC GAAATCCCAT ACCAACCACC ACCACCCCCC 
TACCCAACCT CCATGTTGGG TAGCTACATC AGCCCTTCTC CAGCAGAGGC CATGCAAAAT 
AATACCACCA CCATCCAGCC ACTTTCTGGA AGTCCTGGGA CAAGACACCA CACCATCCTT 
TCTGACATGG CGTCCGTTCC CAACTTTCAA CACAGATTAG CAAATCAGGA TGGTCCCATA 
CTTGGGCGAC AGCAGGCTCC AGCTAATGGT CATGTGCCTG GAATTGCTGG TAGTCCAGTG 
CAAGGAAGAC ACACTATGGT GTCCCAGGGT ACACAAAGCA GTCCCATACT GAGCAGACAG 
TATCCTGTCA CCCAGGGGAT TCAGAGTAGT CCAATTCTCA GCAGGCAGCC AATGGGACAA 
GCTGTCCAGA GCAGTCCAGT CCTCAACCGA CAGGCATCCC TGAATCAAGC AAACCAAAGC 
AGTCCAGTGT TGAGTAGACA GCAGTCCATT ACCCTACCCA ACCAAGGCAG CCCGGTCCTA 
AGCAGACAGT CCTCGCTTAG TCATCCCAGT CAGGGTAGTC CTGTACTAGG ACGCCACCCT 
TCCCTAACAC AGGGTAGCCC CAGCCTGGAT AGGCATCCGA TGTACAGCGG TTACACCACT 
CCAGAAGAGC GACACGGAGC TCTGTCACGC CAAAGCAGCT CTTCAGGATA CCAGCCGCCA 
TCTACACCGT CTTTCCCAGT TTCTCCAGCA GGATACATCG AGGGAGCGGG ATTCAGACAG 
GGGAGTCCTG CTTTCCAACC TCAGTTGCCC GAAAAGAGAC GCATGTCCAG CAGTGACCGC 
ACCAATGGTG GCCTGTCCTA TGGGACGCTC AATGGGAAGA TGTCCTCACC GGTGTCCAGT 
GGCGGCAGCA CTCCGAGTGT TCATTTCTTC CACACACTCC CCGATTTCTC CAAACTCAAC 
ATGTGTGATG GAAGTCCAGA AACCAGAATG AACGTGAAGT TTGTCCAGGA CACGTCCAAA 
TTCTGGTATA AACCAGATAT CTCGAGAGAC CAAGCGATTA ACATGCTGAA GGATCAAGAT 
CCTGGGGCCT TTGTGATTCG GGACAGTCAT TCATTCAGAG GAGCCTACGG CTTGGCTATG 
AAAGTGGCTT CACCACCTCC AACCACTCAA CCTACAAAGA AAGCTGGAGA TGTCACTAAT 
GAGTTGGTGA GGCATTTTCT AATTGAGACG AGTCCGAAGG GTGTGAGACT GAAGGGCTGC 
CCCAACGAGC CGTACTTTGG CTGCCTGTCT GCGCTGGTCT ACCAGCACTC CATCACTCCA 
CTGGCACTGC CCTGCAAACT AGTCATACCA ACCAGAGATC CTCTGGAAGA GTCACCTGAG 
ATCGCAACAC CAACCAACCC GGCGGCAGAT ATGCTCAAGC AGGGCGCAGG GCAGAAGAGT 
TCAGCTGATG GCCATGCATG CAATGTGCTG TACATCAACT CTGTGGATAT GGAGTCTTTG 
ACTGGACCTC AAGCCATCGC CAAAGCCATT TCAGAAACCA TGAACGCCAG TCCGGCACCC 
TCAGCCACCA TTGTTCACTT CAAGGTGTCG GCACAAGGCA TCACACTCAC AGACAACCAG 
AGAAAGCTTT TCTTCAGACG TCATTATCCC ATCGGCACAG TCACGTTCTG TGACATTGAT 
CCACAGGAGA GGAAGTGGAA CAAACCAGAG GGTGGCACTG CCAAATTCTT TGGCTTTGTG 
GCTAGAAAGC AAGGCAGCAC GACAGACAAT GTCAGTCACC TGTTTGCTGA GATGGACCCA 
GATCAGCCGG CCACCGCCAT CGTCAACTTT GTCTCTAAAG TGATGATCAG CTCTCAGAAG 
CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334709.1
CDS	284..5689
Translation	MGCTVSFICCEDDFLQMPLDQHVEKKKEEKRLLFKPEEFEAVHSHTFRLKTFKKGKHCGVCKQTVNNEGLICRVCRLACHRKCEVKVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGEVLPQTPAFPVSPQTPYFNMCRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSESEDEEEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAGQKSSADGHACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_021479034.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479034.1

ORF Insert Sequence:

ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA 
GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT 
TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT 
CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC 
CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG 
AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG 
AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT 
AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC 
AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG 
CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG 
ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG 
GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT 
TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT 
TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC 
AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC 
ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC 
TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG 
ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG 
TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC 
ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT 
AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA 
AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC 
CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC 
ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT 
AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC 
ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT 
GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG 
CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG 
GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT 
GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC 
AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC 
CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT 
CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA 
GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA 
GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG 
TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA 
ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA 
GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG 
TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG 
ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA 
GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC 
CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC 
TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT 
ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC 
ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA 
AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA 
TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA 
GCTCACCGTG TAGCTGGACT TCCATCTGGT GAAATGTCGC CAGATGAGGC TGCAGTTCCT 
GGACGCTGTC GGACCCTTAG TGAGGGCCTT TCCCAAGACG AAAGCCCAAC TCGAGGAAGC 
TCAGTCCGCT CCCCCATTCG CTGCATCTCA CCAGAGCTGG CCAATACAAT TGCTCTGAAC 
CCTGGGGGGC GGCCCAAGGA GAGGCACATG CACAGCTACA GAGAAGCGTT TGAGGAAATG 
GATGGGGGTC CCGTTAGCCC CCCTCCAATT GTTGGTGGTG AGGTCCTACC CCAAACCCCT 
GCCTTCCCTG TCTCGCCACA AACCCCTTAC TTCAACATGT GTCGATCTCC TCCAGGATTG 
GCTAAAACAC CACTGTCCTT TCTGGGCCTG AAGCCCCACA ACCCTGCTGA GATCCTGCTC 
AACCAGACAG GCTCAGAGAG TGAGGATGAG GAAGAGCCTC GTAGTTATGT GGAGTCTGTG 
GCGAAGGCAG CAGCAGTACC TGCATCTCCA ACATATGACA GCGACAGCTT AGCTCGTCCT 
GCCGGACCGT CCCATGCTTA CAATGCTCCA CTTTCCTCCA GCAGCCCCAT TCAGAACCCA 
GATCCAGTTG AAAGCACATT GGCTGACAGC GGGGTCGGGA TGAATTCTTT ATCCCAGGCT 
GCTGTCGACA TTTTCCATTT CCAGCCCTCC GAAATCCCAT ACCAACCACC ACCACCCCCC 
TACCCAACCT CCATGTTGGG TAGCTACATC AGCCCTTCTC CAGCAGAGGC CATGCAAAAT 
AATACCACCA CCATCCAGCC ACTTTCTGGA AGTCCTGGGA CAAGACACCA CACCATCCTT 
TCTGACATGG CGTCCGTTCC CAACTTTCAA CACAGATTAG CAAATCAGGA TGGTCCCATA 
CTTGGGCGAC AGCAGGCTCC AGCTAATGGT CATGTGCCTG GAATTGCTGG TAGTCCAGTG 
CAAGGAAGAC ACACTATGGT GTCCCAGGGT ACACAAAGCA GTCCCATACT GAGCAGACAG 
TATCCTGTCA CCCAGGGGAT TCAGAGTAGT CCAATTCTCA GCAGGCAGCC AATGGGACAA 
GCTGTCCAGA GCAGTCCAGT CCTCAACCGA CAGGCATCCC TGAATCAAGC AAACCAAAGC 
AGTCCAGTGT TGAGTAGACA GCAGTCCATT ACCCTACCCA ACCAAGGCAG CCCGGTCCTA 
AGCAGACAGT CCTCGCTTAG TCATCCCAGT CAGGGTAGTC CTGTACTAGG ACGCCACCCT 
TCCCTAACAC AGGGTAGCCC CAGCCTGGAT AGGCATCCGA TGTACAGCGG TTACACCACT 
CCAGAAGAGC GACACGGAGC TCTGTCACGC CAAAGCAGCT CTTCAGGATA CCAGCCGCCA 
TCTACACCGT CTTTCCCAGT TTCTCCAGCA GGATACATCG AGGGAGCGGG ATTCAGACAG 
GGGAGTCCTG CTTTCCAACC TCAGTTGCCC GAAAAGAGAC GCATGTCCAG CAGTGACCGC 
ACCAATGGTG GCCTGTCCTA TGGGACGCTC AATGGGAAGA TGTCCTCACC GGTGTCCAGT 
GGCGGCAGCA CTCCGAGTGT TCATTTCTTC CACACACTCC CCGATTTCTC CAAACTCAAC 
ATGTGTGATG GAAGTCCAGA AACCAGAATG AACGTGAAGT TTGTCCAGGA CACGTCCAAA 
TTCTGGTATA AACCAGATAT CTCGAGAGAC CAAGCGATTA ACATGCTGAA GGATCAAGAT 
CCTGGGGCCT TTGTGATTCG GGACAGTCAT TCATTCAGAG GAGCCTACGG CTTGGCTATG 
AAAGTGGCTT CACCACCTCC AACCACTCAA CCTACAAAGA AAGCTGGAGA TGTCACTAAT 
GAGTTGGTGA GGCATTTTCT AATTGAGACG AGTCCGAAGG GTGTGAGACT GAAGGGCTGC 
CCCAACGAGC CGTACTTTGG CTGCCTGTCT GCGCTGGTCT ACCAGCACTC CATCACTCCA 
CTGGCACTGC CCTGCAAACT AGTCATACCA ACCAGAGATC CTCTGGAAGA GTCACCTGAG 
ATCGCAACAC CAACCAACCC GGCGGCAGAT ATGCTCAAGC AGGGCGCAGG GCAGAAGAGT 
TCAGCTGATG GCCATGCATG CAATGTGCTG TACATCAACT CTGTGGATAT GGAGTCTTTG 
ACTGGACCTC AAGCCATCGC CAAAGCCATT TCAGAAACCA TGAACGCCAG TCCGGCACCC 
TCAGCCACCA TTGTTCACTT CAAGGTGTCG GCACAAGGCA TCACACTCAC AGACAACCAG 
AGAAAGCTTT TCTTCAGACG TCATTATCCC ATCGGCACAG TCACGTTCTG TGACATTGAT 
CCACAGGAGA GGAAGTGGAA CAAACCAGAG GGTGGCACTG CCAAATTCTT TGGCTTTGTG 
GCTAGAAAGC AAGGCAGCAC GACAGACAAT GTCAGTCACC TGTTTGCTGA GATGGACCCA 
GATCAGCCGG CCACCGCCAT CGTCAACTTT GTCTCTAAAG TGATGATCAG CTCTCAGAAG 
CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_021479035.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334710.1 Danio rerio tensin 1b (tns1b), transcript variant X9, mRNA.

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CloneID	ODa144121
Clone ID Related Accession (Same CDS sequence)	XM_021479035.1
Accession Version	XM_021479035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA 
GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT 
TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT 
CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC 
CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG 
AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG 
AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT 
AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC 
AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG 
CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG 
ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG 
GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT 
TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT 
TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC 
AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC 
ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC 
TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG 
ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG 
TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC 
ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT 
AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA 
AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC 
CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC 
ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT 
AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC 
ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT 
GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG 
CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG 
GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT 
GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC 
AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC 
CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT 
CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA 
GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA 
GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG 
TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA 
ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA 
GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG 
TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG 
ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA 
GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC 
CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC 
TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT 
ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC 
ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA 
AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA 
TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA 
GCTCACCGTG TAGCTGGACT TCCATCTGGT GAAATGTCGC CAGATGAGGC TGCAGTTCCT 
GGACGCTGTC GGACCCTTAG TGAGGGCCTT TCCCAAGACG AAAGCCCAAC TCGAGGAAGC 
TCAGTCCGCT CCCCCATTCG CTGCATCTCA CCAGAGCTGG CCAATACAAT TGCTCTGAAC 
CCTGGGGGGC GGCCCAAGGA GAGGCACATG CACAGCTACA GAGAAGCGTT TGAGGAAATG 
GATGGGGGTC CCGTTAGCCC CCCTCCAATT GTTGGTGGTC GATCTCCTCC AGGATTGGCT 
AAAACACCAC TGTCCTTTCT GGGCCTGAAG CCCCACAACC CTGCTGAGAT CCTGCTCAAC 
CAGACAGGCT CAGAGCCTCG TAGTTATGTG GAGTCTGTGG CGAAGGCAGC AGCAGTACCT 
GCATCTCCAA CATATGACAG CGACAGCTTA GCTCGTCCTG CCGGACCGTC CCATGCTTAC 
AATGCTCCAC TTTCCTCCAG CAGCCCCATT CAGAACCCAG ATCCAGTTGA AAGCACATTG 
GCTGACAGCG GGGTCGGGAT GAATTCTTTA TCCCAGGCTG CTGTCGACAT TTTCCATTTC 
CAGCCCTCCG AAATCCCATA CCAACCACCA CCACCCCCCT ACCCAACCTC CATGTTGGGT 
AGCTACATCA GCCCTTCTCC AGCAGAGGCC ATGCAAAATA ATACCACCAC CATCCAGCCA 
CTTTCTGGAA GTCCTGGGAC AAGACACCAC ACCATCCTTT CTGACATGGC GTCCGTTCCC 
AACTTTCAAC ACAGATTAGC AAATCAGGAT GGTCCCATAC TTGGGCGACA GCAGGCTCCA 
GCTAATGGTC ATGTGCCTGG AATTGCTGGT AGTCCAGTGC AAGGAAGACA CACTATGGTG 
TCCCAGGGTA CACAAAGCAG TCCCATACTG AGCAGACAGT ATCCTGTCAC CCAGGGGATT 
CAGAGTAGTC CAATTCTCAG CAGGCAGCCA ATGGGACAAG CTGTCCAGAG CAGTCCAGTC 
CTCAACCGAC AGGCATCCCT GAATCAAGCA AACCAAAGCA GTCCAGTGTT GAGTAGACAG 
CAGTCCATTA CCCTACCCAA CCAAGGCAGC CCGGTCCTAA GCAGACAGTC CTCGCTTAGT 
CATCCCAGTC AGGGTAGTCC TGTACTAGGA CGCCACCCTT CCCTAACACA GGGTAGCCCC 
AGCCTGGATA GGCATCCGAT GTACAGCGGT TACACCACTC CAGAAGAGCG ACACGGAGCT 
CTGTCACGCC AAAGCAGCTC TTCAGGATAC CAGCCGCCAT CTACACCGTC TTTCCCAGTT 
TCTCCAGCAG GATACATCGA GGGAGCGGGA TTCAGACAGG GGAGTCCTGC TTTCCAACCT 
CAGTTGCCCG AAAAGAGACG CATGTCCAGC AGTGACCGCA CCAATGGTGG CCTGTCCTAT 
GGGACGCTCA ATGGGAAGAT GTCCTCACCG GTGTCCAGTG GCGGCAGCAC TCCGAGTGTT 
CATTTCTTCC ACACACTCCC CGATTTCTCC AAACTCAACA TGTGTGATGG AAGTCCAGAA 
ACCAGAATGA ACGTGAAGTT TGTCCAGGAC ACGTCCAAAT TCTGGTATAA ACCAGATATC 
TCGAGAGACC AAGCGATTAA CATGCTGAAG GATCAAGATC CTGGGGCCTT TGTGATTCGG 
GACAGTCATT CATTCAGAGG AGCCTACGGC TTGGCTATGA AAGTGGCTTC ACCACCTCCA 
ACCACTCAAC CTACAAAGAA AGCTGGAGAT GTCACTAATG AGTTGGTGAG GCATTTTCTA 
ATTGAGACGA GTCCGAAGGG TGTGAGACTG AAGGGCTGCC CCAACGAGCC GTACTTTGGC 
TGCCTGTCTG CGCTGGTCTA CCAGCACTCC ATCACTCCAC TGGCACTGCC CTGCAAACTA 
GTCATACCAA CCAGAGATCC TCTGGAAGAG TCACCTGAGA TCGCAACACC AACCAACCCG 
GCGGCAGATA TGCTCAAGCA GGGCGCAGGG CAGAAGAGTT CAGCTGATGG CCATGCATGC 
AATGTGCTGT ACATCAACTC TGTGGATATG GAGTCTTTGA CTGGACCTCA AGCCATCGCC 
AAAGCCATTT CAGAAACCAT GAACGCCAGT CCGGCACCCT CAGCCACCAT TGTTCACTTC 
AAGGTGTCGG CACAAGGCAT CACACTCACA GACAACCAGA GAAAGCTTTT CTTCAGACGT 
CATTATCCCA TCGGCACAGT CACGTTCTGT GACATTGATC CACAGGAGAG GAAGTGGAAC 
AAACCAGAGG GTGGCACTGC CAAATTCTTT GGCTTTGTGG CTAGAAAGCA AGGCAGCACG 
ACAGACAATG TCAGTCACCT GTTTGCTGAG ATGGACCCAG ATCAGCCGGC CACCGCCATC 
GTCAACTTTG TCTCTAAAGT GATGATCAGC TCTCAGAAGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334710.1
CDS	283..5607
Translation	MGCTVSFICCEDDFLQMPLDQHVEKKKEEKRLLFKPEEFEAVHSHTFRLKTFKKGKHCGVCKQTVNNEGLICRVCRLACHRKCEVKVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAGQKSSADGHACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_021479035.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479035.1

ORF Insert Sequence:

ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA 
GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT 
TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT 
CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC 
CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG 
AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG 
AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT 
AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC 
AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG 
CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG 
ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG 
GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT 
TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT 
TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC 
AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC 
ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC 
TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG 
ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG 
TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC 
ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT 
AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA 
AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC 
CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC 
ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT 
AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC 
ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT 
GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG 
CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG 
GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT 
GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC 
AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC 
CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT 
CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA 
GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA 
GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG 
TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA 
ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA 
GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG 
TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG 
ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA 
GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC 
CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC 
TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT 
ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC 
ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA 
AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA 
TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA 
GCTCACCGTG TAGCTGGACT TCCATCTGGT GAAATGTCGC CAGATGAGGC TGCAGTTCCT 
GGACGCTGTC GGACCCTTAG TGAGGGCCTT TCCCAAGACG AAAGCCCAAC TCGAGGAAGC 
TCAGTCCGCT CCCCCATTCG CTGCATCTCA CCAGAGCTGG CCAATACAAT TGCTCTGAAC 
CCTGGGGGGC GGCCCAAGGA GAGGCACATG CACAGCTACA GAGAAGCGTT TGAGGAAATG 
GATGGGGGTC CCGTTAGCCC CCCTCCAATT GTTGGTGGTC GATCTCCTCC AGGATTGGCT 
AAAACACCAC TGTCCTTTCT GGGCCTGAAG CCCCACAACC CTGCTGAGAT CCTGCTCAAC 
CAGACAGGCT CAGAGCCTCG TAGTTATGTG GAGTCTGTGG CGAAGGCAGC AGCAGTACCT 
GCATCTCCAA CATATGACAG CGACAGCTTA GCTCGTCCTG CCGGACCGTC CCATGCTTAC 
AATGCTCCAC TTTCCTCCAG CAGCCCCATT CAGAACCCAG ATCCAGTTGA AAGCACATTG 
GCTGACAGCG GGGTCGGGAT GAATTCTTTA TCCCAGGCTG CTGTCGACAT TTTCCATTTC 
CAGCCCTCCG AAATCCCATA CCAACCACCA CCACCCCCCT ACCCAACCTC CATGTTGGGT 
AGCTACATCA GCCCTTCTCC AGCAGAGGCC ATGCAAAATA ATACCACCAC CATCCAGCCA 
CTTTCTGGAA GTCCTGGGAC AAGACACCAC ACCATCCTTT CTGACATGGC GTCCGTTCCC 
AACTTTCAAC ACAGATTAGC AAATCAGGAT GGTCCCATAC TTGGGCGACA GCAGGCTCCA 
GCTAATGGTC ATGTGCCTGG AATTGCTGGT AGTCCAGTGC AAGGAAGACA CACTATGGTG 
TCCCAGGGTA CACAAAGCAG TCCCATACTG AGCAGACAGT ATCCTGTCAC CCAGGGGATT 
CAGAGTAGTC CAATTCTCAG CAGGCAGCCA ATGGGACAAG CTGTCCAGAG CAGTCCAGTC 
CTCAACCGAC AGGCATCCCT GAATCAAGCA AACCAAAGCA GTCCAGTGTT GAGTAGACAG 
CAGTCCATTA CCCTACCCAA CCAAGGCAGC CCGGTCCTAA GCAGACAGTC CTCGCTTAGT 
CATCCCAGTC AGGGTAGTCC TGTACTAGGA CGCCACCCTT CCCTAACACA GGGTAGCCCC 
AGCCTGGATA GGCATCCGAT GTACAGCGGT TACACCACTC CAGAAGAGCG ACACGGAGCT 
CTGTCACGCC AAAGCAGCTC TTCAGGATAC CAGCCGCCAT CTACACCGTC TTTCCCAGTT 
TCTCCAGCAG GATACATCGA GGGAGCGGGA TTCAGACAGG GGAGTCCTGC TTTCCAACCT 
CAGTTGCCCG AAAAGAGACG CATGTCCAGC AGTGACCGCA CCAATGGTGG CCTGTCCTAT 
GGGACGCTCA ATGGGAAGAT GTCCTCACCG GTGTCCAGTG GCGGCAGCAC TCCGAGTGTT 
CATTTCTTCC ACACACTCCC CGATTTCTCC AAACTCAACA TGTGTGATGG AAGTCCAGAA 
ACCAGAATGA ACGTGAAGTT TGTCCAGGAC ACGTCCAAAT TCTGGTATAA ACCAGATATC 
TCGAGAGACC AAGCGATTAA CATGCTGAAG GATCAAGATC CTGGGGCCTT TGTGATTCGG 
GACAGTCATT CATTCAGAGG AGCCTACGGC TTGGCTATGA AAGTGGCTTC ACCACCTCCA 
ACCACTCAAC CTACAAAGAA AGCTGGAGAT GTCACTAATG AGTTGGTGAG GCATTTTCTA 
ATTGAGACGA GTCCGAAGGG TGTGAGACTG AAGGGCTGCC CCAACGAGCC GTACTTTGGC 
TGCCTGTCTG CGCTGGTCTA CCAGCACTCC ATCACTCCAC TGGCACTGCC CTGCAAACTA 
GTCATACCAA CCAGAGATCC TCTGGAAGAG TCACCTGAGA TCGCAACACC AACCAACCCG 
GCGGCAGATA TGCTCAAGCA GGGCGCAGGG CAGAAGAGTT CAGCTGATGG CCATGCATGC 
AATGTGCTGT ACATCAACTC TGTGGATATG GAGTCTTTGA CTGGACCTCA AGCCATCGCC 
AAAGCCATTT CAGAAACCAT GAACGCCAGT CCGGCACCCT CAGCCACCAT TGTTCACTTC 
AAGGTGTCGG CACAAGGCAT CACACTCACA GACAACCAGA GAAAGCTTTT CTTCAGACGT 
CATTATCCCA TCGGCACAGT CACGTTCTGT GACATTGATC CACAGGAGAG GAAGTGGAAC 
AAACCAGAGG GTGGCACTGC CAAATTCTTT GGCTTTGTGG CTAGAAAGCA AGGCAGCACG 
ACAGACAATG TCAGTCACCT GTTTGCTGAG ATGGACCCAG ATCAGCCGGC CACCGCCATC 
GTCAACTTTG TCTCTAAAGT GATGATCAGC TCTCAGAAGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_021479036.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334711.1 Danio rerio tensin 1b (tns1b), transcript variant X10, mRNA.

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CloneID	ODa144122
Clone ID Related Accession (Same CDS sequence)	XM_021479036.1
Accession Version	XM_021479036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCG CTGTGTTTCA GGTCTCGTCC TCCTGTGTTC CTGTCGCCAA CTATGAACTG 
GCCCCCAGCA ATGAGCTCCC CCTGAAACAT ATCGAGACCA CAGGCTCAAC CAAATCCTCC 
AAGAGCATGG AGTCTCGACG CAGACCGTCA AGGAGTTTGA GTTTGATCCA GGCCATGGAG 
GAGAACTATG AGGTGGATCT GGTTTACATC ACCGAGCGCA TCATTTCTGT CACCTTTCCC 
AGTAATGCAG AGGAGCCCAG TTACAGCGCC AACATTAAAG AAGTGGCAAA CATGCTGCGC 
TCCAAACATG GAGACAACTA CCTGCTCTTC AACCTCAGTG AAAAGCGCTT GGATATCTCC 
AAGCTGAATC CCAAAGTTCT GGATTTTGGC TGGCCGGATC ATCACGCTCC TGCTTTGGAT 
AAGATCTGCA GCATCTGCAA AGCCATGGAC ACTTGGATGA ACGCTGGCAG TCACAACGTG 
GTGGTCATAC ACAACAAGGG AAACCGAGGT CGAACTGGGG TGGTTGTGGC TGCGTACATG 
CATTACAGCA ACATATCAGC GAGTGCCGAC CAGGCTCTGG ACAGGTTTGC CATGAAGAGG 
TTTTATGAAG ATAAAGTGCT TCCTGTGGGT CAGCCTTCCC AGAGAAGATA TGTGCAGTAC 
TTCAGCGGTC TGCTCTCCGG CCACATTAAG ATCAACAACA AACCCCTGTT TCTCCATCAC 
GTCATCATGC ACGGCATCCC AAACTTCGAG TCTAAAGGAG GTTGCCGCCC TTTCCTGAAG 
ATCTACCAGG CCATGCAGCC CGTCTACACA TCTGGCATCT ATAATGTCCA AGGAGACAGT 
CAGACTAGTA TCTGCATCAC CATTGAACCG GGCCTGCTTT TGAAAGGAGA CATACTGCTA 
AAGTGTTATC ACAAGCGTTT CCGCAGCCCA TGCCGTGATG TCATATTCAG AGTTCAGTTT 
CACACCTGTG CTGTTCATGA TCTGGGTATC GTCTTCGGCA AAGATGAGCT TGATGAAACC 
TTTAAAGACG ACAGATTTCC AGAATATGGA AAGGTGGAAT TCGTCTTTTC TTTTGGCCCA 
GAAAAAATCC AAGGAATGGA CCACCTTGAG AACGGGCCCA GTGTTTCTGT GGACTACAAT 
ACCCAAGATG CACTAATCCG CTGGGATTCC TACGAGAACT TCAACCAGCG CTGTGAAGAC 
TCCATGGAAG ACGTTATTCA CACCGAAGGT CCATTGGATG GTAGTCTCTA CGCTAAAGTT 
CGTAAGAAAG AGTCTCTGGA AGGAGGGGTT ACAGCCAATG GTCTCCCAGC TGCTGCTGAT 
CACACTTTAC CTGCTGTAGA CCACACTCTA CCACCAGTTG ACCACGCCTT GTCTGTAAGC 
AGTGATTCAG GCAACTCCAC AGCGTCCATC AAGACTGACC GCACTGATGA AGCCAGCCAT 
CAGCCAGCAT TGGTGAGTCA GCCATCTTCC CAGCCCCTCA GCCCTGAGGA GAAACAGGAG 
CTGGACCAGT TGCTCAGTGG TTTGGAAGCA CCAATGCATC GCACAGGTTA CCAAAGCAGC 
CCTGGTGGGG GTGGTGGTGG AGGTGGTGGA GTCCTACATC TCGTCCCTGC CCAGGTACAC 
GTCAATGGAC ACAGCAACAT AGACAGAGAG ACAGATATCC TTGATGATGA CTTGCCCATC 
AGCCAGGAAG GGAACAGTGT GGACAGTCTT GGAACTCTCT CATCTTTTGA GGGCAGAGCC 
ACTCCTGCGG ATCTATACTA CCAGTCAGAG ACAGTCATTA ATGGACAAGA TATGCCCTAC 
TTAGAGAAGA GTGTCCATGA GAAAATGCTA GAGCCCAGAG TTCACAGCTC ATCATCTGTC 
CAAGAGCACT TGGGAGCCCC TAGTGGGTAT GCTAACCAGA ATGGCAACAT CTATCGATCT 
CAGTCATTAG GAGCAACCCC GAACACAGAC CAGAAGTACA TGCCCAAAGC TCCAGCACGC 
ACAACCAGCA GTAGGGATGC CGTGCAGAGA CTGAATGTTT GGCAACAATT TGGTGTACCA 
GAAGTCCCAG TCACAGATGG GGTCACCTTC AACCCTGTAC TACAATCCCA CAGCCTTCCA 
GAGTTCCCTC AGATGGCATC TCAACAAGAG ATTGAGCAGT CCATTGAAGC CCTCAACATG 
CTGATGCTGG ACCTTGATCC AGCACTTACT CAAGTCCCCA AATCCCACAG CGCTCCATCT 
GGAGAAAGTG GTGTGGTCAC CACCCAACCA TCTTTCTCTC AAACCCAAGC ACGCCCATCC 
TACCAGGCTG ACACTGCTAT TCATGACCGC GGTGCGGCCT CTTCAATGGG ATTTCCTTTG 
ACCTTTCAAC AAGTAACTGG AGAGCCTTCA CAACCCACCA TGAAACAGCA GAAACCCACA 
ACTACATCCC AGCATGTCGA ATTCGCTTCT GAAGCAGGAG GTCAGGCCCC CATGTCCCAG 
TCCACAGTGC CTTCCTCCAC CTTTGGAGTT CTCCAGTTGA AACCCCTCAA CATTTATCCC 
TCAAGTACCA CATCCCAGTC CCCCGAGCCT CAGGAGACTC AGCGTAGCTA CTCTTCTTCC 
TCATCCCCTC TTCCCAAAGA GTCTGAACCA GAAGATAGCA GCTACAAATT GGAGGGTCTT 
GTAGCTCACC GTGTAGCTGA GTACACTGCT CGTATCCGCT GCATCAATGA CAGCCAGAGT 
GCTCCCGAGT TTGGCCATCG CCGCCGCTCT CTCTCCTTCA GTGGACTTCC ATCTGGTGAA 
ATGTCGCCAG ATGAGGCTGC AGTTCCTGGA CGCTGTCGGA CCCTTAGTGA GGGCCTTTCC 
CAAGACGAAA GCCCAACTCG AGGAAGCTCA GTCCGCTCCC CCATTCGCTG CATCTCACCA 
GAGCTGGCCA ATACAATTGC TCTGAACCCT GGGGGGCGGC CCAAGGAGAG GCACATGCAC 
AGCTACAGAG AAGCGTTTGA GGAAATGGAT GGGGGTCCCG TTAGCCCCCC TCCAATTGTT 
GGTGGTGAGG TCCTACCCCA AACCCCTGCC TTCCCTGTCT CGCCACAAAC CCCTTACTTC 
AACATGTGTC GATCTCCTCC AGGATTGGCT AAAACACCAC TGTCCTTTCT GGGCCTGAAG 
CCCCACAACC CTGCTGAGAT CCTGCTCAAC CAGACAGGCT CAGAGAGTGA GGATGAGGAA 
GAGCCTCGTA GTTATGTGGA GTCTGTGGCG AAGGCAGCAG CAGTACCTGC ATCTCCAACA 
TATGACAGCG ACAGCTTAGC TCGTCCTGCC GGACCGTCCC ATGCTTACAA TGCTCCACTT 
TCCTCCAGCA GCCCCATTCA GAACCCAGAT CCAGTTGAAA GCACATTGGC TGACAGCGGG 
GTCGGGATGA ATTCTTTATC CCAGGCTGCT GTCGACATTT TCCATTTCCA GCCCTCCGAA 
ATCCCATACC AACCACCACC ACCCCCCTAC CCAACCTCCA TGTTGGGTAG CTACATCAGC 
CCTTCTCCAG CAGAGGCCAT GCAAAATAAT ACCACCACCA TCCAGCCACT TTCTGGAAGT 
CCTGGGACAA GACACCACAC CATCCTTTCT GACATGGCGT CCGTTCCCAA CTTTCAACAC 
AGATTAGCAA ATCAGGATGG TCCCATACTT GGGCGACAGC AGGCTCCAGC TAATGGTCAT 
GTGCCTGGAA TTGCTGGTAG TCCAGTGCAA GGAAGACACA CTATGGTGTC CCAGGGTACA 
CAAAGCAGTC CCATACTGAG CAGACAGTAT CCTGTCACCC AGGGGATTCA GAGTAGTCCA 
ATTCTCAGCA GGCAGCCAAT GGGACAAGCT GTCCAGAGCA GTCCAGTCCT CAACCGACAG 
GCATCCCTGA ATCAAGCAAA CCAAAGCAGT CCAGTGTTGA GTAGACAGCA GTCCATTACC 
CTACCCAACC AAGGCAGCCC GGTCCTAAGC AGACAGTCCT CGCTTAGTCA TCCCAGTCAG 
GGTAGTCCTG TACTAGGACG CCACCCTTCC CTAACACAGG GTAGCCCCAG CCTGGATAGG 
CATCCGATGT ACAGCGGTTA CACCACTCCA GAAGAGCGAC ACGGAGCTCT GTCACGCCAA 
AGCAGCTCTT CAGGATACCA GCCGCCATCT ACACCGTCTT TCCCAGTTTC TCCAGCAGGA 
TACATCGAGG GAGCGGGATT CAGACAGGGG AGTCCTGCTT TCCAACCTCA GTTGCCCGAA 
AAGAGACGCA TGTCCAGCAG TGACCGCACC AATGGTGGCC TGTCCTATGG GACGCTCAAT 
GGGAAGATGT CCTCACCGGT GTCCAGTGGC GGCAGCACTC CGAGTGTTCA TTTCTTCCAC 
ACACTCCCCG ATTTCTCCAA ACTCAACATG TGTGATGGAA GTCCAGAAAC CAGAATGAAC 
GTGAAGTTTG TCCAGGACAC GTCCAAATTC TGGTATAAAC CAGATATCTC GAGAGACCAA 
GCGATTAACA TGCTGAAGGA TCAAGATCCT GGGGCCTTTG TGATTCGGGA CAGTCATTCA 
TTCAGAGGAG CCTACGGCTT GGCTATGAAA GTGGCTTCAC CACCTCCAAC CACTCAACCT 
ACAAAGAAAG CTGGAGATGT CACTAATGAG TTGGTGAGGC ATTTTCTAAT TGAGACGAGT 
CCGAAGGGTG TGAGACTGAA GGGCTGCCCC AACGAGCCGT ACTTTGGCTG CCTGTCTGCG 
CTGGTCTACC AGCACTCCAT CACTCCACTG GCACTGCCCT GCAAACTAGT CATACCAACC 
AGAGATCCTC TGGAAGAGTC ACCTGAGATC GCAACACCAA CCAACCCGGC GGCAGATATG 
CTCAAGCAGG GCGCAGGGCA GAAGAGTTCA GCTGATGGCC ATGCATGCAA TGTGCTGTAC 
ATCAACTCTG TGGATATGGA GTCTTTGACT GGACCTCAAG CCATCGCCAA AGCCATTTCA 
GAAACCATGA ACGCCAGTCC GGCACCCTCA GCCACCATTG TTCACTTCAA GGTGTCGGCA 
CAAGGCATCA CACTCACAGA CAACCAGAGA AAGCTTTTCT TCAGACGTCA TTATCCCATC 
GGCACAGTCA CGTTCTGTGA CATTGATCCA CAGGAGAGGA AGTGGAACAA ACCAGAGGGT 
GGCACTGCCA AATTCTTTGG CTTTGTGGCT AGAAAGCAAG GCAGCACGAC AGACAATGTC 
AGTCACCTGT TTGCTGAGAT GGACCCAGAT CAGCCGGCCA CCGCCATCGT CAACTTTGTC 
TCTAAAGTGA TGATCAGCTC TCAGAAGCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334711.1
CDS	414..5666
Translation	MATAVFQVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAEYTARIRCINDSQSAPEFGHRRRSLSFSGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGEVLPQTPAFPVSPQTPYFNMCRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSESEDEEEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAGQKSSADGHACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_021479036.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479036.1

ORF Insert Sequence:

ATGGCCACCG CTGTGTTTCA GGTCTCGTCC TCCTGTGTTC CTGTCGCCAA CTATGAACTG 
GCCCCCAGCA ATGAGCTCCC CCTGAAACAT ATCGAGACCA CAGGCTCAAC CAAATCCTCC 
AAGAGCATGG AGTCTCGACG CAGACCGTCA AGGAGTTTGA GTTTGATCCA GGCCATGGAG 
GAGAACTATG AGGTGGATCT GGTTTACATC ACCGAGCGCA TCATTTCTGT CACCTTTCCC 
AGTAATGCAG AGGAGCCCAG TTACAGCGCC AACATTAAAG AAGTGGCAAA CATGCTGCGC 
TCCAAACATG GAGACAACTA CCTGCTCTTC AACCTCAGTG AAAAGCGCTT GGATATCTCC 
AAGCTGAATC CCAAAGTTCT GGATTTTGGC TGGCCGGATC ATCACGCTCC TGCTTTGGAT 
AAGATCTGCA GCATCTGCAA AGCCATGGAC ACTTGGATGA ACGCTGGCAG TCACAACGTG 
GTGGTCATAC ACAACAAGGG AAACCGAGGT CGAACTGGGG TGGTTGTGGC TGCGTACATG 
CATTACAGCA ACATATCAGC GAGTGCCGAC CAGGCTCTGG ACAGGTTTGC CATGAAGAGG 
TTTTATGAAG ATAAAGTGCT TCCTGTGGGT CAGCCTTCCC AGAGAAGATA TGTGCAGTAC 
TTCAGCGGTC TGCTCTCCGG CCACATTAAG ATCAACAACA AACCCCTGTT TCTCCATCAC 
GTCATCATGC ACGGCATCCC AAACTTCGAG TCTAAAGGAG GTTGCCGCCC TTTCCTGAAG 
ATCTACCAGG CCATGCAGCC CGTCTACACA TCTGGCATCT ATAATGTCCA AGGAGACAGT 
CAGACTAGTA TCTGCATCAC CATTGAACCG GGCCTGCTTT TGAAAGGAGA CATACTGCTA 
AAGTGTTATC ACAAGCGTTT CCGCAGCCCA TGCCGTGATG TCATATTCAG AGTTCAGTTT 
CACACCTGTG CTGTTCATGA TCTGGGTATC GTCTTCGGCA AAGATGAGCT TGATGAAACC 
TTTAAAGACG ACAGATTTCC AGAATATGGA AAGGTGGAAT TCGTCTTTTC TTTTGGCCCA 
GAAAAAATCC AAGGAATGGA CCACCTTGAG AACGGGCCCA GTGTTTCTGT GGACTACAAT 
ACCCAAGATG CACTAATCCG CTGGGATTCC TACGAGAACT TCAACCAGCG CTGTGAAGAC 
TCCATGGAAG ACGTTATTCA CACCGAAGGT CCATTGGATG GTAGTCTCTA CGCTAAAGTT 
CGTAAGAAAG AGTCTCTGGA AGGAGGGGTT ACAGCCAATG GTCTCCCAGC TGCTGCTGAT 
CACACTTTAC CTGCTGTAGA CCACACTCTA CCACCAGTTG ACCACGCCTT GTCTGTAAGC 
AGTGATTCAG GCAACTCCAC AGCGTCCATC AAGACTGACC GCACTGATGA AGCCAGCCAT 
CAGCCAGCAT TGGTGAGTCA GCCATCTTCC CAGCCCCTCA GCCCTGAGGA GAAACAGGAG 
CTGGACCAGT TGCTCAGTGG TTTGGAAGCA CCAATGCATC GCACAGGTTA CCAAAGCAGC 
CCTGGTGGGG GTGGTGGTGG AGGTGGTGGA GTCCTACATC TCGTCCCTGC CCAGGTACAC 
GTCAATGGAC ACAGCAACAT AGACAGAGAG ACAGATATCC TTGATGATGA CTTGCCCATC 
AGCCAGGAAG GGAACAGTGT GGACAGTCTT GGAACTCTCT CATCTTTTGA GGGCAGAGCC 
ACTCCTGCGG ATCTATACTA CCAGTCAGAG ACAGTCATTA ATGGACAAGA TATGCCCTAC 
TTAGAGAAGA GTGTCCATGA GAAAATGCTA GAGCCCAGAG TTCACAGCTC ATCATCTGTC 
CAAGAGCACT TGGGAGCCCC TAGTGGGTAT GCTAACCAGA ATGGCAACAT CTATCGATCT 
CAGTCATTAG GAGCAACCCC GAACACAGAC CAGAAGTACA TGCCCAAAGC TCCAGCACGC 
ACAACCAGCA GTAGGGATGC CGTGCAGAGA CTGAATGTTT GGCAACAATT TGGTGTACCA 
GAAGTCCCAG TCACAGATGG GGTCACCTTC AACCCTGTAC TACAATCCCA CAGCCTTCCA 
GAGTTCCCTC AGATGGCATC TCAACAAGAG ATTGAGCAGT CCATTGAAGC CCTCAACATG 
CTGATGCTGG ACCTTGATCC AGCACTTACT CAAGTCCCCA AATCCCACAG CGCTCCATCT 
GGAGAAAGTG GTGTGGTCAC CACCCAACCA TCTTTCTCTC AAACCCAAGC ACGCCCATCC 
TACCAGGCTG ACACTGCTAT TCATGACCGC GGTGCGGCCT CTTCAATGGG ATTTCCTTTG 
ACCTTTCAAC AAGTAACTGG AGAGCCTTCA CAACCCACCA TGAAACAGCA GAAACCCACA 
ACTACATCCC AGCATGTCGA ATTCGCTTCT GAAGCAGGAG GTCAGGCCCC CATGTCCCAG 
TCCACAGTGC CTTCCTCCAC CTTTGGAGTT CTCCAGTTGA AACCCCTCAA CATTTATCCC 
TCAAGTACCA CATCCCAGTC CCCCGAGCCT CAGGAGACTC AGCGTAGCTA CTCTTCTTCC 
TCATCCCCTC TTCCCAAAGA GTCTGAACCA GAAGATAGCA GCTACAAATT GGAGGGTCTT 
GTAGCTCACC GTGTAGCTGA GTACACTGCT CGTATCCGCT GCATCAATGA CAGCCAGAGT 
GCTCCCGAGT TTGGCCATCG CCGCCGCTCT CTCTCCTTCA GTGGACTTCC ATCTGGTGAA 
ATGTCGCCAG ATGAGGCTGC AGTTCCTGGA CGCTGTCGGA CCCTTAGTGA GGGCCTTTCC 
CAAGACGAAA GCCCAACTCG AGGAAGCTCA GTCCGCTCCC CCATTCGCTG CATCTCACCA 
GAGCTGGCCA ATACAATTGC TCTGAACCCT GGGGGGCGGC CCAAGGAGAG GCACATGCAC 
AGCTACAGAG AAGCGTTTGA GGAAATGGAT GGGGGTCCCG TTAGCCCCCC TCCAATTGTT 
GGTGGTGAGG TCCTACCCCA AACCCCTGCC TTCCCTGTCT CGCCACAAAC CCCTTACTTC 
AACATGTGTC GATCTCCTCC AGGATTGGCT AAAACACCAC TGTCCTTTCT GGGCCTGAAG 
CCCCACAACC CTGCTGAGAT CCTGCTCAAC CAGACAGGCT CAGAGAGTGA GGATGAGGAA 
GAGCCTCGTA GTTATGTGGA GTCTGTGGCG AAGGCAGCAG CAGTACCTGC ATCTCCAACA 
TATGACAGCG ACAGCTTAGC TCGTCCTGCC GGACCGTCCC ATGCTTACAA TGCTCCACTT 
TCCTCCAGCA GCCCCATTCA GAACCCAGAT CCAGTTGAAA GCACATTGGC TGACAGCGGG 
GTCGGGATGA ATTCTTTATC CCAGGCTGCT GTCGACATTT TCCATTTCCA GCCCTCCGAA 
ATCCCATACC AACCACCACC ACCCCCCTAC CCAACCTCCA TGTTGGGTAG CTACATCAGC 
CCTTCTCCAG CAGAGGCCAT GCAAAATAAT ACCACCACCA TCCAGCCACT TTCTGGAAGT 
CCTGGGACAA GACACCACAC CATCCTTTCT GACATGGCGT CCGTTCCCAA CTTTCAACAC 
AGATTAGCAA ATCAGGATGG TCCCATACTT GGGCGACAGC AGGCTCCAGC TAATGGTCAT 
GTGCCTGGAA TTGCTGGTAG TCCAGTGCAA GGAAGACACA CTATGGTGTC CCAGGGTACA 
CAAAGCAGTC CCATACTGAG CAGACAGTAT CCTGTCACCC AGGGGATTCA GAGTAGTCCA 
ATTCTCAGCA GGCAGCCAAT GGGACAAGCT GTCCAGAGCA GTCCAGTCCT CAACCGACAG 
GCATCCCTGA ATCAAGCAAA CCAAAGCAGT CCAGTGTTGA GTAGACAGCA GTCCATTACC 
CTACCCAACC AAGGCAGCCC GGTCCTAAGC AGACAGTCCT CGCTTAGTCA TCCCAGTCAG 
GGTAGTCCTG TACTAGGACG CCACCCTTCC CTAACACAGG GTAGCCCCAG CCTGGATAGG 
CATCCGATGT ACAGCGGTTA CACCACTCCA GAAGAGCGAC ACGGAGCTCT GTCACGCCAA 
AGCAGCTCTT CAGGATACCA GCCGCCATCT ACACCGTCTT TCCCAGTTTC TCCAGCAGGA 
TACATCGAGG GAGCGGGATT CAGACAGGGG AGTCCTGCTT TCCAACCTCA GTTGCCCGAA 
AAGAGACGCA TGTCCAGCAG TGACCGCACC AATGGTGGCC TGTCCTATGG GACGCTCAAT 
GGGAAGATGT CCTCACCGGT GTCCAGTGGC GGCAGCACTC CGAGTGTTCA TTTCTTCCAC 
ACACTCCCCG ATTTCTCCAA ACTCAACATG TGTGATGGAA GTCCAGAAAC CAGAATGAAC 
GTGAAGTTTG TCCAGGACAC GTCCAAATTC TGGTATAAAC CAGATATCTC GAGAGACCAA 
GCGATTAACA TGCTGAAGGA TCAAGATCCT GGGGCCTTTG TGATTCGGGA CAGTCATTCA 
TTCAGAGGAG CCTACGGCTT GGCTATGAAA GTGGCTTCAC CACCTCCAAC CACTCAACCT 
ACAAAGAAAG CTGGAGATGT CACTAATGAG TTGGTGAGGC ATTTTCTAAT TGAGACGAGT 
CCGAAGGGTG TGAGACTGAA GGGCTGCCCC AACGAGCCGT ACTTTGGCTG CCTGTCTGCG 
CTGGTCTACC AGCACTCCAT CACTCCACTG GCACTGCCCT GCAAACTAGT CATACCAACC 
AGAGATCCTC TGGAAGAGTC ACCTGAGATC GCAACACCAA CCAACCCGGC GGCAGATATG 
CTCAAGCAGG GCGCAGGGCA GAAGAGTTCA GCTGATGGCC ATGCATGCAA TGTGCTGTAC 
ATCAACTCTG TGGATATGGA GTCTTTGACT GGACCTCAAG CCATCGCCAA AGCCATTTCA 
GAAACCATGA ACGCCAGTCC GGCACCCTCA GCCACCATTG TTCACTTCAA GGTGTCGGCA 
CAAGGCATCA CACTCACAGA CAACCAGAGA AAGCTTTTCT TCAGACGTCA TTATCCCATC 
GGCACAGTCA CGTTCTGTGA CATTGATCCA CAGGAGAGGA AGTGGAACAA ACCAGAGGGT 
GGCACTGCCA AATTCTTTGG CTTTGTGGCT AGAAAGCAAG GCAGCACGAC AGACAATGTC 
AGTCACCTGT TTGCTGAGAT GGACCCAGAT CAGCCGGCCA CCGCCATCGT CAACTTTGTC 
TCTAAAGTGA TGATCAGCTC TCAGAAGCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_021479037.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334712.1 Danio rerio tensin 1b (tns1b), transcript variant X11, mRNA.

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CloneID	ODa144123
Clone ID Related Accession (Same CDS sequence)	XM_021479037.1
Accession Version	XM_021479037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA 
GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT 
TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT 
CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC 
CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG 
AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG 
AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT 
AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC 
AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG 
CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG 
ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG 
GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT 
TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT 
TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC 
AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC 
ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC 
TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG 
ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG 
TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC 
ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT 
AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA 
AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC 
CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC 
ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT 
AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC 
ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT 
GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG 
CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG 
GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT 
GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC 
AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC 
CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT 
CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA 
GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA 
GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG 
TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA 
ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA 
GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG 
TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG 
ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA 
GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC 
CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC 
TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT 
ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC 
ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA 
AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA 
TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA 
GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT 
CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG 
TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA 
GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG 
CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC 
TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT 
GGTGAGGTCC TACCCCAAAC CCCTGCCTTC CCTGTCTCGC CACAAACCCC TTACTTCAAC 
ATGTGTCGAT CTCCTCCAGG ATTGGCTAAA ACACCACTGT CCTTTCTGGG CCTGAAGCCC 
CACAACCCTG CTGAGATCCT GCTCAACCAG ACAGGCTCAG AGCCTCGTAG TTATGTGGAG 
TCTGTGGCGA AGGCAGCAGC AGTACCTGCA TCTCCAACAT ATGACAGCGA CAGCTTAGCT 
CGTCCTGCCG GACCGTCCCA TGCTTACAAT GCTCCACTTT CCTCCAGCAG CCCCATTCAG 
AACCCAGATC CAGTTGAAAG CACATTGGCT GACAGCGGGG TCGGGATGAA TTCTTTATCC 
CAGGCTGCTG TCGACATTTT CCATTTCCAG CCCTCCGAAA TCCCATACCA ACCACCACCA 
CCCCCCTACC CAACCTCCAT GTTGGGTAGC TACATCAGCC CTTCTCCAGC AGAGGCCATG 
CAAAATAATA CCACCACCAT CCAGCCACTT TCTGGAAGTC CTGGGACAAG ACACCACACC 
ATCCTTTCTG ACATGGCGTC CGTTCCCAAC TTTCAACACA GATTAGCAAA TCAGGATGGT 
CCCATACTTG GGCGACAGCA GGCTCCAGCT AATGGTCATG TGCCTGGAAT TGCTGGTAGT 
CCAGTGCAAG GAAGACACAC TATGGTGTCC CAGGGTACAC AAAGCAGTCC CATACTGAGC 
AGACAGTATC CTGTCACCCA GGGGATTCAG AGTAGTCCAA TTCTCAGCAG GCAGCCAATG 
GGACAAGCTG TCCAGAGCAG TCCAGTCCTC AACCGACAGG CATCCCTGAA TCAAGCAAAC 
CAAAGCAGTC CAGTGTTGAG TAGACAGCAG TCCATTACCC TACCCAACCA AGGCAGCCCG 
GTCCTAAGCA GACAGTCCTC GCTTAGTCAT CCCAGTCAGG GTAGTCCTGT ACTAGGACGC 
CACCCTTCCC TAACACAGGG TAGCCCCAGC CTGGATAGGC ATCCGATGTA CAGCGGTTAC 
ACCACTCCAG AAGAGCGACA CGGAGCTCTG TCACGCCAAA GCAGCTCTTC AGGATACCAG 
CCGCCATCTA CACCGTCTTT CCCAGTTTCT CCAGCAGGAT ACATCGAGGG AGCGGGATTC 
AGACAGGGGA GTCCTGCTTT CCAACCTCAG TTGCCCGAAA AGAGACGCAT GTCCAGCAGT 
GACCGCACCA ATGGTGGCCT GTCCTATGGG ACGCTCAATG GGAAGATGTC CTCACCGGTG 
TCCAGTGGCG GCAGCACTCC GAGTGTTCAT TTCTTCCACA CACTCCCCGA TTTCTCCAAA 
CTCAACATGT GTGATGGAAG TCCAGAAACC AGAATGAACG TGAAGTTTGT CCAGGACACG 
TCCAAATTCT GGTATAAACC AGATATCTCG AGAGACCAAG CGATTAACAT GCTGAAGGAT 
CAAGATCCTG GGGCCTTTGT GATTCGGGAC AGTCATTCAT TCAGAGGAGC CTACGGCTTG 
GCTATGAAAG TGGCTTCACC ACCTCCAACC ACTCAACCTA CAAAGAAAGC TGGAGATGTC 
ACTAATGAGT TGGTGAGGCA TTTTCTAATT GAGACGAGTC CGAAGGGTGT GAGACTGAAG 
GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC CTGTCTGCGC TGGTCTACCA GCACTCCATC 
ACTCCACTGG CACTGCCCTG CAAACTAGTC ATACCAACCA GAGATCCTCT GGAAGAGTCA 
CCTGAGATCG CAACACCAAC CAACCCGGCG GCAGATATGC TCAAGCAGGG CGCAGCATGC 
AATGTGCTGT ACATCAACTC TGTGGATATG GAGTCTTTGA CTGGACCTCA AGCCATCGCC 
AAAGCCATTT CAGAAACCAT GAACGCCAGT CCGGCACCCT CAGCCACCAT TGTTCACTTC 
AAGGTGTCGG CACAAGGCAT CACACTCACA GACAACCAGA GAAAGCTTTT CTTCAGACGT 
CATTATCCCA TCGGCACAGT CACGTTCTGT GACATTGATC CACAGGAGAG GAAGTGGAAC 
AAACCAGAGG GTGGCACTGC CAAATTCTTT GGCTTTGTGG CTAGAAAGCA AGGCAGCACG 
ACAGACAATG TCAGTCACCT GTTTGCTGAG ATGGACCCAG ATCAGCCGGC CACCGCCATC 
GTCAACTTTG TCTCTAAAGT GATGATCAGC TCTCAGAAGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334712.1
CDS	284..5728
Translation	MGCTVSFICCEDDFLQMPLDQHVEKKKEEKRLLFKPEEFEAVHSHTFRLKTFKKGKHCGVCKQTVNNEGLICRVCRLACHRKCEVKVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAEYTARIRCINDSQSAPEFGHRRRSLSFSGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGEVLPQTPAFPVSPQTPYFNMCRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_021479037.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479037.1

ORF Insert Sequence:

ATGGGATGCA CCGTGAGCTT CATCTGCTGT GAAGACGATT TTCTGCAGAT GCCTTTGGAT 
CAGCATGTGG AGAAAAAGAA AGAGGAAAAG AGGCTGCTAT TTAAGCCTGA AGAGTTTGAA 
GCCGTTCATT CGCACACCTT CCGGCTGAAG ACGTTCAAGA AGGGGAAACA CTGTGGTGTT 
TGCAAGCAGA CGGTCAACAA TGAGGGGCTC ATCTGCAGAG TTTGCCGGTT AGCCTGTCAT 
CGGAAGTGTG AAGTCAAGGT CTCGTCCTCC TGTGTTCCTG TCGCCAACTA TGAACTGGCC 
CCCAGCAATG AGCTCCCCCT GAAACATATC GAGACCACAG GCTCAACCAA ATCCTCCAAG 
AGCATGGAGT CTCGACGCAG ACCGTCAAGG AGTTTGAGTT TGATCCAGGC CATGGAGGAG 
AACTATGAGG TGGATCTGGT TTACATCACC GAGCGCATCA TTTCTGTCAC CTTTCCCAGT 
AATGCAGAGG AGCCCAGTTA CAGCGCCAAC ATTAAAGAAG TGGCAAACAT GCTGCGCTCC 
AAACATGGAG ACAACTACCT GCTCTTCAAC CTCAGTGAAA AGCGCTTGGA TATCTCCAAG 
CTGAATCCCA AAGTTCTGGA TTTTGGCTGG CCGGATCATC ACGCTCCTGC TTTGGATAAG 
ATCTGCAGCA TCTGCAAAGC CATGGACACT TGGATGAACG CTGGCAGTCA CAACGTGGTG 
GTCATACACA ACAAGGGAAA CCGAGGTCGA ACTGGGGTGG TTGTGGCTGC GTACATGCAT 
TACAGCAACA TATCAGCGAG TGCCGACCAG GCTCTGGACA GGTTTGCCAT GAAGAGGTTT 
TATGAAGATA AAGTGCTTCC TGTGGGTCAG CCTTCCCAGA GAAGATATGT GCAGTACTTC 
AGCGGTCTGC TCTCCGGCCA CATTAAGATC AACAACAAAC CCCTGTTTCT CCATCACGTC 
ATCATGCACG GCATCCCAAA CTTCGAGTCT AAAGGAGGTT GCCGCCCTTT CCTGAAGATC 
TACCAGGCCA TGCAGCCCGT CTACACATCT GGCATCTATA ATGTCCAAGG AGACAGTCAG 
ACTAGTATCT GCATCACCAT TGAACCGGGC CTGCTTTTGA AAGGAGACAT ACTGCTAAAG 
TGTTATCACA AGCGTTTCCG CAGCCCATGC CGTGATGTCA TATTCAGAGT TCAGTTTCAC 
ACCTGTGCTG TTCATGATCT GGGTATCGTC TTCGGCAAAG ATGAGCTTGA TGAAACCTTT 
AAAGACGACA GATTTCCAGA ATATGGAAAG GTGGAATTCG TCTTTTCTTT TGGCCCAGAA 
AAAATCCAAG GAATGGACCA CCTTGAGAAC GGGCCCAGTG TTTCTGTGGA CTACAATACC 
CAAGATGCAC TAATCCGCTG GGATTCCTAC GAGAACTTCA ACCAGCGCTG TGAAGACTCC 
ATGGAAGACG TTATTCACAC CGAAGGTCCA TTGGATGGTA GTCTCTACGC TAAAGTTCGT 
AAGAAAGAGT CTCTGGAAGG AGGGGTTACA GCCAATGGTC TCCCAGCTGC TGCTGATCAC 
ACTTTACCTG CTGTAGACCA CACTCTACCA CCAGTTGACC ACGCCTTGTC TGTAAGCAGT 
GATTCAGGCA ACTCCACAGC GTCCATCAAG ACTGACCGCA CTGATGAAGC CAGCCATCAG 
CCAGCATTGG TGAGTCAGCC ATCTTCCCAG CCCCTCAGCC CTGAGGAGAA ACAGGAGCTG 
GACCAGTTGC TCAGTGGTTT GGAAGCACCA ATGCATCGCA CAGGTTACCA AAGCAGCCCT 
GGTGGGGGTG GTGGTGGAGG TGGTGGAGTC CTACATCTCG TCCCTGCCCA GGTACACGTC 
AATGGACACA GCAACATAGA CAGAGAGACA GATATCCTTG ATGATGACTT GCCCATCAGC 
CAGGAAGGGA ACAGTGTGGA CAGTCTTGGA ACTCTCTCAT CTTTTGAGGG CAGAGCCACT 
CCTGCGGATC TATACTACCA GTCAGAGACA GTCATTAATG GACAAGATAT GCCCTACTTA 
GAGAAGAGTG TCCATGAGAA AATGCTAGAG CCCAGAGTTC ACAGCTCATC ATCTGTCCAA 
GAGCACTTGG GAGCCCCTAG TGGGTATGCT AACCAGAATG GCAACATCTA TCGATCTCAG 
TCATTAGGAG CAACCCCGAA CACAGACCAG AAGTACATGC CCAAAGCTCC AGCACGCACA 
ACCAGCAGTA GGGATGCCGT GCAGAGACTG AATGTTTGGC AACAATTTGG TGTACCAGAA 
GTCCCAGTCA CAGATGGGGT CACCTTCAAC CCTGTACTAC AATCCCACAG CCTTCCAGAG 
TTCCCTCAGA TGGCATCTCA ACAAGAGATT GAGCAGTCCA TTGAAGCCCT CAACATGCTG 
ATGCTGGACC TTGATCCAGC ACTTACTCAA GTCCCCAAAT CCCACAGCGC TCCATCTGGA 
GAAAGTGGTG TGGTCACCAC CCAACCATCT TTCTCTCAAA CCCAAGCACG CCCATCCTAC 
CAGGCTGACA CTGCTATTCA TGACCGCGGT GCGGCCTCTT CAATGGGATT TCCTTTGACC 
TTTCAACAAG TAACTGGAGA GCCTTCACAA CCCACCATGA AACAGCAGAA ACCCACAACT 
ACATCCCAGC ATGTCGAATT CGCTTCTGAA GCAGGAGGTC AGGCCCCCAT GTCCCAGTCC 
ACAGTGCCTT CCTCCACCTT TGGAGTTCTC CAGTTGAAAC CCCTCAACAT TTATCCCTCA 
AGTACCACAT CCCAGTCCCC CGAGCCTCAG GAGACTCAGC GTAGCTACTC TTCTTCCTCA 
TCCCCTCTTC CCAAAGAGTC TGAACCAGAA GATAGCAGCT ACAAATTGGA GGGTCTTGTA 
GCTCACCGTG TAGCTGAGTA CACTGCTCGT ATCCGCTGCA TCAATGACAG CCAGAGTGCT 
CCCGAGTTTG GCCATCGCCG CCGCTCTCTC TCCTTCAGTG GACTTCCATC TGGTGAAATG 
TCGCCAGATG AGGCTGCAGT TCCTGGACGC TGTCGGACCC TTAGTGAGGG CCTTTCCCAA 
GACGAAAGCC CAACTCGAGG AAGCTCAGTC CGCTCCCCCA TTCGCTGCAT CTCACCAGAG 
CTGGCCAATA CAATTGCTCT GAACCCTGGG GGGCGGCCCA AGGAGAGGCA CATGCACAGC 
TACAGAGAAG CGTTTGAGGA AATGGATGGG GGTCCCGTTA GCCCCCCTCC AATTGTTGGT 
GGTGAGGTCC TACCCCAAAC CCCTGCCTTC CCTGTCTCGC CACAAACCCC TTACTTCAAC 
ATGTGTCGAT CTCCTCCAGG ATTGGCTAAA ACACCACTGT CCTTTCTGGG CCTGAAGCCC 
CACAACCCTG CTGAGATCCT GCTCAACCAG ACAGGCTCAG AGCCTCGTAG TTATGTGGAG 
TCTGTGGCGA AGGCAGCAGC AGTACCTGCA TCTCCAACAT ATGACAGCGA CAGCTTAGCT 
CGTCCTGCCG GACCGTCCCA TGCTTACAAT GCTCCACTTT CCTCCAGCAG CCCCATTCAG 
AACCCAGATC CAGTTGAAAG CACATTGGCT GACAGCGGGG TCGGGATGAA TTCTTTATCC 
CAGGCTGCTG TCGACATTTT CCATTTCCAG CCCTCCGAAA TCCCATACCA ACCACCACCA 
CCCCCCTACC CAACCTCCAT GTTGGGTAGC TACATCAGCC CTTCTCCAGC AGAGGCCATG 
CAAAATAATA CCACCACCAT CCAGCCACTT TCTGGAAGTC CTGGGACAAG ACACCACACC 
ATCCTTTCTG ACATGGCGTC CGTTCCCAAC TTTCAACACA GATTAGCAAA TCAGGATGGT 
CCCATACTTG GGCGACAGCA GGCTCCAGCT AATGGTCATG TGCCTGGAAT TGCTGGTAGT 
CCAGTGCAAG GAAGACACAC TATGGTGTCC CAGGGTACAC AAAGCAGTCC CATACTGAGC 
AGACAGTATC CTGTCACCCA GGGGATTCAG AGTAGTCCAA TTCTCAGCAG GCAGCCAATG 
GGACAAGCTG TCCAGAGCAG TCCAGTCCTC AACCGACAGG CATCCCTGAA TCAAGCAAAC 
CAAAGCAGTC CAGTGTTGAG TAGACAGCAG TCCATTACCC TACCCAACCA AGGCAGCCCG 
GTCCTAAGCA GACAGTCCTC GCTTAGTCAT CCCAGTCAGG GTAGTCCTGT ACTAGGACGC 
CACCCTTCCC TAACACAGGG TAGCCCCAGC CTGGATAGGC ATCCGATGTA CAGCGGTTAC 
ACCACTCCAG AAGAGCGACA CGGAGCTCTG TCACGCCAAA GCAGCTCTTC AGGATACCAG 
CCGCCATCTA CACCGTCTTT CCCAGTTTCT CCAGCAGGAT ACATCGAGGG AGCGGGATTC 
AGACAGGGGA GTCCTGCTTT CCAACCTCAG TTGCCCGAAA AGAGACGCAT GTCCAGCAGT 
GACCGCACCA ATGGTGGCCT GTCCTATGGG ACGCTCAATG GGAAGATGTC CTCACCGGTG 
TCCAGTGGCG GCAGCACTCC GAGTGTTCAT TTCTTCCACA CACTCCCCGA TTTCTCCAAA 
CTCAACATGT GTGATGGAAG TCCAGAAACC AGAATGAACG TGAAGTTTGT CCAGGACACG 
TCCAAATTCT GGTATAAACC AGATATCTCG AGAGACCAAG CGATTAACAT GCTGAAGGAT 
CAAGATCCTG GGGCCTTTGT GATTCGGGAC AGTCATTCAT TCAGAGGAGC CTACGGCTTG 
GCTATGAAAG TGGCTTCACC ACCTCCAACC ACTCAACCTA CAAAGAAAGC TGGAGATGTC 
ACTAATGAGT TGGTGAGGCA TTTTCTAATT GAGACGAGTC CGAAGGGTGT GAGACTGAAG 
GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC CTGTCTGCGC TGGTCTACCA GCACTCCATC 
ACTCCACTGG CACTGCCCTG CAAACTAGTC ATACCAACCA GAGATCCTCT GGAAGAGTCA 
CCTGAGATCG CAACACCAAC CAACCCGGCG GCAGATATGC TCAAGCAGGG CGCAGCATGC 
AATGTGCTGT ACATCAACTC TGTGGATATG GAGTCTTTGA CTGGACCTCA AGCCATCGCC 
AAAGCCATTT CAGAAACCAT GAACGCCAGT CCGGCACCCT CAGCCACCAT TGTTCACTTC 
AAGGTGTCGG CACAAGGCAT CACACTCACA GACAACCAGA GAAAGCTTTT CTTCAGACGT 
CATTATCCCA TCGGCACAGT CACGTTCTGT GACATTGATC CACAGGAGAG GAAGTGGAAC 
AAACCAGAGG GTGGCACTGC CAAATTCTTT GGCTTTGTGG CTAGAAAGCA AGGCAGCACG 
ACAGACAATG TCAGTCACCT GTTTGCTGAG ATGGACCCAG ATCAGCCGGC CACCGCCATC 
GTCAACTTTG TCTCTAAAGT GATGATCAGC TCTCAGAAGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_017357846.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213335.1 Danio rerio tensin 1b (tns1b), transcript variant X1, mRNA.

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CloneID	ODa60375
Clone ID Related Accession (Same CDS sequence)	XM_017357846.1
Accession Version	XM_017357846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tensin-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG 
CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG 
GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG 
TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA 
CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG 
TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC 
ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC 
GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC 
TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT 
GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG 
GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA 
GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC 
GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG 
GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT 
AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC 
GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC 
ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA 
CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC 
CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT 
ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT 
GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT 
GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT 
TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA 
GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG 
GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT 
CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC 
ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT 
TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA 
GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT 
GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA 
GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT 
CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA 
GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG 
CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG 
TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA 
GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG 
AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC 
TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA 
GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT 
ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA 
CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC 
CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT 
TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT 
TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA 
GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG 
CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA 
CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA 
TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG 
GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC 
ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG 
CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT 
CCAATTGTTG GTGGTGAGGT CCTACCCCAA ACCCCTGCCT TCCCTGTCTC GCCACAAACC 
CCTTACTTCA ACATGTGTCG ATCTCCTCCA GGATTGGCTA AAACACCACT GTCCTTTCTG 
GGCCTGAAGC CCCACAACCC TGCTGAGATC CTGCTCAACC AGACAGGCTC AGAGAGTGAG 
GATGAGGAAG AGCCTCGTAG TTATGTGGAG TCTGTGGCGA AGGCAGCAGC AGTACCTGCA 
TCTCCAACAT ATGACAGCGA CAGCTTAGCT CGTCCTGCCG GACCGTCCCA TGCTTACAAT 
GCTCCACTTT CCTCCAGCAG CCCCATTCAG AACCCAGATC CAGTTGAAAG CACATTGGCT 
GACAGCGGGG TCGGGATGAA TTCTTTATCC CAGGCTGCTG TCGACATTTT CCATTTCCAG 
CCCTCCGAAA TCCCATACCA ACCACCACCA CCCCCCTACC CAACCTCCAT GTTGGGTAGC 
TACATCAGCC CTTCTCCAGC AGAGGCCATG CAAAATAATA CCACCACCAT CCAGCCACTT 
TCTGGAAGTC CTGGGACAAG ACACCACACC ATCCTTTCTG ACATGGCGTC CGTTCCCAAC 
TTTCAACACA GATTAGCAAA TCAGGATGGT CCCATACTTG GGCGACAGCA GGCTCCAGCT 
AATGGTCATG TGCCTGGAAT TGCTGGTAGT CCAGTGCAAG GAAGACACAC TATGGTGTCC 
CAGGGTACAC AAAGCAGTCC CATACTGAGC AGACAGTATC CTGTCACCCA GGGGATTCAG 
AGTAGTCCAA TTCTCAGCAG GCAGCCAATG GGACAAGCTG TCCAGAGCAG TCCAGTCCTC 
AACCGACAGG CATCCCTGAA TCAAGCAAAC CAAAGCAGTC CAGTGTTGAG TAGACAGCAG 
TCCATTACCC TACCCAACCA AGGCAGCCCG GTCCTAAGCA GACAGTCCTC GCTTAGTCAT 
CCCAGTCAGG GTAGTCCTGT ACTAGGACGC CACCCTTCCC TAACACAGGG TAGCCCCAGC 
CTGGATAGGC ATCCGATGTA CAGCGGTTAC ACCACTCCAG AAGAGCGACA CGGAGCTCTG 
TCACGCCAAA GCAGCTCTTC AGGATACCAG CCGCCATCTA CACCGTCTTT CCCAGTTTCT 
CCAGCAGGAT ACATCGAGGG AGCGGGATTC AGACAGGGGA GTCCTGCTTT CCAACCTCAG 
TTGCCCGAAA AGAGACGCAT GTCCAGCAGT GACCGCACCA ATGGTGGCCT GTCCTATGGG 
ACGCTCAATG GGAAGATGTC CTCACCGGTG TCCAGTGGCG GCAGCACTCC GAGTGTTCAT 
TTCTTCCACA CACTCCCCGA TTTCTCCAAA CTCAACATGT GTGATGGAAG TCCAGAAACC 
AGAATGAACG TGAAGTTTGT CCAGGACACG TCCAAATTCT GGTATAAACC AGATATCTCG 
AGAGACCAAG CGATTAACAT GCTGAAGGAT CAAGATCCTG GGGCCTTTGT GATTCGGGAC 
AGTCATTCAT TCAGAGGAGC CTACGGCTTG GCTATGAAAG TGGCTTCACC ACCTCCAACC 
ACTCAACCTA CAAAGAAAGC TGGAGATGTC ACTAATGAGT TGGTGAGGCA TTTTCTAATT 
GAGACGAGTC CGAAGGGTGT GAGACTGAAG GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC 
CTGTCTGCGC TGGTCTACCA GCACTCCATC ACTCCACTGG CACTGCCCTG CAAACTAGTC 
ATACCAACCA GAGATCCTCT GGAAGAGTCA CCTGAGATCG CAACACCAAC CAACCCGGCG 
GCAGATATGC TCAAGCAGGG CGCAGGGCAG AAGAGTTCAG CTGATGGCCA TGCATGCAAT 
GTGCTGTACA TCAACTCTGT GGATATGGAG TCTTTGACTG GACCTCAAGC CATCGCCAAA 
GCCATTTCAG AAACCATGAA CGCCAGTCCG GCACCCTCAG CCACCATTGT TCACTTCAAG 
GTGTCGGCAC AAGGCATCAC ACTCACAGAC AACCAGAGAA AGCTTTTCTT CAGACGTCAT 
TATCCCATCG GCACAGTCAC GTTCTGTGAC ATTGATCCAC AGGAGAGGAA GTGGAACAAA 
CCAGAGGGTG GCACTGCCAA ATTCTTTGGC TTTGTGGCTA GAAAGCAAGG CAGCACGACA 
GACAATGTCA GTCACCTGTT TGCTGAGATG GACCCAGATC AGCCGGCCAC CGCCATCGTC 
AACTTTGTCT CTAAAGTGAT GATCAGCTCT CAGAAGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213335.1
CDS	715..6096
Translation	MPSVSLSLPAALAGRARTWVCLSCMFWPEEFEAVHSHTFRLKTFKKGKHCGVCKQTVNNEGLICRVCRLACHRKCEVKVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGEVLPQTPAFPVSPQTPYFNMCRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSESEDEEEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAGQKSSADGHACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_017357846.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357846.1

ORF Insert Sequence:

ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG 
CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG 
GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG 
TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA 
CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG 
TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC 
ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC 
GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC 
TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT 
GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG 
GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA 
GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC 
GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG 
GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT 
AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC 
GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC 
ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA 
CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC 
CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT 
ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT 
GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT 
GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT 
TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA 
GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG 
GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT 
CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC 
ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT 
TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA 
GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT 
GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA 
GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT 
CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA 
GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG 
CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG 
TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA 
GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG 
AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC 
TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA 
GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT 
ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA 
CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC 
CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT 
TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT 
TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA 
GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG 
CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA 
CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA 
TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG 
GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC 
ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG 
CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT 
CCAATTGTTG GTGGTGAGGT CCTACCCCAA ACCCCTGCCT TCCCTGTCTC GCCACAAACC 
CCTTACTTCA ACATGTGTCG ATCTCCTCCA GGATTGGCTA AAACACCACT GTCCTTTCTG 
GGCCTGAAGC CCCACAACCC TGCTGAGATC CTGCTCAACC AGACAGGCTC AGAGAGTGAG 
GATGAGGAAG AGCCTCGTAG TTATGTGGAG TCTGTGGCGA AGGCAGCAGC AGTACCTGCA 
TCTCCAACAT ATGACAGCGA CAGCTTAGCT CGTCCTGCCG GACCGTCCCA TGCTTACAAT 
GCTCCACTTT CCTCCAGCAG CCCCATTCAG AACCCAGATC CAGTTGAAAG CACATTGGCT 
GACAGCGGGG TCGGGATGAA TTCTTTATCC CAGGCTGCTG TCGACATTTT CCATTTCCAG 
CCCTCCGAAA TCCCATACCA ACCACCACCA CCCCCCTACC CAACCTCCAT GTTGGGTAGC 
TACATCAGCC CTTCTCCAGC AGAGGCCATG CAAAATAATA CCACCACCAT CCAGCCACTT 
TCTGGAAGTC CTGGGACAAG ACACCACACC ATCCTTTCTG ACATGGCGTC CGTTCCCAAC 
TTTCAACACA GATTAGCAAA TCAGGATGGT CCCATACTTG GGCGACAGCA GGCTCCAGCT 
AATGGTCATG TGCCTGGAAT TGCTGGTAGT CCAGTGCAAG GAAGACACAC TATGGTGTCC 
CAGGGTACAC AAAGCAGTCC CATACTGAGC AGACAGTATC CTGTCACCCA GGGGATTCAG 
AGTAGTCCAA TTCTCAGCAG GCAGCCAATG GGACAAGCTG TCCAGAGCAG TCCAGTCCTC 
AACCGACAGG CATCCCTGAA TCAAGCAAAC CAAAGCAGTC CAGTGTTGAG TAGACAGCAG 
TCCATTACCC TACCCAACCA AGGCAGCCCG GTCCTAAGCA GACAGTCCTC GCTTAGTCAT 
CCCAGTCAGG GTAGTCCTGT ACTAGGACGC CACCCTTCCC TAACACAGGG TAGCCCCAGC 
CTGGATAGGC ATCCGATGTA CAGCGGTTAC ACCACTCCAG AAGAGCGACA CGGAGCTCTG 
TCACGCCAAA GCAGCTCTTC AGGATACCAG CCGCCATCTA CACCGTCTTT CCCAGTTTCT 
CCAGCAGGAT ACATCGAGGG AGCGGGATTC AGACAGGGGA GTCCTGCTTT CCAACCTCAG 
TTGCCCGAAA AGAGACGCAT GTCCAGCAGT GACCGCACCA ATGGTGGCCT GTCCTATGGG 
ACGCTCAATG GGAAGATGTC CTCACCGGTG TCCAGTGGCG GCAGCACTCC GAGTGTTCAT 
TTCTTCCACA CACTCCCCGA TTTCTCCAAA CTCAACATGT GTGATGGAAG TCCAGAAACC 
AGAATGAACG TGAAGTTTGT CCAGGACACG TCCAAATTCT GGTATAAACC AGATATCTCG 
AGAGACCAAG CGATTAACAT GCTGAAGGAT CAAGATCCTG GGGCCTTTGT GATTCGGGAC 
AGTCATTCAT TCAGAGGAGC CTACGGCTTG GCTATGAAAG TGGCTTCACC ACCTCCAACC 
ACTCAACCTA CAAAGAAAGC TGGAGATGTC ACTAATGAGT TGGTGAGGCA TTTTCTAATT 
GAGACGAGTC CGAAGGGTGT GAGACTGAAG GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC 
CTGTCTGCGC TGGTCTACCA GCACTCCATC ACTCCACTGG CACTGCCCTG CAAACTAGTC 
ATACCAACCA GAGATCCTCT GGAAGAGTCA CCTGAGATCG CAACACCAAC CAACCCGGCG 
GCAGATATGC TCAAGCAGGG CGCAGGGCAG AAGAGTTCAG CTGATGGCCA TGCATGCAAT 
GTGCTGTACA TCAACTCTGT GGATATGGAG TCTTTGACTG GACCTCAAGC CATCGCCAAA 
GCCATTTCAG AAACCATGAA CGCCAGTCCG GCACCCTCAG CCACCATTGT TCACTTCAAG 
GTGTCGGCAC AAGGCATCAC ACTCACAGAC AACCAGAGAA AGCTTTTCTT CAGACGTCAT 
TATCCCATCG GCACAGTCAC GTTCTGTGAC ATTGATCCAC AGGAGAGGAA GTGGAACAAA 
CCAGAGGGTG GCACTGCCAA ATTCTTTGGC TTTGTGGCTA GAAAGCAAGG CAGCACGACA 
GACAATGTCA GTCACCTGTT TGCTGAGATG GACCCAGATC AGCCGGCCAC CGCCATCGTC 
AACTTTGTCT CTAAAGTGAT GATCAGCTCT CAGAAGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_017357847.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213336.1 Danio rerio tensin 1b (tns1b), transcript variant X2, mRNA.

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CloneID	ODa60376
Clone ID Related Accession (Same CDS sequence)	XM_017357847.1
Accession Version	XM_017357847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tensin-1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGAGT CCAGTTCTTC TTTGGGATTA AATATTCGGG GGCTAACAAG ACGAGTCGGT 
GAGATGATGT CCGGCTGGAC GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG 
CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG 
GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG 
TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA 
CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG 
TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC 
ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC 
GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC 
TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT 
GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG 
GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA 
GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC 
GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG 
GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT 
AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC 
GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC 
ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA 
CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC 
CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT 
ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT 
GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT 
GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT 
TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA 
GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG 
GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT 
CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC 
ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT 
TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA 
GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT 
GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA 
GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT 
CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA 
GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG 
CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG 
TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA 
GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG 
AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC 
TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA 
GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT 
ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA 
CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC 
CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT 
TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT 
TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA 
GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG 
CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA 
CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA 
TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG 
GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC 
ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG 
CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT 
CCAATTGTTG GTGGTGAGGT CCTACCCCAA ACCCCTGCCT TCCCTGTCTC GCCACAAACC 
CCTTACTTCA ACATGTGTCG ATCTCCTCCA GGATTGGCTA AAACACCACT GTCCTTTCTG 
GGCCTGAAGC CCCACAACCC TGCTGAGATC CTGCTCAACC AGACAGGCTC AGAGAGTGAG 
GATGAGGAAG AGCCTCGTAG TTATGTGGAG TCTGTGGCGA AGGCAGCAGC AGTACCTGCA 
TCTCCAACAT ATGACAGCGA CAGCTTAGCT CGTCCTGCCG GACCGTCCCA TGCTTACAAT 
GCTCCACTTT CCTCCAGCAG CCCCATTCAG AACCCAGATC CAGTTGAAAG CACATTGGCT 
GACAGCGGGG TCGGGATGAA TTCTTTATCC CAGGCTGCTG TCGACATTTT CCATTTCCAG 
CCCTCCGAAA TCCCATACCA ACCACCACCA CCCCCCTACC CAACCTCCAT GTTGGGTAGC 
TACATCAGCC CTTCTCCAGC AGAGGCCATG CAAAATAATA CCACCACCAT CCAGCCACTT 
TCTGGAAGTC CTGGGACAAG ACACCACACC ATCCTTTCTG ACATGGCGTC CGTTCCCAAC 
TTTCAACACA GATTAGCAAA TCAGGATGGT CCCATACTTG GGCGACAGCA GGCTCCAGCT 
AATGGTCATG TGCCTGGAAT TGCTGGTAGT CCAGTGCAAG GAAGACACAC TATGGTGTCC 
CAGGGTACAC AAAGCAGTCC CATACTGAGC AGACAGTATC CTGTCACCCA GGGGATTCAG 
AGTAGTCCAA TTCTCAGCAG GCAGCCAATG GGACAAGCTG TCCAGAGCAG TCCAGTCCTC 
AACCGACAGG CATCCCTGAA TCAAGCAAAC CAAAGCAGTC CAGTGTTGAG TAGACAGCAG 
TCCATTACCC TACCCAACCA AGGCAGCCCG GTCCTAAGCA GACAGTCCTC GCTTAGTCAT 
CCCAGTCAGG GTAGTCCTGT ACTAGGACGC CACCCTTCCC TAACACAGGG TAGCCCCAGC 
CTGGATAGGC ATCCGATGTA CAGCGGTTAC ACCACTCCAG AAGAGCGACA CGGAGCTCTG 
TCACGCCAAA GCAGCTCTTC AGGATACCAG CCGCCATCTA CACCGTCTTT CCCAGTTTCT 
CCAGCAGGAT ACATCGAGGG AGCGGGATTC AGACAGGGGA GTCCTGCTTT CCAACCTCAG 
TTGCCCGAAA AGAGACGCAT GTCCAGCAGT GACCGCACCA ATGGTGGCCT GTCCTATGGG 
ACGCTCAATG GGAAGATGTC CTCACCGGTG TCCAGTGGCG GCAGCACTCC GAGTGTTCAT 
TTCTTCCACA CACTCCCCGA TTTCTCCAAA CTCAACATGT GTGATGGAAG TCCAGAAACC 
AGAATGAACG TGAAGTTTGT CCAGGACACG TCCAAATTCT GGTATAAACC AGATATCTCG 
AGAGACCAAG CGATTAACAT GCTGAAGGAT CAAGATCCTG GGGCCTTTGT GATTCGGGAC 
AGTCATTCAT TCAGAGGAGC CTACGGCTTG GCTATGAAAG TGGCTTCACC ACCTCCAACC 
ACTCAACCTA CAAAGAAAGC TGGAGATGTC ACTAATGAGT TGGTGAGGCA TTTTCTAATT 
GAGACGAGTC CGAAGGGTGT GAGACTGAAG GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC 
CTGTCTGCGC TGGTCTACCA GCACTCCATC ACTCCACTGG CACTGCCCTG CAAACTAGTC 
ATACCAACCA GAGATCCTCT GGAAGAGTCA CCTGAGATCG CAACACCAAC CAACCCGGCG 
GCAGATATGC TCAAGCAGGG CGCAGGGCAG AAGAGTTCAG CTGATGGCCA TGCATGCAAT 
GTGCTGTACA TCAACTCTGT GGATATGGAG TCTTTGACTG GACCTCAAGC CATCGCCAAA 
GCCATTTCAG AAACCATGAA CGCCAGTCCG GCACCCTCAG CCACCATTGT TCACTTCAAG 
GTGTCGGCAC AAGGCATCAC ACTCACAGAC AACCAGAGAA AGCTTTTCTT CAGACGTCAT 
TATCCCATCG GCACAGTCAC GTTCTGTGAC ATTGATCCAC AGGAGAGGAA GTGGAACAAA 
CCAGAGGGTG GCACTGCCAA ATTCTTTGGC TTTGTGGCTA GAAAGCAAGG CAGCACGACA 
GACAATGTCA GTCACCTGTT TGCTGAGATG GACCCAGATC AGCCGGCCAC CGCCATCGTC 
AACTTTGTCT CTAAAGTGAT GATCAGCTCT CAGAAGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213336.1
CDS	119..5500
Translation	MLESSSSLGLNIRGLTRRVGEMMSGWTPEEFEAVHSHTFRLKTFKKGKHCGVCKQTVNNEGLICRVCRLACHRKCEVKVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGEVLPQTPAFPVSPQTPYFNMCRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSESEDEEEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAGQKSSADGHACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_017357847.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357847.1

ORF Insert Sequence:

ATGCTTGAGT CCAGTTCTTC TTTGGGATTA AATATTCGGG GGCTAACAAG ACGAGTCGGT 
GAGATGATGT CCGGCTGGAC GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG 
CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG 
GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG 
TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA 
CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG 
TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC 
ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC 
GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC 
TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT 
GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG 
GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA 
GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC 
GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG 
GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT 
AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC 
GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC 
ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA 
CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC 
CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT 
ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT 
GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT 
GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT 
TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA 
GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG 
GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT 
CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC 
ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT 
TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA 
GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT 
GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA 
GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT 
CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA 
GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG 
CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG 
TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA 
GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG 
AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC 
TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA 
GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT 
ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA 
CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC 
CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT 
TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT 
TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA 
GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG 
CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA 
CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA 
TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG 
GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC 
ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG 
CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT 
CCAATTGTTG GTGGTGAGGT CCTACCCCAA ACCCCTGCCT TCCCTGTCTC GCCACAAACC 
CCTTACTTCA ACATGTGTCG ATCTCCTCCA GGATTGGCTA AAACACCACT GTCCTTTCTG 
GGCCTGAAGC CCCACAACCC TGCTGAGATC CTGCTCAACC AGACAGGCTC AGAGAGTGAG 
GATGAGGAAG AGCCTCGTAG TTATGTGGAG TCTGTGGCGA AGGCAGCAGC AGTACCTGCA 
TCTCCAACAT ATGACAGCGA CAGCTTAGCT CGTCCTGCCG GACCGTCCCA TGCTTACAAT 
GCTCCACTTT CCTCCAGCAG CCCCATTCAG AACCCAGATC CAGTTGAAAG CACATTGGCT 
GACAGCGGGG TCGGGATGAA TTCTTTATCC CAGGCTGCTG TCGACATTTT CCATTTCCAG 
CCCTCCGAAA TCCCATACCA ACCACCACCA CCCCCCTACC CAACCTCCAT GTTGGGTAGC 
TACATCAGCC CTTCTCCAGC AGAGGCCATG CAAAATAATA CCACCACCAT CCAGCCACTT 
TCTGGAAGTC CTGGGACAAG ACACCACACC ATCCTTTCTG ACATGGCGTC CGTTCCCAAC 
TTTCAACACA GATTAGCAAA TCAGGATGGT CCCATACTTG GGCGACAGCA GGCTCCAGCT 
AATGGTCATG TGCCTGGAAT TGCTGGTAGT CCAGTGCAAG GAAGACACAC TATGGTGTCC 
CAGGGTACAC AAAGCAGTCC CATACTGAGC AGACAGTATC CTGTCACCCA GGGGATTCAG 
AGTAGTCCAA TTCTCAGCAG GCAGCCAATG GGACAAGCTG TCCAGAGCAG TCCAGTCCTC 
AACCGACAGG CATCCCTGAA TCAAGCAAAC CAAAGCAGTC CAGTGTTGAG TAGACAGCAG 
TCCATTACCC TACCCAACCA AGGCAGCCCG GTCCTAAGCA GACAGTCCTC GCTTAGTCAT 
CCCAGTCAGG GTAGTCCTGT ACTAGGACGC CACCCTTCCC TAACACAGGG TAGCCCCAGC 
CTGGATAGGC ATCCGATGTA CAGCGGTTAC ACCACTCCAG AAGAGCGACA CGGAGCTCTG 
TCACGCCAAA GCAGCTCTTC AGGATACCAG CCGCCATCTA CACCGTCTTT CCCAGTTTCT 
CCAGCAGGAT ACATCGAGGG AGCGGGATTC AGACAGGGGA GTCCTGCTTT CCAACCTCAG 
TTGCCCGAAA AGAGACGCAT GTCCAGCAGT GACCGCACCA ATGGTGGCCT GTCCTATGGG 
ACGCTCAATG GGAAGATGTC CTCACCGGTG TCCAGTGGCG GCAGCACTCC GAGTGTTCAT 
TTCTTCCACA CACTCCCCGA TTTCTCCAAA CTCAACATGT GTGATGGAAG TCCAGAAACC 
AGAATGAACG TGAAGTTTGT CCAGGACACG TCCAAATTCT GGTATAAACC AGATATCTCG 
AGAGACCAAG CGATTAACAT GCTGAAGGAT CAAGATCCTG GGGCCTTTGT GATTCGGGAC 
AGTCATTCAT TCAGAGGAGC CTACGGCTTG GCTATGAAAG TGGCTTCACC ACCTCCAACC 
ACTCAACCTA CAAAGAAAGC TGGAGATGTC ACTAATGAGT TGGTGAGGCA TTTTCTAATT 
GAGACGAGTC CGAAGGGTGT GAGACTGAAG GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC 
CTGTCTGCGC TGGTCTACCA GCACTCCATC ACTCCACTGG CACTGCCCTG CAAACTAGTC 
ATACCAACCA GAGATCCTCT GGAAGAGTCA CCTGAGATCG CAACACCAAC CAACCCGGCG 
GCAGATATGC TCAAGCAGGG CGCAGGGCAG AAGAGTTCAG CTGATGGCCA TGCATGCAAT 
GTGCTGTACA TCAACTCTGT GGATATGGAG TCTTTGACTG GACCTCAAGC CATCGCCAAA 
GCCATTTCAG AAACCATGAA CGCCAGTCCG GCACCCTCAG CCACCATTGT TCACTTCAAG 
GTGTCGGCAC AAGGCATCAC ACTCACAGAC AACCAGAGAA AGCTTTTCTT CAGACGTCAT 
TATCCCATCG GCACAGTCAC GTTCTGTGAC ATTGATCCAC AGGAGAGGAA GTGGAACAAA 
CCAGAGGGTG GCACTGCCAA ATTCTTTGGC TTTGTGGCTA GAAAGCAAGG CAGCACGACA 
GACAATGTCA GTCACCTGTT TGCTGAGATG GACCCAGATC AGCCGGCCAC CGCCATCGTC 
AACTTTGTCT CTAAAGTGAT GATCAGCTCT CAGAAGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_017357848.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213337.1 Danio rerio tensin 1b (tns1b), transcript variant X3, mRNA.

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CloneID	ODa60377
Clone ID Related Accession (Same CDS sequence)	XM_017357848.1
Accession Version	XM_017357848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tensin-1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG 
CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG 
GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG 
TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA 
CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG 
TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC 
ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC 
GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC 
TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT 
GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG 
GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA 
GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC 
GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG 
GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT 
AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC 
GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC 
ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA 
CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC 
CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT 
ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT 
GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT 
GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT 
TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA 
GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG 
GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT 
CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC 
ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT 
TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA 
GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT 
GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA 
GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT 
CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA 
GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG 
CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG 
TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA 
GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG 
AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC 
TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA 
GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT 
ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA 
CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC 
CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT 
TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT 
TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA 
GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG 
CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA 
CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA 
TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG 
GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC 
ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG 
CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT 
CCAATTGTTG GTGGTGAGGT CCTACCCCAA ACCCCTGCCT TCCCTGTCTC GCCACAAACC 
CCTTACTTCA ACATGTGTCG ATCTCCTCCA GGATTGGCTA AAACACCACT GTCCTTTCTG 
GGCCTGAAGC CCCACAACCC TGCTGAGATC CTGCTCAACC AGACAGGCTC AGAGCCTCGT 
AGTTATGTGG AGTCTGTGGC GAAGGCAGCA GCAGTACCTG CATCTCCAAC ATATGACAGC 
GACAGCTTAG CTCGTCCTGC CGGACCGTCC CATGCTTACA ATGCTCCACT TTCCTCCAGC 
AGCCCCATTC AGAACCCAGA TCCAGTTGAA AGCACATTGG CTGACAGCGG GGTCGGGATG 
AATTCTTTAT CCCAGGCTGC TGTCGACATT TTCCATTTCC AGCCCTCCGA AATCCCATAC 
CAACCACCAC CACCCCCCTA CCCAACCTCC ATGTTGGGTA GCTACATCAG CCCTTCTCCA 
GCAGAGGCCA TGCAAAATAA TACCACCACC ATCCAGCCAC TTTCTGGAAG TCCTGGGACA 
AGACACCACA CCATCCTTTC TGACATGGCG TCCGTTCCCA ACTTTCAACA CAGATTAGCA 
AATCAGGATG GTCCCATACT TGGGCGACAG CAGGCTCCAG CTAATGGTCA TGTGCCTGGA 
ATTGCTGGTA GTCCAGTGCA AGGAAGACAC ACTATGGTGT CCCAGGGTAC ACAAAGCAGT 
CCCATACTGA GCAGACAGTA TCCTGTCACC CAGGGGATTC AGAGTAGTCC AATTCTCAGC 
AGGCAGCCAA TGGGACAAGC TGTCCAGAGC AGTCCAGTCC TCAACCGACA GGCATCCCTG 
AATCAAGCAA ACCAAAGCAG TCCAGTGTTG AGTAGACAGC AGTCCATTAC CCTACCCAAC 
CAAGGCAGCC CGGTCCTAAG CAGACAGTCC TCGCTTAGTC ATCCCAGTCA GGGTAGTCCT 
GTACTAGGAC GCCACCCTTC CCTAACACAG GGTAGCCCCA GCCTGGATAG GCATCCGATG 
TACAGCGGTT ACACCACTCC AGAAGAGCGA CACGGAGCTC TGTCACGCCA AAGCAGCTCT 
TCAGGATACC AGCCGCCATC TACACCGTCT TTCCCAGTTT CTCCAGCAGG ATACATCGAG 
GGAGCGGGAT TCAGACAGGG GAGTCCTGCT TTCCAACCTC AGTTGCCCGA AAAGAGACGC 
ATGTCCAGCA GTGACCGCAC CAATGGTGGC CTGTCCTATG GGACGCTCAA TGGGAAGATG 
TCCTCACCGG TGTCCAGTGG CGGCAGCACT CCGAGTGTTC ATTTCTTCCA CACACTCCCC 
GATTTCTCCA AACTCAACAT GTGTGATGGA AGTCCAGAAA CCAGAATGAA CGTGAAGTTT 
GTCCAGGACA CGTCCAAATT CTGGTATAAA CCAGATATCT CGAGAGACCA AGCGATTAAC 
ATGCTGAAGG ATCAAGATCC TGGGGCCTTT GTGATTCGGG ACAGTCATTC ATTCAGAGGA 
GCCTACGGCT TGGCTATGAA AGTGGCTTCA CCACCTCCAA CCACTCAACC TACAAAGAAA 
GCTGGAGATG TCACTAATGA GTTGGTGAGG CATTTTCTAA TTGAGACGAG TCCGAAGGGT 
GTGAGACTGA AGGGCTGCCC CAACGAGCCG TACTTTGGCT GCCTGTCTGC GCTGGTCTAC 
CAGCACTCCA TCACTCCACT GGCACTGCCC TGCAAACTAG TCATACCAAC CAGAGATCCT 
CTGGAAGAGT CACCTGAGAT CGCAACACCA ACCAACCCGG CGGCAGATAT GCTCAAGCAG 
GGCGCAGGGC AGAAGAGTTC AGCTGATGGC CATGCATGCA ATGTGCTGTA CATCAACTCT 
GTGGATATGG AGTCTTTGAC TGGACCTCAA GCCATCGCCA AAGCCATTTC AGAAACCATG 
AACGCCAGTC CGGCACCCTC AGCCACCATT GTTCACTTCA AGGTGTCGGC ACAAGGCATC 
ACACTCACAG ACAACCAGAG AAAGCTTTTC TTCAGACGTC ATTATCCCAT CGGCACAGTC 
ACGTTCTGTG ACATTGATCC ACAGGAGAGG AAGTGGAACA AACCAGAGGG TGGCACTGCC 
AAATTCTTTG GCTTTGTGGC TAGAAAGCAA GGCAGCACGA CAGACAATGT CAGTCACCTG 
TTTGCTGAGA TGGACCCAGA TCAGCCGGCC ACCGCCATCG TCAACTTTGT CTCTAAAGTG 
ATGATCAGCT CTCAGAAGCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213337.1
CDS	714..6077
Translation	MPSVSLSLPAALAGRARTWVCLSCMFWPEEFEAVHSHTFRLKTFKKGKHCGVCKQTVNNEGLICRVCRLACHRKCEVKVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGEVLPQTPAFPVSPQTPYFNMCRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAGQKSSADGHACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_017357848.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357848.1

ORF Insert Sequence:

ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG 
CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG 
GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG 
TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA 
CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG 
TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC 
ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC 
GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC 
TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT 
GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG 
GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA 
GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC 
GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG 
GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT 
AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC 
GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC 
ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA 
CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC 
CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT 
ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT 
GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT 
GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT 
TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA 
GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG 
GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT 
CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC 
ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT 
TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA 
GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT 
GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA 
GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT 
CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA 
GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG 
CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG 
TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA 
GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG 
AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC 
TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA 
GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT 
ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA 
CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC 
CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT 
TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT 
TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA 
GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG 
CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA 
CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA 
TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG 
GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC 
ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG 
CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT 
CCAATTGTTG GTGGTGAGGT CCTACCCCAA ACCCCTGCCT TCCCTGTCTC GCCACAAACC 
CCTTACTTCA ACATGTGTCG ATCTCCTCCA GGATTGGCTA AAACACCACT GTCCTTTCTG 
GGCCTGAAGC CCCACAACCC TGCTGAGATC CTGCTCAACC AGACAGGCTC AGAGCCTCGT 
AGTTATGTGG AGTCTGTGGC GAAGGCAGCA GCAGTACCTG CATCTCCAAC ATATGACAGC 
GACAGCTTAG CTCGTCCTGC CGGACCGTCC CATGCTTACA ATGCTCCACT TTCCTCCAGC 
AGCCCCATTC AGAACCCAGA TCCAGTTGAA AGCACATTGG CTGACAGCGG GGTCGGGATG 
AATTCTTTAT CCCAGGCTGC TGTCGACATT TTCCATTTCC AGCCCTCCGA AATCCCATAC 
CAACCACCAC CACCCCCCTA CCCAACCTCC ATGTTGGGTA GCTACATCAG CCCTTCTCCA 
GCAGAGGCCA TGCAAAATAA TACCACCACC ATCCAGCCAC TTTCTGGAAG TCCTGGGACA 
AGACACCACA CCATCCTTTC TGACATGGCG TCCGTTCCCA ACTTTCAACA CAGATTAGCA 
AATCAGGATG GTCCCATACT TGGGCGACAG CAGGCTCCAG CTAATGGTCA TGTGCCTGGA 
ATTGCTGGTA GTCCAGTGCA AGGAAGACAC ACTATGGTGT CCCAGGGTAC ACAAAGCAGT 
CCCATACTGA GCAGACAGTA TCCTGTCACC CAGGGGATTC AGAGTAGTCC AATTCTCAGC 
AGGCAGCCAA TGGGACAAGC TGTCCAGAGC AGTCCAGTCC TCAACCGACA GGCATCCCTG 
AATCAAGCAA ACCAAAGCAG TCCAGTGTTG AGTAGACAGC AGTCCATTAC CCTACCCAAC 
CAAGGCAGCC CGGTCCTAAG CAGACAGTCC TCGCTTAGTC ATCCCAGTCA GGGTAGTCCT 
GTACTAGGAC GCCACCCTTC CCTAACACAG GGTAGCCCCA GCCTGGATAG GCATCCGATG 
TACAGCGGTT ACACCACTCC AGAAGAGCGA CACGGAGCTC TGTCACGCCA AAGCAGCTCT 
TCAGGATACC AGCCGCCATC TACACCGTCT TTCCCAGTTT CTCCAGCAGG ATACATCGAG 
GGAGCGGGAT TCAGACAGGG GAGTCCTGCT TTCCAACCTC AGTTGCCCGA AAAGAGACGC 
ATGTCCAGCA GTGACCGCAC CAATGGTGGC CTGTCCTATG GGACGCTCAA TGGGAAGATG 
TCCTCACCGG TGTCCAGTGG CGGCAGCACT CCGAGTGTTC ATTTCTTCCA CACACTCCCC 
GATTTCTCCA AACTCAACAT GTGTGATGGA AGTCCAGAAA CCAGAATGAA CGTGAAGTTT 
GTCCAGGACA CGTCCAAATT CTGGTATAAA CCAGATATCT CGAGAGACCA AGCGATTAAC 
ATGCTGAAGG ATCAAGATCC TGGGGCCTTT GTGATTCGGG ACAGTCATTC ATTCAGAGGA 
GCCTACGGCT TGGCTATGAA AGTGGCTTCA CCACCTCCAA CCACTCAACC TACAAAGAAA 
GCTGGAGATG TCACTAATGA GTTGGTGAGG CATTTTCTAA TTGAGACGAG TCCGAAGGGT 
GTGAGACTGA AGGGCTGCCC CAACGAGCCG TACTTTGGCT GCCTGTCTGC GCTGGTCTAC 
CAGCACTCCA TCACTCCACT GGCACTGCCC TGCAAACTAG TCATACCAAC CAGAGATCCT 
CTGGAAGAGT CACCTGAGAT CGCAACACCA ACCAACCCGG CGGCAGATAT GCTCAAGCAG 
GGCGCAGGGC AGAAGAGTTC AGCTGATGGC CATGCATGCA ATGTGCTGTA CATCAACTCT 
GTGGATATGG AGTCTTTGAC TGGACCTCAA GCCATCGCCA AAGCCATTTC AGAAACCATG 
AACGCCAGTC CGGCACCCTC AGCCACCATT GTTCACTTCA AGGTGTCGGC ACAAGGCATC 
ACACTCACAG ACAACCAGAG AAAGCTTTTC TTCAGACGTC ATTATCCCAT CGGCACAGTC 
ACGTTCTGTG ACATTGATCC ACAGGAGAGG AAGTGGAACA AACCAGAGGG TGGCACTGCC 
AAATTCTTTG GCTTTGTGGC TAGAAAGCAA GGCAGCACGA CAGACAATGT CAGTCACCTG 
TTTGCTGAGA TGGACCCAGA TCAGCCGGCC ACCGCCATCG TCAACTTTGT CTCTAAAGTG 
ATGATCAGCT CTCAGAAGCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_017357849.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213338.1 Danio rerio tensin 1b (tns1b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa60378
Clone ID Related Accession (Same CDS sequence)	XM_017357849.1
Accession Version	XM_017357849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tensin-1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG 
CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG 
GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG 
TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA 
CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG 
TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC 
ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC 
GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC 
TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT 
GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG 
GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA 
GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC 
GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG 
GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT 
AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC 
GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC 
ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA 
CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC 
CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT 
ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT 
GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT 
GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT 
TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA 
GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG 
GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT 
CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC 
ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT 
TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA 
GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT 
GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA 
GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT 
CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA 
GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG 
CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG 
TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA 
GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG 
AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC 
TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA 
GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT 
ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA 
CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC 
CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT 
TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT 
TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA 
GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG 
CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA 
CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA 
TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG 
GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC 
ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG 
CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT 
CCAATTGTTG GTGGTGAGGT CCTACCCCAA ACCCCTGCCT TCCCTGTCTC GCCACAAACC 
CCTTACTTCA ACATGTGTCG ATCTCCTCCA GGATTGGCTA AAACACCACT GTCCTTTCTG 
GGCCTGAAGC CCCACAACCC TGCTGAGATC CTGCTCAACC AGACAGGCTC AGAGAGTGAG 
GATGAGGAAG AGCCTCGTAG TTATGTGGAG TCTGTGGCGA AGGCAGCAGC AGTACCTGCA 
TCTCCAACAT ATGACAGCGA CAGCTTAGCT CGTCCTGCCG GACCGTCCCA TGCTTACAAT 
GCTCCACTTT CCTCCAGCAG CCCCATTCAG AACCCAGATC CAGTTGAAAG CACATTGGCT 
GACAGCGGGG TCGGGATGAA TTCTTTATCC CAGGCTGCTG TCGACATTTT CCATTTCCAG 
CCCTCCGAAA TCCCATACCA ACCACCACCA CCCCCCTACC CAACCTCCAT GTTGGGTAGC 
TACATCAGCC CTTCTCCAGC AGAGGCCATG CAAAATAATA CCACCACCAT CCAGCCACTT 
TCTGGAAGTC CTGGGACAAG ACACCACACC ATCCTTTCTG ACATGGCGTC CGTTCCCAAC 
TTTCAACACA GATTAGCAAA TCAGGATGGT CCCATACTTG GGCGACAGCA GGCTCCAGCT 
AATGGTCATG TGCCTGGAAT TGCTGGTAGT CCAGTGCAAG GAAGACACAC TATGGTGTCC 
CAGGGTACAC AAAGCAGTCC CATACTGAGC AGACAGTATC CTGTCACCCA GGGGATTCAG 
AGTAGTCCAA TTCTCAGCAG GCAGCCAATG GGACAAGCTG TCCAGAGCAG TCCAGTCCTC 
AACCGACAGG CATCCCTGAA TCAAGCAAAC CAAAGCAGTC CAGTGTTGAG TAGACAGCAG 
TCCATTACCC TACCCAACCA AGGCAGCCCG GTCCTAAGCA GACAGTCCTC GCTTAGTCAT 
CCCAGTCAGG GTAGTCCTGT ACTAGGACGC CACCCTTCCC TAACACAGGG TAGCCCCAGC 
CTGGATAGGC ATCCGATGTA CAGCGGTTAC ACCACTCCAG AAGAGCGACA CGGAGCTCTG 
TCACGCCAAA GCAGCTCTTC AGGATACCAG CCGCCATCTA CACCGTCTTT CCCAGTTTCT 
CCAGCAGGAT ACATCGAGGG AGCGGGATTC AGACAGGGGA GTCCTGCTTT CCAACCTCAG 
TTGCCCGAAA AGAGACGCAT GTCCAGCAGT GACCGCACCA ATGGTGGCCT GTCCTATGGG 
ACGCTCAATG GGAAGATGTC CTCACCGGTG TCCAGTGGCG GCAGCACTCC GAGTGTTCAT 
TTCTTCCACA CACTCCCCGA TTTCTCCAAA CTCAACATGT GTGATGGAAG TCCAGAAACC 
AGAATGAACG TGAAGTTTGT CCAGGACACG TCCAAATTCT GGTATAAACC AGATATCTCG 
AGAGACCAAG CGATTAACAT GCTGAAGGAT CAAGATCCTG GGGCCTTTGT GATTCGGGAC 
AGTCATTCAT TCAGAGGAGC CTACGGCTTG GCTATGAAAG TGGCTTCACC ACCTCCAACC 
ACTCAACCTA CAAAGAAAGC TGGAGATGTC ACTAATGAGT TGGTGAGGCA TTTTCTAATT 
GAGACGAGTC CGAAGGGTGT GAGACTGAAG GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC 
CTGTCTGCGC TGGTCTACCA GCACTCCATC ACTCCACTGG CACTGCCCTG CAAACTAGTC 
ATACCAACCA GAGATCCTCT GGAAGAGTCA CCTGAGATCG CAACACCAAC CAACCCGGCG 
GCAGATATGC TCAAGCAGGG CGCAGCATGC AATGTGCTGT ACATCAACTC TGTGGATATG 
GAGTCTTTGA CTGGACCTCA AGCCATCGCC AAAGCCATTT CAGAAACCAT GAACGCCAGT 
CCGGCACCCT CAGCCACCAT TGTTCACTTC AAGGTGTCGG CACAAGGCAT CACACTCACA 
GACAACCAGA GAAAGCTTTT CTTCAGACGT CATTATCCCA TCGGCACAGT CACGTTCTGT 
GACATTGATC CACAGGAGAG GAAGTGGAAC AAACCAGAGG GTGGCACTGC CAAATTCTTT 
GGCTTTGTGG CTAGAAAGCA AGGCAGCACG ACAGACAATG TCAGTCACCT GTTTGCTGAG 
ATGGACCCAG ATCAGCCGGC CACCGCCATC GTCAACTTTG TCTCTAAAGT GATGATCAGC 
TCTCAGAAGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213338.1
CDS	714..6068
Translation	MPSVSLSLPAALAGRARTWVCLSCMFWPEEFEAVHSHTFRLKTFKKGKHCGVCKQTVNNEGLICRVCRLACHRKCEVKVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGEVLPQTPAFPVSPQTPYFNMCRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSESEDEEEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_017357849.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357849.1

ORF Insert Sequence:

ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG 
CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG 
GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG 
TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA 
CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG 
TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC 
ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC 
GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC 
TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT 
GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG 
GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA 
GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC 
GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG 
GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT 
AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC 
GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC 
ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA 
CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC 
CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT 
ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT 
GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT 
GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT 
TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA 
GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG 
GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT 
CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC 
ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT 
TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA 
GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT 
GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA 
GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT 
CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA 
GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG 
CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG 
TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA 
GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG 
AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC 
TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA 
GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT 
ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA 
CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC 
CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT 
TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT 
TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA 
GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG 
CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA 
CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA 
TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG 
GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC 
ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG 
CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT 
CCAATTGTTG GTGGTGAGGT CCTACCCCAA ACCCCTGCCT TCCCTGTCTC GCCACAAACC 
CCTTACTTCA ACATGTGTCG ATCTCCTCCA GGATTGGCTA AAACACCACT GTCCTTTCTG 
GGCCTGAAGC CCCACAACCC TGCTGAGATC CTGCTCAACC AGACAGGCTC AGAGAGTGAG 
GATGAGGAAG AGCCTCGTAG TTATGTGGAG TCTGTGGCGA AGGCAGCAGC AGTACCTGCA 
TCTCCAACAT ATGACAGCGA CAGCTTAGCT CGTCCTGCCG GACCGTCCCA TGCTTACAAT 
GCTCCACTTT CCTCCAGCAG CCCCATTCAG AACCCAGATC CAGTTGAAAG CACATTGGCT 
GACAGCGGGG TCGGGATGAA TTCTTTATCC CAGGCTGCTG TCGACATTTT CCATTTCCAG 
CCCTCCGAAA TCCCATACCA ACCACCACCA CCCCCCTACC CAACCTCCAT GTTGGGTAGC 
TACATCAGCC CTTCTCCAGC AGAGGCCATG CAAAATAATA CCACCACCAT CCAGCCACTT 
TCTGGAAGTC CTGGGACAAG ACACCACACC ATCCTTTCTG ACATGGCGTC CGTTCCCAAC 
TTTCAACACA GATTAGCAAA TCAGGATGGT CCCATACTTG GGCGACAGCA GGCTCCAGCT 
AATGGTCATG TGCCTGGAAT TGCTGGTAGT CCAGTGCAAG GAAGACACAC TATGGTGTCC 
CAGGGTACAC AAAGCAGTCC CATACTGAGC AGACAGTATC CTGTCACCCA GGGGATTCAG 
AGTAGTCCAA TTCTCAGCAG GCAGCCAATG GGACAAGCTG TCCAGAGCAG TCCAGTCCTC 
AACCGACAGG CATCCCTGAA TCAAGCAAAC CAAAGCAGTC CAGTGTTGAG TAGACAGCAG 
TCCATTACCC TACCCAACCA AGGCAGCCCG GTCCTAAGCA GACAGTCCTC GCTTAGTCAT 
CCCAGTCAGG GTAGTCCTGT ACTAGGACGC CACCCTTCCC TAACACAGGG TAGCCCCAGC 
CTGGATAGGC ATCCGATGTA CAGCGGTTAC ACCACTCCAG AAGAGCGACA CGGAGCTCTG 
TCACGCCAAA GCAGCTCTTC AGGATACCAG CCGCCATCTA CACCGTCTTT CCCAGTTTCT 
CCAGCAGGAT ACATCGAGGG AGCGGGATTC AGACAGGGGA GTCCTGCTTT CCAACCTCAG 
TTGCCCGAAA AGAGACGCAT GTCCAGCAGT GACCGCACCA ATGGTGGCCT GTCCTATGGG 
ACGCTCAATG GGAAGATGTC CTCACCGGTG TCCAGTGGCG GCAGCACTCC GAGTGTTCAT 
TTCTTCCACA CACTCCCCGA TTTCTCCAAA CTCAACATGT GTGATGGAAG TCCAGAAACC 
AGAATGAACG TGAAGTTTGT CCAGGACACG TCCAAATTCT GGTATAAACC AGATATCTCG 
AGAGACCAAG CGATTAACAT GCTGAAGGAT CAAGATCCTG GGGCCTTTGT GATTCGGGAC 
AGTCATTCAT TCAGAGGAGC CTACGGCTTG GCTATGAAAG TGGCTTCACC ACCTCCAACC 
ACTCAACCTA CAAAGAAAGC TGGAGATGTC ACTAATGAGT TGGTGAGGCA TTTTCTAATT 
GAGACGAGTC CGAAGGGTGT GAGACTGAAG GGCTGCCCCA ACGAGCCGTA CTTTGGCTGC 
CTGTCTGCGC TGGTCTACCA GCACTCCATC ACTCCACTGG CACTGCCCTG CAAACTAGTC 
ATACCAACCA GAGATCCTCT GGAAGAGTCA CCTGAGATCG CAACACCAAC CAACCCGGCG 
GCAGATATGC TCAAGCAGGG CGCAGCATGC AATGTGCTGT ACATCAACTC TGTGGATATG 
GAGTCTTTGA CTGGACCTCA AGCCATCGCC AAAGCCATTT CAGAAACCAT GAACGCCAGT 
CCGGCACCCT CAGCCACCAT TGTTCACTTC AAGGTGTCGG CACAAGGCAT CACACTCACA 
GACAACCAGA GAAAGCTTTT CTTCAGACGT CATTATCCCA TCGGCACAGT CACGTTCTGT 
GACATTGATC CACAGGAGAG GAAGTGGAAC AAACCAGAGG GTGGCACTGC CAAATTCTTT 
GGCTTTGTGG CTAGAAAGCA AGGCAGCACG ACAGACAATG TCAGTCACCT GTTTGCTGAG 
ATGGACCCAG ATCAGCCGGC CACCGCCATC GTCAACTTTG TCTCTAAAGT GATGATCAGC 
TCTCAGAAGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_009304969.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303244.2 Danio rerio tensin 1b (tns1b), transcript variant X5, mRNA.

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CloneID	ODa60379
Clone ID Related Accession (Same CDS sequence)	XM_009304969.2
Accession Version	XM_009304969.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tensin-1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG 
CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG 
GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG 
TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA 
CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG 
TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC 
ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC 
GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC 
TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT 
GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG 
GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA 
GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC 
GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG 
GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT 
AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC 
GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC 
ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA 
CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC 
CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT 
ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT 
GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT 
GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT 
TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA 
GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG 
GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT 
CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC 
ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT 
TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA 
GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT 
GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA 
GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT 
CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA 
GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG 
CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG 
TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA 
GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG 
AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC 
TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA 
GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT 
ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA 
CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC 
CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT 
TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT 
TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA 
GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG 
CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA 
CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA 
TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG 
GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC 
ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG 
CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT 
CCAATTGTTG GTGGTCGATC TCCTCCAGGA TTGGCTAAAA CACCACTGTC CTTTCTGGGC 
CTGAAGCCCC ACAACCCTGC TGAGATCCTG CTCAACCAGA CAGGCTCAGA GAGTGAGGAT 
GAGGAAGAGC CTCGTAGTTA TGTGGAGTCT GTGGCGAAGG CAGCAGCAGT ACCTGCATCT 
CCAACATATG ACAGCGACAG CTTAGCTCGT CCTGCCGGAC CGTCCCATGC TTACAATGCT 
CCACTTTCCT CCAGCAGCCC CATTCAGAAC CCAGATCCAG TTGAAAGCAC ATTGGCTGAC 
AGCGGGGTCG GGATGAATTC TTTATCCCAG GCTGCTGTCG ACATTTTCCA TTTCCAGCCC 
TCCGAAATCC CATACCAACC ACCACCACCC CCCTACCCAA CCTCCATGTT GGGTAGCTAC 
ATCAGCCCTT CTCCAGCAGA GGCCATGCAA AATAATACCA CCACCATCCA GCCACTTTCT 
GGAAGTCCTG GGACAAGACA CCACACCATC CTTTCTGACA TGGCGTCCGT TCCCAACTTT 
CAACACAGAT TAGCAAATCA GGATGGTCCC ATACTTGGGC GACAGCAGGC TCCAGCTAAT 
GGTCATGTGC CTGGAATTGC TGGTAGTCCA GTGCAAGGAA GACACACTAT GGTGTCCCAG 
GGTACACAAA GCAGTCCCAT ACTGAGCAGA CAGTATCCTG TCACCCAGGG GATTCAGAGT 
AGTCCAATTC TCAGCAGGCA GCCAATGGGA CAAGCTGTCC AGAGCAGTCC AGTCCTCAAC 
CGACAGGCAT CCCTGAATCA AGCAAACCAA AGCAGTCCAG TGTTGAGTAG ACAGCAGTCC 
ATTACCCTAC CCAACCAAGG CAGCCCGGTC CTAAGCAGAC AGTCCTCGCT TAGTCATCCC 
AGTCAGGGTA GTCCTGTACT AGGACGCCAC CCTTCCCTAA CACAGGGTAG CCCCAGCCTG 
GATAGGCATC CGATGTACAG CGGTTACACC ACTCCAGAAG AGCGACACGG AGCTCTGTCA 
CGCCAAAGCA GCTCTTCAGG ATACCAGCCG CCATCTACAC CGTCTTTCCC AGTTTCTCCA 
GCAGGATACA TCGAGGGAGC GGGATTCAGA CAGGGGAGTC CTGCTTTCCA ACCTCAGTTG 
CCCGAAAAGA GACGCATGTC CAGCAGTGAC CGCACCAATG GTGGCCTGTC CTATGGGACG 
CTCAATGGGA AGATGTCCTC ACCGGTGTCC AGTGGCGGCA GCACTCCGAG TGTTCATTTC 
TTCCACACAC TCCCCGATTT CTCCAAACTC AACATGTGTG ATGGAAGTCC AGAAACCAGA 
ATGAACGTGA AGTTTGTCCA GGACACGTCC AAATTCTGGT ATAAACCAGA TATCTCGAGA 
GACCAAGCGA TTAACATGCT GAAGGATCAA GATCCTGGGG CCTTTGTGAT TCGGGACAGT 
CATTCATTCA GAGGAGCCTA CGGCTTGGCT ATGAAAGTGG CTTCACCACC TCCAACCACT 
CAACCTACAA AGAAAGCTGG AGATGTCACT AATGAGTTGG TGAGGCATTT TCTAATTGAG 
ACGAGTCCGA AGGGTGTGAG ACTGAAGGGC TGCCCCAACG AGCCGTACTT TGGCTGCCTG 
TCTGCGCTGG TCTACCAGCA CTCCATCACT CCACTGGCAC TGCCCTGCAA ACTAGTCATA 
CCAACCAGAG ATCCTCTGGA AGAGTCACCT GAGATCGCAA CACCAACCAA CCCGGCGGCA 
GATATGCTCA AGCAGGGCGC AGGGCAGAAG AGTTCAGCTG ATGGCCATGC ATGCAATGTG 
CTGTACATCA ACTCTGTGGA TATGGAGTCT TTGACTGGAC CTCAAGCCAT CGCCAAAGCC 
ATTTCAGAAA CCATGAACGC CAGTCCGGCA CCCTCAGCCA CCATTGTTCA CTTCAAGGTG 
TCGGCACAAG GCATCACACT CACAGACAAC CAGAGAAAGC TTTTCTTCAG ACGTCATTAT 
CCCATCGGCA CAGTCACGTT CTGTGACATT GATCCACAGG AGAGGAAGTG GAACAAACCA 
GAGGGTGGCA CTGCCAAATT CTTTGGCTTT GTGGCTAGAA AGCAAGGCAG CACGACAGAC 
AATGTCAGTC ACCTGTTTGC TGAGATGGAC CCAGATCAGC CGGCCACCGC CATCGTCAAC 
TTTGTCTCTA AAGTGATGAT CAGCTCTCAG AAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303244.2
CDS	715..6033
Translation	MPSVSLSLPAALAGRARTWVCLSCMFWPEEFEAVHSHTFRLKTFKKGKHCGVCKQTVNNEGLICRVCRLACHRKCEVKVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSESEDEEEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAGQKSSADGHACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_009304969.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304969.2

ORF Insert Sequence:

ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG 
CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG 
GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG 
TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA 
CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG 
TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC 
ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC 
GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC 
TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT 
GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG 
GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA 
GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC 
GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG 
GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT 
AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC 
GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC 
ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA 
CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC 
CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT 
ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT 
GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT 
GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT 
TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA 
GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG 
GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT 
CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC 
ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT 
TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA 
GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT 
GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA 
GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT 
CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA 
GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG 
CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG 
TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA 
GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG 
AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC 
TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA 
GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT 
ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA 
CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC 
CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT 
TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT 
TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA 
GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG 
CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA 
CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA 
TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG 
GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC 
ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG 
CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT 
CCAATTGTTG GTGGTCGATC TCCTCCAGGA TTGGCTAAAA CACCACTGTC CTTTCTGGGC 
CTGAAGCCCC ACAACCCTGC TGAGATCCTG CTCAACCAGA CAGGCTCAGA GAGTGAGGAT 
GAGGAAGAGC CTCGTAGTTA TGTGGAGTCT GTGGCGAAGG CAGCAGCAGT ACCTGCATCT 
CCAACATATG ACAGCGACAG CTTAGCTCGT CCTGCCGGAC CGTCCCATGC TTACAATGCT 
CCACTTTCCT CCAGCAGCCC CATTCAGAAC CCAGATCCAG TTGAAAGCAC ATTGGCTGAC 
AGCGGGGTCG GGATGAATTC TTTATCCCAG GCTGCTGTCG ACATTTTCCA TTTCCAGCCC 
TCCGAAATCC CATACCAACC ACCACCACCC CCCTACCCAA CCTCCATGTT GGGTAGCTAC 
ATCAGCCCTT CTCCAGCAGA GGCCATGCAA AATAATACCA CCACCATCCA GCCACTTTCT 
GGAAGTCCTG GGACAAGACA CCACACCATC CTTTCTGACA TGGCGTCCGT TCCCAACTTT 
CAACACAGAT TAGCAAATCA GGATGGTCCC ATACTTGGGC GACAGCAGGC TCCAGCTAAT 
GGTCATGTGC CTGGAATTGC TGGTAGTCCA GTGCAAGGAA GACACACTAT GGTGTCCCAG 
GGTACACAAA GCAGTCCCAT ACTGAGCAGA CAGTATCCTG TCACCCAGGG GATTCAGAGT 
AGTCCAATTC TCAGCAGGCA GCCAATGGGA CAAGCTGTCC AGAGCAGTCC AGTCCTCAAC 
CGACAGGCAT CCCTGAATCA AGCAAACCAA AGCAGTCCAG TGTTGAGTAG ACAGCAGTCC 
ATTACCCTAC CCAACCAAGG CAGCCCGGTC CTAAGCAGAC AGTCCTCGCT TAGTCATCCC 
AGTCAGGGTA GTCCTGTACT AGGACGCCAC CCTTCCCTAA CACAGGGTAG CCCCAGCCTG 
GATAGGCATC CGATGTACAG CGGTTACACC ACTCCAGAAG AGCGACACGG AGCTCTGTCA 
CGCCAAAGCA GCTCTTCAGG ATACCAGCCG CCATCTACAC CGTCTTTCCC AGTTTCTCCA 
GCAGGATACA TCGAGGGAGC GGGATTCAGA CAGGGGAGTC CTGCTTTCCA ACCTCAGTTG 
CCCGAAAAGA GACGCATGTC CAGCAGTGAC CGCACCAATG GTGGCCTGTC CTATGGGACG 
CTCAATGGGA AGATGTCCTC ACCGGTGTCC AGTGGCGGCA GCACTCCGAG TGTTCATTTC 
TTCCACACAC TCCCCGATTT CTCCAAACTC AACATGTGTG ATGGAAGTCC AGAAACCAGA 
ATGAACGTGA AGTTTGTCCA GGACACGTCC AAATTCTGGT ATAAACCAGA TATCTCGAGA 
GACCAAGCGA TTAACATGCT GAAGGATCAA GATCCTGGGG CCTTTGTGAT TCGGGACAGT 
CATTCATTCA GAGGAGCCTA CGGCTTGGCT ATGAAAGTGG CTTCACCACC TCCAACCACT 
CAACCTACAA AGAAAGCTGG AGATGTCACT AATGAGTTGG TGAGGCATTT TCTAATTGAG 
ACGAGTCCGA AGGGTGTGAG ACTGAAGGGC TGCCCCAACG AGCCGTACTT TGGCTGCCTG 
TCTGCGCTGG TCTACCAGCA CTCCATCACT CCACTGGCAC TGCCCTGCAA ACTAGTCATA 
CCAACCAGAG ATCCTCTGGA AGAGTCACCT GAGATCGCAA CACCAACCAA CCCGGCGGCA 
GATATGCTCA AGCAGGGCGC AGGGCAGAAG AGTTCAGCTG ATGGCCATGC ATGCAATGTG 
CTGTACATCA ACTCTGTGGA TATGGAGTCT TTGACTGGAC CTCAAGCCAT CGCCAAAGCC 
ATTTCAGAAA CCATGAACGC CAGTCCGGCA CCCTCAGCCA CCATTGTTCA CTTCAAGGTG 
TCGGCACAAG GCATCACACT CACAGACAAC CAGAGAAAGC TTTTCTTCAG ACGTCATTAT 
CCCATCGGCA CAGTCACGTT CTGTGACATT GATCCACAGG AGAGGAAGTG GAACAAACCA 
GAGGGTGGCA CTGCCAAATT CTTTGGCTTT GTGGCTAGAA AGCAAGGCAG CACGACAGAC 
AATGTCAGTC ACCTGTTTGC TGAGATGGAC CCAGATCAGC CGGCCACCGC CATCGTCAAC 
TTTGTCTCTA AAGTGATGAT CAGCTCTCAG AAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_005167931.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167988.2 Danio rerio tensin 1b (tns1b), transcript variant X6, mRNA.

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CloneID	ODa60380
Clone ID Related Accession (Same CDS sequence)	XM_005167931.3
Accession Version	XM_005167931.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tensin-1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167931.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG 
CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG 
GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG 
TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA 
CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG 
TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC 
ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC 
GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC 
TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT 
GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG 
GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA 
GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC 
GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG 
GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT 
AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC 
GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC 
ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA 
CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC 
CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT 
ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT 
GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT 
GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT 
TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA 
GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG 
GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT 
CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC 
ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT 
TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA 
GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT 
GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA 
GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT 
CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA 
GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG 
CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG 
TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA 
GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG 
AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC 
TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA 
GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT 
ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA 
CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC 
CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT 
TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT 
TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA 
GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG 
CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA 
CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA 
TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG 
GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC 
ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG 
CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT 
CCAATTGTTG GTGGTCGATC TCCTCCAGGA TTGGCTAAAA CACCACTGTC CTTTCTGGGC 
CTGAAGCCCC ACAACCCTGC TGAGATCCTG CTCAACCAGA CAGGCTCAGA GCCTCGTAGT 
TATGTGGAGT CTGTGGCGAA GGCAGCAGCA GTACCTGCAT CTCCAACATA TGACAGCGAC 
AGCTTAGCTC GTCCTGCCGG ACCGTCCCAT GCTTACAATG CTCCACTTTC CTCCAGCAGC 
CCCATTCAGA ACCCAGATCC AGTTGAAAGC ACATTGGCTG ACAGCGGGGT CGGGATGAAT 
TCTTTATCCC AGGCTGCTGT CGACATTTTC CATTTCCAGC CCTCCGAAAT CCCATACCAA 
CCACCACCAC CCCCCTACCC AACCTCCATG TTGGGTAGCT ACATCAGCCC TTCTCCAGCA 
GAGGCCATGC AAAATAATAC CACCACCATC CAGCCACTTT CTGGAAGTCC TGGGACAAGA 
CACCACACCA TCCTTTCTGA CATGGCGTCC GTTCCCAACT TTCAACACAG ATTAGCAAAT 
CAGGATGGTC CCATACTTGG GCGACAGCAG GCTCCAGCTA ATGGTCATGT GCCTGGAATT 
GCTGGTAGTC CAGTGCAAGG AAGACACACT ATGGTGTCCC AGGGTACACA AAGCAGTCCC 
ATACTGAGCA GACAGTATCC TGTCACCCAG GGGATTCAGA GTAGTCCAAT TCTCAGCAGG 
CAGCCAATGG GACAAGCTGT CCAGAGCAGT CCAGTCCTCA ACCGACAGGC ATCCCTGAAT 
CAAGCAAACC AAAGCAGTCC AGTGTTGAGT AGACAGCAGT CCATTACCCT ACCCAACCAA 
GGCAGCCCGG TCCTAAGCAG ACAGTCCTCG CTTAGTCATC CCAGTCAGGG TAGTCCTGTA 
CTAGGACGCC ACCCTTCCCT AACACAGGGT AGCCCCAGCC TGGATAGGCA TCCGATGTAC 
AGCGGTTACA CCACTCCAGA AGAGCGACAC GGAGCTCTGT CACGCCAAAG CAGCTCTTCA 
GGATACCAGC CGCCATCTAC ACCGTCTTTC CCAGTTTCTC CAGCAGGATA CATCGAGGGA 
GCGGGATTCA GACAGGGGAG TCCTGCTTTC CAACCTCAGT TGCCCGAAAA GAGACGCATG 
TCCAGCAGTG ACCGCACCAA TGGTGGCCTG TCCTATGGGA CGCTCAATGG GAAGATGTCC 
TCACCGGTGT CCAGTGGCGG CAGCACTCCG AGTGTTCATT TCTTCCACAC ACTCCCCGAT 
TTCTCCAAAC TCAACATGTG TGATGGAAGT CCAGAAACCA GAATGAACGT GAAGTTTGTC 
CAGGACACGT CCAAATTCTG GTATAAACCA GATATCTCGA GAGACCAAGC GATTAACATG 
CTGAAGGATC AAGATCCTGG GGCCTTTGTG ATTCGGGACA GTCATTCATT CAGAGGAGCC 
TACGGCTTGG CTATGAAAGT GGCTTCACCA CCTCCAACCA CTCAACCTAC AAAGAAAGCT 
GGAGATGTCA CTAATGAGTT GGTGAGGCAT TTTCTAATTG AGACGAGTCC GAAGGGTGTG 
AGACTGAAGG GCTGCCCCAA CGAGCCGTAC TTTGGCTGCC TGTCTGCGCT GGTCTACCAG 
CACTCCATCA CTCCACTGGC ACTGCCCTGC AAACTAGTCA TACCAACCAG AGATCCTCTG 
GAAGAGTCAC CTGAGATCGC AACACCAACC AACCCGGCGG CAGATATGCT CAAGCAGGGC 
GCAGGGCAGA AGAGTTCAGC TGATGGCCAT GCATGCAATG TGCTGTACAT CAACTCTGTG 
GATATGGAGT CTTTGACTGG ACCTCAAGCC ATCGCCAAAG CCATTTCAGA AACCATGAAC 
GCCAGTCCGG CACCCTCAGC CACCATTGTT CACTTCAAGG TGTCGGCACA AGGCATCACA 
CTCACAGACA ACCAGAGAAA GCTTTTCTTC AGACGTCATT ATCCCATCGG CACAGTCACG 
TTCTGTGACA TTGATCCACA GGAGAGGAAG TGGAACAAAC CAGAGGGTGG CACTGCCAAA 
TTCTTTGGCT TTGTGGCTAG AAAGCAAGGC AGCACGACAG ACAATGTCAG TCACCTGTTT 
GCTGAGATGG ACCCAGATCA GCCGGCCACC GCCATCGTCA ACTTTGTCTC TAAAGTGATG 
ATCAGCTCTC AGAAGCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167988.2
CDS	714..6014
Translation	MPSVSLSLPAALAGRARTWVCLSCMFWPEEFEAVHSHTFRLKTFKKGKHCGVCKQTVNNEGLICRVCRLACHRKCEVKVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAGQKSSADGHACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_005167931.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167931.3

ORF Insert Sequence:

ATGCCCTCCG TGTCTCTCAG TCTGCCCGCC GCTCTGGCCG GCCGCGCTCG TACCTGGGTC 
TGCCTGTCCT GCATGTTCTG GCCTGAAGAG TTTGAAGCCG TTCATTCGCA CACCTTCCGG 
CTGAAGACGT TCAAGAAGGG GAAACACTGT GGTGTTTGCA AGCAGACGGT CAACAATGAG 
GGGCTCATCT GCAGAGTTTG CCGGTTAGCC TGTCATCGGA AGTGTGAAGT CAAGGTCTCG 
TCCTCCTGTG TTCCTGTCGC CAACTATGAA CTGGCCCCCA GCAATGAGCT CCCCCTGAAA 
CATATCGAGA CCACAGGCTC AACCAAATCC TCCAAGAGCA TGGAGTCTCG ACGCAGACCG 
TCAAGGAGTT TGAGTTTGAT CCAGGCCATG GAGGAGAACT ATGAGGTGGA TCTGGTTTAC 
ATCACCGAGC GCATCATTTC TGTCACCTTT CCCAGTAATG CAGAGGAGCC CAGTTACAGC 
GCCAACATTA AAGAAGTGGC AAACATGCTG CGCTCCAAAC ATGGAGACAA CTACCTGCTC 
TTCAACCTCA GTGAAAAGCG CTTGGATATC TCCAAGCTGA ATCCCAAAGT TCTGGATTTT 
GGCTGGCCGG ATCATCACGC TCCTGCTTTG GATAAGATCT GCAGCATCTG CAAAGCCATG 
GACACTTGGA TGAACGCTGG CAGTCACAAC GTGGTGGTCA TACACAACAA GGGAAACCGA 
GGTCGAACTG GGGTGGTTGT GGCTGCGTAC ATGCATTACA GCAACATATC AGCGAGTGCC 
GACCAGGCTC TGGACAGGTT TGCCATGAAG AGGTTTTATG AAGATAAAGT GCTTCCTGTG 
GGTCAGCCTT CCCAGAGAAG ATATGTGCAG TACTTCAGCG GTCTGCTCTC CGGCCACATT 
AAGATCAACA ACAAACCCCT GTTTCTCCAT CACGTCATCA TGCACGGCAT CCCAAACTTC 
GAGTCTAAAG GAGGTTGCCG CCCTTTCCTG AAGATCTACC AGGCCATGCA GCCCGTCTAC 
ACATCTGGCA TCTATAATGT CCAAGGAGAC AGTCAGACTA GTATCTGCAT CACCATTGAA 
CCGGGCCTGC TTTTGAAAGG AGACATACTG CTAAAGTGTT ATCACAAGCG TTTCCGCAGC 
CCATGCCGTG ATGTCATATT CAGAGTTCAG TTTCACACCT GTGCTGTTCA TGATCTGGGT 
ATCGTCTTCG GCAAAGATGA GCTTGATGAA ACCTTTAAAG ACGACAGATT TCCAGAATAT 
GGAAAGGTGG AATTCGTCTT TTCTTTTGGC CCAGAAAAAA TCCAAGGAAT GGACCACCTT 
GAGAACGGGC CCAGTGTTTC TGTGGACTAC AATACCCAAG ATGCACTAAT CCGCTGGGAT 
TCCTACGAGA ACTTCAACCA GCGCTGTGAA GACTCCATGG AAGACGTTAT TCACACCGAA 
GGTCCATTGG ATGGTAGTCT CTACGCTAAA GTTCGTAAGA AAGAGTCTCT GGAAGGAGGG 
GTTACAGCCA ATGGTCTCCC AGCTGCTGCT GATCACACTT TACCTGCTGT AGACCACACT 
CTACCACCAG TTGACCACGC CTTGTCTGTA AGCAGTGATT CAGGCAACTC CACAGCGTCC 
ATCAAGACTG ACCGCACTGA TGAAGCCAGC CATCAGCCAG CATTGGTGAG TCAGCCATCT 
TCCCAGCCCC TCAGCCCTGA GGAGAAACAG GAGCTGGACC AGTTGCTCAG TGGTTTGGAA 
GCACCAATGC ATCGCACAGG TTACCAAAGC AGCCCTGGTG GGGGTGGTGG TGGAGGTGGT 
GGAGTCCTAC ATCTCGTCCC TGCCCAGGTA CACGTCAATG GACACAGCAA CATAGACAGA 
GAGACAGATA TCCTTGATGA TGACTTGCCC ATCAGCCAGG AAGGGAACAG TGTGGACAGT 
CTTGGAACTC TCTCATCTTT TGAGGGCAGA GCCACTCCTG CGGATCTATA CTACCAGTCA 
GAGACAGTCA TTAATGGACA AGATATGCCC TACTTAGAGA AGAGTGTCCA TGAGAAAATG 
CTAGAGCCCA GAGTTCACAG CTCATCATCT GTCCAAGAGC ACTTGGGAGC CCCTAGTGGG 
TATGCTAACC AGAATGGCAA CATCTATCGA TCTCAGTCAT TAGGAGCAAC CCCGAACACA 
GACCAGAAGT ACATGCCCAA AGCTCCAGCA CGCACAACCA GCAGTAGGGA TGCCGTGCAG 
AGACTGAATG TTTGGCAACA ATTTGGTGTA CCAGAAGTCC CAGTCACAGA TGGGGTCACC 
TTCAACCCTG TACTACAATC CCACAGCCTT CCAGAGTTCC CTCAGATGGC ATCTCAACAA 
GAGATTGAGC AGTCCATTGA AGCCCTCAAC ATGCTGATGC TGGACCTTGA TCCAGCACTT 
ACTCAAGTCC CCAAATCCCA CAGCGCTCCA TCTGGAGAAA GTGGTGTGGT CACCACCCAA 
CCATCTTTCT CTCAAACCCA AGCACGCCCA TCCTACCAGG CTGACACTGC TATTCATGAC 
CGCGGTGCGG CCTCTTCAAT GGGATTTCCT TTGACCTTTC AACAAGTAAC TGGAGAGCCT 
TCACAACCCA CCATGAAACA GCAGAAACCC ACAACTACAT CCCAGCATGT CGAATTCGCT 
TCTGAAGCAG GAGGTCAGGC CCCCATGTCC CAGTCCACAG TGCCTTCCTC CACCTTTGGA 
GTTCTCCAGT TGAAACCCCT CAACATTTAT CCCTCAAGTA CCACATCCCA GTCCCCCGAG 
CCTCAGGAGA CTCAGCGTAG CTACTCTTCT TCCTCATCCC CTCTTCCCAA AGAGTCTGAA 
CCAGAAGATA GCAGCTACAA ATTGGAGGGT CTTGTAGCTC ACCGTGTAGC TGGACTTCCA 
TCTGGTGAAA TGTCGCCAGA TGAGGCTGCA GTTCCTGGAC GCTGTCGGAC CCTTAGTGAG 
GGCCTTTCCC AAGACGAAAG CCCAACTCGA GGAAGCTCAG TCCGCTCCCC CATTCGCTGC 
ATCTCACCAG AGCTGGCCAA TACAATTGCT CTGAACCCTG GGGGGCGGCC CAAGGAGAGG 
CACATGCACA GCTACAGAGA AGCGTTTGAG GAAATGGATG GGGGTCCCGT TAGCCCCCCT 
CCAATTGTTG GTGGTCGATC TCCTCCAGGA TTGGCTAAAA CACCACTGTC CTTTCTGGGC 
CTGAAGCCCC ACAACCCTGC TGAGATCCTG CTCAACCAGA CAGGCTCAGA GCCTCGTAGT 
TATGTGGAGT CTGTGGCGAA GGCAGCAGCA GTACCTGCAT CTCCAACATA TGACAGCGAC 
AGCTTAGCTC GTCCTGCCGG ACCGTCCCAT GCTTACAATG CTCCACTTTC CTCCAGCAGC 
CCCATTCAGA ACCCAGATCC AGTTGAAAGC ACATTGGCTG ACAGCGGGGT CGGGATGAAT 
TCTTTATCCC AGGCTGCTGT CGACATTTTC CATTTCCAGC CCTCCGAAAT CCCATACCAA 
CCACCACCAC CCCCCTACCC AACCTCCATG TTGGGTAGCT ACATCAGCCC TTCTCCAGCA 
GAGGCCATGC AAAATAATAC CACCACCATC CAGCCACTTT CTGGAAGTCC TGGGACAAGA 
CACCACACCA TCCTTTCTGA CATGGCGTCC GTTCCCAACT TTCAACACAG ATTAGCAAAT 
CAGGATGGTC CCATACTTGG GCGACAGCAG GCTCCAGCTA ATGGTCATGT GCCTGGAATT 
GCTGGTAGTC CAGTGCAAGG AAGACACACT ATGGTGTCCC AGGGTACACA AAGCAGTCCC 
ATACTGAGCA GACAGTATCC TGTCACCCAG GGGATTCAGA GTAGTCCAAT TCTCAGCAGG 
CAGCCAATGG GACAAGCTGT CCAGAGCAGT CCAGTCCTCA ACCGACAGGC ATCCCTGAAT 
CAAGCAAACC AAAGCAGTCC AGTGTTGAGT AGACAGCAGT CCATTACCCT ACCCAACCAA 
GGCAGCCCGG TCCTAAGCAG ACAGTCCTCG CTTAGTCATC CCAGTCAGGG TAGTCCTGTA 
CTAGGACGCC ACCCTTCCCT AACACAGGGT AGCCCCAGCC TGGATAGGCA TCCGATGTAC 
AGCGGTTACA CCACTCCAGA AGAGCGACAC GGAGCTCTGT CACGCCAAAG CAGCTCTTCA 
GGATACCAGC CGCCATCTAC ACCGTCTTTC CCAGTTTCTC CAGCAGGATA CATCGAGGGA 
GCGGGATTCA GACAGGGGAG TCCTGCTTTC CAACCTCAGT TGCCCGAAAA GAGACGCATG 
TCCAGCAGTG ACCGCACCAA TGGTGGCCTG TCCTATGGGA CGCTCAATGG GAAGATGTCC 
TCACCGGTGT CCAGTGGCGG CAGCACTCCG AGTGTTCATT TCTTCCACAC ACTCCCCGAT 
TTCTCCAAAC TCAACATGTG TGATGGAAGT CCAGAAACCA GAATGAACGT GAAGTTTGTC 
CAGGACACGT CCAAATTCTG GTATAAACCA GATATCTCGA GAGACCAAGC GATTAACATG 
CTGAAGGATC AAGATCCTGG GGCCTTTGTG ATTCGGGACA GTCATTCATT CAGAGGAGCC 
TACGGCTTGG CTATGAAAGT GGCTTCACCA CCTCCAACCA CTCAACCTAC AAAGAAAGCT 
GGAGATGTCA CTAATGAGTT GGTGAGGCAT TTTCTAATTG AGACGAGTCC GAAGGGTGTG 
AGACTGAAGG GCTGCCCCAA CGAGCCGTAC TTTGGCTGCC TGTCTGCGCT GGTCTACCAG 
CACTCCATCA CTCCACTGGC ACTGCCCTGC AAACTAGTCA TACCAACCAG AGATCCTCTG 
GAAGAGTCAC CTGAGATCGC AACACCAACC AACCCGGCGG CAGATATGCT CAAGCAGGGC 
GCAGGGCAGA AGAGTTCAGC TGATGGCCAT GCATGCAATG TGCTGTACAT CAACTCTGTG 
GATATGGAGT CTTTGACTGG ACCTCAAGCC ATCGCCAAAG CCATTTCAGA AACCATGAAC 
GCCAGTCCGG CACCCTCAGC CACCATTGTT CACTTCAAGG TGTCGGCACA AGGCATCACA 
CTCACAGACA ACCAGAGAAA GCTTTTCTTC AGACGTCATT ATCCCATCGG CACAGTCACG 
TTCTGTGACA TTGATCCACA GGAGAGGAAG TGGAACAAAC CAGAGGGTGG CACTGCCAAA 
TTCTTTGGCT TTGTGGCTAG AAAGCAAGGC AGCACGACAG ACAATGTCAG TCACCTGTTT 
GCTGAGATGG ACCCAGATCA GCCGGCCACC GCCATCGTCA ACTTTGTCTC TAAAGTGATG 
ATCAGCTCTC AGAAGCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_017357850.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213339.1 Danio rerio tensin 1b (tns1b), transcript variant X7, mRNA.

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CloneID	ODa60381
Clone ID Related Accession (Same CDS sequence)	XM_017357850.1
Accession Version	XM_017357850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tensin-1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCG CTGTGTTTCA GGTCTCGTCC TCCTGTGTTC CTGTCGCCAA CTATGAACTG 
GCCCCCAGCA ATGAGCTCCC CCTGAAACAT ATCGAGACCA CAGGCTCAAC CAAATCCTCC 
AAGAGCATGG AGTCTCGACG CAGACCGTCA AGGAGTTTGA GTTTGATCCA GGCCATGGAG 
GAGAACTATG AGGTGGATCT GGTTTACATC ACCGAGCGCA TCATTTCTGT CACCTTTCCC 
AGTAATGCAG AGGAGCCCAG TTACAGCGCC AACATTAAAG AAGTGGCAAA CATGCTGCGC 
TCCAAACATG GAGACAACTA CCTGCTCTTC AACCTCAGTG AAAAGCGCTT GGATATCTCC 
AAGCTGAATC CCAAAGTTCT GGATTTTGGC TGGCCGGATC ATCACGCTCC TGCTTTGGAT 
AAGATCTGCA GCATCTGCAA AGCCATGGAC ACTTGGATGA ACGCTGGCAG TCACAACGTG 
GTGGTCATAC ACAACAAGGG AAACCGAGGT CGAACTGGGG TGGTTGTGGC TGCGTACATG 
CATTACAGCA ACATATCAGC GAGTGCCGAC CAGGCTCTGG ACAGGTTTGC CATGAAGAGG 
TTTTATGAAG ATAAAGTGCT TCCTGTGGGT CAGCCTTCCC AGAGAAGATA TGTGCAGTAC 
TTCAGCGGTC TGCTCTCCGG CCACATTAAG ATCAACAACA AACCCCTGTT TCTCCATCAC 
GTCATCATGC ACGGCATCCC AAACTTCGAG TCTAAAGGAG GTTGCCGCCC TTTCCTGAAG 
ATCTACCAGG CCATGCAGCC CGTCTACACA TCTGGCATCT ATAATGTCCA AGGAGACAGT 
CAGACTAGTA TCTGCATCAC CATTGAACCG GGCCTGCTTT TGAAAGGAGA CATACTGCTA 
AAGTGTTATC ACAAGCGTTT CCGCAGCCCA TGCCGTGATG TCATATTCAG AGTTCAGTTT 
CACACCTGTG CTGTTCATGA TCTGGGTATC GTCTTCGGCA AAGATGAGCT TGATGAAACC 
TTTAAAGACG ACAGATTTCC AGAATATGGA AAGGTGGAAT TCGTCTTTTC TTTTGGCCCA 
GAAAAAATCC AAGGAATGGA CCACCTTGAG AACGGGCCCA GTGTTTCTGT GGACTACAAT 
ACCCAAGATG CACTAATCCG CTGGGATTCC TACGAGAACT TCAACCAGCG CTGTGAAGAC 
TCCATGGAAG ACGTTATTCA CACCGAAGGT CCATTGGATG GTAGTCTCTA CGCTAAAGTT 
CGTAAGAAAG AGTCTCTGGA AGGAGGGGTT ACAGCCAATG GTCTCCCAGC TGCTGCTGAT 
CACACTTTAC CTGCTGTAGA CCACACTCTA CCACCAGTTG ACCACGCCTT GTCTGTAAGC 
AGTGATTCAG GCAACTCCAC AGCGTCCATC AAGACTGACC GCACTGATGA AGCCAGCCAT 
CAGCCAGCAT TGGTGAGTCA GCCATCTTCC CAGCCCCTCA GCCCTGAGGA GAAACAGGAG 
CTGGACCAGT TGCTCAGTGG TTTGGAAGCA CCAATGCATC GCACAGGTTA CCAAAGCAGC 
CCTGGTGGGG GTGGTGGTGG AGGTGGTGGA GTCCTACATC TCGTCCCTGC CCAGGTACAC 
GTCAATGGAC ACAGCAACAT AGACAGAGAG ACAGATATCC TTGATGATGA CTTGCCCATC 
AGCCAGGAAG GGAACAGTGT GGACAGTCTT GGAACTCTCT CATCTTTTGA GGGCAGAGCC 
ACTCCTGCGG ATCTATACTA CCAGTCAGAG ACAGTCATTA ATGGACAAGA TATGCCCTAC 
TTAGAGAAGA GTGTCCATGA GAAAATGCTA GAGCCCAGAG TTCACAGCTC ATCATCTGTC 
CAAGAGCACT TGGGAGCCCC TAGTGGGTAT GCTAACCAGA ATGGCAACAT CTATCGATCT 
CAGTCATTAG GAGCAACCCC GAACACAGAC CAGAAGTACA TGCCCAAAGC TCCAGCACGC 
ACAACCAGCA GTAGGGATGC CGTGCAGAGA CTGAATGTTT GGCAACAATT TGGTGTACCA 
GAAGTCCCAG TCACAGATGG GGTCACCTTC AACCCTGTAC TACAATCCCA CAGCCTTCCA 
GAGTTCCCTC AGATGGCATC TCAACAAGAG ATTGAGCAGT CCATTGAAGC CCTCAACATG 
CTGATGCTGG ACCTTGATCC AGCACTTACT CAAGTCCCCA AATCCCACAG CGCTCCATCT 
GGAGAAAGTG GTGTGGTCAC CACCCAACCA TCTTTCTCTC AAACCCAAGC ACGCCCATCC 
TACCAGGCTG ACACTGCTAT TCATGACCGC GGTGCGGCCT CTTCAATGGG ATTTCCTTTG 
ACCTTTCAAC AAGTAACTGG AGAGCCTTCA CAACCCACCA TGAAACAGCA GAAACCCACA 
ACTACATCCC AGCATGTCGA ATTCGCTTCT GAAGCAGGAG GTCAGGCCCC CATGTCCCAG 
TCCACAGTGC CTTCCTCCAC CTTTGGAGTT CTCCAGTTGA AACCCCTCAA CATTTATCCC 
TCAAGTACCA CATCCCAGTC CCCCGAGCCT CAGGAGACTC AGCGTAGCTA CTCTTCTTCC 
TCATCCCCTC TTCCCAAAGA GTCTGAACCA GAAGATAGCA GCTACAAATT GGAGGGTCTT 
GTAGCTCACC GTGTAGCTGG ACTTCCATCT GGTGAAATGT CGCCAGATGA GGCTGCAGTT 
CCTGGACGCT GTCGGACCCT TAGTGAGGGC CTTTCCCAAG ACGAAAGCCC AACTCGAGGA 
AGCTCAGTCC GCTCCCCCAT TCGCTGCATC TCACCAGAGC TGGCCAATAC AATTGCTCTG 
AACCCTGGGG GGCGGCCCAA GGAGAGGCAC ATGCACAGCT ACAGAGAAGC GTTTGAGGAA 
ATGGATGGGG GTCCCGTTAG CCCCCCTCCA ATTGTTGGTG GTGAGGTCCT ACCCCAAACC 
CCTGCCTTCC CTGTCTCGCC ACAAACCCCT TACTTCAACA TGTGTCGATC TCCTCCAGGA 
TTGGCTAAAA CACCACTGTC CTTTCTGGGC CTGAAGCCCC ACAACCCTGC TGAGATCCTG 
CTCAACCAGA CAGGCTCAGA GAGTGAGGAT GAGGAAGAGC CTCGTAGTTA TGTGGAGTCT 
GTGGCGAAGG CAGCAGCAGT ACCTGCATCT CCAACATATG ACAGCGACAG CTTAGCTCGT 
CCTGCCGGAC CGTCCCATGC TTACAATGCT CCACTTTCCT CCAGCAGCCC CATTCAGAAC 
CCAGATCCAG TTGAAAGCAC ATTGGCTGAC AGCGGGGTCG GGATGAATTC TTTATCCCAG 
GCTGCTGTCG ACATTTTCCA TTTCCAGCCC TCCGAAATCC CATACCAACC ACCACCACCC 
CCCTACCCAA CCTCCATGTT GGGTAGCTAC ATCAGCCCTT CTCCAGCAGA GGCCATGCAA 
AATAATACCA CCACCATCCA GCCACTTTCT GGAAGTCCTG GGACAAGACA CCACACCATC 
CTTTCTGACA TGGCGTCCGT TCCCAACTTT CAACACAGAT TAGCAAATCA GGATGGTCCC 
ATACTTGGGC GACAGCAGGC TCCAGCTAAT GGTCATGTGC CTGGAATTGC TGGTAGTCCA 
GTGCAAGGAA GACACACTAT GGTGTCCCAG GGTACACAAA GCAGTCCCAT ACTGAGCAGA 
CAGTATCCTG TCACCCAGGG GATTCAGAGT AGTCCAATTC TCAGCAGGCA GCCAATGGGA 
CAAGCTGTCC AGAGCAGTCC AGTCCTCAAC CGACAGGCAT CCCTGAATCA AGCAAACCAA 
AGCAGTCCAG TGTTGAGTAG ACAGCAGTCC ATTACCCTAC CCAACCAAGG CAGCCCGGTC 
CTAAGCAGAC AGTCCTCGCT TAGTCATCCC AGTCAGGGTA GTCCTGTACT AGGACGCCAC 
CCTTCCCTAA CACAGGGTAG CCCCAGCCTG GATAGGCATC CGATGTACAG CGGTTACACC 
ACTCCAGAAG AGCGACACGG AGCTCTGTCA CGCCAAAGCA GCTCTTCAGG ATACCAGCCG 
CCATCTACAC CGTCTTTCCC AGTTTCTCCA GCAGGATACA TCGAGGGAGC GGGATTCAGA 
CAGGGGAGTC CTGCTTTCCA ACCTCAGTTG CCCGAAAAGA GACGCATGTC CAGCAGTGAC 
CGCACCAATG GTGGCCTGTC CTATGGGACG CTCAATGGGA AGATGTCCTC ACCGGTGTCC 
AGTGGCGGCA GCACTCCGAG TGTTCATTTC TTCCACACAC TCCCCGATTT CTCCAAACTC 
AACATGTGTG ATGGAAGTCC AGAAACCAGA ATGAACGTGA AGTTTGTCCA GGACACGTCC 
AAATTCTGGT ATAAACCAGA TATCTCGAGA GACCAAGCGA TTAACATGCT GAAGGATCAA 
GATCCTGGGG CCTTTGTGAT TCGGGACAGT CATTCATTCA GAGGAGCCTA CGGCTTGGCT 
ATGAAAGTGG CTTCACCACC TCCAACCACT CAACCTACAA AGAAAGCTGG AGATGTCACT 
AATGAGTTGG TGAGGCATTT TCTAATTGAG ACGAGTCCGA AGGGTGTGAG ACTGAAGGGC 
TGCCCCAACG AGCCGTACTT TGGCTGCCTG TCTGCGCTGG TCTACCAGCA CTCCATCACT 
CCACTGGCAC TGCCCTGCAA ACTAGTCATA CCAACCAGAG ATCCTCTGGA AGAGTCACCT 
GAGATCGCAA CACCAACCAA CCCGGCGGCA GATATGCTCA AGCAGGGCGC AGGGCAGAAG 
AGTTCAGCTG ATGGCCATGC ATGCAATGTG CTGTACATCA ACTCTGTGGA TATGGAGTCT 
TTGACTGGAC CTCAAGCCAT CGCCAAAGCC ATTTCAGAAA CCATGAACGC CAGTCCGGCA 
CCCTCAGCCA CCATTGTTCA CTTCAAGGTG TCGGCACAAG GCATCACACT CACAGACAAC 
CAGAGAAAGC TTTTCTTCAG ACGTCATTAT CCCATCGGCA CAGTCACGTT CTGTGACATT 
GATCCACAGG AGAGGAAGTG GAACAAACCA GAGGGTGGCA CTGCCAAATT CTTTGGCTTT 
GTGGCTAGAA AGCAAGGCAG CACGACAGAC AATGTCAGTC ACCTGTTTGC TGAGATGGAC 
CCAGATCAGC CGGCCACCGC CATCGTCAAC TTTGTCTCTA AAGTGATGAT CAGCTCTCAG 
AAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213339.1
CDS	387..5555
Translation	MATAVFQVSSSCVPVANYELAPSNELPLKHIETTGSTKSSKSMESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGEVLPQTPAFPVSPQTPYFNMCRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSESEDEEEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAGQKSSADGHACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_017357850.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357850.1

ORF Insert Sequence:

ATGGCCACCG CTGTGTTTCA GGTCTCGTCC TCCTGTGTTC CTGTCGCCAA CTATGAACTG 
GCCCCCAGCA ATGAGCTCCC CCTGAAACAT ATCGAGACCA CAGGCTCAAC CAAATCCTCC 
AAGAGCATGG AGTCTCGACG CAGACCGTCA AGGAGTTTGA GTTTGATCCA GGCCATGGAG 
GAGAACTATG AGGTGGATCT GGTTTACATC ACCGAGCGCA TCATTTCTGT CACCTTTCCC 
AGTAATGCAG AGGAGCCCAG TTACAGCGCC AACATTAAAG AAGTGGCAAA CATGCTGCGC 
TCCAAACATG GAGACAACTA CCTGCTCTTC AACCTCAGTG AAAAGCGCTT GGATATCTCC 
AAGCTGAATC CCAAAGTTCT GGATTTTGGC TGGCCGGATC ATCACGCTCC TGCTTTGGAT 
AAGATCTGCA GCATCTGCAA AGCCATGGAC ACTTGGATGA ACGCTGGCAG TCACAACGTG 
GTGGTCATAC ACAACAAGGG AAACCGAGGT CGAACTGGGG TGGTTGTGGC TGCGTACATG 
CATTACAGCA ACATATCAGC GAGTGCCGAC CAGGCTCTGG ACAGGTTTGC CATGAAGAGG 
TTTTATGAAG ATAAAGTGCT TCCTGTGGGT CAGCCTTCCC AGAGAAGATA TGTGCAGTAC 
TTCAGCGGTC TGCTCTCCGG CCACATTAAG ATCAACAACA AACCCCTGTT TCTCCATCAC 
GTCATCATGC ACGGCATCCC AAACTTCGAG TCTAAAGGAG GTTGCCGCCC TTTCCTGAAG 
ATCTACCAGG CCATGCAGCC CGTCTACACA TCTGGCATCT ATAATGTCCA AGGAGACAGT 
CAGACTAGTA TCTGCATCAC CATTGAACCG GGCCTGCTTT TGAAAGGAGA CATACTGCTA 
AAGTGTTATC ACAAGCGTTT CCGCAGCCCA TGCCGTGATG TCATATTCAG AGTTCAGTTT 
CACACCTGTG CTGTTCATGA TCTGGGTATC GTCTTCGGCA AAGATGAGCT TGATGAAACC 
TTTAAAGACG ACAGATTTCC AGAATATGGA AAGGTGGAAT TCGTCTTTTC TTTTGGCCCA 
GAAAAAATCC AAGGAATGGA CCACCTTGAG AACGGGCCCA GTGTTTCTGT GGACTACAAT 
ACCCAAGATG CACTAATCCG CTGGGATTCC TACGAGAACT TCAACCAGCG CTGTGAAGAC 
TCCATGGAAG ACGTTATTCA CACCGAAGGT CCATTGGATG GTAGTCTCTA CGCTAAAGTT 
CGTAAGAAAG AGTCTCTGGA AGGAGGGGTT ACAGCCAATG GTCTCCCAGC TGCTGCTGAT 
CACACTTTAC CTGCTGTAGA CCACACTCTA CCACCAGTTG ACCACGCCTT GTCTGTAAGC 
AGTGATTCAG GCAACTCCAC AGCGTCCATC AAGACTGACC GCACTGATGA AGCCAGCCAT 
CAGCCAGCAT TGGTGAGTCA GCCATCTTCC CAGCCCCTCA GCCCTGAGGA GAAACAGGAG 
CTGGACCAGT TGCTCAGTGG TTTGGAAGCA CCAATGCATC GCACAGGTTA CCAAAGCAGC 
CCTGGTGGGG GTGGTGGTGG AGGTGGTGGA GTCCTACATC TCGTCCCTGC CCAGGTACAC 
GTCAATGGAC ACAGCAACAT AGACAGAGAG ACAGATATCC TTGATGATGA CTTGCCCATC 
AGCCAGGAAG GGAACAGTGT GGACAGTCTT GGAACTCTCT CATCTTTTGA GGGCAGAGCC 
ACTCCTGCGG ATCTATACTA CCAGTCAGAG ACAGTCATTA ATGGACAAGA TATGCCCTAC 
TTAGAGAAGA GTGTCCATGA GAAAATGCTA GAGCCCAGAG TTCACAGCTC ATCATCTGTC 
CAAGAGCACT TGGGAGCCCC TAGTGGGTAT GCTAACCAGA ATGGCAACAT CTATCGATCT 
CAGTCATTAG GAGCAACCCC GAACACAGAC CAGAAGTACA TGCCCAAAGC TCCAGCACGC 
ACAACCAGCA GTAGGGATGC CGTGCAGAGA CTGAATGTTT GGCAACAATT TGGTGTACCA 
GAAGTCCCAG TCACAGATGG GGTCACCTTC AACCCTGTAC TACAATCCCA CAGCCTTCCA 
GAGTTCCCTC AGATGGCATC TCAACAAGAG ATTGAGCAGT CCATTGAAGC CCTCAACATG 
CTGATGCTGG ACCTTGATCC AGCACTTACT CAAGTCCCCA AATCCCACAG CGCTCCATCT 
GGAGAAAGTG GTGTGGTCAC CACCCAACCA TCTTTCTCTC AAACCCAAGC ACGCCCATCC 
TACCAGGCTG ACACTGCTAT TCATGACCGC GGTGCGGCCT CTTCAATGGG ATTTCCTTTG 
ACCTTTCAAC AAGTAACTGG AGAGCCTTCA CAACCCACCA TGAAACAGCA GAAACCCACA 
ACTACATCCC AGCATGTCGA ATTCGCTTCT GAAGCAGGAG GTCAGGCCCC CATGTCCCAG 
TCCACAGTGC CTTCCTCCAC CTTTGGAGTT CTCCAGTTGA AACCCCTCAA CATTTATCCC 
TCAAGTACCA CATCCCAGTC CCCCGAGCCT CAGGAGACTC AGCGTAGCTA CTCTTCTTCC 
TCATCCCCTC TTCCCAAAGA GTCTGAACCA GAAGATAGCA GCTACAAATT GGAGGGTCTT 
GTAGCTCACC GTGTAGCTGG ACTTCCATCT GGTGAAATGT CGCCAGATGA GGCTGCAGTT 
CCTGGACGCT GTCGGACCCT TAGTGAGGGC CTTTCCCAAG ACGAAAGCCC AACTCGAGGA 
AGCTCAGTCC GCTCCCCCAT TCGCTGCATC TCACCAGAGC TGGCCAATAC AATTGCTCTG 
AACCCTGGGG GGCGGCCCAA GGAGAGGCAC ATGCACAGCT ACAGAGAAGC GTTTGAGGAA 
ATGGATGGGG GTCCCGTTAG CCCCCCTCCA ATTGTTGGTG GTGAGGTCCT ACCCCAAACC 
CCTGCCTTCC CTGTCTCGCC ACAAACCCCT TACTTCAACA TGTGTCGATC TCCTCCAGGA 
TTGGCTAAAA CACCACTGTC CTTTCTGGGC CTGAAGCCCC ACAACCCTGC TGAGATCCTG 
CTCAACCAGA CAGGCTCAGA GAGTGAGGAT GAGGAAGAGC CTCGTAGTTA TGTGGAGTCT 
GTGGCGAAGG CAGCAGCAGT ACCTGCATCT CCAACATATG ACAGCGACAG CTTAGCTCGT 
CCTGCCGGAC CGTCCCATGC TTACAATGCT CCACTTTCCT CCAGCAGCCC CATTCAGAAC 
CCAGATCCAG TTGAAAGCAC ATTGGCTGAC AGCGGGGTCG GGATGAATTC TTTATCCCAG 
GCTGCTGTCG ACATTTTCCA TTTCCAGCCC TCCGAAATCC CATACCAACC ACCACCACCC 
CCCTACCCAA CCTCCATGTT GGGTAGCTAC ATCAGCCCTT CTCCAGCAGA GGCCATGCAA 
AATAATACCA CCACCATCCA GCCACTTTCT GGAAGTCCTG GGACAAGACA CCACACCATC 
CTTTCTGACA TGGCGTCCGT TCCCAACTTT CAACACAGAT TAGCAAATCA GGATGGTCCC 
ATACTTGGGC GACAGCAGGC TCCAGCTAAT GGTCATGTGC CTGGAATTGC TGGTAGTCCA 
GTGCAAGGAA GACACACTAT GGTGTCCCAG GGTACACAAA GCAGTCCCAT ACTGAGCAGA 
CAGTATCCTG TCACCCAGGG GATTCAGAGT AGTCCAATTC TCAGCAGGCA GCCAATGGGA 
CAAGCTGTCC AGAGCAGTCC AGTCCTCAAC CGACAGGCAT CCCTGAATCA AGCAAACCAA 
AGCAGTCCAG TGTTGAGTAG ACAGCAGTCC ATTACCCTAC CCAACCAAGG CAGCCCGGTC 
CTAAGCAGAC AGTCCTCGCT TAGTCATCCC AGTCAGGGTA GTCCTGTACT AGGACGCCAC 
CCTTCCCTAA CACAGGGTAG CCCCAGCCTG GATAGGCATC CGATGTACAG CGGTTACACC 
ACTCCAGAAG AGCGACACGG AGCTCTGTCA CGCCAAAGCA GCTCTTCAGG ATACCAGCCG 
CCATCTACAC CGTCTTTCCC AGTTTCTCCA GCAGGATACA TCGAGGGAGC GGGATTCAGA 
CAGGGGAGTC CTGCTTTCCA ACCTCAGTTG CCCGAAAAGA GACGCATGTC CAGCAGTGAC 
CGCACCAATG GTGGCCTGTC CTATGGGACG CTCAATGGGA AGATGTCCTC ACCGGTGTCC 
AGTGGCGGCA GCACTCCGAG TGTTCATTTC TTCCACACAC TCCCCGATTT CTCCAAACTC 
AACATGTGTG ATGGAAGTCC AGAAACCAGA ATGAACGTGA AGTTTGTCCA GGACACGTCC 
AAATTCTGGT ATAAACCAGA TATCTCGAGA GACCAAGCGA TTAACATGCT GAAGGATCAA 
GATCCTGGGG CCTTTGTGAT TCGGGACAGT CATTCATTCA GAGGAGCCTA CGGCTTGGCT 
ATGAAAGTGG CTTCACCACC TCCAACCACT CAACCTACAA AGAAAGCTGG AGATGTCACT 
AATGAGTTGG TGAGGCATTT TCTAATTGAG ACGAGTCCGA AGGGTGTGAG ACTGAAGGGC 
TGCCCCAACG AGCCGTACTT TGGCTGCCTG TCTGCGCTGG TCTACCAGCA CTCCATCACT 
CCACTGGCAC TGCCCTGCAA ACTAGTCATA CCAACCAGAG ATCCTCTGGA AGAGTCACCT 
GAGATCGCAA CACCAACCAA CCCGGCGGCA GATATGCTCA AGCAGGGCGC AGGGCAGAAG 
AGTTCAGCTG ATGGCCATGC ATGCAATGTG CTGTACATCA ACTCTGTGGA TATGGAGTCT 
TTGACTGGAC CTCAAGCCAT CGCCAAAGCC ATTTCAGAAA CCATGAACGC CAGTCCGGCA 
CCCTCAGCCA CCATTGTTCA CTTCAAGGTG TCGGCACAAG GCATCACACT CACAGACAAC 
CAGAGAAAGC TTTTCTTCAG ACGTCATTAT CCCATCGGCA CAGTCACGTT CTGTGACATT 
GATCCACAGG AGAGGAAGTG GAACAAACCA GAGGGTGGCA CTGCCAAATT CTTTGGCTTT 
GTGGCTAGAA AGCAAGGCAG CACGACAGAC AATGTCAGTC ACCTGTTTGC TGAGATGGAC 
CCAGATCAGC CGGCCACCGC CATCGTCAAC TTTGTCTCTA AAGTGATGAT CAGCTCTCAG 
AAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1b ( XM_009304966.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303241.1 Danio rerio tensin 1b (tns1b), transcript variant X8, mRNA.

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CloneID	ODa42135
Clone ID Related Accession (Same CDS sequence)	XM_009304966.2
Accession Version	XM_009304966.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tensin-1 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCTC GACGCAGACC GTCAAGGAGT TTGAGTTTGA TCCAGGCCAT GGAGGAGAAC 
TATGAGGTGG ATCTGGTTTA CATCACCGAG CGCATCATTT CTGTCACCTT TCCCAGTAAT 
GCAGAGGAGC CCAGTTACAG CGCCAACATT AAAGAAGTGG CAAACATGCT GCGCTCCAAA 
CATGGAGACA ACTACCTGCT CTTCAACCTC AGTGAAAAGC GCTTGGATAT CTCCAAGCTG 
AATCCCAAAG TTCTGGATTT TGGCTGGCCG GATCATCACG CTCCTGCTTT GGATAAGATC 
TGCAGCATCT GCAAAGCCAT GGACACTTGG ATGAACGCTG GCAGTCACAA CGTGGTGGTC 
ATACACAACA AGGGAAACCG AGGTCGAACT GGGGTGGTTG TGGCTGCGTA CATGCATTAC 
AGCAACATAT CAGCGAGTGC CGACCAGGCT CTGGACAGGT TTGCCATGAA GAGGTTTTAT 
GAAGATAAAG TGCTTCCTGT GGGTCAGCCT TCCCAGAGAA GATATGTGCA GTACTTCAGC 
GGTCTGCTCT CCGGCCACAT TAAGATCAAC AACAAACCCC TGTTTCTCCA TCACGTCATC 
ATGCACGGCA TCCCAAACTT CGAGTCTAAA GGAGGTTGCC GCCCTTTCCT GAAGATCTAC 
CAGGCCATGC AGCCCGTCTA CACATCTGGC ATCTATAATG TCCAAGGAGA CAGTCAGACT 
AGTATCTGCA TCACCATTGA ACCGGGCCTG CTTTTGAAAG GAGACATACT GCTAAAGTGT 
TATCACAAGC GTTTCCGCAG CCCATGCCGT GATGTCATAT TCAGAGTTCA GTTTCACACC 
TGTGCTGTTC ATGATCTGGG TATCGTCTTC GGCAAAGATG AGCTTGATGA AACCTTTAAA 
GACGACAGAT TTCCAGAATA TGGAAAGGTG GAATTCGTCT TTTCTTTTGG CCCAGAAAAA 
ATCCAAGGAA TGGACCACCT TGAGAACGGG CCCAGTGTTT CTGTGGACTA CAATACCCAA 
GATGCACTAA TCCGCTGGGA TTCCTACGAG AACTTCAACC AGCGCTGTGA AGACTCCATG 
GAAGACGTTA TTCACACCGA AGGTCCATTG GATGGTAGTC TCTACGCTAA AGTTCGTAAG 
AAAGAGTCTC TGGAAGGAGG GGTTACAGCC AATGGTCTCC CAGCTGCTGC TGATCACACT 
TTACCTGCTG TAGACCACAC TCTACCACCA GTTGACCACG CCTTGTCTGT AAGCAGTGAT 
TCAGGCAACT CCACAGCGTC CATCAAGACT GACCGCACTG ATGAAGCCAG CCATCAGCCA 
GCATTGGTGA GTCAGCCATC TTCCCAGCCC CTCAGCCCTG AGGAGAAACA GGAGCTGGAC 
CAGTTGCTCA GTGGTTTGGA AGCACCAATG CATCGCACAG GTTACCAAAG CAGCCCTGGT 
GGGGGTGGTG GTGGAGGTGG TGGAGTCCTA CATCTCGTCC CTGCCCAGGT ACACGTCAAT 
GGACACAGCA ACATAGACAG AGAGACAGAT ATCCTTGATG ATGACTTGCC CATCAGCCAG 
GAAGGGAACA GTGTGGACAG TCTTGGAACT CTCTCATCTT TTGAGGGCAG AGCCACTCCT 
GCGGATCTAT ACTACCAGTC AGAGACAGTC ATTAATGGAC AAGATATGCC CTACTTAGAG 
AAGAGTGTCC ATGAGAAAAT GCTAGAGCCC AGAGTTCACA GCTCATCATC TGTCCAAGAG 
CACTTGGGAG CCCCTAGTGG GTATGCTAAC CAGAATGGCA ACATCTATCG ATCTCAGTCA 
TTAGGAGCAA CCCCGAACAC AGACCAGAAG TACATGCCCA AAGCTCCAGC ACGCACAACC 
AGCAGTAGGG ATGCCGTGCA GAGACTGAAT GTTTGGCAAC AATTTGGTGT ACCAGAAGTC 
CCAGTCACAG ATGGGGTCAC CTTCAACCCT GTACTACAAT CCCACAGCCT TCCAGAGTTC 
CCTCAGATGG CATCTCAACA AGAGATTGAG CAGTCCATTG AAGCCCTCAA CATGCTGATG 
CTGGACCTTG ATCCAGCACT TACTCAAGTC CCCAAATCCC ACAGCGCTCC ATCTGGAGAA 
AGTGGTGTGG TCACCACCCA ACCATCTTTC TCTCAAACCC AAGCACGCCC ATCCTACCAG 
GCTGACACTG CTATTCATGA CCGCGGTGCG GCCTCTTCAA TGGGATTTCC TTTGACCTTT 
CAACAAGTAA CTGGAGAGCC TTCACAACCC ACCATGAAAC AGCAGAAACC CACAACTACA 
TCCCAGCATG TCGAATTCGC TTCTGAAGCA GGAGGTCAGG CCCCCATGTC CCAGTCCACA 
GTGCCTTCCT CCACCTTTGG AGTTCTCCAG TTGAAACCCC TCAACATTTA TCCCTCAAGT 
ACCACATCCC AGTCCCCCGA GCCTCAGGAG ACTCAGCGTA GCTACTCTTC TTCCTCATCC 
CCTCTTCCCA AAGAGTCTGA ACCAGAAGAT AGCAGCTACA AATTGGAGGG TCTTGTAGCT 
CACCGTGTAG CTGGACTTCC ATCTGGTGAA ATGTCGCCAG ATGAGGCTGC AGTTCCTGGA 
CGCTGTCGGA CCCTTAGTGA GGGCCTTTCC CAAGACGAAA GCCCAACTCG AGGAAGCTCA 
GTCCGCTCCC CCATTCGCTG CATCTCACCA GAGCTGGCCA ATACAATTGC TCTGAACCCT 
GGGGGGCGGC CCAAGGAGAG GCACATGCAC AGCTACAGAG AAGCGTTTGA GGAAATGGAT 
GGGGGTCCCG TTAGCCCCCC TCCAATTGTT GGTGGTGAGG TCCTACCCCA AACCCCTGCC 
TTCCCTGTCT CGCCACAAAC CCCTTACTTC AACATGTGTC GATCTCCTCC AGGATTGGCT 
AAAACACCAC TGTCCTTTCT GGGCCTGAAG CCCCACAACC CTGCTGAGAT CCTGCTCAAC 
CAGACAGGCT CAGAGAGTGA GGATGAGGAA GAGCCTCGTA GTTATGTGGA GTCTGTGGCG 
AAGGCAGCAG CAGTACCTGC ATCTCCAACA TATGACAGCG ACAGCTTAGC TCGTCCTGCC 
GGACCGTCCC ATGCTTACAA TGCTCCACTT TCCTCCAGCA GCCCCATTCA GAACCCAGAT 
CCAGTTGAAA GCACATTGGC TGACAGCGGG GTCGGGATGA ATTCTTTATC CCAGGCTGCT 
GTCGACATTT TCCATTTCCA GCCCTCCGAA ATCCCATACC AACCACCACC ACCCCCCTAC 
CCAACCTCCA TGTTGGGTAG CTACATCAGC CCTTCTCCAG CAGAGGCCAT GCAAAATAAT 
ACCACCACCA TCCAGCCACT TTCTGGAAGT CCTGGGACAA GACACCACAC CATCCTTTCT 
GACATGGCGT CCGTTCCCAA CTTTCAACAC AGATTAGCAA ATCAGGATGG TCCCATACTT 
GGGCGACAGC AGGCTCCAGC TAATGGTCAT GTGCCTGGAA TTGCTGGTAG TCCAGTGCAA 
GGAAGACACA CTATGGTGTC CCAGGGTACA CAAAGCAGTC CCATACTGAG CAGACAGTAT 
CCTGTCACCC AGGGGATTCA GAGTAGTCCA ATTCTCAGCA GGCAGCCAAT GGGACAAGCT 
GTCCAGAGCA GTCCAGTCCT CAACCGACAG GCATCCCTGA ATCAAGCAAA CCAAAGCAGT 
CCAGTGTTGA GTAGACAGCA GTCCATTACC CTACCCAACC AAGGCAGCCC GGTCCTAAGC 
AGACAGTCCT CGCTTAGTCA TCCCAGTCAG GGTAGTCCTG TACTAGGACG CCACCCTTCC 
CTAACACAGG GTAGCCCCAG CCTGGATAGG CATCCGATGT ACAGCGGTTA CACCACTCCA 
GAAGAGCGAC ACGGAGCTCT GTCACGCCAA AGCAGCTCTT CAGGATACCA GCCGCCATCT 
ACACCGTCTT TCCCAGTTTC TCCAGCAGGA TACATCGAGG GAGCGGGATT CAGACAGGGG 
AGTCCTGCTT TCCAACCTCA GTTGCCCGAA AAGAGACGCA TGTCCAGCAG TGACCGCACC 
AATGGTGGCC TGTCCTATGG GACGCTCAAT GGGAAGATGT CCTCACCGGT GTCCAGTGGC 
GGCAGCACTC CGAGTGTTCA TTTCTTCCAC ACACTCCCCG ATTTCTCCAA ACTCAACATG 
TGTGATGGAA GTCCAGAAAC CAGAATGAAC GTGAAGTTTG TCCAGGACAC GTCCAAATTC 
TGGTATAAAC CAGATATCTC GAGAGACCAA GCGATTAACA TGCTGAAGGA TCAAGATCCT 
GGGGCCTTTG TGATTCGGGA CAGTCATTCA TTCAGAGGAG CCTACGGCTT GGCTATGAAA 
GTGGCTTCAC CACCTCCAAC CACTCAACCT ACAAAGAAAG CTGGAGATGT CACTAATGAG 
TTGGTGAGGC ATTTTCTAAT TGAGACGAGT CCGAAGGGTG TGAGACTGAA GGGCTGCCCC 
AACGAGCCGT ACTTTGGCTG CCTGTCTGCG CTGGTCTACC AGCACTCCAT CACTCCACTG 
GCACTGCCCT GCAAACTAGT CATACCAACC AGAGATCCTC TGGAAGAGTC ACCTGAGATC 
GCAACACCAA CCAACCCGGC GGCAGATATG CTCAAGCAGG GCGCAGGGCA GAAGAGTTCA 
GCTGATGGCC ATGCATGCAA TGTGCTGTAC ATCAACTCTG TGGATATGGA GTCTTTGACT 
GGACCTCAAG CCATCGCCAA AGCCATTTCA GAAACCATGA ACGCCAGTCC GGCACCCTCA 
GCCACCATTG TTCACTTCAA GGTGTCGGCA CAAGGCATCA CACTCACAGA CAACCAGAGA 
AAGCTTTTCT TCAGACGTCA TTATCCCATC GGCACAGTCA CGTTCTGTGA CATTGATCCA 
CAGGAGAGGA AGTGGAACAA ACCAGAGGGT GGCACTGCCA AATTCTTTGG CTTTGTGGCT 
AGAAAGCAAG GCAGCACGAC AGACAATGTC AGTCACCTGT TTGCTGAGAT GGACCCAGAT 
CAGCCGGCCA CCGCCATCGT CAACTTTGTC TCTAAAGTGA TGATCAGCTC TCAGAAGCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303241.1
CDS	1839..6881
Translation	MESRRRPSRSLSLIQAMEENYEVDLVYITERIISVTFPSNAEEPSYSANIKEVANMLRSKHGDNYLLFNLSEKRLDISKLNPKVLDFGWPDHHAPALDKICSICKAMDTWMNAGSHNVVVIHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMKRFYEDKVLPVGQPSQRRYVQYFSGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKRFRSPCRDVIFRVQFHTCAVHDLGIVFGKDELDETFKDDRFPEYGKVEFVFSFGPEKIQGMDHLENGPSVSVDYNTQDALIRWDSYENFNQRCEDSMEDVIHTEGPLDGSLYAKVRKKESLEGGVTANGLPAAADHTLPAVDHTLPPVDHALSVSSDSGNSTASIKTDRTDEASHQPALVSQPSSQPLSPEEKQELDQLLSGLEAPMHRTGYQSSPGGGGGGGGGVLHLVPAQVHVNGHSNIDRETDILDDDLPISQEGNSVDSLGTLSSFEGRATPADLYYQSETVINGQDMPYLEKSVHEKMLEPRVHSSSSVQEHLGAPSGYANQNGNIYRSQSLGATPNTDQKYMPKAPARTTSSRDAVQRLNVWQQFGVPEVPVTDGVTFNPVLQSHSLPEFPQMASQQEIEQSIEALNMLMLDLDPALTQVPKSHSAPSGESGVVTTQPSFSQTQARPSYQADTAIHDRGAASSMGFPLTFQQVTGEPSQPTMKQQKPTTTSQHVEFASEAGGQAPMSQSTVPSSTFGVLQLKPLNIYPSSTTSQSPEPQETQRSYSSSSSPLPKESEPEDSSYKLEGLVAHRVAGLPSGEMSPDEAAVPGRCRTLSEGLSQDESPTRGSSVRSPIRCISPELANTIALNPGGRPKERHMHSYREAFEEMDGGPVSPPPIVGGEVLPQTPAFPVSPQTPYFNMCRSPPGLAKTPLSFLGLKPHNPAEILLNQTGSESEDEEEPRSYVESVAKAAAVPASPTYDSDSLARPAGPSHAYNAPLSSSSPIQNPDPVESTLADSGVGMNSLSQAAVDIFHFQPSEIPYQPPPPPYPTSMLGSYISPSPAEAMQNNTTTIQPLSGSPGTRHHTILSDMASVPNFQHRLANQDGPILGRQQAPANGHVPGIAGSPVQGRHTMVSQGTQSSPILSRQYPVTQGIQSSPILSRQPMGQAVQSSPVLNRQASLNQANQSSPVLSRQQSITLPNQGSPVLSRQSSLSHPSQGSPVLGRHPSLTQGSPSLDRHPMYSGYTTPEERHGALSRQSSSSGYQPPSTPSFPVSPAGYIEGAGFRQGSPAFQPQLPEKRRMSSSDRTNGGLSYGTLNGKMSSPVSSGGSTPSVHFFHTLPDFSKLNMCDGSPETRMNVKFVQDTSKFWYKPDISRDQAINMLKDQDPGAFVIRDSHSFRGAYGLAMKVASPPPTTQPTKKAGDVTNELVRHFLIETSPKGVRLKGCPNEPYFGCLSALVYQHSITPLALPCKLVIPTRDPLEESPEIATPTNPAADMLKQGAGQKSSADGHACNVLYINSVDMESLTGPQAIAKAISETMNASPAPSATIVHFKVSAQGITLTDNQRKLFFRRHYPIGTVTFCDIDPQERKWNKPEGGTAKFFGFVARKQGSTTDNVSHLFAEMDPDQPATAIVNFVSKVMISSQKR

Target ORF information:

RefSeq Version	XM_009304966.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tensin 1b (tns1b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304966.2

ORF Insert Sequence:

ATGGAGTCTC GACGCAGACC GTCAAGGAGT TTGAGTTTGA TCCAGGCCAT GGAGGAGAAC 
TATGAGGTGG ATCTGGTTTA CATCACCGAG CGCATCATTT CTGTCACCTT TCCCAGTAAT 
GCAGAGGAGC CCAGTTACAG CGCCAACATT AAAGAAGTGG CAAACATGCT GCGCTCCAAA 
CATGGAGACA ACTACCTGCT CTTCAACCTC AGTGAAAAGC GCTTGGATAT CTCCAAGCTG 
AATCCCAAAG TTCTGGATTT TGGCTGGCCG GATCATCACG CTCCTGCTTT GGATAAGATC 
TGCAGCATCT GCAAAGCCAT GGACACTTGG ATGAACGCTG GCAGTCACAA CGTGGTGGTC 
ATACACAACA AGGGAAACCG AGGTCGAACT GGGGTGGTTG TGGCTGCGTA CATGCATTAC 
AGCAACATAT CAGCGAGTGC CGACCAGGCT CTGGACAGGT TTGCCATGAA GAGGTTTTAT 
GAAGATAAAG TGCTTCCTGT GGGTCAGCCT TCCCAGAGAA GATATGTGCA GTACTTCAGC 
GGTCTGCTCT CCGGCCACAT TAAGATCAAC AACAAACCCC TGTTTCTCCA TCACGTCATC 
ATGCACGGCA TCCCAAACTT CGAGTCTAAA GGAGGTTGCC GCCCTTTCCT GAAGATCTAC 
CAGGCCATGC AGCCCGTCTA CACATCTGGC ATCTATAATG TCCAAGGAGA CAGTCAGACT 
AGTATCTGCA TCACCATTGA ACCGGGCCTG CTTTTGAAAG GAGACATACT GCTAAAGTGT 
TATCACAAGC GTTTCCGCAG CCCATGCCGT GATGTCATAT TCAGAGTTCA GTTTCACACC 
TGTGCTGTTC ATGATCTGGG TATCGTCTTC GGCAAAGATG AGCTTGATGA AACCTTTAAA 
GACGACAGAT TTCCAGAATA TGGAAAGGTG GAATTCGTCT TTTCTTTTGG CCCAGAAAAA 
ATCCAAGGAA TGGACCACCT TGAGAACGGG CCCAGTGTTT CTGTGGACTA CAATACCCAA 
GATGCACTAA TCCGCTGGGA TTCCTACGAG AACTTCAACC AGCGCTGTGA AGACTCCATG 
GAAGACGTTA TTCACACCGA AGGTCCATTG GATGGTAGTC TCTACGCTAA AGTTCGTAAG 
AAAGAGTCTC TGGAAGGAGG GGTTACAGCC AATGGTCTCC CAGCTGCTGC TGATCACACT 
TTACCTGCTG TAGACCACAC TCTACCACCA GTTGACCACG CCTTGTCTGT AAGCAGTGAT 
TCAGGCAACT CCACAGCGTC CATCAAGACT GACCGCACTG ATGAAGCCAG CCATCAGCCA 
GCATTGGTGA GTCAGCCATC TTCCCAGCCC CTCAGCCCTG AGGAGAAACA GGAGCTGGAC 
CAGTTGCTCA GTGGTTTGGA AGCACCAATG CATCGCACAG GTTACCAAAG CAGCCCTGGT 
GGGGGTGGTG GTGGAGGTGG TGGAGTCCTA CATCTCGTCC CTGCCCAGGT ACACGTCAAT 
GGACACAGCA ACATAGACAG AGAGACAGAT ATCCTTGATG ATGACTTGCC CATCAGCCAG 
GAAGGGAACA GTGTGGACAG TCTTGGAACT CTCTCATCTT TTGAGGGCAG AGCCACTCCT 
GCGGATCTAT ACTACCAGTC AGAGACAGTC ATTAATGGAC AAGATATGCC CTACTTAGAG 
AAGAGTGTCC ATGAGAAAAT GCTAGAGCCC AGAGTTCACA GCTCATCATC TGTCCAAGAG 
CACTTGGGAG CCCCTAGTGG GTATGCTAAC CAGAATGGCA ACATCTATCG ATCTCAGTCA 
TTAGGAGCAA CCCCGAACAC AGACCAGAAG TACATGCCCA AAGCTCCAGC ACGCACAACC 
AGCAGTAGGG ATGCCGTGCA GAGACTGAAT GTTTGGCAAC AATTTGGTGT ACCAGAAGTC 
CCAGTCACAG ATGGGGTCAC CTTCAACCCT GTACTACAAT CCCACAGCCT TCCAGAGTTC 
CCTCAGATGG CATCTCAACA AGAGATTGAG CAGTCCATTG AAGCCCTCAA CATGCTGATG 
CTGGACCTTG ATCCAGCACT TACTCAAGTC CCCAAATCCC ACAGCGCTCC ATCTGGAGAA 
AGTGGTGTGG TCACCACCCA ACCATCTTTC TCTCAAACCC AAGCACGCCC ATCCTACCAG 
GCTGACACTG CTATTCATGA CCGCGGTGCG GCCTCTTCAA TGGGATTTCC TTTGACCTTT 
CAACAAGTAA CTGGAGAGCC TTCACAACCC ACCATGAAAC AGCAGAAACC CACAACTACA 
TCCCAGCATG TCGAATTCGC TTCTGAAGCA GGAGGTCAGG CCCCCATGTC CCAGTCCACA 
GTGCCTTCCT CCACCTTTGG AGTTCTCCAG TTGAAACCCC TCAACATTTA TCCCTCAAGT 
ACCACATCCC AGTCCCCCGA GCCTCAGGAG ACTCAGCGTA GCTACTCTTC TTCCTCATCC 
CCTCTTCCCA AAGAGTCTGA ACCAGAAGAT AGCAGCTACA AATTGGAGGG TCTTGTAGCT 
CACCGTGTAG CTGGACTTCC ATCTGGTGAA ATGTCGCCAG ATGAGGCTGC AGTTCCTGGA 
CGCTGTCGGA CCCTTAGTGA GGGCCTTTCC CAAGACGAAA GCCCAACTCG AGGAAGCTCA 
GTCCGCTCCC CCATTCGCTG CATCTCACCA GAGCTGGCCA ATACAATTGC TCTGAACCCT 
GGGGGGCGGC CCAAGGAGAG GCACATGCAC AGCTACAGAG AAGCGTTTGA GGAAATGGAT 
GGGGGTCCCG TTAGCCCCCC TCCAATTGTT GGTGGTGAGG TCCTACCCCA AACCCCTGCC 
TTCCCTGTCT CGCCACAAAC CCCTTACTTC AACATGTGTC GATCTCCTCC AGGATTGGCT 
AAAACACCAC TGTCCTTTCT GGGCCTGAAG CCCCACAACC CTGCTGAGAT CCTGCTCAAC 
CAGACAGGCT CAGAGAGTGA GGATGAGGAA GAGCCTCGTA GTTATGTGGA GTCTGTGGCG 
AAGGCAGCAG CAGTACCTGC ATCTCCAACA TATGACAGCG ACAGCTTAGC TCGTCCTGCC 
GGACCGTCCC ATGCTTACAA TGCTCCACTT TCCTCCAGCA GCCCCATTCA GAACCCAGAT 
CCAGTTGAAA GCACATTGGC TGACAGCGGG GTCGGGATGA ATTCTTTATC CCAGGCTGCT 
GTCGACATTT TCCATTTCCA GCCCTCCGAA ATCCCATACC AACCACCACC ACCCCCCTAC 
CCAACCTCCA TGTTGGGTAG CTACATCAGC CCTTCTCCAG CAGAGGCCAT GCAAAATAAT 
ACCACCACCA TCCAGCCACT TTCTGGAAGT CCTGGGACAA GACACCACAC CATCCTTTCT 
GACATGGCGT CCGTTCCCAA CTTTCAACAC AGATTAGCAA ATCAGGATGG TCCCATACTT 
GGGCGACAGC AGGCTCCAGC TAATGGTCAT GTGCCTGGAA TTGCTGGTAG TCCAGTGCAA 
GGAAGACACA CTATGGTGTC CCAGGGTACA CAAAGCAGTC CCATACTGAG CAGACAGTAT 
CCTGTCACCC AGGGGATTCA GAGTAGTCCA ATTCTCAGCA GGCAGCCAAT GGGACAAGCT 
GTCCAGAGCA GTCCAGTCCT CAACCGACAG GCATCCCTGA ATCAAGCAAA CCAAAGCAGT 
CCAGTGTTGA GTAGACAGCA GTCCATTACC CTACCCAACC AAGGCAGCCC GGTCCTAAGC 
AGACAGTCCT CGCTTAGTCA TCCCAGTCAG GGTAGTCCTG TACTAGGACG CCACCCTTCC 
CTAACACAGG GTAGCCCCAG CCTGGATAGG CATCCGATGT ACAGCGGTTA CACCACTCCA 
GAAGAGCGAC ACGGAGCTCT GTCACGCCAA AGCAGCTCTT CAGGATACCA GCCGCCATCT 
ACACCGTCTT TCCCAGTTTC TCCAGCAGGA TACATCGAGG GAGCGGGATT CAGACAGGGG 
AGTCCTGCTT TCCAACCTCA GTTGCCCGAA AAGAGACGCA TGTCCAGCAG TGACCGCACC 
AATGGTGGCC TGTCCTATGG GACGCTCAAT GGGAAGATGT CCTCACCGGT GTCCAGTGGC 
GGCAGCACTC CGAGTGTTCA TTTCTTCCAC ACACTCCCCG ATTTCTCCAA ACTCAACATG 
TGTGATGGAA GTCCAGAAAC CAGAATGAAC GTGAAGTTTG TCCAGGACAC GTCCAAATTC 
TGGTATAAAC CAGATATCTC GAGAGACCAA GCGATTAACA TGCTGAAGGA TCAAGATCCT 
GGGGCCTTTG TGATTCGGGA CAGTCATTCA TTCAGAGGAG CCTACGGCTT GGCTATGAAA 
GTGGCTTCAC CACCTCCAAC CACTCAACCT ACAAAGAAAG CTGGAGATGT CACTAATGAG 
TTGGTGAGGC ATTTTCTAAT TGAGACGAGT CCGAAGGGTG TGAGACTGAA GGGCTGCCCC 
AACGAGCCGT ACTTTGGCTG CCTGTCTGCG CTGGTCTACC AGCACTCCAT CACTCCACTG 
GCACTGCCCT GCAAACTAGT CATACCAACC AGAGATCCTC TGGAAGAGTC ACCTGAGATC 
GCAACACCAA CCAACCCGGC GGCAGATATG CTCAAGCAGG GCGCAGGGCA GAAGAGTTCA 
GCTGATGGCC ATGCATGCAA TGTGCTGTAC ATCAACTCTG TGGATATGGA GTCTTTGACT 
GGACCTCAAG CCATCGCCAA AGCCATTTCA GAAACCATGA ACGCCAGTCC GGCACCCTCA 
GCCACCATTG TTCACTTCAA GGTGTCGGCA CAAGGCATCA CACTCACAGA CAACCAGAGA 
AAGCTTTTCT TCAGACGTCA TTATCCCATC GGCACAGTCA CGTTCTGTGA CATTGATCCA 
CAGGAGAGGA AGTGGAACAA ACCAGAGGGT GGCACTGCCA AATTCTTTGG CTTTGTGGCT 
AGAAAGCAAG GCAGCACGAC AGACAATGTC AGTCACCTGT TTGCTGAGAT GGACCCAGAT 
CAGCCGGCCA CCGCCATCGT CAACTTTGTC TCTAAAGTGA TGATCAGCTC TCAGAAGCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	tns1b
Entrez Gene ID	555362
Full Name	tensin 1b
Synonyms	fa11e05,si:ch211-148j1.1,tns1,wu:fa11e05
General protein information	Preferred Names tensin 1b Names tensin 1b tensin-1 tensin 1 tensin-like C1 domain-containing phosphatase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

	LOC696960	XP_002808070.1
Bos taurus (cattle)	TNS1	NP_777191.1
Danio rerio (zebrafish)	tns1	XP_003199292.2
Xenopus tropicalis (tropical clawed frog)	tns1	XP_004918285.1
Homo sapiens (human)	TNS1	NP_072174.3
Pan troglodytes (chimpanzee)	TNS1	XP_516080.3
Mus musculus (house mouse)	Tns1	NP_082160.3
Canis lupus familiaris (dog)	TNS1	XP_005640681.1
Gallus gallus (chicken)	TNS1	NP_990786.1
Rattus norvegicus (Norway rat)	Tns1	NP_001178739.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

tns1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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