Tns1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tns1
Entrez Gene ID	21961
Full Name	tensin 1
Synonyms	1110018I21Rik,1200014E20Rik,AI648117,E030018G17Rik,E030037J05Rik,Tns
General protein information	Preferred Names tensin 1 Names tensin-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 C3\|1 38.17 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006495880.2	XP_006495943.1	tensin-1 isoform X19
NM_001289895.1	NP_001276824.1	tensin-1 isoform b
XM_006495867.2	XP_006495930.2	tensin-1 isoform X1
XM_017319856.1	XP_017175345.1	tensin-1 isoform X5
XM_017319862.2	XP_017175351.1	tensin-1 isoform X11
XM_006495873.2	XP_006495936.2	tensin-1 isoform X16
XM_030252984.1	XP_030108844.1	tensin-1 isoform X20
XM_011238476.2	XP_011236778.1	tensin-1 isoform X22
XM_006495880.3	XP_006495943.1	tensin-1 isoform X22
XM_011238477.1	XP_011236779.1	tensin-1 isoform X22
XM_006495881.3	XP_006495944.1	tensin-1 isoform X22
XM_030252987.1	XP_030108847.1	tensin-1 isoform X26
NM_027884.3	NP_082160.3	tensin-1 isoform a
XM_006495877.3	XP_006495940.1	tensin-1 isoform X19
XM_006495881.2	XP_006495944.1	tensin-1 isoform X19
XM_006495883.1	XP_006495946.1	tensin-1 isoform X20
XM_017319847.1	XP_017175336.1	tensin-1 isoform X2
XM_017319853.1	XP_017175342.1	tensin-1 isoform X4
XM_030252982.1	XP_030108842.1	tensin-1 isoform X7
XM_017319859.1	XP_017175348.1	tensin-1 isoform X9
XM_017319864.1	XP_017175353.1	tensin-1 isoform X13
XM_006495872.1	XP_006495935.1	tensin-1 isoform X14
XM_006495874.4	XP_006495937.1	tensin-1 isoform X17
XM_030252986.1	XP_030108846.1	tensin-1 isoform X25
XM_030252988.1	XP_030108848.1	tensin-1 isoform X27
NM_027884.4	NP_082160.3	tensin-1 isoform a
XM_006495874.3	XP_006495937.1	tensin-1 isoform X15
XM_017319860.1	XP_017175349.1	tensin-1 isoform X10
XM_017319863.1	XP_017175352.1	tensin-1 isoform X12
XM_030252983.1	XP_030108843.1	tensin-1 isoform X15
XM_006495875.2	XP_006495938.1	tensin-1 isoform X18
XM_030252985.1	XP_030108845.1	tensin-1 isoform X23
XM_006495883.2	XP_006495946.1	tensin-1 isoform X24
XM_017319862.1	XP_017175351.1	tensin-1 isoform X10
XM_011238476.1	XP_011236778.1	tensin-1 isoform X19
XM_017319848.1	XP_017175337.1	tensin-1 isoform X3
XM_017319865.1	XP_017175354.1	tensin-1 isoform X19
XM_017319866.1	XP_017175355.1	tensin-1 isoform X21
XM_006495877.4	XP_006495940.1	tensin-1 isoform X22
XM_006495879.1	XP_006495942.1	tensin-1 isoform X22
XM_006495871.3	XP_006495934.1	tensin-1 isoform X6
NM_001289895.2	NP_001276824.1	tensin-1 isoform b
XM_006495871.4	XP_006495934.1	tensin-1 isoform X6
XM_017319858.1	XP_017175347.1	tensin-1 isoform X8
XM_006495878.3	XP_006495941.1	tensin-1 isoform X22
XM_006495882.3	XP_006495945.1	tensin-1 isoform X22

Pathways from BioSystems

Homologies

	LOC696960	XP_002808070.1
Bos taurus (cattle)	TNS1	NP_777191.1
Danio rerio (zebrafish)	tns1	XP_003199292.2
Xenopus tropicalis (tropical clawed frog)	tns1	XP_004918285.1
Homo sapiens (human)	TNS1	NP_072174.3
Pan troglodytes (chimpanzee)	TNS1	XP_516080.3
Mus musculus (house mouse)	Tns1	NP_082160.3
Canis lupus familiaris (dog)	TNS1	XP_005640681.1
Gallus gallus (chicken)	TNS1	NP_990786.1
Rattus norvegicus (Norway rat)	Tns1	NP_001178739.1

Gene Ontology
Bibliography

Related articles in PubMed

Tensin1 positively regulates RhoA activity through its interaction with DLC1.
Shih YP, Sun P, Wang A, Lo SH
Biochimica et biophysica acta1853(12)3258-65(2015 Dec)

Genetic association analyses highlight biological pathways underlying mitral valve prolapse.
Dina C, Bouatia-Naji N, Tucker N, Delling FN, Toomer K, Durst R, Perrocheau M, Fernandez-Friera L, Solis J, , Le Tourneau T, Chen MH, Probst V, Bosse Y, Pibarot P, Zelenika D, Lathrop M, Hercberg S, Roussel R, Benjamin EJ, Bonnet F, Lo SH, Dolmatova E, Simonet F, Lecointe S, Kyndt F, Redon R, Le Marec H, Froguel P, Ellinor PT, Vasan RS, Bruneval P, Markwald RR, Norris RA, Milan DJ, Slaugenhaupt SA, Levine RA, Schott JJ, Hagege AA, , Jeunemaitre X,
Nature genetics47(10)1206-11(2015 Oct)

Endothelial cell migration during murine yolk sac vascular remodeling occurs by means of a Rac1 and FAK activation pathway in vivo.
Enciso JM, Konecny CM, Karpen HE, Hirschi KK
Developmental dynamics : an official publication of the American Association of Anatomists239(10)2570-83(2010 Oct)

Rhombomere-specific analysis reveals the repertoire of genetic cues expressed across the developing hindbrain.
Chambers D, Wilson LJ, Alfonsi F, Hunter E, Saxena U, Blanc E, Lumsden A
Neural development46(2009 Feb)

Tagging genes with cassette-exchange sites.
Cobellis G, Nicolaus G, Iovino M, Romito A, Marra E, Barbarisi M, Sardiello M, Di Giorgio FP, Iovino N, Zollo M, Ballabio A, Cortese R
Nucleic acids research33(4)e44(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

studies demonstrate that TNS1 binds to DLC1 and fine-tunes its RhoGAP activity toward RhoA and that the TNS1-DLC1-RhoA signaling axis is critical in regulating cellular functions that lead to angiogenesis
Title: Tensin1 positively regulates RhoA activity through its interaction with DLC1.

The following Tns1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tns1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu27366	XM_006495883.2 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu85108	XM_030252986.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu85109	XM_030252987.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu85110	XM_030252988.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu27359	XM_006495871.4 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu27362	XM_006495874.4 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu27365	XM_011238476.2 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu27365	XM_006495880.3 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu27365	XM_006495877.4 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu27365	XM_006495881.3 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77072	XM_017319847.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77073	XM_017319848.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77074	XM_017319853.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77075	XM_017319856.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77076	XM_017319858.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77077	XM_017319859.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77078	XM_017319860.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77079	XM_017319862.2 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77080	XM_017319863.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77081	XM_017319864.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77082	XM_017319865.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu77083	XM_017319866.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu85104	XM_030252982.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu85105	XM_030252983.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu85106	XM_030252984.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu85107	XM_030252985.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu27360	XM_006495872.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu27363	XM_006495875.2 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu27365	XM_011238477.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu27365	XM_006495879.1 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu27365	XM_006495878.3 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu27365	XM_006495882.3 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu68935	XM_006495867.2 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu68936	XM_006495873.2 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu04724	NM_027884.4 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1385.30 $1979.00
OMu58480	NM_001289895.2 Latest version!	Mus musculus tensin 1 (Tns1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OMu27365	XM_006495880.2	Mus musculus tensin 1 (Tns1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu27365	XM_006495881.2	Mus musculus tensin 1 (Tns1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu04724	NM_027884.3	Mus musculus tensin 1 (Tns1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1385.30 $1979.00
OMu27362	XM_006495874.3	Mus musculus tensin 1 (Tns1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu27366	XM_006495883.1	Mus musculus tensin 1 (Tns1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu77079	XM_017319862.1	Mus musculus tensin 1 (Tns1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu27365	XM_011238476.1	Mus musculus tensin 1 (Tns1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu27365	XM_006495877.3	Mus musculus tensin 1 (Tns1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu58480	NM_001289895.1	Mus musculus tensin 1 (Tns1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu27359	XM_006495871.3	Mus musculus tensin 1 (Tns1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tns1 ( XM_006495883.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495946.1 Mus musculus tensin 1 (Tns1), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OMu27366
Clone ID Related Accession (Same CDS sequence)	XM_006495883.1 , XM_006495883.2
Accession Version	XM_006495883.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495883.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCGGG CTCAGCAACT CAGATCCCAT CCAGGCGGGG GCCCGACTGT GCCCTCCCCT 
GGCCGCCACA TTGTCCCAGC CCAGGTTCAC GTCAATGGTG GGGCCCTGGC ATCTGAGCGG 
GAGACAGACA TTCTAGATGA TGAGCTGCCC ATCCAGGACG GGCAGAGTGG GGGCAGCATG 
GGAACGCTGT CCTCCCTGGA TGGGGTCACC AACACCAGTG AAAGTGGCTA CCCAGAGACC 
CTGTCCCCAC TGACCAATGG CCTGGACAAA CCCTATTCCA CAGAACCTGT GCTCAACGGC 
GGAGGCTACC CCTATGAGGC TGCCAACCGG GTGATTCCTG TCCACAGCAG TCACTCGGCC 
CCCATTCGAC CCTCCTACTC TGCTCAGGAG GGTTTAGCTG GCTACCAGCG TGAGGGACCC 
CACCCAGCCT GGTCACAGCA AGTGACCAGT GCCCACTGTG GCTGTGACCC CAGTGGTCTA 
TTCAGATCCC AGTCCTTTCC AGATGTGGAG CCCCAGCTGC CCCAAGCTCC AACCCGTGGG 
GGTAGTAGCC GAGAGGCTGT GCAGAGGGGT CTGAATTCAT GGCAACAGCA GCAGCCCCAC 
CCACCTCCCC GCCAGCAGGA GAGATCCCCA CTGCAGAGTC TTGCCCGCAG CAAGCCTAGC 
CCCCAGCTGT CAGCAGAGAC CCCCGTAGCT GCTCTCCCAG AGTTCCCAAG GGCAGCGTCC 
CAGCAGGAGA TCGAGCAGTC CATCGAAACC CTCAATATGT TGATGCTGGA CCTGGAGCCA 
GCCTCCGCAG CTGCCCCTCT TCATAAGTCC CAGAGTGTCC CTGGGGCCTG GCCAGGGGCT 
TCTCCACTCT CTTCCCAGCC CCTCTTGGGG TCTTCCCGCC AGTCCCACCC ACTTACCCAG 
TCCCGATCTG GCTATATCCC CAGTGGGCAT TCTCTGGGAA CCCCGGAGCT GGTCTCTTCT 
GGCAGGCCTT ATTCACCTTA CGATTATCAG CTGCATCCAG CTGGATCCAA CCAGAGTTTC 
CATCCAAAAA GTCCAGCCTC TTCCACCTTC CTTCCAAGCC CTCATAGCTC TGCGGGGCCT 
CAGGAGCCCC CAGCCTCTCT CCCTGGTCTC ATCGCTCAGC CCCAGCTCCC ACCAAAGGAA 
ACTACATCAG ATCCCTCCCG AACTCCAGAG GAAGAGCCGC TGAACCTGGA AGGGCTGGTA 
GCCCATCGGG TAGCAGTGTA CAATGCCAGA CTACAGAGCC TGAGGCAGAG CCGCGGCTTC 
CAACCCTCTC CCCACTGGCG CCGATCTGCC TCCCTCTCTG GGGTTCAGGC TCGGGAGAGG 
CAGCCTGCAG AGCCCCCAGG CCCACTCCGG AGGCGGGCAG CCAGTGATGG ACAGTATGAA 
AACCAGTCTC CGGAGGCCAC ATCCCCCCGT AGTCCTGGGG TCCGCTCCCC CGTCCAGTGC 
GTCTCCCCTG AGCTGGCTCT TACCATTGCC CTCAATCCTG GAGGGCGCCC CAAAGAGCCT 
CATTTGCATA GCTACAAGGA GGCCTTTGAG GAGATGGAGG GGACCTCCCC AAGCAGCCCG 
CCACACAGTG TGGCGCGCTC TCCTCCAGGT CTGGCCAAGA CACCCCTGTC TGCTCTGGGC 
CTGAAACCCC ACAACCCAGC AGACATCTTG CTGCACCCCA CAGGTGTTGC CAGAAGACTG 
ATCCAGCCAG AGGAAGACGA GGGGGAGGAG GTGACTAAGC CTCCAGAAGA GCCCCGGAGC 
TATGTGGAGT CCGTGGCGAG GACAGCGGTG GCAGGGCCTC GAGCTCAGGA CGTTGAGCCT 
AAGAGCTTCA GCGCACCAGC TGCTCACGCC TATGGGCACG AGACACCCCT GAGGAACGGG 
ACCCCAGGGG GCTCCTTTGT CTCCCCGAGC CCCCTCTCCA CAAGCAGCCC CATCCTCAGT 
GCTGACAGCA CTTCCGTGGG CAGTTTCCCA TCAGTGGTGA GCAGTGACCA GGGTCCCCGG 
ACACCCTTCC AGCCTATGCT GGACTCCAGC ATCCGCTCAG GCAGCTTGGG GCAGCCGAGC 
CCTGCAGCAC TGAGTTATCA GAGCTCCTCG CCTGTCCCTG TTGGTGGCAG CAGCTACAAC 
AGCCCTGACT ACTCCCTCCA ACCATTCAGC TCTTCTCCAG AAAGCCAAGG CCAGCCACAG 
TACAGTGCGG CCAGTGTCCA CATGGTACCT GGGAGCCCTC AGGCACGACA CAGGACAGTG 
GGTACCAACA CTCCACCTAG CCCTGGTTTT GGCCGGCGGG CCGTCAACCC CACCATGGCT 
GCCCCTGGTA GTCCCAGTTT GAGCCATCGG CAGGTGATGG GTCCATCTGG TCCAGGTTTC 
CATGGTAACG TGGTTTCTGG CCACCCAGCC AGTGCAGCTA CCACTCCTGG AAGCCCCAGC 
CTGGGCCGGC ACCCAGTGGG AAGCCATCAA GTTCCAGGCC TCCACAGCAG TGTGGTCACC 
ACTCCGGGAA GCCCAAGCCT GGGCCGACAC CCTGGTGCCC ACCAAGGGAA TCTGGCCTCC 
AGTCTCCACA GCAATGCAGT CATCAGCCCT GGGAGCCCAA GCTTGGGCCG GCACCTGGGT 
GGGTCTGGAT CCGTGGTTCC TGGAAGCCCC AGCTTGGACC GGCATGCAGC TTATGGTGGC 
TACTCTACCC CTGAGGACCG AAGACCAACA CTGTCCCGGC AAAGCAGTGC CTCTGGCTAC 
CAGGCCCCAT CCACACCGTC ATTCCCTGTC TCTCCTGCCT ACTACCCTGG CCTGAGCAGC 
CCTGCCACCT CACCCTCGCC AGACTCAGCA GCCTTCCGGC AGGGGAGCCC CACACCAGCC 
TTACCAGAGA AGCGAAGAAT GTCAGTGGGA GACCGGGCAG GCAGCCTCCC CAACTATGCC 
ACCATCAATG GGAAAGTGTC TTCCTCGCCT GTCGCCAATG GCATGGCCAG TGGGAGCAGC 
ACCGTCTCCT TCTCTCACAC GCTTCCGGAC TTTTCTAAGT ATTCGATGCC AGACAATAGT 
CCGGAGACTC GGGCTAAAGT GAAGTTTGTC CAGGACACTT CCAAGTATTG GTACAAGCCT 
GAGATCTCCA GAGAACAGGC CATCGCACTC CTAAAGGACC AGGAGCCAGG AGCCTTCATC 
ATCCGGGACA GCCACTCCTT CCGAGGGGCT TATGGGCTGG CCATGAAGGT GTCCTCACCC 
CCTCCAACCA TCACTCAGCA GGGCAAGAAA GGAGACATGA CCCATGAGCT GGTGAGGCAC 
TTTCTGATCG AGACAGGCCC TAGAGGAGTG AAGCTCAAGG GTTGTCCCAA TGAGCCAAAC 
TTTGGTTCCC TTTCTGCCCT GGTCTATCAG CACTCCGTCA TCCCACTGGC CCTGCCCTGT 
AAACTGGTCA TTCCAAGCCG AGACCCCACA GATGAATCAA AAGATAGCTC AGGCCCTGCC 
AACTCAACCA CTGACCTGCT GAAGCAAGGA GCAGCTTGCA ATGTGCTCTT CGTCAACTCT 
GTGGATATGG AGTCACTCAC CGGGCCACAG GCCATTTCCA AAGCCACATC TGAGACTCTG 
GCTGCCGACC CCACACCGGC TGCCACCATT GTTCACTTCA AGGTCTCTGC CCAAGGAATC 
ACACTGACTG ACAATCAAAG AAAGCTCTTC TTTAGAAGAC ACTACCCCCT CAACACTGTC 
ACCTTCTGTG ACCTGGATCC ACAAGAAAGA AAGTGGATGA AGACAGAGGG AGGCGCCCCT 
GCTAAGCTCT TTGGCTTCGT GGCCCGGAAG CAGGGCAGCA CCACAGACAA CGCTTGCCAC 
CTCTTCGCAG AGCTTGACCC CAACCAGCCT GCGTCTGCCA TCGTCAACTT CGTCTCCAAG 
GTCATGCTGA GTGCTGGCCA GAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495946.1
CDS	454..4323
Translation	MYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495883.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495883.2

ORF Insert Sequence:

ATGTACCGGG CTCAGCAACT CAGATCCCAT CCAGGCGGGG GCCCGACTGT GCCCTCCCCT 
GGCCGCCACA TTGTCCCAGC CCAGGTTCAC GTCAATGGTG GGGCCCTGGC ATCTGAGCGG 
GAGACAGACA TTCTAGATGA TGAGCTGCCC ATCCAGGACG GGCAGAGTGG GGGCAGCATG 
GGAACGCTGT CCTCCCTGGA TGGGGTCACC AACACCAGTG AAAGTGGCTA CCCAGAGACC 
CTGTCCCCAC TGACCAATGG CCTGGACAAA CCCTATTCCA CAGAACCTGT GCTCAACGGC 
GGAGGCTACC CCTATGAGGC TGCCAACCGG GTGATTCCTG TCCACAGCAG TCACTCGGCC 
CCCATTCGAC CCTCCTACTC TGCTCAGGAG GGTTTAGCTG GCTACCAGCG TGAGGGACCC 
CACCCAGCCT GGTCACAGCA AGTGACCAGT GCCCACTGTG GCTGTGACCC CAGTGGTCTA 
TTCAGATCCC AGTCCTTTCC AGATGTGGAG CCCCAGCTGC CCCAAGCTCC AACCCGTGGG 
GGTAGTAGCC GAGAGGCTGT GCAGAGGGGT CTGAATTCAT GGCAACAGCA GCAGCCCCAC 
CCACCTCCCC GCCAGCAGGA GAGATCCCCA CTGCAGAGTC TTGCCCGCAG CAAGCCTAGC 
CCCCAGCTGT CAGCAGAGAC CCCCGTAGCT GCTCTCCCAG AGTTCCCAAG GGCAGCGTCC 
CAGCAGGAGA TCGAGCAGTC CATCGAAACC CTCAATATGT TGATGCTGGA CCTGGAGCCA 
GCCTCCGCAG CTGCCCCTCT TCATAAGTCC CAGAGTGTCC CTGGGGCCTG GCCAGGGGCT 
TCTCCACTCT CTTCCCAGCC CCTCTTGGGG TCTTCCCGCC AGTCCCACCC ACTTACCCAG 
TCCCGATCTG GCTATATCCC CAGTGGGCAT TCTCTGGGAA CCCCGGAGCT GGTCTCTTCT 
GGCAGGCCTT ATTCACCTTA CGATTATCAG CTGCATCCAG CTGGATCCAA CCAGAGTTTC 
CATCCAAAAA GTCCAGCCTC TTCCACCTTC CTTCCAAGCC CTCATAGCTC TGCGGGGCCT 
CAGGAGCCCC CAGCCTCTCT CCCTGGTCTC ATCGCTCAGC CCCAGCTCCC ACCAAAGGAA 
ACTACATCAG ATCCCTCCCG AACTCCAGAG GAAGAGCCGC TGAACCTGGA AGGGCTGGTA 
GCCCATCGGG TAGCAGTGTA CAATGCCAGA CTACAGAGCC TGAGGCAGAG CCGCGGCTTC 
CAACCCTCTC CCCACTGGCG CCGATCTGCC TCCCTCTCTG GGGTTCAGGC TCGGGAGAGG 
CAGCCTGCAG AGCCCCCAGG CCCACTCCGG AGGCGGGCAG CCAGTGATGG ACAGTATGAA 
AACCAGTCTC CGGAGGCCAC ATCCCCCCGT AGTCCTGGGG TCCGCTCCCC CGTCCAGTGC 
GTCTCCCCTG AGCTGGCTCT TACCATTGCC CTCAATCCTG GAGGGCGCCC CAAAGAGCCT 
CATTTGCATA GCTACAAGGA GGCCTTTGAG GAGATGGAGG GGACCTCCCC AAGCAGCCCG 
CCACACAGTG TGGCGCGCTC TCCTCCAGGT CTGGCCAAGA CACCCCTGTC TGCTCTGGGC 
CTGAAACCCC ACAACCCAGC AGACATCTTG CTGCACCCCA CAGGTGTTGC CAGAAGACTG 
ATCCAGCCAG AGGAAGACGA GGGGGAGGAG GTGACTAAGC CTCCAGAAGA GCCCCGGAGC 
TATGTGGAGT CCGTGGCGAG GACAGCGGTG GCAGGGCCTC GAGCTCAGGA CGTTGAGCCT 
AAGAGCTTCA GCGCACCAGC TGCTCACGCC TATGGGCACG AGACACCCCT GAGGAACGGG 
ACCCCAGGGG GCTCCTTTGT CTCCCCGAGC CCCCTCTCCA CAAGCAGCCC CATCCTCAGT 
GCTGACAGCA CTTCCGTGGG CAGTTTCCCA TCAGTGGTGA GCAGTGACCA GGGTCCCCGG 
ACACCCTTCC AGCCTATGCT GGACTCCAGC ATCCGCTCAG GCAGCTTGGG GCAGCCGAGC 
CCTGCAGCAC TGAGTTATCA GAGCTCCTCG CCTGTCCCTG TTGGTGGCAG CAGCTACAAC 
AGCCCTGACT ACTCCCTCCA ACCATTCAGC TCTTCTCCAG AAAGCCAAGG CCAGCCACAG 
TACAGTGCGG CCAGTGTCCA CATGGTACCT GGGAGCCCTC AGGCACGACA CAGGACAGTG 
GGTACCAACA CTCCACCTAG CCCTGGTTTT GGCCGGCGGG CCGTCAACCC CACCATGGCT 
GCCCCTGGTA GTCCCAGTTT GAGCCATCGG CAGGTGATGG GTCCATCTGG TCCAGGTTTC 
CATGGTAACG TGGTTTCTGG CCACCCAGCC AGTGCAGCTA CCACTCCTGG AAGCCCCAGC 
CTGGGCCGGC ACCCAGTGGG AAGCCATCAA GTTCCAGGCC TCCACAGCAG TGTGGTCACC 
ACTCCGGGAA GCCCAAGCCT GGGCCGACAC CCTGGTGCCC ACCAAGGGAA TCTGGCCTCC 
AGTCTCCACA GCAATGCAGT CATCAGCCCT GGGAGCCCAA GCTTGGGCCG GCACCTGGGT 
GGGTCTGGAT CCGTGGTTCC TGGAAGCCCC AGCTTGGACC GGCATGCAGC TTATGGTGGC 
TACTCTACCC CTGAGGACCG AAGACCAACA CTGTCCCGGC AAAGCAGTGC CTCTGGCTAC 
CAGGCCCCAT CCACACCGTC ATTCCCTGTC TCTCCTGCCT ACTACCCTGG CCTGAGCAGC 
CCTGCCACCT CACCCTCGCC AGACTCAGCA GCCTTCCGGC AGGGGAGCCC CACACCAGCC 
TTACCAGAGA AGCGAAGAAT GTCAGTGGGA GACCGGGCAG GCAGCCTCCC CAACTATGCC 
ACCATCAATG GGAAAGTGTC TTCCTCGCCT GTCGCCAATG GCATGGCCAG TGGGAGCAGC 
ACCGTCTCCT TCTCTCACAC GCTTCCGGAC TTTTCTAAGT ATTCGATGCC AGACAATAGT 
CCGGAGACTC GGGCTAAAGT GAAGTTTGTC CAGGACACTT CCAAGTATTG GTACAAGCCT 
GAGATCTCCA GAGAACAGGC CATCGCACTC CTAAAGGACC AGGAGCCAGG AGCCTTCATC 
ATCCGGGACA GCCACTCCTT CCGAGGGGCT TATGGGCTGG CCATGAAGGT GTCCTCACCC 
CCTCCAACCA TCACTCAGCA GGGCAAGAAA GGAGACATGA CCCATGAGCT GGTGAGGCAC 
TTTCTGATCG AGACAGGCCC TAGAGGAGTG AAGCTCAAGG GTTGTCCCAA TGAGCCAAAC 
TTTGGTTCCC TTTCTGCCCT GGTCTATCAG CACTCCGTCA TCCCACTGGC CCTGCCCTGT 
AAACTGGTCA TTCCAAGCCG AGACCCCACA GATGAATCAA AAGATAGCTC AGGCCCTGCC 
AACTCAACCA CTGACCTGCT GAAGCAAGGA GCAGCTTGCA ATGTGCTCTT CGTCAACTCT 
GTGGATATGG AGTCACTCAC CGGGCCACAG GCCATTTCCA AAGCCACATC TGAGACTCTG 
GCTGCCGACC CCACACCGGC TGCCACCATT GTTCACTTCA AGGTCTCTGC CCAAGGAATC 
ACACTGACTG ACAATCAAAG AAAGCTCTTC TTTAGAAGAC ACTACCCCCT CAACACTGTC 
ACCTTCTGTG ACCTGGATCC ACAAGAAAGA AAGTGGATGA AGACAGAGGG AGGCGCCCCT 
GCTAAGCTCT TTGGCTTCGT GGCCCGGAAG CAGGGCAGCA CCACAGACAA CGCTTGCCAC 
CTCTTCGCAG AGCTTGACCC CAACCAGCCT GCGTCTGCCA TCGTCAACTT CGTCTCCAAG 
GTCATGCTGA GTGCTGGCCA GAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_030252986.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108846.1 Mus musculus tensin 1 (Tns1), transcript variant X32, mRNA.

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CloneID	OMu85108
Clone ID Related Accession (Same CDS sequence)	XM_030252986.1
Accession Version	XM_030252986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCGGG CTCAGCAACT CAGATCCCAT CCAGGCGGGG GCCCGACTGT GCCCTCCCCT 
GGCCGCCACA TTGTCCCAGC CCAGGTTCAC GTCAATGGTG GGGCCCTGGC ATCTGAGCGG 
GAGACAGACA TTCTAGATGA TGAGCTGCCC ATCCAGGACG GGCAGAGTGG GGGCAGCATG 
GGAACGCTGT CCTCCCTGGA TGGGGTCACC AACACCAGTG AAAGTGGCTA CCCAGAGACC 
CTGTCCCCAC TGACCAATGG CCTGGACAAA CCCTATTCCA CAGAACCTGT GCTCAACGGC 
GGAGGCTACC CCTATGAGGC TGCCAACCGG GTGATTCCTG TCCACAGCAG TCACTCGGCC 
CCCATTCGAC CCTCCTACTC TGCTCAGGAG GGTTTAGCTG GCTACCAGCG TGAGGGACCC 
CACCCAGCCT GGTCACAGCA AGTGACCAGT GCCCACTGTG GCTGTGACCC CAGTGGTCTA 
TTCAGATCCC AGTCCTTTCC AGATGTGGAG CCCCAGCTGC CCCAAGCTCC AACCCGTGGG 
GGTAGTAGCC GAGAGGCTGT GCAGAGGGGT CTGAATTCAT GGCAACAGCA GCAGCCCCAC 
CCACCTCCCC GCCAGCAGGA GAGATCCCCA CTGCAGAGTC TTGCCCGCAG CAAGCCTAGC 
CCCCAGCTGT CAGCAGAGAC CCCCGTAGCT GCTCTCCCAG AGTTCCCAAG GGCAGCGTCC 
CAGCAGGAGA TCGAGCAGTC CATCGAAACC CTCAATATGT TGATGCTGGA CCTGGAGCCA 
GCCTCCGCAG CTGCCCCTCT TCATAAGTCC CAGAGTGTCC CTGGGGCCTG GCCAGGGGCT 
TCTCCACTCT CTTCCCAGCC CCTCTTGGGG TCTTCCCGCC AGTCCCACCC ACTTACCCAG 
TCCCGATCTG GCTATATCCC CAGTGGGCAT TCTCTGGGAA CCCCGGAGCT GGTCTCTTCT 
GGCAGGCCTT ATTCACCTTA CGATTATCAG CTGCATCCAG CTGGATCCAA CCAGAGTTTC 
CATCCAAAAA GTCCAGCCTC TTCCACCTTC CTTCCAAGCC CTCATAGCTC TGCGGGGCCT 
CAGGAGCCCC CAGCCTCTCT CCCTGGTCTC ATCGCTCAGC CCCAGCTCCC ACCAAAGGAA 
ACTACATCAG ATCCCTCCCG AACTCCAGAG GAAGAGCCGC TGAACCTGGA AGGGCTGGTA 
GCCCATCGGG TAGCAGGGGT TCAGGCTCGG GAGAGGCAGC CTGCAGAGCC CCCAGGCCCA 
CTCCGGAGGC GGGCAGCCAG TGATGGACAG TATGAAAACC AGTCTCCGGA GGCCACATCC 
CCCCGTAGTC CTGGGGTCCG CTCCCCCGTC CAGTGCGTCT CCCCTGAGCT GGCTCTTACC 
ATTGCCCTCA ATCCTGGAGG GCGCCCCAAA GAGCCTCATT TGCATAGCTA CAAGGAGGCC 
TTTGAGGAGA TGGAGGGGAC CTCCCCAAGC AGCCCGCCAC ACAGTGTGGC GCGCTCTCCT 
CCAGGTCTGG CCAAGACACC CCTGTCTGCT CTGGGCCTGA AACCCCACAA CCCAGCAGAC 
ATCTTGCTGC ACCCCACAGG TGTTGCCAGA AGACTGATCC AGCCAGAGGA AGACGAGGGG 
GAGGAGGTGA CTAAGCCTCC AGAAGAGCCC CGGAGCTATG TGGAGTCCGT GGCGAGGACA 
GCGGTGGCAG GGCCTCGAGC TCAGGACGTT GAGCCTAAGA GCTTCAGCGC ACCAGCTGCT 
CACGCCTATG GGCACGAGAC ACCCCTGAGG AACGGGACCC CAGGGGGCTC CTTTGTCTCC 
CCGAGCCCCC TCTCCACAAG CAGCCCCATC CTCAGTGCTG ACAGCACTTC CGTGGGCAGT 
TTCCCATCAG TGGTGAGCAG TGACCAGGGT CCCCGGACAC CCTTCCAGCC TATGCTGGAC 
TCCAGCATCC GCTCAGGCAG CTTGGGGCAG CCGAGCCCTG CAGCACTGAG TTATCAGAGC 
TCCTCGCCTG TCCCTGTTGG TGGCAGCAGC TACAACAGCC CTGACTACTC CCTCCAACCA 
TTCAGCTCTT CTCCAGAAAG CCAAGGCCAG CCACAGTACA GTGCGGCCAG TGTCCACATG 
GTACCTGGGA GCCCTCAGGC ACGACACAGG ACAGTGGGTA CCAACACTCC ACCTAGCCCT 
GGTTTTGGCC GGCGGGCCGT CAACCCCACC ATGGCTGCCC CTGGTAGTCC CAGTTTGAGC 
CATCGGCAGG TGATGGGTCC ATCTGGTCCA GGTTTCCATG GTAACGTGGT TTCTGGCCAC 
CCAGCCAGTG CAGCTACCAC TCCTGGAAGC CCCAGCCTGG GCCGGCACCC AGTGGGAAGC 
CATCAAGTTC CAGGCCTCCA CAGCAGTGTG GTCACCACTC CGGGAAGCCC AAGCCTGGGC 
CGACACCCTG GTGCCCACCA AGGGAATCTG GCCTCCAGTC TCCACAGCAA TGCAGTCATC 
AGCCCTGGGA GCCCAAGCTT GGGCCGGCAC CTGGGTGGGT CTGGATCCGT GGTTCCTGGA 
AGCCCCAGCT TGGACCGGCA TGCAGCTTAT GGTGGCTACT CTACCCCTGA GGACCGAAGA 
CCAACACTGT CCCGGCAAAG CAGTGCCTCT GGCTACCAGG CCCCATCCAC ACCGTCATTC 
CCTGTCTCTC CTGCCTACTA CCCTGGCCTG AGCAGCCCTG CCACCTCACC CTCGCCAGAC 
TCAGCAGCCT TCCGGCAGGG GAGCCCCACA CCAGCCTTAC CAGAGAAGCG AAGAATGTCA 
GTGGGAGACC GGGCAGGCAG CCTCCCCAAC TATGCCACCA TCAATGGGAA AGTGTCTTCC 
TCGCCTGTCG CCAATGGCAT GGCCAGTGGG AGCAGCACCG TCTCCTTCTC TCACACGCTT 
CCGGACTTTT CTAAGTATTC GATGCCAGAC AATAGTCCGG AGACTCGGGC TAAAGTGAAG 
TTTGTCCAGG ACACTTCCAA GTATTGGTAC AAGCCTGAGA TCTCCAGAGA ACAGGCCATC 
GCACTCCTAA AGGACCAGGA GCCAGGAGCC TTCATCATCC GGGACAGCCA CTCCTTCCGA 
GGGGCTTATG GGCTGGCCAT GAAGGTGTCC TCACCCCCTC CAACCATCAC TCAGCAGGGC 
AAGAAAGGAG ACATGACCCA TGAGCTGGTG AGGCACTTTC TGATCGAGAC AGGCCCTAGA 
GGAGTGAAGC TCAAGGGTTG TCCCAATGAG CCAAACTTTG GTTCCCTTTC TGCCCTGGTC 
TATCAGCACT CCGTCATCCC ACTGGCCCTG CCCTGTAAAC TGGTCATTCC AAGCCGAGAC 
CCCACAGATG AATCAAAAGA TAGCTCAGGC CCTGCCAACT CAACCACTGA CCTGCTGAAG 
CAAGGAGCAG CTTGCAATGT GCTCTTCGTC AACTCTGTGG ATATGGAGTC ACTCACCGGG 
CCACAGGCCA TTTCCAAAGC CACATCTGAG ACTCTGGCTG CCGACCCCAC ACCGGCTGCC 
ACCATTGTTC ACTTCAAGGT CTCTGCCCAA GGAATCACAC TGACTGACAA TCAAAGAAAG 
CTCTTCTTTA GAAGACACTA CCCCCTCAAC ACTGTCACCT TCTGTGACCT GGATCCACAA 
GAAAGAAAGT GGATGAAGAC AGAGGGAGGC GCCCCTGCTA AGCTCTTTGG CTTCGTGGCC 
CGGAAGCAGG GCAGCACCAC AGACAACGCT TGCCACCTCT TCGCAGAGCT TGACCCCAAC 
CAGCCTGCGT CTGCCATCGT CAACTTCGTC TCCAAGGTCA TGCTGAGTGC TGGCCAGAAG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108846.1
CDS	454..4239
Translation	MYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_030252986.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252986.1

ORF Insert Sequence:

ATGTACCGGG CTCAGCAACT CAGATCCCAT CCAGGCGGGG GCCCGACTGT GCCCTCCCCT 
GGCCGCCACA TTGTCCCAGC CCAGGTTCAC GTCAATGGTG GGGCCCTGGC ATCTGAGCGG 
GAGACAGACA TTCTAGATGA TGAGCTGCCC ATCCAGGACG GGCAGAGTGG GGGCAGCATG 
GGAACGCTGT CCTCCCTGGA TGGGGTCACC AACACCAGTG AAAGTGGCTA CCCAGAGACC 
CTGTCCCCAC TGACCAATGG CCTGGACAAA CCCTATTCCA CAGAACCTGT GCTCAACGGC 
GGAGGCTACC CCTATGAGGC TGCCAACCGG GTGATTCCTG TCCACAGCAG TCACTCGGCC 
CCCATTCGAC CCTCCTACTC TGCTCAGGAG GGTTTAGCTG GCTACCAGCG TGAGGGACCC 
CACCCAGCCT GGTCACAGCA AGTGACCAGT GCCCACTGTG GCTGTGACCC CAGTGGTCTA 
TTCAGATCCC AGTCCTTTCC AGATGTGGAG CCCCAGCTGC CCCAAGCTCC AACCCGTGGG 
GGTAGTAGCC GAGAGGCTGT GCAGAGGGGT CTGAATTCAT GGCAACAGCA GCAGCCCCAC 
CCACCTCCCC GCCAGCAGGA GAGATCCCCA CTGCAGAGTC TTGCCCGCAG CAAGCCTAGC 
CCCCAGCTGT CAGCAGAGAC CCCCGTAGCT GCTCTCCCAG AGTTCCCAAG GGCAGCGTCC 
CAGCAGGAGA TCGAGCAGTC CATCGAAACC CTCAATATGT TGATGCTGGA CCTGGAGCCA 
GCCTCCGCAG CTGCCCCTCT TCATAAGTCC CAGAGTGTCC CTGGGGCCTG GCCAGGGGCT 
TCTCCACTCT CTTCCCAGCC CCTCTTGGGG TCTTCCCGCC AGTCCCACCC ACTTACCCAG 
TCCCGATCTG GCTATATCCC CAGTGGGCAT TCTCTGGGAA CCCCGGAGCT GGTCTCTTCT 
GGCAGGCCTT ATTCACCTTA CGATTATCAG CTGCATCCAG CTGGATCCAA CCAGAGTTTC 
CATCCAAAAA GTCCAGCCTC TTCCACCTTC CTTCCAAGCC CTCATAGCTC TGCGGGGCCT 
CAGGAGCCCC CAGCCTCTCT CCCTGGTCTC ATCGCTCAGC CCCAGCTCCC ACCAAAGGAA 
ACTACATCAG ATCCCTCCCG AACTCCAGAG GAAGAGCCGC TGAACCTGGA AGGGCTGGTA 
GCCCATCGGG TAGCAGGGGT TCAGGCTCGG GAGAGGCAGC CTGCAGAGCC CCCAGGCCCA 
CTCCGGAGGC GGGCAGCCAG TGATGGACAG TATGAAAACC AGTCTCCGGA GGCCACATCC 
CCCCGTAGTC CTGGGGTCCG CTCCCCCGTC CAGTGCGTCT CCCCTGAGCT GGCTCTTACC 
ATTGCCCTCA ATCCTGGAGG GCGCCCCAAA GAGCCTCATT TGCATAGCTA CAAGGAGGCC 
TTTGAGGAGA TGGAGGGGAC CTCCCCAAGC AGCCCGCCAC ACAGTGTGGC GCGCTCTCCT 
CCAGGTCTGG CCAAGACACC CCTGTCTGCT CTGGGCCTGA AACCCCACAA CCCAGCAGAC 
ATCTTGCTGC ACCCCACAGG TGTTGCCAGA AGACTGATCC AGCCAGAGGA AGACGAGGGG 
GAGGAGGTGA CTAAGCCTCC AGAAGAGCCC CGGAGCTATG TGGAGTCCGT GGCGAGGACA 
GCGGTGGCAG GGCCTCGAGC TCAGGACGTT GAGCCTAAGA GCTTCAGCGC ACCAGCTGCT 
CACGCCTATG GGCACGAGAC ACCCCTGAGG AACGGGACCC CAGGGGGCTC CTTTGTCTCC 
CCGAGCCCCC TCTCCACAAG CAGCCCCATC CTCAGTGCTG ACAGCACTTC CGTGGGCAGT 
TTCCCATCAG TGGTGAGCAG TGACCAGGGT CCCCGGACAC CCTTCCAGCC TATGCTGGAC 
TCCAGCATCC GCTCAGGCAG CTTGGGGCAG CCGAGCCCTG CAGCACTGAG TTATCAGAGC 
TCCTCGCCTG TCCCTGTTGG TGGCAGCAGC TACAACAGCC CTGACTACTC CCTCCAACCA 
TTCAGCTCTT CTCCAGAAAG CCAAGGCCAG CCACAGTACA GTGCGGCCAG TGTCCACATG 
GTACCTGGGA GCCCTCAGGC ACGACACAGG ACAGTGGGTA CCAACACTCC ACCTAGCCCT 
GGTTTTGGCC GGCGGGCCGT CAACCCCACC ATGGCTGCCC CTGGTAGTCC CAGTTTGAGC 
CATCGGCAGG TGATGGGTCC ATCTGGTCCA GGTTTCCATG GTAACGTGGT TTCTGGCCAC 
CCAGCCAGTG CAGCTACCAC TCCTGGAAGC CCCAGCCTGG GCCGGCACCC AGTGGGAAGC 
CATCAAGTTC CAGGCCTCCA CAGCAGTGTG GTCACCACTC CGGGAAGCCC AAGCCTGGGC 
CGACACCCTG GTGCCCACCA AGGGAATCTG GCCTCCAGTC TCCACAGCAA TGCAGTCATC 
AGCCCTGGGA GCCCAAGCTT GGGCCGGCAC CTGGGTGGGT CTGGATCCGT GGTTCCTGGA 
AGCCCCAGCT TGGACCGGCA TGCAGCTTAT GGTGGCTACT CTACCCCTGA GGACCGAAGA 
CCAACACTGT CCCGGCAAAG CAGTGCCTCT GGCTACCAGG CCCCATCCAC ACCGTCATTC 
CCTGTCTCTC CTGCCTACTA CCCTGGCCTG AGCAGCCCTG CCACCTCACC CTCGCCAGAC 
TCAGCAGCCT TCCGGCAGGG GAGCCCCACA CCAGCCTTAC CAGAGAAGCG AAGAATGTCA 
GTGGGAGACC GGGCAGGCAG CCTCCCCAAC TATGCCACCA TCAATGGGAA AGTGTCTTCC 
TCGCCTGTCG CCAATGGCAT GGCCAGTGGG AGCAGCACCG TCTCCTTCTC TCACACGCTT 
CCGGACTTTT CTAAGTATTC GATGCCAGAC AATAGTCCGG AGACTCGGGC TAAAGTGAAG 
TTTGTCCAGG ACACTTCCAA GTATTGGTAC AAGCCTGAGA TCTCCAGAGA ACAGGCCATC 
GCACTCCTAA AGGACCAGGA GCCAGGAGCC TTCATCATCC GGGACAGCCA CTCCTTCCGA 
GGGGCTTATG GGCTGGCCAT GAAGGTGTCC TCACCCCCTC CAACCATCAC TCAGCAGGGC 
AAGAAAGGAG ACATGACCCA TGAGCTGGTG AGGCACTTTC TGATCGAGAC AGGCCCTAGA 
GGAGTGAAGC TCAAGGGTTG TCCCAATGAG CCAAACTTTG GTTCCCTTTC TGCCCTGGTC 
TATCAGCACT CCGTCATCCC ACTGGCCCTG CCCTGTAAAC TGGTCATTCC AAGCCGAGAC 
CCCACAGATG AATCAAAAGA TAGCTCAGGC CCTGCCAACT CAACCACTGA CCTGCTGAAG 
CAAGGAGCAG CTTGCAATGT GCTCTTCGTC AACTCTGTGG ATATGGAGTC ACTCACCGGG 
CCACAGGCCA TTTCCAAAGC CACATCTGAG ACTCTGGCTG CCGACCCCAC ACCGGCTGCC 
ACCATTGTTC ACTTCAAGGT CTCTGCCCAA GGAATCACAC TGACTGACAA TCAAAGAAAG 
CTCTTCTTTA GAAGACACTA CCCCCTCAAC ACTGTCACCT TCTGTGACCT GGATCCACAA 
GAAAGAAAGT GGATGAAGAC AGAGGGAGGC GCCCCTGCTA AGCTCTTTGG CTTCGTGGCC 
CGGAAGCAGG GCAGCACCAC AGACAACGCT TGCCACCTCT TCGCAGAGCT TGACCCCAAC 
CAGCCTGCGT CTGCCATCGT CAACTTCGTC TCCAAGGTCA TGCTGAGTGC TGGCCAGAAG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_030252987.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108847.1 Mus musculus tensin 1 (Tns1), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OMu85109
Clone ID Related Accession (Same CDS sequence)	XM_030252987.1
Accession Version	XM_030252987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCGGG CTCAGCAACT CAGATCCCAT CCAGGCGGGG GCCCGACTGT GCCCTCCCCT 
GGCCGCCACA TTGTCCCAGC CCAGGTTCAC GTCAATGGTG GGGCCCTGGC ATCTGAGCGG 
GAGACAGACA TTCTAGATGA TGAGCTGCCC ATCCAGGACG GGCAGAGTGG GGGCAGCATG 
GGAACGCTGT CCTCCCTGGA TGGGGTCACC AACACCAGTG AAAGTGGCTA CCCAGAGACC 
CTGTCCCCAC TGACCAATGG CCTGGACAAA CCCTATTCCA CAGAACCTGT GCTCAACGGC 
GGAGGCTACC CCTATGAGGC TGCCAACCGG GTGATTCCTG TCCACAGCAG TCACTCGGCC 
CCCATTCGAC CCTCCTACTC TGCTCAGGAG GGTTTAGCTG GCTACCAGCG TGAGGGACCC 
CACCCAGCCT GGTCACAGCA AGTGACCAGT GCCCACTGTG GCTGTGACCC CAGTGGTCTA 
TTCAGATCCC AGTCCTTTCC AGATGTGGAG CCCCAGCTGC CCCAAGCTCC AACCCGTGGG 
GGTAGTAGCC GAGAGGCTGT GCAGAGGGGT CTGAATTCAT GGCAACAGCA GCAGCCCCAC 
CCACCTCCCC GCCAGCAGGA GAGATCCCCA CTGCAGAGTC TTGCCCGCAG CAAGCCTAGC 
CCCCAGCTGT CAGCAGAGAC CCCCGTAGCT GCTCTCCCAG AGTTCCCAAG GGCAGCGTCC 
CAGCAGGAGA TCGAGCAGTC CATCGAAACC CTCAATATGT TGATGCTGGA CCTGGAGCCA 
GCCTCCGCAG CTGCCCCTCT TCATAAGTCC CAGAGTGTCC CTGGGGCCTG GCCAGGGGCT 
TCTCCACTCT CTTCCCAGCC CCTCTTGGGG TCTTCCCGCC AGTCCCACCC ACTTACCCAG 
TCCCGATCTG GCTATATCCC CAGTGGGCAT TCTCTGGGAA CCCCGGAGCT GGTCTCTTCT 
GGCAGGCCTT ATTCACCTTA CGATTATCAG CTGCATCCAG CTGGATCCAA CCAGAGTTTC 
CATCCAAAAA GTCCAGCCTC TTCCACCTTC CTTCCAAGCC CTCATAGCTC TGCGGGGCCT 
CAGGAGCCCC CAGCCTCTCT CCCTGGTCTC ATCGCTCAGC CCCAGCTCCC ACCAAAGGAA 
ACTACATCAG ATCCCTCCCG AACTCCAGAG GAAGAGCCGC TGAACCTGGA AGGGCTGGTA 
GCCCATCGGG TAGCAGGGGT TCAGGCTCGG GAGAGGCAGC CTGCAGAGCC CCCAGGCCCA 
CTCCGGAGGC GGGCAGCCAG TGATGGACAG TATGAAAACC AGTCTCCGGA GGCCACATCC 
CCCCGTAGTC CTGGGGTCCG CTCCCCCGTC CAGTGCGTCT CCCCTGAGCT GGCTCTTACC 
ATTGCCCTCA ATCCTGGAGG GCGCCCCAAA GAGCCTCATT TGCATAGCTA CAAGGAGGCC 
TTTGAGGAGA TGGAGGGGAC CTCCCCAAGC AGCCCGCCAC ACAGTGTGGC GCGCTCTCCT 
CCAGGTCTGG CCAAGACACC CCTGTCTGCT CTGGGCCTGA AACCCCACAA CCCAGCAGAC 
ATCTTGCTGC ACCCCACAGG TGAGCCCCGG AGCTATGTGG AGTCCGTGGC GAGGACAGCG 
GTGGCAGGGC CTCGAGCTCA GGACGTTGAG CCTAAGAGCT TCAGCGCACC AGCTGCTCAC 
GCCTATGGGC ACGAGACACC CCTGAGGAAC GGGACCCCAG GGGGCTCCTT TGTCTCCCCG 
AGCCCCCTCT CCACAAGCAG CCCCATCCTC AGTGCTGACA GCACTTCCGT GGGCAGTTTC 
CCATCAGTGG TGAGCAGTGA CCAGGGTCCC CGGACACCCT TCCAGCCTAT GCTGGACTCC 
AGCATCCGCT CAGGCAGCTT GGGGCAGCCG AGCCCTGCAG CACTGAGTTA TCAGAGCTCC 
TCGCCTGTCC CTGTTGGTGG CAGCAGCTAC AACAGCCCTG ACTACTCCCT CCAACCATTC 
AGCTCTTCTC CAGAAAGCCA AGGCCAGCCA CAGTACAGTG CGGCCAGTGT CCACATGGTA 
CCTGGGAGCC CTCAGGCACG ACACAGGACA GTGGGTACCA ACACTCCACC TAGCCCTGGT 
TTTGGCCGGC GGGCCGTCAA CCCCACCATG GCTGCCCCTG GTAGTCCCAG TTTGAGCCAT 
CGGCAGGTGA TGGGTCCATC TGGTCCAGGT TTCCATGGTA ACGTGGTTTC TGGCCACCCA 
GCCAGTGCAG CTACCACTCC TGGAAGCCCC AGCCTGGGCC GGCACCCAGT GGGAAGCCAT 
CAAGTTCCAG GCCTCCACAG CAGTGTGGTC ACCACTCCGG GAAGCCCAAG CCTGGGCCGA 
CACCCTGGTG CCCACCAAGG GAATCTGGCC TCCAGTCTCC ACAGCAATGC AGTCATCAGC 
CCTGGGAGCC CAAGCTTGGG CCGGCACCTG GGTGGGTCTG GATCCGTGGT TCCTGGAAGC 
CCCAGCTTGG ACCGGCATGC AGCTTATGGT GGCTACTCTA CCCCTGAGGA CCGAAGACCA 
ACACTGTCCC GGCAAAGCAG TGCCTCTGGC TACCAGGCCC CATCCACACC GTCATTCCCT 
GTCTCTCCTG CCTACTACCC TGGCCTGAGC AGCCCTGCCA CCTCACCCTC GCCAGACTCA 
GCAGCCTTCC GGCAGGGGAG CCCCACACCA GCCTTACCAG AGAAGCGAAG AATGTCAGTG 
GGAGACCGGG CAGGCAGCCT CCCCAACTAT GCCACCATCA ATGGGAAAGT GTCTTCCTCG 
CCTGTCGCCA ATGGCATGGC CAGTGGGAGC AGCACCGTCT CCTTCTCTCA CACGCTTCCG 
GACTTTTCTA AGTATTCGAT GCCAGACAAT AGTCCGGAGA CTCGGGCTAA AGTGAAGTTT 
GTCCAGGACA CTTCCAAGTA TTGGTACAAG CCTGAGATCT CCAGAGAACA GGCCATCGCA 
CTCCTAAAGG ACCAGGAGCC AGGAGCCTTC ATCATCCGGG ACAGCCACTC CTTCCGAGGG 
GCTTATGGGC TGGCCATGAA GGTGTCCTCA CCCCCTCCAA CCATCACTCA GCAGGGCAAG 
AAAGGAGACA TGACCCATGA GCTGGTGAGG CACTTTCTGA TCGAGACAGG CCCTAGAGGA 
GTGAAGCTCA AGGGTTGTCC CAATGAGCCA AACTTTGGTT CCCTTTCTGC CCTGGTCTAT 
CAGCACTCCG TCATCCCACT GGCCCTGCCC TGTAAACTGG TCATTCCAAG CCGAGACCCC 
ACAGATGAAT CAAAAGATAG CTCAGGCCCT GCCAACTCAA CCACTGACCT GCTGAAGCAA 
GGAGCAGCTT GCAATGTGCT CTTCGTCAAC TCTGTGGATA TGGAGTCACT CACCGGGCCA 
CAGGCCATTT CCAAAGCCAC ATCTGAGACT CTGGCTGCCG ACCCCACACC GGCTGCCACC 
ATTGTTCACT TCAAGGTCTC TGCCCAAGGA ATCACACTGA CTGACAATCA AAGAAAGCTC 
TTCTTTAGAA GACACTACCC CCTCAACACT GTCACCTTCT GTGACCTGGA TCCACAAGAA 
AGAAAGTGGA TGAAGACAGA GGGAGGCGCC CCTGCTAAGC TCTTTGGCTT CGTGGCCCGG 
AAGCAGGGCA GCACCACAGA CAACGCTTGC CACCTCTTCG CAGAGCTTGA CCCCAACCAG 
CCTGCGTCTG CCATCGTCAA CTTCGTCTCC AAGGTCATGC TGAGTGCTGG CCAGAAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108847.1
CDS	454..4176
Translation	MYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_030252987.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252987.1

ORF Insert Sequence:

ATGTACCGGG CTCAGCAACT CAGATCCCAT CCAGGCGGGG GCCCGACTGT GCCCTCCCCT 
GGCCGCCACA TTGTCCCAGC CCAGGTTCAC GTCAATGGTG GGGCCCTGGC ATCTGAGCGG 
GAGACAGACA TTCTAGATGA TGAGCTGCCC ATCCAGGACG GGCAGAGTGG GGGCAGCATG 
GGAACGCTGT CCTCCCTGGA TGGGGTCACC AACACCAGTG AAAGTGGCTA CCCAGAGACC 
CTGTCCCCAC TGACCAATGG CCTGGACAAA CCCTATTCCA CAGAACCTGT GCTCAACGGC 
GGAGGCTACC CCTATGAGGC TGCCAACCGG GTGATTCCTG TCCACAGCAG TCACTCGGCC 
CCCATTCGAC CCTCCTACTC TGCTCAGGAG GGTTTAGCTG GCTACCAGCG TGAGGGACCC 
CACCCAGCCT GGTCACAGCA AGTGACCAGT GCCCACTGTG GCTGTGACCC CAGTGGTCTA 
TTCAGATCCC AGTCCTTTCC AGATGTGGAG CCCCAGCTGC CCCAAGCTCC AACCCGTGGG 
GGTAGTAGCC GAGAGGCTGT GCAGAGGGGT CTGAATTCAT GGCAACAGCA GCAGCCCCAC 
CCACCTCCCC GCCAGCAGGA GAGATCCCCA CTGCAGAGTC TTGCCCGCAG CAAGCCTAGC 
CCCCAGCTGT CAGCAGAGAC CCCCGTAGCT GCTCTCCCAG AGTTCCCAAG GGCAGCGTCC 
CAGCAGGAGA TCGAGCAGTC CATCGAAACC CTCAATATGT TGATGCTGGA CCTGGAGCCA 
GCCTCCGCAG CTGCCCCTCT TCATAAGTCC CAGAGTGTCC CTGGGGCCTG GCCAGGGGCT 
TCTCCACTCT CTTCCCAGCC CCTCTTGGGG TCTTCCCGCC AGTCCCACCC ACTTACCCAG 
TCCCGATCTG GCTATATCCC CAGTGGGCAT TCTCTGGGAA CCCCGGAGCT GGTCTCTTCT 
GGCAGGCCTT ATTCACCTTA CGATTATCAG CTGCATCCAG CTGGATCCAA CCAGAGTTTC 
CATCCAAAAA GTCCAGCCTC TTCCACCTTC CTTCCAAGCC CTCATAGCTC TGCGGGGCCT 
CAGGAGCCCC CAGCCTCTCT CCCTGGTCTC ATCGCTCAGC CCCAGCTCCC ACCAAAGGAA 
ACTACATCAG ATCCCTCCCG AACTCCAGAG GAAGAGCCGC TGAACCTGGA AGGGCTGGTA 
GCCCATCGGG TAGCAGGGGT TCAGGCTCGG GAGAGGCAGC CTGCAGAGCC CCCAGGCCCA 
CTCCGGAGGC GGGCAGCCAG TGATGGACAG TATGAAAACC AGTCTCCGGA GGCCACATCC 
CCCCGTAGTC CTGGGGTCCG CTCCCCCGTC CAGTGCGTCT CCCCTGAGCT GGCTCTTACC 
ATTGCCCTCA ATCCTGGAGG GCGCCCCAAA GAGCCTCATT TGCATAGCTA CAAGGAGGCC 
TTTGAGGAGA TGGAGGGGAC CTCCCCAAGC AGCCCGCCAC ACAGTGTGGC GCGCTCTCCT 
CCAGGTCTGG CCAAGACACC CCTGTCTGCT CTGGGCCTGA AACCCCACAA CCCAGCAGAC 
ATCTTGCTGC ACCCCACAGG TGAGCCCCGG AGCTATGTGG AGTCCGTGGC GAGGACAGCG 
GTGGCAGGGC CTCGAGCTCA GGACGTTGAG CCTAAGAGCT TCAGCGCACC AGCTGCTCAC 
GCCTATGGGC ACGAGACACC CCTGAGGAAC GGGACCCCAG GGGGCTCCTT TGTCTCCCCG 
AGCCCCCTCT CCACAAGCAG CCCCATCCTC AGTGCTGACA GCACTTCCGT GGGCAGTTTC 
CCATCAGTGG TGAGCAGTGA CCAGGGTCCC CGGACACCCT TCCAGCCTAT GCTGGACTCC 
AGCATCCGCT CAGGCAGCTT GGGGCAGCCG AGCCCTGCAG CACTGAGTTA TCAGAGCTCC 
TCGCCTGTCC CTGTTGGTGG CAGCAGCTAC AACAGCCCTG ACTACTCCCT CCAACCATTC 
AGCTCTTCTC CAGAAAGCCA AGGCCAGCCA CAGTACAGTG CGGCCAGTGT CCACATGGTA 
CCTGGGAGCC CTCAGGCACG ACACAGGACA GTGGGTACCA ACACTCCACC TAGCCCTGGT 
TTTGGCCGGC GGGCCGTCAA CCCCACCATG GCTGCCCCTG GTAGTCCCAG TTTGAGCCAT 
CGGCAGGTGA TGGGTCCATC TGGTCCAGGT TTCCATGGTA ACGTGGTTTC TGGCCACCCA 
GCCAGTGCAG CTACCACTCC TGGAAGCCCC AGCCTGGGCC GGCACCCAGT GGGAAGCCAT 
CAAGTTCCAG GCCTCCACAG CAGTGTGGTC ACCACTCCGG GAAGCCCAAG CCTGGGCCGA 
CACCCTGGTG CCCACCAAGG GAATCTGGCC TCCAGTCTCC ACAGCAATGC AGTCATCAGC 
CCTGGGAGCC CAAGCTTGGG CCGGCACCTG GGTGGGTCTG GATCCGTGGT TCCTGGAAGC 
CCCAGCTTGG ACCGGCATGC AGCTTATGGT GGCTACTCTA CCCCTGAGGA CCGAAGACCA 
ACACTGTCCC GGCAAAGCAG TGCCTCTGGC TACCAGGCCC CATCCACACC GTCATTCCCT 
GTCTCTCCTG CCTACTACCC TGGCCTGAGC AGCCCTGCCA CCTCACCCTC GCCAGACTCA 
GCAGCCTTCC GGCAGGGGAG CCCCACACCA GCCTTACCAG AGAAGCGAAG AATGTCAGTG 
GGAGACCGGG CAGGCAGCCT CCCCAACTAT GCCACCATCA ATGGGAAAGT GTCTTCCTCG 
CCTGTCGCCA ATGGCATGGC CAGTGGGAGC AGCACCGTCT CCTTCTCTCA CACGCTTCCG 
GACTTTTCTA AGTATTCGAT GCCAGACAAT AGTCCGGAGA CTCGGGCTAA AGTGAAGTTT 
GTCCAGGACA CTTCCAAGTA TTGGTACAAG CCTGAGATCT CCAGAGAACA GGCCATCGCA 
CTCCTAAAGG ACCAGGAGCC AGGAGCCTTC ATCATCCGGG ACAGCCACTC CTTCCGAGGG 
GCTTATGGGC TGGCCATGAA GGTGTCCTCA CCCCCTCCAA CCATCACTCA GCAGGGCAAG 
AAAGGAGACA TGACCCATGA GCTGGTGAGG CACTTTCTGA TCGAGACAGG CCCTAGAGGA 
GTGAAGCTCA AGGGTTGTCC CAATGAGCCA AACTTTGGTT CCCTTTCTGC CCTGGTCTAT 
CAGCACTCCG TCATCCCACT GGCCCTGCCC TGTAAACTGG TCATTCCAAG CCGAGACCCC 
ACAGATGAAT CAAAAGATAG CTCAGGCCCT GCCAACTCAA CCACTGACCT GCTGAAGCAA 
GGAGCAGCTT GCAATGTGCT CTTCGTCAAC TCTGTGGATA TGGAGTCACT CACCGGGCCA 
CAGGCCATTT CCAAAGCCAC ATCTGAGACT CTGGCTGCCG ACCCCACACC GGCTGCCACC 
ATTGTTCACT TCAAGGTCTC TGCCCAAGGA ATCACACTGA CTGACAATCA AAGAAAGCTC 
TTCTTTAGAA GACACTACCC CCTCAACACT GTCACCTTCT GTGACCTGGA TCCACAAGAA 
AGAAAGTGGA TGAAGACAGA GGGAGGCGCC CCTGCTAAGC TCTTTGGCTT CGTGGCCCGG 
AAGCAGGGCA GCACCACAGA CAACGCTTGC CACCTCTTCG CAGAGCTTGA CCCCAACCAG 
CCTGCGTCTG CCATCGTCAA CTTCGTCTCC AAGGTCATGC TGAGTGCTGG CCAGAAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_030252988.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108848.1 Mus musculus tensin 1 (Tns1), transcript variant X34, mRNA.

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CloneID	OMu85110
Clone ID Related Accession (Same CDS sequence)	XM_030252988.1
Accession Version	XM_030252988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCGGG CTCAGCAACT CAGATCCCAT CCAGGCGGGG GCCCGACTGT GCCCTCCCCT 
GGCCGCCACA TTGTCCCAGC CCAGGTTCAC GTCAATGGTG GGGCCCTGGC ATCTGAGCGG 
GAGACAGACA TTCTAGATGA TGAGCTGCCC ATCCAGGACG GGCAGAGTGG GGGCAGCATG 
GGAACGCTGT CCTCCCTGGA TGGGGTCACC AACACCAGTG AAAGTGGCTA CCCAGAGACC 
CTGTCCCCAC TGACCAATGG CCTGGACAAA CCCTATTCCA CAGAACCTGT GCTCAACGGC 
GGAGGCTACC CCTATGAGGC TGCCAACCGG GTGATTCCTG TCCACAGCAG TCACTCGGCC 
CCCATTCGAC CCTCCTACTC TGCTCAGGAG GGTTTAGCTG GCTACCAGCG TGAGGGACCC 
CACCCAGCCT GGTCACAGCA AGTGACCAGT GCCCACTGTG GCTGTGACCC CAGTGGTCTA 
TTCAGATCCC AGTCCTTTCC AGATGTGGAG CCCCAGCTGC CCCAAGCTCC AACCCGTGGG 
GGTAGTAGCC GAGAGGCTGT GCAGAGGGGT CTGAATTCAT GGCAACAGCA GCAGCCCCAC 
CCACCTCCCC GCCAGCAGGA GAGATCCCCA CTGCAGAGTC TTGCCCGCAG CAAGCCTAGC 
CCCCAGCTGT CAGCAGAGAC CCCCGTAGCT GCTCTCCCAG AGTTCCCAAG GGCAGCGTCC 
CAGCAGGAGA TCGAGCAGTC CATCGAAACC CTCAATATGT TGATGCTGGA CCTGGAGCCA 
GCCTCCGCAG CTGCCCCTCT TCATAAGTCC CAGAGTGTCC CTGGGGCCTG GCCAGGGGCT 
TCTCCACTCT CTTCCCAGCC CCTCTTGGGG TCTTCCCGCC AGTCCCACCC ACTTACCCAG 
TCCCGATCTG GCTATATCCC CAGTGGGCAT TCTCTGGGAA CCCCGGAGCT GGTCTCTTCT 
GGCAGGCCTT ATTCACCTTA CGATTATCAG CTGCATCCAG CTGGATCCAA CCAGAGTTTC 
CATCCAAAAA GTCCAGCCTC TTCCACCTTC CTTCCAAGCC CTCATAGCTC TGCGGGGCCT 
CAGGAGCCCC CAGCCTCTCT CCCTGGTCTC ATCGCTCAGC CCCAGCTCCC ACCAAAGGAA 
ACTACATCAG ATCCCTCCCG AACTCCAGAG GAAGAGCCGC TGAACCTGGA AGGGCTGGTA 
GCCCATCGGG TAGCAGAGCC CCGGAGCTAT GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA 
GGGCCTCGAG CTCAGGACGT TGAGCCTAAG AGCTTCAGCG CACCAGCTGC TCACGCCTAT 
GGGCACGAGA CACCCCTGAG GAACGGGACC CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC 
CTCTCCACAA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CCGTGGGCAG TTTCCCATCA 
GTGGTGAGCA GTGACCAGGG TCCCCGGACA CCCTTCCAGC CTATGCTGGA CTCCAGCATC 
CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT GCAGCACTGA GTTATCAGAG CTCCTCGCCT 
GTCCCTGTTG GTGGCAGCAG CTACAACAGC CCTGACTACT CCCTCCAACC ATTCAGCTCT 
TCTCCAGAAA GCCAAGGCCA GCCACAGTAC AGTGCGGCCA GTGTCCACAT GGTACCTGGG 
AGCCCTCAGG CACGACACAG GACAGTGGGT ACCAACACTC CACCTAGCCC TGGTTTTGGC 
CGGCGGGCCG TCAACCCCAC CATGGCTGCC CCTGGTAGTC CCAGTTTGAG CCATCGGCAG 
GTGATGGGTC CATCTGGTCC AGGTTTCCAT GGTAACGTGG TTTCTGGCCA CCCAGCCAGT 
GCAGCTACCA CTCCTGGAAG CCCCAGCCTG GGCCGGCACC CAGTGGGAAG CCATCAAGTT 
CCAGGCCTCC ACAGCAGTGT GGTCACCACT CCGGGAAGCC CAAGCCTGGG CCGACACCCT 
GGTGCCCACC AAGGGAATCT GGCCTCCAGT CTCCACAGCA ATGCAGTCAT CAGCCCTGGG 
AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG TCTGGATCCG TGGTTCCTGG AAGCCCCAGC 
TTGGACCGGC ATGCAGCTTA TGGTGGCTAC TCTACCCCTG AGGACCGAAG ACCAACACTG 
TCCCGGCAAA GCAGTGCCTC TGGCTACCAG GCCCCATCCA CACCGTCATT CCCTGTCTCT 
CCTGCCTACT ACCCTGGCCT GAGCAGCCCT GCCACCTCAC CCTCGCCAGA CTCAGCAGCC 
TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA CCAGAGAAGC GAAGAATGTC AGTGGGAGAC 
CGGGCAGGCA GCCTCCCCAA CTATGCCACC ATCAATGGGA AAGTGTCTTC CTCGCCTGTC 
GCCAATGGCA TGGCCAGTGG GAGCAGCACC GTCTCCTTCT CTCACACGCT TCCGGACTTT 
TCTAAGTATT CGATGCCAGA CAATAGTCCG GAGACTCGGG CTAAAGTGAA GTTTGTCCAG 
GACACTTCCA AGTATTGGTA CAAGCCTGAG ATCTCCAGAG AACAGGCCAT CGCACTCCTA 
AAGGACCAGG AGCCAGGAGC CTTCATCATC CGGGACAGCC ACTCCTTCCG AGGGGCTTAT 
GGGCTGGCCA TGAAGGTGTC CTCACCCCCT CCAACCATCA CTCAGCAGGG CAAGAAAGGA 
GACATGACCC ATGAGCTGGT GAGGCACTTT CTGATCGAGA CAGGCCCTAG AGGAGTGAAG 
CTCAAGGGTT GTCCCAATGA GCCAAACTTT GGTTCCCTTT CTGCCCTGGT CTATCAGCAC 
TCCGTCATCC CACTGGCCCT GCCCTGTAAA CTGGTCATTC CAAGCCGAGA CCCCACAGAT 
GAATCAAAAG ATAGCTCAGG CCCTGCCAAC TCAACCACTG ACCTGCTGAA GCAAGGAGCA 
GCTTGCAATG TGCTCTTCGT CAACTCTGTG GATATGGAGT CACTCACCGG GCCACAGGCC 
ATTTCCAAAG CCACATCTGA GACTCTGGCT GCCGACCCCA CACCGGCTGC CACCATTGTT 
CACTTCAAGG TCTCTGCCCA AGGAATCACA CTGACTGACA ATCAAAGAAA GCTCTTCTTT 
AGAAGACACT ACCCCCTCAA CACTGTCACC TTCTGTGACC TGGATCCACA AGAAAGAAAG 
TGGATGAAGA CAGAGGGAGG CGCCCCTGCT AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG 
GGCAGCACCA CAGACAACGC TTGCCACCTC TTCGCAGAGC TTGACCCCAA CCAGCCTGCG 
TCTGCCATCG TCAACTTCGT CTCCAAGGTC ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108848.1
CDS	454..3810
Translation	MYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_030252988.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252988.1

ORF Insert Sequence:

ATGTACCGGG CTCAGCAACT CAGATCCCAT CCAGGCGGGG GCCCGACTGT GCCCTCCCCT 
GGCCGCCACA TTGTCCCAGC CCAGGTTCAC GTCAATGGTG GGGCCCTGGC ATCTGAGCGG 
GAGACAGACA TTCTAGATGA TGAGCTGCCC ATCCAGGACG GGCAGAGTGG GGGCAGCATG 
GGAACGCTGT CCTCCCTGGA TGGGGTCACC AACACCAGTG AAAGTGGCTA CCCAGAGACC 
CTGTCCCCAC TGACCAATGG CCTGGACAAA CCCTATTCCA CAGAACCTGT GCTCAACGGC 
GGAGGCTACC CCTATGAGGC TGCCAACCGG GTGATTCCTG TCCACAGCAG TCACTCGGCC 
CCCATTCGAC CCTCCTACTC TGCTCAGGAG GGTTTAGCTG GCTACCAGCG TGAGGGACCC 
CACCCAGCCT GGTCACAGCA AGTGACCAGT GCCCACTGTG GCTGTGACCC CAGTGGTCTA 
TTCAGATCCC AGTCCTTTCC AGATGTGGAG CCCCAGCTGC CCCAAGCTCC AACCCGTGGG 
GGTAGTAGCC GAGAGGCTGT GCAGAGGGGT CTGAATTCAT GGCAACAGCA GCAGCCCCAC 
CCACCTCCCC GCCAGCAGGA GAGATCCCCA CTGCAGAGTC TTGCCCGCAG CAAGCCTAGC 
CCCCAGCTGT CAGCAGAGAC CCCCGTAGCT GCTCTCCCAG AGTTCCCAAG GGCAGCGTCC 
CAGCAGGAGA TCGAGCAGTC CATCGAAACC CTCAATATGT TGATGCTGGA CCTGGAGCCA 
GCCTCCGCAG CTGCCCCTCT TCATAAGTCC CAGAGTGTCC CTGGGGCCTG GCCAGGGGCT 
TCTCCACTCT CTTCCCAGCC CCTCTTGGGG TCTTCCCGCC AGTCCCACCC ACTTACCCAG 
TCCCGATCTG GCTATATCCC CAGTGGGCAT TCTCTGGGAA CCCCGGAGCT GGTCTCTTCT 
GGCAGGCCTT ATTCACCTTA CGATTATCAG CTGCATCCAG CTGGATCCAA CCAGAGTTTC 
CATCCAAAAA GTCCAGCCTC TTCCACCTTC CTTCCAAGCC CTCATAGCTC TGCGGGGCCT 
CAGGAGCCCC CAGCCTCTCT CCCTGGTCTC ATCGCTCAGC CCCAGCTCCC ACCAAAGGAA 
ACTACATCAG ATCCCTCCCG AACTCCAGAG GAAGAGCCGC TGAACCTGGA AGGGCTGGTA 
GCCCATCGGG TAGCAGAGCC CCGGAGCTAT GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA 
GGGCCTCGAG CTCAGGACGT TGAGCCTAAG AGCTTCAGCG CACCAGCTGC TCACGCCTAT 
GGGCACGAGA CACCCCTGAG GAACGGGACC CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC 
CTCTCCACAA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CCGTGGGCAG TTTCCCATCA 
GTGGTGAGCA GTGACCAGGG TCCCCGGACA CCCTTCCAGC CTATGCTGGA CTCCAGCATC 
CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT GCAGCACTGA GTTATCAGAG CTCCTCGCCT 
GTCCCTGTTG GTGGCAGCAG CTACAACAGC CCTGACTACT CCCTCCAACC ATTCAGCTCT 
TCTCCAGAAA GCCAAGGCCA GCCACAGTAC AGTGCGGCCA GTGTCCACAT GGTACCTGGG 
AGCCCTCAGG CACGACACAG GACAGTGGGT ACCAACACTC CACCTAGCCC TGGTTTTGGC 
CGGCGGGCCG TCAACCCCAC CATGGCTGCC CCTGGTAGTC CCAGTTTGAG CCATCGGCAG 
GTGATGGGTC CATCTGGTCC AGGTTTCCAT GGTAACGTGG TTTCTGGCCA CCCAGCCAGT 
GCAGCTACCA CTCCTGGAAG CCCCAGCCTG GGCCGGCACC CAGTGGGAAG CCATCAAGTT 
CCAGGCCTCC ACAGCAGTGT GGTCACCACT CCGGGAAGCC CAAGCCTGGG CCGACACCCT 
GGTGCCCACC AAGGGAATCT GGCCTCCAGT CTCCACAGCA ATGCAGTCAT CAGCCCTGGG 
AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG TCTGGATCCG TGGTTCCTGG AAGCCCCAGC 
TTGGACCGGC ATGCAGCTTA TGGTGGCTAC TCTACCCCTG AGGACCGAAG ACCAACACTG 
TCCCGGCAAA GCAGTGCCTC TGGCTACCAG GCCCCATCCA CACCGTCATT CCCTGTCTCT 
CCTGCCTACT ACCCTGGCCT GAGCAGCCCT GCCACCTCAC CCTCGCCAGA CTCAGCAGCC 
TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA CCAGAGAAGC GAAGAATGTC AGTGGGAGAC 
CGGGCAGGCA GCCTCCCCAA CTATGCCACC ATCAATGGGA AAGTGTCTTC CTCGCCTGTC 
GCCAATGGCA TGGCCAGTGG GAGCAGCACC GTCTCCTTCT CTCACACGCT TCCGGACTTT 
TCTAAGTATT CGATGCCAGA CAATAGTCCG GAGACTCGGG CTAAAGTGAA GTTTGTCCAG 
GACACTTCCA AGTATTGGTA CAAGCCTGAG ATCTCCAGAG AACAGGCCAT CGCACTCCTA 
AAGGACCAGG AGCCAGGAGC CTTCATCATC CGGGACAGCC ACTCCTTCCG AGGGGCTTAT 
GGGCTGGCCA TGAAGGTGTC CTCACCCCCT CCAACCATCA CTCAGCAGGG CAAGAAAGGA 
GACATGACCC ATGAGCTGGT GAGGCACTTT CTGATCGAGA CAGGCCCTAG AGGAGTGAAG 
CTCAAGGGTT GTCCCAATGA GCCAAACTTT GGTTCCCTTT CTGCCCTGGT CTATCAGCAC 
TCCGTCATCC CACTGGCCCT GCCCTGTAAA CTGGTCATTC CAAGCCGAGA CCCCACAGAT 
GAATCAAAAG ATAGCTCAGG CCCTGCCAAC TCAACCACTG ACCTGCTGAA GCAAGGAGCA 
GCTTGCAATG TGCTCTTCGT CAACTCTGTG GATATGGAGT CACTCACCGG GCCACAGGCC 
ATTTCCAAAG CCACATCTGA GACTCTGGCT GCCGACCCCA CACCGGCTGC CACCATTGTT 
CACTTCAAGG TCTCTGCCCA AGGAATCACA CTGACTGACA ATCAAAGAAA GCTCTTCTTT 
AGAAGACACT ACCCCCTCAA CACTGTCACC TTCTGTGACC TGGATCCACA AGAAAGAAAG 
TGGATGAAGA CAGAGGGAGG CGCCCCTGCT AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG 
GGCAGCACCA CAGACAACGC TTGCCACCTC TTCGCAGAGC TTGACCCCAA CCAGCCTGCG 
TCTGCCATCG TCAACTTCGT CTCCAAGGTC ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495883.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495946.1 Mus musculus tensin 1 (Tns1), transcript variant X27, mRNA.

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CloneID	OMu27366
Clone ID Related Accession (Same CDS sequence)	XM_006495883.1 , XM_006495883.2
Accession Version	XM_006495883.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCGGG CTCAGCAACT CAGATCCCAT CCAGGCGGGG GCCCGACTGT GCCCTCCCCT 
GGCCGCCACA TTGTCCCAGC CCAGGTTCAC GTCAATGGTG GGGCCCTGGC ATCTGAGCGG 
GAGACAGACA TTCTAGATGA TGAGCTGCCC ATCCAGGACG GGCAGAGTGG GGGCAGCATG 
GGAACGCTGT CCTCCCTGGA TGGGGTCACC AACACCAGTG AAAGTGGCTA CCCAGAGACC 
CTGTCCCCAC TGACCAATGG CCTGGACAAA CCCTATTCCA CAGAACCTGT GCTCAACGGC 
GGAGGCTACC CCTATGAGGC TGCCAACCGG GTGATTCCTG TCCACAGCAG TCACTCGGCC 
CCCATTCGAC CCTCCTACTC TGCTCAGGAG GGTTTAGCTG GCTACCAGCG TGAGGGACCC 
CACCCAGCCT GGTCACAGCA AGTGACCAGT GCCCACTGTG GCTGTGACCC CAGTGGTCTA 
TTCAGATCCC AGTCCTTTCC AGATGTGGAG CCCCAGCTGC CCCAAGCTCC AACCCGTGGG 
GGTAGTAGCC GAGAGGCTGT GCAGAGGGGT CTGAATTCAT GGCAACAGCA GCAGCCCCAC 
CCACCTCCCC GCCAGCAGGA GAGATCCCCA CTGCAGAGTC TTGCCCGCAG CAAGCCTAGC 
CCCCAGCTGT CAGCAGAGAC CCCCGTAGCT GCTCTCCCAG AGTTCCCAAG GGCAGCGTCC 
CAGCAGGAGA TCGAGCAGTC CATCGAAACC CTCAATATGT TGATGCTGGA CCTGGAGCCA 
GCCTCCGCAG CTGCCCCTCT TCATAAGTCC CAGAGTGTCC CTGGGGCCTG GCCAGGGGCT 
TCTCCACTCT CTTCCCAGCC CCTCTTGGGG TCTTCCCGCC AGTCCCACCC ACTTACCCAG 
TCCCGATCTG GCTATATCCC CAGTGGGCAT TCTCTGGGAA CCCCGGAGCT GGTCTCTTCT 
GGCAGGCCTT ATTCACCTTA CGATTATCAG CTGCATCCAG CTGGATCCAA CCAGAGTTTC 
CATCCAAAAA GTCCAGCCTC TTCCACCTTC CTTCCAAGCC CTCATAGCTC TGCGGGGCCT 
CAGGAGCCCC CAGCCTCTCT CCCTGGTCTC ATCGCTCAGC CCCAGCTCCC ACCAAAGGAA 
ACTACATCAG ATCCCTCCCG AACTCCAGAG GAAGAGCCGC TGAACCTGGA AGGGCTGGTA 
GCCCATCGGG TAGCAGTGTA CAATGCCAGA CTACAGAGCC TGAGGCAGAG CCGCGGCTTC 
CAACCCTCTC CCCACTGGCG CCGATCTGCC TCCCTCTCTG GGGTTCAGGC TCGGGAGAGG 
CAGCCTGCAG AGCCCCCAGG CCCACTCCGG AGGCGGGCAG CCAGTGATGG ACAGTATGAA 
AACCAGTCTC CGGAGGCCAC ATCCCCCCGT AGTCCTGGGG TCCGCTCCCC CGTCCAGTGC 
GTCTCCCCTG AGCTGGCTCT TACCATTGCC CTCAATCCTG GAGGGCGCCC CAAAGAGCCT 
CATTTGCATA GCTACAAGGA GGCCTTTGAG GAGATGGAGG GGACCTCCCC AAGCAGCCCG 
CCACACAGTG TGGCGCGCTC TCCTCCAGGT CTGGCCAAGA CACCCCTGTC TGCTCTGGGC 
CTGAAACCCC ACAACCCAGC AGACATCTTG CTGCACCCCA CAGGTGTTGC CAGAAGACTG 
ATCCAGCCAG AGGAAGACGA GGGGGAGGAG GTGACTAAGC CTCCAGAAGA GCCCCGGAGC 
TATGTGGAGT CCGTGGCGAG GACAGCGGTG GCAGGGCCTC GAGCTCAGGA CGTTGAGCCT 
AAGAGCTTCA GCGCACCAGC TGCTCACGCC TATGGGCACG AGACACCCCT GAGGAACGGG 
ACCCCAGGGG GCTCCTTTGT CTCCCCGAGC CCCCTCTCCA CAAGCAGCCC CATCCTCAGT 
GCTGACAGCA CTTCCGTGGG CAGTTTCCCA TCAGTGGTGA GCAGTGACCA GGGTCCCCGG 
ACACCCTTCC AGCCTATGCT GGACTCCAGC ATCCGCTCAG GCAGCTTGGG GCAGCCGAGC 
CCTGCAGCAC TGAGTTATCA GAGCTCCTCG CCTGTCCCTG TTGGTGGCAG CAGCTACAAC 
AGCCCTGACT ACTCCCTCCA ACCATTCAGC TCTTCTCCAG AAAGCCAAGG CCAGCCACAG 
TACAGTGCGG CCAGTGTCCA CATGGTACCT GGGAGCCCTC AGGCACGACA CAGGACAGTG 
GGTACCAACA CTCCACCTAG CCCTGGTTTT GGCCGGCGGG CCGTCAACCC CACCATGGCT 
GCCCCTGGTA GTCCCAGTTT GAGCCATCGG CAGGTGATGG GTCCATCTGG TCCAGGTTTC 
CATGGTAACG TGGTTTCTGG CCACCCAGCC AGTGCAGCTA CCACTCCTGG AAGCCCCAGC 
CTGGGCCGGC ACCCAGTGGG AAGCCATCAA GTTCCAGGCC TCCACAGCAG TGTGGTCACC 
ACTCCGGGAA GCCCAAGCCT GGGCCGACAC CCTGGTGCCC ACCAAGGGAA TCTGGCCTCC 
AGTCTCCACA GCAATGCAGT CATCAGCCCT GGGAGCCCAA GCTTGGGCCG GCACCTGGGT 
GGGTCTGGAT CCGTGGTTCC TGGAAGCCCC AGCTTGGACC GGCATGCAGC TTATGGTGGC 
TACTCTACCC CTGAGGACCG AAGACCAACA CTGTCCCGGC AAAGCAGTGC CTCTGGCTAC 
CAGGCCCCAT CCACACCGTC ATTCCCTGTC TCTCCTGCCT ACTACCCTGG CCTGAGCAGC 
CCTGCCACCT CACCCTCGCC AGACTCAGCA GCCTTCCGGC AGGGGAGCCC CACACCAGCC 
TTACCAGAGA AGCGAAGAAT GTCAGTGGGA GACCGGGCAG GCAGCCTCCC CAACTATGCC 
ACCATCAATG GGAAAGTGTC TTCCTCGCCT GTCGCCAATG GCATGGCCAG TGGGAGCAGC 
ACCGTCTCCT TCTCTCACAC GCTTCCGGAC TTTTCTAAGT ATTCGATGCC AGACAATAGT 
CCGGAGACTC GGGCTAAAGT GAAGTTTGTC CAGGACACTT CCAAGTATTG GTACAAGCCT 
GAGATCTCCA GAGAACAGGC CATCGCACTC CTAAAGGACC AGGAGCCAGG AGCCTTCATC 
ATCCGGGACA GCCACTCCTT CCGAGGGGCT TATGGGCTGG CCATGAAGGT GTCCTCACCC 
CCTCCAACCA TCACTCAGCA GGGCAAGAAA GGAGACATGA CCCATGAGCT GGTGAGGCAC 
TTTCTGATCG AGACAGGCCC TAGAGGAGTG AAGCTCAAGG GTTGTCCCAA TGAGCCAAAC 
TTTGGTTCCC TTTCTGCCCT GGTCTATCAG CACTCCGTCA TCCCACTGGC CCTGCCCTGT 
AAACTGGTCA TTCCAAGCCG AGACCCCACA GATGAATCAA AAGATAGCTC AGGCCCTGCC 
AACTCAACCA CTGACCTGCT GAAGCAAGGA GCAGCTTGCA ATGTGCTCTT CGTCAACTCT 
GTGGATATGG AGTCACTCAC CGGGCCACAG GCCATTTCCA AAGCCACATC TGAGACTCTG 
GCTGCCGACC CCACACCGGC TGCCACCATT GTTCACTTCA AGGTCTCTGC CCAAGGAATC 
ACACTGACTG ACAATCAAAG AAAGCTCTTC TTTAGAAGAC ACTACCCCCT CAACACTGTC 
ACCTTCTGTG ACCTGGATCC ACAAGAAAGA AAGTGGATGA AGACAGAGGG AGGCGCCCCT 
GCTAAGCTCT TTGGCTTCGT GGCCCGGAAG CAGGGCAGCA CCACAGACAA CGCTTGCCAC 
CTCTTCGCAG AGCTTGACCC CAACCAGCCT GCGTCTGCCA TCGTCAACTT CGTCTCCAAG 
GTCATGCTGA GTGCTGGCCA GAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495946.1
CDS	445..4314
Translation	MYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495883.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495883.1

ORF Insert Sequence:

ATGTACCGGG CTCAGCAACT CAGATCCCAT CCAGGCGGGG GCCCGACTGT GCCCTCCCCT 
GGCCGCCACA TTGTCCCAGC CCAGGTTCAC GTCAATGGTG GGGCCCTGGC ATCTGAGCGG 
GAGACAGACA TTCTAGATGA TGAGCTGCCC ATCCAGGACG GGCAGAGTGG GGGCAGCATG 
GGAACGCTGT CCTCCCTGGA TGGGGTCACC AACACCAGTG AAAGTGGCTA CCCAGAGACC 
CTGTCCCCAC TGACCAATGG CCTGGACAAA CCCTATTCCA CAGAACCTGT GCTCAACGGC 
GGAGGCTACC CCTATGAGGC TGCCAACCGG GTGATTCCTG TCCACAGCAG TCACTCGGCC 
CCCATTCGAC CCTCCTACTC TGCTCAGGAG GGTTTAGCTG GCTACCAGCG TGAGGGACCC 
CACCCAGCCT GGTCACAGCA AGTGACCAGT GCCCACTGTG GCTGTGACCC CAGTGGTCTA 
TTCAGATCCC AGTCCTTTCC AGATGTGGAG CCCCAGCTGC CCCAAGCTCC AACCCGTGGG 
GGTAGTAGCC GAGAGGCTGT GCAGAGGGGT CTGAATTCAT GGCAACAGCA GCAGCCCCAC 
CCACCTCCCC GCCAGCAGGA GAGATCCCCA CTGCAGAGTC TTGCCCGCAG CAAGCCTAGC 
CCCCAGCTGT CAGCAGAGAC CCCCGTAGCT GCTCTCCCAG AGTTCCCAAG GGCAGCGTCC 
CAGCAGGAGA TCGAGCAGTC CATCGAAACC CTCAATATGT TGATGCTGGA CCTGGAGCCA 
GCCTCCGCAG CTGCCCCTCT TCATAAGTCC CAGAGTGTCC CTGGGGCCTG GCCAGGGGCT 
TCTCCACTCT CTTCCCAGCC CCTCTTGGGG TCTTCCCGCC AGTCCCACCC ACTTACCCAG 
TCCCGATCTG GCTATATCCC CAGTGGGCAT TCTCTGGGAA CCCCGGAGCT GGTCTCTTCT 
GGCAGGCCTT ATTCACCTTA CGATTATCAG CTGCATCCAG CTGGATCCAA CCAGAGTTTC 
CATCCAAAAA GTCCAGCCTC TTCCACCTTC CTTCCAAGCC CTCATAGCTC TGCGGGGCCT 
CAGGAGCCCC CAGCCTCTCT CCCTGGTCTC ATCGCTCAGC CCCAGCTCCC ACCAAAGGAA 
ACTACATCAG ATCCCTCCCG AACTCCAGAG GAAGAGCCGC TGAACCTGGA AGGGCTGGTA 
GCCCATCGGG TAGCAGTGTA CAATGCCAGA CTACAGAGCC TGAGGCAGAG CCGCGGCTTC 
CAACCCTCTC CCCACTGGCG CCGATCTGCC TCCCTCTCTG GGGTTCAGGC TCGGGAGAGG 
CAGCCTGCAG AGCCCCCAGG CCCACTCCGG AGGCGGGCAG CCAGTGATGG ACAGTATGAA 
AACCAGTCTC CGGAGGCCAC ATCCCCCCGT AGTCCTGGGG TCCGCTCCCC CGTCCAGTGC 
GTCTCCCCTG AGCTGGCTCT TACCATTGCC CTCAATCCTG GAGGGCGCCC CAAAGAGCCT 
CATTTGCATA GCTACAAGGA GGCCTTTGAG GAGATGGAGG GGACCTCCCC AAGCAGCCCG 
CCACACAGTG TGGCGCGCTC TCCTCCAGGT CTGGCCAAGA CACCCCTGTC TGCTCTGGGC 
CTGAAACCCC ACAACCCAGC AGACATCTTG CTGCACCCCA CAGGTGTTGC CAGAAGACTG 
ATCCAGCCAG AGGAAGACGA GGGGGAGGAG GTGACTAAGC CTCCAGAAGA GCCCCGGAGC 
TATGTGGAGT CCGTGGCGAG GACAGCGGTG GCAGGGCCTC GAGCTCAGGA CGTTGAGCCT 
AAGAGCTTCA GCGCACCAGC TGCTCACGCC TATGGGCACG AGACACCCCT GAGGAACGGG 
ACCCCAGGGG GCTCCTTTGT CTCCCCGAGC CCCCTCTCCA CAAGCAGCCC CATCCTCAGT 
GCTGACAGCA CTTCCGTGGG CAGTTTCCCA TCAGTGGTGA GCAGTGACCA GGGTCCCCGG 
ACACCCTTCC AGCCTATGCT GGACTCCAGC ATCCGCTCAG GCAGCTTGGG GCAGCCGAGC 
CCTGCAGCAC TGAGTTATCA GAGCTCCTCG CCTGTCCCTG TTGGTGGCAG CAGCTACAAC 
AGCCCTGACT ACTCCCTCCA ACCATTCAGC TCTTCTCCAG AAAGCCAAGG CCAGCCACAG 
TACAGTGCGG CCAGTGTCCA CATGGTACCT GGGAGCCCTC AGGCACGACA CAGGACAGTG 
GGTACCAACA CTCCACCTAG CCCTGGTTTT GGCCGGCGGG CCGTCAACCC CACCATGGCT 
GCCCCTGGTA GTCCCAGTTT GAGCCATCGG CAGGTGATGG GTCCATCTGG TCCAGGTTTC 
CATGGTAACG TGGTTTCTGG CCACCCAGCC AGTGCAGCTA CCACTCCTGG AAGCCCCAGC 
CTGGGCCGGC ACCCAGTGGG AAGCCATCAA GTTCCAGGCC TCCACAGCAG TGTGGTCACC 
ACTCCGGGAA GCCCAAGCCT GGGCCGACAC CCTGGTGCCC ACCAAGGGAA TCTGGCCTCC 
AGTCTCCACA GCAATGCAGT CATCAGCCCT GGGAGCCCAA GCTTGGGCCG GCACCTGGGT 
GGGTCTGGAT CCGTGGTTCC TGGAAGCCCC AGCTTGGACC GGCATGCAGC TTATGGTGGC 
TACTCTACCC CTGAGGACCG AAGACCAACA CTGTCCCGGC AAAGCAGTGC CTCTGGCTAC 
CAGGCCCCAT CCACACCGTC ATTCCCTGTC TCTCCTGCCT ACTACCCTGG CCTGAGCAGC 
CCTGCCACCT CACCCTCGCC AGACTCAGCA GCCTTCCGGC AGGGGAGCCC CACACCAGCC 
TTACCAGAGA AGCGAAGAAT GTCAGTGGGA GACCGGGCAG GCAGCCTCCC CAACTATGCC 
ACCATCAATG GGAAAGTGTC TTCCTCGCCT GTCGCCAATG GCATGGCCAG TGGGAGCAGC 
ACCGTCTCCT TCTCTCACAC GCTTCCGGAC TTTTCTAAGT ATTCGATGCC AGACAATAGT 
CCGGAGACTC GGGCTAAAGT GAAGTTTGTC CAGGACACTT CCAAGTATTG GTACAAGCCT 
GAGATCTCCA GAGAACAGGC CATCGCACTC CTAAAGGACC AGGAGCCAGG AGCCTTCATC 
ATCCGGGACA GCCACTCCTT CCGAGGGGCT TATGGGCTGG CCATGAAGGT GTCCTCACCC 
CCTCCAACCA TCACTCAGCA GGGCAAGAAA GGAGACATGA CCCATGAGCT GGTGAGGCAC 
TTTCTGATCG AGACAGGCCC TAGAGGAGTG AAGCTCAAGG GTTGTCCCAA TGAGCCAAAC 
TTTGGTTCCC TTTCTGCCCT GGTCTATCAG CACTCCGTCA TCCCACTGGC CCTGCCCTGT 
AAACTGGTCA TTCCAAGCCG AGACCCCACA GATGAATCAA AAGATAGCTC AGGCCCTGCC 
AACTCAACCA CTGACCTGCT GAAGCAAGGA GCAGCTTGCA ATGTGCTCTT CGTCAACTCT 
GTGGATATGG AGTCACTCAC CGGGCCACAG GCCATTTCCA AAGCCACATC TGAGACTCTG 
GCTGCCGACC CCACACCGGC TGCCACCATT GTTCACTTCA AGGTCTCTGC CCAAGGAATC 
ACACTGACTG ACAATCAAAG AAAGCTCTTC TTTAGAAGAC ACTACCCCCT CAACACTGTC 
ACCTTCTGTG ACCTGGATCC ACAAGAAAGA AAGTGGATGA AGACAGAGGG AGGCGCCCCT 
GCTAAGCTCT TTGGCTTCGT GGCCCGGAAG CAGGGCAGCA CCACAGACAA CGCTTGCCAC 
CTCTTCGCAG AGCTTGACCC CAACCAGCCT GCGTCTGCCA TCGTCAACTT CGTCTCCAAG 
GTCATGCTGA GTGCTGGCCA GAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495871.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495934.1 Mus musculus tensin 1 (Tns1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu27359
Clone ID Related Accession (Same CDS sequence)	XM_006495871.3 , XM_006495871.4
Accession Version	XM_006495871.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495871.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCA CCGTGAGCCT GGTGTGCTGC GAGGCGCTCG AGCCTCTGCC CTCCTGCGGC 
CCGCAGCCCC CCGGGACTCC GCCGGGCCCC GCGCGGCCCG AGCGCTGTGA GCCCGGGGGT 
GCGGCGCCCG ACCCGAGGAG GCGGCTGCTG CTTCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGGTT TAAAAGTGGT GGGACACACC CAAGGGCCGC TGGATGGGAG CCTGTACGCC 
AAGGTGAAGA AGAAGGATTC CCTGAATGGC AGTTCGGGGC CTGTCACTAC TGCACGGCCT 
GCCCTGTCAG CCACTCCCAA CCACGTGGAA CACACCCTGT CTGTGAGCAG CGACTCTGGC 
AACTCCACAG CCTCCACTAA GACAGACAAG ACCGATGAGC CAGTCTCTGG GGCTACCACT 
GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG AAAGAGCTGG ACCGCCTACT AAGTGGCTTT 
GGTGTGGATA GAGAAAAGCA AGGCGCCATG TACCGGGCTC AGCAACTCAG ATCCCATCCA 
GGCGGGGGCC CGACTGTGCC CTCCCCTGGC CGCCACATTG TCCCAGCCCA GGTTCACGTC 
AATGGTGGGG CCCTGGCATC TGAGCGGGAG ACAGACATTC TAGATGATGA GCTGCCCATC 
CAGGACGGGC AGAGTGGGGG CAGCATGGGA ACGCTGTCCT CCCTGGATGG GGTCACCAAC 
ACCAGTGAAA GTGGCTACCC AGAGACCCTG TCCCCACTGA CCAATGGCCT GGACAAACCC 
TATTCCACAG AACCTGTGCT CAACGGCGGA GGCTACCCCT ATGAGGCTGC CAACCGGGTG 
ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC ATTCGACCCT CCTACTCTGC TCAGGAGGGT 
TTAGCTGGCT ACCAGCGTGA GGGACCCCAC CCAGCCTGGT CACAGCAAGT GACCAGTGCC 
CACTGTGGCT GTGACCCCAG TGGTCTATTC AGATCCCAGT CCTTTCCAGA TGTGGAGCCC 
CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG 
AATTCATGGC AACAGCAGCA GCCCCACCCA CCTCCCCGCC AGCAGGAGAG ATCCCCACTG 
CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC CAGCTGTCAG CAGAGACCCC CGTAGCTGCT 
CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG CAGGAGATCG AGCAGTCCAT CGAAACCCTC 
AATATGTTGA TGCTGGACCT GGAGCCAGCC TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG 
AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT CCACTCTCTT CCCAGCCCCT CTTGGGGTCT 
TCCCGCCAGT CCCACCCACT TACCCAGTCC CGATCTGGCT ATATCCCCAG TGGGCATTCT 
CTGGGAACCC CGGAGCTGGT CTCTTCTGGC AGGCCTTATT CACCTTACGA TTATCAGCTG 
CATCCAGCTG GATCCAACCA GAGTTTCCAT CCAAAAAGTC CAGCCTCTTC CACCTTCCTT 
CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG GAGCCCCCAG CCTCTCTCCC TGGTCTCATC 
GCTCAGCCCC AGCTCCCACC AAAGGAAACT ACATCAGATC CCTCCCGAAC TCCAGAGGAA 
GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC CATCGGGTAG CAGTGTACAA TGCCAGACTA 
CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC 
CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG 
CGGGCAGCCA GTGATGGACA GTATGAAAAC CAGTCTCCGG AGGCCACATC CCCCCGTAGT 
CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC TCCCCTGAGC TGGCTCTTAC CATTGCCCTC 
AATCCTGGAG GGCGCCCCAA AGAGCCTCAT TTGCATAGCT ACAAGGAGGC CTTTGAGGAG 
ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA CACAGTGTGG CGCGCTCTCC TCCAGGTCTG 
GCCAAGACAC CCCTGTCTGC TCTGGGCCTG AAACCCCACA ACCCAGCAGA CATCTTGCTG 
CACCCCACAG GTGTTGCCAG AAGACTGATC CAGCCAGAGG AAGACGAGGG GGAGGAGGTG 
ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA 
GGGCCTCGAG CTCAGGACGT TGAGCCTAAG AGCTTCAGCG CACCAGCTGC TCACGCCTAT 
GGGCACGAGA CACCCCTGAG GAACGGGACC CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC 
CTCTCCACAA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CCGTGGGCAG TTTCCCATCA 
GTGGTGAGCA GTGACCAGGG TCCCCGGACA CCCTTCCAGC CTATGCTGGA CTCCAGCATC 
CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT GCAGCACTGA GTTATCAGAG CTCCTCGCCT 
GTCCCTGTTG GTGGCAGCAG CTACAACAGC CCTGACTACT CCCTCCAACC ATTCAGCTCT 
TCTCCAGAAA GCCAAGGCCA GCCACAGTAC AGTGCGGCCA GTGTCCACAT GGTACCTGGG 
AGCCCTCAGG CACGACACAG GACAGTGGGT ACCAACACTC CACCTAGCCC TGGTTTTGGC 
CGGCGGGCCG TCAACCCCAC CATGGCTGCC CCTGGTAGTC CCAGTTTGAG CCATCGGCAG 
GTGATGGGTC CATCTGGTCC AGGTTTCCAT GGTAACGTGG TTTCTGGCCA CCCAGCCAGT 
GCAGCTACCA CTCCTGGAAG CCCCAGCCTG GGCCGGCACC CAGTGGGAAG CCATCAAGTT 
CCAGGCCTCC ACAGCAGTGT GGTCACCACT CCGGGAAGCC CAAGCCTGGG CCGACACCCT 
GGTGCCCACC AAGGGAATCT GGCCTCCAGT CTCCACAGCA ATGCAGTCAT CAGCCCTGGG 
AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG TCTGGATCCG TGGTTCCTGG AAGCCCCAGC 
TTGGACCGGC ATGCAGCTTA TGGTGGCTAC TCTACCCCTG AGGACCGAAG ACCAACACTG 
TCCCGGCAAA GCAGTGCCTC TGGCTACCAG GCCCCATCCA CACCGTCATT CCCTGTCTCT 
CCTGCCTACT ACCCTGGCCT GAGCAGCCCT GCCACCTCAC CCTCGCCAGA CTCAGCAGCC 
TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA CCAGAGAAGC GAAGAATGTC AGTGGGAGAC 
CGGGCAGGCA GCCTCCCCAA CTATGCCACC ATCAATGGGA AAGTGTCTTC CTCGCCTGTC 
GCCAATGGCA TGGCCAGTGG GAGCAGCACC GTCTCCTTCT CTCACACGCT TCCGGACTTT 
TCTAAGTATT CGATGCCAGA CAATAGTCCG GAGACTCGGG CTAAAGTGAA GTTTGTCCAG 
GACACTTCCA AGTATTGGTA CAAGCCTGAG ATCTCCAGAG AACAGGCCAT CGCACTCCTA 
AAGGACCAGG AGCCAGGAGC CTTCATCATC CGGGACAGCC ACTCCTTCCG AGGGGCTTAT 
GGGCTGGCCA TGAAGGTGTC CTCACCCCCT CCAACCATCA CTCAGCAGGG CAAGAAAGGA 
GACATGACCC ATGAGCTGGT GAGGCACTTT CTGATCGAGA CAGGCCCTAG AGGAGTGAAG 
CTCAAGGGTT GTCCCAATGA GCCAAACTTT GGTTCCCTTT CTGCCCTGGT CTATCAGCAC 
TCCGTCATCC CACTGGCCCT GCCCTGTAAA CTGGTCATTC CAAGCCGAGA CCCCACAGAT 
GAATCAAAAG ATAGCTCAGG CCCTGCCAAC TCAACCACTG ACCTGCTGAA GCAAGGAGCA 
GCTTGCAATG TGCTCTTCGT CAACTCTGTG GATATGGAGT CACTCACCGG GCCACAGGCC 
ATTTCCAAAG CCACATCTGA GACTCTGGCT GCCGACCCCA CACCGGCTGC CACCATTGTT 
CACTTCAAGG TCTCTGCCCA AGGAATCACA CTGACTGACA ATCAAAGAAA GCTCTTCTTT 
AGAAGACACT ACCCCCTCAA CACTGTCACC TTCTGTGACC TGGATCCACA AGAAAGAAAG 
TGGATGAAGA CAGAGGGAGG CGCCCCTGCT AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG 
GGCAGCACCA CAGACAACGC TTGCCACCTC TTCGCAGAGC TTGACCCCAA CCAGCCTGCG 
TCTGCCATCG TCAACTTCGT CTCCAAGGTC ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495934.1
CDS	84..5840
Translation	MGCTVSLVCCEALEPLPSCGPQPPGTPPGPARPERCEPGGAAPDPRRRLLLQPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495871.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495871.4

ORF Insert Sequence:

ATGGGCTGCA CCGTGAGCCT GGTGTGCTGC GAGGCGCTCG AGCCTCTGCC CTCCTGCGGC 
CCGCAGCCCC CCGGGACTCC GCCGGGCCCC GCGCGGCCCG AGCGCTGTGA GCCCGGGGGT 
GCGGCGCCCG ACCCGAGGAG GCGGCTGCTG CTTCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGGTT TAAAAGTGGT GGGACACACC CAAGGGCCGC TGGATGGGAG CCTGTACGCC 
AAGGTGAAGA AGAAGGATTC CCTGAATGGC AGTTCGGGGC CTGTCACTAC TGCACGGCCT 
GCCCTGTCAG CCACTCCCAA CCACGTGGAA CACACCCTGT CTGTGAGCAG CGACTCTGGC 
AACTCCACAG CCTCCACTAA GACAGACAAG ACCGATGAGC CAGTCTCTGG GGCTACCACT 
GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG AAAGAGCTGG ACCGCCTACT AAGTGGCTTT 
GGTGTGGATA GAGAAAAGCA AGGCGCCATG TACCGGGCTC AGCAACTCAG ATCCCATCCA 
GGCGGGGGCC CGACTGTGCC CTCCCCTGGC CGCCACATTG TCCCAGCCCA GGTTCACGTC 
AATGGTGGGG CCCTGGCATC TGAGCGGGAG ACAGACATTC TAGATGATGA GCTGCCCATC 
CAGGACGGGC AGAGTGGGGG CAGCATGGGA ACGCTGTCCT CCCTGGATGG GGTCACCAAC 
ACCAGTGAAA GTGGCTACCC AGAGACCCTG TCCCCACTGA CCAATGGCCT GGACAAACCC 
TATTCCACAG AACCTGTGCT CAACGGCGGA GGCTACCCCT ATGAGGCTGC CAACCGGGTG 
ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC ATTCGACCCT CCTACTCTGC TCAGGAGGGT 
TTAGCTGGCT ACCAGCGTGA GGGACCCCAC CCAGCCTGGT CACAGCAAGT GACCAGTGCC 
CACTGTGGCT GTGACCCCAG TGGTCTATTC AGATCCCAGT CCTTTCCAGA TGTGGAGCCC 
CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG 
AATTCATGGC AACAGCAGCA GCCCCACCCA CCTCCCCGCC AGCAGGAGAG ATCCCCACTG 
CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC CAGCTGTCAG CAGAGACCCC CGTAGCTGCT 
CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG CAGGAGATCG AGCAGTCCAT CGAAACCCTC 
AATATGTTGA TGCTGGACCT GGAGCCAGCC TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG 
AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT CCACTCTCTT CCCAGCCCCT CTTGGGGTCT 
TCCCGCCAGT CCCACCCACT TACCCAGTCC CGATCTGGCT ATATCCCCAG TGGGCATTCT 
CTGGGAACCC CGGAGCTGGT CTCTTCTGGC AGGCCTTATT CACCTTACGA TTATCAGCTG 
CATCCAGCTG GATCCAACCA GAGTTTCCAT CCAAAAAGTC CAGCCTCTTC CACCTTCCTT 
CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG GAGCCCCCAG CCTCTCTCCC TGGTCTCATC 
GCTCAGCCCC AGCTCCCACC AAAGGAAACT ACATCAGATC CCTCCCGAAC TCCAGAGGAA 
GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC CATCGGGTAG CAGTGTACAA TGCCAGACTA 
CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC 
CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG 
CGGGCAGCCA GTGATGGACA GTATGAAAAC CAGTCTCCGG AGGCCACATC CCCCCGTAGT 
CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC TCCCCTGAGC TGGCTCTTAC CATTGCCCTC 
AATCCTGGAG GGCGCCCCAA AGAGCCTCAT TTGCATAGCT ACAAGGAGGC CTTTGAGGAG 
ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA CACAGTGTGG CGCGCTCTCC TCCAGGTCTG 
GCCAAGACAC CCCTGTCTGC TCTGGGCCTG AAACCCCACA ACCCAGCAGA CATCTTGCTG 
CACCCCACAG GTGTTGCCAG AAGACTGATC CAGCCAGAGG AAGACGAGGG GGAGGAGGTG 
ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA 
GGGCCTCGAG CTCAGGACGT TGAGCCTAAG AGCTTCAGCG CACCAGCTGC TCACGCCTAT 
GGGCACGAGA CACCCCTGAG GAACGGGACC CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC 
CTCTCCACAA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CCGTGGGCAG TTTCCCATCA 
GTGGTGAGCA GTGACCAGGG TCCCCGGACA CCCTTCCAGC CTATGCTGGA CTCCAGCATC 
CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT GCAGCACTGA GTTATCAGAG CTCCTCGCCT 
GTCCCTGTTG GTGGCAGCAG CTACAACAGC CCTGACTACT CCCTCCAACC ATTCAGCTCT 
TCTCCAGAAA GCCAAGGCCA GCCACAGTAC AGTGCGGCCA GTGTCCACAT GGTACCTGGG 
AGCCCTCAGG CACGACACAG GACAGTGGGT ACCAACACTC CACCTAGCCC TGGTTTTGGC 
CGGCGGGCCG TCAACCCCAC CATGGCTGCC CCTGGTAGTC CCAGTTTGAG CCATCGGCAG 
GTGATGGGTC CATCTGGTCC AGGTTTCCAT GGTAACGTGG TTTCTGGCCA CCCAGCCAGT 
GCAGCTACCA CTCCTGGAAG CCCCAGCCTG GGCCGGCACC CAGTGGGAAG CCATCAAGTT 
CCAGGCCTCC ACAGCAGTGT GGTCACCACT CCGGGAAGCC CAAGCCTGGG CCGACACCCT 
GGTGCCCACC AAGGGAATCT GGCCTCCAGT CTCCACAGCA ATGCAGTCAT CAGCCCTGGG 
AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG TCTGGATCCG TGGTTCCTGG AAGCCCCAGC 
TTGGACCGGC ATGCAGCTTA TGGTGGCTAC TCTACCCCTG AGGACCGAAG ACCAACACTG 
TCCCGGCAAA GCAGTGCCTC TGGCTACCAG GCCCCATCCA CACCGTCATT CCCTGTCTCT 
CCTGCCTACT ACCCTGGCCT GAGCAGCCCT GCCACCTCAC CCTCGCCAGA CTCAGCAGCC 
TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA CCAGAGAAGC GAAGAATGTC AGTGGGAGAC 
CGGGCAGGCA GCCTCCCCAA CTATGCCACC ATCAATGGGA AAGTGTCTTC CTCGCCTGTC 
GCCAATGGCA TGGCCAGTGG GAGCAGCACC GTCTCCTTCT CTCACACGCT TCCGGACTTT 
TCTAAGTATT CGATGCCAGA CAATAGTCCG GAGACTCGGG CTAAAGTGAA GTTTGTCCAG 
GACACTTCCA AGTATTGGTA CAAGCCTGAG ATCTCCAGAG AACAGGCCAT CGCACTCCTA 
AAGGACCAGG AGCCAGGAGC CTTCATCATC CGGGACAGCC ACTCCTTCCG AGGGGCTTAT 
GGGCTGGCCA TGAAGGTGTC CTCACCCCCT CCAACCATCA CTCAGCAGGG CAAGAAAGGA 
GACATGACCC ATGAGCTGGT GAGGCACTTT CTGATCGAGA CAGGCCCTAG AGGAGTGAAG 
CTCAAGGGTT GTCCCAATGA GCCAAACTTT GGTTCCCTTT CTGCCCTGGT CTATCAGCAC 
TCCGTCATCC CACTGGCCCT GCCCTGTAAA CTGGTCATTC CAAGCCGAGA CCCCACAGAT 
GAATCAAAAG ATAGCTCAGG CCCTGCCAAC TCAACCACTG ACCTGCTGAA GCAAGGAGCA 
GCTTGCAATG TGCTCTTCGT CAACTCTGTG GATATGGAGT CACTCACCGG GCCACAGGCC 
ATTTCCAAAG CCACATCTGA GACTCTGGCT GCCGACCCCA CACCGGCTGC CACCATTGTT 
CACTTCAAGG TCTCTGCCCA AGGAATCACA CTGACTGACA ATCAAAGAAA GCTCTTCTTT 
AGAAGACACT ACCCCCTCAA CACTGTCACC TTCTGTGACC TGGATCCACA AGAAAGAAAG 
TGGATGAAGA CAGAGGGAGG CGCCCCTGCT AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG 
GGCAGCACCA CAGACAACGC TTGCCACCTC TTCGCAGAGC TTGACCCCAA CCAGCCTGCG 
TCTGCCATCG TCAACTTCGT CTCCAAGGTC ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495874.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495937.1 Mus musculus tensin 1 (Tns1), transcript variant X17, mRNA.

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CloneID	OMu27362
Clone ID Related Accession (Same CDS sequence)	XM_006495874.4 , XM_006495874.3
Accession Version	XM_006495874.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495874.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGT GCAAGGGTGC AGGGATACAG CCCAGCGGTC TGCATGCACT GATGTCTGGC 
CAGCCTCAGA TCCTGAACTT GGGGAAGGTG GCTGCCCCTT GCGTTCCTCC GTCCAGCCAT 
GAACTGGTGC CCATCACCAC AGAGACCGTA CCCAAGAATG TAGTAGATGT GGGGGAAGGA 
GACTGCCGGG TTGGAAGTTC TCCGAAAAAC CTGGAGGAGG GCGGCTCCAT GAGAGTTTCT 
CCAAGCATAC AGCCACAACC ACAATCACAG CCTACCAGTC TCTCTAGAAA CACGAGTGTG 
AGCCGAGCCA TGGAAGACAG TTGTGAGCTG GACCTGGTGT ATGTCACCGA ACGCATCATC 
GCCGTGTCCT TCCCTAGCAC AGCCAATGAG GAGAACTTCC GCAGCAACCT CCGTGAAGTG 
GCTCAGATGT TGAAGTCCAA GCATGGGGGC AACTACCTGC TATTCAACCT CTCTGAACAG 
AGGCCTGACA TCACCAAGCT GCATGCTAAG GTCCTGGAAT TCGGCTGGCC TGACCTGCAC 
ACACCGGCCC TGGAGAAGAT ATGCAGTGTC TGTAAGGCCA TGGACACCTG GCTCAATGCA 
GACCCCCACA ATGTCGTTGT TCTCCACAAC AAGGGAAACC GAGGCAGGAT TGGGGTTGTC 
ATTGCAGCGT ATCTGCACTA CAGCAACATT TCAGCCAGTG CTGACCAGGC TCTGGACCGG 
TTTGCAATGA AGAGATTCTA TGAGGACAAG ATCGTGCCCA TTGGCCAGCC ATCCCAGAGG 
AGATATGTGC ACTACTTCAG TGGCCTACTC TCTGGCTCCA TCAAAATGAA CAACAAGCCT 
TTGTTTCTGC ATCATGTGAT CATGCACGGC ATCCCCAACT TTGAGTCTAA AGGAGGGTGC 
CGGCCCTTTC TCCGCATCTA CCAAGCCATG CAACCTGTGT ACACATCTGG CATTTACAAC 
ATCCCTGGAG ACAGCCAGGC CAGCATCTGT ATCACCATTG AACCAGGGCT GCTCTTGAAG 
GGAGACATCT TGCTGAAGTG TTATCATAAG AAATTCCGGA GCCCGGCTCG AGACGTCATC 
TTCCGAGTGC AGTTCCACAC CTGTGCCATC CATGACTTGG GGGTTGTTTT TGGGAAGGAA 
GATCTGGATG AGGCCTTCAA AGATGATCGA TTTCCTGACT ATGGCAAAGT GGAGTTTGTC 
TTTTCCTATG GACCAGAGAA AATTCAAGGC ATGGAACACC TGGAGAATGG GCCCAGCGTA 
TCTGTAGACT ATAACACTTC TGACCCCCTC ATCCGCTGGG ACTCCTATGA CAACTTCAGT 
GGGCATCGTG AGGATGGCAT GGAAGGTTTA AAAGTGGTGG GACACACCCA AGGGCCGCTG 
GATGGGAGCC TGTACGCCAA GGTGAAGAAG AAGGATTCCC TGAATGGCAG TTCGGGGCCT 
GTCACTACTG CACGGCCTGC CCTGTCAGCC ACTCCCAACC ACGTGGAACA CACCCTGTCT 
GTGAGCAGCG ACTCTGGCAA CTCCACAGCC TCCACTAAGA CAGACAAGAC CGATGAGCCA 
GTCTCTGGGG CTACCACTGC CCCTGCTGCC CTGAGTCCTC AGGAGAAGAA AGAGCTGGAC 
CGCCTACTAA GTGGCTTTGG TGTGGATAGA GAAAAGCAAG GCGCCATGTA CCGGGCTCAG 
CAACTCAGAT CCCATCCAGG CGGGGGCCCG ACTGTGCCCT CCCCTGGCCG CCACATTGTC 
CCAGCCCAGG TTCACGTCAA TGGTGGGGCC CTGGCATCTG AGCGGGAGAC AGACATTCTA 
GATGATGAGC TGCCCATCCA GGACGGGCAG AGTGGGGGCA GCATGGGAAC GCTGTCCTCC 
CTGGATGGGG TCACCAACAC CAGTGAAAGT GGCTACCCAG AGACCCTGTC CCCACTGACC 
AATGGCCTGG ACAAACCCTA TTCCACAGAA CCTGTGCTCA ACGGCGGAGG CTACCCCTAT 
GAGGCTGCCA ACCGGGTGAT TCCTGTCCAC AGCAGTCACT CGGCCCCCAT TCGACCCTCC 
TACTCTGCTC AGGAGGGTTT AGCTGGCTAC CAGCGTGAGG GACCCCACCC AGCCTGGTCA 
CAGCAAGTGA CCAGTGCCCA CTGTGGCTGT GACCCCAGTG GTCTATTCAG ATCCCAGTCC 
TTTCCAGATG TGGAGCCCCA GCTGCCCCAA GCTCCAACCC GTGGGGGTAG TAGCCGAGAG 
GCTGTGCAGA GGGGTCTGAA TTCATGGCAA CAGCAGCAGC CCCACCCACC TCCCCGCCAG 
CAGGAGAGAT CCCCACTGCA GAGTCTTGCC CGCAGCAAGC CTAGCCCCCA GCTGTCAGCA 
GAGACCCCCG TAGCTGCTCT CCCAGAGTTC CCAAGGGCAG CGTCCCAGCA GGAGATCGAG 
CAGTCCATCG AAACCCTCAA TATGTTGATG CTGGACCTGG AGCCAGCCTC CGCAGCTGCC 
CCTCTTCATA AGTCCCAGAG TGTCCCTGGG GCCTGGCCAG GGGCTTCTCC ACTCTCTTCC 
CAGCCCCTCT TGGGGTCTTC CCGCCAGTCC CACCCACTTA CCCAGTCCCG ATCTGGCTAT 
ATCCCCAGTG GGCATTCTCT GGGAACCCCG GAGCTGGTCT CTTCTGGCAG GCCTTATTCA 
CCTTACGATT ATCAGCTGCA TCCAGCTGGA TCCAACCAGA GTTTCCATCC AAAAAGTCCA 
GCCTCTTCCA CCTTCCTTCC AAGCCCTCAT AGCTCTGCGG GGCCTCAGGA GCCCCCAGCC 
TCTCTCCCTG GTCTCATCGC TCAGCCCCAG CTCCCACCAA AGGAAACTAC ATCAGATCCC 
TCCCGAACTC CAGAGGAAGA GCCGCTGAAC CTGGAAGGGC TGGTAGCCCA TCGGGTAGCA 
GTGTACAATG CCAGACTACA GAGCCTGAGG CAGAGCCGCG GCTTCCAACC CTCTCCCCAC 
TGGCGCCGAT CTGCCTCCCT CTCTGGGGTT CAGGCTCGGG AGAGGCAGCC TGCAGAGCCC 
CCAGGCCCAC TCCGGAGGCG GGCAGCCAGT GATGGACAGT ATGAAAACCA GTCTCCGGAG 
GCCACATCCC CCCGTAGTCC TGGGGTCCGC TCCCCCGTCC AGTGCGTCTC CCCTGAGCTG 
GCTCTTACCA TTGCCCTCAA TCCTGGAGGG CGCCCCAAAG AGCCTCATTT GCATAGCTAC 
AAGGAGGCCT TTGAGGAGAT GGAGGGGACC TCCCCAAGCA GCCCGCCACA CAGTGTGGCG 
CGCTCTCCTC CAGGTCTGGC CAAGACACCC CTGTCTGCTC TGGGCCTGAA ACCCCACAAC 
CCAGCAGACA TCTTGCTGCA CCCCACAGGT GTTGCCAGAA GACTGATCCA GCCAGAGGAA 
GACGAGGGGG AGGAGGTGAC TAAGCCTCCA GAAGAGCCCC GGAGCTATGT GGAGTCCGTG 
GCGAGGACAG CGGTGGCAGG GCCTCGAGCT CAGGACGTTG AGCCTAAGAG CTTCAGCGCA 
CCAGCTGCTC ACGCCTATGG GCACGAGACA CCCCTGAGGA ACGGGACCCC AGGGGGCTCC 
TTTGTCTCCC CGAGCCCCCT CTCCACAAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCC 
GTGGGCAGTT TCCCATCAGT GGTGAGCAGT GACCAGGGTC CCCGGACACC CTTCCAGCCT 
ATGCTGGACT CCAGCATCCG CTCAGGCAGC TTGGGGCAGC CGAGCCCTGC AGCACTGAGT 
TATCAGAGCT CCTCGCCTGT CCCTGTTGGT GGCAGCAGCT ACAACAGCCC TGACTACTCC 
CTCCAACCAT TCAGCTCTTC TCCAGAAAGC CAAGGCCAGC CACAGTACAG TGCGGCCAGT 
GTCCACATGG TACCTGGGAG CCCTCAGGCA CGACACAGGA CAGTGGGTAC CAACACTCCA 
CCTAGCCCTG GTTTTGGCCG GCGGGCCGTC AACCCCACCA TGGCTGCCCC TGGTAGTCCC 
AGTTTGAGCC ATCGGCAGGT GATGGGTCCA TCTGGTCCAG GTTTCCATGG TAACGTGGTT 
TCTGGCCACC CAGCCAGTGC AGCTACCACT CCTGGAAGCC CCAGCCTGGG CCGGCACCCA 
GTGGGAAGCC ATCAAGTTCC AGGCCTCCAC AGCAGTGTGG TCACCACTCC GGGAAGCCCA 
AGCCTGGGCC GACACCCTGG TGCCCACCAA GGGAATCTGG CCTCCAGTCT CCACAGCAAT 
GCAGTCATCA GCCCTGGGAG CCCAAGCTTG GGCCGGCACC TGGGTGGGTC TGGATCCGTG 
GTTCCTGGAA GCCCCAGCTT GGACCGGCAT GCAGCTTATG GTGGCTACTC TACCCCTGAG 
GACCGAAGAC CAACACTGTC CCGGCAAAGC AGTGCCTCTG GCTACCAGGC CCCATCCACA 
CCGTCATTCC CTGTCTCTCC TGCCTACTAC CCTGGCCTGA GCAGCCCTGC CACCTCACCC 
TCGCCAGACT CAGCAGCCTT CCGGCAGGGG AGCCCCACAC CAGCCTTACC AGAGAAGCGA 
AGAATGTCAG TGGGAGACCG GGCAGGCAGC CTCCCCAACT ATGCCACCAT CAATGGGAAA 
GTGTCTTCCT CGCCTGTCGC CAATGGCATG GCCAGTGGGA GCAGCACCGT CTCCTTCTCT 
CACACGCTTC CGGACTTTTC TAAGTATTCG ATGCCAGACA ATAGTCCGGA GACTCGGGCT 
AAAGTGAAGT TTGTCCAGGA CACTTCCAAG TATTGGTACA AGCCTGAGAT CTCCAGAGAA 
CAGGCCATCG CACTCCTAAA GGACCAGGAG CCAGGAGCCT TCATCATCCG GGACAGCCAC 
TCCTTCCGAG GGGCTTATGG GCTGGCCATG AAGGTGTCCT CACCCCCTCC AACCATCACT 
CAGCAGGGCA AGAAAGGAGA CATGACCCAT GAGCTGGTGA GGCACTTTCT GATCGAGACA 
GGCCCTAGAG GAGTGAAGCT CAAGGGTTGT CCCAATGAGC CAAACTTTGG TTCCCTTTCT 
GCCCTGGTCT ATCAGCACTC CGTCATCCCA CTGGCCCTGC CCTGTAAACT GGTCATTCCA 
AGCCGAGACC CCACAGATGA ATCAAAAGAT AGCTCAGGCC CTGCCAACTC AACCACTGAC 
CTGCTGAAGC AAGGAGCAGC TTGCAATGTG CTCTTCGTCA ACTCTGTGGA TATGGAGTCA 
CTCACCGGGC CACAGGCCAT TTCCAAAGCC ACATCTGAGA CTCTGGCTGC CGACCCCACA 
CCGGCTGCCA CCATTGTTCA CTTCAAGGTC TCTGCCCAAG GAATCACACT GACTGACAAT 
CAAAGAAAGC TCTTCTTTAG AAGACACTAC CCCCTCAACA CTGTCACCTT CTGTGACCTG 
GATCCACAAG AAAGAAAGTG GATGAAGACA GAGGGAGGCG CCCCTGCTAA GCTCTTTGGC 
TTCGTGGCCC GGAAGCAGGG CAGCACCACA GACAACGCTT GCCACCTCTT CGCAGAGCTT 
GACCCCAACC AGCCTGCGTC TGCCATCGTC AACTTCGTCT CCAAGGTCAT GCTGAGTGCT 
GGCCAGAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495937.1
CDS	241..5775
Translation	MNVCKGAGIQPSGLHALMSGQPQILNLGKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495874.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495874.4

ORF Insert Sequence:

ATGAATGTGT GCAAGGGTGC AGGGATACAG CCCAGCGGTC TGCATGCACT GATGTCTGGC 
CAGCCTCAGA TCCTGAACTT GGGGAAGGTG GCTGCCCCTT GCGTTCCTCC GTCCAGCCAT 
GAACTGGTGC CCATCACCAC AGAGACCGTA CCCAAGAATG TAGTAGATGT GGGGGAAGGA 
GACTGCCGGG TTGGAAGTTC TCCGAAAAAC CTGGAGGAGG GCGGCTCCAT GAGAGTTTCT 
CCAAGCATAC AGCCACAACC ACAATCACAG CCTACCAGTC TCTCTAGAAA CACGAGTGTG 
AGCCGAGCCA TGGAAGACAG TTGTGAGCTG GACCTGGTGT ATGTCACCGA ACGCATCATC 
GCCGTGTCCT TCCCTAGCAC AGCCAATGAG GAGAACTTCC GCAGCAACCT CCGTGAAGTG 
GCTCAGATGT TGAAGTCCAA GCATGGGGGC AACTACCTGC TATTCAACCT CTCTGAACAG 
AGGCCTGACA TCACCAAGCT GCATGCTAAG GTCCTGGAAT TCGGCTGGCC TGACCTGCAC 
ACACCGGCCC TGGAGAAGAT ATGCAGTGTC TGTAAGGCCA TGGACACCTG GCTCAATGCA 
GACCCCCACA ATGTCGTTGT TCTCCACAAC AAGGGAAACC GAGGCAGGAT TGGGGTTGTC 
ATTGCAGCGT ATCTGCACTA CAGCAACATT TCAGCCAGTG CTGACCAGGC TCTGGACCGG 
TTTGCAATGA AGAGATTCTA TGAGGACAAG ATCGTGCCCA TTGGCCAGCC ATCCCAGAGG 
AGATATGTGC ACTACTTCAG TGGCCTACTC TCTGGCTCCA TCAAAATGAA CAACAAGCCT 
TTGTTTCTGC ATCATGTGAT CATGCACGGC ATCCCCAACT TTGAGTCTAA AGGAGGGTGC 
CGGCCCTTTC TCCGCATCTA CCAAGCCATG CAACCTGTGT ACACATCTGG CATTTACAAC 
ATCCCTGGAG ACAGCCAGGC CAGCATCTGT ATCACCATTG AACCAGGGCT GCTCTTGAAG 
GGAGACATCT TGCTGAAGTG TTATCATAAG AAATTCCGGA GCCCGGCTCG AGACGTCATC 
TTCCGAGTGC AGTTCCACAC CTGTGCCATC CATGACTTGG GGGTTGTTTT TGGGAAGGAA 
GATCTGGATG AGGCCTTCAA AGATGATCGA TTTCCTGACT ATGGCAAAGT GGAGTTTGTC 
TTTTCCTATG GACCAGAGAA AATTCAAGGC ATGGAACACC TGGAGAATGG GCCCAGCGTA 
TCTGTAGACT ATAACACTTC TGACCCCCTC ATCCGCTGGG ACTCCTATGA CAACTTCAGT 
GGGCATCGTG AGGATGGCAT GGAAGGTTTA AAAGTGGTGG GACACACCCA AGGGCCGCTG 
GATGGGAGCC TGTACGCCAA GGTGAAGAAG AAGGATTCCC TGAATGGCAG TTCGGGGCCT 
GTCACTACTG CACGGCCTGC CCTGTCAGCC ACTCCCAACC ACGTGGAACA CACCCTGTCT 
GTGAGCAGCG ACTCTGGCAA CTCCACAGCC TCCACTAAGA CAGACAAGAC CGATGAGCCA 
GTCTCTGGGG CTACCACTGC CCCTGCTGCC CTGAGTCCTC AGGAGAAGAA AGAGCTGGAC 
CGCCTACTAA GTGGCTTTGG TGTGGATAGA GAAAAGCAAG GCGCCATGTA CCGGGCTCAG 
CAACTCAGAT CCCATCCAGG CGGGGGCCCG ACTGTGCCCT CCCCTGGCCG CCACATTGTC 
CCAGCCCAGG TTCACGTCAA TGGTGGGGCC CTGGCATCTG AGCGGGAGAC AGACATTCTA 
GATGATGAGC TGCCCATCCA GGACGGGCAG AGTGGGGGCA GCATGGGAAC GCTGTCCTCC 
CTGGATGGGG TCACCAACAC CAGTGAAAGT GGCTACCCAG AGACCCTGTC CCCACTGACC 
AATGGCCTGG ACAAACCCTA TTCCACAGAA CCTGTGCTCA ACGGCGGAGG CTACCCCTAT 
GAGGCTGCCA ACCGGGTGAT TCCTGTCCAC AGCAGTCACT CGGCCCCCAT TCGACCCTCC 
TACTCTGCTC AGGAGGGTTT AGCTGGCTAC CAGCGTGAGG GACCCCACCC AGCCTGGTCA 
CAGCAAGTGA CCAGTGCCCA CTGTGGCTGT GACCCCAGTG GTCTATTCAG ATCCCAGTCC 
TTTCCAGATG TGGAGCCCCA GCTGCCCCAA GCTCCAACCC GTGGGGGTAG TAGCCGAGAG 
GCTGTGCAGA GGGGTCTGAA TTCATGGCAA CAGCAGCAGC CCCACCCACC TCCCCGCCAG 
CAGGAGAGAT CCCCACTGCA GAGTCTTGCC CGCAGCAAGC CTAGCCCCCA GCTGTCAGCA 
GAGACCCCCG TAGCTGCTCT CCCAGAGTTC CCAAGGGCAG CGTCCCAGCA GGAGATCGAG 
CAGTCCATCG AAACCCTCAA TATGTTGATG CTGGACCTGG AGCCAGCCTC CGCAGCTGCC 
CCTCTTCATA AGTCCCAGAG TGTCCCTGGG GCCTGGCCAG GGGCTTCTCC ACTCTCTTCC 
CAGCCCCTCT TGGGGTCTTC CCGCCAGTCC CACCCACTTA CCCAGTCCCG ATCTGGCTAT 
ATCCCCAGTG GGCATTCTCT GGGAACCCCG GAGCTGGTCT CTTCTGGCAG GCCTTATTCA 
CCTTACGATT ATCAGCTGCA TCCAGCTGGA TCCAACCAGA GTTTCCATCC AAAAAGTCCA 
GCCTCTTCCA CCTTCCTTCC AAGCCCTCAT AGCTCTGCGG GGCCTCAGGA GCCCCCAGCC 
TCTCTCCCTG GTCTCATCGC TCAGCCCCAG CTCCCACCAA AGGAAACTAC ATCAGATCCC 
TCCCGAACTC CAGAGGAAGA GCCGCTGAAC CTGGAAGGGC TGGTAGCCCA TCGGGTAGCA 
GTGTACAATG CCAGACTACA GAGCCTGAGG CAGAGCCGCG GCTTCCAACC CTCTCCCCAC 
TGGCGCCGAT CTGCCTCCCT CTCTGGGGTT CAGGCTCGGG AGAGGCAGCC TGCAGAGCCC 
CCAGGCCCAC TCCGGAGGCG GGCAGCCAGT GATGGACAGT ATGAAAACCA GTCTCCGGAG 
GCCACATCCC CCCGTAGTCC TGGGGTCCGC TCCCCCGTCC AGTGCGTCTC CCCTGAGCTG 
GCTCTTACCA TTGCCCTCAA TCCTGGAGGG CGCCCCAAAG AGCCTCATTT GCATAGCTAC 
AAGGAGGCCT TTGAGGAGAT GGAGGGGACC TCCCCAAGCA GCCCGCCACA CAGTGTGGCG 
CGCTCTCCTC CAGGTCTGGC CAAGACACCC CTGTCTGCTC TGGGCCTGAA ACCCCACAAC 
CCAGCAGACA TCTTGCTGCA CCCCACAGGT GTTGCCAGAA GACTGATCCA GCCAGAGGAA 
GACGAGGGGG AGGAGGTGAC TAAGCCTCCA GAAGAGCCCC GGAGCTATGT GGAGTCCGTG 
GCGAGGACAG CGGTGGCAGG GCCTCGAGCT CAGGACGTTG AGCCTAAGAG CTTCAGCGCA 
CCAGCTGCTC ACGCCTATGG GCACGAGACA CCCCTGAGGA ACGGGACCCC AGGGGGCTCC 
TTTGTCTCCC CGAGCCCCCT CTCCACAAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCC 
GTGGGCAGTT TCCCATCAGT GGTGAGCAGT GACCAGGGTC CCCGGACACC CTTCCAGCCT 
ATGCTGGACT CCAGCATCCG CTCAGGCAGC TTGGGGCAGC CGAGCCCTGC AGCACTGAGT 
TATCAGAGCT CCTCGCCTGT CCCTGTTGGT GGCAGCAGCT ACAACAGCCC TGACTACTCC 
CTCCAACCAT TCAGCTCTTC TCCAGAAAGC CAAGGCCAGC CACAGTACAG TGCGGCCAGT 
GTCCACATGG TACCTGGGAG CCCTCAGGCA CGACACAGGA CAGTGGGTAC CAACACTCCA 
CCTAGCCCTG GTTTTGGCCG GCGGGCCGTC AACCCCACCA TGGCTGCCCC TGGTAGTCCC 
AGTTTGAGCC ATCGGCAGGT GATGGGTCCA TCTGGTCCAG GTTTCCATGG TAACGTGGTT 
TCTGGCCACC CAGCCAGTGC AGCTACCACT CCTGGAAGCC CCAGCCTGGG CCGGCACCCA 
GTGGGAAGCC ATCAAGTTCC AGGCCTCCAC AGCAGTGTGG TCACCACTCC GGGAAGCCCA 
AGCCTGGGCC GACACCCTGG TGCCCACCAA GGGAATCTGG CCTCCAGTCT CCACAGCAAT 
GCAGTCATCA GCCCTGGGAG CCCAAGCTTG GGCCGGCACC TGGGTGGGTC TGGATCCGTG 
GTTCCTGGAA GCCCCAGCTT GGACCGGCAT GCAGCTTATG GTGGCTACTC TACCCCTGAG 
GACCGAAGAC CAACACTGTC CCGGCAAAGC AGTGCCTCTG GCTACCAGGC CCCATCCACA 
CCGTCATTCC CTGTCTCTCC TGCCTACTAC CCTGGCCTGA GCAGCCCTGC CACCTCACCC 
TCGCCAGACT CAGCAGCCTT CCGGCAGGGG AGCCCCACAC CAGCCTTACC AGAGAAGCGA 
AGAATGTCAG TGGGAGACCG GGCAGGCAGC CTCCCCAACT ATGCCACCAT CAATGGGAAA 
GTGTCTTCCT CGCCTGTCGC CAATGGCATG GCCAGTGGGA GCAGCACCGT CTCCTTCTCT 
CACACGCTTC CGGACTTTTC TAAGTATTCG ATGCCAGACA ATAGTCCGGA GACTCGGGCT 
AAAGTGAAGT TTGTCCAGGA CACTTCCAAG TATTGGTACA AGCCTGAGAT CTCCAGAGAA 
CAGGCCATCG CACTCCTAAA GGACCAGGAG CCAGGAGCCT TCATCATCCG GGACAGCCAC 
TCCTTCCGAG GGGCTTATGG GCTGGCCATG AAGGTGTCCT CACCCCCTCC AACCATCACT 
CAGCAGGGCA AGAAAGGAGA CATGACCCAT GAGCTGGTGA GGCACTTTCT GATCGAGACA 
GGCCCTAGAG GAGTGAAGCT CAAGGGTTGT CCCAATGAGC CAAACTTTGG TTCCCTTTCT 
GCCCTGGTCT ATCAGCACTC CGTCATCCCA CTGGCCCTGC CCTGTAAACT GGTCATTCCA 
AGCCGAGACC CCACAGATGA ATCAAAAGAT AGCTCAGGCC CTGCCAACTC AACCACTGAC 
CTGCTGAAGC AAGGAGCAGC TTGCAATGTG CTCTTCGTCA ACTCTGTGGA TATGGAGTCA 
CTCACCGGGC CACAGGCCAT TTCCAAAGCC ACATCTGAGA CTCTGGCTGC CGACCCCACA 
CCGGCTGCCA CCATTGTTCA CTTCAAGGTC TCTGCCCAAG GAATCACACT GACTGACAAT 
CAAAGAAAGC TCTTCTTTAG AAGACACTAC CCCCTCAACA CTGTCACCTT CTGTGACCTG 
GATCCACAAG AAAGAAAGTG GATGAAGACA GAGGGAGGCG CCCCTGCTAA GCTCTTTGGC 
TTCGTGGCCC GGAAGCAGGG CAGCACCACA GACAACGCTT GCCACCTCTT CGCAGAGCTT 
GACCCCAACC AGCCTGCGTC TGCCATCGTC AACTTCGTCT CCAAGGTCAT GCTGAGTGCT 
GGCCAGAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_011238476.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236778.1 Mus musculus tensin 1 (Tns1), transcript variant X24, mRNA.

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CloneID	OMu27365
Clone ID Related Accession (Same CDS sequence)	XM_006495880.2 , XM_011238476.2 , XM_006495880.3 , XM_011238477.1 , XM_006495881.3 , XM_006495877.3 , XM_006495881.2 , XM_011238476.1 , XM_006495877.4 , XM_006495879.1 , XM_006495878.3 , XM_006495882.3
Accession Version	XM_011238476.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238476.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236778.1
CDS	341..5647
Translation	MRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_011238476.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238476.2

ORF Insert Sequence:

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495880.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495943.1 Mus musculus tensin 1 (Tns1), transcript variant X25, mRNA.

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CloneID	OMu27365
Clone ID Related Accession (Same CDS sequence)	XM_006495880.2 , XM_011238476.2 , XM_006495880.3 , XM_011238477.1 , XM_006495881.3 , XM_006495877.3 , XM_006495881.2 , XM_011238476.1 , XM_006495877.4 , XM_006495879.1 , XM_006495878.3 , XM_006495882.3
Accession Version	XM_006495880.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495880.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495943.1
CDS	270..5576
Translation	MRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495880.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495880.3

ORF Insert Sequence:

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495881.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495944.1 Mus musculus tensin 1 (Tns1), transcript variant X29, mRNA.

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CloneID	OMu27365
Clone ID Related Accession (Same CDS sequence)	XM_006495880.2 , XM_011238476.2 , XM_006495880.3 , XM_011238477.1 , XM_006495881.3 , XM_006495877.3 , XM_006495881.2 , XM_011238476.1 , XM_006495877.4 , XM_006495879.1 , XM_006495878.3 , XM_006495882.3
Accession Version	XM_006495881.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495881.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495944.1
CDS	405..5711
Translation	MRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495881.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495881.3

ORF Insert Sequence:

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495877.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495940.1 Mus musculus tensin 1 (Tns1), transcript variant X22, mRNA.

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CloneID	OMu27365
Clone ID Related Accession (Same CDS sequence)	XM_006495880.2 , XM_011238476.2 , XM_006495880.3 , XM_011238477.1 , XM_006495881.3 , XM_006495877.3 , XM_006495881.2 , XM_011238476.1 , XM_006495877.4 , XM_006495879.1 , XM_006495878.3 , XM_006495882.3
Accession Version	XM_006495877.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006495877.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495940.1
CDS	356..5662
Translation	MRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495877.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495877.4

ORF Insert Sequence:

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_017319847.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175336.1 Mus musculus tensin 1 (Tns1), transcript variant X2, mRNA.

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CloneID	OMu77072
Clone ID Related Accession (Same CDS sequence)	XM_017319847.1
Accession Version	XM_017319847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGA AGTGGTGGGA CACACCCAAG GGCCGCTGGA TGGGAGCCTG 
TACGCCAAGG TGAAGAAGAA GGATTCCCTG AATGGCAGTT CGGGGCCTGT CACTACTGCA 
CGGCCTGCCC TGTCAGCCAC TCCCAACCAC GTGGAACACA CCCTGTCTGT GAGCAGCGAC 
TCTGGCAACT CCACAGCCTC CACTAAGACA GACAAGACCG ATGAGCCAGT CTCTGGGGCT 
ACCACTGCCC CTGCTGCCCT GAGTCCTCAG GAGAAGAAAG AGCTGGACCG CCTACTAAGT 
GGCTTTGGTG TGGATAGAGA AAAGCAAGGC GCCATGTACC GGGCTCAGCA ACTCAGATCC 
CATCCAGGCG GGGGCCCGAC TGTGCCCTCC CCTGGCCGCC ACATTGTCCC AGCCCAGGTT 
CACGTCAATG GTGGGGCCCT GGCATCTGAG CGGGAGACAG ACATTCTAGA TGATGAGCTG 
CCCATCCAGG ACGGGCAGAG TGGGGGCAGC ATGGGAACGC TGTCCTCCCT GGATGGGGTC 
ACCAACACCA GTGAAAGTGG CTACCCAGAG ACCCTGTCCC CACTGACCAA TGGCCTGGAC 
AAACCCTATT CCACAGAACC TGTGCTCAAC GGCGGAGGCT ACCCCTATGA GGCTGCCAAC 
CGGGTGATTC CTGTCCACAG CAGTCACTCG GCCCCCATTC GACCCTCCTA CTCTGCTCAG 
GAGGGTTTAG CTGGCTACCA GCGTGAGGGA CCCCACCCAG CCTGGTCACA GCAAGTGACC 
AGTGCCCACT GTGGCTGTGA CCCCAGTGGT CTATTCAGAT CCCAGTCCTT TCCAGATGTG 
GAGCCCCAGC TGCCCCAAGC TCCAACCCGT GGGGGTAGTA GCCGAGAGGC TGTGCAGAGG 
GGTCTGAATT CATGGCAACA GCAGCAGCCC CACCCACCTC CCCGCCAGCA GGAGAGATCC 
CCACTGCAGA GTCTTGCCCG CAGCAAGCCT AGCCCCCAGC TGTCAGCAGA GACCCCCGTA 
GCTGCTCTCC CAGAGTTCCC AAGGGCAGCG TCCCAGCAGG AGATCGAGCA GTCCATCGAA 
ACCCTCAATA TGTTGATGCT GGACCTGGAG CCAGCCTCCG CAGCTGCCCC TCTTCATAAG 
TCCCAGAGTG TCCCTGGGGC CTGGCCAGGG GCTTCTCCAC TCTCTTCCCA GCCCCTCTTG 
GGGTCTTCCC GCCAGTCCCA CCCACTTACC CAGTCCCGAT CTGGCTATAT CCCCAGTGGG 
CATTCTCTGG GAACCCCGGA GCTGGTCTCT TCTGGCAGGC CTTATTCACC TTACGATTAT 
CAGCTGCATC CAGCTGGATC CAACCAGAGT TTCCATCCAA AAAGTCCAGC CTCTTCCACC 
TTCCTTCCAA GCCCTCATAG CTCTGCGGGG CCTCAGGAGC CCCCAGCCTC TCTCCCTGGT 
CTCATCGCTC AGCCCCAGCT CCCACCAAAG GAAACTACAT CAGATCCCTC CCGAACTCCA 
GAGGAAGAGC CGCTGAACCT GGAAGGGCTG GTAGCCCATC GGGTAGCAGT GTACAATGCC 
AGACTACAGA GCCTGAGGCA GAGCCGCGGC TTCCAACCCT CTCCCCACTG GCGCCGATCT 
GCCTCCCTCT CTGGGGTTCA GGCTCGGGAG AGGCAGCCTG CAGAGCCCCC AGGCCCACTC 
CGGAGGCGGG CAGCCAGTGA TGGACAGTAT GAAAACCAGT CTCCGGAGGC CACATCCCCC 
CGTAGTCCTG GGGTCCGCTC CCCCGTCCAG TGCGTCTCCC CTGAGCTGGC TCTTACCATT 
GCCCTCAATC CTGGAGGGCG CCCCAAAGAG CCTCATTTGC ATAGCTACAA GGAGGCCTTT 
GAGGAGATGG AGGGGACCTC CCCAAGCAGC CCGCCACACA GTGTGGCGCG CTCTCCTCCA 
GGTCTGGCCA AGACACCCCT GTCTGCTCTG GGCCTGAAAC CCCACAACCC AGCAGACATC 
TTGCTGCACC CCACAGGTGT TGCCAGAAGA CTGATCCAGC CAGAGGAAGA CGAGGGGGAG 
GAGGTGACTA AGCCTCCAGA AGAGCCCCGG AGCTATGTGG AGTCCGTGGC GAGGACAGCG 
GTGGCAGGGC CTCGAGCTCA GGACGTTGAG CCTAAGAGCT TCAGCGCACC AGCTGCTCAC 
GCCTATGGGC ACGAGACACC CCTGAGGAAC GGGACCCCAG GGGGCTCCTT TGTCTCCCCG 
AGCCCCCTCT CCACAAGCAG CCCCATCCTC AGTGCTGACA GCACTTCCGT GGGCAGTTTC 
CCATCAGTGG TGAGCAGTGA CCAGGGTCCC CGGACACCCT TCCAGCCTAT GCTGGACTCC 
AGCATCCGCT CAGGCAGCTT GGGGCAGCCG AGCCCTGCAG CACTGAGTTA TCAGAGCTCC 
TCGCCTGTCC CTGTTGGTGG CAGCAGCTAC AACAGCCCTG ACTACTCCCT CCAACCATTC 
AGCTCTTCTC CAGAAAGCCA AGGCCAGCCA CAGTACAGTG CGGCCAGTGT CCACATGGTA 
CCTGGGAGCC CTCAGGCACG ACACAGGACA GTGGGTACCA ACACTCCACC TAGCCCTGGT 
TTTGGCCGGC GGGCCGTCAA CCCCACCATG GCTGCCCCTG GTAGTCCCAG TTTGAGCCAT 
CGGCAGGTGA TGGGTCCATC TGGTCCAGGT TTCCATGGTA ACGTGGTTTC TGGCCACCCA 
GCCAGTGCAG CTACCACTCC TGGAAGCCCC AGCCTGGGCC GGCACCCAGT GGGAAGCCAT 
CAAGTTCCAG GCCTCCACAG CAGTGTGGTC ACCACTCCGG GAAGCCCAAG CCTGGGCCGA 
CACCCTGGTG CCCACCAAGG GAATCTGGCC TCCAGTCTCC ACAGCAATGC AGTCATCAGC 
CCTGGGAGCC CAAGCTTGGG CCGGCACCTG GGTGGGTCTG GATCCGTGGT TCCTGGAAGC 
CCCAGCTTGG ACCGGCATGC AGCTTATGGT GGCTACTCTA CCCCTGAGGA CCGAAGACCA 
ACACTGTCCC GGCAAAGCAG TGCCTCTGGC TACCAGGCCC CATCCACACC GTCATTCCCT 
GTCTCTCCTG CCTACTACCC TGGCCTGAGC AGCCCTGCCA CCTCACCCTC GCCAGACTCA 
GCAGCCTTCC GGCAGGGGAG CCCCACACCA GCCTTACCAG AGAAGCGAAG AATGTCAGTG 
GGAGACCGGG CAGGCAGCCT CCCCAACTAT GCCACCATCA ATGGGAAAGT GTCTTCCTCG 
CCTGTCGCCA ATGGCATGGC CAGTGGGAGC AGCACCGTCT CCTTCTCTCA CACGCTTCCG 
GACTTTTCTA AGTATTCGAT GCCAGACAAT AGTCCGGAGA CTCGGGCTAA AGTGAAGTTT 
GTCCAGGACA CTTCCAAGTA TTGGTACAAG CCTGAGATCT CCAGAGAACA GGCCATCGCA 
CTCCTAAAGG ACCAGGAGCC AGGAGCCTTC ATCATCCGGG ACAGCCACTC CTTCCGAGGG 
GCTTATGGGC TGGCCATGAA GGTGTCCTCA CCCCCTCCAA CCATCACTCA GCAGGGCAAG 
AAAGGAGACA TGACCCATGA GCTGGTGAGG CACTTTCTGA TCGAGACAGG CCCTAGAGGA 
GTGAAGCTCA AGGGTTGTCC CAATGAGCCA AACTTTGGTT CCCTTTCTGC CCTGGTCTAT 
CAGCACTCCG TCATCCCACT GGCCCTGCCC TGTAAACTGG TCATTCCAAG CCGAGACCCC 
ACAGATGAAT CAAAAGATAG CTCAGGCCCT GCCAACTCAA CCACTGACCT GCTGAAGCAA 
GGAGCAGCTT GCAATGTGCT CTTCGTCAAC TCTGTGGATA TGGAGTCACT CACCGGGCCA 
CAGGCCATTT CCAAAGCCAC ATCTGAGACT CTGGCTGCCG ACCCCACACC GGCTGCCACC 
ATTGTTCACT TCAAGGTCTC TGCCCAAGGA ATCACACTGA CTGACAATCA AAGAAAGCTC 
TTCTTTAGAA GACACTACCC CCTCAACACT GTCACCTTCT GTGACCTGGA TCCACAAGAA 
AGAAAGTGGA TGAAGACAGA GGGAGGCGCC CCTGCTAAGC TCTTTGGCTT CGTGGCCCGG 
AAGCAGGGCA GCACCACAGA CAACGCTTGC CACCTCTTCG CAGAGCTTGA CCCCAACCAG 
CCTGCGTCTG CCATCGTCAA CTTCGTCTCC AAGGTCATGC TGAGTGCTGG CCAGAAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175336.1
CDS	16..5778
Translation	MLGDWGKGRGQPTGSPCPPWEHILFWNLGVSLPPCCSRESGVPWTQGALRRKLLVLPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEEVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_017319847.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319847.1

ORF Insert Sequence:

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGA AGTGGTGGGA CACACCCAAG GGCCGCTGGA TGGGAGCCTG 
TACGCCAAGG TGAAGAAGAA GGATTCCCTG AATGGCAGTT CGGGGCCTGT CACTACTGCA 
CGGCCTGCCC TGTCAGCCAC TCCCAACCAC GTGGAACACA CCCTGTCTGT GAGCAGCGAC 
TCTGGCAACT CCACAGCCTC CACTAAGACA GACAAGACCG ATGAGCCAGT CTCTGGGGCT 
ACCACTGCCC CTGCTGCCCT GAGTCCTCAG GAGAAGAAAG AGCTGGACCG CCTACTAAGT 
GGCTTTGGTG TGGATAGAGA AAAGCAAGGC GCCATGTACC GGGCTCAGCA ACTCAGATCC 
CATCCAGGCG GGGGCCCGAC TGTGCCCTCC CCTGGCCGCC ACATTGTCCC AGCCCAGGTT 
CACGTCAATG GTGGGGCCCT GGCATCTGAG CGGGAGACAG ACATTCTAGA TGATGAGCTG 
CCCATCCAGG ACGGGCAGAG TGGGGGCAGC ATGGGAACGC TGTCCTCCCT GGATGGGGTC 
ACCAACACCA GTGAAAGTGG CTACCCAGAG ACCCTGTCCC CACTGACCAA TGGCCTGGAC 
AAACCCTATT CCACAGAACC TGTGCTCAAC GGCGGAGGCT ACCCCTATGA GGCTGCCAAC 
CGGGTGATTC CTGTCCACAG CAGTCACTCG GCCCCCATTC GACCCTCCTA CTCTGCTCAG 
GAGGGTTTAG CTGGCTACCA GCGTGAGGGA CCCCACCCAG CCTGGTCACA GCAAGTGACC 
AGTGCCCACT GTGGCTGTGA CCCCAGTGGT CTATTCAGAT CCCAGTCCTT TCCAGATGTG 
GAGCCCCAGC TGCCCCAAGC TCCAACCCGT GGGGGTAGTA GCCGAGAGGC TGTGCAGAGG 
GGTCTGAATT CATGGCAACA GCAGCAGCCC CACCCACCTC CCCGCCAGCA GGAGAGATCC 
CCACTGCAGA GTCTTGCCCG CAGCAAGCCT AGCCCCCAGC TGTCAGCAGA GACCCCCGTA 
GCTGCTCTCC CAGAGTTCCC AAGGGCAGCG TCCCAGCAGG AGATCGAGCA GTCCATCGAA 
ACCCTCAATA TGTTGATGCT GGACCTGGAG CCAGCCTCCG CAGCTGCCCC TCTTCATAAG 
TCCCAGAGTG TCCCTGGGGC CTGGCCAGGG GCTTCTCCAC TCTCTTCCCA GCCCCTCTTG 
GGGTCTTCCC GCCAGTCCCA CCCACTTACC CAGTCCCGAT CTGGCTATAT CCCCAGTGGG 
CATTCTCTGG GAACCCCGGA GCTGGTCTCT TCTGGCAGGC CTTATTCACC TTACGATTAT 
CAGCTGCATC CAGCTGGATC CAACCAGAGT TTCCATCCAA AAAGTCCAGC CTCTTCCACC 
TTCCTTCCAA GCCCTCATAG CTCTGCGGGG CCTCAGGAGC CCCCAGCCTC TCTCCCTGGT 
CTCATCGCTC AGCCCCAGCT CCCACCAAAG GAAACTACAT CAGATCCCTC CCGAACTCCA 
GAGGAAGAGC CGCTGAACCT GGAAGGGCTG GTAGCCCATC GGGTAGCAGT GTACAATGCC 
AGACTACAGA GCCTGAGGCA GAGCCGCGGC TTCCAACCCT CTCCCCACTG GCGCCGATCT 
GCCTCCCTCT CTGGGGTTCA GGCTCGGGAG AGGCAGCCTG CAGAGCCCCC AGGCCCACTC 
CGGAGGCGGG CAGCCAGTGA TGGACAGTAT GAAAACCAGT CTCCGGAGGC CACATCCCCC 
CGTAGTCCTG GGGTCCGCTC CCCCGTCCAG TGCGTCTCCC CTGAGCTGGC TCTTACCATT 
GCCCTCAATC CTGGAGGGCG CCCCAAAGAG CCTCATTTGC ATAGCTACAA GGAGGCCTTT 
GAGGAGATGG AGGGGACCTC CCCAAGCAGC CCGCCACACA GTGTGGCGCG CTCTCCTCCA 
GGTCTGGCCA AGACACCCCT GTCTGCTCTG GGCCTGAAAC CCCACAACCC AGCAGACATC 
TTGCTGCACC CCACAGGTGT TGCCAGAAGA CTGATCCAGC CAGAGGAAGA CGAGGGGGAG 
GAGGTGACTA AGCCTCCAGA AGAGCCCCGG AGCTATGTGG AGTCCGTGGC GAGGACAGCG 
GTGGCAGGGC CTCGAGCTCA GGACGTTGAG CCTAAGAGCT TCAGCGCACC AGCTGCTCAC 
GCCTATGGGC ACGAGACACC CCTGAGGAAC GGGACCCCAG GGGGCTCCTT TGTCTCCCCG 
AGCCCCCTCT CCACAAGCAG CCCCATCCTC AGTGCTGACA GCACTTCCGT GGGCAGTTTC 
CCATCAGTGG TGAGCAGTGA CCAGGGTCCC CGGACACCCT TCCAGCCTAT GCTGGACTCC 
AGCATCCGCT CAGGCAGCTT GGGGCAGCCG AGCCCTGCAG CACTGAGTTA TCAGAGCTCC 
TCGCCTGTCC CTGTTGGTGG CAGCAGCTAC AACAGCCCTG ACTACTCCCT CCAACCATTC 
AGCTCTTCTC CAGAAAGCCA AGGCCAGCCA CAGTACAGTG CGGCCAGTGT CCACATGGTA 
CCTGGGAGCC CTCAGGCACG ACACAGGACA GTGGGTACCA ACACTCCACC TAGCCCTGGT 
TTTGGCCGGC GGGCCGTCAA CCCCACCATG GCTGCCCCTG GTAGTCCCAG TTTGAGCCAT 
CGGCAGGTGA TGGGTCCATC TGGTCCAGGT TTCCATGGTA ACGTGGTTTC TGGCCACCCA 
GCCAGTGCAG CTACCACTCC TGGAAGCCCC AGCCTGGGCC GGCACCCAGT GGGAAGCCAT 
CAAGTTCCAG GCCTCCACAG CAGTGTGGTC ACCACTCCGG GAAGCCCAAG CCTGGGCCGA 
CACCCTGGTG CCCACCAAGG GAATCTGGCC TCCAGTCTCC ACAGCAATGC AGTCATCAGC 
CCTGGGAGCC CAAGCTTGGG CCGGCACCTG GGTGGGTCTG GATCCGTGGT TCCTGGAAGC 
CCCAGCTTGG ACCGGCATGC AGCTTATGGT GGCTACTCTA CCCCTGAGGA CCGAAGACCA 
ACACTGTCCC GGCAAAGCAG TGCCTCTGGC TACCAGGCCC CATCCACACC GTCATTCCCT 
GTCTCTCCTG CCTACTACCC TGGCCTGAGC AGCCCTGCCA CCTCACCCTC GCCAGACTCA 
GCAGCCTTCC GGCAGGGGAG CCCCACACCA GCCTTACCAG AGAAGCGAAG AATGTCAGTG 
GGAGACCGGG CAGGCAGCCT CCCCAACTAT GCCACCATCA ATGGGAAAGT GTCTTCCTCG 
CCTGTCGCCA ATGGCATGGC CAGTGGGAGC AGCACCGTCT CCTTCTCTCA CACGCTTCCG 
GACTTTTCTA AGTATTCGAT GCCAGACAAT AGTCCGGAGA CTCGGGCTAA AGTGAAGTTT 
GTCCAGGACA CTTCCAAGTA TTGGTACAAG CCTGAGATCT CCAGAGAACA GGCCATCGCA 
CTCCTAAAGG ACCAGGAGCC AGGAGCCTTC ATCATCCGGG ACAGCCACTC CTTCCGAGGG 
GCTTATGGGC TGGCCATGAA GGTGTCCTCA CCCCCTCCAA CCATCACTCA GCAGGGCAAG 
AAAGGAGACA TGACCCATGA GCTGGTGAGG CACTTTCTGA TCGAGACAGG CCCTAGAGGA 
GTGAAGCTCA AGGGTTGTCC CAATGAGCCA AACTTTGGTT CCCTTTCTGC CCTGGTCTAT 
CAGCACTCCG TCATCCCACT GGCCCTGCCC TGTAAACTGG TCATTCCAAG CCGAGACCCC 
ACAGATGAAT CAAAAGATAG CTCAGGCCCT GCCAACTCAA CCACTGACCT GCTGAAGCAA 
GGAGCAGCTT GCAATGTGCT CTTCGTCAAC TCTGTGGATA TGGAGTCACT CACCGGGCCA 
CAGGCCATTT CCAAAGCCAC ATCTGAGACT CTGGCTGCCG ACCCCACACC GGCTGCCACC 
ATTGTTCACT TCAAGGTCTC TGCCCAAGGA ATCACACTGA CTGACAATCA AAGAAAGCTC 
TTCTTTAGAA GACACTACCC CCTCAACACT GTCACCTTCT GTGACCTGGA TCCACAAGAA 
AGAAAGTGGA TGAAGACAGA GGGAGGCGCC CCTGCTAAGC TCTTTGGCTT CGTGGCCCGG 
AAGCAGGGCA GCACCACAGA CAACGCTTGC CACCTCTTCG CAGAGCTTGA CCCCAACCAG 
CCTGCGTCTG CCATCGTCAA CTTCGTCTCC AAGGTCATGC TGAGTGCTGG CCAGAAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_017319848.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175337.1 Mus musculus tensin 1 (Tns1), transcript variant X3, mRNA.

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CloneID	OMu77073
Clone ID Related Accession (Same CDS sequence)	XM_017319848.1
Accession Version	XM_017319848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGTGTAC 
AATGCCAGAC TACAGAGCCT GAGGCAGAGC CGCGGCTTCC AACCCTCTCC CCACTGGCGC 
CGATCTGCCT CCCTCTCTGG GGTTCAGGCT CGGGAGAGGC AGCCTGCAGA GCCCCCAGGC 
CCACTCCGGA GGCGGGCAGC CAGTGATGGA CAGTATGAAA ACCAGTCTCC GGAGGCCACA 
TCCCCCCGTA GTCCTGGGGT CCGCTCCCCC GTCCAGTGCG TCTCCCCTGA GCTGGCTCTT 
ACCATTGCCC TCAATCCTGG AGGGCGCCCC AAAGAGCCTC ATTTGCATAG CTACAAGGAG 
GCCTTTGAGG AGATGGAGGG GACCTCCCCA AGCAGCCCGC CACACAGTGT GGCGCGCTCT 
CCTCCAGGTC TGGCCAAGAC ACCCCTGTCT GCTCTGGGCC TGAAACCCCA CAACCCAGCA 
GACATCTTGC TGCACCCCAC AGGTGAGGAA GACGAGGGGG AGGAGGTGAC TAAGCCTCCA 
GAAGAGCCCC GGAGCTATGT GGAGTCCGTG GCGAGGACAG CGGTGGCAGG GCCTCGAGCT 
CAGGACGTTG AGCCTAAGAG CTTCAGCGCA CCAGCTGCTC ACGCCTATGG GCACGAGACA 
CCCCTGAGGA ACGGGACCCC AGGGGGCTCC TTTGTCTCCC CGAGCCCCCT CTCCACAAGC 
AGCCCCATCC TCAGTGCTGA CAGCACTTCC GTGGGCAGTT TCCCATCAGT GGTGAGCAGT 
GACCAGGGTC CCCGGACACC CTTCCAGCCT ATGCTGGACT CCAGCATCCG CTCAGGCAGC 
TTGGGGCAGC CGAGCCCTGC AGCACTGAGT TATCAGAGCT CCTCGCCTGT CCCTGTTGGT 
GGCAGCAGCT ACAACAGCCC TGACTACTCC CTCCAACCAT TCAGCTCTTC TCCAGAAAGC 
CAAGGCCAGC CACAGTACAG TGCGGCCAGT GTCCACATGG TACCTGGGAG CCCTCAGGCA 
CGACACAGGA CAGTGGGTAC CAACACTCCA CCTAGCCCTG GTTTTGGCCG GCGGGCCGTC 
AACCCCACCA TGGCTGCCCC TGGTAGTCCC AGTTTGAGCC ATCGGCAGGT GATGGGTCCA 
TCTGGTCCAG GTTTCCATGG TAACGTGGTT TCTGGCCACC CAGCCAGTGC AGCTACCACT 
CCTGGAAGCC CCAGCCTGGG CCGGCACCCA GTGGGAAGCC ATCAAGTTCC AGGCCTCCAC 
AGCAGTGTGG TCACCACTCC GGGAAGCCCA AGCCTGGGCC GACACCCTGG TGCCCACCAA 
GGGAATCTGG CCTCCAGTCT CCACAGCAAT GCAGTCATCA GCCCTGGGAG CCCAAGCTTG 
GGCCGGCACC TGGGTGGGTC TGGATCCGTG GTTCCTGGAA GCCCCAGCTT GGACCGGCAT 
GCAGCTTATG GTGGCTACTC TACCCCTGAG GACCGAAGAC CAACACTGTC CCGGCAAAGC 
AGTGCCTCTG GCTACCAGGC CCCATCCACA CCGTCATTCC CTGTCTCTCC TGCCTACTAC 
CCTGGCCTGA GCAGCCCTGC CACCTCACCC TCGCCAGACT CAGCAGCCTT CCGGCAGGGG 
AGCCCCACAC CAGCCTTACC AGAGAAGCGA AGAATGTCAG TGGGAGACCG GGCAGGCAGC 
CTCCCCAACT ATGCCACCAT CAATGGGAAA GTGTCTTCCT CGCCTGTCGC CAATGGCATG 
GCCAGTGGGA GCAGCACCGT CTCCTTCTCT CACACGCTTC CGGACTTTTC TAAGTATTCG 
ATGCCAGACA ATAGTCCGGA GACTCGGGCT AAAGTGAAGT TTGTCCAGGA CACTTCCAAG 
TATTGGTACA AGCCTGAGAT CTCCAGAGAA CAGGCCATCG CACTCCTAAA GGACCAGGAG 
CCAGGAGCCT TCATCATCCG GGACAGCCAC TCCTTCCGAG GGGCTTATGG GCTGGCCATG 
AAGGTGTCCT CACCCCCTCC AACCATCACT CAGCAGGGCA AGAAAGGAGA CATGACCCAT 
GAGCTGGTGA GGCACTTTCT GATCGAGACA GGCCCTAGAG GAGTGAAGCT CAAGGGTTGT 
CCCAATGAGC CAAACTTTGG TTCCCTTTCT GCCCTGGTCT ATCAGCACTC CGTCATCCCA 
CTGGCCCTGC CCTGTAAACT GGTCATTCCA AGCCGAGACC CCACAGATGA ATCAAAAGAT 
AGCTCAGGCC CTGCCAACTC AACCACTGAC CTGCTGAAGC AAGGAGCAGC TTGCAATGTG 
CTCTTCGTCA ACTCTGTGGA TATGGAGTCA CTCACCGGGC CACAGGCCAT TTCCAAAGCC 
ACATCTGAGA CTCTGGCTGC CGACCCCACA CCGGCTGCCA CCATTGTTCA CTTCAAGGTC 
TCTGCCCAAG GAATCACACT GACTGACAAT CAAAGAAAGC TCTTCTTTAG AAGACACTAC 
CCCCTCAACA CTGTCACCTT CTGTGACCTG GATCCACAAG AAAGAAAGTG GATGAAGACA 
GAGGGAGGCG CCCCTGCTAA GCTCTTTGGC TTCGTGGCCC GGAAGCAGGG CAGCACCACA 
GACAACGCTT GCCACCTCTT CGCAGAGCTT GACCCCAACC AGCCTGCGTC TGCCATCGTC 
AACTTCGTCT CCAAGGTCAT GCTGAGTGCT GGCCAGAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175337.1
CDS	16..5760
Translation	MLGDWGKGRGQPTGSPCPPWEHILFWNLGVSLPPCCSRESGVPWTQGALRRKLLVLPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_017319848.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319848.1

ORF Insert Sequence:

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGTGTAC 
AATGCCAGAC TACAGAGCCT GAGGCAGAGC CGCGGCTTCC AACCCTCTCC CCACTGGCGC 
CGATCTGCCT CCCTCTCTGG GGTTCAGGCT CGGGAGAGGC AGCCTGCAGA GCCCCCAGGC 
CCACTCCGGA GGCGGGCAGC CAGTGATGGA CAGTATGAAA ACCAGTCTCC GGAGGCCACA 
TCCCCCCGTA GTCCTGGGGT CCGCTCCCCC GTCCAGTGCG TCTCCCCTGA GCTGGCTCTT 
ACCATTGCCC TCAATCCTGG AGGGCGCCCC AAAGAGCCTC ATTTGCATAG CTACAAGGAG 
GCCTTTGAGG AGATGGAGGG GACCTCCCCA AGCAGCCCGC CACACAGTGT GGCGCGCTCT 
CCTCCAGGTC TGGCCAAGAC ACCCCTGTCT GCTCTGGGCC TGAAACCCCA CAACCCAGCA 
GACATCTTGC TGCACCCCAC AGGTGAGGAA GACGAGGGGG AGGAGGTGAC TAAGCCTCCA 
GAAGAGCCCC GGAGCTATGT GGAGTCCGTG GCGAGGACAG CGGTGGCAGG GCCTCGAGCT 
CAGGACGTTG AGCCTAAGAG CTTCAGCGCA CCAGCTGCTC ACGCCTATGG GCACGAGACA 
CCCCTGAGGA ACGGGACCCC AGGGGGCTCC TTTGTCTCCC CGAGCCCCCT CTCCACAAGC 
AGCCCCATCC TCAGTGCTGA CAGCACTTCC GTGGGCAGTT TCCCATCAGT GGTGAGCAGT 
GACCAGGGTC CCCGGACACC CTTCCAGCCT ATGCTGGACT CCAGCATCCG CTCAGGCAGC 
TTGGGGCAGC CGAGCCCTGC AGCACTGAGT TATCAGAGCT CCTCGCCTGT CCCTGTTGGT 
GGCAGCAGCT ACAACAGCCC TGACTACTCC CTCCAACCAT TCAGCTCTTC TCCAGAAAGC 
CAAGGCCAGC CACAGTACAG TGCGGCCAGT GTCCACATGG TACCTGGGAG CCCTCAGGCA 
CGACACAGGA CAGTGGGTAC CAACACTCCA CCTAGCCCTG GTTTTGGCCG GCGGGCCGTC 
AACCCCACCA TGGCTGCCCC TGGTAGTCCC AGTTTGAGCC ATCGGCAGGT GATGGGTCCA 
TCTGGTCCAG GTTTCCATGG TAACGTGGTT TCTGGCCACC CAGCCAGTGC AGCTACCACT 
CCTGGAAGCC CCAGCCTGGG CCGGCACCCA GTGGGAAGCC ATCAAGTTCC AGGCCTCCAC 
AGCAGTGTGG TCACCACTCC GGGAAGCCCA AGCCTGGGCC GACACCCTGG TGCCCACCAA 
GGGAATCTGG CCTCCAGTCT CCACAGCAAT GCAGTCATCA GCCCTGGGAG CCCAAGCTTG 
GGCCGGCACC TGGGTGGGTC TGGATCCGTG GTTCCTGGAA GCCCCAGCTT GGACCGGCAT 
GCAGCTTATG GTGGCTACTC TACCCCTGAG GACCGAAGAC CAACACTGTC CCGGCAAAGC 
AGTGCCTCTG GCTACCAGGC CCCATCCACA CCGTCATTCC CTGTCTCTCC TGCCTACTAC 
CCTGGCCTGA GCAGCCCTGC CACCTCACCC TCGCCAGACT CAGCAGCCTT CCGGCAGGGG 
AGCCCCACAC CAGCCTTACC AGAGAAGCGA AGAATGTCAG TGGGAGACCG GGCAGGCAGC 
CTCCCCAACT ATGCCACCAT CAATGGGAAA GTGTCTTCCT CGCCTGTCGC CAATGGCATG 
GCCAGTGGGA GCAGCACCGT CTCCTTCTCT CACACGCTTC CGGACTTTTC TAAGTATTCG 
ATGCCAGACA ATAGTCCGGA GACTCGGGCT AAAGTGAAGT TTGTCCAGGA CACTTCCAAG 
TATTGGTACA AGCCTGAGAT CTCCAGAGAA CAGGCCATCG CACTCCTAAA GGACCAGGAG 
CCAGGAGCCT TCATCATCCG GGACAGCCAC TCCTTCCGAG GGGCTTATGG GCTGGCCATG 
AAGGTGTCCT CACCCCCTCC AACCATCACT CAGCAGGGCA AGAAAGGAGA CATGACCCAT 
GAGCTGGTGA GGCACTTTCT GATCGAGACA GGCCCTAGAG GAGTGAAGCT CAAGGGTTGT 
CCCAATGAGC CAAACTTTGG TTCCCTTTCT GCCCTGGTCT ATCAGCACTC CGTCATCCCA 
CTGGCCCTGC CCTGTAAACT GGTCATTCCA AGCCGAGACC CCACAGATGA ATCAAAAGAT 
AGCTCAGGCC CTGCCAACTC AACCACTGAC CTGCTGAAGC AAGGAGCAGC TTGCAATGTG 
CTCTTCGTCA ACTCTGTGGA TATGGAGTCA CTCACCGGGC CACAGGCCAT TTCCAAAGCC 
ACATCTGAGA CTCTGGCTGC CGACCCCACA CCGGCTGCCA CCATTGTTCA CTTCAAGGTC 
TCTGCCCAAG GAATCACACT GACTGACAAT CAAAGAAAGC TCTTCTTTAG AAGACACTAC 
CCCCTCAACA CTGTCACCTT CTGTGACCTG GATCCACAAG AAAGAAAGTG GATGAAGACA 
GAGGGAGGCG CCCCTGCTAA GCTCTTTGGC TTCGTGGCCC GGAAGCAGGG CAGCACCACA 
GACAACGCTT GCCACCTCTT CGCAGAGCTT GACCCCAACC AGCCTGCGTC TGCCATCGTC 
AACTTCGTCT CCAAGGTCAT GCTGAGTGCT GGCCAGAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_017319853.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175342.1 Mus musculus tensin 1 (Tns1), transcript variant X4, mRNA.

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CloneID	OMu77074
Clone ID Related Accession (Same CDS sequence)	XM_017319853.1
Accession Version	XM_017319853.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGTGTAC 
AATGCCAGAC TACAGAGCCT GAGGCAGAGC CGCGGCTTCC AACCCTCTCC CCACTGGCGC 
CGATCTGCCT CCCTCTCTGG GGTTCAGGCT CGGGAGAGGC AGCCTGCAGA GCCCCCAGGC 
CCACTCCGGA GGCGGGCAGC CAGTGATGGA CAGTATGAAA ACCAGTCTCC GGAGGCCACA 
TCCCCCCGTA GTCCTGGGGT CCGCTCCCCC GTCCAGTGCG TCTCCCCTGA GCTGGCTCTT 
ACCATTGCCC TCAATCCTGG AGGGCGCCCC AAAGAGCCTC ATTTGCATAG CTACAAGGAG 
GCCTTTGAGG AGATGGAGGG GACCTCCCCA AGCAGCCCGC CACACAGTGT GGCGCGCTCT 
CCTCCAGGTC TGGCCAAGAC ACCCCTGTCT GCTCTGGGCC TGAAACCCCA CAACCCAGCA 
GACATCTTGC TGCACCCCAC AGGTGTTGCC AGAAGACTGA TCCAGCCAGA GCCCCGGAGC 
TATGTGGAGT CCGTGGCGAG GACAGCGGTG GCAGGGCCTC GAGCTCAGGA CGTTGAGCCT 
AAGAGCTTCA GCGCACCAGC TGCTCACGCC TATGGGCACG AGACACCCCT GAGGAACGGG 
ACCCCAGGGG GCTCCTTTGT CTCCCCGAGC CCCCTCTCCA CAAGCAGCCC CATCCTCAGT 
GCTGACAGCA CTTCCGTGGG CAGTTTCCCA TCAGTGGTGA GCAGTGACCA GGGTCCCCGG 
ACACCCTTCC AGCCTATGCT GGACTCCAGC ATCCGCTCAG GCAGCTTGGG GCAGCCGAGC 
CCTGCAGCAC TGAGTTATCA GAGCTCCTCG CCTGTCCCTG TTGGTGGCAG CAGCTACAAC 
AGCCCTGACT ACTCCCTCCA ACCATTCAGC TCTTCTCCAG AAAGCCAAGG CCAGCCACAG 
TACAGTGCGG CCAGTGTCCA CATGGTACCT GGGAGCCCTC AGGCACGACA CAGGACAGTG 
GGTACCAACA CTCCACCTAG CCCTGGTTTT GGCCGGCGGG CCGTCAACCC CACCATGGCT 
GCCCCTGGTA GTCCCAGTTT GAGCCATCGG CAGGTGATGG GTCCATCTGG TCCAGGTTTC 
CATGGTAACG TGGTTTCTGG CCACCCAGCC AGTGCAGCTA CCACTCCTGG AAGCCCCAGC 
CTGGGCCGGC ACCCAGTGGG AAGCCATCAA GTTCCAGGCC TCCACAGCAG TGTGGTCACC 
ACTCCGGGAA GCCCAAGCCT GGGCCGACAC CCTGGTGCCC ACCAAGGGAA TCTGGCCTCC 
AGTCTCCACA GCAATGCAGT CATCAGCCCT GGGAGCCCAA GCTTGGGCCG GCACCTGGGT 
GGGTCTGGAT CCGTGGTTCC TGGAAGCCCC AGCTTGGACC GGCATGCAGC TTATGGTGGC 
TACTCTACCC CTGAGGACCG AAGACCAACA CTGTCCCGGC AAAGCAGTGC CTCTGGCTAC 
CAGGCCCCAT CCACACCGTC ATTCCCTGTC TCTCCTGCCT ACTACCCTGG CCTGAGCAGC 
CCTGCCACCT CACCCTCGCC AGACTCAGCA GCCTTCCGGC AGGGGAGCCC CACACCAGCC 
TTACCAGAGA AGCGAAGAAT GTCAGTGGGA GACCGGGCAG GCAGCCTCCC CAACTATGCC 
ACCATCAATG GGAAAGTGTC TTCCTCGCCT GTCGCCAATG GCATGGCCAG TGGGAGCAGC 
ACCGTCTCCT TCTCTCACAC GCTTCCGGAC TTTTCTAAGT ATTCGATGCC AGACAATAGT 
CCGGAGACTC GGGCTAAAGT GAAGTTTGTC CAGGACACTT CCAAGTATTG GTACAAGCCT 
GAGATCTCCA GAGAACAGGC CATCGCACTC CTAAAGGACC AGGAGCCAGG AGCCTTCATC 
ATCCGGGACA GCCACTCCTT CCGAGGGGCT TATGGGCTGG CCATGAAGGT GTCCTCACCC 
CCTCCAACCA TCACTCAGCA GGGCAAGAAA GGAGACATGA CCCATGAGCT GGTGAGGCAC 
TTTCTGATCG AGACAGGCCC TAGAGGAGTG AAGCTCAAGG GTTGTCCCAA TGAGCCAAAC 
TTTGGTTCCC TTTCTGCCCT GGTCTATCAG CACTCCGTCA TCCCACTGGC CCTGCCCTGT 
AAACTGGTCA TTCCAAGCCG AGACCCCACA GATGAATCAA AAGATAGCTC AGGCCCTGCC 
AACTCAACCA CTGACCTGCT GAAGCAAGGA GCAGCTTGCA ATGTGCTCTT CGTCAACTCT 
GTGGATATGG AGTCACTCAC CGGGCCACAG GCCATTTCCA AAGCCACATC TGAGACTCTG 
GCTGCCGACC CCACACCGGC TGCCACCATT GTTCACTTCA AGGTCTCTGC CCAAGGAATC 
ACACTGACTG ACAATCAAAG AAAGCTCTTC TTTAGAAGAC ACTACCCCCT CAACACTGTC 
ACCTTCTGTG ACCTGGATCC ACAAGAAAGA AAGTGGATGA AGACAGAGGG AGGCGCCCCT 
GCTAAGCTCT TTGGCTTCGT GGCCCGGAAG CAGGGCAGCA CCACAGACAA CGCTTGCCAC 
CTCTTCGCAG AGCTTGACCC CAACCAGCCT GCGTCTGCCA TCGTCAACTT CGTCTCCAAG 
GTCATGCTGA GTGCTGGCCA GAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175342.1
CDS	16..5745
Translation	MLGDWGKGRGQPTGSPCPPWEHILFWNLGVSLPPCCSRESGVPWTQGALRRKLLVLPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_017319853.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319853.1

ORF Insert Sequence:

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGTGTAC 
AATGCCAGAC TACAGAGCCT GAGGCAGAGC CGCGGCTTCC AACCCTCTCC CCACTGGCGC 
CGATCTGCCT CCCTCTCTGG GGTTCAGGCT CGGGAGAGGC AGCCTGCAGA GCCCCCAGGC 
CCACTCCGGA GGCGGGCAGC CAGTGATGGA CAGTATGAAA ACCAGTCTCC GGAGGCCACA 
TCCCCCCGTA GTCCTGGGGT CCGCTCCCCC GTCCAGTGCG TCTCCCCTGA GCTGGCTCTT 
ACCATTGCCC TCAATCCTGG AGGGCGCCCC AAAGAGCCTC ATTTGCATAG CTACAAGGAG 
GCCTTTGAGG AGATGGAGGG GACCTCCCCA AGCAGCCCGC CACACAGTGT GGCGCGCTCT 
CCTCCAGGTC TGGCCAAGAC ACCCCTGTCT GCTCTGGGCC TGAAACCCCA CAACCCAGCA 
GACATCTTGC TGCACCCCAC AGGTGTTGCC AGAAGACTGA TCCAGCCAGA GCCCCGGAGC 
TATGTGGAGT CCGTGGCGAG GACAGCGGTG GCAGGGCCTC GAGCTCAGGA CGTTGAGCCT 
AAGAGCTTCA GCGCACCAGC TGCTCACGCC TATGGGCACG AGACACCCCT GAGGAACGGG 
ACCCCAGGGG GCTCCTTTGT CTCCCCGAGC CCCCTCTCCA CAAGCAGCCC CATCCTCAGT 
GCTGACAGCA CTTCCGTGGG CAGTTTCCCA TCAGTGGTGA GCAGTGACCA GGGTCCCCGG 
ACACCCTTCC AGCCTATGCT GGACTCCAGC ATCCGCTCAG GCAGCTTGGG GCAGCCGAGC 
CCTGCAGCAC TGAGTTATCA GAGCTCCTCG CCTGTCCCTG TTGGTGGCAG CAGCTACAAC 
AGCCCTGACT ACTCCCTCCA ACCATTCAGC TCTTCTCCAG AAAGCCAAGG CCAGCCACAG 
TACAGTGCGG CCAGTGTCCA CATGGTACCT GGGAGCCCTC AGGCACGACA CAGGACAGTG 
GGTACCAACA CTCCACCTAG CCCTGGTTTT GGCCGGCGGG CCGTCAACCC CACCATGGCT 
GCCCCTGGTA GTCCCAGTTT GAGCCATCGG CAGGTGATGG GTCCATCTGG TCCAGGTTTC 
CATGGTAACG TGGTTTCTGG CCACCCAGCC AGTGCAGCTA CCACTCCTGG AAGCCCCAGC 
CTGGGCCGGC ACCCAGTGGG AAGCCATCAA GTTCCAGGCC TCCACAGCAG TGTGGTCACC 
ACTCCGGGAA GCCCAAGCCT GGGCCGACAC CCTGGTGCCC ACCAAGGGAA TCTGGCCTCC 
AGTCTCCACA GCAATGCAGT CATCAGCCCT GGGAGCCCAA GCTTGGGCCG GCACCTGGGT 
GGGTCTGGAT CCGTGGTTCC TGGAAGCCCC AGCTTGGACC GGCATGCAGC TTATGGTGGC 
TACTCTACCC CTGAGGACCG AAGACCAACA CTGTCCCGGC AAAGCAGTGC CTCTGGCTAC 
CAGGCCCCAT CCACACCGTC ATTCCCTGTC TCTCCTGCCT ACTACCCTGG CCTGAGCAGC 
CCTGCCACCT CACCCTCGCC AGACTCAGCA GCCTTCCGGC AGGGGAGCCC CACACCAGCC 
TTACCAGAGA AGCGAAGAAT GTCAGTGGGA GACCGGGCAG GCAGCCTCCC CAACTATGCC 
ACCATCAATG GGAAAGTGTC TTCCTCGCCT GTCGCCAATG GCATGGCCAG TGGGAGCAGC 
ACCGTCTCCT TCTCTCACAC GCTTCCGGAC TTTTCTAAGT ATTCGATGCC AGACAATAGT 
CCGGAGACTC GGGCTAAAGT GAAGTTTGTC CAGGACACTT CCAAGTATTG GTACAAGCCT 
GAGATCTCCA GAGAACAGGC CATCGCACTC CTAAAGGACC AGGAGCCAGG AGCCTTCATC 
ATCCGGGACA GCCACTCCTT CCGAGGGGCT TATGGGCTGG CCATGAAGGT GTCCTCACCC 
CCTCCAACCA TCACTCAGCA GGGCAAGAAA GGAGACATGA CCCATGAGCT GGTGAGGCAC 
TTTCTGATCG AGACAGGCCC TAGAGGAGTG AAGCTCAAGG GTTGTCCCAA TGAGCCAAAC 
TTTGGTTCCC TTTCTGCCCT GGTCTATCAG CACTCCGTCA TCCCACTGGC CCTGCCCTGT 
AAACTGGTCA TTCCAAGCCG AGACCCCACA GATGAATCAA AAGATAGCTC AGGCCCTGCC 
AACTCAACCA CTGACCTGCT GAAGCAAGGA GCAGCTTGCA ATGTGCTCTT CGTCAACTCT 
GTGGATATGG AGTCACTCAC CGGGCCACAG GCCATTTCCA AAGCCACATC TGAGACTCTG 
GCTGCCGACC CCACACCGGC TGCCACCATT GTTCACTTCA AGGTCTCTGC CCAAGGAATC 
ACACTGACTG ACAATCAAAG AAAGCTCTTC TTTAGAAGAC ACTACCCCCT CAACACTGTC 
ACCTTCTGTG ACCTGGATCC ACAAGAAAGA AAGTGGATGA AGACAGAGGG AGGCGCCCCT 
GCTAAGCTCT TTGGCTTCGT GGCCCGGAAG CAGGGCAGCA CCACAGACAA CGCTTGCCAC 
CTCTTCGCAG AGCTTGACCC CAACCAGCCT GCGTCTGCCA TCGTCAACTT CGTCTCCAAG 
GTCATGCTGA GTGCTGGCCA GAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_017319856.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175345.1 Mus musculus tensin 1 (Tns1), transcript variant X5, mRNA.

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CloneID	OMu77075
Clone ID Related Accession (Same CDS sequence)	XM_017319856.1
Accession Version	XM_017319856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGTGTAC 
AATGCCAGAC TACAGAGCCT GAGGCAGAGC CGCGGCTTCC AACCCTCTCC CCACTGGCGC 
CGATCTGCCT CCCTCTCTGG GGTTCAGGCT CGGGAGAGGC AGCCTGCAGA GCCCCCAGGC 
CCACTCCGGA GGCGGGCAGC CAGTGATGGA CAGTATGAAA ACCAGTCTCC GGAGGCCACA 
TCCCCCCGTA GTCCTGGGGT CCGCTCCCCC GTCCAGTGCG TCTCCCCTGA GCTGGCTCTT 
ACCATTGCCC TCAATCCTGG AGGGCGCCCC AAAGAGCCTC ATTTGCATAG CTACAAGGAG 
GCCTTTGAGG AGATGGAGGG GACCTCCCCA AGCAGCCCGC CACACAGTGT GGCGCGCTCT 
CCTCCAGGTC TGGCCAAGAC ACCCCTGTCT GCTCTGGGCC TGAAACCCCA CAACCCAGCA 
GACATCTTGC TGCACCCCAC AGGTGAGCCC CGGAGCTATG TGGAGTCCGT GGCGAGGACA 
GCGGTGGCAG GGCCTCGAGC TCAGGACGTT GAGCCTAAGA GCTTCAGCGC ACCAGCTGCT 
CACGCCTATG GGCACGAGAC ACCCCTGAGG AACGGGACCC CAGGGGGCTC CTTTGTCTCC 
CCGAGCCCCC TCTCCACAAG CAGCCCCATC CTCAGTGCTG ACAGCACTTC CGTGGGCAGT 
TTCCCATCAG TGGTGAGCAG TGACCAGGGT CCCCGGACAC CCTTCCAGCC TATGCTGGAC 
TCCAGCATCC GCTCAGGCAG CTTGGGGCAG CCGAGCCCTG CAGCACTGAG TTATCAGAGC 
TCCTCGCCTG TCCCTGTTGG TGGCAGCAGC TACAACAGCC CTGACTACTC CCTCCAACCA 
TTCAGCTCTT CTCCAGAAAG CCAAGGCCAG CCACAGTACA GTGCGGCCAG TGTCCACATG 
GTACCTGGGA GCCCTCAGGC ACGACACAGG ACAGTGGGTA CCAACACTCC ACCTAGCCCT 
GGTTTTGGCC GGCGGGCCGT CAACCCCACC ATGGCTGCCC CTGGTAGTCC CAGTTTGAGC 
CATCGGCAGG TGATGGGTCC ATCTGGTCCA GGTTTCCATG GTAACGTGGT TTCTGGCCAC 
CCAGCCAGTG CAGCTACCAC TCCTGGAAGC CCCAGCCTGG GCCGGCACCC AGTGGGAAGC 
CATCAAGTTC CAGGCCTCCA CAGCAGTGTG GTCACCACTC CGGGAAGCCC AAGCCTGGGC 
CGACACCCTG GTGCCCACCA AGGGAATCTG GCCTCCAGTC TCCACAGCAA TGCAGTCATC 
AGCCCTGGGA GCCCAAGCTT GGGCCGGCAC CTGGGTGGGT CTGGATCCGT GGTTCCTGGA 
AGCCCCAGCT TGGACCGGCA TGCAGCTTAT GGTGGCTACT CTACCCCTGA GGACCGAAGA 
CCAACACTGT CCCGGCAAAG CAGTGCCTCT GGCTACCAGG CCCCATCCAC ACCGTCATTC 
CCTGTCTCTC CTGCCTACTA CCCTGGCCTG AGCAGCCCTG CCACCTCACC CTCGCCAGAC 
TCAGCAGCCT TCCGGCAGGG GAGCCCCACA CCAGCCTTAC CAGAGAAGCG AAGAATGTCA 
GTGGGAGACC GGGCAGGCAG CCTCCCCAAC TATGCCACCA TCAATGGGAA AGTGTCTTCC 
TCGCCTGTCG CCAATGGCAT GGCCAGTGGG AGCAGCACCG TCTCCTTCTC TCACACGCTT 
CCGGACTTTT CTAAGTATTC GATGCCAGAC AATAGTCCGG AGACTCGGGC TAAAGTGAAG 
TTTGTCCAGG ACACTTCCAA GTATTGGTAC AAGCCTGAGA TCTCCAGAGA ACAGGCCATC 
GCACTCCTAA AGGACCAGGA GCCAGGAGCC TTCATCATCC GGGACAGCCA CTCCTTCCGA 
GGGGCTTATG GGCTGGCCAT GAAGGTGTCC TCACCCCCTC CAACCATCAC TCAGCAGGGC 
AAGAAAGGAG ACATGACCCA TGAGCTGGTG AGGCACTTTC TGATCGAGAC AGGCCCTAGA 
GGAGTGAAGC TCAAGGGTTG TCCCAATGAG CCAAACTTTG GTTCCCTTTC TGCCCTGGTC 
TATCAGCACT CCGTCATCCC ACTGGCCCTG CCCTGTAAAC TGGTCATTCC AAGCCGAGAC 
CCCACAGATG AATCAAAAGA TAGCTCAGGC CCTGCCAACT CAACCACTGA CCTGCTGAAG 
CAAGGAGCAG CTTGCAATGT GCTCTTCGTC AACTCTGTGG ATATGGAGTC ACTCACCGGG 
CCACAGGCCA TTTCCAAAGC CACATCTGAG ACTCTGGCTG CCGACCCCAC ACCGGCTGCC 
ACCATTGTTC ACTTCAAGGT CTCTGCCCAA GGAATCACAC TGACTGACAA TCAAAGAAAG 
CTCTTCTTTA GAAGACACTA CCCCCTCAAC ACTGTCACCT TCTGTGACCT GGATCCACAA 
GAAAGAAAGT GGATGAAGAC AGAGGGAGGC GCCCCTGCTA AGCTCTTTGG CTTCGTGGCC 
CGGAAGCAGG GCAGCACCAC AGACAACGCT TGCCACCTCT TCGCAGAGCT TGACCCCAAC 
CAGCCTGCGT CTGCCATCGT CAACTTCGTC TCCAAGGTCA TGCTGAGTGC TGGCCAGAAG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175345.1
CDS	16..5721
Translation	MLGDWGKGRGQPTGSPCPPWEHILFWNLGVSLPPCCSRESGVPWTQGALRRKLLVLPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_017319856.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319856.1

ORF Insert Sequence:

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGTGTAC 
AATGCCAGAC TACAGAGCCT GAGGCAGAGC CGCGGCTTCC AACCCTCTCC CCACTGGCGC 
CGATCTGCCT CCCTCTCTGG GGTTCAGGCT CGGGAGAGGC AGCCTGCAGA GCCCCCAGGC 
CCACTCCGGA GGCGGGCAGC CAGTGATGGA CAGTATGAAA ACCAGTCTCC GGAGGCCACA 
TCCCCCCGTA GTCCTGGGGT CCGCTCCCCC GTCCAGTGCG TCTCCCCTGA GCTGGCTCTT 
ACCATTGCCC TCAATCCTGG AGGGCGCCCC AAAGAGCCTC ATTTGCATAG CTACAAGGAG 
GCCTTTGAGG AGATGGAGGG GACCTCCCCA AGCAGCCCGC CACACAGTGT GGCGCGCTCT 
CCTCCAGGTC TGGCCAAGAC ACCCCTGTCT GCTCTGGGCC TGAAACCCCA CAACCCAGCA 
GACATCTTGC TGCACCCCAC AGGTGAGCCC CGGAGCTATG TGGAGTCCGT GGCGAGGACA 
GCGGTGGCAG GGCCTCGAGC TCAGGACGTT GAGCCTAAGA GCTTCAGCGC ACCAGCTGCT 
CACGCCTATG GGCACGAGAC ACCCCTGAGG AACGGGACCC CAGGGGGCTC CTTTGTCTCC 
CCGAGCCCCC TCTCCACAAG CAGCCCCATC CTCAGTGCTG ACAGCACTTC CGTGGGCAGT 
TTCCCATCAG TGGTGAGCAG TGACCAGGGT CCCCGGACAC CCTTCCAGCC TATGCTGGAC 
TCCAGCATCC GCTCAGGCAG CTTGGGGCAG CCGAGCCCTG CAGCACTGAG TTATCAGAGC 
TCCTCGCCTG TCCCTGTTGG TGGCAGCAGC TACAACAGCC CTGACTACTC CCTCCAACCA 
TTCAGCTCTT CTCCAGAAAG CCAAGGCCAG CCACAGTACA GTGCGGCCAG TGTCCACATG 
GTACCTGGGA GCCCTCAGGC ACGACACAGG ACAGTGGGTA CCAACACTCC ACCTAGCCCT 
GGTTTTGGCC GGCGGGCCGT CAACCCCACC ATGGCTGCCC CTGGTAGTCC CAGTTTGAGC 
CATCGGCAGG TGATGGGTCC ATCTGGTCCA GGTTTCCATG GTAACGTGGT TTCTGGCCAC 
CCAGCCAGTG CAGCTACCAC TCCTGGAAGC CCCAGCCTGG GCCGGCACCC AGTGGGAAGC 
CATCAAGTTC CAGGCCTCCA CAGCAGTGTG GTCACCACTC CGGGAAGCCC AAGCCTGGGC 
CGACACCCTG GTGCCCACCA AGGGAATCTG GCCTCCAGTC TCCACAGCAA TGCAGTCATC 
AGCCCTGGGA GCCCAAGCTT GGGCCGGCAC CTGGGTGGGT CTGGATCCGT GGTTCCTGGA 
AGCCCCAGCT TGGACCGGCA TGCAGCTTAT GGTGGCTACT CTACCCCTGA GGACCGAAGA 
CCAACACTGT CCCGGCAAAG CAGTGCCTCT GGCTACCAGG CCCCATCCAC ACCGTCATTC 
CCTGTCTCTC CTGCCTACTA CCCTGGCCTG AGCAGCCCTG CCACCTCACC CTCGCCAGAC 
TCAGCAGCCT TCCGGCAGGG GAGCCCCACA CCAGCCTTAC CAGAGAAGCG AAGAATGTCA 
GTGGGAGACC GGGCAGGCAG CCTCCCCAAC TATGCCACCA TCAATGGGAA AGTGTCTTCC 
TCGCCTGTCG CCAATGGCAT GGCCAGTGGG AGCAGCACCG TCTCCTTCTC TCACACGCTT 
CCGGACTTTT CTAAGTATTC GATGCCAGAC AATAGTCCGG AGACTCGGGC TAAAGTGAAG 
TTTGTCCAGG ACACTTCCAA GTATTGGTAC AAGCCTGAGA TCTCCAGAGA ACAGGCCATC 
GCACTCCTAA AGGACCAGGA GCCAGGAGCC TTCATCATCC GGGACAGCCA CTCCTTCCGA 
GGGGCTTATG GGCTGGCCAT GAAGGTGTCC TCACCCCCTC CAACCATCAC TCAGCAGGGC 
AAGAAAGGAG ACATGACCCA TGAGCTGGTG AGGCACTTTC TGATCGAGAC AGGCCCTAGA 
GGAGTGAAGC TCAAGGGTTG TCCCAATGAG CCAAACTTTG GTTCCCTTTC TGCCCTGGTC 
TATCAGCACT CCGTCATCCC ACTGGCCCTG CCCTGTAAAC TGGTCATTCC AAGCCGAGAC 
CCCACAGATG AATCAAAAGA TAGCTCAGGC CCTGCCAACT CAACCACTGA CCTGCTGAAG 
CAAGGAGCAG CTTGCAATGT GCTCTTCGTC AACTCTGTGG ATATGGAGTC ACTCACCGGG 
CCACAGGCCA TTTCCAAAGC CACATCTGAG ACTCTGGCTG CCGACCCCAC ACCGGCTGCC 
ACCATTGTTC ACTTCAAGGT CTCTGCCCAA GGAATCACAC TGACTGACAA TCAAAGAAAG 
CTCTTCTTTA GAAGACACTA CCCCCTCAAC ACTGTCACCT TCTGTGACCT GGATCCACAA 
GAAAGAAAGT GGATGAAGAC AGAGGGAGGC GCCCCTGCTA AGCTCTTTGG CTTCGTGGCC 
CGGAAGCAGG GCAGCACCAC AGACAACGCT TGCCACCTCT TCGCAGAGCT TGACCCCAAC 
CAGCCTGCGT CTGCCATCGT CAACTTCGTC TCCAAGGTCA TGCTGAGTGC TGGCCAGAAG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_017319858.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175347.1 Mus musculus tensin 1 (Tns1), transcript variant X8, mRNA.

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CloneID	OMu77076
Clone ID Related Accession (Same CDS sequence)	XM_017319858.1
Accession Version	XM_017319858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGGGGTT 
CAGGCTCGGG AGAGGCAGCC TGCAGAGCCC CCAGGCCCAC TCCGGAGGCG GGCAGCCAGT 
GATGGACAGT ATGAAAACCA GTCTCCGGAG GCCACATCCC CCCGTAGTCC TGGGGTCCGC 
TCCCCCGTCC AGTGCGTCTC CCCTGAGCTG GCTCTTACCA TTGCCCTCAA TCCTGGAGGG 
CGCCCCAAAG AGCCTCATTT GCATAGCTAC AAGGAGGCCT TTGAGGAGAT GGAGGGGACC 
TCCCCAAGCA GCCCGCCACA CAGTGTGGCG CGCTCTCCTC CAGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCCCACAAC CCAGCAGACA TCTTGCTGCA CCCCACAGGT 
GTTGCCAGAA GACTGATCCA GCCAGAGGAA GACGAGGGGG AGGAGGTGAC TAAGCCTCCA 
GAAGAGCCCC GGAGCTATGT GGAGTCCGTG GCGAGGACAG CGGTGGCAGG GCCTCGAGCT 
CAGGACGTTG AGCCTAAGAG CTTCAGCGCA CCAGCTGCTC ACGCCTATGG GCACGAGACA 
CCCCTGAGGA ACGGGACCCC AGGGGGCTCC TTTGTCTCCC CGAGCCCCCT CTCCACAAGC 
AGCCCCATCC TCAGTGCTGA CAGCACTTCC GTGGGCAGTT TCCCATCAGT GGTGAGCAGT 
GACCAGGGTC CCCGGACACC CTTCCAGCCT ATGCTGGACT CCAGCATCCG CTCAGGCAGC 
TTGGGGCAGC CGAGCCCTGC AGCACTGAGT TATCAGAGCT CCTCGCCTGT CCCTGTTGGT 
GGCAGCAGCT ACAACAGCCC TGACTACTCC CTCCAACCAT TCAGCTCTTC TCCAGAAAGC 
CAAGGCCAGC CACAGTACAG TGCGGCCAGT GTCCACATGG TACCTGGGAG CCCTCAGGCA 
CGACACAGGA CAGTGGGTAC CAACACTCCA CCTAGCCCTG GTTTTGGCCG GCGGGCCGTC 
AACCCCACCA TGGCTGCCCC TGGTAGTCCC AGTTTGAGCC ATCGGCAGGT GATGGGTCCA 
TCTGGTCCAG GTTTCCATGG TAACGTGGTT TCTGGCCACC CAGCCAGTGC AGCTACCACT 
CCTGGAAGCC CCAGCCTGGG CCGGCACCCA GTGGGAAGCC ATCAAGTTCC AGGCCTCCAC 
AGCAGTGTGG TCACCACTCC GGGAAGCCCA AGCCTGGGCC GACACCCTGG TGCCCACCAA 
GGGAATCTGG CCTCCAGTCT CCACAGCAAT GCAGTCATCA GCCCTGGGAG CCCAAGCTTG 
GGCCGGCACC TGGGTGGGTC TGGATCCGTG GTTCCTGGAA GCCCCAGCTT GGACCGGCAT 
GCAGCTTATG GTGGCTACTC TACCCCTGAG GACCGAAGAC CAACACTGTC CCGGCAAAGC 
AGTGCCTCTG GCTACCAGGC CCCATCCACA CCGTCATTCC CTGTCTCTCC TGCCTACTAC 
CCTGGCCTGA GCAGCCCTGC CACCTCACCC TCGCCAGACT CAGCAGCCTT CCGGCAGGGG 
AGCCCCACAC CAGCCTTACC AGAGAAGCGA AGAATGTCAG TGGGAGACCG GGCAGGCAGC 
CTCCCCAACT ATGCCACCAT CAATGGGAAA GTGTCTTCCT CGCCTGTCGC CAATGGCATG 
GCCAGTGGGA GCAGCACCGT CTCCTTCTCT CACACGCTTC CGGACTTTTC TAAGTATTCG 
ATGCCAGACA ATAGTCCGGA GACTCGGGCT AAAGTGAAGT TTGTCCAGGA CACTTCCAAG 
TATTGGTACA AGCCTGAGAT CTCCAGAGAA CAGGCCATCG CACTCCTAAA GGACCAGGAG 
CCAGGAGCCT TCATCATCCG GGACAGCCAC TCCTTCCGAG GGGCTTATGG GCTGGCCATG 
AAGGTGTCCT CACCCCCTCC AACCATCACT CAGCAGGGCA AGAAAGGAGA CATGACCCAT 
GAGCTGGTGA GGCACTTTCT GATCGAGACA GGCCCTAGAG GAGTGAAGCT CAAGGGTTGT 
CCCAATGAGC CAAACTTTGG TTCCCTTTCT GCCCTGGTCT ATCAGCACTC CGTCATCCCA 
CTGGCCCTGC CCTGTAAACT GGTCATTCCA AGCCGAGACC CCACAGATGA ATCAAAAGAT 
AGCTCAGGCC CTGCCAACTC AACCACTGAC CTGCTGAAGC AAGGAGCAGC TTGCAATGTG 
CTCTTCGTCA ACTCTGTGGA TATGGAGTCA CTCACCGGGC CACAGGCCAT TTCCAAAGCC 
ACATCTGAGA CTCTGGCTGC CGACCCCACA CCGGCTGCCA CCATTGTTCA CTTCAAGGTC 
TCTGCCCAAG GAATCACACT GACTGACAAT CAAAGAAAGC TCTTCTTTAG AAGACACTAC 
CCCCTCAACA CTGTCACCTT CTGTGACCTG GATCCACAAG AAAGAAAGTG GATGAAGACA 
GAGGGAGGCG CCCCTGCTAA GCTCTTTGGC TTCGTGGCCC GGAAGCAGGG CAGCACCACA 
GACAACGCTT GCCACCTCTT CGCAGAGCTT GACCCCAACC AGCCTGCGTC TGCCATCGTC 
AACTTCGTCT CCAAGGTCAT GCTGAGTGCT GGCCAGAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175347.1
CDS	16..5700
Translation	MLGDWGKGRGQPTGSPCPPWEHILFWNLGVSLPPCCSRESGVPWTQGALRRKLLVLPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_017319858.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319858.1

ORF Insert Sequence:

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGGGGTT 
CAGGCTCGGG AGAGGCAGCC TGCAGAGCCC CCAGGCCCAC TCCGGAGGCG GGCAGCCAGT 
GATGGACAGT ATGAAAACCA GTCTCCGGAG GCCACATCCC CCCGTAGTCC TGGGGTCCGC 
TCCCCCGTCC AGTGCGTCTC CCCTGAGCTG GCTCTTACCA TTGCCCTCAA TCCTGGAGGG 
CGCCCCAAAG AGCCTCATTT GCATAGCTAC AAGGAGGCCT TTGAGGAGAT GGAGGGGACC 
TCCCCAAGCA GCCCGCCACA CAGTGTGGCG CGCTCTCCTC CAGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCCCACAAC CCAGCAGACA TCTTGCTGCA CCCCACAGGT 
GTTGCCAGAA GACTGATCCA GCCAGAGGAA GACGAGGGGG AGGAGGTGAC TAAGCCTCCA 
GAAGAGCCCC GGAGCTATGT GGAGTCCGTG GCGAGGACAG CGGTGGCAGG GCCTCGAGCT 
CAGGACGTTG AGCCTAAGAG CTTCAGCGCA CCAGCTGCTC ACGCCTATGG GCACGAGACA 
CCCCTGAGGA ACGGGACCCC AGGGGGCTCC TTTGTCTCCC CGAGCCCCCT CTCCACAAGC 
AGCCCCATCC TCAGTGCTGA CAGCACTTCC GTGGGCAGTT TCCCATCAGT GGTGAGCAGT 
GACCAGGGTC CCCGGACACC CTTCCAGCCT ATGCTGGACT CCAGCATCCG CTCAGGCAGC 
TTGGGGCAGC CGAGCCCTGC AGCACTGAGT TATCAGAGCT CCTCGCCTGT CCCTGTTGGT 
GGCAGCAGCT ACAACAGCCC TGACTACTCC CTCCAACCAT TCAGCTCTTC TCCAGAAAGC 
CAAGGCCAGC CACAGTACAG TGCGGCCAGT GTCCACATGG TACCTGGGAG CCCTCAGGCA 
CGACACAGGA CAGTGGGTAC CAACACTCCA CCTAGCCCTG GTTTTGGCCG GCGGGCCGTC 
AACCCCACCA TGGCTGCCCC TGGTAGTCCC AGTTTGAGCC ATCGGCAGGT GATGGGTCCA 
TCTGGTCCAG GTTTCCATGG TAACGTGGTT TCTGGCCACC CAGCCAGTGC AGCTACCACT 
CCTGGAAGCC CCAGCCTGGG CCGGCACCCA GTGGGAAGCC ATCAAGTTCC AGGCCTCCAC 
AGCAGTGTGG TCACCACTCC GGGAAGCCCA AGCCTGGGCC GACACCCTGG TGCCCACCAA 
GGGAATCTGG CCTCCAGTCT CCACAGCAAT GCAGTCATCA GCCCTGGGAG CCCAAGCTTG 
GGCCGGCACC TGGGTGGGTC TGGATCCGTG GTTCCTGGAA GCCCCAGCTT GGACCGGCAT 
GCAGCTTATG GTGGCTACTC TACCCCTGAG GACCGAAGAC CAACACTGTC CCGGCAAAGC 
AGTGCCTCTG GCTACCAGGC CCCATCCACA CCGTCATTCC CTGTCTCTCC TGCCTACTAC 
CCTGGCCTGA GCAGCCCTGC CACCTCACCC TCGCCAGACT CAGCAGCCTT CCGGCAGGGG 
AGCCCCACAC CAGCCTTACC AGAGAAGCGA AGAATGTCAG TGGGAGACCG GGCAGGCAGC 
CTCCCCAACT ATGCCACCAT CAATGGGAAA GTGTCTTCCT CGCCTGTCGC CAATGGCATG 
GCCAGTGGGA GCAGCACCGT CTCCTTCTCT CACACGCTTC CGGACTTTTC TAAGTATTCG 
ATGCCAGACA ATAGTCCGGA GACTCGGGCT AAAGTGAAGT TTGTCCAGGA CACTTCCAAG 
TATTGGTACA AGCCTGAGAT CTCCAGAGAA CAGGCCATCG CACTCCTAAA GGACCAGGAG 
CCAGGAGCCT TCATCATCCG GGACAGCCAC TCCTTCCGAG GGGCTTATGG GCTGGCCATG 
AAGGTGTCCT CACCCCCTCC AACCATCACT CAGCAGGGCA AGAAAGGAGA CATGACCCAT 
GAGCTGGTGA GGCACTTTCT GATCGAGACA GGCCCTAGAG GAGTGAAGCT CAAGGGTTGT 
CCCAATGAGC CAAACTTTGG TTCCCTTTCT GCCCTGGTCT ATCAGCACTC CGTCATCCCA 
CTGGCCCTGC CCTGTAAACT GGTCATTCCA AGCCGAGACC CCACAGATGA ATCAAAAGAT 
AGCTCAGGCC CTGCCAACTC AACCACTGAC CTGCTGAAGC AAGGAGCAGC TTGCAATGTG 
CTCTTCGTCA ACTCTGTGGA TATGGAGTCA CTCACCGGGC CACAGGCCAT TTCCAAAGCC 
ACATCTGAGA CTCTGGCTGC CGACCCCACA CCGGCTGCCA CCATTGTTCA CTTCAAGGTC 
TCTGCCCAAG GAATCACACT GACTGACAAT CAAAGAAAGC TCTTCTTTAG AAGACACTAC 
CCCCTCAACA CTGTCACCTT CTGTGACCTG GATCCACAAG AAAGAAAGTG GATGAAGACA 
GAGGGAGGCG CCCCTGCTAA GCTCTTTGGC TTCGTGGCCC GGAAGCAGGG CAGCACCACA 
GACAACGCTT GCCACCTCTT CGCAGAGCTT GACCCCAACC AGCCTGCGTC TGCCATCGTC 
AACTTCGTCT CCAAGGTCAT GCTGAGTGCT GGCCAGAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_017319859.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175348.1 Mus musculus tensin 1 (Tns1), transcript variant X9, mRNA.

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CloneID	OMu77077
Clone ID Related Accession (Same CDS sequence)	XM_017319859.1
Accession Version	XM_017319859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGA AGTGGTGGGA CACACCCAAG GGCCGCTGGA TGGGAGCCTG 
TACGCCAAGG TGAAGAAGAA GGATTCCCTG AATGGCAGTT CGGGGCCTGT CACTACTGCA 
CGGCCTGCCC TGTCAGCCAC TCCCAACCAC GTGGAACACA CCCTGTCTGT GAGCAGCGAC 
TCTGGCAACT CCACAGCCTC CACTAAGACA GACAAGACCG ATGAGCCAGT CTCTGGGGCT 
ACCACTGCCC CTGCTGCCCT GAGTCCTCAG GAGAAGAAAG AGCTGGACCG CCTACTAAGT 
GGCTTTGGTG TGGATAGAGA AAAGCAAGGC GCCATGTACC GGGCTCAGCA ACTCAGATCC 
CATCCAGGCG GGGGCCCGAC TGTGCCCTCC CCTGGCCGCC ACATTGTCCC AGCCCAGGTT 
CACGTCAATG GTGGGGCCCT GGCATCTGAG CGGGAGACAG ACATTCTAGA TGATGAGCTG 
CCCATCCAGG ACGGGCAGAG TGGGGGCAGC ATGGGAACGC TGTCCTCCCT GGATGGGGTC 
ACCAACACCA GTGAAAGTGG CTACCCAGAG ACCCTGTCCC CACTGACCAA TGGCCTGGAC 
AAACCCTATT CCACAGAACC TGTGCTCAAC GGCGGAGGCT ACCCCTATGA GGCTGCCAAC 
CGGGTGATTC CTGTCCACAG CAGTCACTCG GCCCCCATTC GACCCTCCTA CTCTGCTCAG 
GAGGGTTTAG CTGGCTACCA GCGTGAGGGA CCCCACCCAG CCTGGTCACA GCAAGTGACC 
AGTGCCCACT GTGGCTGTGA CCCCAGTGGT CTATTCAGAT CCCAGTCCTT TCCAGATGTG 
GAGCCCCAGC TGCCCCAAGC TCCAACCCGT GGGGGTAGTA GCCGAGAGGC TGTGCAGAGG 
GGTCTGAATT CATGGCAACA GCAGCAGCCC CACCCACCTC CCCGCCAGCA GGAGAGATCC 
CCACTGCAGA GTCTTGCCCG CAGCAAGCCT AGCCCCCAGC TGTCAGCAGA GACCCCCGTA 
GCTGCTCTCC CAGAGTTCCC AAGGGCAGCG TCCCAGCAGG AGATCGAGCA GTCCATCGAA 
ACCCTCAATA TGTTGATGCT GGACCTGGAG CCAGCCTCCG CAGCTGCCCC TCTTCATAAG 
TCCCAGAGTG TCCCTGGGGC CTGGCCAGGG GCTTCTCCAC TCTCTTCCCA GCCCCTCTTG 
GGGTCTTCCC GCCAGTCCCA CCCACTTACC CAGTCCCGAT CTGGCTATAT CCCCAGTGGG 
CATTCTCTGG GAACCCCGGA GCTGGTCTCT TCTGGCAGGC CTTATTCACC TTACGATTAT 
CAGCTGCATC CAGCTGGATC CAACCAGAGT TTCCATCCAA AAAGTCCAGC CTCTTCCACC 
TTCCTTCCAA GCCCTCATAG CTCTGCGGGG CCTCAGGAGC CCCCAGCCTC TCTCCCTGGT 
CTCATCGCTC AGCCCCAGCT CCCACCAAAG GAAACTACAT CAGATCCCTC CCGAACTCCA 
GAGGAAGAGC CGCTGAACCT GGAAGGGCTG GTAGCCCATC GGGTAGCAGG GGTTCAGGCT 
CGGGAGAGGC AGCCTGCAGA GCCCCCAGGC CCACTCCGGA GGCGGGCAGC CAGTGATGGA 
CAGTATGAAA ACCAGTCTCC GGAGGCCACA TCCCCCCGTA GTCCTGGGGT CCGCTCCCCC 
GTCCAGTGCG TCTCCCCTGA GCTGGCTCTT ACCATTGCCC TCAATCCTGG AGGGCGCCCC 
AAAGAGCCTC ATTTGCATAG CTACAAGGAG GCCTTTGAGG AGATGGAGGG GACCTCCCCA 
AGCAGCCCGC CACACAGTGT GGCGCGCTCT CCTCCAGGTC TGGCCAAGAC ACCCCTGTCT 
GCTCTGGGCC TGAAACCCCA CAACCCAGCA GACATCTTGC TGCACCCCAC AGGTGTTGCC 
AGAAGACTGA TCCAGCCAGA GGAAGACGAG GGGGAGGAGG TGACTAAGCC TCCAGAAGAG 
CCCCGGAGCT ATGTGGAGTC CGTGGCGAGG ACAGCGGTGG CAGGGCCTCG AGCTCAGGAC 
GTTGAGCCTA AGAGCTTCAG CGCACCAGCT GCTCACGCCT ATGGGCACGA GACACCCCTG 
AGGAACGGGA CCCCAGGGGG CTCCTTTGTC TCCCCGAGCC CCCTCTCCAC AAGCAGCCCC 
ATCCTCAGTG CTGACAGCAC TTCCGTGGGC AGTTTCCCAT CAGTGGTGAG CAGTGACCAG 
GGTCCCCGGA CACCCTTCCA GCCTATGCTG GACTCCAGCA TCCGCTCAGG CAGCTTGGGG 
CAGCCGAGCC CTGCAGCACT GAGTTATCAG AGCTCCTCGC CTGTCCCTGT TGGTGGCAGC 
AGCTACAACA GCCCTGACTA CTCCCTCCAA CCATTCAGCT CTTCTCCAGA AAGCCAAGGC 
CAGCCACAGT ACAGTGCGGC CAGTGTCCAC ATGGTACCTG GGAGCCCTCA GGCACGACAC 
AGGACAGTGG GTACCAACAC TCCACCTAGC CCTGGTTTTG GCCGGCGGGC CGTCAACCCC 
ACCATGGCTG CCCCTGGTAG TCCCAGTTTG AGCCATCGGC AGGTGATGGG TCCATCTGGT 
CCAGGTTTCC ATGGTAACGT GGTTTCTGGC CACCCAGCCA GTGCAGCTAC CACTCCTGGA 
AGCCCCAGCC TGGGCCGGCA CCCAGTGGGA AGCCATCAAG TTCCAGGCCT CCACAGCAGT 
GTGGTCACCA CTCCGGGAAG CCCAAGCCTG GGCCGACACC CTGGTGCCCA CCAAGGGAAT 
CTGGCCTCCA GTCTCCACAG CAATGCAGTC ATCAGCCCTG GGAGCCCAAG CTTGGGCCGG 
CACCTGGGTG GGTCTGGATC CGTGGTTCCT GGAAGCCCCA GCTTGGACCG GCATGCAGCT 
TATGGTGGCT ACTCTACCCC TGAGGACCGA AGACCAACAC TGTCCCGGCA AAGCAGTGCC 
TCTGGCTACC AGGCCCCATC CACACCGTCA TTCCCTGTCT CTCCTGCCTA CTACCCTGGC 
CTGAGCAGCC CTGCCACCTC ACCCTCGCCA GACTCAGCAG CCTTCCGGCA GGGGAGCCCC 
ACACCAGCCT TACCAGAGAA GCGAAGAATG TCAGTGGGAG ACCGGGCAGG CAGCCTCCCC 
AACTATGCCA CCATCAATGG GAAAGTGTCT TCCTCGCCTG TCGCCAATGG CATGGCCAGT 
GGGAGCAGCA CCGTCTCCTT CTCTCACACG CTTCCGGACT TTTCTAAGTA TTCGATGCCA 
GACAATAGTC CGGAGACTCG GGCTAAAGTG AAGTTTGTCC AGGACACTTC CAAGTATTGG 
TACAAGCCTG AGATCTCCAG AGAACAGGCC ATCGCACTCC TAAAGGACCA GGAGCCAGGA 
GCCTTCATCA TCCGGGACAG CCACTCCTTC CGAGGGGCTT ATGGGCTGGC CATGAAGGTG 
TCCTCACCCC CTCCAACCAT CACTCAGCAG GGCAAGAAAG GAGACATGAC CCATGAGCTG 
GTGAGGCACT TTCTGATCGA GACAGGCCCT AGAGGAGTGA AGCTCAAGGG TTGTCCCAAT 
GAGCCAAACT TTGGTTCCCT TTCTGCCCTG GTCTATCAGC ACTCCGTCAT CCCACTGGCC 
CTGCCCTGTA AACTGGTCAT TCCAAGCCGA GACCCCACAG ATGAATCAAA AGATAGCTCA 
GGCCCTGCCA ACTCAACCAC TGACCTGCTG AAGCAAGGAG CAGCTTGCAA TGTGCTCTTC 
GTCAACTCTG TGGATATGGA GTCACTCACC GGGCCACAGG CCATTTCCAA AGCCACATCT 
GAGACTCTGG CTGCCGACCC CACACCGGCT GCCACCATTG TTCACTTCAA GGTCTCTGCC 
CAAGGAATCA CACTGACTGA CAATCAAAGA AAGCTCTTCT TTAGAAGACA CTACCCCCTC 
AACACTGTCA CCTTCTGTGA CCTGGATCCA CAAGAAAGAA AGTGGATGAA GACAGAGGGA 
GGCGCCCCTG CTAAGCTCTT TGGCTTCGTG GCCCGGAAGC AGGGCAGCAC CACAGACAAC 
GCTTGCCACC TCTTCGCAGA GCTTGACCCC AACCAGCCTG CGTCTGCCAT CGTCAACTTC 
GTCTCCAAGG TCATGCTGAG TGCTGGCCAG AAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175348.1
CDS	16..5694
Translation	MLGDWGKGRGQPTGSPCPPWEHILFWNLGVSLPPCCSRESGVPWTQGALRRKLLVLPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEEVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_017319859.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319859.1

ORF Insert Sequence:

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGA AGTGGTGGGA CACACCCAAG GGCCGCTGGA TGGGAGCCTG 
TACGCCAAGG TGAAGAAGAA GGATTCCCTG AATGGCAGTT CGGGGCCTGT CACTACTGCA 
CGGCCTGCCC TGTCAGCCAC TCCCAACCAC GTGGAACACA CCCTGTCTGT GAGCAGCGAC 
TCTGGCAACT CCACAGCCTC CACTAAGACA GACAAGACCG ATGAGCCAGT CTCTGGGGCT 
ACCACTGCCC CTGCTGCCCT GAGTCCTCAG GAGAAGAAAG AGCTGGACCG CCTACTAAGT 
GGCTTTGGTG TGGATAGAGA AAAGCAAGGC GCCATGTACC GGGCTCAGCA ACTCAGATCC 
CATCCAGGCG GGGGCCCGAC TGTGCCCTCC CCTGGCCGCC ACATTGTCCC AGCCCAGGTT 
CACGTCAATG GTGGGGCCCT GGCATCTGAG CGGGAGACAG ACATTCTAGA TGATGAGCTG 
CCCATCCAGG ACGGGCAGAG TGGGGGCAGC ATGGGAACGC TGTCCTCCCT GGATGGGGTC 
ACCAACACCA GTGAAAGTGG CTACCCAGAG ACCCTGTCCC CACTGACCAA TGGCCTGGAC 
AAACCCTATT CCACAGAACC TGTGCTCAAC GGCGGAGGCT ACCCCTATGA GGCTGCCAAC 
CGGGTGATTC CTGTCCACAG CAGTCACTCG GCCCCCATTC GACCCTCCTA CTCTGCTCAG 
GAGGGTTTAG CTGGCTACCA GCGTGAGGGA CCCCACCCAG CCTGGTCACA GCAAGTGACC 
AGTGCCCACT GTGGCTGTGA CCCCAGTGGT CTATTCAGAT CCCAGTCCTT TCCAGATGTG 
GAGCCCCAGC TGCCCCAAGC TCCAACCCGT GGGGGTAGTA GCCGAGAGGC TGTGCAGAGG 
GGTCTGAATT CATGGCAACA GCAGCAGCCC CACCCACCTC CCCGCCAGCA GGAGAGATCC 
CCACTGCAGA GTCTTGCCCG CAGCAAGCCT AGCCCCCAGC TGTCAGCAGA GACCCCCGTA 
GCTGCTCTCC CAGAGTTCCC AAGGGCAGCG TCCCAGCAGG AGATCGAGCA GTCCATCGAA 
ACCCTCAATA TGTTGATGCT GGACCTGGAG CCAGCCTCCG CAGCTGCCCC TCTTCATAAG 
TCCCAGAGTG TCCCTGGGGC CTGGCCAGGG GCTTCTCCAC TCTCTTCCCA GCCCCTCTTG 
GGGTCTTCCC GCCAGTCCCA CCCACTTACC CAGTCCCGAT CTGGCTATAT CCCCAGTGGG 
CATTCTCTGG GAACCCCGGA GCTGGTCTCT TCTGGCAGGC CTTATTCACC TTACGATTAT 
CAGCTGCATC CAGCTGGATC CAACCAGAGT TTCCATCCAA AAAGTCCAGC CTCTTCCACC 
TTCCTTCCAA GCCCTCATAG CTCTGCGGGG CCTCAGGAGC CCCCAGCCTC TCTCCCTGGT 
CTCATCGCTC AGCCCCAGCT CCCACCAAAG GAAACTACAT CAGATCCCTC CCGAACTCCA 
GAGGAAGAGC CGCTGAACCT GGAAGGGCTG GTAGCCCATC GGGTAGCAGG GGTTCAGGCT 
CGGGAGAGGC AGCCTGCAGA GCCCCCAGGC CCACTCCGGA GGCGGGCAGC CAGTGATGGA 
CAGTATGAAA ACCAGTCTCC GGAGGCCACA TCCCCCCGTA GTCCTGGGGT CCGCTCCCCC 
GTCCAGTGCG TCTCCCCTGA GCTGGCTCTT ACCATTGCCC TCAATCCTGG AGGGCGCCCC 
AAAGAGCCTC ATTTGCATAG CTACAAGGAG GCCTTTGAGG AGATGGAGGG GACCTCCCCA 
AGCAGCCCGC CACACAGTGT GGCGCGCTCT CCTCCAGGTC TGGCCAAGAC ACCCCTGTCT 
GCTCTGGGCC TGAAACCCCA CAACCCAGCA GACATCTTGC TGCACCCCAC AGGTGTTGCC 
AGAAGACTGA TCCAGCCAGA GGAAGACGAG GGGGAGGAGG TGACTAAGCC TCCAGAAGAG 
CCCCGGAGCT ATGTGGAGTC CGTGGCGAGG ACAGCGGTGG CAGGGCCTCG AGCTCAGGAC 
GTTGAGCCTA AGAGCTTCAG CGCACCAGCT GCTCACGCCT ATGGGCACGA GACACCCCTG 
AGGAACGGGA CCCCAGGGGG CTCCTTTGTC TCCCCGAGCC CCCTCTCCAC AAGCAGCCCC 
ATCCTCAGTG CTGACAGCAC TTCCGTGGGC AGTTTCCCAT CAGTGGTGAG CAGTGACCAG 
GGTCCCCGGA CACCCTTCCA GCCTATGCTG GACTCCAGCA TCCGCTCAGG CAGCTTGGGG 
CAGCCGAGCC CTGCAGCACT GAGTTATCAG AGCTCCTCGC CTGTCCCTGT TGGTGGCAGC 
AGCTACAACA GCCCTGACTA CTCCCTCCAA CCATTCAGCT CTTCTCCAGA AAGCCAAGGC 
CAGCCACAGT ACAGTGCGGC CAGTGTCCAC ATGGTACCTG GGAGCCCTCA GGCACGACAC 
AGGACAGTGG GTACCAACAC TCCACCTAGC CCTGGTTTTG GCCGGCGGGC CGTCAACCCC 
ACCATGGCTG CCCCTGGTAG TCCCAGTTTG AGCCATCGGC AGGTGATGGG TCCATCTGGT 
CCAGGTTTCC ATGGTAACGT GGTTTCTGGC CACCCAGCCA GTGCAGCTAC CACTCCTGGA 
AGCCCCAGCC TGGGCCGGCA CCCAGTGGGA AGCCATCAAG TTCCAGGCCT CCACAGCAGT 
GTGGTCACCA CTCCGGGAAG CCCAAGCCTG GGCCGACACC CTGGTGCCCA CCAAGGGAAT 
CTGGCCTCCA GTCTCCACAG CAATGCAGTC ATCAGCCCTG GGAGCCCAAG CTTGGGCCGG 
CACCTGGGTG GGTCTGGATC CGTGGTTCCT GGAAGCCCCA GCTTGGACCG GCATGCAGCT 
TATGGTGGCT ACTCTACCCC TGAGGACCGA AGACCAACAC TGTCCCGGCA AAGCAGTGCC 
TCTGGCTACC AGGCCCCATC CACACCGTCA TTCCCTGTCT CTCCTGCCTA CTACCCTGGC 
CTGAGCAGCC CTGCCACCTC ACCCTCGCCA GACTCAGCAG CCTTCCGGCA GGGGAGCCCC 
ACACCAGCCT TACCAGAGAA GCGAAGAATG TCAGTGGGAG ACCGGGCAGG CAGCCTCCCC 
AACTATGCCA CCATCAATGG GAAAGTGTCT TCCTCGCCTG TCGCCAATGG CATGGCCAGT 
GGGAGCAGCA CCGTCTCCTT CTCTCACACG CTTCCGGACT TTTCTAAGTA TTCGATGCCA 
GACAATAGTC CGGAGACTCG GGCTAAAGTG AAGTTTGTCC AGGACACTTC CAAGTATTGG 
TACAAGCCTG AGATCTCCAG AGAACAGGCC ATCGCACTCC TAAAGGACCA GGAGCCAGGA 
GCCTTCATCA TCCGGGACAG CCACTCCTTC CGAGGGGCTT ATGGGCTGGC CATGAAGGTG 
TCCTCACCCC CTCCAACCAT CACTCAGCAG GGCAAGAAAG GAGACATGAC CCATGAGCTG 
GTGAGGCACT TTCTGATCGA GACAGGCCCT AGAGGAGTGA AGCTCAAGGG TTGTCCCAAT 
GAGCCAAACT TTGGTTCCCT TTCTGCCCTG GTCTATCAGC ACTCCGTCAT CCCACTGGCC 
CTGCCCTGTA AACTGGTCAT TCCAAGCCGA GACCCCACAG ATGAATCAAA AGATAGCTCA 
GGCCCTGCCA ACTCAACCAC TGACCTGCTG AAGCAAGGAG CAGCTTGCAA TGTGCTCTTC 
GTCAACTCTG TGGATATGGA GTCACTCACC GGGCCACAGG CCATTTCCAA AGCCACATCT 
GAGACTCTGG CTGCCGACCC CACACCGGCT GCCACCATTG TTCACTTCAA GGTCTCTGCC 
CAAGGAATCA CACTGACTGA CAATCAAAGA AAGCTCTTCT TTAGAAGACA CTACCCCCTC 
AACACTGTCA CCTTCTGTGA CCTGGATCCA CAAGAAAGAA AGTGGATGAA GACAGAGGGA 
GGCGCCCCTG CTAAGCTCTT TGGCTTCGTG GCCCGGAAGC AGGGCAGCAC CACAGACAAC 
GCTTGCCACC TCTTCGCAGA GCTTGACCCC AACCAGCCTG CGTCTGCCAT CGTCAACTTC 
GTCTCCAAGG TCATGCTGAG TGCTGGCCAG AAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_017319860.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175349.1 Mus musculus tensin 1 (Tns1), transcript variant X10, mRNA.

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CloneID	OMu77078
Clone ID Related Accession (Same CDS sequence)	XM_017319860.1
Accession Version	XM_017319860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGA AGTGGTGGGA CACACCCAAG GGCCGCTGGA TGGGAGCCTG 
TACGCCAAGG TGAAGAAGAA GGATTCCCTG AATGGCAGTT CGGGGCCTGT CACTACTGCA 
CGGCCTGCCC TGTCAGCCAC TCCCAACCAC GTGGAACACA CCCTGTCTGT GAGCAGCGAC 
TCTGGCAACT CCACAGCCTC CACTAAGACA GACAAGACCG ATGAGCCAGT CTCTGGGGCT 
ACCACTGCCC CTGCTGCCCT GAGTCCTCAG GAGAAGAAAG AGCTGGACCG CCTACTAAGT 
GGCTTTGGTG TGGATAGAGA AAAGCAAGGC GCCATGTACC GGGCTCAGCA ACTCAGATCC 
CATCCAGGCG GGGGCCCGAC TGTGCCCTCC CCTGGCCGCC ACATTGTCCC AGCCCAGGTT 
CACGTCAATG GTGGGGCCCT GGCATCTGAG CGGGAGACAG ACATTCTAGA TGATGAGCTG 
CCCATCCAGG ACGGGCAGAG TGGGGGCAGC ATGGGAACGC TGTCCTCCCT GGATGGGGTC 
ACCAACACCA GTGAAAGTGG CTACCCAGAG ACCCTGTCCC CACTGACCAA TGGCCTGGAC 
AAACCCTATT CCACAGAACC TGTGCTCAAC GGCGGAGGCT ACCCCTATGA GGCTGCCAAC 
CGGGTGATTC CTGTCCACAG CAGTCACTCG GCCCCCATTC GACCCTCCTA CTCTGCTCAG 
GAGGGTTTAG CTGGCTACCA GCGTGAGGGA CCCCACCCAG CCTGGTCACA GCAAGTGACC 
AGTGCCCACT GTGGCTGTGA CCCCAGTGGT CTATTCAGAT CCCAGTCCTT TCCAGATGTG 
GAGCCCCAGC TGCCCCAAGC TCCAACCCGT GGGGGTAGTA GCCGAGAGGC TGTGCAGAGG 
GGTCTGAATT CATGGCAACA GCAGCAGCCC CACCCACCTC CCCGCCAGCA GGAGAGATCC 
CCACTGCAGA GTCTTGCCCG CAGCAAGCCT AGCCCCCAGC TGTCAGCAGA GACCCCCGTA 
GCTGCTCTCC CAGAGTTCCC AAGGGCAGCG TCCCAGCAGG AGATCGAGCA GTCCATCGAA 
ACCCTCAATA TGTTGATGCT GGACCTGGAG CCAGCCTCCG CAGCTGCCCC TCTTCATAAG 
TCCCAGAGTG TCCCTGGGGC CTGGCCAGGG GCTTCTCCAC TCTCTTCCCA GCCCCTCTTG 
GGGTCTTCCC GCCAGTCCCA CCCACTTACC CAGTCCCGAT CTGGCTATAT CCCCAGTGGG 
CATTCTCTGG GAACCCCGGA GCTGGTCTCT TCTGGCAGGC CTTATTCACC TTACGATTAT 
CAGCTGCATC CAGCTGGATC CAACCAGAGT TTCCATCCAA AAAGTCCAGC CTCTTCCACC 
TTCCTTCCAA GCCCTCATAG CTCTGCGGGG CCTCAGGAGC CCCCAGCCTC TCTCCCTGGT 
CTCATCGCTC AGCCCCAGCT CCCACCAAAG GAAACTACAT CAGATCCCTC CCGAACTCCA 
GAGGAAGAGC CGCTGAACCT GGAAGGGCTG GTAGCCCATC GGGTAGCAGG GGTTCAGGCT 
CGGGAGAGGC AGCCTGCAGA GCCCCCAGGC CCACTCCGGA GGCGGGCAGC CAGTGATGGA 
CAGTATGAAA ACCAGTCTCC GGAGGCCACA TCCCCCCGTA GTCCTGGGGT CCGCTCCCCC 
GTCCAGTGCG TCTCCCCTGA GCTGGCTCTT ACCATTGCCC TCAATCCTGG AGGGCGCCCC 
AAAGAGCCTC ATTTGCATAG CTACAAGGAG GCCTTTGAGG AGATGGAGGG GACCTCCCCA 
AGCAGCCCGC CACACAGTGT GGCGCGCTCT CCTCCAGGTC TGGCCAAGAC ACCCCTGTCT 
GCTCTGGGCC TGAAACCCCA CAACCCAGCA GACATCTTGC TGCACCCCAC AGGTGTTGCC 
AGAAGACTGA TCCAGCCAGA GCCCCGGAGC TATGTGGAGT CCGTGGCGAG GACAGCGGTG 
GCAGGGCCTC GAGCTCAGGA CGTTGAGCCT AAGAGCTTCA GCGCACCAGC TGCTCACGCC 
TATGGGCACG AGACACCCCT GAGGAACGGG ACCCCAGGGG GCTCCTTTGT CTCCCCGAGC 
CCCCTCTCCA CAAGCAGCCC CATCCTCAGT GCTGACAGCA CTTCCGTGGG CAGTTTCCCA 
TCAGTGGTGA GCAGTGACCA GGGTCCCCGG ACACCCTTCC AGCCTATGCT GGACTCCAGC 
ATCCGCTCAG GCAGCTTGGG GCAGCCGAGC CCTGCAGCAC TGAGTTATCA GAGCTCCTCG 
CCTGTCCCTG TTGGTGGCAG CAGCTACAAC AGCCCTGACT ACTCCCTCCA ACCATTCAGC 
TCTTCTCCAG AAAGCCAAGG CCAGCCACAG TACAGTGCGG CCAGTGTCCA CATGGTACCT 
GGGAGCCCTC AGGCACGACA CAGGACAGTG GGTACCAACA CTCCACCTAG CCCTGGTTTT 
GGCCGGCGGG CCGTCAACCC CACCATGGCT GCCCCTGGTA GTCCCAGTTT GAGCCATCGG 
CAGGTGATGG GTCCATCTGG TCCAGGTTTC CATGGTAACG TGGTTTCTGG CCACCCAGCC 
AGTGCAGCTA CCACTCCTGG AAGCCCCAGC CTGGGCCGGC ACCCAGTGGG AAGCCATCAA 
GTTCCAGGCC TCCACAGCAG TGTGGTCACC ACTCCGGGAA GCCCAAGCCT GGGCCGACAC 
CCTGGTGCCC ACCAAGGGAA TCTGGCCTCC AGTCTCCACA GCAATGCAGT CATCAGCCCT 
GGGAGCCCAA GCTTGGGCCG GCACCTGGGT GGGTCTGGAT CCGTGGTTCC TGGAAGCCCC 
AGCTTGGACC GGCATGCAGC TTATGGTGGC TACTCTACCC CTGAGGACCG AAGACCAACA 
CTGTCCCGGC AAAGCAGTGC CTCTGGCTAC CAGGCCCCAT CCACACCGTC ATTCCCTGTC 
TCTCCTGCCT ACTACCCTGG CCTGAGCAGC CCTGCCACCT CACCCTCGCC AGACTCAGCA 
GCCTTCCGGC AGGGGAGCCC CACACCAGCC TTACCAGAGA AGCGAAGAAT GTCAGTGGGA 
GACCGGGCAG GCAGCCTCCC CAACTATGCC ACCATCAATG GGAAAGTGTC TTCCTCGCCT 
GTCGCCAATG GCATGGCCAG TGGGAGCAGC ACCGTCTCCT TCTCTCACAC GCTTCCGGAC 
TTTTCTAAGT ATTCGATGCC AGACAATAGT CCGGAGACTC GGGCTAAAGT GAAGTTTGTC 
CAGGACACTT CCAAGTATTG GTACAAGCCT GAGATCTCCA GAGAACAGGC CATCGCACTC 
CTAAAGGACC AGGAGCCAGG AGCCTTCATC ATCCGGGACA GCCACTCCTT CCGAGGGGCT 
TATGGGCTGG CCATGAAGGT GTCCTCACCC CCTCCAACCA TCACTCAGCA GGGCAAGAAA 
GGAGACATGA CCCATGAGCT GGTGAGGCAC TTTCTGATCG AGACAGGCCC TAGAGGAGTG 
AAGCTCAAGG GTTGTCCCAA TGAGCCAAAC TTTGGTTCCC TTTCTGCCCT GGTCTATCAG 
CACTCCGTCA TCCCACTGGC CCTGCCCTGT AAACTGGTCA TTCCAAGCCG AGACCCCACA 
GATGAATCAA AAGATAGCTC AGGCCCTGCC AACTCAACCA CTGACCTGCT GAAGCAAGGA 
GCAGCTTGCA ATGTGCTCTT CGTCAACTCT GTGGATATGG AGTCACTCAC CGGGCCACAG 
GCCATTTCCA AAGCCACATC TGAGACTCTG GCTGCCGACC CCACACCGGC TGCCACCATT 
GTTCACTTCA AGGTCTCTGC CCAAGGAATC ACACTGACTG ACAATCAAAG AAAGCTCTTC 
TTTAGAAGAC ACTACCCCCT CAACACTGTC ACCTTCTGTG ACCTGGATCC ACAAGAAAGA 
AAGTGGATGA AGACAGAGGG AGGCGCCCCT GCTAAGCTCT TTGGCTTCGT GGCCCGGAAG 
CAGGGCAGCA CCACAGACAA CGCTTGCCAC CTCTTCGCAG AGCTTGACCC CAACCAGCCT 
GCGTCTGCCA TCGTCAACTT CGTCTCCAAG GTCATGCTGA GTGCTGGCCA GAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175349.1
CDS	16..5655
Translation	MLGDWGKGRGQPTGSPCPPWEHILFWNLGVSLPPCCSRESGVPWTQGALRRKLLVLPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEEVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_017319860.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319860.1

ORF Insert Sequence:

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGA AGTGGTGGGA CACACCCAAG GGCCGCTGGA TGGGAGCCTG 
TACGCCAAGG TGAAGAAGAA GGATTCCCTG AATGGCAGTT CGGGGCCTGT CACTACTGCA 
CGGCCTGCCC TGTCAGCCAC TCCCAACCAC GTGGAACACA CCCTGTCTGT GAGCAGCGAC 
TCTGGCAACT CCACAGCCTC CACTAAGACA GACAAGACCG ATGAGCCAGT CTCTGGGGCT 
ACCACTGCCC CTGCTGCCCT GAGTCCTCAG GAGAAGAAAG AGCTGGACCG CCTACTAAGT 
GGCTTTGGTG TGGATAGAGA AAAGCAAGGC GCCATGTACC GGGCTCAGCA ACTCAGATCC 
CATCCAGGCG GGGGCCCGAC TGTGCCCTCC CCTGGCCGCC ACATTGTCCC AGCCCAGGTT 
CACGTCAATG GTGGGGCCCT GGCATCTGAG CGGGAGACAG ACATTCTAGA TGATGAGCTG 
CCCATCCAGG ACGGGCAGAG TGGGGGCAGC ATGGGAACGC TGTCCTCCCT GGATGGGGTC 
ACCAACACCA GTGAAAGTGG CTACCCAGAG ACCCTGTCCC CACTGACCAA TGGCCTGGAC 
AAACCCTATT CCACAGAACC TGTGCTCAAC GGCGGAGGCT ACCCCTATGA GGCTGCCAAC 
CGGGTGATTC CTGTCCACAG CAGTCACTCG GCCCCCATTC GACCCTCCTA CTCTGCTCAG 
GAGGGTTTAG CTGGCTACCA GCGTGAGGGA CCCCACCCAG CCTGGTCACA GCAAGTGACC 
AGTGCCCACT GTGGCTGTGA CCCCAGTGGT CTATTCAGAT CCCAGTCCTT TCCAGATGTG 
GAGCCCCAGC TGCCCCAAGC TCCAACCCGT GGGGGTAGTA GCCGAGAGGC TGTGCAGAGG 
GGTCTGAATT CATGGCAACA GCAGCAGCCC CACCCACCTC CCCGCCAGCA GGAGAGATCC 
CCACTGCAGA GTCTTGCCCG CAGCAAGCCT AGCCCCCAGC TGTCAGCAGA GACCCCCGTA 
GCTGCTCTCC CAGAGTTCCC AAGGGCAGCG TCCCAGCAGG AGATCGAGCA GTCCATCGAA 
ACCCTCAATA TGTTGATGCT GGACCTGGAG CCAGCCTCCG CAGCTGCCCC TCTTCATAAG 
TCCCAGAGTG TCCCTGGGGC CTGGCCAGGG GCTTCTCCAC TCTCTTCCCA GCCCCTCTTG 
GGGTCTTCCC GCCAGTCCCA CCCACTTACC CAGTCCCGAT CTGGCTATAT CCCCAGTGGG 
CATTCTCTGG GAACCCCGGA GCTGGTCTCT TCTGGCAGGC CTTATTCACC TTACGATTAT 
CAGCTGCATC CAGCTGGATC CAACCAGAGT TTCCATCCAA AAAGTCCAGC CTCTTCCACC 
TTCCTTCCAA GCCCTCATAG CTCTGCGGGG CCTCAGGAGC CCCCAGCCTC TCTCCCTGGT 
CTCATCGCTC AGCCCCAGCT CCCACCAAAG GAAACTACAT CAGATCCCTC CCGAACTCCA 
GAGGAAGAGC CGCTGAACCT GGAAGGGCTG GTAGCCCATC GGGTAGCAGG GGTTCAGGCT 
CGGGAGAGGC AGCCTGCAGA GCCCCCAGGC CCACTCCGGA GGCGGGCAGC CAGTGATGGA 
CAGTATGAAA ACCAGTCTCC GGAGGCCACA TCCCCCCGTA GTCCTGGGGT CCGCTCCCCC 
GTCCAGTGCG TCTCCCCTGA GCTGGCTCTT ACCATTGCCC TCAATCCTGG AGGGCGCCCC 
AAAGAGCCTC ATTTGCATAG CTACAAGGAG GCCTTTGAGG AGATGGAGGG GACCTCCCCA 
AGCAGCCCGC CACACAGTGT GGCGCGCTCT CCTCCAGGTC TGGCCAAGAC ACCCCTGTCT 
GCTCTGGGCC TGAAACCCCA CAACCCAGCA GACATCTTGC TGCACCCCAC AGGTGTTGCC 
AGAAGACTGA TCCAGCCAGA GCCCCGGAGC TATGTGGAGT CCGTGGCGAG GACAGCGGTG 
GCAGGGCCTC GAGCTCAGGA CGTTGAGCCT AAGAGCTTCA GCGCACCAGC TGCTCACGCC 
TATGGGCACG AGACACCCCT GAGGAACGGG ACCCCAGGGG GCTCCTTTGT CTCCCCGAGC 
CCCCTCTCCA CAAGCAGCCC CATCCTCAGT GCTGACAGCA CTTCCGTGGG CAGTTTCCCA 
TCAGTGGTGA GCAGTGACCA GGGTCCCCGG ACACCCTTCC AGCCTATGCT GGACTCCAGC 
ATCCGCTCAG GCAGCTTGGG GCAGCCGAGC CCTGCAGCAC TGAGTTATCA GAGCTCCTCG 
CCTGTCCCTG TTGGTGGCAG CAGCTACAAC AGCCCTGACT ACTCCCTCCA ACCATTCAGC 
TCTTCTCCAG AAAGCCAAGG CCAGCCACAG TACAGTGCGG CCAGTGTCCA CATGGTACCT 
GGGAGCCCTC AGGCACGACA CAGGACAGTG GGTACCAACA CTCCACCTAG CCCTGGTTTT 
GGCCGGCGGG CCGTCAACCC CACCATGGCT GCCCCTGGTA GTCCCAGTTT GAGCCATCGG 
CAGGTGATGG GTCCATCTGG TCCAGGTTTC CATGGTAACG TGGTTTCTGG CCACCCAGCC 
AGTGCAGCTA CCACTCCTGG AAGCCCCAGC CTGGGCCGGC ACCCAGTGGG AAGCCATCAA 
GTTCCAGGCC TCCACAGCAG TGTGGTCACC ACTCCGGGAA GCCCAAGCCT GGGCCGACAC 
CCTGGTGCCC ACCAAGGGAA TCTGGCCTCC AGTCTCCACA GCAATGCAGT CATCAGCCCT 
GGGAGCCCAA GCTTGGGCCG GCACCTGGGT GGGTCTGGAT CCGTGGTTCC TGGAAGCCCC 
AGCTTGGACC GGCATGCAGC TTATGGTGGC TACTCTACCC CTGAGGACCG AAGACCAACA 
CTGTCCCGGC AAAGCAGTGC CTCTGGCTAC CAGGCCCCAT CCACACCGTC ATTCCCTGTC 
TCTCCTGCCT ACTACCCTGG CCTGAGCAGC CCTGCCACCT CACCCTCGCC AGACTCAGCA 
GCCTTCCGGC AGGGGAGCCC CACACCAGCC TTACCAGAGA AGCGAAGAAT GTCAGTGGGA 
GACCGGGCAG GCAGCCTCCC CAACTATGCC ACCATCAATG GGAAAGTGTC TTCCTCGCCT 
GTCGCCAATG GCATGGCCAG TGGGAGCAGC ACCGTCTCCT TCTCTCACAC GCTTCCGGAC 
TTTTCTAAGT ATTCGATGCC AGACAATAGT CCGGAGACTC GGGCTAAAGT GAAGTTTGTC 
CAGGACACTT CCAAGTATTG GTACAAGCCT GAGATCTCCA GAGAACAGGC CATCGCACTC 
CTAAAGGACC AGGAGCCAGG AGCCTTCATC ATCCGGGACA GCCACTCCTT CCGAGGGGCT 
TATGGGCTGG CCATGAAGGT GTCCTCACCC CCTCCAACCA TCACTCAGCA GGGCAAGAAA 
GGAGACATGA CCCATGAGCT GGTGAGGCAC TTTCTGATCG AGACAGGCCC TAGAGGAGTG 
AAGCTCAAGG GTTGTCCCAA TGAGCCAAAC TTTGGTTCCC TTTCTGCCCT GGTCTATCAG 
CACTCCGTCA TCCCACTGGC CCTGCCCTGT AAACTGGTCA TTCCAAGCCG AGACCCCACA 
GATGAATCAA AAGATAGCTC AGGCCCTGCC AACTCAACCA CTGACCTGCT GAAGCAAGGA 
GCAGCTTGCA ATGTGCTCTT CGTCAACTCT GTGGATATGG AGTCACTCAC CGGGCCACAG 
GCCATTTCCA AAGCCACATC TGAGACTCTG GCTGCCGACC CCACACCGGC TGCCACCATT 
GTTCACTTCA AGGTCTCTGC CCAAGGAATC ACACTGACTG ACAATCAAAG AAAGCTCTTC 
TTTAGAAGAC ACTACCCCCT CAACACTGTC ACCTTCTGTG ACCTGGATCC ACAAGAAAGA 
AAGTGGATGA AGACAGAGGG AGGCGCCCCT GCTAAGCTCT TTGGCTTCGT GGCCCGGAAG 
CAGGGCAGCA CCACAGACAA CGCTTGCCAC CTCTTCGCAG AGCTTGACCC CAACCAGCCT 
GCGTCTGCCA TCGTCAACTT CGTCTCCAAG GTCATGCTGA GTGCTGGCCA GAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_017319862.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175351.1 Mus musculus tensin 1 (Tns1), transcript variant X11, mRNA.

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CloneID	OMu77079
Clone ID Related Accession (Same CDS sequence)	XM_017319862.2 , XM_017319862.1
Accession Version	XM_017319862.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319862.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCGAC TGAGCTGGTG CTTCTCCTGC GTGATCCGCT GGGGCAAGTA CCTCTTCTCT 
TGCCTCCTGC CCCTGCGCCT CTGCCTTCGC AGCCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGGTT TAAAAGTGGT GGGACACACC CAAGGGCCGC TGGATGGGAG CCTGTACGCC 
AAGGTGAAGA AGAAGGATTC CCTGAATGGC AGTTCGGGGC CTGTCACTAC TGCACGGCCT 
GCCCTGTCAG CCACTCCCAA CCACGTGGAA CACACCCTGT CTGTGAGCAG CGACTCTGGC 
AACTCCACAG CCTCCACTAA GACAGACAAG ACCGATGAGC CAGTCTCTGG GGCTACCACT 
GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG AAAGAGCTGG ACCGCCTACT AAGTGGCTTT 
GGTGTGGATA GAGAAAAGCA AGGCGCCATG TACCGGGCTC AGCAACTCAG ATCCCATCCA 
GGCGGGGGCC CGACTGTGCC CTCCCCTGGC CGCCACATTG TCCCAGCCCA GGTTCACGTC 
AATGGTGGGG CCCTGGCATC TGAGCGGGAG ACAGACATTC TAGATGATGA GCTGCCCATC 
CAGGACGGGC AGAGTGGGGG CAGCATGGGA ACGCTGTCCT CCCTGGATGG GGTCACCAAC 
ACCAGTGAAA GTGGCTACCC AGAGACCCTG TCCCCACTGA CCAATGGCCT GGACAAACCC 
TATTCCACAG AACCTGTGCT CAACGGCGGA GGCTACCCCT ATGAGGCTGC CAACCGGGTG 
ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC ATTCGACCCT CCTACTCTGC TCAGGAGGGT 
TTAGCTGGCT ACCAGCGTGA GGGACCCCAC CCAGCCTGGT CACAGCAAGT GACCAGTGCC 
CACTGTGGCT GTGACCCCAG TGGTCTATTC AGATCCCAGT CCTTTCCAGA TGTGGAGCCC 
CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG 
AATTCATGGC AACAGCAGCA GCCCCACCCA CCTCCCCGCC AGCAGGAGAG ATCCCCACTG 
CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC CAGCTGTCAG CAGAGACCCC CGTAGCTGCT 
CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG CAGGAGATCG AGCAGTCCAT CGAAACCCTC 
AATATGTTGA TGCTGGACCT GGAGCCAGCC TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG 
AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT CCACTCTCTT CCCAGCCCCT CTTGGGGTCT 
TCCCGCCAGT CCCACCCACT TACCCAGTCC CGATCTGGCT ATATCCCCAG TGGGCATTCT 
CTGGGAACCC CGGAGCTGGT CTCTTCTGGC AGGCCTTATT CACCTTACGA TTATCAGCTG 
CATCCAGCTG GATCCAACCA GAGTTTCCAT CCAAAAAGTC CAGCCTCTTC CACCTTCCTT 
CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG GAGCCCCCAG CCTCTCTCCC TGGTCTCATC 
GCTCAGCCCC AGCTCCCACC AAAGGAAACT ACATCAGATC CCTCCCGAAC TCCAGAGGAA 
GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC CATCGGGTAG CAGTGTACAA TGCCAGACTA 
CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC 
CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG 
CGGGCAGCCA GTGATGGACA GTATGAAAAC CAGTCTCCGG AGGCCACATC CCCCCGTAGT 
CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC TCCCCTGAGC TGGCTCTTAC CATTGCCCTC 
AATCCTGGAG GGCGCCCCAA AGAGCCTCAT TTGCATAGCT ACAAGGAGGC CTTTGAGGAG 
ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA CACAGTGTGG CGCGCTCTCC TCCAGGTCTG 
GCCAAGACAC CCCTGTCTGC TCTGGGCCTG AAACCCCACA ACCCAGCAGA CATCTTGCTG 
CACCCCACAG GTGTTGCCAG AAGACTGATC CAGCCAGAGG AAGACGAGGG GGAGGAGGTG 
ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA 
GGGCCTCGAG CTCAGGACGT TGAGCCTAAG AGCTTCAGCG CACCAGCTGC TCACGCCTAT 
GGGCACGAGA CACCCCTGAG GAACGGGACC CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC 
CTCTCCACAA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CCGTGGGCAG TTTCCCATCA 
GTGGTGAGCA GTGACCAGGG TCCCCGGACA CCCTTCCAGC CTATGCTGGA CTCCAGCATC 
CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT GCAGCACTGA GTTATCAGAG CTCCTCGCCT 
GTCCCTGTTG GTGGCAGCAG CTACAACAGC CCTGACTACT CCCTCCAACC ATTCAGCTCT 
TCTCCAGAAA GCCAAGGCCA GCCACAGTAC AGTGCGGCCA GTGTCCACAT GGTACCTGGG 
AGCCCTCAGG CACGACACAG GACAGTGGGT ACCAACACTC CACCTAGCCC TGGTTTTGGC 
CGGCGGGCCG TCAACCCCAC CATGGCTGCC CCTGGTAGTC CCAGTTTGAG CCATCGGCAG 
GTGATGGGTC CATCTGGTCC AGGTTTCCAT GGTAACGTGG TTTCTGGCCA CCCAGCCAGT 
GCAGCTACCA CTCCTGGAAG CCCCAGCCTG GGCCGGCACC CAGTGGGAAG CCATCAAGTT 
CCAGGCCTCC ACAGCAGTGT GGTCACCACT CCGGGAAGCC CAAGCCTGGG CCGACACCCT 
GGTGCCCACC AAGGGAATCT GGCCTCCAGT CTCCACAGCA ATGCAGTCAT CAGCCCTGGG 
AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG TCTGGATCCG TGGTTCCTGG AAGCCCCAGC 
TTGGACCGGC ATGCAGCTTA TGGTGGCTAC TCTACCCCTG AGGACCGAAG ACCAACACTG 
TCCCGGCAAA GCAGTGCCTC TGGCTACCAG GCCCCATCCA CACCGTCATT CCCTGTCTCT 
CCTGCCTACT ACCCTGGCCT GAGCAGCCCT GCCACCTCAC CCTCGCCAGA CTCAGCAGCC 
TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA CCAGAGAAGC GAAGAATGTC AGTGGGAGAC 
CGGGCAGGCA GCCTCCCCAA CTATGCCACC ATCAATGGGA AAGTGTCTTC CTCGCCTGTC 
GCCAATGGCA TGGCCAGTGG GAGCAGCACC GTCTCCTTCT CTCACACGCT TCCGGACTTT 
TCTAAGTATT CGATGCCAGA CAATAGTCCG GAGACTCGGG CTAAAGTGAA GTTTGTCCAG 
GACACTTCCA AGTATTGGTA CAAGCCTGAG ATCTCCAGAG AACAGGCCAT CGCACTCCTA 
AAGGACCAGG AGCCAGGAGC CTTCATCATC CGGGACAGCC ACTCCTTCCG AGGGGCTTAT 
GGGCTGGCCA TGAAGGTGTC CTCACCCCCT CCAACCATCA CTCAGCAGGG CAAGAAAGGA 
GACATGACCC ATGAGCTGGT GAGGCACTTT CTGATCGAGA CAGGCCCTAG AGGAGTGAAG 
CTCAAGGGTT GTCCCAATGA GCCAAACTTT GGTTCCCTTT CTGCCCTGGT CTATCAGCAC 
TCCGTCATCC CACTGGCCCT GCCCTGTAAA CTGGTCATTC CAAGCCGAGA CCCCACAGAT 
GAATCAAAAG ATAGCTCAGG CCCTGCCAAC TCAACCACTG ACCTGCTGAA GCAAGGAGCA 
GCTTGCAATG TGCTCTTCGT CAACTCTGTG GATATGGAGT CACTCACCGG GCCACAGGCC 
ATTTCCAAAG CCACATCTGA GACTCTGGCT GCCGACCCCA CACCGGCTGC CACCATTGTT 
CACTTCAAGG TCTCTGCCCA AGGAATCACA CTGACTGACA ATCAAAGAAA GCTCTTCTTT 
AGAAGACACT ACCCCCTCAA CACTGTCACC TTCTGTGACC TGGATCCACA AGAAAGAAAG 
TGGATGAAGA CAGAGGGAGG CGCCCCTGCT AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG 
GGCAGCACCA CAGACAACGC TTGCCACCTC TTCGCAGAGC TTGACCCCAA CCAGCCTGCG 
TCTGCCATCG TCAACTTCGT CTCCAAGGTC ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175351.1
CDS	189..5885
Translation	MTRLSWCFSCVIRWGKYLFSCLLPLRLCLRSQPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_017319862.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319862.2

ORF Insert Sequence:

ATGACTCGAC TGAGCTGGTG CTTCTCCTGC GTGATCCGCT GGGGCAAGTA CCTCTTCTCT 
TGCCTCCTGC CCCTGCGCCT CTGCCTTCGC AGCCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGGTT TAAAAGTGGT GGGACACACC CAAGGGCCGC TGGATGGGAG CCTGTACGCC 
AAGGTGAAGA AGAAGGATTC CCTGAATGGC AGTTCGGGGC CTGTCACTAC TGCACGGCCT 
GCCCTGTCAG CCACTCCCAA CCACGTGGAA CACACCCTGT CTGTGAGCAG CGACTCTGGC 
AACTCCACAG CCTCCACTAA GACAGACAAG ACCGATGAGC CAGTCTCTGG GGCTACCACT 
GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG AAAGAGCTGG ACCGCCTACT AAGTGGCTTT 
GGTGTGGATA GAGAAAAGCA AGGCGCCATG TACCGGGCTC AGCAACTCAG ATCCCATCCA 
GGCGGGGGCC CGACTGTGCC CTCCCCTGGC CGCCACATTG TCCCAGCCCA GGTTCACGTC 
AATGGTGGGG CCCTGGCATC TGAGCGGGAG ACAGACATTC TAGATGATGA GCTGCCCATC 
CAGGACGGGC AGAGTGGGGG CAGCATGGGA ACGCTGTCCT CCCTGGATGG GGTCACCAAC 
ACCAGTGAAA GTGGCTACCC AGAGACCCTG TCCCCACTGA CCAATGGCCT GGACAAACCC 
TATTCCACAG AACCTGTGCT CAACGGCGGA GGCTACCCCT ATGAGGCTGC CAACCGGGTG 
ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC ATTCGACCCT CCTACTCTGC TCAGGAGGGT 
TTAGCTGGCT ACCAGCGTGA GGGACCCCAC CCAGCCTGGT CACAGCAAGT GACCAGTGCC 
CACTGTGGCT GTGACCCCAG TGGTCTATTC AGATCCCAGT CCTTTCCAGA TGTGGAGCCC 
CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG 
AATTCATGGC AACAGCAGCA GCCCCACCCA CCTCCCCGCC AGCAGGAGAG ATCCCCACTG 
CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC CAGCTGTCAG CAGAGACCCC CGTAGCTGCT 
CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG CAGGAGATCG AGCAGTCCAT CGAAACCCTC 
AATATGTTGA TGCTGGACCT GGAGCCAGCC TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG 
AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT CCACTCTCTT CCCAGCCCCT CTTGGGGTCT 
TCCCGCCAGT CCCACCCACT TACCCAGTCC CGATCTGGCT ATATCCCCAG TGGGCATTCT 
CTGGGAACCC CGGAGCTGGT CTCTTCTGGC AGGCCTTATT CACCTTACGA TTATCAGCTG 
CATCCAGCTG GATCCAACCA GAGTTTCCAT CCAAAAAGTC CAGCCTCTTC CACCTTCCTT 
CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG GAGCCCCCAG CCTCTCTCCC TGGTCTCATC 
GCTCAGCCCC AGCTCCCACC AAAGGAAACT ACATCAGATC CCTCCCGAAC TCCAGAGGAA 
GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC CATCGGGTAG CAGTGTACAA TGCCAGACTA 
CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC 
CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG 
CGGGCAGCCA GTGATGGACA GTATGAAAAC CAGTCTCCGG AGGCCACATC CCCCCGTAGT 
CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC TCCCCTGAGC TGGCTCTTAC CATTGCCCTC 
AATCCTGGAG GGCGCCCCAA AGAGCCTCAT TTGCATAGCT ACAAGGAGGC CTTTGAGGAG 
ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA CACAGTGTGG CGCGCTCTCC TCCAGGTCTG 
GCCAAGACAC CCCTGTCTGC TCTGGGCCTG AAACCCCACA ACCCAGCAGA CATCTTGCTG 
CACCCCACAG GTGTTGCCAG AAGACTGATC CAGCCAGAGG AAGACGAGGG GGAGGAGGTG 
ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA 
GGGCCTCGAG CTCAGGACGT TGAGCCTAAG AGCTTCAGCG CACCAGCTGC TCACGCCTAT 
GGGCACGAGA CACCCCTGAG GAACGGGACC CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC 
CTCTCCACAA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CCGTGGGCAG TTTCCCATCA 
GTGGTGAGCA GTGACCAGGG TCCCCGGACA CCCTTCCAGC CTATGCTGGA CTCCAGCATC 
CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT GCAGCACTGA GTTATCAGAG CTCCTCGCCT 
GTCCCTGTTG GTGGCAGCAG CTACAACAGC CCTGACTACT CCCTCCAACC ATTCAGCTCT 
TCTCCAGAAA GCCAAGGCCA GCCACAGTAC AGTGCGGCCA GTGTCCACAT GGTACCTGGG 
AGCCCTCAGG CACGACACAG GACAGTGGGT ACCAACACTC CACCTAGCCC TGGTTTTGGC 
CGGCGGGCCG TCAACCCCAC CATGGCTGCC CCTGGTAGTC CCAGTTTGAG CCATCGGCAG 
GTGATGGGTC CATCTGGTCC AGGTTTCCAT GGTAACGTGG TTTCTGGCCA CCCAGCCAGT 
GCAGCTACCA CTCCTGGAAG CCCCAGCCTG GGCCGGCACC CAGTGGGAAG CCATCAAGTT 
CCAGGCCTCC ACAGCAGTGT GGTCACCACT CCGGGAAGCC CAAGCCTGGG CCGACACCCT 
GGTGCCCACC AAGGGAATCT GGCCTCCAGT CTCCACAGCA ATGCAGTCAT CAGCCCTGGG 
AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG TCTGGATCCG TGGTTCCTGG AAGCCCCAGC 
TTGGACCGGC ATGCAGCTTA TGGTGGCTAC TCTACCCCTG AGGACCGAAG ACCAACACTG 
TCCCGGCAAA GCAGTGCCTC TGGCTACCAG GCCCCATCCA CACCGTCATT CCCTGTCTCT 
CCTGCCTACT ACCCTGGCCT GAGCAGCCCT GCCACCTCAC CCTCGCCAGA CTCAGCAGCC 
TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA CCAGAGAAGC GAAGAATGTC AGTGGGAGAC 
CGGGCAGGCA GCCTCCCCAA CTATGCCACC ATCAATGGGA AAGTGTCTTC CTCGCCTGTC 
GCCAATGGCA TGGCCAGTGG GAGCAGCACC GTCTCCTTCT CTCACACGCT TCCGGACTTT 
TCTAAGTATT CGATGCCAGA CAATAGTCCG GAGACTCGGG CTAAAGTGAA GTTTGTCCAG 
GACACTTCCA AGTATTGGTA CAAGCCTGAG ATCTCCAGAG AACAGGCCAT CGCACTCCTA 
AAGGACCAGG AGCCAGGAGC CTTCATCATC CGGGACAGCC ACTCCTTCCG AGGGGCTTAT 
GGGCTGGCCA TGAAGGTGTC CTCACCCCCT CCAACCATCA CTCAGCAGGG CAAGAAAGGA 
GACATGACCC ATGAGCTGGT GAGGCACTTT CTGATCGAGA CAGGCCCTAG AGGAGTGAAG 
CTCAAGGGTT GTCCCAATGA GCCAAACTTT GGTTCCCTTT CTGCCCTGGT CTATCAGCAC 
TCCGTCATCC CACTGGCCCT GCCCTGTAAA CTGGTCATTC CAAGCCGAGA CCCCACAGAT 
GAATCAAAAG ATAGCTCAGG CCCTGCCAAC TCAACCACTG ACCTGCTGAA GCAAGGAGCA 
GCTTGCAATG TGCTCTTCGT CAACTCTGTG GATATGGAGT CACTCACCGG GCCACAGGCC 
ATTTCCAAAG CCACATCTGA GACTCTGGCT GCCGACCCCA CACCGGCTGC CACCATTGTT 
CACTTCAAGG TCTCTGCCCA AGGAATCACA CTGACTGACA ATCAAAGAAA GCTCTTCTTT 
AGAAGACACT ACCCCCTCAA CACTGTCACC TTCTGTGACC TGGATCCACA AGAAAGAAAG 
TGGATGAAGA CAGAGGGAGG CGCCCCTGCT AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG 
GGCAGCACCA CAGACAACGC TTGCCACCTC TTCGCAGAGC TTGACCCCAA CCAGCCTGCG 
TCTGCCATCG TCAACTTCGT CTCCAAGGTC ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_017319862.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175351.1 Mus musculus tensin 1 (Tns1), transcript variant X10, mRNA.

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CloneID	OMu77079
Clone ID Related Accession (Same CDS sequence)	XM_017319862.2 , XM_017319862.1
Accession Version	XM_017319862.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCGAC TGAGCTGGTG CTTCTCCTGC GTGATCCGCT GGGGCAAGTA CCTCTTCTCT 
TGCCTCCTGC CCCTGCGCCT CTGCCTTCGC AGCCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGGTT TAAAAGTGGT GGGACACACC CAAGGGCCGC TGGATGGGAG CCTGTACGCC 
AAGGTGAAGA AGAAGGATTC CCTGAATGGC AGTTCGGGGC CTGTCACTAC TGCACGGCCT 
GCCCTGTCAG CCACTCCCAA CCACGTGGAA CACACCCTGT CTGTGAGCAG CGACTCTGGC 
AACTCCACAG CCTCCACTAA GACAGACAAG ACCGATGAGC CAGTCTCTGG GGCTACCACT 
GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG AAAGAGCTGG ACCGCCTACT AAGTGGCTTT 
GGTGTGGATA GAGAAAAGCA AGGCGCCATG TACCGGGCTC AGCAACTCAG ATCCCATCCA 
GGCGGGGGCC CGACTGTGCC CTCCCCTGGC CGCCACATTG TCCCAGCCCA GGTTCACGTC 
AATGGTGGGG CCCTGGCATC TGAGCGGGAG ACAGACATTC TAGATGATGA GCTGCCCATC 
CAGGACGGGC AGAGTGGGGG CAGCATGGGA ACGCTGTCCT CCCTGGATGG GGTCACCAAC 
ACCAGTGAAA GTGGCTACCC AGAGACCCTG TCCCCACTGA CCAATGGCCT GGACAAACCC 
TATTCCACAG AACCTGTGCT CAACGGCGGA GGCTACCCCT ATGAGGCTGC CAACCGGGTG 
ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC ATTCGACCCT CCTACTCTGC TCAGGAGGGT 
TTAGCTGGCT ACCAGCGTGA GGGACCCCAC CCAGCCTGGT CACAGCAAGT GACCAGTGCC 
CACTGTGGCT GTGACCCCAG TGGTCTATTC AGATCCCAGT CCTTTCCAGA TGTGGAGCCC 
CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG 
AATTCATGGC AACAGCAGCA GCCCCACCCA CCTCCCCGCC AGCAGGAGAG ATCCCCACTG 
CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC CAGCTGTCAG CAGAGACCCC CGTAGCTGCT 
CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG CAGGAGATCG AGCAGTCCAT CGAAACCCTC 
AATATGTTGA TGCTGGACCT GGAGCCAGCC TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG 
AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT CCACTCTCTT CCCAGCCCCT CTTGGGGTCT 
TCCCGCCAGT CCCACCCACT TACCCAGTCC CGATCTGGCT ATATCCCCAG TGGGCATTCT 
CTGGGAACCC CGGAGCTGGT CTCTTCTGGC AGGCCTTATT CACCTTACGA TTATCAGCTG 
CATCCAGCTG GATCCAACCA GAGTTTCCAT CCAAAAAGTC CAGCCTCTTC CACCTTCCTT 
CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG GAGCCCCCAG CCTCTCTCCC TGGTCTCATC 
GCTCAGCCCC AGCTCCCACC AAAGGAAACT ACATCAGATC CCTCCCGAAC TCCAGAGGAA 
GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC CATCGGGTAG CAGTGTACAA TGCCAGACTA 
CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC 
CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG 
CGGGCAGCCA GTGATGGACA GTATGAAAAC CAGTCTCCGG AGGCCACATC CCCCCGTAGT 
CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC TCCCCTGAGC TGGCTCTTAC CATTGCCCTC 
AATCCTGGAG GGCGCCCCAA AGAGCCTCAT TTGCATAGCT ACAAGGAGGC CTTTGAGGAG 
ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA CACAGTGTGG CGCGCTCTCC TCCAGGTCTG 
GCCAAGACAC CCCTGTCTGC TCTGGGCCTG AAACCCCACA ACCCAGCAGA CATCTTGCTG 
CACCCCACAG GTGTTGCCAG AAGACTGATC CAGCCAGAGG AAGACGAGGG GGAGGAGGTG 
ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA 
GGGCCTCGAG CTCAGGACGT TGAGCCTAAG AGCTTCAGCG CACCAGCTGC TCACGCCTAT 
GGGCACGAGA CACCCCTGAG GAACGGGACC CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC 
CTCTCCACAA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CCGTGGGCAG TTTCCCATCA 
GTGGTGAGCA GTGACCAGGG TCCCCGGACA CCCTTCCAGC CTATGCTGGA CTCCAGCATC 
CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT GCAGCACTGA GTTATCAGAG CTCCTCGCCT 
GTCCCTGTTG GTGGCAGCAG CTACAACAGC CCTGACTACT CCCTCCAACC ATTCAGCTCT 
TCTCCAGAAA GCCAAGGCCA GCCACAGTAC AGTGCGGCCA GTGTCCACAT GGTACCTGGG 
AGCCCTCAGG CACGACACAG GACAGTGGGT ACCAACACTC CACCTAGCCC TGGTTTTGGC 
CGGCGGGCCG TCAACCCCAC CATGGCTGCC CCTGGTAGTC CCAGTTTGAG CCATCGGCAG 
GTGATGGGTC CATCTGGTCC AGGTTTCCAT GGTAACGTGG TTTCTGGCCA CCCAGCCAGT 
GCAGCTACCA CTCCTGGAAG CCCCAGCCTG GGCCGGCACC CAGTGGGAAG CCATCAAGTT 
CCAGGCCTCC ACAGCAGTGT GGTCACCACT CCGGGAAGCC CAAGCCTGGG CCGACACCCT 
GGTGCCCACC AAGGGAATCT GGCCTCCAGT CTCCACAGCA ATGCAGTCAT CAGCCCTGGG 
AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG TCTGGATCCG TGGTTCCTGG AAGCCCCAGC 
TTGGACCGGC ATGCAGCTTA TGGTGGCTAC TCTACCCCTG AGGACCGAAG ACCAACACTG 
TCCCGGCAAA GCAGTGCCTC TGGCTACCAG GCCCCATCCA CACCGTCATT CCCTGTCTCT 
CCTGCCTACT ACCCTGGCCT GAGCAGCCCT GCCACCTCAC CCTCGCCAGA CTCAGCAGCC 
TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA CCAGAGAAGC GAAGAATGTC AGTGGGAGAC 
CGGGCAGGCA GCCTCCCCAA CTATGCCACC ATCAATGGGA AAGTGTCTTC CTCGCCTGTC 
GCCAATGGCA TGGCCAGTGG GAGCAGCACC GTCTCCTTCT CTCACACGCT TCCGGACTTT 
TCTAAGTATT CGATGCCAGA CAATAGTCCG GAGACTCGGG CTAAAGTGAA GTTTGTCCAG 
GACACTTCCA AGTATTGGTA CAAGCCTGAG ATCTCCAGAG AACAGGCCAT CGCACTCCTA 
AAGGACCAGG AGCCAGGAGC CTTCATCATC CGGGACAGCC ACTCCTTCCG AGGGGCTTAT 
GGGCTGGCCA TGAAGGTGTC CTCACCCCCT CCAACCATCA CTCAGCAGGG CAAGAAAGGA 
GACATGACCC ATGAGCTGGT GAGGCACTTT CTGATCGAGA CAGGCCCTAG AGGAGTGAAG 
CTCAAGGGTT GTCCCAATGA GCCAAACTTT GGTTCCCTTT CTGCCCTGGT CTATCAGCAC 
TCCGTCATCC CACTGGCCCT GCCCTGTAAA CTGGTCATTC CAAGCCGAGA CCCCACAGAT 
GAATCAAAAG ATAGCTCAGG CCCTGCCAAC TCAACCACTG ACCTGCTGAA GCAAGGAGCA 
GCTTGCAATG TGCTCTTCGT CAACTCTGTG GATATGGAGT CACTCACCGG GCCACAGGCC 
ATTTCCAAAG CCACATCTGA GACTCTGGCT GCCGACCCCA CACCGGCTGC CACCATTGTT 
CACTTCAAGG TCTCTGCCCA AGGAATCACA CTGACTGACA ATCAAAGAAA GCTCTTCTTT 
AGAAGACACT ACCCCCTCAA CACTGTCACC TTCTGTGACC TGGATCCACA AGAAAGAAAG 
TGGATGAAGA CAGAGGGAGG CGCCCCTGCT AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG 
GGCAGCACCA CAGACAACGC TTGCCACCTC TTCGCAGAGC TTGACCCCAA CCAGCCTGCG 
TCTGCCATCG TCAACTTCGT CTCCAAGGTC ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175351.1
CDS	16..5712
Translation	MTRLSWCFSCVIRWGKYLFSCLLPLRLCLRSQPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_017319862.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319862.1

ORF Insert Sequence:

ATGACTCGAC TGAGCTGGTG CTTCTCCTGC GTGATCCGCT GGGGCAAGTA CCTCTTCTCT 
TGCCTCCTGC CCCTGCGCCT CTGCCTTCGC AGCCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGGTT TAAAAGTGGT GGGACACACC CAAGGGCCGC TGGATGGGAG CCTGTACGCC 
AAGGTGAAGA AGAAGGATTC CCTGAATGGC AGTTCGGGGC CTGTCACTAC TGCACGGCCT 
GCCCTGTCAG CCACTCCCAA CCACGTGGAA CACACCCTGT CTGTGAGCAG CGACTCTGGC 
AACTCCACAG CCTCCACTAA GACAGACAAG ACCGATGAGC CAGTCTCTGG GGCTACCACT 
GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG AAAGAGCTGG ACCGCCTACT AAGTGGCTTT 
GGTGTGGATA GAGAAAAGCA AGGCGCCATG TACCGGGCTC AGCAACTCAG ATCCCATCCA 
GGCGGGGGCC CGACTGTGCC CTCCCCTGGC CGCCACATTG TCCCAGCCCA GGTTCACGTC 
AATGGTGGGG CCCTGGCATC TGAGCGGGAG ACAGACATTC TAGATGATGA GCTGCCCATC 
CAGGACGGGC AGAGTGGGGG CAGCATGGGA ACGCTGTCCT CCCTGGATGG GGTCACCAAC 
ACCAGTGAAA GTGGCTACCC AGAGACCCTG TCCCCACTGA CCAATGGCCT GGACAAACCC 
TATTCCACAG AACCTGTGCT CAACGGCGGA GGCTACCCCT ATGAGGCTGC CAACCGGGTG 
ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC ATTCGACCCT CCTACTCTGC TCAGGAGGGT 
TTAGCTGGCT ACCAGCGTGA GGGACCCCAC CCAGCCTGGT CACAGCAAGT GACCAGTGCC 
CACTGTGGCT GTGACCCCAG TGGTCTATTC AGATCCCAGT CCTTTCCAGA TGTGGAGCCC 
CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG 
AATTCATGGC AACAGCAGCA GCCCCACCCA CCTCCCCGCC AGCAGGAGAG ATCCCCACTG 
CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC CAGCTGTCAG CAGAGACCCC CGTAGCTGCT 
CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG CAGGAGATCG AGCAGTCCAT CGAAACCCTC 
AATATGTTGA TGCTGGACCT GGAGCCAGCC TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG 
AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT CCACTCTCTT CCCAGCCCCT CTTGGGGTCT 
TCCCGCCAGT CCCACCCACT TACCCAGTCC CGATCTGGCT ATATCCCCAG TGGGCATTCT 
CTGGGAACCC CGGAGCTGGT CTCTTCTGGC AGGCCTTATT CACCTTACGA TTATCAGCTG 
CATCCAGCTG GATCCAACCA GAGTTTCCAT CCAAAAAGTC CAGCCTCTTC CACCTTCCTT 
CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG GAGCCCCCAG CCTCTCTCCC TGGTCTCATC 
GCTCAGCCCC AGCTCCCACC AAAGGAAACT ACATCAGATC CCTCCCGAAC TCCAGAGGAA 
GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC CATCGGGTAG CAGTGTACAA TGCCAGACTA 
CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC 
CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG 
CGGGCAGCCA GTGATGGACA GTATGAAAAC CAGTCTCCGG AGGCCACATC CCCCCGTAGT 
CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC TCCCCTGAGC TGGCTCTTAC CATTGCCCTC 
AATCCTGGAG GGCGCCCCAA AGAGCCTCAT TTGCATAGCT ACAAGGAGGC CTTTGAGGAG 
ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA CACAGTGTGG CGCGCTCTCC TCCAGGTCTG 
GCCAAGACAC CCCTGTCTGC TCTGGGCCTG AAACCCCACA ACCCAGCAGA CATCTTGCTG 
CACCCCACAG GTGTTGCCAG AAGACTGATC CAGCCAGAGG AAGACGAGGG GGAGGAGGTG 
ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA 
GGGCCTCGAG CTCAGGACGT TGAGCCTAAG AGCTTCAGCG CACCAGCTGC TCACGCCTAT 
GGGCACGAGA CACCCCTGAG GAACGGGACC CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC 
CTCTCCACAA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CCGTGGGCAG TTTCCCATCA 
GTGGTGAGCA GTGACCAGGG TCCCCGGACA CCCTTCCAGC CTATGCTGGA CTCCAGCATC 
CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT GCAGCACTGA GTTATCAGAG CTCCTCGCCT 
GTCCCTGTTG GTGGCAGCAG CTACAACAGC CCTGACTACT CCCTCCAACC ATTCAGCTCT 
TCTCCAGAAA GCCAAGGCCA GCCACAGTAC AGTGCGGCCA GTGTCCACAT GGTACCTGGG 
AGCCCTCAGG CACGACACAG GACAGTGGGT ACCAACACTC CACCTAGCCC TGGTTTTGGC 
CGGCGGGCCG TCAACCCCAC CATGGCTGCC CCTGGTAGTC CCAGTTTGAG CCATCGGCAG 
GTGATGGGTC CATCTGGTCC AGGTTTCCAT GGTAACGTGG TTTCTGGCCA CCCAGCCAGT 
GCAGCTACCA CTCCTGGAAG CCCCAGCCTG GGCCGGCACC CAGTGGGAAG CCATCAAGTT 
CCAGGCCTCC ACAGCAGTGT GGTCACCACT CCGGGAAGCC CAAGCCTGGG CCGACACCCT 
GGTGCCCACC AAGGGAATCT GGCCTCCAGT CTCCACAGCA ATGCAGTCAT CAGCCCTGGG 
AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG TCTGGATCCG TGGTTCCTGG AAGCCCCAGC 
TTGGACCGGC ATGCAGCTTA TGGTGGCTAC TCTACCCCTG AGGACCGAAG ACCAACACTG 
TCCCGGCAAA GCAGTGCCTC TGGCTACCAG GCCCCATCCA CACCGTCATT CCCTGTCTCT 
CCTGCCTACT ACCCTGGCCT GAGCAGCCCT GCCACCTCAC CCTCGCCAGA CTCAGCAGCC 
TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA CCAGAGAAGC GAAGAATGTC AGTGGGAGAC 
CGGGCAGGCA GCCTCCCCAA CTATGCCACC ATCAATGGGA AAGTGTCTTC CTCGCCTGTC 
GCCAATGGCA TGGCCAGTGG GAGCAGCACC GTCTCCTTCT CTCACACGCT TCCGGACTTT 
TCTAAGTATT CGATGCCAGA CAATAGTCCG GAGACTCGGG CTAAAGTGAA GTTTGTCCAG 
GACACTTCCA AGTATTGGTA CAAGCCTGAG ATCTCCAGAG AACAGGCCAT CGCACTCCTA 
AAGGACCAGG AGCCAGGAGC CTTCATCATC CGGGACAGCC ACTCCTTCCG AGGGGCTTAT 
GGGCTGGCCA TGAAGGTGTC CTCACCCCCT CCAACCATCA CTCAGCAGGG CAAGAAAGGA 
GACATGACCC ATGAGCTGGT GAGGCACTTT CTGATCGAGA CAGGCCCTAG AGGAGTGAAG 
CTCAAGGGTT GTCCCAATGA GCCAAACTTT GGTTCCCTTT CTGCCCTGGT CTATCAGCAC 
TCCGTCATCC CACTGGCCCT GCCCTGTAAA CTGGTCATTC CAAGCCGAGA CCCCACAGAT 
GAATCAAAAG ATAGCTCAGG CCCTGCCAAC TCAACCACTG ACCTGCTGAA GCAAGGAGCA 
GCTTGCAATG TGCTCTTCGT CAACTCTGTG GATATGGAGT CACTCACCGG GCCACAGGCC 
ATTTCCAAAG CCACATCTGA GACTCTGGCT GCCGACCCCA CACCGGCTGC CACCATTGTT 
CACTTCAAGG TCTCTGCCCA AGGAATCACA CTGACTGACA ATCAAAGAAA GCTCTTCTTT 
AGAAGACACT ACCCCCTCAA CACTGTCACC TTCTGTGACC TGGATCCACA AGAAAGAAAG 
TGGATGAAGA CAGAGGGAGG CGCCCCTGCT AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG 
GGCAGCACCA CAGACAACGC TTGCCACCTC TTCGCAGAGC TTGACCCCAA CCAGCCTGCG 
TCTGCCATCG TCAACTTCGT CTCCAAGGTC ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_017319863.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175352.1 Mus musculus tensin 1 (Tns1), transcript variant X12, mRNA.

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CloneID	OMu77080
Clone ID Related Accession (Same CDS sequence)	XM_017319863.1
Accession Version	XM_017319863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGGGGTT 
CAGGCTCGGG AGAGGCAGCC TGCAGAGCCC CCAGGCCCAC TCCGGAGGCG GGCAGCCAGT 
GATGGACAGT ATGAAAACCA GTCTCCGGAG GCCACATCCC CCCGTAGTCC TGGGGTCCGC 
TCCCCCGTCC AGTGCGTCTC CCCTGAGCTG GCTCTTACCA TTGCCCTCAA TCCTGGAGGG 
CGCCCCAAAG AGCCTCATTT GCATAGCTAC AAGGAGGCCT TTGAGGAGAT GGAGGGGACC 
TCCCCAAGCA GCCCGCCACA CAGTGTGGCG CGCTCTCCTC CAGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCCCACAAC CCAGCAGACA TCTTGCTGCA CCCCACAGGT 
GAGCCCCGGA GCTATGTGGA GTCCGTGGCG AGGACAGCGG TGGCAGGGCC TCGAGCTCAG 
GACGTTGAGC CTAAGAGCTT CAGCGCACCA GCTGCTCACG CCTATGGGCA CGAGACACCC 
CTGAGGAACG GGACCCCAGG GGGCTCCTTT GTCTCCCCGA GCCCCCTCTC CACAAGCAGC 
CCCATCCTCA GTGCTGACAG CACTTCCGTG GGCAGTTTCC CATCAGTGGT GAGCAGTGAC 
CAGGGTCCCC GGACACCCTT CCAGCCTATG CTGGACTCCA GCATCCGCTC AGGCAGCTTG 
GGGCAGCCGA GCCCTGCAGC ACTGAGTTAT CAGAGCTCCT CGCCTGTCCC TGTTGGTGGC 
AGCAGCTACA ACAGCCCTGA CTACTCCCTC CAACCATTCA GCTCTTCTCC AGAAAGCCAA 
GGCCAGCCAC AGTACAGTGC GGCCAGTGTC CACATGGTAC CTGGGAGCCC TCAGGCACGA 
CACAGGACAG TGGGTACCAA CACTCCACCT AGCCCTGGTT TTGGCCGGCG GGCCGTCAAC 
CCCACCATGG CTGCCCCTGG TAGTCCCAGT TTGAGCCATC GGCAGGTGAT GGGTCCATCT 
GGTCCAGGTT TCCATGGTAA CGTGGTTTCT GGCCACCCAG CCAGTGCAGC TACCACTCCT 
GGAAGCCCCA GCCTGGGCCG GCACCCAGTG GGAAGCCATC AAGTTCCAGG CCTCCACAGC 
AGTGTGGTCA CCACTCCGGG AAGCCCAAGC CTGGGCCGAC ACCCTGGTGC CCACCAAGGG 
AATCTGGCCT CCAGTCTCCA CAGCAATGCA GTCATCAGCC CTGGGAGCCC AAGCTTGGGC 
CGGCACCTGG GTGGGTCTGG ATCCGTGGTT CCTGGAAGCC CCAGCTTGGA CCGGCATGCA 
GCTTATGGTG GCTACTCTAC CCCTGAGGAC CGAAGACCAA CACTGTCCCG GCAAAGCAGT 
GCCTCTGGCT ACCAGGCCCC ATCCACACCG TCATTCCCTG TCTCTCCTGC CTACTACCCT 
GGCCTGAGCA GCCCTGCCAC CTCACCCTCG CCAGACTCAG CAGCCTTCCG GCAGGGGAGC 
CCCACACCAG CCTTACCAGA GAAGCGAAGA ATGTCAGTGG GAGACCGGGC AGGCAGCCTC 
CCCAACTATG CCACCATCAA TGGGAAAGTG TCTTCCTCGC CTGTCGCCAA TGGCATGGCC 
AGTGGGAGCA GCACCGTCTC CTTCTCTCAC ACGCTTCCGG ACTTTTCTAA GTATTCGATG 
CCAGACAATA GTCCGGAGAC TCGGGCTAAA GTGAAGTTTG TCCAGGACAC TTCCAAGTAT 
TGGTACAAGC CTGAGATCTC CAGAGAACAG GCCATCGCAC TCCTAAAGGA CCAGGAGCCA 
GGAGCCTTCA TCATCCGGGA CAGCCACTCC TTCCGAGGGG CTTATGGGCT GGCCATGAAG 
GTGTCCTCAC CCCCTCCAAC CATCACTCAG CAGGGCAAGA AAGGAGACAT GACCCATGAG 
CTGGTGAGGC ACTTTCTGAT CGAGACAGGC CCTAGAGGAG TGAAGCTCAA GGGTTGTCCC 
AATGAGCCAA ACTTTGGTTC CCTTTCTGCC CTGGTCTATC AGCACTCCGT CATCCCACTG 
GCCCTGCCCT GTAAACTGGT CATTCCAAGC CGAGACCCCA CAGATGAATC AAAAGATAGC 
TCAGGCCCTG CCAACTCAAC CACTGACCTG CTGAAGCAAG GAGCAGCTTG CAATGTGCTC 
TTCGTCAACT CTGTGGATAT GGAGTCACTC ACCGGGCCAC AGGCCATTTC CAAAGCCACA 
TCTGAGACTC TGGCTGCCGA CCCCACACCG GCTGCCACCA TTGTTCACTT CAAGGTCTCT 
GCCCAAGGAA TCACACTGAC TGACAATCAA AGAAAGCTCT TCTTTAGAAG ACACTACCCC 
CTCAACACTG TCACCTTCTG TGACCTGGAT CCACAAGAAA GAAAGTGGAT GAAGACAGAG 
GGAGGCGCCC CTGCTAAGCT CTTTGGCTTC GTGGCCCGGA AGCAGGGCAG CACCACAGAC 
AACGCTTGCC ACCTCTTCGC AGAGCTTGAC CCCAACCAGC CTGCGTCTGC CATCGTCAAC 
TTCGTCTCCA AGGTCATGCT GAGTGCTGGC CAGAAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175352.1
CDS	16..5637
Translation	MLGDWGKGRGQPTGSPCPPWEHILFWNLGVSLPPCCSRESGVPWTQGALRRKLLVLPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_017319863.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319863.1

ORF Insert Sequence:

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGGGGTT 
CAGGCTCGGG AGAGGCAGCC TGCAGAGCCC CCAGGCCCAC TCCGGAGGCG GGCAGCCAGT 
GATGGACAGT ATGAAAACCA GTCTCCGGAG GCCACATCCC CCCGTAGTCC TGGGGTCCGC 
TCCCCCGTCC AGTGCGTCTC CCCTGAGCTG GCTCTTACCA TTGCCCTCAA TCCTGGAGGG 
CGCCCCAAAG AGCCTCATTT GCATAGCTAC AAGGAGGCCT TTGAGGAGAT GGAGGGGACC 
TCCCCAAGCA GCCCGCCACA CAGTGTGGCG CGCTCTCCTC CAGGTCTGGC CAAGACACCC 
CTGTCTGCTC TGGGCCTGAA ACCCCACAAC CCAGCAGACA TCTTGCTGCA CCCCACAGGT 
GAGCCCCGGA GCTATGTGGA GTCCGTGGCG AGGACAGCGG TGGCAGGGCC TCGAGCTCAG 
GACGTTGAGC CTAAGAGCTT CAGCGCACCA GCTGCTCACG CCTATGGGCA CGAGACACCC 
CTGAGGAACG GGACCCCAGG GGGCTCCTTT GTCTCCCCGA GCCCCCTCTC CACAAGCAGC 
CCCATCCTCA GTGCTGACAG CACTTCCGTG GGCAGTTTCC CATCAGTGGT GAGCAGTGAC 
CAGGGTCCCC GGACACCCTT CCAGCCTATG CTGGACTCCA GCATCCGCTC AGGCAGCTTG 
GGGCAGCCGA GCCCTGCAGC ACTGAGTTAT CAGAGCTCCT CGCCTGTCCC TGTTGGTGGC 
AGCAGCTACA ACAGCCCTGA CTACTCCCTC CAACCATTCA GCTCTTCTCC AGAAAGCCAA 
GGCCAGCCAC AGTACAGTGC GGCCAGTGTC CACATGGTAC CTGGGAGCCC TCAGGCACGA 
CACAGGACAG TGGGTACCAA CACTCCACCT AGCCCTGGTT TTGGCCGGCG GGCCGTCAAC 
CCCACCATGG CTGCCCCTGG TAGTCCCAGT TTGAGCCATC GGCAGGTGAT GGGTCCATCT 
GGTCCAGGTT TCCATGGTAA CGTGGTTTCT GGCCACCCAG CCAGTGCAGC TACCACTCCT 
GGAAGCCCCA GCCTGGGCCG GCACCCAGTG GGAAGCCATC AAGTTCCAGG CCTCCACAGC 
AGTGTGGTCA CCACTCCGGG AAGCCCAAGC CTGGGCCGAC ACCCTGGTGC CCACCAAGGG 
AATCTGGCCT CCAGTCTCCA CAGCAATGCA GTCATCAGCC CTGGGAGCCC AAGCTTGGGC 
CGGCACCTGG GTGGGTCTGG ATCCGTGGTT CCTGGAAGCC CCAGCTTGGA CCGGCATGCA 
GCTTATGGTG GCTACTCTAC CCCTGAGGAC CGAAGACCAA CACTGTCCCG GCAAAGCAGT 
GCCTCTGGCT ACCAGGCCCC ATCCACACCG TCATTCCCTG TCTCTCCTGC CTACTACCCT 
GGCCTGAGCA GCCCTGCCAC CTCACCCTCG CCAGACTCAG CAGCCTTCCG GCAGGGGAGC 
CCCACACCAG CCTTACCAGA GAAGCGAAGA ATGTCAGTGG GAGACCGGGC AGGCAGCCTC 
CCCAACTATG CCACCATCAA TGGGAAAGTG TCTTCCTCGC CTGTCGCCAA TGGCATGGCC 
AGTGGGAGCA GCACCGTCTC CTTCTCTCAC ACGCTTCCGG ACTTTTCTAA GTATTCGATG 
CCAGACAATA GTCCGGAGAC TCGGGCTAAA GTGAAGTTTG TCCAGGACAC TTCCAAGTAT 
TGGTACAAGC CTGAGATCTC CAGAGAACAG GCCATCGCAC TCCTAAAGGA CCAGGAGCCA 
GGAGCCTTCA TCATCCGGGA CAGCCACTCC TTCCGAGGGG CTTATGGGCT GGCCATGAAG 
GTGTCCTCAC CCCCTCCAAC CATCACTCAG CAGGGCAAGA AAGGAGACAT GACCCATGAG 
CTGGTGAGGC ACTTTCTGAT CGAGACAGGC CCTAGAGGAG TGAAGCTCAA GGGTTGTCCC 
AATGAGCCAA ACTTTGGTTC CCTTTCTGCC CTGGTCTATC AGCACTCCGT CATCCCACTG 
GCCCTGCCCT GTAAACTGGT CATTCCAAGC CGAGACCCCA CAGATGAATC AAAAGATAGC 
TCAGGCCCTG CCAACTCAAC CACTGACCTG CTGAAGCAAG GAGCAGCTTG CAATGTGCTC 
TTCGTCAACT CTGTGGATAT GGAGTCACTC ACCGGGCCAC AGGCCATTTC CAAAGCCACA 
TCTGAGACTC TGGCTGCCGA CCCCACACCG GCTGCCACCA TTGTTCACTT CAAGGTCTCT 
GCCCAAGGAA TCACACTGAC TGACAATCAA AGAAAGCTCT TCTTTAGAAG ACACTACCCC 
CTCAACACTG TCACCTTCTG TGACCTGGAT CCACAAGAAA GAAAGTGGAT GAAGACAGAG 
GGAGGCGCCC CTGCTAAGCT CTTTGGCTTC GTGGCCCGGA AGCAGGGCAG CACCACAGAC 
AACGCTTGCC ACCTCTTCGC AGAGCTTGAC CCCAACCAGC CTGCGTCTGC CATCGTCAAC 
TTCGTCTCCA AGGTCATGCT GAGTGCTGGC CAGAAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_017319864.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175353.1 Mus musculus tensin 1 (Tns1), transcript variant X13, mRNA.

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CloneID	OMu77081
Clone ID Related Accession (Same CDS sequence)	XM_017319864.1
Accession Version	XM_017319864.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGA AGTGGTGGGA CACACCCAAG GGCCGCTGGA TGGGAGCCTG 
TACGCCAAGG TGAAGAAGAA GGATTCCCTG AATGGCAGTT CGGGGCCTGT CACTACTGCA 
CGGCCTGCCC TGTCAGCCAC TCCCAACCAC GTGGAACACA CCCTGTCTGT GAGCAGCGAC 
TCTGGCAACT CCACAGCCTC CACTAAGACA GACAAGACCG ATGAGCCAGT CTCTGGGGCT 
ACCACTGCCC CTGCTGCCCT GAGTCCTCAG GAGAAGAAAG AGCTGGACCG CCTACTAAGT 
GGCTTTGGTG TGGATAGAGA AAAGCAAGGC GCCATGTACC GGGCTCAGCA ACTCAGATCC 
CATCCAGGCG GGGGCCCGAC TGTGCCCTCC CCTGGCCGCC ACATTGTCCC AGCCCAGGTT 
CACGTCAATG GTGGGGCCCT GGCATCTGAG CGGGAGACAG ACATTCTAGA TGATGAGCTG 
CCCATCCAGG ACGGGCAGAG TGGGGGCAGC ATGGGAACGC TGTCCTCCCT GGATGGGGTC 
ACCAACACCA GTGAAAGTGG CTACCCAGAG ACCCTGTCCC CACTGACCAA TGGCCTGGAC 
AAACCCTATT CCACAGAACC TGTGCTCAAC GGCGGAGGCT ACCCCTATGA GGCTGCCAAC 
CGGGTGATTC CTGTCCACAG CAGTCACTCG GCCCCCATTC GACCCTCCTA CTCTGCTCAG 
GAGGGTTTAG CTGGCTACCA GCGTGAGGGA CCCCACCCAG CCTGGTCACA GCAAGTGACC 
AGTGCCCACT GTGGCTGTGA CCCCAGTGGT CTATTCAGAT CCCAGTCCTT TCCAGATGTG 
GAGCCCCAGC TGCCCCAAGC TCCAACCCGT GGGGGTAGTA GCCGAGAGGC TGTGCAGAGG 
GGTCTGAATT CATGGCAACA GCAGCAGCCC CACCCACCTC CCCGCCAGCA GGAGAGATCC 
CCACTGCAGA GTCTTGCCCG CAGCAAGCCT AGCCCCCAGC TGTCAGCAGA GACCCCCGTA 
GCTGCTCTCC CAGAGTTCCC AAGGGCAGCG TCCCAGCAGG AGATCGAGCA GTCCATCGAA 
ACCCTCAATA TGTTGATGCT GGACCTGGAG CCAGCCTCCG CAGCTGCCCC TCTTCATAAG 
TCCCAGAGTG TCCCTGGGGC CTGGCCAGGG GCTTCTCCAC TCTCTTCCCA GCCCCTCTTG 
GGGTCTTCCC GCCAGTCCCA CCCACTTACC CAGTCCCGAT CTGGCTATAT CCCCAGTGGG 
CATTCTCTGG GAACCCCGGA GCTGGTCTCT TCTGGCAGGC CTTATTCACC TTACGATTAT 
CAGCTGCATC CAGCTGGATC CAACCAGAGT TTCCATCCAA AAAGTCCAGC CTCTTCCACC 
TTCCTTCCAA GCCCTCATAG CTCTGCGGGG CCTCAGGAGC CCCCAGCCTC TCTCCCTGGT 
CTCATCGCTC AGCCCCAGCT CCCACCAAAG GAAACTACAT CAGATCCCTC CCGAACTCCA 
GAGGAAGAGC CGCTGAACCT GGAAGGGCTG GTAGCCCATC GGGTAGCAGG GGTTCAGGCT 
CGGGAGAGGC AGCCTGCAGA GCCCCCAGGC CCACTCCGGA GGCGGGCAGC CAGTGATGGA 
CAGTATGAAA ACCAGTCTCC GGAGGCCACA TCCCCCCGTA GTCCTGGGGT CCGCTCCCCC 
GTCCAGTGCG TCTCCCCTGA GCTGGCTCTT ACCATTGCCC TCAATCCTGG AGGGCGCCCC 
AAAGAGCCTC ATTTGCATAG CTACAAGGAG GCCTTTGAGG AGATGGAGGG GACCTCCCCA 
AGCAGCCCGC CACACAGTGT GGCGCGCTCT CCTCCAGGTC TGGCCAAGAC ACCCCTGTCT 
GCTCTGGGCC TGAAACCCCA CAACCCAGCA GACATCTTGC TGCACCCCAC AGGTGAGCCC 
CGGAGCTATG TGGAGTCCGT GGCGAGGACA GCGGTGGCAG GGCCTCGAGC TCAGGACGTT 
GAGCCTAAGA GCTTCAGCGC ACCAGCTGCT CACGCCTATG GGCACGAGAC ACCCCTGAGG 
AACGGGACCC CAGGGGGCTC CTTTGTCTCC CCGAGCCCCC TCTCCACAAG CAGCCCCATC 
CTCAGTGCTG ACAGCACTTC CGTGGGCAGT TTCCCATCAG TGGTGAGCAG TGACCAGGGT 
CCCCGGACAC CCTTCCAGCC TATGCTGGAC TCCAGCATCC GCTCAGGCAG CTTGGGGCAG 
CCGAGCCCTG CAGCACTGAG TTATCAGAGC TCCTCGCCTG TCCCTGTTGG TGGCAGCAGC 
TACAACAGCC CTGACTACTC CCTCCAACCA TTCAGCTCTT CTCCAGAAAG CCAAGGCCAG 
CCACAGTACA GTGCGGCCAG TGTCCACATG GTACCTGGGA GCCCTCAGGC ACGACACAGG 
ACAGTGGGTA CCAACACTCC ACCTAGCCCT GGTTTTGGCC GGCGGGCCGT CAACCCCACC 
ATGGCTGCCC CTGGTAGTCC CAGTTTGAGC CATCGGCAGG TGATGGGTCC ATCTGGTCCA 
GGTTTCCATG GTAACGTGGT TTCTGGCCAC CCAGCCAGTG CAGCTACCAC TCCTGGAAGC 
CCCAGCCTGG GCCGGCACCC AGTGGGAAGC CATCAAGTTC CAGGCCTCCA CAGCAGTGTG 
GTCACCACTC CGGGAAGCCC AAGCCTGGGC CGACACCCTG GTGCCCACCA AGGGAATCTG 
GCCTCCAGTC TCCACAGCAA TGCAGTCATC AGCCCTGGGA GCCCAAGCTT GGGCCGGCAC 
CTGGGTGGGT CTGGATCCGT GGTTCCTGGA AGCCCCAGCT TGGACCGGCA TGCAGCTTAT 
GGTGGCTACT CTACCCCTGA GGACCGAAGA CCAACACTGT CCCGGCAAAG CAGTGCCTCT 
GGCTACCAGG CCCCATCCAC ACCGTCATTC CCTGTCTCTC CTGCCTACTA CCCTGGCCTG 
AGCAGCCCTG CCACCTCACC CTCGCCAGAC TCAGCAGCCT TCCGGCAGGG GAGCCCCACA 
CCAGCCTTAC CAGAGAAGCG AAGAATGTCA GTGGGAGACC GGGCAGGCAG CCTCCCCAAC 
TATGCCACCA TCAATGGGAA AGTGTCTTCC TCGCCTGTCG CCAATGGCAT GGCCAGTGGG 
AGCAGCACCG TCTCCTTCTC TCACACGCTT CCGGACTTTT CTAAGTATTC GATGCCAGAC 
AATAGTCCGG AGACTCGGGC TAAAGTGAAG TTTGTCCAGG ACACTTCCAA GTATTGGTAC 
AAGCCTGAGA TCTCCAGAGA ACAGGCCATC GCACTCCTAA AGGACCAGGA GCCAGGAGCC 
TTCATCATCC GGGACAGCCA CTCCTTCCGA GGGGCTTATG GGCTGGCCAT GAAGGTGTCC 
TCACCCCCTC CAACCATCAC TCAGCAGGGC AAGAAAGGAG ACATGACCCA TGAGCTGGTG 
AGGCACTTTC TGATCGAGAC AGGCCCTAGA GGAGTGAAGC TCAAGGGTTG TCCCAATGAG 
CCAAACTTTG GTTCCCTTTC TGCCCTGGTC TATCAGCACT CCGTCATCCC ACTGGCCCTG 
CCCTGTAAAC TGGTCATTCC AAGCCGAGAC CCCACAGATG AATCAAAAGA TAGCTCAGGC 
CCTGCCAACT CAACCACTGA CCTGCTGAAG CAAGGAGCAG CTTGCAATGT GCTCTTCGTC 
AACTCTGTGG ATATGGAGTC ACTCACCGGG CCACAGGCCA TTTCCAAAGC CACATCTGAG 
ACTCTGGCTG CCGACCCCAC ACCGGCTGCC ACCATTGTTC ACTTCAAGGT CTCTGCCCAA 
GGAATCACAC TGACTGACAA TCAAAGAAAG CTCTTCTTTA GAAGACACTA CCCCCTCAAC 
ACTGTCACCT TCTGTGACCT GGATCCACAA GAAAGAAAGT GGATGAAGAC AGAGGGAGGC 
GCCCCTGCTA AGCTCTTTGG CTTCGTGGCC CGGAAGCAGG GCAGCACCAC AGACAACGCT 
TGCCACCTCT TCGCAGAGCT TGACCCCAAC CAGCCTGCGT CTGCCATCGT CAACTTCGTC 
TCCAAGGTCA TGCTGAGTGC TGGCCAGAAG AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175353.1
CDS	16..5631
Translation	MLGDWGKGRGQPTGSPCPPWEHILFWNLGVSLPPCCSRESGVPWTQGALRRKLLVLPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEEVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_017319864.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319864.1

ORF Insert Sequence:

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGA AGTGGTGGGA CACACCCAAG GGCCGCTGGA TGGGAGCCTG 
TACGCCAAGG TGAAGAAGAA GGATTCCCTG AATGGCAGTT CGGGGCCTGT CACTACTGCA 
CGGCCTGCCC TGTCAGCCAC TCCCAACCAC GTGGAACACA CCCTGTCTGT GAGCAGCGAC 
TCTGGCAACT CCACAGCCTC CACTAAGACA GACAAGACCG ATGAGCCAGT CTCTGGGGCT 
ACCACTGCCC CTGCTGCCCT GAGTCCTCAG GAGAAGAAAG AGCTGGACCG CCTACTAAGT 
GGCTTTGGTG TGGATAGAGA AAAGCAAGGC GCCATGTACC GGGCTCAGCA ACTCAGATCC 
CATCCAGGCG GGGGCCCGAC TGTGCCCTCC CCTGGCCGCC ACATTGTCCC AGCCCAGGTT 
CACGTCAATG GTGGGGCCCT GGCATCTGAG CGGGAGACAG ACATTCTAGA TGATGAGCTG 
CCCATCCAGG ACGGGCAGAG TGGGGGCAGC ATGGGAACGC TGTCCTCCCT GGATGGGGTC 
ACCAACACCA GTGAAAGTGG CTACCCAGAG ACCCTGTCCC CACTGACCAA TGGCCTGGAC 
AAACCCTATT CCACAGAACC TGTGCTCAAC GGCGGAGGCT ACCCCTATGA GGCTGCCAAC 
CGGGTGATTC CTGTCCACAG CAGTCACTCG GCCCCCATTC GACCCTCCTA CTCTGCTCAG 
GAGGGTTTAG CTGGCTACCA GCGTGAGGGA CCCCACCCAG CCTGGTCACA GCAAGTGACC 
AGTGCCCACT GTGGCTGTGA CCCCAGTGGT CTATTCAGAT CCCAGTCCTT TCCAGATGTG 
GAGCCCCAGC TGCCCCAAGC TCCAACCCGT GGGGGTAGTA GCCGAGAGGC TGTGCAGAGG 
GGTCTGAATT CATGGCAACA GCAGCAGCCC CACCCACCTC CCCGCCAGCA GGAGAGATCC 
CCACTGCAGA GTCTTGCCCG CAGCAAGCCT AGCCCCCAGC TGTCAGCAGA GACCCCCGTA 
GCTGCTCTCC CAGAGTTCCC AAGGGCAGCG TCCCAGCAGG AGATCGAGCA GTCCATCGAA 
ACCCTCAATA TGTTGATGCT GGACCTGGAG CCAGCCTCCG CAGCTGCCCC TCTTCATAAG 
TCCCAGAGTG TCCCTGGGGC CTGGCCAGGG GCTTCTCCAC TCTCTTCCCA GCCCCTCTTG 
GGGTCTTCCC GCCAGTCCCA CCCACTTACC CAGTCCCGAT CTGGCTATAT CCCCAGTGGG 
CATTCTCTGG GAACCCCGGA GCTGGTCTCT TCTGGCAGGC CTTATTCACC TTACGATTAT 
CAGCTGCATC CAGCTGGATC CAACCAGAGT TTCCATCCAA AAAGTCCAGC CTCTTCCACC 
TTCCTTCCAA GCCCTCATAG CTCTGCGGGG CCTCAGGAGC CCCCAGCCTC TCTCCCTGGT 
CTCATCGCTC AGCCCCAGCT CCCACCAAAG GAAACTACAT CAGATCCCTC CCGAACTCCA 
GAGGAAGAGC CGCTGAACCT GGAAGGGCTG GTAGCCCATC GGGTAGCAGG GGTTCAGGCT 
CGGGAGAGGC AGCCTGCAGA GCCCCCAGGC CCACTCCGGA GGCGGGCAGC CAGTGATGGA 
CAGTATGAAA ACCAGTCTCC GGAGGCCACA TCCCCCCGTA GTCCTGGGGT CCGCTCCCCC 
GTCCAGTGCG TCTCCCCTGA GCTGGCTCTT ACCATTGCCC TCAATCCTGG AGGGCGCCCC 
AAAGAGCCTC ATTTGCATAG CTACAAGGAG GCCTTTGAGG AGATGGAGGG GACCTCCCCA 
AGCAGCCCGC CACACAGTGT GGCGCGCTCT CCTCCAGGTC TGGCCAAGAC ACCCCTGTCT 
GCTCTGGGCC TGAAACCCCA CAACCCAGCA GACATCTTGC TGCACCCCAC AGGTGAGCCC 
CGGAGCTATG TGGAGTCCGT GGCGAGGACA GCGGTGGCAG GGCCTCGAGC TCAGGACGTT 
GAGCCTAAGA GCTTCAGCGC ACCAGCTGCT CACGCCTATG GGCACGAGAC ACCCCTGAGG 
AACGGGACCC CAGGGGGCTC CTTTGTCTCC CCGAGCCCCC TCTCCACAAG CAGCCCCATC 
CTCAGTGCTG ACAGCACTTC CGTGGGCAGT TTCCCATCAG TGGTGAGCAG TGACCAGGGT 
CCCCGGACAC CCTTCCAGCC TATGCTGGAC TCCAGCATCC GCTCAGGCAG CTTGGGGCAG 
CCGAGCCCTG CAGCACTGAG TTATCAGAGC TCCTCGCCTG TCCCTGTTGG TGGCAGCAGC 
TACAACAGCC CTGACTACTC CCTCCAACCA TTCAGCTCTT CTCCAGAAAG CCAAGGCCAG 
CCACAGTACA GTGCGGCCAG TGTCCACATG GTACCTGGGA GCCCTCAGGC ACGACACAGG 
ACAGTGGGTA CCAACACTCC ACCTAGCCCT GGTTTTGGCC GGCGGGCCGT CAACCCCACC 
ATGGCTGCCC CTGGTAGTCC CAGTTTGAGC CATCGGCAGG TGATGGGTCC ATCTGGTCCA 
GGTTTCCATG GTAACGTGGT TTCTGGCCAC CCAGCCAGTG CAGCTACCAC TCCTGGAAGC 
CCCAGCCTGG GCCGGCACCC AGTGGGAAGC CATCAAGTTC CAGGCCTCCA CAGCAGTGTG 
GTCACCACTC CGGGAAGCCC AAGCCTGGGC CGACACCCTG GTGCCCACCA AGGGAATCTG 
GCCTCCAGTC TCCACAGCAA TGCAGTCATC AGCCCTGGGA GCCCAAGCTT GGGCCGGCAC 
CTGGGTGGGT CTGGATCCGT GGTTCCTGGA AGCCCCAGCT TGGACCGGCA TGCAGCTTAT 
GGTGGCTACT CTACCCCTGA GGACCGAAGA CCAACACTGT CCCGGCAAAG CAGTGCCTCT 
GGCTACCAGG CCCCATCCAC ACCGTCATTC CCTGTCTCTC CTGCCTACTA CCCTGGCCTG 
AGCAGCCCTG CCACCTCACC CTCGCCAGAC TCAGCAGCCT TCCGGCAGGG GAGCCCCACA 
CCAGCCTTAC CAGAGAAGCG AAGAATGTCA GTGGGAGACC GGGCAGGCAG CCTCCCCAAC 
TATGCCACCA TCAATGGGAA AGTGTCTTCC TCGCCTGTCG CCAATGGCAT GGCCAGTGGG 
AGCAGCACCG TCTCCTTCTC TCACACGCTT CCGGACTTTT CTAAGTATTC GATGCCAGAC 
AATAGTCCGG AGACTCGGGC TAAAGTGAAG TTTGTCCAGG ACACTTCCAA GTATTGGTAC 
AAGCCTGAGA TCTCCAGAGA ACAGGCCATC GCACTCCTAA AGGACCAGGA GCCAGGAGCC 
TTCATCATCC GGGACAGCCA CTCCTTCCGA GGGGCTTATG GGCTGGCCAT GAAGGTGTCC 
TCACCCCCTC CAACCATCAC TCAGCAGGGC AAGAAAGGAG ACATGACCCA TGAGCTGGTG 
AGGCACTTTC TGATCGAGAC AGGCCCTAGA GGAGTGAAGC TCAAGGGTTG TCCCAATGAG 
CCAAACTTTG GTTCCCTTTC TGCCCTGGTC TATCAGCACT CCGTCATCCC ACTGGCCCTG 
CCCTGTAAAC TGGTCATTCC AAGCCGAGAC CCCACAGATG AATCAAAAGA TAGCTCAGGC 
CCTGCCAACT CAACCACTGA CCTGCTGAAG CAAGGAGCAG CTTGCAATGT GCTCTTCGTC 
AACTCTGTGG ATATGGAGTC ACTCACCGGG CCACAGGCCA TTTCCAAAGC CACATCTGAG 
ACTCTGGCTG CCGACCCCAC ACCGGCTGCC ACCATTGTTC ACTTCAAGGT CTCTGCCCAA 
GGAATCACAC TGACTGACAA TCAAAGAAAG CTCTTCTTTA GAAGACACTA CCCCCTCAAC 
ACTGTCACCT TCTGTGACCT GGATCCACAA GAAAGAAAGT GGATGAAGAC AGAGGGAGGC 
GCCCCTGCTA AGCTCTTTGG CTTCGTGGCC CGGAAGCAGG GCAGCACCAC AGACAACGCT 
TGCCACCTCT TCGCAGAGCT TGACCCCAAC CAGCCTGCGT CTGCCATCGT CAACTTCGTC 
TCCAAGGTCA TGCTGAGTGC TGGCCAGAAG AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_017319865.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175354.1 Mus musculus tensin 1 (Tns1), transcript variant X19, mRNA.

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CloneID	OMu77082
Clone ID Related Accession (Same CDS sequence)	XM_017319865.1
Accession Version	XM_017319865.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGCGCGC 
TCTCCTCCAG GTCTGGCCAA GACACCCCTG TCTGCTCTGG GCCTGAAACC CCACAACCCA 
GCAGACATCT TGCTGCACCC CACAGGTGTT GCCAGAAGAC TGATCCAGCC AGAGGAAGAC 
GAGGGGGAGG AGGTGACTAA GCCTCCAGAA GAGCCCCGGA GCTATGTGGA GTCCGTGGCG 
AGGACAGCGG TGGCAGGGCC TCGAGCTCAG GACGTTGAGC CTAAGAGCTT CAGCGCACCA 
GCTGCTCACG CCTATGGGCA CGAGACACCC CTGAGGAACG GGACCCCAGG GGGCTCCTTT 
GTCTCCCCGA GCCCCCTCTC CACAAGCAGC CCCATCCTCA GTGCTGACAG CACTTCCGTG 
GGCAGTTTCC CATCAGTGGT GAGCAGTGAC CAGGGTCCCC GGACACCCTT CCAGCCTATG 
CTGGACTCCA GCATCCGCTC AGGCAGCTTG GGGCAGCCGA GCCCTGCAGC ACTGAGTTAT 
CAGAGCTCCT CGCCTGTCCC TGTTGGTGGC AGCAGCTACA ACAGCCCTGA CTACTCCCTC 
CAACCATTCA GCTCTTCTCC AGAAAGCCAA GGCCAGCCAC AGTACAGTGC GGCCAGTGTC 
CACATGGTAC CTGGGAGCCC TCAGGCACGA CACAGGACAG TGGGTACCAA CACTCCACCT 
AGCCCTGGTT TTGGCCGGCG GGCCGTCAAC CCCACCATGG CTGCCCCTGG TAGTCCCAGT 
TTGAGCCATC GGCAGGTGAT GGGTCCATCT GGTCCAGGTT TCCATGGTAA CGTGGTTTCT 
GGCCACCCAG CCAGTGCAGC TACCACTCCT GGAAGCCCCA GCCTGGGCCG GCACCCAGTG 
GGAAGCCATC AAGTTCCAGG CCTCCACAGC AGTGTGGTCA CCACTCCGGG AAGCCCAAGC 
CTGGGCCGAC ACCCTGGTGC CCACCAAGGG AATCTGGCCT CCAGTCTCCA CAGCAATGCA 
GTCATCAGCC CTGGGAGCCC AAGCTTGGGC CGGCACCTGG GTGGGTCTGG ATCCGTGGTT 
CCTGGAAGCC CCAGCTTGGA CCGGCATGCA GCTTATGGTG GCTACTCTAC CCCTGAGGAC 
CGAAGACCAA CACTGTCCCG GCAAAGCAGT GCCTCTGGCT ACCAGGCCCC ATCCACACCG 
TCATTCCCTG TCTCTCCTGC CTACTACCCT GGCCTGAGCA GCCCTGCCAC CTCACCCTCG 
CCAGACTCAG CAGCCTTCCG GCAGGGGAGC CCCACACCAG CCTTACCAGA GAAGCGAAGA 
ATGTCAGTGG GAGACCGGGC AGGCAGCCTC CCCAACTATG CCACCATCAA TGGGAAAGTG 
TCTTCCTCGC CTGTCGCCAA TGGCATGGCC AGTGGGAGCA GCACCGTCTC CTTCTCTCAC 
ACGCTTCCGG ACTTTTCTAA GTATTCGATG CCAGACAATA GTCCGGAGAC TCGGGCTAAA 
GTGAAGTTTG TCCAGGACAC TTCCAAGTAT TGGTACAAGC CTGAGATCTC CAGAGAACAG 
GCCATCGCAC TCCTAAAGGA CCAGGAGCCA GGAGCCTTCA TCATCCGGGA CAGCCACTCC 
TTCCGAGGGG CTTATGGGCT GGCCATGAAG GTGTCCTCAC CCCCTCCAAC CATCACTCAG 
CAGGGCAAGA AAGGAGACAT GACCCATGAG CTGGTGAGGC ACTTTCTGAT CGAGACAGGC 
CCTAGAGGAG TGAAGCTCAA GGGTTGTCCC AATGAGCCAA ACTTTGGTTC CCTTTCTGCC 
CTGGTCTATC AGCACTCCGT CATCCCACTG GCCCTGCCCT GTAAACTGGT CATTCCAAGC 
CGAGACCCCA CAGATGAATC AAAAGATAGC TCAGGCCCTG CCAACTCAAC CACTGACCTG 
CTGAAGCAAG GAGCAGCTTG CAATGTGCTC TTCGTCAACT CTGTGGATAT GGAGTCACTC 
ACCGGGCCAC AGGCCATTTC CAAAGCCACA TCTGAGACTC TGGCTGCCGA CCCCACACCG 
GCTGCCACCA TTGTTCACTT CAAGGTCTCT GCCCAAGGAA TCACACTGAC TGACAATCAA 
AGAAAGCTCT TCTTTAGAAG ACACTACCCC CTCAACACTG TCACCTTCTG TGACCTGGAT 
CCACAAGAAA GAAAGTGGAT GAAGACAGAG GGAGGCGCCC CTGCTAAGCT CTTTGGCTTC 
GTGGCCCGGA AGCAGGGCAG CACCACAGAC AACGCTTGCC ACCTCTTCGC AGAGCTTGAC 
CCCAACCAGC CTGCGTCTGC CATCGTCAAC TTCGTCTCCA AGGTCATGCT GAGTGCTGGC 
CAGAAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175354.1
CDS	16..5427
Translation	MLGDWGKGRGQPTGSPCPPWEHILFWNLGVSLPPCCSRESGVPWTQGALRRKLLVLPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_017319865.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319865.1

ORF Insert Sequence:

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGCGCGC 
TCTCCTCCAG GTCTGGCCAA GACACCCCTG TCTGCTCTGG GCCTGAAACC CCACAACCCA 
GCAGACATCT TGCTGCACCC CACAGGTGTT GCCAGAAGAC TGATCCAGCC AGAGGAAGAC 
GAGGGGGAGG AGGTGACTAA GCCTCCAGAA GAGCCCCGGA GCTATGTGGA GTCCGTGGCG 
AGGACAGCGG TGGCAGGGCC TCGAGCTCAG GACGTTGAGC CTAAGAGCTT CAGCGCACCA 
GCTGCTCACG CCTATGGGCA CGAGACACCC CTGAGGAACG GGACCCCAGG GGGCTCCTTT 
GTCTCCCCGA GCCCCCTCTC CACAAGCAGC CCCATCCTCA GTGCTGACAG CACTTCCGTG 
GGCAGTTTCC CATCAGTGGT GAGCAGTGAC CAGGGTCCCC GGACACCCTT CCAGCCTATG 
CTGGACTCCA GCATCCGCTC AGGCAGCTTG GGGCAGCCGA GCCCTGCAGC ACTGAGTTAT 
CAGAGCTCCT CGCCTGTCCC TGTTGGTGGC AGCAGCTACA ACAGCCCTGA CTACTCCCTC 
CAACCATTCA GCTCTTCTCC AGAAAGCCAA GGCCAGCCAC AGTACAGTGC GGCCAGTGTC 
CACATGGTAC CTGGGAGCCC TCAGGCACGA CACAGGACAG TGGGTACCAA CACTCCACCT 
AGCCCTGGTT TTGGCCGGCG GGCCGTCAAC CCCACCATGG CTGCCCCTGG TAGTCCCAGT 
TTGAGCCATC GGCAGGTGAT GGGTCCATCT GGTCCAGGTT TCCATGGTAA CGTGGTTTCT 
GGCCACCCAG CCAGTGCAGC TACCACTCCT GGAAGCCCCA GCCTGGGCCG GCACCCAGTG 
GGAAGCCATC AAGTTCCAGG CCTCCACAGC AGTGTGGTCA CCACTCCGGG AAGCCCAAGC 
CTGGGCCGAC ACCCTGGTGC CCACCAAGGG AATCTGGCCT CCAGTCTCCA CAGCAATGCA 
GTCATCAGCC CTGGGAGCCC AAGCTTGGGC CGGCACCTGG GTGGGTCTGG ATCCGTGGTT 
CCTGGAAGCC CCAGCTTGGA CCGGCATGCA GCTTATGGTG GCTACTCTAC CCCTGAGGAC 
CGAAGACCAA CACTGTCCCG GCAAAGCAGT GCCTCTGGCT ACCAGGCCCC ATCCACACCG 
TCATTCCCTG TCTCTCCTGC CTACTACCCT GGCCTGAGCA GCCCTGCCAC CTCACCCTCG 
CCAGACTCAG CAGCCTTCCG GCAGGGGAGC CCCACACCAG CCTTACCAGA GAAGCGAAGA 
ATGTCAGTGG GAGACCGGGC AGGCAGCCTC CCCAACTATG CCACCATCAA TGGGAAAGTG 
TCTTCCTCGC CTGTCGCCAA TGGCATGGCC AGTGGGAGCA GCACCGTCTC CTTCTCTCAC 
ACGCTTCCGG ACTTTTCTAA GTATTCGATG CCAGACAATA GTCCGGAGAC TCGGGCTAAA 
GTGAAGTTTG TCCAGGACAC TTCCAAGTAT TGGTACAAGC CTGAGATCTC CAGAGAACAG 
GCCATCGCAC TCCTAAAGGA CCAGGAGCCA GGAGCCTTCA TCATCCGGGA CAGCCACTCC 
TTCCGAGGGG CTTATGGGCT GGCCATGAAG GTGTCCTCAC CCCCTCCAAC CATCACTCAG 
CAGGGCAAGA AAGGAGACAT GACCCATGAG CTGGTGAGGC ACTTTCTGAT CGAGACAGGC 
CCTAGAGGAG TGAAGCTCAA GGGTTGTCCC AATGAGCCAA ACTTTGGTTC CCTTTCTGCC 
CTGGTCTATC AGCACTCCGT CATCCCACTG GCCCTGCCCT GTAAACTGGT CATTCCAAGC 
CGAGACCCCA CAGATGAATC AAAAGATAGC TCAGGCCCTG CCAACTCAAC CACTGACCTG 
CTGAAGCAAG GAGCAGCTTG CAATGTGCTC TTCGTCAACT CTGTGGATAT GGAGTCACTC 
ACCGGGCCAC AGGCCATTTC CAAAGCCACA TCTGAGACTC TGGCTGCCGA CCCCACACCG 
GCTGCCACCA TTGTTCACTT CAAGGTCTCT GCCCAAGGAA TCACACTGAC TGACAATCAA 
AGAAAGCTCT TCTTTAGAAG ACACTACCCC CTCAACACTG TCACCTTCTG TGACCTGGAT 
CCACAAGAAA GAAAGTGGAT GAAGACAGAG GGAGGCGCCC CTGCTAAGCT CTTTGGCTTC 
GTGGCCCGGA AGCAGGGCAG CACCACAGAC AACGCTTGCC ACCTCTTCGC AGAGCTTGAC 
CCCAACCAGC CTGCGTCTGC CATCGTCAAC TTCGTCTCCA AGGTCATGCT GAGTGCTGGC 
CAGAAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_017319866.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175355.1 Mus musculus tensin 1 (Tns1), transcript variant X21, mRNA.

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CloneID	OMu77083
Clone ID Related Accession (Same CDS sequence)	XM_017319866.1
Accession Version	XM_017319866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGAGCCC 
CGGAGCTATG TGGAGTCCGT GGCGAGGACA GCGGTGGCAG GGCCTCGAGC TCAGGACGTT 
GAGCCTAAGA GCTTCAGCGC ACCAGCTGCT CACGCCTATG GGCACGAGAC ACCCCTGAGG 
AACGGGACCC CAGGGGGCTC CTTTGTCTCC CCGAGCCCCC TCTCCACAAG CAGCCCCATC 
CTCAGTGCTG ACAGCACTTC CGTGGGCAGT TTCCCATCAG TGGTGAGCAG TGACCAGGGT 
CCCCGGACAC CCTTCCAGCC TATGCTGGAC TCCAGCATCC GCTCAGGCAG CTTGGGGCAG 
CCGAGCCCTG CAGCACTGAG TTATCAGAGC TCCTCGCCTG TCCCTGTTGG TGGCAGCAGC 
TACAACAGCC CTGACTACTC CCTCCAACCA TTCAGCTCTT CTCCAGAAAG CCAAGGCCAG 
CCACAGTACA GTGCGGCCAG TGTCCACATG GTACCTGGGA GCCCTCAGGC ACGACACAGG 
ACAGTGGGTA CCAACACTCC ACCTAGCCCT GGTTTTGGCC GGCGGGCCGT CAACCCCACC 
ATGGCTGCCC CTGGTAGTCC CAGTTTGAGC CATCGGCAGG TGATGGGTCC ATCTGGTCCA 
GGTTTCCATG GTAACGTGGT TTCTGGCCAC CCAGCCAGTG CAGCTACCAC TCCTGGAAGC 
CCCAGCCTGG GCCGGCACCC AGTGGGAAGC CATCAAGTTC CAGGCCTCCA CAGCAGTGTG 
GTCACCACTC CGGGAAGCCC AAGCCTGGGC CGACACCCTG GTGCCCACCA AGGGAATCTG 
GCCTCCAGTC TCCACAGCAA TGCAGTCATC AGCCCTGGGA GCCCAAGCTT GGGCCGGCAC 
CTGGGTGGGT CTGGATCCGT GGTTCCTGGA AGCCCCAGCT TGGACCGGCA TGCAGCTTAT 
GGTGGCTACT CTACCCCTGA GGACCGAAGA CCAACACTGT CCCGGCAAAG CAGTGCCTCT 
GGCTACCAGG CCCCATCCAC ACCGTCATTC CCTGTCTCTC CTGCCTACTA CCCTGGCCTG 
AGCAGCCCTG CCACCTCACC CTCGCCAGAC TCAGCAGCCT TCCGGCAGGG GAGCCCCACA 
CCAGCCTTAC CAGAGAAGCG AAGAATGTCA GTGGGAGACC GGGCAGGCAG CCTCCCCAAC 
TATGCCACCA TCAATGGGAA AGTGTCTTCC TCGCCTGTCG CCAATGGCAT GGCCAGTGGG 
AGCAGCACCG TCTCCTTCTC TCACACGCTT CCGGACTTTT CTAAGTATTC GATGCCAGAC 
AATAGTCCGG AGACTCGGGC TAAAGTGAAG TTTGTCCAGG ACACTTCCAA GTATTGGTAC 
AAGCCTGAGA TCTCCAGAGA ACAGGCCATC GCACTCCTAA AGGACCAGGA GCCAGGAGCC 
TTCATCATCC GGGACAGCCA CTCCTTCCGA GGGGCTTATG GGCTGGCCAT GAAGGTGTCC 
TCACCCCCTC CAACCATCAC TCAGCAGGGC AAGAAAGGAG ACATGACCCA TGAGCTGGTG 
AGGCACTTTC TGATCGAGAC AGGCCCTAGA GGAGTGAAGC TCAAGGGTTG TCCCAATGAG 
CCAAACTTTG GTTCCCTTTC TGCCCTGGTC TATCAGCACT CCGTCATCCC ACTGGCCCTG 
CCCTGTAAAC TGGTCATTCC AAGCCGAGAC CCCACAGATG AATCAAAAGA TAGCTCAGGC 
CCTGCCAACT CAACCACTGA CCTGCTGAAG CAAGGAGCAG CTTGCAATGT GCTCTTCGTC 
AACTCTGTGG ATATGGAGTC ACTCACCGGG CCACAGGCCA TTTCCAAAGC CACATCTGAG 
ACTCTGGCTG CCGACCCCAC ACCGGCTGCC ACCATTGTTC ACTTCAAGGT CTCTGCCCAA 
GGAATCACAC TGACTGACAA TCAAAGAAAG CTCTTCTTTA GAAGACACTA CCCCCTCAAC 
ACTGTCACCT TCTGTGACCT GGATCCACAA GAAAGAAAGT GGATGAAGAC AGAGGGAGGC 
GCCCCTGCTA AGCTCTTTGG CTTCGTGGCC CGGAAGCAGG GCAGCACCAC AGACAACGCT 
TGCCACCTCT TCGCAGAGCT TGACCCCAAC CAGCCTGCGT CTGCCATCGT CAACTTCGTC 
TCCAAGGTCA TGCTGAGTGC TGGCCAGAAG AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175355.1
CDS	16..5271
Translation	MLGDWGKGRGQPTGSPCPPWEHILFWNLGVSLPPCCSRESGVPWTQGALRRKLLVLPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_017319866.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319866.1

ORF Insert Sequence:

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGAGCCC 
CGGAGCTATG TGGAGTCCGT GGCGAGGACA GCGGTGGCAG GGCCTCGAGC TCAGGACGTT 
GAGCCTAAGA GCTTCAGCGC ACCAGCTGCT CACGCCTATG GGCACGAGAC ACCCCTGAGG 
AACGGGACCC CAGGGGGCTC CTTTGTCTCC CCGAGCCCCC TCTCCACAAG CAGCCCCATC 
CTCAGTGCTG ACAGCACTTC CGTGGGCAGT TTCCCATCAG TGGTGAGCAG TGACCAGGGT 
CCCCGGACAC CCTTCCAGCC TATGCTGGAC TCCAGCATCC GCTCAGGCAG CTTGGGGCAG 
CCGAGCCCTG CAGCACTGAG TTATCAGAGC TCCTCGCCTG TCCCTGTTGG TGGCAGCAGC 
TACAACAGCC CTGACTACTC CCTCCAACCA TTCAGCTCTT CTCCAGAAAG CCAAGGCCAG 
CCACAGTACA GTGCGGCCAG TGTCCACATG GTACCTGGGA GCCCTCAGGC ACGACACAGG 
ACAGTGGGTA CCAACACTCC ACCTAGCCCT GGTTTTGGCC GGCGGGCCGT CAACCCCACC 
ATGGCTGCCC CTGGTAGTCC CAGTTTGAGC CATCGGCAGG TGATGGGTCC ATCTGGTCCA 
GGTTTCCATG GTAACGTGGT TTCTGGCCAC CCAGCCAGTG CAGCTACCAC TCCTGGAAGC 
CCCAGCCTGG GCCGGCACCC AGTGGGAAGC CATCAAGTTC CAGGCCTCCA CAGCAGTGTG 
GTCACCACTC CGGGAAGCCC AAGCCTGGGC CGACACCCTG GTGCCCACCA AGGGAATCTG 
GCCTCCAGTC TCCACAGCAA TGCAGTCATC AGCCCTGGGA GCCCAAGCTT GGGCCGGCAC 
CTGGGTGGGT CTGGATCCGT GGTTCCTGGA AGCCCCAGCT TGGACCGGCA TGCAGCTTAT 
GGTGGCTACT CTACCCCTGA GGACCGAAGA CCAACACTGT CCCGGCAAAG CAGTGCCTCT 
GGCTACCAGG CCCCATCCAC ACCGTCATTC CCTGTCTCTC CTGCCTACTA CCCTGGCCTG 
AGCAGCCCTG CCACCTCACC CTCGCCAGAC TCAGCAGCCT TCCGGCAGGG GAGCCCCACA 
CCAGCCTTAC CAGAGAAGCG AAGAATGTCA GTGGGAGACC GGGCAGGCAG CCTCCCCAAC 
TATGCCACCA TCAATGGGAA AGTGTCTTCC TCGCCTGTCG CCAATGGCAT GGCCAGTGGG 
AGCAGCACCG TCTCCTTCTC TCACACGCTT CCGGACTTTT CTAAGTATTC GATGCCAGAC 
AATAGTCCGG AGACTCGGGC TAAAGTGAAG TTTGTCCAGG ACACTTCCAA GTATTGGTAC 
AAGCCTGAGA TCTCCAGAGA ACAGGCCATC GCACTCCTAA AGGACCAGGA GCCAGGAGCC 
TTCATCATCC GGGACAGCCA CTCCTTCCGA GGGGCTTATG GGCTGGCCAT GAAGGTGTCC 
TCACCCCCTC CAACCATCAC TCAGCAGGGC AAGAAAGGAG ACATGACCCA TGAGCTGGTG 
AGGCACTTTC TGATCGAGAC AGGCCCTAGA GGAGTGAAGC TCAAGGGTTG TCCCAATGAG 
CCAAACTTTG GTTCCCTTTC TGCCCTGGTC TATCAGCACT CCGTCATCCC ACTGGCCCTG 
CCCTGTAAAC TGGTCATTCC AAGCCGAGAC CCCACAGATG AATCAAAAGA TAGCTCAGGC 
CCTGCCAACT CAACCACTGA CCTGCTGAAG CAAGGAGCAG CTTGCAATGT GCTCTTCGTC 
AACTCTGTGG ATATGGAGTC ACTCACCGGG CCACAGGCCA TTTCCAAAGC CACATCTGAG 
ACTCTGGCTG CCGACCCCAC ACCGGCTGCC ACCATTGTTC ACTTCAAGGT CTCTGCCCAA 
GGAATCACAC TGACTGACAA TCAAAGAAAG CTCTTCTTTA GAAGACACTA CCCCCTCAAC 
ACTGTCACCT TCTGTGACCT GGATCCACAA GAAAGAAAGT GGATGAAGAC AGAGGGAGGC 
GCCCCTGCTA AGCTCTTTGG CTTCGTGGCC CGGAAGCAGG GCAGCACCAC AGACAACGCT 
TGCCACCTCT TCGCAGAGCT TGACCCCAAC CAGCCTGCGT CTGCCATCGT CAACTTCGTC 
TCCAAGGTCA TGCTGAGTGC TGGCCAGAAG AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_030252982.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108842.1 Mus musculus tensin 1 (Tns1), transcript variant X7, mRNA.

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CloneID	OMu85104
Clone ID Related Accession (Same CDS sequence)	XM_030252982.1
Accession Version	XM_030252982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCA CCGTGAGCCT GGTGTGCTGC GAGGCGCTCG AGCCTCTGCC CTCCTGCGGC 
CCGCAGCCCC CCGGGACTCC GCCGGGCCCC GCGCGGCCCG AGCGCTGTGA GCCCGGGGGT 
GCGGCGCCCG ACCCGAGGAG GCGGCTGCTG CTTCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGAAG TGGTGGGACA CACCCAAGGG CCGCTGGATG GGAGCCTGTA CGCCAAGGTG 
AAGAAGAAGG ATTCCCTGAA TGGCAGTTCG GGGCCTGTCA CTACTGCACG GCCTGCCCTG 
TCAGCCACTC CCAACCACGT GGAACACACC CTGTCTGTGA GCAGCGACTC TGGCAACTCC 
ACAGCCTCCA CTAAGACAGA CAAGACCGAT GAGCCAGTCT CTGGGGCTAC CACTGCCCCT 
GCTGCCCTGA GTCCTCAGGA GAAGAAAGAG CTGGACCGCC TACTAAGTGG CTTTGGTGTG 
GATAGAGAAA AGCAAGGCGC CATGTACCGG GCTCAGCAAC TCAGATCCCA TCCAGGCGGG 
GGCCCGACTG TGCCCTCCCC TGGCCGCCAC ATTGTCCCAG CCCAGGTTCA CGTCAATGGT 
GGGGCCCTGG CATCTGAGCG GGAGACAGAC ATTCTAGATG ATGAGCTGCC CATCCAGGAC 
GGGCAGAGTG GGGGCAGCAT GGGAACGCTG TCCTCCCTGG ATGGGGTCAC CAACACCAGT 
GAAAGTGGCT ACCCAGAGAC CCTGTCCCCA CTGACCAATG GCCTGGACAA ACCCTATTCC 
ACAGAACCTG TGCTCAACGG CGGAGGCTAC CCCTATGAGG CTGCCAACCG GGTGATTCCT 
GTCCACAGCA GTCACTCGGC CCCCATTCGA CCCTCCTACT CTGCTCAGGA GGGTTTAGCT 
GGCTACCAGC GTGAGGGACC CCACCCAGCC TGGTCACAGC AAGTGACCAG TGCCCACTGT 
GGCTGTGACC CCAGTGGTCT ATTCAGATCC CAGTCCTTTC CAGATGTGGA GCCCCAGCTG 
CCCCAAGCTC CAACCCGTGG GGGTAGTAGC CGAGAGGCTG TGCAGAGGGG TCTGAATTCA 
TGGCAACAGC AGCAGCCCCA CCCACCTCCC CGCCAGCAGG AGAGATCCCC ACTGCAGAGT 
CTTGCCCGCA GCAAGCCTAG CCCCCAGCTG TCAGCAGAGA CCCCCGTAGC TGCTCTCCCA 
GAGTTCCCAA GGGCAGCGTC CCAGCAGGAG ATCGAGCAGT CCATCGAAAC CCTCAATATG 
TTGATGCTGG ACCTGGAGCC AGCCTCCGCA GCTGCCCCTC TTCATAAGTC CCAGAGTGTC 
CCTGGGGCCT GGCCAGGGGC TTCTCCACTC TCTTCCCAGC CCCTCTTGGG GTCTTCCCGC 
CAGTCCCACC CACTTACCCA GTCCCGATCT GGCTATATCC CCAGTGGGCA TTCTCTGGGA 
ACCCCGGAGC TGGTCTCTTC TGGCAGGCCT TATTCACCTT ACGATTATCA GCTGCATCCA 
GCTGGATCCA ACCAGAGTTT CCATCCAAAA AGTCCAGCCT CTTCCACCTT CCTTCCAAGC 
CCTCATAGCT CTGCGGGGCC TCAGGAGCCC CCAGCCTCTC TCCCTGGTCT CATCGCTCAG 
CCCCAGCTCC CACCAAAGGA AACTACATCA GATCCCTCCC GAACTCCAGA GGAAGAGCCG 
CTGAACCTGG AAGGGCTGGT AGCCCATCGG GTAGCAGTGT ACAATGCCAG ACTACAGAGC 
CTGAGGCAGA GCCGCGGCTT CCAACCCTCT CCCCACTGGC GCCGATCTGC CTCCCTCTCT 
GGGGTTCAGG CTCGGGAGAG GCAGCCTGCA GAGCCCCCAG GCCCACTCCG GAGGCGGGCA 
GCCAGTGATG GACAGTATGA AAACCAGTCT CCGGAGGCCA CATCCCCCCG TAGTCCTGGG 
GTCCGCTCCC CCGTCCAGTG CGTCTCCCCT GAGCTGGCTC TTACCATTGC CCTCAATCCT 
GGAGGGCGCC CCAAAGAGCC TCATTTGCAT AGCTACAAGG AGGCCTTTGA GGAGATGGAG 
GGGACCTCCC CAAGCAGCCC GCCACACAGT GTGGCGCGCT CTCCTCCAGG TCTGGCCAAG 
ACACCCCTGT CTGCTCTGGG CCTGAAACCC CACAACCCAG CAGACATCTT GCTGCACCCC 
ACAGGTGTTG CCAGAAGACT GATCCAGCCA GAGGAAGACG AGGGGGAGGA GGTGACTAAG 
CCTCCAGAAG AGCCCCGGAG CTATGTGGAG TCCGTGGCGA GGACAGCGGT GGCAGGGCCT 
CGAGCTCAGG ACGTTGAGCC TAAGAGCTTC AGCGCACCAG CTGCTCACGC CTATGGGCAC 
GAGACACCCC TGAGGAACGG GACCCCAGGG GGCTCCTTTG TCTCCCCGAG CCCCCTCTCC 
ACAAGCAGCC CCATCCTCAG TGCTGACAGC ACTTCCGTGG GCAGTTTCCC ATCAGTGGTG 
AGCAGTGACC AGGGTCCCCG GACACCCTTC CAGCCTATGC TGGACTCCAG CATCCGCTCA 
GGCAGCTTGG GGCAGCCGAG CCCTGCAGCA CTGAGTTATC AGAGCTCCTC GCCTGTCCCT 
GTTGGTGGCA GCAGCTACAA CAGCCCTGAC TACTCCCTCC AACCATTCAG CTCTTCTCCA 
GAAAGCCAAG GCCAGCCACA GTACAGTGCG GCCAGTGTCC ACATGGTACC TGGGAGCCCT 
CAGGCACGAC ACAGGACAGT GGGTACCAAC ACTCCACCTA GCCCTGGTTT TGGCCGGCGG 
GCCGTCAACC CCACCATGGC TGCCCCTGGT AGTCCCAGTT TGAGCCATCG GCAGGTGATG 
GGTCCATCTG GTCCAGGTTT CCATGGTAAC GTGGTTTCTG GCCACCCAGC CAGTGCAGCT 
ACCACTCCTG GAAGCCCCAG CCTGGGCCGG CACCCAGTGG GAAGCCATCA AGTTCCAGGC 
CTCCACAGCA GTGTGGTCAC CACTCCGGGA AGCCCAAGCC TGGGCCGACA CCCTGGTGCC 
CACCAAGGGA ATCTGGCCTC CAGTCTCCAC AGCAATGCAG TCATCAGCCC TGGGAGCCCA 
AGCTTGGGCC GGCACCTGGG TGGGTCTGGA TCCGTGGTTC CTGGAAGCCC CAGCTTGGAC 
CGGCATGCAG CTTATGGTGG CTACTCTACC CCTGAGGACC GAAGACCAAC ACTGTCCCGG 
CAAAGCAGTG CCTCTGGCTA CCAGGCCCCA TCCACACCGT CATTCCCTGT CTCTCCTGCC 
TACTACCCTG GCCTGAGCAG CCCTGCCACC TCACCCTCGC CAGACTCAGC AGCCTTCCGG 
CAGGGGAGCC CCACACCAGC CTTACCAGAG AAGCGAAGAA TGTCAGTGGG AGACCGGGCA 
GGCAGCCTCC CCAACTATGC CACCATCAAT GGGAAAGTGT CTTCCTCGCC TGTCGCCAAT 
GGCATGGCCA GTGGGAGCAG CACCGTCTCC TTCTCTCACA CGCTTCCGGA CTTTTCTAAG 
TATTCGATGC CAGACAATAG TCCGGAGACT CGGGCTAAAG TGAAGTTTGT CCAGGACACT 
TCCAAGTATT GGTACAAGCC TGAGATCTCC AGAGAACAGG CCATCGCACT CCTAAAGGAC 
CAGGAGCCAG GAGCCTTCAT CATCCGGGAC AGCCACTCCT TCCGAGGGGC TTATGGGCTG 
GCCATGAAGG TGTCCTCACC CCCTCCAACC ATCACTCAGC AGGGCAAGAA AGGAGACATG 
ACCCATGAGC TGGTGAGGCA CTTTCTGATC GAGACAGGCC CTAGAGGAGT GAAGCTCAAG 
GGTTGTCCCA ATGAGCCAAA CTTTGGTTCC CTTTCTGCCC TGGTCTATCA GCACTCCGTC 
ATCCCACTGG CCCTGCCCTG TAAACTGGTC ATTCCAAGCC GAGACCCCAC AGATGAATCA 
AAAGATAGCT CAGGCCCTGC CAACTCAACC ACTGACCTGC TGAAGCAAGG AGCAGCTTGC 
AATGTGCTCT TCGTCAACTC TGTGGATATG GAGTCACTCA CCGGGCCACA GGCCATTTCC 
AAAGCCACAT CTGAGACTCT GGCTGCCGAC CCCACACCGG CTGCCACCAT TGTTCACTTC 
AAGGTCTCTG CCCAAGGAAT CACACTGACT GACAATCAAA GAAAGCTCTT CTTTAGAAGA 
CACTACCCCC TCAACACTGT CACCTTCTGT GACCTGGATC CACAAGAAAG AAAGTGGATG 
AAGACAGAGG GAGGCGCCCC TGCTAAGCTC TTTGGCTTCG TGGCCCGGAA GCAGGGCAGC 
ACCACAGACA ACGCTTGCCA CCTCTTCGCA GAGCTTGACC CCAACCAGCC TGCGTCTGCC 
ATCGTCAACT TCGTCTCCAA GGTCATGCTG AGTGCTGGCC AGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108842.1
CDS	84..5834
Translation	MGCTVSLVCCEALEPLPSCGPQPPGTPPGPARPERCEPGGAAPDPRRRLLLQPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEEVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_030252982.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252982.1

ORF Insert Sequence:

ATGGGCTGCA CCGTGAGCCT GGTGTGCTGC GAGGCGCTCG AGCCTCTGCC CTCCTGCGGC 
CCGCAGCCCC CCGGGACTCC GCCGGGCCCC GCGCGGCCCG AGCGCTGTGA GCCCGGGGGT 
GCGGCGCCCG ACCCGAGGAG GCGGCTGCTG CTTCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGAAG TGGTGGGACA CACCCAAGGG CCGCTGGATG GGAGCCTGTA CGCCAAGGTG 
AAGAAGAAGG ATTCCCTGAA TGGCAGTTCG GGGCCTGTCA CTACTGCACG GCCTGCCCTG 
TCAGCCACTC CCAACCACGT GGAACACACC CTGTCTGTGA GCAGCGACTC TGGCAACTCC 
ACAGCCTCCA CTAAGACAGA CAAGACCGAT GAGCCAGTCT CTGGGGCTAC CACTGCCCCT 
GCTGCCCTGA GTCCTCAGGA GAAGAAAGAG CTGGACCGCC TACTAAGTGG CTTTGGTGTG 
GATAGAGAAA AGCAAGGCGC CATGTACCGG GCTCAGCAAC TCAGATCCCA TCCAGGCGGG 
GGCCCGACTG TGCCCTCCCC TGGCCGCCAC ATTGTCCCAG CCCAGGTTCA CGTCAATGGT 
GGGGCCCTGG CATCTGAGCG GGAGACAGAC ATTCTAGATG ATGAGCTGCC CATCCAGGAC 
GGGCAGAGTG GGGGCAGCAT GGGAACGCTG TCCTCCCTGG ATGGGGTCAC CAACACCAGT 
GAAAGTGGCT ACCCAGAGAC CCTGTCCCCA CTGACCAATG GCCTGGACAA ACCCTATTCC 
ACAGAACCTG TGCTCAACGG CGGAGGCTAC CCCTATGAGG CTGCCAACCG GGTGATTCCT 
GTCCACAGCA GTCACTCGGC CCCCATTCGA CCCTCCTACT CTGCTCAGGA GGGTTTAGCT 
GGCTACCAGC GTGAGGGACC CCACCCAGCC TGGTCACAGC AAGTGACCAG TGCCCACTGT 
GGCTGTGACC CCAGTGGTCT ATTCAGATCC CAGTCCTTTC CAGATGTGGA GCCCCAGCTG 
CCCCAAGCTC CAACCCGTGG GGGTAGTAGC CGAGAGGCTG TGCAGAGGGG TCTGAATTCA 
TGGCAACAGC AGCAGCCCCA CCCACCTCCC CGCCAGCAGG AGAGATCCCC ACTGCAGAGT 
CTTGCCCGCA GCAAGCCTAG CCCCCAGCTG TCAGCAGAGA CCCCCGTAGC TGCTCTCCCA 
GAGTTCCCAA GGGCAGCGTC CCAGCAGGAG ATCGAGCAGT CCATCGAAAC CCTCAATATG 
TTGATGCTGG ACCTGGAGCC AGCCTCCGCA GCTGCCCCTC TTCATAAGTC CCAGAGTGTC 
CCTGGGGCCT GGCCAGGGGC TTCTCCACTC TCTTCCCAGC CCCTCTTGGG GTCTTCCCGC 
CAGTCCCACC CACTTACCCA GTCCCGATCT GGCTATATCC CCAGTGGGCA TTCTCTGGGA 
ACCCCGGAGC TGGTCTCTTC TGGCAGGCCT TATTCACCTT ACGATTATCA GCTGCATCCA 
GCTGGATCCA ACCAGAGTTT CCATCCAAAA AGTCCAGCCT CTTCCACCTT CCTTCCAAGC 
CCTCATAGCT CTGCGGGGCC TCAGGAGCCC CCAGCCTCTC TCCCTGGTCT CATCGCTCAG 
CCCCAGCTCC CACCAAAGGA AACTACATCA GATCCCTCCC GAACTCCAGA GGAAGAGCCG 
CTGAACCTGG AAGGGCTGGT AGCCCATCGG GTAGCAGTGT ACAATGCCAG ACTACAGAGC 
CTGAGGCAGA GCCGCGGCTT CCAACCCTCT CCCCACTGGC GCCGATCTGC CTCCCTCTCT 
GGGGTTCAGG CTCGGGAGAG GCAGCCTGCA GAGCCCCCAG GCCCACTCCG GAGGCGGGCA 
GCCAGTGATG GACAGTATGA AAACCAGTCT CCGGAGGCCA CATCCCCCCG TAGTCCTGGG 
GTCCGCTCCC CCGTCCAGTG CGTCTCCCCT GAGCTGGCTC TTACCATTGC CCTCAATCCT 
GGAGGGCGCC CCAAAGAGCC TCATTTGCAT AGCTACAAGG AGGCCTTTGA GGAGATGGAG 
GGGACCTCCC CAAGCAGCCC GCCACACAGT GTGGCGCGCT CTCCTCCAGG TCTGGCCAAG 
ACACCCCTGT CTGCTCTGGG CCTGAAACCC CACAACCCAG CAGACATCTT GCTGCACCCC 
ACAGGTGTTG CCAGAAGACT GATCCAGCCA GAGGAAGACG AGGGGGAGGA GGTGACTAAG 
CCTCCAGAAG AGCCCCGGAG CTATGTGGAG TCCGTGGCGA GGACAGCGGT GGCAGGGCCT 
CGAGCTCAGG ACGTTGAGCC TAAGAGCTTC AGCGCACCAG CTGCTCACGC CTATGGGCAC 
GAGACACCCC TGAGGAACGG GACCCCAGGG GGCTCCTTTG TCTCCCCGAG CCCCCTCTCC 
ACAAGCAGCC CCATCCTCAG TGCTGACAGC ACTTCCGTGG GCAGTTTCCC ATCAGTGGTG 
AGCAGTGACC AGGGTCCCCG GACACCCTTC CAGCCTATGC TGGACTCCAG CATCCGCTCA 
GGCAGCTTGG GGCAGCCGAG CCCTGCAGCA CTGAGTTATC AGAGCTCCTC GCCTGTCCCT 
GTTGGTGGCA GCAGCTACAA CAGCCCTGAC TACTCCCTCC AACCATTCAG CTCTTCTCCA 
GAAAGCCAAG GCCAGCCACA GTACAGTGCG GCCAGTGTCC ACATGGTACC TGGGAGCCCT 
CAGGCACGAC ACAGGACAGT GGGTACCAAC ACTCCACCTA GCCCTGGTTT TGGCCGGCGG 
GCCGTCAACC CCACCATGGC TGCCCCTGGT AGTCCCAGTT TGAGCCATCG GCAGGTGATG 
GGTCCATCTG GTCCAGGTTT CCATGGTAAC GTGGTTTCTG GCCACCCAGC CAGTGCAGCT 
ACCACTCCTG GAAGCCCCAG CCTGGGCCGG CACCCAGTGG GAAGCCATCA AGTTCCAGGC 
CTCCACAGCA GTGTGGTCAC CACTCCGGGA AGCCCAAGCC TGGGCCGACA CCCTGGTGCC 
CACCAAGGGA ATCTGGCCTC CAGTCTCCAC AGCAATGCAG TCATCAGCCC TGGGAGCCCA 
AGCTTGGGCC GGCACCTGGG TGGGTCTGGA TCCGTGGTTC CTGGAAGCCC CAGCTTGGAC 
CGGCATGCAG CTTATGGTGG CTACTCTACC CCTGAGGACC GAAGACCAAC ACTGTCCCGG 
CAAAGCAGTG CCTCTGGCTA CCAGGCCCCA TCCACACCGT CATTCCCTGT CTCTCCTGCC 
TACTACCCTG GCCTGAGCAG CCCTGCCACC TCACCCTCGC CAGACTCAGC AGCCTTCCGG 
CAGGGGAGCC CCACACCAGC CTTACCAGAG AAGCGAAGAA TGTCAGTGGG AGACCGGGCA 
GGCAGCCTCC CCAACTATGC CACCATCAAT GGGAAAGTGT CTTCCTCGCC TGTCGCCAAT 
GGCATGGCCA GTGGGAGCAG CACCGTCTCC TTCTCTCACA CGCTTCCGGA CTTTTCTAAG 
TATTCGATGC CAGACAATAG TCCGGAGACT CGGGCTAAAG TGAAGTTTGT CCAGGACACT 
TCCAAGTATT GGTACAAGCC TGAGATCTCC AGAGAACAGG CCATCGCACT CCTAAAGGAC 
CAGGAGCCAG GAGCCTTCAT CATCCGGGAC AGCCACTCCT TCCGAGGGGC TTATGGGCTG 
GCCATGAAGG TGTCCTCACC CCCTCCAACC ATCACTCAGC AGGGCAAGAA AGGAGACATG 
ACCCATGAGC TGGTGAGGCA CTTTCTGATC GAGACAGGCC CTAGAGGAGT GAAGCTCAAG 
GGTTGTCCCA ATGAGCCAAA CTTTGGTTCC CTTTCTGCCC TGGTCTATCA GCACTCCGTC 
ATCCCACTGG CCCTGCCCTG TAAACTGGTC ATTCCAAGCC GAGACCCCAC AGATGAATCA 
AAAGATAGCT CAGGCCCTGC CAACTCAACC ACTGACCTGC TGAAGCAAGG AGCAGCTTGC 
AATGTGCTCT TCGTCAACTC TGTGGATATG GAGTCACTCA CCGGGCCACA GGCCATTTCC 
AAAGCCACAT CTGAGACTCT GGCTGCCGAC CCCACACCGG CTGCCACCAT TGTTCACTTC 
AAGGTCTCTG CCCAAGGAAT CACACTGACT GACAATCAAA GAAAGCTCTT CTTTAGAAGA 
CACTACCCCC TCAACACTGT CACCTTCTGT GACCTGGATC CACAAGAAAG AAAGTGGATG 
AAGACAGAGG GAGGCGCCCC TGCTAAGCTC TTTGGCTTCG TGGCCCGGAA GCAGGGCAGC 
ACCACAGACA ACGCTTGCCA CCTCTTCGCA GAGCTTGACC CCAACCAGCC TGCGTCTGCC 
ATCGTCAACT TCGTCTCCAA GGTCATGCTG AGTGCTGGCC AGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_030252983.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108843.1 Mus musculus tensin 1 (Tns1), transcript variant X15, mRNA.

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CloneID	OMu85105
Clone ID Related Accession (Same CDS sequence)	XM_030252983.1
Accession Version	XM_030252983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTGGA TTTGTCTGTC CTGCATGCTC TGGCCAGAAG ACCTGGAGGC CCCCAAGACC 
CACCACTTCA AGGTGAAGGC CTTCAAGAAG GTGAAGCCCT GTGGCATCTG CCGGCAGGCC 
ATCACTCGGG AAGGCTGTGT TTGCAAAGTC TGCAGCTTCT CCTGTCATCG GAAATGCCAG 
GCCAAGGTGG CTGCCCCTTG CGTTCCTCCG TCCAGCCATG AACTGGTGCC CATCACCACA 
GAGACCGTAC CCAAGAATGT AGTAGATGTG GGGGAAGGAG ACTGCCGGGT TGGAAGTTCT 
CCGAAAAACC TGGAGGAGGG CGGCTCCATG AGAGTTTCTC CAAGCATACA GCCACAACCA 
CAATCACAGC CTACCAGTCT CTCTAGAAAC ACGAGTGTGA GCCGAGCCAT GGAAGACAGT 
TGTGAGCTGG ACCTGGTGTA TGTCACCGAA CGCATCATCG CCGTGTCCTT CCCTAGCACA 
GCCAATGAGG AGAACTTCCG CAGCAACCTC CGTGAAGTGG CTCAGATGTT GAAGTCCAAG 
CATGGGGGCA ACTACCTGCT ATTCAACCTC TCTGAACAGA GGCCTGACAT CACCAAGCTG 
CATGCTAAGG TCCTGGAATT CGGCTGGCCT GACCTGCACA CACCGGCCCT GGAGAAGATA 
TGCAGTGTCT GTAAGGCCAT GGACACCTGG CTCAATGCAG ACCCCCACAA TGTCGTTGTT 
CTCCACAACA AGGGAAACCG AGGCAGGATT GGGGTTGTCA TTGCAGCGTA TCTGCACTAC 
AGCAACATTT CAGCCAGTGC TGACCAGGCT CTGGACCGGT TTGCAATGAA GAGATTCTAT 
GAGGACAAGA TCGTGCCCAT TGGCCAGCCA TCCCAGAGGA GATATGTGCA CTACTTCAGT 
GGCCTACTCT CTGGCTCCAT CAAAATGAAC AACAAGCCTT TGTTTCTGCA TCATGTGATC 
ATGCACGGCA TCCCCAACTT TGAGTCTAAA GGAGGGTGCC GGCCCTTTCT CCGCATCTAC 
CAAGCCATGC AACCTGTGTA CACATCTGGC ATTTACAACA TCCCTGGAGA CAGCCAGGCC 
AGCATCTGTA TCACCATTGA ACCAGGGCTG CTCTTGAAGG GAGACATCTT GCTGAAGTGT 
TATCATAAGA AATTCCGGAG CCCGGCTCGA GACGTCATCT TCCGAGTGCA GTTCCACACC 
TGTGCCATCC ATGACTTGGG GGTTGTTTTT GGGAAGGAAG ATCTGGATGA GGCCTTCAAA 
GATGATCGAT TTCCTGACTA TGGCAAAGTG GAGTTTGTCT TTTCCTATGG ACCAGAGAAA 
ATTCAAGGCA TGGAACACCT GGAGAATGGG CCCAGCGTAT CTGTAGACTA TAACACTTCT 
GACCCCCTCA TCCGCTGGGA CTCCTATGAC AACTTCAGTG GGCATCGTGA GGATGGCATG 
GAAGAAGTGG TGGGACACAC CCAAGGGCCG CTGGATGGGA GCCTGTACGC CAAGGTGAAG 
AAGAAGGATT CCCTGAATGG CAGTTCGGGG CCTGTCACTA CTGCACGGCC TGCCCTGTCA 
GCCACTCCCA ACCACGTGGA ACACACCCTG TCTGTGAGCA GCGACTCTGG CAACTCCACA 
GCCTCCACTA AGACAGACAA GACCGATGAG CCAGTCTCTG GGGCTACCAC TGCCCCTGCT 
GCCCTGAGTC CTCAGGAGAA GAAAGAGCTG GACCGCCTAC TAAGTGGCTT TGGTGTGGAT 
AGAGAAAAGC AAGGCGCCAT GTACCGGGCT CAGCAACTCA GATCCCATCC AGGCGGGGGC 
CCGACTGTGC CCTCCCCTGG CCGCCACATT GTCCCAGCCC AGGTTCACGT CAATGGTGGG 
GCCCTGGCAT CTGAGCGGGA GACAGACATT CTAGATGATG AGCTGCCCAT CCAGGACGGG 
CAGAGTGGGG GCAGCATGGG AACGCTGTCC TCCCTGGATG GGGTCACCAA CACCAGTGAA 
AGTGGCTACC CAGAGACCCT GTCCCCACTG ACCAATGGCC TGGACAAACC CTATTCCACA 
GAACCTGTGC TCAACGGCGG AGGCTACCCC TATGAGGCTG CCAACCGGGT GATTCCTGTC 
CACAGCAGTC ACTCGGCCCC CATTCGACCC TCCTACTCTG CTCAGGAGGG TTTAGCTGGC 
TACCAGCGTG AGGGACCCCA CCCAGCCTGG TCACAGCAAG TGACCAGTGC CCACTGTGGC 
TGTGACCCCA GTGGTCTATT CAGATCCCAG TCCTTTCCAG ATGTGGAGCC CCAGCTGCCC 
CAAGCTCCAA CCCGTGGGGG TAGTAGCCGA GAGGCTGTGC AGAGGGGTCT GAATTCATGG 
CAACAGCAGC AGCCCCACCC ACCTCCCCGC CAGCAGGAGA GATCCCCACT GCAGAGTCTT 
GCCCGCAGCA AGCCTAGCCC CCAGCTGTCA GCAGAGACCC CCGTAGCTGC TCTCCCAGAG 
TTCCCAAGGG CAGCGTCCCA GCAGGAGATC GAGCAGTCCA TCGAAACCCT CAATATGTTG 
ATGCTGGACC TGGAGCCAGC CTCCGCAGCT GCCCCTCTTC ATAAGTCCCA GAGTGTCCCT 
GGGGCCTGGC CAGGGGCTTC TCCACTCTCT TCCCAGCCCC TCTTGGGGTC TTCCCGCCAG 
TCCCACCCAC TTACCCAGTC CCGATCTGGC TATATCCCCA GTGGGCATTC TCTGGGAACC 
CCGGAGCTGG TCTCTTCTGG CAGGCCTTAT TCACCTTACG ATTATCAGCT GCATCCAGCT 
GGATCCAACC AGAGTTTCCA TCCAAAAAGT CCAGCCTCTT CCACCTTCCT TCCAAGCCCT 
CATAGCTCTG CGGGGCCTCA GGAGCCCCCA GCCTCTCTCC CTGGTCTCAT CGCTCAGCCC 
CAGCTCCCAC CAAAGGAAAC TACATCAGAT CCCTCCCGAA CTCCAGAGGA AGAGCCGCTG 
AACCTGGAAG GGCTGGTAGC CCATCGGGTA GCAGTGTACA ATGCCAGACT ACAGAGCCTG 
AGGCAGAGCC GCGGCTTCCA ACCCTCTCCC CACTGGCGCC GATCTGCCTC CCTCTCTGGG 
GTTCAGGCTC GGGAGAGGCA GCCTGCAGAG CCCCCAGGCC CACTCCGGAG GCGGGCAGCC 
AGTGATGGAC AGTATGAAAA CCAGTCTCCG GAGGCCACAT CCCCCCGTAG TCCTGGGGTC 
CGCTCCCCCG TCCAGTGCGT CTCCCCTGAG CTGGCTCTTA CCATTGCCCT CAATCCTGGA 
GGGCGCCCCA AAGAGCCTCA TTTGCATAGC TACAAGGAGG CCTTTGAGGA GATGGAGGGG 
ACCTCCCCAA GCAGCCCGCC ACACAGTGTG GCGCGCTCTC CTCCAGGTCT GGCCAAGACA 
CCCCTGTCTG CTCTGGGCCT GAAACCCCAC AACCCAGCAG ACATCTTGCT GCACCCCACA 
GGTGTTGCCA GAAGACTGAT CCAGCCAGAG GAAGACGAGG GGGAGGAGGT GACTAAGCCT 
CCAGAAGAGC CCCGGAGCTA TGTGGAGTCC GTGGCGAGGA CAGCGGTGGC AGGGCCTCGA 
GCTCAGGACG TTGAGCCTAA GAGCTTCAGC GCACCAGCTG CTCACGCCTA TGGGCACGAG 
ACACCCCTGA GGAACGGGAC CCCAGGGGGC TCCTTTGTCT CCCCGAGCCC CCTCTCCACA 
AGCAGCCCCA TCCTCAGTGC TGACAGCACT TCCGTGGGCA GTTTCCCATC AGTGGTGAGC 
AGTGACCAGG GTCCCCGGAC ACCCTTCCAG CCTATGCTGG ACTCCAGCAT CCGCTCAGGC 
AGCTTGGGGC AGCCGAGCCC TGCAGCACTG AGTTATCAGA GCTCCTCGCC TGTCCCTGTT 
GGTGGCAGCA GCTACAACAG CCCTGACTAC TCCCTCCAAC CATTCAGCTC TTCTCCAGAA 
AGCCAAGGCC AGCCACAGTA CAGTGCGGCC AGTGTCCACA TGGTACCTGG GAGCCCTCAG 
GCACGACACA GGACAGTGGG TACCAACACT CCACCTAGCC CTGGTTTTGG CCGGCGGGCC 
GTCAACCCCA CCATGGCTGC CCCTGGTAGT CCCAGTTTGA GCCATCGGCA GGTGATGGGT 
CCATCTGGTC CAGGTTTCCA TGGTAACGTG GTTTCTGGCC ACCCAGCCAG TGCAGCTACC 
ACTCCTGGAA GCCCCAGCCT GGGCCGGCAC CCAGTGGGAA GCCATCAAGT TCCAGGCCTC 
CACAGCAGTG TGGTCACCAC TCCGGGAAGC CCAAGCCTGG GCCGACACCC TGGTGCCCAC 
CAAGGGAATC TGGCCTCCAG TCTCCACAGC AATGCAGTCA TCAGCCCTGG GAGCCCAAGC 
TTGGGCCGGC ACCTGGGTGG GTCTGGATCC GTGGTTCCTG GAAGCCCCAG CTTGGACCGG 
CATGCAGCTT ATGGTGGCTA CTCTACCCCT GAGGACCGAA GACCAACACT GTCCCGGCAA 
AGCAGTGCCT CTGGCTACCA GGCCCCATCC ACACCGTCAT TCCCTGTCTC TCCTGCCTAC 
TACCCTGGCC TGAGCAGCCC TGCCACCTCA CCCTCGCCAG ACTCAGCAGC CTTCCGGCAG 
GGGAGCCCCA CACCAGCCTT ACCAGAGAAG CGAAGAATGT CAGTGGGAGA CCGGGCAGGC 
AGCCTCCCCA ACTATGCCAC CATCAATGGG AAAGTGTCTT CCTCGCCTGT CGCCAATGGC 
ATGGCCAGTG GGAGCAGCAC CGTCTCCTTC TCTCACACGC TTCCGGACTT TTCTAAGTAT 
TCGATGCCAG ACAATAGTCC GGAGACTCGG GCTAAAGTGA AGTTTGTCCA GGACACTTCC 
AAGTATTGGT ACAAGCCTGA GATCTCCAGA GAACAGGCCA TCGCACTCCT AAAGGACCAG 
GAGCCAGGAG CCTTCATCAT CCGGGACAGC CACTCCTTCC GAGGGGCTTA TGGGCTGGCC 
ATGAAGGTGT CCTCACCCCC TCCAACCATC ACTCAGCAGG GCAAGAAAGG AGACATGACC 
CATGAGCTGG TGAGGCACTT TCTGATCGAG ACAGGCCCTA GAGGAGTGAA GCTCAAGGGT 
TGTCCCAATG AGCCAAACTT TGGTTCCCTT TCTGCCCTGG TCTATCAGCA CTCCGTCATC 
CCACTGGCCC TGCCCTGTAA ACTGGTCATT CCAAGCCGAG ACCCCACAGA TGAATCAAAA 
GATAGCTCAG GCCCTGCCAA CTCAACCACT GACCTGCTGA AGCAAGGAGC AGCTTGCAAT 
GTGCTCTTCG TCAACTCTGT GGATATGGAG TCACTCACCG GGCCACAGGC CATTTCCAAA 
GCCACATCTG AGACTCTGGC TGCCGACCCC ACACCGGCTG CCACCATTGT TCACTTCAAG 
GTCTCTGCCC AAGGAATCAC ACTGACTGAC AATCAAAGAA AGCTCTTCTT TAGAAGACAC 
TACCCCCTCA ACACTGTCAC CTTCTGTGAC CTGGATCCAC AAGAAAGAAA GTGGATGAAG 
ACAGAGGGAG GCGCCCCTGC TAAGCTCTTT GGCTTCGTGG CCCGGAAGCA GGGCAGCACC 
ACAGACAACG CTTGCCACCT CTTCGCAGAG CTTGACCCCA ACCAGCCTGC GTCTGCCATC 
GTCAACTTCG TCTCCAAGGT CATGCTGAGT GCTGGCCAGA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108843.1
CDS	142..5769
Translation	MTWICLSCMLWPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEEVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_030252983.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252983.1

ORF Insert Sequence:

ATGACGTGGA TTTGTCTGTC CTGCATGCTC TGGCCAGAAG ACCTGGAGGC CCCCAAGACC 
CACCACTTCA AGGTGAAGGC CTTCAAGAAG GTGAAGCCCT GTGGCATCTG CCGGCAGGCC 
ATCACTCGGG AAGGCTGTGT TTGCAAAGTC TGCAGCTTCT CCTGTCATCG GAAATGCCAG 
GCCAAGGTGG CTGCCCCTTG CGTTCCTCCG TCCAGCCATG AACTGGTGCC CATCACCACA 
GAGACCGTAC CCAAGAATGT AGTAGATGTG GGGGAAGGAG ACTGCCGGGT TGGAAGTTCT 
CCGAAAAACC TGGAGGAGGG CGGCTCCATG AGAGTTTCTC CAAGCATACA GCCACAACCA 
CAATCACAGC CTACCAGTCT CTCTAGAAAC ACGAGTGTGA GCCGAGCCAT GGAAGACAGT 
TGTGAGCTGG ACCTGGTGTA TGTCACCGAA CGCATCATCG CCGTGTCCTT CCCTAGCACA 
GCCAATGAGG AGAACTTCCG CAGCAACCTC CGTGAAGTGG CTCAGATGTT GAAGTCCAAG 
CATGGGGGCA ACTACCTGCT ATTCAACCTC TCTGAACAGA GGCCTGACAT CACCAAGCTG 
CATGCTAAGG TCCTGGAATT CGGCTGGCCT GACCTGCACA CACCGGCCCT GGAGAAGATA 
TGCAGTGTCT GTAAGGCCAT GGACACCTGG CTCAATGCAG ACCCCCACAA TGTCGTTGTT 
CTCCACAACA AGGGAAACCG AGGCAGGATT GGGGTTGTCA TTGCAGCGTA TCTGCACTAC 
AGCAACATTT CAGCCAGTGC TGACCAGGCT CTGGACCGGT TTGCAATGAA GAGATTCTAT 
GAGGACAAGA TCGTGCCCAT TGGCCAGCCA TCCCAGAGGA GATATGTGCA CTACTTCAGT 
GGCCTACTCT CTGGCTCCAT CAAAATGAAC AACAAGCCTT TGTTTCTGCA TCATGTGATC 
ATGCACGGCA TCCCCAACTT TGAGTCTAAA GGAGGGTGCC GGCCCTTTCT CCGCATCTAC 
CAAGCCATGC AACCTGTGTA CACATCTGGC ATTTACAACA TCCCTGGAGA CAGCCAGGCC 
AGCATCTGTA TCACCATTGA ACCAGGGCTG CTCTTGAAGG GAGACATCTT GCTGAAGTGT 
TATCATAAGA AATTCCGGAG CCCGGCTCGA GACGTCATCT TCCGAGTGCA GTTCCACACC 
TGTGCCATCC ATGACTTGGG GGTTGTTTTT GGGAAGGAAG ATCTGGATGA GGCCTTCAAA 
GATGATCGAT TTCCTGACTA TGGCAAAGTG GAGTTTGTCT TTTCCTATGG ACCAGAGAAA 
ATTCAAGGCA TGGAACACCT GGAGAATGGG CCCAGCGTAT CTGTAGACTA TAACACTTCT 
GACCCCCTCA TCCGCTGGGA CTCCTATGAC AACTTCAGTG GGCATCGTGA GGATGGCATG 
GAAGAAGTGG TGGGACACAC CCAAGGGCCG CTGGATGGGA GCCTGTACGC CAAGGTGAAG 
AAGAAGGATT CCCTGAATGG CAGTTCGGGG CCTGTCACTA CTGCACGGCC TGCCCTGTCA 
GCCACTCCCA ACCACGTGGA ACACACCCTG TCTGTGAGCA GCGACTCTGG CAACTCCACA 
GCCTCCACTA AGACAGACAA GACCGATGAG CCAGTCTCTG GGGCTACCAC TGCCCCTGCT 
GCCCTGAGTC CTCAGGAGAA GAAAGAGCTG GACCGCCTAC TAAGTGGCTT TGGTGTGGAT 
AGAGAAAAGC AAGGCGCCAT GTACCGGGCT CAGCAACTCA GATCCCATCC AGGCGGGGGC 
CCGACTGTGC CCTCCCCTGG CCGCCACATT GTCCCAGCCC AGGTTCACGT CAATGGTGGG 
GCCCTGGCAT CTGAGCGGGA GACAGACATT CTAGATGATG AGCTGCCCAT CCAGGACGGG 
CAGAGTGGGG GCAGCATGGG AACGCTGTCC TCCCTGGATG GGGTCACCAA CACCAGTGAA 
AGTGGCTACC CAGAGACCCT GTCCCCACTG ACCAATGGCC TGGACAAACC CTATTCCACA 
GAACCTGTGC TCAACGGCGG AGGCTACCCC TATGAGGCTG CCAACCGGGT GATTCCTGTC 
CACAGCAGTC ACTCGGCCCC CATTCGACCC TCCTACTCTG CTCAGGAGGG TTTAGCTGGC 
TACCAGCGTG AGGGACCCCA CCCAGCCTGG TCACAGCAAG TGACCAGTGC CCACTGTGGC 
TGTGACCCCA GTGGTCTATT CAGATCCCAG TCCTTTCCAG ATGTGGAGCC CCAGCTGCCC 
CAAGCTCCAA CCCGTGGGGG TAGTAGCCGA GAGGCTGTGC AGAGGGGTCT GAATTCATGG 
CAACAGCAGC AGCCCCACCC ACCTCCCCGC CAGCAGGAGA GATCCCCACT GCAGAGTCTT 
GCCCGCAGCA AGCCTAGCCC CCAGCTGTCA GCAGAGACCC CCGTAGCTGC TCTCCCAGAG 
TTCCCAAGGG CAGCGTCCCA GCAGGAGATC GAGCAGTCCA TCGAAACCCT CAATATGTTG 
ATGCTGGACC TGGAGCCAGC CTCCGCAGCT GCCCCTCTTC ATAAGTCCCA GAGTGTCCCT 
GGGGCCTGGC CAGGGGCTTC TCCACTCTCT TCCCAGCCCC TCTTGGGGTC TTCCCGCCAG 
TCCCACCCAC TTACCCAGTC CCGATCTGGC TATATCCCCA GTGGGCATTC TCTGGGAACC 
CCGGAGCTGG TCTCTTCTGG CAGGCCTTAT TCACCTTACG ATTATCAGCT GCATCCAGCT 
GGATCCAACC AGAGTTTCCA TCCAAAAAGT CCAGCCTCTT CCACCTTCCT TCCAAGCCCT 
CATAGCTCTG CGGGGCCTCA GGAGCCCCCA GCCTCTCTCC CTGGTCTCAT CGCTCAGCCC 
CAGCTCCCAC CAAAGGAAAC TACATCAGAT CCCTCCCGAA CTCCAGAGGA AGAGCCGCTG 
AACCTGGAAG GGCTGGTAGC CCATCGGGTA GCAGTGTACA ATGCCAGACT ACAGAGCCTG 
AGGCAGAGCC GCGGCTTCCA ACCCTCTCCC CACTGGCGCC GATCTGCCTC CCTCTCTGGG 
GTTCAGGCTC GGGAGAGGCA GCCTGCAGAG CCCCCAGGCC CACTCCGGAG GCGGGCAGCC 
AGTGATGGAC AGTATGAAAA CCAGTCTCCG GAGGCCACAT CCCCCCGTAG TCCTGGGGTC 
CGCTCCCCCG TCCAGTGCGT CTCCCCTGAG CTGGCTCTTA CCATTGCCCT CAATCCTGGA 
GGGCGCCCCA AAGAGCCTCA TTTGCATAGC TACAAGGAGG CCTTTGAGGA GATGGAGGGG 
ACCTCCCCAA GCAGCCCGCC ACACAGTGTG GCGCGCTCTC CTCCAGGTCT GGCCAAGACA 
CCCCTGTCTG CTCTGGGCCT GAAACCCCAC AACCCAGCAG ACATCTTGCT GCACCCCACA 
GGTGTTGCCA GAAGACTGAT CCAGCCAGAG GAAGACGAGG GGGAGGAGGT GACTAAGCCT 
CCAGAAGAGC CCCGGAGCTA TGTGGAGTCC GTGGCGAGGA CAGCGGTGGC AGGGCCTCGA 
GCTCAGGACG TTGAGCCTAA GAGCTTCAGC GCACCAGCTG CTCACGCCTA TGGGCACGAG 
ACACCCCTGA GGAACGGGAC CCCAGGGGGC TCCTTTGTCT CCCCGAGCCC CCTCTCCACA 
AGCAGCCCCA TCCTCAGTGC TGACAGCACT TCCGTGGGCA GTTTCCCATC AGTGGTGAGC 
AGTGACCAGG GTCCCCGGAC ACCCTTCCAG CCTATGCTGG ACTCCAGCAT CCGCTCAGGC 
AGCTTGGGGC AGCCGAGCCC TGCAGCACTG AGTTATCAGA GCTCCTCGCC TGTCCCTGTT 
GGTGGCAGCA GCTACAACAG CCCTGACTAC TCCCTCCAAC CATTCAGCTC TTCTCCAGAA 
AGCCAAGGCC AGCCACAGTA CAGTGCGGCC AGTGTCCACA TGGTACCTGG GAGCCCTCAG 
GCACGACACA GGACAGTGGG TACCAACACT CCACCTAGCC CTGGTTTTGG CCGGCGGGCC 
GTCAACCCCA CCATGGCTGC CCCTGGTAGT CCCAGTTTGA GCCATCGGCA GGTGATGGGT 
CCATCTGGTC CAGGTTTCCA TGGTAACGTG GTTTCTGGCC ACCCAGCCAG TGCAGCTACC 
ACTCCTGGAA GCCCCAGCCT GGGCCGGCAC CCAGTGGGAA GCCATCAAGT TCCAGGCCTC 
CACAGCAGTG TGGTCACCAC TCCGGGAAGC CCAAGCCTGG GCCGACACCC TGGTGCCCAC 
CAAGGGAATC TGGCCTCCAG TCTCCACAGC AATGCAGTCA TCAGCCCTGG GAGCCCAAGC 
TTGGGCCGGC ACCTGGGTGG GTCTGGATCC GTGGTTCCTG GAAGCCCCAG CTTGGACCGG 
CATGCAGCTT ATGGTGGCTA CTCTACCCCT GAGGACCGAA GACCAACACT GTCCCGGCAA 
AGCAGTGCCT CTGGCTACCA GGCCCCATCC ACACCGTCAT TCCCTGTCTC TCCTGCCTAC 
TACCCTGGCC TGAGCAGCCC TGCCACCTCA CCCTCGCCAG ACTCAGCAGC CTTCCGGCAG 
GGGAGCCCCA CACCAGCCTT ACCAGAGAAG CGAAGAATGT CAGTGGGAGA CCGGGCAGGC 
AGCCTCCCCA ACTATGCCAC CATCAATGGG AAAGTGTCTT CCTCGCCTGT CGCCAATGGC 
ATGGCCAGTG GGAGCAGCAC CGTCTCCTTC TCTCACACGC TTCCGGACTT TTCTAAGTAT 
TCGATGCCAG ACAATAGTCC GGAGACTCGG GCTAAAGTGA AGTTTGTCCA GGACACTTCC 
AAGTATTGGT ACAAGCCTGA GATCTCCAGA GAACAGGCCA TCGCACTCCT AAAGGACCAG 
GAGCCAGGAG CCTTCATCAT CCGGGACAGC CACTCCTTCC GAGGGGCTTA TGGGCTGGCC 
ATGAAGGTGT CCTCACCCCC TCCAACCATC ACTCAGCAGG GCAAGAAAGG AGACATGACC 
CATGAGCTGG TGAGGCACTT TCTGATCGAG ACAGGCCCTA GAGGAGTGAA GCTCAAGGGT 
TGTCCCAATG AGCCAAACTT TGGTTCCCTT TCTGCCCTGG TCTATCAGCA CTCCGTCATC 
CCACTGGCCC TGCCCTGTAA ACTGGTCATT CCAAGCCGAG ACCCCACAGA TGAATCAAAA 
GATAGCTCAG GCCCTGCCAA CTCAACCACT GACCTGCTGA AGCAAGGAGC AGCTTGCAAT 
GTGCTCTTCG TCAACTCTGT GGATATGGAG TCACTCACCG GGCCACAGGC CATTTCCAAA 
GCCACATCTG AGACTCTGGC TGCCGACCCC ACACCGGCTG CCACCATTGT TCACTTCAAG 
GTCTCTGCCC AAGGAATCAC ACTGACTGAC AATCAAAGAA AGCTCTTCTT TAGAAGACAC 
TACCCCCTCA ACACTGTCAC CTTCTGTGAC CTGGATCCAC AAGAAAGAAA GTGGATGAAG 
ACAGAGGGAG GCGCCCCTGC TAAGCTCTTT GGCTTCGTGG CCCGGAAGCA GGGCAGCACC 
ACAGACAACG CTTGCCACCT CTTCGCAGAG CTTGACCCCA ACCAGCCTGC GTCTGCCATC 
GTCAACTTCG TCTCCAAGGT CATGCTGAGT GCTGGCCAGA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_030252984.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108844.1 Mus musculus tensin 1 (Tns1), transcript variant X20, mRNA.

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CloneID	OMu85106
Clone ID Related Accession (Same CDS sequence)	XM_030252984.1
Accession Version	XM_030252984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGCGCGC 
TCTCCTCCAG GTCTGGCCAA GACACCCCTG TCTGCTCTGG GCCTGAAACC CCACAACCCA 
GCAGACATCT TGCTGCACCC CACAGGTGAG CCCCGGAGCT ATGTGGAGTC CGTGGCGAGG 
ACAGCGGTGG CAGGGCCTCG AGCTCAGGAC GTTGAGCCTA AGAGCTTCAG CGCACCAGCT 
GCTCACGCCT ATGGGCACGA GACACCCCTG AGGAACGGGA CCCCAGGGGG CTCCTTTGTC 
TCCCCGAGCC CCCTCTCCAC AAGCAGCCCC ATCCTCAGTG CTGACAGCAC TTCCGTGGGC 
AGTTTCCCAT CAGTGGTGAG CAGTGACCAG GGTCCCCGGA CACCCTTCCA GCCTATGCTG 
GACTCCAGCA TCCGCTCAGG CAGCTTGGGG CAGCCGAGCC CTGCAGCACT GAGTTATCAG 
AGCTCCTCGC CTGTCCCTGT TGGTGGCAGC AGCTACAACA GCCCTGACTA CTCCCTCCAA 
CCATTCAGCT CTTCTCCAGA AAGCCAAGGC CAGCCACAGT ACAGTGCGGC CAGTGTCCAC 
ATGGTACCTG GGAGCCCTCA GGCACGACAC AGGACAGTGG GTACCAACAC TCCACCTAGC 
CCTGGTTTTG GCCGGCGGGC CGTCAACCCC ACCATGGCTG CCCCTGGTAG TCCCAGTTTG 
AGCCATCGGC AGGTGATGGG TCCATCTGGT CCAGGTTTCC ATGGTAACGT GGTTTCTGGC 
CACCCAGCCA GTGCAGCTAC CACTCCTGGA AGCCCCAGCC TGGGCCGGCA CCCAGTGGGA 
AGCCATCAAG TTCCAGGCCT CCACAGCAGT GTGGTCACCA CTCCGGGAAG CCCAAGCCTG 
GGCCGACACC CTGGTGCCCA CCAAGGGAAT CTGGCCTCCA GTCTCCACAG CAATGCAGTC 
ATCAGCCCTG GGAGCCCAAG CTTGGGCCGG CACCTGGGTG GGTCTGGATC CGTGGTTCCT 
GGAAGCCCCA GCTTGGACCG GCATGCAGCT TATGGTGGCT ACTCTACCCC TGAGGACCGA 
AGACCAACAC TGTCCCGGCA AAGCAGTGCC TCTGGCTACC AGGCCCCATC CACACCGTCA 
TTCCCTGTCT CTCCTGCCTA CTACCCTGGC CTGAGCAGCC CTGCCACCTC ACCCTCGCCA 
GACTCAGCAG CCTTCCGGCA GGGGAGCCCC ACACCAGCCT TACCAGAGAA GCGAAGAATG 
TCAGTGGGAG ACCGGGCAGG CAGCCTCCCC AACTATGCCA CCATCAATGG GAAAGTGTCT 
TCCTCGCCTG TCGCCAATGG CATGGCCAGT GGGAGCAGCA CCGTCTCCTT CTCTCACACG 
CTTCCGGACT TTTCTAAGTA TTCGATGCCA GACAATAGTC CGGAGACTCG GGCTAAAGTG 
AAGTTTGTCC AGGACACTTC CAAGTATTGG TACAAGCCTG AGATCTCCAG AGAACAGGCC 
ATCGCACTCC TAAAGGACCA GGAGCCAGGA GCCTTCATCA TCCGGGACAG CCACTCCTTC 
CGAGGGGCTT ATGGGCTGGC CATGAAGGTG TCCTCACCCC CTCCAACCAT CACTCAGCAG 
GGCAAGAAAG GAGACATGAC CCATGAGCTG GTGAGGCACT TTCTGATCGA GACAGGCCCT 
AGAGGAGTGA AGCTCAAGGG TTGTCCCAAT GAGCCAAACT TTGGTTCCCT TTCTGCCCTG 
GTCTATCAGC ACTCCGTCAT CCCACTGGCC CTGCCCTGTA AACTGGTCAT TCCAAGCCGA 
GACCCCACAG ATGAATCAAA AGATAGCTCA GGCCCTGCCA ACTCAACCAC TGACCTGCTG 
AAGCAAGGAG CAGCTTGCAA TGTGCTCTTC GTCAACTCTG TGGATATGGA GTCACTCACC 
GGGCCACAGG CCATTTCCAA AGCCACATCT GAGACTCTGG CTGCCGACCC CACACCGGCT 
GCCACCATTG TTCACTTCAA GGTCTCTGCC CAAGGAATCA CACTGACTGA CAATCAAAGA 
AAGCTCTTCT TTAGAAGACA CTACCCCCTC AACACTGTCA CCTTCTGTGA CCTGGATCCA 
CAAGAAAGAA AGTGGATGAA GACAGAGGGA GGCGCCCCTG CTAAGCTCTT TGGCTTCGTG 
GCCCGGAAGC AGGGCAGCAC CACAGACAAC GCTTGCCACC TCTTCGCAGA GCTTGACCCC 
AACCAGCCTG CGTCTGCCAT CGTCAACTTC GTCTCCAAGG TCATGCTGAG TGCTGGCCAG 
AAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108844.1
CDS	16..5364
Translation	MLGDWGKGRGQPTGSPCPPWEHILFWNLGVSLPPCCSRESGVPWTQGALRRKLLVLPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAARSPPGLAKTPLSALGLKPHNPADILLHPTGEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_030252984.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252984.1

ORF Insert Sequence:

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGCGCGC 
TCTCCTCCAG GTCTGGCCAA GACACCCCTG TCTGCTCTGG GCCTGAAACC CCACAACCCA 
GCAGACATCT TGCTGCACCC CACAGGTGAG CCCCGGAGCT ATGTGGAGTC CGTGGCGAGG 
ACAGCGGTGG CAGGGCCTCG AGCTCAGGAC GTTGAGCCTA AGAGCTTCAG CGCACCAGCT 
GCTCACGCCT ATGGGCACGA GACACCCCTG AGGAACGGGA CCCCAGGGGG CTCCTTTGTC 
TCCCCGAGCC CCCTCTCCAC AAGCAGCCCC ATCCTCAGTG CTGACAGCAC TTCCGTGGGC 
AGTTTCCCAT CAGTGGTGAG CAGTGACCAG GGTCCCCGGA CACCCTTCCA GCCTATGCTG 
GACTCCAGCA TCCGCTCAGG CAGCTTGGGG CAGCCGAGCC CTGCAGCACT GAGTTATCAG 
AGCTCCTCGC CTGTCCCTGT TGGTGGCAGC AGCTACAACA GCCCTGACTA CTCCCTCCAA 
CCATTCAGCT CTTCTCCAGA AAGCCAAGGC CAGCCACAGT ACAGTGCGGC CAGTGTCCAC 
ATGGTACCTG GGAGCCCTCA GGCACGACAC AGGACAGTGG GTACCAACAC TCCACCTAGC 
CCTGGTTTTG GCCGGCGGGC CGTCAACCCC ACCATGGCTG CCCCTGGTAG TCCCAGTTTG 
AGCCATCGGC AGGTGATGGG TCCATCTGGT CCAGGTTTCC ATGGTAACGT GGTTTCTGGC 
CACCCAGCCA GTGCAGCTAC CACTCCTGGA AGCCCCAGCC TGGGCCGGCA CCCAGTGGGA 
AGCCATCAAG TTCCAGGCCT CCACAGCAGT GTGGTCACCA CTCCGGGAAG CCCAAGCCTG 
GGCCGACACC CTGGTGCCCA CCAAGGGAAT CTGGCCTCCA GTCTCCACAG CAATGCAGTC 
ATCAGCCCTG GGAGCCCAAG CTTGGGCCGG CACCTGGGTG GGTCTGGATC CGTGGTTCCT 
GGAAGCCCCA GCTTGGACCG GCATGCAGCT TATGGTGGCT ACTCTACCCC TGAGGACCGA 
AGACCAACAC TGTCCCGGCA AAGCAGTGCC TCTGGCTACC AGGCCCCATC CACACCGTCA 
TTCCCTGTCT CTCCTGCCTA CTACCCTGGC CTGAGCAGCC CTGCCACCTC ACCCTCGCCA 
GACTCAGCAG CCTTCCGGCA GGGGAGCCCC ACACCAGCCT TACCAGAGAA GCGAAGAATG 
TCAGTGGGAG ACCGGGCAGG CAGCCTCCCC AACTATGCCA CCATCAATGG GAAAGTGTCT 
TCCTCGCCTG TCGCCAATGG CATGGCCAGT GGGAGCAGCA CCGTCTCCTT CTCTCACACG 
CTTCCGGACT TTTCTAAGTA TTCGATGCCA GACAATAGTC CGGAGACTCG GGCTAAAGTG 
AAGTTTGTCC AGGACACTTC CAAGTATTGG TACAAGCCTG AGATCTCCAG AGAACAGGCC 
ATCGCACTCC TAAAGGACCA GGAGCCAGGA GCCTTCATCA TCCGGGACAG CCACTCCTTC 
CGAGGGGCTT ATGGGCTGGC CATGAAGGTG TCCTCACCCC CTCCAACCAT CACTCAGCAG 
GGCAAGAAAG GAGACATGAC CCATGAGCTG GTGAGGCACT TTCTGATCGA GACAGGCCCT 
AGAGGAGTGA AGCTCAAGGG TTGTCCCAAT GAGCCAAACT TTGGTTCCCT TTCTGCCCTG 
GTCTATCAGC ACTCCGTCAT CCCACTGGCC CTGCCCTGTA AACTGGTCAT TCCAAGCCGA 
GACCCCACAG ATGAATCAAA AGATAGCTCA GGCCCTGCCA ACTCAACCAC TGACCTGCTG 
AAGCAAGGAG CAGCTTGCAA TGTGCTCTTC GTCAACTCTG TGGATATGGA GTCACTCACC 
GGGCCACAGG CCATTTCCAA AGCCACATCT GAGACTCTGG CTGCCGACCC CACACCGGCT 
GCCACCATTG TTCACTTCAA GGTCTCTGCC CAAGGAATCA CACTGACTGA CAATCAAAGA 
AAGCTCTTCT TTAGAAGACA CTACCCCCTC AACACTGTCA CCTTCTGTGA CCTGGATCCA 
CAAGAAAGAA AGTGGATGAA GACAGAGGGA GGCGCCCCTG CTAAGCTCTT TGGCTTCGTG 
GCCCGGAAGC AGGGCAGCAC CACAGACAAC GCTTGCCACC TCTTCGCAGA GCTTGACCCC 
AACCAGCCTG CGTCTGCCAT CGTCAACTTC GTCTCCAAGG TCATGCTGAG TGCTGGCCAG 
AAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_030252985.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108845.1 Mus musculus tensin 1 (Tns1), transcript variant X30, mRNA.

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CloneID	OMu85107
Clone ID Related Accession (Same CDS sequence)	XM_030252985.1
Accession Version	XM_030252985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGAAG TGGTGGGACA CACCCAAGGG 
CCGCTGGATG GGAGCCTGTA CGCCAAGGTG AAGAAGAAGG ATTCCCTGAA TGGCAGTTCG 
GGGCCTGTCA CTACTGCACG GCCTGCCCTG TCAGCCACTC CCAACCACGT GGAACACACC 
CTGTCTGTGA GCAGCGACTC TGGCAACTCC ACAGCCTCCA CTAAGACAGA CAAGACCGAT 
GAGCCAGTCT CTGGGGCTAC CACTGCCCCT GCTGCCCTGA GTCCTCAGGA GAAGAAAGAG 
CTGGACCGCC TACTAAGTGG CTTTGGTGTG GATAGAGAAA AGCAAGGCGC CATGTACCGG 
GCTCAGCAAC TCAGATCCCA TCCAGGCGGG GGCCCGACTG TGCCCTCCCC TGGCCGCCAC 
ATTGTCCCAG CCCAGGTTCA CGTCAATGGT GGGGCCCTGG CATCTGAGCG GGAGACAGAC 
ATTCTAGATG ATGAGCTGCC CATCCAGGAC GGGCAGAGTG GGGGCAGCAT GGGAACGCTG 
TCCTCCCTGG ATGGGGTCAC CAACACCAGT GAAAGTGGCT ACCCAGAGAC CCTGTCCCCA 
CTGACCAATG GCCTGGACAA ACCCTATTCC ACAGAACCTG TGCTCAACGG CGGAGGCTAC 
CCCTATGAGG CTGCCAACCG GGTGATTCCT GTCCACAGCA GTCACTCGGC CCCCATTCGA 
CCCTCCTACT CTGCTCAGGA GGGTTTAGCT GGCTACCAGC GTGAGGGACC CCACCCAGCC 
TGGTCACAGC AAGTGACCAG TGCCCACTGT GGCTGTGACC CCAGTGGTCT ATTCAGATCC 
CAGTCCTTTC CAGATGTGGA GCCCCAGCTG CCCCAAGCTC CAACCCGTGG GGGTAGTAGC 
CGAGAGGCTG TGCAGAGGGG TCTGAATTCA TGGCAACAGC AGCAGCCCCA CCCACCTCCC 
CGCCAGCAGG AGAGATCCCC ACTGCAGAGT CTTGCCCGCA GCAAGCCTAG CCCCCAGCTG 
TCAGCAGAGA CCCCCGTAGC TGCTCTCCCA GAGTTCCCAA GGGCAGCGTC CCAGCAGGAG 
ATCGAGCAGT CCATCGAAAC CCTCAATATG TTGATGCTGG ACCTGGAGCC AGCCTCCGCA 
GCTGCCCCTC TTCATAAGTC CCAGAGTGTC CCTGGGGCCT GGCCAGGGGC TTCTCCACTC 
TCTTCCCAGC CCCTCTTGGG GTCTTCCCGC CAGTCCCACC CACTTACCCA GTCCCGATCT 
GGCTATATCC CCAGTGGGCA TTCTCTGGGA ACCCCGGAGC TGGTCTCTTC TGGCAGGCCT 
TATTCACCTT ACGATTATCA GCTGCATCCA GCTGGATCCA ACCAGAGTTT CCATCCAAAA 
AGTCCAGCCT CTTCCACCTT CCTTCCAAGC CCTCATAGCT CTGCGGGGCC TCAGGAGCCC 
CCAGCCTCTC TCCCTGGTCT CATCGCTCAG CCCCAGCTCC CACCAAAGGA AACTACATCA 
GATCCCTCCC GAACTCCAGA GGAAGAGCCG CTGAACCTGG AAGGGCTGGT AGCCCATCGG 
GTAGCAGTGT ACAATGCCAG ACTACAGAGC CTGAGGCAGA GCCGCGGCTT CCAACCCTCT 
CCCCACTGGC GCCGATCTGC CTCCCTCTCT GGGGTTCAGG CTCGGGAGAG GCAGCCTGCA 
GAGCCCCCAG GCCCACTCCG GAGGCGGGCA GCCAGTGATG GACAGTATGA AAACCAGTCT 
CCGGAGGCCA CATCCCCCCG TAGTCCTGGG GTCCGCTCCC CCGTCCAGTG CGTCTCCCCT 
GAGCTGGCTC TTACCATTGC CCTCAATCCT GGAGGGCGCC CCAAAGAGCC TCATTTGCAT 
AGCTACAAGG AGGCCTTTGA GGAGATGGAG GGGACCTCCC CAAGCAGCCC GCCACACAGT 
GTGGCGCGCT CTCCTCCAGG TCTGGCCAAG ACACCCCTGT CTGCTCTGGG CCTGAAACCC 
CACAACCCAG CAGACATCTT GCTGCACCCC ACAGGTGTTG CCAGAAGACT GATCCAGCCA 
GAGGAAGACG AGGGGGAGGA GGTGACTAAG CCTCCAGAAG AGCCCCGGAG CTATGTGGAG 
TCCGTGGCGA GGACAGCGGT GGCAGGGCCT CGAGCTCAGG ACGTTGAGCC TAAGAGCTTC 
AGCGCACCAG CTGCTCACGC CTATGGGCAC GAGACACCCC TGAGGAACGG GACCCCAGGG 
GGCTCCTTTG TCTCCCCGAG CCCCCTCTCC ACAAGCAGCC CCATCCTCAG TGCTGACAGC 
ACTTCCGTGG GCAGTTTCCC ATCAGTGGTG AGCAGTGACC AGGGTCCCCG GACACCCTTC 
CAGCCTATGC TGGACTCCAG CATCCGCTCA GGCAGCTTGG GGCAGCCGAG CCCTGCAGCA 
CTGAGTTATC AGAGCTCCTC GCCTGTCCCT GTTGGTGGCA GCAGCTACAA CAGCCCTGAC 
TACTCCCTCC AACCATTCAG CTCTTCTCCA GAAAGCCAAG GCCAGCCACA GTACAGTGCG 
GCCAGTGTCC ACATGGTACC TGGGAGCCCT CAGGCACGAC ACAGGACAGT GGGTACCAAC 
ACTCCACCTA GCCCTGGTTT TGGCCGGCGG GCCGTCAACC CCACCATGGC TGCCCCTGGT 
AGTCCCAGTT TGAGCCATCG GCAGGTGATG GGTCCATCTG GTCCAGGTTT CCATGGTAAC 
GTGGTTTCTG GCCACCCAGC CAGTGCAGCT ACCACTCCTG GAAGCCCCAG CCTGGGCCGG 
CACCCAGTGG GAAGCCATCA AGTTCCAGGC CTCCACAGCA GTGTGGTCAC CACTCCGGGA 
AGCCCAAGCC TGGGCCGACA CCCTGGTGCC CACCAAGGGA ATCTGGCCTC CAGTCTCCAC 
AGCAATGCAG TCATCAGCCC TGGGAGCCCA AGCTTGGGCC GGCACCTGGG TGGGTCTGGA 
TCCGTGGTTC CTGGAAGCCC CAGCTTGGAC CGGCATGCAG CTTATGGTGG CTACTCTACC 
CCTGAGGACC GAAGACCAAC ACTGTCCCGG CAAAGCAGTG CCTCTGGCTA CCAGGCCCCA 
TCCACACCGT CATTCCCTGT CTCTCCTGCC TACTACCCTG GCCTGAGCAG CCCTGCCACC 
TCACCCTCGC CAGACTCAGC AGCCTTCCGG CAGGGGAGCC CCACACCAGC CTTACCAGAG 
AAGCGAAGAA TGTCAGTGGG AGACCGGGCA GGCAGCCTCC CCAACTATGC CACCATCAAT 
GGGAAAGTGT CTTCCTCGCC TGTCGCCAAT GGCATGGCCA GTGGGAGCAG CACCGTCTCC 
TTCTCTCACA CGCTTCCGGA CTTTTCTAAG TATTCGATGC CAGACAATAG TCCGGAGACT 
CGGGCTAAAG TGAAGTTTGT CCAGGACACT TCCAAGTATT GGTACAAGCC TGAGATCTCC 
AGAGAACAGG CCATCGCACT CCTAAAGGAC CAGGAGCCAG GAGCCTTCAT CATCCGGGAC 
AGCCACTCCT TCCGAGGGGC TTATGGGCTG GCCATGAAGG TGTCCTCACC CCCTCCAACC 
ATCACTCAGC AGGGCAAGAA AGGAGACATG ACCCATGAGC TGGTGAGGCA CTTTCTGATC 
GAGACAGGCC CTAGAGGAGT GAAGCTCAAG GGTTGTCCCA ATGAGCCAAA CTTTGGTTCC 
CTTTCTGCCC TGGTCTATCA GCACTCCGTC ATCCCACTGG CCCTGCCCTG TAAACTGGTC 
ATTCCAAGCC GAGACCCCAC AGATGAATCA AAAGATAGCT CAGGCCCTGC CAACTCAACC 
ACTGACCTGC TGAAGCAAGG AGCAGCTTGC AATGTGCTCT TCGTCAACTC TGTGGATATG 
GAGTCACTCA CCGGGCCACA GGCCATTTCC AAAGCCACAT CTGAGACTCT GGCTGCCGAC 
CCCACACCGG CTGCCACCAT TGTTCACTTC AAGGTCTCTG CCCAAGGAAT CACACTGACT 
GACAATCAAA GAAAGCTCTT CTTTAGAAGA CACTACCCCC TCAACACTGT CACCTTCTGT 
GACCTGGATC CACAAGAAAG AAAGTGGATG AAGACAGAGG GAGGCGCCCC TGCTAAGCTC 
TTTGGCTTCG TGGCCCGGAA GCAGGGCAGC ACCACAGACA ACGCTTGCCA CCTCTTCGCA 
GAGCTTGACC CCAACCAGCC TGCGTCTGCC ATCGTCAACT TCGTCTCCAA GGTCATGCTG 
AGTGCTGGCC AGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108845.1
CDS	355..5655
Translation	MRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEEVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_030252985.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252985.1

ORF Insert Sequence:

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGAAG TGGTGGGACA CACCCAAGGG 
CCGCTGGATG GGAGCCTGTA CGCCAAGGTG AAGAAGAAGG ATTCCCTGAA TGGCAGTTCG 
GGGCCTGTCA CTACTGCACG GCCTGCCCTG TCAGCCACTC CCAACCACGT GGAACACACC 
CTGTCTGTGA GCAGCGACTC TGGCAACTCC ACAGCCTCCA CTAAGACAGA CAAGACCGAT 
GAGCCAGTCT CTGGGGCTAC CACTGCCCCT GCTGCCCTGA GTCCTCAGGA GAAGAAAGAG 
CTGGACCGCC TACTAAGTGG CTTTGGTGTG GATAGAGAAA AGCAAGGCGC CATGTACCGG 
GCTCAGCAAC TCAGATCCCA TCCAGGCGGG GGCCCGACTG TGCCCTCCCC TGGCCGCCAC 
ATTGTCCCAG CCCAGGTTCA CGTCAATGGT GGGGCCCTGG CATCTGAGCG GGAGACAGAC 
ATTCTAGATG ATGAGCTGCC CATCCAGGAC GGGCAGAGTG GGGGCAGCAT GGGAACGCTG 
TCCTCCCTGG ATGGGGTCAC CAACACCAGT GAAAGTGGCT ACCCAGAGAC CCTGTCCCCA 
CTGACCAATG GCCTGGACAA ACCCTATTCC ACAGAACCTG TGCTCAACGG CGGAGGCTAC 
CCCTATGAGG CTGCCAACCG GGTGATTCCT GTCCACAGCA GTCACTCGGC CCCCATTCGA 
CCCTCCTACT CTGCTCAGGA GGGTTTAGCT GGCTACCAGC GTGAGGGACC CCACCCAGCC 
TGGTCACAGC AAGTGACCAG TGCCCACTGT GGCTGTGACC CCAGTGGTCT ATTCAGATCC 
CAGTCCTTTC CAGATGTGGA GCCCCAGCTG CCCCAAGCTC CAACCCGTGG GGGTAGTAGC 
CGAGAGGCTG TGCAGAGGGG TCTGAATTCA TGGCAACAGC AGCAGCCCCA CCCACCTCCC 
CGCCAGCAGG AGAGATCCCC ACTGCAGAGT CTTGCCCGCA GCAAGCCTAG CCCCCAGCTG 
TCAGCAGAGA CCCCCGTAGC TGCTCTCCCA GAGTTCCCAA GGGCAGCGTC CCAGCAGGAG 
ATCGAGCAGT CCATCGAAAC CCTCAATATG TTGATGCTGG ACCTGGAGCC AGCCTCCGCA 
GCTGCCCCTC TTCATAAGTC CCAGAGTGTC CCTGGGGCCT GGCCAGGGGC TTCTCCACTC 
TCTTCCCAGC CCCTCTTGGG GTCTTCCCGC CAGTCCCACC CACTTACCCA GTCCCGATCT 
GGCTATATCC CCAGTGGGCA TTCTCTGGGA ACCCCGGAGC TGGTCTCTTC TGGCAGGCCT 
TATTCACCTT ACGATTATCA GCTGCATCCA GCTGGATCCA ACCAGAGTTT CCATCCAAAA 
AGTCCAGCCT CTTCCACCTT CCTTCCAAGC CCTCATAGCT CTGCGGGGCC TCAGGAGCCC 
CCAGCCTCTC TCCCTGGTCT CATCGCTCAG CCCCAGCTCC CACCAAAGGA AACTACATCA 
GATCCCTCCC GAACTCCAGA GGAAGAGCCG CTGAACCTGG AAGGGCTGGT AGCCCATCGG 
GTAGCAGTGT ACAATGCCAG ACTACAGAGC CTGAGGCAGA GCCGCGGCTT CCAACCCTCT 
CCCCACTGGC GCCGATCTGC CTCCCTCTCT GGGGTTCAGG CTCGGGAGAG GCAGCCTGCA 
GAGCCCCCAG GCCCACTCCG GAGGCGGGCA GCCAGTGATG GACAGTATGA AAACCAGTCT 
CCGGAGGCCA CATCCCCCCG TAGTCCTGGG GTCCGCTCCC CCGTCCAGTG CGTCTCCCCT 
GAGCTGGCTC TTACCATTGC CCTCAATCCT GGAGGGCGCC CCAAAGAGCC TCATTTGCAT 
AGCTACAAGG AGGCCTTTGA GGAGATGGAG GGGACCTCCC CAAGCAGCCC GCCACACAGT 
GTGGCGCGCT CTCCTCCAGG TCTGGCCAAG ACACCCCTGT CTGCTCTGGG CCTGAAACCC 
CACAACCCAG CAGACATCTT GCTGCACCCC ACAGGTGTTG CCAGAAGACT GATCCAGCCA 
GAGGAAGACG AGGGGGAGGA GGTGACTAAG CCTCCAGAAG AGCCCCGGAG CTATGTGGAG 
TCCGTGGCGA GGACAGCGGT GGCAGGGCCT CGAGCTCAGG ACGTTGAGCC TAAGAGCTTC 
AGCGCACCAG CTGCTCACGC CTATGGGCAC GAGACACCCC TGAGGAACGG GACCCCAGGG 
GGCTCCTTTG TCTCCCCGAG CCCCCTCTCC ACAAGCAGCC CCATCCTCAG TGCTGACAGC 
ACTTCCGTGG GCAGTTTCCC ATCAGTGGTG AGCAGTGACC AGGGTCCCCG GACACCCTTC 
CAGCCTATGC TGGACTCCAG CATCCGCTCA GGCAGCTTGG GGCAGCCGAG CCCTGCAGCA 
CTGAGTTATC AGAGCTCCTC GCCTGTCCCT GTTGGTGGCA GCAGCTACAA CAGCCCTGAC 
TACTCCCTCC AACCATTCAG CTCTTCTCCA GAAAGCCAAG GCCAGCCACA GTACAGTGCG 
GCCAGTGTCC ACATGGTACC TGGGAGCCCT CAGGCACGAC ACAGGACAGT GGGTACCAAC 
ACTCCACCTA GCCCTGGTTT TGGCCGGCGG GCCGTCAACC CCACCATGGC TGCCCCTGGT 
AGTCCCAGTT TGAGCCATCG GCAGGTGATG GGTCCATCTG GTCCAGGTTT CCATGGTAAC 
GTGGTTTCTG GCCACCCAGC CAGTGCAGCT ACCACTCCTG GAAGCCCCAG CCTGGGCCGG 
CACCCAGTGG GAAGCCATCA AGTTCCAGGC CTCCACAGCA GTGTGGTCAC CACTCCGGGA 
AGCCCAAGCC TGGGCCGACA CCCTGGTGCC CACCAAGGGA ATCTGGCCTC CAGTCTCCAC 
AGCAATGCAG TCATCAGCCC TGGGAGCCCA AGCTTGGGCC GGCACCTGGG TGGGTCTGGA 
TCCGTGGTTC CTGGAAGCCC CAGCTTGGAC CGGCATGCAG CTTATGGTGG CTACTCTACC 
CCTGAGGACC GAAGACCAAC ACTGTCCCGG CAAAGCAGTG CCTCTGGCTA CCAGGCCCCA 
TCCACACCGT CATTCCCTGT CTCTCCTGCC TACTACCCTG GCCTGAGCAG CCCTGCCACC 
TCACCCTCGC CAGACTCAGC AGCCTTCCGG CAGGGGAGCC CCACACCAGC CTTACCAGAG 
AAGCGAAGAA TGTCAGTGGG AGACCGGGCA GGCAGCCTCC CCAACTATGC CACCATCAAT 
GGGAAAGTGT CTTCCTCGCC TGTCGCCAAT GGCATGGCCA GTGGGAGCAG CACCGTCTCC 
TTCTCTCACA CGCTTCCGGA CTTTTCTAAG TATTCGATGC CAGACAATAG TCCGGAGACT 
CGGGCTAAAG TGAAGTTTGT CCAGGACACT TCCAAGTATT GGTACAAGCC TGAGATCTCC 
AGAGAACAGG CCATCGCACT CCTAAAGGAC CAGGAGCCAG GAGCCTTCAT CATCCGGGAC 
AGCCACTCCT TCCGAGGGGC TTATGGGCTG GCCATGAAGG TGTCCTCACC CCCTCCAACC 
ATCACTCAGC AGGGCAAGAA AGGAGACATG ACCCATGAGC TGGTGAGGCA CTTTCTGATC 
GAGACAGGCC CTAGAGGAGT GAAGCTCAAG GGTTGTCCCA ATGAGCCAAA CTTTGGTTCC 
CTTTCTGCCC TGGTCTATCA GCACTCCGTC ATCCCACTGG CCCTGCCCTG TAAACTGGTC 
ATTCCAAGCC GAGACCCCAC AGATGAATCA AAAGATAGCT CAGGCCCTGC CAACTCAACC 
ACTGACCTGC TGAAGCAAGG AGCAGCTTGC AATGTGCTCT TCGTCAACTC TGTGGATATG 
GAGTCACTCA CCGGGCCACA GGCCATTTCC AAAGCCACAT CTGAGACTCT GGCTGCCGAC 
CCCACACCGG CTGCCACCAT TGTTCACTTC AAGGTCTCTG CCCAAGGAAT CACACTGACT 
GACAATCAAA GAAAGCTCTT CTTTAGAAGA CACTACCCCC TCAACACTGT CACCTTCTGT 
GACCTGGATC CACAAGAAAG AAAGTGGATG AAGACAGAGG GAGGCGCCCC TGCTAAGCTC 
TTTGGCTTCG TGGCCCGGAA GCAGGGCAGC ACCACAGACA ACGCTTGCCA CCTCTTCGCA 
GAGCTTGACC CCAACCAGCC TGCGTCTGCC ATCGTCAACT TCGTCTCCAA GGTCATGCTG 
AGTGCTGGCC AGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495872.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495935.1 Mus musculus tensin 1 (Tns1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OMu27360
Clone ID Related Accession (Same CDS sequence)	XM_006495872.1
Accession Version	XM_006495872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTGGA TTTGTCTGTC CTGCATGCTC TGGCCAGAAG ACCTGGAGGC CCCCAAGACC 
CACCACTTCA AGGTGAAGGC CTTCAAGAAG GTGAAGCCCT GTGGCATCTG CCGGCAGGCC 
ATCACTCGGG AAGGCTGTGT TTGCAAAGTC TGCAGCTTCT CCTGTCATCG GAAATGCCAG 
GCCAAGGTGG CTGCCCCTTG CGTTCCTCCG TCCAGCCATG AACTGGTGCC CATCACCACA 
GAGACCGTAC CCAAGAATGT AGTAGATGTG GGGGAAGGAG ACTGCCGGGT TGGAAGTTCT 
CCGAAAAACC TGGAGGAGGG CGGCTCCATG AGAGTTTCTC CAAGCATACA GCCACAACCA 
CAATCACAGC CTACCAGTCT CTCTAGAAAC ACGAGTGTGA GCCGAGCCAT GGAAGACAGT 
TGTGAGCTGG ACCTGGTGTA TGTCACCGAA CGCATCATCG CCGTGTCCTT CCCTAGCACA 
GCCAATGAGG AGAACTTCCG CAGCAACCTC CGTGAAGTGG CTCAGATGTT GAAGTCCAAG 
CATGGGGGCA ACTACCTGCT ATTCAACCTC TCTGAACAGA GGCCTGACAT CACCAAGCTG 
CATGCTAAGG TCCTGGAATT CGGCTGGCCT GACCTGCACA CACCGGCCCT GGAGAAGATA 
TGCAGTGTCT GTAAGGCCAT GGACACCTGG CTCAATGCAG ACCCCCACAA TGTCGTTGTT 
CTCCACAACA AGGGAAACCG AGGCAGGATT GGGGTTGTCA TTGCAGCGTA TCTGCACTAC 
AGCAACATTT CAGCCAGTGC TGACCAGGCT CTGGACCGGT TTGCAATGAA GAGATTCTAT 
GAGGACAAGA TCGTGCCCAT TGGCCAGCCA TCCCAGAGGA GATATGTGCA CTACTTCAGT 
GGCCTACTCT CTGGCTCCAT CAAAATGAAC AACAAGCCTT TGTTTCTGCA TCATGTGATC 
ATGCACGGCA TCCCCAACTT TGAGTCTAAA GGAGGGTGCC GGCCCTTTCT CCGCATCTAC 
CAAGCCATGC AACCTGTGTA CACATCTGGC ATTTACAACA TCCCTGGAGA CAGCCAGGCC 
AGCATCTGTA TCACCATTGA ACCAGGGCTG CTCTTGAAGG GAGACATCTT GCTGAAGTGT 
TATCATAAGA AATTCCGGAG CCCGGCTCGA GACGTCATCT TCCGAGTGCA GTTCCACACC 
TGTGCCATCC ATGACTTGGG GGTTGTTTTT GGGAAGGAAG ATCTGGATGA GGCCTTCAAA 
GATGATCGAT TTCCTGACTA TGGCAAAGTG GAGTTTGTCT TTTCCTATGG ACCAGAGAAA 
ATTCAAGGCA TGGAACACCT GGAGAATGGG CCCAGCGTAT CTGTAGACTA TAACACTTCT 
GACCCCCTCA TCCGCTGGGA CTCCTATGAC AACTTCAGTG GGCATCGTGA GGATGGCATG 
GAAGGTTTAA AAGTGGTGGG ACACACCCAA GGGCCGCTGG ATGGGAGCCT GTACGCCAAG 
GTGAAGAAGA AGGATTCCCT GAATGGCAGT TCGGGGCCTG TCACTACTGC ACGGCCTGCC 
CTGTCAGCCA CTCCCAACCA CGTGGAACAC ACCCTGTCTG TGAGCAGCGA CTCTGGCAAC 
TCCACAGCCT CCACTAAGAC AGACAAGACC GATGAGCCAG TCTCTGGGGC TACCACTGCC 
CCTGCTGCCC TGAGTCCTCA GGAGAAGAAA GAGCTGGACC GCCTACTAAG TGGCTTTGGT 
GTGGATAGAG AAAAGCAAGG CGCCATGTAC CGGGCTCAGC AACTCAGATC CCATCCAGGC 
GGGGGCCCGA CTGTGCCCTC CCCTGGCCGC CACATTGTCC CAGCCCAGGT TCACGTCAAT 
GGTGGGGCCC TGGCATCTGA GCGGGAGACA GACATTCTAG ATGATGAGCT GCCCATCCAG 
GACGGGCAGA GTGGGGGCAG CATGGGAACG CTGTCCTCCC TGGATGGGGT CACCAACACC 
AGTGAAAGTG GCTACCCAGA GACCCTGTCC CCACTGACCA ATGGCCTGGA CAAACCCTAT 
TCCACAGAAC CTGTGCTCAA CGGCGGAGGC TACCCCTATG AGGCTGCCAA CCGGGTGATT 
CCTGTCCACA GCAGTCACTC GGCCCCCATT CGACCCTCCT ACTCTGCTCA GGAGGGTTTA 
GCTGGCTACC AGCGTGAGGG ACCCCACCCA GCCTGGTCAC AGCAAGTGAC CAGTGCCCAC 
TGTGGCTGTG ACCCCAGTGG TCTATTCAGA TCCCAGTCCT TTCCAGATGT GGAGCCCCAG 
CTGCCCCAAG CTCCAACCCG TGGGGGTAGT AGCCGAGAGG CTGTGCAGAG GGGTCTGAAT 
TCATGGCAAC AGCAGCAGCC CCACCCACCT CCCCGCCAGC AGGAGAGATC CCCACTGCAG 
AGTCTTGCCC GCAGCAAGCC TAGCCCCCAG CTGTCAGCAG AGACCCCCGT AGCTGCTCTC 
CCAGAGTTCC CAAGGGCAGC GTCCCAGCAG GAGATCGAGC AGTCCATCGA AACCCTCAAT 
ATGTTGATGC TGGACCTGGA GCCAGCCTCC GCAGCTGCCC CTCTTCATAA GTCCCAGAGT 
GTCCCTGGGG CCTGGCCAGG GGCTTCTCCA CTCTCTTCCC AGCCCCTCTT GGGGTCTTCC 
CGCCAGTCCC ACCCACTTAC CCAGTCCCGA TCTGGCTATA TCCCCAGTGG GCATTCTCTG 
GGAACCCCGG AGCTGGTCTC TTCTGGCAGG CCTTATTCAC CTTACGATTA TCAGCTGCAT 
CCAGCTGGAT CCAACCAGAG TTTCCATCCA AAAAGTCCAG CCTCTTCCAC CTTCCTTCCA 
AGCCCTCATA GCTCTGCGGG GCCTCAGGAG CCCCCAGCCT CTCTCCCTGG TCTCATCGCT 
CAGCCCCAGC TCCCACCAAA GGAAACTACA TCAGATCCCT CCCGAACTCC AGAGGAAGAG 
CCGCTGAACC TGGAAGGGCT GGTAGCCCAT CGGGTAGCAG TGTACAATGC CAGACTACAG 
AGCCTGAGGC AGAGCCGCGG CTTCCAACCC TCTCCCCACT GGCGCCGATC TGCCTCCCTC 
TCTGGGGTTC AGGCTCGGGA GAGGCAGCCT GCAGAGCCCC CAGGCCCACT CCGGAGGCGG 
GCAGCCAGTG ATGGACAGTA TGAAAACCAG TCTCCGGAGG CCACATCCCC CCGTAGTCCT 
GGGGTCCGCT CCCCCGTCCA GTGCGTCTCC CCTGAGCTGG CTCTTACCAT TGCCCTCAAT 
CCTGGAGGGC GCCCCAAAGA GCCTCATTTG CATAGCTACA AGGAGGCCTT TGAGGAGATG 
GAGGGGACCT CCCCAAGCAG CCCGCCACAC AGTGTGGCGC GCTCTCCTCC AGGTCTGGCC 
AAGACACCCC TGTCTGCTCT GGGCCTGAAA CCCCACAACC CAGCAGACAT CTTGCTGCAC 
CCCACAGGTG TTGCCAGAAG ACTGATCCAG CCAGAGGAAG ACGAGGGGGA GGAGGTGACT 
AAGCCTCCAG AAGAGCCCCG GAGCTATGTG GAGTCCGTGG CGAGGACAGC GGTGGCAGGG 
CCTCGAGCTC AGGACGTTGA GCCTAAGAGC TTCAGCGCAC CAGCTGCTCA CGCCTATGGG 
CACGAGACAC CCCTGAGGAA CGGGACCCCA GGGGGCTCCT TTGTCTCCCC GAGCCCCCTC 
TCCACAAGCA GCCCCATCCT CAGTGCTGAC AGCACTTCCG TGGGCAGTTT CCCATCAGTG 
GTGAGCAGTG ACCAGGGTCC CCGGACACCC TTCCAGCCTA TGCTGGACTC CAGCATCCGC 
TCAGGCAGCT TGGGGCAGCC GAGCCCTGCA GCACTGAGTT ATCAGAGCTC CTCGCCTGTC 
CCTGTTGGTG GCAGCAGCTA CAACAGCCCT GACTACTCCC TCCAACCATT CAGCTCTTCT 
CCAGAAAGCC AAGGCCAGCC ACAGTACAGT GCGGCCAGTG TCCACATGGT ACCTGGGAGC 
CCTCAGGCAC GACACAGGAC AGTGGGTACC AACACTCCAC CTAGCCCTGG TTTTGGCCGG 
CGGGCCGTCA ACCCCACCAT GGCTGCCCCT GGTAGTCCCA GTTTGAGCCA TCGGCAGGTG 
ATGGGTCCAT CTGGTCCAGG TTTCCATGGT AACGTGGTTT CTGGCCACCC AGCCAGTGCA 
GCTACCACTC CTGGAAGCCC CAGCCTGGGC CGGCACCCAG TGGGAAGCCA TCAAGTTCCA 
GGCCTCCACA GCAGTGTGGT CACCACTCCG GGAAGCCCAA GCCTGGGCCG ACACCCTGGT 
GCCCACCAAG GGAATCTGGC CTCCAGTCTC CACAGCAATG CAGTCATCAG CCCTGGGAGC 
CCAAGCTTGG GCCGGCACCT GGGTGGGTCT GGATCCGTGG TTCCTGGAAG CCCCAGCTTG 
GACCGGCATG CAGCTTATGG TGGCTACTCT ACCCCTGAGG ACCGAAGACC AACACTGTCC 
CGGCAAAGCA GTGCCTCTGG CTACCAGGCC CCATCCACAC CGTCATTCCC TGTCTCTCCT 
GCCTACTACC CTGGCCTGAG CAGCCCTGCC ACCTCACCCT CGCCAGACTC AGCAGCCTTC 
CGGCAGGGGA GCCCCACACC AGCCTTACCA GAGAAGCGAA GAATGTCAGT GGGAGACCGG 
GCAGGCAGCC TCCCCAACTA TGCCACCATC AATGGGAAAG TGTCTTCCTC GCCTGTCGCC 
AATGGCATGG CCAGTGGGAG CAGCACCGTC TCCTTCTCTC ACACGCTTCC GGACTTTTCT 
AAGTATTCGA TGCCAGACAA TAGTCCGGAG ACTCGGGCTA AAGTGAAGTT TGTCCAGGAC 
ACTTCCAAGT ATTGGTACAA GCCTGAGATC TCCAGAGAAC AGGCCATCGC ACTCCTAAAG 
GACCAGGAGC CAGGAGCCTT CATCATCCGG GACAGCCACT CCTTCCGAGG GGCTTATGGG 
CTGGCCATGA AGGTGTCCTC ACCCCCTCCA ACCATCACTC AGCAGGGCAA GAAAGGAGAC 
ATGACCCATG AGCTGGTGAG GCACTTTCTG ATCGAGACAG GCCCTAGAGG AGTGAAGCTC 
AAGGGTTGTC CCAATGAGCC AAACTTTGGT TCCCTTTCTG CCCTGGTCTA TCAGCACTCC 
GTCATCCCAC TGGCCCTGCC CTGTAAACTG GTCATTCCAA GCCGAGACCC CACAGATGAA 
TCAAAAGATA GCTCAGGCCC TGCCAACTCA ACCACTGACC TGCTGAAGCA AGGAGCAGCT 
TGCAATGTGC TCTTCGTCAA CTCTGTGGAT ATGGAGTCAC TCACCGGGCC ACAGGCCATT 
TCCAAAGCCA CATCTGAGAC TCTGGCTGCC GACCCCACAC CGGCTGCCAC CATTGTTCAC 
TTCAAGGTCT CTGCCCAAGG AATCACACTG ACTGACAATC AAAGAAAGCT CTTCTTTAGA 
AGACACTACC CCCTCAACAC TGTCACCTTC TGTGACCTGG ATCCACAAGA AAGAAAGTGG 
ATGAAGACAG AGGGAGGCGC CCCTGCTAAG CTCTTTGGCT TCGTGGCCCG GAAGCAGGGC 
AGCACCACAG ACAACGCTTG CCACCTCTTC GCAGAGCTTG ACCCCAACCA GCCTGCGTCT 
GCCATCGTCA ACTTCGTCTC CAAGGTCATG CTGAGTGCTG GCCAGAAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495935.1
CDS	142..5775
Translation	MTWICLSCMLWPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495872.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495872.1

ORF Insert Sequence:

ATGACGTGGA TTTGTCTGTC CTGCATGCTC TGGCCAGAAG ACCTGGAGGC CCCCAAGACC 
CACCACTTCA AGGTGAAGGC CTTCAAGAAG GTGAAGCCCT GTGGCATCTG CCGGCAGGCC 
ATCACTCGGG AAGGCTGTGT TTGCAAAGTC TGCAGCTTCT CCTGTCATCG GAAATGCCAG 
GCCAAGGTGG CTGCCCCTTG CGTTCCTCCG TCCAGCCATG AACTGGTGCC CATCACCACA 
GAGACCGTAC CCAAGAATGT AGTAGATGTG GGGGAAGGAG ACTGCCGGGT TGGAAGTTCT 
CCGAAAAACC TGGAGGAGGG CGGCTCCATG AGAGTTTCTC CAAGCATACA GCCACAACCA 
CAATCACAGC CTACCAGTCT CTCTAGAAAC ACGAGTGTGA GCCGAGCCAT GGAAGACAGT 
TGTGAGCTGG ACCTGGTGTA TGTCACCGAA CGCATCATCG CCGTGTCCTT CCCTAGCACA 
GCCAATGAGG AGAACTTCCG CAGCAACCTC CGTGAAGTGG CTCAGATGTT GAAGTCCAAG 
CATGGGGGCA ACTACCTGCT ATTCAACCTC TCTGAACAGA GGCCTGACAT CACCAAGCTG 
CATGCTAAGG TCCTGGAATT CGGCTGGCCT GACCTGCACA CACCGGCCCT GGAGAAGATA 
TGCAGTGTCT GTAAGGCCAT GGACACCTGG CTCAATGCAG ACCCCCACAA TGTCGTTGTT 
CTCCACAACA AGGGAAACCG AGGCAGGATT GGGGTTGTCA TTGCAGCGTA TCTGCACTAC 
AGCAACATTT CAGCCAGTGC TGACCAGGCT CTGGACCGGT TTGCAATGAA GAGATTCTAT 
GAGGACAAGA TCGTGCCCAT TGGCCAGCCA TCCCAGAGGA GATATGTGCA CTACTTCAGT 
GGCCTACTCT CTGGCTCCAT CAAAATGAAC AACAAGCCTT TGTTTCTGCA TCATGTGATC 
ATGCACGGCA TCCCCAACTT TGAGTCTAAA GGAGGGTGCC GGCCCTTTCT CCGCATCTAC 
CAAGCCATGC AACCTGTGTA CACATCTGGC ATTTACAACA TCCCTGGAGA CAGCCAGGCC 
AGCATCTGTA TCACCATTGA ACCAGGGCTG CTCTTGAAGG GAGACATCTT GCTGAAGTGT 
TATCATAAGA AATTCCGGAG CCCGGCTCGA GACGTCATCT TCCGAGTGCA GTTCCACACC 
TGTGCCATCC ATGACTTGGG GGTTGTTTTT GGGAAGGAAG ATCTGGATGA GGCCTTCAAA 
GATGATCGAT TTCCTGACTA TGGCAAAGTG GAGTTTGTCT TTTCCTATGG ACCAGAGAAA 
ATTCAAGGCA TGGAACACCT GGAGAATGGG CCCAGCGTAT CTGTAGACTA TAACACTTCT 
GACCCCCTCA TCCGCTGGGA CTCCTATGAC AACTTCAGTG GGCATCGTGA GGATGGCATG 
GAAGGTTTAA AAGTGGTGGG ACACACCCAA GGGCCGCTGG ATGGGAGCCT GTACGCCAAG 
GTGAAGAAGA AGGATTCCCT GAATGGCAGT TCGGGGCCTG TCACTACTGC ACGGCCTGCC 
CTGTCAGCCA CTCCCAACCA CGTGGAACAC ACCCTGTCTG TGAGCAGCGA CTCTGGCAAC 
TCCACAGCCT CCACTAAGAC AGACAAGACC GATGAGCCAG TCTCTGGGGC TACCACTGCC 
CCTGCTGCCC TGAGTCCTCA GGAGAAGAAA GAGCTGGACC GCCTACTAAG TGGCTTTGGT 
GTGGATAGAG AAAAGCAAGG CGCCATGTAC CGGGCTCAGC AACTCAGATC CCATCCAGGC 
GGGGGCCCGA CTGTGCCCTC CCCTGGCCGC CACATTGTCC CAGCCCAGGT TCACGTCAAT 
GGTGGGGCCC TGGCATCTGA GCGGGAGACA GACATTCTAG ATGATGAGCT GCCCATCCAG 
GACGGGCAGA GTGGGGGCAG CATGGGAACG CTGTCCTCCC TGGATGGGGT CACCAACACC 
AGTGAAAGTG GCTACCCAGA GACCCTGTCC CCACTGACCA ATGGCCTGGA CAAACCCTAT 
TCCACAGAAC CTGTGCTCAA CGGCGGAGGC TACCCCTATG AGGCTGCCAA CCGGGTGATT 
CCTGTCCACA GCAGTCACTC GGCCCCCATT CGACCCTCCT ACTCTGCTCA GGAGGGTTTA 
GCTGGCTACC AGCGTGAGGG ACCCCACCCA GCCTGGTCAC AGCAAGTGAC CAGTGCCCAC 
TGTGGCTGTG ACCCCAGTGG TCTATTCAGA TCCCAGTCCT TTCCAGATGT GGAGCCCCAG 
CTGCCCCAAG CTCCAACCCG TGGGGGTAGT AGCCGAGAGG CTGTGCAGAG GGGTCTGAAT 
TCATGGCAAC AGCAGCAGCC CCACCCACCT CCCCGCCAGC AGGAGAGATC CCCACTGCAG 
AGTCTTGCCC GCAGCAAGCC TAGCCCCCAG CTGTCAGCAG AGACCCCCGT AGCTGCTCTC 
CCAGAGTTCC CAAGGGCAGC GTCCCAGCAG GAGATCGAGC AGTCCATCGA AACCCTCAAT 
ATGTTGATGC TGGACCTGGA GCCAGCCTCC GCAGCTGCCC CTCTTCATAA GTCCCAGAGT 
GTCCCTGGGG CCTGGCCAGG GGCTTCTCCA CTCTCTTCCC AGCCCCTCTT GGGGTCTTCC 
CGCCAGTCCC ACCCACTTAC CCAGTCCCGA TCTGGCTATA TCCCCAGTGG GCATTCTCTG 
GGAACCCCGG AGCTGGTCTC TTCTGGCAGG CCTTATTCAC CTTACGATTA TCAGCTGCAT 
CCAGCTGGAT CCAACCAGAG TTTCCATCCA AAAAGTCCAG CCTCTTCCAC CTTCCTTCCA 
AGCCCTCATA GCTCTGCGGG GCCTCAGGAG CCCCCAGCCT CTCTCCCTGG TCTCATCGCT 
CAGCCCCAGC TCCCACCAAA GGAAACTACA TCAGATCCCT CCCGAACTCC AGAGGAAGAG 
CCGCTGAACC TGGAAGGGCT GGTAGCCCAT CGGGTAGCAG TGTACAATGC CAGACTACAG 
AGCCTGAGGC AGAGCCGCGG CTTCCAACCC TCTCCCCACT GGCGCCGATC TGCCTCCCTC 
TCTGGGGTTC AGGCTCGGGA GAGGCAGCCT GCAGAGCCCC CAGGCCCACT CCGGAGGCGG 
GCAGCCAGTG ATGGACAGTA TGAAAACCAG TCTCCGGAGG CCACATCCCC CCGTAGTCCT 
GGGGTCCGCT CCCCCGTCCA GTGCGTCTCC CCTGAGCTGG CTCTTACCAT TGCCCTCAAT 
CCTGGAGGGC GCCCCAAAGA GCCTCATTTG CATAGCTACA AGGAGGCCTT TGAGGAGATG 
GAGGGGACCT CCCCAAGCAG CCCGCCACAC AGTGTGGCGC GCTCTCCTCC AGGTCTGGCC 
AAGACACCCC TGTCTGCTCT GGGCCTGAAA CCCCACAACC CAGCAGACAT CTTGCTGCAC 
CCCACAGGTG TTGCCAGAAG ACTGATCCAG CCAGAGGAAG ACGAGGGGGA GGAGGTGACT 
AAGCCTCCAG AAGAGCCCCG GAGCTATGTG GAGTCCGTGG CGAGGACAGC GGTGGCAGGG 
CCTCGAGCTC AGGACGTTGA GCCTAAGAGC TTCAGCGCAC CAGCTGCTCA CGCCTATGGG 
CACGAGACAC CCCTGAGGAA CGGGACCCCA GGGGGCTCCT TTGTCTCCCC GAGCCCCCTC 
TCCACAAGCA GCCCCATCCT CAGTGCTGAC AGCACTTCCG TGGGCAGTTT CCCATCAGTG 
GTGAGCAGTG ACCAGGGTCC CCGGACACCC TTCCAGCCTA TGCTGGACTC CAGCATCCGC 
TCAGGCAGCT TGGGGCAGCC GAGCCCTGCA GCACTGAGTT ATCAGAGCTC CTCGCCTGTC 
CCTGTTGGTG GCAGCAGCTA CAACAGCCCT GACTACTCCC TCCAACCATT CAGCTCTTCT 
CCAGAAAGCC AAGGCCAGCC ACAGTACAGT GCGGCCAGTG TCCACATGGT ACCTGGGAGC 
CCTCAGGCAC GACACAGGAC AGTGGGTACC AACACTCCAC CTAGCCCTGG TTTTGGCCGG 
CGGGCCGTCA ACCCCACCAT GGCTGCCCCT GGTAGTCCCA GTTTGAGCCA TCGGCAGGTG 
ATGGGTCCAT CTGGTCCAGG TTTCCATGGT AACGTGGTTT CTGGCCACCC AGCCAGTGCA 
GCTACCACTC CTGGAAGCCC CAGCCTGGGC CGGCACCCAG TGGGAAGCCA TCAAGTTCCA 
GGCCTCCACA GCAGTGTGGT CACCACTCCG GGAAGCCCAA GCCTGGGCCG ACACCCTGGT 
GCCCACCAAG GGAATCTGGC CTCCAGTCTC CACAGCAATG CAGTCATCAG CCCTGGGAGC 
CCAAGCTTGG GCCGGCACCT GGGTGGGTCT GGATCCGTGG TTCCTGGAAG CCCCAGCTTG 
GACCGGCATG CAGCTTATGG TGGCTACTCT ACCCCTGAGG ACCGAAGACC AACACTGTCC 
CGGCAAAGCA GTGCCTCTGG CTACCAGGCC CCATCCACAC CGTCATTCCC TGTCTCTCCT 
GCCTACTACC CTGGCCTGAG CAGCCCTGCC ACCTCACCCT CGCCAGACTC AGCAGCCTTC 
CGGCAGGGGA GCCCCACACC AGCCTTACCA GAGAAGCGAA GAATGTCAGT GGGAGACCGG 
GCAGGCAGCC TCCCCAACTA TGCCACCATC AATGGGAAAG TGTCTTCCTC GCCTGTCGCC 
AATGGCATGG CCAGTGGGAG CAGCACCGTC TCCTTCTCTC ACACGCTTCC GGACTTTTCT 
AAGTATTCGA TGCCAGACAA TAGTCCGGAG ACTCGGGCTA AAGTGAAGTT TGTCCAGGAC 
ACTTCCAAGT ATTGGTACAA GCCTGAGATC TCCAGAGAAC AGGCCATCGC ACTCCTAAAG 
GACCAGGAGC CAGGAGCCTT CATCATCCGG GACAGCCACT CCTTCCGAGG GGCTTATGGG 
CTGGCCATGA AGGTGTCCTC ACCCCCTCCA ACCATCACTC AGCAGGGCAA GAAAGGAGAC 
ATGACCCATG AGCTGGTGAG GCACTTTCTG ATCGAGACAG GCCCTAGAGG AGTGAAGCTC 
AAGGGTTGTC CCAATGAGCC AAACTTTGGT TCCCTTTCTG CCCTGGTCTA TCAGCACTCC 
GTCATCCCAC TGGCCCTGCC CTGTAAACTG GTCATTCCAA GCCGAGACCC CACAGATGAA 
TCAAAAGATA GCTCAGGCCC TGCCAACTCA ACCACTGACC TGCTGAAGCA AGGAGCAGCT 
TGCAATGTGC TCTTCGTCAA CTCTGTGGAT ATGGAGTCAC TCACCGGGCC ACAGGCCATT 
TCCAAAGCCA CATCTGAGAC TCTGGCTGCC GACCCCACAC CGGCTGCCAC CATTGTTCAC 
TTCAAGGTCT CTGCCCAAGG AATCACACTG ACTGACAATC AAAGAAAGCT CTTCTTTAGA 
AGACACTACC CCCTCAACAC TGTCACCTTC TGTGACCTGG ATCCACAAGA AAGAAAGTGG 
ATGAAGACAG AGGGAGGCGC CCCTGCTAAG CTCTTTGGCT TCGTGGCCCG GAAGCAGGGC 
AGCACCACAG ACAACGCTTG CCACCTCTTC GCAGAGCTTG ACCCCAACCA GCCTGCGTCT 
GCCATCGTCA ACTTCGTCTC CAAGGTCATG CTGAGTGCTG GCCAGAAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495874.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495937.1 Mus musculus tensin 1 (Tns1), transcript variant X15, mRNA.

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CloneID	OMu27362
Clone ID Related Accession (Same CDS sequence)	XM_006495874.4 , XM_006495874.3
Accession Version	XM_006495874.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495874.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGT GCAAGGGTGC AGGGATACAG CCCAGCGGTC TGCATGCACT GATGTCTGGC 
CAGCCTCAGA TCCTGAACTT GGGGAAGGTG GCTGCCCCTT GCGTTCCTCC GTCCAGCCAT 
GAACTGGTGC CCATCACCAC AGAGACCGTA CCCAAGAATG TAGTAGATGT GGGGGAAGGA 
GACTGCCGGG TTGGAAGTTC TCCGAAAAAC CTGGAGGAGG GCGGCTCCAT GAGAGTTTCT 
CCAAGCATAC AGCCACAACC ACAATCACAG CCTACCAGTC TCTCTAGAAA CACGAGTGTG 
AGCCGAGCCA TGGAAGACAG TTGTGAGCTG GACCTGGTGT ATGTCACCGA ACGCATCATC 
GCCGTGTCCT TCCCTAGCAC AGCCAATGAG GAGAACTTCC GCAGCAACCT CCGTGAAGTG 
GCTCAGATGT TGAAGTCCAA GCATGGGGGC AACTACCTGC TATTCAACCT CTCTGAACAG 
AGGCCTGACA TCACCAAGCT GCATGCTAAG GTCCTGGAAT TCGGCTGGCC TGACCTGCAC 
ACACCGGCCC TGGAGAAGAT ATGCAGTGTC TGTAAGGCCA TGGACACCTG GCTCAATGCA 
GACCCCCACA ATGTCGTTGT TCTCCACAAC AAGGGAAACC GAGGCAGGAT TGGGGTTGTC 
ATTGCAGCGT ATCTGCACTA CAGCAACATT TCAGCCAGTG CTGACCAGGC TCTGGACCGG 
TTTGCAATGA AGAGATTCTA TGAGGACAAG ATCGTGCCCA TTGGCCAGCC ATCCCAGAGG 
AGATATGTGC ACTACTTCAG TGGCCTACTC TCTGGCTCCA TCAAAATGAA CAACAAGCCT 
TTGTTTCTGC ATCATGTGAT CATGCACGGC ATCCCCAACT TTGAGTCTAA AGGAGGGTGC 
CGGCCCTTTC TCCGCATCTA CCAAGCCATG CAACCTGTGT ACACATCTGG CATTTACAAC 
ATCCCTGGAG ACAGCCAGGC CAGCATCTGT ATCACCATTG AACCAGGGCT GCTCTTGAAG 
GGAGACATCT TGCTGAAGTG TTATCATAAG AAATTCCGGA GCCCGGCTCG AGACGTCATC 
TTCCGAGTGC AGTTCCACAC CTGTGCCATC CATGACTTGG GGGTTGTTTT TGGGAAGGAA 
GATCTGGATG AGGCCTTCAA AGATGATCGA TTTCCTGACT ATGGCAAAGT GGAGTTTGTC 
TTTTCCTATG GACCAGAGAA AATTCAAGGC ATGGAACACC TGGAGAATGG GCCCAGCGTA 
TCTGTAGACT ATAACACTTC TGACCCCCTC ATCCGCTGGG ACTCCTATGA CAACTTCAGT 
GGGCATCGTG AGGATGGCAT GGAAGGTTTA AAAGTGGTGG GACACACCCA AGGGCCGCTG 
GATGGGAGCC TGTACGCCAA GGTGAAGAAG AAGGATTCCC TGAATGGCAG TTCGGGGCCT 
GTCACTACTG CACGGCCTGC CCTGTCAGCC ACTCCCAACC ACGTGGAACA CACCCTGTCT 
GTGAGCAGCG ACTCTGGCAA CTCCACAGCC TCCACTAAGA CAGACAAGAC CGATGAGCCA 
GTCTCTGGGG CTACCACTGC CCCTGCTGCC CTGAGTCCTC AGGAGAAGAA AGAGCTGGAC 
CGCCTACTAA GTGGCTTTGG TGTGGATAGA GAAAAGCAAG GCGCCATGTA CCGGGCTCAG 
CAACTCAGAT CCCATCCAGG CGGGGGCCCG ACTGTGCCCT CCCCTGGCCG CCACATTGTC 
CCAGCCCAGG TTCACGTCAA TGGTGGGGCC CTGGCATCTG AGCGGGAGAC AGACATTCTA 
GATGATGAGC TGCCCATCCA GGACGGGCAG AGTGGGGGCA GCATGGGAAC GCTGTCCTCC 
CTGGATGGGG TCACCAACAC CAGTGAAAGT GGCTACCCAG AGACCCTGTC CCCACTGACC 
AATGGCCTGG ACAAACCCTA TTCCACAGAA CCTGTGCTCA ACGGCGGAGG CTACCCCTAT 
GAGGCTGCCA ACCGGGTGAT TCCTGTCCAC AGCAGTCACT CGGCCCCCAT TCGACCCTCC 
TACTCTGCTC AGGAGGGTTT AGCTGGCTAC CAGCGTGAGG GACCCCACCC AGCCTGGTCA 
CAGCAAGTGA CCAGTGCCCA CTGTGGCTGT GACCCCAGTG GTCTATTCAG ATCCCAGTCC 
TTTCCAGATG TGGAGCCCCA GCTGCCCCAA GCTCCAACCC GTGGGGGTAG TAGCCGAGAG 
GCTGTGCAGA GGGGTCTGAA TTCATGGCAA CAGCAGCAGC CCCACCCACC TCCCCGCCAG 
CAGGAGAGAT CCCCACTGCA GAGTCTTGCC CGCAGCAAGC CTAGCCCCCA GCTGTCAGCA 
GAGACCCCCG TAGCTGCTCT CCCAGAGTTC CCAAGGGCAG CGTCCCAGCA GGAGATCGAG 
CAGTCCATCG AAACCCTCAA TATGTTGATG CTGGACCTGG AGCCAGCCTC CGCAGCTGCC 
CCTCTTCATA AGTCCCAGAG TGTCCCTGGG GCCTGGCCAG GGGCTTCTCC ACTCTCTTCC 
CAGCCCCTCT TGGGGTCTTC CCGCCAGTCC CACCCACTTA CCCAGTCCCG ATCTGGCTAT 
ATCCCCAGTG GGCATTCTCT GGGAACCCCG GAGCTGGTCT CTTCTGGCAG GCCTTATTCA 
CCTTACGATT ATCAGCTGCA TCCAGCTGGA TCCAACCAGA GTTTCCATCC AAAAAGTCCA 
GCCTCTTCCA CCTTCCTTCC AAGCCCTCAT AGCTCTGCGG GGCCTCAGGA GCCCCCAGCC 
TCTCTCCCTG GTCTCATCGC TCAGCCCCAG CTCCCACCAA AGGAAACTAC ATCAGATCCC 
TCCCGAACTC CAGAGGAAGA GCCGCTGAAC CTGGAAGGGC TGGTAGCCCA TCGGGTAGCA 
GTGTACAATG CCAGACTACA GAGCCTGAGG CAGAGCCGCG GCTTCCAACC CTCTCCCCAC 
TGGCGCCGAT CTGCCTCCCT CTCTGGGGTT CAGGCTCGGG AGAGGCAGCC TGCAGAGCCC 
CCAGGCCCAC TCCGGAGGCG GGCAGCCAGT GATGGACAGT ATGAAAACCA GTCTCCGGAG 
GCCACATCCC CCCGTAGTCC TGGGGTCCGC TCCCCCGTCC AGTGCGTCTC CCCTGAGCTG 
GCTCTTACCA TTGCCCTCAA TCCTGGAGGG CGCCCCAAAG AGCCTCATTT GCATAGCTAC 
AAGGAGGCCT TTGAGGAGAT GGAGGGGACC TCCCCAAGCA GCCCGCCACA CAGTGTGGCG 
CGCTCTCCTC CAGGTCTGGC CAAGACACCC CTGTCTGCTC TGGGCCTGAA ACCCCACAAC 
CCAGCAGACA TCTTGCTGCA CCCCACAGGT GTTGCCAGAA GACTGATCCA GCCAGAGGAA 
GACGAGGGGG AGGAGGTGAC TAAGCCTCCA GAAGAGCCCC GGAGCTATGT GGAGTCCGTG 
GCGAGGACAG CGGTGGCAGG GCCTCGAGCT CAGGACGTTG AGCCTAAGAG CTTCAGCGCA 
CCAGCTGCTC ACGCCTATGG GCACGAGACA CCCCTGAGGA ACGGGACCCC AGGGGGCTCC 
TTTGTCTCCC CGAGCCCCCT CTCCACAAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCC 
GTGGGCAGTT TCCCATCAGT GGTGAGCAGT GACCAGGGTC CCCGGACACC CTTCCAGCCT 
ATGCTGGACT CCAGCATCCG CTCAGGCAGC TTGGGGCAGC CGAGCCCTGC AGCACTGAGT 
TATCAGAGCT CCTCGCCTGT CCCTGTTGGT GGCAGCAGCT ACAACAGCCC TGACTACTCC 
CTCCAACCAT TCAGCTCTTC TCCAGAAAGC CAAGGCCAGC CACAGTACAG TGCGGCCAGT 
GTCCACATGG TACCTGGGAG CCCTCAGGCA CGACACAGGA CAGTGGGTAC CAACACTCCA 
CCTAGCCCTG GTTTTGGCCG GCGGGCCGTC AACCCCACCA TGGCTGCCCC TGGTAGTCCC 
AGTTTGAGCC ATCGGCAGGT GATGGGTCCA TCTGGTCCAG GTTTCCATGG TAACGTGGTT 
TCTGGCCACC CAGCCAGTGC AGCTACCACT CCTGGAAGCC CCAGCCTGGG CCGGCACCCA 
GTGGGAAGCC ATCAAGTTCC AGGCCTCCAC AGCAGTGTGG TCACCACTCC GGGAAGCCCA 
AGCCTGGGCC GACACCCTGG TGCCCACCAA GGGAATCTGG CCTCCAGTCT CCACAGCAAT 
GCAGTCATCA GCCCTGGGAG CCCAAGCTTG GGCCGGCACC TGGGTGGGTC TGGATCCGTG 
GTTCCTGGAA GCCCCAGCTT GGACCGGCAT GCAGCTTATG GTGGCTACTC TACCCCTGAG 
GACCGAAGAC CAACACTGTC CCGGCAAAGC AGTGCCTCTG GCTACCAGGC CCCATCCACA 
CCGTCATTCC CTGTCTCTCC TGCCTACTAC CCTGGCCTGA GCAGCCCTGC CACCTCACCC 
TCGCCAGACT CAGCAGCCTT CCGGCAGGGG AGCCCCACAC CAGCCTTACC AGAGAAGCGA 
AGAATGTCAG TGGGAGACCG GGCAGGCAGC CTCCCCAACT ATGCCACCAT CAATGGGAAA 
GTGTCTTCCT CGCCTGTCGC CAATGGCATG GCCAGTGGGA GCAGCACCGT CTCCTTCTCT 
CACACGCTTC CGGACTTTTC TAAGTATTCG ATGCCAGACA ATAGTCCGGA GACTCGGGCT 
AAAGTGAAGT TTGTCCAGGA CACTTCCAAG TATTGGTACA AGCCTGAGAT CTCCAGAGAA 
CAGGCCATCG CACTCCTAAA GGACCAGGAG CCAGGAGCCT TCATCATCCG GGACAGCCAC 
TCCTTCCGAG GGGCTTATGG GCTGGCCATG AAGGTGTCCT CACCCCCTCC AACCATCACT 
CAGCAGGGCA AGAAAGGAGA CATGACCCAT GAGCTGGTGA GGCACTTTCT GATCGAGACA 
GGCCCTAGAG GAGTGAAGCT CAAGGGTTGT CCCAATGAGC CAAACTTTGG TTCCCTTTCT 
GCCCTGGTCT ATCAGCACTC CGTCATCCCA CTGGCCCTGC CCTGTAAACT GGTCATTCCA 
AGCCGAGACC CCACAGATGA ATCAAAAGAT AGCTCAGGCC CTGCCAACTC AACCACTGAC 
CTGCTGAAGC AAGGAGCAGC TTGCAATGTG CTCTTCGTCA ACTCTGTGGA TATGGAGTCA 
CTCACCGGGC CACAGGCCAT TTCCAAAGCC ACATCTGAGA CTCTGGCTGC CGACCCCACA 
CCGGCTGCCA CCATTGTTCA CTTCAAGGTC TCTGCCCAAG GAATCACACT GACTGACAAT 
CAAAGAAAGC TCTTCTTTAG AAGACACTAC CCCCTCAACA CTGTCACCTT CTGTGACCTG 
GATCCACAAG AAAGAAAGTG GATGAAGACA GAGGGAGGCG CCCCTGCTAA GCTCTTTGGC 
TTCGTGGCCC GGAAGCAGGG CAGCACCACA GACAACGCTT GCCACCTCTT CGCAGAGCTT 
GACCCCAACC AGCCTGCGTC TGCCATCGTC AACTTCGTCT CCAAGGTCAT GCTGAGTGCT 
GGCCAGAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495937.1
CDS	245..5779
Translation	MNVCKGAGIQPSGLHALMSGQPQILNLGKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495874.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495874.3

ORF Insert Sequence:

ATGAATGTGT GCAAGGGTGC AGGGATACAG CCCAGCGGTC TGCATGCACT GATGTCTGGC 
CAGCCTCAGA TCCTGAACTT GGGGAAGGTG GCTGCCCCTT GCGTTCCTCC GTCCAGCCAT 
GAACTGGTGC CCATCACCAC AGAGACCGTA CCCAAGAATG TAGTAGATGT GGGGGAAGGA 
GACTGCCGGG TTGGAAGTTC TCCGAAAAAC CTGGAGGAGG GCGGCTCCAT GAGAGTTTCT 
CCAAGCATAC AGCCACAACC ACAATCACAG CCTACCAGTC TCTCTAGAAA CACGAGTGTG 
AGCCGAGCCA TGGAAGACAG TTGTGAGCTG GACCTGGTGT ATGTCACCGA ACGCATCATC 
GCCGTGTCCT TCCCTAGCAC AGCCAATGAG GAGAACTTCC GCAGCAACCT CCGTGAAGTG 
GCTCAGATGT TGAAGTCCAA GCATGGGGGC AACTACCTGC TATTCAACCT CTCTGAACAG 
AGGCCTGACA TCACCAAGCT GCATGCTAAG GTCCTGGAAT TCGGCTGGCC TGACCTGCAC 
ACACCGGCCC TGGAGAAGAT ATGCAGTGTC TGTAAGGCCA TGGACACCTG GCTCAATGCA 
GACCCCCACA ATGTCGTTGT TCTCCACAAC AAGGGAAACC GAGGCAGGAT TGGGGTTGTC 
ATTGCAGCGT ATCTGCACTA CAGCAACATT TCAGCCAGTG CTGACCAGGC TCTGGACCGG 
TTTGCAATGA AGAGATTCTA TGAGGACAAG ATCGTGCCCA TTGGCCAGCC ATCCCAGAGG 
AGATATGTGC ACTACTTCAG TGGCCTACTC TCTGGCTCCA TCAAAATGAA CAACAAGCCT 
TTGTTTCTGC ATCATGTGAT CATGCACGGC ATCCCCAACT TTGAGTCTAA AGGAGGGTGC 
CGGCCCTTTC TCCGCATCTA CCAAGCCATG CAACCTGTGT ACACATCTGG CATTTACAAC 
ATCCCTGGAG ACAGCCAGGC CAGCATCTGT ATCACCATTG AACCAGGGCT GCTCTTGAAG 
GGAGACATCT TGCTGAAGTG TTATCATAAG AAATTCCGGA GCCCGGCTCG AGACGTCATC 
TTCCGAGTGC AGTTCCACAC CTGTGCCATC CATGACTTGG GGGTTGTTTT TGGGAAGGAA 
GATCTGGATG AGGCCTTCAA AGATGATCGA TTTCCTGACT ATGGCAAAGT GGAGTTTGTC 
TTTTCCTATG GACCAGAGAA AATTCAAGGC ATGGAACACC TGGAGAATGG GCCCAGCGTA 
TCTGTAGACT ATAACACTTC TGACCCCCTC ATCCGCTGGG ACTCCTATGA CAACTTCAGT 
GGGCATCGTG AGGATGGCAT GGAAGGTTTA AAAGTGGTGG GACACACCCA AGGGCCGCTG 
GATGGGAGCC TGTACGCCAA GGTGAAGAAG AAGGATTCCC TGAATGGCAG TTCGGGGCCT 
GTCACTACTG CACGGCCTGC CCTGTCAGCC ACTCCCAACC ACGTGGAACA CACCCTGTCT 
GTGAGCAGCG ACTCTGGCAA CTCCACAGCC TCCACTAAGA CAGACAAGAC CGATGAGCCA 
GTCTCTGGGG CTACCACTGC CCCTGCTGCC CTGAGTCCTC AGGAGAAGAA AGAGCTGGAC 
CGCCTACTAA GTGGCTTTGG TGTGGATAGA GAAAAGCAAG GCGCCATGTA CCGGGCTCAG 
CAACTCAGAT CCCATCCAGG CGGGGGCCCG ACTGTGCCCT CCCCTGGCCG CCACATTGTC 
CCAGCCCAGG TTCACGTCAA TGGTGGGGCC CTGGCATCTG AGCGGGAGAC AGACATTCTA 
GATGATGAGC TGCCCATCCA GGACGGGCAG AGTGGGGGCA GCATGGGAAC GCTGTCCTCC 
CTGGATGGGG TCACCAACAC CAGTGAAAGT GGCTACCCAG AGACCCTGTC CCCACTGACC 
AATGGCCTGG ACAAACCCTA TTCCACAGAA CCTGTGCTCA ACGGCGGAGG CTACCCCTAT 
GAGGCTGCCA ACCGGGTGAT TCCTGTCCAC AGCAGTCACT CGGCCCCCAT TCGACCCTCC 
TACTCTGCTC AGGAGGGTTT AGCTGGCTAC CAGCGTGAGG GACCCCACCC AGCCTGGTCA 
CAGCAAGTGA CCAGTGCCCA CTGTGGCTGT GACCCCAGTG GTCTATTCAG ATCCCAGTCC 
TTTCCAGATG TGGAGCCCCA GCTGCCCCAA GCTCCAACCC GTGGGGGTAG TAGCCGAGAG 
GCTGTGCAGA GGGGTCTGAA TTCATGGCAA CAGCAGCAGC CCCACCCACC TCCCCGCCAG 
CAGGAGAGAT CCCCACTGCA GAGTCTTGCC CGCAGCAAGC CTAGCCCCCA GCTGTCAGCA 
GAGACCCCCG TAGCTGCTCT CCCAGAGTTC CCAAGGGCAG CGTCCCAGCA GGAGATCGAG 
CAGTCCATCG AAACCCTCAA TATGTTGATG CTGGACCTGG AGCCAGCCTC CGCAGCTGCC 
CCTCTTCATA AGTCCCAGAG TGTCCCTGGG GCCTGGCCAG GGGCTTCTCC ACTCTCTTCC 
CAGCCCCTCT TGGGGTCTTC CCGCCAGTCC CACCCACTTA CCCAGTCCCG ATCTGGCTAT 
ATCCCCAGTG GGCATTCTCT GGGAACCCCG GAGCTGGTCT CTTCTGGCAG GCCTTATTCA 
CCTTACGATT ATCAGCTGCA TCCAGCTGGA TCCAACCAGA GTTTCCATCC AAAAAGTCCA 
GCCTCTTCCA CCTTCCTTCC AAGCCCTCAT AGCTCTGCGG GGCCTCAGGA GCCCCCAGCC 
TCTCTCCCTG GTCTCATCGC TCAGCCCCAG CTCCCACCAA AGGAAACTAC ATCAGATCCC 
TCCCGAACTC CAGAGGAAGA GCCGCTGAAC CTGGAAGGGC TGGTAGCCCA TCGGGTAGCA 
GTGTACAATG CCAGACTACA GAGCCTGAGG CAGAGCCGCG GCTTCCAACC CTCTCCCCAC 
TGGCGCCGAT CTGCCTCCCT CTCTGGGGTT CAGGCTCGGG AGAGGCAGCC TGCAGAGCCC 
CCAGGCCCAC TCCGGAGGCG GGCAGCCAGT GATGGACAGT ATGAAAACCA GTCTCCGGAG 
GCCACATCCC CCCGTAGTCC TGGGGTCCGC TCCCCCGTCC AGTGCGTCTC CCCTGAGCTG 
GCTCTTACCA TTGCCCTCAA TCCTGGAGGG CGCCCCAAAG AGCCTCATTT GCATAGCTAC 
AAGGAGGCCT TTGAGGAGAT GGAGGGGACC TCCCCAAGCA GCCCGCCACA CAGTGTGGCG 
CGCTCTCCTC CAGGTCTGGC CAAGACACCC CTGTCTGCTC TGGGCCTGAA ACCCCACAAC 
CCAGCAGACA TCTTGCTGCA CCCCACAGGT GTTGCCAGAA GACTGATCCA GCCAGAGGAA 
GACGAGGGGG AGGAGGTGAC TAAGCCTCCA GAAGAGCCCC GGAGCTATGT GGAGTCCGTG 
GCGAGGACAG CGGTGGCAGG GCCTCGAGCT CAGGACGTTG AGCCTAAGAG CTTCAGCGCA 
CCAGCTGCTC ACGCCTATGG GCACGAGACA CCCCTGAGGA ACGGGACCCC AGGGGGCTCC 
TTTGTCTCCC CGAGCCCCCT CTCCACAAGC AGCCCCATCC TCAGTGCTGA CAGCACTTCC 
GTGGGCAGTT TCCCATCAGT GGTGAGCAGT GACCAGGGTC CCCGGACACC CTTCCAGCCT 
ATGCTGGACT CCAGCATCCG CTCAGGCAGC TTGGGGCAGC CGAGCCCTGC AGCACTGAGT 
TATCAGAGCT CCTCGCCTGT CCCTGTTGGT GGCAGCAGCT ACAACAGCCC TGACTACTCC 
CTCCAACCAT TCAGCTCTTC TCCAGAAAGC CAAGGCCAGC CACAGTACAG TGCGGCCAGT 
GTCCACATGG TACCTGGGAG CCCTCAGGCA CGACACAGGA CAGTGGGTAC CAACACTCCA 
CCTAGCCCTG GTTTTGGCCG GCGGGCCGTC AACCCCACCA TGGCTGCCCC TGGTAGTCCC 
AGTTTGAGCC ATCGGCAGGT GATGGGTCCA TCTGGTCCAG GTTTCCATGG TAACGTGGTT 
TCTGGCCACC CAGCCAGTGC AGCTACCACT CCTGGAAGCC CCAGCCTGGG CCGGCACCCA 
GTGGGAAGCC ATCAAGTTCC AGGCCTCCAC AGCAGTGTGG TCACCACTCC GGGAAGCCCA 
AGCCTGGGCC GACACCCTGG TGCCCACCAA GGGAATCTGG CCTCCAGTCT CCACAGCAAT 
GCAGTCATCA GCCCTGGGAG CCCAAGCTTG GGCCGGCACC TGGGTGGGTC TGGATCCGTG 
GTTCCTGGAA GCCCCAGCTT GGACCGGCAT GCAGCTTATG GTGGCTACTC TACCCCTGAG 
GACCGAAGAC CAACACTGTC CCGGCAAAGC AGTGCCTCTG GCTACCAGGC CCCATCCACA 
CCGTCATTCC CTGTCTCTCC TGCCTACTAC CCTGGCCTGA GCAGCCCTGC CACCTCACCC 
TCGCCAGACT CAGCAGCCTT CCGGCAGGGG AGCCCCACAC CAGCCTTACC AGAGAAGCGA 
AGAATGTCAG TGGGAGACCG GGCAGGCAGC CTCCCCAACT ATGCCACCAT CAATGGGAAA 
GTGTCTTCCT CGCCTGTCGC CAATGGCATG GCCAGTGGGA GCAGCACCGT CTCCTTCTCT 
CACACGCTTC CGGACTTTTC TAAGTATTCG ATGCCAGACA ATAGTCCGGA GACTCGGGCT 
AAAGTGAAGT TTGTCCAGGA CACTTCCAAG TATTGGTACA AGCCTGAGAT CTCCAGAGAA 
CAGGCCATCG CACTCCTAAA GGACCAGGAG CCAGGAGCCT TCATCATCCG GGACAGCCAC 
TCCTTCCGAG GGGCTTATGG GCTGGCCATG AAGGTGTCCT CACCCCCTCC AACCATCACT 
CAGCAGGGCA AGAAAGGAGA CATGACCCAT GAGCTGGTGA GGCACTTTCT GATCGAGACA 
GGCCCTAGAG GAGTGAAGCT CAAGGGTTGT CCCAATGAGC CAAACTTTGG TTCCCTTTCT 
GCCCTGGTCT ATCAGCACTC CGTCATCCCA CTGGCCCTGC CCTGTAAACT GGTCATTCCA 
AGCCGAGACC CCACAGATGA ATCAAAAGAT AGCTCAGGCC CTGCCAACTC AACCACTGAC 
CTGCTGAAGC AAGGAGCAGC TTGCAATGTG CTCTTCGTCA ACTCTGTGGA TATGGAGTCA 
CTCACCGGGC CACAGGCCAT TTCCAAAGCC ACATCTGAGA CTCTGGCTGC CGACCCCACA 
CCGGCTGCCA CCATTGTTCA CTTCAAGGTC TCTGCCCAAG GAATCACACT GACTGACAAT 
CAAAGAAAGC TCTTCTTTAG AAGACACTAC CCCCTCAACA CTGTCACCTT CTGTGACCTG 
GATCCACAAG AAAGAAAGTG GATGAAGACA GAGGGAGGCG CCCCTGCTAA GCTCTTTGGC 
TTCGTGGCCC GGAAGCAGGG CAGCACCACA GACAACGCTT GCCACCTCTT CGCAGAGCTT 
GACCCCAACC AGCCTGCGTC TGCCATCGTC AACTTCGTCT CCAAGGTCAT GCTGAGTGCT 
GGCCAGAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495875.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495938.1 Mus musculus tensin 1 (Tns1), transcript variant X18, mRNA.

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CloneID	OMu27363
Clone ID Related Accession (Same CDS sequence)	XM_006495875.2
Accession Version	XM_006495875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006495875.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTGCA GCTTCTCCTG TCATCGGAAA TGCCAGGCCA AGGTGGCTGC CCCTTGCGTT 
CCTCCGTCCA GCCATGAACT GGTGCCCATC ACCACAGAGA CCGTACCCAA GAATGTAGTA 
GATGTGGGGG AAGGAGACTG CCGGGTTGGA AGTTCTCCGA AAAACCTGGA GGAGGGCGGC 
TCCATGAGAG TTTCTCCAAG CATACAGCCA CAACCACAAT CACAGCCTAC CAGTCTCTCT 
AGAAACACGA GTGTGAGCCG AGCCATGGAA GACAGTTGTG AGCTGGACCT GGTGTATGTC 
ACCGAACGCA TCATCGCCGT GTCCTTCCCT AGCACAGCCA ATGAGGAGAA CTTCCGCAGC 
AACCTCCGTG AAGTGGCTCA GATGTTGAAG TCCAAGCATG GGGGCAACTA CCTGCTATTC 
AACCTCTCTG AACAGAGGCC TGACATCACC AAGCTGCATG CTAAGGTCCT GGAATTCGGC 
TGGCCTGACC TGCACACACC GGCCCTGGAG AAGATATGCA GTGTCTGTAA GGCCATGGAC 
ACCTGGCTCA ATGCAGACCC CCACAATGTC GTTGTTCTCC ACAACAAGGG AAACCGAGGC 
AGGATTGGGG TTGTCATTGC AGCGTATCTG CACTACAGCA ACATTTCAGC CAGTGCTGAC 
CAGGCTCTGG ACCGGTTTGC AATGAAGAGA TTCTATGAGG ACAAGATCGT GCCCATTGGC 
CAGCCATCCC AGAGGAGATA TGTGCACTAC TTCAGTGGCC TACTCTCTGG CTCCATCAAA 
ATGAACAACA AGCCTTTGTT TCTGCATCAT GTGATCATGC ACGGCATCCC CAACTTTGAG 
TCTAAAGGAG GGTGCCGGCC CTTTCTCCGC ATCTACCAAG CCATGCAACC TGTGTACACA 
TCTGGCATTT ACAACATCCC TGGAGACAGC CAGGCCAGCA TCTGTATCAC CATTGAACCA 
GGGCTGCTCT TGAAGGGAGA CATCTTGCTG AAGTGTTATC ATAAGAAATT CCGGAGCCCG 
GCTCGAGACG TCATCTTCCG AGTGCAGTTC CACACCTGTG CCATCCATGA CTTGGGGGTT 
GTTTTTGGGA AGGAAGATCT GGATGAGGCC TTCAAAGATG ATCGATTTCC TGACTATGGC 
AAAGTGGAGT TTGTCTTTTC CTATGGACCA GAGAAAATTC AAGGCATGGA ACACCTGGAG 
AATGGGCCCA GCGTATCTGT AGACTATAAC ACTTCTGACC CCCTCATCCG CTGGGACTCC 
TATGACAACT TCAGTGGGCA TCGTGAGGAT GGCATGGAAG GTTTAAAAGT GGTGGGACAC 
ACCCAAGGGC CGCTGGATGG GAGCCTGTAC GCCAAGGTGA AGAAGAAGGA TTCCCTGAAT 
GGCAGTTCGG GGCCTGTCAC TACTGCACGG CCTGCCCTGT CAGCCACTCC CAACCACGTG 
GAACACACCC TGTCTGTGAG CAGCGACTCT GGCAACTCCA CAGCCTCCAC TAAGACAGAC 
AAGACCGATG AGCCAGTCTC TGGGGCTACC ACTGCCCCTG CTGCCCTGAG TCCTCAGGAG 
AAGAAAGAGC TGGACCGCCT ACTAAGTGGC TTTGGTGTGG ATAGAGAAAA GCAAGGCGCC 
ATGTACCGGG CTCAGCAACT CAGATCCCAT CCAGGCGGGG GCCCGACTGT GCCCTCCCCT 
GGCCGCCACA TTGTCCCAGC CCAGGTTCAC GTCAATGGTG GGGCCCTGGC ATCTGAGCGG 
GAGACAGACA TTCTAGATGA TGAGCTGCCC ATCCAGGACG GGCAGAGTGG GGGCAGCATG 
GGAACGCTGT CCTCCCTGGA TGGGGTCACC AACACCAGTG AAAGTGGCTA CCCAGAGACC 
CTGTCCCCAC TGACCAATGG CCTGGACAAA CCCTATTCCA CAGAACCTGT GCTCAACGGC 
GGAGGCTACC CCTATGAGGC TGCCAACCGG GTGATTCCTG TCCACAGCAG TCACTCGGCC 
CCCATTCGAC CCTCCTACTC TGCTCAGGAG GGTTTAGCTG GCTACCAGCG TGAGGGACCC 
CACCCAGCCT GGTCACAGCA AGTGACCAGT GCCCACTGTG GCTGTGACCC CAGTGGTCTA 
TTCAGATCCC AGTCCTTTCC AGATGTGGAG CCCCAGCTGC CCCAAGCTCC AACCCGTGGG 
GGTAGTAGCC GAGAGGCTGT GCAGAGGGGT CTGAATTCAT GGCAACAGCA GCAGCCCCAC 
CCACCTCCCC GCCAGCAGGA GAGATCCCCA CTGCAGAGTC TTGCCCGCAG CAAGCCTAGC 
CCCCAGCTGT CAGCAGAGAC CCCCGTAGCT GCTCTCCCAG AGTTCCCAAG GGCAGCGTCC 
CAGCAGGAGA TCGAGCAGTC CATCGAAACC CTCAATATGT TGATGCTGGA CCTGGAGCCA 
GCCTCCGCAG CTGCCCCTCT TCATAAGTCC CAGAGTGTCC CTGGGGCCTG GCCAGGGGCT 
TCTCCACTCT CTTCCCAGCC CCTCTTGGGG TCTTCCCGCC AGTCCCACCC ACTTACCCAG 
TCCCGATCTG GCTATATCCC CAGTGGGCAT TCTCTGGGAA CCCCGGAGCT GGTCTCTTCT 
GGCAGGCCTT ATTCACCTTA CGATTATCAG CTGCATCCAG CTGGATCCAA CCAGAGTTTC 
CATCCAAAAA GTCCAGCCTC TTCCACCTTC CTTCCAAGCC CTCATAGCTC TGCGGGGCCT 
CAGGAGCCCC CAGCCTCTCT CCCTGGTCTC ATCGCTCAGC CCCAGCTCCC ACCAAAGGAA 
ACTACATCAG ATCCCTCCCG AACTCCAGAG GAAGAGCCGC TGAACCTGGA AGGGCTGGTA 
GCCCATCGGG TAGCAGTGTA CAATGCCAGA CTACAGAGCC TGAGGCAGAG CCGCGGCTTC 
CAACCCTCTC CCCACTGGCG CCGATCTGCC TCCCTCTCTG GGGTTCAGGC TCGGGAGAGG 
CAGCCTGCAG AGCCCCCAGG CCCACTCCGG AGGCGGGCAG CCAGTGATGG ACAGTATGAA 
AACCAGTCTC CGGAGGCCAC ATCCCCCCGT AGTCCTGGGG TCCGCTCCCC CGTCCAGTGC 
GTCTCCCCTG AGCTGGCTCT TACCATTGCC CTCAATCCTG GAGGGCGCCC CAAAGAGCCT 
CATTTGCATA GCTACAAGGA GGCCTTTGAG GAGATGGAGG GGACCTCCCC AAGCAGCCCG 
CCACACAGTG TGGCGCGCTC TCCTCCAGGT CTGGCCAAGA CACCCCTGTC TGCTCTGGGC 
CTGAAACCCC ACAACCCAGC AGACATCTTG CTGCACCCCA CAGGTGTTGC CAGAAGACTG 
ATCCAGCCAG AGGAAGACGA GGGGGAGGAG GTGACTAAGC CTCCAGAAGA GCCCCGGAGC 
TATGTGGAGT CCGTGGCGAG GACAGCGGTG GCAGGGCCTC GAGCTCAGGA CGTTGAGCCT 
AAGAGCTTCA GCGCACCAGC TGCTCACGCC TATGGGCACG AGACACCCCT GAGGAACGGG 
ACCCCAGGGG GCTCCTTTGT CTCCCCGAGC CCCCTCTCCA CAAGCAGCCC CATCCTCAGT 
GCTGACAGCA CTTCCGTGGG CAGTTTCCCA TCAGTGGTGA GCAGTGACCA GGGTCCCCGG 
ACACCCTTCC AGCCTATGCT GGACTCCAGC ATCCGCTCAG GCAGCTTGGG GCAGCCGAGC 
CCTGCAGCAC TGAGTTATCA GAGCTCCTCG CCTGTCCCTG TTGGTGGCAG CAGCTACAAC 
AGCCCTGACT ACTCCCTCCA ACCATTCAGC TCTTCTCCAG AAAGCCAAGG CCAGCCACAG 
TACAGTGCGG CCAGTGTCCA CATGGTACCT GGGAGCCCTC AGGCACGACA CAGGACAGTG 
GGTACCAACA CTCCACCTAG CCCTGGTTTT GGCCGGCGGG CCGTCAACCC CACCATGGCT 
GCCCCTGGTA GTCCCAGTTT GAGCCATCGG CAGGTGATGG GTCCATCTGG TCCAGGTTTC 
CATGGTAACG TGGTTTCTGG CCACCCAGCC AGTGCAGCTA CCACTCCTGG AAGCCCCAGC 
CTGGGCCGGC ACCCAGTGGG AAGCCATCAA GTTCCAGGCC TCCACAGCAG TGTGGTCACC 
ACTCCGGGAA GCCCAAGCCT GGGCCGACAC CCTGGTGCCC ACCAAGGGAA TCTGGCCTCC 
AGTCTCCACA GCAATGCAGT CATCAGCCCT GGGAGCCCAA GCTTGGGCCG GCACCTGGGT 
GGGTCTGGAT CCGTGGTTCC TGGAAGCCCC AGCTTGGACC GGCATGCAGC TTATGGTGGC 
TACTCTACCC CTGAGGACCG AAGACCAACA CTGTCCCGGC AAAGCAGTGC CTCTGGCTAC 
CAGGCCCCAT CCACACCGTC ATTCCCTGTC TCTCCTGCCT ACTACCCTGG CCTGAGCAGC 
CCTGCCACCT CACCCTCGCC AGACTCAGCA GCCTTCCGGC AGGGGAGCCC CACACCAGCC 
TTACCAGAGA AGCGAAGAAT GTCAGTGGGA GACCGGGCAG GCAGCCTCCC CAACTATGCC 
ACCATCAATG GGAAAGTGTC TTCCTCGCCT GTCGCCAATG GCATGGCCAG TGGGAGCAGC 
ACCGTCTCCT TCTCTCACAC GCTTCCGGAC TTTTCTAAGT ATTCGATGCC AGACAATAGT 
CCGGAGACTC GGGCTAAAGT GAAGTTTGTC CAGGACACTT CCAAGTATTG GTACAAGCCT 
GAGATCTCCA GAGAACAGGC CATCGCACTC CTAAAGGACC AGGAGCCAGG AGCCTTCATC 
ATCCGGGACA GCCACTCCTT CCGAGGGGCT TATGGGCTGG CCATGAAGGT GTCCTCACCC 
CCTCCAACCA TCACTCAGCA GGGCAAGAAA GGAGACATGA CCCATGAGCT GGTGAGGCAC 
TTTCTGATCG AGACAGGCCC TAGAGGAGTG AAGCTCAAGG GTTGTCCCAA TGAGCCAAAC 
TTTGGTTCCC TTTCTGCCCT GGTCTATCAG CACTCCGTCA TCCCACTGGC CCTGCCCTGT 
AAACTGGTCA TTCCAAGCCG AGACCCCACA GATGAATCAA AAGATAGCTC AGGCCCTGCC 
AACTCAACCA CTGACCTGCT GAAGCAAGGA GCAGCTTGCA ATGTGCTCTT CGTCAACTCT 
GTGGATATGG AGTCACTCAC CGGGCCACAG GCCATTTCCA AAGCCACATC TGAGACTCTG 
GCTGCCGACC CCACACCGGC TGCCACCATT GTTCACTTCA AGGTCTCTGC CCAAGGAATC 
ACACTGACTG ACAATCAAAG AAAGCTCTTC TTTAGAAGAC ACTACCCCCT CAACACTGTC 
ACCTTCTGTG ACCTGGATCC ACAAGAAAGA AAGTGGATGA AGACAGAGGG AGGCGCCCCT 
GCTAAGCTCT TTGGCTTCGT GGCCCGGAAG CAGGGCAGCA CCACAGACAA CGCTTGCCAC 
CTCTTCGCAG AGCTTGACCC CAACCAGCCT GCGTCTGCCA TCGTCAACTT CGTCTCCAAG 
GTCATGCTGA GTGCTGGCCA GAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495938.1
CDS	16..5505
Translation	MVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495875.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495875.2

ORF Insert Sequence:

ATGGTCTGCA GCTTCTCCTG TCATCGGAAA TGCCAGGCCA AGGTGGCTGC CCCTTGCGTT 
CCTCCGTCCA GCCATGAACT GGTGCCCATC ACCACAGAGA CCGTACCCAA GAATGTAGTA 
GATGTGGGGG AAGGAGACTG CCGGGTTGGA AGTTCTCCGA AAAACCTGGA GGAGGGCGGC 
TCCATGAGAG TTTCTCCAAG CATACAGCCA CAACCACAAT CACAGCCTAC CAGTCTCTCT 
AGAAACACGA GTGTGAGCCG AGCCATGGAA GACAGTTGTG AGCTGGACCT GGTGTATGTC 
ACCGAACGCA TCATCGCCGT GTCCTTCCCT AGCACAGCCA ATGAGGAGAA CTTCCGCAGC 
AACCTCCGTG AAGTGGCTCA GATGTTGAAG TCCAAGCATG GGGGCAACTA CCTGCTATTC 
AACCTCTCTG AACAGAGGCC TGACATCACC AAGCTGCATG CTAAGGTCCT GGAATTCGGC 
TGGCCTGACC TGCACACACC GGCCCTGGAG AAGATATGCA GTGTCTGTAA GGCCATGGAC 
ACCTGGCTCA ATGCAGACCC CCACAATGTC GTTGTTCTCC ACAACAAGGG AAACCGAGGC 
AGGATTGGGG TTGTCATTGC AGCGTATCTG CACTACAGCA ACATTTCAGC CAGTGCTGAC 
CAGGCTCTGG ACCGGTTTGC AATGAAGAGA TTCTATGAGG ACAAGATCGT GCCCATTGGC 
CAGCCATCCC AGAGGAGATA TGTGCACTAC TTCAGTGGCC TACTCTCTGG CTCCATCAAA 
ATGAACAACA AGCCTTTGTT TCTGCATCAT GTGATCATGC ACGGCATCCC CAACTTTGAG 
TCTAAAGGAG GGTGCCGGCC CTTTCTCCGC ATCTACCAAG CCATGCAACC TGTGTACACA 
TCTGGCATTT ACAACATCCC TGGAGACAGC CAGGCCAGCA TCTGTATCAC CATTGAACCA 
GGGCTGCTCT TGAAGGGAGA CATCTTGCTG AAGTGTTATC ATAAGAAATT CCGGAGCCCG 
GCTCGAGACG TCATCTTCCG AGTGCAGTTC CACACCTGTG CCATCCATGA CTTGGGGGTT 
GTTTTTGGGA AGGAAGATCT GGATGAGGCC TTCAAAGATG ATCGATTTCC TGACTATGGC 
AAAGTGGAGT TTGTCTTTTC CTATGGACCA GAGAAAATTC AAGGCATGGA ACACCTGGAG 
AATGGGCCCA GCGTATCTGT AGACTATAAC ACTTCTGACC CCCTCATCCG CTGGGACTCC 
TATGACAACT TCAGTGGGCA TCGTGAGGAT GGCATGGAAG GTTTAAAAGT GGTGGGACAC 
ACCCAAGGGC CGCTGGATGG GAGCCTGTAC GCCAAGGTGA AGAAGAAGGA TTCCCTGAAT 
GGCAGTTCGG GGCCTGTCAC TACTGCACGG CCTGCCCTGT CAGCCACTCC CAACCACGTG 
GAACACACCC TGTCTGTGAG CAGCGACTCT GGCAACTCCA CAGCCTCCAC TAAGACAGAC 
AAGACCGATG AGCCAGTCTC TGGGGCTACC ACTGCCCCTG CTGCCCTGAG TCCTCAGGAG 
AAGAAAGAGC TGGACCGCCT ACTAAGTGGC TTTGGTGTGG ATAGAGAAAA GCAAGGCGCC 
ATGTACCGGG CTCAGCAACT CAGATCCCAT CCAGGCGGGG GCCCGACTGT GCCCTCCCCT 
GGCCGCCACA TTGTCCCAGC CCAGGTTCAC GTCAATGGTG GGGCCCTGGC ATCTGAGCGG 
GAGACAGACA TTCTAGATGA TGAGCTGCCC ATCCAGGACG GGCAGAGTGG GGGCAGCATG 
GGAACGCTGT CCTCCCTGGA TGGGGTCACC AACACCAGTG AAAGTGGCTA CCCAGAGACC 
CTGTCCCCAC TGACCAATGG CCTGGACAAA CCCTATTCCA CAGAACCTGT GCTCAACGGC 
GGAGGCTACC CCTATGAGGC TGCCAACCGG GTGATTCCTG TCCACAGCAG TCACTCGGCC 
CCCATTCGAC CCTCCTACTC TGCTCAGGAG GGTTTAGCTG GCTACCAGCG TGAGGGACCC 
CACCCAGCCT GGTCACAGCA AGTGACCAGT GCCCACTGTG GCTGTGACCC CAGTGGTCTA 
TTCAGATCCC AGTCCTTTCC AGATGTGGAG CCCCAGCTGC CCCAAGCTCC AACCCGTGGG 
GGTAGTAGCC GAGAGGCTGT GCAGAGGGGT CTGAATTCAT GGCAACAGCA GCAGCCCCAC 
CCACCTCCCC GCCAGCAGGA GAGATCCCCA CTGCAGAGTC TTGCCCGCAG CAAGCCTAGC 
CCCCAGCTGT CAGCAGAGAC CCCCGTAGCT GCTCTCCCAG AGTTCCCAAG GGCAGCGTCC 
CAGCAGGAGA TCGAGCAGTC CATCGAAACC CTCAATATGT TGATGCTGGA CCTGGAGCCA 
GCCTCCGCAG CTGCCCCTCT TCATAAGTCC CAGAGTGTCC CTGGGGCCTG GCCAGGGGCT 
TCTCCACTCT CTTCCCAGCC CCTCTTGGGG TCTTCCCGCC AGTCCCACCC ACTTACCCAG 
TCCCGATCTG GCTATATCCC CAGTGGGCAT TCTCTGGGAA CCCCGGAGCT GGTCTCTTCT 
GGCAGGCCTT ATTCACCTTA CGATTATCAG CTGCATCCAG CTGGATCCAA CCAGAGTTTC 
CATCCAAAAA GTCCAGCCTC TTCCACCTTC CTTCCAAGCC CTCATAGCTC TGCGGGGCCT 
CAGGAGCCCC CAGCCTCTCT CCCTGGTCTC ATCGCTCAGC CCCAGCTCCC ACCAAAGGAA 
ACTACATCAG ATCCCTCCCG AACTCCAGAG GAAGAGCCGC TGAACCTGGA AGGGCTGGTA 
GCCCATCGGG TAGCAGTGTA CAATGCCAGA CTACAGAGCC TGAGGCAGAG CCGCGGCTTC 
CAACCCTCTC CCCACTGGCG CCGATCTGCC TCCCTCTCTG GGGTTCAGGC TCGGGAGAGG 
CAGCCTGCAG AGCCCCCAGG CCCACTCCGG AGGCGGGCAG CCAGTGATGG ACAGTATGAA 
AACCAGTCTC CGGAGGCCAC ATCCCCCCGT AGTCCTGGGG TCCGCTCCCC CGTCCAGTGC 
GTCTCCCCTG AGCTGGCTCT TACCATTGCC CTCAATCCTG GAGGGCGCCC CAAAGAGCCT 
CATTTGCATA GCTACAAGGA GGCCTTTGAG GAGATGGAGG GGACCTCCCC AAGCAGCCCG 
CCACACAGTG TGGCGCGCTC TCCTCCAGGT CTGGCCAAGA CACCCCTGTC TGCTCTGGGC 
CTGAAACCCC ACAACCCAGC AGACATCTTG CTGCACCCCA CAGGTGTTGC CAGAAGACTG 
ATCCAGCCAG AGGAAGACGA GGGGGAGGAG GTGACTAAGC CTCCAGAAGA GCCCCGGAGC 
TATGTGGAGT CCGTGGCGAG GACAGCGGTG GCAGGGCCTC GAGCTCAGGA CGTTGAGCCT 
AAGAGCTTCA GCGCACCAGC TGCTCACGCC TATGGGCACG AGACACCCCT GAGGAACGGG 
ACCCCAGGGG GCTCCTTTGT CTCCCCGAGC CCCCTCTCCA CAAGCAGCCC CATCCTCAGT 
GCTGACAGCA CTTCCGTGGG CAGTTTCCCA TCAGTGGTGA GCAGTGACCA GGGTCCCCGG 
ACACCCTTCC AGCCTATGCT GGACTCCAGC ATCCGCTCAG GCAGCTTGGG GCAGCCGAGC 
CCTGCAGCAC TGAGTTATCA GAGCTCCTCG CCTGTCCCTG TTGGTGGCAG CAGCTACAAC 
AGCCCTGACT ACTCCCTCCA ACCATTCAGC TCTTCTCCAG AAAGCCAAGG CCAGCCACAG 
TACAGTGCGG CCAGTGTCCA CATGGTACCT GGGAGCCCTC AGGCACGACA CAGGACAGTG 
GGTACCAACA CTCCACCTAG CCCTGGTTTT GGCCGGCGGG CCGTCAACCC CACCATGGCT 
GCCCCTGGTA GTCCCAGTTT GAGCCATCGG CAGGTGATGG GTCCATCTGG TCCAGGTTTC 
CATGGTAACG TGGTTTCTGG CCACCCAGCC AGTGCAGCTA CCACTCCTGG AAGCCCCAGC 
CTGGGCCGGC ACCCAGTGGG AAGCCATCAA GTTCCAGGCC TCCACAGCAG TGTGGTCACC 
ACTCCGGGAA GCCCAAGCCT GGGCCGACAC CCTGGTGCCC ACCAAGGGAA TCTGGCCTCC 
AGTCTCCACA GCAATGCAGT CATCAGCCCT GGGAGCCCAA GCTTGGGCCG GCACCTGGGT 
GGGTCTGGAT CCGTGGTTCC TGGAAGCCCC AGCTTGGACC GGCATGCAGC TTATGGTGGC 
TACTCTACCC CTGAGGACCG AAGACCAACA CTGTCCCGGC AAAGCAGTGC CTCTGGCTAC 
CAGGCCCCAT CCACACCGTC ATTCCCTGTC TCTCCTGCCT ACTACCCTGG CCTGAGCAGC 
CCTGCCACCT CACCCTCGCC AGACTCAGCA GCCTTCCGGC AGGGGAGCCC CACACCAGCC 
TTACCAGAGA AGCGAAGAAT GTCAGTGGGA GACCGGGCAG GCAGCCTCCC CAACTATGCC 
ACCATCAATG GGAAAGTGTC TTCCTCGCCT GTCGCCAATG GCATGGCCAG TGGGAGCAGC 
ACCGTCTCCT TCTCTCACAC GCTTCCGGAC TTTTCTAAGT ATTCGATGCC AGACAATAGT 
CCGGAGACTC GGGCTAAAGT GAAGTTTGTC CAGGACACTT CCAAGTATTG GTACAAGCCT 
GAGATCTCCA GAGAACAGGC CATCGCACTC CTAAAGGACC AGGAGCCAGG AGCCTTCATC 
ATCCGGGACA GCCACTCCTT CCGAGGGGCT TATGGGCTGG CCATGAAGGT GTCCTCACCC 
CCTCCAACCA TCACTCAGCA GGGCAAGAAA GGAGACATGA CCCATGAGCT GGTGAGGCAC 
TTTCTGATCG AGACAGGCCC TAGAGGAGTG AAGCTCAAGG GTTGTCCCAA TGAGCCAAAC 
TTTGGTTCCC TTTCTGCCCT GGTCTATCAG CACTCCGTCA TCCCACTGGC CCTGCCCTGT 
AAACTGGTCA TTCCAAGCCG AGACCCCACA GATGAATCAA AAGATAGCTC AGGCCCTGCC 
AACTCAACCA CTGACCTGCT GAAGCAAGGA GCAGCTTGCA ATGTGCTCTT CGTCAACTCT 
GTGGATATGG AGTCACTCAC CGGGCCACAG GCCATTTCCA AAGCCACATC TGAGACTCTG 
GCTGCCGACC CCACACCGGC TGCCACCATT GTTCACTTCA AGGTCTCTGC CCAAGGAATC 
ACACTGACTG ACAATCAAAG AAAGCTCTTC TTTAGAAGAC ACTACCCCCT CAACACTGTC 
ACCTTCTGTG ACCTGGATCC ACAAGAAAGA AAGTGGATGA AGACAGAGGG AGGCGCCCCT 
GCTAAGCTCT TTGGCTTCGT GGCCCGGAAG CAGGGCAGCA CCACAGACAA CGCTTGCCAC 
CTCTTCGCAG AGCTTGACCC CAACCAGCCT GCGTCTGCCA TCGTCAACTT CGTCTCCAAG 
GTCATGCTGA GTGCTGGCCA GAAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495880.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495943.1 Mus musculus tensin 1 (Tns1), transcript variant X23, mRNA.

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CloneID	OMu27365
Clone ID Related Accession (Same CDS sequence)	XM_006495880.2 , XM_011238476.2 , XM_006495880.3 , XM_011238477.1 , XM_006495881.3 , XM_006495877.3 , XM_006495881.2 , XM_011238476.1 , XM_006495877.4 , XM_006495879.1 , XM_006495878.3 , XM_006495882.3
Accession Version	XM_006495880.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006495880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495943.1
CDS	243..5549
Translation	MRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495880.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495880.2

ORF Insert Sequence:

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_011238477.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236779.1 Mus musculus tensin 1 (Tns1), transcript variant X27, mRNA.

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CloneID	OMu27365
Clone ID Related Accession (Same CDS sequence)	XM_006495880.2 , XM_011238476.2 , XM_006495880.3 , XM_011238477.1 , XM_006495881.3 , XM_006495877.3 , XM_006495881.2 , XM_011238476.1 , XM_006495877.4 , XM_006495879.1 , XM_006495878.3 , XM_006495882.3
Accession Version	XM_011238477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236779.1
CDS	167..5473
Translation	MRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_011238477.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238477.1

ORF Insert Sequence:

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495877.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495940.1 Mus musculus tensin 1 (Tns1), transcript variant X19, mRNA.

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CloneID	OMu27365
Clone ID Related Accession (Same CDS sequence)	XM_006495880.2 , XM_011238476.2 , XM_006495880.3 , XM_011238477.1 , XM_006495881.3 , XM_006495877.3 , XM_006495881.2 , XM_011238476.1 , XM_006495877.4 , XM_006495879.1 , XM_006495878.3 , XM_006495882.3
Accession Version	XM_006495877.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495877.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495940.1
CDS	360..5666
Translation	MRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495877.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495877.3

ORF Insert Sequence:

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495881.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495944.1 Mus musculus tensin 1 (Tns1), transcript variant X26, mRNA.

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CloneID	OMu27365
Clone ID Related Accession (Same CDS sequence)	XM_006495880.2 , XM_011238476.2 , XM_006495880.3 , XM_011238477.1 , XM_006495881.3 , XM_006495877.3 , XM_006495881.2 , XM_011238476.1 , XM_006495877.4 , XM_006495879.1 , XM_006495878.3 , XM_006495882.3
Accession Version	XM_006495881.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006495881.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495944.1
CDS	413..5719
Translation	MRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495881.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495881.2

ORF Insert Sequence:

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_011238476.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236778.1 Mus musculus tensin 1 (Tns1), transcript variant X21, mRNA.

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CloneID	OMu27365
Clone ID Related Accession (Same CDS sequence)	XM_006495880.2 , XM_011238476.2 , XM_006495880.3 , XM_011238477.1 , XM_006495881.3 , XM_006495877.3 , XM_006495881.2 , XM_011238476.1 , XM_006495877.4 , XM_006495879.1 , XM_006495878.3 , XM_006495882.3
Accession Version	XM_011238476.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236778.1
CDS	344..5650
Translation	MRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_011238476.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238476.1

ORF Insert Sequence:

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495879.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495942.1 Mus musculus tensin 1 (Tns1), transcript variant X26, mRNA.

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CloneID	OMu27365
Clone ID Related Accession (Same CDS sequence)	XM_006495880.2 , XM_011238476.2 , XM_006495880.3 , XM_011238477.1 , XM_006495881.3 , XM_006495877.3 , XM_006495881.2 , XM_011238476.1 , XM_006495877.4 , XM_006495879.1 , XM_006495878.3 , XM_006495882.3
Accession Version	XM_006495879.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495942.1
CDS	166..5472
Translation	MRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495879.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495879.1

ORF Insert Sequence:

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495878.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495941.1 Mus musculus tensin 1 (Tns1), transcript variant X23, mRNA.

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CloneID	OMu27365
Clone ID Related Accession (Same CDS sequence)	XM_006495880.2 , XM_011238476.2 , XM_006495880.3 , XM_011238477.1 , XM_006495881.3 , XM_006495877.3 , XM_006495881.2 , XM_011238476.1 , XM_006495877.4 , XM_006495879.1 , XM_006495878.3 , XM_006495882.3
Accession Version	XM_006495878.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495878.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495941.1
CDS	315..5621
Translation	MRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495878.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495878.3

ORF Insert Sequence:

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495882.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495945.1 Mus musculus tensin 1 (Tns1), transcript variant X28, mRNA.

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CloneID	OMu27365
Clone ID Related Accession (Same CDS sequence)	XM_006495880.2 , XM_011238476.2 , XM_006495880.3 , XM_011238477.1 , XM_006495881.3 , XM_006495877.3 , XM_006495881.2 , XM_011238476.1 , XM_006495877.4 , XM_006495879.1 , XM_006495878.3 , XM_006495882.3
Accession Version	XM_006495882.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495882.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495945.1
CDS	270..5576
Translation	MRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495882.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495882.3

ORF Insert Sequence:

ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495867.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495930.2 Mus musculus tensin 1 (Tns1), transcript variant X1, mRNA.

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CloneID	OMu68935
Clone ID Related Accession (Same CDS sequence)	XM_006495867.2
Accession Version	XM_006495867.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006495867.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGTGTAC 
AATGCCAGAC TACAGAGCCT GAGGCAGAGC CGCGGCTTCC AACCCTCTCC CCACTGGCGC 
CGATCTGCCT CCCTCTCTGG GGTTCAGGCT CGGGAGAGGC AGCCTGCAGA GCCCCCAGGC 
CCACTCCGGA GGCGGGCAGC CAGTGATGGA CAGTATGAAA ACCAGTCTCC GGAGGCCACA 
TCCCCCCGTA GTCCTGGGGT CCGCTCCCCC GTCCAGTGCG TCTCCCCTGA GCTGGCTCTT 
ACCATTGCCC TCAATCCTGG AGGGCGCCCC AAAGAGCCTC ATTTGCATAG CTACAAGGAG 
GCCTTTGAGG AGATGGAGGG GACCTCCCCA AGCAGCCCGC CACACAGTGT GGCGCGCTCT 
CCTCCAGGTC TGGCCAAGAC ACCCCTGTCT GCTCTGGGCC TGAAACCCCA CAACCCAGCA 
GACATCTTGC TGCACCCCAC AGGTGTTGCC AGAAGACTGA TCCAGCCAGA GGAAGACGAG 
GGGGAGGAGG TGACTAAGCC TCCAGAAGAG CCCCGGAGCT ATGTGGAGTC CGTGGCGAGG 
ACAGCGGTGG CAGGGCCTCG AGCTCAGGAC GTTGAGCCTA AGAGCTTCAG CGCACCAGCT 
GCTCACGCCT ATGGGCACGA GACACCCCTG AGGAACGGGA CCCCAGGGGG CTCCTTTGTC 
TCCCCGAGCC CCCTCTCCAC AAGCAGCCCC ATCCTCAGTG CTGACAGCAC TTCCGTGGGC 
AGTTTCCCAT CAGTGGTGAG CAGTGACCAG GGTCCCCGGA CACCCTTCCA GCCTATGCTG 
GACTCCAGCA TCCGCTCAGG CAGCTTGGGG CAGCCGAGCC CTGCAGCACT GAGTTATCAG 
AGCTCCTCGC CTGTCCCTGT TGGTGGCAGC AGCTACAACA GCCCTGACTA CTCCCTCCAA 
CCATTCAGCT CTTCTCCAGA AAGCCAAGGC CAGCCACAGT ACAGTGCGGC CAGTGTCCAC 
ATGGTACCTG GGAGCCCTCA GGCACGACAC AGGACAGTGG GTACCAACAC TCCACCTAGC 
CCTGGTTTTG GCCGGCGGGC CGTCAACCCC ACCATGGCTG CCCCTGGTAG TCCCAGTTTG 
AGCCATCGGC AGGTGATGGG TCCATCTGGT CCAGGTTTCC ATGGTAACGT GGTTTCTGGC 
CACCCAGCCA GTGCAGCTAC CACTCCTGGA AGCCCCAGCC TGGGCCGGCA CCCAGTGGGA 
AGCCATCAAG TTCCAGGCCT CCACAGCAGT GTGGTCACCA CTCCGGGAAG CCCAAGCCTG 
GGCCGACACC CTGGTGCCCA CCAAGGGAAT CTGGCCTCCA GTCTCCACAG CAATGCAGTC 
ATCAGCCCTG GGAGCCCAAG CTTGGGCCGG CACCTGGGTG GGTCTGGATC CGTGGTTCCT 
GGAAGCCCCA GCTTGGACCG GCATGCAGCT TATGGTGGCT ACTCTACCCC TGAGGACCGA 
AGACCAACAC TGTCCCGGCA AAGCAGTGCC TCTGGCTACC AGGCCCCATC CACACCGTCA 
TTCCCTGTCT CTCCTGCCTA CTACCCTGGC CTGAGCAGCC CTGCCACCTC ACCCTCGCCA 
GACTCAGCAG CCTTCCGGCA GGGGAGCCCC ACACCAGCCT TACCAGAGAA GCGAAGAATG 
TCAGTGGGAG ACCGGGCAGG CAGCCTCCCC AACTATGCCA CCATCAATGG GAAAGTGTCT 
TCCTCGCCTG TCGCCAATGG CATGGCCAGT GGGAGCAGCA CCGTCTCCTT CTCTCACACG 
CTTCCGGACT TTTCTAAGTA TTCGATGCCA GACAATAGTC CGGAGACTCG GGCTAAAGTG 
AAGTTTGTCC AGGACACTTC CAAGTATTGG TACAAGCCTG AGATCTCCAG AGAACAGGCC 
ATCGCACTCC TAAAGGACCA GGAGCCAGGA GCCTTCATCA TCCGGGACAG CCACTCCTTC 
CGAGGGGCTT ATGGGCTGGC CATGAAGGTG TCCTCACCCC CTCCAACCAT CACTCAGCAG 
GGCAAGAAAG GAGACATGAC CCATGAGCTG GTGAGGCACT TTCTGATCGA GACAGGCCCT 
AGAGGAGTGA AGCTCAAGGG TTGTCCCAAT GAGCCAAACT TTGGTTCCCT TTCTGCCCTG 
GTCTATCAGC ACTCCGTCAT CCCACTGGCC CTGCCCTGTA AACTGGTCAT TCCAAGCCGA 
GACCCCACAG ATGAATCAAA AGATAGCTCA GGCCCTGCCA ACTCAACCAC TGACCTGCTG 
AAGCAAGGAG CAGCTTGCAA TGTGCTCTTC GTCAACTCTG TGGATATGGA GTCACTCACC 
GGGCCACAGG CCATTTCCAA AGCCACATCT GAGACTCTGG CTGCCGACCC CACACCGGCT 
GCCACCATTG TTCACTTCAA GGTCTCTGCC CAAGGAATCA CACTGACTGA CAATCAAAGA 
AAGCTCTTCT TTAGAAGACA CTACCCCCTC AACACTGTCA CCTTCTGTGA CCTGGATCCA 
CAAGAAAGAA AGTGGATGAA GACAGAGGGA GGCGCCCCTG CTAAGCTCTT TGGCTTCGTG 
GCCCGGAAGC AGGGCAGCAC CACAGACAAC GCTTGCCACC TCTTCGCAGA GCTTGACCCC 
AACCAGCCTG CGTCTGCCAT CGTCAACTTC GTCTCCAAGG TCATGCTGAG TGCTGGCCAG 
AAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495930.2
CDS	16..5784
Translation	MLGDWGKGRGQPTGSPCPPWEHILFWNLGVSLPPCCSRESGVPWTQGALRRKLLVLPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495867.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495867.2

ORF Insert Sequence:

ATGCTTGGTG ACTGGGGCAA AGGCAGGGGC CAACCCACAG GTTCCCCATG TCCTCCTTGG 
GAACACATCC TCTTCTGGAA CCTTGGGGTC TCCCTACCAC CCTGCTGCAG TAGGGAGTCT 
GGAGTGCCAT GGACCCAAGG AGCTCTCAGG AGGAAACTGC TGGTGCTGCC AGAAGACCTG 
GAGGCCCCCA AGACCCACCA CTTCAAGGTG AAGGCCTTCA AGAAGGTGAA GCCCTGTGGC 
ATCTGCCGGC AGGCCATCAC TCGGGAAGGC TGTGTTTGCA AAGTCTGCAG CTTCTCCTGT 
CATCGGAAAT GCCAGGCCAA GGTGGCTGCC CCTTGCGTTC CTCCGTCCAG CCATGAACTG 
GTGCCCATCA CCACAGAGAC CGTACCCAAG AATGTAGTAG ATGTGGGGGA AGGAGACTGC 
CGGGTTGGAA GTTCTCCGAA AAACCTGGAG GAGGGCGGCT CCATGAGAGT TTCTCCAAGC 
ATACAGCCAC AACCACAATC ACAGCCTACC AGTCTCTCTA GAAACACGAG TGTGAGCCGA 
GCCATGGAAG ACAGTTGTGA GCTGGACCTG GTGTATGTCA CCGAACGCAT CATCGCCGTG 
TCCTTCCCTA GCACAGCCAA TGAGGAGAAC TTCCGCAGCA ACCTCCGTGA AGTGGCTCAG 
ATGTTGAAGT CCAAGCATGG GGGCAACTAC CTGCTATTCA ACCTCTCTGA ACAGAGGCCT 
GACATCACCA AGCTGCATGC TAAGGTCCTG GAATTCGGCT GGCCTGACCT GCACACACCG 
GCCCTGGAGA AGATATGCAG TGTCTGTAAG GCCATGGACA CCTGGCTCAA TGCAGACCCC 
CACAATGTCG TTGTTCTCCA CAACAAGGGA AACCGAGGCA GGATTGGGGT TGTCATTGCA 
GCGTATCTGC ACTACAGCAA CATTTCAGCC AGTGCTGACC AGGCTCTGGA CCGGTTTGCA 
ATGAAGAGAT TCTATGAGGA CAAGATCGTG CCCATTGGCC AGCCATCCCA GAGGAGATAT 
GTGCACTACT TCAGTGGCCT ACTCTCTGGC TCCATCAAAA TGAACAACAA GCCTTTGTTT 
CTGCATCATG TGATCATGCA CGGCATCCCC AACTTTGAGT CTAAAGGAGG GTGCCGGCCC 
TTTCTCCGCA TCTACCAAGC CATGCAACCT GTGTACACAT CTGGCATTTA CAACATCCCT 
GGAGACAGCC AGGCCAGCAT CTGTATCACC ATTGAACCAG GGCTGCTCTT GAAGGGAGAC 
ATCTTGCTGA AGTGTTATCA TAAGAAATTC CGGAGCCCGG CTCGAGACGT CATCTTCCGA 
GTGCAGTTCC ACACCTGTGC CATCCATGAC TTGGGGGTTG TTTTTGGGAA GGAAGATCTG 
GATGAGGCCT TCAAAGATGA TCGATTTCCT GACTATGGCA AAGTGGAGTT TGTCTTTTCC 
TATGGACCAG AGAAAATTCA AGGCATGGAA CACCTGGAGA ATGGGCCCAG CGTATCTGTA 
GACTATAACA CTTCTGACCC CCTCATCCGC TGGGACTCCT ATGACAACTT CAGTGGGCAT 
CGTGAGGATG GCATGGAAGG TTTAAAAGTG GTGGGACACA CCCAAGGGCC GCTGGATGGG 
AGCCTGTACG CCAAGGTGAA GAAGAAGGAT TCCCTGAATG GCAGTTCGGG GCCTGTCACT 
ACTGCACGGC CTGCCCTGTC AGCCACTCCC AACCACGTGG AACACACCCT GTCTGTGAGC 
AGCGACTCTG GCAACTCCAC AGCCTCCACT AAGACAGACA AGACCGATGA GCCAGTCTCT 
GGGGCTACCA CTGCCCCTGC TGCCCTGAGT CCTCAGGAGA AGAAAGAGCT GGACCGCCTA 
CTAAGTGGCT TTGGTGTGGA TAGAGAAAAG CAAGGCGCCA TGTACCGGGC TCAGCAACTC 
AGATCCCATC CAGGCGGGGG CCCGACTGTG CCCTCCCCTG GCCGCCACAT TGTCCCAGCC 
CAGGTTCACG TCAATGGTGG GGCCCTGGCA TCTGAGCGGG AGACAGACAT TCTAGATGAT 
GAGCTGCCCA TCCAGGACGG GCAGAGTGGG GGCAGCATGG GAACGCTGTC CTCCCTGGAT 
GGGGTCACCA ACACCAGTGA AAGTGGCTAC CCAGAGACCC TGTCCCCACT GACCAATGGC 
CTGGACAAAC CCTATTCCAC AGAACCTGTG CTCAACGGCG GAGGCTACCC CTATGAGGCT 
GCCAACCGGG TGATTCCTGT CCACAGCAGT CACTCGGCCC CCATTCGACC CTCCTACTCT 
GCTCAGGAGG GTTTAGCTGG CTACCAGCGT GAGGGACCCC ACCCAGCCTG GTCACAGCAA 
GTGACCAGTG CCCACTGTGG CTGTGACCCC AGTGGTCTAT TCAGATCCCA GTCCTTTCCA 
GATGTGGAGC CCCAGCTGCC CCAAGCTCCA ACCCGTGGGG GTAGTAGCCG AGAGGCTGTG 
CAGAGGGGTC TGAATTCATG GCAACAGCAG CAGCCCCACC CACCTCCCCG CCAGCAGGAG 
AGATCCCCAC TGCAGAGTCT TGCCCGCAGC AAGCCTAGCC CCCAGCTGTC AGCAGAGACC 
CCCGTAGCTG CTCTCCCAGA GTTCCCAAGG GCAGCGTCCC AGCAGGAGAT CGAGCAGTCC 
ATCGAAACCC TCAATATGTT GATGCTGGAC CTGGAGCCAG CCTCCGCAGC TGCCCCTCTT 
CATAAGTCCC AGAGTGTCCC TGGGGCCTGG CCAGGGGCTT CTCCACTCTC TTCCCAGCCC 
CTCTTGGGGT CTTCCCGCCA GTCCCACCCA CTTACCCAGT CCCGATCTGG CTATATCCCC 
AGTGGGCATT CTCTGGGAAC CCCGGAGCTG GTCTCTTCTG GCAGGCCTTA TTCACCTTAC 
GATTATCAGC TGCATCCAGC TGGATCCAAC CAGAGTTTCC ATCCAAAAAG TCCAGCCTCT 
TCCACCTTCC TTCCAAGCCC TCATAGCTCT GCGGGGCCTC AGGAGCCCCC AGCCTCTCTC 
CCTGGTCTCA TCGCTCAGCC CCAGCTCCCA CCAAAGGAAA CTACATCAGA TCCCTCCCGA 
ACTCCAGAGG AAGAGCCGCT GAACCTGGAA GGGCTGGTAG CCCATCGGGT AGCAGTGTAC 
AATGCCAGAC TACAGAGCCT GAGGCAGAGC CGCGGCTTCC AACCCTCTCC CCACTGGCGC 
CGATCTGCCT CCCTCTCTGG GGTTCAGGCT CGGGAGAGGC AGCCTGCAGA GCCCCCAGGC 
CCACTCCGGA GGCGGGCAGC CAGTGATGGA CAGTATGAAA ACCAGTCTCC GGAGGCCACA 
TCCCCCCGTA GTCCTGGGGT CCGCTCCCCC GTCCAGTGCG TCTCCCCTGA GCTGGCTCTT 
ACCATTGCCC TCAATCCTGG AGGGCGCCCC AAAGAGCCTC ATTTGCATAG CTACAAGGAG 
GCCTTTGAGG AGATGGAGGG GACCTCCCCA AGCAGCCCGC CACACAGTGT GGCGCGCTCT 
CCTCCAGGTC TGGCCAAGAC ACCCCTGTCT GCTCTGGGCC TGAAACCCCA CAACCCAGCA 
GACATCTTGC TGCACCCCAC AGGTGTTGCC AGAAGACTGA TCCAGCCAGA GGAAGACGAG 
GGGGAGGAGG TGACTAAGCC TCCAGAAGAG CCCCGGAGCT ATGTGGAGTC CGTGGCGAGG 
ACAGCGGTGG CAGGGCCTCG AGCTCAGGAC GTTGAGCCTA AGAGCTTCAG CGCACCAGCT 
GCTCACGCCT ATGGGCACGA GACACCCCTG AGGAACGGGA CCCCAGGGGG CTCCTTTGTC 
TCCCCGAGCC CCCTCTCCAC AAGCAGCCCC ATCCTCAGTG CTGACAGCAC TTCCGTGGGC 
AGTTTCCCAT CAGTGGTGAG CAGTGACCAG GGTCCCCGGA CACCCTTCCA GCCTATGCTG 
GACTCCAGCA TCCGCTCAGG CAGCTTGGGG CAGCCGAGCC CTGCAGCACT GAGTTATCAG 
AGCTCCTCGC CTGTCCCTGT TGGTGGCAGC AGCTACAACA GCCCTGACTA CTCCCTCCAA 
CCATTCAGCT CTTCTCCAGA AAGCCAAGGC CAGCCACAGT ACAGTGCGGC CAGTGTCCAC 
ATGGTACCTG GGAGCCCTCA GGCACGACAC AGGACAGTGG GTACCAACAC TCCACCTAGC 
CCTGGTTTTG GCCGGCGGGC CGTCAACCCC ACCATGGCTG CCCCTGGTAG TCCCAGTTTG 
AGCCATCGGC AGGTGATGGG TCCATCTGGT CCAGGTTTCC ATGGTAACGT GGTTTCTGGC 
CACCCAGCCA GTGCAGCTAC CACTCCTGGA AGCCCCAGCC TGGGCCGGCA CCCAGTGGGA 
AGCCATCAAG TTCCAGGCCT CCACAGCAGT GTGGTCACCA CTCCGGGAAG CCCAAGCCTG 
GGCCGACACC CTGGTGCCCA CCAAGGGAAT CTGGCCTCCA GTCTCCACAG CAATGCAGTC 
ATCAGCCCTG GGAGCCCAAG CTTGGGCCGG CACCTGGGTG GGTCTGGATC CGTGGTTCCT 
GGAAGCCCCA GCTTGGACCG GCATGCAGCT TATGGTGGCT ACTCTACCCC TGAGGACCGA 
AGACCAACAC TGTCCCGGCA AAGCAGTGCC TCTGGCTACC AGGCCCCATC CACACCGTCA 
TTCCCTGTCT CTCCTGCCTA CTACCCTGGC CTGAGCAGCC CTGCCACCTC ACCCTCGCCA 
GACTCAGCAG CCTTCCGGCA GGGGAGCCCC ACACCAGCCT TACCAGAGAA GCGAAGAATG 
TCAGTGGGAG ACCGGGCAGG CAGCCTCCCC AACTATGCCA CCATCAATGG GAAAGTGTCT 
TCCTCGCCTG TCGCCAATGG CATGGCCAGT GGGAGCAGCA CCGTCTCCTT CTCTCACACG 
CTTCCGGACT TTTCTAAGTA TTCGATGCCA GACAATAGTC CGGAGACTCG GGCTAAAGTG 
AAGTTTGTCC AGGACACTTC CAAGTATTGG TACAAGCCTG AGATCTCCAG AGAACAGGCC 
ATCGCACTCC TAAAGGACCA GGAGCCAGGA GCCTTCATCA TCCGGGACAG CCACTCCTTC 
CGAGGGGCTT ATGGGCTGGC CATGAAGGTG TCCTCACCCC CTCCAACCAT CACTCAGCAG 
GGCAAGAAAG GAGACATGAC CCATGAGCTG GTGAGGCACT TTCTGATCGA GACAGGCCCT 
AGAGGAGTGA AGCTCAAGGG TTGTCCCAAT GAGCCAAACT TTGGTTCCCT TTCTGCCCTG 
GTCTATCAGC ACTCCGTCAT CCCACTGGCC CTGCCCTGTA AACTGGTCAT TCCAAGCCGA 
GACCCCACAG ATGAATCAAA AGATAGCTCA GGCCCTGCCA ACTCAACCAC TGACCTGCTG 
AAGCAAGGAG CAGCTTGCAA TGTGCTCTTC GTCAACTCTG TGGATATGGA GTCACTCACC 
GGGCCACAGG CCATTTCCAA AGCCACATCT GAGACTCTGG CTGCCGACCC CACACCGGCT 
GCCACCATTG TTCACTTCAA GGTCTCTGCC CAAGGAATCA CACTGACTGA CAATCAAAGA 
AAGCTCTTCT TTAGAAGACA CTACCCCCTC AACACTGTCA CCTTCTGTGA CCTGGATCCA 
CAAGAAAGAA AGTGGATGAA GACAGAGGGA GGCGCCCCTG CTAAGCTCTT TGGCTTCGTG 
GCCCGGAAGC AGGGCAGCAC CACAGACAAC GCTTGCCACC TCTTCGCAGA GCTTGACCCC 
AACCAGCCTG CGTCTGCCAT CGTCAACTTC GTCTCCAAGG TCATGCTGAG TGCTGGCCAG 
AAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495873.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495936.2 Mus musculus tensin 1 (Tns1), transcript variant X16, mRNA.

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CloneID	OMu68936
Clone ID Related Accession (Same CDS sequence)	XM_006495873.2
Accession Version	XM_006495873.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006495873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGCT CAGCCGACTT CATCTTGACG CTTTCCGGGG TGGCTGCCCC TTGCGTTCCT 
CCGTCCAGCC ATGAACTGGT GCCCATCACC ACAGAGACCG TACCCAAGAA TGTAGTAGAT 
GTGGGGGAAG GAGACTGCCG GGTTGGAAGT TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC 
ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495936.2
CDS	16..5502
Translation	MRSSADFILTLSGVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495873.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495873.2

ORF Insert Sequence:

ATGAGAAGCT CAGCCGACTT CATCTTGACG CTTTCCGGGG TGGCTGCCCC TTGCGTTCCT 
CCGTCCAGCC ATGAACTGGT GCCCATCACC ACAGAGACCG TACCCAAGAA TGTAGTAGAT 
GTGGGGGAAG GAGACTGCCG GGTTGGAAGT TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC 
ATGAGAGTTT CTCCAAGCAT ACAGCCACAA CCACAATCAC AGCCTACCAG TCTCTCTAGA 
AACACGAGTG TGAGCCGAGC CATGGAAGAC AGTTGTGAGC TGGACCTGGT GTATGTCACC 
GAACGCATCA TCGCCGTGTC CTTCCCTAGC ACAGCCAATG AGGAGAACTT CCGCAGCAAC 
CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC AAGCATGGGG GCAACTACCT GCTATTCAAC 
CTCTCTGAAC AGAGGCCTGA CATCACCAAG CTGCATGCTA AGGTCCTGGA ATTCGGCTGG 
CCTGACCTGC ACACACCGGC CCTGGAGAAG ATATGCAGTG TCTGTAAGGC CATGGACACC 
TGGCTCAATG CAGACCCCCA CAATGTCGTT GTTCTCCACA ACAAGGGAAA CCGAGGCAGG 
ATTGGGGTTG TCATTGCAGC GTATCTGCAC TACAGCAACA TTTCAGCCAG TGCTGACCAG 
GCTCTGGACC GGTTTGCAAT GAAGAGATTC TATGAGGACA AGATCGTGCC CATTGGCCAG 
CCATCCCAGA GGAGATATGT GCACTACTTC AGTGGCCTAC TCTCTGGCTC CATCAAAATG 
AACAACAAGC CTTTGTTTCT GCATCATGTG ATCATGCACG GCATCCCCAA CTTTGAGTCT 
AAAGGAGGGT GCCGGCCCTT TCTCCGCATC TACCAAGCCA TGCAACCTGT GTACACATCT 
GGCATTTACA ACATCCCTGG AGACAGCCAG GCCAGCATCT GTATCACCAT TGAACCAGGG 
CTGCTCTTGA AGGGAGACAT CTTGCTGAAG TGTTATCATA AGAAATTCCG GAGCCCGGCT 
CGAGACGTCA TCTTCCGAGT GCAGTTCCAC ACCTGTGCCA TCCATGACTT GGGGGTTGTT 
TTTGGGAAGG AAGATCTGGA TGAGGCCTTC AAAGATGATC GATTTCCTGA CTATGGCAAA 
GTGGAGTTTG TCTTTTCCTA TGGACCAGAG AAAATTCAAG GCATGGAACA CCTGGAGAAT 
GGGCCCAGCG TATCTGTAGA CTATAACACT TCTGACCCCC TCATCCGCTG GGACTCCTAT 
GACAACTTCA GTGGGCATCG TGAGGATGGC ATGGAAGGTT TAAAAGTGGT GGGACACACC 
CAAGGGCCGC TGGATGGGAG CCTGTACGCC AAGGTGAAGA AGAAGGATTC CCTGAATGGC 
AGTTCGGGGC CTGTCACTAC TGCACGGCCT GCCCTGTCAG CCACTCCCAA CCACGTGGAA 
CACACCCTGT CTGTGAGCAG CGACTCTGGC AACTCCACAG CCTCCACTAA GACAGACAAG 
ACCGATGAGC CAGTCTCTGG GGCTACCACT GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG 
AAAGAGCTGG ACCGCCTACT AAGTGGCTTT GGTGTGGATA GAGAAAAGCA AGGCGCCATG 
TACCGGGCTC AGCAACTCAG ATCCCATCCA GGCGGGGGCC CGACTGTGCC CTCCCCTGGC 
CGCCACATTG TCCCAGCCCA GGTTCACGTC AATGGTGGGG CCCTGGCATC TGAGCGGGAG 
ACAGACATTC TAGATGATGA GCTGCCCATC CAGGACGGGC AGAGTGGGGG CAGCATGGGA 
ACGCTGTCCT CCCTGGATGG GGTCACCAAC ACCAGTGAAA GTGGCTACCC AGAGACCCTG 
TCCCCACTGA CCAATGGCCT GGACAAACCC TATTCCACAG AACCTGTGCT CAACGGCGGA 
GGCTACCCCT ATGAGGCTGC CAACCGGGTG ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC 
ATTCGACCCT CCTACTCTGC TCAGGAGGGT TTAGCTGGCT ACCAGCGTGA GGGACCCCAC 
CCAGCCTGGT CACAGCAAGT GACCAGTGCC CACTGTGGCT GTGACCCCAG TGGTCTATTC 
AGATCCCAGT CCTTTCCAGA TGTGGAGCCC CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT 
AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG AATTCATGGC AACAGCAGCA GCCCCACCCA 
CCTCCCCGCC AGCAGGAGAG ATCCCCACTG CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC 
CAGCTGTCAG CAGAGACCCC CGTAGCTGCT CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG 
CAGGAGATCG AGCAGTCCAT CGAAACCCTC AATATGTTGA TGCTGGACCT GGAGCCAGCC 
TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT 
CCACTCTCTT CCCAGCCCCT CTTGGGGTCT TCCCGCCAGT CCCACCCACT TACCCAGTCC 
CGATCTGGCT ATATCCCCAG TGGGCATTCT CTGGGAACCC CGGAGCTGGT CTCTTCTGGC 
AGGCCTTATT CACCTTACGA TTATCAGCTG CATCCAGCTG GATCCAACCA GAGTTTCCAT 
CCAAAAAGTC CAGCCTCTTC CACCTTCCTT CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG 
GAGCCCCCAG CCTCTCTCCC TGGTCTCATC GCTCAGCCCC AGCTCCCACC AAAGGAAACT 
ACATCAGATC CCTCCCGAAC TCCAGAGGAA GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC 
CATCGGGTAG CAGTGTACAA TGCCAGACTA CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA 
CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( NM_027884.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082160.3 Mus musculus tensin 1 (Tns1), transcript variant 1, mRNA.

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CloneID	OMu04724
Clone ID Related Accession (Same CDS sequence)	NM_027884.3 , NM_027884.4
Accession Version	NM_027884.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-11
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC117757.23 and AC116419.32. On or before Apr 9, 2009 this sequence version replaced XM_984786.1, XM_619639.3, NM_027884.2. Transcript Variant: This variant represents the longer transcript and encodes the longer isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence because no single transcript was available for the full length of the gene. The exon combination of this transcript is inferred based on partial mouse and orthologous transcript alignments, and on RNA-seq data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGCA CCGTGAGCCT GGTGTGCTGC GAGGCGCTCG AGCCTCTGCC CTCCTGCGGC 
CCGCAGCCCC CCGGGACTCC GCCGGGCCCC GCGCGGCCCG AGCGCTGTGA GCCCGGGGGT 
GCGGCGCCCG ACCCGAGGAG GCGGCTGCTG CTTCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGAAG TGGTGGGACA CACCCAAGGG CCGCTGGATG GGAGCCTGTA CGCCAAGGTG 
AAGAAGAAGG ATTCCCTGAA TGGCAGTTCG GGGCCTGTCA CTACTGCACG GCCTGCCCTG 
TCAGCCACTC CCAACCACGT GGAACACACC CTGTCTGTGA GCAGCGACTC TGGCAACTCC 
ACAGCCTCCA CTAAGACAGA CAAGACCGAT GAGCCAGTCT CTGGGGCTAC CACTGCCCCT 
GCTGCCCTGA GTCCTCAGGA GAAGAAAGAG CTGGACCGCC TACTAAGTGG CTTTGGTGTG 
GATAGAGAAA AGCAAGGCGC CATGTACCGG GCTCAGCAAC TCAGATCCCA TCCAGGCGGG 
GGCCCGACTG TGCCCTCCCC TGGCCGCCAC ATTGTCCCAG CCCAGGTTCA CGTCAATGGT 
GGGGCCCTGG CATCTGAGCG GGAGACAGAC ATTCTAGATG ATGAGCTGCC CATCCAGGAC 
GGGCAGAGTG GGGGCAGCAT GGGAACGCTG TCCTCCCTGG ATGGGGTCAC CAACACCAGT 
GAAAGTGGCT ACCCAGAGAC CCTGTCCCCA CTGACCAATG GCCTGGACAA ACCCTATTCC 
ACAGAACCTG TGCTCAACGG CGGAGGCTAC CCCTATGAGG CTGCCAACCG GGTGATTCCT 
GTCCACAGCA GTCACTCGGC CCCCATTCGA CCCTCCTACT CTGCTCAGGA GGGTTTAGCT 
GGCTACCAGC GTGAGGGACC CCACCCAGCC TGGTCACAGC AAGTGACCAG TGCCCACTGT 
GGCTGTGACC CCAGTGGTCT ATTCAGATCC CAGTCCTTTC CAGATGTGGA GCCCCAGCTG 
CCCCAAGCTC CAACCCGTGG GGGTAGTAGC CGAGAGGCTG TGCAGAGGGG TCTGAATTCA 
TGGCAACAGC AGCAGCCCCA CCCACCTCCC CGCCAGCAGG AGAGATCCCC ACTGCAGAGT 
CTTGCCCGCA GCAAGCCTAG CCCCCAGCTG TCAGCAGAGA CCCCCGTAGC TGCTCTCCCA 
GAGTTCCCAA GGGCAGCGTC CCAGCAGGAG ATCGAGCAGT CCATCGAAAC CCTCAATATG 
TTGATGCTGG ACCTGGAGCC AGCCTCCGCA GCTGCCCCTC TTCATAAGTC CCAGAGTGTC 
CCTGGGGCCT GGCCAGGGGC TTCTCCACTC TCTTCCCAGC CCCTCTTGGG GTCTTCCCGC 
CAGTCCCACC CACTTACCCA GTCCCGATCT GGCTATATCC CCAGTGGGCA TTCTCTGGGA 
ACCCCGGAGC TGGTCTCTTC TGGCAGGCCT TATTCACCTT ACGATTATCA GCTGCATCCA 
GCTGGATCCA ACCAGAGTTT CCATCCAAAA AGTCCAGCCT CTTCCACCTT CCTTCCAAGC 
CCTCATAGCT CTGCGGGGCC TCAGGAGCCC CCAGCCTCTC TCCCTGGTCT CATCGCTCAG 
CCCCAGCTCC CACCAAAGGA AACTACATCA GATCCCTCCC GAACTCCAGA GGAAGAGCCG 
CTGAACCTGG AAGGGCTGGT AGCCCATCGG GTAGCAGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082160.3
CDS	1..5667
Translation	MGCTVSLVCCEALEPLPSCGPQPPGTPPGPARPERCEPGGAAPDPRRRLLLQPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEEVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	NM_027884.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027884.3

ORF Insert Sequence:

ATGGGCTGCA CCGTGAGCCT GGTGTGCTGC GAGGCGCTCG AGCCTCTGCC CTCCTGCGGC 
CCGCAGCCCC CCGGGACTCC GCCGGGCCCC GCGCGGCCCG AGCGCTGTGA GCCCGGGGGT 
GCGGCGCCCG ACCCGAGGAG GCGGCTGCTG CTTCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGAAG TGGTGGGACA CACCCAAGGG CCGCTGGATG GGAGCCTGTA CGCCAAGGTG 
AAGAAGAAGG ATTCCCTGAA TGGCAGTTCG GGGCCTGTCA CTACTGCACG GCCTGCCCTG 
TCAGCCACTC CCAACCACGT GGAACACACC CTGTCTGTGA GCAGCGACTC TGGCAACTCC 
ACAGCCTCCA CTAAGACAGA CAAGACCGAT GAGCCAGTCT CTGGGGCTAC CACTGCCCCT 
GCTGCCCTGA GTCCTCAGGA GAAGAAAGAG CTGGACCGCC TACTAAGTGG CTTTGGTGTG 
GATAGAGAAA AGCAAGGCGC CATGTACCGG GCTCAGCAAC TCAGATCCCA TCCAGGCGGG 
GGCCCGACTG TGCCCTCCCC TGGCCGCCAC ATTGTCCCAG CCCAGGTTCA CGTCAATGGT 
GGGGCCCTGG CATCTGAGCG GGAGACAGAC ATTCTAGATG ATGAGCTGCC CATCCAGGAC 
GGGCAGAGTG GGGGCAGCAT GGGAACGCTG TCCTCCCTGG ATGGGGTCAC CAACACCAGT 
GAAAGTGGCT ACCCAGAGAC CCTGTCCCCA CTGACCAATG GCCTGGACAA ACCCTATTCC 
ACAGAACCTG TGCTCAACGG CGGAGGCTAC CCCTATGAGG CTGCCAACCG GGTGATTCCT 
GTCCACAGCA GTCACTCGGC CCCCATTCGA CCCTCCTACT CTGCTCAGGA GGGTTTAGCT 
GGCTACCAGC GTGAGGGACC CCACCCAGCC TGGTCACAGC AAGTGACCAG TGCCCACTGT 
GGCTGTGACC CCAGTGGTCT ATTCAGATCC CAGTCCTTTC CAGATGTGGA GCCCCAGCTG 
CCCCAAGCTC CAACCCGTGG GGGTAGTAGC CGAGAGGCTG TGCAGAGGGG TCTGAATTCA 
TGGCAACAGC AGCAGCCCCA CCCACCTCCC CGCCAGCAGG AGAGATCCCC ACTGCAGAGT 
CTTGCCCGCA GCAAGCCTAG CCCCCAGCTG TCAGCAGAGA CCCCCGTAGC TGCTCTCCCA 
GAGTTCCCAA GGGCAGCGTC CCAGCAGGAG ATCGAGCAGT CCATCGAAAC CCTCAATATG 
TTGATGCTGG ACCTGGAGCC AGCCTCCGCA GCTGCCCCTC TTCATAAGTC CCAGAGTGTC 
CCTGGGGCCT GGCCAGGGGC TTCTCCACTC TCTTCCCAGC CCCTCTTGGG GTCTTCCCGC 
CAGTCCCACC CACTTACCCA GTCCCGATCT GGCTATATCC CCAGTGGGCA TTCTCTGGGA 
ACCCCGGAGC TGGTCTCTTC TGGCAGGCCT TATTCACCTT ACGATTATCA GCTGCATCCA 
GCTGGATCCA ACCAGAGTTT CCATCCAAAA AGTCCAGCCT CTTCCACCTT CCTTCCAAGC 
CCTCATAGCT CTGCGGGGCC TCAGGAGCCC CCAGCCTCTC TCCCTGGTCT CATCGCTCAG 
CCCCAGCTCC CACCAAAGGA AACTACATCA GATCCCTCCC GAACTCCAGA GGAAGAGCCG 
CTGAACCTGG AAGGGCTGGT AGCCCATCGG GTAGCAGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( NM_027884.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082160.3 Mus musculus tensin 1 (Tns1), transcript variant 1, mRNA.

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CloneID	OMu04724
Clone ID Related Accession (Same CDS sequence)	NM_027884.3 , NM_027884.4
Accession Version	NM_027884.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC117757.23 and AC116419.32. On Jun 18, 2019 this sequence version replaced NM_027884.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGCA CCGTGAGCCT GGTGTGCTGC GAGGCGCTCG AGCCTCTGCC CTCCTGCGGC 
CCGCAGCCCC CCGGGACTCC GCCGGGCCCC GCGCGGCCCG AGCGCTGTGA GCCCGGGGGT 
GCGGCGCCCG ACCCGAGGAG GCGGCTGCTG CTTCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGAAG TGGTGGGACA CACCCAAGGG CCGCTGGATG GGAGCCTGTA CGCCAAGGTG 
AAGAAGAAGG ATTCCCTGAA TGGCAGTTCG GGGCCTGTCA CTACTGCACG GCCTGCCCTG 
TCAGCCACTC CCAACCACGT GGAACACACC CTGTCTGTGA GCAGCGACTC TGGCAACTCC 
ACAGCCTCCA CTAAGACAGA CAAGACCGAT GAGCCAGTCT CTGGGGCTAC CACTGCCCCT 
GCTGCCCTGA GTCCTCAGGA GAAGAAAGAG CTGGACCGCC TACTAAGTGG CTTTGGTGTG 
GATAGAGAAA AGCAAGGCGC CATGTACCGG GCTCAGCAAC TCAGATCCCA TCCAGGCGGG 
GGCCCGACTG TGCCCTCCCC TGGCCGCCAC ATTGTCCCAG CCCAGGTTCA CGTCAATGGT 
GGGGCCCTGG CATCTGAGCG GGAGACAGAC ATTCTAGATG ATGAGCTGCC CATCCAGGAC 
GGGCAGAGTG GGGGCAGCAT GGGAACGCTG TCCTCCCTGG ATGGGGTCAC CAACACCAGT 
GAAAGTGGCT ACCCAGAGAC CCTGTCCCCA CTGACCAATG GCCTGGACAA ACCCTATTCC 
ACAGAACCTG TGCTCAACGG CGGAGGCTAC CCCTATGAGG CTGCCAACCG GGTGATTCCT 
GTCCACAGCA GTCACTCGGC CCCCATTCGA CCCTCCTACT CTGCTCAGGA GGGTTTAGCT 
GGCTACCAGC GTGAGGGACC CCACCCAGCC TGGTCACAGC AAGTGACCAG TGCCCACTGT 
GGCTGTGACC CCAGTGGTCT ATTCAGATCC CAGTCCTTTC CAGATGTGGA GCCCCAGCTG 
CCCCAAGCTC CAACCCGTGG GGGTAGTAGC CGAGAGGCTG TGCAGAGGGG TCTGAATTCA 
TGGCAACAGC AGCAGCCCCA CCCACCTCCC CGCCAGCAGG AGAGATCCCC ACTGCAGAGT 
CTTGCCCGCA GCAAGCCTAG CCCCCAGCTG TCAGCAGAGA CCCCCGTAGC TGCTCTCCCA 
GAGTTCCCAA GGGCAGCGTC CCAGCAGGAG ATCGAGCAGT CCATCGAAAC CCTCAATATG 
TTGATGCTGG ACCTGGAGCC AGCCTCCGCA GCTGCCCCTC TTCATAAGTC CCAGAGTGTC 
CCTGGGGCCT GGCCAGGGGC TTCTCCACTC TCTTCCCAGC CCCTCTTGGG GTCTTCCCGC 
CAGTCCCACC CACTTACCCA GTCCCGATCT GGCTATATCC CCAGTGGGCA TTCTCTGGGA 
ACCCCGGAGC TGGTCTCTTC TGGCAGGCCT TATTCACCTT ACGATTATCA GCTGCATCCA 
GCTGGATCCA ACCAGAGTTT CCATCCAAAA AGTCCAGCCT CTTCCACCTT CCTTCCAAGC 
CCTCATAGCT CTGCGGGGCC TCAGGAGCCC CCAGCCTCTC TCCCTGGTCT CATCGCTCAG 
CCCCAGCTCC CACCAAAGGA AACTACATCA GATCCCTCCC GAACTCCAGA GGAAGAGCCG 
CTGAACCTGG AAGGGCTGGT AGCCCATCGG GTAGCAGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082160.3
CDS	102..5768
Translation	MGCTVSLVCCEALEPLPSCGPQPPGTPPGPARPERCEPGGAAPDPRRRLLLQPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEEVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	NM_027884.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027884.4

ORF Insert Sequence:

ATGGGCTGCA CCGTGAGCCT GGTGTGCTGC GAGGCGCTCG AGCCTCTGCC CTCCTGCGGC 
CCGCAGCCCC CCGGGACTCC GCCGGGCCCC GCGCGGCCCG AGCGCTGTGA GCCCGGGGGT 
GCGGCGCCCG ACCCGAGGAG GCGGCTGCTG CTTCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGAAG TGGTGGGACA CACCCAAGGG CCGCTGGATG GGAGCCTGTA CGCCAAGGTG 
AAGAAGAAGG ATTCCCTGAA TGGCAGTTCG GGGCCTGTCA CTACTGCACG GCCTGCCCTG 
TCAGCCACTC CCAACCACGT GGAACACACC CTGTCTGTGA GCAGCGACTC TGGCAACTCC 
ACAGCCTCCA CTAAGACAGA CAAGACCGAT GAGCCAGTCT CTGGGGCTAC CACTGCCCCT 
GCTGCCCTGA GTCCTCAGGA GAAGAAAGAG CTGGACCGCC TACTAAGTGG CTTTGGTGTG 
GATAGAGAAA AGCAAGGCGC CATGTACCGG GCTCAGCAAC TCAGATCCCA TCCAGGCGGG 
GGCCCGACTG TGCCCTCCCC TGGCCGCCAC ATTGTCCCAG CCCAGGTTCA CGTCAATGGT 
GGGGCCCTGG CATCTGAGCG GGAGACAGAC ATTCTAGATG ATGAGCTGCC CATCCAGGAC 
GGGCAGAGTG GGGGCAGCAT GGGAACGCTG TCCTCCCTGG ATGGGGTCAC CAACACCAGT 
GAAAGTGGCT ACCCAGAGAC CCTGTCCCCA CTGACCAATG GCCTGGACAA ACCCTATTCC 
ACAGAACCTG TGCTCAACGG CGGAGGCTAC CCCTATGAGG CTGCCAACCG GGTGATTCCT 
GTCCACAGCA GTCACTCGGC CCCCATTCGA CCCTCCTACT CTGCTCAGGA GGGTTTAGCT 
GGCTACCAGC GTGAGGGACC CCACCCAGCC TGGTCACAGC AAGTGACCAG TGCCCACTGT 
GGCTGTGACC CCAGTGGTCT ATTCAGATCC CAGTCCTTTC CAGATGTGGA GCCCCAGCTG 
CCCCAAGCTC CAACCCGTGG GGGTAGTAGC CGAGAGGCTG TGCAGAGGGG TCTGAATTCA 
TGGCAACAGC AGCAGCCCCA CCCACCTCCC CGCCAGCAGG AGAGATCCCC ACTGCAGAGT 
CTTGCCCGCA GCAAGCCTAG CCCCCAGCTG TCAGCAGAGA CCCCCGTAGC TGCTCTCCCA 
GAGTTCCCAA GGGCAGCGTC CCAGCAGGAG ATCGAGCAGT CCATCGAAAC CCTCAATATG 
TTGATGCTGG ACCTGGAGCC AGCCTCCGCA GCTGCCCCTC TTCATAAGTC CCAGAGTGTC 
CCTGGGGCCT GGCCAGGGGC TTCTCCACTC TCTTCCCAGC CCCTCTTGGG GTCTTCCCGC 
CAGTCCCACC CACTTACCCA GTCCCGATCT GGCTATATCC CCAGTGGGCA TTCTCTGGGA 
ACCCCGGAGC TGGTCTCTTC TGGCAGGCCT TATTCACCTT ACGATTATCA GCTGCATCCA 
GCTGGATCCA ACCAGAGTTT CCATCCAAAA AGTCCAGCCT CTTCCACCTT CCTTCCAAGC 
CCTCATAGCT CTGCGGGGCC TCAGGAGCCC CCAGCCTCTC TCCCTGGTCT CATCGCTCAG 
CCCCAGCTCC CACCAAAGGA AACTACATCA GATCCCTCCC GAACTCCAGA GGAAGAGCCG 
CTGAACCTGG AAGGGCTGGT AGCCCATCGG GTAGCAGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGTTGCCAG AAGACTGATC 
CAGCCAGAGG AAGACGAGGG GGAGGAGGTG ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT 
GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA GGGCCTCGAG CTCAGGACGT TGAGCCTAAG 
AGCTTCAGCG CACCAGCTGC TCACGCCTAT GGGCACGAGA CACCCCTGAG GAACGGGACC 
CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC CTCTCCACAA GCAGCCCCAT CCTCAGTGCT 
GACAGCACTT CCGTGGGCAG TTTCCCATCA GTGGTGAGCA GTGACCAGGG TCCCCGGACA 
CCCTTCCAGC CTATGCTGGA CTCCAGCATC CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT 
GCAGCACTGA GTTATCAGAG CTCCTCGCCT GTCCCTGTTG GTGGCAGCAG CTACAACAGC 
CCTGACTACT CCCTCCAACC ATTCAGCTCT TCTCCAGAAA GCCAAGGCCA GCCACAGTAC 
AGTGCGGCCA GTGTCCACAT GGTACCTGGG AGCCCTCAGG CACGACACAG GACAGTGGGT 
ACCAACACTC CACCTAGCCC TGGTTTTGGC CGGCGGGCCG TCAACCCCAC CATGGCTGCC 
CCTGGTAGTC CCAGTTTGAG CCATCGGCAG GTGATGGGTC CATCTGGTCC AGGTTTCCAT 
GGTAACGTGG TTTCTGGCCA CCCAGCCAGT GCAGCTACCA CTCCTGGAAG CCCCAGCCTG 
GGCCGGCACC CAGTGGGAAG CCATCAAGTT CCAGGCCTCC ACAGCAGTGT GGTCACCACT 
CCGGGAAGCC CAAGCCTGGG CCGACACCCT GGTGCCCACC AAGGGAATCT GGCCTCCAGT 
CTCCACAGCA ATGCAGTCAT CAGCCCTGGG AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG 
TCTGGATCCG TGGTTCCTGG AAGCCCCAGC TTGGACCGGC ATGCAGCTTA TGGTGGCTAC 
TCTACCCCTG AGGACCGAAG ACCAACACTG TCCCGGCAAA GCAGTGCCTC TGGCTACCAG 
GCCCCATCCA CACCGTCATT CCCTGTCTCT CCTGCCTACT ACCCTGGCCT GAGCAGCCCT 
GCCACCTCAC CCTCGCCAGA CTCAGCAGCC TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA 
CCAGAGAAGC GAAGAATGTC AGTGGGAGAC CGGGCAGGCA GCCTCCCCAA CTATGCCACC 
ATCAATGGGA AAGTGTCTTC CTCGCCTGTC GCCAATGGCA TGGCCAGTGG GAGCAGCACC 
GTCTCCTTCT CTCACACGCT TCCGGACTTT TCTAAGTATT CGATGCCAGA CAATAGTCCG 
GAGACTCGGG CTAAAGTGAA GTTTGTCCAG GACACTTCCA AGTATTGGTA CAAGCCTGAG 
ATCTCCAGAG AACAGGCCAT CGCACTCCTA AAGGACCAGG AGCCAGGAGC CTTCATCATC 
CGGGACAGCC ACTCCTTCCG AGGGGCTTAT GGGCTGGCCA TGAAGGTGTC CTCACCCCCT 
CCAACCATCA CTCAGCAGGG CAAGAAAGGA GACATGACCC ATGAGCTGGT GAGGCACTTT 
CTGATCGAGA CAGGCCCTAG AGGAGTGAAG CTCAAGGGTT GTCCCAATGA GCCAAACTTT 
GGTTCCCTTT CTGCCCTGGT CTATCAGCAC TCCGTCATCC CACTGGCCCT GCCCTGTAAA 
CTGGTCATTC CAAGCCGAGA CCCCACAGAT GAATCAAAAG ATAGCTCAGG CCCTGCCAAC 
TCAACCACTG ACCTGCTGAA GCAAGGAGCA GCTTGCAATG TGCTCTTCGT CAACTCTGTG 
GATATGGAGT CACTCACCGG GCCACAGGCC ATTTCCAAAG CCACATCTGA GACTCTGGCT 
GCCGACCCCA CACCGGCTGC CACCATTGTT CACTTCAAGG TCTCTGCCCA AGGAATCACA 
CTGACTGACA ATCAAAGAAA GCTCTTCTTT AGAAGACACT ACCCCCTCAA CACTGTCACC 
TTCTGTGACC TGGATCCACA AGAAAGAAAG TGGATGAAGA CAGAGGGAGG CGCCCCTGCT 
AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG GGCAGCACCA CAGACAACGC TTGCCACCTC 
TTCGCAGAGC TTGACCCCAA CCAGCCTGCG TCTGCCATCG TCAACTTCGT CTCCAAGGTC 
ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( NM_001289895.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276824.1 Mus musculus tensin 1 (Tns1), transcript variant 2, mRNA.

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CloneID	OMu58480
Clone ID Related Accession (Same CDS sequence)	NM_001289895.1 , NM_001289895.2
Accession Version	NM_001289895.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-11
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ125619.1, AI315947.1, AK053112.1, AK137923.1, AC116419.32, CF540284.1, AW611438.1, CN697610.1, CF171717.1, BC052740.1, AK004758.1 and CJ241954.1. Transcript Variant: This variant (2) lacks two in-frame exons in the 3' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The exon combination of this transcript is inferred based on partial mouse and orthologous transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGCA CCGTGAGCCT GGTGTGCTGC GAGGCGCTCG AGCCTCTGCC CTCCTGCGGC 
CCGCAGCCCC CCGGGACTCC GCCGGGCCCC GCGCGGCCCG AGCGCTGTGA GCCCGGGGGT 
GCGGCGCCCG ACCCGAGGAG GCGGCTGCTG CTTCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGAAG TGGTGGGACA CACCCAAGGG CCGCTGGATG GGAGCCTGTA CGCCAAGGTG 
AAGAAGAAGG ATTCCCTGAA TGGCAGTTCG GGGCCTGTCA CTACTGCACG GCCTGCCCTG 
TCAGCCACTC CCAACCACGT GGAACACACC CTGTCTGTGA GCAGCGACTC TGGCAACTCC 
ACAGCCTCCA CTAAGACAGA CAAGACCGAT GAGCCAGTCT CTGGGGCTAC CACTGCCCCT 
GCTGCCCTGA GTCCTCAGGA GAAGAAAGAG CTGGACCGCC TACTAAGTGG CTTTGGTGTG 
GATAGAGAAA AGCAAGGCGC CATGTACCGG GCTCAGCAAC TCAGATCCCA TCCAGGCGGG 
GGCCCGACTG TGCCCTCCCC TGGCCGCCAC ATTGTCCCAG CCCAGGTTCA CGTCAATGGT 
GGGGCCCTGG CATCTGAGCG GGAGACAGAC ATTCTAGATG ATGAGCTGCC CATCCAGGAC 
GGGCAGAGTG GGGGCAGCAT GGGAACGCTG TCCTCCCTGG ATGGGGTCAC CAACACCAGT 
GAAAGTGGCT ACCCAGAGAC CCTGTCCCCA CTGACCAATG GCCTGGACAA ACCCTATTCC 
ACAGAACCTG TGCTCAACGG CGGAGGCTAC CCCTATGAGG CTGCCAACCG GGTGATTCCT 
GTCCACAGCA GTCACTCGGC CCCCATTCGA CCCTCCTACT CTGCTCAGGA GGGTTTAGCT 
GGCTACCAGC GTGAGGGACC CCACCCAGCC TGGTCACAGC AAGTGACCAG TGCCCACTGT 
GGCTGTGACC CCAGTGGTCT ATTCAGATCC CAGTCCTTTC CAGATGTGGA GCCCCAGCTG 
CCCCAAGCTC CAACCCGTGG GGGTAGTAGC CGAGAGGCTG TGCAGAGGGG TCTGAATTCA 
TGGCAACAGC AGCAGCCCCA CCCACCTCCC CGCCAGCAGG AGAGATCCCC ACTGCAGAGT 
CTTGCCCGCA GCAAGCCTAG CCCCCAGCTG TCAGCAGAGA CCCCCGTAGC TGCTCTCCCA 
GAGTTCCCAA GGGCAGCGTC CCAGCAGGAG ATCGAGCAGT CCATCGAAAC CCTCAATATG 
TTGATGCTGG ACCTGGAGCC AGCCTCCGCA GCTGCCCCTC TTCATAAGTC CCAGAGTGTC 
CCTGGGGCCT GGCCAGGGGC TTCTCCACTC TCTTCCCAGC CCCTCTTGGG GTCTTCCCGC 
CAGTCCCACC CACTTACCCA GTCCCGATCT GGCTATATCC CCAGTGGGCA TTCTCTGGGA 
ACCCCGGAGC TGGTCTCTTC TGGCAGGCCT TATTCACCTT ACGATTATCA GCTGCATCCA 
GCTGGATCCA ACCAGAGTTT CCATCCAAAA AGTCCAGCCT CTTCCACCTT CCTTCCAAGC 
CCTCATAGCT CTGCGGGGCC TCAGGAGCCC CCAGCCTCTC TCCCTGGTCT CATCGCTCAG 
CCCCAGCTCC CACCAAAGGA AACTACATCA GATCCCTCCC GAACTCCAGA GGAAGAGCCG 
CTGAACCTGG AAGGGCTGGT AGCCCATCGG GTAGCAGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGAGCCCCG GAGCTATGTG 
GAGTCCGTGG CGAGGACAGC GGTGGCAGGG CCTCGAGCTC AGGACGTTGA GCCTAAGAGC 
TTCAGCGCAC CAGCTGCTCA CGCCTATGGG CACGAGACAC CCCTGAGGAA CGGGACCCCA 
GGGGGCTCCT TTGTCTCCCC GAGCCCCCTC TCCACAAGCA GCCCCATCCT CAGTGCTGAC 
AGCACTTCCG TGGGCAGTTT CCCATCAGTG GTGAGCAGTG ACCAGGGTCC CCGGACACCC 
TTCCAGCCTA TGCTGGACTC CAGCATCCGC TCAGGCAGCT TGGGGCAGCC GAGCCCTGCA 
GCACTGAGTT ATCAGAGCTC CTCGCCTGTC CCTGTTGGTG GCAGCAGCTA CAACAGCCCT 
GACTACTCCC TCCAACCATT CAGCTCTTCT CCAGAAAGCC AAGGCCAGCC ACAGTACAGT 
GCGGCCAGTG TCCACATGGT ACCTGGGAGC CCTCAGGCAC GACACAGGAC AGTGGGTACC 
AACACTCCAC CTAGCCCTGG TTTTGGCCGG CGGGCCGTCA ACCCCACCAT GGCTGCCCCT 
GGTAGTCCCA GTTTGAGCCA TCGGCAGGTG ATGGGTCCAT CTGGTCCAGG TTTCCATGGT 
AACGTGGTTT CTGGCCACCC AGCCAGTGCA GCTACCACTC CTGGAAGCCC CAGCCTGGGC 
CGGCACCCAG TGGGAAGCCA TCAAGTTCCA GGCCTCCACA GCAGTGTGGT CACCACTCCG 
GGAAGCCCAA GCCTGGGCCG ACACCCTGGT GCCCACCAAG GGAATCTGGC CTCCAGTCTC 
CACAGCAATG CAGTCATCAG CCCTGGGAGC CCAAGCTTGG GCCGGCACCT GGGTGGGTCT 
GGATCCGTGG TTCCTGGAAG CCCCAGCTTG GACCGGCATG CAGCTTATGG TGGCTACTCT 
ACCCCTGAGG ACCGAAGACC AACACTGTCC CGGCAAAGCA GTGCCTCTGG CTACCAGGCC 
CCATCCACAC CGTCATTCCC TGTCTCTCCT GCCTACTACC CTGGCCTGAG CAGCCCTGCC 
ACCTCACCCT CGCCAGACTC AGCAGCCTTC CGGCAGGGGA GCCCCACACC AGCCTTACCA 
GAGAAGCGAA GAATGTCAGT GGGAGACCGG GCAGGCAGCC TCCCCAACTA TGCCACCATC 
AATGGGAAAG TGTCTTCCTC GCCTGTCGCC AATGGCATGG CCAGTGGGAG CAGCACCGTC 
TCCTTCTCTC ACACGCTTCC GGACTTTTCT AAGTATTCGA TGCCAGACAA TAGTCCGGAG 
ACTCGGGCTA AAGTGAAGTT TGTCCAGGAC ACTTCCAAGT ATTGGTACAA GCCTGAGATC 
TCCAGAGAAC AGGCCATCGC ACTCCTAAAG GACCAGGAGC CAGGAGCCTT CATCATCCGG 
GACAGCCACT CCTTCCGAGG GGCTTATGGG CTGGCCATGA AGGTGTCCTC ACCCCCTCCA 
ACCATCACTC AGCAGGGCAA GAAAGGAGAC ATGACCCATG AGCTGGTGAG GCACTTTCTG 
ATCGAGACAG GCCCTAGAGG AGTGAAGCTC AAGGGTTGTC CCAATGAGCC AAACTTTGGT 
TCCCTTTCTG CCCTGGTCTA TCAGCACTCC GTCATCCCAC TGGCCCTGCC CTGTAAACTG 
GTCATTCCAA GCCGAGACCC CACAGATGAA TCAAAAGATA GCTCAGGCCC TGCCAACTCA 
ACCACTGACC TGCTGAAGCA AGGAGCAGCT TGCAATGTGC TCTTCGTCAA CTCTGTGGAT 
ATGGAGTCAC TCACCGGGCC ACAGGCCATT TCCAAAGCCA CATCTGAGAC TCTGGCTGCC 
GACCCCACAC CGGCTGCCAC CATTGTTCAC TTCAAGGTCT CTGCCCAAGG AATCACACTG 
ACTGACAATC AAAGAAAGCT CTTCTTTAGA AGACACTACC CCCTCAACAC TGTCACCTTC 
TGTGACCTGG ATCCACAAGA AAGAAAGTGG ATGAAGACAG AGGGAGGCGC CCCTGCTAAG 
CTCTTTGGCT TCGTGGCCCG GAAGCAGGGC AGCACCACAG ACAACGCTTG CCACCTCTTC 
GCAGAGCTTG ACCCCAACCA GCCTGCGTCT GCCATCGTCA ACTTCGTCTC CAAGGTCATG 
CTGAGTGCTG GCCAGAAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276824.1
CDS	3..5606
Translation	MGCTVSLVCCEALEPLPSCGPQPPGTPPGPARPERCEPGGAAPDPRRRLLLQPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEEVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	NM_001289895.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289895.1

ORF Insert Sequence:

ATGGGCTGCA CCGTGAGCCT GGTGTGCTGC GAGGCGCTCG AGCCTCTGCC CTCCTGCGGC 
CCGCAGCCCC CCGGGACTCC GCCGGGCCCC GCGCGGCCCG AGCGCTGTGA GCCCGGGGGT 
GCGGCGCCCG ACCCGAGGAG GCGGCTGCTG CTTCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGAAG TGGTGGGACA CACCCAAGGG CCGCTGGATG GGAGCCTGTA CGCCAAGGTG 
AAGAAGAAGG ATTCCCTGAA TGGCAGTTCG GGGCCTGTCA CTACTGCACG GCCTGCCCTG 
TCAGCCACTC CCAACCACGT GGAACACACC CTGTCTGTGA GCAGCGACTC TGGCAACTCC 
ACAGCCTCCA CTAAGACAGA CAAGACCGAT GAGCCAGTCT CTGGGGCTAC CACTGCCCCT 
GCTGCCCTGA GTCCTCAGGA GAAGAAAGAG CTGGACCGCC TACTAAGTGG CTTTGGTGTG 
GATAGAGAAA AGCAAGGCGC CATGTACCGG GCTCAGCAAC TCAGATCCCA TCCAGGCGGG 
GGCCCGACTG TGCCCTCCCC TGGCCGCCAC ATTGTCCCAG CCCAGGTTCA CGTCAATGGT 
GGGGCCCTGG CATCTGAGCG GGAGACAGAC ATTCTAGATG ATGAGCTGCC CATCCAGGAC 
GGGCAGAGTG GGGGCAGCAT GGGAACGCTG TCCTCCCTGG ATGGGGTCAC CAACACCAGT 
GAAAGTGGCT ACCCAGAGAC CCTGTCCCCA CTGACCAATG GCCTGGACAA ACCCTATTCC 
ACAGAACCTG TGCTCAACGG CGGAGGCTAC CCCTATGAGG CTGCCAACCG GGTGATTCCT 
GTCCACAGCA GTCACTCGGC CCCCATTCGA CCCTCCTACT CTGCTCAGGA GGGTTTAGCT 
GGCTACCAGC GTGAGGGACC CCACCCAGCC TGGTCACAGC AAGTGACCAG TGCCCACTGT 
GGCTGTGACC CCAGTGGTCT ATTCAGATCC CAGTCCTTTC CAGATGTGGA GCCCCAGCTG 
CCCCAAGCTC CAACCCGTGG GGGTAGTAGC CGAGAGGCTG TGCAGAGGGG TCTGAATTCA 
TGGCAACAGC AGCAGCCCCA CCCACCTCCC CGCCAGCAGG AGAGATCCCC ACTGCAGAGT 
CTTGCCCGCA GCAAGCCTAG CCCCCAGCTG TCAGCAGAGA CCCCCGTAGC TGCTCTCCCA 
GAGTTCCCAA GGGCAGCGTC CCAGCAGGAG ATCGAGCAGT CCATCGAAAC CCTCAATATG 
TTGATGCTGG ACCTGGAGCC AGCCTCCGCA GCTGCCCCTC TTCATAAGTC CCAGAGTGTC 
CCTGGGGCCT GGCCAGGGGC TTCTCCACTC TCTTCCCAGC CCCTCTTGGG GTCTTCCCGC 
CAGTCCCACC CACTTACCCA GTCCCGATCT GGCTATATCC CCAGTGGGCA TTCTCTGGGA 
ACCCCGGAGC TGGTCTCTTC TGGCAGGCCT TATTCACCTT ACGATTATCA GCTGCATCCA 
GCTGGATCCA ACCAGAGTTT CCATCCAAAA AGTCCAGCCT CTTCCACCTT CCTTCCAAGC 
CCTCATAGCT CTGCGGGGCC TCAGGAGCCC CCAGCCTCTC TCCCTGGTCT CATCGCTCAG 
CCCCAGCTCC CACCAAAGGA AACTACATCA GATCCCTCCC GAACTCCAGA GGAAGAGCCG 
CTGAACCTGG AAGGGCTGGT AGCCCATCGG GTAGCAGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGAGCCCCG GAGCTATGTG 
GAGTCCGTGG CGAGGACAGC GGTGGCAGGG CCTCGAGCTC AGGACGTTGA GCCTAAGAGC 
TTCAGCGCAC CAGCTGCTCA CGCCTATGGG CACGAGACAC CCCTGAGGAA CGGGACCCCA 
GGGGGCTCCT TTGTCTCCCC GAGCCCCCTC TCCACAAGCA GCCCCATCCT CAGTGCTGAC 
AGCACTTCCG TGGGCAGTTT CCCATCAGTG GTGAGCAGTG ACCAGGGTCC CCGGACACCC 
TTCCAGCCTA TGCTGGACTC CAGCATCCGC TCAGGCAGCT TGGGGCAGCC GAGCCCTGCA 
GCACTGAGTT ATCAGAGCTC CTCGCCTGTC CCTGTTGGTG GCAGCAGCTA CAACAGCCCT 
GACTACTCCC TCCAACCATT CAGCTCTTCT CCAGAAAGCC AAGGCCAGCC ACAGTACAGT 
GCGGCCAGTG TCCACATGGT ACCTGGGAGC CCTCAGGCAC GACACAGGAC AGTGGGTACC 
AACACTCCAC CTAGCCCTGG TTTTGGCCGG CGGGCCGTCA ACCCCACCAT GGCTGCCCCT 
GGTAGTCCCA GTTTGAGCCA TCGGCAGGTG ATGGGTCCAT CTGGTCCAGG TTTCCATGGT 
AACGTGGTTT CTGGCCACCC AGCCAGTGCA GCTACCACTC CTGGAAGCCC CAGCCTGGGC 
CGGCACCCAG TGGGAAGCCA TCAAGTTCCA GGCCTCCACA GCAGTGTGGT CACCACTCCG 
GGAAGCCCAA GCCTGGGCCG ACACCCTGGT GCCCACCAAG GGAATCTGGC CTCCAGTCTC 
CACAGCAATG CAGTCATCAG CCCTGGGAGC CCAAGCTTGG GCCGGCACCT GGGTGGGTCT 
GGATCCGTGG TTCCTGGAAG CCCCAGCTTG GACCGGCATG CAGCTTATGG TGGCTACTCT 
ACCCCTGAGG ACCGAAGACC AACACTGTCC CGGCAAAGCA GTGCCTCTGG CTACCAGGCC 
CCATCCACAC CGTCATTCCC TGTCTCTCCT GCCTACTACC CTGGCCTGAG CAGCCCTGCC 
ACCTCACCCT CGCCAGACTC AGCAGCCTTC CGGCAGGGGA GCCCCACACC AGCCTTACCA 
GAGAAGCGAA GAATGTCAGT GGGAGACCGG GCAGGCAGCC TCCCCAACTA TGCCACCATC 
AATGGGAAAG TGTCTTCCTC GCCTGTCGCC AATGGCATGG CCAGTGGGAG CAGCACCGTC 
TCCTTCTCTC ACACGCTTCC GGACTTTTCT AAGTATTCGA TGCCAGACAA TAGTCCGGAG 
ACTCGGGCTA AAGTGAAGTT TGTCCAGGAC ACTTCCAAGT ATTGGTACAA GCCTGAGATC 
TCCAGAGAAC AGGCCATCGC ACTCCTAAAG GACCAGGAGC CAGGAGCCTT CATCATCCGG 
GACAGCCACT CCTTCCGAGG GGCTTATGGG CTGGCCATGA AGGTGTCCTC ACCCCCTCCA 
ACCATCACTC AGCAGGGCAA GAAAGGAGAC ATGACCCATG AGCTGGTGAG GCACTTTCTG 
ATCGAGACAG GCCCTAGAGG AGTGAAGCTC AAGGGTTGTC CCAATGAGCC AAACTTTGGT 
TCCCTTTCTG CCCTGGTCTA TCAGCACTCC GTCATCCCAC TGGCCCTGCC CTGTAAACTG 
GTCATTCCAA GCCGAGACCC CACAGATGAA TCAAAAGATA GCTCAGGCCC TGCCAACTCA 
ACCACTGACC TGCTGAAGCA AGGAGCAGCT TGCAATGTGC TCTTCGTCAA CTCTGTGGAT 
ATGGAGTCAC TCACCGGGCC ACAGGCCATT TCCAAAGCCA CATCTGAGAC TCTGGCTGCC 
GACCCCACAC CGGCTGCCAC CATTGTTCAC TTCAAGGTCT CTGCCCAAGG AATCACACTG 
ACTGACAATC AAAGAAAGCT CTTCTTTAGA AGACACTACC CCCTCAACAC TGTCACCTTC 
TGTGACCTGG ATCCACAAGA AAGAAAGTGG ATGAAGACAG AGGGAGGCGC CCCTGCTAAG 
CTCTTTGGCT TCGTGGCCCG GAAGCAGGGC AGCACCACAG ACAACGCTTG CCACCTCTTC 
GCAGAGCTTG ACCCCAACCA GCCTGCGTCT GCCATCGTCA ACTTCGTCTC CAAGGTCATG 
CTGAGTGCTG GCCAGAAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( NM_001289895.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276824.1 Mus musculus tensin 1 (Tns1), transcript variant 2, mRNA.

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CloneID	OMu58480
Clone ID Related Accession (Same CDS sequence)	NM_001289895.1 , NM_001289895.2
Accession Version	NM_001289895.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC117757.23 and AC116419.32. On Jun 18, 2019 this sequence version replaced NM_001289895.1. Transcript Variant: This variant (2) lacks two in-frame exons in the 3' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGCA CCGTGAGCCT GGTGTGCTGC GAGGCGCTCG AGCCTCTGCC CTCCTGCGGC 
CCGCAGCCCC CCGGGACTCC GCCGGGCCCC GCGCGGCCCG AGCGCTGTGA GCCCGGGGGT 
GCGGCGCCCG ACCCGAGGAG GCGGCTGCTG CTTCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGAAG TGGTGGGACA CACCCAAGGG CCGCTGGATG GGAGCCTGTA CGCCAAGGTG 
AAGAAGAAGG ATTCCCTGAA TGGCAGTTCG GGGCCTGTCA CTACTGCACG GCCTGCCCTG 
TCAGCCACTC CCAACCACGT GGAACACACC CTGTCTGTGA GCAGCGACTC TGGCAACTCC 
ACAGCCTCCA CTAAGACAGA CAAGACCGAT GAGCCAGTCT CTGGGGCTAC CACTGCCCCT 
GCTGCCCTGA GTCCTCAGGA GAAGAAAGAG CTGGACCGCC TACTAAGTGG CTTTGGTGTG 
GATAGAGAAA AGCAAGGCGC CATGTACCGG GCTCAGCAAC TCAGATCCCA TCCAGGCGGG 
GGCCCGACTG TGCCCTCCCC TGGCCGCCAC ATTGTCCCAG CCCAGGTTCA CGTCAATGGT 
GGGGCCCTGG CATCTGAGCG GGAGACAGAC ATTCTAGATG ATGAGCTGCC CATCCAGGAC 
GGGCAGAGTG GGGGCAGCAT GGGAACGCTG TCCTCCCTGG ATGGGGTCAC CAACACCAGT 
GAAAGTGGCT ACCCAGAGAC CCTGTCCCCA CTGACCAATG GCCTGGACAA ACCCTATTCC 
ACAGAACCTG TGCTCAACGG CGGAGGCTAC CCCTATGAGG CTGCCAACCG GGTGATTCCT 
GTCCACAGCA GTCACTCGGC CCCCATTCGA CCCTCCTACT CTGCTCAGGA GGGTTTAGCT 
GGCTACCAGC GTGAGGGACC CCACCCAGCC TGGTCACAGC AAGTGACCAG TGCCCACTGT 
GGCTGTGACC CCAGTGGTCT ATTCAGATCC CAGTCCTTTC CAGATGTGGA GCCCCAGCTG 
CCCCAAGCTC CAACCCGTGG GGGTAGTAGC CGAGAGGCTG TGCAGAGGGG TCTGAATTCA 
TGGCAACAGC AGCAGCCCCA CCCACCTCCC CGCCAGCAGG AGAGATCCCC ACTGCAGAGT 
CTTGCCCGCA GCAAGCCTAG CCCCCAGCTG TCAGCAGAGA CCCCCGTAGC TGCTCTCCCA 
GAGTTCCCAA GGGCAGCGTC CCAGCAGGAG ATCGAGCAGT CCATCGAAAC CCTCAATATG 
TTGATGCTGG ACCTGGAGCC AGCCTCCGCA GCTGCCCCTC TTCATAAGTC CCAGAGTGTC 
CCTGGGGCCT GGCCAGGGGC TTCTCCACTC TCTTCCCAGC CCCTCTTGGG GTCTTCCCGC 
CAGTCCCACC CACTTACCCA GTCCCGATCT GGCTATATCC CCAGTGGGCA TTCTCTGGGA 
ACCCCGGAGC TGGTCTCTTC TGGCAGGCCT TATTCACCTT ACGATTATCA GCTGCATCCA 
GCTGGATCCA ACCAGAGTTT CCATCCAAAA AGTCCAGCCT CTTCCACCTT CCTTCCAAGC 
CCTCATAGCT CTGCGGGGCC TCAGGAGCCC CCAGCCTCTC TCCCTGGTCT CATCGCTCAG 
CCCCAGCTCC CACCAAAGGA AACTACATCA GATCCCTCCC GAACTCCAGA GGAAGAGCCG 
CTGAACCTGG AAGGGCTGGT AGCCCATCGG GTAGCAGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGAGCCCCG GAGCTATGTG 
GAGTCCGTGG CGAGGACAGC GGTGGCAGGG CCTCGAGCTC AGGACGTTGA GCCTAAGAGC 
TTCAGCGCAC CAGCTGCTCA CGCCTATGGG CACGAGACAC CCCTGAGGAA CGGGACCCCA 
GGGGGCTCCT TTGTCTCCCC GAGCCCCCTC TCCACAAGCA GCCCCATCCT CAGTGCTGAC 
AGCACTTCCG TGGGCAGTTT CCCATCAGTG GTGAGCAGTG ACCAGGGTCC CCGGACACCC 
TTCCAGCCTA TGCTGGACTC CAGCATCCGC TCAGGCAGCT TGGGGCAGCC GAGCCCTGCA 
GCACTGAGTT ATCAGAGCTC CTCGCCTGTC CCTGTTGGTG GCAGCAGCTA CAACAGCCCT 
GACTACTCCC TCCAACCATT CAGCTCTTCT CCAGAAAGCC AAGGCCAGCC ACAGTACAGT 
GCGGCCAGTG TCCACATGGT ACCTGGGAGC CCTCAGGCAC GACACAGGAC AGTGGGTACC 
AACACTCCAC CTAGCCCTGG TTTTGGCCGG CGGGCCGTCA ACCCCACCAT GGCTGCCCCT 
GGTAGTCCCA GTTTGAGCCA TCGGCAGGTG ATGGGTCCAT CTGGTCCAGG TTTCCATGGT 
AACGTGGTTT CTGGCCACCC AGCCAGTGCA GCTACCACTC CTGGAAGCCC CAGCCTGGGC 
CGGCACCCAG TGGGAAGCCA TCAAGTTCCA GGCCTCCACA GCAGTGTGGT CACCACTCCG 
GGAAGCCCAA GCCTGGGCCG ACACCCTGGT GCCCACCAAG GGAATCTGGC CTCCAGTCTC 
CACAGCAATG CAGTCATCAG CCCTGGGAGC CCAAGCTTGG GCCGGCACCT GGGTGGGTCT 
GGATCCGTGG TTCCTGGAAG CCCCAGCTTG GACCGGCATG CAGCTTATGG TGGCTACTCT 
ACCCCTGAGG ACCGAAGACC AACACTGTCC CGGCAAAGCA GTGCCTCTGG CTACCAGGCC 
CCATCCACAC CGTCATTCCC TGTCTCTCCT GCCTACTACC CTGGCCTGAG CAGCCCTGCC 
ACCTCACCCT CGCCAGACTC AGCAGCCTTC CGGCAGGGGA GCCCCACACC AGCCTTACCA 
GAGAAGCGAA GAATGTCAGT GGGAGACCGG GCAGGCAGCC TCCCCAACTA TGCCACCATC 
AATGGGAAAG TGTCTTCCTC GCCTGTCGCC AATGGCATGG CCAGTGGGAG CAGCACCGTC 
TCCTTCTCTC ACACGCTTCC GGACTTTTCT AAGTATTCGA TGCCAGACAA TAGTCCGGAG 
ACTCGGGCTA AAGTGAAGTT TGTCCAGGAC ACTTCCAAGT ATTGGTACAA GCCTGAGATC 
TCCAGAGAAC AGGCCATCGC ACTCCTAAAG GACCAGGAGC CAGGAGCCTT CATCATCCGG 
GACAGCCACT CCTTCCGAGG GGCTTATGGG CTGGCCATGA AGGTGTCCTC ACCCCCTCCA 
ACCATCACTC AGCAGGGCAA GAAAGGAGAC ATGACCCATG AGCTGGTGAG GCACTTTCTG 
ATCGAGACAG GCCCTAGAGG AGTGAAGCTC AAGGGTTGTC CCAATGAGCC AAACTTTGGT 
TCCCTTTCTG CCCTGGTCTA TCAGCACTCC GTCATCCCAC TGGCCCTGCC CTGTAAACTG 
GTCATTCCAA GCCGAGACCC CACAGATGAA TCAAAAGATA GCTCAGGCCC TGCCAACTCA 
ACCACTGACC TGCTGAAGCA AGGAGCAGCT TGCAATGTGC TCTTCGTCAA CTCTGTGGAT 
ATGGAGTCAC TCACCGGGCC ACAGGCCATT TCCAAAGCCA CATCTGAGAC TCTGGCTGCC 
GACCCCACAC CGGCTGCCAC CATTGTTCAC TTCAAGGTCT CTGCCCAAGG AATCACACTG 
ACTGACAATC AAAGAAAGCT CTTCTTTAGA AGACACTACC CCCTCAACAC TGTCACCTTC 
TGTGACCTGG ATCCACAAGA AAGAAAGTGG ATGAAGACAG AGGGAGGCGC CCCTGCTAAG 
CTCTTTGGCT TCGTGGCCCG GAAGCAGGGC AGCACCACAG ACAACGCTTG CCACCTCTTC 
GCAGAGCTTG ACCCCAACCA GCCTGCGTCT GCCATCGTCA ACTTCGTCTC CAAGGTCATG 
CTGAGTGCTG GCCAGAAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276824.1
CDS	102..5705
Translation	MGCTVSLVCCEALEPLPSCGPQPPGTPPGPARPERCEPGGAAPDPRRRLLLQPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEEVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	NM_001289895.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289895.2

ORF Insert Sequence:

ATGGGCTGCA CCGTGAGCCT GGTGTGCTGC GAGGCGCTCG AGCCTCTGCC CTCCTGCGGC 
CCGCAGCCCC CCGGGACTCC GCCGGGCCCC GCGCGGCCCG AGCGCTGTGA GCCCGGGGGT 
GCGGCGCCCG ACCCGAGGAG GCGGCTGCTG CTTCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGAAG TGGTGGGACA CACCCAAGGG CCGCTGGATG GGAGCCTGTA CGCCAAGGTG 
AAGAAGAAGG ATTCCCTGAA TGGCAGTTCG GGGCCTGTCA CTACTGCACG GCCTGCCCTG 
TCAGCCACTC CCAACCACGT GGAACACACC CTGTCTGTGA GCAGCGACTC TGGCAACTCC 
ACAGCCTCCA CTAAGACAGA CAAGACCGAT GAGCCAGTCT CTGGGGCTAC CACTGCCCCT 
GCTGCCCTGA GTCCTCAGGA GAAGAAAGAG CTGGACCGCC TACTAAGTGG CTTTGGTGTG 
GATAGAGAAA AGCAAGGCGC CATGTACCGG GCTCAGCAAC TCAGATCCCA TCCAGGCGGG 
GGCCCGACTG TGCCCTCCCC TGGCCGCCAC ATTGTCCCAG CCCAGGTTCA CGTCAATGGT 
GGGGCCCTGG CATCTGAGCG GGAGACAGAC ATTCTAGATG ATGAGCTGCC CATCCAGGAC 
GGGCAGAGTG GGGGCAGCAT GGGAACGCTG TCCTCCCTGG ATGGGGTCAC CAACACCAGT 
GAAAGTGGCT ACCCAGAGAC CCTGTCCCCA CTGACCAATG GCCTGGACAA ACCCTATTCC 
ACAGAACCTG TGCTCAACGG CGGAGGCTAC CCCTATGAGG CTGCCAACCG GGTGATTCCT 
GTCCACAGCA GTCACTCGGC CCCCATTCGA CCCTCCTACT CTGCTCAGGA GGGTTTAGCT 
GGCTACCAGC GTGAGGGACC CCACCCAGCC TGGTCACAGC AAGTGACCAG TGCCCACTGT 
GGCTGTGACC CCAGTGGTCT ATTCAGATCC CAGTCCTTTC CAGATGTGGA GCCCCAGCTG 
CCCCAAGCTC CAACCCGTGG GGGTAGTAGC CGAGAGGCTG TGCAGAGGGG TCTGAATTCA 
TGGCAACAGC AGCAGCCCCA CCCACCTCCC CGCCAGCAGG AGAGATCCCC ACTGCAGAGT 
CTTGCCCGCA GCAAGCCTAG CCCCCAGCTG TCAGCAGAGA CCCCCGTAGC TGCTCTCCCA 
GAGTTCCCAA GGGCAGCGTC CCAGCAGGAG ATCGAGCAGT CCATCGAAAC CCTCAATATG 
TTGATGCTGG ACCTGGAGCC AGCCTCCGCA GCTGCCCCTC TTCATAAGTC CCAGAGTGTC 
CCTGGGGCCT GGCCAGGGGC TTCTCCACTC TCTTCCCAGC CCCTCTTGGG GTCTTCCCGC 
CAGTCCCACC CACTTACCCA GTCCCGATCT GGCTATATCC CCAGTGGGCA TTCTCTGGGA 
ACCCCGGAGC TGGTCTCTTC TGGCAGGCCT TATTCACCTT ACGATTATCA GCTGCATCCA 
GCTGGATCCA ACCAGAGTTT CCATCCAAAA AGTCCAGCCT CTTCCACCTT CCTTCCAAGC 
CCTCATAGCT CTGCGGGGCC TCAGGAGCCC CCAGCCTCTC TCCCTGGTCT CATCGCTCAG 
CCCCAGCTCC CACCAAAGGA AACTACATCA GATCCCTCCC GAACTCCAGA GGAAGAGCCG 
CTGAACCTGG AAGGGCTGGT AGCCCATCGG GTAGCAGGGG TTCAGGCTCG GGAGAGGCAG 
CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG CGGGCAGCCA GTGATGGACA GTATGAAAAC 
CAGTCTCCGG AGGCCACATC CCCCCGTAGT CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC 
TCCCCTGAGC TGGCTCTTAC CATTGCCCTC AATCCTGGAG GGCGCCCCAA AGAGCCTCAT 
TTGCATAGCT ACAAGGAGGC CTTTGAGGAG ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA 
CACAGTGTGG CGCGCTCTCC TCCAGGTCTG GCCAAGACAC CCCTGTCTGC TCTGGGCCTG 
AAACCCCACA ACCCAGCAGA CATCTTGCTG CACCCCACAG GTGAGCCCCG GAGCTATGTG 
GAGTCCGTGG CGAGGACAGC GGTGGCAGGG CCTCGAGCTC AGGACGTTGA GCCTAAGAGC 
TTCAGCGCAC CAGCTGCTCA CGCCTATGGG CACGAGACAC CCCTGAGGAA CGGGACCCCA 
GGGGGCTCCT TTGTCTCCCC GAGCCCCCTC TCCACAAGCA GCCCCATCCT CAGTGCTGAC 
AGCACTTCCG TGGGCAGTTT CCCATCAGTG GTGAGCAGTG ACCAGGGTCC CCGGACACCC 
TTCCAGCCTA TGCTGGACTC CAGCATCCGC TCAGGCAGCT TGGGGCAGCC GAGCCCTGCA 
GCACTGAGTT ATCAGAGCTC CTCGCCTGTC CCTGTTGGTG GCAGCAGCTA CAACAGCCCT 
GACTACTCCC TCCAACCATT CAGCTCTTCT CCAGAAAGCC AAGGCCAGCC ACAGTACAGT 
GCGGCCAGTG TCCACATGGT ACCTGGGAGC CCTCAGGCAC GACACAGGAC AGTGGGTACC 
AACACTCCAC CTAGCCCTGG TTTTGGCCGG CGGGCCGTCA ACCCCACCAT GGCTGCCCCT 
GGTAGTCCCA GTTTGAGCCA TCGGCAGGTG ATGGGTCCAT CTGGTCCAGG TTTCCATGGT 
AACGTGGTTT CTGGCCACCC AGCCAGTGCA GCTACCACTC CTGGAAGCCC CAGCCTGGGC 
CGGCACCCAG TGGGAAGCCA TCAAGTTCCA GGCCTCCACA GCAGTGTGGT CACCACTCCG 
GGAAGCCCAA GCCTGGGCCG ACACCCTGGT GCCCACCAAG GGAATCTGGC CTCCAGTCTC 
CACAGCAATG CAGTCATCAG CCCTGGGAGC CCAAGCTTGG GCCGGCACCT GGGTGGGTCT 
GGATCCGTGG TTCCTGGAAG CCCCAGCTTG GACCGGCATG CAGCTTATGG TGGCTACTCT 
ACCCCTGAGG ACCGAAGACC AACACTGTCC CGGCAAAGCA GTGCCTCTGG CTACCAGGCC 
CCATCCACAC CGTCATTCCC TGTCTCTCCT GCCTACTACC CTGGCCTGAG CAGCCCTGCC 
ACCTCACCCT CGCCAGACTC AGCAGCCTTC CGGCAGGGGA GCCCCACACC AGCCTTACCA 
GAGAAGCGAA GAATGTCAGT GGGAGACCGG GCAGGCAGCC TCCCCAACTA TGCCACCATC 
AATGGGAAAG TGTCTTCCTC GCCTGTCGCC AATGGCATGG CCAGTGGGAG CAGCACCGTC 
TCCTTCTCTC ACACGCTTCC GGACTTTTCT AAGTATTCGA TGCCAGACAA TAGTCCGGAG 
ACTCGGGCTA AAGTGAAGTT TGTCCAGGAC ACTTCCAAGT ATTGGTACAA GCCTGAGATC 
TCCAGAGAAC AGGCCATCGC ACTCCTAAAG GACCAGGAGC CAGGAGCCTT CATCATCCGG 
GACAGCCACT CCTTCCGAGG GGCTTATGGG CTGGCCATGA AGGTGTCCTC ACCCCCTCCA 
ACCATCACTC AGCAGGGCAA GAAAGGAGAC ATGACCCATG AGCTGGTGAG GCACTTTCTG 
ATCGAGACAG GCCCTAGAGG AGTGAAGCTC AAGGGTTGTC CCAATGAGCC AAACTTTGGT 
TCCCTTTCTG CCCTGGTCTA TCAGCACTCC GTCATCCCAC TGGCCCTGCC CTGTAAACTG 
GTCATTCCAA GCCGAGACCC CACAGATGAA TCAAAAGATA GCTCAGGCCC TGCCAACTCA 
ACCACTGACC TGCTGAAGCA AGGAGCAGCT TGCAATGTGC TCTTCGTCAA CTCTGTGGAT 
ATGGAGTCAC TCACCGGGCC ACAGGCCATT TCCAAAGCCA CATCTGAGAC TCTGGCTGCC 
GACCCCACAC CGGCTGCCAC CATTGTTCAC TTCAAGGTCT CTGCCCAAGG AATCACACTG 
ACTGACAATC AAAGAAAGCT CTTCTTTAGA AGACACTACC CCCTCAACAC TGTCACCTTC 
TGTGACCTGG ATCCACAAGA AAGAAAGTGG ATGAAGACAG AGGGAGGCGC CCCTGCTAAG 
CTCTTTGGCT TCGTGGCCCG GAAGCAGGGC AGCACCACAG ACAACGCTTG CCACCTCTTC 
GCAGAGCTTG ACCCCAACCA GCCTGCGTCT GCCATCGTCA ACTTCGTCTC CAAGGTCATG 
CTGAGTGCTG GCCAGAAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tns1 ( XM_006495871.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006495934.1 Mus musculus tensin 1 (Tns1), transcript variant X6, mRNA.

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CloneID	OMu27359
Clone ID Related Accession (Same CDS sequence)	XM_006495871.3 , XM_006495871.4
Accession Version	XM_006495871.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tensin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006495871.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCA CCGTGAGCCT GGTGTGCTGC GAGGCGCTCG AGCCTCTGCC CTCCTGCGGC 
CCGCAGCCCC CCGGGACTCC GCCGGGCCCC GCGCGGCCCG AGCGCTGTGA GCCCGGGGGT 
GCGGCGCCCG ACCCGAGGAG GCGGCTGCTG CTTCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGGTT TAAAAGTGGT GGGACACACC CAAGGGCCGC TGGATGGGAG CCTGTACGCC 
AAGGTGAAGA AGAAGGATTC CCTGAATGGC AGTTCGGGGC CTGTCACTAC TGCACGGCCT 
GCCCTGTCAG CCACTCCCAA CCACGTGGAA CACACCCTGT CTGTGAGCAG CGACTCTGGC 
AACTCCACAG CCTCCACTAA GACAGACAAG ACCGATGAGC CAGTCTCTGG GGCTACCACT 
GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG AAAGAGCTGG ACCGCCTACT AAGTGGCTTT 
GGTGTGGATA GAGAAAAGCA AGGCGCCATG TACCGGGCTC AGCAACTCAG ATCCCATCCA 
GGCGGGGGCC CGACTGTGCC CTCCCCTGGC CGCCACATTG TCCCAGCCCA GGTTCACGTC 
AATGGTGGGG CCCTGGCATC TGAGCGGGAG ACAGACATTC TAGATGATGA GCTGCCCATC 
CAGGACGGGC AGAGTGGGGG CAGCATGGGA ACGCTGTCCT CCCTGGATGG GGTCACCAAC 
ACCAGTGAAA GTGGCTACCC AGAGACCCTG TCCCCACTGA CCAATGGCCT GGACAAACCC 
TATTCCACAG AACCTGTGCT CAACGGCGGA GGCTACCCCT ATGAGGCTGC CAACCGGGTG 
ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC ATTCGACCCT CCTACTCTGC TCAGGAGGGT 
TTAGCTGGCT ACCAGCGTGA GGGACCCCAC CCAGCCTGGT CACAGCAAGT GACCAGTGCC 
CACTGTGGCT GTGACCCCAG TGGTCTATTC AGATCCCAGT CCTTTCCAGA TGTGGAGCCC 
CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG 
AATTCATGGC AACAGCAGCA GCCCCACCCA CCTCCCCGCC AGCAGGAGAG ATCCCCACTG 
CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC CAGCTGTCAG CAGAGACCCC CGTAGCTGCT 
CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG CAGGAGATCG AGCAGTCCAT CGAAACCCTC 
AATATGTTGA TGCTGGACCT GGAGCCAGCC TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG 
AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT CCACTCTCTT CCCAGCCCCT CTTGGGGTCT 
TCCCGCCAGT CCCACCCACT TACCCAGTCC CGATCTGGCT ATATCCCCAG TGGGCATTCT 
CTGGGAACCC CGGAGCTGGT CTCTTCTGGC AGGCCTTATT CACCTTACGA TTATCAGCTG 
CATCCAGCTG GATCCAACCA GAGTTTCCAT CCAAAAAGTC CAGCCTCTTC CACCTTCCTT 
CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG GAGCCCCCAG CCTCTCTCCC TGGTCTCATC 
GCTCAGCCCC AGCTCCCACC AAAGGAAACT ACATCAGATC CCTCCCGAAC TCCAGAGGAA 
GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC CATCGGGTAG CAGTGTACAA TGCCAGACTA 
CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC 
CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG 
CGGGCAGCCA GTGATGGACA GTATGAAAAC CAGTCTCCGG AGGCCACATC CCCCCGTAGT 
CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC TCCCCTGAGC TGGCTCTTAC CATTGCCCTC 
AATCCTGGAG GGCGCCCCAA AGAGCCTCAT TTGCATAGCT ACAAGGAGGC CTTTGAGGAG 
ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA CACAGTGTGG CGCGCTCTCC TCCAGGTCTG 
GCCAAGACAC CCCTGTCTGC TCTGGGCCTG AAACCCCACA ACCCAGCAGA CATCTTGCTG 
CACCCCACAG GTGTTGCCAG AAGACTGATC CAGCCAGAGG AAGACGAGGG GGAGGAGGTG 
ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA 
GGGCCTCGAG CTCAGGACGT TGAGCCTAAG AGCTTCAGCG CACCAGCTGC TCACGCCTAT 
GGGCACGAGA CACCCCTGAG GAACGGGACC CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC 
CTCTCCACAA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CCGTGGGCAG TTTCCCATCA 
GTGGTGAGCA GTGACCAGGG TCCCCGGACA CCCTTCCAGC CTATGCTGGA CTCCAGCATC 
CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT GCAGCACTGA GTTATCAGAG CTCCTCGCCT 
GTCCCTGTTG GTGGCAGCAG CTACAACAGC CCTGACTACT CCCTCCAACC ATTCAGCTCT 
TCTCCAGAAA GCCAAGGCCA GCCACAGTAC AGTGCGGCCA GTGTCCACAT GGTACCTGGG 
AGCCCTCAGG CACGACACAG GACAGTGGGT ACCAACACTC CACCTAGCCC TGGTTTTGGC 
CGGCGGGCCG TCAACCCCAC CATGGCTGCC CCTGGTAGTC CCAGTTTGAG CCATCGGCAG 
GTGATGGGTC CATCTGGTCC AGGTTTCCAT GGTAACGTGG TTTCTGGCCA CCCAGCCAGT 
GCAGCTACCA CTCCTGGAAG CCCCAGCCTG GGCCGGCACC CAGTGGGAAG CCATCAAGTT 
CCAGGCCTCC ACAGCAGTGT GGTCACCACT CCGGGAAGCC CAAGCCTGGG CCGACACCCT 
GGTGCCCACC AAGGGAATCT GGCCTCCAGT CTCCACAGCA ATGCAGTCAT CAGCCCTGGG 
AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG TCTGGATCCG TGGTTCCTGG AAGCCCCAGC 
TTGGACCGGC ATGCAGCTTA TGGTGGCTAC TCTACCCCTG AGGACCGAAG ACCAACACTG 
TCCCGGCAAA GCAGTGCCTC TGGCTACCAG GCCCCATCCA CACCGTCATT CCCTGTCTCT 
CCTGCCTACT ACCCTGGCCT GAGCAGCCCT GCCACCTCAC CCTCGCCAGA CTCAGCAGCC 
TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA CCAGAGAAGC GAAGAATGTC AGTGGGAGAC 
CGGGCAGGCA GCCTCCCCAA CTATGCCACC ATCAATGGGA AAGTGTCTTC CTCGCCTGTC 
GCCAATGGCA TGGCCAGTGG GAGCAGCACC GTCTCCTTCT CTCACACGCT TCCGGACTTT 
TCTAAGTATT CGATGCCAGA CAATAGTCCG GAGACTCGGG CTAAAGTGAA GTTTGTCCAG 
GACACTTCCA AGTATTGGTA CAAGCCTGAG ATCTCCAGAG AACAGGCCAT CGCACTCCTA 
AAGGACCAGG AGCCAGGAGC CTTCATCATC CGGGACAGCC ACTCCTTCCG AGGGGCTTAT 
GGGCTGGCCA TGAAGGTGTC CTCACCCCCT CCAACCATCA CTCAGCAGGG CAAGAAAGGA 
GACATGACCC ATGAGCTGGT GAGGCACTTT CTGATCGAGA CAGGCCCTAG AGGAGTGAAG 
CTCAAGGGTT GTCCCAATGA GCCAAACTTT GGTTCCCTTT CTGCCCTGGT CTATCAGCAC 
TCCGTCATCC CACTGGCCCT GCCCTGTAAA CTGGTCATTC CAAGCCGAGA CCCCACAGAT 
GAATCAAAAG ATAGCTCAGG CCCTGCCAAC TCAACCACTG ACCTGCTGAA GCAAGGAGCA 
GCTTGCAATG TGCTCTTCGT CAACTCTGTG GATATGGAGT CACTCACCGG GCCACAGGCC 
ATTTCCAAAG CCACATCTGA GACTCTGGCT GCCGACCCCA CACCGGCTGC CACCATTGTT 
CACTTCAAGG TCTCTGCCCA AGGAATCACA CTGACTGACA ATCAAAGAAA GCTCTTCTTT 
AGAAGACACT ACCCCCTCAA CACTGTCACC TTCTGTGACC TGGATCCACA AGAAAGAAAG 
TGGATGAAGA CAGAGGGAGG CGCCCCTGCT AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG 
GGCAGCACCA CAGACAACGC TTGCCACCTC TTCGCAGAGC TTGACCCCAA CCAGCCTGCG 
TCTGCCATCG TCAACTTCGT CTCCAAGGTC ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006495934.1
CDS	83..5839
Translation	MGCTVSLVCCEALEPLPSCGPQPPGTPPGPARPERCEPGGAAPDPRRRLLLQPEDLEAPKTHHFKVKAFKKVKPCGICRQAITREGCVCKVCSFSCHRKCQAKVAAPCVPPSSHELVPITTETVPKNVVDVGEGDCRVGSSPKNLEEGGSMRVSPSIQPQPQSQPTSLSRNTSVSRAMEDSCELDLVYVTERIIAVSFPSTANEENFRSNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHNVVVLHNKGNRGRIGVVIAAYLHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSIKMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDILLKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFSGHREDGMEGLKVVGHTQGPLDGSLYAKVKKKDSLNGSSGPVTTARPALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGATTAPAALSPQEKKELDRLLSGFGVDREKQGAMYRAQQLRSHPGGGPTVPSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTNTSESGYPETLSPLTNGLDKPYSTEPVLNGGGYPYEAANRVIPVHSSHSAPIRPSYSAQEGLAGYQREGPHPAWSQQVTSAHCGCDPSGLFRSQSFPDVEPQLPQAPTRGGSSREAVQRGLNSWQQQQPHPPPRQQERSPLQSLARSKPSPQLSAETPVAALPEFPRAASQQEIEQSIETLNMLMLDLEPASAAAPLHKSQSVPGAWPGASPLSSQPLLGSSRQSHPLTQSRSGYIPSGHSLGTPELVSSGRPYSPYDYQLHPAGSNQSFHPKSPASSTFLPSPHSSAGPQEPPASLPGLIAQPQLPPKETTSDPSRTPEEEPLNLEGLVAHRVAVYNARLQSLRQSRGFQPSPHWRRSASLSGVQARERQPAEPPGPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHLHSYKEAFEEMEGTSPSSPPHSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVTKPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPAAHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSADSTSVGSFPSVVSSDQGPRTPFQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYNSPDYSLQPFSSSPESQGQPQYSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAVNPTMAAPGSPSLSHRQVMGPSGPGFHGNVVSGHPASAATTPGSPSLGRHPVGSHQVPGLHSSVVTTPGSPSLGRHPGAHQGNLASSLHSNAVISPGSPSLGRHLGGSGSVVPGSPSLDRHAAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPATSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASGSSTVSFSHTLPDFSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSPPPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPTDESKDSSGPANSTTDLLKQGAACNVLFVNSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGITLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQPASAIVNFVSKVMLSAGQKR

Target ORF information:

RefSeq Version	XM_006495871.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tensin 1 (Tns1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006495871.3

ORF Insert Sequence:

ATGGGCTGCA CCGTGAGCCT GGTGTGCTGC GAGGCGCTCG AGCCTCTGCC CTCCTGCGGC 
CCGCAGCCCC CCGGGACTCC GCCGGGCCCC GCGCGGCCCG AGCGCTGTGA GCCCGGGGGT 
GCGGCGCCCG ACCCGAGGAG GCGGCTGCTG CTTCAGCCAG AAGACCTGGA GGCCCCCAAG 
ACCCACCACT TCAAGGTGAA GGCCTTCAAG AAGGTGAAGC CCTGTGGCAT CTGCCGGCAG 
GCCATCACTC GGGAAGGCTG TGTTTGCAAA GTCTGCAGCT TCTCCTGTCA TCGGAAATGC 
CAGGCCAAGG TGGCTGCCCC TTGCGTTCCT CCGTCCAGCC ATGAACTGGT GCCCATCACC 
ACAGAGACCG TACCCAAGAA TGTAGTAGAT GTGGGGGAAG GAGACTGCCG GGTTGGAAGT 
TCTCCGAAAA ACCTGGAGGA GGGCGGCTCC ATGAGAGTTT CTCCAAGCAT ACAGCCACAA 
CCACAATCAC AGCCTACCAG TCTCTCTAGA AACACGAGTG TGAGCCGAGC CATGGAAGAC 
AGTTGTGAGC TGGACCTGGT GTATGTCACC GAACGCATCA TCGCCGTGTC CTTCCCTAGC 
ACAGCCAATG AGGAGAACTT CCGCAGCAAC CTCCGTGAAG TGGCTCAGAT GTTGAAGTCC 
AAGCATGGGG GCAACTACCT GCTATTCAAC CTCTCTGAAC AGAGGCCTGA CATCACCAAG 
CTGCATGCTA AGGTCCTGGA ATTCGGCTGG CCTGACCTGC ACACACCGGC CCTGGAGAAG 
ATATGCAGTG TCTGTAAGGC CATGGACACC TGGCTCAATG CAGACCCCCA CAATGTCGTT 
GTTCTCCACA ACAAGGGAAA CCGAGGCAGG ATTGGGGTTG TCATTGCAGC GTATCTGCAC 
TACAGCAACA TTTCAGCCAG TGCTGACCAG GCTCTGGACC GGTTTGCAAT GAAGAGATTC 
TATGAGGACA AGATCGTGCC CATTGGCCAG CCATCCCAGA GGAGATATGT GCACTACTTC 
AGTGGCCTAC TCTCTGGCTC CATCAAAATG AACAACAAGC CTTTGTTTCT GCATCATGTG 
ATCATGCACG GCATCCCCAA CTTTGAGTCT AAAGGAGGGT GCCGGCCCTT TCTCCGCATC 
TACCAAGCCA TGCAACCTGT GTACACATCT GGCATTTACA ACATCCCTGG AGACAGCCAG 
GCCAGCATCT GTATCACCAT TGAACCAGGG CTGCTCTTGA AGGGAGACAT CTTGCTGAAG 
TGTTATCATA AGAAATTCCG GAGCCCGGCT CGAGACGTCA TCTTCCGAGT GCAGTTCCAC 
ACCTGTGCCA TCCATGACTT GGGGGTTGTT TTTGGGAAGG AAGATCTGGA TGAGGCCTTC 
AAAGATGATC GATTTCCTGA CTATGGCAAA GTGGAGTTTG TCTTTTCCTA TGGACCAGAG 
AAAATTCAAG GCATGGAACA CCTGGAGAAT GGGCCCAGCG TATCTGTAGA CTATAACACT 
TCTGACCCCC TCATCCGCTG GGACTCCTAT GACAACTTCA GTGGGCATCG TGAGGATGGC 
ATGGAAGGTT TAAAAGTGGT GGGACACACC CAAGGGCCGC TGGATGGGAG CCTGTACGCC 
AAGGTGAAGA AGAAGGATTC CCTGAATGGC AGTTCGGGGC CTGTCACTAC TGCACGGCCT 
GCCCTGTCAG CCACTCCCAA CCACGTGGAA CACACCCTGT CTGTGAGCAG CGACTCTGGC 
AACTCCACAG CCTCCACTAA GACAGACAAG ACCGATGAGC CAGTCTCTGG GGCTACCACT 
GCCCCTGCTG CCCTGAGTCC TCAGGAGAAG AAAGAGCTGG ACCGCCTACT AAGTGGCTTT 
GGTGTGGATA GAGAAAAGCA AGGCGCCATG TACCGGGCTC AGCAACTCAG ATCCCATCCA 
GGCGGGGGCC CGACTGTGCC CTCCCCTGGC CGCCACATTG TCCCAGCCCA GGTTCACGTC 
AATGGTGGGG CCCTGGCATC TGAGCGGGAG ACAGACATTC TAGATGATGA GCTGCCCATC 
CAGGACGGGC AGAGTGGGGG CAGCATGGGA ACGCTGTCCT CCCTGGATGG GGTCACCAAC 
ACCAGTGAAA GTGGCTACCC AGAGACCCTG TCCCCACTGA CCAATGGCCT GGACAAACCC 
TATTCCACAG AACCTGTGCT CAACGGCGGA GGCTACCCCT ATGAGGCTGC CAACCGGGTG 
ATTCCTGTCC ACAGCAGTCA CTCGGCCCCC ATTCGACCCT CCTACTCTGC TCAGGAGGGT 
TTAGCTGGCT ACCAGCGTGA GGGACCCCAC CCAGCCTGGT CACAGCAAGT GACCAGTGCC 
CACTGTGGCT GTGACCCCAG TGGTCTATTC AGATCCCAGT CCTTTCCAGA TGTGGAGCCC 
CAGCTGCCCC AAGCTCCAAC CCGTGGGGGT AGTAGCCGAG AGGCTGTGCA GAGGGGTCTG 
AATTCATGGC AACAGCAGCA GCCCCACCCA CCTCCCCGCC AGCAGGAGAG ATCCCCACTG 
CAGAGTCTTG CCCGCAGCAA GCCTAGCCCC CAGCTGTCAG CAGAGACCCC CGTAGCTGCT 
CTCCCAGAGT TCCCAAGGGC AGCGTCCCAG CAGGAGATCG AGCAGTCCAT CGAAACCCTC 
AATATGTTGA TGCTGGACCT GGAGCCAGCC TCCGCAGCTG CCCCTCTTCA TAAGTCCCAG 
AGTGTCCCTG GGGCCTGGCC AGGGGCTTCT CCACTCTCTT CCCAGCCCCT CTTGGGGTCT 
TCCCGCCAGT CCCACCCACT TACCCAGTCC CGATCTGGCT ATATCCCCAG TGGGCATTCT 
CTGGGAACCC CGGAGCTGGT CTCTTCTGGC AGGCCTTATT CACCTTACGA TTATCAGCTG 
CATCCAGCTG GATCCAACCA GAGTTTCCAT CCAAAAAGTC CAGCCTCTTC CACCTTCCTT 
CCAAGCCCTC ATAGCTCTGC GGGGCCTCAG GAGCCCCCAG CCTCTCTCCC TGGTCTCATC 
GCTCAGCCCC AGCTCCCACC AAAGGAAACT ACATCAGATC CCTCCCGAAC TCCAGAGGAA 
GAGCCGCTGA ACCTGGAAGG GCTGGTAGCC CATCGGGTAG CAGTGTACAA TGCCAGACTA 
CAGAGCCTGA GGCAGAGCCG CGGCTTCCAA CCCTCTCCCC ACTGGCGCCG ATCTGCCTCC 
CTCTCTGGGG TTCAGGCTCG GGAGAGGCAG CCTGCAGAGC CCCCAGGCCC ACTCCGGAGG 
CGGGCAGCCA GTGATGGACA GTATGAAAAC CAGTCTCCGG AGGCCACATC CCCCCGTAGT 
CCTGGGGTCC GCTCCCCCGT CCAGTGCGTC TCCCCTGAGC TGGCTCTTAC CATTGCCCTC 
AATCCTGGAG GGCGCCCCAA AGAGCCTCAT TTGCATAGCT ACAAGGAGGC CTTTGAGGAG 
ATGGAGGGGA CCTCCCCAAG CAGCCCGCCA CACAGTGTGG CGCGCTCTCC TCCAGGTCTG 
GCCAAGACAC CCCTGTCTGC TCTGGGCCTG AAACCCCACA ACCCAGCAGA CATCTTGCTG 
CACCCCACAG GTGTTGCCAG AAGACTGATC CAGCCAGAGG AAGACGAGGG GGAGGAGGTG 
ACTAAGCCTC CAGAAGAGCC CCGGAGCTAT GTGGAGTCCG TGGCGAGGAC AGCGGTGGCA 
GGGCCTCGAG CTCAGGACGT TGAGCCTAAG AGCTTCAGCG CACCAGCTGC TCACGCCTAT 
GGGCACGAGA CACCCCTGAG GAACGGGACC CCAGGGGGCT CCTTTGTCTC CCCGAGCCCC 
CTCTCCACAA GCAGCCCCAT CCTCAGTGCT GACAGCACTT CCGTGGGCAG TTTCCCATCA 
GTGGTGAGCA GTGACCAGGG TCCCCGGACA CCCTTCCAGC CTATGCTGGA CTCCAGCATC 
CGCTCAGGCA GCTTGGGGCA GCCGAGCCCT GCAGCACTGA GTTATCAGAG CTCCTCGCCT 
GTCCCTGTTG GTGGCAGCAG CTACAACAGC CCTGACTACT CCCTCCAACC ATTCAGCTCT 
TCTCCAGAAA GCCAAGGCCA GCCACAGTAC AGTGCGGCCA GTGTCCACAT GGTACCTGGG 
AGCCCTCAGG CACGACACAG GACAGTGGGT ACCAACACTC CACCTAGCCC TGGTTTTGGC 
CGGCGGGCCG TCAACCCCAC CATGGCTGCC CCTGGTAGTC CCAGTTTGAG CCATCGGCAG 
GTGATGGGTC CATCTGGTCC AGGTTTCCAT GGTAACGTGG TTTCTGGCCA CCCAGCCAGT 
GCAGCTACCA CTCCTGGAAG CCCCAGCCTG GGCCGGCACC CAGTGGGAAG CCATCAAGTT 
CCAGGCCTCC ACAGCAGTGT GGTCACCACT CCGGGAAGCC CAAGCCTGGG CCGACACCCT 
GGTGCCCACC AAGGGAATCT GGCCTCCAGT CTCCACAGCA ATGCAGTCAT CAGCCCTGGG 
AGCCCAAGCT TGGGCCGGCA CCTGGGTGGG TCTGGATCCG TGGTTCCTGG AAGCCCCAGC 
TTGGACCGGC ATGCAGCTTA TGGTGGCTAC TCTACCCCTG AGGACCGAAG ACCAACACTG 
TCCCGGCAAA GCAGTGCCTC TGGCTACCAG GCCCCATCCA CACCGTCATT CCCTGTCTCT 
CCTGCCTACT ACCCTGGCCT GAGCAGCCCT GCCACCTCAC CCTCGCCAGA CTCAGCAGCC 
TTCCGGCAGG GGAGCCCCAC ACCAGCCTTA CCAGAGAAGC GAAGAATGTC AGTGGGAGAC 
CGGGCAGGCA GCCTCCCCAA CTATGCCACC ATCAATGGGA AAGTGTCTTC CTCGCCTGTC 
GCCAATGGCA TGGCCAGTGG GAGCAGCACC GTCTCCTTCT CTCACACGCT TCCGGACTTT 
TCTAAGTATT CGATGCCAGA CAATAGTCCG GAGACTCGGG CTAAAGTGAA GTTTGTCCAG 
GACACTTCCA AGTATTGGTA CAAGCCTGAG ATCTCCAGAG AACAGGCCAT CGCACTCCTA 
AAGGACCAGG AGCCAGGAGC CTTCATCATC CGGGACAGCC ACTCCTTCCG AGGGGCTTAT 
GGGCTGGCCA TGAAGGTGTC CTCACCCCCT CCAACCATCA CTCAGCAGGG CAAGAAAGGA 
GACATGACCC ATGAGCTGGT GAGGCACTTT CTGATCGAGA CAGGCCCTAG AGGAGTGAAG 
CTCAAGGGTT GTCCCAATGA GCCAAACTTT GGTTCCCTTT CTGCCCTGGT CTATCAGCAC 
TCCGTCATCC CACTGGCCCT GCCCTGTAAA CTGGTCATTC CAAGCCGAGA CCCCACAGAT 
GAATCAAAAG ATAGCTCAGG CCCTGCCAAC TCAACCACTG ACCTGCTGAA GCAAGGAGCA 
GCTTGCAATG TGCTCTTCGT CAACTCTGTG GATATGGAGT CACTCACCGG GCCACAGGCC 
ATTTCCAAAG CCACATCTGA GACTCTGGCT GCCGACCCCA CACCGGCTGC CACCATTGTT 
CACTTCAAGG TCTCTGCCCA AGGAATCACA CTGACTGACA ATCAAAGAAA GCTCTTCTTT 
AGAAGACACT ACCCCCTCAA CACTGTCACC TTCTGTGACC TGGATCCACA AGAAAGAAAG 
TGGATGAAGA CAGAGGGAGG CGCCCCTGCT AAGCTCTTTG GCTTCGTGGC CCGGAAGCAG 
GGCAGCACCA CAGACAACGC TTGCCACCTC TTCGCAGAGC TTGACCCCAA CCAGCCTGCG 
TCTGCCATCG TCAACTTCGT CTCCAAGGTC ATGCTGAGTG CTGGCCAGAA GAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tensin1 positively regulates RhoA activity through its interaction with DLC1.
Biochimica et biophysica acta1853(12)3258-65(2015 Dec)
Shih YP,Sun P,Wang A,Lo SH

Genetic association analyses highlight biological pathways underlying mitral valve prolapse.
Nature genetics47(10)1206-11(2015 Oct)
Dina C,Bouatia-Naji N,Tucker N,Delling FN,Toomer K,Durst R,Perrocheau M,Fernandez-Friera L,Solis J,,Le Tourneau T,Chen MH,Probst V,Bosse Y,Pibarot P,Zelenika D,Lathrop M,Hercberg S,Roussel R,Benjamin EJ,Bonnet F,Lo SH,Dolmatova E,Simonet F,Lecointe S,Kyndt F,Redon R,Le Marec H,Froguel P,Ellinor PT,Vasan RS,Bruneval P,Markwald RR,Norris RA,Milan DJ,Slaugenhaupt SA,Levine RA,Schott JJ,Hagege AA,,Jeunemaitre X,

Endothelial cell migration during murine yolk sac vascular remodeling occurs by means of a Rac1 and FAK activation pathway in vivo.
Developmental dynamics : an official publication of the American Association of Anatomists239(10)2570-83(2010 Oct)
Enciso JM,Konecny CM,Karpen HE,Hirschi KK

Rhombomere-specific analysis reveals the repertoire of genetic cues expressed across the developing hindbrain.
Neural development46(2009 Feb)
Chambers D,Wilson LJ,Alfonsi F,Hunter E,Saxena U,Blanc E,Lumsden A

Tagging genes with cassette-exchange sites.
Nucleic acids research33(4)e44(2005 Mar)
Cobellis G,Nicolaus G,Iovino M,Romito A,Marra E,Barbarisi M,Sardiello M,Di Giorgio FP,Iovino N,Zollo M,Ballabio A,Cortese R

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Tns1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: