ROBO4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ROBO4
Entrez Gene ID	54538
Full Name	roundabout guidance receptor 4
Synonyms	ECSM4,MRB
General protein information	Preferred Names roundabout guidance receptor 4 Names roundabout homolog 4 roundabout homolog 4, magic roundabout roundabout, axon guidance receptor, homolog 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q24.2
Disorder MIM:	607528

mRNA and Protein(s)

mRNA	Protein	Name
NM_019055.6	NP_061928.4	roundabout homolog 4 isoform 1 precursor
NM_001301088.1	NP_001288017.1	roundabout homolog 4 isoform 2
XM_006718861.1	XP_006718924.1	roundabout homolog 4 isoform X1
NM_001301088.2	NP_001288017.1	roundabout homolog 4 isoform 2
NM_019055.5	NP_061928.4	roundabout homolog 4 isoform 1 precursor
XM_011542875.1	XP_011541177.1	roundabout homolog 4 isoform X2
XM_006718861.2	XP_006718924.1	roundabout homolog 4 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ROBO4	XP_508841.3
Rattus norvegicus (Norway rat)	Robo4	NP_852040.1
Homo sapiens (human)	ROBO4	NP_061928.4
Canis lupus familiaris (dog)	ROBO4	XP_005619671.1
	ROBO4	XP_002799918.1
Bos taurus (cattle)	ROBO4	NP_001092661.1
Xenopus tropicalis (tropical clawed frog)	LOC100493397	XP_002937566.2
Mus musculus (house mouse)	Robo4	NP_083059.2
Rattus norvegicus (Norway rat)	LOC100911068	XP_006242881.1

Gene Ontology
Bibliography

Related articles in PubMed

Endothelial Robo4 regulates IL-6 production by endothelial cells and monocytes via a crosstalk mechanism in inflammation.
Shirakura K, Ishiba R, Kashio T, Sakai M, Fukushima Y, Yamamoto N, Manabe S, Shigesada N, Tanaka T, Hino N, Aird WC, Doi T, Okada Y
Biochemical and biophysical research communications495(1)801-806(2018 01)

Transcription factor SP1 mediates hyperglycemia-induced upregulation of roundabout4 in retinal microvascular endothelial cells.
Xie J, Gong Q, Liu X, Liu Z, Tian R, Cheng Y, Su G
Gene61631-40(2017 Jun)

Validation of RT-qPCR reference genes and determination of Robo4 expression levels in human retinal endothelial cells under hypoxia and/or hyperglycemia.
Xie J, Liu X, Li Y, Liu Y, Su G
Gene585(1)135-142(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GM-CSF and IL-1beta. Taken together, we demonstrated novel Robo4 functions in inflammation, i.e., it promotes IL-6 production by endothelial cells and immune cells via crosstalk.
Title: Endothelial Robo4 regulates IL-6 production by endothelial cells and monocytes via a crosstalk mechanism in inflammation.

Results show that hyperglycemia-induced upregulation of Robo4 in retinal endothelial cells is directly mediated by transcription factor SP1 which increases the transcription of Robo4 via an additional binding site in Robo4 promoter. SP1/Robo4 can regulate the migration, monolayer permeability and angiogenesis of retinal endothelial cells under hyperglycemic conditions.
Title: Transcription factor SP1 mediates hyperglycemia-induced upregulation of roundabout4 in retinal microvascular endothelial cells.

there was a increase in Robo4 expression under hyperglycemic conditions, while there was a decrease under hypoxic and combined hypoxic and hyperglycemic conditions, suggesting Robo4 might play different roles in various stages of diabetic retinopathy
Title: Validation of RT-qPCR reference genes and determination of Robo4 expression levels in human retinal endothelial cells under hypoxia and/or hyperglycemia.

Low expression level of ROBO4 is associated with evasive resistance and disease progression in cancer.
Title: Biomarkers of evasive resistance predict disease progression in cancer patients treated with antiangiogenic therapies.

These results suggest that Slit2/Robo1 binding exerts an effect on cell migration, which is negatively regulated by Robo4, and Robo1 may function by interacting with CdGAP in HUVECs.
Title: Suppression of Slit2/Robo1 mediated HUVEC migration by Robo4.

The following ROBO4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROBO4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu45805	NM_001301088.2 Latest version!	Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OHu68437	XM_011542875.1 Latest version!	Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu45804	XM_006718861.2 Latest version!	Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu09210	NM_019055.6 Latest version!	Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$384.30 $549.00
View Old Accession Versions >>
OHu45805	NM_001301088.1	Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OHu09210	NM_019055.5	Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$384.30 $549.00
OHu45804	XM_006718861.1	Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ROBO4 ( NM_001301088.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288017.1 Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu45805
Clone ID Related Accession (Same CDS sequence)	NM_001301088.1 , NM_001301088.2
Accession Version	NM_001301088.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-31
Organism	Homo sapiens(human)
Product	roundabout homolog 4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC424813.1, AK302628.1 and AF361473.2. On Jul 19, 2014 this sequence version replaced XM_005271596.2. Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of the second exon and starts translation at a downstream AUG compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.119610.1, AK302628.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGCTG TGGTGGGTGA GCAGTTTACT CTGGAATGTG GGCCGCCCTG GGGCCACCCA 
GAGCCCACAG TCTCATGGTG GAAAGATGGG AAACCCCTGG CCCTCCAGCC CGGAAGGCAC 
ACAGTGTCCG GGGGGTCCCT GCTGATGGCA AGAGCAGAGA AGAGTGACGA AGGGACCTAC 
ATGTGTGTGG CCACCAACAG CGCAGGACAT AGGGAGAGCC GCGCAGCCCG GGTTTCCATC 
CAGGAGCCCC AGGACTACAC GGAGCCTGTG GAGCTTCTGG CTGTGCGAAT TCAGCTGGAA 
AATGTGACAC TGCTGAACCC GGATCCTGCA GAGGGCCCCA AGCCTAGACC GGCGGTGTGG 
CTCAGCTGGA AGGTCAGTGG CCCTGCTGCG CCTGCCCAAT CTTACACGGC CTTGTTCAGG 
ACCCAGACTG CCCCGGGAGG CCAGGGAGCT CCGTGGGCAG AGGAGCTGCT GGCCGGCTGG 
CAGAGCGCAG AGCTTGGAGG CCTCCACTGG GGCCAAGACT ACGAGTTCAA AGTGAGACCA 
TCCTCTGGCC GGGCTCGAGG CCCTGACAGC AACGTGCTGC TCCTGAGGCT GCCGGAAAAA 
GTGCCCAGTG CCCCACCTCA GGAAGTGACT CTAAAGCCTG GCAATGGCAC TGTCTTTGTG 
AGCTGGGTCC CACCACCTGC TGAAAACCAC AATGGCATCA TCCGTGGCTA CCAGGTCTGG 
AGCCTGGGCA ACACATCACT GCCACCAGCC AACTGGACTG TAGTTGGTGA GCAGACCCAG 
CTGGAAATCG CCACCCATAT GCCAGGCTCC TACTGCGTGC AAGTGGCTGC AGTCACTGGT 
GCTGGAGCTG GGGAGCCCAG TAGACCTGTC TGCCTCCTTT TAGAGCAGGC CATGGAGCGA 
GCCACCCAAG AACCCAGTGA GCATGGTCCC TGGACCCTGG AGCAGCTGAG GGCTACCTTG 
AAGCGGCCTG AGGTCATTGC CACCTGCGGT GTTGCACTCT GGCTGCTGCT TCTGGGCACC 
GCCGTGTGTA TCCACCGCCG GCGCCGAGCT AGGGTGCACC TGGGCCCAGG TCTGTACAGA 
TATACCAGTG AGGATGCCAT CCTAAAACAC AGGATGGATC ACAGTGACTC CCAGTGGTTG 
GCAGACACTT GGCGTTCCAC CTCTGGCTCT CGGGACCTGA GCAGCAGCAG CAGCCTCAGC 
AGTCGGCTGG GGGCGGATGC CCGGGACCCA CTAGACTGTC GTCGCTCCTT GCTCTCCTGG 
GACTCCCGAA GCCCCGGCGT GCCCCTGCTT CCAGACACCA GCACTTTTTA TGGCTCCCTC 
ATCGCTGAGC TGCCCTCCAG TACCCCAGCC AGGCCAAGTC CCCAGGTCCC AGCTGTCAGG 
CGCCTCCCAC CCCAGCTGGC CCAGCTCTCC AGCCCCTGTT CCAGCTCAGA CAGCCTCTGC 
AGCCGCAGGG GACTCTCTTC TCCCCGCTTG TCTCTGGCCC CTGCAGAGGC TTGGAAGGCC 
AAAAAGAAGC AGGAGCTGCA GCATGCCAAC AGTTCCCCAC TGCTCCGGGG CAGCCACTCC 
TTGGAGCTCC GGGCCTGTGA GTTAGGAAAT AGAGGTTCCA AGAACCTTTC CCAAAGCCCA 
GGAGCTGTGC CCCAAGCTCT GGTTGCCTGG CGGGCCCTGG GACCGAAACT CCTCAGCTCC 
TCAAATGAGC TGGTTACTCG TCATCTCCCT CCAGCACCCC TCTTTCCTCA TGAAACTCCC 
CCAACTCAGA GTCAACAGAC CCAGCCTCCG GTGGCACCAC AGGCTCCCTC CTCCATCCTG 
CTGCCAGCAG CCCCCATCCC CATCCTTAGC CCCTGCAGTC CCCCTAGCCC CCAGGCCTCT 
TCCCTCTCTG GCCCCAGCCC AGCTTCCAGT CGCCTGTCCA GCTCCTCACT GTCATCCCTG 
GGGGAGGATC AAGACAGCGT GCTGACCCCT GAGGAGGTAG CCCTGTGCTT GGAACTCAGT 
GAGGGTGAGG AGACTCCCAG GAACAGCGTC TCTCCCATGC CAAGGGCTCC TTCACCCCCC 
ACCACCTATG GGTACATCAG CGTCCCAACA GCCTCAGAGT TCACGGACAT GGGCAGGACT 
GGAGGAGGGG TGGGGCCCAA GGGGGGAGTC TTGCTGTGCC CACCTCGGCC CTGCCTCACC 
CCCACCCCCA GCGAGGGCTC CTTAGCCAAT GGTTGGGGCT CAGCCTCTGA GGACAATGCC 
GCCAGCGCCA GAGCCAGCCT TGTCAGCTCC TCCGATGGCT CCTTCCTCGC TGATGCTCAC 
TTTGCCCGGG CCCTGGCAGT GGCTGTGGAT AGCTTTGGTT TCGGTCTAGA GCCCAGGGAG 
GCAGACTGCG TCTTCATAGA TGCCTCATCA CCTCCCTCCC CACGGGATGA GATCTTCCTG 
ACCCCCAACC TCTCCCTGCC CCTGTGGGAG TGGAGGCCAG ACTGGTTGGA AGACATGGAG 
GTCAGCCACA CCCAGCGGCT GGGAAGGGGG ATGCCTCCCT GGCCCCCTGA CTCTCAGATC 
TCTTCCCAGA GAAGTCAGCT CCACTGTCGT ATGCCCAAGG CTGGTGCTTC TCCTGTAGAT 
TACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288017.1
CDS	370..2958
Translation	MVAVVGEQFTLECGPPWGHPEPTVSWWKDGKPLALQPGRHTVSGGSLLMARAEKSDEGTYMCVATNSAGHRESRAARVSIQEPQDYTEPVELLAVRIQLENVTLLNPDPAEGPKPRPAVWLSWKVSGPAAPAQSYTALFRTQTAPGGQGAPWAEELLAGWQSAELGGLHWGQDYEFKVRPSSGRARGPDSNVLLLRLPEKVPSAPPQEVTLKPGNGTVFVSWVPPPAENHNGIIRGYQVWSLGNTSLPPANWTVVGEQTQLEIATHMPGSYCVQVAAVTGAGAGEPSRPVCLLLEQAMERATQEPSEHGPWTLEQLRATLKRPEVIATCGVALWLLLLGTAVCIHRRRRARVHLGPGLYRYTSEDAILKHRMDHSDSQWLADTWRSTSGSRDLSSSSSLSSRLGADARDPLDCRRSLLSWDSRSPGVPLLPDTSTFYGSLIAELPSSTPARPSPQVPAVRRLPPQLAQLSSPCSSSDSLCSRRGLSSPRLSLAPAEAWKAKKKQELQHANSSPLLRGSHSLELRACELGNRGSKNLSQSPGAVPQALVAWRALGPKLLSSSNELVTRHLPPAPLFPHETPPTQSQQTQPPVAPQAPSSILLPAAPIPILSPCSPPSPQASSLSGPSPASSRLSSSSLSSLGEDQDSVLTPEEVALCLELSEGEETPRNSVSPMPRAPSPPTTYGYISVPTASEFTDMGRTGGGVGPKGGVLLCPPRPCLTPTPSEGSLANGWGSASEDNAASARASLVSSSDGSFLADAHFARALAVAVDSFGFGLEPREADCVFIDASSPPSPRDEIFLTPNLSLPLWEWRPDWLEDMEVSHTQRLGRGMPPWPPDSQISSQRSQLHCRMPKAGASPVDYS

Target ORF information:

RefSeq Version	NM_001301088.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301088.1

ORF Insert Sequence:

ATGGTGGCTG TGGTGGGTGA GCAGTTTACT CTGGAATGTG GGCCGCCCTG GGGCCACCCA 
GAGCCCACAG TCTCATGGTG GAAAGATGGG AAACCCCTGG CCCTCCAGCC CGGAAGGCAC 
ACAGTGTCCG GGGGGTCCCT GCTGATGGCA AGAGCAGAGA AGAGTGACGA AGGGACCTAC 
ATGTGTGTGG CCACCAACAG CGCAGGACAT AGGGAGAGCC GCGCAGCCCG GGTTTCCATC 
CAGGAGCCCC AGGACTACAC GGAGCCTGTG GAGCTTCTGG CTGTGCGAAT TCAGCTGGAA 
AATGTGACAC TGCTGAACCC GGATCCTGCA GAGGGCCCCA AGCCTAGACC GGCGGTGTGG 
CTCAGCTGGA AGGTCAGTGG CCCTGCTGCG CCTGCCCAAT CTTACACGGC CTTGTTCAGG 
ACCCAGACTG CCCCGGGAGG CCAGGGAGCT CCGTGGGCAG AGGAGCTGCT GGCCGGCTGG 
CAGAGCGCAG AGCTTGGAGG CCTCCACTGG GGCCAAGACT ACGAGTTCAA AGTGAGACCA 
TCCTCTGGCC GGGCTCGAGG CCCTGACAGC AACGTGCTGC TCCTGAGGCT GCCGGAAAAA 
GTGCCCAGTG CCCCACCTCA GGAAGTGACT CTAAAGCCTG GCAATGGCAC TGTCTTTGTG 
AGCTGGGTCC CACCACCTGC TGAAAACCAC AATGGCATCA TCCGTGGCTA CCAGGTCTGG 
AGCCTGGGCA ACACATCACT GCCACCAGCC AACTGGACTG TAGTTGGTGA GCAGACCCAG 
CTGGAAATCG CCACCCATAT GCCAGGCTCC TACTGCGTGC AAGTGGCTGC AGTCACTGGT 
GCTGGAGCTG GGGAGCCCAG TAGACCTGTC TGCCTCCTTT TAGAGCAGGC CATGGAGCGA 
GCCACCCAAG AACCCAGTGA GCATGGTCCC TGGACCCTGG AGCAGCTGAG GGCTACCTTG 
AAGCGGCCTG AGGTCATTGC CACCTGCGGT GTTGCACTCT GGCTGCTGCT TCTGGGCACC 
GCCGTGTGTA TCCACCGCCG GCGCCGAGCT AGGGTGCACC TGGGCCCAGG TCTGTACAGA 
TATACCAGTG AGGATGCCAT CCTAAAACAC AGGATGGATC ACAGTGACTC CCAGTGGTTG 
GCAGACACTT GGCGTTCCAC CTCTGGCTCT CGGGACCTGA GCAGCAGCAG CAGCCTCAGC 
AGTCGGCTGG GGGCGGATGC CCGGGACCCA CTAGACTGTC GTCGCTCCTT GCTCTCCTGG 
GACTCCCGAA GCCCCGGCGT GCCCCTGCTT CCAGACACCA GCACTTTTTA TGGCTCCCTC 
ATCGCTGAGC TGCCCTCCAG TACCCCAGCC AGGCCAAGTC CCCAGGTCCC AGCTGTCAGG 
CGCCTCCCAC CCCAGCTGGC CCAGCTCTCC AGCCCCTGTT CCAGCTCAGA CAGCCTCTGC 
AGCCGCAGGG GACTCTCTTC TCCCCGCTTG TCTCTGGCCC CTGCAGAGGC TTGGAAGGCC 
AAAAAGAAGC AGGAGCTGCA GCATGCCAAC AGTTCCCCAC TGCTCCGGGG CAGCCACTCC 
TTGGAGCTCC GGGCCTGTGA GTTAGGAAAT AGAGGTTCCA AGAACCTTTC CCAAAGCCCA 
GGAGCTGTGC CCCAAGCTCT GGTTGCCTGG CGGGCCCTGG GACCGAAACT CCTCAGCTCC 
TCAAATGAGC TGGTTACTCG TCATCTCCCT CCAGCACCCC TCTTTCCTCA TGAAACTCCC 
CCAACTCAGA GTCAACAGAC CCAGCCTCCG GTGGCACCAC AGGCTCCCTC CTCCATCCTG 
CTGCCAGCAG CCCCCATCCC CATCCTTAGC CCCTGCAGTC CCCCTAGCCC CCAGGCCTCT 
TCCCTCTCTG GCCCCAGCCC AGCTTCCAGT CGCCTGTCCA GCTCCTCACT GTCATCCCTG 
GGGGAGGATC AAGACAGCGT GCTGACCCCT GAGGAGGTAG CCCTGTGCTT GGAACTCAGT 
GAGGGTGAGG AGACTCCCAG GAACAGCGTC TCTCCCATGC CAAGGGCTCC TTCACCCCCC 
ACCACCTATG GGTACATCAG CGTCCCAACA GCCTCAGAGT TCACGGACAT GGGCAGGACT 
GGAGGAGGGG TGGGGCCCAA GGGGGGAGTC TTGCTGTGCC CACCTCGGCC CTGCCTCACC 
CCCACCCCCA GCGAGGGCTC CTTAGCCAAT GGTTGGGGCT CAGCCTCTGA GGACAATGCC 
GCCAGCGCCA GAGCCAGCCT TGTCAGCTCC TCCGATGGCT CCTTCCTCGC TGATGCTCAC 
TTTGCCCGGG CCCTGGCAGT GGCTGTGGAT AGCTTTGGTT TCGGTCTAGA GCCCAGGGAG 
GCAGACTGCG TCTTCATAGA TGCCTCATCA CCTCCCTCCC CACGGGATGA GATCTTCCTG 
ACCCCCAACC TCTCCCTGCC CCTGTGGGAG TGGAGGCCAG ACTGGTTGGA AGACATGGAG 
GTCAGCCACA CCCAGCGGCT GGGAAGGGGG ATGCCTCCCT GGCCCCCTGA CTCTCAGATC 
TCTTCCCAGA GAAGTCAGCT CCACTGTCGT ATGCCCAAGG CTGGTGCTTC TCCTGTAGAT 
TACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO4 ( NM_001301088.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288017.1 Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu45805
Clone ID Related Accession (Same CDS sequence)	NM_001301088.1 , NM_001301088.2
Accession Version	NM_001301088.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	roundabout homolog 4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC424813.1, AK302628.1, AF361473.2 and AP003501.2. On May 31, 2019 this sequence version replaced NM_001301088.1. Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of the second exon and starts translation at a downstream AUG compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.119610.1, AK302628.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGCTG TGGTGGGTGA GCAGTTTACT CTGGAATGTG GGCCGCCCTG GGGCCACCCA 
GAGCCCACAG TCTCATGGTG GAAAGATGGG AAACCCCTGG CCCTCCAGCC CGGAAGGCAC 
ACAGTGTCCG GGGGGTCCCT GCTGATGGCA AGAGCAGAGA AGAGTGACGA AGGGACCTAC 
ATGTGTGTGG CCACCAACAG CGCAGGACAT AGGGAGAGCC GCGCAGCCCG GGTTTCCATC 
CAGGAGCCCC AGGACTACAC GGAGCCTGTG GAGCTTCTGG CTGTGCGAAT TCAGCTGGAA 
AATGTGACAC TGCTGAACCC GGATCCTGCA GAGGGCCCCA AGCCTAGACC GGCGGTGTGG 
CTCAGCTGGA AGGTCAGTGG CCCTGCTGCG CCTGCCCAAT CTTACACGGC CTTGTTCAGG 
ACCCAGACTG CCCCGGGAGG CCAGGGAGCT CCGTGGGCAG AGGAGCTGCT GGCCGGCTGG 
CAGAGCGCAG AGCTTGGAGG CCTCCACTGG GGCCAAGACT ACGAGTTCAA AGTGAGACCA 
TCCTCTGGCC GGGCTCGAGG CCCTGACAGC AACGTGCTGC TCCTGAGGCT GCCGGAAAAA 
GTGCCCAGTG CCCCACCTCA GGAAGTGACT CTAAAGCCTG GCAATGGCAC TGTCTTTGTG 
AGCTGGGTCC CACCACCTGC TGAAAACCAC AATGGCATCA TCCGTGGCTA CCAGGTCTGG 
AGCCTGGGCA ACACATCACT GCCACCAGCC AACTGGACTG TAGTTGGTGA GCAGACCCAG 
CTGGAAATCG CCACCCATAT GCCAGGCTCC TACTGCGTGC AAGTGGCTGC AGTCACTGGT 
GCTGGAGCTG GGGAGCCCAG TAGACCTGTC TGCCTCCTTT TAGAGCAGGC CATGGAGCGA 
GCCACCCAAG AACCCAGTGA GCATGGTCCC TGGACCCTGG AGCAGCTGAG GGCTACCTTG 
AAGCGGCCTG AGGTCATTGC CACCTGCGGT GTTGCACTCT GGCTGCTGCT TCTGGGCACC 
GCCGTGTGTA TCCACCGCCG GCGCCGAGCT AGGGTGCACC TGGGCCCAGG TCTGTACAGA 
TATACCAGTG AGGATGCCAT CCTAAAACAC AGGATGGATC ACAGTGACTC CCAGTGGTTG 
GCAGACACTT GGCGTTCCAC CTCTGGCTCT CGGGACCTGA GCAGCAGCAG CAGCCTCAGC 
AGTCGGCTGG GGGCGGATGC CCGGGACCCA CTAGACTGTC GTCGCTCCTT GCTCTCCTGG 
GACTCCCGAA GCCCCGGCGT GCCCCTGCTT CCAGACACCA GCACTTTTTA TGGCTCCCTC 
ATCGCTGAGC TGCCCTCCAG TACCCCAGCC AGGCCAAGTC CCCAGGTCCC AGCTGTCAGG 
CGCCTCCCAC CCCAGCTGGC CCAGCTCTCC AGCCCCTGTT CCAGCTCAGA CAGCCTCTGC 
AGCCGCAGGG GACTCTCTTC TCCCCGCTTG TCTCTGGCCC CTGCAGAGGC TTGGAAGGCC 
AAAAAGAAGC AGGAGCTGCA GCATGCCAAC AGTTCCCCAC TGCTCCGGGG CAGCCACTCC 
TTGGAGCTCC GGGCCTGTGA GTTAGGAAAT AGAGGTTCCA AGAACCTTTC CCAAAGCCCA 
GGAGCTGTGC CCCAAGCTCT GGTTGCCTGG CGGGCCCTGG GACCGAAACT CCTCAGCTCC 
TCAAATGAGC TGGTTACTCG TCATCTCCCT CCAGCACCCC TCTTTCCTCA TGAAACTCCC 
CCAACTCAGA GTCAACAGAC CCAGCCTCCG GTGGCACCAC AGGCTCCCTC CTCCATCCTG 
CTGCCAGCAG CCCCCATCCC CATCCTTAGC CCCTGCAGTC CCCCTAGCCC CCAGGCCTCT 
TCCCTCTCTG GCCCCAGCCC AGCTTCCAGT CGCCTGTCCA GCTCCTCACT GTCATCCCTG 
GGGGAGGATC AAGACAGCGT GCTGACCCCT GAGGAGGTAG CCCTGTGCTT GGAACTCAGT 
GAGGGTGAGG AGACTCCCAG GAACAGCGTC TCTCCCATGC CAAGGGCTCC TTCACCCCCC 
ACCACCTATG GGTACATCAG CGTCCCAACA GCCTCAGAGT TCACGGACAT GGGCAGGACT 
GGAGGAGGGG TGGGGCCCAA GGGGGGAGTC TTGCTGTGCC CACCTCGGCC CTGCCTCACC 
CCCACCCCCA GCGAGGGCTC CTTAGCCAAT GGTTGGGGCT CAGCCTCTGA GGACAATGCC 
GCCAGCGCCA GAGCCAGCCT TGTCAGCTCC TCCGATGGCT CCTTCCTCGC TGATGCTCAC 
TTTGCCCGGG CCCTGGCAGT GGCTGTGGAT AGCTTTGGTT TCGGTCTAGA GCCCAGGGAG 
GCAGACTGCG TCTTCATAGA TGCCTCATCA CCTCCCTCCC CACGGGATGA GATCTTCCTG 
ACCCCCAACC TCTCCCTGCC CCTGTGGGAG TGGAGGCCAG ACTGGTTGGA AGACATGGAG 
GTCAGCCACA CCCAGCGGCT GGGAAGGGGG ATGCCTCCCT GGCCCCCTGA CTCTCAGATC 
TCTTCCCAGA GAAGTCAGCT CCACTGTCGT ATGCCCAAGG CTGGTGCTTC TCCTGTAGAT 
TACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288017.1
CDS	300..2888
Translation	MVAVVGEQFTLECGPPWGHPEPTVSWWKDGKPLALQPGRHTVSGGSLLMARAEKSDEGTYMCVATNSAGHRESRAARVSIQEPQDYTEPVELLAVRIQLENVTLLNPDPAEGPKPRPAVWLSWKVSGPAAPAQSYTALFRTQTAPGGQGAPWAEELLAGWQSAELGGLHWGQDYEFKVRPSSGRARGPDSNVLLLRLPEKVPSAPPQEVTLKPGNGTVFVSWVPPPAENHNGIIRGYQVWSLGNTSLPPANWTVVGEQTQLEIATHMPGSYCVQVAAVTGAGAGEPSRPVCLLLEQAMERATQEPSEHGPWTLEQLRATLKRPEVIATCGVALWLLLLGTAVCIHRRRRARVHLGPGLYRYTSEDAILKHRMDHSDSQWLADTWRSTSGSRDLSSSSSLSSRLGADARDPLDCRRSLLSWDSRSPGVPLLPDTSTFYGSLIAELPSSTPARPSPQVPAVRRLPPQLAQLSSPCSSSDSLCSRRGLSSPRLSLAPAEAWKAKKKQELQHANSSPLLRGSHSLELRACELGNRGSKNLSQSPGAVPQALVAWRALGPKLLSSSNELVTRHLPPAPLFPHETPPTQSQQTQPPVAPQAPSSILLPAAPIPILSPCSPPSPQASSLSGPSPASSRLSSSSLSSLGEDQDSVLTPEEVALCLELSEGEETPRNSVSPMPRAPSPPTTYGYISVPTASEFTDMGRTGGGVGPKGGVLLCPPRPCLTPTPSEGSLANGWGSASEDNAASARASLVSSSDGSFLADAHFARALAVAVDSFGFGLEPREADCVFIDASSPPSPRDEIFLTPNLSLPLWEWRPDWLEDMEVSHTQRLGRGMPPWPPDSQISSQRSQLHCRMPKAGASPVDYS

Target ORF information:

RefSeq Version	NM_001301088.2
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301088.2

ORF Insert Sequence:

ATGGTGGCTG TGGTGGGTGA GCAGTTTACT CTGGAATGTG GGCCGCCCTG GGGCCACCCA 
GAGCCCACAG TCTCATGGTG GAAAGATGGG AAACCCCTGG CCCTCCAGCC CGGAAGGCAC 
ACAGTGTCCG GGGGGTCCCT GCTGATGGCA AGAGCAGAGA AGAGTGACGA AGGGACCTAC 
ATGTGTGTGG CCACCAACAG CGCAGGACAT AGGGAGAGCC GCGCAGCCCG GGTTTCCATC 
CAGGAGCCCC AGGACTACAC GGAGCCTGTG GAGCTTCTGG CTGTGCGAAT TCAGCTGGAA 
AATGTGACAC TGCTGAACCC GGATCCTGCA GAGGGCCCCA AGCCTAGACC GGCGGTGTGG 
CTCAGCTGGA AGGTCAGTGG CCCTGCTGCG CCTGCCCAAT CTTACACGGC CTTGTTCAGG 
ACCCAGACTG CCCCGGGAGG CCAGGGAGCT CCGTGGGCAG AGGAGCTGCT GGCCGGCTGG 
CAGAGCGCAG AGCTTGGAGG CCTCCACTGG GGCCAAGACT ACGAGTTCAA AGTGAGACCA 
TCCTCTGGCC GGGCTCGAGG CCCTGACAGC AACGTGCTGC TCCTGAGGCT GCCGGAAAAA 
GTGCCCAGTG CCCCACCTCA GGAAGTGACT CTAAAGCCTG GCAATGGCAC TGTCTTTGTG 
AGCTGGGTCC CACCACCTGC TGAAAACCAC AATGGCATCA TCCGTGGCTA CCAGGTCTGG 
AGCCTGGGCA ACACATCACT GCCACCAGCC AACTGGACTG TAGTTGGTGA GCAGACCCAG 
CTGGAAATCG CCACCCATAT GCCAGGCTCC TACTGCGTGC AAGTGGCTGC AGTCACTGGT 
GCTGGAGCTG GGGAGCCCAG TAGACCTGTC TGCCTCCTTT TAGAGCAGGC CATGGAGCGA 
GCCACCCAAG AACCCAGTGA GCATGGTCCC TGGACCCTGG AGCAGCTGAG GGCTACCTTG 
AAGCGGCCTG AGGTCATTGC CACCTGCGGT GTTGCACTCT GGCTGCTGCT TCTGGGCACC 
GCCGTGTGTA TCCACCGCCG GCGCCGAGCT AGGGTGCACC TGGGCCCAGG TCTGTACAGA 
TATACCAGTG AGGATGCCAT CCTAAAACAC AGGATGGATC ACAGTGACTC CCAGTGGTTG 
GCAGACACTT GGCGTTCCAC CTCTGGCTCT CGGGACCTGA GCAGCAGCAG CAGCCTCAGC 
AGTCGGCTGG GGGCGGATGC CCGGGACCCA CTAGACTGTC GTCGCTCCTT GCTCTCCTGG 
GACTCCCGAA GCCCCGGCGT GCCCCTGCTT CCAGACACCA GCACTTTTTA TGGCTCCCTC 
ATCGCTGAGC TGCCCTCCAG TACCCCAGCC AGGCCAAGTC CCCAGGTCCC AGCTGTCAGG 
CGCCTCCCAC CCCAGCTGGC CCAGCTCTCC AGCCCCTGTT CCAGCTCAGA CAGCCTCTGC 
AGCCGCAGGG GACTCTCTTC TCCCCGCTTG TCTCTGGCCC CTGCAGAGGC TTGGAAGGCC 
AAAAAGAAGC AGGAGCTGCA GCATGCCAAC AGTTCCCCAC TGCTCCGGGG CAGCCACTCC 
TTGGAGCTCC GGGCCTGTGA GTTAGGAAAT AGAGGTTCCA AGAACCTTTC CCAAAGCCCA 
GGAGCTGTGC CCCAAGCTCT GGTTGCCTGG CGGGCCCTGG GACCGAAACT CCTCAGCTCC 
TCAAATGAGC TGGTTACTCG TCATCTCCCT CCAGCACCCC TCTTTCCTCA TGAAACTCCC 
CCAACTCAGA GTCAACAGAC CCAGCCTCCG GTGGCACCAC AGGCTCCCTC CTCCATCCTG 
CTGCCAGCAG CCCCCATCCC CATCCTTAGC CCCTGCAGTC CCCCTAGCCC CCAGGCCTCT 
TCCCTCTCTG GCCCCAGCCC AGCTTCCAGT CGCCTGTCCA GCTCCTCACT GTCATCCCTG 
GGGGAGGATC AAGACAGCGT GCTGACCCCT GAGGAGGTAG CCCTGTGCTT GGAACTCAGT 
GAGGGTGAGG AGACTCCCAG GAACAGCGTC TCTCCCATGC CAAGGGCTCC TTCACCCCCC 
ACCACCTATG GGTACATCAG CGTCCCAACA GCCTCAGAGT TCACGGACAT GGGCAGGACT 
GGAGGAGGGG TGGGGCCCAA GGGGGGAGTC TTGCTGTGCC CACCTCGGCC CTGCCTCACC 
CCCACCCCCA GCGAGGGCTC CTTAGCCAAT GGTTGGGGCT CAGCCTCTGA GGACAATGCC 
GCCAGCGCCA GAGCCAGCCT TGTCAGCTCC TCCGATGGCT CCTTCCTCGC TGATGCTCAC 
TTTGCCCGGG CCCTGGCAGT GGCTGTGGAT AGCTTTGGTT TCGGTCTAGA GCCCAGGGAG 
GCAGACTGCG TCTTCATAGA TGCCTCATCA CCTCCCTCCC CACGGGATGA GATCTTCCTG 
ACCCCCAACC TCTCCCTGCC CCTGTGGGAG TGGAGGCCAG ACTGGTTGGA AGACATGGAG 
GTCAGCCACA CCCAGCGGCT GGGAAGGGGG ATGCCTCCCT GGCCCCCTGA CTCTCAGATC 
TCTTCCCAGA GAAGTCAGCT CCACTGTCGT ATGCCCAAGG CTGGTGCTTC TCCTGTAGAT 
TACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO4 ( XM_011542875.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541177.1 Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant X2, mRNA.

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CloneID	OHu68437
Clone ID Related Accession (Same CDS sequence)	XM_011542875.1
Accession Version	XM_011542875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGAG CCACCCAAGA ACCCAGTGAG CATGGTCCCT GGACCCTGGA GCAGCTGAGG 
GCTACCTTGA AGCGGCCTGA GGTCATTGCC ACCTGCGGTG TTGCACTCTG GCTGCTGCTT 
CTGGGCACCG CCGTGTGTAT CCACCGCCGG CGCCGAGCTA GGGTGCACCT GGGCCCAGGT 
CTGTACAGAT ATACCAGTGA GGATGCCATC CTAAAACACA GGATGGATCA CAGTGACTCC 
CAGTGGTTGG CAGACACTTG GCGTTCCACC TCTGGCTCTC GGGACCTGAG CAGCAGCAGC 
AGCCTCAGCA GTCGGCTGGG GGCGGATGCC CGGGACCCAC TAGACTGTCG TCGCTCCTTG 
CTCTCCTGGG ACTCCCGAAG CCCCGGCGTG CCCCTGCTTC CAGACACCAG CACTTTTTAT 
GGCTCCCTCA TCGCTGAGCT GCCCTCCAGT ACCCCAGCCA GGCCAAGTCC CCAGGTCCCA 
GCTGTCAGGC GCCTCCCACC CCAGCTGGCC CAGCTCTCCA GCCCCTGTTC CAGCTCAGAC 
AGCCTCTGCA GCCGCAGGGG ACTCTCTTCT CCCCGCTTGT CTCTGGCCCC TGCAGAGGCT 
TGGAAGGCCA AAAAGAAGCA GGAGCTGCAG CATGCCAACA GTTCCCCACT GCTCCGGGGC 
AGCCACTCCT TGGAGCTCCG GGCCTGTGAG TTAGGAAATA GAGGTTCCAA GAACCTTTCC 
CAAAGCCCAG GAGCTGTGCC CCAAGCTCTG GTTGCCTGGC GGGCCCTGGG ACCGAAACTC 
CTCAGCTCCT CAAATGAGCT GGTTACTCGT CATCTCCCTC CAGCACCCCT CTTTCCTCAT 
GAAACTCCCC CAACTCAGAG TCAACAGACC CAGCCTCCGG TGGCACCACA GGCTCCCTCC 
TCCATCCTGC TGCCAGCAGC CCCCATCCCC ATCCTTAGCC CCTGCAGTCC CCCTAGCCCC 
CAGGCCTCTT CCCTCTCTGG CCCCAGCCCA GCTTCCAGTC GCCTGTCCAG CTCCTCACTG 
TCATCCCTGG GGGAGGATCA AGACAGCGTG CTGACCCCTG AGGAGGTAGC CCTGTGCTTG 
GAACTCAGTG AGGGTGAGGA GACTCCCAGG AACAGCGTCT CTCCCATGCC AAGGGCTCCT 
TCACCCCCCA CCACCTATGG GTACATCAGC GTCCCAACAG CCTCAGAGTT CACGGACATG 
GGCAGGACTG GAGGAGGGGT GGGGCCCAAG GGGGGAGTCT TGCTGTGCCC ACCTCGGCCC 
TGCCTCACCC CCACCCCCAG CGAGGGCTCC TTAGCCAATG GTTGGGGCTC AGCCTCTGAG 
GACAATGCCG CCAGCGCCAG AGCCAGCCTT GTCAGCTCCT CCGATGGCTC CTTCCTCGCT 
GATGCTCACT TTGCCCGGGC CCTGGCAGTG GCTGTGGATA GCTTTGGTTT CGGTCTAGAG 
CCCAGGGAGG CAGACTGCGT CTTCATAGAT GCCTCATCAC CTCCCTCCCC ACGGGATGAG 
ATCTTCCTGA CCCCCAACCT CTCCCTGCCC CTGTGGGAGT GGAGGCCAGA CTGGTTGGAA 
GACATGGAGG TCAGCCACAC CCAGCGGCTG GGAAGGGGGA TGCCTCCCTG GCCCCCTGAC 
TCTCAGATCT CTTCCCAGAG AAGTCAGCTC CACTGTCGTA TGCCCAAGGC TGGTGCTTCT 
CCTGTAGATT ACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541177.1
CDS	229..1926
Translation	MERATQEPSEHGPWTLEQLRATLKRPEVIATCGVALWLLLLGTAVCIHRRRRARVHLGPGLYRYTSEDAILKHRMDHSDSQWLADTWRSTSGSRDLSSSSSLSSRLGADARDPLDCRRSLLSWDSRSPGVPLLPDTSTFYGSLIAELPSSTPARPSPQVPAVRRLPPQLAQLSSPCSSSDSLCSRRGLSSPRLSLAPAEAWKAKKKQELQHANSSPLLRGSHSLELRACELGNRGSKNLSQSPGAVPQALVAWRALGPKLLSSSNELVTRHLPPAPLFPHETPPTQSQQTQPPVAPQAPSSILLPAAPIPILSPCSPPSPQASSLSGPSPASSRLSSSSLSSLGEDQDSVLTPEEVALCLELSEGEETPRNSVSPMPRAPSPPTTYGYISVPTASEFTDMGRTGGGVGPKGGVLLCPPRPCLTPTPSEGSLANGWGSASEDNAASARASLVSSSDGSFLADAHFARALAVAVDSFGFGLEPREADCVFIDASSPPSPRDEIFLTPNLSLPLWEWRPDWLEDMEVSHTQRLGRGMPPWPPDSQISSQRSQLHCRMPKAGASPVDYS

Target ORF information:

RefSeq Version	XM_011542875.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542875.1

ORF Insert Sequence:

ATGGAGCGAG CCACCCAAGA ACCCAGTGAG CATGGTCCCT GGACCCTGGA GCAGCTGAGG 
GCTACCTTGA AGCGGCCTGA GGTCATTGCC ACCTGCGGTG TTGCACTCTG GCTGCTGCTT 
CTGGGCACCG CCGTGTGTAT CCACCGCCGG CGCCGAGCTA GGGTGCACCT GGGCCCAGGT 
CTGTACAGAT ATACCAGTGA GGATGCCATC CTAAAACACA GGATGGATCA CAGTGACTCC 
CAGTGGTTGG CAGACACTTG GCGTTCCACC TCTGGCTCTC GGGACCTGAG CAGCAGCAGC 
AGCCTCAGCA GTCGGCTGGG GGCGGATGCC CGGGACCCAC TAGACTGTCG TCGCTCCTTG 
CTCTCCTGGG ACTCCCGAAG CCCCGGCGTG CCCCTGCTTC CAGACACCAG CACTTTTTAT 
GGCTCCCTCA TCGCTGAGCT GCCCTCCAGT ACCCCAGCCA GGCCAAGTCC CCAGGTCCCA 
GCTGTCAGGC GCCTCCCACC CCAGCTGGCC CAGCTCTCCA GCCCCTGTTC CAGCTCAGAC 
AGCCTCTGCA GCCGCAGGGG ACTCTCTTCT CCCCGCTTGT CTCTGGCCCC TGCAGAGGCT 
TGGAAGGCCA AAAAGAAGCA GGAGCTGCAG CATGCCAACA GTTCCCCACT GCTCCGGGGC 
AGCCACTCCT TGGAGCTCCG GGCCTGTGAG TTAGGAAATA GAGGTTCCAA GAACCTTTCC 
CAAAGCCCAG GAGCTGTGCC CCAAGCTCTG GTTGCCTGGC GGGCCCTGGG ACCGAAACTC 
CTCAGCTCCT CAAATGAGCT GGTTACTCGT CATCTCCCTC CAGCACCCCT CTTTCCTCAT 
GAAACTCCCC CAACTCAGAG TCAACAGACC CAGCCTCCGG TGGCACCACA GGCTCCCTCC 
TCCATCCTGC TGCCAGCAGC CCCCATCCCC ATCCTTAGCC CCTGCAGTCC CCCTAGCCCC 
CAGGCCTCTT CCCTCTCTGG CCCCAGCCCA GCTTCCAGTC GCCTGTCCAG CTCCTCACTG 
TCATCCCTGG GGGAGGATCA AGACAGCGTG CTGACCCCTG AGGAGGTAGC CCTGTGCTTG 
GAACTCAGTG AGGGTGAGGA GACTCCCAGG AACAGCGTCT CTCCCATGCC AAGGGCTCCT 
TCACCCCCCA CCACCTATGG GTACATCAGC GTCCCAACAG CCTCAGAGTT CACGGACATG 
GGCAGGACTG GAGGAGGGGT GGGGCCCAAG GGGGGAGTCT TGCTGTGCCC ACCTCGGCCC 
TGCCTCACCC CCACCCCCAG CGAGGGCTCC TTAGCCAATG GTTGGGGCTC AGCCTCTGAG 
GACAATGCCG CCAGCGCCAG AGCCAGCCTT GTCAGCTCCT CCGATGGCTC CTTCCTCGCT 
GATGCTCACT TTGCCCGGGC CCTGGCAGTG GCTGTGGATA GCTTTGGTTT CGGTCTAGAG 
CCCAGGGAGG CAGACTGCGT CTTCATAGAT GCCTCATCAC CTCCCTCCCC ACGGGATGAG 
ATCTTCCTGA CCCCCAACCT CTCCCTGCCC CTGTGGGAGT GGAGGCCAGA CTGGTTGGAA 
GACATGGAGG TCAGCCACAC CCAGCGGCTG GGAAGGGGGA TGCCTCCCTG GCCCCCTGAC 
TCTCAGATCT CTTCCCAGAG AAGTCAGCTC CACTGTCGTA TGCCCAAGGC TGGTGCTTCT 
CCTGTAGATT ACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO4 ( XM_006718861.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718924.1 Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA.

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CloneID	OHu45804
Clone ID Related Accession (Same CDS sequence)	XM_006718861.1 , XM_006718861.2
Accession Version	XM_006718861.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	roundabout homolog 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTG GAGGAGACAG CCTCCTGGGG GGCAGGGGTT CCCTGCCTCT GCTGCTCCTG 
CTCATCATGG GAGGCATGGC TCAGGACTCC CCGCCCCAGA TCCTAGTCCA CCCCCAGGAC 
CAGCTGTTCC AGGGCCCTGG CCCTGCCAGG ATGAGCTGCC AAGCCTCAGG CCAGCCACCT 
CCCACCATCC GCTGGTTGCT GAATGGGCAG CCCCTGAGCA TGGTGCCCCC AGACCCACAC 
CACCTCCTGC CTGATGGGAC CCTTCTGCTG CTACAGCCCC CTGCCCGGGG ACATGCCCAC 
GATGGCCAGG CCCTGTCCAC AGACCTGGGT GTCTACACAT GTGAGGCCAG CAACCGGCTT 
GGCACGGCAG TCAGCAGAGG CGCTCGGCTG TCTGTGGCTG TCCTCCGGGA GGATTTCCAG 
ATCCAGCCTC GGGACATGGT GGCTGTGGTG GGTGAGCAGT TTACTCTGGA ATGTGGGCCG 
CCCTGGGGCC ACCCAGAGCC CACAGTCTCA TGGTGGAAAG ATGGGAAACC CCTGGCCCTC 
CAGCCCGGAA GGCACACAGT GTCCGGGGGG TCCCTGCTGA TGGCAAGAGC AGAGAAGAGT 
GACGAAGGGA CCTACATGTG TGTGGCCACC AACAGCGCAG GACATAGGGA GAGCCGCGCA 
GCCCGGGTTT CCATCCAGGA GCCCCAGGAC TACACGGAGC CTGTGGAGCT TCTGGCTGTG 
CGAATTCAGC TGGAAAATGT GACACTGCTG AACCCGGATC CTGCAGAGGG CCCCAAGCCT 
AGACCGGCGG TGTGGCTCAG CTGGAAGGTC AGTGGCCCTG CTGCGCCTGC CCAATCTTAC 
ACGGCCTTGT TCAGGACCCA GACTGCCCCG GGAGGCCAGG GAGCTCCGTG GGCAGAGGAG 
CTGCTGGCCG GCTGGCAGAG CGCAGAGCTT GGAGGCCTCC ACTGGGGCCA AGACTACGAG 
TTCAAAGTGA GACCATCCTC TGGCCGGGCT CGAGGCCCTG ACAGCAACGT GCTGCTCCTG 
AGGCTGCCGG AAAAAGTGCC CAGTGCCCCA CCTCAGGAAG TGACTCTAAA GCCTGGCAAT 
GGCACTGTCT TTGTGAGCTG GGTCCCACCA CCTGCTGAAA ACCACAATGG CATCATCCGT 
GGCTACCAGG TCTGGAGCCT GGGCAACACA TCACTGCCAC CAGCCAACTG GACTGTAGTT 
GGTGAGCAGA CCCAGCTGGA AATCGCCACC CATATGCCAG GCTCCTACTG CGTGCAAGTG 
GCTGCAGTCA CTGGTGCTGG AGCTGGGGAG CCCAGTAGAC CTGTCTGCCT CCTTTTAGAG 
CAGGCCATGG AGCGAGCCAC CCAAGAACCC AGTGAGCATG GTCCCTGGAC CCTGGAGCAG 
CTGAGGGCTA CCTTGAAGCG GCCTGAGGTC ATTGCCACCT GCGGTGTTGC ACTCTGGCTG 
CTGCTTCTGG GCACCGCCGT GTGTATCCAC CGCCGGCGCC GAGCTAGGGT GCACCTGGGC 
CCAGGTCTGT ACAGATATAC CAGTGAGGAT GCCATCCTAA AACACAGGAT GGATCACAGT 
GACTCCCAGT GGTTGGCAGA CACTTGGCGT TCCACCTCTG GCTCTCGGGA CCTGAGCAGC 
AGCAGCAGCC TCAGCAGTCG GCTGGGGGCG GATGCCCGGG ACCCACTAGA CTGTCGTCGC 
TCCTTGCTCT CCTGGGACTC CCGAAGCCCC GGCGTGCCCC TGCTTCCAGA CACCAGCACT 
TTTTATGGCT CCCTCATCGC TGAGCTGCCC TCCAGTACCC CAGCCAGGCC AAGTCCCCAG 
GTCCCAGCTG TCAGGCGCCT CCCACCCCAG CTGGCCCAGC TCTCCAGCCC CTGTTCCAGC 
TCAGACAGCC TCTGCAGCCG CAGGGGACTC TCTTCTCCCC GCTTGTCTCT GGCCCCTGCA 
GAGGCTTGGA AGGCCAAAAA GAAGCAGGAG CTGCAGCATG CCAACAGTTC CCCACTGCTC 
CGGGGCAGCC ACTCCTTGGA GCTCCGGGCC TGTGAGTTAG GAAATAGAGG TTCCAAGAAC 
CTTTCCCAAA GCCCAGGAGC TGTGCCCCAA GCTCTGGTTG CCTGGCGGGC CCTGGGACCG 
AAACTCCTCA GCTCCTCAAA TGAGCTGGTT ACTCGTCATC TCCCTCCAGC ACCCCTCTTT 
CCTCATGAAA CTCCCCCAAC TCAGAGTCAA CAGACCCACC CAGCTTCCAG TCGCCTGTCC 
AGCTCCTCAC TGTCATCCCT GGGGGAGGAT CAAGACAGCG TGCTGACCCC TGAGGAGGTA 
GCCCTGTGCT TGGAACTCAG TGAGGGTGAG GAGACTCCCA GGAACAGCGT CTCTCCCATG 
CCAAGGGCTC CTTCACCCCC CACCACCTAT GGGTACATCA GCGTCCCAAC AGCCTCAGAG 
TTCACGGACA TGGGCAGGAC TGGAGGAGGG GTGGGGCCCA AGGGGGGAGT CTTGCTGTGC 
CCACCTCGGC CCTGCCTCAC CCCCACCCCC AGCGAGGGCT CCTTAGCCAA TGGTTGGGGC 
TCAGCCTCTG AGGACAATGC CGCCAGCGCC AGAGCCAGCC TTGTCAGCTC CTCCGATGGC 
TCCTTCCTCG CTGATGCTCA CTTTGCCCGG GCCCTGGCAG TGGCTGTGGA TAGCTTTGGT 
TTCGGTCTAG AGCCCAGGGA GGCAGACTGC GTCTTCATAG ATGCCTCATC ACCTCCCTCC 
CCACGGGATG AGATCTTCCT GACCCCCAAC CTCTCCCTGC CCCTGTGGGA GTGGAGGCCA 
GACTGGTTGG AAGACATGGA GGTCAGCCAC ACCCAGCGGC TGGGAAGGGG GATGCCTCCC 
TGGCCCCCTG ACTCTCAGAT CTCTTCCCAG AGAAGTCAGC TCCACTGTCG TATGCCCAAG 
GCTGGTGCTT CTCCTGTAGA TTACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718924.1
CDS	74..2983
Translation	MGSGGDSLLGGRGSLPLLLLLIMGGMAQDSPPQILVHPQDQLFQGPGPARMSCQASGQPPPTIRWLLNGQPLSMVPPDPHHLLPDGTLLLLQPPARGHAHDGQALSTDLGVYTCEASNRLGTAVSRGARLSVAVLREDFQIQPRDMVAVVGEQFTLECGPPWGHPEPTVSWWKDGKPLALQPGRHTVSGGSLLMARAEKSDEGTYMCVATNSAGHRESRAARVSIQEPQDYTEPVELLAVRIQLENVTLLNPDPAEGPKPRPAVWLSWKVSGPAAPAQSYTALFRTQTAPGGQGAPWAEELLAGWQSAELGGLHWGQDYEFKVRPSSGRARGPDSNVLLLRLPEKVPSAPPQEVTLKPGNGTVFVSWVPPPAENHNGIIRGYQVWSLGNTSLPPANWTVVGEQTQLEIATHMPGSYCVQVAAVTGAGAGEPSRPVCLLLEQAMERATQEPSEHGPWTLEQLRATLKRPEVIATCGVALWLLLLGTAVCIHRRRRARVHLGPGLYRYTSEDAILKHRMDHSDSQWLADTWRSTSGSRDLSSSSSLSSRLGADARDPLDCRRSLLSWDSRSPGVPLLPDTSTFYGSLIAELPSSTPARPSPQVPAVRRLPPQLAQLSSPCSSSDSLCSRRGLSSPRLSLAPAEAWKAKKKQELQHANSSPLLRGSHSLELRACELGNRGSKNLSQSPGAVPQALVAWRALGPKLLSSSNELVTRHLPPAPLFPHETPPTQSQQTHPASSRLSSSSLSSLGEDQDSVLTPEEVALCLELSEGEETPRNSVSPMPRAPSPPTTYGYISVPTASEFTDMGRTGGGVGPKGGVLLCPPRPCLTPTPSEGSLANGWGSASEDNAASARASLVSSSDGSFLADAHFARALAVAVDSFGFGLEPREADCVFIDASSPPSPRDEIFLTPNLSLPLWEWRPDWLEDMEVSHTQRLGRGMPPWPPDSQISSQRSQLHCRMPKAGASPVDYS

Target ORF information:

RefSeq Version	XM_006718861.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718861.1

ORF Insert Sequence:

ATGGGCTCTG GAGGAGACAG CCTCCTGGGG GGCAGGGGTT CCCTGCCTCT GCTGCTCCTG 
CTCATCATGG GAGGCATGGC TCAGGACTCC CCGCCCCAGA TCCTAGTCCA CCCCCAGGAC 
CAGCTGTTCC AGGGCCCTGG CCCTGCCAGG ATGAGCTGCC AAGCCTCAGG CCAGCCACCT 
CCCACCATCC GCTGGTTGCT GAATGGGCAG CCCCTGAGCA TGGTGCCCCC AGACCCACAC 
CACCTCCTGC CTGATGGGAC CCTTCTGCTG CTACAGCCCC CTGCCCGGGG ACATGCCCAC 
GATGGCCAGG CCCTGTCCAC AGACCTGGGT GTCTACACAT GTGAGGCCAG CAACCGGCTT 
GGCACGGCAG TCAGCAGAGG CGCTCGGCTG TCTGTGGCTG TCCTCCGGGA GGATTTCCAG 
ATCCAGCCTC GGGACATGGT GGCTGTGGTG GGTGAGCAGT TTACTCTGGA ATGTGGGCCG 
CCCTGGGGCC ACCCAGAGCC CACAGTCTCA TGGTGGAAAG ATGGGAAACC CCTGGCCCTC 
CAGCCCGGAA GGCACACAGT GTCCGGGGGG TCCCTGCTGA TGGCAAGAGC AGAGAAGAGT 
GACGAAGGGA CCTACATGTG TGTGGCCACC AACAGCGCAG GACATAGGGA GAGCCGCGCA 
GCCCGGGTTT CCATCCAGGA GCCCCAGGAC TACACGGAGC CTGTGGAGCT TCTGGCTGTG 
CGAATTCAGC TGGAAAATGT GACACTGCTG AACCCGGATC CTGCAGAGGG CCCCAAGCCT 
AGACCGGCGG TGTGGCTCAG CTGGAAGGTC AGTGGCCCTG CTGCGCCTGC CCAATCTTAC 
ACGGCCTTGT TCAGGACCCA GACTGCCCCG GGAGGCCAGG GAGCTCCGTG GGCAGAGGAG 
CTGCTGGCCG GCTGGCAGAG CGCAGAGCTT GGAGGCCTCC ACTGGGGCCA AGACTACGAG 
TTCAAAGTGA GACCATCCTC TGGCCGGGCT CGAGGCCCTG ACAGCAACGT GCTGCTCCTG 
AGGCTGCCGG AAAAAGTGCC CAGTGCCCCA CCTCAGGAAG TGACTCTAAA GCCTGGCAAT 
GGCACTGTCT TTGTGAGCTG GGTCCCACCA CCTGCTGAAA ACCACAATGG CATCATCCGT 
GGCTACCAGG TCTGGAGCCT GGGCAACACA TCACTGCCAC CAGCCAACTG GACTGTAGTT 
GGTGAGCAGA CCCAGCTGGA AATCGCCACC CATATGCCAG GCTCCTACTG CGTGCAAGTG 
GCTGCAGTCA CTGGTGCTGG AGCTGGGGAG CCCAGTAGAC CTGTCTGCCT CCTTTTAGAG 
CAGGCCATGG AGCGAGCCAC CCAAGAACCC AGTGAGCATG GTCCCTGGAC CCTGGAGCAG 
CTGAGGGCTA CCTTGAAGCG GCCTGAGGTC ATTGCCACCT GCGGTGTTGC ACTCTGGCTG 
CTGCTTCTGG GCACCGCCGT GTGTATCCAC CGCCGGCGCC GAGCTAGGGT GCACCTGGGC 
CCAGGTCTGT ACAGATATAC CAGTGAGGAT GCCATCCTAA AACACAGGAT GGATCACAGT 
GACTCCCAGT GGTTGGCAGA CACTTGGCGT TCCACCTCTG GCTCTCGGGA CCTGAGCAGC 
AGCAGCAGCC TCAGCAGTCG GCTGGGGGCG GATGCCCGGG ACCCACTAGA CTGTCGTCGC 
TCCTTGCTCT CCTGGGACTC CCGAAGCCCC GGCGTGCCCC TGCTTCCAGA CACCAGCACT 
TTTTATGGCT CCCTCATCGC TGAGCTGCCC TCCAGTACCC CAGCCAGGCC AAGTCCCCAG 
GTCCCAGCTG TCAGGCGCCT CCCACCCCAG CTGGCCCAGC TCTCCAGCCC CTGTTCCAGC 
TCAGACAGCC TCTGCAGCCG CAGGGGACTC TCTTCTCCCC GCTTGTCTCT GGCCCCTGCA 
GAGGCTTGGA AGGCCAAAAA GAAGCAGGAG CTGCAGCATG CCAACAGTTC CCCACTGCTC 
CGGGGCAGCC ACTCCTTGGA GCTCCGGGCC TGTGAGTTAG GAAATAGAGG TTCCAAGAAC 
CTTTCCCAAA GCCCAGGAGC TGTGCCCCAA GCTCTGGTTG CCTGGCGGGC CCTGGGACCG 
AAACTCCTCA GCTCCTCAAA TGAGCTGGTT ACTCGTCATC TCCCTCCAGC ACCCCTCTTT 
CCTCATGAAA CTCCCCCAAC TCAGAGTCAA CAGACCCACC CAGCTTCCAG TCGCCTGTCC 
AGCTCCTCAC TGTCATCCCT GGGGGAGGAT CAAGACAGCG TGCTGACCCC TGAGGAGGTA 
GCCCTGTGCT TGGAACTCAG TGAGGGTGAG GAGACTCCCA GGAACAGCGT CTCTCCCATG 
CCAAGGGCTC CTTCACCCCC CACCACCTAT GGGTACATCA GCGTCCCAAC AGCCTCAGAG 
TTCACGGACA TGGGCAGGAC TGGAGGAGGG GTGGGGCCCA AGGGGGGAGT CTTGCTGTGC 
CCACCTCGGC CCTGCCTCAC CCCCACCCCC AGCGAGGGCT CCTTAGCCAA TGGTTGGGGC 
TCAGCCTCTG AGGACAATGC CGCCAGCGCC AGAGCCAGCC TTGTCAGCTC CTCCGATGGC 
TCCTTCCTCG CTGATGCTCA CTTTGCCCGG GCCCTGGCAG TGGCTGTGGA TAGCTTTGGT 
TTCGGTCTAG AGCCCAGGGA GGCAGACTGC GTCTTCATAG ATGCCTCATC ACCTCCCTCC 
CCACGGGATG AGATCTTCCT GACCCCCAAC CTCTCCCTGC CCCTGTGGGA GTGGAGGCCA 
GACTGGTTGG AAGACATGGA GGTCAGCCAC ACCCAGCGGC TGGGAAGGGG GATGCCTCCC 
TGGCCCCCTG ACTCTCAGAT CTCTTCCCAG AGAAGTCAGC TCCACTGTCG TATGCCCAAG 
GCTGGTGCTT CTCCTGTAGA TTACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO4 ( XM_006718861.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718924.1 Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA.

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CloneID	OHu45804
Clone ID Related Accession (Same CDS sequence)	XM_006718861.1 , XM_006718861.2
Accession Version	XM_006718861.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006718861.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTG GAGGAGACAG CCTCCTGGGG GGCAGGGGTT CCCTGCCTCT GCTGCTCCTG 
CTCATCATGG GAGGCATGGC TCAGGACTCC CCGCCCCAGA TCCTAGTCCA CCCCCAGGAC 
CAGCTGTTCC AGGGCCCTGG CCCTGCCAGG ATGAGCTGCC AAGCCTCAGG CCAGCCACCT 
CCCACCATCC GCTGGTTGCT GAATGGGCAG CCCCTGAGCA TGGTGCCCCC AGACCCACAC 
CACCTCCTGC CTGATGGGAC CCTTCTGCTG CTACAGCCCC CTGCCCGGGG ACATGCCCAC 
GATGGCCAGG CCCTGTCCAC AGACCTGGGT GTCTACACAT GTGAGGCCAG CAACCGGCTT 
GGCACGGCAG TCAGCAGAGG CGCTCGGCTG TCTGTGGCTG TCCTCCGGGA GGATTTCCAG 
ATCCAGCCTC GGGACATGGT GGCTGTGGTG GGTGAGCAGT TTACTCTGGA ATGTGGGCCG 
CCCTGGGGCC ACCCAGAGCC CACAGTCTCA TGGTGGAAAG ATGGGAAACC CCTGGCCCTC 
CAGCCCGGAA GGCACACAGT GTCCGGGGGG TCCCTGCTGA TGGCAAGAGC AGAGAAGAGT 
GACGAAGGGA CCTACATGTG TGTGGCCACC AACAGCGCAG GACATAGGGA GAGCCGCGCA 
GCCCGGGTTT CCATCCAGGA GCCCCAGGAC TACACGGAGC CTGTGGAGCT TCTGGCTGTG 
CGAATTCAGC TGGAAAATGT GACACTGCTG AACCCGGATC CTGCAGAGGG CCCCAAGCCT 
AGACCGGCGG TGTGGCTCAG CTGGAAGGTC AGTGGCCCTG CTGCGCCTGC CCAATCTTAC 
ACGGCCTTGT TCAGGACCCA GACTGCCCCG GGAGGCCAGG GAGCTCCGTG GGCAGAGGAG 
CTGCTGGCCG GCTGGCAGAG CGCAGAGCTT GGAGGCCTCC ACTGGGGCCA AGACTACGAG 
TTCAAAGTGA GACCATCCTC TGGCCGGGCT CGAGGCCCTG ACAGCAACGT GCTGCTCCTG 
AGGCTGCCGG AAAAAGTGCC CAGTGCCCCA CCTCAGGAAG TGACTCTAAA GCCTGGCAAT 
GGCACTGTCT TTGTGAGCTG GGTCCCACCA CCTGCTGAAA ACCACAATGG CATCATCCGT 
GGCTACCAGG TCTGGAGCCT GGGCAACACA TCACTGCCAC CAGCCAACTG GACTGTAGTT 
GGTGAGCAGA CCCAGCTGGA AATCGCCACC CATATGCCAG GCTCCTACTG CGTGCAAGTG 
GCTGCAGTCA CTGGTGCTGG AGCTGGGGAG CCCAGTAGAC CTGTCTGCCT CCTTTTAGAG 
CAGGCCATGG AGCGAGCCAC CCAAGAACCC AGTGAGCATG GTCCCTGGAC CCTGGAGCAG 
CTGAGGGCTA CCTTGAAGCG GCCTGAGGTC ATTGCCACCT GCGGTGTTGC ACTCTGGCTG 
CTGCTTCTGG GCACCGCCGT GTGTATCCAC CGCCGGCGCC GAGCTAGGGT GCACCTGGGC 
CCAGGTCTGT ACAGATATAC CAGTGAGGAT GCCATCCTAA AACACAGGAT GGATCACAGT 
GACTCCCAGT GGTTGGCAGA CACTTGGCGT TCCACCTCTG GCTCTCGGGA CCTGAGCAGC 
AGCAGCAGCC TCAGCAGTCG GCTGGGGGCG GATGCCCGGG ACCCACTAGA CTGTCGTCGC 
TCCTTGCTCT CCTGGGACTC CCGAAGCCCC GGCGTGCCCC TGCTTCCAGA CACCAGCACT 
TTTTATGGCT CCCTCATCGC TGAGCTGCCC TCCAGTACCC CAGCCAGGCC AAGTCCCCAG 
GTCCCAGCTG TCAGGCGCCT CCCACCCCAG CTGGCCCAGC TCTCCAGCCC CTGTTCCAGC 
TCAGACAGCC TCTGCAGCCG CAGGGGACTC TCTTCTCCCC GCTTGTCTCT GGCCCCTGCA 
GAGGCTTGGA AGGCCAAAAA GAAGCAGGAG CTGCAGCATG CCAACAGTTC CCCACTGCTC 
CGGGGCAGCC ACTCCTTGGA GCTCCGGGCC TGTGAGTTAG GAAATAGAGG TTCCAAGAAC 
CTTTCCCAAA GCCCAGGAGC TGTGCCCCAA GCTCTGGTTG CCTGGCGGGC CCTGGGACCG 
AAACTCCTCA GCTCCTCAAA TGAGCTGGTT ACTCGTCATC TCCCTCCAGC ACCCCTCTTT 
CCTCATGAAA CTCCCCCAAC TCAGAGTCAA CAGACCCACC CAGCTTCCAG TCGCCTGTCC 
AGCTCCTCAC TGTCATCCCT GGGGGAGGAT CAAGACAGCG TGCTGACCCC TGAGGAGGTA 
GCCCTGTGCT TGGAACTCAG TGAGGGTGAG GAGACTCCCA GGAACAGCGT CTCTCCCATG 
CCAAGGGCTC CTTCACCCCC CACCACCTAT GGGTACATCA GCGTCCCAAC AGCCTCAGAG 
TTCACGGACA TGGGCAGGAC TGGAGGAGGG GTGGGGCCCA AGGGGGGAGT CTTGCTGTGC 
CCACCTCGGC CCTGCCTCAC CCCCACCCCC AGCGAGGGCT CCTTAGCCAA TGGTTGGGGC 
TCAGCCTCTG AGGACAATGC CGCCAGCGCC AGAGCCAGCC TTGTCAGCTC CTCCGATGGC 
TCCTTCCTCG CTGATGCTCA CTTTGCCCGG GCCCTGGCAG TGGCTGTGGA TAGCTTTGGT 
TTCGGTCTAG AGCCCAGGGA GGCAGACTGC GTCTTCATAG ATGCCTCATC ACCTCCCTCC 
CCACGGGATG AGATCTTCCT GACCCCCAAC CTCTCCCTGC CCCTGTGGGA GTGGAGGCCA 
GACTGGTTGG AAGACATGGA GGTCAGCCAC ACCCAGCGGC TGGGAAGGGG GATGCCTCCC 
TGGCCCCCTG ACTCTCAGAT CTCTTCCCAG AGAAGTCAGC TCCACTGTCG TATGCCCAAG 
GCTGGTGCTT CTCCTGTAGA TTACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718924.1
CDS	44..2953
Translation	MGSGGDSLLGGRGSLPLLLLLIMGGMAQDSPPQILVHPQDQLFQGPGPARMSCQASGQPPPTIRWLLNGQPLSMVPPDPHHLLPDGTLLLLQPPARGHAHDGQALSTDLGVYTCEASNRLGTAVSRGARLSVAVLREDFQIQPRDMVAVVGEQFTLECGPPWGHPEPTVSWWKDGKPLALQPGRHTVSGGSLLMARAEKSDEGTYMCVATNSAGHRESRAARVSIQEPQDYTEPVELLAVRIQLENVTLLNPDPAEGPKPRPAVWLSWKVSGPAAPAQSYTALFRTQTAPGGQGAPWAEELLAGWQSAELGGLHWGQDYEFKVRPSSGRARGPDSNVLLLRLPEKVPSAPPQEVTLKPGNGTVFVSWVPPPAENHNGIIRGYQVWSLGNTSLPPANWTVVGEQTQLEIATHMPGSYCVQVAAVTGAGAGEPSRPVCLLLEQAMERATQEPSEHGPWTLEQLRATLKRPEVIATCGVALWLLLLGTAVCIHRRRRARVHLGPGLYRYTSEDAILKHRMDHSDSQWLADTWRSTSGSRDLSSSSSLSSRLGADARDPLDCRRSLLSWDSRSPGVPLLPDTSTFYGSLIAELPSSTPARPSPQVPAVRRLPPQLAQLSSPCSSSDSLCSRRGLSSPRLSLAPAEAWKAKKKQELQHANSSPLLRGSHSLELRACELGNRGSKNLSQSPGAVPQALVAWRALGPKLLSSSNELVTRHLPPAPLFPHETPPTQSQQTHPASSRLSSSSLSSLGEDQDSVLTPEEVALCLELSEGEETPRNSVSPMPRAPSPPTTYGYISVPTASEFTDMGRTGGGVGPKGGVLLCPPRPCLTPTPSEGSLANGWGSASEDNAASARASLVSSSDGSFLADAHFARALAVAVDSFGFGLEPREADCVFIDASSPPSPRDEIFLTPNLSLPLWEWRPDWLEDMEVSHTQRLGRGMPPWPPDSQISSQRSQLHCRMPKAGASPVDYS

Target ORF information:

RefSeq Version	XM_006718861.2
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718861.2

ORF Insert Sequence:

ATGGGCTCTG GAGGAGACAG CCTCCTGGGG GGCAGGGGTT CCCTGCCTCT GCTGCTCCTG 
CTCATCATGG GAGGCATGGC TCAGGACTCC CCGCCCCAGA TCCTAGTCCA CCCCCAGGAC 
CAGCTGTTCC AGGGCCCTGG CCCTGCCAGG ATGAGCTGCC AAGCCTCAGG CCAGCCACCT 
CCCACCATCC GCTGGTTGCT GAATGGGCAG CCCCTGAGCA TGGTGCCCCC AGACCCACAC 
CACCTCCTGC CTGATGGGAC CCTTCTGCTG CTACAGCCCC CTGCCCGGGG ACATGCCCAC 
GATGGCCAGG CCCTGTCCAC AGACCTGGGT GTCTACACAT GTGAGGCCAG CAACCGGCTT 
GGCACGGCAG TCAGCAGAGG CGCTCGGCTG TCTGTGGCTG TCCTCCGGGA GGATTTCCAG 
ATCCAGCCTC GGGACATGGT GGCTGTGGTG GGTGAGCAGT TTACTCTGGA ATGTGGGCCG 
CCCTGGGGCC ACCCAGAGCC CACAGTCTCA TGGTGGAAAG ATGGGAAACC CCTGGCCCTC 
CAGCCCGGAA GGCACACAGT GTCCGGGGGG TCCCTGCTGA TGGCAAGAGC AGAGAAGAGT 
GACGAAGGGA CCTACATGTG TGTGGCCACC AACAGCGCAG GACATAGGGA GAGCCGCGCA 
GCCCGGGTTT CCATCCAGGA GCCCCAGGAC TACACGGAGC CTGTGGAGCT TCTGGCTGTG 
CGAATTCAGC TGGAAAATGT GACACTGCTG AACCCGGATC CTGCAGAGGG CCCCAAGCCT 
AGACCGGCGG TGTGGCTCAG CTGGAAGGTC AGTGGCCCTG CTGCGCCTGC CCAATCTTAC 
ACGGCCTTGT TCAGGACCCA GACTGCCCCG GGAGGCCAGG GAGCTCCGTG GGCAGAGGAG 
CTGCTGGCCG GCTGGCAGAG CGCAGAGCTT GGAGGCCTCC ACTGGGGCCA AGACTACGAG 
TTCAAAGTGA GACCATCCTC TGGCCGGGCT CGAGGCCCTG ACAGCAACGT GCTGCTCCTG 
AGGCTGCCGG AAAAAGTGCC CAGTGCCCCA CCTCAGGAAG TGACTCTAAA GCCTGGCAAT 
GGCACTGTCT TTGTGAGCTG GGTCCCACCA CCTGCTGAAA ACCACAATGG CATCATCCGT 
GGCTACCAGG TCTGGAGCCT GGGCAACACA TCACTGCCAC CAGCCAACTG GACTGTAGTT 
GGTGAGCAGA CCCAGCTGGA AATCGCCACC CATATGCCAG GCTCCTACTG CGTGCAAGTG 
GCTGCAGTCA CTGGTGCTGG AGCTGGGGAG CCCAGTAGAC CTGTCTGCCT CCTTTTAGAG 
CAGGCCATGG AGCGAGCCAC CCAAGAACCC AGTGAGCATG GTCCCTGGAC CCTGGAGCAG 
CTGAGGGCTA CCTTGAAGCG GCCTGAGGTC ATTGCCACCT GCGGTGTTGC ACTCTGGCTG 
CTGCTTCTGG GCACCGCCGT GTGTATCCAC CGCCGGCGCC GAGCTAGGGT GCACCTGGGC 
CCAGGTCTGT ACAGATATAC CAGTGAGGAT GCCATCCTAA AACACAGGAT GGATCACAGT 
GACTCCCAGT GGTTGGCAGA CACTTGGCGT TCCACCTCTG GCTCTCGGGA CCTGAGCAGC 
AGCAGCAGCC TCAGCAGTCG GCTGGGGGCG GATGCCCGGG ACCCACTAGA CTGTCGTCGC 
TCCTTGCTCT CCTGGGACTC CCGAAGCCCC GGCGTGCCCC TGCTTCCAGA CACCAGCACT 
TTTTATGGCT CCCTCATCGC TGAGCTGCCC TCCAGTACCC CAGCCAGGCC AAGTCCCCAG 
GTCCCAGCTG TCAGGCGCCT CCCACCCCAG CTGGCCCAGC TCTCCAGCCC CTGTTCCAGC 
TCAGACAGCC TCTGCAGCCG CAGGGGACTC TCTTCTCCCC GCTTGTCTCT GGCCCCTGCA 
GAGGCTTGGA AGGCCAAAAA GAAGCAGGAG CTGCAGCATG CCAACAGTTC CCCACTGCTC 
CGGGGCAGCC ACTCCTTGGA GCTCCGGGCC TGTGAGTTAG GAAATAGAGG TTCCAAGAAC 
CTTTCCCAAA GCCCAGGAGC TGTGCCCCAA GCTCTGGTTG CCTGGCGGGC CCTGGGACCG 
AAACTCCTCA GCTCCTCAAA TGAGCTGGTT ACTCGTCATC TCCCTCCAGC ACCCCTCTTT 
CCTCATGAAA CTCCCCCAAC TCAGAGTCAA CAGACCCACC CAGCTTCCAG TCGCCTGTCC 
AGCTCCTCAC TGTCATCCCT GGGGGAGGAT CAAGACAGCG TGCTGACCCC TGAGGAGGTA 
GCCCTGTGCT TGGAACTCAG TGAGGGTGAG GAGACTCCCA GGAACAGCGT CTCTCCCATG 
CCAAGGGCTC CTTCACCCCC CACCACCTAT GGGTACATCA GCGTCCCAAC AGCCTCAGAG 
TTCACGGACA TGGGCAGGAC TGGAGGAGGG GTGGGGCCCA AGGGGGGAGT CTTGCTGTGC 
CCACCTCGGC CCTGCCTCAC CCCCACCCCC AGCGAGGGCT CCTTAGCCAA TGGTTGGGGC 
TCAGCCTCTG AGGACAATGC CGCCAGCGCC AGAGCCAGCC TTGTCAGCTC CTCCGATGGC 
TCCTTCCTCG CTGATGCTCA CTTTGCCCGG GCCCTGGCAG TGGCTGTGGA TAGCTTTGGT 
TTCGGTCTAG AGCCCAGGGA GGCAGACTGC GTCTTCATAG ATGCCTCATC ACCTCCCTCC 
CCACGGGATG AGATCTTCCT GACCCCCAAC CTCTCCCTGC CCCTGTGGGA GTGGAGGCCA 
GACTGGTTGG AAGACATGGA GGTCAGCCAC ACCCAGCGGC TGGGAAGGGG GATGCCTCCC 
TGGCCCCCTG ACTCTCAGAT CTCTTCCCAG AGAAGTCAGC TCCACTGTCG TATGCCCAAG 
GCTGGTGCTT CTCCTGTAGA TTACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO4 ( NM_019055.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_061928.4 Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant 1, mRNA.

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CloneID	OHu09210
Clone ID Related Accession (Same CDS sequence)	NM_019055.6 , NM_019055.5
Accession Version	NM_019055.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	roundabout homolog 4 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC424813.1, AF361473.2 and AP003501.2. On May 17, 2019 this sequence version replaced NM_019055.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK027852.1, AF361473.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000306534.8/ ENSP00000304945.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCTCTG GAGGAGACAG CCTCCTGGGG GGCAGGGGTT CCCTGCCTCT GCTGCTCCTG 
CTCATCATGG GAGGCATGGC TCAGGACTCC CCGCCCCAGA TCCTAGTCCA CCCCCAGGAC 
CAGCTGTTCC AGGGCCCTGG CCCTGCCAGG ATGAGCTGCC AAGCCTCAGG CCAGCCACCT 
CCCACCATCC GCTGGTTGCT GAATGGGCAG CCCCTGAGCA TGGTGCCCCC AGACCCACAC 
CACCTCCTGC CTGATGGGAC CCTTCTGCTG CTACAGCCCC CTGCCCGGGG ACATGCCCAC 
GATGGCCAGG CCCTGTCCAC AGACCTGGGT GTCTACACAT GTGAGGCCAG CAACCGGCTT 
GGCACGGCAG TCAGCAGAGG CGCTCGGCTG TCTGTGGCTG TCCTCCGGGA GGATTTCCAG 
ATCCAGCCTC GGGACATGGT GGCTGTGGTG GGTGAGCAGT TTACTCTGGA ATGTGGGCCG 
CCCTGGGGCC ACCCAGAGCC CACAGTCTCA TGGTGGAAAG ATGGGAAACC CCTGGCCCTC 
CAGCCCGGAA GGCACACAGT GTCCGGGGGG TCCCTGCTGA TGGCAAGAGC AGAGAAGAGT 
GACGAAGGGA CCTACATGTG TGTGGCCACC AACAGCGCAG GACATAGGGA GAGCCGCGCA 
GCCCGGGTTT CCATCCAGGA GCCCCAGGAC TACACGGAGC CTGTGGAGCT TCTGGCTGTG 
CGAATTCAGC TGGAAAATGT GACACTGCTG AACCCGGATC CTGCAGAGGG CCCCAAGCCT 
AGACCGGCGG TGTGGCTCAG CTGGAAGGTC AGTGGCCCTG CTGCGCCTGC CCAATCTTAC 
ACGGCCTTGT TCAGGACCCA GACTGCCCCG GGAGGCCAGG GAGCTCCGTG GGCAGAGGAG 
CTGCTGGCCG GCTGGCAGAG CGCAGAGCTT GGAGGCCTCC ACTGGGGCCA AGACTACGAG 
TTCAAAGTGA GACCATCCTC TGGCCGGGCT CGAGGCCCTG ACAGCAACGT GCTGCTCCTG 
AGGCTGCCGG AAAAAGTGCC CAGTGCCCCA CCTCAGGAAG TGACTCTAAA GCCTGGCAAT 
GGCACTGTCT TTGTGAGCTG GGTCCCACCA CCTGCTGAAA ACCACAATGG CATCATCCGT 
GGCTACCAGG TCTGGAGCCT GGGCAACACA TCACTGCCAC CAGCCAACTG GACTGTAGTT 
GGTGAGCAGA CCCAGCTGGA AATCGCCACC CATATGCCAG GCTCCTACTG CGTGCAAGTG 
GCTGCAGTCA CTGGTGCTGG AGCTGGGGAG CCCAGTAGAC CTGTCTGCCT CCTTTTAGAG 
CAGGCCATGG AGCGAGCCAC CCAAGAACCC AGTGAGCATG GTCCCTGGAC CCTGGAGCAG 
CTGAGGGCTA CCTTGAAGCG GCCTGAGGTC ATTGCCACCT GCGGTGTTGC ACTCTGGCTG 
CTGCTTCTGG GCACCGCCGT GTGTATCCAC CGCCGGCGCC GAGCTAGGGT GCACCTGGGC 
CCAGGTCTGT ACAGATATAC CAGTGAGGAT GCCATCCTAA AACACAGGAT GGATCACAGT 
GACTCCCAGT GGTTGGCAGA CACTTGGCGT TCCACCTCTG GCTCTCGGGA CCTGAGCAGC 
AGCAGCAGCC TCAGCAGTCG GCTGGGGGCG GATGCCCGGG ACCCACTAGA CTGTCGTCGC 
TCCTTGCTCT CCTGGGACTC CCGAAGCCCC GGCGTGCCCC TGCTTCCAGA CACCAGCACT 
TTTTATGGCT CCCTCATCGC TGAGCTGCCC TCCAGTACCC CAGCCAGGCC AAGTCCCCAG 
GTCCCAGCTG TCAGGCGCCT CCCACCCCAG CTGGCCCAGC TCTCCAGCCC CTGTTCCAGC 
TCAGACAGCC TCTGCAGCCG CAGGGGACTC TCTTCTCCCC GCTTGTCTCT GGCCCCTGCA 
GAGGCTTGGA AGGCCAAAAA GAAGCAGGAG CTGCAGCATG CCAACAGTTC CCCACTGCTC 
CGGGGCAGCC ACTCCTTGGA GCTCCGGGCC TGTGAGTTAG GAAATAGAGG TTCCAAGAAC 
CTTTCCCAAA GCCCAGGAGC TGTGCCCCAA GCTCTGGTTG CCTGGCGGGC CCTGGGACCG 
AAACTCCTCA GCTCCTCAAA TGAGCTGGTT ACTCGTCATC TCCCTCCAGC ACCCCTCTTT 
CCTCATGAAA CTCCCCCAAC TCAGAGTCAA CAGACCCAGC CTCCGGTGGC ACCACAGGCT 
CCCTCCTCCA TCCTGCTGCC AGCAGCCCCC ATCCCCATCC TTAGCCCCTG CAGTCCCCCT 
AGCCCCCAGG CCTCTTCCCT CTCTGGCCCC AGCCCAGCTT CCAGTCGCCT GTCCAGCTCC 
TCACTGTCAT CCCTGGGGGA GGATCAAGAC AGCGTGCTGA CCCCTGAGGA GGTAGCCCTG 
TGCTTGGAAC TCAGTGAGGG TGAGGAGACT CCCAGGAACA GCGTCTCTCC CATGCCAAGG 
GCTCCTTCAC CCCCCACCAC CTATGGGTAC ATCAGCGTCC CAACAGCCTC AGAGTTCACG 
GACATGGGCA GGACTGGAGG AGGGGTGGGG CCCAAGGGGG GAGTCTTGCT GTGCCCACCT 
CGGCCCTGCC TCACCCCCAC CCCCAGCGAG GGCTCCTTAG CCAATGGTTG GGGCTCAGCC 
TCTGAGGACA ATGCCGCCAG CGCCAGAGCC AGCCTTGTCA GCTCCTCCGA TGGCTCCTTC 
CTCGCTGATG CTCACTTTGC CCGGGCCCTG GCAGTGGCTG TGGATAGCTT TGGTTTCGGT 
CTAGAGCCCA GGGAGGCAGA CTGCGTCTTC ATAGATGCCT CATCACCTCC CTCCCCACGG 
GATGAGATCT TCCTGACCCC CAACCTCTCC CTGCCCCTGT GGGAGTGGAG GCCAGACTGG 
TTGGAAGACA TGGAGGTCAG CCACACCCAG CGGCTGGGAA GGGGGATGCC TCCCTGGCCC 
CCTGACTCTC AGATCTCTTC CCAGAGAAGT CAGCTCCACT GTCGTATGCC CAAGGCTGGT 
GCTTCTCCTG TAGATTACTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061928.4
CDS	71..3094
Translation	MGSGGDSLLGGRGSLPLLLLLIMGGMAQDSPPQILVHPQDQLFQGPGPARMSCQASGQPPPTIRWLLNGQPLSMVPPDPHHLLPDGTLLLLQPPARGHAHDGQALSTDLGVYTCEASNRLGTAVSRGARLSVAVLREDFQIQPRDMVAVVGEQFTLECGPPWGHPEPTVSWWKDGKPLALQPGRHTVSGGSLLMARAEKSDEGTYMCVATNSAGHRESRAARVSIQEPQDYTEPVELLAVRIQLENVTLLNPDPAEGPKPRPAVWLSWKVSGPAAPAQSYTALFRTQTAPGGQGAPWAEELLAGWQSAELGGLHWGQDYEFKVRPSSGRARGPDSNVLLLRLPEKVPSAPPQEVTLKPGNGTVFVSWVPPPAENHNGIIRGYQVWSLGNTSLPPANWTVVGEQTQLEIATHMPGSYCVQVAAVTGAGAGEPSRPVCLLLEQAMERATQEPSEHGPWTLEQLRATLKRPEVIATCGVALWLLLLGTAVCIHRRRRARVHLGPGLYRYTSEDAILKHRMDHSDSQWLADTWRSTSGSRDLSSSSSLSSRLGADARDPLDCRRSLLSWDSRSPGVPLLPDTSTFYGSLIAELPSSTPARPSPQVPAVRRLPPQLAQLSSPCSSSDSLCSRRGLSSPRLSLAPAEAWKAKKKQELQHANSSPLLRGSHSLELRACELGNRGSKNLSQSPGAVPQALVAWRALGPKLLSSSNELVTRHLPPAPLFPHETPPTQSQQTQPPVAPQAPSSILLPAAPIPILSPCSPPSPQASSLSGPSPASSRLSSSSLSSLGEDQDSVLTPEEVALCLELSEGEETPRNSVSPMPRAPSPPTTYGYISVPTASEFTDMGRTGGGVGPKGGVLLCPPRPCLTPTPSEGSLANGWGSASEDNAASARASLVSSSDGSFLADAHFARALAVAVDSFGFGLEPREADCVFIDASSPPSPRDEIFLTPNLSLPLWEWRPDWLEDMEVSHTQRLGRGMPPWPPDSQISSQRSQLHCRMPKAGASPVDYS

Target ORF information:

RefSeq Version	NM_019055.6
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019055.6

ORF Insert Sequence:

ATGGGCTCTG GAGGAGACAG CCTCCTGGGG GGCAGGGGTT CCCTGCCTCT GCTGCTCCTG 
CTCATCATGG GAGGCATGGC TCAGGACTCC CCGCCCCAGA TCCTAGTCCA CCCCCAGGAC 
CAGCTGTTCC AGGGCCCTGG CCCTGCCAGG ATGAGCTGCC AAGCCTCAGG CCAGCCACCT 
CCCACCATCC GCTGGTTGCT GAATGGGCAG CCCCTGAGCA TGGTGCCCCC AGACCCACAC 
CACCTCCTGC CTGATGGGAC CCTTCTGCTG CTACAGCCCC CTGCCCGGGG ACATGCCCAC 
GATGGCCAGG CCCTGTCCAC AGACCTGGGT GTCTACACAT GTGAGGCCAG CAACCGGCTT 
GGCACGGCAG TCAGCAGAGG CGCTCGGCTG TCTGTGGCTG TCCTCCGGGA GGATTTCCAG 
ATCCAGCCTC GGGACATGGT GGCTGTGGTG GGTGAGCAGT TTACTCTGGA ATGTGGGCCG 
CCCTGGGGCC ACCCAGAGCC CACAGTCTCA TGGTGGAAAG ATGGGAAACC CCTGGCCCTC 
CAGCCCGGAA GGCACACAGT GTCCGGGGGG TCCCTGCTGA TGGCAAGAGC AGAGAAGAGT 
GACGAAGGGA CCTACATGTG TGTGGCCACC AACAGCGCAG GACATAGGGA GAGCCGCGCA 
GCCCGGGTTT CCATCCAGGA GCCCCAGGAC TACACGGAGC CTGTGGAGCT TCTGGCTGTG 
CGAATTCAGC TGGAAAATGT GACACTGCTG AACCCGGATC CTGCAGAGGG CCCCAAGCCT 
AGACCGGCGG TGTGGCTCAG CTGGAAGGTC AGTGGCCCTG CTGCGCCTGC CCAATCTTAC 
ACGGCCTTGT TCAGGACCCA GACTGCCCCG GGAGGCCAGG GAGCTCCGTG GGCAGAGGAG 
CTGCTGGCCG GCTGGCAGAG CGCAGAGCTT GGAGGCCTCC ACTGGGGCCA AGACTACGAG 
TTCAAAGTGA GACCATCCTC TGGCCGGGCT CGAGGCCCTG ACAGCAACGT GCTGCTCCTG 
AGGCTGCCGG AAAAAGTGCC CAGTGCCCCA CCTCAGGAAG TGACTCTAAA GCCTGGCAAT 
GGCACTGTCT TTGTGAGCTG GGTCCCACCA CCTGCTGAAA ACCACAATGG CATCATCCGT 
GGCTACCAGG TCTGGAGCCT GGGCAACACA TCACTGCCAC CAGCCAACTG GACTGTAGTT 
GGTGAGCAGA CCCAGCTGGA AATCGCCACC CATATGCCAG GCTCCTACTG CGTGCAAGTG 
GCTGCAGTCA CTGGTGCTGG AGCTGGGGAG CCCAGTAGAC CTGTCTGCCT CCTTTTAGAG 
CAGGCCATGG AGCGAGCCAC CCAAGAACCC AGTGAGCATG GTCCCTGGAC CCTGGAGCAG 
CTGAGGGCTA CCTTGAAGCG GCCTGAGGTC ATTGCCACCT GCGGTGTTGC ACTCTGGCTG 
CTGCTTCTGG GCACCGCCGT GTGTATCCAC CGCCGGCGCC GAGCTAGGGT GCACCTGGGC 
CCAGGTCTGT ACAGATATAC CAGTGAGGAT GCCATCCTAA AACACAGGAT GGATCACAGT 
GACTCCCAGT GGTTGGCAGA CACTTGGCGT TCCACCTCTG GCTCTCGGGA CCTGAGCAGC 
AGCAGCAGCC TCAGCAGTCG GCTGGGGGCG GATGCCCGGG ACCCACTAGA CTGTCGTCGC 
TCCTTGCTCT CCTGGGACTC CCGAAGCCCC GGCGTGCCCC TGCTTCCAGA CACCAGCACT 
TTTTATGGCT CCCTCATCGC TGAGCTGCCC TCCAGTACCC CAGCCAGGCC AAGTCCCCAG 
GTCCCAGCTG TCAGGCGCCT CCCACCCCAG CTGGCCCAGC TCTCCAGCCC CTGTTCCAGC 
TCAGACAGCC TCTGCAGCCG CAGGGGACTC TCTTCTCCCC GCTTGTCTCT GGCCCCTGCA 
GAGGCTTGGA AGGCCAAAAA GAAGCAGGAG CTGCAGCATG CCAACAGTTC CCCACTGCTC 
CGGGGCAGCC ACTCCTTGGA GCTCCGGGCC TGTGAGTTAG GAAATAGAGG TTCCAAGAAC 
CTTTCCCAAA GCCCAGGAGC TGTGCCCCAA GCTCTGGTTG CCTGGCGGGC CCTGGGACCG 
AAACTCCTCA GCTCCTCAAA TGAGCTGGTT ACTCGTCATC TCCCTCCAGC ACCCCTCTTT 
CCTCATGAAA CTCCCCCAAC TCAGAGTCAA CAGACCCAGC CTCCGGTGGC ACCACAGGCT 
CCCTCCTCCA TCCTGCTGCC AGCAGCCCCC ATCCCCATCC TTAGCCCCTG CAGTCCCCCT 
AGCCCCCAGG CCTCTTCCCT CTCTGGCCCC AGCCCAGCTT CCAGTCGCCT GTCCAGCTCC 
TCACTGTCAT CCCTGGGGGA GGATCAAGAC AGCGTGCTGA CCCCTGAGGA GGTAGCCCTG 
TGCTTGGAAC TCAGTGAGGG TGAGGAGACT CCCAGGAACA GCGTCTCTCC CATGCCAAGG 
GCTCCTTCAC CCCCCACCAC CTATGGGTAC ATCAGCGTCC CAACAGCCTC AGAGTTCACG 
GACATGGGCA GGACTGGAGG AGGGGTGGGG CCCAAGGGGG GAGTCTTGCT GTGCCCACCT 
CGGCCCTGCC TCACCCCCAC CCCCAGCGAG GGCTCCTTAG CCAATGGTTG GGGCTCAGCC 
TCTGAGGACA ATGCCGCCAG CGCCAGAGCC AGCCTTGTCA GCTCCTCCGA TGGCTCCTTC 
CTCGCTGATG CTCACTTTGC CCGGGCCCTG GCAGTGGCTG TGGATAGCTT TGGTTTCGGT 
CTAGAGCCCA GGGAGGCAGA CTGCGTCTTC ATAGATGCCT CATCACCTCC CTCCCCACGG 
GATGAGATCT TCCTGACCCC CAACCTCTCC CTGCCCCTGT GGGAGTGGAG GCCAGACTGG 
TTGGAAGACA TGGAGGTCAG CCACACCCAG CGGCTGGGAA GGGGGATGCC TCCCTGGCCC 
CCTGACTCTC AGATCTCTTC CCAGAGAAGT CAGCTCCACT GTCGTATGCC CAAGGCTGGT 
GCTTCTCCTG TAGATTACTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO4 ( NM_019055.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_061928.4 Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant 1, mRNA.

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CloneID	OHu09210
Clone ID Related Accession (Same CDS sequence)	NM_019055.6 , NM_019055.5
Accession Version	NM_019055.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3024bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	roundabout homolog 4 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC424813.1 and AF361473.2. On Dec 3, 2008 this sequence version replaced NM_019055.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC039602.1, AK027852.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTCTG GAGGAGACAG CCTCCTGGGG GGCAGGGGTT CCCTGCCTCT GCTGCTCCTG 
CTCATCATGG GAGGCATGGC TCAGGACTCC CCGCCCCAGA TCCTAGTCCA CCCCCAGGAC 
CAGCTGTTCC AGGGCCCTGG CCCTGCCAGG ATGAGCTGCC AAGCCTCAGG CCAGCCACCT 
CCCACCATCC GCTGGTTGCT GAATGGGCAG CCCCTGAGCA TGGTGCCCCC AGACCCACAC 
CACCTCCTGC CTGATGGGAC CCTTCTGCTG CTACAGCCCC CTGCCCGGGG ACATGCCCAC 
GATGGCCAGG CCCTGTCCAC AGACCTGGGT GTCTACACAT GTGAGGCCAG CAACCGGCTT 
GGCACGGCAG TCAGCAGAGG CGCTCGGCTG TCTGTGGCTG TCCTCCGGGA GGATTTCCAG 
ATCCAGCCTC GGGACATGGT GGCTGTGGTG GGTGAGCAGT TTACTCTGGA ATGTGGGCCG 
CCCTGGGGCC ACCCAGAGCC CACAGTCTCA TGGTGGAAAG ATGGGAAACC CCTGGCCCTC 
CAGCCCGGAA GGCACACAGT GTCCGGGGGG TCCCTGCTGA TGGCAAGAGC AGAGAAGAGT 
GACGAAGGGA CCTACATGTG TGTGGCCACC AACAGCGCAG GACATAGGGA GAGCCGCGCA 
GCCCGGGTTT CCATCCAGGA GCCCCAGGAC TACACGGAGC CTGTGGAGCT TCTGGCTGTG 
CGAATTCAGC TGGAAAATGT GACACTGCTG AACCCGGATC CTGCAGAGGG CCCCAAGCCT 
AGACCGGCGG TGTGGCTCAG CTGGAAGGTC AGTGGCCCTG CTGCGCCTGC CCAATCTTAC 
ACGGCCTTGT TCAGGACCCA GACTGCCCCG GGAGGCCAGG GAGCTCCGTG GGCAGAGGAG 
CTGCTGGCCG GCTGGCAGAG CGCAGAGCTT GGAGGCCTCC ACTGGGGCCA AGACTACGAG 
TTCAAAGTGA GACCATCCTC TGGCCGGGCT CGAGGCCCTG ACAGCAACGT GCTGCTCCTG 
AGGCTGCCGG AAAAAGTGCC CAGTGCCCCA CCTCAGGAAG TGACTCTAAA GCCTGGCAAT 
GGCACTGTCT TTGTGAGCTG GGTCCCACCA CCTGCTGAAA ACCACAATGG CATCATCCGT 
GGCTACCAGG TCTGGAGCCT GGGCAACACA TCACTGCCAC CAGCCAACTG GACTGTAGTT 
GGTGAGCAGA CCCAGCTGGA AATCGCCACC CATATGCCAG GCTCCTACTG CGTGCAAGTG 
GCTGCAGTCA CTGGTGCTGG AGCTGGGGAG CCCAGTAGAC CTGTCTGCCT CCTTTTAGAG 
CAGGCCATGG AGCGAGCCAC CCAAGAACCC AGTGAGCATG GTCCCTGGAC CCTGGAGCAG 
CTGAGGGCTA CCTTGAAGCG GCCTGAGGTC ATTGCCACCT GCGGTGTTGC ACTCTGGCTG 
CTGCTTCTGG GCACCGCCGT GTGTATCCAC CGCCGGCGCC GAGCTAGGGT GCACCTGGGC 
CCAGGTCTGT ACAGATATAC CAGTGAGGAT GCCATCCTAA AACACAGGAT GGATCACAGT 
GACTCCCAGT GGTTGGCAGA CACTTGGCGT TCCACCTCTG GCTCTCGGGA CCTGAGCAGC 
AGCAGCAGCC TCAGCAGTCG GCTGGGGGCG GATGCCCGGG ACCCACTAGA CTGTCGTCGC 
TCCTTGCTCT CCTGGGACTC CCGAAGCCCC GGCGTGCCCC TGCTTCCAGA CACCAGCACT 
TTTTATGGCT CCCTCATCGC TGAGCTGCCC TCCAGTACCC CAGCCAGGCC AAGTCCCCAG 
GTCCCAGCTG TCAGGCGCCT CCCACCCCAG CTGGCCCAGC TCTCCAGCCC CTGTTCCAGC 
TCAGACAGCC TCTGCAGCCG CAGGGGACTC TCTTCTCCCC GCTTGTCTCT GGCCCCTGCA 
GAGGCTTGGA AGGCCAAAAA GAAGCAGGAG CTGCAGCATG CCAACAGTTC CCCACTGCTC 
CGGGGCAGCC ACTCCTTGGA GCTCCGGGCC TGTGAGTTAG GAAATAGAGG TTCCAAGAAC 
CTTTCCCAAA GCCCAGGAGC TGTGCCCCAA GCTCTGGTTG CCTGGCGGGC CCTGGGACCG 
AAACTCCTCA GCTCCTCAAA TGAGCTGGTT ACTCGTCATC TCCCTCCAGC ACCCCTCTTT 
CCTCATGAAA CTCCCCCAAC TCAGAGTCAA CAGACCCAGC CTCCGGTGGC ACCACAGGCT 
CCCTCCTCCA TCCTGCTGCC AGCAGCCCCC ATCCCCATCC TTAGCCCCTG CAGTCCCCCT 
AGCCCCCAGG CCTCTTCCCT CTCTGGCCCC AGCCCAGCTT CCAGTCGCCT GTCCAGCTCC 
TCACTGTCAT CCCTGGGGGA GGATCAAGAC AGCGTGCTGA CCCCTGAGGA GGTAGCCCTG 
TGCTTGGAAC TCAGTGAGGG TGAGGAGACT CCCAGGAACA GCGTCTCTCC CATGCCAAGG 
GCTCCTTCAC CCCCCACCAC CTATGGGTAC ATCAGCGTCC CAACAGCCTC AGAGTTCACG 
GACATGGGCA GGACTGGAGG AGGGGTGGGG CCCAAGGGGG GAGTCTTGCT GTGCCCACCT 
CGGCCCTGCC TCACCCCCAC CCCCAGCGAG GGCTCCTTAG CCAATGGTTG GGGCTCAGCC 
TCTGAGGACA ATGCCGCCAG CGCCAGAGCC AGCCTTGTCA GCTCCTCCGA TGGCTCCTTC 
CTCGCTGATG CTCACTTTGC CCGGGCCCTG GCAGTGGCTG TGGATAGCTT TGGTTTCGGT 
CTAGAGCCCA GGGAGGCAGA CTGCGTCTTC ATAGATGCCT CATCACCTCC CTCCCCACGG 
GATGAGATCT TCCTGACCCC CAACCTCTCC CTGCCCCTGT GGGAGTGGAG GCCAGACTGG 
TTGGAAGACA TGGAGGTCAG CCACACCCAG CGGCTGGGAA GGGGGATGCC TCCCTGGCCC 
CCTGACTCTC AGATCTCTTC CCAGAGAAGT CAGCTCCACT GTCGTATGCC CAAGGCTGGT 
GCTTCTCCTG TAGATTACTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061928.4
CDS	141..3164
Translation	MGSGGDSLLGGRGSLPLLLLLIMGGMAQDSPPQILVHPQDQLFQGPGPARMSCQASGQPPPTIRWLLNGQPLSMVPPDPHHLLPDGTLLLLQPPARGHAHDGQALSTDLGVYTCEASNRLGTAVSRGARLSVAVLREDFQIQPRDMVAVVGEQFTLECGPPWGHPEPTVSWWKDGKPLALQPGRHTVSGGSLLMARAEKSDEGTYMCVATNSAGHRESRAARVSIQEPQDYTEPVELLAVRIQLENVTLLNPDPAEGPKPRPAVWLSWKVSGPAAPAQSYTALFRTQTAPGGQGAPWAEELLAGWQSAELGGLHWGQDYEFKVRPSSGRARGPDSNVLLLRLPEKVPSAPPQEVTLKPGNGTVFVSWVPPPAENHNGIIRGYQVWSLGNTSLPPANWTVVGEQTQLEIATHMPGSYCVQVAAVTGAGAGEPSRPVCLLLEQAMERATQEPSEHGPWTLEQLRATLKRPEVIATCGVALWLLLLGTAVCIHRRRRARVHLGPGLYRYTSEDAILKHRMDHSDSQWLADTWRSTSGSRDLSSSSSLSSRLGADARDPLDCRRSLLSWDSRSPGVPLLPDTSTFYGSLIAELPSSTPARPSPQVPAVRRLPPQLAQLSSPCSSSDSLCSRRGLSSPRLSLAPAEAWKAKKKQELQHANSSPLLRGSHSLELRACELGNRGSKNLSQSPGAVPQALVAWRALGPKLLSSSNELVTRHLPPAPLFPHETPPTQSQQTQPPVAPQAPSSILLPAAPIPILSPCSPPSPQASSLSGPSPASSRLSSSSLSSLGEDQDSVLTPEEVALCLELSEGEETPRNSVSPMPRAPSPPTTYGYISVPTASEFTDMGRTGGGVGPKGGVLLCPPRPCLTPTPSEGSLANGWGSASEDNAASARASLVSSSDGSFLADAHFARALAVAVDSFGFGLEPREADCVFIDASSPPSPRDEIFLTPNLSLPLWEWRPDWLEDMEVSHTQRLGRGMPPWPPDSQISSQRSQLHCRMPKAGASPVDYS

Target ORF information:

RefSeq Version	NM_019055.5
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 4 (ROBO4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019055.5

ORF Insert Sequence:

ATGGGCTCTG GAGGAGACAG CCTCCTGGGG GGCAGGGGTT CCCTGCCTCT GCTGCTCCTG 
CTCATCATGG GAGGCATGGC TCAGGACTCC CCGCCCCAGA TCCTAGTCCA CCCCCAGGAC 
CAGCTGTTCC AGGGCCCTGG CCCTGCCAGG ATGAGCTGCC AAGCCTCAGG CCAGCCACCT 
CCCACCATCC GCTGGTTGCT GAATGGGCAG CCCCTGAGCA TGGTGCCCCC AGACCCACAC 
CACCTCCTGC CTGATGGGAC CCTTCTGCTG CTACAGCCCC CTGCCCGGGG ACATGCCCAC 
GATGGCCAGG CCCTGTCCAC AGACCTGGGT GTCTACACAT GTGAGGCCAG CAACCGGCTT 
GGCACGGCAG TCAGCAGAGG CGCTCGGCTG TCTGTGGCTG TCCTCCGGGA GGATTTCCAG 
ATCCAGCCTC GGGACATGGT GGCTGTGGTG GGTGAGCAGT TTACTCTGGA ATGTGGGCCG 
CCCTGGGGCC ACCCAGAGCC CACAGTCTCA TGGTGGAAAG ATGGGAAACC CCTGGCCCTC 
CAGCCCGGAA GGCACACAGT GTCCGGGGGG TCCCTGCTGA TGGCAAGAGC AGAGAAGAGT 
GACGAAGGGA CCTACATGTG TGTGGCCACC AACAGCGCAG GACATAGGGA GAGCCGCGCA 
GCCCGGGTTT CCATCCAGGA GCCCCAGGAC TACACGGAGC CTGTGGAGCT TCTGGCTGTG 
CGAATTCAGC TGGAAAATGT GACACTGCTG AACCCGGATC CTGCAGAGGG CCCCAAGCCT 
AGACCGGCGG TGTGGCTCAG CTGGAAGGTC AGTGGCCCTG CTGCGCCTGC CCAATCTTAC 
ACGGCCTTGT TCAGGACCCA GACTGCCCCG GGAGGCCAGG GAGCTCCGTG GGCAGAGGAG 
CTGCTGGCCG GCTGGCAGAG CGCAGAGCTT GGAGGCCTCC ACTGGGGCCA AGACTACGAG 
TTCAAAGTGA GACCATCCTC TGGCCGGGCT CGAGGCCCTG ACAGCAACGT GCTGCTCCTG 
AGGCTGCCGG AAAAAGTGCC CAGTGCCCCA CCTCAGGAAG TGACTCTAAA GCCTGGCAAT 
GGCACTGTCT TTGTGAGCTG GGTCCCACCA CCTGCTGAAA ACCACAATGG CATCATCCGT 
GGCTACCAGG TCTGGAGCCT GGGCAACACA TCACTGCCAC CAGCCAACTG GACTGTAGTT 
GGTGAGCAGA CCCAGCTGGA AATCGCCACC CATATGCCAG GCTCCTACTG CGTGCAAGTG 
GCTGCAGTCA CTGGTGCTGG AGCTGGGGAG CCCAGTAGAC CTGTCTGCCT CCTTTTAGAG 
CAGGCCATGG AGCGAGCCAC CCAAGAACCC AGTGAGCATG GTCCCTGGAC CCTGGAGCAG 
CTGAGGGCTA CCTTGAAGCG GCCTGAGGTC ATTGCCACCT GCGGTGTTGC ACTCTGGCTG 
CTGCTTCTGG GCACCGCCGT GTGTATCCAC CGCCGGCGCC GAGCTAGGGT GCACCTGGGC 
CCAGGTCTGT ACAGATATAC CAGTGAGGAT GCCATCCTAA AACACAGGAT GGATCACAGT 
GACTCCCAGT GGTTGGCAGA CACTTGGCGT TCCACCTCTG GCTCTCGGGA CCTGAGCAGC 
AGCAGCAGCC TCAGCAGTCG GCTGGGGGCG GATGCCCGGG ACCCACTAGA CTGTCGTCGC 
TCCTTGCTCT CCTGGGACTC CCGAAGCCCC GGCGTGCCCC TGCTTCCAGA CACCAGCACT 
TTTTATGGCT CCCTCATCGC TGAGCTGCCC TCCAGTACCC CAGCCAGGCC AAGTCCCCAG 
GTCCCAGCTG TCAGGCGCCT CCCACCCCAG CTGGCCCAGC TCTCCAGCCC CTGTTCCAGC 
TCAGACAGCC TCTGCAGCCG CAGGGGACTC TCTTCTCCCC GCTTGTCTCT GGCCCCTGCA 
GAGGCTTGGA AGGCCAAAAA GAAGCAGGAG CTGCAGCATG CCAACAGTTC CCCACTGCTC 
CGGGGCAGCC ACTCCTTGGA GCTCCGGGCC TGTGAGTTAG GAAATAGAGG TTCCAAGAAC 
CTTTCCCAAA GCCCAGGAGC TGTGCCCCAA GCTCTGGTTG CCTGGCGGGC CCTGGGACCG 
AAACTCCTCA GCTCCTCAAA TGAGCTGGTT ACTCGTCATC TCCCTCCAGC ACCCCTCTTT 
CCTCATGAAA CTCCCCCAAC TCAGAGTCAA CAGACCCAGC CTCCGGTGGC ACCACAGGCT 
CCCTCCTCCA TCCTGCTGCC AGCAGCCCCC ATCCCCATCC TTAGCCCCTG CAGTCCCCCT 
AGCCCCCAGG CCTCTTCCCT CTCTGGCCCC AGCCCAGCTT CCAGTCGCCT GTCCAGCTCC 
TCACTGTCAT CCCTGGGGGA GGATCAAGAC AGCGTGCTGA CCCCTGAGGA GGTAGCCCTG 
TGCTTGGAAC TCAGTGAGGG TGAGGAGACT CCCAGGAACA GCGTCTCTCC CATGCCAAGG 
GCTCCTTCAC CCCCCACCAC CTATGGGTAC ATCAGCGTCC CAACAGCCTC AGAGTTCACG 
GACATGGGCA GGACTGGAGG AGGGGTGGGG CCCAAGGGGG GAGTCTTGCT GTGCCCACCT 
CGGCCCTGCC TCACCCCCAC CCCCAGCGAG GGCTCCTTAG CCAATGGTTG GGGCTCAGCC 
TCTGAGGACA ATGCCGCCAG CGCCAGAGCC AGCCTTGTCA GCTCCTCCGA TGGCTCCTTC 
CTCGCTGATG CTCACTTTGC CCGGGCCCTG GCAGTGGCTG TGGATAGCTT TGGTTTCGGT 
CTAGAGCCCA GGGAGGCAGA CTGCGTCTTC ATAGATGCCT CATCACCTCC CTCCCCACGG 
GATGAGATCT TCCTGACCCC CAACCTCTCC CTGCCCCTGT GGGAGTGGAG GCCAGACTGG 
TTGGAAGACA TGGAGGTCAG CCACACCCAG CGGCTGGGAA GGGGGATGCC TCCCTGGCCC 
CCTGACTCTC AGATCTCTTC CCAGAGAAGT CAGCTCCACT GTCGTATGCC CAAGGCTGGT 
GCTTCTCCTG TAGATTACTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Endothelial Robo4 regulates IL-6 production by endothelial cells and monocytes via a crosstalk mechanism in inflammation.
Biochemical and biophysical research communications495(1)801-806(2018 01)
Shirakura K,Ishiba R,Kashio T,Sakai M,Fukushima Y,Yamamoto N,Manabe S,Shigesada N,Tanaka T,Hino N,Aird WC,Doi T,Okada Y

Transcription factor SP1 mediates hyperglycemia-induced upregulation of roundabout4 in retinal microvascular endothelial cells.
Gene61631-40(2017 Jun)
Xie J,Gong Q,Liu X,Liu Z,Tian R,Cheng Y,Su G

Validation of RT-qPCR reference genes and determination of Robo4 expression levels in human retinal endothelial cells under hypoxia and/or hyperglycemia.
Gene585(1)135-142(2016 Jul)
Xie J,Liu X,Li Y,Liu Y,Su G

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ROBO4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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