ROBO4 cDNA ORF clone, Canis lupus familiaris(dog)

The following ROBO4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROBO4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe05768 XM_005619614.3
Latest version!
Canis lupus familiaris roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe05769 XM_546424.6
Latest version!
Canis lupus familiaris roundabout guidance receptor 4 (ROBO4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe05770 XM_005619615.3
Latest version!
Canis lupus familiaris roundabout guidance receptor 4 (ROBO4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe287891 XM_022418150.1
Latest version!
Canis lupus familiaris roundabout guidance receptor 4 (ROBO4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
View Old Accession Versions >>
OCe05769 XM_546424.5 Canis lupus familiaris roundabout guidance receptor 4 (ROBO4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe05768 XM_005619614.2 Canis lupus familiaris roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe05770 XM_005619615.2 Canis lupus familiaris roundabout guidance receptor 4 (ROBO4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe05768
    Clone ID Related Accession (Same CDS sequence) XM_005619614.2 , XM_005619614.3
    Accession Version XM_005619614.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619614.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGCTCTG GAGGAGCAGG CCTCCTTGGG CCCCTGGGGC CCCTGCCTCT CCTGCTTCTG 
    CTTGTCACGG GAGGCACGGC TCAGGACTCC CCACCCCAGA TCCTAGTCCA CCCCCAGGAC
    CAGCTGCTCC AGGGCCCTGG CCCCGCCAAA ATGAGCTGCC GAGCCTCGGG CCAGCCACCC
    CCCACCATCC GCTGGCTGCT GAATGGGCAA CCCCTGAGCA TGGTGCCCCC AGACGTCCAC
    CACCTCCTTC CTGATGGGAC GCTCCTGCTG CTGCGGCCCC CTGCCCGGGG ACGTGCCCAC
    GACGACCAGG CCCCATCCAC AGACCTGGGC ATCTACACGT GTGAGGCCAG CAACAGGCTG
    GGCACAGCAG TCAGCCGAGG AGCTCGGCTG TCTGTGGCTG TCCTTCGGGA GGACTTCCAG
    ACCCAGCCTC AGGATACAGT GGCCACTGTG GGCGAGCAGG TGATTCTGGA GTGTGGGCCG
    CCCTTGGGCC ATCCAGAGCC CACAGTCTCG TGGTGGAAAG ATGGCAAACC CCTGGCCCTA
    CGGCCAGGGC GGCACTCGGT CTCCAGGGGC TCCCTGCAGA TGGCAAGAGC AGAGAAGAGC
    GATGCAGGGA CCTATATGTG CGTGGCCACC AACAGCGCAG GACAACGGGA GAGCCGGGCA
    GCCAGGGTAT CTGTCCAGGA GCCCCAGGAC TACAAGAAGC CTCTGGAGCT GCTGGCCGTG
    CACATTCAGC TGGAAAATGT GACCTTGCTG AACCCAGACC CCGCAAAGAG CGCCAAGCCT
    GAGCCTGCTG TGTGGCTCAG CTGGAAGGTG AGCGGCCCTG CAGCACCTGC TCAGTCCTAC
    ACAGCCCTCT TCAGGGTCCA GACGGCCTCA GGAGGCCCGG GAGCTCCATG GGCAGAGGCC
    CTGCTGGCTG GCTGGCAAAG CGCGGAGCTT GGAGGCCTCC TTTGGGGCCA AGACTACGAG
    TTCAAAGTGA GACCATCCTC TGGCCGGGCT CAAGGCCCTG ACAGCAACGT GCTGCTCCTG
    AGGCTGCCCG AGCAAGTGCC CAGTGCCCCT CCCCAGGAGG TGGCCTTAAA ACCTGGCAAT
    GGCAGTGTCC TTGTAAGCTG GGTCCCACCA CCTGCTGAAA ACCACAATGG CATCATCCGT
    GGCTACCAGA TCTGGAGCCT GGGCAACACC TCGCTGCCCC CCGCCAACTG GACCGTGGCC
    GGTGAGCAGA CCCAGCTGGA GATCGCCACC CGAATGCCAG GTTCCTACTG TGTACAAGTG
    GCTGCAGTCA CTGGCGCCGG GACTGGGCCT CCCAGTAGCC CTGTCTGCCT CCTTTTAGAG
    CGGACCATGG AGCGAGCCAC CCAAGAGCTC AGTGATGGTC CCTGGACTCT GGAGCAGCTG
    AGGGCCACCT TGAGGCGGCC AGAAGTGATT GCTGGTGGGG GTGTCCTGCT CTGGCTGGTG
    CTGCTGGGCA CCGCCGTGTG TGTACACCGC AGGCGCCGAG CTGGGGTGCA CCTGGGCCCA
    GGTTTGTACA GGTACACCAG TGAGGATGCC ATCCTAAAGC ACAGGATGGA TCATAGTGAC
    TCCCCTTGGC TGGCAGACAC CTGGCGTTCC ACCTCCGGCT CTCGGGACCT GAGCAGCAGC
    AGCAGCCTCA ACAGCAGGCT GGGAGTGGAC CCCCGGGACC CACTAGACTG TCGCCGCTCC
    CTGATCTCCT GGGATCCCCG AAGCCCTGGT GTGCCCCTGC TTCCCGACAC CAGCACTTTT
    TATGGCTCCC TCATCGCTGA GCTGCCTGCC GGCCCCCCGG CCCGGCCAAG CCCCAAGACC
    CCAGCTGTCA GGCGTCTCCC ACCCCAGCTG GCCCGGCTCT CCAGCCCCTG GCCCAGTCCC
    GACAGCCTCG GCAGCCGCAG GGGCCTCGCT TCTCCTCTGG CCCCTGCAGA GGCTTGGAAG
    GCCAAAAAGA AGCAGGAGCT GCAACAAGCC AATAGCTCCC CGCCCCTCCG GGCCAGCCGC
    CCTATGGAGC TCTGGGCCTG GGAGTTGGGG AACAGAGGCT CCAAGAACCT TTCCCAAAGC
    CCAGGCTTTT GCTCCCCAGG AGCTGTGCCC CAGGCTCTGG TGGCCTGGAG GGCCCTGGGA
    CCGCAGCTCC TCGGCTCCTC CAATGAGATG GTTTCATGCC CTCTCCCCTC AGCACCCCTC
    GCTCCGCGTG GAGCCCCTAC TCAGAGTCAG CAGACCCAGC ACTCTGCAGA GCCCCAAGCT
    CCGTCCTCCC ACCCACTGCC AGCAGCCCCC ATCCTGATCC CCTCTAGCCC CCAGGCTTCT
    TCCCTCTCCG GGCCCAGCCC AGAGTCCAGT CGTCTGTCCA GCTCTTCACT GTCATCCCTG
    GAGGAGGATC AGGACAGCGT GCTGACTCCT GAGGAGGTGG CCCTGTGCCT GGAGCTCAGC
    GAGGGTGAGG AGACCCCCAG GAATAGTGTC TCTCCTATGC CAAGGGCTCC TTCACCCCCT
    GTCACCTACG GCTACATCAG CATCCCGGCT GCCTCAGAGC TGGCAGACAT GGGCAGGGCC
    GGAGGAGGGG TGGGATCCGA AGTGAGGGGC TTGCTGTGCC CGCCCCGGCC CTGCCCCACC
    CCTACCCCCA GCGAGGGCTC CCTGGCCAAT GGCTGGGGTT CAGCCTCCGA GGACAATGCC
    CCCAGTGCCC GAGCCAGCCT CGTCAGCTCC TCCGATGGCT CCTTCCTTGC TGATGCTCAC
    TTTGCGCGGG CCCTGGCGAT GGCTGTGGAC AGCTTCGGCT TTGGTCTGGA GCCCAGGGAC
    GTGGACTGCG TCTTCACGGA TGCCTCCTCT CCTCCTTCCC CCCGCGATGA CCTCTTCCTG
    ACCTCCACCC TCTCCCTGCC CCTGTGGGAG TGGAGGTCAG ACTGGCTGGA GGACATGGAG
    AACAACCGTA GCCAGCGGCT GGGAAGGGGG CTGCCTCCCT GGCCCCCTGA TTCCCGGCGC
    TCTTCCAGAA GGAGTCAGCT GGGCCGTCCT GAGCCCAAGG CCGCCAACTC CTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619671.1
    CDS118..3114
    Translation

    Target ORF information:

    RefSeq Version XM_005619614.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619614.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGCTCTG GAGGAGCAGG CCTCCTTGGG CCCCTGGGGC CCCTGCCTCT CCTGCTTCTG 
    CTTGTCACGG GAGGCACGGC TCAGGACTCC CCACCCCAGA TCCTAGTCCA CCCCCAGGAC
    CAGCTGCTCC AGGGCCCTGG CCCCGCCAAA ATGAGCTGCC GAGCCTCGGG CCAGCCACCC
    CCCACCATCC GCTGGCTGCT GAATGGGCAA CCCCTGAGCA TGGTGCCCCC AGACGTCCAC
    CACCTCCTTC CTGATGGGAC GCTCCTGCTG CTGCGGCCCC CTGCCCGGGG ACGTGCCCAC
    GACGACCAGG CCCCATCCAC AGACCTGGGC ATCTACACGT GTGAGGCCAG CAACAGGCTG
    GGCACAGCAG TCAGCCGAGG AGCTCGGCTG TCTGTGGCTG TCCTTCGGGA GGACTTCCAG
    ACCCAGCCTC AGGATACAGT GGCCACTGTG GGCGAGCAGG TGATTCTGGA GTGTGGGCCG
    CCCTTGGGCC ATCCAGAGCC CACAGTCTCG TGGTGGAAAG ATGGCAAACC CCTGGCCCTA
    CGGCCAGGGC GGCACTCGGT CTCCAGGGGC TCCCTGCAGA TGGCAAGAGC AGAGAAGAGC
    GATGCAGGGA CCTATATGTG CGTGGCCACC AACAGCGCAG GACAACGGGA GAGCCGGGCA
    GCCAGGGTAT CTGTCCAGGA GCCCCAGGAC TACAAGAAGC CTCTGGAGCT GCTGGCCGTG
    CACATTCAGC TGGAAAATGT GACCTTGCTG AACCCAGACC CCGCAAAGAG CGCCAAGCCT
    GAGCCTGCTG TGTGGCTCAG CTGGAAGGTG AGCGGCCCTG CAGCACCTGC TCAGTCCTAC
    ACAGCCCTCT TCAGGGTCCA GACGGCCTCA GGAGGCCCGG GAGCTCCATG GGCAGAGGCC
    CTGCTGGCTG GCTGGCAAAG CGCGGAGCTT GGAGGCCTCC TTTGGGGCCA AGACTACGAG
    TTCAAAGTGA GACCATCCTC TGGCCGGGCT CAAGGCCCTG ACAGCAACGT GCTGCTCCTG
    AGGCTGCCCG AGCAAGTGCC CAGTGCCCCT CCCCAGGAGG TGGCCTTAAA ACCTGGCAAT
    GGCAGTGTCC TTGTAAGCTG GGTCCCACCA CCTGCTGAAA ACCACAATGG CATCATCCGT
    GGCTACCAGA TCTGGAGCCT GGGCAACACC TCGCTGCCCC CCGCCAACTG GACCGTGGCC
    GGTGAGCAGA CCCAGCTGGA GATCGCCACC CGAATGCCAG GTTCCTACTG TGTACAAGTG
    GCTGCAGTCA CTGGCGCCGG GACTGGGCCT CCCAGTAGCC CTGTCTGCCT CCTTTTAGAG
    CGGACCATGG AGCGAGCCAC CCAAGAGCTC AGTGATGGTC CCTGGACTCT GGAGCAGCTG
    AGGGCCACCT TGAGGCGGCC AGAAGTGATT GCTGGTGGGG GTGTCCTGCT CTGGCTGGTG
    CTGCTGGGCA CCGCCGTGTG TGTACACCGC AGGCGCCGAG CTGGGGTGCA CCTGGGCCCA
    GGTTTGTACA GGTACACCAG TGAGGATGCC ATCCTAAAGC ACAGGATGGA TCATAGTGAC
    TCCCCTTGGC TGGCAGACAC CTGGCGTTCC ACCTCCGGCT CTCGGGACCT GAGCAGCAGC
    AGCAGCCTCA ACAGCAGGCT GGGAGTGGAC CCCCGGGACC CACTAGACTG TCGCCGCTCC
    CTGATCTCCT GGGATCCCCG AAGCCCTGGT GTGCCCCTGC TTCCCGACAC CAGCACTTTT
    TATGGCTCCC TCATCGCTGA GCTGCCTGCC GGCCCCCCGG CCCGGCCAAG CCCCAAGACC
    CCAGCTGTCA GGCGTCTCCC ACCCCAGCTG GCCCGGCTCT CCAGCCCCTG GCCCAGTCCC
    GACAGCCTCG GCAGCCGCAG GGGCCTCGCT TCTCCTCTGG CCCCTGCAGA GGCTTGGAAG
    GCCAAAAAGA AGCAGGAGCT GCAACAAGCC AATAGCTCCC CGCCCCTCCG GGCCAGCCGC
    CCTATGGAGC TCTGGGCCTG GGAGTTGGGG AACAGAGGCT CCAAGAACCT TTCCCAAAGC
    CCAGGCTTTT GCTCCCCAGG AGCTGTGCCC CAGGCTCTGG TGGCCTGGAG GGCCCTGGGA
    CCGCAGCTCC TCGGCTCCTC CAATGAGATG GTTTCATGCC CTCTCCCCTC AGCACCCCTC
    GCTCCGCGTG GAGCCCCTAC TCAGAGTCAG CAGACCCAGC ACTCTGCAGA GCCCCAAGCT
    CCGTCCTCCC ACCCACTGCC AGCAGCCCCC ATCCTGATCC CCTCTAGCCC CCAGGCTTCT
    TCCCTCTCCG GGCCCAGCCC AGAGTCCAGT CGTCTGTCCA GCTCTTCACT GTCATCCCTG
    GAGGAGGATC AGGACAGCGT GCTGACTCCT GAGGAGGTGG CCCTGTGCCT GGAGCTCAGC
    GAGGGTGAGG AGACCCCCAG GAATAGTGTC TCTCCTATGC CAAGGGCTCC TTCACCCCCT
    GTCACCTACG GCTACATCAG CATCCCGGCT GCCTCAGAGC TGGCAGACAT GGGCAGGGCC
    GGAGGAGGGG TGGGATCCGA AGTGAGGGGC TTGCTGTGCC CGCCCCGGCC CTGCCCCACC
    CCTACCCCCA GCGAGGGCTC CCTGGCCAAT GGCTGGGGTT CAGCCTCCGA GGACAATGCC
    CCCAGTGCCC GAGCCAGCCT CGTCAGCTCC TCCGATGGCT CCTTCCTTGC TGATGCTCAC
    TTTGCGCGGG CCCTGGCGAT GGCTGTGGAC AGCTTCGGCT TTGGTCTGGA GCCCAGGGAC
    GTGGACTGCG TCTTCACGGA TGCCTCCTCT CCTCCTTCCC CCCGCGATGA CCTCTTCCTG
    ACCTCCACCC TCTCCCTGCC CCTGTGGGAG TGGAGGTCAG ACTGGCTGGA GGACATGGAG
    AACAACCGTA GCCAGCGGCT GGGAAGGGGG CTGCCTCCCT GGCCCCCTGA TTCCCGGCGC
    TCTTCCAGAA GGAGTCAGCT GGGCCGTCCT GAGCCCAAGG CCGCCAACTC CTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05768
    Clone ID Related Accession (Same CDS sequence) XM_005619614.2 , XM_005619614.3
    Accession Version XM_005619614.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619614.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGCTCTG GAGGAGCAGG CCTCCTTGGG CCCCTGGGGC CCCTGCCTCT CCTGCTTCTG 
    CTTGTCACGG GAGGCACGGC TCAGGACTCC CCACCCCAGA TCCTAGTCCA CCCCCAGGAC
    CAGCTGCTCC AGGGCCCTGG CCCCGCCAAA ATGAGCTGCC GAGCCTCGGG CCAGCCACCC
    CCCACCATCC GCTGGCTGCT GAATGGGCAA CCCCTGAGCA TGGTGCCCCC AGACGTCCAC
    CACCTCCTTC CTGATGGGAC GCTCCTGCTG CTGCGGCCCC CTGCCCGGGG ACGTGCCCAC
    GACGACCAGG CCCCATCCAC AGACCTGGGC ATCTACACGT GTGAGGCCAG CAACAGGCTG
    GGCACAGCAG TCAGCCGAGG AGCTCGGCTG TCTGTGGCTG TCCTTCGGGA GGACTTCCAG
    ACCCAGCCTC AGGATACAGT GGCCACTGTG GGCGAGCAGG TGATTCTGGA GTGTGGGCCG
    CCCTTGGGCC ATCCAGAGCC CACAGTCTCG TGGTGGAAAG ATGGCAAACC CCTGGCCCTA
    CGGCCAGGGC GGCACTCGGT CTCCAGGGGC TCCCTGCAGA TGGCAAGAGC AGAGAAGAGC
    GATGCAGGGA CCTATATGTG CGTGGCCACC AACAGCGCAG GACAACGGGA GAGCCGGGCA
    GCCAGGGTAT CTGTCCAGGA GCCCCAGGAC TACAAGAAGC CTCTGGAGCT GCTGGCCGTG
    CACATTCAGC TGGAAAATGT GACCTTGCTG AACCCAGACC CCGCAAAGAG CGCCAAGCCT
    GAGCCTGCTG TGTGGCTCAG CTGGAAGGTG AGCGGCCCTG CAGCACCTGC TCAGTCCTAC
    ACAGCCCTCT TCAGGGTCCA GACGGCCTCA GGAGGCCCGG GAGCTCCATG GGCAGAGGCC
    CTGCTGGCTG GCTGGCAAAG CGCGGAGCTT GGAGGCCTCC TTTGGGGCCA AGACTACGAG
    TTCAAAGTGA GACCATCCTC TGGCCGGGCT CAAGGCCCTG ACAGCAACGT GCTGCTCCTG
    AGGCTGCCCG AGCAAGTGCC CAGTGCCCCT CCCCAGGAGG TGGCCTTAAA ACCTGGCAAT
    GGCAGTGTCC TTGTAAGCTG GGTCCCACCA CCTGCTGAAA ACCACAATGG CATCATCCGT
    GGCTACCAGA TCTGGAGCCT GGGCAACACC TCGCTGCCCC CCGCCAACTG GACCGTGGCC
    GGTGAGCAGA CCCAGCTGGA GATCGCCACC CGAATGCCAG GTTCCTACTG TGTACAAGTG
    GCTGCAGTCA CTGGCGCCGG GACTGGGCCT CCCAGTAGCC CTGTCTGCCT CCTTTTAGAG
    CGGACCATGG AGCGAGCCAC CCAAGAGCTC AGTGATGGTC CCTGGACTCT GGAGCAGCTG
    AGGGCCACCT TGAGGCGGCC AGAAGTGATT GCTGGTGGGG GTGTCCTGCT CTGGCTGGTG
    CTGCTGGGCA CCGCCGTGTG TGTACACCGC AGGCGCCGAG CTGGGGTGCA CCTGGGCCCA
    GGTTTGTACA GGTACACCAG TGAGGATGCC ATCCTAAAGC ACAGGATGGA TCATAGTGAC
    TCCCCTTGGC TGGCAGACAC CTGGCGTTCC ACCTCCGGCT CTCGGGACCT GAGCAGCAGC
    AGCAGCCTCA ACAGCAGGCT GGGAGTGGAC CCCCGGGACC CACTAGACTG TCGCCGCTCC
    CTGATCTCCT GGGATCCCCG AAGCCCTGGT GTGCCCCTGC TTCCCGACAC CAGCACTTTT
    TATGGCTCCC TCATCGCTGA GCTGCCTGCC GGCCCCCCGG CCCGGCCAAG CCCCAAGACC
    CCAGCTGTCA GGCGTCTCCC ACCCCAGCTG GCCCGGCTCT CCAGCCCCTG GCCCAGTCCC
    GACAGCCTCG GCAGCCGCAG GGGCCTCGCT TCTCCTCTGG CCCCTGCAGA GGCTTGGAAG
    GCCAAAAAGA AGCAGGAGCT GCAACAAGCC AATAGCTCCC CGCCCCTCCG GGCCAGCCGC
    CCTATGGAGC TCTGGGCCTG GGAGTTGGGG AACAGAGGCT CCAAGAACCT TTCCCAAAGC
    CCAGGCTTTT GCTCCCCAGG AGCTGTGCCC CAGGCTCTGG TGGCCTGGAG GGCCCTGGGA
    CCGCAGCTCC TCGGCTCCTC CAATGAGATG GTTTCATGCC CTCTCCCCTC AGCACCCCTC
    GCTCCGCGTG GAGCCCCTAC TCAGAGTCAG CAGACCCAGC ACTCTGCAGA GCCCCAAGCT
    CCGTCCTCCC ACCCACTGCC AGCAGCCCCC ATCCTGATCC CCTCTAGCCC CCAGGCTTCT
    TCCCTCTCCG GGCCCAGCCC AGAGTCCAGT CGTCTGTCCA GCTCTTCACT GTCATCCCTG
    GAGGAGGATC AGGACAGCGT GCTGACTCCT GAGGAGGTGG CCCTGTGCCT GGAGCTCAGC
    GAGGGTGAGG AGACCCCCAG GAATAGTGTC TCTCCTATGC CAAGGGCTCC TTCACCCCCT
    GTCACCTACG GCTACATCAG CATCCCGGCT GCCTCAGAGC TGGCAGACAT GGGCAGGGCC
    GGAGGAGGGG TGGGATCCGA AGTGAGGGGC TTGCTGTGCC CGCCCCGGCC CTGCCCCACC
    CCTACCCCCA GCGAGGGCTC CCTGGCCAAT GGCTGGGGTT CAGCCTCCGA GGACAATGCC
    CCCAGTGCCC GAGCCAGCCT CGTCAGCTCC TCCGATGGCT CCTTCCTTGC TGATGCTCAC
    TTTGCGCGGG CCCTGGCGAT GGCTGTGGAC AGCTTCGGCT TTGGTCTGGA GCCCAGGGAC
    GTGGACTGCG TCTTCACGGA TGCCTCCTCT CCTCCTTCCC CCCGCGATGA CCTCTTCCTG
    ACCTCCACCC TCTCCCTGCC CCTGTGGGAG TGGAGGTCAG ACTGGCTGGA GGACATGGAG
    AACAACCGTA GCCAGCGGCT GGGAAGGGGG CTGCCTCCCT GGCCCCCTGA TTCCCGGCGC
    TCTTCCAGAA GGAGTCAGCT GGGCCGTCCT GAGCCCAAGG CCGCCAACTC CTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619671.1
    CDS126..3122
    Translation

    Target ORF information:

    RefSeq Version XM_005619614.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 4 (ROBO4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619614.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGCTCTG GAGGAGCAGG CCTCCTTGGG CCCCTGGGGC CCCTGCCTCT CCTGCTTCTG 
    CTTGTCACGG GAGGCACGGC TCAGGACTCC CCACCCCAGA TCCTAGTCCA CCCCCAGGAC
    CAGCTGCTCC AGGGCCCTGG CCCCGCCAAA ATGAGCTGCC GAGCCTCGGG CCAGCCACCC
    CCCACCATCC GCTGGCTGCT GAATGGGCAA CCCCTGAGCA TGGTGCCCCC AGACGTCCAC
    CACCTCCTTC CTGATGGGAC GCTCCTGCTG CTGCGGCCCC CTGCCCGGGG ACGTGCCCAC
    GACGACCAGG CCCCATCCAC AGACCTGGGC ATCTACACGT GTGAGGCCAG CAACAGGCTG
    GGCACAGCAG TCAGCCGAGG AGCTCGGCTG TCTGTGGCTG TCCTTCGGGA GGACTTCCAG
    ACCCAGCCTC AGGATACAGT GGCCACTGTG GGCGAGCAGG TGATTCTGGA GTGTGGGCCG
    CCCTTGGGCC ATCCAGAGCC CACAGTCTCG TGGTGGAAAG ATGGCAAACC CCTGGCCCTA
    CGGCCAGGGC GGCACTCGGT CTCCAGGGGC TCCCTGCAGA TGGCAAGAGC AGAGAAGAGC
    GATGCAGGGA CCTATATGTG CGTGGCCACC AACAGCGCAG GACAACGGGA GAGCCGGGCA
    GCCAGGGTAT CTGTCCAGGA GCCCCAGGAC TACAAGAAGC CTCTGGAGCT GCTGGCCGTG
    CACATTCAGC TGGAAAATGT GACCTTGCTG AACCCAGACC CCGCAAAGAG CGCCAAGCCT
    GAGCCTGCTG TGTGGCTCAG CTGGAAGGTG AGCGGCCCTG CAGCACCTGC TCAGTCCTAC
    ACAGCCCTCT TCAGGGTCCA GACGGCCTCA GGAGGCCCGG GAGCTCCATG GGCAGAGGCC
    CTGCTGGCTG GCTGGCAAAG CGCGGAGCTT GGAGGCCTCC TTTGGGGCCA AGACTACGAG
    TTCAAAGTGA GACCATCCTC TGGCCGGGCT CAAGGCCCTG ACAGCAACGT GCTGCTCCTG
    AGGCTGCCCG AGCAAGTGCC CAGTGCCCCT CCCCAGGAGG TGGCCTTAAA ACCTGGCAAT
    GGCAGTGTCC TTGTAAGCTG GGTCCCACCA CCTGCTGAAA ACCACAATGG CATCATCCGT
    GGCTACCAGA TCTGGAGCCT GGGCAACACC TCGCTGCCCC CCGCCAACTG GACCGTGGCC
    GGTGAGCAGA CCCAGCTGGA GATCGCCACC CGAATGCCAG GTTCCTACTG TGTACAAGTG
    GCTGCAGTCA CTGGCGCCGG GACTGGGCCT CCCAGTAGCC CTGTCTGCCT CCTTTTAGAG
    CGGACCATGG AGCGAGCCAC CCAAGAGCTC AGTGATGGTC CCTGGACTCT GGAGCAGCTG
    AGGGCCACCT TGAGGCGGCC AGAAGTGATT GCTGGTGGGG GTGTCCTGCT CTGGCTGGTG
    CTGCTGGGCA CCGCCGTGTG TGTACACCGC AGGCGCCGAG CTGGGGTGCA CCTGGGCCCA
    GGTTTGTACA GGTACACCAG TGAGGATGCC ATCCTAAAGC ACAGGATGGA TCATAGTGAC
    TCCCCTTGGC TGGCAGACAC CTGGCGTTCC ACCTCCGGCT CTCGGGACCT GAGCAGCAGC
    AGCAGCCTCA ACAGCAGGCT GGGAGTGGAC CCCCGGGACC CACTAGACTG TCGCCGCTCC
    CTGATCTCCT GGGATCCCCG AAGCCCTGGT GTGCCCCTGC TTCCCGACAC CAGCACTTTT
    TATGGCTCCC TCATCGCTGA GCTGCCTGCC GGCCCCCCGG CCCGGCCAAG CCCCAAGACC
    CCAGCTGTCA GGCGTCTCCC ACCCCAGCTG GCCCGGCTCT CCAGCCCCTG GCCCAGTCCC
    GACAGCCTCG GCAGCCGCAG GGGCCTCGCT TCTCCTCTGG CCCCTGCAGA GGCTTGGAAG
    GCCAAAAAGA AGCAGGAGCT GCAACAAGCC AATAGCTCCC CGCCCCTCCG GGCCAGCCGC
    CCTATGGAGC TCTGGGCCTG GGAGTTGGGG AACAGAGGCT CCAAGAACCT TTCCCAAAGC
    CCAGGCTTTT GCTCCCCAGG AGCTGTGCCC CAGGCTCTGG TGGCCTGGAG GGCCCTGGGA
    CCGCAGCTCC TCGGCTCCTC CAATGAGATG GTTTCATGCC CTCTCCCCTC AGCACCCCTC
    GCTCCGCGTG GAGCCCCTAC TCAGAGTCAG CAGACCCAGC ACTCTGCAGA GCCCCAAGCT
    CCGTCCTCCC ACCCACTGCC AGCAGCCCCC ATCCTGATCC CCTCTAGCCC CCAGGCTTCT
    TCCCTCTCCG GGCCCAGCCC AGAGTCCAGT CGTCTGTCCA GCTCTTCACT GTCATCCCTG
    GAGGAGGATC AGGACAGCGT GCTGACTCCT GAGGAGGTGG CCCTGTGCCT GGAGCTCAGC
    GAGGGTGAGG AGACCCCCAG GAATAGTGTC TCTCCTATGC CAAGGGCTCC TTCACCCCCT
    GTCACCTACG GCTACATCAG CATCCCGGCT GCCTCAGAGC TGGCAGACAT GGGCAGGGCC
    GGAGGAGGGG TGGGATCCGA AGTGAGGGGC TTGCTGTGCC CGCCCCGGCC CTGCCCCACC
    CCTACCCCCA GCGAGGGCTC CCTGGCCAAT GGCTGGGGTT CAGCCTCCGA GGACAATGCC
    CCCAGTGCCC GAGCCAGCCT CGTCAGCTCC TCCGATGGCT CCTTCCTTGC TGATGCTCAC
    TTTGCGCGGG CCCTGGCGAT GGCTGTGGAC AGCTTCGGCT TTGGTCTGGA GCCCAGGGAC
    GTGGACTGCG TCTTCACGGA TGCCTCCTCT CCTCCTTCCC CCCGCGATGA CCTCTTCCTG
    ACCTCCACCC TCTCCCTGCC CCTGTGGGAG TGGAGGTCAG ACTGGCTGGA GGACATGGAG
    AACAACCGTA GCCAGCGGCT GGGAAGGGGG CTGCCTCCCT GGCCCCCTGA TTCCCGGCGC
    TCTTCCAGAA GGAGTCAGCT GGGCCGTCCT GAGCCCAAGG CCGCCAACTC CTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05769
    Clone ID Related Accession (Same CDS sequence) XM_546424.6 , XM_546424.5
    Accession Version XM_546424.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_546424.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGCTCTG GAGGAGCAGG CCTCCTTGGG CCCCTGGGGC CCCTGCCTCT CCTGCTTCTG 
    CTTGTCACGG GAGGCACGGC TCAGGACTCC CCACCCCAGA TCCTAGTCCA CCCCCAGGAC
    CAGCTGCTCC AGGGCCCTGG CCCCGCCAAA ATGAGCTGCC GAGCCTCGGG CCAGCCACCC
    CCCACCATCC GCTGGCTGCT GAATGGGCAA CCCCTGAGCA TGGTGCCCCC AGACGTCCAC
    CACCTCCTTC CTGATGGGAC GCTCCTGCTG CTGCGGCCCC CTGCCCGGGG ACGTGCCCAC
    GACGACCAGG CCCCATCCAC AGACCTGGGC ATCTACACGT GTGAGGCCAG CAACAGGCTG
    GGCACAGCAG TCAGCCGAGG AGCTCGGCTG TCTGTGGCTG TCCTTCGGGA GGACTTCCAG
    ACCCAGCCTC AGGATACAGT GGCCACTGTG GGCGAGCAGG TGATTCTGGA GTGTGGGCCG
    CCCTTGGGCC ATCCAGAGCC CACAGTCTCG TGGTGGAAAG ATGGCAAACC CCTGGCCCTA
    CGGCCAGGGC GGCACTCGGT CTCCAGGGGC TCCCTGCAGA TGGCAAGAGC AGAGAAGAGC
    GATGCAGGGA CCTATATGTG CGTGGCCACC AACAGCGCAG GACAACGGGA GAGCCGGGCA
    GCCAGGGTAT CTGTCCAGGA GCCCCAGGAC TACAAGAAGC CTCTGGAGCT GCTGGCCGTG
    CACATTCAGC TGGAAAATGT GACCTTGCTG AACCCAGACC CCGCAAAGAG CGCCAAGCCT
    GAGCCTGCTG TGTGGCTCAG CTGGAAGGTG AGCGGCCCTG CAGCACCTGC TCAGTCCTAC
    ACAGCCCTCT TCAGGGTCCA GACGGCCTCA GGAGGCCCGG GAGCTCCATG GGCAGAGGCC
    CTGCTGGCTG GCTGGCAAAG CGCGGAGCTT GGAGGCCTCC TTTGGGGCCA AGACTACGAG
    TTCAAAGTGA GACCATCCTC TGGCCGGGCT CAAGGCCCTG ACAGCAACGT GCTGCTCCTG
    AGGCTGCCCG AGCAAGTGCC CAGTGCCCCT CCCCAGGAGG TGGCCTTAAA ACCTGGCAAT
    GGCAGTGTCC TTGTAAGCTG GGTCCCACCA CCTGCTGAAA ACCACAATGG CATCATCCGT
    GGCTACCAGA TCTGGAGCCT GGGCAACACC TCGCTGCCCC CCGCCAACTG GACCGTGGCC
    GGTGAGCAGA CCCAGCTGGA GATCGCCACC CGAATGCCAG GTTCCTACTG TGTACAAGTG
    GCTGCAGTCA CTGGCGCCGG GACTGGGCCT CCCAGTAGCC CTGTCTGCCT CCTTTTAGAG
    CGGACCATGG AGCGAGCCAC CCAAGAGCTC AGTGATGGTC CCTGGACTCT GGAGCAGCTG
    AGGGCCACCT TGAGGCGGCC AGAAGTGATT GCTGGTGGGG GTGTCCTGCT CTGGCTGGTG
    CTGCTGGGCA CCGCCGTGTG TGTACACCGC AGGCGCCGAG CTGGGGTGCA CCTGGGCCCA
    GGTTTGTACA GGTACACCAG TGAGGATGCC ATCCTAAAGC ACAGGATGGA TCATAGTGAC
    TCCCCTTGGC TGGCAGACAC CTGGCGTTCC ACCTCCGGCT CTCGGGACCT GAGCAGCAGC
    AGCAGCCTCA ACAGCAGGCT GGGAGTGGAC CCCCGGGACC CACTAGACTG TCGCCGCTCC
    CTGATCTCCT GGGATCCCCG AAGCCCTGGT GTGCCCCTGC TTCCCGACAC CAGCACTTTT
    TATGGCTCCC TCATCGCTGA GCTGCCTGCC GGCCCCCCGG CCCGGCCAAG CCCCAAGACC
    CCAGCTGTCA GGCGTCTCCC ACCCCAGCTG GCCCGGCTCT CCAGCCCCTG GCCCAGTCCC
    GACAGCCTCG GCAGCCGCAG GGGCCTCGCT TCTCCTCTGG CCCCTGCAGA GGCTTGGAAG
    GCCAAAAAGA AGCAGGAGCT GCAACAAGCC AATAGCTCCC CGCCCCTCCG GGCCAGCCGC
    CCTATGGAGC TCTGGGCCTG GGAGTTGGGG AACAGAGGCT CCAAGAACCT TTCCCAAAGC
    CCAGGAGCTG TGCCCCAGGC TCTGGTGGCC TGGAGGGCCC TGGGACCGCA GCTCCTCGGC
    TCCTCCAATG AGATGGTTTC ATGCCCTCTC CCCTCAGCAC CCCTCGCTCC GCGTGGAGCC
    CCTACTCAGA GTCAGCAGAC CCAGCACTCT GCAGAGCCCC AAGCTCCGTC CTCCCACCCA
    CTGCCAGCAG CCCCCATCCT GATCCCCTCT AGCCCCCAGG CTTCTTCCCT CTCCGGGCCC
    AGCCCAGAGT CCAGTCGTCT GTCCAGCTCT TCACTGTCAT CCCTGGAGGA GGATCAGGAC
    AGCGTGCTGA CTCCTGAGGA GGTGGCCCTG TGCCTGGAGC TCAGCGAGGG TGAGGAGACC
    CCCAGGAATA GTGTCTCTCC TATGCCAAGG GCTCCTTCAC CCCCTGTCAC CTACGGCTAC
    ATCAGCATCC CGGCTGCCTC AGAGCTGGCA GACATGGGCA GGGCCGGAGG AGGGGTGGGA
    TCCGAAGTGA GGGGCTTGCT GTGCCCGCCC CGGCCCTGCC CCACCCCTAC CCCCAGCGAG
    GGCTCCCTGG CCAATGGCTG GGGTTCAGCC TCCGAGGACA ATGCCCCCAG TGCCCGAGCC
    AGCCTCGTCA GCTCCTCCGA TGGCTCCTTC CTTGCTGATG CTCACTTTGC GCGGGCCCTG
    GCGATGGCTG TGGACAGCTT CGGCTTTGGT CTGGAGCCCA GGGACGTGGA CTGCGTCTTC
    ACGGATGCCT CCTCTCCTCC TTCCCCCCGC GATGACCTCT TCCTGACCTC CACCCTCTCC
    CTGCCCCTGT GGGAGTGGAG GTCAGACTGG CTGGAGGACA TGGAGAACAA CCGTAGCCAG
    CGGCTGGGAA GGGGGCTGCC TCCCTGGCCC CCTGATTCCC GGCGCTCTTC CAGAAGGAGT
    CAGCTGGGCC GTCCTGAGCC CAAGGCCGCC AACTCCTCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_546424.3
    CDS124..3105
    Translation

    Target ORF information:

    RefSeq Version XM_546424.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 4 (ROBO4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_546424.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGCTCTG GAGGAGCAGG CCTCCTTGGG CCCCTGGGGC CCCTGCCTCT CCTGCTTCTG 
    CTTGTCACGG GAGGCACGGC TCAGGACTCC CCACCCCAGA TCCTAGTCCA CCCCCAGGAC
    CAGCTGCTCC AGGGCCCTGG CCCCGCCAAA ATGAGCTGCC GAGCCTCGGG CCAGCCACCC
    CCCACCATCC GCTGGCTGCT GAATGGGCAA CCCCTGAGCA TGGTGCCCCC AGACGTCCAC
    CACCTCCTTC CTGATGGGAC GCTCCTGCTG CTGCGGCCCC CTGCCCGGGG ACGTGCCCAC
    GACGACCAGG CCCCATCCAC AGACCTGGGC ATCTACACGT GTGAGGCCAG CAACAGGCTG
    GGCACAGCAG TCAGCCGAGG AGCTCGGCTG TCTGTGGCTG TCCTTCGGGA GGACTTCCAG
    ACCCAGCCTC AGGATACAGT GGCCACTGTG GGCGAGCAGG TGATTCTGGA GTGTGGGCCG
    CCCTTGGGCC ATCCAGAGCC CACAGTCTCG TGGTGGAAAG ATGGCAAACC CCTGGCCCTA
    CGGCCAGGGC GGCACTCGGT CTCCAGGGGC TCCCTGCAGA TGGCAAGAGC AGAGAAGAGC
    GATGCAGGGA CCTATATGTG CGTGGCCACC AACAGCGCAG GACAACGGGA GAGCCGGGCA
    GCCAGGGTAT CTGTCCAGGA GCCCCAGGAC TACAAGAAGC CTCTGGAGCT GCTGGCCGTG
    CACATTCAGC TGGAAAATGT GACCTTGCTG AACCCAGACC CCGCAAAGAG CGCCAAGCCT
    GAGCCTGCTG TGTGGCTCAG CTGGAAGGTG AGCGGCCCTG CAGCACCTGC TCAGTCCTAC
    ACAGCCCTCT TCAGGGTCCA GACGGCCTCA GGAGGCCCGG GAGCTCCATG GGCAGAGGCC
    CTGCTGGCTG GCTGGCAAAG CGCGGAGCTT GGAGGCCTCC TTTGGGGCCA AGACTACGAG
    TTCAAAGTGA GACCATCCTC TGGCCGGGCT CAAGGCCCTG ACAGCAACGT GCTGCTCCTG
    AGGCTGCCCG AGCAAGTGCC CAGTGCCCCT CCCCAGGAGG TGGCCTTAAA ACCTGGCAAT
    GGCAGTGTCC TTGTAAGCTG GGTCCCACCA CCTGCTGAAA ACCACAATGG CATCATCCGT
    GGCTACCAGA TCTGGAGCCT GGGCAACACC TCGCTGCCCC CCGCCAACTG GACCGTGGCC
    GGTGAGCAGA CCCAGCTGGA GATCGCCACC CGAATGCCAG GTTCCTACTG TGTACAAGTG
    GCTGCAGTCA CTGGCGCCGG GACTGGGCCT CCCAGTAGCC CTGTCTGCCT CCTTTTAGAG
    CGGACCATGG AGCGAGCCAC CCAAGAGCTC AGTGATGGTC CCTGGACTCT GGAGCAGCTG
    AGGGCCACCT TGAGGCGGCC AGAAGTGATT GCTGGTGGGG GTGTCCTGCT CTGGCTGGTG
    CTGCTGGGCA CCGCCGTGTG TGTACACCGC AGGCGCCGAG CTGGGGTGCA CCTGGGCCCA
    GGTTTGTACA GGTACACCAG TGAGGATGCC ATCCTAAAGC ACAGGATGGA TCATAGTGAC
    TCCCCTTGGC TGGCAGACAC CTGGCGTTCC ACCTCCGGCT CTCGGGACCT GAGCAGCAGC
    AGCAGCCTCA ACAGCAGGCT GGGAGTGGAC CCCCGGGACC CACTAGACTG TCGCCGCTCC
    CTGATCTCCT GGGATCCCCG AAGCCCTGGT GTGCCCCTGC TTCCCGACAC CAGCACTTTT
    TATGGCTCCC TCATCGCTGA GCTGCCTGCC GGCCCCCCGG CCCGGCCAAG CCCCAAGACC
    CCAGCTGTCA GGCGTCTCCC ACCCCAGCTG GCCCGGCTCT CCAGCCCCTG GCCCAGTCCC
    GACAGCCTCG GCAGCCGCAG GGGCCTCGCT TCTCCTCTGG CCCCTGCAGA GGCTTGGAAG
    GCCAAAAAGA AGCAGGAGCT GCAACAAGCC AATAGCTCCC CGCCCCTCCG GGCCAGCCGC
    CCTATGGAGC TCTGGGCCTG GGAGTTGGGG AACAGAGGCT CCAAGAACCT TTCCCAAAGC
    CCAGGAGCTG TGCCCCAGGC TCTGGTGGCC TGGAGGGCCC TGGGACCGCA GCTCCTCGGC
    TCCTCCAATG AGATGGTTTC ATGCCCTCTC CCCTCAGCAC CCCTCGCTCC GCGTGGAGCC
    CCTACTCAGA GTCAGCAGAC CCAGCACTCT GCAGAGCCCC AAGCTCCGTC CTCCCACCCA
    CTGCCAGCAG CCCCCATCCT GATCCCCTCT AGCCCCCAGG CTTCTTCCCT CTCCGGGCCC
    AGCCCAGAGT CCAGTCGTCT GTCCAGCTCT TCACTGTCAT CCCTGGAGGA GGATCAGGAC
    AGCGTGCTGA CTCCTGAGGA GGTGGCCCTG TGCCTGGAGC TCAGCGAGGG TGAGGAGACC
    CCCAGGAATA GTGTCTCTCC TATGCCAAGG GCTCCTTCAC CCCCTGTCAC CTACGGCTAC
    ATCAGCATCC CGGCTGCCTC AGAGCTGGCA GACATGGGCA GGGCCGGAGG AGGGGTGGGA
    TCCGAAGTGA GGGGCTTGCT GTGCCCGCCC CGGCCCTGCC CCACCCCTAC CCCCAGCGAG
    GGCTCCCTGG CCAATGGCTG GGGTTCAGCC TCCGAGGACA ATGCCCCCAG TGCCCGAGCC
    AGCCTCGTCA GCTCCTCCGA TGGCTCCTTC CTTGCTGATG CTCACTTTGC GCGGGCCCTG
    GCGATGGCTG TGGACAGCTT CGGCTTTGGT CTGGAGCCCA GGGACGTGGA CTGCGTCTTC
    ACGGATGCCT CCTCTCCTCC TTCCCCCCGC GATGACCTCT TCCTGACCTC CACCCTCTCC
    CTGCCCCTGT GGGAGTGGAG GTCAGACTGG CTGGAGGACA TGGAGAACAA CCGTAGCCAG
    CGGCTGGGAA GGGGGCTGCC TCCCTGGCCC CCTGATTCCC GGCGCTCTTC CAGAAGGAGT
    CAGCTGGGCC GTCCTGAGCC CAAGGCCGCC AACTCCTCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05769
    Clone ID Related Accession (Same CDS sequence) XM_546424.6 , XM_546424.5
    Accession Version XM_546424.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 4 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_546424.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGCTCTG GAGGAGCAGG CCTCCTTGGG CCCCTGGGGC CCCTGCCTCT CCTGCTTCTG 
    CTTGTCACGG GAGGCACGGC TCAGGACTCC CCACCCCAGA TCCTAGTCCA CCCCCAGGAC
    CAGCTGCTCC AGGGCCCTGG CCCCGCCAAA ATGAGCTGCC GAGCCTCGGG CCAGCCACCC
    CCCACCATCC GCTGGCTGCT GAATGGGCAA CCCCTGAGCA TGGTGCCCCC AGACGTCCAC
    CACCTCCTTC CTGATGGGAC GCTCCTGCTG CTGCGGCCCC CTGCCCGGGG ACGTGCCCAC
    GACGACCAGG CCCCATCCAC AGACCTGGGC ATCTACACGT GTGAGGCCAG CAACAGGCTG
    GGCACAGCAG TCAGCCGAGG AGCTCGGCTG TCTGTGGCTG TCCTTCGGGA GGACTTCCAG
    ACCCAGCCTC AGGATACAGT GGCCACTGTG GGCGAGCAGG TGATTCTGGA GTGTGGGCCG
    CCCTTGGGCC ATCCAGAGCC CACAGTCTCG TGGTGGAAAG ATGGCAAACC CCTGGCCCTA
    CGGCCAGGGC GGCACTCGGT CTCCAGGGGC TCCCTGCAGA TGGCAAGAGC AGAGAAGAGC
    GATGCAGGGA CCTATATGTG CGTGGCCACC AACAGCGCAG GACAACGGGA GAGCCGGGCA
    GCCAGGGTAT CTGTCCAGGA GCCCCAGGAC TACAAGAAGC CTCTGGAGCT GCTGGCCGTG
    CACATTCAGC TGGAAAATGT GACCTTGCTG AACCCAGACC CCGCAAAGAG CGCCAAGCCT
    GAGCCTGCTG TGTGGCTCAG CTGGAAGGTG AGCGGCCCTG CAGCACCTGC TCAGTCCTAC
    ACAGCCCTCT TCAGGGTCCA GACGGCCTCA GGAGGCCCGG GAGCTCCATG GGCAGAGGCC
    CTGCTGGCTG GCTGGCAAAG CGCGGAGCTT GGAGGCCTCC TTTGGGGCCA AGACTACGAG
    TTCAAAGTGA GACCATCCTC TGGCCGGGCT CAAGGCCCTG ACAGCAACGT GCTGCTCCTG
    AGGCTGCCCG AGCAAGTGCC CAGTGCCCCT CCCCAGGAGG TGGCCTTAAA ACCTGGCAAT
    GGCAGTGTCC TTGTAAGCTG GGTCCCACCA CCTGCTGAAA ACCACAATGG CATCATCCGT
    GGCTACCAGA TCTGGAGCCT GGGCAACACC TCGCTGCCCC CCGCCAACTG GACCGTGGCC
    GGTGAGCAGA CCCAGCTGGA GATCGCCACC CGAATGCCAG GTTCCTACTG TGTACAAGTG
    GCTGCAGTCA CTGGCGCCGG GACTGGGCCT CCCAGTAGCC CTGTCTGCCT CCTTTTAGAG
    CGGACCATGG AGCGAGCCAC CCAAGAGCTC AGTGATGGTC CCTGGACTCT GGAGCAGCTG
    AGGGCCACCT TGAGGCGGCC AGAAGTGATT GCTGGTGGGG GTGTCCTGCT CTGGCTGGTG
    CTGCTGGGCA CCGCCGTGTG TGTACACCGC AGGCGCCGAG CTGGGGTGCA CCTGGGCCCA
    GGTTTGTACA GGTACACCAG TGAGGATGCC ATCCTAAAGC ACAGGATGGA TCATAGTGAC
    TCCCCTTGGC TGGCAGACAC CTGGCGTTCC ACCTCCGGCT CTCGGGACCT GAGCAGCAGC
    AGCAGCCTCA ACAGCAGGCT GGGAGTGGAC CCCCGGGACC CACTAGACTG TCGCCGCTCC
    CTGATCTCCT GGGATCCCCG AAGCCCTGGT GTGCCCCTGC TTCCCGACAC CAGCACTTTT
    TATGGCTCCC TCATCGCTGA GCTGCCTGCC GGCCCCCCGG CCCGGCCAAG CCCCAAGACC
    CCAGCTGTCA GGCGTCTCCC ACCCCAGCTG GCCCGGCTCT CCAGCCCCTG GCCCAGTCCC
    GACAGCCTCG GCAGCCGCAG GGGCCTCGCT TCTCCTCTGG CCCCTGCAGA GGCTTGGAAG
    GCCAAAAAGA AGCAGGAGCT GCAACAAGCC AATAGCTCCC CGCCCCTCCG GGCCAGCCGC
    CCTATGGAGC TCTGGGCCTG GGAGTTGGGG AACAGAGGCT CCAAGAACCT TTCCCAAAGC
    CCAGGAGCTG TGCCCCAGGC TCTGGTGGCC TGGAGGGCCC TGGGACCGCA GCTCCTCGGC
    TCCTCCAATG AGATGGTTTC ATGCCCTCTC CCCTCAGCAC CCCTCGCTCC GCGTGGAGCC
    CCTACTCAGA GTCAGCAGAC CCAGCACTCT GCAGAGCCCC AAGCTCCGTC CTCCCACCCA
    CTGCCAGCAG CCCCCATCCT GATCCCCTCT AGCCCCCAGG CTTCTTCCCT CTCCGGGCCC
    AGCCCAGAGT CCAGTCGTCT GTCCAGCTCT TCACTGTCAT CCCTGGAGGA GGATCAGGAC
    AGCGTGCTGA CTCCTGAGGA GGTGGCCCTG TGCCTGGAGC TCAGCGAGGG TGAGGAGACC
    CCCAGGAATA GTGTCTCTCC TATGCCAAGG GCTCCTTCAC CCCCTGTCAC CTACGGCTAC
    ATCAGCATCC CGGCTGCCTC AGAGCTGGCA GACATGGGCA GGGCCGGAGG AGGGGTGGGA
    TCCGAAGTGA GGGGCTTGCT GTGCCCGCCC CGGCCCTGCC CCACCCCTAC CCCCAGCGAG
    GGCTCCCTGG CCAATGGCTG GGGTTCAGCC TCCGAGGACA ATGCCCCCAG TGCCCGAGCC
    AGCCTCGTCA GCTCCTCCGA TGGCTCCTTC CTTGCTGATG CTCACTTTGC GCGGGCCCTG
    GCGATGGCTG TGGACAGCTT CGGCTTTGGT CTGGAGCCCA GGGACGTGGA CTGCGTCTTC
    ACGGATGCCT CCTCTCCTCC TTCCCCCCGC GATGACCTCT TCCTGACCTC CACCCTCTCC
    CTGCCCCTGT GGGAGTGGAG GTCAGACTGG CTGGAGGACA TGGAGAACAA CCGTAGCCAG
    CGGCTGGGAA GGGGGCTGCC TCCCTGGCCC CCTGATTCCC GGCGCTCTTC CAGAAGGAGT
    CAGCTGGGCC GTCCTGAGCC CAAGGCCGCC AACTCCTCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_546424.3
    CDS118..3099
    Translation

    Target ORF information:

    RefSeq Version XM_546424.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 4 (ROBO4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_546424.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGGCTCTG GAGGAGCAGG CCTCCTTGGG CCCCTGGGGC CCCTGCCTCT CCTGCTTCTG 
    CTTGTCACGG GAGGCACGGC TCAGGACTCC CCACCCCAGA TCCTAGTCCA CCCCCAGGAC
    CAGCTGCTCC AGGGCCCTGG CCCCGCCAAA ATGAGCTGCC GAGCCTCGGG CCAGCCACCC
    CCCACCATCC GCTGGCTGCT GAATGGGCAA CCCCTGAGCA TGGTGCCCCC AGACGTCCAC
    CACCTCCTTC CTGATGGGAC GCTCCTGCTG CTGCGGCCCC CTGCCCGGGG ACGTGCCCAC
    GACGACCAGG CCCCATCCAC AGACCTGGGC ATCTACACGT GTGAGGCCAG CAACAGGCTG
    GGCACAGCAG TCAGCCGAGG AGCTCGGCTG TCTGTGGCTG TCCTTCGGGA GGACTTCCAG
    ACCCAGCCTC AGGATACAGT GGCCACTGTG GGCGAGCAGG TGATTCTGGA GTGTGGGCCG
    CCCTTGGGCC ATCCAGAGCC CACAGTCTCG TGGTGGAAAG ATGGCAAACC CCTGGCCCTA
    CGGCCAGGGC GGCACTCGGT CTCCAGGGGC TCCCTGCAGA TGGCAAGAGC AGAGAAGAGC
    GATGCAGGGA CCTATATGTG CGTGGCCACC AACAGCGCAG GACAACGGGA GAGCCGGGCA
    GCCAGGGTAT CTGTCCAGGA GCCCCAGGAC TACAAGAAGC CTCTGGAGCT GCTGGCCGTG
    CACATTCAGC TGGAAAATGT GACCTTGCTG AACCCAGACC CCGCAAAGAG CGCCAAGCCT
    GAGCCTGCTG TGTGGCTCAG CTGGAAGGTG AGCGGCCCTG CAGCACCTGC TCAGTCCTAC
    ACAGCCCTCT TCAGGGTCCA GACGGCCTCA GGAGGCCCGG GAGCTCCATG GGCAGAGGCC
    CTGCTGGCTG GCTGGCAAAG CGCGGAGCTT GGAGGCCTCC TTTGGGGCCA AGACTACGAG
    TTCAAAGTGA GACCATCCTC TGGCCGGGCT CAAGGCCCTG ACAGCAACGT GCTGCTCCTG
    AGGCTGCCCG AGCAAGTGCC CAGTGCCCCT CCCCAGGAGG TGGCCTTAAA ACCTGGCAAT
    GGCAGTGTCC TTGTAAGCTG GGTCCCACCA CCTGCTGAAA ACCACAATGG CATCATCCGT
    GGCTACCAGA TCTGGAGCCT GGGCAACACC TCGCTGCCCC CCGCCAACTG GACCGTGGCC
    GGTGAGCAGA CCCAGCTGGA GATCGCCACC CGAATGCCAG GTTCCTACTG TGTACAAGTG
    GCTGCAGTCA CTGGCGCCGG GACTGGGCCT CCCAGTAGCC CTGTCTGCCT CCTTTTAGAG
    CGGACCATGG AGCGAGCCAC CCAAGAGCTC AGTGATGGTC CCTGGACTCT GGAGCAGCTG
    AGGGCCACCT TGAGGCGGCC AGAAGTGATT GCTGGTGGGG GTGTCCTGCT CTGGCTGGTG
    CTGCTGGGCA CCGCCGTGTG TGTACACCGC AGGCGCCGAG CTGGGGTGCA CCTGGGCCCA
    GGTTTGTACA GGTACACCAG TGAGGATGCC ATCCTAAAGC ACAGGATGGA TCATAGTGAC
    TCCCCTTGGC TGGCAGACAC CTGGCGTTCC ACCTCCGGCT CTCGGGACCT GAGCAGCAGC
    AGCAGCCTCA ACAGCAGGCT GGGAGTGGAC CCCCGGGACC CACTAGACTG TCGCCGCTCC
    CTGATCTCCT GGGATCCCCG AAGCCCTGGT GTGCCCCTGC TTCCCGACAC CAGCACTTTT
    TATGGCTCCC TCATCGCTGA GCTGCCTGCC GGCCCCCCGG CCCGGCCAAG CCCCAAGACC
    CCAGCTGTCA GGCGTCTCCC ACCCCAGCTG GCCCGGCTCT CCAGCCCCTG GCCCAGTCCC
    GACAGCCTCG GCAGCCGCAG GGGCCTCGCT TCTCCTCTGG CCCCTGCAGA GGCTTGGAAG
    GCCAAAAAGA AGCAGGAGCT GCAACAAGCC AATAGCTCCC CGCCCCTCCG GGCCAGCCGC
    CCTATGGAGC TCTGGGCCTG GGAGTTGGGG AACAGAGGCT CCAAGAACCT TTCCCAAAGC
    CCAGGAGCTG TGCCCCAGGC TCTGGTGGCC TGGAGGGCCC TGGGACCGCA GCTCCTCGGC
    TCCTCCAATG AGATGGTTTC ATGCCCTCTC CCCTCAGCAC CCCTCGCTCC GCGTGGAGCC
    CCTACTCAGA GTCAGCAGAC CCAGCACTCT GCAGAGCCCC AAGCTCCGTC CTCCCACCCA
    CTGCCAGCAG CCCCCATCCT GATCCCCTCT AGCCCCCAGG CTTCTTCCCT CTCCGGGCCC
    AGCCCAGAGT CCAGTCGTCT GTCCAGCTCT TCACTGTCAT CCCTGGAGGA GGATCAGGAC
    AGCGTGCTGA CTCCTGAGGA GGTGGCCCTG TGCCTGGAGC TCAGCGAGGG TGAGGAGACC
    CCCAGGAATA GTGTCTCTCC TATGCCAAGG GCTCCTTCAC CCCCTGTCAC CTACGGCTAC
    ATCAGCATCC CGGCTGCCTC AGAGCTGGCA GACATGGGCA GGGCCGGAGG AGGGGTGGGA
    TCCGAAGTGA GGGGCTTGCT GTGCCCGCCC CGGCCCTGCC CCACCCCTAC CCCCAGCGAG
    GGCTCCCTGG CCAATGGCTG GGGTTCAGCC TCCGAGGACA ATGCCCCCAG TGCCCGAGCC
    AGCCTCGTCA GCTCCTCCGA TGGCTCCTTC CTTGCTGATG CTCACTTTGC GCGGGCCCTG
    GCGATGGCTG TGGACAGCTT CGGCTTTGGT CTGGAGCCCA GGGACGTGGA CTGCGTCTTC
    ACGGATGCCT CCTCTCCTCC TTCCCCCCGC GATGACCTCT TCCTGACCTC CACCCTCTCC
    CTGCCCCTGT GGGAGTGGAG GTCAGACTGG CTGGAGGACA TGGAGAACAA CCGTAGCCAG
    CGGCTGGGAA GGGGGCTGCC TCCCTGGCCC CCTGATTCCC GGCGCTCTTC CAGAAGGAGT
    CAGCTGGGCC GTCCTGAGCC CAAGGCCGCC AACTCCTCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05770
    Clone ID Related Accession (Same CDS sequence) XM_005619615.2 , XM_005619615.3
    Accession Version XM_005619615.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2847bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product roundabout homolog 4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619615.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCTGCC GAGCCTCGGG CCAGCCACCC CCCACCATCC GCTGGCTGCT GAATGGGCAA 
    CCCCTGAGCA TGGTGCCCCC AGACGTCCAC CACCTCCTTC CTGATGGGAC GCTCCTGCTG
    CTGCGGCCCC CTGCCCGGGG ACGTGCCCAC GACGACCAGG CCCCATCCAC AGACCTGGGC
    ATCTACACGT GTGAGGCCAG CAACAGGCTG GGCACAGCAG TCAGCCGAGG AGCTCGGCTG
    TCTGTGGCTG TCCTTCGGGA GGACTTCCAG ACCCAGCCTC AGGATACAGT GGCCACTGTG
    GGCGAGCAGG TGATTCTGGA GTGTGGGCCG CCCTTGGGCC ATCCAGAGCC CACAGTCTCG
    TGGTGGAAAG ATGGCAAACC CCTGGCCCTA CGGCCAGGGC GGCACTCGGT CTCCAGGGGC
    TCCCTGCAGA TGGCAAGAGC AGAGAAGAGC GATGCAGGGA CCTATATGTG CGTGGCCACC
    AACAGCGCAG GACAACGGGA GAGCCGGGCA GCCAGGGTAT CTGTCCAGGA GCCCCAGGAC
    TACAAGAAGC CTCTGGAGCT GCTGGCCGTG CACATTCAGC TGGAAAATGT GACCTTGCTG
    AACCCAGACC CCGCAAAGAG CGCCAAGCCT GAGCCTGCTG TGTGGCTCAG CTGGAAGGTG
    AGCGGCCCTG CAGCACCTGC TCAGTCCTAC ACAGCCCTCT TCAGGGTCCA GACGGCCTCA
    GGAGGCCCGG GAGCTCCATG GGCAGAGGCC CTGCTGGCTG GCTGGCAAAG CGCGGAGCTT
    GGAGGCCTCC TTTGGGGCCA AGACTACGAG TTCAAAGTGA GACCATCCTC TGGCCGGGCT
    CAAGGCCCTG ACAGCAACGT GCTGCTCCTG AGGCTGCCCG AGCAAGTGCC CAGTGCCCCT
    CCCCAGGAGG TGGCCTTAAA ACCTGGCAAT GGCAGTGTCC TTGTAAGCTG GGTCCCACCA
    CCTGCTGAAA ACCACAATGG CATCATCCGT GGCTACCAGA TCTGGAGCCT GGGCAACACC
    TCGCTGCCCC CCGCCAACTG GACCGTGGCC GGTGAGCAGA CCCAGCTGGA GATCGCCACC
    CGAATGCCAG GTTCCTACTG TGTACAAGTG GCTGCAGTCA CTGGCGCCGG GACTGGGCCT
    CCCAGTAGCC CTGTCTGCCT CCTTTTAGAG CGGACCATGG AGCGAGCCAC CCAAGAGCTC
    AGTGATGGTC CCTGGACTCT GGAGCAGCTG AGGGCCACCT TGAGGCGGCC AGAAGTGATT
    GCTGGTGGGG GTGTCCTGCT CTGGCTGGTG CTGCTGGGCA CCGCCGTGTG TGTACACCGC
    AGGCGCCGAG CTGGGGTGCA CCTGGGCCCA GGTTTGTACA GGTACACCAG TGAGGATGCC
    ATCCTAAAGC ACAGGATGGA TCATAGTGAC TCCCCTTGGC TGGCAGACAC CTGGCGTTCC
    ACCTCCGGCT CTCGGGACCT GAGCAGCAGC AGCAGCCTCA ACAGCAGGCT GGGAGTGGAC
    CCCCGGGACC CACTAGACTG TCGCCGCTCC CTGATCTCCT GGGATCCCCG AAGCCCTGGT
    GTGCCCCTGC TTCCCGACAC CAGCACTTTT TATGGCTCCC TCATCGCTGA GCTGCCTGCC
    GGCCCCCCGG CCCGGCCAAG CCCCAAGACC CCAGCTGTCA GGCGTCTCCC ACCCCAGCTG
    GCCCGGCTCT CCAGCCCCTG GCCCAGTCCC GACAGCCTCG GCAGCCGCAG GGGCCTCGCT
    TCTCCTCTGG CCCCTGCAGA GGCTTGGAAG GCCAAAAAGA AGCAGGAGCT GCAACAAGCC
    AATAGCTCCC CGCCCCTCCG GGCCAGCCGC CCTATGGAGC TCTGGGCCTG GGAGTTGGGG
    AACAGAGGCT CCAAGAACCT TTCCCAAAGC CCAGGCTTTT GCTCCCCAGG AGCTGTGCCC
    CAGGCTCTGG TGGCCTGGAG GGCCCTGGGA CCGCAGCTCC TCGGCTCCTC CAATGAGATG
    GTTTCATGCC CTCTCCCCTC AGCACCCCTC GCTCCGCGTG GAGCCCCTAC TCAGAGTCAG
    CAGACCCAGC ACTCTGCAGA GCCCCAAGCT CCGTCCTCCC ACCCACTGCC AGCAGCCCCC
    ATCCTGATCC CCTCTAGCCC CCAGGCTTCT TCCCTCTCCG GGCCCAGCCC AGAGTCCAGT
    CGTCTGTCCA GCTCTTCACT GTCATCCCTG GAGGAGGATC AGGACAGCGT GCTGACTCCT
    GAGGAGGTGG CCCTGTGCCT GGAGCTCAGC GAGGGTGAGG AGACCCCCAG GAATAGTGTC
    TCTCCTATGC CAAGGGCTCC TTCACCCCCT GTCACCTACG GCTACATCAG CATCCCGGCT
    GCCTCAGAGC TGGCAGACAT GGGCAGGGCC GGAGGAGGGG TGGGATCCGA AGTGAGGGGC
    TTGCTGTGCC CGCCCCGGCC CTGCCCCACC CCTACCCCCA GCGAGGGCTC CCTGGCCAAT
    GGCTGGGGTT CAGCCTCCGA GGACAATGCC CCCAGTGCCC GAGCCAGCCT CGTCAGCTCC
    TCCGATGGCT CCTTCCTTGC TGATGCTCAC TTTGCGCGGG CCCTGGCGAT GGCTGTGGAC
    AGCTTCGGCT TTGGTCTGGA GCCCAGGGAC GTGGACTGCG TCTTCACGGA TGCCTCCTCT
    CCTCCTTCCC CCCGCGATGA CCTCTTCCTG ACCTCCACCC TCTCCCTGCC CCTGTGGGAG
    TGGAGGTCAG ACTGGCTGGA GGACATGGAG AACAACCGTA GCCAGCGGCT GGGAAGGGGG
    CTGCCTCCCT GGCCCCCTGA TTCCCGGCGC TCTTCCAGAA GGAGTCAGCT GGGCCGTCCT
    GAGCCCAAGG CCGCCAACTC CTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619672.1
    CDS985..3831
    Translation

    Target ORF information:

    RefSeq Version XM_005619615.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris roundabout guidance receptor 4 (ROBO4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619615.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCTGCC GAGCCTCGGG CCAGCCACCC CCCACCATCC GCTGGCTGCT GAATGGGCAA 
    CCCCTGAGCA TGGTGCCCCC AGACGTCCAC CACCTCCTTC CTGATGGGAC GCTCCTGCTG
    CTGCGGCCCC CTGCCCGGGG ACGTGCCCAC GACGACCAGG CCCCATCCAC AGACCTGGGC
    ATCTACACGT GTGAGGCCAG CAACAGGCTG GGCACAGCAG TCAGCCGAGG AGCTCGGCTG
    TCTGTGGCTG TCCTTCGGGA GGACTTCCAG ACCCAGCCTC AGGATACAGT GGCCACTGTG
    GGCGAGCAGG TGATTCTGGA GTGTGGGCCG CCCTTGGGCC ATCCAGAGCC CACAGTCTCG
    TGGTGGAAAG ATGGCAAACC CCTGGCCCTA CGGCCAGGGC GGCACTCGGT CTCCAGGGGC
    TCCCTGCAGA TGGCAAGAGC AGAGAAGAGC GATGCAGGGA CCTATATGTG CGTGGCCACC
    AACAGCGCAG GACAACGGGA GAGCCGGGCA GCCAGGGTAT CTGTCCAGGA GCCCCAGGAC
    TACAAGAAGC CTCTGGAGCT GCTGGCCGTG CACATTCAGC TGGAAAATGT GACCTTGCTG
    AACCCAGACC CCGCAAAGAG CGCCAAGCCT GAGCCTGCTG TGTGGCTCAG CTGGAAGGTG
    AGCGGCCCTG CAGCACCTGC TCAGTCCTAC ACAGCCCTCT TCAGGGTCCA GACGGCCTCA
    GGAGGCCCGG GAGCTCCATG GGCAGAGGCC CTGCTGGCTG GCTGGCAAAG CGCGGAGCTT
    GGAGGCCTCC TTTGGGGCCA AGACTACGAG TTCAAAGTGA GACCATCCTC TGGCCGGGCT
    CAAGGCCCTG ACAGCAACGT GCTGCTCCTG AGGCTGCCCG AGCAAGTGCC CAGTGCCCCT
    CCCCAGGAGG TGGCCTTAAA ACCTGGCAAT GGCAGTGTCC TTGTAAGCTG GGTCCCACCA
    CCTGCTGAAA ACCACAATGG CATCATCCGT GGCTACCAGA TCTGGAGCCT GGGCAACACC
    TCGCTGCCCC CCGCCAACTG GACCGTGGCC GGTGAGCAGA CCCAGCTGGA GATCGCCACC
    CGAATGCCAG GTTCCTACTG TGTACAAGTG GCTGCAGTCA CTGGCGCCGG GACTGGGCCT
    CCCAGTAGCC CTGTCTGCCT CCTTTTAGAG CGGACCATGG AGCGAGCCAC CCAAGAGCTC
    AGTGATGGTC CCTGGACTCT GGAGCAGCTG AGGGCCACCT TGAGGCGGCC AGAAGTGATT
    GCTGGTGGGG GTGTCCTGCT CTGGCTGGTG CTGCTGGGCA CCGCCGTGTG TGTACACCGC
    AGGCGCCGAG CTGGGGTGCA CCTGGGCCCA GGTTTGTACA GGTACACCAG TGAGGATGCC
    ATCCTAAAGC ACAGGATGGA TCATAGTGAC TCCCCTTGGC TGGCAGACAC CTGGCGTTCC
    ACCTCCGGCT CTCGGGACCT GAGCAGCAGC AGCAGCCTCA ACAGCAGGCT GGGAGTGGAC
    CCCCGGGACC CACTAGACTG TCGCCGCTCC CTGATCTCCT GGGATCCCCG AAGCCCTGGT
    GTGCCCCTGC TTCCCGACAC CAGCACTTTT TATGGCTCCC TCATCGCTGA GCTGCCTGCC
    GGCCCCCCGG CCCGGCCAAG CCCCAAGACC CCAGCTGTCA GGCGTCTCCC ACCCCAGCTG
    GCCCGGCTCT CCAGCCCCTG GCCCAGTCCC GACAGCCTCG GCAGCCGCAG GGGCCTCGCT
    TCTCCTCTGG CCCCTGCAGA GGCTTGGAAG GCCAAAAAGA AGCAGGAGCT GCAACAAGCC
    AATAGCTCCC CGCCCCTCCG GGCCAGCCGC CCTATGGAGC TCTGGGCCTG GGAGTTGGGG
    AACAGAGGCT CCAAGAACCT TTCCCAAAGC CCAGGCTTTT GCTCCCCAGG AGCTGTGCCC
    CAGGCTCTGG TGGCCTGGAG GGCCCTGGGA CCGCAGCTCC TCGGCTCCTC CAATGAGATG
    GTTTCATGCC CTCTCCCCTC AGCACCCCTC GCTCCGCGTG GAGCCCCTAC TCAGAGTCAG
    CAGACCCAGC ACTCTGCAGA GCCCCAAGCT CCGTCCTCCC ACCCACTGCC AGCAGCCCCC
    ATCCTGATCC CCTCTAGCCC CCAGGCTTCT TCCCTCTCCG GGCCCAGCCC AGAGTCCAGT
    CGTCTGTCCA GCTCTTCACT GTCATCCCTG GAGGAGGATC AGGACAGCGT GCTGACTCCT
    GAGGAGGTGG CCCTGTGCCT GGAGCTCAGC GAGGGTGAGG AGACCCCCAG GAATAGTGTC
    TCTCCTATGC CAAGGGCTCC TTCACCCCCT GTCACCTACG GCTACATCAG CATCCCGGCT
    GCCTCAGAGC TGGCAGACAT GGGCAGGGCC GGAGGAGGGG TGGGATCCGA AGTGAGGGGC
    TTGCTGTGCC CGCCCCGGCC CTGCCCCACC CCTACCCCCA GCGAGGGCTC CCTGGCCAAT
    GGCTGGGGTT CAGCCTCCGA GGACAATGCC CCCAGTGCCC GAGCCAGCCT CGTCAGCTCC
    TCCGATGGCT CCTTCCTTGC TGATGCTCAC TTTGCGCGGG CCCTGGCGAT GGCTGTGGAC
    AGCTTCGGCT TTGGTCTGGA GCCCAGGGAC GTGGACTGCG TCTTCACGGA TGCCTCCTCT
    CCTCCTTCCC CCCGCGATGA CCTCTTCCTG ACCTCCACCC TCTCCCTGCC CCTGTGGGAG
    TGGAGGTCAG ACTGGCTGGA GGACATGGAG AACAACCGTA GCCAGCGGCT GGGAAGGGGG
    CTGCCTCCCT GGCCCCCTGA TTCCCGGCGC TCTTCCAGAA GGAGTCAGCT GGGCCGTCCT
    GAGCCCAAGG CCGCCAACTC CTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05770
    Clone ID Related Accession (Same CDS sequence) XM_005619615.2 , XM_005619615.3
    Accession Version XM_005619615.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2847bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619615.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCTGCC GAGCCTCGGG CCAGCCACCC CCCACCATCC GCTGGCTGCT GAATGGGCAA 
    CCCCTGAGCA TGGTGCCCCC AGACGTCCAC CACCTCCTTC CTGATGGGAC GCTCCTGCTG
    CTGCGGCCCC CTGCCCGGGG ACGTGCCCAC GACGACCAGG CCCCATCCAC AGACCTGGGC
    ATCTACACGT GTGAGGCCAG CAACAGGCTG GGCACAGCAG TCAGCCGAGG AGCTCGGCTG
    TCTGTGGCTG TCCTTCGGGA GGACTTCCAG ACCCAGCCTC AGGATACAGT GGCCACTGTG
    GGCGAGCAGG TGATTCTGGA GTGTGGGCCG CCCTTGGGCC ATCCAGAGCC CACAGTCTCG
    TGGTGGAAAG ATGGCAAACC CCTGGCCCTA CGGCCAGGGC GGCACTCGGT CTCCAGGGGC
    TCCCTGCAGA TGGCAAGAGC AGAGAAGAGC GATGCAGGGA CCTATATGTG CGTGGCCACC
    AACAGCGCAG GACAACGGGA GAGCCGGGCA GCCAGGGTAT CTGTCCAGGA GCCCCAGGAC
    TACAAGAAGC CTCTGGAGCT GCTGGCCGTG CACATTCAGC TGGAAAATGT GACCTTGCTG
    AACCCAGACC CCGCAAAGAG CGCCAAGCCT GAGCCTGCTG TGTGGCTCAG CTGGAAGGTG
    AGCGGCCCTG CAGCACCTGC TCAGTCCTAC ACAGCCCTCT TCAGGGTCCA GACGGCCTCA
    GGAGGCCCGG GAGCTCCATG GGCAGAGGCC CTGCTGGCTG GCTGGCAAAG CGCGGAGCTT
    GGAGGCCTCC TTTGGGGCCA AGACTACGAG TTCAAAGTGA GACCATCCTC TGGCCGGGCT
    CAAGGCCCTG ACAGCAACGT GCTGCTCCTG AGGCTGCCCG AGCAAGTGCC CAGTGCCCCT
    CCCCAGGAGG TGGCCTTAAA ACCTGGCAAT GGCAGTGTCC TTGTAAGCTG GGTCCCACCA
    CCTGCTGAAA ACCACAATGG CATCATCCGT GGCTACCAGA TCTGGAGCCT GGGCAACACC
    TCGCTGCCCC CCGCCAACTG GACCGTGGCC GGTGAGCAGA CCCAGCTGGA GATCGCCACC
    CGAATGCCAG GTTCCTACTG TGTACAAGTG GCTGCAGTCA CTGGCGCCGG GACTGGGCCT
    CCCAGTAGCC CTGTCTGCCT CCTTTTAGAG CGGACCATGG AGCGAGCCAC CCAAGAGCTC
    AGTGATGGTC CCTGGACTCT GGAGCAGCTG AGGGCCACCT TGAGGCGGCC AGAAGTGATT
    GCTGGTGGGG GTGTCCTGCT CTGGCTGGTG CTGCTGGGCA CCGCCGTGTG TGTACACCGC
    AGGCGCCGAG CTGGGGTGCA CCTGGGCCCA GGTTTGTACA GGTACACCAG TGAGGATGCC
    ATCCTAAAGC ACAGGATGGA TCATAGTGAC TCCCCTTGGC TGGCAGACAC CTGGCGTTCC
    ACCTCCGGCT CTCGGGACCT GAGCAGCAGC AGCAGCCTCA ACAGCAGGCT GGGAGTGGAC
    CCCCGGGACC CACTAGACTG TCGCCGCTCC CTGATCTCCT GGGATCCCCG AAGCCCTGGT
    GTGCCCCTGC TTCCCGACAC CAGCACTTTT TATGGCTCCC TCATCGCTGA GCTGCCTGCC
    GGCCCCCCGG CCCGGCCAAG CCCCAAGACC CCAGCTGTCA GGCGTCTCCC ACCCCAGCTG
    GCCCGGCTCT CCAGCCCCTG GCCCAGTCCC GACAGCCTCG GCAGCCGCAG GGGCCTCGCT
    TCTCCTCTGG CCCCTGCAGA GGCTTGGAAG GCCAAAAAGA AGCAGGAGCT GCAACAAGCC
    AATAGCTCCC CGCCCCTCCG GGCCAGCCGC CCTATGGAGC TCTGGGCCTG GGAGTTGGGG
    AACAGAGGCT CCAAGAACCT TTCCCAAAGC CCAGGCTTTT GCTCCCCAGG AGCTGTGCCC
    CAGGCTCTGG TGGCCTGGAG GGCCCTGGGA CCGCAGCTCC TCGGCTCCTC CAATGAGATG
    GTTTCATGCC CTCTCCCCTC AGCACCCCTC GCTCCGCGTG GAGCCCCTAC TCAGAGTCAG
    CAGACCCAGC ACTCTGCAGA GCCCCAAGCT CCGTCCTCCC ACCCACTGCC AGCAGCCCCC
    ATCCTGATCC CCTCTAGCCC CCAGGCTTCT TCCCTCTCCG GGCCCAGCCC AGAGTCCAGT
    CGTCTGTCCA GCTCTTCACT GTCATCCCTG GAGGAGGATC AGGACAGCGT GCTGACTCCT
    GAGGAGGTGG CCCTGTGCCT GGAGCTCAGC GAGGGTGAGG AGACCCCCAG GAATAGTGTC
    TCTCCTATGC CAAGGGCTCC TTCACCCCCT GTCACCTACG GCTACATCAG CATCCCGGCT
    GCCTCAGAGC TGGCAGACAT GGGCAGGGCC GGAGGAGGGG TGGGATCCGA AGTGAGGGGC
    TTGCTGTGCC CGCCCCGGCC CTGCCCCACC CCTACCCCCA GCGAGGGCTC CCTGGCCAAT
    GGCTGGGGTT CAGCCTCCGA GGACAATGCC CCCAGTGCCC GAGCCAGCCT CGTCAGCTCC
    TCCGATGGCT CCTTCCTTGC TGATGCTCAC TTTGCGCGGG CCCTGGCGAT GGCTGTGGAC
    AGCTTCGGCT TTGGTCTGGA GCCCAGGGAC GTGGACTGCG TCTTCACGGA TGCCTCCTCT
    CCTCCTTCCC CCCGCGATGA CCTCTTCCTG ACCTCCACCC TCTCCCTGCC CCTGTGGGAG
    TGGAGGTCAG ACTGGCTGGA GGACATGGAG AACAACCGTA GCCAGCGGCT GGGAAGGGGG
    CTGCCTCCCT GGCCCCCTGA TTCCCGGCGC TCTTCCAGAA GGAGTCAGCT GGGCCGTCCT
    GAGCCCAAGG CCGCCAACTC CTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619672.1
    CDS422..3268
    Translation

    Target ORF information:

    RefSeq Version XM_005619615.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 4 (ROBO4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619615.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGCTGCC GAGCCTCGGG CCAGCCACCC CCCACCATCC GCTGGCTGCT GAATGGGCAA 
    CCCCTGAGCA TGGTGCCCCC AGACGTCCAC CACCTCCTTC CTGATGGGAC GCTCCTGCTG
    CTGCGGCCCC CTGCCCGGGG ACGTGCCCAC GACGACCAGG CCCCATCCAC AGACCTGGGC
    ATCTACACGT GTGAGGCCAG CAACAGGCTG GGCACAGCAG TCAGCCGAGG AGCTCGGCTG
    TCTGTGGCTG TCCTTCGGGA GGACTTCCAG ACCCAGCCTC AGGATACAGT GGCCACTGTG
    GGCGAGCAGG TGATTCTGGA GTGTGGGCCG CCCTTGGGCC ATCCAGAGCC CACAGTCTCG
    TGGTGGAAAG ATGGCAAACC CCTGGCCCTA CGGCCAGGGC GGCACTCGGT CTCCAGGGGC
    TCCCTGCAGA TGGCAAGAGC AGAGAAGAGC GATGCAGGGA CCTATATGTG CGTGGCCACC
    AACAGCGCAG GACAACGGGA GAGCCGGGCA GCCAGGGTAT CTGTCCAGGA GCCCCAGGAC
    TACAAGAAGC CTCTGGAGCT GCTGGCCGTG CACATTCAGC TGGAAAATGT GACCTTGCTG
    AACCCAGACC CCGCAAAGAG CGCCAAGCCT GAGCCTGCTG TGTGGCTCAG CTGGAAGGTG
    AGCGGCCCTG CAGCACCTGC TCAGTCCTAC ACAGCCCTCT TCAGGGTCCA GACGGCCTCA
    GGAGGCCCGG GAGCTCCATG GGCAGAGGCC CTGCTGGCTG GCTGGCAAAG CGCGGAGCTT
    GGAGGCCTCC TTTGGGGCCA AGACTACGAG TTCAAAGTGA GACCATCCTC TGGCCGGGCT
    CAAGGCCCTG ACAGCAACGT GCTGCTCCTG AGGCTGCCCG AGCAAGTGCC CAGTGCCCCT
    CCCCAGGAGG TGGCCTTAAA ACCTGGCAAT GGCAGTGTCC TTGTAAGCTG GGTCCCACCA
    CCTGCTGAAA ACCACAATGG CATCATCCGT GGCTACCAGA TCTGGAGCCT GGGCAACACC
    TCGCTGCCCC CCGCCAACTG GACCGTGGCC GGTGAGCAGA CCCAGCTGGA GATCGCCACC
    CGAATGCCAG GTTCCTACTG TGTACAAGTG GCTGCAGTCA CTGGCGCCGG GACTGGGCCT
    CCCAGTAGCC CTGTCTGCCT CCTTTTAGAG CGGACCATGG AGCGAGCCAC CCAAGAGCTC
    AGTGATGGTC CCTGGACTCT GGAGCAGCTG AGGGCCACCT TGAGGCGGCC AGAAGTGATT
    GCTGGTGGGG GTGTCCTGCT CTGGCTGGTG CTGCTGGGCA CCGCCGTGTG TGTACACCGC
    AGGCGCCGAG CTGGGGTGCA CCTGGGCCCA GGTTTGTACA GGTACACCAG TGAGGATGCC
    ATCCTAAAGC ACAGGATGGA TCATAGTGAC TCCCCTTGGC TGGCAGACAC CTGGCGTTCC
    ACCTCCGGCT CTCGGGACCT GAGCAGCAGC AGCAGCCTCA ACAGCAGGCT GGGAGTGGAC
    CCCCGGGACC CACTAGACTG TCGCCGCTCC CTGATCTCCT GGGATCCCCG AAGCCCTGGT
    GTGCCCCTGC TTCCCGACAC CAGCACTTTT TATGGCTCCC TCATCGCTGA GCTGCCTGCC
    GGCCCCCCGG CCCGGCCAAG CCCCAAGACC CCAGCTGTCA GGCGTCTCCC ACCCCAGCTG
    GCCCGGCTCT CCAGCCCCTG GCCCAGTCCC GACAGCCTCG GCAGCCGCAG GGGCCTCGCT
    TCTCCTCTGG CCCCTGCAGA GGCTTGGAAG GCCAAAAAGA AGCAGGAGCT GCAACAAGCC
    AATAGCTCCC CGCCCCTCCG GGCCAGCCGC CCTATGGAGC TCTGGGCCTG GGAGTTGGGG
    AACAGAGGCT CCAAGAACCT TTCCCAAAGC CCAGGCTTTT GCTCCCCAGG AGCTGTGCCC
    CAGGCTCTGG TGGCCTGGAG GGCCCTGGGA CCGCAGCTCC TCGGCTCCTC CAATGAGATG
    GTTTCATGCC CTCTCCCCTC AGCACCCCTC GCTCCGCGTG GAGCCCCTAC TCAGAGTCAG
    CAGACCCAGC ACTCTGCAGA GCCCCAAGCT CCGTCCTCCC ACCCACTGCC AGCAGCCCCC
    ATCCTGATCC CCTCTAGCCC CCAGGCTTCT TCCCTCTCCG GGCCCAGCCC AGAGTCCAGT
    CGTCTGTCCA GCTCTTCACT GTCATCCCTG GAGGAGGATC AGGACAGCGT GCTGACTCCT
    GAGGAGGTGG CCCTGTGCCT GGAGCTCAGC GAGGGTGAGG AGACCCCCAG GAATAGTGTC
    TCTCCTATGC CAAGGGCTCC TTCACCCCCT GTCACCTACG GCTACATCAG CATCCCGGCT
    GCCTCAGAGC TGGCAGACAT GGGCAGGGCC GGAGGAGGGG TGGGATCCGA AGTGAGGGGC
    TTGCTGTGCC CGCCCCGGCC CTGCCCCACC CCTACCCCCA GCGAGGGCTC CCTGGCCAAT
    GGCTGGGGTT CAGCCTCCGA GGACAATGCC CCCAGTGCCC GAGCCAGCCT CGTCAGCTCC
    TCCGATGGCT CCTTCCTTGC TGATGCTCAC TTTGCGCGGG CCCTGGCGAT GGCTGTGGAC
    AGCTTCGGCT TTGGTCTGGA GCCCAGGGAC GTGGACTGCG TCTTCACGGA TGCCTCCTCT
    CCTCCTTCCC CCCGCGATGA CCTCTTCCTG ACCTCCACCC TCTCCCTGCC CCTGTGGGAG
    TGGAGGTCAG ACTGGCTGGA GGACATGGAG AACAACCGTA GCCAGCGGCT GGGAAGGGGG
    CTGCCTCCCT GGCCCCCTGA TTCCCGGCGC TCTTCCAGAA GGAGTCAGCT GGGCCGTCCT
    GAGCCCAAGG CCGCCAACTC CTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287891
    Clone ID Related Accession (Same CDS sequence) XM_022418150.1
    Accession Version XM_022418150.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product roundabout homolog 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCAAACC CCTGGCCCTA CGGCCAGGGC GGCACTCGGT CTCCAGGGGC TCCCTGCAGA 
    TGGCAAGAGC AGAGAAGAGC GATGCAGGGA CCTATATGTG CGTGGCCACC AACAGCGCAG
    GACAACGGGA GAGCCGGGCA GCCAGGGTAT CTGTCCAGGA GGCCCCAGAC CAGCCCCATG
    CTCCCCACAG AGCCCCAGGA CTACAAGAAG CCTCTGGAGC TGCTGGCCGT GCACATTCAG
    CTGGAAAATG TGACCTTGCT GAACCCAGAC CCCGCAAAGA GCGCCAAGCC TGAGCCTGCT
    GTGTGGCTCA GCTGGAAGGT GAGCGGCCCT GCAGCACCTG CTCAGTCCTA CACAGCCCTC
    TTCAGGGTCC AGACGGCCTC AGGAGGCCCG GGAGCTCCAT GGGCAGAGGC CCTGCTGGCT
    GGCTGGCAAA GCGCGGAGCT TGGAGGCCTC CTTTGGGGCC AAGACTACGA GTTCAAAGTG
    AGACCATCCT CTGGCCGGGC TCAAGGCCCT GACAGCAACG TGCTGCTCCT GAGGCTGCCC
    GAGCAAGTGC CCAGTGCCCC TCCCCAGGAG GTGGCCTTAA AACCTGGCAA TGGCAGTGTC
    CTTGTAAGCT GGGTCCCACC ACCTGCTGAA AACCACAATG GCATCATCCG TGGCTACCAG
    ATCTGGAGCC TGGGCAACAC CTCGCTGCCC CCCGCCAACT GGACCGTGGC CGGTGAGCAG
    ACCCAGCTGG AGATCGCCAC CCGAATGCCA GGTTCCTACT GTGTACAAGT GGCTGCAGTC
    ACTGGCGCCG GGACTGGGCC TCCCAGTAGC CCTGTCTGCC TCCTTTTAGA GCGGACCATG
    GAGCGAGCCA CCCAAGAGCT CAGTGATGGT CCCTGGACTC TGGAGCAGCT GAGGGCCACC
    TTGAGGCGGC CAGAAGTGAT TGCTGGTGGG GGTGTCCTGC TCTGGCTGGT GCTGCTGGGC
    ACCGCCGTGT GTGTACACCG CAGGCGCCGA GCTGGGGTGC ACCTGGGCCC AGGTTTGTAC
    AGGTACACCA GTGAGGATGC CATCCTAAAG CACAGGATGG ATCATAGTGA CTCCCCTTGG
    CTGGCAGACA CCTGGCGTTC CACCTCCGGC TCTCGGGACC TGAGCAGCAG CAGCAGCCTC
    AACAGCAGGC TGGGAGTGGA CCCCCGGGAC CCACTAGACT GTCGCCGCTC CCTGATCTCC
    TGGGATCCCC GAAGCCCTGG TGTGCCCCTG CTTCCCGACA CCAGCACTTT TTATGGCTCC
    CTCATCGCTG AGCTGCCTGC CGGCCCCCCG GCCCGGCCAA GCCCCAAGAC CCCAGCTGTC
    AGGCGTCTCC CACCCCAGCT GGCCCGGCTC TCCAGCCCCT GGCCCAGTCC CGACAGCCTC
    GGCAGCCGCA GGGGCCTCGC TTCTCCTCTG GCCCCTGCAG AGGCTTGGAA GGCCAAAAAG
    AAGCAGGAGC TGCAACAAGC CAATAGCTCC CCGCCCCTCC GGGCCAGCCG CCCTATGGAG
    CTCTGGGCCT GGGAGTTGGG GAACAGAGGC TCCAAGAACC TTTCCCAAAG CCCAGGCTTT
    TGCTCCCCAG GAGCTGTGCC CCAGGCTCTG GTGGCCTGGA GGGCCCTGGG ACCGCAGCTC
    CTCGGCTCCT CCAATGAGAT GGTTTCATGC CCTCTCCCCT CAGCACCCCT CGCTCCGCGT
    GGAGCCCCTA CTCAGAGTCA GCAGACCCAG CACTCTGCAG AGCCCCAAGC TCCGTCCTCC
    CACCCACTGC CAGCAGCCCC CATCCTGATC CCCTCTAGCC CCCAGGCTTC TTCCCTCTCC
    GGGCCCAGCC CAGAGTCCAG TCGTCTGTCC AGCTCTTCAC TGTCATCCCT GGAGGAGGAT
    CAGGACAGCG TGCTGACTCC TGAGGAGGTG GCCCTGTGCC TGGAGCTCAG CGAGGGTGAG
    GAGACCCCCA GGAATAGTGT CTCTCCTATG CCAAGGGCTC CTTCACCCCC TGTCACCTAC
    GGCTACATCA GCATCCCGGC TGCCTCAGAG CTGGCAGACA TGGGCAGGGC CGGAGGAGGG
    GTGGGATCCG AAGTGAGGGG CTTGCTGTGC CCGCCCCGGC CCTGCCCCAC CCCTACCCCC
    AGCGAGGGCT CCCTGGCCAA TGGCTGGGGT TCAGCCTCCG AGGACAATGC CCCCAGTGCC
    CGAGCCAGCC TCGTCAGCTC CTCCGATGGC TCCTTCCTTG CTGATGCTCA CTTTGCGCGG
    GCCCTGGCGA TGGCTGTGGA CAGCTTCGGC TTTGGTCTGG AGCCCAGGGA CGTGGACTGC
    GTCTTCACGG ATGCCTCCTC TCCTCCTTCC CCCCGCGATG ACCTCTTCCT GACCTCCACC
    CTCTCCCTGC CCCTGTGGGA GTGGAGGTCA GACTGGCTGG AGGACATGGA GAACAACCGT
    AGCCAGCGGC TGGGAAGGGG GCTGCCTCCC TGGCCCCCTG ATTCCCGGCG CTCTTCCAGA
    AGGAGTCAGC TGGGCCGTCC TGAGCCCAAG GCCGCCAACT CCTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273858.1
    CDS264..2771
    Translation

    Target ORF information:

    RefSeq Version XM_022418150.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris roundabout guidance receptor 4 (ROBO4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418150.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCAAACC CCTGGCCCTA CGGCCAGGGC GGCACTCGGT CTCCAGGGGC TCCCTGCAGA 
    TGGCAAGAGC AGAGAAGAGC GATGCAGGGA CCTATATGTG CGTGGCCACC AACAGCGCAG
    GACAACGGGA GAGCCGGGCA GCCAGGGTAT CTGTCCAGGA GGCCCCAGAC CAGCCCCATG
    CTCCCCACAG AGCCCCAGGA CTACAAGAAG CCTCTGGAGC TGCTGGCCGT GCACATTCAG
    CTGGAAAATG TGACCTTGCT GAACCCAGAC CCCGCAAAGA GCGCCAAGCC TGAGCCTGCT
    GTGTGGCTCA GCTGGAAGGT GAGCGGCCCT GCAGCACCTG CTCAGTCCTA CACAGCCCTC
    TTCAGGGTCC AGACGGCCTC AGGAGGCCCG GGAGCTCCAT GGGCAGAGGC CCTGCTGGCT
    GGCTGGCAAA GCGCGGAGCT TGGAGGCCTC CTTTGGGGCC AAGACTACGA GTTCAAAGTG
    AGACCATCCT CTGGCCGGGC TCAAGGCCCT GACAGCAACG TGCTGCTCCT GAGGCTGCCC
    GAGCAAGTGC CCAGTGCCCC TCCCCAGGAG GTGGCCTTAA AACCTGGCAA TGGCAGTGTC
    CTTGTAAGCT GGGTCCCACC ACCTGCTGAA AACCACAATG GCATCATCCG TGGCTACCAG
    ATCTGGAGCC TGGGCAACAC CTCGCTGCCC CCCGCCAACT GGACCGTGGC CGGTGAGCAG
    ACCCAGCTGG AGATCGCCAC CCGAATGCCA GGTTCCTACT GTGTACAAGT GGCTGCAGTC
    ACTGGCGCCG GGACTGGGCC TCCCAGTAGC CCTGTCTGCC TCCTTTTAGA GCGGACCATG
    GAGCGAGCCA CCCAAGAGCT CAGTGATGGT CCCTGGACTC TGGAGCAGCT GAGGGCCACC
    TTGAGGCGGC CAGAAGTGAT TGCTGGTGGG GGTGTCCTGC TCTGGCTGGT GCTGCTGGGC
    ACCGCCGTGT GTGTACACCG CAGGCGCCGA GCTGGGGTGC ACCTGGGCCC AGGTTTGTAC
    AGGTACACCA GTGAGGATGC CATCCTAAAG CACAGGATGG ATCATAGTGA CTCCCCTTGG
    CTGGCAGACA CCTGGCGTTC CACCTCCGGC TCTCGGGACC TGAGCAGCAG CAGCAGCCTC
    AACAGCAGGC TGGGAGTGGA CCCCCGGGAC CCACTAGACT GTCGCCGCTC CCTGATCTCC
    TGGGATCCCC GAAGCCCTGG TGTGCCCCTG CTTCCCGACA CCAGCACTTT TTATGGCTCC
    CTCATCGCTG AGCTGCCTGC CGGCCCCCCG GCCCGGCCAA GCCCCAAGAC CCCAGCTGTC
    AGGCGTCTCC CACCCCAGCT GGCCCGGCTC TCCAGCCCCT GGCCCAGTCC CGACAGCCTC
    GGCAGCCGCA GGGGCCTCGC TTCTCCTCTG GCCCCTGCAG AGGCTTGGAA GGCCAAAAAG
    AAGCAGGAGC TGCAACAAGC CAATAGCTCC CCGCCCCTCC GGGCCAGCCG CCCTATGGAG
    CTCTGGGCCT GGGAGTTGGG GAACAGAGGC TCCAAGAACC TTTCCCAAAG CCCAGGCTTT
    TGCTCCCCAG GAGCTGTGCC CCAGGCTCTG GTGGCCTGGA GGGCCCTGGG ACCGCAGCTC
    CTCGGCTCCT CCAATGAGAT GGTTTCATGC CCTCTCCCCT CAGCACCCCT CGCTCCGCGT
    GGAGCCCCTA CTCAGAGTCA GCAGACCCAG CACTCTGCAG AGCCCCAAGC TCCGTCCTCC
    CACCCACTGC CAGCAGCCCC CATCCTGATC CCCTCTAGCC CCCAGGCTTC TTCCCTCTCC
    GGGCCCAGCC CAGAGTCCAG TCGTCTGTCC AGCTCTTCAC TGTCATCCCT GGAGGAGGAT
    CAGGACAGCG TGCTGACTCC TGAGGAGGTG GCCCTGTGCC TGGAGCTCAG CGAGGGTGAG
    GAGACCCCCA GGAATAGTGT CTCTCCTATG CCAAGGGCTC CTTCACCCCC TGTCACCTAC
    GGCTACATCA GCATCCCGGC TGCCTCAGAG CTGGCAGACA TGGGCAGGGC CGGAGGAGGG
    GTGGGATCCG AAGTGAGGGG CTTGCTGTGC CCGCCCCGGC CCTGCCCCAC CCCTACCCCC
    AGCGAGGGCT CCCTGGCCAA TGGCTGGGGT TCAGCCTCCG AGGACAATGC CCCCAGTGCC
    CGAGCCAGCC TCGTCAGCTC CTCCGATGGC TCCTTCCTTG CTGATGCTCA CTTTGCGCGG
    GCCCTGGCGA TGGCTGTGGA CAGCTTCGGC TTTGGTCTGG AGCCCAGGGA CGTGGACTGC
    GTCTTCACGG ATGCCTCCTC TCCTCCTTCC CCCCGCGATG ACCTCTTCCT GACCTCCACC
    CTCTCCCTGC CCCTGTGGGA GTGGAGGTCA GACTGGCTGG AGGACATGGA GAACAACCGT
    AGCCAGCGGC TGGGAAGGGG GCTGCCTCCC TGGCCCCCTG ATTCCCGGCG CTCTTCCAGA
    AGGAGTCAGC TGGGCCGTCC TGAGCCCAAG GCCGCCAACT CCTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.