robo4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following robo4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the robo4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa62262 XM_002937520.4
Latest version!
Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa62263 XM_012966984.2
Latest version!
Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa62264 XM_004916033.4
Latest version!
Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa13010 XM_002937520.3
Latest version!
Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa13011 XM_004916033.3
Latest version!
Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa35654 XM_012966984.1
Latest version!
Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa62262
    Clone ID Related Accession (Same CDS sequence) XM_002937520.4
    Accession Version XM_002937520.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937520.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAATTTTG CCTACTTTCT TCCAATTTTT GTACTTTTTC CCTTGGGATG GTGTTCACAG 
    GTGAGCTCCT GCCAATGTCA TCAGCCAGTC ATTAATAAAG AACATGGCCT GGAAGCTACA
    CGTAGACAGA CCCGGCATGT TCAGCACCAC CGGTCAACAA GAAGGCACCG TATTAGAGGC
    TCAAAAACTC GATCAGAGGA TTTTCCACCT CGTATTATCG AACACCCTTC AGATTTGTTT
    GTGAAGAAGG ACAAGCCAGC CACATTGTAT TGTCGAGCAG AGGGCAATCC ACAGCCCACA
    ATAGAATGGT ACCGTAATGG AGAGCATGTG GAGACCAGCA CAGATGATAA GACCCAGCGT
    TCCTATGTTC AGTTGCCGGA GGGACCACTC TTCTTCTTCA GCCTTAATCA ACGAAAAGGC
    AAATCTGATG AAGGGATTTA TACTTGTGTG GCTAGAAACC ATCTAGGAAC GGCATTTAGT
    AGAAATGCTT CTCTTTATGT TGCTGCTCTA AGGGATGATT TTCGTCTAAA TCCATCTGAT
    GCTGTTGTGG CCGTGGGGAA GCAACTTGAG ATAGAATGTT TGCCTCCAAA AGGTCATCCT
    GAGCCCAATG TTACTTGGAA GAAGGATGGG ATTCCAATAA ACCATAATAA TAGTCGATAC
    ACGATTTCTG CAGGCAAACT CTTTATTGGC AAAGCACTAA AGACGGATTC AGGACTATAT
    TCCTGCTTAG CATCAAATCA AGTGGGAGAA AGAGAGAGTA GAGCTGCTAA AATCGTTGTA
    CTGGAGAAGC CCATGTTTAC ATATAAACCC AGTGATGTGA CGGCAAAGTT TGGAAGCACC
    GTGCAGTTTG GTTGTGGAGC ACAAGGAGAC CCAAAGCCCA CCTTTCACTG GAGCAAAGAA
    AATGGAGACC TTCCACAAGG GAGGTATGAA ATTACCAATG AAAATACATT AAGAATCCGA
    TCTGTAACCT TGTATGACTC TGGAAAATAT ATCTGCACTG CAGGAAATCA GCTAGAAACA
    GTATCTGCCA AGGCATTGCT AACTGTTCAA GATCCTTTGG ATACTGGTCA GATAGAAAAG
    GAGAGACAAA TCTATAGAGA ATTAATGGGT GTTCGTGTTC ATTTAGACAA CATCACAGCA
    CTTCCAAGCG CATCAGTCTA TGTGCACTGG CAGGTCCTAA CGGAATCCCC CTTTACTGAA
    GGATATAGTG TATTTTACCG CACACTTTCT TCTACTGAAT CTGAGTGGAC AGAGTGGACA
    AAGTCTAGGA TCAATGAGTA CAGCACTGTT ATTCCATCTC TATGGAGGGG ACAGAAATAT
    GAGTTTAAGA TCCGACCTTT TGCTGGGAAA AATTATGGAC CTGACAGCAA TATTAAACAT
    CTCAGAATAC CTGAGGAAGT TCCAAATATT GCTCCACAGA ACATCAATGT GACTGCTGTG
    GAAGGAGGAA ACGGTACAAT AGTTGTGAGC TGGGAACCCC CACCAAAAAA CATTCACAGC
    AGTAACATAA AAGGTTATAA GGTCTGGTGT TTTGAGAATG AAACACATCC TAAAGCAGAC
    TGGATAGTGG ATGAAGAAAC CAAGAGTCTA GAAATTCCCA TGTTGGCCTC CGGCATTAAA
    TATCAAGTCC AAGTAGCGGC TATAAATGAT GCAGGAATTG GAATGCCAAG CATACCTAAA
    TACATAATGA TAGACTCACC AGAAGTGAAG GAGGTAAAAG ATGAAAATAT ATATTTTGAG
    CTAATCCTGC AAGTTGTGCG TCACCCAGCA TTCATTGGAA CTGTAGGAAC CGTGGCCTGG
    ATATTATTAA TGGTTGCAGC CGTGTATGTG TGTCAGCGTC ATTCTAAGCG CTATAGCACA
    AAACAACACA GTATATTAGG AAACAGTCTT TACCGGTTTG CGAGTGAAGA TACCATAATA
    AAGCACAGGA TGGACATTAG TGATTCCCCA TGGCTTTCAC ACACATGGAA ATCCGCCTCC
    TGCTCGCGAA ACTACAGCAG CACAATCAGT ATGAACAGCC AATTACTGTG GTCTGAAACT
    AAAGACACGG CAGATTTCCA TAAATCAACA ATGTCGTTTG AGCGCAAAAG TGAGGGTAGT
    CGAAGCCAGA TTATTCCGCT GGTGCCAGAC AGCAACAGCT TGTATGGAAC AATGTATGTC
    GACCTTCCAG GAAAAGATAT GACCACATTT CAGCGTCTGC CTTCTGACAA ATCTCAAGGC
    ATGGGGAGGC ATTTTAAAAC TCCTGACCCT CTAGGATTGC TTCATCACAG GCCATTGCCA
    TCATATTTGA ATGATTTCAG TGGCAGCAAT GATATGAATG GAGGAACTAG AAGCAAGTTG
    CCATGGCAAC CAAATATTAA CAATAATCCT GATATCTCAT TTAAGGACTC ATGGAGCAAA
    AACTGCAAGA AAGAGCTTCA ACATGCAAAT AGTGCTCCTT TGTCCCCTTA TTGCCAAGCA
    CCCAAACAGG GTGACAGTGT ACCAAGCATT CAGCATTTAA ACATCAAGAA AGTAGGAAAA
    GGGGACTATG CTAAAGTAAT GAAGACTTTC AGTTCCCCTA AAATCCTTCA CTACACCACC
    TCTCTCCAAG TGATGGATCT TCTACCTTAT ACACCTTCTC TCCCACCACC TCCAGTTCCT
    CCTCCAGATG AAAATCCAAT TCACAATGAC AAGGATAAAA TAAGTGGTTG CAGTGTCTCA
    TCATATTCCG TGCGCAATCA AGAAAAGCAA GGCCATAAAA TCAACAGCAA GAAAAAGAGT
    CCCCCTGATA CACTGAACAT TAAACCAGCT TCAGAATGCT TCCCAATAAA TGATGATGGA
    GATAATGTGT TAACTCCAGA AGATGTGGCA CAATATCTTG AACTCAGTGA TCAAGGGCAA
    AATACAAGGC ATAAATCAGA ATATGATGCT TCCCTTCCAA GGCCTTTCTC TCCTTCTCAA
    ACATATGGCT ACATTTGCAG CCCGATACCT TCAGAAGCTG ATGTTGTTGA TGAAGATGAT
    GACCTTGATT TAGAGGACAT CGGTTCATTA AAGTCTTATC GCAAGTACTG TGAAACTCCA
    ACCTCCAGTA TCAGTGAATA TGAGAGCTCT ATGGCCGGAT CTCTTGTTAA TGGCTGGGGT
    TCTGTTTCAG AAGATAATTA TACCAGTGCT AGATGCAGCA TGGTCAGCTC CTCTGATGGG
    TCCTTTTTAA TGGATGCAAA CTTCGCCAAG GCTCTTGCAG TTGCAGTTGA CAGTTTTTGC
    TTTGGTGGAT CTCAGACTGA GACTGCTGGA AGAGATACGT TTTATGCAGA TCTTTCTCCG
    TCAGTGTCTC CTTTGGATGG AATATTAAAC CAACATAGTG GGGAAAATAC AGATGTTACC
    AAAAAGAAAT CCAGAGTTAA TCATTTGCCT GTTTTAGACT GGAACATTGA CTGGATGGAT
    GAAATGGAAG CCAAGTACAG CAGGAAAAAT GACATGAAAC ATCATTTTCC TTTTGATAAA
    AAGATGGATC CTTTCAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937566.3
    CDS143..3643
    Translation

    Target ORF information:

    RefSeq Version XM_002937520.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937520.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAATTTTG CCTACTTTCT TCCAATTTTT GTACTTTTTC CCTTGGGATG GTGTTCACAG 
    GTGAGCTCCT GCCAATGTCA TCAGCCAGTC ATTAATAAAG AACATGGCCT GGAAGCTACA
    CGTAGACAGA CCCGGCATGT TCAGCACCAC CGGTCAACAA GAAGGCACCG TATTAGAGGC
    TCAAAAACTC GATCAGAGGA TTTTCCACCT CGTATTATCG AACACCCTTC AGATTTGTTT
    GTGAAGAAGG ACAAGCCAGC CACATTGTAT TGTCGAGCAG AGGGCAATCC ACAGCCCACA
    ATAGAATGGT ACCGTAATGG AGAGCATGTG GAGACCAGCA CAGATGATAA GACCCAGCGT
    TCCTATGTTC AGTTGCCGGA GGGACCACTC TTCTTCTTCA GCCTTAATCA ACGAAAAGGC
    AAATCTGATG AAGGGATTTA TACTTGTGTG GCTAGAAACC ATCTAGGAAC GGCATTTAGT
    AGAAATGCTT CTCTTTATGT TGCTGCTCTA AGGGATGATT TTCGTCTAAA TCCATCTGAT
    GCTGTTGTGG CCGTGGGGAA GCAACTTGAG ATAGAATGTT TGCCTCCAAA AGGTCATCCT
    GAGCCCAATG TTACTTGGAA GAAGGATGGG ATTCCAATAA ACCATAATAA TAGTCGATAC
    ACGATTTCTG CAGGCAAACT CTTTATTGGC AAAGCACTAA AGACGGATTC AGGACTATAT
    TCCTGCTTAG CATCAAATCA AGTGGGAGAA AGAGAGAGTA GAGCTGCTAA AATCGTTGTA
    CTGGAGAAGC CCATGTTTAC ATATAAACCC AGTGATGTGA CGGCAAAGTT TGGAAGCACC
    GTGCAGTTTG GTTGTGGAGC ACAAGGAGAC CCAAAGCCCA CCTTTCACTG GAGCAAAGAA
    AATGGAGACC TTCCACAAGG GAGGTATGAA ATTACCAATG AAAATACATT AAGAATCCGA
    TCTGTAACCT TGTATGACTC TGGAAAATAT ATCTGCACTG CAGGAAATCA GCTAGAAACA
    GTATCTGCCA AGGCATTGCT AACTGTTCAA GATCCTTTGG ATACTGGTCA GATAGAAAAG
    GAGAGACAAA TCTATAGAGA ATTAATGGGT GTTCGTGTTC ATTTAGACAA CATCACAGCA
    CTTCCAAGCG CATCAGTCTA TGTGCACTGG CAGGTCCTAA CGGAATCCCC CTTTACTGAA
    GGATATAGTG TATTTTACCG CACACTTTCT TCTACTGAAT CTGAGTGGAC AGAGTGGACA
    AAGTCTAGGA TCAATGAGTA CAGCACTGTT ATTCCATCTC TATGGAGGGG ACAGAAATAT
    GAGTTTAAGA TCCGACCTTT TGCTGGGAAA AATTATGGAC CTGACAGCAA TATTAAACAT
    CTCAGAATAC CTGAGGAAGT TCCAAATATT GCTCCACAGA ACATCAATGT GACTGCTGTG
    GAAGGAGGAA ACGGTACAAT AGTTGTGAGC TGGGAACCCC CACCAAAAAA CATTCACAGC
    AGTAACATAA AAGGTTATAA GGTCTGGTGT TTTGAGAATG AAACACATCC TAAAGCAGAC
    TGGATAGTGG ATGAAGAAAC CAAGAGTCTA GAAATTCCCA TGTTGGCCTC CGGCATTAAA
    TATCAAGTCC AAGTAGCGGC TATAAATGAT GCAGGAATTG GAATGCCAAG CATACCTAAA
    TACATAATGA TAGACTCACC AGAAGTGAAG GAGGTAAAAG ATGAAAATAT ATATTTTGAG
    CTAATCCTGC AAGTTGTGCG TCACCCAGCA TTCATTGGAA CTGTAGGAAC CGTGGCCTGG
    ATATTATTAA TGGTTGCAGC CGTGTATGTG TGTCAGCGTC ATTCTAAGCG CTATAGCACA
    AAACAACACA GTATATTAGG AAACAGTCTT TACCGGTTTG CGAGTGAAGA TACCATAATA
    AAGCACAGGA TGGACATTAG TGATTCCCCA TGGCTTTCAC ACACATGGAA ATCCGCCTCC
    TGCTCGCGAA ACTACAGCAG CACAATCAGT ATGAACAGCC AATTACTGTG GTCTGAAACT
    AAAGACACGG CAGATTTCCA TAAATCAACA ATGTCGTTTG AGCGCAAAAG TGAGGGTAGT
    CGAAGCCAGA TTATTCCGCT GGTGCCAGAC AGCAACAGCT TGTATGGAAC AATGTATGTC
    GACCTTCCAG GAAAAGATAT GACCACATTT CAGCGTCTGC CTTCTGACAA ATCTCAAGGC
    ATGGGGAGGC ATTTTAAAAC TCCTGACCCT CTAGGATTGC TTCATCACAG GCCATTGCCA
    TCATATTTGA ATGATTTCAG TGGCAGCAAT GATATGAATG GAGGAACTAG AAGCAAGTTG
    CCATGGCAAC CAAATATTAA CAATAATCCT GATATCTCAT TTAAGGACTC ATGGAGCAAA
    AACTGCAAGA AAGAGCTTCA ACATGCAAAT AGTGCTCCTT TGTCCCCTTA TTGCCAAGCA
    CCCAAACAGG GTGACAGTGT ACCAAGCATT CAGCATTTAA ACATCAAGAA AGTAGGAAAA
    GGGGACTATG CTAAAGTAAT GAAGACTTTC AGTTCCCCTA AAATCCTTCA CTACACCACC
    TCTCTCCAAG TGATGGATCT TCTACCTTAT ACACCTTCTC TCCCACCACC TCCAGTTCCT
    CCTCCAGATG AAAATCCAAT TCACAATGAC AAGGATAAAA TAAGTGGTTG CAGTGTCTCA
    TCATATTCCG TGCGCAATCA AGAAAAGCAA GGCCATAAAA TCAACAGCAA GAAAAAGAGT
    CCCCCTGATA CACTGAACAT TAAACCAGCT TCAGAATGCT TCCCAATAAA TGATGATGGA
    GATAATGTGT TAACTCCAGA AGATGTGGCA CAATATCTTG AACTCAGTGA TCAAGGGCAA
    AATACAAGGC ATAAATCAGA ATATGATGCT TCCCTTCCAA GGCCTTTCTC TCCTTCTCAA
    ACATATGGCT ACATTTGCAG CCCGATACCT TCAGAAGCTG ATGTTGTTGA TGAAGATGAT
    GACCTTGATT TAGAGGACAT CGGTTCATTA AAGTCTTATC GCAAGTACTG TGAAACTCCA
    ACCTCCAGTA TCAGTGAATA TGAGAGCTCT ATGGCCGGAT CTCTTGTTAA TGGCTGGGGT
    TCTGTTTCAG AAGATAATTA TACCAGTGCT AGATGCAGCA TGGTCAGCTC CTCTGATGGG
    TCCTTTTTAA TGGATGCAAA CTTCGCCAAG GCTCTTGCAG TTGCAGTTGA CAGTTTTTGC
    TTTGGTGGAT CTCAGACTGA GACTGCTGGA AGAGATACGT TTTATGCAGA TCTTTCTCCG
    TCAGTGTCTC CTTTGGATGG AATATTAAAC CAACATAGTG GGGAAAATAC AGATGTTACC
    AAAAAGAAAT CCAGAGTTAA TCATTTGCCT GTTTTAGACT GGAACATTGA CTGGATGGAT
    GAAATGGAAG CCAAGTACAG CAGGAAAAAT GACATGAAAC ATCATTTTCC TTTTGATAAA
    AAGATGGATC CTTTCAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62263
    Clone ID Related Accession (Same CDS sequence) XM_012966984.2
    Accession Version XM_012966984.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966984.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAATTTTG CCTACTTTCT TCCAATTTTT GTACTTTTTC CCTTGGGATG GTGTTCACAG 
    GTGAGCTCCT GCCAATGTCA TCAGCCAGTC ATTAATAAAG AACATGGCCT GGAAGCTACA
    CGTAGACAGA CCCGGCATGT TCAGCACCAC CGGTCAACAA GAAGGCACCG TATTAGAGGC
    TCAAAAACTC GATCAGAGGA TTTTCCACCT CGTATTATCG AACACCCTTC AGATTTGTTT
    GTGAAGAAGG ACAAGCCAGC CACATTGTAT TGTCGAGCAG AGGGCAATCC ACAGCCCACA
    ATAGAATGGT ACCGTAATGG AGAGCATGTG GAGACCAGCA CAGATGATAA GACCCAGCGT
    TCCTATGTTC AGTTGCCGGA GGGACCACTC TTCTTCTTCA GCCTTAATCA ACGAAAAGGC
    AAATCTGATG AAGGGATTTA TACTTGTGTG GCTAGAAACC ATCTAGGAAC GGCATTTAGT
    AGAAATGCTT CTCTTTATGT TGCTGCTCTA AGGGATGATT TTCGTCTAAA TCCATCTGAT
    GCTGTTGTGG CCGTGGGGAA GCAACTTGAG ATAGAATGTT TGCCTCCAAA AGGTCATCCT
    GAGCCCAATG TTACTTGGAA GAAGGATGGG ATTCCAATAA ACCATAATAA TAGTCGATAC
    ACGATTTCTG CAGGCAAACT CTTTATTGGC AAAGCACTAA AGACGGATTC AGGACTATAT
    TCCTGCTTAG CATCAAATCA AGTGGGAGAA AGAGAGAGTA GAGCTGCTAA AATCGTTGTA
    CTGGAGAAGC CCATGTTTAC ATATAAACCC AGTGATGTGA CGGCAAAGTT TGGAAGCACC
    GTGCAGTTTG GTTGTGGAGC ACAAGGAGAC CCAAAGCCCA CCTTTCACTG GAGCAAAGAA
    AATGGAGACC TTCCACAAGG GAGGTATGAA ATTACCAATG AAAATACATT AAGAATCCGA
    TCTGTAACCT TGTATGACTC TGGAAAATAT ATCTGCACTG CAGGAAATCA GCTAGAAACA
    GTATCTGCCA AGGCATTGCT AACTGTTCAA GATCCTTTGG ATACTGGTCA GATAGAAAAG
    GAGAGACAAA TCTATAGAGA ATTAATGGGT GTTCGTGTTC ATTTAGACAA CATCACAGCA
    CTTCCAAGCG CATCAGTCTA TGTGCACTGG CAGGTCCTAA CGGAATCCCC CTTTACTGAA
    GGATATAGTG TATTTTACCG CACACTTTCT TCTACTGAAT CTGAGTGGAC AGAGTGGACA
    AAGTCTAGGA TCAATGAGTA CAGCACTGTT ATTCCATCTC TATGGAGGGG ACAGAAATAT
    GAGTTTAAGA TCCGACCTTT TGCTGGGAAA AATTATGGAC CTGACAGCAA TATTAAACAT
    CTCAGAATAC CTGAGGAAGT TCCAAATATT GCTCCACAGA ACATCAATGT GACTGCTGTG
    GAAGGAGGAA ACGGTACAAT AGTTGTGAGC TGGGAACCCC CACCAAAAAA CATTCACAGC
    AGTAACATAA AAGGTTATAA GGTCTGGTGT TTTGAGAATG AAACACATCC TAAAGCAGAC
    TGGATAGTGG ATGAAGAAAC CAAGAGTCTA GAAATTCCCA TGTTGGCCTC CGGCATTAAA
    TATCAAGTCC AAGTAGCGGC TATAAATGAT GCAGGAATTG GAATGCCAAG CATACCTAAA
    TACATAATGA TAGACTCACC AGAAGTGAAG GAGGTAAAAG ATGAAAATAT ATATTTTGAG
    CTAATCCTGC AAGTTGTGCG TCACCCAGCA TTCATTGGAA CTGTAGGAAC CGTGGCCTGG
    ATATTATTAA TGGTTGCAGC CGTGTATGTG TGTCAGCGTC ATTCTAAGCG CTATAGCACA
    AAACAACACA GTATATTAGG AAACAGTCTT TACCGGTTTG CGAGTGAAGA TACCATAATA
    AAGCACAGGA TGGACATTAG TGATTCCCCA TGGCTTTCAC ACACATGGAA ATCCGCCTCC
    TGCTCGCGAA ACTACAGCAG CACAATCAGT ATGAACAGCC AATTACTGTG GTCTGAAACT
    AAAGACACGG CAGATTTCCA TAAATCAACA ATGTCGTTTG AGCGCAAAAG TGAGGGTAGT
    CGAAGCCAGA TTATTCCGCT GGTGCCAGAC AGCAACAGCT TGTATGGAAC AATGTATGTC
    GACCTTCCAG GAAAAGATAT GACCACATTT CAGCGTCTGC CTTCTGACAA ATCTCAAGGC
    ATGGGGAGGC ATTTTAAAAC TCCTGACCCT CTAGGATTGC TTCATCACAG GCCATTGCCA
    TCATATTTGA ATGATTTCAG TGGCAGCAAT GATATGAATG GAGGAACTAG AAGCAAGTTG
    CCATGGCAAC CAAATATTAA CAATAATCCT GATATCTCAT TTAAGGACTC ATGGAGCAAA
    AACTGCAAGA AAGAGCTTCA ACATGCAAAT AGTGCTCCTT TGTCCCCTTA TTGCCAAGCA
    CCCAAACAGG GTGACAGTGT ACCAAGCATT CAGCATTTAA ACATCAAGAA AGTAGGAAAA
    GGGGACTATG CTAAAGTAAT GAAGACTTTC AGTTCCCCTA AAATCCTTCA CTACACCACC
    TCTCTCCAAG TGATGGATCT TCTACCTTAT ACACCTTCTC TCCCACCACC TCCAGTTCCT
    CCTCCAGATG AAAATCCAAT TCACAATGAC AAGGATAAAA TTGGTTGCAG TGTCTCATCA
    TATTCCGTGC GCAATCAAGA AAAGCAAGGC CATAAAATCA ACAGCAAGAA AAAGAGTCCC
    CCTGATACAC TGAACATTAA ACCAGCTTCA GAATGCTTCC CAATAAATGA TGATGGAGAT
    AATGTGTTAA CTCCAGAAGA TGTGGCACAA TATCTTGAAC TCAGTGATCA AGGGCAAAAT
    ACAAGGCATA AATCAGAATA TGATGCTTCC CTTCCAAGGC CTTTCTCTCC TTCTCAAACA
    TATGGCTACA TTTGCAGCCC GATACCTTCA GAAGCTGATG TTGTTGATGA AGATGATGAC
    CTTGATTTAG AGGACATCGG TTCATTAAAG TCTTATCGCA AGTACTGTGA AACTCCAACC
    TCCAGTATCA GTGAATATGA GAGCTCTATG GCCGGATCTC TTGTTAATGG CTGGGGTTCT
    GTTTCAGAAG ATAATTATAC CAGTGCTAGA TGCAGCATGG TCAGCTCCTC TGATGGGTCC
    TTTTTAATGG ATGCAAACTT CGCCAAGGCT CTTGCAGTTG CAGTTGACAG TTTTTGCTTT
    GGTGGATCTC AGACTGAGAC TGCTGGAAGA GATACGTTTT ATGCAGATCT TTCTCCGTCA
    GTGTCTCCTT TGGATGGAAT ATTAAACCAA CATAGTGGGG AAAATACAGA TGTTACCAAA
    AAGAAATCCA GAGTTAATCA TTTGCCTGTT TTAGACTGGA ACATTGACTG GATGGATGAA
    ATGGAAGCCA AGTACAGCAG GAAAAATGAC ATGAAACATC ATTTTCCTTT TGATAAAAAG
    ATGGATCCTT TCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822438.2
    CDS144..3641
    Translation

    Target ORF information:

    RefSeq Version XM_012966984.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966984.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAATTTTG CCTACTTTCT TCCAATTTTT GTACTTTTTC CCTTGGGATG GTGTTCACAG 
    GTGAGCTCCT GCCAATGTCA TCAGCCAGTC ATTAATAAAG AACATGGCCT GGAAGCTACA
    CGTAGACAGA CCCGGCATGT TCAGCACCAC CGGTCAACAA GAAGGCACCG TATTAGAGGC
    TCAAAAACTC GATCAGAGGA TTTTCCACCT CGTATTATCG AACACCCTTC AGATTTGTTT
    GTGAAGAAGG ACAAGCCAGC CACATTGTAT TGTCGAGCAG AGGGCAATCC ACAGCCCACA
    ATAGAATGGT ACCGTAATGG AGAGCATGTG GAGACCAGCA CAGATGATAA GACCCAGCGT
    TCCTATGTTC AGTTGCCGGA GGGACCACTC TTCTTCTTCA GCCTTAATCA ACGAAAAGGC
    AAATCTGATG AAGGGATTTA TACTTGTGTG GCTAGAAACC ATCTAGGAAC GGCATTTAGT
    AGAAATGCTT CTCTTTATGT TGCTGCTCTA AGGGATGATT TTCGTCTAAA TCCATCTGAT
    GCTGTTGTGG CCGTGGGGAA GCAACTTGAG ATAGAATGTT TGCCTCCAAA AGGTCATCCT
    GAGCCCAATG TTACTTGGAA GAAGGATGGG ATTCCAATAA ACCATAATAA TAGTCGATAC
    ACGATTTCTG CAGGCAAACT CTTTATTGGC AAAGCACTAA AGACGGATTC AGGACTATAT
    TCCTGCTTAG CATCAAATCA AGTGGGAGAA AGAGAGAGTA GAGCTGCTAA AATCGTTGTA
    CTGGAGAAGC CCATGTTTAC ATATAAACCC AGTGATGTGA CGGCAAAGTT TGGAAGCACC
    GTGCAGTTTG GTTGTGGAGC ACAAGGAGAC CCAAAGCCCA CCTTTCACTG GAGCAAAGAA
    AATGGAGACC TTCCACAAGG GAGGTATGAA ATTACCAATG AAAATACATT AAGAATCCGA
    TCTGTAACCT TGTATGACTC TGGAAAATAT ATCTGCACTG CAGGAAATCA GCTAGAAACA
    GTATCTGCCA AGGCATTGCT AACTGTTCAA GATCCTTTGG ATACTGGTCA GATAGAAAAG
    GAGAGACAAA TCTATAGAGA ATTAATGGGT GTTCGTGTTC ATTTAGACAA CATCACAGCA
    CTTCCAAGCG CATCAGTCTA TGTGCACTGG CAGGTCCTAA CGGAATCCCC CTTTACTGAA
    GGATATAGTG TATTTTACCG CACACTTTCT TCTACTGAAT CTGAGTGGAC AGAGTGGACA
    AAGTCTAGGA TCAATGAGTA CAGCACTGTT ATTCCATCTC TATGGAGGGG ACAGAAATAT
    GAGTTTAAGA TCCGACCTTT TGCTGGGAAA AATTATGGAC CTGACAGCAA TATTAAACAT
    CTCAGAATAC CTGAGGAAGT TCCAAATATT GCTCCACAGA ACATCAATGT GACTGCTGTG
    GAAGGAGGAA ACGGTACAAT AGTTGTGAGC TGGGAACCCC CACCAAAAAA CATTCACAGC
    AGTAACATAA AAGGTTATAA GGTCTGGTGT TTTGAGAATG AAACACATCC TAAAGCAGAC
    TGGATAGTGG ATGAAGAAAC CAAGAGTCTA GAAATTCCCA TGTTGGCCTC CGGCATTAAA
    TATCAAGTCC AAGTAGCGGC TATAAATGAT GCAGGAATTG GAATGCCAAG CATACCTAAA
    TACATAATGA TAGACTCACC AGAAGTGAAG GAGGTAAAAG ATGAAAATAT ATATTTTGAG
    CTAATCCTGC AAGTTGTGCG TCACCCAGCA TTCATTGGAA CTGTAGGAAC CGTGGCCTGG
    ATATTATTAA TGGTTGCAGC CGTGTATGTG TGTCAGCGTC ATTCTAAGCG CTATAGCACA
    AAACAACACA GTATATTAGG AAACAGTCTT TACCGGTTTG CGAGTGAAGA TACCATAATA
    AAGCACAGGA TGGACATTAG TGATTCCCCA TGGCTTTCAC ACACATGGAA ATCCGCCTCC
    TGCTCGCGAA ACTACAGCAG CACAATCAGT ATGAACAGCC AATTACTGTG GTCTGAAACT
    AAAGACACGG CAGATTTCCA TAAATCAACA ATGTCGTTTG AGCGCAAAAG TGAGGGTAGT
    CGAAGCCAGA TTATTCCGCT GGTGCCAGAC AGCAACAGCT TGTATGGAAC AATGTATGTC
    GACCTTCCAG GAAAAGATAT GACCACATTT CAGCGTCTGC CTTCTGACAA ATCTCAAGGC
    ATGGGGAGGC ATTTTAAAAC TCCTGACCCT CTAGGATTGC TTCATCACAG GCCATTGCCA
    TCATATTTGA ATGATTTCAG TGGCAGCAAT GATATGAATG GAGGAACTAG AAGCAAGTTG
    CCATGGCAAC CAAATATTAA CAATAATCCT GATATCTCAT TTAAGGACTC ATGGAGCAAA
    AACTGCAAGA AAGAGCTTCA ACATGCAAAT AGTGCTCCTT TGTCCCCTTA TTGCCAAGCA
    CCCAAACAGG GTGACAGTGT ACCAAGCATT CAGCATTTAA ACATCAAGAA AGTAGGAAAA
    GGGGACTATG CTAAAGTAAT GAAGACTTTC AGTTCCCCTA AAATCCTTCA CTACACCACC
    TCTCTCCAAG TGATGGATCT TCTACCTTAT ACACCTTCTC TCCCACCACC TCCAGTTCCT
    CCTCCAGATG AAAATCCAAT TCACAATGAC AAGGATAAAA TTGGTTGCAG TGTCTCATCA
    TATTCCGTGC GCAATCAAGA AAAGCAAGGC CATAAAATCA ACAGCAAGAA AAAGAGTCCC
    CCTGATACAC TGAACATTAA ACCAGCTTCA GAATGCTTCC CAATAAATGA TGATGGAGAT
    AATGTGTTAA CTCCAGAAGA TGTGGCACAA TATCTTGAAC TCAGTGATCA AGGGCAAAAT
    ACAAGGCATA AATCAGAATA TGATGCTTCC CTTCCAAGGC CTTTCTCTCC TTCTCAAACA
    TATGGCTACA TTTGCAGCCC GATACCTTCA GAAGCTGATG TTGTTGATGA AGATGATGAC
    CTTGATTTAG AGGACATCGG TTCATTAAAG TCTTATCGCA AGTACTGTGA AACTCCAACC
    TCCAGTATCA GTGAATATGA GAGCTCTATG GCCGGATCTC TTGTTAATGG CTGGGGTTCT
    GTTTCAGAAG ATAATTATAC CAGTGCTAGA TGCAGCATGG TCAGCTCCTC TGATGGGTCC
    TTTTTAATGG ATGCAAACTT CGCCAAGGCT CTTGCAGTTG CAGTTGACAG TTTTTGCTTT
    GGTGGATCTC AGACTGAGAC TGCTGGAAGA GATACGTTTT ATGCAGATCT TTCTCCGTCA
    GTGTCTCCTT TGGATGGAAT ATTAAACCAA CATAGTGGGG AAAATACAGA TGTTACCAAA
    AAGAAATCCA GAGTTAATCA TTTGCCTGTT TTAGACTGGA ACATTGACTG GATGGATGAA
    ATGGAAGCCA AGTACAGCAG GAAAAATGAC ATGAAACATC ATTTTCCTTT TGATAAAAAG
    ATGGATCCTT TCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62264
    Clone ID Related Accession (Same CDS sequence) XM_004916033.4
    Accession Version XM_004916033.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916033.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTTGCA GGAGGATTTT ATCCATCATT ATAATCATTT GGACAGAAGT ACATTGGTTG 
    GAAGGCTCAA AAACTCGATC AGAGGATTTT CCACCTCGTA TTATCGAACA CCCTTCAGAT
    TTGTTTGTGA AGAAGGACAA GCCAGCCACA TTGTATTGTC GAGCAGAGGG CAATCCACAG
    CCCACAATAG AATGGTACCG TAATGGAGAG CATGTGGAGA CCAGCACAGA TGATAAGACC
    CAGCGTTCCT ATGTTCAGTT GCCGGAGGGA CCACTCTTCT TCTTCAGCCT TAATCAACGA
    AAAGGCAAAT CTGATGAAGG GATTTATACT TGTGTGGCTA GAAACCATCT AGGAACGGCA
    TTTAGTAGAA ATGCTTCTCT TTATGTTGCT GCTCTAAGGG ATGATTTTCG TCTAAATCCA
    TCTGATGCTG TTGTGGCCGT GGGGAAGCAA CTTGAGATAG AATGTTTGCC TCCAAAAGGT
    CATCCTGAGC CCAATGTTAC TTGGAAGAAG GATGGGATTC CAATAAACCA TAATAATAGT
    CGATACACGA TTTCTGCAGG CAAACTCTTT ATTGGCAAAG CACTAAAGAC GGATTCAGGA
    CTATATTCCT GCTTAGCATC AAATCAAGTG GGAGAAAGAG AGAGTAGAGC TGCTAAAATC
    GTTGTACTGG AGAAGCCCAT GTTTACATAT AAACCCAGTG ATGTGACGGC AAAGTTTGGA
    AGCACCGTGC AGTTTGGTTG TGGAGCACAA GGAGACCCAA AGCCCACCTT TCACTGGAGC
    AAAGAAAATG GAGACCTTCC ACAAGGGAGG TATGAAATTA CCAATGAAAA TACATTAAGA
    ATCCGATCTG TAACCTTGTA TGACTCTGGA AAATATATCT GCACTGCAGG AAATCAGCTA
    GAAACAGTAT CTGCCAAGGC ATTGCTAACT GTTCAAGATC CTTTGGATAC TGGTCAGATA
    GAAAAGGAGA GACAAATCTA TAGAGAATTA ATGGGTGTTC GTGTTCATTT AGACAACATC
    ACAGCACTTC CAAGCGCATC AGTCTATGTG CACTGGCAGG TCCTAACGGA ATCCCCCTTT
    ACTGAAGGAT ATAGTGTATT TTACCGCACA CTTTCTTCTA CTGAATCTGA GTGGACAGAG
    TGGACAAAGT CTAGGATCAA TGAGTACAGC ACTGTTATTC CATCTCTATG GAGGGGACAG
    AAATATGAGT TTAAGATCCG ACCTTTTGCT GGGAAAAATT ATGGACCTGA CAGCAATATT
    AAACATCTCA GAATACCTGA GGAAGTTCCA AATATTGCTC CACAGAACAT CAATGTGACT
    GCTGTGGAAG GAGGAAACGG TACAATAGTT GTGAGCTGGG AACCCCCACC AAAAAACATT
    CACAGCAGTA ACATAAAAGG TTATAAGGTC TGGTGTTTTG AGAATGAAAC ACATCCTAAA
    GCAGACTGGA TAGTGGATGA AGAAACCAAG AGTCTAGAAA TTCCCATGTT GGCCTCCGGC
    ATTAAATATC AAGTCCAAGT AGCGGCTATA AATGATGCAG GAATTGGAAT GCCAAGCATA
    CCTAAATACA TAATGATAGA CTCACCAGAA GTGAAGGAGG TAAAAGATGA AAATATATAT
    TTTGAGCTAA TCCTGCAAGT TGTGCGTCAC CCAGCATTCA TTGGAACTGT AGGAACCGTG
    GCCTGGATAT TATTAATGGT TGCAGCCGTG TATGTGTGTC AGCGTCATTC TAAGCGCTAT
    AGCACAAAAC AACACAGTAT ATTAGGAAAC AGTCTTTACC GGTTTGCGAG TGAAGATACC
    ATAATAAAGC ACAGGATGGA CATTAGTGAT TCCCCATGGC TTTCACACAC ATGGAAATCC
    GCCTCCTGCT CGCGAAACTA CAGCAGCACA ATCAGTATGA ACAGCCAATT ACTGTGGTCT
    GAAACTAAAG ACACGGCAGA TTTCCATAAA TCAACAATGT CGTTTGAGCG CAAAAGTGAG
    GGTAGTCGAA GCCAGATTAT TCCGCTGGTG CCAGACAGCA ACAGCTTGTA TGGAACAATG
    TATGTCGACC TTCCAGGAAA AGATATGACC ACATTTCAGC GTCTGCCTTC TGACAAATCT
    CAAGGCATGG GGAGGCATTT TAAAACTCCT GACCCTCTAG GATTGCTTCA TCACAGGCCA
    TTGCCATCAT ATTTGAATGA TTTCAGTGGC AGCAATGATA TGAATGGAGG AACTAGAAGC
    AAGTTGCCAT GGCAACCAAA TATTAACAAT AATCCTGATA TCTCATTTAA GGACTCATGG
    AGCAAAAACT GCAAGAAAGA GCTTCAACAT GCAAATAGTG CTCCTTTGTC CCCTTATTGC
    CAAGCACCCA AACAGGGTGA CAGTGTACCA AGCATTCAGC ATTTAAACAT CAAGAAAGTA
    GGAAAAGGGG ACTATGCTAA AGTAATGAAG ACTTTCAGTT CCCCTAAAAT CCTTCACTAC
    ACCACCTCTC TCCAAGTGAT GGATCTTCTA CCTTATACAC CTTCTCTCCC ACCACCTCCA
    GTTCCTCCTC CAGATGAAAA TCCAATTCAC AATGACAAGG ATAAAATAAG TGGTTGCAGT
    GTCTCATCAT ATTCCGTGCG CAATCAAGAA AAGCAAGGCC ATAAAATCAA CAGCAAGAAA
    AAGAGTCCCC CTGATACACT GAACATTAAA CCAGCTTCAG AATGCTTCCC AATAAATGAT
    GATGGAGATA ATGTGTTAAC TCCAGAAGAT GTGGCACAAT ATCTTGAACT CAGTGATCAA
    GGGCAAAATA CAAGGCATAA ATCAGAATAT GATGCTTCCC TTCCAAGGCC TTTCTCTCCT
    TCTCAAACAT ATGGCTACAT TTGCAGCCCG ATACCTTCAG AAGCTGATGT TGTTGATGAA
    GATGATGACC TTGATTTAGA GGACATCGGT TCATTAAAGT CTTATCGCAA GTACTGTGAA
    ACTCCAACCT CCAGTATCAG TGAATATGAG AGCTCTATGG CCGGATCTCT TGTTAATGGC
    TGGGGTTCTG TTTCAGAAGA TAATTATACC AGTGCTAGAT GCAGCATGGT CAGCTCCTCT
    GATGGGTCCT TTTTAATGGA TGCAAACTTC GCCAAGGCTC TTGCAGTTGC AGTTGACAGT
    TTTTGCTTTG GTGGATCTCA GACTGAGACT GCTGGAAGAG ATACGTTTTA TGCAGATCTT
    TCTCCGTCAG TGTCTCCTTT GGATGGAATA TTAAACCAAC ATAGTGGGGA AAATACAGAT
    GTTACCAAAA AGAAATCCAG AGTTAATCAT TTGCCTGTTT TAGACTGGAA CATTGACTGG
    ATGGATGAAA TGGAAGCCAA GTACAGCAGG AAAAATGACA TGAAACATCA TTTTCCTTTT
    GATAAAAAGA TGGATCCTTT CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916090.2
    CDS245..3631
    Translation

    Target ORF information:

    RefSeq Version XM_004916033.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916033.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTTGCA GGAGGATTTT ATCCATCATT ATAATCATTT GGACAGAAGT ACATTGGTTG 
    GAAGGCTCAA AAACTCGATC AGAGGATTTT CCACCTCGTA TTATCGAACA CCCTTCAGAT
    TTGTTTGTGA AGAAGGACAA GCCAGCCACA TTGTATTGTC GAGCAGAGGG CAATCCACAG
    CCCACAATAG AATGGTACCG TAATGGAGAG CATGTGGAGA CCAGCACAGA TGATAAGACC
    CAGCGTTCCT ATGTTCAGTT GCCGGAGGGA CCACTCTTCT TCTTCAGCCT TAATCAACGA
    AAAGGCAAAT CTGATGAAGG GATTTATACT TGTGTGGCTA GAAACCATCT AGGAACGGCA
    TTTAGTAGAA ATGCTTCTCT TTATGTTGCT GCTCTAAGGG ATGATTTTCG TCTAAATCCA
    TCTGATGCTG TTGTGGCCGT GGGGAAGCAA CTTGAGATAG AATGTTTGCC TCCAAAAGGT
    CATCCTGAGC CCAATGTTAC TTGGAAGAAG GATGGGATTC CAATAAACCA TAATAATAGT
    CGATACACGA TTTCTGCAGG CAAACTCTTT ATTGGCAAAG CACTAAAGAC GGATTCAGGA
    CTATATTCCT GCTTAGCATC AAATCAAGTG GGAGAAAGAG AGAGTAGAGC TGCTAAAATC
    GTTGTACTGG AGAAGCCCAT GTTTACATAT AAACCCAGTG ATGTGACGGC AAAGTTTGGA
    AGCACCGTGC AGTTTGGTTG TGGAGCACAA GGAGACCCAA AGCCCACCTT TCACTGGAGC
    AAAGAAAATG GAGACCTTCC ACAAGGGAGG TATGAAATTA CCAATGAAAA TACATTAAGA
    ATCCGATCTG TAACCTTGTA TGACTCTGGA AAATATATCT GCACTGCAGG AAATCAGCTA
    GAAACAGTAT CTGCCAAGGC ATTGCTAACT GTTCAAGATC CTTTGGATAC TGGTCAGATA
    GAAAAGGAGA GACAAATCTA TAGAGAATTA ATGGGTGTTC GTGTTCATTT AGACAACATC
    ACAGCACTTC CAAGCGCATC AGTCTATGTG CACTGGCAGG TCCTAACGGA ATCCCCCTTT
    ACTGAAGGAT ATAGTGTATT TTACCGCACA CTTTCTTCTA CTGAATCTGA GTGGACAGAG
    TGGACAAAGT CTAGGATCAA TGAGTACAGC ACTGTTATTC CATCTCTATG GAGGGGACAG
    AAATATGAGT TTAAGATCCG ACCTTTTGCT GGGAAAAATT ATGGACCTGA CAGCAATATT
    AAACATCTCA GAATACCTGA GGAAGTTCCA AATATTGCTC CACAGAACAT CAATGTGACT
    GCTGTGGAAG GAGGAAACGG TACAATAGTT GTGAGCTGGG AACCCCCACC AAAAAACATT
    CACAGCAGTA ACATAAAAGG TTATAAGGTC TGGTGTTTTG AGAATGAAAC ACATCCTAAA
    GCAGACTGGA TAGTGGATGA AGAAACCAAG AGTCTAGAAA TTCCCATGTT GGCCTCCGGC
    ATTAAATATC AAGTCCAAGT AGCGGCTATA AATGATGCAG GAATTGGAAT GCCAAGCATA
    CCTAAATACA TAATGATAGA CTCACCAGAA GTGAAGGAGG TAAAAGATGA AAATATATAT
    TTTGAGCTAA TCCTGCAAGT TGTGCGTCAC CCAGCATTCA TTGGAACTGT AGGAACCGTG
    GCCTGGATAT TATTAATGGT TGCAGCCGTG TATGTGTGTC AGCGTCATTC TAAGCGCTAT
    AGCACAAAAC AACACAGTAT ATTAGGAAAC AGTCTTTACC GGTTTGCGAG TGAAGATACC
    ATAATAAAGC ACAGGATGGA CATTAGTGAT TCCCCATGGC TTTCACACAC ATGGAAATCC
    GCCTCCTGCT CGCGAAACTA CAGCAGCACA ATCAGTATGA ACAGCCAATT ACTGTGGTCT
    GAAACTAAAG ACACGGCAGA TTTCCATAAA TCAACAATGT CGTTTGAGCG CAAAAGTGAG
    GGTAGTCGAA GCCAGATTAT TCCGCTGGTG CCAGACAGCA ACAGCTTGTA TGGAACAATG
    TATGTCGACC TTCCAGGAAA AGATATGACC ACATTTCAGC GTCTGCCTTC TGACAAATCT
    CAAGGCATGG GGAGGCATTT TAAAACTCCT GACCCTCTAG GATTGCTTCA TCACAGGCCA
    TTGCCATCAT ATTTGAATGA TTTCAGTGGC AGCAATGATA TGAATGGAGG AACTAGAAGC
    AAGTTGCCAT GGCAACCAAA TATTAACAAT AATCCTGATA TCTCATTTAA GGACTCATGG
    AGCAAAAACT GCAAGAAAGA GCTTCAACAT GCAAATAGTG CTCCTTTGTC CCCTTATTGC
    CAAGCACCCA AACAGGGTGA CAGTGTACCA AGCATTCAGC ATTTAAACAT CAAGAAAGTA
    GGAAAAGGGG ACTATGCTAA AGTAATGAAG ACTTTCAGTT CCCCTAAAAT CCTTCACTAC
    ACCACCTCTC TCCAAGTGAT GGATCTTCTA CCTTATACAC CTTCTCTCCC ACCACCTCCA
    GTTCCTCCTC CAGATGAAAA TCCAATTCAC AATGACAAGG ATAAAATAAG TGGTTGCAGT
    GTCTCATCAT ATTCCGTGCG CAATCAAGAA AAGCAAGGCC ATAAAATCAA CAGCAAGAAA
    AAGAGTCCCC CTGATACACT GAACATTAAA CCAGCTTCAG AATGCTTCCC AATAAATGAT
    GATGGAGATA ATGTGTTAAC TCCAGAAGAT GTGGCACAAT ATCTTGAACT CAGTGATCAA
    GGGCAAAATA CAAGGCATAA ATCAGAATAT GATGCTTCCC TTCCAAGGCC TTTCTCTCCT
    TCTCAAACAT ATGGCTACAT TTGCAGCCCG ATACCTTCAG AAGCTGATGT TGTTGATGAA
    GATGATGACC TTGATTTAGA GGACATCGGT TCATTAAAGT CTTATCGCAA GTACTGTGAA
    ACTCCAACCT CCAGTATCAG TGAATATGAG AGCTCTATGG CCGGATCTCT TGTTAATGGC
    TGGGGTTCTG TTTCAGAAGA TAATTATACC AGTGCTAGAT GCAGCATGGT CAGCTCCTCT
    GATGGGTCCT TTTTAATGGA TGCAAACTTC GCCAAGGCTC TTGCAGTTGC AGTTGACAGT
    TTTTGCTTTG GTGGATCTCA GACTGAGACT GCTGGAAGAG ATACGTTTTA TGCAGATCTT
    TCTCCGTCAG TGTCTCCTTT GGATGGAATA TTAAACCAAC ATAGTGGGGA AAATACAGAT
    GTTACCAAAA AGAAATCCAG AGTTAATCAT TTGCCTGTTT TAGACTGGAA CATTGACTGG
    ATGGATGAAA TGGAAGCCAA GTACAGCAGG AAAAATGACA TGAAACATCA TTTTCCTTTT
    GATAAAAAGA TGGATCCTTT CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa13010
    Clone ID Related Accession (Same CDS sequence) XM_002937520.3
    Accession Version XM_002937520.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002937520.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAATTTTG CCTACTTTCT TCCAATTTTT GTACTTTTTC CCTTGGGATG GTGTTCACAG 
    GTGAGCTCCT GCCAATGTCA TCAGCCAGTC ATTAATAAAG AACATGGCCT GGAAGCTACA
    CGTAGACAGA CCCGGCATGT TCAGCACCAC CGGTCAACAA GAAGGCACCG TATTAGAGGC
    TCAAAAACTC GATCAGAGGA TTTTCCACCT CGTATTATCG AACACCCTTC AGATTTGTTT
    GTGAAGAAGG ACAAGCCAGC CACATTGTAT TGTCGAGCAG AGGGCAATCC ACAGCCCACA
    ATAGAATGGT ACCGTAATGG AGAGCATGTG GAGACCAGCA CAGATGATAA GACCCAGCGT
    TCCTATGTTC AGTTGCCGGA GGGACCACTC TTCTTCTTCA GCCTTAATCA ACGAAAAGGC
    AAATCTGATG AAGGGATTTA TACTTGTGTG GCTAGAAACC ATCTAGGAAC GGCATTTAGT
    AGAAATGCTT CTCTTTATGT TGCTGCTCTA AGGGATGATT TTCGTCTAAA TCCATCTGAT
    GCTGTTGTGG CCGTGGGGAA GCAACTTGAG ATAGAATGTT TGCCTCCAAA AGGTCATCCT
    GAGCCCAATG TTACTTGGAA GAAGGATGGG ATTCCAATAA ACCATAATAA TAGTCGATAC
    ACGATTTCTG CAGGCAAACT CTTTATTGGC AAAGCACTAA AGACGGATTC AGGACTATAT
    TCCTGCTTAG CATCAAATCA AGTGGGAGAA AGAGAGAGTA GAGCTGCTAA AATCGTTGTA
    CTGGAGAAGC CCATGTTTAC ATATAAACCC AGTGATGTGA CGGCAAAGTT TGGAAGCACC
    GTGCAGTTTG GTTGTGGAGC ACAAGGAGAC CCAAAGCCCA CCTTTCACTG GAGCAAAGAA
    AATGGAGACC TTCCACAAGG GAGGTATGAA ATTACCAATG AAAATACATT AAGAATCCGA
    TCTGTAACCT TGTATGACTC TGGAAAATAT ATCTGCACTG CAGGAAATCA GCTAGAAACA
    GTATCTGCCA AGGCATTGCT AACTGTTCAA GATCCTTTGG ATACTGGTCA GATAGAAAAG
    GAGAGACAAA TCTATAGAGA ATTAATGGGT GTTCGTGTTC ATTTAGACAA CATCACAGCA
    CTTCCAAGCG CATCAGTCTA TGTGCACTGG CAGGTCCTAA CGGAATCCCC CTTTACTGAA
    GGATATAGTG TATTTTACCG CACACTTTCT TCTACTGAAT CTGAGTGGAC AGAGTGGACA
    AAGTCTAGGA TCAATGAGTA CAGCACTGTT ATTCCATCTC TATGGAGGGG ACAGAAATAT
    GAGTTTAAGA TCCGACCTTT TGCTGGGAAA AATTATGGAC CTGACAGCAA TATTAAACAT
    CTCAGAATAC CTGAGGAAGT TCCAAATATT GCTCCACAGA ACATCAATGT GACTGCTGTG
    GAAGGAGGAA ACGGTACAAT AGTTGTGAGC TGGGAACCCC CACCAAAAAA CATTCACAGC
    AGTAACATAA AAGGTTATAA GGTCTGGTGT TTTGAGAATG AAACACATCC TAAAGCAGAC
    TGGATAGTGG ATGAAGAAAC CAAGAGTCTA GAAATTCCCA TGTTGGCCTC CGGCATTAAA
    TATCAAGTCC AAGTAGCGGC TATAAATGAT GCAGGAATTG GAATGCCAAG CATACCTAAA
    TACATAATGA TAGACTCACC AGAAGTGAAG GAGGTAAAAG ATGAAAATAT ATATTTTGAG
    CTAATCCTGC AAGTTGTGCG TCACCCAGCA TTCATTGGAA CTGTAGGAAC CGTGGCCTGG
    ATATTATTAA TGGTTGCAGC CGTGTATGTG TGTCAGCGTC ATTCTAAGCG CTATAGCACA
    AAACAACACA GTATATTAGG AAACAGTCTT TACCGGTTTG CGAGTGAAGA TACCATAATA
    AAGCACAGGA TGGACATTAG TGATTCCCCA TGGCTTTCAC ACACATGGAA ATCCGCCTCC
    TGCTCGCGAA ACTACAGCAG CACAATCAGT ATGAACAGCC AATTACTGTG GTCTGAAACT
    AAAGACACGG CAGATTTCCA TAAATCAACA ATGTCGTTTG AGCGCAAAAG TGAGGGTAGT
    CGAAGCCAGA TTATTCCGCT GGTGCCAGAC AGCAACAGCT TGTATGGAAC AATGTATGTC
    GACCTTCCAG GAAAAGATAT GACCACATTT CAGCGTCTGC CTTCTGACAA ATCTCAAGGC
    ATGGGGAGAC ATTTTAAAAC TCCTGACCCT CTAGGATTGC TTCATCACAG GCCATTGCCA
    TCATATTTGA ATGATTTCAG TGGCAGCAAT GATATGAATG GAGGAACTAG AAGCAAGTTG
    CCATGGCAAC CAAATATTAA CAATAATCCT GATATCTCAT TTAAGGACTC ATGGAGCAAA
    AACTGCAAGA AAGAGCTTCA ACATGCAAAT AGTGCTCCTT TGTCCCCTTA TTGCCAAGCA
    CCCAAACAGG GTGACAGTGT ACCAAGCATT CAGCATTTAA ACATCAAGAA AGTAGGAAAA
    GGGGACTATG CTAAAGTAAT GAAGACTTTC AGTTCCCCTA AAATCCTTCA CTACACCACC
    TCTCTCCAAG TGATGGATCT TCTACCCTAT ACACCTTCTC TCCCACCACC TCCAGTTCCT
    CCTCCAGATG AAAATCCAAT TCACAATGAC AAGGATAAAA TAAGTGGTTG CAGTGTCTCA
    TCATATTCCG TGCGCAATCA AGAAAAGCAA GGCCATAAAA TCAACAGCAA GAAAAAGAGT
    CCCCCTGATA CACTGAACAT TAAACCAGCT TCAGAATGCT TCCCAATAAA TGATGATGGA
    GATAATGTGT TAACTCCAGA AGATGTGGCA CAATATCTTG AACTCAGTGA TCAAGGGCAA
    AATACAAGGC ATAAATCAGA ATATGATGCT TCCCTTCCAA GGCCTTTCTC TCCTTCTCAA
    ACATATGGCT ACATTTGCAG CCCGATACCT TCAGAAGCTG ATGTTGTTGA TGAAGATGAT
    GACCTTGATT TAGAGGACAT CGGTTCATTA AAGTCTTATC GCAAGTACTG TGAAACTCCA
    ACCTCCAGTA TCAGTGAATA TGAGAGCTCT ATGGCCGGAT CTCTTGTTAA TGGCTGGGGT
    TCTGTTTCAG AAGATAATTA TACCAGTGCT AGATGCAGCA TGGTCAGCTC CTCTGATGGG
    TCCTTTTTAA TGGATGCAAA CTTCGCCAAG GCTCTTGCAG TTGCAGTTGA CAGTTTTTGC
    TTTGGTGGAT CTCAGACTGA GACTGCTGGA AGAGATACGT TTTATGCAGA TCTTTCTCCG
    TCAGTGTCTC CTTTGGATGG AATATTAAAC CAACAAAGTG GGGAAAATAC AGATGTTACC
    AAAAAGAAAT CCAGAGTTAA TCATTTGCCT GTTTTAGACT GGAACATTGA CTGGATGGAT
    GAAATGGAAG CCAGGTACAG CAGGAAAAAT GACATGAAAC ATCATTTTCC TTTTGATAAA
    AAGATGGATC CTTTCAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937566.2
    CDS125..3625
    Translation

    Target ORF information:

    RefSeq Version XM_002937520.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937520.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAATTTTG CCTACTTTCT TCCAATTTTT GTACTTTTTC CCTTGGGATG GTGTTCACAG 
    GTGAGCTCCT GCCAATGTCA TCAGCCAGTC ATTAATAAAG AACATGGCCT GGAAGCTACA
    CGTAGACAGA CCCGGCATGT TCAGCACCAC CGGTCAACAA GAAGGCACCG TATTAGAGGC
    TCAAAAACTC GATCAGAGGA TTTTCCACCT CGTATTATCG AACACCCTTC AGATTTGTTT
    GTGAAGAAGG ACAAGCCAGC CACATTGTAT TGTCGAGCAG AGGGCAATCC ACAGCCCACA
    ATAGAATGGT ACCGTAATGG AGAGCATGTG GAGACCAGCA CAGATGATAA GACCCAGCGT
    TCCTATGTTC AGTTGCCGGA GGGACCACTC TTCTTCTTCA GCCTTAATCA ACGAAAAGGC
    AAATCTGATG AAGGGATTTA TACTTGTGTG GCTAGAAACC ATCTAGGAAC GGCATTTAGT
    AGAAATGCTT CTCTTTATGT TGCTGCTCTA AGGGATGATT TTCGTCTAAA TCCATCTGAT
    GCTGTTGTGG CCGTGGGGAA GCAACTTGAG ATAGAATGTT TGCCTCCAAA AGGTCATCCT
    GAGCCCAATG TTACTTGGAA GAAGGATGGG ATTCCAATAA ACCATAATAA TAGTCGATAC
    ACGATTTCTG CAGGCAAACT CTTTATTGGC AAAGCACTAA AGACGGATTC AGGACTATAT
    TCCTGCTTAG CATCAAATCA AGTGGGAGAA AGAGAGAGTA GAGCTGCTAA AATCGTTGTA
    CTGGAGAAGC CCATGTTTAC ATATAAACCC AGTGATGTGA CGGCAAAGTT TGGAAGCACC
    GTGCAGTTTG GTTGTGGAGC ACAAGGAGAC CCAAAGCCCA CCTTTCACTG GAGCAAAGAA
    AATGGAGACC TTCCACAAGG GAGGTATGAA ATTACCAATG AAAATACATT AAGAATCCGA
    TCTGTAACCT TGTATGACTC TGGAAAATAT ATCTGCACTG CAGGAAATCA GCTAGAAACA
    GTATCTGCCA AGGCATTGCT AACTGTTCAA GATCCTTTGG ATACTGGTCA GATAGAAAAG
    GAGAGACAAA TCTATAGAGA ATTAATGGGT GTTCGTGTTC ATTTAGACAA CATCACAGCA
    CTTCCAAGCG CATCAGTCTA TGTGCACTGG CAGGTCCTAA CGGAATCCCC CTTTACTGAA
    GGATATAGTG TATTTTACCG CACACTTTCT TCTACTGAAT CTGAGTGGAC AGAGTGGACA
    AAGTCTAGGA TCAATGAGTA CAGCACTGTT ATTCCATCTC TATGGAGGGG ACAGAAATAT
    GAGTTTAAGA TCCGACCTTT TGCTGGGAAA AATTATGGAC CTGACAGCAA TATTAAACAT
    CTCAGAATAC CTGAGGAAGT TCCAAATATT GCTCCACAGA ACATCAATGT GACTGCTGTG
    GAAGGAGGAA ACGGTACAAT AGTTGTGAGC TGGGAACCCC CACCAAAAAA CATTCACAGC
    AGTAACATAA AAGGTTATAA GGTCTGGTGT TTTGAGAATG AAACACATCC TAAAGCAGAC
    TGGATAGTGG ATGAAGAAAC CAAGAGTCTA GAAATTCCCA TGTTGGCCTC CGGCATTAAA
    TATCAAGTCC AAGTAGCGGC TATAAATGAT GCAGGAATTG GAATGCCAAG CATACCTAAA
    TACATAATGA TAGACTCACC AGAAGTGAAG GAGGTAAAAG ATGAAAATAT ATATTTTGAG
    CTAATCCTGC AAGTTGTGCG TCACCCAGCA TTCATTGGAA CTGTAGGAAC CGTGGCCTGG
    ATATTATTAA TGGTTGCAGC CGTGTATGTG TGTCAGCGTC ATTCTAAGCG CTATAGCACA
    AAACAACACA GTATATTAGG AAACAGTCTT TACCGGTTTG CGAGTGAAGA TACCATAATA
    AAGCACAGGA TGGACATTAG TGATTCCCCA TGGCTTTCAC ACACATGGAA ATCCGCCTCC
    TGCTCGCGAA ACTACAGCAG CACAATCAGT ATGAACAGCC AATTACTGTG GTCTGAAACT
    AAAGACACGG CAGATTTCCA TAAATCAACA ATGTCGTTTG AGCGCAAAAG TGAGGGTAGT
    CGAAGCCAGA TTATTCCGCT GGTGCCAGAC AGCAACAGCT TGTATGGAAC AATGTATGTC
    GACCTTCCAG GAAAAGATAT GACCACATTT CAGCGTCTGC CTTCTGACAA ATCTCAAGGC
    ATGGGGAGAC ATTTTAAAAC TCCTGACCCT CTAGGATTGC TTCATCACAG GCCATTGCCA
    TCATATTTGA ATGATTTCAG TGGCAGCAAT GATATGAATG GAGGAACTAG AAGCAAGTTG
    CCATGGCAAC CAAATATTAA CAATAATCCT GATATCTCAT TTAAGGACTC ATGGAGCAAA
    AACTGCAAGA AAGAGCTTCA ACATGCAAAT AGTGCTCCTT TGTCCCCTTA TTGCCAAGCA
    CCCAAACAGG GTGACAGTGT ACCAAGCATT CAGCATTTAA ACATCAAGAA AGTAGGAAAA
    GGGGACTATG CTAAAGTAAT GAAGACTTTC AGTTCCCCTA AAATCCTTCA CTACACCACC
    TCTCTCCAAG TGATGGATCT TCTACCCTAT ACACCTTCTC TCCCACCACC TCCAGTTCCT
    CCTCCAGATG AAAATCCAAT TCACAATGAC AAGGATAAAA TAAGTGGTTG CAGTGTCTCA
    TCATATTCCG TGCGCAATCA AGAAAAGCAA GGCCATAAAA TCAACAGCAA GAAAAAGAGT
    CCCCCTGATA CACTGAACAT TAAACCAGCT TCAGAATGCT TCCCAATAAA TGATGATGGA
    GATAATGTGT TAACTCCAGA AGATGTGGCA CAATATCTTG AACTCAGTGA TCAAGGGCAA
    AATACAAGGC ATAAATCAGA ATATGATGCT TCCCTTCCAA GGCCTTTCTC TCCTTCTCAA
    ACATATGGCT ACATTTGCAG CCCGATACCT TCAGAAGCTG ATGTTGTTGA TGAAGATGAT
    GACCTTGATT TAGAGGACAT CGGTTCATTA AAGTCTTATC GCAAGTACTG TGAAACTCCA
    ACCTCCAGTA TCAGTGAATA TGAGAGCTCT ATGGCCGGAT CTCTTGTTAA TGGCTGGGGT
    TCTGTTTCAG AAGATAATTA TACCAGTGCT AGATGCAGCA TGGTCAGCTC CTCTGATGGG
    TCCTTTTTAA TGGATGCAAA CTTCGCCAAG GCTCTTGCAG TTGCAGTTGA CAGTTTTTGC
    TTTGGTGGAT CTCAGACTGA GACTGCTGGA AGAGATACGT TTTATGCAGA TCTTTCTCCG
    TCAGTGTCTC CTTTGGATGG AATATTAAAC CAACAAAGTG GGGAAAATAC AGATGTTACC
    AAAAAGAAAT CCAGAGTTAA TCATTTGCCT GTTTTAGACT GGAACATTGA CTGGATGGAT
    GAAATGGAAG CCAGGTACAG CAGGAAAAAT GACATGAAAC ATCATTTTCC TTTTGATAAA
    AAGATGGATC CTTTCAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa13011
    Clone ID Related Accession (Same CDS sequence) XM_004916033.3
    Accession Version XM_004916033.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916033.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTTGCA GGAGGATTTT ATCCATCATT ATAATCATTT GGACAGAAGT ACATTGGTTG 
    GAAGGCTCAA AAACTCGATC AGAGGATTTT CCACCTCGTA TTATCGAACA CCCTTCAGAT
    TTGTTTGTGA AGAAGGACAA GCCAGCCACA TTGTATTGTC GAGCAGAGGG CAATCCACAG
    CCCACAATAG AATGGTACCG TAATGGAGAG CATGTGGAGA CCAGCACAGA TGATAAGACC
    CAGCGTTCCT ATGTTCAGTT GCCGGAGGGA CCACTCTTCT TCTTCAGCCT TAATCAACGA
    AAAGGCAAAT CTGATGAAGG GATTTATACT TGTGTGGCTA GAAACCATCT AGGAACGGCA
    TTTAGTAGAA ATGCTTCTCT TTATGTTGCT GCTCTAAGGG ATGATTTTCG TCTAAATCCA
    TCTGATGCTG TTGTGGCCGT GGGGAAGCAA CTTGAGATAG AATGTTTGCC TCCAAAAGGT
    CATCCTGAGC CCAATGTTAC TTGGAAGAAG GATGGGATTC CAATAAACCA TAATAATAGT
    CGATACACGA TTTCTGCAGG CAAACTCTTT ATTGGCAAAG CACTAAAGAC GGATTCAGGA
    CTATATTCCT GCTTAGCATC AAATCAAGTG GGAGAAAGAG AGAGTAGAGC TGCTAAAATC
    GTTGTACTGG AGAAGCCCAT GTTTACATAT AAACCCAGTG ATGTGACGGC AAAGTTTGGA
    AGCACCGTGC AGTTTGGTTG TGGAGCACAA GGAGACCCAA AGCCCACCTT TCACTGGAGC
    AAAGAAAATG GAGACCTTCC ACAAGGGAGG TATGAAATTA CCAATGAAAA TACATTAAGA
    ATCCGATCTG TAACCTTGTA TGACTCTGGA AAATATATCT GCACTGCAGG AAATCAGCTA
    GAAACAGTAT CTGCCAAGGC ATTGCTAACT GTTCAAGATC CTTTGGATAC TGGTCAGATA
    GAAAAGGAGA GACAAATCTA TAGAGAATTA ATGGGTGTTC GTGTTCATTT AGACAACATC
    ACAGCACTTC CAAGCGCATC AGTCTATGTG CACTGGCAGG TCCTAACGGA ATCCCCCTTT
    ACTGAAGGAT ATAGTGTATT TTACCGCACA CTTTCTTCTA CTGAATCTGA GTGGACAGAG
    TGGACAAAGT CTAGGATCAA TGAGTACAGC ACTGTTATTC CATCTCTATG GAGGGGACAG
    AAATATGAGT TTAAGATCCG ACCTTTTGCT GGGAAAAATT ATGGACCTGA CAGCAATATT
    AAACATCTCA GAATACCTGA GGAAGTTCCA AATATTGCTC CACAGAACAT CAATGTGACT
    GCTGTGGAAG GAGGAAACGG TACAATAGTT GTGAGCTGGG AACCCCCACC AAAAAACATT
    CACAGCAGTA ACATAAAAGG TTATAAGGTC TGGTGTTTTG AGAATGAAAC ACATCCTAAA
    GCAGACTGGA TAGTGGATGA AGAAACCAAG AGTCTAGAAA TTCCCATGTT GGCCTCCGGC
    ATTAAATATC AAGTCCAAGT AGCGGCTATA AATGATGCAG GAATTGGAAT GCCAAGCATA
    CCTAAATACA TAATGATAGA CTCACCAGAA GTGAAGGAGG TAAAAGATGA AAATATATAT
    TTTGAGCTAA TCCTGCAAGT TGTGCGTCAC CCAGCATTCA TTGGAACTGT AGGAACCGTG
    GCCTGGATAT TATTAATGGT TGCAGCCGTG TATGTGTGTC AGCGTCATTC TAAGCGCTAT
    AGCACAAAAC AACACAGTAT ATTAGGAAAC AGTCTTTACC GGTTTGCGAG TGAAGATACC
    ATAATAAAGC ACAGGATGGA CATTAGTGAT TCCCCATGGC TTTCACACAC ATGGAAATCC
    GCCTCCTGCT CGCGAAACTA CAGCAGCACA ATCAGTATGA ACAGCCAATT ACTGTGGTCT
    GAAACTAAAG ACACGGCAGA TTTCCATAAA TCAACAATGT CGTTTGAGCG CAAAAGTGAG
    GGTAGTCGAA GCCAGATTAT TCCGCTGGTG CCAGACAGCA ACAGCTTGTA TGGAACAATG
    TATGTCGACC TTCCAGGAAA AGATATGACC ACATTTCAGC GTCTGCCTTC TGACAAATCT
    CAAGGCATGG GGAGACATTT TAAAACTCCT GACCCTCTAG GATTGCTTCA TCACAGGCCA
    TTGCCATCAT ATTTGAATGA TTTCAGTGGC AGCAATGATA TGAATGGAGG AACTAGAAGC
    AAGTTGCCAT GGCAACCAAA TATTAACAAT AATCCTGATA TCTCATTTAA GGACTCATGG
    AGCAAAAACT GCAAGAAAGA GCTTCAACAT GCAAATAGTG CTCCTTTGTC CCCTTATTGC
    CAAGCACCCA AACAGGGTGA CAGTGTACCA AGCATTCAGC ATTTAAACAT CAAGAAAGTA
    GGAAAAGGGG ACTATGCTAA AGTAATGAAG ACTTTCAGTT CCCCTAAAAT CCTTCACTAC
    ACCACCTCTC TCCAAGTGAT GGATCTTCTA CCCTATACAC CTTCTCTCCC ACCACCTCCA
    GTTCCTCCTC CAGATGAAAA TCCAATTCAC AATGACAAGG ATAAAATAAG TGGTTGCAGT
    GTCTCATCAT ATTCCGTGCG CAATCAAGAA AAGCAAGGCC ATAAAATCAA CAGCAAGAAA
    AAGAGTCCCC CTGATACACT GAACATTAAA CCAGCTTCAG AATGCTTCCC AATAAATGAT
    GATGGAGATA ATGTGTTAAC TCCAGAAGAT GTGGCACAAT ATCTTGAACT CAGTGATCAA
    GGGCAAAATA CAAGGCATAA ATCAGAATAT GATGCTTCCC TTCCAAGGCC TTTCTCTCCT
    TCTCAAACAT ATGGCTACAT TTGCAGCCCG ATACCTTCAG AAGCTGATGT TGTTGATGAA
    GATGATGACC TTGATTTAGA GGACATCGGT TCATTAAAGT CTTATCGCAA GTACTGTGAA
    ACTCCAACCT CCAGTATCAG TGAATATGAG AGCTCTATGG CCGGATCTCT TGTTAATGGC
    TGGGGTTCTG TTTCAGAAGA TAATTATACC AGTGCTAGAT GCAGCATGGT CAGCTCCTCT
    GATGGGTCCT TTTTAATGGA TGCAAACTTC GCCAAGGCTC TTGCAGTTGC AGTTGACAGT
    TTTTGCTTTG GTGGATCTCA GACTGAGACT GCTGGAAGAG ATACGTTTTA TGCAGATCTT
    TCTCCGTCAG TGTCTCCTTT GGATGGAATA TTAAACCAAC AAAGTGGGGA AAATACAGAT
    GTTACCAAAA AGAAATCCAG AGTTAATCAT TTGCCTGTTT TAGACTGGAA CATTGACTGG
    ATGGATGAAA TGGAAGCCAG GTACAGCAGG AAAAATGACA TGAAACATCA TTTTCCTTTT
    GATAAAAAGA TGGATCCTTT CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916090.1
    CDS246..3632
    Translation

    Target ORF information:

    RefSeq Version XM_004916033.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916033.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGTTTGCA GGAGGATTTT ATCCATCATT ATAATCATTT GGACAGAAGT ACATTGGTTG 
    GAAGGCTCAA AAACTCGATC AGAGGATTTT CCACCTCGTA TTATCGAACA CCCTTCAGAT
    TTGTTTGTGA AGAAGGACAA GCCAGCCACA TTGTATTGTC GAGCAGAGGG CAATCCACAG
    CCCACAATAG AATGGTACCG TAATGGAGAG CATGTGGAGA CCAGCACAGA TGATAAGACC
    CAGCGTTCCT ATGTTCAGTT GCCGGAGGGA CCACTCTTCT TCTTCAGCCT TAATCAACGA
    AAAGGCAAAT CTGATGAAGG GATTTATACT TGTGTGGCTA GAAACCATCT AGGAACGGCA
    TTTAGTAGAA ATGCTTCTCT TTATGTTGCT GCTCTAAGGG ATGATTTTCG TCTAAATCCA
    TCTGATGCTG TTGTGGCCGT GGGGAAGCAA CTTGAGATAG AATGTTTGCC TCCAAAAGGT
    CATCCTGAGC CCAATGTTAC TTGGAAGAAG GATGGGATTC CAATAAACCA TAATAATAGT
    CGATACACGA TTTCTGCAGG CAAACTCTTT ATTGGCAAAG CACTAAAGAC GGATTCAGGA
    CTATATTCCT GCTTAGCATC AAATCAAGTG GGAGAAAGAG AGAGTAGAGC TGCTAAAATC
    GTTGTACTGG AGAAGCCCAT GTTTACATAT AAACCCAGTG ATGTGACGGC AAAGTTTGGA
    AGCACCGTGC AGTTTGGTTG TGGAGCACAA GGAGACCCAA AGCCCACCTT TCACTGGAGC
    AAAGAAAATG GAGACCTTCC ACAAGGGAGG TATGAAATTA CCAATGAAAA TACATTAAGA
    ATCCGATCTG TAACCTTGTA TGACTCTGGA AAATATATCT GCACTGCAGG AAATCAGCTA
    GAAACAGTAT CTGCCAAGGC ATTGCTAACT GTTCAAGATC CTTTGGATAC TGGTCAGATA
    GAAAAGGAGA GACAAATCTA TAGAGAATTA ATGGGTGTTC GTGTTCATTT AGACAACATC
    ACAGCACTTC CAAGCGCATC AGTCTATGTG CACTGGCAGG TCCTAACGGA ATCCCCCTTT
    ACTGAAGGAT ATAGTGTATT TTACCGCACA CTTTCTTCTA CTGAATCTGA GTGGACAGAG
    TGGACAAAGT CTAGGATCAA TGAGTACAGC ACTGTTATTC CATCTCTATG GAGGGGACAG
    AAATATGAGT TTAAGATCCG ACCTTTTGCT GGGAAAAATT ATGGACCTGA CAGCAATATT
    AAACATCTCA GAATACCTGA GGAAGTTCCA AATATTGCTC CACAGAACAT CAATGTGACT
    GCTGTGGAAG GAGGAAACGG TACAATAGTT GTGAGCTGGG AACCCCCACC AAAAAACATT
    CACAGCAGTA ACATAAAAGG TTATAAGGTC TGGTGTTTTG AGAATGAAAC ACATCCTAAA
    GCAGACTGGA TAGTGGATGA AGAAACCAAG AGTCTAGAAA TTCCCATGTT GGCCTCCGGC
    ATTAAATATC AAGTCCAAGT AGCGGCTATA AATGATGCAG GAATTGGAAT GCCAAGCATA
    CCTAAATACA TAATGATAGA CTCACCAGAA GTGAAGGAGG TAAAAGATGA AAATATATAT
    TTTGAGCTAA TCCTGCAAGT TGTGCGTCAC CCAGCATTCA TTGGAACTGT AGGAACCGTG
    GCCTGGATAT TATTAATGGT TGCAGCCGTG TATGTGTGTC AGCGTCATTC TAAGCGCTAT
    AGCACAAAAC AACACAGTAT ATTAGGAAAC AGTCTTTACC GGTTTGCGAG TGAAGATACC
    ATAATAAAGC ACAGGATGGA CATTAGTGAT TCCCCATGGC TTTCACACAC ATGGAAATCC
    GCCTCCTGCT CGCGAAACTA CAGCAGCACA ATCAGTATGA ACAGCCAATT ACTGTGGTCT
    GAAACTAAAG ACACGGCAGA TTTCCATAAA TCAACAATGT CGTTTGAGCG CAAAAGTGAG
    GGTAGTCGAA GCCAGATTAT TCCGCTGGTG CCAGACAGCA ACAGCTTGTA TGGAACAATG
    TATGTCGACC TTCCAGGAAA AGATATGACC ACATTTCAGC GTCTGCCTTC TGACAAATCT
    CAAGGCATGG GGAGACATTT TAAAACTCCT GACCCTCTAG GATTGCTTCA TCACAGGCCA
    TTGCCATCAT ATTTGAATGA TTTCAGTGGC AGCAATGATA TGAATGGAGG AACTAGAAGC
    AAGTTGCCAT GGCAACCAAA TATTAACAAT AATCCTGATA TCTCATTTAA GGACTCATGG
    AGCAAAAACT GCAAGAAAGA GCTTCAACAT GCAAATAGTG CTCCTTTGTC CCCTTATTGC
    CAAGCACCCA AACAGGGTGA CAGTGTACCA AGCATTCAGC ATTTAAACAT CAAGAAAGTA
    GGAAAAGGGG ACTATGCTAA AGTAATGAAG ACTTTCAGTT CCCCTAAAAT CCTTCACTAC
    ACCACCTCTC TCCAAGTGAT GGATCTTCTA CCCTATACAC CTTCTCTCCC ACCACCTCCA
    GTTCCTCCTC CAGATGAAAA TCCAATTCAC AATGACAAGG ATAAAATAAG TGGTTGCAGT
    GTCTCATCAT ATTCCGTGCG CAATCAAGAA AAGCAAGGCC ATAAAATCAA CAGCAAGAAA
    AAGAGTCCCC CTGATACACT GAACATTAAA CCAGCTTCAG AATGCTTCCC AATAAATGAT
    GATGGAGATA ATGTGTTAAC TCCAGAAGAT GTGGCACAAT ATCTTGAACT CAGTGATCAA
    GGGCAAAATA CAAGGCATAA ATCAGAATAT GATGCTTCCC TTCCAAGGCC TTTCTCTCCT
    TCTCAAACAT ATGGCTACAT TTGCAGCCCG ATACCTTCAG AAGCTGATGT TGTTGATGAA
    GATGATGACC TTGATTTAGA GGACATCGGT TCATTAAAGT CTTATCGCAA GTACTGTGAA
    ACTCCAACCT CCAGTATCAG TGAATATGAG AGCTCTATGG CCGGATCTCT TGTTAATGGC
    TGGGGTTCTG TTTCAGAAGA TAATTATACC AGTGCTAGAT GCAGCATGGT CAGCTCCTCT
    GATGGGTCCT TTTTAATGGA TGCAAACTTC GCCAAGGCTC TTGCAGTTGC AGTTGACAGT
    TTTTGCTTTG GTGGATCTCA GACTGAGACT GCTGGAAGAG ATACGTTTTA TGCAGATCTT
    TCTCCGTCAG TGTCTCCTTT GGATGGAATA TTAAACCAAC AAAGTGGGGA AAATACAGAT
    GTTACCAAAA AGAAATCCAG AGTTAATCAT TTGCCTGTTT TAGACTGGAA CATTGACTGG
    ATGGATGAAA TGGAAGCCAG GTACAGCAGG AAAAATGACA TGAAACATCA TTTTCCTTTT
    GATAAAAAGA TGGATCCTTT CAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35654
    Clone ID Related Accession (Same CDS sequence) XM_012966984.1
    Accession Version XM_012966984.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product roundabout homolog 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAATTTTG CCTACTTTCT TCCAATTTTT GTACTTTTTC CCTTGGGATG GTGTTCACAG 
    GTGAGCTCCT GCCAATGTCA TCAGCCAGTC ATTAATAAAG AACATGGCCT GGAAGCTACA
    CGTAGACAGA CCCGGCATGT TCAGCACCAC CGGTCAACAA GAAGGCACCG TATTAGAGGC
    TCAAAAACTC GATCAGAGGA TTTTCCACCT CGTATTATCG AACACCCTTC AGATTTGTTT
    GTGAAGAAGG ACAAGCCAGC CACATTGTAT TGTCGAGCAG AGGGCAATCC ACAGCCCACA
    ATAGAATGGT ACCGTAATGG AGAGCATGTG GAGACCAGCA CAGATGATAA GACCCAGCGT
    TCCTATGTTC AGTTGCCGGA GGGACCACTC TTCTTCTTCA GCCTTAATCA ACGAAAAGGC
    AAATCTGATG AAGGGATTTA TACTTGTGTG GCTAGAAACC ATCTAGGAAC GGCATTTAGT
    AGAAATGCTT CTCTTTATGT TGCTGCTCTA AGGGATGATT TTCGTCTAAA TCCATCTGAT
    GCTGTTGTGG CCGTGGGGAA GCAACTTGAG ATAGAATGTT TGCCTCCAAA AGGTCATCCT
    GAGCCCAATG TTACTTGGAA GAAGGATGGG ATTCCAATAA ACCATAATAA TAGTCGATAC
    ACGATTTCTG CAGGCAAACT CTTTATTGGC AAAGCACTAA AGACGGATTC AGGACTATAT
    TCCTGCTTAG CATCAAATCA AGTGGGAGAA AGAGAGAGTA GAGCTGCTAA AATCGTTGTA
    CTGGAGAAGC CCATGTTTAC ATATAAACCC AGTGATGTGA CGGCAAAGTT TGGAAGCACC
    GTGCAGTTTG GTTGTGGAGC ACAAGGAGAC CCAAAGCCCA CCTTTCACTG GAGCAAAGAA
    AATGGAGACC TTCCACAAGG GAGGTATGAA ATTACCAATG AAAATACATT AAGAATCCGA
    TCTGTAACCT TGTATGACTC TGGAAAATAT ATCTGCACTG CAGGAAATCA GCTAGAAACA
    GTATCTGCCA AGGCATTGCT AACTGTTCAA GATCCTTTGG ATACTGGTCA GATAGAAAAG
    GAGAGACAAA TCTATAGAGA ATTAATGGGT GTTCGTGTTC ATTTAGACAA CATCACAGCA
    CTTCCAAGCG CATCAGTCTA TGTGCACTGG CAGGTCCTAA CGGAATCCCC CTTTACTGAA
    GGATATAGTG TATTTTACCG CACACTTTCT TCTACTGAAT CTGAGTGGAC AGAGTGGACA
    AAGTCTAGGA TCAATGAGTA CAGCACTGTT ATTCCATCTC TATGGAGGGG ACAGAAATAT
    GAGTTTAAGA TCCGACCTTT TGCTGGGAAA AATTATGGAC CTGACAGCAA TATTAAACAT
    CTCAGAATAC CTGAGGAAGT TCCAAATATT GCTCCACAGA ACATCAATGT GACTGCTGTG
    GAAGGAGGAA ACGGTACAAT AGTTGTGAGC TGGGAACCCC CACCAAAAAA CATTCACAGC
    AGTAACATAA AAGGTTATAA GGTCTGGTGT TTTGAGAATG AAACACATCC TAAAGCAGAC
    TGGATAGTGG ATGAAGAAAC CAAGAGTCTA GAAATTCCCA TGTTGGCCTC CGGCATTAAA
    TATCAAGTCC AAGTAGCGGC TATAAATGAT GCAGGAATTG GAATGCCAAG CATACCTAAA
    TACATAATGA TAGACTCACC AGAAGTGAAG GAGGTAAAAG ATGAAAATAT ATATTTTGAG
    CTAATCCTGC AAGTTGTGCG TCACCCAGCA TTCATTGGAA CTGTAGGAAC CGTGGCCTGG
    ATATTATTAA TGGTTGCAGC CGTGTATGTG TGTCAGCGTC ATTCTAAGCG CTATAGCACA
    AAACAACACA GTATATTAGG AAACAGTCTT TACCGGTTTG CGAGTGAAGA TACCATAATA
    AAGCACAGGA TGGACATTAG TGATTCCCCA TGGCTTTCAC ACACATGGAA ATCCGCCTCC
    TGCTCGCGAA ACTACAGCAG CACAATCAGT ATGAACAGCC AATTACTGTG GTCTGAAACT
    AAAGACACGG CAGATTTCCA TAAATCAACA ATGTCGTTTG AGCGCAAAAG TGAGGGTAGT
    CGAAGCCAGA TTATTCCGCT GGTGCCAGAC AGCAACAGCT TGTATGGAAC AATGTATGTC
    GACCTTCCAG GAAAAGATAT GACCACATTT CAGCGTCTGC CTTCTGACAA ATCTCAAGGC
    ATGGGGAGAC ATTTTAAAAC TCCTGACCCT CTAGGATTGC TTCATCACAG GCCATTGCCA
    TCATATTTGA ATGATTTCAG TGGCAGCAAT GATATGAATG GAGGAACTAG AAGCAAGTTG
    CCATGGCAAC CAAATATTAA CAATAATCCT GATATCTCAT TTAAGGACTC ATGGAGCAAA
    AACTGCAAGA AAGAGCTTCA ACATGCAAAT AGTGCTCCTT TGTCCCCTTA TTGCCAAGCA
    CCCAAACAGG GTGACAGTGT ACCAAGCATT CAGCATTTAA ACATCAAGAA AGTAGGAAAA
    GGGGACTATG CTAAAGTAAT GAAGACTTTC AGTTCCCCTA AAATCCTTCA CTACACCACC
    TCTCTCCAAG TGATGGATCT TCTACCCTAT ACACCTTCTC TCCCACCACC TCCAGTTCCT
    CCTCCAGATG AAAATCCAAT TCACAATGAC AAGGATAAAA TTGGTTGCAG TGTCTCATCA
    TATTCCGTGC GCAATCAAGA AAAGCAAGGC CATAAAATCA ACAGCAAGAA AAAGAGTCCC
    CCTGATACAC TGAACATTAA ACCAGCTTCA GAATGCTTCC CAATAAATGA TGATGGAGAT
    AATGTGTTAA CTCCAGAAGA TGTGGCACAA TATCTTGAAC TCAGTGATCA AGGGCAAAAT
    ACAAGGCATA AATCAGAATA TGATGCTTCC CTTCCAAGGC CTTTCTCTCC TTCTCAAACA
    TATGGCTACA TTTGCAGCCC GATACCTTCA GAAGCTGATG TTGTTGATGA AGATGATGAC
    CTTGATTTAG AGGACATCGG TTCATTAAAG TCTTATCGCA AGTACTGTGA AACTCCAACC
    TCCAGTATCA GTGAATATGA GAGCTCTATG GCCGGATCTC TTGTTAATGG CTGGGGTTCT
    GTTTCAGAAG ATAATTATAC CAGTGCTAGA TGCAGCATGG TCAGCTCCTC TGATGGGTCC
    TTTTTAATGG ATGCAAACTT CGCCAAGGCT CTTGCAGTTG CAGTTGACAG TTTTTGCTTT
    GGTGGATCTC AGACTGAGAC TGCTGGAAGA GATACGTTTT ATGCAGATCT TTCTCCGTCA
    GTGTCTCCTT TGGATGGAAT ATTAAACCAA CAAAGTGGGG AAAATACAGA TGTTACCAAA
    AAGAAATCCA GAGTTAATCA TTTGCCTGTT TTAGACTGGA ACATTGACTG GATGGATGAA
    ATGGAAGCCA GGTACAGCAG GAAAAATGAC ATGAAACATC ATTTTCCTTT TGATAAAAAG
    ATGGATCCTT TCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822438.1
    CDS125..3622
    Translation

    Target ORF information:

    RefSeq Version XM_012966984.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis roundabout guidance receptor 4 (robo4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966984.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAATTTTG CCTACTTTCT TCCAATTTTT GTACTTTTTC CCTTGGGATG GTGTTCACAG 
    GTGAGCTCCT GCCAATGTCA TCAGCCAGTC ATTAATAAAG AACATGGCCT GGAAGCTACA
    CGTAGACAGA CCCGGCATGT TCAGCACCAC CGGTCAACAA GAAGGCACCG TATTAGAGGC
    TCAAAAACTC GATCAGAGGA TTTTCCACCT CGTATTATCG AACACCCTTC AGATTTGTTT
    GTGAAGAAGG ACAAGCCAGC CACATTGTAT TGTCGAGCAG AGGGCAATCC ACAGCCCACA
    ATAGAATGGT ACCGTAATGG AGAGCATGTG GAGACCAGCA CAGATGATAA GACCCAGCGT
    TCCTATGTTC AGTTGCCGGA GGGACCACTC TTCTTCTTCA GCCTTAATCA ACGAAAAGGC
    AAATCTGATG AAGGGATTTA TACTTGTGTG GCTAGAAACC ATCTAGGAAC GGCATTTAGT
    AGAAATGCTT CTCTTTATGT TGCTGCTCTA AGGGATGATT TTCGTCTAAA TCCATCTGAT
    GCTGTTGTGG CCGTGGGGAA GCAACTTGAG ATAGAATGTT TGCCTCCAAA AGGTCATCCT
    GAGCCCAATG TTACTTGGAA GAAGGATGGG ATTCCAATAA ACCATAATAA TAGTCGATAC
    ACGATTTCTG CAGGCAAACT CTTTATTGGC AAAGCACTAA AGACGGATTC AGGACTATAT
    TCCTGCTTAG CATCAAATCA AGTGGGAGAA AGAGAGAGTA GAGCTGCTAA AATCGTTGTA
    CTGGAGAAGC CCATGTTTAC ATATAAACCC AGTGATGTGA CGGCAAAGTT TGGAAGCACC
    GTGCAGTTTG GTTGTGGAGC ACAAGGAGAC CCAAAGCCCA CCTTTCACTG GAGCAAAGAA
    AATGGAGACC TTCCACAAGG GAGGTATGAA ATTACCAATG AAAATACATT AAGAATCCGA
    TCTGTAACCT TGTATGACTC TGGAAAATAT ATCTGCACTG CAGGAAATCA GCTAGAAACA
    GTATCTGCCA AGGCATTGCT AACTGTTCAA GATCCTTTGG ATACTGGTCA GATAGAAAAG
    GAGAGACAAA TCTATAGAGA ATTAATGGGT GTTCGTGTTC ATTTAGACAA CATCACAGCA
    CTTCCAAGCG CATCAGTCTA TGTGCACTGG CAGGTCCTAA CGGAATCCCC CTTTACTGAA
    GGATATAGTG TATTTTACCG CACACTTTCT TCTACTGAAT CTGAGTGGAC AGAGTGGACA
    AAGTCTAGGA TCAATGAGTA CAGCACTGTT ATTCCATCTC TATGGAGGGG ACAGAAATAT
    GAGTTTAAGA TCCGACCTTT TGCTGGGAAA AATTATGGAC CTGACAGCAA TATTAAACAT
    CTCAGAATAC CTGAGGAAGT TCCAAATATT GCTCCACAGA ACATCAATGT GACTGCTGTG
    GAAGGAGGAA ACGGTACAAT AGTTGTGAGC TGGGAACCCC CACCAAAAAA CATTCACAGC
    AGTAACATAA AAGGTTATAA GGTCTGGTGT TTTGAGAATG AAACACATCC TAAAGCAGAC
    TGGATAGTGG ATGAAGAAAC CAAGAGTCTA GAAATTCCCA TGTTGGCCTC CGGCATTAAA
    TATCAAGTCC AAGTAGCGGC TATAAATGAT GCAGGAATTG GAATGCCAAG CATACCTAAA
    TACATAATGA TAGACTCACC AGAAGTGAAG GAGGTAAAAG ATGAAAATAT ATATTTTGAG
    CTAATCCTGC AAGTTGTGCG TCACCCAGCA TTCATTGGAA CTGTAGGAAC CGTGGCCTGG
    ATATTATTAA TGGTTGCAGC CGTGTATGTG TGTCAGCGTC ATTCTAAGCG CTATAGCACA
    AAACAACACA GTATATTAGG AAACAGTCTT TACCGGTTTG CGAGTGAAGA TACCATAATA
    AAGCACAGGA TGGACATTAG TGATTCCCCA TGGCTTTCAC ACACATGGAA ATCCGCCTCC
    TGCTCGCGAA ACTACAGCAG CACAATCAGT ATGAACAGCC AATTACTGTG GTCTGAAACT
    AAAGACACGG CAGATTTCCA TAAATCAACA ATGTCGTTTG AGCGCAAAAG TGAGGGTAGT
    CGAAGCCAGA TTATTCCGCT GGTGCCAGAC AGCAACAGCT TGTATGGAAC AATGTATGTC
    GACCTTCCAG GAAAAGATAT GACCACATTT CAGCGTCTGC CTTCTGACAA ATCTCAAGGC
    ATGGGGAGAC ATTTTAAAAC TCCTGACCCT CTAGGATTGC TTCATCACAG GCCATTGCCA
    TCATATTTGA ATGATTTCAG TGGCAGCAAT GATATGAATG GAGGAACTAG AAGCAAGTTG
    CCATGGCAAC CAAATATTAA CAATAATCCT GATATCTCAT TTAAGGACTC ATGGAGCAAA
    AACTGCAAGA AAGAGCTTCA ACATGCAAAT AGTGCTCCTT TGTCCCCTTA TTGCCAAGCA
    CCCAAACAGG GTGACAGTGT ACCAAGCATT CAGCATTTAA ACATCAAGAA AGTAGGAAAA
    GGGGACTATG CTAAAGTAAT GAAGACTTTC AGTTCCCCTA AAATCCTTCA CTACACCACC
    TCTCTCCAAG TGATGGATCT TCTACCCTAT ACACCTTCTC TCCCACCACC TCCAGTTCCT
    CCTCCAGATG AAAATCCAAT TCACAATGAC AAGGATAAAA TTGGTTGCAG TGTCTCATCA
    TATTCCGTGC GCAATCAAGA AAAGCAAGGC CATAAAATCA ACAGCAAGAA AAAGAGTCCC
    CCTGATACAC TGAACATTAA ACCAGCTTCA GAATGCTTCC CAATAAATGA TGATGGAGAT
    AATGTGTTAA CTCCAGAAGA TGTGGCACAA TATCTTGAAC TCAGTGATCA AGGGCAAAAT
    ACAAGGCATA AATCAGAATA TGATGCTTCC CTTCCAAGGC CTTTCTCTCC TTCTCAAACA
    TATGGCTACA TTTGCAGCCC GATACCTTCA GAAGCTGATG TTGTTGATGA AGATGATGAC
    CTTGATTTAG AGGACATCGG TTCATTAAAG TCTTATCGCA AGTACTGTGA AACTCCAACC
    TCCAGTATCA GTGAATATGA GAGCTCTATG GCCGGATCTC TTGTTAATGG CTGGGGTTCT
    GTTTCAGAAG ATAATTATAC CAGTGCTAGA TGCAGCATGG TCAGCTCCTC TGATGGGTCC
    TTTTTAATGG ATGCAAACTT CGCCAAGGCT CTTGCAGTTG CAGTTGACAG TTTTTGCTTT
    GGTGGATCTC AGACTGAGAC TGCTGGAAGA GATACGTTTT ATGCAGATCT TTCTCCGTCA
    GTGTCTCCTT TGGATGGAAT ATTAAACCAA CAAAGTGGGG AAAATACAGA TGTTACCAAA
    AAGAAATCCA GAGTTAATCA TTTGCCTGTT TTAGACTGGA ACATTGACTG GATGGATGAA
    ATGGAAGCCA GGTACAGCAG GAAAAATGAC ATGAAACATC ATTTTCCTTT TGATAAAAAG
    ATGGATCCTT TCAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.