ACO1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACO1
Entrez Gene ID	48
Full Name	aconitase 1
Synonyms	ACONS,HEL60,IREB1,IREBP,IREBP1,IRP1
General protein information	Preferred Names aconitase 1 Names cytoplasmic aconitate hydratase IRE-BP 1 aconitase 1, soluble aconitate hydratase, cytoplasmic citrate hydro-lyase cytosplasmic aconitase epididymis luminal protein 60 ferritin repressor protein iron regulatory protein 1 iron-responsive element-binding protein 1 soluble aconitase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9p21.1
Summary	The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014].
Disorder MIM:	100880

mRNA and Protein(s)

mRNA	Protein	Name
NM_001362840.2	NP_001349769.1	cytoplasmic aconitate hydratase
XM_011517888.1	XP_011516190.1	cytoplasmic aconitate hydratase isoform X1
NM_001278352.1	NP_001265281.1	cytoplasmic aconitate hydratase
NM_002197.2	NP_002188.1	cytoplasmic aconitate hydratase
NM_001362840.1	NP_001349769.1	cytoplasmic aconitate hydratase
NM_002197.3	NP_002188.1	cytoplasmic aconitate hydratase
XM_005251476.1	XP_005251533.1	cytoplasmic aconitate hydratase isoform X1
XM_011517888.2	XP_011516190.1	cytoplasmic aconitate hydratase isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ACO1	NP_002188.1
Pan troglodytes (chimpanzee)	ACO1	XP_001156102.1
Drosophila melanogaster (fruit fly)	Irp-1A	NP_477371.1
Arabidopsis thaliana (thale cress)	ACO1	NP_195308.1
	ACO1	XP_001103761.1
Bos taurus (cattle)	ACO1	NP_001069059.1
Arabidopsis thaliana (thale cress)	ACO3	NP_178634.2
Caenorhabditis elegans (roundworm)	aco-1	NP_509898.1
Oryza sativa (rice)	Os03g0136900	NP_001048898.1
Canis lupus familiaris (dog)	ACO1	XP_538698.2
Rattus norvegicus (Norway rat)	Aco1	NP_059017.1
Danio rerio (zebrafish)	aco1	NP_001030155.1
Drosophila melanogaster (fruit fly)	Irp-1B	NP_524303.2
Xenopus tropicalis (tropical clawed frog)	aco1	NP_001135643.1
Mus musculus (house mouse)	Aco1	NP_031412.2
Gallus gallus (chicken)	ACO1	NP_001025707.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007258	XP_308544.4

Gene Ontology
Bibliography

Related articles in PubMed

Evaluation of the iron regulatory protein-1 interactome.
Connell GJ, Danial JS, Haastruthers CX
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine31(1)139-146(2018 Feb)

A synergistic role of IRP1 and FBXL5 proteins in coordinating iron metabolism during cell proliferation.
Johnson NB, Deck KM, Nizzi CP, Eisenstein RS
The Journal of biological chemistry292(38)15976-15989(2017 09)

Pathological changes in Sertoli cells and dysregulation of divalent metal transporter 1 with iron responsive element in?the testes of idiopathic azoospermia patients.
Jing T, Wang P, Liu Y, Zhao J, Niu X, Wang X
Andrologia(2017 Aug)

Biogenesis and functions of mammalian iron-sulfur proteins in the regulation of iron homeostasis and pivotal metabolic pathways.
Rouault TA, Maio N
The Journal of biological chemistry292(31)12744-12753(2017 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

regulatory circuit involving FBXL5 and CIA acts through both IRPs to control iron metabolism and promote optimal cell growth
Title: A synergistic role of IRP1 and FBXL5 proteins in coordinating iron metabolism during cell proliferation.

Data suggest that Fe-S cluster of IRP1 plays role in sensing/regulating cellular iron homeostasis; IRP1 alternates between function as cytosolic aconitase [which contains Fe(4)-S(4) cluster in active-site cleft] and function as apoenzyme [which lacks Fe-S cluster] that binds to iron response element stem-loop structures present in several iron regulatory protein transcripts. [REVIEW]
Title: Biogenesis and functions of mammalian iron-sulfur proteins in the regulation of iron homeostasis and pivotal metabolic pathways.

The SNP rs7874815 in the ACO1 gene was strongly associated with survival in pancreatic cancer.
Title: Circulating Metabolites and Survival Among Patients With Pancreatic Cancer.

Our results confirm the observation that mitoNEET is important in transferring the iron sulfur clusters to the cytosolic aconitase in living cells and the His-87 ligand in mitoNEET plays important role in this process.
Title: His-87 ligand in mitoNEET is crucial for the transfer of iron sulfur clusters from mitochondria to cytosolic aconitase.

These in vitro studies suggest that human GLRX3 is important for cytosolic Fe-S protein maturation.
Title: Human glutaredoxin 3 can bind and effectively transfer [4Fe-4S] cluster to apo-iron regulatory protein 1.

The following ACO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17506	NM_002197.3 Latest version!	Homo sapiens aconitase 1 (ACO1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu17506	NM_001278352.1 Latest version!	Homo sapiens aconitase 1 (ACO1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu17506	NM_001362840.2 Latest version!	Homo sapiens aconitase 1 (ACO1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu17506	XM_005251476.1 Latest version!	Homo sapiens aconitase 1 (ACO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu17506	XM_011517888.2 Latest version!	Homo sapiens aconitase 1 (ACO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
View Old Accession Versions >>
OHu17506	NM_001362840.1	Homo sapiens aconitase 1 (ACO1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu17506	NM_002197.2	Homo sapiens aconitase 1 (ACO1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu17506	XM_011517888.1	Homo sapiens aconitase 1 (ACO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACO1 ( NM_001362840.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001349769.1 Homo sapiens aconitase 1 (ACO1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu17506
Clone ID Related Accession (Same CDS sequence)	NM_001362840.2 , XM_011517888.1 , NM_001278352.1 , NM_002197.2 , NM_001362840.1 , NM_002197.3 , XM_005251476.1 , XM_011517888.2
Accession Version	NM_001362840.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	cytoplasmic aconitate hydratase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA780353.1, BP287929.1, AF261088.1, BC018103.1 and AL161783.20. On May 31, 2019 this sequence version replaced NM_001362840.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.139330.1, SRR1803615.264975.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 8041788 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCAACC CATTCGCACA CCTTGCTGAG CCATTGGATC CTGTACAACC AGGAAAGAAA 
TTCTTCAATT TGAATAAATT GGAGGATTCA AGATATGGGC GCTTACCATT TTCGATCAGA 
GTTCTTCTGG AAGCAGCCAT TCGGAATTGT GATGAGTTTT TGGTGAAGAA ACAGGATATT 
GAAAATATTC TACATTGGAA TGTCACGCAG CACAAGAACA TAGAAGTGCC ATTTAAGCCT 
GCTCGTGTCA TCCTGCAGGA CTTTACGGGT GTGCCCGCTG TGGTTGACTT TGCTGCAATG 
CGTGATGCTG TGAAAAAGTT AGGAGGAGAT CCAGAGAAAA TAAACCCTGT CTGCCCTGCT 
GATCTTGTAA TAGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAATT TGAAAGAAAT AGAGAGCGAT TTGAATTTTT AAAGTGGGGT 
TCCCAGGCTT TTCACAACAT GCGGATTATT CCCCCTGGCT CAGGAATCAT CCACCAGGTG 
AATTTGGAAT ATTTGGCAAG AGTGGTATTT GATCAGGATG GATATTATTA CCCAGACAGC 
CTCGTGGGCA CAGACTCGCA CACTACCATG ATTGATGGCT TGGGCATTCT TGGTTGGGGT 
GTCGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CAATCAGTAT GGTGCTTCCT 
CAGGTGATTG GCTACAGGCT GATGGGGAAG CCCCACCCTC TGGTAACATC CACTGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGCCAG GTTGGGGTAG TGGGCAAATT TGTCGAGTTC 
TTCGGGCCTG GAGTAGCCCA GTTGTCCATT GCTGACCGAG CTACGATTGC TAACATGTGT 
CCAGAGTACG GAGCAACTGC TGCCTTTTTC CCAGTTGATG AAGTTAGTAT CACGTACCTG 
GTGCAAACAG GTCGTGATGA AGAAAAATTA AAGTATATTA AAAAATATCT TCAGGCTGTA 
GGAATGTTTC GAGATTTCAA TGACCCTTCT CAAGACCCAG ACTTCACCCA GGTTGTGGAA 
TTAGATTTGA AAACAGTAGT GCCTTGCTGT AGTGGACCCA AAAGGCCTCA GGACAAAGTT 
GCTGTGTCCG ACATGAAAAA GGACTTTGAG AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGATTCCAAG TTGCTCCTGA ACATCATAAT GACCATAAGA CCTTTATCTA TGATAACACT 
GAATTCACCC TTGCTCATGG TTCTGTGGTC ATTGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCGT CTGTGATGTT AGGGGCAGGA TTGTTAGCAA AGAAAGCTGT GGATGCTGGC 
CTGAACGTGA TGCCTTACAT CAAAACTAGC CTGTCTCCTG GGAGTGGCGT GGTCACCTAC 
TACCTACAAG AAAGCGGAGT CATGCCTTAT CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT TGGCAACAGT GGGCCTTTAC CTGAACCTGT GGTAGAAGCC 
ATCACACAGG GAGACCTTGT AGCTGTTGGA GTACTATCTG GAAACAGGAA TTTTGAAGGT 
CGAGTTCACC CCAACACCCG GGCCAACTAT TTAGCCTCTC CCCCCTTAGT AATAGCATAT 
GCAATTGCTG GAACCATCAG AATCGACTTT GAGAAAGAGC CATTGGGAGT AAATGCAAAG 
GGACAGCAGG TATTTCTGAA AGATATCTGG CCGACTAGAG ACGAGATCCA GGCAGTGGAG 
CGTCAGTATG TCATCCCGGG GATGTTTAAG GAAGTCTATC AGAAAATAGA GACTGTGAAT 
GAAAGCTGGA ATGCCTTAGC AACCCCATCA GATAAGCTGT TTTTCTGGAA TTCCAAATCT 
ACGTATATCA AATCACCACC ATTCTTTGAA AACCTGACTT TGGATCTTCA GCCCCCTAAA 
TCTATAGTGG ATGCCTATGT GCTGCTAAAT TTGGGAGATT CGGTAACAAC TGACCACATC 
TCCCCAGCTG GAAATATTGC AAGAAACAGT CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTAACTCCAC GAGAATTCAA CTCCTATGGC TCCCGCCGAG GTAATGACGC CGTCATGGCA 
CGGGGAACAT TTGCCAACAT TCGCTTGTTA AACAGATTTT TGAACAAGCA GGCACCACAG 
ACTATCCATC TGCCTTCTGG GGAAATCCTT GATGTGTTTG ATGCTGCTGA GCGGTACCAG 
CAGGCAGGCC TTCCCCTGAT CGTTCTGGCT GGCAAAGAGT ACGGTGCAGG CAGCTCCCGA 
GACTGGGCAG CTAAGGGCCC TTTCCTGCTG GGAATCAAAG CCGTCCTGGC CGAGAGCTAC 
GAGCGCATTC ACCGCAGTAA CCTGGTTGGG ATGGGTGTGA TCCCACTTGA ATATCTCCCT 
GGTGAGAATG CAGATGCCCT GGGGCTCACA GGGCAAGAAC GATACACTAT CATTATTCCA 
GAAAACCTCA AACCACAAAT GAAAGTCCAG GTCAAGCTGG ATACTGGCAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC TGATGTGGAG CTCACTTATT TCCTCAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349769.1
CDS	184..2853
Translation	MSNPFAHLAEPLDPVQPGKKFFNLNKLEDSRYGRLPFSIRVLLEAAIRNCDEFLVKKQDIENILHWNVTQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNRERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGILGWGVGGIEAEAVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSITYLVQTGRDEEKLKYIKKYLQAVGMFRDFNDPSQDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPEHHNDHKTFIYDNTEFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLNVMPYIKTSLSPGSGVVTYYLQESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMIRKMAK

Target ORF information:

RefSeq Version	NM_001362840.2
Organism	Homo sapiens(human)
Definition	Homo sapiens aconitase 1 (ACO1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362840.2

ORF Insert Sequence:

ATGAGCAACC CATTCGCACA CCTTGCTGAG CCATTGGATC CTGTACAACC AGGAAAGAAA 
TTCTTCAATT TGAATAAATT GGAGGATTCA AGATATGGGC GCTTACCATT TTCGATCAGA 
GTTCTTCTGG AAGCAGCCAT TCGGAATTGT GATGAGTTTT TGGTGAAGAA ACAGGATATT 
GAAAATATTC TACATTGGAA TGTCACGCAG CACAAGAACA TAGAAGTGCC ATTTAAGCCT 
GCTCGTGTCA TCCTGCAGGA CTTTACGGGT GTGCCCGCTG TGGTTGACTT TGCTGCAATG 
CGTGATGCTG TGAAAAAGTT AGGAGGAGAT CCAGAGAAAA TAAACCCTGT CTGCCCTGCT 
GATCTTGTAA TAGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAATT TGAAAGAAAT AGAGAGCGAT TTGAATTTTT AAAGTGGGGT 
TCCCAGGCTT TTCACAACAT GCGGATTATT CCCCCTGGCT CAGGAATCAT CCACCAGGTG 
AATTTGGAAT ATTTGGCAAG AGTGGTATTT GATCAGGATG GATATTATTA CCCAGACAGC 
CTCGTGGGCA CAGACTCGCA CACTACCATG ATTGATGGCT TGGGCATTCT TGGTTGGGGT 
GTCGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CAATCAGTAT GGTGCTTCCT 
CAGGTGATTG GCTACAGGCT GATGGGGAAG CCCCACCCTC TGGTAACATC CACTGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGCCAG GTTGGGGTAG TGGGCAAATT TGTCGAGTTC 
TTCGGGCCTG GAGTAGCCCA GTTGTCCATT GCTGACCGAG CTACGATTGC TAACATGTGT 
CCAGAGTACG GAGCAACTGC TGCCTTTTTC CCAGTTGATG AAGTTAGTAT CACGTACCTG 
GTGCAAACAG GTCGTGATGA AGAAAAATTA AAGTATATTA AAAAATATCT TCAGGCTGTA 
GGAATGTTTC GAGATTTCAA TGACCCTTCT CAAGACCCAG ACTTCACCCA GGTTGTGGAA 
TTAGATTTGA AAACAGTAGT GCCTTGCTGT AGTGGACCCA AAAGGCCTCA GGACAAAGTT 
GCTGTGTCCG ACATGAAAAA GGACTTTGAG AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGATTCCAAG TTGCTCCTGA ACATCATAAT GACCATAAGA CCTTTATCTA TGATAACACT 
GAATTCACCC TTGCTCATGG TTCTGTGGTC ATTGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCGT CTGTGATGTT AGGGGCAGGA TTGTTAGCAA AGAAAGCTGT GGATGCTGGC 
CTGAACGTGA TGCCTTACAT CAAAACTAGC CTGTCTCCTG GGAGTGGCGT GGTCACCTAC 
TACCTACAAG AAAGCGGAGT CATGCCTTAT CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT TGGCAACAGT GGGCCTTTAC CTGAACCTGT GGTAGAAGCC 
ATCACACAGG GAGACCTTGT AGCTGTTGGA GTACTATCTG GAAACAGGAA TTTTGAAGGT 
CGAGTTCACC CCAACACCCG GGCCAACTAT TTAGCCTCTC CCCCCTTAGT AATAGCATAT 
GCAATTGCTG GAACCATCAG AATCGACTTT GAGAAAGAGC CATTGGGAGT AAATGCAAAG 
GGACAGCAGG TATTTCTGAA AGATATCTGG CCGACTAGAG ACGAGATCCA GGCAGTGGAG 
CGTCAGTATG TCATCCCGGG GATGTTTAAG GAAGTCTATC AGAAAATAGA GACTGTGAAT 
GAAAGCTGGA ATGCCTTAGC AACCCCATCA GATAAGCTGT TTTTCTGGAA TTCCAAATCT 
ACGTATATCA AATCACCACC ATTCTTTGAA AACCTGACTT TGGATCTTCA GCCCCCTAAA 
TCTATAGTGG ATGCCTATGT GCTGCTAAAT TTGGGAGATT CGGTAACAAC TGACCACATC 
TCCCCAGCTG GAAATATTGC AAGAAACAGT CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTAACTCCAC GAGAATTCAA CTCCTATGGC TCCCGCCGAG GTAATGACGC CGTCATGGCA 
CGGGGAACAT TTGCCAACAT TCGCTTGTTA AACAGATTTT TGAACAAGCA GGCACCACAG 
ACTATCCATC TGCCTTCTGG GGAAATCCTT GATGTGTTTG ATGCTGCTGA GCGGTACCAG 
CAGGCAGGCC TTCCCCTGAT CGTTCTGGCT GGCAAAGAGT ACGGTGCAGG CAGCTCCCGA 
GACTGGGCAG CTAAGGGCCC TTTCCTGCTG GGAATCAAAG CCGTCCTGGC CGAGAGCTAC 
GAGCGCATTC ACCGCAGTAA CCTGGTTGGG ATGGGTGTGA TCCCACTTGA ATATCTCCCT 
GGTGAGAATG CAGATGCCCT GGGGCTCACA GGGCAAGAAC GATACACTAT CATTATTCCA 
GAAAACCTCA AACCACAAAT GAAAGTCCAG GTCAAGCTGG ATACTGGCAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC TGATGTGGAG CTCACTTATT TCCTCAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACO1 ( NM_001278352.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265281.1 Homo sapiens aconitase 1 (ACO1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17506
Clone ID Related Accession (Same CDS sequence)	NM_001362840.2 , XM_011517888.1 , NM_001278352.1 , NM_002197.2 , NM_001362840.1 , NM_002197.3 , XM_005251476.1 , XM_011517888.2
Accession Version	NM_001278352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-20
Organism	Homo sapiens(human)
Product	cytoplasmic aconitate hydratase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA780353.1, DA103947.1, BP287929.1, AF261088.1 and BC018103.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.164875.1, SRR1660803.156520.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 8041788 ##RefSeq-Attributes-END##

ATGAGCAACC CATTCGCACA CCTTGCTGAG CCATTGGATC CTGTACAACC AGGAAAGAAA 
TTCTTCAATT TGAATAAATT GGAGGATTCA AGATATGGGC GCTTACCATT TTCGATCAGA 
GTTCTTCTGG AAGCAGCCAT TCGGAATTGT GATGAGTTTT TGGTGAAGAA ACAGGATATT 
GAAAATATTC TACATTGGAA TGTCACGCAG CACAAGAACA TAGAAGTGCC ATTTAAGCCT 
GCTCGTGTCA TCCTGCAGGA CTTTACGGGT GTGCCCGCTG TGGTTGACTT TGCTGCAATG 
CGTGATGCTG TGAAAAAGTT AGGAGGAGAT CCAGAGAAAA TAAACCCTGT CTGCCCTGCT 
GATCTTGTAA TAGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAATT TGAAAGAAAT AGAGAGCGAT TTGAATTTTT AAAGTGGGGT 
TCCCAGGCTT TTCACAACAT GCGGATTATT CCCCCTGGCT CAGGAATCAT CCACCAGGTG 
AATTTGGAAT ATTTGGCAAG AGTGGTATTT GATCAGGATG GATATTATTA CCCAGACAGC 
CTCGTGGGCA CAGACTCGCA CACTACCATG ATTGATGGCT TGGGCATTCT TGGTTGGGGT 
GTCGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CAATCAGTAT GGTGCTTCCT 
CAGGTGATTG GCTACAGGCT GATGGGGAAG CCCCACCCTC TGGTAACATC CACTGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGCCAG GTTGGGGTAG TGGGCAAATT TGTCGAGTTC 
TTCGGGCCTG GAGTAGCCCA GTTGTCCATT GCTGACCGAG CTACGATTGC TAACATGTGT 
CCAGAGTACG GAGCAACTGC TGCCTTTTTC CCAGTTGATG AAGTTAGTAT CACGTACCTG 
GTGCAAACAG GTCGTGATGA AGAAAAATTA AAGTATATTA AAAAATATCT TCAGGCTGTA 
GGAATGTTTC GAGATTTCAA TGACCCTTCT CAAGACCCAG ACTTCACCCA GGTTGTGGAA 
TTAGATTTGA AAACAGTAGT GCCTTGCTGT AGTGGACCCA AAAGGCCTCA GGACAAAGTT 
GCTGTGTCCG ACATGAAAAA GGACTTTGAG AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGATTCCAAG TTGCTCCTGA ACATCATAAT GACCATAAGA CCTTTATCTA TGATAACACT 
GAATTCACCC TTGCTCATGG TTCTGTGGTC ATTGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCGT CTGTGATGTT AGGGGCAGGA TTGTTAGCAA AGAAAGCTGT GGATGCTGGC 
CTGAACGTGA TGCCTTACAT CAAAACTAGC CTGTCTCCTG GGAGTGGCGT GGTCACCTAC 
TACCTACAAG AAAGCGGAGT CATGCCTTAT CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT TGGCAACAGT GGGCCTTTAC CTGAACCTGT GGTAGAAGCC 
ATCACACAGG GAGACCTTGT AGCTGTTGGA GTACTATCTG GAAACAGGAA TTTTGAAGGT 
CGAGTTCACC CCAACACCCG GGCCAACTAT TTAGCCTCTC CCCCCTTAGT AATAGCATAT 
GCAATTGCTG GAACCATCAG AATCGACTTT GAGAAAGAGC CATTGGGAGT AAATGCAAAG 
GGACAGCAGG TATTTCTGAA AGATATCTGG CCGACTAGAG ACGAGATCCA GGCAGTGGAG 
CGTCAGTATG TCATCCCGGG GATGTTTAAG GAAGTCTATC AGAAAATAGA GACTGTGAAT 
GAAAGCTGGA ATGCCTTAGC AACCCCATCA GATAAGCTGT TTTTCTGGAA TTCCAAATCT 
ACGTATATCA AATCACCACC ATTCTTTGAA AACCTGACTT TGGATCTTCA GCCCCCTAAA 
TCTATAGTGG ATGCCTATGT GCTGCTAAAT TTGGGAGATT CGGTAACAAC TGACCACATC 
TCCCCAGCTG GAAATATTGC AAGAAACAGT CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTAACTCCAC GAGAATTCAA CTCCTATGGC TCCCGCCGAG GTAATGACGC CGTCATGGCA 
CGGGGAACAT TTGCCAACAT TCGCTTGTTA AACAGATTTT TGAACAAGCA GGCACCACAG 
ACTATCCATC TGCCTTCTGG GGAAATCCTT GATGTGTTTG ATGCTGCTGA GCGGTACCAG 
CAGGCAGGCC TTCCCCTGAT CGTTCTGGCT GGCAAAGAGT ACGGTGCAGG CAGCTCCCGA 
GACTGGGCAG CTAAGGGCCC TTTCCTGCTG GGAATCAAAG CCGTCCTGGC CGAGAGCTAC 
GAGCGCATTC ACCGCAGTAA CCTGGTTGGG ATGGGTGTGA TCCCACTTGA ATATCTCCCT 
GGTGAGAATG CAGATGCCCT GGGGCTCACA GGGCAAGAAC GATACACTAT CATTATTCCA 
GAAAACCTCA AACCACAAAT GAAAGTCCAG GTCAAGCTGG ATACTGGCAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC TGATGTGGAG CTCACTTATT TCCTCAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265281.1
CDS	228..2897
Translation	MSNPFAHLAEPLDPVQPGKKFFNLNKLEDSRYGRLPFSIRVLLEAAIRNCDEFLVKKQDIENILHWNVTQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNRERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGILGWGVGGIEAEAVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSITYLVQTGRDEEKLKYIKKYLQAVGMFRDFNDPSQDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPEHHNDHKTFIYDNTEFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLNVMPYIKTSLSPGSGVVTYYLQESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMIRKMAK

Target ORF information:

RefSeq Version	NM_001278352.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aconitase 1 (ACO1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278352.1

ORF Insert Sequence:

ATGAGCAACC CATTCGCACA CCTTGCTGAG CCATTGGATC CTGTACAACC AGGAAAGAAA 
TTCTTCAATT TGAATAAATT GGAGGATTCA AGATATGGGC GCTTACCATT TTCGATCAGA 
GTTCTTCTGG AAGCAGCCAT TCGGAATTGT GATGAGTTTT TGGTGAAGAA ACAGGATATT 
GAAAATATTC TACATTGGAA TGTCACGCAG CACAAGAACA TAGAAGTGCC ATTTAAGCCT 
GCTCGTGTCA TCCTGCAGGA CTTTACGGGT GTGCCCGCTG TGGTTGACTT TGCTGCAATG 
CGTGATGCTG TGAAAAAGTT AGGAGGAGAT CCAGAGAAAA TAAACCCTGT CTGCCCTGCT 
GATCTTGTAA TAGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAATT TGAAAGAAAT AGAGAGCGAT TTGAATTTTT AAAGTGGGGT 
TCCCAGGCTT TTCACAACAT GCGGATTATT CCCCCTGGCT CAGGAATCAT CCACCAGGTG 
AATTTGGAAT ATTTGGCAAG AGTGGTATTT GATCAGGATG GATATTATTA CCCAGACAGC 
CTCGTGGGCA CAGACTCGCA CACTACCATG ATTGATGGCT TGGGCATTCT TGGTTGGGGT 
GTCGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CAATCAGTAT GGTGCTTCCT 
CAGGTGATTG GCTACAGGCT GATGGGGAAG CCCCACCCTC TGGTAACATC CACTGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGCCAG GTTGGGGTAG TGGGCAAATT TGTCGAGTTC 
TTCGGGCCTG GAGTAGCCCA GTTGTCCATT GCTGACCGAG CTACGATTGC TAACATGTGT 
CCAGAGTACG GAGCAACTGC TGCCTTTTTC CCAGTTGATG AAGTTAGTAT CACGTACCTG 
GTGCAAACAG GTCGTGATGA AGAAAAATTA AAGTATATTA AAAAATATCT TCAGGCTGTA 
GGAATGTTTC GAGATTTCAA TGACCCTTCT CAAGACCCAG ACTTCACCCA GGTTGTGGAA 
TTAGATTTGA AAACAGTAGT GCCTTGCTGT AGTGGACCCA AAAGGCCTCA GGACAAAGTT 
GCTGTGTCCG ACATGAAAAA GGACTTTGAG AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGATTCCAAG TTGCTCCTGA ACATCATAAT GACCATAAGA CCTTTATCTA TGATAACACT 
GAATTCACCC TTGCTCATGG TTCTGTGGTC ATTGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCGT CTGTGATGTT AGGGGCAGGA TTGTTAGCAA AGAAAGCTGT GGATGCTGGC 
CTGAACGTGA TGCCTTACAT CAAAACTAGC CTGTCTCCTG GGAGTGGCGT GGTCACCTAC 
TACCTACAAG AAAGCGGAGT CATGCCTTAT CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT TGGCAACAGT GGGCCTTTAC CTGAACCTGT GGTAGAAGCC 
ATCACACAGG GAGACCTTGT AGCTGTTGGA GTACTATCTG GAAACAGGAA TTTTGAAGGT 
CGAGTTCACC CCAACACCCG GGCCAACTAT TTAGCCTCTC CCCCCTTAGT AATAGCATAT 
GCAATTGCTG GAACCATCAG AATCGACTTT GAGAAAGAGC CATTGGGAGT AAATGCAAAG 
GGACAGCAGG TATTTCTGAA AGATATCTGG CCGACTAGAG ACGAGATCCA GGCAGTGGAG 
CGTCAGTATG TCATCCCGGG GATGTTTAAG GAAGTCTATC AGAAAATAGA GACTGTGAAT 
GAAAGCTGGA ATGCCTTAGC AACCCCATCA GATAAGCTGT TTTTCTGGAA TTCCAAATCT 
ACGTATATCA AATCACCACC ATTCTTTGAA AACCTGACTT TGGATCTTCA GCCCCCTAAA 
TCTATAGTGG ATGCCTATGT GCTGCTAAAT TTGGGAGATT CGGTAACAAC TGACCACATC 
TCCCCAGCTG GAAATATTGC AAGAAACAGT CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTAACTCCAC GAGAATTCAA CTCCTATGGC TCCCGCCGAG GTAATGACGC CGTCATGGCA 
CGGGGAACAT TTGCCAACAT TCGCTTGTTA AACAGATTTT TGAACAAGCA GGCACCACAG 
ACTATCCATC TGCCTTCTGG GGAAATCCTT GATGTGTTTG ATGCTGCTGA GCGGTACCAG 
CAGGCAGGCC TTCCCCTGAT CGTTCTGGCT GGCAAAGAGT ACGGTGCAGG CAGCTCCCGA 
GACTGGGCAG CTAAGGGCCC TTTCCTGCTG GGAATCAAAG CCGTCCTGGC CGAGAGCTAC 
GAGCGCATTC ACCGCAGTAA CCTGGTTGGG ATGGGTGTGA TCCCACTTGA ATATCTCCCT 
GGTGAGAATG CAGATGCCCT GGGGCTCACA GGGCAAGAAC GATACACTAT CATTATTCCA 
GAAAACCTCA AACCACAAAT GAAAGTCCAG GTCAAGCTGG ATACTGGCAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC TGATGTGGAG CTCACTTATT TCCTCAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACO1 ( NM_002197.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002188.1 Homo sapiens aconitase 1 (ACO1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17506
Clone ID Related Accession (Same CDS sequence)	NM_001362840.2 , XM_011517888.1 , NM_001278352.1 , NM_002197.2 , NM_001362840.1 , NM_002197.3 , XM_005251476.1 , XM_011517888.2
Accession Version	NM_002197.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-15
Organism	Homo sapiens(human)
Product	cytoplasmic aconitate hydratase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA409113.1 and BC018103.1. On Jan 27, 2008 this sequence version replaced NM_002197.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.5872.1, SRR1660807.122731.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 8041788 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCAACC CATTCGCACA CCTTGCTGAG CCATTGGATC CTGTACAACC AGGAAAGAAA 
TTCTTCAATT TGAATAAATT GGAGGATTCA AGATATGGGC GCTTACCATT TTCGATCAGA 
GTTCTTCTGG AAGCAGCCAT TCGGAATTGT GATGAGTTTT TGGTGAAGAA ACAGGATATT 
GAAAATATTC TACATTGGAA TGTCACGCAG CACAAGAACA TAGAAGTGCC ATTTAAGCCT 
GCTCGTGTCA TCCTGCAGGA CTTTACGGGT GTGCCCGCTG TGGTTGACTT TGCTGCAATG 
CGTGATGCTG TGAAAAAGTT AGGAGGAGAT CCAGAGAAAA TAAACCCTGT CTGCCCTGCT 
GATCTTGTAA TAGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAATT TGAAAGAAAT AGAGAGCGAT TTGAATTTTT AAAGTGGGGT 
TCCCAGGCTT TTCACAACAT GCGGATTATT CCCCCTGGCT CAGGAATCAT CCACCAGGTG 
AATTTGGAAT ATTTGGCAAG AGTGGTATTT GATCAGGATG GATATTATTA CCCAGACAGC 
CTCGTGGGCA CAGACTCGCA CACTACCATG ATTGATGGCT TGGGCATTCT TGGTTGGGGT 
GTCGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CAATCAGTAT GGTGCTTCCT 
CAGGTGATTG GCTACAGGCT GATGGGGAAG CCCCACCCTC TGGTAACATC CACTGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGCCAG GTTGGGGTAG TGGGCAAATT TGTCGAGTTC 
TTCGGGCCTG GAGTAGCCCA GTTGTCCATT GCTGACCGAG CTACGATTGC TAACATGTGT 
CCAGAGTACG GAGCAACTGC TGCCTTTTTC CCAGTTGATG AAGTTAGTAT CACGTACCTG 
GTGCAAACAG GTCGTGATGA AGAAAAATTA AAGTATATTA AAAAATATCT TCAGGCTGTA 
GGAATGTTTC GAGATTTCAA TGACCCTTCT CAAGACCCAG ACTTCACCCA GGTTGTGGAA 
TTAGATTTGA AAACAGTAGT GCCTTGCTGT AGTGGACCCA AAAGGCCTCA GGACAAAGTT 
GCTGTGTCCG ACATGAAAAA GGACTTTGAG AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGATTCCAAG TTGCTCCTGA ACATCATAAT GACCATAAGA CCTTTATCTA TGATAACACT 
GAATTCACCC TTGCTCATGG TTCTGTGGTC ATTGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCGT CTGTGATGTT AGGGGCAGGA TTGTTAGCAA AGAAAGCTGT GGATGCTGGC 
CTGAACGTGA TGCCTTACAT CAAAACTAGC CTGTCTCCTG GGAGTGGCGT GGTCACCTAC 
TACCTACAAG AAAGCGGAGT CATGCCTTAT CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT TGGCAACAGT GGGCCTTTAC CTGAACCTGT GGTAGAAGCC 
ATCACACAGG GAGACCTTGT AGCTGTTGGA GTACTATCTG GAAACAGGAA TTTTGAAGGT 
CGAGTTCACC CCAACACCCG GGCCAACTAT TTAGCCTCTC CCCCCTTAGT AATAGCATAT 
GCAATTGCTG GAACCATCAG AATCGACTTT GAGAAAGAGC CATTGGGAGT AAATGCAAAG 
GGACAGCAGG TATTTCTGAA AGATATCTGG CCGACTAGAG ACGAGATCCA GGCAGTGGAG 
CGTCAGTATG TCATCCCGGG GATGTTTAAG GAAGTCTATC AGAAAATAGA GACTGTGAAT 
GAAAGCTGGA ATGCCTTAGC AACCCCATCA GATAAGCTGT TTTTCTGGAA TTCCAAATCT 
ACGTATATCA AATCACCACC ATTCTTTGAA AACCTGACTT TGGATCTTCA GCCCCCTAAA 
TCTATAGTGG ATGCCTATGT GCTGCTAAAT TTGGGAGATT CGGTAACAAC TGACCACATC 
TCCCCAGCTG GAAATATTGC AAGAAACAGT CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTAACTCCAC GAGAATTCAA CTCCTATGGC TCCCGCCGAG GTAATGACGC CGTCATGGCA 
CGGGGAACAT TTGCCAACAT TCGCTTGTTA AACAGATTTT TGAACAAGCA GGCACCACAG 
ACTATCCATC TGCCTTCTGG GGAAATCCTT GATGTGTTTG ATGCTGCTGA GCGGTACCAG 
CAGGCAGGCC TTCCCCTGAT CGTTCTGGCT GGCAAAGAGT ACGGTGCAGG CAGCTCCCGA 
GACTGGGCAG CTAAGGGCCC TTTCCTGCTG GGAATCAAAG CCGTCCTGGC CGAGAGCTAC 
GAGCGCATTC ACCGCAGTAA CCTGGTTGGG ATGGGTGTGA TCCCACTTGA ATATCTCCCT 
GGTGAGAATG CAGATGCCCT GGGGCTCACA GGGCAAGAAC GATACACTAT CATTATTCCA 
GAAAACCTCA AACCACAAAT GAAAGTCCAG GTCAAGCTGG ATACTGGCAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC TGATGTGGAG CTCACTTATT TCCTCAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002188.1
CDS	156..2825
Translation	MSNPFAHLAEPLDPVQPGKKFFNLNKLEDSRYGRLPFSIRVLLEAAIRNCDEFLVKKQDIENILHWNVTQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNRERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGILGWGVGGIEAEAVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSITYLVQTGRDEEKLKYIKKYLQAVGMFRDFNDPSQDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPEHHNDHKTFIYDNTEFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLNVMPYIKTSLSPGSGVVTYYLQESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMIRKMAK

Target ORF information:

RefSeq Version	NM_002197.2
Organism	Homo sapiens(human)
Definition	Homo sapiens aconitase 1 (ACO1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002197.2

ORF Insert Sequence:

ATGAGCAACC CATTCGCACA CCTTGCTGAG CCATTGGATC CTGTACAACC AGGAAAGAAA 
TTCTTCAATT TGAATAAATT GGAGGATTCA AGATATGGGC GCTTACCATT TTCGATCAGA 
GTTCTTCTGG AAGCAGCCAT TCGGAATTGT GATGAGTTTT TGGTGAAGAA ACAGGATATT 
GAAAATATTC TACATTGGAA TGTCACGCAG CACAAGAACA TAGAAGTGCC ATTTAAGCCT 
GCTCGTGTCA TCCTGCAGGA CTTTACGGGT GTGCCCGCTG TGGTTGACTT TGCTGCAATG 
CGTGATGCTG TGAAAAAGTT AGGAGGAGAT CCAGAGAAAA TAAACCCTGT CTGCCCTGCT 
GATCTTGTAA TAGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAATT TGAAAGAAAT AGAGAGCGAT TTGAATTTTT AAAGTGGGGT 
TCCCAGGCTT TTCACAACAT GCGGATTATT CCCCCTGGCT CAGGAATCAT CCACCAGGTG 
AATTTGGAAT ATTTGGCAAG AGTGGTATTT GATCAGGATG GATATTATTA CCCAGACAGC 
CTCGTGGGCA CAGACTCGCA CACTACCATG ATTGATGGCT TGGGCATTCT TGGTTGGGGT 
GTCGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CAATCAGTAT GGTGCTTCCT 
CAGGTGATTG GCTACAGGCT GATGGGGAAG CCCCACCCTC TGGTAACATC CACTGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGCCAG GTTGGGGTAG TGGGCAAATT TGTCGAGTTC 
TTCGGGCCTG GAGTAGCCCA GTTGTCCATT GCTGACCGAG CTACGATTGC TAACATGTGT 
CCAGAGTACG GAGCAACTGC TGCCTTTTTC CCAGTTGATG AAGTTAGTAT CACGTACCTG 
GTGCAAACAG GTCGTGATGA AGAAAAATTA AAGTATATTA AAAAATATCT TCAGGCTGTA 
GGAATGTTTC GAGATTTCAA TGACCCTTCT CAAGACCCAG ACTTCACCCA GGTTGTGGAA 
TTAGATTTGA AAACAGTAGT GCCTTGCTGT AGTGGACCCA AAAGGCCTCA GGACAAAGTT 
GCTGTGTCCG ACATGAAAAA GGACTTTGAG AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGATTCCAAG TTGCTCCTGA ACATCATAAT GACCATAAGA CCTTTATCTA TGATAACACT 
GAATTCACCC TTGCTCATGG TTCTGTGGTC ATTGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCGT CTGTGATGTT AGGGGCAGGA TTGTTAGCAA AGAAAGCTGT GGATGCTGGC 
CTGAACGTGA TGCCTTACAT CAAAACTAGC CTGTCTCCTG GGAGTGGCGT GGTCACCTAC 
TACCTACAAG AAAGCGGAGT CATGCCTTAT CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT TGGCAACAGT GGGCCTTTAC CTGAACCTGT GGTAGAAGCC 
ATCACACAGG GAGACCTTGT AGCTGTTGGA GTACTATCTG GAAACAGGAA TTTTGAAGGT 
CGAGTTCACC CCAACACCCG GGCCAACTAT TTAGCCTCTC CCCCCTTAGT AATAGCATAT 
GCAATTGCTG GAACCATCAG AATCGACTTT GAGAAAGAGC CATTGGGAGT AAATGCAAAG 
GGACAGCAGG TATTTCTGAA AGATATCTGG CCGACTAGAG ACGAGATCCA GGCAGTGGAG 
CGTCAGTATG TCATCCCGGG GATGTTTAAG GAAGTCTATC AGAAAATAGA GACTGTGAAT 
GAAAGCTGGA ATGCCTTAGC AACCCCATCA GATAAGCTGT TTTTCTGGAA TTCCAAATCT 
ACGTATATCA AATCACCACC ATTCTTTGAA AACCTGACTT TGGATCTTCA GCCCCCTAAA 
TCTATAGTGG ATGCCTATGT GCTGCTAAAT TTGGGAGATT CGGTAACAAC TGACCACATC 
TCCCCAGCTG GAAATATTGC AAGAAACAGT CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTAACTCCAC GAGAATTCAA CTCCTATGGC TCCCGCCGAG GTAATGACGC CGTCATGGCA 
CGGGGAACAT TTGCCAACAT TCGCTTGTTA AACAGATTTT TGAACAAGCA GGCACCACAG 
ACTATCCATC TGCCTTCTGG GGAAATCCTT GATGTGTTTG ATGCTGCTGA GCGGTACCAG 
CAGGCAGGCC TTCCCCTGAT CGTTCTGGCT GGCAAAGAGT ACGGTGCAGG CAGCTCCCGA 
GACTGGGCAG CTAAGGGCCC TTTCCTGCTG GGAATCAAAG CCGTCCTGGC CGAGAGCTAC 
GAGCGCATTC ACCGCAGTAA CCTGGTTGGG ATGGGTGTGA TCCCACTTGA ATATCTCCCT 
GGTGAGAATG CAGATGCCCT GGGGCTCACA GGGCAAGAAC GATACACTAT CATTATTCCA 
GAAAACCTCA AACCACAAAT GAAAGTCCAG GTCAAGCTGG ATACTGGCAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC TGATGTGGAG CTCACTTATT TCCTCAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACO1 ( NM_001362840.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001349769.1 Homo sapiens aconitase 1 (ACO1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu17506
Clone ID Related Accession (Same CDS sequence)	NM_001362840.2 , XM_011517888.1 , NM_001278352.1 , NM_002197.2 , NM_001362840.1 , NM_002197.3 , XM_005251476.1 , XM_011517888.2
Accession Version	NM_001362840.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	cytoplasmic aconitate hydratase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA780353.1, BP287929.1, AF261088.1 and BC018103.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.124049.1, SRR1803615.264975.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 8041788 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCAACC CATTCGCACA CCTTGCTGAG CCATTGGATC CTGTACAACC AGGAAAGAAA 
TTCTTCAATT TGAATAAATT GGAGGATTCA AGATATGGGC GCTTACCATT TTCGATCAGA 
GTTCTTCTGG AAGCAGCCAT TCGGAATTGT GATGAGTTTT TGGTGAAGAA ACAGGATATT 
GAAAATATTC TACATTGGAA TGTCACGCAG CACAAGAACA TAGAAGTGCC ATTTAAGCCT 
GCTCGTGTCA TCCTGCAGGA CTTTACGGGT GTGCCCGCTG TGGTTGACTT TGCTGCAATG 
CGTGATGCTG TGAAAAAGTT AGGAGGAGAT CCAGAGAAAA TAAACCCTGT CTGCCCTGCT 
GATCTTGTAA TAGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAATT TGAAAGAAAT AGAGAGCGAT TTGAATTTTT AAAGTGGGGT 
TCCCAGGCTT TTCACAACAT GCGGATTATT CCCCCTGGCT CAGGAATCAT CCACCAGGTG 
AATTTGGAAT ATTTGGCAAG AGTGGTATTT GATCAGGATG GATATTATTA CCCAGACAGC 
CTCGTGGGCA CAGACTCGCA CACTACCATG ATTGATGGCT TGGGCATTCT TGGTTGGGGT 
GTCGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CAATCAGTAT GGTGCTTCCT 
CAGGTGATTG GCTACAGGCT GATGGGGAAG CCCCACCCTC TGGTAACATC CACTGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGCCAG GTTGGGGTAG TGGGCAAATT TGTCGAGTTC 
TTCGGGCCTG GAGTAGCCCA GTTGTCCATT GCTGACCGAG CTACGATTGC TAACATGTGT 
CCAGAGTACG GAGCAACTGC TGCCTTTTTC CCAGTTGATG AAGTTAGTAT CACGTACCTG 
GTGCAAACAG GTCGTGATGA AGAAAAATTA AAGTATATTA AAAAATATCT TCAGGCTGTA 
GGAATGTTTC GAGATTTCAA TGACCCTTCT CAAGACCCAG ACTTCACCCA GGTTGTGGAA 
TTAGATTTGA AAACAGTAGT GCCTTGCTGT AGTGGACCCA AAAGGCCTCA GGACAAAGTT 
GCTGTGTCCG ACATGAAAAA GGACTTTGAG AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGATTCCAAG TTGCTCCTGA ACATCATAAT GACCATAAGA CCTTTATCTA TGATAACACT 
GAATTCACCC TTGCTCATGG TTCTGTGGTC ATTGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCGT CTGTGATGTT AGGGGCAGGA TTGTTAGCAA AGAAAGCTGT GGATGCTGGC 
CTGAACGTGA TGCCTTACAT CAAAACTAGC CTGTCTCCTG GGAGTGGCGT GGTCACCTAC 
TACCTACAAG AAAGCGGAGT CATGCCTTAT CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT TGGCAACAGT GGGCCTTTAC CTGAACCTGT GGTAGAAGCC 
ATCACACAGG GAGACCTTGT AGCTGTTGGA GTACTATCTG GAAACAGGAA TTTTGAAGGT 
CGAGTTCACC CCAACACCCG GGCCAACTAT TTAGCCTCTC CCCCCTTAGT AATAGCATAT 
GCAATTGCTG GAACCATCAG AATCGACTTT GAGAAAGAGC CATTGGGAGT AAATGCAAAG 
GGACAGCAGG TATTTCTGAA AGATATCTGG CCGACTAGAG ACGAGATCCA GGCAGTGGAG 
CGTCAGTATG TCATCCCGGG GATGTTTAAG GAAGTCTATC AGAAAATAGA GACTGTGAAT 
GAAAGCTGGA ATGCCTTAGC AACCCCATCA GATAAGCTGT TTTTCTGGAA TTCCAAATCT 
ACGTATATCA AATCACCACC ATTCTTTGAA AACCTGACTT TGGATCTTCA GCCCCCTAAA 
TCTATAGTGG ATGCCTATGT GCTGCTAAAT TTGGGAGATT CGGTAACAAC TGACCACATC 
TCCCCAGCTG GAAATATTGC AAGAAACAGT CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTAACTCCAC GAGAATTCAA CTCCTATGGC TCCCGCCGAG GTAATGACGC CGTCATGGCA 
CGGGGAACAT TTGCCAACAT TCGCTTGTTA AACAGATTTT TGAACAAGCA GGCACCACAG 
ACTATCCATC TGCCTTCTGG GGAAATCCTT GATGTGTTTG ATGCTGCTGA GCGGTACCAG 
CAGGCAGGCC TTCCCCTGAT CGTTCTGGCT GGCAAAGAGT ACGGTGCAGG CAGCTCCCGA 
GACTGGGCAG CTAAGGGCCC TTTCCTGCTG GGAATCAAAG CCGTCCTGGC CGAGAGCTAC 
GAGCGCATTC ACCGCAGTAA CCTGGTTGGG ATGGGTGTGA TCCCACTTGA ATATCTCCCT 
GGTGAGAATG CAGATGCCCT GGGGCTCACA GGGCAAGAAC GATACACTAT CATTATTCCA 
GAAAACCTCA AACCACAAAT GAAAGTCCAG GTCAAGCTGG ATACTGGCAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC TGATGTGGAG CTCACTTATT TCCTCAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349769.1
CDS	224..2893
Translation	MSNPFAHLAEPLDPVQPGKKFFNLNKLEDSRYGRLPFSIRVLLEAAIRNCDEFLVKKQDIENILHWNVTQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNRERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGILGWGVGGIEAEAVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSITYLVQTGRDEEKLKYIKKYLQAVGMFRDFNDPSQDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPEHHNDHKTFIYDNTEFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLNVMPYIKTSLSPGSGVVTYYLQESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMIRKMAK

Target ORF information:

RefSeq Version	NM_001362840.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aconitase 1 (ACO1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362840.1

ORF Insert Sequence:

ATGAGCAACC CATTCGCACA CCTTGCTGAG CCATTGGATC CTGTACAACC AGGAAAGAAA 
TTCTTCAATT TGAATAAATT GGAGGATTCA AGATATGGGC GCTTACCATT TTCGATCAGA 
GTTCTTCTGG AAGCAGCCAT TCGGAATTGT GATGAGTTTT TGGTGAAGAA ACAGGATATT 
GAAAATATTC TACATTGGAA TGTCACGCAG CACAAGAACA TAGAAGTGCC ATTTAAGCCT 
GCTCGTGTCA TCCTGCAGGA CTTTACGGGT GTGCCCGCTG TGGTTGACTT TGCTGCAATG 
CGTGATGCTG TGAAAAAGTT AGGAGGAGAT CCAGAGAAAA TAAACCCTGT CTGCCCTGCT 
GATCTTGTAA TAGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAATT TGAAAGAAAT AGAGAGCGAT TTGAATTTTT AAAGTGGGGT 
TCCCAGGCTT TTCACAACAT GCGGATTATT CCCCCTGGCT CAGGAATCAT CCACCAGGTG 
AATTTGGAAT ATTTGGCAAG AGTGGTATTT GATCAGGATG GATATTATTA CCCAGACAGC 
CTCGTGGGCA CAGACTCGCA CACTACCATG ATTGATGGCT TGGGCATTCT TGGTTGGGGT 
GTCGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CAATCAGTAT GGTGCTTCCT 
CAGGTGATTG GCTACAGGCT GATGGGGAAG CCCCACCCTC TGGTAACATC CACTGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGCCAG GTTGGGGTAG TGGGCAAATT TGTCGAGTTC 
TTCGGGCCTG GAGTAGCCCA GTTGTCCATT GCTGACCGAG CTACGATTGC TAACATGTGT 
CCAGAGTACG GAGCAACTGC TGCCTTTTTC CCAGTTGATG AAGTTAGTAT CACGTACCTG 
GTGCAAACAG GTCGTGATGA AGAAAAATTA AAGTATATTA AAAAATATCT TCAGGCTGTA 
GGAATGTTTC GAGATTTCAA TGACCCTTCT CAAGACCCAG ACTTCACCCA GGTTGTGGAA 
TTAGATTTGA AAACAGTAGT GCCTTGCTGT AGTGGACCCA AAAGGCCTCA GGACAAAGTT 
GCTGTGTCCG ACATGAAAAA GGACTTTGAG AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGATTCCAAG TTGCTCCTGA ACATCATAAT GACCATAAGA CCTTTATCTA TGATAACACT 
GAATTCACCC TTGCTCATGG TTCTGTGGTC ATTGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCGT CTGTGATGTT AGGGGCAGGA TTGTTAGCAA AGAAAGCTGT GGATGCTGGC 
CTGAACGTGA TGCCTTACAT CAAAACTAGC CTGTCTCCTG GGAGTGGCGT GGTCACCTAC 
TACCTACAAG AAAGCGGAGT CATGCCTTAT CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT TGGCAACAGT GGGCCTTTAC CTGAACCTGT GGTAGAAGCC 
ATCACACAGG GAGACCTTGT AGCTGTTGGA GTACTATCTG GAAACAGGAA TTTTGAAGGT 
CGAGTTCACC CCAACACCCG GGCCAACTAT TTAGCCTCTC CCCCCTTAGT AATAGCATAT 
GCAATTGCTG GAACCATCAG AATCGACTTT GAGAAAGAGC CATTGGGAGT AAATGCAAAG 
GGACAGCAGG TATTTCTGAA AGATATCTGG CCGACTAGAG ACGAGATCCA GGCAGTGGAG 
CGTCAGTATG TCATCCCGGG GATGTTTAAG GAAGTCTATC AGAAAATAGA GACTGTGAAT 
GAAAGCTGGA ATGCCTTAGC AACCCCATCA GATAAGCTGT TTTTCTGGAA TTCCAAATCT 
ACGTATATCA AATCACCACC ATTCTTTGAA AACCTGACTT TGGATCTTCA GCCCCCTAAA 
TCTATAGTGG ATGCCTATGT GCTGCTAAAT TTGGGAGATT CGGTAACAAC TGACCACATC 
TCCCCAGCTG GAAATATTGC AAGAAACAGT CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTAACTCCAC GAGAATTCAA CTCCTATGGC TCCCGCCGAG GTAATGACGC CGTCATGGCA 
CGGGGAACAT TTGCCAACAT TCGCTTGTTA AACAGATTTT TGAACAAGCA GGCACCACAG 
ACTATCCATC TGCCTTCTGG GGAAATCCTT GATGTGTTTG ATGCTGCTGA GCGGTACCAG 
CAGGCAGGCC TTCCCCTGAT CGTTCTGGCT GGCAAAGAGT ACGGTGCAGG CAGCTCCCGA 
GACTGGGCAG CTAAGGGCCC TTTCCTGCTG GGAATCAAAG CCGTCCTGGC CGAGAGCTAC 
GAGCGCATTC ACCGCAGTAA CCTGGTTGGG ATGGGTGTGA TCCCACTTGA ATATCTCCCT 
GGTGAGAATG CAGATGCCCT GGGGCTCACA GGGCAAGAAC GATACACTAT CATTATTCCA 
GAAAACCTCA AACCACAAAT GAAAGTCCAG GTCAAGCTGG ATACTGGCAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC TGATGTGGAG CTCACTTATT TCCTCAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACO1 ( NM_002197.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002188.1 Homo sapiens aconitase 1 (ACO1), transcript variant 2, mRNA.

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CloneID	OHu17506
Clone ID Related Accession (Same CDS sequence)	NM_001362840.2 , XM_011517888.1 , NM_001278352.1 , NM_002197.2 , NM_001362840.1 , NM_002197.3 , XM_005251476.1 , XM_011517888.2
Accession Version	NM_002197.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	cytoplasmic aconitate hydratase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA409113.1, BC018103.1 and AL161783.20. On Nov 23, 2018 this sequence version replaced NM_002197.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.88108.1, SRR1803615.81823.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000309951.8/ ENSP00000309477.5 multifunctional gene product(s) :: PMID: 8041788 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCAACC CATTCGCACA CCTTGCTGAG CCATTGGATC CTGTACAACC AGGAAAGAAA 
TTCTTCAATT TGAATAAATT GGAGGATTCA AGATATGGGC GCTTACCATT TTCGATCAGA 
GTTCTTCTGG AAGCAGCCAT TCGGAATTGT GATGAGTTTT TGGTGAAGAA ACAGGATATT 
GAAAATATTC TACATTGGAA TGTCACGCAG CACAAGAACA TAGAAGTGCC ATTTAAGCCT 
GCTCGTGTCA TCCTGCAGGA CTTTACGGGT GTGCCCGCTG TGGTTGACTT TGCTGCAATG 
CGTGATGCTG TGAAAAAGTT AGGAGGAGAT CCAGAGAAAA TAAACCCTGT CTGCCCTGCT 
GATCTTGTAA TAGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAATT TGAAAGAAAT AGAGAGCGAT TTGAATTTTT AAAGTGGGGT 
TCCCAGGCTT TTCACAACAT GCGGATTATT CCCCCTGGCT CAGGAATCAT CCACCAGGTG 
AATTTGGAAT ATTTGGCAAG AGTGGTATTT GATCAGGATG GATATTATTA CCCAGACAGC 
CTCGTGGGCA CAGACTCGCA CACTACCATG ATTGATGGCT TGGGCATTCT TGGTTGGGGT 
GTCGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CAATCAGTAT GGTGCTTCCT 
CAGGTGATTG GCTACAGGCT GATGGGGAAG CCCCACCCTC TGGTAACATC CACTGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGCCAG GTTGGGGTAG TGGGCAAATT TGTCGAGTTC 
TTCGGGCCTG GAGTAGCCCA GTTGTCCATT GCTGACCGAG CTACGATTGC TAACATGTGT 
CCAGAGTACG GAGCAACTGC TGCCTTTTTC CCAGTTGATG AAGTTAGTAT CACGTACCTG 
GTGCAAACAG GTCGTGATGA AGAAAAATTA AAGTATATTA AAAAATATCT TCAGGCTGTA 
GGAATGTTTC GAGATTTCAA TGACCCTTCT CAAGACCCAG ACTTCACCCA GGTTGTGGAA 
TTAGATTTGA AAACAGTAGT GCCTTGCTGT AGTGGACCCA AAAGGCCTCA GGACAAAGTT 
GCTGTGTCCG ACATGAAAAA GGACTTTGAG AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGATTCCAAG TTGCTCCTGA ACATCATAAT GACCATAAGA CCTTTATCTA TGATAACACT 
GAATTCACCC TTGCTCATGG TTCTGTGGTC ATTGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCGT CTGTGATGTT AGGGGCAGGA TTGTTAGCAA AGAAAGCTGT GGATGCTGGC 
CTGAACGTGA TGCCTTACAT CAAAACTAGC CTGTCTCCTG GGAGTGGCGT GGTCACCTAC 
TACCTACAAG AAAGCGGAGT CATGCCTTAT CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT TGGCAACAGT GGGCCTTTAC CTGAACCTGT GGTAGAAGCC 
ATCACACAGG GAGACCTTGT AGCTGTTGGA GTACTATCTG GAAACAGGAA TTTTGAAGGT 
CGAGTTCACC CCAACACCCG GGCCAACTAT TTAGCCTCTC CCCCCTTAGT AATAGCATAT 
GCAATTGCTG GAACCATCAG AATCGACTTT GAGAAAGAGC CATTGGGAGT AAATGCAAAG 
GGACAGCAGG TATTTCTGAA AGATATCTGG CCGACTAGAG ACGAGATCCA GGCAGTGGAG 
CGTCAGTATG TCATCCCGGG GATGTTTAAG GAAGTCTATC AGAAAATAGA GACTGTGAAT 
GAAAGCTGGA ATGCCTTAGC AACCCCATCA GATAAGCTGT TTTTCTGGAA TTCCAAATCT 
ACGTATATCA AATCACCACC ATTCTTTGAA AACCTGACTT TGGATCTTCA GCCCCCTAAA 
TCTATAGTGG ATGCCTATGT GCTGCTAAAT TTGGGAGATT CGGTAACAAC TGACCACATC 
TCCCCAGCTG GAAATATTGC AAGAAACAGT CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTAACTCCAC GAGAATTCAA CTCCTATGGC TCCCGCCGAG GTAATGACGC CGTCATGGCA 
CGGGGAACAT TTGCCAACAT TCGCTTGTTA AACAGATTTT TGAACAAGCA GGCACCACAG 
ACTATCCATC TGCCTTCTGG GGAAATCCTT GATGTGTTTG ATGCTGCTGA GCGGTACCAG 
CAGGCAGGCC TTCCCCTGAT CGTTCTGGCT GGCAAAGAGT ACGGTGCAGG CAGCTCCCGA 
GACTGGGCAG CTAAGGGCCC TTTCCTGCTG GGAATCAAAG CCGTCCTGGC CGAGAGCTAC 
GAGCGCATTC ACCGCAGTAA CCTGGTTGGG ATGGGTGTGA TCCCACTTGA ATATCTCCCT 
GGTGAGAATG CAGATGCCCT GGGGCTCACA GGGCAAGAAC GATACACTAT CATTATTCCA 
GAAAACCTCA AACCACAAAT GAAAGTCCAG GTCAAGCTGG ATACTGGCAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC TGATGTGGAG CTCACTTATT TCCTCAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002188.1
CDS	116..2785
Translation	MSNPFAHLAEPLDPVQPGKKFFNLNKLEDSRYGRLPFSIRVLLEAAIRNCDEFLVKKQDIENILHWNVTQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNRERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGILGWGVGGIEAEAVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSITYLVQTGRDEEKLKYIKKYLQAVGMFRDFNDPSQDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPEHHNDHKTFIYDNTEFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLNVMPYIKTSLSPGSGVVTYYLQESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMIRKMAK

Target ORF information:

RefSeq Version	NM_002197.3
Organism	Homo sapiens(human)
Definition	Homo sapiens aconitase 1 (ACO1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002197.3

ORF Insert Sequence:

ATGAGCAACC CATTCGCACA CCTTGCTGAG CCATTGGATC CTGTACAACC AGGAAAGAAA 
TTCTTCAATT TGAATAAATT GGAGGATTCA AGATATGGGC GCTTACCATT TTCGATCAGA 
GTTCTTCTGG AAGCAGCCAT TCGGAATTGT GATGAGTTTT TGGTGAAGAA ACAGGATATT 
GAAAATATTC TACATTGGAA TGTCACGCAG CACAAGAACA TAGAAGTGCC ATTTAAGCCT 
GCTCGTGTCA TCCTGCAGGA CTTTACGGGT GTGCCCGCTG TGGTTGACTT TGCTGCAATG 
CGTGATGCTG TGAAAAAGTT AGGAGGAGAT CCAGAGAAAA TAAACCCTGT CTGCCCTGCT 
GATCTTGTAA TAGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAATT TGAAAGAAAT AGAGAGCGAT TTGAATTTTT AAAGTGGGGT 
TCCCAGGCTT TTCACAACAT GCGGATTATT CCCCCTGGCT CAGGAATCAT CCACCAGGTG 
AATTTGGAAT ATTTGGCAAG AGTGGTATTT GATCAGGATG GATATTATTA CCCAGACAGC 
CTCGTGGGCA CAGACTCGCA CACTACCATG ATTGATGGCT TGGGCATTCT TGGTTGGGGT 
GTCGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CAATCAGTAT GGTGCTTCCT 
CAGGTGATTG GCTACAGGCT GATGGGGAAG CCCCACCCTC TGGTAACATC CACTGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGCCAG GTTGGGGTAG TGGGCAAATT TGTCGAGTTC 
TTCGGGCCTG GAGTAGCCCA GTTGTCCATT GCTGACCGAG CTACGATTGC TAACATGTGT 
CCAGAGTACG GAGCAACTGC TGCCTTTTTC CCAGTTGATG AAGTTAGTAT CACGTACCTG 
GTGCAAACAG GTCGTGATGA AGAAAAATTA AAGTATATTA AAAAATATCT TCAGGCTGTA 
GGAATGTTTC GAGATTTCAA TGACCCTTCT CAAGACCCAG ACTTCACCCA GGTTGTGGAA 
TTAGATTTGA AAACAGTAGT GCCTTGCTGT AGTGGACCCA AAAGGCCTCA GGACAAAGTT 
GCTGTGTCCG ACATGAAAAA GGACTTTGAG AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGATTCCAAG TTGCTCCTGA ACATCATAAT GACCATAAGA CCTTTATCTA TGATAACACT 
GAATTCACCC TTGCTCATGG TTCTGTGGTC ATTGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCGT CTGTGATGTT AGGGGCAGGA TTGTTAGCAA AGAAAGCTGT GGATGCTGGC 
CTGAACGTGA TGCCTTACAT CAAAACTAGC CTGTCTCCTG GGAGTGGCGT GGTCACCTAC 
TACCTACAAG AAAGCGGAGT CATGCCTTAT CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT TGGCAACAGT GGGCCTTTAC CTGAACCTGT GGTAGAAGCC 
ATCACACAGG GAGACCTTGT AGCTGTTGGA GTACTATCTG GAAACAGGAA TTTTGAAGGT 
CGAGTTCACC CCAACACCCG GGCCAACTAT TTAGCCTCTC CCCCCTTAGT AATAGCATAT 
GCAATTGCTG GAACCATCAG AATCGACTTT GAGAAAGAGC CATTGGGAGT AAATGCAAAG 
GGACAGCAGG TATTTCTGAA AGATATCTGG CCGACTAGAG ACGAGATCCA GGCAGTGGAG 
CGTCAGTATG TCATCCCGGG GATGTTTAAG GAAGTCTATC AGAAAATAGA GACTGTGAAT 
GAAAGCTGGA ATGCCTTAGC AACCCCATCA GATAAGCTGT TTTTCTGGAA TTCCAAATCT 
ACGTATATCA AATCACCACC ATTCTTTGAA AACCTGACTT TGGATCTTCA GCCCCCTAAA 
TCTATAGTGG ATGCCTATGT GCTGCTAAAT TTGGGAGATT CGGTAACAAC TGACCACATC 
TCCCCAGCTG GAAATATTGC AAGAAACAGT CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTAACTCCAC GAGAATTCAA CTCCTATGGC TCCCGCCGAG GTAATGACGC CGTCATGGCA 
CGGGGAACAT TTGCCAACAT TCGCTTGTTA AACAGATTTT TGAACAAGCA GGCACCACAG 
ACTATCCATC TGCCTTCTGG GGAAATCCTT GATGTGTTTG ATGCTGCTGA GCGGTACCAG 
CAGGCAGGCC TTCCCCTGAT CGTTCTGGCT GGCAAAGAGT ACGGTGCAGG CAGCTCCCGA 
GACTGGGCAG CTAAGGGCCC TTTCCTGCTG GGAATCAAAG CCGTCCTGGC CGAGAGCTAC 
GAGCGCATTC ACCGCAGTAA CCTGGTTGGG ATGGGTGTGA TCCCACTTGA ATATCTCCCT 
GGTGAGAATG CAGATGCCCT GGGGCTCACA GGGCAAGAAC GATACACTAT CATTATTCCA 
GAAAACCTCA AACCACAAAT GAAAGTCCAG GTCAAGCTGG ATACTGGCAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC TGATGTGGAG CTCACTTATT TCCTCAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACO1 ( XM_011517888.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516190.1 Homo sapiens aconitase 1 (ACO1), transcript variant X2, mRNA.

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CloneID	OHu17506
Clone ID Related Accession (Same CDS sequence)	NM_001362840.2 , XM_011517888.1 , NM_001278352.1 , NM_002197.2 , NM_001362840.1 , NM_002197.3 , XM_005251476.1 , XM_011517888.2
Accession Version	XM_011517888.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cytoplasmic aconitate hydratase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAACC CATTCGCACA CCTTGCTGAG CCATTGGATC CTGTACAACC AGGAAAGAAA 
TTCTTCAATT TGAATAAATT GGAGGATTCA AGATATGGGC GCTTACCATT TTCGATCAGA 
GTTCTTCTGG AAGCAGCCAT TCGGAATTGT GATGAGTTTT TGGTGAAGAA ACAGGATATT 
GAAAATATTC TACATTGGAA TGTCACGCAG CACAAGAACA TAGAAGTGCC ATTTAAGCCT 
GCTCGTGTCA TCCTGCAGGA CTTTACGGGT GTGCCCGCTG TGGTTGACTT TGCTGCAATG 
CGTGATGCTG TGAAAAAGTT AGGAGGAGAT CCAGAGAAAA TAAACCCTGT CTGCCCTGCT 
GATCTTGTAA TAGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAATT TGAAAGAAAT AGAGAGCGAT TTGAATTTTT AAAGTGGGGT 
TCCCAGGCTT TTCACAACAT GCGGATTATT CCCCCTGGCT CAGGAATCAT CCACCAGGTG 
AATTTGGAAT ATTTGGCAAG AGTGGTATTT GATCAGGATG GATATTATTA CCCAGACAGC 
CTCGTGGGCA CAGACTCGCA CACTACCATG ATTGATGGCT TGGGCATTCT TGGTTGGGGT 
GTCGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CAATCAGTAT GGTGCTTCCT 
CAGGTGATTG GCTACAGGCT GATGGGGAAG CCCCACCCTC TGGTAACATC CACTGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGCCAG GTTGGGGTAG TGGGCAAATT TGTCGAGTTC 
TTCGGGCCTG GAGTAGCCCA GTTGTCCATT GCTGACCGAG CTACGATTGC TAACATGTGT 
CCAGAGTACG GAGCAACTGC TGCCTTTTTC CCAGTTGATG AAGTTAGTAT CACGTACCTG 
GTGCAAACAG GTCGTGATGA AGAAAAATTA AAGTATATTA AAAAATATCT TCAGGCTGTA 
GGAATGTTTC GAGATTTCAA TGACCCTTCT CAAGACCCAG ACTTCACCCA GGTTGTGGAA 
TTAGATTTGA AAACAGTAGT GCCTTGCTGT AGTGGACCCA AAAGGCCTCA GGACAAAGTT 
GCTGTGTCCG ACATGAAAAA GGACTTTGAG AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGATTCCAAG TTGCTCCTGA ACATCATAAT GACCATAAGA CCTTTATCTA TGATAACACT 
GAATTCACCC TTGCTCATGG TTCTGTGGTC ATTGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCGT CTGTGATGTT AGGGGCAGGA TTGTTAGCAA AGAAAGCTGT GGATGCTGGC 
CTGAACGTGA TGCCTTACAT CAAAACTAGC CTGTCTCCTG GGAGTGGCGT GGTCACCTAC 
TACCTACAAG AAAGCGGAGT CATGCCTTAT CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT TGGCAACAGT GGGCCTTTAC CTGAACCTGT GGTAGAAGCC 
ATCACACAGG GAGACCTTGT AGCTGTTGGA GTACTATCTG GAAACAGGAA TTTTGAAGGT 
CGAGTTCACC CCAACACCCG GGCCAACTAT TTAGCCTCTC CCCCCTTAGT AATAGCATAT 
GCAATTGCTG GAACCATCAG AATCGACTTT GAGAAAGAGC CATTGGGAGT AAATGCAAAG 
GGACAGCAGG TATTTCTGAA AGATATCTGG CCGACTAGAG ACGAGATCCA GGCAGTGGAG 
CGTCAGTATG TCATCCCGGG GATGTTTAAG GAAGTCTATC AGAAAATAGA GACTGTGAAT 
GAAAGCTGGA ATGCCTTAGC AACCCCATCA GATAAGCTGT TTTTCTGGAA TTCCAAATCT 
ACGTATATCA AATCACCACC ATTCTTTGAA AACCTGACTT TGGATCTTCA GCCCCCTAAA 
TCTATAGTGG ATGCCTATGT GCTGCTAAAT TTGGGAGATT CGGTAACAAC TGACCACATC 
TCCCCAGCTG GAAATATTGC AAGAAACAGT CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTAACTCCAC GAGAATTCAA CTCCTATGGC TCCCGCCGAG GTAATGACGC CGTCATGGCA 
CGGGGAACAT TTGCCAACAT TCGCTTGTTA AACAGATTTT TGAACAAGCA GGCACCACAG 
ACTATCCATC TGCCTTCTGG GGAAATCCTT GATGTGTTTG ATGCTGCTGA GCGGTACCAG 
CAGGCAGGCC TTCCCCTGAT CGTTCTGGCT GGCAAAGAGT ACGGTGCAGG CAGCTCCCGA 
GACTGGGCAG CTAAGGGCCC TTTCCTGCTG GGAATCAAAG CCGTCCTGGC CGAGAGCTAC 
GAGCGCATTC ACCGCAGTAA CCTGGTTGGG ATGGGTGTGA TCCCACTTGA ATATCTCCCT 
GGTGAGAATG CAGATGCCCT GGGGCTCACA GGGCAAGAAC GATACACTAT CATTATTCCA 
GAAAACCTCA AACCACAAAT GAAAGTCCAG GTCAAGCTGG ATACTGGCAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC TGATGTGGAG CTCACTTATT TCCTCAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516190.1
CDS	78..2747
Translation	MSNPFAHLAEPLDPVQPGKKFFNLNKLEDSRYGRLPFSIRVLLEAAIRNCDEFLVKKQDIENILHWNVTQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNRERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGILGWGVGGIEAEAVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSITYLVQTGRDEEKLKYIKKYLQAVGMFRDFNDPSQDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPEHHNDHKTFIYDNTEFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLNVMPYIKTSLSPGSGVVTYYLQESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMIRKMAK

Target ORF information:

RefSeq Version	XM_011517888.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aconitase 1 (ACO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011517888.1

ORF Insert Sequence:

ATGAGCAACC CATTCGCACA CCTTGCTGAG CCATTGGATC CTGTACAACC AGGAAAGAAA 
TTCTTCAATT TGAATAAATT GGAGGATTCA AGATATGGGC GCTTACCATT TTCGATCAGA 
GTTCTTCTGG AAGCAGCCAT TCGGAATTGT GATGAGTTTT TGGTGAAGAA ACAGGATATT 
GAAAATATTC TACATTGGAA TGTCACGCAG CACAAGAACA TAGAAGTGCC ATTTAAGCCT 
GCTCGTGTCA TCCTGCAGGA CTTTACGGGT GTGCCCGCTG TGGTTGACTT TGCTGCAATG 
CGTGATGCTG TGAAAAAGTT AGGAGGAGAT CCAGAGAAAA TAAACCCTGT CTGCCCTGCT 
GATCTTGTAA TAGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAATT TGAAAGAAAT AGAGAGCGAT TTGAATTTTT AAAGTGGGGT 
TCCCAGGCTT TTCACAACAT GCGGATTATT CCCCCTGGCT CAGGAATCAT CCACCAGGTG 
AATTTGGAAT ATTTGGCAAG AGTGGTATTT GATCAGGATG GATATTATTA CCCAGACAGC 
CTCGTGGGCA CAGACTCGCA CACTACCATG ATTGATGGCT TGGGCATTCT TGGTTGGGGT 
GTCGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CAATCAGTAT GGTGCTTCCT 
CAGGTGATTG GCTACAGGCT GATGGGGAAG CCCCACCCTC TGGTAACATC CACTGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGCCAG GTTGGGGTAG TGGGCAAATT TGTCGAGTTC 
TTCGGGCCTG GAGTAGCCCA GTTGTCCATT GCTGACCGAG CTACGATTGC TAACATGTGT 
CCAGAGTACG GAGCAACTGC TGCCTTTTTC CCAGTTGATG AAGTTAGTAT CACGTACCTG 
GTGCAAACAG GTCGTGATGA AGAAAAATTA AAGTATATTA AAAAATATCT TCAGGCTGTA 
GGAATGTTTC GAGATTTCAA TGACCCTTCT CAAGACCCAG ACTTCACCCA GGTTGTGGAA 
TTAGATTTGA AAACAGTAGT GCCTTGCTGT AGTGGACCCA AAAGGCCTCA GGACAAAGTT 
GCTGTGTCCG ACATGAAAAA GGACTTTGAG AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGATTCCAAG TTGCTCCTGA ACATCATAAT GACCATAAGA CCTTTATCTA TGATAACACT 
GAATTCACCC TTGCTCATGG TTCTGTGGTC ATTGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCGT CTGTGATGTT AGGGGCAGGA TTGTTAGCAA AGAAAGCTGT GGATGCTGGC 
CTGAACGTGA TGCCTTACAT CAAAACTAGC CTGTCTCCTG GGAGTGGCGT GGTCACCTAC 
TACCTACAAG AAAGCGGAGT CATGCCTTAT CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT TGGCAACAGT GGGCCTTTAC CTGAACCTGT GGTAGAAGCC 
ATCACACAGG GAGACCTTGT AGCTGTTGGA GTACTATCTG GAAACAGGAA TTTTGAAGGT 
CGAGTTCACC CCAACACCCG GGCCAACTAT TTAGCCTCTC CCCCCTTAGT AATAGCATAT 
GCAATTGCTG GAACCATCAG AATCGACTTT GAGAAAGAGC CATTGGGAGT AAATGCAAAG 
GGACAGCAGG TATTTCTGAA AGATATCTGG CCGACTAGAG ACGAGATCCA GGCAGTGGAG 
CGTCAGTATG TCATCCCGGG GATGTTTAAG GAAGTCTATC AGAAAATAGA GACTGTGAAT 
GAAAGCTGGA ATGCCTTAGC AACCCCATCA GATAAGCTGT TTTTCTGGAA TTCCAAATCT 
ACGTATATCA AATCACCACC ATTCTTTGAA AACCTGACTT TGGATCTTCA GCCCCCTAAA 
TCTATAGTGG ATGCCTATGT GCTGCTAAAT TTGGGAGATT CGGTAACAAC TGACCACATC 
TCCCCAGCTG GAAATATTGC AAGAAACAGT CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTAACTCCAC GAGAATTCAA CTCCTATGGC TCCCGCCGAG GTAATGACGC CGTCATGGCA 
CGGGGAACAT TTGCCAACAT TCGCTTGTTA AACAGATTTT TGAACAAGCA GGCACCACAG 
ACTATCCATC TGCCTTCTGG GGAAATCCTT GATGTGTTTG ATGCTGCTGA GCGGTACCAG 
CAGGCAGGCC TTCCCCTGAT CGTTCTGGCT GGCAAAGAGT ACGGTGCAGG CAGCTCCCGA 
GACTGGGCAG CTAAGGGCCC TTTCCTGCTG GGAATCAAAG CCGTCCTGGC CGAGAGCTAC 
GAGCGCATTC ACCGCAGTAA CCTGGTTGGG ATGGGTGTGA TCCCACTTGA ATATCTCCCT 
GGTGAGAATG CAGATGCCCT GGGGCTCACA GGGCAAGAAC GATACACTAT CATTATTCCA 
GAAAACCTCA AACCACAAAT GAAAGTCCAG GTCAAGCTGG ATACTGGCAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC TGATGTGGAG CTCACTTATT TCCTCAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACO1 ( XM_005251476.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251533.1 Homo sapiens aconitase 1 (ACO1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu17506
Clone ID Related Accession (Same CDS sequence)	NM_001362840.2 , XM_011517888.1 , NM_001278352.1 , NM_002197.2 , NM_001362840.1 , NM_002197.3 , XM_005251476.1 , XM_011517888.2
Accession Version	XM_005251476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	cytoplasmic aconitate hydratase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAACC CATTCGCACA CCTTGCTGAG CCATTGGATC CTGTACAACC AGGAAAGAAA 
TTCTTCAATT TGAATAAATT GGAGGATTCA AGATATGGGC GCTTACCATT TTCGATCAGA 
GTTCTTCTGG AAGCAGCCAT TCGGAATTGT GATGAGTTTT TGGTGAAGAA ACAGGATATT 
GAAAATATTC TACATTGGAA TGTCACGCAG CACAAGAACA TAGAAGTGCC ATTTAAGCCT 
GCTCGTGTCA TCCTGCAGGA CTTTACGGGT GTGCCCGCTG TGGTTGACTT TGCTGCAATG 
CGTGATGCTG TGAAAAAGTT AGGAGGAGAT CCAGAGAAAA TAAACCCTGT CTGCCCTGCT 
GATCTTGTAA TAGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAATT TGAAAGAAAT AGAGAGCGAT TTGAATTTTT AAAGTGGGGT 
TCCCAGGCTT TTCACAACAT GCGGATTATT CCCCCTGGCT CAGGAATCAT CCACCAGGTG 
AATTTGGAAT ATTTGGCAAG AGTGGTATTT GATCAGGATG GATATTATTA CCCAGACAGC 
CTCGTGGGCA CAGACTCGCA CACTACCATG ATTGATGGCT TGGGCATTCT TGGTTGGGGT 
GTCGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CAATCAGTAT GGTGCTTCCT 
CAGGTGATTG GCTACAGGCT GATGGGGAAG CCCCACCCTC TGGTAACATC CACTGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGCCAG GTTGGGGTAG TGGGCAAATT TGTCGAGTTC 
TTCGGGCCTG GAGTAGCCCA GTTGTCCATT GCTGACCGAG CTACGATTGC TAACATGTGT 
CCAGAGTACG GAGCAACTGC TGCCTTTTTC CCAGTTGATG AAGTTAGTAT CACGTACCTG 
GTGCAAACAG GTCGTGATGA AGAAAAATTA AAGTATATTA AAAAATATCT TCAGGCTGTA 
GGAATGTTTC GAGATTTCAA TGACCCTTCT CAAGACCCAG ACTTCACCCA GGTTGTGGAA 
TTAGATTTGA AAACAGTAGT GCCTTGCTGT AGTGGACCCA AAAGGCCTCA GGACAAAGTT 
GCTGTGTCCG ACATGAAAAA GGACTTTGAG AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGATTCCAAG TTGCTCCTGA ACATCATAAT GACCATAAGA CCTTTATCTA TGATAACACT 
GAATTCACCC TTGCTCATGG TTCTGTGGTC ATTGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCGT CTGTGATGTT AGGGGCAGGA TTGTTAGCAA AGAAAGCTGT GGATGCTGGC 
CTGAACGTGA TGCCTTACAT CAAAACTAGC CTGTCTCCTG GGAGTGGCGT GGTCACCTAC 
TACCTACAAG AAAGCGGAGT CATGCCTTAT CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT TGGCAACAGT GGGCCTTTAC CTGAACCTGT GGTAGAAGCC 
ATCACACAGG GAGACCTTGT AGCTGTTGGA GTACTATCTG GAAACAGGAA TTTTGAAGGT 
CGAGTTCACC CCAACACCCG GGCCAACTAT TTAGCCTCTC CCCCCTTAGT AATAGCATAT 
GCAATTGCTG GAACCATCAG AATCGACTTT GAGAAAGAGC CATTGGGAGT AAATGCAAAG 
GGACAGCAGG TATTTCTGAA AGATATCTGG CCGACTAGAG ACGAGATCCA GGCAGTGGAG 
CGTCAGTATG TCATCCCGGG GATGTTTAAG GAAGTCTATC AGAAAATAGA GACTGTGAAT 
GAAAGCTGGA ATGCCTTAGC AACCCCATCA GATAAGCTGT TTTTCTGGAA TTCCAAATCT 
ACGTATATCA AATCACCACC ATTCTTTGAA AACCTGACTT TGGATCTTCA GCCCCCTAAA 
TCTATAGTGG ATGCCTATGT GCTGCTAAAT TTGGGAGATT CGGTAACAAC TGACCACATC 
TCCCCAGCTG GAAATATTGC AAGAAACAGT CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTAACTCCAC GAGAATTCAA CTCCTATGGC TCCCGCCGAG GTAATGACGC CGTCATGGCA 
CGGGGAACAT TTGCCAACAT TCGCTTGTTA AACAGATTTT TGAACAAGCA GGCACCACAG 
ACTATCCATC TGCCTTCTGG GGAAATCCTT GATGTGTTTG ATGCTGCTGA GCGGTACCAG 
CAGGCAGGCC TTCCCCTGAT CGTTCTGGCT GGCAAAGAGT ACGGTGCAGG CAGCTCCCGA 
GACTGGGCAG CTAAGGGCCC TTTCCTGCTG GGAATCAAAG CCGTCCTGGC CGAGAGCTAC 
GAGCGCATTC ACCGCAGTAA CCTGGTTGGG ATGGGTGTGA TCCCACTTGA ATATCTCCCT 
GGTGAGAATG CAGATGCCCT GGGGCTCACA GGGCAAGAAC GATACACTAT CATTATTCCA 
GAAAACCTCA AACCACAAAT GAAAGTCCAG GTCAAGCTGG ATACTGGCAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC TGATGTGGAG CTCACTTATT TCCTCAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251533.1
CDS	166..2835
Translation	MSNPFAHLAEPLDPVQPGKKFFNLNKLEDSRYGRLPFSIRVLLEAAIRNCDEFLVKKQDIENILHWNVTQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNRERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGILGWGVGGIEAEAVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSITYLVQTGRDEEKLKYIKKYLQAVGMFRDFNDPSQDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPEHHNDHKTFIYDNTEFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLNVMPYIKTSLSPGSGVVTYYLQESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMIRKMAK

Target ORF information:

RefSeq Version	XM_005251476.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aconitase 1 (ACO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251476.1

ORF Insert Sequence:

ATGAGCAACC CATTCGCACA CCTTGCTGAG CCATTGGATC CTGTACAACC AGGAAAGAAA 
TTCTTCAATT TGAATAAATT GGAGGATTCA AGATATGGGC GCTTACCATT TTCGATCAGA 
GTTCTTCTGG AAGCAGCCAT TCGGAATTGT GATGAGTTTT TGGTGAAGAA ACAGGATATT 
GAAAATATTC TACATTGGAA TGTCACGCAG CACAAGAACA TAGAAGTGCC ATTTAAGCCT 
GCTCGTGTCA TCCTGCAGGA CTTTACGGGT GTGCCCGCTG TGGTTGACTT TGCTGCAATG 
CGTGATGCTG TGAAAAAGTT AGGAGGAGAT CCAGAGAAAA TAAACCCTGT CTGCCCTGCT 
GATCTTGTAA TAGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAATT TGAAAGAAAT AGAGAGCGAT TTGAATTTTT AAAGTGGGGT 
TCCCAGGCTT TTCACAACAT GCGGATTATT CCCCCTGGCT CAGGAATCAT CCACCAGGTG 
AATTTGGAAT ATTTGGCAAG AGTGGTATTT GATCAGGATG GATATTATTA CCCAGACAGC 
CTCGTGGGCA CAGACTCGCA CACTACCATG ATTGATGGCT TGGGCATTCT TGGTTGGGGT 
GTCGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CAATCAGTAT GGTGCTTCCT 
CAGGTGATTG GCTACAGGCT GATGGGGAAG CCCCACCCTC TGGTAACATC CACTGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGCCAG GTTGGGGTAG TGGGCAAATT TGTCGAGTTC 
TTCGGGCCTG GAGTAGCCCA GTTGTCCATT GCTGACCGAG CTACGATTGC TAACATGTGT 
CCAGAGTACG GAGCAACTGC TGCCTTTTTC CCAGTTGATG AAGTTAGTAT CACGTACCTG 
GTGCAAACAG GTCGTGATGA AGAAAAATTA AAGTATATTA AAAAATATCT TCAGGCTGTA 
GGAATGTTTC GAGATTTCAA TGACCCTTCT CAAGACCCAG ACTTCACCCA GGTTGTGGAA 
TTAGATTTGA AAACAGTAGT GCCTTGCTGT AGTGGACCCA AAAGGCCTCA GGACAAAGTT 
GCTGTGTCCG ACATGAAAAA GGACTTTGAG AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGATTCCAAG TTGCTCCTGA ACATCATAAT GACCATAAGA CCTTTATCTA TGATAACACT 
GAATTCACCC TTGCTCATGG TTCTGTGGTC ATTGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCGT CTGTGATGTT AGGGGCAGGA TTGTTAGCAA AGAAAGCTGT GGATGCTGGC 
CTGAACGTGA TGCCTTACAT CAAAACTAGC CTGTCTCCTG GGAGTGGCGT GGTCACCTAC 
TACCTACAAG AAAGCGGAGT CATGCCTTAT CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT TGGCAACAGT GGGCCTTTAC CTGAACCTGT GGTAGAAGCC 
ATCACACAGG GAGACCTTGT AGCTGTTGGA GTACTATCTG GAAACAGGAA TTTTGAAGGT 
CGAGTTCACC CCAACACCCG GGCCAACTAT TTAGCCTCTC CCCCCTTAGT AATAGCATAT 
GCAATTGCTG GAACCATCAG AATCGACTTT GAGAAAGAGC CATTGGGAGT AAATGCAAAG 
GGACAGCAGG TATTTCTGAA AGATATCTGG CCGACTAGAG ACGAGATCCA GGCAGTGGAG 
CGTCAGTATG TCATCCCGGG GATGTTTAAG GAAGTCTATC AGAAAATAGA GACTGTGAAT 
GAAAGCTGGA ATGCCTTAGC AACCCCATCA GATAAGCTGT TTTTCTGGAA TTCCAAATCT 
ACGTATATCA AATCACCACC ATTCTTTGAA AACCTGACTT TGGATCTTCA GCCCCCTAAA 
TCTATAGTGG ATGCCTATGT GCTGCTAAAT TTGGGAGATT CGGTAACAAC TGACCACATC 
TCCCCAGCTG GAAATATTGC AAGAAACAGT CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTAACTCCAC GAGAATTCAA CTCCTATGGC TCCCGCCGAG GTAATGACGC CGTCATGGCA 
CGGGGAACAT TTGCCAACAT TCGCTTGTTA AACAGATTTT TGAACAAGCA GGCACCACAG 
ACTATCCATC TGCCTTCTGG GGAAATCCTT GATGTGTTTG ATGCTGCTGA GCGGTACCAG 
CAGGCAGGCC TTCCCCTGAT CGTTCTGGCT GGCAAAGAGT ACGGTGCAGG CAGCTCCCGA 
GACTGGGCAG CTAAGGGCCC TTTCCTGCTG GGAATCAAAG CCGTCCTGGC CGAGAGCTAC 
GAGCGCATTC ACCGCAGTAA CCTGGTTGGG ATGGGTGTGA TCCCACTTGA ATATCTCCCT 
GGTGAGAATG CAGATGCCCT GGGGCTCACA GGGCAAGAAC GATACACTAT CATTATTCCA 
GAAAACCTCA AACCACAAAT GAAAGTCCAG GTCAAGCTGG ATACTGGCAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC TGATGTGGAG CTCACTTATT TCCTCAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACO1 ( XM_011517888.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011516190.1 Homo sapiens aconitase 1 (ACO1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu17506
Clone ID Related Accession (Same CDS sequence)	NM_001362840.2 , XM_011517888.1 , NM_001278352.1 , NM_002197.2 , NM_001362840.1 , NM_002197.3 , XM_005251476.1 , XM_011517888.2
Accession Version	XM_011517888.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cytoplasmic aconitate hydratase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011517888.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAACC CATTCGCACA CCTTGCTGAG CCATTGGATC CTGTACAACC AGGAAAGAAA 
TTCTTCAATT TGAATAAATT GGAGGATTCA AGATATGGGC GCTTACCATT TTCGATCAGA 
GTTCTTCTGG AAGCAGCCAT TCGGAATTGT GATGAGTTTT TGGTGAAGAA ACAGGATATT 
GAAAATATTC TACATTGGAA TGTCACGCAG CACAAGAACA TAGAAGTGCC ATTTAAGCCT 
GCTCGTGTCA TCCTGCAGGA CTTTACGGGT GTGCCCGCTG TGGTTGACTT TGCTGCAATG 
CGTGATGCTG TGAAAAAGTT AGGAGGAGAT CCAGAGAAAA TAAACCCTGT CTGCCCTGCT 
GATCTTGTAA TAGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAATT TGAAAGAAAT AGAGAGCGAT TTGAATTTTT AAAGTGGGGT 
TCCCAGGCTT TTCACAACAT GCGGATTATT CCCCCTGGCT CAGGAATCAT CCACCAGGTG 
AATTTGGAAT ATTTGGCAAG AGTGGTATTT GATCAGGATG GATATTATTA CCCAGACAGC 
CTCGTGGGCA CAGACTCGCA CACTACCATG ATTGATGGCT TGGGCATTCT TGGTTGGGGT 
GTCGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CAATCAGTAT GGTGCTTCCT 
CAGGTGATTG GCTACAGGCT GATGGGGAAG CCCCACCCTC TGGTAACATC CACTGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGCCAG GTTGGGGTAG TGGGCAAATT TGTCGAGTTC 
TTCGGGCCTG GAGTAGCCCA GTTGTCCATT GCTGACCGAG CTACGATTGC TAACATGTGT 
CCAGAGTACG GAGCAACTGC TGCCTTTTTC CCAGTTGATG AAGTTAGTAT CACGTACCTG 
GTGCAAACAG GTCGTGATGA AGAAAAATTA AAGTATATTA AAAAATATCT TCAGGCTGTA 
GGAATGTTTC GAGATTTCAA TGACCCTTCT CAAGACCCAG ACTTCACCCA GGTTGTGGAA 
TTAGATTTGA AAACAGTAGT GCCTTGCTGT AGTGGACCCA AAAGGCCTCA GGACAAAGTT 
GCTGTGTCCG ACATGAAAAA GGACTTTGAG AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGATTCCAAG TTGCTCCTGA ACATCATAAT GACCATAAGA CCTTTATCTA TGATAACACT 
GAATTCACCC TTGCTCATGG TTCTGTGGTC ATTGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCGT CTGTGATGTT AGGGGCAGGA TTGTTAGCAA AGAAAGCTGT GGATGCTGGC 
CTGAACGTGA TGCCTTACAT CAAAACTAGC CTGTCTCCTG GGAGTGGCGT GGTCACCTAC 
TACCTACAAG AAAGCGGAGT CATGCCTTAT CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT TGGCAACAGT GGGCCTTTAC CTGAACCTGT GGTAGAAGCC 
ATCACACAGG GAGACCTTGT AGCTGTTGGA GTACTATCTG GAAACAGGAA TTTTGAAGGT 
CGAGTTCACC CCAACACCCG GGCCAACTAT TTAGCCTCTC CCCCCTTAGT AATAGCATAT 
GCAATTGCTG GAACCATCAG AATCGACTTT GAGAAAGAGC CATTGGGAGT AAATGCAAAG 
GGACAGCAGG TATTTCTGAA AGATATCTGG CCGACTAGAG ACGAGATCCA GGCAGTGGAG 
CGTCAGTATG TCATCCCGGG GATGTTTAAG GAAGTCTATC AGAAAATAGA GACTGTGAAT 
GAAAGCTGGA ATGCCTTAGC AACCCCATCA GATAAGCTGT TTTTCTGGAA TTCCAAATCT 
ACGTATATCA AATCACCACC ATTCTTTGAA AACCTGACTT TGGATCTTCA GCCCCCTAAA 
TCTATAGTGG ATGCCTATGT GCTGCTAAAT TTGGGAGATT CGGTAACAAC TGACCACATC 
TCCCCAGCTG GAAATATTGC AAGAAACAGT CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTAACTCCAC GAGAATTCAA CTCCTATGGC TCCCGCCGAG GTAATGACGC CGTCATGGCA 
CGGGGAACAT TTGCCAACAT TCGCTTGTTA AACAGATTTT TGAACAAGCA GGCACCACAG 
ACTATCCATC TGCCTTCTGG GGAAATCCTT GATGTGTTTG ATGCTGCTGA GCGGTACCAG 
CAGGCAGGCC TTCCCCTGAT CGTTCTGGCT GGCAAAGAGT ACGGTGCAGG CAGCTCCCGA 
GACTGGGCAG CTAAGGGCCC TTTCCTGCTG GGAATCAAAG CCGTCCTGGC CGAGAGCTAC 
GAGCGCATTC ACCGCAGTAA CCTGGTTGGG ATGGGTGTGA TCCCACTTGA ATATCTCCCT 
GGTGAGAATG CAGATGCCCT GGGGCTCACA GGGCAAGAAC GATACACTAT CATTATTCCA 
GAAAACCTCA AACCACAAAT GAAAGTCCAG GTCAAGCTGG ATACTGGCAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC TGATGTGGAG CTCACTTATT TCCTCAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011516190.1
CDS	73..2742
Translation	MSNPFAHLAEPLDPVQPGKKFFNLNKLEDSRYGRLPFSIRVLLEAAIRNCDEFLVKKQDIENILHWNVTQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNRERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGILGWGVGGIEAEAVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSITYLVQTGRDEEKLKYIKKYLQAVGMFRDFNDPSQDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPEHHNDHKTFIYDNTEFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLNVMPYIKTSLSPGSGVVTYYLQESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMIRKMAK

Target ORF information:

RefSeq Version	XM_011517888.2
Organism	Homo sapiens(human)
Definition	Homo sapiens aconitase 1 (ACO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011517888.2

ORF Insert Sequence:

ATGAGCAACC CATTCGCACA CCTTGCTGAG CCATTGGATC CTGTACAACC AGGAAAGAAA 
TTCTTCAATT TGAATAAATT GGAGGATTCA AGATATGGGC GCTTACCATT TTCGATCAGA 
GTTCTTCTGG AAGCAGCCAT TCGGAATTGT GATGAGTTTT TGGTGAAGAA ACAGGATATT 
GAAAATATTC TACATTGGAA TGTCACGCAG CACAAGAACA TAGAAGTGCC ATTTAAGCCT 
GCTCGTGTCA TCCTGCAGGA CTTTACGGGT GTGCCCGCTG TGGTTGACTT TGCTGCAATG 
CGTGATGCTG TGAAAAAGTT AGGAGGAGAT CCAGAGAAAA TAAACCCTGT CTGCCCTGCT 
GATCTTGTAA TAGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAATT TGAAAGAAAT AGAGAGCGAT TTGAATTTTT AAAGTGGGGT 
TCCCAGGCTT TTCACAACAT GCGGATTATT CCCCCTGGCT CAGGAATCAT CCACCAGGTG 
AATTTGGAAT ATTTGGCAAG AGTGGTATTT GATCAGGATG GATATTATTA CCCAGACAGC 
CTCGTGGGCA CAGACTCGCA CACTACCATG ATTGATGGCT TGGGCATTCT TGGTTGGGGT 
GTCGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CAATCAGTAT GGTGCTTCCT 
CAGGTGATTG GCTACAGGCT GATGGGGAAG CCCCACCCTC TGGTAACATC CACTGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGCCAG GTTGGGGTAG TGGGCAAATT TGTCGAGTTC 
TTCGGGCCTG GAGTAGCCCA GTTGTCCATT GCTGACCGAG CTACGATTGC TAACATGTGT 
CCAGAGTACG GAGCAACTGC TGCCTTTTTC CCAGTTGATG AAGTTAGTAT CACGTACCTG 
GTGCAAACAG GTCGTGATGA AGAAAAATTA AAGTATATTA AAAAATATCT TCAGGCTGTA 
GGAATGTTTC GAGATTTCAA TGACCCTTCT CAAGACCCAG ACTTCACCCA GGTTGTGGAA 
TTAGATTTGA AAACAGTAGT GCCTTGCTGT AGTGGACCCA AAAGGCCTCA GGACAAAGTT 
GCTGTGTCCG ACATGAAAAA GGACTTTGAG AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGATTCCAAG TTGCTCCTGA ACATCATAAT GACCATAAGA CCTTTATCTA TGATAACACT 
GAATTCACCC TTGCTCATGG TTCTGTGGTC ATTGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCGT CTGTGATGTT AGGGGCAGGA TTGTTAGCAA AGAAAGCTGT GGATGCTGGC 
CTGAACGTGA TGCCTTACAT CAAAACTAGC CTGTCTCCTG GGAGTGGCGT GGTCACCTAC 
TACCTACAAG AAAGCGGAGT CATGCCTTAT CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT TGGCAACAGT GGGCCTTTAC CTGAACCTGT GGTAGAAGCC 
ATCACACAGG GAGACCTTGT AGCTGTTGGA GTACTATCTG GAAACAGGAA TTTTGAAGGT 
CGAGTTCACC CCAACACCCG GGCCAACTAT TTAGCCTCTC CCCCCTTAGT AATAGCATAT 
GCAATTGCTG GAACCATCAG AATCGACTTT GAGAAAGAGC CATTGGGAGT AAATGCAAAG 
GGACAGCAGG TATTTCTGAA AGATATCTGG CCGACTAGAG ACGAGATCCA GGCAGTGGAG 
CGTCAGTATG TCATCCCGGG GATGTTTAAG GAAGTCTATC AGAAAATAGA GACTGTGAAT 
GAAAGCTGGA ATGCCTTAGC AACCCCATCA GATAAGCTGT TTTTCTGGAA TTCCAAATCT 
ACGTATATCA AATCACCACC ATTCTTTGAA AACCTGACTT TGGATCTTCA GCCCCCTAAA 
TCTATAGTGG ATGCCTATGT GCTGCTAAAT TTGGGAGATT CGGTAACAAC TGACCACATC 
TCCCCAGCTG GAAATATTGC AAGAAACAGT CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTAACTCCAC GAGAATTCAA CTCCTATGGC TCCCGCCGAG GTAATGACGC CGTCATGGCA 
CGGGGAACAT TTGCCAACAT TCGCTTGTTA AACAGATTTT TGAACAAGCA GGCACCACAG 
ACTATCCATC TGCCTTCTGG GGAAATCCTT GATGTGTTTG ATGCTGCTGA GCGGTACCAG 
CAGGCAGGCC TTCCCCTGAT CGTTCTGGCT GGCAAAGAGT ACGGTGCAGG CAGCTCCCGA 
GACTGGGCAG CTAAGGGCCC TTTCCTGCTG GGAATCAAAG CCGTCCTGGC CGAGAGCTAC 
GAGCGCATTC ACCGCAGTAA CCTGGTTGGG ATGGGTGTGA TCCCACTTGA ATATCTCCCT 
GGTGAGAATG CAGATGCCCT GGGGCTCACA GGGCAAGAAC GATACACTAT CATTATTCCA 
GAAAACCTCA AACCACAAAT GAAAGTCCAG GTCAAGCTGG ATACTGGCAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC TGATGTGGAG CTCACTTATT TCCTCAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GGCCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evaluation of the iron regulatory protein-1 interactome.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine31(1)139-146(2018 Feb)
Connell GJ,Danial JS,Haastruthers CX

A synergistic role of IRP1 and FBXL5 proteins in coordinating iron metabolism during cell proliferation.
The Journal of biological chemistry292(38)15976-15989(2017 09)
Johnson NB,Deck KM,Nizzi CP,Eisenstein RS

Pathological changes in Sertoli cells and dysregulation of divalent metal transporter 1 with iron responsive element in?the testes of idiopathic azoospermia patients.
Andrologia(2017 Aug)
Jing T,Wang P,Liu Y,Zhao J,Niu X,Wang X

Biogenesis and functions of mammalian iron-sulfur proteins in the regulation of iron homeostasis and pivotal metabolic pathways.
The Journal of biological chemistry292(31)12744-12753(2017 08)
Rouault TA,Maio N

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ACO1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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