Irp-1B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Irp-1B
Entrez Gene ID	41269
Full Name	Iron regulatory protein 1B
Synonyms	144037_at,C-Acon,CG6342,Dmel\CG6342,IRF,IRP,IRP-1,IRP-1B,IRP1,cACON,cAcon,dIRP,irp-1B,irp1B
General protein information	Preferred Names Iron regulatory protein 1B Names CG6342 gene product from transcript CG6342-RA CG6342-PA Irp-1B-PA c-aconitase cytoplasmic aconitase cytosolic aconitase iron regulatory factor iron regulatory protein iron regulatory protein 1B
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86B4-86B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_079579.3	NP_524303.2	iron regulatory protein 1B

Pathways from BioSystems

Homologies

Homo sapiens (human)	ACO1	NP_002188.1
Pan troglodytes (chimpanzee)	ACO1	XP_001156102.1
Drosophila melanogaster (fruit fly)	Irp-1A	NP_477371.1
Arabidopsis thaliana (thale cress)	ACO1	NP_195308.1
	ACO1	XP_001103761.1
Bos taurus (cattle)	ACO1	NP_001069059.1
Arabidopsis thaliana (thale cress)	ACO3	NP_178634.2
Caenorhabditis elegans (roundworm)	aco-1	NP_509898.1
Oryza sativa (rice)	Os03g0136900	NP_001048898.1
Canis lupus familiaris (dog)	ACO1	XP_538698.2
Rattus norvegicus (Norway rat)	Aco1	NP_059017.1
Danio rerio (zebrafish)	aco1	NP_001030155.1
Drosophila melanogaster (fruit fly)	Irp-1B	NP_524303.2
Xenopus tropicalis (tropical clawed frog)	aco1	NP_001135643.1
Mus musculus (house mouse)	Aco1	NP_031412.2
Gallus gallus (chicken)	ACO1	NP_001025707.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007258	XP_308544.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

muscle-specific overexpression of IRP-1A, but not of IRP-1B, resulted in pre-adult lethality, underscoring the importance of the biochemical difference between the two proteins;only IRP-1A can bind to iron-responsive elements
Title: Of two cytosolic aconitases expressed in Drosophila, only one functions as an iron-regulatory protein.

The following Irp-1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Irp-1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08294	NM_079579.3 Latest version!	Drosophila melanogaster iron regulatory protein 1B (Irp-1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Irp-1B ( NM_079579.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524303.2 Drosophila melanogaster iron regulatory protein 1B (Irp-1B), mRNA.

Price & Availability↑

CloneID	OFa08294
Clone ID Related Accession (Same CDS sequence)	NM_079579.3
Accession Version	NM_079579.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	iron regulatory protein 1B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079579.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAGGCG CCAATCCCTT CGCCCAGTTC GAGAAAACTT TCTCCCAGGC CGGCACAACA 
TACAAGTACT TTGACCTCGC CTCCATCGAT AGTAAATATG ATCAACTGCC CTACTCGATC 
CGAGTGCTCT TGGAGTCGGC GGTTCGCAAC TGCGACAACT TCCATATCCT AGAGAAGGAC 
GTGCAGAGCA TCCTGGGATG GTCGCCCGCC CTCAAGCAGG GATCCAACGA TGTGGAGGTG 
TCCTTCAAGC CGGCCCGTGT CATCCTGCAG GACTTTACTG GAGTTCCTGC TGTGGTGGAC 
TTTGCAGCTA TGCGCGATGC TGTTTTGGAT CTGGGCGGCG ACCCGGAGAA AATCAATCCC 
ATCTGCCCCG CCGACTTGGT CATCGATCAC TCGGTCCAGG TGGACTTCGC CCGTGCGCCT 
GATGCTTTGG CCAAGAACCA GTCACTGGAA TTTGAGCGCA ACAAGGAGCG CTTCACCTTC 
CTGAAGTGGG GAGCCAAGGC CTTCAACAAC ATGCTGATCG TGCCACCTGG CTCCGGTATT 
GTCCACCAGG TGAATCTGGA GTACTTGGCC CGCGTGGTGT TCGAGAATGA CGCAACCGAT 
GGCTCCAAGA TTCTGTATCC CGACAGTGTG GTGGGCACTG ATTCCCATAC CACGATGATA 
AATGGATTGG GCGTACTTGG TTGGGGAGTG GGAGGCATCG AAGCGGAAGC TGTGATGTTG 
GGCCAATCGA TTTCCATGTT GTTGCCCGAG GTAATTGGCT ACAAGTTGGA GGGCAAACTG 
AGCCCACTGG TCACATCCAC CGATCTGGTG CTGACCATTA CCAAGCACCT GCGCCAGTTG 
GGTGTGGTGG GAAAGTTTGT GGAGTTTTAC GGCCCGGGAG TTGCGGAACT CAGCATTGCT 
GACAGGGCCA CCATCAGCAA TATGTGTCCC GAATACGGAG CCACCGTGGG ATACTTCCCC 
ATCGATGAGA ATACTCTTGG TTACATGAAG CAGACAAATC GGTCTGAAAA GAAGATCGAC 
ATCATTCGCC AGTATCTGAA GGCAACTCAG CAGCTGCGCA ACTATGCTGA TGCGGCCCAA 
GATCCCAAGT TTACTCAGAG TATCACCTTG GATCTGTCGA CGGTGGTCAC CTCCGTTTCG 
GGACCCAAGC GTCCTCACGA TCGCGTTTCT GTCTCCGACA TGCCTGAGGA TTTTAAGTCG 
TGCCTGTCCA GCCCAGTGGG TTTCAAGGGA TTCGCCATTG CTCCGGAAGC TCAATCCGCT 
TTTGGGGAGT TCCAGTGGGA TGATGGCAAG ACCTACAAAC TGCATCATGG ATCTGTTGTC 
ATTGCGGCCA TCACATCCTG CACGAACACA TCGAATCCTT CGGTGATGCT GGGTGCCGGT 
CTGCTGGCCA AAAAGGCCGT TGAGAAGGGT CTGAGCATCC TGCCCTATAT CAAGACCTCG 
CTGTCGCCTG GATCCGGTGT GGTTACCTAC TACCTGAAAG AGTCCGGAGT CATTCCCTAT 
CTGGAGAAGT TGGGCTTCGA TATTGTGGGC TATGGATGCA TGACCTGCAT TGGTAACTCC 
GGTCCTCTCG AGGAGAATGT GGTGAACACC ATCGAGAAGA ACGGCCTCGT GTGTGCTGGA 
GTTTTGTCCG GAAACCGAAA CTTCGAGGGT CGCATCCATC CCAACACCAG GGCCAACTAT 
CTGGCCAGTC CTCTGTTGGT GATCGCATAT GCCATCGCAG GACGCGTGGA CATCGACTTC 
GAGAAGGAAC CTCTGGGAGT GGATGCCAAT GGCAAGAATG TGTTCTTGCA GGACATCTGG 
CCAACGCGAT CGGAGATACA GGAAGTGGAG AACAAGCATG TAATCCCCGC CATGTTCCAG 
GAGGTGTACA GCAAAATTGA GCTGGGCTCC CAGGATTGGC AGACTCTGCA GGTGTCCGAG 
GGCAAGCTCT TCTCCTGGAG TGCCGATTCC ACCTACATTA AGCGACCACC CTTCTTCGAG 
GGCATGACCA GAGATCTTCC CAAGCTGCAG AGCATTCAGA AGGCACGTTG CCTGCTCTTC 
CTAGGCGATT CCGTCACCAC GGATCACATC TCGCCGGCTG GATCTATTGC TAGAACCTCG 
CCTGCTGCCC GATTCTTGTC CGAGCGGAAC ATCACACCCC GTGACTTCAA CTCGTACGGA 
TCGCGTCGTG GCAACGATGC CATCATGTCG CGCGGCACCT TCGCCAACAT TCGGTTGGTC 
AACAAACTGG TCGAAAAGAC AGGACCCCGC ACCGTGCACA TTCCCAGCCA GGAAGAGCTG 
GATATCTTCG ATGCCGCCGA GCGGTACCGC GAGGAGGGTA CTCCTCTGGT CCTCGTTGTA 
GGCAAGGACT ACGGCAGCGG AAGCTCTCGG GATTGGGCCG CCAAGGGTCC CTTCCTGCTG 
GGCGTCAAGG CGGTGATCGC GGAGTCCTAC GAGCGCATTC ATCGTTCCAA CCTGGTGGGC 
ATGGGCATCA TTCCGCTGCA ATTCCTGCCG GGACAGAGTG CCGAGACATT GAACCTGACT 
GGCCGGGAGG TGTACAACAT CGCCCTGCCA GAGAGCGGTT TAAAGCCGGG CCAGAAGATC 
CAAGTGGAGG CTGATGGCAC CGTCTTTGAG ACTATTCTGC GCTTCGACAC TGAGGTGGAC 
ATCACCTACT ACAAGAATGG TGGAATCCTC AACTACATGA TTCGCAAAAT GCTCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524303.2
CDS	55..2754
Translation	MSGANPFAQFEKTFSQAGTTYKYFDLASIDSKYDQLPYSIRVLLESAVRNCDNFHILEKDVQSILGWSPALKQGSNDVEVSFKPARVILQDFTGVPAVVDFAAMRDAVLDLGGDPEKINPICPADLVIDHSVQVDFARAPDALAKNQSLEFERNKERFTFLKWGAKAFNNMLIVPPGSGIVHQVNLEYLARVVFENDATDGSKILYPDSVVGTDSHTTMINGLGVLGWGVGGIEAEAVMLGQSISMLLPEVIGYKLEGKLSPLVTSTDLVLTITKHLRQLGVVGKFVEFYGPGVAELSIADRATISNMCPEYGATVGYFPIDENTLGYMKQTNRSEKKIDIIRQYLKATQQLRNYADAAQDPKFTQSITLDLSTVVTSVSGPKRPHDRVSVSDMPEDFKSCLSSPVGFKGFAIAPEAQSAFGEFQWDDGKTYKLHHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVEKGLSILPYIKTSLSPGSGVVTYYLKESGVIPYLEKLGFDIVGYGCMTCIGNSGPLEENVVNTIEKNGLVCAGVLSGNRNFEGRIHPNTRANYLASPLLVIAYAIAGRVDIDFEKEPLGVDANGKNVFLQDIWPTRSEIQEVENKHVIPAMFQEVYSKIELGSQDWQTLQVSEGKLFSWSADSTYIKRPPFFEGMTRDLPKLQSIQKARCLLFLGDSVTTDHISPAGSIARTSPAARFLSERNITPRDFNSYGSRRGNDAIMSRGTFANIRLVNKLVEKTGPRTVHIPSQEELDIFDAAERYREEGTPLVLVVGKDYGSGSSRDWAAKGPFLLGVKAVIAESYERIHRSNLVGMGIIPLQFLPGQSAETLNLTGREVYNIALPESGLKPGQKIQVEADGTVFETILRFDTEVDITYYKNGGILNYMIRKMLS

Target ORF information:

RefSeq Version	NM_079579.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster iron regulatory protein 1B (Irp-1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079579.3

ORF Insert Sequence:

ATGTCAGGCG CCAATCCCTT CGCCCAGTTC GAGAAAACTT TCTCCCAGGC CGGCACAACA 
TACAAGTACT TTGACCTCGC CTCCATCGAT AGTAAATATG ATCAACTGCC CTACTCGATC 
CGAGTGCTCT TGGAGTCGGC GGTTCGCAAC TGCGACAACT TCCATATCCT AGAGAAGGAC 
GTGCAGAGCA TCCTGGGATG GTCGCCCGCC CTCAAGCAGG GATCCAACGA TGTGGAGGTG 
TCCTTCAAGC CGGCCCGTGT CATCCTGCAG GACTTTACTG GAGTTCCTGC TGTGGTGGAC 
TTTGCAGCTA TGCGCGATGC TGTTTTGGAT CTGGGCGGCG ACCCGGAGAA AATCAATCCC 
ATCTGCCCCG CCGACTTGGT CATCGATCAC TCGGTCCAGG TGGACTTCGC CCGTGCGCCT 
GATGCTTTGG CCAAGAACCA GTCACTGGAA TTTGAGCGCA ACAAGGAGCG CTTCACCTTC 
CTGAAGTGGG GAGCCAAGGC CTTCAACAAC ATGCTGATCG TGCCACCTGG CTCCGGTATT 
GTCCACCAGG TGAATCTGGA GTACTTGGCC CGCGTGGTGT TCGAGAATGA CGCAACCGAT 
GGCTCCAAGA TTCTGTATCC CGACAGTGTG GTGGGCACTG ATTCCCATAC CACGATGATA 
AATGGATTGG GCGTACTTGG TTGGGGAGTG GGAGGCATCG AAGCGGAAGC TGTGATGTTG 
GGCCAATCGA TTTCCATGTT GTTGCCCGAG GTAATTGGCT ACAAGTTGGA GGGCAAACTG 
AGCCCACTGG TCACATCCAC CGATCTGGTG CTGACCATTA CCAAGCACCT GCGCCAGTTG 
GGTGTGGTGG GAAAGTTTGT GGAGTTTTAC GGCCCGGGAG TTGCGGAACT CAGCATTGCT 
GACAGGGCCA CCATCAGCAA TATGTGTCCC GAATACGGAG CCACCGTGGG ATACTTCCCC 
ATCGATGAGA ATACTCTTGG TTACATGAAG CAGACAAATC GGTCTGAAAA GAAGATCGAC 
ATCATTCGCC AGTATCTGAA GGCAACTCAG CAGCTGCGCA ACTATGCTGA TGCGGCCCAA 
GATCCCAAGT TTACTCAGAG TATCACCTTG GATCTGTCGA CGGTGGTCAC CTCCGTTTCG 
GGACCCAAGC GTCCTCACGA TCGCGTTTCT GTCTCCGACA TGCCTGAGGA TTTTAAGTCG 
TGCCTGTCCA GCCCAGTGGG TTTCAAGGGA TTCGCCATTG CTCCGGAAGC TCAATCCGCT 
TTTGGGGAGT TCCAGTGGGA TGATGGCAAG ACCTACAAAC TGCATCATGG ATCTGTTGTC 
ATTGCGGCCA TCACATCCTG CACGAACACA TCGAATCCTT CGGTGATGCT GGGTGCCGGT 
CTGCTGGCCA AAAAGGCCGT TGAGAAGGGT CTGAGCATCC TGCCCTATAT CAAGACCTCG 
CTGTCGCCTG GATCCGGTGT GGTTACCTAC TACCTGAAAG AGTCCGGAGT CATTCCCTAT 
CTGGAGAAGT TGGGCTTCGA TATTGTGGGC TATGGATGCA TGACCTGCAT TGGTAACTCC 
GGTCCTCTCG AGGAGAATGT GGTGAACACC ATCGAGAAGA ACGGCCTCGT GTGTGCTGGA 
GTTTTGTCCG GAAACCGAAA CTTCGAGGGT CGCATCCATC CCAACACCAG GGCCAACTAT 
CTGGCCAGTC CTCTGTTGGT GATCGCATAT GCCATCGCAG GACGCGTGGA CATCGACTTC 
GAGAAGGAAC CTCTGGGAGT GGATGCCAAT GGCAAGAATG TGTTCTTGCA GGACATCTGG 
CCAACGCGAT CGGAGATACA GGAAGTGGAG AACAAGCATG TAATCCCCGC CATGTTCCAG 
GAGGTGTACA GCAAAATTGA GCTGGGCTCC CAGGATTGGC AGACTCTGCA GGTGTCCGAG 
GGCAAGCTCT TCTCCTGGAG TGCCGATTCC ACCTACATTA AGCGACCACC CTTCTTCGAG 
GGCATGACCA GAGATCTTCC CAAGCTGCAG AGCATTCAGA AGGCACGTTG CCTGCTCTTC 
CTAGGCGATT CCGTCACCAC GGATCACATC TCGCCGGCTG GATCTATTGC TAGAACCTCG 
CCTGCTGCCC GATTCTTGTC CGAGCGGAAC ATCACACCCC GTGACTTCAA CTCGTACGGA 
TCGCGTCGTG GCAACGATGC CATCATGTCG CGCGGCACCT TCGCCAACAT TCGGTTGGTC 
AACAAACTGG TCGAAAAGAC AGGACCCCGC ACCGTGCACA TTCCCAGCCA GGAAGAGCTG 
GATATCTTCG ATGCCGCCGA GCGGTACCGC GAGGAGGGTA CTCCTCTGGT CCTCGTTGTA 
GGCAAGGACT ACGGCAGCGG AAGCTCTCGG GATTGGGCCG CCAAGGGTCC CTTCCTGCTG 
GGCGTCAAGG CGGTGATCGC GGAGTCCTAC GAGCGCATTC ATCGTTCCAA CCTGGTGGGC 
ATGGGCATCA TTCCGCTGCA ATTCCTGCCG GGACAGAGTG CCGAGACATT GAACCTGACT 
GGCCGGGAGG TGTACAACAT CGCCCTGCCA GAGAGCGGTT TAAAGCCGGG CCAGAAGATC 
CAAGTGGAGG CTGATGGCAC CGTCTTTGAG ACTATTCTGC GCTTCGACAC TGAGGTGGAC 
ATCACCTACT ACAAGAATGG TGGAATCCTC AACTACATGA TTCGCAAAAT GCTCTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Irp-1B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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