Aco1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aco1
Entrez Gene ID	11428
Full Name	aconitase 1
Synonyms	Aco-1,Irebp,Irp1
General protein information	Preferred Names aconitase 1 Names cytoplasmic aconitate hydratase IRE-BP 1 citrate hydro-lyase cytoplasmic aconitase iron regulatory protein 1 iron-responsive element-binding protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 A5\|4 20.24 cM
Summary	This gene encodes a member of the aconitase/IPM isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Depending on iron levels in the cytosol, the encoded protein can function as either an aconitase enzyme or as an mRNA binding protein. When cellular iron levels are high, the encoded protein functions as an aconitase, an essential enzyme in the TCA cycle that catalyzes the conversion of citrate to isocitrate. When cellular iron levels are low, the encoded protein regulates iron uptake and utilization by binding to iron-responsive elements in the untranslated regions of mRNAs for genes involved in iron metabolism. Disruption of this gene is associated with pulmonary hypertension and polycythemia. [provided by RefSeq, Jan 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_007386.2	NP_031412.2	cytoplasmic aconitate hydratase

Pathways from BioSystems

Homologies

Homo sapiens (human)	ACO1	NP_002188.1
Pan troglodytes (chimpanzee)	ACO1	XP_001156102.1
Drosophila melanogaster (fruit fly)	Irp-1A	NP_477371.1
Arabidopsis thaliana (thale cress)	ACO1	NP_195308.1
	ACO1	XP_001103761.1
Bos taurus (cattle)	ACO1	NP_001069059.1
Arabidopsis thaliana (thale cress)	ACO3	NP_178634.2
Caenorhabditis elegans (roundworm)	aco-1	NP_509898.1
Oryza sativa (rice)	Os03g0136900	NP_001048898.1
Canis lupus familiaris (dog)	ACO1	XP_538698.2
Rattus norvegicus (Norway rat)	Aco1	NP_059017.1
Danio rerio (zebrafish)	aco1	NP_001030155.1
Drosophila melanogaster (fruit fly)	Irp-1B	NP_524303.2
Xenopus tropicalis (tropical clawed frog)	aco1	NP_001135643.1
Mus musculus (house mouse)	Aco1	NP_031412.2
Gallus gallus (chicken)	ACO1	NP_001025707.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007258	XP_308544.4

Gene Ontology
Bibliography

Related articles in PubMed

FBXL5 Inactivation in Mouse Brain Induces Aberrant Proliferation of Neural Stem Progenitor Cells.
Yamauchi T, Nishiyama M, Moroishi T, Kawamura A, Nakayama KI
Molecular and cellular biology37(8)(2017 Apr)

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Interleukin-6 regulates iron-related proteins through c-Jun N-terminal kinase activation in BV2 microglial cell lines.
Zhou S, Du X, Xie J, Wang J
PloS one12(7)e0180464(2017)

A drastic superoxide-dependent oxidative stress is prerequisite for the down-regulation of IRP1: Insights from studies on SOD1-deficient mice and macrophages treated with paraquat.
Milczarek A, Starzy?ski RR, Sty? A, Jo?czy A, Staro? R, Grzelak A, Lipi?ski P
PloS one12(5)e0176800(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IL-6 likely up-regulates IRP1 and DMT1 expression and down-regulates FPN1 expression in BV2 microglial cells through JNK signaling pathways
Title: Interleukin-6 regulates iron-related proteins through c-Jun N-terminal kinase activation in BV2 microglial cell lines.

Using whole RNA sequencing of bone marrow cells in iron-overloaded mice, it was observed that Idh1 and Aco1, enzymes involved in the TCA cycle, were elevated.
Title: Iron-induced epigenetic abnormalities of mouse bone marrow through aberrant activation of aconitase and isocitrate dehydrogenase.

The Irp1-/- mice develop polycythemia and pulmonary hypertension, and suggesting that Irp1 plays an essential role in erythropoiesis and in the pulmonary and cardiovascular systems.
Title: Iron misregulation and neurodegenerative disease in mouse models that lack iron regulatory proteins.

IRP1 and IRP2 mutant mice rapidly succumb to systemic infection with Salmonella Typhimurium, a pathogenic bacterium that multiplies within macrophages, with increased bacterial burdens in liver and spleen.
Title: Iron Regulatory Proteins Mediate Host Resistance to Salmonella Infection.

results uncover an unexpected protective role of IRP1 in pathological conditions associated with altered Fe-S metabolism
Title: Iron regulatory protein 1 sustains mitochondrial iron loading and function in frataxin deficiency.

The following Aco1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aco1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22032	NM_007386.2 Latest version!	Mus musculus aconitase 1 (Aco1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aco1 ( NM_007386.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031412.2 Mus musculus aconitase 1 (Aco1), mRNA.

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CloneID	OMu22032
Clone ID Related Accession (Same CDS sequence)	NM_007386.2
Accession Version	NM_007386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	cytoplasmic aconitate hydratase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB854442.1, BY145469.1, AK163985.1 and AL831793.4. On Jul 14, 2006 this sequence version replaced NM_007386.1. Sequence Note: This sequence has been modified as follows: removed 28 bp suspected to be vector contamination from the 3' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.207336.1, AK163985.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## multifunctional gene product(s) :: PMID: 1946430 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGAACC CATTTGCACA CCTTGCTGAG CCCTTGGACG CTGCCCAACC AGGAAAGAGG 
TTCTTCAACT TGAATAAATT GGAGGACTCA AGATACGGAC GCTTACCATT TTCTATCAGA 
GTTCTCCTGG AGGCAGCCGT TCGGAACTGT GATGAGTTTC TGGTGAAGAA AAATGACATC 
GAGAATATCC TGAATTGGAA TGTCATGCAA CATAAGAACA TAGAAGTGCC GTTTAAGCCA 
GCCCGAGTCA TACTGCAAGA CTTTACGGGT GTGCCTGCTG TGGTGGATTT TGCAGCAATG 
CGCGATGCTG TGAAGAAGTT GGGAGGGAAT CCGGAGAAAA TTAACCCTGT CTGCCCCGCT 
GACCTTGTAA TTGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAGTT TGAAAGAAAT AAAGAACGAT TTGAATTTCT AAAGTGGGGT 
TCCCAGGCCT TTTGCAACAT GCGGATAATC CCTCCTGGCT CGGGAATCAT TCACCAAGTG 
AACCTGGAGT ATTTGGCAAG AGTAGTGTTT GATCAGGATG GATGCTACTA CCCAGACAGC 
CTCGTGGGCA CAGATTCACA CACGACCATG ATTGACGGCC TGGGAGTTCT TGGTTGGGGT 
GTGGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CCATCAGCAT GGTGCTTCCC 
CAGGTGATTG GCTACAAGCT GATGGGGAAG CCTCACCCTC TGGTGACATC CACGGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGACAA GTGGGAGTTG TGGGCAAATT TGTGGAGTTT 
TTCGGGCCAG GAGTGGCTCA GCTGTCCATT GCTGACCGAG CTACGATTGC CAATATGTGC 
CCAGAGTATG GTGCGACAGC AGCCTTCTTC CCGGTTGATG AAGTTAGCAT CGCGTACCTG 
CTGCAGACAG GCCGCGAGGA AGACAAAGTC AAGCACATTC AGAAGTATCT TCAGGCTGTA 
GGCATGTTTC GAGATTTCAA CGACACCTCT CAAGACCCAG ACTTCACTCA GGTTGTGGAG 
TTAGATCTGA AAACAGTTGT GCCTTGCTGC AGTGGACCCA AAAGACCTCA GGACAAAGTC 
GCGGTGTCTG AGATGAAAAA GGACTTTGAA AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGTTTCCAAG TTGCTCCAGA CCGTCACAAT GACCGCAAGA CGTTCCTCTA TAGTAACAGT 
GAATTCACTC TCGCTCATGG CTCTGTGGTA ATCGCTGCCA TCACTAGCTG CACAAACACC 
AGCAATCCAT CCGTGATGTT AGGAGCAGGA TTGTTAGCAA AGAAAGCTGT GGAGGCGGGG 
CTGAGTGTGA AGCCTTACAT CAAAACCAGC CTGTCTCCTG GAAGTGGAGT GGTCACCTAC 
TACCTTCGAG AGAGTGGAGT CATGCCTTAC CTGTCCCAGT TAGGGTTTGA TGTGGTGGGC 
TACGGCTGCA TGACCTGCAT CGGCAACAGT GGACCCCTTC CTGAACCTGT GGTCGAGGCA 
ATCACCCAGG GAGACCTCGT AGCTGTTGGC GTTCTGTCTG GGAATAGGAA TTTTGAAGGC 
CGAGTCCATC CTAACACACG AGCCAACTAC TTAGCATCCC CCCCTCTGGT AATAGCATAT 
GCGATTGCAG GCACCGTCAG GATTGACTTC GAGAAAGAGC CTTTGGGAGT GAACGCACAG 
GGCCGGCAAG TGTTTCTGAA GGACATCTGG CCCACGCGAG ATGAGATCCA GGCGGTGGAA 
AGGCAGCATG TCATCCCCGG GATGTTTAAG GAGGTCTATC AGAAAATAGA GACTGTAAAC 
AAAAGCTGGA ATGCCTTAGC AGCCCCGTCA GAGAAGCTGT ATGCGTGGAA TCCCAAATCT 
ACTTACATTA AGTCGCCACC ATTCTTTGAA AGCTTGACTT TAGATCTCCA GCCACCCAAG 
TCCATAGTGG ATGCCTATGT GCTACTGAAT TTAGGAGATT CAGTAACAAC GGACCATATC 
TCTCCCGCCG GCAATATTGC AAGAAACAGC CCTGCGGCTC GCTACTTGAC GAACAGAGGC 
CTAACACCAC GAGAGTTCAA CTCCTATGGC TCCCGCCGGG GTAACGACGC CATCATGGCC 
CGGGGGACAT TTGCCAACAT TCGCTTGCTG AACAAGTTTC TGAACAAGCA GGCACCTCAG 
ACTGTCCATC TCCCCTCAGG AGAAACCCTT GATGTATTCG ACGCTGCTGA GCGGTACCAG 
CAGGCTGGAC TTCCCCTGAT TGTTCTGGCT GGGAAAGAAT ACGGTTCAGG CAGCTCCCGA 
GACTGGGCAG CCAAAGGCCC TTTCCTGCTG GGAATCAAAG CTGTCCTGGC AGAGAGCTAT 
GAGCGCATTC ACCGCAGCAA CTTGGTTGGC ATGGGGGTGA TCCCCCTTGA GTATCTCCCT 
GGTGAAACTG CAGACTCTCT GGGACTCACA GGTCGGGAAA GATACACTAT CAACATCCCT 
GAAGACCTCA AGCCTCGCAT GACGGTGCAG ATCAAGCTGG ACACTGGGAA GACCTTCCAG 
GCCGTGATGA GGTTCGACAC TGATGTGGAG CTCACGTACT TCCACAATGG AGGCATCCTG 
AACTACATGA TCCGAAAGAT GGCCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031412.2
CDS	308..2977
Translation	MKNPFAHLAEPLDAAQPGKRFFNLNKLEDSRYGRLPFSIRVLLEAAVRNCDEFLVKKNDIENILNWNVMQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGNPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNKERFEFLKWGSQAFCNMRIIPPGSGIIHQVNLEYLARVVFDQDGCYYPDSLVGTDSHTTMIDGLGVLGWGVGGIEAEAVMLGQPISMVLPQVIGYKLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSIAYLLQTGREEDKVKHIQKYLQAVGMFRDFNDTSQDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSEMKKDFESCLGAKQGFKGFQVAPDRHNDRKTFLYSNSEFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVEAGLSVKPYIKTSLSPGSGVVTYYLRESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTVRIDFEKEPLGVNAQGRQVFLKDIWPTRDEIQAVERQHVIPGMFKEVYQKIETVNKSWNALAAPSEKLYAWNPKSTYIKSPPFFESLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAIMARGTFANIRLLNKFLNKQAPQTVHLPSGETLDVFDAAERYQQAGLPLIVLAGKEYGSGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGETADSLGLTGRERYTINIPEDLKPRMTVQIKLDTGKTFQAVMRFDTDVELTYFHNGGILNYMIRKMAQ

Target ORF information:

RefSeq Version	NM_007386.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus aconitase 1 (Aco1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007386.2

ORF Insert Sequence:

ATGAAGAACC CATTTGCACA CCTTGCTGAG CCCTTGGACG CTGCCCAACC AGGAAAGAGG 
TTCTTCAACT TGAATAAATT GGAGGACTCA AGATACGGAC GCTTACCATT TTCTATCAGA 
GTTCTCCTGG AGGCAGCCGT TCGGAACTGT GATGAGTTTC TGGTGAAGAA AAATGACATC 
GAGAATATCC TGAATTGGAA TGTCATGCAA CATAAGAACA TAGAAGTGCC GTTTAAGCCA 
GCCCGAGTCA TACTGCAAGA CTTTACGGGT GTGCCTGCTG TGGTGGATTT TGCAGCAATG 
CGCGATGCTG TGAAGAAGTT GGGAGGGAAT CCGGAGAAAA TTAACCCTGT CTGCCCCGCT 
GACCTTGTAA TTGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTACAG 
AAGAATCAAG ACCTGGAGTT TGAAAGAAAT AAAGAACGAT TTGAATTTCT AAAGTGGGGT 
TCCCAGGCCT TTTGCAACAT GCGGATAATC CCTCCTGGCT CGGGAATCAT TCACCAAGTG 
AACCTGGAGT ATTTGGCAAG AGTAGTGTTT GATCAGGATG GATGCTACTA CCCAGACAGC 
CTCGTGGGCA CAGATTCACA CACGACCATG ATTGACGGCC TGGGAGTTCT TGGTTGGGGT 
GTGGGTGGTA TTGAAGCAGA AGCTGTCATG CTGGGTCAGC CCATCAGCAT GGTGCTTCCC 
CAGGTGATTG GCTACAAGCT GATGGGGAAG CCTCACCCTC TGGTGACATC CACGGACATC 
GTGCTCACCA TTACCAAGCA CCTCCGACAA GTGGGAGTTG TGGGCAAATT TGTGGAGTTT 
TTCGGGCCAG GAGTGGCTCA GCTGTCCATT GCTGACCGAG CTACGATTGC CAATATGTGC 
CCAGAGTATG GTGCGACAGC AGCCTTCTTC CCGGTTGATG AAGTTAGCAT CGCGTACCTG 
CTGCAGACAG GCCGCGAGGA AGACAAAGTC AAGCACATTC AGAAGTATCT TCAGGCTGTA 
GGCATGTTTC GAGATTTCAA CGACACCTCT CAAGACCCAG ACTTCACTCA GGTTGTGGAG 
TTAGATCTGA AAACAGTTGT GCCTTGCTGC AGTGGACCCA AAAGACCTCA GGACAAAGTC 
GCGGTGTCTG AGATGAAAAA GGACTTTGAA AGCTGCCTTG GAGCCAAGCA AGGATTTAAA 
GGTTTCCAAG TTGCTCCAGA CCGTCACAAT GACCGCAAGA CGTTCCTCTA TAGTAACAGT 
GAATTCACTC TCGCTCATGG CTCTGTGGTA ATCGCTGCCA TCACTAGCTG CACAAACACC 
AGCAATCCAT CCGTGATGTT AGGAGCAGGA TTGTTAGCAA AGAAAGCTGT GGAGGCGGGG 
CTGAGTGTGA AGCCTTACAT CAAAACCAGC CTGTCTCCTG GAAGTGGAGT GGTCACCTAC 
TACCTTCGAG AGAGTGGAGT CATGCCTTAC CTGTCCCAGT TAGGGTTTGA TGTGGTGGGC 
TACGGCTGCA TGACCTGCAT CGGCAACAGT GGACCCCTTC CTGAACCTGT GGTCGAGGCA 
ATCACCCAGG GAGACCTCGT AGCTGTTGGC GTTCTGTCTG GGAATAGGAA TTTTGAAGGC 
CGAGTCCATC CTAACACACG AGCCAACTAC TTAGCATCCC CCCCTCTGGT AATAGCATAT 
GCGATTGCAG GCACCGTCAG GATTGACTTC GAGAAAGAGC CTTTGGGAGT GAACGCACAG 
GGCCGGCAAG TGTTTCTGAA GGACATCTGG CCCACGCGAG ATGAGATCCA GGCGGTGGAA 
AGGCAGCATG TCATCCCCGG GATGTTTAAG GAGGTCTATC AGAAAATAGA GACTGTAAAC 
AAAAGCTGGA ATGCCTTAGC AGCCCCGTCA GAGAAGCTGT ATGCGTGGAA TCCCAAATCT 
ACTTACATTA AGTCGCCACC ATTCTTTGAA AGCTTGACTT TAGATCTCCA GCCACCCAAG 
TCCATAGTGG ATGCCTATGT GCTACTGAAT TTAGGAGATT CAGTAACAAC GGACCATATC 
TCTCCCGCCG GCAATATTGC AAGAAACAGC CCTGCGGCTC GCTACTTGAC GAACAGAGGC 
CTAACACCAC GAGAGTTCAA CTCCTATGGC TCCCGCCGGG GTAACGACGC CATCATGGCC 
CGGGGGACAT TTGCCAACAT TCGCTTGCTG AACAAGTTTC TGAACAAGCA GGCACCTCAG 
ACTGTCCATC TCCCCTCAGG AGAAACCCTT GATGTATTCG ACGCTGCTGA GCGGTACCAG 
CAGGCTGGAC TTCCCCTGAT TGTTCTGGCT GGGAAAGAAT ACGGTTCAGG CAGCTCCCGA 
GACTGGGCAG CCAAAGGCCC TTTCCTGCTG GGAATCAAAG CTGTCCTGGC AGAGAGCTAT 
GAGCGCATTC ACCGCAGCAA CTTGGTTGGC ATGGGGGTGA TCCCCCTTGA GTATCTCCCT 
GGTGAAACTG CAGACTCTCT GGGACTCACA GGTCGGGAAA GATACACTAT CAACATCCCT 
GAAGACCTCA AGCCTCGCAT GACGGTGCAG ATCAAGCTGG ACACTGGGAA GACCTTCCAG 
GCCGTGATGA GGTTCGACAC TGATGTGGAG CTCACGTACT TCCACAATGG AGGCATCCTG 
AACTACATGA TCCGAAAGAT GGCCCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FBXL5 Inactivation in Mouse Brain Induces Aberrant Proliferation of Neural Stem Progenitor Cells.
Molecular and cellular biology37(8)(2017 Apr)
Yamauchi T,Nishiyama M,Moroishi T,Kawamura A,Nakayama KI

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Developmental cell40(4)331-341.e4(2017 02)
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

Interleukin-6 regulates iron-related proteins through c-Jun N-terminal kinase activation in BV2 microglial cell lines.
PloS one12(7)e0180464(2017)
Zhou S,Du X,Xie J,Wang J

A drastic superoxide-dependent oxidative stress is prerequisite for the down-regulation of IRP1: Insights from studies on SOD1-deficient mice and macrophages treated with paraquat.
PloS one12(5)e0176800(2017)
Milczarek A,Starzy?ski RR,Sty? A,Jo?czy A,Staro? R,Grzelak A,Lipi?ski P

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Aco1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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