ACO1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACO1
Entrez Gene ID	512995
Full Name	aconitase 1
General protein information	Preferred Names aconitase 1 Names cytoplasmic aconitate hydratase IRE-BP 1 aconitase 1, soluble citrate hydro-lyase iron-responsive element-binding protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010807715.2	XP_010806017.1	cytoplasmic aconitate hydratase isoform X2
XM_005209852.4	XP_005209909.1	cytoplasmic aconitate hydratase isoform X1
XM_024995652.1	XP_024851420.1	cytoplasmic aconitate hydratase isoform X2
XM_005209852.3	XP_005209909.1	cytoplasmic aconitate hydratase isoform X1
NM_001075591.1	NP_001069059.1	cytoplasmic aconitate hydratase

Pathways from BioSystems

Homologies

Homo sapiens (human)	ACO1	NP_002188.1
Pan troglodytes (chimpanzee)	ACO1	XP_001156102.1
Drosophila melanogaster (fruit fly)	Irp-1A	NP_477371.1
Arabidopsis thaliana (thale cress)	ACO1	NP_195308.1
	ACO1	XP_001103761.1
Bos taurus (cattle)	ACO1	NP_001069059.1
Arabidopsis thaliana (thale cress)	ACO3	NP_178634.2
Caenorhabditis elegans (roundworm)	aco-1	NP_509898.1
Oryza sativa (rice)	Os03g0136900	NP_001048898.1
Canis lupus familiaris (dog)	ACO1	XP_538698.2
Rattus norvegicus (Norway rat)	Aco1	NP_059017.1
Danio rerio (zebrafish)	aco1	NP_001030155.1
Drosophila melanogaster (fruit fly)	Irp-1B	NP_524303.2
Xenopus tropicalis (tropical clawed frog)	aco1	NP_001135643.1
Mus musculus (house mouse)	Aco1	NP_031412.2
Gallus gallus (chicken)	ACO1	NP_001025707.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007258	XP_308544.4

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Cellular regulation of the iron-responsive element binding protein: disassembly of the cubane iron-sulfur cluster results in high-affinity RNA binding.
Haile DJ, Rouault TA, Harford JB, Kennedy MC, Blondin GA, Beinert H, Klausner RD
Proceedings of the National Academy of Sciences of the United States of America89(24)11735-9(1992 Dec)

Purification and characterization of cytosolic aconitase from beef liver and its relationship to the iron-responsive element binding protein.
Kennedy MC, Mende-Mueller L, Blondin GA, Beinert H
Proceedings of the National Academy of Sciences of the United States of America89(24)11730-4(1992 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa42554	NM_001075591.1 Latest version!	Bos taurus aconitase 1 (ACO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa21863	XM_005209852.4 Latest version!	Bos taurus aconitase 1 (ACO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa48521	XM_010807715.2 Latest version!	Bos taurus aconitase 1 (ACO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa48521	XM_024995652.1 Latest version!	Bos taurus aconitase 1 (ACO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
OBa21863	XM_005209852.3	Bos taurus aconitase 1 (ACO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACO1 ( NM_001075591.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001069059.1 Bos taurus aconitase 1 (ACO1), mRNA.

Price & Availability↑

CloneID	OBa42554
Clone ID Related Accession (Same CDS sequence)	NM_001075591.1
Accession Version	NM_001075591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	cytoplasmic aconitate hydratase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC120006.1. On Oct 4, 2006 this sequence version replaced XM_590607.2. ##Evidence-Data-START## Transcript exon combination :: BC120006.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAGCAACC CATTTGCACA CCTTGTTGAG CCTTTGGATC CTGCACAACC AGGAAAGAAG 
TTCTTCAATT TGAATAAATT GGAGGATTCC AGATATGGAT CCTTACCATT TTCTATCAGA 
GTTCTCCTGG AGGCAGCCAT TCGGAACTGT GATCAATTTT TGGTGAAGAA AAATGATGTT 
GAAAATATCC TGAATTGGAA GGTCATGCAG CATAAGAACA TAGAAGTGCC CTTTAAGCCT 
GCCCGAGTCA TCCTGCAGGA CTTTACGGGT GTGCCAGCTG TGGTTGACTT TGCTGCCATG 
CGTGATGCTG TGAAAAAGTT AGGAGGCAAT CCAGAGAAAA TAAACCCCAT CTGTCCTGCT 
GATCTTGTCA TTGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTAAAG 
AAGAATCAAG ACTTGGAATT TGAAAGAAAT AAAGAACGAT TTGAATTTTT AAAGTGGGGC 
TCTCAGGCCT TTCACAACAT GAGGATTATT CCACCGGGCT CAGGAATCAT CCACCAGGTG 
AATCTGGAGT ATTTGGCAAG AGTGGTCTTT GATCAGGATG GATATTACTA CCCAGACAGC 
CTTGTGGGCA CAGATTCGCA CACCACCATG ATTGATGGTT TGGGTGTCCT TGGTTGGGGT 
GTGGGTGGTA TCGAAGCAGA AGCTGTGATG CTGGGCCAGC CAATCAGCAT GGTGCTTCCC 
CAGGTGATCG GCTACAGGCT GGTGGGAAAT CCCCACCCCC TGGTCACATC CACTGACATC 
GTGCTCACCA TCACCAAGCA CCTCCGCCAG GTTGGGGTAG TAGGCAAGTT TGTCGAGTTC 
TTTGGGCCAG GAGTAGCCCA ACTGTCCATT GCCGACCGAG CTACAATTGC CAACATGTGT 
CCAGAGTATG GGGCCACTGC CGCCTTTTTC CCAGTTGATG AAGTTAGTAT CAAGTACCTG 
GTGCAGACAG GTCGTGACAA AGAAAAAGTA AAGCATATTA AACAGTATCT TCAGGCTGTA 
GGAATGTTTC GGGACTTCAG TGACTCCTCT CAAGACCCAG ACTTTGCCCA GGTTGTGGAA 
TTAGACTTGA AAACAGTAGT GCCTTGCTGC AGCGGACCCA AAAGGCCTCA GGACAAAGTT 
GCCGTGTCTG ATATGAAAAA GGACTTTGAG AGCTGCCTGG GGGCCAAGCA AGGATTTAAA 
GGATTCCAGG TTGCTCCAGA CCATCATAAT GACCATAAGA CATTTATCTA TAATAACAGC 
AAGTTCACCC TTGCTCATGG TTCTGTGGTA ATCGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCAT CTGTGATGTT AGGGGCAGGC TTGTTAGCGA AGAAGGCTGT GGATGCAGGC 
CTGAGCGTGA AGCCTTACAT CAAAACTAGC CTGTCTCCTG GCAGTGGTGT GGTCACCTAC 
TACCTGCGGG AAAGCGGAGT CATGCCTTAC CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT CGGCAACAGC GGGCCCTTAC CGGAAGCTGT GGTAGAAGCC 
ATCGTACAGG GGGACCTCGT AGCTGTTGGA GTACTGTCTG GGAACAGGAA CTTTGAAGGG 
CGAGTCCACC CCAATACCCG GGCCAACTAT TTAGCCTCTC CGCCCTTAGT AATAGCCTAT 
GCGATTGCTG GGACCATCAG GATCGACTTT GAGAAAGAGC CACTAGGTGT CAATGCCAAG 
GGACAGCAAG TATTTCTGAA AGATATCTGG CCGACCAGAG ATGAGATCCA GGCAGTGGAA 
CGTCAGTACG TGATCCCTGG GATGTTTAAG GAGGTCTACC AGAAAATAGA GACTGTGAAT 
GAGAGCTGGA ATGCCTTAGC AGCCCCGTCA GATAAGCTAT ATTGCTGGAA TCCCAAATCT 
ACATACATTA AATCACCACC ATTCTTTGAA GACCTGACTT TGGATCTTCA GCCCCCTAAG 
TCTATTGTGG ATGCCTATGT GCTATTAAAC TTGGGAGATT CAGTAACAAC AGACCACATC 
TCTCCAGCTG GAAACATCGC AAGAAACAGC CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTGACTCCTC GAGAATTCAA CTCCTACGGC TCCCGCCGAG GGAATGATGC CATCATGGCA 
CGGGGGACAT TTGCCAACAT CCGCTTATTA AACAAATTCT TGAACAAGCA GGCACCGCAG 
ACTATCCACC TGCCTTCTGG TGAAATTCTT GACGTGTTCG ACGCCGCTGA GCGGTACCAG 
CAGGCGGGCC TCCCCCTCAT CGTCCTGGCG GGCAAAGAGT ACGGCTCGGG CAGCTCCCGA 
GACTGGGCCG CCAAGGGCCC TTTCCTCCTG GGAATCAGAG CTGTCCTAGC AGAGAGTTAC 
GAGCGCATTC ACCGAAGTAA CTTGGTCGGG ATGGGGGTGA TCCCTCTCGA GTACCTCCCT 
GGGGAGAATG CAGACACCCT GGGACTCACT GGGCGTGAAC GATACACTAT TAGCATTCCA 
GAAACCCTGA AACCACGAAT GAAAGTCCAG ATCAAGCTGG ATACCGGGAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC CGACGTGGAG CTCACTTATT TCCATAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GACCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001069059.1
CDS	91..2760
Translation	MSNPFAHLVEPLDPAQPGKKFFNLNKLEDSRYGSLPFSIRVLLEAAIRNCDQFLVKKNDVENILNWKVMQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGNPEKINPICPADLVIDHSIQVDFNRRADSLKKNQDLEFERNKERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGVLGWGVGGIEAEAVMLGQPISMVLPQVIGYRLVGNPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSIKYLVQTGRDKEKVKHIKQYLQAVGMFRDFSDSSQDPDFAQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPDHHNDHKTFIYNNSKFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLSVKPYIKTSLSPGSGVVTYYLRESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEAVVEAIVQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALAAPSDKLYCWNPKSTYIKSPPFFEDLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAIMARGTFANIRLLNKFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGSGSSRDWAAKGPFLLGIRAVLAESYERIHRSNLVGMGVIPLEYLPGENADTLGLTGRERYTISIPETLKPRMKVQIKLDTGKTFQAVMRFDTDVELTYFHNGGILNYMIRKMTK

Target ORF information:

RefSeq Version	NM_001075591.1
Organism	Bos taurus(cattle)
Definition	Bos taurus aconitase 1 (ACO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075591.1

ORF Insert Sequence:

ATGAGCAACC CATTTGCACA CCTTGTTGAG CCTTTGGATC CTGCACAACC AGGAAAGAAG 
TTCTTCAATT TGAATAAATT GGAGGATTCC AGATATGGAT CCTTACCATT TTCTATCAGA 
GTTCTCCTGG AGGCAGCCAT TCGGAACTGT GATCAATTTT TGGTGAAGAA AAATGATGTT 
GAAAATATCC TGAATTGGAA GGTCATGCAG CATAAGAACA TAGAAGTGCC CTTTAAGCCT 
GCCCGAGTCA TCCTGCAGGA CTTTACGGGT GTGCCAGCTG TGGTTGACTT TGCTGCCATG 
CGTGATGCTG TGAAAAAGTT AGGAGGCAAT CCAGAGAAAA TAAACCCCAT CTGTCCTGCT 
GATCTTGTCA TTGATCATTC CATCCAGGTT GATTTCAACA GAAGGGCAGA CAGTTTAAAG 
AAGAATCAAG ACTTGGAATT TGAAAGAAAT AAAGAACGAT TTGAATTTTT AAAGTGGGGC 
TCTCAGGCCT TTCACAACAT GAGGATTATT CCACCGGGCT CAGGAATCAT CCACCAGGTG 
AATCTGGAGT ATTTGGCAAG AGTGGTCTTT GATCAGGATG GATATTACTA CCCAGACAGC 
CTTGTGGGCA CAGATTCGCA CACCACCATG ATTGATGGTT TGGGTGTCCT TGGTTGGGGT 
GTGGGTGGTA TCGAAGCAGA AGCTGTGATG CTGGGCCAGC CAATCAGCAT GGTGCTTCCC 
CAGGTGATCG GCTACAGGCT GGTGGGAAAT CCCCACCCCC TGGTCACATC CACTGACATC 
GTGCTCACCA TCACCAAGCA CCTCCGCCAG GTTGGGGTAG TAGGCAAGTT TGTCGAGTTC 
TTTGGGCCAG GAGTAGCCCA ACTGTCCATT GCCGACCGAG CTACAATTGC CAACATGTGT 
CCAGAGTATG GGGCCACTGC CGCCTTTTTC CCAGTTGATG AAGTTAGTAT CAAGTACCTG 
GTGCAGACAG GTCGTGACAA AGAAAAAGTA AAGCATATTA AACAGTATCT TCAGGCTGTA 
GGAATGTTTC GGGACTTCAG TGACTCCTCT CAAGACCCAG ACTTTGCCCA GGTTGTGGAA 
TTAGACTTGA AAACAGTAGT GCCTTGCTGC AGCGGACCCA AAAGGCCTCA GGACAAAGTT 
GCCGTGTCTG ATATGAAAAA GGACTTTGAG AGCTGCCTGG GGGCCAAGCA AGGATTTAAA 
GGATTCCAGG TTGCTCCAGA CCATCATAAT GACCATAAGA CATTTATCTA TAATAACAGC 
AAGTTCACCC TTGCTCATGG TTCTGTGGTA ATCGCTGCCA TTACTAGCTG CACAAACACC 
AGTAATCCAT CTGTGATGTT AGGGGCAGGC TTGTTAGCGA AGAAGGCTGT GGATGCAGGC 
CTGAGCGTGA AGCCTTACAT CAAAACTAGC CTGTCTCCTG GCAGTGGTGT GGTCACCTAC 
TACCTGCGGG AAAGCGGAGT CATGCCTTAC CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC 
TATGGCTGCA TGACCTGCAT CGGCAACAGC GGGCCCTTAC CGGAAGCTGT GGTAGAAGCC 
ATCGTACAGG GGGACCTCGT AGCTGTTGGA GTACTGTCTG GGAACAGGAA CTTTGAAGGG 
CGAGTCCACC CCAATACCCG GGCCAACTAT TTAGCCTCTC CGCCCTTAGT AATAGCCTAT 
GCGATTGCTG GGACCATCAG GATCGACTTT GAGAAAGAGC CACTAGGTGT CAATGCCAAG 
GGACAGCAAG TATTTCTGAA AGATATCTGG CCGACCAGAG ATGAGATCCA GGCAGTGGAA 
CGTCAGTACG TGATCCCTGG GATGTTTAAG GAGGTCTACC AGAAAATAGA GACTGTGAAT 
GAGAGCTGGA ATGCCTTAGC AGCCCCGTCA GATAAGCTAT ATTGCTGGAA TCCCAAATCT 
ACATACATTA AATCACCACC ATTCTTTGAA GACCTGACTT TGGATCTTCA GCCCCCTAAG 
TCTATTGTGG ATGCCTATGT GCTATTAAAC TTGGGAGATT CAGTAACAAC AGACCACATC 
TCTCCAGCTG GAAACATCGC AAGAAACAGC CCTGCTGCTC GCTACTTAAC TAACAGAGGC 
CTGACTCCTC GAGAATTCAA CTCCTACGGC TCCCGCCGAG GGAATGATGC CATCATGGCA 
CGGGGGACAT TTGCCAACAT CCGCTTATTA AACAAATTCT TGAACAAGCA GGCACCGCAG 
ACTATCCACC TGCCTTCTGG TGAAATTCTT GACGTGTTCG ACGCCGCTGA GCGGTACCAG 
CAGGCGGGCC TCCCCCTCAT CGTCCTGGCG GGCAAAGAGT ACGGCTCGGG CAGCTCCCGA 
GACTGGGCCG CCAAGGGCCC TTTCCTCCTG GGAATCAGAG CTGTCCTAGC AGAGAGTTAC 
GAGCGCATTC ACCGAAGTAA CTTGGTCGGG ATGGGGGTGA TCCCTCTCGA GTACCTCCCT 
GGGGAGAATG CAGACACCCT GGGACTCACT GGGCGTGAAC GATACACTAT TAGCATTCCA 
GAAACCCTGA AACCACGAAT GAAAGTCCAG ATCAAGCTGG ATACCGGGAA GACCTTCCAG 
GCTGTCATGA GGTTTGACAC CGACGTGGAG CTCACTTATT TCCATAACGG GGGCATCCTC 
AACTACATGA TCCGCAAGAT GACCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACO1 ( XM_005209852.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209909.1 Bos taurus aconitase 1 (ACO1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa21863
Clone ID Related Accession (Same CDS sequence)	XM_005209852.4 , XM_005209852.3
Accession Version	XM_005209852.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cytoplasmic aconitate hydratase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209852.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGGG ACACCTGGTC ATCAGGAATC ATGAGCAACC CATTTGCACA CCTTGTTGAG 
CCTTTGGATC CTGCACAACC AGGAAAGAAG TTCTTCAATT TGAATAAATT GGAGGATTCC 
AGATATGGAT CCTTACCATT TTCTATCAGA GTTCTCCTGG AGGCAGCCAT TCGGAACTGT 
GATCAATTTT TGGTGAAGAA AAATGATGTT GAAAATATCC TGAATTGGAA GGTCATGCAG 
CATAAGAACA TAGAAGTGCC CTTTAAGCCT GCCCGAGTCA TCCTGCAGGA CTTTACGGGT 
GTGCCAGCTG TGGTTGACTT TGCTGCCATG CGTGATGCTG TGAAAAAGTT AGGAGGCAAT 
CCAGAGAAAA TAAACCCCAT CTGTCCTGCT GATCTTGTCA TTGATCATTC CATCCAGGTT 
GATTTCAACA GAAGGGCAGA CAGTTTAAAG AAGAATCAAG ACTTGGAATT TGAAAGAAAT 
AAAGAACGAT TTGAATTTTT AAAGTGGGGC TCTCAGGCCT TTCACAACAT GAGGATTATT 
CCACCGGGCT CAGGAATCAT CCACCAGGTG AATCTGGAGT ATTTGGCAAG AGTGGTCTTT 
GATCAGGATG GATATTACTA CCCAGACAGC CTTGTGGGCA CAGATTCGCA CACCACCATG 
ATTGATGGTT TGGGTGTCCT TGGTTGGGGT GTGGGTGGTA TCGAAGCAGA AGCTGTGATG 
CTGGGCCAGC CAATCAGCAT GGTGCTTCCC CAGGTGATCG GCTACAGGCT GGTGGGAAAT 
CCCCACCCCC TGGTCACATC CACTGACATC GTGCTCACCA TCACCAAGCA CCTCCGCCAG 
GTTGGGGTAG TAGGCAAGTT TGTCGAGTTC TTTGGGCCAG GAGTAGCCCA ACTGTCCATT 
GCCGACCGAG CTACAATTGC CAACATGTGT CCAGAGTATG GGGCCACTGC CGCCTTTTTC 
CCAGTTGATG AAGTTAGTAT CAAGTACCTG GTGCAGACAG GTCGTGACAA AGAAAAAGTA 
AAGCATATTA AACAGTATCT TCAGGTTGTA GGAATGTTTC GGGACTTCAG TGACTCCTCT 
CAAGACCCAG ACTTTGCCCA GGTTGTGGAA TTAGACTTGA AAACAGTAGT GCCTTGCTGC 
AGCGGACCCA AAAGGCCTCA GGACAAAGTT GCCGTGTCTG ATATGAAAAA GGACTTTGAG 
AGCTGCCTGG GGGCCAAGCA AGGATTTAAA GGATTCCAGG TTGCTCCAGA CCATCATAAT 
GACCATAAGA CATTTATCTA TAATAACAGC AAGTTCACCC TTGCTCATGG TTCTGTGGTA 
ATCGCTGCCA TTACTAGCTG CACAAACACC AGTAATCCAT CTGTGATGTT AGGGGCAGGC 
TTGTTAGCGA AGAAGGCTGT GGATGCAGGC CTGAGCGTGA AGCCTTACAT CAAAACTAGC 
CTGTCTCCTG GCAGTGGTGT GGTCACCTAC TACCTGCGGG AAAGCGGAGT CATGCCTTAC 
CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC TATGGCTGCA TGACCTGCAT CGGCAACAGC 
GGGCCCTTAC CGGAAGCTGT GGTAGAAGCC ATCGTACAGG GGGACCTCGT AGCTGTTGGA 
GTACTGTCTG GGAACAGGAA CTTTGAAGGG CGAGTCCACC CCAATACCCG GGCCAACTAT 
TTAGCCTCTC CGCCCTTAGT AATAGCCTAT GCGATTGCTG GGACCATCAG GATCGACTTT 
GAGAAAGAGC CACTAGGTGT CAATGCCAAG GGACAGCAAG TATTTCTGAA AGATATCTGG 
CCGACCAGAG ATGAGATCCA GGCAGTGGAA CGTCAGTACG TGATCCCTGG GATGTTTAAG 
GAGGTCTACC AGAAAATAGA GACTGTGAAT GAGAGCTGGA ATGCCTTAGC AGCCCCGTCA 
GATAAGCTAT ATTGCTGGAA TCCCAAATCT ACATACATTA AATCACCACC ATTCTTTGAA 
GACCTGACTT TGGATCTTCA GCCCCCTAAG TCTATTGTGG ATGCCTATGT GCTATTAAAC 
TTGGGAGATT CAGTAACAAC AGACCACATC TCTCCAGCTG GAAACATCGC AAGAAACAGC 
CCTGCTGCTC GCTACTTAAC TAACAGAGGC CTGACTCCTC GAGAATTCAA CTCCTACGGC 
TCCCGCCGAG GGAATGATGC CATCATGGCA CGGGGGACAT TTGCCAACAT CCGCTTATTA 
AACAAATTCT TGAACAAGCA GGCACCGCAG ACTATCCACC TGCCTTCTGG TGAAATTCTT 
GACGTGTTCG ACGCCGCTGA GCGGTACCAG CAGGCGGGCC TCCCCCTCAT CGTCCTGGCG 
GGCAAAGAGT ACGGCTCGGG CAGCTCCCGA GACTGGGCCG CCAAGGGCCC TTTCCTCCTG 
GGAATCAGAG CTGTCCTAGC AGAGAGTTAC GAGCGCATTC ACCGAAGTAA CTTGGTCGGG 
ATGGGGGTGA TCCCTCTCGA GTACCTCCCT GGGGAGAATG CAGACACCCT GGGACTCACT 
GGGCGTGAAC GATACACTAT TAGCATTCCA GAAACCCTGA AACCACGAAT GAAAGTCCAG 
ATCAAGCTGG ATACCGGGAA GACCTTCCAG GCTGTCATGA GGTTTGACAC CGACGTGGAG 
CTCACTTATT TCCATAACGG GGGCATCCTC AACTACATGA TCCGCAAGAT GACCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209909.1
CDS	228..2927
Translation	MARDTWSSGIMSNPFAHLVEPLDPAQPGKKFFNLNKLEDSRYGSLPFSIRVLLEAAIRNCDQFLVKKNDVENILNWKVMQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGNPEKINPICPADLVIDHSIQVDFNRRADSLKKNQDLEFERNKERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGVLGWGVGGIEAEAVMLGQPISMVLPQVIGYRLVGNPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSIKYLVQTGRDKEKVKHIKQYLQVVGMFRDFSDSSQDPDFAQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPDHHNDHKTFIYNNSKFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLSVKPYIKTSLSPGSGVVTYYLRESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEAVVEAIVQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALAAPSDKLYCWNPKSTYIKSPPFFEDLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAIMARGTFANIRLLNKFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGSGSSRDWAAKGPFLLGIRAVLAESYERIHRSNLVGMGVIPLEYLPGENADTLGLTGRERYTISIPETLKPRMKVQIKLDTGKTFQAVMRFDTDVELTYFHNGGILNYMIRKMTK

Target ORF information:

RefSeq Version	XM_005209852.4
Organism	Bos taurus(cattle)
Definition	Bos taurus aconitase 1 (ACO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209852.4

ORF Insert Sequence:

ATGGCGAGGG ACACCTGGTC ATCAGGAATC ATGAGCAACC CATTTGCACA CCTTGTTGAG 
CCTTTGGATC CTGCACAACC AGGAAAGAAG TTCTTCAATT TGAATAAATT GGAGGATTCC 
AGATATGGAT CCTTACCATT TTCTATCAGA GTTCTCCTGG AGGCAGCCAT TCGGAACTGT 
GATCAATTTT TGGTGAAGAA AAATGATGTT GAAAATATCC TGAATTGGAA GGTCATGCAG 
CATAAGAACA TAGAAGTGCC CTTTAAGCCT GCCCGAGTCA TCCTGCAGGA CTTTACGGGT 
GTGCCAGCTG TGGTTGACTT TGCTGCCATG CGTGATGCTG TGAAAAAGTT AGGAGGCAAT 
CCAGAGAAAA TAAACCCCAT CTGTCCTGCT GATCTTGTCA TTGATCATTC CATCCAGGTT 
GATTTCAACA GAAGGGCAGA CAGTTTAAAG AAGAATCAAG ACTTGGAATT TGAAAGAAAT 
AAAGAACGAT TTGAATTTTT AAAGTGGGGC TCTCAGGCCT TTCACAACAT GAGGATTATT 
CCACCGGGCT CAGGAATCAT CCACCAGGTG AATCTGGAGT ATTTGGCAAG AGTGGTCTTT 
GATCAGGATG GATATTACTA CCCAGACAGC CTTGTGGGCA CAGATTCGCA CACCACCATG 
ATTGATGGTT TGGGTGTCCT TGGTTGGGGT GTGGGTGGTA TCGAAGCAGA AGCTGTGATG 
CTGGGCCAGC CAATCAGCAT GGTGCTTCCC CAGGTGATCG GCTACAGGCT GGTGGGAAAT 
CCCCACCCCC TGGTCACATC CACTGACATC GTGCTCACCA TCACCAAGCA CCTCCGCCAG 
GTTGGGGTAG TAGGCAAGTT TGTCGAGTTC TTTGGGCCAG GAGTAGCCCA ACTGTCCATT 
GCCGACCGAG CTACAATTGC CAACATGTGT CCAGAGTATG GGGCCACTGC CGCCTTTTTC 
CCAGTTGATG AAGTTAGTAT CAAGTACCTG GTGCAGACAG GTCGTGACAA AGAAAAAGTA 
AAGCATATTA AACAGTATCT TCAGGTTGTA GGAATGTTTC GGGACTTCAG TGACTCCTCT 
CAAGACCCAG ACTTTGCCCA GGTTGTGGAA TTAGACTTGA AAACAGTAGT GCCTTGCTGC 
AGCGGACCCA AAAGGCCTCA GGACAAAGTT GCCGTGTCTG ATATGAAAAA GGACTTTGAG 
AGCTGCCTGG GGGCCAAGCA AGGATTTAAA GGATTCCAGG TTGCTCCAGA CCATCATAAT 
GACCATAAGA CATTTATCTA TAATAACAGC AAGTTCACCC TTGCTCATGG TTCTGTGGTA 
ATCGCTGCCA TTACTAGCTG CACAAACACC AGTAATCCAT CTGTGATGTT AGGGGCAGGC 
TTGTTAGCGA AGAAGGCTGT GGATGCAGGC CTGAGCGTGA AGCCTTACAT CAAAACTAGC 
CTGTCTCCTG GCAGTGGTGT GGTCACCTAC TACCTGCGGG AAAGCGGAGT CATGCCTTAC 
CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC TATGGCTGCA TGACCTGCAT CGGCAACAGC 
GGGCCCTTAC CGGAAGCTGT GGTAGAAGCC ATCGTACAGG GGGACCTCGT AGCTGTTGGA 
GTACTGTCTG GGAACAGGAA CTTTGAAGGG CGAGTCCACC CCAATACCCG GGCCAACTAT 
TTAGCCTCTC CGCCCTTAGT AATAGCCTAT GCGATTGCTG GGACCATCAG GATCGACTTT 
GAGAAAGAGC CACTAGGTGT CAATGCCAAG GGACAGCAAG TATTTCTGAA AGATATCTGG 
CCGACCAGAG ATGAGATCCA GGCAGTGGAA CGTCAGTACG TGATCCCTGG GATGTTTAAG 
GAGGTCTACC AGAAAATAGA GACTGTGAAT GAGAGCTGGA ATGCCTTAGC AGCCCCGTCA 
GATAAGCTAT ATTGCTGGAA TCCCAAATCT ACATACATTA AATCACCACC ATTCTTTGAA 
GACCTGACTT TGGATCTTCA GCCCCCTAAG TCTATTGTGG ATGCCTATGT GCTATTAAAC 
TTGGGAGATT CAGTAACAAC AGACCACATC TCTCCAGCTG GAAACATCGC AAGAAACAGC 
CCTGCTGCTC GCTACTTAAC TAACAGAGGC CTGACTCCTC GAGAATTCAA CTCCTACGGC 
TCCCGCCGAG GGAATGATGC CATCATGGCA CGGGGGACAT TTGCCAACAT CCGCTTATTA 
AACAAATTCT TGAACAAGCA GGCACCGCAG ACTATCCACC TGCCTTCTGG TGAAATTCTT 
GACGTGTTCG ACGCCGCTGA GCGGTACCAG CAGGCGGGCC TCCCCCTCAT CGTCCTGGCG 
GGCAAAGAGT ACGGCTCGGG CAGCTCCCGA GACTGGGCCG CCAAGGGCCC TTTCCTCCTG 
GGAATCAGAG CTGTCCTAGC AGAGAGTTAC GAGCGCATTC ACCGAAGTAA CTTGGTCGGG 
ATGGGGGTGA TCCCTCTCGA GTACCTCCCT GGGGAGAATG CAGACACCCT GGGACTCACT 
GGGCGTGAAC GATACACTAT TAGCATTCCA GAAACCCTGA AACCACGAAT GAAAGTCCAG 
ATCAAGCTGG ATACCGGGAA GACCTTCCAG GCTGTCATGA GGTTTGACAC CGACGTGGAG 
CTCACTTATT TCCATAACGG GGGCATCCTC AACTACATGA TCCGCAAGAT GACCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACO1 ( XM_005209852.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209909.1 Bos taurus aconitase 1 (ACO1), transcript variant X1, mRNA.

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CloneID	OBa21863
Clone ID Related Accession (Same CDS sequence)	XM_005209852.4 , XM_005209852.3
Accession Version	XM_005209852.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cytoplasmic aconitate hydratase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209852.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGGG ACACCTGGTC ATCAGGAATC ATGAGCAACC CATTTGCACA CCTTGTTGAG 
CCTTTGGATC CTGCACAACC AGGAAAGAAG TTCTTCAATT TGAATAAATT GGAGGATTCC 
AGATATGGAT CCTTACCATT TTCTATCAGA GTTCTCCTGG AGGCAGCCAT TCGGAACTGT 
GATCAATTTT TGGTGAAGAA AAATGATGTT GAAAATATCC TGAATTGGAA GGTCATGCAG 
CATAAGAACA TAGAAGTGCC CTTTAAGCCT GCCCGAGTCA TCCTGCAGGA CTTTACGGGT 
GTGCCAGCTG TGGTTGACTT TGCTGCCATG CGTGATGCTG TGAAAAAGTT AGGAGGCAAT 
CCAGAGAAAA TAAACCCCAT CTGTCCTGCT GATCTTGTCA TTGATCATTC CATCCAGGTT 
GATTTCAACA GAAGGGCAGA CAGTTTAAAG AAGAATCAAG ACTTGGAATT TGAAAGAAAT 
AAAGAACGAT TTGAATTTTT AAAGTGGGGC TCTCAGGCCT TTCACAACAT GAGGATTATT 
CCACCGGGCT CAGGAATCAT CCACCAGGTG AATCTGGAGT ATTTGGCAAG AGTGGTCTTT 
GATCAGGATG GATATTACTA CCCAGACAGC CTTGTGGGCA CAGATTCGCA CACCACCATG 
ATTGATGGTT TGGGTGTCCT TGGTTGGGGT GTGGGTGGTA TCGAAGCAGA AGCTGTGATG 
CTGGGCCAGC CAATCAGCAT GGTGCTTCCC CAGGTGATCG GCTACAGGCT GGTGGGAAAT 
CCCCACCCCC TGGTCACATC CACTGACATC GTGCTCACCA TCACCAAGCA CCTCCGCCAG 
GTTGGGGTAG TAGGCAAGTT TGTCGAGTTC TTTGGGCCAG GAGTAGCCCA ACTGTCCATT 
GCCGACCGAG CTACAATTGC CAACATGTGT CCAGAGTATG GGGCCACTGC CGCCTTTTTC 
CCAGTTGATG AAGTTAGTAT CAAGTACCTG GTGCAGACAG GTCGTGACAA AGAAAAAGTA 
AAGCATATTA AACAGTATCT TCAGGTTGTA GGAATGTTTC GGGACTTCAG TGACTCCTCT 
CAAGACCCAG ACTTTGCCCA GGTTGTGGAA TTAGACTTGA AAACAGTAGT GCCTTGCTGC 
AGCGGACCCA AAAGGCCTCA GGACAAAGTT GCCGTGTCTG ATATGAAAAA GGACTTTGAG 
AGCTGCCTGG GGGCCAAGCA AGGATTTAAA GGATTCCAGG TTGCTCCAGA CCATCATAAT 
GACCATAAGA CATTTATCTA TAATAACAGC AAGTTCACCC TTGCTCATGG TTCTGTGGTA 
ATCGCTGCCA TTACTAGCTG CACAAACACC AGTAATCCAT CTGTGATGTT AGGGGCAGGC 
TTGTTAGCGA AGAAGGCTGT GGATGCAGGC CTGAGCGTGA AGCCTTACAT CAAAACTAGC 
CTGTCTCCTG GCAGTGGTGT GGTCACCTAC TACCTGCGGG AAAGCGGAGT CATGCCTTAC 
CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC TATGGCTGCA TGACCTGCAT CGGCAACAGC 
GGGCCCTTAC CGGAAGCTGT GGTAGAAGCC ATCGTACAGG GGGACCTCGT AGCTGTTGGA 
GTACTGTCTG GGAACAGGAA CTTTGAAGGG CGAGTCCACC CCAATACCCG GGCCAACTAT 
TTAGCCTCTC CGCCCTTAGT AATAGCCTAT GCGATTGCTG GGACCATCAG GATCGACTTT 
GAGAAAGAGC CACTAGGTGT CAATGCCAAG GGACAGCAAG TATTTCTGAA AGATATCTGG 
CCGACCAGAG ATGAGATCCA GGCAGTGGAA CGTCAGTACG TGATCCCTGG GATGTTTAAG 
GAGGTCTACC AGAAAATAGA GACTGTGAAT GAGAGCTGGA ATGCCTTAGC AGCCCCGTCA 
GATAAGCTAT ATTGCTGGAA TCCCAAATCT ACATACATTA AATCACCACC ATTCTTTGAA 
GACCTGACTT TGGATCTTCA GCCCCCTAAG TCTATTGTGG ATGCCTATGT GCTATTAAAC 
TTGGGAGATT CAGTAACAAC AGACCACATC TCTCCAGCTG GAAACATCGC AAGAAACAGC 
CCTGCTGCTC GCTACTTAAC TAACAGAGGC CTGACTCCTC GAGAATTCAA CTCCTACGGC 
TCCCGCCGAG GGAATGATGC CATCATGGCA CGGGGGACAT TTGCCAACAT CCGCTTATTA 
AACAAATTCT TGAACAAGCA GGCACCGCAG ACTATCCACC TGCCTTCTGG TGAAATTCTT 
GACGTGTTCG ACGCCGCTGA GCGGTACCAG CAGGCGGGCC TCCCCCTCAT CGTCCTGGCG 
GGCAAAGAGT ACGGCTCGGG CAGCTCCCGA GACTGGGCCG CCAAGGGCCC TTTCCTCCTG 
GGAATCAGAG CTGTCCTAGC AGAGAGTTAC GAGCGCATTC ACCGAAGTAA CTTGGTCGGG 
ATGGGGGTGA TCCCTCTCGA GTACCTCCCT GGGGAGAATG CAGACACCCT GGGACTCACT 
GGGCGTGAAC GATACACTAT TAGCATTCCA GAAACCCTGA AACCACGAAT GAAAGTCCAG 
ATCAAGCTGG ATACCGGGAA GACCTTCCAG GCTGTCATGA GGTTTGACAC CGACGTGGAG 
CTCACTTATT TCCATAACGG GGGCATCCTC AACTACATGA TCCGCAAGAT GACCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209909.1
CDS	131..2830
Translation	MARDTWSSGIMSNPFAHLVEPLDPAQPGKKFFNLNKLEDSRYGSLPFSIRVLLEAAIRNCDQFLVKKNDVENILNWKVMQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGNPEKINPICPADLVIDHSIQVDFNRRADSLKKNQDLEFERNKERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGVLGWGVGGIEAEAVMLGQPISMVLPQVIGYRLVGNPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSIKYLVQTGRDKEKVKHIKQYLQVVGMFRDFSDSSQDPDFAQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPDHHNDHKTFIYNNSKFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLSVKPYIKTSLSPGSGVVTYYLRESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEAVVEAIVQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALAAPSDKLYCWNPKSTYIKSPPFFEDLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAIMARGTFANIRLLNKFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGSGSSRDWAAKGPFLLGIRAVLAESYERIHRSNLVGMGVIPLEYLPGENADTLGLTGRERYTISIPETLKPRMKVQIKLDTGKTFQAVMRFDTDVELTYFHNGGILNYMIRKMTK

Target ORF information:

RefSeq Version	XM_005209852.3
Organism	Bos taurus(cattle)
Definition	Bos taurus aconitase 1 (ACO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209852.3

ORF Insert Sequence:

ATGGCGAGGG ACACCTGGTC ATCAGGAATC ATGAGCAACC CATTTGCACA CCTTGTTGAG 
CCTTTGGATC CTGCACAACC AGGAAAGAAG TTCTTCAATT TGAATAAATT GGAGGATTCC 
AGATATGGAT CCTTACCATT TTCTATCAGA GTTCTCCTGG AGGCAGCCAT TCGGAACTGT 
GATCAATTTT TGGTGAAGAA AAATGATGTT GAAAATATCC TGAATTGGAA GGTCATGCAG 
CATAAGAACA TAGAAGTGCC CTTTAAGCCT GCCCGAGTCA TCCTGCAGGA CTTTACGGGT 
GTGCCAGCTG TGGTTGACTT TGCTGCCATG CGTGATGCTG TGAAAAAGTT AGGAGGCAAT 
CCAGAGAAAA TAAACCCCAT CTGTCCTGCT GATCTTGTCA TTGATCATTC CATCCAGGTT 
GATTTCAACA GAAGGGCAGA CAGTTTAAAG AAGAATCAAG ACTTGGAATT TGAAAGAAAT 
AAAGAACGAT TTGAATTTTT AAAGTGGGGC TCTCAGGCCT TTCACAACAT GAGGATTATT 
CCACCGGGCT CAGGAATCAT CCACCAGGTG AATCTGGAGT ATTTGGCAAG AGTGGTCTTT 
GATCAGGATG GATATTACTA CCCAGACAGC CTTGTGGGCA CAGATTCGCA CACCACCATG 
ATTGATGGTT TGGGTGTCCT TGGTTGGGGT GTGGGTGGTA TCGAAGCAGA AGCTGTGATG 
CTGGGCCAGC CAATCAGCAT GGTGCTTCCC CAGGTGATCG GCTACAGGCT GGTGGGAAAT 
CCCCACCCCC TGGTCACATC CACTGACATC GTGCTCACCA TCACCAAGCA CCTCCGCCAG 
GTTGGGGTAG TAGGCAAGTT TGTCGAGTTC TTTGGGCCAG GAGTAGCCCA ACTGTCCATT 
GCCGACCGAG CTACAATTGC CAACATGTGT CCAGAGTATG GGGCCACTGC CGCCTTTTTC 
CCAGTTGATG AAGTTAGTAT CAAGTACCTG GTGCAGACAG GTCGTGACAA AGAAAAAGTA 
AAGCATATTA AACAGTATCT TCAGGTTGTA GGAATGTTTC GGGACTTCAG TGACTCCTCT 
CAAGACCCAG ACTTTGCCCA GGTTGTGGAA TTAGACTTGA AAACAGTAGT GCCTTGCTGC 
AGCGGACCCA AAAGGCCTCA GGACAAAGTT GCCGTGTCTG ATATGAAAAA GGACTTTGAG 
AGCTGCCTGG GGGCCAAGCA AGGATTTAAA GGATTCCAGG TTGCTCCAGA CCATCATAAT 
GACCATAAGA CATTTATCTA TAATAACAGC AAGTTCACCC TTGCTCATGG TTCTGTGGTA 
ATCGCTGCCA TTACTAGCTG CACAAACACC AGTAATCCAT CTGTGATGTT AGGGGCAGGC 
TTGTTAGCGA AGAAGGCTGT GGATGCAGGC CTGAGCGTGA AGCCTTACAT CAAAACTAGC 
CTGTCTCCTG GCAGTGGTGT GGTCACCTAC TACCTGCGGG AAAGCGGAGT CATGCCTTAC 
CTGTCTCAGC TTGGGTTTGA CGTGGTGGGC TATGGCTGCA TGACCTGCAT CGGCAACAGC 
GGGCCCTTAC CGGAAGCTGT GGTAGAAGCC ATCGTACAGG GGGACCTCGT AGCTGTTGGA 
GTACTGTCTG GGAACAGGAA CTTTGAAGGG CGAGTCCACC CCAATACCCG GGCCAACTAT 
TTAGCCTCTC CGCCCTTAGT AATAGCCTAT GCGATTGCTG GGACCATCAG GATCGACTTT 
GAGAAAGAGC CACTAGGTGT CAATGCCAAG GGACAGCAAG TATTTCTGAA AGATATCTGG 
CCGACCAGAG ATGAGATCCA GGCAGTGGAA CGTCAGTACG TGATCCCTGG GATGTTTAAG 
GAGGTCTACC AGAAAATAGA GACTGTGAAT GAGAGCTGGA ATGCCTTAGC AGCCCCGTCA 
GATAAGCTAT ATTGCTGGAA TCCCAAATCT ACATACATTA AATCACCACC ATTCTTTGAA 
GACCTGACTT TGGATCTTCA GCCCCCTAAG TCTATTGTGG ATGCCTATGT GCTATTAAAC 
TTGGGAGATT CAGTAACAAC AGACCACATC TCTCCAGCTG GAAACATCGC AAGAAACAGC 
CCTGCTGCTC GCTACTTAAC TAACAGAGGC CTGACTCCTC GAGAATTCAA CTCCTACGGC 
TCCCGCCGAG GGAATGATGC CATCATGGCA CGGGGGACAT TTGCCAACAT CCGCTTATTA 
AACAAATTCT TGAACAAGCA GGCACCGCAG ACTATCCACC TGCCTTCTGG TGAAATTCTT 
GACGTGTTCG ACGCCGCTGA GCGGTACCAG CAGGCGGGCC TCCCCCTCAT CGTCCTGGCG 
GGCAAAGAGT ACGGCTCGGG CAGCTCCCGA GACTGGGCCG CCAAGGGCCC TTTCCTCCTG 
GGAATCAGAG CTGTCCTAGC AGAGAGTTAC GAGCGCATTC ACCGAAGTAA CTTGGTCGGG 
ATGGGGGTGA TCCCTCTCGA GTACCTCCCT GGGGAGAATG CAGACACCCT GGGACTCACT 
GGGCGTGAAC GATACACTAT TAGCATTCCA GAAACCCTGA AACCACGAAT GAAAGTCCAG 
ATCAAGCTGG ATACCGGGAA GACCTTCCAG GCTGTCATGA GGTTTGACAC CGACGTGGAG 
CTCACTTATT TCCATAACGG GGGCATCCTC AACTACATGA TCCGCAAGAT GACCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACO1 ( XM_010807715.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010806017.1 Bos taurus aconitase 1 (ACO1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa48521
Clone ID Related Accession (Same CDS sequence)	XM_010807715.2 , XM_024995652.1
Accession Version	XM_010807715.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cytoplasmic aconitate hydratase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807715.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACCT GGTCATCAGG AATCATGAGC AACCCATTTG CACACCTTGT TGAGCCTTTG 
GATCCTGCAC AACCAGGAAA GAAGTTCTTC AATTTGAATA AATTGGAGGA TTCCAGATAT 
GGATCCTTAC CATTTTCTAT CAGAGTTCTC CTGGAGGCAG CCATTCGGAA CTGTGATCAA 
TTTTTGGTGA AGAAAAATGA TGTTGAAAAT ATCCTGAATT GGAAGGTCAT GCAGCATAAG 
AACATAGAAG TGCCCTTTAA GCCTGCCCGA GTCATCCTGC AGGACTTTAC GGGTGTGCCA 
GCTGTGGTTG ACTTTGCTGC CATGCGTGAT GCTGTGAAAA AGTTAGGAGG CAATCCAGAG 
AAAATAAACC CCATCTGTCC TGCTGATCTT GTCATTGATC ATTCCATCCA GGTTGATTTC 
AACAGAAGGG CAGACAGTTT AAAGAAGAAT CAAGACTTGG AATTTGAAAG AAATAAAGAA 
CGATTTGAAT TTTTAAAGTG GGGCTCTCAG GCCTTTCACA ACATGAGGAT TATTCCACCG 
GGCTCAGGAA TCATCCACCA GGTGAATCTG GAGTATTTGG CAAGAGTGGT CTTTGATCAG 
GATGGATATT ACTACCCAGA CAGCCTTGTG GGCACAGATT CGCACACCAC CATGATTGAT 
GGTTTGGGTG TCCTTGGTTG GGGTGTGGGT GGTATCGAAG CAGAAGCTGT GATGCTGGGC 
CAGCCAATCA GCATGGTGCT TCCCCAGGTG ATCGGCTACA GGCTGGTGGG AAATCCCCAC 
CCCCTGGTCA CATCCACTGA CATCGTGCTC ACCATCACCA AGCACCTCCG CCAGGTTGGG 
GTAGTAGGCA AGTTTGTCGA GTTCTTTGGG CCAGGAGTAG CCCAACTGTC CATTGCCGAC 
CGAGCTACAA TTGCCAACAT GTGTCCAGAG TATGGGGCCA CTGCCGCCTT TTTCCCAGTT 
GATGAAGTTA GTATCAAGTA CCTGGTGCAG ACAGGTCGTG ACAAAGAAAA AGTAAAGCAT 
ATTAAACAGT ATCTTCAGGT TGTAGGAATG TTTCGGGACT TCAGTGACTC CTCTCAAGAC 
CCAGACTTTG CCCAGGTTGT GGAATTAGAC TTGAAAACAG TAGTGCCTTG CTGCAGCGGA 
CCCAAAAGGC CTCAGGACAA AGTTGCCGTG TCTGATATGA AAAAGGACTT TGAGAGCTGC 
CTGGGGGCCA AGCAAGGATT TAAAGGATTC CAGGTTGCTC CAGACCATCA TAATGACCAT 
AAGACATTTA TCTATAATAA CAGCAAGTTC ACCCTTGCTC ATGGTTCTGT GGTAATCGCT 
GCCATTACTA GCTGCACAAA CACCAGTAAT CCATCTGTGA TGTTAGGGGC AGGCTTGTTA 
GCGAAGAAGG CTGTGGATGC AGGCCTGAGC GTGAAGCCTT ACATCAAAAC TAGCCTGTCT 
CCTGGCAGTG GTGTGGTCAC CTACTACCTG CGGGAAAGCG GAGTCATGCC TTACCTGTCT 
CAGCTTGGGT TTGACGTGGT GGGCTATGGC TGCATGACCT GCATCGGCAA CAGCGGGCCC 
TTACCGGAAG CTGTGGTAGA AGCCATCGTA CAGGGGGACC TCGTAGCTGT TGGAGTACTG 
TCTGGGAACA GGAACTTTGA AGGGCGAGTC CACCCCAATA CCCGGGCCAA CTATTTAGCC 
TCTCCGCCCT TAGTAATAGC CTATGCGATT GCTGGGACCA TCAGGATCGA CTTTGAGAAA 
GAGCCACTAG GTGTCAATGC CAAGGGACAG CAAGTATTTC TGAAAGATAT CTGGCCGACC 
AGAGATGAGA TCCAGGCAGT GGAACGTCAG TACGTGATCC CTGGGATGTT TAAGGAGGTC 
TACCAGAAAA TAGAGACTGT GAATGAGAGC TGGAATGCCT TAGCAGCCCC GTCAGATAAG 
CTATATTGCT GGAATCCCAA ATCTACATAC ATTAAATCAC CACCATTCTT TGAAGACCTG 
ACTTTGGATC TTCAGCCCCC TAAGTCTATT GTGGATGCCT ATGTGCTATT AAACTTGGGA 
GATTCAGTAA CAACAGACCA CATCTCTCCA GCTGGAAACA TCGCAAGAAA CAGCCCTGCT 
GCTCGCTACT TAACTAACAG AGGCCTGACT CCTCGAGAAT TCAACTCCTA CGGCTCCCGC 
CGAGGGAATG ATGCCATCAT GGCACGGGGG ACATTTGCCA ACATCCGCTT ATTAAACAAA 
TTCTTGAACA AGCAGGCACC GCAGACTATC CACCTGCCTT CTGGTGAAAT TCTTGACGTG 
TTCGACGCCG CTGAGCGGTA CCAGCAGGCG GGCCTCCCCC TCATCGTCCT GGCGGGCAAA 
GAGTACGGCT CGGGCAGCTC CCGAGACTGG GCCGCCAAGG GCCCTTTCCT CCTGGGAATC 
AGAGCTGTCC TAGCAGAGAG TTACGAGCGC ATTCACCGAA GTAACTTGGT CGGGATGGGG 
GTGATCCCTC TCGAGTACCT CCCTGGGGAG AATGCAGACA CCCTGGGACT CACTGGGCGT 
GAACGATACA CTATTAGCAT TCCAGAAACC CTGAAACCAC GAATGAAAGT CCAGATCAAG 
CTGGATACCG GGAAGACCTT CCAGGCTGTC ATGAGGTTTG ACACCGACGT GGAGCTCACT 
TATTTCCATA ACGGGGGCAT CCTCAACTAC ATGATCCGCA AGATGACCAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010806017.1
CDS	108..2801
Translation	MDTWSSGIMSNPFAHLVEPLDPAQPGKKFFNLNKLEDSRYGSLPFSIRVLLEAAIRNCDQFLVKKNDVENILNWKVMQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGNPEKINPICPADLVIDHSIQVDFNRRADSLKKNQDLEFERNKERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGVLGWGVGGIEAEAVMLGQPISMVLPQVIGYRLVGNPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSIKYLVQTGRDKEKVKHIKQYLQVVGMFRDFSDSSQDPDFAQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPDHHNDHKTFIYNNSKFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLSVKPYIKTSLSPGSGVVTYYLRESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEAVVEAIVQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALAAPSDKLYCWNPKSTYIKSPPFFEDLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAIMARGTFANIRLLNKFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGSGSSRDWAAKGPFLLGIRAVLAESYERIHRSNLVGMGVIPLEYLPGENADTLGLTGRERYTISIPETLKPRMKVQIKLDTGKTFQAVMRFDTDVELTYFHNGGILNYMIRKMTK

Target ORF information:

RefSeq Version	XM_010807715.2
Organism	Bos taurus(cattle)
Definition	Bos taurus aconitase 1 (ACO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807715.2

ORF Insert Sequence:

ATGGACACCT GGTCATCAGG AATCATGAGC AACCCATTTG CACACCTTGT TGAGCCTTTG 
GATCCTGCAC AACCAGGAAA GAAGTTCTTC AATTTGAATA AATTGGAGGA TTCCAGATAT 
GGATCCTTAC CATTTTCTAT CAGAGTTCTC CTGGAGGCAG CCATTCGGAA CTGTGATCAA 
TTTTTGGTGA AGAAAAATGA TGTTGAAAAT ATCCTGAATT GGAAGGTCAT GCAGCATAAG 
AACATAGAAG TGCCCTTTAA GCCTGCCCGA GTCATCCTGC AGGACTTTAC GGGTGTGCCA 
GCTGTGGTTG ACTTTGCTGC CATGCGTGAT GCTGTGAAAA AGTTAGGAGG CAATCCAGAG 
AAAATAAACC CCATCTGTCC TGCTGATCTT GTCATTGATC ATTCCATCCA GGTTGATTTC 
AACAGAAGGG CAGACAGTTT AAAGAAGAAT CAAGACTTGG AATTTGAAAG AAATAAAGAA 
CGATTTGAAT TTTTAAAGTG GGGCTCTCAG GCCTTTCACA ACATGAGGAT TATTCCACCG 
GGCTCAGGAA TCATCCACCA GGTGAATCTG GAGTATTTGG CAAGAGTGGT CTTTGATCAG 
GATGGATATT ACTACCCAGA CAGCCTTGTG GGCACAGATT CGCACACCAC CATGATTGAT 
GGTTTGGGTG TCCTTGGTTG GGGTGTGGGT GGTATCGAAG CAGAAGCTGT GATGCTGGGC 
CAGCCAATCA GCATGGTGCT TCCCCAGGTG ATCGGCTACA GGCTGGTGGG AAATCCCCAC 
CCCCTGGTCA CATCCACTGA CATCGTGCTC ACCATCACCA AGCACCTCCG CCAGGTTGGG 
GTAGTAGGCA AGTTTGTCGA GTTCTTTGGG CCAGGAGTAG CCCAACTGTC CATTGCCGAC 
CGAGCTACAA TTGCCAACAT GTGTCCAGAG TATGGGGCCA CTGCCGCCTT TTTCCCAGTT 
GATGAAGTTA GTATCAAGTA CCTGGTGCAG ACAGGTCGTG ACAAAGAAAA AGTAAAGCAT 
ATTAAACAGT ATCTTCAGGT TGTAGGAATG TTTCGGGACT TCAGTGACTC CTCTCAAGAC 
CCAGACTTTG CCCAGGTTGT GGAATTAGAC TTGAAAACAG TAGTGCCTTG CTGCAGCGGA 
CCCAAAAGGC CTCAGGACAA AGTTGCCGTG TCTGATATGA AAAAGGACTT TGAGAGCTGC 
CTGGGGGCCA AGCAAGGATT TAAAGGATTC CAGGTTGCTC CAGACCATCA TAATGACCAT 
AAGACATTTA TCTATAATAA CAGCAAGTTC ACCCTTGCTC ATGGTTCTGT GGTAATCGCT 
GCCATTACTA GCTGCACAAA CACCAGTAAT CCATCTGTGA TGTTAGGGGC AGGCTTGTTA 
GCGAAGAAGG CTGTGGATGC AGGCCTGAGC GTGAAGCCTT ACATCAAAAC TAGCCTGTCT 
CCTGGCAGTG GTGTGGTCAC CTACTACCTG CGGGAAAGCG GAGTCATGCC TTACCTGTCT 
CAGCTTGGGT TTGACGTGGT GGGCTATGGC TGCATGACCT GCATCGGCAA CAGCGGGCCC 
TTACCGGAAG CTGTGGTAGA AGCCATCGTA CAGGGGGACC TCGTAGCTGT TGGAGTACTG 
TCTGGGAACA GGAACTTTGA AGGGCGAGTC CACCCCAATA CCCGGGCCAA CTATTTAGCC 
TCTCCGCCCT TAGTAATAGC CTATGCGATT GCTGGGACCA TCAGGATCGA CTTTGAGAAA 
GAGCCACTAG GTGTCAATGC CAAGGGACAG CAAGTATTTC TGAAAGATAT CTGGCCGACC 
AGAGATGAGA TCCAGGCAGT GGAACGTCAG TACGTGATCC CTGGGATGTT TAAGGAGGTC 
TACCAGAAAA TAGAGACTGT GAATGAGAGC TGGAATGCCT TAGCAGCCCC GTCAGATAAG 
CTATATTGCT GGAATCCCAA ATCTACATAC ATTAAATCAC CACCATTCTT TGAAGACCTG 
ACTTTGGATC TTCAGCCCCC TAAGTCTATT GTGGATGCCT ATGTGCTATT AAACTTGGGA 
GATTCAGTAA CAACAGACCA CATCTCTCCA GCTGGAAACA TCGCAAGAAA CAGCCCTGCT 
GCTCGCTACT TAACTAACAG AGGCCTGACT CCTCGAGAAT TCAACTCCTA CGGCTCCCGC 
CGAGGGAATG ATGCCATCAT GGCACGGGGG ACATTTGCCA ACATCCGCTT ATTAAACAAA 
TTCTTGAACA AGCAGGCACC GCAGACTATC CACCTGCCTT CTGGTGAAAT TCTTGACGTG 
TTCGACGCCG CTGAGCGGTA CCAGCAGGCG GGCCTCCCCC TCATCGTCCT GGCGGGCAAA 
GAGTACGGCT CGGGCAGCTC CCGAGACTGG GCCGCCAAGG GCCCTTTCCT CCTGGGAATC 
AGAGCTGTCC TAGCAGAGAG TTACGAGCGC ATTCACCGAA GTAACTTGGT CGGGATGGGG 
GTGATCCCTC TCGAGTACCT CCCTGGGGAG AATGCAGACA CCCTGGGACT CACTGGGCGT 
GAACGATACA CTATTAGCAT TCCAGAAACC CTGAAACCAC GAATGAAAGT CCAGATCAAG 
CTGGATACCG GGAAGACCTT CCAGGCTGTC ATGAGGTTTG ACACCGACGT GGAGCTCACT 
TATTTCCATA ACGGGGGCAT CCTCAACTAC ATGATCCGCA AGATGACCAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACO1 ( XM_024995652.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851420.1 Bos taurus aconitase 1 (ACO1), transcript variant X2, mRNA.

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CloneID	OBa48521
Clone ID Related Accession (Same CDS sequence)	XM_010807715.2 , XM_024995652.1
Accession Version	XM_024995652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cytoplasmic aconitate hydratase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACCT GGTCATCAGG AATCATGAGC AACCCATTTG CACACCTTGT TGAGCCTTTG 
GATCCTGCAC AACCAGGAAA GAAGTTCTTC AATTTGAATA AATTGGAGGA TTCCAGATAT 
GGATCCTTAC CATTTTCTAT CAGAGTTCTC CTGGAGGCAG CCATTCGGAA CTGTGATCAA 
TTTTTGGTGA AGAAAAATGA TGTTGAAAAT ATCCTGAATT GGAAGGTCAT GCAGCATAAG 
AACATAGAAG TGCCCTTTAA GCCTGCCCGA GTCATCCTGC AGGACTTTAC GGGTGTGCCA 
GCTGTGGTTG ACTTTGCTGC CATGCGTGAT GCTGTGAAAA AGTTAGGAGG CAATCCAGAG 
AAAATAAACC CCATCTGTCC TGCTGATCTT GTCATTGATC ATTCCATCCA GGTTGATTTC 
AACAGAAGGG CAGACAGTTT AAAGAAGAAT CAAGACTTGG AATTTGAAAG AAATAAAGAA 
CGATTTGAAT TTTTAAAGTG GGGCTCTCAG GCCTTTCACA ACATGAGGAT TATTCCACCG 
GGCTCAGGAA TCATCCACCA GGTGAATCTG GAGTATTTGG CAAGAGTGGT CTTTGATCAG 
GATGGATATT ACTACCCAGA CAGCCTTGTG GGCACAGATT CGCACACCAC CATGATTGAT 
GGTTTGGGTG TCCTTGGTTG GGGTGTGGGT GGTATCGAAG CAGAAGCTGT GATGCTGGGC 
CAGCCAATCA GCATGGTGCT TCCCCAGGTG ATCGGCTACA GGCTGGTGGG AAATCCCCAC 
CCCCTGGTCA CATCCACTGA CATCGTGCTC ACCATCACCA AGCACCTCCG CCAGGTTGGG 
GTAGTAGGCA AGTTTGTCGA GTTCTTTGGG CCAGGAGTAG CCCAACTGTC CATTGCCGAC 
CGAGCTACAA TTGCCAACAT GTGTCCAGAG TATGGGGCCA CTGCCGCCTT TTTCCCAGTT 
GATGAAGTTA GTATCAAGTA CCTGGTGCAG ACAGGTCGTG ACAAAGAAAA AGTAAAGCAT 
ATTAAACAGT ATCTTCAGGT TGTAGGAATG TTTCGGGACT TCAGTGACTC CTCTCAAGAC 
CCAGACTTTG CCCAGGTTGT GGAATTAGAC TTGAAAACAG TAGTGCCTTG CTGCAGCGGA 
CCCAAAAGGC CTCAGGACAA AGTTGCCGTG TCTGATATGA AAAAGGACTT TGAGAGCTGC 
CTGGGGGCCA AGCAAGGATT TAAAGGATTC CAGGTTGCTC CAGACCATCA TAATGACCAT 
AAGACATTTA TCTATAATAA CAGCAAGTTC ACCCTTGCTC ATGGTTCTGT GGTAATCGCT 
GCCATTACTA GCTGCACAAA CACCAGTAAT CCATCTGTGA TGTTAGGGGC AGGCTTGTTA 
GCGAAGAAGG CTGTGGATGC AGGCCTGAGC GTGAAGCCTT ACATCAAAAC TAGCCTGTCT 
CCTGGCAGTG GTGTGGTCAC CTACTACCTG CGGGAAAGCG GAGTCATGCC TTACCTGTCT 
CAGCTTGGGT TTGACGTGGT GGGCTATGGC TGCATGACCT GCATCGGCAA CAGCGGGCCC 
TTACCGGAAG CTGTGGTAGA AGCCATCGTA CAGGGGGACC TCGTAGCTGT TGGAGTACTG 
TCTGGGAACA GGAACTTTGA AGGGCGAGTC CACCCCAATA CCCGGGCCAA CTATTTAGCC 
TCTCCGCCCT TAGTAATAGC CTATGCGATT GCTGGGACCA TCAGGATCGA CTTTGAGAAA 
GAGCCACTAG GTGTCAATGC CAAGGGACAG CAAGTATTTC TGAAAGATAT CTGGCCGACC 
AGAGATGAGA TCCAGGCAGT GGAACGTCAG TACGTGATCC CTGGGATGTT TAAGGAGGTC 
TACCAGAAAA TAGAGACTGT GAATGAGAGC TGGAATGCCT TAGCAGCCCC GTCAGATAAG 
CTATATTGCT GGAATCCCAA ATCTACATAC ATTAAATCAC CACCATTCTT TGAAGACCTG 
ACTTTGGATC TTCAGCCCCC TAAGTCTATT GTGGATGCCT ATGTGCTATT AAACTTGGGA 
GATTCAGTAA CAACAGACCA CATCTCTCCA GCTGGAAACA TCGCAAGAAA CAGCCCTGCT 
GCTCGCTACT TAACTAACAG AGGCCTGACT CCTCGAGAAT TCAACTCCTA CGGCTCCCGC 
CGAGGGAATG ATGCCATCAT GGCACGGGGG ACATTTGCCA ACATCCGCTT ATTAAACAAA 
TTCTTGAACA AGCAGGCACC GCAGACTATC CACCTGCCTT CTGGTGAAAT TCTTGACGTG 
TTCGACGCCG CTGAGCGGTA CCAGCAGGCG GGCCTCCCCC TCATCGTCCT GGCGGGCAAA 
GAGTACGGCT CGGGCAGCTC CCGAGACTGG GCCGCCAAGG GCCCTTTCCT CCTGGGAATC 
AGAGCTGTCC TAGCAGAGAG TTACGAGCGC ATTCACCGAA GTAACTTGGT CGGGATGGGG 
GTGATCCCTC TCGAGTACCT CCCTGGGGAG AATGCAGACA CCCTGGGACT CACTGGGCGT 
GAACGATACA CTATTAGCAT TCCAGAAACC CTGAAACCAC GAATGAAAGT CCAGATCAAG 
CTGGATACCG GGAAGACCTT CCAGGCTGTC ATGAGGTTTG ACACCGACGT GGAGCTCACT 
TATTTCCATA ACGGGGGCAT CCTCAACTAC ATGATCCGCA AGATGACCAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851420.1
CDS	271..2964
Translation	MDTWSSGIMSNPFAHLVEPLDPAQPGKKFFNLNKLEDSRYGSLPFSIRVLLEAAIRNCDQFLVKKNDVENILNWKVMQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGNPEKINPICPADLVIDHSIQVDFNRRADSLKKNQDLEFERNKERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGVLGWGVGGIEAEAVMLGQPISMVLPQVIGYRLVGNPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSIKYLVQTGRDKEKVKHIKQYLQVVGMFRDFSDSSQDPDFAQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPDHHNDHKTFIYNNSKFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLSVKPYIKTSLSPGSGVVTYYLRESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEAVVEAIVQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALAAPSDKLYCWNPKSTYIKSPPFFEDLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAIMARGTFANIRLLNKFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGSGSSRDWAAKGPFLLGIRAVLAESYERIHRSNLVGMGVIPLEYLPGENADTLGLTGRERYTISIPETLKPRMKVQIKLDTGKTFQAVMRFDTDVELTYFHNGGILNYMIRKMTK

Target ORF information:

RefSeq Version	XM_024995652.1
Organism	Bos taurus(cattle)
Definition	Bos taurus aconitase 1 (ACO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995652.1

ORF Insert Sequence:

ATGGACACCT GGTCATCAGG AATCATGAGC AACCCATTTG CACACCTTGT TGAGCCTTTG 
GATCCTGCAC AACCAGGAAA GAAGTTCTTC AATTTGAATA AATTGGAGGA TTCCAGATAT 
GGATCCTTAC CATTTTCTAT CAGAGTTCTC CTGGAGGCAG CCATTCGGAA CTGTGATCAA 
TTTTTGGTGA AGAAAAATGA TGTTGAAAAT ATCCTGAATT GGAAGGTCAT GCAGCATAAG 
AACATAGAAG TGCCCTTTAA GCCTGCCCGA GTCATCCTGC AGGACTTTAC GGGTGTGCCA 
GCTGTGGTTG ACTTTGCTGC CATGCGTGAT GCTGTGAAAA AGTTAGGAGG CAATCCAGAG 
AAAATAAACC CCATCTGTCC TGCTGATCTT GTCATTGATC ATTCCATCCA GGTTGATTTC 
AACAGAAGGG CAGACAGTTT AAAGAAGAAT CAAGACTTGG AATTTGAAAG AAATAAAGAA 
CGATTTGAAT TTTTAAAGTG GGGCTCTCAG GCCTTTCACA ACATGAGGAT TATTCCACCG 
GGCTCAGGAA TCATCCACCA GGTGAATCTG GAGTATTTGG CAAGAGTGGT CTTTGATCAG 
GATGGATATT ACTACCCAGA CAGCCTTGTG GGCACAGATT CGCACACCAC CATGATTGAT 
GGTTTGGGTG TCCTTGGTTG GGGTGTGGGT GGTATCGAAG CAGAAGCTGT GATGCTGGGC 
CAGCCAATCA GCATGGTGCT TCCCCAGGTG ATCGGCTACA GGCTGGTGGG AAATCCCCAC 
CCCCTGGTCA CATCCACTGA CATCGTGCTC ACCATCACCA AGCACCTCCG CCAGGTTGGG 
GTAGTAGGCA AGTTTGTCGA GTTCTTTGGG CCAGGAGTAG CCCAACTGTC CATTGCCGAC 
CGAGCTACAA TTGCCAACAT GTGTCCAGAG TATGGGGCCA CTGCCGCCTT TTTCCCAGTT 
GATGAAGTTA GTATCAAGTA CCTGGTGCAG ACAGGTCGTG ACAAAGAAAA AGTAAAGCAT 
ATTAAACAGT ATCTTCAGGT TGTAGGAATG TTTCGGGACT TCAGTGACTC CTCTCAAGAC 
CCAGACTTTG CCCAGGTTGT GGAATTAGAC TTGAAAACAG TAGTGCCTTG CTGCAGCGGA 
CCCAAAAGGC CTCAGGACAA AGTTGCCGTG TCTGATATGA AAAAGGACTT TGAGAGCTGC 
CTGGGGGCCA AGCAAGGATT TAAAGGATTC CAGGTTGCTC CAGACCATCA TAATGACCAT 
AAGACATTTA TCTATAATAA CAGCAAGTTC ACCCTTGCTC ATGGTTCTGT GGTAATCGCT 
GCCATTACTA GCTGCACAAA CACCAGTAAT CCATCTGTGA TGTTAGGGGC AGGCTTGTTA 
GCGAAGAAGG CTGTGGATGC AGGCCTGAGC GTGAAGCCTT ACATCAAAAC TAGCCTGTCT 
CCTGGCAGTG GTGTGGTCAC CTACTACCTG CGGGAAAGCG GAGTCATGCC TTACCTGTCT 
CAGCTTGGGT TTGACGTGGT GGGCTATGGC TGCATGACCT GCATCGGCAA CAGCGGGCCC 
TTACCGGAAG CTGTGGTAGA AGCCATCGTA CAGGGGGACC TCGTAGCTGT TGGAGTACTG 
TCTGGGAACA GGAACTTTGA AGGGCGAGTC CACCCCAATA CCCGGGCCAA CTATTTAGCC 
TCTCCGCCCT TAGTAATAGC CTATGCGATT GCTGGGACCA TCAGGATCGA CTTTGAGAAA 
GAGCCACTAG GTGTCAATGC CAAGGGACAG CAAGTATTTC TGAAAGATAT CTGGCCGACC 
AGAGATGAGA TCCAGGCAGT GGAACGTCAG TACGTGATCC CTGGGATGTT TAAGGAGGTC 
TACCAGAAAA TAGAGACTGT GAATGAGAGC TGGAATGCCT TAGCAGCCCC GTCAGATAAG 
CTATATTGCT GGAATCCCAA ATCTACATAC ATTAAATCAC CACCATTCTT TGAAGACCTG 
ACTTTGGATC TTCAGCCCCC TAAGTCTATT GTGGATGCCT ATGTGCTATT AAACTTGGGA 
GATTCAGTAA CAACAGACCA CATCTCTCCA GCTGGAAACA TCGCAAGAAA CAGCCCTGCT 
GCTCGCTACT TAACTAACAG AGGCCTGACT CCTCGAGAAT TCAACTCCTA CGGCTCCCGC 
CGAGGGAATG ATGCCATCAT GGCACGGGGG ACATTTGCCA ACATCCGCTT ATTAAACAAA 
TTCTTGAACA AGCAGGCACC GCAGACTATC CACCTGCCTT CTGGTGAAAT TCTTGACGTG 
TTCGACGCCG CTGAGCGGTA CCAGCAGGCG GGCCTCCCCC TCATCGTCCT GGCGGGCAAA 
GAGTACGGCT CGGGCAGCTC CCGAGACTGG GCCGCCAAGG GCCCTTTCCT CCTGGGAATC 
AGAGCTGTCC TAGCAGAGAG TTACGAGCGC ATTCACCGAA GTAACTTGGT CGGGATGGGG 
GTGATCCCTC TCGAGTACCT CCCTGGGGAG AATGCAGACA CCCTGGGACT CACTGGGCGT 
GAACGATACA CTATTAGCAT TCCAGAAACC CTGAAACCAC GAATGAAAGT CCAGATCAAG 
CTGGATACCG GGAAGACCTT CCAGGCTGTC ATGAGGTTTG ACACCGACGT GGAGCTCACT 
TATTTCCATA ACGGGGGCAT CCTCAACTAC ATGATCCGCA AGATGACCAA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Cellular regulation of the iron-responsive element binding protein: disassembly of the cubane iron-sulfur cluster results in high-affinity RNA binding.
Proceedings of the National Academy of Sciences of the United States of America89(24)11735-9(1992 Dec)
Haile DJ,Rouault TA,Harford JB,Kennedy MC,Blondin GA,Beinert H,Klausner RD

Purification and characterization of cytosolic aconitase from beef liver and its relationship to the iron-responsive element binding protein.
Proceedings of the National Academy of Sciences of the United States of America89(24)11730-4(1992 Dec)
Kennedy MC,Mende-Mueller L,Blondin GA,Beinert H

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ACO1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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