GLI3 cDNA ORF clone, Homo sapiens(Human)

The following GLI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu26658 NM_000168.6
Latest version!
Homo sapiens GLI family zinc finger 3 (GLI3), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu26658 XM_005249704.2
Latest version!
Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu26658 XM_011515273.1
Latest version!
Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu26658 XM_011515272.1
Latest version!
Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu26658 XM_005249703.1
Latest version!
Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu57987 XM_011515274.2
Latest version!
Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 22 $811.30
$1159.00
OHu97167 XM_017011997.1
Latest version!
Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
View Old Accession Versions >>
OHu26658 NM_000168.5 Homo sapiens GLI family zinc finger 3 (GLI3), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu26658
    Clone ID Related Accession (Same CDS sequence) NM_000168.6 , XM_005249704.2 , NM_000168.5 , XM_005249703.1 , XM_011515272.1 , XM_011515273.1
    Accession Version NM_000168.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product transcriptional activator GLI3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005158.3, AI863961.1, BC113616.1, AA330409.1, DA713549.1, AC005028.2 and BI497308.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000168.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: M57609.1, BC117168.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000395925.8/ ENSP00000379258.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
    GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT
    AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG
    CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC
    GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC
    TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC
    GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG
    AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT
    GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC
    GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC
    AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC
    ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT
    GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT
    GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT
    ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC
    TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC
    ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG
    CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC
    CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA
    CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC
    CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA
    GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG
    GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG
    CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC
    AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA
    CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC
    GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC
    CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG
    CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC
    GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC
    GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC
    GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT
    GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG
    AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC
    CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG
    GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC
    AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC
    CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT
    CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG
    CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC
    CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC
    AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT
    GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG
    CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG
    CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG
    CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG
    GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC
    GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA
    GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC
    AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC
    AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT
    ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT
    GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC
    TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT
    TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA
    GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG
    GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG
    CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG
    GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000159.3
    CDS282..5024
    Translation

    Target ORF information:

    RefSeq Version NM_000168.6
    Organism Homo sapiens(human)
    Definition Homo sapiens GLI family zinc finger 3 (GLI3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000168.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
    GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT
    AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG
    CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC
    GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC
    TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC
    GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG
    AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT
    GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC
    GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC
    AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC
    ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT
    GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT
    GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT
    ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC
    TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC
    ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG
    CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC
    CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA
    CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC
    CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA
    GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG
    GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG
    CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC
    AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA
    CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC
    GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC
    CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG
    CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC
    GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC
    GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC
    GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT
    GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG
    AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC
    CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG
    GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC
    AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC
    CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT
    CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG
    CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC
    CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC
    AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT
    GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG
    CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG
    CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG
    CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG
    GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC
    GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA
    GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC
    AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC
    AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT
    ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT
    GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC
    TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT
    TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA
    GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG
    GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG
    CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG
    GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26658
    Clone ID Related Accession (Same CDS sequence) NM_000168.6 , XM_005249704.2 , NM_000168.5 , XM_005249703.1 , XM_011515272.1 , XM_011515273.1
    Accession Version NM_000168.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-10
    Organism Homo sapiens(human)
    Product transcriptional activator GLI3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI863961.1, BC113616.1, AA330409.1, DA713549.1, AC005028.2 and BI497308.1. This sequence is a reference standard in the RefSeqGene project. On Aug 7, 2008 this sequence version replaced NM_000168.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: M57609.1, BC117168.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
    GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT
    AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG
    CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC
    GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC
    TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC
    GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG
    AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT
    GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC
    GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC
    AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC
    ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT
    GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT
    GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT
    ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC
    TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC
    ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG
    CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC
    CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA
    CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC
    CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA
    GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG
    GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG
    CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC
    AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA
    CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC
    GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC
    CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG
    CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC
    GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC
    GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC
    GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT
    GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG
    AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC
    CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG
    GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC
    AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC
    CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT
    CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG
    CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC
    CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC
    AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT
    GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG
    CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG
    CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG
    CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG
    GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC
    GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA
    GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC
    AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC
    AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT
    ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT
    GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC
    TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT
    TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA
    GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG
    GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG
    CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG
    GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000159.3
    CDS92..4834
    Translation

    Target ORF information:

    RefSeq Version NM_000168.5
    Organism Homo sapiens(human)
    Definition Homo sapiens GLI family zinc finger 3 (GLI3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000168.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
    GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT
    AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG
    CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC
    GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC
    TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC
    GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG
    AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT
    GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC
    GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC
    AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC
    ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT
    GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT
    GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT
    ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC
    TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC
    ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG
    CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC
    CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA
    CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC
    CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA
    GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG
    GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG
    CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC
    AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA
    CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC
    GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC
    CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG
    CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC
    GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC
    GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC
    GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT
    GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG
    AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC
    CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG
    GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC
    AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC
    CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT
    CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG
    CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC
    CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC
    AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT
    GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG
    CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG
    CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG
    CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG
    GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC
    GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA
    GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC
    AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC
    AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT
    ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT
    GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC
    TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT
    TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA
    GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG
    GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG
    CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG
    GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26658
    Clone ID Related Accession (Same CDS sequence) NM_000168.6 , XM_005249704.2 , NM_000168.5 , XM_005249703.1 , XM_011515272.1 , XM_011515273.1
    Accession Version XM_005249704.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product transcriptional activator GLI3 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
    GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT
    AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG
    CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC
    GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC
    TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC
    GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG
    AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT
    GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC
    GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC
    AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC
    ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT
    GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT
    GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT
    ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC
    TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC
    ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG
    CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC
    CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA
    CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC
    CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA
    GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG
    GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG
    CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC
    AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA
    CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC
    GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC
    CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG
    CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC
    GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC
    GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC
    GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT
    GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG
    AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC
    CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG
    GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC
    AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC
    CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT
    CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG
    CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC
    CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC
    AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT
    GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG
    CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG
    CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG
    CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG
    GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC
    GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA
    GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC
    AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC
    AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT
    ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT
    GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC
    TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT
    TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA
    GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG
    GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG
    CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG
    GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005249761.1
    Translation

    Target ORF information:

    RefSeq Version XM_005249704.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005249704.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
    GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT
    AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG
    CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC
    GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC
    TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC
    GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG
    AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT
    GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC
    GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC
    AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC
    ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT
    GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT
    GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT
    ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC
    TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC
    ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG
    CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC
    CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA
    CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC
    CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA
    GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG
    GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG
    CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC
    AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA
    CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC
    GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC
    CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG
    CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC
    GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC
    GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC
    GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT
    GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG
    AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC
    CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG
    GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC
    AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC
    CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT
    CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG
    CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC
    CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC
    AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT
    GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG
    CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG
    CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG
    CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG
    GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC
    GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA
    GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC
    AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC
    AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT
    ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT
    GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC
    TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT
    TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA
    GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG
    GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG
    CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG
    GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26658
    Clone ID Related Accession (Same CDS sequence) NM_000168.6 , XM_005249704.2 , NM_000168.5 , XM_005249703.1 , XM_011515272.1 , XM_011515273.1
    Accession Version XM_005249703.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product transcriptional activator GLI3 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
    GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT
    AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG
    CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC
    GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC
    TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC
    GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG
    AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT
    GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC
    GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC
    AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC
    ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT
    GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT
    GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT
    ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC
    TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC
    ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG
    CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC
    CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA
    CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC
    CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA
    GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG
    GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG
    CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC
    AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA
    CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC
    GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC
    CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG
    CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC
    GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC
    GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC
    GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT
    GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG
    AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC
    CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG
    GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC
    AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC
    CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT
    CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG
    CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC
    CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC
    AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT
    GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG
    CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG
    CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG
    CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG
    GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC
    GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA
    GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC
    AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC
    AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT
    ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT
    GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC
    TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT
    TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA
    GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG
    GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG
    CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG
    GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005249760.1
    Translation

    Target ORF information:

    RefSeq Version XM_005249703.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005249703.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
    GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT
    AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG
    CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC
    GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC
    TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC
    GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG
    AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT
    GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC
    GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC
    AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC
    ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT
    GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT
    GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT
    ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC
    TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC
    ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG
    CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC
    CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA
    CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC
    CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA
    GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG
    GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG
    CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC
    AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA
    CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC
    GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC
    CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG
    CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC
    GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC
    GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC
    GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT
    GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG
    AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC
    CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG
    GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC
    AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC
    CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT
    CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG
    CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC
    CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC
    AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT
    GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG
    CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG
    CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG
    CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG
    GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC
    GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA
    GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC
    AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC
    AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT
    ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT
    GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC
    TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT
    TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA
    GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG
    GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG
    CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG
    GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26658
    Clone ID Related Accession (Same CDS sequence) NM_000168.6 , XM_005249704.2 , NM_000168.5 , XM_005249703.1 , XM_011515272.1 , XM_011515273.1
    Accession Version XM_011515272.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product transcriptional activator GLI3 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
    GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT
    AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG
    CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC
    GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC
    TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC
    GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG
    AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT
    GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC
    GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC
    AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC
    ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT
    GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT
    GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT
    ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC
    TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC
    ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG
    CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC
    CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA
    CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC
    CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA
    GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG
    GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG
    CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC
    AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA
    CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC
    GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC
    CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG
    CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC
    GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC
    GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC
    GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT
    GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG
    AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC
    CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG
    GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC
    AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC
    CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT
    CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG
    CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC
    CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC
    AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT
    GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG
    CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG
    CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG
    CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG
    GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC
    GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA
    GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC
    AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC
    AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT
    ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT
    GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC
    TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT
    TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA
    GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG
    GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG
    CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG
    GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011513574.1
    Translation

    Target ORF information:

    RefSeq Version XM_011515272.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515272.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
    GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT
    AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG
    CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC
    GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC
    TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC
    GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG
    AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT
    GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC
    GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC
    AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC
    ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT
    GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT
    GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT
    ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC
    TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC
    ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG
    CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC
    CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA
    CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC
    CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA
    GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG
    GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG
    CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC
    AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA
    CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC
    GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC
    CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG
    CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC
    GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC
    GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC
    GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT
    GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG
    AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC
    CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG
    GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC
    AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC
    CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT
    CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG
    CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC
    CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC
    AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT
    GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG
    CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG
    CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG
    CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG
    GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC
    GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA
    GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC
    AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC
    AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT
    ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT
    GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC
    TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT
    TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA
    GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG
    GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG
    CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG
    GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26658
    Clone ID Related Accession (Same CDS sequence) NM_000168.6 , XM_005249704.2 , NM_000168.5 , XM_005249703.1 , XM_011515272.1 , XM_011515273.1
    Accession Version XM_011515273.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product transcriptional activator GLI3 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
    GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT
    AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG
    CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC
    GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC
    TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC
    GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG
    AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT
    GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC
    GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC
    AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC
    ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT
    GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT
    GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT
    ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC
    TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC
    ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG
    CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC
    CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA
    CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC
    CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA
    GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG
    GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG
    CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC
    AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA
    CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC
    GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC
    CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG
    CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC
    GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC
    GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC
    GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT
    GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG
    AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC
    CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG
    GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC
    AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC
    CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT
    CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG
    CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC
    CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC
    AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT
    GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG
    CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG
    CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG
    CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG
    GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC
    GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA
    GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC
    AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC
    AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT
    ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT
    GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC
    TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT
    TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA
    GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG
    GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG
    CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG
    GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011513575.1
    Translation

    Target ORF information:

    RefSeq Version XM_011515273.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515273.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
    GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT
    AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG
    CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC
    GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC
    TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC
    CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA
    CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC
    GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG
    AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT
    GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG
    ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC
    GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC
    AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC
    GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT
    CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT
    GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT
    CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC
    ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG
    AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG
    TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT
    GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT
    GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA
    AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT
    ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC
    TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC
    ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA
    GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC
    GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG
    CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC
    CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG
    AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA
    CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC
    AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA
    ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC
    AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC
    CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC
    CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA
    GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA
    GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG
    GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG
    CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC
    AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA
    CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC
    GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC
    CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG
    CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC
    GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC
    GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC
    GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT
    GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG
    AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC
    CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG
    GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA
    AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC
    AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC
    CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT
    CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG
    CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC
    CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC
    AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT
    GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG
    CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG
    CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG
    CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG
    GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC
    GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA
    GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC
    AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC
    AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT
    ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT
    GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC
    TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT
    TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA
    GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG
    GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG
    CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG
    GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu57987
    Clone ID Related Accession (Same CDS sequence) XM_011515274.2
    Accession Version XM_011515274.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4566bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product transcriptional activator GLI3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCAGCCAC AGAATGTCCA GGGGCTCAGC AAAGTCAGTG AGGAACCTTC AACATCGAGT 
    GACGAGAGGG CCTCATTGAT CAAGAAAGAG ATCCATGGGT CCCTGCCACA CGTGGCGGAG
    CCCTCTGTGC CGTACCGCGG GACGGTGTTT GCCATGGACC CCAGGAATGG TTACATGGAG
    CCCCACTACC ACCCTCCTCA TCTTTTCCCT GCCTTCCATC CTCCTGTACC AATTGATGCC
    AGACATCATG AGGGCCGTTA CCATTACGAT CCATCTCCGA TTCCTCCATT GCATATGACT
    TCCGCCTTAT CTAGTAGCCC TACGTATCCG GACCTGCCCT TCATTAGGAT CTCCCCACAC
    CGGAACCCCA CTGCTGCTTC CGAGTCTCCC TTCAGCCCTC CACATCCCTA CATTAATCCC
    TACATGGACT ATATCCGCTC CTTGCACAGC AGCCCATCGC TCTCCATGAT CTCAGCAACC
    CGTGGGCTGA GCCCTACAGA TGCGCCCCAT GCAGGAGTCA GCCCAGCAGA ATACTATCAT
    CAGATGGCCC TGCTAACTGG CCAGCGCAGC CCCTATGCAG ACATTATTCC CTCAGCTGCC
    ACCGCCGGCA CGGGGGCCAT CCACATGGAA TATCTTCATG CTATGGATAG CACCAGATTC
    TCCAGCCCCA GGCTGTCAGC CAGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTC
    TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACGT CTCCCAACTC CTTGGTCACG
    ATTCTCAATA ATTCCCGTAG CAGCTCTTCA GCAAGTGGCT CCTATGGTCA CTTATCTGCA
    AGTGCAATCA GCCCTGCCTT GAGCTTCACC TACTCTTCCG CGCCCGTCTC TCTCCACATG
    CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGTTCAG CCTTTGGACA CAGCCCTCCA
    CTCATCCACC CTGCCCCAAC TTTTCCAACA CAGAGGCCTA TTCCAGGGAT CCCTACGGTT
    CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCTGAGTCCT CACAGAACAA GCCCACGAGT
    GAGTCTGCAG TGAGCAGCAC TGGTGACCCG ATGCACAACA AGAGGTCCAA GATCAAACCC
    GATGAAGACC TCCCCAGCCC AGGGGCTCGG GGGCAGCAGG AACAGCCCGA AGGAACAACC
    CTTGTCAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCTGAAGT CATCTATGAG
    ACAAACTGCC ACTGGGAAGG CTGCGCGAGG GAGTTCGACA CCCAAGAGCA GCTTGTGCAC
    CATATAAATA ACGACCATAT TCATGGAGAG AAGAAGGAGT TCGTGTGCAG GTGGCTGGAC
    TGCTCAAGAG AGCAGAAACC CTTCAAAGCC CAGTATATGT TGGTAGTGCA TATGAGAAGA
    CACACGGGCG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA
    CTAGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG
    CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAACA CCAAAACAGA
    ACGCATTCCA ATGAGAAACC ATATGTGTGC AAAATCCCAG GCTGCACTAA GCGTTACACA
    GACCCAAGCT CCCTCCGGAA ACATGTGAAG ACAGTGCATG GCCCAGAGGC TCATGTCACC
    AAGAAGCAGC GAGGGGACAT CCATCCTCGG CCGCCACCCC CGAGAGATTC CGGCAGCCAT
    TCACAGTCCA GGTCGCCTGG CCGACCGACT CAGGGAGCCC TTGGTGAGCA GCAGGACCTC
    AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAGGTGA AAACCGTCAA GGCAGAGAAG
    CCAATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TAGTATAGGA
    GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCAA CCATTTCCAC TGCAACCACA
    GCCCTTGCTT TGCAAGCCAG GAGAAACCCG GCAGGGACCA AATGGATGGA GCACGTAAAA
    CTAGAAAGGC TAAAACAAGT GAATGGAATG TTTCCGCGAC TGAACCCCAT TCTACCCCCT
    AAAGCCCCTG CGGTCTCTCC TCTCATAGGA AATGGCACAC AGTCCAACAA CACCTGCAGC
    TTGGGTGGGC CCATGACGCT TCTCCCGGGC AGAAGCGACC TCTCTGGGGT GGACGTCACT
    ATGCTGAACA TGCTCAACAG AAGGGACAGC AGCGCCAGCA CCATCAGCTC GGCCTACCTG
    AGCAGCCGCC GCTCCTCAGG GATCTCGCCC TGCTTCTCCA GCCGCCGCTC CAGCGAGGCG
    TCACAGGCCG AGGGCCGGCC GCAGAACGTG AGCGTGGCCG ACTCCTACGA CCCCATCTCC
    ACCGACGCCT CGCGCCGCTC CAGCGAAGCC AGCCAGAGCG ACGGCCTGCC CAGCCTGCTC
    AGCCTCACGC CCGCCCAGCA GTACCGCCTC AAGGCCAAGT ACGCGGCTGC CACAGGAGGG
    CCGCCGCCGA CGCCCCTGCC CAACATGGAG AGGATGAGCC TGAAGACGCG CCTGGCGCTG
    CTCGGGGATG CCCTCGAGCC TGGCGTGGCC CTGCCTCCAG TTCATGCCCC GAGGAGGTGC
    AGCGACGGGG GAGCCCACGG CTACGGGCGG CGCCACCTGC AGCCGCACGA TGCGCCGGGC
    CACGGCGTGA GGAGGGCCAG CGACCCGGTG CGGACAGGCT CCGAGGGCCT GGCCCTGCCT
    CGTGTGCCGC GCTTCAGCAG CCTCAGCAGC TGCAACCCCC CGGCGATGGC CACGTCCGCG
    GAGAAGCGCA GTCTCGTGCT TCAGAATTAC ACGCGGCCCG AGGGCGGCCA GTCCCGAAAC
    TTCCACTCGT CCCCCTGTCC TCCCAGCATC ACCGAGAACG TCACCCTGGA GTCCCTGACC
    ATGGACGCTG ATGCCAACCT GAACGATGAG GATTTCCTGC CGGACGACGT GGTGCAGTAT
    TTAAATTCCC AGAACCAAGC AGGGTACGAG CAGCACTTCC CCAGCGCCCT CCCGGACGAC
    AGCAAAGTGC CCCACGGGCC CGGTGACTTT GACGCGCCCG GGCTGCCAGA CAGCCACGCT
    GGCCAGCAGT TCCATGCCCT CGAGCAGCCC TGCCCCGAGG GCAGCAAAAC CGACCTGCCC
    ATTCAGTGGA ACGAAGTCAG CTCCGGAAGC GCCGACCTGT CCTCCTCCAA GCTCAAGTGT
    GGGCCGCGGC CCGCTGTGCC GCAGACTCGC GCCTTTGGGT TCTGCAACGG CATGGTCGTC
    CACCCGCAGA ACCCCTTGAG GAGCGGGCCT GCTGGGGGCT ATCAGACCCT CGGGGAGAAC
    AGCAACCCCT ACGGTGGCCC AGAGCACTTG ATGCTCCACA ACAGCCCCGG AAGTGGCACC
    AGTGGAAACG CCTTCCATGA ACAGCCCTGT AAGGCCCCGC AGTATGGGAA CTGTCTCAAC
    AGGCAGCCAG TGGCCCCTGG TGCACTCGAC GGTGCCTGTG GTGCCGGGAT TCAAGCCTCA
    AAGCTGAAGA GCACCCCCAT GCAAGGGAGC GGGGGCCAGC TGAATTTCGG CCTGCCGGTA
    GCGCCAAATG AGTCAGCTGG CAGCATGGTG AATGGCATGC AGAACCAGGA CCCAGTGGGA
    CAGGGGTACC TGGCTCACCA GCTCCTCGGC GACAGCATGC AGCACCCGGG GGCAGGCCGC
    CCCGGTCAGC AGATGCTTGG GCAGATTAGT GCTACCTCAC ACATCAACAT CTACCAAGGG
    CCAGAGAGCT GCCTGCCAGG GGCTCACGGC ATGGGCAGCC AGCCGTCAAG CTTGGCAGTT
    GTCAGGGGCT ACCAGCCATG TGCCAGCTTT GGGGGCAGCA GGCGCCAGGC TATGCCGAGG
    GACAGCCTTG CTCTGCAGTC AGGACAGCTC AGTGACACAA GTCAGACCTG CAGGGTGAAT
    GGTATCAAGA TGGAGATGAA AGGGCAGCCC CATCCGCTGT GCTCTAATCT GCAGAATTAC
    TCTGGTCAGT TCTATGACCA AACCGTGGGC TTCAGTCAGC AAGACACGAA AGCTGGTTCA
    TTCTCTATTT CAGACGCCAG CTGCCTGCTA CAGGGGACCA GCGCCAAAAA CTCTGAGTTA
    CTTTCCCCAG GTGCTAATCA GGTGACAAGC ACAGTGGACA GCCTCGACAG CCATGACCTG
    GAAGGGGTAC AGATTGACTT CGATGCCATC ATAGACGATG GGGACCACTC CAGCCTGATG
    TCGGGGGCCC TGAGCCCAAG TATCATTCAG AACCTTTCCC ATAGCTCCTC CCGCCTCACC
    ACGCCTCGGG CGTCCCTCCC ATTCCCAGCG CTGTCCATGA GCACCACCAA CATGGCTATC
    GGGGACATGA GTTCTTTGCT GACCTCCCTA GCGGAAGAAA GCAAATTCCT TGCAGTTATG
    CAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011513576.1
    CDS2893..7458
    Translation

    Target ORF information:

    RefSeq Version XM_011515274.2
    Organism Homo sapiens(human)
    Definition Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011515274.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCAGCCAC AGAATGTCCA GGGGCTCAGC AAAGTCAGTG AGGAACCTTC AACATCGAGT 
    GACGAGAGGG CCTCATTGAT CAAGAAAGAG ATCCATGGGT CCCTGCCACA CGTGGCGGAG
    CCCTCTGTGC CGTACCGCGG GACGGTGTTT GCCATGGACC CCAGGAATGG TTACATGGAG
    CCCCACTACC ACCCTCCTCA TCTTTTCCCT GCCTTCCATC CTCCTGTACC AATTGATGCC
    AGACATCATG AGGGCCGTTA CCATTACGAT CCATCTCCGA TTCCTCCATT GCATATGACT
    TCCGCCTTAT CTAGTAGCCC TACGTATCCG GACCTGCCCT TCATTAGGAT CTCCCCACAC
    CGGAACCCCA CTGCTGCTTC CGAGTCTCCC TTCAGCCCTC CACATCCCTA CATTAATCCC
    TACATGGACT ATATCCGCTC CTTGCACAGC AGCCCATCGC TCTCCATGAT CTCAGCAACC
    CGTGGGCTGA GCCCTACAGA TGCGCCCCAT GCAGGAGTCA GCCCAGCAGA ATACTATCAT
    CAGATGGCCC TGCTAACTGG CCAGCGCAGC CCCTATGCAG ACATTATTCC CTCAGCTGCC
    ACCGCCGGCA CGGGGGCCAT CCACATGGAA TATCTTCATG CTATGGATAG CACCAGATTC
    TCCAGCCCCA GGCTGTCAGC CAGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTC
    TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACGT CTCCCAACTC CTTGGTCACG
    ATTCTCAATA ATTCCCGTAG CAGCTCTTCA GCAAGTGGCT CCTATGGTCA CTTATCTGCA
    AGTGCAATCA GCCCTGCCTT GAGCTTCACC TACTCTTCCG CGCCCGTCTC TCTCCACATG
    CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGTTCAG CCTTTGGACA CAGCCCTCCA
    CTCATCCACC CTGCCCCAAC TTTTCCAACA CAGAGGCCTA TTCCAGGGAT CCCTACGGTT
    CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCTGAGTCCT CACAGAACAA GCCCACGAGT
    GAGTCTGCAG TGAGCAGCAC TGGTGACCCG ATGCACAACA AGAGGTCCAA GATCAAACCC
    GATGAAGACC TCCCCAGCCC AGGGGCTCGG GGGCAGCAGG AACAGCCCGA AGGAACAACC
    CTTGTCAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCTGAAGT CATCTATGAG
    ACAAACTGCC ACTGGGAAGG CTGCGCGAGG GAGTTCGACA CCCAAGAGCA GCTTGTGCAC
    CATATAAATA ACGACCATAT TCATGGAGAG AAGAAGGAGT TCGTGTGCAG GTGGCTGGAC
    TGCTCAAGAG AGCAGAAACC CTTCAAAGCC CAGTATATGT TGGTAGTGCA TATGAGAAGA
    CACACGGGCG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA
    CTAGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG
    CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAACA CCAAAACAGA
    ACGCATTCCA ATGAGAAACC ATATGTGTGC AAAATCCCAG GCTGCACTAA GCGTTACACA
    GACCCAAGCT CCCTCCGGAA ACATGTGAAG ACAGTGCATG GCCCAGAGGC TCATGTCACC
    AAGAAGCAGC GAGGGGACAT CCATCCTCGG CCGCCACCCC CGAGAGATTC CGGCAGCCAT
    TCACAGTCCA GGTCGCCTGG CCGACCGACT CAGGGAGCCC TTGGTGAGCA GCAGGACCTC
    AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAGGTGA AAACCGTCAA GGCAGAGAAG
    CCAATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TAGTATAGGA
    GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCAA CCATTTCCAC TGCAACCACA
    GCCCTTGCTT TGCAAGCCAG GAGAAACCCG GCAGGGACCA AATGGATGGA GCACGTAAAA
    CTAGAAAGGC TAAAACAAGT GAATGGAATG TTTCCGCGAC TGAACCCCAT TCTACCCCCT
    AAAGCCCCTG CGGTCTCTCC TCTCATAGGA AATGGCACAC AGTCCAACAA CACCTGCAGC
    TTGGGTGGGC CCATGACGCT TCTCCCGGGC AGAAGCGACC TCTCTGGGGT GGACGTCACT
    ATGCTGAACA TGCTCAACAG AAGGGACAGC AGCGCCAGCA CCATCAGCTC GGCCTACCTG
    AGCAGCCGCC GCTCCTCAGG GATCTCGCCC TGCTTCTCCA GCCGCCGCTC CAGCGAGGCG
    TCACAGGCCG AGGGCCGGCC GCAGAACGTG AGCGTGGCCG ACTCCTACGA CCCCATCTCC
    ACCGACGCCT CGCGCCGCTC CAGCGAAGCC AGCCAGAGCG ACGGCCTGCC CAGCCTGCTC
    AGCCTCACGC CCGCCCAGCA GTACCGCCTC AAGGCCAAGT ACGCGGCTGC CACAGGAGGG
    CCGCCGCCGA CGCCCCTGCC CAACATGGAG AGGATGAGCC TGAAGACGCG CCTGGCGCTG
    CTCGGGGATG CCCTCGAGCC TGGCGTGGCC CTGCCTCCAG TTCATGCCCC GAGGAGGTGC
    AGCGACGGGG GAGCCCACGG CTACGGGCGG CGCCACCTGC AGCCGCACGA TGCGCCGGGC
    CACGGCGTGA GGAGGGCCAG CGACCCGGTG CGGACAGGCT CCGAGGGCCT GGCCCTGCCT
    CGTGTGCCGC GCTTCAGCAG CCTCAGCAGC TGCAACCCCC CGGCGATGGC CACGTCCGCG
    GAGAAGCGCA GTCTCGTGCT TCAGAATTAC ACGCGGCCCG AGGGCGGCCA GTCCCGAAAC
    TTCCACTCGT CCCCCTGTCC TCCCAGCATC ACCGAGAACG TCACCCTGGA GTCCCTGACC
    ATGGACGCTG ATGCCAACCT GAACGATGAG GATTTCCTGC CGGACGACGT GGTGCAGTAT
    TTAAATTCCC AGAACCAAGC AGGGTACGAG CAGCACTTCC CCAGCGCCCT CCCGGACGAC
    AGCAAAGTGC CCCACGGGCC CGGTGACTTT GACGCGCCCG GGCTGCCAGA CAGCCACGCT
    GGCCAGCAGT TCCATGCCCT CGAGCAGCCC TGCCCCGAGG GCAGCAAAAC CGACCTGCCC
    ATTCAGTGGA ACGAAGTCAG CTCCGGAAGC GCCGACCTGT CCTCCTCCAA GCTCAAGTGT
    GGGCCGCGGC CCGCTGTGCC GCAGACTCGC GCCTTTGGGT TCTGCAACGG CATGGTCGTC
    CACCCGCAGA ACCCCTTGAG GAGCGGGCCT GCTGGGGGCT ATCAGACCCT CGGGGAGAAC
    AGCAACCCCT ACGGTGGCCC AGAGCACTTG ATGCTCCACA ACAGCCCCGG AAGTGGCACC
    AGTGGAAACG CCTTCCATGA ACAGCCCTGT AAGGCCCCGC AGTATGGGAA CTGTCTCAAC
    AGGCAGCCAG TGGCCCCTGG TGCACTCGAC GGTGCCTGTG GTGCCGGGAT TCAAGCCTCA
    AAGCTGAAGA GCACCCCCAT GCAAGGGAGC GGGGGCCAGC TGAATTTCGG CCTGCCGGTA
    GCGCCAAATG AGTCAGCTGG CAGCATGGTG AATGGCATGC AGAACCAGGA CCCAGTGGGA
    CAGGGGTACC TGGCTCACCA GCTCCTCGGC GACAGCATGC AGCACCCGGG GGCAGGCCGC
    CCCGGTCAGC AGATGCTTGG GCAGATTAGT GCTACCTCAC ACATCAACAT CTACCAAGGG
    CCAGAGAGCT GCCTGCCAGG GGCTCACGGC ATGGGCAGCC AGCCGTCAAG CTTGGCAGTT
    GTCAGGGGCT ACCAGCCATG TGCCAGCTTT GGGGGCAGCA GGCGCCAGGC TATGCCGAGG
    GACAGCCTTG CTCTGCAGTC AGGACAGCTC AGTGACACAA GTCAGACCTG CAGGGTGAAT
    GGTATCAAGA TGGAGATGAA AGGGCAGCCC CATCCGCTGT GCTCTAATCT GCAGAATTAC
    TCTGGTCAGT TCTATGACCA AACCGTGGGC TTCAGTCAGC AAGACACGAA AGCTGGTTCA
    TTCTCTATTT CAGACGCCAG CTGCCTGCTA CAGGGGACCA GCGCCAAAAA CTCTGAGTTA
    CTTTCCCCAG GTGCTAATCA GGTGACAAGC ACAGTGGACA GCCTCGACAG CCATGACCTG
    GAAGGGGTAC AGATTGACTT CGATGCCATC ATAGACGATG GGGACCACTC CAGCCTGATG
    TCGGGGGCCC TGAGCCCAAG TATCATTCAG AACCTTTCCC ATAGCTCCTC CCGCCTCACC
    ACGCCTCGGG CGTCCCTCCC ATTCCCAGCG CTGTCCATGA GCACCACCAA CATGGCTATC
    GGGGACATGA GTTCTTTGCT GACCTCCCTA GCGGAAGAAA GCAAATTCCT TGCAGTTATG
    CAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu97167
    Clone ID Related Accession (Same CDS sequence) XM_017011997.1
    Accession Version XM_017011997.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product transcriptional activator GLI3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCATCCGC GCTGTCCATC CCTGCTGCTG CACAAACGAC TCAGCCACAG ATCCTCTTGG 
    ATGGGCTACC TTGAAAGGGG GAAGCTCCAA AGAATGCGTA CAAACCCATA CATTTACCTA
    GAGGATGAAA GTCCTGGACA GACTTATCAC AGAGAGAGAA GAAACGCAAT CACTATGCAG
    CCACAGAATG TCCAGGGGCT CAGCAAAGTC AGTGAGGAAC CTTCAACATC GAGTGACGAG
    AGGGCCTCAT TGATCAAGAA AGAGATCCAT GGGTCCCTGC CACACGTGGC GGAGCCCTCT
    GTGCCGTACC GCGGGACGGT GTTTGCCATG GACCCCAGGA ATGGTTACAT GGAGCCCCAC
    TACCACCCTC CTCATCTTTT CCCTGCCTTC CATCCTCCTG TACCAATTGA TGCCAGACAT
    CATGAGGGCC GTTACCATTA CGATCCATCT CCGATTCCTC CATTGCATAT GACTTCCGCC
    TTATCTAGTA GCCCTACGTA TCCGGACCTG CCCTTCATTA GGATCTCCCC ACACCGGAAC
    CCCACTGCTG CTTCCGAGTC TCCCTTCAGC CCTCCACATC CCTACATTAA TCCCTACATG
    GACTATATCC GCTCCTTGCA CAGCAGCCCA TCGCTCTCCA TGATCTCAGC AACCCGTGGG
    CTGAGCCCTA CAGATGCGCC CCATGCAGGA GTCAGCCCAG CAGAATACTA TCATCAGATG
    GCCCTGCTAA CTGGCCAGCG CAGCCCCTAT GCAGACATTA TTCCCTCAGC TGCCACCGCC
    GGCACGGGGG CCATCCACAT GGAATATCTT CATGCTATGG ATAGCACCAG ATTCTCCAGC
    CCCAGGCTGT CAGCCAGGCC GAGCCGAAAA CGTACACTGT CCATATCACC ACTCTCCGAT
    CATAGCTTTG ACCTTCAGAC CATGATAAGG ACGTCTCCCA ACTCCTTGGT CACGATTCTC
    AATAATTCCC GTAGCAGCTC TTCAGCAAGT GGCTCCTATG GTCACTTATC TGCAAGTGCA
    ATCAGCCCTG CCTTGAGCTT CACCTACTCT TCCGCGCCCG TCTCTCTCCA CATGCATCAG
    CAGATCCTAA GCCGACAACA GAGCTTAGGT TCAGCCTTTG GACACAGCCC TCCACTCATC
    CACCCTGCCC CAACTTTTCC AACACAGAGG CCTATTCCAG GGATCCCTAC GGTTCTGAAC
    CCCGTCCAGG TCAGCTCCGG CCCTTCTGAG TCCTCACAGA ACAAGCCCAC GAGTGAGTCT
    GCAGTGAGCA GCACTGGTGA CCCGATGCAC AACAAGAGGT CCAAGATCAA ACCCGATGAA
    GACCTCCCCA GCCCAGGGGC TCGGGGGCAG CAGGAACAGC CCGAAGGAAC AACCCTTGTC
    AAGGAGGAAG GGGACAAAGA TGAAAGCAAA CAGGAGCCTG AAGTCATCTA TGAGACAAAC
    TGCCACTGGG AAGGCTGCGC GAGGGAGTTC GACACCCAAG AGCAGCTTGT GCACCATATA
    AATAACGACC ATATTCATGG AGAGAAGAAG GAGTTCGTGT GCAGGTGGCT GGACTGCTCA
    AGAGAGCAGA AACCCTTCAA AGCCCAGTAT ATGTTGGTAG TGCATATGAG AAGACACACG
    GGCGAGAAGC CTCACAAATG CACTTTTGAA GGTTGCACAA AGGCCTACTC GAGACTAGAA
    AACTTGAAAA CACACTTGAG ATCTCACACT GGAGAGAAAC CATACGTCTG TGAGCACGAA
    GGTTGCAACA AGGCTTTCTC AAATGCCTCT GATCGCGCCA AACACCAAAA CAGAACGCAT
    TCCAATGAGA AACCATATGT GTGCAAAATC CCAGGCTGCA CTAAGCGTTA CACAGACCCA
    AGCTCCCTCC GGAAACATGT GAAGACAGTG CATGGCCCAG AGGCTCATGT CACCAAGAAG
    CAGCGAGGGG ACATCCATCC TCGGCCGCCA CCCCCGAGAG ATTCCGGCAG CCATTCACAG
    TCCAGGTCGC CTGGCCGACC GACTCAGGGA GCCCTTGGTG AGCAGCAGGA CCTCAGCAAC
    ACTACCTCAA AGCGGGAAGA ATGCCTCCAG GTGAAAACCG TCAAGGCAGA GAAGCCAATG
    ACATCTCAGC CAAGCCCTGG TGGTCAGTCT TCATGCAGCA GCCAACAGTC CCCCATCAGC
    AACTATTCCA ACAGTGGGCT CGAGCTTCCT CTGACCGATG GAGGTAGTAT AGGAGACCTC
    AGTGCCATCG ATGAAACCCC AATCATGGAC TCAACCATTT CCACTGCAAC CACAGCCCTT
    GCTTTGCAAG CCAGGAGAAA CCCGGCAGGG ACCAAATGGA TGGAGCACGT AAAACTAGAA
    AGGCTAAAAC AAGTGAATGG AATGTTTCCG CGACTGAACC CCATTCTACC CCCTAAAGCC
    CCTGCGGTCT CTCCTCTCAT AGGAAATGGC ACACAGTCCA ACAACACCTG CAGCTTGGGT
    GGGCCCATGA CGCTTCTCCC GGGCAGAAGC GACCTCTCTG GGGTGGACGT CACTATGCTG
    AACATGCTCA ACAGAAGGGA CAGCAGCGCC AGCACCATCA GCTCGGCCTA CCTGAGCAGC
    CGCCGCTCCT CAGGGATCTC GCCCTGCTTC TCCAGCCGCC GCTCCAGCGA GGCGTCACAG
    GCCGAGGGCC GGCCGCAGAA CGTGAGCGTG GCCGACTCCT ACGACCCCAT CTCCACCGAC
    GCCTCGCGCC GCTCCAGCGA AGCCAGCCAG AGCGACGGCC TGCCCAGCCT GCTCAGCCTC
    ACGCCCGCCC AGCAGTACCG CCTCAAGGCC AAGTACGCGG CTGCCACAGG AGGGCCGCCG
    CCGACGCCCC TGCCCAACAT GGAGAGGATG AGCCTGAAGA CGCGCCTGGC GCTGCTCGGG
    GATGCCCTCG AGCCTGGCGT GGCCCTGCCT CCAGTTCATG CCCCGAGGAG GTGCAGCGAC
    GGGGGAGCCC ACGGCTACGG GCGGCGCCAC CTGCAGCCGC ACGATGCGCC GGGCCACGGC
    GTGAGGAGGG CCAGCGACCC GGTGCGGACA GGCTCCGAGG GCCTGGCCCT GCCTCGTGTG
    CCGCGCTTCA GCAGCCTCAG CAGCTGCAAC CCCCCGGCGA TGGCCACGTC CGCGGAGAAG
    CGCAGTCTCG TGCTTCAGAA TTACACGCGG CCCGAGGGCG GCCAGTCCCG AAACTTCCAC
    TCGTCCCCCT GTCCTCCCAG CATCACCGAG AACGTCACCC TGGAGTCCCT GACCATGGAC
    GCTGATGCCA ACCTGAACGA TGAGGATTTC CTGCCGGACG ACGTGGTGCA GTATTTAAAT
    TCCCAGAACC AAGCAGGGTA CGAGCAGCAC TTCCCCAGCG CCCTCCCGGA CGACAGCAAA
    GTGCCCCACG GGCCCGGTGA CTTTGACGCG CCCGGGCTGC CAGACAGCCA CGCTGGCCAG
    CAGTTCCATG CCCTCGAGCA GCCCTGCCCC GAGGGCAGCA AAACCGACCT GCCCATTCAG
    TGGAACGAAG TCAGCTCCGG AAGCGCCGAC CTGTCCTCCT CCAAGCTCAA GTGTGGGCCG
    CGGCCCGCTG TGCCGCAGAC TCGCGCCTTT GGGTTCTGCA ACGGCATGGT CGTCCACCCG
    CAGAACCCCT TGAGGAGCGG GCCTGCTGGG GGCTATCAGA CCCTCGGGGA GAACAGCAAC
    CCCTACGGTG GCCCAGAGCA CTTGATGCTC CACAACAGCC CCGGAAGTGG CACCAGTGGA
    AACGCCTTCC ATGAACAGCC CTGTAAGGCC CCGCAGTATG GGAACTGTCT CAACAGGCAG
    CCAGTGGCCC CTGGTGCACT CGACGGTGCC TGTGGTGCCG GGATTCAAGC CTCAAAGCTG
    AAGAGCACCC CCATGCAAGG GAGCGGGGGC CAGCTGAATT TCGGCCTGCC GGTAGCGCCA
    AATGAGTCAG CTGGCAGCAT GGTGAATGGC ATGCAGAACC AGGACCCAGT GGGACAGGGG
    TACCTGGCTC ACCAGCTCCT CGGCGACAGC ATGCAGCACC CGGGGGCAGG CCGCCCCGGT
    CAGCAGATGC TTGGGCAGAT TAGTGCTACC TCACACATCA ACATCTACCA AGGGCCAGAG
    AGCTGCCTGC CAGGGGCTCA CGGCATGGGC AGCCAGCCGT CAAGCTTGGC AGTTGTCAGG
    GGCTACCAGC CATGTGCCAG CTTTGGGGGC AGCAGGCGCC AGGCTATGCC GAGGGACAGC
    CTTGCTCTGC AGTCAGGACA GCTCAGTGAC ACAAGTCAGA CCTGCAGGGT GAATGGTATC
    AAGATGGAGA TGAAAGGGCA GCCCCATCCG CTGTGCTCTA ATCTGCAGAA TTACTCTGGT
    CAGTTCTATG ACCAAACCGT GGGCTTCAGT CAGCAAGACA CGAAAGCTGG TTCATTCTCT
    ATTTCAGACG CCAGCTGCCT GCTACAGGGG ACCAGCGCCA AAAACTCTGA GTTACTTTCC
    CCAGGTGCTA ATCAGGTGAC AAGCACAGTG GACAGCCTCG ACAGCCATGA CCTGGAAGGG
    GTACAGATTG ACTTCGATGC CATCATAGAC GATGGGGACC ACTCCAGCCT GATGTCGGGG
    GCCCTGAGCC CAAGTATCAT TCAGAACCTT TCCCATAGCT CCTCCCGCCT CACCACGCCT
    CGGGCGTCCC TCCCATTCCC AGCGCTGTCC ATGAGCACCA CCAACATGGC TATCGGGGAC
    ATGAGTTCTT TGCTGACCTC CCTAGCGGAA GAAAGCAAAT TCCTTGCAGT TATGCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016867486.1
    CDS2752..7491
    Translation

    Target ORF information:

    RefSeq Version XM_017011997.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017011997.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCATCCGC GCTGTCCATC CCTGCTGCTG CACAAACGAC TCAGCCACAG ATCCTCTTGG 
    ATGGGCTACC TTGAAAGGGG GAAGCTCCAA AGAATGCGTA CAAACCCATA CATTTACCTA
    GAGGATGAAA GTCCTGGACA GACTTATCAC AGAGAGAGAA GAAACGCAAT CACTATGCAG
    CCACAGAATG TCCAGGGGCT CAGCAAAGTC AGTGAGGAAC CTTCAACATC GAGTGACGAG
    AGGGCCTCAT TGATCAAGAA AGAGATCCAT GGGTCCCTGC CACACGTGGC GGAGCCCTCT
    GTGCCGTACC GCGGGACGGT GTTTGCCATG GACCCCAGGA ATGGTTACAT GGAGCCCCAC
    TACCACCCTC CTCATCTTTT CCCTGCCTTC CATCCTCCTG TACCAATTGA TGCCAGACAT
    CATGAGGGCC GTTACCATTA CGATCCATCT CCGATTCCTC CATTGCATAT GACTTCCGCC
    TTATCTAGTA GCCCTACGTA TCCGGACCTG CCCTTCATTA GGATCTCCCC ACACCGGAAC
    CCCACTGCTG CTTCCGAGTC TCCCTTCAGC CCTCCACATC CCTACATTAA TCCCTACATG
    GACTATATCC GCTCCTTGCA CAGCAGCCCA TCGCTCTCCA TGATCTCAGC AACCCGTGGG
    CTGAGCCCTA CAGATGCGCC CCATGCAGGA GTCAGCCCAG CAGAATACTA TCATCAGATG
    GCCCTGCTAA CTGGCCAGCG CAGCCCCTAT GCAGACATTA TTCCCTCAGC TGCCACCGCC
    GGCACGGGGG CCATCCACAT GGAATATCTT CATGCTATGG ATAGCACCAG ATTCTCCAGC
    CCCAGGCTGT CAGCCAGGCC GAGCCGAAAA CGTACACTGT CCATATCACC ACTCTCCGAT
    CATAGCTTTG ACCTTCAGAC CATGATAAGG ACGTCTCCCA ACTCCTTGGT CACGATTCTC
    AATAATTCCC GTAGCAGCTC TTCAGCAAGT GGCTCCTATG GTCACTTATC TGCAAGTGCA
    ATCAGCCCTG CCTTGAGCTT CACCTACTCT TCCGCGCCCG TCTCTCTCCA CATGCATCAG
    CAGATCCTAA GCCGACAACA GAGCTTAGGT TCAGCCTTTG GACACAGCCC TCCACTCATC
    CACCCTGCCC CAACTTTTCC AACACAGAGG CCTATTCCAG GGATCCCTAC GGTTCTGAAC
    CCCGTCCAGG TCAGCTCCGG CCCTTCTGAG TCCTCACAGA ACAAGCCCAC GAGTGAGTCT
    GCAGTGAGCA GCACTGGTGA CCCGATGCAC AACAAGAGGT CCAAGATCAA ACCCGATGAA
    GACCTCCCCA GCCCAGGGGC TCGGGGGCAG CAGGAACAGC CCGAAGGAAC AACCCTTGTC
    AAGGAGGAAG GGGACAAAGA TGAAAGCAAA CAGGAGCCTG AAGTCATCTA TGAGACAAAC
    TGCCACTGGG AAGGCTGCGC GAGGGAGTTC GACACCCAAG AGCAGCTTGT GCACCATATA
    AATAACGACC ATATTCATGG AGAGAAGAAG GAGTTCGTGT GCAGGTGGCT GGACTGCTCA
    AGAGAGCAGA AACCCTTCAA AGCCCAGTAT ATGTTGGTAG TGCATATGAG AAGACACACG
    GGCGAGAAGC CTCACAAATG CACTTTTGAA GGTTGCACAA AGGCCTACTC GAGACTAGAA
    AACTTGAAAA CACACTTGAG ATCTCACACT GGAGAGAAAC CATACGTCTG TGAGCACGAA
    GGTTGCAACA AGGCTTTCTC AAATGCCTCT GATCGCGCCA AACACCAAAA CAGAACGCAT
    TCCAATGAGA AACCATATGT GTGCAAAATC CCAGGCTGCA CTAAGCGTTA CACAGACCCA
    AGCTCCCTCC GGAAACATGT GAAGACAGTG CATGGCCCAG AGGCTCATGT CACCAAGAAG
    CAGCGAGGGG ACATCCATCC TCGGCCGCCA CCCCCGAGAG ATTCCGGCAG CCATTCACAG
    TCCAGGTCGC CTGGCCGACC GACTCAGGGA GCCCTTGGTG AGCAGCAGGA CCTCAGCAAC
    ACTACCTCAA AGCGGGAAGA ATGCCTCCAG GTGAAAACCG TCAAGGCAGA GAAGCCAATG
    ACATCTCAGC CAAGCCCTGG TGGTCAGTCT TCATGCAGCA GCCAACAGTC CCCCATCAGC
    AACTATTCCA ACAGTGGGCT CGAGCTTCCT CTGACCGATG GAGGTAGTAT AGGAGACCTC
    AGTGCCATCG ATGAAACCCC AATCATGGAC TCAACCATTT CCACTGCAAC CACAGCCCTT
    GCTTTGCAAG CCAGGAGAAA CCCGGCAGGG ACCAAATGGA TGGAGCACGT AAAACTAGAA
    AGGCTAAAAC AAGTGAATGG AATGTTTCCG CGACTGAACC CCATTCTACC CCCTAAAGCC
    CCTGCGGTCT CTCCTCTCAT AGGAAATGGC ACACAGTCCA ACAACACCTG CAGCTTGGGT
    GGGCCCATGA CGCTTCTCCC GGGCAGAAGC GACCTCTCTG GGGTGGACGT CACTATGCTG
    AACATGCTCA ACAGAAGGGA CAGCAGCGCC AGCACCATCA GCTCGGCCTA CCTGAGCAGC
    CGCCGCTCCT CAGGGATCTC GCCCTGCTTC TCCAGCCGCC GCTCCAGCGA GGCGTCACAG
    GCCGAGGGCC GGCCGCAGAA CGTGAGCGTG GCCGACTCCT ACGACCCCAT CTCCACCGAC
    GCCTCGCGCC GCTCCAGCGA AGCCAGCCAG AGCGACGGCC TGCCCAGCCT GCTCAGCCTC
    ACGCCCGCCC AGCAGTACCG CCTCAAGGCC AAGTACGCGG CTGCCACAGG AGGGCCGCCG
    CCGACGCCCC TGCCCAACAT GGAGAGGATG AGCCTGAAGA CGCGCCTGGC GCTGCTCGGG
    GATGCCCTCG AGCCTGGCGT GGCCCTGCCT CCAGTTCATG CCCCGAGGAG GTGCAGCGAC
    GGGGGAGCCC ACGGCTACGG GCGGCGCCAC CTGCAGCCGC ACGATGCGCC GGGCCACGGC
    GTGAGGAGGG CCAGCGACCC GGTGCGGACA GGCTCCGAGG GCCTGGCCCT GCCTCGTGTG
    CCGCGCTTCA GCAGCCTCAG CAGCTGCAAC CCCCCGGCGA TGGCCACGTC CGCGGAGAAG
    CGCAGTCTCG TGCTTCAGAA TTACACGCGG CCCGAGGGCG GCCAGTCCCG AAACTTCCAC
    TCGTCCCCCT GTCCTCCCAG CATCACCGAG AACGTCACCC TGGAGTCCCT GACCATGGAC
    GCTGATGCCA ACCTGAACGA TGAGGATTTC CTGCCGGACG ACGTGGTGCA GTATTTAAAT
    TCCCAGAACC AAGCAGGGTA CGAGCAGCAC TTCCCCAGCG CCCTCCCGGA CGACAGCAAA
    GTGCCCCACG GGCCCGGTGA CTTTGACGCG CCCGGGCTGC CAGACAGCCA CGCTGGCCAG
    CAGTTCCATG CCCTCGAGCA GCCCTGCCCC GAGGGCAGCA AAACCGACCT GCCCATTCAG
    TGGAACGAAG TCAGCTCCGG AAGCGCCGAC CTGTCCTCCT CCAAGCTCAA GTGTGGGCCG
    CGGCCCGCTG TGCCGCAGAC TCGCGCCTTT GGGTTCTGCA ACGGCATGGT CGTCCACCCG
    CAGAACCCCT TGAGGAGCGG GCCTGCTGGG GGCTATCAGA CCCTCGGGGA GAACAGCAAC
    CCCTACGGTG GCCCAGAGCA CTTGATGCTC CACAACAGCC CCGGAAGTGG CACCAGTGGA
    AACGCCTTCC ATGAACAGCC CTGTAAGGCC CCGCAGTATG GGAACTGTCT CAACAGGCAG
    CCAGTGGCCC CTGGTGCACT CGACGGTGCC TGTGGTGCCG GGATTCAAGC CTCAAAGCTG
    AAGAGCACCC CCATGCAAGG GAGCGGGGGC CAGCTGAATT TCGGCCTGCC GGTAGCGCCA
    AATGAGTCAG CTGGCAGCAT GGTGAATGGC ATGCAGAACC AGGACCCAGT GGGACAGGGG
    TACCTGGCTC ACCAGCTCCT CGGCGACAGC ATGCAGCACC CGGGGGCAGG CCGCCCCGGT
    CAGCAGATGC TTGGGCAGAT TAGTGCTACC TCACACATCA ACATCTACCA AGGGCCAGAG
    AGCTGCCTGC CAGGGGCTCA CGGCATGGGC AGCCAGCCGT CAAGCTTGGC AGTTGTCAGG
    GGCTACCAGC CATGTGCCAG CTTTGGGGGC AGCAGGCGCC AGGCTATGCC GAGGGACAGC
    CTTGCTCTGC AGTCAGGACA GCTCAGTGAC ACAAGTCAGA CCTGCAGGGT GAATGGTATC
    AAGATGGAGA TGAAAGGGCA GCCCCATCCG CTGTGCTCTA ATCTGCAGAA TTACTCTGGT
    CAGTTCTATG ACCAAACCGT GGGCTTCAGT CAGCAAGACA CGAAAGCTGG TTCATTCTCT
    ATTTCAGACG CCAGCTGCCT GCTACAGGGG ACCAGCGCCA AAAACTCTGA GTTACTTTCC
    CCAGGTGCTA ATCAGGTGAC AAGCACAGTG GACAGCCTCG ACAGCCATGA CCTGGAAGGG
    GTACAGATTG ACTTCGATGC CATCATAGAC GATGGGGACC ACTCCAGCCT GATGTCGGGG
    GCCCTGAGCC CAAGTATCAT TCAGAACCTT TCCCATAGCT CCTCCCGCCT CACCACGCCT
    CGGGCGTCCC TCCCATTCCC AGCGCTGTCC ATGAGCACCA CCAACATGGC TATCGGGGAC
    ATGAGTTCTT TGCTGACCTC CCTAGCGGAA GAAAGCAAAT TCCTTGCAGT TATGCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.