GLI3 cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GLI3
Entrez Gene ID	2737
Full Name	GLI family zinc finger 3
Synonyms	ACLS,GCPS,GLI3-190,GLI3FL,PAP-A,PAPA,PAPA1,PAPB,PHS,PPDIV
General protein information	Preferred Names GLI family zinc finger 3 Names transcriptional activator GLI3 GLI-Kruppel family member GLI3 glioma-associated oncogene family zinc finger 3 oncogene GLI3 zinc finger protein GLI3
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 7 Map Location: 7p14.1
Summary	This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008].
Disorder MIM:	165240
Disorder Html:	Greig cephalopolysyndactyly syndrome, 175700 (3); Pallister-Hall syndrome, 146510 (3); Polydactyly, preaxial, type IV, 174700 (3); Polydactyly, postaxial, types A1 and B, 174200 (3); {Hypothalamic hamartomas, somatic}, 241800 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000168.6	NP_000159.3	transcriptional activator GLI3
XM_005249704.2	XP_005249761.1	transcriptional activator GLI3 isoform X1
XM_017011997.1	XP_016867486.1	transcriptional activator GLI3 isoform X1
NM_000168.5	NP_000159.3	transcriptional activator GLI3
XM_005249703.1	XP_005249760.1	transcriptional activator GLI3 isoform X1
XM_011515272.1	XP_011513574.1	transcriptional activator GLI3 isoform X1
XM_011515274.2	XP_011513576.1	transcriptional activator GLI3 isoform X2
XM_011515273.1	XP_011513575.1	transcriptional activator GLI3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GLI3	NP_000159.3
Rattus norvegicus (Norway rat)	Gli3	NP_536330.1
Danio rerio (zebrafish)	gli3	NP_991291.1
Pan troglodytes (chimpanzee)	GLI3	NP_001029362.1
Canis lupus familiaris (dog)	GLI3	XP_005630984.1
Bos taurus (cattle)	LOC785371	XP_005205717.1
Gallus gallus (chicken)	GLI3	NP_001258832.1
	GLI3	XP_001098108.1
Mus musculus (house mouse)	Gli3	NP_032156.2
Xenopus tropicalis (tropical clawed frog)	gli3	XP_004915408.1

Gene Ontology
Bibliography

Related articles in PubMed

[A novel mutation of GLI3 gene underlying synpolydactyly in a family].
Kang R, Huang S, Li J, Zou Y, Xu P, Gao M, Wang L, Xie H, Yan J, Gao Y
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics34(4)490-493(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The c.480dupC of the GLI3 gene probably underlies the synpolydactyly in this family.
Title: [A novel mutation of GLI3 gene underlying synpolydactyly in a family].

Data suggest that negative feedback mediated by GLI3 (GLI-Kruppel family member) acts to finely tune SHH (sonic hedgehog) signaling. During medulloblastoma (MB) formation, nerve tissue cells appear to express nestin which hyperactivates SHH signaling by abolishing negative feedback by GLI3. Restoration of intrinsic negative feedback by repressing nestin expression represents a promising approach to treat MB. [REVIEW]
Title: Restore the brake on tumor progression.

the first report of the assessment of the frequency of GLI3/SHH/preZRS/ZRS in Chinese polydactyly patients to show any higher possibility of mutations or variants for the 4 genes or sequences in China
Title: Mutational screening of GLI3, SHH, preZRS, and ZRS in 102 Chinese children with nonsyndromic polydactyly.

a novel GLI3 mutation c.714T>A (p.Y238*) was identified in a Chinese family with pre-axial polydactyly. Our results broadened the phenotypic spectrum of GLI3 mutations and demonstrated the feasibility of WES in clinical application of molecular diagnosis.
Title: Exome sequencing reveals a novel nonsense mutation of GLI3 in a Chinese family with 'non-syndromic' pre-axial polydactyly.

Gli3 and Teashirt3 might play an important role in the normal development of the ureter.
Title: The Expression of Gli3 and Teashirt3 in the Stenotic Tissue of Congenital Pelvi-Ureteric Junction Obstruction in Children.

The following GLI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26658	NM_000168.6 Latest version!	Homo sapiens GLI family zinc finger 3 (GLI3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu26658	XM_005249704.2 Latest version!	Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu26658	XM_011515273.1 Latest version!	Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu26658	XM_011515272.1 Latest version!	Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu26658	XM_005249703.1 Latest version!	Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu57987	XM_011515274.2 Latest version!	Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$811.30 $1159.00
OHu97167	XM_017011997.1 Latest version!	Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OHu26658	NM_000168.5	Homo sapiens GLI family zinc finger 3 (GLI3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GLI3 ( NM_000168.6 ) cDNA ORF clone, Homo sapiens(Human) -> NP_000159.3 Homo sapiens GLI family zinc finger 3 (GLI3), mRNA.

Price & Availability↑

CloneID	OHu26658
Clone ID Related Accession (Same CDS sequence)	NM_000168.6 , XM_005249704.2 , NM_000168.5 , XM_005249703.1 , XM_011515272.1 , XM_011515273.1
Accession Version	NM_000168.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	transcriptional activator GLI3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005158.3, AI863961.1, BC113616.1, AA330409.1, DA713549.1, AC005028.2 and BI497308.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000168.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: M57609.1, BC117168.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000395925.8/ ENSP00000379258.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000159.3
CDS	282..5024
Translation	MEAQSHSSTTTEKKKVENSIVKCSTRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQNVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHMTSALSSSPTYPDLPFIRISPHRNPTAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISATRGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADIIPSAATAGTGAIHMEYLHAMDSTRFSSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYSSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGARGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCAREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRPTQGALGEQQDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSIGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMFPRLNPILPPKAPAVSPLIGNGTQSNNTCSLGGPMTLLPGRSDLSGVDVTMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQSDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRLALLGDALEPGVALPPVHAPRRCSDGGAHGYGRRHLQPHDAPGHGVRRASDPVRTGSEGLALPRVPRFSSLSSCNPPAMATSAEKRSLVLQNYTRPEGGQSRNFHSSPCPPSITENVTLESLTMDADANLNDEDFLPDDVVQYLNSQNQAGYEQHFPSALPDDSKVPHGPGDFDAPGLPDSHAGQQFHALEQPCPEGSKTDLPIQWNEVSSGSADLSSSKLKCGPRPAVPQTRAFGFCNGMVVHPQNPLRSGPAGGYQTLGENSNPYGGPEHLMLHNSPGSGTSGNAFHEQPCKAPQYGNCLNRQPVAPGALDGACGAGIQASKLKSTPMQGSGGQLNFGLPVAPNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAGRPGQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHSSLMSGALSPSIIQNLSHSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	NM_000168.6
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 3 (GLI3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000168.6

ORF Insert Sequence:

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( NM_000168.5 ) cDNA ORF clone, Homo sapiens(Human) -> NP_000159.3 Homo sapiens GLI family zinc finger 3 (GLI3), mRNA.

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CloneID	OHu26658
Clone ID Related Accession (Same CDS sequence)	NM_000168.6 , XM_005249704.2 , NM_000168.5 , XM_005249703.1 , XM_011515272.1 , XM_011515273.1
Accession Version	NM_000168.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	transcriptional activator GLI3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI863961.1, BC113616.1, AA330409.1, DA713549.1, AC005028.2 and BI497308.1. This sequence is a reference standard in the RefSeqGene project. On Aug 7, 2008 this sequence version replaced NM_000168.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: M57609.1, BC117168.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000159.3
CDS	92..4834
Translation	MEAQSHSSTTTEKKKVENSIVKCSTRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQNVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHMTSALSSSPTYPDLPFIRISPHRNPTAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISATRGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADIIPSAATAGTGAIHMEYLHAMDSTRFSSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYSSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGARGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCAREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRPTQGALGEQQDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSIGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMFPRLNPILPPKAPAVSPLIGNGTQSNNTCSLGGPMTLLPGRSDLSGVDVTMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQSDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRLALLGDALEPGVALPPVHAPRRCSDGGAHGYGRRHLQPHDAPGHGVRRASDPVRTGSEGLALPRVPRFSSLSSCNPPAMATSAEKRSLVLQNYTRPEGGQSRNFHSSPCPPSITENVTLESLTMDADANLNDEDFLPDDVVQYLNSQNQAGYEQHFPSALPDDSKVPHGPGDFDAPGLPDSHAGQQFHALEQPCPEGSKTDLPIQWNEVSSGSADLSSSKLKCGPRPAVPQTRAFGFCNGMVVHPQNPLRSGPAGGYQTLGENSNPYGGPEHLMLHNSPGSGTSGNAFHEQPCKAPQYGNCLNRQPVAPGALDGACGAGIQASKLKSTPMQGSGGQLNFGLPVAPNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAGRPGQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHSSLMSGALSPSIIQNLSHSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	NM_000168.5
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 3 (GLI3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000168.5

ORF Insert Sequence:

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_005249704.2 ) cDNA ORF clone, Homo sapiens(Human) -> XP_005249761.1 Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu26658
Clone ID Related Accession (Same CDS sequence)	NM_000168.6 , XM_005249704.2 , NM_000168.5 , XM_005249703.1 , XM_011515272.1 , XM_011515273.1
Accession Version	XM_005249704.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	transcriptional activator GLI3 isoform X1
Comment

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005249761.1

Translation

MEAQSHSSTTTEKKKVENSIVKCSTRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQNVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHMTSALSSSPTYPDLPFIRISPHRNPTAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISATRGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADIIPSAATAGTGAIHMEYLHAMDSTRFSSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYSSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGARGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCAREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRPTQGALGEQQDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSIGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMFPRLNPILPPKAPAVSPLIGNGTQSNNTCSLGGPMTLLPGRSDLSGVDVTMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQSDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRLALLGDALEPGVALPPVHAPRRCSDGGAHGYGRRHLQPHDAPGHGVRRASDPVRTGSEGLALPRVPRFSSLSSCNPPAMATSAEKRSLVLQNYTRPEGGQSRNFHSSPCPPSITENVTLESLTMDADANLNDEDFLPDDVVQYLNSQNQAGYEQHFPSALPDDSKVPHGPGDFDAPGLPDSHAGQQFHALEQPCPEGSKTDLPIQWNEVSSGSADLSSSKLKCGPRPAVPQTRAFGFCNGMVVHPQNPLRSGPAGGYQTLGENSNPYGGPEHLMLHNSPGSGTSGNAFHEQPCKAPQYGNCLNRQPVAPGALDGACGAGIQASKLKSTPMQGSGGQLNFGLPVAPNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAGRPGQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHSSLMSGALSPSIIQNLSHSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_005249704.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249704.2

ORF Insert Sequence:

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_005249703.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_005249760.1 Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

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CloneID	OHu26658
Clone ID Related Accession (Same CDS sequence)	NM_000168.6 , XM_005249704.2 , NM_000168.5 , XM_005249703.1 , XM_011515272.1 , XM_011515273.1
Accession Version	XM_005249703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	transcriptional activator GLI3 isoform X1
Comment

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005249760.1

Translation

MEAQSHSSTTTEKKKVENSIVKCSTRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQNVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHMTSALSSSPTYPDLPFIRISPHRNPTAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISATRGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADIIPSAATAGTGAIHMEYLHAMDSTRFSSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYSSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGARGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCAREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRPTQGALGEQQDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSIGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMFPRLNPILPPKAPAVSPLIGNGTQSNNTCSLGGPMTLLPGRSDLSGVDVTMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQSDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRLALLGDALEPGVALPPVHAPRRCSDGGAHGYGRRHLQPHDAPGHGVRRASDPVRTGSEGLALPRVPRFSSLSSCNPPAMATSAEKRSLVLQNYTRPEGGQSRNFHSSPCPPSITENVTLESLTMDADANLNDEDFLPDDVVQYLNSQNQAGYEQHFPSALPDDSKVPHGPGDFDAPGLPDSHAGQQFHALEQPCPEGSKTDLPIQWNEVSSGSADLSSSKLKCGPRPAVPQTRAFGFCNGMVVHPQNPLRSGPAGGYQTLGENSNPYGGPEHLMLHNSPGSGTSGNAFHEQPCKAPQYGNCLNRQPVAPGALDGACGAGIQASKLKSTPMQGSGGQLNFGLPVAPNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAGRPGQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHSSLMSGALSPSIIQNLSHSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_005249703.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249703.1

ORF Insert Sequence:

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_011515272.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011513574.1 Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.

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CloneID	OHu26658
Clone ID Related Accession (Same CDS sequence)	NM_000168.6 , XM_005249704.2 , NM_000168.5 , XM_005249703.1 , XM_011515272.1 , XM_011515273.1
Accession Version	XM_011515272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	transcriptional activator GLI3 isoform X1
Comment

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011513574.1

Translation

MEAQSHSSTTTEKKKVENSIVKCSTRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQNVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHMTSALSSSPTYPDLPFIRISPHRNPTAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISATRGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADIIPSAATAGTGAIHMEYLHAMDSTRFSSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYSSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGARGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCAREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRPTQGALGEQQDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSIGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMFPRLNPILPPKAPAVSPLIGNGTQSNNTCSLGGPMTLLPGRSDLSGVDVTMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQSDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRLALLGDALEPGVALPPVHAPRRCSDGGAHGYGRRHLQPHDAPGHGVRRASDPVRTGSEGLALPRVPRFSSLSSCNPPAMATSAEKRSLVLQNYTRPEGGQSRNFHSSPCPPSITENVTLESLTMDADANLNDEDFLPDDVVQYLNSQNQAGYEQHFPSALPDDSKVPHGPGDFDAPGLPDSHAGQQFHALEQPCPEGSKTDLPIQWNEVSSGSADLSSSKLKCGPRPAVPQTRAFGFCNGMVVHPQNPLRSGPAGGYQTLGENSNPYGGPEHLMLHNSPGSGTSGNAFHEQPCKAPQYGNCLNRQPVAPGALDGACGAGIQASKLKSTPMQGSGGQLNFGLPVAPNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAGRPGQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHSSLMSGALSPSIIQNLSHSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_011515272.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515272.1

ORF Insert Sequence:

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_011515273.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011513575.1 Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu26658
Clone ID Related Accession (Same CDS sequence)	NM_000168.6 , XM_005249704.2 , NM_000168.5 , XM_005249703.1 , XM_011515272.1 , XM_011515273.1
Accession Version	XM_011515273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	transcriptional activator GLI3 isoform X1
Comment

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011513575.1

Translation

MEAQSHSSTTTEKKKVENSIVKCSTRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQNVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHMTSALSSSPTYPDLPFIRISPHRNPTAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISATRGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADIIPSAATAGTGAIHMEYLHAMDSTRFSSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYSSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGARGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCAREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRPTQGALGEQQDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSIGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMFPRLNPILPPKAPAVSPLIGNGTQSNNTCSLGGPMTLLPGRSDLSGVDVTMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQSDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRLALLGDALEPGVALPPVHAPRRCSDGGAHGYGRRHLQPHDAPGHGVRRASDPVRTGSEGLALPRVPRFSSLSSCNPPAMATSAEKRSLVLQNYTRPEGGQSRNFHSSPCPPSITENVTLESLTMDADANLNDEDFLPDDVVQYLNSQNQAGYEQHFPSALPDDSKVPHGPGDFDAPGLPDSHAGQQFHALEQPCPEGSKTDLPIQWNEVSSGSADLSSSKLKCGPRPAVPQTRAFGFCNGMVVHPQNPLRSGPAGGYQTLGENSNPYGGPEHLMLHNSPGSGTSGNAFHEQPCKAPQYGNCLNRQPVAPGALDGACGAGIQASKLKSTPMQGSGGQLNFGLPVAPNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAGRPGQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHSSLMSGALSPSIIQNLSHSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_011515273.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515273.1

ORF Insert Sequence:

ATGGAGGCCC AGTCCCACAG CTCCACGACC ACTGAAAAGA AAAAAGTTGA GAATTCCATA 
GTGAAGTGCT CCACTCGAAC AGATGTGAGC GAGAAAGCCG TTGCCTCCAG CACCACTTCT 
AATGAGGATG AAAGTCCTGG ACAGACTTAT CACAGAGAGA GAAGAAACGC AATCACTATG 
CAGCCACAGA ATGTCCAGGG GCTCAGCAAA GTCAGTGAGG AACCTTCAAC ATCGAGTGAC 
GAGAGGGCCT CATTGATCAA GAAAGAGATC CATGGGTCCC TGCCACACGT GGCGGAGCCC 
TCTGTGCCGT ACCGCGGGAC GGTGTTTGCC ATGGACCCCA GGAATGGTTA CATGGAGCCC 
CACTACCACC CTCCTCATCT TTTCCCTGCC TTCCATCCTC CTGTACCAAT TGATGCCAGA 
CATCATGAGG GCCGTTACCA TTACGATCCA TCTCCGATTC CTCCATTGCA TATGACTTCC 
GCCTTATCTA GTAGCCCTAC GTATCCGGAC CTGCCCTTCA TTAGGATCTC CCCACACCGG 
AACCCCACTG CTGCTTCCGA GTCTCCCTTC AGCCCTCCAC ATCCCTACAT TAATCCCTAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCGCTCT CCATGATCTC AGCAACCCGT 
GGGCTGAGCC CTACAGATGC GCCCCATGCA GGAGTCAGCC CAGCAGAATA CTATCATCAG 
ATGGCCCTGC TAACTGGCCA GCGCAGCCCC TATGCAGACA TTATTCCCTC AGCTGCCACC 
GCCGGCACGG GGGCCATCCA CATGGAATAT CTTCATGCTA TGGATAGCAC CAGATTCTCC 
AGCCCCAGGC TGTCAGCCAG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCACTCTCC 
GATCATAGCT TTGACCTTCA GACCATGATA AGGACGTCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGTAGCAG CTCTTCAGCA AGTGGCTCCT ATGGTCACTT ATCTGCAAGT 
GCAATCAGCC CTGCCTTGAG CTTCACCTAC TCTTCCGCGC CCGTCTCTCT CCACATGCAT 
CAGCAGATCC TAAGCCGACA ACAGAGCTTA GGTTCAGCCT TTGGACACAG CCCTCCACTC 
ATCCACCCTG CCCCAACTTT TCCAACACAG AGGCCTATTC CAGGGATCCC TACGGTTCTG 
AACCCCGTCC AGGTCAGCTC CGGCCCTTCT GAGTCCTCAC AGAACAAGCC CACGAGTGAG 
TCTGCAGTGA GCAGCACTGG TGACCCGATG CACAACAAGA GGTCCAAGAT CAAACCCGAT 
GAAGACCTCC CCAGCCCAGG GGCTCGGGGG CAGCAGGAAC AGCCCGAAGG AACAACCCTT 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAACAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CGCGAGGGAG TTCGACACCC AAGAGCAGCT TGTGCACCAT 
ATAAATAACG ACCATATTCA TGGAGAGAAG AAGGAGTTCG TGTGCAGGTG GCTGGACTGC 
TCAAGAGAGC AGAAACCCTT CAAAGCCCAG TATATGTTGG TAGTGCATAT GAGAAGACAC 
ACGGGCGAGA AGCCTCACAA ATGCACTTTT GAAGGTTGCA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACACACTT GAGATCTCAC ACTGGAGAGA AACCATACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAACACCA AAACAGAACG 
CATTCCAATG AGAAACCATA TGTGTGCAAA ATCCCAGGCT GCACTAAGCG TTACACAGAC 
CCAAGCTCCC TCCGGAAACA TGTGAAGACA GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGAG GGGACATCCA TCCTCGGCCG CCACCCCCGA GAGATTCCGG CAGCCATTCA 
CAGTCCAGGT CGCCTGGCCG ACCGACTCAG GGAGCCCTTG GTGAGCAGCA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAAA CCGTCAAGGC AGAGAAGCCA 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACCG ATGGAGGTAG TATAGGAGAC 
CTCAGTGCCA TCGATGAAAC CCCAATCATG GACTCAACCA TTTCCACTGC AACCACAGCC 
CTTGCTTTGC AAGCCAGGAG AAACCCGGCA GGGACCAAAT GGATGGAGCA CGTAAAACTA 
GAAAGGCTAA AACAAGTGAA TGGAATGTTT CCGCGACTGA ACCCCATTCT ACCCCCTAAA 
GCCCCTGCGG TCTCTCCTCT CATAGGAAAT GGCACACAGT CCAACAACAC CTGCAGCTTG 
GGTGGGCCCA TGACGCTTCT CCCGGGCAGA AGCGACCTCT CTGGGGTGGA CGTCACTATG 
CTGAACATGC TCAACAGAAG GGACAGCAGC GCCAGCACCA TCAGCTCGGC CTACCTGAGC 
AGCCGCCGCT CCTCAGGGAT CTCGCCCTGC TTCTCCAGCC GCCGCTCCAG CGAGGCGTCA 
CAGGCCGAGG GCCGGCCGCA GAACGTGAGC GTGGCCGACT CCTACGACCC CATCTCCACC 
GACGCCTCGC GCCGCTCCAG CGAAGCCAGC CAGAGCGACG GCCTGCCCAG CCTGCTCAGC 
CTCACGCCCG CCCAGCAGTA CCGCCTCAAG GCCAAGTACG CGGCTGCCAC AGGAGGGCCG 
CCGCCGACGC CCCTGCCCAA CATGGAGAGG ATGAGCCTGA AGACGCGCCT GGCGCTGCTC 
GGGGATGCCC TCGAGCCTGG CGTGGCCCTG CCTCCAGTTC ATGCCCCGAG GAGGTGCAGC 
GACGGGGGAG CCCACGGCTA CGGGCGGCGC CACCTGCAGC CGCACGATGC GCCGGGCCAC 
GGCGTGAGGA GGGCCAGCGA CCCGGTGCGG ACAGGCTCCG AGGGCCTGGC CCTGCCTCGT 
GTGCCGCGCT TCAGCAGCCT CAGCAGCTGC AACCCCCCGG CGATGGCCAC GTCCGCGGAG 
AAGCGCAGTC TCGTGCTTCA GAATTACACG CGGCCCGAGG GCGGCCAGTC CCGAAACTTC 
CACTCGTCCC CCTGTCCTCC CAGCATCACC GAGAACGTCA CCCTGGAGTC CCTGACCATG 
GACGCTGATG CCAACCTGAA CGATGAGGAT TTCCTGCCGG ACGACGTGGT GCAGTATTTA 
AATTCCCAGA ACCAAGCAGG GTACGAGCAG CACTTCCCCA GCGCCCTCCC GGACGACAGC 
AAAGTGCCCC ACGGGCCCGG TGACTTTGAC GCGCCCGGGC TGCCAGACAG CCACGCTGGC 
CAGCAGTTCC ATGCCCTCGA GCAGCCCTGC CCCGAGGGCA GCAAAACCGA CCTGCCCATT 
CAGTGGAACG AAGTCAGCTC CGGAAGCGCC GACCTGTCCT CCTCCAAGCT CAAGTGTGGG 
CCGCGGCCCG CTGTGCCGCA GACTCGCGCC TTTGGGTTCT GCAACGGCAT GGTCGTCCAC 
CCGCAGAACC CCTTGAGGAG CGGGCCTGCT GGGGGCTATC AGACCCTCGG GGAGAACAGC 
AACCCCTACG GTGGCCCAGA GCACTTGATG CTCCACAACA GCCCCGGAAG TGGCACCAGT 
GGAAACGCCT TCCATGAACA GCCCTGTAAG GCCCCGCAGT ATGGGAACTG TCTCAACAGG 
CAGCCAGTGG CCCCTGGTGC ACTCGACGGT GCCTGTGGTG CCGGGATTCA AGCCTCAAAG 
CTGAAGAGCA CCCCCATGCA AGGGAGCGGG GGCCAGCTGA ATTTCGGCCT GCCGGTAGCG 
CCAAATGAGT CAGCTGGCAG CATGGTGAAT GGCATGCAGA ACCAGGACCC AGTGGGACAG 
GGGTACCTGG CTCACCAGCT CCTCGGCGAC AGCATGCAGC ACCCGGGGGC AGGCCGCCCC 
GGTCAGCAGA TGCTTGGGCA GATTAGTGCT ACCTCACACA TCAACATCTA CCAAGGGCCA 
GAGAGCTGCC TGCCAGGGGC TCACGGCATG GGCAGCCAGC CGTCAAGCTT GGCAGTTGTC 
AGGGGCTACC AGCCATGTGC CAGCTTTGGG GGCAGCAGGC GCCAGGCTAT GCCGAGGGAC 
AGCCTTGCTC TGCAGTCAGG ACAGCTCAGT GACACAAGTC AGACCTGCAG GGTGAATGGT 
ATCAAGATGG AGATGAAAGG GCAGCCCCAT CCGCTGTGCT CTAATCTGCA GAATTACTCT 
GGTCAGTTCT ATGACCAAAC CGTGGGCTTC AGTCAGCAAG ACACGAAAGC TGGTTCATTC 
TCTATTTCAG ACGCCAGCTG CCTGCTACAG GGGACCAGCG CCAAAAACTC TGAGTTACTT 
TCCCCAGGTG CTAATCAGGT GACAAGCACA GTGGACAGCC TCGACAGCCA TGACCTGGAA 
GGGGTACAGA TTGACTTCGA TGCCATCATA GACGATGGGG ACCACTCCAG CCTGATGTCG 
GGGGCCCTGA GCCCAAGTAT CATTCAGAAC CTTTCCCATA GCTCCTCCCG CCTCACCACG 
CCTCGGGCGT CCCTCCCATT CCCAGCGCTG TCCATGAGCA CCACCAACAT GGCTATCGGG 
GACATGAGTT CTTTGCTGAC CTCCCTAGCG GAAGAAAGCA AATTCCTTGC AGTTATGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_011515274.2 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011513576.1 Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu57987
Clone ID Related Accession (Same CDS sequence)	XM_011515274.2
Accession Version	XM_011515274.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcriptional activator GLI3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCAC AGAATGTCCA GGGGCTCAGC AAAGTCAGTG AGGAACCTTC AACATCGAGT 
GACGAGAGGG CCTCATTGAT CAAGAAAGAG ATCCATGGGT CCCTGCCACA CGTGGCGGAG 
CCCTCTGTGC CGTACCGCGG GACGGTGTTT GCCATGGACC CCAGGAATGG TTACATGGAG 
CCCCACTACC ACCCTCCTCA TCTTTTCCCT GCCTTCCATC CTCCTGTACC AATTGATGCC 
AGACATCATG AGGGCCGTTA CCATTACGAT CCATCTCCGA TTCCTCCATT GCATATGACT 
TCCGCCTTAT CTAGTAGCCC TACGTATCCG GACCTGCCCT TCATTAGGAT CTCCCCACAC 
CGGAACCCCA CTGCTGCTTC CGAGTCTCCC TTCAGCCCTC CACATCCCTA CATTAATCCC 
TACATGGACT ATATCCGCTC CTTGCACAGC AGCCCATCGC TCTCCATGAT CTCAGCAACC 
CGTGGGCTGA GCCCTACAGA TGCGCCCCAT GCAGGAGTCA GCCCAGCAGA ATACTATCAT 
CAGATGGCCC TGCTAACTGG CCAGCGCAGC CCCTATGCAG ACATTATTCC CTCAGCTGCC 
ACCGCCGGCA CGGGGGCCAT CCACATGGAA TATCTTCATG CTATGGATAG CACCAGATTC 
TCCAGCCCCA GGCTGTCAGC CAGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTC 
TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACGT CTCCCAACTC CTTGGTCACG 
ATTCTCAATA ATTCCCGTAG CAGCTCTTCA GCAAGTGGCT CCTATGGTCA CTTATCTGCA 
AGTGCAATCA GCCCTGCCTT GAGCTTCACC TACTCTTCCG CGCCCGTCTC TCTCCACATG 
CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGTTCAG CCTTTGGACA CAGCCCTCCA 
CTCATCCACC CTGCCCCAAC TTTTCCAACA CAGAGGCCTA TTCCAGGGAT CCCTACGGTT 
CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCTGAGTCCT CACAGAACAA GCCCACGAGT 
GAGTCTGCAG TGAGCAGCAC TGGTGACCCG ATGCACAACA AGAGGTCCAA GATCAAACCC 
GATGAAGACC TCCCCAGCCC AGGGGCTCGG GGGCAGCAGG AACAGCCCGA AGGAACAACC 
CTTGTCAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCTGAAGT CATCTATGAG 
ACAAACTGCC ACTGGGAAGG CTGCGCGAGG GAGTTCGACA CCCAAGAGCA GCTTGTGCAC 
CATATAAATA ACGACCATAT TCATGGAGAG AAGAAGGAGT TCGTGTGCAG GTGGCTGGAC 
TGCTCAAGAG AGCAGAAACC CTTCAAAGCC CAGTATATGT TGGTAGTGCA TATGAGAAGA 
CACACGGGCG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA 
CTAGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG 
CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAACA CCAAAACAGA 
ACGCATTCCA ATGAGAAACC ATATGTGTGC AAAATCCCAG GCTGCACTAA GCGTTACACA 
GACCCAAGCT CCCTCCGGAA ACATGTGAAG ACAGTGCATG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GAGGGGACAT CCATCCTCGG CCGCCACCCC CGAGAGATTC CGGCAGCCAT 
TCACAGTCCA GGTCGCCTGG CCGACCGACT CAGGGAGCCC TTGGTGAGCA GCAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAGGTGA AAACCGTCAA GGCAGAGAAG 
CCAATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TAGTATAGGA 
GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCAA CCATTTCCAC TGCAACCACA 
GCCCTTGCTT TGCAAGCCAG GAGAAACCCG GCAGGGACCA AATGGATGGA GCACGTAAAA 
CTAGAAAGGC TAAAACAAGT GAATGGAATG TTTCCGCGAC TGAACCCCAT TCTACCCCCT 
AAAGCCCCTG CGGTCTCTCC TCTCATAGGA AATGGCACAC AGTCCAACAA CACCTGCAGC 
TTGGGTGGGC CCATGACGCT TCTCCCGGGC AGAAGCGACC TCTCTGGGGT GGACGTCACT 
ATGCTGAACA TGCTCAACAG AAGGGACAGC AGCGCCAGCA CCATCAGCTC GGCCTACCTG 
AGCAGCCGCC GCTCCTCAGG GATCTCGCCC TGCTTCTCCA GCCGCCGCTC CAGCGAGGCG 
TCACAGGCCG AGGGCCGGCC GCAGAACGTG AGCGTGGCCG ACTCCTACGA CCCCATCTCC 
ACCGACGCCT CGCGCCGCTC CAGCGAAGCC AGCCAGAGCG ACGGCCTGCC CAGCCTGCTC 
AGCCTCACGC CCGCCCAGCA GTACCGCCTC AAGGCCAAGT ACGCGGCTGC CACAGGAGGG 
CCGCCGCCGA CGCCCCTGCC CAACATGGAG AGGATGAGCC TGAAGACGCG CCTGGCGCTG 
CTCGGGGATG CCCTCGAGCC TGGCGTGGCC CTGCCTCCAG TTCATGCCCC GAGGAGGTGC 
AGCGACGGGG GAGCCCACGG CTACGGGCGG CGCCACCTGC AGCCGCACGA TGCGCCGGGC 
CACGGCGTGA GGAGGGCCAG CGACCCGGTG CGGACAGGCT CCGAGGGCCT GGCCCTGCCT 
CGTGTGCCGC GCTTCAGCAG CCTCAGCAGC TGCAACCCCC CGGCGATGGC CACGTCCGCG 
GAGAAGCGCA GTCTCGTGCT TCAGAATTAC ACGCGGCCCG AGGGCGGCCA GTCCCGAAAC 
TTCCACTCGT CCCCCTGTCC TCCCAGCATC ACCGAGAACG TCACCCTGGA GTCCCTGACC 
ATGGACGCTG ATGCCAACCT GAACGATGAG GATTTCCTGC CGGACGACGT GGTGCAGTAT 
TTAAATTCCC AGAACCAAGC AGGGTACGAG CAGCACTTCC CCAGCGCCCT CCCGGACGAC 
AGCAAAGTGC CCCACGGGCC CGGTGACTTT GACGCGCCCG GGCTGCCAGA CAGCCACGCT 
GGCCAGCAGT TCCATGCCCT CGAGCAGCCC TGCCCCGAGG GCAGCAAAAC CGACCTGCCC 
ATTCAGTGGA ACGAAGTCAG CTCCGGAAGC GCCGACCTGT CCTCCTCCAA GCTCAAGTGT 
GGGCCGCGGC CCGCTGTGCC GCAGACTCGC GCCTTTGGGT TCTGCAACGG CATGGTCGTC 
CACCCGCAGA ACCCCTTGAG GAGCGGGCCT GCTGGGGGCT ATCAGACCCT CGGGGAGAAC 
AGCAACCCCT ACGGTGGCCC AGAGCACTTG ATGCTCCACA ACAGCCCCGG AAGTGGCACC 
AGTGGAAACG CCTTCCATGA ACAGCCCTGT AAGGCCCCGC AGTATGGGAA CTGTCTCAAC 
AGGCAGCCAG TGGCCCCTGG TGCACTCGAC GGTGCCTGTG GTGCCGGGAT TCAAGCCTCA 
AAGCTGAAGA GCACCCCCAT GCAAGGGAGC GGGGGCCAGC TGAATTTCGG CCTGCCGGTA 
GCGCCAAATG AGTCAGCTGG CAGCATGGTG AATGGCATGC AGAACCAGGA CCCAGTGGGA 
CAGGGGTACC TGGCTCACCA GCTCCTCGGC GACAGCATGC AGCACCCGGG GGCAGGCCGC 
CCCGGTCAGC AGATGCTTGG GCAGATTAGT GCTACCTCAC ACATCAACAT CTACCAAGGG 
CCAGAGAGCT GCCTGCCAGG GGCTCACGGC ATGGGCAGCC AGCCGTCAAG CTTGGCAGTT 
GTCAGGGGCT ACCAGCCATG TGCCAGCTTT GGGGGCAGCA GGCGCCAGGC TATGCCGAGG 
GACAGCCTTG CTCTGCAGTC AGGACAGCTC AGTGACACAA GTCAGACCTG CAGGGTGAAT 
GGTATCAAGA TGGAGATGAA AGGGCAGCCC CATCCGCTGT GCTCTAATCT GCAGAATTAC 
TCTGGTCAGT TCTATGACCA AACCGTGGGC TTCAGTCAGC AAGACACGAA AGCTGGTTCA 
TTCTCTATTT CAGACGCCAG CTGCCTGCTA CAGGGGACCA GCGCCAAAAA CTCTGAGTTA 
CTTTCCCCAG GTGCTAATCA GGTGACAAGC ACAGTGGACA GCCTCGACAG CCATGACCTG 
GAAGGGGTAC AGATTGACTT CGATGCCATC ATAGACGATG GGGACCACTC CAGCCTGATG 
TCGGGGGCCC TGAGCCCAAG TATCATTCAG AACCTTTCCC ATAGCTCCTC CCGCCTCACC 
ACGCCTCGGG CGTCCCTCCC ATTCCCAGCG CTGTCCATGA GCACCACCAA CATGGCTATC 
GGGGACATGA GTTCTTTGCT GACCTCCCTA GCGGAAGAAA GCAAATTCCT TGCAGTTATG 
CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513576.1
CDS	2893..7458
Translation	MQPQNVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHMTSALSSSPTYPDLPFIRISPHRNPTAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISATRGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADIIPSAATAGTGAIHMEYLHAMDSTRFSSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYSSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGARGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCAREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRPTQGALGEQQDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSIGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMFPRLNPILPPKAPAVSPLIGNGTQSNNTCSLGGPMTLLPGRSDLSGVDVTMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQSDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRLALLGDALEPGVALPPVHAPRRCSDGGAHGYGRRHLQPHDAPGHGVRRASDPVRTGSEGLALPRVPRFSSLSSCNPPAMATSAEKRSLVLQNYTRPEGGQSRNFHSSPCPPSITENVTLESLTMDADANLNDEDFLPDDVVQYLNSQNQAGYEQHFPSALPDDSKVPHGPGDFDAPGLPDSHAGQQFHALEQPCPEGSKTDLPIQWNEVSSGSADLSSSKLKCGPRPAVPQTRAFGFCNGMVVHPQNPLRSGPAGGYQTLGENSNPYGGPEHLMLHNSPGSGTSGNAFHEQPCKAPQYGNCLNRQPVAPGALDGACGAGIQASKLKSTPMQGSGGQLNFGLPVAPNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAGRPGQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHSSLMSGALSPSIIQNLSHSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_011515274.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515274.2

ORF Insert Sequence:

ATGCAGCCAC AGAATGTCCA GGGGCTCAGC AAAGTCAGTG AGGAACCTTC AACATCGAGT 
GACGAGAGGG CCTCATTGAT CAAGAAAGAG ATCCATGGGT CCCTGCCACA CGTGGCGGAG 
CCCTCTGTGC CGTACCGCGG GACGGTGTTT GCCATGGACC CCAGGAATGG TTACATGGAG 
CCCCACTACC ACCCTCCTCA TCTTTTCCCT GCCTTCCATC CTCCTGTACC AATTGATGCC 
AGACATCATG AGGGCCGTTA CCATTACGAT CCATCTCCGA TTCCTCCATT GCATATGACT 
TCCGCCTTAT CTAGTAGCCC TACGTATCCG GACCTGCCCT TCATTAGGAT CTCCCCACAC 
CGGAACCCCA CTGCTGCTTC CGAGTCTCCC TTCAGCCCTC CACATCCCTA CATTAATCCC 
TACATGGACT ATATCCGCTC CTTGCACAGC AGCCCATCGC TCTCCATGAT CTCAGCAACC 
CGTGGGCTGA GCCCTACAGA TGCGCCCCAT GCAGGAGTCA GCCCAGCAGA ATACTATCAT 
CAGATGGCCC TGCTAACTGG CCAGCGCAGC CCCTATGCAG ACATTATTCC CTCAGCTGCC 
ACCGCCGGCA CGGGGGCCAT CCACATGGAA TATCTTCATG CTATGGATAG CACCAGATTC 
TCCAGCCCCA GGCTGTCAGC CAGGCCGAGC CGAAAACGTA CACTGTCCAT ATCACCACTC 
TCCGATCATA GCTTTGACCT TCAGACCATG ATAAGGACGT CTCCCAACTC CTTGGTCACG 
ATTCTCAATA ATTCCCGTAG CAGCTCTTCA GCAAGTGGCT CCTATGGTCA CTTATCTGCA 
AGTGCAATCA GCCCTGCCTT GAGCTTCACC TACTCTTCCG CGCCCGTCTC TCTCCACATG 
CATCAGCAGA TCCTAAGCCG ACAACAGAGC TTAGGTTCAG CCTTTGGACA CAGCCCTCCA 
CTCATCCACC CTGCCCCAAC TTTTCCAACA CAGAGGCCTA TTCCAGGGAT CCCTACGGTT 
CTGAACCCCG TCCAGGTCAG CTCCGGCCCT TCTGAGTCCT CACAGAACAA GCCCACGAGT 
GAGTCTGCAG TGAGCAGCAC TGGTGACCCG ATGCACAACA AGAGGTCCAA GATCAAACCC 
GATGAAGACC TCCCCAGCCC AGGGGCTCGG GGGCAGCAGG AACAGCCCGA AGGAACAACC 
CTTGTCAAGG AGGAAGGGGA CAAAGATGAA AGCAAACAGG AGCCTGAAGT CATCTATGAG 
ACAAACTGCC ACTGGGAAGG CTGCGCGAGG GAGTTCGACA CCCAAGAGCA GCTTGTGCAC 
CATATAAATA ACGACCATAT TCATGGAGAG AAGAAGGAGT TCGTGTGCAG GTGGCTGGAC 
TGCTCAAGAG AGCAGAAACC CTTCAAAGCC CAGTATATGT TGGTAGTGCA TATGAGAAGA 
CACACGGGCG AGAAGCCTCA CAAATGCACT TTTGAAGGTT GCACAAAGGC CTACTCGAGA 
CTAGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA CGTCTGTGAG 
CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAACA CCAAAACAGA 
ACGCATTCCA ATGAGAAACC ATATGTGTGC AAAATCCCAG GCTGCACTAA GCGTTACACA 
GACCCAAGCT CCCTCCGGAA ACATGTGAAG ACAGTGCATG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GAGGGGACAT CCATCCTCGG CCGCCACCCC CGAGAGATTC CGGCAGCCAT 
TCACAGTCCA GGTCGCCTGG CCGACCGACT CAGGGAGCCC TTGGTGAGCA GCAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAGGTGA AAACCGTCAA GGCAGAGAAG 
CCAATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGATGGAGG TAGTATAGGA 
GACCTCAGTG CCATCGATGA AACCCCAATC ATGGACTCAA CCATTTCCAC TGCAACCACA 
GCCCTTGCTT TGCAAGCCAG GAGAAACCCG GCAGGGACCA AATGGATGGA GCACGTAAAA 
CTAGAAAGGC TAAAACAAGT GAATGGAATG TTTCCGCGAC TGAACCCCAT TCTACCCCCT 
AAAGCCCCTG CGGTCTCTCC TCTCATAGGA AATGGCACAC AGTCCAACAA CACCTGCAGC 
TTGGGTGGGC CCATGACGCT TCTCCCGGGC AGAAGCGACC TCTCTGGGGT GGACGTCACT 
ATGCTGAACA TGCTCAACAG AAGGGACAGC AGCGCCAGCA CCATCAGCTC GGCCTACCTG 
AGCAGCCGCC GCTCCTCAGG GATCTCGCCC TGCTTCTCCA GCCGCCGCTC CAGCGAGGCG 
TCACAGGCCG AGGGCCGGCC GCAGAACGTG AGCGTGGCCG ACTCCTACGA CCCCATCTCC 
ACCGACGCCT CGCGCCGCTC CAGCGAAGCC AGCCAGAGCG ACGGCCTGCC CAGCCTGCTC 
AGCCTCACGC CCGCCCAGCA GTACCGCCTC AAGGCCAAGT ACGCGGCTGC CACAGGAGGG 
CCGCCGCCGA CGCCCCTGCC CAACATGGAG AGGATGAGCC TGAAGACGCG CCTGGCGCTG 
CTCGGGGATG CCCTCGAGCC TGGCGTGGCC CTGCCTCCAG TTCATGCCCC GAGGAGGTGC 
AGCGACGGGG GAGCCCACGG CTACGGGCGG CGCCACCTGC AGCCGCACGA TGCGCCGGGC 
CACGGCGTGA GGAGGGCCAG CGACCCGGTG CGGACAGGCT CCGAGGGCCT GGCCCTGCCT 
CGTGTGCCGC GCTTCAGCAG CCTCAGCAGC TGCAACCCCC CGGCGATGGC CACGTCCGCG 
GAGAAGCGCA GTCTCGTGCT TCAGAATTAC ACGCGGCCCG AGGGCGGCCA GTCCCGAAAC 
TTCCACTCGT CCCCCTGTCC TCCCAGCATC ACCGAGAACG TCACCCTGGA GTCCCTGACC 
ATGGACGCTG ATGCCAACCT GAACGATGAG GATTTCCTGC CGGACGACGT GGTGCAGTAT 
TTAAATTCCC AGAACCAAGC AGGGTACGAG CAGCACTTCC CCAGCGCCCT CCCGGACGAC 
AGCAAAGTGC CCCACGGGCC CGGTGACTTT GACGCGCCCG GGCTGCCAGA CAGCCACGCT 
GGCCAGCAGT TCCATGCCCT CGAGCAGCCC TGCCCCGAGG GCAGCAAAAC CGACCTGCCC 
ATTCAGTGGA ACGAAGTCAG CTCCGGAAGC GCCGACCTGT CCTCCTCCAA GCTCAAGTGT 
GGGCCGCGGC CCGCTGTGCC GCAGACTCGC GCCTTTGGGT TCTGCAACGG CATGGTCGTC 
CACCCGCAGA ACCCCTTGAG GAGCGGGCCT GCTGGGGGCT ATCAGACCCT CGGGGAGAAC 
AGCAACCCCT ACGGTGGCCC AGAGCACTTG ATGCTCCACA ACAGCCCCGG AAGTGGCACC 
AGTGGAAACG CCTTCCATGA ACAGCCCTGT AAGGCCCCGC AGTATGGGAA CTGTCTCAAC 
AGGCAGCCAG TGGCCCCTGG TGCACTCGAC GGTGCCTGTG GTGCCGGGAT TCAAGCCTCA 
AAGCTGAAGA GCACCCCCAT GCAAGGGAGC GGGGGCCAGC TGAATTTCGG CCTGCCGGTA 
GCGCCAAATG AGTCAGCTGG CAGCATGGTG AATGGCATGC AGAACCAGGA CCCAGTGGGA 
CAGGGGTACC TGGCTCACCA GCTCCTCGGC GACAGCATGC AGCACCCGGG GGCAGGCCGC 
CCCGGTCAGC AGATGCTTGG GCAGATTAGT GCTACCTCAC ACATCAACAT CTACCAAGGG 
CCAGAGAGCT GCCTGCCAGG GGCTCACGGC ATGGGCAGCC AGCCGTCAAG CTTGGCAGTT 
GTCAGGGGCT ACCAGCCATG TGCCAGCTTT GGGGGCAGCA GGCGCCAGGC TATGCCGAGG 
GACAGCCTTG CTCTGCAGTC AGGACAGCTC AGTGACACAA GTCAGACCTG CAGGGTGAAT 
GGTATCAAGA TGGAGATGAA AGGGCAGCCC CATCCGCTGT GCTCTAATCT GCAGAATTAC 
TCTGGTCAGT TCTATGACCA AACCGTGGGC TTCAGTCAGC AAGACACGAA AGCTGGTTCA 
TTCTCTATTT CAGACGCCAG CTGCCTGCTA CAGGGGACCA GCGCCAAAAA CTCTGAGTTA 
CTTTCCCCAG GTGCTAATCA GGTGACAAGC ACAGTGGACA GCCTCGACAG CCATGACCTG 
GAAGGGGTAC AGATTGACTT CGATGCCATC ATAGACGATG GGGACCACTC CAGCCTGATG 
TCGGGGGCCC TGAGCCCAAG TATCATTCAG AACCTTTCCC ATAGCTCCTC CCGCCTCACC 
ACGCCTCGGG CGTCCCTCCC ATTCCCAGCG CTGTCCATGA GCACCACCAA CATGGCTATC 
GGGGACATGA GTTCTTTGCT GACCTCCCTA GCGGAAGAAA GCAAATTCCT TGCAGTTATG 
CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_017011997.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_016867486.1 Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

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CloneID	OHu97167
Clone ID Related Accession (Same CDS sequence)	XM_017011997.1
Accession Version	XM_017011997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCCGC GCTGTCCATC CCTGCTGCTG CACAAACGAC TCAGCCACAG ATCCTCTTGG 
ATGGGCTACC TTGAAAGGGG GAAGCTCCAA AGAATGCGTA CAAACCCATA CATTTACCTA 
GAGGATGAAA GTCCTGGACA GACTTATCAC AGAGAGAGAA GAAACGCAAT CACTATGCAG 
CCACAGAATG TCCAGGGGCT CAGCAAAGTC AGTGAGGAAC CTTCAACATC GAGTGACGAG 
AGGGCCTCAT TGATCAAGAA AGAGATCCAT GGGTCCCTGC CACACGTGGC GGAGCCCTCT 
GTGCCGTACC GCGGGACGGT GTTTGCCATG GACCCCAGGA ATGGTTACAT GGAGCCCCAC 
TACCACCCTC CTCATCTTTT CCCTGCCTTC CATCCTCCTG TACCAATTGA TGCCAGACAT 
CATGAGGGCC GTTACCATTA CGATCCATCT CCGATTCCTC CATTGCATAT GACTTCCGCC 
TTATCTAGTA GCCCTACGTA TCCGGACCTG CCCTTCATTA GGATCTCCCC ACACCGGAAC 
CCCACTGCTG CTTCCGAGTC TCCCTTCAGC CCTCCACATC CCTACATTAA TCCCTACATG 
GACTATATCC GCTCCTTGCA CAGCAGCCCA TCGCTCTCCA TGATCTCAGC AACCCGTGGG 
CTGAGCCCTA CAGATGCGCC CCATGCAGGA GTCAGCCCAG CAGAATACTA TCATCAGATG 
GCCCTGCTAA CTGGCCAGCG CAGCCCCTAT GCAGACATTA TTCCCTCAGC TGCCACCGCC 
GGCACGGGGG CCATCCACAT GGAATATCTT CATGCTATGG ATAGCACCAG ATTCTCCAGC 
CCCAGGCTGT CAGCCAGGCC GAGCCGAAAA CGTACACTGT CCATATCACC ACTCTCCGAT 
CATAGCTTTG ACCTTCAGAC CATGATAAGG ACGTCTCCCA ACTCCTTGGT CACGATTCTC 
AATAATTCCC GTAGCAGCTC TTCAGCAAGT GGCTCCTATG GTCACTTATC TGCAAGTGCA 
ATCAGCCCTG CCTTGAGCTT CACCTACTCT TCCGCGCCCG TCTCTCTCCA CATGCATCAG 
CAGATCCTAA GCCGACAACA GAGCTTAGGT TCAGCCTTTG GACACAGCCC TCCACTCATC 
CACCCTGCCC CAACTTTTCC AACACAGAGG CCTATTCCAG GGATCCCTAC GGTTCTGAAC 
CCCGTCCAGG TCAGCTCCGG CCCTTCTGAG TCCTCACAGA ACAAGCCCAC GAGTGAGTCT 
GCAGTGAGCA GCACTGGTGA CCCGATGCAC AACAAGAGGT CCAAGATCAA ACCCGATGAA 
GACCTCCCCA GCCCAGGGGC TCGGGGGCAG CAGGAACAGC CCGAAGGAAC AACCCTTGTC 
AAGGAGGAAG GGGACAAAGA TGAAAGCAAA CAGGAGCCTG AAGTCATCTA TGAGACAAAC 
TGCCACTGGG AAGGCTGCGC GAGGGAGTTC GACACCCAAG AGCAGCTTGT GCACCATATA 
AATAACGACC ATATTCATGG AGAGAAGAAG GAGTTCGTGT GCAGGTGGCT GGACTGCTCA 
AGAGAGCAGA AACCCTTCAA AGCCCAGTAT ATGTTGGTAG TGCATATGAG AAGACACACG 
GGCGAGAAGC CTCACAAATG CACTTTTGAA GGTTGCACAA AGGCCTACTC GAGACTAGAA 
AACTTGAAAA CACACTTGAG ATCTCACACT GGAGAGAAAC CATACGTCTG TGAGCACGAA 
GGTTGCAACA AGGCTTTCTC AAATGCCTCT GATCGCGCCA AACACCAAAA CAGAACGCAT 
TCCAATGAGA AACCATATGT GTGCAAAATC CCAGGCTGCA CTAAGCGTTA CACAGACCCA 
AGCTCCCTCC GGAAACATGT GAAGACAGTG CATGGCCCAG AGGCTCATGT CACCAAGAAG 
CAGCGAGGGG ACATCCATCC TCGGCCGCCA CCCCCGAGAG ATTCCGGCAG CCATTCACAG 
TCCAGGTCGC CTGGCCGACC GACTCAGGGA GCCCTTGGTG AGCAGCAGGA CCTCAGCAAC 
ACTACCTCAA AGCGGGAAGA ATGCCTCCAG GTGAAAACCG TCAAGGCAGA GAAGCCAATG 
ACATCTCAGC CAAGCCCTGG TGGTCAGTCT TCATGCAGCA GCCAACAGTC CCCCATCAGC 
AACTATTCCA ACAGTGGGCT CGAGCTTCCT CTGACCGATG GAGGTAGTAT AGGAGACCTC 
AGTGCCATCG ATGAAACCCC AATCATGGAC TCAACCATTT CCACTGCAAC CACAGCCCTT 
GCTTTGCAAG CCAGGAGAAA CCCGGCAGGG ACCAAATGGA TGGAGCACGT AAAACTAGAA 
AGGCTAAAAC AAGTGAATGG AATGTTTCCG CGACTGAACC CCATTCTACC CCCTAAAGCC 
CCTGCGGTCT CTCCTCTCAT AGGAAATGGC ACACAGTCCA ACAACACCTG CAGCTTGGGT 
GGGCCCATGA CGCTTCTCCC GGGCAGAAGC GACCTCTCTG GGGTGGACGT CACTATGCTG 
AACATGCTCA ACAGAAGGGA CAGCAGCGCC AGCACCATCA GCTCGGCCTA CCTGAGCAGC 
CGCCGCTCCT CAGGGATCTC GCCCTGCTTC TCCAGCCGCC GCTCCAGCGA GGCGTCACAG 
GCCGAGGGCC GGCCGCAGAA CGTGAGCGTG GCCGACTCCT ACGACCCCAT CTCCACCGAC 
GCCTCGCGCC GCTCCAGCGA AGCCAGCCAG AGCGACGGCC TGCCCAGCCT GCTCAGCCTC 
ACGCCCGCCC AGCAGTACCG CCTCAAGGCC AAGTACGCGG CTGCCACAGG AGGGCCGCCG 
CCGACGCCCC TGCCCAACAT GGAGAGGATG AGCCTGAAGA CGCGCCTGGC GCTGCTCGGG 
GATGCCCTCG AGCCTGGCGT GGCCCTGCCT CCAGTTCATG CCCCGAGGAG GTGCAGCGAC 
GGGGGAGCCC ACGGCTACGG GCGGCGCCAC CTGCAGCCGC ACGATGCGCC GGGCCACGGC 
GTGAGGAGGG CCAGCGACCC GGTGCGGACA GGCTCCGAGG GCCTGGCCCT GCCTCGTGTG 
CCGCGCTTCA GCAGCCTCAG CAGCTGCAAC CCCCCGGCGA TGGCCACGTC CGCGGAGAAG 
CGCAGTCTCG TGCTTCAGAA TTACACGCGG CCCGAGGGCG GCCAGTCCCG AAACTTCCAC 
TCGTCCCCCT GTCCTCCCAG CATCACCGAG AACGTCACCC TGGAGTCCCT GACCATGGAC 
GCTGATGCCA ACCTGAACGA TGAGGATTTC CTGCCGGACG ACGTGGTGCA GTATTTAAAT 
TCCCAGAACC AAGCAGGGTA CGAGCAGCAC TTCCCCAGCG CCCTCCCGGA CGACAGCAAA 
GTGCCCCACG GGCCCGGTGA CTTTGACGCG CCCGGGCTGC CAGACAGCCA CGCTGGCCAG 
CAGTTCCATG CCCTCGAGCA GCCCTGCCCC GAGGGCAGCA AAACCGACCT GCCCATTCAG 
TGGAACGAAG TCAGCTCCGG AAGCGCCGAC CTGTCCTCCT CCAAGCTCAA GTGTGGGCCG 
CGGCCCGCTG TGCCGCAGAC TCGCGCCTTT GGGTTCTGCA ACGGCATGGT CGTCCACCCG 
CAGAACCCCT TGAGGAGCGG GCCTGCTGGG GGCTATCAGA CCCTCGGGGA GAACAGCAAC 
CCCTACGGTG GCCCAGAGCA CTTGATGCTC CACAACAGCC CCGGAAGTGG CACCAGTGGA 
AACGCCTTCC ATGAACAGCC CTGTAAGGCC CCGCAGTATG GGAACTGTCT CAACAGGCAG 
CCAGTGGCCC CTGGTGCACT CGACGGTGCC TGTGGTGCCG GGATTCAAGC CTCAAAGCTG 
AAGAGCACCC CCATGCAAGG GAGCGGGGGC CAGCTGAATT TCGGCCTGCC GGTAGCGCCA 
AATGAGTCAG CTGGCAGCAT GGTGAATGGC ATGCAGAACC AGGACCCAGT GGGACAGGGG 
TACCTGGCTC ACCAGCTCCT CGGCGACAGC ATGCAGCACC CGGGGGCAGG CCGCCCCGGT 
CAGCAGATGC TTGGGCAGAT TAGTGCTACC TCACACATCA ACATCTACCA AGGGCCAGAG 
AGCTGCCTGC CAGGGGCTCA CGGCATGGGC AGCCAGCCGT CAAGCTTGGC AGTTGTCAGG 
GGCTACCAGC CATGTGCCAG CTTTGGGGGC AGCAGGCGCC AGGCTATGCC GAGGGACAGC 
CTTGCTCTGC AGTCAGGACA GCTCAGTGAC ACAAGTCAGA CCTGCAGGGT GAATGGTATC 
AAGATGGAGA TGAAAGGGCA GCCCCATCCG CTGTGCTCTA ATCTGCAGAA TTACTCTGGT 
CAGTTCTATG ACCAAACCGT GGGCTTCAGT CAGCAAGACA CGAAAGCTGG TTCATTCTCT 
ATTTCAGACG CCAGCTGCCT GCTACAGGGG ACCAGCGCCA AAAACTCTGA GTTACTTTCC 
CCAGGTGCTA ATCAGGTGAC AAGCACAGTG GACAGCCTCG ACAGCCATGA CCTGGAAGGG 
GTACAGATTG ACTTCGATGC CATCATAGAC GATGGGGACC ACTCCAGCCT GATGTCGGGG 
GCCCTGAGCC CAAGTATCAT TCAGAACCTT TCCCATAGCT CCTCCCGCCT CACCACGCCT 
CGGGCGTCCC TCCCATTCCC AGCGCTGTCC ATGAGCACCA CCAACATGGC TATCGGGGAC 
ATGAGTTCTT TGCTGACCTC CCTAGCGGAA GAAAGCAAAT TCCTTGCAGT TATGCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867486.1
CDS	2752..7491
Translation	MHPRCPSLLLHKRLSHRSSWMGYLERGKLQRMRTNPYIYLEDESPGQTYHRERRNAITMQPQNVQGLSKVSEEPSTSSDERASLIKKEIHGSLPHVAEPSVPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHMTSALSSSPTYPDLPFIRISPHRNPTAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISATRGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADIIPSAATAGTGAIHMEYLHAMDSTRFSSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYSSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGARGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCAREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRPTQGALGEQQDLSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSIGDLSAIDETPIMDSTISTATTALALQARRNPAGTKWMEHVKLERLKQVNGMFPRLNPILPPKAPAVSPLIGNGTQSNNTCSLGGPMTLLPGRSDLSGVDVTMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQSDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRLALLGDALEPGVALPPVHAPRRCSDGGAHGYGRRHLQPHDAPGHGVRRASDPVRTGSEGLALPRVPRFSSLSSCNPPAMATSAEKRSLVLQNYTRPEGGQSRNFHSSPCPPSITENVTLESLTMDADANLNDEDFLPDDVVQYLNSQNQAGYEQHFPSALPDDSKVPHGPGDFDAPGLPDSHAGQQFHALEQPCPEGSKTDLPIQWNEVSSGSADLSSSKLKCGPRPAVPQTRAFGFCNGMVVHPQNPLRSGPAGGYQTLGENSNPYGGPEHLMLHNSPGSGTSGNAFHEQPCKAPQYGNCLNRQPVAPGALDGACGAGIQASKLKSTPMQGSGGQLNFGLPVAPNESAGSMVNGMQNQDPVGQGYLAHQLLGDSMQHPGAGRPGQQMLGQISATSHINIYQGPESCLPGAHGMGSQPSSLAVVRGYQPCASFGGSRRQAMPRDSLALQSGQLSDTSQTCRVNGIKMEMKGQPHPLCSNLQNYSGQFYDQTVGFSQQDTKAGSFSISDASCLLQGTSAKNSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHSSLMSGALSPSIIQNLSHSSSRLTTPRASLPFPALSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_017011997.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011997.1

ORF Insert Sequence:

ATGCATCCGC GCTGTCCATC CCTGCTGCTG CACAAACGAC TCAGCCACAG ATCCTCTTGG 
ATGGGCTACC TTGAAAGGGG GAAGCTCCAA AGAATGCGTA CAAACCCATA CATTTACCTA 
GAGGATGAAA GTCCTGGACA GACTTATCAC AGAGAGAGAA GAAACGCAAT CACTATGCAG 
CCACAGAATG TCCAGGGGCT CAGCAAAGTC AGTGAGGAAC CTTCAACATC GAGTGACGAG 
AGGGCCTCAT TGATCAAGAA AGAGATCCAT GGGTCCCTGC CACACGTGGC GGAGCCCTCT 
GTGCCGTACC GCGGGACGGT GTTTGCCATG GACCCCAGGA ATGGTTACAT GGAGCCCCAC 
TACCACCCTC CTCATCTTTT CCCTGCCTTC CATCCTCCTG TACCAATTGA TGCCAGACAT 
CATGAGGGCC GTTACCATTA CGATCCATCT CCGATTCCTC CATTGCATAT GACTTCCGCC 
TTATCTAGTA GCCCTACGTA TCCGGACCTG CCCTTCATTA GGATCTCCCC ACACCGGAAC 
CCCACTGCTG CTTCCGAGTC TCCCTTCAGC CCTCCACATC CCTACATTAA TCCCTACATG 
GACTATATCC GCTCCTTGCA CAGCAGCCCA TCGCTCTCCA TGATCTCAGC AACCCGTGGG 
CTGAGCCCTA CAGATGCGCC CCATGCAGGA GTCAGCCCAG CAGAATACTA TCATCAGATG 
GCCCTGCTAA CTGGCCAGCG CAGCCCCTAT GCAGACATTA TTCCCTCAGC TGCCACCGCC 
GGCACGGGGG CCATCCACAT GGAATATCTT CATGCTATGG ATAGCACCAG ATTCTCCAGC 
CCCAGGCTGT CAGCCAGGCC GAGCCGAAAA CGTACACTGT CCATATCACC ACTCTCCGAT 
CATAGCTTTG ACCTTCAGAC CATGATAAGG ACGTCTCCCA ACTCCTTGGT CACGATTCTC 
AATAATTCCC GTAGCAGCTC TTCAGCAAGT GGCTCCTATG GTCACTTATC TGCAAGTGCA 
ATCAGCCCTG CCTTGAGCTT CACCTACTCT TCCGCGCCCG TCTCTCTCCA CATGCATCAG 
CAGATCCTAA GCCGACAACA GAGCTTAGGT TCAGCCTTTG GACACAGCCC TCCACTCATC 
CACCCTGCCC CAACTTTTCC AACACAGAGG CCTATTCCAG GGATCCCTAC GGTTCTGAAC 
CCCGTCCAGG TCAGCTCCGG CCCTTCTGAG TCCTCACAGA ACAAGCCCAC GAGTGAGTCT 
GCAGTGAGCA GCACTGGTGA CCCGATGCAC AACAAGAGGT CCAAGATCAA ACCCGATGAA 
GACCTCCCCA GCCCAGGGGC TCGGGGGCAG CAGGAACAGC CCGAAGGAAC AACCCTTGTC 
AAGGAGGAAG GGGACAAAGA TGAAAGCAAA CAGGAGCCTG AAGTCATCTA TGAGACAAAC 
TGCCACTGGG AAGGCTGCGC GAGGGAGTTC GACACCCAAG AGCAGCTTGT GCACCATATA 
AATAACGACC ATATTCATGG AGAGAAGAAG GAGTTCGTGT GCAGGTGGCT GGACTGCTCA 
AGAGAGCAGA AACCCTTCAA AGCCCAGTAT ATGTTGGTAG TGCATATGAG AAGACACACG 
GGCGAGAAGC CTCACAAATG CACTTTTGAA GGTTGCACAA AGGCCTACTC GAGACTAGAA 
AACTTGAAAA CACACTTGAG ATCTCACACT GGAGAGAAAC CATACGTCTG TGAGCACGAA 
GGTTGCAACA AGGCTTTCTC AAATGCCTCT GATCGCGCCA AACACCAAAA CAGAACGCAT 
TCCAATGAGA AACCATATGT GTGCAAAATC CCAGGCTGCA CTAAGCGTTA CACAGACCCA 
AGCTCCCTCC GGAAACATGT GAAGACAGTG CATGGCCCAG AGGCTCATGT CACCAAGAAG 
CAGCGAGGGG ACATCCATCC TCGGCCGCCA CCCCCGAGAG ATTCCGGCAG CCATTCACAG 
TCCAGGTCGC CTGGCCGACC GACTCAGGGA GCCCTTGGTG AGCAGCAGGA CCTCAGCAAC 
ACTACCTCAA AGCGGGAAGA ATGCCTCCAG GTGAAAACCG TCAAGGCAGA GAAGCCAATG 
ACATCTCAGC CAAGCCCTGG TGGTCAGTCT TCATGCAGCA GCCAACAGTC CCCCATCAGC 
AACTATTCCA ACAGTGGGCT CGAGCTTCCT CTGACCGATG GAGGTAGTAT AGGAGACCTC 
AGTGCCATCG ATGAAACCCC AATCATGGAC TCAACCATTT CCACTGCAAC CACAGCCCTT 
GCTTTGCAAG CCAGGAGAAA CCCGGCAGGG ACCAAATGGA TGGAGCACGT AAAACTAGAA 
AGGCTAAAAC AAGTGAATGG AATGTTTCCG CGACTGAACC CCATTCTACC CCCTAAAGCC 
CCTGCGGTCT CTCCTCTCAT AGGAAATGGC ACACAGTCCA ACAACACCTG CAGCTTGGGT 
GGGCCCATGA CGCTTCTCCC GGGCAGAAGC GACCTCTCTG GGGTGGACGT CACTATGCTG 
AACATGCTCA ACAGAAGGGA CAGCAGCGCC AGCACCATCA GCTCGGCCTA CCTGAGCAGC 
CGCCGCTCCT CAGGGATCTC GCCCTGCTTC TCCAGCCGCC GCTCCAGCGA GGCGTCACAG 
GCCGAGGGCC GGCCGCAGAA CGTGAGCGTG GCCGACTCCT ACGACCCCAT CTCCACCGAC 
GCCTCGCGCC GCTCCAGCGA AGCCAGCCAG AGCGACGGCC TGCCCAGCCT GCTCAGCCTC 
ACGCCCGCCC AGCAGTACCG CCTCAAGGCC AAGTACGCGG CTGCCACAGG AGGGCCGCCG 
CCGACGCCCC TGCCCAACAT GGAGAGGATG AGCCTGAAGA CGCGCCTGGC GCTGCTCGGG 
GATGCCCTCG AGCCTGGCGT GGCCCTGCCT CCAGTTCATG CCCCGAGGAG GTGCAGCGAC 
GGGGGAGCCC ACGGCTACGG GCGGCGCCAC CTGCAGCCGC ACGATGCGCC GGGCCACGGC 
GTGAGGAGGG CCAGCGACCC GGTGCGGACA GGCTCCGAGG GCCTGGCCCT GCCTCGTGTG 
CCGCGCTTCA GCAGCCTCAG CAGCTGCAAC CCCCCGGCGA TGGCCACGTC CGCGGAGAAG 
CGCAGTCTCG TGCTTCAGAA TTACACGCGG CCCGAGGGCG GCCAGTCCCG AAACTTCCAC 
TCGTCCCCCT GTCCTCCCAG CATCACCGAG AACGTCACCC TGGAGTCCCT GACCATGGAC 
GCTGATGCCA ACCTGAACGA TGAGGATTTC CTGCCGGACG ACGTGGTGCA GTATTTAAAT 
TCCCAGAACC AAGCAGGGTA CGAGCAGCAC TTCCCCAGCG CCCTCCCGGA CGACAGCAAA 
GTGCCCCACG GGCCCGGTGA CTTTGACGCG CCCGGGCTGC CAGACAGCCA CGCTGGCCAG 
CAGTTCCATG CCCTCGAGCA GCCCTGCCCC GAGGGCAGCA AAACCGACCT GCCCATTCAG 
TGGAACGAAG TCAGCTCCGG AAGCGCCGAC CTGTCCTCCT CCAAGCTCAA GTGTGGGCCG 
CGGCCCGCTG TGCCGCAGAC TCGCGCCTTT GGGTTCTGCA ACGGCATGGT CGTCCACCCG 
CAGAACCCCT TGAGGAGCGG GCCTGCTGGG GGCTATCAGA CCCTCGGGGA GAACAGCAAC 
CCCTACGGTG GCCCAGAGCA CTTGATGCTC CACAACAGCC CCGGAAGTGG CACCAGTGGA 
AACGCCTTCC ATGAACAGCC CTGTAAGGCC CCGCAGTATG GGAACTGTCT CAACAGGCAG 
CCAGTGGCCC CTGGTGCACT CGACGGTGCC TGTGGTGCCG GGATTCAAGC CTCAAAGCTG 
AAGAGCACCC CCATGCAAGG GAGCGGGGGC CAGCTGAATT TCGGCCTGCC GGTAGCGCCA 
AATGAGTCAG CTGGCAGCAT GGTGAATGGC ATGCAGAACC AGGACCCAGT GGGACAGGGG 
TACCTGGCTC ACCAGCTCCT CGGCGACAGC ATGCAGCACC CGGGGGCAGG CCGCCCCGGT 
CAGCAGATGC TTGGGCAGAT TAGTGCTACC TCACACATCA ACATCTACCA AGGGCCAGAG 
AGCTGCCTGC CAGGGGCTCA CGGCATGGGC AGCCAGCCGT CAAGCTTGGC AGTTGTCAGG 
GGCTACCAGC CATGTGCCAG CTTTGGGGGC AGCAGGCGCC AGGCTATGCC GAGGGACAGC 
CTTGCTCTGC AGTCAGGACA GCTCAGTGAC ACAAGTCAGA CCTGCAGGGT GAATGGTATC 
AAGATGGAGA TGAAAGGGCA GCCCCATCCG CTGTGCTCTA ATCTGCAGAA TTACTCTGGT 
CAGTTCTATG ACCAAACCGT GGGCTTCAGT CAGCAAGACA CGAAAGCTGG TTCATTCTCT 
ATTTCAGACG CCAGCTGCCT GCTACAGGGG ACCAGCGCCA AAAACTCTGA GTTACTTTCC 
CCAGGTGCTA ATCAGGTGAC AAGCACAGTG GACAGCCTCG ACAGCCATGA CCTGGAAGGG 
GTACAGATTG ACTTCGATGC CATCATAGAC GATGGGGACC ACTCCAGCCT GATGTCGGGG 
GCCCTGAGCC CAAGTATCAT TCAGAACCTT TCCCATAGCT CCTCCCGCCT CACCACGCCT 
CGGGCGTCCC TCCCATTCCC AGCGCTGTCC ATGAGCACCA CCAACATGGC TATCGGGGAC 
ATGAGTTCTT TGCTGACCTC CCTAGCGGAA GAAAGCAAAT TCCTTGCAGT TATGCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[A novel mutation of GLI3 gene underlying synpolydactyly in a family].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics34(4)490-493(2017 Aug)
Kang R,Huang S,Li J,Zou Y,Xu P,Gao M,Wang L,Xie H,Yan J,Gao Y

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オンライン注文

GLI3 cDNA ORF clone, Homo sapiens(Human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

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