GLI3 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015281556.1	XP_015137042.1	transcriptional activator GLI3 isoform X1
NM_001271903.1	NP_001258832.1	transcriptional activator GLI3
XM_015281557.1	XP_015137043.1	transcriptional activator GLI3 isoform X1
XM_015281561.1	XP_015137047.1	transcriptional activator GLI3 isoform X2
XM_015281561.2	XP_015137047.1	transcriptional activator GLI3 isoform X2
XM_015281557.2	XP_015137043.1	transcriptional activator GLI3 isoform X1
XM_015281558.1	XP_015137044.1	transcriptional activator GLI3 isoform X1
XM_015281556.2	XP_015137042.1	transcriptional activator GLI3 isoform X1
XM_015281558.2	XP_015137044.1	transcriptional activator GLI3 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa31660	XM_015281561.2 Latest version!	Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa00034	NM_001271903.1 Latest version!	Gallus gallus GLI family zinc finger 3 (GLI3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa00034	XM_015281558.2 Latest version!	Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa00034	XM_015281557.2 Latest version!	Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa00034	XM_015281556.2 Latest version!	Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OGa31660	XM_015281561.1	Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa00034	XM_015281557.1	Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa00034	XM_015281556.1	Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OGa00034	XM_015281558.1	Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

GLI3 ( XM_015281561.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137047.1 Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa31660
Clone ID Related Accession (Same CDS sequence)	XM_015281561.1 , XM_015281561.2
Accession Version	XM_015281561.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	transcriptional activator GLI3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGAACAA ACCCACAAGT 
GAGTCTGCAG TGAGCAGCAC TGGGGATCCA ATGCACAACA AGCGCTCCAA GATAAAGCCG 
GATGAGGACC TCCCCAGCCC GGGTGCGGGA AGCGTGCAGG AACAGCCAGA AGGTATGACC 
CCGGTAAAGG AGGAAGGGGA CAGAGATGAA AGCAAACAGG AGCCTGAAGT GGTCTACGAG 
ACGAACTGCC ATTGGGAAGG CTGTTCCCGG GAGTTTGACA CACAGGAACA GCTAGTGCAT 
CATATAAATA ATGACCACAT ACATGGTGAG AAGAAAGAGT TTGTGTGCCG ATGGCTGGAC 
TGTTCCCGGG AGCAGAAACC ATTCAAAGCC CAGTATATGC TGGTGGTCCA CATGAGGAGA 
CATACAGGGG AAAAGCCACA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCCAGA 
CTAGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA TGTGTGCGAA 
CACGAGGGCT GCAACAAAGC TTTCTCCAAT GCATCTGACA GGGCCAAGCA CCAAAACAGG 
ACTCATTCAA ATGAAAAACC ATATGTTTGC AAGATCCCAG GCTGCACAAA GCGCTATACA 
GACCCTAGTT CTCTCCGGAA ACATGTGAAG ACTGTGCATG GCCCAGAAGC ACACGTCACC 
AAGAAGCAGC GAGGAGACAT CCACCCAAGG CCTCCACCAC CCAGAGACCC AGGCAGCCAC 
TCTCAGACAC GATCACCAGG CCAGCAGACT CAGGGTGCGA CTGGGGAGCA GAAGGACCTC 
AACAGCACTA CCTCAAGGCG TGAAGAATGC CTCCAAGTGA AAGCAGTCAA GTCAGAGAAA 
CCAATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTACAT GCAGCAGCGA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAA TGGGATCGAG CTTACTCTGA CTGGTGGCGG TAGTGTAGGA 
GACCTCAGTG TCATCGATGA AACCCCAATC ATGGACTCTA CCATTTCCAC AGCCACCACA 
GCACTTGGCT TACAGGCCAG GAGGAATATG ACAGGGACCA AATGGATGGA GCAAGTGAAA 
TTAGAAAGGT TGAAACAAGT TAATGGAATG CTTCCAAGAC TGAACCCCGT TCCACCTTCC 
AAAGCCCCGA CCTTGCCGCC TCTCATAGGA AATGGTACCC AATCAAACAG CAGCTGCAGT 
GTAGGAGGAT CCATGACTAT TCTGCCAAAC AGGAATGAAC TTTCTAGTAC AGACATCACT 
GTGTTGAACA TGCTTAACAG GAGGGACAGC AATACGAGCA CAATCAGTTC AGCCTACTTG 
AGCAGCCGCA GGTCTTCTGG AATCTCACCT TGCTTCTCCA GCCGGAGGTC CAGTGATGCC 
TCTCAGGCTG AGGGAAGACC ACAAAATGTG AGCGTTGCAG ACTCCTATGA CCCCATTTCA 
ACGGATGCCT CTCGGCGCTC AAGTGAAGCA AGCCAATGTG ATGACCTGCC AAGTCTTCTC 
AGCCTCACCC CAGCCCAGCA ATACAGGCTG AAAGCTAAAT ATGCAGCAGC TACTGGTGGA 
CCCCCACCAA CTCCACTGCC TAATATGGAG AGGATGAGCC TCAAAACAAG GATGGCACTC 
TTGGGTGACT GCAGGGAGTC TGGAATATCT CCACTGCCTC CAGTGAATGC CCCTAGAAGA 
TGTAGTGATG GTGGGGCAAA TGGTTACAGC AGGAGGCATC TTCTATCTCA TGATGCTCTA 
GGAAATGGGA CAAGGAGAGC CAGTGATCCA GTAAGAATGG TCTCCAATAA CCTCTCTGTC 
CCTAGAGTCC AACATTTCAA CAGTCTTAAT AGCTTTAACT CTCCTGCTTT GCCTCCATCC 
ATGGAAAAGC GCAACCTTGT CCTTCAGAAC TGTACCCATT CTGAGGGTGG TGTTTTCCGT 
GGCTTCAGTT CCCCCTGTCC TCCAAGCATC AGCGAGAACG TCACCCTGGA GGCTGTTACG 
ATGGAAGCAG GTGGCAGTTT GAATGATGAG GATCTCCTGC CAGATGATGT GGTTCAGTAT 
CTGAATTCCC AGAACCAGGG CACGTGCGAC CACTTGTTGA ACAATGTCCT AGACAGCAAC 
AAAATGCATC ACAGTGTGGT TTTAGGAAAC AACAATCCCA GCAGCTTTGA TCGAGCCCCT 
CCAGCAAGCA GTCAGCCTGC AGGCTCAGAG GTAAGCAAGA GTGACTTACC CATTCAGTGG 
AATGAAGTAA GCTCAGGAAG TTCTGACTTA TCTCCTACAA AACTGAAATG TAGTCAGCGC 
TCAGCAGTGC AGCAGGCTAG AGCATTTGGA CTGTATAACA ACATGATCGT TCAGCAGCAG 
AACTTGCAGA GAGGCAATGT GTACCAGCAA AATGGCTATC AGAACCTGAT GGAAAACAAT 
GGTTCCTATA GTTTGCAGCA GAACACGGTC CTTGGCAGCG GAGCCAGTAG TTCTTTCGGC 
ATGCAGCCCA ATAAGCCTTA TGGTGAAAGC GTCAGCAGGC AACCTATGAT TTTTGGGGCA 
ATGGACAGTT CCTGTGGCAT AACAGTTCAA GGGCAGAAGC TAAGAAGCAG CAACATGCCA 
GTGAGTGGGA ACCAGCAAAA TTTTGGTCAT CCCATCGCAT CCAGTGATCA AGCCACCAGT 
ATGGCAAATG GGATGCAAAA CAGGAATGTG ATGGAACAGG AATATTTGCA AAACGAACTG 
GTTGGAGATG GCATTCATTA CCAGGGAGTC AATCAGTCCA GTCCAATGAC ACTAGGGCAG 
GTTAGTCCTA CCTCACAAAG CAGCCTCCAT CAGGGGCCAC AGAGTTGTCC TCCGGTATCT 
CATACCATTG GTAACCAGTC TTCAGGTTTG TCAGTGGCCA AGAGTTACCA GCCATGTGCT 
AATTACAGTG GCAACAGACG GCAAAATGTG TTGAGGAACA ACCTGGCACA ACAGCAAGGA 
CATGTAAGTG ATGGCAACCA GACGTACAGG GTAAACACCA TTAAATTGGA GATGCAAGGT 
CAATCACAGC AGTTCTGCTC TAATATGCAG AATTACTCTG GTCAGTTATA TGACCAAACC 
ACAGGCTTTA GCCACCAAGC TATGAAAATA GGTTCTTCTT TCTTTGTTTC GGAAGCTAAC 
TGCCTGCTGC AGGAGACTGC TACTGCAAAC TCCTCTGAGC TTCTTTCCCC GGGGGCTAAC 
CAAGTATCAA GCACAGTTGA CAGCCTTGAC AGCAACAGCC TAGAGGGTGT GCAGATTGAT 
TTTGATGCTA TCATAGATGA TGGAGACCAT GTCAGCTTAA TTTCAGGAGC CCTGAGCCCA 
AGCATCATTC AGAATCTCTC CCGCAATTCC TCACGCCTCA CCACTCCCCG AGCGTCTCTT 
ACCTTCCCAG CTATGCCTGT AAGCACAACC AACATGGCTA TTGGGGACAT GAGTTCTTTG 
TTGACCTCAC TTGCAGAAGA AAGCAAGTTT CTTGCTGTTA TGCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137047.1
CDS	129..4856
Translation	MEAQSHSSTTTEKKKVENSIVKCSNRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQGGQGLSKISEEPSTSSEERASLIKKEIHGSISHLPEPSVPYRGTLFTMDPRNGYMDPHYHPPHLFPAFHPPVPIDARHHEGRYHYEPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASESPFSTPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGALHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPPTPVSLQQMHQQIISRQQTLGSAFGHSPPLIHPAPTFPTQRPIPGIPSVLNPVQVSSGPSESTQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGAGSVQEQPEGMTPVKEEGDRDESKQEPEVVYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDPGSHSQTRSPGQQTQGATGEQKDLNSTTSRREECLQVKAVKSEKPMTSQPSPGGQSTCSSEQSPISNYSNNGIELTLTGGGSVGDLSVIDETPIMDSTISTATTALGLQARRNMTGTKWMEQVKLERLKQVNGMLPRLNPVPPSKAPTLPPLIGNGTQSNSSCSVGGSMTILPNRNELSSTDITVLNMLNRRDSNTSTISSAYLSSRRSSGISPCFSSRRSSDASQAEGRPQNVSVADSYDPISTDASRRSSEASQCDDLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDCRESGISPLPPVNAPRRCSDGGANGYSRRHLLSHDALGNGTRRASDPVRMVSNNLSVPRVQHFNSLNSFNSPALPPSMEKRNLVLQNCTHSEGGVFRGFSSPCPPSISENVTLEAVTMEAGGSLNDEDLLPDDVVQYLNSQNQGTCDHLLNNVLDSNKMHHSVVLGNNNPSSFDRAPPASSQPAGSEVSKSDLPIQWNEVSSGSSDLSPTKLKCSQRSAVQQARAFGLYNNMIVQQQNLQRGNVYQQNGYQNLMENNGSYSLQQNTVLGSGASSSFGMQPNKPYGESVSRQPMIFGAMDSSCGITVQGQKLRSSNMPVSGNQQNFGHPIASSDQATSMANGMQNRNVMEQEYLQNELVGDGIHYQGVNQSSPMTLGQVSPTSQSSLHQGPQSCPPVSHTIGNQSSGLSVAKSYQPCANYSGNRRQNVLRNNLAQQQGHVSDGNQTYRVNTIKLEMQGQSQQFCSNMQNYSGQLYDQTTGFSHQAMKIGSSFFVSEANCLLQETATANSSELLSPGANQVSSTVDSLDSNSLEGVQIDFDAIIDDGDHVSLISGALSPSIIQNLSRNSSRLTTPRASLTFPAMPVSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_015281561.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281561.1

ORF Insert Sequence:

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGAACAA ACCCACAAGT 
GAGTCTGCAG TGAGCAGCAC TGGGGATCCA ATGCACAACA AGCGCTCCAA GATAAAGCCG 
GATGAGGACC TCCCCAGCCC GGGTGCGGGA AGCGTGCAGG AACAGCCAGA AGGTATGACC 
CCGGTAAAGG AGGAAGGGGA CAGAGATGAA AGCAAACAGG AGCCTGAAGT GGTCTACGAG 
ACGAACTGCC ATTGGGAAGG CTGTTCCCGG GAGTTTGACA CACAGGAACA GCTAGTGCAT 
CATATAAATA ATGACCACAT ACATGGTGAG AAGAAAGAGT TTGTGTGCCG ATGGCTGGAC 
TGTTCCCGGG AGCAGAAACC ATTCAAAGCC CAGTATATGC TGGTGGTCCA CATGAGGAGA 
CATACAGGGG AAAAGCCACA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCCAGA 
CTAGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA TGTGTGCGAA 
CACGAGGGCT GCAACAAAGC TTTCTCCAAT GCATCTGACA GGGCCAAGCA CCAAAACAGG 
ACTCATTCAA ATGAAAAACC ATATGTTTGC AAGATCCCAG GCTGCACAAA GCGCTATACA 
GACCCTAGTT CTCTCCGGAA ACATGTGAAG ACTGTGCATG GCCCAGAAGC ACACGTCACC 
AAGAAGCAGC GAGGAGACAT CCACCCAAGG CCTCCACCAC CCAGAGACCC AGGCAGCCAC 
TCTCAGACAC GATCACCAGG CCAGCAGACT CAGGGTGCGA CTGGGGAGCA GAAGGACCTC 
AACAGCACTA CCTCAAGGCG TGAAGAATGC CTCCAAGTGA AAGCAGTCAA GTCAGAGAAA 
CCAATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTACAT GCAGCAGCGA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAA TGGGATCGAG CTTACTCTGA CTGGTGGCGG TAGTGTAGGA 
GACCTCAGTG TCATCGATGA AACCCCAATC ATGGACTCTA CCATTTCCAC AGCCACCACA 
GCACTTGGCT TACAGGCCAG GAGGAATATG ACAGGGACCA AATGGATGGA GCAAGTGAAA 
TTAGAAAGGT TGAAACAAGT TAATGGAATG CTTCCAAGAC TGAACCCCGT TCCACCTTCC 
AAAGCCCCGA CCTTGCCGCC TCTCATAGGA AATGGTACCC AATCAAACAG CAGCTGCAGT 
GTAGGAGGAT CCATGACTAT TCTGCCAAAC AGGAATGAAC TTTCTAGTAC AGACATCACT 
GTGTTGAACA TGCTTAACAG GAGGGACAGC AATACGAGCA CAATCAGTTC AGCCTACTTG 
AGCAGCCGCA GGTCTTCTGG AATCTCACCT TGCTTCTCCA GCCGGAGGTC CAGTGATGCC 
TCTCAGGCTG AGGGAAGACC ACAAAATGTG AGCGTTGCAG ACTCCTATGA CCCCATTTCA 
ACGGATGCCT CTCGGCGCTC AAGTGAAGCA AGCCAATGTG ATGACCTGCC AAGTCTTCTC 
AGCCTCACCC CAGCCCAGCA ATACAGGCTG AAAGCTAAAT ATGCAGCAGC TACTGGTGGA 
CCCCCACCAA CTCCACTGCC TAATATGGAG AGGATGAGCC TCAAAACAAG GATGGCACTC 
TTGGGTGACT GCAGGGAGTC TGGAATATCT CCACTGCCTC CAGTGAATGC CCCTAGAAGA 
TGTAGTGATG GTGGGGCAAA TGGTTACAGC AGGAGGCATC TTCTATCTCA TGATGCTCTA 
GGAAATGGGA CAAGGAGAGC CAGTGATCCA GTAAGAATGG TCTCCAATAA CCTCTCTGTC 
CCTAGAGTCC AACATTTCAA CAGTCTTAAT AGCTTTAACT CTCCTGCTTT GCCTCCATCC 
ATGGAAAAGC GCAACCTTGT CCTTCAGAAC TGTACCCATT CTGAGGGTGG TGTTTTCCGT 
GGCTTCAGTT CCCCCTGTCC TCCAAGCATC AGCGAGAACG TCACCCTGGA GGCTGTTACG 
ATGGAAGCAG GTGGCAGTTT GAATGATGAG GATCTCCTGC CAGATGATGT GGTTCAGTAT 
CTGAATTCCC AGAACCAGGG CACGTGCGAC CACTTGTTGA ACAATGTCCT AGACAGCAAC 
AAAATGCATC ACAGTGTGGT TTTAGGAAAC AACAATCCCA GCAGCTTTGA TCGAGCCCCT 
CCAGCAAGCA GTCAGCCTGC AGGCTCAGAG GTAAGCAAGA GTGACTTACC CATTCAGTGG 
AATGAAGTAA GCTCAGGAAG TTCTGACTTA TCTCCTACAA AACTGAAATG TAGTCAGCGC 
TCAGCAGTGC AGCAGGCTAG AGCATTTGGA CTGTATAACA ACATGATCGT TCAGCAGCAG 
AACTTGCAGA GAGGCAATGT GTACCAGCAA AATGGCTATC AGAACCTGAT GGAAAACAAT 
GGTTCCTATA GTTTGCAGCA GAACACGGTC CTTGGCAGCG GAGCCAGTAG TTCTTTCGGC 
ATGCAGCCCA ATAAGCCTTA TGGTGAAAGC GTCAGCAGGC AACCTATGAT TTTTGGGGCA 
ATGGACAGTT CCTGTGGCAT AACAGTTCAA GGGCAGAAGC TAAGAAGCAG CAACATGCCA 
GTGAGTGGGA ACCAGCAAAA TTTTGGTCAT CCCATCGCAT CCAGTGATCA AGCCACCAGT 
ATGGCAAATG GGATGCAAAA CAGGAATGTG ATGGAACAGG AATATTTGCA AAACGAACTG 
GTTGGAGATG GCATTCATTA CCAGGGAGTC AATCAGTCCA GTCCAATGAC ACTAGGGCAG 
GTTAGTCCTA CCTCACAAAG CAGCCTCCAT CAGGGGCCAC AGAGTTGTCC TCCGGTATCT 
CATACCATTG GTAACCAGTC TTCAGGTTTG TCAGTGGCCA AGAGTTACCA GCCATGTGCT 
AATTACAGTG GCAACAGACG GCAAAATGTG TTGAGGAACA ACCTGGCACA ACAGCAAGGA 
CATGTAAGTG ATGGCAACCA GACGTACAGG GTAAACACCA TTAAATTGGA GATGCAAGGT 
CAATCACAGC AGTTCTGCTC TAATATGCAG AATTACTCTG GTCAGTTATA TGACCAAACC 
ACAGGCTTTA GCCACCAAGC TATGAAAATA GGTTCTTCTT TCTTTGTTTC GGAAGCTAAC 
TGCCTGCTGC AGGAGACTGC TACTGCAAAC TCCTCTGAGC TTCTTTCCCC GGGGGCTAAC 
CAAGTATCAA GCACAGTTGA CAGCCTTGAC AGCAACAGCC TAGAGGGTGT GCAGATTGAT 
TTTGATGCTA TCATAGATGA TGGAGACCAT GTCAGCTTAA TTTCAGGAGC CCTGAGCCCA 
AGCATCATTC AGAATCTCTC CCGCAATTCC TCACGCCTCA CCACTCCCCG AGCGTCTCTT 
ACCTTCCCAG CTATGCCTGT AAGCACAACC AACATGGCTA TTGGGGACAT GAGTTCTTTG 
TTGACCTCAC TTGCAGAAGA AAGCAAGTTT CTTGCTGTTA TGCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_015281561.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137047.1 Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa31660
Clone ID Related Accession (Same CDS sequence)	XM_015281561.1 , XM_015281561.2
Accession Version	XM_015281561.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	transcriptional activator GLI3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281561.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGAACAA ACCCACAAGT 
GAGTCTGCAG TGAGCAGCAC TGGGGATCCA ATGCACAACA AGCGCTCCAA GATAAAGCCG 
GATGAGGACC TCCCCAGCCC GGGTGCGGGA AGCGTGCAGG AACAGCCAGA AGGTATGACC 
CCGGTAAAGG AGGAAGGGGA CAGAGATGAA AGCAAACAGG AGCCTGAAGT GGTCTACGAG 
ACGAACTGCC ATTGGGAAGG CTGTTCCCGG GAGTTTGACA CACAGGAACA GCTAGTGCAT 
CATATAAATA ATGACCACAT ACATGGTGAG AAGAAAGAGT TTGTGTGCCG ATGGCTGGAC 
TGTTCCCGGG AGCAGAAACC ATTCAAAGCC CAGTATATGC TGGTGGTCCA CATGAGGAGA 
CATACAGGGG AAAAGCCACA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCCAGA 
CTAGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA TGTGTGCGAA 
CACGAGGGCT GCAACAAAGC TTTCTCCAAT GCATCTGACA GGGCCAAGCA CCAAAACAGG 
ACTCATTCAA ATGAAAAACC ATATGTTTGC AAGATCCCAG GCTGCACAAA GCGCTATACA 
GACCCTAGTT CTCTCCGGAA ACATGTGAAG ACTGTGCATG GCCCAGAAGC ACACGTCACC 
AAGAAGCAGC GAGGAGACAT CCACCCAAGG CCTCCACCAC CCAGAGACCC AGGCAGCCAC 
TCTCAGACAC GATCACCAGG CCAGCAGACT CAGGGTGCGA CTGGGGAGCA GAAGGACCTC 
AACAGCACTA CCTCAAGGCG TGAAGAATGC CTCCAAGTGA AAGCAGTCAA GTCAGAGAAA 
CCAATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTACAT GCAGCAGCGA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAA TGGGATCGAG CTTACTCTGA CTGGTGGCGG TAGTGTAGGA 
GACCTCAGTG TCATCGATGA AACCCCAATC ATGGACTCTA CCATTTCCAC AGCCACCACA 
GCACTTGGCT TACAGGCCAG GAGGAATATG ACAGGGACCA AATGGATGGA GCAAGTGAAA 
TTAGAAAGGT TGAAACAAGT TAATGGAATG CTTCCAAGAC TGAACCCCGT TCCACCTTCC 
AAAGCCCCGA CCTTGCCGCC TCTCATAGGA AATGGTACCC AATCAAACAG CAGCTGCAGT 
GTAGGAGGAT CCATGACTAT TCTGCCAAAC AGGAATGAAC TTTCTAGTAC AGACATCACT 
GTGTTGAACA TGCTTAACAG GAGGGACAGC AATACGAGCA CAATCAGTTC AGCCTACTTG 
AGCAGCCGCA GGTCTTCTGG AATCTCACCT TGCTTCTCCA GCCGGAGGTC CAGTGATGCC 
TCTCAGGCTG AGGGAAGACC ACAAAATGTG AGCGTTGCAG ACTCCTATGA CCCCATTTCA 
ACGGATGCCT CTCGGCGCTC AAGTGAAGCA AGCCAATGTG ATGACCTGCC AAGTCTTCTC 
AGCCTCACCC CAGCCCAGCA ATACAGGCTG AAAGCTAAAT ATGCAGCAGC TACTGGTGGA 
CCCCCACCAA CTCCACTGCC TAATATGGAG AGGATGAGCC TCAAAACAAG GATGGCACTC 
TTGGGTGACT GCAGGGAGTC TGGAATATCT CCACTGCCTC CAGTGAATGC CCCTAGAAGA 
TGTAGTGATG GTGGGGCAAA TGGTTACAGC AGGAGGCATC TTCTATCTCA TGATGCTCTA 
GGAAATGGGA CAAGGAGAGC CAGTGATCCA GTAAGAATGG TCTCCAATAA CCTCTCTGTC 
CCTAGAGTCC AACATTTCAA CAGTCTTAAT AGCTTTAACT CTCCTGCTTT GCCTCCATCC 
ATGGAAAAGC GCAACCTTGT CCTTCAGAAC TGTACCCATT CTGAGGGTGG TGTTTTCCGT 
GGCTTCAGTT CCCCCTGTCC TCCAAGCATC AGCGAGAACG TCACCCTGGA GGCTGTTACG 
ATGGAAGCAG GTGGCAGTTT GAATGATGAG GATCTCCTGC CAGATGATGT GGTTCAGTAT 
CTGAATTCCC AGAACCAGGG CACGTGCGAC CACTTGTTGA ACAATGTCCT AGACAGCAAC 
AAAATGCATC ACAGTGTGGT TTTAGGAAAC AACAATCCCA GCAGCTTTGA TCGAGCCCCT 
CCAGCAAGCA GTCAGCCTGC AGGCTCAGAG GTAAGCAAGA GTGACTTACC CATTCAGTGG 
AATGAAGTAA GCTCAGGAAG TTCTGACTTA TCTCCTACAA AACTGAAATG TAGTCAGCGC 
TCAGCAGTGC AGCAGGCTAG AGCATTTGGA CTGTATAACA ACATGATCGT TCAGCAGCAG 
AACTTGCAGA GAGGCAATGT GTACCAGCAA AATGGCTATC AGAACCTGAT GGAAAACAAT 
GGTTCCTATA GTTTGCAGCA GAACACGGTC CTTGGCAGCG GAGCCAGTAG TTCTTTCGGC 
ATGCAGCCCA ATAAGCCTTA TGGTGAAAGC GTCAGCAGGC AACCTATGAT TTTTGGGGCA 
ATGGACAGTT CCTGTGGCAT AACAGTTCAA GGGCAGAAGC TAAGAAGCAG CAACATGCCA 
GTGAGTGGGA ACCAGCAAAA TTTTGGTCAT CCCATCGCAT CCAGTGATCA AGCCACCAGT 
ATGGCAAATG GGATGCAAAA CAGGAATGTG ATGGAACAGG AATATTTGCA AAACGAACTG 
GTTGGAGATG GCATTCATTA CCAGGGAGTC AATCAGTCCA GTCCAATGAC ACTAGGGCAG 
GTTAGTCCTA CCTCACAAAG CAGCCTCCAT CAGGGGCCAC AGAGTTGTCC TCCGGTATCT 
CATACCATTG GTAACCAGTC TTCAGGTTTG TCAGTGGCCA AGAGTTACCA GCCATGTGCT 
AATTACAGTG GCAACAGACG GCAAAATGTG TTGAGGAACA ACCTGGCACA ACAGCAAGGA 
CATGTAAGTG ATGGCAACCA GACGTACAGG GTAAACACCA TTAAATTGGA GATGCAAGGT 
CAATCACAGC AGTTCTGCTC TAATATGCAG AATTACTCTG GTCAGTTATA TGACCAAACC 
ACAGGCTTTA GCCACCAAGC TATGAAAATA GGTTCTTCTT TCTTTGTTTC GGAAGCTAAC 
TGCCTGCTGC AGGAGACTGC TACTGCAAAC TCCTCTGAGC TTCTTTCCCC GGGGGCTAAC 
CAAGTATCAA GCACAGTTGA CAGCCTTGAC AGCAACAGCC TAGAGGGTGT GCAGATTGAT 
TTTGATGCTA TCATAGATGA TGGAGACCAT GTCAGCTTAA TTTCAGGAGC CCTGAGCCCA 
AGCATCATTC AGAATCTCTC CCGCAATTCC TCACGCCTCA CCACTCCCCG AGCGTCTCTT 
ACCTTCCCAG CTATGCCTGT AAGCACAACC AACATGGCTA TTGGGGACAT GAGTTCTTTG 
TTGACCTCAC TTGCAGAAGA AAGCAAGTTT CTTGCTGTTA TGCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137047.1
CDS	175..4902
Translation	MEAQSHSSTTTEKKKVENSIVKCSNRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQGGQGLSKISEEPSTSSEERASLIKKEIHGSISHLPEPSVPYRGTLFTMDPRNGYMDPHYHPPHLFPAFHPPVPIDARHHEGRYHYEPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASESPFSTPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGALHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPPTPVSLQQMHQQIISRQQTLGSAFGHSPPLIHPAPTFPTQRPIPGIPSVLNPVQVSSGPSESTQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGAGSVQEQPEGMTPVKEEGDRDESKQEPEVVYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDPGSHSQTRSPGQQTQGATGEQKDLNSTTSRREECLQVKAVKSEKPMTSQPSPGGQSTCSSEQSPISNYSNNGIELTLTGGGSVGDLSVIDETPIMDSTISTATTALGLQARRNMTGTKWMEQVKLERLKQVNGMLPRLNPVPPSKAPTLPPLIGNGTQSNSSCSVGGSMTILPNRNELSSTDITVLNMLNRRDSNTSTISSAYLSSRRSSGISPCFSSRRSSDASQAEGRPQNVSVADSYDPISTDASRRSSEASQCDDLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDCRESGISPLPPVNAPRRCSDGGANGYSRRHLLSHDALGNGTRRASDPVRMVSNNLSVPRVQHFNSLNSFNSPALPPSMEKRNLVLQNCTHSEGGVFRGFSSPCPPSISENVTLEAVTMEAGGSLNDEDLLPDDVVQYLNSQNQGTCDHLLNNVLDSNKMHHSVVLGNNNPSSFDRAPPASSQPAGSEVSKSDLPIQWNEVSSGSSDLSPTKLKCSQRSAVQQARAFGLYNNMIVQQQNLQRGNVYQQNGYQNLMENNGSYSLQQNTVLGSGASSSFGMQPNKPYGESVSRQPMIFGAMDSSCGITVQGQKLRSSNMPVSGNQQNFGHPIASSDQATSMANGMQNRNVMEQEYLQNELVGDGIHYQGVNQSSPMTLGQVSPTSQSSLHQGPQSCPPVSHTIGNQSSGLSVAKSYQPCANYSGNRRQNVLRNNLAQQQGHVSDGNQTYRVNTIKLEMQGQSQQFCSNMQNYSGQLYDQTTGFSHQAMKIGSSFFVSEANCLLQETATANSSELLSPGANQVSSTVDSLDSNSLEGVQIDFDAIIDDGDHVSLISGALSPSIIQNLSRNSSRLTTPRASLTFPAMPVSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_015281561.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281561.2

ORF Insert Sequence:

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGAACAA ACCCACAAGT 
GAGTCTGCAG TGAGCAGCAC TGGGGATCCA ATGCACAACA AGCGCTCCAA GATAAAGCCG 
GATGAGGACC TCCCCAGCCC GGGTGCGGGA AGCGTGCAGG AACAGCCAGA AGGTATGACC 
CCGGTAAAGG AGGAAGGGGA CAGAGATGAA AGCAAACAGG AGCCTGAAGT GGTCTACGAG 
ACGAACTGCC ATTGGGAAGG CTGTTCCCGG GAGTTTGACA CACAGGAACA GCTAGTGCAT 
CATATAAATA ATGACCACAT ACATGGTGAG AAGAAAGAGT TTGTGTGCCG ATGGCTGGAC 
TGTTCCCGGG AGCAGAAACC ATTCAAAGCC CAGTATATGC TGGTGGTCCA CATGAGGAGA 
CATACAGGGG AAAAGCCACA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCCAGA 
CTAGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA TGTGTGCGAA 
CACGAGGGCT GCAACAAAGC TTTCTCCAAT GCATCTGACA GGGCCAAGCA CCAAAACAGG 
ACTCATTCAA ATGAAAAACC ATATGTTTGC AAGATCCCAG GCTGCACAAA GCGCTATACA 
GACCCTAGTT CTCTCCGGAA ACATGTGAAG ACTGTGCATG GCCCAGAAGC ACACGTCACC 
AAGAAGCAGC GAGGAGACAT CCACCCAAGG CCTCCACCAC CCAGAGACCC AGGCAGCCAC 
TCTCAGACAC GATCACCAGG CCAGCAGACT CAGGGTGCGA CTGGGGAGCA GAAGGACCTC 
AACAGCACTA CCTCAAGGCG TGAAGAATGC CTCCAAGTGA AAGCAGTCAA GTCAGAGAAA 
CCAATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTACAT GCAGCAGCGA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAA TGGGATCGAG CTTACTCTGA CTGGTGGCGG TAGTGTAGGA 
GACCTCAGTG TCATCGATGA AACCCCAATC ATGGACTCTA CCATTTCCAC AGCCACCACA 
GCACTTGGCT TACAGGCCAG GAGGAATATG ACAGGGACCA AATGGATGGA GCAAGTGAAA 
TTAGAAAGGT TGAAACAAGT TAATGGAATG CTTCCAAGAC TGAACCCCGT TCCACCTTCC 
AAAGCCCCGA CCTTGCCGCC TCTCATAGGA AATGGTACCC AATCAAACAG CAGCTGCAGT 
GTAGGAGGAT CCATGACTAT TCTGCCAAAC AGGAATGAAC TTTCTAGTAC AGACATCACT 
GTGTTGAACA TGCTTAACAG GAGGGACAGC AATACGAGCA CAATCAGTTC AGCCTACTTG 
AGCAGCCGCA GGTCTTCTGG AATCTCACCT TGCTTCTCCA GCCGGAGGTC CAGTGATGCC 
TCTCAGGCTG AGGGAAGACC ACAAAATGTG AGCGTTGCAG ACTCCTATGA CCCCATTTCA 
ACGGATGCCT CTCGGCGCTC AAGTGAAGCA AGCCAATGTG ATGACCTGCC AAGTCTTCTC 
AGCCTCACCC CAGCCCAGCA ATACAGGCTG AAAGCTAAAT ATGCAGCAGC TACTGGTGGA 
CCCCCACCAA CTCCACTGCC TAATATGGAG AGGATGAGCC TCAAAACAAG GATGGCACTC 
TTGGGTGACT GCAGGGAGTC TGGAATATCT CCACTGCCTC CAGTGAATGC CCCTAGAAGA 
TGTAGTGATG GTGGGGCAAA TGGTTACAGC AGGAGGCATC TTCTATCTCA TGATGCTCTA 
GGAAATGGGA CAAGGAGAGC CAGTGATCCA GTAAGAATGG TCTCCAATAA CCTCTCTGTC 
CCTAGAGTCC AACATTTCAA CAGTCTTAAT AGCTTTAACT CTCCTGCTTT GCCTCCATCC 
ATGGAAAAGC GCAACCTTGT CCTTCAGAAC TGTACCCATT CTGAGGGTGG TGTTTTCCGT 
GGCTTCAGTT CCCCCTGTCC TCCAAGCATC AGCGAGAACG TCACCCTGGA GGCTGTTACG 
ATGGAAGCAG GTGGCAGTTT GAATGATGAG GATCTCCTGC CAGATGATGT GGTTCAGTAT 
CTGAATTCCC AGAACCAGGG CACGTGCGAC CACTTGTTGA ACAATGTCCT AGACAGCAAC 
AAAATGCATC ACAGTGTGGT TTTAGGAAAC AACAATCCCA GCAGCTTTGA TCGAGCCCCT 
CCAGCAAGCA GTCAGCCTGC AGGCTCAGAG GTAAGCAAGA GTGACTTACC CATTCAGTGG 
AATGAAGTAA GCTCAGGAAG TTCTGACTTA TCTCCTACAA AACTGAAATG TAGTCAGCGC 
TCAGCAGTGC AGCAGGCTAG AGCATTTGGA CTGTATAACA ACATGATCGT TCAGCAGCAG 
AACTTGCAGA GAGGCAATGT GTACCAGCAA AATGGCTATC AGAACCTGAT GGAAAACAAT 
GGTTCCTATA GTTTGCAGCA GAACACGGTC CTTGGCAGCG GAGCCAGTAG TTCTTTCGGC 
ATGCAGCCCA ATAAGCCTTA TGGTGAAAGC GTCAGCAGGC AACCTATGAT TTTTGGGGCA 
ATGGACAGTT CCTGTGGCAT AACAGTTCAA GGGCAGAAGC TAAGAAGCAG CAACATGCCA 
GTGAGTGGGA ACCAGCAAAA TTTTGGTCAT CCCATCGCAT CCAGTGATCA AGCCACCAGT 
ATGGCAAATG GGATGCAAAA CAGGAATGTG ATGGAACAGG AATATTTGCA AAACGAACTG 
GTTGGAGATG GCATTCATTA CCAGGGAGTC AATCAGTCCA GTCCAATGAC ACTAGGGCAG 
GTTAGTCCTA CCTCACAAAG CAGCCTCCAT CAGGGGCCAC AGAGTTGTCC TCCGGTATCT 
CATACCATTG GTAACCAGTC TTCAGGTTTG TCAGTGGCCA AGAGTTACCA GCCATGTGCT 
AATTACAGTG GCAACAGACG GCAAAATGTG TTGAGGAACA ACCTGGCACA ACAGCAAGGA 
CATGTAAGTG ATGGCAACCA GACGTACAGG GTAAACACCA TTAAATTGGA GATGCAAGGT 
CAATCACAGC AGTTCTGCTC TAATATGCAG AATTACTCTG GTCAGTTATA TGACCAAACC 
ACAGGCTTTA GCCACCAAGC TATGAAAATA GGTTCTTCTT TCTTTGTTTC GGAAGCTAAC 
TGCCTGCTGC AGGAGACTGC TACTGCAAAC TCCTCTGAGC TTCTTTCCCC GGGGGCTAAC 
CAAGTATCAA GCACAGTTGA CAGCCTTGAC AGCAACAGCC TAGAGGGTGT GCAGATTGAT 
TTTGATGCTA TCATAGATGA TGGAGACCAT GTCAGCTTAA TTTCAGGAGC CCTGAGCCCA 
AGCATCATTC AGAATCTCTC CCGCAATTCC TCACGCCTCA CCACTCCCCG AGCGTCTCTT 
ACCTTCCCAG CTATGCCTGT AAGCACAACC AACATGGCTA TTGGGGACAT GAGTTCTTTG 
TTGACCTCAC TTGCAGAAGA AAGCAAGTTT CTTGCTGTTA TGCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( NM_001271903.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001258832.1 Gallus gallus GLI family zinc finger 3 (GLI3), mRNA.

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CloneID	OGa00034
Clone ID Related Accession (Same CDS sequence)	XM_015281556.1 , NM_001271903.1 , XM_015281557.1 , XM_015281557.2 , XM_015281558.1 , XM_015281556.2 , XM_015281558.2
Accession Version	NM_001271903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Gallus gallus(chicken)
Product	transcriptional activator GLI3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000034.1, AF222990.1 and AF303886.1. On Dec 13, 2012 this sequence version replaced XM_418866.3. Sequence Note: This RefSeq record was created from transcripts and genomic sequence data because no single transcript from the same breed was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF222990.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03354473 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA 
AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG 
CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG 
ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC 
GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG 
CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG 
GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG 
AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC 
AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC 
GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC 
AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT 
ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC 
ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC 
CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC 
CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG 
AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC 
CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA 
GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC 
ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG 
AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT 
TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC 
AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC 
ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC 
TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT 
GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT 
TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT 
CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT 
GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA 
CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA 
AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT 
CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT 
GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA 
TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC 
CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT 
ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG 
TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC 
AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC 
CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG 
TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG 
CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG 
CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC 
AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC 
GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG 
GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG 
CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC 
AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA 
CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG 
CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA 
TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT 
GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA 
GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA 
GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA 
ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT 
AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT 
AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT 
GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC 
CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT 
CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT 
TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258832.1
CDS	1..4731
Translation	MEAQSHSSTTTEKKKVENSIVKCSNRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQGGQGLSKISEEPSTSSEERASLIKKEIHGSISHLPEPSVPYRGTLFTMDPRNGYMDPHYHPPHLFPAFHPPVPIDARHHEGRYHYEPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASESPFSTPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGALHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPPTPVSLQQMHQQIISRQQTLGSAFGHSPPLIHPAPTFPTQRPIPGIPSVLNPVQVSSGPSESTQQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGAGSVQEQPEGMTPVKEEGDRDESKQEPEVVYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDPGSHSQTRSPGQQTQGATGEQKDLNSTTSRREECLQVKAVKSEKPMTSQPSPGGQSTCSSEQSPISNYSNNGIELTLTGGGSVGDLSVIDETPIMDSTISTATTALGLQARRNMTGTKWMEQVKLERLKQVNGMLPRLNPVPPSKAPTLPPLIGNGTQSNSSCSVGGSMTILPNRNELSSTDITVLNMLNRRDSNTSTISSAYLSSRRSSGISPCFSSRRSSDASQAEGRPQNVSVADSYDPISTDASRRSSEASQCDDLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDCRESGISPLPPVNAPRRCSDGGANGYSRRHLLSHDALGNGTRRASDPVRMVSNNLSVPRVQHFNSLNSFNSPALPPSMEKRNLVLQNCTHSEGGVFRGFSSPCPPSISENVTLEAVTMEAGGSLNDEDLLPDDVVQYLNSQNQGTCDHLLNNVLDSNKMHHSVVLGNNNPSSFDRAPPASSQPAGSEVSKSDLPIQWNEVSSGSSDLSPTKLKCSQRSAVQQARAFGLYNNMIVQQQNLQRGNVYQQNGYQNLMENNGSYSLQQNTVLGSGASSSFGMQPNKPYGESVSRQPMIFGAMDSSCGITVQGQKLRSSNMPVSGNQQNFGHPIASSDQATSMANGMQNRNVMEQEYLQNELVGDGIHYQGVNQSSPMTLGQVSPTSQSSLHQGPQSCPPVSHTIGNQSSGLSVAKSYQPCANYSGNRRQNVLRNNLAQQQGHVSDGNQTYRVNTIKLEMQGQSQQFCSNMQNYSGQLYDQTTGFSHQAMKIGSSFFVSEANCLLQETATANSSELLSPGANQVSSTVDSLDSNSLEGVQIDFDAIIDDGDHVSLISGALSPSIIQNLSRNSSRLTTPRASLTFPAMPVSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	NM_001271903.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GLI family zinc finger 3 (GLI3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271903.1

ORF Insert Sequence:

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA 
AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG 
CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG 
ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC 
GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG 
CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG 
GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG 
AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC 
AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC 
GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC 
AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT 
ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC 
ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC 
CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC 
CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG 
AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC 
CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA 
GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC 
ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG 
AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT 
TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC 
AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC 
ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC 
TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT 
GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT 
TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT 
CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT 
GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA 
CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA 
AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT 
CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT 
GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA 
TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC 
CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT 
ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG 
TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC 
AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC 
CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG 
TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG 
CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG 
CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC 
AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC 
GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG 
GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG 
CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC 
AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA 
CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG 
CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA 
TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT 
GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA 
GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA 
GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA 
ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT 
AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT 
AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT 
GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC 
CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT 
CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT 
TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_015281556.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137042.1 Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

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CloneID	OGa00034
Clone ID Related Accession (Same CDS sequence)	XM_015281556.1 , NM_001271903.1 , XM_015281557.1 , XM_015281557.2 , XM_015281558.1 , XM_015281556.2 , XM_015281558.2
Accession Version	XM_015281556.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA 
AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG 
CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG 
ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC 
GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG 
CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG 
GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG 
AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC 
AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC 
GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC 
AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT 
ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC 
ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC 
CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC 
CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG 
AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC 
CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA 
GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC 
ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG 
AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT 
TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC 
AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC 
ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC 
TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT 
GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT 
TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT 
CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT 
GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA 
CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA 
AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT 
CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT 
GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA 
TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC 
CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT 
ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG 
TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC 
AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC 
CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG 
TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG 
CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG 
CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC 
AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC 
GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG 
GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG 
CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC 
AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA 
CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG 
CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA 
TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT 
GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA 
GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA 
GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA 
ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT 
AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT 
AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT 
GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC 
CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT 
CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT 
TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137042.1
CDS	129..4859
Translation	MEAQSHSSTTTEKKKVENSIVKCSNRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQGGQGLSKISEEPSTSSEERASLIKKEIHGSISHLPEPSVPYRGTLFTMDPRNGYMDPHYHPPHLFPAFHPPVPIDARHHEGRYHYEPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASESPFSTPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGALHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPPTPVSLQQMHQQIISRQQTLGSAFGHSPPLIHPAPTFPTQRPIPGIPSVLNPVQVSSGPSESTQQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGAGSVQEQPEGMTPVKEEGDRDESKQEPEVVYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDPGSHSQTRSPGQQTQGATGEQKDLNSTTSRREECLQVKAVKSEKPMTSQPSPGGQSTCSSEQSPISNYSNNGIELTLTGGGSVGDLSVIDETPIMDSTISTATTALGLQARRNMTGTKWMEQVKLERLKQVNGMLPRLNPVPPSKAPTLPPLIGNGTQSNSSCSVGGSMTILPNRNELSSTDITVLNMLNRRDSNTSTISSAYLSSRRSSGISPCFSSRRSSDASQAEGRPQNVSVADSYDPISTDASRRSSEASQCDDLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDCRESGISPLPPVNAPRRCSDGGANGYSRRHLLSHDALGNGTRRASDPVRMVSNNLSVPRVQHFNSLNSFNSPALPPSMEKRNLVLQNCTHSEGGVFRGFSSPCPPSISENVTLEAVTMEAGGSLNDEDLLPDDVVQYLNSQNQGTCDHLLNNVLDSNKMHHSVVLGNNNPSSFDRAPPASSQPAGSEVSKSDLPIQWNEVSSGSSDLSPTKLKCSQRSAVQQARAFGLYNNMIVQQQNLQRGNVYQQNGYQNLMENNGSYSLQQNTVLGSGASSSFGMQPNKPYGESVSRQPMIFGAMDSSCGITVQGQKLRSSNMPVSGNQQNFGHPIASSDQATSMANGMQNRNVMEQEYLQNELVGDGIHYQGVNQSSPMTLGQVSPTSQSSLHQGPQSCPPVSHTIGNQSSGLSVAKSYQPCANYSGNRRQNVLRNNLAQQQGHVSDGNQTYRVNTIKLEMQGQSQQFCSNMQNYSGQLYDQTTGFSHQAMKIGSSFFVSEANCLLQETATANSSELLSPGANQVSSTVDSLDSNSLEGVQIDFDAIIDDGDHVSLISGALSPSIIQNLSRNSSRLTTPRASLTFPAMPVSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_015281556.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281556.1

ORF Insert Sequence:

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA 
AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG 
CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG 
ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC 
GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG 
CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG 
GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG 
AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC 
AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC 
GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC 
AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT 
ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC 
ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC 
CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC 
CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG 
AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC 
CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA 
GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC 
ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG 
AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT 
TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC 
AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC 
ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC 
TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT 
GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT 
TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT 
CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT 
GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA 
CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA 
AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT 
CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT 
GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA 
TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC 
CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT 
ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG 
TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC 
AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC 
CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG 
TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG 
CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG 
CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC 
AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC 
GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG 
GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG 
CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC 
AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA 
CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG 
CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA 
TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT 
GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA 
GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA 
GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA 
ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT 
AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT 
AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT 
GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC 
CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT 
CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT 
TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_015281557.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137043.1 Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

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CloneID	OGa00034
Clone ID Related Accession (Same CDS sequence)	XM_015281556.1 , NM_001271903.1 , XM_015281557.1 , XM_015281557.2 , XM_015281558.1 , XM_015281556.2 , XM_015281558.2
Accession Version	XM_015281557.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA 
AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG 
CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG 
ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC 
GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG 
CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG 
GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG 
AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC 
AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC 
GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC 
AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT 
ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC 
ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC 
CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC 
CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG 
AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC 
CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA 
GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC 
ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG 
AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT 
TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC 
AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC 
ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC 
TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT 
GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT 
TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT 
CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT 
GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA 
CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA 
AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT 
CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT 
GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA 
TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC 
CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT 
ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG 
TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC 
AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC 
CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG 
TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG 
CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG 
CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC 
AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC 
GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG 
GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG 
CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC 
AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA 
CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG 
CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA 
TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT 
GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA 
GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA 
GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA 
ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT 
AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT 
AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT 
GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC 
CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT 
CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT 
TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137043.1
CDS	124..4854
Translation	MEAQSHSSTTTEKKKVENSIVKCSNRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQGGQGLSKISEEPSTSSEERASLIKKEIHGSISHLPEPSVPYRGTLFTMDPRNGYMDPHYHPPHLFPAFHPPVPIDARHHEGRYHYEPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASESPFSTPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGALHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPPTPVSLQQMHQQIISRQQTLGSAFGHSPPLIHPAPTFPTQRPIPGIPSVLNPVQVSSGPSESTQQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGAGSVQEQPEGMTPVKEEGDRDESKQEPEVVYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDPGSHSQTRSPGQQTQGATGEQKDLNSTTSRREECLQVKAVKSEKPMTSQPSPGGQSTCSSEQSPISNYSNNGIELTLTGGGSVGDLSVIDETPIMDSTISTATTALGLQARRNMTGTKWMEQVKLERLKQVNGMLPRLNPVPPSKAPTLPPLIGNGTQSNSSCSVGGSMTILPNRNELSSTDITVLNMLNRRDSNTSTISSAYLSSRRSSGISPCFSSRRSSDASQAEGRPQNVSVADSYDPISTDASRRSSEASQCDDLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDCRESGISPLPPVNAPRRCSDGGANGYSRRHLLSHDALGNGTRRASDPVRMVSNNLSVPRVQHFNSLNSFNSPALPPSMEKRNLVLQNCTHSEGGVFRGFSSPCPPSISENVTLEAVTMEAGGSLNDEDLLPDDVVQYLNSQNQGTCDHLLNNVLDSNKMHHSVVLGNNNPSSFDRAPPASSQPAGSEVSKSDLPIQWNEVSSGSSDLSPTKLKCSQRSAVQQARAFGLYNNMIVQQQNLQRGNVYQQNGYQNLMENNGSYSLQQNTVLGSGASSSFGMQPNKPYGESVSRQPMIFGAMDSSCGITVQGQKLRSSNMPVSGNQQNFGHPIASSDQATSMANGMQNRNVMEQEYLQNELVGDGIHYQGVNQSSPMTLGQVSPTSQSSLHQGPQSCPPVSHTIGNQSSGLSVAKSYQPCANYSGNRRQNVLRNNLAQQQGHVSDGNQTYRVNTIKLEMQGQSQQFCSNMQNYSGQLYDQTTGFSHQAMKIGSSFFVSEANCLLQETATANSSELLSPGANQVSSTVDSLDSNSLEGVQIDFDAIIDDGDHVSLISGALSPSIIQNLSRNSSRLTTPRASLTFPAMPVSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_015281557.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281557.1

ORF Insert Sequence:

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA 
AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG 
CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG 
ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC 
GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG 
CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG 
GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG 
AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC 
AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC 
GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC 
AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT 
ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC 
ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC 
CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC 
CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG 
AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC 
CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA 
GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC 
ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG 
AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT 
TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC 
AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC 
ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC 
TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT 
GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT 
TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT 
CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT 
GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA 
CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA 
AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT 
CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT 
GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA 
TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC 
CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT 
ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG 
TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC 
AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC 
CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG 
TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG 
CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG 
CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC 
AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC 
GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG 
GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG 
CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC 
AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA 
CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG 
CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA 
TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT 
GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA 
GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA 
GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA 
ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT 
AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT 
AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT 
GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC 
CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT 
CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT 
TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_015281557.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137043.1 Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa00034
Clone ID Related Accession (Same CDS sequence)	XM_015281556.1 , NM_001271903.1 , XM_015281557.1 , XM_015281557.2 , XM_015281558.1 , XM_015281556.2 , XM_015281558.2
Accession Version	XM_015281557.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA 
AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG 
CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG 
ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC 
GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG 
CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG 
GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG 
AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC 
AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC 
GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC 
AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT 
ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC 
ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC 
CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC 
CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG 
AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC 
CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA 
GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC 
ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG 
AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT 
TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC 
AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC 
ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC 
TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT 
GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT 
TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT 
CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT 
GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA 
CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA 
AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT 
CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT 
GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA 
TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC 
CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT 
ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG 
TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC 
AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC 
CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG 
TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG 
CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG 
CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC 
AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC 
GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG 
GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG 
CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC 
AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA 
CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG 
CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA 
TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT 
GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA 
GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA 
GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA 
ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT 
AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT 
AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT 
GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC 
CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT 
CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT 
TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137043.1
CDS	124..4854
Translation	MEAQSHSSTTTEKKKVENSIVKCSNRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQGGQGLSKISEEPSTSSEERASLIKKEIHGSISHLPEPSVPYRGTLFTMDPRNGYMDPHYHPPHLFPAFHPPVPIDARHHEGRYHYEPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASESPFSTPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGALHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPPTPVSLQQMHQQIISRQQTLGSAFGHSPPLIHPAPTFPTQRPIPGIPSVLNPVQVSSGPSESTQQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGAGSVQEQPEGMTPVKEEGDRDESKQEPEVVYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDPGSHSQTRSPGQQTQGATGEQKDLNSTTSRREECLQVKAVKSEKPMTSQPSPGGQSTCSSEQSPISNYSNNGIELTLTGGGSVGDLSVIDETPIMDSTISTATTALGLQARRNMTGTKWMEQVKLERLKQVNGMLPRLNPVPPSKAPTLPPLIGNGTQSNSSCSVGGSMTILPNRNELSSTDITVLNMLNRRDSNTSTISSAYLSSRRSSGISPCFSSRRSSDASQAEGRPQNVSVADSYDPISTDASRRSSEASQCDDLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDCRESGISPLPPVNAPRRCSDGGANGYSRRHLLSHDALGNGTRRASDPVRMVSNNLSVPRVQHFNSLNSFNSPALPPSMEKRNLVLQNCTHSEGGVFRGFSSPCPPSISENVTLEAVTMEAGGSLNDEDLLPDDVVQYLNSQNQGTCDHLLNNVLDSNKMHHSVVLGNNNPSSFDRAPPASSQPAGSEVSKSDLPIQWNEVSSGSSDLSPTKLKCSQRSAVQQARAFGLYNNMIVQQQNLQRGNVYQQNGYQNLMENNGSYSLQQNTVLGSGASSSFGMQPNKPYGESVSRQPMIFGAMDSSCGITVQGQKLRSSNMPVSGNQQNFGHPIASSDQATSMANGMQNRNVMEQEYLQNELVGDGIHYQGVNQSSPMTLGQVSPTSQSSLHQGPQSCPPVSHTIGNQSSGLSVAKSYQPCANYSGNRRQNVLRNNLAQQQGHVSDGNQTYRVNTIKLEMQGQSQQFCSNMQNYSGQLYDQTTGFSHQAMKIGSSFFVSEANCLLQETATANSSELLSPGANQVSSTVDSLDSNSLEGVQIDFDAIIDDGDHVSLISGALSPSIIQNLSRNSSRLTTPRASLTFPAMPVSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_015281557.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281557.2

ORF Insert Sequence:

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA 
AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG 
CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG 
ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC 
GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG 
CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG 
GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG 
AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC 
AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC 
GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC 
AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT 
ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC 
ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC 
CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC 
CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG 
AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC 
CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA 
GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC 
ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG 
AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT 
TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC 
AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC 
ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC 
TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT 
GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT 
TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT 
CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT 
GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA 
CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA 
AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT 
CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT 
GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA 
TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC 
CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT 
ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG 
TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC 
AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC 
CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG 
TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG 
CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG 
CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC 
AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC 
GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG 
GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG 
CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC 
AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA 
CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG 
CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA 
TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT 
GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA 
GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA 
GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA 
ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT 
AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT 
AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT 
GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC 
CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT 
CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT 
TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_015281558.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137044.1 Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa00034
Clone ID Related Accession (Same CDS sequence)	XM_015281556.1 , NM_001271903.1 , XM_015281557.1 , XM_015281557.2 , XM_015281558.1 , XM_015281556.2 , XM_015281558.2
Accession Version	XM_015281558.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA 
AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG 
CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG 
ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC 
GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG 
CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG 
GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG 
AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC 
AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC 
GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC 
AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT 
ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC 
ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC 
CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC 
CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG 
AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC 
CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA 
GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC 
ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG 
AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT 
TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC 
AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC 
ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC 
TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT 
GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT 
TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT 
CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT 
GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA 
CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA 
AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT 
CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT 
GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA 
TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC 
CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT 
ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG 
TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC 
AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC 
CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG 
TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG 
CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG 
CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC 
AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC 
GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG 
GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG 
CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC 
AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA 
CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG 
CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA 
TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT 
GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA 
GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA 
GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA 
ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT 
AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT 
AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT 
GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC 
CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT 
CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT 
TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137044.1
CDS	128..4858
Translation	MEAQSHSSTTTEKKKVENSIVKCSNRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQGGQGLSKISEEPSTSSEERASLIKKEIHGSISHLPEPSVPYRGTLFTMDPRNGYMDPHYHPPHLFPAFHPPVPIDARHHEGRYHYEPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASESPFSTPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGALHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPPTPVSLQQMHQQIISRQQTLGSAFGHSPPLIHPAPTFPTQRPIPGIPSVLNPVQVSSGPSESTQQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGAGSVQEQPEGMTPVKEEGDRDESKQEPEVVYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDPGSHSQTRSPGQQTQGATGEQKDLNSTTSRREECLQVKAVKSEKPMTSQPSPGGQSTCSSEQSPISNYSNNGIELTLTGGGSVGDLSVIDETPIMDSTISTATTALGLQARRNMTGTKWMEQVKLERLKQVNGMLPRLNPVPPSKAPTLPPLIGNGTQSNSSCSVGGSMTILPNRNELSSTDITVLNMLNRRDSNTSTISSAYLSSRRSSGISPCFSSRRSSDASQAEGRPQNVSVADSYDPISTDASRRSSEASQCDDLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDCRESGISPLPPVNAPRRCSDGGANGYSRRHLLSHDALGNGTRRASDPVRMVSNNLSVPRVQHFNSLNSFNSPALPPSMEKRNLVLQNCTHSEGGVFRGFSSPCPPSISENVTLEAVTMEAGGSLNDEDLLPDDVVQYLNSQNQGTCDHLLNNVLDSNKMHHSVVLGNNNPSSFDRAPPASSQPAGSEVSKSDLPIQWNEVSSGSSDLSPTKLKCSQRSAVQQARAFGLYNNMIVQQQNLQRGNVYQQNGYQNLMENNGSYSLQQNTVLGSGASSSFGMQPNKPYGESVSRQPMIFGAMDSSCGITVQGQKLRSSNMPVSGNQQNFGHPIASSDQATSMANGMQNRNVMEQEYLQNELVGDGIHYQGVNQSSPMTLGQVSPTSQSSLHQGPQSCPPVSHTIGNQSSGLSVAKSYQPCANYSGNRRQNVLRNNLAQQQGHVSDGNQTYRVNTIKLEMQGQSQQFCSNMQNYSGQLYDQTTGFSHQAMKIGSSFFVSEANCLLQETATANSSELLSPGANQVSSTVDSLDSNSLEGVQIDFDAIIDDGDHVSLISGALSPSIIQNLSRNSSRLTTPRASLTFPAMPVSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_015281558.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281558.1

ORF Insert Sequence:

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA 
AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG 
CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG 
ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC 
GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG 
CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG 
GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG 
AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC 
AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC 
GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC 
AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT 
ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC 
ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC 
CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC 
CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG 
AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC 
CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA 
GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC 
ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG 
AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT 
TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC 
AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC 
ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC 
TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT 
GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT 
TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT 
CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT 
GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA 
CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA 
AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT 
CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT 
GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA 
TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC 
CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT 
ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG 
TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC 
AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC 
CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG 
TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG 
CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG 
CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC 
AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC 
GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG 
GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG 
CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC 
AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA 
CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG 
CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA 
TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT 
GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA 
GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA 
GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA 
ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT 
AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT 
AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT 
GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC 
CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT 
CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT 
TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_015281556.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137042.1 Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

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CloneID	OGa00034
Clone ID Related Accession (Same CDS sequence)	XM_015281556.1 , NM_001271903.1 , XM_015281557.1 , XM_015281557.2 , XM_015281558.1 , XM_015281556.2 , XM_015281558.2
Accession Version	XM_015281556.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281556.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA 
AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG 
CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG 
ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC 
GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG 
CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG 
GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG 
AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC 
AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC 
GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC 
AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT 
ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC 
ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC 
CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC 
CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG 
AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC 
CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA 
GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC 
ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG 
AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT 
TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC 
AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC 
ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC 
TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT 
GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT 
TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT 
CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT 
GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA 
CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA 
AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT 
CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT 
GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA 
TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC 
CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT 
ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG 
TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC 
AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC 
CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG 
TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG 
CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG 
CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC 
AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC 
GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG 
GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG 
CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC 
AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA 
CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG 
CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA 
TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT 
GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA 
GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA 
GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA 
ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT 
AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT 
AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT 
GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC 
CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT 
CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT 
TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137042.1
CDS	175..4905
Translation	MEAQSHSSTTTEKKKVENSIVKCSNRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQGGQGLSKISEEPSTSSEERASLIKKEIHGSISHLPEPSVPYRGTLFTMDPRNGYMDPHYHPPHLFPAFHPPVPIDARHHEGRYHYEPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASESPFSTPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGALHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPPTPVSLQQMHQQIISRQQTLGSAFGHSPPLIHPAPTFPTQRPIPGIPSVLNPVQVSSGPSESTQQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGAGSVQEQPEGMTPVKEEGDRDESKQEPEVVYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDPGSHSQTRSPGQQTQGATGEQKDLNSTTSRREECLQVKAVKSEKPMTSQPSPGGQSTCSSEQSPISNYSNNGIELTLTGGGSVGDLSVIDETPIMDSTISTATTALGLQARRNMTGTKWMEQVKLERLKQVNGMLPRLNPVPPSKAPTLPPLIGNGTQSNSSCSVGGSMTILPNRNELSSTDITVLNMLNRRDSNTSTISSAYLSSRRSSGISPCFSSRRSSDASQAEGRPQNVSVADSYDPISTDASRRSSEASQCDDLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDCRESGISPLPPVNAPRRCSDGGANGYSRRHLLSHDALGNGTRRASDPVRMVSNNLSVPRVQHFNSLNSFNSPALPPSMEKRNLVLQNCTHSEGGVFRGFSSPCPPSISENVTLEAVTMEAGGSLNDEDLLPDDVVQYLNSQNQGTCDHLLNNVLDSNKMHHSVVLGNNNPSSFDRAPPASSQPAGSEVSKSDLPIQWNEVSSGSSDLSPTKLKCSQRSAVQQARAFGLYNNMIVQQQNLQRGNVYQQNGYQNLMENNGSYSLQQNTVLGSGASSSFGMQPNKPYGESVSRQPMIFGAMDSSCGITVQGQKLRSSNMPVSGNQQNFGHPIASSDQATSMANGMQNRNVMEQEYLQNELVGDGIHYQGVNQSSPMTLGQVSPTSQSSLHQGPQSCPPVSHTIGNQSSGLSVAKSYQPCANYSGNRRQNVLRNNLAQQQGHVSDGNQTYRVNTIKLEMQGQSQQFCSNMQNYSGQLYDQTTGFSHQAMKIGSSFFVSEANCLLQETATANSSELLSPGANQVSSTVDSLDSNSLEGVQIDFDAIIDDGDHVSLISGALSPSIIQNLSRNSSRLTTPRASLTFPAMPVSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_015281556.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281556.2

ORF Insert Sequence:

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA 
AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG 
CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG 
ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC 
GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG 
CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG 
GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG 
AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC 
AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC 
GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC 
AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT 
ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC 
ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC 
CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC 
CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG 
AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC 
CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA 
GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC 
ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG 
AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT 
TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC 
AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC 
ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC 
TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT 
GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT 
TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT 
CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT 
GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA 
CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA 
AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT 
CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT 
GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA 
TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC 
CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT 
ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG 
TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC 
AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC 
CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG 
TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG 
CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG 
CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC 
AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC 
GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG 
GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG 
CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC 
AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA 
CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG 
CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA 
TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT 
GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA 
GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA 
GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA 
ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT 
AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT 
AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT 
GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC 
CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT 
CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT 
TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_015281558.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015137044.1 Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.

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CloneID	OGa00034
Clone ID Related Accession (Same CDS sequence)	XM_015281556.1 , NM_001271903.1 , XM_015281557.1 , XM_015281557.2 , XM_015281558.1 , XM_015281556.2 , XM_015281558.2
Accession Version	XM_015281558.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA 
AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG 
CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG 
ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC 
GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG 
CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG 
GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG 
AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC 
AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC 
GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC 
AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT 
ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC 
ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC 
CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC 
CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG 
AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC 
CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA 
GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC 
ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG 
AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT 
TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC 
AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC 
ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC 
TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT 
GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT 
TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT 
CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT 
GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA 
CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA 
AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT 
CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT 
GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA 
TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC 
CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT 
ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG 
TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC 
AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC 
CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG 
TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG 
CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG 
CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC 
AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC 
GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG 
GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG 
CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC 
AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA 
CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG 
CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA 
TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT 
GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA 
GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA 
GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA 
ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT 
AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT 
AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT 
GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC 
CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT 
CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT 
TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015137044.1
CDS	175..4905
Translation	MEAQSHSSTTTEKKKVENSIVKCSNRTDVSEKAVASSTTSNEDESPGQTYHRERRNAITMQPQGGQGLSKISEEPSTSSEERASLIKKEIHGSISHLPEPSVPYRGTLFTMDPRNGYMDPHYHPPHLFPAFHPPVPIDARHHEGRYHYEPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASESPFSTPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGALHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPPTPVSLQQMHQQIISRQQTLGSAFGHSPPLIHPAPTFPTQRPIPGIPSVLNPVQVSSGPSESTQQNKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGAGSVQEQPEGMTPVKEEGDRDESKQEPEVVYETNCHWEGCSREFDTQEQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDPGSHSQTRSPGQQTQGATGEQKDLNSTTSRREECLQVKAVKSEKPMTSQPSPGGQSTCSSEQSPISNYSNNGIELTLTGGGSVGDLSVIDETPIMDSTISTATTALGLQARRNMTGTKWMEQVKLERLKQVNGMLPRLNPVPPSKAPTLPPLIGNGTQSNSSCSVGGSMTILPNRNELSSTDITVLNMLNRRDSNTSTISSAYLSSRRSSGISPCFSSRRSSDASQAEGRPQNVSVADSYDPISTDASRRSSEASQCDDLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDCRESGISPLPPVNAPRRCSDGGANGYSRRHLLSHDALGNGTRRASDPVRMVSNNLSVPRVQHFNSLNSFNSPALPPSMEKRNLVLQNCTHSEGGVFRGFSSPCPPSISENVTLEAVTMEAGGSLNDEDLLPDDVVQYLNSQNQGTCDHLLNNVLDSNKMHHSVVLGNNNPSSFDRAPPASSQPAGSEVSKSDLPIQWNEVSSGSSDLSPTKLKCSQRSAVQQARAFGLYNNMIVQQQNLQRGNVYQQNGYQNLMENNGSYSLQQNTVLGSGASSSFGMQPNKPYGESVSRQPMIFGAMDSSCGITVQGQKLRSSNMPVSGNQQNFGHPIASSDQATSMANGMQNRNVMEQEYLQNELVGDGIHYQGVNQSSPMTLGQVSPTSQSSLHQGPQSCPPVSHTIGNQSSGLSVAKSYQPCANYSGNRRQNVLRNNLAQQQGHVSDGNQTYRVNTIKLEMQGQSQQFCSNMQNYSGQLYDQTTGFSHQAMKIGSSFFVSEANCLLQETATANSSELLSPGANQVSSTVDSLDSNSLEGVQIDFDAIIDDGDHVSLISGALSPSIIQNLSRNSSRLTTPRASLTFPAMPVSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_015281558.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281558.2

ORF Insert Sequence:

ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG 
CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG 
GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT 
TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT 
CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA 
CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT 
GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA 
AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC 
ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT 
GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG 
ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT 
GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG 
AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT 
GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT 
CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC 
GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG 
CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT 
CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC 
CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA 
AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG 
CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG 
ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC 
GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG 
CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG 
GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG 
AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC 
AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC 
GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC 
AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT 
ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC 
ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC 
CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC 
CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG 
AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC 
CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA 
GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC 
ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG 
AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT 
TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC 
AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC 
ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC 
TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT 
GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT 
TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT 
CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT 
GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA 
CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA 
AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT 
CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT 
GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA 
TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC 
CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT 
ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG 
TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC 
AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC 
CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG 
TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG 
CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG 
CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC 
AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC 
GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG 
GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG 
CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC 
AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA 
CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG 
CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA 
TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT 
GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA 
GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA 
GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA 
ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT 
AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT 
AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT 
GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC 
CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT 
CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT 
TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GLI3
Entrez Gene ID	420769
Full Name	GLI family zinc finger 3
Synonyms	GLI3FL
General protein information	Preferred Names GLI family zinc finger 3 Names transcriptional activator GLI3 GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome) GLI3 full length protein
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Homo sapiens (human)	GLI3	NP_000159.3
Rattus norvegicus (Norway rat)	Gli3	NP_536330.1
Danio rerio (zebrafish)	gli3	NP_991291.1
Pan troglodytes (chimpanzee)	GLI3	NP_001029362.1
Canis lupus familiaris (dog)	GLI3	XP_005630984.1
Bos taurus (cattle)	LOC785371	XP_005205717.1
Gallus gallus (chicken)	GLI3	NP_001258832.1
	GLI3	XP_001098108.1
Mus musculus (house mouse)	Gli3	NP_032156.2
Xenopus tropicalis (tropical clawed frog)	gli3	XP_004915408.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

GLI3 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

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