GLI3 cDNA ORF clone, Gallus gallus(chicken)

The following GLI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa31660 XM_015281561.2
Latest version!
Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa00034 NM_001271903.1
Latest version!
Gallus gallus GLI family zinc finger 3 (GLI3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa00034 XM_015281558.2
Latest version!
Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa00034 XM_015281557.2
Latest version!
Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa00034 XM_015281556.2
Latest version!
Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OGa31660 XM_015281561.1 Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa00034 XM_015281557.1 Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa00034 XM_015281556.1 Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa00034 XM_015281558.1 Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa31660
    Clone ID Related Accession (Same CDS sequence) XM_015281561.1 , XM_015281561.2
    Accession Version XM_015281561.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4728bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product transcriptional activator GLI3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGAACAA ACCCACAAGT
    GAGTCTGCAG TGAGCAGCAC TGGGGATCCA ATGCACAACA AGCGCTCCAA GATAAAGCCG
    GATGAGGACC TCCCCAGCCC GGGTGCGGGA AGCGTGCAGG AACAGCCAGA AGGTATGACC
    CCGGTAAAGG AGGAAGGGGA CAGAGATGAA AGCAAACAGG AGCCTGAAGT GGTCTACGAG
    ACGAACTGCC ATTGGGAAGG CTGTTCCCGG GAGTTTGACA CACAGGAACA GCTAGTGCAT
    CATATAAATA ATGACCACAT ACATGGTGAG AAGAAAGAGT TTGTGTGCCG ATGGCTGGAC
    TGTTCCCGGG AGCAGAAACC ATTCAAAGCC CAGTATATGC TGGTGGTCCA CATGAGGAGA
    CATACAGGGG AAAAGCCACA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCCAGA
    CTAGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA TGTGTGCGAA
    CACGAGGGCT GCAACAAAGC TTTCTCCAAT GCATCTGACA GGGCCAAGCA CCAAAACAGG
    ACTCATTCAA ATGAAAAACC ATATGTTTGC AAGATCCCAG GCTGCACAAA GCGCTATACA
    GACCCTAGTT CTCTCCGGAA ACATGTGAAG ACTGTGCATG GCCCAGAAGC ACACGTCACC
    AAGAAGCAGC GAGGAGACAT CCACCCAAGG CCTCCACCAC CCAGAGACCC AGGCAGCCAC
    TCTCAGACAC GATCACCAGG CCAGCAGACT CAGGGTGCGA CTGGGGAGCA GAAGGACCTC
    AACAGCACTA CCTCAAGGCG TGAAGAATGC CTCCAAGTGA AAGCAGTCAA GTCAGAGAAA
    CCAATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTACAT GCAGCAGCGA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAA TGGGATCGAG CTTACTCTGA CTGGTGGCGG TAGTGTAGGA
    GACCTCAGTG TCATCGATGA AACCCCAATC ATGGACTCTA CCATTTCCAC AGCCACCACA
    GCACTTGGCT TACAGGCCAG GAGGAATATG ACAGGGACCA AATGGATGGA GCAAGTGAAA
    TTAGAAAGGT TGAAACAAGT TAATGGAATG CTTCCAAGAC TGAACCCCGT TCCACCTTCC
    AAAGCCCCGA CCTTGCCGCC TCTCATAGGA AATGGTACCC AATCAAACAG CAGCTGCAGT
    GTAGGAGGAT CCATGACTAT TCTGCCAAAC AGGAATGAAC TTTCTAGTAC AGACATCACT
    GTGTTGAACA TGCTTAACAG GAGGGACAGC AATACGAGCA CAATCAGTTC AGCCTACTTG
    AGCAGCCGCA GGTCTTCTGG AATCTCACCT TGCTTCTCCA GCCGGAGGTC CAGTGATGCC
    TCTCAGGCTG AGGGAAGACC ACAAAATGTG AGCGTTGCAG ACTCCTATGA CCCCATTTCA
    ACGGATGCCT CTCGGCGCTC AAGTGAAGCA AGCCAATGTG ATGACCTGCC AAGTCTTCTC
    AGCCTCACCC CAGCCCAGCA ATACAGGCTG AAAGCTAAAT ATGCAGCAGC TACTGGTGGA
    CCCCCACCAA CTCCACTGCC TAATATGGAG AGGATGAGCC TCAAAACAAG GATGGCACTC
    TTGGGTGACT GCAGGGAGTC TGGAATATCT CCACTGCCTC CAGTGAATGC CCCTAGAAGA
    TGTAGTGATG GTGGGGCAAA TGGTTACAGC AGGAGGCATC TTCTATCTCA TGATGCTCTA
    GGAAATGGGA CAAGGAGAGC CAGTGATCCA GTAAGAATGG TCTCCAATAA CCTCTCTGTC
    CCTAGAGTCC AACATTTCAA CAGTCTTAAT AGCTTTAACT CTCCTGCTTT GCCTCCATCC
    ATGGAAAAGC GCAACCTTGT CCTTCAGAAC TGTACCCATT CTGAGGGTGG TGTTTTCCGT
    GGCTTCAGTT CCCCCTGTCC TCCAAGCATC AGCGAGAACG TCACCCTGGA GGCTGTTACG
    ATGGAAGCAG GTGGCAGTTT GAATGATGAG GATCTCCTGC CAGATGATGT GGTTCAGTAT
    CTGAATTCCC AGAACCAGGG CACGTGCGAC CACTTGTTGA ACAATGTCCT AGACAGCAAC
    AAAATGCATC ACAGTGTGGT TTTAGGAAAC AACAATCCCA GCAGCTTTGA TCGAGCCCCT
    CCAGCAAGCA GTCAGCCTGC AGGCTCAGAG GTAAGCAAGA GTGACTTACC CATTCAGTGG
    AATGAAGTAA GCTCAGGAAG TTCTGACTTA TCTCCTACAA AACTGAAATG TAGTCAGCGC
    TCAGCAGTGC AGCAGGCTAG AGCATTTGGA CTGTATAACA ACATGATCGT TCAGCAGCAG
    AACTTGCAGA GAGGCAATGT GTACCAGCAA AATGGCTATC AGAACCTGAT GGAAAACAAT
    GGTTCCTATA GTTTGCAGCA GAACACGGTC CTTGGCAGCG GAGCCAGTAG TTCTTTCGGC
    ATGCAGCCCA ATAAGCCTTA TGGTGAAAGC GTCAGCAGGC AACCTATGAT TTTTGGGGCA
    ATGGACAGTT CCTGTGGCAT AACAGTTCAA GGGCAGAAGC TAAGAAGCAG CAACATGCCA
    GTGAGTGGGA ACCAGCAAAA TTTTGGTCAT CCCATCGCAT CCAGTGATCA AGCCACCAGT
    ATGGCAAATG GGATGCAAAA CAGGAATGTG ATGGAACAGG AATATTTGCA AAACGAACTG
    GTTGGAGATG GCATTCATTA CCAGGGAGTC AATCAGTCCA GTCCAATGAC ACTAGGGCAG
    GTTAGTCCTA CCTCACAAAG CAGCCTCCAT CAGGGGCCAC AGAGTTGTCC TCCGGTATCT
    CATACCATTG GTAACCAGTC TTCAGGTTTG TCAGTGGCCA AGAGTTACCA GCCATGTGCT
    AATTACAGTG GCAACAGACG GCAAAATGTG TTGAGGAACA ACCTGGCACA ACAGCAAGGA
    CATGTAAGTG ATGGCAACCA GACGTACAGG GTAAACACCA TTAAATTGGA GATGCAAGGT
    CAATCACAGC AGTTCTGCTC TAATATGCAG AATTACTCTG GTCAGTTATA TGACCAAACC
    ACAGGCTTTA GCCACCAAGC TATGAAAATA GGTTCTTCTT TCTTTGTTTC GGAAGCTAAC
    TGCCTGCTGC AGGAGACTGC TACTGCAAAC TCCTCTGAGC TTCTTTCCCC GGGGGCTAAC
    CAAGTATCAA GCACAGTTGA CAGCCTTGAC AGCAACAGCC TAGAGGGTGT GCAGATTGAT
    TTTGATGCTA TCATAGATGA TGGAGACCAT GTCAGCTTAA TTTCAGGAGC CCTGAGCCCA
    AGCATCATTC AGAATCTCTC CCGCAATTCC TCACGCCTCA CCACTCCCCG AGCGTCTCTT
    ACCTTCCCAG CTATGCCTGT AAGCACAACC AACATGGCTA TTGGGGACAT GAGTTCTTTG
    TTGACCTCAC TTGCAGAAGA AAGCAAGTTT CTTGCTGTTA TGCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137047.1
    CDS129..4856
    Translation

    Target ORF information:

    RefSeq Version XM_015281561.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281561.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGAACAA ACCCACAAGT
    GAGTCTGCAG TGAGCAGCAC TGGGGATCCA ATGCACAACA AGCGCTCCAA GATAAAGCCG
    GATGAGGACC TCCCCAGCCC GGGTGCGGGA AGCGTGCAGG AACAGCCAGA AGGTATGACC
    CCGGTAAAGG AGGAAGGGGA CAGAGATGAA AGCAAACAGG AGCCTGAAGT GGTCTACGAG
    ACGAACTGCC ATTGGGAAGG CTGTTCCCGG GAGTTTGACA CACAGGAACA GCTAGTGCAT
    CATATAAATA ATGACCACAT ACATGGTGAG AAGAAAGAGT TTGTGTGCCG ATGGCTGGAC
    TGTTCCCGGG AGCAGAAACC ATTCAAAGCC CAGTATATGC TGGTGGTCCA CATGAGGAGA
    CATACAGGGG AAAAGCCACA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCCAGA
    CTAGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA TGTGTGCGAA
    CACGAGGGCT GCAACAAAGC TTTCTCCAAT GCATCTGACA GGGCCAAGCA CCAAAACAGG
    ACTCATTCAA ATGAAAAACC ATATGTTTGC AAGATCCCAG GCTGCACAAA GCGCTATACA
    GACCCTAGTT CTCTCCGGAA ACATGTGAAG ACTGTGCATG GCCCAGAAGC ACACGTCACC
    AAGAAGCAGC GAGGAGACAT CCACCCAAGG CCTCCACCAC CCAGAGACCC AGGCAGCCAC
    TCTCAGACAC GATCACCAGG CCAGCAGACT CAGGGTGCGA CTGGGGAGCA GAAGGACCTC
    AACAGCACTA CCTCAAGGCG TGAAGAATGC CTCCAAGTGA AAGCAGTCAA GTCAGAGAAA
    CCAATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTACAT GCAGCAGCGA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAA TGGGATCGAG CTTACTCTGA CTGGTGGCGG TAGTGTAGGA
    GACCTCAGTG TCATCGATGA AACCCCAATC ATGGACTCTA CCATTTCCAC AGCCACCACA
    GCACTTGGCT TACAGGCCAG GAGGAATATG ACAGGGACCA AATGGATGGA GCAAGTGAAA
    TTAGAAAGGT TGAAACAAGT TAATGGAATG CTTCCAAGAC TGAACCCCGT TCCACCTTCC
    AAAGCCCCGA CCTTGCCGCC TCTCATAGGA AATGGTACCC AATCAAACAG CAGCTGCAGT
    GTAGGAGGAT CCATGACTAT TCTGCCAAAC AGGAATGAAC TTTCTAGTAC AGACATCACT
    GTGTTGAACA TGCTTAACAG GAGGGACAGC AATACGAGCA CAATCAGTTC AGCCTACTTG
    AGCAGCCGCA GGTCTTCTGG AATCTCACCT TGCTTCTCCA GCCGGAGGTC CAGTGATGCC
    TCTCAGGCTG AGGGAAGACC ACAAAATGTG AGCGTTGCAG ACTCCTATGA CCCCATTTCA
    ACGGATGCCT CTCGGCGCTC AAGTGAAGCA AGCCAATGTG ATGACCTGCC AAGTCTTCTC
    AGCCTCACCC CAGCCCAGCA ATACAGGCTG AAAGCTAAAT ATGCAGCAGC TACTGGTGGA
    CCCCCACCAA CTCCACTGCC TAATATGGAG AGGATGAGCC TCAAAACAAG GATGGCACTC
    TTGGGTGACT GCAGGGAGTC TGGAATATCT CCACTGCCTC CAGTGAATGC CCCTAGAAGA
    TGTAGTGATG GTGGGGCAAA TGGTTACAGC AGGAGGCATC TTCTATCTCA TGATGCTCTA
    GGAAATGGGA CAAGGAGAGC CAGTGATCCA GTAAGAATGG TCTCCAATAA CCTCTCTGTC
    CCTAGAGTCC AACATTTCAA CAGTCTTAAT AGCTTTAACT CTCCTGCTTT GCCTCCATCC
    ATGGAAAAGC GCAACCTTGT CCTTCAGAAC TGTACCCATT CTGAGGGTGG TGTTTTCCGT
    GGCTTCAGTT CCCCCTGTCC TCCAAGCATC AGCGAGAACG TCACCCTGGA GGCTGTTACG
    ATGGAAGCAG GTGGCAGTTT GAATGATGAG GATCTCCTGC CAGATGATGT GGTTCAGTAT
    CTGAATTCCC AGAACCAGGG CACGTGCGAC CACTTGTTGA ACAATGTCCT AGACAGCAAC
    AAAATGCATC ACAGTGTGGT TTTAGGAAAC AACAATCCCA GCAGCTTTGA TCGAGCCCCT
    CCAGCAAGCA GTCAGCCTGC AGGCTCAGAG GTAAGCAAGA GTGACTTACC CATTCAGTGG
    AATGAAGTAA GCTCAGGAAG TTCTGACTTA TCTCCTACAA AACTGAAATG TAGTCAGCGC
    TCAGCAGTGC AGCAGGCTAG AGCATTTGGA CTGTATAACA ACATGATCGT TCAGCAGCAG
    AACTTGCAGA GAGGCAATGT GTACCAGCAA AATGGCTATC AGAACCTGAT GGAAAACAAT
    GGTTCCTATA GTTTGCAGCA GAACACGGTC CTTGGCAGCG GAGCCAGTAG TTCTTTCGGC
    ATGCAGCCCA ATAAGCCTTA TGGTGAAAGC GTCAGCAGGC AACCTATGAT TTTTGGGGCA
    ATGGACAGTT CCTGTGGCAT AACAGTTCAA GGGCAGAAGC TAAGAAGCAG CAACATGCCA
    GTGAGTGGGA ACCAGCAAAA TTTTGGTCAT CCCATCGCAT CCAGTGATCA AGCCACCAGT
    ATGGCAAATG GGATGCAAAA CAGGAATGTG ATGGAACAGG AATATTTGCA AAACGAACTG
    GTTGGAGATG GCATTCATTA CCAGGGAGTC AATCAGTCCA GTCCAATGAC ACTAGGGCAG
    GTTAGTCCTA CCTCACAAAG CAGCCTCCAT CAGGGGCCAC AGAGTTGTCC TCCGGTATCT
    CATACCATTG GTAACCAGTC TTCAGGTTTG TCAGTGGCCA AGAGTTACCA GCCATGTGCT
    AATTACAGTG GCAACAGACG GCAAAATGTG TTGAGGAACA ACCTGGCACA ACAGCAAGGA
    CATGTAAGTG ATGGCAACCA GACGTACAGG GTAAACACCA TTAAATTGGA GATGCAAGGT
    CAATCACAGC AGTTCTGCTC TAATATGCAG AATTACTCTG GTCAGTTATA TGACCAAACC
    ACAGGCTTTA GCCACCAAGC TATGAAAATA GGTTCTTCTT TCTTTGTTTC GGAAGCTAAC
    TGCCTGCTGC AGGAGACTGC TACTGCAAAC TCCTCTGAGC TTCTTTCCCC GGGGGCTAAC
    CAAGTATCAA GCACAGTTGA CAGCCTTGAC AGCAACAGCC TAGAGGGTGT GCAGATTGAT
    TTTGATGCTA TCATAGATGA TGGAGACCAT GTCAGCTTAA TTTCAGGAGC CCTGAGCCCA
    AGCATCATTC AGAATCTCTC CCGCAATTCC TCACGCCTCA CCACTCCCCG AGCGTCTCTT
    ACCTTCCCAG CTATGCCTGT AAGCACAACC AACATGGCTA TTGGGGACAT GAGTTCTTTG
    TTGACCTCAC TTGCAGAAGA AAGCAAGTTT CTTGCTGTTA TGCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa31660
    Clone ID Related Accession (Same CDS sequence) XM_015281561.1 , XM_015281561.2
    Accession Version XM_015281561.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4728bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product transcriptional activator GLI3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281561.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGAACAA ACCCACAAGT
    GAGTCTGCAG TGAGCAGCAC TGGGGATCCA ATGCACAACA AGCGCTCCAA GATAAAGCCG
    GATGAGGACC TCCCCAGCCC GGGTGCGGGA AGCGTGCAGG AACAGCCAGA AGGTATGACC
    CCGGTAAAGG AGGAAGGGGA CAGAGATGAA AGCAAACAGG AGCCTGAAGT GGTCTACGAG
    ACGAACTGCC ATTGGGAAGG CTGTTCCCGG GAGTTTGACA CACAGGAACA GCTAGTGCAT
    CATATAAATA ATGACCACAT ACATGGTGAG AAGAAAGAGT TTGTGTGCCG ATGGCTGGAC
    TGTTCCCGGG AGCAGAAACC ATTCAAAGCC CAGTATATGC TGGTGGTCCA CATGAGGAGA
    CATACAGGGG AAAAGCCACA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCCAGA
    CTAGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA TGTGTGCGAA
    CACGAGGGCT GCAACAAAGC TTTCTCCAAT GCATCTGACA GGGCCAAGCA CCAAAACAGG
    ACTCATTCAA ATGAAAAACC ATATGTTTGC AAGATCCCAG GCTGCACAAA GCGCTATACA
    GACCCTAGTT CTCTCCGGAA ACATGTGAAG ACTGTGCATG GCCCAGAAGC ACACGTCACC
    AAGAAGCAGC GAGGAGACAT CCACCCAAGG CCTCCACCAC CCAGAGACCC AGGCAGCCAC
    TCTCAGACAC GATCACCAGG CCAGCAGACT CAGGGTGCGA CTGGGGAGCA GAAGGACCTC
    AACAGCACTA CCTCAAGGCG TGAAGAATGC CTCCAAGTGA AAGCAGTCAA GTCAGAGAAA
    CCAATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTACAT GCAGCAGCGA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAA TGGGATCGAG CTTACTCTGA CTGGTGGCGG TAGTGTAGGA
    GACCTCAGTG TCATCGATGA AACCCCAATC ATGGACTCTA CCATTTCCAC AGCCACCACA
    GCACTTGGCT TACAGGCCAG GAGGAATATG ACAGGGACCA AATGGATGGA GCAAGTGAAA
    TTAGAAAGGT TGAAACAAGT TAATGGAATG CTTCCAAGAC TGAACCCCGT TCCACCTTCC
    AAAGCCCCGA CCTTGCCGCC TCTCATAGGA AATGGTACCC AATCAAACAG CAGCTGCAGT
    GTAGGAGGAT CCATGACTAT TCTGCCAAAC AGGAATGAAC TTTCTAGTAC AGACATCACT
    GTGTTGAACA TGCTTAACAG GAGGGACAGC AATACGAGCA CAATCAGTTC AGCCTACTTG
    AGCAGCCGCA GGTCTTCTGG AATCTCACCT TGCTTCTCCA GCCGGAGGTC CAGTGATGCC
    TCTCAGGCTG AGGGAAGACC ACAAAATGTG AGCGTTGCAG ACTCCTATGA CCCCATTTCA
    ACGGATGCCT CTCGGCGCTC AAGTGAAGCA AGCCAATGTG ATGACCTGCC AAGTCTTCTC
    AGCCTCACCC CAGCCCAGCA ATACAGGCTG AAAGCTAAAT ATGCAGCAGC TACTGGTGGA
    CCCCCACCAA CTCCACTGCC TAATATGGAG AGGATGAGCC TCAAAACAAG GATGGCACTC
    TTGGGTGACT GCAGGGAGTC TGGAATATCT CCACTGCCTC CAGTGAATGC CCCTAGAAGA
    TGTAGTGATG GTGGGGCAAA TGGTTACAGC AGGAGGCATC TTCTATCTCA TGATGCTCTA
    GGAAATGGGA CAAGGAGAGC CAGTGATCCA GTAAGAATGG TCTCCAATAA CCTCTCTGTC
    CCTAGAGTCC AACATTTCAA CAGTCTTAAT AGCTTTAACT CTCCTGCTTT GCCTCCATCC
    ATGGAAAAGC GCAACCTTGT CCTTCAGAAC TGTACCCATT CTGAGGGTGG TGTTTTCCGT
    GGCTTCAGTT CCCCCTGTCC TCCAAGCATC AGCGAGAACG TCACCCTGGA GGCTGTTACG
    ATGGAAGCAG GTGGCAGTTT GAATGATGAG GATCTCCTGC CAGATGATGT GGTTCAGTAT
    CTGAATTCCC AGAACCAGGG CACGTGCGAC CACTTGTTGA ACAATGTCCT AGACAGCAAC
    AAAATGCATC ACAGTGTGGT TTTAGGAAAC AACAATCCCA GCAGCTTTGA TCGAGCCCCT
    CCAGCAAGCA GTCAGCCTGC AGGCTCAGAG GTAAGCAAGA GTGACTTACC CATTCAGTGG
    AATGAAGTAA GCTCAGGAAG TTCTGACTTA TCTCCTACAA AACTGAAATG TAGTCAGCGC
    TCAGCAGTGC AGCAGGCTAG AGCATTTGGA CTGTATAACA ACATGATCGT TCAGCAGCAG
    AACTTGCAGA GAGGCAATGT GTACCAGCAA AATGGCTATC AGAACCTGAT GGAAAACAAT
    GGTTCCTATA GTTTGCAGCA GAACACGGTC CTTGGCAGCG GAGCCAGTAG TTCTTTCGGC
    ATGCAGCCCA ATAAGCCTTA TGGTGAAAGC GTCAGCAGGC AACCTATGAT TTTTGGGGCA
    ATGGACAGTT CCTGTGGCAT AACAGTTCAA GGGCAGAAGC TAAGAAGCAG CAACATGCCA
    GTGAGTGGGA ACCAGCAAAA TTTTGGTCAT CCCATCGCAT CCAGTGATCA AGCCACCAGT
    ATGGCAAATG GGATGCAAAA CAGGAATGTG ATGGAACAGG AATATTTGCA AAACGAACTG
    GTTGGAGATG GCATTCATTA CCAGGGAGTC AATCAGTCCA GTCCAATGAC ACTAGGGCAG
    GTTAGTCCTA CCTCACAAAG CAGCCTCCAT CAGGGGCCAC AGAGTTGTCC TCCGGTATCT
    CATACCATTG GTAACCAGTC TTCAGGTTTG TCAGTGGCCA AGAGTTACCA GCCATGTGCT
    AATTACAGTG GCAACAGACG GCAAAATGTG TTGAGGAACA ACCTGGCACA ACAGCAAGGA
    CATGTAAGTG ATGGCAACCA GACGTACAGG GTAAACACCA TTAAATTGGA GATGCAAGGT
    CAATCACAGC AGTTCTGCTC TAATATGCAG AATTACTCTG GTCAGTTATA TGACCAAACC
    ACAGGCTTTA GCCACCAAGC TATGAAAATA GGTTCTTCTT TCTTTGTTTC GGAAGCTAAC
    TGCCTGCTGC AGGAGACTGC TACTGCAAAC TCCTCTGAGC TTCTTTCCCC GGGGGCTAAC
    CAAGTATCAA GCACAGTTGA CAGCCTTGAC AGCAACAGCC TAGAGGGTGT GCAGATTGAT
    TTTGATGCTA TCATAGATGA TGGAGACCAT GTCAGCTTAA TTTCAGGAGC CCTGAGCCCA
    AGCATCATTC AGAATCTCTC CCGCAATTCC TCACGCCTCA CCACTCCCCG AGCGTCTCTT
    ACCTTCCCAG CTATGCCTGT AAGCACAACC AACATGGCTA TTGGGGACAT GAGTTCTTTG
    TTGACCTCAC TTGCAGAAGA AAGCAAGTTT CTTGCTGTTA TGCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137047.1
    CDS175..4902
    Translation

    Target ORF information:

    RefSeq Version XM_015281561.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281561.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGAACAA ACCCACAAGT
    GAGTCTGCAG TGAGCAGCAC TGGGGATCCA ATGCACAACA AGCGCTCCAA GATAAAGCCG
    GATGAGGACC TCCCCAGCCC GGGTGCGGGA AGCGTGCAGG AACAGCCAGA AGGTATGACC
    CCGGTAAAGG AGGAAGGGGA CAGAGATGAA AGCAAACAGG AGCCTGAAGT GGTCTACGAG
    ACGAACTGCC ATTGGGAAGG CTGTTCCCGG GAGTTTGACA CACAGGAACA GCTAGTGCAT
    CATATAAATA ATGACCACAT ACATGGTGAG AAGAAAGAGT TTGTGTGCCG ATGGCTGGAC
    TGTTCCCGGG AGCAGAAACC ATTCAAAGCC CAGTATATGC TGGTGGTCCA CATGAGGAGA
    CATACAGGGG AAAAGCCACA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCCAGA
    CTAGAAAACT TGAAAACACA CTTGAGATCT CACACTGGAG AGAAACCATA TGTGTGCGAA
    CACGAGGGCT GCAACAAAGC TTTCTCCAAT GCATCTGACA GGGCCAAGCA CCAAAACAGG
    ACTCATTCAA ATGAAAAACC ATATGTTTGC AAGATCCCAG GCTGCACAAA GCGCTATACA
    GACCCTAGTT CTCTCCGGAA ACATGTGAAG ACTGTGCATG GCCCAGAAGC ACACGTCACC
    AAGAAGCAGC GAGGAGACAT CCACCCAAGG CCTCCACCAC CCAGAGACCC AGGCAGCCAC
    TCTCAGACAC GATCACCAGG CCAGCAGACT CAGGGTGCGA CTGGGGAGCA GAAGGACCTC
    AACAGCACTA CCTCAAGGCG TGAAGAATGC CTCCAAGTGA AAGCAGTCAA GTCAGAGAAA
    CCAATGACAT CTCAGCCAAG CCCTGGTGGT CAGTCTACAT GCAGCAGCGA ACAGTCCCCC
    ATCAGCAACT ATTCCAACAA TGGGATCGAG CTTACTCTGA CTGGTGGCGG TAGTGTAGGA
    GACCTCAGTG TCATCGATGA AACCCCAATC ATGGACTCTA CCATTTCCAC AGCCACCACA
    GCACTTGGCT TACAGGCCAG GAGGAATATG ACAGGGACCA AATGGATGGA GCAAGTGAAA
    TTAGAAAGGT TGAAACAAGT TAATGGAATG CTTCCAAGAC TGAACCCCGT TCCACCTTCC
    AAAGCCCCGA CCTTGCCGCC TCTCATAGGA AATGGTACCC AATCAAACAG CAGCTGCAGT
    GTAGGAGGAT CCATGACTAT TCTGCCAAAC AGGAATGAAC TTTCTAGTAC AGACATCACT
    GTGTTGAACA TGCTTAACAG GAGGGACAGC AATACGAGCA CAATCAGTTC AGCCTACTTG
    AGCAGCCGCA GGTCTTCTGG AATCTCACCT TGCTTCTCCA GCCGGAGGTC CAGTGATGCC
    TCTCAGGCTG AGGGAAGACC ACAAAATGTG AGCGTTGCAG ACTCCTATGA CCCCATTTCA
    ACGGATGCCT CTCGGCGCTC AAGTGAAGCA AGCCAATGTG ATGACCTGCC AAGTCTTCTC
    AGCCTCACCC CAGCCCAGCA ATACAGGCTG AAAGCTAAAT ATGCAGCAGC TACTGGTGGA
    CCCCCACCAA CTCCACTGCC TAATATGGAG AGGATGAGCC TCAAAACAAG GATGGCACTC
    TTGGGTGACT GCAGGGAGTC TGGAATATCT CCACTGCCTC CAGTGAATGC CCCTAGAAGA
    TGTAGTGATG GTGGGGCAAA TGGTTACAGC AGGAGGCATC TTCTATCTCA TGATGCTCTA
    GGAAATGGGA CAAGGAGAGC CAGTGATCCA GTAAGAATGG TCTCCAATAA CCTCTCTGTC
    CCTAGAGTCC AACATTTCAA CAGTCTTAAT AGCTTTAACT CTCCTGCTTT GCCTCCATCC
    ATGGAAAAGC GCAACCTTGT CCTTCAGAAC TGTACCCATT CTGAGGGTGG TGTTTTCCGT
    GGCTTCAGTT CCCCCTGTCC TCCAAGCATC AGCGAGAACG TCACCCTGGA GGCTGTTACG
    ATGGAAGCAG GTGGCAGTTT GAATGATGAG GATCTCCTGC CAGATGATGT GGTTCAGTAT
    CTGAATTCCC AGAACCAGGG CACGTGCGAC CACTTGTTGA ACAATGTCCT AGACAGCAAC
    AAAATGCATC ACAGTGTGGT TTTAGGAAAC AACAATCCCA GCAGCTTTGA TCGAGCCCCT
    CCAGCAAGCA GTCAGCCTGC AGGCTCAGAG GTAAGCAAGA GTGACTTACC CATTCAGTGG
    AATGAAGTAA GCTCAGGAAG TTCTGACTTA TCTCCTACAA AACTGAAATG TAGTCAGCGC
    TCAGCAGTGC AGCAGGCTAG AGCATTTGGA CTGTATAACA ACATGATCGT TCAGCAGCAG
    AACTTGCAGA GAGGCAATGT GTACCAGCAA AATGGCTATC AGAACCTGAT GGAAAACAAT
    GGTTCCTATA GTTTGCAGCA GAACACGGTC CTTGGCAGCG GAGCCAGTAG TTCTTTCGGC
    ATGCAGCCCA ATAAGCCTTA TGGTGAAAGC GTCAGCAGGC AACCTATGAT TTTTGGGGCA
    ATGGACAGTT CCTGTGGCAT AACAGTTCAA GGGCAGAAGC TAAGAAGCAG CAACATGCCA
    GTGAGTGGGA ACCAGCAAAA TTTTGGTCAT CCCATCGCAT CCAGTGATCA AGCCACCAGT
    ATGGCAAATG GGATGCAAAA CAGGAATGTG ATGGAACAGG AATATTTGCA AAACGAACTG
    GTTGGAGATG GCATTCATTA CCAGGGAGTC AATCAGTCCA GTCCAATGAC ACTAGGGCAG
    GTTAGTCCTA CCTCACAAAG CAGCCTCCAT CAGGGGCCAC AGAGTTGTCC TCCGGTATCT
    CATACCATTG GTAACCAGTC TTCAGGTTTG TCAGTGGCCA AGAGTTACCA GCCATGTGCT
    AATTACAGTG GCAACAGACG GCAAAATGTG TTGAGGAACA ACCTGGCACA ACAGCAAGGA
    CATGTAAGTG ATGGCAACCA GACGTACAGG GTAAACACCA TTAAATTGGA GATGCAAGGT
    CAATCACAGC AGTTCTGCTC TAATATGCAG AATTACTCTG GTCAGTTATA TGACCAAACC
    ACAGGCTTTA GCCACCAAGC TATGAAAATA GGTTCTTCTT TCTTTGTTTC GGAAGCTAAC
    TGCCTGCTGC AGGAGACTGC TACTGCAAAC TCCTCTGAGC TTCTTTCCCC GGGGGCTAAC
    CAAGTATCAA GCACAGTTGA CAGCCTTGAC AGCAACAGCC TAGAGGGTGT GCAGATTGAT
    TTTGATGCTA TCATAGATGA TGGAGACCAT GTCAGCTTAA TTTCAGGAGC CCTGAGCCCA
    AGCATCATTC AGAATCTCTC CCGCAATTCC TCACGCCTCA CCACTCCCCG AGCGTCTCTT
    ACCTTCCCAG CTATGCCTGT AAGCACAACC AACATGGCTA TTGGGGACAT GAGTTCTTTG
    TTGACCTCAC TTGCAGAAGA AAGCAAGTTT CTTGCTGTTA TGCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00034
    Clone ID Related Accession (Same CDS sequence) XM_015281556.1 , NM_001271903.1 , XM_015281557.1 , XM_015281557.2 , XM_015281558.1 , XM_015281556.2 , XM_015281558.2
    Accession Version NM_001271903.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-24
    Organism Gallus gallus(chicken)
    Product transcriptional activator GLI3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000034.1, AF222990.1 and AF303886.1. On Dec 13, 2012 this sequence version replaced XM_418866.3. Sequence Note: This RefSeq record was created from transcripts and genomic sequence data because no single transcript from the same breed was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AF222990.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03354473 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA
    AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG
    CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG
    ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC
    GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG
    CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG
    GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG
    AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC
    AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC
    GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC
    AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT
    ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC
    ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC
    CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC
    CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG
    AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC
    CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA
    GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC
    ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG
    AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT
    TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC
    AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC
    ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC
    TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT
    GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT
    TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT
    CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT
    GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA
    CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA
    AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT
    CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT
    GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA
    TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC
    CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT
    ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG
    TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC
    AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC
    CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG
    TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG
    CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG
    CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC
    AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC
    GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG
    GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG
    CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC
    AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA
    CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG
    CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA
    TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT
    GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA
    GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA
    GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA
    ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT
    AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT
    AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT
    GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC
    CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT
    CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT
    TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258832.1
    CDS1..4731
    Translation

    Target ORF information:

    RefSeq Version NM_001271903.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 3 (GLI3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271903.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA
    AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG
    CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG
    ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC
    GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG
    CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG
    GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG
    AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC
    AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC
    GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC
    AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT
    ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC
    ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC
    CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC
    CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG
    AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC
    CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA
    GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC
    ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG
    AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT
    TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC
    AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC
    ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC
    TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT
    GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT
    TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT
    CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT
    GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA
    CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA
    AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT
    CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT
    GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA
    TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC
    CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT
    ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG
    TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC
    AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC
    CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG
    TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG
    CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG
    CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC
    AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC
    GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG
    GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG
    CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC
    AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA
    CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG
    CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA
    TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT
    GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA
    GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA
    GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA
    ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT
    AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT
    AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT
    GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC
    CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT
    CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT
    TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00034
    Clone ID Related Accession (Same CDS sequence) XM_015281556.1 , NM_001271903.1 , XM_015281557.1 , XM_015281557.2 , XM_015281558.1 , XM_015281556.2 , XM_015281558.2
    Accession Version XM_015281556.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product transcriptional activator GLI3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA
    AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG
    CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG
    ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC
    GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG
    CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG
    GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG
    AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC
    AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC
    GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC
    AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT
    ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC
    ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC
    CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC
    CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG
    AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC
    CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA
    GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC
    ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG
    AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT
    TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC
    AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC
    ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC
    TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT
    GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT
    TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT
    CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT
    GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA
    CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA
    AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT
    CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT
    GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA
    TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC
    CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT
    ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG
    TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC
    AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC
    CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG
    TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG
    CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG
    CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC
    AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC
    GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG
    GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG
    CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC
    AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA
    CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG
    CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA
    TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT
    GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA
    GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA
    GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA
    ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT
    AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT
    AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT
    GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC
    CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT
    CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT
    TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137042.1
    CDS129..4859
    Translation

    Target ORF information:

    RefSeq Version XM_015281556.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281556.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA
    AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG
    CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG
    ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC
    GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG
    CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG
    GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG
    AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC
    AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC
    GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC
    AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT
    ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC
    ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC
    CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC
    CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG
    AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC
    CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA
    GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC
    ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG
    AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT
    TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC
    AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC
    ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC
    TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT
    GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT
    TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT
    CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT
    GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA
    CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA
    AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT
    CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT
    GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA
    TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC
    CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT
    ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG
    TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC
    AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC
    CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG
    TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG
    CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG
    CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC
    AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC
    GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG
    GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG
    CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC
    AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA
    CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG
    CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA
    TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT
    GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA
    GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA
    GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA
    ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT
    AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT
    AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT
    GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC
    CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT
    CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT
    TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00034
    Clone ID Related Accession (Same CDS sequence) XM_015281556.1 , NM_001271903.1 , XM_015281557.1 , XM_015281557.2 , XM_015281558.1 , XM_015281556.2 , XM_015281558.2
    Accession Version XM_015281557.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product transcriptional activator GLI3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA
    AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG
    CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG
    ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC
    GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG
    CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG
    GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG
    AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC
    AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC
    GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC
    AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT
    ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC
    ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC
    CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC
    CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG
    AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC
    CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA
    GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC
    ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG
    AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT
    TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC
    AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC
    ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC
    TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT
    GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT
    TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT
    CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT
    GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA
    CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA
    AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT
    CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT
    GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA
    TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC
    CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT
    ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG
    TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC
    AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC
    CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG
    TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG
    CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG
    CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC
    AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC
    GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG
    GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG
    CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC
    AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA
    CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG
    CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA
    TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT
    GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA
    GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA
    GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA
    ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT
    AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT
    AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT
    GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC
    CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT
    CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT
    TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137043.1
    CDS124..4854
    Translation

    Target ORF information:

    RefSeq Version XM_015281557.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281557.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA
    AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG
    CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG
    ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC
    GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG
    CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG
    GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG
    AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC
    AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC
    GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC
    AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT
    ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC
    ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC
    CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC
    CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG
    AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC
    CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA
    GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC
    ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG
    AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT
    TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC
    AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC
    ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC
    TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT
    GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT
    TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT
    CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT
    GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA
    CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA
    AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT
    CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT
    GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA
    TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC
    CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT
    ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG
    TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC
    AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC
    CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG
    TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG
    CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG
    CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC
    AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC
    GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG
    GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG
    CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC
    AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA
    CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG
    CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA
    TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT
    GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA
    GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA
    GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA
    ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT
    AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT
    AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT
    GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC
    CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT
    CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT
    TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00034
    Clone ID Related Accession (Same CDS sequence) XM_015281556.1 , NM_001271903.1 , XM_015281557.1 , XM_015281557.2 , XM_015281558.1 , XM_015281556.2 , XM_015281558.2
    Accession Version XM_015281557.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product transcriptional activator GLI3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA
    AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG
    CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG
    ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC
    GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG
    CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG
    GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG
    AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC
    AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC
    GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC
    AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT
    ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC
    ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC
    CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC
    CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG
    AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC
    CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA
    GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC
    ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG
    AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT
    TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC
    AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC
    ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC
    TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT
    GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT
    TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT
    CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT
    GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA
    CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA
    AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT
    CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT
    GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA
    TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC
    CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT
    ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG
    TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC
    AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC
    CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG
    TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG
    CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG
    CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC
    AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC
    GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG
    GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG
    CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC
    AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA
    CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG
    CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA
    TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT
    GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA
    GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA
    GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA
    ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT
    AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT
    AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT
    GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC
    CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT
    CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT
    TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137043.1
    CDS124..4854
    Translation

    Target ORF information:

    RefSeq Version XM_015281557.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281557.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA
    AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG
    CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG
    ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC
    GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG
    CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG
    GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG
    AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC
    AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC
    GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC
    AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT
    ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC
    ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC
    CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC
    CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG
    AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC
    CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA
    GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC
    ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG
    AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT
    TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC
    AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC
    ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC
    TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT
    GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT
    TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT
    CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT
    GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA
    CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA
    AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT
    CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT
    GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA
    TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC
    CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT
    ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG
    TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC
    AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC
    CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG
    TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG
    CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG
    CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC
    AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC
    GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG
    GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG
    CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC
    AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA
    CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG
    CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA
    TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT
    GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA
    GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA
    GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA
    ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT
    AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT
    AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT
    GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC
    CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT
    CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT
    TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00034
    Clone ID Related Accession (Same CDS sequence) XM_015281556.1 , NM_001271903.1 , XM_015281557.1 , XM_015281557.2 , XM_015281558.1 , XM_015281556.2 , XM_015281558.2
    Accession Version XM_015281558.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product transcriptional activator GLI3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA
    AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG
    CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG
    ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC
    GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG
    CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG
    GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG
    AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC
    AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC
    GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC
    AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT
    ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC
    ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC
    CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC
    CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG
    AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC
    CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA
    GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC
    ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG
    AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT
    TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC
    AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC
    ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC
    TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT
    GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT
    TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT
    CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT
    GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA
    CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA
    AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT
    CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT
    GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA
    TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC
    CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT
    ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG
    TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC
    AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC
    CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG
    TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG
    CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG
    CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC
    AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC
    GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG
    GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG
    CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC
    AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA
    CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG
    CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA
    TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT
    GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA
    GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA
    GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA
    ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT
    AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT
    AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT
    GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC
    CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT
    CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT
    TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137044.1
    CDS128..4858
    Translation

    Target ORF information:

    RefSeq Version XM_015281558.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281558.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA
    AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG
    CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG
    ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC
    GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG
    CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG
    GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG
    AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC
    AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC
    GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC
    AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT
    ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC
    ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC
    CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC
    CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG
    AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC
    CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA
    GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC
    ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG
    AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT
    TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC
    AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC
    ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC
    TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT
    GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT
    TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT
    CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT
    GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA
    CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA
    AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT
    CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT
    GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA
    TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC
    CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT
    ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG
    TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC
    AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC
    CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG
    TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG
    CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG
    CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC
    AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC
    GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG
    GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG
    CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC
    AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA
    CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG
    CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA
    TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT
    GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA
    GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA
    GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA
    ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT
    AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT
    AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT
    GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC
    CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT
    CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT
    TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00034
    Clone ID Related Accession (Same CDS sequence) XM_015281556.1 , NM_001271903.1 , XM_015281557.1 , XM_015281557.2 , XM_015281558.1 , XM_015281556.2 , XM_015281558.2
    Accession Version XM_015281556.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product transcriptional activator GLI3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281556.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA
    AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG
    CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG
    ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC
    GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG
    CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG
    GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG
    AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC
    AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC
    GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC
    AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT
    ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC
    ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC
    CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC
    CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG
    AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC
    CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA
    GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC
    ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG
    AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT
    TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC
    AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC
    ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC
    TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT
    GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT
    TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT
    CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT
    GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA
    CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA
    AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT
    CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT
    GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA
    TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC
    CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT
    ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG
    TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC
    AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC
    CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG
    TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG
    CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG
    CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC
    AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC
    GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG
    GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG
    CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC
    AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA
    CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG
    CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA
    TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT
    GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA
    GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA
    GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA
    ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT
    AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT
    AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT
    GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC
    CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT
    CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT
    TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137042.1
    CDS175..4905
    Translation

    Target ORF information:

    RefSeq Version XM_015281556.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281556.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA
    AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG
    CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG
    ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC
    GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG
    CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG
    GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG
    AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC
    AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC
    GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC
    AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT
    ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC
    ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC
    CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC
    CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG
    AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC
    CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA
    GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC
    ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG
    AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT
    TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC
    AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC
    ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC
    TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT
    GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT
    TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT
    CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT
    GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA
    CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA
    AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT
    CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT
    GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA
    TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC
    CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT
    ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG
    TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC
    AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC
    CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG
    TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG
    CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG
    CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC
    AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC
    GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG
    GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG
    CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC
    AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA
    CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG
    CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA
    TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT
    GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA
    GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA
    GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA
    ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT
    AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT
    AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT
    GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC
    CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT
    CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT
    TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00034
    Clone ID Related Accession (Same CDS sequence) XM_015281556.1 , NM_001271903.1 , XM_015281557.1 , XM_015281557.2 , XM_015281558.1 , XM_015281556.2 , XM_015281558.2
    Accession Version XM_015281558.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product transcriptional activator GLI3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA
    AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG
    CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG
    ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC
    GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG
    CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG
    GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG
    AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC
    AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC
    GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC
    AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT
    ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC
    ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC
    CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC
    CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG
    AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC
    CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA
    GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC
    ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG
    AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT
    TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC
    AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC
    ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC
    TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT
    GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT
    TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT
    CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT
    GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA
    CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA
    AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT
    CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT
    GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA
    TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC
    CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT
    ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG
    TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC
    AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC
    CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG
    TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG
    CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG
    CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC
    AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC
    GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG
    GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG
    CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC
    AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA
    CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG
    CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA
    TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT
    GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA
    GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA
    GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA
    ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT
    AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT
    AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT
    GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC
    CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT
    CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT
    TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137044.1
    CDS175..4905
    Translation

    Target ORF information:

    RefSeq Version XM_015281558.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281558.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGAGGCGC AGTCCCATAG CTCTACCACG ACAGAGAAGA AAAAAGTGGA GAATTCCATT 
    GTGAAATGCT CCAATCGAAC AGATGTCAGT GAGAAAGCTG TTGCCTCCAG CACAACTTCC
    AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGGAACGC AATCACAATG
    CAGCCACAAG GTGGGCAAGG CCTCAGCAAG ATCAGTGAGG AGCCTTCAAC ATCTAGCGAG
    GAAAGGGCCT CATTAATAAA GAAAGAGATC CATGGATCTA TATCGCACCT TCCTGAACCT
    TCTGTACCTT ATCGTGGGAC ATTGTTTACT ATGGACCCCA GAAATGGTTA CATGGATCCT
    CATTACCATC CACCTCACCT CTTTCCAGCG TTCCACCCTC CTGTACCAAT TGATGCGCGA
    CATCATGAGG GACGCTACCA TTATGAACCA TCTCCCATTC CTCCTTTGCA TGTGCCTTCT
    GCCTTATCTA GTAGCCCAAC ATATTCAGAT CTTCCATTCA TTAGAATTTC CCCACACAGA
    AATCCTGCTG CAGCATCAGA GTCTCCCTTC AGCACTCCTC ACCCGTACAT TAACCCATAC
    ATGGACTATA TCCGCTCCTT GCACAGCAGC CCATCCCTGT CCATGATCTC AGCAGCCCGT
    GGACTCAGCC CAACAGACGC TCCCCATGCT GGCGTCAGTC CAGCTGAATA TTATCATCAG
    ATGGCTCTGT TGGCAGGCCA GCGCAGCCCT TACGCAGACA TCATTCCTTC AGCTGCCACT
    GCTGGAGCTG GTGCTCTTCA TATGGAATAT CTTCACGCCA TGGATAGCAC CAGGTTTCCG
    AGTCCAAGAT TATCAGCTAG ACCAAGCCGA AAGCGTACGT TGTCTATATC CCCCCTATCT
    GATCACAGCT TTGACCTTCA GACCATGATA CGGACATCTC CAAATTCCTT GGTCACAATT
    CTCAATAATT CTCGTAGCAG TTCGTCAGCC AGTGGTTCTT ATGGCCACTT ATCTGCAAGC
    GCAATAAGCC CTGCTCTGAG TTTCACATAC CCTCCTACAC CGGTATCCCT CCAGCAAATG
    CATCAGCAAA TTATAAGTCG TCAGCAAACC CTGGGTTCTG CCTTTGGACA CAGCCCTCCT
    CTCATCCATC CTGCTCCAAC TTTTCCTACC CAGAGACCTA TCCCTGGCAT CCCCAGTGTC
    CTGAACCCTG TCCAGGTCAG CTCTGGACCT TCTGAGTCCA CACAGCAGAA CAAACCCACA
    AGTGAGTCTG CAGTGAGCAG CACTGGGGAT CCAATGCACA ACAAGCGCTC CAAGATAAAG
    CCGGATGAGG ACCTCCCCAG CCCGGGTGCG GGAAGCGTGC AGGAACAGCC AGAAGGTATG
    ACCCCGGTAA AGGAGGAAGG GGACAGAGAT GAAAGCAAAC AGGAGCCTGA AGTGGTCTAC
    GAGACGAACT GCCATTGGGA AGGCTGTTCC CGGGAGTTTG ACACACAGGA ACAGCTAGTG
    CATCATATAA ATAATGACCA CATACATGGT GAGAAGAAAG AGTTTGTGTG CCGATGGCTG
    GACTGTTCCC GGGAGCAGAA ACCATTCAAA GCCCAGTATA TGCTGGTGGT CCACATGAGG
    AGACATACAG GGGAAAAGCC ACACAAATGC ACTTTTGAAG GTTGTACAAA GGCCTACTCC
    AGACTAGAAA ACTTGAAAAC ACACTTGAGA TCTCACACTG GAGAGAAACC ATATGTGTGC
    GAACACGAGG GCTGCAACAA AGCTTTCTCC AATGCATCTG ACAGGGCCAA GCACCAAAAC
    AGGACTCATT CAAATGAAAA ACCATATGTT TGCAAGATCC CAGGCTGCAC AAAGCGCTAT
    ACAGACCCTA GTTCTCTCCG GAAACATGTG AAGACTGTGC ATGGCCCAGA AGCACACGTC
    ACCAAGAAGC AGCGAGGAGA CATCCACCCA AGGCCTCCAC CACCCAGAGA CCCAGGCAGC
    CACTCTCAGA CACGATCACC AGGCCAGCAG ACTCAGGGTG CGACTGGGGA GCAGAAGGAC
    CTCAACAGCA CTACCTCAAG GCGTGAAGAA TGCCTCCAAG TGAAAGCAGT CAAGTCAGAG
    AAACCAATGA CATCTCAGCC AAGCCCTGGT GGTCAGTCTA CATGCAGCAG CGAACAGTCC
    CCCATCAGCA ACTATTCCAA CAATGGGATC GAGCTTACTC TGACTGGTGG CGGTAGTGTA
    GGAGACCTCA GTGTCATCGA TGAAACCCCA ATCATGGACT CTACCATTTC CACAGCCACC
    ACAGCACTTG GCTTACAGGC CAGGAGGAAT ATGACAGGGA CCAAATGGAT GGAGCAAGTG
    AAATTAGAAA GGTTGAAACA AGTTAATGGA ATGCTTCCAA GACTGAACCC CGTTCCACCT
    TCCAAAGCCC CGACCTTGCC GCCTCTCATA GGAAATGGTA CCCAATCAAA CAGCAGCTGC
    AGTGTAGGAG GATCCATGAC TATTCTGCCA AACAGGAATG AACTTTCTAG TACAGACATC
    ACTGTGTTGA ACATGCTTAA CAGGAGGGAC AGCAATACGA GCACAATCAG TTCAGCCTAC
    TTGAGCAGCC GCAGGTCTTC TGGAATCTCA CCTTGCTTCT CCAGCCGGAG GTCCAGTGAT
    GCCTCTCAGG CTGAGGGAAG ACCACAAAAT GTGAGCGTTG CAGACTCCTA TGACCCCATT
    TCAACGGATG CCTCTCGGCG CTCAAGTGAA GCAAGCCAAT GTGATGACCT GCCAAGTCTT
    CTCAGCCTCA CCCCAGCCCA GCAATACAGG CTGAAAGCTA AATATGCAGC AGCTACTGGT
    GGACCCCCAC CAACTCCACT GCCTAATATG GAGAGGATGA GCCTCAAAAC AAGGATGGCA
    CTCTTGGGTG ACTGCAGGGA GTCTGGAATA TCTCCACTGC CTCCAGTGAA TGCCCCTAGA
    AGATGTAGTG ATGGTGGGGC AAATGGTTAC AGCAGGAGGC ATCTTCTATC TCATGATGCT
    CTAGGAAATG GGACAAGGAG AGCCAGTGAT CCAGTAAGAA TGGTCTCCAA TAACCTCTCT
    GTCCCTAGAG TCCAACATTT CAACAGTCTT AATAGCTTTA ACTCTCCTGC TTTGCCTCCA
    TCCATGGAAA AGCGCAACCT TGTCCTTCAG AACTGTACCC ATTCTGAGGG TGGTGTTTTC
    CGTGGCTTCA GTTCCCCCTG TCCTCCAAGC ATCAGCGAGA ACGTCACCCT GGAGGCTGTT
    ACGATGGAAG CAGGTGGCAG TTTGAATGAT GAGGATCTCC TGCCAGATGA TGTGGTTCAG
    TATCTGAATT CCCAGAACCA GGGCACGTGC GACCACTTGT TGAACAATGT CCTAGACAGC
    AACAAAATGC ATCACAGTGT GGTTTTAGGA AACAACAATC CCAGCAGCTT TGATCGAGCC
    CCTCCAGCAA GCAGTCAGCC TGCAGGCTCA GAGGTAAGCA AGAGTGACTT ACCCATTCAG
    TGGAATGAAG TAAGCTCAGG AAGTTCTGAC TTATCTCCTA CAAAACTGAA ATGTAGTCAG
    CGCTCAGCAG TGCAGCAGGC TAGAGCATTT GGACTGTATA ACAACATGAT CGTTCAGCAG
    CAGAACTTGC AGAGAGGCAA TGTGTACCAG CAAAATGGCT ATCAGAACCT GATGGAAAAC
    AATGGTTCCT ATAGTTTGCA GCAGAACACG GTCCTTGGCA GCGGAGCCAG TAGTTCTTTC
    GGCATGCAGC CCAATAAGCC TTATGGTGAA AGCGTCAGCA GGCAACCTAT GATTTTTGGG
    GCAATGGACA GTTCCTGTGG CATAACAGTT CAAGGGCAGA AGCTAAGAAG CAGCAACATG
    CCAGTGAGTG GGAACCAGCA AAATTTTGGT CATCCCATCG CATCCAGTGA TCAAGCCACC
    AGTATGGCAA ATGGGATGCA AAACAGGAAT GTGATGGAAC AGGAATATTT GCAAAACGAA
    CTGGTTGGAG ATGGCATTCA TTACCAGGGA GTCAATCAGT CCAGTCCAAT GACACTAGGG
    CAGGTTAGTC CTACCTCACA AAGCAGCCTC CATCAGGGGC CACAGAGTTG TCCTCCGGTA
    TCTCATACCA TTGGTAACCA GTCTTCAGGT TTGTCAGTGG CCAAGAGTTA CCAGCCATGT
    GCTAATTACA GTGGCAACAG ACGGCAAAAT GTGTTGAGGA ACAACCTGGC ACAACAGCAA
    GGACATGTAA GTGATGGCAA CCAGACGTAC AGGGTAAACA CCATTAAATT GGAGATGCAA
    GGTCAATCAC AGCAGTTCTG CTCTAATATG CAGAATTACT CTGGTCAGTT ATATGACCAA
    ACCACAGGCT TTAGCCACCA AGCTATGAAA ATAGGTTCTT CTTTCTTTGT TTCGGAAGCT
    AACTGCCTGC TGCAGGAGAC TGCTACTGCA AACTCCTCTG AGCTTCTTTC CCCGGGGGCT
    AACCAAGTAT CAAGCACAGT TGACAGCCTT GACAGCAACA GCCTAGAGGG TGTGCAGATT
    GATTTTGATG CTATCATAGA TGATGGAGAC CATGTCAGCT TAATTTCAGG AGCCCTGAGC
    CCAAGCATCA TTCAGAATCT CTCCCGCAAT TCCTCACGCC TCACCACTCC CCGAGCGTCT
    CTTACCTTCC CAGCTATGCC TGTAAGCACA ACCAACATGG CTATTGGGGA CATGAGTTCT
    TTGTTGACCT CACTTGCAGA AGAAAGCAAG TTTCTTGCTG TTATGCAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.