GLI3 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	GLI3
Entrez Gene ID	483244
Full Name	GLI family zinc finger 3
General protein information	Preferred Names GLI family zinc finger 3 Names zinc finger protein GLI3 transcriptional activator GLI3 GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome)
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022405177.1	XP_022260885.1	transcriptional activator GLI3 isoform X1
XM_540363.5	XP_540363.2	transcriptional activator GLI3 isoform X3
XM_540363.4	XP_540363.2	transcriptional activator GLI3 isoform X2
XM_022405178.1	XP_022260886.1	transcriptional activator GLI3 isoform X2
XM_014120825.2	XP_013976300.1	transcriptional activator GLI3 isoform X4
XM_014120824.1	XP_013976299.1	transcriptional activator GLI3 isoform X1
XM_014120825.1	XP_013976300.1	transcriptional activator GLI3 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	GLI3	NP_000159.3
Rattus norvegicus (Norway rat)	Gli3	NP_536330.1
Danio rerio (zebrafish)	gli3	NP_991291.1
Pan troglodytes (chimpanzee)	GLI3	NP_001029362.1
Canis lupus familiaris (dog)	GLI3	XP_005630984.1
Bos taurus (cattle)	LOC785371	XP_005205717.1
Gallus gallus (chicken)	GLI3	NP_001258832.1
	GLI3	XP_001098108.1
Mus musculus (house mouse)	Gli3	NP_032156.2
Xenopus tropicalis (tropical clawed frog)	gli3	XP_004915408.1

Gene Ontology

The following GLI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe19652	XM_540363.5 Latest version!	Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe294660	XM_022405177.1 Latest version!	Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe294661	XM_022405178.1 Latest version!	Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe41022	XM_014120824.1 Latest version!	Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe41023	XM_014120825.2 Latest version!	Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
OCe19652	XM_540363.4	Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe41023	XM_014120825.1	Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GLI3 ( XM_540363.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_540363.2 Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe19652
Clone ID Related Accession (Same CDS sequence)	XM_540363.5 , XM_540363.4
Accession Version	XM_540363.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transcriptional activator GLI3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_540363.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC AGAACCACAG CTCCACGACC ACTGAGAAGA AGAAAGTGGA CACTTCCATA 
GTGAAATGCT CCTCGAGAAC AGATGTCAGC GAGAAAGCGG TGGCCTCCAG CACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG 
CAGCCACAGA GCGTCCAGGG GCTCGGCAAG ATCAGCGAGG AGCCTTCGAC GTCTAGCGAG 
GAGAGGGCCT CGCTGATCAA GAAGGAGATC CATGGGTCCC TGCCGCACCT GGCCGAGCCC 
TCGGTGCCGT ACCGTGGGGC CGTGTTTGCC ATGGACCCCC GGAACGGTTA CATGGAGCCC 
CACTACCACC CTTCTCATCT CTTTCCTGCA TTCCACCCTC CTGTACCAAT CGATGCAAGA 
CATCATGAGG GGCGTTACCA TTATGACCCG TCTCCGATTC CTCCATTGCA TGTGCCTTCT 
GCTTTATCCA GTAGCCCAAC GTATTCGGAC CTGCCCTTCA TCAGGATCTC CCCACACCGG 
AACCCCGCTG CAGCCTCTGA ACCCCCCTTC AGCCCTCCAC ACCCCTACAT CAACCCCTAC 
ATGGACTACA TCCGGTCCCT ACACAGCCCG TCCCTCTCCA TGATCTCGGC AGCTCGGGGG 
CTCAGCCCTA CGGATGCCCC CCATGCTGGG GTCAGCCCAG CGGAATACTA TCATCAGATG 
GCTCTGCTGG CTGGCCAGCG CAGCCCCTAC GCAGACATCA TTCCCTCCGC TGCCACTGCC 
GGCGCTGGGG CCATCCACAT GGAATATCTT CATGCCATGG ACAGCACCAG ATTCCCCAGC 
CCCAGGCTGT CGGCCCGGCC GAGCCGAAAA CGTACACTGT CCATATCACC GCTGTCCGAT 
CACAGCTTTG ACCTTCAGAC CATGATAAGG ACATCTCCCA ACTCCTTGGT CACGATTCTC 
AATAATTCCC GCAGCAGCTC TTCAGCGAGC GGCTCCTACG GCCACTTGTC TGCGAGTGCA 
ATCAGCCCTG CCCTGAGCTT CACCTACCCT TCTGCACCCG TGTCTCTCCA CATGCATCAG 
CAAATCTTAA GCCGACAACA GAGCCTAGGC TCGGCCTTTG GACACAGCCC TCCGCTCATC 
CACCCTGCTC CAACTTTTCC AACGCAGAGG CCTATTCCTG GGATCCCTAC GGTGCTCAAC 
CCCGTCCAGG TCACTTCTGG CCCTTCTGAG TCCTCACAGA ACAAGCCGAC GAGTGAGTCC 
GCGGTGAGTA GCACTGGTGA CCTGATGCAC AACAAGCGGT CCAAGATCAA ACCTGACGAA 
GACCTACCCA GCCCGGGGCC ACGGGGCCAG CAGGAACAGC CAGAAGGAAC AACCCTGGTA 
AAGGAAGAGG GTGACAGAGA CGAAAGCAAA CAAGAGCCCG AAGTCATCTA TGAGACCAAC 
TGCCACTGGG AAGGTTGCAC GCGGGAGTTT GACACCCAGG AGCAGCTGGT GCACCATATA 
AATAATGACC ATATTCACGG CGAGAAGAAG GAGTTCGTGT GCCGGTGGAT GGATTGCTCG 
AGGGAGCAGA AGCCCTTCAA AGCCCAGTAC ATGCTGGTGG TGCACATGAG GAGACACACC 
GGGGAGAAAC CTCACAAATG CACTTTTGAA GGTTGTACAA AGGCCTACTC GAGACTAGAA 
AACTTGAAAA CGCACTTGAG ATCTCACACT GGAGAGAAAC CGTACGTCTG TGAGCACGAA 
GGTTGCAACA AGGCTTTCTC AAATGCCTCT GATCGCGCCA AGCACCAAAA CAGGACGCAT 
TCCAATGAGA AACCCTATGT GTGCAAAATC CCAGGCTGCA CCAAGCGCTA CACAGACCCA 
AGTTCCCTCC GGAAACATGT GAAGACCGTG CATGGCCCAG AGGCTCATGT CACCAAGAAG 
CAGCGTGGGG ACATACATCC TCGGCCCCCC CCACCCCGAG ATTCGGGCAG CCATTCACAG 
TCCAGGTCGC CTGGCCGGCA GACTCAGGGA GCCCTTGGGG AGCAGAAGGA CCTCAGCAAC 
ACTACCTCAA AGCGGGAAGA ATGCCTCCAA GTGAAAGCTG TCAAGGCCGA GAAGCCCATG 
ACATCTCAGC CAAGCCCTGG TGGTGAGTCT TCATGCAGCA GCCAACAGTC CCCCATCAGC 
AACTATTCCA ACAGTGGGCT CGAGCTTCCT CTGACCGACG GAGGTGGCGT GGGAGACCTC 
GGGACCATCG ACGAAACCCC AATCATGGAC TCGACCATTT CCACCGCAAC CACCGCCCTC 
GCCTTGCAAG CCAGGAGGAA CCCGGCAGGG GCCAAATGGA TGGAGCATGT GAAACTAGAA 
AGGCTCAAAC AAGTGAATGG AATGCTTCCA CGACTGAACC CCGTTCTTCC CCCCAAAGCC 
CCTGCAGTCT CTCCTCTCAT AGGAAACGGC ACTCAGCCCA GCACCAGCTG CAGTTTGGGC 
GGGCCCATAA CCCTCCTCCC GAACAGGAGC GACCTCTCCG GGGTGGACAT CACCATGCTG 
AACATGCTCA ACCGGAGGGA CAGCAGCGCC AGCACCATCA GCTCCGCCTA CCTGAGCAGC 
CGCCGCTCCT CGGGCATCTC CCCCTGCTTT TCCAGCCGCC GGTCCAGCGA GGCGTCCCAG 
GCCGAGGGCC GGCCCCAGAA CGTGAGCGTG GCCGACTCCT ACGACCCCAT CTCCACCGAC 
GCGTCGCGCA GGTCCAGCGA GGCGAGCCAG GGCGACGGGC TGCCCAGCCT GCTCAGCCTC 
ACGCCGGCGC AGCAGTACCG CCTGAAGGCC AAGTACGCGG CGGCCACGGG CGGGCCGCCC 
CCCACCCCGC TGCCCAACAT GGAGAGGATG AGCCTCAAGA CCAGGATGGC CCTGCTCGGG 
GACGCCCGGG AGCCCGCGGG GGCCCTGCCG CCCGTGCCCC CTCCGCGGCG GTGCAGCGAC 
GGCGGCGCCC ACGGCTACGG CCGCCGCCCC CTGCTGCCCC TGGACGCGCT GGGCAACGGC 
GTGCGCAGGG CCAGCGACCC GGTGCGGACG GCGTCCGACG GCCTCTCCCT GCCCCGCGTG 
CAGCGCTTCG GCAGCCTTCA CAGCTTCAAC CCGCCCGGGC CGCCTCCCAC CCTGGAGAAG 
CGGCACCTGG TGCTCCAGAA TTACACGCGG CCCGACGGCG GCCAGCCCCG CCACTTCCCG 
CCGTCCCCCT GTCCGCCGAG CATCACCGAG AACGTCACCC TGGAGGCCCT GACCATGGAC 
GCCGACGTGA GCCTCACCGA TGAGGACTTC CTGCCCGACG ACGTGGTGCA GTATCTGAAT 
TCCCAGAACC CGGCCGGGTT CGAGCAGCCC TTCCAGAGCG CCGTCTCCGA GGACAGCAAA 
GCGCCCCACG GGCCCGGCGA CTTCGACGGC CCGGGGCTGC CGGACGGTCA CGCGGGCCAC 
CCAGGCCACC CGGGCCACCC GGGCCCCGCC GGCCAGCCGT ACCGCCCGCT GGAGCAGCCG 
TGCCCCGAAG CCAGCAAAGC CGACCTGCCC ATTCAGTGGA ACGAAGTGAG CTCGGGCAGC 
GCCGACCTGT GCTCCTCCAA GCTCAAGTGT GGCCAGCGGC CGGCGGCGCA GCAGGCCCGG 
GCCTTCGGCT TCTACGGCAA CGTGGGCGCC CCCCCGCAGC CCGCCTTGCG GAGCGGGCCC 
GGCCCCGCCG GCGCCTATGC CCCCCTGGCC GAGCCCGGCC CGGCCTACGG CGGCCCCGAG 
CCCTTGGCGG GCCACGGCGC TGGAGCGGGC AGCCCCGGAG ACGCGTTCCA CGAACAGCCC 
TGCAAGGCCC CGCAGTTCGG GAGCTGCCTG GGCAGGCAGC CGGGCGCCCC CGGCCCCCTC 
GACGGCGGCT GCGCTGCGGC GGCAAAGCTC AAGAGCAGCA CGGCGCCGGG GCCCGGGGGC 
CAGCCCGACT TTGGCCTGCT GCCGGGGCCC GGCGAGTCAG CGGGCGGCGC CGGGAGCGGC 
CTGGCCACCC AAGACCTGGC AGGACAGGGG TACCTGGCCC ATCCGCTGCT CCACGACGGC 
GTGCCCCACC AAGGGGCGGG CCGCGCAGGC CAGCAGATGC TAGGGCAGGT TAGCGCTACC 
TCACACGTCA ACGTCTATCT GGGGCCCGAG GGCGGCCTGC CAGGGCCGCC GTGCGTCAGC 
GGCCAGCCCG CCGGCTTGGC GGCCACCCGG GGCTACCAGC CGTGTGCGGC CTACGGGGGC 
GGCAGGCGCC AGGCTGTGCC GAGGGGCAGC CTCGCTCTGC AGCAGGCACA GCTCAGTGAC 
CCAAGTCCGA CCTGCAGGGT GAACGGCATC AAGGTGGAGA TGCAGGGGCA GCCCCATCAG 
CTCTGTTCGA ACACGCAGAG TTACTCTGGT CAGTTCTATG ACCAAACCGT TGGCTTCAGT 
CAGCAAGACA TGAAAACTGG TTCATTCTCC ATTTCAGAAG CCAGCTGCCT GCTACAGGGG 
GCCAGCGCCG AGAACTCTGA ATTACTTTCC CCAGGTGCTA ACCAGGTGAC GAGCACGGTT 
GACAGCCTCG ACAGTCATGA CCTGGAAGGG GTGCAGATCG ATTTTGACGC CATCATAGAC 
GACGGGGACC ACGCCAGCCT GATGTCAGGA GCCCTGAGTC CCAGTATCAT CCAGAACCTC 
TCCCATAGCT CCTCCCGCCT CACGACACCC CGCGCGTCCC TCCCGCTGCC CACGCTGTCC 
GTGAGCACCA CCAACATGGC CATCGGGGAC ATGAGCTCCC TGCTGACCTC CCTGGCGGAA 
GAAAGCAAAT TCCTTGCAGT GATGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_540363.2
CDS	132..4901
Translation	MEAQNHSSTTTEKKKVDTSIVKCSSRTDVSEKAVASSTTSNEDESPGQTYHRERRNAVTMQPQSVQGLGKISEEPSTSSEERASLIKKEIHGSLPHLAEPSVPYRGAVFAMDPRNGYMEPHYHPSHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASEPPFSPPHPYINPYMDYIRSLHSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVTSGPSESSQNKPTSESAVSSTGDLMHNKRSKIKPDEDLPSPGPRGQQEQPEGTTLVKEEGDRDESKQEPEVIYETNCHWEGCTREFDTQEQLVHHINNDHIHGEKKEFVCRWMDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRQTQGALGEQKDLSNTTSKREECLQVKAVKAEKPMTSQPSPGGESSCSSQQSPISNYSNSGLELPLTDGGGVGDLGTIDETPIMDSTISTATTALALQARRNPAGAKWMEHVKLERLKQVNGMLPRLNPVLPPKAPAVSPLIGNGTQPSTSCSLGGPITLLPNRSDLSGVDITMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDAREPAGALPPVPPPRRCSDGGAHGYGRRPLLPLDALGNGVRRASDPVRTASDGLSLPRVQRFGSLHSFNPPGPPPTLEKRHLVLQNYTRPDGGQPRHFPPSPCPPSITENVTLEALTMDADVSLTDEDFLPDDVVQYLNSQNPAGFEQPFQSAVSEDSKAPHGPGDFDGPGLPDGHAGHPGHPGHPGPAGQPYRPLEQPCPEASKADLPIQWNEVSSGSADLCSSKLKCGQRPAAQQARAFGFYGNVGAPPQPALRSGPGPAGAYAPLAEPGPAYGGPEPLAGHGAGAGSPGDAFHEQPCKAPQFGSCLGRQPGAPGPLDGGCAAAAKLKSSTAPGPGGQPDFGLLPGPGESAGGAGSGLATQDLAGQGYLAHPLLHDGVPHQGAGRAGQQMLGQVSATSHVNVYLGPEGGLPGPPCVSGQPAGLAATRGYQPCAAYGGGRRQAVPRGSLALQQAQLSDPSPTCRVNGIKVEMQGQPHQLCSNTQSYSGQFYDQTVGFSQQDMKTGSFSISEASCLLQGASAENSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHASLMSGALSPSIIQNLSHSSSRLTTPRASLPLPTLSVSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_540363.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_540363.5

ORF Insert Sequence:

ATGGAGGCCC AGAACCACAG CTCCACGACC ACTGAGAAGA AGAAAGTGGA CACTTCCATA 
GTGAAATGCT CCTCGAGAAC AGATGTCAGC GAGAAAGCGG TGGCCTCCAG CACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG 
CAGCCACAGA GCGTCCAGGG GCTCGGCAAG ATCAGCGAGG AGCCTTCGAC GTCTAGCGAG 
GAGAGGGCCT CGCTGATCAA GAAGGAGATC CATGGGTCCC TGCCGCACCT GGCCGAGCCC 
TCGGTGCCGT ACCGTGGGGC CGTGTTTGCC ATGGACCCCC GGAACGGTTA CATGGAGCCC 
CACTACCACC CTTCTCATCT CTTTCCTGCA TTCCACCCTC CTGTACCAAT CGATGCAAGA 
CATCATGAGG GGCGTTACCA TTATGACCCG TCTCCGATTC CTCCATTGCA TGTGCCTTCT 
GCTTTATCCA GTAGCCCAAC GTATTCGGAC CTGCCCTTCA TCAGGATCTC CCCACACCGG 
AACCCCGCTG CAGCCTCTGA ACCCCCCTTC AGCCCTCCAC ACCCCTACAT CAACCCCTAC 
ATGGACTACA TCCGGTCCCT ACACAGCCCG TCCCTCTCCA TGATCTCGGC AGCTCGGGGG 
CTCAGCCCTA CGGATGCCCC CCATGCTGGG GTCAGCCCAG CGGAATACTA TCATCAGATG 
GCTCTGCTGG CTGGCCAGCG CAGCCCCTAC GCAGACATCA TTCCCTCCGC TGCCACTGCC 
GGCGCTGGGG CCATCCACAT GGAATATCTT CATGCCATGG ACAGCACCAG ATTCCCCAGC 
CCCAGGCTGT CGGCCCGGCC GAGCCGAAAA CGTACACTGT CCATATCACC GCTGTCCGAT 
CACAGCTTTG ACCTTCAGAC CATGATAAGG ACATCTCCCA ACTCCTTGGT CACGATTCTC 
AATAATTCCC GCAGCAGCTC TTCAGCGAGC GGCTCCTACG GCCACTTGTC TGCGAGTGCA 
ATCAGCCCTG CCCTGAGCTT CACCTACCCT TCTGCACCCG TGTCTCTCCA CATGCATCAG 
CAAATCTTAA GCCGACAACA GAGCCTAGGC TCGGCCTTTG GACACAGCCC TCCGCTCATC 
CACCCTGCTC CAACTTTTCC AACGCAGAGG CCTATTCCTG GGATCCCTAC GGTGCTCAAC 
CCCGTCCAGG TCACTTCTGG CCCTTCTGAG TCCTCACAGA ACAAGCCGAC GAGTGAGTCC 
GCGGTGAGTA GCACTGGTGA CCTGATGCAC AACAAGCGGT CCAAGATCAA ACCTGACGAA 
GACCTACCCA GCCCGGGGCC ACGGGGCCAG CAGGAACAGC CAGAAGGAAC AACCCTGGTA 
AAGGAAGAGG GTGACAGAGA CGAAAGCAAA CAAGAGCCCG AAGTCATCTA TGAGACCAAC 
TGCCACTGGG AAGGTTGCAC GCGGGAGTTT GACACCCAGG AGCAGCTGGT GCACCATATA 
AATAATGACC ATATTCACGG CGAGAAGAAG GAGTTCGTGT GCCGGTGGAT GGATTGCTCG 
AGGGAGCAGA AGCCCTTCAA AGCCCAGTAC ATGCTGGTGG TGCACATGAG GAGACACACC 
GGGGAGAAAC CTCACAAATG CACTTTTGAA GGTTGTACAA AGGCCTACTC GAGACTAGAA 
AACTTGAAAA CGCACTTGAG ATCTCACACT GGAGAGAAAC CGTACGTCTG TGAGCACGAA 
GGTTGCAACA AGGCTTTCTC AAATGCCTCT GATCGCGCCA AGCACCAAAA CAGGACGCAT 
TCCAATGAGA AACCCTATGT GTGCAAAATC CCAGGCTGCA CCAAGCGCTA CACAGACCCA 
AGTTCCCTCC GGAAACATGT GAAGACCGTG CATGGCCCAG AGGCTCATGT CACCAAGAAG 
CAGCGTGGGG ACATACATCC TCGGCCCCCC CCACCCCGAG ATTCGGGCAG CCATTCACAG 
TCCAGGTCGC CTGGCCGGCA GACTCAGGGA GCCCTTGGGG AGCAGAAGGA CCTCAGCAAC 
ACTACCTCAA AGCGGGAAGA ATGCCTCCAA GTGAAAGCTG TCAAGGCCGA GAAGCCCATG 
ACATCTCAGC CAAGCCCTGG TGGTGAGTCT TCATGCAGCA GCCAACAGTC CCCCATCAGC 
AACTATTCCA ACAGTGGGCT CGAGCTTCCT CTGACCGACG GAGGTGGCGT GGGAGACCTC 
GGGACCATCG ACGAAACCCC AATCATGGAC TCGACCATTT CCACCGCAAC CACCGCCCTC 
GCCTTGCAAG CCAGGAGGAA CCCGGCAGGG GCCAAATGGA TGGAGCATGT GAAACTAGAA 
AGGCTCAAAC AAGTGAATGG AATGCTTCCA CGACTGAACC CCGTTCTTCC CCCCAAAGCC 
CCTGCAGTCT CTCCTCTCAT AGGAAACGGC ACTCAGCCCA GCACCAGCTG CAGTTTGGGC 
GGGCCCATAA CCCTCCTCCC GAACAGGAGC GACCTCTCCG GGGTGGACAT CACCATGCTG 
AACATGCTCA ACCGGAGGGA CAGCAGCGCC AGCACCATCA GCTCCGCCTA CCTGAGCAGC 
CGCCGCTCCT CGGGCATCTC CCCCTGCTTT TCCAGCCGCC GGTCCAGCGA GGCGTCCCAG 
GCCGAGGGCC GGCCCCAGAA CGTGAGCGTG GCCGACTCCT ACGACCCCAT CTCCACCGAC 
GCGTCGCGCA GGTCCAGCGA GGCGAGCCAG GGCGACGGGC TGCCCAGCCT GCTCAGCCTC 
ACGCCGGCGC AGCAGTACCG CCTGAAGGCC AAGTACGCGG CGGCCACGGG CGGGCCGCCC 
CCCACCCCGC TGCCCAACAT GGAGAGGATG AGCCTCAAGA CCAGGATGGC CCTGCTCGGG 
GACGCCCGGG AGCCCGCGGG GGCCCTGCCG CCCGTGCCCC CTCCGCGGCG GTGCAGCGAC 
GGCGGCGCCC ACGGCTACGG CCGCCGCCCC CTGCTGCCCC TGGACGCGCT GGGCAACGGC 
GTGCGCAGGG CCAGCGACCC GGTGCGGACG GCGTCCGACG GCCTCTCCCT GCCCCGCGTG 
CAGCGCTTCG GCAGCCTTCA CAGCTTCAAC CCGCCCGGGC CGCCTCCCAC CCTGGAGAAG 
CGGCACCTGG TGCTCCAGAA TTACACGCGG CCCGACGGCG GCCAGCCCCG CCACTTCCCG 
CCGTCCCCCT GTCCGCCGAG CATCACCGAG AACGTCACCC TGGAGGCCCT GACCATGGAC 
GCCGACGTGA GCCTCACCGA TGAGGACTTC CTGCCCGACG ACGTGGTGCA GTATCTGAAT 
TCCCAGAACC CGGCCGGGTT CGAGCAGCCC TTCCAGAGCG CCGTCTCCGA GGACAGCAAA 
GCGCCCCACG GGCCCGGCGA CTTCGACGGC CCGGGGCTGC CGGACGGTCA CGCGGGCCAC 
CCAGGCCACC CGGGCCACCC GGGCCCCGCC GGCCAGCCGT ACCGCCCGCT GGAGCAGCCG 
TGCCCCGAAG CCAGCAAAGC CGACCTGCCC ATTCAGTGGA ACGAAGTGAG CTCGGGCAGC 
GCCGACCTGT GCTCCTCCAA GCTCAAGTGT GGCCAGCGGC CGGCGGCGCA GCAGGCCCGG 
GCCTTCGGCT TCTACGGCAA CGTGGGCGCC CCCCCGCAGC CCGCCTTGCG GAGCGGGCCC 
GGCCCCGCCG GCGCCTATGC CCCCCTGGCC GAGCCCGGCC CGGCCTACGG CGGCCCCGAG 
CCCTTGGCGG GCCACGGCGC TGGAGCGGGC AGCCCCGGAG ACGCGTTCCA CGAACAGCCC 
TGCAAGGCCC CGCAGTTCGG GAGCTGCCTG GGCAGGCAGC CGGGCGCCCC CGGCCCCCTC 
GACGGCGGCT GCGCTGCGGC GGCAAAGCTC AAGAGCAGCA CGGCGCCGGG GCCCGGGGGC 
CAGCCCGACT TTGGCCTGCT GCCGGGGCCC GGCGAGTCAG CGGGCGGCGC CGGGAGCGGC 
CTGGCCACCC AAGACCTGGC AGGACAGGGG TACCTGGCCC ATCCGCTGCT CCACGACGGC 
GTGCCCCACC AAGGGGCGGG CCGCGCAGGC CAGCAGATGC TAGGGCAGGT TAGCGCTACC 
TCACACGTCA ACGTCTATCT GGGGCCCGAG GGCGGCCTGC CAGGGCCGCC GTGCGTCAGC 
GGCCAGCCCG CCGGCTTGGC GGCCACCCGG GGCTACCAGC CGTGTGCGGC CTACGGGGGC 
GGCAGGCGCC AGGCTGTGCC GAGGGGCAGC CTCGCTCTGC AGCAGGCACA GCTCAGTGAC 
CCAAGTCCGA CCTGCAGGGT GAACGGCATC AAGGTGGAGA TGCAGGGGCA GCCCCATCAG 
CTCTGTTCGA ACACGCAGAG TTACTCTGGT CAGTTCTATG ACCAAACCGT TGGCTTCAGT 
CAGCAAGACA TGAAAACTGG TTCATTCTCC ATTTCAGAAG CCAGCTGCCT GCTACAGGGG 
GCCAGCGCCG AGAACTCTGA ATTACTTTCC CCAGGTGCTA ACCAGGTGAC GAGCACGGTT 
GACAGCCTCG ACAGTCATGA CCTGGAAGGG GTGCAGATCG ATTTTGACGC CATCATAGAC 
GACGGGGACC ACGCCAGCCT GATGTCAGGA GCCCTGAGTC CCAGTATCAT CCAGAACCTC 
TCCCATAGCT CCTCCCGCCT CACGACACCC CGCGCGTCCC TCCCGCTGCC CACGCTGTCC 
GTGAGCACCA CCAACATGGC CATCGGGGAC ATGAGCTCCC TGCTGACCTC CCTGGCGGAA 
GAAAGCAAAT TCCTTGCAGT GATGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_540363.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_540363.2 Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe19652
Clone ID Related Accession (Same CDS sequence)	XM_540363.5 , XM_540363.4
Accession Version	XM_540363.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	transcriptional activator GLI3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726091.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_540363.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC AGAACCACAG CTCCACGACC ACTGAGAAGA AGAAAGTGGA CACTTCCATA 
GTGAAATGCT CCTCGAGAAC AGATGTCAGC GAGAAAGCGG TGGCCTCCAG CACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG 
CAGCCACAGA GCGTCCAGGG GCTCGGCAAG ATCAGCGAGG AGCCTTCGAC GTCTAGCGAG 
GAGAGGGCCT CGCTGATCAA GAAGGAGATC CATGGGTCCC TGCCGCACCT GGCCGAGCCC 
TCGGTGCCGT ACCGTGGGGC CGTGTTTGCC ATGGACCCCC GGAACGGTTA CATGGAGCCC 
CACTACCACC CTTCTCATCT CTTTCCTGCA TTCCACCCTC CTGTACCAAT CGATGCAAGA 
CATCATGAGG GGCGTTACCA TTATGACCCG TCTCCGATTC CTCCATTGCA TGTGCCTTCT 
GCTTTATCCA GTAGCCCAAC GTATTCGGAC CTGCCCTTCA TCAGGATCTC CCCACACCGG 
AACCCCGCTG CAGCCTCTGA ACCCCCCTTC AGCCCTCCAC ACCCCTACAT CAACCCCTAC 
ATGGACTACA TCCGGTCCCT ACACAGCCCG TCCCTCTCCA TGATCTCGGC AGCTCGGGGG 
CTCAGCCCTA CGGATGCCCC CCATGCTGGG GTCAGCCCAG CGGAATACTA TCATCAGATG 
GCTCTGCTGG CTGGCCAGCG CAGCCCCTAC GCAGACATCA TTCCCTCCGC TGCCACTGCC 
GGCGCTGGGG CCATCCACAT GGAATATCTT CATGCCATGG ACAGCACCAG ATTCCCCAGC 
CCCAGGCTGT CGGCCCGGCC GAGCCGAAAA CGTACACTGT CCATATCACC GCTGTCCGAT 
CACAGCTTTG ACCTTCAGAC CATGATAAGG ACATCTCCCA ACTCCTTGGT CACGATTCTC 
AATAATTCCC GCAGCAGCTC TTCAGCGAGC GGCTCCTACG GCCACTTGTC TGCGAGTGCA 
ATCAGCCCTG CCCTGAGCTT CACCTACCCT TCTGCACCCG TGTCTCTCCA CATGCATCAG 
CAAATCTTAA GCCGACAACA GAGCCTAGGC TCGGCCTTTG GACACAGCCC TCCGCTCATC 
CACCCTGCTC CAACTTTTCC AACGCAGAGG CCTATTCCTG GGATCCCTAC GGTGCTCAAC 
CCCGTCCAGG TCACTTCTGG CCCTTCTGAG TCCTCACAGA ACAAGCCGAC GAGTGAGTCC 
GCGGTGAGTA GCACTGGTGA CCTGATGCAC AACAAGCGGT CCAAGATCAA ACCTGACGAA 
GACCTACCCA GCCCGGGGCC ACGGGGCCAG CAGGAACAGC CAGAAGGAAC AACCCTGGTA 
AAGGAAGAGG GTGACAGAGA CGAAAGCAAA CAAGAGCCCG AAGTCATCTA TGAGACCAAC 
TGCCACTGGG AAGGTTGCAC GCGGGAGTTT GACACCCAGG AGCAGCTGGT GCACCATATA 
AATAATGACC ATATTCACGG CGAGAAGAAG GAGTTCGTGT GCCGGTGGAT GGATTGCTCG 
AGGGAGCAGA AGCCCTTCAA AGCCCAGTAC ATGCTGGTGG TGCACATGAG GAGACACACC 
GGGGAGAAAC CTCACAAATG CACTTTTGAA GGTTGTACAA AGGCCTACTC GAGACTAGAA 
AACTTGAAAA CGCACTTGAG ATCTCACACT GGAGAGAAAC CGTACGTCTG TGAGCACGAA 
GGTTGCAACA AGGCTTTCTC AAATGCCTCT GATCGCGCCA AGCACCAAAA CAGGACGCAT 
TCCAATGAGA AACCCTATGT GTGCAAAATC CCAGGCTGCA CCAAGCGCTA CACAGACCCA 
AGTTCCCTCC GGAAACATGT GAAGACCGTG CATGGCCCAG AGGCTCATGT CACCAAGAAG 
CAGCGTGGGG ACATACATCC TCGGCCCCCC CCACCCCGAG ATTCGGGCAG CCATTCACAG 
TCCAGGTCGC CTGGCCGGCA GACTCAGGGA GCCCTTGGGG AGCAGAAGGA CCTCAGCAAC 
ACTACCTCAA AGCGGGAAGA ATGCCTCCAA GTGAAAGCTG TCAAGGCCGA GAAGCCCATG 
ACATCTCAGC CAAGCCCTGG TGGTGAGTCT TCATGCAGCA GCCAACAGTC CCCCATCAGC 
AACTATTCCA ACAGTGGGCT CGAGCTTCCT CTGACCGACG GAGGTGGCGT GGGAGACCTC 
GGGACCATCG ACGAAACCCC AATCATGGAC TCGACCATTT CCACCGCAAC CACCGCCCTC 
GCCTTGCAAG CCAGGAGGAA CCCGGCAGGG GCCAAATGGA TGGAGCATGT GAAACTAGAA 
AGGCTCAAAC AAGTGAATGG AATGCTTCCA CGACTGAACC CCGTTCTTCC CCCCAAAGCC 
CCTGCAGTCT CTCCTCTCAT AGGAAACGGC ACTCAGCCCA GCACCAGCTG CAGTTTGGGC 
GGGCCCATAA CCCTCCTCCC GAACAGGAGC GACCTCTCCG GGGTGGACAT CACCATGCTG 
AACATGCTCA ACCGGAGGGA CAGCAGCGCC AGCACCATCA GCTCCGCCTA CCTGAGCAGC 
CGCCGCTCCT CGGGCATCTC CCCCTGCTTT TCCAGCCGCC GGTCCAGCGA GGCGTCCCAG 
GCCGAGGGCC GGCCCCAGAA CGTGAGCGTG GCCGACTCCT ACGACCCCAT CTCCACCGAC 
GCGTCGCGCA GGTCCAGCGA GGCGAGCCAG GGCGACGGGC TGCCCAGCCT GCTCAGCCTC 
ACGCCGGCGC AGCAGTACCG CCTGAAGGCC AAGTACGCGG CGGCCACGGG CGGGCCGCCC 
CCCACCCCGC TGCCCAACAT GGAGAGGATG AGCCTCAAGA CCAGGATGGC CCTGCTCGGG 
GACGCCCGGG AGCCCGCGGG GGCCCTGCCG CCCGTGCCCC CTCCGCGGCG GTGCAGCGAC 
GGCGGCGCCC ACGGCTACGG CCGCCGCCCC CTGCTGCCCC TGGACGCGCT GGGCAACGGC 
GTGCGCAGGG CCAGCGACCC GGTGCGGACG GCGTCCGACG GCCTCTCCCT GCCCCGCGTG 
CAGCGCTTCG GCAGCCTTCA CAGCTTCAAC CCGCCCGGGC CGCCTCCCAC CCTGGAGAAG 
CGGCACCTGG TGCTCCAGAA TTACACGCGG CCCGACGGCG GCCAGCCCCG CCACTTCCCG 
CCGTCCCCCT GTCCGCCGAG CATCACCGAG AACGTCACCC TGGAGGCCCT GACCATGGAC 
GCCGACGTGA GCCTCACCGA TGAGGACTTC CTGCCCGACG ACGTGGTGCA GTATCTGAAT 
TCCCAGAACC CGGCCGGGTT CGAGCAGCCC TTCCAGAGCG CCGTCTCCGA GGACAGCAAA 
GCGCCCCACG GGCCCGGCGA CTTCGACGGC CCGGGGCTGC CGGACGGTCA CGCGGGCCAC 
CCAGGCCACC CGGGCCACCC GGGCCCCGCC GGCCAGCCGT ACCGCCCGCT GGAGCAGCCG 
TGCCCCGAAG CCAGCAAAGC CGACCTGCCC ATTCAGTGGA ACGAAGTGAG CTCGGGCAGC 
GCCGACCTGT GCTCCTCCAA GCTCAAGTGT GGCCAGCGGC CGGCGGCGCA GCAGGCCCGG 
GCCTTCGGCT TCTACGGCAA CGTGGGCGCC CCCCCGCAGC CCGCCTTGCG GAGCGGGCCC 
GGCCCCGCCG GCGCCTATGC CCCCCTGGCC GAGCCCGGCC CGGCCTACGG CGGCCCCGAG 
CCCTTGGCGG GCCACGGCGC TGGAGCGGGC AGCCCCGGAG ACGCGTTCCA CGAACAGCCC 
TGCAAGGCCC CGCAGTTCGG GAGCTGCCTG GGCAGGCAGC CGGGCGCCCC CGGCCCCCTC 
GACGGCGGCT GCGCTGCGGC GGCAAAGCTC AAGAGCAGCA CGGCGCCGGG GCCCGGGGGC 
CAGCCCGACT TTGGCCTGCT GCCGGGGCCC GGCGAGTCAG CGGGCGGCGC CGGGAGCGGC 
CTGGCCACCC AAGACCTGGC AGGACAGGGG TACCTGGCCC ATCCGCTGCT CCACGACGGC 
GTGCCCCACC AAGGGGCGGG CCGCGCAGGC CAGCAGATGC TAGGGCAGGT TAGCGCTACC 
TCACACGTCA ACGTCTATCT GGGGCCCGAG GGCGGCCTGC CAGGGCCGCC GTGCGTCAGC 
GGCCAGCCCG CCGGCTTGGC GGCCACCCGG GGCTACCAGC CGTGTGCGGC CTACGGGGGC 
GGCAGGCGCC AGGCTGTGCC GAGGGGCAGC CTCGCTCTGC AGCAGGCACA GCTCAGTGAC 
CCAAGTCCGA CCTGCAGGGT GAACGGCATC AAGGTGGAGA TGCAGGGGCA GCCCCATCAG 
CTCTGTTCGA ACACGCAGAG TTACTCTGGT CAGTTCTATG ACCAAACCGT TGGCTTCAGT 
CAGCAAGACA TGAAAACTGG TTCATTCTCC ATTTCAGAAG CCAGCTGCCT GCTACAGGGG 
GCCAGCGCCG AGAACTCTGA ATTACTTTCC CCAGGTGCTA ACCAGGTGAC GAGCACGGTT 
GACAGCCTCG ACAGTCATGA CCTGGAAGGG GTGCAGATCG ATTTTGACGC CATCATAGAC 
GACGGGGACC ACGCCAGCCT GATGTCAGGA GCCCTGAGTC CCAGTATCAT CCAGAACCTC 
TCCCATAGCT CCTCCCGCCT CACGACACCC CGCGCGTCCC TCCCGCTGCC CACGCTGTCC 
GTGAGCACCA CCAACATGGC CATCGGGGAC ATGAGCTCCC TGCTGACCTC CCTGGCGGAA 
GAAAGCAAAT TCCTTGCAGT GATGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_540363.2
CDS	119..4888
Translation	MEAQNHSSTTTEKKKVDTSIVKCSSRTDVSEKAVASSTTSNEDESPGQTYHRERRNAVTMQPQSVQGLGKISEEPSTSSEERASLIKKEIHGSLPHLAEPSVPYRGAVFAMDPRNGYMEPHYHPSHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASEPPFSPPHPYINPYMDYIRSLHSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVTSGPSESSQNKPTSESAVSSTGDLMHNKRSKIKPDEDLPSPGPRGQQEQPEGTTLVKEEGDRDESKQEPEVIYETNCHWEGCTREFDTQEQLVHHINNDHIHGEKKEFVCRWMDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRQTQGALGEQKDLSNTTSKREECLQVKAVKAEKPMTSQPSPGGESSCSSQQSPISNYSNSGLELPLTDGGGVGDLGTIDETPIMDSTISTATTALALQARRNPAGAKWMEHVKLERLKQVNGMLPRLNPVLPPKAPAVSPLIGNGTQPSTSCSLGGPITLLPNRSDLSGVDITMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDAREPAGALPPVPPPRRCSDGGAHGYGRRPLLPLDALGNGVRRASDPVRTASDGLSLPRVQRFGSLHSFNPPGPPPTLEKRHLVLQNYTRPDGGQPRHFPPSPCPPSITENVTLEALTMDADVSLTDEDFLPDDVVQYLNSQNPAGFEQPFQSAVSEDSKAPHGPGDFDGPGLPDGHAGHPGHPGHPGPAGQPYRPLEQPCPEASKADLPIQWNEVSSGSADLCSSKLKCGQRPAAQQARAFGFYGNVGAPPQPALRSGPGPAGAYAPLAEPGPAYGGPEPLAGHGAGAGSPGDAFHEQPCKAPQFGSCLGRQPGAPGPLDGGCAAAAKLKSSTAPGPGGQPDFGLLPGPGESAGGAGSGLATQDLAGQGYLAHPLLHDGVPHQGAGRAGQQMLGQVSATSHVNVYLGPEGGLPGPPCVSGQPAGLAATRGYQPCAAYGGGRRQAVPRGSLALQQAQLSDPSPTCRVNGIKVEMQGQPHQLCSNTQSYSGQFYDQTVGFSQQDMKTGSFSISEASCLLQGASAENSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHASLMSGALSPSIIQNLSHSSSRLTTPRASLPLPTLSVSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_540363.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_540363.4

ORF Insert Sequence:

ATGGAGGCCC AGAACCACAG CTCCACGACC ACTGAGAAGA AGAAAGTGGA CACTTCCATA 
GTGAAATGCT CCTCGAGAAC AGATGTCAGC GAGAAAGCGG TGGCCTCCAG CACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG 
CAGCCACAGA GCGTCCAGGG GCTCGGCAAG ATCAGCGAGG AGCCTTCGAC GTCTAGCGAG 
GAGAGGGCCT CGCTGATCAA GAAGGAGATC CATGGGTCCC TGCCGCACCT GGCCGAGCCC 
TCGGTGCCGT ACCGTGGGGC CGTGTTTGCC ATGGACCCCC GGAACGGTTA CATGGAGCCC 
CACTACCACC CTTCTCATCT CTTTCCTGCA TTCCACCCTC CTGTACCAAT CGATGCAAGA 
CATCATGAGG GGCGTTACCA TTATGACCCG TCTCCGATTC CTCCATTGCA TGTGCCTTCT 
GCTTTATCCA GTAGCCCAAC GTATTCGGAC CTGCCCTTCA TCAGGATCTC CCCACACCGG 
AACCCCGCTG CAGCCTCTGA ACCCCCCTTC AGCCCTCCAC ACCCCTACAT CAACCCCTAC 
ATGGACTACA TCCGGTCCCT ACACAGCCCG TCCCTCTCCA TGATCTCGGC AGCTCGGGGG 
CTCAGCCCTA CGGATGCCCC CCATGCTGGG GTCAGCCCAG CGGAATACTA TCATCAGATG 
GCTCTGCTGG CTGGCCAGCG CAGCCCCTAC GCAGACATCA TTCCCTCCGC TGCCACTGCC 
GGCGCTGGGG CCATCCACAT GGAATATCTT CATGCCATGG ACAGCACCAG ATTCCCCAGC 
CCCAGGCTGT CGGCCCGGCC GAGCCGAAAA CGTACACTGT CCATATCACC GCTGTCCGAT 
CACAGCTTTG ACCTTCAGAC CATGATAAGG ACATCTCCCA ACTCCTTGGT CACGATTCTC 
AATAATTCCC GCAGCAGCTC TTCAGCGAGC GGCTCCTACG GCCACTTGTC TGCGAGTGCA 
ATCAGCCCTG CCCTGAGCTT CACCTACCCT TCTGCACCCG TGTCTCTCCA CATGCATCAG 
CAAATCTTAA GCCGACAACA GAGCCTAGGC TCGGCCTTTG GACACAGCCC TCCGCTCATC 
CACCCTGCTC CAACTTTTCC AACGCAGAGG CCTATTCCTG GGATCCCTAC GGTGCTCAAC 
CCCGTCCAGG TCACTTCTGG CCCTTCTGAG TCCTCACAGA ACAAGCCGAC GAGTGAGTCC 
GCGGTGAGTA GCACTGGTGA CCTGATGCAC AACAAGCGGT CCAAGATCAA ACCTGACGAA 
GACCTACCCA GCCCGGGGCC ACGGGGCCAG CAGGAACAGC CAGAAGGAAC AACCCTGGTA 
AAGGAAGAGG GTGACAGAGA CGAAAGCAAA CAAGAGCCCG AAGTCATCTA TGAGACCAAC 
TGCCACTGGG AAGGTTGCAC GCGGGAGTTT GACACCCAGG AGCAGCTGGT GCACCATATA 
AATAATGACC ATATTCACGG CGAGAAGAAG GAGTTCGTGT GCCGGTGGAT GGATTGCTCG 
AGGGAGCAGA AGCCCTTCAA AGCCCAGTAC ATGCTGGTGG TGCACATGAG GAGACACACC 
GGGGAGAAAC CTCACAAATG CACTTTTGAA GGTTGTACAA AGGCCTACTC GAGACTAGAA 
AACTTGAAAA CGCACTTGAG ATCTCACACT GGAGAGAAAC CGTACGTCTG TGAGCACGAA 
GGTTGCAACA AGGCTTTCTC AAATGCCTCT GATCGCGCCA AGCACCAAAA CAGGACGCAT 
TCCAATGAGA AACCCTATGT GTGCAAAATC CCAGGCTGCA CCAAGCGCTA CACAGACCCA 
AGTTCCCTCC GGAAACATGT GAAGACCGTG CATGGCCCAG AGGCTCATGT CACCAAGAAG 
CAGCGTGGGG ACATACATCC TCGGCCCCCC CCACCCCGAG ATTCGGGCAG CCATTCACAG 
TCCAGGTCGC CTGGCCGGCA GACTCAGGGA GCCCTTGGGG AGCAGAAGGA CCTCAGCAAC 
ACTACCTCAA AGCGGGAAGA ATGCCTCCAA GTGAAAGCTG TCAAGGCCGA GAAGCCCATG 
ACATCTCAGC CAAGCCCTGG TGGTGAGTCT TCATGCAGCA GCCAACAGTC CCCCATCAGC 
AACTATTCCA ACAGTGGGCT CGAGCTTCCT CTGACCGACG GAGGTGGCGT GGGAGACCTC 
GGGACCATCG ACGAAACCCC AATCATGGAC TCGACCATTT CCACCGCAAC CACCGCCCTC 
GCCTTGCAAG CCAGGAGGAA CCCGGCAGGG GCCAAATGGA TGGAGCATGT GAAACTAGAA 
AGGCTCAAAC AAGTGAATGG AATGCTTCCA CGACTGAACC CCGTTCTTCC CCCCAAAGCC 
CCTGCAGTCT CTCCTCTCAT AGGAAACGGC ACTCAGCCCA GCACCAGCTG CAGTTTGGGC 
GGGCCCATAA CCCTCCTCCC GAACAGGAGC GACCTCTCCG GGGTGGACAT CACCATGCTG 
AACATGCTCA ACCGGAGGGA CAGCAGCGCC AGCACCATCA GCTCCGCCTA CCTGAGCAGC 
CGCCGCTCCT CGGGCATCTC CCCCTGCTTT TCCAGCCGCC GGTCCAGCGA GGCGTCCCAG 
GCCGAGGGCC GGCCCCAGAA CGTGAGCGTG GCCGACTCCT ACGACCCCAT CTCCACCGAC 
GCGTCGCGCA GGTCCAGCGA GGCGAGCCAG GGCGACGGGC TGCCCAGCCT GCTCAGCCTC 
ACGCCGGCGC AGCAGTACCG CCTGAAGGCC AAGTACGCGG CGGCCACGGG CGGGCCGCCC 
CCCACCCCGC TGCCCAACAT GGAGAGGATG AGCCTCAAGA CCAGGATGGC CCTGCTCGGG 
GACGCCCGGG AGCCCGCGGG GGCCCTGCCG CCCGTGCCCC CTCCGCGGCG GTGCAGCGAC 
GGCGGCGCCC ACGGCTACGG CCGCCGCCCC CTGCTGCCCC TGGACGCGCT GGGCAACGGC 
GTGCGCAGGG CCAGCGACCC GGTGCGGACG GCGTCCGACG GCCTCTCCCT GCCCCGCGTG 
CAGCGCTTCG GCAGCCTTCA CAGCTTCAAC CCGCCCGGGC CGCCTCCCAC CCTGGAGAAG 
CGGCACCTGG TGCTCCAGAA TTACACGCGG CCCGACGGCG GCCAGCCCCG CCACTTCCCG 
CCGTCCCCCT GTCCGCCGAG CATCACCGAG AACGTCACCC TGGAGGCCCT GACCATGGAC 
GCCGACGTGA GCCTCACCGA TGAGGACTTC CTGCCCGACG ACGTGGTGCA GTATCTGAAT 
TCCCAGAACC CGGCCGGGTT CGAGCAGCCC TTCCAGAGCG CCGTCTCCGA GGACAGCAAA 
GCGCCCCACG GGCCCGGCGA CTTCGACGGC CCGGGGCTGC CGGACGGTCA CGCGGGCCAC 
CCAGGCCACC CGGGCCACCC GGGCCCCGCC GGCCAGCCGT ACCGCCCGCT GGAGCAGCCG 
TGCCCCGAAG CCAGCAAAGC CGACCTGCCC ATTCAGTGGA ACGAAGTGAG CTCGGGCAGC 
GCCGACCTGT GCTCCTCCAA GCTCAAGTGT GGCCAGCGGC CGGCGGCGCA GCAGGCCCGG 
GCCTTCGGCT TCTACGGCAA CGTGGGCGCC CCCCCGCAGC CCGCCTTGCG GAGCGGGCCC 
GGCCCCGCCG GCGCCTATGC CCCCCTGGCC GAGCCCGGCC CGGCCTACGG CGGCCCCGAG 
CCCTTGGCGG GCCACGGCGC TGGAGCGGGC AGCCCCGGAG ACGCGTTCCA CGAACAGCCC 
TGCAAGGCCC CGCAGTTCGG GAGCTGCCTG GGCAGGCAGC CGGGCGCCCC CGGCCCCCTC 
GACGGCGGCT GCGCTGCGGC GGCAAAGCTC AAGAGCAGCA CGGCGCCGGG GCCCGGGGGC 
CAGCCCGACT TTGGCCTGCT GCCGGGGCCC GGCGAGTCAG CGGGCGGCGC CGGGAGCGGC 
CTGGCCACCC AAGACCTGGC AGGACAGGGG TACCTGGCCC ATCCGCTGCT CCACGACGGC 
GTGCCCCACC AAGGGGCGGG CCGCGCAGGC CAGCAGATGC TAGGGCAGGT TAGCGCTACC 
TCACACGTCA ACGTCTATCT GGGGCCCGAG GGCGGCCTGC CAGGGCCGCC GTGCGTCAGC 
GGCCAGCCCG CCGGCTTGGC GGCCACCCGG GGCTACCAGC CGTGTGCGGC CTACGGGGGC 
GGCAGGCGCC AGGCTGTGCC GAGGGGCAGC CTCGCTCTGC AGCAGGCACA GCTCAGTGAC 
CCAAGTCCGA CCTGCAGGGT GAACGGCATC AAGGTGGAGA TGCAGGGGCA GCCCCATCAG 
CTCTGTTCGA ACACGCAGAG TTACTCTGGT CAGTTCTATG ACCAAACCGT TGGCTTCAGT 
CAGCAAGACA TGAAAACTGG TTCATTCTCC ATTTCAGAAG CCAGCTGCCT GCTACAGGGG 
GCCAGCGCCG AGAACTCTGA ATTACTTTCC CCAGGTGCTA ACCAGGTGAC GAGCACGGTT 
GACAGCCTCG ACAGTCATGA CCTGGAAGGG GTGCAGATCG ATTTTGACGC CATCATAGAC 
GACGGGGACC ACGCCAGCCT GATGTCAGGA GCCCTGAGTC CCAGTATCAT CCAGAACCTC 
TCCCATAGCT CCTCCCGCCT CACGACACCC CGCGCGTCCC TCCCGCTGCC CACGCTGTCC 
GTGAGCACCA CCAACATGGC CATCGGGGAC ATGAGCTCCC TGCTGACCTC CCTGGCGGAA 
GAAAGCAAAT TCCTTGCAGT GATGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_022405177.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022260885.1 Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

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CloneID	OCe294660
Clone ID Related Accession (Same CDS sequence)	XM_022405177.1
Accession Version	XM_022405177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transcriptional activator GLI3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAG ACCGAAGAAA AATGATGGGA GAATATTCTA CTAGGTATAG ACTGCCAACC 
TGTTACCCAG CTCCGTGCAG GACCACAGAT CTAGTCATTC CATGGCTGCA TCCTAGAGAT 
TGTCAAGAGG ATGAAAGTCC TGGACAGACC TATCACAGAG AGAGAAGAAA CGCAGTCACT 
ATGCAGCCAC AGAGCGTCCA GGGGCTCGGC AAGATCAGCG AGGAGCCTTC GACGTCTAGC 
GAGGAGAGGG CCTCGCTGAT CAAGAAGGAG ATCCATGGGT CCCTGCCGCA CCTGGCCGAG 
CCCTCGGTGC CGTACCGTGG GGCCGTGTTT GCCATGGACC CCCGGAACGG TTACATGGAG 
CCCCACTACC ACCCTTCTCA TCTCTTTCCT GCATTCCACC CTCCTGTACC AATCGATGCA 
AGACATCATG AGGGGCGTTA CCATTATGAC CCGTCTCCGA TTCCTCCATT GCATGTGCCT 
TCTGCTTTAT CCAGTAGCCC AACGTATTCG GACCTGCCCT TCATCAGGAT CTCCCCACAC 
CGGAACCCCG CTGCAGCCTC TGAACCCCCC TTCAGCCCTC CACACCCCTA CATCAACCCC 
TACATGGACT ACATCCGGTC CCTACACAGC CCGTCCCTCT CCATGATCTC GGCAGCTCGG 
GGGCTCAGCC CTACGGATGC CCCCCATGCT GGGGTCAGCC CAGCGGAATA CTATCATCAG 
ATGGCTCTGC TGGCTGGCCA GCGCAGCCCC TACGCAGACA TCATTCCCTC CGCTGCCACT 
GCCGGCGCTG GGGCCATCCA CATGGAATAT CTTCATGCCA TGGACAGCAC CAGATTCCCC 
AGCCCCAGGC TGTCGGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCGCTGTCC 
GATCACAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGCAGCAG CTCTTCAGCG AGCGGCTCCT ACGGCCACTT GTCTGCGAGT 
GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCTGCAC CCGTGTCTCT CCACATGCAT 
CAGCAAATCT TAAGCCGACA ACAGAGCCTA GGCTCGGCCT TTGGACACAG CCCTCCGCTC 
ATCCACCCTG CTCCAACTTT TCCAACGCAG AGGCCTATTC CTGGGATCCC TACGGTGCTC 
AACCCCGTCC AGGTCACTTC TGGCCCTTCT GAGTCCTCAC AGCAGAACAA GCCGACGAGT 
GAGTCCGCGG TGAGTAGCAC TGGTGACCTG ATGCACAACA AGCGGTCCAA GATCAAACCT 
GACGAAGACC TACCCAGCCC GGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC 
CTGGTAAAGG AAGAGGGTGA CAGAGACGAA AGCAAACAAG AGCCCGAAGT CATCTATGAG 
ACCAACTGCC ACTGGGAAGG TTGCACGCGG GAGTTTGACA CCCAGGAGCA GCTGGTGCAC 
CATATAAATA ATGACCATAT TCACGGCGAG AAGAAGGAGT TCGTGTGCCG GTGGATGGAT 
TGCTCGAGGG AGCAGAAGCC CTTCAAAGCC CAGTACATGC TGGTGGTGCA CATGAGGAGA 
CACACCGGGG AGAAACCTCA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCGAGA 
CTAGAAAACT TGAAAACGCA CTTGAGATCT CACACTGGAG AGAAACCGTA CGTCTGTGAG 
CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAGCA CCAAAACAGG 
ACGCATTCCA ATGAGAAACC CTATGTGTGC AAAATCCCAG GCTGCACCAA GCGCTACACA 
GACCCAAGTT CCCTCCGGAA ACATGTGAAG ACCGTGCATG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GTGGGGACAT ACATCCTCGG CCCCCCCCAC CCCGAGATTC GGGCAGCCAT 
TCACAGTCCA GGTCGCCTGG CCGGCAGACT CAGGGAGCCC TTGGGGAGCA GAAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAGCTGTCAA GGCCGAGAAG 
CCCATGCAGA CATCTCAGCC AAGCCCTGGT GGTGAGTCTT CATGCAGCAG CCAACAGTCC 
CCCATCAGCA ACTATTCCAA CAGTGGGCTC GAGCTTCCTC TGACCGACGG AGGTGGCGTG 
GGAGACCTCG GGACCATCGA CGAAACCCCA ATCATGGACT CGACCATTTC CACCGCAACC 
ACCGCCCTCG CCTTGCAAGC CAGGAGGAAC CCGGCAGGGG CCAAATGGAT GGAGCATGTG 
AAACTAGAAA GGCTCAAACA AGTGAATGGA ATGCTTCCAC GACTGAACCC CGTTCTTCCC 
CCCAAAGCCC CTGCAGTCTC TCCTCTCATA GGAAACGGCA CTCAGCCCAG CACCAGCTGC 
AGTTTGGGCG GGCCCATAAC CCTCCTCCCG AACAGGAGCG ACCTCTCCGG GGTGGACATC 
ACCATGCTGA ACATGCTCAA CCGGAGGGAC AGCAGCGCCA GCACCATCAG CTCCGCCTAC 
CTGAGCAGCC GCCGCTCCTC GGGCATCTCC CCCTGCTTTT CCAGCCGCCG GTCCAGCGAG 
GCGTCCCAGG CCGAGGGCCG GCCCCAGAAC GTGAGCGTGG CCGACTCCTA CGACCCCATC 
TCCACCGACG CGTCGCGCAG GTCCAGCGAG GCGAGCCAGG GCGACGGGCT GCCCAGCCTG 
CTCAGCCTCA CGCCGGCGCA GCAGTACCGC CTGAAGGCCA AGTACGCGGC GGCCACGGGC 
GGGCCGCCCC CCACCCCGCT GCCCAACATG GAGAGGATGA GCCTCAAGAC CAGGATGGCC 
CTGCTCGGGG ACGCCCGGGA GCCCGCGGGG GCCCTGCCGC CCGTGCCCCC TCCGCGGCGG 
TGCAGCGACG GCGGCGCCCA CGGCTACGGC CGCCGCCCCC TGCTGCCCCT GGACGCGCTG 
GGCAACGGCG TGCGCAGGGC CAGCGACCCG GTGCGGACGG CGTCCGACGG CCTCTCCCTG 
CCCCGCGTGC AGCGCTTCGG CAGCCTTCAC AGCTTCAACC CGCCCGGGCC GCCTCCCACC 
CTGGAGAAGC GGCACCTGGT GCTCCAGAAT TACACGCGGC CCGACGGCGG CCAGCCCCGC 
CACTTCCCGC CGTCCCCCTG TCCGCCGAGC ATCACCGAGA ACGTCACCCT GGAGGCCCTG 
ACCATGGACG CCGACGTGAG CCTCACCGAT GAGGACTTCC TGCCCGACGA CGTGGTGCAG 
TATCTGAATT CCCAGAACCC GGCCGGGTTC GAGCAGCCCT TCCAGAGCGC CGTCTCCGAG 
GACAGCAAAG CGCCCCACGG GCCCGGCGAC TTCGACGGCC CGGGGCTGCC GGACGGTCAC 
GCGGGCCACC CAGGCCACCC GGGCCACCCG GGCCCCGCCG GCCAGCCGTA CCGCCCGCTG 
GAGCAGCCGT GCCCCGAAGC CAGCAAAGCC GACCTGCCCA TTCAGTGGAA CGAAGTGAGC 
TCGGGCAGCG CCGACCTGTG CTCCTCCAAG CTCAAGTGTG GCCAGCGGCC GGCGGCGCAG 
CAGGCCCGGG CCTTCGGCTT CTACGGCAAC GTGGGCGCCC CCCCGCAGCC CGCCTTGCGG 
AGCGGGCCCG GCCCCGCCGG CGCCTATGCC CCCCTGGCCG AGCCCGGCCC GGCCTACGGC 
GGCCCCGAGC CCTTGGCGGG CCACGGCGCT GGAGCGGGCA GCCCCGGAGA CGCGTTCCAC 
GAACAGCCCT GCAAGGCCCC GCAGTTCGGG AGCTGCCTGG GCAGGCAGCC GGGCGCCCCC 
GGCCCCCTCG ACGGCGGCTG CGCTGCGGCG GCAAAGCTCA AGAGCAGCAC GGCGCCGGGG 
CCCGGGGGCC AGCCCGACTT TGGCCTGCTG CCGGGGCCCG GCGAGTCAGC GGGCGGCGCC 
GGGAGCGGCC TGGCCACCCA AGACCTGGCA GGACAGGGGT ACCTGGCCCA TCCGCTGCTC 
CACGACGGCG TGCCCCACCA AGGGGCGGGC CGCGCAGGCC AGCAGATGCT AGGGCAGGTT 
AGCGCTACCT CACACGTCAA CGTCTATCTG GGGCCCGAGG GCGGCCTGCC AGGGCCGCCG 
TGCGTCAGCG GCCAGCCCGC CGGCTTGGCG GCCACCCGGG GCTACCAGCC GTGTGCGGCC 
TACGGGGGCG GCAGGCGCCA GGCTGTGCCG AGGGGCAGCC TCGCTCTGCA GCAGGCACAG 
CTCAGTGACC CAAGTCCGAC CTGCAGGGTG AACGGCATCA AGGTGGAGAT GCAGGGGCAG 
CCCCATCAGC TCTGTTCGAA CACGCAGAGT TACTCTGGTC AGTTCTATGA CCAAACCGTT 
GGCTTCAGTC AGCAAGACAT GAAAACTGGT TCATTCTCCA TTTCAGAAGC CAGCTGCCTG 
CTACAGGGGG CCAGCGCCGA GAACTCTGAA TTACTTTCCC CAGGTGCTAA CCAGGTGACG 
AGCACGGTTG ACAGCCTCGA CAGTCATGAC CTGGAAGGGG TGCAGATCGA TTTTGACGCC 
ATCATAGACG ACGGGGACCA CGCCAGCCTG ATGTCAGGAG CCCTGAGTCC CAGTATCATC 
CAGAACCTCT CCCATAGCTC CTCCCGCCTC ACGACACCCC GCGCGTCCCT CCCGCTGCCC 
ACGCTGTCCG TGAGCACCAC CAACATGGCC ATCGGGGACA TGAGCTCCCT GCTGACCTCC 
CTGGCGGAAG AAAGCAAATT CCTTGCAGTG ATGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260885.1
CDS	110..4888
Translation	MGKDRRKMMGEYSTRYRLPTCYPAPCRTTDLVIPWLHPRDCQEDESPGQTYHRERRNAVTMQPQSVQGLGKISEEPSTSSEERASLIKKEIHGSLPHLAEPSVPYRGAVFAMDPRNGYMEPHYHPSHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASEPPFSPPHPYINPYMDYIRSLHSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVTSGPSESSQQNKPTSESAVSSTGDLMHNKRSKIKPDEDLPSPGPRGQQEQPEGTTLVKEEGDRDESKQEPEVIYETNCHWEGCTREFDTQEQLVHHINNDHIHGEKKEFVCRWMDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRQTQGALGEQKDLSNTTSKREECLQVKAVKAEKPMQTSQPSPGGESSCSSQQSPISNYSNSGLELPLTDGGGVGDLGTIDETPIMDSTISTATTALALQARRNPAGAKWMEHVKLERLKQVNGMLPRLNPVLPPKAPAVSPLIGNGTQPSTSCSLGGPITLLPNRSDLSGVDITMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDAREPAGALPPVPPPRRCSDGGAHGYGRRPLLPLDALGNGVRRASDPVRTASDGLSLPRVQRFGSLHSFNPPGPPPTLEKRHLVLQNYTRPDGGQPRHFPPSPCPPSITENVTLEALTMDADVSLTDEDFLPDDVVQYLNSQNPAGFEQPFQSAVSEDSKAPHGPGDFDGPGLPDGHAGHPGHPGHPGPAGQPYRPLEQPCPEASKADLPIQWNEVSSGSADLCSSKLKCGQRPAAQQARAFGFYGNVGAPPQPALRSGPGPAGAYAPLAEPGPAYGGPEPLAGHGAGAGSPGDAFHEQPCKAPQFGSCLGRQPGAPGPLDGGCAAAAKLKSSTAPGPGGQPDFGLLPGPGESAGGAGSGLATQDLAGQGYLAHPLLHDGVPHQGAGRAGQQMLGQVSATSHVNVYLGPEGGLPGPPCVSGQPAGLAATRGYQPCAAYGGGRRQAVPRGSLALQQAQLSDPSPTCRVNGIKVEMQGQPHQLCSNTQSYSGQFYDQTVGFSQQDMKTGSFSISEASCLLQGASAENSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHASLMSGALSPSIIQNLSHSSSRLTTPRASLPLPTLSVSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_022405177.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022405177.1

ORF Insert Sequence:

ATGGGAAAAG ACCGAAGAAA AATGATGGGA GAATATTCTA CTAGGTATAG ACTGCCAACC 
TGTTACCCAG CTCCGTGCAG GACCACAGAT CTAGTCATTC CATGGCTGCA TCCTAGAGAT 
TGTCAAGAGG ATGAAAGTCC TGGACAGACC TATCACAGAG AGAGAAGAAA CGCAGTCACT 
ATGCAGCCAC AGAGCGTCCA GGGGCTCGGC AAGATCAGCG AGGAGCCTTC GACGTCTAGC 
GAGGAGAGGG CCTCGCTGAT CAAGAAGGAG ATCCATGGGT CCCTGCCGCA CCTGGCCGAG 
CCCTCGGTGC CGTACCGTGG GGCCGTGTTT GCCATGGACC CCCGGAACGG TTACATGGAG 
CCCCACTACC ACCCTTCTCA TCTCTTTCCT GCATTCCACC CTCCTGTACC AATCGATGCA 
AGACATCATG AGGGGCGTTA CCATTATGAC CCGTCTCCGA TTCCTCCATT GCATGTGCCT 
TCTGCTTTAT CCAGTAGCCC AACGTATTCG GACCTGCCCT TCATCAGGAT CTCCCCACAC 
CGGAACCCCG CTGCAGCCTC TGAACCCCCC TTCAGCCCTC CACACCCCTA CATCAACCCC 
TACATGGACT ACATCCGGTC CCTACACAGC CCGTCCCTCT CCATGATCTC GGCAGCTCGG 
GGGCTCAGCC CTACGGATGC CCCCCATGCT GGGGTCAGCC CAGCGGAATA CTATCATCAG 
ATGGCTCTGC TGGCTGGCCA GCGCAGCCCC TACGCAGACA TCATTCCCTC CGCTGCCACT 
GCCGGCGCTG GGGCCATCCA CATGGAATAT CTTCATGCCA TGGACAGCAC CAGATTCCCC 
AGCCCCAGGC TGTCGGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCGCTGTCC 
GATCACAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGCAGCAG CTCTTCAGCG AGCGGCTCCT ACGGCCACTT GTCTGCGAGT 
GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCTGCAC CCGTGTCTCT CCACATGCAT 
CAGCAAATCT TAAGCCGACA ACAGAGCCTA GGCTCGGCCT TTGGACACAG CCCTCCGCTC 
ATCCACCCTG CTCCAACTTT TCCAACGCAG AGGCCTATTC CTGGGATCCC TACGGTGCTC 
AACCCCGTCC AGGTCACTTC TGGCCCTTCT GAGTCCTCAC AGCAGAACAA GCCGACGAGT 
GAGTCCGCGG TGAGTAGCAC TGGTGACCTG ATGCACAACA AGCGGTCCAA GATCAAACCT 
GACGAAGACC TACCCAGCCC GGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC 
CTGGTAAAGG AAGAGGGTGA CAGAGACGAA AGCAAACAAG AGCCCGAAGT CATCTATGAG 
ACCAACTGCC ACTGGGAAGG TTGCACGCGG GAGTTTGACA CCCAGGAGCA GCTGGTGCAC 
CATATAAATA ATGACCATAT TCACGGCGAG AAGAAGGAGT TCGTGTGCCG GTGGATGGAT 
TGCTCGAGGG AGCAGAAGCC CTTCAAAGCC CAGTACATGC TGGTGGTGCA CATGAGGAGA 
CACACCGGGG AGAAACCTCA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCGAGA 
CTAGAAAACT TGAAAACGCA CTTGAGATCT CACACTGGAG AGAAACCGTA CGTCTGTGAG 
CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAGCA CCAAAACAGG 
ACGCATTCCA ATGAGAAACC CTATGTGTGC AAAATCCCAG GCTGCACCAA GCGCTACACA 
GACCCAAGTT CCCTCCGGAA ACATGTGAAG ACCGTGCATG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GTGGGGACAT ACATCCTCGG CCCCCCCCAC CCCGAGATTC GGGCAGCCAT 
TCACAGTCCA GGTCGCCTGG CCGGCAGACT CAGGGAGCCC TTGGGGAGCA GAAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAGCTGTCAA GGCCGAGAAG 
CCCATGCAGA CATCTCAGCC AAGCCCTGGT GGTGAGTCTT CATGCAGCAG CCAACAGTCC 
CCCATCAGCA ACTATTCCAA CAGTGGGCTC GAGCTTCCTC TGACCGACGG AGGTGGCGTG 
GGAGACCTCG GGACCATCGA CGAAACCCCA ATCATGGACT CGACCATTTC CACCGCAACC 
ACCGCCCTCG CCTTGCAAGC CAGGAGGAAC CCGGCAGGGG CCAAATGGAT GGAGCATGTG 
AAACTAGAAA GGCTCAAACA AGTGAATGGA ATGCTTCCAC GACTGAACCC CGTTCTTCCC 
CCCAAAGCCC CTGCAGTCTC TCCTCTCATA GGAAACGGCA CTCAGCCCAG CACCAGCTGC 
AGTTTGGGCG GGCCCATAAC CCTCCTCCCG AACAGGAGCG ACCTCTCCGG GGTGGACATC 
ACCATGCTGA ACATGCTCAA CCGGAGGGAC AGCAGCGCCA GCACCATCAG CTCCGCCTAC 
CTGAGCAGCC GCCGCTCCTC GGGCATCTCC CCCTGCTTTT CCAGCCGCCG GTCCAGCGAG 
GCGTCCCAGG CCGAGGGCCG GCCCCAGAAC GTGAGCGTGG CCGACTCCTA CGACCCCATC 
TCCACCGACG CGTCGCGCAG GTCCAGCGAG GCGAGCCAGG GCGACGGGCT GCCCAGCCTG 
CTCAGCCTCA CGCCGGCGCA GCAGTACCGC CTGAAGGCCA AGTACGCGGC GGCCACGGGC 
GGGCCGCCCC CCACCCCGCT GCCCAACATG GAGAGGATGA GCCTCAAGAC CAGGATGGCC 
CTGCTCGGGG ACGCCCGGGA GCCCGCGGGG GCCCTGCCGC CCGTGCCCCC TCCGCGGCGG 
TGCAGCGACG GCGGCGCCCA CGGCTACGGC CGCCGCCCCC TGCTGCCCCT GGACGCGCTG 
GGCAACGGCG TGCGCAGGGC CAGCGACCCG GTGCGGACGG CGTCCGACGG CCTCTCCCTG 
CCCCGCGTGC AGCGCTTCGG CAGCCTTCAC AGCTTCAACC CGCCCGGGCC GCCTCCCACC 
CTGGAGAAGC GGCACCTGGT GCTCCAGAAT TACACGCGGC CCGACGGCGG CCAGCCCCGC 
CACTTCCCGC CGTCCCCCTG TCCGCCGAGC ATCACCGAGA ACGTCACCCT GGAGGCCCTG 
ACCATGGACG CCGACGTGAG CCTCACCGAT GAGGACTTCC TGCCCGACGA CGTGGTGCAG 
TATCTGAATT CCCAGAACCC GGCCGGGTTC GAGCAGCCCT TCCAGAGCGC CGTCTCCGAG 
GACAGCAAAG CGCCCCACGG GCCCGGCGAC TTCGACGGCC CGGGGCTGCC GGACGGTCAC 
GCGGGCCACC CAGGCCACCC GGGCCACCCG GGCCCCGCCG GCCAGCCGTA CCGCCCGCTG 
GAGCAGCCGT GCCCCGAAGC CAGCAAAGCC GACCTGCCCA TTCAGTGGAA CGAAGTGAGC 
TCGGGCAGCG CCGACCTGTG CTCCTCCAAG CTCAAGTGTG GCCAGCGGCC GGCGGCGCAG 
CAGGCCCGGG CCTTCGGCTT CTACGGCAAC GTGGGCGCCC CCCCGCAGCC CGCCTTGCGG 
AGCGGGCCCG GCCCCGCCGG CGCCTATGCC CCCCTGGCCG AGCCCGGCCC GGCCTACGGC 
GGCCCCGAGC CCTTGGCGGG CCACGGCGCT GGAGCGGGCA GCCCCGGAGA CGCGTTCCAC 
GAACAGCCCT GCAAGGCCCC GCAGTTCGGG AGCTGCCTGG GCAGGCAGCC GGGCGCCCCC 
GGCCCCCTCG ACGGCGGCTG CGCTGCGGCG GCAAAGCTCA AGAGCAGCAC GGCGCCGGGG 
CCCGGGGGCC AGCCCGACTT TGGCCTGCTG CCGGGGCCCG GCGAGTCAGC GGGCGGCGCC 
GGGAGCGGCC TGGCCACCCA AGACCTGGCA GGACAGGGGT ACCTGGCCCA TCCGCTGCTC 
CACGACGGCG TGCCCCACCA AGGGGCGGGC CGCGCAGGCC AGCAGATGCT AGGGCAGGTT 
AGCGCTACCT CACACGTCAA CGTCTATCTG GGGCCCGAGG GCGGCCTGCC AGGGCCGCCG 
TGCGTCAGCG GCCAGCCCGC CGGCTTGGCG GCCACCCGGG GCTACCAGCC GTGTGCGGCC 
TACGGGGGCG GCAGGCGCCA GGCTGTGCCG AGGGGCAGCC TCGCTCTGCA GCAGGCACAG 
CTCAGTGACC CAAGTCCGAC CTGCAGGGTG AACGGCATCA AGGTGGAGAT GCAGGGGCAG 
CCCCATCAGC TCTGTTCGAA CACGCAGAGT TACTCTGGTC AGTTCTATGA CCAAACCGTT 
GGCTTCAGTC AGCAAGACAT GAAAACTGGT TCATTCTCCA TTTCAGAAGC CAGCTGCCTG 
CTACAGGGGG CCAGCGCCGA GAACTCTGAA TTACTTTCCC CAGGTGCTAA CCAGGTGACG 
AGCACGGTTG ACAGCCTCGA CAGTCATGAC CTGGAAGGGG TGCAGATCGA TTTTGACGCC 
ATCATAGACG ACGGGGACCA CGCCAGCCTG ATGTCAGGAG CCCTGAGTCC CAGTATCATC 
CAGAACCTCT CCCATAGCTC CTCCCGCCTC ACGACACCCC GCGCGTCCCT CCCGCTGCCC 
ACGCTGTCCG TGAGCACCAC CAACATGGCC ATCGGGGACA TGAGCTCCCT GCTGACCTCC 
CTGGCGGAAG AAAGCAAATT CCTTGCAGTG ATGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_022405178.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022260886.1 Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

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CloneID	OCe294661
Clone ID Related Accession (Same CDS sequence)	XM_022405178.1
Accession Version	XM_022405178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transcriptional activator GLI3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAAG ACCGAAGAAA AATGATGGGA GAATATTCTA CTAGGTATAG ACTGCCAACC 
TGTTACCCAG CTCCGTGCAG GACCACAGAT CTAGTCATTC CATGGCTGCA TCCTAGAGAT 
TGTCAAGAGG ATGAAAGTCC TGGACAGACC TATCACAGAG AGAGAAGAAA CGCAGTCACT 
ATGCAGCCAC AGAGCGTCCA GGGGCTCGGC AAGATCAGCG AGGAGCCTTC GACGTCTAGC 
GAGGAGAGGG CCTCGCTGAT CAAGAAGGAG ATCCATGGGT CCCTGCCGCA CCTGGCCGAG 
CCCTCGGTGC CGTACCGTGG GGCCGTGTTT GCCATGGACC CCCGGAACGG TTACATGGAG 
CCCCACTACC ACCCTTCTCA TCTCTTTCCT GCATTCCACC CTCCTGTACC AATCGATGCA 
AGACATCATG AGGGGCGTTA CCATTATGAC CCGTCTCCGA TTCCTCCATT GCATGTGCCT 
TCTGCTTTAT CCAGTAGCCC AACGTATTCG GACCTGCCCT TCATCAGGAT CTCCCCACAC 
CGGAACCCCG CTGCAGCCTC TGAACCCCCC TTCAGCCCTC CACACCCCTA CATCAACCCC 
TACATGGACT ACATCCGGTC CCTACACAGC CCGTCCCTCT CCATGATCTC GGCAGCTCGG 
GGGCTCAGCC CTACGGATGC CCCCCATGCT GGGGTCAGCC CAGCGGAATA CTATCATCAG 
ATGGCTCTGC TGGCTGGCCA GCGCAGCCCC TACGCAGACA TCATTCCCTC CGCTGCCACT 
GCCGGCGCTG GGGCCATCCA CATGGAATAT CTTCATGCCA TGGACAGCAC CAGATTCCCC 
AGCCCCAGGC TGTCGGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCGCTGTCC 
GATCACAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGCAGCAG CTCTTCAGCG AGCGGCTCCT ACGGCCACTT GTCTGCGAGT 
GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCTGCAC CCGTGTCTCT CCACATGCAT 
CAGCAAATCT TAAGCCGACA ACAGAGCCTA GGCTCGGCCT TTGGACACAG CCCTCCGCTC 
ATCCACCCTG CTCCAACTTT TCCAACGCAG AGGCCTATTC CTGGGATCCC TACGGTGCTC 
AACCCCGTCC AGGTCACTTC TGGCCCTTCT GAGTCCTCAC AGCAGAACAA GCCGACGAGT 
GAGTCCGCGG TGAGTAGCAC TGGTGACCTG ATGCACAACA AGCGGTCCAA GATCAAACCT 
GACGAAGACC TACCCAGCCC GGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC 
CTGGTAAAGG AAGAGGGTGA CAGAGACGAA AGCAAACAAG AGCCCGAAGT CATCTATGAG 
ACCAACTGCC ACTGGGAAGG TTGCACGCGG GAGTTTGACA CCCAGGAGCA GCTGGTGCAC 
CATATAAATA ATGACCATAT TCACGGCGAG AAGAAGGAGT TCGTGTGCCG GTGGATGGAT 
TGCTCGAGGG AGCAGAAGCC CTTCAAAGCC CAGTACATGC TGGTGGTGCA CATGAGGAGA 
CACACCGGGG AGAAACCTCA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCGAGA 
CTAGAAAACT TGAAAACGCA CTTGAGATCT CACACTGGAG AGAAACCGTA CGTCTGTGAG 
CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAGCA CCAAAACAGG 
ACGCATTCCA ATGAGAAACC CTATGTGTGC AAAATCCCAG GCTGCACCAA GCGCTACACA 
GACCCAAGTT CCCTCCGGAA ACATGTGAAG ACCGTGCATG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GTGGGGACAT ACATCCTCGG CCCCCCCCAC CCCGAGATTC GGGCAGCCAT 
TCACAGTCCA GGTCGCCTGG CCGGCAGACT CAGGGAGCCC TTGGGGAGCA GAAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAGCTGTCAA GGCCGAGAAG 
CCCATGACAT CTCAGCCAAG CCCTGGTGGT GAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGACGGAGG TGGCGTGGGA 
GACCTCGGGA CCATCGACGA AACCCCAATC ATGGACTCGA CCATTTCCAC CGCAACCACC 
GCCCTCGCCT TGCAAGCCAG GAGGAACCCG GCAGGGGCCA AATGGATGGA GCATGTGAAA 
CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCGT TCTTCCCCCC 
AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACTC AGCCCAGCAC CAGCTGCAGT 
TTGGGCGGGC CCATAACCCT CCTCCCGAAC AGGAGCGACC TCTCCGGGGT GGACATCACC 
ATGCTGAACA TGCTCAACCG GAGGGACAGC AGCGCCAGCA CCATCAGCTC CGCCTACCTG 
AGCAGCCGCC GCTCCTCGGG CATCTCCCCC TGCTTTTCCA GCCGCCGGTC CAGCGAGGCG 
TCCCAGGCCG AGGGCCGGCC CCAGAACGTG AGCGTGGCCG ACTCCTACGA CCCCATCTCC 
ACCGACGCGT CGCGCAGGTC CAGCGAGGCG AGCCAGGGCG ACGGGCTGCC CAGCCTGCTC 
AGCCTCACGC CGGCGCAGCA GTACCGCCTG AAGGCCAAGT ACGCGGCGGC CACGGGCGGG 
CCGCCCCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCAG GATGGCCCTG 
CTCGGGGACG CCCGGGAGCC CGCGGGGGCC CTGCCGCCCG TGCCCCCTCC GCGGCGGTGC 
AGCGACGGCG GCGCCCACGG CTACGGCCGC CGCCCCCTGC TGCCCCTGGA CGCGCTGGGC 
AACGGCGTGC GCAGGGCCAG CGACCCGGTG CGGACGGCGT CCGACGGCCT CTCCCTGCCC 
CGCGTGCAGC GCTTCGGCAG CCTTCACAGC TTCAACCCGC CCGGGCCGCC TCCCACCCTG 
GAGAAGCGGC ACCTGGTGCT CCAGAATTAC ACGCGGCCCG ACGGCGGCCA GCCCCGCCAC 
TTCCCGCCGT CCCCCTGTCC GCCGAGCATC ACCGAGAACG TCACCCTGGA GGCCCTGACC 
ATGGACGCCG ACGTGAGCCT CACCGATGAG GACTTCCTGC CCGACGACGT GGTGCAGTAT 
CTGAATTCCC AGAACCCGGC CGGGTTCGAG CAGCCCTTCC AGAGCGCCGT CTCCGAGGAC 
AGCAAAGCGC CCCACGGGCC CGGCGACTTC GACGGCCCGG GGCTGCCGGA CGGTCACGCG 
GGCCACCCAG GCCACCCGGG CCACCCGGGC CCCGCCGGCC AGCCGTACCG CCCGCTGGAG 
CAGCCGTGCC CCGAAGCCAG CAAAGCCGAC CTGCCCATTC AGTGGAACGA AGTGAGCTCG 
GGCAGCGCCG ACCTGTGCTC CTCCAAGCTC AAGTGTGGCC AGCGGCCGGC GGCGCAGCAG 
GCCCGGGCCT TCGGCTTCTA CGGCAACGTG GGCGCCCCCC CGCAGCCCGC CTTGCGGAGC 
GGGCCCGGCC CCGCCGGCGC CTATGCCCCC CTGGCCGAGC CCGGCCCGGC CTACGGCGGC 
CCCGAGCCCT TGGCGGGCCA CGGCGCTGGA GCGGGCAGCC CCGGAGACGC GTTCCACGAA 
CAGCCCTGCA AGGCCCCGCA GTTCGGGAGC TGCCTGGGCA GGCAGCCGGG CGCCCCCGGC 
CCCCTCGACG GCGGCTGCGC TGCGGCGGCA AAGCTCAAGA GCAGCACGGC GCCGGGGCCC 
GGGGGCCAGC CCGACTTTGG CCTGCTGCCG GGGCCCGGCG AGTCAGCGGG CGGCGCCGGG 
AGCGGCCTGG CCACCCAAGA CCTGGCAGGA CAGGGGTACC TGGCCCATCC GCTGCTCCAC 
GACGGCGTGC CCCACCAAGG GGCGGGCCGC GCAGGCCAGC AGATGCTAGG GCAGGTTAGC 
GCTACCTCAC ACGTCAACGT CTATCTGGGG CCCGAGGGCG GCCTGCCAGG GCCGCCGTGC 
GTCAGCGGCC AGCCCGCCGG CTTGGCGGCC ACCCGGGGCT ACCAGCCGTG TGCGGCCTAC 
GGGGGCGGCA GGCGCCAGGC TGTGCCGAGG GGCAGCCTCG CTCTGCAGCA GGCACAGCTC 
AGTGACCCAA GTCCGACCTG CAGGGTGAAC GGCATCAAGG TGGAGATGCA GGGGCAGCCC 
CATCAGCTCT GTTCGAACAC GCAGAGTTAC TCTGGTCAGT TCTATGACCA AACCGTTGGC 
TTCAGTCAGC AAGACATGAA AACTGGTTCA TTCTCCATTT CAGAAGCCAG CTGCCTGCTA 
CAGGGGGCCA GCGCCGAGAA CTCTGAATTA CTTTCCCCAG GTGCTAACCA GGTGACGAGC 
ACGGTTGACA GCCTCGACAG TCATGACCTG GAAGGGGTGC AGATCGATTT TGACGCCATC 
ATAGACGACG GGGACCACGC CAGCCTGATG TCAGGAGCCC TGAGTCCCAG TATCATCCAG 
AACCTCTCCC ATAGCTCCTC CCGCCTCACG ACACCCCGCG CGTCCCTCCC GCTGCCCACG 
CTGTCCGTGA GCACCACCAA CATGGCCATC GGGGACATGA GCTCCCTGCT GACCTCCCTG 
GCGGAAGAAA GCAAATTCCT TGCAGTGATG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260886.1
CDS	110..4885
Translation	MGKDRRKMMGEYSTRYRLPTCYPAPCRTTDLVIPWLHPRDCQEDESPGQTYHRERRNAVTMQPQSVQGLGKISEEPSTSSEERASLIKKEIHGSLPHLAEPSVPYRGAVFAMDPRNGYMEPHYHPSHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASEPPFSPPHPYINPYMDYIRSLHSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVTSGPSESSQQNKPTSESAVSSTGDLMHNKRSKIKPDEDLPSPGPRGQQEQPEGTTLVKEEGDRDESKQEPEVIYETNCHWEGCTREFDTQEQLVHHINNDHIHGEKKEFVCRWMDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRQTQGALGEQKDLSNTTSKREECLQVKAVKAEKPMTSQPSPGGESSCSSQQSPISNYSNSGLELPLTDGGGVGDLGTIDETPIMDSTISTATTALALQARRNPAGAKWMEHVKLERLKQVNGMLPRLNPVLPPKAPAVSPLIGNGTQPSTSCSLGGPITLLPNRSDLSGVDITMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDAREPAGALPPVPPPRRCSDGGAHGYGRRPLLPLDALGNGVRRASDPVRTASDGLSLPRVQRFGSLHSFNPPGPPPTLEKRHLVLQNYTRPDGGQPRHFPPSPCPPSITENVTLEALTMDADVSLTDEDFLPDDVVQYLNSQNPAGFEQPFQSAVSEDSKAPHGPGDFDGPGLPDGHAGHPGHPGHPGPAGQPYRPLEQPCPEASKADLPIQWNEVSSGSADLCSSKLKCGQRPAAQQARAFGFYGNVGAPPQPALRSGPGPAGAYAPLAEPGPAYGGPEPLAGHGAGAGSPGDAFHEQPCKAPQFGSCLGRQPGAPGPLDGGCAAAAKLKSSTAPGPGGQPDFGLLPGPGESAGGAGSGLATQDLAGQGYLAHPLLHDGVPHQGAGRAGQQMLGQVSATSHVNVYLGPEGGLPGPPCVSGQPAGLAATRGYQPCAAYGGGRRQAVPRGSLALQQAQLSDPSPTCRVNGIKVEMQGQPHQLCSNTQSYSGQFYDQTVGFSQQDMKTGSFSISEASCLLQGASAENSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHASLMSGALSPSIIQNLSHSSSRLTTPRASLPLPTLSVSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_022405178.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022405178.1

ORF Insert Sequence:

ATGGGAAAAG ACCGAAGAAA AATGATGGGA GAATATTCTA CTAGGTATAG ACTGCCAACC 
TGTTACCCAG CTCCGTGCAG GACCACAGAT CTAGTCATTC CATGGCTGCA TCCTAGAGAT 
TGTCAAGAGG ATGAAAGTCC TGGACAGACC TATCACAGAG AGAGAAGAAA CGCAGTCACT 
ATGCAGCCAC AGAGCGTCCA GGGGCTCGGC AAGATCAGCG AGGAGCCTTC GACGTCTAGC 
GAGGAGAGGG CCTCGCTGAT CAAGAAGGAG ATCCATGGGT CCCTGCCGCA CCTGGCCGAG 
CCCTCGGTGC CGTACCGTGG GGCCGTGTTT GCCATGGACC CCCGGAACGG TTACATGGAG 
CCCCACTACC ACCCTTCTCA TCTCTTTCCT GCATTCCACC CTCCTGTACC AATCGATGCA 
AGACATCATG AGGGGCGTTA CCATTATGAC CCGTCTCCGA TTCCTCCATT GCATGTGCCT 
TCTGCTTTAT CCAGTAGCCC AACGTATTCG GACCTGCCCT TCATCAGGAT CTCCCCACAC 
CGGAACCCCG CTGCAGCCTC TGAACCCCCC TTCAGCCCTC CACACCCCTA CATCAACCCC 
TACATGGACT ACATCCGGTC CCTACACAGC CCGTCCCTCT CCATGATCTC GGCAGCTCGG 
GGGCTCAGCC CTACGGATGC CCCCCATGCT GGGGTCAGCC CAGCGGAATA CTATCATCAG 
ATGGCTCTGC TGGCTGGCCA GCGCAGCCCC TACGCAGACA TCATTCCCTC CGCTGCCACT 
GCCGGCGCTG GGGCCATCCA CATGGAATAT CTTCATGCCA TGGACAGCAC CAGATTCCCC 
AGCCCCAGGC TGTCGGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCGCTGTCC 
GATCACAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGCAGCAG CTCTTCAGCG AGCGGCTCCT ACGGCCACTT GTCTGCGAGT 
GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCTGCAC CCGTGTCTCT CCACATGCAT 
CAGCAAATCT TAAGCCGACA ACAGAGCCTA GGCTCGGCCT TTGGACACAG CCCTCCGCTC 
ATCCACCCTG CTCCAACTTT TCCAACGCAG AGGCCTATTC CTGGGATCCC TACGGTGCTC 
AACCCCGTCC AGGTCACTTC TGGCCCTTCT GAGTCCTCAC AGCAGAACAA GCCGACGAGT 
GAGTCCGCGG TGAGTAGCAC TGGTGACCTG ATGCACAACA AGCGGTCCAA GATCAAACCT 
GACGAAGACC TACCCAGCCC GGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC 
CTGGTAAAGG AAGAGGGTGA CAGAGACGAA AGCAAACAAG AGCCCGAAGT CATCTATGAG 
ACCAACTGCC ACTGGGAAGG TTGCACGCGG GAGTTTGACA CCCAGGAGCA GCTGGTGCAC 
CATATAAATA ATGACCATAT TCACGGCGAG AAGAAGGAGT TCGTGTGCCG GTGGATGGAT 
TGCTCGAGGG AGCAGAAGCC CTTCAAAGCC CAGTACATGC TGGTGGTGCA CATGAGGAGA 
CACACCGGGG AGAAACCTCA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCGAGA 
CTAGAAAACT TGAAAACGCA CTTGAGATCT CACACTGGAG AGAAACCGTA CGTCTGTGAG 
CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAGCA CCAAAACAGG 
ACGCATTCCA ATGAGAAACC CTATGTGTGC AAAATCCCAG GCTGCACCAA GCGCTACACA 
GACCCAAGTT CCCTCCGGAA ACATGTGAAG ACCGTGCATG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GTGGGGACAT ACATCCTCGG CCCCCCCCAC CCCGAGATTC GGGCAGCCAT 
TCACAGTCCA GGTCGCCTGG CCGGCAGACT CAGGGAGCCC TTGGGGAGCA GAAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAGCTGTCAA GGCCGAGAAG 
CCCATGACAT CTCAGCCAAG CCCTGGTGGT GAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGACGGAGG TGGCGTGGGA 
GACCTCGGGA CCATCGACGA AACCCCAATC ATGGACTCGA CCATTTCCAC CGCAACCACC 
GCCCTCGCCT TGCAAGCCAG GAGGAACCCG GCAGGGGCCA AATGGATGGA GCATGTGAAA 
CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCGT TCTTCCCCCC 
AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACTC AGCCCAGCAC CAGCTGCAGT 
TTGGGCGGGC CCATAACCCT CCTCCCGAAC AGGAGCGACC TCTCCGGGGT GGACATCACC 
ATGCTGAACA TGCTCAACCG GAGGGACAGC AGCGCCAGCA CCATCAGCTC CGCCTACCTG 
AGCAGCCGCC GCTCCTCGGG CATCTCCCCC TGCTTTTCCA GCCGCCGGTC CAGCGAGGCG 
TCCCAGGCCG AGGGCCGGCC CCAGAACGTG AGCGTGGCCG ACTCCTACGA CCCCATCTCC 
ACCGACGCGT CGCGCAGGTC CAGCGAGGCG AGCCAGGGCG ACGGGCTGCC CAGCCTGCTC 
AGCCTCACGC CGGCGCAGCA GTACCGCCTG AAGGCCAAGT ACGCGGCGGC CACGGGCGGG 
CCGCCCCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCAG GATGGCCCTG 
CTCGGGGACG CCCGGGAGCC CGCGGGGGCC CTGCCGCCCG TGCCCCCTCC GCGGCGGTGC 
AGCGACGGCG GCGCCCACGG CTACGGCCGC CGCCCCCTGC TGCCCCTGGA CGCGCTGGGC 
AACGGCGTGC GCAGGGCCAG CGACCCGGTG CGGACGGCGT CCGACGGCCT CTCCCTGCCC 
CGCGTGCAGC GCTTCGGCAG CCTTCACAGC TTCAACCCGC CCGGGCCGCC TCCCACCCTG 
GAGAAGCGGC ACCTGGTGCT CCAGAATTAC ACGCGGCCCG ACGGCGGCCA GCCCCGCCAC 
TTCCCGCCGT CCCCCTGTCC GCCGAGCATC ACCGAGAACG TCACCCTGGA GGCCCTGACC 
ATGGACGCCG ACGTGAGCCT CACCGATGAG GACTTCCTGC CCGACGACGT GGTGCAGTAT 
CTGAATTCCC AGAACCCGGC CGGGTTCGAG CAGCCCTTCC AGAGCGCCGT CTCCGAGGAC 
AGCAAAGCGC CCCACGGGCC CGGCGACTTC GACGGCCCGG GGCTGCCGGA CGGTCACGCG 
GGCCACCCAG GCCACCCGGG CCACCCGGGC CCCGCCGGCC AGCCGTACCG CCCGCTGGAG 
CAGCCGTGCC CCGAAGCCAG CAAAGCCGAC CTGCCCATTC AGTGGAACGA AGTGAGCTCG 
GGCAGCGCCG ACCTGTGCTC CTCCAAGCTC AAGTGTGGCC AGCGGCCGGC GGCGCAGCAG 
GCCCGGGCCT TCGGCTTCTA CGGCAACGTG GGCGCCCCCC CGCAGCCCGC CTTGCGGAGC 
GGGCCCGGCC CCGCCGGCGC CTATGCCCCC CTGGCCGAGC CCGGCCCGGC CTACGGCGGC 
CCCGAGCCCT TGGCGGGCCA CGGCGCTGGA GCGGGCAGCC CCGGAGACGC GTTCCACGAA 
CAGCCCTGCA AGGCCCCGCA GTTCGGGAGC TGCCTGGGCA GGCAGCCGGG CGCCCCCGGC 
CCCCTCGACG GCGGCTGCGC TGCGGCGGCA AAGCTCAAGA GCAGCACGGC GCCGGGGCCC 
GGGGGCCAGC CCGACTTTGG CCTGCTGCCG GGGCCCGGCG AGTCAGCGGG CGGCGCCGGG 
AGCGGCCTGG CCACCCAAGA CCTGGCAGGA CAGGGGTACC TGGCCCATCC GCTGCTCCAC 
GACGGCGTGC CCCACCAAGG GGCGGGCCGC GCAGGCCAGC AGATGCTAGG GCAGGTTAGC 
GCTACCTCAC ACGTCAACGT CTATCTGGGG CCCGAGGGCG GCCTGCCAGG GCCGCCGTGC 
GTCAGCGGCC AGCCCGCCGG CTTGGCGGCC ACCCGGGGCT ACCAGCCGTG TGCGGCCTAC 
GGGGGCGGCA GGCGCCAGGC TGTGCCGAGG GGCAGCCTCG CTCTGCAGCA GGCACAGCTC 
AGTGACCCAA GTCCGACCTG CAGGGTGAAC GGCATCAAGG TGGAGATGCA GGGGCAGCCC 
CATCAGCTCT GTTCGAACAC GCAGAGTTAC TCTGGTCAGT TCTATGACCA AACCGTTGGC 
TTCAGTCAGC AAGACATGAA AACTGGTTCA TTCTCCATTT CAGAAGCCAG CTGCCTGCTA 
CAGGGGGCCA GCGCCGAGAA CTCTGAATTA CTTTCCCCAG GTGCTAACCA GGTGACGAGC 
ACGGTTGACA GCCTCGACAG TCATGACCTG GAAGGGGTGC AGATCGATTT TGACGCCATC 
ATAGACGACG GGGACCACGC CAGCCTGATG TCAGGAGCCC TGAGTCCCAG TATCATCCAG 
AACCTCTCCC ATAGCTCCTC CCGCCTCACG ACACCCCGCG CGTCCCTCCC GCTGCCCACG 
CTGTCCGTGA GCACCACCAA CATGGCCATC GGGGACATGA GCTCCCTGCT GACCTCCCTG 
GCGGAAGAAA GCAAATTCCT TGCAGTGATG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_014120824.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013976299.1 Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

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CloneID	OCe41022
Clone ID Related Accession (Same CDS sequence)	XM_014120824.1
Accession Version	XM_014120824.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	transcriptional activator GLI3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726091.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC AGAACCACAG CTCCACGACC ACTGAGAAGA AGAAAGTGGA CACTTCCATA 
GTGAAATGCT CCTCGAGAAC AGATGTCAGC GAGAAAGCGG TGGCCTCCAG CACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG 
CAGCCACAGA GCGTCCAGGG GCTCGGCAAG ATCAGCGAGG AGCCTTCGAC GTCTAGCGAG 
GAGAGGGCCT CGCTGATCAA GAAGGAGATC CATGGGTCCC TGCCGCACCT GGCCGAGCCC 
TCGGTGCCGT ACCGTGGGGC CGTGTTTGCC ATGGACCCCC GGAACGGTTA CATGGAGCCC 
CACTACCACC CTTCTCATCT CTTTCCTGCA TTCCACCCTC CTGTACCAAT CGATGCAAGA 
CATCATGAGG GGCGTTACCA TTATGACCCG TCTCCGATTC CTCCATTGCA TGTGCCTTCT 
GCTTTATCCA GTAGCCCAAC GTATTCGGAC CTGCCCTTCA TCAGGATCTC CCCACACCGG 
AACCCCGCTG CAGCCTCTGA ACCCCCCTTC AGCCCTCCAC ACCCCTACAT CAACCCCTAC 
ATGGACTACA TCCGGTCCCT ACACAGCCCG TCCCTCTCCA TGATCTCGGC AGCTCGGGGG 
CTCAGCCCTA CGGATGCCCC CCATGCTGGG GTCAGCCCAG CGGAATACTA TCATCAGATG 
GCTCTGCTGG CTGGCCAGCG CAGCCCCTAC GCAGACATCA TTCCCTCCGC TGCCACTGCC 
GGCGCTGGGG CCATCCACAT GGAATATCTT CATGCCATGG ACAGCACCAG ATTCCCCAGC 
CCCAGGCTGT CGGCCCGGCC GAGCCGAAAA CGTACACTGT CCATATCACC GCTGTCCGAT 
CACAGCTTTG ACCTTCAGAC CATGATAAGG ACATCTCCCA ACTCCTTGGT CACGATTCTC 
AATAATTCCC GCAGCAGCTC TTCAGCGAGC GGCTCCTACG GCCACTTGTC TGCGAGTGCA 
ATCAGCCCTG CCCTGAGCTT CACCTACCCT TCTGCACCCG TGTCTCTCCA CATGCATCAG 
CAAATCTTAA GCCGACAACA GAGCCTAGGC TCGGCCTTTG GACACAGCCC TCCGCTCATC 
CACCCTGCTC CAACTTTTCC AACGCAGAGG CCTATTCCTG GGATCCCTAC GGTGCTCAAC 
CCCGTCCAGG TCACTTCTGG CCCTTCTGAG TCCTCACAGC AGAACAAGCC GACGAGTGAG 
TCCGCGGTGA GTAGCACTGG TGACCTGATG CACAACAAGC GGTCCAAGAT CAAACCTGAC 
GAAGACCTAC CCAGCCCGGG GCCACGGGGC CAGCAGGAAC AGCCAGAAGG AACAACCCTG 
GTAAAGGAAG AGGGTGACAG AGACGAAAGC AAACAAGAGC CCGAAGTCAT CTATGAGACC 
AACTGCCACT GGGAAGGTTG CACGCGGGAG TTTGACACCC AGGAGCAGCT GGTGCACCAT 
ATAAATAATG ACCATATTCA CGGCGAGAAG AAGGAGTTCG TGTGCCGGTG GATGGATTGC 
TCGAGGGAGC AGAAGCCCTT CAAAGCCCAG TACATGCTGG TGGTGCACAT GAGGAGACAC 
ACCGGGGAGA AACCTCACAA ATGCACTTTT GAAGGTTGTA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACGCACTT GAGATCTCAC ACTGGAGAGA AACCGTACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAGCACCA AAACAGGACG 
CATTCCAATG AGAAACCCTA TGTGTGCAAA ATCCCAGGCT GCACCAAGCG CTACACAGAC 
CCAAGTTCCC TCCGGAAACA TGTGAAGACC GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGTG GGGACATACA TCCTCGGCCC CCCCCACCCC GAGATTCGGG CAGCCATTCA 
CAGTCCAGGT CGCCTGGCCG GCAGACTCAG GGAGCCCTTG GGGAGCAGAA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAAGTGAAAG CTGTCAAGGC CGAGAAGCCC 
ATGCAGACAT CTCAGCCAAG CCCTGGTGGT GAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGACGGAGG TGGCGTGGGA 
GACCTCGGGA CCATCGACGA AACCCCAATC ATGGACTCGA CCATTTCCAC CGCAACCACC 
GCCCTCGCCT TGCAAGCCAG GAGGAACCCG GCAGGGGCCA AATGGATGGA GCATGTGAAA 
CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCGT TCTTCCCCCC 
AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACTC AGCCCAGCAC CAGCTGCAGT 
TTGGGCGGGC CCATAACCCT CCTCCCGAAC AGGAGCGACC TCTCCGGGGT GGACATCACC 
ATGCTGAACA TGCTCAACCG GAGGGACAGC AGCGCCAGCA CCATCAGCTC CGCCTACCTG 
AGCAGCCGCC GCTCCTCGGG CATCTCCCCC TGCTTTTCCA GCCGCCGGTC CAGCGAGGCG 
TCCCAGGCCG AGGGCCGGCC CCAGAACGTG AGCGTGGCCG ACTCCTACGA CCCCATCTCC 
ACCGACGCGT CGCGCAGGTC CAGCGAGGCG AGCCAGGGCG ACGGGCTGCC CAGCCTGCTC 
AGCCTCACGC CGGCGCAGCA GTACCGCCTG AAGGCCAAGT ACGCGGCGGC CACGGGCGGG 
CCGCCCCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCAG GATGGCCCTG 
CTCGGGGACG CCCGGGAGCC CGCGGGGGCC CTGCCGCCCG TGCCCCCTCC GCGGCGGTGC 
AGCGACGGCG GCGCCCACGG CTACGGCCGC CGCCCCCTGC TGCCCCTGGA CGCGCTGGGC 
AACGGCGTGC GCAGGGCCAG CGACCCGGTG CGGACGGCGT CCGACGGCCT CTCCCTGCCC 
CGCGTGCAGC GCTTCGGCAG CCTTCACAGC TTCAACCCGC CCGGGCCGCC TCCCACCCTG 
GAGAAGCGGC ACCTGGTGCT CCAGAATTAC ACGCGGCCCG ACGGCGGCCA GCCCCGCCAC 
TTCCCGCCGT CCCCCTGTCC GCCGAGCATC ACCGAGAACG TCACCCTGGA GGCCCTGACC 
ATGGACGCCG ACGTGAGCCT CACCGATGAG GACTTCCTGC CCGACGACGT GGTGCAGTAT 
CTGAATTCCC AGAACCCGGC CGGGTTCGAG CAGCCCTTCC AGAGCGCCGT CTCCGAGGAC 
AGCAAAGCGC CCCACGGGCC CGGCGACTTC GACGGCCCGG GGCTGCCGGA CGGTCACGCG 
GGCCACCCAG GCCACCCGGG CCACCCGGGC CCCGCCGGCC AGCCGTACCG CCCGCTGGAG 
CAGCCGTGCC CCGAAGCCAG CAAAGCCGAC CTGCCCATTC AGTGGAACGA AGTGAGCTCG 
GGCAGCGCCG ACCTGTGCTC CTCCAAGCTC AAGTGTGGCC AGCGGCCGGC GGCGCAGCAG 
GCCCGGGCCT TCGGCTTCTA CGGCAACGTG GGCGCCCCCC CGCAGCCCGC CTTGCGGAGC 
GGGCCCGGCC CCGCCGGCGC CTATGCCCCC CTGGCCGAGC CCGGCCCGGC CTACGGCGGC 
CCCGAGCCCT TGGCGGGCCA CGGCGCTGGA GCGGGCAGCC CCGGAGACGC GTTCCACGAA 
CAGCCCTGCA AGGCCCCGCA GTTCGGGAGC TGCCTGGGCA GGCAGCCGGG CGCCCCCGGC 
CCCCTCGACG GCGGCTGCGC TGCGGCGGCA AAGCTCAAGA GCAGCACGGC GCCGGGGCCC 
GGGGGCCAGC CCGACTTTGG CCTGCTGCCG GGGCCCGGCG AGTCAGCGGG CGGCGCCGGG 
AGCGGCCTGG CCACCCAAGA CCTGGCAGGA CAGGGGTACC TGGCCCATCC GCTGCTCCAC 
GACGGCGTGC CCCACCAAGG GGCGGGCCGC GCAGGCCAGC AGATGCTAGG GCAGGTTAGC 
GCTACCTCAC ACGTCAACGT CTATCTGGGG CCCGAGGGCG GCCTGCCAGG GCCGCCGTGC 
GTCAGCGGCC AGCCCGCCGG CTTGGCGGCC ACCCGGGGCT ACCAGCCGTG TGCGGCCTAC 
GGGGGCGGCA GGCGCCAGGC TGTGCCGAGG GGCAGCCTCG CTCTGCAGCA GGCACAGCTC 
AGTGACCCAA GTCCGACCTG CAGGGTGAAC GGCATCAAGG TGGAGATGCA GGGGCAGCCC 
CATCAGCTCT GTTCGAACAC GCAGAGTTAC TCTGGTCAGT TCTATGACCA AACCGTTGGC 
TTCAGTCAGC AAGACATGAA AACTGGTTCA TTCTCCATTT CAGAAGCCAG CTGCCTGCTA 
CAGGGGGCCA GCGCCGAGAA CTCTGAATTA CTTTCCCCAG GTGCTAACCA GGTGACGAGC 
ACGGTTGACA GCCTCGACAG TCATGACCTG GAAGGGGTGC AGATCGATTT TGACGCCATC 
ATAGACGACG GGGACCACGC CAGCCTGATG TCAGGAGCCC TGAGTCCCAG TATCATCCAG 
AACCTCTCCC ATAGCTCCTC CCGCCTCACG ACACCCCGCG CGTCCCTCCC GCTGCCCACG 
CTGTCCGTGA GCACCACCAA CATGGCCATC GGGGACATGA GCTCCCTGCT GACCTCCCTG 
GCGGAAGAAA GCAAATTCCT TGCAGTGATG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976299.1
CDS	119..4894
Translation	MEAQNHSSTTTEKKKVDTSIVKCSSRTDVSEKAVASSTTSNEDESPGQTYHRERRNAVTMQPQSVQGLGKISEEPSTSSEERASLIKKEIHGSLPHLAEPSVPYRGAVFAMDPRNGYMEPHYHPSHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASEPPFSPPHPYINPYMDYIRSLHSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVTSGPSESSQQNKPTSESAVSSTGDLMHNKRSKIKPDEDLPSPGPRGQQEQPEGTTLVKEEGDRDESKQEPEVIYETNCHWEGCTREFDTQEQLVHHINNDHIHGEKKEFVCRWMDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRQTQGALGEQKDLSNTTSKREECLQVKAVKAEKPMQTSQPSPGGESSCSSQQSPISNYSNSGLELPLTDGGGVGDLGTIDETPIMDSTISTATTALALQARRNPAGAKWMEHVKLERLKQVNGMLPRLNPVLPPKAPAVSPLIGNGTQPSTSCSLGGPITLLPNRSDLSGVDITMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDAREPAGALPPVPPPRRCSDGGAHGYGRRPLLPLDALGNGVRRASDPVRTASDGLSLPRVQRFGSLHSFNPPGPPPTLEKRHLVLQNYTRPDGGQPRHFPPSPCPPSITENVTLEALTMDADVSLTDEDFLPDDVVQYLNSQNPAGFEQPFQSAVSEDSKAPHGPGDFDGPGLPDGHAGHPGHPGHPGPAGQPYRPLEQPCPEASKADLPIQWNEVSSGSADLCSSKLKCGQRPAAQQARAFGFYGNVGAPPQPALRSGPGPAGAYAPLAEPGPAYGGPEPLAGHGAGAGSPGDAFHEQPCKAPQFGSCLGRQPGAPGPLDGGCAAAAKLKSSTAPGPGGQPDFGLLPGPGESAGGAGSGLATQDLAGQGYLAHPLLHDGVPHQGAGRAGQQMLGQVSATSHVNVYLGPEGGLPGPPCVSGQPAGLAATRGYQPCAAYGGGRRQAVPRGSLALQQAQLSDPSPTCRVNGIKVEMQGQPHQLCSNTQSYSGQFYDQTVGFSQQDMKTGSFSISEASCLLQGASAENSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHASLMSGALSPSIIQNLSHSSSRLTTPRASLPLPTLSVSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_014120824.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120824.1

ORF Insert Sequence:

ATGGAGGCCC AGAACCACAG CTCCACGACC ACTGAGAAGA AGAAAGTGGA CACTTCCATA 
GTGAAATGCT CCTCGAGAAC AGATGTCAGC GAGAAAGCGG TGGCCTCCAG CACCACTTCC 
AATGAGGATG AAAGTCCTGG ACAGACCTAT CACAGAGAGA GAAGAAACGC AGTCACTATG 
CAGCCACAGA GCGTCCAGGG GCTCGGCAAG ATCAGCGAGG AGCCTTCGAC GTCTAGCGAG 
GAGAGGGCCT CGCTGATCAA GAAGGAGATC CATGGGTCCC TGCCGCACCT GGCCGAGCCC 
TCGGTGCCGT ACCGTGGGGC CGTGTTTGCC ATGGACCCCC GGAACGGTTA CATGGAGCCC 
CACTACCACC CTTCTCATCT CTTTCCTGCA TTCCACCCTC CTGTACCAAT CGATGCAAGA 
CATCATGAGG GGCGTTACCA TTATGACCCG TCTCCGATTC CTCCATTGCA TGTGCCTTCT 
GCTTTATCCA GTAGCCCAAC GTATTCGGAC CTGCCCTTCA TCAGGATCTC CCCACACCGG 
AACCCCGCTG CAGCCTCTGA ACCCCCCTTC AGCCCTCCAC ACCCCTACAT CAACCCCTAC 
ATGGACTACA TCCGGTCCCT ACACAGCCCG TCCCTCTCCA TGATCTCGGC AGCTCGGGGG 
CTCAGCCCTA CGGATGCCCC CCATGCTGGG GTCAGCCCAG CGGAATACTA TCATCAGATG 
GCTCTGCTGG CTGGCCAGCG CAGCCCCTAC GCAGACATCA TTCCCTCCGC TGCCACTGCC 
GGCGCTGGGG CCATCCACAT GGAATATCTT CATGCCATGG ACAGCACCAG ATTCCCCAGC 
CCCAGGCTGT CGGCCCGGCC GAGCCGAAAA CGTACACTGT CCATATCACC GCTGTCCGAT 
CACAGCTTTG ACCTTCAGAC CATGATAAGG ACATCTCCCA ACTCCTTGGT CACGATTCTC 
AATAATTCCC GCAGCAGCTC TTCAGCGAGC GGCTCCTACG GCCACTTGTC TGCGAGTGCA 
ATCAGCCCTG CCCTGAGCTT CACCTACCCT TCTGCACCCG TGTCTCTCCA CATGCATCAG 
CAAATCTTAA GCCGACAACA GAGCCTAGGC TCGGCCTTTG GACACAGCCC TCCGCTCATC 
CACCCTGCTC CAACTTTTCC AACGCAGAGG CCTATTCCTG GGATCCCTAC GGTGCTCAAC 
CCCGTCCAGG TCACTTCTGG CCCTTCTGAG TCCTCACAGC AGAACAAGCC GACGAGTGAG 
TCCGCGGTGA GTAGCACTGG TGACCTGATG CACAACAAGC GGTCCAAGAT CAAACCTGAC 
GAAGACCTAC CCAGCCCGGG GCCACGGGGC CAGCAGGAAC AGCCAGAAGG AACAACCCTG 
GTAAAGGAAG AGGGTGACAG AGACGAAAGC AAACAAGAGC CCGAAGTCAT CTATGAGACC 
AACTGCCACT GGGAAGGTTG CACGCGGGAG TTTGACACCC AGGAGCAGCT GGTGCACCAT 
ATAAATAATG ACCATATTCA CGGCGAGAAG AAGGAGTTCG TGTGCCGGTG GATGGATTGC 
TCGAGGGAGC AGAAGCCCTT CAAAGCCCAG TACATGCTGG TGGTGCACAT GAGGAGACAC 
ACCGGGGAGA AACCTCACAA ATGCACTTTT GAAGGTTGTA CAAAGGCCTA CTCGAGACTA 
GAAAACTTGA AAACGCACTT GAGATCTCAC ACTGGAGAGA AACCGTACGT CTGTGAGCAC 
GAAGGTTGCA ACAAGGCTTT CTCAAATGCC TCTGATCGCG CCAAGCACCA AAACAGGACG 
CATTCCAATG AGAAACCCTA TGTGTGCAAA ATCCCAGGCT GCACCAAGCG CTACACAGAC 
CCAAGTTCCC TCCGGAAACA TGTGAAGACC GTGCATGGCC CAGAGGCTCA TGTCACCAAG 
AAGCAGCGTG GGGACATACA TCCTCGGCCC CCCCCACCCC GAGATTCGGG CAGCCATTCA 
CAGTCCAGGT CGCCTGGCCG GCAGACTCAG GGAGCCCTTG GGGAGCAGAA GGACCTCAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAAGTGAAAG CTGTCAAGGC CGAGAAGCCC 
ATGCAGACAT CTCAGCCAAG CCCTGGTGGT GAGTCTTCAT GCAGCAGCCA ACAGTCCCCC 
ATCAGCAACT ATTCCAACAG TGGGCTCGAG CTTCCTCTGA CCGACGGAGG TGGCGTGGGA 
GACCTCGGGA CCATCGACGA AACCCCAATC ATGGACTCGA CCATTTCCAC CGCAACCACC 
GCCCTCGCCT TGCAAGCCAG GAGGAACCCG GCAGGGGCCA AATGGATGGA GCATGTGAAA 
CTAGAAAGGC TCAAACAAGT GAATGGAATG CTTCCACGAC TGAACCCCGT TCTTCCCCCC 
AAAGCCCCTG CAGTCTCTCC TCTCATAGGA AACGGCACTC AGCCCAGCAC CAGCTGCAGT 
TTGGGCGGGC CCATAACCCT CCTCCCGAAC AGGAGCGACC TCTCCGGGGT GGACATCACC 
ATGCTGAACA TGCTCAACCG GAGGGACAGC AGCGCCAGCA CCATCAGCTC CGCCTACCTG 
AGCAGCCGCC GCTCCTCGGG CATCTCCCCC TGCTTTTCCA GCCGCCGGTC CAGCGAGGCG 
TCCCAGGCCG AGGGCCGGCC CCAGAACGTG AGCGTGGCCG ACTCCTACGA CCCCATCTCC 
ACCGACGCGT CGCGCAGGTC CAGCGAGGCG AGCCAGGGCG ACGGGCTGCC CAGCCTGCTC 
AGCCTCACGC CGGCGCAGCA GTACCGCCTG AAGGCCAAGT ACGCGGCGGC CACGGGCGGG 
CCGCCCCCCA CCCCGCTGCC CAACATGGAG AGGATGAGCC TCAAGACCAG GATGGCCCTG 
CTCGGGGACG CCCGGGAGCC CGCGGGGGCC CTGCCGCCCG TGCCCCCTCC GCGGCGGTGC 
AGCGACGGCG GCGCCCACGG CTACGGCCGC CGCCCCCTGC TGCCCCTGGA CGCGCTGGGC 
AACGGCGTGC GCAGGGCCAG CGACCCGGTG CGGACGGCGT CCGACGGCCT CTCCCTGCCC 
CGCGTGCAGC GCTTCGGCAG CCTTCACAGC TTCAACCCGC CCGGGCCGCC TCCCACCCTG 
GAGAAGCGGC ACCTGGTGCT CCAGAATTAC ACGCGGCCCG ACGGCGGCCA GCCCCGCCAC 
TTCCCGCCGT CCCCCTGTCC GCCGAGCATC ACCGAGAACG TCACCCTGGA GGCCCTGACC 
ATGGACGCCG ACGTGAGCCT CACCGATGAG GACTTCCTGC CCGACGACGT GGTGCAGTAT 
CTGAATTCCC AGAACCCGGC CGGGTTCGAG CAGCCCTTCC AGAGCGCCGT CTCCGAGGAC 
AGCAAAGCGC CCCACGGGCC CGGCGACTTC GACGGCCCGG GGCTGCCGGA CGGTCACGCG 
GGCCACCCAG GCCACCCGGG CCACCCGGGC CCCGCCGGCC AGCCGTACCG CCCGCTGGAG 
CAGCCGTGCC CCGAAGCCAG CAAAGCCGAC CTGCCCATTC AGTGGAACGA AGTGAGCTCG 
GGCAGCGCCG ACCTGTGCTC CTCCAAGCTC AAGTGTGGCC AGCGGCCGGC GGCGCAGCAG 
GCCCGGGCCT TCGGCTTCTA CGGCAACGTG GGCGCCCCCC CGCAGCCCGC CTTGCGGAGC 
GGGCCCGGCC CCGCCGGCGC CTATGCCCCC CTGGCCGAGC CCGGCCCGGC CTACGGCGGC 
CCCGAGCCCT TGGCGGGCCA CGGCGCTGGA GCGGGCAGCC CCGGAGACGC GTTCCACGAA 
CAGCCCTGCA AGGCCCCGCA GTTCGGGAGC TGCCTGGGCA GGCAGCCGGG CGCCCCCGGC 
CCCCTCGACG GCGGCTGCGC TGCGGCGGCA AAGCTCAAGA GCAGCACGGC GCCGGGGCCC 
GGGGGCCAGC CCGACTTTGG CCTGCTGCCG GGGCCCGGCG AGTCAGCGGG CGGCGCCGGG 
AGCGGCCTGG CCACCCAAGA CCTGGCAGGA CAGGGGTACC TGGCCCATCC GCTGCTCCAC 
GACGGCGTGC CCCACCAAGG GGCGGGCCGC GCAGGCCAGC AGATGCTAGG GCAGGTTAGC 
GCTACCTCAC ACGTCAACGT CTATCTGGGG CCCGAGGGCG GCCTGCCAGG GCCGCCGTGC 
GTCAGCGGCC AGCCCGCCGG CTTGGCGGCC ACCCGGGGCT ACCAGCCGTG TGCGGCCTAC 
GGGGGCGGCA GGCGCCAGGC TGTGCCGAGG GGCAGCCTCG CTCTGCAGCA GGCACAGCTC 
AGTGACCCAA GTCCGACCTG CAGGGTGAAC GGCATCAAGG TGGAGATGCA GGGGCAGCCC 
CATCAGCTCT GTTCGAACAC GCAGAGTTAC TCTGGTCAGT TCTATGACCA AACCGTTGGC 
TTCAGTCAGC AAGACATGAA AACTGGTTCA TTCTCCATTT CAGAAGCCAG CTGCCTGCTA 
CAGGGGGCCA GCGCCGAGAA CTCTGAATTA CTTTCCCCAG GTGCTAACCA GGTGACGAGC 
ACGGTTGACA GCCTCGACAG TCATGACCTG GAAGGGGTGC AGATCGATTT TGACGCCATC 
ATAGACGACG GGGACCACGC CAGCCTGATG TCAGGAGCCC TGAGTCCCAG TATCATCCAG 
AACCTCTCCC ATAGCTCCTC CCGCCTCACG ACACCCCGCG CGTCCCTCCC GCTGCCCACG 
CTGTCCGTGA GCACCACCAA CATGGCCATC GGGGACATGA GCTCCCTGCT GACCTCCCTG 
GCGGAAGAAA GCAAATTCCT TGCAGTGATG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_014120825.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013976300.1 Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA.

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CloneID	OCe41023
Clone ID Related Accession (Same CDS sequence)	XM_014120825.2 , XM_014120825.1
Accession Version	XM_014120825.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transcriptional activator GLI3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120825.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCAC AGAGCGTCCA GGGGCTCGGC AAGATCAGCG AGGAGCCTTC GACGTCTAGC 
GAGGAGAGGG CCTCGCTGAT CAAGAAGGAG ATCCATGGGT CCCTGCCGCA CCTGGCCGAG 
CCCTCGGTGC CGTACCGTGG GGCCGTGTTT GCCATGGACC CCCGGAACGG TTACATGGAG 
CCCCACTACC ACCCTTCTCA TCTCTTTCCT GCATTCCACC CTCCTGTACC AATCGATGCA 
AGACATCATG AGGGGCGTTA CCATTATGAC CCGTCTCCGA TTCCTCCATT GCATGTGCCT 
TCTGCTTTAT CCAGTAGCCC AACGTATTCG GACCTGCCCT TCATCAGGAT CTCCCCACAC 
CGGAACCCCG CTGCAGCCTC TGAACCCCCC TTCAGCCCTC CACACCCCTA CATCAACCCC 
TACATGGACT ACATCCGGTC CCTACACAGC CCGTCCCTCT CCATGATCTC GGCAGCTCGG 
GGGCTCAGCC CTACGGATGC CCCCCATGCT GGGGTCAGCC CAGCGGAATA CTATCATCAG 
ATGGCTCTGC TGGCTGGCCA GCGCAGCCCC TACGCAGACA TCATTCCCTC CGCTGCCACT 
GCCGGCGCTG GGGCCATCCA CATGGAATAT CTTCATGCCA TGGACAGCAC CAGATTCCCC 
AGCCCCAGGC TGTCGGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCGCTGTCC 
GATCACAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGCAGCAG CTCTTCAGCG AGCGGCTCCT ACGGCCACTT GTCTGCGAGT 
GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCTGCAC CCGTGTCTCT CCACATGCAT 
CAGCAAATCT TAAGCCGACA ACAGAGCCTA GGCTCGGCCT TTGGACACAG CCCTCCGCTC 
ATCCACCCTG CTCCAACTTT TCCAACGCAG AGGCCTATTC CTGGGATCCC TACGGTGCTC 
AACCCCGTCC AGGTCACTTC TGGCCCTTCT GAGTCCTCAC AGCAGAACAA GCCGACGAGT 
GAGTCCGCGG TGAGTAGCAC TGGTGACCTG ATGCACAACA AGCGGTCCAA GATCAAACCT 
GACGAAGACC TACCCAGCCC GGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC 
CTGGTAAAGG AAGAGGGTGA CAGAGACGAA AGCAAACAAG AGCCCGAAGT CATCTATGAG 
ACCAACTGCC ACTGGGAAGG TTGCACGCGG GAGTTTGACA CCCAGGAGCA GCTGGTGCAC 
CATATAAATA ATGACCATAT TCACGGCGAG AAGAAGGAGT TCGTGTGCCG GTGGATGGAT 
TGCTCGAGGG AGCAGAAGCC CTTCAAAGCC CAGTACATGC TGGTGGTGCA CATGAGGAGA 
CACACCGGGG AGAAACCTCA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCGAGA 
CTAGAAAACT TGAAAACGCA CTTGAGATCT CACACTGGAG AGAAACCGTA CGTCTGTGAG 
CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAGCA CCAAAACAGG 
ACGCATTCCA ATGAGAAACC CTATGTGTGC AAAATCCCAG GCTGCACCAA GCGCTACACA 
GACCCAAGTT CCCTCCGGAA ACATGTGAAG ACCGTGCATG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GTGGGGACAT ACATCCTCGG CCCCCCCCAC CCCGAGATTC GGGCAGCCAT 
TCACAGTCCA GGTCGCCTGG CCGGCAGACT CAGGGAGCCC TTGGGGAGCA GAAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAGCTGTCAA GGCCGAGAAG 
CCCATGCAGA CATCTCAGCC AAGCCCTGGT GGTGAGTCTT CATGCAGCAG CCAACAGTCC 
CCCATCAGCA ACTATTCCAA CAGTGGGCTC GAGCTTCCTC TGACCGACGG AGGTGGCGTG 
GGAGACCTCG GGACCATCGA CGAAACCCCA ATCATGGACT CGACCATTTC CACCGCAACC 
ACCGCCCTCG CCTTGCAAGC CAGGAGGAAC CCGGCAGGGG CCAAATGGAT GGAGCATGTG 
AAACTAGAAA GGCTCAAACA AGTGAATGGA ATGCTTCCAC GACTGAACCC CGTTCTTCCC 
CCCAAAGCCC CTGCAGTCTC TCCTCTCATA GGAAACGGCA CTCAGCCCAG CACCAGCTGC 
AGTTTGGGCG GGCCCATAAC CCTCCTCCCG AACAGGAGCG ACCTCTCCGG GGTGGACATC 
ACCATGCTGA ACATGCTCAA CCGGAGGGAC AGCAGCGCCA GCACCATCAG CTCCGCCTAC 
CTGAGCAGCC GCCGCTCCTC GGGCATCTCC CCCTGCTTTT CCAGCCGCCG GTCCAGCGAG 
GCGTCCCAGG CCGAGGGCCG GCCCCAGAAC GTGAGCGTGG CCGACTCCTA CGACCCCATC 
TCCACCGACG CGTCGCGCAG GTCCAGCGAG GCGAGCCAGG GCGACGGGCT GCCCAGCCTG 
CTCAGCCTCA CGCCGGCGCA GCAGTACCGC CTGAAGGCCA AGTACGCGGC GGCCACGGGC 
GGGCCGCCCC CCACCCCGCT GCCCAACATG GAGAGGATGA GCCTCAAGAC CAGGATGGCC 
CTGCTCGGGG ACGCCCGGGA GCCCGCGGGG GCCCTGCCGC CCGTGCCCCC TCCGCGGCGG 
TGCAGCGACG GCGGCGCCCA CGGCTACGGC CGCCGCCCCC TGCTGCCCCT GGACGCGCTG 
GGCAACGGCG TGCGCAGGGC CAGCGACCCG GTGCGGACGG CGTCCGACGG CCTCTCCCTG 
CCCCGCGTGC AGCGCTTCGG CAGCCTTCAC AGCTTCAACC CGCCCGGGCC GCCTCCCACC 
CTGGAGAAGC GGCACCTGGT GCTCCAGAAT TACACGCGGC CCGACGGCGG CCAGCCCCGC 
CACTTCCCGC CGTCCCCCTG TCCGCCGAGC ATCACCGAGA ACGTCACCCT GGAGGCCCTG 
ACCATGGACG CCGACGTGAG CCTCACCGAT GAGGACTTCC TGCCCGACGA CGTGGTGCAG 
TATCTGAATT CCCAGAACCC GGCCGGGTTC GAGCAGCCCT TCCAGAGCGC CGTCTCCGAG 
GACAGCAAAG CGCCCCACGG GCCCGGCGAC TTCGACGGCC CGGGGCTGCC GGACGGTCAC 
GCGGGCCACC CAGGCCACCC GGGCCACCCG GGCCCCGCCG GCCAGCCGTA CCGCCCGCTG 
GAGCAGCCGT GCCCCGAAGC CAGCAAAGCC GACCTGCCCA TTCAGTGGAA CGAAGTGAGC 
TCGGGCAGCG CCGACCTGTG CTCCTCCAAG CTCAAGTGTG GCCAGCGGCC GGCGGCGCAG 
CAGGCCCGGG CCTTCGGCTT CTACGGCAAC GTGGGCGCCC CCCCGCAGCC CGCCTTGCGG 
AGCGGGCCCG GCCCCGCCGG CGCCTATGCC CCCCTGGCCG AGCCCGGCCC GGCCTACGGC 
GGCCCCGAGC CCTTGGCGGG CCACGGCGCT GGAGCGGGCA GCCCCGGAGA CGCGTTCCAC 
GAACAGCCCT GCAAGGCCCC GCAGTTCGGG AGCTGCCTGG GCAGGCAGCC GGGCGCCCCC 
GGCCCCCTCG ACGGCGGCTG CGCTGCGGCG GCAAAGCTCA AGAGCAGCAC GGCGCCGGGG 
CCCGGGGGCC AGCCCGACTT TGGCCTGCTG CCGGGGCCCG GCGAGTCAGC GGGCGGCGCC 
GGGAGCGGCC TGGCCACCCA AGACCTGGCA GGACAGGGGT ACCTGGCCCA TCCGCTGCTC 
CACGACGGCG TGCCCCACCA AGGGGCGGGC CGCGCAGGCC AGCAGATGCT AGGGCAGGTT 
AGCGCTACCT CACACGTCAA CGTCTATCTG GGGCCCGAGG GCGGCCTGCC AGGGCCGCCG 
TGCGTCAGCG GCCAGCCCGC CGGCTTGGCG GCCACCCGGG GCTACCAGCC GTGTGCGGCC 
TACGGGGGCG GCAGGCGCCA GGCTGTGCCG AGGGGCAGCC TCGCTCTGCA GCAGGCACAG 
CTCAGTGACC CAAGTCCGAC CTGCAGGGTG AACGGCATCA AGGTGGAGAT GCAGGGGCAG 
CCCCATCAGC TCTGTTCGAA CACGCAGAGT TACTCTGGTC AGTTCTATGA CCAAACCGTT 
GGCTTCAGTC AGCAAGACAT GAAAACTGGT TCATTCTCCA TTTCAGAAGC CAGCTGCCTG 
CTACAGGGGG CCAGCGCCGA GAACTCTGAA TTACTTTCCC CAGGTGCTAA CCAGGTGACG 
AGCACGGTTG ACAGCCTCGA CAGTCATGAC CTGGAAGGGG TGCAGATCGA TTTTGACGCC 
ATCATAGACG ACGGGGACCA CGCCAGCCTG ATGTCAGGAG CCCTGAGTCC CAGTATCATC 
CAGAACCTCT CCCATAGCTC CTCCCGCCTC ACGACACCCC GCGCGTCCCT CCCGCTGCCC 
ACGCTGTCCG TGAGCACCAC CAACATGGCC ATCGGGGACA TGAGCTCCCT GCTGACCTCC 
CTGGCGGAAG AAAGCAAATT CCTTGCAGTG ATGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976300.1
CDS	268..4866
Translation	MQPQSVQGLGKISEEPSTSSEERASLIKKEIHGSLPHLAEPSVPYRGAVFAMDPRNGYMEPHYHPSHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASEPPFSPPHPYINPYMDYIRSLHSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVTSGPSESSQQNKPTSESAVSSTGDLMHNKRSKIKPDEDLPSPGPRGQQEQPEGTTLVKEEGDRDESKQEPEVIYETNCHWEGCTREFDTQEQLVHHINNDHIHGEKKEFVCRWMDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRQTQGALGEQKDLSNTTSKREECLQVKAVKAEKPMQTSQPSPGGESSCSSQQSPISNYSNSGLELPLTDGGGVGDLGTIDETPIMDSTISTATTALALQARRNPAGAKWMEHVKLERLKQVNGMLPRLNPVLPPKAPAVSPLIGNGTQPSTSCSLGGPITLLPNRSDLSGVDITMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDAREPAGALPPVPPPRRCSDGGAHGYGRRPLLPLDALGNGVRRASDPVRTASDGLSLPRVQRFGSLHSFNPPGPPPTLEKRHLVLQNYTRPDGGQPRHFPPSPCPPSITENVTLEALTMDADVSLTDEDFLPDDVVQYLNSQNPAGFEQPFQSAVSEDSKAPHGPGDFDGPGLPDGHAGHPGHPGHPGPAGQPYRPLEQPCPEASKADLPIQWNEVSSGSADLCSSKLKCGQRPAAQQARAFGFYGNVGAPPQPALRSGPGPAGAYAPLAEPGPAYGGPEPLAGHGAGAGSPGDAFHEQPCKAPQFGSCLGRQPGAPGPLDGGCAAAAKLKSSTAPGPGGQPDFGLLPGPGESAGGAGSGLATQDLAGQGYLAHPLLHDGVPHQGAGRAGQQMLGQVSATSHVNVYLGPEGGLPGPPCVSGQPAGLAATRGYQPCAAYGGGRRQAVPRGSLALQQAQLSDPSPTCRVNGIKVEMQGQPHQLCSNTQSYSGQFYDQTVGFSQQDMKTGSFSISEASCLLQGASAENSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHASLMSGALSPSIIQNLSHSSSRLTTPRASLPLPTLSVSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_014120825.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120825.2

ORF Insert Sequence:

ATGCAGCCAC AGAGCGTCCA GGGGCTCGGC AAGATCAGCG AGGAGCCTTC GACGTCTAGC 
GAGGAGAGGG CCTCGCTGAT CAAGAAGGAG ATCCATGGGT CCCTGCCGCA CCTGGCCGAG 
CCCTCGGTGC CGTACCGTGG GGCCGTGTTT GCCATGGACC CCCGGAACGG TTACATGGAG 
CCCCACTACC ACCCTTCTCA TCTCTTTCCT GCATTCCACC CTCCTGTACC AATCGATGCA 
AGACATCATG AGGGGCGTTA CCATTATGAC CCGTCTCCGA TTCCTCCATT GCATGTGCCT 
TCTGCTTTAT CCAGTAGCCC AACGTATTCG GACCTGCCCT TCATCAGGAT CTCCCCACAC 
CGGAACCCCG CTGCAGCCTC TGAACCCCCC TTCAGCCCTC CACACCCCTA CATCAACCCC 
TACATGGACT ACATCCGGTC CCTACACAGC CCGTCCCTCT CCATGATCTC GGCAGCTCGG 
GGGCTCAGCC CTACGGATGC CCCCCATGCT GGGGTCAGCC CAGCGGAATA CTATCATCAG 
ATGGCTCTGC TGGCTGGCCA GCGCAGCCCC TACGCAGACA TCATTCCCTC CGCTGCCACT 
GCCGGCGCTG GGGCCATCCA CATGGAATAT CTTCATGCCA TGGACAGCAC CAGATTCCCC 
AGCCCCAGGC TGTCGGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCGCTGTCC 
GATCACAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGCAGCAG CTCTTCAGCG AGCGGCTCCT ACGGCCACTT GTCTGCGAGT 
GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCTGCAC CCGTGTCTCT CCACATGCAT 
CAGCAAATCT TAAGCCGACA ACAGAGCCTA GGCTCGGCCT TTGGACACAG CCCTCCGCTC 
ATCCACCCTG CTCCAACTTT TCCAACGCAG AGGCCTATTC CTGGGATCCC TACGGTGCTC 
AACCCCGTCC AGGTCACTTC TGGCCCTTCT GAGTCCTCAC AGCAGAACAA GCCGACGAGT 
GAGTCCGCGG TGAGTAGCAC TGGTGACCTG ATGCACAACA AGCGGTCCAA GATCAAACCT 
GACGAAGACC TACCCAGCCC GGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC 
CTGGTAAAGG AAGAGGGTGA CAGAGACGAA AGCAAACAAG AGCCCGAAGT CATCTATGAG 
ACCAACTGCC ACTGGGAAGG TTGCACGCGG GAGTTTGACA CCCAGGAGCA GCTGGTGCAC 
CATATAAATA ATGACCATAT TCACGGCGAG AAGAAGGAGT TCGTGTGCCG GTGGATGGAT 
TGCTCGAGGG AGCAGAAGCC CTTCAAAGCC CAGTACATGC TGGTGGTGCA CATGAGGAGA 
CACACCGGGG AGAAACCTCA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCGAGA 
CTAGAAAACT TGAAAACGCA CTTGAGATCT CACACTGGAG AGAAACCGTA CGTCTGTGAG 
CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAGCA CCAAAACAGG 
ACGCATTCCA ATGAGAAACC CTATGTGTGC AAAATCCCAG GCTGCACCAA GCGCTACACA 
GACCCAAGTT CCCTCCGGAA ACATGTGAAG ACCGTGCATG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GTGGGGACAT ACATCCTCGG CCCCCCCCAC CCCGAGATTC GGGCAGCCAT 
TCACAGTCCA GGTCGCCTGG CCGGCAGACT CAGGGAGCCC TTGGGGAGCA GAAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAGCTGTCAA GGCCGAGAAG 
CCCATGCAGA CATCTCAGCC AAGCCCTGGT GGTGAGTCTT CATGCAGCAG CCAACAGTCC 
CCCATCAGCA ACTATTCCAA CAGTGGGCTC GAGCTTCCTC TGACCGACGG AGGTGGCGTG 
GGAGACCTCG GGACCATCGA CGAAACCCCA ATCATGGACT CGACCATTTC CACCGCAACC 
ACCGCCCTCG CCTTGCAAGC CAGGAGGAAC CCGGCAGGGG CCAAATGGAT GGAGCATGTG 
AAACTAGAAA GGCTCAAACA AGTGAATGGA ATGCTTCCAC GACTGAACCC CGTTCTTCCC 
CCCAAAGCCC CTGCAGTCTC TCCTCTCATA GGAAACGGCA CTCAGCCCAG CACCAGCTGC 
AGTTTGGGCG GGCCCATAAC CCTCCTCCCG AACAGGAGCG ACCTCTCCGG GGTGGACATC 
ACCATGCTGA ACATGCTCAA CCGGAGGGAC AGCAGCGCCA GCACCATCAG CTCCGCCTAC 
CTGAGCAGCC GCCGCTCCTC GGGCATCTCC CCCTGCTTTT CCAGCCGCCG GTCCAGCGAG 
GCGTCCCAGG CCGAGGGCCG GCCCCAGAAC GTGAGCGTGG CCGACTCCTA CGACCCCATC 
TCCACCGACG CGTCGCGCAG GTCCAGCGAG GCGAGCCAGG GCGACGGGCT GCCCAGCCTG 
CTCAGCCTCA CGCCGGCGCA GCAGTACCGC CTGAAGGCCA AGTACGCGGC GGCCACGGGC 
GGGCCGCCCC CCACCCCGCT GCCCAACATG GAGAGGATGA GCCTCAAGAC CAGGATGGCC 
CTGCTCGGGG ACGCCCGGGA GCCCGCGGGG GCCCTGCCGC CCGTGCCCCC TCCGCGGCGG 
TGCAGCGACG GCGGCGCCCA CGGCTACGGC CGCCGCCCCC TGCTGCCCCT GGACGCGCTG 
GGCAACGGCG TGCGCAGGGC CAGCGACCCG GTGCGGACGG CGTCCGACGG CCTCTCCCTG 
CCCCGCGTGC AGCGCTTCGG CAGCCTTCAC AGCTTCAACC CGCCCGGGCC GCCTCCCACC 
CTGGAGAAGC GGCACCTGGT GCTCCAGAAT TACACGCGGC CCGACGGCGG CCAGCCCCGC 
CACTTCCCGC CGTCCCCCTG TCCGCCGAGC ATCACCGAGA ACGTCACCCT GGAGGCCCTG 
ACCATGGACG CCGACGTGAG CCTCACCGAT GAGGACTTCC TGCCCGACGA CGTGGTGCAG 
TATCTGAATT CCCAGAACCC GGCCGGGTTC GAGCAGCCCT TCCAGAGCGC CGTCTCCGAG 
GACAGCAAAG CGCCCCACGG GCCCGGCGAC TTCGACGGCC CGGGGCTGCC GGACGGTCAC 
GCGGGCCACC CAGGCCACCC GGGCCACCCG GGCCCCGCCG GCCAGCCGTA CCGCCCGCTG 
GAGCAGCCGT GCCCCGAAGC CAGCAAAGCC GACCTGCCCA TTCAGTGGAA CGAAGTGAGC 
TCGGGCAGCG CCGACCTGTG CTCCTCCAAG CTCAAGTGTG GCCAGCGGCC GGCGGCGCAG 
CAGGCCCGGG CCTTCGGCTT CTACGGCAAC GTGGGCGCCC CCCCGCAGCC CGCCTTGCGG 
AGCGGGCCCG GCCCCGCCGG CGCCTATGCC CCCCTGGCCG AGCCCGGCCC GGCCTACGGC 
GGCCCCGAGC CCTTGGCGGG CCACGGCGCT GGAGCGGGCA GCCCCGGAGA CGCGTTCCAC 
GAACAGCCCT GCAAGGCCCC GCAGTTCGGG AGCTGCCTGG GCAGGCAGCC GGGCGCCCCC 
GGCCCCCTCG ACGGCGGCTG CGCTGCGGCG GCAAAGCTCA AGAGCAGCAC GGCGCCGGGG 
CCCGGGGGCC AGCCCGACTT TGGCCTGCTG CCGGGGCCCG GCGAGTCAGC GGGCGGCGCC 
GGGAGCGGCC TGGCCACCCA AGACCTGGCA GGACAGGGGT ACCTGGCCCA TCCGCTGCTC 
CACGACGGCG TGCCCCACCA AGGGGCGGGC CGCGCAGGCC AGCAGATGCT AGGGCAGGTT 
AGCGCTACCT CACACGTCAA CGTCTATCTG GGGCCCGAGG GCGGCCTGCC AGGGCCGCCG 
TGCGTCAGCG GCCAGCCCGC CGGCTTGGCG GCCACCCGGG GCTACCAGCC GTGTGCGGCC 
TACGGGGGCG GCAGGCGCCA GGCTGTGCCG AGGGGCAGCC TCGCTCTGCA GCAGGCACAG 
CTCAGTGACC CAAGTCCGAC CTGCAGGGTG AACGGCATCA AGGTGGAGAT GCAGGGGCAG 
CCCCATCAGC TCTGTTCGAA CACGCAGAGT TACTCTGGTC AGTTCTATGA CCAAACCGTT 
GGCTTCAGTC AGCAAGACAT GAAAACTGGT TCATTCTCCA TTTCAGAAGC CAGCTGCCTG 
CTACAGGGGG CCAGCGCCGA GAACTCTGAA TTACTTTCCC CAGGTGCTAA CCAGGTGACG 
AGCACGGTTG ACAGCCTCGA CAGTCATGAC CTGGAAGGGG TGCAGATCGA TTTTGACGCC 
ATCATAGACG ACGGGGACCA CGCCAGCCTG ATGTCAGGAG CCCTGAGTCC CAGTATCATC 
CAGAACCTCT CCCATAGCTC CTCCCGCCTC ACGACACCCC GCGCGTCCCT CCCGCTGCCC 
ACGCTGTCCG TGAGCACCAC CAACATGGCC ATCGGGGACA TGAGCTCCCT GCTGACCTCC 
CTGGCGGAAG AAAGCAAATT CCTTGCAGTG ATGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLI3 ( XM_014120825.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013976300.1 Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.

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CloneID	OCe41023
Clone ID Related Accession (Same CDS sequence)	XM_014120825.2 , XM_014120825.1
Accession Version	XM_014120825.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	transcriptional activator GLI3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726091.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCAC AGAGCGTCCA GGGGCTCGGC AAGATCAGCG AGGAGCCTTC GACGTCTAGC 
GAGGAGAGGG CCTCGCTGAT CAAGAAGGAG ATCCATGGGT CCCTGCCGCA CCTGGCCGAG 
CCCTCGGTGC CGTACCGTGG GGCCGTGTTT GCCATGGACC CCCGGAACGG TTACATGGAG 
CCCCACTACC ACCCTTCTCA TCTCTTTCCT GCATTCCACC CTCCTGTACC AATCGATGCA 
AGACATCATG AGGGGCGTTA CCATTATGAC CCGTCTCCGA TTCCTCCATT GCATGTGCCT 
TCTGCTTTAT CCAGTAGCCC AACGTATTCG GACCTGCCCT TCATCAGGAT CTCCCCACAC 
CGGAACCCCG CTGCAGCCTC TGAACCCCCC TTCAGCCCTC CACACCCCTA CATCAACCCC 
TACATGGACT ACATCCGGTC CCTACACAGC CCGTCCCTCT CCATGATCTC GGCAGCTCGG 
GGGCTCAGCC CTACGGATGC CCCCCATGCT GGGGTCAGCC CAGCGGAATA CTATCATCAG 
ATGGCTCTGC TGGCTGGCCA GCGCAGCCCC TACGCAGACA TCATTCCCTC CGCTGCCACT 
GCCGGCGCTG GGGCCATCCA CATGGAATAT CTTCATGCCA TGGACAGCAC CAGATTCCCC 
AGCCCCAGGC TGTCGGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCGCTGTCC 
GATCACAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGCAGCAG CTCTTCAGCG AGCGGCTCCT ACGGCCACTT GTCTGCGAGT 
GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCTGCAC CCGTGTCTCT CCACATGCAT 
CAGCAAATCT TAAGCCGACA ACAGAGCCTA GGCTCGGCCT TTGGACACAG CCCTCCGCTC 
ATCCACCCTG CTCCAACTTT TCCAACGCAG AGGCCTATTC CTGGGATCCC TACGGTGCTC 
AACCCCGTCC AGGTCACTTC TGGCCCTTCT GAGTCCTCAC AGCAGAACAA GCCGACGAGT 
GAGTCCGCGG TGAGTAGCAC TGGTGACCTG ATGCACAACA AGCGGTCCAA GATCAAACCT 
GACGAAGACC TACCCAGCCC GGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC 
CTGGTAAAGG AAGAGGGTGA CAGAGACGAA AGCAAACAAG AGCCCGAAGT CATCTATGAG 
ACCAACTGCC ACTGGGAAGG TTGCACGCGG GAGTTTGACA CCCAGGAGCA GCTGGTGCAC 
CATATAAATA ATGACCATAT TCACGGCGAG AAGAAGGAGT TCGTGTGCCG GTGGATGGAT 
TGCTCGAGGG AGCAGAAGCC CTTCAAAGCC CAGTACATGC TGGTGGTGCA CATGAGGAGA 
CACACCGGGG AGAAACCTCA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCGAGA 
CTAGAAAACT TGAAAACGCA CTTGAGATCT CACACTGGAG AGAAACCGTA CGTCTGTGAG 
CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAGCA CCAAAACAGG 
ACGCATTCCA ATGAGAAACC CTATGTGTGC AAAATCCCAG GCTGCACCAA GCGCTACACA 
GACCCAAGTT CCCTCCGGAA ACATGTGAAG ACCGTGCATG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GTGGGGACAT ACATCCTCGG CCCCCCCCAC CCCGAGATTC GGGCAGCCAT 
TCACAGTCCA GGTCGCCTGG CCGGCAGACT CAGGGAGCCC TTGGGGAGCA GAAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAGCTGTCAA GGCCGAGAAG 
CCCATGCAGA CATCTCAGCC AAGCCCTGGT GGTGAGTCTT CATGCAGCAG CCAACAGTCC 
CCCATCAGCA ACTATTCCAA CAGTGGGCTC GAGCTTCCTC TGACCGACGG AGGTGGCGTG 
GGAGACCTCG GGACCATCGA CGAAACCCCA ATCATGGACT CGACCATTTC CACCGCAACC 
ACCGCCCTCG CCTTGCAAGC CAGGAGGAAC CCGGCAGGGG CCAAATGGAT GGAGCATGTG 
AAACTAGAAA GGCTCAAACA AGTGAATGGA ATGCTTCCAC GACTGAACCC CGTTCTTCCC 
CCCAAAGCCC CTGCAGTCTC TCCTCTCATA GGAAACGGCA CTCAGCCCAG CACCAGCTGC 
AGTTTGGGCG GGCCCATAAC CCTCCTCCCG AACAGGAGCG ACCTCTCCGG GGTGGACATC 
ACCATGCTGA ACATGCTCAA CCGGAGGGAC AGCAGCGCCA GCACCATCAG CTCCGCCTAC 
CTGAGCAGCC GCCGCTCCTC GGGCATCTCC CCCTGCTTTT CCAGCCGCCG GTCCAGCGAG 
GCGTCCCAGG CCGAGGGCCG GCCCCAGAAC GTGAGCGTGG CCGACTCCTA CGACCCCATC 
TCCACCGACG CGTCGCGCAG GTCCAGCGAG GCGAGCCAGG GCGACGGGCT GCCCAGCCTG 
CTCAGCCTCA CGCCGGCGCA GCAGTACCGC CTGAAGGCCA AGTACGCGGC GGCCACGGGC 
GGGCCGCCCC CCACCCCGCT GCCCAACATG GAGAGGATGA GCCTCAAGAC CAGGATGGCC 
CTGCTCGGGG ACGCCCGGGA GCCCGCGGGG GCCCTGCCGC CCGTGCCCCC TCCGCGGCGG 
TGCAGCGACG GCGGCGCCCA CGGCTACGGC CGCCGCCCCC TGCTGCCCCT GGACGCGCTG 
GGCAACGGCG TGCGCAGGGC CAGCGACCCG GTGCGGACGG CGTCCGACGG CCTCTCCCTG 
CCCCGCGTGC AGCGCTTCGG CAGCCTTCAC AGCTTCAACC CGCCCGGGCC GCCTCCCACC 
CTGGAGAAGC GGCACCTGGT GCTCCAGAAT TACACGCGGC CCGACGGCGG CCAGCCCCGC 
CACTTCCCGC CGTCCCCCTG TCCGCCGAGC ATCACCGAGA ACGTCACCCT GGAGGCCCTG 
ACCATGGACG CCGACGTGAG CCTCACCGAT GAGGACTTCC TGCCCGACGA CGTGGTGCAG 
TATCTGAATT CCCAGAACCC GGCCGGGTTC GAGCAGCCCT TCCAGAGCGC CGTCTCCGAG 
GACAGCAAAG CGCCCCACGG GCCCGGCGAC TTCGACGGCC CGGGGCTGCC GGACGGTCAC 
GCGGGCCACC CAGGCCACCC GGGCCACCCG GGCCCCGCCG GCCAGCCGTA CCGCCCGCTG 
GAGCAGCCGT GCCCCGAAGC CAGCAAAGCC GACCTGCCCA TTCAGTGGAA CGAAGTGAGC 
TCGGGCAGCG CCGACCTGTG CTCCTCCAAG CTCAAGTGTG GCCAGCGGCC GGCGGCGCAG 
CAGGCCCGGG CCTTCGGCTT CTACGGCAAC GTGGGCGCCC CCCCGCAGCC CGCCTTGCGG 
AGCGGGCCCG GCCCCGCCGG CGCCTATGCC CCCCTGGCCG AGCCCGGCCC GGCCTACGGC 
GGCCCCGAGC CCTTGGCGGG CCACGGCGCT GGAGCGGGCA GCCCCGGAGA CGCGTTCCAC 
GAACAGCCCT GCAAGGCCCC GCAGTTCGGG AGCTGCCTGG GCAGGCAGCC GGGCGCCCCC 
GGCCCCCTCG ACGGCGGCTG CGCTGCGGCG GCAAAGCTCA AGAGCAGCAC GGCGCCGGGG 
CCCGGGGGCC AGCCCGACTT TGGCCTGCTG CCGGGGCCCG GCGAGTCAGC GGGCGGCGCC 
GGGAGCGGCC TGGCCACCCA AGACCTGGCA GGACAGGGGT ACCTGGCCCA TCCGCTGCTC 
CACGACGGCG TGCCCCACCA AGGGGCGGGC CGCGCAGGCC AGCAGATGCT AGGGCAGGTT 
AGCGCTACCT CACACGTCAA CGTCTATCTG GGGCCCGAGG GCGGCCTGCC AGGGCCGCCG 
TGCGTCAGCG GCCAGCCCGC CGGCTTGGCG GCCACCCGGG GCTACCAGCC GTGTGCGGCC 
TACGGGGGCG GCAGGCGCCA GGCTGTGCCG AGGGGCAGCC TCGCTCTGCA GCAGGCACAG 
CTCAGTGACC CAAGTCCGAC CTGCAGGGTG AACGGCATCA AGGTGGAGAT GCAGGGGCAG 
CCCCATCAGC TCTGTTCGAA CACGCAGAGT TACTCTGGTC AGTTCTATGA CCAAACCGTT 
GGCTTCAGTC AGCAAGACAT GAAAACTGGT TCATTCTCCA TTTCAGAAGC CAGCTGCCTG 
CTACAGGGGG CCAGCGCCGA GAACTCTGAA TTACTTTCCC CAGGTGCTAA CCAGGTGACG 
AGCACGGTTG ACAGCCTCGA CAGTCATGAC CTGGAAGGGG TGCAGATCGA TTTTGACGCC 
ATCATAGACG ACGGGGACCA CGCCAGCCTG ATGTCAGGAG CCCTGAGTCC CAGTATCATC 
CAGAACCTCT CCCATAGCTC CTCCCGCCTC ACGACACCCC GCGCGTCCCT CCCGCTGCCC 
ACGCTGTCCG TGAGCACCAC CAACATGGCC ATCGGGGACA TGAGCTCCCT GCTGACCTCC 
CTGGCGGAAG AAAGCAAATT CCTTGCAGTG ATGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976300.1
CDS	271..4869
Translation	MQPQSVQGLGKISEEPSTSSEERASLIKKEIHGSLPHLAEPSVPYRGAVFAMDPRNGYMEPHYHPSHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYSDLPFIRISPHRNPAAASEPPFSPPHPYINPYMDYIRSLHSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLAGQRSPYADIIPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVTSGPSESSQQNKPTSESAVSSTGDLMHNKRSKIKPDEDLPSPGPRGQQEQPEGTTLVKEEGDRDESKQEPEVIYETNCHWEGCTREFDTQEQLVHHINNDHIHGEKKEFVCRWMDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDIHPRPPPPRDSGSHSQSRSPGRQTQGALGEQKDLSNTTSKREECLQVKAVKAEKPMQTSQPSPGGESSCSSQQSPISNYSNSGLELPLTDGGGVGDLGTIDETPIMDSTISTATTALALQARRNPAGAKWMEHVKLERLKQVNGMLPRLNPVLPPKAPAVSPLIGNGTQPSTSCSLGGPITLLPNRSDLSGVDITMLNMLNRRDSSASTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPAQQYRLKAKYAAATGGPPPTPLPNMERMSLKTRMALLGDAREPAGALPPVPPPRRCSDGGAHGYGRRPLLPLDALGNGVRRASDPVRTASDGLSLPRVQRFGSLHSFNPPGPPPTLEKRHLVLQNYTRPDGGQPRHFPPSPCPPSITENVTLEALTMDADVSLTDEDFLPDDVVQYLNSQNPAGFEQPFQSAVSEDSKAPHGPGDFDGPGLPDGHAGHPGHPGHPGPAGQPYRPLEQPCPEASKADLPIQWNEVSSGSADLCSSKLKCGQRPAAQQARAFGFYGNVGAPPQPALRSGPGPAGAYAPLAEPGPAYGGPEPLAGHGAGAGSPGDAFHEQPCKAPQFGSCLGRQPGAPGPLDGGCAAAAKLKSSTAPGPGGQPDFGLLPGPGESAGGAGSGLATQDLAGQGYLAHPLLHDGVPHQGAGRAGQQMLGQVSATSHVNVYLGPEGGLPGPPCVSGQPAGLAATRGYQPCAAYGGGRRQAVPRGSLALQQAQLSDPSPTCRVNGIKVEMQGQPHQLCSNTQSYSGQFYDQTVGFSQQDMKTGSFSISEASCLLQGASAENSELLSPGANQVTSTVDSLDSHDLEGVQIDFDAIIDDGDHASLMSGALSPSIIQNLSHSSSRLTTPRASLPLPTLSVSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	XM_014120825.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris GLI family zinc finger 3 (GLI3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120825.1

ORF Insert Sequence:

ATGCAGCCAC AGAGCGTCCA GGGGCTCGGC AAGATCAGCG AGGAGCCTTC GACGTCTAGC 
GAGGAGAGGG CCTCGCTGAT CAAGAAGGAG ATCCATGGGT CCCTGCCGCA CCTGGCCGAG 
CCCTCGGTGC CGTACCGTGG GGCCGTGTTT GCCATGGACC CCCGGAACGG TTACATGGAG 
CCCCACTACC ACCCTTCTCA TCTCTTTCCT GCATTCCACC CTCCTGTACC AATCGATGCA 
AGACATCATG AGGGGCGTTA CCATTATGAC CCGTCTCCGA TTCCTCCATT GCATGTGCCT 
TCTGCTTTAT CCAGTAGCCC AACGTATTCG GACCTGCCCT TCATCAGGAT CTCCCCACAC 
CGGAACCCCG CTGCAGCCTC TGAACCCCCC TTCAGCCCTC CACACCCCTA CATCAACCCC 
TACATGGACT ACATCCGGTC CCTACACAGC CCGTCCCTCT CCATGATCTC GGCAGCTCGG 
GGGCTCAGCC CTACGGATGC CCCCCATGCT GGGGTCAGCC CAGCGGAATA CTATCATCAG 
ATGGCTCTGC TGGCTGGCCA GCGCAGCCCC TACGCAGACA TCATTCCCTC CGCTGCCACT 
GCCGGCGCTG GGGCCATCCA CATGGAATAT CTTCATGCCA TGGACAGCAC CAGATTCCCC 
AGCCCCAGGC TGTCGGCCCG GCCGAGCCGA AAACGTACAC TGTCCATATC ACCGCTGTCC 
GATCACAGCT TTGACCTTCA GACCATGATA AGGACATCTC CCAACTCCTT GGTCACGATT 
CTCAATAATT CCCGCAGCAG CTCTTCAGCG AGCGGCTCCT ACGGCCACTT GTCTGCGAGT 
GCAATCAGCC CTGCCCTGAG CTTCACCTAC CCTTCTGCAC CCGTGTCTCT CCACATGCAT 
CAGCAAATCT TAAGCCGACA ACAGAGCCTA GGCTCGGCCT TTGGACACAG CCCTCCGCTC 
ATCCACCCTG CTCCAACTTT TCCAACGCAG AGGCCTATTC CTGGGATCCC TACGGTGCTC 
AACCCCGTCC AGGTCACTTC TGGCCCTTCT GAGTCCTCAC AGCAGAACAA GCCGACGAGT 
GAGTCCGCGG TGAGTAGCAC TGGTGACCTG ATGCACAACA AGCGGTCCAA GATCAAACCT 
GACGAAGACC TACCCAGCCC GGGGCCACGG GGCCAGCAGG AACAGCCAGA AGGAACAACC 
CTGGTAAAGG AAGAGGGTGA CAGAGACGAA AGCAAACAAG AGCCCGAAGT CATCTATGAG 
ACCAACTGCC ACTGGGAAGG TTGCACGCGG GAGTTTGACA CCCAGGAGCA GCTGGTGCAC 
CATATAAATA ATGACCATAT TCACGGCGAG AAGAAGGAGT TCGTGTGCCG GTGGATGGAT 
TGCTCGAGGG AGCAGAAGCC CTTCAAAGCC CAGTACATGC TGGTGGTGCA CATGAGGAGA 
CACACCGGGG AGAAACCTCA CAAATGCACT TTTGAAGGTT GTACAAAGGC CTACTCGAGA 
CTAGAAAACT TGAAAACGCA CTTGAGATCT CACACTGGAG AGAAACCGTA CGTCTGTGAG 
CACGAAGGTT GCAACAAGGC TTTCTCAAAT GCCTCTGATC GCGCCAAGCA CCAAAACAGG 
ACGCATTCCA ATGAGAAACC CTATGTGTGC AAAATCCCAG GCTGCACCAA GCGCTACACA 
GACCCAAGTT CCCTCCGGAA ACATGTGAAG ACCGTGCATG GCCCAGAGGC TCATGTCACC 
AAGAAGCAGC GTGGGGACAT ACATCCTCGG CCCCCCCCAC CCCGAGATTC GGGCAGCCAT 
TCACAGTCCA GGTCGCCTGG CCGGCAGACT CAGGGAGCCC TTGGGGAGCA GAAGGACCTC 
AGCAACACTA CCTCAAAGCG GGAAGAATGC CTCCAAGTGA AAGCTGTCAA GGCCGAGAAG 
CCCATGCAGA CATCTCAGCC AAGCCCTGGT GGTGAGTCTT CATGCAGCAG CCAACAGTCC 
CCCATCAGCA ACTATTCCAA CAGTGGGCTC GAGCTTCCTC TGACCGACGG AGGTGGCGTG 
GGAGACCTCG GGACCATCGA CGAAACCCCA ATCATGGACT CGACCATTTC CACCGCAACC 
ACCGCCCTCG CCTTGCAAGC CAGGAGGAAC CCGGCAGGGG CCAAATGGAT GGAGCATGTG 
AAACTAGAAA GGCTCAAACA AGTGAATGGA ATGCTTCCAC GACTGAACCC CGTTCTTCCC 
CCCAAAGCCC CTGCAGTCTC TCCTCTCATA GGAAACGGCA CTCAGCCCAG CACCAGCTGC 
AGTTTGGGCG GGCCCATAAC CCTCCTCCCG AACAGGAGCG ACCTCTCCGG GGTGGACATC 
ACCATGCTGA ACATGCTCAA CCGGAGGGAC AGCAGCGCCA GCACCATCAG CTCCGCCTAC 
CTGAGCAGCC GCCGCTCCTC GGGCATCTCC CCCTGCTTTT CCAGCCGCCG GTCCAGCGAG 
GCGTCCCAGG CCGAGGGCCG GCCCCAGAAC GTGAGCGTGG CCGACTCCTA CGACCCCATC 
TCCACCGACG CGTCGCGCAG GTCCAGCGAG GCGAGCCAGG GCGACGGGCT GCCCAGCCTG 
CTCAGCCTCA CGCCGGCGCA GCAGTACCGC CTGAAGGCCA AGTACGCGGC GGCCACGGGC 
GGGCCGCCCC CCACCCCGCT GCCCAACATG GAGAGGATGA GCCTCAAGAC CAGGATGGCC 
CTGCTCGGGG ACGCCCGGGA GCCCGCGGGG GCCCTGCCGC CCGTGCCCCC TCCGCGGCGG 
TGCAGCGACG GCGGCGCCCA CGGCTACGGC CGCCGCCCCC TGCTGCCCCT GGACGCGCTG 
GGCAACGGCG TGCGCAGGGC CAGCGACCCG GTGCGGACGG CGTCCGACGG CCTCTCCCTG 
CCCCGCGTGC AGCGCTTCGG CAGCCTTCAC AGCTTCAACC CGCCCGGGCC GCCTCCCACC 
CTGGAGAAGC GGCACCTGGT GCTCCAGAAT TACACGCGGC CCGACGGCGG CCAGCCCCGC 
CACTTCCCGC CGTCCCCCTG TCCGCCGAGC ATCACCGAGA ACGTCACCCT GGAGGCCCTG 
ACCATGGACG CCGACGTGAG CCTCACCGAT GAGGACTTCC TGCCCGACGA CGTGGTGCAG 
TATCTGAATT CCCAGAACCC GGCCGGGTTC GAGCAGCCCT TCCAGAGCGC CGTCTCCGAG 
GACAGCAAAG CGCCCCACGG GCCCGGCGAC TTCGACGGCC CGGGGCTGCC GGACGGTCAC 
GCGGGCCACC CAGGCCACCC GGGCCACCCG GGCCCCGCCG GCCAGCCGTA CCGCCCGCTG 
GAGCAGCCGT GCCCCGAAGC CAGCAAAGCC GACCTGCCCA TTCAGTGGAA CGAAGTGAGC 
TCGGGCAGCG CCGACCTGTG CTCCTCCAAG CTCAAGTGTG GCCAGCGGCC GGCGGCGCAG 
CAGGCCCGGG CCTTCGGCTT CTACGGCAAC GTGGGCGCCC CCCCGCAGCC CGCCTTGCGG 
AGCGGGCCCG GCCCCGCCGG CGCCTATGCC CCCCTGGCCG AGCCCGGCCC GGCCTACGGC 
GGCCCCGAGC CCTTGGCGGG CCACGGCGCT GGAGCGGGCA GCCCCGGAGA CGCGTTCCAC 
GAACAGCCCT GCAAGGCCCC GCAGTTCGGG AGCTGCCTGG GCAGGCAGCC GGGCGCCCCC 
GGCCCCCTCG ACGGCGGCTG CGCTGCGGCG GCAAAGCTCA AGAGCAGCAC GGCGCCGGGG 
CCCGGGGGCC AGCCCGACTT TGGCCTGCTG CCGGGGCCCG GCGAGTCAGC GGGCGGCGCC 
GGGAGCGGCC TGGCCACCCA AGACCTGGCA GGACAGGGGT ACCTGGCCCA TCCGCTGCTC 
CACGACGGCG TGCCCCACCA AGGGGCGGGC CGCGCAGGCC AGCAGATGCT AGGGCAGGTT 
AGCGCTACCT CACACGTCAA CGTCTATCTG GGGCCCGAGG GCGGCCTGCC AGGGCCGCCG 
TGCGTCAGCG GCCAGCCCGC CGGCTTGGCG GCCACCCGGG GCTACCAGCC GTGTGCGGCC 
TACGGGGGCG GCAGGCGCCA GGCTGTGCCG AGGGGCAGCC TCGCTCTGCA GCAGGCACAG 
CTCAGTGACC CAAGTCCGAC CTGCAGGGTG AACGGCATCA AGGTGGAGAT GCAGGGGCAG 
CCCCATCAGC TCTGTTCGAA CACGCAGAGT TACTCTGGTC AGTTCTATGA CCAAACCGTT 
GGCTTCAGTC AGCAAGACAT GAAAACTGGT TCATTCTCCA TTTCAGAAGC CAGCTGCCTG 
CTACAGGGGG CCAGCGCCGA GAACTCTGAA TTACTTTCCC CAGGTGCTAA CCAGGTGACG 
AGCACGGTTG ACAGCCTCGA CAGTCATGAC CTGGAAGGGG TGCAGATCGA TTTTGACGCC 
ATCATAGACG ACGGGGACCA CGCCAGCCTG ATGTCAGGAG CCCTGAGTCC CAGTATCATC 
CAGAACCTCT CCCATAGCTC CTCCCGCCTC ACGACACCCC GCGCGTCCCT CCCGCTGCCC 
ACGCTGTCCG TGAGCACCAC CAACATGGCC ATCGGGGACA TGAGCTCCCT GCTGACCTCC 
CTGGCGGAAG AAAGCAAATT CCTTGCAGTG ATGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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GLI3 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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