Gli3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gli3
Entrez Gene ID	140588
Full Name	GLI family zinc finger 3
General protein information	Preferred Names GLI family zinc finger 3 Names transcriptional activator GLI3 GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.1
Summary	transcription factor; member of the C2H2-type zinc finger protein subclass of the Gli family of transcription factors; mediates sonic hedgehog (Shh) signalling; may play a role in embryogenesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_080405.2	NP_536330.2	transcriptional activator GLI3

Pathways from BioSystems

Homologies

Homo sapiens (human)	GLI3	NP_000159.3
Rattus norvegicus (Norway rat)	Gli3	NP_536330.1
Danio rerio (zebrafish)	gli3	NP_991291.1
Pan troglodytes (chimpanzee)	GLI3	NP_001029362.1
Canis lupus familiaris (dog)	GLI3	XP_005630984.1
Bos taurus (cattle)	LOC785371	XP_005205717.1
Gallus gallus (chicken)	GLI3	NP_001258832.1
	GLI3	XP_001098108.1
Mus musculus (house mouse)	Gli3	NP_032156.2
Xenopus tropicalis (tropical clawed frog)	gli3	XP_004915408.1

Gene Ontology
Bibliography

Related articles in PubMed

Maternal exposure to di-n-butyl phthalate (DBP) induces combined anorectal and urogenital malformations in male rat offspring.
Zhu YP, Li EH, Sun WL, Xu DL, Liu ZH, Zhao W, Wood K, Xia SJ, Jiang JT
Reproductive toxicology (Elmsford, N.Y.)61169-76(2016 06)

New insights into genotype-phenotype correlation for GLI3 mutations.
D?murger F, Ichkou A, Mougou-Zerelli S, Le Merrer M, Goudefroye G, Delezoide AL, Qu?lin C, Manouvrier S, Baujat G, Fradin M, Pasquier L, Megarban? A, Faivre L, Baumann C, Nampoothiri S, Roume J, Isidor B, Lacombe D, Delrue MA, Mercier S, Philip N, Schaefer E, Holder M, Krause A, Laffargue F, Sinico M, Amram D, Andr? G, Liquier A, Rossi M, Amiel J, Giuliano F, Boute O, Dieux-Coeslier A, Jacquemont ML, Afenjar A, Van Maldergem L, Lackmy-Port-Lis M, Vincent-Delorme C, Chauvet ML, Cormier-Daire V, Devisme L, Genevi?ve D, Munnich A, Viot G, Raoul O, Romana S, Gonzales M, Encha-Razavi F, Odent S, Vekemans M, Attie-Bitach T
European journal of human genetics : EJHG23(1)92-102(2015 Jan)

A novel GLI3 mutation affecting the zinc finger domain leads to preaxial-postaxial polydactyly-syndactyly complex.
Volodarsky M, Langer Y, Birk OS
BMC medical genetics15110(2014 Sep)

Novel frame-shift mutations of GLI3 gene in non-syndromic postaxial polydactyly patients.
Wang Z, Wang J, Li Y, Geng J, Fu Q, Xu Y, Shen Y
Clinica chimica acta; international journal of clinical chemistry433195-9(2014 Jun)

Primary cilia control hedgehog signaling during muscle differentiation and are deregulated in rhabdomyosarcoma.
Fu W, Asp P, Canter B, Dynlacht BD
Proceedings of the National Academy of Sciences of the United States of America111(25)9151-6(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hoxd13 can directly regulate the expression of Gli3 gene through a Hoxd13 binding site in the limb of rat embryo.
Title: [Mechanism of GLI3 gene transcription regulation in idiopathic congenital talipes equinovarus].

a novel role for Gli3 in regulating calvarial suture development by controlling canonical Bmp-Smad signaling, which integrates a Dlx5/Runx2-II cascade
Title: Prevention of premature fusion of calvarial suture in GLI-Kruppel family member 3 (Gli3)-deficient mice by removing one allele of Runt-related transcription factor 2 (Runx2).

The low expression level of EN1 contribute to the up-regulation of GLI3 in idiopathic congenital talipes equinovarus.
Title: [The functions of GLI3 and EN1 genes in idiopathic congenital talipes equinovarus].

The expression of Gli3, regulated by HOXD13, may play a role in idiopathic congenital talipes equinovarus.
Title: The expression of Gli3, regulated by HOXD13, may play a role in idiopathic congenital talipes equinovarus.

The following Gli3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gli3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa44171	NM_080405.2 Latest version!	Rattus norvegicus GLI family zinc finger 3 (Gli3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gli3 ( NM_080405.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_536330.2 Rattus norvegicus GLI family zinc finger 3 (Gli3), mRNA.

Price & Availability↑

CloneID	ORa44171
Clone ID Related Accession (Same CDS sequence)	NM_080405.2
Accession Version	NM_080405.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Rattus norvegicus(Norway rat)
Product	transcriptional activator GLI3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAHX01091592.1, AAHX01091591.1, AAHX01091588.1, AAHX01091585.1 and AAHX01091584.1. On Sep 25, 2014 this sequence version replaced NM_080405.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the Celera genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCCC AGTCCCACAG CTCGACGACA ACTGAGAGGA AGAAAGCTGA AAGTTCCATT 
GCGAAGTGTC CCACAAGAAC AGATGTCAGT GAGAAGGCCG TGGCCTCCAG TACCACTTCA 
AATGAGGATG AAAGTGCTGG ACAGATCTAT CACCGAGAGA GAAGAAACGC AATCACTATG 
CAGCCTCAGA GTGTGCAGGG TCTCAGCAAA ATCAGTGAGG AGCCCTCAAC ATCTAGTGAT 
GAGAGGGCCT CGTTGATCAA GAAAGAGATC CATGGATCTC TACCACATCT GGCTGAGCCC 
TCTCTCCCTT ATCGTGGGAC TGTGTTTGCC ATGGATCCCC GGAATGGTTA CATGGAGCCT 
CACTACCACC CTCCTCATCT TTTTCCTGCC TTCCATCCTC CTGTACCGAT TGATGCCAGA 
CATCACGAGG GCCGCTACCA TTATGATCCA TCTCCTATTC CTCCATTACA CGTGCCTTCT 
GCCTTATCTA GTAGTCCAAC GTATCCAGAC TTGCCTTTCA TTAGGATCTC CCCACACCGT 
AACCCCACTG CAGCTTCAGA GTCCCCCTTC AGCCCCCCAC ACCCCTACAT CAACCCATAT 
ATGGACTACA TCCGCTCCTT GCACAGCAGC CCATCCCTCT CCATGATCTC CGCTGCCCGA 
GGGCTGAGCC CCACAGATGC CCCCCATGCT GGAGTCAGCC CTGCGGAATA CTATCACCAG 
ATGGCTCTGC TGACAGGCCA GCGCAGCCCC TATGCAGACA TCCTTCCCTC AGCCGCCACT 
GCTGGTGCAG GGGCCATCCA TATGGAGTAC CTTCATGCCA TGGACAGCAC CAGATTTCCC 
AGCCCTAGAC TGTCAGCTAG GCCCAGCCGA AAGCGTACCC TGTCGATCTC TCCACTGTCA 
GATCATAGCT TCGACCTTCA GACCATGATA AGAACATCTC CTAACTCCTT GGTCACAATC 
CTCAATAATT CCCGTAGCAG TTCTTCAGCA AGTGGTTCCT ATGGGCACTT ATCTGCAAGT 
GCAATCAGCC CTGCTTTGAG CTTCACCTAC CCCTCCGCCC CCGTGTCTCT CCATATGCAT 
CAACAGATCC TAAGCCGACA GCAGAGCTTA GGTTCAGCAT TTGGACATAG CCCTCCCCTC 
ATCCACCCTG CTCCAACATT TCCAACGCAG AGACCCATCC CTGGGATTCC GACGGTTCTG 
AACCCTGTCC AGGTCAGCTC TGGCCCTTCT GAGTCTTCAC AGAGCAAACC CACAAGTGAG 
TCTGCAGTGA GTAGTACCGG TGACCCTATG CATAATAAGC GGTCCAAGAT CAAGCCCGAT 
GAAGACCTCC CCAGCCCAGG ATCACGGGGG CAGCAGGAAC AGCCGGAGGG AACAACCCTA 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAGCAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CACAAGAGAG TTCGACACCC AGGATCAGCT TGTGCATCAT 
ATCAATAATG ACCACATTCA TGGAGAAAAG AAGGAGTTTG TGTGCCGCTG GCTTGATTGT 
TCAAGAGAGC AGAAACCGTT CAAAGCCCAG TACATGTTGG TAGTACATAT GAGAAGACAC 
ACCGGAGAGA AGCCTCACAA ATGTACATTT GAAGGTTGTA CAAAAGCCTA CTCAAGACTC 
GAAAACCTGA AAACCCACTT GAGATCTCAC ACTGGAGAGA AGCCATATGT CTGTGAGCAT 
GAGGGCTGCA ACAAGGCTTT CTCTAACGCT TCAGATCGGG CCAAGCACCA AAACAGAACA 
CATTCCAATG AGAAACCATA TGTATGCAAA ATCCCTGGCT GCACCAAGCG TTACACAGAC 
CCCAGCTCTC TCCGGAAACA TGTGAAGACT GTGCATGGCC CTGAGGCTCA TGTTACCAAG 
AAGCAGCGTG GGGACATGCA CCCTCGGCCT CCACCACCTA GAGATTCTGG CAGCCATTCA 
CAGTCCAGGT CCCCCGGCCG GCCGACTCAG GGAGCATTCG GTGAGCAGAA GGAGCTGAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAGA CTGTCAAGGC TGAAAAGCCC 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACTG ATGGAGGTAG TATAGCAGAC 
CTCAGTGCCA TCGATGAGAC CCCAATCATG GACTCGACCA TTTCCACGGC AACCACAGCC 
CTTGCTTTGC AAGGCAGGAG AAACCCAGCA GGGACCAAAT GGATGGAGCA TATCAAACTT 
GAAAGGCTAA AGCAAGTGAA TGGAATGTTT CCAAGACTGA ACCCTATTCT ACCCTCCAAA 
GCCCCTGCGG TATCTCCTCT CATAGGAAAT GGCACACAGT CAAATAACAA CTACAGTTCA 
GGCGGGCCCG GGACCCTTCT CCCGAGCAGA AGTGATCTGT CGGGTGTAGA CTTCACTGTG 
CTGAACACGC TCAACAGGAG GGACAGCAAC ACCAGCACCA TCAGCTCTGC CTACCTGAGC 
AGCCGCAGAT CCTCGGGCAT CTCCCCCTGC TTTTCCAGCC GCAGGTCCAG TGAGGCATCG 
CAGGCTGAAG GGCGACCCCA GAATGTGAGT GTGGCCGACT CCTACGATCC CATCTCCACA 
GATGCTTCAC GGAGGTCCAG CGAGGCCAGC CAGGGTGATG GGCTGCCCAG TCTGCTCAGC 
CTCACACCCG TGCAGCAGTA CCGCCTGAAG GCCAAGTATG CTGCTGCCAC GGGTGGCCCG 
CCACCCACAC CTCTGCCCCA CATGGAAAAG CTGAGCCTGA AGACCAGGAT GGCCCTGCTC 
GGGGAGGGGA GGGACTCAGG GGTGACTCTG CCTCCAGTCC ATCCTCCTCG GAGATGCAGT 
GATGGAGGAG GCCATACATA CAGCAGGCGT CACCTACTGC CTCATGATGC GCTAGCAAAC 
AGTGCGAGGA GAGCCAGCGA CCCTGTGAGG ACCGTCTCCG AGAACATGTC ACTTCCCAGG 
GTTCAACGCT TCAGCAGCCT GAATAGCTTT AATCCTCCAA ATTTGCCTCC ATCCGTGGAA 
AAGCGTAGTT TAGTGCTGCA AAATTATACC CGGCAGGAGA GCACCCATCC CCGGTACTTC 
CAAGCATCCC CTTGCCCTCC CAGCATCACA GAGAATGTTG CCCTGGAGGC CCTGACCATG 
GATGCTGATG CTAGCTTGAA TGATGAAGAT TTCCTGCCAG ATGATGTGGT ACAGTATTTG 
AATTCCCAGA ACCAAACAGG GTATGGGCAG CAATTACAGA GTTCCATCTC TGAAGATGGC 
AAAGTAGCCC GTGAGCCAGA GGACTTGGAC TTACCGGGGC TGCCAGACAG TCATGTTGGC 
CAGCAATACC CAGCTCTGGA GCAACCCTGC TCCGAGGGCA GTAAAACTGA TTTGCCCATC 
CAGTGGAATG AGGTCAGCTC TGGAAGTTCT GATCTGTCAT CCTCCAAGCT GAAGTGTGGT 
CAGCGCCCTA CCGTACAGCA GGCTCGAGGC TTTGGGTTAT ACAGCAATAT GGTGGTACAT 
CCACAGAACC TGTGGAAGGT TGGCACTGGC CCAGCTGGGG GATCTCAGAC CCTTGGGGAG 
AATGGCAGTT CCTACAGTGA CCCAGAGCAC TTTGCAGTCC ACAGTGGGGA TGGACTTGGC 
CCCAGTGGAG ATACTTTCCA TGAACAGGCC TATAAGACCC AGCAGTATGG GAGCCAGCTC 
AGCAGGCAGC CACTGACTTC CAGTGTTCTA GACAGTGCCT GTGGTGCTGG GATTCAAGGG 
TCCAAGCTGA AAGGCAACAG TTTGCAAGAG AATGGGGGTT TGCTAGATTT TGGCCTGTCC 
ATGGCACCAA ATGAGTTAGC TGACAACATA GTGAATGGCA TACAAACCCA AGAACAAATG 
GGGCAGGGGT ACATTGCTCC TCAGCTACTC AGTGGCAGCA TGCAACACCA GGGGCCCAGT 
CGCCCTGGTC AACAGGTACT AGGGCAGGTT GGTGCTACCT CACATATCAA CATCTATCAA 
GGGACAGAGA GCTGCCTACC AGGGACTCAG GACAAGATCA GCCAGCCATC AAGTATGGCA 
GTTATCAGGG GCTACCAGCC CTGTGCCAGC TATGGGGGCA GCAGGCGTCA GGCAATGCCA 
AGGGGCAGCC TCACTCTGCA ACAAGGACAG CTCAGTGACG TGAGTCAGAC CAGCAGGGTG 
AACAGCATCA AAATGGAGGC ACACGGGCAG TCCCATCAGC TCTGCTCTAG CATGCAGAAT 
TATTCCGGTC AGTTTTATGA CCAAACCATG GGCTTCAGTC AGCAAGACAG GAAAGCTGGC 
TCATTCTCCC TCTCAGAGGC CAACTGCCTG CTCCAGGAGA ATGGCTCTGA AAATTCTGAG 
TTACTTTCCC CAGGTGTTAA CCAGGTGACC AGCACAGTTG ACAGCTTTGA GAGTCATGAC 
CTAGAAGGCG TGCAGATTGA TTTTGATGCC ATCATAGATG ATGGGGACCA TACCAGCCTA 
ATGTCAGGGG CCTTGAGCCC CAGTATCATT CAGAACCTTT CCCACAGCTC CTCCCGTCTC 
ACCACACCTC GGGCATCCCT CCCATTCCCT TCTCTGTCCA TGAGCACCAC CAACATGGCT 
ATTGGGGATA TGAGTTCTTT GCTGACCTCC CTTGCAGAAG AAAGCAAGTT CCTTGCAGTT 
ATGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536330.2
CDS	217..4965
Translation	MEAQSHSSTTTERKKAESSIAKCPTRTDVSEKAVASSTTSNEDESAGQIYHRERRNAITMQPQSVQGLSKISEEPSTSSDERASLIKKEIHGSLPHLAEPSLPYRGTVFAMDPRNGYMEPHYHPPHLFPAFHPPVPIDARHHEGRYHYDPSPIPPLHVPSALSSSPTYPDLPFIRISPHRNPTAASESPFSPPHPYINPYMDYIRSLHSSPSLSMISAARGLSPTDAPHAGVSPAEYYHQMALLTGQRSPYADILPSAATAGAGAIHMEYLHAMDSTRFPSPRLSARPSRKRTLSISPLSDHSFDLQTMIRTSPNSLVTILNNSRSSSSASGSYGHLSASAISPALSFTYPSAPVSLHMHQQILSRQQSLGSAFGHSPPLIHPAPTFPTQRPIPGIPTVLNPVQVSSGPSESSQSKPTSESAVSSTGDPMHNKRSKIKPDEDLPSPGSRGQQEQPEGTTLVKEEGDKDESKQEPEVIYETNCHWEGCTREFDTQDQLVHHINNDHIHGEKKEFVCRWLDCSREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGCTKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYVCKIPGCTKRYTDPSSLRKHVKTVHGPEAHVTKKQRGDMHPRPPPPRDSGSHSQSRSPGRPTQGAFGEQKELSNTTSKREECLQVKTVKAEKPMTSQPSPGGQSSCSSQQSPISNYSNSGLELPLTDGGSIADLSAIDETPIMDSTISTATTALALQGRRNPAGTKWMEHIKLERLKQVNGMFPRLNPILPSKAPAVSPLIGNGTQSNNNYSSGGPGTLLPSRSDLSGVDFTVLNTLNRRDSNTSTISSAYLSSRRSSGISPCFSSRRSSEASQAEGRPQNVSVADSYDPISTDASRRSSEASQGDGLPSLLSLTPVQQYRLKAKYAAATGGPPPTPLPHMEKLSLKTRMALLGEGRDSGVTLPPVHPPRRCSDGGGHTYSRRHLLPHDALANSARRASDPVRTVSENMSLPRVQRFSSLNSFNPPNLPPSVEKRSLVLQNYTRQESTHPRYFQASPCPPSITENVALEALTMDADASLNDEDFLPDDVVQYLNSQNQTGYGQQLQSSISEDGKVAREPEDLDLPGLPDSHVGQQYPALEQPCSEGSKTDLPIQWNEVSSGSSDLSSSKLKCGQRPTVQQARGFGLYSNMVVHPQNLWKVGTGPAGGSQTLGENGSSYSDPEHFAVHSGDGLGPSGDTFHEQAYKTQQYGSQLSRQPLTSSVLDSACGAGIQGSKLKGNSLQENGGLLDFGLSMAPNELADNIVNGIQTQEQMGQGYIAPQLLSGSMQHQGPSRPGQQVLGQVGATSHINIYQGTESCLPGTQDKISQPSSMAVIRGYQPCASYGGSRRQAMPRGSLTLQQGQLSDVSQTSRVNSIKMEAHGQSHQLCSSMQNYSGQFYDQTMGFSQQDRKAGSFSLSEANCLLQENGSENSELLSPGVNQVTSTVDSFESHDLEGVQIDFDAIIDDGDHTSLMSGALSPSIIQNLSHSSSRLTTPRASLPFPSLSMSTTNMAIGDMSSLLTSLAEESKFLAVMQ

Target ORF information:

RefSeq Version	NM_080405.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GLI family zinc finger 3 (Gli3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080405.2

ORF Insert Sequence:

ATGGAGGCCC AGTCCCACAG CTCGACGACA ACTGAGAGGA AGAAAGCTGA AAGTTCCATT 
GCGAAGTGTC CCACAAGAAC AGATGTCAGT GAGAAGGCCG TGGCCTCCAG TACCACTTCA 
AATGAGGATG AAAGTGCTGG ACAGATCTAT CACCGAGAGA GAAGAAACGC AATCACTATG 
CAGCCTCAGA GTGTGCAGGG TCTCAGCAAA ATCAGTGAGG AGCCCTCAAC ATCTAGTGAT 
GAGAGGGCCT CGTTGATCAA GAAAGAGATC CATGGATCTC TACCACATCT GGCTGAGCCC 
TCTCTCCCTT ATCGTGGGAC TGTGTTTGCC ATGGATCCCC GGAATGGTTA CATGGAGCCT 
CACTACCACC CTCCTCATCT TTTTCCTGCC TTCCATCCTC CTGTACCGAT TGATGCCAGA 
CATCACGAGG GCCGCTACCA TTATGATCCA TCTCCTATTC CTCCATTACA CGTGCCTTCT 
GCCTTATCTA GTAGTCCAAC GTATCCAGAC TTGCCTTTCA TTAGGATCTC CCCACACCGT 
AACCCCACTG CAGCTTCAGA GTCCCCCTTC AGCCCCCCAC ACCCCTACAT CAACCCATAT 
ATGGACTACA TCCGCTCCTT GCACAGCAGC CCATCCCTCT CCATGATCTC CGCTGCCCGA 
GGGCTGAGCC CCACAGATGC CCCCCATGCT GGAGTCAGCC CTGCGGAATA CTATCACCAG 
ATGGCTCTGC TGACAGGCCA GCGCAGCCCC TATGCAGACA TCCTTCCCTC AGCCGCCACT 
GCTGGTGCAG GGGCCATCCA TATGGAGTAC CTTCATGCCA TGGACAGCAC CAGATTTCCC 
AGCCCTAGAC TGTCAGCTAG GCCCAGCCGA AAGCGTACCC TGTCGATCTC TCCACTGTCA 
GATCATAGCT TCGACCTTCA GACCATGATA AGAACATCTC CTAACTCCTT GGTCACAATC 
CTCAATAATT CCCGTAGCAG TTCTTCAGCA AGTGGTTCCT ATGGGCACTT ATCTGCAAGT 
GCAATCAGCC CTGCTTTGAG CTTCACCTAC CCCTCCGCCC CCGTGTCTCT CCATATGCAT 
CAACAGATCC TAAGCCGACA GCAGAGCTTA GGTTCAGCAT TTGGACATAG CCCTCCCCTC 
ATCCACCCTG CTCCAACATT TCCAACGCAG AGACCCATCC CTGGGATTCC GACGGTTCTG 
AACCCTGTCC AGGTCAGCTC TGGCCCTTCT GAGTCTTCAC AGAGCAAACC CACAAGTGAG 
TCTGCAGTGA GTAGTACCGG TGACCCTATG CATAATAAGC GGTCCAAGAT CAAGCCCGAT 
GAAGACCTCC CCAGCCCAGG ATCACGGGGG CAGCAGGAAC AGCCGGAGGG AACAACCCTA 
GTCAAGGAGG AAGGGGACAA AGATGAAAGC AAGCAGGAGC CTGAAGTCAT CTATGAGACA 
AACTGCCACT GGGAAGGCTG CACAAGAGAG TTCGACACCC AGGATCAGCT TGTGCATCAT 
ATCAATAATG ACCACATTCA TGGAGAAAAG AAGGAGTTTG TGTGCCGCTG GCTTGATTGT 
TCAAGAGAGC AGAAACCGTT CAAAGCCCAG TACATGTTGG TAGTACATAT GAGAAGACAC 
ACCGGAGAGA AGCCTCACAA ATGTACATTT GAAGGTTGTA CAAAAGCCTA CTCAAGACTC 
GAAAACCTGA AAACCCACTT GAGATCTCAC ACTGGAGAGA AGCCATATGT CTGTGAGCAT 
GAGGGCTGCA ACAAGGCTTT CTCTAACGCT TCAGATCGGG CCAAGCACCA AAACAGAACA 
CATTCCAATG AGAAACCATA TGTATGCAAA ATCCCTGGCT GCACCAAGCG TTACACAGAC 
CCCAGCTCTC TCCGGAAACA TGTGAAGACT GTGCATGGCC CTGAGGCTCA TGTTACCAAG 
AAGCAGCGTG GGGACATGCA CCCTCGGCCT CCACCACCTA GAGATTCTGG CAGCCATTCA 
CAGTCCAGGT CCCCCGGCCG GCCGACTCAG GGAGCATTCG GTGAGCAGAA GGAGCTGAGC 
AACACTACCT CAAAGCGGGA AGAATGCCTC CAGGTGAAGA CTGTCAAGGC TGAAAAGCCC 
ATGACATCTC AGCCAAGCCC TGGTGGTCAG TCTTCATGCA GCAGCCAACA GTCCCCCATC 
AGCAACTATT CCAACAGTGG GCTCGAGCTT CCTCTGACTG ATGGAGGTAG TATAGCAGAC 
CTCAGTGCCA TCGATGAGAC CCCAATCATG GACTCGACCA TTTCCACGGC AACCACAGCC 
CTTGCTTTGC AAGGCAGGAG AAACCCAGCA GGGACCAAAT GGATGGAGCA TATCAAACTT 
GAAAGGCTAA AGCAAGTGAA TGGAATGTTT CCAAGACTGA ACCCTATTCT ACCCTCCAAA 
GCCCCTGCGG TATCTCCTCT CATAGGAAAT GGCACACAGT CAAATAACAA CTACAGTTCA 
GGCGGGCCCG GGACCCTTCT CCCGAGCAGA AGTGATCTGT CGGGTGTAGA CTTCACTGTG 
CTGAACACGC TCAACAGGAG GGACAGCAAC ACCAGCACCA TCAGCTCTGC CTACCTGAGC 
AGCCGCAGAT CCTCGGGCAT CTCCCCCTGC TTTTCCAGCC GCAGGTCCAG TGAGGCATCG 
CAGGCTGAAG GGCGACCCCA GAATGTGAGT GTGGCCGACT CCTACGATCC CATCTCCACA 
GATGCTTCAC GGAGGTCCAG CGAGGCCAGC CAGGGTGATG GGCTGCCCAG TCTGCTCAGC 
CTCACACCCG TGCAGCAGTA CCGCCTGAAG GCCAAGTATG CTGCTGCCAC GGGTGGCCCG 
CCACCCACAC CTCTGCCCCA CATGGAAAAG CTGAGCCTGA AGACCAGGAT GGCCCTGCTC 
GGGGAGGGGA GGGACTCAGG GGTGACTCTG CCTCCAGTCC ATCCTCCTCG GAGATGCAGT 
GATGGAGGAG GCCATACATA CAGCAGGCGT CACCTACTGC CTCATGATGC GCTAGCAAAC 
AGTGCGAGGA GAGCCAGCGA CCCTGTGAGG ACCGTCTCCG AGAACATGTC ACTTCCCAGG 
GTTCAACGCT TCAGCAGCCT GAATAGCTTT AATCCTCCAA ATTTGCCTCC ATCCGTGGAA 
AAGCGTAGTT TAGTGCTGCA AAATTATACC CGGCAGGAGA GCACCCATCC CCGGTACTTC 
CAAGCATCCC CTTGCCCTCC CAGCATCACA GAGAATGTTG CCCTGGAGGC CCTGACCATG 
GATGCTGATG CTAGCTTGAA TGATGAAGAT TTCCTGCCAG ATGATGTGGT ACAGTATTTG 
AATTCCCAGA ACCAAACAGG GTATGGGCAG CAATTACAGA GTTCCATCTC TGAAGATGGC 
AAAGTAGCCC GTGAGCCAGA GGACTTGGAC TTACCGGGGC TGCCAGACAG TCATGTTGGC 
CAGCAATACC CAGCTCTGGA GCAACCCTGC TCCGAGGGCA GTAAAACTGA TTTGCCCATC 
CAGTGGAATG AGGTCAGCTC TGGAAGTTCT GATCTGTCAT CCTCCAAGCT GAAGTGTGGT 
CAGCGCCCTA CCGTACAGCA GGCTCGAGGC TTTGGGTTAT ACAGCAATAT GGTGGTACAT 
CCACAGAACC TGTGGAAGGT TGGCACTGGC CCAGCTGGGG GATCTCAGAC CCTTGGGGAG 
AATGGCAGTT CCTACAGTGA CCCAGAGCAC TTTGCAGTCC ACAGTGGGGA TGGACTTGGC 
CCCAGTGGAG ATACTTTCCA TGAACAGGCC TATAAGACCC AGCAGTATGG GAGCCAGCTC 
AGCAGGCAGC CACTGACTTC CAGTGTTCTA GACAGTGCCT GTGGTGCTGG GATTCAAGGG 
TCCAAGCTGA AAGGCAACAG TTTGCAAGAG AATGGGGGTT TGCTAGATTT TGGCCTGTCC 
ATGGCACCAA ATGAGTTAGC TGACAACATA GTGAATGGCA TACAAACCCA AGAACAAATG 
GGGCAGGGGT ACATTGCTCC TCAGCTACTC AGTGGCAGCA TGCAACACCA GGGGCCCAGT 
CGCCCTGGTC AACAGGTACT AGGGCAGGTT GGTGCTACCT CACATATCAA CATCTATCAA 
GGGACAGAGA GCTGCCTACC AGGGACTCAG GACAAGATCA GCCAGCCATC AAGTATGGCA 
GTTATCAGGG GCTACCAGCC CTGTGCCAGC TATGGGGGCA GCAGGCGTCA GGCAATGCCA 
AGGGGCAGCC TCACTCTGCA ACAAGGACAG CTCAGTGACG TGAGTCAGAC CAGCAGGGTG 
AACAGCATCA AAATGGAGGC ACACGGGCAG TCCCATCAGC TCTGCTCTAG CATGCAGAAT 
TATTCCGGTC AGTTTTATGA CCAAACCATG GGCTTCAGTC AGCAAGACAG GAAAGCTGGC 
TCATTCTCCC TCTCAGAGGC CAACTGCCTG CTCCAGGAGA ATGGCTCTGA AAATTCTGAG 
TTACTTTCCC CAGGTGTTAA CCAGGTGACC AGCACAGTTG ACAGCTTTGA GAGTCATGAC 
CTAGAAGGCG TGCAGATTGA TTTTGATGCC ATCATAGATG ATGGGGACCA TACCAGCCTA 
ATGTCAGGGG CCTTGAGCCC CAGTATCATT CAGAACCTTT CCCACAGCTC CTCCCGTCTC 
ACCACACCTC GGGCATCCCT CCCATTCCCT TCTCTGTCCA TGAGCACCAC CAACATGGCT 
ATTGGGGATA TGAGTTCTTT GCTGACCTCC CTTGCAGAAG AAAGCAAGTT CCTTGCAGTT 
ATGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Maternal exposure to di-n-butyl phthalate (DBP) induces combined anorectal and urogenital malformations in male rat offspring.
Reproductive toxicology (Elmsford, N.Y.)61169-76(2016 06)
Zhu YP,Li EH,Sun WL,Xu DL,Liu ZH,Zhao W,Wood K,Xia SJ,Jiang JT

New insights into genotype-phenotype correlation for GLI3 mutations.
European journal of human genetics : EJHG23(1)92-102(2015 Jan)
D?murger F,Ichkou A,Mougou-Zerelli S,Le Merrer M,Goudefroye G,Delezoide AL,Qu?lin C,Manouvrier S,Baujat G,Fradin M,Pasquier L,Megarban? A,Faivre L,Baumann C,Nampoothiri S,Roume J,Isidor B,Lacombe D,Delrue MA,Mercier S,Philip N,Schaefer E,Holder M,Krause A,Laffargue F,Sinico M,Amram D,Andr? G,Liquier A,Rossi M,Amiel J,Giuliano F,Boute O,Dieux-Coeslier A,Jacquemont ML,Afenjar A,Van Maldergem L,Lackmy-Port-Lis M,Vincent-Delorme C,Chauvet ML,Cormier-Daire V,Devisme L,Genevi?ve D,Munnich A,Viot G,Raoul O,Romana S,Gonzales M,Encha-Razavi F,Odent S,Vekemans M,Attie-Bitach T

A novel GLI3 mutation affecting the zinc finger domain leads to preaxial-postaxial polydactyly-syndactyly complex.
BMC medical genetics15110(2014 Sep)
Volodarsky M,Langer Y,Birk OS

Novel frame-shift mutations of GLI3 gene in non-syndromic postaxial polydactyly patients.
Clinica chimica acta; international journal of clinical chemistry433195-9(2014 Jun)
Wang Z,Wang J,Li Y,Geng J,Fu Q,Xu Y,Shen Y

Primary cilia control hedgehog signaling during muscle differentiation and are deregulated in rhabdomyosarcoma.
Proceedings of the National Academy of Sciences of the United States of America111(25)9151-6(2014 Jun)
Fu W,Asp P,Canter B,Dynlacht BD

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Gli3 cDNA ORF clone, Rattus norvegicus(Norway rat)

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ORF Insert Sequence:

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