gRNAs
The following Gli3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gli3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu78425 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017315389.1 | |
| Accession Version | XM_017315389.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | transcriptional activator GLI3 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTCTTGTT CTCACTTCCT AAGGACAGTG GGTAATAAGA CATCTCAGCC AAGCCCTGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017170878.1 |
| CDS | 49..2736 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_017315389.1 |
1 | ATGTCTTGTT CTCACTTCCT AAGGACAGTG GGTAATAAGA CATCTCAGCC AAGCCCTGGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu21819 | |
| Clone ID Related Accession (Same CDS sequence) | NM_008130.2 , NM_008130.3 | |
| Accession Version | NM_008130.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-12-30 | |
| Organism | Mus musculus(house mouse) | |
| Product | transcriptional activator GLI3 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK158261.1, X95255.1, CA752350.1 and AC173210.3. On Jan 5, 2007 this sequence version replaced NM_008130.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X95255.1, BC141135.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_032156.2 |
| CDS | 432..5183 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_008130.2 |
1 | ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu21819 | |
| Clone ID Related Accession (Same CDS sequence) | NM_008130.2 , NM_008130.3 | |
| Accession Version | NM_008130.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-29 | |
| Organism | Mus musculus(house mouse) | |
| Product | transcriptional activator GLI3 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC163610.4, AC173115.2 and AC173210.3. On Aug 14, 2018 this sequence version replaced NM_008130.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC141135.1, X95255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_032156.2 |
| CDS | 431..5182 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_008130.3 |
1 | ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu48321 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006516552.3 | |
| Accession Version | XM_006516552.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | transcriptional activator GLI3 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006516552.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006516615.1 |
| CDS | 130..4884 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006516552.3 |
1 | ATGGAGGCCC AGGCCCACAG CTCTACGGCG ACTGAGAGGA AGAAAGCTGA AAATTCCATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
