ORF » Species Summary » Homo sapiens » GCLC cDNA ORF clone

GCLC cDNA ORF clone, Homo sapiens(human)

The following GCLC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCLC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu18571 NM_001498.4
Latest version!		 Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$189.50-$265.30
$379.00
OHu55651 NM_001197115.2
Latest version!		 Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $349.30
$499.00
OHu96543 XM_017010749.1
Latest version!		 Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
View Old Accession Versions >>
OHu18571 NM_001498.3 Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$189.50-$265.30
$379.00
OHu55651 NM_001197115.1 Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $349.30
$499.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    GCLC ( NM_001498.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001489.1 Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu18571
    Clone ID Related Accession (Same CDS sequence) NM_001498.3 , NM_001498.4
    Accession Version NM_001498.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-17
    Organism Homo sapiens(human)
    Product glutamate--cysteine ligase catalytic subunit isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI550814.1, AL531593.3, AK313480.1, AB262176.1 and BC039894.1. This sequence is a reference standard in the RefSeqGene project. On Oct 7, 2010 this sequence version replaced NM_001498.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC039894.1, AB262176.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AAACCAAGCG CCATGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
    CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
    GAAAATAAAA AAGTCCGGTT GGTCCTGTCT GGGGAGAAAG TTCTTGAAAC TCTGCAAGAG 
    AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTTTGGA GACCAGAGTA TGGGAGTTAC 
    ATGATTGAAG GGACACCAGG ACAGCCCTAC GGAGGAACAA TGTCCGAGTT CAATACAGTT 
    GAGGCCAACA TGCGAAAACG CCGGAAGGAG GCTACTTCTA TATTAGAAGA AAATCAGGCT 
    CTTTGCACAA TAACTTCATT TCCCAGATTA GGCTGTCCTG GGTTCACACT GCCCGAGGTC 
    AAACCCAACC CAGTGGAAGG AGGAGCTTCC AAGTCCCTCT TCTTTCCAGA TGAAGCAATA 
    AACAAGCACC CTCGCTTCAG TACCTTAACA AGAAATATCC GACATAGGAG AGGAGAAAAG 
    GTTGTCATCA ATGTACCAAT ATTTAAGGAC AAGAATACAC CATCTCCATT TATAGAAACA 
    TTTACTGAGG ATGATGAAGC TTCAAGGGCT TCTAAGCCGG ATCATATTTA CATGGATGCC 
    ATGGGATTTG GAATGGGCAA TTGCTGTCTC CAGGTGACAT TCCAAGCCTG CAGTATATCT 
    GAGGCCAGAT ACCTTTATGA TCAGTTGGCT ACTATCTGTC CAATTGTTAT GGCTTTGAGT 
    GCTGCATCTC CCTTTTACCG AGGCTATGTG TCAGACATTG ATTGTCGCTG GGGAGTGATT 
    TCTGCATCTG TAGATGATAG AACTCGGGAG GAGCGAGGAC TGGAGCCATT GAAGAACAAT 
    AACTATAGGA TCAGTAAATC CCGATATGAC TCAATAGACA GCTATTTATC TAAGTGTGGT 
    GAGAAATATA ATGACATCGA CTTGACGATA GATAAAGAGA TCTACGAACA GCTGTTGCAG 
    GAAGGCATTG ATCATCTCCT GGCCCAGCAT GTTGCTCATC TCTTTATTAG AGACCCACTG 
    ACACTGTTTG AAGAGAAAAT ACACCTGGAT GATGCTAATG AGTCTGACCA TTTTGAGAAT 
    ATTCAGTCCA CAAATTGGCA GACAATGAGA TTTAAGCCCC CTCCTCCAAA CTCAGACATT 
    GGATGGAGAG TAGAATTTCG ACCCATGGAG GTGCAATTAA CAGACTTTGA GAACTCTGCC 
    TATGTGGTGT TTGTGGTACT GCTCACCAGA GTGATCCTTT CCTACAAATT GGATTTTCTC 
    ATTCCACTGT CAAAGGTTGA TGAGAACATG AAGGTAGCAC AGAAAAGAGA TGCTGTCTTG 
    CAGGGAATGT TTTATTTCAG GAAAGATATT TGCAAAGGTG GCAATGCAGT GGTGGATGGT 
    TGTGGCAAGG CCCAGAACAG CACGGAGCTC GCTGCAGAGG AGTACACCCT CATGAGCATA 
    GACACCATCA TCAATGGGAA GGAAGGTGTG TTTCCTGGAC TGATCCCAAT TCTGAACTCT 
    TACCTTGAAA ACATGGAAGT GGATGTGGAC ACCAGATGTA GTATTCTGAA CTACCTAAAG 
    CTAATTAAGA AGAGAGCATC TGGAGAACTA ATGACAGTTG CCAGATGGAT GAGGGAGTTT 
    ATCGCAAACC ATCCTGACTA CAAGCAAGAC AGTGTCATAA CTGATGAAAT GAATTATAGC 
    CTTATTTTGA AGTGTAACCA AATTGCAAAT GAATTATGTG AATGCCCAGA GTTACTTGGA 
    TCAGCATTTA GGAAAGTAAA ATATAGTGGA AGTAAAACTG ACTCATCCAA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001489.1
    CDS485..2398
    Translation

    Target ORF information:

    RefSeq Version NM_001498.3
    Organism Homo sapiens(human)
    Definition Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001498.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AAACCAAGCG CCATGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
    CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
    GAAAATAAAA AAGTCCGGTT GGTCCTGTCT GGGGAGAAAG TTCTTGAAAC TCTGCAAGAG 
    AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTTTGGA GACCAGAGTA TGGGAGTTAC 
    ATGATTGAAG GGACACCAGG ACAGCCCTAC GGAGGAACAA TGTCCGAGTT CAATACAGTT 
    GAGGCCAACA TGCGAAAACG CCGGAAGGAG GCTACTTCTA TATTAGAAGA AAATCAGGCT 
    CTTTGCACAA TAACTTCATT TCCCAGATTA GGCTGTCCTG GGTTCACACT GCCCGAGGTC 
    AAACCCAACC CAGTGGAAGG AGGAGCTTCC AAGTCCCTCT TCTTTCCAGA TGAAGCAATA 
    AACAAGCACC CTCGCTTCAG TACCTTAACA AGAAATATCC GACATAGGAG AGGAGAAAAG 
    GTTGTCATCA ATGTACCAAT ATTTAAGGAC AAGAATACAC CATCTCCATT TATAGAAACA 
    TTTACTGAGG ATGATGAAGC TTCAAGGGCT TCTAAGCCGG ATCATATTTA CATGGATGCC 
    ATGGGATTTG GAATGGGCAA TTGCTGTCTC CAGGTGACAT TCCAAGCCTG CAGTATATCT 
    GAGGCCAGAT ACCTTTATGA TCAGTTGGCT ACTATCTGTC CAATTGTTAT GGCTTTGAGT 
    GCTGCATCTC CCTTTTACCG AGGCTATGTG TCAGACATTG ATTGTCGCTG GGGAGTGATT 
    TCTGCATCTG TAGATGATAG AACTCGGGAG GAGCGAGGAC TGGAGCCATT GAAGAACAAT 
    AACTATAGGA TCAGTAAATC CCGATATGAC TCAATAGACA GCTATTTATC TAAGTGTGGT 
    GAGAAATATA ATGACATCGA CTTGACGATA GATAAAGAGA TCTACGAACA GCTGTTGCAG 
    GAAGGCATTG ATCATCTCCT GGCCCAGCAT GTTGCTCATC TCTTTATTAG AGACCCACTG 
    ACACTGTTTG AAGAGAAAAT ACACCTGGAT GATGCTAATG AGTCTGACCA TTTTGAGAAT 
    ATTCAGTCCA CAAATTGGCA GACAATGAGA TTTAAGCCCC CTCCTCCAAA CTCAGACATT 
    GGATGGAGAG TAGAATTTCG ACCCATGGAG GTGCAATTAA CAGACTTTGA GAACTCTGCC 
    TATGTGGTGT TTGTGGTACT GCTCACCAGA GTGATCCTTT CCTACAAATT GGATTTTCTC 
    ATTCCACTGT CAAAGGTTGA TGAGAACATG AAGGTAGCAC AGAAAAGAGA TGCTGTCTTG 
    CAGGGAATGT TTTATTTCAG GAAAGATATT TGCAAAGGTG GCAATGCAGT GGTGGATGGT 
    TGTGGCAAGG CCCAGAACAG CACGGAGCTC GCTGCAGAGG AGTACACCCT CATGAGCATA 
    GACACCATCA TCAATGGGAA GGAAGGTGTG TTTCCTGGAC TGATCCCAAT TCTGAACTCT 
    TACCTTGAAA ACATGGAAGT GGATGTGGAC ACCAGATGTA GTATTCTGAA CTACCTAAAG 
    CTAATTAAGA AGAGAGCATC TGGAGAACTA ATGACAGTTG CCAGATGGAT GAGGGAGTTT 
    ATCGCAAACC ATCCTGACTA CAAGCAAGAC AGTGTCATAA CTGATGAAAT GAATTATAGC 
    CTTATTTTGA AGTGTAACCA AATTGCAAAT GAATTATGTG AATGCCCAGA GTTACTTGGA 
    TCAGCATTTA GGAAAGTAAA ATATAGTGGA AGTAAAACTG ACTCATCCAA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    GCLC ( NM_001498.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001489.1 Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu18571
    Clone ID Related Accession (Same CDS sequence) NM_001498.3 , NM_001498.4
    Accession Version NM_001498.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-05
    Organism Homo sapiens(human)
    Product glutamate--cysteine ligase catalytic subunit isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI550814.1, AL531593.3, AK313480.1, AB262176.1 and BC039894.1. This sequence is a reference standard in the RefSeqGene project. On May 8, 2018 this sequence version replaced NM_001498.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.95345.1, AB262176.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000650454.1/ ENSP00000497574.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AAACCAAGCG CCATGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
    CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
    GAAAATAAAA AAGTCCGGTT GGTCCTGTCT GGGGAGAAAG TTCTTGAAAC TCTGCAAGAG 
    AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTTTGGA GACCAGAGTA TGGGAGTTAC 
    ATGATTGAAG GGACACCAGG ACAGCCCTAC GGAGGAACAA TGTCCGAGTT CAATACAGTT 
    GAGGCCAACA TGCGAAAACG CCGGAAGGAG GCTACTTCTA TATTAGAAGA AAATCAGGCT 
    CTTTGCACAA TAACTTCATT TCCCAGATTA GGCTGTCCTG GGTTCACACT GCCCGAGGTC 
    AAACCCAACC CAGTGGAAGG AGGAGCTTCC AAGTCCCTCT TCTTTCCAGA TGAAGCAATA 
    AACAAGCACC CTCGCTTCAG TACCTTAACA AGAAATATCC GACATAGGAG AGGAGAAAAG 
    GTTGTCATCA ATGTACCAAT ATTTAAGGAC AAGAATACAC CATCTCCATT TATAGAAACA 
    TTTACTGAGG ATGATGAAGC TTCAAGGGCT TCTAAGCCGG ATCATATTTA CATGGATGCC 
    ATGGGATTTG GAATGGGCAA TTGCTGTCTC CAGGTGACAT TCCAAGCCTG CAGTATATCT 
    GAGGCCAGAT ACCTTTATGA TCAGTTGGCT ACTATCTGTC CAATTGTTAT GGCTTTGAGT 
    GCTGCATCTC CCTTTTACCG AGGCTATGTG TCAGACATTG ATTGTCGCTG GGGAGTGATT 
    TCTGCATCTG TAGATGATAG AACTCGGGAG GAGCGAGGAC TGGAGCCATT GAAGAACAAT 
    AACTATAGGA TCAGTAAATC CCGATATGAC TCAATAGACA GCTATTTATC TAAGTGTGGT 
    GAGAAATATA ATGACATCGA CTTGACGATA GATAAAGAGA TCTACGAACA GCTGTTGCAG 
    GAAGGCATTG ATCATCTCCT GGCCCAGCAT GTTGCTCATC TCTTTATTAG AGACCCACTG 
    ACACTGTTTG AAGAGAAAAT ACACCTGGAT GATGCTAATG AGTCTGACCA TTTTGAGAAT 
    ATTCAGTCCA CAAATTGGCA GACAATGAGA TTTAAGCCCC CTCCTCCAAA CTCAGACATT 
    GGATGGAGAG TAGAATTTCG ACCCATGGAG GTGCAATTAA CAGACTTTGA GAACTCTGCC 
    TATGTGGTGT TTGTGGTACT GCTCACCAGA GTGATCCTTT CCTACAAATT GGATTTTCTC 
    ATTCCACTGT CAAAGGTTGA TGAGAACATG AAGGTAGCAC AGAAAAGAGA TGCTGTCTTG 
    CAGGGAATGT TTTATTTCAG GAAAGATATT TGCAAAGGTG GCAATGCAGT GGTGGATGGT 
    TGTGGCAAGG CCCAGAACAG CACGGAGCTC GCTGCAGAGG AGTACACCCT CATGAGCATA 
    GACACCATCA TCAATGGGAA GGAAGGTGTG TTTCCTGGAC TGATCCCAAT TCTGAACTCT 
    TACCTTGAAA ACATGGAAGT GGATGTGGAC ACCAGATGTA GTATTCTGAA CTACCTAAAG 
    CTAATTAAGA AGAGAGCATC TGGAGAACTA ATGACAGTTG CCAGATGGAT GAGGGAGTTT 
    ATCGCAAACC ATCCTGACTA CAAGCAAGAC AGTGTCATAA CTGATGAAAT GAATTATAGC 
    CTTATTTTGA AGTGTAACCA AATTGCAAAT GAATTATGTG AATGCCCAGA GTTACTTGGA 
    TCAGCATTTA GGAAAGTAAA ATATAGTGGA AGTAAAACTG ACTCATCCAA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001489.1
    CDS457..2370
    Translation

    Target ORF information:

    RefSeq Version NM_001498.4
    Organism Homo sapiens(human)
    Definition Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001498.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AAACCAAGCG CCATGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
    CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
    GAAAATAAAA AAGTCCGGTT GGTCCTGTCT GGGGAGAAAG TTCTTGAAAC TCTGCAAGAG 
    AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTTTGGA GACCAGAGTA TGGGAGTTAC 
    ATGATTGAAG GGACACCAGG ACAGCCCTAC GGAGGAACAA TGTCCGAGTT CAATACAGTT 
    GAGGCCAACA TGCGAAAACG CCGGAAGGAG GCTACTTCTA TATTAGAAGA AAATCAGGCT 
    CTTTGCACAA TAACTTCATT TCCCAGATTA GGCTGTCCTG GGTTCACACT GCCCGAGGTC 
    AAACCCAACC CAGTGGAAGG AGGAGCTTCC AAGTCCCTCT TCTTTCCAGA TGAAGCAATA 
    AACAAGCACC CTCGCTTCAG TACCTTAACA AGAAATATCC GACATAGGAG AGGAGAAAAG 
    GTTGTCATCA ATGTACCAAT ATTTAAGGAC AAGAATACAC CATCTCCATT TATAGAAACA 
    TTTACTGAGG ATGATGAAGC TTCAAGGGCT TCTAAGCCGG ATCATATTTA CATGGATGCC 
    ATGGGATTTG GAATGGGCAA TTGCTGTCTC CAGGTGACAT TCCAAGCCTG CAGTATATCT 
    GAGGCCAGAT ACCTTTATGA TCAGTTGGCT ACTATCTGTC CAATTGTTAT GGCTTTGAGT 
    GCTGCATCTC CCTTTTACCG AGGCTATGTG TCAGACATTG ATTGTCGCTG GGGAGTGATT 
    TCTGCATCTG TAGATGATAG AACTCGGGAG GAGCGAGGAC TGGAGCCATT GAAGAACAAT 
    AACTATAGGA TCAGTAAATC CCGATATGAC TCAATAGACA GCTATTTATC TAAGTGTGGT 
    GAGAAATATA ATGACATCGA CTTGACGATA GATAAAGAGA TCTACGAACA GCTGTTGCAG 
    GAAGGCATTG ATCATCTCCT GGCCCAGCAT GTTGCTCATC TCTTTATTAG AGACCCACTG 
    ACACTGTTTG AAGAGAAAAT ACACCTGGAT GATGCTAATG AGTCTGACCA TTTTGAGAAT 
    ATTCAGTCCA CAAATTGGCA GACAATGAGA TTTAAGCCCC CTCCTCCAAA CTCAGACATT 
    GGATGGAGAG TAGAATTTCG ACCCATGGAG GTGCAATTAA CAGACTTTGA GAACTCTGCC 
    TATGTGGTGT TTGTGGTACT GCTCACCAGA GTGATCCTTT CCTACAAATT GGATTTTCTC 
    ATTCCACTGT CAAAGGTTGA TGAGAACATG AAGGTAGCAC AGAAAAGAGA TGCTGTCTTG 
    CAGGGAATGT TTTATTTCAG GAAAGATATT TGCAAAGGTG GCAATGCAGT GGTGGATGGT 
    TGTGGCAAGG CCCAGAACAG CACGGAGCTC GCTGCAGAGG AGTACACCCT CATGAGCATA 
    GACACCATCA TCAATGGGAA GGAAGGTGTG TTTCCTGGAC TGATCCCAAT TCTGAACTCT 
    TACCTTGAAA ACATGGAAGT GGATGTGGAC ACCAGATGTA GTATTCTGAA CTACCTAAAG 
    CTAATTAAGA AGAGAGCATC TGGAGAACTA ATGACAGTTG CCAGATGGAT GAGGGAGTTT 
    ATCGCAAACC ATCCTGACTA CAAGCAAGAC AGTGTCATAA CTGATGAAAT GAATTATAGC 
    CTTATTTTGA AGTGTAACCA AATTGCAAAT GAATTATGTG AATGCCCAGA GTTACTTGGA 
    TCAGCATTTA GGAAAGTAAA ATATAGTGGA AGTAAAACTG ACTCATCCAA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    GCLC ( NM_001197115.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001184044.1 Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu55651
    Clone ID Related Accession (Same CDS sequence) NM_001197115.1 , NM_001197115.2
    Accession Version NM_001197115.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product glutamate--cysteine ligase catalytic subunit isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI550814.1, AL531593.3, AK313480.1, AB516284.1, AB262176.1 and BC039894.1. Transcript Variant: This variant (2, also referred to as delta4) lacks an in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform b), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB516284.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AAACCAAGCG CCATGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
    CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
    GAAAATAAAA AAGTCCGGTT GGTCCTGTCT GGGGAGAAAG TTCTTGAAAC TCTGCAAGAG 
    AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTTTGGA GACCAGAGTA TGGGAGTTAC 
    ATGATTGAAG GGACACCAGG ACAGCCCTAC GGAGGAACAA TGTCCGAGTT CAATACAGTT 
    GAGGCCAACA TGCGAAAACG CCGGAAGGAG GCTACTTCTA TATTAGAAGA AAATCAGGCT 
    CTTTGCACAA TAACTTCATT TCCCAGTACC TTAACAAGAA ATATCCGACA TAGGAGAGGA 
    GAAAAGGTTG TCATCAATGT ACCAATATTT AAGGACAAGA ATACACCATC TCCATTTATA 
    GAAACATTTA CTGAGGATGA TGAAGCTTCA AGGGCTTCTA AGCCGGATCA TATTTACATG 
    GATGCCATGG GATTTGGAAT GGGCAATTGC TGTCTCCAGG TGACATTCCA AGCCTGCAGT 
    ATATCTGAGG CCAGATACCT TTATGATCAG TTGGCTACTA TCTGTCCAAT TGTTATGGCT 
    TTGAGTGCTG CATCTCCCTT TTACCGAGGC TATGTGTCAG ACATTGATTG TCGCTGGGGA 
    GTGATTTCTG CATCTGTAGA TGATAGAACT CGGGAGGAGC GAGGACTGGA GCCATTGAAG 
    AACAATAACT ATAGGATCAG TAAATCCCGA TATGACTCAA TAGACAGCTA TTTATCTAAG 
    TGTGGTGAGA AATATAATGA CATCGACTTG ACGATAGATA AAGAGATCTA CGAACAGCTG 
    TTGCAGGAAG GCATTGATCA TCTCCTGGCC CAGCATGTTG CTCATCTCTT TATTAGAGAC 
    CCACTGACAC TGTTTGAAGA GAAAATACAC CTGGATGATG CTAATGAGTC TGACCATTTT 
    GAGAATATTC AGTCCACAAA TTGGCAGACA ATGAGATTTA AGCCCCCTCC TCCAAACTCA 
    GACATTGGAT GGAGAGTAGA ATTTCGACCC ATGGAGGTGC AATTAACAGA CTTTGAGAAC 
    TCTGCCTATG TGGTGTTTGT GGTACTGCTC ACCAGAGTGA TCCTTTCCTA CAAATTGGAT 
    TTTCTCATTC CACTGTCAAA GGTTGATGAG AACATGAAGG TAGCACAGAA AAGAGATGCT 
    GTCTTGCAGG GAATGTTTTA TTTCAGGAAA GATATTTGCA AAGGTGGCAA TGCAGTGGTG 
    GATGGTTGTG GCAAGGCCCA GAACAGCACG GAGCTCGCTG CAGAGGAGTA CACCCTCATG 
    AGCATAGACA CCATCATCAA TGGGAAGGAA GGTGTGTTTC CTGGACTGAT CCCAATTCTG 
    AACTCTTACC TTGAAAACAT GGAAGTGGAT GTGGACACCA GATGTAGTAT TCTGAACTAC 
    CTAAAGCTAA TTAAGAAGAG AGCATCTGGA GAACTAATGA CAGTTGCCAG ATGGATGAGG 
    GAGTTTATCG CAAACCATCC TGACTACAAG CAAGACAGTG TCATAACTGA TGAAATGAAT 
    TATAGCCTTA TTTTGAAGTG TAACCAAATT GCAAATGAAT TATGTGAATG CCCAGAGTTA 
    CTTGGATCAG CATTTAGGAA AGTAAAATAT AGTGGAAGTA AAACTGACTC ATCCAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001184044.1
    CDS485..2284
    Translation

    Target ORF information:

    RefSeq Version NM_001197115.1
    Organism Homo sapiens(human)
    Definition Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001197115.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AAACCAAGCG CCATGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
    CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
    GAAAATAAAA AAGTCCGGTT GGTCCTGTCT GGGGAGAAAG TTCTTGAAAC TCTGCAAGAG 
    AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTTTGGA GACCAGAGTA TGGGAGTTAC 
    ATGATTGAAG GGACACCAGG ACAGCCCTAC GGAGGAACAA TGTCCGAGTT CAATACAGTT 
    GAGGCCAACA TGCGAAAACG CCGGAAGGAG GCTACTTCTA TATTAGAAGA AAATCAGGCT 
    CTTTGCACAA TAACTTCATT TCCCAGTACC TTAACAAGAA ATATCCGACA TAGGAGAGGA 
    GAAAAGGTTG TCATCAATGT ACCAATATTT AAGGACAAGA ATACACCATC TCCATTTATA 
    GAAACATTTA CTGAGGATGA TGAAGCTTCA AGGGCTTCTA AGCCGGATCA TATTTACATG 
    GATGCCATGG GATTTGGAAT GGGCAATTGC TGTCTCCAGG TGACATTCCA AGCCTGCAGT 
    ATATCTGAGG CCAGATACCT TTATGATCAG TTGGCTACTA TCTGTCCAAT TGTTATGGCT 
    TTGAGTGCTG CATCTCCCTT TTACCGAGGC TATGTGTCAG ACATTGATTG TCGCTGGGGA 
    GTGATTTCTG CATCTGTAGA TGATAGAACT CGGGAGGAGC GAGGACTGGA GCCATTGAAG 
    AACAATAACT ATAGGATCAG TAAATCCCGA TATGACTCAA TAGACAGCTA TTTATCTAAG 
    TGTGGTGAGA AATATAATGA CATCGACTTG ACGATAGATA AAGAGATCTA CGAACAGCTG 
    TTGCAGGAAG GCATTGATCA TCTCCTGGCC CAGCATGTTG CTCATCTCTT TATTAGAGAC 
    CCACTGACAC TGTTTGAAGA GAAAATACAC CTGGATGATG CTAATGAGTC TGACCATTTT 
    GAGAATATTC AGTCCACAAA TTGGCAGACA ATGAGATTTA AGCCCCCTCC TCCAAACTCA 
    GACATTGGAT GGAGAGTAGA ATTTCGACCC ATGGAGGTGC AATTAACAGA CTTTGAGAAC 
    TCTGCCTATG TGGTGTTTGT GGTACTGCTC ACCAGAGTGA TCCTTTCCTA CAAATTGGAT 
    TTTCTCATTC CACTGTCAAA GGTTGATGAG AACATGAAGG TAGCACAGAA AAGAGATGCT 
    GTCTTGCAGG GAATGTTTTA TTTCAGGAAA GATATTTGCA AAGGTGGCAA TGCAGTGGTG 
    GATGGTTGTG GCAAGGCCCA GAACAGCACG GAGCTCGCTG CAGAGGAGTA CACCCTCATG 
    AGCATAGACA CCATCATCAA TGGGAAGGAA GGTGTGTTTC CTGGACTGAT CCCAATTCTG 
    AACTCTTACC TTGAAAACAT GGAAGTGGAT GTGGACACCA GATGTAGTAT TCTGAACTAC 
    CTAAAGCTAA TTAAGAAGAG AGCATCTGGA GAACTAATGA CAGTTGCCAG ATGGATGAGG 
    GAGTTTATCG CAAACCATCC TGACTACAAG CAAGACAGTG TCATAACTGA TGAAATGAAT 
    TATAGCCTTA TTTTGAAGTG TAACCAAATT GCAAATGAAT TATGTGAATG CCCAGAGTTA 
    CTTGGATCAG CATTTAGGAA AGTAAAATAT AGTGGAAGTA AAACTGACTC ATCCAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    GCLC ( NM_001197115.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001184044.1 Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu55651
    Clone ID Related Accession (Same CDS sequence) NM_001197115.1 , NM_001197115.2
    Accession Version NM_001197115.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product glutamate--cysteine ligase catalytic subunit isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI550814.1, AL531593.3, AK313480.1, AB516284.1, AB262176.1 and BC039894.1. On May 31, 2019 this sequence version replaced NM_001197115.1. Transcript Variant: This variant (2, also referred to as delta4) lacks an in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform b), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB516284.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AAACCAAGCG CCATGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
    CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
    GAAAATAAAA AAGTCCGGTT GGTCCTGTCT GGGGAGAAAG TTCTTGAAAC TCTGCAAGAG 
    AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTTTGGA GACCAGAGTA TGGGAGTTAC 
    ATGATTGAAG GGACACCAGG ACAGCCCTAC GGAGGAACAA TGTCCGAGTT CAATACAGTT 
    GAGGCCAACA TGCGAAAACG CCGGAAGGAG GCTACTTCTA TATTAGAAGA AAATCAGGCT 
    CTTTGCACAA TAACTTCATT TCCCAGTACC TTAACAAGAA ATATCCGACA TAGGAGAGGA 
    GAAAAGGTTG TCATCAATGT ACCAATATTT AAGGACAAGA ATACACCATC TCCATTTATA 
    GAAACATTTA CTGAGGATGA TGAAGCTTCA AGGGCTTCTA AGCCGGATCA TATTTACATG 
    GATGCCATGG GATTTGGAAT GGGCAATTGC TGTCTCCAGG TGACATTCCA AGCCTGCAGT 
    ATATCTGAGG CCAGATACCT TTATGATCAG TTGGCTACTA TCTGTCCAAT TGTTATGGCT 
    TTGAGTGCTG CATCTCCCTT TTACCGAGGC TATGTGTCAG ACATTGATTG TCGCTGGGGA 
    GTGATTTCTG CATCTGTAGA TGATAGAACT CGGGAGGAGC GAGGACTGGA GCCATTGAAG 
    AACAATAACT ATAGGATCAG TAAATCCCGA TATGACTCAA TAGACAGCTA TTTATCTAAG 
    TGTGGTGAGA AATATAATGA CATCGACTTG ACGATAGATA AAGAGATCTA CGAACAGCTG 
    TTGCAGGAAG GCATTGATCA TCTCCTGGCC CAGCATGTTG CTCATCTCTT TATTAGAGAC 
    CCACTGACAC TGTTTGAAGA GAAAATACAC CTGGATGATG CTAATGAGTC TGACCATTTT 
    GAGAATATTC AGTCCACAAA TTGGCAGACA ATGAGATTTA AGCCCCCTCC TCCAAACTCA 
    GACATTGGAT GGAGAGTAGA ATTTCGACCC ATGGAGGTGC AATTAACAGA CTTTGAGAAC 
    TCTGCCTATG TGGTGTTTGT GGTACTGCTC ACCAGAGTGA TCCTTTCCTA CAAATTGGAT 
    TTTCTCATTC CACTGTCAAA GGTTGATGAG AACATGAAGG TAGCACAGAA AAGAGATGCT 
    GTCTTGCAGG GAATGTTTTA TTTCAGGAAA GATATTTGCA AAGGTGGCAA TGCAGTGGTG 
    GATGGTTGTG GCAAGGCCCA GAACAGCACG GAGCTCGCTG CAGAGGAGTA CACCCTCATG 
    AGCATAGACA CCATCATCAA TGGGAAGGAA GGTGTGTTTC CTGGACTGAT CCCAATTCTG 
    AACTCTTACC TTGAAAACAT GGAAGTGGAT GTGGACACCA GATGTAGTAT TCTGAACTAC 
    CTAAAGCTAA TTAAGAAGAG AGCATCTGGA GAACTAATGA CAGTTGCCAG ATGGATGAGG 
    GAGTTTATCG CAAACCATCC TGACTACAAG CAAGACAGTG TCATAACTGA TGAAATGAAT 
    TATAGCCTTA TTTTGAAGTG TAACCAAATT GCAAATGAAT TATGTGAATG CCCAGAGTTA 
    CTTGGATCAG CATTTAGGAA AGTAAAATAT AGTGGAAGTA AAACTGACTC ATCCAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001184044.1
    CDS457..2256
    Translation

    Target ORF information:

    RefSeq Version NM_001197115.2
    Organism Homo sapiens(human)
    Definition Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001197115.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AAACCAAGCG CCATGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
    CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
    GAAAATAAAA AAGTCCGGTT GGTCCTGTCT GGGGAGAAAG TTCTTGAAAC TCTGCAAGAG 
    AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTTTGGA GACCAGAGTA TGGGAGTTAC 
    ATGATTGAAG GGACACCAGG ACAGCCCTAC GGAGGAACAA TGTCCGAGTT CAATACAGTT 
    GAGGCCAACA TGCGAAAACG CCGGAAGGAG GCTACTTCTA TATTAGAAGA AAATCAGGCT 
    CTTTGCACAA TAACTTCATT TCCCAGTACC TTAACAAGAA ATATCCGACA TAGGAGAGGA 
    GAAAAGGTTG TCATCAATGT ACCAATATTT AAGGACAAGA ATACACCATC TCCATTTATA 
    GAAACATTTA CTGAGGATGA TGAAGCTTCA AGGGCTTCTA AGCCGGATCA TATTTACATG 
    GATGCCATGG GATTTGGAAT GGGCAATTGC TGTCTCCAGG TGACATTCCA AGCCTGCAGT 
    ATATCTGAGG CCAGATACCT TTATGATCAG TTGGCTACTA TCTGTCCAAT TGTTATGGCT 
    TTGAGTGCTG CATCTCCCTT TTACCGAGGC TATGTGTCAG ACATTGATTG TCGCTGGGGA 
    GTGATTTCTG CATCTGTAGA TGATAGAACT CGGGAGGAGC GAGGACTGGA GCCATTGAAG 
    AACAATAACT ATAGGATCAG TAAATCCCGA TATGACTCAA TAGACAGCTA TTTATCTAAG 
    TGTGGTGAGA AATATAATGA CATCGACTTG ACGATAGATA AAGAGATCTA CGAACAGCTG 
    TTGCAGGAAG GCATTGATCA TCTCCTGGCC CAGCATGTTG CTCATCTCTT TATTAGAGAC 
    CCACTGACAC TGTTTGAAGA GAAAATACAC CTGGATGATG CTAATGAGTC TGACCATTTT 
    GAGAATATTC AGTCCACAAA TTGGCAGACA ATGAGATTTA AGCCCCCTCC TCCAAACTCA 
    GACATTGGAT GGAGAGTAGA ATTTCGACCC ATGGAGGTGC AATTAACAGA CTTTGAGAAC 
    TCTGCCTATG TGGTGTTTGT GGTACTGCTC ACCAGAGTGA TCCTTTCCTA CAAATTGGAT 
    TTTCTCATTC CACTGTCAAA GGTTGATGAG AACATGAAGG TAGCACAGAA AAGAGATGCT 
    GTCTTGCAGG GAATGTTTTA TTTCAGGAAA GATATTTGCA AAGGTGGCAA TGCAGTGGTG 
    GATGGTTGTG GCAAGGCCCA GAACAGCACG GAGCTCGCTG CAGAGGAGTA CACCCTCATG 
    AGCATAGACA CCATCATCAA TGGGAAGGAA GGTGTGTTTC CTGGACTGAT CCCAATTCTG 
    AACTCTTACC TTGAAAACAT GGAAGTGGAT GTGGACACCA GATGTAGTAT TCTGAACTAC 
    CTAAAGCTAA TTAAGAAGAG AGCATCTGGA GAACTAATGA CAGTTGCCAG ATGGATGAGG 
    GAGTTTATCG CAAACCATCC TGACTACAAG CAAGACAGTG TCATAACTGA TGAAATGAAT 
    TATAGCCTTA TTTTGAAGTG TAACCAAATT GCAAATGAAT TATGTGAATG CCCAGAGTTA 
    CTTGGATCAG CATTTAGGAA AGTAAAATAT AGTGGAAGTA AAACTGACTC ATCCAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    GCLC ( XM_017010749.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866238.1 Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OHu96543
    Clone ID Related Accession (Same CDS sequence) XM_017010749.1
    Accession Version XM_017010749.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product glutamate--cysteine ligase catalytic subunit isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGATGCCA TGGGATTTGG AATGGGCAAT TGCTGTCTCC AGGTGACATT CCAAGCCTGC 
    AGTATATCTG AGGCCAGATA CCTTTATGAT CAGTTGGCTA CTATCTGTCC AATTGTTATG 
    GCTTTGAGTG CTGCATCTCC CTTTTACCGA GGCTATGTGT CAGACATTGA TTGTCGCTGG 
    GGAGTGATTT CTGCATCTGT AGATGATAGA ACTCGGGAGG AGCGAGGACT GGAGCCATTG 
    AAGAACAATA ACTATAGGAT CAGTAAATCC CGATATGACT CAATAGACAG CTATTTATCT 
    AAGTGTGGTG AGAAATATAA TGACATCGAC TTGACGATAG ATAAAGAGAT CTACGAACAG 
    CTGTTGCAGG AAGGCATTGA TCATCTCCTG GCCCAGCATG TTGCTCATCT CTTTATTAGA 
    GACCCACTGA CACTGTTTGA AGAGAAAATA CACCTGGATG ATGCTAATGA GTCTGACCAT 
    TTTGAGAATA TTCAGTCCAC AAATTGGCAG ACAATGAGAT TTAAGCCCCC TCCTCCAAAC 
    TCAGACATTG GATGGAGAGT AGAATTTCGA CCCATGGAGG TGCAATTAAC AGACTTTGAG 
    AACTCTGCCT ATGTGGTGTT TGTGGTACTG CTCACCAGAG TGATCCTTTC CTACAAATTG 
    GATTTTCTCA TTCCACTGTC AAAGGTTGAT GAGAACATGA AGGTAGCACA GAAAAGAGAT 
    GCTGTCTTGC AGGGAATGTT TTATTTCAGG AAAGATATTT GCAAAGGTGG CAATGCAGTG 
    GTGGATGGTT GTGGCAAGGC CCAGAACAGC ACGGAGCTCG CTGCAGAGGA GTACACCCTC 
    ATGAGCATAG ACACCATCAT CAATGGGAAG GAAGGTGTGT TTCCTGGACT GATCCCAATT 
    CTGAACTCTT ACCTTGAAAA CATGGAAGTG GATGTGGACA CCAGATGTAG TATTCTGAAC 
    TACCTAAAGC TAATTAAGAA GAGAGCATCT GGAGAACTAA TGACAGTTGC CAGATGGATG 
    AGGGAGTTTA TCGCAAACCA TCCTGACTAC AAGCAAGACA GTGTCATAAC TGATGAAATG 
    AATTATAGCC TTATTTTGAA GTGTAACCAA ATTGCAAATG AATTATGTGA ATGCCCAGAG 
    TTACTTGGAT CAGCATTTAG GAAAGTAAAA TATAGTGGAA GTAAAACTGA CTCATCCAAC 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866238.1
    CDS821..2023
    Translation

    Target ORF information:

    RefSeq Version XM_017010749.1
    Organism Homo sapiens(human)
    Definition Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017010749.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGATGCCA TGGGATTTGG AATGGGCAAT TGCTGTCTCC AGGTGACATT CCAAGCCTGC 
    AGTATATCTG AGGCCAGATA CCTTTATGAT CAGTTGGCTA CTATCTGTCC AATTGTTATG 
    GCTTTGAGTG CTGCATCTCC CTTTTACCGA GGCTATGTGT CAGACATTGA TTGTCGCTGG 
    GGAGTGATTT CTGCATCTGT AGATGATAGA ACTCGGGAGG AGCGAGGACT GGAGCCATTG 
    AAGAACAATA ACTATAGGAT CAGTAAATCC CGATATGACT CAATAGACAG CTATTTATCT 
    AAGTGTGGTG AGAAATATAA TGACATCGAC TTGACGATAG ATAAAGAGAT CTACGAACAG 
    CTGTTGCAGG AAGGCATTGA TCATCTCCTG GCCCAGCATG TTGCTCATCT CTTTATTAGA 
    GACCCACTGA CACTGTTTGA AGAGAAAATA CACCTGGATG ATGCTAATGA GTCTGACCAT 
    TTTGAGAATA TTCAGTCCAC AAATTGGCAG ACAATGAGAT TTAAGCCCCC TCCTCCAAAC 
    TCAGACATTG GATGGAGAGT AGAATTTCGA CCCATGGAGG TGCAATTAAC AGACTTTGAG 
    AACTCTGCCT ATGTGGTGTT TGTGGTACTG CTCACCAGAG TGATCCTTTC CTACAAATTG 
    GATTTTCTCA TTCCACTGTC AAAGGTTGAT GAGAACATGA AGGTAGCACA GAAAAGAGAT 
    GCTGTCTTGC AGGGAATGTT TTATTTCAGG AAAGATATTT GCAAAGGTGG CAATGCAGTG 
    GTGGATGGTT GTGGCAAGGC CCAGAACAGC ACGGAGCTCG CTGCAGAGGA GTACACCCTC 
    ATGAGCATAG ACACCATCAT CAATGGGAAG GAAGGTGTGT TTCCTGGACT GATCCCAATT 
    CTGAACTCTT ACCTTGAAAA CATGGAAGTG GATGTGGACA CCAGATGTAG TATTCTGAAC 
    TACCTAAAGC TAATTAAGAA GAGAGCATCT GGAGAACTAA TGACAGTTGC CAGATGGATG 
    AGGGAGTTTA TCGCAAACCA TCCTGACTAC AAGCAAGACA GTGTCATAAC TGATGAAATG 
    AATTATAGCC TTATTTTGAA GTGTAACCAA ATTGCAAATG AATTATGTGA ATGCCCAGAG 
    TTACTTGGAT CAGCATTTAG GAAAGTAAAA TATAGTGGAA GTAAAACTGA CTCATCCAAC 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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