ORF » Species Summary » Schizosaccharomyces pombe » gcs1 cDNA ORF clone

gcs1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following gcs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gcs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc103598 NM_001018430.2
Latest version!		 Schizosaccharomyces pombe glutamate-cysteine ligase Gcs1 (gcs1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    gcs1 ( NM_001018430.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593031.1 Schizosaccharomyces pombe glutamate-cysteine ligase Gcs1 (gcs1), mRNA.
    Price & Availability↑
    CloneID OSc103598
    Clone ID Related Accession (Same CDS sequence) NM_001018430.2
    Accession Version NM_001018430.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2010bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product glutamate-cysteine ligase Gcs1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018430.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGTTTAT TAGTACTCGG GACTCCTTTA GATTGGCCAG AATCTAAAAA ATATTGTGAT 
    TATGTCCGTG AAAATGGTAT AATGCAATTT TTGCACATGT ATGATACTTA TATATCTAAA 
    AAGCAAGATG TTCTACTTTG GGGGGACGAA ATTGAATGTA TAGTTGTGTC TATGGATGAT 
    AAGAGTAAAA AGGCGCGAGT GAGTCTCCGT CAGGAAGATA TTTTGAACGC CCTCGGTAAA 
    TATGAGGAGA CATTTAGGCA TGTAGATTTT GGACCTGTAT ATGCCGCTTT GAGGAATGAA 
    ACCTGCCCAA AGAAAATAGA CGCCATTTTA TCTGAAGTGG CTAAAAACCC GGCTGATTAT 
    GTCGAACGCA TAGGAGGAAA TTCAAACAAA GATACTATAG AAATTACGAG TTCCACCAAA 
    CCACATGCGC AGAACGCGGT CCCTACTTTC CATCCAGAAT ATGGTCGTTA TATGTTGGAA 
    AGTACACCAG GTGCGCCTTA TGGTTCCACT TTAAAGGATT TTACTTTTGT AGAGTACAAT 
    ATGCGATTAA GAAGAAAAAT TATTGAAAAC CATTTACTAC CCAATGAGCT ACCTTTAACA 
    ATTACTAATT TCTTTCGTCT TGGAACACCA GGCTTTACGG ATCCAGAAGT CGAAGCCAAT 
    GGTGCAATTT CGCGATCTTT TTTCTTACCG GACGATGTTA TCAACACACA TGTTCGTTTC 
    CCGACCTTGA CTGCTAATAT TCGTCAAAGG CGCGGGAGAA AAGTGGCCAT GAATGTTCCG 
    ATTTTCTTCG ATAAAAATAC AATTAAACCA TTTCACGATC CTACCGTTCC TTGGGATAGA 
    AATTTGTTTC CTGAAGATGC AAATGCACGA GATGGTGCAG CGTTAGACAA TCATATTTAC 
    ATGGACAGTA TGGGTTTCGG TATGGGATGC TGTTGTCTAC AAATTACATT CCAAGCTAAA 
    AGTTGTGATG AAGCTAGACT TTTATACGAT CAGTTGACTC CTATTACCCC CTTGATGCTG 
    GCATTATCTG CCGGAACTCC AGCATTTCGA GGCTATTTGG CTGATCAAGA TTGTAGATGG 
    AATGTGATTG CTGGTGCTGT CGATGATCGA ACCGAGGAAG AAATGAAAAC TGTGCCAAAA 
    TCAAGGTATG ATTCTGTTGA CTTATATATC TCGAATGACA AGCGCAATTT ACCGGAATAT 
    AATGACGTCC CGGTCGTTAT AAATCAAGAT TGCTACGATA AGCTAATAAA AGATTGCATT 
    GATGAAAGAT TGGCTAAGCA CATGGCTCAC ATATTCTCTC GTGATCCTTT GGTTATTTTC 
    AGTGACAGCA TTTTACAAGA CAACAGTGTA TCCAATGCTC ATTTTGAGAA CCTAAACTCA 
    ACTAACTGGC AATCTATGCG CTTTAAACCT CCTCCACCAG GTAGTGATAT TGGTTGGCGT 
    GTAGAATTTA GATCAATGGA AATTCAAATA ACTGATTTTG AAAATGCGGC ATATTCAATT 
    TTTGTTGTGA TGCTTTCTCG TGCAATTCTA TCCTTCAATT TGAATCTGTA CATGCCGATA 
    TCGCTAGTGG ATGAAAACAT GAAAGCTGCT CATGCTCGTG ATGCCATTCA TCGTAAAAAG 
    TTTTGGTTCC GTTGCAATCC ATTTCCTGAC GCAAGTACTG ATGATGAATC TGGTCAGTTC 
    CGTCAACTTA CAATTGACGA GTTATTTAAT GGTGAGCACC GTGAAAACGG ATTCCCAGGT 
    TTAATTACCA TTGTTCGAAG CTATTTATAC TCTTGTAATC CTGATGCCAA AACTATTTGC 
    CTAATTGAAC GTTATATTCG CCTAATTTCT CAAAGAGCAA ATGGCCAATG CTTGACTGCT 
    GCTTCGTGGA TACGCAATTT CATTACAACT CATCCTTCTT ATAAGCAAGA CAGCGTGGTC 
    AACGACGAAA TTAATTATGA TTTAATTAGA AGAATAGCAA AAATTGTAGA TGGCGACTAC 
    GACGATACGT TACTTGGAAA ATGCTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_593031.1
    CDS21..2030
    Translation

    Target ORF information:

    RefSeq Version NM_001018430.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe glutamate-cysteine ligase Gcs1 (gcs1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001018430.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGTTTAT TAGTACTCGG GACTCCTTTA GATTGGCCAG AATCTAAAAA ATATTGTGAT 
    TATGTCCGTG AAAATGGTAT AATGCAATTT TTGCACATGT ATGATACTTA TATATCTAAA 
    AAGCAAGATG TTCTACTTTG GGGGGACGAA ATTGAATGTA TAGTTGTGTC TATGGATGAT 
    AAGAGTAAAA AGGCGCGAGT GAGTCTCCGT CAGGAAGATA TTTTGAACGC CCTCGGTAAA 
    TATGAGGAGA CATTTAGGCA TGTAGATTTT GGACCTGTAT ATGCCGCTTT GAGGAATGAA 
    ACCTGCCCAA AGAAAATAGA CGCCATTTTA TCTGAAGTGG CTAAAAACCC GGCTGATTAT 
    GTCGAACGCA TAGGAGGAAA TTCAAACAAA GATACTATAG AAATTACGAG TTCCACCAAA 
    CCACATGCGC AGAACGCGGT CCCTACTTTC CATCCAGAAT ATGGTCGTTA TATGTTGGAA 
    AGTACACCAG GTGCGCCTTA TGGTTCCACT TTAAAGGATT TTACTTTTGT AGAGTACAAT 
    ATGCGATTAA GAAGAAAAAT TATTGAAAAC CATTTACTAC CCAATGAGCT ACCTTTAACA 
    ATTACTAATT TCTTTCGTCT TGGAACACCA GGCTTTACGG ATCCAGAAGT CGAAGCCAAT 
    GGTGCAATTT CGCGATCTTT TTTCTTACCG GACGATGTTA TCAACACACA TGTTCGTTTC 
    CCGACCTTGA CTGCTAATAT TCGTCAAAGG CGCGGGAGAA AAGTGGCCAT GAATGTTCCG 
    ATTTTCTTCG ATAAAAATAC AATTAAACCA TTTCACGATC CTACCGTTCC TTGGGATAGA 
    AATTTGTTTC CTGAAGATGC AAATGCACGA GATGGTGCAG CGTTAGACAA TCATATTTAC 
    ATGGACAGTA TGGGTTTCGG TATGGGATGC TGTTGTCTAC AAATTACATT CCAAGCTAAA 
    AGTTGTGATG AAGCTAGACT TTTATACGAT CAGTTGACTC CTATTACCCC CTTGATGCTG 
    GCATTATCTG CCGGAACTCC AGCATTTCGA GGCTATTTGG CTGATCAAGA TTGTAGATGG 
    AATGTGATTG CTGGTGCTGT CGATGATCGA ACCGAGGAAG AAATGAAAAC TGTGCCAAAA 
    TCAAGGTATG ATTCTGTTGA CTTATATATC TCGAATGACA AGCGCAATTT ACCGGAATAT 
    AATGACGTCC CGGTCGTTAT AAATCAAGAT TGCTACGATA AGCTAATAAA AGATTGCATT 
    GATGAAAGAT TGGCTAAGCA CATGGCTCAC ATATTCTCTC GTGATCCTTT GGTTATTTTC 
    AGTGACAGCA TTTTACAAGA CAACAGTGTA TCCAATGCTC ATTTTGAGAA CCTAAACTCA 
    ACTAACTGGC AATCTATGCG CTTTAAACCT CCTCCACCAG GTAGTGATAT TGGTTGGCGT 
    GTAGAATTTA GATCAATGGA AATTCAAATA ACTGATTTTG AAAATGCGGC ATATTCAATT 
    TTTGTTGTGA TGCTTTCTCG TGCAATTCTA TCCTTCAATT TGAATCTGTA CATGCCGATA 
    TCGCTAGTGG ATGAAAACAT GAAAGCTGCT CATGCTCGTG ATGCCATTCA TCGTAAAAAG 
    TTTTGGTTCC GTTGCAATCC ATTTCCTGAC GCAAGTACTG ATGATGAATC TGGTCAGTTC 
    CGTCAACTTA CAATTGACGA GTTATTTAAT GGTGAGCACC GTGAAAACGG ATTCCCAGGT 
    TTAATTACCA TTGTTCGAAG CTATTTATAC TCTTGTAATC CTGATGCCAA AACTATTTGC 
    CTAATTGAAC GTTATATTCG CCTAATTTCT CAAAGAGCAA ATGGCCAATG CTTGACTGCT 
    GCTTCGTGGA TACGCAATTT CATTACAACT CATCCTTCTT ATAAGCAAGA CAGCGTGGTC 
    AACGACGAAA TTAATTATGA TTTAATTAGA AGAATAGCAA AAATTGTAGA TGGCGACTAC 
    GACGATACGT TACTTGGAAA ATGCTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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