Gclc cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gclc
Entrez Gene ID	14629
Full Name	glutamate-cysteine ligase, catalytic subunit
Synonyms	D9Wsu168e,GLCL-H,Ggcs-hs,Glclc
General protein information	Preferred Names glutamate-cysteine ligase, catalytic subunit Names glutamate--cysteine ligase catalytic subunit GCS heavy chain gamma GCS-HS gamma-ECS gamma-glutamylcysteine synthetase heavy subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 E1\|9 43.36 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006510812.1	XP_006510875.1	glutamate--cysteine ligase catalytic subunit isoform X1
NM_010295.2	NP_034425.1	glutamate--cysteine ligase catalytic subunit

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gclc	NP_036947.1
Saccharomyces cerevisiae (baker's yeast)	GSH1	NP_012434.1
Kluyveromyces lactis	KLLA0F14058g	XP_455711.1
Schizosaccharomyces pombe (fission yeast)	gcs1	NP_593031.1
Magnaporthe oryzae (rice blast fungus)	MGG_07317	XP_003715549.1
Pan troglodytes (chimpanzee)	GCLC	XP_001155930.1
	GCLC	XP_001108943.1
Danio rerio (zebrafish)	gclc	NP_954971.2
Drosophila melanogaster (fruit fly)	Gclc	NP_525005.2
Caenorhabditis elegans (roundworm)	gcs-1	NP_495927.2
Canis lupus familiaris (dog)	GCLC	XP_852845.1
Bos taurus (cattle)	GCLC	NP_001077143.1
Mus musculus (house mouse)	Gclc	NP_034425.1
Gallus gallus (chicken)	GCLC	XP_419910.3
Eremothecium gossypii	AGOS_AFR086C	NP_985633.1
Neurospora crassa	NCU01157	XP_961523.1
Xenopus tropicalis (tropical clawed frog)	gclc	NP_001072803.1
Homo sapiens (human)	GCLC	NP_001489.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001512	XP_321610.3

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptome of the GSH-Depleted Lens Reveals Changes in Detoxification and EMT Signaling Genes, Transport Systems, and Lipid Homeostasis.
Whitson JA, Zhang X, Medvedovic M, Chen J, Wei Z, Monnier VM, Fan X
Investigative ophthalmology & visual science58(5)2666-2684(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

To study the biological effects of low GSH levels, we disrupted its synthesis both at birth by breeding a Gclc loxP mouse with a thy1-cre mouse and at a later age by breeding with a CaMKII-ERT2-Cre (FIGSKO mouse). FIGSKO mice also develop cognitive abnormalities, i.e. learning impairment and nesting behaviors based on passive avoidance, T-Maze, and nesting behavior tests
Title: Gclc deficiency in mouse CNS causes mitochondrial damage and neurodegeneration.

Data show that the catalytic subunit of glutamate cysteine ligase (Gclc)-derived glutathione buffers reactive oxygen species (ROS), and regulates metabolic reprogramming.
Title: Glutathione Primes T Cell Metabolism for Inflammation.

Retinal GCLC was significantly increased in rd10 mice at P21 as well as GSSG. Our results suggest alterations in retinal GCLC content and GSH and/or its precursors in these two RP animal models. Regulation of the enzymes related to GSH metabolism and the retinal concentration of glutamate may be a possible target to delay especially cone death in Retinitis Pigmentosa
Title: Alterations in glutamate cysteine ligase content in the retina of two retinitis pigmentosa animal models.

Clinically relevant levels of TGF-beta1 suppresses GCLC and GCLM expression in mouse lung.
Title: Transforming growth factor ? suppresses glutamate-cysteine ligase gene expression and induces oxidative stress in a lung fibrosis model.

A floxed Gclc mouse was generated and crossed with a transgenic mouse expressing Cre in the lens to generate the Lens Glutathione Synthesis Knockout mouse in which de novo GSH synthesis was completely abolished in the lens.
Title: The LEGSKO mouse: a mouse model of age-related nuclear cataract based on genetic suppression of lens glutathione synthesis.

The following Gclc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gclc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu42544	XM_006510812.1 Latest version!	Mus musculus glutamate-cysteine ligase, catalytic subunit (Gclc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu05905	NM_010295.2 Latest version!	Mus musculus glutamate-cysteine ligase, catalytic subunit (Gclc), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gclc ( XM_006510812.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510875.1 Mus musculus glutamate-cysteine ligase, catalytic subunit (Gclc), transcript variant X1, mRNA.

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CloneID	OMu42544
Clone ID Related Accession (Same CDS sequence)	XM_006510812.1
Accession Version	XM_006510812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	glutamate--cysteine ligase catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTGT CCTTCGATCA TGAAAATAGG AAAGTCCAGT TGTTACTGAA TGGAGGCGAT 
GTTCTTGAGA CTCTGCAGGA GAAGGGGGAG AGGACAAACC CCAACCATCC GACCCTCTGG 
AGACCAGAGT ATGGGAGTTA CATGATCGAA GGAACGCCTG GCCAGCCCTA CGGAGGAACG 
ATGTCTGAGT TCAACACTGT GGAGGCCAAT ATGAGGAAAC GCCGGAAGGA GGCTACTTCT 
GTACTAGGAG AGCATCAGGC TCTCTGCACC ATCACTTCAT TCCCCAGACT AGGCTGTCCT 
GGATTCACAC TGCCAGAACA CAGACCCAAC CCAGAGGAAG GAGGCGCATC AAAGTCCCTC 
TTCTTTCCAG ATGAAGCCAT AAACAAGCAC CCCCGCTTCG GTACTCTAAC AAGAAACATC 
CGGCATCGGA GAGGAGAAAA GGTTGTCATC AATGTGCCAA TATTCAAGGA CAAGAATACA 
CCATCTCCAT TTGTAGAAAC ATTTCCAGAA GATGCGGAGG CATCAAAGGC TTCTCAGCCA 
GACCATATCT ACATGGATGC CATGGGATTC GGGATGGGCA ACTGCTGTCT CCAGGTGACA 
TTCCAAGCCT GCAGCATATC TGAGGCAAGA TACCTTTATG ATCAGCTGGC CACTATCTGC 
CCAATTGTTA TGGCTTTGAG TGCTGCATCT CCATTTTACC GAGGCTACGT GTCAGACATT 
GATTGTCGCT GGGGAGTGAT TTCTGCCTCT GTAGATGATA GAACACGGGA GGAGAGAGGC 
CTGGAGCCTC TGAAGAACAA TCGCTTTAGG ATCAGTAAGT CTCGGTATGA CTCAATAGAT 
AGCTACCTGT CTAAGTGTGG GGAGAAGTAC AATGACATCG ACCTGACCAT CGATAAGGAG 
ATCTACGAGC AGCTCCTGGA GGAAGGCATC GATCATCTTC TGGCACAGCA CGTTGCTCAT 
CTCTTTATTA GAGACCCACT CACCCTTTTT GAAGAGAAAA TACACCTGGA TGATGCCAAC 
GAGTCTGACC ATTTTGAGAA TATTCAGTCT ACAAACTGGC AGACAATGAG GTTTAAGCCT 
CCTCCTCCAA ACTCAGATAT TGGATGGAGA GTAGAGTTCC GACCAATGGA GGTGCAGTTA 
ACAGACTTTG AGAACTCTGC CTATGTGGTA TTCGTGGTAC TGCTCACTAG GGTGATCCTC 
TCATACAAAC TAGACTTCCT CATTCCGCTG TCCAAGGTTG ACGAGAACAT GAAAGTGGCC 
CAGAAGCGAG ATGCTGTCTT GCAGGGGATG TTTTATTTCA GGAAAGACAT CTGCAAAGGC 
GGCAACGCTG TGGTGGACGG GTGCAGCAAG GCCCAGAGCA GCTCTGAGCC AGCTGCAGAG 
GAGTACACCC TGATGAGCAT AGACACCATC ATCAATGGGA AGGAAGGGGT ATTTCCTGGA 
CTCATCCCCA TTCTGAACTC CTATCTGGAG AACATGGAAG TTGATGTGGA CACCCGATGC 
AGTATTCTGA ACTACCTAAA GCTAATTAAG AAGAGAGCAT CTGGAGAACT AATGACTGTT 
GCCAGGTGGA TGAGAGAGTT CATTGCAAAC CATCCTGACT ACAAGCAAGA CAGTGTAATA 
ACTGATGAAA TCAACTATAG CCTCATTTGG AAGTGCAACC AAATTGCAGA TGAGCTGTGT 
GAATGTCCAG AGTTACTTGG ATCAGGATTT AGAAAAGCGA AGTACAGTGG AGGTAAAAGC 
GACCCTTCAG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510875.1
CDS	90..1844
Translation	MLVSFDHENRKVQLLLNGGDVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSVLGEHQALCTITSFPRLGCPGFTLPEHRPNPEEGGASKSLFFPDEAINKHPRFGTLTRNIRHRRGEKVVINVPIFKDKNTPSPFVETFPEDAEASKASQPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNRFRISKSRYDSIDSYLSKCGEKYNDIDLTIDKEIYEQLLEEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQGMFYFRKDICKGGNAVVDGCSKAQSSSEPAAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEINYSLIWKCNQIADELCECPELLGSGFRKAKYSGGKSDPSA

Target ORF information:

RefSeq Version	XM_006510812.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamate-cysteine ligase, catalytic subunit (Gclc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510812.1

ORF Insert Sequence:

ATGTTGGTGT CCTTCGATCA TGAAAATAGG AAAGTCCAGT TGTTACTGAA TGGAGGCGAT 
GTTCTTGAGA CTCTGCAGGA GAAGGGGGAG AGGACAAACC CCAACCATCC GACCCTCTGG 
AGACCAGAGT ATGGGAGTTA CATGATCGAA GGAACGCCTG GCCAGCCCTA CGGAGGAACG 
ATGTCTGAGT TCAACACTGT GGAGGCCAAT ATGAGGAAAC GCCGGAAGGA GGCTACTTCT 
GTACTAGGAG AGCATCAGGC TCTCTGCACC ATCACTTCAT TCCCCAGACT AGGCTGTCCT 
GGATTCACAC TGCCAGAACA CAGACCCAAC CCAGAGGAAG GAGGCGCATC AAAGTCCCTC 
TTCTTTCCAG ATGAAGCCAT AAACAAGCAC CCCCGCTTCG GTACTCTAAC AAGAAACATC 
CGGCATCGGA GAGGAGAAAA GGTTGTCATC AATGTGCCAA TATTCAAGGA CAAGAATACA 
CCATCTCCAT TTGTAGAAAC ATTTCCAGAA GATGCGGAGG CATCAAAGGC TTCTCAGCCA 
GACCATATCT ACATGGATGC CATGGGATTC GGGATGGGCA ACTGCTGTCT CCAGGTGACA 
TTCCAAGCCT GCAGCATATC TGAGGCAAGA TACCTTTATG ATCAGCTGGC CACTATCTGC 
CCAATTGTTA TGGCTTTGAG TGCTGCATCT CCATTTTACC GAGGCTACGT GTCAGACATT 
GATTGTCGCT GGGGAGTGAT TTCTGCCTCT GTAGATGATA GAACACGGGA GGAGAGAGGC 
CTGGAGCCTC TGAAGAACAA TCGCTTTAGG ATCAGTAAGT CTCGGTATGA CTCAATAGAT 
AGCTACCTGT CTAAGTGTGG GGAGAAGTAC AATGACATCG ACCTGACCAT CGATAAGGAG 
ATCTACGAGC AGCTCCTGGA GGAAGGCATC GATCATCTTC TGGCACAGCA CGTTGCTCAT 
CTCTTTATTA GAGACCCACT CACCCTTTTT GAAGAGAAAA TACACCTGGA TGATGCCAAC 
GAGTCTGACC ATTTTGAGAA TATTCAGTCT ACAAACTGGC AGACAATGAG GTTTAAGCCT 
CCTCCTCCAA ACTCAGATAT TGGATGGAGA GTAGAGTTCC GACCAATGGA GGTGCAGTTA 
ACAGACTTTG AGAACTCTGC CTATGTGGTA TTCGTGGTAC TGCTCACTAG GGTGATCCTC 
TCATACAAAC TAGACTTCCT CATTCCGCTG TCCAAGGTTG ACGAGAACAT GAAAGTGGCC 
CAGAAGCGAG ATGCTGTCTT GCAGGGGATG TTTTATTTCA GGAAAGACAT CTGCAAAGGC 
GGCAACGCTG TGGTGGACGG GTGCAGCAAG GCCCAGAGCA GCTCTGAGCC AGCTGCAGAG 
GAGTACACCC TGATGAGCAT AGACACCATC ATCAATGGGA AGGAAGGGGT ATTTCCTGGA 
CTCATCCCCA TTCTGAACTC CTATCTGGAG AACATGGAAG TTGATGTGGA CACCCGATGC 
AGTATTCTGA ACTACCTAAA GCTAATTAAG AAGAGAGCAT CTGGAGAACT AATGACTGTT 
GCCAGGTGGA TGAGAGAGTT CATTGCAAAC CATCCTGACT ACAAGCAAGA CAGTGTAATA 
ACTGATGAAA TCAACTATAG CCTCATTTGG AAGTGCAACC AAATTGCAGA TGAGCTGTGT 
GAATGTCCAG AGTTACTTGG ATCAGGATTT AGAAAAGCGA AGTACAGTGG AGGTAAAAGC 
GACCCTTCAG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gclc ( NM_010295.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034425.1 Mus musculus glutamate-cysteine ligase, catalytic subunit (Gclc), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05905
Clone ID Related Accession (Same CDS sequence)	NM_010295.2
Accession Version	NM_010295.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Mus musculus(house mouse)
Product	glutamate--cysteine ligase catalytic subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK144339.1, AK160876.1 and EH103060.1. On Mar 1, 2011 this sequence version replaced NM_010295.1. Transcript Variant: This variant (1) represents the protein-coding transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.26438.1, AK160876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTGC TGTCCCAAGG CTCGCCACTG AGCTGGGAAG AGACCCAGCG CCACGCCGAC 
CACGTGCGGA GGCACGGCAT CCTCCAGTTC CTGCACATCT ACCACGCAGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGTGACGAG GTGGAGTACA TGTTGGTGTC CTTCGATCAT 
GAAAATAGGA AAGTCCAGTT GTTACTGAAT GGAGGCGATG TTCTTGAGAC TCTGCAGGAG 
AAGGGGGAGA GGACAAACCC CAACCATCCG ACCCTCTGGA GACCAGAGTA TGGGAGTTAC 
ATGATCGAAG GAACGCCTGG CCAGCCCTAC GGAGGAACGA TGTCTGAGTT CAACACTGTG 
GAGGCCAATA TGAGGAAACG CCGGAAGGAG GCTACTTCTG TACTAGGAGA GCATCAGGCT 
CTCTGCACCA TCACTTCATT CCCCAGACTA GGCTGTCCTG GATTCACACT GCCAGAACAC 
AGACCCAACC CAGAGGAAGG AGGCGCATCA AAGTCCCTCT TCTTTCCAGA TGAAGCCATA 
AACAAGCACC CCCGCTTCGG TACTCTAACA AGAAACATCC GGCATCGGAG AGGAGAAAAG 
GTTGTCATCA ATGTGCCAAT ATTCAAGGAC AAGAATACAC CATCTCCATT TGTAGAAACA 
TTTCCAGAAG ATGCGGAGGC ATCAAAGGCT TCTCAGCCAG ACCATATCTA CATGGATGCC 
ATGGGATTCG GGATGGGCAA CTGCTGTCTC CAGGTGACAT TCCAAGCCTG CAGCATATCT 
GAGGCAAGAT ACCTTTATGA TCAGCTGGCC ACTATCTGCC CAATTGTTAT GGCTTTGAGT 
GCTGCATCTC CATTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGAGTGATT 
TCTGCCTCTG TAGATGATAG AACACGGGAG GAGAGAGGCC TGGAGCCTCT GAAGAACAAT 
CGCTTTAGGA TCAGTAAGTC TCGGTATGAC TCAATAGATA GCTACCTGTC TAAGTGTGGG 
GAGAAGTACA ATGACATCGA CCTGACCATC GATAAGGAGA TCTACGAGCA GCTCCTGGAG 
GAAGGCATCG ATCATCTTCT GGCACAGCAC GTTGCTCATC TCTTTATTAG AGACCCACTC 
ACCCTTTTTG AAGAGAAAAT ACACCTGGAT GATGCCAACG AGTCTGACCA TTTTGAGAAT 
ATTCAGTCTA CAAACTGGCA GACAATGAGG TTTAAGCCTC CTCCTCCAAA CTCAGATATT 
GGATGGAGAG TAGAGTTCCG ACCAATGGAG GTGCAGTTAA CAGACTTTGA GAACTCTGCC 
TATGTGGTAT TCGTGGTACT GCTCACTAGG GTGATCCTCT CATACAAACT AGACTTCCTC 
ATTCCGCTGT CCAAGGTTGA CGAGAACATG AAAGTGGCCC AGAAGCGAGA TGCTGTCTTG 
CAGGGGATGT TTTATTTCAG GAAAGACATC TGCAAAGGCG GCAACGCTGT GGTGGACGGG 
TGCAGCAAGG CCCAGAGCAG CTCTGAGCCA GCTGCAGAGG AGTACACCCT GATGAGCATA 
GACACCATCA TCAATGGGAA GGAAGGGGTA TTTCCTGGAC TCATCCCCAT TCTGAACTCC 
TATCTGGAGA ACATGGAAGT TGATGTGGAC ACCCGATGCA GTATTCTGAA CTACCTAAAG 
CTAATTAAGA AGAGAGCATC TGGAGAACTA ATGACTGTTG CCAGGTGGAT GAGAGAGTTC 
ATTGCAAACC ATCCTGACTA CAAGCAAGAC AGTGTAATAA CTGATGAAAT CAACTATAGC 
CTCATTTGGA AGTGCAACCA AATTGCAGAT GAGCTGTGTG AATGTCCAGA GTTACTTGGA 
TCAGGATTTA GAAAAGCGAA GTACAGTGGA GGTAAAAGCG ACCCTTCAGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034425.1
CDS	276..2189
Translation	MGLLSQGSPLSWEETQRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENRKVQLLLNGGDVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSVLGEHQALCTITSFPRLGCPGFTLPEHRPNPEEGGASKSLFFPDEAINKHPRFGTLTRNIRHRRGEKVVINVPIFKDKNTPSPFVETFPEDAEASKASQPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNRFRISKSRYDSIDSYLSKCGEKYNDIDLTIDKEIYEQLLEEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQGMFYFRKDICKGGNAVVDGCSKAQSSSEPAAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEINYSLIWKCNQIADELCECPELLGSGFRKAKYSGGKSDPSA

Target ORF information:

RefSeq Version	NM_010295.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus glutamate-cysteine ligase, catalytic subunit (Gclc), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010295.2

ORF Insert Sequence:

ATGGGGCTGC TGTCCCAAGG CTCGCCACTG AGCTGGGAAG AGACCCAGCG CCACGCCGAC 
CACGTGCGGA GGCACGGCAT CCTCCAGTTC CTGCACATCT ACCACGCAGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGTGACGAG GTGGAGTACA TGTTGGTGTC CTTCGATCAT 
GAAAATAGGA AAGTCCAGTT GTTACTGAAT GGAGGCGATG TTCTTGAGAC TCTGCAGGAG 
AAGGGGGAGA GGACAAACCC CAACCATCCG ACCCTCTGGA GACCAGAGTA TGGGAGTTAC 
ATGATCGAAG GAACGCCTGG CCAGCCCTAC GGAGGAACGA TGTCTGAGTT CAACACTGTG 
GAGGCCAATA TGAGGAAACG CCGGAAGGAG GCTACTTCTG TACTAGGAGA GCATCAGGCT 
CTCTGCACCA TCACTTCATT CCCCAGACTA GGCTGTCCTG GATTCACACT GCCAGAACAC 
AGACCCAACC CAGAGGAAGG AGGCGCATCA AAGTCCCTCT TCTTTCCAGA TGAAGCCATA 
AACAAGCACC CCCGCTTCGG TACTCTAACA AGAAACATCC GGCATCGGAG AGGAGAAAAG 
GTTGTCATCA ATGTGCCAAT ATTCAAGGAC AAGAATACAC CATCTCCATT TGTAGAAACA 
TTTCCAGAAG ATGCGGAGGC ATCAAAGGCT TCTCAGCCAG ACCATATCTA CATGGATGCC 
ATGGGATTCG GGATGGGCAA CTGCTGTCTC CAGGTGACAT TCCAAGCCTG CAGCATATCT 
GAGGCAAGAT ACCTTTATGA TCAGCTGGCC ACTATCTGCC CAATTGTTAT GGCTTTGAGT 
GCTGCATCTC CATTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGAGTGATT 
TCTGCCTCTG TAGATGATAG AACACGGGAG GAGAGAGGCC TGGAGCCTCT GAAGAACAAT 
CGCTTTAGGA TCAGTAAGTC TCGGTATGAC TCAATAGATA GCTACCTGTC TAAGTGTGGG 
GAGAAGTACA ATGACATCGA CCTGACCATC GATAAGGAGA TCTACGAGCA GCTCCTGGAG 
GAAGGCATCG ATCATCTTCT GGCACAGCAC GTTGCTCATC TCTTTATTAG AGACCCACTC 
ACCCTTTTTG AAGAGAAAAT ACACCTGGAT GATGCCAACG AGTCTGACCA TTTTGAGAAT 
ATTCAGTCTA CAAACTGGCA GACAATGAGG TTTAAGCCTC CTCCTCCAAA CTCAGATATT 
GGATGGAGAG TAGAGTTCCG ACCAATGGAG GTGCAGTTAA CAGACTTTGA GAACTCTGCC 
TATGTGGTAT TCGTGGTACT GCTCACTAGG GTGATCCTCT CATACAAACT AGACTTCCTC 
ATTCCGCTGT CCAAGGTTGA CGAGAACATG AAAGTGGCCC AGAAGCGAGA TGCTGTCTTG 
CAGGGGATGT TTTATTTCAG GAAAGACATC TGCAAAGGCG GCAACGCTGT GGTGGACGGG 
TGCAGCAAGG CCCAGAGCAG CTCTGAGCCA GCTGCAGAGG AGTACACCCT GATGAGCATA 
GACACCATCA TCAATGGGAA GGAAGGGGTA TTTCCTGGAC TCATCCCCAT TCTGAACTCC 
TATCTGGAGA ACATGGAAGT TGATGTGGAC ACCCGATGCA GTATTCTGAA CTACCTAAAG 
CTAATTAAGA AGAGAGCATC TGGAGAACTA ATGACTGTTG CCAGGTGGAT GAGAGAGTTC 
ATTGCAAACC ATCCTGACTA CAAGCAAGAC AGTGTAATAA CTGATGAAAT CAACTATAGC 
CTCATTTGGA AGTGCAACCA AATTGCAGAT GAGCTGTGTG AATGTCCAGA GTTACTTGGA 
TCAGGATTTA GAAAAGCGAA GTACAGTGGA GGTAAAAGCG ACCCTTCAGC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptome of the GSH-Depleted Lens Reveals Changes in Detoxification and EMT Signaling Genes, Transport Systems, and Lipid Homeostasis.
Investigative ophthalmology & visual science58(5)2666-2684(2017 May)
Whitson JA,Zhang X,Medvedovic M,Chen J,Wei Z,Monnier VM,Fan X

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Gclc cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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