GCLC cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCLC
Entrez Gene ID	2729
Full Name	glutamate-cysteine ligase catalytic subunit
Synonyms	GCL,GCS,GLCL,GLCLC
General protein information	Preferred Names glutamate-cysteine ligase catalytic subunit Names glutamate--cysteine ligase catalytic subunit GCS heavy chain gamma-ECS gamma-glutamylcysteine synthetase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p12.1
Summary	Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010].
Disorder MIM:	606857
Disorder Html:	Hemolytic anemia due to gamma-glutamylcysteine synthetase deficiency, 230450 (3); {Myocardial infarction, susceptibility to}, 608446 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001498.3	NP_001489.1	glutamate--cysteine ligase catalytic subunit isoform a
XM_017010749.1	XP_016866238.1	glutamate--cysteine ligase catalytic subunit isoform X1
NM_001197115.1	NP_001184044.1	glutamate--cysteine ligase catalytic subunit isoform b
NM_001498.4	NP_001489.1	glutamate--cysteine ligase catalytic subunit isoform a
NM_001197115.2	NP_001184044.1	glutamate--cysteine ligase catalytic subunit isoform b

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gclc	NP_036947.1
Saccharomyces cerevisiae (baker's yeast)	GSH1	NP_012434.1
Kluyveromyces lactis	KLLA0F14058g	XP_455711.1
Schizosaccharomyces pombe (fission yeast)	gcs1	NP_593031.1
Magnaporthe oryzae (rice blast fungus)	MGG_07317	XP_003715549.1
Pan troglodytes (chimpanzee)	GCLC	XP_001155930.1
	GCLC	XP_001108943.1
Danio rerio (zebrafish)	gclc	NP_954971.2
Drosophila melanogaster (fruit fly)	Gclc	NP_525005.2
Caenorhabditis elegans (roundworm)	gcs-1	NP_495927.2
Canis lupus familiaris (dog)	GCLC	XP_852845.1
Bos taurus (cattle)	GCLC	NP_001077143.1
Mus musculus (house mouse)	Gclc	NP_034425.1
Gallus gallus (chicken)	GCLC	XP_419910.3
Eremothecium gossypii	AGOS_AFR086C	NP_985633.1
Neurospora crassa	NCU01157	XP_961523.1
Xenopus tropicalis (tropical clawed frog)	gclc	NP_001072803.1
Homo sapiens (human)	GCLC	NP_001489.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001512	XP_321610.3

Gene Ontology
Bibliography

Related articles in PubMed

Transformation of liver cells by 3-methylcholanthrene potentiates oxidative stress via the downregulation of glutathione synthesis.
Kim SH, Yoon D, Lee YH, Lee J, Kim ND, Kim S, Jung YS
International journal of molecular medicine40(6)2011-2017(2017 Dec)

Polymorphism of Glutamate-Cysteine Ligase Subunit Catalytic (GCLC) Gene in Pulmonary Tuberculosis Patients.
Yuniastuti A, Susanti R, Mustikaningtyas D
Pakistan journal of biological sciences : PJBS20(8)397-402(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of CD44 reduced the protein level of xCT, a cystine transporter, and increased oxidative stress. However, an increase in GSH was also observed and was associated with enhanced chemoresistance in CD44-knockdown cells. Increased GSH was mediated by the Nrf2/AP-1-induced upregulation of GCLC, a subunit of the enzyme catalyzing GSH synthesis
Title: Rheostatic CD44 isoform expression and its association with oxidative stress in human malignant mesothelioma.

NQO1 and GCLC were both functionally sufficient to autonomously confer a tamoxifen-resistant metabolic phenotype, characterized by i) increased mitochondrial biogenesis, ii) increased ATP production and iii) reduced glutathione levels.
Title: Mitochondrial "power" drives tamoxifen resistance: NQO1 and GCLC are new therapeutic targets in breast cancer.

GCLC polymorphisms correlated with brain GSH and Glu levels in psychosis.
Title: Genetic Polymorphism Associated Prefrontal Glutathione and Its Coupling With Brain Glutamate and Peripheral Redox Status in Early Psychosis.

(i) melatonin counteracted UVR-induced alterations in the ATP synthesis and reduced free radical formation; (ii) melatonin induced the translocation of Nrf2 transcription factor from the cytosol into the nucleus resulting in, (iii) melatonin enhanced gene expression of phase-2 antioxidative enzymes including gamma-glutamylcysteine synthetase (gamma-GCS), heme oxygenase-1 (HO-1), and NADPH: quinone dehydrogenase-1 (NQO1...
Title: Melatonin enhances mitochondrial ATP synthesis, reduces reactive oxygen species formation, and mediates translocation of the nuclear erythroid 2-related factor 2 resulting in activation of phase-2 antioxidant enzymes (?-GCS, HO-1, NQO1) in ultraviolet radiation-treated normal human epidermal keratinocytes (NHEK).

Glutaminolysis is activated in ES2 and OVCAR3, though ES2 exclusively synthesizes amino acids and GSH. ES2 cells are more resistant to carboplatin than OVCAR3 and the abrogation of GSH production by BSO sensitizes ES2 to carboplatin. HNF1beta regulates the expression of GCLC, but not GCLM, and consequently GSH production in ES2
Title: HNF1? drives glutathione (GSH) synthesis underlying intrinsic carboplatin resistance of ovarian clear cell carcinoma (OCCC).

The following GCLC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCLC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18571	NM_001498.4 Latest version!	Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu55651	NM_001197115.2 Latest version!	Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00
OHu96543	XM_017010749.1 Latest version!	Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu18571	NM_001498.3	Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu55651	NM_001197115.1	Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCLC ( NM_001498.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001489.1 Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18571
Clone ID Related Accession (Same CDS sequence)	NM_001498.3 , NM_001498.4
Accession Version	NM_001498.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	glutamate--cysteine ligase catalytic subunit isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI550814.1, AL531593.3, AK313480.1, AB262176.1 and BC039894.1. This sequence is a reference standard in the RefSeqGene project. On Oct 7, 2010 this sequence version replaced NM_001498.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC039894.1, AB262176.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AAACCAAGCG CCATGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
GAAAATAAAA AAGTCCGGTT GGTCCTGTCT GGGGAGAAAG TTCTTGAAAC TCTGCAAGAG 
AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTTTGGA GACCAGAGTA TGGGAGTTAC 
ATGATTGAAG GGACACCAGG ACAGCCCTAC GGAGGAACAA TGTCCGAGTT CAATACAGTT 
GAGGCCAACA TGCGAAAACG CCGGAAGGAG GCTACTTCTA TATTAGAAGA AAATCAGGCT 
CTTTGCACAA TAACTTCATT TCCCAGATTA GGCTGTCCTG GGTTCACACT GCCCGAGGTC 
AAACCCAACC CAGTGGAAGG AGGAGCTTCC AAGTCCCTCT TCTTTCCAGA TGAAGCAATA 
AACAAGCACC CTCGCTTCAG TACCTTAACA AGAAATATCC GACATAGGAG AGGAGAAAAG 
GTTGTCATCA ATGTACCAAT ATTTAAGGAC AAGAATACAC CATCTCCATT TATAGAAACA 
TTTACTGAGG ATGATGAAGC TTCAAGGGCT TCTAAGCCGG ATCATATTTA CATGGATGCC 
ATGGGATTTG GAATGGGCAA TTGCTGTCTC CAGGTGACAT TCCAAGCCTG CAGTATATCT 
GAGGCCAGAT ACCTTTATGA TCAGTTGGCT ACTATCTGTC CAATTGTTAT GGCTTTGAGT 
GCTGCATCTC CCTTTTACCG AGGCTATGTG TCAGACATTG ATTGTCGCTG GGGAGTGATT 
TCTGCATCTG TAGATGATAG AACTCGGGAG GAGCGAGGAC TGGAGCCATT GAAGAACAAT 
AACTATAGGA TCAGTAAATC CCGATATGAC TCAATAGACA GCTATTTATC TAAGTGTGGT 
GAGAAATATA ATGACATCGA CTTGACGATA GATAAAGAGA TCTACGAACA GCTGTTGCAG 
GAAGGCATTG ATCATCTCCT GGCCCAGCAT GTTGCTCATC TCTTTATTAG AGACCCACTG 
ACACTGTTTG AAGAGAAAAT ACACCTGGAT GATGCTAATG AGTCTGACCA TTTTGAGAAT 
ATTCAGTCCA CAAATTGGCA GACAATGAGA TTTAAGCCCC CTCCTCCAAA CTCAGACATT 
GGATGGAGAG TAGAATTTCG ACCCATGGAG GTGCAATTAA CAGACTTTGA GAACTCTGCC 
TATGTGGTGT TTGTGGTACT GCTCACCAGA GTGATCCTTT CCTACAAATT GGATTTTCTC 
ATTCCACTGT CAAAGGTTGA TGAGAACATG AAGGTAGCAC AGAAAAGAGA TGCTGTCTTG 
CAGGGAATGT TTTATTTCAG GAAAGATATT TGCAAAGGTG GCAATGCAGT GGTGGATGGT 
TGTGGCAAGG CCCAGAACAG CACGGAGCTC GCTGCAGAGG AGTACACCCT CATGAGCATA 
GACACCATCA TCAATGGGAA GGAAGGTGTG TTTCCTGGAC TGATCCCAAT TCTGAACTCT 
TACCTTGAAA ACATGGAAGT GGATGTGGAC ACCAGATGTA GTATTCTGAA CTACCTAAAG 
CTAATTAAGA AGAGAGCATC TGGAGAACTA ATGACAGTTG CCAGATGGAT GAGGGAGTTT 
ATCGCAAACC ATCCTGACTA CAAGCAAGAC AGTGTCATAA CTGATGAAAT GAATTATAGC 
CTTATTTTGA AGTGTAACCA AATTGCAAAT GAATTATGTG AATGCCCAGA GTTACTTGGA 
TCAGCATTTA GGAAAGTAAA ATATAGTGGA AGTAAAACTG ACTCATCCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001489.1
CDS	485..2398
Translation	MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEVKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNNYRISKSRYDSIDSYLSKCGEKYNDIDLTIDKEIYEQLLQEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQGMFYFRKDICKGGNAVVDGCGKAQNSTELAAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELLGSAFRKVKYSGSKTDSSN

Target ORF information:

RefSeq Version	NM_001498.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001498.3

ORF Insert Sequence:

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AAACCAAGCG CCATGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
GAAAATAAAA AAGTCCGGTT GGTCCTGTCT GGGGAGAAAG TTCTTGAAAC TCTGCAAGAG 
AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTTTGGA GACCAGAGTA TGGGAGTTAC 
ATGATTGAAG GGACACCAGG ACAGCCCTAC GGAGGAACAA TGTCCGAGTT CAATACAGTT 
GAGGCCAACA TGCGAAAACG CCGGAAGGAG GCTACTTCTA TATTAGAAGA AAATCAGGCT 
CTTTGCACAA TAACTTCATT TCCCAGATTA GGCTGTCCTG GGTTCACACT GCCCGAGGTC 
AAACCCAACC CAGTGGAAGG AGGAGCTTCC AAGTCCCTCT TCTTTCCAGA TGAAGCAATA 
AACAAGCACC CTCGCTTCAG TACCTTAACA AGAAATATCC GACATAGGAG AGGAGAAAAG 
GTTGTCATCA ATGTACCAAT ATTTAAGGAC AAGAATACAC CATCTCCATT TATAGAAACA 
TTTACTGAGG ATGATGAAGC TTCAAGGGCT TCTAAGCCGG ATCATATTTA CATGGATGCC 
ATGGGATTTG GAATGGGCAA TTGCTGTCTC CAGGTGACAT TCCAAGCCTG CAGTATATCT 
GAGGCCAGAT ACCTTTATGA TCAGTTGGCT ACTATCTGTC CAATTGTTAT GGCTTTGAGT 
GCTGCATCTC CCTTTTACCG AGGCTATGTG TCAGACATTG ATTGTCGCTG GGGAGTGATT 
TCTGCATCTG TAGATGATAG AACTCGGGAG GAGCGAGGAC TGGAGCCATT GAAGAACAAT 
AACTATAGGA TCAGTAAATC CCGATATGAC TCAATAGACA GCTATTTATC TAAGTGTGGT 
GAGAAATATA ATGACATCGA CTTGACGATA GATAAAGAGA TCTACGAACA GCTGTTGCAG 
GAAGGCATTG ATCATCTCCT GGCCCAGCAT GTTGCTCATC TCTTTATTAG AGACCCACTG 
ACACTGTTTG AAGAGAAAAT ACACCTGGAT GATGCTAATG AGTCTGACCA TTTTGAGAAT 
ATTCAGTCCA CAAATTGGCA GACAATGAGA TTTAAGCCCC CTCCTCCAAA CTCAGACATT 
GGATGGAGAG TAGAATTTCG ACCCATGGAG GTGCAATTAA CAGACTTTGA GAACTCTGCC 
TATGTGGTGT TTGTGGTACT GCTCACCAGA GTGATCCTTT CCTACAAATT GGATTTTCTC 
ATTCCACTGT CAAAGGTTGA TGAGAACATG AAGGTAGCAC AGAAAAGAGA TGCTGTCTTG 
CAGGGAATGT TTTATTTCAG GAAAGATATT TGCAAAGGTG GCAATGCAGT GGTGGATGGT 
TGTGGCAAGG CCCAGAACAG CACGGAGCTC GCTGCAGAGG AGTACACCCT CATGAGCATA 
GACACCATCA TCAATGGGAA GGAAGGTGTG TTTCCTGGAC TGATCCCAAT TCTGAACTCT 
TACCTTGAAA ACATGGAAGT GGATGTGGAC ACCAGATGTA GTATTCTGAA CTACCTAAAG 
CTAATTAAGA AGAGAGCATC TGGAGAACTA ATGACAGTTG CCAGATGGAT GAGGGAGTTT 
ATCGCAAACC ATCCTGACTA CAAGCAAGAC AGTGTCATAA CTGATGAAAT GAATTATAGC 
CTTATTTTGA AGTGTAACCA AATTGCAAAT GAATTATGTG AATGCCCAGA GTTACTTGGA 
TCAGCATTTA GGAAAGTAAA ATATAGTGGA AGTAAAACTG ACTCATCCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLC ( NM_001498.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001489.1 Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18571
Clone ID Related Accession (Same CDS sequence)	NM_001498.3 , NM_001498.4
Accession Version	NM_001498.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	glutamate--cysteine ligase catalytic subunit isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI550814.1, AL531593.3, AK313480.1, AB262176.1 and BC039894.1. This sequence is a reference standard in the RefSeqGene project. On May 8, 2018 this sequence version replaced NM_001498.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.95345.1, AB262176.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000650454.1/ ENSP00000497574.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AAACCAAGCG CCATGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
GAAAATAAAA AAGTCCGGTT GGTCCTGTCT GGGGAGAAAG TTCTTGAAAC TCTGCAAGAG 
AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTTTGGA GACCAGAGTA TGGGAGTTAC 
ATGATTGAAG GGACACCAGG ACAGCCCTAC GGAGGAACAA TGTCCGAGTT CAATACAGTT 
GAGGCCAACA TGCGAAAACG CCGGAAGGAG GCTACTTCTA TATTAGAAGA AAATCAGGCT 
CTTTGCACAA TAACTTCATT TCCCAGATTA GGCTGTCCTG GGTTCACACT GCCCGAGGTC 
AAACCCAACC CAGTGGAAGG AGGAGCTTCC AAGTCCCTCT TCTTTCCAGA TGAAGCAATA 
AACAAGCACC CTCGCTTCAG TACCTTAACA AGAAATATCC GACATAGGAG AGGAGAAAAG 
GTTGTCATCA ATGTACCAAT ATTTAAGGAC AAGAATACAC CATCTCCATT TATAGAAACA 
TTTACTGAGG ATGATGAAGC TTCAAGGGCT TCTAAGCCGG ATCATATTTA CATGGATGCC 
ATGGGATTTG GAATGGGCAA TTGCTGTCTC CAGGTGACAT TCCAAGCCTG CAGTATATCT 
GAGGCCAGAT ACCTTTATGA TCAGTTGGCT ACTATCTGTC CAATTGTTAT GGCTTTGAGT 
GCTGCATCTC CCTTTTACCG AGGCTATGTG TCAGACATTG ATTGTCGCTG GGGAGTGATT 
TCTGCATCTG TAGATGATAG AACTCGGGAG GAGCGAGGAC TGGAGCCATT GAAGAACAAT 
AACTATAGGA TCAGTAAATC CCGATATGAC TCAATAGACA GCTATTTATC TAAGTGTGGT 
GAGAAATATA ATGACATCGA CTTGACGATA GATAAAGAGA TCTACGAACA GCTGTTGCAG 
GAAGGCATTG ATCATCTCCT GGCCCAGCAT GTTGCTCATC TCTTTATTAG AGACCCACTG 
ACACTGTTTG AAGAGAAAAT ACACCTGGAT GATGCTAATG AGTCTGACCA TTTTGAGAAT 
ATTCAGTCCA CAAATTGGCA GACAATGAGA TTTAAGCCCC CTCCTCCAAA CTCAGACATT 
GGATGGAGAG TAGAATTTCG ACCCATGGAG GTGCAATTAA CAGACTTTGA GAACTCTGCC 
TATGTGGTGT TTGTGGTACT GCTCACCAGA GTGATCCTTT CCTACAAATT GGATTTTCTC 
ATTCCACTGT CAAAGGTTGA TGAGAACATG AAGGTAGCAC AGAAAAGAGA TGCTGTCTTG 
CAGGGAATGT TTTATTTCAG GAAAGATATT TGCAAAGGTG GCAATGCAGT GGTGGATGGT 
TGTGGCAAGG CCCAGAACAG CACGGAGCTC GCTGCAGAGG AGTACACCCT CATGAGCATA 
GACACCATCA TCAATGGGAA GGAAGGTGTG TTTCCTGGAC TGATCCCAAT TCTGAACTCT 
TACCTTGAAA ACATGGAAGT GGATGTGGAC ACCAGATGTA GTATTCTGAA CTACCTAAAG 
CTAATTAAGA AGAGAGCATC TGGAGAACTA ATGACAGTTG CCAGATGGAT GAGGGAGTTT 
ATCGCAAACC ATCCTGACTA CAAGCAAGAC AGTGTCATAA CTGATGAAAT GAATTATAGC 
CTTATTTTGA AGTGTAACCA AATTGCAAAT GAATTATGTG AATGCCCAGA GTTACTTGGA 
TCAGCATTTA GGAAAGTAAA ATATAGTGGA AGTAAAACTG ACTCATCCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001489.1
CDS	457..2370
Translation	MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEVKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNNYRISKSRYDSIDSYLSKCGEKYNDIDLTIDKEIYEQLLQEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQGMFYFRKDICKGGNAVVDGCGKAQNSTELAAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELLGSAFRKVKYSGSKTDSSN

Target ORF information:

RefSeq Version	NM_001498.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001498.4

ORF Insert Sequence:

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AAACCAAGCG CCATGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
GAAAATAAAA AAGTCCGGTT GGTCCTGTCT GGGGAGAAAG TTCTTGAAAC TCTGCAAGAG 
AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTTTGGA GACCAGAGTA TGGGAGTTAC 
ATGATTGAAG GGACACCAGG ACAGCCCTAC GGAGGAACAA TGTCCGAGTT CAATACAGTT 
GAGGCCAACA TGCGAAAACG CCGGAAGGAG GCTACTTCTA TATTAGAAGA AAATCAGGCT 
CTTTGCACAA TAACTTCATT TCCCAGATTA GGCTGTCCTG GGTTCACACT GCCCGAGGTC 
AAACCCAACC CAGTGGAAGG AGGAGCTTCC AAGTCCCTCT TCTTTCCAGA TGAAGCAATA 
AACAAGCACC CTCGCTTCAG TACCTTAACA AGAAATATCC GACATAGGAG AGGAGAAAAG 
GTTGTCATCA ATGTACCAAT ATTTAAGGAC AAGAATACAC CATCTCCATT TATAGAAACA 
TTTACTGAGG ATGATGAAGC TTCAAGGGCT TCTAAGCCGG ATCATATTTA CATGGATGCC 
ATGGGATTTG GAATGGGCAA TTGCTGTCTC CAGGTGACAT TCCAAGCCTG CAGTATATCT 
GAGGCCAGAT ACCTTTATGA TCAGTTGGCT ACTATCTGTC CAATTGTTAT GGCTTTGAGT 
GCTGCATCTC CCTTTTACCG AGGCTATGTG TCAGACATTG ATTGTCGCTG GGGAGTGATT 
TCTGCATCTG TAGATGATAG AACTCGGGAG GAGCGAGGAC TGGAGCCATT GAAGAACAAT 
AACTATAGGA TCAGTAAATC CCGATATGAC TCAATAGACA GCTATTTATC TAAGTGTGGT 
GAGAAATATA ATGACATCGA CTTGACGATA GATAAAGAGA TCTACGAACA GCTGTTGCAG 
GAAGGCATTG ATCATCTCCT GGCCCAGCAT GTTGCTCATC TCTTTATTAG AGACCCACTG 
ACACTGTTTG AAGAGAAAAT ACACCTGGAT GATGCTAATG AGTCTGACCA TTTTGAGAAT 
ATTCAGTCCA CAAATTGGCA GACAATGAGA TTTAAGCCCC CTCCTCCAAA CTCAGACATT 
GGATGGAGAG TAGAATTTCG ACCCATGGAG GTGCAATTAA CAGACTTTGA GAACTCTGCC 
TATGTGGTGT TTGTGGTACT GCTCACCAGA GTGATCCTTT CCTACAAATT GGATTTTCTC 
ATTCCACTGT CAAAGGTTGA TGAGAACATG AAGGTAGCAC AGAAAAGAGA TGCTGTCTTG 
CAGGGAATGT TTTATTTCAG GAAAGATATT TGCAAAGGTG GCAATGCAGT GGTGGATGGT 
TGTGGCAAGG CCCAGAACAG CACGGAGCTC GCTGCAGAGG AGTACACCCT CATGAGCATA 
GACACCATCA TCAATGGGAA GGAAGGTGTG TTTCCTGGAC TGATCCCAAT TCTGAACTCT 
TACCTTGAAA ACATGGAAGT GGATGTGGAC ACCAGATGTA GTATTCTGAA CTACCTAAAG 
CTAATTAAGA AGAGAGCATC TGGAGAACTA ATGACAGTTG CCAGATGGAT GAGGGAGTTT 
ATCGCAAACC ATCCTGACTA CAAGCAAGAC AGTGTCATAA CTGATGAAAT GAATTATAGC 
CTTATTTTGA AGTGTAACCA AATTGCAAAT GAATTATGTG AATGCCCAGA GTTACTTGGA 
TCAGCATTTA GGAAAGTAAA ATATAGTGGA AGTAAAACTG ACTCATCCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLC ( NM_001197115.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001184044.1 Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 2, mRNA.

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CloneID	OHu55651
Clone ID Related Accession (Same CDS sequence)	NM_001197115.1 , NM_001197115.2
Accession Version	NM_001197115.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	glutamate--cysteine ligase catalytic subunit isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI550814.1, AL531593.3, AK313480.1, AB516284.1, AB262176.1 and BC039894.1. Transcript Variant: This variant (2, also referred to as delta4) lacks an in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform b), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB516284.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AAACCAAGCG CCATGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
GAAAATAAAA AAGTCCGGTT GGTCCTGTCT GGGGAGAAAG TTCTTGAAAC TCTGCAAGAG 
AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTTTGGA GACCAGAGTA TGGGAGTTAC 
ATGATTGAAG GGACACCAGG ACAGCCCTAC GGAGGAACAA TGTCCGAGTT CAATACAGTT 
GAGGCCAACA TGCGAAAACG CCGGAAGGAG GCTACTTCTA TATTAGAAGA AAATCAGGCT 
CTTTGCACAA TAACTTCATT TCCCAGTACC TTAACAAGAA ATATCCGACA TAGGAGAGGA 
GAAAAGGTTG TCATCAATGT ACCAATATTT AAGGACAAGA ATACACCATC TCCATTTATA 
GAAACATTTA CTGAGGATGA TGAAGCTTCA AGGGCTTCTA AGCCGGATCA TATTTACATG 
GATGCCATGG GATTTGGAAT GGGCAATTGC TGTCTCCAGG TGACATTCCA AGCCTGCAGT 
ATATCTGAGG CCAGATACCT TTATGATCAG TTGGCTACTA TCTGTCCAAT TGTTATGGCT 
TTGAGTGCTG CATCTCCCTT TTACCGAGGC TATGTGTCAG ACATTGATTG TCGCTGGGGA 
GTGATTTCTG CATCTGTAGA TGATAGAACT CGGGAGGAGC GAGGACTGGA GCCATTGAAG 
AACAATAACT ATAGGATCAG TAAATCCCGA TATGACTCAA TAGACAGCTA TTTATCTAAG 
TGTGGTGAGA AATATAATGA CATCGACTTG ACGATAGATA AAGAGATCTA CGAACAGCTG 
TTGCAGGAAG GCATTGATCA TCTCCTGGCC CAGCATGTTG CTCATCTCTT TATTAGAGAC 
CCACTGACAC TGTTTGAAGA GAAAATACAC CTGGATGATG CTAATGAGTC TGACCATTTT 
GAGAATATTC AGTCCACAAA TTGGCAGACA ATGAGATTTA AGCCCCCTCC TCCAAACTCA 
GACATTGGAT GGAGAGTAGA ATTTCGACCC ATGGAGGTGC AATTAACAGA CTTTGAGAAC 
TCTGCCTATG TGGTGTTTGT GGTACTGCTC ACCAGAGTGA TCCTTTCCTA CAAATTGGAT 
TTTCTCATTC CACTGTCAAA GGTTGATGAG AACATGAAGG TAGCACAGAA AAGAGATGCT 
GTCTTGCAGG GAATGTTTTA TTTCAGGAAA GATATTTGCA AAGGTGGCAA TGCAGTGGTG 
GATGGTTGTG GCAAGGCCCA GAACAGCACG GAGCTCGCTG CAGAGGAGTA CACCCTCATG 
AGCATAGACA CCATCATCAA TGGGAAGGAA GGTGTGTTTC CTGGACTGAT CCCAATTCTG 
AACTCTTACC TTGAAAACAT GGAAGTGGAT GTGGACACCA GATGTAGTAT TCTGAACTAC 
CTAAAGCTAA TTAAGAAGAG AGCATCTGGA GAACTAATGA CAGTTGCCAG ATGGATGAGG 
GAGTTTATCG CAAACCATCC TGACTACAAG CAAGACAGTG TCATAACTGA TGAAATGAAT 
TATAGCCTTA TTTTGAAGTG TAACCAAATT GCAAATGAAT TATGTGAATG CCCAGAGTTA 
CTTGGATCAG CATTTAGGAA AGTAAAATAT AGTGGAAGTA AAACTGACTC ATCCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001184044.1
CDS	485..2284
Translation	MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNNYRISKSRYDSIDSYLSKCGEKYNDIDLTIDKEIYEQLLQEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQGMFYFRKDICKGGNAVVDGCGKAQNSTELAAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELLGSAFRKVKYSGSKTDSSN

Target ORF information:

RefSeq Version	NM_001197115.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001197115.1

ORF Insert Sequence:

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AAACCAAGCG CCATGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
GAAAATAAAA AAGTCCGGTT GGTCCTGTCT GGGGAGAAAG TTCTTGAAAC TCTGCAAGAG 
AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTTTGGA GACCAGAGTA TGGGAGTTAC 
ATGATTGAAG GGACACCAGG ACAGCCCTAC GGAGGAACAA TGTCCGAGTT CAATACAGTT 
GAGGCCAACA TGCGAAAACG CCGGAAGGAG GCTACTTCTA TATTAGAAGA AAATCAGGCT 
CTTTGCACAA TAACTTCATT TCCCAGTACC TTAACAAGAA ATATCCGACA TAGGAGAGGA 
GAAAAGGTTG TCATCAATGT ACCAATATTT AAGGACAAGA ATACACCATC TCCATTTATA 
GAAACATTTA CTGAGGATGA TGAAGCTTCA AGGGCTTCTA AGCCGGATCA TATTTACATG 
GATGCCATGG GATTTGGAAT GGGCAATTGC TGTCTCCAGG TGACATTCCA AGCCTGCAGT 
ATATCTGAGG CCAGATACCT TTATGATCAG TTGGCTACTA TCTGTCCAAT TGTTATGGCT 
TTGAGTGCTG CATCTCCCTT TTACCGAGGC TATGTGTCAG ACATTGATTG TCGCTGGGGA 
GTGATTTCTG CATCTGTAGA TGATAGAACT CGGGAGGAGC GAGGACTGGA GCCATTGAAG 
AACAATAACT ATAGGATCAG TAAATCCCGA TATGACTCAA TAGACAGCTA TTTATCTAAG 
TGTGGTGAGA AATATAATGA CATCGACTTG ACGATAGATA AAGAGATCTA CGAACAGCTG 
TTGCAGGAAG GCATTGATCA TCTCCTGGCC CAGCATGTTG CTCATCTCTT TATTAGAGAC 
CCACTGACAC TGTTTGAAGA GAAAATACAC CTGGATGATG CTAATGAGTC TGACCATTTT 
GAGAATATTC AGTCCACAAA TTGGCAGACA ATGAGATTTA AGCCCCCTCC TCCAAACTCA 
GACATTGGAT GGAGAGTAGA ATTTCGACCC ATGGAGGTGC AATTAACAGA CTTTGAGAAC 
TCTGCCTATG TGGTGTTTGT GGTACTGCTC ACCAGAGTGA TCCTTTCCTA CAAATTGGAT 
TTTCTCATTC CACTGTCAAA GGTTGATGAG AACATGAAGG TAGCACAGAA AAGAGATGCT 
GTCTTGCAGG GAATGTTTTA TTTCAGGAAA GATATTTGCA AAGGTGGCAA TGCAGTGGTG 
GATGGTTGTG GCAAGGCCCA GAACAGCACG GAGCTCGCTG CAGAGGAGTA CACCCTCATG 
AGCATAGACA CCATCATCAA TGGGAAGGAA GGTGTGTTTC CTGGACTGAT CCCAATTCTG 
AACTCTTACC TTGAAAACAT GGAAGTGGAT GTGGACACCA GATGTAGTAT TCTGAACTAC 
CTAAAGCTAA TTAAGAAGAG AGCATCTGGA GAACTAATGA CAGTTGCCAG ATGGATGAGG 
GAGTTTATCG CAAACCATCC TGACTACAAG CAAGACAGTG TCATAACTGA TGAAATGAAT 
TATAGCCTTA TTTTGAAGTG TAACCAAATT GCAAATGAAT TATGTGAATG CCCAGAGTTA 
CTTGGATCAG CATTTAGGAA AGTAAAATAT AGTGGAAGTA AAACTGACTC ATCCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLC ( NM_001197115.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001184044.1 Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 2, mRNA.

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CloneID	OHu55651
Clone ID Related Accession (Same CDS sequence)	NM_001197115.1 , NM_001197115.2
Accession Version	NM_001197115.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	glutamate--cysteine ligase catalytic subunit isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI550814.1, AL531593.3, AK313480.1, AB516284.1, AB262176.1 and BC039894.1. On May 31, 2019 this sequence version replaced NM_001197115.1. Transcript Variant: This variant (2, also referred to as delta4) lacks an in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform b), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB516284.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AAACCAAGCG CCATGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
GAAAATAAAA AAGTCCGGTT GGTCCTGTCT GGGGAGAAAG TTCTTGAAAC TCTGCAAGAG 
AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTTTGGA GACCAGAGTA TGGGAGTTAC 
ATGATTGAAG GGACACCAGG ACAGCCCTAC GGAGGAACAA TGTCCGAGTT CAATACAGTT 
GAGGCCAACA TGCGAAAACG CCGGAAGGAG GCTACTTCTA TATTAGAAGA AAATCAGGCT 
CTTTGCACAA TAACTTCATT TCCCAGTACC TTAACAAGAA ATATCCGACA TAGGAGAGGA 
GAAAAGGTTG TCATCAATGT ACCAATATTT AAGGACAAGA ATACACCATC TCCATTTATA 
GAAACATTTA CTGAGGATGA TGAAGCTTCA AGGGCTTCTA AGCCGGATCA TATTTACATG 
GATGCCATGG GATTTGGAAT GGGCAATTGC TGTCTCCAGG TGACATTCCA AGCCTGCAGT 
ATATCTGAGG CCAGATACCT TTATGATCAG TTGGCTACTA TCTGTCCAAT TGTTATGGCT 
TTGAGTGCTG CATCTCCCTT TTACCGAGGC TATGTGTCAG ACATTGATTG TCGCTGGGGA 
GTGATTTCTG CATCTGTAGA TGATAGAACT CGGGAGGAGC GAGGACTGGA GCCATTGAAG 
AACAATAACT ATAGGATCAG TAAATCCCGA TATGACTCAA TAGACAGCTA TTTATCTAAG 
TGTGGTGAGA AATATAATGA CATCGACTTG ACGATAGATA AAGAGATCTA CGAACAGCTG 
TTGCAGGAAG GCATTGATCA TCTCCTGGCC CAGCATGTTG CTCATCTCTT TATTAGAGAC 
CCACTGACAC TGTTTGAAGA GAAAATACAC CTGGATGATG CTAATGAGTC TGACCATTTT 
GAGAATATTC AGTCCACAAA TTGGCAGACA ATGAGATTTA AGCCCCCTCC TCCAAACTCA 
GACATTGGAT GGAGAGTAGA ATTTCGACCC ATGGAGGTGC AATTAACAGA CTTTGAGAAC 
TCTGCCTATG TGGTGTTTGT GGTACTGCTC ACCAGAGTGA TCCTTTCCTA CAAATTGGAT 
TTTCTCATTC CACTGTCAAA GGTTGATGAG AACATGAAGG TAGCACAGAA AAGAGATGCT 
GTCTTGCAGG GAATGTTTTA TTTCAGGAAA GATATTTGCA AAGGTGGCAA TGCAGTGGTG 
GATGGTTGTG GCAAGGCCCA GAACAGCACG GAGCTCGCTG CAGAGGAGTA CACCCTCATG 
AGCATAGACA CCATCATCAA TGGGAAGGAA GGTGTGTTTC CTGGACTGAT CCCAATTCTG 
AACTCTTACC TTGAAAACAT GGAAGTGGAT GTGGACACCA GATGTAGTAT TCTGAACTAC 
CTAAAGCTAA TTAAGAAGAG AGCATCTGGA GAACTAATGA CAGTTGCCAG ATGGATGAGG 
GAGTTTATCG CAAACCATCC TGACTACAAG CAAGACAGTG TCATAACTGA TGAAATGAAT 
TATAGCCTTA TTTTGAAGTG TAACCAAATT GCAAATGAAT TATGTGAATG CCCAGAGTTA 
CTTGGATCAG CATTTAGGAA AGTAAAATAT AGTGGAAGTA AAACTGACTC ATCCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001184044.1
CDS	457..2256
Translation	MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNNYRISKSRYDSIDSYLSKCGEKYNDIDLTIDKEIYEQLLQEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQGMFYFRKDICKGGNAVVDGCGKAQNSTELAAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELLGSAFRKVKYSGSKTDSSN

Target ORF information:

RefSeq Version	NM_001197115.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001197115.2

ORF Insert Sequence:

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AAACCAAGCG CCATGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TTCTCAAGTG GGGCGATGAG GTGGAATACA TGTTGGTATC TTTTGATCAT 
GAAAATAAAA AAGTCCGGTT GGTCCTGTCT GGGGAGAAAG TTCTTGAAAC TCTGCAAGAG 
AAGGGGGAAA GGACAAACCC AAACCATCCT ACCCTTTGGA GACCAGAGTA TGGGAGTTAC 
ATGATTGAAG GGACACCAGG ACAGCCCTAC GGAGGAACAA TGTCCGAGTT CAATACAGTT 
GAGGCCAACA TGCGAAAACG CCGGAAGGAG GCTACTTCTA TATTAGAAGA AAATCAGGCT 
CTTTGCACAA TAACTTCATT TCCCAGTACC TTAACAAGAA ATATCCGACA TAGGAGAGGA 
GAAAAGGTTG TCATCAATGT ACCAATATTT AAGGACAAGA ATACACCATC TCCATTTATA 
GAAACATTTA CTGAGGATGA TGAAGCTTCA AGGGCTTCTA AGCCGGATCA TATTTACATG 
GATGCCATGG GATTTGGAAT GGGCAATTGC TGTCTCCAGG TGACATTCCA AGCCTGCAGT 
ATATCTGAGG CCAGATACCT TTATGATCAG TTGGCTACTA TCTGTCCAAT TGTTATGGCT 
TTGAGTGCTG CATCTCCCTT TTACCGAGGC TATGTGTCAG ACATTGATTG TCGCTGGGGA 
GTGATTTCTG CATCTGTAGA TGATAGAACT CGGGAGGAGC GAGGACTGGA GCCATTGAAG 
AACAATAACT ATAGGATCAG TAAATCCCGA TATGACTCAA TAGACAGCTA TTTATCTAAG 
TGTGGTGAGA AATATAATGA CATCGACTTG ACGATAGATA AAGAGATCTA CGAACAGCTG 
TTGCAGGAAG GCATTGATCA TCTCCTGGCC CAGCATGTTG CTCATCTCTT TATTAGAGAC 
CCACTGACAC TGTTTGAAGA GAAAATACAC CTGGATGATG CTAATGAGTC TGACCATTTT 
GAGAATATTC AGTCCACAAA TTGGCAGACA ATGAGATTTA AGCCCCCTCC TCCAAACTCA 
GACATTGGAT GGAGAGTAGA ATTTCGACCC ATGGAGGTGC AATTAACAGA CTTTGAGAAC 
TCTGCCTATG TGGTGTTTGT GGTACTGCTC ACCAGAGTGA TCCTTTCCTA CAAATTGGAT 
TTTCTCATTC CACTGTCAAA GGTTGATGAG AACATGAAGG TAGCACAGAA AAGAGATGCT 
GTCTTGCAGG GAATGTTTTA TTTCAGGAAA GATATTTGCA AAGGTGGCAA TGCAGTGGTG 
GATGGTTGTG GCAAGGCCCA GAACAGCACG GAGCTCGCTG CAGAGGAGTA CACCCTCATG 
AGCATAGACA CCATCATCAA TGGGAAGGAA GGTGTGTTTC CTGGACTGAT CCCAATTCTG 
AACTCTTACC TTGAAAACAT GGAAGTGGAT GTGGACACCA GATGTAGTAT TCTGAACTAC 
CTAAAGCTAA TTAAGAAGAG AGCATCTGGA GAACTAATGA CAGTTGCCAG ATGGATGAGG 
GAGTTTATCG CAAACCATCC TGACTACAAG CAAGACAGTG TCATAACTGA TGAAATGAAT 
TATAGCCTTA TTTTGAAGTG TAACCAAATT GCAAATGAAT TATGTGAATG CCCAGAGTTA 
CTTGGATCAG CATTTAGGAA AGTAAAATAT AGTGGAAGTA AAACTGACTC ATCCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLC ( XM_017010749.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866238.1 Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X1, mRNA.

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CloneID	OHu96543
Clone ID Related Accession (Same CDS sequence)	XM_017010749.1
Accession Version	XM_017010749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	glutamate--cysteine ligase catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCA TGGGATTTGG AATGGGCAAT TGCTGTCTCC AGGTGACATT CCAAGCCTGC 
AGTATATCTG AGGCCAGATA CCTTTATGAT CAGTTGGCTA CTATCTGTCC AATTGTTATG 
GCTTTGAGTG CTGCATCTCC CTTTTACCGA GGCTATGTGT CAGACATTGA TTGTCGCTGG 
GGAGTGATTT CTGCATCTGT AGATGATAGA ACTCGGGAGG AGCGAGGACT GGAGCCATTG 
AAGAACAATA ACTATAGGAT CAGTAAATCC CGATATGACT CAATAGACAG CTATTTATCT 
AAGTGTGGTG AGAAATATAA TGACATCGAC TTGACGATAG ATAAAGAGAT CTACGAACAG 
CTGTTGCAGG AAGGCATTGA TCATCTCCTG GCCCAGCATG TTGCTCATCT CTTTATTAGA 
GACCCACTGA CACTGTTTGA AGAGAAAATA CACCTGGATG ATGCTAATGA GTCTGACCAT 
TTTGAGAATA TTCAGTCCAC AAATTGGCAG ACAATGAGAT TTAAGCCCCC TCCTCCAAAC 
TCAGACATTG GATGGAGAGT AGAATTTCGA CCCATGGAGG TGCAATTAAC AGACTTTGAG 
AACTCTGCCT ATGTGGTGTT TGTGGTACTG CTCACCAGAG TGATCCTTTC CTACAAATTG 
GATTTTCTCA TTCCACTGTC AAAGGTTGAT GAGAACATGA AGGTAGCACA GAAAAGAGAT 
GCTGTCTTGC AGGGAATGTT TTATTTCAGG AAAGATATTT GCAAAGGTGG CAATGCAGTG 
GTGGATGGTT GTGGCAAGGC CCAGAACAGC ACGGAGCTCG CTGCAGAGGA GTACACCCTC 
ATGAGCATAG ACACCATCAT CAATGGGAAG GAAGGTGTGT TTCCTGGACT GATCCCAATT 
CTGAACTCTT ACCTTGAAAA CATGGAAGTG GATGTGGACA CCAGATGTAG TATTCTGAAC 
TACCTAAAGC TAATTAAGAA GAGAGCATCT GGAGAACTAA TGACAGTTGC CAGATGGATG 
AGGGAGTTTA TCGCAAACCA TCCTGACTAC AAGCAAGACA GTGTCATAAC TGATGAAATG 
AATTATAGCC TTATTTTGAA GTGTAACCAA ATTGCAAATG AATTATGTGA ATGCCCAGAG 
TTACTTGGAT CAGCATTTAG GAAAGTAAAA TATAGTGGAA GTAAAACTGA CTCATCCAAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866238.1
CDS	821..2023
Translation	MDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNNYRISKSRYDSIDSYLSKCGEKYNDIDLTIDKEIYEQLLQEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQGMFYFRKDICKGGNAVVDGCGKAQNSTELAAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEMNYSLILKCNQIANELCECPELLGSAFRKVKYSGSKTDSSN

Target ORF information:

RefSeq Version	XM_017010749.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010749.1

ORF Insert Sequence:

ATGGATGCCA TGGGATTTGG AATGGGCAAT TGCTGTCTCC AGGTGACATT CCAAGCCTGC 
AGTATATCTG AGGCCAGATA CCTTTATGAT CAGTTGGCTA CTATCTGTCC AATTGTTATG 
GCTTTGAGTG CTGCATCTCC CTTTTACCGA GGCTATGTGT CAGACATTGA TTGTCGCTGG 
GGAGTGATTT CTGCATCTGT AGATGATAGA ACTCGGGAGG AGCGAGGACT GGAGCCATTG 
AAGAACAATA ACTATAGGAT CAGTAAATCC CGATATGACT CAATAGACAG CTATTTATCT 
AAGTGTGGTG AGAAATATAA TGACATCGAC TTGACGATAG ATAAAGAGAT CTACGAACAG 
CTGTTGCAGG AAGGCATTGA TCATCTCCTG GCCCAGCATG TTGCTCATCT CTTTATTAGA 
GACCCACTGA CACTGTTTGA AGAGAAAATA CACCTGGATG ATGCTAATGA GTCTGACCAT 
TTTGAGAATA TTCAGTCCAC AAATTGGCAG ACAATGAGAT TTAAGCCCCC TCCTCCAAAC 
TCAGACATTG GATGGAGAGT AGAATTTCGA CCCATGGAGG TGCAATTAAC AGACTTTGAG 
AACTCTGCCT ATGTGGTGTT TGTGGTACTG CTCACCAGAG TGATCCTTTC CTACAAATTG 
GATTTTCTCA TTCCACTGTC AAAGGTTGAT GAGAACATGA AGGTAGCACA GAAAAGAGAT 
GCTGTCTTGC AGGGAATGTT TTATTTCAGG AAAGATATTT GCAAAGGTGG CAATGCAGTG 
GTGGATGGTT GTGGCAAGGC CCAGAACAGC ACGGAGCTCG CTGCAGAGGA GTACACCCTC 
ATGAGCATAG ACACCATCAT CAATGGGAAG GAAGGTGTGT TTCCTGGACT GATCCCAATT 
CTGAACTCTT ACCTTGAAAA CATGGAAGTG GATGTGGACA CCAGATGTAG TATTCTGAAC 
TACCTAAAGC TAATTAAGAA GAGAGCATCT GGAGAACTAA TGACAGTTGC CAGATGGATG 
AGGGAGTTTA TCGCAAACCA TCCTGACTAC AAGCAAGACA GTGTCATAAC TGATGAAATG 
AATTATAGCC TTATTTTGAA GTGTAACCAA ATTGCAAATG AATTATGTGA ATGCCCAGAG 
TTACTTGGAT CAGCATTTAG GAAAGTAAAA TATAGTGGAA GTAAAACTGA CTCATCCAAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transformation of liver cells by 3-methylcholanthrene potentiates oxidative stress via the downregulation of glutathione synthesis.
International journal of molecular medicine40(6)2011-2017(2017 Dec)
Kim SH,Yoon D,Lee YH,Lee J,Kim ND,Kim S,Jung YS

Polymorphism of Glutamate-Cysteine Ligase Subunit Catalytic (GCLC) Gene in Pulmonary Tuberculosis Patients.
Pakistan journal of biological sciences : PJBS20(8)397-402(2017)
Yuniastuti A,Susanti R,Mustikaningtyas D

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GCLC cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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