GCLC cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCLC
Entrez Gene ID	512468
Full Name	glutamate-cysteine ligase catalytic subunit
General protein information	Preferred Names glutamate-cysteine ligase catalytic subunit Names glutamate--cysteine ligase catalytic subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024983546.1	XP_024839314.1	glutamate--cysteine ligase catalytic subunit isoform X1
XM_005223236.4	XP_005223293.1	glutamate--cysteine ligase catalytic subunit isoform X3
XM_005223237.4	XP_005223294.1	glutamate--cysteine ligase catalytic subunit isoform X4
XM_024983547.1	XP_024839315.1	glutamate--cysteine ligase catalytic subunit isoform X2
NM_001083674.1	NP_001077143.1	glutamate--cysteine ligase catalytic subunit
XM_005223236.3	XP_005223293.1	glutamate--cysteine ligase catalytic subunit isoform X1
XM_005223237.3	XP_005223294.1	glutamate--cysteine ligase catalytic subunit isoform X2
XM_024983548.1	XP_024839316.1	glutamate--cysteine ligase catalytic subunit isoform X5

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gclc	NP_036947.1
Saccharomyces cerevisiae (baker's yeast)	GSH1	NP_012434.1
Kluyveromyces lactis	KLLA0F14058g	XP_455711.1
Schizosaccharomyces pombe (fission yeast)	gcs1	NP_593031.1
Magnaporthe oryzae (rice blast fungus)	MGG_07317	XP_003715549.1
Pan troglodytes (chimpanzee)	GCLC	XP_001155930.1
	GCLC	XP_001108943.1
Danio rerio (zebrafish)	gclc	NP_954971.2
Drosophila melanogaster (fruit fly)	Gclc	NP_525005.2
Caenorhabditis elegans (roundworm)	gcs-1	NP_495927.2
Canis lupus familiaris (dog)	GCLC	XP_852845.1
Bos taurus (cattle)	GCLC	NP_001077143.1
Mus musculus (house mouse)	Gclc	NP_034425.1
Gallus gallus (chicken)	GCLC	XP_419910.3
Eremothecium gossypii	AGOS_AFR086C	NP_985633.1
Neurospora crassa	NCU01157	XP_961523.1
Xenopus tropicalis (tropical clawed frog)	gclc	NP_001072803.1
Homo sapiens (human)	GCLC	NP_001489.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001512	XP_321610.3

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GCLC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCLC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa39035	NM_001083674.1 Latest version!	Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa31873	XM_005223236.4 Latest version!	Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa31874	XM_005223237.4 Latest version!	Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa229467	XM_024983546.1 Latest version!	Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa229468	XM_024983547.1 Latest version!	Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa229469	XM_024983548.1 Latest version!	Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OBa31873	XM_005223236.3	Bos taurus glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa31874	XM_005223237.3	Bos taurus glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCLC ( NM_001083674.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001077143.1 Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), mRNA.

Price & Availability↑

CloneID	OBa39035
Clone ID Related Accession (Same CDS sequence)	NM_001083674.1
Accession Version	NM_001083674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	glutamate--cysteine ligase catalytic subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY957499.1. On Apr 5, 2007 this sequence version replaced XM_865715.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC 
AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC 
AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA 
GTGGTCATCA ACGTGCCCAC TCCCCACCTG CCCAAGGATC ACCCGGGCCC AGCCCAGGTA 
ACGTTCCAAG CCTGCAGCAT ATCTGAGGCC AGATACCTTT ACGATCAGTT GGCTACCATC 
TGCCCAATTG TCATGGCTTT GAGTGCTGCG TCGCCTTTTT ACCGAGGCTA CGTGTCAGAC 
ATTGATTGTC GCTGGGGCGT GATTTCCGCG TCTGTAGATG ATAGAACTCG GGAGGAGAGA 
GGACTGGAGC CACTGAAGAA CAACCACTAT AGGATCAGTA AGTCCCGATA CGATTCGATA 
GACAGTTACT TGTCTGAGTG TGGTGAGAAA TACAATGACA TTGACCTGAC AAAAGACAAA 
GAAATCTACG AACAGCTGTT GCAGGAAGGC CTCGACCACC TGCTGGCCCA GCACGTGGCG 
CATCTCTTCA TCAGAGACCC GCTGACTCTG TTTGAAGAGA AAATCCACCT GGATGATGCC 
AACGAGTCCG ACCACTTTGA GAATATTCAG TCCACAAATT GGCAGACAAT GCGATTCAAG 
CCTCCTCCTC CAAACTCTGA CATTGGGTGG AGAGTGGAAT TCCGGCCCAT GGAGGTTCAG 
CTGACAGACT TCGAGAACTC CGCGTATGTG GTGTTCGTGG TGCTGCTCAC CAGGGTGATC 
CTGTCCTACA AACTGGACTT TCTCATCCCG CTGTCCAAGG TTGATGAGAA CATGAAGGTC 
GCCCAGAAGC GAGATGCCGT CCTGCAGGAA CTGTTTTATT TCAGGAAAGA CATTTGCAAA 
GGTGGCAACG CCGTGGTGGA CGGCTGCGGC AAGGCCGCGG GCAGGGCGGA GCCAGCGGCG 
GAGGAGTACG CGCTCATGAC CATCGACACC ATCATCAACG GCAAGGAAGG CGTGTTCCCC 
GGGCTGATCC CCGTCCTGAA CTCCTACCTG GAGAGCATGG AGGTGGACGT GGACACCAGG 
TGCAGCATTC TGAACTACCT GAAGCTCATC AAGAAGAGGG CGTCCGGAGA ATTAATGACA 
GTTGCCAAAT GGATGAGGGA GTTTATCGCA AAGCATCCCG ACTACAAGCA GGACAGTGTA 
ATAACTGACC AGATGAACTA TAGCCTTCTT CTAAAGTGTA ATCAAATCGC AAACGAATCG 
TGTGAGTGCC CAGAGTTACT CGGACCAGCG TTTAGGAAAG TCAGGTACAG TGGAAGTAAA 
ACCGACTCTT CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077143.1
CDS	1..1818
Translation	MGLLSQGSPLSWEETQRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHEKKKAQLVLSGEELLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEDNMRKRRKEATSLLGENQALCTITSFPRLGCPGFTLPEFKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPTPHLPKDHPGPAQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNHYRISKSRYDSIDSYLSECGEKYNDIDLTKDKEIYEQLLQEGLDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQELFYFRKDICKGGNAVVDGCGKAAGRAEPAAEEYALMTIDTIINGKEGVFPGLIPVLNSYLESMEVDVDTRCSILNYLKLIKKRASGELMTVAKWMREFIAKHPDYKQDSVITDQMNYSLLLKCNQIANESCECPELLGPAFRKVRYSGSKTDSST

Target ORF information:

RefSeq Version	NM_001083674.1
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083674.1

ORF Insert Sequence:

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC 
AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC 
AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA 
GTGGTCATCA ACGTGCCCAC TCCCCACCTG CCCAAGGATC ACCCGGGCCC AGCCCAGGTA 
ACGTTCCAAG CCTGCAGCAT ATCTGAGGCC AGATACCTTT ACGATCAGTT GGCTACCATC 
TGCCCAATTG TCATGGCTTT GAGTGCTGCG TCGCCTTTTT ACCGAGGCTA CGTGTCAGAC 
ATTGATTGTC GCTGGGGCGT GATTTCCGCG TCTGTAGATG ATAGAACTCG GGAGGAGAGA 
GGACTGGAGC CACTGAAGAA CAACCACTAT AGGATCAGTA AGTCCCGATA CGATTCGATA 
GACAGTTACT TGTCTGAGTG TGGTGAGAAA TACAATGACA TTGACCTGAC AAAAGACAAA 
GAAATCTACG AACAGCTGTT GCAGGAAGGC CTCGACCACC TGCTGGCCCA GCACGTGGCG 
CATCTCTTCA TCAGAGACCC GCTGACTCTG TTTGAAGAGA AAATCCACCT GGATGATGCC 
AACGAGTCCG ACCACTTTGA GAATATTCAG TCCACAAATT GGCAGACAAT GCGATTCAAG 
CCTCCTCCTC CAAACTCTGA CATTGGGTGG AGAGTGGAAT TCCGGCCCAT GGAGGTTCAG 
CTGACAGACT TCGAGAACTC CGCGTATGTG GTGTTCGTGG TGCTGCTCAC CAGGGTGATC 
CTGTCCTACA AACTGGACTT TCTCATCCCG CTGTCCAAGG TTGATGAGAA CATGAAGGTC 
GCCCAGAAGC GAGATGCCGT CCTGCAGGAA CTGTTTTATT TCAGGAAAGA CATTTGCAAA 
GGTGGCAACG CCGTGGTGGA CGGCTGCGGC AAGGCCGCGG GCAGGGCGGA GCCAGCGGCG 
GAGGAGTACG CGCTCATGAC CATCGACACC ATCATCAACG GCAAGGAAGG CGTGTTCCCC 
GGGCTGATCC CCGTCCTGAA CTCCTACCTG GAGAGCATGG AGGTGGACGT GGACACCAGG 
TGCAGCATTC TGAACTACCT GAAGCTCATC AAGAAGAGGG CGTCCGGAGA ATTAATGACA 
GTTGCCAAAT GGATGAGGGA GTTTATCGCA AAGCATCCCG ACTACAAGCA GGACAGTGTA 
ATAACTGACC AGATGAACTA TAGCCTTCTT CTAAAGTGTA ATCAAATCGC AAACGAATCG 
TGTGAGTGCC CAGAGTTACT CGGACCAGCG TTTAGGAAAG TCAGGTACAG TGGAAGTAAA 
ACCGACTCTT CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLC ( XM_005223236.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223293.1 Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa31873
Clone ID Related Accession (Same CDS sequence)	XM_005223236.4 , XM_005223236.3
Accession Version	XM_005223236.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	glutamate--cysteine ligase catalytic subunit isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223236.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC 
AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC 
AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA 
GTGGTCATCA ACGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT 
TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC 
ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT 
GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT 
GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT 
TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC 
CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT 
GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG 
GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG 
ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT 
ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT 
GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG 
TATGTGGTGT TCGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC 
ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG 
CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGCAG GTGGCAACGC CGTGGTGGAC 
GGCTGCGGCA AGGCCGCGGG CAGGGCGGAG CCAGCGGCGG AGGAGTACGC GCTCATGACC 
ATCGACACCA TCATCAACGG CAAGGAAGGC GTGTTCCCCG GGCTGATCCC CGTCCTGAAC 
TCCTACCTGG AGAGCATGGA GGTGGACGTG GACACCAGGT GCAGCATTCT GAACTACCTG 
AAGCTCATCA AGAAGAGGGC GTCCGGAGAA TTAATGACAG TTGCCAAATG GATGAGGGAG 
TTTATCGCAA AGCATCCCGA CTACAAGCAG GACAGTGTAA TAACTGACCA GATGAACTAT 
AGCCTTCTTC TAAAGTGTAA TCAAATCGCA AACGAATCGT GTGAGTGCCC AGAGTTACTC 
GGACCAGCGT TTAGGAAAGT CAGGTACAGT GGAAGTAAAA CCGACTCTTC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223293.1
CDS	29..1945
Translation	MGLLSQGSPLSWEETQRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHEKKKAQLVLSGEELLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEDNMRKRRKEATSLLGENQALCTITSFPRLGCPGFTLPEFKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPAPFIETFPEDEEATKASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNHYRISKSRYDSIDSYLSECGEKYNDIDLTKDKEIYEQLLQEGLDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQELFYFRKDICKAGGNAVVDGCGKAAGRAEPAAEEYALMTIDTIINGKEGVFPGLIPVLNSYLESMEVDVDTRCSILNYLKLIKKRASGELMTVAKWMREFIAKHPDYKQDSVITDQMNYSLLLKCNQIANESCECPELLGPAFRKVRYSGSKTDSST

Target ORF information:

RefSeq Version	XM_005223236.4
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223236.4

ORF Insert Sequence:

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC 
AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC 
AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA 
GTGGTCATCA ACGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT 
TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC 
ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT 
GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT 
GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT 
TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC 
CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT 
GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG 
GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG 
ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT 
ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT 
GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG 
TATGTGGTGT TCGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC 
ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG 
CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGCAG GTGGCAACGC CGTGGTGGAC 
GGCTGCGGCA AGGCCGCGGG CAGGGCGGAG CCAGCGGCGG AGGAGTACGC GCTCATGACC 
ATCGACACCA TCATCAACGG CAAGGAAGGC GTGTTCCCCG GGCTGATCCC CGTCCTGAAC 
TCCTACCTGG AGAGCATGGA GGTGGACGTG GACACCAGGT GCAGCATTCT GAACTACCTG 
AAGCTCATCA AGAAGAGGGC GTCCGGAGAA TTAATGACAG TTGCCAAATG GATGAGGGAG 
TTTATCGCAA AGCATCCCGA CTACAAGCAG GACAGTGTAA TAACTGACCA GATGAACTAT 
AGCCTTCTTC TAAAGTGTAA TCAAATCGCA AACGAATCGT GTGAGTGCCC AGAGTTACTC 
GGACCAGCGT TTAGGAAAGT CAGGTACAGT GGAAGTAAAA CCGACTCTTC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLC ( XM_005223236.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223293.1 Bos taurus glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa31873
Clone ID Related Accession (Same CDS sequence)	XM_005223236.4 , XM_005223236.3
Accession Version	XM_005223236.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	glutamate--cysteine ligase catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104548.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223236.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC 
AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC 
AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA 
GTGGTCATCA ACGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT 
TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC 
ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT 
GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT 
GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT 
TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC 
CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT 
GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG 
GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG 
ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT 
ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT 
GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG 
TATGTGGTGT TCGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC 
ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG 
CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGCAG GTGGCAACGC CGTGGTGGAC 
GGCTGCGGCA AGGCCGCGGG CAGGGCGGAG CCAGCGGCGG AGGAGTACGC GCTCATGACC 
ATCGACACCA TCATCAACGG CAAGGAAGGC GTGTTCCCCG GGCTGATCCC CGTCCTGAAC 
TCCTACCTGG AGAGCATGGA GGTGGACGTG GACACCAGGT GCAGCATTCT GAACTACCTG 
AAGCTCATCA AGAAGAGGGC GTCCGGAGAA TTAATGACAG TTGCCAAATG GATGAGGGAG 
TTTATCGCAA AGCATCCCGA CTACAAGCAG GACAGTGTAA TAACTGACCA GATGAACTAT 
AGCCTTCTTC TAAAGTGTAA TCAAATCGCA AACGAATCGT GTGAGTGCCC AGAGTTACTC 
GGACCAGCGT TTAGGAAAGT CAGGTACAGT GGAAGTAAAA CCGACTCTTC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223293.1
CDS	448..2364
Translation	MGLLSQGSPLSWEETQRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHEKKKAQLVLSGEELLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEDNMRKRRKEATSLLGENQALCTITSFPRLGCPGFTLPEFKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPAPFIETFPEDEEATKASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNHYRISKSRYDSIDSYLSECGEKYNDIDLTKDKEIYEQLLQEGLDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQELFYFRKDICKAGGNAVVDGCGKAAGRAEPAAEEYALMTIDTIINGKEGVFPGLIPVLNSYLESMEVDVDTRCSILNYLKLIKKRASGELMTVAKWMREFIAKHPDYKQDSVITDQMNYSLLLKCNQIANESCECPELLGPAFRKVRYSGSKTDSST

Target ORF information:

RefSeq Version	XM_005223236.3
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223236.3

ORF Insert Sequence:

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC 
AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC 
AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA 
GTGGTCATCA ACGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT 
TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC 
ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT 
GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT 
GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT 
TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC 
CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT 
GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG 
GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG 
ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT 
ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT 
GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG 
TATGTGGTGT TCGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC 
ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG 
CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGCAG GTGGCAACGC CGTGGTGGAC 
GGCTGCGGCA AGGCCGCGGG CAGGGCGGAG CCAGCGGCGG AGGAGTACGC GCTCATGACC 
ATCGACACCA TCATCAACGG CAAGGAAGGC GTGTTCCCCG GGCTGATCCC CGTCCTGAAC 
TCCTACCTGG AGAGCATGGA GGTGGACGTG GACACCAGGT GCAGCATTCT GAACTACCTG 
AAGCTCATCA AGAAGAGGGC GTCCGGAGAA TTAATGACAG TTGCCAAATG GATGAGGGAG 
TTTATCGCAA AGCATCCCGA CTACAAGCAG GACAGTGTAA TAACTGACCA GATGAACTAT 
AGCCTTCTTC TAAAGTGTAA TCAAATCGCA AACGAATCGT GTGAGTGCCC AGAGTTACTC 
GGACCAGCGT TTAGGAAAGT CAGGTACAGT GGAAGTAAAA CCGACTCTTC CACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLC ( XM_005223237.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223294.1 Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa31874
Clone ID Related Accession (Same CDS sequence)	XM_005223237.4 , XM_005223237.3
Accession Version	XM_005223237.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	glutamate--cysteine ligase catalytic subunit isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223237.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC 
AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC 
AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA 
GTGGTCATCA ACGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT 
TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC 
ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT 
GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT 
GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT 
TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC 
CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT 
GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG 
GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG 
ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT 
ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT 
GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG 
TATGTGGTGT TCGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC 
ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG 
CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGGTG GCAACGCCGT GGTGGACGGC 
TGCGGCAAGG CCGCGGGCAG GGCGGAGCCA GCGGCGGAGG AGTACGCGCT CATGACCATC 
GACACCATCA TCAACGGCAA GGAAGGCGTG TTCCCCGGGC TGATCCCCGT CCTGAACTCC 
TACCTGGAGA GCATGGAGGT GGACGTGGAC ACCAGGTGCA GCATTCTGAA CTACCTGAAG 
CTCATCAAGA AGAGGGCGTC CGGAGAATTA ATGACAGTTG CCAAATGGAT GAGGGAGTTT 
ATCGCAAAGC ATCCCGACTA CAAGCAGGAC AGTGTAATAA CTGACCAGAT GAACTATAGC 
CTTCTTCTAA AGTGTAATCA AATCGCAAAC GAATCGTGTG AGTGCCCAGA GTTACTCGGA 
CCAGCGTTTA GGAAAGTCAG GTACAGTGGA AGTAAAACCG ACTCTTCCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223294.1
CDS	1..1914
Translation	MGLLSQGSPLSWEETQRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHEKKKAQLVLSGEELLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEDNMRKRRKEATSLLGENQALCTITSFPRLGCPGFTLPEFKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPAPFIETFPEDEEATKASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNHYRISKSRYDSIDSYLSECGEKYNDIDLTKDKEIYEQLLQEGLDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQELFYFRKDICKGGNAVVDGCGKAAGRAEPAAEEYALMTIDTIINGKEGVFPGLIPVLNSYLESMEVDVDTRCSILNYLKLIKKRASGELMTVAKWMREFIAKHPDYKQDSVITDQMNYSLLLKCNQIANESCECPELLGPAFRKVRYSGSKTDSST

Target ORF information:

RefSeq Version	XM_005223237.4
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223237.4

ORF Insert Sequence:

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC 
AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC 
AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA 
GTGGTCATCA ACGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT 
TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC 
ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT 
GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT 
GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT 
TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC 
CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT 
GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG 
GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG 
ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT 
ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT 
GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG 
TATGTGGTGT TCGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC 
ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG 
CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGGTG GCAACGCCGT GGTGGACGGC 
TGCGGCAAGG CCGCGGGCAG GGCGGAGCCA GCGGCGGAGG AGTACGCGCT CATGACCATC 
GACACCATCA TCAACGGCAA GGAAGGCGTG TTCCCCGGGC TGATCCCCGT CCTGAACTCC 
TACCTGGAGA GCATGGAGGT GGACGTGGAC ACCAGGTGCA GCATTCTGAA CTACCTGAAG 
CTCATCAAGA AGAGGGCGTC CGGAGAATTA ATGACAGTTG CCAAATGGAT GAGGGAGTTT 
ATCGCAAAGC ATCCCGACTA CAAGCAGGAC AGTGTAATAA CTGACCAGAT GAACTATAGC 
CTTCTTCTAA AGTGTAATCA AATCGCAAAC GAATCGTGTG AGTGCCCAGA GTTACTCGGA 
CCAGCGTTTA GGAAAGTCAG GTACAGTGGA AGTAAAACCG ACTCTTCCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLC ( XM_005223237.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005223294.1 Bos taurus glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa31874
Clone ID Related Accession (Same CDS sequence)	XM_005223237.4 , XM_005223237.3
Accession Version	XM_005223237.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	glutamate--cysteine ligase catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104548.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223237.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC 
AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC 
AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA 
GTGGTCATCA ACGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT 
TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC 
ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT 
GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT 
GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT 
TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC 
CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT 
GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG 
GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG 
ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT 
ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT 
GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG 
TATGTGGTGT TCGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC 
ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG 
CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGGTG GCAACGCCGT GGTGGACGGC 
TGCGGCAAGG CCGCGGGCAG GGCGGAGCCA GCGGCGGAGG AGTACGCGCT CATGACCATC 
GACACCATCA TCAACGGCAA GGAAGGCGTG TTCCCCGGGC TGATCCCCGT CCTGAACTCC 
TACCTGGAGA GCATGGAGGT GGACGTGGAC ACCAGGTGCA GCATTCTGAA CTACCTGAAG 
CTCATCAAGA AGAGGGCGTC CGGAGAATTA ATGACAGTTG CCAAATGGAT GAGGGAGTTT 
ATCGCAAAGC ATCCCGACTA CAAGCAGGAC AGTGTAATAA CTGACCAGAT GAACTATAGC 
CTTCTTCTAA AGTGTAATCA AATCGCAAAC GAATCGTGTG AGTGCCCAGA GTTACTCGGA 
CCAGCGTTTA GGAAAGTCAG GTACAGTGGA AGTAAAACCG ACTCTTCCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005223294.1
CDS	448..2361
Translation	MGLLSQGSPLSWEETQRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHEKKKAQLVLSGEELLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEDNMRKRRKEATSLLGENQALCTITSFPRLGCPGFTLPEFKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPAPFIETFPEDEEATKASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNHYRISKSRYDSIDSYLSECGEKYNDIDLTKDKEIYEQLLQEGLDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQELFYFRKDICKGGNAVVDGCGKAAGRAEPAAEEYALMTIDTIINGKEGVFPGLIPVLNSYLESMEVDVDTRCSILNYLKLIKKRASGELMTVAKWMREFIAKHPDYKQDSVITDQMNYSLLLKCNQIANESCECPELLGPAFRKVRYSGSKTDSST

Target ORF information:

RefSeq Version	XM_005223237.3
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005223237.3

ORF Insert Sequence:

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC 
AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC 
AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA 
GTGGTCATCA ACGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT 
TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC 
ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT 
GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT 
GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT 
TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC 
CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT 
GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG 
GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG 
ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT 
ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT 
GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG 
TATGTGGTGT TCGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC 
ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG 
CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGGTG GCAACGCCGT GGTGGACGGC 
TGCGGCAAGG CCGCGGGCAG GGCGGAGCCA GCGGCGGAGG AGTACGCGCT CATGACCATC 
GACACCATCA TCAACGGCAA GGAAGGCGTG TTCCCCGGGC TGATCCCCGT CCTGAACTCC 
TACCTGGAGA GCATGGAGGT GGACGTGGAC ACCAGGTGCA GCATTCTGAA CTACCTGAAG 
CTCATCAAGA AGAGGGCGTC CGGAGAATTA ATGACAGTTG CCAAATGGAT GAGGGAGTTT 
ATCGCAAAGC ATCCCGACTA CAAGCAGGAC AGTGTAATAA CTGACCAGAT GAACTATAGC 
CTTCTTCTAA AGTGTAATCA AATCGCAAAC GAATCGTGTG AGTGCCCAGA GTTACTCGGA 
CCAGCGTTTA GGAAAGTCAG GTACAGTGGA AGTAAAACCG ACTCTTCCAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLC ( XM_024983546.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839314.1 Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa229467
Clone ID Related Accession (Same CDS sequence)	XM_024983546.1
Accession Version	XM_024983546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	glutamate--cysteine ligase catalytic subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGTTCT TCAATTTGTT GTAGATTAGG CTGCCCTGGG 
TTCACGCTGC CCGAGTTCAA ACCCAACCCG GTGGAAGGAG GAGCTTCCAA GTCCCTTTTC 
TTTCCTGATG AAGCCATCAA CAAGCACCCG CGCTTCAGCA CGCTCACCAG GAACATCCGG 
CACCGGAGGG GCGAGAAAGT GGTCATCAAC GTGCCCATCT TTAAGGACAA GAACACCCCA 
GCTCCATTTA TAGAGACTTT CCCTGAGGAC GAGGAGGCCA CGAAGGCCTC TAAGCCCGAC 
CACATTTACA TGGACGCCAT GGGCTTCGGG ATGGGCAACT GCTGCCTACA GGTAACGTTC 
CAAGCCTGCA GCATATCTGA GGCCAGATAC CTTTACGATC AGTTGGCTAC CATCTGCCCA 
ATTGTCATGG CTTTGAGTGC TGCGTCGCCT TTTTACCGAG GCTACGTGTC AGACATTGAT 
TGTCGCTGGG GCGTGATTTC CGCGTCTGTA GATGATAGAA CTCGGGAGGA GAGAGGACTG 
GAGCCACTGA AGAACAACCA CTATAGGATC AGTAAGTCCC GATACGATTC GATAGACAGT 
TACTTGTCTG AGTGTGGTGA GAAATACAAT GACATTGACC TGACAAAAGA CAAAGAAATC 
TACGAACAGC TGTTGCAGGA AGGCCTCGAC CACCTGCTGG CCCAGCACGT GGCGCATCTC 
TTCATCAGAG ACCCGCTGAC TCTGTTTGAA GAGAAAATCC ACCTGGATGA TGCCAACGAG 
TCCGACCACT TTGAGAATAT TCAGTCCACA AATTGGCAGA CAATGCGATT CAAGCCTCCT 
CCTCCAAACT CTGACATTGG GTGGAGAGTG GAATTCCGGC CCATGGAGGT TCAGCTGACA 
GACTTCGAGA ACTCCGCGTA TGTGGTGTTC GTGGTGCTGC TCACCAGGGT GATCCTGTCC 
TACAAACTGG ACTTTCTCAT CCCGCTGTCC AAGGTTGATG AGAACATGAA GGTCGCCCAG 
AAGCGAGATG CCGTCCTGCA GGAACTGTTT TATTTCAGGA AAGACATTTG CAAAGCAGGT 
GGCAACGCCG TGGTGGACGG CTGCGGCAAG GCCGCGGGCA GGGCGGAGCC AGCGGCGGAG 
GAGTACGCGC TCATGACCAT CGACACCATC ATCAACGGCA AGGAAGGCGT GTTCCCCGGG 
CTGATCCCCG TCCTGAACTC CTACCTGGAG AGCATGGAGG TGGACGTGGA CACCAGGTGC 
AGCATTCTGA ACTACCTGAA GCTCATCAAG AAGAGGGCGT CCGGAGAATT AATGACAGTT 
GCCAAATGGA TGAGGGAGTT TATCGCAAAG CATCCCGACT ACAAGCAGGA CAGTGTAATA 
ACTGACCAGA TGAACTATAG CCTTCTTCTA AAGTGTAATC AAATCGCAAA CGAATCGTGT 
GAGTGCCCAG AGTTACTCGG ACCAGCGTTT AGGAAAGTCA GGTACAGTGG AAGTAAAACC 
GACTCTTCCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839314.1
CDS	473..2407
Translation	MGLLSQGSPLSWEETQRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHEKKKAQLVLSGEELLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEDNMRKRRKEATSLLGENQALCTITSFPSSSICCRLGCPGFTLPEFKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPAPFIETFPEDEEATKASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNHYRISKSRYDSIDSYLSECGEKYNDIDLTKDKEIYEQLLQEGLDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQELFYFRKDICKAGGNAVVDGCGKAAGRAEPAAEEYALMTIDTIINGKEGVFPGLIPVLNSYLESMEVDVDTRCSILNYLKLIKKRASGELMTVAKWMREFIAKHPDYKQDSVITDQMNYSLLLKCNQIANESCECPELLGPAFRKVRYSGSKTDSST

Target ORF information:

RefSeq Version	XM_024983546.1
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983546.1

ORF Insert Sequence:

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGTTCT TCAATTTGTT GTAGATTAGG CTGCCCTGGG 
TTCACGCTGC CCGAGTTCAA ACCCAACCCG GTGGAAGGAG GAGCTTCCAA GTCCCTTTTC 
TTTCCTGATG AAGCCATCAA CAAGCACCCG CGCTTCAGCA CGCTCACCAG GAACATCCGG 
CACCGGAGGG GCGAGAAAGT GGTCATCAAC GTGCCCATCT TTAAGGACAA GAACACCCCA 
GCTCCATTTA TAGAGACTTT CCCTGAGGAC GAGGAGGCCA CGAAGGCCTC TAAGCCCGAC 
CACATTTACA TGGACGCCAT GGGCTTCGGG ATGGGCAACT GCTGCCTACA GGTAACGTTC 
CAAGCCTGCA GCATATCTGA GGCCAGATAC CTTTACGATC AGTTGGCTAC CATCTGCCCA 
ATTGTCATGG CTTTGAGTGC TGCGTCGCCT TTTTACCGAG GCTACGTGTC AGACATTGAT 
TGTCGCTGGG GCGTGATTTC CGCGTCTGTA GATGATAGAA CTCGGGAGGA GAGAGGACTG 
GAGCCACTGA AGAACAACCA CTATAGGATC AGTAAGTCCC GATACGATTC GATAGACAGT 
TACTTGTCTG AGTGTGGTGA GAAATACAAT GACATTGACC TGACAAAAGA CAAAGAAATC 
TACGAACAGC TGTTGCAGGA AGGCCTCGAC CACCTGCTGG CCCAGCACGT GGCGCATCTC 
TTCATCAGAG ACCCGCTGAC TCTGTTTGAA GAGAAAATCC ACCTGGATGA TGCCAACGAG 
TCCGACCACT TTGAGAATAT TCAGTCCACA AATTGGCAGA CAATGCGATT CAAGCCTCCT 
CCTCCAAACT CTGACATTGG GTGGAGAGTG GAATTCCGGC CCATGGAGGT TCAGCTGACA 
GACTTCGAGA ACTCCGCGTA TGTGGTGTTC GTGGTGCTGC TCACCAGGGT GATCCTGTCC 
TACAAACTGG ACTTTCTCAT CCCGCTGTCC AAGGTTGATG AGAACATGAA GGTCGCCCAG 
AAGCGAGATG CCGTCCTGCA GGAACTGTTT TATTTCAGGA AAGACATTTG CAAAGCAGGT 
GGCAACGCCG TGGTGGACGG CTGCGGCAAG GCCGCGGGCA GGGCGGAGCC AGCGGCGGAG 
GAGTACGCGC TCATGACCAT CGACACCATC ATCAACGGCA AGGAAGGCGT GTTCCCCGGG 
CTGATCCCCG TCCTGAACTC CTACCTGGAG AGCATGGAGG TGGACGTGGA CACCAGGTGC 
AGCATTCTGA ACTACCTGAA GCTCATCAAG AAGAGGGCGT CCGGAGAATT AATGACAGTT 
GCCAAATGGA TGAGGGAGTT TATCGCAAAG CATCCCGACT ACAAGCAGGA CAGTGTAATA 
ACTGACCAGA TGAACTATAG CCTTCTTCTA AAGTGTAATC AAATCGCAAA CGAATCGTGT 
GAGTGCCCAG AGTTACTCGG ACCAGCGTTT AGGAAAGTCA GGTACAGTGG AAGTAAAACC 
GACTCTTCCA CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLC ( XM_024983547.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839315.1 Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa229468
Clone ID Related Accession (Same CDS sequence)	XM_024983547.1
Accession Version	XM_024983547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	glutamate--cysteine ligase catalytic subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGTTCT TCAATTTGTT GTAGATTAGG CTGCCCTGGG 
TTCACGCTGC CCGAGTTCAA ACCCAACCCG GTGGAAGGAG GAGCTTCCAA GTCCCTTTTC 
TTTCCTGATG AAGCCATCAA CAAGCACCCG CGCTTCAGCA CGCTCACCAG GAACATCCGG 
CACCGGAGGG GCGAGAAAGT GGTCATCAAC GTGCCCATCT TTAAGGACAA GAACACCCCA 
GCTCCATTTA TAGAGACTTT CCCTGAGGAC GAGGAGGCCA CGAAGGCCTC TAAGCCCGAC 
CACATTTACA TGGACGCCAT GGGCTTCGGG ATGGGCAACT GCTGCCTACA GGTAACGTTC 
CAAGCCTGCA GCATATCTGA GGCCAGATAC CTTTACGATC AGTTGGCTAC CATCTGCCCA 
ATTGTCATGG CTTTGAGTGC TGCGTCGCCT TTTTACCGAG GCTACGTGTC AGACATTGAT 
TGTCGCTGGG GCGTGATTTC CGCGTCTGTA GATGATAGAA CTCGGGAGGA GAGAGGACTG 
GAGCCACTGA AGAACAACCA CTATAGGATC AGTAAGTCCC GATACGATTC GATAGACAGT 
TACTTGTCTG AGTGTGGTGA GAAATACAAT GACATTGACC TGACAAAAGA CAAAGAAATC 
TACGAACAGC TGTTGCAGGA AGGCCTCGAC CACCTGCTGG CCCAGCACGT GGCGCATCTC 
TTCATCAGAG ACCCGCTGAC TCTGTTTGAA GAGAAAATCC ACCTGGATGA TGCCAACGAG 
TCCGACCACT TTGAGAATAT TCAGTCCACA AATTGGCAGA CAATGCGATT CAAGCCTCCT 
CCTCCAAACT CTGACATTGG GTGGAGAGTG GAATTCCGGC CCATGGAGGT TCAGCTGACA 
GACTTCGAGA ACTCCGCGTA TGTGGTGTTC GTGGTGCTGC TCACCAGGGT GATCCTGTCC 
TACAAACTGG ACTTTCTCAT CCCGCTGTCC AAGGTTGATG AGAACATGAA GGTCGCCCAG 
AAGCGAGATG CCGTCCTGCA GGAACTGTTT TATTTCAGGA AAGACATTTG CAAAGGTGGC 
AACGCCGTGG TGGACGGCTG CGGCAAGGCC GCGGGCAGGG CGGAGCCAGC GGCGGAGGAG 
TACGCGCTCA TGACCATCGA CACCATCATC AACGGCAAGG AAGGCGTGTT CCCCGGGCTG 
ATCCCCGTCC TGAACTCCTA CCTGGAGAGC ATGGAGGTGG ACGTGGACAC CAGGTGCAGC 
ATTCTGAACT ACCTGAAGCT CATCAAGAAG AGGGCGTCCG GAGAATTAAT GACAGTTGCC 
AAATGGATGA GGGAGTTTAT CGCAAAGCAT CCCGACTACA AGCAGGACAG TGTAATAACT 
GACCAGATGA ACTATAGCCT TCTTCTAAAG TGTAATCAAA TCGCAAACGA ATCGTGTGAG 
TGCCCAGAGT TACTCGGACC AGCGTTTAGG AAAGTCAGGT ACAGTGGAAG TAAAACCGAC 
TCTTCCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839315.1
CDS	473..2404
Translation	MGLLSQGSPLSWEETQRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHEKKKAQLVLSGEELLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEDNMRKRRKEATSLLGENQALCTITSFPSSSICCRLGCPGFTLPEFKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPAPFIETFPEDEEATKASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNHYRISKSRYDSIDSYLSECGEKYNDIDLTKDKEIYEQLLQEGLDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQELFYFRKDICKGGNAVVDGCGKAAGRAEPAAEEYALMTIDTIINGKEGVFPGLIPVLNSYLESMEVDVDTRCSILNYLKLIKKRASGELMTVAKWMREFIAKHPDYKQDSVITDQMNYSLLLKCNQIANESCECPELLGPAFRKVRYSGSKTDSST

Target ORF information:

RefSeq Version	XM_024983547.1
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983547.1

ORF Insert Sequence:

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGTTCT TCAATTTGTT GTAGATTAGG CTGCCCTGGG 
TTCACGCTGC CCGAGTTCAA ACCCAACCCG GTGGAAGGAG GAGCTTCCAA GTCCCTTTTC 
TTTCCTGATG AAGCCATCAA CAAGCACCCG CGCTTCAGCA CGCTCACCAG GAACATCCGG 
CACCGGAGGG GCGAGAAAGT GGTCATCAAC GTGCCCATCT TTAAGGACAA GAACACCCCA 
GCTCCATTTA TAGAGACTTT CCCTGAGGAC GAGGAGGCCA CGAAGGCCTC TAAGCCCGAC 
CACATTTACA TGGACGCCAT GGGCTTCGGG ATGGGCAACT GCTGCCTACA GGTAACGTTC 
CAAGCCTGCA GCATATCTGA GGCCAGATAC CTTTACGATC AGTTGGCTAC CATCTGCCCA 
ATTGTCATGG CTTTGAGTGC TGCGTCGCCT TTTTACCGAG GCTACGTGTC AGACATTGAT 
TGTCGCTGGG GCGTGATTTC CGCGTCTGTA GATGATAGAA CTCGGGAGGA GAGAGGACTG 
GAGCCACTGA AGAACAACCA CTATAGGATC AGTAAGTCCC GATACGATTC GATAGACAGT 
TACTTGTCTG AGTGTGGTGA GAAATACAAT GACATTGACC TGACAAAAGA CAAAGAAATC 
TACGAACAGC TGTTGCAGGA AGGCCTCGAC CACCTGCTGG CCCAGCACGT GGCGCATCTC 
TTCATCAGAG ACCCGCTGAC TCTGTTTGAA GAGAAAATCC ACCTGGATGA TGCCAACGAG 
TCCGACCACT TTGAGAATAT TCAGTCCACA AATTGGCAGA CAATGCGATT CAAGCCTCCT 
CCTCCAAACT CTGACATTGG GTGGAGAGTG GAATTCCGGC CCATGGAGGT TCAGCTGACA 
GACTTCGAGA ACTCCGCGTA TGTGGTGTTC GTGGTGCTGC TCACCAGGGT GATCCTGTCC 
TACAAACTGG ACTTTCTCAT CCCGCTGTCC AAGGTTGATG AGAACATGAA GGTCGCCCAG 
AAGCGAGATG CCGTCCTGCA GGAACTGTTT TATTTCAGGA AAGACATTTG CAAAGGTGGC 
AACGCCGTGG TGGACGGCTG CGGCAAGGCC GCGGGCAGGG CGGAGCCAGC GGCGGAGGAG 
TACGCGCTCA TGACCATCGA CACCATCATC AACGGCAAGG AAGGCGTGTT CCCCGGGCTG 
ATCCCCGTCC TGAACTCCTA CCTGGAGAGC ATGGAGGTGG ACGTGGACAC CAGGTGCAGC 
ATTCTGAACT ACCTGAAGCT CATCAAGAAG AGGGCGTCCG GAGAATTAAT GACAGTTGCC 
AAATGGATGA GGGAGTTTAT CGCAAAGCAT CCCGACTACA AGCAGGACAG TGTAATAACT 
GACCAGATGA ACTATAGCCT TCTTCTAAAG TGTAATCAAA TCGCAAACGA ATCGTGTGAG 
TGCCCAGAGT TACTCGGACC AGCGTTTAGG AAAGTCAGGT ACAGTGGAAG TAAAACCGAC 
TCTTCCACTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCLC ( XM_024983548.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839316.1 Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa229469
Clone ID Related Accession (Same CDS sequence)	XM_024983548.1
Accession Version	XM_024983548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	glutamate--cysteine ligase catalytic subunit isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGTTCT TCAATTTGTT GTAGATTAGG CTGCCCTGGG 
TTCACGCTGC CCGAGTTCAA ACCCAACCCG GTGGAAGGAG GAGCTTCCAA GTCCCTTTTC 
TTTCCTGATG AAGCCATCAA CAAGCACCCG CGCTTCAGCA CGCTCACCAG GAACATCCGG 
CACCGGAGGG GCGAGAAAGT GGTCATCAAC GTGCCCATCT TTAAGGACAA GAACACCCCA 
GCTCCATTTA TAGAGACTTT CCCTGAGGAC GAGGAGGCCA CGAAGGCCTC TAAGCCCGAC 
CACATTTACA TGGACGCCAT GGGCTTCGGG ATGGGCAACT GCTGCCTACA GGTAACGTTC 
CAAGCCTGCA GCATATCTGA GGCCAGATAC CTTTACGATC AGTTGGCTAC CATCTGCCCA 
ATTGTCCCAC TGAAGAACAA CCACTATAGG ATCAGTAAGT CCCGATACGA TTCGATAGAC 
AGTTACTTGT CTGAGTGTGG TGAGAAATAC AATGACATTG ACCTGACAAA AGACAAAGAA 
ATCTACGAAC AGCTGTTGCA GGAAGGCCTC GACCACCTGC TGGCCCAGCA CGTGGCGCAT 
CTCTTCATCA GAGACCCGCT GACTCTGTTT GAAGAGAAAA TCCACCTGGA TGATGCCAAC 
GAGTCCGACC ACTTTGAGAA TATTCAGTCC ACAAATTGGC AGACAATGCG ATTCAAGCCT 
CCTCCTCCAA ACTCTGACAT TGGGTGGAGA GTGGAATTCC GGCCCATGGA GGTTCAGCTG 
ACAGACTTCG AGAACTCCGC GTATGTGGTG TTCGTGGTGC TGCTCACCAG GGTGATCCTG 
TCCTACAAAC TGGACTTTCT CATCCCGCTG TCCAAGGTTG ATGAGAACAT GAAGGTCGCC 
CAGAAGCGAG ATGCCGTCCT GCAGGAACTG TTTTATTTCA GGAAAGACAT TTGCAAAGCA 
GGTGGCAACG CCGTGGTGGA CGGCTGCGGC AAGGCCGCGG GCAGGGCGGA GCCAGCGGCG 
GAGGAGTACG CGCTCATGAC CATCGACACC ATCATCAACG GCAAGGAAGG CGTGTTCCCC 
GGGCTGATCC CCGTCCTGAA CTCCTACCTG GAGAGCATGG AGGTGGACGT GGACACCAGG 
TGCAGCATTC TGAACTACCT GAAGCTCATC AAGAAGAGGG CGTCCGGAGA ATTAATGACA 
GTTGCCAAAT GGATGAGGGA GTTTATCGCA AAGCATCCCG ACTACAAGCA GGACAGTGTA 
ATAACTGACC AGATGAACTA TAGCCTTCTT CTAAAGTGTA ATCAAATCGC AAACGAATCG 
TGTGAGTGCC CAGAGTTACT CGGACCAGCG TTTAGGAAAG TCAGGTACAG TGGAAGTAAA 
ACCGACTCTT CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839316.1
CDS	474..2291
Translation	MGLLSQGSPLSWEETQRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHEKKKAQLVLSGEELLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEDNMRKRRKEATSLLGENQALCTITSFPSSSICCRLGCPGFTLPEFKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPAPFIETFPEDEEATKASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVPLKNNHYRISKSRYDSIDSYLSECGEKYNDIDLTKDKEIYEQLLQEGLDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQELFYFRKDICKAGGNAVVDGCGKAAGRAEPAAEEYALMTIDTIINGKEGVFPGLIPVLNSYLESMEVDVDTRCSILNYLKLIKKRASGELMTVAKWMREFIAKHPDYKQDSVITDQMNYSLLLKCNQIANESCECPELLGPAFRKVRYSGSKTDSST

Target ORF information:

RefSeq Version	XM_024983548.1
Organism	Bos taurus(cattle)
Definition	Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983548.1

ORF Insert Sequence:

ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG 
CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGTTCT TCAATTTGTT GTAGATTAGG CTGCCCTGGG 
TTCACGCTGC CCGAGTTCAA ACCCAACCCG GTGGAAGGAG GAGCTTCCAA GTCCCTTTTC 
TTTCCTGATG AAGCCATCAA CAAGCACCCG CGCTTCAGCA CGCTCACCAG GAACATCCGG 
CACCGGAGGG GCGAGAAAGT GGTCATCAAC GTGCCCATCT TTAAGGACAA GAACACCCCA 
GCTCCATTTA TAGAGACTTT CCCTGAGGAC GAGGAGGCCA CGAAGGCCTC TAAGCCCGAC 
CACATTTACA TGGACGCCAT GGGCTTCGGG ATGGGCAACT GCTGCCTACA GGTAACGTTC 
CAAGCCTGCA GCATATCTGA GGCCAGATAC CTTTACGATC AGTTGGCTAC CATCTGCCCA 
ATTGTCCCAC TGAAGAACAA CCACTATAGG ATCAGTAAGT CCCGATACGA TTCGATAGAC 
AGTTACTTGT CTGAGTGTGG TGAGAAATAC AATGACATTG ACCTGACAAA AGACAAAGAA 
ATCTACGAAC AGCTGTTGCA GGAAGGCCTC GACCACCTGC TGGCCCAGCA CGTGGCGCAT 
CTCTTCATCA GAGACCCGCT GACTCTGTTT GAAGAGAAAA TCCACCTGGA TGATGCCAAC 
GAGTCCGACC ACTTTGAGAA TATTCAGTCC ACAAATTGGC AGACAATGCG ATTCAAGCCT 
CCTCCTCCAA ACTCTGACAT TGGGTGGAGA GTGGAATTCC GGCCCATGGA GGTTCAGCTG 
ACAGACTTCG AGAACTCCGC GTATGTGGTG TTCGTGGTGC TGCTCACCAG GGTGATCCTG 
TCCTACAAAC TGGACTTTCT CATCCCGCTG TCCAAGGTTG ATGAGAACAT GAAGGTCGCC 
CAGAAGCGAG ATGCCGTCCT GCAGGAACTG TTTTATTTCA GGAAAGACAT TTGCAAAGCA 
GGTGGCAACG CCGTGGTGGA CGGCTGCGGC AAGGCCGCGG GCAGGGCGGA GCCAGCGGCG 
GAGGAGTACG CGCTCATGAC CATCGACACC ATCATCAACG GCAAGGAAGG CGTGTTCCCC 
GGGCTGATCC CCGTCCTGAA CTCCTACCTG GAGAGCATGG AGGTGGACGT GGACACCAGG 
TGCAGCATTC TGAACTACCT GAAGCTCATC AAGAAGAGGG CGTCCGGAGA ATTAATGACA 
GTTGCCAAAT GGATGAGGGA GTTTATCGCA AAGCATCCCG ACTACAAGCA GGACAGTGTA 
ATAACTGACC AGATGAACTA TAGCCTTCTT CTAAAGTGTA ATCAAATCGC AAACGAATCG 
TGTGAGTGCC CAGAGTTACT CGGACCAGCG TTTAGGAAAG TCAGGTACAG TGGAAGTAAA 
ACCGACTCTT CCACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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GCLC cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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