Gclc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gclc
Entrez Gene ID	53581
Full Name	Glutamate-cysteine ligase catalytic subunit
Synonyms	2259,CG2259,DmGCLC,DmGCS,DmGCSh,Dmel\CG2259,GCL,GCLC,GCLc,GCSh,GclC,Gcs,Gcsh,gclc
General protein information	Preferred Names Glutamate-cysteine ligase catalytic subunit Names CG2259 gene product from transcript CG2259-RC CG2259-PA CG2259-PB CG2259-PC CG2259-PD Gclc-PA Gclc-PB Gclc-PC Gclc-PD catalytic subunit of glutamate- cysteine ligase gamma-Glutamylcysteine synthetase gamma-Glutamylcysteine synthetase catalytic subunit gamma-glutamylcysteine ligase glutamate cysteine ligase glutamate-cysteine ligase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7D6-7D6

mRNA and Protein(s)

mRNA	Protein	Name
NM_080266.3	NP_525005.2	Glutamate-cysteine ligase catalytic subunit, isoform A
NM_206650.2	NP_996373.1	Glutamate-cysteine ligase catalytic subunit, isoform B
NM_001298072.1	NP_001285001.1	Glutamate-cysteine ligase catalytic subunit, isoform C
NM_001298073.1	NP_001285002.1	Glutamate-cysteine ligase catalytic subunit, isoform D

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gclc	NP_036947.1
Saccharomyces cerevisiae (baker's yeast)	GSH1	NP_012434.1
Kluyveromyces lactis	KLLA0F14058g	XP_455711.1
Schizosaccharomyces pombe (fission yeast)	gcs1	NP_593031.1
Magnaporthe oryzae (rice blast fungus)	MGG_07317	XP_003715549.1
Pan troglodytes (chimpanzee)	GCLC	XP_001155930.1
	GCLC	XP_001108943.1
Danio rerio (zebrafish)	gclc	NP_954971.2
Drosophila melanogaster (fruit fly)	Gclc	NP_525005.2
Caenorhabditis elegans (roundworm)	gcs-1	NP_495927.2
Canis lupus familiaris (dog)	GCLC	XP_852845.1
Bos taurus (cattle)	GCLC	NP_001077143.1
Mus musculus (house mouse)	Gclc	NP_034425.1
Gallus gallus (chicken)	GCLC	XP_419910.3
Eremothecium gossypii	AGOS_AFR086C	NP_985633.1
Neurospora crassa	NCU01157	XP_961523.1
Xenopus tropicalis (tropical clawed frog)	gclc	NP_001072803.1
Homo sapiens (human)	GCLC	NP_001489.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001512	XP_321610.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Microarray analysis revealed that glutamate cysteine ligasec overexpression and thus enhanced glutathione production has a broad impact on gene expression that largely affects different processes in young and old flies.
Title: Age-dependent changes in the transcription profile of long-lived Drosophila over-expressing glutamate cysteine ligase.

GSH biosynthesis in the nucleus is associated with migration of only the GCLc subunit from the cytoplasm into the nucleus, and this migration requires the presence of an intact nuclear localization signal
Title: The catalytic subunit of Drosophila glutamate-cysteine ligase is a nucleocytoplasmic shuttling protein.

that the longevity effects of GCLc are dependent on dosage and that there are specific tissues (mushroom bodies, motor neurons, and transverse muscle cells) particularly sensitive to the benefits of GCLc overexpression.
Title: Modulating longevity in Drosophila by over- and underexpression of glutamate-cysteine ligase.

The reversibility of the dephosphorylation-dependent activation was indicated by the time-dependent inactivation of the in vitro activated Drosophila GCL, by preincubation with MgATP.
Title: Mechanisms of gamma-glutamylcysteine ligase regulation.

Neuronal overexpression of GCLc in a long-lived background extended mean and maximum life spans up to 50%, without affecting the rate of oxygen consumption by the flies
Title: Overexpression of glutamate-cysteine ligase extends life span in Drosophila melanogaster.

The following Gclc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gclc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02468	NM_206650.2 Latest version!	Drosophila melanogaster Glutamate-cysteine ligase catalytic subunit (Gclc), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa02468	NM_001298072.1 Latest version!	Drosophila melanogaster Glutamate-cysteine ligase catalytic subunit (Gclc), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa02468	NM_080266.3 Latest version!	Drosophila melanogaster Glutamate-cysteine ligase catalytic subunit (Gclc), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa02468	NM_001298073.1 Latest version!	Drosophila melanogaster Glutamate-cysteine ligase catalytic subunit (Gclc), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gclc ( NM_080266.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525005.2 Drosophila melanogaster Glutamate-cysteine ligase catalytic subunit (Gclc), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02468
Clone ID Related Accession (Same CDS sequence)	NM_080266.3 , NM_206650.2 , NM_001298072.1 , NM_001298073.1
Accession Version	NM_080266.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Glutamate-cysteine ligase catalytic subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080266.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTCTAC TGAGCGAGGG CAGTCCACTC TCCTGGGAGG AGACCAAGGC GCTGGCTGAT 
CATGTGCGTG AGCATGGCGT CAATCAGTTC ATCAACTTGT ACCACAGACT CAAGGATCGC 
CAGGGTGACA TATTGAAATG GGGCGACGAG GTGGAGTACA TTATTGTCAA GTTCGACGAT 
GAACAGAAGG TGGCACGTGT GGCGCTGCGC GCCCAGGATC TGCTGGCACA GCTCAATGAG 
AAGGAACTGG CCGATCCCAA TGGTGTCAAG TCGCTGTGGC GTCCGGAATA CGGAGCCTAC 
ATGATTGAGG GCACACCGGG CAAACCCTTT GGCGGTCTGA TGGCACACTT CAATCTGGTT 
GAGGCCAATA TGCGCTATCG TCGCGAAGAG GTCACCGAGC TGCTGGCCAA GGACGAGTGC 
GTTATGTCCA TTACGAATTT CCCACGACTC GGCGCACCAA ATTTCACTTA TCCGCTTGCC 
CAGCCGCGTC CCGAGGATCC TCTCAGTTCA GCCCGCTCGC TCTACTTCCC GGATGAGGCC 
ATCTTTCCGG GTCACCCGCG TTTCAAGACA CTCACGCGTA ACATTCGCAA GCGACGCGGC 
GAGAAGGTGT CCATCAAGCT AAAAGTTTTC AAGGACACAA AGACGAAGCT ACCGGTGGAG 
GGTGCTCCGC CTGGCGAACC GGATGTGGTG CTCCTGGACG CCATGGGCTT CGGCATGGGC 
TGCTGTTGCT TGCAGCTCAC CTTCCAGGCC TGTAACATTA CGGAGGCGCG TCGCCTCTAC 
GATCAGCTGG CTCCCCTCTG TCCGATCATG TTGGCTCTGA CGGCCGCTTC GCCCATTTAC 
AGGGGCTATC TAACCGAATC GGATTGTCGA TGGAATGTGA TTAGCTCCTC GGTGGATTGC 
CGCACGGAAG AGGAACGCGG ACTGGCGCCG CTGGATCAGC AAAAGTTCAG GATAGCCAAG 
TCGCGATACG ATTCGATTGA CTCGTATTTG TCGCCCGAGG GAGCCAAATA TAACGATGTA 
CCGCTCACAT ACGATGAGAA GGTCTATCAG CGTCTGGTGG AGGGCGGTAT CGATCATCTG 
TTGGCCCAGC ATGTTGCCCA TTTGTTTATA CGCGACACCG TGTCGCTGTT CAGCGAGAAG 
GTGCATCAAA ACGACAACGA GGATACGGAT CATTTCGAGA ACATTCAGTC CACCAATTGG 
CAGACCATGC GCTTCAAGCC GCCGCCACCG AACAGCTCCA TTGGCTGGCG TGTCGAGTTT 
CGACCTTGCG AGGCGCAGAT CAGTGATTTC GAGAATGCGG CCATCGTGTG CTTTGTGGTG 
CTGCTCACCC GTGTGATTCT CTCCTACCAG CTGAACTTCC TAACGCCGAT CAGCAAGGTG 
GACGAGAACA TGCAGACGGC CCAGAAGCGA GATGCTTGTC GCAAGGAGAA GTTTTGGTTC 
CGCAAATCCT CGAAGACCAC CGAGCAGAGG GCGGCCAAGG CGCAGGCACA AGCTCAAGCG 
CAAGCTAAAG CCCAGGCCCA GACGAATGGC AAGGCCACTT TGAATGGCAA TGGACTGGCG 
AATGGAAATG GGAATGGCAG CGAGAACAGT GACCAGGAAG AGCAGCAGCC TCTGACCAAC 
GGTTCGGCCA AGATGAATGG CCACGGAAGT GGCACCACTA ACGGCACTAA TGGCTCCTCC 
AATGGATCAT CGAATGGCAC CGATAGCGAT CACACCGACA CCGATGACGA GGAGAATGAG 
CTGTTCCAGC TGCTGTCCAT CAATGAGATC TTCAATGGAA AGCCCAACGT TTTTCCGGGT 
CTGGTGCCAT TGATCCGCAG CTATTTGCAG TCCATGGAGG TGGACACCGA TACGCATTGC 
ACCATTGAAC AGTATCTTCG CTTTATCCAG AAGCGTGCCG CCGGCGAGCT AATCACCACG 
GCCACCTGGA TGCGCGAACA GGTGCTTAGC CACCCGGATT ACAAGCAGGA TTCCGTGGTG 
AGCGAGCGCA TCAACTACGA TTTGCTGAAG CGCATCCAAG GCATCCAGGA GGGCAAGCAG 
GTGGAGCCAG CGCTGCTCGG ACAAGACTAT CATTCCAAGA CGAAGACGAA AGACTTCATT 
CCGCCGGCCT TGCAGAAGCA GTTAGCCAAG AACGGCTGCT GCGAGGAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525005.2
CDS	323..2476
Translation	MGLLSEGSPLSWEETKALADHVREHGVNQFINLYHRLKDRQGDILKWGDEVEYIIVKFDDEQKVARVALRAQDLLAQLNEKELADPNGVKSLWRPEYGAYMIEGTPGKPFGGLMAHFNLVEANMRYRREEVTELLAKDECVMSITNFPRLGAPNFTYPLAQPRPEDPLSSARSLYFPDEAIFPGHPRFKTLTRNIRKRRGEKVSIKLKVFKDTKTKLPVEGAPPGEPDVVLLDAMGFGMGCCCLQLTFQACNITEARRLYDQLAPLCPIMLALTAASPIYRGYLTESDCRWNVISSSVDCRTEEERGLAPLDQQKFRIAKSRYDSIDSYLSPEGAKYNDVPLTYDEKVYQRLVEGGIDHLLAQHVAHLFIRDTVSLFSEKVHQNDNEDTDHFENIQSTNWQTMRFKPPPPNSSIGWRVEFRPCEAQISDFENAAIVCFVVLLTRVILSYQLNFLTPISKVDENMQTAQKRDACRKEKFWFRKSSKTTEQRAAKAQAQAQAQAKAQAQTNGKATLNGNGLANGNGNGSENSDQEEQQPLTNGSAKMNGHGSGTTNGTNGSSNGSSNGTDSDHTDTDDEENELFQLLSINEIFNGKPNVFPGLVPLIRSYLQSMEVDTDTHCTIEQYLRFIQKRAAGELITTATWMREQVLSHPDYKQDSVVSERINYDLLKRIQGIQEGKQVEPALLGQDYHSKTKTKDFIPPALQKQLAKNGCCEEK

Target ORF information:

RefSeq Version	NM_080266.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Glutamate-cysteine ligase catalytic subunit (Gclc), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080266.3

ORF Insert Sequence:

ATGGGTCTAC TGAGCGAGGG CAGTCCACTC TCCTGGGAGG AGACCAAGGC GCTGGCTGAT 
CATGTGCGTG AGCATGGCGT CAATCAGTTC ATCAACTTGT ACCACAGACT CAAGGATCGC 
CAGGGTGACA TATTGAAATG GGGCGACGAG GTGGAGTACA TTATTGTCAA GTTCGACGAT 
GAACAGAAGG TGGCACGTGT GGCGCTGCGC GCCCAGGATC TGCTGGCACA GCTCAATGAG 
AAGGAACTGG CCGATCCCAA TGGTGTCAAG TCGCTGTGGC GTCCGGAATA CGGAGCCTAC 
ATGATTGAGG GCACACCGGG CAAACCCTTT GGCGGTCTGA TGGCACACTT CAATCTGGTT 
GAGGCCAATA TGCGCTATCG TCGCGAAGAG GTCACCGAGC TGCTGGCCAA GGACGAGTGC 
GTTATGTCCA TTACGAATTT CCCACGACTC GGCGCACCAA ATTTCACTTA TCCGCTTGCC 
CAGCCGCGTC CCGAGGATCC TCTCAGTTCA GCCCGCTCGC TCTACTTCCC GGATGAGGCC 
ATCTTTCCGG GTCACCCGCG TTTCAAGACA CTCACGCGTA ACATTCGCAA GCGACGCGGC 
GAGAAGGTGT CCATCAAGCT AAAAGTTTTC AAGGACACAA AGACGAAGCT ACCGGTGGAG 
GGTGCTCCGC CTGGCGAACC GGATGTGGTG CTCCTGGACG CCATGGGCTT CGGCATGGGC 
TGCTGTTGCT TGCAGCTCAC CTTCCAGGCC TGTAACATTA CGGAGGCGCG TCGCCTCTAC 
GATCAGCTGG CTCCCCTCTG TCCGATCATG TTGGCTCTGA CGGCCGCTTC GCCCATTTAC 
AGGGGCTATC TAACCGAATC GGATTGTCGA TGGAATGTGA TTAGCTCCTC GGTGGATTGC 
CGCACGGAAG AGGAACGCGG ACTGGCGCCG CTGGATCAGC AAAAGTTCAG GATAGCCAAG 
TCGCGATACG ATTCGATTGA CTCGTATTTG TCGCCCGAGG GAGCCAAATA TAACGATGTA 
CCGCTCACAT ACGATGAGAA GGTCTATCAG CGTCTGGTGG AGGGCGGTAT CGATCATCTG 
TTGGCCCAGC ATGTTGCCCA TTTGTTTATA CGCGACACCG TGTCGCTGTT CAGCGAGAAG 
GTGCATCAAA ACGACAACGA GGATACGGAT CATTTCGAGA ACATTCAGTC CACCAATTGG 
CAGACCATGC GCTTCAAGCC GCCGCCACCG AACAGCTCCA TTGGCTGGCG TGTCGAGTTT 
CGACCTTGCG AGGCGCAGAT CAGTGATTTC GAGAATGCGG CCATCGTGTG CTTTGTGGTG 
CTGCTCACCC GTGTGATTCT CTCCTACCAG CTGAACTTCC TAACGCCGAT CAGCAAGGTG 
GACGAGAACA TGCAGACGGC CCAGAAGCGA GATGCTTGTC GCAAGGAGAA GTTTTGGTTC 
CGCAAATCCT CGAAGACCAC CGAGCAGAGG GCGGCCAAGG CGCAGGCACA AGCTCAAGCG 
CAAGCTAAAG CCCAGGCCCA GACGAATGGC AAGGCCACTT TGAATGGCAA TGGACTGGCG 
AATGGAAATG GGAATGGCAG CGAGAACAGT GACCAGGAAG AGCAGCAGCC TCTGACCAAC 
GGTTCGGCCA AGATGAATGG CCACGGAAGT GGCACCACTA ACGGCACTAA TGGCTCCTCC 
AATGGATCAT CGAATGGCAC CGATAGCGAT CACACCGACA CCGATGACGA GGAGAATGAG 
CTGTTCCAGC TGCTGTCCAT CAATGAGATC TTCAATGGAA AGCCCAACGT TTTTCCGGGT 
CTGGTGCCAT TGATCCGCAG CTATTTGCAG TCCATGGAGG TGGACACCGA TACGCATTGC 
ACCATTGAAC AGTATCTTCG CTTTATCCAG AAGCGTGCCG CCGGCGAGCT AATCACCACG 
GCCACCTGGA TGCGCGAACA GGTGCTTAGC CACCCGGATT ACAAGCAGGA TTCCGTGGTG 
AGCGAGCGCA TCAACTACGA TTTGCTGAAG CGCATCCAAG GCATCCAGGA GGGCAAGCAG 
GTGGAGCCAG CGCTGCTCGG ACAAGACTAT CATTCCAAGA CGAAGACGAA AGACTTCATT 
CCGCCGGCCT TGCAGAAGCA GTTAGCCAAG AACGGCTGCT GCGAGGAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gclc ( NM_206650.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996373.1 Drosophila melanogaster Glutamate-cysteine ligase catalytic subunit (Gclc), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa02468
Clone ID Related Accession (Same CDS sequence)	NM_080266.3 , NM_206650.2 , NM_001298072.1 , NM_001298073.1
Accession Version	NM_206650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Glutamate-cysteine ligase catalytic subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206650.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTCTAC TGAGCGAGGG CAGTCCACTC TCCTGGGAGG AGACCAAGGC GCTGGCTGAT 
CATGTGCGTG AGCATGGCGT CAATCAGTTC ATCAACTTGT ACCACAGACT CAAGGATCGC 
CAGGGTGACA TATTGAAATG GGGCGACGAG GTGGAGTACA TTATTGTCAA GTTCGACGAT 
GAACAGAAGG TGGCACGTGT GGCGCTGCGC GCCCAGGATC TGCTGGCACA GCTCAATGAG 
AAGGAACTGG CCGATCCCAA TGGTGTCAAG TCGCTGTGGC GTCCGGAATA CGGAGCCTAC 
ATGATTGAGG GCACACCGGG CAAACCCTTT GGCGGTCTGA TGGCACACTT CAATCTGGTT 
GAGGCCAATA TGCGCTATCG TCGCGAAGAG GTCACCGAGC TGCTGGCCAA GGACGAGTGC 
GTTATGTCCA TTACGAATTT CCCACGACTC GGCGCACCAA ATTTCACTTA TCCGCTTGCC 
CAGCCGCGTC CCGAGGATCC TCTCAGTTCA GCCCGCTCGC TCTACTTCCC GGATGAGGCC 
ATCTTTCCGG GTCACCCGCG TTTCAAGACA CTCACGCGTA ACATTCGCAA GCGACGCGGC 
GAGAAGGTGT CCATCAAGCT AAAAGTTTTC AAGGACACAA AGACGAAGCT ACCGGTGGAG 
GGTGCTCCGC CTGGCGAACC GGATGTGGTG CTCCTGGACG CCATGGGCTT CGGCATGGGC 
TGCTGTTGCT TGCAGCTCAC CTTCCAGGCC TGTAACATTA CGGAGGCGCG TCGCCTCTAC 
GATCAGCTGG CTCCCCTCTG TCCGATCATG TTGGCTCTGA CGGCCGCTTC GCCCATTTAC 
AGGGGCTATC TAACCGAATC GGATTGTCGA TGGAATGTGA TTAGCTCCTC GGTGGATTGC 
CGCACGGAAG AGGAACGCGG ACTGGCGCCG CTGGATCAGC AAAAGTTCAG GATAGCCAAG 
TCGCGATACG ATTCGATTGA CTCGTATTTG TCGCCCGAGG GAGCCAAATA TAACGATGTA 
CCGCTCACAT ACGATGAGAA GGTCTATCAG CGTCTGGTGG AGGGCGGTAT CGATCATCTG 
TTGGCCCAGC ATGTTGCCCA TTTGTTTATA CGCGACACCG TGTCGCTGTT CAGCGAGAAG 
GTGCATCAAA ACGACAACGA GGATACGGAT CATTTCGAGA ACATTCAGTC CACCAATTGG 
CAGACCATGC GCTTCAAGCC GCCGCCACCG AACAGCTCCA TTGGCTGGCG TGTCGAGTTT 
CGACCTTGCG AGGCGCAGAT CAGTGATTTC GAGAATGCGG CCATCGTGTG CTTTGTGGTG 
CTGCTCACCC GTGTGATTCT CTCCTACCAG CTGAACTTCC TAACGCCGAT CAGCAAGGTG 
GACGAGAACA TGCAGACGGC CCAGAAGCGA GATGCTTGTC GCAAGGAGAA GTTTTGGTTC 
CGCAAATCCT CGAAGACCAC CGAGCAGAGG GCGGCCAAGG CGCAGGCACA AGCTCAAGCG 
CAAGCTAAAG CCCAGGCCCA GACGAATGGC AAGGCCACTT TGAATGGCAA TGGACTGGCG 
AATGGAAATG GGAATGGCAG CGAGAACAGT GACCAGGAAG AGCAGCAGCC TCTGACCAAC 
GGTTCGGCCA AGATGAATGG CCACGGAAGT GGCACCACTA ACGGCACTAA TGGCTCCTCC 
AATGGATCAT CGAATGGCAC CGATAGCGAT CACACCGACA CCGATGACGA GGAGAATGAG 
CTGTTCCAGC TGCTGTCCAT CAATGAGATC TTCAATGGAA AGCCCAACGT TTTTCCGGGT 
CTGGTGCCAT TGATCCGCAG CTATTTGCAG TCCATGGAGG TGGACACCGA TACGCATTGC 
ACCATTGAAC AGTATCTTCG CTTTATCCAG AAGCGTGCCG CCGGCGAGCT AATCACCACG 
GCCACCTGGA TGCGCGAACA GGTGCTTAGC CACCCGGATT ACAAGCAGGA TTCCGTGGTG 
AGCGAGCGCA TCAACTACGA TTTGCTGAAG CGCATCCAAG GCATCCAGGA GGGCAAGCAG 
GTGGAGCCAG CGCTGCTCGG ACAAGACTAT CATTCCAAGA CGAAGACGAA AGACTTCATT 
CCGCCGGCCT TGCAGAAGCA GTTAGCCAAG AACGGCTGCT GCGAGGAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996373.1
CDS	42..2195
Translation	MGLLSEGSPLSWEETKALADHVREHGVNQFINLYHRLKDRQGDILKWGDEVEYIIVKFDDEQKVARVALRAQDLLAQLNEKELADPNGVKSLWRPEYGAYMIEGTPGKPFGGLMAHFNLVEANMRYRREEVTELLAKDECVMSITNFPRLGAPNFTYPLAQPRPEDPLSSARSLYFPDEAIFPGHPRFKTLTRNIRKRRGEKVSIKLKVFKDTKTKLPVEGAPPGEPDVVLLDAMGFGMGCCCLQLTFQACNITEARRLYDQLAPLCPIMLALTAASPIYRGYLTESDCRWNVISSSVDCRTEEERGLAPLDQQKFRIAKSRYDSIDSYLSPEGAKYNDVPLTYDEKVYQRLVEGGIDHLLAQHVAHLFIRDTVSLFSEKVHQNDNEDTDHFENIQSTNWQTMRFKPPPPNSSIGWRVEFRPCEAQISDFENAAIVCFVVLLTRVILSYQLNFLTPISKVDENMQTAQKRDACRKEKFWFRKSSKTTEQRAAKAQAQAQAQAKAQAQTNGKATLNGNGLANGNGNGSENSDQEEQQPLTNGSAKMNGHGSGTTNGTNGSSNGSSNGTDSDHTDTDDEENELFQLLSINEIFNGKPNVFPGLVPLIRSYLQSMEVDTDTHCTIEQYLRFIQKRAAGELITTATWMREQVLSHPDYKQDSVVSERINYDLLKRIQGIQEGKQVEPALLGQDYHSKTKTKDFIPPALQKQLAKNGCCEEK

Target ORF information:

RefSeq Version	NM_206650.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Glutamate-cysteine ligase catalytic subunit (Gclc), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206650.2

ORF Insert Sequence:

ATGGGTCTAC TGAGCGAGGG CAGTCCACTC TCCTGGGAGG AGACCAAGGC GCTGGCTGAT 
CATGTGCGTG AGCATGGCGT CAATCAGTTC ATCAACTTGT ACCACAGACT CAAGGATCGC 
CAGGGTGACA TATTGAAATG GGGCGACGAG GTGGAGTACA TTATTGTCAA GTTCGACGAT 
GAACAGAAGG TGGCACGTGT GGCGCTGCGC GCCCAGGATC TGCTGGCACA GCTCAATGAG 
AAGGAACTGG CCGATCCCAA TGGTGTCAAG TCGCTGTGGC GTCCGGAATA CGGAGCCTAC 
ATGATTGAGG GCACACCGGG CAAACCCTTT GGCGGTCTGA TGGCACACTT CAATCTGGTT 
GAGGCCAATA TGCGCTATCG TCGCGAAGAG GTCACCGAGC TGCTGGCCAA GGACGAGTGC 
GTTATGTCCA TTACGAATTT CCCACGACTC GGCGCACCAA ATTTCACTTA TCCGCTTGCC 
CAGCCGCGTC CCGAGGATCC TCTCAGTTCA GCCCGCTCGC TCTACTTCCC GGATGAGGCC 
ATCTTTCCGG GTCACCCGCG TTTCAAGACA CTCACGCGTA ACATTCGCAA GCGACGCGGC 
GAGAAGGTGT CCATCAAGCT AAAAGTTTTC AAGGACACAA AGACGAAGCT ACCGGTGGAG 
GGTGCTCCGC CTGGCGAACC GGATGTGGTG CTCCTGGACG CCATGGGCTT CGGCATGGGC 
TGCTGTTGCT TGCAGCTCAC CTTCCAGGCC TGTAACATTA CGGAGGCGCG TCGCCTCTAC 
GATCAGCTGG CTCCCCTCTG TCCGATCATG TTGGCTCTGA CGGCCGCTTC GCCCATTTAC 
AGGGGCTATC TAACCGAATC GGATTGTCGA TGGAATGTGA TTAGCTCCTC GGTGGATTGC 
CGCACGGAAG AGGAACGCGG ACTGGCGCCG CTGGATCAGC AAAAGTTCAG GATAGCCAAG 
TCGCGATACG ATTCGATTGA CTCGTATTTG TCGCCCGAGG GAGCCAAATA TAACGATGTA 
CCGCTCACAT ACGATGAGAA GGTCTATCAG CGTCTGGTGG AGGGCGGTAT CGATCATCTG 
TTGGCCCAGC ATGTTGCCCA TTTGTTTATA CGCGACACCG TGTCGCTGTT CAGCGAGAAG 
GTGCATCAAA ACGACAACGA GGATACGGAT CATTTCGAGA ACATTCAGTC CACCAATTGG 
CAGACCATGC GCTTCAAGCC GCCGCCACCG AACAGCTCCA TTGGCTGGCG TGTCGAGTTT 
CGACCTTGCG AGGCGCAGAT CAGTGATTTC GAGAATGCGG CCATCGTGTG CTTTGTGGTG 
CTGCTCACCC GTGTGATTCT CTCCTACCAG CTGAACTTCC TAACGCCGAT CAGCAAGGTG 
GACGAGAACA TGCAGACGGC CCAGAAGCGA GATGCTTGTC GCAAGGAGAA GTTTTGGTTC 
CGCAAATCCT CGAAGACCAC CGAGCAGAGG GCGGCCAAGG CGCAGGCACA AGCTCAAGCG 
CAAGCTAAAG CCCAGGCCCA GACGAATGGC AAGGCCACTT TGAATGGCAA TGGACTGGCG 
AATGGAAATG GGAATGGCAG CGAGAACAGT GACCAGGAAG AGCAGCAGCC TCTGACCAAC 
GGTTCGGCCA AGATGAATGG CCACGGAAGT GGCACCACTA ACGGCACTAA TGGCTCCTCC 
AATGGATCAT CGAATGGCAC CGATAGCGAT CACACCGACA CCGATGACGA GGAGAATGAG 
CTGTTCCAGC TGCTGTCCAT CAATGAGATC TTCAATGGAA AGCCCAACGT TTTTCCGGGT 
CTGGTGCCAT TGATCCGCAG CTATTTGCAG TCCATGGAGG TGGACACCGA TACGCATTGC 
ACCATTGAAC AGTATCTTCG CTTTATCCAG AAGCGTGCCG CCGGCGAGCT AATCACCACG 
GCCACCTGGA TGCGCGAACA GGTGCTTAGC CACCCGGATT ACAAGCAGGA TTCCGTGGTG 
AGCGAGCGCA TCAACTACGA TTTGCTGAAG CGCATCCAAG GCATCCAGGA GGGCAAGCAG 
GTGGAGCCAG CGCTGCTCGG ACAAGACTAT CATTCCAAGA CGAAGACGAA AGACTTCATT 
CCGCCGGCCT TGCAGAAGCA GTTAGCCAAG AACGGCTGCT GCGAGGAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gclc ( NM_001298072.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285001.1 Drosophila melanogaster Glutamate-cysteine ligase catalytic subunit (Gclc), transcript variant C, mRNA.

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CloneID	OFa02468
Clone ID Related Accession (Same CDS sequence)	NM_080266.3 , NM_206650.2 , NM_001298072.1 , NM_001298073.1
Accession Version	NM_001298072.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Glutamate-cysteine ligase catalytic subunit, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTCTAC TGAGCGAGGG CAGTCCACTC TCCTGGGAGG AGACCAAGGC GCTGGCTGAT 
CATGTGCGTG AGCATGGCGT CAATCAGTTC ATCAACTTGT ACCACAGACT CAAGGATCGC 
CAGGGTGACA TATTGAAATG GGGCGACGAG GTGGAGTACA TTATTGTCAA GTTCGACGAT 
GAACAGAAGG TGGCACGTGT GGCGCTGCGC GCCCAGGATC TGCTGGCACA GCTCAATGAG 
AAGGAACTGG CCGATCCCAA TGGTGTCAAG TCGCTGTGGC GTCCGGAATA CGGAGCCTAC 
ATGATTGAGG GCACACCGGG CAAACCCTTT GGCGGTCTGA TGGCACACTT CAATCTGGTT 
GAGGCCAATA TGCGCTATCG TCGCGAAGAG GTCACCGAGC TGCTGGCCAA GGACGAGTGC 
GTTATGTCCA TTACGAATTT CCCACGACTC GGCGCACCAA ATTTCACTTA TCCGCTTGCC 
CAGCCGCGTC CCGAGGATCC TCTCAGTTCA GCCCGCTCGC TCTACTTCCC GGATGAGGCC 
ATCTTTCCGG GTCACCCGCG TTTCAAGACA CTCACGCGTA ACATTCGCAA GCGACGCGGC 
GAGAAGGTGT CCATCAAGCT AAAAGTTTTC AAGGACACAA AGACGAAGCT ACCGGTGGAG 
GGTGCTCCGC CTGGCGAACC GGATGTGGTG CTCCTGGACG CCATGGGCTT CGGCATGGGC 
TGCTGTTGCT TGCAGCTCAC CTTCCAGGCC TGTAACATTA CGGAGGCGCG TCGCCTCTAC 
GATCAGCTGG CTCCCCTCTG TCCGATCATG TTGGCTCTGA CGGCCGCTTC GCCCATTTAC 
AGGGGCTATC TAACCGAATC GGATTGTCGA TGGAATGTGA TTAGCTCCTC GGTGGATTGC 
CGCACGGAAG AGGAACGCGG ACTGGCGCCG CTGGATCAGC AAAAGTTCAG GATAGCCAAG 
TCGCGATACG ATTCGATTGA CTCGTATTTG TCGCCCGAGG GAGCCAAATA TAACGATGTA 
CCGCTCACAT ACGATGAGAA GGTCTATCAG CGTCTGGTGG AGGGCGGTAT CGATCATCTG 
TTGGCCCAGC ATGTTGCCCA TTTGTTTATA CGCGACACCG TGTCGCTGTT CAGCGAGAAG 
GTGCATCAAA ACGACAACGA GGATACGGAT CATTTCGAGA ACATTCAGTC CACCAATTGG 
CAGACCATGC GCTTCAAGCC GCCGCCACCG AACAGCTCCA TTGGCTGGCG TGTCGAGTTT 
CGACCTTGCG AGGCGCAGAT CAGTGATTTC GAGAATGCGG CCATCGTGTG CTTTGTGGTG 
CTGCTCACCC GTGTGATTCT CTCCTACCAG CTGAACTTCC TAACGCCGAT CAGCAAGGTG 
GACGAGAACA TGCAGACGGC CCAGAAGCGA GATGCTTGTC GCAAGGAGAA GTTTTGGTTC 
CGCAAATCCT CGAAGACCAC CGAGCAGAGG GCGGCCAAGG CGCAGGCACA AGCTCAAGCG 
CAAGCTAAAG CCCAGGCCCA GACGAATGGC AAGGCCACTT TGAATGGCAA TGGACTGGCG 
AATGGAAATG GGAATGGCAG CGAGAACAGT GACCAGGAAG AGCAGCAGCC TCTGACCAAC 
GGTTCGGCCA AGATGAATGG CCACGGAAGT GGCACCACTA ACGGCACTAA TGGCTCCTCC 
AATGGATCAT CGAATGGCAC CGATAGCGAT CACACCGACA CCGATGACGA GGAGAATGAG 
CTGTTCCAGC TGCTGTCCAT CAATGAGATC TTCAATGGAA AGCCCAACGT TTTTCCGGGT 
CTGGTGCCAT TGATCCGCAG CTATTTGCAG TCCATGGAGG TGGACACCGA TACGCATTGC 
ACCATTGAAC AGTATCTTCG CTTTATCCAG AAGCGTGCCG CCGGCGAGCT AATCACCACG 
GCCACCTGGA TGCGCGAACA GGTGCTTAGC CACCCGGATT ACAAGCAGGA TTCCGTGGTG 
AGCGAGCGCA TCAACTACGA TTTGCTGAAG CGCATCCAAG GCATCCAGGA GGGCAAGCAG 
GTGGAGCCAG CGCTGCTCGG ACAAGACTAT CATTCCAAGA CGAAGACGAA AGACTTCATT 
CCGCCGGCCT TGCAGAAGCA GTTAGCCAAG AACGGCTGCT GCGAGGAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285001.1
CDS	734..2887
Translation	MGLLSEGSPLSWEETKALADHVREHGVNQFINLYHRLKDRQGDILKWGDEVEYIIVKFDDEQKVARVALRAQDLLAQLNEKELADPNGVKSLWRPEYGAYMIEGTPGKPFGGLMAHFNLVEANMRYRREEVTELLAKDECVMSITNFPRLGAPNFTYPLAQPRPEDPLSSARSLYFPDEAIFPGHPRFKTLTRNIRKRRGEKVSIKLKVFKDTKTKLPVEGAPPGEPDVVLLDAMGFGMGCCCLQLTFQACNITEARRLYDQLAPLCPIMLALTAASPIYRGYLTESDCRWNVISSSVDCRTEEERGLAPLDQQKFRIAKSRYDSIDSYLSPEGAKYNDVPLTYDEKVYQRLVEGGIDHLLAQHVAHLFIRDTVSLFSEKVHQNDNEDTDHFENIQSTNWQTMRFKPPPPNSSIGWRVEFRPCEAQISDFENAAIVCFVVLLTRVILSYQLNFLTPISKVDENMQTAQKRDACRKEKFWFRKSSKTTEQRAAKAQAQAQAQAKAQAQTNGKATLNGNGLANGNGNGSENSDQEEQQPLTNGSAKMNGHGSGTTNGTNGSSNGSSNGTDSDHTDTDDEENELFQLLSINEIFNGKPNVFPGLVPLIRSYLQSMEVDTDTHCTIEQYLRFIQKRAAGELITTATWMREQVLSHPDYKQDSVVSERINYDLLKRIQGIQEGKQVEPALLGQDYHSKTKTKDFIPPALQKQLAKNGCCEEK

Target ORF information:

RefSeq Version	NM_001298072.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Glutamate-cysteine ligase catalytic subunit (Gclc), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298072.1

ORF Insert Sequence:

ATGGGTCTAC TGAGCGAGGG CAGTCCACTC TCCTGGGAGG AGACCAAGGC GCTGGCTGAT 
CATGTGCGTG AGCATGGCGT CAATCAGTTC ATCAACTTGT ACCACAGACT CAAGGATCGC 
CAGGGTGACA TATTGAAATG GGGCGACGAG GTGGAGTACA TTATTGTCAA GTTCGACGAT 
GAACAGAAGG TGGCACGTGT GGCGCTGCGC GCCCAGGATC TGCTGGCACA GCTCAATGAG 
AAGGAACTGG CCGATCCCAA TGGTGTCAAG TCGCTGTGGC GTCCGGAATA CGGAGCCTAC 
ATGATTGAGG GCACACCGGG CAAACCCTTT GGCGGTCTGA TGGCACACTT CAATCTGGTT 
GAGGCCAATA TGCGCTATCG TCGCGAAGAG GTCACCGAGC TGCTGGCCAA GGACGAGTGC 
GTTATGTCCA TTACGAATTT CCCACGACTC GGCGCACCAA ATTTCACTTA TCCGCTTGCC 
CAGCCGCGTC CCGAGGATCC TCTCAGTTCA GCCCGCTCGC TCTACTTCCC GGATGAGGCC 
ATCTTTCCGG GTCACCCGCG TTTCAAGACA CTCACGCGTA ACATTCGCAA GCGACGCGGC 
GAGAAGGTGT CCATCAAGCT AAAAGTTTTC AAGGACACAA AGACGAAGCT ACCGGTGGAG 
GGTGCTCCGC CTGGCGAACC GGATGTGGTG CTCCTGGACG CCATGGGCTT CGGCATGGGC 
TGCTGTTGCT TGCAGCTCAC CTTCCAGGCC TGTAACATTA CGGAGGCGCG TCGCCTCTAC 
GATCAGCTGG CTCCCCTCTG TCCGATCATG TTGGCTCTGA CGGCCGCTTC GCCCATTTAC 
AGGGGCTATC TAACCGAATC GGATTGTCGA TGGAATGTGA TTAGCTCCTC GGTGGATTGC 
CGCACGGAAG AGGAACGCGG ACTGGCGCCG CTGGATCAGC AAAAGTTCAG GATAGCCAAG 
TCGCGATACG ATTCGATTGA CTCGTATTTG TCGCCCGAGG GAGCCAAATA TAACGATGTA 
CCGCTCACAT ACGATGAGAA GGTCTATCAG CGTCTGGTGG AGGGCGGTAT CGATCATCTG 
TTGGCCCAGC ATGTTGCCCA TTTGTTTATA CGCGACACCG TGTCGCTGTT CAGCGAGAAG 
GTGCATCAAA ACGACAACGA GGATACGGAT CATTTCGAGA ACATTCAGTC CACCAATTGG 
CAGACCATGC GCTTCAAGCC GCCGCCACCG AACAGCTCCA TTGGCTGGCG TGTCGAGTTT 
CGACCTTGCG AGGCGCAGAT CAGTGATTTC GAGAATGCGG CCATCGTGTG CTTTGTGGTG 
CTGCTCACCC GTGTGATTCT CTCCTACCAG CTGAACTTCC TAACGCCGAT CAGCAAGGTG 
GACGAGAACA TGCAGACGGC CCAGAAGCGA GATGCTTGTC GCAAGGAGAA GTTTTGGTTC 
CGCAAATCCT CGAAGACCAC CGAGCAGAGG GCGGCCAAGG CGCAGGCACA AGCTCAAGCG 
CAAGCTAAAG CCCAGGCCCA GACGAATGGC AAGGCCACTT TGAATGGCAA TGGACTGGCG 
AATGGAAATG GGAATGGCAG CGAGAACAGT GACCAGGAAG AGCAGCAGCC TCTGACCAAC 
GGTTCGGCCA AGATGAATGG CCACGGAAGT GGCACCACTA ACGGCACTAA TGGCTCCTCC 
AATGGATCAT CGAATGGCAC CGATAGCGAT CACACCGACA CCGATGACGA GGAGAATGAG 
CTGTTCCAGC TGCTGTCCAT CAATGAGATC TTCAATGGAA AGCCCAACGT TTTTCCGGGT 
CTGGTGCCAT TGATCCGCAG CTATTTGCAG TCCATGGAGG TGGACACCGA TACGCATTGC 
ACCATTGAAC AGTATCTTCG CTTTATCCAG AAGCGTGCCG CCGGCGAGCT AATCACCACG 
GCCACCTGGA TGCGCGAACA GGTGCTTAGC CACCCGGATT ACAAGCAGGA TTCCGTGGTG 
AGCGAGCGCA TCAACTACGA TTTGCTGAAG CGCATCCAAG GCATCCAGGA GGGCAAGCAG 
GTGGAGCCAG CGCTGCTCGG ACAAGACTAT CATTCCAAGA CGAAGACGAA AGACTTCATT 
CCGCCGGCCT TGCAGAAGCA GTTAGCCAAG AACGGCTGCT GCGAGGAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gclc ( NM_001298073.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285002.1 Drosophila melanogaster Glutamate-cysteine ligase catalytic subunit (Gclc), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa02468
Clone ID Related Accession (Same CDS sequence)	NM_080266.3 , NM_206650.2 , NM_001298072.1 , NM_001298073.1
Accession Version	NM_001298073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Glutamate-cysteine ligase catalytic subunit, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTCTAC TGAGCGAGGG CAGTCCACTC TCCTGGGAGG AGACCAAGGC GCTGGCTGAT 
CATGTGCGTG AGCATGGCGT CAATCAGTTC ATCAACTTGT ACCACAGACT CAAGGATCGC 
CAGGGTGACA TATTGAAATG GGGCGACGAG GTGGAGTACA TTATTGTCAA GTTCGACGAT 
GAACAGAAGG TGGCACGTGT GGCGCTGCGC GCCCAGGATC TGCTGGCACA GCTCAATGAG 
AAGGAACTGG CCGATCCCAA TGGTGTCAAG TCGCTGTGGC GTCCGGAATA CGGAGCCTAC 
ATGATTGAGG GCACACCGGG CAAACCCTTT GGCGGTCTGA TGGCACACTT CAATCTGGTT 
GAGGCCAATA TGCGCTATCG TCGCGAAGAG GTCACCGAGC TGCTGGCCAA GGACGAGTGC 
GTTATGTCCA TTACGAATTT CCCACGACTC GGCGCACCAA ATTTCACTTA TCCGCTTGCC 
CAGCCGCGTC CCGAGGATCC TCTCAGTTCA GCCCGCTCGC TCTACTTCCC GGATGAGGCC 
ATCTTTCCGG GTCACCCGCG TTTCAAGACA CTCACGCGTA ACATTCGCAA GCGACGCGGC 
GAGAAGGTGT CCATCAAGCT AAAAGTTTTC AAGGACACAA AGACGAAGCT ACCGGTGGAG 
GGTGCTCCGC CTGGCGAACC GGATGTGGTG CTCCTGGACG CCATGGGCTT CGGCATGGGC 
TGCTGTTGCT TGCAGCTCAC CTTCCAGGCC TGTAACATTA CGGAGGCGCG TCGCCTCTAC 
GATCAGCTGG CTCCCCTCTG TCCGATCATG TTGGCTCTGA CGGCCGCTTC GCCCATTTAC 
AGGGGCTATC TAACCGAATC GGATTGTCGA TGGAATGTGA TTAGCTCCTC GGTGGATTGC 
CGCACGGAAG AGGAACGCGG ACTGGCGCCG CTGGATCAGC AAAAGTTCAG GATAGCCAAG 
TCGCGATACG ATTCGATTGA CTCGTATTTG TCGCCCGAGG GAGCCAAATA TAACGATGTA 
CCGCTCACAT ACGATGAGAA GGTCTATCAG CGTCTGGTGG AGGGCGGTAT CGATCATCTG 
TTGGCCCAGC ATGTTGCCCA TTTGTTTATA CGCGACACCG TGTCGCTGTT CAGCGAGAAG 
GTGCATCAAA ACGACAACGA GGATACGGAT CATTTCGAGA ACATTCAGTC CACCAATTGG 
CAGACCATGC GCTTCAAGCC GCCGCCACCG AACAGCTCCA TTGGCTGGCG TGTCGAGTTT 
CGACCTTGCG AGGCGCAGAT CAGTGATTTC GAGAATGCGG CCATCGTGTG CTTTGTGGTG 
CTGCTCACCC GTGTGATTCT CTCCTACCAG CTGAACTTCC TAACGCCGAT CAGCAAGGTG 
GACGAGAACA TGCAGACGGC CCAGAAGCGA GATGCTTGTC GCAAGGAGAA GTTTTGGTTC 
CGCAAATCCT CGAAGACCAC CGAGCAGAGG GCGGCCAAGG CGCAGGCACA AGCTCAAGCG 
CAAGCTAAAG CCCAGGCCCA GACGAATGGC AAGGCCACTT TGAATGGCAA TGGACTGGCG 
AATGGAAATG GGAATGGCAG CGAGAACAGT GACCAGGAAG AGCAGCAGCC TCTGACCAAC 
GGTTCGGCCA AGATGAATGG CCACGGAAGT GGCACCACTA ACGGCACTAA TGGCTCCTCC 
AATGGATCAT CGAATGGCAC CGATAGCGAT CACACCGACA CCGATGACGA GGAGAATGAG 
CTGTTCCAGC TGCTGTCCAT CAATGAGATC TTCAATGGAA AGCCCAACGT TTTTCCGGGT 
CTGGTGCCAT TGATCCGCAG CTATTTGCAG TCCATGGAGG TGGACACCGA TACGCATTGC 
ACCATTGAAC AGTATCTTCG CTTTATCCAG AAGCGTGCCG CCGGCGAGCT AATCACCACG 
GCCACCTGGA TGCGCGAACA GGTGCTTAGC CACCCGGATT ACAAGCAGGA TTCCGTGGTG 
AGCGAGCGCA TCAACTACGA TTTGCTGAAG CGCATCCAAG GCATCCAGGA GGGCAAGCAG 
GTGGAGCCAG CGCTGCTCGG ACAAGACTAT CATTCCAAGA CGAAGACGAA AGACTTCATT 
CCGCCGGCCT TGCAGAAGCA GTTAGCCAAG AACGGCTGCT GCGAGGAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285002.1
CDS	128..2281
Translation	MGLLSEGSPLSWEETKALADHVREHGVNQFINLYHRLKDRQGDILKWGDEVEYIIVKFDDEQKVARVALRAQDLLAQLNEKELADPNGVKSLWRPEYGAYMIEGTPGKPFGGLMAHFNLVEANMRYRREEVTELLAKDECVMSITNFPRLGAPNFTYPLAQPRPEDPLSSARSLYFPDEAIFPGHPRFKTLTRNIRKRRGEKVSIKLKVFKDTKTKLPVEGAPPGEPDVVLLDAMGFGMGCCCLQLTFQACNITEARRLYDQLAPLCPIMLALTAASPIYRGYLTESDCRWNVISSSVDCRTEEERGLAPLDQQKFRIAKSRYDSIDSYLSPEGAKYNDVPLTYDEKVYQRLVEGGIDHLLAQHVAHLFIRDTVSLFSEKVHQNDNEDTDHFENIQSTNWQTMRFKPPPPNSSIGWRVEFRPCEAQISDFENAAIVCFVVLLTRVILSYQLNFLTPISKVDENMQTAQKRDACRKEKFWFRKSSKTTEQRAAKAQAQAQAQAKAQAQTNGKATLNGNGLANGNGNGSENSDQEEQQPLTNGSAKMNGHGSGTTNGTNGSSNGSSNGTDSDHTDTDDEENELFQLLSINEIFNGKPNVFPGLVPLIRSYLQSMEVDTDTHCTIEQYLRFIQKRAAGELITTATWMREQVLSHPDYKQDSVVSERINYDLLKRIQGIQEGKQVEPALLGQDYHSKTKTKDFIPPALQKQLAKNGCCEEK

Target ORF information:

RefSeq Version	NM_001298073.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Glutamate-cysteine ligase catalytic subunit (Gclc), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298073.1

ORF Insert Sequence:

ATGGGTCTAC TGAGCGAGGG CAGTCCACTC TCCTGGGAGG AGACCAAGGC GCTGGCTGAT 
CATGTGCGTG AGCATGGCGT CAATCAGTTC ATCAACTTGT ACCACAGACT CAAGGATCGC 
CAGGGTGACA TATTGAAATG GGGCGACGAG GTGGAGTACA TTATTGTCAA GTTCGACGAT 
GAACAGAAGG TGGCACGTGT GGCGCTGCGC GCCCAGGATC TGCTGGCACA GCTCAATGAG 
AAGGAACTGG CCGATCCCAA TGGTGTCAAG TCGCTGTGGC GTCCGGAATA CGGAGCCTAC 
ATGATTGAGG GCACACCGGG CAAACCCTTT GGCGGTCTGA TGGCACACTT CAATCTGGTT 
GAGGCCAATA TGCGCTATCG TCGCGAAGAG GTCACCGAGC TGCTGGCCAA GGACGAGTGC 
GTTATGTCCA TTACGAATTT CCCACGACTC GGCGCACCAA ATTTCACTTA TCCGCTTGCC 
CAGCCGCGTC CCGAGGATCC TCTCAGTTCA GCCCGCTCGC TCTACTTCCC GGATGAGGCC 
ATCTTTCCGG GTCACCCGCG TTTCAAGACA CTCACGCGTA ACATTCGCAA GCGACGCGGC 
GAGAAGGTGT CCATCAAGCT AAAAGTTTTC AAGGACACAA AGACGAAGCT ACCGGTGGAG 
GGTGCTCCGC CTGGCGAACC GGATGTGGTG CTCCTGGACG CCATGGGCTT CGGCATGGGC 
TGCTGTTGCT TGCAGCTCAC CTTCCAGGCC TGTAACATTA CGGAGGCGCG TCGCCTCTAC 
GATCAGCTGG CTCCCCTCTG TCCGATCATG TTGGCTCTGA CGGCCGCTTC GCCCATTTAC 
AGGGGCTATC TAACCGAATC GGATTGTCGA TGGAATGTGA TTAGCTCCTC GGTGGATTGC 
CGCACGGAAG AGGAACGCGG ACTGGCGCCG CTGGATCAGC AAAAGTTCAG GATAGCCAAG 
TCGCGATACG ATTCGATTGA CTCGTATTTG TCGCCCGAGG GAGCCAAATA TAACGATGTA 
CCGCTCACAT ACGATGAGAA GGTCTATCAG CGTCTGGTGG AGGGCGGTAT CGATCATCTG 
TTGGCCCAGC ATGTTGCCCA TTTGTTTATA CGCGACACCG TGTCGCTGTT CAGCGAGAAG 
GTGCATCAAA ACGACAACGA GGATACGGAT CATTTCGAGA ACATTCAGTC CACCAATTGG 
CAGACCATGC GCTTCAAGCC GCCGCCACCG AACAGCTCCA TTGGCTGGCG TGTCGAGTTT 
CGACCTTGCG AGGCGCAGAT CAGTGATTTC GAGAATGCGG CCATCGTGTG CTTTGTGGTG 
CTGCTCACCC GTGTGATTCT CTCCTACCAG CTGAACTTCC TAACGCCGAT CAGCAAGGTG 
GACGAGAACA TGCAGACGGC CCAGAAGCGA GATGCTTGTC GCAAGGAGAA GTTTTGGTTC 
CGCAAATCCT CGAAGACCAC CGAGCAGAGG GCGGCCAAGG CGCAGGCACA AGCTCAAGCG 
CAAGCTAAAG CCCAGGCCCA GACGAATGGC AAGGCCACTT TGAATGGCAA TGGACTGGCG 
AATGGAAATG GGAATGGCAG CGAGAACAGT GACCAGGAAG AGCAGCAGCC TCTGACCAAC 
GGTTCGGCCA AGATGAATGG CCACGGAAGT GGCACCACTA ACGGCACTAA TGGCTCCTCC 
AATGGATCAT CGAATGGCAC CGATAGCGAT CACACCGACA CCGATGACGA GGAGAATGAG 
CTGTTCCAGC TGCTGTCCAT CAATGAGATC TTCAATGGAA AGCCCAACGT TTTTCCGGGT 
CTGGTGCCAT TGATCCGCAG CTATTTGCAG TCCATGGAGG TGGACACCGA TACGCATTGC 
ACCATTGAAC AGTATCTTCG CTTTATCCAG AAGCGTGCCG CCGGCGAGCT AATCACCACG 
GCCACCTGGA TGCGCGAACA GGTGCTTAGC CACCCGGATT ACAAGCAGGA TTCCGTGGTG 
AGCGAGCGCA TCAACTACGA TTTGCTGAAG CGCATCCAAG GCATCCAGGA GGGCAAGCAG 
GTGGAGCCAG CGCTGCTCGG ACAAGACTAT CATTCCAAGA CGAAGACGAA AGACTTCATT 
CCGCCGGCCT TGCAGAAGCA GTTAGCCAAG AACGGCTGCT GCGAGGAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gclc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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