EEF2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EEF2
Entrez Gene ID	1938
Full Name	eukaryotic translation elongation factor 2
Synonyms	EEF-2,EF-2,EF2,SCA26
General protein information	Preferred Names eukaryotic translation elongation factor 2 Names elongation factor 2 polypeptidyl-tRNA translocase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.3
Summary	This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008].
Disorder MIM:	130610
Disorder Html:	?Spinocerebellar ataxia 26, 609306 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001961.4	NP_001952.1	elongation factor 2
NM_001961.3	NP_001952.1	elongation factor 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC100614367	XP_003316060.2
	EEF2	XP_001118006.2
Drosophila melanogaster (fruit fly)	Ef2b	NP_525105.2
Mus musculus (house mouse)	Eef2	NP_031933.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009441	XP_307854.2
Danio rerio (zebrafish)	eef2b	NP_956752.2
Arabidopsis thaliana (thale cress)	LOS1	NP_849818.1
Oryza sativa (rice)	Os01g0742200	NP_001044211.1
Bos taurus (cattle)	EEF2	NP_001068589.1
Gallus gallus (chicken)	EEF2	NP_990699.1
Caenorhabditis elegans (roundworm)	eef-2	NP_001251010.1
Homo sapiens (human)	EEF2	NP_001952.1
Canis lupus familiaris (dog)	EEF2	XP_533949.3
Rattus norvegicus (Norway rat)	Eef2	NP_058941.1
Xenopus tropicalis (tropical clawed frog)	eef2.1	NP_989255.1

Gene Ontology
Bibliography

Related articles in PubMed

A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling.
Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson J, Babu M
Cell systems5(6)564-577.e12(2017 Dec)

Eukaryotic Elongation Factor 2 (eEF2) is a Potential Biomarker of Prostate Cancer.
Zhang X, Hu L, Du M, Wei X, Zhang J, Hui Y, Chen C, Li G, Hou J
Pathology oncology research : POR(2017 Sep)

Evidence for a Negative Cooperativity between eIF5A and eEF2 on Binding to the Ribosome.
Rossi D, Barbosa NM, Galv?o FC, Boldrin PE, Hershey JW, Zanelli CF, Fraser CS, Valentini SR
PloS one11(4)e0154205(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that binding of eEF2 to the ribosome alters its conformation, resulting in a weakened affinity of eIF5A and impairment of this interplay compromises cell growth due to translation elongation defects.
Title: Evidence for a Negative Cooperativity between eIF5A and eEF2 on Binding to the Ribosome.

Results indicated that the eEF2 gene is overexpressed in the majority of several types of cancers and plays an oncogenic role in cancer cell growth.
Title: The translation elongation factor eEF2 is a novel tumor?associated antigen overexpressed in various types of cancers.

Data indicate that the accumulation of the cleaved C-terminal small fragment of eukaryotic elongation factor 2 (eEF2) in the nucleus, and C-terminal Src kinase (Csk) could enhance the proteolytic cleavage of eEF2.
Title: C-terminal Src kinase (Csk)-mediated phosphorylation of eukaryotic elongation factor 2 (eEF2) promotes proteolytic cleavage and nuclear translocation of eEF2.

The expression levels of three putative HA-regulated proteins (TALDO, ANXA1 and EF2) in control, H2O2-, HA- and HA/H2O2-treated OA chondrocytes were verified by Western blotting and the results indeed support the notion that HA acts in anti-oxidation
Title: Proteomic analysis of osteoarthritic chondrocyte reveals the hyaluronic acid-regulated proteins involved in chondroprotective effect under oxidative stress.

High serum eukaryotic elongation factor 2 level is associated with non-small cell lung cancer.
Title: Clinical value of eukaryotic elongation factor 2 (eEF2) in non-small cell lung cancer patients.

The following EEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25759	NM_001961.4 Latest version!	Homo sapiens eukaryotic translation elongation factor 2 (EEF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
View Old Accession Versions >>
OHu25759	NM_001961.3	Homo sapiens eukaryotic translation elongation factor 2 (EEF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEF2 ( NM_001961.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001952.1 Homo sapiens eukaryotic translation elongation factor 2 (EEF2), mRNA.

Price & Availability↑

CloneID	OHu25759
Clone ID Related Accession (Same CDS sequence)	NM_001961.4 , NM_001961.3
Accession Version	NM_001961.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	elongation factor 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AU118535.1, AY942181.1 and Z11692.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001961.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1087050.1, SRR3476690.957920.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000309311.7/ ENSP00000307940.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGAACT TCACGGTAGA CCAGATCCGC GCCATCATGG ACAAGAAGGC CAACATCCGC 
AACATGTCTG TCATCGCCCA CGTGGACCAT GGCAAGTCCA CGCTGACAGA CTCCCTGGTG 
TGCAAGGCGG GCATCATCGC CTCGGCCCGG GCCGGGGAGA CACGCTTCAC TGATACCCGG 
AAGGACGAGC AGGAGCGTTG CATCACCATC AAGTCAACTG CCATCTCCCT CTTCTACGAG 
CTCTCGGAGA ATGACTTGAA CTTCATCAAG CAGAGCAAGG ACGGTGCCGG CTTCCTCATC 
AACCTCATTG ACTCCCCCGG GCATGTCGAC TTCTCCTCGG AGGTGACTGC TGCCCTCCGA 
GTCACCGATG GCGCATTGGT GGTGGTGGAC TGCGTGTCAG GCGTGTGCGT GCAGACGGAG 
ACAGTGCTGC GGCAGGCCAT TGCCGAGCGC ATCAAGCCTG TGCTGATGAT GAACAAGATG 
GACCGCGCCC TGCTGGAGCT GCAGCTGGAG CCCGAGGAGC TCTACCAGAC TTTCCAGCGC 
ATCGTGGAGA ACGTGAACGT CATCATCTCC ACCTACGGCG AGGGCGAGAG CGGCCCCATG 
GGCAACATCA TGATCGATCC TGTCCTCGGT ACCGTGGGCT TTGGGTCTGG CCTCCACGGG 
TGGGCCTTCA CCCTGAAGCA GTTTGCCGAG ATGTATGTGG CCAAGTTCGC CGCCAAGGGG 
GAGGGCCAGT TGGGGCCTGC CGAGCGGGCC AAGAAAGTAG AGGACATGAT GAAGAAGCTG 
TGGGGTGACA GGTACTTTGA CCCAGCCAAC GGCAAGTTCA GCAAGTCAGC CACCAGCCCC 
GAAGGGAAGA AGCTGCCACG CACCTTCTGC CAGCTGATCC TGGACCCCAT CTTCAAGGTG 
TTTGATGCGA TCATGAATTT CAAGAAAGAG GAGACAGCAA AACTGATAGA GAAACTGGAC 
ATCAAACTGG ACAGCGAGGA CAAGGACAAA GAAGGCAAAC CCCTGCTGAA GGCTGTGATG 
CGCCGCTGGC TGCCTGCCGG AGACGCCTTG TTGCAGATGA TCACCATCCA CCTGCCCTCC 
CCTGTGACGG CCCAGAAGTA CCGCTGCGAG CTCCTGTACG AGGGGCCCCC GGACGACGAG 
GCTGCCATGG GCATTAAAAG CTGTGACCCC AAAGGCCCTC TTATGATGTA TATTTCCAAA 
ATGGTGCCAA CCTCCGACAA AGGTCGGTTC TACGCCTTTG GACGAGTCTT CTCGGGGCTG 
GTCTCCACTG GCCTGAAGGT CAGGATCATG GGGCCCAACT ATACCCCTGG GAAGAAGGAG 
GACCTCTACC TGAAGCCAAT CCAGAGAACA ATCTTGATGA TGGGCCGCTA CGTGGAGCCC 
ATCGAGGATG TGCCTTGTGG GAACATTGTG GGCCTCGTGG GCGTGGACCA GTTCCTGGTG 
AAGACGGGCA CCATCACCAC CTTCGAGCAC GCGCACAACA TGCGGGTGAT GAAGTTCAGC 
GTCAGCCCTG TTGTCAGAGT GGCCGTGGAG GCCAAGAACC CGGCTGACCT GCCCAAGCTG 
GTGGAGGGGC TGAAGCGGCT GGCCAAGTCC GACCCCATGG TGCAGTGCAT CATCGAGGAG 
TCGGGAGAGC ATATCATCGC GGGCGCCGGC GAGCTGCACC TGGAGATCTG CCTGAAGGAC 
CTGGAGGAGG ACCACGCCTG CATCCCCATC AAGAAATCTG ACCCGGTCGT CTCGTACCGC 
GAGACGGTCA GTGAAGAGTC GAACGTGCTC TGCCTCTCCA AGTCCCCCAA CAAGCACAAC 
CGGCTGTACA TGAAGGCGCG GCCCTTCCCC GACGGCCTGG CCGAGGACAT CGATAAAGGC 
GAGGTGTCCG CCCGTCAGGA GCTCAAGCAG CGGGCGCGCT ACCTGGCCGA GAAGTACGAG 
TGGGACGTGG CTGAGGCCCG CAAGATCTGG TGCTTTGGGC CCGACGGCAC CGGCCCCAAC 
ATCCTCACCG ACATCACCAA GGGTGTGCAG TACCTCAACG AGATCAAGGA CAGTGTGGTG 
GCCGGCTTCC AGTGGGCCAC CAAGGAGGGC GCACTGTGTG AGGAGAACAT GCGGGGTGTG 
CGCTTCGACG TCCACGACGT CACCCTGCAC GCCGACGCCA TCCACCGCGG AGGGGGCCAG 
ATCATCCCCA CAGCACGGCG CTGCCTCTAT GCCAGTGTGC TGACCGCCCA GCCACGCCTC 
ATGGAGCCCA TCTACCTTGT GGAGATCCAG TGTCCAGAGC AGGTGGTCGG TGGCATCTAC 
GGGGTTTTGA ACAGGAAGCG GGGCCACGTG TTCGAGGAGT CCCAGGTGGC CGGCACCCCC 
ATGTTTGTGG TCAAGGCCTA TCTGCCCGTC AACGAGTCCT TTGGCTTCAC CGCTGACCTG 
AGGTCCAACA CGGGCGGCCA GGCGTTCCCC CAGTGTGTGT TTGACCACTG GCAGATCCTG 
CCCGGAGACC CCTTCGACAA CAGCAGCCGC CCCAGCCAGG TGGTGGCGGA GACCCGCAAG 
CGCAAGGGCC TGAAAGAAGG CATCCCTGCC CTGGACAACT TCCTGGACAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001952.1
CDS	84..2660
Translation	MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGAGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLGPAERAKKVEDMMKKLWGDRYFDPANGKFSKSATSPEGKKLPRTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL

Target ORF information:

RefSeq Version	NM_001961.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation elongation factor 2 (EEF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001961.4

ORF Insert Sequence:

ATGGTGAACT TCACGGTAGA CCAGATCCGC GCCATCATGG ACAAGAAGGC CAACATCCGC 
AACATGTCTG TCATCGCCCA CGTGGACCAT GGCAAGTCCA CGCTGACAGA CTCCCTGGTG 
TGCAAGGCGG GCATCATCGC CTCGGCCCGG GCCGGGGAGA CACGCTTCAC TGATACCCGG 
AAGGACGAGC AGGAGCGTTG CATCACCATC AAGTCAACTG CCATCTCCCT CTTCTACGAG 
CTCTCGGAGA ATGACTTGAA CTTCATCAAG CAGAGCAAGG ACGGTGCCGG CTTCCTCATC 
AACCTCATTG ACTCCCCCGG GCATGTCGAC TTCTCCTCGG AGGTGACTGC TGCCCTCCGA 
GTCACCGATG GCGCATTGGT GGTGGTGGAC TGCGTGTCAG GCGTGTGCGT GCAGACGGAG 
ACAGTGCTGC GGCAGGCCAT TGCCGAGCGC ATCAAGCCTG TGCTGATGAT GAACAAGATG 
GACCGCGCCC TGCTGGAGCT GCAGCTGGAG CCCGAGGAGC TCTACCAGAC TTTCCAGCGC 
ATCGTGGAGA ACGTGAACGT CATCATCTCC ACCTACGGCG AGGGCGAGAG CGGCCCCATG 
GGCAACATCA TGATCGATCC TGTCCTCGGT ACCGTGGGCT TTGGGTCTGG CCTCCACGGG 
TGGGCCTTCA CCCTGAAGCA GTTTGCCGAG ATGTATGTGG CCAAGTTCGC CGCCAAGGGG 
GAGGGCCAGT TGGGGCCTGC CGAGCGGGCC AAGAAAGTAG AGGACATGAT GAAGAAGCTG 
TGGGGTGACA GGTACTTTGA CCCAGCCAAC GGCAAGTTCA GCAAGTCAGC CACCAGCCCC 
GAAGGGAAGA AGCTGCCACG CACCTTCTGC CAGCTGATCC TGGACCCCAT CTTCAAGGTG 
TTTGATGCGA TCATGAATTT CAAGAAAGAG GAGACAGCAA AACTGATAGA GAAACTGGAC 
ATCAAACTGG ACAGCGAGGA CAAGGACAAA GAAGGCAAAC CCCTGCTGAA GGCTGTGATG 
CGCCGCTGGC TGCCTGCCGG AGACGCCTTG TTGCAGATGA TCACCATCCA CCTGCCCTCC 
CCTGTGACGG CCCAGAAGTA CCGCTGCGAG CTCCTGTACG AGGGGCCCCC GGACGACGAG 
GCTGCCATGG GCATTAAAAG CTGTGACCCC AAAGGCCCTC TTATGATGTA TATTTCCAAA 
ATGGTGCCAA CCTCCGACAA AGGTCGGTTC TACGCCTTTG GACGAGTCTT CTCGGGGCTG 
GTCTCCACTG GCCTGAAGGT CAGGATCATG GGGCCCAACT ATACCCCTGG GAAGAAGGAG 
GACCTCTACC TGAAGCCAAT CCAGAGAACA ATCTTGATGA TGGGCCGCTA CGTGGAGCCC 
ATCGAGGATG TGCCTTGTGG GAACATTGTG GGCCTCGTGG GCGTGGACCA GTTCCTGGTG 
AAGACGGGCA CCATCACCAC CTTCGAGCAC GCGCACAACA TGCGGGTGAT GAAGTTCAGC 
GTCAGCCCTG TTGTCAGAGT GGCCGTGGAG GCCAAGAACC CGGCTGACCT GCCCAAGCTG 
GTGGAGGGGC TGAAGCGGCT GGCCAAGTCC GACCCCATGG TGCAGTGCAT CATCGAGGAG 
TCGGGAGAGC ATATCATCGC GGGCGCCGGC GAGCTGCACC TGGAGATCTG CCTGAAGGAC 
CTGGAGGAGG ACCACGCCTG CATCCCCATC AAGAAATCTG ACCCGGTCGT CTCGTACCGC 
GAGACGGTCA GTGAAGAGTC GAACGTGCTC TGCCTCTCCA AGTCCCCCAA CAAGCACAAC 
CGGCTGTACA TGAAGGCGCG GCCCTTCCCC GACGGCCTGG CCGAGGACAT CGATAAAGGC 
GAGGTGTCCG CCCGTCAGGA GCTCAAGCAG CGGGCGCGCT ACCTGGCCGA GAAGTACGAG 
TGGGACGTGG CTGAGGCCCG CAAGATCTGG TGCTTTGGGC CCGACGGCAC CGGCCCCAAC 
ATCCTCACCG ACATCACCAA GGGTGTGCAG TACCTCAACG AGATCAAGGA CAGTGTGGTG 
GCCGGCTTCC AGTGGGCCAC CAAGGAGGGC GCACTGTGTG AGGAGAACAT GCGGGGTGTG 
CGCTTCGACG TCCACGACGT CACCCTGCAC GCCGACGCCA TCCACCGCGG AGGGGGCCAG 
ATCATCCCCA CAGCACGGCG CTGCCTCTAT GCCAGTGTGC TGACCGCCCA GCCACGCCTC 
ATGGAGCCCA TCTACCTTGT GGAGATCCAG TGTCCAGAGC AGGTGGTCGG TGGCATCTAC 
GGGGTTTTGA ACAGGAAGCG GGGCCACGTG TTCGAGGAGT CCCAGGTGGC CGGCACCCCC 
ATGTTTGTGG TCAAGGCCTA TCTGCCCGTC AACGAGTCCT TTGGCTTCAC CGCTGACCTG 
AGGTCCAACA CGGGCGGCCA GGCGTTCCCC CAGTGTGTGT TTGACCACTG GCAGATCCTG 
CCCGGAGACC CCTTCGACAA CAGCAGCCGC CCCAGCCAGG TGGTGGCGGA GACCCGCAAG 
CGCAAGGGCC TGAAAGAAGG CATCCCTGCC CTGGACAACT TCCTGGACAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF2 ( NM_001961.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001952.1 Homo sapiens eukaryotic translation elongation factor 2 (EEF2), mRNA.

Price & Availability↑

CloneID	OHu25759
Clone ID Related Accession (Same CDS sequence)	NM_001961.4 , NM_001961.3
Accession Version	NM_001961.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	elongation factor 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from AU118535.1, AY942181.1 and Z11692.1. This sequence is a reference standard in the RefSeqGene project. On Dec 15, 2005 this sequence version replaced NM_001961.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.96317.1, SRR1163658.173334.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGTGAACT TCACGGTAGA CCAGATCCGC GCCATCATGG ACAAGAAGGC CAACATCCGC 
AACATGTCTG TCATCGCCCA CGTGGACCAT GGCAAGTCCA CGCTGACAGA CTCCCTGGTG 
TGCAAGGCGG GCATCATCGC CTCGGCCCGG GCCGGGGAGA CACGCTTCAC TGATACCCGG 
AAGGACGAGC AGGAGCGTTG CATCACCATC AAGTCAACTG CCATCTCCCT CTTCTACGAG 
CTCTCGGAGA ATGACTTGAA CTTCATCAAG CAGAGCAAGG ACGGTGCCGG CTTCCTCATC 
AACCTCATTG ACTCCCCCGG GCATGTCGAC TTCTCCTCGG AGGTGACTGC TGCCCTCCGA 
GTCACCGATG GCGCATTGGT GGTGGTGGAC TGCGTGTCAG GCGTGTGCGT GCAGACGGAG 
ACAGTGCTGC GGCAGGCCAT TGCCGAGCGC ATCAAGCCTG TGCTGATGAT GAACAAGATG 
GACCGCGCCC TGCTGGAGCT GCAGCTGGAG CCCGAGGAGC TCTACCAGAC TTTCCAGCGC 
ATCGTGGAGA ACGTGAACGT CATCATCTCC ACCTACGGCG AGGGCGAGAG CGGCCCCATG 
GGCAACATCA TGATCGATCC TGTCCTCGGT ACCGTGGGCT TTGGGTCTGG CCTCCACGGG 
TGGGCCTTCA CCCTGAAGCA GTTTGCCGAG ATGTATGTGG CCAAGTTCGC CGCCAAGGGG 
GAGGGCCAGT TGGGGCCTGC CGAGCGGGCC AAGAAAGTAG AGGACATGAT GAAGAAGCTG 
TGGGGTGACA GGTACTTTGA CCCAGCCAAC GGCAAGTTCA GCAAGTCAGC CACCAGCCCC 
GAAGGGAAGA AGCTGCCACG CACCTTCTGC CAGCTGATCC TGGACCCCAT CTTCAAGGTG 
TTTGATGCGA TCATGAATTT CAAGAAAGAG GAGACAGCAA AACTGATAGA GAAACTGGAC 
ATCAAACTGG ACAGCGAGGA CAAGGACAAA GAAGGCAAAC CCCTGCTGAA GGCTGTGATG 
CGCCGCTGGC TGCCTGCCGG AGACGCCTTG TTGCAGATGA TCACCATCCA CCTGCCCTCC 
CCTGTGACGG CCCAGAAGTA CCGCTGCGAG CTCCTGTACG AGGGGCCCCC GGACGACGAG 
GCTGCCATGG GCATTAAAAG CTGTGACCCC AAAGGCCCTC TTATGATGTA TATTTCCAAA 
ATGGTGCCAA CCTCCGACAA AGGTCGGTTC TACGCCTTTG GACGAGTCTT CTCGGGGCTG 
GTCTCCACTG GCCTGAAGGT CAGGATCATG GGGCCCAACT ATACCCCTGG GAAGAAGGAG 
GACCTCTACC TGAAGCCAAT CCAGAGAACA ATCTTGATGA TGGGCCGCTA CGTGGAGCCC 
ATCGAGGATG TGCCTTGTGG GAACATTGTG GGCCTCGTGG GCGTGGACCA GTTCCTGGTG 
AAGACGGGCA CCATCACCAC CTTCGAGCAC GCGCACAACA TGCGGGTGAT GAAGTTCAGC 
GTCAGCCCTG TTGTCAGAGT GGCCGTGGAG GCCAAGAACC CGGCTGACCT GCCCAAGCTG 
GTGGAGGGGC TGAAGCGGCT GGCCAAGTCC GACCCCATGG TGCAGTGCAT CATCGAGGAG 
TCGGGAGAGC ATATCATCGC GGGCGCCGGC GAGCTGCACC TGGAGATCTG CCTGAAGGAC 
CTGGAGGAGG ACCACGCCTG CATCCCCATC AAGAAATCTG ACCCGGTCGT CTCGTACCGC 
GAGACGGTCA GTGAAGAGTC GAACGTGCTC TGCCTCTCCA AGTCCCCCAA CAAGCACAAC 
CGGCTGTACA TGAAGGCGCG GCCCTTCCCC GACGGCCTGG CCGAGGACAT CGATAAAGGC 
GAGGTGTCCG CCCGTCAGGA GCTCAAGCAG CGGGCGCGCT ACCTGGCCGA GAAGTACGAG 
TGGGACGTGG CTGAGGCCCG CAAGATCTGG TGCTTTGGGC CCGACGGCAC CGGCCCCAAC 
ATCCTCACCG ACATCACCAA GGGTGTGCAG TACCTCAACG AGATCAAGGA CAGTGTGGTG 
GCCGGCTTCC AGTGGGCCAC CAAGGAGGGC GCACTGTGTG AGGAGAACAT GCGGGGTGTG 
CGCTTCGACG TCCACGACGT CACCCTGCAC GCCGACGCCA TCCACCGCGG AGGGGGCCAG 
ATCATCCCCA CAGCACGGCG CTGCCTCTAT GCCAGTGTGC TGACCGCCCA GCCACGCCTC 
ATGGAGCCCA TCTACCTTGT GGAGATCCAG TGTCCAGAGC AGGTGGTCGG TGGCATCTAC 
GGGGTTTTGA ACAGGAAGCG GGGCCACGTG TTCGAGGAGT CCCAGGTGGC CGGCACCCCC 
ATGTTTGTGG TCAAGGCCTA TCTGCCCGTC AACGAGTCCT TTGGCTTCAC CGCTGACCTG 
AGGTCCAACA CGGGCGGCCA GGCGTTCCCC CAGTGTGTGT TTGACCACTG GCAGATCCTG 
CCCGGAGACC CCTTCGACAA CAGCAGCCGC CCCAGCCAGG TGGTGGCGGA GACCCGCAAG 
CGCAAGGGCC TGAAAGAAGG CATCCCTGCC CTGGACAACT TCCTGGACAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001952.1
CDS	84..2660
Translation	MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGAGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLGPAERAKKVEDMMKKLWGDRYFDPANGKFSKSATSPEGKKLPRTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL

Target ORF information:

RefSeq Version	NM_001961.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation elongation factor 2 (EEF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001961.3

ORF Insert Sequence:

ATGGTGAACT TCACGGTAGA CCAGATCCGC GCCATCATGG ACAAGAAGGC CAACATCCGC 
AACATGTCTG TCATCGCCCA CGTGGACCAT GGCAAGTCCA CGCTGACAGA CTCCCTGGTG 
TGCAAGGCGG GCATCATCGC CTCGGCCCGG GCCGGGGAGA CACGCTTCAC TGATACCCGG 
AAGGACGAGC AGGAGCGTTG CATCACCATC AAGTCAACTG CCATCTCCCT CTTCTACGAG 
CTCTCGGAGA ATGACTTGAA CTTCATCAAG CAGAGCAAGG ACGGTGCCGG CTTCCTCATC 
AACCTCATTG ACTCCCCCGG GCATGTCGAC TTCTCCTCGG AGGTGACTGC TGCCCTCCGA 
GTCACCGATG GCGCATTGGT GGTGGTGGAC TGCGTGTCAG GCGTGTGCGT GCAGACGGAG 
ACAGTGCTGC GGCAGGCCAT TGCCGAGCGC ATCAAGCCTG TGCTGATGAT GAACAAGATG 
GACCGCGCCC TGCTGGAGCT GCAGCTGGAG CCCGAGGAGC TCTACCAGAC TTTCCAGCGC 
ATCGTGGAGA ACGTGAACGT CATCATCTCC ACCTACGGCG AGGGCGAGAG CGGCCCCATG 
GGCAACATCA TGATCGATCC TGTCCTCGGT ACCGTGGGCT TTGGGTCTGG CCTCCACGGG 
TGGGCCTTCA CCCTGAAGCA GTTTGCCGAG ATGTATGTGG CCAAGTTCGC CGCCAAGGGG 
GAGGGCCAGT TGGGGCCTGC CGAGCGGGCC AAGAAAGTAG AGGACATGAT GAAGAAGCTG 
TGGGGTGACA GGTACTTTGA CCCAGCCAAC GGCAAGTTCA GCAAGTCAGC CACCAGCCCC 
GAAGGGAAGA AGCTGCCACG CACCTTCTGC CAGCTGATCC TGGACCCCAT CTTCAAGGTG 
TTTGATGCGA TCATGAATTT CAAGAAAGAG GAGACAGCAA AACTGATAGA GAAACTGGAC 
ATCAAACTGG ACAGCGAGGA CAAGGACAAA GAAGGCAAAC CCCTGCTGAA GGCTGTGATG 
CGCCGCTGGC TGCCTGCCGG AGACGCCTTG TTGCAGATGA TCACCATCCA CCTGCCCTCC 
CCTGTGACGG CCCAGAAGTA CCGCTGCGAG CTCCTGTACG AGGGGCCCCC GGACGACGAG 
GCTGCCATGG GCATTAAAAG CTGTGACCCC AAAGGCCCTC TTATGATGTA TATTTCCAAA 
ATGGTGCCAA CCTCCGACAA AGGTCGGTTC TACGCCTTTG GACGAGTCTT CTCGGGGCTG 
GTCTCCACTG GCCTGAAGGT CAGGATCATG GGGCCCAACT ATACCCCTGG GAAGAAGGAG 
GACCTCTACC TGAAGCCAAT CCAGAGAACA ATCTTGATGA TGGGCCGCTA CGTGGAGCCC 
ATCGAGGATG TGCCTTGTGG GAACATTGTG GGCCTCGTGG GCGTGGACCA GTTCCTGGTG 
AAGACGGGCA CCATCACCAC CTTCGAGCAC GCGCACAACA TGCGGGTGAT GAAGTTCAGC 
GTCAGCCCTG TTGTCAGAGT GGCCGTGGAG GCCAAGAACC CGGCTGACCT GCCCAAGCTG 
GTGGAGGGGC TGAAGCGGCT GGCCAAGTCC GACCCCATGG TGCAGTGCAT CATCGAGGAG 
TCGGGAGAGC ATATCATCGC GGGCGCCGGC GAGCTGCACC TGGAGATCTG CCTGAAGGAC 
CTGGAGGAGG ACCACGCCTG CATCCCCATC AAGAAATCTG ACCCGGTCGT CTCGTACCGC 
GAGACGGTCA GTGAAGAGTC GAACGTGCTC TGCCTCTCCA AGTCCCCCAA CAAGCACAAC 
CGGCTGTACA TGAAGGCGCG GCCCTTCCCC GACGGCCTGG CCGAGGACAT CGATAAAGGC 
GAGGTGTCCG CCCGTCAGGA GCTCAAGCAG CGGGCGCGCT ACCTGGCCGA GAAGTACGAG 
TGGGACGTGG CTGAGGCCCG CAAGATCTGG TGCTTTGGGC CCGACGGCAC CGGCCCCAAC 
ATCCTCACCG ACATCACCAA GGGTGTGCAG TACCTCAACG AGATCAAGGA CAGTGTGGTG 
GCCGGCTTCC AGTGGGCCAC CAAGGAGGGC GCACTGTGTG AGGAGAACAT GCGGGGTGTG 
CGCTTCGACG TCCACGACGT CACCCTGCAC GCCGACGCCA TCCACCGCGG AGGGGGCCAG 
ATCATCCCCA CAGCACGGCG CTGCCTCTAT GCCAGTGTGC TGACCGCCCA GCCACGCCTC 
ATGGAGCCCA TCTACCTTGT GGAGATCCAG TGTCCAGAGC AGGTGGTCGG TGGCATCTAC 
GGGGTTTTGA ACAGGAAGCG GGGCCACGTG TTCGAGGAGT CCCAGGTGGC CGGCACCCCC 
ATGTTTGTGG TCAAGGCCTA TCTGCCCGTC AACGAGTCCT TTGGCTTCAC CGCTGACCTG 
AGGTCCAACA CGGGCGGCCA GGCGTTCCCC CAGTGTGTGT TTGACCACTG GCAGATCCTG 
CCCGGAGACC CCTTCGACAA CAGCAGCCGC CCCAGCCAGG TGGTGGCGGA GACCCGCAAG 
CGCAAGGGCC TGAAAGAAGG CATCCCTGCC CTGGACAACT TCCTGGACAA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling.
Cell systems5(6)564-577.e12(2017 Dec)
Malty RH,Aoki H,Kumar A,Phanse S,Amin S,Zhang Q,Minic Z,Goebels F,Musso G,Wu Z,Abou-Tok H,Meyer M,Deineko V,Kassir S,Sidhu V,Jessulat M,Scott NE,Xiong X,Vlasblom J,Prasad B,Foster LJ,Alberio T,Garavaglia B,Yu H,Bader GD,Nakamura K,Parkinson J,Babu M

Eukaryotic Elongation Factor 2 (eEF2) is a Potential Biomarker of Prostate Cancer.
Pathology oncology research : POR(2017 Sep)
Zhang X,Hu L,Du M,Wei X,Zhang J,Hui Y,Chen C,Li G,Hou J

Evidence for a Negative Cooperativity between eIF5A and eEF2 on Binding to the Ribosome.
PloS one11(4)e0154205(2016)
Rossi D,Barbosa NM,Galv?o FC,Boldrin PE,Hershey JW,Zanelli CF,Fraser CS,Valentini SR

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EEF2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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