eEF2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eEF2
Entrez Gene ID	35422
Full Name	Elongation factor 2
Synonyms	CG2238,Dmel\CG2238,EF-2,EF-2b,EF2B,EF2b,Ef2,Ef2B,Ef2b,anon-EST:Liang-1.44,chr2L:21668915..21669164,clone 1.44,eEF2,eF2,ef2b
General protein information	Preferred Names Elongation factor 2 Names CG2238 gene product from transcript CG2238-RD CG2238-PA CG2238-PC CG2238-PD EF2-PA EF2-PC EF2-PD ELONGATION FACTOR 2 elongation factor 2 elongation factor 2b eukaryotic translation elongation factor 2b
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 39E7-39E7

mRNA and Protein(s)

mRNA	Protein	Name
NM_165394.3	NP_724357.2	eukaryotic translation elongation factor 2, isoform D
NM_080366.3	NP_525105.2	eukaryotic translation elongation factor 2, isoform A
NM_165395.2	NP_724358.2	eukaryotic translation elongation factor 2, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC100614367	XP_003316060.2
	EEF2	XP_001118006.2
Drosophila melanogaster (fruit fly)	Ef2b	NP_525105.2
Mus musculus (house mouse)	Eef2	NP_031933.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009441	XP_307854.2
Danio rerio (zebrafish)	eef2b	NP_956752.2
Arabidopsis thaliana (thale cress)	LOS1	NP_849818.1
Oryza sativa (rice)	Os01g0742200	NP_001044211.1
Bos taurus (cattle)	EEF2	NP_001068589.1
Gallus gallus (chicken)	EEF2	NP_990699.1
Caenorhabditis elegans (roundworm)	eef-2	NP_001251010.1
Homo sapiens (human)	EEF2	NP_001952.1
Canis lupus familiaris (dog)	EEF2	XP_533949.3
Rattus norvegicus (Norway rat)	Eef2	NP_058941.1
Xenopus tropicalis (tropical clawed frog)	eef2.1	NP_989255.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following eEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15143	NM_165395.2 Latest version!	Drosophila melanogaster eukaryotic translation elongation factor 2 (eEF2), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa15143	NM_080366.3 Latest version!	Drosophila melanogaster eukaryotic translation elongation factor 2 (eEF2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa15143	NM_165394.3 Latest version!	Drosophila melanogaster eukaryotic translation elongation factor 2 (eEF2), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eEF2 ( NM_165394.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724357.2 Drosophila melanogaster eukaryotic translation elongation factor 2 (eEF2), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa15143
Clone ID Related Accession (Same CDS sequence)	NM_165394.3 , NM_080366.3 , NM_165395.2
Accession Version	NM_165394.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation elongation factor 2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165394.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCAACT TCACCGTCGA CGAGATCCGT GGCCTCATGG ACAAGAAGCG GAACATCCGC 
AACATGTCTG TGATTGCCCA CGTAGACCAC GGCAAGTCCA CTCTGACCGA TTCCCTTGTG 
TCGAAGGCTG GTATCATTGC CGGAGCCAAG GCTGGTGAGA CTCGTTTCAC TGACACCCGC 
AAGGACGAGC AGGAGCGCTG CATTACCATC AAGTCGACTG CCATTTCCAT GTACTTTGAG 
GTGGAGGAAA AGGATCTGGT GTTCATTACC CACCCCGATC AGCGCGAGAA GGAGTGCAAG 
GGTTTCCTGA TCAACTTGAT CGACTCGCCC GGTCACGTTG ACTTCTCCTC GGAGGTGACC 
GCCGCCCTTC GTGTAACCGA CGGTGCACTC GTTGTTGTAG ACTGCGTCTC CGGAGTTTGT 
GTCCAGACCG AAACCGTGCT GCGTCAGGCC ATCGCTGAGC GTATTAAGCC CATCCTATTC 
ATGAACAAAA TGGACCGCGC TCTGCTTGAG CTGCAACTAG ATGCCGAGGA GCTGTACCAG 
ACCTTCCAGC GCATCGTCGA GAACGTAAAC GTTATCATTG CCACCTATAA CGATGACGGA 
GGACCGATGG GTGAGGTTCG CGTGGATCCC TCTAAGGGAT CAGTGGGCTT CGGTTCTGGG 
CTTCACGGCT GGGCGTTCAC CCTCAAGCAG TTCTCTGAGA TGTACTCCGA GAAATTCAAG 
ATTGATGTTG TCAAGCTAAT GAACCGATTG TGGGGTGAAA ACTTCTTTAA CGCCAAGACC 
AAGAAATGGC AGAAACAGAA AGAGGCTGAC AACAAACGCT CGTTCTGCAT GTACATCCTG 
GACCCCATCT ACAAGGTGTT CGATGCCATT ATGAATTACA AGAAGGAGGA GATAGGTACC 
TTGCTCGAGA AAATCGGTGT GACTCTGAAG CATGAAGACA AGGACAAAGA TGGCAAAGCT 
CTGCTGAAGA CTGTGATGCG CACCTGGCTG CCAGCCGGCG AGGCTTTACT TCAGATGATT 
GCTATTCATT TGCCGTCGCC AGTGGTTGCC CAGAAGTACC GCATGGAGAT GCTGTACGAA 
GGTCCCCATG ACGATGAGGC CGCTATCGCC GTTAAGTCTT GCGATCCTGA TGGTCCTCTA 
ATGATGTACA TTTCTAAGAT GGTACCGACC TCTGACAAGG GTCGCTTCTA TGCTTTCGGT 
CGTGTGTTCG CCGGTAAGGT GGCCACCGGG CAAAAGTGCC GAATCATGGG CCCCAACTAC 
ACTCCCGGCA AGAAGGAGGA CTTATATGAG AAGGCTATTC AGCGCACAAT TCTCATGATG 
GGTCGTTACG TCGAGGCCAT CGAGGATGTG CCCTCTGGCA ACATTTGCGG TCTGGTCGGT 
GTTGATCAGT TCTTGGTTAA GACCGGTACG ATTACCACCT TCAAGGACGC CCACAACATG 
AAGGTCATGA AGTTCTCCGT GTCCCCTGTG GTGCGCGTGG CTGTTGAGCC AAAGAACCCC 
GCGGACTTGC CTAAGCTGGT GGAGGGCTTG AAGCGTTTGG CTAAGTCCGA TCCTATGGTG 
CAGTGTATCA TCGAAGAGTC CGGCGAGCAT ATCATTGCTG GTGCCGGTGA GCTGCATTTG 
GAGATCTGCC TTAAGGATCT AGAGGAAGAC CACGCTTGTA TCCCACTGAA GAAGTCTGAT 
CCCGTCGTCT CCTACCGTGA GACTGTGTCG GAGGAGTCCG ACCAGATGTG TCTGTCCAAG 
TCGCCCAACA AGCACAACCG TCTGCTGATG AAAGCCCTAC CTATGCCAGA CGGTCTTCCC 
GAGGACATTG ATAACGGCGA TGTGAGTGCC AAGGATGAGT TCAAGGCTCG TGCCCGTTAT 
TTGTCTGAGA AGTACGACTA CGATGTGACT GAGGCCCGTA AGATCTGGTG CTTCGGCCCC 
GACGGAACCG GTCCGAATTT CATCCTCGAC TGTACCAAGT CCGTGCAGTA CCTTAACGAA 
ATCAAGGACT CTGTTGTCGC TGGATTCCAA TGGGCCTCAA AGGAGGGTAT TTTAGCCGAC 
GAAAACTTGC GCGGTGTCCG CTTCAACATC TACGATGTGA CACTTCACGC TGATGCCATC 
CATCGTGGTG GTGGCCAGAT CATTCCAACC ACTCGTCGTT GTCTGTACGC TGCCGCTATA 
ACCGCCAAGC CGCGCCTGAT GGAGCCTGTT TACCTGTGCG AGATCCAGTG CCCCGAGGTT 
GCTGTAGGAG GAATATACGG TGTGCTCAAC AGGCGACGTG GCCATGTATT CGAGGAAAAC 
CAGGTTGTGG GCACTCCCAT GTTCGTTGTG AAGGCATACC TGCCCGTCAA CGAGTCATTC 
GGTTTTACCG CCGATCTGCG CTCTAATACC GGAGGACAGG CCTTCCCTCA ATGTGTCTTC 
GATCATTGGC AGGTGTTGCC TGGCGATCCG AGCGAGCCCT CAAGCAAGCC CTATGCCATC 
GTCCAGGATA CTCGTAAGCG CAAGGGTCTT AAGGAAGGAT TGCCCGATCT ATCCCAGTAC 
TTGGACAAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724357.2
CDS	76..2610
Translation	MVNFTVDEIRGLMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGAKAGETRFTDTRKDEQERCITIKSTAISMYFEVEEKDLVFITHPDQREKECKGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPILFMNKMDRALLELQLDAEELYQTFQRIVENVNVIIATYNDDGGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYSEKFKIDVVKLMNRLWGENFFNAKTKKWQKQKEADNKRSFCMYILDPIYKVFDAIMNYKKEEIGTLLEKIGVTLKHEDKDKDGKALLKTVMRTWLPAGEALLQMIAIHLPSPVVAQKYRMEMLYEGPHDDEAAIAVKSCDPDGPLMMYISKMVPTSDKGRFYAFGRVFAGKVATGQKCRIMGPNYTPGKKEDLYEKAIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFKDAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPLKKSDPVVSYRETVSEESDQMCLSKSPNKHNRLLMKALPMPDGLPEDIDNGDVSAKDEFKARARYLSEKYDYDVTEARKIWCFGPDGTGPNFILDCTKSVQYLNEIKDSVVAGFQWASKEGILADENLRGVRFNIYDVTLHADAIHRGGGQIIPTTRRCLYAAAITAKPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEENQVVGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPSEPSSKPYAIVQDTRKRKGLKEGLPDLSQYLDKL

Target ORF information:

RefSeq Version	NM_165394.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation elongation factor 2 (eEF2), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165394.3

ORF Insert Sequence:

ATGGTCAACT TCACCGTCGA CGAGATCCGT GGCCTCATGG ACAAGAAGCG GAACATCCGC 
AACATGTCTG TGATTGCCCA CGTAGACCAC GGCAAGTCCA CTCTGACCGA TTCCCTTGTG 
TCGAAGGCTG GTATCATTGC CGGAGCCAAG GCTGGTGAGA CTCGTTTCAC TGACACCCGC 
AAGGACGAGC AGGAGCGCTG CATTACCATC AAGTCGACTG CCATTTCCAT GTACTTTGAG 
GTGGAGGAAA AGGATCTGGT GTTCATTACC CACCCCGATC AGCGCGAGAA GGAGTGCAAG 
GGTTTCCTGA TCAACTTGAT CGACTCGCCC GGTCACGTTG ACTTCTCCTC GGAGGTGACC 
GCCGCCCTTC GTGTAACCGA CGGTGCACTC GTTGTTGTAG ACTGCGTCTC CGGAGTTTGT 
GTCCAGACCG AAACCGTGCT GCGTCAGGCC ATCGCTGAGC GTATTAAGCC CATCCTATTC 
ATGAACAAAA TGGACCGCGC TCTGCTTGAG CTGCAACTAG ATGCCGAGGA GCTGTACCAG 
ACCTTCCAGC GCATCGTCGA GAACGTAAAC GTTATCATTG CCACCTATAA CGATGACGGA 
GGACCGATGG GTGAGGTTCG CGTGGATCCC TCTAAGGGAT CAGTGGGCTT CGGTTCTGGG 
CTTCACGGCT GGGCGTTCAC CCTCAAGCAG TTCTCTGAGA TGTACTCCGA GAAATTCAAG 
ATTGATGTTG TCAAGCTAAT GAACCGATTG TGGGGTGAAA ACTTCTTTAA CGCCAAGACC 
AAGAAATGGC AGAAACAGAA AGAGGCTGAC AACAAACGCT CGTTCTGCAT GTACATCCTG 
GACCCCATCT ACAAGGTGTT CGATGCCATT ATGAATTACA AGAAGGAGGA GATAGGTACC 
TTGCTCGAGA AAATCGGTGT GACTCTGAAG CATGAAGACA AGGACAAAGA TGGCAAAGCT 
CTGCTGAAGA CTGTGATGCG CACCTGGCTG CCAGCCGGCG AGGCTTTACT TCAGATGATT 
GCTATTCATT TGCCGTCGCC AGTGGTTGCC CAGAAGTACC GCATGGAGAT GCTGTACGAA 
GGTCCCCATG ACGATGAGGC CGCTATCGCC GTTAAGTCTT GCGATCCTGA TGGTCCTCTA 
ATGATGTACA TTTCTAAGAT GGTACCGACC TCTGACAAGG GTCGCTTCTA TGCTTTCGGT 
CGTGTGTTCG CCGGTAAGGT GGCCACCGGG CAAAAGTGCC GAATCATGGG CCCCAACTAC 
ACTCCCGGCA AGAAGGAGGA CTTATATGAG AAGGCTATTC AGCGCACAAT TCTCATGATG 
GGTCGTTACG TCGAGGCCAT CGAGGATGTG CCCTCTGGCA ACATTTGCGG TCTGGTCGGT 
GTTGATCAGT TCTTGGTTAA GACCGGTACG ATTACCACCT TCAAGGACGC CCACAACATG 
AAGGTCATGA AGTTCTCCGT GTCCCCTGTG GTGCGCGTGG CTGTTGAGCC AAAGAACCCC 
GCGGACTTGC CTAAGCTGGT GGAGGGCTTG AAGCGTTTGG CTAAGTCCGA TCCTATGGTG 
CAGTGTATCA TCGAAGAGTC CGGCGAGCAT ATCATTGCTG GTGCCGGTGA GCTGCATTTG 
GAGATCTGCC TTAAGGATCT AGAGGAAGAC CACGCTTGTA TCCCACTGAA GAAGTCTGAT 
CCCGTCGTCT CCTACCGTGA GACTGTGTCG GAGGAGTCCG ACCAGATGTG TCTGTCCAAG 
TCGCCCAACA AGCACAACCG TCTGCTGATG AAAGCCCTAC CTATGCCAGA CGGTCTTCCC 
GAGGACATTG ATAACGGCGA TGTGAGTGCC AAGGATGAGT TCAAGGCTCG TGCCCGTTAT 
TTGTCTGAGA AGTACGACTA CGATGTGACT GAGGCCCGTA AGATCTGGTG CTTCGGCCCC 
GACGGAACCG GTCCGAATTT CATCCTCGAC TGTACCAAGT CCGTGCAGTA CCTTAACGAA 
ATCAAGGACT CTGTTGTCGC TGGATTCCAA TGGGCCTCAA AGGAGGGTAT TTTAGCCGAC 
GAAAACTTGC GCGGTGTCCG CTTCAACATC TACGATGTGA CACTTCACGC TGATGCCATC 
CATCGTGGTG GTGGCCAGAT CATTCCAACC ACTCGTCGTT GTCTGTACGC TGCCGCTATA 
ACCGCCAAGC CGCGCCTGAT GGAGCCTGTT TACCTGTGCG AGATCCAGTG CCCCGAGGTT 
GCTGTAGGAG GAATATACGG TGTGCTCAAC AGGCGACGTG GCCATGTATT CGAGGAAAAC 
CAGGTTGTGG GCACTCCCAT GTTCGTTGTG AAGGCATACC TGCCCGTCAA CGAGTCATTC 
GGTTTTACCG CCGATCTGCG CTCTAATACC GGAGGACAGG CCTTCCCTCA ATGTGTCTTC 
GATCATTGGC AGGTGTTGCC TGGCGATCCG AGCGAGCCCT CAAGCAAGCC CTATGCCATC 
GTCCAGGATA CTCGTAAGCG CAAGGGTCTT AAGGAAGGAT TGCCCGATCT ATCCCAGTAC 
TTGGACAAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eEF2 ( NM_080366.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525105.2 Drosophila melanogaster eukaryotic translation elongation factor 2 (eEF2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa15143
Clone ID Related Accession (Same CDS sequence)	NM_165394.3 , NM_080366.3 , NM_165395.2
Accession Version	NM_080366.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation elongation factor 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_080366.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCAACT TCACCGTCGA CGAGATCCGT GGCCTCATGG ACAAGAAGCG GAACATCCGC 
AACATGTCTG TGATTGCCCA CGTAGACCAC GGCAAGTCCA CTCTGACCGA TTCCCTTGTG 
TCGAAGGCTG GTATCATTGC CGGAGCCAAG GCTGGTGAGA CTCGTTTCAC TGACACCCGC 
AAGGACGAGC AGGAGCGCTG CATTACCATC AAGTCGACTG CCATTTCCAT GTACTTTGAG 
GTGGAGGAAA AGGATCTGGT GTTCATTACC CACCCCGATC AGCGCGAGAA GGAGTGCAAG 
GGTTTCCTGA TCAACTTGAT CGACTCGCCC GGTCACGTTG ACTTCTCCTC GGAGGTGACC 
GCCGCCCTTC GTGTAACCGA CGGTGCACTC GTTGTTGTAG ACTGCGTCTC CGGAGTTTGT 
GTCCAGACCG AAACCGTGCT GCGTCAGGCC ATCGCTGAGC GTATTAAGCC CATCCTATTC 
ATGAACAAAA TGGACCGCGC TCTGCTTGAG CTGCAACTAG ATGCCGAGGA GCTGTACCAG 
ACCTTCCAGC GCATCGTCGA GAACGTAAAC GTTATCATTG CCACCTATAA CGATGACGGA 
GGACCGATGG GTGAGGTTCG CGTGGATCCC TCTAAGGGAT CAGTGGGCTT CGGTTCTGGG 
CTTCACGGCT GGGCGTTCAC CCTCAAGCAG TTCTCTGAGA TGTACTCCGA GAAATTCAAG 
ATTGATGTTG TCAAGCTAAT GAACCGATTG TGGGGTGAAA ACTTCTTTAA CGCCAAGACC 
AAGAAATGGC AGAAACAGAA AGAGGCTGAC AACAAACGCT CGTTCTGCAT GTACATCCTG 
GACCCCATCT ACAAGGTGTT CGATGCCATT ATGAATTACA AGAAGGAGGA GATAGGTACC 
TTGCTCGAGA AAATCGGTGT GACTCTGAAG CATGAAGACA AGGACAAAGA TGGCAAAGCT 
CTGCTGAAGA CTGTGATGCG CACCTGGCTG CCAGCCGGCG AGGCTTTACT TCAGATGATT 
GCTATTCATT TGCCGTCGCC AGTGGTTGCC CAGAAGTACC GCATGGAGAT GCTGTACGAA 
GGTCCCCATG ACGATGAGGC CGCTATCGCC GTTAAGTCTT GCGATCCTGA TGGTCCTCTA 
ATGATGTACA TTTCTAAGAT GGTACCGACC TCTGACAAGG GTCGCTTCTA TGCTTTCGGT 
CGTGTGTTCG CCGGTAAGGT GGCCACCGGG CAAAAGTGCC GAATCATGGG CCCCAACTAC 
ACTCCCGGCA AGAAGGAGGA CTTATATGAG AAGGCTATTC AGCGCACAAT TCTCATGATG 
GGTCGTTACG TCGAGGCCAT CGAGGATGTG CCCTCTGGCA ACATTTGCGG TCTGGTCGGT 
GTTGATCAGT TCTTGGTTAA GACCGGTACG ATTACCACCT TCAAGGACGC CCACAACATG 
AAGGTCATGA AGTTCTCCGT GTCCCCTGTG GTGCGCGTGG CTGTTGAGCC AAAGAACCCC 
GCGGACTTGC CTAAGCTGGT GGAGGGCTTG AAGCGTTTGG CTAAGTCCGA TCCTATGGTG 
CAGTGTATCA TCGAAGAGTC CGGCGAGCAT ATCATTGCTG GTGCCGGTGA GCTGCATTTG 
GAGATCTGCC TTAAGGATCT AGAGGAAGAC CACGCTTGTA TCCCACTGAA GAAGTCTGAT 
CCCGTCGTCT CCTACCGTGA GACTGTGTCG GAGGAGTCCG ACCAGATGTG TCTGTCCAAG 
TCGCCCAACA AGCACAACCG TCTGCTGATG AAAGCCCTAC CTATGCCAGA CGGTCTTCCC 
GAGGACATTG ATAACGGCGA TGTGAGTGCC AAGGATGAGT TCAAGGCTCG TGCCCGTTAT 
TTGTCTGAGA AGTACGACTA CGATGTGACT GAGGCCCGTA AGATCTGGTG CTTCGGCCCC 
GACGGAACCG GTCCGAATTT CATCCTCGAC TGTACCAAGT CCGTGCAGTA CCTTAACGAA 
ATCAAGGACT CTGTTGTCGC TGGATTCCAA TGGGCCTCAA AGGAGGGTAT TTTAGCCGAC 
GAAAACTTGC GCGGTGTCCG CTTCAACATC TACGATGTGA CACTTCACGC TGATGCCATC 
CATCGTGGTG GTGGCCAGAT CATTCCAACC ACTCGTCGTT GTCTGTACGC TGCCGCTATA 
ACCGCCAAGC CGCGCCTGAT GGAGCCTGTT TACCTGTGCG AGATCCAGTG CCCCGAGGTT 
GCTGTAGGAG GAATATACGG TGTGCTCAAC AGGCGACGTG GCCATGTATT CGAGGAAAAC 
CAGGTTGTGG GCACTCCCAT GTTCGTTGTG AAGGCATACC TGCCCGTCAA CGAGTCATTC 
GGTTTTACCG CCGATCTGCG CTCTAATACC GGAGGACAGG CCTTCCCTCA ATGTGTCTTC 
GATCATTGGC AGGTGTTGCC TGGCGATCCG AGCGAGCCCT CAAGCAAGCC CTATGCCATC 
GTCCAGGATA CTCGTAAGCG CAAGGGTCTT AAGGAAGGAT TGCCCGATCT ATCCCAGTAC 
TTGGACAAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525105.2
CDS	76..2610
Translation	MVNFTVDEIRGLMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGAKAGETRFTDTRKDEQERCITIKSTAISMYFEVEEKDLVFITHPDQREKECKGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPILFMNKMDRALLELQLDAEELYQTFQRIVENVNVIIATYNDDGGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYSEKFKIDVVKLMNRLWGENFFNAKTKKWQKQKEADNKRSFCMYILDPIYKVFDAIMNYKKEEIGTLLEKIGVTLKHEDKDKDGKALLKTVMRTWLPAGEALLQMIAIHLPSPVVAQKYRMEMLYEGPHDDEAAIAVKSCDPDGPLMMYISKMVPTSDKGRFYAFGRVFAGKVATGQKCRIMGPNYTPGKKEDLYEKAIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFKDAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPLKKSDPVVSYRETVSEESDQMCLSKSPNKHNRLLMKALPMPDGLPEDIDNGDVSAKDEFKARARYLSEKYDYDVTEARKIWCFGPDGTGPNFILDCTKSVQYLNEIKDSVVAGFQWASKEGILADENLRGVRFNIYDVTLHADAIHRGGGQIIPTTRRCLYAAAITAKPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEENQVVGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPSEPSSKPYAIVQDTRKRKGLKEGLPDLSQYLDKL

Target ORF information:

RefSeq Version	NM_080366.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation elongation factor 2 (eEF2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080366.3

ORF Insert Sequence:

ATGGTCAACT TCACCGTCGA CGAGATCCGT GGCCTCATGG ACAAGAAGCG GAACATCCGC 
AACATGTCTG TGATTGCCCA CGTAGACCAC GGCAAGTCCA CTCTGACCGA TTCCCTTGTG 
TCGAAGGCTG GTATCATTGC CGGAGCCAAG GCTGGTGAGA CTCGTTTCAC TGACACCCGC 
AAGGACGAGC AGGAGCGCTG CATTACCATC AAGTCGACTG CCATTTCCAT GTACTTTGAG 
GTGGAGGAAA AGGATCTGGT GTTCATTACC CACCCCGATC AGCGCGAGAA GGAGTGCAAG 
GGTTTCCTGA TCAACTTGAT CGACTCGCCC GGTCACGTTG ACTTCTCCTC GGAGGTGACC 
GCCGCCCTTC GTGTAACCGA CGGTGCACTC GTTGTTGTAG ACTGCGTCTC CGGAGTTTGT 
GTCCAGACCG AAACCGTGCT GCGTCAGGCC ATCGCTGAGC GTATTAAGCC CATCCTATTC 
ATGAACAAAA TGGACCGCGC TCTGCTTGAG CTGCAACTAG ATGCCGAGGA GCTGTACCAG 
ACCTTCCAGC GCATCGTCGA GAACGTAAAC GTTATCATTG CCACCTATAA CGATGACGGA 
GGACCGATGG GTGAGGTTCG CGTGGATCCC TCTAAGGGAT CAGTGGGCTT CGGTTCTGGG 
CTTCACGGCT GGGCGTTCAC CCTCAAGCAG TTCTCTGAGA TGTACTCCGA GAAATTCAAG 
ATTGATGTTG TCAAGCTAAT GAACCGATTG TGGGGTGAAA ACTTCTTTAA CGCCAAGACC 
AAGAAATGGC AGAAACAGAA AGAGGCTGAC AACAAACGCT CGTTCTGCAT GTACATCCTG 
GACCCCATCT ACAAGGTGTT CGATGCCATT ATGAATTACA AGAAGGAGGA GATAGGTACC 
TTGCTCGAGA AAATCGGTGT GACTCTGAAG CATGAAGACA AGGACAAAGA TGGCAAAGCT 
CTGCTGAAGA CTGTGATGCG CACCTGGCTG CCAGCCGGCG AGGCTTTACT TCAGATGATT 
GCTATTCATT TGCCGTCGCC AGTGGTTGCC CAGAAGTACC GCATGGAGAT GCTGTACGAA 
GGTCCCCATG ACGATGAGGC CGCTATCGCC GTTAAGTCTT GCGATCCTGA TGGTCCTCTA 
ATGATGTACA TTTCTAAGAT GGTACCGACC TCTGACAAGG GTCGCTTCTA TGCTTTCGGT 
CGTGTGTTCG CCGGTAAGGT GGCCACCGGG CAAAAGTGCC GAATCATGGG CCCCAACTAC 
ACTCCCGGCA AGAAGGAGGA CTTATATGAG AAGGCTATTC AGCGCACAAT TCTCATGATG 
GGTCGTTACG TCGAGGCCAT CGAGGATGTG CCCTCTGGCA ACATTTGCGG TCTGGTCGGT 
GTTGATCAGT TCTTGGTTAA GACCGGTACG ATTACCACCT TCAAGGACGC CCACAACATG 
AAGGTCATGA AGTTCTCCGT GTCCCCTGTG GTGCGCGTGG CTGTTGAGCC AAAGAACCCC 
GCGGACTTGC CTAAGCTGGT GGAGGGCTTG AAGCGTTTGG CTAAGTCCGA TCCTATGGTG 
CAGTGTATCA TCGAAGAGTC CGGCGAGCAT ATCATTGCTG GTGCCGGTGA GCTGCATTTG 
GAGATCTGCC TTAAGGATCT AGAGGAAGAC CACGCTTGTA TCCCACTGAA GAAGTCTGAT 
CCCGTCGTCT CCTACCGTGA GACTGTGTCG GAGGAGTCCG ACCAGATGTG TCTGTCCAAG 
TCGCCCAACA AGCACAACCG TCTGCTGATG AAAGCCCTAC CTATGCCAGA CGGTCTTCCC 
GAGGACATTG ATAACGGCGA TGTGAGTGCC AAGGATGAGT TCAAGGCTCG TGCCCGTTAT 
TTGTCTGAGA AGTACGACTA CGATGTGACT GAGGCCCGTA AGATCTGGTG CTTCGGCCCC 
GACGGAACCG GTCCGAATTT CATCCTCGAC TGTACCAAGT CCGTGCAGTA CCTTAACGAA 
ATCAAGGACT CTGTTGTCGC TGGATTCCAA TGGGCCTCAA AGGAGGGTAT TTTAGCCGAC 
GAAAACTTGC GCGGTGTCCG CTTCAACATC TACGATGTGA CACTTCACGC TGATGCCATC 
CATCGTGGTG GTGGCCAGAT CATTCCAACC ACTCGTCGTT GTCTGTACGC TGCCGCTATA 
ACCGCCAAGC CGCGCCTGAT GGAGCCTGTT TACCTGTGCG AGATCCAGTG CCCCGAGGTT 
GCTGTAGGAG GAATATACGG TGTGCTCAAC AGGCGACGTG GCCATGTATT CGAGGAAAAC 
CAGGTTGTGG GCACTCCCAT GTTCGTTGTG AAGGCATACC TGCCCGTCAA CGAGTCATTC 
GGTTTTACCG CCGATCTGCG CTCTAATACC GGAGGACAGG CCTTCCCTCA ATGTGTCTTC 
GATCATTGGC AGGTGTTGCC TGGCGATCCG AGCGAGCCCT CAAGCAAGCC CTATGCCATC 
GTCCAGGATA CTCGTAAGCG CAAGGGTCTT AAGGAAGGAT TGCCCGATCT ATCCCAGTAC 
TTGGACAAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eEF2 ( NM_165395.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724358.2 Drosophila melanogaster eukaryotic translation elongation factor 2 (eEF2), transcript variant C, mRNA.

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CloneID	OFa15143
Clone ID Related Accession (Same CDS sequence)	NM_165394.3 , NM_080366.3 , NM_165395.2
Accession Version	NM_165395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation elongation factor 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165395.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCAACT TCACCGTCGA CGAGATCCGT GGCCTCATGG ACAAGAAGCG GAACATCCGC 
AACATGTCTG TGATTGCCCA CGTAGACCAC GGCAAGTCCA CTCTGACCGA TTCCCTTGTG 
TCGAAGGCTG GTATCATTGC CGGAGCCAAG GCTGGTGAGA CTCGTTTCAC TGACACCCGC 
AAGGACGAGC AGGAGCGCTG CATTACCATC AAGTCGACTG CCATTTCCAT GTACTTTGAG 
GTGGAGGAAA AGGATCTGGT GTTCATTACC CACCCCGATC AGCGCGAGAA GGAGTGCAAG 
GGTTTCCTGA TCAACTTGAT CGACTCGCCC GGTCACGTTG ACTTCTCCTC GGAGGTGACC 
GCCGCCCTTC GTGTAACCGA CGGTGCACTC GTTGTTGTAG ACTGCGTCTC CGGAGTTTGT 
GTCCAGACCG AAACCGTGCT GCGTCAGGCC ATCGCTGAGC GTATTAAGCC CATCCTATTC 
ATGAACAAAA TGGACCGCGC TCTGCTTGAG CTGCAACTAG ATGCCGAGGA GCTGTACCAG 
ACCTTCCAGC GCATCGTCGA GAACGTAAAC GTTATCATTG CCACCTATAA CGATGACGGA 
GGACCGATGG GTGAGGTTCG CGTGGATCCC TCTAAGGGAT CAGTGGGCTT CGGTTCTGGG 
CTTCACGGCT GGGCGTTCAC CCTCAAGCAG TTCTCTGAGA TGTACTCCGA GAAATTCAAG 
ATTGATGTTG TCAAGCTAAT GAACCGATTG TGGGGTGAAA ACTTCTTTAA CGCCAAGACC 
AAGAAATGGC AGAAACAGAA AGAGGCTGAC AACAAACGCT CGTTCTGCAT GTACATCCTG 
GACCCCATCT ACAAGGTGTT CGATGCCATT ATGAATTACA AGAAGGAGGA GATAGGTACC 
TTGCTCGAGA AAATCGGTGT GACTCTGAAG CATGAAGACA AGGACAAAGA TGGCAAAGCT 
CTGCTGAAGA CTGTGATGCG CACCTGGCTG CCAGCCGGCG AGGCTTTACT TCAGATGATT 
GCTATTCATT TGCCGTCGCC AGTGGTTGCC CAGAAGTACC GCATGGAGAT GCTGTACGAA 
GGTCCCCATG ACGATGAGGC CGCTATCGCC GTTAAGTCTT GCGATCCTGA TGGTCCTCTA 
ATGATGTACA TTTCTAAGAT GGTACCGACC TCTGACAAGG GTCGCTTCTA TGCTTTCGGT 
CGTGTGTTCG CCGGTAAGGT GGCCACCGGG CAAAAGTGCC GAATCATGGG CCCCAACTAC 
ACTCCCGGCA AGAAGGAGGA CTTATATGAG AAGGCTATTC AGCGCACAAT TCTCATGATG 
GGTCGTTACG TCGAGGCCAT CGAGGATGTG CCCTCTGGCA ACATTTGCGG TCTGGTCGGT 
GTTGATCAGT TCTTGGTTAA GACCGGTACG ATTACCACCT TCAAGGACGC CCACAACATG 
AAGGTCATGA AGTTCTCCGT GTCCCCTGTG GTGCGCGTGG CTGTTGAGCC AAAGAACCCC 
GCGGACTTGC CTAAGCTGGT GGAGGGCTTG AAGCGTTTGG CTAAGTCCGA TCCTATGGTG 
CAGTGTATCA TCGAAGAGTC CGGCGAGCAT ATCATTGCTG GTGCCGGTGA GCTGCATTTG 
GAGATCTGCC TTAAGGATCT AGAGGAAGAC CACGCTTGTA TCCCACTGAA GAAGTCTGAT 
CCCGTCGTCT CCTACCGTGA GACTGTGTCG GAGGAGTCCG ACCAGATGTG TCTGTCCAAG 
TCGCCCAACA AGCACAACCG TCTGCTGATG AAAGCCCTAC CTATGCCAGA CGGTCTTCCC 
GAGGACATTG ATAACGGCGA TGTGAGTGCC AAGGATGAGT TCAAGGCTCG TGCCCGTTAT 
TTGTCTGAGA AGTACGACTA CGATGTGACT GAGGCCCGTA AGATCTGGTG CTTCGGCCCC 
GACGGAACCG GTCCGAATTT CATCCTCGAC TGTACCAAGT CCGTGCAGTA CCTTAACGAA 
ATCAAGGACT CTGTTGTCGC TGGATTCCAA TGGGCCTCAA AGGAGGGTAT TTTAGCCGAC 
GAAAACTTGC GCGGTGTCCG CTTCAACATC TACGATGTGA CACTTCACGC TGATGCCATC 
CATCGTGGTG GTGGCCAGAT CATTCCAACC ACTCGTCGTT GTCTGTACGC TGCCGCTATA 
ACCGCCAAGC CGCGCCTGAT GGAGCCTGTT TACCTGTGCG AGATCCAGTG CCCCGAGGTT 
GCTGTAGGAG GAATATACGG TGTGCTCAAC AGGCGACGTG GCCATGTATT CGAGGAAAAC 
CAGGTTGTGG GCACTCCCAT GTTCGTTGTG AAGGCATACC TGCCCGTCAA CGAGTCATTC 
GGTTTTACCG CCGATCTGCG CTCTAATACC GGAGGACAGG CCTTCCCTCA ATGTGTCTTC 
GATCATTGGC AGGTGTTGCC TGGCGATCCG AGCGAGCCCT CAAGCAAGCC CTATGCCATC 
GTCCAGGATA CTCGTAAGCG CAAGGGTCTT AAGGAAGGAT TGCCCGATCT ATCCCAGTAC 
TTGGACAAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724358.2
CDS	124..2658
Translation	MVNFTVDEIRGLMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGAKAGETRFTDTRKDEQERCITIKSTAISMYFEVEEKDLVFITHPDQREKECKGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPILFMNKMDRALLELQLDAEELYQTFQRIVENVNVIIATYNDDGGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYSEKFKIDVVKLMNRLWGENFFNAKTKKWQKQKEADNKRSFCMYILDPIYKVFDAIMNYKKEEIGTLLEKIGVTLKHEDKDKDGKALLKTVMRTWLPAGEALLQMIAIHLPSPVVAQKYRMEMLYEGPHDDEAAIAVKSCDPDGPLMMYISKMVPTSDKGRFYAFGRVFAGKVATGQKCRIMGPNYTPGKKEDLYEKAIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFKDAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPLKKSDPVVSYRETVSEESDQMCLSKSPNKHNRLLMKALPMPDGLPEDIDNGDVSAKDEFKARARYLSEKYDYDVTEARKIWCFGPDGTGPNFILDCTKSVQYLNEIKDSVVAGFQWASKEGILADENLRGVRFNIYDVTLHADAIHRGGGQIIPTTRRCLYAAAITAKPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEENQVVGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPSEPSSKPYAIVQDTRKRKGLKEGLPDLSQYLDKL

Target ORF information:

RefSeq Version	NM_165395.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation elongation factor 2 (eEF2), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165395.2

ORF Insert Sequence:

ATGGTCAACT TCACCGTCGA CGAGATCCGT GGCCTCATGG ACAAGAAGCG GAACATCCGC 
AACATGTCTG TGATTGCCCA CGTAGACCAC GGCAAGTCCA CTCTGACCGA TTCCCTTGTG 
TCGAAGGCTG GTATCATTGC CGGAGCCAAG GCTGGTGAGA CTCGTTTCAC TGACACCCGC 
AAGGACGAGC AGGAGCGCTG CATTACCATC AAGTCGACTG CCATTTCCAT GTACTTTGAG 
GTGGAGGAAA AGGATCTGGT GTTCATTACC CACCCCGATC AGCGCGAGAA GGAGTGCAAG 
GGTTTCCTGA TCAACTTGAT CGACTCGCCC GGTCACGTTG ACTTCTCCTC GGAGGTGACC 
GCCGCCCTTC GTGTAACCGA CGGTGCACTC GTTGTTGTAG ACTGCGTCTC CGGAGTTTGT 
GTCCAGACCG AAACCGTGCT GCGTCAGGCC ATCGCTGAGC GTATTAAGCC CATCCTATTC 
ATGAACAAAA TGGACCGCGC TCTGCTTGAG CTGCAACTAG ATGCCGAGGA GCTGTACCAG 
ACCTTCCAGC GCATCGTCGA GAACGTAAAC GTTATCATTG CCACCTATAA CGATGACGGA 
GGACCGATGG GTGAGGTTCG CGTGGATCCC TCTAAGGGAT CAGTGGGCTT CGGTTCTGGG 
CTTCACGGCT GGGCGTTCAC CCTCAAGCAG TTCTCTGAGA TGTACTCCGA GAAATTCAAG 
ATTGATGTTG TCAAGCTAAT GAACCGATTG TGGGGTGAAA ACTTCTTTAA CGCCAAGACC 
AAGAAATGGC AGAAACAGAA AGAGGCTGAC AACAAACGCT CGTTCTGCAT GTACATCCTG 
GACCCCATCT ACAAGGTGTT CGATGCCATT ATGAATTACA AGAAGGAGGA GATAGGTACC 
TTGCTCGAGA AAATCGGTGT GACTCTGAAG CATGAAGACA AGGACAAAGA TGGCAAAGCT 
CTGCTGAAGA CTGTGATGCG CACCTGGCTG CCAGCCGGCG AGGCTTTACT TCAGATGATT 
GCTATTCATT TGCCGTCGCC AGTGGTTGCC CAGAAGTACC GCATGGAGAT GCTGTACGAA 
GGTCCCCATG ACGATGAGGC CGCTATCGCC GTTAAGTCTT GCGATCCTGA TGGTCCTCTA 
ATGATGTACA TTTCTAAGAT GGTACCGACC TCTGACAAGG GTCGCTTCTA TGCTTTCGGT 
CGTGTGTTCG CCGGTAAGGT GGCCACCGGG CAAAAGTGCC GAATCATGGG CCCCAACTAC 
ACTCCCGGCA AGAAGGAGGA CTTATATGAG AAGGCTATTC AGCGCACAAT TCTCATGATG 
GGTCGTTACG TCGAGGCCAT CGAGGATGTG CCCTCTGGCA ACATTTGCGG TCTGGTCGGT 
GTTGATCAGT TCTTGGTTAA GACCGGTACG ATTACCACCT TCAAGGACGC CCACAACATG 
AAGGTCATGA AGTTCTCCGT GTCCCCTGTG GTGCGCGTGG CTGTTGAGCC AAAGAACCCC 
GCGGACTTGC CTAAGCTGGT GGAGGGCTTG AAGCGTTTGG CTAAGTCCGA TCCTATGGTG 
CAGTGTATCA TCGAAGAGTC CGGCGAGCAT ATCATTGCTG GTGCCGGTGA GCTGCATTTG 
GAGATCTGCC TTAAGGATCT AGAGGAAGAC CACGCTTGTA TCCCACTGAA GAAGTCTGAT 
CCCGTCGTCT CCTACCGTGA GACTGTGTCG GAGGAGTCCG ACCAGATGTG TCTGTCCAAG 
TCGCCCAACA AGCACAACCG TCTGCTGATG AAAGCCCTAC CTATGCCAGA CGGTCTTCCC 
GAGGACATTG ATAACGGCGA TGTGAGTGCC AAGGATGAGT TCAAGGCTCG TGCCCGTTAT 
TTGTCTGAGA AGTACGACTA CGATGTGACT GAGGCCCGTA AGATCTGGTG CTTCGGCCCC 
GACGGAACCG GTCCGAATTT CATCCTCGAC TGTACCAAGT CCGTGCAGTA CCTTAACGAA 
ATCAAGGACT CTGTTGTCGC TGGATTCCAA TGGGCCTCAA AGGAGGGTAT TTTAGCCGAC 
GAAAACTTGC GCGGTGTCCG CTTCAACATC TACGATGTGA CACTTCACGC TGATGCCATC 
CATCGTGGTG GTGGCCAGAT CATTCCAACC ACTCGTCGTT GTCTGTACGC TGCCGCTATA 
ACCGCCAAGC CGCGCCTGAT GGAGCCTGTT TACCTGTGCG AGATCCAGTG CCCCGAGGTT 
GCTGTAGGAG GAATATACGG TGTGCTCAAC AGGCGACGTG GCCATGTATT CGAGGAAAAC 
CAGGTTGTGG GCACTCCCAT GTTCGTTGTG AAGGCATACC TGCCCGTCAA CGAGTCATTC 
GGTTTTACCG CCGATCTGCG CTCTAATACC GGAGGACAGG CCTTCCCTCA ATGTGTCTTC 
GATCATTGGC AGGTGTTGCC TGGCGATCCG AGCGAGCCCT CAAGCAAGCC CTATGCCATC 
GTCCAGGATA CTCGTAAGCG CAAGGGTCTT AAGGAAGGAT TGCCCGATCT ATCCCAGTAC 
TTGGACAAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eEF2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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