Eef2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eef2
Entrez Gene ID	29565
Full Name	eukaryotic translation elongation factor 2
Synonyms	Ef-2
General protein information	Preferred Names eukaryotic translation elongation factor 2 Names elongation factor 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11
Summary	catalyzes the translocation of peptidyl-tRNA from the ribosomal A site to the P site in translational elongation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017245.2	NP_058941.1	elongation factor 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC100614367	XP_003316060.2
	EEF2	XP_001118006.2
Drosophila melanogaster (fruit fly)	Ef2b	NP_525105.2
Mus musculus (house mouse)	Eef2	NP_031933.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009441	XP_307854.2
Danio rerio (zebrafish)	eef2b	NP_956752.2
Arabidopsis thaliana (thale cress)	LOS1	NP_849818.1
Oryza sativa (rice)	Os01g0742200	NP_001044211.1
Bos taurus (cattle)	EEF2	NP_001068589.1
Gallus gallus (chicken)	EEF2	NP_990699.1
Caenorhabditis elegans (roundworm)	eef-2	NP_001251010.1
Homo sapiens (human)	EEF2	NP_001952.1
Canis lupus familiaris (dog)	EEF2	XP_533949.3
Rattus norvegicus (Norway rat)	Eef2	NP_058941.1
Xenopus tropicalis (tropical clawed frog)	eef2.1	NP_989255.1

Gene Ontology
Bibliography

Related articles in PubMed

Eukaryotic elongation factor 2 kinase activation in M. soleus under 14-day hindlimb unloading of rats.
Lomonosova YN, Belova SP, Mirzoev TM, Kozlovskaya IB, Shenkman BS
Doklady. Biochemistry and biophysics474(1)165-167(2017 May)

Aging and Oxidative Stress Decrease Pineal Elongation Factor 2: In Vivo Protective Effect of Melatonin in Young Rats Treated With Cumene Hydroperoxide.
Mu?oz MF, Arg?elles S, Cano M, Marotta F, Ayala A
Journal of cellular biochemistry118(1)182-190(2017 Jan)

The mechanics of ribosomal translocation.
Achenbach J, Nierhaus KH
Biochimie11480-9(2015 Jul)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

Homodimerization of RBPMS2 through a new RRM-interaction motif is necessary to control smooth muscle plasticity.
Sagnol S, Yang Y, Bessin Y, Allemand F, Hapkova I, Notarnicola C, Guichou JF, Faure S, Labesse G, de Santa Barbara P
Nucleic acids research42(15)10173-84(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Eukaryotic elongation factor 2 kinase activation in M. soleus under 14-day hindlimb unloading of rats
Title: Eukaryotic elongation factor 2 kinase activation in M. soleus under 14-day hindlimb unloading of rats.

The results suggest that one of the possible mechanisms underlying pineal gland dysfunction during aging is the effect of lipid peroxidation on eEF-2, which is a key component of protein synthesis machinery.
Title: Aging and Oxidative Stress Decrease Pineal Elongation Factor 2: In Vivo Protective Effect of Melatonin in Young Rats Treated With Cumene Hydroperoxide.

Translational inhibition by CORM-2 is a result of eEF2 phosphorylation due to inhibition of PI3K-Akt-mTOR signaling.
Title: Carbon monoxide releasing molecule-2 CORM-2 represses global protein synthesis by inhibition of eukaryotic elongation factor eEF2.

melatonin prevented the molecular changes in eEF-2 and the decline in protein synthesis rate secondary to lipid peroxidation.
Title: In vitro and in vivo protection by melatonin against the decline of elongation factor-2 caused by lipid peroxidation: preservation of protein synthesis.

oxidative stress could be involved in the alterations of eEF-2, which forms adducts with malondialdehyde and 4-hydroxynonenal in the aging hypothalamo-hypophyseal system
Title: Effect of aging and oxidative stress on elongation factor-2 in hypothalamus and hypophysis.

The following Eef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03376	NM_017245.2 Latest version!	Rattus norvegicus eukaryotic translation elongation factor 2 (Eef2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eef2 ( NM_017245.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058941.1 Rattus norvegicus eukaryotic translation elongation factor 2 (Eef2), mRNA.

Price & Availability↑

CloneID	ORa03376
Clone ID Related Accession (Same CDS sequence)	NM_017245.2
Accession Version	NM_017245.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	elongation factor 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC066661.1. On Mar 14, 2004 this sequence version replaced NM_017245.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066661.1, Y07504.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTGAACT TCACAGTAGA TCAGATCCGT GCCATCATGG ACAAGAAAGC CAACATCCGG 
AACATGTCAG TCATCGCTCA CGTGGACCAC GGCAAGTCCA CGTTGACCGA CTCCCTTGTG 
TGCAAGGCTG GCATCATCGC CTCTGCCCGA GCCGGGGAGA CACGCTTCAC TGACACTCGA 
AAGGATGAGC AGGAGCGCTG CATCACCATC AAGTCCACTG CCATCTCCCT CTTCTATGAG 
CTCTCCGAGA ACGACCTGAA CTTCATTAAG CAGAGCAAGG ATGGCTCGGG CTTCCTCATC 
AACCTCATTG ACTCTCCAGG TCATGTGGAC TTCTCCTCAG AGGTGACCGC TGCTCTGCGT 
GTCACTGATG GAGCACTGGT GGTGGTGGAC TGTGTGTCTG GTGTCTGTGT GCAGACAGAG 
ACAGTGCTGC GGCAGGCCAT CGCTGAGCGC ATCAAGCCCG TGCTGATGAT GAACAAGATG 
GACCGGGCCC TGCTGGAACT GCAACTGGAG CCTGAGGAGC TCTACCAGAC CTTCCAGCGC 
ATCGTGGAGA ACGTCAATGT CATCATCTCC ACCTATGGCG AGGGCGAGAG TGGACCCATG 
GGCAATATCA TGATCGATCC TGTCCTGGGC ACTGTAGGCT TTGGCTCTGG CCTGCATGGG 
TGGGCCTTCA CACTGAAGCA GTTTGCAGAG ATGTATGTGG CCAAGTTTGC AGCCAAGGGT 
GAGGGCCAGC TGGGTGCAGC TGAGCGGGCC AAGAAGGTAG AAGACATGAT GAAGAAGCTG 
TGGGGAGACC GGTACTTTGA CCCGGCCAAC GGCAAGTTCA GCAAGTCAGC CAATAGCCCA 
GACGGGAAGA AACTTCCCCG CACCTTCTGC CAGCTCATCT TGGACCCCAT CTTCAAGGTG 
TTTGATGCCA TCATGAACTT CAGGAAGGAG GAGACAGCCA AGCTGATCGA GAAGCTGGAC 
ATCAAGCTGG ACAGTGAGGA CAAGGACAAG GAGGGCAAAC CACTGCTGAA GGCTGTGATG 
CGTCGCTGGC TGCCTGCTGG TGACGCCCTG CTACAGATGA TCACCATCCA CTTGCCGTCC 
CCGGTCACTG CACAGAAGTA CCGTTGTGAG CTGCTGTACG AGGGCCCACC TGATGACGAG 
GCCGCCATGG GTATTAAGAG CTGCGACCCC AAAGGCCCCC TAATGATGTA CATCTCCAAG 
ATGGTGCCAA CATCTGACAA AGGCCGCTTC TATGCCTTCG GTAGAGTGTT CTCTGGGGTG 
GTGTCCACAG GTCTGAAGGT CCGGATCATG GGCCCCAACT ATACACCTGG GAAGAAGGAG 
GACCTGTACC TGAAGCCTAT CCAGAGGACC ATTCTGATGA TGGGCCGCTA TGTGGAACCC 
ATTGAGGATG TGCCCTGTGG CAACATTGTG GGGTTGGTCG GGGTGGACCA GTTCCTTGTG 
AAGACCGGCA CCATCACTAC CTTTGAGCAC GCTCACAACA TGCGGGTGAT GAAGTTCAGC 
GTCAGCCCTG TCGTCAGGGT GGCAGTGGAG GCCAAGAACC CAGCTGACCT GCCCAAGCTG 
GTGGAGGGGC TGAAGCGGCT GGCTAAGTCT GACCCTATGG TGCAGTGCAT CATCGAGGAG 
TCTGGGGAGC ACATCATTGC TGGTGCTGGA GAGCTGCACC TGGAGATCTG CCTTAAGGAC 
CTGGAGGAGG ACCATGCCTG CATCCCCATC AAGAAATCTG ACCCTGTCGT GTCCTACCGG 
GAGACAGTCA GTGAGGAGTC CAACGTGCTC TGTCTTTCCA AGTCCCCAAA CAAGCACAAC 
AGGCTGTACA TGAAGGCCAG GCCCTTCCCT GATGGCCTAG CAGAGGACAT CGATAAGGGT 
GAGGTATCTG CCCGCCAGGA GCTCAAGGCG CGTGCCCGTT ACCTGGCCGA AAAGTATGAG 
TGGGATGTTG CTGAAGCCCG CAAGATCTGG TGCTTTGGAC CTGATGGCAC TGGTCCCAAC 
ATTCTCACCG ACATCACCAA GGGTGTGCAG TACCTGAATG AGATCAAGGA CAGTGTGGTG 
GCTGGATTCC AGTGGGCCAC TAAGGAGGGT GCTCTTTGTG AGGAGAACAT GCGTGGTGTG 
CGATTTGATG TCCATGATGT GACCCTGCAT GCTGATGCCA TCCACCGAGG AGGTGGTCAG 
ATCATCCCCA CAGCGCGCCG CTGCCTCTAT GCCAGTGTGC TGACTGCACA GCCCCGCCTC 
ATGGAGCCTA TCTACCTGGT GGAGATTCAG TGTCCTGAGC AAGTGGTGGG CGGAATCTAT 
GGTGTCCTGA ACAGGAAGCG TGGCCATGTC TTTGAGGAGT CCCAGGTAGC TGGGACCCCC 
ATGTTCGTGG TCAAGGCCTA CCTGCCTGTC AATGAATCCT TTGGCTTCAC TGCCGACCTT 
CGATCCAACA CTGGTGGCCA GGCCTTCCCC CAGTGTGTGT TTGACCACTG GCAGATCCTG 
CCTGGGGACC CCTTTGACAA CAGCAGCCGC CCCAGCCAAG TTGTAGCTGA GACCCGCAAG 
CGCAAAGGCC TGAAGGAGGG CATCCCAGCG CTGGACAACT TCCTGGACAA ACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058941.1
CDS	64..2640
Translation	MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGSGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLGAAERAKKVEDMMKKLWGDRYFDPANGKFSKSANSPDGKKLPRTFCQLILDPIFKVFDAIMNFRKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGVVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKARARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL

Target ORF information:

RefSeq Version	NM_017245.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic translation elongation factor 2 (Eef2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017245.2

ORF Insert Sequence:

ATGGTGAACT TCACAGTAGA TCAGATCCGT GCCATCATGG ACAAGAAAGC CAACATCCGG 
AACATGTCAG TCATCGCTCA CGTGGACCAC GGCAAGTCCA CGTTGACCGA CTCCCTTGTG 
TGCAAGGCTG GCATCATCGC CTCTGCCCGA GCCGGGGAGA CACGCTTCAC TGACACTCGA 
AAGGATGAGC AGGAGCGCTG CATCACCATC AAGTCCACTG CCATCTCCCT CTTCTATGAG 
CTCTCCGAGA ACGACCTGAA CTTCATTAAG CAGAGCAAGG ATGGCTCGGG CTTCCTCATC 
AACCTCATTG ACTCTCCAGG TCATGTGGAC TTCTCCTCAG AGGTGACCGC TGCTCTGCGT 
GTCACTGATG GAGCACTGGT GGTGGTGGAC TGTGTGTCTG GTGTCTGTGT GCAGACAGAG 
ACAGTGCTGC GGCAGGCCAT CGCTGAGCGC ATCAAGCCCG TGCTGATGAT GAACAAGATG 
GACCGGGCCC TGCTGGAACT GCAACTGGAG CCTGAGGAGC TCTACCAGAC CTTCCAGCGC 
ATCGTGGAGA ACGTCAATGT CATCATCTCC ACCTATGGCG AGGGCGAGAG TGGACCCATG 
GGCAATATCA TGATCGATCC TGTCCTGGGC ACTGTAGGCT TTGGCTCTGG CCTGCATGGG 
TGGGCCTTCA CACTGAAGCA GTTTGCAGAG ATGTATGTGG CCAAGTTTGC AGCCAAGGGT 
GAGGGCCAGC TGGGTGCAGC TGAGCGGGCC AAGAAGGTAG AAGACATGAT GAAGAAGCTG 
TGGGGAGACC GGTACTTTGA CCCGGCCAAC GGCAAGTTCA GCAAGTCAGC CAATAGCCCA 
GACGGGAAGA AACTTCCCCG CACCTTCTGC CAGCTCATCT TGGACCCCAT CTTCAAGGTG 
TTTGATGCCA TCATGAACTT CAGGAAGGAG GAGACAGCCA AGCTGATCGA GAAGCTGGAC 
ATCAAGCTGG ACAGTGAGGA CAAGGACAAG GAGGGCAAAC CACTGCTGAA GGCTGTGATG 
CGTCGCTGGC TGCCTGCTGG TGACGCCCTG CTACAGATGA TCACCATCCA CTTGCCGTCC 
CCGGTCACTG CACAGAAGTA CCGTTGTGAG CTGCTGTACG AGGGCCCACC TGATGACGAG 
GCCGCCATGG GTATTAAGAG CTGCGACCCC AAAGGCCCCC TAATGATGTA CATCTCCAAG 
ATGGTGCCAA CATCTGACAA AGGCCGCTTC TATGCCTTCG GTAGAGTGTT CTCTGGGGTG 
GTGTCCACAG GTCTGAAGGT CCGGATCATG GGCCCCAACT ATACACCTGG GAAGAAGGAG 
GACCTGTACC TGAAGCCTAT CCAGAGGACC ATTCTGATGA TGGGCCGCTA TGTGGAACCC 
ATTGAGGATG TGCCCTGTGG CAACATTGTG GGGTTGGTCG GGGTGGACCA GTTCCTTGTG 
AAGACCGGCA CCATCACTAC CTTTGAGCAC GCTCACAACA TGCGGGTGAT GAAGTTCAGC 
GTCAGCCCTG TCGTCAGGGT GGCAGTGGAG GCCAAGAACC CAGCTGACCT GCCCAAGCTG 
GTGGAGGGGC TGAAGCGGCT GGCTAAGTCT GACCCTATGG TGCAGTGCAT CATCGAGGAG 
TCTGGGGAGC ACATCATTGC TGGTGCTGGA GAGCTGCACC TGGAGATCTG CCTTAAGGAC 
CTGGAGGAGG ACCATGCCTG CATCCCCATC AAGAAATCTG ACCCTGTCGT GTCCTACCGG 
GAGACAGTCA GTGAGGAGTC CAACGTGCTC TGTCTTTCCA AGTCCCCAAA CAAGCACAAC 
AGGCTGTACA TGAAGGCCAG GCCCTTCCCT GATGGCCTAG CAGAGGACAT CGATAAGGGT 
GAGGTATCTG CCCGCCAGGA GCTCAAGGCG CGTGCCCGTT ACCTGGCCGA AAAGTATGAG 
TGGGATGTTG CTGAAGCCCG CAAGATCTGG TGCTTTGGAC CTGATGGCAC TGGTCCCAAC 
ATTCTCACCG ACATCACCAA GGGTGTGCAG TACCTGAATG AGATCAAGGA CAGTGTGGTG 
GCTGGATTCC AGTGGGCCAC TAAGGAGGGT GCTCTTTGTG AGGAGAACAT GCGTGGTGTG 
CGATTTGATG TCCATGATGT GACCCTGCAT GCTGATGCCA TCCACCGAGG AGGTGGTCAG 
ATCATCCCCA CAGCGCGCCG CTGCCTCTAT GCCAGTGTGC TGACTGCACA GCCCCGCCTC 
ATGGAGCCTA TCTACCTGGT GGAGATTCAG TGTCCTGAGC AAGTGGTGGG CGGAATCTAT 
GGTGTCCTGA ACAGGAAGCG TGGCCATGTC TTTGAGGAGT CCCAGGTAGC TGGGACCCCC 
ATGTTCGTGG TCAAGGCCTA CCTGCCTGTC AATGAATCCT TTGGCTTCAC TGCCGACCTT 
CGATCCAACA CTGGTGGCCA GGCCTTCCCC CAGTGTGTGT TTGACCACTG GCAGATCCTG 
CCTGGGGACC CCTTTGACAA CAGCAGCCGC CCCAGCCAAG TTGTAGCTGA GACCCGCAAG 
CGCAAAGGCC TGAAGGAGGG CATCCCAGCG CTGGACAACT TCCTGGACAA ACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Eukaryotic elongation factor 2 kinase activation in M. soleus under 14-day hindlimb unloading of rats.
Doklady. Biochemistry and biophysics474(1)165-167(2017 May)
Lomonosova YN,Belova SP,Mirzoev TM,Kozlovskaya IB,Shenkman BS

Aging and Oxidative Stress Decrease Pineal Elongation Factor 2: In Vivo Protective Effect of Melatonin in Young Rats Treated With Cumene Hydroperoxide.
Journal of cellular biochemistry118(1)182-190(2017 Jan)
Mu?oz MF,Arg?elles S,Cano M,Marotta F,Ayala A

The mechanics of ribosomal translocation.
Biochimie11480-9(2015 Jul)
Achenbach J,Nierhaus KH

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

Homodimerization of RBPMS2 through a new RRM-interaction motif is necessary to control smooth muscle plasticity.
Nucleic acids research42(15)10173-84(2014 Sep)
Sagnol S,Yang Y,Bessin Y,Allemand F,Hapkova I,Notarnicola C,Guichou JF,Faure S,Labesse G,de Santa Barbara P

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Eef2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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