eef2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	eef2b
Entrez Gene ID	326929
Full Name	eukaryotic translation elongation factor 2b
Synonyms	EEF2,eef2l,fe49h02,wu:fe49h02,zgc:63584
General protein information	Preferred Names eukaryotic translation elongation factor 2b Names eukaryotic translation elongation factor 2b EF-2 eukaryotic translation elongation factor 2, like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200458.2	NP_956752.2	eukaryotic translation elongation factor 2b

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC100614367	XP_003316060.2
	EEF2	XP_001118006.2
Drosophila melanogaster (fruit fly)	Ef2b	NP_525105.2
Mus musculus (house mouse)	Eef2	NP_031933.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009441	XP_307854.2
Danio rerio (zebrafish)	eef2b	NP_956752.2
Arabidopsis thaliana (thale cress)	LOS1	NP_849818.1
Oryza sativa (rice)	Os01g0742200	NP_001044211.1
Bos taurus (cattle)	EEF2	NP_001068589.1
Gallus gallus (chicken)	EEF2	NP_990699.1
Caenorhabditis elegans (roundworm)	eef-2	NP_001251010.1
Homo sapiens (human)	EEF2	NP_001952.1
Canis lupus familiaris (dog)	EEF2	XP_533949.3
Rattus norvegicus (Norway rat)	Eef2	NP_058941.1
Xenopus tropicalis (tropical clawed frog)	eef2.1	NP_989255.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Dose-dependent effects of gamma radiation on the early zebrafish development and gene expression.
Hurem S, Mart?n LM, Brede DA, Skjerve E, Nourizadeh-Lillabadi R, Lind OC, Christensen T, Berg V, Teien HC, Salbu B, Oughton DH, Alestr?m P, Lyche JL
PloS one12(6)e0179259(2017)

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine induced Parkinson's disease in zebrafish.
Sarath Babu N, Murthy ChL, Kakara S, Sharma R, Brahmendra Swamy CV, Idris MM
Proteomics16(9)1407-20(2016 05)

Analysis of protein expression in developmental toxicity induced by MeHg in zebrafish.
Cuello S, Xim?nez-Emb?n P, Ruppen I, Schonthaler HB, Ashman K, Madrid Y, Luque-Garcia JL, C?mara C
The Analyst137(22)5302-11(2012 Nov)

An integrated transcriptomic and proteomic approach characterizing estrogenic and metabolic effects of 17 alpha-ethinylestradiol in zebrafish (Danio rerio).
De Wit M, Keil D, van der Ven K, Vandamme S, Witters E, De Coen W
General and comparative endocrinology167(2)190-201(2010 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EF-2 is a developmentally regulated gene and might play important roles during the early development of zebrafish embryos.
Title: Cloning, expression and functional study of translation elongation factor 2 (EF-2) in zebrafish.

The following eef2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eef2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33304	NM_200458.2 Latest version!	Danio rerio eukaryotic translation elongation factor 2b (eef2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eef2b ( NM_200458.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956752.2 Danio rerio eukaryotic translation elongation factor 2b (eef2b), mRNA.

Price & Availability↑

CloneID	ODa33304
Clone ID Related Accession (Same CDS sequence)	NM_200458.2
Accession Version	NM_200458.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	eukaryotic translation elongation factor 2b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY391422.1. On Jan 29, 2004 this sequence version replaced NM_200458.1. ##Evidence-Data-START## Transcript exon combination :: AY391422.1, BC063965.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGTGAACT TCACAGTCGA CCAGATCCGT GCCATCATGG ACAAAAAGTC CAACATCCGT 
AACATGTCTG TGATTGCGCA CGTGGATCAC GGGAAATCTA CACTGACCGA CTCTCTGGTG 
TCCAAGGCTG GAATCATTGC TTCCGCTCGC GCCGGAGAGA CCCGCTTCAC TGACACACGC 
AAAGACGAGC AGGAGCGCTG CATTACCATC AAATCCACCG CCATCTCCAT GTACTACGAG 
CTGACGGAGA ATGACTTGGC CTTCATTAAG CAGTGTAAGG ATGGATCTGG TTTCCTCATT 
AACCTGATCG ACTCTCCTGG CCACGTGGAC TTTTCCTCCG AGGTCACAGC TGCTCTGCGT 
GTCACTGATG GAGCTCTGGT TGTGGTGGAT TGTGTCTCTG GTGTGTGTGT GCAGACCGAG 
ACAGTGCTGA GACAGGCCAT CGCCGAGCGC ATTAAGCCCG TGCTGATGAT GAACAAGATG 
GACCGAGCCC TGCTGGAGCT GCAGCTGGAG CCCGAGGAGC TGTACCAGAC CTTCCAGCGC 
ATCGTGGAGA ACGTCAACGT CATCATCTCC ACCTACGGAG AGGATGAGGG TGGACCCATG 
GGCAACATCA TGATTGATCC AGTGATTGGA ACTGTGGGTT TCGGATCTGG TCTGCACGGC 
TGGGCCTTCA CTCTGAAGCA GTTTGCCGAG ATGTACGTGG CTAAGTTTGC TTCTAAAGGT 
GAAGCCCAGC TGAGCCCAGC TGACCGCTGC AAGAAAGTGG AGGACATGAT GAAGAAACTC 
TGGGGTGACA GGTATTTTGA CCCAGCCGGC GGTAAATTCA CCAAGACCGC CAATGGCCCT 
GATGGAAAGA AATATCCCCG CACCTTCGCC CAGCTCATCC TGGACCCCAT CTTCAAGGTG 
TTCGATGCCA TCATGAACTT CAAGAAAGAG GAAACCGCCA AACTGATCGA GAAGCTGGAC 
ATCAAGCTGG ACACAGAGGA TAAGGATAAA GAGGGCAAAC CCCTGCTGAA GGCAGTGATG 
CGCCGCTGGC TGCCCGCCGG AGAGGCTCTG CTACAGATGA TCACCATCCA CCTGCCCTCA 
CCCGTCACCG CACAGAAGTA CCGCTGCGAG CTGCTCTATG AAGGACCTGG AGATGATGAG 
GCTGCCATGG GTATCAAGAA CTGTGACCCG AAGGGACCCC TAATGATGTA CATCTCAAAA 
ATGGTGCCGA CCACTGATAA GGGTCGTTTC TACGCTTTCG GCCGTGTGTT CTCCGGCGTC 
GTGTCCACTG GTCTGAAAGT GCGAATCATG GGACCAAACT ACACCCCTGG AAAGAAAGAG 
GACCTTTACC TGAAGCCAAT TCAGAGGACC ATTCTGATGA TGGGACGTTA TGTGGAGCCC 
ATTGAAGACG TGCCCTGCGG AAACATCGTA GGATTGGTTG GCGTGGACCA GTTTTTGGTG 
AAGACCGGGA CCATCACCAC CTTCGATCAG GCTCACAACA TGCGTGTGAT GAAGTTCAGC 
GTCAGCCCTG TGGTCAGAGT CGCTGTGGAG GCCAAAAACC CTGCTGACCT GCCCAAACTC 
GTGGAGGGAC TGAAGCGTCT GGCCAAATCC GACCCTATGG TGCAGTGCAT CATCGAGGAG 
TCTGGAGAAC ACATCATCGC CGGCGCTGGA GAACTCCACC TGGAAATCTG CCTTAAAGAT 
CTGGAGGAGG ACCATGCCTG CATTCCACTC AAGAAATCAG ACCCGGTTGT GTCCTACAGG 
GAGACAGTCA GCGCAGAATC CGACCAGATG TGTTTGTCCA AATCCCCCAA TAAGCACAAC 
CGTCTGTACA TGAAGGCCCG GCCGTTCCCC GACGGTCTCG CTGAGGACAT CGATAAAGGC 
GACGTCTCGT CCCGGCAGGA GCTGAAGACC CGTGCTCGAT ACCTGGCCGA CAAATATGAG 
TGGGAGGTCA CCGAGGCCCG AAAGATCTGG TGCTTCGGCC CTGACGGCAC TGGCCCCAAT 
ATGCTGGTGG ACGTGACCAA AGGAGTGCAG TACCTGAACG AGATCAAGGA CAGCGTCGTG 
GCTGGATTCC AGTGGGCCAC AAAAGAGGGA GCTCTGTGTG AGGAGAACAT GCGTGCCGTT 
CGTTTCGACA TTCATGACGT GACTCTGCAC ACGGACGCCA TCCATCGTGG AGGCGGTCAG 
ATCATTCCCA CCGCCCGCAG AGTGCTGTAC GCCTGCCAGC TCACAGCCGA GCCCAGACTC 
ATGGAGCCCA TTTACCTGGT GGAGATCCAG TGCCCAGAAC AGGTTGTCGG TGGCATCTAC 
GGTGTGTTGA ACAGAAAACG AGGACACGTG TTTGAGGAGT CTCAGGTCAT GGGAACACCC 
ATGTTTGTCG TAAAGGCTTA CCTGCCTGTC AACGAGTCTT TCGGTTTCAC AGCAGATCTG 
CGCTCCAACA CCGGCGGTCA GGCCTTCCCA CAGTGTGTGT TTGACCACTG GCAGATCCTG 
CCCGGAGACC CCAAAGACGC AGCCTCCAAA CCCTGCCAGA TCGTGGCGGA CACTCGCAAA 
CGCAAGGGCC TGAAGGAGGG AATCCCAGCA CTCGACAACT TCCTGGACAA GTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956752.2
CDS	55..2631
Translation	MVNFTVDQIRAIMDKKSNIRNMSVIAHVDHGKSTLTDSLVSKAGIIASARAGETRFTDTRKDEQERCITIKSTAISMYYELTENDLAFIKQCKDGSGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEDEGGPMGNIMIDPVIGTVGFGSGLHGWAFTLKQFAEMYVAKFASKGEAQLSPADRCKKVEDMMKKLWGDRYFDPAGGKFTKTANGPDGKKYPRTFAQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDTEDKDKEGKPLLKAVMRRWLPAGEALLQMITIHLPSPVTAQKYRCELLYEGPGDDEAAMGIKNCDPKGPLMMYISKMVPTTDKGRFYAFGRVFSGVVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFDQAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPLKKSDPVVSYRETVSAESDQMCLSKSPNKHNRLYMKARPFPDGLAEDIDKGDVSSRQELKTRARYLADKYEWEVTEARKIWCFGPDGTGPNMLVDVTKGVQYLNEIKDSVVAGFQWATKEGALCEENMRAVRFDIHDVTLHTDAIHRGGGQIIPTARRVLYACQLTAEPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVMGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPKDAASKPCQIVADTRKRKGLKEGIPALDNFLDKL

Target ORF information:

RefSeq Version	NM_200458.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eukaryotic translation elongation factor 2b (eef2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200458.2

ORF Insert Sequence:

ATGGTGAACT TCACAGTCGA CCAGATCCGT GCCATCATGG ACAAAAAGTC CAACATCCGT 
AACATGTCTG TGATTGCGCA CGTGGATCAC GGGAAATCTA CACTGACCGA CTCTCTGGTG 
TCCAAGGCTG GAATCATTGC TTCCGCTCGC GCCGGAGAGA CCCGCTTCAC TGACACACGC 
AAAGACGAGC AGGAGCGCTG CATTACCATC AAATCCACCG CCATCTCCAT GTACTACGAG 
CTGACGGAGA ATGACTTGGC CTTCATTAAG CAGTGTAAGG ATGGATCTGG TTTCCTCATT 
AACCTGATCG ACTCTCCTGG CCACGTGGAC TTTTCCTCCG AGGTCACAGC TGCTCTGCGT 
GTCACTGATG GAGCTCTGGT TGTGGTGGAT TGTGTCTCTG GTGTGTGTGT GCAGACCGAG 
ACAGTGCTGA GACAGGCCAT CGCCGAGCGC ATTAAGCCCG TGCTGATGAT GAACAAGATG 
GACCGAGCCC TGCTGGAGCT GCAGCTGGAG CCCGAGGAGC TGTACCAGAC CTTCCAGCGC 
ATCGTGGAGA ACGTCAACGT CATCATCTCC ACCTACGGAG AGGATGAGGG TGGACCCATG 
GGCAACATCA TGATTGATCC AGTGATTGGA ACTGTGGGTT TCGGATCTGG TCTGCACGGC 
TGGGCCTTCA CTCTGAAGCA GTTTGCCGAG ATGTACGTGG CTAAGTTTGC TTCTAAAGGT 
GAAGCCCAGC TGAGCCCAGC TGACCGCTGC AAGAAAGTGG AGGACATGAT GAAGAAACTC 
TGGGGTGACA GGTATTTTGA CCCAGCCGGC GGTAAATTCA CCAAGACCGC CAATGGCCCT 
GATGGAAAGA AATATCCCCG CACCTTCGCC CAGCTCATCC TGGACCCCAT CTTCAAGGTG 
TTCGATGCCA TCATGAACTT CAAGAAAGAG GAAACCGCCA AACTGATCGA GAAGCTGGAC 
ATCAAGCTGG ACACAGAGGA TAAGGATAAA GAGGGCAAAC CCCTGCTGAA GGCAGTGATG 
CGCCGCTGGC TGCCCGCCGG AGAGGCTCTG CTACAGATGA TCACCATCCA CCTGCCCTCA 
CCCGTCACCG CACAGAAGTA CCGCTGCGAG CTGCTCTATG AAGGACCTGG AGATGATGAG 
GCTGCCATGG GTATCAAGAA CTGTGACCCG AAGGGACCCC TAATGATGTA CATCTCAAAA 
ATGGTGCCGA CCACTGATAA GGGTCGTTTC TACGCTTTCG GCCGTGTGTT CTCCGGCGTC 
GTGTCCACTG GTCTGAAAGT GCGAATCATG GGACCAAACT ACACCCCTGG AAAGAAAGAG 
GACCTTTACC TGAAGCCAAT TCAGAGGACC ATTCTGATGA TGGGACGTTA TGTGGAGCCC 
ATTGAAGACG TGCCCTGCGG AAACATCGTA GGATTGGTTG GCGTGGACCA GTTTTTGGTG 
AAGACCGGGA CCATCACCAC CTTCGATCAG GCTCACAACA TGCGTGTGAT GAAGTTCAGC 
GTCAGCCCTG TGGTCAGAGT CGCTGTGGAG GCCAAAAACC CTGCTGACCT GCCCAAACTC 
GTGGAGGGAC TGAAGCGTCT GGCCAAATCC GACCCTATGG TGCAGTGCAT CATCGAGGAG 
TCTGGAGAAC ACATCATCGC CGGCGCTGGA GAACTCCACC TGGAAATCTG CCTTAAAGAT 
CTGGAGGAGG ACCATGCCTG CATTCCACTC AAGAAATCAG ACCCGGTTGT GTCCTACAGG 
GAGACAGTCA GCGCAGAATC CGACCAGATG TGTTTGTCCA AATCCCCCAA TAAGCACAAC 
CGTCTGTACA TGAAGGCCCG GCCGTTCCCC GACGGTCTCG CTGAGGACAT CGATAAAGGC 
GACGTCTCGT CCCGGCAGGA GCTGAAGACC CGTGCTCGAT ACCTGGCCGA CAAATATGAG 
TGGGAGGTCA CCGAGGCCCG AAAGATCTGG TGCTTCGGCC CTGACGGCAC TGGCCCCAAT 
ATGCTGGTGG ACGTGACCAA AGGAGTGCAG TACCTGAACG AGATCAAGGA CAGCGTCGTG 
GCTGGATTCC AGTGGGCCAC AAAAGAGGGA GCTCTGTGTG AGGAGAACAT GCGTGCCGTT 
CGTTTCGACA TTCATGACGT GACTCTGCAC ACGGACGCCA TCCATCGTGG AGGCGGTCAG 
ATCATTCCCA CCGCCCGCAG AGTGCTGTAC GCCTGCCAGC TCACAGCCGA GCCCAGACTC 
ATGGAGCCCA TTTACCTGGT GGAGATCCAG TGCCCAGAAC AGGTTGTCGG TGGCATCTAC 
GGTGTGTTGA ACAGAAAACG AGGACACGTG TTTGAGGAGT CTCAGGTCAT GGGAACACCC 
ATGTTTGTCG TAAAGGCTTA CCTGCCTGTC AACGAGTCTT TCGGTTTCAC AGCAGATCTG 
CGCTCCAACA CCGGCGGTCA GGCCTTCCCA CAGTGTGTGT TTGACCACTG GCAGATCCTG 
CCCGGAGACC CCAAAGACGC AGCCTCCAAA CCCTGCCAGA TCGTGGCGGA CACTCGCAAA 
CGCAAGGGCC TGAAGGAGGG AATCCCAGCA CTCGACAACT TCCTGGACAA GTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Dose-dependent effects of gamma radiation on the early zebrafish development and gene expression.
PloS one12(6)e0179259(2017)
Hurem S,Mart?n LM,Brede DA,Skjerve E,Nourizadeh-Lillabadi R,Lind OC,Christensen T,Berg V,Teien HC,Salbu B,Oughton DH,Alestr?m P,Lyche JL

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine induced Parkinson's disease in zebrafish.
Proteomics16(9)1407-20(2016 05)
Sarath Babu N,Murthy ChL,Kakara S,Sharma R,Brahmendra Swamy CV,Idris MM

Analysis of protein expression in developmental toxicity induced by MeHg in zebrafish.
The Analyst137(22)5302-11(2012 Nov)
Cuello S,Xim?nez-Emb?n P,Ruppen I,Schonthaler HB,Ashman K,Madrid Y,Luque-Garcia JL,C?mara C

An integrated transcriptomic and proteomic approach characterizing estrogenic and metabolic effects of 17 alpha-ethinylestradiol in zebrafish (Danio rerio).
General and comparative endocrinology167(2)190-201(2010 Jun)
De Wit M,Keil D,van der Ven K,Vandamme S,Witters E,De Coen W

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eef2b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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