Nepl18 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nepl18
Entrez Gene ID	43410
Full Name	CG14528 gene product from transcript CG14528-RA
Synonyms	Dmel\CG14528
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98F1-98F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_143386.3	NP_651643.1	Neprilysin-like 18

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG14527	NP_651645.1
Drosophila melanogaster (fruit fly)	CG14526	NP_651646.2
Drosophila melanogaster (fruit fly)	CG4721	NP_651098.2
Drosophila melanogaster (fruit fly)	CG14523	NP_651644.1
Drosophila melanogaster (fruit fly)	CG14529	NP_651641.1
Drosophila melanogaster (fruit fly)	CG14528	NP_651643.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nepl18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nepl18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06424	NM_143386.3 Latest version!	Drosophila melanogaster Neprilysin-like 18 (Nepl18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nepl18 ( NM_143386.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651643.1 Drosophila melanogaster Neprilysin-like 18 (Nepl18), mRNA.

Price & Availability↑

CloneID	OFa06424
Clone ID Related Accession (Same CDS sequence)	NM_143386.3
Accession Version	NM_143386.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Neprilysin-like 18
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143386.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGACGA GCCGGGTGTT GTGGCTCTGC CTTTTGGTGG CCATAGTTCC AGCTATGGCC 
AAGCCATCGG ATGCTGGTGA TCCCTTTGCC GACGACTTGA CTTCGGATTA TGCGAAGAAA 
ATCATTGTGC AAGCGAAAGT GGCCGAAATG AAGACAATGA TGAAGCCGGA GGTATCGCCC 
TGCGACGACT TCTACACTCA TGCCTGTGGA AACTGGCATC GCCACAATCC TGCCCAGCTA 
TTGGGCGACG TTATGACCGA CAACTTCAAA TTAATATCCA AGGGATTCGA TCGACGGCTC 
CAGCGGTTGC TGCGCGCCAA TGACCTGCAA TCCGAGCTGG AAAAGAAGAT GCAGCGTTTC 
TACATGTCCT GCAATCTGGT CCATCGCGAT GATGTGCACT ACAAGCTGGC CCTAGAGAAC 
GTCATCCGGG AGTACGGAAC ACTCCCGGCC TTGGTTGGGG CCCAGTGGAA CTCCTCCGAC 
TTCAGTTGGT GGCGAACAGT GGCCCAGATA CAGCACAAAT ACGGAAAACA GATCATTATA 
GGCCTGGAGA TAATGCACGA TATCCGGAAC ACATCGGTGA ACCGCGTGTA CATAAGTCAG 
CCAGATTTTA AGACAATTAA CACTCGCCTA ACAAATCTCA TGGAGGAGGT CCGCACATCC 
ATGGATCTGC AGCAATATTT CGGTCTGAGT TCCAGCGTTG CAAAACACAC AGCCGAGCAG 
CTCACTGAGT TGGAGAGAAC TTTCTCATCC GGTGGCTCTG GTGCAGCGAC TCTGCAGGAA 
TCATTGTCCC TCTATACGGT GGCCGATCTT CAAGAGAAAT ACAGCGACCA TCTGAATTTC 
ACCGAATTCC TGGGCCTGAT CCTCGGCGAG GAGAACATAC CGAAGTCGTT GTACATCTAT 
GACGAGGAGT ACCTGGACAA AGCACTTCTG ACCATGCGAA GCACACCACT GGCCACACAG 
GCAAACTACG TTTTGTGGAA GCTGCTCGAG GACTTTCTGG TGGACGCCCA GCCCAAGGAT 
ATGGTTAAAT GGTGCACCGA ATCCACTAAG AAGTACTTCG GAAAACTCGC CGAGCACGCA 
GTTTACAAGC GATATCGCAA CCCGGACGTG GAGTCGGAAG TTCACAAGAT CTGGGATCAG 
ATCAAAGGAA TCTTTAGGCA ACGTCTGTTG GGGGACAAAC TGGACTGGAT ATCCAATGCC 
ACCAGGACGT TGGCCATCGA AAAGCTAGAA CGCATGCATC TGAACATCAA CTCCTACGAC 
GAGGAGAACT TTGAGAATGT ATATGGAGAG GTGTTCATCG ATCGCCTCAA TTACGTTTCA 
AATGTTCAGC AGCTGCTCAT TGCCAAGGGC ATCAGATTTG TGGCAAGGAT TAATCAGCCG 
GCAGACTCCG TAGATGCCAC AGAGCTACTC GGGTTTACTC CCGCCTACAA CATTCAGGAG 
AACAACATCA CGATCCCGGT GGCCCTGCTG CAGCCACGCT ATTTCTGGGG TGACCAGTAT 
CCGGAGGCGT TGAAGTACGC CACTCTAGGC TATCTCCTCG CCCACGAGAT GCTACACGGC 
TTCGACGATG ATGGAAGGCA GTACGATGCG TCCGGTAACC TGGCGCCTTG GTGGGACTTG 
AAATCCCGCT ACGAGTTTGA GGAACGGCGC AAGTGCTTCC AAGCCCAGTA TCACGAGTAC 
CAATACGGTG GATCAAAGCT GCCGGAGAGC AAGGATCAGT CAGAGAACAT AGCGGATAGT 
GGAGGCCTTA AATTGGCCTA CGCCGCCTAC GAATTGTGGC TGGGTCAGCA ATCCGAGGAG 
GTGCTTCAAC GTGAAACCAT GGAGGGTCTG CCCTTCAACA GTCGCCAGCT TTTCTTCTTG 
GGATACGCAC AATTGTTGTG CGACGACGTT CAGTTTCTCT TCCAGCCCTG GGTGAGTCAA 
TCGGACAGGC ATGCACCCAG CAAGTACCGG GTGATTGGAC CGCTGTCCAA TTTTCAGGAG 
TTCCCTTGGG TCTTCAACTG CTCGCAATCG GCGCCCATGG ATCCGGAGTA CAAGTGCGCC 
ATCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651643.1
CDS	26..2074
Translation	MMTSRVLWLCLLVAIVPAMAKPSDAGDPFADDLTSDYAKKIIVQAKVAEMKTMMKPEVSPCDDFYTHACGNWHRHNPAQLLGDVMTDNFKLISKGFDRRLQRLLRANDLQSELEKKMQRFYMSCNLVHRDDVHYKLALENVIREYGTLPALVGAQWNSSDFSWWRTVAQIQHKYGKQIIIGLEIMHDIRNTSVNRVYISQPDFKTINTRLTNLMEEVRTSMDLQQYFGLSSSVAKHTAEQLTELERTFSSGGSGAATLQESLSLYTVADLQEKYSDHLNFTEFLGLILGEENIPKSLYIYDEEYLDKALLTMRSTPLATQANYVLWKLLEDFLVDAQPKDMVKWCTESTKKYFGKLAEHAVYKRYRNPDVESEVHKIWDQIKGIFRQRLLGDKLDWISNATRTLAIEKLERMHLNINSYDEENFENVYGEVFIDRLNYVSNVQQLLIAKGIRFVARINQPADSVDATELLGFTPAYNIQENNITIPVALLQPRYFWGDQYPEALKYATLGYLLAHEMLHGFDDDGRQYDASGNLAPWWDLKSRYEFEERRKCFQAQYHEYQYGGSKLPESKDQSENIADSGGLKLAYAAYELWLGQQSEEVLQRETMEGLPFNSRQLFFLGYAQLLCDDVQFLFQPWVSQSDRHAPSKYRVIGPLSNFQEFPWVFNCSQSAPMDPEYKCAIY

Target ORF information:

RefSeq Version	NM_143386.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Neprilysin-like 18 (Nepl18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143386.3

ORF Insert Sequence:

ATGATGACGA GCCGGGTGTT GTGGCTCTGC CTTTTGGTGG CCATAGTTCC AGCTATGGCC 
AAGCCATCGG ATGCTGGTGA TCCCTTTGCC GACGACTTGA CTTCGGATTA TGCGAAGAAA 
ATCATTGTGC AAGCGAAAGT GGCCGAAATG AAGACAATGA TGAAGCCGGA GGTATCGCCC 
TGCGACGACT TCTACACTCA TGCCTGTGGA AACTGGCATC GCCACAATCC TGCCCAGCTA 
TTGGGCGACG TTATGACCGA CAACTTCAAA TTAATATCCA AGGGATTCGA TCGACGGCTC 
CAGCGGTTGC TGCGCGCCAA TGACCTGCAA TCCGAGCTGG AAAAGAAGAT GCAGCGTTTC 
TACATGTCCT GCAATCTGGT CCATCGCGAT GATGTGCACT ACAAGCTGGC CCTAGAGAAC 
GTCATCCGGG AGTACGGAAC ACTCCCGGCC TTGGTTGGGG CCCAGTGGAA CTCCTCCGAC 
TTCAGTTGGT GGCGAACAGT GGCCCAGATA CAGCACAAAT ACGGAAAACA GATCATTATA 
GGCCTGGAGA TAATGCACGA TATCCGGAAC ACATCGGTGA ACCGCGTGTA CATAAGTCAG 
CCAGATTTTA AGACAATTAA CACTCGCCTA ACAAATCTCA TGGAGGAGGT CCGCACATCC 
ATGGATCTGC AGCAATATTT CGGTCTGAGT TCCAGCGTTG CAAAACACAC AGCCGAGCAG 
CTCACTGAGT TGGAGAGAAC TTTCTCATCC GGTGGCTCTG GTGCAGCGAC TCTGCAGGAA 
TCATTGTCCC TCTATACGGT GGCCGATCTT CAAGAGAAAT ACAGCGACCA TCTGAATTTC 
ACCGAATTCC TGGGCCTGAT CCTCGGCGAG GAGAACATAC CGAAGTCGTT GTACATCTAT 
GACGAGGAGT ACCTGGACAA AGCACTTCTG ACCATGCGAA GCACACCACT GGCCACACAG 
GCAAACTACG TTTTGTGGAA GCTGCTCGAG GACTTTCTGG TGGACGCCCA GCCCAAGGAT 
ATGGTTAAAT GGTGCACCGA ATCCACTAAG AAGTACTTCG GAAAACTCGC CGAGCACGCA 
GTTTACAAGC GATATCGCAA CCCGGACGTG GAGTCGGAAG TTCACAAGAT CTGGGATCAG 
ATCAAAGGAA TCTTTAGGCA ACGTCTGTTG GGGGACAAAC TGGACTGGAT ATCCAATGCC 
ACCAGGACGT TGGCCATCGA AAAGCTAGAA CGCATGCATC TGAACATCAA CTCCTACGAC 
GAGGAGAACT TTGAGAATGT ATATGGAGAG GTGTTCATCG ATCGCCTCAA TTACGTTTCA 
AATGTTCAGC AGCTGCTCAT TGCCAAGGGC ATCAGATTTG TGGCAAGGAT TAATCAGCCG 
GCAGACTCCG TAGATGCCAC AGAGCTACTC GGGTTTACTC CCGCCTACAA CATTCAGGAG 
AACAACATCA CGATCCCGGT GGCCCTGCTG CAGCCACGCT ATTTCTGGGG TGACCAGTAT 
CCGGAGGCGT TGAAGTACGC CACTCTAGGC TATCTCCTCG CCCACGAGAT GCTACACGGC 
TTCGACGATG ATGGAAGGCA GTACGATGCG TCCGGTAACC TGGCGCCTTG GTGGGACTTG 
AAATCCCGCT ACGAGTTTGA GGAACGGCGC AAGTGCTTCC AAGCCCAGTA TCACGAGTAC 
CAATACGGTG GATCAAAGCT GCCGGAGAGC AAGGATCAGT CAGAGAACAT AGCGGATAGT 
GGAGGCCTTA AATTGGCCTA CGCCGCCTAC GAATTGTGGC TGGGTCAGCA ATCCGAGGAG 
GTGCTTCAAC GTGAAACCAT GGAGGGTCTG CCCTTCAACA GTCGCCAGCT TTTCTTCTTG 
GGATACGCAC AATTGTTGTG CGACGACGTT CAGTTTCTCT TCCAGCCCTG GGTGAGTCAA 
TCGGACAGGC ATGCACCCAG CAAGTACCGG GTGATTGGAC CGCTGTCCAA TTTTCAGGAG 
TTCCCTTGGG TCTTCAACTG CTCGCAATCG GCGCCCATGG ATCCGGAGTA CAAGTGCGCC 
ATCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nepl18 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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