Nepl21 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nepl21
Entrez Gene ID	43413
Full Name	CG14526 gene product from transcript CG14526-RC
Synonyms	BcDNA:GH07188,Dmel\CG14526
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98F1-98F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_143389.3	NP_651646.2	Neprilysin-like 21

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG14527	NP_651645.1
Drosophila melanogaster (fruit fly)	CG14526	NP_651646.2
Drosophila melanogaster (fruit fly)	CG4721	NP_651098.2
Drosophila melanogaster (fruit fly)	CG14523	NP_651644.1
Drosophila melanogaster (fruit fly)	CG14529	NP_651641.1
Drosophila melanogaster (fruit fly)	CG14528	NP_651643.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nepl21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nepl21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06427	NM_143389.3 Latest version!	Drosophila melanogaster Neprilysin-like 21 (Nepl21), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nepl21 ( NM_143389.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651646.2 Drosophila melanogaster Neprilysin-like 21 (Nepl21), partial mRNA.

Price & Availability↑

CloneID	OFa06427
Clone ID Related Accession (Same CDS sequence)	NM_143389.3
Accession Version	NM_143389.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Neprilysin-like 21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_143389.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGGGAATTA AAGCGAAGCA CAACGCGAAG CAGTTGCTCT GCATTTGCAT TGCTGGCCAC 
GTCCTAAGTG CGATGGTCGC GGCTGCTCCT ACCAGCGAGG AAGATCTCAA ACAGGACACG 
CCCTACATGA AGGAACTATT GCGTCAGGCG AAGACTGCTG AGATAGAGAG CTTTATGGAC 
CAGAAGGCGG ATCCGTGCAA CGATTTCTAC GCATTCTCCT GTGGCAATTA TAAGCGCATC 
AACTCGGCTT TGAGCATGCA GGTGGTCACC ACTGGAGTAT TCGAGACTCT GACCAAAGGA 
CTTAACCGAA AGATCCTGAA GATGCTCAAC ACACCCCACG ATTCCCACGA CACTCCGGAA 
GACATTAAGG TGAAGCATTT CTACGAGTCC TGTCTCCAGA TCAAGGAGCT CAACTCGACG 
TACAGCGAAA AGCTGAAGCG GCTCATTGCA GAATTTGGAA CGATGCCGCT GCTCGAAGGA 
TCCTCCTGGC AGGAGGACGA CTTCGACTGG CTGAATACTA CTGCTCGCAT GGCCTACCGA 
TACGGTATAA CTCCCATTTT CGGCATTGAG GTGAACAAAG ACTTGGCCAG CAATACCCGG 
AATCGGATAT ACTTGGGTCA GCAGGACTTC CCGCTGGAGG CGCGGTCCAT GTACGTCGAT 
GATGCAACAG CAGTATATCG TCAGAAATAT CGTAATAATA TCCAGCGCAT CTTGCAGCGT 
TACCTGGGCG TGAAGATGGA TTTGGCCAAA AAAACGGCCA AGGAACTGAT TGACTTTGAG 
GTGGACTTGG CTCAAGGACT GGTGGACGAG AGTGAAGGAC TAGATGTGGG TGAATTGACC 
CAGTTACTCA CAGTGGACGA AATCCAGAGA AGGTACTCCC CCACCTTGGA TATCGACCGA 
TTGCTATTTG TGAGCATGGG AGAGCGCATT TCAGACCAGA TCTACGAATA TAACAATCGT 
TATCAGCAAA ACTTGGTCGA AGTCATCAAG CGTACGCCGA AGCGCACTGT AGCCAACTAT 
ATATTTTTCC GGCTGATCTG GGAGTTTGTG GAGACGCCTT CAGATTCGCC GGAGAAGCAG 
ATGAAGGCAT GCGTGGACCT TACCAAAAAG TACTTTGCCA AGAATCTTGA CAACATGTTC 
TATCGTCGCT ACAACAACGA GAAATCCTCT AGAGAAATCG ACAACATGTG GCGTCAATTG 
AAGTCTACCT TTAACGAGAC CCTCCGTTCC AGTCCCGCCT TGAATTGGAT TGAGCGACCC 
ACCCGGAATC TTGCTATGGC GAAGCTACAG GCAATGACGC TGGAGGTCAA CAACTACGCA 
GACGACAACT TCACCGAGGA GTTTGCCGAA CTCAATCTGC AAAGTGACGA CTACGTGGAG 
AATGTGCGAT ATACAAGCCT ACTTGGGGCC AAACAGATGC GGGAGATGCT TCACAAGCCG 
GCCAAACCCT TTGAGGCGGG CTCCCAACTC AGTTACACCC CGGCCAACAT CCTGATAGAG 
AACACCATCA AGGTTCCGGT GGCCCTGCTA CAACCCTTCT ACATTTGGTC CGATGTCTAC 
CCCAATGCGA TTATGTTCGG AACCCTGGCC TCCCTAATTG GACACGAGTT AATCCACGGA 
TTCGACGACA GCGGGCGGAA GTTCGATGAG AAGGGAAACT CGAAGGATTG GTGGGATGAG 
AAGTCCAGCA GCAACTTCCT GAAACGAAGG GACTGCTTCA CCAAGCAGTA TGGCAGGTAT 
GTCTACGATG GCATTCAGTT AAAGGAGTCA ACCGCGCAGT CGGAGAACAT TGCGGACAAC 
GGCGGAATGC GACTGGCCTA CACCGCCTAC CGGAAATGGT ACGAAAACCA GTTGACACTT 
CCCAATGGGG CTCAGGATAT GACCAAGGAG ACCCTGCCCA ACCTGCGATA CACCGCCAAA 
CAACTATTCT TCATTAGCTT TGCTCAGAGC TGGTGCAATG ATGCACATCC CAGTGTGAAG 
GCACTTCAGG TGTCAACCGA CCAGCACATG CCCGGAAGAT TCCGCGTTAT TGGATCTTTA 
TCTAACTTCG AGGAGTTCTC GAAGGAGTTC AACTGCCCAG CCGGATCCGC CATGAATCCT 
AGTGAGAAAT GCATACTTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651646.2
CDS	1..2124
Translation	MGIKAKHNAKQLLCICIAGHVLSAMVAAAPTSEEDLKQDTPYMKELLRQAKTAEIESFMDQKADPCNDFYAFSCGNYKRINSALSMQVVTTGVFETLTKGLNRKILKMLNTPHDSHDTPEDIKVKHFYESCLQIKELNSTYSEKLKRLIAEFGTMPLLEGSSWQEDDFDWLNTTARMAYRYGITPIFGIEVNKDLASNTRNRIYLGQQDFPLEARSMYVDDATAVYRQKYRNNIQRILQRYLGVKMDLAKKTAKELIDFEVDLAQGLVDESEGLDVGELTQLLTVDEIQRRYSPTLDIDRLLFVSMGERISDQIYEYNNRYQQNLVEVIKRTPKRTVANYIFFRLIWEFVETPSDSPEKQMKACVDLTKKYFAKNLDNMFYRRYNNEKSSREIDNMWRQLKSTFNETLRSSPALNWIERPTRNLAMAKLQAMTLEVNNYADDNFTEEFAELNLQSDDYVENVRYTSLLGAKQMREMLHKPAKPFEAGSQLSYTPANILIENTIKVPVALLQPFYIWSDVYPNAIMFGTLASLIGHELIHGFDDSGRKFDEKGNSKDWWDEKSSSNFLKRRDCFTKQYGRYVYDGIQLKESTAQSENIADNGGMRLAYTAYRKWYENQLTLPNGAQDMTKETLPNLRYTAKQLFFISFAQSWCNDAHPSVKALQVSTDQHMPGRFRVIGSLSNFEEFSKEFNCPAGSAMNPSEKCILY

Target ORF information:

RefSeq Version	NM_143389.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Neprilysin-like 21 (Nepl21), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143389.3

ORF Insert Sequence:

ATGGGAATTA AAGCGAAGCA CAACGCGAAG CAGTTGCTCT GCATTTGCAT TGCTGGCCAC 
GTCCTAAGTG CGATGGTCGC GGCTGCTCCT ACCAGCGAGG AAGATCTCAA ACAGGACACG 
CCCTACATGA AGGAACTATT GCGTCAGGCG AAGACTGCTG AGATAGAGAG CTTTATGGAC 
CAGAAGGCGG ATCCGTGCAA CGATTTCTAC GCATTCTCCT GTGGCAATTA TAAGCGCATC 
AACTCGGCTT TGAGCATGCA GGTGGTCACC ACTGGAGTAT TCGAGACTCT GACCAAAGGA 
CTTAACCGAA AGATCCTGAA GATGCTCAAC ACACCCCACG ATTCCCACGA CACTCCGGAA 
GACATTAAGG TGAAGCATTT CTACGAGTCC TGTCTCCAGA TCAAGGAGCT CAACTCGACG 
TACAGCGAAA AGCTGAAGCG GCTCATTGCA GAATTTGGAA CGATGCCGCT GCTCGAAGGA 
TCCTCCTGGC AGGAGGACGA CTTCGACTGG CTGAATACTA CTGCTCGCAT GGCCTACCGA 
TACGGTATAA CTCCCATTTT CGGCATTGAG GTGAACAAAG ACTTGGCCAG CAATACCCGG 
AATCGGATAT ACTTGGGTCA GCAGGACTTC CCGCTGGAGG CGCGGTCCAT GTACGTCGAT 
GATGCAACAG CAGTATATCG TCAGAAATAT CGTAATAATA TCCAGCGCAT CTTGCAGCGT 
TACCTGGGCG TGAAGATGGA TTTGGCCAAA AAAACGGCCA AGGAACTGAT TGACTTTGAG 
GTGGACTTGG CTCAAGGACT GGTGGACGAG AGTGAAGGAC TAGATGTGGG TGAATTGACC 
CAGTTACTCA CAGTGGACGA AATCCAGAGA AGGTACTCCC CCACCTTGGA TATCGACCGA 
TTGCTATTTG TGAGCATGGG AGAGCGCATT TCAGACCAGA TCTACGAATA TAACAATCGT 
TATCAGCAAA ACTTGGTCGA AGTCATCAAG CGTACGCCGA AGCGCACTGT AGCCAACTAT 
ATATTTTTCC GGCTGATCTG GGAGTTTGTG GAGACGCCTT CAGATTCGCC GGAGAAGCAG 
ATGAAGGCAT GCGTGGACCT TACCAAAAAG TACTTTGCCA AGAATCTTGA CAACATGTTC 
TATCGTCGCT ACAACAACGA GAAATCCTCT AGAGAAATCG ACAACATGTG GCGTCAATTG 
AAGTCTACCT TTAACGAGAC CCTCCGTTCC AGTCCCGCCT TGAATTGGAT TGAGCGACCC 
ACCCGGAATC TTGCTATGGC GAAGCTACAG GCAATGACGC TGGAGGTCAA CAACTACGCA 
GACGACAACT TCACCGAGGA GTTTGCCGAA CTCAATCTGC AAAGTGACGA CTACGTGGAG 
AATGTGCGAT ATACAAGCCT ACTTGGGGCC AAACAGATGC GGGAGATGCT TCACAAGCCG 
GCCAAACCCT TTGAGGCGGG CTCCCAACTC AGTTACACCC CGGCCAACAT CCTGATAGAG 
AACACCATCA AGGTTCCGGT GGCCCTGCTA CAACCCTTCT ACATTTGGTC CGATGTCTAC 
CCCAATGCGA TTATGTTCGG AACCCTGGCC TCCCTAATTG GACACGAGTT AATCCACGGA 
TTCGACGACA GCGGGCGGAA GTTCGATGAG AAGGGAAACT CGAAGGATTG GTGGGATGAG 
AAGTCCAGCA GCAACTTCCT GAAACGAAGG GACTGCTTCA CCAAGCAGTA TGGCAGGTAT 
GTCTACGATG GCATTCAGTT AAAGGAGTCA ACCGCGCAGT CGGAGAACAT TGCGGACAAC 
GGCGGAATGC GACTGGCCTA CACCGCCTAC CGGAAATGGT ACGAAAACCA GTTGACACTT 
CCCAATGGGG CTCAGGATAT GACCAAGGAG ACCCTGCCCA ACCTGCGATA CACCGCCAAA 
CAACTATTCT TCATTAGCTT TGCTCAGAGC TGGTGCAATG ATGCACATCC CAGTGTGAAG 
GCACTTCAGG TGTCAACCGA CCAGCACATG CCCGGAAGAT TCCGCGTTAT TGGATCTTTA 
TCTAACTTCG AGGAGTTCTC GAAGGAGTTC AACTGCCCAG CCGGATCCGC CATGAATCCT 
AGTGAGAAAT GCATACTTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nepl21 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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