Nepl17 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nepl17
Entrez Gene ID	43408
Full Name	CG14529 gene product from transcript CG14529-RA
Synonyms	Dmel\CG14529
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98F1-98F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_143384.2	NP_651641.1	Neprilysin-like 17

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG14527	NP_651645.1
Drosophila melanogaster (fruit fly)	CG14526	NP_651646.2
Drosophila melanogaster (fruit fly)	CG4721	NP_651098.2
Drosophila melanogaster (fruit fly)	CG14523	NP_651644.1
Drosophila melanogaster (fruit fly)	CG14529	NP_651641.1
Drosophila melanogaster (fruit fly)	CG14528	NP_651643.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nepl17 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nepl17 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06423	NM_143384.2 Latest version!	Drosophila melanogaster Neprilysin-like 17 (Nepl17), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nepl17 ( NM_143384.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651641.1 Drosophila melanogaster Neprilysin-like 17 (Nepl17), mRNA.

Price & Availability↑

CloneID	OFa06423
Clone ID Related Accession (Same CDS sequence)	NM_143384.2
Accession Version	NM_143384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2046bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Neprilysin-like 17
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_143384.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACGCC TCCCAGCTTC CGCCTGCCTA ATCTTTGTCA CCTTGGCCGT CGGTTCATTG 
GCCATCCAGG GTGGAGTCTT CAAGGAGCTA ACCACGCCTC TGGGTCAGCA GATCATGCGT 
ATAGCCAAGT CGGCGGAGAT GTGCTCCTTT ATGGACACCT CTGTGAGTCC CTGCGACGAT 
TTCTATGGCT ATGCCTGCGG GAATTATGCG ACAATAAATG CGGCAACCTC GCAGAAAGAC 
ACCAATATCC GGCAGCTTAT GTTTGCCAAC TATCTGCGTC GGGTGAGGCA GGTGCTTAAC 
GAGCCCCGGA TAAGTACAGA TCGGCCGATG GAGGTGCGCG TGAAGTATTT CTACGAATCC 
TGCCTGGACA CAGCGGCTTT AAGGACTAAA CAGCGATCCC ATCTGCTCAG TGTTCTAAGG 
GAGTTTGGTG GAATGCCGGC GGTGGAGGGC AAGTCCTGGG ACTACGTCGG TTTCGACGCC 
ATTGAAATGA TGGCTCAGTT GCTCCGCCGC TACGGAAAGT TGACTCTACT GGGTGTCTAT 
GTGGTTCCGG ATCACTTCAA CTCCCAGATT AAACGCCTAC AGTTGGGCCA AAGGCTCGAT 
CTCGCTGAGA GCGATGAGTT TGCCAGTCTG TGGAAGAAGT ATGCGCTGAA GATGCGTCTG 
CGGAACCTCC TGGGTCTGTC GGAGGAGCTA GCTAGAAAGA CTGCTGAGGA GATAATAGAG 
CTGGAGTTGG AACTATCGCG CAGTGCTTTT GACCATAGAA TGAACCGTGA TCCGCGACTG 
ATGCGCCATC TGACCATGCT GTCCAACATG AGCGATGCTT ACGGACCGAT TCTTAACGTG 
ACTCGCTTTG TGACCTCCTG GTTGGGTTCC GAATACAATC TTCCGGTTTT TGAATCAGTT 
CCTAGCTACC TTTTCCAGGT AAAGAAGATC CTGCTGAGCA CTCCCAACCA TGTGGTGGCC 
AACTATATGC TGTCCAGTCT GTTGACCGAC TTTGAGATCA CGGCGAATGA AGAGCAACAG 
AAAGTGATCT GTCACGAGCG GATCACCGAG CTTTTTCCCG ATGTGGTGGA CCATATGGTG 
TACCACTCAT TAGAGCAGCA AAATCCCTAC ATAGCCAACG AGATGAGGAG TCTGTGGGTG 
GAGCTGAAGT CCTCCTTCCG CGAAATGCTC AGTTCCCCGG ACATCGAATG GCTGGAGAAG 
CAGACACGTG AGGAGTTACT GGAAAAACTC AATGGTATGT CCTTCGAGAT AGCCGGCGGT 
CAGGCGGTCA ACTTCGAGGA GCAGTTCGGC GATCTGGTGG TCAGCTCCGC CGATTATTAT 
GGCAATATCC AACGCCTGCT GGAGGTAAAG GCCAGAATTC TCCGCACGGA TCTTTTGCGG 
GAGTCCAGCA GCGTATACTA CTACCACGGT GTAACCGCGA CTCCTATCTA TGAGACGGAA 
ACAAATCTGG TGGTGCTACC GGTCAGCTAT ATGCAGCATC GCTATCTTTG GGACGATATT 
TATCCGGCAG CTCTTAAGTA CGGCACTCTG GGTTTCACCA TGGCTCACGA GATGGCCCAC 
GGATTCGATG ATCTGAACCG GCTCTACGAC AGCAGGGGAA ACCTCAACGA CAACTGGAGC 
ACCCAGACCA AGGTGGGTTT TGAGCTGGTC AAAAATTGCC TGGCGGACCA GTTCAGTGGG 
ATGCTGTACG GCAACTTGCG ATTGCGAAGA TTGAAGTCGC AAGCTGAGAG TATAGCGGAC 
AATGTGGGTA TTCGCATTGC GCAGTCGGCG TACTGGAAAT GGTTGGATCA AGTAGAGATG 
GAGGAAACGG AAAGCCTGCC GCACATGACG CAGTCGCCGG AACAGCTTTT CTTCCTCAGC 
GCGGCTCAGT TCATGTGCTC GGATATCTAT CCAGAAAGAC GAGCGAACTT CGTCCGAAAC 
AATTTCCATC CGCCATATGA GGCCAGGGTA TTCGCCATGA TGATCAACAG CCCAGCCTTC 
GCCGAGGCAT TCCAGTGCAG CAACTCGTCC AAGATGAATC CCCCAAACAA GTGTCTGATG 
TATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651641.1
CDS	11..2056
Translation	MERLPASACLIFVTLAVGSLAIQGGVFKELTTPLGQQIMRIAKSAEMCSFMDTSVSPCDDFYGYACGNYATINAATSQKDTNIRQLMFANYLRRVRQVLNEPRISTDRPMEVRVKYFYESCLDTAALRTKQRSHLLSVLREFGGMPAVEGKSWDYVGFDAIEMMAQLLRRYGKLTLLGVYVVPDHFNSQIKRLQLGQRLDLAESDEFASLWKKYALKMRLRNLLGLSEELARKTAEEIIELELELSRSAFDHRMNRDPRLMRHLTMLSNMSDAYGPILNVTRFVTSWLGSEYNLPVFESVPSYLFQVKKILLSTPNHVVANYMLSSLLTDFEITANEEQQKVICHERITELFPDVVDHMVYHSLEQQNPYIANEMRSLWVELKSSFREMLSSPDIEWLEKQTREELLEKLNGMSFEIAGGQAVNFEEQFGDLVVSSADYYGNIQRLLEVKARILRTDLLRESSSVYYYHGVTATPIYETETNLVVLPVSYMQHRYLWDDIYPAALKYGTLGFTMAHEMAHGFDDLNRLYDSRGNLNDNWSTQTKVGFELVKNCLADQFSGMLYGNLRLRRLKSQAESIADNVGIRIAQSAYWKWLDQVEMEETESLPHMTQSPEQLFFLSAAQFMCSDIYPERRANFVRNNFHPPYEARVFAMMINSPAFAEAFQCSNSSKMNPPNKCLMY

Target ORF information:

RefSeq Version	NM_143384.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Neprilysin-like 17 (Nepl17), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143384.2

ORF Insert Sequence:

ATGGAACGCC TCCCAGCTTC CGCCTGCCTA ATCTTTGTCA CCTTGGCCGT CGGTTCATTG 
GCCATCCAGG GTGGAGTCTT CAAGGAGCTA ACCACGCCTC TGGGTCAGCA GATCATGCGT 
ATAGCCAAGT CGGCGGAGAT GTGCTCCTTT ATGGACACCT CTGTGAGTCC CTGCGACGAT 
TTCTATGGCT ATGCCTGCGG GAATTATGCG ACAATAAATG CGGCAACCTC GCAGAAAGAC 
ACCAATATCC GGCAGCTTAT GTTTGCCAAC TATCTGCGTC GGGTGAGGCA GGTGCTTAAC 
GAGCCCCGGA TAAGTACAGA TCGGCCGATG GAGGTGCGCG TGAAGTATTT CTACGAATCC 
TGCCTGGACA CAGCGGCTTT AAGGACTAAA CAGCGATCCC ATCTGCTCAG TGTTCTAAGG 
GAGTTTGGTG GAATGCCGGC GGTGGAGGGC AAGTCCTGGG ACTACGTCGG TTTCGACGCC 
ATTGAAATGA TGGCTCAGTT GCTCCGCCGC TACGGAAAGT TGACTCTACT GGGTGTCTAT 
GTGGTTCCGG ATCACTTCAA CTCCCAGATT AAACGCCTAC AGTTGGGCCA AAGGCTCGAT 
CTCGCTGAGA GCGATGAGTT TGCCAGTCTG TGGAAGAAGT ATGCGCTGAA GATGCGTCTG 
CGGAACCTCC TGGGTCTGTC GGAGGAGCTA GCTAGAAAGA CTGCTGAGGA GATAATAGAG 
CTGGAGTTGG AACTATCGCG CAGTGCTTTT GACCATAGAA TGAACCGTGA TCCGCGACTG 
ATGCGCCATC TGACCATGCT GTCCAACATG AGCGATGCTT ACGGACCGAT TCTTAACGTG 
ACTCGCTTTG TGACCTCCTG GTTGGGTTCC GAATACAATC TTCCGGTTTT TGAATCAGTT 
CCTAGCTACC TTTTCCAGGT AAAGAAGATC CTGCTGAGCA CTCCCAACCA TGTGGTGGCC 
AACTATATGC TGTCCAGTCT GTTGACCGAC TTTGAGATCA CGGCGAATGA AGAGCAACAG 
AAAGTGATCT GTCACGAGCG GATCACCGAG CTTTTTCCCG ATGTGGTGGA CCATATGGTG 
TACCACTCAT TAGAGCAGCA AAATCCCTAC ATAGCCAACG AGATGAGGAG TCTGTGGGTG 
GAGCTGAAGT CCTCCTTCCG CGAAATGCTC AGTTCCCCGG ACATCGAATG GCTGGAGAAG 
CAGACACGTG AGGAGTTACT GGAAAAACTC AATGGTATGT CCTTCGAGAT AGCCGGCGGT 
CAGGCGGTCA ACTTCGAGGA GCAGTTCGGC GATCTGGTGG TCAGCTCCGC CGATTATTAT 
GGCAATATCC AACGCCTGCT GGAGGTAAAG GCCAGAATTC TCCGCACGGA TCTTTTGCGG 
GAGTCCAGCA GCGTATACTA CTACCACGGT GTAACCGCGA CTCCTATCTA TGAGACGGAA 
ACAAATCTGG TGGTGCTACC GGTCAGCTAT ATGCAGCATC GCTATCTTTG GGACGATATT 
TATCCGGCAG CTCTTAAGTA CGGCACTCTG GGTTTCACCA TGGCTCACGA GATGGCCCAC 
GGATTCGATG ATCTGAACCG GCTCTACGAC AGCAGGGGAA ACCTCAACGA CAACTGGAGC 
ACCCAGACCA AGGTGGGTTT TGAGCTGGTC AAAAATTGCC TGGCGGACCA GTTCAGTGGG 
ATGCTGTACG GCAACTTGCG ATTGCGAAGA TTGAAGTCGC AAGCTGAGAG TATAGCGGAC 
AATGTGGGTA TTCGCATTGC GCAGTCGGCG TACTGGAAAT GGTTGGATCA AGTAGAGATG 
GAGGAAACGG AAAGCCTGCC GCACATGACG CAGTCGCCGG AACAGCTTTT CTTCCTCAGC 
GCGGCTCAGT TCATGTGCTC GGATATCTAT CCAGAAAGAC GAGCGAACTT CGTCCGAAAC 
AATTTCCATC CGCCATATGA GGCCAGGGTA TTCGCCATGA TGATCAACAG CCCAGCCTTC 
GCCGAGGCAT TCCAGTGCAG CAACTCGTCC AAGATGAATC CCCCAAACAA GTGTCTGATG 
TATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nepl17 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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