Nepl19 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nepl19
Entrez Gene ID	43411
Full Name	CG14523 gene product from transcript CG14523-RA
Synonyms	Dmel\CG14523
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98F1-98F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_143387.2	NP_651644.1	Neprilysin-like 19

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG14527	NP_651645.1
Drosophila melanogaster (fruit fly)	CG14526	NP_651646.2
Drosophila melanogaster (fruit fly)	CG4721	NP_651098.2
Drosophila melanogaster (fruit fly)	CG14523	NP_651644.1
Drosophila melanogaster (fruit fly)	CG14529	NP_651641.1
Drosophila melanogaster (fruit fly)	CG14528	NP_651643.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nepl19 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nepl19 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06425	NM_143387.2 Latest version!	Drosophila melanogaster Neprilysin-like 19 (Nepl19), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nepl19 ( NM_143387.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651644.1 Drosophila melanogaster Neprilysin-like 19 (Nepl19), mRNA.

Price & Availability↑

CloneID	OFa06425
Clone ID Related Accession (Same CDS sequence)	NM_143387.2
Accession Version	NM_143387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Neprilysin-like 19
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143387.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACT TTGGTATCGC ACATGCCCTG CTCCAGCTAC TGCTGCTCCA TCTGGTGGGA 
AGTGGTCGAG GTCACCTGCT AACTGGTCAA CCGGCCATTG ACCTGCGCAT CCAGGCACGG 
GAGCAGCTGA TCTCGCAGTC CAATGACAGC GCCTATGTCC AGCGACTGAT GCGTCTGTCC 
AAGTCCGCCG AAATGCGCAG CTATATGCAG CAGGACGCGG ATGCCTGCGA AAACTTCTAC 
GACTACAGCT GCGGAAATTG GCCCCAGATC AATCCGGCCA ACGATGCCTA TCCCCGGGAG 
ACGAACTTCG TGCAGCTGCT GCTCAAGGCC TATAGCCACA AGCAGCAGCG TCTAATGGAG 
CAGCCGGCGA ACGTGGAAAC GGATGAGGTG GCCGTGCTCC GACTGAAGGA GTTCTATGCC 
AGTTGCCTGC TCTTTCGACA GGTACCGGAG GATCTCTACA GACGTCAGTT GCAGGAAATT 
GTGGCTGATT TCGGACGCAT GCCCGTCCTA AGTCTCCCGG GTCAGGAGTG GCCAGCCGAG 
GAGTTCGATT GGCTGGACAC AGTGGCGCGA ATAAAGCGGC AGTACGGCTT TGACATCCTT 
TTGCTCTTTC AGCAGTCGGC GGATCGCATA TACGTGGGAC AGCCGAAGCA GATTCTTCCG 
GAGGCCAACA GAAAGGCCGT GGCAGAAGAT ATAGCCAATC ATTTGGAACG TCACTTGGGT 
GTGGATCCCA AAGTGGCCGT AACAACGGCA AGGGAAATTA CAGAGTTTGA GCAAAAGATT 
GCCGCCATTA TGGTGGATCG TCGAGTTGGA GTGATGTCCA AATTGTACAC TCCAAGTGAC 
GCGGACTACG TTGATGTCTC AAAACTCACC CAGTTCGTGG AAACTGTCCT TGACCGGGAG 
TTGTTGGCCA ACGAGTCCCT ATATCAGCAT GTTCCTGATT ATCTTATGAA AATAGATGAG 
CTGGTGACGG AGACACCACC ACACGTACTG GCTAACTATG TCTTCAATGA GCTTTTAAAG 
CACTTCTACT ACGAGCGAGC GGAATCCGTT GTGGAGCAGT GCGTCAGTCG GGTCAGGGAT 
CTCTTTGGCG AGCTCCTTGA CCACATGTTG TTCGAGCAGT ATGAAGATGA AGGTACCCTG 
AAGGATATAT ATGTAGTCTG GCAGCAGTTA CAGCGCAGCT TTAGAGGTGT GTTACAAAAC 
TCTTCAGCAA ATTGGTTGGT TCCGGAGACG AGGGAACAAC TACTAGAGCA GTTGAATACA 
ACCAGTCTGG TGATCAATGG ATATGCCGAA GTCAATTTCA CGCAGCGATA TGAGGAGCTG 
CACCTGAAAC CTAGAGATTA TCTTCACAAT CTGAGGACCA TACTTAACCA TCAGAGTTTG 
AGGGTCAAGG TGAAGACTCA GACATCGGTC ACCTATGATC CTGTGGGAAA GAGAGTCCTG 
CTACCAGTTG CTCTGCTCCA ACCGAACTTC CTGTGGTCCC GCTACTATCC AAAAGCAGTG 
CGGTATGGCA GTCTGGGCAC CTTGCTGGCC CAGCAGTTGG CACACAGCTT GGAAGATGCA 
TCCAAGTGGG ATGCCAAGTC CTTTGCTGAG TACTCCAAAA GGAAGTCCTG CTTCAAGGAG 
CAGTACGGTA GACTGCGGCT CAATGGACAT TACTTGCCGG AGAGCGATCT GCAGGCGGAG 
AACATAGCCG ACAATCTGGC CATTCAGGTG GCCTACCATG CCTACCGAAG GCTTCTGGCG 
GAACTGGACC CATCCTTCTT GGGCTCCGAG TCCTTGCCAC AACTCAACCT TGGCTCCCGA 
AGACTGTTCT TCCTCAGCTT TGCCCAGTTA TGGTGCAATG ATGCCAATGA ACAGTTCCGG 
GACAAGCAAT CCCTGTTTTT GGGCACTCCC AATGCCCTCC GCGTGCTGGG AGCACTATCG 
AACTTTAGAG CCTTTGGACG GGACTTTAAC TGCGGGACCG CTAGCAGGAT GACCTCTCCC 
CAGAAGTGTC AGCTCTTCGC CGTTAACCTG GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651644.1
CDS	27..2042
Translation	MENFGIAHALLQLLLLHLVGSGRGHLLTGQPAIDLRIQAREQLISQSNDSAYVQRLMRLSKSAEMRSYMQQDADACENFYDYSCGNWPQINPANDAYPRETNFVQLLLKAYSHKQQRLMEQPANVETDEVAVLRLKEFYASCLLFRQVPEDLYRRQLQEIVADFGRMPVLSLPGQEWPAEEFDWLDTVARIKRQYGFDILLLFQQSADRIYVGQPKQILPEANRKAVAEDIANHLERHLGVDPKVAVTTAREITEFEQKIAAIMVDRRVGVMSKLYTPSDADYVDVSKLTQFVETVLDRELLANESLYQHVPDYLMKIDELVTETPPHVLANYVFNELLKHFYYERAESVVEQCVSRVRDLFGELLDHMLFEQYEDEGTLKDIYVVWQQLQRSFRGVLQNSSANWLVPETREQLLEQLNTTSLVINGYAEVNFTQRYEELHLKPRDYLHNLRTILNHQSLRVKVKTQTSVTYDPVGKRVLLPVALLQPNFLWSRYYPKAVRYGSLGTLLAQQLAHSLEDASKWDAKSFAEYSKRKSCFKEQYGRLRLNGHYLPESDLQAENIADNLAIQVAYHAYRRLLAELDPSFLGSESLPQLNLGSRRLFFLSFAQLWCNDANEQFRDKQSLFLGTPNALRVLGALSNFRAFGRDFNCGTASRMTSPQKCQLFAVNLD

Target ORF information:

RefSeq Version	NM_143387.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Neprilysin-like 19 (Nepl19), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143387.2

ORF Insert Sequence:

ATGGAGAACT TTGGTATCGC ACATGCCCTG CTCCAGCTAC TGCTGCTCCA TCTGGTGGGA 
AGTGGTCGAG GTCACCTGCT AACTGGTCAA CCGGCCATTG ACCTGCGCAT CCAGGCACGG 
GAGCAGCTGA TCTCGCAGTC CAATGACAGC GCCTATGTCC AGCGACTGAT GCGTCTGTCC 
AAGTCCGCCG AAATGCGCAG CTATATGCAG CAGGACGCGG ATGCCTGCGA AAACTTCTAC 
GACTACAGCT GCGGAAATTG GCCCCAGATC AATCCGGCCA ACGATGCCTA TCCCCGGGAG 
ACGAACTTCG TGCAGCTGCT GCTCAAGGCC TATAGCCACA AGCAGCAGCG TCTAATGGAG 
CAGCCGGCGA ACGTGGAAAC GGATGAGGTG GCCGTGCTCC GACTGAAGGA GTTCTATGCC 
AGTTGCCTGC TCTTTCGACA GGTACCGGAG GATCTCTACA GACGTCAGTT GCAGGAAATT 
GTGGCTGATT TCGGACGCAT GCCCGTCCTA AGTCTCCCGG GTCAGGAGTG GCCAGCCGAG 
GAGTTCGATT GGCTGGACAC AGTGGCGCGA ATAAAGCGGC AGTACGGCTT TGACATCCTT 
TTGCTCTTTC AGCAGTCGGC GGATCGCATA TACGTGGGAC AGCCGAAGCA GATTCTTCCG 
GAGGCCAACA GAAAGGCCGT GGCAGAAGAT ATAGCCAATC ATTTGGAACG TCACTTGGGT 
GTGGATCCCA AAGTGGCCGT AACAACGGCA AGGGAAATTA CAGAGTTTGA GCAAAAGATT 
GCCGCCATTA TGGTGGATCG TCGAGTTGGA GTGATGTCCA AATTGTACAC TCCAAGTGAC 
GCGGACTACG TTGATGTCTC AAAACTCACC CAGTTCGTGG AAACTGTCCT TGACCGGGAG 
TTGTTGGCCA ACGAGTCCCT ATATCAGCAT GTTCCTGATT ATCTTATGAA AATAGATGAG 
CTGGTGACGG AGACACCACC ACACGTACTG GCTAACTATG TCTTCAATGA GCTTTTAAAG 
CACTTCTACT ACGAGCGAGC GGAATCCGTT GTGGAGCAGT GCGTCAGTCG GGTCAGGGAT 
CTCTTTGGCG AGCTCCTTGA CCACATGTTG TTCGAGCAGT ATGAAGATGA AGGTACCCTG 
AAGGATATAT ATGTAGTCTG GCAGCAGTTA CAGCGCAGCT TTAGAGGTGT GTTACAAAAC 
TCTTCAGCAA ATTGGTTGGT TCCGGAGACG AGGGAACAAC TACTAGAGCA GTTGAATACA 
ACCAGTCTGG TGATCAATGG ATATGCCGAA GTCAATTTCA CGCAGCGATA TGAGGAGCTG 
CACCTGAAAC CTAGAGATTA TCTTCACAAT CTGAGGACCA TACTTAACCA TCAGAGTTTG 
AGGGTCAAGG TGAAGACTCA GACATCGGTC ACCTATGATC CTGTGGGAAA GAGAGTCCTG 
CTACCAGTTG CTCTGCTCCA ACCGAACTTC CTGTGGTCCC GCTACTATCC AAAAGCAGTG 
CGGTATGGCA GTCTGGGCAC CTTGCTGGCC CAGCAGTTGG CACACAGCTT GGAAGATGCA 
TCCAAGTGGG ATGCCAAGTC CTTTGCTGAG TACTCCAAAA GGAAGTCCTG CTTCAAGGAG 
CAGTACGGTA GACTGCGGCT CAATGGACAT TACTTGCCGG AGAGCGATCT GCAGGCGGAG 
AACATAGCCG ACAATCTGGC CATTCAGGTG GCCTACCATG CCTACCGAAG GCTTCTGGCG 
GAACTGGACC CATCCTTCTT GGGCTCCGAG TCCTTGCCAC AACTCAACCT TGGCTCCCGA 
AGACTGTTCT TCCTCAGCTT TGCCCAGTTA TGGTGCAATG ATGCCAATGA ACAGTTCCGG 
GACAAGCAAT CCCTGTTTTT GGGCACTCCC AATGCCCTCC GCGTGCTGGG AGCACTATCG 
AACTTTAGAG CCTTTGGACG GGACTTTAAC TGCGGGACCG CTAGCAGGAT GACCTCTCCC 
CAGAAGTGTC AGCTCTTCGC CGTTAACCTG GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nepl19 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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