Nepl20 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nepl20
Entrez Gene ID	43412
Full Name	CG14527 gene product from transcript CG14527-RA
Synonyms	Dmel\CG14527
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98F1-98F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_143388.2	NP_651645.1	Neprilysin-like 20

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG14527	NP_651645.1
Drosophila melanogaster (fruit fly)	CG14526	NP_651646.2
Drosophila melanogaster (fruit fly)	CG4721	NP_651098.2
Drosophila melanogaster (fruit fly)	CG14523	NP_651644.1
Drosophila melanogaster (fruit fly)	CG14529	NP_651641.1
Drosophila melanogaster (fruit fly)	CG14528	NP_651643.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nepl20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nepl20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06426	NM_143388.2 Latest version!	Drosophila melanogaster Neprilysin-like 20 (Nepl20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nepl20 ( NM_143388.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651645.1 Drosophila melanogaster Neprilysin-like 20 (Nepl20), mRNA.

Price & Availability↑

CloneID	OFa06426
Clone ID Related Accession (Same CDS sequence)	NM_143388.2
Accession Version	NM_143388.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Neprilysin-like 20
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143388.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGATGA AATTGTTCTG CATTTGGATT GCTAGTCACG TTCTCAGTGT TCTGGTTTTA 
GCGGCCCCCA CAAGCGAGGA TGTACTCAAC CAGGACACGC CTTACATGAA GGAACTAATG 
CGTCAAGCCA AAGCTGCTGA GATCGAGAGC TTTATGAACC AGAAGGTGGA TCCGTGCAAG 
GATTTCTACG CATTCTCCTG TGGCAACTAT AAACGCATCA ACTCGGCTTT GAACATGCGG 
GTATTGACTA CTGGAGTGTT CGAGACTTTG ACAAAAGGGC TCAACCGAAA GATCCTGAAG 
ATGCTCAACA CACCCCATGA TAGCCACGAC ACTCCGGAGG ACATTCAGGT GAAGCACTTC 
TACGAGTCCT GTCTCCAGAT CAAGGAGCTC AACTCGACGT ACAGCGAAAA GCTGAAGCGG 
CTCATTGCAG AATTTGGAAC GATGCCGCTG CTGGAAGGAT CCTCCTGGCA GGAGGACGAC 
TTTGACTGGC TGAATACTAC TGCTCGCATG GCCCACCGAT ACGGAATAAT GTCCATTATA 
GGCGTTGGAG TCGCCACGGA CTTCGCCAAT AACCAAAAAA ACATTATTTA CATAAACCAG 
CAGGAGTTCC CATTAAAGAC ACGTTCCATG TATATGGATA ACGAAACGGC CATCTACCGC 
CAAAATTATC AGAATAATAT CCAGCTCATT TTGGAGAAAT ACTTAGGTGT TAAGGACGAA 
TTGGCAAGAA AAGCGGCCAA GGAAATGTTT GAATTCGAGG TGGACTTGGC CCATGGACTG 
GTGGACAACA ACAAACACCT GAACCTAAGG GACCTGATCG AACTTCTGAC TGTCGCTGAA 
TTGCGCGAGA GATATGCCCC CACCTTGGAC ACCGACCAAT TGATATTCGT GAGCATGGGA 
GAGAAGATTT CCGACAAGGT CTACGAATAC AACAGACGTT ATCAGCAAAA TTTGGTCGAG 
GTGATCGAGC GTACGCCCAA GAGCACTGTG GCCAACTATT TATTCCTTCG CCTGATCTGG 
GAGTTTATAC AGAAACCAAG TGGAGTGGCG GAAAAGCCGA CAGAGGCCTG TGTGGACTTC 
ACCAAGACGT ACTTCGCGAA GAATCTGGAC AACATGATCT ACCGCCGCTA CAAAAACGAG 
AAATCCTCCA GAGAAATCGA CAGCATGTGG CATCAATTGA AGTCCACCTT TAAGGAGACC 
TTGCTATCCA GTCCGGCTCT AGATTGGATC GAGCCATCCA CTCGAAGTCT TGCCATCGCT 
AAGCTGGAGG CTATGACCCT GGAGGTCAAC AACTACGTTA AGGAGAACTT TACCGAGGAA 
TTTGCGGACC TCAATTTGCA AAACACGGAC TGCGTAGAGA ATTTGCGACA GGTTCAGATG 
CTGCAAGCAA AGCAAATGCG GCAATTGTAT CACCAACCGG CTAAGCCACT GGAAGCGGGC 
GAACTTTTGA GCTATACCCC AAGCAACATC CTGCTGGAGA ACAGCATTAA GGTTCCGGTG 
GCCCTGCTAC AACCCTTCTA CATTTGGTCC GATGTCTACC CCAATGCGGT TATGTTCGGA 
ACCCTGGCCT ACCTAATTGG ACACGAGTTA ATCCACGGAT TCGACGACAG CGGACGGAAG 
TTTGACGAGA AGGGGAACTC AAAGGATTGG TGGGATGAGA AGTCCAGCAG CAACTTCCTA 
AAACGACGGG ATTGCTTCAC CAAGCAGTAT GGCAGGTATG TCTACGATGG CATTCAGTTG 
AAGGAGTCAA CCGCGCAGTC GGAGAACATT GCGGACAACG GCGGAATGCG ACTGGCCTAC 
ACCGCCTACC GGAAATGGTA CGAAAACCAG TTGACACTTC CTAATGGGGC TCAGGATATG 
ACCAAGGAGA CCCTGCCCAA CCTGCGATAC ACCGCCAAAC AACTATTCTT CATTAGCTTT 
GCTCAGAGCT GGTGCAACGA TGTACATCCC AAGGTGAAGG CCCTTCAGGT GTCAACCGAC 
GACCACATGC CAGGAAAGTT CCGCGTTATT GGAACTTTAT CTAACTTTGA GGAGTTTTCA 
AAGGAGTTCA ACTGTCACGC CGGATCCGCC ATGAATCCCA TCGAAAAATG CATACTTTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651645.1
CDS	12..2114
Translation	MTMKLFCIWIASHVLSVLVLAAPTSEDVLNQDTPYMKELMRQAKAAEIESFMNQKVDPCKDFYAFSCGNYKRINSALNMRVLTTGVFETLTKGLNRKILKMLNTPHDSHDTPEDIQVKHFYESCLQIKELNSTYSEKLKRLIAEFGTMPLLEGSSWQEDDFDWLNTTARMAHRYGIMSIIGVGVATDFANNQKNIIYINQQEFPLKTRSMYMDNETAIYRQNYQNNIQLILEKYLGVKDELARKAAKEMFEFEVDLAHGLVDNNKHLNLRDLIELLTVAELRERYAPTLDTDQLIFVSMGEKISDKVYEYNRRYQQNLVEVIERTPKSTVANYLFLRLIWEFIQKPSGVAEKPTEACVDFTKTYFAKNLDNMIYRRYKNEKSSREIDSMWHQLKSTFKETLLSSPALDWIEPSTRSLAIAKLEAMTLEVNNYVKENFTEEFADLNLQNTDCVENLRQVQMLQAKQMRQLYHQPAKPLEAGELLSYTPSNILLENSIKVPVALLQPFYIWSDVYPNAVMFGTLAYLIGHELIHGFDDSGRKFDEKGNSKDWWDEKSSSNFLKRRDCFTKQYGRYVYDGIQLKESTAQSENIADNGGMRLAYTAYRKWYENQLTLPNGAQDMTKETLPNLRYTAKQLFFISFAQSWCNDVHPKVKALQVSTDDHMPGKFRVIGTLSNFEEFSKEFNCHAGSAMNPIEKCILY

Target ORF information:

RefSeq Version	NM_143388.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Neprilysin-like 20 (Nepl20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143388.2

ORF Insert Sequence:

ATGACGATGA AATTGTTCTG CATTTGGATT GCTAGTCACG TTCTCAGTGT TCTGGTTTTA 
GCGGCCCCCA CAAGCGAGGA TGTACTCAAC CAGGACACGC CTTACATGAA GGAACTAATG 
CGTCAAGCCA AAGCTGCTGA GATCGAGAGC TTTATGAACC AGAAGGTGGA TCCGTGCAAG 
GATTTCTACG CATTCTCCTG TGGCAACTAT AAACGCATCA ACTCGGCTTT GAACATGCGG 
GTATTGACTA CTGGAGTGTT CGAGACTTTG ACAAAAGGGC TCAACCGAAA GATCCTGAAG 
ATGCTCAACA CACCCCATGA TAGCCACGAC ACTCCGGAGG ACATTCAGGT GAAGCACTTC 
TACGAGTCCT GTCTCCAGAT CAAGGAGCTC AACTCGACGT ACAGCGAAAA GCTGAAGCGG 
CTCATTGCAG AATTTGGAAC GATGCCGCTG CTGGAAGGAT CCTCCTGGCA GGAGGACGAC 
TTTGACTGGC TGAATACTAC TGCTCGCATG GCCCACCGAT ACGGAATAAT GTCCATTATA 
GGCGTTGGAG TCGCCACGGA CTTCGCCAAT AACCAAAAAA ACATTATTTA CATAAACCAG 
CAGGAGTTCC CATTAAAGAC ACGTTCCATG TATATGGATA ACGAAACGGC CATCTACCGC 
CAAAATTATC AGAATAATAT CCAGCTCATT TTGGAGAAAT ACTTAGGTGT TAAGGACGAA 
TTGGCAAGAA AAGCGGCCAA GGAAATGTTT GAATTCGAGG TGGACTTGGC CCATGGACTG 
GTGGACAACA ACAAACACCT GAACCTAAGG GACCTGATCG AACTTCTGAC TGTCGCTGAA 
TTGCGCGAGA GATATGCCCC CACCTTGGAC ACCGACCAAT TGATATTCGT GAGCATGGGA 
GAGAAGATTT CCGACAAGGT CTACGAATAC AACAGACGTT ATCAGCAAAA TTTGGTCGAG 
GTGATCGAGC GTACGCCCAA GAGCACTGTG GCCAACTATT TATTCCTTCG CCTGATCTGG 
GAGTTTATAC AGAAACCAAG TGGAGTGGCG GAAAAGCCGA CAGAGGCCTG TGTGGACTTC 
ACCAAGACGT ACTTCGCGAA GAATCTGGAC AACATGATCT ACCGCCGCTA CAAAAACGAG 
AAATCCTCCA GAGAAATCGA CAGCATGTGG CATCAATTGA AGTCCACCTT TAAGGAGACC 
TTGCTATCCA GTCCGGCTCT AGATTGGATC GAGCCATCCA CTCGAAGTCT TGCCATCGCT 
AAGCTGGAGG CTATGACCCT GGAGGTCAAC AACTACGTTA AGGAGAACTT TACCGAGGAA 
TTTGCGGACC TCAATTTGCA AAACACGGAC TGCGTAGAGA ATTTGCGACA GGTTCAGATG 
CTGCAAGCAA AGCAAATGCG GCAATTGTAT CACCAACCGG CTAAGCCACT GGAAGCGGGC 
GAACTTTTGA GCTATACCCC AAGCAACATC CTGCTGGAGA ACAGCATTAA GGTTCCGGTG 
GCCCTGCTAC AACCCTTCTA CATTTGGTCC GATGTCTACC CCAATGCGGT TATGTTCGGA 
ACCCTGGCCT ACCTAATTGG ACACGAGTTA ATCCACGGAT TCGACGACAG CGGACGGAAG 
TTTGACGAGA AGGGGAACTC AAAGGATTGG TGGGATGAGA AGTCCAGCAG CAACTTCCTA 
AAACGACGGG ATTGCTTCAC CAAGCAGTAT GGCAGGTATG TCTACGATGG CATTCAGTTG 
AAGGAGTCAA CCGCGCAGTC GGAGAACATT GCGGACAACG GCGGAATGCG ACTGGCCTAC 
ACCGCCTACC GGAAATGGTA CGAAAACCAG TTGACACTTC CTAATGGGGC TCAGGATATG 
ACCAAGGAGA CCCTGCCCAA CCTGCGATAC ACCGCCAAAC AACTATTCTT CATTAGCTTT 
GCTCAGAGCT GGTGCAACGA TGTACATCCC AAGGTGAAGG CCCTTCAGGT GTCAACCGAC 
GACCACATGC CAGGAAAGTT CCGCGTTATT GGAACTTTAT CTAACTTTGA GGAGTTTTCA 
AAGGAGTTCA ACTGTCACGC CGGATCCGCC ATGAATCCCA TCGAAAAATG CATACTTTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nepl20 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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