Hsp60A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsp60A
Entrez Gene ID	32045
Full Name	Heat shock protein 60A
Synonyms	12,BP5,CG12101,Cpn60,Dm10A,DmHsp60,Dmel\CG12101,Dmhsp60,G62,HSP60,HSP60A,Hsp 60,Hsp60,Hsp64,IEF16,Mmp-P1,SSP 7506,cpn60,d-hsp60,hsp60,hsp60A,l(1)10Ac,l(1)BP5,l(1)G8,l(1)HM21,l(1)L12,l(1)dp025
General protein information	Preferred Names Heat shock protein 60A Names CG12101 gene product from transcript CG12101-RA 60-kDa heat shock protein, mitochondrial CG12101-PA CG12101-PB Hsp 60 Hsp60A-PA Hsp60A-PB heat shock protein 60 heat shock protein 60 kDa heat shock protein 60A lethal(1)10Ac mitochondrial matrix protein P1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 10A4-10A4\|1-33.51 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_078560.3	NP_511115.2	heat shock protein 60A, isoform A
NM_167266.1	NP_727489.1	heat shock protein 60A, isoform B

Pathways from BioSystems

Homologies

	HSPD1	XP_002799053.1
Danio rerio (zebrafish)	hspd1	NP_851847.1
Homo sapiens (human)	HSPD1	NP_955472.1
Gallus gallus (chicken)	HSPD1	NP_001012934.1
Saccharomyces cerevisiae (baker's yeast)	HSP60	NP_013360.1
Schizosaccharomyces pombe (fission yeast)	mcp60	NP_592894.1
Oryza sativa (rice)	Os10g0462900	NP_001064784.1
Pan troglodytes (chimpanzee)	HSPD1	XP_001169056.1
Mus musculus (house mouse)	Hspd1	NP_034607.3
Kluyveromyces lactis	KLLA0F09449g	XP_455510.1
Magnaporthe oryzae (rice blast fungus)	MGG_03165	XP_003716826.1
Arabidopsis thaliana (thale cress)	HSP60	NP_189041.1
Oryza sativa (rice)	Os03g0143400	NP_001048938.1
Canis lupus familiaris (dog)	HSPD1	XP_005640555.1
Bos taurus (cattle)	HSPD1	NP_001160082.1
Drosophila melanogaster (fruit fly)	Hsp60	NP_511115.2
Caenorhabditis elegans (roundworm)	hsp-60	NP_497429.1
Rattus norvegicus (Norway rat)	Hspd1	NP_071565.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004002	XP_318461.2
Eremothecium gossypii	AGOS_AFR155W	NP_985702.2
Neurospora crassa	NCU01589	XP_956500.2
Xenopus tropicalis (tropical clawed frog)	hspd1	NP_001096355.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We documented how floral traits under selection by multiple pollinators can result in either an intermediate "compromise" between selective pressures (sex organs) or apparent specialization (corolla tube length) to one pollinator
Title: Selection on Polemonium brandegeei (Polemoniaceae) flowers under hummingbird pollination: in opposition, parallel, or independent of selection by hawkmoths?

Expression of HSP60A is post-transcriptionally regulated in a highly dynamic pattern during embryogenesis, even under heat-shock conditions. In contrast, in very stressful situations, its expression is upregulated transcriptionally over the entire embryo.
Title: The expression of heat shock protein HSP60A reveals a dynamic mitochondrial pattern in Drosophila melanogaster embryos.

The following Hsp60A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsp60A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03472	NM_078560.3 Latest version!	Drosophila melanogaster heat shock protein 60A, transcript variant A (Hsp60A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa03472	NM_167266.1 Latest version!	Drosophila melanogaster heat shock protein 60A, transcript variant B (Hsp60A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsp60A ( NM_078560.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511115.2 Drosophila melanogaster heat shock protein 60A, transcript variant A (Hsp60A), mRNA.

Price & Availability↑

CloneID	OFa03472
Clone ID Related Accession (Same CDS sequence)	NM_078560.3 , NM_167266.1
Accession Version	NM_078560.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heat shock protein 60A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_078560.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCCGTT TGCCAGTTTC GCTTGCTCGC TCCTCCATTA GCCGCCAGTT GGCCATGCGC 
GGCTATGCCA AGGATGTGCG TTTCGGACCC GAGGTGCGCG CCATGATGCT CCAGGGCGTC 
GATGTGCTGG CCGATGCTGT GGCAGTGACC ATGGGCCCCA AGGGTCGCAA TGTGATCATT 
GAGCAGTCGT GGGGCTCGCC CAAAATCACC AAGGACGGCG TCACTGTGGC CAAGTCCATT 
GAGCTGAAGG ACAAGTTCCA GAACATCGGT GCCAAGCTGG TCCAGGATGT GGCCAACAAT 
ACCAACGAGG AGGCCGGTGA TGGCACCACC ACGGCCACCG TTCTGGCTCG CGCCATTGCC 
AAGGAGGGCT TCGAGAAGAT CTCCAAGGGC GCCAATCCCG TCGAGATTCG TCGCGGTGTC 
ATGCTGGCCG TTGAGACCGT CAAGGACAAC CTTAAGACCA TGTCGCGCCC CGTTAGCACG 
CCCGAGGAGA TCGCCCAGGT GGCCACCATC TCGGCCAACG GTGATCAGGC CATCGGCAAT 
CTCATCAGCG AGGCCATGAA GAAGGTGGGA CGCGATGGCG TTATCACCGT CAAGGATGGC 
AAGACCCTCA CCGATGAGTT GGAGGTTATC GAGGGCATGA AGTTCGATCG CGGCTACATC 
TCGCCGTACT TCATCAACTC CTCGAAGGGC GCCAAGGTGG AGTTCCAAGA TGCCCTCCTC 
CTGCTGTCCG AGAAGAAGAT CTCATCGGTG CAGAGCATCA TTCCCGCTCT GGAGCTGGCC 
AATGCGCAGC GCAAGCCGCT GGTCATCATT GCCGAGGATA TCGATGGTGA GGCCCTGAGC 
ACCCTGGTGG TGAACCGCCT CAAGATCGGC CTTCAGGTGG CTGCCGTCAA GGCACCCGGC 
TTCGGCGACA ACCGCAAGAG CACCCTCACC GATATGGCCA TCGCCTCCGG CGGCATTGTC 
TTCGGCGATG ATGCTGATCT CGTCAAGCTG GAGGACGTCA AGGTTAGCGA TTTGGGCCAG 
GTCGGCGAGG TGGTGATCAC CAAGGACGAC ACCCTGCTGC TGAAGGGCAA GGGCAAGAAG 
GACGATGTGC TCCGTCGCGC CAATCAGATC AAGGACCAGA TCGAGGACAC CACCTCCGAG 
TACGAGAAGG AGAAGCTGCA GGAGCGTCTG GCCCGCTTGG CCTCCGGCGT TGCCCTGCTG 
CGCGTTGGCG GCTCCAGCGA GGTGGAGGTC AACGAGAAGA AGGATCGCGT CCATGATGCC 
CTGAACGCCA CCCGTGCTGC CGTCGAAGAG GGAATCGTTC CCGGAGGCGG CACCGCTCTG 
CTGCGTTGCA TTGAGAAGCT CGAGGGTGTC GAGACCACCA ACGAGGATCA GAAACTCGGC 
GTGGAGATTG TGCGTCGTGC CCTGCGCATG CCATGCATGA CGATTGCCAA GAACGCCGGC 
GTTGATGGCG CCATGGTGGT GGCCAAGGTG GAGAATCAGG CCGGTGACTA CGGCTACGAT 
GCGCTGAAGG GCGAATACGG CAACCTGATC GAGAAGGGCA TCATCGATCC CACCAAGGTG 
GTGCGCACCG CCATCACAGA CGCCTCCGGT GTGGCCTCAC TGCTGACCAC CGCCGAGGCT 
GTCGTCACCG AGATCCCCAA GGAGGATGGC GCTCCTGCCA TGCCCGGCAT GGGTGGTATG 
GGCGGCATGG GAGGCATGGG CGGCATGGGT GGCATGATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511115.2
CDS	153..1874
Translation	MFRLPVSLARSSISRQLAMRGYAKDVRFGPEVRAMMLQGVDVLADAVAVTMGPKGRNVIIEQSWGSPKITKDGVTVAKSIELKDKFQNIGAKLVQDVANNTNEEAGDGTTTATVLARAIAKEGFEKISKGANPVEIRRGVMLAVETVKDNLKTMSRPVSTPEEIAQVATISANGDQAIGNLISEAMKKVGRDGVITVKDGKTLTDELEVIEGMKFDRGYISPYFINSSKGAKVEFQDALLLLSEKKISSVQSIIPALELANAQRKPLVIIAEDIDGEALSTLVVNRLKIGLQVAAVKAPGFGDNRKSTLTDMAIASGGIVFGDDADLVKLEDVKVSDLGQVGEVVITKDDTLLLKGKGKKDDVLRRANQIKDQIEDTTSEYEKEKLQERLARLASGVALLRVGGSSEVEVNEKKDRVHDALNATRAAVEEGIVPGGGTALLRCIEKLEGVETTNEDQKLGVEIVRRALRMPCMTIAKNAGVDGAMVVAKVENQAGDYGYDALKGEYGNLIEKGIIDPTKVVRTAITDASGVASLLTTAEAVVTEIPKEDGAPAMPGMGGMGGMGGMGGMGGMM

Target ORF information:

RefSeq Version	NM_078560.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heat shock protein 60A, transcript variant A (Hsp60A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078560.3

ORF Insert Sequence:

ATGTTCCGTT TGCCAGTTTC GCTTGCTCGC TCCTCCATTA GCCGCCAGTT GGCCATGCGC 
GGCTATGCCA AGGATGTGCG TTTCGGACCC GAGGTGCGCG CCATGATGCT CCAGGGCGTC 
GATGTGCTGG CCGATGCTGT GGCAGTGACC ATGGGCCCCA AGGGTCGCAA TGTGATCATT 
GAGCAGTCGT GGGGCTCGCC CAAAATCACC AAGGACGGCG TCACTGTGGC CAAGTCCATT 
GAGCTGAAGG ACAAGTTCCA GAACATCGGT GCCAAGCTGG TCCAGGATGT GGCCAACAAT 
ACCAACGAGG AGGCCGGTGA TGGCACCACC ACGGCCACCG TTCTGGCTCG CGCCATTGCC 
AAGGAGGGCT TCGAGAAGAT CTCCAAGGGC GCCAATCCCG TCGAGATTCG TCGCGGTGTC 
ATGCTGGCCG TTGAGACCGT CAAGGACAAC CTTAAGACCA TGTCGCGCCC CGTTAGCACG 
CCCGAGGAGA TCGCCCAGGT GGCCACCATC TCGGCCAACG GTGATCAGGC CATCGGCAAT 
CTCATCAGCG AGGCCATGAA GAAGGTGGGA CGCGATGGCG TTATCACCGT CAAGGATGGC 
AAGACCCTCA CCGATGAGTT GGAGGTTATC GAGGGCATGA AGTTCGATCG CGGCTACATC 
TCGCCGTACT TCATCAACTC CTCGAAGGGC GCCAAGGTGG AGTTCCAAGA TGCCCTCCTC 
CTGCTGTCCG AGAAGAAGAT CTCATCGGTG CAGAGCATCA TTCCCGCTCT GGAGCTGGCC 
AATGCGCAGC GCAAGCCGCT GGTCATCATT GCCGAGGATA TCGATGGTGA GGCCCTGAGC 
ACCCTGGTGG TGAACCGCCT CAAGATCGGC CTTCAGGTGG CTGCCGTCAA GGCACCCGGC 
TTCGGCGACA ACCGCAAGAG CACCCTCACC GATATGGCCA TCGCCTCCGG CGGCATTGTC 
TTCGGCGATG ATGCTGATCT CGTCAAGCTG GAGGACGTCA AGGTTAGCGA TTTGGGCCAG 
GTCGGCGAGG TGGTGATCAC CAAGGACGAC ACCCTGCTGC TGAAGGGCAA GGGCAAGAAG 
GACGATGTGC TCCGTCGCGC CAATCAGATC AAGGACCAGA TCGAGGACAC CACCTCCGAG 
TACGAGAAGG AGAAGCTGCA GGAGCGTCTG GCCCGCTTGG CCTCCGGCGT TGCCCTGCTG 
CGCGTTGGCG GCTCCAGCGA GGTGGAGGTC AACGAGAAGA AGGATCGCGT CCATGATGCC 
CTGAACGCCA CCCGTGCTGC CGTCGAAGAG GGAATCGTTC CCGGAGGCGG CACCGCTCTG 
CTGCGTTGCA TTGAGAAGCT CGAGGGTGTC GAGACCACCA ACGAGGATCA GAAACTCGGC 
GTGGAGATTG TGCGTCGTGC CCTGCGCATG CCATGCATGA CGATTGCCAA GAACGCCGGC 
GTTGATGGCG CCATGGTGGT GGCCAAGGTG GAGAATCAGG CCGGTGACTA CGGCTACGAT 
GCGCTGAAGG GCGAATACGG CAACCTGATC GAGAAGGGCA TCATCGATCC CACCAAGGTG 
GTGCGCACCG CCATCACAGA CGCCTCCGGT GTGGCCTCAC TGCTGACCAC CGCCGAGGCT 
GTCGTCACCG AGATCCCCAA GGAGGATGGC GCTCCTGCCA TGCCCGGCAT GGGTGGTATG 
GGCGGCATGG GAGGCATGGG CGGCATGGGT GGCATGATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsp60A ( NM_167266.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727489.1 Drosophila melanogaster heat shock protein 60A, transcript variant B (Hsp60A), mRNA.

Price & Availability↑

CloneID	OFa03472
Clone ID Related Accession (Same CDS sequence)	NM_078560.3 , NM_167266.1
Accession Version	NM_167266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	heat shock protein 60A, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCCGTT TGCCAGTTTC GCTTGCTCGC TCCTCCATTA GCCGCCAGTT GGCCATGCGC 
GGCTATGCCA AGGATGTGCG TTTCGGACCC GAGGTGCGCG CCATGATGCT CCAGGGCGTC 
GATGTGCTGG CCGATGCTGT GGCAGTGACC ATGGGCCCCA AGGGTCGCAA TGTGATCATT 
GAGCAGTCGT GGGGCTCGCC CAAAATCACC AAGGACGGCG TCACTGTGGC CAAGTCCATT 
GAGCTGAAGG ACAAGTTCCA GAACATCGGT GCCAAGCTGG TCCAGGATGT GGCCAACAAT 
ACCAACGAGG AGGCCGGTGA TGGCACCACC ACGGCCACCG TTCTGGCTCG CGCCATTGCC 
AAGGAGGGCT TCGAGAAGAT CTCCAAGGGC GCCAATCCCG TCGAGATTCG TCGCGGTGTC 
ATGCTGGCCG TTGAGACCGT CAAGGACAAC CTTAAGACCA TGTCGCGCCC CGTTAGCACG 
CCCGAGGAGA TCGCCCAGGT GGCCACCATC TCGGCCAACG GTGATCAGGC CATCGGCAAT 
CTCATCAGCG AGGCCATGAA GAAGGTGGGA CGCGATGGCG TTATCACCGT CAAGGATGGC 
AAGACCCTCA CCGATGAGTT GGAGGTTATC GAGGGCATGA AGTTCGATCG CGGCTACATC 
TCGCCGTACT TCATCAACTC CTCGAAGGGC GCCAAGGTGG AGTTCCAAGA TGCCCTCCTC 
CTGCTGTCCG AGAAGAAGAT CTCATCGGTG CAGAGCATCA TTCCCGCTCT GGAGCTGGCC 
AATGCGCAGC GCAAGCCGCT GGTCATCATT GCCGAGGATA TCGATGGTGA GGCCCTGAGC 
ACCCTGGTGG TGAACCGCCT CAAGATCGGC CTTCAGGTGG CTGCCGTCAA GGCACCCGGC 
TTCGGCGACA ACCGCAAGAG CACCCTCACC GATATGGCCA TCGCCTCCGG CGGCATTGTC 
TTCGGCGATG ATGCTGATCT CGTCAAGCTG GAGGACGTCA AGGTTAGCGA TTTGGGCCAG 
GTCGGCGAGG TGGTGATCAC CAAGGACGAC ACCCTGCTGC TGAAGGGCAA GGGCAAGAAG 
GACGATGTGC TCCGTCGCGC CAATCAGATC AAGGACCAGA TCGAGGACAC CACCTCCGAG 
TACGAGAAGG AGAAGCTGCA GGAGCGTCTG GCCCGCTTGG CCTCCGGCGT TGCCCTGCTG 
CGCGTTGGCG GCTCCAGCGA GGTGGAGGTC AACGAGAAGA AGGATCGCGT CCATGATGCC 
CTGAACGCCA CCCGTGCTGC CGTCGAAGAG GGAATCGTTC CCGGAGGCGG CACCGCTCTG 
CTGCGTTGCA TTGAGAAGCT CGAGGGTGTC GAGACCACCA ACGAGGATCA GAAACTCGGC 
GTGGAGATTG TGCGTCGTGC CCTGCGCATG CCATGCATGA CGATTGCCAA GAACGCCGGC 
GTTGATGGCG CCATGGTGGT GGCCAAGGTG GAGAATCAGG CCGGTGACTA CGGCTACGAT 
GCGCTGAAGG GCGAATACGG CAACCTGATC GAGAAGGGCA TCATCGATCC CACCAAGGTG 
GTGCGCACCG CCATCACAGA CGCCTCCGGT GTGGCCTCAC TGCTGACCAC CGCCGAGGCT 
GTCGTCACCG AGATCCCCAA GGAGGATGGC GCTCCTGCCA TGCCCGGCAT GGGTGGTATG 
GGCGGCATGG GAGGCATGGG CGGCATGGGT GGCATGATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727489.1
CDS	124..1845
Translation	MFRLPVSLARSSISRQLAMRGYAKDVRFGPEVRAMMLQGVDVLADAVAVTMGPKGRNVIIEQSWGSPKITKDGVTVAKSIELKDKFQNIGAKLVQDVANNTNEEAGDGTTTATVLARAIAKEGFEKISKGANPVEIRRGVMLAVETVKDNLKTMSRPVSTPEEIAQVATISANGDQAIGNLISEAMKKVGRDGVITVKDGKTLTDELEVIEGMKFDRGYISPYFINSSKGAKVEFQDALLLLSEKKISSVQSIIPALELANAQRKPLVIIAEDIDGEALSTLVVNRLKIGLQVAAVKAPGFGDNRKSTLTDMAIASGGIVFGDDADLVKLEDVKVSDLGQVGEVVITKDDTLLLKGKGKKDDVLRRANQIKDQIEDTTSEYEKEKLQERLARLASGVALLRVGGSSEVEVNEKKDRVHDALNATRAAVEEGIVPGGGTALLRCIEKLEGVETTNEDQKLGVEIVRRALRMPCMTIAKNAGVDGAMVVAKVENQAGDYGYDALKGEYGNLIEKGIIDPTKVVRTAITDASGVASLLTTAEAVVTEIPKEDGAPAMPGMGGMGGMGGMGGMGGMM

Target ORF information:

RefSeq Version	NM_167266.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster heat shock protein 60A, transcript variant B (Hsp60A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167266.1

ORF Insert Sequence:

ATGTTCCGTT TGCCAGTTTC GCTTGCTCGC TCCTCCATTA GCCGCCAGTT GGCCATGCGC 
GGCTATGCCA AGGATGTGCG TTTCGGACCC GAGGTGCGCG CCATGATGCT CCAGGGCGTC 
GATGTGCTGG CCGATGCTGT GGCAGTGACC ATGGGCCCCA AGGGTCGCAA TGTGATCATT 
GAGCAGTCGT GGGGCTCGCC CAAAATCACC AAGGACGGCG TCACTGTGGC CAAGTCCATT 
GAGCTGAAGG ACAAGTTCCA GAACATCGGT GCCAAGCTGG TCCAGGATGT GGCCAACAAT 
ACCAACGAGG AGGCCGGTGA TGGCACCACC ACGGCCACCG TTCTGGCTCG CGCCATTGCC 
AAGGAGGGCT TCGAGAAGAT CTCCAAGGGC GCCAATCCCG TCGAGATTCG TCGCGGTGTC 
ATGCTGGCCG TTGAGACCGT CAAGGACAAC CTTAAGACCA TGTCGCGCCC CGTTAGCACG 
CCCGAGGAGA TCGCCCAGGT GGCCACCATC TCGGCCAACG GTGATCAGGC CATCGGCAAT 
CTCATCAGCG AGGCCATGAA GAAGGTGGGA CGCGATGGCG TTATCACCGT CAAGGATGGC 
AAGACCCTCA CCGATGAGTT GGAGGTTATC GAGGGCATGA AGTTCGATCG CGGCTACATC 
TCGCCGTACT TCATCAACTC CTCGAAGGGC GCCAAGGTGG AGTTCCAAGA TGCCCTCCTC 
CTGCTGTCCG AGAAGAAGAT CTCATCGGTG CAGAGCATCA TTCCCGCTCT GGAGCTGGCC 
AATGCGCAGC GCAAGCCGCT GGTCATCATT GCCGAGGATA TCGATGGTGA GGCCCTGAGC 
ACCCTGGTGG TGAACCGCCT CAAGATCGGC CTTCAGGTGG CTGCCGTCAA GGCACCCGGC 
TTCGGCGACA ACCGCAAGAG CACCCTCACC GATATGGCCA TCGCCTCCGG CGGCATTGTC 
TTCGGCGATG ATGCTGATCT CGTCAAGCTG GAGGACGTCA AGGTTAGCGA TTTGGGCCAG 
GTCGGCGAGG TGGTGATCAC CAAGGACGAC ACCCTGCTGC TGAAGGGCAA GGGCAAGAAG 
GACGATGTGC TCCGTCGCGC CAATCAGATC AAGGACCAGA TCGAGGACAC CACCTCCGAG 
TACGAGAAGG AGAAGCTGCA GGAGCGTCTG GCCCGCTTGG CCTCCGGCGT TGCCCTGCTG 
CGCGTTGGCG GCTCCAGCGA GGTGGAGGTC AACGAGAAGA AGGATCGCGT CCATGATGCC 
CTGAACGCCA CCCGTGCTGC CGTCGAAGAG GGAATCGTTC CCGGAGGCGG CACCGCTCTG 
CTGCGTTGCA TTGAGAAGCT CGAGGGTGTC GAGACCACCA ACGAGGATCA GAAACTCGGC 
GTGGAGATTG TGCGTCGTGC CCTGCGCATG CCATGCATGA CGATTGCCAA GAACGCCGGC 
GTTGATGGCG CCATGGTGGT GGCCAAGGTG GAGAATCAGG CCGGTGACTA CGGCTACGAT 
GCGCTGAAGG GCGAATACGG CAACCTGATC GAGAAGGGCA TCATCGATCC CACCAAGGTG 
GTGCGCACCG CCATCACAGA CGCCTCCGGT GTGGCCTCAC TGCTGACCAC CGCCGAGGCT 
GTCGTCACCG AGATCCCCAA GGAGGATGGC GCTCCTGCCA TGCCCGGCAT GGGTGGTATG 
GGCGGCATGG GAGGCATGGG CGGCATGGGT GGCATGATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hsp60A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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