HSPD1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSPD1
Entrez Gene ID	511913
Full Name	heat shock protein family D (Hsp60) member 1
Synonyms	HSP60
General protein information	Preferred Names heat shock protein family D (Hsp60) member 1 Names 60 kDa heat shock protein, mitochondrial 60 kDa chaperonin CPN60 HSP-60 chaperonin 60 heat shock 60kDa protein 1 (chaperonin) heat shock protein 60 mitochondrial matrix protein P1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015458500.1	XP_015313986.1	60 kDa heat shock protein, mitochondrial isoform X1
NM_001166608.1	NP_001160080.1	60 kDa heat shock protein, mitochondrial
NM_001166610.1	NP_001160082.1	60 kDa heat shock protein, mitochondrial
NM_001166609.1	NP_001160081.1	60 kDa heat shock protein, mitochondrial

Pathways from BioSystems

Homologies

	HSPD1	XP_002799053.1
Danio rerio (zebrafish)	hspd1	NP_851847.1
Homo sapiens (human)	HSPD1	NP_955472.1
Gallus gallus (chicken)	HSPD1	NP_001012934.1
Saccharomyces cerevisiae (baker's yeast)	HSP60	NP_013360.1
Schizosaccharomyces pombe (fission yeast)	mcp60	NP_592894.1
Oryza sativa (rice)	Os10g0462900	NP_001064784.1
Pan troglodytes (chimpanzee)	HSPD1	XP_001169056.1
Mus musculus (house mouse)	Hspd1	NP_034607.3
Kluyveromyces lactis	KLLA0F09449g	XP_455510.1
Magnaporthe oryzae (rice blast fungus)	MGG_03165	XP_003716826.1
Arabidopsis thaliana (thale cress)	HSP60	NP_189041.1
Oryza sativa (rice)	Os03g0143400	NP_001048938.1
Canis lupus familiaris (dog)	HSPD1	XP_005640555.1
Bos taurus (cattle)	HSPD1	NP_001160082.1
Drosophila melanogaster (fruit fly)	Hsp60	NP_511115.2
Caenorhabditis elegans (roundworm)	hsp-60	NP_497429.1
Rattus norvegicus (Norway rat)	Hspd1	NP_071565.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004002	XP_318461.2
Eremothecium gossypii	AGOS_AFR155W	NP_985702.2
Neurospora crassa	NCU01589	XP_956500.2
Xenopus tropicalis (tropical clawed frog)	hspd1	NP_001096355.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of Heat Shock Protein 60 as a Regulator of Neutral Sphingomyelinase 2 and Its Role in Dopamine Uptake.
Ahn KH, Kim SK, Choi JM, Jung SY, Won JH, Back MJ, Fu Z, Jang JM, Ha HC, Kim DK
PloS one8(6)e67216(2013)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Localization of the chaperone proteins GRP78 and HSP60 on the luminal surface of bovine oviduct epithelial cells and their association with spermatozoa.
Boilard M, Reyes-Moreno C, Lachance C, Massicotte L, Bailey JL, Sirard MA, Leclerc P
Biology of reproduction71(6)1879-89(2004 Dec)

Molecular mimicry in Lyme disease: monoclonal antibody H9724 to B. burgdorferi flagellin specifically detects chaperonin-HSP60.
Dai Z, Lackland H, Stein S, Li Q, Radziewicz R, Williams S, Sigal LH
Biochimica et biophysica acta1181(1)97-100(1993 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This paper reports the localization of both GRP78 and HSP60 on the luminal/apical surface of oviduct epithelial cells, their binding to spermatozoa, and the presence of endogenous HSP60 in the sperm midpiece.
Title: Localization of the chaperone proteins GRP78 and HSP60 on the luminal surface of bovine oviduct epithelial cells and their association with spermatozoa.

The following HSPD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSPD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01575	NM_001166610.1 Latest version!	Bos taurus heat shock protein family D (Hsp60) member 1 (HSPD1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa01575	NM_001166608.1 Latest version!	Bos taurus heat shock 60kDa protein 1 (chaperonin) (HSPD1), nuclear gene encoding mitochondrial protein, transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa01575	NM_001166609.1 Latest version!	Bos taurus heat shock protein family D (Hsp60) member 1 (HSPD1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa87963	XM_015458500.1 Latest version!	Bos taurus heat shock protein family D (Hsp60) member 1 (HSPD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSPD1 ( NM_001166608.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001160080.1 Bos taurus heat shock 60kDa protein 1 (chaperonin) (HSPD1), nuclear gene encoding mitochondrial protein, transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa01575
Clone ID Related Accession (Same CDS sequence)	NM_001166608.1 , NM_001166610.1 , NM_001166609.1
Accession Version	NM_001166608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2010-02-13
Organism	Bos taurus(cattle)
Product	60 kDa heat shock protein, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03048492.1. On Oct 30, 2009 this sequence version replaced XM_001249790.2. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. COMPLETENESS: complete on the 3' end.

ATGCTTCGAT TACCCGCAGT CCTTCGTCAA ATGAGGCCAG TGTCCAGGGC ACTGGCTCCT 
CATCTCACTC GGGCTTATGC CAAAGATGTA AAATTCGGTG CAGATGCTCG AGCCTTAATG 
CTTCAAGGTG TAGACCTTTT AGCCGATGCT GTAGCCGTTA CTATGGGGCC AAAGGGAAGG 
ACAGTGATTA TTGAACAGAG TTGGGGAAGT CCCAAAGTGA CAAAAGATGG TGTGACTGTT 
GCAAAGTCTA TTGATTTAAA AGATAAATAT AAAAATATTG GCGCTAAACT TGTTCAAGAT 
GTTGCCAATA ACACAAATGA AGAGGCGGGG GATGGCACCA CTACTGCTAC TGTACTGGCA 
CGCTCTATTG CCAAGGAAGG CTTCGAGAAG ATTAGCAAAG GTGCTAATCC AGTGGAAATC 
AGGAGAGGTG TGATGTTAGC TGTTGATGCT GTAATTGCTG AACTTAAGAA GCAGTCTAAA 
CCTGTGACAA CCCCCGAAGA GATTGCTCAG GTTGCTACAA TTTCTGCAAA TGGAGACAAA 
GAAATTGGCA ACATCATTTC TGATGCCATG AAAAAAGTTG GAAGAAAGGG TGTTATCACA 
GTAAAGGATG GAAAAACACT GAATGATGAA TTAGAAATTA TTGAAGGCAT GAAGTTTGAC 
AGAGGATATA TTTCTCCATA CTTTATTAAT ACATCAAAAG GTCAGAAATG TGAATTCCAA 
GATGCATATG TTCTATTGAG TGAAAAGAAA ATTTCTAGTG TCCAATCCAT TGTTCCTGCT 
CTTGAAATTG CCAATGCTCA CCGGAAGCCC TTGGTCATAA TTGCTGAAGA TGTGGATGGA 
GAAGCTCTAA GTACCCTTGT TTTGAATAGG TTGAAGGTTG GTCTTCAAGT TGTGGCAGTC 
AAAGCTCCAG GTTTTGGTGA CAATAGAAAG AACCAGCTTA AAGACATGGC TATTGCTACT 
GGGGGTGCAG TATTTGGAGA AGAAGGACTA AATCTAAATC TTGAAGATGT TCAGCCTCAT 
GACTTAGGAA AAGTTGGAGA GGTCATTGTG ACCAAAGATG ATGCCATGCT CTTGAAAGGA 
AAAGGTGACA AGGCTCAAAT TGAGAAGCGT ATCCAAGAGA TCATTGAGCA GTTAGATATC 
ACAACTAGTG AATATGAAAA GGAAAAACTG AATGAACGTC TGGCAAAACT CTCAGATGGT 
GTTGCTGTGT TGAAGGTTGG TGGGACAAGT GATGTTGAAG TGAATGAAAA GAAAGACAGA 
GTTACAGATG CTCTTAATGC CACACGAGCT GCTGTTGAAG AAGGCATTGT TCTGGGAGGG 
GGCTGTGCCC TGCTTCGGTG CATTCCAGCC TTGGAGTCAA TAACTCCAGC TAATGAAGAT 
CAAAAAACAG GTATAGAAAT AATTAAAAAA ACACTCAAAA TTCCTGCAAT GACCATTGCT 
AAGAATGCAG GTGTTGAAGG ATCATTGATA GTTGAGAAAA TTATGCAGAG TTCTTCAGAA 
GTCGGTTATG ATGCTATGCT TGGAGATTTT GTGAATATGG TGGAAAAAGG AATCATTGAT 
CCAACTAAGG TTGTAAGAAC TGCATTACTG GATGCTGCCG GAGTGGCCTC TCTCTTAACA 
ACAGCAGAAG TTGTCGTCAC AGAAATTCCT AAAGAAGAGA AGGATCCTGG AATGGGTGGC 
ATGGGTGGAA TGGGAGGTGG CATGGGAGGT GGCATGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001160080.1
CDS	360..2081
Translation	MLRLPAVLRQMRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSVQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLNLNLEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDITTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALESITPANEDQKTGIEIIKKTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEVGYDAMLGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGGMGGMGGGMGGGMF

Target ORF information:

RefSeq Version	NM_001166608.1
Organism	Bos taurus(cattle)
Definition	Bos taurus heat shock 60kDa protein 1 (chaperonin) (HSPD1), nuclear gene encoding mitochondrial protein, transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166608.1

ORF Insert Sequence:

ATGCTTCGAT TACCCGCAGT CCTTCGTCAA ATGAGGCCAG TGTCCAGGGC ACTGGCTCCT 
CATCTCACTC GGGCTTATGC CAAAGATGTA AAATTCGGTG CAGATGCTCG AGCCTTAATG 
CTTCAAGGTG TAGACCTTTT AGCCGATGCT GTAGCCGTTA CTATGGGGCC AAAGGGAAGG 
ACAGTGATTA TTGAACAGAG TTGGGGAAGT CCCAAAGTGA CAAAAGATGG TGTGACTGTT 
GCAAAGTCTA TTGATTTAAA AGATAAATAT AAAAATATTG GCGCTAAACT TGTTCAAGAT 
GTTGCCAATA ACACAAATGA AGAGGCGGGG GATGGCACCA CTACTGCTAC TGTACTGGCA 
CGCTCTATTG CCAAGGAAGG CTTCGAGAAG ATTAGCAAAG GTGCTAATCC AGTGGAAATC 
AGGAGAGGTG TGATGTTAGC TGTTGATGCT GTAATTGCTG AACTTAAGAA GCAGTCTAAA 
CCTGTGACAA CCCCCGAAGA GATTGCTCAG GTTGCTACAA TTTCTGCAAA TGGAGACAAA 
GAAATTGGCA ACATCATTTC TGATGCCATG AAAAAAGTTG GAAGAAAGGG TGTTATCACA 
GTAAAGGATG GAAAAACACT GAATGATGAA TTAGAAATTA TTGAAGGCAT GAAGTTTGAC 
AGAGGATATA TTTCTCCATA CTTTATTAAT ACATCAAAAG GTCAGAAATG TGAATTCCAA 
GATGCATATG TTCTATTGAG TGAAAAGAAA ATTTCTAGTG TCCAATCCAT TGTTCCTGCT 
CTTGAAATTG CCAATGCTCA CCGGAAGCCC TTGGTCATAA TTGCTGAAGA TGTGGATGGA 
GAAGCTCTAA GTACCCTTGT TTTGAATAGG TTGAAGGTTG GTCTTCAAGT TGTGGCAGTC 
AAAGCTCCAG GTTTTGGTGA CAATAGAAAG AACCAGCTTA AAGACATGGC TATTGCTACT 
GGGGGTGCAG TATTTGGAGA AGAAGGACTA AATCTAAATC TTGAAGATGT TCAGCCTCAT 
GACTTAGGAA AAGTTGGAGA GGTCATTGTG ACCAAAGATG ATGCCATGCT CTTGAAAGGA 
AAAGGTGACA AGGCTCAAAT TGAGAAGCGT ATCCAAGAGA TCATTGAGCA GTTAGATATC 
ACAACTAGTG AATATGAAAA GGAAAAACTG AATGAACGTC TGGCAAAACT CTCAGATGGT 
GTTGCTGTGT TGAAGGTTGG TGGGACAAGT GATGTTGAAG TGAATGAAAA GAAAGACAGA 
GTTACAGATG CTCTTAATGC CACACGAGCT GCTGTTGAAG AAGGCATTGT TCTGGGAGGG 
GGCTGTGCCC TGCTTCGGTG CATTCCAGCC TTGGAGTCAA TAACTCCAGC TAATGAAGAT 
CAAAAAACAG GTATAGAAAT AATTAAAAAA ACACTCAAAA TTCCTGCAAT GACCATTGCT 
AAGAATGCAG GTGTTGAAGG ATCATTGATA GTTGAGAAAA TTATGCAGAG TTCTTCAGAA 
GTCGGTTATG ATGCTATGCT TGGAGATTTT GTGAATATGG TGGAAAAAGG AATCATTGAT 
CCAACTAAGG TTGTAAGAAC TGCATTACTG GATGCTGCCG GAGTGGCCTC TCTCTTAACA 
ACAGCAGAAG TTGTCGTCAC AGAAATTCCT AAAGAAGAGA AGGATCCTGG AATGGGTGGC 
ATGGGTGGAA TGGGAGGTGG CATGGGAGGT GGCATGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPD1 ( NM_001166610.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001160082.1 Bos taurus heat shock protein family D (Hsp60) member 1 (HSPD1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OBa01575
Clone ID Related Accession (Same CDS sequence)	NM_001166608.1 , NM_001166610.1 , NM_001166609.1
Accession Version	NM_001166610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Bos taurus(cattle)
Product	60 kDa heat shock protein, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000002.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571263.8477.1, SRR5571254.4186.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN04335745, SAMN05861761 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTTCGAT TACCCGCAGT CCTTCGTCAA ATGAGGCCAG TGTCCAGGGC ACTGGCTCCT 
CATCTCACTC GGGCTTATGC CAAAGATGTA AAATTCGGTG CAGATGCTCG AGCCTTAATG 
CTTCAAGGTG TAGACCTTTT AGCCGATGCT GTAGCCGTTA CTATGGGGCC AAAGGGAAGG 
ACAGTGATTA TTGAACAGAG TTGGGGAAGT CCCAAAGTGA CAAAAGATGG TGTGACTGTT 
GCAAAGTCTA TTGATTTAAA AGATAAATAT AAAAATATTG GCGCTAAACT TGTTCAAGAT 
GTTGCCAATA ACACAAATGA AGAGGCGGGG GATGGCACCA CTACTGCTAC TGTACTGGCA 
CGCTCTATTG CCAAGGAAGG CTTCGAGAAG ATTAGCAAAG GTGCTAATCC AGTGGAAATC 
AGGAGAGGTG TGATGTTAGC TGTTGATGCT GTAATTGCTG AACTTAAGAA GCAGTCTAAA 
CCTGTGACAA CCCCCGAAGA GATTGCTCAG GTTGCTACAA TTTCTGCAAA TGGAGACAAA 
GAAATTGGCA ACATCATTTC TGATGCCATG AAAAAAGTTG GAAGAAAGGG TGTTATCACA 
GTAAAGGATG GAAAAACACT GAATGATGAA TTAGAAATTA TTGAAGGCAT GAAGTTTGAC 
AGAGGATATA TTTCTCCATA CTTTATTAAT ACATCAAAAG GTCAGAAATG TGAATTCCAA 
GATGCATATG TTCTATTGAG TGAAAAGAAA ATTTCTAGTG TCCAATCCAT TGTTCCTGCT 
CTTGAAATTG CCAATGCTCA CCGGAAGCCC TTGGTCATAA TTGCTGAAGA TGTGGATGGA 
GAAGCTCTAA GTACCCTTGT TTTGAATAGG TTGAAGGTTG GTCTTCAAGT TGTGGCAGTC 
AAAGCTCCAG GTTTTGGTGA CAATAGAAAG AACCAGCTTA AAGACATGGC TATTGCTACT 
GGGGGTGCAG TATTTGGAGA AGAAGGACTA AATCTAAATC TTGAAGATGT TCAGCCTCAT 
GACTTAGGAA AAGTTGGAGA GGTCATTGTG ACCAAAGATG ATGCCATGCT CTTGAAAGGA 
AAAGGTGACA AGGCTCAAAT TGAGAAGCGT ATCCAAGAGA TCATTGAGCA GTTAGATATC 
ACAACTAGTG AATATGAAAA GGAAAAACTG AATGAACGTC TGGCAAAACT CTCAGATGGT 
GTTGCTGTGT TGAAGGTTGG TGGGACAAGT GATGTTGAAG TGAATGAAAA GAAAGACAGA 
GTTACAGATG CTCTTAATGC CACACGAGCT GCTGTTGAAG AAGGCATTGT TCTGGGAGGG 
GGCTGTGCCC TGCTTCGGTG CATTCCAGCC TTGGAGTCAA TAACTCCAGC TAATGAAGAT 
CAAAAAACAG GTATAGAAAT AATTAAAAAA ACACTCAAAA TTCCTGCAAT GACCATTGCT 
AAGAATGCAG GTGTTGAAGG ATCATTGATA GTTGAGAAAA TTATGCAGAG TTCTTCAGAA 
GTCGGTTATG ATGCTATGCT TGGAGATTTT GTGAATATGG TGGAAAAAGG AATCATTGAT 
CCAACTAAGG TTGTAAGAAC TGCATTACTG GATGCTGCCG GAGTGGCCTC TCTCTTAACA 
ACAGCAGAAG TTGTCGTCAC AGAAATTCCT AAAGAAGAGA AGGATCCTGG AATGGGTGGC 
ATGGGTGGAA TGGGAGGTGG CATGGGAGGT GGCATGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001160082.1
CDS	112..1833
Translation	MLRLPAVLRQMRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSVQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLNLNLEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDITTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALESITPANEDQKTGIEIIKKTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEVGYDAMLGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGGMGGMGGGMGGGMF

Target ORF information:

RefSeq Version	NM_001166610.1
Organism	Bos taurus(cattle)
Definition	Bos taurus heat shock protein family D (Hsp60) member 1 (HSPD1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166610.1

ORF Insert Sequence:

ATGCTTCGAT TACCCGCAGT CCTTCGTCAA ATGAGGCCAG TGTCCAGGGC ACTGGCTCCT 
CATCTCACTC GGGCTTATGC CAAAGATGTA AAATTCGGTG CAGATGCTCG AGCCTTAATG 
CTTCAAGGTG TAGACCTTTT AGCCGATGCT GTAGCCGTTA CTATGGGGCC AAAGGGAAGG 
ACAGTGATTA TTGAACAGAG TTGGGGAAGT CCCAAAGTGA CAAAAGATGG TGTGACTGTT 
GCAAAGTCTA TTGATTTAAA AGATAAATAT AAAAATATTG GCGCTAAACT TGTTCAAGAT 
GTTGCCAATA ACACAAATGA AGAGGCGGGG GATGGCACCA CTACTGCTAC TGTACTGGCA 
CGCTCTATTG CCAAGGAAGG CTTCGAGAAG ATTAGCAAAG GTGCTAATCC AGTGGAAATC 
AGGAGAGGTG TGATGTTAGC TGTTGATGCT GTAATTGCTG AACTTAAGAA GCAGTCTAAA 
CCTGTGACAA CCCCCGAAGA GATTGCTCAG GTTGCTACAA TTTCTGCAAA TGGAGACAAA 
GAAATTGGCA ACATCATTTC TGATGCCATG AAAAAAGTTG GAAGAAAGGG TGTTATCACA 
GTAAAGGATG GAAAAACACT GAATGATGAA TTAGAAATTA TTGAAGGCAT GAAGTTTGAC 
AGAGGATATA TTTCTCCATA CTTTATTAAT ACATCAAAAG GTCAGAAATG TGAATTCCAA 
GATGCATATG TTCTATTGAG TGAAAAGAAA ATTTCTAGTG TCCAATCCAT TGTTCCTGCT 
CTTGAAATTG CCAATGCTCA CCGGAAGCCC TTGGTCATAA TTGCTGAAGA TGTGGATGGA 
GAAGCTCTAA GTACCCTTGT TTTGAATAGG TTGAAGGTTG GTCTTCAAGT TGTGGCAGTC 
AAAGCTCCAG GTTTTGGTGA CAATAGAAAG AACCAGCTTA AAGACATGGC TATTGCTACT 
GGGGGTGCAG TATTTGGAGA AGAAGGACTA AATCTAAATC TTGAAGATGT TCAGCCTCAT 
GACTTAGGAA AAGTTGGAGA GGTCATTGTG ACCAAAGATG ATGCCATGCT CTTGAAAGGA 
AAAGGTGACA AGGCTCAAAT TGAGAAGCGT ATCCAAGAGA TCATTGAGCA GTTAGATATC 
ACAACTAGTG AATATGAAAA GGAAAAACTG AATGAACGTC TGGCAAAACT CTCAGATGGT 
GTTGCTGTGT TGAAGGTTGG TGGGACAAGT GATGTTGAAG TGAATGAAAA GAAAGACAGA 
GTTACAGATG CTCTTAATGC CACACGAGCT GCTGTTGAAG AAGGCATTGT TCTGGGAGGG 
GGCTGTGCCC TGCTTCGGTG CATTCCAGCC TTGGAGTCAA TAACTCCAGC TAATGAAGAT 
CAAAAAACAG GTATAGAAAT AATTAAAAAA ACACTCAAAA TTCCTGCAAT GACCATTGCT 
AAGAATGCAG GTGTTGAAGG ATCATTGATA GTTGAGAAAA TTATGCAGAG TTCTTCAGAA 
GTCGGTTATG ATGCTATGCT TGGAGATTTT GTGAATATGG TGGAAAAAGG AATCATTGAT 
CCAACTAAGG TTGTAAGAAC TGCATTACTG GATGCTGCCG GAGTGGCCTC TCTCTTAACA 
ACAGCAGAAG TTGTCGTCAC AGAAATTCCT AAAGAAGAGA AGGATCCTGG AATGGGTGGC 
ATGGGTGGAA TGGGAGGTGG CATGGGAGGT GGCATGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPD1 ( NM_001166609.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001160081.1 Bos taurus heat shock protein family D (Hsp60) member 1 (HSPD1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OBa01575
Clone ID Related Accession (Same CDS sequence)	NM_001166608.1 , NM_001166610.1 , NM_001166609.1
Accession Version	NM_001166609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Bos taurus(cattle)
Product	60 kDa heat shock protein, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000002.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571254.36653.1, SRR6764281.8526.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145467 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTTCGAT TACCCGCAGT CCTTCGTCAA ATGAGGCCAG TGTCCAGGGC ACTGGCTCCT 
CATCTCACTC GGGCTTATGC CAAAGATGTA AAATTCGGTG CAGATGCTCG AGCCTTAATG 
CTTCAAGGTG TAGACCTTTT AGCCGATGCT GTAGCCGTTA CTATGGGGCC AAAGGGAAGG 
ACAGTGATTA TTGAACAGAG TTGGGGAAGT CCCAAAGTGA CAAAAGATGG TGTGACTGTT 
GCAAAGTCTA TTGATTTAAA AGATAAATAT AAAAATATTG GCGCTAAACT TGTTCAAGAT 
GTTGCCAATA ACACAAATGA AGAGGCGGGG GATGGCACCA CTACTGCTAC TGTACTGGCA 
CGCTCTATTG CCAAGGAAGG CTTCGAGAAG ATTAGCAAAG GTGCTAATCC AGTGGAAATC 
AGGAGAGGTG TGATGTTAGC TGTTGATGCT GTAATTGCTG AACTTAAGAA GCAGTCTAAA 
CCTGTGACAA CCCCCGAAGA GATTGCTCAG GTTGCTACAA TTTCTGCAAA TGGAGACAAA 
GAAATTGGCA ACATCATTTC TGATGCCATG AAAAAAGTTG GAAGAAAGGG TGTTATCACA 
GTAAAGGATG GAAAAACACT GAATGATGAA TTAGAAATTA TTGAAGGCAT GAAGTTTGAC 
AGAGGATATA TTTCTCCATA CTTTATTAAT ACATCAAAAG GTCAGAAATG TGAATTCCAA 
GATGCATATG TTCTATTGAG TGAAAAGAAA ATTTCTAGTG TCCAATCCAT TGTTCCTGCT 
CTTGAAATTG CCAATGCTCA CCGGAAGCCC TTGGTCATAA TTGCTGAAGA TGTGGATGGA 
GAAGCTCTAA GTACCCTTGT TTTGAATAGG TTGAAGGTTG GTCTTCAAGT TGTGGCAGTC 
AAAGCTCCAG GTTTTGGTGA CAATAGAAAG AACCAGCTTA AAGACATGGC TATTGCTACT 
GGGGGTGCAG TATTTGGAGA AGAAGGACTA AATCTAAATC TTGAAGATGT TCAGCCTCAT 
GACTTAGGAA AAGTTGGAGA GGTCATTGTG ACCAAAGATG ATGCCATGCT CTTGAAAGGA 
AAAGGTGACA AGGCTCAAAT TGAGAAGCGT ATCCAAGAGA TCATTGAGCA GTTAGATATC 
ACAACTAGTG AATATGAAAA GGAAAAACTG AATGAACGTC TGGCAAAACT CTCAGATGGT 
GTTGCTGTGT TGAAGGTTGG TGGGACAAGT GATGTTGAAG TGAATGAAAA GAAAGACAGA 
GTTACAGATG CTCTTAATGC CACACGAGCT GCTGTTGAAG AAGGCATTGT TCTGGGAGGG 
GGCTGTGCCC TGCTTCGGTG CATTCCAGCC TTGGAGTCAA TAACTCCAGC TAATGAAGAT 
CAAAAAACAG GTATAGAAAT AATTAAAAAA ACACTCAAAA TTCCTGCAAT GACCATTGCT 
AAGAATGCAG GTGTTGAAGG ATCATTGATA GTTGAGAAAA TTATGCAGAG TTCTTCAGAA 
GTCGGTTATG ATGCTATGCT TGGAGATTTT GTGAATATGG TGGAAAAAGG AATCATTGAT 
CCAACTAAGG TTGTAAGAAC TGCATTACTG GATGCTGCCG GAGTGGCCTC TCTCTTAACA 
ACAGCAGAAG TTGTCGTCAC AGAAATTCCT AAAGAAGAGA AGGATCCTGG AATGGGTGGC 
ATGGGTGGAA TGGGAGGTGG CATGGGAGGT GGCATGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001160081.1
CDS	245..1966
Translation	MLRLPAVLRQMRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSVQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLNLNLEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDITTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALESITPANEDQKTGIEIIKKTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEVGYDAMLGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGGMGGMGGGMGGGMF

Target ORF information:

RefSeq Version	NM_001166609.1
Organism	Bos taurus(cattle)
Definition	Bos taurus heat shock protein family D (Hsp60) member 1 (HSPD1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166609.1

ORF Insert Sequence:

ATGCTTCGAT TACCCGCAGT CCTTCGTCAA ATGAGGCCAG TGTCCAGGGC ACTGGCTCCT 
CATCTCACTC GGGCTTATGC CAAAGATGTA AAATTCGGTG CAGATGCTCG AGCCTTAATG 
CTTCAAGGTG TAGACCTTTT AGCCGATGCT GTAGCCGTTA CTATGGGGCC AAAGGGAAGG 
ACAGTGATTA TTGAACAGAG TTGGGGAAGT CCCAAAGTGA CAAAAGATGG TGTGACTGTT 
GCAAAGTCTA TTGATTTAAA AGATAAATAT AAAAATATTG GCGCTAAACT TGTTCAAGAT 
GTTGCCAATA ACACAAATGA AGAGGCGGGG GATGGCACCA CTACTGCTAC TGTACTGGCA 
CGCTCTATTG CCAAGGAAGG CTTCGAGAAG ATTAGCAAAG GTGCTAATCC AGTGGAAATC 
AGGAGAGGTG TGATGTTAGC TGTTGATGCT GTAATTGCTG AACTTAAGAA GCAGTCTAAA 
CCTGTGACAA CCCCCGAAGA GATTGCTCAG GTTGCTACAA TTTCTGCAAA TGGAGACAAA 
GAAATTGGCA ACATCATTTC TGATGCCATG AAAAAAGTTG GAAGAAAGGG TGTTATCACA 
GTAAAGGATG GAAAAACACT GAATGATGAA TTAGAAATTA TTGAAGGCAT GAAGTTTGAC 
AGAGGATATA TTTCTCCATA CTTTATTAAT ACATCAAAAG GTCAGAAATG TGAATTCCAA 
GATGCATATG TTCTATTGAG TGAAAAGAAA ATTTCTAGTG TCCAATCCAT TGTTCCTGCT 
CTTGAAATTG CCAATGCTCA CCGGAAGCCC TTGGTCATAA TTGCTGAAGA TGTGGATGGA 
GAAGCTCTAA GTACCCTTGT TTTGAATAGG TTGAAGGTTG GTCTTCAAGT TGTGGCAGTC 
AAAGCTCCAG GTTTTGGTGA CAATAGAAAG AACCAGCTTA AAGACATGGC TATTGCTACT 
GGGGGTGCAG TATTTGGAGA AGAAGGACTA AATCTAAATC TTGAAGATGT TCAGCCTCAT 
GACTTAGGAA AAGTTGGAGA GGTCATTGTG ACCAAAGATG ATGCCATGCT CTTGAAAGGA 
AAAGGTGACA AGGCTCAAAT TGAGAAGCGT ATCCAAGAGA TCATTGAGCA GTTAGATATC 
ACAACTAGTG AATATGAAAA GGAAAAACTG AATGAACGTC TGGCAAAACT CTCAGATGGT 
GTTGCTGTGT TGAAGGTTGG TGGGACAAGT GATGTTGAAG TGAATGAAAA GAAAGACAGA 
GTTACAGATG CTCTTAATGC CACACGAGCT GCTGTTGAAG AAGGCATTGT TCTGGGAGGG 
GGCTGTGCCC TGCTTCGGTG CATTCCAGCC TTGGAGTCAA TAACTCCAGC TAATGAAGAT 
CAAAAAACAG GTATAGAAAT AATTAAAAAA ACACTCAAAA TTCCTGCAAT GACCATTGCT 
AAGAATGCAG GTGTTGAAGG ATCATTGATA GTTGAGAAAA TTATGCAGAG TTCTTCAGAA 
GTCGGTTATG ATGCTATGCT TGGAGATTTT GTGAATATGG TGGAAAAAGG AATCATTGAT 
CCAACTAAGG TTGTAAGAAC TGCATTACTG GATGCTGCCG GAGTGGCCTC TCTCTTAACA 
ACAGCAGAAG TTGTCGTCAC AGAAATTCCT AAAGAAGAGA AGGATCCTGG AATGGGTGGC 
ATGGGTGGAA TGGGAGGTGG CATGGGAGGT GGCATGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPD1 ( XM_015458500.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015313986.1 Bos taurus heat shock protein family D (Hsp60) member 1 (HSPD1), transcript variant X1, mRNA.

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CloneID	OBa87963
Clone ID Related Accession (Same CDS sequence)	XM_015458500.1
Accession Version	XM_015458500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	60 kDa heat shock protein, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCGTT TGACCCTTGA CCCACATAGA GCCAGGCTCT TTCCGGAACG TTCTGGAACG 
GCGCGCAGCC CGGGAACTAG CCTAAGCGAG CCGGAGAGGG CTGAACCCGC TGGCCTGGCC 
CGGCGCGGGT ACGCGCCCGC TCCGCGCCGC CTCACCCGTG CGTTTCGGAC TCCGGAGCCG 
TCCTTCCCCT CTCCGTTGAG CCGGTCCGCC GGCGAGAGCC AGAGCTTCCC CCGGCGCCGC 
AGGCCCGGGC CCGCTGAGCC AGGCCTCGTG GGTGCACGCG GGGGCCCGTC ACGTGCGGCG 
TGGATCGCTC GCCCAGCCGG GCACAGCAGG GAAGGCTTGC CCACGAGTGT GCGCGGAGTC 
GGGCGATTGT ATTCACATCG ATCCTCCGAA ATGCTTCGAT TACCCGCAGT CCTTCGTCAA 
ATGAGGCCAG TGTCCAGGGC ACTGGCTCCT CATCTCACTC GGGCTTATGC CAAAGATGTA 
AAATTCGGTG CAGATGCTCG AGCCTTAATG CTTCAAGGTG TAGACCTTTT AGCCGATGCT 
GTAGCCGTTA CTATGGGGCC AAAGGGAAGG ACAGTGATTA TTGAACAGAG TTGGGGAAGT 
CCCAAAGTGA CAAAAGATGG TGTGACTGTT GCAAAGTCTA TTGATTTAAA AGATAAATAT 
AAAAATATTG GCGCTAAACT TGTTCAAGAT GTTGCCAATA ACACAAATGA AGAGGCGGGG 
GATGGCACCA CTACTGCTAC TGTACTGGCA CGCTCTATTG CCAAGGAAGG CTTCGAGAAG 
ATTAGCAAAG GTGCTAATCC AGTGGAAATC AGGAGAGGTG TGATGTTAGC TGTTGATGCT 
GTAATTGCTG AACTTAAGAA GCAGTCTAAA CCTGTGACAA CCCCCGAAGA GATTGCTCAG 
GTTGCTACAA TTTCTGCAAA TGGAGACAAA GAAATTGGCA ACATCATTTC TGATGCCATG 
AAAAAAGTTG GAAGAAAGGG TGTTATCACA GTAAAGGATG GAAAAACACT GAATGATGAA 
TTAGAAATTA TTGAAGGCAT GAAGTTTGAC AGAGGATATA TTTCTCCATA CTTTATTAAT 
ACATCAAAAG GTCAGAAATG TGAATTCCAA GATGCATATG TTCTATTGAG TGAAAAGAAA 
ATTTCTAGTG TCCAATCCAT TGTTCCTGCT CTTGAAATTG CCAATGCTCA CCGGAAGCCC 
TTGGTCATAA TTGCTGAAGA TGTGGATGGA GAAGCTCTAA GTACCCTTGT TTTGAATAGG 
TTGAAGGTTG GTCTTCAAGT TGTGGCAGTC AAAGCTCCAG GTTTTGGTGA CAATAGAAAG 
AACCAGCTTA AAGACATGGC TATTGCTACT GGGGGTGCAG TATTTGGAGA AGAAGGACTA 
AATCTAAATC TTGAAGATGT TCAGCCTCAT GACTTAGGAA AAGTTGGAGA GGTCATTGTG 
ACCAAAGATG ATGCCATGCT CTTGAAAGGA AAAGGTGACA AGGCTCAAAT TGAGAAGCGT 
ATCCAAGAGA TCATTGAGCA GTTAGATATC ACAACTAGTG AATATGAAAA GGAAAAACTG 
AATGAACGTC TGGCAAAACT CTCAGATGGT GTTGCTGTGT TGAAGGTTGG TGGGACAAGT 
GATGTTGAAG TGAATGAAAA GAAAGACAGA GTTACAGATG CTCTTAATGC CACACGAGCT 
GCTGTTGAAG AAGGCATTGT TCTGGGAGGG GGCTGTGCCC TGCTTCGGTG CATTCCAGCC 
TTGGAGTCAA TAACTCCAGC TAATGAAGAT CAAAAAACAG GTATAGAAAT AATTAAAAAA 
ACACTCAAAA TTCCTGCAAT GACCATTGCT AAGAATGCAG GTGTTGAAGG ATCATTGATA 
GTTGAGAAAA TTATGCAGAG TTCTTCAGAA GTCGGTTATG ATGCTATGCT TGGAGATTTT 
GTGAATATGG TGGAAAAAGG AATCATTGAT CCAACTAAGG TTGTAAGAAC TGCATTACTG 
GATGCTGCCG GAGTGGCCTC TCTCTTAACA ACAGCAGAAG TTGTCGTCAC AGAAATTCCT 
AAAGAAGAGA AGGATCCTGG AATGGGTGGC ATGGGTGGAA TGGGAGGTGG CATGGGAGGT 
GGCATGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015313986.1
CDS	108..2219
Translation	MTRLTLDPHRARLFPERSGTARSPGTSLSEPERAEPAGLARRGYAPAPRRLTRAFRTPEPSFPSPLSRSAGESQSFPRRRRPGPAEPGLVGARGGPSRAAWIARPAGHSREGLPTSVRGVGRLYSHRSSEMLRLPAVLRQMRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSVQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLNLNLEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDITTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALESITPANEDQKTGIEIIKKTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEVGYDAMLGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGGMGGMGGGMGGGMF

Target ORF information:

RefSeq Version	XM_015458500.1
Organism	Bos taurus(cattle)
Definition	Bos taurus heat shock protein family D (Hsp60) member 1 (HSPD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458500.1

ORF Insert Sequence:

ATGACGCGTT TGACCCTTGA CCCACATAGA GCCAGGCTCT TTCCGGAACG TTCTGGAACG 
GCGCGCAGCC CGGGAACTAG CCTAAGCGAG CCGGAGAGGG CTGAACCCGC TGGCCTGGCC 
CGGCGCGGGT ACGCGCCCGC TCCGCGCCGC CTCACCCGTG CGTTTCGGAC TCCGGAGCCG 
TCCTTCCCCT CTCCGTTGAG CCGGTCCGCC GGCGAGAGCC AGAGCTTCCC CCGGCGCCGC 
AGGCCCGGGC CCGCTGAGCC AGGCCTCGTG GGTGCACGCG GGGGCCCGTC ACGTGCGGCG 
TGGATCGCTC GCCCAGCCGG GCACAGCAGG GAAGGCTTGC CCACGAGTGT GCGCGGAGTC 
GGGCGATTGT ATTCACATCG ATCCTCCGAA ATGCTTCGAT TACCCGCAGT CCTTCGTCAA 
ATGAGGCCAG TGTCCAGGGC ACTGGCTCCT CATCTCACTC GGGCTTATGC CAAAGATGTA 
AAATTCGGTG CAGATGCTCG AGCCTTAATG CTTCAAGGTG TAGACCTTTT AGCCGATGCT 
GTAGCCGTTA CTATGGGGCC AAAGGGAAGG ACAGTGATTA TTGAACAGAG TTGGGGAAGT 
CCCAAAGTGA CAAAAGATGG TGTGACTGTT GCAAAGTCTA TTGATTTAAA AGATAAATAT 
AAAAATATTG GCGCTAAACT TGTTCAAGAT GTTGCCAATA ACACAAATGA AGAGGCGGGG 
GATGGCACCA CTACTGCTAC TGTACTGGCA CGCTCTATTG CCAAGGAAGG CTTCGAGAAG 
ATTAGCAAAG GTGCTAATCC AGTGGAAATC AGGAGAGGTG TGATGTTAGC TGTTGATGCT 
GTAATTGCTG AACTTAAGAA GCAGTCTAAA CCTGTGACAA CCCCCGAAGA GATTGCTCAG 
GTTGCTACAA TTTCTGCAAA TGGAGACAAA GAAATTGGCA ACATCATTTC TGATGCCATG 
AAAAAAGTTG GAAGAAAGGG TGTTATCACA GTAAAGGATG GAAAAACACT GAATGATGAA 
TTAGAAATTA TTGAAGGCAT GAAGTTTGAC AGAGGATATA TTTCTCCATA CTTTATTAAT 
ACATCAAAAG GTCAGAAATG TGAATTCCAA GATGCATATG TTCTATTGAG TGAAAAGAAA 
ATTTCTAGTG TCCAATCCAT TGTTCCTGCT CTTGAAATTG CCAATGCTCA CCGGAAGCCC 
TTGGTCATAA TTGCTGAAGA TGTGGATGGA GAAGCTCTAA GTACCCTTGT TTTGAATAGG 
TTGAAGGTTG GTCTTCAAGT TGTGGCAGTC AAAGCTCCAG GTTTTGGTGA CAATAGAAAG 
AACCAGCTTA AAGACATGGC TATTGCTACT GGGGGTGCAG TATTTGGAGA AGAAGGACTA 
AATCTAAATC TTGAAGATGT TCAGCCTCAT GACTTAGGAA AAGTTGGAGA GGTCATTGTG 
ACCAAAGATG ATGCCATGCT CTTGAAAGGA AAAGGTGACA AGGCTCAAAT TGAGAAGCGT 
ATCCAAGAGA TCATTGAGCA GTTAGATATC ACAACTAGTG AATATGAAAA GGAAAAACTG 
AATGAACGTC TGGCAAAACT CTCAGATGGT GTTGCTGTGT TGAAGGTTGG TGGGACAAGT 
GATGTTGAAG TGAATGAAAA GAAAGACAGA GTTACAGATG CTCTTAATGC CACACGAGCT 
GCTGTTGAAG AAGGCATTGT TCTGGGAGGG GGCTGTGCCC TGCTTCGGTG CATTCCAGCC 
TTGGAGTCAA TAACTCCAGC TAATGAAGAT CAAAAAACAG GTATAGAAAT AATTAAAAAA 
ACACTCAAAA TTCCTGCAAT GACCATTGCT AAGAATGCAG GTGTTGAAGG ATCATTGATA 
GTTGAGAAAA TTATGCAGAG TTCTTCAGAA GTCGGTTATG ATGCTATGCT TGGAGATTTT 
GTGAATATGG TGGAAAAAGG AATCATTGAT CCAACTAAGG TTGTAAGAAC TGCATTACTG 
GATGCTGCCG GAGTGGCCTC TCTCTTAACA ACAGCAGAAG TTGTCGTCAC AGAAATTCCT 
AAAGAAGAGA AGGATCCTGG AATGGGTGGC ATGGGTGGAA TGGGAGGTGG CATGGGAGGT 
GGCATGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of Heat Shock Protein 60 as a Regulator of Neutral Sphingomyelinase 2 and Its Role in Dopamine Uptake.
PloS one8(6)e67216(2013)
Ahn KH,Kim SK,Choi JM,Jung SY,Won JH,Back MJ,Fu Z,Jang JM,Ha HC,Kim DK

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Localization of the chaperone proteins GRP78 and HSP60 on the luminal surface of bovine oviduct epithelial cells and their association with spermatozoa.
Biology of reproduction71(6)1879-89(2004 Dec)
Boilard M,Reyes-Moreno C,Lachance C,Massicotte L,Bailey JL,Sirard MA,Leclerc P

Molecular mimicry in Lyme disease: monoclonal antibody H9724 to B. burgdorferi flagellin specifically detects chaperonin-HSP60.
Biochimica et biophysica acta1181(1)97-100(1993 Mar)
Dai Z,Lackland H,Stein S,Li Q,Radziewicz R,Williams S,Sigal LH

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HSPD1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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