Hspd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hspd1
Entrez Gene ID	63868
Full Name	heat shock protein family D member 1
Synonyms	Hsp60,Hspd1-30p
General protein information	Preferred Names heat shock protein family D member 1 Names 60 kDa heat shock protein, mitochondrial 60 kDa chaperonin CPN60 HSP-60 HSP-65 chaperonin 60 heat shock 60kD protein 1 (chaperonin) heat shock 60kDa protein 1 heat shock protein 1 (chaperonin) heat shock protein 60 (liver) mitochondrial matrix protein P1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q31
Summary	a stress-inducible molecular chaperone which participates in protein folding [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006244932.2	XP_006244994.1	60 kDa heat shock protein, mitochondrial isoform X1
NM_022229.2	NP_071565.2	60 kDa heat shock protein, mitochondrial

Pathways from BioSystems

Homologies

	HSPD1	XP_002799053.1
Danio rerio (zebrafish)	hspd1	NP_851847.1
Homo sapiens (human)	HSPD1	NP_955472.1
Gallus gallus (chicken)	HSPD1	NP_001012934.1
Saccharomyces cerevisiae (baker's yeast)	HSP60	NP_013360.1
Schizosaccharomyces pombe (fission yeast)	mcp60	NP_592894.1
Oryza sativa (rice)	Os10g0462900	NP_001064784.1
Pan troglodytes (chimpanzee)	HSPD1	XP_001169056.1
Mus musculus (house mouse)	Hspd1	NP_034607.3
Kluyveromyces lactis	KLLA0F09449g	XP_455510.1
Magnaporthe oryzae (rice blast fungus)	MGG_03165	XP_003716826.1
Arabidopsis thaliana (thale cress)	HSP60	NP_189041.1
Oryza sativa (rice)	Os03g0143400	NP_001048938.1
Canis lupus familiaris (dog)	HSPD1	XP_005640555.1
Bos taurus (cattle)	HSPD1	NP_001160082.1
Drosophila melanogaster (fruit fly)	Hsp60	NP_511115.2
Caenorhabditis elegans (roundworm)	hsp-60	NP_497429.1
Rattus norvegicus (Norway rat)	Hspd1	NP_071565.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004002	XP_318461.2
Eremothecium gossypii	AGOS_AFR155W	NP_985702.2
Neurospora crassa	NCU01589	XP_956500.2
Xenopus tropicalis (tropical clawed frog)	hspd1	NP_001096355.1

Gene Ontology
Bibliography

Related articles in PubMed

Lenti-siRNA Hsp60 promote bax in mitochondria and induces apoptosis during heat stress.
Song E, Tang S, Xu J, Yin B, Bao E, Hartung J
Biochemical and biophysical research communications481(1-2)125-131(2016 Dec)

The pathological role of advanced glycation end products-downregulated heat shock protein 60 in islet ?-cell hypertrophy and dysfunction.
Guan SS, Sheu ML, Yang RS, Chan DC, Wu CT, Yang TH, Chiang CK, Liu SH
Oncotarget7(17)23072-87(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

However, bax in the cytoplasm translocated to mitochondrial during heat stress and regulated the permeability of outer mitochondrial membrane. We hypothesize that Hsp60 can protect cardiac myocytes against apoptosis involving in outer mitochondrial membrane not the inner mitochondrial membrane under heat stress.
Title: Lenti-siRNA Hsp60 promote bax in mitochondria and induces apoptosis during heat stress.

Heat shock protein 60 stimulates the migration of vascular smooth muscle cells via Toll-like receptor 4 and ERK MAPK activation
Title: Heat shock protein 60 stimulates the migration of vascular smooth muscle cells via Toll-like receptor 4 and ERK MAPK activation.

Cytosolic Hsp60 orchestrates the survival and inflammatory responses of vascular smooth muscle cells in injured aortic vessels.
Title: Cytosolic Hsp60 orchestrates the survival and inflammatory responses of vascular smooth muscle cells in injured aortic vessels.

Hsp60 is increased in both animals and patients with TLE in affected tissues, and in plasma in response to epileptic seizures, and point to it as biomarker of hippocampal stress potentially useful for diagnosis and patient management.
Title: Hsp60 response in experimental and human temporal lobe epilepsy.

Hsp60 represents a potential biomarker of heat stress, and may protect against heat stress induced myocardial cellular damage both in vivo and in vitro.
Title: Association of Hsp60 expression with damage to rat myocardial cells exposed to heat stress in vivo and in vitro.

The following Hspd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hspd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12783	NM_022229.2 Latest version!	Rattus norvegicus heat shock protein family D (Hsp60) member 1 (Hspd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa12783	XM_006244932.2 Latest version!	Rattus norvegicus heat shock protein family D member 1 (Hspd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hspd1 ( NM_022229.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071565.2 Rattus norvegicus heat shock protein family D (Hsp60) member 1 (Hspd1), mRNA.

Price & Availability↑

CloneID	ORa12783
Clone ID Related Accession (Same CDS sequence)	XM_006244932.2 , NM_022229.2
Accession Version	NM_022229.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	60 kDa heat shock protein, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC086507.1. On Sep 25, 2008 this sequence version replaced NM_022229.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC086507.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00632254, SAMN01906348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCTTCGAC TACCCACAGT CCTTCGCCAG ATGAGACCAG TGTCTCGGGC ACTGGCTCCT 
CATCTCACTC GGGCCTATGC CAAAGATGTA AAATTTGGTG CGGATGCTCG AGCCTTAATG 
CTTCAAGGTG TAGACCTTTT AGCCGATGCT GTAGCTGTTA CAATGGGGCC AAAGGGAAGA 
ACAGTGATTA TTGAACAGAG TTGGGGAAGT CCCAAAGTAA CAAAAGATGG GGTCACTGTT 
GCAAAGTCAA TTGATTTAAA GGATAAATAC AAAAATATCG GAGCTAAGCT TGTTCAGGAT 
GTTGCCAATA ACACAAATGA AGAGGCTGGG GATGGCACCA CCACTGCCAC TGTTCTGGCA 
CGGTCTATTG CCAAGGAGGG CTTTGAGAAG ATCAGCAAAG GGGCTAATCC AGTGGAAATC 
CGGAGAGGTG TGATGTTGGC TGTTGATGCT GTAATTGCTG AACTTAAGAA ACAATCTAAA 
CCTGTGACAA CCCCTGAAGA AATTGCTCAG GTTGCTACAA TTTCTGCAAA CGGAGACAAA 
GACATTGGGA ACATCATTTC TGATGCAATG AAGAAGGTTG GAAGAAAGGG TGTCATCACA 
GTGAAGGATG GAAAAACCCT GAATGATGAG CTAGAAATTA TTGAAGGCAT GAAGTTTGAT 
AGAGGATATA TTTCCCCATA TTTTATTAAC ACATCAAAAG GTCAAAAATG TGAATTCCAA 
GATGCCTATG TTTTGTTGAG TGAAAAGAAA ATTTCTAGTG TTCAGTCCAT TGTACCTGCT 
CTTGAAATTG CCAATGCTCA CCGGAAGCCC TTGGTCATAA TTGCTGAAGA TGTTGATGGA 
GAAGCTCTTA GCACACTGGT TTTGAACAGG CTAAAAGTTG GTCTTCAGGT TGTAGCAGTC 
AAAGCTCCAG GGTTTGGGGA CAACAGGAAG AACCAGCTTA AAGATATGGC TATCGCTACT 
GGTGGTGCGG TGTTTGGAGA AGAGGGTTTG AATCTAAATC TTGAAGATGT TCAAGCTCAT 
GATTTAGGGA AAGTTGGAGA GGTCATCGTC ACCAAAGATG ATGCCATGCT TTTGAAAGGA 
AAAGGTGACA AAGCTCACAT TGAAAAACGT ATTCAAGAAA TCACTGAGCA GCTAGACATC 
ACAACTAGTG AATATGAGAA GGAAAAGCTG AACGAGCGAC TTGCTAAACT CTCAGATGGA 
GTAGCTGTGT TGAAGGTTGG AGGGACAAGT GATGTTGAAG TGAATGAGAA GAAAGACAGA 
GTTACAGATG CTCTCAATGC TACAAGAGCA GCTGTTGAAG AAGGCATTGT TCTAGGAGGG 
GGCTGTGCTC TACTTCGGTG CATCCCAGCC TTGGATTCAT TAAAGCCTGC TAATGAAGAT 
CAGAAGATAG GTATAGAAAT TATTAAAAGA GCACTTAAAA TTCCTGCAAT GACAATTGCT 
AAGAATGCAG GTGTTGAAGG ATCTTTGATA GTTGAAAAAA TTCTGCAGAG TTCCTCAGAG 
GTTGGCTATG ATGCCATGCT TGGAGATTTT GTGAACATGG TGGAAAAGGG AATCATTGAT 
CCAACAAAGG TTGTAAGAAC TGCTTTACTG GATGCTGCTG GGGTGGCCTC CCTGCTAACT 
ACAGCCGAAG CTGTAGTGAC AGAAATTCCT AAAGAAGAGA AGGACCCTGG AATGGGTGCA 
ATGGGTGGAA TGGGAGGGGG TATGGGAGGT GGCATGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071565.2
CDS	59..1780
Translation	MLRLPTVLRQMRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKDIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSVQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLNLNLEDVQAHDLGKVGEVIVTKDDAMLLKGKGDKAHIEKRIQEITEQLDITTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLKPANEDQKIGIEIIKRALKIPAMTIAKNAGVEGSLIVEKILQSSSEVGYDAMLGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEAVVTEIPKEEKDPGMGAMGGMGGGMGGGMF

Target ORF information:

RefSeq Version	NM_022229.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus heat shock protein family D (Hsp60) member 1 (Hspd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022229.2

ORF Insert Sequence:

ATGCTTCGAC TACCCACAGT CCTTCGCCAG ATGAGACCAG TGTCTCGGGC ACTGGCTCCT 
CATCTCACTC GGGCCTATGC CAAAGATGTA AAATTTGGTG CGGATGCTCG AGCCTTAATG 
CTTCAAGGTG TAGACCTTTT AGCCGATGCT GTAGCTGTTA CAATGGGGCC AAAGGGAAGA 
ACAGTGATTA TTGAACAGAG TTGGGGAAGT CCCAAAGTAA CAAAAGATGG GGTCACTGTT 
GCAAAGTCAA TTGATTTAAA GGATAAATAC AAAAATATCG GAGCTAAGCT TGTTCAGGAT 
GTTGCCAATA ACACAAATGA AGAGGCTGGG GATGGCACCA CCACTGCCAC TGTTCTGGCA 
CGGTCTATTG CCAAGGAGGG CTTTGAGAAG ATCAGCAAAG GGGCTAATCC AGTGGAAATC 
CGGAGAGGTG TGATGTTGGC TGTTGATGCT GTAATTGCTG AACTTAAGAA ACAATCTAAA 
CCTGTGACAA CCCCTGAAGA AATTGCTCAG GTTGCTACAA TTTCTGCAAA CGGAGACAAA 
GACATTGGGA ACATCATTTC TGATGCAATG AAGAAGGTTG GAAGAAAGGG TGTCATCACA 
GTGAAGGATG GAAAAACCCT GAATGATGAG CTAGAAATTA TTGAAGGCAT GAAGTTTGAT 
AGAGGATATA TTTCCCCATA TTTTATTAAC ACATCAAAAG GTCAAAAATG TGAATTCCAA 
GATGCCTATG TTTTGTTGAG TGAAAAGAAA ATTTCTAGTG TTCAGTCCAT TGTACCTGCT 
CTTGAAATTG CCAATGCTCA CCGGAAGCCC TTGGTCATAA TTGCTGAAGA TGTTGATGGA 
GAAGCTCTTA GCACACTGGT TTTGAACAGG CTAAAAGTTG GTCTTCAGGT TGTAGCAGTC 
AAAGCTCCAG GGTTTGGGGA CAACAGGAAG AACCAGCTTA AAGATATGGC TATCGCTACT 
GGTGGTGCGG TGTTTGGAGA AGAGGGTTTG AATCTAAATC TTGAAGATGT TCAAGCTCAT 
GATTTAGGGA AAGTTGGAGA GGTCATCGTC ACCAAAGATG ATGCCATGCT TTTGAAAGGA 
AAAGGTGACA AAGCTCACAT TGAAAAACGT ATTCAAGAAA TCACTGAGCA GCTAGACATC 
ACAACTAGTG AATATGAGAA GGAAAAGCTG AACGAGCGAC TTGCTAAACT CTCAGATGGA 
GTAGCTGTGT TGAAGGTTGG AGGGACAAGT GATGTTGAAG TGAATGAGAA GAAAGACAGA 
GTTACAGATG CTCTCAATGC TACAAGAGCA GCTGTTGAAG AAGGCATTGT TCTAGGAGGG 
GGCTGTGCTC TACTTCGGTG CATCCCAGCC TTGGATTCAT TAAAGCCTGC TAATGAAGAT 
CAGAAGATAG GTATAGAAAT TATTAAAAGA GCACTTAAAA TTCCTGCAAT GACAATTGCT 
AAGAATGCAG GTGTTGAAGG ATCTTTGATA GTTGAAAAAA TTCTGCAGAG TTCCTCAGAG 
GTTGGCTATG ATGCCATGCT TGGAGATTTT GTGAACATGG TGGAAAAGGG AATCATTGAT 
CCAACAAAGG TTGTAAGAAC TGCTTTACTG GATGCTGCTG GGGTGGCCTC CCTGCTAACT 
ACAGCCGAAG CTGTAGTGAC AGAAATTCCT AAAGAAGAGA AGGACCCTGG AATGGGTGCA 
ATGGGTGGAA TGGGAGGGGG TATGGGAGGT GGCATGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hspd1 ( XM_006244932.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006244994.1 Rattus norvegicus heat shock protein family D member 1 (Hspd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa12783
Clone ID Related Accession (Same CDS sequence)	XM_006244932.2 , NM_022229.2
Accession Version	XM_006244932.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	60 kDa heat shock protein, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006244932.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAC TACCCACAGT CCTTCGCCAG ATGAGACCAG TGTCTCGGGC ACTGGCTCCT 
CATCTCACTC GGGCCTATGC CAAAGATGTA AAATTTGGTG CGGATGCTCG AGCCTTAATG 
CTTCAAGGTG TAGACCTTTT AGCCGATGCT GTAGCTGTTA CAATGGGGCC AAAGGGAAGA 
ACAGTGATTA TTGAACAGAG TTGGGGAAGT CCCAAAGTAA CAAAAGATGG GGTCACTGTT 
GCAAAGTCAA TTGATTTAAA GGATAAATAC AAAAATATCG GAGCTAAGCT TGTTCAGGAT 
GTTGCCAATA ACACAAATGA AGAGGCTGGG GATGGCACCA CCACTGCCAC TGTTCTGGCA 
CGGTCTATTG CCAAGGAGGG CTTTGAGAAG ATCAGCAAAG GGGCTAATCC AGTGGAAATC 
CGGAGAGGTG TGATGTTGGC TGTTGATGCT GTAATTGCTG AACTTAAGAA ACAATCTAAA 
CCTGTGACAA CCCCTGAAGA AATTGCTCAG GTTGCTACAA TTTCTGCAAA CGGAGACAAA 
GACATTGGGA ACATCATTTC TGATGCAATG AAGAAGGTTG GAAGAAAGGG TGTCATCACA 
GTGAAGGATG GAAAAACCCT GAATGATGAG CTAGAAATTA TTGAAGGCAT GAAGTTTGAT 
AGAGGATATA TTTCCCCATA TTTTATTAAC ACATCAAAAG GTCAAAAATG TGAATTCCAA 
GATGCCTATG TTTTGTTGAG TGAAAAGAAA ATTTCTAGTG TTCAGTCCAT TGTACCTGCT 
CTTGAAATTG CCAATGCTCA CCGGAAGCCC TTGGTCATAA TTGCTGAAGA TGTTGATGGA 
GAAGCTCTTA GCACACTGGT TTTGAACAGG CTAAAAGTTG GTCTTCAGGT TGTAGCAGTC 
AAAGCTCCAG GGTTTGGGGA CAACAGGAAG AACCAGCTTA AAGATATGGC TATCGCTACT 
GGTGGTGCGG TGTTTGGAGA AGAGGGTTTG AATCTAAATC TTGAAGATGT TCAAGCTCAT 
GATTTAGGGA AAGTTGGAGA GGTCATCGTC ACCAAAGATG ATGCCATGCT TTTGAAAGGA 
AAAGGTGACA AAGCTCACAT TGAAAAACGT ATTCAAGAAA TCACTGAGCA GCTAGACATC 
ACAACTAGTG AATATGAGAA GGAAAAGCTG AACGAGCGAC TTGCTAAACT CTCAGATGGA 
GTAGCTGTGT TGAAGGTTGG AGGGACAAGT GATGTTGAAG TGAATGAGAA GAAAGACAGA 
GTTACAGATG CTCTCAATGC TACAAGAGCA GCTGTTGAAG AAGGCATTGT TCTAGGAGGG 
GGCTGTGCTC TACTTCGGTG CATCCCAGCC TTGGATTCAT TAAAGCCTGC TAATGAAGAT 
CAGAAGATAG GTATAGAAAT TATTAAAAGA GCACTTAAAA TTCCTGCAAT GACAATTGCT 
AAGAATGCAG GTGTTGAAGG ATCTTTGATA GTTGAAAAAA TTCTGCAGAG TTCCTCAGAG 
GTTGGCTATG ATGCCATGCT TGGAGATTTT GTGAACATGG TGGAAAAGGG AATCATTGAT 
CCAACAAAGG TTGTAAGAAC TGCTTTACTG GATGCTGCTG GGGTGGCCTC CCTGCTAACT 
ACAGCCGAAG CTGTAGTGAC AGAAATTCCT AAAGAAGAGA AGGACCCTGG AATGGGTGCA 
ATGGGTGGAA TGGGAGGGGG TATGGGAGGT GGCATGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006244994.1
CDS	95..1816
Translation	MLRLPTVLRQMRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKDIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSVQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLNLNLEDVQAHDLGKVGEVIVTKDDAMLLKGKGDKAHIEKRIQEITEQLDITTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLKPANEDQKIGIEIIKRALKIPAMTIAKNAGVEGSLIVEKILQSSSEVGYDAMLGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEAVVTEIPKEEKDPGMGAMGGMGGGMGGGMF

Target ORF information:

RefSeq Version	XM_006244932.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus heat shock protein family D member 1 (Hspd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006244932.2

ORF Insert Sequence:

ATGCTTCGAC TACCCACAGT CCTTCGCCAG ATGAGACCAG TGTCTCGGGC ACTGGCTCCT 
CATCTCACTC GGGCCTATGC CAAAGATGTA AAATTTGGTG CGGATGCTCG AGCCTTAATG 
CTTCAAGGTG TAGACCTTTT AGCCGATGCT GTAGCTGTTA CAATGGGGCC AAAGGGAAGA 
ACAGTGATTA TTGAACAGAG TTGGGGAAGT CCCAAAGTAA CAAAAGATGG GGTCACTGTT 
GCAAAGTCAA TTGATTTAAA GGATAAATAC AAAAATATCG GAGCTAAGCT TGTTCAGGAT 
GTTGCCAATA ACACAAATGA AGAGGCTGGG GATGGCACCA CCACTGCCAC TGTTCTGGCA 
CGGTCTATTG CCAAGGAGGG CTTTGAGAAG ATCAGCAAAG GGGCTAATCC AGTGGAAATC 
CGGAGAGGTG TGATGTTGGC TGTTGATGCT GTAATTGCTG AACTTAAGAA ACAATCTAAA 
CCTGTGACAA CCCCTGAAGA AATTGCTCAG GTTGCTACAA TTTCTGCAAA CGGAGACAAA 
GACATTGGGA ACATCATTTC TGATGCAATG AAGAAGGTTG GAAGAAAGGG TGTCATCACA 
GTGAAGGATG GAAAAACCCT GAATGATGAG CTAGAAATTA TTGAAGGCAT GAAGTTTGAT 
AGAGGATATA TTTCCCCATA TTTTATTAAC ACATCAAAAG GTCAAAAATG TGAATTCCAA 
GATGCCTATG TTTTGTTGAG TGAAAAGAAA ATTTCTAGTG TTCAGTCCAT TGTACCTGCT 
CTTGAAATTG CCAATGCTCA CCGGAAGCCC TTGGTCATAA TTGCTGAAGA TGTTGATGGA 
GAAGCTCTTA GCACACTGGT TTTGAACAGG CTAAAAGTTG GTCTTCAGGT TGTAGCAGTC 
AAAGCTCCAG GGTTTGGGGA CAACAGGAAG AACCAGCTTA AAGATATGGC TATCGCTACT 
GGTGGTGCGG TGTTTGGAGA AGAGGGTTTG AATCTAAATC TTGAAGATGT TCAAGCTCAT 
GATTTAGGGA AAGTTGGAGA GGTCATCGTC ACCAAAGATG ATGCCATGCT TTTGAAAGGA 
AAAGGTGACA AAGCTCACAT TGAAAAACGT ATTCAAGAAA TCACTGAGCA GCTAGACATC 
ACAACTAGTG AATATGAGAA GGAAAAGCTG AACGAGCGAC TTGCTAAACT CTCAGATGGA 
GTAGCTGTGT TGAAGGTTGG AGGGACAAGT GATGTTGAAG TGAATGAGAA GAAAGACAGA 
GTTACAGATG CTCTCAATGC TACAAGAGCA GCTGTTGAAG AAGGCATTGT TCTAGGAGGG 
GGCTGTGCTC TACTTCGGTG CATCCCAGCC TTGGATTCAT TAAAGCCTGC TAATGAAGAT 
CAGAAGATAG GTATAGAAAT TATTAAAAGA GCACTTAAAA TTCCTGCAAT GACAATTGCT 
AAGAATGCAG GTGTTGAAGG ATCTTTGATA GTTGAAAAAA TTCTGCAGAG TTCCTCAGAG 
GTTGGCTATG ATGCCATGCT TGGAGATTTT GTGAACATGG TGGAAAAGGG AATCATTGAT 
CCAACAAAGG TTGTAAGAAC TGCTTTACTG GATGCTGCTG GGGTGGCCTC CCTGCTAACT 
ACAGCCGAAG CTGTAGTGAC AGAAATTCCT AAAGAAGAGA AGGACCCTGG AATGGGTGCA 
ATGGGTGGAA TGGGAGGGGG TATGGGAGGT GGCATGTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lenti-siRNA Hsp60 promote bax in mitochondria and induces apoptosis during heat stress.
Biochemical and biophysical research communications481(1-2)125-131(2016 Dec)
Song E,Tang S,Xu J,Yin B,Bao E,Hartung J

The pathological role of advanced glycation end products-downregulated heat shock protein 60 in islet ?-cell hypertrophy and dysfunction.
Oncotarget7(17)23072-87(2016 Apr)
Guan SS,Sheu ML,Yang RS,Chan DC,Wu CT,Yang TH,Chiang CK,Liu SH

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Hspd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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