ptprfb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ptprfb
Entrez Gene ID	799867
Full Name	protein tyrosine phosphatase, receptor type, f, b
Synonyms	lar,ptprf,si:dkey-21k10.1,wu:fj23e12
General protein information	Preferred Names protein tyrosine phosphatase, receptor type, f, b Names receptor-type tyrosine-protein phosphatase F fj23e12
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009298459.3	XP_009296734.1	receptor-type tyrosine-protein phosphatase F isoform X2
XM_009298461.3	XP_009296736.1	receptor-type tyrosine-protein phosphatase F isoform X4
XM_021481419.1	XP_021337094.1	receptor-type tyrosine-protein phosphatase F isoform X7
XM_009298464.3	XP_009296739.1	receptor-type tyrosine-protein phosphatase F isoform X8
XM_009298466.3	XP_009296741.1	receptor-type tyrosine-protein phosphatase F isoform X9
XM_009298467.3	XP_009296742.1	receptor-type tyrosine-protein phosphatase F isoform X10
XM_021481437.1	XP_021337112.1	receptor-type tyrosine-protein phosphatase F isoform X12
XM_021481440.1	XP_021337115.1	receptor-type tyrosine-protein phosphatase F isoform X15
XM_009298469.2	XP_009296744.1	receptor-type tyrosine-protein phosphatase F isoform X11
XM_009298458.2	XP_009296733.1	receptor-type tyrosine-protein phosphatase F isoform X1
XM_009298462.2	XP_009296737.1	receptor-type tyrosine-protein phosphatase F isoform X5
XM_009298457.3	XP_009296732.1	receptor-type tyrosine-protein phosphatase F isoform X1
XM_009298463.3	XP_009296738.1	receptor-type tyrosine-protein phosphatase F isoform X6
XM_021481438.1	XP_021337113.1	receptor-type tyrosine-protein phosphatase F isoform X13
XM_009298459.2	XP_009296734.1	receptor-type tyrosine-protein phosphatase F isoform X2
XM_009298460.2	XP_009296735.1	receptor-type tyrosine-protein phosphatase F isoform X3
XM_009298463.2	XP_009296738.1	receptor-type tyrosine-protein phosphatase F isoform X6
XM_009298464.2	XP_009296739.1	receptor-type tyrosine-protein phosphatase F isoform X7
XM_009298468.2	XP_009296743.1	receptor-type tyrosine-protein phosphatase F isoform X10
XM_021481410.1	XP_021337085.1	receptor-type tyrosine-protein phosphatase F isoform X1
NM_001083576.1	NP_001077045.1	receptor-type tyrosine-protein phosphatase F precursor
XM_009298467.2	XP_009296742.1	receptor-type tyrosine-protein phosphatase F isoform X9
XM_009298470.2	XP_009296745.1	receptor-type tyrosine-protein phosphatase F isoform X12
XM_009298460.3	XP_009296735.1	receptor-type tyrosine-protein phosphatase F isoform X3
XM_009298462.3	XP_009296737.1	receptor-type tyrosine-protein phosphatase F isoform X5
XM_021481443.1	XP_021337118.1	receptor-type tyrosine-protein phosphatase F isoform X17
XM_009298457.2	XP_009296732.1	receptor-type tyrosine-protein phosphatase F isoform X1
XM_009298461.2	XP_009296736.1	receptor-type tyrosine-protein phosphatase F isoform X4
XM_009298466.2	XP_009296741.1	receptor-type tyrosine-protein phosphatase F isoform X8
XM_009298458.3	XP_009296733.1	receptor-type tyrosine-protein phosphatase F isoform X1
XM_009298468.3	XP_009296743.1	receptor-type tyrosine-protein phosphatase F isoform X11
XM_009298469.3	XP_009296744.1	receptor-type tyrosine-protein phosphatase F isoform X14
XM_021481441.1	XP_021337116.1	receptor-type tyrosine-protein phosphatase F isoform X16

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ptprf	NP_062122.1
Danio rerio (zebrafish)	ptprfb	NP_001077045.1
Danio rerio (zebrafish)	ptprfa	XP_692206.6
	PTPRF	XP_001095521.2
Pan troglodytes (chimpanzee)	PTPRF	XP_001173898.2
Canis lupus familiaris (dog)	PTPRF	XP_005629167.1
Gallus gallus (chicken)	PTPRF	XP_004936750.1
Homo sapiens (human)	PTPRF	NP_002831.2
Mus musculus (house mouse)	Ptprf	NP_035343.2
Bos taurus (cattle)	PTPRF	NP_001094549.1
Xenopus tropicalis (tropical clawed frog)	ptprf	XP_004914019.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolutionary recruitment of flexible Esrp-dependent splicing programs into diverse embryonic morphogenetic processes.
Burguera D, Marquez Y, Racioppi C, Permanyer J, Torres-M?ndez A, Esposito R, Albuixech-Crespo B, Fanlo L, D'Agostino Y, Gohr A, Navas-Perez E, Riesgo A, Cuomo C, Benvenuto G, Christiaen LA, Mart? E, D'Aniello S, Spagnuolo A, Ristoratore F, Arnone MI, Garcia-Fern?ndez J, Irimia M
Nature communications8(1)1799(2017 Nov)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Integrative view of ?2,3-sialyltransferases (ST3Gal) molecular and functional evolution in deuterostomes: significance of lineage-specific losses.
Petit D, Teppa E, Mir AM, Vicogne D, Thisse C, Thisse B, Filloux C, Harduin-Lepers A
Molecular biology and evolution32(4)906-27(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ptprfb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ptprfb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa150375	XM_021481419.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150376	XM_021481437.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150377	XM_021481438.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150378	XM_021481441.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150379	XM_021481443.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa00569	NM_001083576.1 Latest version!	Danio rerio protein tyrosine phosphatase receptor type Fb (ptprfb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47413	XM_009298458.3 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47413	XM_009298457.3 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47413	XM_021481410.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47414	XM_009298459.3 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47415	XM_009298460.3 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47416	XM_009298461.3 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47417	XM_009298462.3 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47418	XM_009298463.3 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47419	XM_009298464.3 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47420	XM_009298466.3 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47421	XM_009298467.3 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47422	XM_009298468.3 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47423	XM_009298469.3 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47424	XM_021481440.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47424	XM_009298470.2 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa47414	XM_009298459.2	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47418	XM_009298463.2	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47419	XM_009298464.2	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47421	XM_009298467.2	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47415	XM_009298460.2	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47417	XM_009298462.2	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47413	XM_009298457.2	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47416	XM_009298461.2	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47420	XM_009298466.2	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47413	XM_009298458.2	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47422	XM_009298468.2	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa47423	XM_009298469.2	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ptprfb ( XM_021481419.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337094.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa150375
Clone ID Related Accession (Same CDS sequence)	XM_021481419.1
Accession Version	XM_021481419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGACCCTCGA GGAGTACGAC GAGTTCCACC CCGTTTCTCC 
ATCCCACCTA CCAATCACGA GGTGATGCCA GGGGGCAGTG TAAACCTCAC CTGCGTGGCA 
GTAGGTGCCC CGATGCCCTA CGTCAAATGG ATGACTGGTG AGGTGGAGCT CACCAAGGAT 
GAGGAGATGC CTGTGGGACG CAACGTCTTG GAGCTCACCA ACATCCGTCA GTCGACCAAC 
TACACCTGCG TGGCTATATC ATCCCTAGGT ATGATCGAGG CAACGGCACA AGTGTCTGTC 
AAAGCTCTCC CAAAGCCTCC TACATCCCTG ACTGTTACGG AAACCACAGC TACGAGTGTT 
ACACTCACAT GGGACTCTGG AAACCCTGAA CCTGTGTCTT ACTACATAAT TCAATACCGG 
GCCAAGACTT CAGACACCAA CTTCCAAGAA GTGGACGGTG TGGCCACCAC ACGTTACAGC 
ATCGGCGGTC TCAGCCCATA TTCAGAGTAT GAGTTTCGAG TAATGGCAGT CAACAACATT 
GGTCGTGGGC CACCTAGTGA ACCCGTTGAA ACACGAACAG GTGAACAGGC ACCTTCCTCC 
CCACCGCTGC ATGTTCAAGC ACGAATGCTG AGTGCCAGCA CCATGTTGGT TCAGTGGGAA 
CCACCAGAGG AACCCAATGG TCAGATCAGA GGCTATCGGA TCTACTACAC ATCTGACCTG 
GATGCACCTC TTAGCGCCTG GCAGAAACAC AACACAGACG ATAGCCGTCT CACCACTATC 
TCCAGCCTGA CCACCGGCAT CACCTACAGC CTACGTGTGC TGGGCTTCAC ATCTGTAGGG 
GATGGACCAC CATCTGATGT GCTACAGGTC AAGACACAGC AAGGAGTTCC TGCTCAGCCA 
TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT ACAAGGATCC TACTAACTTG GCTGTGGCCC 
GTGCAGGACC CCATCATCAA ATACGAGCTG CAATATTGGG AGGCTGACTC AGACAACAAG 
ATCCATGTCA CCTTTGACCC AGCTGGCTCG TACGCTGTTG AAGGTCTGAA ACCAGACACT 
TTGTACAAAT TCTCTCTGGC AGCCCGCTCT GAAATGGGAC AAGGAGTCTT CACTCACCCC 
ATTGAAGCCA GGACGGCCCA GTCTGCCCCC TCTGCCCCCC CTCAAGACGT GCACCTGCTC 
AGTCTGAGCT CCACCAGCAT CAAGGTGAGT TGGGTGGCAC CACCCGCCGC TAGCCGCCAC 
GGCGCAATAG TGCGCTACAC AGTCAGCTAC CAGGCAGTGA ATGGAGAAGA CACAGAGCGC 
CATGAGGTGC CGGACATTGG AGCGGATGCC TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG 
TGGACGGAGT ATCAGGTGTG GGTGAGAGCG CACACTGATG TGGGCCCCGG CCCCGAGAGC 
ACGGCTGTGC GTATCCGAAC CAATGAAGAC GTGCCAGGAG CACCGCCTCG GAAATTGGAA 
GTGGAGGCTT TGAACTCCAC CGCTATCCGT GTGACATGGA AACCTCCTCT TTCTGGAAAG 
CAGCATGGCC AGATCCGGGG ATATCAGGTC ATATACTCCC GGTTGGAGAA CGGGGAACCA 
CGGGGACATC CGAATATAAT GGATGTCGCT CTGCCAGAAG CACAGTGGAA AATTGAAGAG 
TCTACGGAAT ATGAGGCTGT GATCGCTGGA CTTGCCTCTG AAACCTCTTA CTCAGTGACT 
GTGGCTGCGT ACACAACCAA AGGTGATGGA GCTCGCAGCA AAGCCAAAGT GGTCACCACC 
ACCGGAGCAG TGCCTGGCAA ACCCACTATG ATCATCAGCA CCACCGTTGG CAACACGGCC 
CTTATCCAGT GGCAGCCTCC TAAAGACATG GTGGGTGAGC TGATGGGCTA CCGGCTGCGC 
TACAAACGTC TGGAAGAGGA AAACTATGAG ATACGCGAAT TCAGTCGCAC CGACGACCAC 
CACACCGTCA CTGACCTGCA CAAGGGCGCC ACCTACAGCT TCCACCTGAG CGCCAGAAAC 
CGCGCTGGAC TCGGAGAGGA GTACGTTAAA GACATCGGCA CTCCTGAGGA CGTGCCCAGC 
GGGTTTCCTC TTAACCTGCA GGTCGTAGGC TTGACTACCT CTACCACCCG ACTGACCTGG 
GAGCCTCCAG CGTTGTCTGA ACGTAACGGA CGGATCATAC ATTACATTGT GGTTTACCGA 
GACATCAACA GCAAACAAAA CAGCACCAAC CGAACCTCCG ACACACAGAT GACCATCCAA 
GGGCTCCGAC CTGACACCAC CTATGATATC CGTGTGCAGG CGTTCACTAG CAAGGGTGGA 
GGTCCCATAA GTCCCAGTAT CCAGAGCAGG ACCATGTCTA CGTCACCTGC ATTTGCCACA 
AGTTTTGGTG TCAAAGCTGT GATGAAAACC TCTGTCCTTC TCACCTGGGA GGTGCCAGAA 
AATTATAAAT CACAAGTGCC TTTTAAGATC CTCTATAACC AGCAGAGTGT GGAAGTCCAG 
GGGAATCTGA AGAGGAAGCT GATCACACGC CTGCAGCCGG ACACTGATTA CTCGTTTGTG 
CTGATGAGCC GGGGAAACAG CGCCGGTGGC CTACAACAGC AGGTCTCCAT CCGCACTGCA 
CCAGACCTGT TCAAAACTAA ACCTGTCCTG TACACGGCAG ACCAGACCAA CAATGGCAAA 
CTCACCATCA ACCTGCCCAA AGTGCCCACC TCTGCTCCTG TCAGGTGTTA TTACATTGTG 
GTCGTGCCCG TCTCTCAGAC ATCATCTCGT CAGTGGATTA ATCCCGATGA GATGGAGCTG 
GATGAGCTCC TGGAGTCTAG TGAGGGAGCT CATCTCAGGA GACGTCGACG GCACACAGAC 
ACCGTAAGAC CGTACATCGC CGCAAAGCTA TCCACGCTCC CAGAAACCTT CACTCTGGGC 
GATGAGCTAG ACTACAGCGG CTTCATCAAC CGGCCTCTTC CCAACAACCA ACACTTCCAG 
TGTTTTGTGA TGGCTGAACT CAAGGACCAA TACCCTGTTA CTGCCAATGA GAAGCAGCGG 
ACCTTCTCGG CTAGCCCATA CTCAGACCCC ATCATAGTGC AGGGTGAAAA CCAAATGCAG 
CGCAGTGTGG ACCAACCGGA GATGCTTTGG GTCATGGGCC CTGTGCTCGC TGTGGTGCTC 
ATCATCATCA TAGTCATCGC CATCCTGCTC TTCAAAAGCA AACAGGAGAG GAAGAGGGCT 
TCCCCCCTAC CAAAAGACGA TCATTCGGGT GGTGTGAAGG ATTGTCTTCT GGCCAGCTCA 
TCCGATCCTG TCGAAATGAG GAGACTCAAC TACCAGACAC CAGGAATGAG GGAACATCCT 
CCCATATCCG TCTCCGAACT GGCAGACCAC ATCGAGCGCC TCAAGGCCAA CGATGCCCTC 
CGGTTTTCCC AAGAGTATGA GTCTATTGAT CCAGGCCAGC AGTTCACATG GGAGAACTCC 
AACTTGGAGG TCAACAAGCC AAAGAACCGT TATGCCAACG TTATTGCTTA CGACCACTCG 
AGAGTTGTTC TCACCCCTGT GGACGGTGTA CCAGGGAGTG ACTACATTAA TTCCAACTAC 
ATTGATGGCT ACCGTAAGCA GAATGCCTAC ATTGCCACAC AAGGTCCTCT GCCTGAGACT 
CTGAGCGACT TCTGGAGGAT GATTTGGGAG CAACGAGCCA GTACCATCGT CATGATGACC 
AGACTGGAAG AGAAGTCCAG AGTCAAGTGT GACCAATACT GGCCCATTCG TGGCACAGAG 
ACTTATGGAA TGATTCAAGT GACCATGCTT GATGCTGTTG AGCTGGCAAC CTACAGTGTG 
CGCACCTTTG CCCTTTACAA GAATGGCTCT AGTGAGAAGC GCGAGGTCAG GCAGTTTCAG 
TTCATGGCTT GGCCGGACCA CGGAGTGCCT GAATATCCCA CCCCCATCCT AGCCTTCCTG 
CGCAGGGTCA AAGCCTGCAA CCCTCCTGAT GCTGGACCCA TGGTAGTGCA CTGCAGTGCT 
GGAGTTGGCC GTACAGGCTG CTTCATAGTC ATCGATGCGA TGTTGGAGCG CATGAAGCAC 
GAGAAGACCG TAGACATTTA TGGCCACGTC ACGTGCATGA GATCCCAGAG GAACTATATG 
GTGCAGACGG AGGACCAGTA CATCTTTATC CACGAGGCCC TTCTGGAGGC CGCCACCTGT 
GGAAACACAG AGGTCCCTGC CCGAAACCTC TACGCCCACA TCCAGAAGCT CACACAGCCA 
TCGGCTGGAG AGACCGTCAC AGCCATGGAG CTGGAGTTTA AGAGACTCGC CAACTCCAAA 
GCCCACACCT CAAGATTCAT CAGCGCCAAC CTGCCCTGCA ACAAGTTCAA GAACCGGCTG 
GTCAATATCA TGCCTTTCGA GTCCACACGG GTGTGCCTGC AGCCAATCCG AGGAGTGGAG 
GGGTCCGACT ACATCAATGC CAGCTGCATT GACGGATACA GACAGCAGAA GGCCTATATT 
GCCACTCAGG GTCCTCTAGC GGAGACCACC GAGGACTTCT GGAGGATGTT ATGGGAGCAC 
AATTCCACTA TTGTAGTGAT GCTGACCAAA CTCAGAGAGA TGGGACGGGA GAAGTGTCAT 
CAGTACTGGC CCGCCGAGCG CTCAGCCCGC TATCAGTACT TCGTAGTGGA TCCCATGGCC 
GAATACAACA TGCCCCAGTA CATCCTGCGA GAGTTCAAAG TCACAGATGC CAGGGACGGT 
CAATCCAGAA CCATTCGCCA GTTCCAGTTC ACTGATTGGC CAGAGCAGGG AGTGCCAAAA 
ACCGGTGAGG GGTTCATCGA TTTCATTGGG CAAGTTCATA AAACCAAGGA GCAGTTTGGG 
CAGGATGGAC CAATCACTGT GCACTGCAGT GCTGGAGTGG GAAGAACAGG TGTTTTCATC 
ACTCTCAGTA TTGTCCTGGA GAGGATGAGA TATGAGGGGG TGGTGGATCT GTTCCAGACG 
GTTAAAACCC TGCGCACACA AAGGCCTGCC ATGGTGCAGA CAGAGGACCA GTACCAGCTG 
TGCTACAGAG CCGCTCTGGA GTACCTGGGG AGCTTTGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337094.1
CDS	422..6175
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRDPRGVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021481419.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481419.1

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGACCCTCGA GGAGTACGAC GAGTTCCACC CCGTTTCTCC 
ATCCCACCTA CCAATCACGA GGTGATGCCA GGGGGCAGTG TAAACCTCAC CTGCGTGGCA 
GTAGGTGCCC CGATGCCCTA CGTCAAATGG ATGACTGGTG AGGTGGAGCT CACCAAGGAT 
GAGGAGATGC CTGTGGGACG CAACGTCTTG GAGCTCACCA ACATCCGTCA GTCGACCAAC 
TACACCTGCG TGGCTATATC ATCCCTAGGT ATGATCGAGG CAACGGCACA AGTGTCTGTC 
AAAGCTCTCC CAAAGCCTCC TACATCCCTG ACTGTTACGG AAACCACAGC TACGAGTGTT 
ACACTCACAT GGGACTCTGG AAACCCTGAA CCTGTGTCTT ACTACATAAT TCAATACCGG 
GCCAAGACTT CAGACACCAA CTTCCAAGAA GTGGACGGTG TGGCCACCAC ACGTTACAGC 
ATCGGCGGTC TCAGCCCATA TTCAGAGTAT GAGTTTCGAG TAATGGCAGT CAACAACATT 
GGTCGTGGGC CACCTAGTGA ACCCGTTGAA ACACGAACAG GTGAACAGGC ACCTTCCTCC 
CCACCGCTGC ATGTTCAAGC ACGAATGCTG AGTGCCAGCA CCATGTTGGT TCAGTGGGAA 
CCACCAGAGG AACCCAATGG TCAGATCAGA GGCTATCGGA TCTACTACAC ATCTGACCTG 
GATGCACCTC TTAGCGCCTG GCAGAAACAC AACACAGACG ATAGCCGTCT CACCACTATC 
TCCAGCCTGA CCACCGGCAT CACCTACAGC CTACGTGTGC TGGGCTTCAC ATCTGTAGGG 
GATGGACCAC CATCTGATGT GCTACAGGTC AAGACACAGC AAGGAGTTCC TGCTCAGCCA 
TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT ACAAGGATCC TACTAACTTG GCTGTGGCCC 
GTGCAGGACC CCATCATCAA ATACGAGCTG CAATATTGGG AGGCTGACTC AGACAACAAG 
ATCCATGTCA CCTTTGACCC AGCTGGCTCG TACGCTGTTG AAGGTCTGAA ACCAGACACT 
TTGTACAAAT TCTCTCTGGC AGCCCGCTCT GAAATGGGAC AAGGAGTCTT CACTCACCCC 
ATTGAAGCCA GGACGGCCCA GTCTGCCCCC TCTGCCCCCC CTCAAGACGT GCACCTGCTC 
AGTCTGAGCT CCACCAGCAT CAAGGTGAGT TGGGTGGCAC CACCCGCCGC TAGCCGCCAC 
GGCGCAATAG TGCGCTACAC AGTCAGCTAC CAGGCAGTGA ATGGAGAAGA CACAGAGCGC 
CATGAGGTGC CGGACATTGG AGCGGATGCC TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG 
TGGACGGAGT ATCAGGTGTG GGTGAGAGCG CACACTGATG TGGGCCCCGG CCCCGAGAGC 
ACGGCTGTGC GTATCCGAAC CAATGAAGAC GTGCCAGGAG CACCGCCTCG GAAATTGGAA 
GTGGAGGCTT TGAACTCCAC CGCTATCCGT GTGACATGGA AACCTCCTCT TTCTGGAAAG 
CAGCATGGCC AGATCCGGGG ATATCAGGTC ATATACTCCC GGTTGGAGAA CGGGGAACCA 
CGGGGACATC CGAATATAAT GGATGTCGCT CTGCCAGAAG CACAGTGGAA AATTGAAGAG 
TCTACGGAAT ATGAGGCTGT GATCGCTGGA CTTGCCTCTG AAACCTCTTA CTCAGTGACT 
GTGGCTGCGT ACACAACCAA AGGTGATGGA GCTCGCAGCA AAGCCAAAGT GGTCACCACC 
ACCGGAGCAG TGCCTGGCAA ACCCACTATG ATCATCAGCA CCACCGTTGG CAACACGGCC 
CTTATCCAGT GGCAGCCTCC TAAAGACATG GTGGGTGAGC TGATGGGCTA CCGGCTGCGC 
TACAAACGTC TGGAAGAGGA AAACTATGAG ATACGCGAAT TCAGTCGCAC CGACGACCAC 
CACACCGTCA CTGACCTGCA CAAGGGCGCC ACCTACAGCT TCCACCTGAG CGCCAGAAAC 
CGCGCTGGAC TCGGAGAGGA GTACGTTAAA GACATCGGCA CTCCTGAGGA CGTGCCCAGC 
GGGTTTCCTC TTAACCTGCA GGTCGTAGGC TTGACTACCT CTACCACCCG ACTGACCTGG 
GAGCCTCCAG CGTTGTCTGA ACGTAACGGA CGGATCATAC ATTACATTGT GGTTTACCGA 
GACATCAACA GCAAACAAAA CAGCACCAAC CGAACCTCCG ACACACAGAT GACCATCCAA 
GGGCTCCGAC CTGACACCAC CTATGATATC CGTGTGCAGG CGTTCACTAG CAAGGGTGGA 
GGTCCCATAA GTCCCAGTAT CCAGAGCAGG ACCATGTCTA CGTCACCTGC ATTTGCCACA 
AGTTTTGGTG TCAAAGCTGT GATGAAAACC TCTGTCCTTC TCACCTGGGA GGTGCCAGAA 
AATTATAAAT CACAAGTGCC TTTTAAGATC CTCTATAACC AGCAGAGTGT GGAAGTCCAG 
GGGAATCTGA AGAGGAAGCT GATCACACGC CTGCAGCCGG ACACTGATTA CTCGTTTGTG 
CTGATGAGCC GGGGAAACAG CGCCGGTGGC CTACAACAGC AGGTCTCCAT CCGCACTGCA 
CCAGACCTGT TCAAAACTAA ACCTGTCCTG TACACGGCAG ACCAGACCAA CAATGGCAAA 
CTCACCATCA ACCTGCCCAA AGTGCCCACC TCTGCTCCTG TCAGGTGTTA TTACATTGTG 
GTCGTGCCCG TCTCTCAGAC ATCATCTCGT CAGTGGATTA ATCCCGATGA GATGGAGCTG 
GATGAGCTCC TGGAGTCTAG TGAGGGAGCT CATCTCAGGA GACGTCGACG GCACACAGAC 
ACCGTAAGAC CGTACATCGC CGCAAAGCTA TCCACGCTCC CAGAAACCTT CACTCTGGGC 
GATGAGCTAG ACTACAGCGG CTTCATCAAC CGGCCTCTTC CCAACAACCA ACACTTCCAG 
TGTTTTGTGA TGGCTGAACT CAAGGACCAA TACCCTGTTA CTGCCAATGA GAAGCAGCGG 
ACCTTCTCGG CTAGCCCATA CTCAGACCCC ATCATAGTGC AGGGTGAAAA CCAAATGCAG 
CGCAGTGTGG ACCAACCGGA GATGCTTTGG GTCATGGGCC CTGTGCTCGC TGTGGTGCTC 
ATCATCATCA TAGTCATCGC CATCCTGCTC TTCAAAAGCA AACAGGAGAG GAAGAGGGCT 
TCCCCCCTAC CAAAAGACGA TCATTCGGGT GGTGTGAAGG ATTGTCTTCT GGCCAGCTCA 
TCCGATCCTG TCGAAATGAG GAGACTCAAC TACCAGACAC CAGGAATGAG GGAACATCCT 
CCCATATCCG TCTCCGAACT GGCAGACCAC ATCGAGCGCC TCAAGGCCAA CGATGCCCTC 
CGGTTTTCCC AAGAGTATGA GTCTATTGAT CCAGGCCAGC AGTTCACATG GGAGAACTCC 
AACTTGGAGG TCAACAAGCC AAAGAACCGT TATGCCAACG TTATTGCTTA CGACCACTCG 
AGAGTTGTTC TCACCCCTGT GGACGGTGTA CCAGGGAGTG ACTACATTAA TTCCAACTAC 
ATTGATGGCT ACCGTAAGCA GAATGCCTAC ATTGCCACAC AAGGTCCTCT GCCTGAGACT 
CTGAGCGACT TCTGGAGGAT GATTTGGGAG CAACGAGCCA GTACCATCGT CATGATGACC 
AGACTGGAAG AGAAGTCCAG AGTCAAGTGT GACCAATACT GGCCCATTCG TGGCACAGAG 
ACTTATGGAA TGATTCAAGT GACCATGCTT GATGCTGTTG AGCTGGCAAC CTACAGTGTG 
CGCACCTTTG CCCTTTACAA GAATGGCTCT AGTGAGAAGC GCGAGGTCAG GCAGTTTCAG 
TTCATGGCTT GGCCGGACCA CGGAGTGCCT GAATATCCCA CCCCCATCCT AGCCTTCCTG 
CGCAGGGTCA AAGCCTGCAA CCCTCCTGAT GCTGGACCCA TGGTAGTGCA CTGCAGTGCT 
GGAGTTGGCC GTACAGGCTG CTTCATAGTC ATCGATGCGA TGTTGGAGCG CATGAAGCAC 
GAGAAGACCG TAGACATTTA TGGCCACGTC ACGTGCATGA GATCCCAGAG GAACTATATG 
GTGCAGACGG AGGACCAGTA CATCTTTATC CACGAGGCCC TTCTGGAGGC CGCCACCTGT 
GGAAACACAG AGGTCCCTGC CCGAAACCTC TACGCCCACA TCCAGAAGCT CACACAGCCA 
TCGGCTGGAG AGACCGTCAC AGCCATGGAG CTGGAGTTTA AGAGACTCGC CAACTCCAAA 
GCCCACACCT CAAGATTCAT CAGCGCCAAC CTGCCCTGCA ACAAGTTCAA GAACCGGCTG 
GTCAATATCA TGCCTTTCGA GTCCACACGG GTGTGCCTGC AGCCAATCCG AGGAGTGGAG 
GGGTCCGACT ACATCAATGC CAGCTGCATT GACGGATACA GACAGCAGAA GGCCTATATT 
GCCACTCAGG GTCCTCTAGC GGAGACCACC GAGGACTTCT GGAGGATGTT ATGGGAGCAC 
AATTCCACTA TTGTAGTGAT GCTGACCAAA CTCAGAGAGA TGGGACGGGA GAAGTGTCAT 
CAGTACTGGC CCGCCGAGCG CTCAGCCCGC TATCAGTACT TCGTAGTGGA TCCCATGGCC 
GAATACAACA TGCCCCAGTA CATCCTGCGA GAGTTCAAAG TCACAGATGC CAGGGACGGT 
CAATCCAGAA CCATTCGCCA GTTCCAGTTC ACTGATTGGC CAGAGCAGGG AGTGCCAAAA 
ACCGGTGAGG GGTTCATCGA TTTCATTGGG CAAGTTCATA AAACCAAGGA GCAGTTTGGG 
CAGGATGGAC CAATCACTGT GCACTGCAGT GCTGGAGTGG GAAGAACAGG TGTTTTCATC 
ACTCTCAGTA TTGTCCTGGA GAGGATGAGA TATGAGGGGG TGGTGGATCT GTTCCAGACG 
GTTAAAACCC TGCGCACACA AAGGCCTGCC ATGGTGCAGA CAGAGGACCA GTACCAGCTG 
TGCTACAGAG CCGCTCTGGA GTACCTGGGG AGCTTTGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_021481437.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337112.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X14, mRNA.

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CloneID	ODa150376
Clone ID Related Accession (Same CDS sequence)	XM_021481437.1
Accession Version	XM_021481437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TACGACGAGT TCCACCCCGT 
TTCTCCATCC CACCTACCAA TCACGAGGTG ATGCCAGGGG GCAGTGTAAA CCTCACCTGC 
GTGGCAGTAG GTGCCCCGAT GCCCTACGTC AAATGGATGA CTGGTGAGGT GGAGCTCACC 
AAGGATGAGG AGATGCCTGT GGGACGCAAC GTCTTGGAGC TCACCAACAT CCGTCAGTCG 
ACCAACTACA CCTGCGTGGC TATATCATCC CTAGGTATGA TCGAGGCAAC GGCACAAGTG 
TCTGTCAAAG CTCTCCCAAA GCCTCCTACA TCCCTGACTG TTACGGAAAC CACAGCTACG 
AGTGTTACAC TCACATGGGA CTCTGGAAAC CCTGAACCTG TGTCTTACTA CATAATTCAA 
TACCGGGCCA AGACTTCAGA CACCAACTTC CAAGAAGTGG ACGGTGTGGC CACCACACGT 
TACAGCATCG GCGGTCTCAG CCCATATTCA GAGTATGAGT TTCGAGTAAT GGCAGTCAAC 
AACATTGGTC GTGGGCCACC TAGTGAACCC GTTGAAACAC GAACAGGTGA ACAGGCACCT 
TCCTCCCCAC CGCTGCATGT TCAAGCACGA ATGCTGAGTG CCAGCACCAT GTTGGTTCAG 
TGGGAACCAC CAGAGGAACC CAATGGTCAG ATCAGAGGCT ATCGGATCTA CTACACATCT 
GACCTGGATG CACCTCTTAG CGCCTGGCAG AAACACAACA CAGACGATAG CCGTCTCACC 
ACTATCTCCA GCCTGACCAC CGGCATCACC TACAGCCTAC GTGTGCTGGG CTTCACATCT 
GTAGGGGATG GACCACCATC TGATGTGCTA CAGGTCAAGA CACAGCAAGG AGTTCCTGCT 
CAGCCATCCA GCTTCGAAGC TGAAGCAGAG CTAGATACAA GGATCCTACT AACTTGGCTG 
TGGCCCGTGC AGGACCCCAT CATCAAATAC GAGCTGCAAT ATTGGGAGGC TGACTCAGAC 
AACAAGATCC ATGTCACCTT TGACCCAGCT GGCTCGTACG CTGTTGAAGG TCTGAAACCA 
GACACTTTGT ACAAATTCTC TCTGGCAGCC CGCTCTGAAA TGGGACAAGG AGTCTTCACT 
CACCCCATTG AAGCCAGGAC GGCCCAGTCT GTGCCAGGAG CACCGCCTCG GAAATTGGAA 
GTGGAGGCTT TGAACTCCAC CGCTATCCGT GTGACATGGA AACCTCCTCT TTCTGGAAAG 
CAGCATGGCC AGATCCGGGG ATATCAGGTC ATATACTCCC GGTTGGAGAA CGGGGAACCA 
CGGGGACATC CGAATATAAT GGATGTCGCT CTGCCAGAAG CACAGTGGAA AATTGAAGAG 
TCTACGGAAT ATGAGGCTGT GATCGCTGGA CTTGCCTCTG AAACCTCTTA CTCAGTGACT 
GTGGCTGCGT ACACAACCAA AGGTGATGGA GCTCGCAGCA AAGCCAAAGT GGTCACCACC 
ACCGGAGCAG TGCCTGGCAA ACCCACTATG ATCATCAGCA CCACCGTTGG CAACACGGCC 
CTTATCCAGT GGCAGCCTCC TAAAGACATG GTGGGTGAGC TGATGGGCTA CCGGCTGCGC 
TACAAACGTC TGGAAGAGGA AAACTATGAG ATACGCGAAT TCAGTCGCAC CGACGACCAC 
CACACCGTCA CTGACCTGCA CAAGGGCGCC ACCTACAGCT TCCACCTGAG CGCCAGAAAC 
CGCGCTGGAC TCGGAGAGGA GTACGTTAAA GACATCGGCA CTCCTGAGGA CGTGCCCAGC 
GGGTTTCCTC TTAACCTGCA GGTCGTAGGC TTGACTACCT CTACCACCCG ACTGACCTGG 
GAGCCTCCAG CGTTGTCTGA ACGTAACGGA CGGATCATAC ATTACATTGT GGTTTACCGA 
GACATCAACA GCAAACAAAA CAGCACCAAC CGAACCTCCG ACACACAGAT GACCATCCAA 
GGGCTCCGAC CTGACACCAC CTATGATATC CGTGTGCAGG CGTTCACTAG CAAGGGTGGA 
GGTCCCATAA GTCCCAGTAT CCAGAGCAGG ACCATGTCTA CGTCACCTGC ATTTGCCACA 
AGTTTTGGTG TCAAAGCTGT GATGAAAACC TCTGTCCTTC TCACCTGGGA GGTGCCAGAA 
AATTATAAAT CACAAGTGCC TTTTAAGATC CTCTATAACC AGCAGAGTGT GGAAGTCCAG 
GGGAATCTGA AGAGGAAGCT GATCACACGC CTGCAGCCGG ACACTGATTA CTCGTTTGTG 
CTGATGAGCC GGGGAAACAG CGCCGGTGGC CTACAACAGC AGGTCTCCAT CCGCACTGCA 
CCAGACCTGT TCAAAACTAA ACCTGTCCTG TACACGGCAG ACCAGACCAA CAATGGCAAA 
CTCACCATCA ACCTGCCCAA AGTGCCCACC TCTGCTCCTG TCAGGTGTTA TTACATTGTG 
GTCGTGCCCG TCTCTCAGAC ATCATCTCGT CAGTGGATTA ATCCCGATGA GATGGAGCTG 
GATGAGCTCC TGGAGTCTAG TGAGGGAGCT CATCTCAGGA GACGTCGACG GCACACAGAC 
ACCGTAAGAC CGTACATCGC CGCAAAGCTA TCCACGCTCC CAGAAACCTT CACTCTGGGC 
GATGAGCTAG ACTACAGCGG CTTCATCAAC CGGCCTCTTC CCAACAACCA ACACTTCCAG 
TGTTTTGTGA TGGCTGAACT CAAGGACCAA TACCCTGTTA CTGCCAATGA GAAGCAGCGG 
ACCTTCTCGG CTAGCCCATA CTCAGACCCC ATCATAGTGC AGGGTGAAAA CCAAATGCAG 
CGCAGTGTGG ACCAACCGGA GATGCTTTGG GTCATGGGCC CTGTGCTCGC TGTGGTGCTC 
ATCATCATCA TAGTCATCGC CATCCTGCTC TTCAAAAGCA AACAGGAGAG GAAGAGGGCT 
TCCCCCCTAC CAAAAGACGA TCATTCGGGT GGTGTGAAGG ATTGTCTTCT GGCCAGCTCA 
TCCGATCCTG TCGAAATGAG GAGACTCAAC TACCAGACAC CAGGAATGAG GGAACATCCT 
CCCATATCCG TCTCCGAACT GGCAGACCAC ATCGAGCGCC TCAAGGCCAA CGATGCCCTC 
CGGTTTTCCC AAGAGTATGA GTCTATTGAT CCAGGCCAGC AGTTCACATG GGAGAACTCC 
AACTTGGAGG TCAACAAGCC AAAGAACCGT TATGCCAACG TTATTGCTTA CGACCACTCG 
AGAGTTGTTC TCACCCCTGT GGACGGTGTA CCAGGGAGTG ACTACATTAA TTCCAACTAC 
ATTGATGGCT ACCGTAAGCA GAATGCCTAC ATTGCCACAC AAGGTCCTCT GCCTGAGACT 
CTGAGCGACT TCTGGAGGAT GATTTGGGAG CAACGAGCCA GTACCATCGT CATGATGACC 
AGACTGGAAG AGAAGTCCAG AGTCAAGTGT GACCAATACT GGCCCATTCG TGGCACAGAG 
ACTTATGGAA TGATTCAAGT GACCATGCTT GATGCTGTTG AGCTGGCAAC CTACAGTGTG 
CGCACCTTTG CCCTTTACAA GAATGGCTCT AGTGAGAAGC GCGAGGTCAG GCAGTTTCAG 
TTCATGGCTT GGCCGGACCA CGGAGTGCCT GAATATCCCA CCCCCATCCT AGCCTTCCTG 
CGCAGGGTCA AAGCCTGCAA CCCTCCTGAT GCTGGACCCA TGGTAGTGCA CTGCAGTGCT 
GGAGTTGGCC GTACAGGCTG CTTCATAGTC ATCGATGCGA TGTTGGAGCG CATGAAGCAC 
GAGAAGACCG TAGACATTTA TGGCCACGTC ACGTGCATGA GATCCCAGAG GAACTATATG 
GTGCAGACGG AGGACCAGTA CATCTTTATC CACGAGGCCC TTCTGGAGGC CGCCACCTGT 
GGAAACACAG AGGTCCCTGC CCGAAACCTC TACGCCCACA TCCAGAAGCT CACACAGCCA 
TCGGCTGGAG AGACCGTCAC AGCCATGGAG CTGGAGTTTA AGAGACTCGC CAACTCCAAA 
GCCCACACCT CAAGATTCAT CAGCGCCAAC CTGCCCTGCA ACAAGTTCAA GAACCGGCTG 
GTCAATATCA TGCCTTTCGA GTCCACACGG GTGTGCCTGC AGCCAATCCG AGGAGTGGAG 
GGGTCCGACT ACATCAATGC CAGCTGCATT GACGGATACA GACAGCAGAA GGCCTATATT 
GCCACTCAGG GTCCTCTAGC GGAGACCACC GAGGACTTCT GGAGGATGTT ATGGGAGCAC 
AATTCCACTA TTGTAGTGAT GCTGACCAAA CTCAGAGAGA TGGGACGGGA GAAGTGTCAT 
CAGTACTGGC CCGCCGAGCG CTCAGCCCGC TATCAGTACT TCGTAGTGGA TCCCATGGCC 
GAATACAACA TGCCCCAGTA CATCCTGCGA GAGTTCAAAG TCACAGATGC CAGGGACGGT 
CAATCCAGAA CCATTCGCCA GTTCCAGTTC ACTGATTGGC CAGAGCAGGG AGTGCCAAAA 
ACCGGTGAGG GGTTCATCGA TTTCATTGGG CAAGTTCATA AAACCAAGGA GCAGTTTGGG 
CAGGATGGAC CAATCACTGT GCACTGCAGT GCTGGAGTGG GAAGAACAGG TGTTTTCATC 
ACTCTCAGTA TTGTCCTGGA GAGGATGAGA TATGAGGGGG TGGTGGATCT GTTCCAGACG 
GTTAAAACCC TGCGCACACA AAGGCCTGCC ATGGTGCAGA CAGAGGACCA GTACCAGCTG 
TGCTACAGAG CCGCTCTGGA GTACCTGGGG AGCTTTGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337112.1
CDS	421..5874
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021481437.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481437.1

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TACGACGAGT TCCACCCCGT 
TTCTCCATCC CACCTACCAA TCACGAGGTG ATGCCAGGGG GCAGTGTAAA CCTCACCTGC 
GTGGCAGTAG GTGCCCCGAT GCCCTACGTC AAATGGATGA CTGGTGAGGT GGAGCTCACC 
AAGGATGAGG AGATGCCTGT GGGACGCAAC GTCTTGGAGC TCACCAACAT CCGTCAGTCG 
ACCAACTACA CCTGCGTGGC TATATCATCC CTAGGTATGA TCGAGGCAAC GGCACAAGTG 
TCTGTCAAAG CTCTCCCAAA GCCTCCTACA TCCCTGACTG TTACGGAAAC CACAGCTACG 
AGTGTTACAC TCACATGGGA CTCTGGAAAC CCTGAACCTG TGTCTTACTA CATAATTCAA 
TACCGGGCCA AGACTTCAGA CACCAACTTC CAAGAAGTGG ACGGTGTGGC CACCACACGT 
TACAGCATCG GCGGTCTCAG CCCATATTCA GAGTATGAGT TTCGAGTAAT GGCAGTCAAC 
AACATTGGTC GTGGGCCACC TAGTGAACCC GTTGAAACAC GAACAGGTGA ACAGGCACCT 
TCCTCCCCAC CGCTGCATGT TCAAGCACGA ATGCTGAGTG CCAGCACCAT GTTGGTTCAG 
TGGGAACCAC CAGAGGAACC CAATGGTCAG ATCAGAGGCT ATCGGATCTA CTACACATCT 
GACCTGGATG CACCTCTTAG CGCCTGGCAG AAACACAACA CAGACGATAG CCGTCTCACC 
ACTATCTCCA GCCTGACCAC CGGCATCACC TACAGCCTAC GTGTGCTGGG CTTCACATCT 
GTAGGGGATG GACCACCATC TGATGTGCTA CAGGTCAAGA CACAGCAAGG AGTTCCTGCT 
CAGCCATCCA GCTTCGAAGC TGAAGCAGAG CTAGATACAA GGATCCTACT AACTTGGCTG 
TGGCCCGTGC AGGACCCCAT CATCAAATAC GAGCTGCAAT ATTGGGAGGC TGACTCAGAC 
AACAAGATCC ATGTCACCTT TGACCCAGCT GGCTCGTACG CTGTTGAAGG TCTGAAACCA 
GACACTTTGT ACAAATTCTC TCTGGCAGCC CGCTCTGAAA TGGGACAAGG AGTCTTCACT 
CACCCCATTG AAGCCAGGAC GGCCCAGTCT GTGCCAGGAG CACCGCCTCG GAAATTGGAA 
GTGGAGGCTT TGAACTCCAC CGCTATCCGT GTGACATGGA AACCTCCTCT TTCTGGAAAG 
CAGCATGGCC AGATCCGGGG ATATCAGGTC ATATACTCCC GGTTGGAGAA CGGGGAACCA 
CGGGGACATC CGAATATAAT GGATGTCGCT CTGCCAGAAG CACAGTGGAA AATTGAAGAG 
TCTACGGAAT ATGAGGCTGT GATCGCTGGA CTTGCCTCTG AAACCTCTTA CTCAGTGACT 
GTGGCTGCGT ACACAACCAA AGGTGATGGA GCTCGCAGCA AAGCCAAAGT GGTCACCACC 
ACCGGAGCAG TGCCTGGCAA ACCCACTATG ATCATCAGCA CCACCGTTGG CAACACGGCC 
CTTATCCAGT GGCAGCCTCC TAAAGACATG GTGGGTGAGC TGATGGGCTA CCGGCTGCGC 
TACAAACGTC TGGAAGAGGA AAACTATGAG ATACGCGAAT TCAGTCGCAC CGACGACCAC 
CACACCGTCA CTGACCTGCA CAAGGGCGCC ACCTACAGCT TCCACCTGAG CGCCAGAAAC 
CGCGCTGGAC TCGGAGAGGA GTACGTTAAA GACATCGGCA CTCCTGAGGA CGTGCCCAGC 
GGGTTTCCTC TTAACCTGCA GGTCGTAGGC TTGACTACCT CTACCACCCG ACTGACCTGG 
GAGCCTCCAG CGTTGTCTGA ACGTAACGGA CGGATCATAC ATTACATTGT GGTTTACCGA 
GACATCAACA GCAAACAAAA CAGCACCAAC CGAACCTCCG ACACACAGAT GACCATCCAA 
GGGCTCCGAC CTGACACCAC CTATGATATC CGTGTGCAGG CGTTCACTAG CAAGGGTGGA 
GGTCCCATAA GTCCCAGTAT CCAGAGCAGG ACCATGTCTA CGTCACCTGC ATTTGCCACA 
AGTTTTGGTG TCAAAGCTGT GATGAAAACC TCTGTCCTTC TCACCTGGGA GGTGCCAGAA 
AATTATAAAT CACAAGTGCC TTTTAAGATC CTCTATAACC AGCAGAGTGT GGAAGTCCAG 
GGGAATCTGA AGAGGAAGCT GATCACACGC CTGCAGCCGG ACACTGATTA CTCGTTTGTG 
CTGATGAGCC GGGGAAACAG CGCCGGTGGC CTACAACAGC AGGTCTCCAT CCGCACTGCA 
CCAGACCTGT TCAAAACTAA ACCTGTCCTG TACACGGCAG ACCAGACCAA CAATGGCAAA 
CTCACCATCA ACCTGCCCAA AGTGCCCACC TCTGCTCCTG TCAGGTGTTA TTACATTGTG 
GTCGTGCCCG TCTCTCAGAC ATCATCTCGT CAGTGGATTA ATCCCGATGA GATGGAGCTG 
GATGAGCTCC TGGAGTCTAG TGAGGGAGCT CATCTCAGGA GACGTCGACG GCACACAGAC 
ACCGTAAGAC CGTACATCGC CGCAAAGCTA TCCACGCTCC CAGAAACCTT CACTCTGGGC 
GATGAGCTAG ACTACAGCGG CTTCATCAAC CGGCCTCTTC CCAACAACCA ACACTTCCAG 
TGTTTTGTGA TGGCTGAACT CAAGGACCAA TACCCTGTTA CTGCCAATGA GAAGCAGCGG 
ACCTTCTCGG CTAGCCCATA CTCAGACCCC ATCATAGTGC AGGGTGAAAA CCAAATGCAG 
CGCAGTGTGG ACCAACCGGA GATGCTTTGG GTCATGGGCC CTGTGCTCGC TGTGGTGCTC 
ATCATCATCA TAGTCATCGC CATCCTGCTC TTCAAAAGCA AACAGGAGAG GAAGAGGGCT 
TCCCCCCTAC CAAAAGACGA TCATTCGGGT GGTGTGAAGG ATTGTCTTCT GGCCAGCTCA 
TCCGATCCTG TCGAAATGAG GAGACTCAAC TACCAGACAC CAGGAATGAG GGAACATCCT 
CCCATATCCG TCTCCGAACT GGCAGACCAC ATCGAGCGCC TCAAGGCCAA CGATGCCCTC 
CGGTTTTCCC AAGAGTATGA GTCTATTGAT CCAGGCCAGC AGTTCACATG GGAGAACTCC 
AACTTGGAGG TCAACAAGCC AAAGAACCGT TATGCCAACG TTATTGCTTA CGACCACTCG 
AGAGTTGTTC TCACCCCTGT GGACGGTGTA CCAGGGAGTG ACTACATTAA TTCCAACTAC 
ATTGATGGCT ACCGTAAGCA GAATGCCTAC ATTGCCACAC AAGGTCCTCT GCCTGAGACT 
CTGAGCGACT TCTGGAGGAT GATTTGGGAG CAACGAGCCA GTACCATCGT CATGATGACC 
AGACTGGAAG AGAAGTCCAG AGTCAAGTGT GACCAATACT GGCCCATTCG TGGCACAGAG 
ACTTATGGAA TGATTCAAGT GACCATGCTT GATGCTGTTG AGCTGGCAAC CTACAGTGTG 
CGCACCTTTG CCCTTTACAA GAATGGCTCT AGTGAGAAGC GCGAGGTCAG GCAGTTTCAG 
TTCATGGCTT GGCCGGACCA CGGAGTGCCT GAATATCCCA CCCCCATCCT AGCCTTCCTG 
CGCAGGGTCA AAGCCTGCAA CCCTCCTGAT GCTGGACCCA TGGTAGTGCA CTGCAGTGCT 
GGAGTTGGCC GTACAGGCTG CTTCATAGTC ATCGATGCGA TGTTGGAGCG CATGAAGCAC 
GAGAAGACCG TAGACATTTA TGGCCACGTC ACGTGCATGA GATCCCAGAG GAACTATATG 
GTGCAGACGG AGGACCAGTA CATCTTTATC CACGAGGCCC TTCTGGAGGC CGCCACCTGT 
GGAAACACAG AGGTCCCTGC CCGAAACCTC TACGCCCACA TCCAGAAGCT CACACAGCCA 
TCGGCTGGAG AGACCGTCAC AGCCATGGAG CTGGAGTTTA AGAGACTCGC CAACTCCAAA 
GCCCACACCT CAAGATTCAT CAGCGCCAAC CTGCCCTGCA ACAAGTTCAA GAACCGGCTG 
GTCAATATCA TGCCTTTCGA GTCCACACGG GTGTGCCTGC AGCCAATCCG AGGAGTGGAG 
GGGTCCGACT ACATCAATGC CAGCTGCATT GACGGATACA GACAGCAGAA GGCCTATATT 
GCCACTCAGG GTCCTCTAGC GGAGACCACC GAGGACTTCT GGAGGATGTT ATGGGAGCAC 
AATTCCACTA TTGTAGTGAT GCTGACCAAA CTCAGAGAGA TGGGACGGGA GAAGTGTCAT 
CAGTACTGGC CCGCCGAGCG CTCAGCCCGC TATCAGTACT TCGTAGTGGA TCCCATGGCC 
GAATACAACA TGCCCCAGTA CATCCTGCGA GAGTTCAAAG TCACAGATGC CAGGGACGGT 
CAATCCAGAA CCATTCGCCA GTTCCAGTTC ACTGATTGGC CAGAGCAGGG AGTGCCAAAA 
ACCGGTGAGG GGTTCATCGA TTTCATTGGG CAAGTTCATA AAACCAAGGA GCAGTTTGGG 
CAGGATGGAC CAATCACTGT GCACTGCAGT GCTGGAGTGG GAAGAACAGG TGTTTTCATC 
ACTCTCAGTA TTGTCCTGGA GAGGATGAGA TATGAGGGGG TGGTGGATCT GTTCCAGACG 
GTTAAAACCC TGCGCACACA AAGGCCTGCC ATGGTGCAGA CAGAGGACCA GTACCAGCTG 
TGCTACAGAG CCGCTCTGGA GTACCTGGGG AGCTTTGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_021481438.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337113.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X15, mRNA.

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CloneID	ODa150377
Clone ID Related Accession (Same CDS sequence)	XM_021481438.1
Accession Version	XM_021481438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGTGCCAGG AGCACCGCCT CGGAAATTGG AAGTGGAGGC TTTGAACTCC 
ACCGCTATCC GTGTGACATG GAAACCTCCT CTTTCTGGAA AGCAGCATGG CCAGATCCGG 
GGATATCAGG TCATATACTC CCGGTTGGAG AACGGGGAAC CACGGGGACA TCCGAATATA 
ATGGATGTCG CTCTGCCAGA AGCACAGTGG AAAATTGAAG AGTCTACGGA ATATGAGGCT 
GTGATCGCTG GACTTGCCTC TGAAACCTCT TACTCAGTGA CTGTGGCTGC GTACACAACC 
AAAGGTGATG GAGCTCGCAG CAAAGCCAAA GTGGTCACCA CCACCGGAGC AGTGCCTGGC 
AAACCCACTA TGATCATCAG CACCACCGTT GGCAACACGG CCCTTATCCA GTGGCAGCCT 
CCTAAAGACA TGGTGGGTGA GCTGATGGGC TACCGGCTGC GCTACAAACG TCTGGAAGAG 
GAAAACTATG AGATACGCGA ATTCAGTCGC ACCGACGACC ACCACACCGT CACTGACCTG 
CACAAGGGCG CCACCTACAG CTTCCACCTG AGCGCCAGAA ACCGCGCTGG ACTCGGAGAG 
GAGTACGTTA AAGACATCGG CACTCCTGAG GACGTGCCCA GCGGGTTTCC TCTTAACCTG 
CAGGTCGTAG GCTTGACTAC CTCTACCACC CGACTGACCT GGGAGCCTCC AGCGTTGTCT 
GAACGTAACG GACGGATCAT ACATTACATT GTGGTTTACC GAGACATCAA CAGCAAACAA 
AACAGCACCA ACCGAACCTC CGACACACAG ATGACCATCC AAGGGCTCCG ACCTGACACC 
ACCTATGATA TCCGTGTGCA GGCGTTCACT AGCAAGGGTG GAGGTCCCAT AAGTCCCAGT 
ATCCAGAGCA GGACCATGTC TACGTCACCT GCATTTGCCA CAAGTTTTGG TGTCAAAGCT 
GTGATGAAAA CCTCTGTCCT TCTCACCTGG GAGGTGCCAG AAAATTATAA ATCACAAGTG 
CCTTTTAAGA TCCTCTATAA CCAGCAGAGT GTGGAAGTCC AGGGGAATCT GAAGAGGAAG 
CTGATCACAC GCCTGCAGCC GGACACTGAT TACTCGTTTG TGCTGATGAG CCGGGGAAAC 
AGCGCCGGTG GCCTACAACA GCAGGTCTCC ATCCGCACTG CACCAGACCT GTTCAAAACT 
AAACCTGTCC TGTACACGGC AGACCAGACC AACAATGGCA AACTCACCAT CAACCTGCCC 
AAAGTGCCCA CCTCTGCTCC TGTCAGGTGT TATTACATTG TGGTCGTGCC CGTCTCTCAG 
ACATCATCTC GTCAGTGGAT TAATCCCGAT GAGATGGAGC TGGATGAGCT CCTGGAGTCT 
AGTGAGGGAG CTCATCTCAG GAGACGTCGA CGGCACACAG ACACCGTAAG ACCGTACATC 
GCCGCAAAGC TATCCACGCT CCCAGAAACC TTCACTCTGG GCGATGAGCT AGACTACAGC 
GGCTTCATCA ACCGGCCTCT TCCCAACAAC CAACACTTCC AGTGTTTTGT GATGGCTGAA 
CTCAAGGACC AATACCCTGT TACTGCCAAT GAGAAGCAGC GGACCTTCTC GGCTAGCCCA 
TACTCAGACC CCATCATAGT GCAGGGTGAA AACCAAATGC AGCGCAGTGT GGACCAACCG 
GAGATGCTTT GGGTCATGGG CCCTGTGCTC GCTGTGGTGC TCATCATCAT CATAGTCATC 
GCCATCCTGC TCTTCAAAAG CAAACAGGAG AGGAAGAGGG CTTCCCCCCT ACCAAAAGAC 
GATCATTCGG GTGGTGTGAA GGATTGTCTT CTGGCCAGCT CATCCGATCC TGTCGAAATG 
AGGAGACTCA ACTACCAGAC ACCAGGAATG AGGGAACATC CTCCCATATC CGTCTCCGAA 
CTGGCAGACC ACATCGAGCG CCTCAAGGCC AACGATGCCC TCCGGTTTTC CCAAGAGTAT 
GAGTCTATTG ATCCAGGCCA GCAGTTCACA TGGGAGAACT CCAACTTGGA GGTCAACAAG 
CCAAAGAACC GTTATGCCAA CGTTATTGCT TACGACCACT CGAGAGTTGT TCTCACCCCT 
GTGGACGGTG TACCAGGGAG TGACTACATT AATTCCAACT ACATTGATGG CTACCGTAAG 
CAGAATGCCT ACATTGCCAC ACAAGGTCCT CTGCCTGAGA CTCTGAGCGA CTTCTGGAGG 
ATGATTTGGG AGCAACGAGC CAGTACCATC GTCATGATGA CCAGACTGGA AGAGAAGTCC 
AGAGTCAAGT GTGACCAATA CTGGCCCATT CGTGGCACAG AGACTTATGG AATGATTCAA 
GTGACCATGC TTGATGCTGT TGAGCTGGCA ACCTACAGTG TGCGCACCTT TGCCCTTTAC 
AAGAATGGCT CTAGTGAGAA GCGCGAGGTC AGGCAGTTTC AGTTCATGGC TTGGCCGGAC 
CACGGAGTGC CTGAATATCC CACCCCCATC CTAGCCTTCC TGCGCAGGGT CAAAGCCTGC 
AACCCTCCTG ATGCTGGACC CATGGTAGTG CACTGCAGTG CTGGAGTTGG CCGTACAGGC 
TGCTTCATAG TCATCGATGC GATGTTGGAG CGCATGAAGC ACGAGAAGAC CGTAGACATT 
TATGGCCACG TCACGTGCAT GAGATCCCAG AGGAACTATA TGGTGCAGAC GGAGGACCAG 
TACATCTTTA TCCACGAGGC CCTTCTGGAG GCCGCCACCT GTGGAAACAC AGAGGTCCCT 
GCCCGAAACC TCTACGCCCA CATCCAGAAG CTCACACAGC CATCGGCTGG AGAGACCGTC 
ACAGCCATGG AGCTGGAGTT TAAGAGACTC GCCAACTCCA AAGCCCACAC CTCAAGATTC 
ATCAGCGCCA ACCTGCCCTG CAACAAGTTC AAGAACCGGC TGGTCAATAT CATGCCTTTC 
GAGTCCACAC GGGTGTGCCT GCAGCCAATC CGAGGAGTGG AGGGGTCCGA CTACATCAAT 
GCCAGCTGCA TTGACGGATA CAGACAGCAG AAGGCCTATA TTGCCACTCA GGGTCCTCTA 
GCGGAGACCA CCGAGGACTT CTGGAGGATG TTATGGGAGC ACAATTCCAC TATTGTAGTG 
ATGCTGACCA AACTCAGAGA GATGGGACGG GAGAAGTGTC ATCAGTACTG GCCCGCCGAG 
CGCTCAGCCC GCTATCAGTA CTTCGTAGTG GATCCCATGG CCGAATACAA CATGCCCCAG 
TACATCCTGC GAGAGTTCAA AGTCACAGAT GCCAGGGACG GTCAATCCAG AACCATTCGC 
CAGTTCCAGT TCACTGATTG GCCAGAGCAG GGAGTGCCAA AAACCGGTGA GGGGTTCATC 
GATTTCATTG GGCAAGTTCA TAAAACCAAG GAGCAGTTTG GGCAGGATGG ACCAATCACT 
GTGCACTGCA GTGCTGGAGT GGGAAGAACA GGTGTTTTCA TCACTCTCAG TATTGTCCTG 
GAGAGGATGA GATATGAGGG GGTGGTGGAT CTGTTCCAGA CGGTTAAAAC CCTGCGCACA 
CAAAGGCCTG CCATGGTGCA GACAGAGGAC CAGTACCAGC TGTGCTACAG AGCCGCTCTG 
GAGTACCTGG GGAGCTTTGA TCACTATGCA ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337113.1
CDS	421..5856
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021481438.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481438.1

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGTGCCAGG AGCACCGCCT CGGAAATTGG AAGTGGAGGC TTTGAACTCC 
ACCGCTATCC GTGTGACATG GAAACCTCCT CTTTCTGGAA AGCAGCATGG CCAGATCCGG 
GGATATCAGG TCATATACTC CCGGTTGGAG AACGGGGAAC CACGGGGACA TCCGAATATA 
ATGGATGTCG CTCTGCCAGA AGCACAGTGG AAAATTGAAG AGTCTACGGA ATATGAGGCT 
GTGATCGCTG GACTTGCCTC TGAAACCTCT TACTCAGTGA CTGTGGCTGC GTACACAACC 
AAAGGTGATG GAGCTCGCAG CAAAGCCAAA GTGGTCACCA CCACCGGAGC AGTGCCTGGC 
AAACCCACTA TGATCATCAG CACCACCGTT GGCAACACGG CCCTTATCCA GTGGCAGCCT 
CCTAAAGACA TGGTGGGTGA GCTGATGGGC TACCGGCTGC GCTACAAACG TCTGGAAGAG 
GAAAACTATG AGATACGCGA ATTCAGTCGC ACCGACGACC ACCACACCGT CACTGACCTG 
CACAAGGGCG CCACCTACAG CTTCCACCTG AGCGCCAGAA ACCGCGCTGG ACTCGGAGAG 
GAGTACGTTA AAGACATCGG CACTCCTGAG GACGTGCCCA GCGGGTTTCC TCTTAACCTG 
CAGGTCGTAG GCTTGACTAC CTCTACCACC CGACTGACCT GGGAGCCTCC AGCGTTGTCT 
GAACGTAACG GACGGATCAT ACATTACATT GTGGTTTACC GAGACATCAA CAGCAAACAA 
AACAGCACCA ACCGAACCTC CGACACACAG ATGACCATCC AAGGGCTCCG ACCTGACACC 
ACCTATGATA TCCGTGTGCA GGCGTTCACT AGCAAGGGTG GAGGTCCCAT AAGTCCCAGT 
ATCCAGAGCA GGACCATGTC TACGTCACCT GCATTTGCCA CAAGTTTTGG TGTCAAAGCT 
GTGATGAAAA CCTCTGTCCT TCTCACCTGG GAGGTGCCAG AAAATTATAA ATCACAAGTG 
CCTTTTAAGA TCCTCTATAA CCAGCAGAGT GTGGAAGTCC AGGGGAATCT GAAGAGGAAG 
CTGATCACAC GCCTGCAGCC GGACACTGAT TACTCGTTTG TGCTGATGAG CCGGGGAAAC 
AGCGCCGGTG GCCTACAACA GCAGGTCTCC ATCCGCACTG CACCAGACCT GTTCAAAACT 
AAACCTGTCC TGTACACGGC AGACCAGACC AACAATGGCA AACTCACCAT CAACCTGCCC 
AAAGTGCCCA CCTCTGCTCC TGTCAGGTGT TATTACATTG TGGTCGTGCC CGTCTCTCAG 
ACATCATCTC GTCAGTGGAT TAATCCCGAT GAGATGGAGC TGGATGAGCT CCTGGAGTCT 
AGTGAGGGAG CTCATCTCAG GAGACGTCGA CGGCACACAG ACACCGTAAG ACCGTACATC 
GCCGCAAAGC TATCCACGCT CCCAGAAACC TTCACTCTGG GCGATGAGCT AGACTACAGC 
GGCTTCATCA ACCGGCCTCT TCCCAACAAC CAACACTTCC AGTGTTTTGT GATGGCTGAA 
CTCAAGGACC AATACCCTGT TACTGCCAAT GAGAAGCAGC GGACCTTCTC GGCTAGCCCA 
TACTCAGACC CCATCATAGT GCAGGGTGAA AACCAAATGC AGCGCAGTGT GGACCAACCG 
GAGATGCTTT GGGTCATGGG CCCTGTGCTC GCTGTGGTGC TCATCATCAT CATAGTCATC 
GCCATCCTGC TCTTCAAAAG CAAACAGGAG AGGAAGAGGG CTTCCCCCCT ACCAAAAGAC 
GATCATTCGG GTGGTGTGAA GGATTGTCTT CTGGCCAGCT CATCCGATCC TGTCGAAATG 
AGGAGACTCA ACTACCAGAC ACCAGGAATG AGGGAACATC CTCCCATATC CGTCTCCGAA 
CTGGCAGACC ACATCGAGCG CCTCAAGGCC AACGATGCCC TCCGGTTTTC CCAAGAGTAT 
GAGTCTATTG ATCCAGGCCA GCAGTTCACA TGGGAGAACT CCAACTTGGA GGTCAACAAG 
CCAAAGAACC GTTATGCCAA CGTTATTGCT TACGACCACT CGAGAGTTGT TCTCACCCCT 
GTGGACGGTG TACCAGGGAG TGACTACATT AATTCCAACT ACATTGATGG CTACCGTAAG 
CAGAATGCCT ACATTGCCAC ACAAGGTCCT CTGCCTGAGA CTCTGAGCGA CTTCTGGAGG 
ATGATTTGGG AGCAACGAGC CAGTACCATC GTCATGATGA CCAGACTGGA AGAGAAGTCC 
AGAGTCAAGT GTGACCAATA CTGGCCCATT CGTGGCACAG AGACTTATGG AATGATTCAA 
GTGACCATGC TTGATGCTGT TGAGCTGGCA ACCTACAGTG TGCGCACCTT TGCCCTTTAC 
AAGAATGGCT CTAGTGAGAA GCGCGAGGTC AGGCAGTTTC AGTTCATGGC TTGGCCGGAC 
CACGGAGTGC CTGAATATCC CACCCCCATC CTAGCCTTCC TGCGCAGGGT CAAAGCCTGC 
AACCCTCCTG ATGCTGGACC CATGGTAGTG CACTGCAGTG CTGGAGTTGG CCGTACAGGC 
TGCTTCATAG TCATCGATGC GATGTTGGAG CGCATGAAGC ACGAGAAGAC CGTAGACATT 
TATGGCCACG TCACGTGCAT GAGATCCCAG AGGAACTATA TGGTGCAGAC GGAGGACCAG 
TACATCTTTA TCCACGAGGC CCTTCTGGAG GCCGCCACCT GTGGAAACAC AGAGGTCCCT 
GCCCGAAACC TCTACGCCCA CATCCAGAAG CTCACACAGC CATCGGCTGG AGAGACCGTC 
ACAGCCATGG AGCTGGAGTT TAAGAGACTC GCCAACTCCA AAGCCCACAC CTCAAGATTC 
ATCAGCGCCA ACCTGCCCTG CAACAAGTTC AAGAACCGGC TGGTCAATAT CATGCCTTTC 
GAGTCCACAC GGGTGTGCCT GCAGCCAATC CGAGGAGTGG AGGGGTCCGA CTACATCAAT 
GCCAGCTGCA TTGACGGATA CAGACAGCAG AAGGCCTATA TTGCCACTCA GGGTCCTCTA 
GCGGAGACCA CCGAGGACTT CTGGAGGATG TTATGGGAGC ACAATTCCAC TATTGTAGTG 
ATGCTGACCA AACTCAGAGA GATGGGACGG GAGAAGTGTC ATCAGTACTG GCCCGCCGAG 
CGCTCAGCCC GCTATCAGTA CTTCGTAGTG GATCCCATGG CCGAATACAA CATGCCCCAG 
TACATCCTGC GAGAGTTCAA AGTCACAGAT GCCAGGGACG GTCAATCCAG AACCATTCGC 
CAGTTCCAGT TCACTGATTG GCCAGAGCAG GGAGTGCCAA AAACCGGTGA GGGGTTCATC 
GATTTCATTG GGCAAGTTCA TAAAACCAAG GAGCAGTTTG GGCAGGATGG ACCAATCACT 
GTGCACTGCA GTGCTGGAGT GGGAAGAACA GGTGTTTTCA TCACTCTCAG TATTGTCCTG 
GAGAGGATGA GATATGAGGG GGTGGTGGAT CTGTTCCAGA CGGTTAAAAC CCTGCGCACA 
CAAAGGCCTG CCATGGTGCA GACAGAGGAC CAGTACCAGC TGTGCTACAG AGCCGCTCTG 
GAGTACCTGG GGAGCTTTGA TCACTATGCA ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_021481441.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337116.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X18, mRNA.

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CloneID	ODa150378
Clone ID Related Accession (Same CDS sequence)	XM_021481441.1
Accession Version	XM_021481441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TACGACGAGT TCCACCCCGT 
TTCTCCATCC CACCTACCAA TCACGAGGTG ATGCCAGGGG GCAGTGTAAA CCTCACCTGC 
GTGGCAGTAG GTGCCCCGAT GCCCTACGTC AAATGGATGA CTGGTGAGGT GGAGCTCACC 
AAGGATGAGG AGATGCCTGT GGGACGCAAC GTCTTGGAGC TCACCAACAT CCGTCAGTCG 
ACCAACTACA CCTGCGTGGC TATATCATCC CTAGGTATGA TCGAGGCAAC GGCACAAGTG 
TCTGTCAAAG CTCTCCCAAA GCCTCCTACA TCCCTGACTG TTACGGAAAC CACAGCTACG 
AGTGTTACAC TCACATGGGA CTCTGGAAAC CCTGAACCTG TGTCTTACTA CATAATTCAA 
TACCGGGCCA AGACTTCAGA CACCAACTTC CAAGAAGTGG ACGGTGTGGC CACCACACGT 
TACAGCATCG GCGGTCTCAG CCCATATTCA GAGTATGAGT TTCGAGTAAT GGCAGTCAAC 
AACATTGGTC GTGGGCCACC TAGTGAACCC GTTGAAACAC GAACAGGTGA ACAGGCACCT 
TCCTCCCCAC CGCTGCATGT TCAAGCACGA ATGCTGAGTG CCAGCACCAT GTTGGTTCAG 
TGGGAACCAC CAGAGGAACC CAATGGTCAG ATCAGAGGCT ATCGGATCTA CTACACATCT 
GACCTGGATG CACCTCTTAG CGCCTGGCAG AAACACAACA CAGACGATAG CCGTCTCACC 
ACTATCTCCA GCCTGACCAC CGGCATCACC TACAGCCTAC GTGTGCTGGG CTTCACATCT 
GTAGGGGATG GACCACCATC TGATGTGCTA CAGGTCAAGA CACAGCAAGG AGTTCCTGCT 
CAGCCATCCA GCTTCGAAGC TGAAGCAGAG CTAGATACAA GGATCCTACT AACTTGGCTG 
TGGCCCGTGC AGGACCCCAT CATCAAATAC GAGCTGCAAT ATTGGGAGGC TGACTCAGAC 
AACAAGATCC ATGTCACCTT TGACCCAGCT GGCTCGTACG CTGTTGAAGG TCTGAAACCA 
GACACTTTGT ACAAATTCTC TCTGGCAGCC CGCTCTGAAA TGGGACAAGG AGTCTTCACT 
CACCCCATTG AAGCCAGGAC GGCCCAGTCT GTGCCAGGAG CACCGCCTCG GAAATTGGAA 
GTGGAGGCTT TGAACTCCAC CGCTATCCGT GTGACATGGA AACCTCCTCT TTCTGGAAAG 
CAGCATGGCC AGATCCGGGG ATATCAGGTC ATATACTCCC GGTTGGAGAA CGGGGAACCA 
CGGGGACATC CGAATATAAT GGATGTCGCT CTGCCAGAAG CACAGTGGAA AATTGAAGAG 
TCTACGGAAT ATGAGGCTGT GATCGCTGGA CTTGCCTCTG AAACCTCTTA CTCAGTGACT 
GTGGCTGCGT ACACAACCAA AGGTGATGGA GCTCGCAGCA AAGCCAAAGT GGTCACCACC 
ACCGGAGCAG TGCCTGGCAA ACCCACTATG ATCATCAGCA CCACCGTTGG CAACACGGCC 
CTTATCCAGT GGCAGCCTCC TAAAGACATG GTGGGTGAGC TGATGGGCTA CCGGCTGCGC 
TACAAACGTC TGGAAGAGGA AAACTATGAG ATACGCGAAT TCAGTCGCAC CGACGACCAC 
CACACCGTCA CTGACCTGCA CAAGGGCGCC ACCTACAGCT TCCACCTGAG CGCCAGAAAC 
CGCGCTGGAC TCGGAGAGGA GTACGTTAAA GACATCGGCA CTCCTGAGGA CGTGCCCAGC 
GGGTTTCCTC TTAACCTGCA GGTCGTAGGC TTGACTACCT CTACCACCCG ACTGACCTGG 
GAGCCTCCAG CGTTGTCTGA ACGTAACGGA CGGATCATAC ATTACATTGT GGTTTACCGA 
GACATCAACA GCAAACAAAA CAGCACCAAC CGAACCTCCG ACACACAGAT GACCATCCAA 
GGGCTCCGAC CTGACACCAC CTATGATATC CGTGTGCAGG CGTTCACTAG CAAGGGTGGA 
GGTCCCATAA GTCCCAGTAT CCAGAGCAGG ACCATGTCTA CGTCACCTGC ATTTGCCACA 
AGTTTTGGTG TCAAAGCTGT GATGAAAACC TCTGTCCTTC TCACCTGGGA GGTGCCAGAA 
AATTATAAAT CACAAGTGCC TTTTAAGATC CTCTATAACC AGCAGAGTGT GGAAGTCCAG 
GGGAATCTGA AGAGGAAGCT GATCACACGC CTGCAGCCGG ACACTGATTA CTCGTTTGTG 
CTGATGAGCC GGGGAAACAG CGCCGGTGGC CTACAACAGC AGGTCTCCAT CCGCACTGCA 
CCAGACCTGT TCAAAACTAA ACCTGTCCTG TACACGGCAG ACCAGACCAA CAATGGCAAA 
CTCACCATCA ACCTGCCCAA AGTGCCCACC TCTGCTCCTG TCAGGTGTTA TTACATTGTG 
GTCGTGCCCG TCTCTCAGAC ATCATCTCGT CAGTGGATTA ATCCCGATGA GATGGAGCTG 
GATGAGCTCC TGGAGTCTAG TGAGGGAGCT CATCTCAGGA GACGTCGACG GCACACAGAC 
ACCGTAAGAC CGTACATCGC CGCAAAGCTA TCCACGCTCC CAGAAACCTT CACTCTGGGC 
GATGAGCTAG ACTACAGCGG CTTCATCAAC CGGCCTCTTC CCAACAACCA ACACTTCCAG 
TGTTTTGTGA TGGCTGAACT CAAGGACCAA TACCCTGTTA CTGCCAATGA GAAGCAGCGG 
ACCTTCTCGG CTAGCCCATA CTCAGACCCC ATCATAGTGC AGGGTGAAAA CCAAATGCAG 
CGCAGTGTGG ACCAACCGGA GATGCTTTGG GTCATGGGCC CTGTGCTCGC TGTGGTGCTC 
ATCATCATCA TAGTCATCGC CATCCTGCTC TTCAAAAGGA AGAGGGCTTC CCCCCTACCA 
AAAGACGATC ATTCGGGTGG TGTGAAGGAT TGTCTTCTGG CCAGCTCATC CGATCCTGTC 
GAAATGAGGA GACTCAACTA CCAGACACCA GGAATGAGGG AACATCCTCC CATATCCGTC 
TCCGAACTGG CAGACCACAT CGAGCGCCTC AAGGCCAACG ATGCCCTCCG GTTTTCCCAA 
GAGTATGAGT CTATTGATCC AGGCCAGCAG TTCACATGGG AGAACTCCAA CTTGGAGGTC 
AACAAGCCAA AGAACCGTTA TGCCAACGTT ATTGCTTACG ACCACTCGAG AGTTGTTCTC 
ACCCCTGTGG ACGGTGTACC AGGGAGTGAC TACATTAATT CCAACTACAT TGATGGCTAC 
CGTAAGCAGA ATGCCTACAT TGCCACACAA GGTCCTCTGC CTGAGACTCT GAGCGACTTC 
TGGAGGATGA TTTGGGAGCA ACGAGCCAGT ACCATCGTCA TGATGACCAG ACTGGAAGAG 
AAGTCCAGAG TCAAGTGTGA CCAATACTGG CCCATTCGTG GCACAGAGAC TTATGGAATG 
ATTCAAGTGA CCATGCTTGA TGCTGTTGAG CTGGCAACCT ACAGTGTGCG CACCTTTGCC 
CTTTACAAGA ATGGCTCTAG TGAGAAGCGC GAGGTCAGGC AGTTTCAGTT CATGGCTTGG 
CCGGACCACG GAGTGCCTGA ATATCCCACC CCCATCCTAG CCTTCCTGCG CAGGGTCAAA 
GCCTGCAACC CTCCTGATGC TGGACCCATG GTAGTGCACT GCAGTGCTGG AGTTGGCCGT 
ACAGGCTGCT TCATAGTCAT CGATGCGATG TTGGAGCGCA TGAAGCACGA GAAGACCGTA 
GACATTTATG GCCACGTCAC GTGCATGAGA TCCCAGAGGA ACTATATGGT GCAGACGGAG 
GACCAGTACA TCTTTATCCA CGAGGCCCTT CTGGAGGCCG CCACCTGTGG AAACACAGAG 
GTCCCTGCCC GAAACCTCTA CGCCCACATC CAGAAGCTCA CACAGCCATC GGCTGGAGAG 
ACCGTCACAG CCATGGAGCT GGAGTTTAAG AGACTCGCCA ACTCCAAAGC CCACACCTCA 
AGATTCATCA GCGCCAACCT GCCCTGCAAC AAGTTCAAGA ACCGGCTGGT CAATATCATG 
CCTTTCGAGT CCACACGGGT GTGCCTGCAG CCAATCCGAG GAGTGGAGGG GTCCGACTAC 
ATCAATGCCA GCTGCATTGA CGGATACAGA CAGCAGAAGG CCTATATTGC CACTCAGGGT 
CCTCTAGCGG AGACCACCGA GGACTTCTGG AGGATGTTAT GGGAGCACAA TTCCACTATT 
GTAGTGATGC TGACCAAACT CAGAGAGATG GGACGGGAGA AGTGTCATCA GTACTGGCCC 
GCCGAGCGCT CAGCCCGCTA TCAGTACTTC GTAGTGGATC CCATGGCCGA ATACAACATG 
CCCCAGTACA TCCTGCGAGA GTTCAAAGTC ACAGATGCCA GGGACGGTCA ATCCAGAACC 
ATTCGCCAGT TCCAGTTCAC TGATTGGCCA GAGCAGGGAG TGCCAAAAAC CGGTGAGGGG 
TTCATCGATT TCATTGGGCA AGTTCATAAA ACCAAGGAGC AGTTTGGGCA GGATGGACCA 
ATCACTGTGC ACTGCAGTGC TGGAGTGGGA AGAACAGGTG TTTTCATCAC TCTCAGTATT 
GTCCTGGAGA GGATGAGATA TGAGGGGGTG GTGGATCTGT TCCAGACGGT TAAAACCCTG 
CGCACACAAA GGCCTGCCAT GGTGCAGACA GAGGACCAGT ACCAGCTGTG CTACAGAGCC 
GCTCTGGAGT ACCTGGGGAG CTTTGATCAC TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337116.1
CDS	421..5862
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKRKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021481441.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481441.1

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TACGACGAGT TCCACCCCGT 
TTCTCCATCC CACCTACCAA TCACGAGGTG ATGCCAGGGG GCAGTGTAAA CCTCACCTGC 
GTGGCAGTAG GTGCCCCGAT GCCCTACGTC AAATGGATGA CTGGTGAGGT GGAGCTCACC 
AAGGATGAGG AGATGCCTGT GGGACGCAAC GTCTTGGAGC TCACCAACAT CCGTCAGTCG 
ACCAACTACA CCTGCGTGGC TATATCATCC CTAGGTATGA TCGAGGCAAC GGCACAAGTG 
TCTGTCAAAG CTCTCCCAAA GCCTCCTACA TCCCTGACTG TTACGGAAAC CACAGCTACG 
AGTGTTACAC TCACATGGGA CTCTGGAAAC CCTGAACCTG TGTCTTACTA CATAATTCAA 
TACCGGGCCA AGACTTCAGA CACCAACTTC CAAGAAGTGG ACGGTGTGGC CACCACACGT 
TACAGCATCG GCGGTCTCAG CCCATATTCA GAGTATGAGT TTCGAGTAAT GGCAGTCAAC 
AACATTGGTC GTGGGCCACC TAGTGAACCC GTTGAAACAC GAACAGGTGA ACAGGCACCT 
TCCTCCCCAC CGCTGCATGT TCAAGCACGA ATGCTGAGTG CCAGCACCAT GTTGGTTCAG 
TGGGAACCAC CAGAGGAACC CAATGGTCAG ATCAGAGGCT ATCGGATCTA CTACACATCT 
GACCTGGATG CACCTCTTAG CGCCTGGCAG AAACACAACA CAGACGATAG CCGTCTCACC 
ACTATCTCCA GCCTGACCAC CGGCATCACC TACAGCCTAC GTGTGCTGGG CTTCACATCT 
GTAGGGGATG GACCACCATC TGATGTGCTA CAGGTCAAGA CACAGCAAGG AGTTCCTGCT 
CAGCCATCCA GCTTCGAAGC TGAAGCAGAG CTAGATACAA GGATCCTACT AACTTGGCTG 
TGGCCCGTGC AGGACCCCAT CATCAAATAC GAGCTGCAAT ATTGGGAGGC TGACTCAGAC 
AACAAGATCC ATGTCACCTT TGACCCAGCT GGCTCGTACG CTGTTGAAGG TCTGAAACCA 
GACACTTTGT ACAAATTCTC TCTGGCAGCC CGCTCTGAAA TGGGACAAGG AGTCTTCACT 
CACCCCATTG AAGCCAGGAC GGCCCAGTCT GTGCCAGGAG CACCGCCTCG GAAATTGGAA 
GTGGAGGCTT TGAACTCCAC CGCTATCCGT GTGACATGGA AACCTCCTCT TTCTGGAAAG 
CAGCATGGCC AGATCCGGGG ATATCAGGTC ATATACTCCC GGTTGGAGAA CGGGGAACCA 
CGGGGACATC CGAATATAAT GGATGTCGCT CTGCCAGAAG CACAGTGGAA AATTGAAGAG 
TCTACGGAAT ATGAGGCTGT GATCGCTGGA CTTGCCTCTG AAACCTCTTA CTCAGTGACT 
GTGGCTGCGT ACACAACCAA AGGTGATGGA GCTCGCAGCA AAGCCAAAGT GGTCACCACC 
ACCGGAGCAG TGCCTGGCAA ACCCACTATG ATCATCAGCA CCACCGTTGG CAACACGGCC 
CTTATCCAGT GGCAGCCTCC TAAAGACATG GTGGGTGAGC TGATGGGCTA CCGGCTGCGC 
TACAAACGTC TGGAAGAGGA AAACTATGAG ATACGCGAAT TCAGTCGCAC CGACGACCAC 
CACACCGTCA CTGACCTGCA CAAGGGCGCC ACCTACAGCT TCCACCTGAG CGCCAGAAAC 
CGCGCTGGAC TCGGAGAGGA GTACGTTAAA GACATCGGCA CTCCTGAGGA CGTGCCCAGC 
GGGTTTCCTC TTAACCTGCA GGTCGTAGGC TTGACTACCT CTACCACCCG ACTGACCTGG 
GAGCCTCCAG CGTTGTCTGA ACGTAACGGA CGGATCATAC ATTACATTGT GGTTTACCGA 
GACATCAACA GCAAACAAAA CAGCACCAAC CGAACCTCCG ACACACAGAT GACCATCCAA 
GGGCTCCGAC CTGACACCAC CTATGATATC CGTGTGCAGG CGTTCACTAG CAAGGGTGGA 
GGTCCCATAA GTCCCAGTAT CCAGAGCAGG ACCATGTCTA CGTCACCTGC ATTTGCCACA 
AGTTTTGGTG TCAAAGCTGT GATGAAAACC TCTGTCCTTC TCACCTGGGA GGTGCCAGAA 
AATTATAAAT CACAAGTGCC TTTTAAGATC CTCTATAACC AGCAGAGTGT GGAAGTCCAG 
GGGAATCTGA AGAGGAAGCT GATCACACGC CTGCAGCCGG ACACTGATTA CTCGTTTGTG 
CTGATGAGCC GGGGAAACAG CGCCGGTGGC CTACAACAGC AGGTCTCCAT CCGCACTGCA 
CCAGACCTGT TCAAAACTAA ACCTGTCCTG TACACGGCAG ACCAGACCAA CAATGGCAAA 
CTCACCATCA ACCTGCCCAA AGTGCCCACC TCTGCTCCTG TCAGGTGTTA TTACATTGTG 
GTCGTGCCCG TCTCTCAGAC ATCATCTCGT CAGTGGATTA ATCCCGATGA GATGGAGCTG 
GATGAGCTCC TGGAGTCTAG TGAGGGAGCT CATCTCAGGA GACGTCGACG GCACACAGAC 
ACCGTAAGAC CGTACATCGC CGCAAAGCTA TCCACGCTCC CAGAAACCTT CACTCTGGGC 
GATGAGCTAG ACTACAGCGG CTTCATCAAC CGGCCTCTTC CCAACAACCA ACACTTCCAG 
TGTTTTGTGA TGGCTGAACT CAAGGACCAA TACCCTGTTA CTGCCAATGA GAAGCAGCGG 
ACCTTCTCGG CTAGCCCATA CTCAGACCCC ATCATAGTGC AGGGTGAAAA CCAAATGCAG 
CGCAGTGTGG ACCAACCGGA GATGCTTTGG GTCATGGGCC CTGTGCTCGC TGTGGTGCTC 
ATCATCATCA TAGTCATCGC CATCCTGCTC TTCAAAAGGA AGAGGGCTTC CCCCCTACCA 
AAAGACGATC ATTCGGGTGG TGTGAAGGAT TGTCTTCTGG CCAGCTCATC CGATCCTGTC 
GAAATGAGGA GACTCAACTA CCAGACACCA GGAATGAGGG AACATCCTCC CATATCCGTC 
TCCGAACTGG CAGACCACAT CGAGCGCCTC AAGGCCAACG ATGCCCTCCG GTTTTCCCAA 
GAGTATGAGT CTATTGATCC AGGCCAGCAG TTCACATGGG AGAACTCCAA CTTGGAGGTC 
AACAAGCCAA AGAACCGTTA TGCCAACGTT ATTGCTTACG ACCACTCGAG AGTTGTTCTC 
ACCCCTGTGG ACGGTGTACC AGGGAGTGAC TACATTAATT CCAACTACAT TGATGGCTAC 
CGTAAGCAGA ATGCCTACAT TGCCACACAA GGTCCTCTGC CTGAGACTCT GAGCGACTTC 
TGGAGGATGA TTTGGGAGCA ACGAGCCAGT ACCATCGTCA TGATGACCAG ACTGGAAGAG 
AAGTCCAGAG TCAAGTGTGA CCAATACTGG CCCATTCGTG GCACAGAGAC TTATGGAATG 
ATTCAAGTGA CCATGCTTGA TGCTGTTGAG CTGGCAACCT ACAGTGTGCG CACCTTTGCC 
CTTTACAAGA ATGGCTCTAG TGAGAAGCGC GAGGTCAGGC AGTTTCAGTT CATGGCTTGG 
CCGGACCACG GAGTGCCTGA ATATCCCACC CCCATCCTAG CCTTCCTGCG CAGGGTCAAA 
GCCTGCAACC CTCCTGATGC TGGACCCATG GTAGTGCACT GCAGTGCTGG AGTTGGCCGT 
ACAGGCTGCT TCATAGTCAT CGATGCGATG TTGGAGCGCA TGAAGCACGA GAAGACCGTA 
GACATTTATG GCCACGTCAC GTGCATGAGA TCCCAGAGGA ACTATATGGT GCAGACGGAG 
GACCAGTACA TCTTTATCCA CGAGGCCCTT CTGGAGGCCG CCACCTGTGG AAACACAGAG 
GTCCCTGCCC GAAACCTCTA CGCCCACATC CAGAAGCTCA CACAGCCATC GGCTGGAGAG 
ACCGTCACAG CCATGGAGCT GGAGTTTAAG AGACTCGCCA ACTCCAAAGC CCACACCTCA 
AGATTCATCA GCGCCAACCT GCCCTGCAAC AAGTTCAAGA ACCGGCTGGT CAATATCATG 
CCTTTCGAGT CCACACGGGT GTGCCTGCAG CCAATCCGAG GAGTGGAGGG GTCCGACTAC 
ATCAATGCCA GCTGCATTGA CGGATACAGA CAGCAGAAGG CCTATATTGC CACTCAGGGT 
CCTCTAGCGG AGACCACCGA GGACTTCTGG AGGATGTTAT GGGAGCACAA TTCCACTATT 
GTAGTGATGC TGACCAAACT CAGAGAGATG GGACGGGAGA AGTGTCATCA GTACTGGCCC 
GCCGAGCGCT CAGCCCGCTA TCAGTACTTC GTAGTGGATC CCATGGCCGA ATACAACATG 
CCCCAGTACA TCCTGCGAGA GTTCAAAGTC ACAGATGCCA GGGACGGTCA ATCCAGAACC 
ATTCGCCAGT TCCAGTTCAC TGATTGGCCA GAGCAGGGAG TGCCAAAAAC CGGTGAGGGG 
TTCATCGATT TCATTGGGCA AGTTCATAAA ACCAAGGAGC AGTTTGGGCA GGATGGACCA 
ATCACTGTGC ACTGCAGTGC TGGAGTGGGA AGAACAGGTG TTTTCATCAC TCTCAGTATT 
GTCCTGGAGA GGATGAGATA TGAGGGGGTG GTGGATCTGT TCCAGACGGT TAAAACCCTG 
CGCACACAAA GGCCTGCCAT GGTGCAGACA GAGGACCAGT ACCAGCTGTG CTACAGAGCC 
GCTCTGGAGT ACCTGGGGAG CTTTGATCAC TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_021481443.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337118.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X19, mRNA.

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CloneID	ODa150379
Clone ID Related Accession (Same CDS sequence)	XM_021481443.1
Accession Version	XM_021481443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGTGCCAGG AGCACCGCCT CGGAAATTGG AAGTGGAGGC TTTGAACTCC 
ACCGCTATCC GTGTGACATG GAAACCTCCT CTTTCTGGAA AGCAGCATGG CCAGATCCGG 
GGATATCAGG TCATATACTC CCGGTTGGAG AACGGGGAAC CACGGGGACA TCCGAATATA 
ATGGATGTCG CTCTGCCAGA AGCACAGTGG AAAATTGAAG AGTCTACGGA ATATGAGGCT 
GTGATCGCTG GACTTGCCTC TGAAACCTCT TACTCAGTGA CTGTGGCTGC GTACACAACC 
AAAGGTGATG GAGCTCGCAG CAAAGCCAAA GTGGTCACCA CCACCGGAGC AGTGCCTGGC 
AAACCCACTA TGATCATCAG CACCACCGTT GGCAACACGG CCCTTATCCA GTGGCAGCCT 
CCTAAAGACA TGGTGGGTGA GCTGATGGGC TACCGGCTGC GCTACAAACG TCTGGAAGAG 
GAAAACTATG AGATACGCGA ATTCAGTCGC ACCGACGACC ACCACACCGT CACTGACCTG 
CACAAGGGCG CCACCTACAG CTTCCACCTG AGCGCCAGAA ACCGCGCTGG ACTCGGAGAG 
GAGTACGTTA AAGACATCGG CACTCCTGAG GACGTGCCCA GCGGGTTTCC TCTTAACCTG 
CAGGTCGTAG GCTTGACTAC CTCTACCACC CGACTGACCT GGGAGCCTCC AGCGTTGTCT 
GAACGTAACG GACGGATCAT ACATTACATT GTGGTTTACC GAGACATCAA CAGCAAACAA 
AACAGCACCA ACCGAACCTC CGACACACAG ATGACCATCC AAGGGCTCCG ACCTGACACC 
ACCTATGATA TCCGTGTGCA GGCGTTCACT AGCAAGGGTG GAGGTCCCAT AAGTCCCAGT 
ATCCAGAGCA GGACCATGTC TACGTCACCT GCATTTGCCA CAAGTTTTGG TGTCAAAGCT 
GTGATGAAAA CCTCTGTCCT TCTCACCTGG GAGGTGCCAG AAAATTATAA ATCACAAGTG 
CCTTTTAAGA TCCTCTATAA CCAGCAGAGT GTGGAAGTCC AGGGGAATCT GAAGAGGAAG 
CTGATCACAC GCCTGCAGCC GGACACTGAT TACTCGTTTG TGCTGATGAG CCGGGGAAAC 
AGCGCCGGTG GCCTACAACA GCAGGTCTCC ATCCGCACTG CACCAGACCT GTTCAAAACT 
AAACCTGTCC TGTACACGGC AGACCAGACC AACAATGGCA AACTCACCAT CAACCTGCCC 
AAAGTGCCCA CCTCTGCTCC TGTCAGGTGT TATTACATTG TGGTCGTGCC CGTCTCTCAG 
ACATCATCTC GTCAGTGGAT TAATCCCGAT GAGATGGAGC TGGATGAGCT CCTGGAGTCT 
AGTGAGGGAG CTCATCTCAG GAGACGTCGA CGGCACACAG ACACCGTAAG ACCGTACATC 
GCCGCAAAGC TATCCACGCT CCCAGAAACC TTCACTCTGG GCGATGAGCT AGACTACAGC 
GGCTTCATCA ACCGGCCTCT TCCCAACAAC CAACACTTCC AGTGTTTTGT GATGGCTGAA 
CTCAAGGACC AATACCCTGT TACTGCCAAT GAGAAGCAGC GGACCTTCTC GGCTAGCCCA 
TACTCAGACC CCATCATAGT GCAGGGTGAA AACCAAATGC AGCGCAGTGT GGACCAACCG 
GAGATGCTTT GGGTCATGGG CCCTGTGCTC GCTGTGGTGC TCATCATCAT CATAGTCATC 
GCCATCCTGC TCTTCAAAAG GAAGAGGGCT TCCCCCCTAC CAAAAGACGA TCATTCGGGT 
GGTGTGAAGG ATTGTCTTCT GGCCAGCTCA TCCGATCCTG TCGAAATGAG GAGACTCAAC 
TACCAGACAC CAGGAATGAG GGAACATCCT CCCATATCCG TCTCCGAACT GGCAGACCAC 
ATCGAGCGCC TCAAGGCCAA CGATGCCCTC CGGTTTTCCC AAGAGTATGA GTCTATTGAT 
CCAGGCCAGC AGTTCACATG GGAGAACTCC AACTTGGAGG TCAACAAGCC AAAGAACCGT 
TATGCCAACG TTATTGCTTA CGACCACTCG AGAGTTGTTC TCACCCCTGT GGACGGTGTA 
CCAGGGAGTG ACTACATTAA TTCCAACTAC ATTGATGGCT ACCGTAAGCA GAATGCCTAC 
ATTGCCACAC AAGGTCCTCT GCCTGAGACT CTGAGCGACT TCTGGAGGAT GATTTGGGAG 
CAACGAGCCA GTACCATCGT CATGATGACC AGACTGGAAG AGAAGTCCAG AGTCAAGTGT 
GACCAATACT GGCCCATTCG TGGCACAGAG ACTTATGGAA TGATTCAAGT GACCATGCTT 
GATGCTGTTG AGCTGGCAAC CTACAGTGTG CGCACCTTTG CCCTTTACAA GAATGGCTCT 
AGTGAGAAGC GCGAGGTCAG GCAGTTTCAG TTCATGGCTT GGCCGGACCA CGGAGTGCCT 
GAATATCCCA CCCCCATCCT AGCCTTCCTG CGCAGGGTCA AAGCCTGCAA CCCTCCTGAT 
GCTGGACCCA TGGTAGTGCA CTGCAGTGCT GGAGTTGGCC GTACAGGCTG CTTCATAGTC 
ATCGATGCGA TGTTGGAGCG CATGAAGCAC GAGAAGACCG TAGACATTTA TGGCCACGTC 
ACGTGCATGA GATCCCAGAG GAACTATATG GTGCAGACGG AGGACCAGTA CATCTTTATC 
CACGAGGCCC TTCTGGAGGC CGCCACCTGT GGAAACACAG AGGTCCCTGC CCGAAACCTC 
TACGCCCACA TCCAGAAGCT CACACAGCCA TCGGCTGGAG AGACCGTCAC AGCCATGGAG 
CTGGAGTTTA AGAGACTCGC CAACTCCAAA GCCCACACCT CAAGATTCAT CAGCGCCAAC 
CTGCCCTGCA ACAAGTTCAA GAACCGGCTG GTCAATATCA TGCCTTTCGA GTCCACACGG 
GTGTGCCTGC AGCCAATCCG AGGAGTGGAG GGGTCCGACT ACATCAATGC CAGCTGCATT 
GACGGATACA GACAGCAGAA GGCCTATATT GCCACTCAGG GTCCTCTAGC GGAGACCACC 
GAGGACTTCT GGAGGATGTT ATGGGAGCAC AATTCCACTA TTGTAGTGAT GCTGACCAAA 
CTCAGAGAGA TGGGACGGGA GAAGTGTCAT CAGTACTGGC CCGCCGAGCG CTCAGCCCGC 
TATCAGTACT TCGTAGTGGA TCCCATGGCC GAATACAACA TGCCCCAGTA CATCCTGCGA 
GAGTTCAAAG TCACAGATGC CAGGGACGGT CAATCCAGAA CCATTCGCCA GTTCCAGTTC 
ACTGATTGGC CAGAGCAGGG AGTGCCAAAA ACCGGTGAGG GGTTCATCGA TTTCATTGGG 
CAAGTTCATA AAACCAAGGA GCAGTTTGGG CAGGATGGAC CAATCACTGT GCACTGCAGT 
GCTGGAGTGG GAAGAACAGG TGTTTTCATC ACTCTCAGTA TTGTCCTGGA GAGGATGAGA 
TATGAGGGGG TGGTGGATCT GTTCCAGACG GTTAAAACCC TGCGCACACA AAGGCCTGCC 
ATGGTGCAGA CAGAGGACCA GTACCAGCTG TGCTACAGAG CCGCTCTGGA GTACCTGGGG 
AGCTTTGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337118.1
CDS	421..5844
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKRKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021481443.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481443.1

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGTGCCAGG AGCACCGCCT CGGAAATTGG AAGTGGAGGC TTTGAACTCC 
ACCGCTATCC GTGTGACATG GAAACCTCCT CTTTCTGGAA AGCAGCATGG CCAGATCCGG 
GGATATCAGG TCATATACTC CCGGTTGGAG AACGGGGAAC CACGGGGACA TCCGAATATA 
ATGGATGTCG CTCTGCCAGA AGCACAGTGG AAAATTGAAG AGTCTACGGA ATATGAGGCT 
GTGATCGCTG GACTTGCCTC TGAAACCTCT TACTCAGTGA CTGTGGCTGC GTACACAACC 
AAAGGTGATG GAGCTCGCAG CAAAGCCAAA GTGGTCACCA CCACCGGAGC AGTGCCTGGC 
AAACCCACTA TGATCATCAG CACCACCGTT GGCAACACGG CCCTTATCCA GTGGCAGCCT 
CCTAAAGACA TGGTGGGTGA GCTGATGGGC TACCGGCTGC GCTACAAACG TCTGGAAGAG 
GAAAACTATG AGATACGCGA ATTCAGTCGC ACCGACGACC ACCACACCGT CACTGACCTG 
CACAAGGGCG CCACCTACAG CTTCCACCTG AGCGCCAGAA ACCGCGCTGG ACTCGGAGAG 
GAGTACGTTA AAGACATCGG CACTCCTGAG GACGTGCCCA GCGGGTTTCC TCTTAACCTG 
CAGGTCGTAG GCTTGACTAC CTCTACCACC CGACTGACCT GGGAGCCTCC AGCGTTGTCT 
GAACGTAACG GACGGATCAT ACATTACATT GTGGTTTACC GAGACATCAA CAGCAAACAA 
AACAGCACCA ACCGAACCTC CGACACACAG ATGACCATCC AAGGGCTCCG ACCTGACACC 
ACCTATGATA TCCGTGTGCA GGCGTTCACT AGCAAGGGTG GAGGTCCCAT AAGTCCCAGT 
ATCCAGAGCA GGACCATGTC TACGTCACCT GCATTTGCCA CAAGTTTTGG TGTCAAAGCT 
GTGATGAAAA CCTCTGTCCT TCTCACCTGG GAGGTGCCAG AAAATTATAA ATCACAAGTG 
CCTTTTAAGA TCCTCTATAA CCAGCAGAGT GTGGAAGTCC AGGGGAATCT GAAGAGGAAG 
CTGATCACAC GCCTGCAGCC GGACACTGAT TACTCGTTTG TGCTGATGAG CCGGGGAAAC 
AGCGCCGGTG GCCTACAACA GCAGGTCTCC ATCCGCACTG CACCAGACCT GTTCAAAACT 
AAACCTGTCC TGTACACGGC AGACCAGACC AACAATGGCA AACTCACCAT CAACCTGCCC 
AAAGTGCCCA CCTCTGCTCC TGTCAGGTGT TATTACATTG TGGTCGTGCC CGTCTCTCAG 
ACATCATCTC GTCAGTGGAT TAATCCCGAT GAGATGGAGC TGGATGAGCT CCTGGAGTCT 
AGTGAGGGAG CTCATCTCAG GAGACGTCGA CGGCACACAG ACACCGTAAG ACCGTACATC 
GCCGCAAAGC TATCCACGCT CCCAGAAACC TTCACTCTGG GCGATGAGCT AGACTACAGC 
GGCTTCATCA ACCGGCCTCT TCCCAACAAC CAACACTTCC AGTGTTTTGT GATGGCTGAA 
CTCAAGGACC AATACCCTGT TACTGCCAAT GAGAAGCAGC GGACCTTCTC GGCTAGCCCA 
TACTCAGACC CCATCATAGT GCAGGGTGAA AACCAAATGC AGCGCAGTGT GGACCAACCG 
GAGATGCTTT GGGTCATGGG CCCTGTGCTC GCTGTGGTGC TCATCATCAT CATAGTCATC 
GCCATCCTGC TCTTCAAAAG GAAGAGGGCT TCCCCCCTAC CAAAAGACGA TCATTCGGGT 
GGTGTGAAGG ATTGTCTTCT GGCCAGCTCA TCCGATCCTG TCGAAATGAG GAGACTCAAC 
TACCAGACAC CAGGAATGAG GGAACATCCT CCCATATCCG TCTCCGAACT GGCAGACCAC 
ATCGAGCGCC TCAAGGCCAA CGATGCCCTC CGGTTTTCCC AAGAGTATGA GTCTATTGAT 
CCAGGCCAGC AGTTCACATG GGAGAACTCC AACTTGGAGG TCAACAAGCC AAAGAACCGT 
TATGCCAACG TTATTGCTTA CGACCACTCG AGAGTTGTTC TCACCCCTGT GGACGGTGTA 
CCAGGGAGTG ACTACATTAA TTCCAACTAC ATTGATGGCT ACCGTAAGCA GAATGCCTAC 
ATTGCCACAC AAGGTCCTCT GCCTGAGACT CTGAGCGACT TCTGGAGGAT GATTTGGGAG 
CAACGAGCCA GTACCATCGT CATGATGACC AGACTGGAAG AGAAGTCCAG AGTCAAGTGT 
GACCAATACT GGCCCATTCG TGGCACAGAG ACTTATGGAA TGATTCAAGT GACCATGCTT 
GATGCTGTTG AGCTGGCAAC CTACAGTGTG CGCACCTTTG CCCTTTACAA GAATGGCTCT 
AGTGAGAAGC GCGAGGTCAG GCAGTTTCAG TTCATGGCTT GGCCGGACCA CGGAGTGCCT 
GAATATCCCA CCCCCATCCT AGCCTTCCTG CGCAGGGTCA AAGCCTGCAA CCCTCCTGAT 
GCTGGACCCA TGGTAGTGCA CTGCAGTGCT GGAGTTGGCC GTACAGGCTG CTTCATAGTC 
ATCGATGCGA TGTTGGAGCG CATGAAGCAC GAGAAGACCG TAGACATTTA TGGCCACGTC 
ACGTGCATGA GATCCCAGAG GAACTATATG GTGCAGACGG AGGACCAGTA CATCTTTATC 
CACGAGGCCC TTCTGGAGGC CGCCACCTGT GGAAACACAG AGGTCCCTGC CCGAAACCTC 
TACGCCCACA TCCAGAAGCT CACACAGCCA TCGGCTGGAG AGACCGTCAC AGCCATGGAG 
CTGGAGTTTA AGAGACTCGC CAACTCCAAA GCCCACACCT CAAGATTCAT CAGCGCCAAC 
CTGCCCTGCA ACAAGTTCAA GAACCGGCTG GTCAATATCA TGCCTTTCGA GTCCACACGG 
GTGTGCCTGC AGCCAATCCG AGGAGTGGAG GGGTCCGACT ACATCAATGC CAGCTGCATT 
GACGGATACA GACAGCAGAA GGCCTATATT GCCACTCAGG GTCCTCTAGC GGAGACCACC 
GAGGACTTCT GGAGGATGTT ATGGGAGCAC AATTCCACTA TTGTAGTGAT GCTGACCAAA 
CTCAGAGAGA TGGGACGGGA GAAGTGTCAT CAGTACTGGC CCGCCGAGCG CTCAGCCCGC 
TATCAGTACT TCGTAGTGGA TCCCATGGCC GAATACAACA TGCCCCAGTA CATCCTGCGA 
GAGTTCAAAG TCACAGATGC CAGGGACGGT CAATCCAGAA CCATTCGCCA GTTCCAGTTC 
ACTGATTGGC CAGAGCAGGG AGTGCCAAAA ACCGGTGAGG GGTTCATCGA TTTCATTGGG 
CAAGTTCATA AAACCAAGGA GCAGTTTGGG CAGGATGGAC CAATCACTGT GCACTGCAGT 
GCTGGAGTGG GAAGAACAGG TGTTTTCATC ACTCTCAGTA TTGTCCTGGA GAGGATGAGA 
TATGAGGGGG TGGTGGATCT GTTCCAGACG GTTAAAACCC TGCGCACACA AAGGCCTGCC 
ATGGTGCAGA CAGAGGACCA GTACCAGCTG TGCTACAGAG CCGCTCTGGA GTACCTGGGG 
AGCTTTGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( NM_001083576.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001077045.1 Danio rerio protein tyrosine phosphatase receptor type Fb (ptprfb), mRNA.

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CloneID	ODa00569
Clone ID Related Accession (Same CDS sequence)	NM_001083576.1
Accession Version	NM_001083576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134804.1. On Mar 28, 2007 this sequence version replaced XM_001334493.1. ##Evidence-Data-START## Transcript exon combination :: BC134804.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT CAAGACGTGC ACCTGCTCAG TCTGAGCTCC 
ACCAGCATCA AGGTGAGTTG GGTGGCACCA CCCGCCGCTA GCCGCCACGG CGCAATAGTG 
CGCTACACAG TCAGCTACCA GGCAGTGAAT GGAGAAGACA CAGAGCGCCA TGAGGTGCCG 
GACATTGGAG CGGATGCCTC CAGCTGTGTG TTGGAAGGTC TAGAGAAGTG GACGGAGTAT 
CAGGTGTGGG TGAGAGCGCA CACTGATGTG GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT 
ATCCGAACCA ATGAAGACGT GCCAGGAGCA CCGCCTCGGA AATTGGAAGT GGAGGCTTTG 
AACTCCACCG CTATCCGTGT GACATGGAAA CCTCCTCTTT CTGGAAAGCA GCATGGCCAG 
ATCCGGGGAT ATCAGGTCAT ATACTCCCGG TTGGAGAACG GGGAACCACG GGGACATCCG 
AATATAATGG ATGTCGCTCT GCCAGAAGCA CAGTGGAAAA TTGAAGAGTC TACGGAATAT 
GAGGCTGTGA TCGCTGGACT TGCCTCTGAA ACCTCTTACT CAGTGACTGT GGCTGCGTAC 
ACAACCAAAG GTGATGGAGC TCGCAGCAAA GCCAAAGTGG TCACCACCAC CGGAGCAGTG 
CCTGGCAAAC CCACTATGAT CATCAGCACC ACCGTTGGCA ACACGGCCCT TATCCAGTGG 
CAGCCTCCTA AAGACATGGT GGGTGAGCTG ATGGGCTACC GGCTGCGCTA CAAACGTCTG 
GAAGAGGAAA ACTATGAGAT ACGCGAATTC AGTCGCACCG ACGACCACCA CACCGTCACT 
GACCTGCACA AGGGCGCCAC CTACAGCTTC CACCTGAGCG CCAGAAACCG CGCTGGACTC 
GGAGAGGAGT ACGTTAAAGA CATCGGCACT CCTGAGGACG TGCCCAGCGG GTTTCCTCTT 
AACCTGCAGG TCGTAGGCTT GACTACCTCT ACCACCCGAC TGACCTGGGA GCCTCCAGCG 
TTGTCTGAAC GTAACGGACG GATCATACAT TACATTGTGG TTTACCGAGA CATCAACAGC 
AAACAAAACA GCACCAACCG AACCTCCGAC ACACAGATGA CCATCCAAGG GCTCCGACCT 
GACACCACCT ATGATATCCG TGTGCAGGCG TTCACTAGCA AGGGTGGAGG TCCCATAAGT 
CCCAGTATCC AGAGCAGGAC CATGTCTACG TCACCTGCAT TTGCCACAAG TTTTGGTGTC 
AAAGCTGTGA TGAAAACCTC TGTCCTTCTC ACCTGGGAGG TGCCAGAAAA TTATAAATCA 
CAAGTGCCTT TTAAGATCCT CTATAACCAG CAGAGTGTGG AAGTCCAGGG GAATCTGAAG 
AGGAAGCTGA TCACACGCCT GCAGCCGGAC ACTGATTACT CGTTTGTGCT GATGAGCCGG 
GGAAACAGCG CCGGTGGCCT ACAACAGCAG GTCTCCATCC GCACTGCACC AGACCTGTTC 
AAAACTAAAC CTGTCCTGTA CACGGCAGAC CAGACCAACA ATGGCAAACT CACCATCAAC 
CTGCCCAAAG TGCCCACCTC TGCTCCTGTC AGGTGTTATT ACATTGTGGT CGTGCCCGTC 
TCTCAGACAT CATCTCGTCA GTGGATTAAT CCCGATGAGA TGGAGCTGGA TGAGCTCCTG 
GAGTCTAGTG AGGGAGCTCA TCTCAGGAGA CGTCGACGGC ACACAGACAC CGTAAGACCG 
TACATCGCCG CAAAGCTATC CACGCTCCCA GAAACCTTCA CTCTGGGCGA TGAGCTAGAC 
TACAGCGGCT TCATCAACCG GCCTCTTCCC AACAACCAAC ACTTCCAGTG TTTTGTGATG 
GCTGAACTCA AGGACCAATA CCCTGTTACT GCCAATGAGA AGCAGCGGAC CTTCTCGGCT 
AGCCCATACT CAGACCCCAT CATAGTGCAG GGTGAAAACC AAATGCAGCG CAGTGTGGAC 
CAACCGGAGA TGCTTTGGGT CATGGGCCCT GTGCTCGCTG TGGTGCTCAT CATCATCATA 
GTCATCGCCA TCCTGCTCTT CAAAAGGAAG AGGGCTTCCC CCCTACCAAA AGACGATCAT 
TCGGGTGGTG TGAAGGATTG TCTTCTGGCC AGCTCATCCG ATCCTGTCGA AATGAGGAGA 
CTCAACTACC AGACACCAGG AATGAGGGAA CATCCTCCCA TATCCGTCTC CGAACTGGCA 
GACCACATCG AGCGCCTCAA GGCCAACGAT GCCCTCCGGT TTTCCCAAGA GTATGAGTCT 
ATTGATCCAG GCCAGCAGTT CACATGGGAG AACTCCAACT TGGAGGTCAA CAAGCCAAAG 
AACCGTTATG CCAACGTTAT TGCTTACGAC CACTCGAGAG TTGTTCTCAC CCCTGTGGAC 
GGTGTACCAG GGAGTGACTA CATTAATTCC AACTACATTG ATGGCTACCG TAAGCAGAAT 
GCCTACATTG CCACACAAGG TCCTCTGCCT GAGACTCTGA GCGACTTCTG GAGGATGATT 
TGGGAGCAAC GAGCCAGTAC CATCGTCATG ATGACCAGAC TGGAAGAGAA GTCCAGAGTC 
AAGTGTGACC AATACTGGCC CATTCGTGGC ACAGAGACTT ATGGAATGAT TCAAGTGACC 
ATGCTTGATG CTGTTGAGCT GGCAACCTAC AGTGTGCGCA CCTTTGCCCT TTACAAGAAT 
GGCTCTAGTG AGAAGCGCGA GGTCAGGCAG TTTCAGTTCA TGGCTTGGCC GGACCACGGA 
GTGCCTGAAT ATCCCACCCC CATCCTAGCC TTCCTGCGCA GGGTCAAAGC CTGCAACCCT 
CCTGATGCTG GACCCATGGT AGTGCACTGC AGTGCTGGAG TTGGCCGTAC AGGCTGCTTC 
ATAGTCATCG ATGCGATGTT GGAGCGCATG AAGCACGAGA AGACCGTAGA CATTTATGGC 
CACGTCACGT GCATGAGATC CCAGAGGAAC TATATGGTGC AGACGGAGGA CCAGTACATC 
TTTATCCACG AGGCCCTTCT GGAGGCCGCC ACCTGTGGAA ACACAGAGGT CCCTGCCCGA 
AACCTCTACG CCCACATCCA GAAGCTCACA CAGCCATCGG CTGGAGAGAC CGTCACAGCC 
ATGGAGCTGG AGTTTAAGAG ACTCGCCAAC TCCAAAGCCC ACACCTCAAG ATTCATCAGC 
GCCAACCTGC CCTGCAACAA GTTCAAGAAC CGGCTGGTCA ATATCATGCC TTTCGAGTCC 
ACACGGGTGT GCCTGCAGCC AATCCGAGGA GTGGAGGGGT CCGACTACAT CAATGCCAGC 
TGCATTGACG GATACAGACA GCAGAAGGCC TATATTGCCA CTCAGGGTCC TCTAGCGGAG 
ACCACCGAGG ACTTCTGGAG GATGTTATGG GAGCACAATT CCACTATTGT AGTGATGCTG 
ACCAAACTCA GAGAGATGGG ACGGGAGAAG TGTCATCAGT ACTGGCCCGC CGAGCGCTCA 
GCCCGCTATC AGTACTTCGT AGTGGATCCC ATGGCCGAAT ACAACATGCC CCAGTACATC 
CTGCGAGAGT TCAAAGTCAC AGATGCCAGG GACGGTCAAT CCAGAACCAT TCGCCAGTTC 
CAGTTCACTG ATTGGCCAGA GCAGGGAGTG CCAAAAACCG GTGAGGGGTT CATCGATTTC 
ATTGGGCAAG TTCATAAAAC CAAGGAGCAG TTTGGGCAGG ATGGACCAAT CACTGTGCAC 
TGCAGTGCTG GAGTGGGAAG AACAGGTGTT TTCATCACTC TCAGTATTGT CCTGGAGAGG 
ATGAGATATG AGGGGGTGGT GGATCTGTTC CAGACGGTTA AAACCCTGCG CACACAAAGG 
CCTGCCATGG TGCAGACAGA GGACCAGTAC CAGCTGTGCT ACAGAGCCGC TCTGGAGTAC 
CTGGGGAGCT TTGATCACTA TGCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077045.1
CDS	374..6103
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKRKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	NM_001083576.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase receptor type Fb (ptprfb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083576.1

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT CAAGACGTGC ACCTGCTCAG TCTGAGCTCC 
ACCAGCATCA AGGTGAGTTG GGTGGCACCA CCCGCCGCTA GCCGCCACGG CGCAATAGTG 
CGCTACACAG TCAGCTACCA GGCAGTGAAT GGAGAAGACA CAGAGCGCCA TGAGGTGCCG 
GACATTGGAG CGGATGCCTC CAGCTGTGTG TTGGAAGGTC TAGAGAAGTG GACGGAGTAT 
CAGGTGTGGG TGAGAGCGCA CACTGATGTG GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT 
ATCCGAACCA ATGAAGACGT GCCAGGAGCA CCGCCTCGGA AATTGGAAGT GGAGGCTTTG 
AACTCCACCG CTATCCGTGT GACATGGAAA CCTCCTCTTT CTGGAAAGCA GCATGGCCAG 
ATCCGGGGAT ATCAGGTCAT ATACTCCCGG TTGGAGAACG GGGAACCACG GGGACATCCG 
AATATAATGG ATGTCGCTCT GCCAGAAGCA CAGTGGAAAA TTGAAGAGTC TACGGAATAT 
GAGGCTGTGA TCGCTGGACT TGCCTCTGAA ACCTCTTACT CAGTGACTGT GGCTGCGTAC 
ACAACCAAAG GTGATGGAGC TCGCAGCAAA GCCAAAGTGG TCACCACCAC CGGAGCAGTG 
CCTGGCAAAC CCACTATGAT CATCAGCACC ACCGTTGGCA ACACGGCCCT TATCCAGTGG 
CAGCCTCCTA AAGACATGGT GGGTGAGCTG ATGGGCTACC GGCTGCGCTA CAAACGTCTG 
GAAGAGGAAA ACTATGAGAT ACGCGAATTC AGTCGCACCG ACGACCACCA CACCGTCACT 
GACCTGCACA AGGGCGCCAC CTACAGCTTC CACCTGAGCG CCAGAAACCG CGCTGGACTC 
GGAGAGGAGT ACGTTAAAGA CATCGGCACT CCTGAGGACG TGCCCAGCGG GTTTCCTCTT 
AACCTGCAGG TCGTAGGCTT GACTACCTCT ACCACCCGAC TGACCTGGGA GCCTCCAGCG 
TTGTCTGAAC GTAACGGACG GATCATACAT TACATTGTGG TTTACCGAGA CATCAACAGC 
AAACAAAACA GCACCAACCG AACCTCCGAC ACACAGATGA CCATCCAAGG GCTCCGACCT 
GACACCACCT ATGATATCCG TGTGCAGGCG TTCACTAGCA AGGGTGGAGG TCCCATAAGT 
CCCAGTATCC AGAGCAGGAC CATGTCTACG TCACCTGCAT TTGCCACAAG TTTTGGTGTC 
AAAGCTGTGA TGAAAACCTC TGTCCTTCTC ACCTGGGAGG TGCCAGAAAA TTATAAATCA 
CAAGTGCCTT TTAAGATCCT CTATAACCAG CAGAGTGTGG AAGTCCAGGG GAATCTGAAG 
AGGAAGCTGA TCACACGCCT GCAGCCGGAC ACTGATTACT CGTTTGTGCT GATGAGCCGG 
GGAAACAGCG CCGGTGGCCT ACAACAGCAG GTCTCCATCC GCACTGCACC AGACCTGTTC 
AAAACTAAAC CTGTCCTGTA CACGGCAGAC CAGACCAACA ATGGCAAACT CACCATCAAC 
CTGCCCAAAG TGCCCACCTC TGCTCCTGTC AGGTGTTATT ACATTGTGGT CGTGCCCGTC 
TCTCAGACAT CATCTCGTCA GTGGATTAAT CCCGATGAGA TGGAGCTGGA TGAGCTCCTG 
GAGTCTAGTG AGGGAGCTCA TCTCAGGAGA CGTCGACGGC ACACAGACAC CGTAAGACCG 
TACATCGCCG CAAAGCTATC CACGCTCCCA GAAACCTTCA CTCTGGGCGA TGAGCTAGAC 
TACAGCGGCT TCATCAACCG GCCTCTTCCC AACAACCAAC ACTTCCAGTG TTTTGTGATG 
GCTGAACTCA AGGACCAATA CCCTGTTACT GCCAATGAGA AGCAGCGGAC CTTCTCGGCT 
AGCCCATACT CAGACCCCAT CATAGTGCAG GGTGAAAACC AAATGCAGCG CAGTGTGGAC 
CAACCGGAGA TGCTTTGGGT CATGGGCCCT GTGCTCGCTG TGGTGCTCAT CATCATCATA 
GTCATCGCCA TCCTGCTCTT CAAAAGGAAG AGGGCTTCCC CCCTACCAAA AGACGATCAT 
TCGGGTGGTG TGAAGGATTG TCTTCTGGCC AGCTCATCCG ATCCTGTCGA AATGAGGAGA 
CTCAACTACC AGACACCAGG AATGAGGGAA CATCCTCCCA TATCCGTCTC CGAACTGGCA 
GACCACATCG AGCGCCTCAA GGCCAACGAT GCCCTCCGGT TTTCCCAAGA GTATGAGTCT 
ATTGATCCAG GCCAGCAGTT CACATGGGAG AACTCCAACT TGGAGGTCAA CAAGCCAAAG 
AACCGTTATG CCAACGTTAT TGCTTACGAC CACTCGAGAG TTGTTCTCAC CCCTGTGGAC 
GGTGTACCAG GGAGTGACTA CATTAATTCC AACTACATTG ATGGCTACCG TAAGCAGAAT 
GCCTACATTG CCACACAAGG TCCTCTGCCT GAGACTCTGA GCGACTTCTG GAGGATGATT 
TGGGAGCAAC GAGCCAGTAC CATCGTCATG ATGACCAGAC TGGAAGAGAA GTCCAGAGTC 
AAGTGTGACC AATACTGGCC CATTCGTGGC ACAGAGACTT ATGGAATGAT TCAAGTGACC 
ATGCTTGATG CTGTTGAGCT GGCAACCTAC AGTGTGCGCA CCTTTGCCCT TTACAAGAAT 
GGCTCTAGTG AGAAGCGCGA GGTCAGGCAG TTTCAGTTCA TGGCTTGGCC GGACCACGGA 
GTGCCTGAAT ATCCCACCCC CATCCTAGCC TTCCTGCGCA GGGTCAAAGC CTGCAACCCT 
CCTGATGCTG GACCCATGGT AGTGCACTGC AGTGCTGGAG TTGGCCGTAC AGGCTGCTTC 
ATAGTCATCG ATGCGATGTT GGAGCGCATG AAGCACGAGA AGACCGTAGA CATTTATGGC 
CACGTCACGT GCATGAGATC CCAGAGGAAC TATATGGTGC AGACGGAGGA CCAGTACATC 
TTTATCCACG AGGCCCTTCT GGAGGCCGCC ACCTGTGGAA ACACAGAGGT CCCTGCCCGA 
AACCTCTACG CCCACATCCA GAAGCTCACA CAGCCATCGG CTGGAGAGAC CGTCACAGCC 
ATGGAGCTGG AGTTTAAGAG ACTCGCCAAC TCCAAAGCCC ACACCTCAAG ATTCATCAGC 
GCCAACCTGC CCTGCAACAA GTTCAAGAAC CGGCTGGTCA ATATCATGCC TTTCGAGTCC 
ACACGGGTGT GCCTGCAGCC AATCCGAGGA GTGGAGGGGT CCGACTACAT CAATGCCAGC 
TGCATTGACG GATACAGACA GCAGAAGGCC TATATTGCCA CTCAGGGTCC TCTAGCGGAG 
ACCACCGAGG ACTTCTGGAG GATGTTATGG GAGCACAATT CCACTATTGT AGTGATGCTG 
ACCAAACTCA GAGAGATGGG ACGGGAGAAG TGTCATCAGT ACTGGCCCGC CGAGCGCTCA 
GCCCGCTATC AGTACTTCGT AGTGGATCCC ATGGCCGAAT ACAACATGCC CCAGTACATC 
CTGCGAGAGT TCAAAGTCAC AGATGCCAGG GACGGTCAAT CCAGAACCAT TCGCCAGTTC 
CAGTTCACTG ATTGGCCAGA GCAGGGAGTG CCAAAAACCG GTGAGGGGTT CATCGATTTC 
ATTGGGCAAG TTCATAAAAC CAAGGAGCAG TTTGGGCAGG ATGGACCAAT CACTGTGCAC 
TGCAGTGCTG GAGTGGGAAG AACAGGTGTT TTCATCACTC TCAGTATTGT CCTGGAGAGG 
ATGAGATATG AGGGGGTGGT GGATCTGTTC CAGACGGTTA AAACCCTGCG CACACAAAGG 
CCTGCCATGG TGCAGACAGA GGACCAGTAC CAGCTGTGCT ACAGAGCCGC TCTGGAGTAC 
CTGGGGAGCT TTGATCACTA TGCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298458.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296733.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X2, mRNA.

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CloneID	ODa47413
Clone ID Related Accession (Same CDS sequence)	XM_009298458.2 , XM_009298457.3 , XM_021481410.1 , XM_009298457.2 , XM_009298458.3
Accession Version	XM_009298458.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298458.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGTGGAA AATTGAAGAG TCTACGGAAT ATGAGGCTGT GATCGCTGGA 
CTTGCCTCTG AAACCTCTTA CTCAGTGACT GTGGCTGCGT ACACAACCAA AGGTGATGGA 
GCTCGCAGCA AAGCCAAAGT GGTCACCACC ACCGGAGCAG TGCCTGGCAA ACCCACTATG 
ATCATCAGCA CCACCGTTGG CAACACGGCC CTTATCCAGT GGCAGCCTCC TAAAGACATG 
GTGGGTGAGC TGATGGGCTA CCGGCTGCGC TACAAACGTC TGGAAGAGGA AAACTATGAG 
ATACGCGAAT TCAGTCGCAC CGACGACCAC CACACCGTCA CTGACCTGCA CAAGGGCGCC 
ACCTACAGCT TCCACCTGAG CGCCAGAAAC CGCGCTGGAC TCGGAGAGGA GTACGTTAAA 
GACATCGGCA CTCCTGAGGA CGTGCCCAGC GGGTTTCCTC TTAACCTGCA GGTCGTAGGC 
TTGACTACCT CTACCACCCG ACTGACCTGG GAGCCTCCAG CGTTGTCTGA ACGTAACGGA 
CGGATCATAC ATTACATTGT GGTTTACCGA GACATCAACA GCAAACAAAA CAGCACCAAC 
CGAACCTCCG ACACACAGAT GACCATCCAA GGGCTCCGAC CTGACACCAC CTATGATATC 
CGTGTGCAGG CGTTCACTAG CAAGGGTGGA GGTCCCATAA GTCCCAGTAT CCAGAGCAGG 
ACCATGTCTA CGTCACCTGC ATTTGCCACA AGTTTTGGTG TCAAAGCTGT GATGAAAACC 
TCTGTCCTTC TCACCTGGGA GGTGCCAGAA AATTATAAAT CACAAGTGCC TTTTAAGATC 
CTCTATAACC AGCAGAGTGT GGAAGTCCAG GGGAATCTGA AGAGGAAGCT GATCACACGC 
CTGCAGCCGG ACACTGATTA CTCGTTTGTG CTGATGAGCC GGGGAAACAG CGCCGGTGGC 
CTACAACAGC AGGTCTCCAT CCGCACTGCA CCAGACCTGT TCAAAACTAA ACCTGTCCTG 
TACACGGCAG ACCAGACCAA CAATGGCAAA CTCACCATCA ACCTGCCCAA AGTGCCCACC 
TCTGCTCCTG TCAGGTGTTA TTACATTGTG GTCGTGCCCG TCTCTCAGAC ATCATCTCGT 
CAGTGGATTA ATCCCGATGA GATGGAGCTG GATGAGCTCC TGGAGTCTAG TGAGGGAGCT 
CATCTCAGGA GACGTCGACG GCACACAGAC ACCGTAAGAC CGTACATCGC CGCAAAGCTA 
TCCACGCTCC CAGAAACCTT CACTCTGGGC GATGAGCTAG ACTACAGCGG CTTCATCAAC 
CGGCCTCTTC CCAACAACCA ACACTTCCAG TGTTTTGTGA TGGCTGAACT CAAGGACCAA 
TACCCTGTTA CTGCCAATGA GAAGCAGCGG ACCTTCTCGG CTAGCCCATA CTCAGACCCC 
ATCATAGTGC AGGGTGAAAA CCAAATGCAG CGCAGTGTGG ACCAACCGGA GATGCTTTGG 
GTCATGGGCC CTGTGCTCGC TGTGGTGCTC ATCATCATCA TAGTCATCGC CATCCTGCTC 
TTCAAAAGCA AACAGGAGAG GAAGAGGGCT TCCCCCCTAC CAAAAGACGA TCATTCGGGT 
GGTGTGAAGG ATTGTCTTCT GGCCAGCTCA TCCGATCCTG TCGAAATGAG GAGACTCAAC 
TACCAGACAC CAGGAATGAG GGAACATCCT CCCATATCCG TCTCCGAACT GGCAGACCAC 
ATCGAGCGCC TCAAGGCCAA CGATGCCCTC CGGTTTTCCC AAGAGTATGA GTCTATTGAT 
CCAGGCCAGC AGTTCACATG GGAGAACTCC AACTTGGAGG TCAACAAGCC AAAGAACCGT 
TATGCCAACG TTATTGCTTA CGACCACTCG AGAGTTGTTC TCACCCCTGT GGACGGTGTA 
CCAGGGAGTG ACTACATTAA TTCCAACTAC ATTGATGGCT ACCGTAAGCA GAATGCCTAC 
ATTGCCACAC AAGGTCCTCT GCCTGAGACT CTGAGCGACT TCTGGAGGAT GATTTGGGAG 
CAACGAGCCA GTACCATCGT CATGATGACC AGACTGGAAG AGAAGTCCAG AGTCAAGTGT 
GACCAATACT GGCCCATTCG TGGCACAGAG ACTTATGGAA TGATTCAAGT GACCATGCTT 
GATGCTGTTG AGCTGGCAAC CTACAGTGTG CGCACCTTTG CCCTTTACAA GAATGGCTCT 
AGTGAGAAGC GCGAGGTCAG GCAGTTTCAG TTCATGGCTT GGCCGGACCA CGGAGTGCCT 
GAATATCCCA CCCCCATCCT AGCCTTCCTG CGCAGGGTCA AAGCCTGCAA CCCTCCTGAT 
GCTGGACCCA TGGTAGTGCA CTGCAGTGCT GGAGTTGGCC GTACAGGCTG CTTCATAGTC 
ATCGATGCGA TGTTGGAGCG CATGAAGCAC GAGAAGACCG TAGACATTTA TGGCCACGTC 
ACGTGCATGA GATCCCAGAG GAACTATATG GTGCAGACGG AGGACCAGTA CATCTTTATC 
CACGAGGCCC TTCTGGAGGC CGCCACCTGT GGAAACACAG AGGTCCCTGC CCGAAACCTC 
TACGCCCACA TCCAGAAGCT CACACAGCCA TCGGCTGGAG AGACCGTCAC AGCCATGGAG 
CTGGAGTTTA AGAGACTCGC CAACTCCAAA GCCCACACCT CAAGATTCAT CAGCGCCAAC 
CTGCCCTGCA ACAAGTTCAA GAACCGGCTG GTCAATATCA TGCCTTTCGA GTCCACACGG 
GTGTGCCTGC AGCCAATCCG AGGAGTGGAG GGGTCCGACT ACATCAATGC CAGCTGCATT 
GACGGATACA GACAGCAGAA GGCCTATATT GCCACTCAGG GTCCTCTAGC GGAGACCACC 
GAGGACTTCT GGAGGATGTT ATGGGAGCAC AATTCCACTA TTGTAGTGAT GCTGACCAAA 
CTCAGAGAGA TGGGACGGGA GAAGTGTCAT CAGTACTGGC CCGCCGAGCG CTCAGCCCGC 
TATCAGTACT TCGTAGTGGA TCCCATGGCC GAATACAACA TGCCCCAGTA CATCCTGCGA 
GAGTTCAAAG TCACAGATGC CAGGGACGGT CAATCCAGAA CCATTCGCCA GTTCCAGTTC 
ACTGATTGGC CAGAGCAGGG AGTGCCAAAA ACCGGTGAGG GGTTCATCGA TTTCATTGGG 
CAAGTTCATA AAACCAAGGA GCAGTTTGGG CAGGATGGAC CAATCACTGT GCACTGCAGT 
GCTGGAGTGG GAAGAACAGG TGTTTTCATC ACTCTCAGTA TTGTCCTGGA GAGGATGAGA 
TATGAGGGGG TGGTGGATCT GTTCCAGACG GTTAAAACCC TGCGCACACA AAGGCCTGCC 
ATGGTGCAGA CAGAGGACCA GTACCAGCTG TGCTACAGAG CCGCTCTGGA GTACCTGGGG 
AGCTTTGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296733.1
CDS	271..6054
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVIREDPRGVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298458.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298458.2

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGTGGAA AATTGAAGAG TCTACGGAAT ATGAGGCTGT GATCGCTGGA 
CTTGCCTCTG AAACCTCTTA CTCAGTGACT GTGGCTGCGT ACACAACCAA AGGTGATGGA 
GCTCGCAGCA AAGCCAAAGT GGTCACCACC ACCGGAGCAG TGCCTGGCAA ACCCACTATG 
ATCATCAGCA CCACCGTTGG CAACACGGCC CTTATCCAGT GGCAGCCTCC TAAAGACATG 
GTGGGTGAGC TGATGGGCTA CCGGCTGCGC TACAAACGTC TGGAAGAGGA AAACTATGAG 
ATACGCGAAT TCAGTCGCAC CGACGACCAC CACACCGTCA CTGACCTGCA CAAGGGCGCC 
ACCTACAGCT TCCACCTGAG CGCCAGAAAC CGCGCTGGAC TCGGAGAGGA GTACGTTAAA 
GACATCGGCA CTCCTGAGGA CGTGCCCAGC GGGTTTCCTC TTAACCTGCA GGTCGTAGGC 
TTGACTACCT CTACCACCCG ACTGACCTGG GAGCCTCCAG CGTTGTCTGA ACGTAACGGA 
CGGATCATAC ATTACATTGT GGTTTACCGA GACATCAACA GCAAACAAAA CAGCACCAAC 
CGAACCTCCG ACACACAGAT GACCATCCAA GGGCTCCGAC CTGACACCAC CTATGATATC 
CGTGTGCAGG CGTTCACTAG CAAGGGTGGA GGTCCCATAA GTCCCAGTAT CCAGAGCAGG 
ACCATGTCTA CGTCACCTGC ATTTGCCACA AGTTTTGGTG TCAAAGCTGT GATGAAAACC 
TCTGTCCTTC TCACCTGGGA GGTGCCAGAA AATTATAAAT CACAAGTGCC TTTTAAGATC 
CTCTATAACC AGCAGAGTGT GGAAGTCCAG GGGAATCTGA AGAGGAAGCT GATCACACGC 
CTGCAGCCGG ACACTGATTA CTCGTTTGTG CTGATGAGCC GGGGAAACAG CGCCGGTGGC 
CTACAACAGC AGGTCTCCAT CCGCACTGCA CCAGACCTGT TCAAAACTAA ACCTGTCCTG 
TACACGGCAG ACCAGACCAA CAATGGCAAA CTCACCATCA ACCTGCCCAA AGTGCCCACC 
TCTGCTCCTG TCAGGTGTTA TTACATTGTG GTCGTGCCCG TCTCTCAGAC ATCATCTCGT 
CAGTGGATTA ATCCCGATGA GATGGAGCTG GATGAGCTCC TGGAGTCTAG TGAGGGAGCT 
CATCTCAGGA GACGTCGACG GCACACAGAC ACCGTAAGAC CGTACATCGC CGCAAAGCTA 
TCCACGCTCC CAGAAACCTT CACTCTGGGC GATGAGCTAG ACTACAGCGG CTTCATCAAC 
CGGCCTCTTC CCAACAACCA ACACTTCCAG TGTTTTGTGA TGGCTGAACT CAAGGACCAA 
TACCCTGTTA CTGCCAATGA GAAGCAGCGG ACCTTCTCGG CTAGCCCATA CTCAGACCCC 
ATCATAGTGC AGGGTGAAAA CCAAATGCAG CGCAGTGTGG ACCAACCGGA GATGCTTTGG 
GTCATGGGCC CTGTGCTCGC TGTGGTGCTC ATCATCATCA TAGTCATCGC CATCCTGCTC 
TTCAAAAGCA AACAGGAGAG GAAGAGGGCT TCCCCCCTAC CAAAAGACGA TCATTCGGGT 
GGTGTGAAGG ATTGTCTTCT GGCCAGCTCA TCCGATCCTG TCGAAATGAG GAGACTCAAC 
TACCAGACAC CAGGAATGAG GGAACATCCT CCCATATCCG TCTCCGAACT GGCAGACCAC 
ATCGAGCGCC TCAAGGCCAA CGATGCCCTC CGGTTTTCCC AAGAGTATGA GTCTATTGAT 
CCAGGCCAGC AGTTCACATG GGAGAACTCC AACTTGGAGG TCAACAAGCC AAAGAACCGT 
TATGCCAACG TTATTGCTTA CGACCACTCG AGAGTTGTTC TCACCCCTGT GGACGGTGTA 
CCAGGGAGTG ACTACATTAA TTCCAACTAC ATTGATGGCT ACCGTAAGCA GAATGCCTAC 
ATTGCCACAC AAGGTCCTCT GCCTGAGACT CTGAGCGACT TCTGGAGGAT GATTTGGGAG 
CAACGAGCCA GTACCATCGT CATGATGACC AGACTGGAAG AGAAGTCCAG AGTCAAGTGT 
GACCAATACT GGCCCATTCG TGGCACAGAG ACTTATGGAA TGATTCAAGT GACCATGCTT 
GATGCTGTTG AGCTGGCAAC CTACAGTGTG CGCACCTTTG CCCTTTACAA GAATGGCTCT 
AGTGAGAAGC GCGAGGTCAG GCAGTTTCAG TTCATGGCTT GGCCGGACCA CGGAGTGCCT 
GAATATCCCA CCCCCATCCT AGCCTTCCTG CGCAGGGTCA AAGCCTGCAA CCCTCCTGAT 
GCTGGACCCA TGGTAGTGCA CTGCAGTGCT GGAGTTGGCC GTACAGGCTG CTTCATAGTC 
ATCGATGCGA TGTTGGAGCG CATGAAGCAC GAGAAGACCG TAGACATTTA TGGCCACGTC 
ACGTGCATGA GATCCCAGAG GAACTATATG GTGCAGACGG AGGACCAGTA CATCTTTATC 
CACGAGGCCC TTCTGGAGGC CGCCACCTGT GGAAACACAG AGGTCCCTGC CCGAAACCTC 
TACGCCCACA TCCAGAAGCT CACACAGCCA TCGGCTGGAG AGACCGTCAC AGCCATGGAG 
CTGGAGTTTA AGAGACTCGC CAACTCCAAA GCCCACACCT CAAGATTCAT CAGCGCCAAC 
CTGCCCTGCA ACAAGTTCAA GAACCGGCTG GTCAATATCA TGCCTTTCGA GTCCACACGG 
GTGTGCCTGC AGCCAATCCG AGGAGTGGAG GGGTCCGACT ACATCAATGC CAGCTGCATT 
GACGGATACA GACAGCAGAA GGCCTATATT GCCACTCAGG GTCCTCTAGC GGAGACCACC 
GAGGACTTCT GGAGGATGTT ATGGGAGCAC AATTCCACTA TTGTAGTGAT GCTGACCAAA 
CTCAGAGAGA TGGGACGGGA GAAGTGTCAT CAGTACTGGC CCGCCGAGCG CTCAGCCCGC 
TATCAGTACT TCGTAGTGGA TCCCATGGCC GAATACAACA TGCCCCAGTA CATCCTGCGA 
GAGTTCAAAG TCACAGATGC CAGGGACGGT CAATCCAGAA CCATTCGCCA GTTCCAGTTC 
ACTGATTGGC CAGAGCAGGG AGTGCCAAAA ACCGGTGAGG GGTTCATCGA TTTCATTGGG 
CAAGTTCATA AAACCAAGGA GCAGTTTGGG CAGGATGGAC CAATCACTGT GCACTGCAGT 
GCTGGAGTGG GAAGAACAGG TGTTTTCATC ACTCTCAGTA TTGTCCTGGA GAGGATGAGA 
TATGAGGGGG TGGTGGATCT GTTCCAGACG GTTAAAACCC TGCGCACACA AAGGCCTGCC 
ATGGTGCAGA CAGAGGACCA GTACCAGCTG TGCTACAGAG CCGCTCTGGA GTACCTGGGG 
AGCTTTGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298457.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296732.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X1, mRNA.

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CloneID	ODa47413
Clone ID Related Accession (Same CDS sequence)	XM_009298458.2 , XM_009298457.3 , XM_021481410.1 , XM_009298457.2 , XM_009298458.3
Accession Version	XM_009298457.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298457.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGTGGAA AATTGAAGAG TCTACGGAAT ATGAGGCTGT GATCGCTGGA 
CTTGCCTCTG AAACCTCTTA CTCAGTGACT GTGGCTGCGT ACACAACCAA AGGTGATGGA 
GCTCGCAGCA AAGCCAAAGT GGTCACCACC ACCGGAGCAG TGCCTGGCAA ACCCACTATG 
ATCATCAGCA CCACCGTTGG CAACACGGCC CTTATCCAGT GGCAGCCTCC TAAAGACATG 
GTGGGTGAGC TGATGGGCTA CCGGCTGCGC TACAAACGTC TGGAAGAGGA AAACTATGAG 
ATACGCGAAT TCAGTCGCAC CGACGACCAC CACACCGTCA CTGACCTGCA CAAGGGCGCC 
ACCTACAGCT TCCACCTGAG CGCCAGAAAC CGCGCTGGAC TCGGAGAGGA GTACGTTAAA 
GACATCGGCA CTCCTGAGGA CGTGCCCAGC GGGTTTCCTC TTAACCTGCA GGTCGTAGGC 
TTGACTACCT CTACCACCCG ACTGACCTGG GAGCCTCCAG CGTTGTCTGA ACGTAACGGA 
CGGATCATAC ATTACATTGT GGTTTACCGA GACATCAACA GCAAACAAAA CAGCACCAAC 
CGAACCTCCG ACACACAGAT GACCATCCAA GGGCTCCGAC CTGACACCAC CTATGATATC 
CGTGTGCAGG CGTTCACTAG CAAGGGTGGA GGTCCCATAA GTCCCAGTAT CCAGAGCAGG 
ACCATGTCTA CGTCACCTGC ATTTGCCACA AGTTTTGGTG TCAAAGCTGT GATGAAAACC 
TCTGTCCTTC TCACCTGGGA GGTGCCAGAA AATTATAAAT CACAAGTGCC TTTTAAGATC 
CTCTATAACC AGCAGAGTGT GGAAGTCCAG GGGAATCTGA AGAGGAAGCT GATCACACGC 
CTGCAGCCGG ACACTGATTA CTCGTTTGTG CTGATGAGCC GGGGAAACAG CGCCGGTGGC 
CTACAACAGC AGGTCTCCAT CCGCACTGCA CCAGACCTGT TCAAAACTAA ACCTGTCCTG 
TACACGGCAG ACCAGACCAA CAATGGCAAA CTCACCATCA ACCTGCCCAA AGTGCCCACC 
TCTGCTCCTG TCAGGTGTTA TTACATTGTG GTCGTGCCCG TCTCTCAGAC ATCATCTCGT 
CAGTGGATTA ATCCCGATGA GATGGAGCTG GATGAGCTCC TGGAGTCTAG TGAGGGAGCT 
CATCTCAGGA GACGTCGACG GCACACAGAC ACCGTAAGAC CGTACATCGC CGCAAAGCTA 
TCCACGCTCC CAGAAACCTT CACTCTGGGC GATGAGCTAG ACTACAGCGG CTTCATCAAC 
CGGCCTCTTC CCAACAACCA ACACTTCCAG TGTTTTGTGA TGGCTGAACT CAAGGACCAA 
TACCCTGTTA CTGCCAATGA GAAGCAGCGG ACCTTCTCGG CTAGCCCATA CTCAGACCCC 
ATCATAGTGC AGGGTGAAAA CCAAATGCAG CGCAGTGTGG ACCAACCGGA GATGCTTTGG 
GTCATGGGCC CTGTGCTCGC TGTGGTGCTC ATCATCATCA TAGTCATCGC CATCCTGCTC 
TTCAAAAGCA AACAGGAGAG GAAGAGGGCT TCCCCCCTAC CAAAAGACGA TCATTCGGGT 
GGTGTGAAGG ATTGTCTTCT GGCCAGCTCA TCCGATCCTG TCGAAATGAG GAGACTCAAC 
TACCAGACAC CAGGAATGAG GGAACATCCT CCCATATCCG TCTCCGAACT GGCAGACCAC 
ATCGAGCGCC TCAAGGCCAA CGATGCCCTC CGGTTTTCCC AAGAGTATGA GTCTATTGAT 
CCAGGCCAGC AGTTCACATG GGAGAACTCC AACTTGGAGG TCAACAAGCC AAAGAACCGT 
TATGCCAACG TTATTGCTTA CGACCACTCG AGAGTTGTTC TCACCCCTGT GGACGGTGTA 
CCAGGGAGTG ACTACATTAA TTCCAACTAC ATTGATGGCT ACCGTAAGCA GAATGCCTAC 
ATTGCCACAC AAGGTCCTCT GCCTGAGACT CTGAGCGACT TCTGGAGGAT GATTTGGGAG 
CAACGAGCCA GTACCATCGT CATGATGACC AGACTGGAAG AGAAGTCCAG AGTCAAGTGT 
GACCAATACT GGCCCATTCG TGGCACAGAG ACTTATGGAA TGATTCAAGT GACCATGCTT 
GATGCTGTTG AGCTGGCAAC CTACAGTGTG CGCACCTTTG CCCTTTACAA GAATGGCTCT 
AGTGAGAAGC GCGAGGTCAG GCAGTTTCAG TTCATGGCTT GGCCGGACCA CGGAGTGCCT 
GAATATCCCA CCCCCATCCT AGCCTTCCTG CGCAGGGTCA AAGCCTGCAA CCCTCCTGAT 
GCTGGACCCA TGGTAGTGCA CTGCAGTGCT GGAGTTGGCC GTACAGGCTG CTTCATAGTC 
ATCGATGCGA TGTTGGAGCG CATGAAGCAC GAGAAGACCG TAGACATTTA TGGCCACGTC 
ACGTGCATGA GATCCCAGAG GAACTATATG GTGCAGACGG AGGACCAGTA CATCTTTATC 
CACGAGGCCC TTCTGGAGGC CGCCACCTGT GGAAACACAG AGGTCCCTGC CCGAAACCTC 
TACGCCCACA TCCAGAAGCT CACACAGCCA TCGGCTGGAG AGACCGTCAC AGCCATGGAG 
CTGGAGTTTA AGAGACTCGC CAACTCCAAA GCCCACACCT CAAGATTCAT CAGCGCCAAC 
CTGCCCTGCA ACAAGTTCAA GAACCGGCTG GTCAATATCA TGCCTTTCGA GTCCACACGG 
GTGTGCCTGC AGCCAATCCG AGGAGTGGAG GGGTCCGACT ACATCAATGC CAGCTGCATT 
GACGGATACA GACAGCAGAA GGCCTATATT GCCACTCAGG GTCCTCTAGC GGAGACCACC 
GAGGACTTCT GGAGGATGTT ATGGGAGCAC AATTCCACTA TTGTAGTGAT GCTGACCAAA 
CTCAGAGAGA TGGGACGGGA GAAGTGTCAT CAGTACTGGC CCGCCGAGCG CTCAGCCCGC 
TATCAGTACT TCGTAGTGGA TCCCATGGCC GAATACAACA TGCCCCAGTA CATCCTGCGA 
GAGTTCAAAG TCACAGATGC CAGGGACGGT CAATCCAGAA CCATTCGCCA GTTCCAGTTC 
ACTGATTGGC CAGAGCAGGG AGTGCCAAAA ACCGGTGAGG GGTTCATCGA TTTCATTGGG 
CAAGTTCATA AAACCAAGGA GCAGTTTGGG CAGGATGGAC CAATCACTGT GCACTGCAGT 
GCTGGAGTGG GAAGAACAGG TGTTTTCATC ACTCTCAGTA TTGTCCTGGA GAGGATGAGA 
TATGAGGGGG TGGTGGATCT GTTCCAGACG GTTAAAACCC TGCGCACACA AAGGCCTGCC 
ATGGTGCAGA CAGAGGACCA GTACCAGCTG TGCTACAGAG CCGCTCTGGA GTACCTGGGG 
AGCTTTGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296732.1
CDS	422..6205
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVIREDPRGVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298457.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298457.3

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGTGGAA AATTGAAGAG TCTACGGAAT ATGAGGCTGT GATCGCTGGA 
CTTGCCTCTG AAACCTCTTA CTCAGTGACT GTGGCTGCGT ACACAACCAA AGGTGATGGA 
GCTCGCAGCA AAGCCAAAGT GGTCACCACC ACCGGAGCAG TGCCTGGCAA ACCCACTATG 
ATCATCAGCA CCACCGTTGG CAACACGGCC CTTATCCAGT GGCAGCCTCC TAAAGACATG 
GTGGGTGAGC TGATGGGCTA CCGGCTGCGC TACAAACGTC TGGAAGAGGA AAACTATGAG 
ATACGCGAAT TCAGTCGCAC CGACGACCAC CACACCGTCA CTGACCTGCA CAAGGGCGCC 
ACCTACAGCT TCCACCTGAG CGCCAGAAAC CGCGCTGGAC TCGGAGAGGA GTACGTTAAA 
GACATCGGCA CTCCTGAGGA CGTGCCCAGC GGGTTTCCTC TTAACCTGCA GGTCGTAGGC 
TTGACTACCT CTACCACCCG ACTGACCTGG GAGCCTCCAG CGTTGTCTGA ACGTAACGGA 
CGGATCATAC ATTACATTGT GGTTTACCGA GACATCAACA GCAAACAAAA CAGCACCAAC 
CGAACCTCCG ACACACAGAT GACCATCCAA GGGCTCCGAC CTGACACCAC CTATGATATC 
CGTGTGCAGG CGTTCACTAG CAAGGGTGGA GGTCCCATAA GTCCCAGTAT CCAGAGCAGG 
ACCATGTCTA CGTCACCTGC ATTTGCCACA AGTTTTGGTG TCAAAGCTGT GATGAAAACC 
TCTGTCCTTC TCACCTGGGA GGTGCCAGAA AATTATAAAT CACAAGTGCC TTTTAAGATC 
CTCTATAACC AGCAGAGTGT GGAAGTCCAG GGGAATCTGA AGAGGAAGCT GATCACACGC 
CTGCAGCCGG ACACTGATTA CTCGTTTGTG CTGATGAGCC GGGGAAACAG CGCCGGTGGC 
CTACAACAGC AGGTCTCCAT CCGCACTGCA CCAGACCTGT TCAAAACTAA ACCTGTCCTG 
TACACGGCAG ACCAGACCAA CAATGGCAAA CTCACCATCA ACCTGCCCAA AGTGCCCACC 
TCTGCTCCTG TCAGGTGTTA TTACATTGTG GTCGTGCCCG TCTCTCAGAC ATCATCTCGT 
CAGTGGATTA ATCCCGATGA GATGGAGCTG GATGAGCTCC TGGAGTCTAG TGAGGGAGCT 
CATCTCAGGA GACGTCGACG GCACACAGAC ACCGTAAGAC CGTACATCGC CGCAAAGCTA 
TCCACGCTCC CAGAAACCTT CACTCTGGGC GATGAGCTAG ACTACAGCGG CTTCATCAAC 
CGGCCTCTTC CCAACAACCA ACACTTCCAG TGTTTTGTGA TGGCTGAACT CAAGGACCAA 
TACCCTGTTA CTGCCAATGA GAAGCAGCGG ACCTTCTCGG CTAGCCCATA CTCAGACCCC 
ATCATAGTGC AGGGTGAAAA CCAAATGCAG CGCAGTGTGG ACCAACCGGA GATGCTTTGG 
GTCATGGGCC CTGTGCTCGC TGTGGTGCTC ATCATCATCA TAGTCATCGC CATCCTGCTC 
TTCAAAAGCA AACAGGAGAG GAAGAGGGCT TCCCCCCTAC CAAAAGACGA TCATTCGGGT 
GGTGTGAAGG ATTGTCTTCT GGCCAGCTCA TCCGATCCTG TCGAAATGAG GAGACTCAAC 
TACCAGACAC CAGGAATGAG GGAACATCCT CCCATATCCG TCTCCGAACT GGCAGACCAC 
ATCGAGCGCC TCAAGGCCAA CGATGCCCTC CGGTTTTCCC AAGAGTATGA GTCTATTGAT 
CCAGGCCAGC AGTTCACATG GGAGAACTCC AACTTGGAGG TCAACAAGCC AAAGAACCGT 
TATGCCAACG TTATTGCTTA CGACCACTCG AGAGTTGTTC TCACCCCTGT GGACGGTGTA 
CCAGGGAGTG ACTACATTAA TTCCAACTAC ATTGATGGCT ACCGTAAGCA GAATGCCTAC 
ATTGCCACAC AAGGTCCTCT GCCTGAGACT CTGAGCGACT TCTGGAGGAT GATTTGGGAG 
CAACGAGCCA GTACCATCGT CATGATGACC AGACTGGAAG AGAAGTCCAG AGTCAAGTGT 
GACCAATACT GGCCCATTCG TGGCACAGAG ACTTATGGAA TGATTCAAGT GACCATGCTT 
GATGCTGTTG AGCTGGCAAC CTACAGTGTG CGCACCTTTG CCCTTTACAA GAATGGCTCT 
AGTGAGAAGC GCGAGGTCAG GCAGTTTCAG TTCATGGCTT GGCCGGACCA CGGAGTGCCT 
GAATATCCCA CCCCCATCCT AGCCTTCCTG CGCAGGGTCA AAGCCTGCAA CCCTCCTGAT 
GCTGGACCCA TGGTAGTGCA CTGCAGTGCT GGAGTTGGCC GTACAGGCTG CTTCATAGTC 
ATCGATGCGA TGTTGGAGCG CATGAAGCAC GAGAAGACCG TAGACATTTA TGGCCACGTC 
ACGTGCATGA GATCCCAGAG GAACTATATG GTGCAGACGG AGGACCAGTA CATCTTTATC 
CACGAGGCCC TTCTGGAGGC CGCCACCTGT GGAAACACAG AGGTCCCTGC CCGAAACCTC 
TACGCCCACA TCCAGAAGCT CACACAGCCA TCGGCTGGAG AGACCGTCAC AGCCATGGAG 
CTGGAGTTTA AGAGACTCGC CAACTCCAAA GCCCACACCT CAAGATTCAT CAGCGCCAAC 
CTGCCCTGCA ACAAGTTCAA GAACCGGCTG GTCAATATCA TGCCTTTCGA GTCCACACGG 
GTGTGCCTGC AGCCAATCCG AGGAGTGGAG GGGTCCGACT ACATCAATGC CAGCTGCATT 
GACGGATACA GACAGCAGAA GGCCTATATT GCCACTCAGG GTCCTCTAGC GGAGACCACC 
GAGGACTTCT GGAGGATGTT ATGGGAGCAC AATTCCACTA TTGTAGTGAT GCTGACCAAA 
CTCAGAGAGA TGGGACGGGA GAAGTGTCAT CAGTACTGGC CCGCCGAGCG CTCAGCCCGC 
TATCAGTACT TCGTAGTGGA TCCCATGGCC GAATACAACA TGCCCCAGTA CATCCTGCGA 
GAGTTCAAAG TCACAGATGC CAGGGACGGT CAATCCAGAA CCATTCGCCA GTTCCAGTTC 
ACTGATTGGC CAGAGCAGGG AGTGCCAAAA ACCGGTGAGG GGTTCATCGA TTTCATTGGG 
CAAGTTCATA AAACCAAGGA GCAGTTTGGG CAGGATGGAC CAATCACTGT GCACTGCAGT 
GCTGGAGTGG GAAGAACAGG TGTTTTCATC ACTCTCAGTA TTGTCCTGGA GAGGATGAGA 
TATGAGGGGG TGGTGGATCT GTTCCAGACG GTTAAAACCC TGCGCACACA AAGGCCTGCC 
ATGGTGCAGA CAGAGGACCA GTACCAGCTG TGCTACAGAG CCGCTCTGGA GTACCTGGGG 
AGCTTTGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_021481410.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337085.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X2, mRNA.

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CloneID	ODa47413
Clone ID Related Accession (Same CDS sequence)	XM_009298458.2 , XM_009298457.3 , XM_021481410.1 , XM_009298457.2 , XM_009298458.3
Accession Version	XM_021481410.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGTGGAA AATTGAAGAG TCTACGGAAT ATGAGGCTGT GATCGCTGGA 
CTTGCCTCTG AAACCTCTTA CTCAGTGACT GTGGCTGCGT ACACAACCAA AGGTGATGGA 
GCTCGCAGCA AAGCCAAAGT GGTCACCACC ACCGGAGCAG TGCCTGGCAA ACCCACTATG 
ATCATCAGCA CCACCGTTGG CAACACGGCC CTTATCCAGT GGCAGCCTCC TAAAGACATG 
GTGGGTGAGC TGATGGGCTA CCGGCTGCGC TACAAACGTC TGGAAGAGGA AAACTATGAG 
ATACGCGAAT TCAGTCGCAC CGACGACCAC CACACCGTCA CTGACCTGCA CAAGGGCGCC 
ACCTACAGCT TCCACCTGAG CGCCAGAAAC CGCGCTGGAC TCGGAGAGGA GTACGTTAAA 
GACATCGGCA CTCCTGAGGA CGTGCCCAGC GGGTTTCCTC TTAACCTGCA GGTCGTAGGC 
TTGACTACCT CTACCACCCG ACTGACCTGG GAGCCTCCAG CGTTGTCTGA ACGTAACGGA 
CGGATCATAC ATTACATTGT GGTTTACCGA GACATCAACA GCAAACAAAA CAGCACCAAC 
CGAACCTCCG ACACACAGAT GACCATCCAA GGGCTCCGAC CTGACACCAC CTATGATATC 
CGTGTGCAGG CGTTCACTAG CAAGGGTGGA GGTCCCATAA GTCCCAGTAT CCAGAGCAGG 
ACCATGTCTA CGTCACCTGC ATTTGCCACA AGTTTTGGTG TCAAAGCTGT GATGAAAACC 
TCTGTCCTTC TCACCTGGGA GGTGCCAGAA AATTATAAAT CACAAGTGCC TTTTAAGATC 
CTCTATAACC AGCAGAGTGT GGAAGTCCAG GGGAATCTGA AGAGGAAGCT GATCACACGC 
CTGCAGCCGG ACACTGATTA CTCGTTTGTG CTGATGAGCC GGGGAAACAG CGCCGGTGGC 
CTACAACAGC AGGTCTCCAT CCGCACTGCA CCAGACCTGT TCAAAACTAA ACCTGTCCTG 
TACACGGCAG ACCAGACCAA CAATGGCAAA CTCACCATCA ACCTGCCCAA AGTGCCCACC 
TCTGCTCCTG TCAGGTGTTA TTACATTGTG GTCGTGCCCG TCTCTCAGAC ATCATCTCGT 
CAGTGGATTA ATCCCGATGA GATGGAGCTG GATGAGCTCC TGGAGTCTAG TGAGGGAGCT 
CATCTCAGGA GACGTCGACG GCACACAGAC ACCGTAAGAC CGTACATCGC CGCAAAGCTA 
TCCACGCTCC CAGAAACCTT CACTCTGGGC GATGAGCTAG ACTACAGCGG CTTCATCAAC 
CGGCCTCTTC CCAACAACCA ACACTTCCAG TGTTTTGTGA TGGCTGAACT CAAGGACCAA 
TACCCTGTTA CTGCCAATGA GAAGCAGCGG ACCTTCTCGG CTAGCCCATA CTCAGACCCC 
ATCATAGTGC AGGGTGAAAA CCAAATGCAG CGCAGTGTGG ACCAACCGGA GATGCTTTGG 
GTCATGGGCC CTGTGCTCGC TGTGGTGCTC ATCATCATCA TAGTCATCGC CATCCTGCTC 
TTCAAAAGCA AACAGGAGAG GAAGAGGGCT TCCCCCCTAC CAAAAGACGA TCATTCGGGT 
GGTGTGAAGG ATTGTCTTCT GGCCAGCTCA TCCGATCCTG TCGAAATGAG GAGACTCAAC 
TACCAGACAC CAGGAATGAG GGAACATCCT CCCATATCCG TCTCCGAACT GGCAGACCAC 
ATCGAGCGCC TCAAGGCCAA CGATGCCCTC CGGTTTTCCC AAGAGTATGA GTCTATTGAT 
CCAGGCCAGC AGTTCACATG GGAGAACTCC AACTTGGAGG TCAACAAGCC AAAGAACCGT 
TATGCCAACG TTATTGCTTA CGACCACTCG AGAGTTGTTC TCACCCCTGT GGACGGTGTA 
CCAGGGAGTG ACTACATTAA TTCCAACTAC ATTGATGGCT ACCGTAAGCA GAATGCCTAC 
ATTGCCACAC AAGGTCCTCT GCCTGAGACT CTGAGCGACT TCTGGAGGAT GATTTGGGAG 
CAACGAGCCA GTACCATCGT CATGATGACC AGACTGGAAG AGAAGTCCAG AGTCAAGTGT 
GACCAATACT GGCCCATTCG TGGCACAGAG ACTTATGGAA TGATTCAAGT GACCATGCTT 
GATGCTGTTG AGCTGGCAAC CTACAGTGTG CGCACCTTTG CCCTTTACAA GAATGGCTCT 
AGTGAGAAGC GCGAGGTCAG GCAGTTTCAG TTCATGGCTT GGCCGGACCA CGGAGTGCCT 
GAATATCCCA CCCCCATCCT AGCCTTCCTG CGCAGGGTCA AAGCCTGCAA CCCTCCTGAT 
GCTGGACCCA TGGTAGTGCA CTGCAGTGCT GGAGTTGGCC GTACAGGCTG CTTCATAGTC 
ATCGATGCGA TGTTGGAGCG CATGAAGCAC GAGAAGACCG TAGACATTTA TGGCCACGTC 
ACGTGCATGA GATCCCAGAG GAACTATATG GTGCAGACGG AGGACCAGTA CATCTTTATC 
CACGAGGCCC TTCTGGAGGC CGCCACCTGT GGAAACACAG AGGTCCCTGC CCGAAACCTC 
TACGCCCACA TCCAGAAGCT CACACAGCCA TCGGCTGGAG AGACCGTCAC AGCCATGGAG 
CTGGAGTTTA AGAGACTCGC CAACTCCAAA GCCCACACCT CAAGATTCAT CAGCGCCAAC 
CTGCCCTGCA ACAAGTTCAA GAACCGGCTG GTCAATATCA TGCCTTTCGA GTCCACACGG 
GTGTGCCTGC AGCCAATCCG AGGAGTGGAG GGGTCCGACT ACATCAATGC CAGCTGCATT 
GACGGATACA GACAGCAGAA GGCCTATATT GCCACTCAGG GTCCTCTAGC GGAGACCACC 
GAGGACTTCT GGAGGATGTT ATGGGAGCAC AATTCCACTA TTGTAGTGAT GCTGACCAAA 
CTCAGAGAGA TGGGACGGGA GAAGTGTCAT CAGTACTGGC CCGCCGAGCG CTCAGCCCGC 
TATCAGTACT TCGTAGTGGA TCCCATGGCC GAATACAACA TGCCCCAGTA CATCCTGCGA 
GAGTTCAAAG TCACAGATGC CAGGGACGGT CAATCCAGAA CCATTCGCCA GTTCCAGTTC 
ACTGATTGGC CAGAGCAGGG AGTGCCAAAA ACCGGTGAGG GGTTCATCGA TTTCATTGGG 
CAAGTTCATA AAACCAAGGA GCAGTTTGGG CAGGATGGAC CAATCACTGT GCACTGCAGT 
GCTGGAGTGG GAAGAACAGG TGTTTTCATC ACTCTCAGTA TTGTCCTGGA GAGGATGAGA 
TATGAGGGGG TGGTGGATCT GTTCCAGACG GTTAAAACCC TGCGCACACA AAGGCCTGCC 
ATGGTGCAGA CAGAGGACCA GTACCAGCTG TGCTACAGAG CCGCTCTGGA GTACCTGGGG 
AGCTTTGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337085.1
CDS	747..6530
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVIREDPRGVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021481410.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481410.1

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGTGGAA AATTGAAGAG TCTACGGAAT ATGAGGCTGT GATCGCTGGA 
CTTGCCTCTG AAACCTCTTA CTCAGTGACT GTGGCTGCGT ACACAACCAA AGGTGATGGA 
GCTCGCAGCA AAGCCAAAGT GGTCACCACC ACCGGAGCAG TGCCTGGCAA ACCCACTATG 
ATCATCAGCA CCACCGTTGG CAACACGGCC CTTATCCAGT GGCAGCCTCC TAAAGACATG 
GTGGGTGAGC TGATGGGCTA CCGGCTGCGC TACAAACGTC TGGAAGAGGA AAACTATGAG 
ATACGCGAAT TCAGTCGCAC CGACGACCAC CACACCGTCA CTGACCTGCA CAAGGGCGCC 
ACCTACAGCT TCCACCTGAG CGCCAGAAAC CGCGCTGGAC TCGGAGAGGA GTACGTTAAA 
GACATCGGCA CTCCTGAGGA CGTGCCCAGC GGGTTTCCTC TTAACCTGCA GGTCGTAGGC 
TTGACTACCT CTACCACCCG ACTGACCTGG GAGCCTCCAG CGTTGTCTGA ACGTAACGGA 
CGGATCATAC ATTACATTGT GGTTTACCGA GACATCAACA GCAAACAAAA CAGCACCAAC 
CGAACCTCCG ACACACAGAT GACCATCCAA GGGCTCCGAC CTGACACCAC CTATGATATC 
CGTGTGCAGG CGTTCACTAG CAAGGGTGGA GGTCCCATAA GTCCCAGTAT CCAGAGCAGG 
ACCATGTCTA CGTCACCTGC ATTTGCCACA AGTTTTGGTG TCAAAGCTGT GATGAAAACC 
TCTGTCCTTC TCACCTGGGA GGTGCCAGAA AATTATAAAT CACAAGTGCC TTTTAAGATC 
CTCTATAACC AGCAGAGTGT GGAAGTCCAG GGGAATCTGA AGAGGAAGCT GATCACACGC 
CTGCAGCCGG ACACTGATTA CTCGTTTGTG CTGATGAGCC GGGGAAACAG CGCCGGTGGC 
CTACAACAGC AGGTCTCCAT CCGCACTGCA CCAGACCTGT TCAAAACTAA ACCTGTCCTG 
TACACGGCAG ACCAGACCAA CAATGGCAAA CTCACCATCA ACCTGCCCAA AGTGCCCACC 
TCTGCTCCTG TCAGGTGTTA TTACATTGTG GTCGTGCCCG TCTCTCAGAC ATCATCTCGT 
CAGTGGATTA ATCCCGATGA GATGGAGCTG GATGAGCTCC TGGAGTCTAG TGAGGGAGCT 
CATCTCAGGA GACGTCGACG GCACACAGAC ACCGTAAGAC CGTACATCGC CGCAAAGCTA 
TCCACGCTCC CAGAAACCTT CACTCTGGGC GATGAGCTAG ACTACAGCGG CTTCATCAAC 
CGGCCTCTTC CCAACAACCA ACACTTCCAG TGTTTTGTGA TGGCTGAACT CAAGGACCAA 
TACCCTGTTA CTGCCAATGA GAAGCAGCGG ACCTTCTCGG CTAGCCCATA CTCAGACCCC 
ATCATAGTGC AGGGTGAAAA CCAAATGCAG CGCAGTGTGG ACCAACCGGA GATGCTTTGG 
GTCATGGGCC CTGTGCTCGC TGTGGTGCTC ATCATCATCA TAGTCATCGC CATCCTGCTC 
TTCAAAAGCA AACAGGAGAG GAAGAGGGCT TCCCCCCTAC CAAAAGACGA TCATTCGGGT 
GGTGTGAAGG ATTGTCTTCT GGCCAGCTCA TCCGATCCTG TCGAAATGAG GAGACTCAAC 
TACCAGACAC CAGGAATGAG GGAACATCCT CCCATATCCG TCTCCGAACT GGCAGACCAC 
ATCGAGCGCC TCAAGGCCAA CGATGCCCTC CGGTTTTCCC AAGAGTATGA GTCTATTGAT 
CCAGGCCAGC AGTTCACATG GGAGAACTCC AACTTGGAGG TCAACAAGCC AAAGAACCGT 
TATGCCAACG TTATTGCTTA CGACCACTCG AGAGTTGTTC TCACCCCTGT GGACGGTGTA 
CCAGGGAGTG ACTACATTAA TTCCAACTAC ATTGATGGCT ACCGTAAGCA GAATGCCTAC 
ATTGCCACAC AAGGTCCTCT GCCTGAGACT CTGAGCGACT TCTGGAGGAT GATTTGGGAG 
CAACGAGCCA GTACCATCGT CATGATGACC AGACTGGAAG AGAAGTCCAG AGTCAAGTGT 
GACCAATACT GGCCCATTCG TGGCACAGAG ACTTATGGAA TGATTCAAGT GACCATGCTT 
GATGCTGTTG AGCTGGCAAC CTACAGTGTG CGCACCTTTG CCCTTTACAA GAATGGCTCT 
AGTGAGAAGC GCGAGGTCAG GCAGTTTCAG TTCATGGCTT GGCCGGACCA CGGAGTGCCT 
GAATATCCCA CCCCCATCCT AGCCTTCCTG CGCAGGGTCA AAGCCTGCAA CCCTCCTGAT 
GCTGGACCCA TGGTAGTGCA CTGCAGTGCT GGAGTTGGCC GTACAGGCTG CTTCATAGTC 
ATCGATGCGA TGTTGGAGCG CATGAAGCAC GAGAAGACCG TAGACATTTA TGGCCACGTC 
ACGTGCATGA GATCCCAGAG GAACTATATG GTGCAGACGG AGGACCAGTA CATCTTTATC 
CACGAGGCCC TTCTGGAGGC CGCCACCTGT GGAAACACAG AGGTCCCTGC CCGAAACCTC 
TACGCCCACA TCCAGAAGCT CACACAGCCA TCGGCTGGAG AGACCGTCAC AGCCATGGAG 
CTGGAGTTTA AGAGACTCGC CAACTCCAAA GCCCACACCT CAAGATTCAT CAGCGCCAAC 
CTGCCCTGCA ACAAGTTCAA GAACCGGCTG GTCAATATCA TGCCTTTCGA GTCCACACGG 
GTGTGCCTGC AGCCAATCCG AGGAGTGGAG GGGTCCGACT ACATCAATGC CAGCTGCATT 
GACGGATACA GACAGCAGAA GGCCTATATT GCCACTCAGG GTCCTCTAGC GGAGACCACC 
GAGGACTTCT GGAGGATGTT ATGGGAGCAC AATTCCACTA TTGTAGTGAT GCTGACCAAA 
CTCAGAGAGA TGGGACGGGA GAAGTGTCAT CAGTACTGGC CCGCCGAGCG CTCAGCCCGC 
TATCAGTACT TCGTAGTGGA TCCCATGGCC GAATACAACA TGCCCCAGTA CATCCTGCGA 
GAGTTCAAAG TCACAGATGC CAGGGACGGT CAATCCAGAA CCATTCGCCA GTTCCAGTTC 
ACTGATTGGC CAGAGCAGGG AGTGCCAAAA ACCGGTGAGG GGTTCATCGA TTTCATTGGG 
CAAGTTCATA AAACCAAGGA GCAGTTTGGG CAGGATGGAC CAATCACTGT GCACTGCAGT 
GCTGGAGTGG GAAGAACAGG TGTTTTCATC ACTCTCAGTA TTGTCCTGGA GAGGATGAGA 
TATGAGGGGG TGGTGGATCT GTTCCAGACG GTTAAAACCC TGCGCACACA AAGGCCTGCC 
ATGGTGCAGA CAGAGGACCA GTACCAGCTG TGCTACAGAG CCGCTCTGGA GTACCTGGGG 
AGCTTTGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298457.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296732.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X1, mRNA.

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CloneID	ODa47413
Clone ID Related Accession (Same CDS sequence)	XM_009298458.2 , XM_009298457.3 , XM_021481410.1 , XM_009298457.2 , XM_009298458.3
Accession Version	XM_009298457.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298457.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGTGGAA AATTGAAGAG TCTACGGAAT ATGAGGCTGT GATCGCTGGA 
CTTGCCTCTG AAACCTCTTA CTCAGTGACT GTGGCTGCGT ACACAACCAA AGGTGATGGA 
GCTCGCAGCA AAGCCAAAGT GGTCACCACC ACCGGAGCAG TGCCTGGCAA ACCCACTATG 
ATCATCAGCA CCACCGTTGG CAACACGGCC CTTATCCAGT GGCAGCCTCC TAAAGACATG 
GTGGGTGAGC TGATGGGCTA CCGGCTGCGC TACAAACGTC TGGAAGAGGA AAACTATGAG 
ATACGCGAAT TCAGTCGCAC CGACGACCAC CACACCGTCA CTGACCTGCA CAAGGGCGCC 
ACCTACAGCT TCCACCTGAG CGCCAGAAAC CGCGCTGGAC TCGGAGAGGA GTACGTTAAA 
GACATCGGCA CTCCTGAGGA CGTGCCCAGC GGGTTTCCTC TTAACCTGCA GGTCGTAGGC 
TTGACTACCT CTACCACCCG ACTGACCTGG GAGCCTCCAG CGTTGTCTGA ACGTAACGGA 
CGGATCATAC ATTACATTGT GGTTTACCGA GACATCAACA GCAAACAAAA CAGCACCAAC 
CGAACCTCCG ACACACAGAT GACCATCCAA GGGCTCCGAC CTGACACCAC CTATGATATC 
CGTGTGCAGG CGTTCACTAG CAAGGGTGGA GGTCCCATAA GTCCCAGTAT CCAGAGCAGG 
ACCATGTCTA CGTCACCTGC ATTTGCCACA AGTTTTGGTG TCAAAGCTGT GATGAAAACC 
TCTGTCCTTC TCACCTGGGA GGTGCCAGAA AATTATAAAT CACAAGTGCC TTTTAAGATC 
CTCTATAACC AGCAGAGTGT GGAAGTCCAG GGGAATCTGA AGAGGAAGCT GATCACACGC 
CTGCAGCCGG ACACTGATTA CTCGTTTGTG CTGATGAGCC GGGGAAACAG CGCCGGTGGC 
CTACAACAGC AGGTCTCCAT CCGCACTGCA CCAGACCTGT TCAAAACTAA ACCTGTCCTG 
TACACGGCAG ACCAGACCAA CAATGGCAAA CTCACCATCA ACCTGCCCAA AGTGCCCACC 
TCTGCTCCTG TCAGGTGTTA TTACATTGTG GTCGTGCCCG TCTCTCAGAC ATCATCTCGT 
CAGTGGATTA ATCCCGATGA GATGGAGCTG GATGAGCTCC TGGAGTCTAG TGAGGGAGCT 
CATCTCAGGA GACGTCGACG GCACACAGAC ACCGTAAGAC CGTACATCGC CGCAAAGCTA 
TCCACGCTCC CAGAAACCTT CACTCTGGGC GATGAGCTAG ACTACAGCGG CTTCATCAAC 
CGGCCTCTTC CCAACAACCA ACACTTCCAG TGTTTTGTGA TGGCTGAACT CAAGGACCAA 
TACCCTGTTA CTGCCAATGA GAAGCAGCGG ACCTTCTCGG CTAGCCCATA CTCAGACCCC 
ATCATAGTGC AGGGTGAAAA CCAAATGCAG CGCAGTGTGG ACCAACCGGA GATGCTTTGG 
GTCATGGGCC CTGTGCTCGC TGTGGTGCTC ATCATCATCA TAGTCATCGC CATCCTGCTC 
TTCAAAAGCA AACAGGAGAG GAAGAGGGCT TCCCCCCTAC CAAAAGACGA TCATTCGGGT 
GGTGTGAAGG ATTGTCTTCT GGCCAGCTCA TCCGATCCTG TCGAAATGAG GAGACTCAAC 
TACCAGACAC CAGGAATGAG GGAACATCCT CCCATATCCG TCTCCGAACT GGCAGACCAC 
ATCGAGCGCC TCAAGGCCAA CGATGCCCTC CGGTTTTCCC AAGAGTATGA GTCTATTGAT 
CCAGGCCAGC AGTTCACATG GGAGAACTCC AACTTGGAGG TCAACAAGCC AAAGAACCGT 
TATGCCAACG TTATTGCTTA CGACCACTCG AGAGTTGTTC TCACCCCTGT GGACGGTGTA 
CCAGGGAGTG ACTACATTAA TTCCAACTAC ATTGATGGCT ACCGTAAGCA GAATGCCTAC 
ATTGCCACAC AAGGTCCTCT GCCTGAGACT CTGAGCGACT TCTGGAGGAT GATTTGGGAG 
CAACGAGCCA GTACCATCGT CATGATGACC AGACTGGAAG AGAAGTCCAG AGTCAAGTGT 
GACCAATACT GGCCCATTCG TGGCACAGAG ACTTATGGAA TGATTCAAGT GACCATGCTT 
GATGCTGTTG AGCTGGCAAC CTACAGTGTG CGCACCTTTG CCCTTTACAA GAATGGCTCT 
AGTGAGAAGC GCGAGGTCAG GCAGTTTCAG TTCATGGCTT GGCCGGACCA CGGAGTGCCT 
GAATATCCCA CCCCCATCCT AGCCTTCCTG CGCAGGGTCA AAGCCTGCAA CCCTCCTGAT 
GCTGGACCCA TGGTAGTGCA CTGCAGTGCT GGAGTTGGCC GTACAGGCTG CTTCATAGTC 
ATCGATGCGA TGTTGGAGCG CATGAAGCAC GAGAAGACCG TAGACATTTA TGGCCACGTC 
ACGTGCATGA GATCCCAGAG GAACTATATG GTGCAGACGG AGGACCAGTA CATCTTTATC 
CACGAGGCCC TTCTGGAGGC CGCCACCTGT GGAAACACAG AGGTCCCTGC CCGAAACCTC 
TACGCCCACA TCCAGAAGCT CACACAGCCA TCGGCTGGAG AGACCGTCAC AGCCATGGAG 
CTGGAGTTTA AGAGACTCGC CAACTCCAAA GCCCACACCT CAAGATTCAT CAGCGCCAAC 
CTGCCCTGCA ACAAGTTCAA GAACCGGCTG GTCAATATCA TGCCTTTCGA GTCCACACGG 
GTGTGCCTGC AGCCAATCCG AGGAGTGGAG GGGTCCGACT ACATCAATGC CAGCTGCATT 
GACGGATACA GACAGCAGAA GGCCTATATT GCCACTCAGG GTCCTCTAGC GGAGACCACC 
GAGGACTTCT GGAGGATGTT ATGGGAGCAC AATTCCACTA TTGTAGTGAT GCTGACCAAA 
CTCAGAGAGA TGGGACGGGA GAAGTGTCAT CAGTACTGGC CCGCCGAGCG CTCAGCCCGC 
TATCAGTACT TCGTAGTGGA TCCCATGGCC GAATACAACA TGCCCCAGTA CATCCTGCGA 
GAGTTCAAAG TCACAGATGC CAGGGACGGT CAATCCAGAA CCATTCGCCA GTTCCAGTTC 
ACTGATTGGC CAGAGCAGGG AGTGCCAAAA ACCGGTGAGG GGTTCATCGA TTTCATTGGG 
CAAGTTCATA AAACCAAGGA GCAGTTTGGG CAGGATGGAC CAATCACTGT GCACTGCAGT 
GCTGGAGTGG GAAGAACAGG TGTTTTCATC ACTCTCAGTA TTGTCCTGGA GAGGATGAGA 
TATGAGGGGG TGGTGGATCT GTTCCAGACG GTTAAAACCC TGCGCACACA AAGGCCTGCC 
ATGGTGCAGA CAGAGGACCA GTACCAGCTG TGCTACAGAG CCGCTCTGGA GTACCTGGGG 
AGCTTTGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296732.1
CDS	426..6209
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVIREDPRGVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298457.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298457.2

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGTGGAA AATTGAAGAG TCTACGGAAT ATGAGGCTGT GATCGCTGGA 
CTTGCCTCTG AAACCTCTTA CTCAGTGACT GTGGCTGCGT ACACAACCAA AGGTGATGGA 
GCTCGCAGCA AAGCCAAAGT GGTCACCACC ACCGGAGCAG TGCCTGGCAA ACCCACTATG 
ATCATCAGCA CCACCGTTGG CAACACGGCC CTTATCCAGT GGCAGCCTCC TAAAGACATG 
GTGGGTGAGC TGATGGGCTA CCGGCTGCGC TACAAACGTC TGGAAGAGGA AAACTATGAG 
ATACGCGAAT TCAGTCGCAC CGACGACCAC CACACCGTCA CTGACCTGCA CAAGGGCGCC 
ACCTACAGCT TCCACCTGAG CGCCAGAAAC CGCGCTGGAC TCGGAGAGGA GTACGTTAAA 
GACATCGGCA CTCCTGAGGA CGTGCCCAGC GGGTTTCCTC TTAACCTGCA GGTCGTAGGC 
TTGACTACCT CTACCACCCG ACTGACCTGG GAGCCTCCAG CGTTGTCTGA ACGTAACGGA 
CGGATCATAC ATTACATTGT GGTTTACCGA GACATCAACA GCAAACAAAA CAGCACCAAC 
CGAACCTCCG ACACACAGAT GACCATCCAA GGGCTCCGAC CTGACACCAC CTATGATATC 
CGTGTGCAGG CGTTCACTAG CAAGGGTGGA GGTCCCATAA GTCCCAGTAT CCAGAGCAGG 
ACCATGTCTA CGTCACCTGC ATTTGCCACA AGTTTTGGTG TCAAAGCTGT GATGAAAACC 
TCTGTCCTTC TCACCTGGGA GGTGCCAGAA AATTATAAAT CACAAGTGCC TTTTAAGATC 
CTCTATAACC AGCAGAGTGT GGAAGTCCAG GGGAATCTGA AGAGGAAGCT GATCACACGC 
CTGCAGCCGG ACACTGATTA CTCGTTTGTG CTGATGAGCC GGGGAAACAG CGCCGGTGGC 
CTACAACAGC AGGTCTCCAT CCGCACTGCA CCAGACCTGT TCAAAACTAA ACCTGTCCTG 
TACACGGCAG ACCAGACCAA CAATGGCAAA CTCACCATCA ACCTGCCCAA AGTGCCCACC 
TCTGCTCCTG TCAGGTGTTA TTACATTGTG GTCGTGCCCG TCTCTCAGAC ATCATCTCGT 
CAGTGGATTA ATCCCGATGA GATGGAGCTG GATGAGCTCC TGGAGTCTAG TGAGGGAGCT 
CATCTCAGGA GACGTCGACG GCACACAGAC ACCGTAAGAC CGTACATCGC CGCAAAGCTA 
TCCACGCTCC CAGAAACCTT CACTCTGGGC GATGAGCTAG ACTACAGCGG CTTCATCAAC 
CGGCCTCTTC CCAACAACCA ACACTTCCAG TGTTTTGTGA TGGCTGAACT CAAGGACCAA 
TACCCTGTTA CTGCCAATGA GAAGCAGCGG ACCTTCTCGG CTAGCCCATA CTCAGACCCC 
ATCATAGTGC AGGGTGAAAA CCAAATGCAG CGCAGTGTGG ACCAACCGGA GATGCTTTGG 
GTCATGGGCC CTGTGCTCGC TGTGGTGCTC ATCATCATCA TAGTCATCGC CATCCTGCTC 
TTCAAAAGCA AACAGGAGAG GAAGAGGGCT TCCCCCCTAC CAAAAGACGA TCATTCGGGT 
GGTGTGAAGG ATTGTCTTCT GGCCAGCTCA TCCGATCCTG TCGAAATGAG GAGACTCAAC 
TACCAGACAC CAGGAATGAG GGAACATCCT CCCATATCCG TCTCCGAACT GGCAGACCAC 
ATCGAGCGCC TCAAGGCCAA CGATGCCCTC CGGTTTTCCC AAGAGTATGA GTCTATTGAT 
CCAGGCCAGC AGTTCACATG GGAGAACTCC AACTTGGAGG TCAACAAGCC AAAGAACCGT 
TATGCCAACG TTATTGCTTA CGACCACTCG AGAGTTGTTC TCACCCCTGT GGACGGTGTA 
CCAGGGAGTG ACTACATTAA TTCCAACTAC ATTGATGGCT ACCGTAAGCA GAATGCCTAC 
ATTGCCACAC AAGGTCCTCT GCCTGAGACT CTGAGCGACT TCTGGAGGAT GATTTGGGAG 
CAACGAGCCA GTACCATCGT CATGATGACC AGACTGGAAG AGAAGTCCAG AGTCAAGTGT 
GACCAATACT GGCCCATTCG TGGCACAGAG ACTTATGGAA TGATTCAAGT GACCATGCTT 
GATGCTGTTG AGCTGGCAAC CTACAGTGTG CGCACCTTTG CCCTTTACAA GAATGGCTCT 
AGTGAGAAGC GCGAGGTCAG GCAGTTTCAG TTCATGGCTT GGCCGGACCA CGGAGTGCCT 
GAATATCCCA CCCCCATCCT AGCCTTCCTG CGCAGGGTCA AAGCCTGCAA CCCTCCTGAT 
GCTGGACCCA TGGTAGTGCA CTGCAGTGCT GGAGTTGGCC GTACAGGCTG CTTCATAGTC 
ATCGATGCGA TGTTGGAGCG CATGAAGCAC GAGAAGACCG TAGACATTTA TGGCCACGTC 
ACGTGCATGA GATCCCAGAG GAACTATATG GTGCAGACGG AGGACCAGTA CATCTTTATC 
CACGAGGCCC TTCTGGAGGC CGCCACCTGT GGAAACACAG AGGTCCCTGC CCGAAACCTC 
TACGCCCACA TCCAGAAGCT CACACAGCCA TCGGCTGGAG AGACCGTCAC AGCCATGGAG 
CTGGAGTTTA AGAGACTCGC CAACTCCAAA GCCCACACCT CAAGATTCAT CAGCGCCAAC 
CTGCCCTGCA ACAAGTTCAA GAACCGGCTG GTCAATATCA TGCCTTTCGA GTCCACACGG 
GTGTGCCTGC AGCCAATCCG AGGAGTGGAG GGGTCCGACT ACATCAATGC CAGCTGCATT 
GACGGATACA GACAGCAGAA GGCCTATATT GCCACTCAGG GTCCTCTAGC GGAGACCACC 
GAGGACTTCT GGAGGATGTT ATGGGAGCAC AATTCCACTA TTGTAGTGAT GCTGACCAAA 
CTCAGAGAGA TGGGACGGGA GAAGTGTCAT CAGTACTGGC CCGCCGAGCG CTCAGCCCGC 
TATCAGTACT TCGTAGTGGA TCCCATGGCC GAATACAACA TGCCCCAGTA CATCCTGCGA 
GAGTTCAAAG TCACAGATGC CAGGGACGGT CAATCCAGAA CCATTCGCCA GTTCCAGTTC 
ACTGATTGGC CAGAGCAGGG AGTGCCAAAA ACCGGTGAGG GGTTCATCGA TTTCATTGGG 
CAAGTTCATA AAACCAAGGA GCAGTTTGGG CAGGATGGAC CAATCACTGT GCACTGCAGT 
GCTGGAGTGG GAAGAACAGG TGTTTTCATC ACTCTCAGTA TTGTCCTGGA GAGGATGAGA 
TATGAGGGGG TGGTGGATCT GTTCCAGACG GTTAAAACCC TGCGCACACA AAGGCCTGCC 
ATGGTGCAGA CAGAGGACCA GTACCAGCTG TGCTACAGAG CCGCTCTGGA GTACCTGGGG 
AGCTTTGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298458.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296733.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X3, mRNA.

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CloneID	ODa47413
Clone ID Related Accession (Same CDS sequence)	XM_009298458.2 , XM_009298457.3 , XM_021481410.1 , XM_009298457.2 , XM_009298458.3
Accession Version	XM_009298458.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298458.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGTGGAA AATTGAAGAG TCTACGGAAT ATGAGGCTGT GATCGCTGGA 
CTTGCCTCTG AAACCTCTTA CTCAGTGACT GTGGCTGCGT ACACAACCAA AGGTGATGGA 
GCTCGCAGCA AAGCCAAAGT GGTCACCACC ACCGGAGCAG TGCCTGGCAA ACCCACTATG 
ATCATCAGCA CCACCGTTGG CAACACGGCC CTTATCCAGT GGCAGCCTCC TAAAGACATG 
GTGGGTGAGC TGATGGGCTA CCGGCTGCGC TACAAACGTC TGGAAGAGGA AAACTATGAG 
ATACGCGAAT TCAGTCGCAC CGACGACCAC CACACCGTCA CTGACCTGCA CAAGGGCGCC 
ACCTACAGCT TCCACCTGAG CGCCAGAAAC CGCGCTGGAC TCGGAGAGGA GTACGTTAAA 
GACATCGGCA CTCCTGAGGA CGTGCCCAGC GGGTTTCCTC TTAACCTGCA GGTCGTAGGC 
TTGACTACCT CTACCACCCG ACTGACCTGG GAGCCTCCAG CGTTGTCTGA ACGTAACGGA 
CGGATCATAC ATTACATTGT GGTTTACCGA GACATCAACA GCAAACAAAA CAGCACCAAC 
CGAACCTCCG ACACACAGAT GACCATCCAA GGGCTCCGAC CTGACACCAC CTATGATATC 
CGTGTGCAGG CGTTCACTAG CAAGGGTGGA GGTCCCATAA GTCCCAGTAT CCAGAGCAGG 
ACCATGTCTA CGTCACCTGC ATTTGCCACA AGTTTTGGTG TCAAAGCTGT GATGAAAACC 
TCTGTCCTTC TCACCTGGGA GGTGCCAGAA AATTATAAAT CACAAGTGCC TTTTAAGATC 
CTCTATAACC AGCAGAGTGT GGAAGTCCAG GGGAATCTGA AGAGGAAGCT GATCACACGC 
CTGCAGCCGG ACACTGATTA CTCGTTTGTG CTGATGAGCC GGGGAAACAG CGCCGGTGGC 
CTACAACAGC AGGTCTCCAT CCGCACTGCA CCAGACCTGT TCAAAACTAA ACCTGTCCTG 
TACACGGCAG ACCAGACCAA CAATGGCAAA CTCACCATCA ACCTGCCCAA AGTGCCCACC 
TCTGCTCCTG TCAGGTGTTA TTACATTGTG GTCGTGCCCG TCTCTCAGAC ATCATCTCGT 
CAGTGGATTA ATCCCGATGA GATGGAGCTG GATGAGCTCC TGGAGTCTAG TGAGGGAGCT 
CATCTCAGGA GACGTCGACG GCACACAGAC ACCGTAAGAC CGTACATCGC CGCAAAGCTA 
TCCACGCTCC CAGAAACCTT CACTCTGGGC GATGAGCTAG ACTACAGCGG CTTCATCAAC 
CGGCCTCTTC CCAACAACCA ACACTTCCAG TGTTTTGTGA TGGCTGAACT CAAGGACCAA 
TACCCTGTTA CTGCCAATGA GAAGCAGCGG ACCTTCTCGG CTAGCCCATA CTCAGACCCC 
ATCATAGTGC AGGGTGAAAA CCAAATGCAG CGCAGTGTGG ACCAACCGGA GATGCTTTGG 
GTCATGGGCC CTGTGCTCGC TGTGGTGCTC ATCATCATCA TAGTCATCGC CATCCTGCTC 
TTCAAAAGCA AACAGGAGAG GAAGAGGGCT TCCCCCCTAC CAAAAGACGA TCATTCGGGT 
GGTGTGAAGG ATTGTCTTCT GGCCAGCTCA TCCGATCCTG TCGAAATGAG GAGACTCAAC 
TACCAGACAC CAGGAATGAG GGAACATCCT CCCATATCCG TCTCCGAACT GGCAGACCAC 
ATCGAGCGCC TCAAGGCCAA CGATGCCCTC CGGTTTTCCC AAGAGTATGA GTCTATTGAT 
CCAGGCCAGC AGTTCACATG GGAGAACTCC AACTTGGAGG TCAACAAGCC AAAGAACCGT 
TATGCCAACG TTATTGCTTA CGACCACTCG AGAGTTGTTC TCACCCCTGT GGACGGTGTA 
CCAGGGAGTG ACTACATTAA TTCCAACTAC ATTGATGGCT ACCGTAAGCA GAATGCCTAC 
ATTGCCACAC AAGGTCCTCT GCCTGAGACT CTGAGCGACT TCTGGAGGAT GATTTGGGAG 
CAACGAGCCA GTACCATCGT CATGATGACC AGACTGGAAG AGAAGTCCAG AGTCAAGTGT 
GACCAATACT GGCCCATTCG TGGCACAGAG ACTTATGGAA TGATTCAAGT GACCATGCTT 
GATGCTGTTG AGCTGGCAAC CTACAGTGTG CGCACCTTTG CCCTTTACAA GAATGGCTCT 
AGTGAGAAGC GCGAGGTCAG GCAGTTTCAG TTCATGGCTT GGCCGGACCA CGGAGTGCCT 
GAATATCCCA CCCCCATCCT AGCCTTCCTG CGCAGGGTCA AAGCCTGCAA CCCTCCTGAT 
GCTGGACCCA TGGTAGTGCA CTGCAGTGCT GGAGTTGGCC GTACAGGCTG CTTCATAGTC 
ATCGATGCGA TGTTGGAGCG CATGAAGCAC GAGAAGACCG TAGACATTTA TGGCCACGTC 
ACGTGCATGA GATCCCAGAG GAACTATATG GTGCAGACGG AGGACCAGTA CATCTTTATC 
CACGAGGCCC TTCTGGAGGC CGCCACCTGT GGAAACACAG AGGTCCCTGC CCGAAACCTC 
TACGCCCACA TCCAGAAGCT CACACAGCCA TCGGCTGGAG AGACCGTCAC AGCCATGGAG 
CTGGAGTTTA AGAGACTCGC CAACTCCAAA GCCCACACCT CAAGATTCAT CAGCGCCAAC 
CTGCCCTGCA ACAAGTTCAA GAACCGGCTG GTCAATATCA TGCCTTTCGA GTCCACACGG 
GTGTGCCTGC AGCCAATCCG AGGAGTGGAG GGGTCCGACT ACATCAATGC CAGCTGCATT 
GACGGATACA GACAGCAGAA GGCCTATATT GCCACTCAGG GTCCTCTAGC GGAGACCACC 
GAGGACTTCT GGAGGATGTT ATGGGAGCAC AATTCCACTA TTGTAGTGAT GCTGACCAAA 
CTCAGAGAGA TGGGACGGGA GAAGTGTCAT CAGTACTGGC CCGCCGAGCG CTCAGCCCGC 
TATCAGTACT TCGTAGTGGA TCCCATGGCC GAATACAACA TGCCCCAGTA CATCCTGCGA 
GAGTTCAAAG TCACAGATGC CAGGGACGGT CAATCCAGAA CCATTCGCCA GTTCCAGTTC 
ACTGATTGGC CAGAGCAGGG AGTGCCAAAA ACCGGTGAGG GGTTCATCGA TTTCATTGGG 
CAAGTTCATA AAACCAAGGA GCAGTTTGGG CAGGATGGAC CAATCACTGT GCACTGCAGT 
GCTGGAGTGG GAAGAACAGG TGTTTTCATC ACTCTCAGTA TTGTCCTGGA GAGGATGAGA 
TATGAGGGGG TGGTGGATCT GTTCCAGACG GTTAAAACCC TGCGCACACA AAGGCCTGCC 
ATGGTGCAGA CAGAGGACCA GTACCAGCTG TGCTACAGAG CCGCTCTGGA GTACCTGGGG 
AGCTTTGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296733.1
CDS	210..5993
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVIREDPRGVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298458.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298458.3

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGTGGAA AATTGAAGAG TCTACGGAAT ATGAGGCTGT GATCGCTGGA 
CTTGCCTCTG AAACCTCTTA CTCAGTGACT GTGGCTGCGT ACACAACCAA AGGTGATGGA 
GCTCGCAGCA AAGCCAAAGT GGTCACCACC ACCGGAGCAG TGCCTGGCAA ACCCACTATG 
ATCATCAGCA CCACCGTTGG CAACACGGCC CTTATCCAGT GGCAGCCTCC TAAAGACATG 
GTGGGTGAGC TGATGGGCTA CCGGCTGCGC TACAAACGTC TGGAAGAGGA AAACTATGAG 
ATACGCGAAT TCAGTCGCAC CGACGACCAC CACACCGTCA CTGACCTGCA CAAGGGCGCC 
ACCTACAGCT TCCACCTGAG CGCCAGAAAC CGCGCTGGAC TCGGAGAGGA GTACGTTAAA 
GACATCGGCA CTCCTGAGGA CGTGCCCAGC GGGTTTCCTC TTAACCTGCA GGTCGTAGGC 
TTGACTACCT CTACCACCCG ACTGACCTGG GAGCCTCCAG CGTTGTCTGA ACGTAACGGA 
CGGATCATAC ATTACATTGT GGTTTACCGA GACATCAACA GCAAACAAAA CAGCACCAAC 
CGAACCTCCG ACACACAGAT GACCATCCAA GGGCTCCGAC CTGACACCAC CTATGATATC 
CGTGTGCAGG CGTTCACTAG CAAGGGTGGA GGTCCCATAA GTCCCAGTAT CCAGAGCAGG 
ACCATGTCTA CGTCACCTGC ATTTGCCACA AGTTTTGGTG TCAAAGCTGT GATGAAAACC 
TCTGTCCTTC TCACCTGGGA GGTGCCAGAA AATTATAAAT CACAAGTGCC TTTTAAGATC 
CTCTATAACC AGCAGAGTGT GGAAGTCCAG GGGAATCTGA AGAGGAAGCT GATCACACGC 
CTGCAGCCGG ACACTGATTA CTCGTTTGTG CTGATGAGCC GGGGAAACAG CGCCGGTGGC 
CTACAACAGC AGGTCTCCAT CCGCACTGCA CCAGACCTGT TCAAAACTAA ACCTGTCCTG 
TACACGGCAG ACCAGACCAA CAATGGCAAA CTCACCATCA ACCTGCCCAA AGTGCCCACC 
TCTGCTCCTG TCAGGTGTTA TTACATTGTG GTCGTGCCCG TCTCTCAGAC ATCATCTCGT 
CAGTGGATTA ATCCCGATGA GATGGAGCTG GATGAGCTCC TGGAGTCTAG TGAGGGAGCT 
CATCTCAGGA GACGTCGACG GCACACAGAC ACCGTAAGAC CGTACATCGC CGCAAAGCTA 
TCCACGCTCC CAGAAACCTT CACTCTGGGC GATGAGCTAG ACTACAGCGG CTTCATCAAC 
CGGCCTCTTC CCAACAACCA ACACTTCCAG TGTTTTGTGA TGGCTGAACT CAAGGACCAA 
TACCCTGTTA CTGCCAATGA GAAGCAGCGG ACCTTCTCGG CTAGCCCATA CTCAGACCCC 
ATCATAGTGC AGGGTGAAAA CCAAATGCAG CGCAGTGTGG ACCAACCGGA GATGCTTTGG 
GTCATGGGCC CTGTGCTCGC TGTGGTGCTC ATCATCATCA TAGTCATCGC CATCCTGCTC 
TTCAAAAGCA AACAGGAGAG GAAGAGGGCT TCCCCCCTAC CAAAAGACGA TCATTCGGGT 
GGTGTGAAGG ATTGTCTTCT GGCCAGCTCA TCCGATCCTG TCGAAATGAG GAGACTCAAC 
TACCAGACAC CAGGAATGAG GGAACATCCT CCCATATCCG TCTCCGAACT GGCAGACCAC 
ATCGAGCGCC TCAAGGCCAA CGATGCCCTC CGGTTTTCCC AAGAGTATGA GTCTATTGAT 
CCAGGCCAGC AGTTCACATG GGAGAACTCC AACTTGGAGG TCAACAAGCC AAAGAACCGT 
TATGCCAACG TTATTGCTTA CGACCACTCG AGAGTTGTTC TCACCCCTGT GGACGGTGTA 
CCAGGGAGTG ACTACATTAA TTCCAACTAC ATTGATGGCT ACCGTAAGCA GAATGCCTAC 
ATTGCCACAC AAGGTCCTCT GCCTGAGACT CTGAGCGACT TCTGGAGGAT GATTTGGGAG 
CAACGAGCCA GTACCATCGT CATGATGACC AGACTGGAAG AGAAGTCCAG AGTCAAGTGT 
GACCAATACT GGCCCATTCG TGGCACAGAG ACTTATGGAA TGATTCAAGT GACCATGCTT 
GATGCTGTTG AGCTGGCAAC CTACAGTGTG CGCACCTTTG CCCTTTACAA GAATGGCTCT 
AGTGAGAAGC GCGAGGTCAG GCAGTTTCAG TTCATGGCTT GGCCGGACCA CGGAGTGCCT 
GAATATCCCA CCCCCATCCT AGCCTTCCTG CGCAGGGTCA AAGCCTGCAA CCCTCCTGAT 
GCTGGACCCA TGGTAGTGCA CTGCAGTGCT GGAGTTGGCC GTACAGGCTG CTTCATAGTC 
ATCGATGCGA TGTTGGAGCG CATGAAGCAC GAGAAGACCG TAGACATTTA TGGCCACGTC 
ACGTGCATGA GATCCCAGAG GAACTATATG GTGCAGACGG AGGACCAGTA CATCTTTATC 
CACGAGGCCC TTCTGGAGGC CGCCACCTGT GGAAACACAG AGGTCCCTGC CCGAAACCTC 
TACGCCCACA TCCAGAAGCT CACACAGCCA TCGGCTGGAG AGACCGTCAC AGCCATGGAG 
CTGGAGTTTA AGAGACTCGC CAACTCCAAA GCCCACACCT CAAGATTCAT CAGCGCCAAC 
CTGCCCTGCA ACAAGTTCAA GAACCGGCTG GTCAATATCA TGCCTTTCGA GTCCACACGG 
GTGTGCCTGC AGCCAATCCG AGGAGTGGAG GGGTCCGACT ACATCAATGC CAGCTGCATT 
GACGGATACA GACAGCAGAA GGCCTATATT GCCACTCAGG GTCCTCTAGC GGAGACCACC 
GAGGACTTCT GGAGGATGTT ATGGGAGCAC AATTCCACTA TTGTAGTGAT GCTGACCAAA 
CTCAGAGAGA TGGGACGGGA GAAGTGTCAT CAGTACTGGC CCGCCGAGCG CTCAGCCCGC 
TATCAGTACT TCGTAGTGGA TCCCATGGCC GAATACAACA TGCCCCAGTA CATCCTGCGA 
GAGTTCAAAG TCACAGATGC CAGGGACGGT CAATCCAGAA CCATTCGCCA GTTCCAGTTC 
ACTGATTGGC CAGAGCAGGG AGTGCCAAAA ACCGGTGAGG GGTTCATCGA TTTCATTGGG 
CAAGTTCATA AAACCAAGGA GCAGTTTGGG CAGGATGGAC CAATCACTGT GCACTGCAGT 
GCTGGAGTGG GAAGAACAGG TGTTTTCATC ACTCTCAGTA TTGTCCTGGA GAGGATGAGA 
TATGAGGGGG TGGTGGATCT GTTCCAGACG GTTAAAACCC TGCGCACACA AAGGCCTGCC 
ATGGTGCAGA CAGAGGACCA GTACCAGCTG TGCTACAGAG CCGCTCTGGA GTACCTGGGG 
AGCTTTGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298459.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296734.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X4, mRNA.

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CloneID	ODa47414
Clone ID Related Accession (Same CDS sequence)	XM_009298459.3 , XM_009298459.2
Accession Version	XM_009298459.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298459.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGTGGAA AATTGAAGAG TCTACGGAAT ATGAGGCTGT GATCGCTGGA 
CTTGCCTCTG AAACCTCTTA CTCAGTGACT GTGGCTGCGT ACACAACCAA AGGTGATGGA 
GCTCGCAGCA AAGCCAAAGT GGTCACCACC ACCGGAGCAG TGCCTGGCAA ACCCACTATG 
ATCATCAGCA CCACCGTTGG CAACACGGCC CTTATCCAGT GGCAGCCTCC TAAAGACATG 
GTGGGTGAGC TGATGGGCTA CCGGCTGCGC TACAAACGTC TGGAAGAGGA AAACTATGAG 
ATACGCGAAT TCAGTCGCAC CGACGACCAC CACACCGTCA CTGACCTGCA CAAGGGCGCC 
ACCTACAGCT TCCACCTGAG CGCCAGAAAC CGCGCTGGAC TCGGAGAGGA GTACGTTAAA 
GACATCGGCA CTCCTGAGGA CGTGCCCAGC GGGTTTCCTC TTAACCTGCA GGTCGTAGGC 
TTGACTACCT CTACCACCCG ACTGACCTGG GAGCCTCCAG CGTTGTCTGA ACGTAACGGA 
CGGATCATAC ATTACATTGT GGTTTACCGA GACATCAACA GCAAACAAAA CAGCACCAAC 
CGAACCTCCG ACACACAGAT GACCATCCAA GGGCTCCGAC CTGACACCAC CTATGATATC 
CGTGTGCAGG CGTTCACTAG CAAGGGTGGA GGTCCCATAA GTCCCAGTAT CCAGAGCAGG 
ACCATGTCTA CGTCACCTGC ATTTGCCACA AGTTTTGGTG TCAAAGCTGT GATGAAAACC 
TCTGTCCTTC TCACCTGGGA GGTGCCAGAA AATTATAAAT CACAAGTGCC TTTTAAGATC 
CTCTATAACC AGCAGAGTGT GGAAGTCCAG GGGAATCTGA AGAGGAAGCT GATCACACGC 
CTGCAGCCGG ACACTGATTA CTCGTTTGTG CTGATGAGCC GGGGAAACAG CGCCGGTGGC 
CTACAACAGC AGGTCTCCAT CCGCACTGCA CCAGACCTGT TCAAAACTAA ACCTGTCCTG 
TACACGGCAG ACCAGACCAA CAATGGCAAA CTCACCATCA ACCTGCCCAA AGTGCCCACC 
TCTGCTCCTG TCAGGTGTTA TTACATTGTG GTCGTGCCCG TCTCTCAGAC ATCATCTCGT 
CAGTGGATTA ATCCCGATGA GATGGAGCTG GATGAGCTCC TGGAGTCTAG TGAGGGAGCT 
CATCTCAGGA GACGTCGACG GCACACAGAC ACCGTAAGAC CGTACATCGC CGCAAAGCTA 
TCCACGCTCC CAGAAACCTT CACTCTGGGC GATGAGCTAG ACTACAGCGG CTTCATCAAC 
CGGCCTCTTC CCAACAACCA ACACTTCCAG TGTTTTGTGA TGGCTGAACT CAAGGACCAA 
TACCCTGTTA CTGCCAATGA GAAGCAGCGG ACCTTCTCGG CTAGCCCATA CTCAGACCCC 
ATCATAGTGC AGGGTGAAAA CCAAATGCAG CGCAGTGTGG ACCAACCGGA GATGCTTTGG 
GTCATGGGCC CTGTGCTCGC TGTGGTGCTC ATCATCATCA TAGTCATCGC CATCCTGCTC 
TTCAAAAGGA AGAGGGCTTC CCCCCTACCA AAAGACGATC ATTCGGGTGG TGTGAAGGAT 
TGTCTTCTGG CCAGCTCATC CGATCCTGTC GAAATGAGGA GACTCAACTA CCAGACACCA 
GGAATGAGGG AACATCCTCC CATATCCGTC TCCGAACTGG CAGACCACAT CGAGCGCCTC 
AAGGCCAACG ATGCCCTCCG GTTTTCCCAA GAGTATGAGT CTATTGATCC AGGCCAGCAG 
TTCACATGGG AGAACTCCAA CTTGGAGGTC AACAAGCCAA AGAACCGTTA TGCCAACGTT 
ATTGCTTACG ACCACTCGAG AGTTGTTCTC ACCCCTGTGG ACGGTGTACC AGGGAGTGAC 
TACATTAATT CCAACTACAT TGATGGCTAC CGTAAGCAGA ATGCCTACAT TGCCACACAA 
GGTCCTCTGC CTGAGACTCT GAGCGACTTC TGGAGGATGA TTTGGGAGCA ACGAGCCAGT 
ACCATCGTCA TGATGACCAG ACTGGAAGAG AAGTCCAGAG TCAAGTGTGA CCAATACTGG 
CCCATTCGTG GCACAGAGAC TTATGGAATG ATTCAAGTGA CCATGCTTGA TGCTGTTGAG 
CTGGCAACCT ACAGTGTGCG CACCTTTGCC CTTTACAAGA ATGGCTCTAG TGAGAAGCGC 
GAGGTCAGGC AGTTTCAGTT CATGGCTTGG CCGGACCACG GAGTGCCTGA ATATCCCACC 
CCCATCCTAG CCTTCCTGCG CAGGGTCAAA GCCTGCAACC CTCCTGATGC TGGACCCATG 
GTAGTGCACT GCAGTGCTGG AGTTGGCCGT ACAGGCTGCT TCATAGTCAT CGATGCGATG 
TTGGAGCGCA TGAAGCACGA GAAGACCGTA GACATTTATG GCCACGTCAC GTGCATGAGA 
TCCCAGAGGA ACTATATGGT GCAGACGGAG GACCAGTACA TCTTTATCCA CGAGGCCCTT 
CTGGAGGCCG CCACCTGTGG AAACACAGAG GTCCCTGCCC GAAACCTCTA CGCCCACATC 
CAGAAGCTCA CACAGCCATC GGCTGGAGAG ACCGTCACAG CCATGGAGCT GGAGTTTAAG 
AGACTCGCCA ACTCCAAAGC CCACACCTCA AGATTCATCA GCGCCAACCT GCCCTGCAAC 
AAGTTCAAGA ACCGGCTGGT CAATATCATG CCTTTCGAGT CCACACGGGT GTGCCTGCAG 
CCAATCCGAG GAGTGGAGGG GTCCGACTAC ATCAATGCCA GCTGCATTGA CGGATACAGA 
CAGCAGAAGG CCTATATTGC CACTCAGGGT CCTCTAGCGG AGACCACCGA GGACTTCTGG 
AGGATGTTAT GGGAGCACAA TTCCACTATT GTAGTGATGC TGACCAAACT CAGAGAGATG 
GGACGGGAGA AGTGTCATCA GTACTGGCCC GCCGAGCGCT CAGCCCGCTA TCAGTACTTC 
GTAGTGGATC CCATGGCCGA ATACAACATG CCCCAGTACA TCCTGCGAGA GTTCAAAGTC 
ACAGATGCCA GGGACGGTCA ATCCAGAACC ATTCGCCAGT TCCAGTTCAC TGATTGGCCA 
GAGCAGGGAG TGCCAAAAAC CGGTGAGGGG TTCATCGATT TCATTGGGCA AGTTCATAAA 
ACCAAGGAGC AGTTTGGGCA GGATGGACCA ATCACTGTGC ACTGCAGTGC TGGAGTGGGA 
AGAACAGGTG TTTTCATCAC TCTCAGTATT GTCCTGGAGA GGATGAGATA TGAGGGGGTG 
GTGGATCTGT TCCAGACGGT TAAAACCCTG CGCACACAAA GGCCTGCCAT GGTGCAGACA 
GAGGACCAGT ACCAGCTGTG CTACAGAGCC GCTCTGGAGT ACCTGGGGAG CTTTGATCAC 
TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296734.1
CDS	422..6193
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVIREDPRGVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKRKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298459.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298459.3

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGTGGAA AATTGAAGAG TCTACGGAAT ATGAGGCTGT GATCGCTGGA 
CTTGCCTCTG AAACCTCTTA CTCAGTGACT GTGGCTGCGT ACACAACCAA AGGTGATGGA 
GCTCGCAGCA AAGCCAAAGT GGTCACCACC ACCGGAGCAG TGCCTGGCAA ACCCACTATG 
ATCATCAGCA CCACCGTTGG CAACACGGCC CTTATCCAGT GGCAGCCTCC TAAAGACATG 
GTGGGTGAGC TGATGGGCTA CCGGCTGCGC TACAAACGTC TGGAAGAGGA AAACTATGAG 
ATACGCGAAT TCAGTCGCAC CGACGACCAC CACACCGTCA CTGACCTGCA CAAGGGCGCC 
ACCTACAGCT TCCACCTGAG CGCCAGAAAC CGCGCTGGAC TCGGAGAGGA GTACGTTAAA 
GACATCGGCA CTCCTGAGGA CGTGCCCAGC GGGTTTCCTC TTAACCTGCA GGTCGTAGGC 
TTGACTACCT CTACCACCCG ACTGACCTGG GAGCCTCCAG CGTTGTCTGA ACGTAACGGA 
CGGATCATAC ATTACATTGT GGTTTACCGA GACATCAACA GCAAACAAAA CAGCACCAAC 
CGAACCTCCG ACACACAGAT GACCATCCAA GGGCTCCGAC CTGACACCAC CTATGATATC 
CGTGTGCAGG CGTTCACTAG CAAGGGTGGA GGTCCCATAA GTCCCAGTAT CCAGAGCAGG 
ACCATGTCTA CGTCACCTGC ATTTGCCACA AGTTTTGGTG TCAAAGCTGT GATGAAAACC 
TCTGTCCTTC TCACCTGGGA GGTGCCAGAA AATTATAAAT CACAAGTGCC TTTTAAGATC 
CTCTATAACC AGCAGAGTGT GGAAGTCCAG GGGAATCTGA AGAGGAAGCT GATCACACGC 
CTGCAGCCGG ACACTGATTA CTCGTTTGTG CTGATGAGCC GGGGAAACAG CGCCGGTGGC 
CTACAACAGC AGGTCTCCAT CCGCACTGCA CCAGACCTGT TCAAAACTAA ACCTGTCCTG 
TACACGGCAG ACCAGACCAA CAATGGCAAA CTCACCATCA ACCTGCCCAA AGTGCCCACC 
TCTGCTCCTG TCAGGTGTTA TTACATTGTG GTCGTGCCCG TCTCTCAGAC ATCATCTCGT 
CAGTGGATTA ATCCCGATGA GATGGAGCTG GATGAGCTCC TGGAGTCTAG TGAGGGAGCT 
CATCTCAGGA GACGTCGACG GCACACAGAC ACCGTAAGAC CGTACATCGC CGCAAAGCTA 
TCCACGCTCC CAGAAACCTT CACTCTGGGC GATGAGCTAG ACTACAGCGG CTTCATCAAC 
CGGCCTCTTC CCAACAACCA ACACTTCCAG TGTTTTGTGA TGGCTGAACT CAAGGACCAA 
TACCCTGTTA CTGCCAATGA GAAGCAGCGG ACCTTCTCGG CTAGCCCATA CTCAGACCCC 
ATCATAGTGC AGGGTGAAAA CCAAATGCAG CGCAGTGTGG ACCAACCGGA GATGCTTTGG 
GTCATGGGCC CTGTGCTCGC TGTGGTGCTC ATCATCATCA TAGTCATCGC CATCCTGCTC 
TTCAAAAGGA AGAGGGCTTC CCCCCTACCA AAAGACGATC ATTCGGGTGG TGTGAAGGAT 
TGTCTTCTGG CCAGCTCATC CGATCCTGTC GAAATGAGGA GACTCAACTA CCAGACACCA 
GGAATGAGGG AACATCCTCC CATATCCGTC TCCGAACTGG CAGACCACAT CGAGCGCCTC 
AAGGCCAACG ATGCCCTCCG GTTTTCCCAA GAGTATGAGT CTATTGATCC AGGCCAGCAG 
TTCACATGGG AGAACTCCAA CTTGGAGGTC AACAAGCCAA AGAACCGTTA TGCCAACGTT 
ATTGCTTACG ACCACTCGAG AGTTGTTCTC ACCCCTGTGG ACGGTGTACC AGGGAGTGAC 
TACATTAATT CCAACTACAT TGATGGCTAC CGTAAGCAGA ATGCCTACAT TGCCACACAA 
GGTCCTCTGC CTGAGACTCT GAGCGACTTC TGGAGGATGA TTTGGGAGCA ACGAGCCAGT 
ACCATCGTCA TGATGACCAG ACTGGAAGAG AAGTCCAGAG TCAAGTGTGA CCAATACTGG 
CCCATTCGTG GCACAGAGAC TTATGGAATG ATTCAAGTGA CCATGCTTGA TGCTGTTGAG 
CTGGCAACCT ACAGTGTGCG CACCTTTGCC CTTTACAAGA ATGGCTCTAG TGAGAAGCGC 
GAGGTCAGGC AGTTTCAGTT CATGGCTTGG CCGGACCACG GAGTGCCTGA ATATCCCACC 
CCCATCCTAG CCTTCCTGCG CAGGGTCAAA GCCTGCAACC CTCCTGATGC TGGACCCATG 
GTAGTGCACT GCAGTGCTGG AGTTGGCCGT ACAGGCTGCT TCATAGTCAT CGATGCGATG 
TTGGAGCGCA TGAAGCACGA GAAGACCGTA GACATTTATG GCCACGTCAC GTGCATGAGA 
TCCCAGAGGA ACTATATGGT GCAGACGGAG GACCAGTACA TCTTTATCCA CGAGGCCCTT 
CTGGAGGCCG CCACCTGTGG AAACACAGAG GTCCCTGCCC GAAACCTCTA CGCCCACATC 
CAGAAGCTCA CACAGCCATC GGCTGGAGAG ACCGTCACAG CCATGGAGCT GGAGTTTAAG 
AGACTCGCCA ACTCCAAAGC CCACACCTCA AGATTCATCA GCGCCAACCT GCCCTGCAAC 
AAGTTCAAGA ACCGGCTGGT CAATATCATG CCTTTCGAGT CCACACGGGT GTGCCTGCAG 
CCAATCCGAG GAGTGGAGGG GTCCGACTAC ATCAATGCCA GCTGCATTGA CGGATACAGA 
CAGCAGAAGG CCTATATTGC CACTCAGGGT CCTCTAGCGG AGACCACCGA GGACTTCTGG 
AGGATGTTAT GGGAGCACAA TTCCACTATT GTAGTGATGC TGACCAAACT CAGAGAGATG 
GGACGGGAGA AGTGTCATCA GTACTGGCCC GCCGAGCGCT CAGCCCGCTA TCAGTACTTC 
GTAGTGGATC CCATGGCCGA ATACAACATG CCCCAGTACA TCCTGCGAGA GTTCAAAGTC 
ACAGATGCCA GGGACGGTCA ATCCAGAACC ATTCGCCAGT TCCAGTTCAC TGATTGGCCA 
GAGCAGGGAG TGCCAAAAAC CGGTGAGGGG TTCATCGATT TCATTGGGCA AGTTCATAAA 
ACCAAGGAGC AGTTTGGGCA GGATGGACCA ATCACTGTGC ACTGCAGTGC TGGAGTGGGA 
AGAACAGGTG TTTTCATCAC TCTCAGTATT GTCCTGGAGA GGATGAGATA TGAGGGGGTG 
GTGGATCTGT TCCAGACGGT TAAAACCCTG CGCACACAAA GGCCTGCCAT GGTGCAGACA 
GAGGACCAGT ACCAGCTGTG CTACAGAGCC GCTCTGGAGT ACCTGGGGAG CTTTGATCAC 
TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298459.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296734.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X3, mRNA.

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CloneID	ODa47414
Clone ID Related Accession (Same CDS sequence)	XM_009298459.3 , XM_009298459.2
Accession Version	XM_009298459.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298459.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGTGGAA AATTGAAGAG TCTACGGAAT ATGAGGCTGT GATCGCTGGA 
CTTGCCTCTG AAACCTCTTA CTCAGTGACT GTGGCTGCGT ACACAACCAA AGGTGATGGA 
GCTCGCAGCA AAGCCAAAGT GGTCACCACC ACCGGAGCAG TGCCTGGCAA ACCCACTATG 
ATCATCAGCA CCACCGTTGG CAACACGGCC CTTATCCAGT GGCAGCCTCC TAAAGACATG 
GTGGGTGAGC TGATGGGCTA CCGGCTGCGC TACAAACGTC TGGAAGAGGA AAACTATGAG 
ATACGCGAAT TCAGTCGCAC CGACGACCAC CACACCGTCA CTGACCTGCA CAAGGGCGCC 
ACCTACAGCT TCCACCTGAG CGCCAGAAAC CGCGCTGGAC TCGGAGAGGA GTACGTTAAA 
GACATCGGCA CTCCTGAGGA CGTGCCCAGC GGGTTTCCTC TTAACCTGCA GGTCGTAGGC 
TTGACTACCT CTACCACCCG ACTGACCTGG GAGCCTCCAG CGTTGTCTGA ACGTAACGGA 
CGGATCATAC ATTACATTGT GGTTTACCGA GACATCAACA GCAAACAAAA CAGCACCAAC 
CGAACCTCCG ACACACAGAT GACCATCCAA GGGCTCCGAC CTGACACCAC CTATGATATC 
CGTGTGCAGG CGTTCACTAG CAAGGGTGGA GGTCCCATAA GTCCCAGTAT CCAGAGCAGG 
ACCATGTCTA CGTCACCTGC ATTTGCCACA AGTTTTGGTG TCAAAGCTGT GATGAAAACC 
TCTGTCCTTC TCACCTGGGA GGTGCCAGAA AATTATAAAT CACAAGTGCC TTTTAAGATC 
CTCTATAACC AGCAGAGTGT GGAAGTCCAG GGGAATCTGA AGAGGAAGCT GATCACACGC 
CTGCAGCCGG ACACTGATTA CTCGTTTGTG CTGATGAGCC GGGGAAACAG CGCCGGTGGC 
CTACAACAGC AGGTCTCCAT CCGCACTGCA CCAGACCTGT TCAAAACTAA ACCTGTCCTG 
TACACGGCAG ACCAGACCAA CAATGGCAAA CTCACCATCA ACCTGCCCAA AGTGCCCACC 
TCTGCTCCTG TCAGGTGTTA TTACATTGTG GTCGTGCCCG TCTCTCAGAC ATCATCTCGT 
CAGTGGATTA ATCCCGATGA GATGGAGCTG GATGAGCTCC TGGAGTCTAG TGAGGGAGCT 
CATCTCAGGA GACGTCGACG GCACACAGAC ACCGTAAGAC CGTACATCGC CGCAAAGCTA 
TCCACGCTCC CAGAAACCTT CACTCTGGGC GATGAGCTAG ACTACAGCGG CTTCATCAAC 
CGGCCTCTTC CCAACAACCA ACACTTCCAG TGTTTTGTGA TGGCTGAACT CAAGGACCAA 
TACCCTGTTA CTGCCAATGA GAAGCAGCGG ACCTTCTCGG CTAGCCCATA CTCAGACCCC 
ATCATAGTGC AGGGTGAAAA CCAAATGCAG CGCAGTGTGG ACCAACCGGA GATGCTTTGG 
GTCATGGGCC CTGTGCTCGC TGTGGTGCTC ATCATCATCA TAGTCATCGC CATCCTGCTC 
TTCAAAAGGA AGAGGGCTTC CCCCCTACCA AAAGACGATC ATTCGGGTGG TGTGAAGGAT 
TGTCTTCTGG CCAGCTCATC CGATCCTGTC GAAATGAGGA GACTCAACTA CCAGACACCA 
GGAATGAGGG AACATCCTCC CATATCCGTC TCCGAACTGG CAGACCACAT CGAGCGCCTC 
AAGGCCAACG ATGCCCTCCG GTTTTCCCAA GAGTATGAGT CTATTGATCC AGGCCAGCAG 
TTCACATGGG AGAACTCCAA CTTGGAGGTC AACAAGCCAA AGAACCGTTA TGCCAACGTT 
ATTGCTTACG ACCACTCGAG AGTTGTTCTC ACCCCTGTGG ACGGTGTACC AGGGAGTGAC 
TACATTAATT CCAACTACAT TGATGGCTAC CGTAAGCAGA ATGCCTACAT TGCCACACAA 
GGTCCTCTGC CTGAGACTCT GAGCGACTTC TGGAGGATGA TTTGGGAGCA ACGAGCCAGT 
ACCATCGTCA TGATGACCAG ACTGGAAGAG AAGTCCAGAG TCAAGTGTGA CCAATACTGG 
CCCATTCGTG GCACAGAGAC TTATGGAATG ATTCAAGTGA CCATGCTTGA TGCTGTTGAG 
CTGGCAACCT ACAGTGTGCG CACCTTTGCC CTTTACAAGA ATGGCTCTAG TGAGAAGCGC 
GAGGTCAGGC AGTTTCAGTT CATGGCTTGG CCGGACCACG GAGTGCCTGA ATATCCCACC 
CCCATCCTAG CCTTCCTGCG CAGGGTCAAA GCCTGCAACC CTCCTGATGC TGGACCCATG 
GTAGTGCACT GCAGTGCTGG AGTTGGCCGT ACAGGCTGCT TCATAGTCAT CGATGCGATG 
TTGGAGCGCA TGAAGCACGA GAAGACCGTA GACATTTATG GCCACGTCAC GTGCATGAGA 
TCCCAGAGGA ACTATATGGT GCAGACGGAG GACCAGTACA TCTTTATCCA CGAGGCCCTT 
CTGGAGGCCG CCACCTGTGG AAACACAGAG GTCCCTGCCC GAAACCTCTA CGCCCACATC 
CAGAAGCTCA CACAGCCATC GGCTGGAGAG ACCGTCACAG CCATGGAGCT GGAGTTTAAG 
AGACTCGCCA ACTCCAAAGC CCACACCTCA AGATTCATCA GCGCCAACCT GCCCTGCAAC 
AAGTTCAAGA ACCGGCTGGT CAATATCATG CCTTTCGAGT CCACACGGGT GTGCCTGCAG 
CCAATCCGAG GAGTGGAGGG GTCCGACTAC ATCAATGCCA GCTGCATTGA CGGATACAGA 
CAGCAGAAGG CCTATATTGC CACTCAGGGT CCTCTAGCGG AGACCACCGA GGACTTCTGG 
AGGATGTTAT GGGAGCACAA TTCCACTATT GTAGTGATGC TGACCAAACT CAGAGAGATG 
GGACGGGAGA AGTGTCATCA GTACTGGCCC GCCGAGCGCT CAGCCCGCTA TCAGTACTTC 
GTAGTGGATC CCATGGCCGA ATACAACATG CCCCAGTACA TCCTGCGAGA GTTCAAAGTC 
ACAGATGCCA GGGACGGTCA ATCCAGAACC ATTCGCCAGT TCCAGTTCAC TGATTGGCCA 
GAGCAGGGAG TGCCAAAAAC CGGTGAGGGG TTCATCGATT TCATTGGGCA AGTTCATAAA 
ACCAAGGAGC AGTTTGGGCA GGATGGACCA ATCACTGTGC ACTGCAGTGC TGGAGTGGGA 
AGAACAGGTG TTTTCATCAC TCTCAGTATT GTCCTGGAGA GGATGAGATA TGAGGGGGTG 
GTGGATCTGT TCCAGACGGT TAAAACCCTG CGCACACAAA GGCCTGCCAT GGTGCAGACA 
GAGGACCAGT ACCAGCTGTG CTACAGAGCC GCTCTGGAGT ACCTGGGGAG CTTTGATCAC 
TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296734.1
CDS	426..6197
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVIREDPRGVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKRKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298459.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298459.2

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGTGGAA AATTGAAGAG TCTACGGAAT ATGAGGCTGT GATCGCTGGA 
CTTGCCTCTG AAACCTCTTA CTCAGTGACT GTGGCTGCGT ACACAACCAA AGGTGATGGA 
GCTCGCAGCA AAGCCAAAGT GGTCACCACC ACCGGAGCAG TGCCTGGCAA ACCCACTATG 
ATCATCAGCA CCACCGTTGG CAACACGGCC CTTATCCAGT GGCAGCCTCC TAAAGACATG 
GTGGGTGAGC TGATGGGCTA CCGGCTGCGC TACAAACGTC TGGAAGAGGA AAACTATGAG 
ATACGCGAAT TCAGTCGCAC CGACGACCAC CACACCGTCA CTGACCTGCA CAAGGGCGCC 
ACCTACAGCT TCCACCTGAG CGCCAGAAAC CGCGCTGGAC TCGGAGAGGA GTACGTTAAA 
GACATCGGCA CTCCTGAGGA CGTGCCCAGC GGGTTTCCTC TTAACCTGCA GGTCGTAGGC 
TTGACTACCT CTACCACCCG ACTGACCTGG GAGCCTCCAG CGTTGTCTGA ACGTAACGGA 
CGGATCATAC ATTACATTGT GGTTTACCGA GACATCAACA GCAAACAAAA CAGCACCAAC 
CGAACCTCCG ACACACAGAT GACCATCCAA GGGCTCCGAC CTGACACCAC CTATGATATC 
CGTGTGCAGG CGTTCACTAG CAAGGGTGGA GGTCCCATAA GTCCCAGTAT CCAGAGCAGG 
ACCATGTCTA CGTCACCTGC ATTTGCCACA AGTTTTGGTG TCAAAGCTGT GATGAAAACC 
TCTGTCCTTC TCACCTGGGA GGTGCCAGAA AATTATAAAT CACAAGTGCC TTTTAAGATC 
CTCTATAACC AGCAGAGTGT GGAAGTCCAG GGGAATCTGA AGAGGAAGCT GATCACACGC 
CTGCAGCCGG ACACTGATTA CTCGTTTGTG CTGATGAGCC GGGGAAACAG CGCCGGTGGC 
CTACAACAGC AGGTCTCCAT CCGCACTGCA CCAGACCTGT TCAAAACTAA ACCTGTCCTG 
TACACGGCAG ACCAGACCAA CAATGGCAAA CTCACCATCA ACCTGCCCAA AGTGCCCACC 
TCTGCTCCTG TCAGGTGTTA TTACATTGTG GTCGTGCCCG TCTCTCAGAC ATCATCTCGT 
CAGTGGATTA ATCCCGATGA GATGGAGCTG GATGAGCTCC TGGAGTCTAG TGAGGGAGCT 
CATCTCAGGA GACGTCGACG GCACACAGAC ACCGTAAGAC CGTACATCGC CGCAAAGCTA 
TCCACGCTCC CAGAAACCTT CACTCTGGGC GATGAGCTAG ACTACAGCGG CTTCATCAAC 
CGGCCTCTTC CCAACAACCA ACACTTCCAG TGTTTTGTGA TGGCTGAACT CAAGGACCAA 
TACCCTGTTA CTGCCAATGA GAAGCAGCGG ACCTTCTCGG CTAGCCCATA CTCAGACCCC 
ATCATAGTGC AGGGTGAAAA CCAAATGCAG CGCAGTGTGG ACCAACCGGA GATGCTTTGG 
GTCATGGGCC CTGTGCTCGC TGTGGTGCTC ATCATCATCA TAGTCATCGC CATCCTGCTC 
TTCAAAAGGA AGAGGGCTTC CCCCCTACCA AAAGACGATC ATTCGGGTGG TGTGAAGGAT 
TGTCTTCTGG CCAGCTCATC CGATCCTGTC GAAATGAGGA GACTCAACTA CCAGACACCA 
GGAATGAGGG AACATCCTCC CATATCCGTC TCCGAACTGG CAGACCACAT CGAGCGCCTC 
AAGGCCAACG ATGCCCTCCG GTTTTCCCAA GAGTATGAGT CTATTGATCC AGGCCAGCAG 
TTCACATGGG AGAACTCCAA CTTGGAGGTC AACAAGCCAA AGAACCGTTA TGCCAACGTT 
ATTGCTTACG ACCACTCGAG AGTTGTTCTC ACCCCTGTGG ACGGTGTACC AGGGAGTGAC 
TACATTAATT CCAACTACAT TGATGGCTAC CGTAAGCAGA ATGCCTACAT TGCCACACAA 
GGTCCTCTGC CTGAGACTCT GAGCGACTTC TGGAGGATGA TTTGGGAGCA ACGAGCCAGT 
ACCATCGTCA TGATGACCAG ACTGGAAGAG AAGTCCAGAG TCAAGTGTGA CCAATACTGG 
CCCATTCGTG GCACAGAGAC TTATGGAATG ATTCAAGTGA CCATGCTTGA TGCTGTTGAG 
CTGGCAACCT ACAGTGTGCG CACCTTTGCC CTTTACAAGA ATGGCTCTAG TGAGAAGCGC 
GAGGTCAGGC AGTTTCAGTT CATGGCTTGG CCGGACCACG GAGTGCCTGA ATATCCCACC 
CCCATCCTAG CCTTCCTGCG CAGGGTCAAA GCCTGCAACC CTCCTGATGC TGGACCCATG 
GTAGTGCACT GCAGTGCTGG AGTTGGCCGT ACAGGCTGCT TCATAGTCAT CGATGCGATG 
TTGGAGCGCA TGAAGCACGA GAAGACCGTA GACATTTATG GCCACGTCAC GTGCATGAGA 
TCCCAGAGGA ACTATATGGT GCAGACGGAG GACCAGTACA TCTTTATCCA CGAGGCCCTT 
CTGGAGGCCG CCACCTGTGG AAACACAGAG GTCCCTGCCC GAAACCTCTA CGCCCACATC 
CAGAAGCTCA CACAGCCATC GGCTGGAGAG ACCGTCACAG CCATGGAGCT GGAGTTTAAG 
AGACTCGCCA ACTCCAAAGC CCACACCTCA AGATTCATCA GCGCCAACCT GCCCTGCAAC 
AAGTTCAAGA ACCGGCTGGT CAATATCATG CCTTTCGAGT CCACACGGGT GTGCCTGCAG 
CCAATCCGAG GAGTGGAGGG GTCCGACTAC ATCAATGCCA GCTGCATTGA CGGATACAGA 
CAGCAGAAGG CCTATATTGC CACTCAGGGT CCTCTAGCGG AGACCACCGA GGACTTCTGG 
AGGATGTTAT GGGAGCACAA TTCCACTATT GTAGTGATGC TGACCAAACT CAGAGAGATG 
GGACGGGAGA AGTGTCATCA GTACTGGCCC GCCGAGCGCT CAGCCCGCTA TCAGTACTTC 
GTAGTGGATC CCATGGCCGA ATACAACATG CCCCAGTACA TCCTGCGAGA GTTCAAAGTC 
ACAGATGCCA GGGACGGTCA ATCCAGAACC ATTCGCCAGT TCCAGTTCAC TGATTGGCCA 
GAGCAGGGAG TGCCAAAAAC CGGTGAGGGG TTCATCGATT TCATTGGGCA AGTTCATAAA 
ACCAAGGAGC AGTTTGGGCA GGATGGACCA ATCACTGTGC ACTGCAGTGC TGGAGTGGGA 
AGAACAGGTG TTTTCATCAC TCTCAGTATT GTCCTGGAGA GGATGAGATA TGAGGGGGTG 
GTGGATCTGT TCCAGACGGT TAAAACCCTG CGCACACAAA GGCCTGCCAT GGTGCAGACA 
GAGGACCAGT ACCAGCTGTG CTACAGAGCC GCTCTGGAGT ACCTGGGGAG CTTTGATCAC 
TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298460.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296735.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X4, mRNA.

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CloneID	ODa47415
Clone ID Related Accession (Same CDS sequence)	XM_009298460.2 , XM_009298460.3
Accession Version	XM_009298460.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298460.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG ACCCTCGAGG AGTACGACGA 
GTTCCACCCC GTTTCTCCAT CCCACCTACC AATCACGAGG TGATGCCAGG GGGCAGTGTA 
AACCTCACCT GCGTGGCAGT AGGTGCCCCG ATGCCCTACG TCAAATGGAT GACTGGTGAG 
GTGGAGCTCA CCAAGGATGA GGAGATGCCT GTGGGACGCA ACGTCTTGGA GCTCACCAAC 
ATCCGTCAGT CGACCAACTA CACCTGCGTG GCTATATCAT CCCTAGGTAT GATCGAGGCA 
ACGGCACAAG TGTCTGTCAA AGCTCTCCCA AAGCCTCCTA CATCCCTGAC TGTTACGGAA 
ACCACAGCTA CGAGTGTTAC ACTCACATGG GACTCTGGAA ACCCTGAACC TGTGTCTTAC 
TACATAATTC AATACCGGGC CAAGACTTCA GACACCAACT TCCAAGAAGT GGACGGTGTG 
GCCACCACAC GTTACAGCAT CGGCGGTCTC AGCCCATATT CAGAGTATGA GTTTCGAGTA 
ATGGCAGTCA ACAACATTGG TCGTGGGCCA CCTAGTGAAC CCGTTGAAAC ACGAACAGGT 
GAACAGGCAC CTTCCTCCCC ACCGCTGCAT GTTCAAGCAC GAATGCTGAG TGCCAGCACC 
ATGTTGGTTC AGTGGGAACC ACCAGAGGAA CCCAATGGTC AGATCAGAGG CTATCGGATC 
TACTACACAT CTGACCTGGA TGCACCTCTT AGCGCCTGGC AGAAACACAA CACAGACGAT 
AGCCGTCTCA CCACTATCTC CAGCCTGACC ACCGGCATCA CCTACAGCCT ACGTGTGCTG 
GGCTTCACAT CTGTAGGGGA TGGACCACCA TCTGATGTGC TACAGGTCAA GACACAGCAA 
GGAGTTCCTG CTCAGCCATC CAGCTTCGAA GCTGAAGCAG AGCTAGATAC AAGGATCCTA 
CTAACTTGGC TGTGGCCCGT GCAGGACCCC ATCATCAAAT ACGAGCTGCA ATATTGGGAG 
GCTGACTCAG ACAACAAGAT CCATGTCACC TTTGACCCAG CTGGCTCGTA CGCTGTTGAA 
GGTCTGAAAC CAGACACTTT GTACAAATTC TCTCTGGCAG CCCGCTCTGA AATGGGACAA 
GGAGTCTTCA CTCACCCCAT TGAAGCCAGG ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT 
CAAGACGTGC ACCTGCTCAG TCTGAGCTCC ACCAGCATCA AGGTGAGTTG GGTGGCACCA 
CCCGCCGCTA GCCGCCACGG CGCAATAGTG CGCTACACAG TCAGCTACCA GGCAGTGAAT 
GGAGAAGACA CAGAGCGCCA TGAGGTGCCG GACATTGGAG CGGATGCCTC CAGCTGTGTG 
TTGGAAGGTC TAGAGAAGTG GACGGAGTAT CAGGTGTGGG TGAGAGCGCA CACTGATGTG 
GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT ATCCGAACCA ATGAAGACGT GCCAGGAGCA 
CCGCCTCGGA AATTGGAAGT GGAGGCTTTG AACTCCACCG CTATCCGTGT GACATGGAAA 
CCTCCTCTTT CTGGAAAGCA GCATGGCCAG ATCCGGGGAT ATCAGGTCAT ATACTCCCGG 
TTGGAGAACG GGGAACCACG GGGACATCCG AATATAATGG ATGTCGCTCT GCCAGAAGCA 
CAGTGGAAAA TTGAAGAGTC TACGGAATAT GAGGCTGTGA TCGCTGGACT TGCCTCTGAA 
ACCTCTTACT CAGTGACTGT GGCTGCGTAC ACAACCAAAG GTGATGGAGC TCGCAGCAAA 
GCCAAAGTGG TCACCACCAC CGGAGCAGTG CCTGGCAAAC CCACTATGAT CATCAGCACC 
ACCGTTGGCA ACACGGCCCT TATCCAGTGG CAGCCTCCTA AAGACATGGT GGGTGAGCTG 
ATGGGCTACC GGCTGCGCTA CAAACGTCTG GAAGAGGAAA ACTATGAGAT ACGCGAATTC 
AGTCGCACCG ACGACCACCA CACCGTCACT GACCTGCACA AGGGCGCCAC CTACAGCTTC 
CACCTGAGCG CCAGAAACCG CGCTGGACTC GGAGAGGAGT ACGTTAAAGA CATCGGCACT 
CCTGAGGACG TGCCCAGCGG GTTTCCTCTT AACCTGCAGG TCGTAGGCTT GACTACCTCT 
ACCACCCGAC TGACCTGGGA GCCTCCAGCG TTGTCTGAAC GTAACGGACG GATCATACAT 
TACATTGTGG TTTACCGAGA CATCAACAGC AAACAAAACA GCACCAACCG AACCTCCGAC 
ACACAGATGA CCATCCAAGG GCTCCGACCT GACACCACCT ATGATATCCG TGTGCAGGCG 
TTCACTAGCA AGGGTGGAGG TCCCATAAGT CCCAGTATCC AGAGCAGGAC CATGTCTACG 
TCACCTGCAT TTGCCACAAG TTTTGGTGTC AAAGCTGTGA TGAAAACCTC TGTCCTTCTC 
ACCTGGGAGG TGCCAGAAAA TTATAAATCA CAAGTGCCTT TTAAGATCCT CTATAACCAG 
CAGAGTGTGG AAGTCCAGGG GAATCTGAAG AGGAAGCTGA TCACACGCCT GCAGCCGGAC 
ACTGATTACT CGTTTGTGCT GATGAGCCGG GGAAACAGCG CCGGTGGCCT ACAACAGCAG 
GTCTCCATCC GCACTGCACC AGACCTGTTC AAAACTAAAC CTGTCCTGTA CACGGCAGAC 
CAGACCAACA ATGGCAAACT CACCATCAAC CTGCCCAAAG TGCCCACCTC TGCTCCTGTC 
AGGTGTTATT ACATTGTGGT CGTGCCCGTC TCTCAGACAT CATCTCGTCA GTGGATTAAT 
CCCGATGAGA TGGAGCTGGA TGAGCTCCTG GAGTCTAGTG AGGGAGCTCA TCTCAGGAGA 
CGTCGACGGC ACACAGACAC CGTAAGACCG TACATCGCCG CAAAGCTATC CACGCTCCCA 
GAAACCTTCA CTCTGGGCGA TGAGCTAGAC TACAGCGGCT TCATCAACCG GCCTCTTCCC 
AACAACCAAC ACTTCCAGTG TTTTGTGATG GCTGAACTCA AGGACCAATA CCCTGTTACT 
GCCAATGAGA AGCAGCGGAC CTTCTCGGCT AGCCCATACT CAGACCCCAT CATAGTGCAG 
GGTGAAAACC AAATGCAGCG CAGTGTGGAC CAACCGGAGA TGCTTTGGGT CATGGGCCCT 
GTGCTCGCTG TGGTGCTCAT CATCATCATA GTCATCGCCA TCCTGCTCTT CAAAAGCAAA 
CAGGAGAGGA AGAGGGCTTC CCCCCTACCA AAAGACGATC ATTCGGGTGG TGTGAAGGAT 
TGTCTTCTGG CCAGCTCATC CGATCCTGTC GAAATGAGGA GACTCAACTA CCAGACACCA 
GGAATGAGGG AACATCCTCC CATATCCGTC TCCGAACTGG CAGACCACAT CGAGCGCCTC 
AAGGCCAACG ATGCCCTCCG GTTTTCCCAA GAGTATGAGT CTATTGATCC AGGCCAGCAG 
TTCACATGGG AGAACTCCAA CTTGGAGGTC AACAAGCCAA AGAACCGTTA TGCCAACGTT 
ATTGCTTACG ACCACTCGAG AGTTGTTCTC ACCCCTGTGG ACGGTGTACC AGGGAGTGAC 
TACATTAATT CCAACTACAT TGATGGCTAC CGTAAGCAGA ATGCCTACAT TGCCACACAA 
GGTCCTCTGC CTGAGACTCT GAGCGACTTC TGGAGGATGA TTTGGGAGCA ACGAGCCAGT 
ACCATCGTCA TGATGACCAG ACTGGAAGAG AAGTCCAGAG TCAAGTGTGA CCAATACTGG 
CCCATTCGTG GCACAGAGAC TTATGGAATG ATTCAAGTGA CCATGCTTGA TGCTGTTGAG 
CTGGCAACCT ACAGTGTGCG CACCTTTGCC CTTTACAAGA ATGGCTCTAG TGAGAAGCGC 
GAGGTCAGGC AGTTTCAGTT CATGGCTTGG CCGGACCACG GAGTGCCTGA ATATCCCACC 
CCCATCCTAG CCTTCCTGCG CAGGGTCAAA GCCTGCAACC CTCCTGATGC TGGACCCATG 
GTAGTGCACT GCAGTGCTGG AGTTGGCCGT ACAGGCTGCT TCATAGTCAT CGATGCGATG 
TTGGAGCGCA TGAAGCACGA GAAGACCGTA GACATTTATG GCCACGTCAC GTGCATGAGA 
TCCCAGAGGA ACTATATGGT GCAGACGGAG GACCAGTACA TCTTTATCCA CGAGGCCCTT 
CTGGAGGCCG CCACCTGTGG AAACACAGAG GTCCCTGCCC GAAACCTCTA CGCCCACATC 
CAGAAGCTCA CACAGCCATC GGCTGGAGAG ACCGTCACAG CCATGGAGCT GGAGTTTAAG 
AGACTCGCCA ACTCCAAAGC CCACACCTCA AGATTCATCA GCGCCAACCT GCCCTGCAAC 
AAGTTCAAGA ACCGGCTGGT CAATATCATG CCTTTCGAGT CCACACGGGT GTGCCTGCAG 
CCAATCCGAG GAGTGGAGGG GTCCGACTAC ATCAATGCCA GCTGCATTGA CGGATACAGA 
CAGCAGAAGG CCTATATTGC CACTCAGGGT CCTCTAGCGG AGACCACCGA GGACTTCTGG 
AGGATGTTAT GGGAGCACAA TTCCACTATT GTAGTGATGC TGACCAAACT CAGAGAGATG 
GGACGGGAGA AGTGTCATCA GTACTGGCCC GCCGAGCGCT CAGCCCGCTA TCAGTACTTC 
GTAGTGGATC CCATGGCCGA ATACAACATG CCCCAGTACA TCCTGCGAGA GTTCAAAGTC 
ACAGATGCCA GGGACGGTCA ATCCAGAACC ATTCGCCAGT TCCAGTTCAC TGATTGGCCA 
GAGCAGGGAG TGCCAAAAAC CGGTGAGGGG TTCATCGATT TCATTGGGCA AGTTCATAAA 
ACCAAGGAGC AGTTTGGGCA GGATGGACCA ATCACTGTGC ACTGCAGTGC TGGAGTGGGA 
AGAACAGGTG TTTTCATCAC TCTCAGTATT GTCCTGGAGA GGATGAGATA TGAGGGGGTG 
GTGGATCTGT TCCAGACGGT TAAAACCCTG CGCACACAAA GGCCTGCCAT GGTGCAGACA 
GAGGACCAGT ACCAGCTGTG CTACAGAGCC GCTCTGGAGT ACCTGGGGAG CTTTGATCAC 
TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296735.1
CDS	426..6197
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRDPRGVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298460.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298460.2

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG ACCCTCGAGG AGTACGACGA 
GTTCCACCCC GTTTCTCCAT CCCACCTACC AATCACGAGG TGATGCCAGG GGGCAGTGTA 
AACCTCACCT GCGTGGCAGT AGGTGCCCCG ATGCCCTACG TCAAATGGAT GACTGGTGAG 
GTGGAGCTCA CCAAGGATGA GGAGATGCCT GTGGGACGCA ACGTCTTGGA GCTCACCAAC 
ATCCGTCAGT CGACCAACTA CACCTGCGTG GCTATATCAT CCCTAGGTAT GATCGAGGCA 
ACGGCACAAG TGTCTGTCAA AGCTCTCCCA AAGCCTCCTA CATCCCTGAC TGTTACGGAA 
ACCACAGCTA CGAGTGTTAC ACTCACATGG GACTCTGGAA ACCCTGAACC TGTGTCTTAC 
TACATAATTC AATACCGGGC CAAGACTTCA GACACCAACT TCCAAGAAGT GGACGGTGTG 
GCCACCACAC GTTACAGCAT CGGCGGTCTC AGCCCATATT CAGAGTATGA GTTTCGAGTA 
ATGGCAGTCA ACAACATTGG TCGTGGGCCA CCTAGTGAAC CCGTTGAAAC ACGAACAGGT 
GAACAGGCAC CTTCCTCCCC ACCGCTGCAT GTTCAAGCAC GAATGCTGAG TGCCAGCACC 
ATGTTGGTTC AGTGGGAACC ACCAGAGGAA CCCAATGGTC AGATCAGAGG CTATCGGATC 
TACTACACAT CTGACCTGGA TGCACCTCTT AGCGCCTGGC AGAAACACAA CACAGACGAT 
AGCCGTCTCA CCACTATCTC CAGCCTGACC ACCGGCATCA CCTACAGCCT ACGTGTGCTG 
GGCTTCACAT CTGTAGGGGA TGGACCACCA TCTGATGTGC TACAGGTCAA GACACAGCAA 
GGAGTTCCTG CTCAGCCATC CAGCTTCGAA GCTGAAGCAG AGCTAGATAC AAGGATCCTA 
CTAACTTGGC TGTGGCCCGT GCAGGACCCC ATCATCAAAT ACGAGCTGCA ATATTGGGAG 
GCTGACTCAG ACAACAAGAT CCATGTCACC TTTGACCCAG CTGGCTCGTA CGCTGTTGAA 
GGTCTGAAAC CAGACACTTT GTACAAATTC TCTCTGGCAG CCCGCTCTGA AATGGGACAA 
GGAGTCTTCA CTCACCCCAT TGAAGCCAGG ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT 
CAAGACGTGC ACCTGCTCAG TCTGAGCTCC ACCAGCATCA AGGTGAGTTG GGTGGCACCA 
CCCGCCGCTA GCCGCCACGG CGCAATAGTG CGCTACACAG TCAGCTACCA GGCAGTGAAT 
GGAGAAGACA CAGAGCGCCA TGAGGTGCCG GACATTGGAG CGGATGCCTC CAGCTGTGTG 
TTGGAAGGTC TAGAGAAGTG GACGGAGTAT CAGGTGTGGG TGAGAGCGCA CACTGATGTG 
GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT ATCCGAACCA ATGAAGACGT GCCAGGAGCA 
CCGCCTCGGA AATTGGAAGT GGAGGCTTTG AACTCCACCG CTATCCGTGT GACATGGAAA 
CCTCCTCTTT CTGGAAAGCA GCATGGCCAG ATCCGGGGAT ATCAGGTCAT ATACTCCCGG 
TTGGAGAACG GGGAACCACG GGGACATCCG AATATAATGG ATGTCGCTCT GCCAGAAGCA 
CAGTGGAAAA TTGAAGAGTC TACGGAATAT GAGGCTGTGA TCGCTGGACT TGCCTCTGAA 
ACCTCTTACT CAGTGACTGT GGCTGCGTAC ACAACCAAAG GTGATGGAGC TCGCAGCAAA 
GCCAAAGTGG TCACCACCAC CGGAGCAGTG CCTGGCAAAC CCACTATGAT CATCAGCACC 
ACCGTTGGCA ACACGGCCCT TATCCAGTGG CAGCCTCCTA AAGACATGGT GGGTGAGCTG 
ATGGGCTACC GGCTGCGCTA CAAACGTCTG GAAGAGGAAA ACTATGAGAT ACGCGAATTC 
AGTCGCACCG ACGACCACCA CACCGTCACT GACCTGCACA AGGGCGCCAC CTACAGCTTC 
CACCTGAGCG CCAGAAACCG CGCTGGACTC GGAGAGGAGT ACGTTAAAGA CATCGGCACT 
CCTGAGGACG TGCCCAGCGG GTTTCCTCTT AACCTGCAGG TCGTAGGCTT GACTACCTCT 
ACCACCCGAC TGACCTGGGA GCCTCCAGCG TTGTCTGAAC GTAACGGACG GATCATACAT 
TACATTGTGG TTTACCGAGA CATCAACAGC AAACAAAACA GCACCAACCG AACCTCCGAC 
ACACAGATGA CCATCCAAGG GCTCCGACCT GACACCACCT ATGATATCCG TGTGCAGGCG 
TTCACTAGCA AGGGTGGAGG TCCCATAAGT CCCAGTATCC AGAGCAGGAC CATGTCTACG 
TCACCTGCAT TTGCCACAAG TTTTGGTGTC AAAGCTGTGA TGAAAACCTC TGTCCTTCTC 
ACCTGGGAGG TGCCAGAAAA TTATAAATCA CAAGTGCCTT TTAAGATCCT CTATAACCAG 
CAGAGTGTGG AAGTCCAGGG GAATCTGAAG AGGAAGCTGA TCACACGCCT GCAGCCGGAC 
ACTGATTACT CGTTTGTGCT GATGAGCCGG GGAAACAGCG CCGGTGGCCT ACAACAGCAG 
GTCTCCATCC GCACTGCACC AGACCTGTTC AAAACTAAAC CTGTCCTGTA CACGGCAGAC 
CAGACCAACA ATGGCAAACT CACCATCAAC CTGCCCAAAG TGCCCACCTC TGCTCCTGTC 
AGGTGTTATT ACATTGTGGT CGTGCCCGTC TCTCAGACAT CATCTCGTCA GTGGATTAAT 
CCCGATGAGA TGGAGCTGGA TGAGCTCCTG GAGTCTAGTG AGGGAGCTCA TCTCAGGAGA 
CGTCGACGGC ACACAGACAC CGTAAGACCG TACATCGCCG CAAAGCTATC CACGCTCCCA 
GAAACCTTCA CTCTGGGCGA TGAGCTAGAC TACAGCGGCT TCATCAACCG GCCTCTTCCC 
AACAACCAAC ACTTCCAGTG TTTTGTGATG GCTGAACTCA AGGACCAATA CCCTGTTACT 
GCCAATGAGA AGCAGCGGAC CTTCTCGGCT AGCCCATACT CAGACCCCAT CATAGTGCAG 
GGTGAAAACC AAATGCAGCG CAGTGTGGAC CAACCGGAGA TGCTTTGGGT CATGGGCCCT 
GTGCTCGCTG TGGTGCTCAT CATCATCATA GTCATCGCCA TCCTGCTCTT CAAAAGCAAA 
CAGGAGAGGA AGAGGGCTTC CCCCCTACCA AAAGACGATC ATTCGGGTGG TGTGAAGGAT 
TGTCTTCTGG CCAGCTCATC CGATCCTGTC GAAATGAGGA GACTCAACTA CCAGACACCA 
GGAATGAGGG AACATCCTCC CATATCCGTC TCCGAACTGG CAGACCACAT CGAGCGCCTC 
AAGGCCAACG ATGCCCTCCG GTTTTCCCAA GAGTATGAGT CTATTGATCC AGGCCAGCAG 
TTCACATGGG AGAACTCCAA CTTGGAGGTC AACAAGCCAA AGAACCGTTA TGCCAACGTT 
ATTGCTTACG ACCACTCGAG AGTTGTTCTC ACCCCTGTGG ACGGTGTACC AGGGAGTGAC 
TACATTAATT CCAACTACAT TGATGGCTAC CGTAAGCAGA ATGCCTACAT TGCCACACAA 
GGTCCTCTGC CTGAGACTCT GAGCGACTTC TGGAGGATGA TTTGGGAGCA ACGAGCCAGT 
ACCATCGTCA TGATGACCAG ACTGGAAGAG AAGTCCAGAG TCAAGTGTGA CCAATACTGG 
CCCATTCGTG GCACAGAGAC TTATGGAATG ATTCAAGTGA CCATGCTTGA TGCTGTTGAG 
CTGGCAACCT ACAGTGTGCG CACCTTTGCC CTTTACAAGA ATGGCTCTAG TGAGAAGCGC 
GAGGTCAGGC AGTTTCAGTT CATGGCTTGG CCGGACCACG GAGTGCCTGA ATATCCCACC 
CCCATCCTAG CCTTCCTGCG CAGGGTCAAA GCCTGCAACC CTCCTGATGC TGGACCCATG 
GTAGTGCACT GCAGTGCTGG AGTTGGCCGT ACAGGCTGCT TCATAGTCAT CGATGCGATG 
TTGGAGCGCA TGAAGCACGA GAAGACCGTA GACATTTATG GCCACGTCAC GTGCATGAGA 
TCCCAGAGGA ACTATATGGT GCAGACGGAG GACCAGTACA TCTTTATCCA CGAGGCCCTT 
CTGGAGGCCG CCACCTGTGG AAACACAGAG GTCCCTGCCC GAAACCTCTA CGCCCACATC 
CAGAAGCTCA CACAGCCATC GGCTGGAGAG ACCGTCACAG CCATGGAGCT GGAGTTTAAG 
AGACTCGCCA ACTCCAAAGC CCACACCTCA AGATTCATCA GCGCCAACCT GCCCTGCAAC 
AAGTTCAAGA ACCGGCTGGT CAATATCATG CCTTTCGAGT CCACACGGGT GTGCCTGCAG 
CCAATCCGAG GAGTGGAGGG GTCCGACTAC ATCAATGCCA GCTGCATTGA CGGATACAGA 
CAGCAGAAGG CCTATATTGC CACTCAGGGT CCTCTAGCGG AGACCACCGA GGACTTCTGG 
AGGATGTTAT GGGAGCACAA TTCCACTATT GTAGTGATGC TGACCAAACT CAGAGAGATG 
GGACGGGAGA AGTGTCATCA GTACTGGCCC GCCGAGCGCT CAGCCCGCTA TCAGTACTTC 
GTAGTGGATC CCATGGCCGA ATACAACATG CCCCAGTACA TCCTGCGAGA GTTCAAAGTC 
ACAGATGCCA GGGACGGTCA ATCCAGAACC ATTCGCCAGT TCCAGTTCAC TGATTGGCCA 
GAGCAGGGAG TGCCAAAAAC CGGTGAGGGG TTCATCGATT TCATTGGGCA AGTTCATAAA 
ACCAAGGAGC AGTTTGGGCA GGATGGACCA ATCACTGTGC ACTGCAGTGC TGGAGTGGGA 
AGAACAGGTG TTTTCATCAC TCTCAGTATT GTCCTGGAGA GGATGAGATA TGAGGGGGTG 
GTGGATCTGT TCCAGACGGT TAAAACCCTG CGCACACAAA GGCCTGCCAT GGTGCAGACA 
GAGGACCAGT ACCAGCTGTG CTACAGAGCC GCTCTGGAGT ACCTGGGGAG CTTTGATCAC 
TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298460.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296735.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X5, mRNA.

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CloneID	ODa47415
Clone ID Related Accession (Same CDS sequence)	XM_009298460.2 , XM_009298460.3
Accession Version	XM_009298460.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298460.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG ACCCTCGAGG AGTACGACGA 
GTTCCACCCC GTTTCTCCAT CCCACCTACC AATCACGAGG TGATGCCAGG GGGCAGTGTA 
AACCTCACCT GCGTGGCAGT AGGTGCCCCG ATGCCCTACG TCAAATGGAT GACTGGTGAG 
GTGGAGCTCA CCAAGGATGA GGAGATGCCT GTGGGACGCA ACGTCTTGGA GCTCACCAAC 
ATCCGTCAGT CGACCAACTA CACCTGCGTG GCTATATCAT CCCTAGGTAT GATCGAGGCA 
ACGGCACAAG TGTCTGTCAA AGCTCTCCCA AAGCCTCCTA CATCCCTGAC TGTTACGGAA 
ACCACAGCTA CGAGTGTTAC ACTCACATGG GACTCTGGAA ACCCTGAACC TGTGTCTTAC 
TACATAATTC AATACCGGGC CAAGACTTCA GACACCAACT TCCAAGAAGT GGACGGTGTG 
GCCACCACAC GTTACAGCAT CGGCGGTCTC AGCCCATATT CAGAGTATGA GTTTCGAGTA 
ATGGCAGTCA ACAACATTGG TCGTGGGCCA CCTAGTGAAC CCGTTGAAAC ACGAACAGGT 
GAACAGGCAC CTTCCTCCCC ACCGCTGCAT GTTCAAGCAC GAATGCTGAG TGCCAGCACC 
ATGTTGGTTC AGTGGGAACC ACCAGAGGAA CCCAATGGTC AGATCAGAGG CTATCGGATC 
TACTACACAT CTGACCTGGA TGCACCTCTT AGCGCCTGGC AGAAACACAA CACAGACGAT 
AGCCGTCTCA CCACTATCTC CAGCCTGACC ACCGGCATCA CCTACAGCCT ACGTGTGCTG 
GGCTTCACAT CTGTAGGGGA TGGACCACCA TCTGATGTGC TACAGGTCAA GACACAGCAA 
GGAGTTCCTG CTCAGCCATC CAGCTTCGAA GCTGAAGCAG AGCTAGATAC AAGGATCCTA 
CTAACTTGGC TGTGGCCCGT GCAGGACCCC ATCATCAAAT ACGAGCTGCA ATATTGGGAG 
GCTGACTCAG ACAACAAGAT CCATGTCACC TTTGACCCAG CTGGCTCGTA CGCTGTTGAA 
GGTCTGAAAC CAGACACTTT GTACAAATTC TCTCTGGCAG CCCGCTCTGA AATGGGACAA 
GGAGTCTTCA CTCACCCCAT TGAAGCCAGG ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT 
CAAGACGTGC ACCTGCTCAG TCTGAGCTCC ACCAGCATCA AGGTGAGTTG GGTGGCACCA 
CCCGCCGCTA GCCGCCACGG CGCAATAGTG CGCTACACAG TCAGCTACCA GGCAGTGAAT 
GGAGAAGACA CAGAGCGCCA TGAGGTGCCG GACATTGGAG CGGATGCCTC CAGCTGTGTG 
TTGGAAGGTC TAGAGAAGTG GACGGAGTAT CAGGTGTGGG TGAGAGCGCA CACTGATGTG 
GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT ATCCGAACCA ATGAAGACGT GCCAGGAGCA 
CCGCCTCGGA AATTGGAAGT GGAGGCTTTG AACTCCACCG CTATCCGTGT GACATGGAAA 
CCTCCTCTTT CTGGAAAGCA GCATGGCCAG ATCCGGGGAT ATCAGGTCAT ATACTCCCGG 
TTGGAGAACG GGGAACCACG GGGACATCCG AATATAATGG ATGTCGCTCT GCCAGAAGCA 
CAGTGGAAAA TTGAAGAGTC TACGGAATAT GAGGCTGTGA TCGCTGGACT TGCCTCTGAA 
ACCTCTTACT CAGTGACTGT GGCTGCGTAC ACAACCAAAG GTGATGGAGC TCGCAGCAAA 
GCCAAAGTGG TCACCACCAC CGGAGCAGTG CCTGGCAAAC CCACTATGAT CATCAGCACC 
ACCGTTGGCA ACACGGCCCT TATCCAGTGG CAGCCTCCTA AAGACATGGT GGGTGAGCTG 
ATGGGCTACC GGCTGCGCTA CAAACGTCTG GAAGAGGAAA ACTATGAGAT ACGCGAATTC 
AGTCGCACCG ACGACCACCA CACCGTCACT GACCTGCACA AGGGCGCCAC CTACAGCTTC 
CACCTGAGCG CCAGAAACCG CGCTGGACTC GGAGAGGAGT ACGTTAAAGA CATCGGCACT 
CCTGAGGACG TGCCCAGCGG GTTTCCTCTT AACCTGCAGG TCGTAGGCTT GACTACCTCT 
ACCACCCGAC TGACCTGGGA GCCTCCAGCG TTGTCTGAAC GTAACGGACG GATCATACAT 
TACATTGTGG TTTACCGAGA CATCAACAGC AAACAAAACA GCACCAACCG AACCTCCGAC 
ACACAGATGA CCATCCAAGG GCTCCGACCT GACACCACCT ATGATATCCG TGTGCAGGCG 
TTCACTAGCA AGGGTGGAGG TCCCATAAGT CCCAGTATCC AGAGCAGGAC CATGTCTACG 
TCACCTGCAT TTGCCACAAG TTTTGGTGTC AAAGCTGTGA TGAAAACCTC TGTCCTTCTC 
ACCTGGGAGG TGCCAGAAAA TTATAAATCA CAAGTGCCTT TTAAGATCCT CTATAACCAG 
CAGAGTGTGG AAGTCCAGGG GAATCTGAAG AGGAAGCTGA TCACACGCCT GCAGCCGGAC 
ACTGATTACT CGTTTGTGCT GATGAGCCGG GGAAACAGCG CCGGTGGCCT ACAACAGCAG 
GTCTCCATCC GCACTGCACC AGACCTGTTC AAAACTAAAC CTGTCCTGTA CACGGCAGAC 
CAGACCAACA ATGGCAAACT CACCATCAAC CTGCCCAAAG TGCCCACCTC TGCTCCTGTC 
AGGTGTTATT ACATTGTGGT CGTGCCCGTC TCTCAGACAT CATCTCGTCA GTGGATTAAT 
CCCGATGAGA TGGAGCTGGA TGAGCTCCTG GAGTCTAGTG AGGGAGCTCA TCTCAGGAGA 
CGTCGACGGC ACACAGACAC CGTAAGACCG TACATCGCCG CAAAGCTATC CACGCTCCCA 
GAAACCTTCA CTCTGGGCGA TGAGCTAGAC TACAGCGGCT TCATCAACCG GCCTCTTCCC 
AACAACCAAC ACTTCCAGTG TTTTGTGATG GCTGAACTCA AGGACCAATA CCCTGTTACT 
GCCAATGAGA AGCAGCGGAC CTTCTCGGCT AGCCCATACT CAGACCCCAT CATAGTGCAG 
GGTGAAAACC AAATGCAGCG CAGTGTGGAC CAACCGGAGA TGCTTTGGGT CATGGGCCCT 
GTGCTCGCTG TGGTGCTCAT CATCATCATA GTCATCGCCA TCCTGCTCTT CAAAAGCAAA 
CAGGAGAGGA AGAGGGCTTC CCCCCTACCA AAAGACGATC ATTCGGGTGG TGTGAAGGAT 
TGTCTTCTGG CCAGCTCATC CGATCCTGTC GAAATGAGGA GACTCAACTA CCAGACACCA 
GGAATGAGGG AACATCCTCC CATATCCGTC TCCGAACTGG CAGACCACAT CGAGCGCCTC 
AAGGCCAACG ATGCCCTCCG GTTTTCCCAA GAGTATGAGT CTATTGATCC AGGCCAGCAG 
TTCACATGGG AGAACTCCAA CTTGGAGGTC AACAAGCCAA AGAACCGTTA TGCCAACGTT 
ATTGCTTACG ACCACTCGAG AGTTGTTCTC ACCCCTGTGG ACGGTGTACC AGGGAGTGAC 
TACATTAATT CCAACTACAT TGATGGCTAC CGTAAGCAGA ATGCCTACAT TGCCACACAA 
GGTCCTCTGC CTGAGACTCT GAGCGACTTC TGGAGGATGA TTTGGGAGCA ACGAGCCAGT 
ACCATCGTCA TGATGACCAG ACTGGAAGAG AAGTCCAGAG TCAAGTGTGA CCAATACTGG 
CCCATTCGTG GCACAGAGAC TTATGGAATG ATTCAAGTGA CCATGCTTGA TGCTGTTGAG 
CTGGCAACCT ACAGTGTGCG CACCTTTGCC CTTTACAAGA ATGGCTCTAG TGAGAAGCGC 
GAGGTCAGGC AGTTTCAGTT CATGGCTTGG CCGGACCACG GAGTGCCTGA ATATCCCACC 
CCCATCCTAG CCTTCCTGCG CAGGGTCAAA GCCTGCAACC CTCCTGATGC TGGACCCATG 
GTAGTGCACT GCAGTGCTGG AGTTGGCCGT ACAGGCTGCT TCATAGTCAT CGATGCGATG 
TTGGAGCGCA TGAAGCACGA GAAGACCGTA GACATTTATG GCCACGTCAC GTGCATGAGA 
TCCCAGAGGA ACTATATGGT GCAGACGGAG GACCAGTACA TCTTTATCCA CGAGGCCCTT 
CTGGAGGCCG CCACCTGTGG AAACACAGAG GTCCCTGCCC GAAACCTCTA CGCCCACATC 
CAGAAGCTCA CACAGCCATC GGCTGGAGAG ACCGTCACAG CCATGGAGCT GGAGTTTAAG 
AGACTCGCCA ACTCCAAAGC CCACACCTCA AGATTCATCA GCGCCAACCT GCCCTGCAAC 
AAGTTCAAGA ACCGGCTGGT CAATATCATG CCTTTCGAGT CCACACGGGT GTGCCTGCAG 
CCAATCCGAG GAGTGGAGGG GTCCGACTAC ATCAATGCCA GCTGCATTGA CGGATACAGA 
CAGCAGAAGG CCTATATTGC CACTCAGGGT CCTCTAGCGG AGACCACCGA GGACTTCTGG 
AGGATGTTAT GGGAGCACAA TTCCACTATT GTAGTGATGC TGACCAAACT CAGAGAGATG 
GGACGGGAGA AGTGTCATCA GTACTGGCCC GCCGAGCGCT CAGCCCGCTA TCAGTACTTC 
GTAGTGGATC CCATGGCCGA ATACAACATG CCCCAGTACA TCCTGCGAGA GTTCAAAGTC 
ACAGATGCCA GGGACGGTCA ATCCAGAACC ATTCGCCAGT TCCAGTTCAC TGATTGGCCA 
GAGCAGGGAG TGCCAAAAAC CGGTGAGGGG TTCATCGATT TCATTGGGCA AGTTCATAAA 
ACCAAGGAGC AGTTTGGGCA GGATGGACCA ATCACTGTGC ACTGCAGTGC TGGAGTGGGA 
AGAACAGGTG TTTTCATCAC TCTCAGTATT GTCCTGGAGA GGATGAGATA TGAGGGGGTG 
GTGGATCTGT TCCAGACGGT TAAAACCCTG CGCACACAAA GGCCTGCCAT GGTGCAGACA 
GAGGACCAGT ACCAGCTGTG CTACAGAGCC GCTCTGGAGT ACCTGGGGAG CTTTGATCAC 
TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296735.1
CDS	422..6193
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRDPRGVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298460.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298460.3

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG ACCCTCGAGG AGTACGACGA 
GTTCCACCCC GTTTCTCCAT CCCACCTACC AATCACGAGG TGATGCCAGG GGGCAGTGTA 
AACCTCACCT GCGTGGCAGT AGGTGCCCCG ATGCCCTACG TCAAATGGAT GACTGGTGAG 
GTGGAGCTCA CCAAGGATGA GGAGATGCCT GTGGGACGCA ACGTCTTGGA GCTCACCAAC 
ATCCGTCAGT CGACCAACTA CACCTGCGTG GCTATATCAT CCCTAGGTAT GATCGAGGCA 
ACGGCACAAG TGTCTGTCAA AGCTCTCCCA AAGCCTCCTA CATCCCTGAC TGTTACGGAA 
ACCACAGCTA CGAGTGTTAC ACTCACATGG GACTCTGGAA ACCCTGAACC TGTGTCTTAC 
TACATAATTC AATACCGGGC CAAGACTTCA GACACCAACT TCCAAGAAGT GGACGGTGTG 
GCCACCACAC GTTACAGCAT CGGCGGTCTC AGCCCATATT CAGAGTATGA GTTTCGAGTA 
ATGGCAGTCA ACAACATTGG TCGTGGGCCA CCTAGTGAAC CCGTTGAAAC ACGAACAGGT 
GAACAGGCAC CTTCCTCCCC ACCGCTGCAT GTTCAAGCAC GAATGCTGAG TGCCAGCACC 
ATGTTGGTTC AGTGGGAACC ACCAGAGGAA CCCAATGGTC AGATCAGAGG CTATCGGATC 
TACTACACAT CTGACCTGGA TGCACCTCTT AGCGCCTGGC AGAAACACAA CACAGACGAT 
AGCCGTCTCA CCACTATCTC CAGCCTGACC ACCGGCATCA CCTACAGCCT ACGTGTGCTG 
GGCTTCACAT CTGTAGGGGA TGGACCACCA TCTGATGTGC TACAGGTCAA GACACAGCAA 
GGAGTTCCTG CTCAGCCATC CAGCTTCGAA GCTGAAGCAG AGCTAGATAC AAGGATCCTA 
CTAACTTGGC TGTGGCCCGT GCAGGACCCC ATCATCAAAT ACGAGCTGCA ATATTGGGAG 
GCTGACTCAG ACAACAAGAT CCATGTCACC TTTGACCCAG CTGGCTCGTA CGCTGTTGAA 
GGTCTGAAAC CAGACACTTT GTACAAATTC TCTCTGGCAG CCCGCTCTGA AATGGGACAA 
GGAGTCTTCA CTCACCCCAT TGAAGCCAGG ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT 
CAAGACGTGC ACCTGCTCAG TCTGAGCTCC ACCAGCATCA AGGTGAGTTG GGTGGCACCA 
CCCGCCGCTA GCCGCCACGG CGCAATAGTG CGCTACACAG TCAGCTACCA GGCAGTGAAT 
GGAGAAGACA CAGAGCGCCA TGAGGTGCCG GACATTGGAG CGGATGCCTC CAGCTGTGTG 
TTGGAAGGTC TAGAGAAGTG GACGGAGTAT CAGGTGTGGG TGAGAGCGCA CACTGATGTG 
GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT ATCCGAACCA ATGAAGACGT GCCAGGAGCA 
CCGCCTCGGA AATTGGAAGT GGAGGCTTTG AACTCCACCG CTATCCGTGT GACATGGAAA 
CCTCCTCTTT CTGGAAAGCA GCATGGCCAG ATCCGGGGAT ATCAGGTCAT ATACTCCCGG 
TTGGAGAACG GGGAACCACG GGGACATCCG AATATAATGG ATGTCGCTCT GCCAGAAGCA 
CAGTGGAAAA TTGAAGAGTC TACGGAATAT GAGGCTGTGA TCGCTGGACT TGCCTCTGAA 
ACCTCTTACT CAGTGACTGT GGCTGCGTAC ACAACCAAAG GTGATGGAGC TCGCAGCAAA 
GCCAAAGTGG TCACCACCAC CGGAGCAGTG CCTGGCAAAC CCACTATGAT CATCAGCACC 
ACCGTTGGCA ACACGGCCCT TATCCAGTGG CAGCCTCCTA AAGACATGGT GGGTGAGCTG 
ATGGGCTACC GGCTGCGCTA CAAACGTCTG GAAGAGGAAA ACTATGAGAT ACGCGAATTC 
AGTCGCACCG ACGACCACCA CACCGTCACT GACCTGCACA AGGGCGCCAC CTACAGCTTC 
CACCTGAGCG CCAGAAACCG CGCTGGACTC GGAGAGGAGT ACGTTAAAGA CATCGGCACT 
CCTGAGGACG TGCCCAGCGG GTTTCCTCTT AACCTGCAGG TCGTAGGCTT GACTACCTCT 
ACCACCCGAC TGACCTGGGA GCCTCCAGCG TTGTCTGAAC GTAACGGACG GATCATACAT 
TACATTGTGG TTTACCGAGA CATCAACAGC AAACAAAACA GCACCAACCG AACCTCCGAC 
ACACAGATGA CCATCCAAGG GCTCCGACCT GACACCACCT ATGATATCCG TGTGCAGGCG 
TTCACTAGCA AGGGTGGAGG TCCCATAAGT CCCAGTATCC AGAGCAGGAC CATGTCTACG 
TCACCTGCAT TTGCCACAAG TTTTGGTGTC AAAGCTGTGA TGAAAACCTC TGTCCTTCTC 
ACCTGGGAGG TGCCAGAAAA TTATAAATCA CAAGTGCCTT TTAAGATCCT CTATAACCAG 
CAGAGTGTGG AAGTCCAGGG GAATCTGAAG AGGAAGCTGA TCACACGCCT GCAGCCGGAC 
ACTGATTACT CGTTTGTGCT GATGAGCCGG GGAAACAGCG CCGGTGGCCT ACAACAGCAG 
GTCTCCATCC GCACTGCACC AGACCTGTTC AAAACTAAAC CTGTCCTGTA CACGGCAGAC 
CAGACCAACA ATGGCAAACT CACCATCAAC CTGCCCAAAG TGCCCACCTC TGCTCCTGTC 
AGGTGTTATT ACATTGTGGT CGTGCCCGTC TCTCAGACAT CATCTCGTCA GTGGATTAAT 
CCCGATGAGA TGGAGCTGGA TGAGCTCCTG GAGTCTAGTG AGGGAGCTCA TCTCAGGAGA 
CGTCGACGGC ACACAGACAC CGTAAGACCG TACATCGCCG CAAAGCTATC CACGCTCCCA 
GAAACCTTCA CTCTGGGCGA TGAGCTAGAC TACAGCGGCT TCATCAACCG GCCTCTTCCC 
AACAACCAAC ACTTCCAGTG TTTTGTGATG GCTGAACTCA AGGACCAATA CCCTGTTACT 
GCCAATGAGA AGCAGCGGAC CTTCTCGGCT AGCCCATACT CAGACCCCAT CATAGTGCAG 
GGTGAAAACC AAATGCAGCG CAGTGTGGAC CAACCGGAGA TGCTTTGGGT CATGGGCCCT 
GTGCTCGCTG TGGTGCTCAT CATCATCATA GTCATCGCCA TCCTGCTCTT CAAAAGCAAA 
CAGGAGAGGA AGAGGGCTTC CCCCCTACCA AAAGACGATC ATTCGGGTGG TGTGAAGGAT 
TGTCTTCTGG CCAGCTCATC CGATCCTGTC GAAATGAGGA GACTCAACTA CCAGACACCA 
GGAATGAGGG AACATCCTCC CATATCCGTC TCCGAACTGG CAGACCACAT CGAGCGCCTC 
AAGGCCAACG ATGCCCTCCG GTTTTCCCAA GAGTATGAGT CTATTGATCC AGGCCAGCAG 
TTCACATGGG AGAACTCCAA CTTGGAGGTC AACAAGCCAA AGAACCGTTA TGCCAACGTT 
ATTGCTTACG ACCACTCGAG AGTTGTTCTC ACCCCTGTGG ACGGTGTACC AGGGAGTGAC 
TACATTAATT CCAACTACAT TGATGGCTAC CGTAAGCAGA ATGCCTACAT TGCCACACAA 
GGTCCTCTGC CTGAGACTCT GAGCGACTTC TGGAGGATGA TTTGGGAGCA ACGAGCCAGT 
ACCATCGTCA TGATGACCAG ACTGGAAGAG AAGTCCAGAG TCAAGTGTGA CCAATACTGG 
CCCATTCGTG GCACAGAGAC TTATGGAATG ATTCAAGTGA CCATGCTTGA TGCTGTTGAG 
CTGGCAACCT ACAGTGTGCG CACCTTTGCC CTTTACAAGA ATGGCTCTAG TGAGAAGCGC 
GAGGTCAGGC AGTTTCAGTT CATGGCTTGG CCGGACCACG GAGTGCCTGA ATATCCCACC 
CCCATCCTAG CCTTCCTGCG CAGGGTCAAA GCCTGCAACC CTCCTGATGC TGGACCCATG 
GTAGTGCACT GCAGTGCTGG AGTTGGCCGT ACAGGCTGCT TCATAGTCAT CGATGCGATG 
TTGGAGCGCA TGAAGCACGA GAAGACCGTA GACATTTATG GCCACGTCAC GTGCATGAGA 
TCCCAGAGGA ACTATATGGT GCAGACGGAG GACCAGTACA TCTTTATCCA CGAGGCCCTT 
CTGGAGGCCG CCACCTGTGG AAACACAGAG GTCCCTGCCC GAAACCTCTA CGCCCACATC 
CAGAAGCTCA CACAGCCATC GGCTGGAGAG ACCGTCACAG CCATGGAGCT GGAGTTTAAG 
AGACTCGCCA ACTCCAAAGC CCACACCTCA AGATTCATCA GCGCCAACCT GCCCTGCAAC 
AAGTTCAAGA ACCGGCTGGT CAATATCATG CCTTTCGAGT CCACACGGGT GTGCCTGCAG 
CCAATCCGAG GAGTGGAGGG GTCCGACTAC ATCAATGCCA GCTGCATTGA CGGATACAGA 
CAGCAGAAGG CCTATATTGC CACTCAGGGT CCTCTAGCGG AGACCACCGA GGACTTCTGG 
AGGATGTTAT GGGAGCACAA TTCCACTATT GTAGTGATGC TGACCAAACT CAGAGAGATG 
GGACGGGAGA AGTGTCATCA GTACTGGCCC GCCGAGCGCT CAGCCCGCTA TCAGTACTTC 
GTAGTGGATC CCATGGCCGA ATACAACATG CCCCAGTACA TCCTGCGAGA GTTCAAAGTC 
ACAGATGCCA GGGACGGTCA ATCCAGAACC ATTCGCCAGT TCCAGTTCAC TGATTGGCCA 
GAGCAGGGAG TGCCAAAAAC CGGTGAGGGG TTCATCGATT TCATTGGGCA AGTTCATAAA 
ACCAAGGAGC AGTTTGGGCA GGATGGACCA ATCACTGTGC ACTGCAGTGC TGGAGTGGGA 
AGAACAGGTG TTTTCATCAC TCTCAGTATT GTCCTGGAGA GGATGAGATA TGAGGGGGTG 
GTGGATCTGT TCCAGACGGT TAAAACCCTG CGCACACAAA GGCCTGCCAT GGTGCAGACA 
GAGGACCAGT ACCAGCTGTG CTACAGAGCC GCTCTGGAGT ACCTGGGGAG CTTTGATCAC 
TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298461.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296736.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X6, mRNA.

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CloneID	ODa47416
Clone ID Related Accession (Same CDS sequence)	XM_009298461.3 , XM_009298461.2
Accession Version	XM_009298461.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298461.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTCATTCGA GAAGACCCTC GAGGAGTACG ACGAGTTCCA 
CCCCGTTTCT CCATCCCACC TACCAATCAC GAGGTGATGC CAGGGGGCAG TGTAAACCTC 
ACCTGCGTGG CAGTAGGTGC CCCGATGCCC TACGTCAAAT GGATGACTGG TGAGGTGGAG 
CTCACCAAGG ATGAGGAGAT GCCTGTGGGA CGCAACGTCT TGGAGCTCAC CAACATCCGT 
CAGTCGACCA ACTACACCTG CGTGGCTATA TCATCCCTAG GTATGATCGA GGCAACGGCA 
CAAGTGTCTG TCAAAGCTCT CCCAAAGCCT CCTACATCCC TGACTGTTAC GGAAACCACA 
GCTACGAGTG TTACACTCAC ATGGGACTCT GGAAACCCTG AACCTGTGTC TTACTACATA 
ATTCAATACC GGGCCAAGAC TTCAGACACC AACTTCCAAG AAGTGGACGG TGTGGCCACC 
ACACGTTACA GCATCGGCGG TCTCAGCCCA TATTCAGAGT ATGAGTTTCG AGTAATGGCA 
GTCAACAACA TTGGTCGTGG GCCACCTAGT GAACCCGTTG AAACACGAAC AGGTGAACAG 
GCACCTTCCT CCCCACCGCT GCATGTTCAA GCACGAATGC TGAGTGCCAG CACCATGTTG 
GTTCAGTGGG AACCACCAGA GGAACCCAAT GGTCAGATCA GAGGCTATCG GATCTACTAC 
ACATCTGACC TGGATGCACC TCTTAGCGCC TGGCAGAAAC ACAACACAGA CGATAGCCGT 
CTCACCACTA TCTCCAGCCT GACCACCGGC ATCACCTACA GCCTACGTGT GCTGGGCTTC 
ACATCTGTAG GGGATGGACC ACCATCTGAT GTGCTACAGG TCAAGACACA GCAAGGAGTT 
CCTGCTCAGC CATCCAGCTT CGAAGCTGAA GCAGAGCTAG ATACAAGGAT CCTACTAACT 
TGGCTGTGGC CCGTGCAGGA CCCCATCATC AAATACGAGC TGCAATATTG GGAGGCTGAC 
TCAGACAACA AGATCCATGT CACCTTTGAC CCAGCTGGCT CGTACGCTGT TGAAGGTCTG 
AAACCAGACA CTTTGTACAA ATTCTCTCTG GCAGCCCGCT CTGAAATGGG ACAAGGAGTC 
TTCACTCACC CCATTGAAGC CAGGACGGCC CAGTCTGCCC CCTCTGCCCC CCCTCAAGAC 
GTGCACCTGC TCAGTCTGAG CTCCACCAGC ATCAAGGTGA GTTGGGTGGC ACCACCCGCC 
GCTAGCCGCC ACGGCGCAAT AGTGCGCTAC ACAGTCAGCT ACCAGGCAGT GAATGGAGAA 
GACACAGAGC GCCATGAGGT GCCGGACATT GGAGCGGATG CCTCCAGCTG TGTGTTGGAA 
GGTCTAGAGA AGTGGACGGA GTATCAGGTG TGGGTGAGAG CGCACACTGA TGTGGGCCCC 
GGCCCCGAGA GCACGGCTGT GCGTATCCGA ACCAATGAAG ACGTGCCAGG AGCACCGCCT 
CGGAAATTGG AAGTGGAGGC TTTGAACTCC ACCGCTATCC GTGTGACATG GAAACCTCCT 
CTTTCTGGAA AGCAGCATGG CCAGATCCGG GGATATCAGG TCATATACTC CCGGTTGGAG 
AACGGGGAAC CACGGGGACA TCCGAATATA ATGGATGTCG CTCTGCCAGA AGCACAGTGG 
AAAATTGAAG AGTCTACGGA ATATGAGGCT GTGATCGCTG GACTTGCCTC TGAAACCTCT 
TACTCAGTGA CTGTGGCTGC GTACACAACC AAAGGTGATG GAGCTCGCAG CAAAGCCAAA 
GTGGTCACCA CCACCGGAGC AGTGCCTGGC AAACCCACTA TGATCATCAG CACCACCGTT 
GGCAACACGG CCCTTATCCA GTGGCAGCCT CCTAAAGACA TGGTGGGTGA GCTGATGGGC 
TACCGGCTGC GCTACAAACG TCTGGAAGAG GAAAACTATG AGATACGCGA ATTCAGTCGC 
ACCGACGACC ACCACACCGT CACTGACCTG CACAAGGGCG CCACCTACAG CTTCCACCTG 
AGCGCCAGAA ACCGCGCTGG ACTCGGAGAG GAGTACGTTA AAGACATCGG CACTCCTGAG 
GACGTGCCCA GCGGGTTTCC TCTTAACCTG CAGGTCGTAG GCTTGACTAC CTCTACCACC 
CGACTGACCT GGGAGCCTCC AGCGTTGTCT GAACGTAACG GACGGATCAT ACATTACATT 
GTGGTTTACC GAGACATCAA CAGCAAACAA AACAGCACCA ACCGAACCTC CGACACACAG 
ATGACCATCC AAGGGCTCCG ACCTGACACC ACCTATGATA TCCGTGTGCA GGCGTTCACT 
AGCAAGGGTG GAGGTCCCAT AAGTCCCAGT ATCCAGAGCA GGACCATGTC TACGTCACCT 
GCATTTGCCA CAAGTTTTGG TGTCAAAGCT GTGATGAAAA CCTCTGTCCT TCTCACCTGG 
GAGGTGCCAG AAAATTATAA ATCACAAGTG CCTTTTAAGA TCCTCTATAA CCAGCAGAGT 
GTGGAAGTCC AGGGGAATCT GAAGAGGAAG CTGATCACAC GCCTGCAGCC GGACACTGAT 
TACTCGTTTG TGCTGATGAG CCGGGGAAAC AGCGCCGGTG GCCTACAACA GCAGGTCTCC 
ATCCGCACTG CACCAGACCT GTTCAAAACT AAACCTGTCC TGTACACGGC AGACCAGACC 
AACAATGGCA AACTCACCAT CAACCTGCCC AAAGTGCCCA CCTCTGCTCC TGTCAGGTGT 
TATTACATTG TGGTCGTGCC CGTCTCTCAG ACATCATCTC GTCAGTGGAT TAATCCCGAT 
GAGATGGAGC TGGATGAGCT CCTGGAGTCT AGTGAGGGAG CTCATCTCAG GAGACGTCGA 
CGGCACACAG ACACCGTAAG ACCGTACATC GCCGCAAAGC TATCCACGCT CCCAGAAACC 
TTCACTCTGG GCGATGAGCT AGACTACAGC GGCTTCATCA ACCGGCCTCT TCCCAACAAC 
CAACACTTCC AGTGTTTTGT GATGGCTGAA CTCAAGGACC AATACCCTGT TACTGCCAAT 
GAGAAGCAGC GGACCTTCTC GGCTAGCCCA TACTCAGACC CCATCATAGT GCAGGGTGAA 
AACCAAATGC AGCGCAGTGT GGACCAACCG GAGATGCTTT GGGTCATGGG CCCTGTGCTC 
GCTGTGGTGC TCATCATCAT CATAGTCATC GCCATCCTGC TCTTCAAAAG CAAACAGGAG 
AGGAAGAGGG CTTCCCCCCT ACCAAAAGAC GATCATTCGG GTGGTGTGAA GGATTGTCTT 
CTGGCCAGCT CATCCGATCC TGTCGAAATG AGGAGACTCA ACTACCAGAC ACCAGGAATG 
AGGGAACATC CTCCCATATC CGTCTCCGAA CTGGCAGACC ACATCGAGCG CCTCAAGGCC 
AACGATGCCC TCCGGTTTTC CCAAGAGTAT GAGTCTATTG ATCCAGGCCA GCAGTTCACA 
TGGGAGAACT CCAACTTGGA GGTCAACAAG CCAAAGAACC GTTATGCCAA CGTTATTGCT 
TACGACCACT CGAGAGTTGT TCTCACCCCT GTGGACGGTG TACCAGGGAG TGACTACATT 
AATTCCAACT ACATTGATGG CTACCGTAAG CAGAATGCCT ACATTGCCAC ACAAGGTCCT 
CTGCCTGAGA CTCTGAGCGA CTTCTGGAGG ATGATTTGGG AGCAACGAGC CAGTACCATC 
GTCATGATGA CCAGACTGGA AGAGAAGTCC AGAGTCAAGT GTGACCAATA CTGGCCCATT 
CGTGGCACAG AGACTTATGG AATGATTCAA GTGACCATGC TTGATGCTGT TGAGCTGGCA 
ACCTACAGTG TGCGCACCTT TGCCCTTTAC AAGAATGGCT CTAGTGAGAA GCGCGAGGTC 
AGGCAGTTTC AGTTCATGGC TTGGCCGGAC CACGGAGTGC CTGAATATCC CACCCCCATC 
CTAGCCTTCC TGCGCAGGGT CAAAGCCTGC AACCCTCCTG ATGCTGGACC CATGGTAGTG 
CACTGCAGTG CTGGAGTTGG CCGTACAGGC TGCTTCATAG TCATCGATGC GATGTTGGAG 
CGCATGAAGC ACGAGAAGAC CGTAGACATT TATGGCCACG TCACGTGCAT GAGATCCCAG 
AGGAACTATA TGGTGCAGAC GGAGGACCAG TACATCTTTA TCCACGAGGC CCTTCTGGAG 
GCCGCCACCT GTGGAAACAC AGAGGTCCCT GCCCGAAACC TCTACGCCCA CATCCAGAAG 
CTCACACAGC CATCGGCTGG AGAGACCGTC ACAGCCATGG AGCTGGAGTT TAAGAGACTC 
GCCAACTCCA AAGCCCACAC CTCAAGATTC ATCAGCGCCA ACCTGCCCTG CAACAAGTTC 
AAGAACCGGC TGGTCAATAT CATGCCTTTC GAGTCCACAC GGGTGTGCCT GCAGCCAATC 
CGAGGAGTGG AGGGGTCCGA CTACATCAAT GCCAGCTGCA TTGACGGATA CAGACAGCAG 
AAGGCCTATA TTGCCACTCA GGGTCCTCTA GCGGAGACCA CCGAGGACTT CTGGAGGATG 
TTATGGGAGC ACAATTCCAC TATTGTAGTG ATGCTGACCA AACTCAGAGA GATGGGACGG 
GAGAAGTGTC ATCAGTACTG GCCCGCCGAG CGCTCAGCCC GCTATCAGTA CTTCGTAGTG 
GATCCCATGG CCGAATACAA CATGCCCCAG TACATCCTGC GAGAGTTCAA AGTCACAGAT 
GCCAGGGACG GTCAATCCAG AACCATTCGC CAGTTCCAGT TCACTGATTG GCCAGAGCAG 
GGAGTGCCAA AAACCGGTGA GGGGTTCATC GATTTCATTG GGCAAGTTCA TAAAACCAAG 
GAGCAGTTTG GGCAGGATGG ACCAATCACT GTGCACTGCA GTGCTGGAGT GGGAAGAACA 
GGTGTTTTCA TCACTCTCAG TATTGTCCTG GAGAGGATGA GATATGAGGG GGTGGTGGAT 
CTGTTCCAGA CGGTTAAAAC CCTGCGCACA CAAAGGCCTG CCATGGTGCA GACAGAGGAC 
CAGTACCAGC TGTGCTACAG AGCCGCTCTG GAGTACCTGG GGAGCTTTGA TCACTATGCA 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296736.1
CDS	422..6187
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVIREDPRGVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298461.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298461.3

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTCATTCGA GAAGACCCTC GAGGAGTACG ACGAGTTCCA 
CCCCGTTTCT CCATCCCACC TACCAATCAC GAGGTGATGC CAGGGGGCAG TGTAAACCTC 
ACCTGCGTGG CAGTAGGTGC CCCGATGCCC TACGTCAAAT GGATGACTGG TGAGGTGGAG 
CTCACCAAGG ATGAGGAGAT GCCTGTGGGA CGCAACGTCT TGGAGCTCAC CAACATCCGT 
CAGTCGACCA ACTACACCTG CGTGGCTATA TCATCCCTAG GTATGATCGA GGCAACGGCA 
CAAGTGTCTG TCAAAGCTCT CCCAAAGCCT CCTACATCCC TGACTGTTAC GGAAACCACA 
GCTACGAGTG TTACACTCAC ATGGGACTCT GGAAACCCTG AACCTGTGTC TTACTACATA 
ATTCAATACC GGGCCAAGAC TTCAGACACC AACTTCCAAG AAGTGGACGG TGTGGCCACC 
ACACGTTACA GCATCGGCGG TCTCAGCCCA TATTCAGAGT ATGAGTTTCG AGTAATGGCA 
GTCAACAACA TTGGTCGTGG GCCACCTAGT GAACCCGTTG AAACACGAAC AGGTGAACAG 
GCACCTTCCT CCCCACCGCT GCATGTTCAA GCACGAATGC TGAGTGCCAG CACCATGTTG 
GTTCAGTGGG AACCACCAGA GGAACCCAAT GGTCAGATCA GAGGCTATCG GATCTACTAC 
ACATCTGACC TGGATGCACC TCTTAGCGCC TGGCAGAAAC ACAACACAGA CGATAGCCGT 
CTCACCACTA TCTCCAGCCT GACCACCGGC ATCACCTACA GCCTACGTGT GCTGGGCTTC 
ACATCTGTAG GGGATGGACC ACCATCTGAT GTGCTACAGG TCAAGACACA GCAAGGAGTT 
CCTGCTCAGC CATCCAGCTT CGAAGCTGAA GCAGAGCTAG ATACAAGGAT CCTACTAACT 
TGGCTGTGGC CCGTGCAGGA CCCCATCATC AAATACGAGC TGCAATATTG GGAGGCTGAC 
TCAGACAACA AGATCCATGT CACCTTTGAC CCAGCTGGCT CGTACGCTGT TGAAGGTCTG 
AAACCAGACA CTTTGTACAA ATTCTCTCTG GCAGCCCGCT CTGAAATGGG ACAAGGAGTC 
TTCACTCACC CCATTGAAGC CAGGACGGCC CAGTCTGCCC CCTCTGCCCC CCCTCAAGAC 
GTGCACCTGC TCAGTCTGAG CTCCACCAGC ATCAAGGTGA GTTGGGTGGC ACCACCCGCC 
GCTAGCCGCC ACGGCGCAAT AGTGCGCTAC ACAGTCAGCT ACCAGGCAGT GAATGGAGAA 
GACACAGAGC GCCATGAGGT GCCGGACATT GGAGCGGATG CCTCCAGCTG TGTGTTGGAA 
GGTCTAGAGA AGTGGACGGA GTATCAGGTG TGGGTGAGAG CGCACACTGA TGTGGGCCCC 
GGCCCCGAGA GCACGGCTGT GCGTATCCGA ACCAATGAAG ACGTGCCAGG AGCACCGCCT 
CGGAAATTGG AAGTGGAGGC TTTGAACTCC ACCGCTATCC GTGTGACATG GAAACCTCCT 
CTTTCTGGAA AGCAGCATGG CCAGATCCGG GGATATCAGG TCATATACTC CCGGTTGGAG 
AACGGGGAAC CACGGGGACA TCCGAATATA ATGGATGTCG CTCTGCCAGA AGCACAGTGG 
AAAATTGAAG AGTCTACGGA ATATGAGGCT GTGATCGCTG GACTTGCCTC TGAAACCTCT 
TACTCAGTGA CTGTGGCTGC GTACACAACC AAAGGTGATG GAGCTCGCAG CAAAGCCAAA 
GTGGTCACCA CCACCGGAGC AGTGCCTGGC AAACCCACTA TGATCATCAG CACCACCGTT 
GGCAACACGG CCCTTATCCA GTGGCAGCCT CCTAAAGACA TGGTGGGTGA GCTGATGGGC 
TACCGGCTGC GCTACAAACG TCTGGAAGAG GAAAACTATG AGATACGCGA ATTCAGTCGC 
ACCGACGACC ACCACACCGT CACTGACCTG CACAAGGGCG CCACCTACAG CTTCCACCTG 
AGCGCCAGAA ACCGCGCTGG ACTCGGAGAG GAGTACGTTA AAGACATCGG CACTCCTGAG 
GACGTGCCCA GCGGGTTTCC TCTTAACCTG CAGGTCGTAG GCTTGACTAC CTCTACCACC 
CGACTGACCT GGGAGCCTCC AGCGTTGTCT GAACGTAACG GACGGATCAT ACATTACATT 
GTGGTTTACC GAGACATCAA CAGCAAACAA AACAGCACCA ACCGAACCTC CGACACACAG 
ATGACCATCC AAGGGCTCCG ACCTGACACC ACCTATGATA TCCGTGTGCA GGCGTTCACT 
AGCAAGGGTG GAGGTCCCAT AAGTCCCAGT ATCCAGAGCA GGACCATGTC TACGTCACCT 
GCATTTGCCA CAAGTTTTGG TGTCAAAGCT GTGATGAAAA CCTCTGTCCT TCTCACCTGG 
GAGGTGCCAG AAAATTATAA ATCACAAGTG CCTTTTAAGA TCCTCTATAA CCAGCAGAGT 
GTGGAAGTCC AGGGGAATCT GAAGAGGAAG CTGATCACAC GCCTGCAGCC GGACACTGAT 
TACTCGTTTG TGCTGATGAG CCGGGGAAAC AGCGCCGGTG GCCTACAACA GCAGGTCTCC 
ATCCGCACTG CACCAGACCT GTTCAAAACT AAACCTGTCC TGTACACGGC AGACCAGACC 
AACAATGGCA AACTCACCAT CAACCTGCCC AAAGTGCCCA CCTCTGCTCC TGTCAGGTGT 
TATTACATTG TGGTCGTGCC CGTCTCTCAG ACATCATCTC GTCAGTGGAT TAATCCCGAT 
GAGATGGAGC TGGATGAGCT CCTGGAGTCT AGTGAGGGAG CTCATCTCAG GAGACGTCGA 
CGGCACACAG ACACCGTAAG ACCGTACATC GCCGCAAAGC TATCCACGCT CCCAGAAACC 
TTCACTCTGG GCGATGAGCT AGACTACAGC GGCTTCATCA ACCGGCCTCT TCCCAACAAC 
CAACACTTCC AGTGTTTTGT GATGGCTGAA CTCAAGGACC AATACCCTGT TACTGCCAAT 
GAGAAGCAGC GGACCTTCTC GGCTAGCCCA TACTCAGACC CCATCATAGT GCAGGGTGAA 
AACCAAATGC AGCGCAGTGT GGACCAACCG GAGATGCTTT GGGTCATGGG CCCTGTGCTC 
GCTGTGGTGC TCATCATCAT CATAGTCATC GCCATCCTGC TCTTCAAAAG CAAACAGGAG 
AGGAAGAGGG CTTCCCCCCT ACCAAAAGAC GATCATTCGG GTGGTGTGAA GGATTGTCTT 
CTGGCCAGCT CATCCGATCC TGTCGAAATG AGGAGACTCA ACTACCAGAC ACCAGGAATG 
AGGGAACATC CTCCCATATC CGTCTCCGAA CTGGCAGACC ACATCGAGCG CCTCAAGGCC 
AACGATGCCC TCCGGTTTTC CCAAGAGTAT GAGTCTATTG ATCCAGGCCA GCAGTTCACA 
TGGGAGAACT CCAACTTGGA GGTCAACAAG CCAAAGAACC GTTATGCCAA CGTTATTGCT 
TACGACCACT CGAGAGTTGT TCTCACCCCT GTGGACGGTG TACCAGGGAG TGACTACATT 
AATTCCAACT ACATTGATGG CTACCGTAAG CAGAATGCCT ACATTGCCAC ACAAGGTCCT 
CTGCCTGAGA CTCTGAGCGA CTTCTGGAGG ATGATTTGGG AGCAACGAGC CAGTACCATC 
GTCATGATGA CCAGACTGGA AGAGAAGTCC AGAGTCAAGT GTGACCAATA CTGGCCCATT 
CGTGGCACAG AGACTTATGG AATGATTCAA GTGACCATGC TTGATGCTGT TGAGCTGGCA 
ACCTACAGTG TGCGCACCTT TGCCCTTTAC AAGAATGGCT CTAGTGAGAA GCGCGAGGTC 
AGGCAGTTTC AGTTCATGGC TTGGCCGGAC CACGGAGTGC CTGAATATCC CACCCCCATC 
CTAGCCTTCC TGCGCAGGGT CAAAGCCTGC AACCCTCCTG ATGCTGGACC CATGGTAGTG 
CACTGCAGTG CTGGAGTTGG CCGTACAGGC TGCTTCATAG TCATCGATGC GATGTTGGAG 
CGCATGAAGC ACGAGAAGAC CGTAGACATT TATGGCCACG TCACGTGCAT GAGATCCCAG 
AGGAACTATA TGGTGCAGAC GGAGGACCAG TACATCTTTA TCCACGAGGC CCTTCTGGAG 
GCCGCCACCT GTGGAAACAC AGAGGTCCCT GCCCGAAACC TCTACGCCCA CATCCAGAAG 
CTCACACAGC CATCGGCTGG AGAGACCGTC ACAGCCATGG AGCTGGAGTT TAAGAGACTC 
GCCAACTCCA AAGCCCACAC CTCAAGATTC ATCAGCGCCA ACCTGCCCTG CAACAAGTTC 
AAGAACCGGC TGGTCAATAT CATGCCTTTC GAGTCCACAC GGGTGTGCCT GCAGCCAATC 
CGAGGAGTGG AGGGGTCCGA CTACATCAAT GCCAGCTGCA TTGACGGATA CAGACAGCAG 
AAGGCCTATA TTGCCACTCA GGGTCCTCTA GCGGAGACCA CCGAGGACTT CTGGAGGATG 
TTATGGGAGC ACAATTCCAC TATTGTAGTG ATGCTGACCA AACTCAGAGA GATGGGACGG 
GAGAAGTGTC ATCAGTACTG GCCCGCCGAG CGCTCAGCCC GCTATCAGTA CTTCGTAGTG 
GATCCCATGG CCGAATACAA CATGCCCCAG TACATCCTGC GAGAGTTCAA AGTCACAGAT 
GCCAGGGACG GTCAATCCAG AACCATTCGC CAGTTCCAGT TCACTGATTG GCCAGAGCAG 
GGAGTGCCAA AAACCGGTGA GGGGTTCATC GATTTCATTG GGCAAGTTCA TAAAACCAAG 
GAGCAGTTTG GGCAGGATGG ACCAATCACT GTGCACTGCA GTGCTGGAGT GGGAAGAACA 
GGTGTTTTCA TCACTCTCAG TATTGTCCTG GAGAGGATGA GATATGAGGG GGTGGTGGAT 
CTGTTCCAGA CGGTTAAAAC CCTGCGCACA CAAAGGCCTG CCATGGTGCA GACAGAGGAC 
CAGTACCAGC TGTGCTACAG AGCCGCTCTG GAGTACCTGG GGAGCTTTGA TCACTATGCA 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298461.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296736.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X5, mRNA.

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CloneID	ODa47416
Clone ID Related Accession (Same CDS sequence)	XM_009298461.3 , XM_009298461.2
Accession Version	XM_009298461.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTCATTCGA GAAGACCCTC GAGGAGTACG ACGAGTTCCA 
CCCCGTTTCT CCATCCCACC TACCAATCAC GAGGTGATGC CAGGGGGCAG TGTAAACCTC 
ACCTGCGTGG CAGTAGGTGC CCCGATGCCC TACGTCAAAT GGATGACTGG TGAGGTGGAG 
CTCACCAAGG ATGAGGAGAT GCCTGTGGGA CGCAACGTCT TGGAGCTCAC CAACATCCGT 
CAGTCGACCA ACTACACCTG CGTGGCTATA TCATCCCTAG GTATGATCGA GGCAACGGCA 
CAAGTGTCTG TCAAAGCTCT CCCAAAGCCT CCTACATCCC TGACTGTTAC GGAAACCACA 
GCTACGAGTG TTACACTCAC ATGGGACTCT GGAAACCCTG AACCTGTGTC TTACTACATA 
ATTCAATACC GGGCCAAGAC TTCAGACACC AACTTCCAAG AAGTGGACGG TGTGGCCACC 
ACACGTTACA GCATCGGCGG TCTCAGCCCA TATTCAGAGT ATGAGTTTCG AGTAATGGCA 
GTCAACAACA TTGGTCGTGG GCCACCTAGT GAACCCGTTG AAACACGAAC AGGTGAACAG 
GCACCTTCCT CCCCACCGCT GCATGTTCAA GCACGAATGC TGAGTGCCAG CACCATGTTG 
GTTCAGTGGG AACCACCAGA GGAACCCAAT GGTCAGATCA GAGGCTATCG GATCTACTAC 
ACATCTGACC TGGATGCACC TCTTAGCGCC TGGCAGAAAC ACAACACAGA CGATAGCCGT 
CTCACCACTA TCTCCAGCCT GACCACCGGC ATCACCTACA GCCTACGTGT GCTGGGCTTC 
ACATCTGTAG GGGATGGACC ACCATCTGAT GTGCTACAGG TCAAGACACA GCAAGGAGTT 
CCTGCTCAGC CATCCAGCTT CGAAGCTGAA GCAGAGCTAG ATACAAGGAT CCTACTAACT 
TGGCTGTGGC CCGTGCAGGA CCCCATCATC AAATACGAGC TGCAATATTG GGAGGCTGAC 
TCAGACAACA AGATCCATGT CACCTTTGAC CCAGCTGGCT CGTACGCTGT TGAAGGTCTG 
AAACCAGACA CTTTGTACAA ATTCTCTCTG GCAGCCCGCT CTGAAATGGG ACAAGGAGTC 
TTCACTCACC CCATTGAAGC CAGGACGGCC CAGTCTGCCC CCTCTGCCCC CCCTCAAGAC 
GTGCACCTGC TCAGTCTGAG CTCCACCAGC ATCAAGGTGA GTTGGGTGGC ACCACCCGCC 
GCTAGCCGCC ACGGCGCAAT AGTGCGCTAC ACAGTCAGCT ACCAGGCAGT GAATGGAGAA 
GACACAGAGC GCCATGAGGT GCCGGACATT GGAGCGGATG CCTCCAGCTG TGTGTTGGAA 
GGTCTAGAGA AGTGGACGGA GTATCAGGTG TGGGTGAGAG CGCACACTGA TGTGGGCCCC 
GGCCCCGAGA GCACGGCTGT GCGTATCCGA ACCAATGAAG ACGTGCCAGG AGCACCGCCT 
CGGAAATTGG AAGTGGAGGC TTTGAACTCC ACCGCTATCC GTGTGACATG GAAACCTCCT 
CTTTCTGGAA AGCAGCATGG CCAGATCCGG GGATATCAGG TCATATACTC CCGGTTGGAG 
AACGGGGAAC CACGGGGACA TCCGAATATA ATGGATGTCG CTCTGCCAGA AGCACAGTGG 
AAAATTGAAG AGTCTACGGA ATATGAGGCT GTGATCGCTG GACTTGCCTC TGAAACCTCT 
TACTCAGTGA CTGTGGCTGC GTACACAACC AAAGGTGATG GAGCTCGCAG CAAAGCCAAA 
GTGGTCACCA CCACCGGAGC AGTGCCTGGC AAACCCACTA TGATCATCAG CACCACCGTT 
GGCAACACGG CCCTTATCCA GTGGCAGCCT CCTAAAGACA TGGTGGGTGA GCTGATGGGC 
TACCGGCTGC GCTACAAACG TCTGGAAGAG GAAAACTATG AGATACGCGA ATTCAGTCGC 
ACCGACGACC ACCACACCGT CACTGACCTG CACAAGGGCG CCACCTACAG CTTCCACCTG 
AGCGCCAGAA ACCGCGCTGG ACTCGGAGAG GAGTACGTTA AAGACATCGG CACTCCTGAG 
GACGTGCCCA GCGGGTTTCC TCTTAACCTG CAGGTCGTAG GCTTGACTAC CTCTACCACC 
CGACTGACCT GGGAGCCTCC AGCGTTGTCT GAACGTAACG GACGGATCAT ACATTACATT 
GTGGTTTACC GAGACATCAA CAGCAAACAA AACAGCACCA ACCGAACCTC CGACACACAG 
ATGACCATCC AAGGGCTCCG ACCTGACACC ACCTATGATA TCCGTGTGCA GGCGTTCACT 
AGCAAGGGTG GAGGTCCCAT AAGTCCCAGT ATCCAGAGCA GGACCATGTC TACGTCACCT 
GCATTTGCCA CAAGTTTTGG TGTCAAAGCT GTGATGAAAA CCTCTGTCCT TCTCACCTGG 
GAGGTGCCAG AAAATTATAA ATCACAAGTG CCTTTTAAGA TCCTCTATAA CCAGCAGAGT 
GTGGAAGTCC AGGGGAATCT GAAGAGGAAG CTGATCACAC GCCTGCAGCC GGACACTGAT 
TACTCGTTTG TGCTGATGAG CCGGGGAAAC AGCGCCGGTG GCCTACAACA GCAGGTCTCC 
ATCCGCACTG CACCAGACCT GTTCAAAACT AAACCTGTCC TGTACACGGC AGACCAGACC 
AACAATGGCA AACTCACCAT CAACCTGCCC AAAGTGCCCA CCTCTGCTCC TGTCAGGTGT 
TATTACATTG TGGTCGTGCC CGTCTCTCAG ACATCATCTC GTCAGTGGAT TAATCCCGAT 
GAGATGGAGC TGGATGAGCT CCTGGAGTCT AGTGAGGGAG CTCATCTCAG GAGACGTCGA 
CGGCACACAG ACACCGTAAG ACCGTACATC GCCGCAAAGC TATCCACGCT CCCAGAAACC 
TTCACTCTGG GCGATGAGCT AGACTACAGC GGCTTCATCA ACCGGCCTCT TCCCAACAAC 
CAACACTTCC AGTGTTTTGT GATGGCTGAA CTCAAGGACC AATACCCTGT TACTGCCAAT 
GAGAAGCAGC GGACCTTCTC GGCTAGCCCA TACTCAGACC CCATCATAGT GCAGGGTGAA 
AACCAAATGC AGCGCAGTGT GGACCAACCG GAGATGCTTT GGGTCATGGG CCCTGTGCTC 
GCTGTGGTGC TCATCATCAT CATAGTCATC GCCATCCTGC TCTTCAAAAG CAAACAGGAG 
AGGAAGAGGG CTTCCCCCCT ACCAAAAGAC GATCATTCGG GTGGTGTGAA GGATTGTCTT 
CTGGCCAGCT CATCCGATCC TGTCGAAATG AGGAGACTCA ACTACCAGAC ACCAGGAATG 
AGGGAACATC CTCCCATATC CGTCTCCGAA CTGGCAGACC ACATCGAGCG CCTCAAGGCC 
AACGATGCCC TCCGGTTTTC CCAAGAGTAT GAGTCTATTG ATCCAGGCCA GCAGTTCACA 
TGGGAGAACT CCAACTTGGA GGTCAACAAG CCAAAGAACC GTTATGCCAA CGTTATTGCT 
TACGACCACT CGAGAGTTGT TCTCACCCCT GTGGACGGTG TACCAGGGAG TGACTACATT 
AATTCCAACT ACATTGATGG CTACCGTAAG CAGAATGCCT ACATTGCCAC ACAAGGTCCT 
CTGCCTGAGA CTCTGAGCGA CTTCTGGAGG ATGATTTGGG AGCAACGAGC CAGTACCATC 
GTCATGATGA CCAGACTGGA AGAGAAGTCC AGAGTCAAGT GTGACCAATA CTGGCCCATT 
CGTGGCACAG AGACTTATGG AATGATTCAA GTGACCATGC TTGATGCTGT TGAGCTGGCA 
ACCTACAGTG TGCGCACCTT TGCCCTTTAC AAGAATGGCT CTAGTGAGAA GCGCGAGGTC 
AGGCAGTTTC AGTTCATGGC TTGGCCGGAC CACGGAGTGC CTGAATATCC CACCCCCATC 
CTAGCCTTCC TGCGCAGGGT CAAAGCCTGC AACCCTCCTG ATGCTGGACC CATGGTAGTG 
CACTGCAGTG CTGGAGTTGG CCGTACAGGC TGCTTCATAG TCATCGATGC GATGTTGGAG 
CGCATGAAGC ACGAGAAGAC CGTAGACATT TATGGCCACG TCACGTGCAT GAGATCCCAG 
AGGAACTATA TGGTGCAGAC GGAGGACCAG TACATCTTTA TCCACGAGGC CCTTCTGGAG 
GCCGCCACCT GTGGAAACAC AGAGGTCCCT GCCCGAAACC TCTACGCCCA CATCCAGAAG 
CTCACACAGC CATCGGCTGG AGAGACCGTC ACAGCCATGG AGCTGGAGTT TAAGAGACTC 
GCCAACTCCA AAGCCCACAC CTCAAGATTC ATCAGCGCCA ACCTGCCCTG CAACAAGTTC 
AAGAACCGGC TGGTCAATAT CATGCCTTTC GAGTCCACAC GGGTGTGCCT GCAGCCAATC 
CGAGGAGTGG AGGGGTCCGA CTACATCAAT GCCAGCTGCA TTGACGGATA CAGACAGCAG 
AAGGCCTATA TTGCCACTCA GGGTCCTCTA GCGGAGACCA CCGAGGACTT CTGGAGGATG 
TTATGGGAGC ACAATTCCAC TATTGTAGTG ATGCTGACCA AACTCAGAGA GATGGGACGG 
GAGAAGTGTC ATCAGTACTG GCCCGCCGAG CGCTCAGCCC GCTATCAGTA CTTCGTAGTG 
GATCCCATGG CCGAATACAA CATGCCCCAG TACATCCTGC GAGAGTTCAA AGTCACAGAT 
GCCAGGGACG GTCAATCCAG AACCATTCGC CAGTTCCAGT TCACTGATTG GCCAGAGCAG 
GGAGTGCCAA AAACCGGTGA GGGGTTCATC GATTTCATTG GGCAAGTTCA TAAAACCAAG 
GAGCAGTTTG GGCAGGATGG ACCAATCACT GTGCACTGCA GTGCTGGAGT GGGAAGAACA 
GGTGTTTTCA TCACTCTCAG TATTGTCCTG GAGAGGATGA GATATGAGGG GGTGGTGGAT 
CTGTTCCAGA CGGTTAAAAC CCTGCGCACA CAAAGGCCTG CCATGGTGCA GACAGAGGAC 
CAGTACCAGC TGTGCTACAG AGCCGCTCTG GAGTACCTGG GGAGCTTTGA TCACTATGCA 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296736.1
CDS	426..6191
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVIREDPRGVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298461.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298461.2

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTCATTCGA GAAGACCCTC GAGGAGTACG ACGAGTTCCA 
CCCCGTTTCT CCATCCCACC TACCAATCAC GAGGTGATGC CAGGGGGCAG TGTAAACCTC 
ACCTGCGTGG CAGTAGGTGC CCCGATGCCC TACGTCAAAT GGATGACTGG TGAGGTGGAG 
CTCACCAAGG ATGAGGAGAT GCCTGTGGGA CGCAACGTCT TGGAGCTCAC CAACATCCGT 
CAGTCGACCA ACTACACCTG CGTGGCTATA TCATCCCTAG GTATGATCGA GGCAACGGCA 
CAAGTGTCTG TCAAAGCTCT CCCAAAGCCT CCTACATCCC TGACTGTTAC GGAAACCACA 
GCTACGAGTG TTACACTCAC ATGGGACTCT GGAAACCCTG AACCTGTGTC TTACTACATA 
ATTCAATACC GGGCCAAGAC TTCAGACACC AACTTCCAAG AAGTGGACGG TGTGGCCACC 
ACACGTTACA GCATCGGCGG TCTCAGCCCA TATTCAGAGT ATGAGTTTCG AGTAATGGCA 
GTCAACAACA TTGGTCGTGG GCCACCTAGT GAACCCGTTG AAACACGAAC AGGTGAACAG 
GCACCTTCCT CCCCACCGCT GCATGTTCAA GCACGAATGC TGAGTGCCAG CACCATGTTG 
GTTCAGTGGG AACCACCAGA GGAACCCAAT GGTCAGATCA GAGGCTATCG GATCTACTAC 
ACATCTGACC TGGATGCACC TCTTAGCGCC TGGCAGAAAC ACAACACAGA CGATAGCCGT 
CTCACCACTA TCTCCAGCCT GACCACCGGC ATCACCTACA GCCTACGTGT GCTGGGCTTC 
ACATCTGTAG GGGATGGACC ACCATCTGAT GTGCTACAGG TCAAGACACA GCAAGGAGTT 
CCTGCTCAGC CATCCAGCTT CGAAGCTGAA GCAGAGCTAG ATACAAGGAT CCTACTAACT 
TGGCTGTGGC CCGTGCAGGA CCCCATCATC AAATACGAGC TGCAATATTG GGAGGCTGAC 
TCAGACAACA AGATCCATGT CACCTTTGAC CCAGCTGGCT CGTACGCTGT TGAAGGTCTG 
AAACCAGACA CTTTGTACAA ATTCTCTCTG GCAGCCCGCT CTGAAATGGG ACAAGGAGTC 
TTCACTCACC CCATTGAAGC CAGGACGGCC CAGTCTGCCC CCTCTGCCCC CCCTCAAGAC 
GTGCACCTGC TCAGTCTGAG CTCCACCAGC ATCAAGGTGA GTTGGGTGGC ACCACCCGCC 
GCTAGCCGCC ACGGCGCAAT AGTGCGCTAC ACAGTCAGCT ACCAGGCAGT GAATGGAGAA 
GACACAGAGC GCCATGAGGT GCCGGACATT GGAGCGGATG CCTCCAGCTG TGTGTTGGAA 
GGTCTAGAGA AGTGGACGGA GTATCAGGTG TGGGTGAGAG CGCACACTGA TGTGGGCCCC 
GGCCCCGAGA GCACGGCTGT GCGTATCCGA ACCAATGAAG ACGTGCCAGG AGCACCGCCT 
CGGAAATTGG AAGTGGAGGC TTTGAACTCC ACCGCTATCC GTGTGACATG GAAACCTCCT 
CTTTCTGGAA AGCAGCATGG CCAGATCCGG GGATATCAGG TCATATACTC CCGGTTGGAG 
AACGGGGAAC CACGGGGACA TCCGAATATA ATGGATGTCG CTCTGCCAGA AGCACAGTGG 
AAAATTGAAG AGTCTACGGA ATATGAGGCT GTGATCGCTG GACTTGCCTC TGAAACCTCT 
TACTCAGTGA CTGTGGCTGC GTACACAACC AAAGGTGATG GAGCTCGCAG CAAAGCCAAA 
GTGGTCACCA CCACCGGAGC AGTGCCTGGC AAACCCACTA TGATCATCAG CACCACCGTT 
GGCAACACGG CCCTTATCCA GTGGCAGCCT CCTAAAGACA TGGTGGGTGA GCTGATGGGC 
TACCGGCTGC GCTACAAACG TCTGGAAGAG GAAAACTATG AGATACGCGA ATTCAGTCGC 
ACCGACGACC ACCACACCGT CACTGACCTG CACAAGGGCG CCACCTACAG CTTCCACCTG 
AGCGCCAGAA ACCGCGCTGG ACTCGGAGAG GAGTACGTTA AAGACATCGG CACTCCTGAG 
GACGTGCCCA GCGGGTTTCC TCTTAACCTG CAGGTCGTAG GCTTGACTAC CTCTACCACC 
CGACTGACCT GGGAGCCTCC AGCGTTGTCT GAACGTAACG GACGGATCAT ACATTACATT 
GTGGTTTACC GAGACATCAA CAGCAAACAA AACAGCACCA ACCGAACCTC CGACACACAG 
ATGACCATCC AAGGGCTCCG ACCTGACACC ACCTATGATA TCCGTGTGCA GGCGTTCACT 
AGCAAGGGTG GAGGTCCCAT AAGTCCCAGT ATCCAGAGCA GGACCATGTC TACGTCACCT 
GCATTTGCCA CAAGTTTTGG TGTCAAAGCT GTGATGAAAA CCTCTGTCCT TCTCACCTGG 
GAGGTGCCAG AAAATTATAA ATCACAAGTG CCTTTTAAGA TCCTCTATAA CCAGCAGAGT 
GTGGAAGTCC AGGGGAATCT GAAGAGGAAG CTGATCACAC GCCTGCAGCC GGACACTGAT 
TACTCGTTTG TGCTGATGAG CCGGGGAAAC AGCGCCGGTG GCCTACAACA GCAGGTCTCC 
ATCCGCACTG CACCAGACCT GTTCAAAACT AAACCTGTCC TGTACACGGC AGACCAGACC 
AACAATGGCA AACTCACCAT CAACCTGCCC AAAGTGCCCA CCTCTGCTCC TGTCAGGTGT 
TATTACATTG TGGTCGTGCC CGTCTCTCAG ACATCATCTC GTCAGTGGAT TAATCCCGAT 
GAGATGGAGC TGGATGAGCT CCTGGAGTCT AGTGAGGGAG CTCATCTCAG GAGACGTCGA 
CGGCACACAG ACACCGTAAG ACCGTACATC GCCGCAAAGC TATCCACGCT CCCAGAAACC 
TTCACTCTGG GCGATGAGCT AGACTACAGC GGCTTCATCA ACCGGCCTCT TCCCAACAAC 
CAACACTTCC AGTGTTTTGT GATGGCTGAA CTCAAGGACC AATACCCTGT TACTGCCAAT 
GAGAAGCAGC GGACCTTCTC GGCTAGCCCA TACTCAGACC CCATCATAGT GCAGGGTGAA 
AACCAAATGC AGCGCAGTGT GGACCAACCG GAGATGCTTT GGGTCATGGG CCCTGTGCTC 
GCTGTGGTGC TCATCATCAT CATAGTCATC GCCATCCTGC TCTTCAAAAG CAAACAGGAG 
AGGAAGAGGG CTTCCCCCCT ACCAAAAGAC GATCATTCGG GTGGTGTGAA GGATTGTCTT 
CTGGCCAGCT CATCCGATCC TGTCGAAATG AGGAGACTCA ACTACCAGAC ACCAGGAATG 
AGGGAACATC CTCCCATATC CGTCTCCGAA CTGGCAGACC ACATCGAGCG CCTCAAGGCC 
AACGATGCCC TCCGGTTTTC CCAAGAGTAT GAGTCTATTG ATCCAGGCCA GCAGTTCACA 
TGGGAGAACT CCAACTTGGA GGTCAACAAG CCAAAGAACC GTTATGCCAA CGTTATTGCT 
TACGACCACT CGAGAGTTGT TCTCACCCCT GTGGACGGTG TACCAGGGAG TGACTACATT 
AATTCCAACT ACATTGATGG CTACCGTAAG CAGAATGCCT ACATTGCCAC ACAAGGTCCT 
CTGCCTGAGA CTCTGAGCGA CTTCTGGAGG ATGATTTGGG AGCAACGAGC CAGTACCATC 
GTCATGATGA CCAGACTGGA AGAGAAGTCC AGAGTCAAGT GTGACCAATA CTGGCCCATT 
CGTGGCACAG AGACTTATGG AATGATTCAA GTGACCATGC TTGATGCTGT TGAGCTGGCA 
ACCTACAGTG TGCGCACCTT TGCCCTTTAC AAGAATGGCT CTAGTGAGAA GCGCGAGGTC 
AGGCAGTTTC AGTTCATGGC TTGGCCGGAC CACGGAGTGC CTGAATATCC CACCCCCATC 
CTAGCCTTCC TGCGCAGGGT CAAAGCCTGC AACCCTCCTG ATGCTGGACC CATGGTAGTG 
CACTGCAGTG CTGGAGTTGG CCGTACAGGC TGCTTCATAG TCATCGATGC GATGTTGGAG 
CGCATGAAGC ACGAGAAGAC CGTAGACATT TATGGCCACG TCACGTGCAT GAGATCCCAG 
AGGAACTATA TGGTGCAGAC GGAGGACCAG TACATCTTTA TCCACGAGGC CCTTCTGGAG 
GCCGCCACCT GTGGAAACAC AGAGGTCCCT GCCCGAAACC TCTACGCCCA CATCCAGAAG 
CTCACACAGC CATCGGCTGG AGAGACCGTC ACAGCCATGG AGCTGGAGTT TAAGAGACTC 
GCCAACTCCA AAGCCCACAC CTCAAGATTC ATCAGCGCCA ACCTGCCCTG CAACAAGTTC 
AAGAACCGGC TGGTCAATAT CATGCCTTTC GAGTCCACAC GGGTGTGCCT GCAGCCAATC 
CGAGGAGTGG AGGGGTCCGA CTACATCAAT GCCAGCTGCA TTGACGGATA CAGACAGCAG 
AAGGCCTATA TTGCCACTCA GGGTCCTCTA GCGGAGACCA CCGAGGACTT CTGGAGGATG 
TTATGGGAGC ACAATTCCAC TATTGTAGTG ATGCTGACCA AACTCAGAGA GATGGGACGG 
GAGAAGTGTC ATCAGTACTG GCCCGCCGAG CGCTCAGCCC GCTATCAGTA CTTCGTAGTG 
GATCCCATGG CCGAATACAA CATGCCCCAG TACATCCTGC GAGAGTTCAA AGTCACAGAT 
GCCAGGGACG GTCAATCCAG AACCATTCGC CAGTTCCAGT TCACTGATTG GCCAGAGCAG 
GGAGTGCCAA AAACCGGTGA GGGGTTCATC GATTTCATTG GGCAAGTTCA TAAAACCAAG 
GAGCAGTTTG GGCAGGATGG ACCAATCACT GTGCACTGCA GTGCTGGAGT GGGAAGAACA 
GGTGTTTTCA TCACTCTCAG TATTGTCCTG GAGAGGATGA GATATGAGGG GGTGGTGGAT 
CTGTTCCAGA CGGTTAAAAC CCTGCGCACA CAAAGGCCTG CCATGGTGCA GACAGAGGAC 
CAGTACCAGC TGTGCTACAG AGCCGCTCTG GAGTACCTGG GGAGCTTTGA TCACTATGCA 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298462.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296737.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X6, mRNA.

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CloneID	ODa47417
Clone ID Related Accession (Same CDS sequence)	XM_009298462.2 , XM_009298462.3
Accession Version	XM_009298462.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298462.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TACGACGAGT TCCACCCCGT 
TTCTCCATCC CACCTACCAA TCACGAGGTG ATGCCAGGGG GCAGTGTAAA CCTCACCTGC 
GTGGCAGTAG GTGCCCCGAT GCCCTACGTC AAATGGATGA CTGGTGAGGT GGAGCTCACC 
AAGGATGAGG AGATGCCTGT GGGACGCAAC GTCTTGGAGC TCACCAACAT CCGTCAGTCG 
ACCAACTACA CCTGCGTGGC TATATCATCC CTAGGTATGA TCGAGGCAAC GGCACAAGTG 
TCTGTCAAAG CTCTCCCAAA GCCTCCTACA TCCCTGACTG TTACGGAAAC CACAGCTACG 
AGTGTTACAC TCACATGGGA CTCTGGAAAC CCTGAACCTG TGTCTTACTA CATAATTCAA 
TACCGGGCCA AGACTTCAGA CACCAACTTC CAAGAAGTGG ACGGTGTGGC CACCACACGT 
TACAGCATCG GCGGTCTCAG CCCATATTCA GAGTATGAGT TTCGAGTAAT GGCAGTCAAC 
AACATTGGTC GTGGGCCACC TAGTGAACCC GTTGAAACAC GAACAGGTGA ACAGGCACCT 
TCCTCCCCAC CGCTGCATGT TCAAGCACGA ATGCTGAGTG CCAGCACCAT GTTGGTTCAG 
TGGGAACCAC CAGAGGAACC CAATGGTCAG ATCAGAGGCT ATCGGATCTA CTACACATCT 
GACCTGGATG CACCTCTTAG CGCCTGGCAG AAACACAACA CAGACGATAG CCGTCTCACC 
ACTATCTCCA GCCTGACCAC CGGCATCACC TACAGCCTAC GTGTGCTGGG CTTCACATCT 
GTAGGGGATG GACCACCATC TGATGTGCTA CAGGTCAAGA CACAGCAAGG AGTTCCTGCT 
CAGCCATCCA GCTTCGAAGC TGAAGCAGAG CTAGATACAA GGATCCTACT AACTTGGCTG 
TGGCCCGTGC AGGACCCCAT CATCAAATAC GAGCTGCAAT ATTGGGAGGC TGACTCAGAC 
AACAAGATCC ATGTCACCTT TGACCCAGCT GGCTCGTACG CTGTTGAAGG TCTGAAACCA 
GACACTTTGT ACAAATTCTC TCTGGCAGCC CGCTCTGAAA TGGGACAAGG AGTCTTCACT 
CACCCCATTG AAGCCAGGAC GGCCCAGTCT GCCCCCTCTG CCCCCCCTCA AGACGTGCAC 
CTGCTCAGTC TGAGCTCCAC CAGCATCAAG GTGAGTTGGG TGGCACCACC CGCCGCTAGC 
CGCCACGGCG CAATAGTGCG CTACACAGTC AGCTACCAGG CAGTGAATGG AGAAGACACA 
GAGCGCCATG AGGTGCCGGA CATTGGAGCG GATGCCTCCA GCTGTGTGTT GGAAGGTCTA 
GAGAAGTGGA CGGAGTATCA GGTGTGGGTG AGAGCGCACA CTGATGTGGG CCCCGGCCCC 
GAGAGCACGG CTGTGCGTAT CCGAACCAAT GAAGACGTGC CAGGAGCACC GCCTCGGAAA 
TTGGAAGTGG AGGCTTTGAA CTCCACCGCT ATCCGTGTGA CATGGAAACC TCCTCTTTCT 
GGAAAGCAGC ATGGCCAGAT CCGGGGATAT CAGGTCATAT ACTCCCGGTT GGAGAACGGG 
GAACCACGGG GACATCCGAA TATAATGGAT GTCGCTCTGC CAGAAGCACA GTGGAAAATT 
GAAGAGTCTA CGGAATATGA GGCTGTGATC GCTGGACTTG CCTCTGAAAC CTCTTACTCA 
GTGACTGTGG CTGCGTACAC AACCAAAGGT GATGGAGCTC GCAGCAAAGC CAAAGTGGTC 
ACCACCACCG GAGCAGTGCC TGGCAAACCC ACTATGATCA TCAGCACCAC CGTTGGCAAC 
ACGGCCCTTA TCCAGTGGCA GCCTCCTAAA GACATGGTGG GTGAGCTGAT GGGCTACCGG 
CTGCGCTACA AACGTCTGGA AGAGGAAAAC TATGAGATAC GCGAATTCAG TCGCACCGAC 
GACCACCACA CCGTCACTGA CCTGCACAAG GGCGCCACCT ACAGCTTCCA CCTGAGCGCC 
AGAAACCGCG CTGGACTCGG AGAGGAGTAC GTTAAAGACA TCGGCACTCC TGAGGACGTG 
CCCAGCGGGT TTCCTCTTAA CCTGCAGGTC GTAGGCTTGA CTACCTCTAC CACCCGACTG 
ACCTGGGAGC CTCCAGCGTT GTCTGAACGT AACGGACGGA TCATACATTA CATTGTGGTT 
TACCGAGACA TCAACAGCAA ACAAAACAGC ACCAACCGAA CCTCCGACAC ACAGATGACC 
ATCCAAGGGC TCCGACCTGA CACCACCTAT GATATCCGTG TGCAGGCGTT CACTAGCAAG 
GGTGGAGGTC CCATAAGTCC CAGTATCCAG AGCAGGACCA TGTCTACGTC ACCTGCATTT 
GCCACAAGTT TTGGTGTCAA AGCTGTGATG AAAACCTCTG TCCTTCTCAC CTGGGAGGTG 
CCAGAAAATT ATAAATCACA AGTGCCTTTT AAGATCCTCT ATAACCAGCA GAGTGTGGAA 
GTCCAGGGGA ATCTGAAGAG GAAGCTGATC ACACGCCTGC AGCCGGACAC TGATTACTCG 
TTTGTGCTGA TGAGCCGGGG AAACAGCGCC GGTGGCCTAC AACAGCAGGT CTCCATCCGC 
ACTGCACCAG ACCTGTTCAA AACTAAACCT GTCCTGTACA CGGCAGACCA GACCAACAAT 
GGCAAACTCA CCATCAACCT GCCCAAAGTG CCCACCTCTG CTCCTGTCAG GTGTTATTAC 
ATTGTGGTCG TGCCCGTCTC TCAGACATCA TCTCGTCAGT GGATTAATCC CGATGAGATG 
GAGCTGGATG AGCTCCTGGA GTCTAGTGAG GGAGCTCATC TCAGGAGACG TCGACGGCAC 
ACAGACACCG TAAGACCGTA CATCGCCGCA AAGCTATCCA CGCTCCCAGA AACCTTCACT 
CTGGGCGATG AGCTAGACTA CAGCGGCTTC ATCAACCGGC CTCTTCCCAA CAACCAACAC 
TTCCAGTGTT TTGTGATGGC TGAACTCAAG GACCAATACC CTGTTACTGC CAATGAGAAG 
CAGCGGACCT TCTCGGCTAG CCCATACTCA GACCCCATCA TAGTGCAGGG TGAAAACCAA 
ATGCAGCGCA GTGTGGACCA ACCGGAGATG CTTTGGGTCA TGGGCCCTGT GCTCGCTGTG 
GTGCTCATCA TCATCATAGT CATCGCCATC CTGCTCTTCA AAAGCAAACA GGAGAGGAAG 
AGGGCTTCCC CCCTACCAAA AGACGATCAT TCGGGTGGTG TGAAGGATTG TCTTCTGGCC 
AGCTCATCCG ATCCTGTCGA AATGAGGAGA CTCAACTACC AGACACCAGG AATGAGGGAA 
CATCCTCCCA TATCCGTCTC CGAACTGGCA GACCACATCG AGCGCCTCAA GGCCAACGAT 
GCCCTCCGGT TTTCCCAAGA GTATGAGTCT ATTGATCCAG GCCAGCAGTT CACATGGGAG 
AACTCCAACT TGGAGGTCAA CAAGCCAAAG AACCGTTATG CCAACGTTAT TGCTTACGAC 
CACTCGAGAG TTGTTCTCAC CCCTGTGGAC GGTGTACCAG GGAGTGACTA CATTAATTCC 
AACTACATTG ATGGCTACCG TAAGCAGAAT GCCTACATTG CCACACAAGG TCCTCTGCCT 
GAGACTCTGA GCGACTTCTG GAGGATGATT TGGGAGCAAC GAGCCAGTAC CATCGTCATG 
ATGACCAGAC TGGAAGAGAA GTCCAGAGTC AAGTGTGACC AATACTGGCC CATTCGTGGC 
ACAGAGACTT ATGGAATGAT TCAAGTGACC ATGCTTGATG CTGTTGAGCT GGCAACCTAC 
AGTGTGCGCA CCTTTGCCCT TTACAAGAAT GGCTCTAGTG AGAAGCGCGA GGTCAGGCAG 
TTTCAGTTCA TGGCTTGGCC GGACCACGGA GTGCCTGAAT ATCCCACCCC CATCCTAGCC 
TTCCTGCGCA GGGTCAAAGC CTGCAACCCT CCTGATGCTG GACCCATGGT AGTGCACTGC 
AGTGCTGGAG TTGGCCGTAC AGGCTGCTTC ATAGTCATCG ATGCGATGTT GGAGCGCATG 
AAGCACGAGA AGACCGTAGA CATTTATGGC CACGTCACGT GCATGAGATC CCAGAGGAAC 
TATATGGTGC AGACGGAGGA CCAGTACATC TTTATCCACG AGGCCCTTCT GGAGGCCGCC 
ACCTGTGGAA ACACAGAGGT CCCTGCCCGA AACCTCTACG CCCACATCCA GAAGCTCACA 
CAGCCATCGG CTGGAGAGAC CGTCACAGCC ATGGAGCTGG AGTTTAAGAG ACTCGCCAAC 
TCCAAAGCCC ACACCTCAAG ATTCATCAGC GCCAACCTGC CCTGCAACAA GTTCAAGAAC 
CGGCTGGTCA ATATCATGCC TTTCGAGTCC ACACGGGTGT GCCTGCAGCC AATCCGAGGA 
GTGGAGGGGT CCGACTACAT CAATGCCAGC TGCATTGACG GATACAGACA GCAGAAGGCC 
TATATTGCCA CTCAGGGTCC TCTAGCGGAG ACCACCGAGG ACTTCTGGAG GATGTTATGG 
GAGCACAATT CCACTATTGT AGTGATGCTG ACCAAACTCA GAGAGATGGG ACGGGAGAAG 
TGTCATCAGT ACTGGCCCGC CGAGCGCTCA GCCCGCTATC AGTACTTCGT AGTGGATCCC 
ATGGCCGAAT ACAACATGCC CCAGTACATC CTGCGAGAGT TCAAAGTCAC AGATGCCAGG 
GACGGTCAAT CCAGAACCAT TCGCCAGTTC CAGTTCACTG ATTGGCCAGA GCAGGGAGTG 
CCAAAAACCG GTGAGGGGTT CATCGATTTC ATTGGGCAAG TTCATAAAAC CAAGGAGCAG 
TTTGGGCAGG ATGGACCAAT CACTGTGCAC TGCAGTGCTG GAGTGGGAAG AACAGGTGTT 
TTCATCACTC TCAGTATTGT CCTGGAGAGG ATGAGATATG AGGGGGTGGT GGATCTGTTC 
CAGACGGTTA AAACCCTGCG CACACAAAGG CCTGCCATGG TGCAGACAGA GGACCAGTAC 
CAGCTGTGCT ACAGAGCCGC TCTGGAGTAC CTGGGGAGCT TTGATCACTA TGCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296737.1
CDS	426..6185
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298462.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298462.2

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TACGACGAGT TCCACCCCGT 
TTCTCCATCC CACCTACCAA TCACGAGGTG ATGCCAGGGG GCAGTGTAAA CCTCACCTGC 
GTGGCAGTAG GTGCCCCGAT GCCCTACGTC AAATGGATGA CTGGTGAGGT GGAGCTCACC 
AAGGATGAGG AGATGCCTGT GGGACGCAAC GTCTTGGAGC TCACCAACAT CCGTCAGTCG 
ACCAACTACA CCTGCGTGGC TATATCATCC CTAGGTATGA TCGAGGCAAC GGCACAAGTG 
TCTGTCAAAG CTCTCCCAAA GCCTCCTACA TCCCTGACTG TTACGGAAAC CACAGCTACG 
AGTGTTACAC TCACATGGGA CTCTGGAAAC CCTGAACCTG TGTCTTACTA CATAATTCAA 
TACCGGGCCA AGACTTCAGA CACCAACTTC CAAGAAGTGG ACGGTGTGGC CACCACACGT 
TACAGCATCG GCGGTCTCAG CCCATATTCA GAGTATGAGT TTCGAGTAAT GGCAGTCAAC 
AACATTGGTC GTGGGCCACC TAGTGAACCC GTTGAAACAC GAACAGGTGA ACAGGCACCT 
TCCTCCCCAC CGCTGCATGT TCAAGCACGA ATGCTGAGTG CCAGCACCAT GTTGGTTCAG 
TGGGAACCAC CAGAGGAACC CAATGGTCAG ATCAGAGGCT ATCGGATCTA CTACACATCT 
GACCTGGATG CACCTCTTAG CGCCTGGCAG AAACACAACA CAGACGATAG CCGTCTCACC 
ACTATCTCCA GCCTGACCAC CGGCATCACC TACAGCCTAC GTGTGCTGGG CTTCACATCT 
GTAGGGGATG GACCACCATC TGATGTGCTA CAGGTCAAGA CACAGCAAGG AGTTCCTGCT 
CAGCCATCCA GCTTCGAAGC TGAAGCAGAG CTAGATACAA GGATCCTACT AACTTGGCTG 
TGGCCCGTGC AGGACCCCAT CATCAAATAC GAGCTGCAAT ATTGGGAGGC TGACTCAGAC 
AACAAGATCC ATGTCACCTT TGACCCAGCT GGCTCGTACG CTGTTGAAGG TCTGAAACCA 
GACACTTTGT ACAAATTCTC TCTGGCAGCC CGCTCTGAAA TGGGACAAGG AGTCTTCACT 
CACCCCATTG AAGCCAGGAC GGCCCAGTCT GCCCCCTCTG CCCCCCCTCA AGACGTGCAC 
CTGCTCAGTC TGAGCTCCAC CAGCATCAAG GTGAGTTGGG TGGCACCACC CGCCGCTAGC 
CGCCACGGCG CAATAGTGCG CTACACAGTC AGCTACCAGG CAGTGAATGG AGAAGACACA 
GAGCGCCATG AGGTGCCGGA CATTGGAGCG GATGCCTCCA GCTGTGTGTT GGAAGGTCTA 
GAGAAGTGGA CGGAGTATCA GGTGTGGGTG AGAGCGCACA CTGATGTGGG CCCCGGCCCC 
GAGAGCACGG CTGTGCGTAT CCGAACCAAT GAAGACGTGC CAGGAGCACC GCCTCGGAAA 
TTGGAAGTGG AGGCTTTGAA CTCCACCGCT ATCCGTGTGA CATGGAAACC TCCTCTTTCT 
GGAAAGCAGC ATGGCCAGAT CCGGGGATAT CAGGTCATAT ACTCCCGGTT GGAGAACGGG 
GAACCACGGG GACATCCGAA TATAATGGAT GTCGCTCTGC CAGAAGCACA GTGGAAAATT 
GAAGAGTCTA CGGAATATGA GGCTGTGATC GCTGGACTTG CCTCTGAAAC CTCTTACTCA 
GTGACTGTGG CTGCGTACAC AACCAAAGGT GATGGAGCTC GCAGCAAAGC CAAAGTGGTC 
ACCACCACCG GAGCAGTGCC TGGCAAACCC ACTATGATCA TCAGCACCAC CGTTGGCAAC 
ACGGCCCTTA TCCAGTGGCA GCCTCCTAAA GACATGGTGG GTGAGCTGAT GGGCTACCGG 
CTGCGCTACA AACGTCTGGA AGAGGAAAAC TATGAGATAC GCGAATTCAG TCGCACCGAC 
GACCACCACA CCGTCACTGA CCTGCACAAG GGCGCCACCT ACAGCTTCCA CCTGAGCGCC 
AGAAACCGCG CTGGACTCGG AGAGGAGTAC GTTAAAGACA TCGGCACTCC TGAGGACGTG 
CCCAGCGGGT TTCCTCTTAA CCTGCAGGTC GTAGGCTTGA CTACCTCTAC CACCCGACTG 
ACCTGGGAGC CTCCAGCGTT GTCTGAACGT AACGGACGGA TCATACATTA CATTGTGGTT 
TACCGAGACA TCAACAGCAA ACAAAACAGC ACCAACCGAA CCTCCGACAC ACAGATGACC 
ATCCAAGGGC TCCGACCTGA CACCACCTAT GATATCCGTG TGCAGGCGTT CACTAGCAAG 
GGTGGAGGTC CCATAAGTCC CAGTATCCAG AGCAGGACCA TGTCTACGTC ACCTGCATTT 
GCCACAAGTT TTGGTGTCAA AGCTGTGATG AAAACCTCTG TCCTTCTCAC CTGGGAGGTG 
CCAGAAAATT ATAAATCACA AGTGCCTTTT AAGATCCTCT ATAACCAGCA GAGTGTGGAA 
GTCCAGGGGA ATCTGAAGAG GAAGCTGATC ACACGCCTGC AGCCGGACAC TGATTACTCG 
TTTGTGCTGA TGAGCCGGGG AAACAGCGCC GGTGGCCTAC AACAGCAGGT CTCCATCCGC 
ACTGCACCAG ACCTGTTCAA AACTAAACCT GTCCTGTACA CGGCAGACCA GACCAACAAT 
GGCAAACTCA CCATCAACCT GCCCAAAGTG CCCACCTCTG CTCCTGTCAG GTGTTATTAC 
ATTGTGGTCG TGCCCGTCTC TCAGACATCA TCTCGTCAGT GGATTAATCC CGATGAGATG 
GAGCTGGATG AGCTCCTGGA GTCTAGTGAG GGAGCTCATC TCAGGAGACG TCGACGGCAC 
ACAGACACCG TAAGACCGTA CATCGCCGCA AAGCTATCCA CGCTCCCAGA AACCTTCACT 
CTGGGCGATG AGCTAGACTA CAGCGGCTTC ATCAACCGGC CTCTTCCCAA CAACCAACAC 
TTCCAGTGTT TTGTGATGGC TGAACTCAAG GACCAATACC CTGTTACTGC CAATGAGAAG 
CAGCGGACCT TCTCGGCTAG CCCATACTCA GACCCCATCA TAGTGCAGGG TGAAAACCAA 
ATGCAGCGCA GTGTGGACCA ACCGGAGATG CTTTGGGTCA TGGGCCCTGT GCTCGCTGTG 
GTGCTCATCA TCATCATAGT CATCGCCATC CTGCTCTTCA AAAGCAAACA GGAGAGGAAG 
AGGGCTTCCC CCCTACCAAA AGACGATCAT TCGGGTGGTG TGAAGGATTG TCTTCTGGCC 
AGCTCATCCG ATCCTGTCGA AATGAGGAGA CTCAACTACC AGACACCAGG AATGAGGGAA 
CATCCTCCCA TATCCGTCTC CGAACTGGCA GACCACATCG AGCGCCTCAA GGCCAACGAT 
GCCCTCCGGT TTTCCCAAGA GTATGAGTCT ATTGATCCAG GCCAGCAGTT CACATGGGAG 
AACTCCAACT TGGAGGTCAA CAAGCCAAAG AACCGTTATG CCAACGTTAT TGCTTACGAC 
CACTCGAGAG TTGTTCTCAC CCCTGTGGAC GGTGTACCAG GGAGTGACTA CATTAATTCC 
AACTACATTG ATGGCTACCG TAAGCAGAAT GCCTACATTG CCACACAAGG TCCTCTGCCT 
GAGACTCTGA GCGACTTCTG GAGGATGATT TGGGAGCAAC GAGCCAGTAC CATCGTCATG 
ATGACCAGAC TGGAAGAGAA GTCCAGAGTC AAGTGTGACC AATACTGGCC CATTCGTGGC 
ACAGAGACTT ATGGAATGAT TCAAGTGACC ATGCTTGATG CTGTTGAGCT GGCAACCTAC 
AGTGTGCGCA CCTTTGCCCT TTACAAGAAT GGCTCTAGTG AGAAGCGCGA GGTCAGGCAG 
TTTCAGTTCA TGGCTTGGCC GGACCACGGA GTGCCTGAAT ATCCCACCCC CATCCTAGCC 
TTCCTGCGCA GGGTCAAAGC CTGCAACCCT CCTGATGCTG GACCCATGGT AGTGCACTGC 
AGTGCTGGAG TTGGCCGTAC AGGCTGCTTC ATAGTCATCG ATGCGATGTT GGAGCGCATG 
AAGCACGAGA AGACCGTAGA CATTTATGGC CACGTCACGT GCATGAGATC CCAGAGGAAC 
TATATGGTGC AGACGGAGGA CCAGTACATC TTTATCCACG AGGCCCTTCT GGAGGCCGCC 
ACCTGTGGAA ACACAGAGGT CCCTGCCCGA AACCTCTACG CCCACATCCA GAAGCTCACA 
CAGCCATCGG CTGGAGAGAC CGTCACAGCC ATGGAGCTGG AGTTTAAGAG ACTCGCCAAC 
TCCAAAGCCC ACACCTCAAG ATTCATCAGC GCCAACCTGC CCTGCAACAA GTTCAAGAAC 
CGGCTGGTCA ATATCATGCC TTTCGAGTCC ACACGGGTGT GCCTGCAGCC AATCCGAGGA 
GTGGAGGGGT CCGACTACAT CAATGCCAGC TGCATTGACG GATACAGACA GCAGAAGGCC 
TATATTGCCA CTCAGGGTCC TCTAGCGGAG ACCACCGAGG ACTTCTGGAG GATGTTATGG 
GAGCACAATT CCACTATTGT AGTGATGCTG ACCAAACTCA GAGAGATGGG ACGGGAGAAG 
TGTCATCAGT ACTGGCCCGC CGAGCGCTCA GCCCGCTATC AGTACTTCGT AGTGGATCCC 
ATGGCCGAAT ACAACATGCC CCAGTACATC CTGCGAGAGT TCAAAGTCAC AGATGCCAGG 
GACGGTCAAT CCAGAACCAT TCGCCAGTTC CAGTTCACTG ATTGGCCAGA GCAGGGAGTG 
CCAAAAACCG GTGAGGGGTT CATCGATTTC ATTGGGCAAG TTCATAAAAC CAAGGAGCAG 
TTTGGGCAGG ATGGACCAAT CACTGTGCAC TGCAGTGCTG GAGTGGGAAG AACAGGTGTT 
TTCATCACTC TCAGTATTGT CCTGGAGAGG ATGAGATATG AGGGGGTGGT GGATCTGTTC 
CAGACGGTTA AAACCCTGCG CACACAAAGG CCTGCCATGG TGCAGACAGA GGACCAGTAC 
CAGCTGTGCT ACAGAGCCGC TCTGGAGTAC CTGGGGAGCT TTGATCACTA TGCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298462.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296737.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X7, mRNA.

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CloneID	ODa47417
Clone ID Related Accession (Same CDS sequence)	XM_009298462.2 , XM_009298462.3
Accession Version	XM_009298462.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298462.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TACGACGAGT TCCACCCCGT 
TTCTCCATCC CACCTACCAA TCACGAGGTG ATGCCAGGGG GCAGTGTAAA CCTCACCTGC 
GTGGCAGTAG GTGCCCCGAT GCCCTACGTC AAATGGATGA CTGGTGAGGT GGAGCTCACC 
AAGGATGAGG AGATGCCTGT GGGACGCAAC GTCTTGGAGC TCACCAACAT CCGTCAGTCG 
ACCAACTACA CCTGCGTGGC TATATCATCC CTAGGTATGA TCGAGGCAAC GGCACAAGTG 
TCTGTCAAAG CTCTCCCAAA GCCTCCTACA TCCCTGACTG TTACGGAAAC CACAGCTACG 
AGTGTTACAC TCACATGGGA CTCTGGAAAC CCTGAACCTG TGTCTTACTA CATAATTCAA 
TACCGGGCCA AGACTTCAGA CACCAACTTC CAAGAAGTGG ACGGTGTGGC CACCACACGT 
TACAGCATCG GCGGTCTCAG CCCATATTCA GAGTATGAGT TTCGAGTAAT GGCAGTCAAC 
AACATTGGTC GTGGGCCACC TAGTGAACCC GTTGAAACAC GAACAGGTGA ACAGGCACCT 
TCCTCCCCAC CGCTGCATGT TCAAGCACGA ATGCTGAGTG CCAGCACCAT GTTGGTTCAG 
TGGGAACCAC CAGAGGAACC CAATGGTCAG ATCAGAGGCT ATCGGATCTA CTACACATCT 
GACCTGGATG CACCTCTTAG CGCCTGGCAG AAACACAACA CAGACGATAG CCGTCTCACC 
ACTATCTCCA GCCTGACCAC CGGCATCACC TACAGCCTAC GTGTGCTGGG CTTCACATCT 
GTAGGGGATG GACCACCATC TGATGTGCTA CAGGTCAAGA CACAGCAAGG AGTTCCTGCT 
CAGCCATCCA GCTTCGAAGC TGAAGCAGAG CTAGATACAA GGATCCTACT AACTTGGCTG 
TGGCCCGTGC AGGACCCCAT CATCAAATAC GAGCTGCAAT ATTGGGAGGC TGACTCAGAC 
AACAAGATCC ATGTCACCTT TGACCCAGCT GGCTCGTACG CTGTTGAAGG TCTGAAACCA 
GACACTTTGT ACAAATTCTC TCTGGCAGCC CGCTCTGAAA TGGGACAAGG AGTCTTCACT 
CACCCCATTG AAGCCAGGAC GGCCCAGTCT GCCCCCTCTG CCCCCCCTCA AGACGTGCAC 
CTGCTCAGTC TGAGCTCCAC CAGCATCAAG GTGAGTTGGG TGGCACCACC CGCCGCTAGC 
CGCCACGGCG CAATAGTGCG CTACACAGTC AGCTACCAGG CAGTGAATGG AGAAGACACA 
GAGCGCCATG AGGTGCCGGA CATTGGAGCG GATGCCTCCA GCTGTGTGTT GGAAGGTCTA 
GAGAAGTGGA CGGAGTATCA GGTGTGGGTG AGAGCGCACA CTGATGTGGG CCCCGGCCCC 
GAGAGCACGG CTGTGCGTAT CCGAACCAAT GAAGACGTGC CAGGAGCACC GCCTCGGAAA 
TTGGAAGTGG AGGCTTTGAA CTCCACCGCT ATCCGTGTGA CATGGAAACC TCCTCTTTCT 
GGAAAGCAGC ATGGCCAGAT CCGGGGATAT CAGGTCATAT ACTCCCGGTT GGAGAACGGG 
GAACCACGGG GACATCCGAA TATAATGGAT GTCGCTCTGC CAGAAGCACA GTGGAAAATT 
GAAGAGTCTA CGGAATATGA GGCTGTGATC GCTGGACTTG CCTCTGAAAC CTCTTACTCA 
GTGACTGTGG CTGCGTACAC AACCAAAGGT GATGGAGCTC GCAGCAAAGC CAAAGTGGTC 
ACCACCACCG GAGCAGTGCC TGGCAAACCC ACTATGATCA TCAGCACCAC CGTTGGCAAC 
ACGGCCCTTA TCCAGTGGCA GCCTCCTAAA GACATGGTGG GTGAGCTGAT GGGCTACCGG 
CTGCGCTACA AACGTCTGGA AGAGGAAAAC TATGAGATAC GCGAATTCAG TCGCACCGAC 
GACCACCACA CCGTCACTGA CCTGCACAAG GGCGCCACCT ACAGCTTCCA CCTGAGCGCC 
AGAAACCGCG CTGGACTCGG AGAGGAGTAC GTTAAAGACA TCGGCACTCC TGAGGACGTG 
CCCAGCGGGT TTCCTCTTAA CCTGCAGGTC GTAGGCTTGA CTACCTCTAC CACCCGACTG 
ACCTGGGAGC CTCCAGCGTT GTCTGAACGT AACGGACGGA TCATACATTA CATTGTGGTT 
TACCGAGACA TCAACAGCAA ACAAAACAGC ACCAACCGAA CCTCCGACAC ACAGATGACC 
ATCCAAGGGC TCCGACCTGA CACCACCTAT GATATCCGTG TGCAGGCGTT CACTAGCAAG 
GGTGGAGGTC CCATAAGTCC CAGTATCCAG AGCAGGACCA TGTCTACGTC ACCTGCATTT 
GCCACAAGTT TTGGTGTCAA AGCTGTGATG AAAACCTCTG TCCTTCTCAC CTGGGAGGTG 
CCAGAAAATT ATAAATCACA AGTGCCTTTT AAGATCCTCT ATAACCAGCA GAGTGTGGAA 
GTCCAGGGGA ATCTGAAGAG GAAGCTGATC ACACGCCTGC AGCCGGACAC TGATTACTCG 
TTTGTGCTGA TGAGCCGGGG AAACAGCGCC GGTGGCCTAC AACAGCAGGT CTCCATCCGC 
ACTGCACCAG ACCTGTTCAA AACTAAACCT GTCCTGTACA CGGCAGACCA GACCAACAAT 
GGCAAACTCA CCATCAACCT GCCCAAAGTG CCCACCTCTG CTCCTGTCAG GTGTTATTAC 
ATTGTGGTCG TGCCCGTCTC TCAGACATCA TCTCGTCAGT GGATTAATCC CGATGAGATG 
GAGCTGGATG AGCTCCTGGA GTCTAGTGAG GGAGCTCATC TCAGGAGACG TCGACGGCAC 
ACAGACACCG TAAGACCGTA CATCGCCGCA AAGCTATCCA CGCTCCCAGA AACCTTCACT 
CTGGGCGATG AGCTAGACTA CAGCGGCTTC ATCAACCGGC CTCTTCCCAA CAACCAACAC 
TTCCAGTGTT TTGTGATGGC TGAACTCAAG GACCAATACC CTGTTACTGC CAATGAGAAG 
CAGCGGACCT TCTCGGCTAG CCCATACTCA GACCCCATCA TAGTGCAGGG TGAAAACCAA 
ATGCAGCGCA GTGTGGACCA ACCGGAGATG CTTTGGGTCA TGGGCCCTGT GCTCGCTGTG 
GTGCTCATCA TCATCATAGT CATCGCCATC CTGCTCTTCA AAAGCAAACA GGAGAGGAAG 
AGGGCTTCCC CCCTACCAAA AGACGATCAT TCGGGTGGTG TGAAGGATTG TCTTCTGGCC 
AGCTCATCCG ATCCTGTCGA AATGAGGAGA CTCAACTACC AGACACCAGG AATGAGGGAA 
CATCCTCCCA TATCCGTCTC CGAACTGGCA GACCACATCG AGCGCCTCAA GGCCAACGAT 
GCCCTCCGGT TTTCCCAAGA GTATGAGTCT ATTGATCCAG GCCAGCAGTT CACATGGGAG 
AACTCCAACT TGGAGGTCAA CAAGCCAAAG AACCGTTATG CCAACGTTAT TGCTTACGAC 
CACTCGAGAG TTGTTCTCAC CCCTGTGGAC GGTGTACCAG GGAGTGACTA CATTAATTCC 
AACTACATTG ATGGCTACCG TAAGCAGAAT GCCTACATTG CCACACAAGG TCCTCTGCCT 
GAGACTCTGA GCGACTTCTG GAGGATGATT TGGGAGCAAC GAGCCAGTAC CATCGTCATG 
ATGACCAGAC TGGAAGAGAA GTCCAGAGTC AAGTGTGACC AATACTGGCC CATTCGTGGC 
ACAGAGACTT ATGGAATGAT TCAAGTGACC ATGCTTGATG CTGTTGAGCT GGCAACCTAC 
AGTGTGCGCA CCTTTGCCCT TTACAAGAAT GGCTCTAGTG AGAAGCGCGA GGTCAGGCAG 
TTTCAGTTCA TGGCTTGGCC GGACCACGGA GTGCCTGAAT ATCCCACCCC CATCCTAGCC 
TTCCTGCGCA GGGTCAAAGC CTGCAACCCT CCTGATGCTG GACCCATGGT AGTGCACTGC 
AGTGCTGGAG TTGGCCGTAC AGGCTGCTTC ATAGTCATCG ATGCGATGTT GGAGCGCATG 
AAGCACGAGA AGACCGTAGA CATTTATGGC CACGTCACGT GCATGAGATC CCAGAGGAAC 
TATATGGTGC AGACGGAGGA CCAGTACATC TTTATCCACG AGGCCCTTCT GGAGGCCGCC 
ACCTGTGGAA ACACAGAGGT CCCTGCCCGA AACCTCTACG CCCACATCCA GAAGCTCACA 
CAGCCATCGG CTGGAGAGAC CGTCACAGCC ATGGAGCTGG AGTTTAAGAG ACTCGCCAAC 
TCCAAAGCCC ACACCTCAAG ATTCATCAGC GCCAACCTGC CCTGCAACAA GTTCAAGAAC 
CGGCTGGTCA ATATCATGCC TTTCGAGTCC ACACGGGTGT GCCTGCAGCC AATCCGAGGA 
GTGGAGGGGT CCGACTACAT CAATGCCAGC TGCATTGACG GATACAGACA GCAGAAGGCC 
TATATTGCCA CTCAGGGTCC TCTAGCGGAG ACCACCGAGG ACTTCTGGAG GATGTTATGG 
GAGCACAATT CCACTATTGT AGTGATGCTG ACCAAACTCA GAGAGATGGG ACGGGAGAAG 
TGTCATCAGT ACTGGCCCGC CGAGCGCTCA GCCCGCTATC AGTACTTCGT AGTGGATCCC 
ATGGCCGAAT ACAACATGCC CCAGTACATC CTGCGAGAGT TCAAAGTCAC AGATGCCAGG 
GACGGTCAAT CCAGAACCAT TCGCCAGTTC CAGTTCACTG ATTGGCCAGA GCAGGGAGTG 
CCAAAAACCG GTGAGGGGTT CATCGATTTC ATTGGGCAAG TTCATAAAAC CAAGGAGCAG 
TTTGGGCAGG ATGGACCAAT CACTGTGCAC TGCAGTGCTG GAGTGGGAAG AACAGGTGTT 
TTCATCACTC TCAGTATTGT CCTGGAGAGG ATGAGATATG AGGGGGTGGT GGATCTGTTC 
CAGACGGTTA AAACCCTGCG CACACAAAGG CCTGCCATGG TGCAGACAGA GGACCAGTAC 
CAGCTGTGCT ACAGAGCCGC TCTGGAGTAC CTGGGGAGCT TTGATCACTA TGCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296737.1
CDS	422..6181
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298462.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298462.3

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TACGACGAGT TCCACCCCGT 
TTCTCCATCC CACCTACCAA TCACGAGGTG ATGCCAGGGG GCAGTGTAAA CCTCACCTGC 
GTGGCAGTAG GTGCCCCGAT GCCCTACGTC AAATGGATGA CTGGTGAGGT GGAGCTCACC 
AAGGATGAGG AGATGCCTGT GGGACGCAAC GTCTTGGAGC TCACCAACAT CCGTCAGTCG 
ACCAACTACA CCTGCGTGGC TATATCATCC CTAGGTATGA TCGAGGCAAC GGCACAAGTG 
TCTGTCAAAG CTCTCCCAAA GCCTCCTACA TCCCTGACTG TTACGGAAAC CACAGCTACG 
AGTGTTACAC TCACATGGGA CTCTGGAAAC CCTGAACCTG TGTCTTACTA CATAATTCAA 
TACCGGGCCA AGACTTCAGA CACCAACTTC CAAGAAGTGG ACGGTGTGGC CACCACACGT 
TACAGCATCG GCGGTCTCAG CCCATATTCA GAGTATGAGT TTCGAGTAAT GGCAGTCAAC 
AACATTGGTC GTGGGCCACC TAGTGAACCC GTTGAAACAC GAACAGGTGA ACAGGCACCT 
TCCTCCCCAC CGCTGCATGT TCAAGCACGA ATGCTGAGTG CCAGCACCAT GTTGGTTCAG 
TGGGAACCAC CAGAGGAACC CAATGGTCAG ATCAGAGGCT ATCGGATCTA CTACACATCT 
GACCTGGATG CACCTCTTAG CGCCTGGCAG AAACACAACA CAGACGATAG CCGTCTCACC 
ACTATCTCCA GCCTGACCAC CGGCATCACC TACAGCCTAC GTGTGCTGGG CTTCACATCT 
GTAGGGGATG GACCACCATC TGATGTGCTA CAGGTCAAGA CACAGCAAGG AGTTCCTGCT 
CAGCCATCCA GCTTCGAAGC TGAAGCAGAG CTAGATACAA GGATCCTACT AACTTGGCTG 
TGGCCCGTGC AGGACCCCAT CATCAAATAC GAGCTGCAAT ATTGGGAGGC TGACTCAGAC 
AACAAGATCC ATGTCACCTT TGACCCAGCT GGCTCGTACG CTGTTGAAGG TCTGAAACCA 
GACACTTTGT ACAAATTCTC TCTGGCAGCC CGCTCTGAAA TGGGACAAGG AGTCTTCACT 
CACCCCATTG AAGCCAGGAC GGCCCAGTCT GCCCCCTCTG CCCCCCCTCA AGACGTGCAC 
CTGCTCAGTC TGAGCTCCAC CAGCATCAAG GTGAGTTGGG TGGCACCACC CGCCGCTAGC 
CGCCACGGCG CAATAGTGCG CTACACAGTC AGCTACCAGG CAGTGAATGG AGAAGACACA 
GAGCGCCATG AGGTGCCGGA CATTGGAGCG GATGCCTCCA GCTGTGTGTT GGAAGGTCTA 
GAGAAGTGGA CGGAGTATCA GGTGTGGGTG AGAGCGCACA CTGATGTGGG CCCCGGCCCC 
GAGAGCACGG CTGTGCGTAT CCGAACCAAT GAAGACGTGC CAGGAGCACC GCCTCGGAAA 
TTGGAAGTGG AGGCTTTGAA CTCCACCGCT ATCCGTGTGA CATGGAAACC TCCTCTTTCT 
GGAAAGCAGC ATGGCCAGAT CCGGGGATAT CAGGTCATAT ACTCCCGGTT GGAGAACGGG 
GAACCACGGG GACATCCGAA TATAATGGAT GTCGCTCTGC CAGAAGCACA GTGGAAAATT 
GAAGAGTCTA CGGAATATGA GGCTGTGATC GCTGGACTTG CCTCTGAAAC CTCTTACTCA 
GTGACTGTGG CTGCGTACAC AACCAAAGGT GATGGAGCTC GCAGCAAAGC CAAAGTGGTC 
ACCACCACCG GAGCAGTGCC TGGCAAACCC ACTATGATCA TCAGCACCAC CGTTGGCAAC 
ACGGCCCTTA TCCAGTGGCA GCCTCCTAAA GACATGGTGG GTGAGCTGAT GGGCTACCGG 
CTGCGCTACA AACGTCTGGA AGAGGAAAAC TATGAGATAC GCGAATTCAG TCGCACCGAC 
GACCACCACA CCGTCACTGA CCTGCACAAG GGCGCCACCT ACAGCTTCCA CCTGAGCGCC 
AGAAACCGCG CTGGACTCGG AGAGGAGTAC GTTAAAGACA TCGGCACTCC TGAGGACGTG 
CCCAGCGGGT TTCCTCTTAA CCTGCAGGTC GTAGGCTTGA CTACCTCTAC CACCCGACTG 
ACCTGGGAGC CTCCAGCGTT GTCTGAACGT AACGGACGGA TCATACATTA CATTGTGGTT 
TACCGAGACA TCAACAGCAA ACAAAACAGC ACCAACCGAA CCTCCGACAC ACAGATGACC 
ATCCAAGGGC TCCGACCTGA CACCACCTAT GATATCCGTG TGCAGGCGTT CACTAGCAAG 
GGTGGAGGTC CCATAAGTCC CAGTATCCAG AGCAGGACCA TGTCTACGTC ACCTGCATTT 
GCCACAAGTT TTGGTGTCAA AGCTGTGATG AAAACCTCTG TCCTTCTCAC CTGGGAGGTG 
CCAGAAAATT ATAAATCACA AGTGCCTTTT AAGATCCTCT ATAACCAGCA GAGTGTGGAA 
GTCCAGGGGA ATCTGAAGAG GAAGCTGATC ACACGCCTGC AGCCGGACAC TGATTACTCG 
TTTGTGCTGA TGAGCCGGGG AAACAGCGCC GGTGGCCTAC AACAGCAGGT CTCCATCCGC 
ACTGCACCAG ACCTGTTCAA AACTAAACCT GTCCTGTACA CGGCAGACCA GACCAACAAT 
GGCAAACTCA CCATCAACCT GCCCAAAGTG CCCACCTCTG CTCCTGTCAG GTGTTATTAC 
ATTGTGGTCG TGCCCGTCTC TCAGACATCA TCTCGTCAGT GGATTAATCC CGATGAGATG 
GAGCTGGATG AGCTCCTGGA GTCTAGTGAG GGAGCTCATC TCAGGAGACG TCGACGGCAC 
ACAGACACCG TAAGACCGTA CATCGCCGCA AAGCTATCCA CGCTCCCAGA AACCTTCACT 
CTGGGCGATG AGCTAGACTA CAGCGGCTTC ATCAACCGGC CTCTTCCCAA CAACCAACAC 
TTCCAGTGTT TTGTGATGGC TGAACTCAAG GACCAATACC CTGTTACTGC CAATGAGAAG 
CAGCGGACCT TCTCGGCTAG CCCATACTCA GACCCCATCA TAGTGCAGGG TGAAAACCAA 
ATGCAGCGCA GTGTGGACCA ACCGGAGATG CTTTGGGTCA TGGGCCCTGT GCTCGCTGTG 
GTGCTCATCA TCATCATAGT CATCGCCATC CTGCTCTTCA AAAGCAAACA GGAGAGGAAG 
AGGGCTTCCC CCCTACCAAA AGACGATCAT TCGGGTGGTG TGAAGGATTG TCTTCTGGCC 
AGCTCATCCG ATCCTGTCGA AATGAGGAGA CTCAACTACC AGACACCAGG AATGAGGGAA 
CATCCTCCCA TATCCGTCTC CGAACTGGCA GACCACATCG AGCGCCTCAA GGCCAACGAT 
GCCCTCCGGT TTTCCCAAGA GTATGAGTCT ATTGATCCAG GCCAGCAGTT CACATGGGAG 
AACTCCAACT TGGAGGTCAA CAAGCCAAAG AACCGTTATG CCAACGTTAT TGCTTACGAC 
CACTCGAGAG TTGTTCTCAC CCCTGTGGAC GGTGTACCAG GGAGTGACTA CATTAATTCC 
AACTACATTG ATGGCTACCG TAAGCAGAAT GCCTACATTG CCACACAAGG TCCTCTGCCT 
GAGACTCTGA GCGACTTCTG GAGGATGATT TGGGAGCAAC GAGCCAGTAC CATCGTCATG 
ATGACCAGAC TGGAAGAGAA GTCCAGAGTC AAGTGTGACC AATACTGGCC CATTCGTGGC 
ACAGAGACTT ATGGAATGAT TCAAGTGACC ATGCTTGATG CTGTTGAGCT GGCAACCTAC 
AGTGTGCGCA CCTTTGCCCT TTACAAGAAT GGCTCTAGTG AGAAGCGCGA GGTCAGGCAG 
TTTCAGTTCA TGGCTTGGCC GGACCACGGA GTGCCTGAAT ATCCCACCCC CATCCTAGCC 
TTCCTGCGCA GGGTCAAAGC CTGCAACCCT CCTGATGCTG GACCCATGGT AGTGCACTGC 
AGTGCTGGAG TTGGCCGTAC AGGCTGCTTC ATAGTCATCG ATGCGATGTT GGAGCGCATG 
AAGCACGAGA AGACCGTAGA CATTTATGGC CACGTCACGT GCATGAGATC CCAGAGGAAC 
TATATGGTGC AGACGGAGGA CCAGTACATC TTTATCCACG AGGCCCTTCT GGAGGCCGCC 
ACCTGTGGAA ACACAGAGGT CCCTGCCCGA AACCTCTACG CCCACATCCA GAAGCTCACA 
CAGCCATCGG CTGGAGAGAC CGTCACAGCC ATGGAGCTGG AGTTTAAGAG ACTCGCCAAC 
TCCAAAGCCC ACACCTCAAG ATTCATCAGC GCCAACCTGC CCTGCAACAA GTTCAAGAAC 
CGGCTGGTCA ATATCATGCC TTTCGAGTCC ACACGGGTGT GCCTGCAGCC AATCCGAGGA 
GTGGAGGGGT CCGACTACAT CAATGCCAGC TGCATTGACG GATACAGACA GCAGAAGGCC 
TATATTGCCA CTCAGGGTCC TCTAGCGGAG ACCACCGAGG ACTTCTGGAG GATGTTATGG 
GAGCACAATT CCACTATTGT AGTGATGCTG ACCAAACTCA GAGAGATGGG ACGGGAGAAG 
TGTCATCAGT ACTGGCCCGC CGAGCGCTCA GCCCGCTATC AGTACTTCGT AGTGGATCCC 
ATGGCCGAAT ACAACATGCC CCAGTACATC CTGCGAGAGT TCAAAGTCAC AGATGCCAGG 
GACGGTCAAT CCAGAACCAT TCGCCAGTTC CAGTTCACTG ATTGGCCAGA GCAGGGAGTG 
CCAAAAACCG GTGAGGGGTT CATCGATTTC ATTGGGCAAG TTCATAAAAC CAAGGAGCAG 
TTTGGGCAGG ATGGACCAAT CACTGTGCAC TGCAGTGCTG GAGTGGGAAG AACAGGTGTT 
TTCATCACTC TCAGTATTGT CCTGGAGAGG ATGAGATATG AGGGGGTGGT GGATCTGTTC 
CAGACGGTTA AAACCCTGCG CACACAAAGG CCTGCCATGG TGCAGACAGA GGACCAGTAC 
CAGCTGTGCT ACAGAGCCGC TCTGGAGTAC CTGGGGAGCT TTGATCACTA TGCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298463.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296738.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X8, mRNA.

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CloneID	ODa47418
Clone ID Related Accession (Same CDS sequence)	XM_009298463.3 , XM_009298463.2
Accession Version	XM_009298463.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298463.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGGAGGC TGTGATCGCT GGACTTGCCT CTGAAACCTC TTACTCAGTG 
ACTGTGGCTG CGTACACAAC CAAAGGTGAT GGAGCTCGCA GCAAAGCCAA AGTGGTCACC 
ACCACCGGAG CAGTGCCTGG CAAACCCACT ATGATCATCA GCACCACCGT TGGCAACACG 
GCCCTTATCC AGTGGCAGCC TCCTAAAGAC ATGGTGGGTG AGCTGATGGG CTACCGGCTG 
CGCTACAAAC GTCTGGAAGA GGAAAACTAT GAGATACGCG AATTCAGTCG CACCGACGAC 
CACCACACCG TCACTGACCT GCACAAGGGC GCCACCTACA GCTTCCACCT GAGCGCCAGA 
AACCGCGCTG GACTCGGAGA GGAGTACGTT AAAGACATCG GCACTCCTGA GGACGTGCCC 
AGCGGGTTTC CTCTTAACCT GCAGGTCGTA GGCTTGACTA CCTCTACCAC CCGACTGACC 
TGGGAGCCTC CAGCGTTGTC TGAACGTAAC GGACGGATCA TACATTACAT TGTGGTTTAC 
CGAGACATCA ACAGCAAACA AAACAGCACC AACCGAACCT CCGACACACA GATGACCATC 
CAAGGGCTCC GACCTGACAC CACCTATGAT ATCCGTGTGC AGGCGTTCAC TAGCAAGGGT 
GGAGGTCCCA TAAGTCCCAG TATCCAGAGC AGGACCATGT CTACGTCACC TGCATTTGCC 
ACAAGTTTTG GTGTCAAAGC TGTGATGAAA ACCTCTGTCC TTCTCACCTG GGAGGTGCCA 
GAAAATTATA AATCACAAGT GCCTTTTAAG ATCCTCTATA ACCAGCAGAG TGTGGAAGTC 
CAGGGGAATC TGAAGAGGAA GCTGATCACA CGCCTGCAGC CGGACACTGA TTACTCGTTT 
GTGCTGATGA GCCGGGGAAA CAGCGCCGGT GGCCTACAAC AGCAGGTCTC CATCCGCACT 
GCACCAGACC TGTTCAAAAC TAAACCTGTC CTGTACACGG CAGACCAGAC CAACAATGGC 
AAACTCACCA TCAACCTGCC CAAAGTGCCC ACCTCTGCTC CTGTCAGGTG TTATTACATT 
GTGGTCGTGC CCGTCTCTCA GACATCATCT CGTCAGTGGA TTAATCCCGA TGAGATGGAG 
CTGGATGAGC TCCTGGAGTC TAGTGAGGGA GCTCATCTCA GGAGACGTCG ACGGCACACA 
GACACCGTAA GACCGTACAT CGCCGCAAAG CTATCCACGC TCCCAGAAAC CTTCACTCTG 
GGCGATGAGC TAGACTACAG CGGCTTCATC AACCGGCCTC TTCCCAACAA CCAACACTTC 
CAGTGTTTTG TGATGGCTGA ACTCAAGGAC CAATACCCTG TTACTGCCAA TGAGAAGCAG 
CGGACCTTCT CGGCTAGCCC ATACTCAGAC CCCATCATAG TGCAGGGTGA AAACCAAATG 
CAGCGCAGTG TGGACCAACC GGAGATGCTT TGGGTCATGG GCCCTGTGCT CGCTGTGGTG 
CTCATCATCA TCATAGTCAT CGCCATCCTG CTCTTCAAAA GCAAACAGGA GAGGAAGAGG 
GCTTCCCCCC TACCAAAAGA CGATCATTCG GGTGGTGTGA AGGATTGTCT TCTGGCCAGC 
TCATCCGATC CTGTCGAAAT GAGGAGACTC AACTACCAGA CACCAGGAAT GAGGGAACAT 
CCTCCCATAT CCGTCTCCGA ACTGGCAGAC CACATCGAGC GCCTCAAGGC CAACGATGCC 
CTCCGGTTTT CCCAAGAGTA TGAGTCTATT GATCCAGGCC AGCAGTTCAC ATGGGAGAAC 
TCCAACTTGG AGGTCAACAA GCCAAAGAAC CGTTATGCCA ACGTTATTGC TTACGACCAC 
TCGAGAGTTG TTCTCACCCC TGTGGACGGT GTACCAGGGA GTGACTACAT TAATTCCAAC 
TACATTGATG GCTACCGTAA GCAGAATGCC TACATTGCCA CACAAGGTCC TCTGCCTGAG 
ACTCTGAGCG ACTTCTGGAG GATGATTTGG GAGCAACGAG CCAGTACCAT CGTCATGATG 
ACCAGACTGG AAGAGAAGTC CAGAGTCAAG TGTGACCAAT ACTGGCCCAT TCGTGGCACA 
GAGACTTATG GAATGATTCA AGTGACCATG CTTGATGCTG TTGAGCTGGC AACCTACAGT 
GTGCGCACCT TTGCCCTTTA CAAGAATGGC TCTAGTGAGA AGCGCGAGGT CAGGCAGTTT 
CAGTTCATGG CTTGGCCGGA CCACGGAGTG CCTGAATATC CCACCCCCAT CCTAGCCTTC 
CTGCGCAGGG TCAAAGCCTG CAACCCTCCT GATGCTGGAC CCATGGTAGT GCACTGCAGT 
GCTGGAGTTG GCCGTACAGG CTGCTTCATA GTCATCGATG CGATGTTGGA GCGCATGAAG 
CACGAGAAGA CCGTAGACAT TTATGGCCAC GTCACGTGCA TGAGATCCCA GAGGAACTAT 
ATGGTGCAGA CGGAGGACCA GTACATCTTT ATCCACGAGG CCCTTCTGGA GGCCGCCACC 
TGTGGAAACA CAGAGGTCCC TGCCCGAAAC CTCTACGCCC ACATCCAGAA GCTCACACAG 
CCATCGGCTG GAGAGACCGT CACAGCCATG GAGCTGGAGT TTAAGAGACT CGCCAACTCC 
AAAGCCCACA CCTCAAGATT CATCAGCGCC AACCTGCCCT GCAACAAGTT CAAGAACCGG 
CTGGTCAATA TCATGCCTTT CGAGTCCACA CGGGTGTGCC TGCAGCCAAT CCGAGGAGTG 
GAGGGGTCCG ACTACATCAA TGCCAGCTGC ATTGACGGAT ACAGACAGCA GAAGGCCTAT 
ATTGCCACTC AGGGTCCTCT AGCGGAGACC ACCGAGGACT TCTGGAGGAT GTTATGGGAG 
CACAATTCCA CTATTGTAGT GATGCTGACC AAACTCAGAG AGATGGGACG GGAGAAGTGT 
CATCAGTACT GGCCCGCCGA GCGCTCAGCC CGCTATCAGT ACTTCGTAGT GGATCCCATG 
GCCGAATACA ACATGCCCCA GTACATCCTG CGAGAGTTCA AAGTCACAGA TGCCAGGGAC 
GGTCAATCCA GAACCATTCG CCAGTTCCAG TTCACTGATT GGCCAGAGCA GGGAGTGCCA 
AAAACCGGTG AGGGGTTCAT CGATTTCATT GGGCAAGTTC ATAAAACCAA GGAGCAGTTT 
GGGCAGGATG GACCAATCAC TGTGCACTGC AGTGCTGGAG TGGGAAGAAC AGGTGTTTTC 
ATCACTCTCA GTATTGTCCT GGAGAGGATG AGATATGAGG GGGTGGTGGA TCTGTTCCAG 
ACGGTTAAAA CCCTGCGCAC ACAAAGGCCT GCCATGGTGC AGACAGAGGA CCAGTACCAG 
CTGTGCTACA GAGCCGCTCT GGAGTACCTG GGGAGCTTTG ATCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296738.1
CDS	422..6178
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVIREDPRGVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298463.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298463.3

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGGAGGC TGTGATCGCT GGACTTGCCT CTGAAACCTC TTACTCAGTG 
ACTGTGGCTG CGTACACAAC CAAAGGTGAT GGAGCTCGCA GCAAAGCCAA AGTGGTCACC 
ACCACCGGAG CAGTGCCTGG CAAACCCACT ATGATCATCA GCACCACCGT TGGCAACACG 
GCCCTTATCC AGTGGCAGCC TCCTAAAGAC ATGGTGGGTG AGCTGATGGG CTACCGGCTG 
CGCTACAAAC GTCTGGAAGA GGAAAACTAT GAGATACGCG AATTCAGTCG CACCGACGAC 
CACCACACCG TCACTGACCT GCACAAGGGC GCCACCTACA GCTTCCACCT GAGCGCCAGA 
AACCGCGCTG GACTCGGAGA GGAGTACGTT AAAGACATCG GCACTCCTGA GGACGTGCCC 
AGCGGGTTTC CTCTTAACCT GCAGGTCGTA GGCTTGACTA CCTCTACCAC CCGACTGACC 
TGGGAGCCTC CAGCGTTGTC TGAACGTAAC GGACGGATCA TACATTACAT TGTGGTTTAC 
CGAGACATCA ACAGCAAACA AAACAGCACC AACCGAACCT CCGACACACA GATGACCATC 
CAAGGGCTCC GACCTGACAC CACCTATGAT ATCCGTGTGC AGGCGTTCAC TAGCAAGGGT 
GGAGGTCCCA TAAGTCCCAG TATCCAGAGC AGGACCATGT CTACGTCACC TGCATTTGCC 
ACAAGTTTTG GTGTCAAAGC TGTGATGAAA ACCTCTGTCC TTCTCACCTG GGAGGTGCCA 
GAAAATTATA AATCACAAGT GCCTTTTAAG ATCCTCTATA ACCAGCAGAG TGTGGAAGTC 
CAGGGGAATC TGAAGAGGAA GCTGATCACA CGCCTGCAGC CGGACACTGA TTACTCGTTT 
GTGCTGATGA GCCGGGGAAA CAGCGCCGGT GGCCTACAAC AGCAGGTCTC CATCCGCACT 
GCACCAGACC TGTTCAAAAC TAAACCTGTC CTGTACACGG CAGACCAGAC CAACAATGGC 
AAACTCACCA TCAACCTGCC CAAAGTGCCC ACCTCTGCTC CTGTCAGGTG TTATTACATT 
GTGGTCGTGC CCGTCTCTCA GACATCATCT CGTCAGTGGA TTAATCCCGA TGAGATGGAG 
CTGGATGAGC TCCTGGAGTC TAGTGAGGGA GCTCATCTCA GGAGACGTCG ACGGCACACA 
GACACCGTAA GACCGTACAT CGCCGCAAAG CTATCCACGC TCCCAGAAAC CTTCACTCTG 
GGCGATGAGC TAGACTACAG CGGCTTCATC AACCGGCCTC TTCCCAACAA CCAACACTTC 
CAGTGTTTTG TGATGGCTGA ACTCAAGGAC CAATACCCTG TTACTGCCAA TGAGAAGCAG 
CGGACCTTCT CGGCTAGCCC ATACTCAGAC CCCATCATAG TGCAGGGTGA AAACCAAATG 
CAGCGCAGTG TGGACCAACC GGAGATGCTT TGGGTCATGG GCCCTGTGCT CGCTGTGGTG 
CTCATCATCA TCATAGTCAT CGCCATCCTG CTCTTCAAAA GCAAACAGGA GAGGAAGAGG 
GCTTCCCCCC TACCAAAAGA CGATCATTCG GGTGGTGTGA AGGATTGTCT TCTGGCCAGC 
TCATCCGATC CTGTCGAAAT GAGGAGACTC AACTACCAGA CACCAGGAAT GAGGGAACAT 
CCTCCCATAT CCGTCTCCGA ACTGGCAGAC CACATCGAGC GCCTCAAGGC CAACGATGCC 
CTCCGGTTTT CCCAAGAGTA TGAGTCTATT GATCCAGGCC AGCAGTTCAC ATGGGAGAAC 
TCCAACTTGG AGGTCAACAA GCCAAAGAAC CGTTATGCCA ACGTTATTGC TTACGACCAC 
TCGAGAGTTG TTCTCACCCC TGTGGACGGT GTACCAGGGA GTGACTACAT TAATTCCAAC 
TACATTGATG GCTACCGTAA GCAGAATGCC TACATTGCCA CACAAGGTCC TCTGCCTGAG 
ACTCTGAGCG ACTTCTGGAG GATGATTTGG GAGCAACGAG CCAGTACCAT CGTCATGATG 
ACCAGACTGG AAGAGAAGTC CAGAGTCAAG TGTGACCAAT ACTGGCCCAT TCGTGGCACA 
GAGACTTATG GAATGATTCA AGTGACCATG CTTGATGCTG TTGAGCTGGC AACCTACAGT 
GTGCGCACCT TTGCCCTTTA CAAGAATGGC TCTAGTGAGA AGCGCGAGGT CAGGCAGTTT 
CAGTTCATGG CTTGGCCGGA CCACGGAGTG CCTGAATATC CCACCCCCAT CCTAGCCTTC 
CTGCGCAGGG TCAAAGCCTG CAACCCTCCT GATGCTGGAC CCATGGTAGT GCACTGCAGT 
GCTGGAGTTG GCCGTACAGG CTGCTTCATA GTCATCGATG CGATGTTGGA GCGCATGAAG 
CACGAGAAGA CCGTAGACAT TTATGGCCAC GTCACGTGCA TGAGATCCCA GAGGAACTAT 
ATGGTGCAGA CGGAGGACCA GTACATCTTT ATCCACGAGG CCCTTCTGGA GGCCGCCACC 
TGTGGAAACA CAGAGGTCCC TGCCCGAAAC CTCTACGCCC ACATCCAGAA GCTCACACAG 
CCATCGGCTG GAGAGACCGT CACAGCCATG GAGCTGGAGT TTAAGAGACT CGCCAACTCC 
AAAGCCCACA CCTCAAGATT CATCAGCGCC AACCTGCCCT GCAACAAGTT CAAGAACCGG 
CTGGTCAATA TCATGCCTTT CGAGTCCACA CGGGTGTGCC TGCAGCCAAT CCGAGGAGTG 
GAGGGGTCCG ACTACATCAA TGCCAGCTGC ATTGACGGAT ACAGACAGCA GAAGGCCTAT 
ATTGCCACTC AGGGTCCTCT AGCGGAGACC ACCGAGGACT TCTGGAGGAT GTTATGGGAG 
CACAATTCCA CTATTGTAGT GATGCTGACC AAACTCAGAG AGATGGGACG GGAGAAGTGT 
CATCAGTACT GGCCCGCCGA GCGCTCAGCC CGCTATCAGT ACTTCGTAGT GGATCCCATG 
GCCGAATACA ACATGCCCCA GTACATCCTG CGAGAGTTCA AAGTCACAGA TGCCAGGGAC 
GGTCAATCCA GAACCATTCG CCAGTTCCAG TTCACTGATT GGCCAGAGCA GGGAGTGCCA 
AAAACCGGTG AGGGGTTCAT CGATTTCATT GGGCAAGTTC ATAAAACCAA GGAGCAGTTT 
GGGCAGGATG GACCAATCAC TGTGCACTGC AGTGCTGGAG TGGGAAGAAC AGGTGTTTTC 
ATCACTCTCA GTATTGTCCT GGAGAGGATG AGATATGAGG GGGTGGTGGA TCTGTTCCAG 
ACGGTTAAAA CCCTGCGCAC ACAAAGGCCT GCCATGGTGC AGACAGAGGA CCAGTACCAG 
CTGTGCTACA GAGCCGCTCT GGAGTACCTG GGGAGCTTTG ATCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298463.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296738.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X7, mRNA.

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CloneID	ODa47418
Clone ID Related Accession (Same CDS sequence)	XM_009298463.3 , XM_009298463.2
Accession Version	XM_009298463.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298463.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGGAGGC TGTGATCGCT GGACTTGCCT CTGAAACCTC TTACTCAGTG 
ACTGTGGCTG CGTACACAAC CAAAGGTGAT GGAGCTCGCA GCAAAGCCAA AGTGGTCACC 
ACCACCGGAG CAGTGCCTGG CAAACCCACT ATGATCATCA GCACCACCGT TGGCAACACG 
GCCCTTATCC AGTGGCAGCC TCCTAAAGAC ATGGTGGGTG AGCTGATGGG CTACCGGCTG 
CGCTACAAAC GTCTGGAAGA GGAAAACTAT GAGATACGCG AATTCAGTCG CACCGACGAC 
CACCACACCG TCACTGACCT GCACAAGGGC GCCACCTACA GCTTCCACCT GAGCGCCAGA 
AACCGCGCTG GACTCGGAGA GGAGTACGTT AAAGACATCG GCACTCCTGA GGACGTGCCC 
AGCGGGTTTC CTCTTAACCT GCAGGTCGTA GGCTTGACTA CCTCTACCAC CCGACTGACC 
TGGGAGCCTC CAGCGTTGTC TGAACGTAAC GGACGGATCA TACATTACAT TGTGGTTTAC 
CGAGACATCA ACAGCAAACA AAACAGCACC AACCGAACCT CCGACACACA GATGACCATC 
CAAGGGCTCC GACCTGACAC CACCTATGAT ATCCGTGTGC AGGCGTTCAC TAGCAAGGGT 
GGAGGTCCCA TAAGTCCCAG TATCCAGAGC AGGACCATGT CTACGTCACC TGCATTTGCC 
ACAAGTTTTG GTGTCAAAGC TGTGATGAAA ACCTCTGTCC TTCTCACCTG GGAGGTGCCA 
GAAAATTATA AATCACAAGT GCCTTTTAAG ATCCTCTATA ACCAGCAGAG TGTGGAAGTC 
CAGGGGAATC TGAAGAGGAA GCTGATCACA CGCCTGCAGC CGGACACTGA TTACTCGTTT 
GTGCTGATGA GCCGGGGAAA CAGCGCCGGT GGCCTACAAC AGCAGGTCTC CATCCGCACT 
GCACCAGACC TGTTCAAAAC TAAACCTGTC CTGTACACGG CAGACCAGAC CAACAATGGC 
AAACTCACCA TCAACCTGCC CAAAGTGCCC ACCTCTGCTC CTGTCAGGTG TTATTACATT 
GTGGTCGTGC CCGTCTCTCA GACATCATCT CGTCAGTGGA TTAATCCCGA TGAGATGGAG 
CTGGATGAGC TCCTGGAGTC TAGTGAGGGA GCTCATCTCA GGAGACGTCG ACGGCACACA 
GACACCGTAA GACCGTACAT CGCCGCAAAG CTATCCACGC TCCCAGAAAC CTTCACTCTG 
GGCGATGAGC TAGACTACAG CGGCTTCATC AACCGGCCTC TTCCCAACAA CCAACACTTC 
CAGTGTTTTG TGATGGCTGA ACTCAAGGAC CAATACCCTG TTACTGCCAA TGAGAAGCAG 
CGGACCTTCT CGGCTAGCCC ATACTCAGAC CCCATCATAG TGCAGGGTGA AAACCAAATG 
CAGCGCAGTG TGGACCAACC GGAGATGCTT TGGGTCATGG GCCCTGTGCT CGCTGTGGTG 
CTCATCATCA TCATAGTCAT CGCCATCCTG CTCTTCAAAA GCAAACAGGA GAGGAAGAGG 
GCTTCCCCCC TACCAAAAGA CGATCATTCG GGTGGTGTGA AGGATTGTCT TCTGGCCAGC 
TCATCCGATC CTGTCGAAAT GAGGAGACTC AACTACCAGA CACCAGGAAT GAGGGAACAT 
CCTCCCATAT CCGTCTCCGA ACTGGCAGAC CACATCGAGC GCCTCAAGGC CAACGATGCC 
CTCCGGTTTT CCCAAGAGTA TGAGTCTATT GATCCAGGCC AGCAGTTCAC ATGGGAGAAC 
TCCAACTTGG AGGTCAACAA GCCAAAGAAC CGTTATGCCA ACGTTATTGC TTACGACCAC 
TCGAGAGTTG TTCTCACCCC TGTGGACGGT GTACCAGGGA GTGACTACAT TAATTCCAAC 
TACATTGATG GCTACCGTAA GCAGAATGCC TACATTGCCA CACAAGGTCC TCTGCCTGAG 
ACTCTGAGCG ACTTCTGGAG GATGATTTGG GAGCAACGAG CCAGTACCAT CGTCATGATG 
ACCAGACTGG AAGAGAAGTC CAGAGTCAAG TGTGACCAAT ACTGGCCCAT TCGTGGCACA 
GAGACTTATG GAATGATTCA AGTGACCATG CTTGATGCTG TTGAGCTGGC AACCTACAGT 
GTGCGCACCT TTGCCCTTTA CAAGAATGGC TCTAGTGAGA AGCGCGAGGT CAGGCAGTTT 
CAGTTCATGG CTTGGCCGGA CCACGGAGTG CCTGAATATC CCACCCCCAT CCTAGCCTTC 
CTGCGCAGGG TCAAAGCCTG CAACCCTCCT GATGCTGGAC CCATGGTAGT GCACTGCAGT 
GCTGGAGTTG GCCGTACAGG CTGCTTCATA GTCATCGATG CGATGTTGGA GCGCATGAAG 
CACGAGAAGA CCGTAGACAT TTATGGCCAC GTCACGTGCA TGAGATCCCA GAGGAACTAT 
ATGGTGCAGA CGGAGGACCA GTACATCTTT ATCCACGAGG CCCTTCTGGA GGCCGCCACC 
TGTGGAAACA CAGAGGTCCC TGCCCGAAAC CTCTACGCCC ACATCCAGAA GCTCACACAG 
CCATCGGCTG GAGAGACCGT CACAGCCATG GAGCTGGAGT TTAAGAGACT CGCCAACTCC 
AAAGCCCACA CCTCAAGATT CATCAGCGCC AACCTGCCCT GCAACAAGTT CAAGAACCGG 
CTGGTCAATA TCATGCCTTT CGAGTCCACA CGGGTGTGCC TGCAGCCAAT CCGAGGAGTG 
GAGGGGTCCG ACTACATCAA TGCCAGCTGC ATTGACGGAT ACAGACAGCA GAAGGCCTAT 
ATTGCCACTC AGGGTCCTCT AGCGGAGACC ACCGAGGACT TCTGGAGGAT GTTATGGGAG 
CACAATTCCA CTATTGTAGT GATGCTGACC AAACTCAGAG AGATGGGACG GGAGAAGTGT 
CATCAGTACT GGCCCGCCGA GCGCTCAGCC CGCTATCAGT ACTTCGTAGT GGATCCCATG 
GCCGAATACA ACATGCCCCA GTACATCCTG CGAGAGTTCA AAGTCACAGA TGCCAGGGAC 
GGTCAATCCA GAACCATTCG CCAGTTCCAG TTCACTGATT GGCCAGAGCA GGGAGTGCCA 
AAAACCGGTG AGGGGTTCAT CGATTTCATT GGGCAAGTTC ATAAAACCAA GGAGCAGTTT 
GGGCAGGATG GACCAATCAC TGTGCACTGC AGTGCTGGAG TGGGAAGAAC AGGTGTTTTC 
ATCACTCTCA GTATTGTCCT GGAGAGGATG AGATATGAGG GGGTGGTGGA TCTGTTCCAG 
ACGGTTAAAA CCCTGCGCAC ACAAAGGCCT GCCATGGTGC AGACAGAGGA CCAGTACCAG 
CTGTGCTACA GAGCCGCTCT GGAGTACCTG GGGAGCTTTG ATCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296738.1
CDS	426..6182
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVIREDPRGVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298463.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298463.2

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGCCCCC 
TCTGCCCCCC CTCAAGACGT GCACCTGCTC AGTCTGAGCT CCACCAGCAT CAAGGTGAGT 
TGGGTGGCAC CACCCGCCGC TAGCCGCCAC GGCGCAATAG TGCGCTACAC AGTCAGCTAC 
CAGGCAGTGA ATGGAGAAGA CACAGAGCGC CATGAGGTGC CGGACATTGG AGCGGATGCC 
TCCAGCTGTG TGTTGGAAGG TCTAGAGAAG TGGACGGAGT ATCAGGTGTG GGTGAGAGCG 
CACACTGATG TGGGCCCCGG CCCCGAGAGC ACGGCTGTGC GTATCCGAAC CAATGAAGAC 
GTGCCAGGAG CACCGCCTCG GAAATTGGAA GTGGAGGCTT TGAACTCCAC CGCTATCCGT 
GTGACATGGA AACCTCCTCT TTCTGGAAAG CAGCATGGCC AGATCCGGGG ATATCAGGTC 
ATATACTCCC GGTTGGAGAA CGGGGAACCA CGGGGACATC CGAATATAAT GGATGTCGCT 
CTGCCAGAAG CACAGGAGGC TGTGATCGCT GGACTTGCCT CTGAAACCTC TTACTCAGTG 
ACTGTGGCTG CGTACACAAC CAAAGGTGAT GGAGCTCGCA GCAAAGCCAA AGTGGTCACC 
ACCACCGGAG CAGTGCCTGG CAAACCCACT ATGATCATCA GCACCACCGT TGGCAACACG 
GCCCTTATCC AGTGGCAGCC TCCTAAAGAC ATGGTGGGTG AGCTGATGGG CTACCGGCTG 
CGCTACAAAC GTCTGGAAGA GGAAAACTAT GAGATACGCG AATTCAGTCG CACCGACGAC 
CACCACACCG TCACTGACCT GCACAAGGGC GCCACCTACA GCTTCCACCT GAGCGCCAGA 
AACCGCGCTG GACTCGGAGA GGAGTACGTT AAAGACATCG GCACTCCTGA GGACGTGCCC 
AGCGGGTTTC CTCTTAACCT GCAGGTCGTA GGCTTGACTA CCTCTACCAC CCGACTGACC 
TGGGAGCCTC CAGCGTTGTC TGAACGTAAC GGACGGATCA TACATTACAT TGTGGTTTAC 
CGAGACATCA ACAGCAAACA AAACAGCACC AACCGAACCT CCGACACACA GATGACCATC 
CAAGGGCTCC GACCTGACAC CACCTATGAT ATCCGTGTGC AGGCGTTCAC TAGCAAGGGT 
GGAGGTCCCA TAAGTCCCAG TATCCAGAGC AGGACCATGT CTACGTCACC TGCATTTGCC 
ACAAGTTTTG GTGTCAAAGC TGTGATGAAA ACCTCTGTCC TTCTCACCTG GGAGGTGCCA 
GAAAATTATA AATCACAAGT GCCTTTTAAG ATCCTCTATA ACCAGCAGAG TGTGGAAGTC 
CAGGGGAATC TGAAGAGGAA GCTGATCACA CGCCTGCAGC CGGACACTGA TTACTCGTTT 
GTGCTGATGA GCCGGGGAAA CAGCGCCGGT GGCCTACAAC AGCAGGTCTC CATCCGCACT 
GCACCAGACC TGTTCAAAAC TAAACCTGTC CTGTACACGG CAGACCAGAC CAACAATGGC 
AAACTCACCA TCAACCTGCC CAAAGTGCCC ACCTCTGCTC CTGTCAGGTG TTATTACATT 
GTGGTCGTGC CCGTCTCTCA GACATCATCT CGTCAGTGGA TTAATCCCGA TGAGATGGAG 
CTGGATGAGC TCCTGGAGTC TAGTGAGGGA GCTCATCTCA GGAGACGTCG ACGGCACACA 
GACACCGTAA GACCGTACAT CGCCGCAAAG CTATCCACGC TCCCAGAAAC CTTCACTCTG 
GGCGATGAGC TAGACTACAG CGGCTTCATC AACCGGCCTC TTCCCAACAA CCAACACTTC 
CAGTGTTTTG TGATGGCTGA ACTCAAGGAC CAATACCCTG TTACTGCCAA TGAGAAGCAG 
CGGACCTTCT CGGCTAGCCC ATACTCAGAC CCCATCATAG TGCAGGGTGA AAACCAAATG 
CAGCGCAGTG TGGACCAACC GGAGATGCTT TGGGTCATGG GCCCTGTGCT CGCTGTGGTG 
CTCATCATCA TCATAGTCAT CGCCATCCTG CTCTTCAAAA GCAAACAGGA GAGGAAGAGG 
GCTTCCCCCC TACCAAAAGA CGATCATTCG GGTGGTGTGA AGGATTGTCT TCTGGCCAGC 
TCATCCGATC CTGTCGAAAT GAGGAGACTC AACTACCAGA CACCAGGAAT GAGGGAACAT 
CCTCCCATAT CCGTCTCCGA ACTGGCAGAC CACATCGAGC GCCTCAAGGC CAACGATGCC 
CTCCGGTTTT CCCAAGAGTA TGAGTCTATT GATCCAGGCC AGCAGTTCAC ATGGGAGAAC 
TCCAACTTGG AGGTCAACAA GCCAAAGAAC CGTTATGCCA ACGTTATTGC TTACGACCAC 
TCGAGAGTTG TTCTCACCCC TGTGGACGGT GTACCAGGGA GTGACTACAT TAATTCCAAC 
TACATTGATG GCTACCGTAA GCAGAATGCC TACATTGCCA CACAAGGTCC TCTGCCTGAG 
ACTCTGAGCG ACTTCTGGAG GATGATTTGG GAGCAACGAG CCAGTACCAT CGTCATGATG 
ACCAGACTGG AAGAGAAGTC CAGAGTCAAG TGTGACCAAT ACTGGCCCAT TCGTGGCACA 
GAGACTTATG GAATGATTCA AGTGACCATG CTTGATGCTG TTGAGCTGGC AACCTACAGT 
GTGCGCACCT TTGCCCTTTA CAAGAATGGC TCTAGTGAGA AGCGCGAGGT CAGGCAGTTT 
CAGTTCATGG CTTGGCCGGA CCACGGAGTG CCTGAATATC CCACCCCCAT CCTAGCCTTC 
CTGCGCAGGG TCAAAGCCTG CAACCCTCCT GATGCTGGAC CCATGGTAGT GCACTGCAGT 
GCTGGAGTTG GCCGTACAGG CTGCTTCATA GTCATCGATG CGATGTTGGA GCGCATGAAG 
CACGAGAAGA CCGTAGACAT TTATGGCCAC GTCACGTGCA TGAGATCCCA GAGGAACTAT 
ATGGTGCAGA CGGAGGACCA GTACATCTTT ATCCACGAGG CCCTTCTGGA GGCCGCCACC 
TGTGGAAACA CAGAGGTCCC TGCCCGAAAC CTCTACGCCC ACATCCAGAA GCTCACACAG 
CCATCGGCTG GAGAGACCGT CACAGCCATG GAGCTGGAGT TTAAGAGACT CGCCAACTCC 
AAAGCCCACA CCTCAAGATT CATCAGCGCC AACCTGCCCT GCAACAAGTT CAAGAACCGG 
CTGGTCAATA TCATGCCTTT CGAGTCCACA CGGGTGTGCC TGCAGCCAAT CCGAGGAGTG 
GAGGGGTCCG ACTACATCAA TGCCAGCTGC ATTGACGGAT ACAGACAGCA GAAGGCCTAT 
ATTGCCACTC AGGGTCCTCT AGCGGAGACC ACCGAGGACT TCTGGAGGAT GTTATGGGAG 
CACAATTCCA CTATTGTAGT GATGCTGACC AAACTCAGAG AGATGGGACG GGAGAAGTGT 
CATCAGTACT GGCCCGCCGA GCGCTCAGCC CGCTATCAGT ACTTCGTAGT GGATCCCATG 
GCCGAATACA ACATGCCCCA GTACATCCTG CGAGAGTTCA AAGTCACAGA TGCCAGGGAC 
GGTCAATCCA GAACCATTCG CCAGTTCCAG TTCACTGATT GGCCAGAGCA GGGAGTGCCA 
AAAACCGGTG AGGGGTTCAT CGATTTCATT GGGCAAGTTC ATAAAACCAA GGAGCAGTTT 
GGGCAGGATG GACCAATCAC TGTGCACTGC AGTGCTGGAG TGGGAAGAAC AGGTGTTTTC 
ATCACTCTCA GTATTGTCCT GGAGAGGATG AGATATGAGG GGGTGGTGGA TCTGTTCCAG 
ACGGTTAAAA CCCTGCGCAC ACAAAGGCCT GCCATGGTGC AGACAGAGGA CCAGTACCAG 
CTGTGCTACA GAGCCGCTCT GGAGTACCTG GGGAGCTTTG ATCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298464.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296739.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X10, mRNA.

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CloneID	ODa47419
Clone ID Related Accession (Same CDS sequence)	XM_009298464.3 , XM_009298464.2
Accession Version	XM_009298464.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298464.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT CAAGACGTGC ACCTGCTCAG TCTGAGCTCC 
ACCAGCATCA AGGTGAGTTG GGTGGCACCA CCCGCCGCTA GCCGCCACGG CGCAATAGTG 
CGCTACACAG TCAGCTACCA GGCAGTGAAT GGAGAAGACA CAGAGCGCCA TGAGGTGCCG 
GACATTGGAG CGGATGCCTC CAGCTGTGTG TTGGAAGGTC TAGAGAAGTG GACGGAGTAT 
CAGGTGTGGG TGAGAGCGCA CACTGATGTG GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT 
ATCCGAACCA ATGAAGACGT GCCAGGAGCA CCGCCTCGGA AATTGGAAGT GGAGGCTTTG 
AACTCCACCG CTATCCGTGT GACATGGAAA CCTCCTCTTT CTGGAAAGCA GCATGGCCAG 
ATCCGGGGAT ATCAGGTCAT ATACTCCCGG TTGGAGAACG GGGAACCACG GGGACATCCG 
AATATAATGG ATGTCGCTCT GCCAGAAGCA CAGTGGAAAA TTGAAGAGTC TACGGAATAT 
GAGGCTGTGA TCGCTGGACT TGCCTCTGAA ACCTCTTACT CAGTGACTGT GGCTGCGTAC 
ACAACCAAAG GTGATGGAGC TCGCAGCAAA GCCAAAGTGG TCACCACCAC CGGAGCAGTG 
CCTGGCAAAC CCACTATGAT CATCAGCACC ACCGTTGGCA ACACGGCCCT TATCCAGTGG 
CAGCCTCCTA AAGACATGGT GGGTGAGCTG ATGGGCTACC GGCTGCGCTA CAAACGTCTG 
GAAGAGGAAA ACTATGAGAT ACGCGAATTC AGTCGCACCG ACGACCACCA CACCGTCACT 
GACCTGCACA AGGGCGCCAC CTACAGCTTC CACCTGAGCG CCAGAAACCG CGCTGGACTC 
GGAGAGGAGT ACGTTAAAGA CATCGGCACT CCTGAGGACG TGCCCAGCGG GTTTCCTCTT 
AACCTGCAGG TCGTAGGCTT GACTACCTCT ACCACCCGAC TGACCTGGGA GCCTCCAGCG 
TTGTCTGAAC GTAACGGACG GATCATACAT TACATTGTGG TTTACCGAGA CATCAACAGC 
AAACAAAACA GCACCAACCG AACCTCCGAC ACACAGATGA CCATCCAAGG GCTCCGACCT 
GACACCACCT ATGATATCCG TGTGCAGGCG TTCACTAGCA AGGGTGGAGG TCCCATAAGT 
CCCAGTATCC AGAGCAGGAC CATGTCTACG TCACCTGCAT TTGCCACAAG TTTTGGTGTC 
AAAGCTGTGA TGAAAACCTC TGTCCTTCTC ACCTGGGAGG TGCCAGAAAA TTATAAATCA 
CAAGTGCCTT TTAAGATCCT CTATAACCAG CAGAGTGTGG AAGTCCAGGG GAATCTGAAG 
AGGAAGCTGA TCACACGCCT GCAGCCGGAC ACTGATTACT CGTTTGTGCT GATGAGCCGG 
GGAAACAGCG CCGGTGGCCT ACAACAGCAG GTCTCCATCC GCACTGCACC AGACCTGTTC 
AAAACTAAAC CTGTCCTGTA CACGGCAGAC CAGACCAACA ATGGCAAACT CACCATCAAC 
CTGCCCAAAG TGCCCACCTC TGCTCCTGTC AGGTGTTATT ACATTGTGGT CGTGCCCGTC 
TCTCAGACAT CATCTCGTCA GTGGATTAAT CCCGATGAGA TGGAGCTGGA TGAGCTCCTG 
GAGTCTAGTG AGGGAGCTCA TCTCAGGAGA CGTCGACGGC ACACAGACAC CGTAAGACCG 
TACATCGCCG CAAAGCTATC CACGCTCCCA GAAACCTTCA CTCTGGGCGA TGAGCTAGAC 
TACAGCGGCT TCATCAACCG GCCTCTTCCC AACAACCAAC ACTTCCAGTG TTTTGTGATG 
GCTGAACTCA AGGACCAATA CCCTGTTACT GCCAATGAGA AGCAGCGGAC CTTCTCGGCT 
AGCCCATACT CAGACCCCAT CATAGTGCAG GGTGAAAACC AAATGCAGCG CAGTGTGGAC 
CAACCGGAGA TGCTTTGGGT CATGGGCCCT GTGCTCGCTG TGGTGCTCAT CATCATCATA 
GTCATCGCCA TCCTGCTCTT CAAAAGCAAA CAGGAGAGGA AGAGGGCTTC CCCCCTACCA 
AAAGACGATC ATTCGGGTGG TGTGAAGGAT TGTCTTCTGG CCAGCTCATC CGATCCTGTC 
GAAATGAGGA GACTCAACTA CCAGACACCA GGAATGAGGG AACATCCTCC CATATCCGTC 
TCCGAACTGG CAGACCACAT CGAGCGCCTC AAGGCCAACG ATGCCCTCCG GTTTTCCCAA 
GAGTATGAGT CTATTGATCC AGGCCAGCAG TTCACATGGG AGAACTCCAA CTTGGAGGTC 
AACAAGCCAA AGAACCGTTA TGCCAACGTT ATTGCTTACG ACCACTCGAG AGTTGTTCTC 
ACCCCTGTGG ACGGTGTACC AGGGAGTGAC TACATTAATT CCAACTACAT TGATGGCTAC 
CGTAAGCAGA ATGCCTACAT TGCCACACAA GGTCCTCTGC CTGAGACTCT GAGCGACTTC 
TGGAGGATGA TTTGGGAGCA ACGAGCCAGT ACCATCGTCA TGATGACCAG ACTGGAAGAG 
AAGTCCAGAG TCAAGTGTGA CCAATACTGG CCCATTCGTG GCACAGAGAC TTATGGAATG 
ATTCAAGTGA CCATGCTTGA TGCTGTTGAG CTGGCAACCT ACAGTGTGCG CACCTTTGCC 
CTTTACAAGA ATGGCTCTAG TGAGAAGCGC GAGGTCAGGC AGTTTCAGTT CATGGCTTGG 
CCGGACCACG GAGTGCCTGA ATATCCCACC CCCATCCTAG CCTTCCTGCG CAGGGTCAAA 
GCCTGCAACC CTCCTGATGC TGGACCCATG GTAGTGCACT GCAGTGCTGG AGTTGGCCGT 
ACAGGCTGCT TCATAGTCAT CGATGCGATG TTGGAGCGCA TGAAGCACGA GAAGACCGTA 
GACATTTATG GCCACGTCAC GTGCATGAGA TCCCAGAGGA ACTATATGGT GCAGACGGAG 
GACCAGTACA TCTTTATCCA CGAGGCCCTT CTGGAGGCCG CCACCTGTGG AAACACAGAG 
GTCCCTGCCC GAAACCTCTA CGCCCACATC CAGAAGCTCA CACAGCCATC GGCTGGAGAG 
ACCGTCACAG CCATGGAGCT GGAGTTTAAG AGACTCGCCA ACTCCAAAGC CCACACCTCA 
AGATTCATCA GCGCCAACCT GCCCTGCAAC AAGTTCAAGA ACCGGCTGGT CAATATCATG 
CCTTTCGAGT CCACACGGGT GTGCCTGCAG CCAATCCGAG GAGTGGAGGG GTCCGACTAC 
ATCAATGCCA GCTGCATTGA CGGATACAGA CAGCAGAAGG CCTATATTGC CACTCAGGGT 
CCTCTAGCGG AGACCACCGA GGACTTCTGG AGGATGTTAT GGGAGCACAA TTCCACTATT 
GTAGTGATGC TGACCAAACT CAGAGAGATG GGACGGGAGA AGTGTCATCA GTACTGGCCC 
GCCGAGCGCT CAGCCCGCTA TCAGTACTTC GTAGTGGATC CCATGGCCGA ATACAACATG 
CCCCAGTACA TCCTGCGAGA GTTCAAAGTC ACAGATGCCA GGGACGGTCA ATCCAGAACC 
ATTCGCCAGT TCCAGTTCAC TGATTGGCCA GAGCAGGGAG TGCCAAAAAC CGGTGAGGGG 
TTCATCGATT TCATTGGGCA AGTTCATAAA ACCAAGGAGC AGTTTGGGCA GGATGGACCA 
ATCACTGTGC ACTGCAGTGC TGGAGTGGGA AGAACAGGTG TTTTCATCAC TCTCAGTATT 
GTCCTGGAGA GGATGAGATA TGAGGGGGTG GTGGATCTGT TCCAGACGGT TAAAACCCTG 
CGCACACAAA GGCCTGCCAT GGTGCAGACA GAGGACCAGT ACCAGCTGTG CTACAGAGCC 
GCTCTGGAGT ACCTGGGGAG CTTTGATCAC TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296739.1
CDS	422..6163
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298464.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298464.3

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT CAAGACGTGC ACCTGCTCAG TCTGAGCTCC 
ACCAGCATCA AGGTGAGTTG GGTGGCACCA CCCGCCGCTA GCCGCCACGG CGCAATAGTG 
CGCTACACAG TCAGCTACCA GGCAGTGAAT GGAGAAGACA CAGAGCGCCA TGAGGTGCCG 
GACATTGGAG CGGATGCCTC CAGCTGTGTG TTGGAAGGTC TAGAGAAGTG GACGGAGTAT 
CAGGTGTGGG TGAGAGCGCA CACTGATGTG GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT 
ATCCGAACCA ATGAAGACGT GCCAGGAGCA CCGCCTCGGA AATTGGAAGT GGAGGCTTTG 
AACTCCACCG CTATCCGTGT GACATGGAAA CCTCCTCTTT CTGGAAAGCA GCATGGCCAG 
ATCCGGGGAT ATCAGGTCAT ATACTCCCGG TTGGAGAACG GGGAACCACG GGGACATCCG 
AATATAATGG ATGTCGCTCT GCCAGAAGCA CAGTGGAAAA TTGAAGAGTC TACGGAATAT 
GAGGCTGTGA TCGCTGGACT TGCCTCTGAA ACCTCTTACT CAGTGACTGT GGCTGCGTAC 
ACAACCAAAG GTGATGGAGC TCGCAGCAAA GCCAAAGTGG TCACCACCAC CGGAGCAGTG 
CCTGGCAAAC CCACTATGAT CATCAGCACC ACCGTTGGCA ACACGGCCCT TATCCAGTGG 
CAGCCTCCTA AAGACATGGT GGGTGAGCTG ATGGGCTACC GGCTGCGCTA CAAACGTCTG 
GAAGAGGAAA ACTATGAGAT ACGCGAATTC AGTCGCACCG ACGACCACCA CACCGTCACT 
GACCTGCACA AGGGCGCCAC CTACAGCTTC CACCTGAGCG CCAGAAACCG CGCTGGACTC 
GGAGAGGAGT ACGTTAAAGA CATCGGCACT CCTGAGGACG TGCCCAGCGG GTTTCCTCTT 
AACCTGCAGG TCGTAGGCTT GACTACCTCT ACCACCCGAC TGACCTGGGA GCCTCCAGCG 
TTGTCTGAAC GTAACGGACG GATCATACAT TACATTGTGG TTTACCGAGA CATCAACAGC 
AAACAAAACA GCACCAACCG AACCTCCGAC ACACAGATGA CCATCCAAGG GCTCCGACCT 
GACACCACCT ATGATATCCG TGTGCAGGCG TTCACTAGCA AGGGTGGAGG TCCCATAAGT 
CCCAGTATCC AGAGCAGGAC CATGTCTACG TCACCTGCAT TTGCCACAAG TTTTGGTGTC 
AAAGCTGTGA TGAAAACCTC TGTCCTTCTC ACCTGGGAGG TGCCAGAAAA TTATAAATCA 
CAAGTGCCTT TTAAGATCCT CTATAACCAG CAGAGTGTGG AAGTCCAGGG GAATCTGAAG 
AGGAAGCTGA TCACACGCCT GCAGCCGGAC ACTGATTACT CGTTTGTGCT GATGAGCCGG 
GGAAACAGCG CCGGTGGCCT ACAACAGCAG GTCTCCATCC GCACTGCACC AGACCTGTTC 
AAAACTAAAC CTGTCCTGTA CACGGCAGAC CAGACCAACA ATGGCAAACT CACCATCAAC 
CTGCCCAAAG TGCCCACCTC TGCTCCTGTC AGGTGTTATT ACATTGTGGT CGTGCCCGTC 
TCTCAGACAT CATCTCGTCA GTGGATTAAT CCCGATGAGA TGGAGCTGGA TGAGCTCCTG 
GAGTCTAGTG AGGGAGCTCA TCTCAGGAGA CGTCGACGGC ACACAGACAC CGTAAGACCG 
TACATCGCCG CAAAGCTATC CACGCTCCCA GAAACCTTCA CTCTGGGCGA TGAGCTAGAC 
TACAGCGGCT TCATCAACCG GCCTCTTCCC AACAACCAAC ACTTCCAGTG TTTTGTGATG 
GCTGAACTCA AGGACCAATA CCCTGTTACT GCCAATGAGA AGCAGCGGAC CTTCTCGGCT 
AGCCCATACT CAGACCCCAT CATAGTGCAG GGTGAAAACC AAATGCAGCG CAGTGTGGAC 
CAACCGGAGA TGCTTTGGGT CATGGGCCCT GTGCTCGCTG TGGTGCTCAT CATCATCATA 
GTCATCGCCA TCCTGCTCTT CAAAAGCAAA CAGGAGAGGA AGAGGGCTTC CCCCCTACCA 
AAAGACGATC ATTCGGGTGG TGTGAAGGAT TGTCTTCTGG CCAGCTCATC CGATCCTGTC 
GAAATGAGGA GACTCAACTA CCAGACACCA GGAATGAGGG AACATCCTCC CATATCCGTC 
TCCGAACTGG CAGACCACAT CGAGCGCCTC AAGGCCAACG ATGCCCTCCG GTTTTCCCAA 
GAGTATGAGT CTATTGATCC AGGCCAGCAG TTCACATGGG AGAACTCCAA CTTGGAGGTC 
AACAAGCCAA AGAACCGTTA TGCCAACGTT ATTGCTTACG ACCACTCGAG AGTTGTTCTC 
ACCCCTGTGG ACGGTGTACC AGGGAGTGAC TACATTAATT CCAACTACAT TGATGGCTAC 
CGTAAGCAGA ATGCCTACAT TGCCACACAA GGTCCTCTGC CTGAGACTCT GAGCGACTTC 
TGGAGGATGA TTTGGGAGCA ACGAGCCAGT ACCATCGTCA TGATGACCAG ACTGGAAGAG 
AAGTCCAGAG TCAAGTGTGA CCAATACTGG CCCATTCGTG GCACAGAGAC TTATGGAATG 
ATTCAAGTGA CCATGCTTGA TGCTGTTGAG CTGGCAACCT ACAGTGTGCG CACCTTTGCC 
CTTTACAAGA ATGGCTCTAG TGAGAAGCGC GAGGTCAGGC AGTTTCAGTT CATGGCTTGG 
CCGGACCACG GAGTGCCTGA ATATCCCACC CCCATCCTAG CCTTCCTGCG CAGGGTCAAA 
GCCTGCAACC CTCCTGATGC TGGACCCATG GTAGTGCACT GCAGTGCTGG AGTTGGCCGT 
ACAGGCTGCT TCATAGTCAT CGATGCGATG TTGGAGCGCA TGAAGCACGA GAAGACCGTA 
GACATTTATG GCCACGTCAC GTGCATGAGA TCCCAGAGGA ACTATATGGT GCAGACGGAG 
GACCAGTACA TCTTTATCCA CGAGGCCCTT CTGGAGGCCG CCACCTGTGG AAACACAGAG 
GTCCCTGCCC GAAACCTCTA CGCCCACATC CAGAAGCTCA CACAGCCATC GGCTGGAGAG 
ACCGTCACAG CCATGGAGCT GGAGTTTAAG AGACTCGCCA ACTCCAAAGC CCACACCTCA 
AGATTCATCA GCGCCAACCT GCCCTGCAAC AAGTTCAAGA ACCGGCTGGT CAATATCATG 
CCTTTCGAGT CCACACGGGT GTGCCTGCAG CCAATCCGAG GAGTGGAGGG GTCCGACTAC 
ATCAATGCCA GCTGCATTGA CGGATACAGA CAGCAGAAGG CCTATATTGC CACTCAGGGT 
CCTCTAGCGG AGACCACCGA GGACTTCTGG AGGATGTTAT GGGAGCACAA TTCCACTATT 
GTAGTGATGC TGACCAAACT CAGAGAGATG GGACGGGAGA AGTGTCATCA GTACTGGCCC 
GCCGAGCGCT CAGCCCGCTA TCAGTACTTC GTAGTGGATC CCATGGCCGA ATACAACATG 
CCCCAGTACA TCCTGCGAGA GTTCAAAGTC ACAGATGCCA GGGACGGTCA ATCCAGAACC 
ATTCGCCAGT TCCAGTTCAC TGATTGGCCA GAGCAGGGAG TGCCAAAAAC CGGTGAGGGG 
TTCATCGATT TCATTGGGCA AGTTCATAAA ACCAAGGAGC AGTTTGGGCA GGATGGACCA 
ATCACTGTGC ACTGCAGTGC TGGAGTGGGA AGAACAGGTG TTTTCATCAC TCTCAGTATT 
GTCCTGGAGA GGATGAGATA TGAGGGGGTG GTGGATCTGT TCCAGACGGT TAAAACCCTG 
CGCACACAAA GGCCTGCCAT GGTGCAGACA GAGGACCAGT ACCAGCTGTG CTACAGAGCC 
GCTCTGGAGT ACCTGGGGAG CTTTGATCAC TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298464.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296739.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X8, mRNA.

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CloneID	ODa47419
Clone ID Related Accession (Same CDS sequence)	XM_009298464.3 , XM_009298464.2
Accession Version	XM_009298464.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298464.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT CAAGACGTGC ACCTGCTCAG TCTGAGCTCC 
ACCAGCATCA AGGTGAGTTG GGTGGCACCA CCCGCCGCTA GCCGCCACGG CGCAATAGTG 
CGCTACACAG TCAGCTACCA GGCAGTGAAT GGAGAAGACA CAGAGCGCCA TGAGGTGCCG 
GACATTGGAG CGGATGCCTC CAGCTGTGTG TTGGAAGGTC TAGAGAAGTG GACGGAGTAT 
CAGGTGTGGG TGAGAGCGCA CACTGATGTG GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT 
ATCCGAACCA ATGAAGACGT GCCAGGAGCA CCGCCTCGGA AATTGGAAGT GGAGGCTTTG 
AACTCCACCG CTATCCGTGT GACATGGAAA CCTCCTCTTT CTGGAAAGCA GCATGGCCAG 
ATCCGGGGAT ATCAGGTCAT ATACTCCCGG TTGGAGAACG GGGAACCACG GGGACATCCG 
AATATAATGG ATGTCGCTCT GCCAGAAGCA CAGTGGAAAA TTGAAGAGTC TACGGAATAT 
GAGGCTGTGA TCGCTGGACT TGCCTCTGAA ACCTCTTACT CAGTGACTGT GGCTGCGTAC 
ACAACCAAAG GTGATGGAGC TCGCAGCAAA GCCAAAGTGG TCACCACCAC CGGAGCAGTG 
CCTGGCAAAC CCACTATGAT CATCAGCACC ACCGTTGGCA ACACGGCCCT TATCCAGTGG 
CAGCCTCCTA AAGACATGGT GGGTGAGCTG ATGGGCTACC GGCTGCGCTA CAAACGTCTG 
GAAGAGGAAA ACTATGAGAT ACGCGAATTC AGTCGCACCG ACGACCACCA CACCGTCACT 
GACCTGCACA AGGGCGCCAC CTACAGCTTC CACCTGAGCG CCAGAAACCG CGCTGGACTC 
GGAGAGGAGT ACGTTAAAGA CATCGGCACT CCTGAGGACG TGCCCAGCGG GTTTCCTCTT 
AACCTGCAGG TCGTAGGCTT GACTACCTCT ACCACCCGAC TGACCTGGGA GCCTCCAGCG 
TTGTCTGAAC GTAACGGACG GATCATACAT TACATTGTGG TTTACCGAGA CATCAACAGC 
AAACAAAACA GCACCAACCG AACCTCCGAC ACACAGATGA CCATCCAAGG GCTCCGACCT 
GACACCACCT ATGATATCCG TGTGCAGGCG TTCACTAGCA AGGGTGGAGG TCCCATAAGT 
CCCAGTATCC AGAGCAGGAC CATGTCTACG TCACCTGCAT TTGCCACAAG TTTTGGTGTC 
AAAGCTGTGA TGAAAACCTC TGTCCTTCTC ACCTGGGAGG TGCCAGAAAA TTATAAATCA 
CAAGTGCCTT TTAAGATCCT CTATAACCAG CAGAGTGTGG AAGTCCAGGG GAATCTGAAG 
AGGAAGCTGA TCACACGCCT GCAGCCGGAC ACTGATTACT CGTTTGTGCT GATGAGCCGG 
GGAAACAGCG CCGGTGGCCT ACAACAGCAG GTCTCCATCC GCACTGCACC AGACCTGTTC 
AAAACTAAAC CTGTCCTGTA CACGGCAGAC CAGACCAACA ATGGCAAACT CACCATCAAC 
CTGCCCAAAG TGCCCACCTC TGCTCCTGTC AGGTGTTATT ACATTGTGGT CGTGCCCGTC 
TCTCAGACAT CATCTCGTCA GTGGATTAAT CCCGATGAGA TGGAGCTGGA TGAGCTCCTG 
GAGTCTAGTG AGGGAGCTCA TCTCAGGAGA CGTCGACGGC ACACAGACAC CGTAAGACCG 
TACATCGCCG CAAAGCTATC CACGCTCCCA GAAACCTTCA CTCTGGGCGA TGAGCTAGAC 
TACAGCGGCT TCATCAACCG GCCTCTTCCC AACAACCAAC ACTTCCAGTG TTTTGTGATG 
GCTGAACTCA AGGACCAATA CCCTGTTACT GCCAATGAGA AGCAGCGGAC CTTCTCGGCT 
AGCCCATACT CAGACCCCAT CATAGTGCAG GGTGAAAACC AAATGCAGCG CAGTGTGGAC 
CAACCGGAGA TGCTTTGGGT CATGGGCCCT GTGCTCGCTG TGGTGCTCAT CATCATCATA 
GTCATCGCCA TCCTGCTCTT CAAAAGCAAA CAGGAGAGGA AGAGGGCTTC CCCCCTACCA 
AAAGACGATC ATTCGGGTGG TGTGAAGGAT TGTCTTCTGG CCAGCTCATC CGATCCTGTC 
GAAATGAGGA GACTCAACTA CCAGACACCA GGAATGAGGG AACATCCTCC CATATCCGTC 
TCCGAACTGG CAGACCACAT CGAGCGCCTC AAGGCCAACG ATGCCCTCCG GTTTTCCCAA 
GAGTATGAGT CTATTGATCC AGGCCAGCAG TTCACATGGG AGAACTCCAA CTTGGAGGTC 
AACAAGCCAA AGAACCGTTA TGCCAACGTT ATTGCTTACG ACCACTCGAG AGTTGTTCTC 
ACCCCTGTGG ACGGTGTACC AGGGAGTGAC TACATTAATT CCAACTACAT TGATGGCTAC 
CGTAAGCAGA ATGCCTACAT TGCCACACAA GGTCCTCTGC CTGAGACTCT GAGCGACTTC 
TGGAGGATGA TTTGGGAGCA ACGAGCCAGT ACCATCGTCA TGATGACCAG ACTGGAAGAG 
AAGTCCAGAG TCAAGTGTGA CCAATACTGG CCCATTCGTG GCACAGAGAC TTATGGAATG 
ATTCAAGTGA CCATGCTTGA TGCTGTTGAG CTGGCAACCT ACAGTGTGCG CACCTTTGCC 
CTTTACAAGA ATGGCTCTAG TGAGAAGCGC GAGGTCAGGC AGTTTCAGTT CATGGCTTGG 
CCGGACCACG GAGTGCCTGA ATATCCCACC CCCATCCTAG CCTTCCTGCG CAGGGTCAAA 
GCCTGCAACC CTCCTGATGC TGGACCCATG GTAGTGCACT GCAGTGCTGG AGTTGGCCGT 
ACAGGCTGCT TCATAGTCAT CGATGCGATG TTGGAGCGCA TGAAGCACGA GAAGACCGTA 
GACATTTATG GCCACGTCAC GTGCATGAGA TCCCAGAGGA ACTATATGGT GCAGACGGAG 
GACCAGTACA TCTTTATCCA CGAGGCCCTT CTGGAGGCCG CCACCTGTGG AAACACAGAG 
GTCCCTGCCC GAAACCTCTA CGCCCACATC CAGAAGCTCA CACAGCCATC GGCTGGAGAG 
ACCGTCACAG CCATGGAGCT GGAGTTTAAG AGACTCGCCA ACTCCAAAGC CCACACCTCA 
AGATTCATCA GCGCCAACCT GCCCTGCAAC AAGTTCAAGA ACCGGCTGGT CAATATCATG 
CCTTTCGAGT CCACACGGGT GTGCCTGCAG CCAATCCGAG GAGTGGAGGG GTCCGACTAC 
ATCAATGCCA GCTGCATTGA CGGATACAGA CAGCAGAAGG CCTATATTGC CACTCAGGGT 
CCTCTAGCGG AGACCACCGA GGACTTCTGG AGGATGTTAT GGGAGCACAA TTCCACTATT 
GTAGTGATGC TGACCAAACT CAGAGAGATG GGACGGGAGA AGTGTCATCA GTACTGGCCC 
GCCGAGCGCT CAGCCCGCTA TCAGTACTTC GTAGTGGATC CCATGGCCGA ATACAACATG 
CCCCAGTACA TCCTGCGAGA GTTCAAAGTC ACAGATGCCA GGGACGGTCA ATCCAGAACC 
ATTCGCCAGT TCCAGTTCAC TGATTGGCCA GAGCAGGGAG TGCCAAAAAC CGGTGAGGGG 
TTCATCGATT TCATTGGGCA AGTTCATAAA ACCAAGGAGC AGTTTGGGCA GGATGGACCA 
ATCACTGTGC ACTGCAGTGC TGGAGTGGGA AGAACAGGTG TTTTCATCAC TCTCAGTATT 
GTCCTGGAGA GGATGAGATA TGAGGGGGTG GTGGATCTGT TCCAGACGGT TAAAACCCTG 
CGCACACAAA GGCCTGCCAT GGTGCAGACA GAGGACCAGT ACCAGCTGTG CTACAGAGCC 
GCTCTGGAGT ACCTGGGGAG CTTTGATCAC TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296739.1
CDS	425..6166
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298464.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298464.2

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT CAAGACGTGC ACCTGCTCAG TCTGAGCTCC 
ACCAGCATCA AGGTGAGTTG GGTGGCACCA CCCGCCGCTA GCCGCCACGG CGCAATAGTG 
CGCTACACAG TCAGCTACCA GGCAGTGAAT GGAGAAGACA CAGAGCGCCA TGAGGTGCCG 
GACATTGGAG CGGATGCCTC CAGCTGTGTG TTGGAAGGTC TAGAGAAGTG GACGGAGTAT 
CAGGTGTGGG TGAGAGCGCA CACTGATGTG GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT 
ATCCGAACCA ATGAAGACGT GCCAGGAGCA CCGCCTCGGA AATTGGAAGT GGAGGCTTTG 
AACTCCACCG CTATCCGTGT GACATGGAAA CCTCCTCTTT CTGGAAAGCA GCATGGCCAG 
ATCCGGGGAT ATCAGGTCAT ATACTCCCGG TTGGAGAACG GGGAACCACG GGGACATCCG 
AATATAATGG ATGTCGCTCT GCCAGAAGCA CAGTGGAAAA TTGAAGAGTC TACGGAATAT 
GAGGCTGTGA TCGCTGGACT TGCCTCTGAA ACCTCTTACT CAGTGACTGT GGCTGCGTAC 
ACAACCAAAG GTGATGGAGC TCGCAGCAAA GCCAAAGTGG TCACCACCAC CGGAGCAGTG 
CCTGGCAAAC CCACTATGAT CATCAGCACC ACCGTTGGCA ACACGGCCCT TATCCAGTGG 
CAGCCTCCTA AAGACATGGT GGGTGAGCTG ATGGGCTACC GGCTGCGCTA CAAACGTCTG 
GAAGAGGAAA ACTATGAGAT ACGCGAATTC AGTCGCACCG ACGACCACCA CACCGTCACT 
GACCTGCACA AGGGCGCCAC CTACAGCTTC CACCTGAGCG CCAGAAACCG CGCTGGACTC 
GGAGAGGAGT ACGTTAAAGA CATCGGCACT CCTGAGGACG TGCCCAGCGG GTTTCCTCTT 
AACCTGCAGG TCGTAGGCTT GACTACCTCT ACCACCCGAC TGACCTGGGA GCCTCCAGCG 
TTGTCTGAAC GTAACGGACG GATCATACAT TACATTGTGG TTTACCGAGA CATCAACAGC 
AAACAAAACA GCACCAACCG AACCTCCGAC ACACAGATGA CCATCCAAGG GCTCCGACCT 
GACACCACCT ATGATATCCG TGTGCAGGCG TTCACTAGCA AGGGTGGAGG TCCCATAAGT 
CCCAGTATCC AGAGCAGGAC CATGTCTACG TCACCTGCAT TTGCCACAAG TTTTGGTGTC 
AAAGCTGTGA TGAAAACCTC TGTCCTTCTC ACCTGGGAGG TGCCAGAAAA TTATAAATCA 
CAAGTGCCTT TTAAGATCCT CTATAACCAG CAGAGTGTGG AAGTCCAGGG GAATCTGAAG 
AGGAAGCTGA TCACACGCCT GCAGCCGGAC ACTGATTACT CGTTTGTGCT GATGAGCCGG 
GGAAACAGCG CCGGTGGCCT ACAACAGCAG GTCTCCATCC GCACTGCACC AGACCTGTTC 
AAAACTAAAC CTGTCCTGTA CACGGCAGAC CAGACCAACA ATGGCAAACT CACCATCAAC 
CTGCCCAAAG TGCCCACCTC TGCTCCTGTC AGGTGTTATT ACATTGTGGT CGTGCCCGTC 
TCTCAGACAT CATCTCGTCA GTGGATTAAT CCCGATGAGA TGGAGCTGGA TGAGCTCCTG 
GAGTCTAGTG AGGGAGCTCA TCTCAGGAGA CGTCGACGGC ACACAGACAC CGTAAGACCG 
TACATCGCCG CAAAGCTATC CACGCTCCCA GAAACCTTCA CTCTGGGCGA TGAGCTAGAC 
TACAGCGGCT TCATCAACCG GCCTCTTCCC AACAACCAAC ACTTCCAGTG TTTTGTGATG 
GCTGAACTCA AGGACCAATA CCCTGTTACT GCCAATGAGA AGCAGCGGAC CTTCTCGGCT 
AGCCCATACT CAGACCCCAT CATAGTGCAG GGTGAAAACC AAATGCAGCG CAGTGTGGAC 
CAACCGGAGA TGCTTTGGGT CATGGGCCCT GTGCTCGCTG TGGTGCTCAT CATCATCATA 
GTCATCGCCA TCCTGCTCTT CAAAAGCAAA CAGGAGAGGA AGAGGGCTTC CCCCCTACCA 
AAAGACGATC ATTCGGGTGG TGTGAAGGAT TGTCTTCTGG CCAGCTCATC CGATCCTGTC 
GAAATGAGGA GACTCAACTA CCAGACACCA GGAATGAGGG AACATCCTCC CATATCCGTC 
TCCGAACTGG CAGACCACAT CGAGCGCCTC AAGGCCAACG ATGCCCTCCG GTTTTCCCAA 
GAGTATGAGT CTATTGATCC AGGCCAGCAG TTCACATGGG AGAACTCCAA CTTGGAGGTC 
AACAAGCCAA AGAACCGTTA TGCCAACGTT ATTGCTTACG ACCACTCGAG AGTTGTTCTC 
ACCCCTGTGG ACGGTGTACC AGGGAGTGAC TACATTAATT CCAACTACAT TGATGGCTAC 
CGTAAGCAGA ATGCCTACAT TGCCACACAA GGTCCTCTGC CTGAGACTCT GAGCGACTTC 
TGGAGGATGA TTTGGGAGCA ACGAGCCAGT ACCATCGTCA TGATGACCAG ACTGGAAGAG 
AAGTCCAGAG TCAAGTGTGA CCAATACTGG CCCATTCGTG GCACAGAGAC TTATGGAATG 
ATTCAAGTGA CCATGCTTGA TGCTGTTGAG CTGGCAACCT ACAGTGTGCG CACCTTTGCC 
CTTTACAAGA ATGGCTCTAG TGAGAAGCGC GAGGTCAGGC AGTTTCAGTT CATGGCTTGG 
CCGGACCACG GAGTGCCTGA ATATCCCACC CCCATCCTAG CCTTCCTGCG CAGGGTCAAA 
GCCTGCAACC CTCCTGATGC TGGACCCATG GTAGTGCACT GCAGTGCTGG AGTTGGCCGT 
ACAGGCTGCT TCATAGTCAT CGATGCGATG TTGGAGCGCA TGAAGCACGA GAAGACCGTA 
GACATTTATG GCCACGTCAC GTGCATGAGA TCCCAGAGGA ACTATATGGT GCAGACGGAG 
GACCAGTACA TCTTTATCCA CGAGGCCCTT CTGGAGGCCG CCACCTGTGG AAACACAGAG 
GTCCCTGCCC GAAACCTCTA CGCCCACATC CAGAAGCTCA CACAGCCATC GGCTGGAGAG 
ACCGTCACAG CCATGGAGCT GGAGTTTAAG AGACTCGCCA ACTCCAAAGC CCACACCTCA 
AGATTCATCA GCGCCAACCT GCCCTGCAAC AAGTTCAAGA ACCGGCTGGT CAATATCATG 
CCTTTCGAGT CCACACGGGT GTGCCTGCAG CCAATCCGAG GAGTGGAGGG GTCCGACTAC 
ATCAATGCCA GCTGCATTGA CGGATACAGA CAGCAGAAGG CCTATATTGC CACTCAGGGT 
CCTCTAGCGG AGACCACCGA GGACTTCTGG AGGATGTTAT GGGAGCACAA TTCCACTATT 
GTAGTGATGC TGACCAAACT CAGAGAGATG GGACGGGAGA AGTGTCATCA GTACTGGCCC 
GCCGAGCGCT CAGCCCGCTA TCAGTACTTC GTAGTGGATC CCATGGCCGA ATACAACATG 
CCCCAGTACA TCCTGCGAGA GTTCAAAGTC ACAGATGCCA GGGACGGTCA ATCCAGAACC 
ATTCGCCAGT TCCAGTTCAC TGATTGGCCA GAGCAGGGAG TGCCAAAAAC CGGTGAGGGG 
TTCATCGATT TCATTGGGCA AGTTCATAAA ACCAAGGAGC AGTTTGGGCA GGATGGACCA 
ATCACTGTGC ACTGCAGTGC TGGAGTGGGA AGAACAGGTG TTTTCATCAC TCTCAGTATT 
GTCCTGGAGA GGATGAGATA TGAGGGGGTG GTGGATCTGT TCCAGACGGT TAAAACCCTG 
CGCACACAAA GGCCTGCCAT GGTGCAGACA GAGGACCAGT ACCAGCTGTG CTACAGAGCC 
GCTCTGGAGT ACCTGGGGAG CTTTGATCAC TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298466.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296741.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X11, mRNA.

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CloneID	ODa47420
Clone ID Related Accession (Same CDS sequence)	XM_009298466.3 , XM_009298466.2
Accession Version	XM_009298466.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298466.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT CAAGACGTGC ACCTGCTCAG TCTGAGCTCC 
ACCAGCATCA AGGTGAGTTG GGTGGCACCA CCCGCCGCTA GCCGCCACGG CGCAATAGTG 
CGCTACACAG TCAGCTACCA GGCAGTGAAT GGAGAAGACA CAGAGCGCCA TGAGGTGCCG 
GACATTGGAG CGGATGCCTC CAGCTGTGTG TTGGAAGGTC TAGAGAAGTG GACGGAGTAT 
CAGGTGTGGG TGAGAGCGCA CACTGATGTG GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT 
ATCCGAACCA ATGAAGACGT GCCAGGAGCA CCGCCTCGGA AATTGGAAGT GGAGGCTTTG 
AACTCCACCG CTATCCGTGT GACATGGAAA CCTCCTCTTT CTGGAAAGCA GCATGGCCAG 
ATCCGGGGAT ATCAGGTCAT ATACTCCCGG TTGGAGAACG GGGAACCACG GGGACATCCG 
AATATAATGG ATGTCGCTCT GCCAGAAGCA CAGGAGGCTG TGATCGCTGG ACTTGCCTCT 
GAAACCTCTT ACTCAGTGAC TGTGGCTGCG TACACAACCA AAGGTGATGG AGCTCGCAGC 
AAAGCCAAAG TGGTCACCAC CACCGGAGCA GTGCCTGGCA AACCCACTAT GATCATCAGC 
ACCACCGTTG GCAACACGGC CCTTATCCAG TGGCAGCCTC CTAAAGACAT GGTGGGTGAG 
CTGATGGGCT ACCGGCTGCG CTACAAACGT CTGGAAGAGG AAAACTATGA GATACGCGAA 
TTCAGTCGCA CCGACGACCA CCACACCGTC ACTGACCTGC ACAAGGGCGC CACCTACAGC 
TTCCACCTGA GCGCCAGAAA CCGCGCTGGA CTCGGAGAGG AGTACGTTAA AGACATCGGC 
ACTCCTGAGG ACGTGCCCAG CGGGTTTCCT CTTAACCTGC AGGTCGTAGG CTTGACTACC 
TCTACCACCC GACTGACCTG GGAGCCTCCA GCGTTGTCTG AACGTAACGG ACGGATCATA 
CATTACATTG TGGTTTACCG AGACATCAAC AGCAAACAAA ACAGCACCAA CCGAACCTCC 
GACACACAGA TGACCATCCA AGGGCTCCGA CCTGACACCA CCTATGATAT CCGTGTGCAG 
GCGTTCACTA GCAAGGGTGG AGGTCCCATA AGTCCCAGTA TCCAGAGCAG GACCATGTCT 
ACGTCACCTG CATTTGCCAC AAGTTTTGGT GTCAAAGCTG TGATGAAAAC CTCTGTCCTT 
CTCACCTGGG AGGTGCCAGA AAATTATAAA TCACAAGTGC CTTTTAAGAT CCTCTATAAC 
CAGCAGAGTG TGGAAGTCCA GGGGAATCTG AAGAGGAAGC TGATCACACG CCTGCAGCCG 
GACACTGATT ACTCGTTTGT GCTGATGAGC CGGGGAAACA GCGCCGGTGG CCTACAACAG 
CAGGTCTCCA TCCGCACTGC ACCAGACCTG TTCAAAACTA AACCTGTCCT GTACACGGCA 
GACCAGACCA ACAATGGCAA ACTCACCATC AACCTGCCCA AAGTGCCCAC CTCTGCTCCT 
GTCAGGTGTT ATTACATTGT GGTCGTGCCC GTCTCTCAGA CATCATCTCG TCAGTGGATT 
AATCCCGATG AGATGGAGCT GGATGAGCTC CTGGAGTCTA GTGAGGGAGC TCATCTCAGG 
AGACGTCGAC GGCACACAGA CACCGTAAGA CCGTACATCG CCGCAAAGCT ATCCACGCTC 
CCAGAAACCT TCACTCTGGG CGATGAGCTA GACTACAGCG GCTTCATCAA CCGGCCTCTT 
CCCAACAACC AACACTTCCA GTGTTTTGTG ATGGCTGAAC TCAAGGACCA ATACCCTGTT 
ACTGCCAATG AGAAGCAGCG GACCTTCTCG GCTAGCCCAT ACTCAGACCC CATCATAGTG 
CAGGGTGAAA ACCAAATGCA GCGCAGTGTG GACCAACCGG AGATGCTTTG GGTCATGGGC 
CCTGTGCTCG CTGTGGTGCT CATCATCATC ATAGTCATCG CCATCCTGCT CTTCAAAAGC 
AAACAGGAGA GGAAGAGGGC TTCCCCCCTA CCAAAAGACG ATCATTCGGG TGGTGTGAAG 
GATTGTCTTC TGGCCAGCTC ATCCGATCCT GTCGAAATGA GGAGACTCAA CTACCAGACA 
CCAGGAATGA GGGAACATCC TCCCATATCC GTCTCCGAAC TGGCAGACCA CATCGAGCGC 
CTCAAGGCCA ACGATGCCCT CCGGTTTTCC CAAGAGTATG AGTCTATTGA TCCAGGCCAG 
CAGTTCACAT GGGAGAACTC CAACTTGGAG GTCAACAAGC CAAAGAACCG TTATGCCAAC 
GTTATTGCTT ACGACCACTC GAGAGTTGTT CTCACCCCTG TGGACGGTGT ACCAGGGAGT 
GACTACATTA ATTCCAACTA CATTGATGGC TACCGTAAGC AGAATGCCTA CATTGCCACA 
CAAGGTCCTC TGCCTGAGAC TCTGAGCGAC TTCTGGAGGA TGATTTGGGA GCAACGAGCC 
AGTACCATCG TCATGATGAC CAGACTGGAA GAGAAGTCCA GAGTCAAGTG TGACCAATAC 
TGGCCCATTC GTGGCACAGA GACTTATGGA ATGATTCAAG TGACCATGCT TGATGCTGTT 
GAGCTGGCAA CCTACAGTGT GCGCACCTTT GCCCTTTACA AGAATGGCTC TAGTGAGAAG 
CGCGAGGTCA GGCAGTTTCA GTTCATGGCT TGGCCGGACC ACGGAGTGCC TGAATATCCC 
ACCCCCATCC TAGCCTTCCT GCGCAGGGTC AAAGCCTGCA ACCCTCCTGA TGCTGGACCC 
ATGGTAGTGC ACTGCAGTGC TGGAGTTGGC CGTACAGGCT GCTTCATAGT CATCGATGCG 
ATGTTGGAGC GCATGAAGCA CGAGAAGACC GTAGACATTT ATGGCCACGT CACGTGCATG 
AGATCCCAGA GGAACTATAT GGTGCAGACG GAGGACCAGT ACATCTTTAT CCACGAGGCC 
CTTCTGGAGG CCGCCACCTG TGGAAACACA GAGGTCCCTG CCCGAAACCT CTACGCCCAC 
ATCCAGAAGC TCACACAGCC ATCGGCTGGA GAGACCGTCA CAGCCATGGA GCTGGAGTTT 
AAGAGACTCG CCAACTCCAA AGCCCACACC TCAAGATTCA TCAGCGCCAA CCTGCCCTGC 
AACAAGTTCA AGAACCGGCT GGTCAATATC ATGCCTTTCG AGTCCACACG GGTGTGCCTG 
CAGCCAATCC GAGGAGTGGA GGGGTCCGAC TACATCAATG CCAGCTGCAT TGACGGATAC 
AGACAGCAGA AGGCCTATAT TGCCACTCAG GGTCCTCTAG CGGAGACCAC CGAGGACTTC 
TGGAGGATGT TATGGGAGCA CAATTCCACT ATTGTAGTGA TGCTGACCAA ACTCAGAGAG 
ATGGGACGGG AGAAGTGTCA TCAGTACTGG CCCGCCGAGC GCTCAGCCCG CTATCAGTAC 
TTCGTAGTGG ATCCCATGGC CGAATACAAC ATGCCCCAGT ACATCCTGCG AGAGTTCAAA 
GTCACAGATG CCAGGGACGG TCAATCCAGA ACCATTCGCC AGTTCCAGTT CACTGATTGG 
CCAGAGCAGG GAGTGCCAAA AACCGGTGAG GGGTTCATCG ATTTCATTGG GCAAGTTCAT 
AAAACCAAGG AGCAGTTTGG GCAGGATGGA CCAATCACTG TGCACTGCAG TGCTGGAGTG 
GGAAGAACAG GTGTTTTCAT CACTCTCAGT ATTGTCCTGG AGAGGATGAG ATATGAGGGG 
GTGGTGGATC TGTTCCAGAC GGTTAAAACC CTGCGCACAC AAAGGCCTGC CATGGTGCAG 
ACAGAGGACC AGTACCAGCT GTGCTACAGA GCCGCTCTGG AGTACCTGGG GAGCTTTGAT 
CACTATGCAA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296741.1
CDS	421..6135
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298466.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298466.3

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT CAAGACGTGC ACCTGCTCAG TCTGAGCTCC 
ACCAGCATCA AGGTGAGTTG GGTGGCACCA CCCGCCGCTA GCCGCCACGG CGCAATAGTG 
CGCTACACAG TCAGCTACCA GGCAGTGAAT GGAGAAGACA CAGAGCGCCA TGAGGTGCCG 
GACATTGGAG CGGATGCCTC CAGCTGTGTG TTGGAAGGTC TAGAGAAGTG GACGGAGTAT 
CAGGTGTGGG TGAGAGCGCA CACTGATGTG GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT 
ATCCGAACCA ATGAAGACGT GCCAGGAGCA CCGCCTCGGA AATTGGAAGT GGAGGCTTTG 
AACTCCACCG CTATCCGTGT GACATGGAAA CCTCCTCTTT CTGGAAAGCA GCATGGCCAG 
ATCCGGGGAT ATCAGGTCAT ATACTCCCGG TTGGAGAACG GGGAACCACG GGGACATCCG 
AATATAATGG ATGTCGCTCT GCCAGAAGCA CAGGAGGCTG TGATCGCTGG ACTTGCCTCT 
GAAACCTCTT ACTCAGTGAC TGTGGCTGCG TACACAACCA AAGGTGATGG AGCTCGCAGC 
AAAGCCAAAG TGGTCACCAC CACCGGAGCA GTGCCTGGCA AACCCACTAT GATCATCAGC 
ACCACCGTTG GCAACACGGC CCTTATCCAG TGGCAGCCTC CTAAAGACAT GGTGGGTGAG 
CTGATGGGCT ACCGGCTGCG CTACAAACGT CTGGAAGAGG AAAACTATGA GATACGCGAA 
TTCAGTCGCA CCGACGACCA CCACACCGTC ACTGACCTGC ACAAGGGCGC CACCTACAGC 
TTCCACCTGA GCGCCAGAAA CCGCGCTGGA CTCGGAGAGG AGTACGTTAA AGACATCGGC 
ACTCCTGAGG ACGTGCCCAG CGGGTTTCCT CTTAACCTGC AGGTCGTAGG CTTGACTACC 
TCTACCACCC GACTGACCTG GGAGCCTCCA GCGTTGTCTG AACGTAACGG ACGGATCATA 
CATTACATTG TGGTTTACCG AGACATCAAC AGCAAACAAA ACAGCACCAA CCGAACCTCC 
GACACACAGA TGACCATCCA AGGGCTCCGA CCTGACACCA CCTATGATAT CCGTGTGCAG 
GCGTTCACTA GCAAGGGTGG AGGTCCCATA AGTCCCAGTA TCCAGAGCAG GACCATGTCT 
ACGTCACCTG CATTTGCCAC AAGTTTTGGT GTCAAAGCTG TGATGAAAAC CTCTGTCCTT 
CTCACCTGGG AGGTGCCAGA AAATTATAAA TCACAAGTGC CTTTTAAGAT CCTCTATAAC 
CAGCAGAGTG TGGAAGTCCA GGGGAATCTG AAGAGGAAGC TGATCACACG CCTGCAGCCG 
GACACTGATT ACTCGTTTGT GCTGATGAGC CGGGGAAACA GCGCCGGTGG CCTACAACAG 
CAGGTCTCCA TCCGCACTGC ACCAGACCTG TTCAAAACTA AACCTGTCCT GTACACGGCA 
GACCAGACCA ACAATGGCAA ACTCACCATC AACCTGCCCA AAGTGCCCAC CTCTGCTCCT 
GTCAGGTGTT ATTACATTGT GGTCGTGCCC GTCTCTCAGA CATCATCTCG TCAGTGGATT 
AATCCCGATG AGATGGAGCT GGATGAGCTC CTGGAGTCTA GTGAGGGAGC TCATCTCAGG 
AGACGTCGAC GGCACACAGA CACCGTAAGA CCGTACATCG CCGCAAAGCT ATCCACGCTC 
CCAGAAACCT TCACTCTGGG CGATGAGCTA GACTACAGCG GCTTCATCAA CCGGCCTCTT 
CCCAACAACC AACACTTCCA GTGTTTTGTG ATGGCTGAAC TCAAGGACCA ATACCCTGTT 
ACTGCCAATG AGAAGCAGCG GACCTTCTCG GCTAGCCCAT ACTCAGACCC CATCATAGTG 
CAGGGTGAAA ACCAAATGCA GCGCAGTGTG GACCAACCGG AGATGCTTTG GGTCATGGGC 
CCTGTGCTCG CTGTGGTGCT CATCATCATC ATAGTCATCG CCATCCTGCT CTTCAAAAGC 
AAACAGGAGA GGAAGAGGGC TTCCCCCCTA CCAAAAGACG ATCATTCGGG TGGTGTGAAG 
GATTGTCTTC TGGCCAGCTC ATCCGATCCT GTCGAAATGA GGAGACTCAA CTACCAGACA 
CCAGGAATGA GGGAACATCC TCCCATATCC GTCTCCGAAC TGGCAGACCA CATCGAGCGC 
CTCAAGGCCA ACGATGCCCT CCGGTTTTCC CAAGAGTATG AGTCTATTGA TCCAGGCCAG 
CAGTTCACAT GGGAGAACTC CAACTTGGAG GTCAACAAGC CAAAGAACCG TTATGCCAAC 
GTTATTGCTT ACGACCACTC GAGAGTTGTT CTCACCCCTG TGGACGGTGT ACCAGGGAGT 
GACTACATTA ATTCCAACTA CATTGATGGC TACCGTAAGC AGAATGCCTA CATTGCCACA 
CAAGGTCCTC TGCCTGAGAC TCTGAGCGAC TTCTGGAGGA TGATTTGGGA GCAACGAGCC 
AGTACCATCG TCATGATGAC CAGACTGGAA GAGAAGTCCA GAGTCAAGTG TGACCAATAC 
TGGCCCATTC GTGGCACAGA GACTTATGGA ATGATTCAAG TGACCATGCT TGATGCTGTT 
GAGCTGGCAA CCTACAGTGT GCGCACCTTT GCCCTTTACA AGAATGGCTC TAGTGAGAAG 
CGCGAGGTCA GGCAGTTTCA GTTCATGGCT TGGCCGGACC ACGGAGTGCC TGAATATCCC 
ACCCCCATCC TAGCCTTCCT GCGCAGGGTC AAAGCCTGCA ACCCTCCTGA TGCTGGACCC 
ATGGTAGTGC ACTGCAGTGC TGGAGTTGGC CGTACAGGCT GCTTCATAGT CATCGATGCG 
ATGTTGGAGC GCATGAAGCA CGAGAAGACC GTAGACATTT ATGGCCACGT CACGTGCATG 
AGATCCCAGA GGAACTATAT GGTGCAGACG GAGGACCAGT ACATCTTTAT CCACGAGGCC 
CTTCTGGAGG CCGCCACCTG TGGAAACACA GAGGTCCCTG CCCGAAACCT CTACGCCCAC 
ATCCAGAAGC TCACACAGCC ATCGGCTGGA GAGACCGTCA CAGCCATGGA GCTGGAGTTT 
AAGAGACTCG CCAACTCCAA AGCCCACACC TCAAGATTCA TCAGCGCCAA CCTGCCCTGC 
AACAAGTTCA AGAACCGGCT GGTCAATATC ATGCCTTTCG AGTCCACACG GGTGTGCCTG 
CAGCCAATCC GAGGAGTGGA GGGGTCCGAC TACATCAATG CCAGCTGCAT TGACGGATAC 
AGACAGCAGA AGGCCTATAT TGCCACTCAG GGTCCTCTAG CGGAGACCAC CGAGGACTTC 
TGGAGGATGT TATGGGAGCA CAATTCCACT ATTGTAGTGA TGCTGACCAA ACTCAGAGAG 
ATGGGACGGG AGAAGTGTCA TCAGTACTGG CCCGCCGAGC GCTCAGCCCG CTATCAGTAC 
TTCGTAGTGG ATCCCATGGC CGAATACAAC ATGCCCCAGT ACATCCTGCG AGAGTTCAAA 
GTCACAGATG CCAGGGACGG TCAATCCAGA ACCATTCGCC AGTTCCAGTT CACTGATTGG 
CCAGAGCAGG GAGTGCCAAA AACCGGTGAG GGGTTCATCG ATTTCATTGG GCAAGTTCAT 
AAAACCAAGG AGCAGTTTGG GCAGGATGGA CCAATCACTG TGCACTGCAG TGCTGGAGTG 
GGAAGAACAG GTGTTTTCAT CACTCTCAGT ATTGTCCTGG AGAGGATGAG ATATGAGGGG 
GTGGTGGATC TGTTCCAGAC GGTTAAAACC CTGCGCACAC AAAGGCCTGC CATGGTGCAG 
ACAGAGGACC AGTACCAGCT GTGCTACAGA GCCGCTCTGG AGTACCTGGG GAGCTTTGAT 
CACTATGCAA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298466.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296741.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X9, mRNA.

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CloneID	ODa47420
Clone ID Related Accession (Same CDS sequence)	XM_009298466.3 , XM_009298466.2
Accession Version	XM_009298466.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298466.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT CAAGACGTGC ACCTGCTCAG TCTGAGCTCC 
ACCAGCATCA AGGTGAGTTG GGTGGCACCA CCCGCCGCTA GCCGCCACGG CGCAATAGTG 
CGCTACACAG TCAGCTACCA GGCAGTGAAT GGAGAAGACA CAGAGCGCCA TGAGGTGCCG 
GACATTGGAG CGGATGCCTC CAGCTGTGTG TTGGAAGGTC TAGAGAAGTG GACGGAGTAT 
CAGGTGTGGG TGAGAGCGCA CACTGATGTG GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT 
ATCCGAACCA ATGAAGACGT GCCAGGAGCA CCGCCTCGGA AATTGGAAGT GGAGGCTTTG 
AACTCCACCG CTATCCGTGT GACATGGAAA CCTCCTCTTT CTGGAAAGCA GCATGGCCAG 
ATCCGGGGAT ATCAGGTCAT ATACTCCCGG TTGGAGAACG GGGAACCACG GGGACATCCG 
AATATAATGG ATGTCGCTCT GCCAGAAGCA CAGGAGGCTG TGATCGCTGG ACTTGCCTCT 
GAAACCTCTT ACTCAGTGAC TGTGGCTGCG TACACAACCA AAGGTGATGG AGCTCGCAGC 
AAAGCCAAAG TGGTCACCAC CACCGGAGCA GTGCCTGGCA AACCCACTAT GATCATCAGC 
ACCACCGTTG GCAACACGGC CCTTATCCAG TGGCAGCCTC CTAAAGACAT GGTGGGTGAG 
CTGATGGGCT ACCGGCTGCG CTACAAACGT CTGGAAGAGG AAAACTATGA GATACGCGAA 
TTCAGTCGCA CCGACGACCA CCACACCGTC ACTGACCTGC ACAAGGGCGC CACCTACAGC 
TTCCACCTGA GCGCCAGAAA CCGCGCTGGA CTCGGAGAGG AGTACGTTAA AGACATCGGC 
ACTCCTGAGG ACGTGCCCAG CGGGTTTCCT CTTAACCTGC AGGTCGTAGG CTTGACTACC 
TCTACCACCC GACTGACCTG GGAGCCTCCA GCGTTGTCTG AACGTAACGG ACGGATCATA 
CATTACATTG TGGTTTACCG AGACATCAAC AGCAAACAAA ACAGCACCAA CCGAACCTCC 
GACACACAGA TGACCATCCA AGGGCTCCGA CCTGACACCA CCTATGATAT CCGTGTGCAG 
GCGTTCACTA GCAAGGGTGG AGGTCCCATA AGTCCCAGTA TCCAGAGCAG GACCATGTCT 
ACGTCACCTG CATTTGCCAC AAGTTTTGGT GTCAAAGCTG TGATGAAAAC CTCTGTCCTT 
CTCACCTGGG AGGTGCCAGA AAATTATAAA TCACAAGTGC CTTTTAAGAT CCTCTATAAC 
CAGCAGAGTG TGGAAGTCCA GGGGAATCTG AAGAGGAAGC TGATCACACG CCTGCAGCCG 
GACACTGATT ACTCGTTTGT GCTGATGAGC CGGGGAAACA GCGCCGGTGG CCTACAACAG 
CAGGTCTCCA TCCGCACTGC ACCAGACCTG TTCAAAACTA AACCTGTCCT GTACACGGCA 
GACCAGACCA ACAATGGCAA ACTCACCATC AACCTGCCCA AAGTGCCCAC CTCTGCTCCT 
GTCAGGTGTT ATTACATTGT GGTCGTGCCC GTCTCTCAGA CATCATCTCG TCAGTGGATT 
AATCCCGATG AGATGGAGCT GGATGAGCTC CTGGAGTCTA GTGAGGGAGC TCATCTCAGG 
AGACGTCGAC GGCACACAGA CACCGTAAGA CCGTACATCG CCGCAAAGCT ATCCACGCTC 
CCAGAAACCT TCACTCTGGG CGATGAGCTA GACTACAGCG GCTTCATCAA CCGGCCTCTT 
CCCAACAACC AACACTTCCA GTGTTTTGTG ATGGCTGAAC TCAAGGACCA ATACCCTGTT 
ACTGCCAATG AGAAGCAGCG GACCTTCTCG GCTAGCCCAT ACTCAGACCC CATCATAGTG 
CAGGGTGAAA ACCAAATGCA GCGCAGTGTG GACCAACCGG AGATGCTTTG GGTCATGGGC 
CCTGTGCTCG CTGTGGTGCT CATCATCATC ATAGTCATCG CCATCCTGCT CTTCAAAAGC 
AAACAGGAGA GGAAGAGGGC TTCCCCCCTA CCAAAAGACG ATCATTCGGG TGGTGTGAAG 
GATTGTCTTC TGGCCAGCTC ATCCGATCCT GTCGAAATGA GGAGACTCAA CTACCAGACA 
CCAGGAATGA GGGAACATCC TCCCATATCC GTCTCCGAAC TGGCAGACCA CATCGAGCGC 
CTCAAGGCCA ACGATGCCCT CCGGTTTTCC CAAGAGTATG AGTCTATTGA TCCAGGCCAG 
CAGTTCACAT GGGAGAACTC CAACTTGGAG GTCAACAAGC CAAAGAACCG TTATGCCAAC 
GTTATTGCTT ACGACCACTC GAGAGTTGTT CTCACCCCTG TGGACGGTGT ACCAGGGAGT 
GACTACATTA ATTCCAACTA CATTGATGGC TACCGTAAGC AGAATGCCTA CATTGCCACA 
CAAGGTCCTC TGCCTGAGAC TCTGAGCGAC TTCTGGAGGA TGATTTGGGA GCAACGAGCC 
AGTACCATCG TCATGATGAC CAGACTGGAA GAGAAGTCCA GAGTCAAGTG TGACCAATAC 
TGGCCCATTC GTGGCACAGA GACTTATGGA ATGATTCAAG TGACCATGCT TGATGCTGTT 
GAGCTGGCAA CCTACAGTGT GCGCACCTTT GCCCTTTACA AGAATGGCTC TAGTGAGAAG 
CGCGAGGTCA GGCAGTTTCA GTTCATGGCT TGGCCGGACC ACGGAGTGCC TGAATATCCC 
ACCCCCATCC TAGCCTTCCT GCGCAGGGTC AAAGCCTGCA ACCCTCCTGA TGCTGGACCC 
ATGGTAGTGC ACTGCAGTGC TGGAGTTGGC CGTACAGGCT GCTTCATAGT CATCGATGCG 
ATGTTGGAGC GCATGAAGCA CGAGAAGACC GTAGACATTT ATGGCCACGT CACGTGCATG 
AGATCCCAGA GGAACTATAT GGTGCAGACG GAGGACCAGT ACATCTTTAT CCACGAGGCC 
CTTCTGGAGG CCGCCACCTG TGGAAACACA GAGGTCCCTG CCCGAAACCT CTACGCCCAC 
ATCCAGAAGC TCACACAGCC ATCGGCTGGA GAGACCGTCA CAGCCATGGA GCTGGAGTTT 
AAGAGACTCG CCAACTCCAA AGCCCACACC TCAAGATTCA TCAGCGCCAA CCTGCCCTGC 
AACAAGTTCA AGAACCGGCT GGTCAATATC ATGCCTTTCG AGTCCACACG GGTGTGCCTG 
CAGCCAATCC GAGGAGTGGA GGGGTCCGAC TACATCAATG CCAGCTGCAT TGACGGATAC 
AGACAGCAGA AGGCCTATAT TGCCACTCAG GGTCCTCTAG CGGAGACCAC CGAGGACTTC 
TGGAGGATGT TATGGGAGCA CAATTCCACT ATTGTAGTGA TGCTGACCAA ACTCAGAGAG 
ATGGGACGGG AGAAGTGTCA TCAGTACTGG CCCGCCGAGC GCTCAGCCCG CTATCAGTAC 
TTCGTAGTGG ATCCCATGGC CGAATACAAC ATGCCCCAGT ACATCCTGCG AGAGTTCAAA 
GTCACAGATG CCAGGGACGG TCAATCCAGA ACCATTCGCC AGTTCCAGTT CACTGATTGG 
CCAGAGCAGG GAGTGCCAAA AACCGGTGAG GGGTTCATCG ATTTCATTGG GCAAGTTCAT 
AAAACCAAGG AGCAGTTTGG GCAGGATGGA CCAATCACTG TGCACTGCAG TGCTGGAGTG 
GGAAGAACAG GTGTTTTCAT CACTCTCAGT ATTGTCCTGG AGAGGATGAG ATATGAGGGG 
GTGGTGGATC TGTTCCAGAC GGTTAAAACC CTGCGCACAC AAAGGCCTGC CATGGTGCAG 
ACAGAGGACC AGTACCAGCT GTGCTACAGA GCCGCTCTGG AGTACCTGGG GAGCTTTGAT 
CACTATGCAA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296741.1
CDS	425..6139
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298466.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298466.2

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT CAAGACGTGC ACCTGCTCAG TCTGAGCTCC 
ACCAGCATCA AGGTGAGTTG GGTGGCACCA CCCGCCGCTA GCCGCCACGG CGCAATAGTG 
CGCTACACAG TCAGCTACCA GGCAGTGAAT GGAGAAGACA CAGAGCGCCA TGAGGTGCCG 
GACATTGGAG CGGATGCCTC CAGCTGTGTG TTGGAAGGTC TAGAGAAGTG GACGGAGTAT 
CAGGTGTGGG TGAGAGCGCA CACTGATGTG GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT 
ATCCGAACCA ATGAAGACGT GCCAGGAGCA CCGCCTCGGA AATTGGAAGT GGAGGCTTTG 
AACTCCACCG CTATCCGTGT GACATGGAAA CCTCCTCTTT CTGGAAAGCA GCATGGCCAG 
ATCCGGGGAT ATCAGGTCAT ATACTCCCGG TTGGAGAACG GGGAACCACG GGGACATCCG 
AATATAATGG ATGTCGCTCT GCCAGAAGCA CAGGAGGCTG TGATCGCTGG ACTTGCCTCT 
GAAACCTCTT ACTCAGTGAC TGTGGCTGCG TACACAACCA AAGGTGATGG AGCTCGCAGC 
AAAGCCAAAG TGGTCACCAC CACCGGAGCA GTGCCTGGCA AACCCACTAT GATCATCAGC 
ACCACCGTTG GCAACACGGC CCTTATCCAG TGGCAGCCTC CTAAAGACAT GGTGGGTGAG 
CTGATGGGCT ACCGGCTGCG CTACAAACGT CTGGAAGAGG AAAACTATGA GATACGCGAA 
TTCAGTCGCA CCGACGACCA CCACACCGTC ACTGACCTGC ACAAGGGCGC CACCTACAGC 
TTCCACCTGA GCGCCAGAAA CCGCGCTGGA CTCGGAGAGG AGTACGTTAA AGACATCGGC 
ACTCCTGAGG ACGTGCCCAG CGGGTTTCCT CTTAACCTGC AGGTCGTAGG CTTGACTACC 
TCTACCACCC GACTGACCTG GGAGCCTCCA GCGTTGTCTG AACGTAACGG ACGGATCATA 
CATTACATTG TGGTTTACCG AGACATCAAC AGCAAACAAA ACAGCACCAA CCGAACCTCC 
GACACACAGA TGACCATCCA AGGGCTCCGA CCTGACACCA CCTATGATAT CCGTGTGCAG 
GCGTTCACTA GCAAGGGTGG AGGTCCCATA AGTCCCAGTA TCCAGAGCAG GACCATGTCT 
ACGTCACCTG CATTTGCCAC AAGTTTTGGT GTCAAAGCTG TGATGAAAAC CTCTGTCCTT 
CTCACCTGGG AGGTGCCAGA AAATTATAAA TCACAAGTGC CTTTTAAGAT CCTCTATAAC 
CAGCAGAGTG TGGAAGTCCA GGGGAATCTG AAGAGGAAGC TGATCACACG CCTGCAGCCG 
GACACTGATT ACTCGTTTGT GCTGATGAGC CGGGGAAACA GCGCCGGTGG CCTACAACAG 
CAGGTCTCCA TCCGCACTGC ACCAGACCTG TTCAAAACTA AACCTGTCCT GTACACGGCA 
GACCAGACCA ACAATGGCAA ACTCACCATC AACCTGCCCA AAGTGCCCAC CTCTGCTCCT 
GTCAGGTGTT ATTACATTGT GGTCGTGCCC GTCTCTCAGA CATCATCTCG TCAGTGGATT 
AATCCCGATG AGATGGAGCT GGATGAGCTC CTGGAGTCTA GTGAGGGAGC TCATCTCAGG 
AGACGTCGAC GGCACACAGA CACCGTAAGA CCGTACATCG CCGCAAAGCT ATCCACGCTC 
CCAGAAACCT TCACTCTGGG CGATGAGCTA GACTACAGCG GCTTCATCAA CCGGCCTCTT 
CCCAACAACC AACACTTCCA GTGTTTTGTG ATGGCTGAAC TCAAGGACCA ATACCCTGTT 
ACTGCCAATG AGAAGCAGCG GACCTTCTCG GCTAGCCCAT ACTCAGACCC CATCATAGTG 
CAGGGTGAAA ACCAAATGCA GCGCAGTGTG GACCAACCGG AGATGCTTTG GGTCATGGGC 
CCTGTGCTCG CTGTGGTGCT CATCATCATC ATAGTCATCG CCATCCTGCT CTTCAAAAGC 
AAACAGGAGA GGAAGAGGGC TTCCCCCCTA CCAAAAGACG ATCATTCGGG TGGTGTGAAG 
GATTGTCTTC TGGCCAGCTC ATCCGATCCT GTCGAAATGA GGAGACTCAA CTACCAGACA 
CCAGGAATGA GGGAACATCC TCCCATATCC GTCTCCGAAC TGGCAGACCA CATCGAGCGC 
CTCAAGGCCA ACGATGCCCT CCGGTTTTCC CAAGAGTATG AGTCTATTGA TCCAGGCCAG 
CAGTTCACAT GGGAGAACTC CAACTTGGAG GTCAACAAGC CAAAGAACCG TTATGCCAAC 
GTTATTGCTT ACGACCACTC GAGAGTTGTT CTCACCCCTG TGGACGGTGT ACCAGGGAGT 
GACTACATTA ATTCCAACTA CATTGATGGC TACCGTAAGC AGAATGCCTA CATTGCCACA 
CAAGGTCCTC TGCCTGAGAC TCTGAGCGAC TTCTGGAGGA TGATTTGGGA GCAACGAGCC 
AGTACCATCG TCATGATGAC CAGACTGGAA GAGAAGTCCA GAGTCAAGTG TGACCAATAC 
TGGCCCATTC GTGGCACAGA GACTTATGGA ATGATTCAAG TGACCATGCT TGATGCTGTT 
GAGCTGGCAA CCTACAGTGT GCGCACCTTT GCCCTTTACA AGAATGGCTC TAGTGAGAAG 
CGCGAGGTCA GGCAGTTTCA GTTCATGGCT TGGCCGGACC ACGGAGTGCC TGAATATCCC 
ACCCCCATCC TAGCCTTCCT GCGCAGGGTC AAAGCCTGCA ACCCTCCTGA TGCTGGACCC 
ATGGTAGTGC ACTGCAGTGC TGGAGTTGGC CGTACAGGCT GCTTCATAGT CATCGATGCG 
ATGTTGGAGC GCATGAAGCA CGAGAAGACC GTAGACATTT ATGGCCACGT CACGTGCATG 
AGATCCCAGA GGAACTATAT GGTGCAGACG GAGGACCAGT ACATCTTTAT CCACGAGGCC 
CTTCTGGAGG CCGCCACCTG TGGAAACACA GAGGTCCCTG CCCGAAACCT CTACGCCCAC 
ATCCAGAAGC TCACACAGCC ATCGGCTGGA GAGACCGTCA CAGCCATGGA GCTGGAGTTT 
AAGAGACTCG CCAACTCCAA AGCCCACACC TCAAGATTCA TCAGCGCCAA CCTGCCCTGC 
AACAAGTTCA AGAACCGGCT GGTCAATATC ATGCCTTTCG AGTCCACACG GGTGTGCCTG 
CAGCCAATCC GAGGAGTGGA GGGGTCCGAC TACATCAATG CCAGCTGCAT TGACGGATAC 
AGACAGCAGA AGGCCTATAT TGCCACTCAG GGTCCTCTAG CGGAGACCAC CGAGGACTTC 
TGGAGGATGT TATGGGAGCA CAATTCCACT ATTGTAGTGA TGCTGACCAA ACTCAGAGAG 
ATGGGACGGG AGAAGTGTCA TCAGTACTGG CCCGCCGAGC GCTCAGCCCG CTATCAGTAC 
TTCGTAGTGG ATCCCATGGC CGAATACAAC ATGCCCCAGT ACATCCTGCG AGAGTTCAAA 
GTCACAGATG CCAGGGACGG TCAATCCAGA ACCATTCGCC AGTTCCAGTT CACTGATTGG 
CCAGAGCAGG GAGTGCCAAA AACCGGTGAG GGGTTCATCG ATTTCATTGG GCAAGTTCAT 
AAAACCAAGG AGCAGTTTGG GCAGGATGGA CCAATCACTG TGCACTGCAG TGCTGGAGTG 
GGAAGAACAG GTGTTTTCAT CACTCTCAGT ATTGTCCTGG AGAGGATGAG ATATGAGGGG 
GTGGTGGATC TGTTCCAGAC GGTTAAAACC CTGCGCACAC AAAGGCCTGC CATGGTGCAG 
ACAGAGGACC AGTACCAGCT GTGCTACAGA GCCGCTCTGG AGTACCTGGG GAGCTTTGAT 
CACTATGCAA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298467.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296742.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X12, mRNA.

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CloneID	ODa47421
Clone ID Related Accession (Same CDS sequence)	XM_009298467.3 , XM_009298467.2
Accession Version	XM_009298467.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298467.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT CAAGACGTGC ACCTGCTCAG TCTGAGCTCC 
ACCAGCATCA AGGTGAGTTG GGTGGCACCA CCCGCCGCTA GCCGCCACGG CGCAATAGTG 
CGCTACACAG TCAGCTACCA GGCAGTGAAT GGAGAAGACA CAGAGCGCCA TGAGGTGCCG 
GACATTGGAG CGGATGCCTC CAGCTGTGTG TTGGAAGGTC TAGAGAAGTG GACGGAGTAT 
CAGGTGTGGG TGAGAGCGCA CACTGATGTG GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT 
ATCCGAACCA ATGAAGACGT GCCAGGAGCA CCGCCTCGGA AATTGGAAGT GGAGGCTTTG 
AACTCCACCG CTATCCGTGT GACATGGAAA CCTCCTCTTT CTGGAAAGCA GCATGGCCAG 
ATCCGGGGAT ATCAGGTCAT ATACTCCCGG TTGGAGAACG GGGAACCACG GGGACATCCG 
AATATAATGG ATGTCGCTCT GCCAGAAGCA CAGGAGGCTG TGATCGCTGG ACTTGCCTCT 
GAAACCTCTT ACTCAGTGAC TGTGGCTGCG TACACAACCA AAGGTGATGG AGCTCGCAGC 
AAAGCCAAAG TGGTCACCAC CACCGGAGCA GTGCCTGGCA AACCCACTAT GATCATCAGC 
ACCACCGTTG GCAACACGGC CCTTATCCAG TGGCAGCCTC CTAAAGACAT GGTGGGTGAG 
CTGATGGGCT ACCGGCTGCG CTACAAACGT CTGGAAGAGG AAAACTATGA GATACGCGAA 
TTCAGTCGCA CCGACGACCA CCACACCGTC ACTGACCTGC ACAAGGGCGC CACCTACAGC 
TTCCACCTGA GCGCCAGAAA CCGCGCTGGA CTCGGAGAGG AGTACGTTAA AGACATCGGC 
ACTCCTGAGG ACGTGCCCAG CGGGTTTCCT CTTAACCTGC AGGTCGTAGG CTTGACTACC 
TCTACCACCC GACTGACCTG GGAGCCTCCA GCGTTGTCTG AACGTAACGG ACGGATCATA 
CATTACATTG TGGTTTACCG AGACATCAAC AGCAAACAAA ACAGCACCAA CCGAACCTCC 
GACACACAGA TGACCATCCA AGGGCTCCGA CCTGACACCA CCTATGATAT CCGTGTGCAG 
GCGTTCACTA GCAAGGGTGG AGGTCCCATA AGTCCCAGTA TCCAGAGCAG GACCATGTCT 
ACGTCACCTG CATTTGCCAC AAGTTTTGGT GTCAAAGCTG TGATGAAAAC CTCTGTCCTT 
CTCACCTGGG AGGTGCCAGA AAATTATAAA TCACAAGTGC CTTTTAAGAT CCTCTATAAC 
CAGCAGAGTG TGGAAGTCCA GGGGAATCTG AAGAGGAAGC TGATCACACG CCTGCAGCCG 
GACACTGATT ACTCGTTTGT GCTGATGAGC CGGGGAAACA GCGCCGGTGG CCTACAACAG 
CAGGTCTCCA TCCGCACTGC ACCAGACCTG TTCAAAACTA AACCTGTCCT GTACACGGCA 
GACCAGACCA ACAATGGCAA ACTCACCATC AACCTGCCCA AAGTGCCCAC CTCTGCTCCT 
GTCAGGTGTT ATTACATTGT GGTCGTGCCC GTCTCTCAGA CATCATCTCG TCAGTGGATT 
AATCCCGATG AGATGGAGCT GGATGAGCTC CTGGAGTCTA GTGAGGGAGC TCATCTCAGG 
AGACGTCGAC GGCACACAGA CACCGTAAGA CCGTACATCG CCGCAAAGCT ATCCACGCTC 
CCAGAAACCT TCACTCTGGG CGATGAGCTA GACTACAGCG GCTTCATCAA CCGGCCTCTT 
CCCAACAACC AACACTTCCA GTGTTTTGTG ATGGCTGAAC TCAAGGACCA ATACCCTGTT 
ACTGCCAATG AGAAGCAGCG GACCTTCTCG GCTAGCCCAT ACTCAGACCC CATCATAGTG 
CAGGGTGAAA ACCAAATGCA GCGCAGTGTG GACCAACCGG AGATGCTTTG GGTCATGGGC 
CCTGTGCTCG CTGTGGTGCT CATCATCATC ATAGTCATCG CCATCCTGCT CTTCAAAAGG 
AAGAGGGCTT CCCCCCTACC AAAAGACGAT CATTCGGGTG GTGTGAAGGA TTGTCTTCTG 
GCCAGCTCAT CCGATCCTGT CGAAATGAGG AGACTCAACT ACCAGACACC AGGAATGAGG 
GAACATCCTC CCATATCCGT CTCCGAACTG GCAGACCACA TCGAGCGCCT CAAGGCCAAC 
GATGCCCTCC GGTTTTCCCA AGAGTATGAG TCTATTGATC CAGGCCAGCA GTTCACATGG 
GAGAACTCCA ACTTGGAGGT CAACAAGCCA AAGAACCGTT ATGCCAACGT TATTGCTTAC 
GACCACTCGA GAGTTGTTCT CACCCCTGTG GACGGTGTAC CAGGGAGTGA CTACATTAAT 
TCCAACTACA TTGATGGCTA CCGTAAGCAG AATGCCTACA TTGCCACACA AGGTCCTCTG 
CCTGAGACTC TGAGCGACTT CTGGAGGATG ATTTGGGAGC AACGAGCCAG TACCATCGTC 
ATGATGACCA GACTGGAAGA GAAGTCCAGA GTCAAGTGTG ACCAATACTG GCCCATTCGT 
GGCACAGAGA CTTATGGAAT GATTCAAGTG ACCATGCTTG ATGCTGTTGA GCTGGCAACC 
TACAGTGTGC GCACCTTTGC CCTTTACAAG AATGGCTCTA GTGAGAAGCG CGAGGTCAGG 
CAGTTTCAGT TCATGGCTTG GCCGGACCAC GGAGTGCCTG AATATCCCAC CCCCATCCTA 
GCCTTCCTGC GCAGGGTCAA AGCCTGCAAC CCTCCTGATG CTGGACCCAT GGTAGTGCAC 
TGCAGTGCTG GAGTTGGCCG TACAGGCTGC TTCATAGTCA TCGATGCGAT GTTGGAGCGC 
ATGAAGCACG AGAAGACCGT AGACATTTAT GGCCACGTCA CGTGCATGAG ATCCCAGAGG 
AACTATATGG TGCAGACGGA GGACCAGTAC ATCTTTATCC ACGAGGCCCT TCTGGAGGCC 
GCCACCTGTG GAAACACAGA GGTCCCTGCC CGAAACCTCT ACGCCCACAT CCAGAAGCTC 
ACACAGCCAT CGGCTGGAGA GACCGTCACA GCCATGGAGC TGGAGTTTAA GAGACTCGCC 
AACTCCAAAG CCCACACCTC AAGATTCATC AGCGCCAACC TGCCCTGCAA CAAGTTCAAG 
AACCGGCTGG TCAATATCAT GCCTTTCGAG TCCACACGGG TGTGCCTGCA GCCAATCCGA 
GGAGTGGAGG GGTCCGACTA CATCAATGCC AGCTGCATTG ACGGATACAG ACAGCAGAAG 
GCCTATATTG CCACTCAGGG TCCTCTAGCG GAGACCACCG AGGACTTCTG GAGGATGTTA 
TGGGAGCACA ATTCCACTAT TGTAGTGATG CTGACCAAAC TCAGAGAGAT GGGACGGGAG 
AAGTGTCATC AGTACTGGCC CGCCGAGCGC TCAGCCCGCT ATCAGTACTT CGTAGTGGAT 
CCCATGGCCG AATACAACAT GCCCCAGTAC ATCCTGCGAG AGTTCAAAGT CACAGATGCC 
AGGGACGGTC AATCCAGAAC CATTCGCCAG TTCCAGTTCA CTGATTGGCC AGAGCAGGGA 
GTGCCAAAAA CCGGTGAGGG GTTCATCGAT TTCATTGGGC AAGTTCATAA AACCAAGGAG 
CAGTTTGGGC AGGATGGACC AATCACTGTG CACTGCAGTG CTGGAGTGGG AAGAACAGGT 
GTTTTCATCA CTCTCAGTAT TGTCCTGGAG AGGATGAGAT ATGAGGGGGT GGTGGATCTG 
TTCCAGACGG TTAAAACCCT GCGCACACAA AGGCCTGCCA TGGTGCAGAC AGAGGACCAG 
TACCAGCTGT GCTACAGAGC CGCTCTGGAG TACCTGGGGA GCTTTGATCA CTATGCAACG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296742.1
CDS	421..6123
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKRKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298467.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298467.3

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT CAAGACGTGC ACCTGCTCAG TCTGAGCTCC 
ACCAGCATCA AGGTGAGTTG GGTGGCACCA CCCGCCGCTA GCCGCCACGG CGCAATAGTG 
CGCTACACAG TCAGCTACCA GGCAGTGAAT GGAGAAGACA CAGAGCGCCA TGAGGTGCCG 
GACATTGGAG CGGATGCCTC CAGCTGTGTG TTGGAAGGTC TAGAGAAGTG GACGGAGTAT 
CAGGTGTGGG TGAGAGCGCA CACTGATGTG GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT 
ATCCGAACCA ATGAAGACGT GCCAGGAGCA CCGCCTCGGA AATTGGAAGT GGAGGCTTTG 
AACTCCACCG CTATCCGTGT GACATGGAAA CCTCCTCTTT CTGGAAAGCA GCATGGCCAG 
ATCCGGGGAT ATCAGGTCAT ATACTCCCGG TTGGAGAACG GGGAACCACG GGGACATCCG 
AATATAATGG ATGTCGCTCT GCCAGAAGCA CAGGAGGCTG TGATCGCTGG ACTTGCCTCT 
GAAACCTCTT ACTCAGTGAC TGTGGCTGCG TACACAACCA AAGGTGATGG AGCTCGCAGC 
AAAGCCAAAG TGGTCACCAC CACCGGAGCA GTGCCTGGCA AACCCACTAT GATCATCAGC 
ACCACCGTTG GCAACACGGC CCTTATCCAG TGGCAGCCTC CTAAAGACAT GGTGGGTGAG 
CTGATGGGCT ACCGGCTGCG CTACAAACGT CTGGAAGAGG AAAACTATGA GATACGCGAA 
TTCAGTCGCA CCGACGACCA CCACACCGTC ACTGACCTGC ACAAGGGCGC CACCTACAGC 
TTCCACCTGA GCGCCAGAAA CCGCGCTGGA CTCGGAGAGG AGTACGTTAA AGACATCGGC 
ACTCCTGAGG ACGTGCCCAG CGGGTTTCCT CTTAACCTGC AGGTCGTAGG CTTGACTACC 
TCTACCACCC GACTGACCTG GGAGCCTCCA GCGTTGTCTG AACGTAACGG ACGGATCATA 
CATTACATTG TGGTTTACCG AGACATCAAC AGCAAACAAA ACAGCACCAA CCGAACCTCC 
GACACACAGA TGACCATCCA AGGGCTCCGA CCTGACACCA CCTATGATAT CCGTGTGCAG 
GCGTTCACTA GCAAGGGTGG AGGTCCCATA AGTCCCAGTA TCCAGAGCAG GACCATGTCT 
ACGTCACCTG CATTTGCCAC AAGTTTTGGT GTCAAAGCTG TGATGAAAAC CTCTGTCCTT 
CTCACCTGGG AGGTGCCAGA AAATTATAAA TCACAAGTGC CTTTTAAGAT CCTCTATAAC 
CAGCAGAGTG TGGAAGTCCA GGGGAATCTG AAGAGGAAGC TGATCACACG CCTGCAGCCG 
GACACTGATT ACTCGTTTGT GCTGATGAGC CGGGGAAACA GCGCCGGTGG CCTACAACAG 
CAGGTCTCCA TCCGCACTGC ACCAGACCTG TTCAAAACTA AACCTGTCCT GTACACGGCA 
GACCAGACCA ACAATGGCAA ACTCACCATC AACCTGCCCA AAGTGCCCAC CTCTGCTCCT 
GTCAGGTGTT ATTACATTGT GGTCGTGCCC GTCTCTCAGA CATCATCTCG TCAGTGGATT 
AATCCCGATG AGATGGAGCT GGATGAGCTC CTGGAGTCTA GTGAGGGAGC TCATCTCAGG 
AGACGTCGAC GGCACACAGA CACCGTAAGA CCGTACATCG CCGCAAAGCT ATCCACGCTC 
CCAGAAACCT TCACTCTGGG CGATGAGCTA GACTACAGCG GCTTCATCAA CCGGCCTCTT 
CCCAACAACC AACACTTCCA GTGTTTTGTG ATGGCTGAAC TCAAGGACCA ATACCCTGTT 
ACTGCCAATG AGAAGCAGCG GACCTTCTCG GCTAGCCCAT ACTCAGACCC CATCATAGTG 
CAGGGTGAAA ACCAAATGCA GCGCAGTGTG GACCAACCGG AGATGCTTTG GGTCATGGGC 
CCTGTGCTCG CTGTGGTGCT CATCATCATC ATAGTCATCG CCATCCTGCT CTTCAAAAGG 
AAGAGGGCTT CCCCCCTACC AAAAGACGAT CATTCGGGTG GTGTGAAGGA TTGTCTTCTG 
GCCAGCTCAT CCGATCCTGT CGAAATGAGG AGACTCAACT ACCAGACACC AGGAATGAGG 
GAACATCCTC CCATATCCGT CTCCGAACTG GCAGACCACA TCGAGCGCCT CAAGGCCAAC 
GATGCCCTCC GGTTTTCCCA AGAGTATGAG TCTATTGATC CAGGCCAGCA GTTCACATGG 
GAGAACTCCA ACTTGGAGGT CAACAAGCCA AAGAACCGTT ATGCCAACGT TATTGCTTAC 
GACCACTCGA GAGTTGTTCT CACCCCTGTG GACGGTGTAC CAGGGAGTGA CTACATTAAT 
TCCAACTACA TTGATGGCTA CCGTAAGCAG AATGCCTACA TTGCCACACA AGGTCCTCTG 
CCTGAGACTC TGAGCGACTT CTGGAGGATG ATTTGGGAGC AACGAGCCAG TACCATCGTC 
ATGATGACCA GACTGGAAGA GAAGTCCAGA GTCAAGTGTG ACCAATACTG GCCCATTCGT 
GGCACAGAGA CTTATGGAAT GATTCAAGTG ACCATGCTTG ATGCTGTTGA GCTGGCAACC 
TACAGTGTGC GCACCTTTGC CCTTTACAAG AATGGCTCTA GTGAGAAGCG CGAGGTCAGG 
CAGTTTCAGT TCATGGCTTG GCCGGACCAC GGAGTGCCTG AATATCCCAC CCCCATCCTA 
GCCTTCCTGC GCAGGGTCAA AGCCTGCAAC CCTCCTGATG CTGGACCCAT GGTAGTGCAC 
TGCAGTGCTG GAGTTGGCCG TACAGGCTGC TTCATAGTCA TCGATGCGAT GTTGGAGCGC 
ATGAAGCACG AGAAGACCGT AGACATTTAT GGCCACGTCA CGTGCATGAG ATCCCAGAGG 
AACTATATGG TGCAGACGGA GGACCAGTAC ATCTTTATCC ACGAGGCCCT TCTGGAGGCC 
GCCACCTGTG GAAACACAGA GGTCCCTGCC CGAAACCTCT ACGCCCACAT CCAGAAGCTC 
ACACAGCCAT CGGCTGGAGA GACCGTCACA GCCATGGAGC TGGAGTTTAA GAGACTCGCC 
AACTCCAAAG CCCACACCTC AAGATTCATC AGCGCCAACC TGCCCTGCAA CAAGTTCAAG 
AACCGGCTGG TCAATATCAT GCCTTTCGAG TCCACACGGG TGTGCCTGCA GCCAATCCGA 
GGAGTGGAGG GGTCCGACTA CATCAATGCC AGCTGCATTG ACGGATACAG ACAGCAGAAG 
GCCTATATTG CCACTCAGGG TCCTCTAGCG GAGACCACCG AGGACTTCTG GAGGATGTTA 
TGGGAGCACA ATTCCACTAT TGTAGTGATG CTGACCAAAC TCAGAGAGAT GGGACGGGAG 
AAGTGTCATC AGTACTGGCC CGCCGAGCGC TCAGCCCGCT ATCAGTACTT CGTAGTGGAT 
CCCATGGCCG AATACAACAT GCCCCAGTAC ATCCTGCGAG AGTTCAAAGT CACAGATGCC 
AGGGACGGTC AATCCAGAAC CATTCGCCAG TTCCAGTTCA CTGATTGGCC AGAGCAGGGA 
GTGCCAAAAA CCGGTGAGGG GTTCATCGAT TTCATTGGGC AAGTTCATAA AACCAAGGAG 
CAGTTTGGGC AGGATGGACC AATCACTGTG CACTGCAGTG CTGGAGTGGG AAGAACAGGT 
GTTTTCATCA CTCTCAGTAT TGTCCTGGAG AGGATGAGAT ATGAGGGGGT GGTGGATCTG 
TTCCAGACGG TTAAAACCCT GCGCACACAA AGGCCTGCCA TGGTGCAGAC AGAGGACCAG 
TACCAGCTGT GCTACAGAGC CGCTCTGGAG TACCTGGGGA GCTTTGATCA CTATGCAACG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298467.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296742.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X10, mRNA.

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CloneID	ODa47421
Clone ID Related Accession (Same CDS sequence)	XM_009298467.3 , XM_009298467.2
Accession Version	XM_009298467.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298467.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT CAAGACGTGC ACCTGCTCAG TCTGAGCTCC 
ACCAGCATCA AGGTGAGTTG GGTGGCACCA CCCGCCGCTA GCCGCCACGG CGCAATAGTG 
CGCTACACAG TCAGCTACCA GGCAGTGAAT GGAGAAGACA CAGAGCGCCA TGAGGTGCCG 
GACATTGGAG CGGATGCCTC CAGCTGTGTG TTGGAAGGTC TAGAGAAGTG GACGGAGTAT 
CAGGTGTGGG TGAGAGCGCA CACTGATGTG GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT 
ATCCGAACCA ATGAAGACGT GCCAGGAGCA CCGCCTCGGA AATTGGAAGT GGAGGCTTTG 
AACTCCACCG CTATCCGTGT GACATGGAAA CCTCCTCTTT CTGGAAAGCA GCATGGCCAG 
ATCCGGGGAT ATCAGGTCAT ATACTCCCGG TTGGAGAACG GGGAACCACG GGGACATCCG 
AATATAATGG ATGTCGCTCT GCCAGAAGCA CAGGAGGCTG TGATCGCTGG ACTTGCCTCT 
GAAACCTCTT ACTCAGTGAC TGTGGCTGCG TACACAACCA AAGGTGATGG AGCTCGCAGC 
AAAGCCAAAG TGGTCACCAC CACCGGAGCA GTGCCTGGCA AACCCACTAT GATCATCAGC 
ACCACCGTTG GCAACACGGC CCTTATCCAG TGGCAGCCTC CTAAAGACAT GGTGGGTGAG 
CTGATGGGCT ACCGGCTGCG CTACAAACGT CTGGAAGAGG AAAACTATGA GATACGCGAA 
TTCAGTCGCA CCGACGACCA CCACACCGTC ACTGACCTGC ACAAGGGCGC CACCTACAGC 
TTCCACCTGA GCGCCAGAAA CCGCGCTGGA CTCGGAGAGG AGTACGTTAA AGACATCGGC 
ACTCCTGAGG ACGTGCCCAG CGGGTTTCCT CTTAACCTGC AGGTCGTAGG CTTGACTACC 
TCTACCACCC GACTGACCTG GGAGCCTCCA GCGTTGTCTG AACGTAACGG ACGGATCATA 
CATTACATTG TGGTTTACCG AGACATCAAC AGCAAACAAA ACAGCACCAA CCGAACCTCC 
GACACACAGA TGACCATCCA AGGGCTCCGA CCTGACACCA CCTATGATAT CCGTGTGCAG 
GCGTTCACTA GCAAGGGTGG AGGTCCCATA AGTCCCAGTA TCCAGAGCAG GACCATGTCT 
ACGTCACCTG CATTTGCCAC AAGTTTTGGT GTCAAAGCTG TGATGAAAAC CTCTGTCCTT 
CTCACCTGGG AGGTGCCAGA AAATTATAAA TCACAAGTGC CTTTTAAGAT CCTCTATAAC 
CAGCAGAGTG TGGAAGTCCA GGGGAATCTG AAGAGGAAGC TGATCACACG CCTGCAGCCG 
GACACTGATT ACTCGTTTGT GCTGATGAGC CGGGGAAACA GCGCCGGTGG CCTACAACAG 
CAGGTCTCCA TCCGCACTGC ACCAGACCTG TTCAAAACTA AACCTGTCCT GTACACGGCA 
GACCAGACCA ACAATGGCAA ACTCACCATC AACCTGCCCA AAGTGCCCAC CTCTGCTCCT 
GTCAGGTGTT ATTACATTGT GGTCGTGCCC GTCTCTCAGA CATCATCTCG TCAGTGGATT 
AATCCCGATG AGATGGAGCT GGATGAGCTC CTGGAGTCTA GTGAGGGAGC TCATCTCAGG 
AGACGTCGAC GGCACACAGA CACCGTAAGA CCGTACATCG CCGCAAAGCT ATCCACGCTC 
CCAGAAACCT TCACTCTGGG CGATGAGCTA GACTACAGCG GCTTCATCAA CCGGCCTCTT 
CCCAACAACC AACACTTCCA GTGTTTTGTG ATGGCTGAAC TCAAGGACCA ATACCCTGTT 
ACTGCCAATG AGAAGCAGCG GACCTTCTCG GCTAGCCCAT ACTCAGACCC CATCATAGTG 
CAGGGTGAAA ACCAAATGCA GCGCAGTGTG GACCAACCGG AGATGCTTTG GGTCATGGGC 
CCTGTGCTCG CTGTGGTGCT CATCATCATC ATAGTCATCG CCATCCTGCT CTTCAAAAGG 
AAGAGGGCTT CCCCCCTACC AAAAGACGAT CATTCGGGTG GTGTGAAGGA TTGTCTTCTG 
GCCAGCTCAT CCGATCCTGT CGAAATGAGG AGACTCAACT ACCAGACACC AGGAATGAGG 
GAACATCCTC CCATATCCGT CTCCGAACTG GCAGACCACA TCGAGCGCCT CAAGGCCAAC 
GATGCCCTCC GGTTTTCCCA AGAGTATGAG TCTATTGATC CAGGCCAGCA GTTCACATGG 
GAGAACTCCA ACTTGGAGGT CAACAAGCCA AAGAACCGTT ATGCCAACGT TATTGCTTAC 
GACCACTCGA GAGTTGTTCT CACCCCTGTG GACGGTGTAC CAGGGAGTGA CTACATTAAT 
TCCAACTACA TTGATGGCTA CCGTAAGCAG AATGCCTACA TTGCCACACA AGGTCCTCTG 
CCTGAGACTC TGAGCGACTT CTGGAGGATG ATTTGGGAGC AACGAGCCAG TACCATCGTC 
ATGATGACCA GACTGGAAGA GAAGTCCAGA GTCAAGTGTG ACCAATACTG GCCCATTCGT 
GGCACAGAGA CTTATGGAAT GATTCAAGTG ACCATGCTTG ATGCTGTTGA GCTGGCAACC 
TACAGTGTGC GCACCTTTGC CCTTTACAAG AATGGCTCTA GTGAGAAGCG CGAGGTCAGG 
CAGTTTCAGT TCATGGCTTG GCCGGACCAC GGAGTGCCTG AATATCCCAC CCCCATCCTA 
GCCTTCCTGC GCAGGGTCAA AGCCTGCAAC CCTCCTGATG CTGGACCCAT GGTAGTGCAC 
TGCAGTGCTG GAGTTGGCCG TACAGGCTGC TTCATAGTCA TCGATGCGAT GTTGGAGCGC 
ATGAAGCACG AGAAGACCGT AGACATTTAT GGCCACGTCA CGTGCATGAG ATCCCAGAGG 
AACTATATGG TGCAGACGGA GGACCAGTAC ATCTTTATCC ACGAGGCCCT TCTGGAGGCC 
GCCACCTGTG GAAACACAGA GGTCCCTGCC CGAAACCTCT ACGCCCACAT CCAGAAGCTC 
ACACAGCCAT CGGCTGGAGA GACCGTCACA GCCATGGAGC TGGAGTTTAA GAGACTCGCC 
AACTCCAAAG CCCACACCTC AAGATTCATC AGCGCCAACC TGCCCTGCAA CAAGTTCAAG 
AACCGGCTGG TCAATATCAT GCCTTTCGAG TCCACACGGG TGTGCCTGCA GCCAATCCGA 
GGAGTGGAGG GGTCCGACTA CATCAATGCC AGCTGCATTG ACGGATACAG ACAGCAGAAG 
GCCTATATTG CCACTCAGGG TCCTCTAGCG GAGACCACCG AGGACTTCTG GAGGATGTTA 
TGGGAGCACA ATTCCACTAT TGTAGTGATG CTGACCAAAC TCAGAGAGAT GGGACGGGAG 
AAGTGTCATC AGTACTGGCC CGCCGAGCGC TCAGCCCGCT ATCAGTACTT CGTAGTGGAT 
CCCATGGCCG AATACAACAT GCCCCAGTAC ATCCTGCGAG AGTTCAAAGT CACAGATGCC 
AGGGACGGTC AATCCAGAAC CATTCGCCAG TTCCAGTTCA CTGATTGGCC AGAGCAGGGA 
GTGCCAAAAA CCGGTGAGGG GTTCATCGAT TTCATTGGGC AAGTTCATAA AACCAAGGAG 
CAGTTTGGGC AGGATGGACC AATCACTGTG CACTGCAGTG CTGGAGTGGG AAGAACAGGT 
GTTTTCATCA CTCTCAGTAT TGTCCTGGAG AGGATGAGAT ATGAGGGGGT GGTGGATCTG 
TTCCAGACGG TTAAAACCCT GCGCACACAA AGGCCTGCCA TGGTGCAGAC AGAGGACCAG 
TACCAGCTGT GCTACAGAGC CGCTCTGGAG TACCTGGGGA GCTTTGATCA CTATGCAACG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296742.1
CDS	425..6127
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSAPSAPPQDVHLLSLSSTSIKVSWVAPPAASRHGAIVRYTVSYQAVNGEDTERHEVPDIGADASSCVLEGLEKWTEYQVWVRAHTDVGPGPESTAVRIRTNEDVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKRKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298467.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298467.2

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGCCCCCTC TGCCCCCCCT CAAGACGTGC ACCTGCTCAG TCTGAGCTCC 
ACCAGCATCA AGGTGAGTTG GGTGGCACCA CCCGCCGCTA GCCGCCACGG CGCAATAGTG 
CGCTACACAG TCAGCTACCA GGCAGTGAAT GGAGAAGACA CAGAGCGCCA TGAGGTGCCG 
GACATTGGAG CGGATGCCTC CAGCTGTGTG TTGGAAGGTC TAGAGAAGTG GACGGAGTAT 
CAGGTGTGGG TGAGAGCGCA CACTGATGTG GGCCCCGGCC CCGAGAGCAC GGCTGTGCGT 
ATCCGAACCA ATGAAGACGT GCCAGGAGCA CCGCCTCGGA AATTGGAAGT GGAGGCTTTG 
AACTCCACCG CTATCCGTGT GACATGGAAA CCTCCTCTTT CTGGAAAGCA GCATGGCCAG 
ATCCGGGGAT ATCAGGTCAT ATACTCCCGG TTGGAGAACG GGGAACCACG GGGACATCCG 
AATATAATGG ATGTCGCTCT GCCAGAAGCA CAGGAGGCTG TGATCGCTGG ACTTGCCTCT 
GAAACCTCTT ACTCAGTGAC TGTGGCTGCG TACACAACCA AAGGTGATGG AGCTCGCAGC 
AAAGCCAAAG TGGTCACCAC CACCGGAGCA GTGCCTGGCA AACCCACTAT GATCATCAGC 
ACCACCGTTG GCAACACGGC CCTTATCCAG TGGCAGCCTC CTAAAGACAT GGTGGGTGAG 
CTGATGGGCT ACCGGCTGCG CTACAAACGT CTGGAAGAGG AAAACTATGA GATACGCGAA 
TTCAGTCGCA CCGACGACCA CCACACCGTC ACTGACCTGC ACAAGGGCGC CACCTACAGC 
TTCCACCTGA GCGCCAGAAA CCGCGCTGGA CTCGGAGAGG AGTACGTTAA AGACATCGGC 
ACTCCTGAGG ACGTGCCCAG CGGGTTTCCT CTTAACCTGC AGGTCGTAGG CTTGACTACC 
TCTACCACCC GACTGACCTG GGAGCCTCCA GCGTTGTCTG AACGTAACGG ACGGATCATA 
CATTACATTG TGGTTTACCG AGACATCAAC AGCAAACAAA ACAGCACCAA CCGAACCTCC 
GACACACAGA TGACCATCCA AGGGCTCCGA CCTGACACCA CCTATGATAT CCGTGTGCAG 
GCGTTCACTA GCAAGGGTGG AGGTCCCATA AGTCCCAGTA TCCAGAGCAG GACCATGTCT 
ACGTCACCTG CATTTGCCAC AAGTTTTGGT GTCAAAGCTG TGATGAAAAC CTCTGTCCTT 
CTCACCTGGG AGGTGCCAGA AAATTATAAA TCACAAGTGC CTTTTAAGAT CCTCTATAAC 
CAGCAGAGTG TGGAAGTCCA GGGGAATCTG AAGAGGAAGC TGATCACACG CCTGCAGCCG 
GACACTGATT ACTCGTTTGT GCTGATGAGC CGGGGAAACA GCGCCGGTGG CCTACAACAG 
CAGGTCTCCA TCCGCACTGC ACCAGACCTG TTCAAAACTA AACCTGTCCT GTACACGGCA 
GACCAGACCA ACAATGGCAA ACTCACCATC AACCTGCCCA AAGTGCCCAC CTCTGCTCCT 
GTCAGGTGTT ATTACATTGT GGTCGTGCCC GTCTCTCAGA CATCATCTCG TCAGTGGATT 
AATCCCGATG AGATGGAGCT GGATGAGCTC CTGGAGTCTA GTGAGGGAGC TCATCTCAGG 
AGACGTCGAC GGCACACAGA CACCGTAAGA CCGTACATCG CCGCAAAGCT ATCCACGCTC 
CCAGAAACCT TCACTCTGGG CGATGAGCTA GACTACAGCG GCTTCATCAA CCGGCCTCTT 
CCCAACAACC AACACTTCCA GTGTTTTGTG ATGGCTGAAC TCAAGGACCA ATACCCTGTT 
ACTGCCAATG AGAAGCAGCG GACCTTCTCG GCTAGCCCAT ACTCAGACCC CATCATAGTG 
CAGGGTGAAA ACCAAATGCA GCGCAGTGTG GACCAACCGG AGATGCTTTG GGTCATGGGC 
CCTGTGCTCG CTGTGGTGCT CATCATCATC ATAGTCATCG CCATCCTGCT CTTCAAAAGG 
AAGAGGGCTT CCCCCCTACC AAAAGACGAT CATTCGGGTG GTGTGAAGGA TTGTCTTCTG 
GCCAGCTCAT CCGATCCTGT CGAAATGAGG AGACTCAACT ACCAGACACC AGGAATGAGG 
GAACATCCTC CCATATCCGT CTCCGAACTG GCAGACCACA TCGAGCGCCT CAAGGCCAAC 
GATGCCCTCC GGTTTTCCCA AGAGTATGAG TCTATTGATC CAGGCCAGCA GTTCACATGG 
GAGAACTCCA ACTTGGAGGT CAACAAGCCA AAGAACCGTT ATGCCAACGT TATTGCTTAC 
GACCACTCGA GAGTTGTTCT CACCCCTGTG GACGGTGTAC CAGGGAGTGA CTACATTAAT 
TCCAACTACA TTGATGGCTA CCGTAAGCAG AATGCCTACA TTGCCACACA AGGTCCTCTG 
CCTGAGACTC TGAGCGACTT CTGGAGGATG ATTTGGGAGC AACGAGCCAG TACCATCGTC 
ATGATGACCA GACTGGAAGA GAAGTCCAGA GTCAAGTGTG ACCAATACTG GCCCATTCGT 
GGCACAGAGA CTTATGGAAT GATTCAAGTG ACCATGCTTG ATGCTGTTGA GCTGGCAACC 
TACAGTGTGC GCACCTTTGC CCTTTACAAG AATGGCTCTA GTGAGAAGCG CGAGGTCAGG 
CAGTTTCAGT TCATGGCTTG GCCGGACCAC GGAGTGCCTG AATATCCCAC CCCCATCCTA 
GCCTTCCTGC GCAGGGTCAA AGCCTGCAAC CCTCCTGATG CTGGACCCAT GGTAGTGCAC 
TGCAGTGCTG GAGTTGGCCG TACAGGCTGC TTCATAGTCA TCGATGCGAT GTTGGAGCGC 
ATGAAGCACG AGAAGACCGT AGACATTTAT GGCCACGTCA CGTGCATGAG ATCCCAGAGG 
AACTATATGG TGCAGACGGA GGACCAGTAC ATCTTTATCC ACGAGGCCCT TCTGGAGGCC 
GCCACCTGTG GAAACACAGA GGTCCCTGCC CGAAACCTCT ACGCCCACAT CCAGAAGCTC 
ACACAGCCAT CGGCTGGAGA GACCGTCACA GCCATGGAGC TGGAGTTTAA GAGACTCGCC 
AACTCCAAAG CCCACACCTC AAGATTCATC AGCGCCAACC TGCCCTGCAA CAAGTTCAAG 
AACCGGCTGG TCAATATCAT GCCTTTCGAG TCCACACGGG TGTGCCTGCA GCCAATCCGA 
GGAGTGGAGG GGTCCGACTA CATCAATGCC AGCTGCATTG ACGGATACAG ACAGCAGAAG 
GCCTATATTG CCACTCAGGG TCCTCTAGCG GAGACCACCG AGGACTTCTG GAGGATGTTA 
TGGGAGCACA ATTCCACTAT TGTAGTGATG CTGACCAAAC TCAGAGAGAT GGGACGGGAG 
AAGTGTCATC AGTACTGGCC CGCCGAGCGC TCAGCCCGCT ATCAGTACTT CGTAGTGGAT 
CCCATGGCCG AATACAACAT GCCCCAGTAC ATCCTGCGAG AGTTCAAAGT CACAGATGCC 
AGGGACGGTC AATCCAGAAC CATTCGCCAG TTCCAGTTCA CTGATTGGCC AGAGCAGGGA 
GTGCCAAAAA CCGGTGAGGG GTTCATCGAT TTCATTGGGC AAGTTCATAA AACCAAGGAG 
CAGTTTGGGC AGGATGGACC AATCACTGTG CACTGCAGTG CTGGAGTGGG AAGAACAGGT 
GTTTTCATCA CTCTCAGTAT TGTCCTGGAG AGGATGAGAT ATGAGGGGGT GGTGGATCTG 
TTCCAGACGG TTAAAACCCT GCGCACACAA AGGCCTGCCA TGGTGCAGAC AGAGGACCAG 
TACCAGCTGT GCTACAGAGC CGCTCTGGAG TACCTGGGGA GCTTTGATCA CTATGCAACG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298468.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296743.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X11, mRNA.

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CloneID	ODa47422
Clone ID Related Accession (Same CDS sequence)	XM_009298468.2 , XM_009298468.3
Accession Version	XM_009298468.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGTGCCA 
GGAGCACCGC CTCGGAAATT GGAAGTGGAG GCTTTGAACT CCACCGCTAT CCGTGTGACA 
TGGAAACCTC CTCTTTCTGG AAAGCAGCAT GGCCAGATCC GGGGATATCA GGTCATATAC 
TCCCGGTTGG AGAACGGGGA ACCACGGGGA CATCCGAATA TAATGGATGT CGCTCTGCCA 
GAAGCACAGT GGAAAATTGA AGAGTCTACG GAATATGAGG CTGTGATCGC TGGACTTGCC 
TCTGAAACCT CTTACTCAGT GACTGTGGCT GCGTACACAA CCAAAGGTGA TGGAGCTCGC 
AGCAAAGCCA AAGTGGTCAC CACCACCGGA GCAGTGCCTG GCAAACCCAC TATGATCATC 
AGCACCACCG TTGGCAACAC GGCCCTTATC CAGTGGCAGC CTCCTAAAGA CATGGTGGGT 
GAGCTGATGG GCTACCGGCT GCGCTACAAA CGTCTGGAAG AGGAAAACTA TGAGATACGC 
GAATTCAGTC GCACCGACGA CCACCACACC GTCACTGACC TGCACAAGGG CGCCACCTAC 
AGCTTCCACC TGAGCGCCAG AAACCGCGCT GGACTCGGAG AGGAGTACGT TAAAGACATC 
GGCACTCCTG AGGACGTGCC CAGCGGGTTT CCTCTTAACC TGCAGGTCGT AGGCTTGACT 
ACCTCTACCA CCCGACTGAC CTGGGAGCCT CCAGCGTTGT CTGAACGTAA CGGACGGATC 
ATACATTACA TTGTGGTTTA CCGAGACATC AACAGCAAAC AAAACAGCAC CAACCGAACC 
TCCGACACAC AGATGACCAT CCAAGGGCTC CGACCTGACA CCACCTATGA TATCCGTGTG 
CAGGCGTTCA CTAGCAAGGG TGGAGGTCCC ATAAGTCCCA GTATCCAGAG CAGGACCATG 
TCTACGTCAC CTGCATTTGC CACAAGTTTT GGTGTCAAAG CTGTGATGAA AACCTCTGTC 
CTTCTCACCT GGGAGGTGCC AGAAAATTAT AAATCACAAG TGCCTTTTAA GATCCTCTAT 
AACCAGCAGA GTGTGGAAGT CCAGGGGAAT CTGAAGAGGA AGCTGATCAC ACGCCTGCAG 
CCGGACACTG ATTACTCGTT TGTGCTGATG AGCCGGGGAA ACAGCGCCGG TGGCCTACAA 
CAGCAGGTCT CCATCCGCAC TGCACCAGAC CTGTTCAAAA CTAAACCTGT CCTGTACACG 
GCAGACCAGA CCAACAATGG CAAACTCACC ATCAACCTGC CCAAAGTGCC CACCTCTGCT 
CCTGTCAGGT GTTATTACAT TGTGGTCGTG CCCGTCTCTC AGACATCATC TCGTCAGTGG 
ATTAATCCCG ATGAGATGGA GCTGGATGAG CTCCTGGAGT CTAGTGAGGG AGCTCATCTC 
AGGAGACGTC GACGGCACAC AGACACCGTA AGACCGTACA TCGCCGCAAA GCTATCCACG 
CTCCCAGAAA CCTTCACTCT GGGCGATGAG CTAGACTACA GCGGCTTCAT CAACCGGCCT 
CTTCCCAACA ACCAACACTT CCAGTGTTTT GTGATGGCTG AACTCAAGGA CCAATACCCT 
GTTACTGCCA ATGAGAAGCA GCGGACCTTC TCGGCTAGCC CATACTCAGA CCCCATCATA 
GTGCAGGGTG AAAACCAAAT GCAGCGCAGT GTGGACCAAC CGGAGATGCT TTGGGTCATG 
GGCCCTGTGC TCGCTGTGGT GCTCATCATC ATCATAGTCA TCGCCATCCT GCTCTTCAAA 
AGCAAACAGG AGAGGAAGAG GGCTTCCCCC CTACCAAAAG ACGATCATTC GGGTGGTGTG 
AAGGATTGTC TTCTGGCCAG CTCATCCGAT CCTGTCGAAA TGAGGAGACT CAACTACCAG 
ACACCAGGAA TGAGGGAACA TCCTCCCATA TCCGTCTCCG AACTGGCAGA CCACATCGAG 
CGCCTCAAGG CCAACGATGC CCTCCGGTTT TCCCAAGAGT ATGAGTCTAT TGATCCAGGC 
CAGCAGTTCA CATGGGAGAA CTCCAACTTG GAGGTCAACA AGCCAAAGAA CCGTTATGCC 
AACGTTATTG CTTACGACCA CTCGAGAGTT GTTCTCACCC CTGTGGACGG TGTACCAGGG 
AGTGACTACA TTAATTCCAA CTACATTGAT GGCTACCGTA AGCAGAATGC CTACATTGCC 
ACACAAGGTC CTCTGCCTGA GACTCTGAGC GACTTCTGGA GGATGATTTG GGAGCAACGA 
GCCAGTACCA TCGTCATGAT GACCAGACTG GAAGAGAAGT CCAGAGTCAA GTGTGACCAA 
TACTGGCCCA TTCGTGGCAC AGAGACTTAT GGAATGATTC AAGTGACCAT GCTTGATGCT 
GTTGAGCTGG CAACCTACAG TGTGCGCACC TTTGCCCTTT ACAAGAATGG CTCTAGTGAG 
AAGCGCGAGG TCAGGCAGTT TCAGTTCATG GCTTGGCCGG ACCACGGAGT GCCTGAATAT 
CCCACCCCCA TCCTAGCCTT CCTGCGCAGG GTCAAAGCCT GCAACCCTCC TGATGCTGGA 
CCCATGGTAG TGCACTGCAG TGCTGGAGTT GGCCGTACAG GCTGCTTCAT AGTCATCGAT 
GCGATGTTGG AGCGCATGAA GCACGAGAAG ACCGTAGACA TTTATGGCCA CGTCACGTGC 
ATGAGATCCC AGAGGAACTA TATGGTGCAG ACGGAGGACC AGTACATCTT TATCCACGAG 
GCCCTTCTGG AGGCCGCCAC CTGTGGAAAC ACAGAGGTCC CTGCCCGAAA CCTCTACGCC 
CACATCCAGA AGCTCACACA GCCATCGGCT GGAGAGACCG TCACAGCCAT GGAGCTGGAG 
TTTAAGAGAC TCGCCAACTC CAAAGCCCAC ACCTCAAGAT TCATCAGCGC CAACCTGCCC 
TGCAACAAGT TCAAGAACCG GCTGGTCAAT ATCATGCCTT TCGAGTCCAC ACGGGTGTGC 
CTGCAGCCAA TCCGAGGAGT GGAGGGGTCC GACTACATCA ATGCCAGCTG CATTGACGGA 
TACAGACAGC AGAAGGCCTA TATTGCCACT CAGGGTCCTC TAGCGGAGAC CACCGAGGAC 
TTCTGGAGGA TGTTATGGGA GCACAATTCC ACTATTGTAG TGATGCTGAC CAAACTCAGA 
GAGATGGGAC GGGAGAAGTG TCATCAGTAC TGGCCCGCCG AGCGCTCAGC CCGCTATCAG 
TACTTCGTAG TGGATCCCAT GGCCGAATAC AACATGCCCC AGTACATCCT GCGAGAGTTC 
AAAGTCACAG ATGCCAGGGA CGGTCAATCC AGAACCATTC GCCAGTTCCA GTTCACTGAT 
TGGCCAGAGC AGGGAGTGCC AAAAACCGGT GAGGGGTTCA TCGATTTCAT TGGGCAAGTT 
CATAAAACCA AGGAGCAGTT TGGGCAGGAT GGACCAATCA CTGTGCACTG CAGTGCTGGA 
GTGGGAAGAA CAGGTGTTTT CATCACTCTC AGTATTGTCC TGGAGAGGAT GAGATATGAG 
GGGGTGGTGG ATCTGTTCCA GACGGTTAAA ACCCTGCGCA CACAAAGGCC TGCCATGGTG 
CAGACAGAGG ACCAGTACCA GCTGTGCTAC AGAGCCGCTC TGGAGTACCT GGGGAGCTTT 
GATCACTATG CAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296743.1
CDS	425..5902
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVIREDPRGVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298468.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298468.2

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGTGCCA 
GGAGCACCGC CTCGGAAATT GGAAGTGGAG GCTTTGAACT CCACCGCTAT CCGTGTGACA 
TGGAAACCTC CTCTTTCTGG AAAGCAGCAT GGCCAGATCC GGGGATATCA GGTCATATAC 
TCCCGGTTGG AGAACGGGGA ACCACGGGGA CATCCGAATA TAATGGATGT CGCTCTGCCA 
GAAGCACAGT GGAAAATTGA AGAGTCTACG GAATATGAGG CTGTGATCGC TGGACTTGCC 
TCTGAAACCT CTTACTCAGT GACTGTGGCT GCGTACACAA CCAAAGGTGA TGGAGCTCGC 
AGCAAAGCCA AAGTGGTCAC CACCACCGGA GCAGTGCCTG GCAAACCCAC TATGATCATC 
AGCACCACCG TTGGCAACAC GGCCCTTATC CAGTGGCAGC CTCCTAAAGA CATGGTGGGT 
GAGCTGATGG GCTACCGGCT GCGCTACAAA CGTCTGGAAG AGGAAAACTA TGAGATACGC 
GAATTCAGTC GCACCGACGA CCACCACACC GTCACTGACC TGCACAAGGG CGCCACCTAC 
AGCTTCCACC TGAGCGCCAG AAACCGCGCT GGACTCGGAG AGGAGTACGT TAAAGACATC 
GGCACTCCTG AGGACGTGCC CAGCGGGTTT CCTCTTAACC TGCAGGTCGT AGGCTTGACT 
ACCTCTACCA CCCGACTGAC CTGGGAGCCT CCAGCGTTGT CTGAACGTAA CGGACGGATC 
ATACATTACA TTGTGGTTTA CCGAGACATC AACAGCAAAC AAAACAGCAC CAACCGAACC 
TCCGACACAC AGATGACCAT CCAAGGGCTC CGACCTGACA CCACCTATGA TATCCGTGTG 
CAGGCGTTCA CTAGCAAGGG TGGAGGTCCC ATAAGTCCCA GTATCCAGAG CAGGACCATG 
TCTACGTCAC CTGCATTTGC CACAAGTTTT GGTGTCAAAG CTGTGATGAA AACCTCTGTC 
CTTCTCACCT GGGAGGTGCC AGAAAATTAT AAATCACAAG TGCCTTTTAA GATCCTCTAT 
AACCAGCAGA GTGTGGAAGT CCAGGGGAAT CTGAAGAGGA AGCTGATCAC ACGCCTGCAG 
CCGGACACTG ATTACTCGTT TGTGCTGATG AGCCGGGGAA ACAGCGCCGG TGGCCTACAA 
CAGCAGGTCT CCATCCGCAC TGCACCAGAC CTGTTCAAAA CTAAACCTGT CCTGTACACG 
GCAGACCAGA CCAACAATGG CAAACTCACC ATCAACCTGC CCAAAGTGCC CACCTCTGCT 
CCTGTCAGGT GTTATTACAT TGTGGTCGTG CCCGTCTCTC AGACATCATC TCGTCAGTGG 
ATTAATCCCG ATGAGATGGA GCTGGATGAG CTCCTGGAGT CTAGTGAGGG AGCTCATCTC 
AGGAGACGTC GACGGCACAC AGACACCGTA AGACCGTACA TCGCCGCAAA GCTATCCACG 
CTCCCAGAAA CCTTCACTCT GGGCGATGAG CTAGACTACA GCGGCTTCAT CAACCGGCCT 
CTTCCCAACA ACCAACACTT CCAGTGTTTT GTGATGGCTG AACTCAAGGA CCAATACCCT 
GTTACTGCCA ATGAGAAGCA GCGGACCTTC TCGGCTAGCC CATACTCAGA CCCCATCATA 
GTGCAGGGTG AAAACCAAAT GCAGCGCAGT GTGGACCAAC CGGAGATGCT TTGGGTCATG 
GGCCCTGTGC TCGCTGTGGT GCTCATCATC ATCATAGTCA TCGCCATCCT GCTCTTCAAA 
AGCAAACAGG AGAGGAAGAG GGCTTCCCCC CTACCAAAAG ACGATCATTC GGGTGGTGTG 
AAGGATTGTC TTCTGGCCAG CTCATCCGAT CCTGTCGAAA TGAGGAGACT CAACTACCAG 
ACACCAGGAA TGAGGGAACA TCCTCCCATA TCCGTCTCCG AACTGGCAGA CCACATCGAG 
CGCCTCAAGG CCAACGATGC CCTCCGGTTT TCCCAAGAGT ATGAGTCTAT TGATCCAGGC 
CAGCAGTTCA CATGGGAGAA CTCCAACTTG GAGGTCAACA AGCCAAAGAA CCGTTATGCC 
AACGTTATTG CTTACGACCA CTCGAGAGTT GTTCTCACCC CTGTGGACGG TGTACCAGGG 
AGTGACTACA TTAATTCCAA CTACATTGAT GGCTACCGTA AGCAGAATGC CTACATTGCC 
ACACAAGGTC CTCTGCCTGA GACTCTGAGC GACTTCTGGA GGATGATTTG GGAGCAACGA 
GCCAGTACCA TCGTCATGAT GACCAGACTG GAAGAGAAGT CCAGAGTCAA GTGTGACCAA 
TACTGGCCCA TTCGTGGCAC AGAGACTTAT GGAATGATTC AAGTGACCAT GCTTGATGCT 
GTTGAGCTGG CAACCTACAG TGTGCGCACC TTTGCCCTTT ACAAGAATGG CTCTAGTGAG 
AAGCGCGAGG TCAGGCAGTT TCAGTTCATG GCTTGGCCGG ACCACGGAGT GCCTGAATAT 
CCCACCCCCA TCCTAGCCTT CCTGCGCAGG GTCAAAGCCT GCAACCCTCC TGATGCTGGA 
CCCATGGTAG TGCACTGCAG TGCTGGAGTT GGCCGTACAG GCTGCTTCAT AGTCATCGAT 
GCGATGTTGG AGCGCATGAA GCACGAGAAG ACCGTAGACA TTTATGGCCA CGTCACGTGC 
ATGAGATCCC AGAGGAACTA TATGGTGCAG ACGGAGGACC AGTACATCTT TATCCACGAG 
GCCCTTCTGG AGGCCGCCAC CTGTGGAAAC ACAGAGGTCC CTGCCCGAAA CCTCTACGCC 
CACATCCAGA AGCTCACACA GCCATCGGCT GGAGAGACCG TCACAGCCAT GGAGCTGGAG 
TTTAAGAGAC TCGCCAACTC CAAAGCCCAC ACCTCAAGAT TCATCAGCGC CAACCTGCCC 
TGCAACAAGT TCAAGAACCG GCTGGTCAAT ATCATGCCTT TCGAGTCCAC ACGGGTGTGC 
CTGCAGCCAA TCCGAGGAGT GGAGGGGTCC GACTACATCA ATGCCAGCTG CATTGACGGA 
TACAGACAGC AGAAGGCCTA TATTGCCACT CAGGGTCCTC TAGCGGAGAC CACCGAGGAC 
TTCTGGAGGA TGTTATGGGA GCACAATTCC ACTATTGTAG TGATGCTGAC CAAACTCAGA 
GAGATGGGAC GGGAGAAGTG TCATCAGTAC TGGCCCGCCG AGCGCTCAGC CCGCTATCAG 
TACTTCGTAG TGGATCCCAT GGCCGAATAC AACATGCCCC AGTACATCCT GCGAGAGTTC 
AAAGTCACAG ATGCCAGGGA CGGTCAATCC AGAACCATTC GCCAGTTCCA GTTCACTGAT 
TGGCCAGAGC AGGGAGTGCC AAAAACCGGT GAGGGGTTCA TCGATTTCAT TGGGCAAGTT 
CATAAAACCA AGGAGCAGTT TGGGCAGGAT GGACCAATCA CTGTGCACTG CAGTGCTGGA 
GTGGGAAGAA CAGGTGTTTT CATCACTCTC AGTATTGTCC TGGAGAGGAT GAGATATGAG 
GGGGTGGTGG ATCTGTTCCA GACGGTTAAA ACCCTGCGCA CACAAAGGCC TGCCATGGTG 
CAGACAGAGG ACCAGTACCA GCTGTGCTAC AGAGCCGCTC TGGAGTACCT GGGGAGCTTT 
GATCACTATG CAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298468.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296743.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X13, mRNA.

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CloneID	ODa47422
Clone ID Related Accession (Same CDS sequence)	XM_009298468.2 , XM_009298468.3
Accession Version	XM_009298468.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298468.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGTGCCA 
GGAGCACCGC CTCGGAAATT GGAAGTGGAG GCTTTGAACT CCACCGCTAT CCGTGTGACA 
TGGAAACCTC CTCTTTCTGG AAAGCAGCAT GGCCAGATCC GGGGATATCA GGTCATATAC 
TCCCGGTTGG AGAACGGGGA ACCACGGGGA CATCCGAATA TAATGGATGT CGCTCTGCCA 
GAAGCACAGT GGAAAATTGA AGAGTCTACG GAATATGAGG CTGTGATCGC TGGACTTGCC 
TCTGAAACCT CTTACTCAGT GACTGTGGCT GCGTACACAA CCAAAGGTGA TGGAGCTCGC 
AGCAAAGCCA AAGTGGTCAC CACCACCGGA GCAGTGCCTG GCAAACCCAC TATGATCATC 
AGCACCACCG TTGGCAACAC GGCCCTTATC CAGTGGCAGC CTCCTAAAGA CATGGTGGGT 
GAGCTGATGG GCTACCGGCT GCGCTACAAA CGTCTGGAAG AGGAAAACTA TGAGATACGC 
GAATTCAGTC GCACCGACGA CCACCACACC GTCACTGACC TGCACAAGGG CGCCACCTAC 
AGCTTCCACC TGAGCGCCAG AAACCGCGCT GGACTCGGAG AGGAGTACGT TAAAGACATC 
GGCACTCCTG AGGACGTGCC CAGCGGGTTT CCTCTTAACC TGCAGGTCGT AGGCTTGACT 
ACCTCTACCA CCCGACTGAC CTGGGAGCCT CCAGCGTTGT CTGAACGTAA CGGACGGATC 
ATACATTACA TTGTGGTTTA CCGAGACATC AACAGCAAAC AAAACAGCAC CAACCGAACC 
TCCGACACAC AGATGACCAT CCAAGGGCTC CGACCTGACA CCACCTATGA TATCCGTGTG 
CAGGCGTTCA CTAGCAAGGG TGGAGGTCCC ATAAGTCCCA GTATCCAGAG CAGGACCATG 
TCTACGTCAC CTGCATTTGC CACAAGTTTT GGTGTCAAAG CTGTGATGAA AACCTCTGTC 
CTTCTCACCT GGGAGGTGCC AGAAAATTAT AAATCACAAG TGCCTTTTAA GATCCTCTAT 
AACCAGCAGA GTGTGGAAGT CCAGGGGAAT CTGAAGAGGA AGCTGATCAC ACGCCTGCAG 
CCGGACACTG ATTACTCGTT TGTGCTGATG AGCCGGGGAA ACAGCGCCGG TGGCCTACAA 
CAGCAGGTCT CCATCCGCAC TGCACCAGAC CTGTTCAAAA CTAAACCTGT CCTGTACACG 
GCAGACCAGA CCAACAATGG CAAACTCACC ATCAACCTGC CCAAAGTGCC CACCTCTGCT 
CCTGTCAGGT GTTATTACAT TGTGGTCGTG CCCGTCTCTC AGACATCATC TCGTCAGTGG 
ATTAATCCCG ATGAGATGGA GCTGGATGAG CTCCTGGAGT CTAGTGAGGG AGCTCATCTC 
AGGAGACGTC GACGGCACAC AGACACCGTA AGACCGTACA TCGCCGCAAA GCTATCCACG 
CTCCCAGAAA CCTTCACTCT GGGCGATGAG CTAGACTACA GCGGCTTCAT CAACCGGCCT 
CTTCCCAACA ACCAACACTT CCAGTGTTTT GTGATGGCTG AACTCAAGGA CCAATACCCT 
GTTACTGCCA ATGAGAAGCA GCGGACCTTC TCGGCTAGCC CATACTCAGA CCCCATCATA 
GTGCAGGGTG AAAACCAAAT GCAGCGCAGT GTGGACCAAC CGGAGATGCT TTGGGTCATG 
GGCCCTGTGC TCGCTGTGGT GCTCATCATC ATCATAGTCA TCGCCATCCT GCTCTTCAAA 
AGCAAACAGG AGAGGAAGAG GGCTTCCCCC CTACCAAAAG ACGATCATTC GGGTGGTGTG 
AAGGATTGTC TTCTGGCCAG CTCATCCGAT CCTGTCGAAA TGAGGAGACT CAACTACCAG 
ACACCAGGAA TGAGGGAACA TCCTCCCATA TCCGTCTCCG AACTGGCAGA CCACATCGAG 
CGCCTCAAGG CCAACGATGC CCTCCGGTTT TCCCAAGAGT ATGAGTCTAT TGATCCAGGC 
CAGCAGTTCA CATGGGAGAA CTCCAACTTG GAGGTCAACA AGCCAAAGAA CCGTTATGCC 
AACGTTATTG CTTACGACCA CTCGAGAGTT GTTCTCACCC CTGTGGACGG TGTACCAGGG 
AGTGACTACA TTAATTCCAA CTACATTGAT GGCTACCGTA AGCAGAATGC CTACATTGCC 
ACACAAGGTC CTCTGCCTGA GACTCTGAGC GACTTCTGGA GGATGATTTG GGAGCAACGA 
GCCAGTACCA TCGTCATGAT GACCAGACTG GAAGAGAAGT CCAGAGTCAA GTGTGACCAA 
TACTGGCCCA TTCGTGGCAC AGAGACTTAT GGAATGATTC AAGTGACCAT GCTTGATGCT 
GTTGAGCTGG CAACCTACAG TGTGCGCACC TTTGCCCTTT ACAAGAATGG CTCTAGTGAG 
AAGCGCGAGG TCAGGCAGTT TCAGTTCATG GCTTGGCCGG ACCACGGAGT GCCTGAATAT 
CCCACCCCCA TCCTAGCCTT CCTGCGCAGG GTCAAAGCCT GCAACCCTCC TGATGCTGGA 
CCCATGGTAG TGCACTGCAG TGCTGGAGTT GGCCGTACAG GCTGCTTCAT AGTCATCGAT 
GCGATGTTGG AGCGCATGAA GCACGAGAAG ACCGTAGACA TTTATGGCCA CGTCACGTGC 
ATGAGATCCC AGAGGAACTA TATGGTGCAG ACGGAGGACC AGTACATCTT TATCCACGAG 
GCCCTTCTGG AGGCCGCCAC CTGTGGAAAC ACAGAGGTCC CTGCCCGAAA CCTCTACGCC 
CACATCCAGA AGCTCACACA GCCATCGGCT GGAGAGACCG TCACAGCCAT GGAGCTGGAG 
TTTAAGAGAC TCGCCAACTC CAAAGCCCAC ACCTCAAGAT TCATCAGCGC CAACCTGCCC 
TGCAACAAGT TCAAGAACCG GCTGGTCAAT ATCATGCCTT TCGAGTCCAC ACGGGTGTGC 
CTGCAGCCAA TCCGAGGAGT GGAGGGGTCC GACTACATCA ATGCCAGCTG CATTGACGGA 
TACAGACAGC AGAAGGCCTA TATTGCCACT CAGGGTCCTC TAGCGGAGAC CACCGAGGAC 
TTCTGGAGGA TGTTATGGGA GCACAATTCC ACTATTGTAG TGATGCTGAC CAAACTCAGA 
GAGATGGGAC GGGAGAAGTG TCATCAGTAC TGGCCCGCCG AGCGCTCAGC CCGCTATCAG 
TACTTCGTAG TGGATCCCAT GGCCGAATAC AACATGCCCC AGTACATCCT GCGAGAGTTC 
AAAGTCACAG ATGCCAGGGA CGGTCAATCC AGAACCATTC GCCAGTTCCA GTTCACTGAT 
TGGCCAGAGC AGGGAGTGCC AAAAACCGGT GAGGGGTTCA TCGATTTCAT TGGGCAAGTT 
CATAAAACCA AGGAGCAGTT TGGGCAGGAT GGACCAATCA CTGTGCACTG CAGTGCTGGA 
GTGGGAAGAA CAGGTGTTTT CATCACTCTC AGTATTGTCC TGGAGAGGAT GAGATATGAG 
GGGGTGGTGG ATCTGTTCCA GACGGTTAAA ACCCTGCGCA CACAAAGGCC TGCCATGGTG 
CAGACAGAGG ACCAGTACCA GCTGTGCTAC AGAGCCGCTC TGGAGTACCT GGGGAGCTTT 
GATCACTATG CAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296743.1
CDS	421..5898
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGGSPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVIREDPRGVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQWKIEESTEYEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298468.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298468.3

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGGGGTTCGC CGATACGAGG AGCTCTGCAG 
ATTGAAAACA GCGAGGAATC AGACCAGGGC AAGTACGAAT GTGTGGCTAC GAATAGCGCC 
GGGACGCGTT ATTCAGCTCC GGCTAATCTC TACGTGAGAG TCATTCGAGA AGACCCTCGA 
GGAGTACGAC GAGTTCCACC CCGTTTCTCC ATCCCACCTA CCAATCACGA GGTGATGCCA 
GGGGGCAGTG TAAACCTCAC CTGCGTGGCA GTAGGTGCCC CGATGCCCTA CGTCAAATGG 
ATGACTGGTG AGGTGGAGCT CACCAAGGAT GAGGAGATGC CTGTGGGACG CAACGTCTTG 
GAGCTCACCA ACATCCGTCA GTCGACCAAC TACACCTGCG TGGCTATATC ATCCCTAGGT 
ATGATCGAGG CAACGGCACA AGTGTCTGTC AAAGCTCTCC CAAAGCCTCC TACATCCCTG 
ACTGTTACGG AAACCACAGC TACGAGTGTT ACACTCACAT GGGACTCTGG AAACCCTGAA 
CCTGTGTCTT ACTACATAAT TCAATACCGG GCCAAGACTT CAGACACCAA CTTCCAAGAA 
GTGGACGGTG TGGCCACCAC ACGTTACAGC ATCGGCGGTC TCAGCCCATA TTCAGAGTAT 
GAGTTTCGAG TAATGGCAGT CAACAACATT GGTCGTGGGC CACCTAGTGA ACCCGTTGAA 
ACACGAACAG GTGAACAGGC ACCTTCCTCC CCACCGCTGC ATGTTCAAGC ACGAATGCTG 
AGTGCCAGCA CCATGTTGGT TCAGTGGGAA CCACCAGAGG AACCCAATGG TCAGATCAGA 
GGCTATCGGA TCTACTACAC ATCTGACCTG GATGCACCTC TTAGCGCCTG GCAGAAACAC 
AACACAGACG ATAGCCGTCT CACCACTATC TCCAGCCTGA CCACCGGCAT CACCTACAGC 
CTACGTGTGC TGGGCTTCAC ATCTGTAGGG GATGGACCAC CATCTGATGT GCTACAGGTC 
AAGACACAGC AAGGAGTTCC TGCTCAGCCA TCCAGCTTCG AAGCTGAAGC AGAGCTAGAT 
ACAAGGATCC TACTAACTTG GCTGTGGCCC GTGCAGGACC CCATCATCAA ATACGAGCTG 
CAATATTGGG AGGCTGACTC AGACAACAAG ATCCATGTCA CCTTTGACCC AGCTGGCTCG 
TACGCTGTTG AAGGTCTGAA ACCAGACACT TTGTACAAAT TCTCTCTGGC AGCCCGCTCT 
GAAATGGGAC AAGGAGTCTT CACTCACCCC ATTGAAGCCA GGACGGCCCA GTCTGTGCCA 
GGAGCACCGC CTCGGAAATT GGAAGTGGAG GCTTTGAACT CCACCGCTAT CCGTGTGACA 
TGGAAACCTC CTCTTTCTGG AAAGCAGCAT GGCCAGATCC GGGGATATCA GGTCATATAC 
TCCCGGTTGG AGAACGGGGA ACCACGGGGA CATCCGAATA TAATGGATGT CGCTCTGCCA 
GAAGCACAGT GGAAAATTGA AGAGTCTACG GAATATGAGG CTGTGATCGC TGGACTTGCC 
TCTGAAACCT CTTACTCAGT GACTGTGGCT GCGTACACAA CCAAAGGTGA TGGAGCTCGC 
AGCAAAGCCA AAGTGGTCAC CACCACCGGA GCAGTGCCTG GCAAACCCAC TATGATCATC 
AGCACCACCG TTGGCAACAC GGCCCTTATC CAGTGGCAGC CTCCTAAAGA CATGGTGGGT 
GAGCTGATGG GCTACCGGCT GCGCTACAAA CGTCTGGAAG AGGAAAACTA TGAGATACGC 
GAATTCAGTC GCACCGACGA CCACCACACC GTCACTGACC TGCACAAGGG CGCCACCTAC 
AGCTTCCACC TGAGCGCCAG AAACCGCGCT GGACTCGGAG AGGAGTACGT TAAAGACATC 
GGCACTCCTG AGGACGTGCC CAGCGGGTTT CCTCTTAACC TGCAGGTCGT AGGCTTGACT 
ACCTCTACCA CCCGACTGAC CTGGGAGCCT CCAGCGTTGT CTGAACGTAA CGGACGGATC 
ATACATTACA TTGTGGTTTA CCGAGACATC AACAGCAAAC AAAACAGCAC CAACCGAACC 
TCCGACACAC AGATGACCAT CCAAGGGCTC CGACCTGACA CCACCTATGA TATCCGTGTG 
CAGGCGTTCA CTAGCAAGGG TGGAGGTCCC ATAAGTCCCA GTATCCAGAG CAGGACCATG 
TCTACGTCAC CTGCATTTGC CACAAGTTTT GGTGTCAAAG CTGTGATGAA AACCTCTGTC 
CTTCTCACCT GGGAGGTGCC AGAAAATTAT AAATCACAAG TGCCTTTTAA GATCCTCTAT 
AACCAGCAGA GTGTGGAAGT CCAGGGGAAT CTGAAGAGGA AGCTGATCAC ACGCCTGCAG 
CCGGACACTG ATTACTCGTT TGTGCTGATG AGCCGGGGAA ACAGCGCCGG TGGCCTACAA 
CAGCAGGTCT CCATCCGCAC TGCACCAGAC CTGTTCAAAA CTAAACCTGT CCTGTACACG 
GCAGACCAGA CCAACAATGG CAAACTCACC ATCAACCTGC CCAAAGTGCC CACCTCTGCT 
CCTGTCAGGT GTTATTACAT TGTGGTCGTG CCCGTCTCTC AGACATCATC TCGTCAGTGG 
ATTAATCCCG ATGAGATGGA GCTGGATGAG CTCCTGGAGT CTAGTGAGGG AGCTCATCTC 
AGGAGACGTC GACGGCACAC AGACACCGTA AGACCGTACA TCGCCGCAAA GCTATCCACG 
CTCCCAGAAA CCTTCACTCT GGGCGATGAG CTAGACTACA GCGGCTTCAT CAACCGGCCT 
CTTCCCAACA ACCAACACTT CCAGTGTTTT GTGATGGCTG AACTCAAGGA CCAATACCCT 
GTTACTGCCA ATGAGAAGCA GCGGACCTTC TCGGCTAGCC CATACTCAGA CCCCATCATA 
GTGCAGGGTG AAAACCAAAT GCAGCGCAGT GTGGACCAAC CGGAGATGCT TTGGGTCATG 
GGCCCTGTGC TCGCTGTGGT GCTCATCATC ATCATAGTCA TCGCCATCCT GCTCTTCAAA 
AGCAAACAGG AGAGGAAGAG GGCTTCCCCC CTACCAAAAG ACGATCATTC GGGTGGTGTG 
AAGGATTGTC TTCTGGCCAG CTCATCCGAT CCTGTCGAAA TGAGGAGACT CAACTACCAG 
ACACCAGGAA TGAGGGAACA TCCTCCCATA TCCGTCTCCG AACTGGCAGA CCACATCGAG 
CGCCTCAAGG CCAACGATGC CCTCCGGTTT TCCCAAGAGT ATGAGTCTAT TGATCCAGGC 
CAGCAGTTCA CATGGGAGAA CTCCAACTTG GAGGTCAACA AGCCAAAGAA CCGTTATGCC 
AACGTTATTG CTTACGACCA CTCGAGAGTT GTTCTCACCC CTGTGGACGG TGTACCAGGG 
AGTGACTACA TTAATTCCAA CTACATTGAT GGCTACCGTA AGCAGAATGC CTACATTGCC 
ACACAAGGTC CTCTGCCTGA GACTCTGAGC GACTTCTGGA GGATGATTTG GGAGCAACGA 
GCCAGTACCA TCGTCATGAT GACCAGACTG GAAGAGAAGT CCAGAGTCAA GTGTGACCAA 
TACTGGCCCA TTCGTGGCAC AGAGACTTAT GGAATGATTC AAGTGACCAT GCTTGATGCT 
GTTGAGCTGG CAACCTACAG TGTGCGCACC TTTGCCCTTT ACAAGAATGG CTCTAGTGAG 
AAGCGCGAGG TCAGGCAGTT TCAGTTCATG GCTTGGCCGG ACCACGGAGT GCCTGAATAT 
CCCACCCCCA TCCTAGCCTT CCTGCGCAGG GTCAAAGCCT GCAACCCTCC TGATGCTGGA 
CCCATGGTAG TGCACTGCAG TGCTGGAGTT GGCCGTACAG GCTGCTTCAT AGTCATCGAT 
GCGATGTTGG AGCGCATGAA GCACGAGAAG ACCGTAGACA TTTATGGCCA CGTCACGTGC 
ATGAGATCCC AGAGGAACTA TATGGTGCAG ACGGAGGACC AGTACATCTT TATCCACGAG 
GCCCTTCTGG AGGCCGCCAC CTGTGGAAAC ACAGAGGTCC CTGCCCGAAA CCTCTACGCC 
CACATCCAGA AGCTCACACA GCCATCGGCT GGAGAGACCG TCACAGCCAT GGAGCTGGAG 
TTTAAGAGAC TCGCCAACTC CAAAGCCCAC ACCTCAAGAT TCATCAGCGC CAACCTGCCC 
TGCAACAAGT TCAAGAACCG GCTGGTCAAT ATCATGCCTT TCGAGTCCAC ACGGGTGTGC 
CTGCAGCCAA TCCGAGGAGT GGAGGGGTCC GACTACATCA ATGCCAGCTG CATTGACGGA 
TACAGACAGC AGAAGGCCTA TATTGCCACT CAGGGTCCTC TAGCGGAGAC CACCGAGGAC 
TTCTGGAGGA TGTTATGGGA GCACAATTCC ACTATTGTAG TGATGCTGAC CAAACTCAGA 
GAGATGGGAC GGGAGAAGTG TCATCAGTAC TGGCCCGCCG AGCGCTCAGC CCGCTATCAG 
TACTTCGTAG TGGATCCCAT GGCCGAATAC AACATGCCCC AGTACATCCT GCGAGAGTTC 
AAAGTCACAG ATGCCAGGGA CGGTCAATCC AGAACCATTC GCCAGTTCCA GTTCACTGAT 
TGGCCAGAGC AGGGAGTGCC AAAAACCGGT GAGGGGTTCA TCGATTTCAT TGGGCAAGTT 
CATAAAACCA AGGAGCAGTT TGGGCAGGAT GGACCAATCA CTGTGCACTG CAGTGCTGGA 
GTGGGAAGAA CAGGTGTTTT CATCACTCTC AGTATTGTCC TGGAGAGGAT GAGATATGAG 
GGGGTGGTGG ATCTGTTCCA GACGGTTAAA ACCCTGCGCA CACAAAGGCC TGCCATGGTG 
CAGACAGAGG ACCAGTACCA GCTGTGCTAC AGAGCCGCTC TGGAGTACCT GGGGAGCTTT 
GATCACTATG CAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298469.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296744.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X12, mRNA.

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CloneID	ODa47423
Clone ID Related Accession (Same CDS sequence)	XM_009298469.2 , XM_009298469.3
Accession Version	XM_009298469.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298469.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGTGCCAGG AGCACCGCCT CGGAAATTGG AAGTGGAGGC TTTGAACTCC 
ACCGCTATCC GTGTGACATG GAAACCTCCT CTTTCTGGAA AGCAGCATGG CCAGATCCGG 
GGATATCAGG TCATATACTC CCGGTTGGAG AACGGGGAAC CACGGGGACA TCCGAATATA 
ATGGATGTCG CTCTGCCAGA AGCACAGGAG GCTGTGATCG CTGGACTTGC CTCTGAAACC 
TCTTACTCAG TGACTGTGGC TGCGTACACA ACCAAAGGTG ATGGAGCTCG CAGCAAAGCC 
AAAGTGGTCA CCACCACCGG AGCAGTGCCT GGCAAACCCA CTATGATCAT CAGCACCACC 
GTTGGCAACA CGGCCCTTAT CCAGTGGCAG CCTCCTAAAG ACATGGTGGG TGAGCTGATG 
GGCTACCGGC TGCGCTACAA ACGTCTGGAA GAGGAAAACT ATGAGATACG CGAATTCAGT 
CGCACCGACG ACCACCACAC CGTCACTGAC CTGCACAAGG GCGCCACCTA CAGCTTCCAC 
CTGAGCGCCA GAAACCGCGC TGGACTCGGA GAGGAGTACG TTAAAGACAT CGGCACTCCT 
GAGGACGTGC CCAGCGGGTT TCCTCTTAAC CTGCAGGTCG TAGGCTTGAC TACCTCTACC 
ACCCGACTGA CCTGGGAGCC TCCAGCGTTG TCTGAACGTA ACGGACGGAT CATACATTAC 
ATTGTGGTTT ACCGAGACAT CAACAGCAAA CAAAACAGCA CCAACCGAAC CTCCGACACA 
CAGATGACCA TCCAAGGGCT CCGACCTGAC ACCACCTATG ATATCCGTGT GCAGGCGTTC 
ACTAGCAAGG GTGGAGGTCC CATAAGTCCC AGTATCCAGA GCAGGACCAT GTCTACGTCA 
CCTGCATTTG CCACAAGTTT TGGTGTCAAA GCTGTGATGA AAACCTCTGT CCTTCTCACC 
TGGGAGGTGC CAGAAAATTA TAAATCACAA GTGCCTTTTA AGATCCTCTA TAACCAGCAG 
AGTGTGGAAG TCCAGGGGAA TCTGAAGAGG AAGCTGATCA CACGCCTGCA GCCGGACACT 
GATTACTCGT TTGTGCTGAT GAGCCGGGGA AACAGCGCCG GTGGCCTACA ACAGCAGGTC 
TCCATCCGCA CTGCACCAGA CCTGTTCAAA ACTAAACCTG TCCTGTACAC GGCAGACCAG 
ACCAACAATG GCAAACTCAC CATCAACCTG CCCAAAGTGC CCACCTCTGC TCCTGTCAGG 
TGTTATTACA TTGTGGTCGT GCCCGTCTCT CAGACATCAT CTCGTCAGTG GATTAATCCC 
GATGAGATGG AGCTGGATGA GCTCCTGGAG TCTAGTGAGG GAGCTCATCT CAGGAGACGT 
CGACGGCACA CAGACACCGT AAGACCGTAC ATCGCCGCAA AGCTATCCAC GCTCCCAGAA 
ACCTTCACTC TGGGCGATGA GCTAGACTAC AGCGGCTTCA TCAACCGGCC TCTTCCCAAC 
AACCAACACT TCCAGTGTTT TGTGATGGCT GAACTCAAGG ACCAATACCC TGTTACTGCC 
AATGAGAAGC AGCGGACCTT CTCGGCTAGC CCATACTCAG ACCCCATCAT AGTGCAGGGT 
GAAAACCAAA TGCAGCGCAG TGTGGACCAA CCGGAGATGC TTTGGGTCAT GGGCCCTGTG 
CTCGCTGTGG TGCTCATCAT CATCATAGTC ATCGCCATCC TGCTCTTCAA AAGCAAACAG 
GAGAGGAAGA GGGCTTCCCC CCTACCAAAA GACGATCATT CGGGTGGTGT GAAGGATTGT 
CTTCTGGCCA GCTCATCCGA TCCTGTCGAA ATGAGGAGAC TCAACTACCA GACACCAGGA 
ATGAGGGAAC ATCCTCCCAT ATCCGTCTCC GAACTGGCAG ACCACATCGA GCGCCTCAAG 
GCCAACGATG CCCTCCGGTT TTCCCAAGAG TATGAGTCTA TTGATCCAGG CCAGCAGTTC 
ACATGGGAGA ACTCCAACTT GGAGGTCAAC AAGCCAAAGA ACCGTTATGC CAACGTTATT 
GCTTACGACC ACTCGAGAGT TGTTCTCACC CCTGTGGACG GTGTACCAGG GAGTGACTAC 
ATTAATTCCA ACTACATTGA TGGCTACCGT AAGCAGAATG CCTACATTGC CACACAAGGT 
CCTCTGCCTG AGACTCTGAG CGACTTCTGG AGGATGATTT GGGAGCAACG AGCCAGTACC 
ATCGTCATGA TGACCAGACT GGAAGAGAAG TCCAGAGTCA AGTGTGACCA ATACTGGCCC 
ATTCGTGGCA CAGAGACTTA TGGAATGATT CAAGTGACCA TGCTTGATGC TGTTGAGCTG 
GCAACCTACA GTGTGCGCAC CTTTGCCCTT TACAAGAATG GCTCTAGTGA GAAGCGCGAG 
GTCAGGCAGT TTCAGTTCAT GGCTTGGCCG GACCACGGAG TGCCTGAATA TCCCACCCCC 
ATCCTAGCCT TCCTGCGCAG GGTCAAAGCC TGCAACCCTC CTGATGCTGG ACCCATGGTA 
GTGCACTGCA GTGCTGGAGT TGGCCGTACA GGCTGCTTCA TAGTCATCGA TGCGATGTTG 
GAGCGCATGA AGCACGAGAA GACCGTAGAC ATTTATGGCC ACGTCACGTG CATGAGATCC 
CAGAGGAACT ATATGGTGCA GACGGAGGAC CAGTACATCT TTATCCACGA GGCCCTTCTG 
GAGGCCGCCA CCTGTGGAAA CACAGAGGTC CCTGCCCGAA ACCTCTACGC CCACATCCAG 
AAGCTCACAC AGCCATCGGC TGGAGAGACC GTCACAGCCA TGGAGCTGGA GTTTAAGAGA 
CTCGCCAACT CCAAAGCCCA CACCTCAAGA TTCATCAGCG CCAACCTGCC CTGCAACAAG 
TTCAAGAACC GGCTGGTCAA TATCATGCCT TTCGAGTCCA CACGGGTGTG CCTGCAGCCA 
ATCCGAGGAG TGGAGGGGTC CGACTACATC AATGCCAGCT GCATTGACGG ATACAGACAG 
CAGAAGGCCT ATATTGCCAC TCAGGGTCCT CTAGCGGAGA CCACCGAGGA CTTCTGGAGG 
ATGTTATGGG AGCACAATTC CACTATTGTA GTGATGCTGA CCAAACTCAG AGAGATGGGA 
CGGGAGAAGT GTCATCAGTA CTGGCCCGCC GAGCGCTCAG CCCGCTATCA GTACTTCGTA 
GTGGATCCCA TGGCCGAATA CAACATGCCC CAGTACATCC TGCGAGAGTT CAAAGTCACA 
GATGCCAGGG ACGGTCAATC CAGAACCATT CGCCAGTTCC AGTTCACTGA TTGGCCAGAG 
CAGGGAGTGC CAAAAACCGG TGAGGGGTTC ATCGATTTCA TTGGGCAAGT TCATAAAACC 
AAGGAGCAGT TTGGGCAGGA TGGACCAATC ACTGTGCACT GCAGTGCTGG AGTGGGAAGA 
ACAGGTGTTT TCATCACTCT CAGTATTGTC CTGGAGAGGA TGAGATATGA GGGGGTGGTG 
GATCTGTTCC AGACGGTTAA AACCCTGCGC ACACAAAGGC CTGCCATGGT GCAGACAGAG 
GACCAGTACC AGCTGTGCTA CAGAGCCGCT CTGGAGTACC TGGGGAGCTT TGATCACTAT 
GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296744.1
CDS	425..5833
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298469.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298469.2

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGTGCCAGG AGCACCGCCT CGGAAATTGG AAGTGGAGGC TTTGAACTCC 
ACCGCTATCC GTGTGACATG GAAACCTCCT CTTTCTGGAA AGCAGCATGG CCAGATCCGG 
GGATATCAGG TCATATACTC CCGGTTGGAG AACGGGGAAC CACGGGGACA TCCGAATATA 
ATGGATGTCG CTCTGCCAGA AGCACAGGAG GCTGTGATCG CTGGACTTGC CTCTGAAACC 
TCTTACTCAG TGACTGTGGC TGCGTACACA ACCAAAGGTG ATGGAGCTCG CAGCAAAGCC 
AAAGTGGTCA CCACCACCGG AGCAGTGCCT GGCAAACCCA CTATGATCAT CAGCACCACC 
GTTGGCAACA CGGCCCTTAT CCAGTGGCAG CCTCCTAAAG ACATGGTGGG TGAGCTGATG 
GGCTACCGGC TGCGCTACAA ACGTCTGGAA GAGGAAAACT ATGAGATACG CGAATTCAGT 
CGCACCGACG ACCACCACAC CGTCACTGAC CTGCACAAGG GCGCCACCTA CAGCTTCCAC 
CTGAGCGCCA GAAACCGCGC TGGACTCGGA GAGGAGTACG TTAAAGACAT CGGCACTCCT 
GAGGACGTGC CCAGCGGGTT TCCTCTTAAC CTGCAGGTCG TAGGCTTGAC TACCTCTACC 
ACCCGACTGA CCTGGGAGCC TCCAGCGTTG TCTGAACGTA ACGGACGGAT CATACATTAC 
ATTGTGGTTT ACCGAGACAT CAACAGCAAA CAAAACAGCA CCAACCGAAC CTCCGACACA 
CAGATGACCA TCCAAGGGCT CCGACCTGAC ACCACCTATG ATATCCGTGT GCAGGCGTTC 
ACTAGCAAGG GTGGAGGTCC CATAAGTCCC AGTATCCAGA GCAGGACCAT GTCTACGTCA 
CCTGCATTTG CCACAAGTTT TGGTGTCAAA GCTGTGATGA AAACCTCTGT CCTTCTCACC 
TGGGAGGTGC CAGAAAATTA TAAATCACAA GTGCCTTTTA AGATCCTCTA TAACCAGCAG 
AGTGTGGAAG TCCAGGGGAA TCTGAAGAGG AAGCTGATCA CACGCCTGCA GCCGGACACT 
GATTACTCGT TTGTGCTGAT GAGCCGGGGA AACAGCGCCG GTGGCCTACA ACAGCAGGTC 
TCCATCCGCA CTGCACCAGA CCTGTTCAAA ACTAAACCTG TCCTGTACAC GGCAGACCAG 
ACCAACAATG GCAAACTCAC CATCAACCTG CCCAAAGTGC CCACCTCTGC TCCTGTCAGG 
TGTTATTACA TTGTGGTCGT GCCCGTCTCT CAGACATCAT CTCGTCAGTG GATTAATCCC 
GATGAGATGG AGCTGGATGA GCTCCTGGAG TCTAGTGAGG GAGCTCATCT CAGGAGACGT 
CGACGGCACA CAGACACCGT AAGACCGTAC ATCGCCGCAA AGCTATCCAC GCTCCCAGAA 
ACCTTCACTC TGGGCGATGA GCTAGACTAC AGCGGCTTCA TCAACCGGCC TCTTCCCAAC 
AACCAACACT TCCAGTGTTT TGTGATGGCT GAACTCAAGG ACCAATACCC TGTTACTGCC 
AATGAGAAGC AGCGGACCTT CTCGGCTAGC CCATACTCAG ACCCCATCAT AGTGCAGGGT 
GAAAACCAAA TGCAGCGCAG TGTGGACCAA CCGGAGATGC TTTGGGTCAT GGGCCCTGTG 
CTCGCTGTGG TGCTCATCAT CATCATAGTC ATCGCCATCC TGCTCTTCAA AAGCAAACAG 
GAGAGGAAGA GGGCTTCCCC CCTACCAAAA GACGATCATT CGGGTGGTGT GAAGGATTGT 
CTTCTGGCCA GCTCATCCGA TCCTGTCGAA ATGAGGAGAC TCAACTACCA GACACCAGGA 
ATGAGGGAAC ATCCTCCCAT ATCCGTCTCC GAACTGGCAG ACCACATCGA GCGCCTCAAG 
GCCAACGATG CCCTCCGGTT TTCCCAAGAG TATGAGTCTA TTGATCCAGG CCAGCAGTTC 
ACATGGGAGA ACTCCAACTT GGAGGTCAAC AAGCCAAAGA ACCGTTATGC CAACGTTATT 
GCTTACGACC ACTCGAGAGT TGTTCTCACC CCTGTGGACG GTGTACCAGG GAGTGACTAC 
ATTAATTCCA ACTACATTGA TGGCTACCGT AAGCAGAATG CCTACATTGC CACACAAGGT 
CCTCTGCCTG AGACTCTGAG CGACTTCTGG AGGATGATTT GGGAGCAACG AGCCAGTACC 
ATCGTCATGA TGACCAGACT GGAAGAGAAG TCCAGAGTCA AGTGTGACCA ATACTGGCCC 
ATTCGTGGCA CAGAGACTTA TGGAATGATT CAAGTGACCA TGCTTGATGC TGTTGAGCTG 
GCAACCTACA GTGTGCGCAC CTTTGCCCTT TACAAGAATG GCTCTAGTGA GAAGCGCGAG 
GTCAGGCAGT TTCAGTTCAT GGCTTGGCCG GACCACGGAG TGCCTGAATA TCCCACCCCC 
ATCCTAGCCT TCCTGCGCAG GGTCAAAGCC TGCAACCCTC CTGATGCTGG ACCCATGGTA 
GTGCACTGCA GTGCTGGAGT TGGCCGTACA GGCTGCTTCA TAGTCATCGA TGCGATGTTG 
GAGCGCATGA AGCACGAGAA GACCGTAGAC ATTTATGGCC ACGTCACGTG CATGAGATCC 
CAGAGGAACT ATATGGTGCA GACGGAGGAC CAGTACATCT TTATCCACGA GGCCCTTCTG 
GAGGCCGCCA CCTGTGGAAA CACAGAGGTC CCTGCCCGAA ACCTCTACGC CCACATCCAG 
AAGCTCACAC AGCCATCGGC TGGAGAGACC GTCACAGCCA TGGAGCTGGA GTTTAAGAGA 
CTCGCCAACT CCAAAGCCCA CACCTCAAGA TTCATCAGCG CCAACCTGCC CTGCAACAAG 
TTCAAGAACC GGCTGGTCAA TATCATGCCT TTCGAGTCCA CACGGGTGTG CCTGCAGCCA 
ATCCGAGGAG TGGAGGGGTC CGACTACATC AATGCCAGCT GCATTGACGG ATACAGACAG 
CAGAAGGCCT ATATTGCCAC TCAGGGTCCT CTAGCGGAGA CCACCGAGGA CTTCTGGAGG 
ATGTTATGGG AGCACAATTC CACTATTGTA GTGATGCTGA CCAAACTCAG AGAGATGGGA 
CGGGAGAAGT GTCATCAGTA CTGGCCCGCC GAGCGCTCAG CCCGCTATCA GTACTTCGTA 
GTGGATCCCA TGGCCGAATA CAACATGCCC CAGTACATCC TGCGAGAGTT CAAAGTCACA 
GATGCCAGGG ACGGTCAATC CAGAACCATT CGCCAGTTCC AGTTCACTGA TTGGCCAGAG 
CAGGGAGTGC CAAAAACCGG TGAGGGGTTC ATCGATTTCA TTGGGCAAGT TCATAAAACC 
AAGGAGCAGT TTGGGCAGGA TGGACCAATC ACTGTGCACT GCAGTGCTGG AGTGGGAAGA 
ACAGGTGTTT TCATCACTCT CAGTATTGTC CTGGAGAGGA TGAGATATGA GGGGGTGGTG 
GATCTGTTCC AGACGGTTAA AACCCTGCGC ACACAAAGGC CTGCCATGGT GCAGACAGAG 
GACCAGTACC AGCTGTGCTA CAGAGCCGCT CTGGAGTACC TGGGGAGCTT TGATCACTAT 
GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298469.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296744.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X16, mRNA.

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CloneID	ODa47423
Clone ID Related Accession (Same CDS sequence)	XM_009298469.2 , XM_009298469.3
Accession Version	XM_009298469.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298469.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGTGCCAGG AGCACCGCCT CGGAAATTGG AAGTGGAGGC TTTGAACTCC 
ACCGCTATCC GTGTGACATG GAAACCTCCT CTTTCTGGAA AGCAGCATGG CCAGATCCGG 
GGATATCAGG TCATATACTC CCGGTTGGAG AACGGGGAAC CACGGGGACA TCCGAATATA 
ATGGATGTCG CTCTGCCAGA AGCACAGGAG GCTGTGATCG CTGGACTTGC CTCTGAAACC 
TCTTACTCAG TGACTGTGGC TGCGTACACA ACCAAAGGTG ATGGAGCTCG CAGCAAAGCC 
AAAGTGGTCA CCACCACCGG AGCAGTGCCT GGCAAACCCA CTATGATCAT CAGCACCACC 
GTTGGCAACA CGGCCCTTAT CCAGTGGCAG CCTCCTAAAG ACATGGTGGG TGAGCTGATG 
GGCTACCGGC TGCGCTACAA ACGTCTGGAA GAGGAAAACT ATGAGATACG CGAATTCAGT 
CGCACCGACG ACCACCACAC CGTCACTGAC CTGCACAAGG GCGCCACCTA CAGCTTCCAC 
CTGAGCGCCA GAAACCGCGC TGGACTCGGA GAGGAGTACG TTAAAGACAT CGGCACTCCT 
GAGGACGTGC CCAGCGGGTT TCCTCTTAAC CTGCAGGTCG TAGGCTTGAC TACCTCTACC 
ACCCGACTGA CCTGGGAGCC TCCAGCGTTG TCTGAACGTA ACGGACGGAT CATACATTAC 
ATTGTGGTTT ACCGAGACAT CAACAGCAAA CAAAACAGCA CCAACCGAAC CTCCGACACA 
CAGATGACCA TCCAAGGGCT CCGACCTGAC ACCACCTATG ATATCCGTGT GCAGGCGTTC 
ACTAGCAAGG GTGGAGGTCC CATAAGTCCC AGTATCCAGA GCAGGACCAT GTCTACGTCA 
CCTGCATTTG CCACAAGTTT TGGTGTCAAA GCTGTGATGA AAACCTCTGT CCTTCTCACC 
TGGGAGGTGC CAGAAAATTA TAAATCACAA GTGCCTTTTA AGATCCTCTA TAACCAGCAG 
AGTGTGGAAG TCCAGGGGAA TCTGAAGAGG AAGCTGATCA CACGCCTGCA GCCGGACACT 
GATTACTCGT TTGTGCTGAT GAGCCGGGGA AACAGCGCCG GTGGCCTACA ACAGCAGGTC 
TCCATCCGCA CTGCACCAGA CCTGTTCAAA ACTAAACCTG TCCTGTACAC GGCAGACCAG 
ACCAACAATG GCAAACTCAC CATCAACCTG CCCAAAGTGC CCACCTCTGC TCCTGTCAGG 
TGTTATTACA TTGTGGTCGT GCCCGTCTCT CAGACATCAT CTCGTCAGTG GATTAATCCC 
GATGAGATGG AGCTGGATGA GCTCCTGGAG TCTAGTGAGG GAGCTCATCT CAGGAGACGT 
CGACGGCACA CAGACACCGT AAGACCGTAC ATCGCCGCAA AGCTATCCAC GCTCCCAGAA 
ACCTTCACTC TGGGCGATGA GCTAGACTAC AGCGGCTTCA TCAACCGGCC TCTTCCCAAC 
AACCAACACT TCCAGTGTTT TGTGATGGCT GAACTCAAGG ACCAATACCC TGTTACTGCC 
AATGAGAAGC AGCGGACCTT CTCGGCTAGC CCATACTCAG ACCCCATCAT AGTGCAGGGT 
GAAAACCAAA TGCAGCGCAG TGTGGACCAA CCGGAGATGC TTTGGGTCAT GGGCCCTGTG 
CTCGCTGTGG TGCTCATCAT CATCATAGTC ATCGCCATCC TGCTCTTCAA AAGCAAACAG 
GAGAGGAAGA GGGCTTCCCC CCTACCAAAA GACGATCATT CGGGTGGTGT GAAGGATTGT 
CTTCTGGCCA GCTCATCCGA TCCTGTCGAA ATGAGGAGAC TCAACTACCA GACACCAGGA 
ATGAGGGAAC ATCCTCCCAT ATCCGTCTCC GAACTGGCAG ACCACATCGA GCGCCTCAAG 
GCCAACGATG CCCTCCGGTT TTCCCAAGAG TATGAGTCTA TTGATCCAGG CCAGCAGTTC 
ACATGGGAGA ACTCCAACTT GGAGGTCAAC AAGCCAAAGA ACCGTTATGC CAACGTTATT 
GCTTACGACC ACTCGAGAGT TGTTCTCACC CCTGTGGACG GTGTACCAGG GAGTGACTAC 
ATTAATTCCA ACTACATTGA TGGCTACCGT AAGCAGAATG CCTACATTGC CACACAAGGT 
CCTCTGCCTG AGACTCTGAG CGACTTCTGG AGGATGATTT GGGAGCAACG AGCCAGTACC 
ATCGTCATGA TGACCAGACT GGAAGAGAAG TCCAGAGTCA AGTGTGACCA ATACTGGCCC 
ATTCGTGGCA CAGAGACTTA TGGAATGATT CAAGTGACCA TGCTTGATGC TGTTGAGCTG 
GCAACCTACA GTGTGCGCAC CTTTGCCCTT TACAAGAATG GCTCTAGTGA GAAGCGCGAG 
GTCAGGCAGT TTCAGTTCAT GGCTTGGCCG GACCACGGAG TGCCTGAATA TCCCACCCCC 
ATCCTAGCCT TCCTGCGCAG GGTCAAAGCC TGCAACCCTC CTGATGCTGG ACCCATGGTA 
GTGCACTGCA GTGCTGGAGT TGGCCGTACA GGCTGCTTCA TAGTCATCGA TGCGATGTTG 
GAGCGCATGA AGCACGAGAA GACCGTAGAC ATTTATGGCC ACGTCACGTG CATGAGATCC 
CAGAGGAACT ATATGGTGCA GACGGAGGAC CAGTACATCT TTATCCACGA GGCCCTTCTG 
GAGGCCGCCA CCTGTGGAAA CACAGAGGTC CCTGCCCGAA ACCTCTACGC CCACATCCAG 
AAGCTCACAC AGCCATCGGC TGGAGAGACC GTCACAGCCA TGGAGCTGGA GTTTAAGAGA 
CTCGCCAACT CCAAAGCCCA CACCTCAAGA TTCATCAGCG CCAACCTGCC CTGCAACAAG 
TTCAAGAACC GGCTGGTCAA TATCATGCCT TTCGAGTCCA CACGGGTGTG CCTGCAGCCA 
ATCCGAGGAG TGGAGGGGTC CGACTACATC AATGCCAGCT GCATTGACGG ATACAGACAG 
CAGAAGGCCT ATATTGCCAC TCAGGGTCCT CTAGCGGAGA CCACCGAGGA CTTCTGGAGG 
ATGTTATGGG AGCACAATTC CACTATTGTA GTGATGCTGA CCAAACTCAG AGAGATGGGA 
CGGGAGAAGT GTCATCAGTA CTGGCCCGCC GAGCGCTCAG CCCGCTATCA GTACTTCGTA 
GTGGATCCCA TGGCCGAATA CAACATGCCC CAGTACATCC TGCGAGAGTT CAAAGTCACA 
GATGCCAGGG ACGGTCAATC CAGAACCATT CGCCAGTTCC AGTTCACTGA TTGGCCAGAG 
CAGGGAGTGC CAAAAACCGG TGAGGGGTTC ATCGATTTCA TTGGGCAAGT TCATAAAACC 
AAGGAGCAGT TTGGGCAGGA TGGACCAATC ACTGTGCACT GCAGTGCTGG AGTGGGAAGA 
ACAGGTGTTT TCATCACTCT CAGTATTGTC CTGGAGAGGA TGAGATATGA GGGGGTGGTG 
GATCTGTTCC AGACGGTTAA AACCCTGCGC ACACAAAGGC CTGCCATGGT GCAGACAGAG 
GACCAGTACC AGCTGTGCTA CAGAGCCGCT CTGGAGTACC TGGGGAGCTT TGATCACTAT 
GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296744.1
CDS	421..5829
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298469.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298469.3

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGTGCCAGG AGCACCGCCT CGGAAATTGG AAGTGGAGGC TTTGAACTCC 
ACCGCTATCC GTGTGACATG GAAACCTCCT CTTTCTGGAA AGCAGCATGG CCAGATCCGG 
GGATATCAGG TCATATACTC CCGGTTGGAG AACGGGGAAC CACGGGGACA TCCGAATATA 
ATGGATGTCG CTCTGCCAGA AGCACAGGAG GCTGTGATCG CTGGACTTGC CTCTGAAACC 
TCTTACTCAG TGACTGTGGC TGCGTACACA ACCAAAGGTG ATGGAGCTCG CAGCAAAGCC 
AAAGTGGTCA CCACCACCGG AGCAGTGCCT GGCAAACCCA CTATGATCAT CAGCACCACC 
GTTGGCAACA CGGCCCTTAT CCAGTGGCAG CCTCCTAAAG ACATGGTGGG TGAGCTGATG 
GGCTACCGGC TGCGCTACAA ACGTCTGGAA GAGGAAAACT ATGAGATACG CGAATTCAGT 
CGCACCGACG ACCACCACAC CGTCACTGAC CTGCACAAGG GCGCCACCTA CAGCTTCCAC 
CTGAGCGCCA GAAACCGCGC TGGACTCGGA GAGGAGTACG TTAAAGACAT CGGCACTCCT 
GAGGACGTGC CCAGCGGGTT TCCTCTTAAC CTGCAGGTCG TAGGCTTGAC TACCTCTACC 
ACCCGACTGA CCTGGGAGCC TCCAGCGTTG TCTGAACGTA ACGGACGGAT CATACATTAC 
ATTGTGGTTT ACCGAGACAT CAACAGCAAA CAAAACAGCA CCAACCGAAC CTCCGACACA 
CAGATGACCA TCCAAGGGCT CCGACCTGAC ACCACCTATG ATATCCGTGT GCAGGCGTTC 
ACTAGCAAGG GTGGAGGTCC CATAAGTCCC AGTATCCAGA GCAGGACCAT GTCTACGTCA 
CCTGCATTTG CCACAAGTTT TGGTGTCAAA GCTGTGATGA AAACCTCTGT CCTTCTCACC 
TGGGAGGTGC CAGAAAATTA TAAATCACAA GTGCCTTTTA AGATCCTCTA TAACCAGCAG 
AGTGTGGAAG TCCAGGGGAA TCTGAAGAGG AAGCTGATCA CACGCCTGCA GCCGGACACT 
GATTACTCGT TTGTGCTGAT GAGCCGGGGA AACAGCGCCG GTGGCCTACA ACAGCAGGTC 
TCCATCCGCA CTGCACCAGA CCTGTTCAAA ACTAAACCTG TCCTGTACAC GGCAGACCAG 
ACCAACAATG GCAAACTCAC CATCAACCTG CCCAAAGTGC CCACCTCTGC TCCTGTCAGG 
TGTTATTACA TTGTGGTCGT GCCCGTCTCT CAGACATCAT CTCGTCAGTG GATTAATCCC 
GATGAGATGG AGCTGGATGA GCTCCTGGAG TCTAGTGAGG GAGCTCATCT CAGGAGACGT 
CGACGGCACA CAGACACCGT AAGACCGTAC ATCGCCGCAA AGCTATCCAC GCTCCCAGAA 
ACCTTCACTC TGGGCGATGA GCTAGACTAC AGCGGCTTCA TCAACCGGCC TCTTCCCAAC 
AACCAACACT TCCAGTGTTT TGTGATGGCT GAACTCAAGG ACCAATACCC TGTTACTGCC 
AATGAGAAGC AGCGGACCTT CTCGGCTAGC CCATACTCAG ACCCCATCAT AGTGCAGGGT 
GAAAACCAAA TGCAGCGCAG TGTGGACCAA CCGGAGATGC TTTGGGTCAT GGGCCCTGTG 
CTCGCTGTGG TGCTCATCAT CATCATAGTC ATCGCCATCC TGCTCTTCAA AAGCAAACAG 
GAGAGGAAGA GGGCTTCCCC CCTACCAAAA GACGATCATT CGGGTGGTGT GAAGGATTGT 
CTTCTGGCCA GCTCATCCGA TCCTGTCGAA ATGAGGAGAC TCAACTACCA GACACCAGGA 
ATGAGGGAAC ATCCTCCCAT ATCCGTCTCC GAACTGGCAG ACCACATCGA GCGCCTCAAG 
GCCAACGATG CCCTCCGGTT TTCCCAAGAG TATGAGTCTA TTGATCCAGG CCAGCAGTTC 
ACATGGGAGA ACTCCAACTT GGAGGTCAAC AAGCCAAAGA ACCGTTATGC CAACGTTATT 
GCTTACGACC ACTCGAGAGT TGTTCTCACC CCTGTGGACG GTGTACCAGG GAGTGACTAC 
ATTAATTCCA ACTACATTGA TGGCTACCGT AAGCAGAATG CCTACATTGC CACACAAGGT 
CCTCTGCCTG AGACTCTGAG CGACTTCTGG AGGATGATTT GGGAGCAACG AGCCAGTACC 
ATCGTCATGA TGACCAGACT GGAAGAGAAG TCCAGAGTCA AGTGTGACCA ATACTGGCCC 
ATTCGTGGCA CAGAGACTTA TGGAATGATT CAAGTGACCA TGCTTGATGC TGTTGAGCTG 
GCAACCTACA GTGTGCGCAC CTTTGCCCTT TACAAGAATG GCTCTAGTGA GAAGCGCGAG 
GTCAGGCAGT TTCAGTTCAT GGCTTGGCCG GACCACGGAG TGCCTGAATA TCCCACCCCC 
ATCCTAGCCT TCCTGCGCAG GGTCAAAGCC TGCAACCCTC CTGATGCTGG ACCCATGGTA 
GTGCACTGCA GTGCTGGAGT TGGCCGTACA GGCTGCTTCA TAGTCATCGA TGCGATGTTG 
GAGCGCATGA AGCACGAGAA GACCGTAGAC ATTTATGGCC ACGTCACGTG CATGAGATCC 
CAGAGGAACT ATATGGTGCA GACGGAGGAC CAGTACATCT TTATCCACGA GGCCCTTCTG 
GAGGCCGCCA CCTGTGGAAA CACAGAGGTC CCTGCCCGAA ACCTCTACGC CCACATCCAG 
AAGCTCACAC AGCCATCGGC TGGAGAGACC GTCACAGCCA TGGAGCTGGA GTTTAAGAGA 
CTCGCCAACT CCAAAGCCCA CACCTCAAGA TTCATCAGCG CCAACCTGCC CTGCAACAAG 
TTCAAGAACC GGCTGGTCAA TATCATGCCT TTCGAGTCCA CACGGGTGTG CCTGCAGCCA 
ATCCGAGGAG TGGAGGGGTC CGACTACATC AATGCCAGCT GCATTGACGG ATACAGACAG 
CAGAAGGCCT ATATTGCCAC TCAGGGTCCT CTAGCGGAGA CCACCGAGGA CTTCTGGAGG 
ATGTTATGGG AGCACAATTC CACTATTGTA GTGATGCTGA CCAAACTCAG AGAGATGGGA 
CGGGAGAAGT GTCATCAGTA CTGGCCCGCC GAGCGCTCAG CCCGCTATCA GTACTTCGTA 
GTGGATCCCA TGGCCGAATA CAACATGCCC CAGTACATCC TGCGAGAGTT CAAAGTCACA 
GATGCCAGGG ACGGTCAATC CAGAACCATT CGCCAGTTCC AGTTCACTGA TTGGCCAGAG 
CAGGGAGTGC CAAAAACCGG TGAGGGGTTC ATCGATTTCA TTGGGCAAGT TCATAAAACC 
AAGGAGCAGT TTGGGCAGGA TGGACCAATC ACTGTGCACT GCAGTGCTGG AGTGGGAAGA 
ACAGGTGTTT TCATCACTCT CAGTATTGTC CTGGAGAGGA TGAGATATGA GGGGGTGGTG 
GATCTGTTCC AGACGGTTAA AACCCTGCGC ACACAAAGGC CTGCCATGGT GCAGACAGAG 
GACCAGTACC AGCTGTGCTA CAGAGCCGCT CTGGAGTACC TGGGGAGCTT TGATCACTAT 
GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_021481440.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337115.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X17, mRNA.

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CloneID	ODa47424
Clone ID Related Accession (Same CDS sequence)	XM_021481440.1 , XM_009298470.2
Accession Version	XM_021481440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGTGCCAGG AGCACCGCCT CGGAAATTGG AAGTGGAGGC TTTGAACTCC 
ACCGCTATCC GTGTGACATG GAAACCTCCT CTTTCTGGAA AGCAGCATGG CCAGATCCGG 
GGATATCAGG TCATATACTC CCGGTTGGAG AACGGGGAAC CACGGGGACA TCCGAATATA 
ATGGATGTCG CTCTGCCAGA AGCACAGGAG GCTGTGATCG CTGGACTTGC CTCTGAAACC 
TCTTACTCAG TGACTGTGGC TGCGTACACA ACCAAAGGTG ATGGAGCTCG CAGCAAAGCC 
AAAGTGGTCA CCACCACCGG AGCAGTGCCT GGCAAACCCA CTATGATCAT CAGCACCACC 
GTTGGCAACA CGGCCCTTAT CCAGTGGCAG CCTCCTAAAG ACATGGTGGG TGAGCTGATG 
GGCTACCGGC TGCGCTACAA ACGTCTGGAA GAGGAAAACT ATGAGATACG CGAATTCAGT 
CGCACCGACG ACCACCACAC CGTCACTGAC CTGCACAAGG GCGCCACCTA CAGCTTCCAC 
CTGAGCGCCA GAAACCGCGC TGGACTCGGA GAGGAGTACG TTAAAGACAT CGGCACTCCT 
GAGGACGTGC CCAGCGGGTT TCCTCTTAAC CTGCAGGTCG TAGGCTTGAC TACCTCTACC 
ACCCGACTGA CCTGGGAGCC TCCAGCGTTG TCTGAACGTA ACGGACGGAT CATACATTAC 
ATTGTGGTTT ACCGAGACAT CAACAGCAAA CAAAACAGCA CCAACCGAAC CTCCGACACA 
CAGATGACCA TCCAAGGGCT CCGACCTGAC ACCACCTATG ATATCCGTGT GCAGGCGTTC 
ACTAGCAAGG GTGGAGGTCC CATAAGTCCC AGTATCCAGA GCAGGACCAT GTCTACGTCA 
CCTGCATTTG CCACAAGTTT TGGTGTCAAA GCTGTGATGA AAACCTCTGT CCTTCTCACC 
TGGGAGGTGC CAGAAAATTA TAAATCACAA GTGCCTTTTA AGATCCTCTA TAACCAGCAG 
AGTGTGGAAG TCCAGGGGAA TCTGAAGAGG AAGCTGATCA CACGCCTGCA GCCGGACACT 
GATTACTCGT TTGTGCTGAT GAGCCGGGGA AACAGCGCCG GTGGCCTACA ACAGCAGGTC 
TCCATCCGCA CTGCACCAGA CCTGTTCAAA ACTAAACCTG TCCTGTACAC GGCAGACCAG 
ACCAACAATG GCAAACTCAC CATCAACCTG CCCAAAGTGC CCACCTCTGC TCCTGTCAGG 
TGTTATTACA TTGTGGTCGT GCCCGTCTCT CAGACATCAT CTCGTCAGTG GATTAATCCC 
GATGAGATGG AGCTGGATGA GCTCCTGGAG TCTAGTGAGG GAGCTCATCT CAGGAGACGT 
CGACGGCACA CAGACACCGT AAGACCGTAC ATCGCCGCAA AGCTATCCAC GCTCCCAGAA 
ACCTTCACTC TGGGCGATGA GCTAGACTAC AGCGGCTTCA TCAACCGGCC TCTTCCCAAC 
AACCAACACT TCCAGTGTTT TGTGATGGCT GAACTCAAGG ACCAATACCC TGTTACTGCC 
AATGAGAAGC AGCGGACCTT CTCGGCTAGC CCATACTCAG ACCCCATCAT AGTGCAGGGT 
GAAAACCAAA TGCAGCGCAG TGTGGACCAA CCGGAGATGC TTTGGGTCAT GGGCCCTGTG 
CTCGCTGTGG TGCTCATCAT CATCATAGTC ATCGCCATCC TGCTCTTCAA AAGGAAGAGG 
GCTTCCCCCC TACCAAAAGA CGATCATTCG GGTGGTGTGA AGGATTGTCT TCTGGCCAGC 
TCATCCGATC CTGTCGAAAT GAGGAGACTC AACTACCAGA CACCAGGAAT GAGGGAACAT 
CCTCCCATAT CCGTCTCCGA ACTGGCAGAC CACATCGAGC GCCTCAAGGC CAACGATGCC 
CTCCGGTTTT CCCAAGAGTA TGAGTCTATT GATCCAGGCC AGCAGTTCAC ATGGGAGAAC 
TCCAACTTGG AGGTCAACAA GCCAAAGAAC CGTTATGCCA ACGTTATTGC TTACGACCAC 
TCGAGAGTTG TTCTCACCCC TGTGGACGGT GTACCAGGGA GTGACTACAT TAATTCCAAC 
TACATTGATG GCTACCGTAA GCAGAATGCC TACATTGCCA CACAAGGTCC TCTGCCTGAG 
ACTCTGAGCG ACTTCTGGAG GATGATTTGG GAGCAACGAG CCAGTACCAT CGTCATGATG 
ACCAGACTGG AAGAGAAGTC CAGAGTCAAG TGTGACCAAT ACTGGCCCAT TCGTGGCACA 
GAGACTTATG GAATGATTCA AGTGACCATG CTTGATGCTG TTGAGCTGGC AACCTACAGT 
GTGCGCACCT TTGCCCTTTA CAAGAATGGC TCTAGTGAGA AGCGCGAGGT CAGGCAGTTT 
CAGTTCATGG CTTGGCCGGA CCACGGAGTG CCTGAATATC CCACCCCCAT CCTAGCCTTC 
CTGCGCAGGG TCAAAGCCTG CAACCCTCCT GATGCTGGAC CCATGGTAGT GCACTGCAGT 
GCTGGAGTTG GCCGTACAGG CTGCTTCATA GTCATCGATG CGATGTTGGA GCGCATGAAG 
CACGAGAAGA CCGTAGACAT TTATGGCCAC GTCACGTGCA TGAGATCCCA GAGGAACTAT 
ATGGTGCAGA CGGAGGACCA GTACATCTTT ATCCACGAGG CCCTTCTGGA GGCCGCCACC 
TGTGGAAACA CAGAGGTCCC TGCCCGAAAC CTCTACGCCC ACATCCAGAA GCTCACACAG 
CCATCGGCTG GAGAGACCGT CACAGCCATG GAGCTGGAGT TTAAGAGACT CGCCAACTCC 
AAAGCCCACA CCTCAAGATT CATCAGCGCC AACCTGCCCT GCAACAAGTT CAAGAACCGG 
CTGGTCAATA TCATGCCTTT CGAGTCCACA CGGGTGTGCC TGCAGCCAAT CCGAGGAGTG 
GAGGGGTCCG ACTACATCAA TGCCAGCTGC ATTGACGGAT ACAGACAGCA GAAGGCCTAT 
ATTGCCACTC AGGGTCCTCT AGCGGAGACC ACCGAGGACT TCTGGAGGAT GTTATGGGAG 
CACAATTCCA CTATTGTAGT GATGCTGACC AAACTCAGAG AGATGGGACG GGAGAAGTGT 
CATCAGTACT GGCCCGCCGA GCGCTCAGCC CGCTATCAGT ACTTCGTAGT GGATCCCATG 
GCCGAATACA ACATGCCCCA GTACATCCTG CGAGAGTTCA AAGTCACAGA TGCCAGGGAC 
GGTCAATCCA GAACCATTCG CCAGTTCCAG TTCACTGATT GGCCAGAGCA GGGAGTGCCA 
AAAACCGGTG AGGGGTTCAT CGATTTCATT GGGCAAGTTC ATAAAACCAA GGAGCAGTTT 
GGGCAGGATG GACCAATCAC TGTGCACTGC AGTGCTGGAG TGGGAAGAAC AGGTGTTTTC 
ATCACTCTCA GTATTGTCCT GGAGAGGATG AGATATGAGG GGGTGGTGGA TCTGTTCCAG 
ACGGTTAAAA CCCTGCGCAC ACAAAGGCCT GCCATGGTGC AGACAGAGGA CCAGTACCAG 
CTGTGCTACA GAGCCGCTCT GGAGTACCTG GGGAGCTTTG ATCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337115.1
CDS	421..5817
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKRKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021481440.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481440.1

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGTGCCAGG AGCACCGCCT CGGAAATTGG AAGTGGAGGC TTTGAACTCC 
ACCGCTATCC GTGTGACATG GAAACCTCCT CTTTCTGGAA AGCAGCATGG CCAGATCCGG 
GGATATCAGG TCATATACTC CCGGTTGGAG AACGGGGAAC CACGGGGACA TCCGAATATA 
ATGGATGTCG CTCTGCCAGA AGCACAGGAG GCTGTGATCG CTGGACTTGC CTCTGAAACC 
TCTTACTCAG TGACTGTGGC TGCGTACACA ACCAAAGGTG ATGGAGCTCG CAGCAAAGCC 
AAAGTGGTCA CCACCACCGG AGCAGTGCCT GGCAAACCCA CTATGATCAT CAGCACCACC 
GTTGGCAACA CGGCCCTTAT CCAGTGGCAG CCTCCTAAAG ACATGGTGGG TGAGCTGATG 
GGCTACCGGC TGCGCTACAA ACGTCTGGAA GAGGAAAACT ATGAGATACG CGAATTCAGT 
CGCACCGACG ACCACCACAC CGTCACTGAC CTGCACAAGG GCGCCACCTA CAGCTTCCAC 
CTGAGCGCCA GAAACCGCGC TGGACTCGGA GAGGAGTACG TTAAAGACAT CGGCACTCCT 
GAGGACGTGC CCAGCGGGTT TCCTCTTAAC CTGCAGGTCG TAGGCTTGAC TACCTCTACC 
ACCCGACTGA CCTGGGAGCC TCCAGCGTTG TCTGAACGTA ACGGACGGAT CATACATTAC 
ATTGTGGTTT ACCGAGACAT CAACAGCAAA CAAAACAGCA CCAACCGAAC CTCCGACACA 
CAGATGACCA TCCAAGGGCT CCGACCTGAC ACCACCTATG ATATCCGTGT GCAGGCGTTC 
ACTAGCAAGG GTGGAGGTCC CATAAGTCCC AGTATCCAGA GCAGGACCAT GTCTACGTCA 
CCTGCATTTG CCACAAGTTT TGGTGTCAAA GCTGTGATGA AAACCTCTGT CCTTCTCACC 
TGGGAGGTGC CAGAAAATTA TAAATCACAA GTGCCTTTTA AGATCCTCTA TAACCAGCAG 
AGTGTGGAAG TCCAGGGGAA TCTGAAGAGG AAGCTGATCA CACGCCTGCA GCCGGACACT 
GATTACTCGT TTGTGCTGAT GAGCCGGGGA AACAGCGCCG GTGGCCTACA ACAGCAGGTC 
TCCATCCGCA CTGCACCAGA CCTGTTCAAA ACTAAACCTG TCCTGTACAC GGCAGACCAG 
ACCAACAATG GCAAACTCAC CATCAACCTG CCCAAAGTGC CCACCTCTGC TCCTGTCAGG 
TGTTATTACA TTGTGGTCGT GCCCGTCTCT CAGACATCAT CTCGTCAGTG GATTAATCCC 
GATGAGATGG AGCTGGATGA GCTCCTGGAG TCTAGTGAGG GAGCTCATCT CAGGAGACGT 
CGACGGCACA CAGACACCGT AAGACCGTAC ATCGCCGCAA AGCTATCCAC GCTCCCAGAA 
ACCTTCACTC TGGGCGATGA GCTAGACTAC AGCGGCTTCA TCAACCGGCC TCTTCCCAAC 
AACCAACACT TCCAGTGTTT TGTGATGGCT GAACTCAAGG ACCAATACCC TGTTACTGCC 
AATGAGAAGC AGCGGACCTT CTCGGCTAGC CCATACTCAG ACCCCATCAT AGTGCAGGGT 
GAAAACCAAA TGCAGCGCAG TGTGGACCAA CCGGAGATGC TTTGGGTCAT GGGCCCTGTG 
CTCGCTGTGG TGCTCATCAT CATCATAGTC ATCGCCATCC TGCTCTTCAA AAGGAAGAGG 
GCTTCCCCCC TACCAAAAGA CGATCATTCG GGTGGTGTGA AGGATTGTCT TCTGGCCAGC 
TCATCCGATC CTGTCGAAAT GAGGAGACTC AACTACCAGA CACCAGGAAT GAGGGAACAT 
CCTCCCATAT CCGTCTCCGA ACTGGCAGAC CACATCGAGC GCCTCAAGGC CAACGATGCC 
CTCCGGTTTT CCCAAGAGTA TGAGTCTATT GATCCAGGCC AGCAGTTCAC ATGGGAGAAC 
TCCAACTTGG AGGTCAACAA GCCAAAGAAC CGTTATGCCA ACGTTATTGC TTACGACCAC 
TCGAGAGTTG TTCTCACCCC TGTGGACGGT GTACCAGGGA GTGACTACAT TAATTCCAAC 
TACATTGATG GCTACCGTAA GCAGAATGCC TACATTGCCA CACAAGGTCC TCTGCCTGAG 
ACTCTGAGCG ACTTCTGGAG GATGATTTGG GAGCAACGAG CCAGTACCAT CGTCATGATG 
ACCAGACTGG AAGAGAAGTC CAGAGTCAAG TGTGACCAAT ACTGGCCCAT TCGTGGCACA 
GAGACTTATG GAATGATTCA AGTGACCATG CTTGATGCTG TTGAGCTGGC AACCTACAGT 
GTGCGCACCT TTGCCCTTTA CAAGAATGGC TCTAGTGAGA AGCGCGAGGT CAGGCAGTTT 
CAGTTCATGG CTTGGCCGGA CCACGGAGTG CCTGAATATC CCACCCCCAT CCTAGCCTTC 
CTGCGCAGGG TCAAAGCCTG CAACCCTCCT GATGCTGGAC CCATGGTAGT GCACTGCAGT 
GCTGGAGTTG GCCGTACAGG CTGCTTCATA GTCATCGATG CGATGTTGGA GCGCATGAAG 
CACGAGAAGA CCGTAGACAT TTATGGCCAC GTCACGTGCA TGAGATCCCA GAGGAACTAT 
ATGGTGCAGA CGGAGGACCA GTACATCTTT ATCCACGAGG CCCTTCTGGA GGCCGCCACC 
TGTGGAAACA CAGAGGTCCC TGCCCGAAAC CTCTACGCCC ACATCCAGAA GCTCACACAG 
CCATCGGCTG GAGAGACCGT CACAGCCATG GAGCTGGAGT TTAAGAGACT CGCCAACTCC 
AAAGCCCACA CCTCAAGATT CATCAGCGCC AACCTGCCCT GCAACAAGTT CAAGAACCGG 
CTGGTCAATA TCATGCCTTT CGAGTCCACA CGGGTGTGCC TGCAGCCAAT CCGAGGAGTG 
GAGGGGTCCG ACTACATCAA TGCCAGCTGC ATTGACGGAT ACAGACAGCA GAAGGCCTAT 
ATTGCCACTC AGGGTCCTCT AGCGGAGACC ACCGAGGACT TCTGGAGGAT GTTATGGGAG 
CACAATTCCA CTATTGTAGT GATGCTGACC AAACTCAGAG AGATGGGACG GGAGAAGTGT 
CATCAGTACT GGCCCGCCGA GCGCTCAGCC CGCTATCAGT ACTTCGTAGT GGATCCCATG 
GCCGAATACA ACATGCCCCA GTACATCCTG CGAGAGTTCA AAGTCACAGA TGCCAGGGAC 
GGTCAATCCA GAACCATTCG CCAGTTCCAG TTCACTGATT GGCCAGAGCA GGGAGTGCCA 
AAAACCGGTG AGGGGTTCAT CGATTTCATT GGGCAAGTTC ATAAAACCAA GGAGCAGTTT 
GGGCAGGATG GACCAATCAC TGTGCACTGC AGTGCTGGAG TGGGAAGAAC AGGTGTTTTC 
ATCACTCTCA GTATTGTCCT GGAGAGGATG AGATATGAGG GGGTGGTGGA TCTGTTCCAG 
ACGGTTAAAA CCCTGCGCAC ACAAAGGCCT GCCATGGTGC AGACAGAGGA CCAGTACCAG 
CTGTGCTACA GAGCCGCTCT GGAGTACCTG GGGAGCTTTG ATCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfb ( XM_009298470.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296745.1 Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X13, mRNA.

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CloneID	ODa47424
Clone ID Related Accession (Same CDS sequence)	XM_021481440.1 , XM_009298470.2
Accession Version	XM_009298470.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298470.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGTGCCAGG AGCACCGCCT CGGAAATTGG AAGTGGAGGC TTTGAACTCC 
ACCGCTATCC GTGTGACATG GAAACCTCCT CTTTCTGGAA AGCAGCATGG CCAGATCCGG 
GGATATCAGG TCATATACTC CCGGTTGGAG AACGGGGAAC CACGGGGACA TCCGAATATA 
ATGGATGTCG CTCTGCCAGA AGCACAGGAG GCTGTGATCG CTGGACTTGC CTCTGAAACC 
TCTTACTCAG TGACTGTGGC TGCGTACACA ACCAAAGGTG ATGGAGCTCG CAGCAAAGCC 
AAAGTGGTCA CCACCACCGG AGCAGTGCCT GGCAAACCCA CTATGATCAT CAGCACCACC 
GTTGGCAACA CGGCCCTTAT CCAGTGGCAG CCTCCTAAAG ACATGGTGGG TGAGCTGATG 
GGCTACCGGC TGCGCTACAA ACGTCTGGAA GAGGAAAACT ATGAGATACG CGAATTCAGT 
CGCACCGACG ACCACCACAC CGTCACTGAC CTGCACAAGG GCGCCACCTA CAGCTTCCAC 
CTGAGCGCCA GAAACCGCGC TGGACTCGGA GAGGAGTACG TTAAAGACAT CGGCACTCCT 
GAGGACGTGC CCAGCGGGTT TCCTCTTAAC CTGCAGGTCG TAGGCTTGAC TACCTCTACC 
ACCCGACTGA CCTGGGAGCC TCCAGCGTTG TCTGAACGTA ACGGACGGAT CATACATTAC 
ATTGTGGTTT ACCGAGACAT CAACAGCAAA CAAAACAGCA CCAACCGAAC CTCCGACACA 
CAGATGACCA TCCAAGGGCT CCGACCTGAC ACCACCTATG ATATCCGTGT GCAGGCGTTC 
ACTAGCAAGG GTGGAGGTCC CATAAGTCCC AGTATCCAGA GCAGGACCAT GTCTACGTCA 
CCTGCATTTG CCACAAGTTT TGGTGTCAAA GCTGTGATGA AAACCTCTGT CCTTCTCACC 
TGGGAGGTGC CAGAAAATTA TAAATCACAA GTGCCTTTTA AGATCCTCTA TAACCAGCAG 
AGTGTGGAAG TCCAGGGGAA TCTGAAGAGG AAGCTGATCA CACGCCTGCA GCCGGACACT 
GATTACTCGT TTGTGCTGAT GAGCCGGGGA AACAGCGCCG GTGGCCTACA ACAGCAGGTC 
TCCATCCGCA CTGCACCAGA CCTGTTCAAA ACTAAACCTG TCCTGTACAC GGCAGACCAG 
ACCAACAATG GCAAACTCAC CATCAACCTG CCCAAAGTGC CCACCTCTGC TCCTGTCAGG 
TGTTATTACA TTGTGGTCGT GCCCGTCTCT CAGACATCAT CTCGTCAGTG GATTAATCCC 
GATGAGATGG AGCTGGATGA GCTCCTGGAG TCTAGTGAGG GAGCTCATCT CAGGAGACGT 
CGACGGCACA CAGACACCGT AAGACCGTAC ATCGCCGCAA AGCTATCCAC GCTCCCAGAA 
ACCTTCACTC TGGGCGATGA GCTAGACTAC AGCGGCTTCA TCAACCGGCC TCTTCCCAAC 
AACCAACACT TCCAGTGTTT TGTGATGGCT GAACTCAAGG ACCAATACCC TGTTACTGCC 
AATGAGAAGC AGCGGACCTT CTCGGCTAGC CCATACTCAG ACCCCATCAT AGTGCAGGGT 
GAAAACCAAA TGCAGCGCAG TGTGGACCAA CCGGAGATGC TTTGGGTCAT GGGCCCTGTG 
CTCGCTGTGG TGCTCATCAT CATCATAGTC ATCGCCATCC TGCTCTTCAA AAGGAAGAGG 
GCTTCCCCCC TACCAAAAGA CGATCATTCG GGTGGTGTGA AGGATTGTCT TCTGGCCAGC 
TCATCCGATC CTGTCGAAAT GAGGAGACTC AACTACCAGA CACCAGGAAT GAGGGAACAT 
CCTCCCATAT CCGTCTCCGA ACTGGCAGAC CACATCGAGC GCCTCAAGGC CAACGATGCC 
CTCCGGTTTT CCCAAGAGTA TGAGTCTATT GATCCAGGCC AGCAGTTCAC ATGGGAGAAC 
TCCAACTTGG AGGTCAACAA GCCAAAGAAC CGTTATGCCA ACGTTATTGC TTACGACCAC 
TCGAGAGTTG TTCTCACCCC TGTGGACGGT GTACCAGGGA GTGACTACAT TAATTCCAAC 
TACATTGATG GCTACCGTAA GCAGAATGCC TACATTGCCA CACAAGGTCC TCTGCCTGAG 
ACTCTGAGCG ACTTCTGGAG GATGATTTGG GAGCAACGAG CCAGTACCAT CGTCATGATG 
ACCAGACTGG AAGAGAAGTC CAGAGTCAAG TGTGACCAAT ACTGGCCCAT TCGTGGCACA 
GAGACTTATG GAATGATTCA AGTGACCATG CTTGATGCTG TTGAGCTGGC AACCTACAGT 
GTGCGCACCT TTGCCCTTTA CAAGAATGGC TCTAGTGAGA AGCGCGAGGT CAGGCAGTTT 
CAGTTCATGG CTTGGCCGGA CCACGGAGTG CCTGAATATC CCACCCCCAT CCTAGCCTTC 
CTGCGCAGGG TCAAAGCCTG CAACCCTCCT GATGCTGGAC CCATGGTAGT GCACTGCAGT 
GCTGGAGTTG GCCGTACAGG CTGCTTCATA GTCATCGATG CGATGTTGGA GCGCATGAAG 
CACGAGAAGA CCGTAGACAT TTATGGCCAC GTCACGTGCA TGAGATCCCA GAGGAACTAT 
ATGGTGCAGA CGGAGGACCA GTACATCTTT ATCCACGAGG CCCTTCTGGA GGCCGCCACC 
TGTGGAAACA CAGAGGTCCC TGCCCGAAAC CTCTACGCCC ACATCCAGAA GCTCACACAG 
CCATCGGCTG GAGAGACCGT CACAGCCATG GAGCTGGAGT TTAAGAGACT CGCCAACTCC 
AAAGCCCACA CCTCAAGATT CATCAGCGCC AACCTGCCCT GCAACAAGTT CAAGAACCGG 
CTGGTCAATA TCATGCCTTT CGAGTCCACA CGGGTGTGCC TGCAGCCAAT CCGAGGAGTG 
GAGGGGTCCG ACTACATCAA TGCCAGCTGC ATTGACGGAT ACAGACAGCA GAAGGCCTAT 
ATTGCCACTC AGGGTCCTCT AGCGGAGACC ACCGAGGACT TCTGGAGGAT GTTATGGGAG 
CACAATTCCA CTATTGTAGT GATGCTGACC AAACTCAGAG AGATGGGACG GGAGAAGTGT 
CATCAGTACT GGCCCGCCGA GCGCTCAGCC CGCTATCAGT ACTTCGTAGT GGATCCCATG 
GCCGAATACA ACATGCCCCA GTACATCCTG CGAGAGTTCA AAGTCACAGA TGCCAGGGAC 
GGTCAATCCA GAACCATTCG CCAGTTCCAG TTCACTGATT GGCCAGAGCA GGGAGTGCCA 
AAAACCGGTG AGGGGTTCAT CGATTTCATT GGGCAAGTTC ATAAAACCAA GGAGCAGTTT 
GGGCAGGATG GACCAATCAC TGTGCACTGC AGTGCTGGAG TGGGAAGAAC AGGTGTTTTC 
ATCACTCTCA GTATTGTCCT GGAGAGGATG AGATATGAGG GGGTGGTGGA TCTGTTCCAG 
ACGGTTAAAA CCCTGCGCAC ACAAAGGCCT GCCATGGTGC AGACAGAGGA CCAGTACCAG 
CTGTGCTACA GAGCCGCTCT GGAGTACCTG GGGAGCTTTG ATCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296745.1
CDS	74..5470
Translation	MVPNTCTSVPLLPVGLPLLLLLSCIQFSSQADSLPNFVRSPEDQTGISGGVASFVCQAAGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSLGEINTSAKLTVLEENQIPHGFPTIDMGPQLKVVERTRTTTMLCAASGNPDPEITWFKDFLPVDINGNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMTGEVELTKDEEMPVGRNVLELTNIRQSTNYTCVAISSLGMIEATAQVSVKALPKPPTSLTVTETTATSVTLTWDSGNPEPVSYYIIQYRAKTSDTNFQEVDGVATTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSEPVETRTGEQAPSSPPLHVQARMLSASTMLVQWEPPEEPNGQIRGYRIYYTSDLDAPLSAWQKHNTDDSRLTTISSLTTGITYSLRVLGFTSVGDGPPSDVLQVKTQQGVPAQPSSFEAEAELDTRILLTWLWPVQDPIIKYELQYWEADSDNKIHVTFDPAGSYAVEGLKPDTLYKFSLAARSEMGQGVFTHPIEARTAQSVPGAPPRKLEVEALNSTAIRVTWKPPLSGKQHGQIRGYQVIYSRLENGEPRGHPNIMDVALPEAQEAVIAGLASETSYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTVGNTALIQWQPPKDMVGELMGYRLRYKRLEEENYEIREFSRTDDHHTVTDLHKGATYSFHLSARNRAGLGEEYVKDIGTPEDVPSGFPLNLQVVGLTTSTTRLTWEPPALSERNGRIIHYIVVYRDINSKQNSTNRTSDTQMTIQGLRPDTTYDIRVQAFTSKGGGPISPSIQSRTMSTSPAFATSFGVKAVMKTSVLLTWEVPENYKSQVPFKILYNQQSVEVQGNLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFKTKPVLYTADQTNNGKLTINLPKVPTSAPVRCYYIVVVPVSQTSSRQWINPDEMELDELLESSEGAHLRRRRRHTDTVRPYIAAKLSTLPETFTLGDELDYSGFINRPLPNNQHFQCFVMAELKDQYPVTANEKQRTFSASPYSDPIIVQGENQMQRSVDQPEMLWVMGPVLAVVLIIIIVIAILLFKRKRASPLPKDDHSGGVKDCLLASSSDPVEMRRLNYQTPGMREHPPISVSELADHIERLKANDALRFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINSNYIDGYRKQNAYIATQGPLPETLSDFWRMIWEQRASTIVMMTRLEEKSRVKCDQYWPIRGTETYGMIQVTMLDAVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARNLYAHIQKLTQPSAGETVTAMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPFESTRVCLQPIRGVEGSDYINASCIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDLFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_009298470.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, b (ptprfb), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298470.2

ORF Insert Sequence:

ATGGTGCCTA ACACTTGCAC ATCCGTGCCC CTCCTGCCCG TCGGGCTTCC TCTTCTGCTA 
CTGCTGAGCT GCATACAATT CTCCTCTCAG GCAGACAGCT TGCCAAATTT CGTCAGATCT 
CCAGAGGACC AGACGGGGAT TTCAGGAGGA GTGGCGTCTT TTGTGTGCCA AGCAGCGGGT 
GAACCCAAGC CTCGCATCAC ATGGATGAAG AAAGGAAAGA AAGTCAGCTC GCAGCGCTTT 
GAGGTGATTG AGTTTGATGA TGGTTCGGGC TCAGTTCTGC GCATTCAGCC ACTGCGAACT 
CACAGAGACG AGGCCATCTA TGAGTGCACA GCCACCAACA GCCTGGGGGA AATCAACACC 
AGCGCCAAAC TCACCGTGCT GGAAGAAAAT CAGATTCCGC ATGGTTTCCC CACCATAGAC 
ATGGGTCCTC AGCTGAAAGT GGTGGAGCGA ACAAGAACAA CTACAATGCT CTGTGCTGCC 
AGTGGGAACC CTGACCCGGA GATCACTTGG TTTAAAGACT TCCTGCCTGT AGACATCAAC 
GGCAACGGGC GAATCAAACA GCTTCGCTCA GGAGCTCTGC AGATTGAAAA CAGCGAGGAA 
TCAGACCAGG GCAAGTACGA ATGTGTGGCT ACGAATAGCG CCGGGACGCG TTATTCAGCT 
CCGGCTAATC TCTACGTGAG AGTACGACGA GTTCCACCCC GTTTCTCCAT CCCACCTACC 
AATCACGAGG TGATGCCAGG GGGCAGTGTA AACCTCACCT GCGTGGCAGT AGGTGCCCCG 
ATGCCCTACG TCAAATGGAT GACTGGTGAG GTGGAGCTCA CCAAGGATGA GGAGATGCCT 
GTGGGACGCA ACGTCTTGGA GCTCACCAAC ATCCGTCAGT CGACCAACTA CACCTGCGTG 
GCTATATCAT CCCTAGGTAT GATCGAGGCA ACGGCACAAG TGTCTGTCAA AGCTCTCCCA 
AAGCCTCCTA CATCCCTGAC TGTTACGGAA ACCACAGCTA CGAGTGTTAC ACTCACATGG 
GACTCTGGAA ACCCTGAACC TGTGTCTTAC TACATAATTC AATACCGGGC CAAGACTTCA 
GACACCAACT TCCAAGAAGT GGACGGTGTG GCCACCACAC GTTACAGCAT CGGCGGTCTC 
AGCCCATATT CAGAGTATGA GTTTCGAGTA ATGGCAGTCA ACAACATTGG TCGTGGGCCA 
CCTAGTGAAC CCGTTGAAAC ACGAACAGGT GAACAGGCAC CTTCCTCCCC ACCGCTGCAT 
GTTCAAGCAC GAATGCTGAG TGCCAGCACC ATGTTGGTTC AGTGGGAACC ACCAGAGGAA 
CCCAATGGTC AGATCAGAGG CTATCGGATC TACTACACAT CTGACCTGGA TGCACCTCTT 
AGCGCCTGGC AGAAACACAA CACAGACGAT AGCCGTCTCA CCACTATCTC CAGCCTGACC 
ACCGGCATCA CCTACAGCCT ACGTGTGCTG GGCTTCACAT CTGTAGGGGA TGGACCACCA 
TCTGATGTGC TACAGGTCAA GACACAGCAA GGAGTTCCTG CTCAGCCATC CAGCTTCGAA 
GCTGAAGCAG AGCTAGATAC AAGGATCCTA CTAACTTGGC TGTGGCCCGT GCAGGACCCC 
ATCATCAAAT ACGAGCTGCA ATATTGGGAG GCTGACTCAG ACAACAAGAT CCATGTCACC 
TTTGACCCAG CTGGCTCGTA CGCTGTTGAA GGTCTGAAAC CAGACACTTT GTACAAATTC 
TCTCTGGCAG CCCGCTCTGA AATGGGACAA GGAGTCTTCA CTCACCCCAT TGAAGCCAGG 
ACGGCCCAGT CTGTGCCAGG AGCACCGCCT CGGAAATTGG AAGTGGAGGC TTTGAACTCC 
ACCGCTATCC GTGTGACATG GAAACCTCCT CTTTCTGGAA AGCAGCATGG CCAGATCCGG 
GGATATCAGG TCATATACTC CCGGTTGGAG AACGGGGAAC CACGGGGACA TCCGAATATA 
ATGGATGTCG CTCTGCCAGA AGCACAGGAG GCTGTGATCG CTGGACTTGC CTCTGAAACC 
TCTTACTCAG TGACTGTGGC TGCGTACACA ACCAAAGGTG ATGGAGCTCG CAGCAAAGCC 
AAAGTGGTCA CCACCACCGG AGCAGTGCCT GGCAAACCCA CTATGATCAT CAGCACCACC 
GTTGGCAACA CGGCCCTTAT CCAGTGGCAG CCTCCTAAAG ACATGGTGGG TGAGCTGATG 
GGCTACCGGC TGCGCTACAA ACGTCTGGAA GAGGAAAACT ATGAGATACG CGAATTCAGT 
CGCACCGACG ACCACCACAC CGTCACTGAC CTGCACAAGG GCGCCACCTA CAGCTTCCAC 
CTGAGCGCCA GAAACCGCGC TGGACTCGGA GAGGAGTACG TTAAAGACAT CGGCACTCCT 
GAGGACGTGC CCAGCGGGTT TCCTCTTAAC CTGCAGGTCG TAGGCTTGAC TACCTCTACC 
ACCCGACTGA CCTGGGAGCC TCCAGCGTTG TCTGAACGTA ACGGACGGAT CATACATTAC 
ATTGTGGTTT ACCGAGACAT CAACAGCAAA CAAAACAGCA CCAACCGAAC CTCCGACACA 
CAGATGACCA TCCAAGGGCT CCGACCTGAC ACCACCTATG ATATCCGTGT GCAGGCGTTC 
ACTAGCAAGG GTGGAGGTCC CATAAGTCCC AGTATCCAGA GCAGGACCAT GTCTACGTCA 
CCTGCATTTG CCACAAGTTT TGGTGTCAAA GCTGTGATGA AAACCTCTGT CCTTCTCACC 
TGGGAGGTGC CAGAAAATTA TAAATCACAA GTGCCTTTTA AGATCCTCTA TAACCAGCAG 
AGTGTGGAAG TCCAGGGGAA TCTGAAGAGG AAGCTGATCA CACGCCTGCA GCCGGACACT 
GATTACTCGT TTGTGCTGAT GAGCCGGGGA AACAGCGCCG GTGGCCTACA ACAGCAGGTC 
TCCATCCGCA CTGCACCAGA CCTGTTCAAA ACTAAACCTG TCCTGTACAC GGCAGACCAG 
ACCAACAATG GCAAACTCAC CATCAACCTG CCCAAAGTGC CCACCTCTGC TCCTGTCAGG 
TGTTATTACA TTGTGGTCGT GCCCGTCTCT CAGACATCAT CTCGTCAGTG GATTAATCCC 
GATGAGATGG AGCTGGATGA GCTCCTGGAG TCTAGTGAGG GAGCTCATCT CAGGAGACGT 
CGACGGCACA CAGACACCGT AAGACCGTAC ATCGCCGCAA AGCTATCCAC GCTCCCAGAA 
ACCTTCACTC TGGGCGATGA GCTAGACTAC AGCGGCTTCA TCAACCGGCC TCTTCCCAAC 
AACCAACACT TCCAGTGTTT TGTGATGGCT GAACTCAAGG ACCAATACCC TGTTACTGCC 
AATGAGAAGC AGCGGACCTT CTCGGCTAGC CCATACTCAG ACCCCATCAT AGTGCAGGGT 
GAAAACCAAA TGCAGCGCAG TGTGGACCAA CCGGAGATGC TTTGGGTCAT GGGCCCTGTG 
CTCGCTGTGG TGCTCATCAT CATCATAGTC ATCGCCATCC TGCTCTTCAA AAGGAAGAGG 
GCTTCCCCCC TACCAAAAGA CGATCATTCG GGTGGTGTGA AGGATTGTCT TCTGGCCAGC 
TCATCCGATC CTGTCGAAAT GAGGAGACTC AACTACCAGA CACCAGGAAT GAGGGAACAT 
CCTCCCATAT CCGTCTCCGA ACTGGCAGAC CACATCGAGC GCCTCAAGGC CAACGATGCC 
CTCCGGTTTT CCCAAGAGTA TGAGTCTATT GATCCAGGCC AGCAGTTCAC ATGGGAGAAC 
TCCAACTTGG AGGTCAACAA GCCAAAGAAC CGTTATGCCA ACGTTATTGC TTACGACCAC 
TCGAGAGTTG TTCTCACCCC TGTGGACGGT GTACCAGGGA GTGACTACAT TAATTCCAAC 
TACATTGATG GCTACCGTAA GCAGAATGCC TACATTGCCA CACAAGGTCC TCTGCCTGAG 
ACTCTGAGCG ACTTCTGGAG GATGATTTGG GAGCAACGAG CCAGTACCAT CGTCATGATG 
ACCAGACTGG AAGAGAAGTC CAGAGTCAAG TGTGACCAAT ACTGGCCCAT TCGTGGCACA 
GAGACTTATG GAATGATTCA AGTGACCATG CTTGATGCTG TTGAGCTGGC AACCTACAGT 
GTGCGCACCT TTGCCCTTTA CAAGAATGGC TCTAGTGAGA AGCGCGAGGT CAGGCAGTTT 
CAGTTCATGG CTTGGCCGGA CCACGGAGTG CCTGAATATC CCACCCCCAT CCTAGCCTTC 
CTGCGCAGGG TCAAAGCCTG CAACCCTCCT GATGCTGGAC CCATGGTAGT GCACTGCAGT 
GCTGGAGTTG GCCGTACAGG CTGCTTCATA GTCATCGATG CGATGTTGGA GCGCATGAAG 
CACGAGAAGA CCGTAGACAT TTATGGCCAC GTCACGTGCA TGAGATCCCA GAGGAACTAT 
ATGGTGCAGA CGGAGGACCA GTACATCTTT ATCCACGAGG CCCTTCTGGA GGCCGCCACC 
TGTGGAAACA CAGAGGTCCC TGCCCGAAAC CTCTACGCCC ACATCCAGAA GCTCACACAG 
CCATCGGCTG GAGAGACCGT CACAGCCATG GAGCTGGAGT TTAAGAGACT CGCCAACTCC 
AAAGCCCACA CCTCAAGATT CATCAGCGCC AACCTGCCCT GCAACAAGTT CAAGAACCGG 
CTGGTCAATA TCATGCCTTT CGAGTCCACA CGGGTGTGCC TGCAGCCAAT CCGAGGAGTG 
GAGGGGTCCG ACTACATCAA TGCCAGCTGC ATTGACGGAT ACAGACAGCA GAAGGCCTAT 
ATTGCCACTC AGGGTCCTCT AGCGGAGACC ACCGAGGACT TCTGGAGGAT GTTATGGGAG 
CACAATTCCA CTATTGTAGT GATGCTGACC AAACTCAGAG AGATGGGACG GGAGAAGTGT 
CATCAGTACT GGCCCGCCGA GCGCTCAGCC CGCTATCAGT ACTTCGTAGT GGATCCCATG 
GCCGAATACA ACATGCCCCA GTACATCCTG CGAGAGTTCA AAGTCACAGA TGCCAGGGAC 
GGTCAATCCA GAACCATTCG CCAGTTCCAG TTCACTGATT GGCCAGAGCA GGGAGTGCCA 
AAAACCGGTG AGGGGTTCAT CGATTTCATT GGGCAAGTTC ATAAAACCAA GGAGCAGTTT 
GGGCAGGATG GACCAATCAC TGTGCACTGC AGTGCTGGAG TGGGAAGAAC AGGTGTTTTC 
ATCACTCTCA GTATTGTCCT GGAGAGGATG AGATATGAGG GGGTGGTGGA TCTGTTCCAG 
ACGGTTAAAA CCCTGCGCAC ACAAAGGCCT GCCATGGTGC AGACAGAGGA CCAGTACCAG 
CTGTGCTACA GAGCCGCTCT GGAGTACCTG GGGAGCTTTG ATCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolutionary recruitment of flexible Esrp-dependent splicing programs into diverse embryonic morphogenetic processes.
Nature communications8(1)1799(2017 Nov)
Burguera D,Marquez Y,Racioppi C,Permanyer J,Torres-M?ndez A,Esposito R,Albuixech-Crespo B,Fanlo L,D'Agostino Y,Gohr A,Navas-Perez E,Riesgo A,Cuomo C,Benvenuto G,Christiaen LA,Mart? E,D'Aniello S,Spagnuolo A,Ristoratore F,Arnone MI,Garcia-Fern?ndez J,Irimia M

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Integrative view of ?2,3-sialyltransferases (ST3Gal) molecular and functional evolution in deuterostomes: significance of lineage-specific losses.
Molecular biology and evolution32(4)906-27(2015 Apr)
Petit D,Teppa E,Mir AM,Vicogne D,Thisse C,Thisse B,Filloux C,Harduin-Lepers A

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ptprfb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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