PTPRF cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PTPRF
Entrez Gene ID	512072
Full Name	protein tyrosine phosphatase, receptor type F
General protein information	Preferred Names protein tyrosine phosphatase, receptor type F Names receptor-type tyrosine-protein phosphatase F
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024987593.1	XP_024843361.1	receptor-type tyrosine-protein phosphatase F isoform X7
XM_024987577.1	XP_024843345.1	receptor-type tyrosine-protein phosphatase F isoform X2
XM_024987579.1	XP_024843347.1	receptor-type tyrosine-protein phosphatase F isoform X3
XM_024987581.1	XP_024843349.1	receptor-type tyrosine-protein phosphatase F isoform X4
XM_024987584.1	XP_024843352.1	receptor-type tyrosine-protein phosphatase F isoform X5
XM_024987574.1	XP_024843342.1	receptor-type tyrosine-protein phosphatase F isoform X1
XM_024987591.1	XP_024843359.1	receptor-type tyrosine-protein phosphatase F isoform X6
NM_001101079.1	NP_001094549.1	receptor-type tyrosine-protein phosphatase F precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ptprf	NP_062122.1
Danio rerio (zebrafish)	ptprfb	NP_001077045.1
Danio rerio (zebrafish)	ptprfa	XP_692206.6
	PTPRF	XP_001095521.2
Pan troglodytes (chimpanzee)	PTPRF	XP_001173898.2
Canis lupus familiaris (dog)	PTPRF	XP_005629167.1
Gallus gallus (chicken)	PTPRF	XP_004936750.1
Homo sapiens (human)	PTPRF	NP_002831.2
Mus musculus (house mouse)	Ptprf	NP_035343.2
Bos taurus (cattle)	PTPRF	NP_001094549.1
Xenopus tropicalis (tropical clawed frog)	ptprf	XP_004914019.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PTPRF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTPRF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa232660	XM_024987574.1 Latest version!	Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa232661	XM_024987577.1 Latest version!	Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa232662	XM_024987579.1 Latest version!	Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa232663	XM_024987581.1 Latest version!	Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa232664	XM_024987584.1 Latest version!	Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa232665	XM_024987591.1 Latest version!	Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa232666	XM_024987593.1 Latest version!	Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa44324	NM_001101079.1 Latest version!	Bos taurus protein tyrosine phosphatase receptor type F (PTPRF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PTPRF ( XM_024987574.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843342.1 Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa232660
Clone ID Related Accession (Same CDS sequence)	XM_024987574.1
Accession Version	XM_024987574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCTG AGCCAGCCCC AGGGAGGACG ATGGTGCCCC TTGTCCCTGC ACTTGTGATG 
CTTGGTTTGG TGGCAGGTGC CCATGGCGAC AGCAAACCTG TCTTTGTCAA GGTCCCCGAG 
GACCAGACTG GATTGTCAGG AGGGGTGGCC TCCTTCGTGT GCCAAGCCAC GGGGGAACCC 
AAGCCGCGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTCGAGGTC 
ATCGAGTTTG ATGACGGGGC AGGGTCTGTG CTGCGGATCC AGCCACTGCG GGTGCAGCGA 
GACGAAGCCA TCTATGAGTG CACGGCCACC AACAGTCTGG GGGAGATCAA CACCAGTGCC 
AAGCTCTCAG TGCTCGAAGA GGAACAGCTG CCCCTTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCCCGC ACGGCCACTA TGCTGTGTGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC CGCCGCGAGC 
AACGGCCGCA TCAAGCAACT GCGTTCAGGT GCCTTGCAGA TTGAGAGCAG CGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCTGCAG GCACTCGTTA CTCGGCCCCT 
GCCAACCTAT ATGTGCGAGT GCGCCGCGTG GCTCCGCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGCGG CAGCGTGAAC CTGACGTGCG TCGCCGTTGG CGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAA GAGCTCACCA AGGAGGACGA GATGCCGGTC 
GGCCGCAATG TGCTGGAGCT CAGCAACGTG GTGCGCTCCG CCAACTACAC TTGCGTGGCC 
ATCTCCTCCC TGGGCATGAT CGAGGCCACG GCCCAGGTCA CCGTGAAAGC CCTGCCGAAG 
CCTCCAATTG ACCTTGTGGT GACAGAGACA ACTGCCACCA GCGTCACTCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT ATCCTACTAT GGCATCCAGT ACCGCCCAGC GGGCGCGGAG 
GGCCCCTTCC AGGAGGTGGA CGGCGTGGCC ACCACCCGCT ACAGCATCGG CGGCCTGAGC 
CCTTTCTCAG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGGCG CGGGCCGCCC 
AGCGAGGCGG TGCGGGCGCG CACGGGGGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTAAGCGC CAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCT 
AACGGCCTGG TGCGGGGCTA CCGCGTCTAC TACACCCCCG ACTCCCGCCG CCCTCTGAGC 
GCCTGGCACA AGCACAACAC AGACGCCGGG CTCCTCACCA CTGTGGGCAG CCTGCTGCCT 
GGCATCACCT ACAGCCTGCG TGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCCCCCAGC 
CCCACCATCC AGGTCAAGAC GCAGCAGGGA GTGCCTGCCC AGCCCGCGGA CTTCCAGGCC 
GAGGTGGACT CAGACACCAG GATCCAGCTC TCGTGGCTGC TGCCCCCGCA GGAGCGGATC 
GTCAAGTATG AGCTGGTGTA CTGGGCGGCA GAGGACGAGG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CGTCCTCCTA TACCGTAGAG GACCTGAAGC CCGACACACT GTACCGCTTC 
CAGCTGGCCG CCCGCTCTGA GCTGGGGGTG GGAGTCTTCA CCCCCACCAT TGAGGCTCGC 
ACAGCACAGT CCACCCCCTC CGCACCCCCC CAGAAGGTGA CGTGTGTGAG CGTGGGCTCC 
ACCACCGTCC GGGTAAGTTG GGTCCCGCCG CCGGCCGACA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCGGTGGAC GGCGAGGACC GTGGGCGGCA CGTGGTGGAC 
GGCATCGGCC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACGGACGTG GGCCCCGGCC CCGAGAGCAG CCCGGTGCTG 
GTCCGCACCG ACGAGGACGT GCCCAGTGGG CCACCGCGGA AGGTGGAGGT GGAGCCGCTC 
AACTCCACTG CCGTGCGCGT CTCCTGGAAG CTGCCCGTCC CCAGCAAGCA GCATGGCCAG 
ATCCGCGGCT ACCAGGTCAC CTACGTGCGG CTGGAGAATG GCGAGCCCCG CGGCGCCCCC 
ATCATCCAGG ACGTCATGCT GGCCGAGGCC CAGTGGCAGC CAGAGGAGTC CGAGGACTAT 
GAAACCACCA TCAGCGGCCT GACCCCGGAG ACCACCTACT CCATCACCGT GGCTGCCTAC 
ACCACCAAGG GGGACGGTGC CCGCAGCAAG CCCAAAATTG TCACGACCAC GGGGGCAGTC 
CCAGGACGGC CCACCATGAT GGTCAGCACC ACGGCCATGA ACACGGCGCT GCTCCAGTGG 
CACCCGCCCA AGGAGCTGCC CGGGGAGCTC CTGGGCTACC GGCTGCAGTA CCGCCGAGCC 
GACGAGGCGC GGCCCAGCAC CATCGATTTT GGCAAGGACG ACCAGCACTT TACGGTCACT 
GGCCTGCACA AAGGGGCCAC CTACATCTTC CGGCTCACTG CCAAGAACCG GGCCGGCCTG 
GGCGAGGAGT TCGAGAAGGA AATCACGACC CCCGAGGACG TGCCCAGCGG TTTCCCCCAA 
AACCTGCGCG TGATAGGGCT GACCACATCT ACCACGGAAC TGATCTGGGA CCCCCCAGTG 
CTGGCGGAGA GGAACGGGCG CATCACCAAC TACACCGTGG TGTACCGTGA CATCAACAGC 
CAGCAGGAGC TTCAGAACGT CACTGCCGAC ACCCACCTGA CGCTCTCCGG CCTCAAGCCA 
GACACCACTT ACGACATCAA GGTCCGCGCA CGCACCAGCA AGGGCGCCGG CCCGCTCAGC 
CCCAGCATCC AGTCCCGGAC CATGCCCGTG GAGCAAGTGT TTGCCAAGAA CTTCCGTGTG 
GAGGCTGCAA TGAAGACATC TGTGCTGCTC AGCTGGGAGG TGCCCGACTC CTACAAGTCA 
GCAGTGCCCT TCAGGATCCT GTACAATGGG CAGAGCGTGG AGGTGGACGG GCACTCCATG 
CGGAAGCTGA TTGCAGACCT GCAGCCCAAC ACCGAATACT CATTTGTGCT GATGAACCGC 
GGCAGCAGCG CGGGGGGCCT GCAACACCTC GTGTCCATCC GCACGGCCCC CGACCTCCTG 
CCGCACAAGC CACTGCCAGC CTCGGCCTAC ATCGAGGACG GCCGCTTCAC CCTCACCATG 
CCCCGCGTGC AGGAACCCGC ACTGGTCAGG TGGTTCTACA TCATGGTGGT GCCCATTGAC 
CGCATGGGAG GGAGCATGCT GGCGCCACAG TGGAGCACGC CCGAGGAGCT GGAGCTGGAC 
GAGCTGCTGG AGGCCATCGA GCAGGGCGGC GGGGAGCGGC TGCGGCGCCG GCGGCAGACG 
GAGCGGCTGA AGCCATACGT GGCCGCTCAG GTGGACGTTC TCCCGGAGAC CTTCACCTTG 
GGGGACAAGA AGAACTATCA AGGCTTCTAC AACCGGCCGC TGTCTCCAGA CCTGAGCTAC 
CAGTGCTTTG TGCTCGCCTC CCTGAAGGAA CCCGTGGACC AGAAGCGCTA TGCCTGCAGC 
CCCTACTCCG ATGAGATCGT GGTCCAGGTG ACACCAGCCC AGCAGCAGGA GGAACCCGAG 
CTGCTGTGGG TGACGGGCCC CGTCCTGGCA GTCATTCTCA TCGTCCTCAT TGTCATCGCC 
ATTCTCCTGT TCAAGAGGAA AAGGACCCAC TCCCCATCGT CCAAGGATGA GCAGTCAATT 
GGGCTGAAGG ACTCCTTGCT GGCCCACTCC TCCGACCCTG TGGAGATGCG GCGGCTCAAC 
TACCAGACCC CAGGTATGCG AGACCACCCG CCCATCCCCA TCACCGACCT AGCTGACAAC 
ATCGAGCGCC TCAAAGCCAA TGACGGCCTC AAGTTCTCCC AGGAGTACGA GTCAATTGAC 
CCAGGACAGC AGTTCACGTG GGAGAATTCA AACCTGGAGG TGAACAAGCC GAAGAACCGC 
TATGCCAACG TCATTGCTTA CGACCACTCT CGAGTCATCC TTACCTCCAT CGATGGTGTC 
CCCGGGAGCG ACTACATCAA TGCCAATTAC ATCGACGGCT ACCGCAAGCA GAACGCCTAC 
ATTGCCACGC AGGGCCCGCT GCCTGAGACC ATGGGCGATT TCTGGCGGAT GGTGTGGGAA 
CAGCGCACAG CCACTGTGGT CATGATGACG CGGCTGGAGG AGAAGTCCCG GGTGAAATGT 
GACCAGTACT GGCCAGCTCG TGGCACCGAG ACCTATGGCC TCATCCAGGT GACCCTGCTG 
GACACGGTGG AGCTGGCCAC CTACACTGTG CGCACCTTCG CGCTCTACAA GAGTGGCTCC 
AGTGAGAAGC GGGAGCTGCG TCAGTTCCAG TTCATGGCCT GGCCAGACCA CGGAGTCCCC 
GAGTACCCGA CCCCTATCCT GGCCTTCCTG CGGCGGGTCA AGGCTTGCAA CCCCCTGGAC 
GCGGGGCCCA TGGTGGTGCA CTGCAGCGCG GGCGTGGGCC GCACGGGCTG CTTCATCGTC 
ATCGACGCCA TGCTGGAGCG GATGAAGCAC GAGAAGACAG TGGACATCTA CGGCCACGTG 
ACGTGCATGC GGGCGCAGCG CAACTACATG GTGCAGACTG AGGACCAGTA CGTGTTCATC 
CACGAGGCGC TGCTGGAGGC CGCCATGTGT GGACACACGG AGGTGCCCGC CCGCAACCTC 
TACGCCCACA TCCAGAAGCT GGGCCAAGTG CCTCCCGGGG AGAGTGTGAC TGCCATGGAG 
CTGGAGTTCA AGTTGCTGGC CAACTCCAAG GCCCACACAT CCCGCTTCAT CAGCGCCAAC 
CTGCCCTGCA ACAAGTTCAA GAACCGCCTG GTGAACATCA TGCCCTACGA ACTGACCCGT 
GTGTGTCTGC AGCCCATCCG CGGCGTGGAG GGCTCCGACT ACATCAATGC CAGCTTCCTG 
GATGGCTATC GACAGCAGAA GGCCTACATA GCCACACAGG GGCCTCTGGC GGAGAGCACC 
GAGGATTTCT GGCGCATGTT ATGGGAGCAC AACTCCACCA TCATCGTCAT GCTGACCAGG 
CTTCGGGAGA TGGGCCGGGA GAAATGCCAC CAGTACTGGC CTGCAGAGCG CTCTGCTCGC 
TACCAGTACT TTGTCGTCGA CCCGATGGCT GAGTACAACA TGCCCCAGTA TATTCTCCGT 
GAGTTCAAGG TCACGGATGC CCGGGATGGG CAGTCGAGGA CAATCCGGCA GTTCCAGTTC 
ACAGACTGGC CGGAGCAGGG CGTGCCTAAA ACGGGCGAGG GCTTCATCGA CTTCATCGGG 
CAGGTGCACA AGACCAAGGA GCAGTTCGGG CAGGACGGGC CCATCACGGT GCACTGCAGT 
GCTGGTGTGG GCCGCACTGG GGTGTTCATC ACGCTGAGCA TTGTCCTGGA GCGGATGCGC 
TACGAAGGCG TGGTTGACAT GTTCCAGACC GTGAAGACCC TGCGCACACA GCGCCCAGCC 
ATGGTGCAGA CGGAGGACCA GTATCAGCTG TGCTACCGCG CGGCCCTGGA GTACCTCGGA 
AGCTTTGACC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843342.1
CDS	292..6015
Translation	MTPEPAPGRTMVPLVPALVMLGLVAGAHGDSKPVFVKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPLGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPAASNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLVVTETTATSVTLTWDSGNSEPVSYYGIQYRPAGAEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDSRRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEVDSDTRIQLSWLLPPQERIVKYELVYWAAEDEGQQHKVTFDPTSSYTVEDLKPDTLYRFQLAARSELGVGVFTPTIEARTAQSTPSAPPQKVTCVSVGSTTVRVSWVPPPADSRNGVITQYSVAYEAVDGEDRGRHVVDGIGREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGAPIIQDVMLAEAQWQPEESEDYETTISGLTPETTYSITVAAYTTKGDGARSKPKIVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYRRADEARPSTIDFGKDDQHFTVTGLHKGATYIFRLTAKNRAGLGEEFEKEITTPEDVPSGFPQNLRVIGLTTSTTELIWDPPVLAERNGRITNYTVVYRDINSQQELQNVTADTHLTLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPVEQVFAKNFRVEAAMKTSVLLSWEVPDSYKSAVPFRILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPLPASAYIEDGRFTLTMPRVQEPALVRWFYIMVVPIDRMGGSMLAPQWSTPEELELDELLEAIEQGGGERLRRRRQTERLKPYVAAQVDVLPETFTLGDKKNYQGFYNRPLSPDLSYQCFVLASLKEPVDQKRYACSPYSDEIVVQVTPAQQQEEPELLWVTGPVLAVILIVLIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSIDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETYGLIQVTLLDTVELATYTVRTFALYKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRAQRNYMVQTEDQYVFIHEALLEAAMCGHTEVPARNLYAHIQKLGQVPPGESVTAMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHNSTIIVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_024987574.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987574.1

ORF Insert Sequence:

ATGACCCCTG AGCCAGCCCC AGGGAGGACG ATGGTGCCCC TTGTCCCTGC ACTTGTGATG 
CTTGGTTTGG TGGCAGGTGC CCATGGCGAC AGCAAACCTG TCTTTGTCAA GGTCCCCGAG 
GACCAGACTG GATTGTCAGG AGGGGTGGCC TCCTTCGTGT GCCAAGCCAC GGGGGAACCC 
AAGCCGCGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTCGAGGTC 
ATCGAGTTTG ATGACGGGGC AGGGTCTGTG CTGCGGATCC AGCCACTGCG GGTGCAGCGA 
GACGAAGCCA TCTATGAGTG CACGGCCACC AACAGTCTGG GGGAGATCAA CACCAGTGCC 
AAGCTCTCAG TGCTCGAAGA GGAACAGCTG CCCCTTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCCCGC ACGGCCACTA TGCTGTGTGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC CGCCGCGAGC 
AACGGCCGCA TCAAGCAACT GCGTTCAGGT GCCTTGCAGA TTGAGAGCAG CGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCTGCAG GCACTCGTTA CTCGGCCCCT 
GCCAACCTAT ATGTGCGAGT GCGCCGCGTG GCTCCGCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGCGG CAGCGTGAAC CTGACGTGCG TCGCCGTTGG CGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAA GAGCTCACCA AGGAGGACGA GATGCCGGTC 
GGCCGCAATG TGCTGGAGCT CAGCAACGTG GTGCGCTCCG CCAACTACAC TTGCGTGGCC 
ATCTCCTCCC TGGGCATGAT CGAGGCCACG GCCCAGGTCA CCGTGAAAGC CCTGCCGAAG 
CCTCCAATTG ACCTTGTGGT GACAGAGACA ACTGCCACCA GCGTCACTCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT ATCCTACTAT GGCATCCAGT ACCGCCCAGC GGGCGCGGAG 
GGCCCCTTCC AGGAGGTGGA CGGCGTGGCC ACCACCCGCT ACAGCATCGG CGGCCTGAGC 
CCTTTCTCAG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGGCG CGGGCCGCCC 
AGCGAGGCGG TGCGGGCGCG CACGGGGGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTAAGCGC CAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCT 
AACGGCCTGG TGCGGGGCTA CCGCGTCTAC TACACCCCCG ACTCCCGCCG CCCTCTGAGC 
GCCTGGCACA AGCACAACAC AGACGCCGGG CTCCTCACCA CTGTGGGCAG CCTGCTGCCT 
GGCATCACCT ACAGCCTGCG TGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCCCCCAGC 
CCCACCATCC AGGTCAAGAC GCAGCAGGGA GTGCCTGCCC AGCCCGCGGA CTTCCAGGCC 
GAGGTGGACT CAGACACCAG GATCCAGCTC TCGTGGCTGC TGCCCCCGCA GGAGCGGATC 
GTCAAGTATG AGCTGGTGTA CTGGGCGGCA GAGGACGAGG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CGTCCTCCTA TACCGTAGAG GACCTGAAGC CCGACACACT GTACCGCTTC 
CAGCTGGCCG CCCGCTCTGA GCTGGGGGTG GGAGTCTTCA CCCCCACCAT TGAGGCTCGC 
ACAGCACAGT CCACCCCCTC CGCACCCCCC CAGAAGGTGA CGTGTGTGAG CGTGGGCTCC 
ACCACCGTCC GGGTAAGTTG GGTCCCGCCG CCGGCCGACA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCGGTGGAC GGCGAGGACC GTGGGCGGCA CGTGGTGGAC 
GGCATCGGCC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACGGACGTG GGCCCCGGCC CCGAGAGCAG CCCGGTGCTG 
GTCCGCACCG ACGAGGACGT GCCCAGTGGG CCACCGCGGA AGGTGGAGGT GGAGCCGCTC 
AACTCCACTG CCGTGCGCGT CTCCTGGAAG CTGCCCGTCC CCAGCAAGCA GCATGGCCAG 
ATCCGCGGCT ACCAGGTCAC CTACGTGCGG CTGGAGAATG GCGAGCCCCG CGGCGCCCCC 
ATCATCCAGG ACGTCATGCT GGCCGAGGCC CAGTGGCAGC CAGAGGAGTC CGAGGACTAT 
GAAACCACCA TCAGCGGCCT GACCCCGGAG ACCACCTACT CCATCACCGT GGCTGCCTAC 
ACCACCAAGG GGGACGGTGC CCGCAGCAAG CCCAAAATTG TCACGACCAC GGGGGCAGTC 
CCAGGACGGC CCACCATGAT GGTCAGCACC ACGGCCATGA ACACGGCGCT GCTCCAGTGG 
CACCCGCCCA AGGAGCTGCC CGGGGAGCTC CTGGGCTACC GGCTGCAGTA CCGCCGAGCC 
GACGAGGCGC GGCCCAGCAC CATCGATTTT GGCAAGGACG ACCAGCACTT TACGGTCACT 
GGCCTGCACA AAGGGGCCAC CTACATCTTC CGGCTCACTG CCAAGAACCG GGCCGGCCTG 
GGCGAGGAGT TCGAGAAGGA AATCACGACC CCCGAGGACG TGCCCAGCGG TTTCCCCCAA 
AACCTGCGCG TGATAGGGCT GACCACATCT ACCACGGAAC TGATCTGGGA CCCCCCAGTG 
CTGGCGGAGA GGAACGGGCG CATCACCAAC TACACCGTGG TGTACCGTGA CATCAACAGC 
CAGCAGGAGC TTCAGAACGT CACTGCCGAC ACCCACCTGA CGCTCTCCGG CCTCAAGCCA 
GACACCACTT ACGACATCAA GGTCCGCGCA CGCACCAGCA AGGGCGCCGG CCCGCTCAGC 
CCCAGCATCC AGTCCCGGAC CATGCCCGTG GAGCAAGTGT TTGCCAAGAA CTTCCGTGTG 
GAGGCTGCAA TGAAGACATC TGTGCTGCTC AGCTGGGAGG TGCCCGACTC CTACAAGTCA 
GCAGTGCCCT TCAGGATCCT GTACAATGGG CAGAGCGTGG AGGTGGACGG GCACTCCATG 
CGGAAGCTGA TTGCAGACCT GCAGCCCAAC ACCGAATACT CATTTGTGCT GATGAACCGC 
GGCAGCAGCG CGGGGGGCCT GCAACACCTC GTGTCCATCC GCACGGCCCC CGACCTCCTG 
CCGCACAAGC CACTGCCAGC CTCGGCCTAC ATCGAGGACG GCCGCTTCAC CCTCACCATG 
CCCCGCGTGC AGGAACCCGC ACTGGTCAGG TGGTTCTACA TCATGGTGGT GCCCATTGAC 
CGCATGGGAG GGAGCATGCT GGCGCCACAG TGGAGCACGC CCGAGGAGCT GGAGCTGGAC 
GAGCTGCTGG AGGCCATCGA GCAGGGCGGC GGGGAGCGGC TGCGGCGCCG GCGGCAGACG 
GAGCGGCTGA AGCCATACGT GGCCGCTCAG GTGGACGTTC TCCCGGAGAC CTTCACCTTG 
GGGGACAAGA AGAACTATCA AGGCTTCTAC AACCGGCCGC TGTCTCCAGA CCTGAGCTAC 
CAGTGCTTTG TGCTCGCCTC CCTGAAGGAA CCCGTGGACC AGAAGCGCTA TGCCTGCAGC 
CCCTACTCCG ATGAGATCGT GGTCCAGGTG ACACCAGCCC AGCAGCAGGA GGAACCCGAG 
CTGCTGTGGG TGACGGGCCC CGTCCTGGCA GTCATTCTCA TCGTCCTCAT TGTCATCGCC 
ATTCTCCTGT TCAAGAGGAA AAGGACCCAC TCCCCATCGT CCAAGGATGA GCAGTCAATT 
GGGCTGAAGG ACTCCTTGCT GGCCCACTCC TCCGACCCTG TGGAGATGCG GCGGCTCAAC 
TACCAGACCC CAGGTATGCG AGACCACCCG CCCATCCCCA TCACCGACCT AGCTGACAAC 
ATCGAGCGCC TCAAAGCCAA TGACGGCCTC AAGTTCTCCC AGGAGTACGA GTCAATTGAC 
CCAGGACAGC AGTTCACGTG GGAGAATTCA AACCTGGAGG TGAACAAGCC GAAGAACCGC 
TATGCCAACG TCATTGCTTA CGACCACTCT CGAGTCATCC TTACCTCCAT CGATGGTGTC 
CCCGGGAGCG ACTACATCAA TGCCAATTAC ATCGACGGCT ACCGCAAGCA GAACGCCTAC 
ATTGCCACGC AGGGCCCGCT GCCTGAGACC ATGGGCGATT TCTGGCGGAT GGTGTGGGAA 
CAGCGCACAG CCACTGTGGT CATGATGACG CGGCTGGAGG AGAAGTCCCG GGTGAAATGT 
GACCAGTACT GGCCAGCTCG TGGCACCGAG ACCTATGGCC TCATCCAGGT GACCCTGCTG 
GACACGGTGG AGCTGGCCAC CTACACTGTG CGCACCTTCG CGCTCTACAA GAGTGGCTCC 
AGTGAGAAGC GGGAGCTGCG TCAGTTCCAG TTCATGGCCT GGCCAGACCA CGGAGTCCCC 
GAGTACCCGA CCCCTATCCT GGCCTTCCTG CGGCGGGTCA AGGCTTGCAA CCCCCTGGAC 
GCGGGGCCCA TGGTGGTGCA CTGCAGCGCG GGCGTGGGCC GCACGGGCTG CTTCATCGTC 
ATCGACGCCA TGCTGGAGCG GATGAAGCAC GAGAAGACAG TGGACATCTA CGGCCACGTG 
ACGTGCATGC GGGCGCAGCG CAACTACATG GTGCAGACTG AGGACCAGTA CGTGTTCATC 
CACGAGGCGC TGCTGGAGGC CGCCATGTGT GGACACACGG AGGTGCCCGC CCGCAACCTC 
TACGCCCACA TCCAGAAGCT GGGCCAAGTG CCTCCCGGGG AGAGTGTGAC TGCCATGGAG 
CTGGAGTTCA AGTTGCTGGC CAACTCCAAG GCCCACACAT CCCGCTTCAT CAGCGCCAAC 
CTGCCCTGCA ACAAGTTCAA GAACCGCCTG GTGAACATCA TGCCCTACGA ACTGACCCGT 
GTGTGTCTGC AGCCCATCCG CGGCGTGGAG GGCTCCGACT ACATCAATGC CAGCTTCCTG 
GATGGCTATC GACAGCAGAA GGCCTACATA GCCACACAGG GGCCTCTGGC GGAGAGCACC 
GAGGATTTCT GGCGCATGTT ATGGGAGCAC AACTCCACCA TCATCGTCAT GCTGACCAGG 
CTTCGGGAGA TGGGCCGGGA GAAATGCCAC CAGTACTGGC CTGCAGAGCG CTCTGCTCGC 
TACCAGTACT TTGTCGTCGA CCCGATGGCT GAGTACAACA TGCCCCAGTA TATTCTCCGT 
GAGTTCAAGG TCACGGATGC CCGGGATGGG CAGTCGAGGA CAATCCGGCA GTTCCAGTTC 
ACAGACTGGC CGGAGCAGGG CGTGCCTAAA ACGGGCGAGG GCTTCATCGA CTTCATCGGG 
CAGGTGCACA AGACCAAGGA GCAGTTCGGG CAGGACGGGC CCATCACGGT GCACTGCAGT 
GCTGGTGTGG GCCGCACTGG GGTGTTCATC ACGCTGAGCA TTGTCCTGGA GCGGATGCGC 
TACGAAGGCG TGGTTGACAT GTTCCAGACC GTGAAGACCC TGCGCACACA GCGCCCAGCC 
ATGGTGCAGA CGGAGGACCA GTATCAGCTG TGCTACCGCG CGGCCCTGGA GTACCTCGGA 
AGCTTTGACC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_024987577.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843345.1 Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X2, mRNA.

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CloneID	OBa232661
Clone ID Related Accession (Same CDS sequence)	XM_024987577.1
Accession Version	XM_024987577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	receptor-type tyrosine-protein phosphatase F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCTG AGCCAGCCCC AGGGAGGACG ATGGTGCCCC TTGTCCCTGC ACTTGTGATG 
CTTGGTTTGG TGGCAGGTGC CCATGGCGAC AGCAAACCTG TCTTTGTCAA GGTCCCCGAG 
GACCAGACTG GATTGTCAGG AGGGGTGGCC TCCTTCGTGT GCCAAGCCAC GGGGGAACCC 
AAGCCGCGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTCGAGGTC 
ATCGAGTTTG ATGACGGGGC AGGGTCTGTG CTGCGGATCC AGCCACTGCG GGTGCAGCGA 
GACGAAGCCA TCTATGAGTG CACGGCCACC AACAGTCTGG GGGAGATCAA CACCAGTGCC 
AAGCTCTCAG TGCTCGAAGA GGAACAGCTG CCCCTTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCCCGC ACGGCCACTA TGCTGTGTGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC CGCCGCGAGC 
AACGGCCGCA TCAAGCAACT GCGTTCAGGT GCCTTGCAGA TTGAGAGCAG CGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCTGCAG GCACTCGTTA CTCGGCCCCT 
GCCAACCTAT ATGTGCGAGT GCGCCGCGTG GCTCCGCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGCGG CAGCGTGAAC CTGACGTGCG TCGCCGTTGG CGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAA GAGCTCACCA AGGAGGACGA GATGCCGGTC 
GGCCGCAATG TGCTGGAGCT CAGCAACGTG GTGCGCTCCG CCAACTACAC TTGCGTGGCC 
ATCTCCTCCC TGGGCATGAT CGAGGCCACG GCCCAGGTCA CCGTGAAAGC CCTGCCGAAG 
CCTCCAATTG ACCTTGTGGT GACAGAGACA ACTGCCACCA GCGTCACTCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT ATCCTACTAT GGCATCCAGT ACCGCCCAGC GGGCGCGGAG 
GGCCCCTTCC AGGAGGTGGA CGGCGTGGCC ACCACCCGCT ACAGCATCGG CGGCCTGAGC 
CCTTTCTCAG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGGCG CGGGCCGCCC 
AGCGAGGCGG TGCGGGCGCG CACGGGGGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTAAGCGC CAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCT 
AACGGCCTGG TGCGGGGCTA CCGCGTCTAC TACACCCCCG ACTCCCGCCG CCCTCTGAGC 
GCCTGGCACA AGCACAACAC AGACGCCGGG CTCCTCACCA CTGTGGGCAG CCTGCTGCCT 
GGCATCACCT ACAGCCTGCG TGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCCCCCAGC 
CCCACCATCC AGGTCAAGAC GCAGCAGGGA GTGCCTGCCC AGCCCGCGGA CTTCCAGGCC 
GAGGTGGACT CAGACACCAG GATCCAGCTC TCGTGGCTGC TGCCCCCGCA GGAGCGGATC 
GTCAAGTATG AGCTGGTGTA CTGGGCGGCA GAGGACGAGG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CGTCCTCCTA TACCGTAGAG GACCTGAAGC CCGACACACT GTACCGCTTC 
CAGCTGGCCG CCCGCTCTGA GCTGGGGGTG GGAGTCTTCA CCCCCACCAT TGAGGCTCGC 
ACAGCACAGT CCATGCCCAG TGGGCCACCG CGGAAGGTGG AGGTGGAGCC GCTCAACTCC 
ACTGCCGTGC GCGTCTCCTG GAAGCTGCCC GTCCCCAGCA AGCAGCATGG CCAGATCCGC 
GGCTACCAGG TCACCTACGT GCGGCTGGAG AATGGCGAGC CCCGCGGCGC CCCCATCATC 
CAGGACGTCA TGCTGGCCGA GGCCCAGTGG CAGCCAGAGG AGTCCGAGGA CTATGAAACC 
ACCATCAGCG GCCTGACCCC GGAGACCACC TACTCCATCA CCGTGGCTGC CTACACCACC 
AAGGGGGACG GTGCCCGCAG CAAGCCCAAA ATTGTCACGA CCACGGGGGC AGTCCCAGGA 
CGGCCCACCA TGATGGTCAG CACCACGGCC ATGAACACGG CGCTGCTCCA GTGGCACCCG 
CCCAAGGAGC TGCCCGGGGA GCTCCTGGGC TACCGGCTGC AGTACCGCCG AGCCGACGAG 
GCGCGGCCCA GCACCATCGA TTTTGGCAAG GACGACCAGC ACTTTACGGT CACTGGCCTG 
CACAAAGGGG CCACCTACAT CTTCCGGCTC ACTGCCAAGA ACCGGGCCGG CCTGGGCGAG 
GAGTTCGAGA AGGAAATCAC GACCCCCGAG GACGTGCCCA GCGGTTTCCC CCAAAACCTG 
CGCGTGATAG GGCTGACCAC ATCTACCACG GAACTGATCT GGGACCCCCC AGTGCTGGCG 
GAGAGGAACG GGCGCATCAC CAACTACACC GTGGTGTACC GTGACATCAA CAGCCAGCAG 
GAGCTTCAGA ACGTCACTGC CGACACCCAC CTGACGCTCT CCGGCCTCAA GCCAGACACC 
ACTTACGACA TCAAGGTCCG CGCACGCACC AGCAAGGGCG CCGGCCCGCT CAGCCCCAGC 
ATCCAGTCCC GGACCATGCC CGTGGAGCAA GTGTTTGCCA AGAACTTCCG TGTGGAGGCT 
GCAATGAAGA CATCTGTGCT GCTCAGCTGG GAGGTGCCCG ACTCCTACAA GTCAGCAGTG 
CCCTTCAGGA TCCTGTACAA TGGGCAGAGC GTGGAGGTGG ACGGGCACTC CATGCGGAAG 
CTGATTGCAG ACCTGCAGCC CAACACCGAA TACTCATTTG TGCTGATGAA CCGCGGCAGC 
AGCGCGGGGG GCCTGCAACA CCTCGTGTCC ATCCGCACGG CCCCCGACCT CCTGCCGCAC 
AAGCCACTGC CAGCCTCGGC CTACATCGAG GACGGCCGCT TCACCCTCAC CATGCCCCGC 
GTGCAGGAAC CCGCACTGGT CAGGTGGTTC TACATCATGG TGGTGCCCAT TGACCGCATG 
GGAGGGAGCA TGCTGGCGCC ACAGTGGAGC ACGCCCGAGG AGCTGGAGCT GGACGAGCTG 
CTGGAGGCCA TCGAGCAGGG CGGCGGGGAG CGGCTGCGGC GCCGGCGGCA GACGGAGCGG 
CTGAAGCCAT ACGTGGCCGC TCAGGTGGAC GTTCTCCCGG AGACCTTCAC CTTGGGGGAC 
AAGAAGAACT ATCAAGGCTT CTACAACCGG CCGCTGTCTC CAGACCTGAG CTACCAGTGC 
TTTGTGCTCG CCTCCCTGAA GGAACCCGTG GACCAGAAGC GCTATGCCTG CAGCCCCTAC 
TCCGATGAGA TCGTGGTCCA GGTGACACCA GCCCAGCAGC AGGAGGAACC CGAGCTGCTG 
TGGGTGACGG GCCCCGTCCT GGCAGTCATT CTCATCGTCC TCATTGTCAT CGCCATTCTC 
CTGTTCAAGA GGAAAAGGAC CCACTCCCCA TCGTCCAAGG ATGAGCAGTC AATTGGGCTG 
AAGGACTCCT TGCTGGCCCA CTCCTCCGAC CCTGTGGAGA TGCGGCGGCT CAACTACCAG 
ACCCCAGGTA TGCGAGACCA CCCGCCCATC CCCATCACCG ACCTAGCTGA CAACATCGAG 
CGCCTCAAAG CCAATGACGG CCTCAAGTTC TCCCAGGAGT ACGAGTCAAT TGACCCAGGA 
CAGCAGTTCA CGTGGGAGAA TTCAAACCTG GAGGTGAACA AGCCGAAGAA CCGCTATGCC 
AACGTCATTG CTTACGACCA CTCTCGAGTC ATCCTTACCT CCATCGATGG TGTCCCCGGG 
AGCGACTACA TCAATGCCAA TTACATCGAC GGCTACCGCA AGCAGAACGC CTACATTGCC 
ACGCAGGGCC CGCTGCCTGA GACCATGGGC GATTTCTGGC GGATGGTGTG GGAACAGCGC 
ACAGCCACTG TGGTCATGAT GACGCGGCTG GAGGAGAAGT CCCGGGTGAA ATGTGACCAG 
TACTGGCCAG CTCGTGGCAC CGAGACCTAT GGCCTCATCC AGGTGACCCT GCTGGACACG 
GTGGAGCTGG CCACCTACAC TGTGCGCACC TTCGCGCTCT ACAAGAGTGG CTCCAGTGAG 
AAGCGGGAGC TGCGTCAGTT CCAGTTCATG GCCTGGCCAG ACCACGGAGT CCCCGAGTAC 
CCGACCCCTA TCCTGGCCTT CCTGCGGCGG GTCAAGGCTT GCAACCCCCT GGACGCGGGG 
CCCATGGTGG TGCACTGCAG CGCGGGCGTG GGCCGCACGG GCTGCTTCAT CGTCATCGAC 
GCCATGCTGG AGCGGATGAA GCACGAGAAG ACAGTGGACA TCTACGGCCA CGTGACGTGC 
ATGCGGGCGC AGCGCAACTA CATGGTGCAG ACTGAGGACC AGTACGTGTT CATCCACGAG 
GCGCTGCTGG AGGCCGCCAT GTGTGGACAC ACGGAGGTGC CCGCCCGCAA CCTCTACGCC 
CACATCCAGA AGCTGGGCCA AGTGCCTCCC GGGGAGAGTG TGACTGCCAT GGAGCTGGAG 
TTCAAGTTGC TGGCCAACTC CAAGGCCCAC ACATCCCGCT TCATCAGCGC CAACCTGCCC 
TGCAACAAGT TCAAGAACCG CCTGGTGAAC ATCATGCCCT ACGAACTGAC CCGTGTGTGT 
CTGCAGCCCA TCCGCGGCGT GGAGGGCTCC GACTACATCA ATGCCAGCTT CCTGGATGGC 
TATCGACAGC AGAAGGCCTA CATAGCCACA CAGGGGCCTC TGGCGGAGAG CACCGAGGAT 
TTCTGGCGCA TGTTATGGGA GCACAACTCC ACCATCATCG TCATGCTGAC CAGGCTTCGG 
GAGATGGGCC GGGAGAAATG CCACCAGTAC TGGCCTGCAG AGCGCTCTGC TCGCTACCAG 
TACTTTGTCG TCGACCCGAT GGCTGAGTAC AACATGCCCC AGTATATTCT CCGTGAGTTC 
AAGGTCACGG ATGCCCGGGA TGGGCAGTCG AGGACAATCC GGCAGTTCCA GTTCACAGAC 
TGGCCGGAGC AGGGCGTGCC TAAAACGGGC GAGGGCTTCA TCGACTTCAT CGGGCAGGTG 
CACAAGACCA AGGAGCAGTT CGGGCAGGAC GGGCCCATCA CGGTGCACTG CAGTGCTGGT 
GTGGGCCGCA CTGGGGTGTT CATCACGCTG AGCATTGTCC TGGAGCGGAT GCGCTACGAA 
GGCGTGGTTG ACATGTTCCA GACCGTGAAG ACCCTGCGCA CACAGCGCCC AGCCATGGTG 
CAGACGGAGG ACCAGTATCA GCTGTGCTAC CGCGCGGCCC TGGAGTACCT CGGAAGCTTT 
GACCACTATG CAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843345.1
CDS	291..5708
Translation	MTPEPAPGRTMVPLVPALVMLGLVAGAHGDSKPVFVKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPLGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPAASNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLVVTETTATSVTLTWDSGNSEPVSYYGIQYRPAGAEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDSRRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEVDSDTRIQLSWLLPPQERIVKYELVYWAAEDEGQQHKVTFDPTSSYTVEDLKPDTLYRFQLAARSELGVGVFTPTIEARTAQSMPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGAPIIQDVMLAEAQWQPEESEDYETTISGLTPETTYSITVAAYTTKGDGARSKPKIVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYRRADEARPSTIDFGKDDQHFTVTGLHKGATYIFRLTAKNRAGLGEEFEKEITTPEDVPSGFPQNLRVIGLTTSTTELIWDPPVLAERNGRITNYTVVYRDINSQQELQNVTADTHLTLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPVEQVFAKNFRVEAAMKTSVLLSWEVPDSYKSAVPFRILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPLPASAYIEDGRFTLTMPRVQEPALVRWFYIMVVPIDRMGGSMLAPQWSTPEELELDELLEAIEQGGGERLRRRRQTERLKPYVAAQVDVLPETFTLGDKKNYQGFYNRPLSPDLSYQCFVLASLKEPVDQKRYACSPYSDEIVVQVTPAQQQEEPELLWVTGPVLAVILIVLIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSIDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETYGLIQVTLLDTVELATYTVRTFALYKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRAQRNYMVQTEDQYVFIHEALLEAAMCGHTEVPARNLYAHIQKLGQVPPGESVTAMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHNSTIIVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_024987577.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987577.1

ORF Insert Sequence:

ATGACCCCTG AGCCAGCCCC AGGGAGGACG ATGGTGCCCC TTGTCCCTGC ACTTGTGATG 
CTTGGTTTGG TGGCAGGTGC CCATGGCGAC AGCAAACCTG TCTTTGTCAA GGTCCCCGAG 
GACCAGACTG GATTGTCAGG AGGGGTGGCC TCCTTCGTGT GCCAAGCCAC GGGGGAACCC 
AAGCCGCGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTCGAGGTC 
ATCGAGTTTG ATGACGGGGC AGGGTCTGTG CTGCGGATCC AGCCACTGCG GGTGCAGCGA 
GACGAAGCCA TCTATGAGTG CACGGCCACC AACAGTCTGG GGGAGATCAA CACCAGTGCC 
AAGCTCTCAG TGCTCGAAGA GGAACAGCTG CCCCTTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCCCGC ACGGCCACTA TGCTGTGTGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC CGCCGCGAGC 
AACGGCCGCA TCAAGCAACT GCGTTCAGGT GCCTTGCAGA TTGAGAGCAG CGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCTGCAG GCACTCGTTA CTCGGCCCCT 
GCCAACCTAT ATGTGCGAGT GCGCCGCGTG GCTCCGCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGCGG CAGCGTGAAC CTGACGTGCG TCGCCGTTGG CGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAA GAGCTCACCA AGGAGGACGA GATGCCGGTC 
GGCCGCAATG TGCTGGAGCT CAGCAACGTG GTGCGCTCCG CCAACTACAC TTGCGTGGCC 
ATCTCCTCCC TGGGCATGAT CGAGGCCACG GCCCAGGTCA CCGTGAAAGC CCTGCCGAAG 
CCTCCAATTG ACCTTGTGGT GACAGAGACA ACTGCCACCA GCGTCACTCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT ATCCTACTAT GGCATCCAGT ACCGCCCAGC GGGCGCGGAG 
GGCCCCTTCC AGGAGGTGGA CGGCGTGGCC ACCACCCGCT ACAGCATCGG CGGCCTGAGC 
CCTTTCTCAG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGGCG CGGGCCGCCC 
AGCGAGGCGG TGCGGGCGCG CACGGGGGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTAAGCGC CAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCT 
AACGGCCTGG TGCGGGGCTA CCGCGTCTAC TACACCCCCG ACTCCCGCCG CCCTCTGAGC 
GCCTGGCACA AGCACAACAC AGACGCCGGG CTCCTCACCA CTGTGGGCAG CCTGCTGCCT 
GGCATCACCT ACAGCCTGCG TGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCCCCCAGC 
CCCACCATCC AGGTCAAGAC GCAGCAGGGA GTGCCTGCCC AGCCCGCGGA CTTCCAGGCC 
GAGGTGGACT CAGACACCAG GATCCAGCTC TCGTGGCTGC TGCCCCCGCA GGAGCGGATC 
GTCAAGTATG AGCTGGTGTA CTGGGCGGCA GAGGACGAGG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CGTCCTCCTA TACCGTAGAG GACCTGAAGC CCGACACACT GTACCGCTTC 
CAGCTGGCCG CCCGCTCTGA GCTGGGGGTG GGAGTCTTCA CCCCCACCAT TGAGGCTCGC 
ACAGCACAGT CCATGCCCAG TGGGCCACCG CGGAAGGTGG AGGTGGAGCC GCTCAACTCC 
ACTGCCGTGC GCGTCTCCTG GAAGCTGCCC GTCCCCAGCA AGCAGCATGG CCAGATCCGC 
GGCTACCAGG TCACCTACGT GCGGCTGGAG AATGGCGAGC CCCGCGGCGC CCCCATCATC 
CAGGACGTCA TGCTGGCCGA GGCCCAGTGG CAGCCAGAGG AGTCCGAGGA CTATGAAACC 
ACCATCAGCG GCCTGACCCC GGAGACCACC TACTCCATCA CCGTGGCTGC CTACACCACC 
AAGGGGGACG GTGCCCGCAG CAAGCCCAAA ATTGTCACGA CCACGGGGGC AGTCCCAGGA 
CGGCCCACCA TGATGGTCAG CACCACGGCC ATGAACACGG CGCTGCTCCA GTGGCACCCG 
CCCAAGGAGC TGCCCGGGGA GCTCCTGGGC TACCGGCTGC AGTACCGCCG AGCCGACGAG 
GCGCGGCCCA GCACCATCGA TTTTGGCAAG GACGACCAGC ACTTTACGGT CACTGGCCTG 
CACAAAGGGG CCACCTACAT CTTCCGGCTC ACTGCCAAGA ACCGGGCCGG CCTGGGCGAG 
GAGTTCGAGA AGGAAATCAC GACCCCCGAG GACGTGCCCA GCGGTTTCCC CCAAAACCTG 
CGCGTGATAG GGCTGACCAC ATCTACCACG GAACTGATCT GGGACCCCCC AGTGCTGGCG 
GAGAGGAACG GGCGCATCAC CAACTACACC GTGGTGTACC GTGACATCAA CAGCCAGCAG 
GAGCTTCAGA ACGTCACTGC CGACACCCAC CTGACGCTCT CCGGCCTCAA GCCAGACACC 
ACTTACGACA TCAAGGTCCG CGCACGCACC AGCAAGGGCG CCGGCCCGCT CAGCCCCAGC 
ATCCAGTCCC GGACCATGCC CGTGGAGCAA GTGTTTGCCA AGAACTTCCG TGTGGAGGCT 
GCAATGAAGA CATCTGTGCT GCTCAGCTGG GAGGTGCCCG ACTCCTACAA GTCAGCAGTG 
CCCTTCAGGA TCCTGTACAA TGGGCAGAGC GTGGAGGTGG ACGGGCACTC CATGCGGAAG 
CTGATTGCAG ACCTGCAGCC CAACACCGAA TACTCATTTG TGCTGATGAA CCGCGGCAGC 
AGCGCGGGGG GCCTGCAACA CCTCGTGTCC ATCCGCACGG CCCCCGACCT CCTGCCGCAC 
AAGCCACTGC CAGCCTCGGC CTACATCGAG GACGGCCGCT TCACCCTCAC CATGCCCCGC 
GTGCAGGAAC CCGCACTGGT CAGGTGGTTC TACATCATGG TGGTGCCCAT TGACCGCATG 
GGAGGGAGCA TGCTGGCGCC ACAGTGGAGC ACGCCCGAGG AGCTGGAGCT GGACGAGCTG 
CTGGAGGCCA TCGAGCAGGG CGGCGGGGAG CGGCTGCGGC GCCGGCGGCA GACGGAGCGG 
CTGAAGCCAT ACGTGGCCGC TCAGGTGGAC GTTCTCCCGG AGACCTTCAC CTTGGGGGAC 
AAGAAGAACT ATCAAGGCTT CTACAACCGG CCGCTGTCTC CAGACCTGAG CTACCAGTGC 
TTTGTGCTCG CCTCCCTGAA GGAACCCGTG GACCAGAAGC GCTATGCCTG CAGCCCCTAC 
TCCGATGAGA TCGTGGTCCA GGTGACACCA GCCCAGCAGC AGGAGGAACC CGAGCTGCTG 
TGGGTGACGG GCCCCGTCCT GGCAGTCATT CTCATCGTCC TCATTGTCAT CGCCATTCTC 
CTGTTCAAGA GGAAAAGGAC CCACTCCCCA TCGTCCAAGG ATGAGCAGTC AATTGGGCTG 
AAGGACTCCT TGCTGGCCCA CTCCTCCGAC CCTGTGGAGA TGCGGCGGCT CAACTACCAG 
ACCCCAGGTA TGCGAGACCA CCCGCCCATC CCCATCACCG ACCTAGCTGA CAACATCGAG 
CGCCTCAAAG CCAATGACGG CCTCAAGTTC TCCCAGGAGT ACGAGTCAAT TGACCCAGGA 
CAGCAGTTCA CGTGGGAGAA TTCAAACCTG GAGGTGAACA AGCCGAAGAA CCGCTATGCC 
AACGTCATTG CTTACGACCA CTCTCGAGTC ATCCTTACCT CCATCGATGG TGTCCCCGGG 
AGCGACTACA TCAATGCCAA TTACATCGAC GGCTACCGCA AGCAGAACGC CTACATTGCC 
ACGCAGGGCC CGCTGCCTGA GACCATGGGC GATTTCTGGC GGATGGTGTG GGAACAGCGC 
ACAGCCACTG TGGTCATGAT GACGCGGCTG GAGGAGAAGT CCCGGGTGAA ATGTGACCAG 
TACTGGCCAG CTCGTGGCAC CGAGACCTAT GGCCTCATCC AGGTGACCCT GCTGGACACG 
GTGGAGCTGG CCACCTACAC TGTGCGCACC TTCGCGCTCT ACAAGAGTGG CTCCAGTGAG 
AAGCGGGAGC TGCGTCAGTT CCAGTTCATG GCCTGGCCAG ACCACGGAGT CCCCGAGTAC 
CCGACCCCTA TCCTGGCCTT CCTGCGGCGG GTCAAGGCTT GCAACCCCCT GGACGCGGGG 
CCCATGGTGG TGCACTGCAG CGCGGGCGTG GGCCGCACGG GCTGCTTCAT CGTCATCGAC 
GCCATGCTGG AGCGGATGAA GCACGAGAAG ACAGTGGACA TCTACGGCCA CGTGACGTGC 
ATGCGGGCGC AGCGCAACTA CATGGTGCAG ACTGAGGACC AGTACGTGTT CATCCACGAG 
GCGCTGCTGG AGGCCGCCAT GTGTGGACAC ACGGAGGTGC CCGCCCGCAA CCTCTACGCC 
CACATCCAGA AGCTGGGCCA AGTGCCTCCC GGGGAGAGTG TGACTGCCAT GGAGCTGGAG 
TTCAAGTTGC TGGCCAACTC CAAGGCCCAC ACATCCCGCT TCATCAGCGC CAACCTGCCC 
TGCAACAAGT TCAAGAACCG CCTGGTGAAC ATCATGCCCT ACGAACTGAC CCGTGTGTGT 
CTGCAGCCCA TCCGCGGCGT GGAGGGCTCC GACTACATCA ATGCCAGCTT CCTGGATGGC 
TATCGACAGC AGAAGGCCTA CATAGCCACA CAGGGGCCTC TGGCGGAGAG CACCGAGGAT 
TTCTGGCGCA TGTTATGGGA GCACAACTCC ACCATCATCG TCATGCTGAC CAGGCTTCGG 
GAGATGGGCC GGGAGAAATG CCACCAGTAC TGGCCTGCAG AGCGCTCTGC TCGCTACCAG 
TACTTTGTCG TCGACCCGAT GGCTGAGTAC AACATGCCCC AGTATATTCT CCGTGAGTTC 
AAGGTCACGG ATGCCCGGGA TGGGCAGTCG AGGACAATCC GGCAGTTCCA GTTCACAGAC 
TGGCCGGAGC AGGGCGTGCC TAAAACGGGC GAGGGCTTCA TCGACTTCAT CGGGCAGGTG 
CACAAGACCA AGGAGCAGTT CGGGCAGGAC GGGCCCATCA CGGTGCACTG CAGTGCTGGT 
GTGGGCCGCA CTGGGGTGTT CATCACGCTG AGCATTGTCC TGGAGCGGAT GCGCTACGAA 
GGCGTGGTTG ACATGTTCCA GACCGTGAAG ACCCTGCGCA CACAGCGCCC AGCCATGGTG 
CAGACGGAGG ACCAGTATCA GCTGTGCTAC CGCGCGGCCC TGGAGTACCT CGGAAGCTTT 
GACCACTATG CAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_024987579.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843347.1 Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X3, mRNA.

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CloneID	OBa232662
Clone ID Related Accession (Same CDS sequence)	XM_024987579.1
Accession Version	XM_024987579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	receptor-type tyrosine-protein phosphatase F isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCTG AGCCAGCCCC AGGGAGGACG ATGGTGCCCC TTGTCCCTGC ACTTGTGATG 
CTTGGTTTGG TGGCAGGTGC CCATGGCGAC AGCAAACCTG TCTTTGTCAA GGTCCCCGAG 
GACCAGACTG GATTGTCAGG AGGGGTGGCC TCCTTCGTGT GCCAAGCCAC GGGGGAACCC 
AAGCCGCGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTCGAGGTC 
ATCGAGTTTG ATGACGGGGC AGGGTCTGTG CTGCGGATCC AGCCACTGCG GGTGCAGCGA 
GACGAAGCCA TCTATGAGTG CACGGCCACC AACAGTCTGG GGGAGATCAA CACCAGTGCC 
AAGCTCTCAG TGCTCGAAGA GGAACAGCTG CCCCTTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCCCGC ACGGCCACTA TGCTGTGTGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC CGCCGCGAGC 
AACGGCCGCA TCAAGCAACT GCGTTCAGGT GCCTTGCAGA TTGAGAGCAG CGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCTGCAG GCACTCGTTA CTCGGCCCCT 
GCCAACCTAT ATGTGCGAGT GCGCCGCGTG GCTCCGCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGCGG CAGCGTGAAC CTGACGTGCG TCGCCGTTGG CGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAA GAGCTCACCA AGGAGGACGA GATGCCGGTC 
GGCCGCAATG TGCTGGAGCT CAGCAACGTG GTGCGCTCCG CCAACTACAC TTGCGTGGCC 
ATCTCCTCCC TGGGCATGAT CGAGGCCACG GCCCAGGTCA CCGTGAAAGC CCTGCCGAAG 
CCTCCAATTG ACCTTGTGGT GACAGAGACA ACTGCCACCA GCGTCACTCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT ATCCTACTAT GGCATCCAGT ACCGCCCAGC GGGCGCGGAG 
GGCCCCTTCC AGGAGGTGGA CGGCGTGGCC ACCACCCGCT ACAGCATCGG CGGCCTGAGC 
CCTTTCTCAG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGGCG CGGGCCGCCC 
AGCGAGGCGG TGCGGGCGCG CACGGGGGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTAAGCGC CAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCT 
AACGGCCTGG TGCGGGGCTA CCGCGTCTAC TACACCCCCG ACTCCCGCCG CCCTCTGAGC 
GCCTGGCACA AGCACAACAC AGACGCCGGG CTCCTCACCA CTGTGGGCAG CCTGCTGCCT 
GGCATCACCT ACAGCCTGCG TGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCCCCCAGC 
CCCACCATCC AGGTCAAGAC GCAGCAGGGA GTGCCTGCCC AGCCCGCGGA CTTCCAGGCC 
GAGGTGGACT CAGACACCAG GATCCAGCTC TCGTGGCTGC TGCCCCCGCA GGAGCGGATC 
GTCAAGTATG AGCTGGTGTA CTGGGCGGCA GAGGACGAGG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CGTCCTCCTA TACCGTAGAG GACCTGAAGC CCGACACACT GTACCGCTTC 
CAGCTGGCCG CCCGCTCTGA GCTGGGGGTG GGAGTCTTCA CCCCCACCAT TGAGGCTCGC 
ACAGCACAGT CCATGCCCAG TGGGCCACCG CGGAAGGTGG AGGTGGAGCC GCTCAACTCC 
ACTGCCGTGC GCGTCTCCTG GAAGCTGCCC GTCCCCAGCA AGCAGCATGG CCAGATCCGC 
GGCTACCAGG TCACCTACGT GCGGCTGGAG AATGGCGAGC CCCGCGGCGC CCCCATCATC 
CAGGACGTCA TGCTGGCCGA GGCCCAGGAA ACCACCATCA GCGGCCTGAC CCCGGAGACC 
ACCTACTCCA TCACCGTGGC TGCCTACACC ACCAAGGGGG ACGGTGCCCG CAGCAAGCCC 
AAAATTGTCA CGACCACGGG GGCAGTCCCA GGACGGCCCA CCATGATGGT CAGCACCACG 
GCCATGAACA CGGCGCTGCT CCAGTGGCAC CCGCCCAAGG AGCTGCCCGG GGAGCTCCTG 
GGCTACCGGC TGCAGTACCG CCGAGCCGAC GAGGCGCGGC CCAGCACCAT CGATTTTGGC 
AAGGACGACC AGCACTTTAC GGTCACTGGC CTGCACAAAG GGGCCACCTA CATCTTCCGG 
CTCACTGCCA AGAACCGGGC CGGCCTGGGC GAGGAGTTCG AGAAGGAAAT CACGACCCCC 
GAGGACGTGC CCAGCGGTTT CCCCCAAAAC CTGCGCGTGA TAGGGCTGAC CACATCTACC 
ACGGAACTGA TCTGGGACCC CCCAGTGCTG GCGGAGAGGA ACGGGCGCAT CACCAACTAC 
ACCGTGGTGT ACCGTGACAT CAACAGCCAG CAGGAGCTTC AGAACGTCAC TGCCGACACC 
CACCTGACGC TCTCCGGCCT CAAGCCAGAC ACCACTTACG ACATCAAGGT CCGCGCACGC 
ACCAGCAAGG GCGCCGGCCC GCTCAGCCCC AGCATCCAGT CCCGGACCAT GCCCGTGGAG 
CAAGTGTTTG CCAAGAACTT CCGTGTGGAG GCTGCAATGA AGACATCTGT GCTGCTCAGC 
TGGGAGGTGC CCGACTCCTA CAAGTCAGCA GTGCCCTTCA GGATCCTGTA CAATGGGCAG 
AGCGTGGAGG TGGACGGGCA CTCCATGCGG AAGCTGATTG CAGACCTGCA GCCCAACACC 
GAATACTCAT TTGTGCTGAT GAACCGCGGC AGCAGCGCGG GGGGCCTGCA ACACCTCGTG 
TCCATCCGCA CGGCCCCCGA CCTCCTGCCG CACAAGCCAC TGCCAGCCTC GGCCTACATC 
GAGGACGGCC GCTTCACCCT CACCATGCCC CGCGTGCAGG AACCCGCACT GGTCAGGTGG 
TTCTACATCA TGGTGGTGCC CATTGACCGC ATGGGAGGGA GCATGCTGGC GCCACAGTGG 
AGCACGCCCG AGGAGCTGGA GCTGGACGAG CTGCTGGAGG CCATCGAGCA GGGCGGCGGG 
GAGCGGCTGC GGCGCCGGCG GCAGACGGAG CGGCTGAAGC CATACGTGGC CGCTCAGGTG 
GACGTTCTCC CGGAGACCTT CACCTTGGGG GACAAGAAGA ACTATCAAGG CTTCTACAAC 
CGGCCGCTGT CTCCAGACCT GAGCTACCAG TGCTTTGTGC TCGCCTCCCT GAAGGAACCC 
GTGGACCAGA AGCGCTATGC CTGCAGCCCC TACTCCGATG AGATCGTGGT CCAGGTGACA 
CCAGCCCAGC AGCAGGAGGA ACCCGAGCTG CTGTGGGTGA CGGGCCCCGT CCTGGCAGTC 
ATTCTCATCG TCCTCATTGT CATCGCCATT CTCCTGTTCA AGAGGAAAAG GACCCACTCC 
CCATCGTCCA AGGATGAGCA GTCAATTGGG CTGAAGGACT CCTTGCTGGC CCACTCCTCC 
GACCCTGTGG AGATGCGGCG GCTCAACTAC CAGACCCCAG GTATGCGAGA CCACCCGCCC 
ATCCCCATCA CCGACCTAGC TGACAACATC GAGCGCCTCA AAGCCAATGA CGGCCTCAAG 
TTCTCCCAGG AGTACGAGTC AATTGACCCA GGACAGCAGT TCACGTGGGA GAATTCAAAC 
CTGGAGGTGA ACAAGCCGAA GAACCGCTAT GCCAACGTCA TTGCTTACGA CCACTCTCGA 
GTCATCCTTA CCTCCATCGA TGGTGTCCCC GGGAGCGACT ACATCAATGC CAATTACATC 
GACGGCTACC GCAAGCAGAA CGCCTACATT GCCACGCAGG GCCCGCTGCC TGAGACCATG 
GGCGATTTCT GGCGGATGGT GTGGGAACAG CGCACAGCCA CTGTGGTCAT GATGACGCGG 
CTGGAGGAGA AGTCCCGGGT GAAATGTGAC CAGTACTGGC CAGCTCGTGG CACCGAGACC 
TATGGCCTCA TCCAGGTGAC CCTGCTGGAC ACGGTGGAGC TGGCCACCTA CACTGTGCGC 
ACCTTCGCGC TCTACAAGAG TGGCTCCAGT GAGAAGCGGG AGCTGCGTCA GTTCCAGTTC 
ATGGCCTGGC CAGACCACGG AGTCCCCGAG TACCCGACCC CTATCCTGGC CTTCCTGCGG 
CGGGTCAAGG CTTGCAACCC CCTGGACGCG GGGCCCATGG TGGTGCACTG CAGCGCGGGC 
GTGGGCCGCA CGGGCTGCTT CATCGTCATC GACGCCATGC TGGAGCGGAT GAAGCACGAG 
AAGACAGTGG ACATCTACGG CCACGTGACG TGCATGCGGG CGCAGCGCAA CTACATGGTG 
CAGACTGAGG ACCAGTACGT GTTCATCCAC GAGGCGCTGC TGGAGGCCGC CATGTGTGGA 
CACACGGAGG TGCCCGCCCG CAACCTCTAC GCCCACATCC AGAAGCTGGG CCAAGTGCCT 
CCCGGGGAGA GTGTGACTGC CATGGAGCTG GAGTTCAAGT TGCTGGCCAA CTCCAAGGCC 
CACACATCCC GCTTCATCAG CGCCAACCTG CCCTGCAACA AGTTCAAGAA CCGCCTGGTG 
AACATCATGC CCTACGAACT GACCCGTGTG TGTCTGCAGC CCATCCGCGG CGTGGAGGGC 
TCCGACTACA TCAATGCCAG CTTCCTGGAT GGCTATCGAC AGCAGAAGGC CTACATAGCC 
ACACAGGGGC CTCTGGCGGA GAGCACCGAG GATTTCTGGC GCATGTTATG GGAGCACAAC 
TCCACCATCA TCGTCATGCT GACCAGGCTT CGGGAGATGG GCCGGGAGAA ATGCCACCAG 
TACTGGCCTG CAGAGCGCTC TGCTCGCTAC CAGTACTTTG TCGTCGACCC GATGGCTGAG 
TACAACATGC CCCAGTATAT TCTCCGTGAG TTCAAGGTCA CGGATGCCCG GGATGGGCAG 
TCGAGGACAA TCCGGCAGTT CCAGTTCACA GACTGGCCGG AGCAGGGCGT GCCTAAAACG 
GGCGAGGGCT TCATCGACTT CATCGGGCAG GTGCACAAGA CCAAGGAGCA GTTCGGGCAG 
GACGGGCCCA TCACGGTGCA CTGCAGTGCT GGTGTGGGCC GCACTGGGGT GTTCATCACG 
CTGAGCATTG TCCTGGAGCG GATGCGCTAC GAAGGCGTGG TTGACATGTT CCAGACCGTG 
AAGACCCTGC GCACACAGCG CCCAGCCATG GTGCAGACGG AGGACCAGTA TCAGCTGTGC 
TACCGCGCGG CCCTGGAGTA CCTCGGAAGC TTTGACCACT ATGCAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843347.1
CDS	291..5681
Translation	MTPEPAPGRTMVPLVPALVMLGLVAGAHGDSKPVFVKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPLGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPAASNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLVVTETTATSVTLTWDSGNSEPVSYYGIQYRPAGAEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDSRRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEVDSDTRIQLSWLLPPQERIVKYELVYWAAEDEGQQHKVTFDPTSSYTVEDLKPDTLYRFQLAARSELGVGVFTPTIEARTAQSMPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGAPIIQDVMLAEAQETTISGLTPETTYSITVAAYTTKGDGARSKPKIVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYRRADEARPSTIDFGKDDQHFTVTGLHKGATYIFRLTAKNRAGLGEEFEKEITTPEDVPSGFPQNLRVIGLTTSTTELIWDPPVLAERNGRITNYTVVYRDINSQQELQNVTADTHLTLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPVEQVFAKNFRVEAAMKTSVLLSWEVPDSYKSAVPFRILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPLPASAYIEDGRFTLTMPRVQEPALVRWFYIMVVPIDRMGGSMLAPQWSTPEELELDELLEAIEQGGGERLRRRRQTERLKPYVAAQVDVLPETFTLGDKKNYQGFYNRPLSPDLSYQCFVLASLKEPVDQKRYACSPYSDEIVVQVTPAQQQEEPELLWVTGPVLAVILIVLIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSIDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETYGLIQVTLLDTVELATYTVRTFALYKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRAQRNYMVQTEDQYVFIHEALLEAAMCGHTEVPARNLYAHIQKLGQVPPGESVTAMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHNSTIIVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_024987579.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987579.1

ORF Insert Sequence:

ATGACCCCTG AGCCAGCCCC AGGGAGGACG ATGGTGCCCC TTGTCCCTGC ACTTGTGATG 
CTTGGTTTGG TGGCAGGTGC CCATGGCGAC AGCAAACCTG TCTTTGTCAA GGTCCCCGAG 
GACCAGACTG GATTGTCAGG AGGGGTGGCC TCCTTCGTGT GCCAAGCCAC GGGGGAACCC 
AAGCCGCGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTCGAGGTC 
ATCGAGTTTG ATGACGGGGC AGGGTCTGTG CTGCGGATCC AGCCACTGCG GGTGCAGCGA 
GACGAAGCCA TCTATGAGTG CACGGCCACC AACAGTCTGG GGGAGATCAA CACCAGTGCC 
AAGCTCTCAG TGCTCGAAGA GGAACAGCTG CCCCTTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCCCGC ACGGCCACTA TGCTGTGTGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC CGCCGCGAGC 
AACGGCCGCA TCAAGCAACT GCGTTCAGGT GCCTTGCAGA TTGAGAGCAG CGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCTGCAG GCACTCGTTA CTCGGCCCCT 
GCCAACCTAT ATGTGCGAGT GCGCCGCGTG GCTCCGCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGCGG CAGCGTGAAC CTGACGTGCG TCGCCGTTGG CGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAA GAGCTCACCA AGGAGGACGA GATGCCGGTC 
GGCCGCAATG TGCTGGAGCT CAGCAACGTG GTGCGCTCCG CCAACTACAC TTGCGTGGCC 
ATCTCCTCCC TGGGCATGAT CGAGGCCACG GCCCAGGTCA CCGTGAAAGC CCTGCCGAAG 
CCTCCAATTG ACCTTGTGGT GACAGAGACA ACTGCCACCA GCGTCACTCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT ATCCTACTAT GGCATCCAGT ACCGCCCAGC GGGCGCGGAG 
GGCCCCTTCC AGGAGGTGGA CGGCGTGGCC ACCACCCGCT ACAGCATCGG CGGCCTGAGC 
CCTTTCTCAG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGGCG CGGGCCGCCC 
AGCGAGGCGG TGCGGGCGCG CACGGGGGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTAAGCGC CAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCT 
AACGGCCTGG TGCGGGGCTA CCGCGTCTAC TACACCCCCG ACTCCCGCCG CCCTCTGAGC 
GCCTGGCACA AGCACAACAC AGACGCCGGG CTCCTCACCA CTGTGGGCAG CCTGCTGCCT 
GGCATCACCT ACAGCCTGCG TGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCCCCCAGC 
CCCACCATCC AGGTCAAGAC GCAGCAGGGA GTGCCTGCCC AGCCCGCGGA CTTCCAGGCC 
GAGGTGGACT CAGACACCAG GATCCAGCTC TCGTGGCTGC TGCCCCCGCA GGAGCGGATC 
GTCAAGTATG AGCTGGTGTA CTGGGCGGCA GAGGACGAGG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CGTCCTCCTA TACCGTAGAG GACCTGAAGC CCGACACACT GTACCGCTTC 
CAGCTGGCCG CCCGCTCTGA GCTGGGGGTG GGAGTCTTCA CCCCCACCAT TGAGGCTCGC 
ACAGCACAGT CCATGCCCAG TGGGCCACCG CGGAAGGTGG AGGTGGAGCC GCTCAACTCC 
ACTGCCGTGC GCGTCTCCTG GAAGCTGCCC GTCCCCAGCA AGCAGCATGG CCAGATCCGC 
GGCTACCAGG TCACCTACGT GCGGCTGGAG AATGGCGAGC CCCGCGGCGC CCCCATCATC 
CAGGACGTCA TGCTGGCCGA GGCCCAGGAA ACCACCATCA GCGGCCTGAC CCCGGAGACC 
ACCTACTCCA TCACCGTGGC TGCCTACACC ACCAAGGGGG ACGGTGCCCG CAGCAAGCCC 
AAAATTGTCA CGACCACGGG GGCAGTCCCA GGACGGCCCA CCATGATGGT CAGCACCACG 
GCCATGAACA CGGCGCTGCT CCAGTGGCAC CCGCCCAAGG AGCTGCCCGG GGAGCTCCTG 
GGCTACCGGC TGCAGTACCG CCGAGCCGAC GAGGCGCGGC CCAGCACCAT CGATTTTGGC 
AAGGACGACC AGCACTTTAC GGTCACTGGC CTGCACAAAG GGGCCACCTA CATCTTCCGG 
CTCACTGCCA AGAACCGGGC CGGCCTGGGC GAGGAGTTCG AGAAGGAAAT CACGACCCCC 
GAGGACGTGC CCAGCGGTTT CCCCCAAAAC CTGCGCGTGA TAGGGCTGAC CACATCTACC 
ACGGAACTGA TCTGGGACCC CCCAGTGCTG GCGGAGAGGA ACGGGCGCAT CACCAACTAC 
ACCGTGGTGT ACCGTGACAT CAACAGCCAG CAGGAGCTTC AGAACGTCAC TGCCGACACC 
CACCTGACGC TCTCCGGCCT CAAGCCAGAC ACCACTTACG ACATCAAGGT CCGCGCACGC 
ACCAGCAAGG GCGCCGGCCC GCTCAGCCCC AGCATCCAGT CCCGGACCAT GCCCGTGGAG 
CAAGTGTTTG CCAAGAACTT CCGTGTGGAG GCTGCAATGA AGACATCTGT GCTGCTCAGC 
TGGGAGGTGC CCGACTCCTA CAAGTCAGCA GTGCCCTTCA GGATCCTGTA CAATGGGCAG 
AGCGTGGAGG TGGACGGGCA CTCCATGCGG AAGCTGATTG CAGACCTGCA GCCCAACACC 
GAATACTCAT TTGTGCTGAT GAACCGCGGC AGCAGCGCGG GGGGCCTGCA ACACCTCGTG 
TCCATCCGCA CGGCCCCCGA CCTCCTGCCG CACAAGCCAC TGCCAGCCTC GGCCTACATC 
GAGGACGGCC GCTTCACCCT CACCATGCCC CGCGTGCAGG AACCCGCACT GGTCAGGTGG 
TTCTACATCA TGGTGGTGCC CATTGACCGC ATGGGAGGGA GCATGCTGGC GCCACAGTGG 
AGCACGCCCG AGGAGCTGGA GCTGGACGAG CTGCTGGAGG CCATCGAGCA GGGCGGCGGG 
GAGCGGCTGC GGCGCCGGCG GCAGACGGAG CGGCTGAAGC CATACGTGGC CGCTCAGGTG 
GACGTTCTCC CGGAGACCTT CACCTTGGGG GACAAGAAGA ACTATCAAGG CTTCTACAAC 
CGGCCGCTGT CTCCAGACCT GAGCTACCAG TGCTTTGTGC TCGCCTCCCT GAAGGAACCC 
GTGGACCAGA AGCGCTATGC CTGCAGCCCC TACTCCGATG AGATCGTGGT CCAGGTGACA 
CCAGCCCAGC AGCAGGAGGA ACCCGAGCTG CTGTGGGTGA CGGGCCCCGT CCTGGCAGTC 
ATTCTCATCG TCCTCATTGT CATCGCCATT CTCCTGTTCA AGAGGAAAAG GACCCACTCC 
CCATCGTCCA AGGATGAGCA GTCAATTGGG CTGAAGGACT CCTTGCTGGC CCACTCCTCC 
GACCCTGTGG AGATGCGGCG GCTCAACTAC CAGACCCCAG GTATGCGAGA CCACCCGCCC 
ATCCCCATCA CCGACCTAGC TGACAACATC GAGCGCCTCA AAGCCAATGA CGGCCTCAAG 
TTCTCCCAGG AGTACGAGTC AATTGACCCA GGACAGCAGT TCACGTGGGA GAATTCAAAC 
CTGGAGGTGA ACAAGCCGAA GAACCGCTAT GCCAACGTCA TTGCTTACGA CCACTCTCGA 
GTCATCCTTA CCTCCATCGA TGGTGTCCCC GGGAGCGACT ACATCAATGC CAATTACATC 
GACGGCTACC GCAAGCAGAA CGCCTACATT GCCACGCAGG GCCCGCTGCC TGAGACCATG 
GGCGATTTCT GGCGGATGGT GTGGGAACAG CGCACAGCCA CTGTGGTCAT GATGACGCGG 
CTGGAGGAGA AGTCCCGGGT GAAATGTGAC CAGTACTGGC CAGCTCGTGG CACCGAGACC 
TATGGCCTCA TCCAGGTGAC CCTGCTGGAC ACGGTGGAGC TGGCCACCTA CACTGTGCGC 
ACCTTCGCGC TCTACAAGAG TGGCTCCAGT GAGAAGCGGG AGCTGCGTCA GTTCCAGTTC 
ATGGCCTGGC CAGACCACGG AGTCCCCGAG TACCCGACCC CTATCCTGGC CTTCCTGCGG 
CGGGTCAAGG CTTGCAACCC CCTGGACGCG GGGCCCATGG TGGTGCACTG CAGCGCGGGC 
GTGGGCCGCA CGGGCTGCTT CATCGTCATC GACGCCATGC TGGAGCGGAT GAAGCACGAG 
AAGACAGTGG ACATCTACGG CCACGTGACG TGCATGCGGG CGCAGCGCAA CTACATGGTG 
CAGACTGAGG ACCAGTACGT GTTCATCCAC GAGGCGCTGC TGGAGGCCGC CATGTGTGGA 
CACACGGAGG TGCCCGCCCG CAACCTCTAC GCCCACATCC AGAAGCTGGG CCAAGTGCCT 
CCCGGGGAGA GTGTGACTGC CATGGAGCTG GAGTTCAAGT TGCTGGCCAA CTCCAAGGCC 
CACACATCCC GCTTCATCAG CGCCAACCTG CCCTGCAACA AGTTCAAGAA CCGCCTGGTG 
AACATCATGC CCTACGAACT GACCCGTGTG TGTCTGCAGC CCATCCGCGG CGTGGAGGGC 
TCCGACTACA TCAATGCCAG CTTCCTGGAT GGCTATCGAC AGCAGAAGGC CTACATAGCC 
ACACAGGGGC CTCTGGCGGA GAGCACCGAG GATTTCTGGC GCATGTTATG GGAGCACAAC 
TCCACCATCA TCGTCATGCT GACCAGGCTT CGGGAGATGG GCCGGGAGAA ATGCCACCAG 
TACTGGCCTG CAGAGCGCTC TGCTCGCTAC CAGTACTTTG TCGTCGACCC GATGGCTGAG 
TACAACATGC CCCAGTATAT TCTCCGTGAG TTCAAGGTCA CGGATGCCCG GGATGGGCAG 
TCGAGGACAA TCCGGCAGTT CCAGTTCACA GACTGGCCGG AGCAGGGCGT GCCTAAAACG 
GGCGAGGGCT TCATCGACTT CATCGGGCAG GTGCACAAGA CCAAGGAGCA GTTCGGGCAG 
GACGGGCCCA TCACGGTGCA CTGCAGTGCT GGTGTGGGCC GCACTGGGGT GTTCATCACG 
CTGAGCATTG TCCTGGAGCG GATGCGCTAC GAAGGCGTGG TTGACATGTT CCAGACCGTG 
AAGACCCTGC GCACACAGCG CCCAGCCATG GTGCAGACGG AGGACCAGTA TCAGCTGTGC 
TACCGCGCGG CCCTGGAGTA CCTCGGAAGC TTTGACCACT ATGCAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_024987581.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843349.1 Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa232663
Clone ID Related Accession (Same CDS sequence)	XM_024987581.1
Accession Version	XM_024987581.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	receptor-type tyrosine-protein phosphatase F isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATTA GCAAACAGGC TCCAGTACTG TGTACTGGCC AGAGCGTGGC AGATGCTTGG 
TGGAGTTGGG GGATGGGGTG GAGCCCCTGG CCGAGCCCAC AGGCCCGGCT AGACCTCGAG 
TGGGGAGGGC TTGCTATCTG GAGCATGACC TTCAGTTCTG TCCAGGAGGC CTCTGGGCTT 
CTCAGGGAGA GCTTCCCAGA AGCAGCACCT GTACCAGGTG CCTTGCAGAT TGAGAGCAGC 
GAGGAGTCGG ACCAAGGCAA GTACGAGTGT GTGGCGACCA ACTCTGCAGG CACTCGTTAC 
TCGGCCCCTG CCAACCTATA TGTGCGAGTG CGCCGCGTGG CTCCGCGTTT CTCCATCCCT 
CCCAGCAGCC AGGAGGTGAT GCCGGGCGGC AGCGTGAACC TGACGTGCGT CGCCGTTGGC 
GCACCCATGC CCTACGTGAA GTGGATGATG GGGGCCGAAG AGCTCACCAA GGAGGACGAG 
ATGCCGGTCG GCCGCAATGT GCTGGAGCTC AGCAACGTGG TGCGCTCCGC CAACTACACT 
TGCGTGGCCA TCTCCTCCCT GGGCATGATC GAGGCCACGG CCCAGGTCAC CGTGAAAGCC 
CTGCCGAAGC CTCCAATTGA CCTTGTGGTG ACAGAGACAA CTGCCACCAG CGTCACTCTG 
ACCTGGGACT CTGGGAACTC AGAGCCTGTA TCCTACTATG GCATCCAGTA CCGCCCAGCG 
GGCGCGGAGG GCCCCTTCCA GGAGGTGGAC GGCGTGGCCA CCACCCGCTA CAGCATCGGC 
GGCCTGAGCC CTTTCTCAGA GTACGCCTTC CGCGTGCTGG CGGTGAACAG CATCGGGCGC 
GGGCCGCCCA GCGAGGCGGT GCGGGCGCGC ACGGGGGAGC AGGCGCCCTC CAGCCCCCCG 
CGCCGCGTGC AGGCACGCAT GCTAAGCGCC AGCACCATGC TGGTGCAGTG GGAGCCGCCC 
GAGGAGCCTA ACGGCCTGGT GCGGGGCTAC CGCGTCTACT ACACCCCCGA CTCCCGCCGC 
CCTCTGAGCG CCTGGCACAA GCACAACACA GACGCCGGGC TCCTCACCAC TGTGGGCAGC 
CTGCTGCCTG GCATCACCTA CAGCCTGCGT GTGCTGGCCT TCACCGCCGT GGGCGACGGC 
CCCCCCAGCC CCACCATCCA GGTCAAGACG CAGCAGGGAG TGCCTGCCCA GCCCGCGGAC 
TTCCAGGCCG AGGTGGACTC AGACACCAGG ATCCAGCTCT CGTGGCTGCT GCCCCCGCAG 
GAGCGGATCG TCAAGTATGA GCTGGTGTAC TGGGCGGCAG AGGACGAGGG CCAGCAGCAC 
AAGGTGACCT TCGACCCCAC GTCCTCCTAT ACCGTAGAGG ACCTGAAGCC CGACACACTG 
TACCGCTTCC AGCTGGCCGC CCGCTCTGAG CTGGGGGTGG GAGTCTTCAC CCCCACCATT 
GAGGCTCGCA CAGCACAGTC CACCCCCTCC GCACCCCCCC AGAAGGTGAC GTGTGTGAGC 
GTGGGCTCCA CCACCGTCCG GGTAAGTTGG GTCCCGCCGC CGGCCGACAG CCGCAACGGC 
GTCATCACCC AGTACTCGGT GGCCTACGAG GCGGTGGACG GCGAGGACCG TGGGCGGCAC 
GTGGTGGACG GCATCGGCCG CGAGCACTCC AGCTGGGACC TGGTGGGCCT GGAGAAGTGG 
ACGGAGTACC GGGTGTGGGT GCGGGCGCAC ACGGACGTGG GCCCCGGCCC CGAGAGCAGC 
CCGGTGCTGG TCCGCACCGA CGAGGACGTG CCCAGTGGGC CACCGCGGAA GGTGGAGGTG 
GAGCCGCTCA ACTCCACTGC CGTGCGCGTC TCCTGGAAGC TGCCCGTCCC CAGCAAGCAG 
CATGGCCAGA TCCGCGGCTA CCAGGTCACC TACGTGCGGC TGGAGAATGG CGAGCCCCGC 
GGCGCCCCCA TCATCCAGGA CGTCATGCTG GCCGAGGCCC AGTGGCAGCC AGAGGAGTCC 
GAGGACTATG AAACCACCAT CAGCGGCCTG ACCCCGGAGA CCACCTACTC CATCACCGTG 
GCTGCCTACA CCACCAAGGG GGACGGTGCC CGCAGCAAGC CCAAAATTGT CACGACCACG 
GGGGCAGTCC CAGGACGGCC CACCATGATG GTCAGCACCA CGGCCATGAA CACGGCGCTG 
CTCCAGTGGC ACCCGCCCAA GGAGCTGCCC GGGGAGCTCC TGGGCTACCG GCTGCAGTAC 
CGCCGAGCCG ACGAGGCGCG GCCCAGCACC ATCGATTTTG GCAAGGACGA CCAGCACTTT 
ACGGTCACTG GCCTGCACAA AGGGGCCACC TACATCTTCC GGCTCACTGC CAAGAACCGG 
GCCGGCCTGG GCGAGGAGTT CGAGAAGGAA ATCACGACCC CCGAGGACGT GCCCAGCGGT 
TTCCCCCAAA ACCTGCGCGT GATAGGGCTG ACCACATCTA CCACGGAACT GATCTGGGAC 
CCCCCAGTGC TGGCGGAGAG GAACGGGCGC ATCACCAACT ACACCGTGGT GTACCGTGAC 
ATCAACAGCC AGCAGGAGCT TCAGAACGTC ACTGCCGACA CCCACCTGAC GCTCTCCGGC 
CTCAAGCCAG ACACCACTTA CGACATCAAG GTCCGCGCAC GCACCAGCAA GGGCGCCGGC 
CCGCTCAGCC CCAGCATCCA GTCCCGGACC ATGCCCGTGG AGCAAGTGTT TGCCAAGAAC 
TTCCGTGTGG AGGCTGCAAT GAAGACATCT GTGCTGCTCA GCTGGGAGGT GCCCGACTCC 
TACAAGTCAG CAGTGCCCTT CAGGATCCTG TACAATGGGC AGAGCGTGGA GGTGGACGGG 
CACTCCATGC GGAAGCTGAT TGCAGACCTG CAGCCCAACA CCGAATACTC ATTTGTGCTG 
ATGAACCGCG GCAGCAGCGC GGGGGGCCTG CAACACCTCG TGTCCATCCG CACGGCCCCC 
GACCTCCTGC CGCACAAGCC ACTGCCAGCC TCGGCCTACA TCGAGGACGG CCGCTTCACC 
CTCACCATGC CCCGCGTGCA GGAACCCGCA CTGGTCAGGT GGTTCTACAT CATGGTGGTG 
CCCATTGACC GCATGGGAGG GAGCATGCTG GCGCCACAGT GGAGCACGCC CGAGGAGCTG 
GAGCTGGACG AGCTGCTGGA GGCCATCGAG CAGGGCGGCG GGGAGCGGCT GCGGCGCCGG 
CGGCAGACGG AGCGGCTGAA GCCATACGTG GCCGCTCAGG TGGACGTTCT CCCGGAGACC 
TTCACCTTGG GGGACAAGAA GAACTATCAA GGCTTCTACA ACCGGCCGCT GTCTCCAGAC 
CTGAGCTACC AGTGCTTTGT GCTCGCCTCC CTGAAGGAAC CCGTGGACCA GAAGCGCTAT 
GCCTGCAGCC CCTACTCCGA TGAGATCGTG GTCCAGGTGA CACCAGCCCA GCAGCAGGAG 
GAACCCGAGC TGCTGTGGGT GACGGGCCCC GTCCTGGCAG TCATTCTCAT CGTCCTCATT 
GTCATCGCCA TTCTCCTGTT CAAGAGGAAA AGGACCCACT CCCCATCGTC CAAGGATGAG 
CAGTCAATTG GGCTGAAGGA CTCCTTGCTG GCCCACTCCT CCGACCCTGT GGAGATGCGG 
CGGCTCAACT ACCAGACCCC AGGTATGCGA GACCACCCGC CCATCCCCAT CACCGACCTA 
GCTGACAACA TCGAGCGCCT CAAAGCCAAT GACGGCCTCA AGTTCTCCCA GGAGTACGAG 
TCAATTGACC CAGGACAGCA GTTCACGTGG GAGAATTCAA ACCTGGAGGT GAACAAGCCG 
AAGAACCGCT ATGCCAACGT CATTGCTTAC GACCACTCTC GAGTCATCCT TACCTCCATC 
GATGGTGTCC CCGGGAGCGA CTACATCAAT GCCAATTACA TCGACGGCTA CCGCAAGCAG 
AACGCCTACA TTGCCACGCA GGGCCCGCTG CCTGAGACCA TGGGCGATTT CTGGCGGATG 
GTGTGGGAAC AGCGCACAGC CACTGTGGTC ATGATGACGC GGCTGGAGGA GAAGTCCCGG 
GTGAAATGTG ACCAGTACTG GCCAGCTCGT GGCACCGAGA CCTATGGCCT CATCCAGGTG 
ACCCTGCTGG ACACGGTGGA GCTGGCCACC TACACTGTGC GCACCTTCGC GCTCTACAAG 
AGTGGCTCCA GTGAGAAGCG GGAGCTGCGT CAGTTCCAGT TCATGGCCTG GCCAGACCAC 
GGAGTCCCCG AGTACCCGAC CCCTATCCTG GCCTTCCTGC GGCGGGTCAA GGCTTGCAAC 
CCCCTGGACG CGGGGCCCAT GGTGGTGCAC TGCAGCGCGG GCGTGGGCCG CACGGGCTGC 
TTCATCGTCA TCGACGCCAT GCTGGAGCGG ATGAAGCACG AGAAGACAGT GGACATCTAC 
GGCCACGTGA CGTGCATGCG GGCGCAGCGC AACTACATGG TGCAGACTGA GGACCAGTAC 
GTGTTCATCC ACGAGGCGCT GCTGGAGGCC GCCATGTGTG GACACACGGA GGTGCCCGCC 
CGCAACCTCT ACGCCCACAT CCAGAAGCTG GGCCAAGTGC CTCCCGGGGA GAGTGTGACT 
GCCATGGAGC TGGAGTTCAA GTTGCTGGCC AACTCCAAGG CCCACACATC CCGCTTCATC 
AGCGCCAACC TGCCCTGCAA CAAGTTCAAG AACCGCCTGG TGAACATCAT GCCCTACGAA 
CTGACCCGTG TGTGTCTGCA GCCCATCCGC GGCGTGGAGG GCTCCGACTA CATCAATGCC 
AGCTTCCTGG ATGGCTATCG ACAGCAGAAG GCCTACATAG CCACACAGGG GCCTCTGGCG 
GAGAGCACCG AGGATTTCTG GCGCATGTTA TGGGAGCACA ACTCCACCAT CATCGTCATG 
CTGACCAGGC TTCGGGAGAT GGGCCGGGAG AAATGCCACC AGTACTGGCC TGCAGAGCGC 
TCTGCTCGCT ACCAGTACTT TGTCGTCGAC CCGATGGCTG AGTACAACAT GCCCCAGTAT 
ATTCTCCGTG AGTTCAAGGT CACGGATGCC CGGGATGGGC AGTCGAGGAC AATCCGGCAG 
TTCCAGTTCA CAGACTGGCC GGAGCAGGGC GTGCCTAAAA CGGGCGAGGG CTTCATCGAC 
TTCATCGGGC AGGTGCACAA GACCAAGGAG CAGTTCGGGC AGGACGGGCC CATCACGGTG 
CACTGCAGTG CTGGTGTGGG CCGCACTGGG GTGTTCATCA CGCTGAGCAT TGTCCTGGAG 
CGGATGCGCT ACGAAGGCGT GGTTGACATG TTCCAGACCG TGAAGACCCT GCGCACACAG 
CGCCCAGCCA TGGTGCAGAC GGAGGACCAG TATCAGCTGT GCTACCGCGC GGCCCTGGAG 
TACCTCGGAA GCTTTGACCA CTATGCAACG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843349.1
CDS	14..5386
Translation	MLISKQAPVLCTGQSVADAWWSWGMGWSPWPSPQARLDLEWGGLAIWSMTFSSVQEASGLLRESFPEAAPVPGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLVVTETTATSVTLTWDSGNSEPVSYYGIQYRPAGAEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDSRRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEVDSDTRIQLSWLLPPQERIVKYELVYWAAEDEGQQHKVTFDPTSSYTVEDLKPDTLYRFQLAARSELGVGVFTPTIEARTAQSTPSAPPQKVTCVSVGSTTVRVSWVPPPADSRNGVITQYSVAYEAVDGEDRGRHVVDGIGREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGAPIIQDVMLAEAQWQPEESEDYETTISGLTPETTYSITVAAYTTKGDGARSKPKIVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYRRADEARPSTIDFGKDDQHFTVTGLHKGATYIFRLTAKNRAGLGEEFEKEITTPEDVPSGFPQNLRVIGLTTSTTELIWDPPVLAERNGRITNYTVVYRDINSQQELQNVTADTHLTLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPVEQVFAKNFRVEAAMKTSVLLSWEVPDSYKSAVPFRILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPLPASAYIEDGRFTLTMPRVQEPALVRWFYIMVVPIDRMGGSMLAPQWSTPEELELDELLEAIEQGGGERLRRRRQTERLKPYVAAQVDVLPETFTLGDKKNYQGFYNRPLSPDLSYQCFVLASLKEPVDQKRYACSPYSDEIVVQVTPAQQQEEPELLWVTGPVLAVILIVLIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSIDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETYGLIQVTLLDTVELATYTVRTFALYKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRAQRNYMVQTEDQYVFIHEALLEAAMCGHTEVPARNLYAHIQKLGQVPPGESVTAMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHNSTIIVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_024987581.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987581.1

ORF Insert Sequence:

ATGCTCATTA GCAAACAGGC TCCAGTACTG TGTACTGGCC AGAGCGTGGC AGATGCTTGG 
TGGAGTTGGG GGATGGGGTG GAGCCCCTGG CCGAGCCCAC AGGCCCGGCT AGACCTCGAG 
TGGGGAGGGC TTGCTATCTG GAGCATGACC TTCAGTTCTG TCCAGGAGGC CTCTGGGCTT 
CTCAGGGAGA GCTTCCCAGA AGCAGCACCT GTACCAGGTG CCTTGCAGAT TGAGAGCAGC 
GAGGAGTCGG ACCAAGGCAA GTACGAGTGT GTGGCGACCA ACTCTGCAGG CACTCGTTAC 
TCGGCCCCTG CCAACCTATA TGTGCGAGTG CGCCGCGTGG CTCCGCGTTT CTCCATCCCT 
CCCAGCAGCC AGGAGGTGAT GCCGGGCGGC AGCGTGAACC TGACGTGCGT CGCCGTTGGC 
GCACCCATGC CCTACGTGAA GTGGATGATG GGGGCCGAAG AGCTCACCAA GGAGGACGAG 
ATGCCGGTCG GCCGCAATGT GCTGGAGCTC AGCAACGTGG TGCGCTCCGC CAACTACACT 
TGCGTGGCCA TCTCCTCCCT GGGCATGATC GAGGCCACGG CCCAGGTCAC CGTGAAAGCC 
CTGCCGAAGC CTCCAATTGA CCTTGTGGTG ACAGAGACAA CTGCCACCAG CGTCACTCTG 
ACCTGGGACT CTGGGAACTC AGAGCCTGTA TCCTACTATG GCATCCAGTA CCGCCCAGCG 
GGCGCGGAGG GCCCCTTCCA GGAGGTGGAC GGCGTGGCCA CCACCCGCTA CAGCATCGGC 
GGCCTGAGCC CTTTCTCAGA GTACGCCTTC CGCGTGCTGG CGGTGAACAG CATCGGGCGC 
GGGCCGCCCA GCGAGGCGGT GCGGGCGCGC ACGGGGGAGC AGGCGCCCTC CAGCCCCCCG 
CGCCGCGTGC AGGCACGCAT GCTAAGCGCC AGCACCATGC TGGTGCAGTG GGAGCCGCCC 
GAGGAGCCTA ACGGCCTGGT GCGGGGCTAC CGCGTCTACT ACACCCCCGA CTCCCGCCGC 
CCTCTGAGCG CCTGGCACAA GCACAACACA GACGCCGGGC TCCTCACCAC TGTGGGCAGC 
CTGCTGCCTG GCATCACCTA CAGCCTGCGT GTGCTGGCCT TCACCGCCGT GGGCGACGGC 
CCCCCCAGCC CCACCATCCA GGTCAAGACG CAGCAGGGAG TGCCTGCCCA GCCCGCGGAC 
TTCCAGGCCG AGGTGGACTC AGACACCAGG ATCCAGCTCT CGTGGCTGCT GCCCCCGCAG 
GAGCGGATCG TCAAGTATGA GCTGGTGTAC TGGGCGGCAG AGGACGAGGG CCAGCAGCAC 
AAGGTGACCT TCGACCCCAC GTCCTCCTAT ACCGTAGAGG ACCTGAAGCC CGACACACTG 
TACCGCTTCC AGCTGGCCGC CCGCTCTGAG CTGGGGGTGG GAGTCTTCAC CCCCACCATT 
GAGGCTCGCA CAGCACAGTC CACCCCCTCC GCACCCCCCC AGAAGGTGAC GTGTGTGAGC 
GTGGGCTCCA CCACCGTCCG GGTAAGTTGG GTCCCGCCGC CGGCCGACAG CCGCAACGGC 
GTCATCACCC AGTACTCGGT GGCCTACGAG GCGGTGGACG GCGAGGACCG TGGGCGGCAC 
GTGGTGGACG GCATCGGCCG CGAGCACTCC AGCTGGGACC TGGTGGGCCT GGAGAAGTGG 
ACGGAGTACC GGGTGTGGGT GCGGGCGCAC ACGGACGTGG GCCCCGGCCC CGAGAGCAGC 
CCGGTGCTGG TCCGCACCGA CGAGGACGTG CCCAGTGGGC CACCGCGGAA GGTGGAGGTG 
GAGCCGCTCA ACTCCACTGC CGTGCGCGTC TCCTGGAAGC TGCCCGTCCC CAGCAAGCAG 
CATGGCCAGA TCCGCGGCTA CCAGGTCACC TACGTGCGGC TGGAGAATGG CGAGCCCCGC 
GGCGCCCCCA TCATCCAGGA CGTCATGCTG GCCGAGGCCC AGTGGCAGCC AGAGGAGTCC 
GAGGACTATG AAACCACCAT CAGCGGCCTG ACCCCGGAGA CCACCTACTC CATCACCGTG 
GCTGCCTACA CCACCAAGGG GGACGGTGCC CGCAGCAAGC CCAAAATTGT CACGACCACG 
GGGGCAGTCC CAGGACGGCC CACCATGATG GTCAGCACCA CGGCCATGAA CACGGCGCTG 
CTCCAGTGGC ACCCGCCCAA GGAGCTGCCC GGGGAGCTCC TGGGCTACCG GCTGCAGTAC 
CGCCGAGCCG ACGAGGCGCG GCCCAGCACC ATCGATTTTG GCAAGGACGA CCAGCACTTT 
ACGGTCACTG GCCTGCACAA AGGGGCCACC TACATCTTCC GGCTCACTGC CAAGAACCGG 
GCCGGCCTGG GCGAGGAGTT CGAGAAGGAA ATCACGACCC CCGAGGACGT GCCCAGCGGT 
TTCCCCCAAA ACCTGCGCGT GATAGGGCTG ACCACATCTA CCACGGAACT GATCTGGGAC 
CCCCCAGTGC TGGCGGAGAG GAACGGGCGC ATCACCAACT ACACCGTGGT GTACCGTGAC 
ATCAACAGCC AGCAGGAGCT TCAGAACGTC ACTGCCGACA CCCACCTGAC GCTCTCCGGC 
CTCAAGCCAG ACACCACTTA CGACATCAAG GTCCGCGCAC GCACCAGCAA GGGCGCCGGC 
CCGCTCAGCC CCAGCATCCA GTCCCGGACC ATGCCCGTGG AGCAAGTGTT TGCCAAGAAC 
TTCCGTGTGG AGGCTGCAAT GAAGACATCT GTGCTGCTCA GCTGGGAGGT GCCCGACTCC 
TACAAGTCAG CAGTGCCCTT CAGGATCCTG TACAATGGGC AGAGCGTGGA GGTGGACGGG 
CACTCCATGC GGAAGCTGAT TGCAGACCTG CAGCCCAACA CCGAATACTC ATTTGTGCTG 
ATGAACCGCG GCAGCAGCGC GGGGGGCCTG CAACACCTCG TGTCCATCCG CACGGCCCCC 
GACCTCCTGC CGCACAAGCC ACTGCCAGCC TCGGCCTACA TCGAGGACGG CCGCTTCACC 
CTCACCATGC CCCGCGTGCA GGAACCCGCA CTGGTCAGGT GGTTCTACAT CATGGTGGTG 
CCCATTGACC GCATGGGAGG GAGCATGCTG GCGCCACAGT GGAGCACGCC CGAGGAGCTG 
GAGCTGGACG AGCTGCTGGA GGCCATCGAG CAGGGCGGCG GGGAGCGGCT GCGGCGCCGG 
CGGCAGACGG AGCGGCTGAA GCCATACGTG GCCGCTCAGG TGGACGTTCT CCCGGAGACC 
TTCACCTTGG GGGACAAGAA GAACTATCAA GGCTTCTACA ACCGGCCGCT GTCTCCAGAC 
CTGAGCTACC AGTGCTTTGT GCTCGCCTCC CTGAAGGAAC CCGTGGACCA GAAGCGCTAT 
GCCTGCAGCC CCTACTCCGA TGAGATCGTG GTCCAGGTGA CACCAGCCCA GCAGCAGGAG 
GAACCCGAGC TGCTGTGGGT GACGGGCCCC GTCCTGGCAG TCATTCTCAT CGTCCTCATT 
GTCATCGCCA TTCTCCTGTT CAAGAGGAAA AGGACCCACT CCCCATCGTC CAAGGATGAG 
CAGTCAATTG GGCTGAAGGA CTCCTTGCTG GCCCACTCCT CCGACCCTGT GGAGATGCGG 
CGGCTCAACT ACCAGACCCC AGGTATGCGA GACCACCCGC CCATCCCCAT CACCGACCTA 
GCTGACAACA TCGAGCGCCT CAAAGCCAAT GACGGCCTCA AGTTCTCCCA GGAGTACGAG 
TCAATTGACC CAGGACAGCA GTTCACGTGG GAGAATTCAA ACCTGGAGGT GAACAAGCCG 
AAGAACCGCT ATGCCAACGT CATTGCTTAC GACCACTCTC GAGTCATCCT TACCTCCATC 
GATGGTGTCC CCGGGAGCGA CTACATCAAT GCCAATTACA TCGACGGCTA CCGCAAGCAG 
AACGCCTACA TTGCCACGCA GGGCCCGCTG CCTGAGACCA TGGGCGATTT CTGGCGGATG 
GTGTGGGAAC AGCGCACAGC CACTGTGGTC ATGATGACGC GGCTGGAGGA GAAGTCCCGG 
GTGAAATGTG ACCAGTACTG GCCAGCTCGT GGCACCGAGA CCTATGGCCT CATCCAGGTG 
ACCCTGCTGG ACACGGTGGA GCTGGCCACC TACACTGTGC GCACCTTCGC GCTCTACAAG 
AGTGGCTCCA GTGAGAAGCG GGAGCTGCGT CAGTTCCAGT TCATGGCCTG GCCAGACCAC 
GGAGTCCCCG AGTACCCGAC CCCTATCCTG GCCTTCCTGC GGCGGGTCAA GGCTTGCAAC 
CCCCTGGACG CGGGGCCCAT GGTGGTGCAC TGCAGCGCGG GCGTGGGCCG CACGGGCTGC 
TTCATCGTCA TCGACGCCAT GCTGGAGCGG ATGAAGCACG AGAAGACAGT GGACATCTAC 
GGCCACGTGA CGTGCATGCG GGCGCAGCGC AACTACATGG TGCAGACTGA GGACCAGTAC 
GTGTTCATCC ACGAGGCGCT GCTGGAGGCC GCCATGTGTG GACACACGGA GGTGCCCGCC 
CGCAACCTCT ACGCCCACAT CCAGAAGCTG GGCCAAGTGC CTCCCGGGGA GAGTGTGACT 
GCCATGGAGC TGGAGTTCAA GTTGCTGGCC AACTCCAAGG CCCACACATC CCGCTTCATC 
AGCGCCAACC TGCCCTGCAA CAAGTTCAAG AACCGCCTGG TGAACATCAT GCCCTACGAA 
CTGACCCGTG TGTGTCTGCA GCCCATCCGC GGCGTGGAGG GCTCCGACTA CATCAATGCC 
AGCTTCCTGG ATGGCTATCG ACAGCAGAAG GCCTACATAG CCACACAGGG GCCTCTGGCG 
GAGAGCACCG AGGATTTCTG GCGCATGTTA TGGGAGCACA ACTCCACCAT CATCGTCATG 
CTGACCAGGC TTCGGGAGAT GGGCCGGGAG AAATGCCACC AGTACTGGCC TGCAGAGCGC 
TCTGCTCGCT ACCAGTACTT TGTCGTCGAC CCGATGGCTG AGTACAACAT GCCCCAGTAT 
ATTCTCCGTG AGTTCAAGGT CACGGATGCC CGGGATGGGC AGTCGAGGAC AATCCGGCAG 
TTCCAGTTCA CAGACTGGCC GGAGCAGGGC GTGCCTAAAA CGGGCGAGGG CTTCATCGAC 
TTCATCGGGC AGGTGCACAA GACCAAGGAG CAGTTCGGGC AGGACGGGCC CATCACGGTG 
CACTGCAGTG CTGGTGTGGG CCGCACTGGG GTGTTCATCA CGCTGAGCAT TGTCCTGGAG 
CGGATGCGCT ACGAAGGCGT GGTTGACATG TTCCAGACCG TGAAGACCCT GCGCACACAG 
CGCCCAGCCA TGGTGCAGAC GGAGGACCAG TATCAGCTGT GCTACCGCGC GGCCCTGGAG 
TACCTCGGAA GCTTTGACCA CTATGCAACG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_024987584.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843352.1 Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X5, mRNA.

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CloneID	OBa232664
Clone ID Related Accession (Same CDS sequence)	XM_024987584.1
Accession Version	XM_024987584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	receptor-type tyrosine-protein phosphatase F isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCTG AGCCAGCCCC AGGGAGGACG ATGGTGCCCC TTGTCCCTGC ACTTGTGATG 
CTTGGTTTGG TGGCAGGTGC CCATGGCGAC AGCAAACCTG TCTTTGTCAA GGTCCCCGAG 
GACCAGACTG GATTGTCAGG AGGGGTGGCC TCCTTCGTGT GCCAAGCCAC GGGGGAACCC 
AAGCCGCGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTCGAGGTC 
ATCGAGTTTG ATGACGGGGC AGGGTCTGTG CTGCGGATCC AGCCACTGCG GGTGCAGCGA 
GACGAAGCCA TCTATGAGTG CACGGCCACC AACAGTCTGG GGGAGATCAA CACCAGTGCC 
AAGCTCTCAG TGCTCGAAGA GGAACAGCTG CCCCTTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCCCGC ACGGCCACTA TGCTGTGTGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC CGCCGCGAGC 
AACGGCCGCA TCAAGCAACT GCGTTCAGGT GCCTTGCAGA TTGAGAGCAG CGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCTGCAG GCACTCGTTA CTCGGCCCCT 
GCCAACCTAT ATGTGCGAGT GCGCCGCGTG GCTCCGCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGCGG CAGCGTGAAC CTGACGTGCG TCGCCGTTGG CGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAA GAGCTCACCA AGGAGGACGA GATGCCGGTC 
GGCCGCAATG TGCTGGAGCT CAGCAACGTG GTGCGCTCCG CCAACTACAC TTGCGTGGCC 
ATCTCCTCCC TGGGCATGAT CGAGGCCACG GCCCAGGTCA CCGTGAAAGC CCTGCCGAAG 
CCTCCAATTG ACCTTGTGGT GACAGAGACA ACTGCCACCA GCGTCACTCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT ATCCTACTAT GGCATCCAGT ACCGCCCAGC GGGCGCGGAG 
GGCCCCTTCC AGGAGGTGGA CGGCGTGGCC ACCACCCGCT ACAGCATCGG CGGCCTGAGC 
CCTTTCTCAG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGGCG CGGGCCGCCC 
AGCGAGGCGG TGCGGGCGCG CACGGGGGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTAAGCGC CAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCT 
AACGGCCTGG TGCGGGGCTA CCGCGTCTAC TACACCCCCG ACTCCCGCCG CCCTCTGAGC 
GCCTGGCACA AGCACAACAC AGACGCCGGG CTCCTCACCA CTGTGGGCAG CCTGCTGCCT 
GGCATCACCT ACAGCCTGCG TGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCCCCCAGC 
CCCACCATCC AGGTCAAGAC GCAGCAGGGA GTGCCTGCCC AGCCCGCGGA CTTCCAGGCC 
GAGGTGGACT CAGACACCAG GATCCAGCTC TCGTGGCTGC TGCCCCCGCA GGAGCGGATC 
GTCAAGTATG AGCTGGTGTA CTGGGCGGCA GAGGACGAGG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CGTCCTCCTA TACCGTAGAG GACCTGAAGC CCGACACACT GTACCGCTTC 
CAGCTGGCCG CCCGCTCTGA GCTGGGGGTG GGAGTCTTCA CCCCCACCAT TGAGGCTCGC 
ACAGCACAGT CCACCCCCTC CGCACCCCCC CAGAAGGTGA CGTGTGTGAG CGTGGGCTCC 
ACCACCGTCC GGGTAAGTTG GGTCCCGCCG CCGGCCGACA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCGGTGGAC GGCGAGGACC GTGGGCGGCA CGTGGTGGAC 
GGCATCGGCC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACGGACGTG GGCCCCGGCC CCGAGAGCAG CCCGGTGCTG 
GTCCGCACCG ACGAGGACGT GCCCAGTGGG CCACCGCGGA AGGTGGAGGT GGAGCCGCTC 
AACTCCACTG CCGTGCGCGT CTCCTGGAAG CTGCCCGTCC CCAGCAAGCA GCATGGCCAG 
ATCCGCGGCT ACCAGGTCAC CTACGTGCGG CTGGAGAATG GCGAGCCCCG CGGCGCCCCC 
ATCATCCAGG ACGTCATGCT GGCCGAGGCC CAGTGGCAGC CAGAGGAGTC CGAGGACTAT 
GAAACCACCA TCAGCGGCCT GACCCCGGAG ACCACCTACT CCATCACCGT GGCTGCCTAC 
ACCACCAAGG GGGACGGTGC CCGCAGCAAG CCCAAAATTG TCACGACCAC GGGGGCAGTG 
TTTGCCAAGA ACTTCCGTGT GGAGGCTGCA ATGAAGACAT CTGTGCTGCT CAGCTGGGAG 
GTGCCCGACT CCTACAAGTC AGCAGTGCCC TTCAGGATCC TGTACAATGG GCAGAGCGTG 
GAGGTGGACG GGCACTCCAT GCGGAAGCTG ATTGCAGACC TGCAGCCCAA CACCGAATAC 
TCATTTGTGC TGATGAACCG CGGCAGCAGC GCGGGGGGCC TGCAACACCT CGTGTCCATC 
CGCACGGCCC CCGACCTCCT GCCGCACAAG CCACTGCCAG CCTCGGCCTA CATCGAGGAC 
GGCCGCTTCA CCCTCACCAT GCCCCGCGTG CAGGAACCCG CACTGGTCAG GTGGTTCTAC 
ATCATGGTGG TGCCCATTGA CCGCATGGGA GGGAGCATGC TGGCGCCACA GTGGAGCACG 
CCCGAGGAGC TGGAGCTGGA CGAGCTGCTG GAGGCCATCG AGCAGGGCGG CGGGGAGCGG 
CTGCGGCGCC GGCGGCAGAC GGAGCGGCTG AAGCCATACG TGGCCGCTCA GGTGGACGTT 
CTCCCGGAGA CCTTCACCTT GGGGGACAAG AAGAACTATC AAGGCTTCTA CAACCGGCCG 
CTGTCTCCAG ACCTGAGCTA CCAGTGCTTT GTGCTCGCCT CCCTGAAGGA ACCCGTGGAC 
CAGAAGCGCT ATGCCTGCAG CCCCTACTCC GATGAGATCG TGGTCCAGGT GACACCAGCC 
CAGCAGCAGG AGGAACCCGA GCTGCTGTGG GTGACGGGCC CCGTCCTGGC AGTCATTCTC 
ATCGTCCTCA TTGTCATCGC CATTCTCCTG TTCAAGAGGA AAAGGACCCA CTCCCCATCG 
TCCAAGGATG AGCAGTCAAT TGGGCTGAAG GACTCCTTGC TGGCCCACTC CTCCGACCCT 
GTGGAGATGC GGCGGCTCAA CTACCAGACC CCAGGTATGC GAGACCACCC GCCCATCCCC 
ATCACCGACC TAGCTGACAA CATCGAGCGC CTCAAAGCCA ATGACGGCCT CAAGTTCTCC 
CAGGAGTACG AGTCAATTGA CCCAGGACAG CAGTTCACGT GGGAGAATTC AAACCTGGAG 
GTGAACAAGC CGAAGAACCG CTATGCCAAC GTCATTGCTT ACGACCACTC TCGAGTCATC 
CTTACCTCCA TCGATGGTGT CCCCGGGAGC GACTACATCA ATGCCAATTA CATCGACGGC 
TACCGCAAGC AGAACGCCTA CATTGCCACG CAGGGCCCGC TGCCTGAGAC CATGGGCGAT 
TTCTGGCGGA TGGTGTGGGA ACAGCGCACA GCCACTGTGG TCATGATGAC GCGGCTGGAG 
GAGAAGTCCC GGGTGAAATG TGACCAGTAC TGGCCAGCTC GTGGCACCGA GACCTATGGC 
CTCATCCAGG TGACCCTGCT GGACACGGTG GAGCTGGCCA CCTACACTGT GCGCACCTTC 
GCGCTCTACA AGAGTGGCTC CAGTGAGAAG CGGGAGCTGC GTCAGTTCCA GTTCATGGCC 
TGGCCAGACC ACGGAGTCCC CGAGTACCCG ACCCCTATCC TGGCCTTCCT GCGGCGGGTC 
AAGGCTTGCA ACCCCCTGGA CGCGGGGCCC ATGGTGGTGC ACTGCAGCGC GGGCGTGGGC 
CGCACGGGCT GCTTCATCGT CATCGACGCC ATGCTGGAGC GGATGAAGCA CGAGAAGACA 
GTGGACATCT ACGGCCACGT GACGTGCATG CGGGCGCAGC GCAACTACAT GGTGCAGACT 
GAGGACCAGT ACGTGTTCAT CCACGAGGCG CTGCTGGAGG CCGCCATGTG TGGACACACG 
GAGGTGCCCG CCCGCAACCT CTACGCCCAC ATCCAGAAGC TGGGCCAAGT GCCTCCCGGG 
GAGAGTGTGA CTGCCATGGA GCTGGAGTTC AAGTTGCTGG CCAACTCCAA GGCCCACACA 
TCCCGCTTCA TCAGCGCCAA CCTGCCCTGC AACAAGTTCA AGAACCGCCT GGTGAACATC 
ATGCCCTACG AACTGACCCG TGTGTGTCTG CAGCCCATCC GCGGCGTGGA GGGCTCCGAC 
TACATCAATG CCAGCTTCCT GGATGGCTAT CGACAGCAGA AGGCCTACAT AGCCACACAG 
GGGCCTCTGG CGGAGAGCAC CGAGGATTTC TGGCGCATGT TATGGGAGCA CAACTCCACC 
ATCATCGTCA TGCTGACCAG GCTTCGGGAG ATGGGCCGGG AGAAATGCCA CCAGTACTGG 
CCTGCAGAGC GCTCTGCTCG CTACCAGTAC TTTGTCGTCG ACCCGATGGC TGAGTACAAC 
ATGCCCCAGT ATATTCTCCG TGAGTTCAAG GTCACGGATG CCCGGGATGG GCAGTCGAGG 
ACAATCCGGC AGTTCCAGTT CACAGACTGG CCGGAGCAGG GCGTGCCTAA AACGGGCGAG 
GGCTTCATCG ACTTCATCGG GCAGGTGCAC AAGACCAAGG AGCAGTTCGG GCAGGACGGG 
CCCATCACGG TGCACTGCAG TGCTGGTGTG GGCCGCACTG GGGTGTTCAT CACGCTGAGC 
ATTGTCCTGG AGCGGATGCG CTACGAAGGC GTGGTTGACA TGTTCCAGAC CGTGAAGACC 
CTGCGCACAC AGCGCCCAGC CATGGTGCAG ACGGAGGACC AGTATCAGCT GTGCTACCGC 
GCGGCCCTGG AGTACCTCGG AAGCTTTGAC CACTATGCAA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843352.1
CDS	292..5436
Translation	MTPEPAPGRTMVPLVPALVMLGLVAGAHGDSKPVFVKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPLGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPAASNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLVVTETTATSVTLTWDSGNSEPVSYYGIQYRPAGAEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDSRRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEVDSDTRIQLSWLLPPQERIVKYELVYWAAEDEGQQHKVTFDPTSSYTVEDLKPDTLYRFQLAARSELGVGVFTPTIEARTAQSTPSAPPQKVTCVSVGSTTVRVSWVPPPADSRNGVITQYSVAYEAVDGEDRGRHVVDGIGREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGAPIIQDVMLAEAQWQPEESEDYETTISGLTPETTYSITVAAYTTKGDGARSKPKIVTTTGAVFAKNFRVEAAMKTSVLLSWEVPDSYKSAVPFRILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPLPASAYIEDGRFTLTMPRVQEPALVRWFYIMVVPIDRMGGSMLAPQWSTPEELELDELLEAIEQGGGERLRRRRQTERLKPYVAAQVDVLPETFTLGDKKNYQGFYNRPLSPDLSYQCFVLASLKEPVDQKRYACSPYSDEIVVQVTPAQQQEEPELLWVTGPVLAVILIVLIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSIDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETYGLIQVTLLDTVELATYTVRTFALYKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRAQRNYMVQTEDQYVFIHEALLEAAMCGHTEVPARNLYAHIQKLGQVPPGESVTAMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHNSTIIVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_024987584.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987584.1

ORF Insert Sequence:

ATGACCCCTG AGCCAGCCCC AGGGAGGACG ATGGTGCCCC TTGTCCCTGC ACTTGTGATG 
CTTGGTTTGG TGGCAGGTGC CCATGGCGAC AGCAAACCTG TCTTTGTCAA GGTCCCCGAG 
GACCAGACTG GATTGTCAGG AGGGGTGGCC TCCTTCGTGT GCCAAGCCAC GGGGGAACCC 
AAGCCGCGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTCGAGGTC 
ATCGAGTTTG ATGACGGGGC AGGGTCTGTG CTGCGGATCC AGCCACTGCG GGTGCAGCGA 
GACGAAGCCA TCTATGAGTG CACGGCCACC AACAGTCTGG GGGAGATCAA CACCAGTGCC 
AAGCTCTCAG TGCTCGAAGA GGAACAGCTG CCCCTTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCCCGC ACGGCCACTA TGCTGTGTGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC CGCCGCGAGC 
AACGGCCGCA TCAAGCAACT GCGTTCAGGT GCCTTGCAGA TTGAGAGCAG CGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCTGCAG GCACTCGTTA CTCGGCCCCT 
GCCAACCTAT ATGTGCGAGT GCGCCGCGTG GCTCCGCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGCGG CAGCGTGAAC CTGACGTGCG TCGCCGTTGG CGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAA GAGCTCACCA AGGAGGACGA GATGCCGGTC 
GGCCGCAATG TGCTGGAGCT CAGCAACGTG GTGCGCTCCG CCAACTACAC TTGCGTGGCC 
ATCTCCTCCC TGGGCATGAT CGAGGCCACG GCCCAGGTCA CCGTGAAAGC CCTGCCGAAG 
CCTCCAATTG ACCTTGTGGT GACAGAGACA ACTGCCACCA GCGTCACTCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT ATCCTACTAT GGCATCCAGT ACCGCCCAGC GGGCGCGGAG 
GGCCCCTTCC AGGAGGTGGA CGGCGTGGCC ACCACCCGCT ACAGCATCGG CGGCCTGAGC 
CCTTTCTCAG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGGCG CGGGCCGCCC 
AGCGAGGCGG TGCGGGCGCG CACGGGGGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTAAGCGC CAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCT 
AACGGCCTGG TGCGGGGCTA CCGCGTCTAC TACACCCCCG ACTCCCGCCG CCCTCTGAGC 
GCCTGGCACA AGCACAACAC AGACGCCGGG CTCCTCACCA CTGTGGGCAG CCTGCTGCCT 
GGCATCACCT ACAGCCTGCG TGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCCCCCAGC 
CCCACCATCC AGGTCAAGAC GCAGCAGGGA GTGCCTGCCC AGCCCGCGGA CTTCCAGGCC 
GAGGTGGACT CAGACACCAG GATCCAGCTC TCGTGGCTGC TGCCCCCGCA GGAGCGGATC 
GTCAAGTATG AGCTGGTGTA CTGGGCGGCA GAGGACGAGG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CGTCCTCCTA TACCGTAGAG GACCTGAAGC CCGACACACT GTACCGCTTC 
CAGCTGGCCG CCCGCTCTGA GCTGGGGGTG GGAGTCTTCA CCCCCACCAT TGAGGCTCGC 
ACAGCACAGT CCACCCCCTC CGCACCCCCC CAGAAGGTGA CGTGTGTGAG CGTGGGCTCC 
ACCACCGTCC GGGTAAGTTG GGTCCCGCCG CCGGCCGACA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCGGTGGAC GGCGAGGACC GTGGGCGGCA CGTGGTGGAC 
GGCATCGGCC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACGGACGTG GGCCCCGGCC CCGAGAGCAG CCCGGTGCTG 
GTCCGCACCG ACGAGGACGT GCCCAGTGGG CCACCGCGGA AGGTGGAGGT GGAGCCGCTC 
AACTCCACTG CCGTGCGCGT CTCCTGGAAG CTGCCCGTCC CCAGCAAGCA GCATGGCCAG 
ATCCGCGGCT ACCAGGTCAC CTACGTGCGG CTGGAGAATG GCGAGCCCCG CGGCGCCCCC 
ATCATCCAGG ACGTCATGCT GGCCGAGGCC CAGTGGCAGC CAGAGGAGTC CGAGGACTAT 
GAAACCACCA TCAGCGGCCT GACCCCGGAG ACCACCTACT CCATCACCGT GGCTGCCTAC 
ACCACCAAGG GGGACGGTGC CCGCAGCAAG CCCAAAATTG TCACGACCAC GGGGGCAGTG 
TTTGCCAAGA ACTTCCGTGT GGAGGCTGCA ATGAAGACAT CTGTGCTGCT CAGCTGGGAG 
GTGCCCGACT CCTACAAGTC AGCAGTGCCC TTCAGGATCC TGTACAATGG GCAGAGCGTG 
GAGGTGGACG GGCACTCCAT GCGGAAGCTG ATTGCAGACC TGCAGCCCAA CACCGAATAC 
TCATTTGTGC TGATGAACCG CGGCAGCAGC GCGGGGGGCC TGCAACACCT CGTGTCCATC 
CGCACGGCCC CCGACCTCCT GCCGCACAAG CCACTGCCAG CCTCGGCCTA CATCGAGGAC 
GGCCGCTTCA CCCTCACCAT GCCCCGCGTG CAGGAACCCG CACTGGTCAG GTGGTTCTAC 
ATCATGGTGG TGCCCATTGA CCGCATGGGA GGGAGCATGC TGGCGCCACA GTGGAGCACG 
CCCGAGGAGC TGGAGCTGGA CGAGCTGCTG GAGGCCATCG AGCAGGGCGG CGGGGAGCGG 
CTGCGGCGCC GGCGGCAGAC GGAGCGGCTG AAGCCATACG TGGCCGCTCA GGTGGACGTT 
CTCCCGGAGA CCTTCACCTT GGGGGACAAG AAGAACTATC AAGGCTTCTA CAACCGGCCG 
CTGTCTCCAG ACCTGAGCTA CCAGTGCTTT GTGCTCGCCT CCCTGAAGGA ACCCGTGGAC 
CAGAAGCGCT ATGCCTGCAG CCCCTACTCC GATGAGATCG TGGTCCAGGT GACACCAGCC 
CAGCAGCAGG AGGAACCCGA GCTGCTGTGG GTGACGGGCC CCGTCCTGGC AGTCATTCTC 
ATCGTCCTCA TTGTCATCGC CATTCTCCTG TTCAAGAGGA AAAGGACCCA CTCCCCATCG 
TCCAAGGATG AGCAGTCAAT TGGGCTGAAG GACTCCTTGC TGGCCCACTC CTCCGACCCT 
GTGGAGATGC GGCGGCTCAA CTACCAGACC CCAGGTATGC GAGACCACCC GCCCATCCCC 
ATCACCGACC TAGCTGACAA CATCGAGCGC CTCAAAGCCA ATGACGGCCT CAAGTTCTCC 
CAGGAGTACG AGTCAATTGA CCCAGGACAG CAGTTCACGT GGGAGAATTC AAACCTGGAG 
GTGAACAAGC CGAAGAACCG CTATGCCAAC GTCATTGCTT ACGACCACTC TCGAGTCATC 
CTTACCTCCA TCGATGGTGT CCCCGGGAGC GACTACATCA ATGCCAATTA CATCGACGGC 
TACCGCAAGC AGAACGCCTA CATTGCCACG CAGGGCCCGC TGCCTGAGAC CATGGGCGAT 
TTCTGGCGGA TGGTGTGGGA ACAGCGCACA GCCACTGTGG TCATGATGAC GCGGCTGGAG 
GAGAAGTCCC GGGTGAAATG TGACCAGTAC TGGCCAGCTC GTGGCACCGA GACCTATGGC 
CTCATCCAGG TGACCCTGCT GGACACGGTG GAGCTGGCCA CCTACACTGT GCGCACCTTC 
GCGCTCTACA AGAGTGGCTC CAGTGAGAAG CGGGAGCTGC GTCAGTTCCA GTTCATGGCC 
TGGCCAGACC ACGGAGTCCC CGAGTACCCG ACCCCTATCC TGGCCTTCCT GCGGCGGGTC 
AAGGCTTGCA ACCCCCTGGA CGCGGGGCCC ATGGTGGTGC ACTGCAGCGC GGGCGTGGGC 
CGCACGGGCT GCTTCATCGT CATCGACGCC ATGCTGGAGC GGATGAAGCA CGAGAAGACA 
GTGGACATCT ACGGCCACGT GACGTGCATG CGGGCGCAGC GCAACTACAT GGTGCAGACT 
GAGGACCAGT ACGTGTTCAT CCACGAGGCG CTGCTGGAGG CCGCCATGTG TGGACACACG 
GAGGTGCCCG CCCGCAACCT CTACGCCCAC ATCCAGAAGC TGGGCCAAGT GCCTCCCGGG 
GAGAGTGTGA CTGCCATGGA GCTGGAGTTC AAGTTGCTGG CCAACTCCAA GGCCCACACA 
TCCCGCTTCA TCAGCGCCAA CCTGCCCTGC AACAAGTTCA AGAACCGCCT GGTGAACATC 
ATGCCCTACG AACTGACCCG TGTGTGTCTG CAGCCCATCC GCGGCGTGGA GGGCTCCGAC 
TACATCAATG CCAGCTTCCT GGATGGCTAT CGACAGCAGA AGGCCTACAT AGCCACACAG 
GGGCCTCTGG CGGAGAGCAC CGAGGATTTC TGGCGCATGT TATGGGAGCA CAACTCCACC 
ATCATCGTCA TGCTGACCAG GCTTCGGGAG ATGGGCCGGG AGAAATGCCA CCAGTACTGG 
CCTGCAGAGC GCTCTGCTCG CTACCAGTAC TTTGTCGTCG ACCCGATGGC TGAGTACAAC 
ATGCCCCAGT ATATTCTCCG TGAGTTCAAG GTCACGGATG CCCGGGATGG GCAGTCGAGG 
ACAATCCGGC AGTTCCAGTT CACAGACTGG CCGGAGCAGG GCGTGCCTAA AACGGGCGAG 
GGCTTCATCG ACTTCATCGG GCAGGTGCAC AAGACCAAGG AGCAGTTCGG GCAGGACGGG 
CCCATCACGG TGCACTGCAG TGCTGGTGTG GGCCGCACTG GGGTGTTCAT CACGCTGAGC 
ATTGTCCTGG AGCGGATGCG CTACGAAGGC GTGGTTGACA TGTTCCAGAC CGTGAAGACC 
CTGCGCACAC AGCGCCCAGC CATGGTGCAG ACGGAGGACC AGTATCAGCT GTGCTACCGC 
GCGGCCCTGG AGTACCTCGG AAGCTTTGAC CACTATGCAA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_024987591.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843359.1 Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X6, mRNA.

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CloneID	OBa232665
Clone ID Related Accession (Same CDS sequence)	XM_024987591.1
Accession Version	XM_024987591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	receptor-type tyrosine-protein phosphatase F isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCTG AGCCAGCCCC AGGGAGGACG ATGGTGCCCC TTGTCCCTGC ACTTGTGATG 
CTTGGTTTGG TGGCAGGTGC CCATGGCGAC AGCAAACCTG TCTTTGTCAA GGTCCCCGAG 
GACCAGACTG GATTGTCAGG AGGGGTGGCC TCCTTCGTGT GCCAAGCCAC GGGGGAACCC 
AAGCCGCGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTCGAGGTC 
ATCGAGTTTG ATGACGGGGC AGGGTCTGTG CTGCGGATCC AGCCACTGCG GGTGCAGCGA 
GACGAAGCCA TCTATGAGTG CACGGCCACC AACAGTCTGG GGGAGATCAA CACCAGTGCC 
AAGCTCTCAG TGCTCGAAGA GGAACAGCTG CCCCTTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCCCGC ACGGCCACTA TGCTGTGTGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC CGCCGCGAGC 
AACGGCCGCA TCAAGCAACT GCGTTCAGGT GCCTTGCAGA TTGAGAGCAG CGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCTGCAG GCACTCGTTA CTCGGCCCCT 
GCCAACCTAT ATGTGCGAGT GCGCCGCGTG GCTCCGCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGCGG CAGCGTGAAC CTGACGTGCG TCGCCGTTGG CGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAA GAGCTCACCA AGGAGGACGA GATGCCGGTC 
GGCCGCAATG TGCTGGAGCT CAGCAACGTG GTGCGCTCCG CCAACTACAC TTGCGTGGCC 
ATCTCCTCCC TGGGCATGAT CGAGGCCACG GCCCAGGTCA CCGTGAAAGC CCTGCCGAAG 
CCTCCAATTG ACCTTGTGGT GACAGAGACA ACTGCCACCA GCGTCACTCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT ATCCTACTAT GGCATCCAGT ACCGCCCAGC GGGCGCGGAG 
GGCCCCTTCC AGGAGGTGGA CGGCGTGGCC ACCACCCGCT ACAGCATCGG CGGCCTGAGC 
CCTTTCTCAG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGGCG CGGGCCGCCC 
AGCGAGGCGG TGCGGGCGCG CACGGGGGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTAAGCGC CAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCT 
AACGGCCTGG TGCGGGGCTA CCGCGTCTAC TACACCCCCG ACTCCCGCCG CCCTCTGAGC 
GCCTGGCACA AGCACAACAC AGACGCCGGG CTCCTCACCA CTGTGGGCAG CCTGCTGCCT 
GGCATCACCT ACAGCCTGCG TGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCCCCCAGC 
CCCACCATCC AGGTCAAGAC GCAGCAGGGA GTGCCTGCCC AGCCCGCGGA CTTCCAGGCC 
GAGGTGGACT CAGACACCAG GATCCAGCTC TCGTGGCTGC TGCCCCCGCA GGAGCGGATC 
GTCAAGTATG AGCTGGTGTA CTGGGCGGCA GAGGACGAGG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CGTCCTCCTA TACCGTAGAG GACCTGAAGC CCGACACACT GTACCGCTTC 
CAGCTGGCCG CCCGCTCTGA GCTGGGGGTG GGAGTCTTCA CCCCCACCAT TGAGGCTCGC 
ACAGCACAGT CCATGCCCAG TGGGCCACCG CGGAAGGTGG AGGTGGAGCC GCTCAACTCC 
ACTGCCGTGC GCGTCTCCTG GAAGCTGCCC GTCCCCAGCA AGCAGCATGG CCAGATCCGC 
GGCTACCAGG TCACCTACGT GCGGCTGGAG AATGGCGAGC CCCGCGGCGC CCCCATCATC 
CAGGACGTCA TGCTGGCCGA GGCCCAGTGG CAGCCAGAGG AGTCCGAGGA CTATGAAACC 
ACCATCAGCG GCCTGACCCC GGAGACCACC TACTCCATCA CCGTGGCTGC CTACACCACC 
AAGGGGGACG GTGCCCGCAG CAAGCCCAAA ATTGTCACGA CCACGGGGGC AGTGTTTGCC 
AAGAACTTCC GTGTGGAGGC TGCAATGAAG ACATCTGTGC TGCTCAGCTG GGAGGTGCCC 
GACTCCTACA AGTCAGCAGT GCCCTTCAGG ATCCTGTACA ATGGGCAGAG CGTGGAGGTG 
GACGGGCACT CCATGCGGAA GCTGATTGCA GACCTGCAGC CCAACACCGA ATACTCATTT 
GTGCTGATGA ACCGCGGCAG CAGCGCGGGG GGCCTGCAAC ACCTCGTGTC CATCCGCACG 
GCCCCCGACC TCCTGCCGCA CAAGCCACTG CCAGCCTCGG CCTACATCGA GGACGGCCGC 
TTCACCCTCA CCATGCCCCG CGTGCAGGAA CCCGCACTGG TCAGGTGGTT CTACATCATG 
GTGGTGCCCA TTGACCGCAT GGGAGGGAGC ATGCTGGCGC CACAGTGGAG CACGCCCGAG 
GAGCTGGAGC TGGACGAGCT GCTGGAGGCC ATCGAGCAGG GCGGCGGGGA GCGGCTGCGG 
CGCCGGCGGC AGACGGAGCG GCTGAAGCCA TACGTGGCCG CTCAGGTGGA CGTTCTCCCG 
GAGACCTTCA CCTTGGGGGA CAAGAAGAAC TATCAAGGCT TCTACAACCG GCCGCTGTCT 
CCAGACCTGA GCTACCAGTG CTTTGTGCTC GCCTCCCTGA AGGAACCCGT GGACCAGAAG 
CGCTATGCCT GCAGCCCCTA CTCCGATGAG ATCGTGGTCC AGGTGACACC AGCCCAGCAG 
CAGGAGGAAC CCGAGCTGCT GTGGGTGACG GGCCCCGTCC TGGCAGTCAT TCTCATCGTC 
CTCATTGTCA TCGCCATTCT CCTGTTCAAG AGGAAAAGGA CCCACTCCCC ATCGTCCAAG 
GATGAGCAGT CAATTGGGCT GAAGGACTCC TTGCTGGCCC ACTCCTCCGA CCCTGTGGAG 
ATGCGGCGGC TCAACTACCA GACCCCAGGT ATGCGAGACC ACCCGCCCAT CCCCATCACC 
GACCTAGCTG ACAACATCGA GCGCCTCAAA GCCAATGACG GCCTCAAGTT CTCCCAGGAG 
TACGAGTCAA TTGACCCAGG ACAGCAGTTC ACGTGGGAGA ATTCAAACCT GGAGGTGAAC 
AAGCCGAAGA ACCGCTATGC CAACGTCATT GCTTACGACC ACTCTCGAGT CATCCTTACC 
TCCATCGATG GTGTCCCCGG GAGCGACTAC ATCAATGCCA ATTACATCGA CGGCTACCGC 
AAGCAGAACG CCTACATTGC CACGCAGGGC CCGCTGCCTG AGACCATGGG CGATTTCTGG 
CGGATGGTGT GGGAACAGCG CACAGCCACT GTGGTCATGA TGACGCGGCT GGAGGAGAAG 
TCCCGGGTGA AATGTGACCA GTACTGGCCA GCTCGTGGCA CCGAGACCTA TGGCCTCATC 
CAGGTGACCC TGCTGGACAC GGTGGAGCTG GCCACCTACA CTGTGCGCAC CTTCGCGCTC 
TACAAGAGTG GCTCCAGTGA GAAGCGGGAG CTGCGTCAGT TCCAGTTCAT GGCCTGGCCA 
GACCACGGAG TCCCCGAGTA CCCGACCCCT ATCCTGGCCT TCCTGCGGCG GGTCAAGGCT 
TGCAACCCCC TGGACGCGGG GCCCATGGTG GTGCACTGCA GCGCGGGCGT GGGCCGCACG 
GGCTGCTTCA TCGTCATCGA CGCCATGCTG GAGCGGATGA AGCACGAGAA GACAGTGGAC 
ATCTACGGCC ACGTGACGTG CATGCGGGCG CAGCGCAACT ACATGGTGCA GACTGAGGAC 
CAGTACGTGT TCATCCACGA GGCGCTGCTG GAGGCCGCCA TGTGTGGACA CACGGAGGTG 
CCCGCCCGCA ACCTCTACGC CCACATCCAG AAGCTGGGCC AAGTGCCTCC CGGGGAGAGT 
GTGACTGCCA TGGAGCTGGA GTTCAAGTTG CTGGCCAACT CCAAGGCCCA CACATCCCGC 
TTCATCAGCG CCAACCTGCC CTGCAACAAG TTCAAGAACC GCCTGGTGAA CATCATGCCC 
TACGAACTGA CCCGTGTGTG TCTGCAGCCC ATCCGCGGCG TGGAGGGCTC CGACTACATC 
AATGCCAGCT TCCTGGATGG CTATCGACAG CAGAAGGCCT ACATAGCCAC ACAGGGGCCT 
CTGGCGGAGA GCACCGAGGA TTTCTGGCGC ATGTTATGGG AGCACAACTC CACCATCATC 
GTCATGCTGA CCAGGCTTCG GGAGATGGGC CGGGAGAAAT GCCACCAGTA CTGGCCTGCA 
GAGCGCTCTG CTCGCTACCA GTACTTTGTC GTCGACCCGA TGGCTGAGTA CAACATGCCC 
CAGTATATTC TCCGTGAGTT CAAGGTCACG GATGCCCGGG ATGGGCAGTC GAGGACAATC 
CGGCAGTTCC AGTTCACAGA CTGGCCGGAG CAGGGCGTGC CTAAAACGGG CGAGGGCTTC 
ATCGACTTCA TCGGGCAGGT GCACAAGACC AAGGAGCAGT TCGGGCAGGA CGGGCCCATC 
ACGGTGCACT GCAGTGCTGG TGTGGGCCGC ACTGGGGTGT TCATCACGCT GAGCATTGTC 
CTGGAGCGGA TGCGCTACGA AGGCGTGGTT GACATGTTCC AGACCGTGAA GACCCTGCGC 
ACACAGCGCC CAGCCATGGT GCAGACGGAG GACCAGTATC AGCTGTGCTA CCGCGCGGCC 
CTGGAGTACC TCGGAAGCTT TGACCACTAT GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843359.1
CDS	291..5129
Translation	MTPEPAPGRTMVPLVPALVMLGLVAGAHGDSKPVFVKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPLGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPAASNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLVVTETTATSVTLTWDSGNSEPVSYYGIQYRPAGAEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDSRRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEVDSDTRIQLSWLLPPQERIVKYELVYWAAEDEGQQHKVTFDPTSSYTVEDLKPDTLYRFQLAARSELGVGVFTPTIEARTAQSMPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGAPIIQDVMLAEAQWQPEESEDYETTISGLTPETTYSITVAAYTTKGDGARSKPKIVTTTGAVFAKNFRVEAAMKTSVLLSWEVPDSYKSAVPFRILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPLPASAYIEDGRFTLTMPRVQEPALVRWFYIMVVPIDRMGGSMLAPQWSTPEELELDELLEAIEQGGGERLRRRRQTERLKPYVAAQVDVLPETFTLGDKKNYQGFYNRPLSPDLSYQCFVLASLKEPVDQKRYACSPYSDEIVVQVTPAQQQEEPELLWVTGPVLAVILIVLIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSIDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETYGLIQVTLLDTVELATYTVRTFALYKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRAQRNYMVQTEDQYVFIHEALLEAAMCGHTEVPARNLYAHIQKLGQVPPGESVTAMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHNSTIIVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_024987591.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987591.1

ORF Insert Sequence:

ATGACCCCTG AGCCAGCCCC AGGGAGGACG ATGGTGCCCC TTGTCCCTGC ACTTGTGATG 
CTTGGTTTGG TGGCAGGTGC CCATGGCGAC AGCAAACCTG TCTTTGTCAA GGTCCCCGAG 
GACCAGACTG GATTGTCAGG AGGGGTGGCC TCCTTCGTGT GCCAAGCCAC GGGGGAACCC 
AAGCCGCGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTCGAGGTC 
ATCGAGTTTG ATGACGGGGC AGGGTCTGTG CTGCGGATCC AGCCACTGCG GGTGCAGCGA 
GACGAAGCCA TCTATGAGTG CACGGCCACC AACAGTCTGG GGGAGATCAA CACCAGTGCC 
AAGCTCTCAG TGCTCGAAGA GGAACAGCTG CCCCTTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCCCGC ACGGCCACTA TGCTGTGTGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC CGCCGCGAGC 
AACGGCCGCA TCAAGCAACT GCGTTCAGGT GCCTTGCAGA TTGAGAGCAG CGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCTGCAG GCACTCGTTA CTCGGCCCCT 
GCCAACCTAT ATGTGCGAGT GCGCCGCGTG GCTCCGCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGCGG CAGCGTGAAC CTGACGTGCG TCGCCGTTGG CGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAA GAGCTCACCA AGGAGGACGA GATGCCGGTC 
GGCCGCAATG TGCTGGAGCT CAGCAACGTG GTGCGCTCCG CCAACTACAC TTGCGTGGCC 
ATCTCCTCCC TGGGCATGAT CGAGGCCACG GCCCAGGTCA CCGTGAAAGC CCTGCCGAAG 
CCTCCAATTG ACCTTGTGGT GACAGAGACA ACTGCCACCA GCGTCACTCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT ATCCTACTAT GGCATCCAGT ACCGCCCAGC GGGCGCGGAG 
GGCCCCTTCC AGGAGGTGGA CGGCGTGGCC ACCACCCGCT ACAGCATCGG CGGCCTGAGC 
CCTTTCTCAG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGGCG CGGGCCGCCC 
AGCGAGGCGG TGCGGGCGCG CACGGGGGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTAAGCGC CAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCT 
AACGGCCTGG TGCGGGGCTA CCGCGTCTAC TACACCCCCG ACTCCCGCCG CCCTCTGAGC 
GCCTGGCACA AGCACAACAC AGACGCCGGG CTCCTCACCA CTGTGGGCAG CCTGCTGCCT 
GGCATCACCT ACAGCCTGCG TGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCCCCCAGC 
CCCACCATCC AGGTCAAGAC GCAGCAGGGA GTGCCTGCCC AGCCCGCGGA CTTCCAGGCC 
GAGGTGGACT CAGACACCAG GATCCAGCTC TCGTGGCTGC TGCCCCCGCA GGAGCGGATC 
GTCAAGTATG AGCTGGTGTA CTGGGCGGCA GAGGACGAGG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CGTCCTCCTA TACCGTAGAG GACCTGAAGC CCGACACACT GTACCGCTTC 
CAGCTGGCCG CCCGCTCTGA GCTGGGGGTG GGAGTCTTCA CCCCCACCAT TGAGGCTCGC 
ACAGCACAGT CCATGCCCAG TGGGCCACCG CGGAAGGTGG AGGTGGAGCC GCTCAACTCC 
ACTGCCGTGC GCGTCTCCTG GAAGCTGCCC GTCCCCAGCA AGCAGCATGG CCAGATCCGC 
GGCTACCAGG TCACCTACGT GCGGCTGGAG AATGGCGAGC CCCGCGGCGC CCCCATCATC 
CAGGACGTCA TGCTGGCCGA GGCCCAGTGG CAGCCAGAGG AGTCCGAGGA CTATGAAACC 
ACCATCAGCG GCCTGACCCC GGAGACCACC TACTCCATCA CCGTGGCTGC CTACACCACC 
AAGGGGGACG GTGCCCGCAG CAAGCCCAAA ATTGTCACGA CCACGGGGGC AGTGTTTGCC 
AAGAACTTCC GTGTGGAGGC TGCAATGAAG ACATCTGTGC TGCTCAGCTG GGAGGTGCCC 
GACTCCTACA AGTCAGCAGT GCCCTTCAGG ATCCTGTACA ATGGGCAGAG CGTGGAGGTG 
GACGGGCACT CCATGCGGAA GCTGATTGCA GACCTGCAGC CCAACACCGA ATACTCATTT 
GTGCTGATGA ACCGCGGCAG CAGCGCGGGG GGCCTGCAAC ACCTCGTGTC CATCCGCACG 
GCCCCCGACC TCCTGCCGCA CAAGCCACTG CCAGCCTCGG CCTACATCGA GGACGGCCGC 
TTCACCCTCA CCATGCCCCG CGTGCAGGAA CCCGCACTGG TCAGGTGGTT CTACATCATG 
GTGGTGCCCA TTGACCGCAT GGGAGGGAGC ATGCTGGCGC CACAGTGGAG CACGCCCGAG 
GAGCTGGAGC TGGACGAGCT GCTGGAGGCC ATCGAGCAGG GCGGCGGGGA GCGGCTGCGG 
CGCCGGCGGC AGACGGAGCG GCTGAAGCCA TACGTGGCCG CTCAGGTGGA CGTTCTCCCG 
GAGACCTTCA CCTTGGGGGA CAAGAAGAAC TATCAAGGCT TCTACAACCG GCCGCTGTCT 
CCAGACCTGA GCTACCAGTG CTTTGTGCTC GCCTCCCTGA AGGAACCCGT GGACCAGAAG 
CGCTATGCCT GCAGCCCCTA CTCCGATGAG ATCGTGGTCC AGGTGACACC AGCCCAGCAG 
CAGGAGGAAC CCGAGCTGCT GTGGGTGACG GGCCCCGTCC TGGCAGTCAT TCTCATCGTC 
CTCATTGTCA TCGCCATTCT CCTGTTCAAG AGGAAAAGGA CCCACTCCCC ATCGTCCAAG 
GATGAGCAGT CAATTGGGCT GAAGGACTCC TTGCTGGCCC ACTCCTCCGA CCCTGTGGAG 
ATGCGGCGGC TCAACTACCA GACCCCAGGT ATGCGAGACC ACCCGCCCAT CCCCATCACC 
GACCTAGCTG ACAACATCGA GCGCCTCAAA GCCAATGACG GCCTCAAGTT CTCCCAGGAG 
TACGAGTCAA TTGACCCAGG ACAGCAGTTC ACGTGGGAGA ATTCAAACCT GGAGGTGAAC 
AAGCCGAAGA ACCGCTATGC CAACGTCATT GCTTACGACC ACTCTCGAGT CATCCTTACC 
TCCATCGATG GTGTCCCCGG GAGCGACTAC ATCAATGCCA ATTACATCGA CGGCTACCGC 
AAGCAGAACG CCTACATTGC CACGCAGGGC CCGCTGCCTG AGACCATGGG CGATTTCTGG 
CGGATGGTGT GGGAACAGCG CACAGCCACT GTGGTCATGA TGACGCGGCT GGAGGAGAAG 
TCCCGGGTGA AATGTGACCA GTACTGGCCA GCTCGTGGCA CCGAGACCTA TGGCCTCATC 
CAGGTGACCC TGCTGGACAC GGTGGAGCTG GCCACCTACA CTGTGCGCAC CTTCGCGCTC 
TACAAGAGTG GCTCCAGTGA GAAGCGGGAG CTGCGTCAGT TCCAGTTCAT GGCCTGGCCA 
GACCACGGAG TCCCCGAGTA CCCGACCCCT ATCCTGGCCT TCCTGCGGCG GGTCAAGGCT 
TGCAACCCCC TGGACGCGGG GCCCATGGTG GTGCACTGCA GCGCGGGCGT GGGCCGCACG 
GGCTGCTTCA TCGTCATCGA CGCCATGCTG GAGCGGATGA AGCACGAGAA GACAGTGGAC 
ATCTACGGCC ACGTGACGTG CATGCGGGCG CAGCGCAACT ACATGGTGCA GACTGAGGAC 
CAGTACGTGT TCATCCACGA GGCGCTGCTG GAGGCCGCCA TGTGTGGACA CACGGAGGTG 
CCCGCCCGCA ACCTCTACGC CCACATCCAG AAGCTGGGCC AAGTGCCTCC CGGGGAGAGT 
GTGACTGCCA TGGAGCTGGA GTTCAAGTTG CTGGCCAACT CCAAGGCCCA CACATCCCGC 
TTCATCAGCG CCAACCTGCC CTGCAACAAG TTCAAGAACC GCCTGGTGAA CATCATGCCC 
TACGAACTGA CCCGTGTGTG TCTGCAGCCC ATCCGCGGCG TGGAGGGCTC CGACTACATC 
AATGCCAGCT TCCTGGATGG CTATCGACAG CAGAAGGCCT ACATAGCCAC ACAGGGGCCT 
CTGGCGGAGA GCACCGAGGA TTTCTGGCGC ATGTTATGGG AGCACAACTC CACCATCATC 
GTCATGCTGA CCAGGCTTCG GGAGATGGGC CGGGAGAAAT GCCACCAGTA CTGGCCTGCA 
GAGCGCTCTG CTCGCTACCA GTACTTTGTC GTCGACCCGA TGGCTGAGTA CAACATGCCC 
CAGTATATTC TCCGTGAGTT CAAGGTCACG GATGCCCGGG ATGGGCAGTC GAGGACAATC 
CGGCAGTTCC AGTTCACAGA CTGGCCGGAG CAGGGCGTGC CTAAAACGGG CGAGGGCTTC 
ATCGACTTCA TCGGGCAGGT GCACAAGACC AAGGAGCAGT TCGGGCAGGA CGGGCCCATC 
ACGGTGCACT GCAGTGCTGG TGTGGGCCGC ACTGGGGTGT TCATCACGCT GAGCATTGTC 
CTGGAGCGGA TGCGCTACGA AGGCGTGGTT GACATGTTCC AGACCGTGAA GACCCTGCGC 
ACACAGCGCC CAGCCATGGT GCAGACGGAG GACCAGTATC AGCTGTGCTA CCGCGCGGCC 
CTGGAGTACC TCGGAAGCTT TGACCACTAT GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_024987593.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843361.1 Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X7, mRNA.

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CloneID	OBa232666
Clone ID Related Accession (Same CDS sequence)	XM_024987593.1
Accession Version	XM_024987593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	receptor-type tyrosine-protein phosphatase F isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCTG AGCCAGCCCC AGGGAGGACG ATGGTGCCCC TTGTCCCTGC ACTTGTGATG 
CTTGGTTTGG TGGCAGGTGC CCATGGCGAC AGCAAACCTG TCTTTGTCAA GGTCCCCGAG 
GACCAGACTG GATTGTCAGG AGGGGTGGCC TCCTTCGTGT GCCAAGCCAC GGGGGAACCC 
AAGCCGCGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTCGAGGTC 
ATCGAGTTTG ATGACGGGGC AGGGTCTGTG CTGCGGATCC AGCCACTGCG GGTGCAGCGA 
GACGAAGCCA TCTATGAGTG CACGGCCACC AACAGTCTGG GGGAGATCAA CACCAGTGCC 
AAGCTCTCAG TGCTCGAAGA GGAACAGCTG CCCCTTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCCCGC ACGGCCACTA TGCTGTGTGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC CGCCGCGAGC 
AACGGCCGCA TCAAGCAACT GCGTTCAGGT GCCTTGCAGA TTGAGAGCAG CGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCTGCAG GCACTCGTTA CTCGGCCCCT 
GCCAACCTAT ATGTGCGAGT GCGCCGCGTG GCTCCGCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGCGG CAGCGTGAAC CTGACGTGCG TCGCCGTTGG CGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAA GAGCTCACCA AGGAGGACGA GATGCCGGTC 
GGCCGCAATG TGCTGGAGCT CAGCAACGTG GTGCGCTCCG CCAACTACAC TTGCGTGGCC 
ATCTCCTCCC TGGGCATGAT CGAGGCCACG GCCCAGGTCA CCGTGAAAGC CCTGCCGAAG 
CCTCCAATTG ACCTTGTGGT GACAGAGACA ACTGCCACCA GCGTCACTCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT ATCCTACTAT GGCATCCAGT ACCGCCCAGC GGGCGCGGAG 
GGCCCCTTCC AGGAGGTGGA CGGCGTGGCC ACCACCCGCT ACAGCATCGG CGGCCTGAGC 
CCTTTCTCAG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGGCG CGGGCCGCCC 
AGCGAGGCGG TGCGGGCGCG CACGGGGGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTAAGCGC CAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCT 
AACGGCCTGG TGCGGGGCTA CCGCGTCTAC TACACCCCCG ACTCCCGCCG CCCTCTGAGC 
GCCTGGCACA AGCACAACAC AGACGCCGGG CTCCTCACCA CTGTGGGCAG CCTGCTGCCT 
GGCATCACCT ACAGCCTGCG TGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCCCCCAGC 
CCCACCATCC AGGTCAAGAC GCAGCAGGGA GTGCCTGCCC AGCCCGCGGA CTTCCAGGCC 
GAGGTGGACT CAGACACCAG GATCCAGCTC TCGTGGCTGC TGCCCCCGCA GGAGCGGATC 
GTCAAGTATG AGCTGGTGTA CTGGGCGGCA GAGGACGAGG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CGTCCTCCTA TACCGTAGAG GACCTGAAGC CCGACACACT GTACCGCTTC 
CAGCTGGCCG CCCGCTCTGA GCTGGGGGTG GGAGTCTTCA CCCCCACCAT TGAGGCTCGC 
ACAGCACAGT CCATGCCCAG TGGGCCACCG CGGAAGGTGG AGGTGGAGCC GCTCAACTCC 
ACTGCCGTGC GCGTCTCCTG GAAGCTGCCC GTCCCCAGCA AGCAGCATGG CCAGATCCGC 
GGCTACCAGG TCACCTACGT GCGGCTGGAG AATGGCGAGC CCCGCGGCGC CCCCATCATC 
CAGGACGTCA TGCTGGCCGA GGCCCAGGAA ACCACCATCA GCGGCCTGAC CCCGGAGACC 
ACCTACTCCA TCACCGTGGC TGCCTACACC ACCAAGGGGG ACGGTGCCCG CAGCAAGCCC 
AAAATTGTCA CGACCACGGG GGCAGTGTTT GCCAAGAACT TCCGTGTGGA GGCTGCAATG 
AAGACATCTG TGCTGCTCAG CTGGGAGGTG CCCGACTCCT ACAAGTCAGC AGTGCCCTTC 
AGGATCCTGT ACAATGGGCA GAGCGTGGAG GTGGACGGGC ACTCCATGCG GAAGCTGATT 
GCAGACCTGC AGCCCAACAC CGAATACTCA TTTGTGCTGA TGAACCGCGG CAGCAGCGCG 
GGGGGCCTGC AACACCTCGT GTCCATCCGC ACGGCCCCCG ACCTCCTGCC GCACAAGCCA 
CTGCCAGCCT CGGCCTACAT CGAGGACGGC CGCTTCACCC TCACCATGCC CCGCGTGCAG 
GAACCCGCAC TGGTCAGGTG GTTCTACATC ATGGTGGTGC CCATTGACCG CATGGGAGGG 
AGCATGCTGG CGCCACAGTG GAGCACGCCC GAGGAGCTGG AGCTGGACGA GCTGCTGGAG 
GCCATCGAGC AGGGCGGCGG GGAGCGGCTG CGGCGCCGGC GGCAGACGGA GCGGCTGAAG 
CCATACGTGG CCGCTCAGGT GGACGTTCTC CCGGAGACCT TCACCTTGGG GGACAAGAAG 
AACTATCAAG GCTTCTACAA CCGGCCGCTG TCTCCAGACC TGAGCTACCA GTGCTTTGTG 
CTCGCCTCCC TGAAGGAACC CGTGGACCAG AAGCGCTATG CCTGCAGCCC CTACTCCGAT 
GAGATCGTGG TCCAGGTGAC ACCAGCCCAG CAGCAGGAGG AACCCGAGCT GCTGTGGGTG 
ACGGGCCCCG TCCTGGCAGT CATTCTCATC GTCCTCATTG TCATCGCCAT TCTCCTGTTC 
AAGAGGAAAA GGACCCACTC CCCATCGTCC AAGGATGAGC AGTCAATTGG GCTGAAGGAC 
TCCTTGCTGG CCCACTCCTC CGACCCTGTG GAGATGCGGC GGCTCAACTA CCAGACCCCA 
GGTATGCGAG ACCACCCGCC CATCCCCATC ACCGACCTAG CTGACAACAT CGAGCGCCTC 
AAAGCCAATG ACGGCCTCAA GTTCTCCCAG GAGTACGAGT CAATTGACCC AGGACAGCAG 
TTCACGTGGG AGAATTCAAA CCTGGAGGTG AACAAGCCGA AGAACCGCTA TGCCAACGTC 
ATTGCTTACG ACCACTCTCG AGTCATCCTT ACCTCCATCG ATGGTGTCCC CGGGAGCGAC 
TACATCAATG CCAATTACAT CGACGGCTAC CGCAAGCAGA ACGCCTACAT TGCCACGCAG 
GGCCCGCTGC CTGAGACCAT GGGCGATTTC TGGCGGATGG TGTGGGAACA GCGCACAGCC 
ACTGTGGTCA TGATGACGCG GCTGGAGGAG AAGTCCCGGG TGAAATGTGA CCAGTACTGG 
CCAGCTCGTG GCACCGAGAC CTATGGCCTC ATCCAGGTGA CCCTGCTGGA CACGGTGGAG 
CTGGCCACCT ACACTGTGCG CACCTTCGCG CTCTACAAGA GTGGCTCCAG TGAGAAGCGG 
GAGCTGCGTC AGTTCCAGTT CATGGCCTGG CCAGACCACG GAGTCCCCGA GTACCCGACC 
CCTATCCTGG CCTTCCTGCG GCGGGTCAAG GCTTGCAACC CCCTGGACGC GGGGCCCATG 
GTGGTGCACT GCAGCGCGGG CGTGGGCCGC ACGGGCTGCT TCATCGTCAT CGACGCCATG 
CTGGAGCGGA TGAAGCACGA GAAGACAGTG GACATCTACG GCCACGTGAC GTGCATGCGG 
GCGCAGCGCA ACTACATGGT GCAGACTGAG GACCAGTACG TGTTCATCCA CGAGGCGCTG 
CTGGAGGCCG CCATGTGTGG ACACACGGAG GTGCCCGCCC GCAACCTCTA CGCCCACATC 
CAGAAGCTGG GCCAAGTGCC TCCCGGGGAG AGTGTGACTG CCATGGAGCT GGAGTTCAAG 
TTGCTGGCCA ACTCCAAGGC CCACACATCC CGCTTCATCA GCGCCAACCT GCCCTGCAAC 
AAGTTCAAGA ACCGCCTGGT GAACATCATG CCCTACGAAC TGACCCGTGT GTGTCTGCAG 
CCCATCCGCG GCGTGGAGGG CTCCGACTAC ATCAATGCCA GCTTCCTGGA TGGCTATCGA 
CAGCAGAAGG CCTACATAGC CACACAGGGG CCTCTGGCGG AGAGCACCGA GGATTTCTGG 
CGCATGTTAT GGGAGCACAA CTCCACCATC ATCGTCATGC TGACCAGGCT TCGGGAGATG 
GGCCGGGAGA AATGCCACCA GTACTGGCCT GCAGAGCGCT CTGCTCGCTA CCAGTACTTT 
GTCGTCGACC CGATGGCTGA GTACAACATG CCCCAGTATA TTCTCCGTGA GTTCAAGGTC 
ACGGATGCCC GGGATGGGCA GTCGAGGACA ATCCGGCAGT TCCAGTTCAC AGACTGGCCG 
GAGCAGGGCG TGCCTAAAAC GGGCGAGGGC TTCATCGACT TCATCGGGCA GGTGCACAAG 
ACCAAGGAGC AGTTCGGGCA GGACGGGCCC ATCACGGTGC ACTGCAGTGC TGGTGTGGGC 
CGCACTGGGG TGTTCATCAC GCTGAGCATT GTCCTGGAGC GGATGCGCTA CGAAGGCGTG 
GTTGACATGT TCCAGACCGT GAAGACCCTG CGCACACAGC GCCCAGCCAT GGTGCAGACG 
GAGGACCAGT ATCAGCTGTG CTACCGCGCG GCCCTGGAGT ACCTCGGAAG CTTTGACCAC 
TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843361.1
CDS	291..5102
Translation	MTPEPAPGRTMVPLVPALVMLGLVAGAHGDSKPVFVKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPLGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPAASNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLVVTETTATSVTLTWDSGNSEPVSYYGIQYRPAGAEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDSRRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEVDSDTRIQLSWLLPPQERIVKYELVYWAAEDEGQQHKVTFDPTSSYTVEDLKPDTLYRFQLAARSELGVGVFTPTIEARTAQSMPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGAPIIQDVMLAEAQETTISGLTPETTYSITVAAYTTKGDGARSKPKIVTTTGAVFAKNFRVEAAMKTSVLLSWEVPDSYKSAVPFRILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPLPASAYIEDGRFTLTMPRVQEPALVRWFYIMVVPIDRMGGSMLAPQWSTPEELELDELLEAIEQGGGERLRRRRQTERLKPYVAAQVDVLPETFTLGDKKNYQGFYNRPLSPDLSYQCFVLASLKEPVDQKRYACSPYSDEIVVQVTPAQQQEEPELLWVTGPVLAVILIVLIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSIDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETYGLIQVTLLDTVELATYTVRTFALYKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRAQRNYMVQTEDQYVFIHEALLEAAMCGHTEVPARNLYAHIQKLGQVPPGESVTAMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHNSTIIVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_024987593.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987593.1

ORF Insert Sequence:

ATGACCCCTG AGCCAGCCCC AGGGAGGACG ATGGTGCCCC TTGTCCCTGC ACTTGTGATG 
CTTGGTTTGG TGGCAGGTGC CCATGGCGAC AGCAAACCTG TCTTTGTCAA GGTCCCCGAG 
GACCAGACTG GATTGTCAGG AGGGGTGGCC TCCTTCGTGT GCCAAGCCAC GGGGGAACCC 
AAGCCGCGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTCGAGGTC 
ATCGAGTTTG ATGACGGGGC AGGGTCTGTG CTGCGGATCC AGCCACTGCG GGTGCAGCGA 
GACGAAGCCA TCTATGAGTG CACGGCCACC AACAGTCTGG GGGAGATCAA CACCAGTGCC 
AAGCTCTCAG TGCTCGAAGA GGAACAGCTG CCCCTTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCCCGC ACGGCCACTA TGCTGTGTGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC CGCCGCGAGC 
AACGGCCGCA TCAAGCAACT GCGTTCAGGT GCCTTGCAGA TTGAGAGCAG CGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCTGCAG GCACTCGTTA CTCGGCCCCT 
GCCAACCTAT ATGTGCGAGT GCGCCGCGTG GCTCCGCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGCGG CAGCGTGAAC CTGACGTGCG TCGCCGTTGG CGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAA GAGCTCACCA AGGAGGACGA GATGCCGGTC 
GGCCGCAATG TGCTGGAGCT CAGCAACGTG GTGCGCTCCG CCAACTACAC TTGCGTGGCC 
ATCTCCTCCC TGGGCATGAT CGAGGCCACG GCCCAGGTCA CCGTGAAAGC CCTGCCGAAG 
CCTCCAATTG ACCTTGTGGT GACAGAGACA ACTGCCACCA GCGTCACTCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT ATCCTACTAT GGCATCCAGT ACCGCCCAGC GGGCGCGGAG 
GGCCCCTTCC AGGAGGTGGA CGGCGTGGCC ACCACCCGCT ACAGCATCGG CGGCCTGAGC 
CCTTTCTCAG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGGCG CGGGCCGCCC 
AGCGAGGCGG TGCGGGCGCG CACGGGGGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTAAGCGC CAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCT 
AACGGCCTGG TGCGGGGCTA CCGCGTCTAC TACACCCCCG ACTCCCGCCG CCCTCTGAGC 
GCCTGGCACA AGCACAACAC AGACGCCGGG CTCCTCACCA CTGTGGGCAG CCTGCTGCCT 
GGCATCACCT ACAGCCTGCG TGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCCCCCAGC 
CCCACCATCC AGGTCAAGAC GCAGCAGGGA GTGCCTGCCC AGCCCGCGGA CTTCCAGGCC 
GAGGTGGACT CAGACACCAG GATCCAGCTC TCGTGGCTGC TGCCCCCGCA GGAGCGGATC 
GTCAAGTATG AGCTGGTGTA CTGGGCGGCA GAGGACGAGG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CGTCCTCCTA TACCGTAGAG GACCTGAAGC CCGACACACT GTACCGCTTC 
CAGCTGGCCG CCCGCTCTGA GCTGGGGGTG GGAGTCTTCA CCCCCACCAT TGAGGCTCGC 
ACAGCACAGT CCATGCCCAG TGGGCCACCG CGGAAGGTGG AGGTGGAGCC GCTCAACTCC 
ACTGCCGTGC GCGTCTCCTG GAAGCTGCCC GTCCCCAGCA AGCAGCATGG CCAGATCCGC 
GGCTACCAGG TCACCTACGT GCGGCTGGAG AATGGCGAGC CCCGCGGCGC CCCCATCATC 
CAGGACGTCA TGCTGGCCGA GGCCCAGGAA ACCACCATCA GCGGCCTGAC CCCGGAGACC 
ACCTACTCCA TCACCGTGGC TGCCTACACC ACCAAGGGGG ACGGTGCCCG CAGCAAGCCC 
AAAATTGTCA CGACCACGGG GGCAGTGTTT GCCAAGAACT TCCGTGTGGA GGCTGCAATG 
AAGACATCTG TGCTGCTCAG CTGGGAGGTG CCCGACTCCT ACAAGTCAGC AGTGCCCTTC 
AGGATCCTGT ACAATGGGCA GAGCGTGGAG GTGGACGGGC ACTCCATGCG GAAGCTGATT 
GCAGACCTGC AGCCCAACAC CGAATACTCA TTTGTGCTGA TGAACCGCGG CAGCAGCGCG 
GGGGGCCTGC AACACCTCGT GTCCATCCGC ACGGCCCCCG ACCTCCTGCC GCACAAGCCA 
CTGCCAGCCT CGGCCTACAT CGAGGACGGC CGCTTCACCC TCACCATGCC CCGCGTGCAG 
GAACCCGCAC TGGTCAGGTG GTTCTACATC ATGGTGGTGC CCATTGACCG CATGGGAGGG 
AGCATGCTGG CGCCACAGTG GAGCACGCCC GAGGAGCTGG AGCTGGACGA GCTGCTGGAG 
GCCATCGAGC AGGGCGGCGG GGAGCGGCTG CGGCGCCGGC GGCAGACGGA GCGGCTGAAG 
CCATACGTGG CCGCTCAGGT GGACGTTCTC CCGGAGACCT TCACCTTGGG GGACAAGAAG 
AACTATCAAG GCTTCTACAA CCGGCCGCTG TCTCCAGACC TGAGCTACCA GTGCTTTGTG 
CTCGCCTCCC TGAAGGAACC CGTGGACCAG AAGCGCTATG CCTGCAGCCC CTACTCCGAT 
GAGATCGTGG TCCAGGTGAC ACCAGCCCAG CAGCAGGAGG AACCCGAGCT GCTGTGGGTG 
ACGGGCCCCG TCCTGGCAGT CATTCTCATC GTCCTCATTG TCATCGCCAT TCTCCTGTTC 
AAGAGGAAAA GGACCCACTC CCCATCGTCC AAGGATGAGC AGTCAATTGG GCTGAAGGAC 
TCCTTGCTGG CCCACTCCTC CGACCCTGTG GAGATGCGGC GGCTCAACTA CCAGACCCCA 
GGTATGCGAG ACCACCCGCC CATCCCCATC ACCGACCTAG CTGACAACAT CGAGCGCCTC 
AAAGCCAATG ACGGCCTCAA GTTCTCCCAG GAGTACGAGT CAATTGACCC AGGACAGCAG 
TTCACGTGGG AGAATTCAAA CCTGGAGGTG AACAAGCCGA AGAACCGCTA TGCCAACGTC 
ATTGCTTACG ACCACTCTCG AGTCATCCTT ACCTCCATCG ATGGTGTCCC CGGGAGCGAC 
TACATCAATG CCAATTACAT CGACGGCTAC CGCAAGCAGA ACGCCTACAT TGCCACGCAG 
GGCCCGCTGC CTGAGACCAT GGGCGATTTC TGGCGGATGG TGTGGGAACA GCGCACAGCC 
ACTGTGGTCA TGATGACGCG GCTGGAGGAG AAGTCCCGGG TGAAATGTGA CCAGTACTGG 
CCAGCTCGTG GCACCGAGAC CTATGGCCTC ATCCAGGTGA CCCTGCTGGA CACGGTGGAG 
CTGGCCACCT ACACTGTGCG CACCTTCGCG CTCTACAAGA GTGGCTCCAG TGAGAAGCGG 
GAGCTGCGTC AGTTCCAGTT CATGGCCTGG CCAGACCACG GAGTCCCCGA GTACCCGACC 
CCTATCCTGG CCTTCCTGCG GCGGGTCAAG GCTTGCAACC CCCTGGACGC GGGGCCCATG 
GTGGTGCACT GCAGCGCGGG CGTGGGCCGC ACGGGCTGCT TCATCGTCAT CGACGCCATG 
CTGGAGCGGA TGAAGCACGA GAAGACAGTG GACATCTACG GCCACGTGAC GTGCATGCGG 
GCGCAGCGCA ACTACATGGT GCAGACTGAG GACCAGTACG TGTTCATCCA CGAGGCGCTG 
CTGGAGGCCG CCATGTGTGG ACACACGGAG GTGCCCGCCC GCAACCTCTA CGCCCACATC 
CAGAAGCTGG GCCAAGTGCC TCCCGGGGAG AGTGTGACTG CCATGGAGCT GGAGTTCAAG 
TTGCTGGCCA ACTCCAAGGC CCACACATCC CGCTTCATCA GCGCCAACCT GCCCTGCAAC 
AAGTTCAAGA ACCGCCTGGT GAACATCATG CCCTACGAAC TGACCCGTGT GTGTCTGCAG 
CCCATCCGCG GCGTGGAGGG CTCCGACTAC ATCAATGCCA GCTTCCTGGA TGGCTATCGA 
CAGCAGAAGG CCTACATAGC CACACAGGGG CCTCTGGCGG AGAGCACCGA GGATTTCTGG 
CGCATGTTAT GGGAGCACAA CTCCACCATC ATCGTCATGC TGACCAGGCT TCGGGAGATG 
GGCCGGGAGA AATGCCACCA GTACTGGCCT GCAGAGCGCT CTGCTCGCTA CCAGTACTTT 
GTCGTCGACC CGATGGCTGA GTACAACATG CCCCAGTATA TTCTCCGTGA GTTCAAGGTC 
ACGGATGCCC GGGATGGGCA GTCGAGGACA ATCCGGCAGT TCCAGTTCAC AGACTGGCCG 
GAGCAGGGCG TGCCTAAAAC GGGCGAGGGC TTCATCGACT TCATCGGGCA GGTGCACAAG 
ACCAAGGAGC AGTTCGGGCA GGACGGGCCC ATCACGGTGC ACTGCAGTGC TGGTGTGGGC 
CGCACTGGGG TGTTCATCAC GCTGAGCATT GTCCTGGAGC GGATGCGCTA CGAAGGCGTG 
GTTGACATGT TCCAGACCGT GAAGACCCTG CGCACACAGC GCCCAGCCAT GGTGCAGACG 
GAGGACCAGT ATCAGCTGTG CTACCGCGCG GCCCTGGAGT ACCTCGGAAG CTTTGACCAC 
TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( NM_001101079.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001094549.1 Bos taurus protein tyrosine phosphatase receptor type F (PTPRF), mRNA.

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CloneID	OBa44324
Clone ID Related Accession (Same CDS sequence)	NM_001101079.1
Accession Version	NM_001101079.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	receptor-type tyrosine-protein phosphatase F precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC151592.1. On or before Aug 14, 2007 this sequence version replaced XM_589513.3, XM_001250802.1. ##Evidence-Data-START## Transcript exon combination :: BC151592.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGACCCCTG AGCCAGCCCC AGGGAGGACG ATGGTGCCCC TTGTCCCTGC ACTTGTGATG 
CTTGGTTTGG TGGCAGGTGC CCATGGCGAC AGCAAACCTG TCTTTGTCAA GGTCCCCGAG 
GACCAGACTG GATTGTCAGG AGGGGTGGCC TCCTTCGTGT GCCAAGCCAC GGGGGAACCC 
AAGCCGCGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTCGAGGTC 
ATCGAGTTTG ATGACGGGGC AGGGTCTGTG CTGCGGATCC AGCCACTGCG GGTGCAGCGA 
GACGAAGCCA TCTATGAGTG CACGGCCACC AACAGTCTGG GGGAGATCAA CACCAGTGCC 
AAGCTCTCAG TGCTCGAAGA GGAACAGCTG CCCCTTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCCCGC ACGGCCACTA TGCTGTGTGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC CGCCGCGAGC 
AACGGCCGCA TCAAGCAACT GCGTTCAGGT GCCTTGCAGA TTGAGAGCAG CGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCTGCAG GCACTCGTTA CTCGGCCCCT 
GCCAACCTAT ATGTGCGAGT GCGCCGCGTG GCTCCGCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGCGG CAGCGTGAAC CTGACGTGCG TCGCCGTTGG CGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAA GAGCTCACCA AGGAGGACGA GATGCCGGTC 
GGCCGCAATG TGCTGGAGCT CAGCAACGTG GTGCGCTCCG CCAACTACAC TTGCGTGGCC 
ATCTCCTCCC TGGGCATGAT CGAGGCCACG GCCCAGGTCA CCGTGAAAGC CCTGCCGAAG 
CCTCCAATTG ACCTTGTGGT GACAGAGACA ACTGCCACCA GCGTCACTCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT ATCCTACTAT GGCATCCAGT ACCGCCCAGC GGGCGCGGAG 
GGCCCCTTCC AGGAGGTGGA CGGCGTGGCC ACCACCCGCT ACAGCATCGG CGGCCTGAGC 
CCTTTCTCAG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGGCG CGGGCCGCCC 
AGCGAGGCGG TGCGGGCGCG CACGGGGGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTAAGCGC CAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCT 
AACGGCCTGG TGCGGGGCTA CCGCGTCTAC TACACCCCCG ACTCCCGCCG CCCTCTGAGC 
GCCTGGCACA AGCACAACAC AGACGCCGGG CTCCTCACCA CTGTGGGCAG CCTGCTGCCT 
GGCATCACCT ACAGCCTGCG TGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCCCCCAGC 
CCCACCATCC AGGTCAAGAC GCAGCAGGGA GTGCCTGCCC AGCCCGCGGA CTTCCAGGCC 
GAGGTGGACT CAGACACCAG GATCCAGCTC TCGTGGCTGC TGCCCCCGCA GGAGCGGATC 
GTCAAGTATG AGCTGGTGTA CTGGGCGGCA GAGGACGAGG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CGTCCTCCTA TACCGTAGAG GACCTGAAGC CCGACACACT GTACCGCTTC 
CAGCTGGCCG CCCGCTCTGA GCTGGGGGTG GGAGTCTTCA CCCCCACCAT TGAGGCTCGC 
ACAGCACAGT CCACCCCCTC CGCACCCCCC CAGAAGGTGA CGTGTGTGAG CGTGGGCTCC 
ACCACCGTCC GGGTAAGTTG GGTCCCGCCG CCGGCCGACA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCGGTGGAC GGCGAGGACC GTGGGCGGCA CGTGGTGGAC 
GGCATCGGCC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACGGACGTG GGCCCCGGCC CCGAGAGCAG CCCGGTGCTG 
GTCCGCACCG ACGAGGACGT GCCCAGTGGG CCACCGCGGA AGGTGGAGGT GGAGCCGCTC 
AACTCCACTG CCGTGCGCGT CTCCTGGAAG CTGCCCGTCC CCAGCAAGCA GCATGGCCAG 
ATCCGCGGCT ACCAGGTCAC CTACGTGCGG CTGGAGAATG GCGAGCCCCG CGGCGCCCCC 
ATCATCCAGG ACGTCATGCT GGCCGAGGCC CAGGAAACCA CCATCAGCGG CCTGACCCCG 
GAGACCACCT ACTCCATCAC CGTGGCTGCC TACACCACCA AGGGGGACGG TGCCCGCAGC 
AAGCCCAAAA TTGTCACGAC CACAGGGGCA GTCCCAGGAC GGCCCACCAT GATGGTCAGC 
ACCACGGCCA TGAACACGGC GCTGCTCCAG TGGCACCCGC CCAAGGAGCT GCCCGGGGAG 
CTCCTGGGCT ACCGGCTGCA GTACCGCCGA GCCGACGAGG CGCGGCCCAG CACCATCGAT 
TTTGGCAAGG ACGACCAGCA CTTTACGGTC ACTGGCCTGC ACAAAGGGGC CACCTACATC 
TTCCGGCTCA CTGCCAAGAA CCGGGCCGGC CTGGGCGAGG AGTTCGAGAA GGAAATCACG 
ACCCCCGAGG ACGTGCCCAG CGGTTTCCCC CAAAACCTGC GCGTGATAGG GCTGACCACA 
TCTACCACGG AACTGATCTG GGACCCCCCA GTGCTGGCGG AGAGGAACGG GCGCATCACC 
AACTACACCG TGGTGTACCG TGACATCAAC AGCCAGCAGG AGCTTCAGAA CGTCACTGCC 
GACACCCACC TGACGCTCTC CGGCCTCAAG CCAGACACCA CTTACGACAT CAAGGTCCGC 
GCACGCACCA GCAAGGGCGC CGGCCCGCTC AGCCCCAGCA TCCAGTCCCG GACCATGCCC 
GTGGAGCAAG TGTTTGCCAA GAACTTCCGT GTGGAGGCTG CAATGAAGAC ATCTGTGCTG 
CTCAGCTGGG AGGTGCCCGA CTCCTACAAG TCAGCAGTGC CCTTCAGGAT CCTGTACAAT 
GGGCAGAGCG TGGAGGTGGA CGGGCACTCC ATGCGGAAGC TGATTGCAGA CCTGCAGCCC 
AACACTGAAT ACTCATTTGT GCTGATGAAC CGCGGCAGCA GCGCGGGGGG CCTGCAACAC 
CTCGTGTCCA TCCGCACGGC CCCCGACCTC CTGCCGCACA AGCCACTGCC AGCCTCGGCC 
TACATCGAGG ACGGCCGCTT CACCCTCACC ATGCCCCGCG TGCAGGAACC CGCACTGGTC 
AGGTGGTTCT ACATCATGGT GGTGCCCATT GACCGCATGG GAGGGAGCAT GCTGGCGCCA 
CAGTGGAGCA CGCCCGAGGA GCTGGAGCTG GACGAGCTGC TGGAGGCCAT CGAGCAGGGC 
GGCGGGGAGC GGCTGCGGCG CCGGCGGCAG ACGGAGCGGC TGAAGCCATA CGTGGCCGCT 
CAGGTGGACG TTCTCCCGGA GACCTTCACC TTGGGGGACA AGAAGAACTA TCAAGGCTTC 
TACAACCGGC CGCTGTCTCC AGACCTGAGC TACCAGTGCT TTGTGCTCGC CTCCCTGAAG 
GAACCCGTGG ACCAGAAGCG CTATGCCTGC AGCCCCTACT CCGATGAGAT CGTGGTCCAG 
GTGACACCAG CCCAGCAGCA GGAGGAACCC GAGCTGCTGT GGGTGACGGG CCCCGTCCTG 
GCAGTCATTC TCATCGTCCT CATTGTCATC GCCATTCTCC TGTTCAAGAG GAAAAGGACC 
CACTCCCCAT CGTCCAAGGA TGAGCAGTCA ATTGGGCTGA AGGACTCCTT GCTGGCCCAC 
TCCTCCGACC CTGTGGAGAT GCGGCGGCTC AACTACCAGA CCCCAGGTAT GCGAGACCAC 
CCGCCCATCC CCATCACCGA CCTAGCTGAC AACATCGAGC GCCTCAAAGC CAATGACGGC 
CTCAAGTTCT CCCAGGAGTA CGAGTCAATT GACCCAGGAC AGCAGTTCAC GTGGGAGAAT 
TCAAACCTGG AGGTGAACAA GCCGAAGAAC CGCTATGCCA ACGTCATTGC TTACGACCAC 
TCTCGAGTCA TCCTTACCTC CATCGATGGT GTCCCCGGGA GCGACTACAT CAATGCCAAT 
TACATCGACG GCTACCGCAA GCAGAACGCC TACATTGCCA CGCAGGGCCC GCTGCCTGAG 
ACCATGGGCG ATTTCTGGCG GATGGTGTGG GAACAGCGCA CAGCCACTGT GGTCATGATG 
ACGCGGCTGG AGGAGAAGTC CCGGGTGAAA TGTGACCAGT ACTGGCCAGC TCGTGGCACC 
GAGACCTATG GCCTCATCCA GGTGACCCTG CTGGACACGG TGGAGCTGGC CACCTACACT 
GTGCGCACCT TCGCGCTCTA CAAGAGTGGC TCCAGTGAGA AGCGGGAGCT GCGTCAGTTC 
CAGTTCATGG CCTGGCCAGA CCACGGAGTC CCCGAGTACC CGACCCCTAT CCTGGCCTTC 
CTGCGGCGGG TCAAGGCTTG CAACCCCCTG GACGCGGGGC CCATGGTGGT GCACTGCAGC 
GCGGGCGTGG GCCGCACGGG CTGCTTCATC GTCATCGACG CCATGCTGGA GCGGATGAAG 
CACGAGAAGA CAGTGGACAT CTACGGCCAC GTGACGTGCA TGCGGGCGCA GCGCAACTAC 
ATGGTGCAGA CTGAGGACCA GTACGTGTTC ATCCACGAGG CGCTGCTGGA GGCCGCCATG 
TGTGGACACA CGGAGGTGCC CGCCCGCAAC CTCTACGCCC ACATCCAGAA GCTGGGCCAA 
GTGCCTCCCG GGGAGAGTGT GACTGCCATG GAGCTGGAGT TCAAGTTGCT GGCCAACTCC 
AAGGCCCACA CATCCCGCTT CATCAGCGCC AACCTGCCCT GCAACAAGTT CAAGAACCGC 
CTGGTGAACA TCATGCCCTA CGAACTGACC CGTGTGTGTC TGCAGCCCAT CCGCGGCGTG 
GAGGGCTCCG ACTACATCAA TGCCAGCTTC CTGGATGGCT ATCGACAGCA GAAGGCCTAC 
ATAGCCACAC AGGGGCCTCT GGCGGAGAGC ACCGAGGATT TCTGGCGCAT GTTATGGGAG 
CACAACTCCA CCATCATCGT CATGCTGACC AGGCTTCGGG AGATGGGCCG GGAGAAATGC 
CACCAGTACT GGCCTGCAGA GCGCTCTGCT CGCTACCAGT ACTTTGTCGT CGACCCGATG 
GCTGAGTACA ACATGCCCCA GTATATTCTC CGTGAGTTCA AGGTCACGGA TGCCCGGGAT 
GGGCAGTCGA GGACAATCCG GCAGTTCCAG TTCACAGACT GGCCGGAGCA GGGCGTGCCT 
AAAACGGGCG AGGGCTTCAT CGACTTCATC GGGCAGGTGC ACAAGACCAA GGAGCAGTTC 
GGGCAGGACG GGCCCATCAC GGTGCACTGC AGTGCTGGTG TGGGCCGCAC TGGGGTGTTC 
ATCACGCTGA GCATTGTCCT GGAGCGGATG CGCTACGAAG GCGTGGTTGA CATGTTCCAG 
ACCGTGAAGA CCCTGCGCAC ACAGCGCCCA GCCATGGTGC AGACGGAGGA CCAGTATCAG 
CTGTGCTACC GCGCGGCCCT GGAGTACCTC GGAAGCTTTG ACCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094549.1
CDS	279..5975
Translation	MTPEPAPGRTMVPLVPALVMLGLVAGAHGDSKPVFVKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPLGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPAASNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLVVTETTATSVTLTWDSGNSEPVSYYGIQYRPAGAEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDSRRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEVDSDTRIQLSWLLPPQERIVKYELVYWAAEDEGQQHKVTFDPTSSYTVEDLKPDTLYRFQLAARSELGVGVFTPTIEARTAQSTPSAPPQKVTCVSVGSTTVRVSWVPPPADSRNGVITQYSVAYEAVDGEDRGRHVVDGIGREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGAPIIQDVMLAEAQETTISGLTPETTYSITVAAYTTKGDGARSKPKIVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYRRADEARPSTIDFGKDDQHFTVTGLHKGATYIFRLTAKNRAGLGEEFEKEITTPEDVPSGFPQNLRVIGLTTSTTELIWDPPVLAERNGRITNYTVVYRDINSQQELQNVTADTHLTLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPVEQVFAKNFRVEAAMKTSVLLSWEVPDSYKSAVPFRILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPLPASAYIEDGRFTLTMPRVQEPALVRWFYIMVVPIDRMGGSMLAPQWSTPEELELDELLEAIEQGGGERLRRRRQTERLKPYVAAQVDVLPETFTLGDKKNYQGFYNRPLSPDLSYQCFVLASLKEPVDQKRYACSPYSDEIVVQVTPAQQQEEPELLWVTGPVLAVILIVLIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSIDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETYGLIQVTLLDTVELATYTVRTFALYKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRAQRNYMVQTEDQYVFIHEALLEAAMCGHTEVPARNLYAHIQKLGQVPPGESVTAMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHNSTIIVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	NM_001101079.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein tyrosine phosphatase receptor type F (PTPRF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101079.1

ORF Insert Sequence:

ATGACCCCTG AGCCAGCCCC AGGGAGGACG ATGGTGCCCC TTGTCCCTGC ACTTGTGATG 
CTTGGTTTGG TGGCAGGTGC CCATGGCGAC AGCAAACCTG TCTTTGTCAA GGTCCCCGAG 
GACCAGACTG GATTGTCAGG AGGGGTGGCC TCCTTCGTGT GCCAAGCCAC GGGGGAACCC 
AAGCCGCGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTCGAGGTC 
ATCGAGTTTG ATGACGGGGC AGGGTCTGTG CTGCGGATCC AGCCACTGCG GGTGCAGCGA 
GACGAAGCCA TCTATGAGTG CACGGCCACC AACAGTCTGG GGGAGATCAA CACCAGTGCC 
AAGCTCTCAG TGCTCGAAGA GGAACAGCTG CCCCTTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCCCGC ACGGCCACTA TGCTGTGTGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC CGCCGCGAGC 
AACGGCCGCA TCAAGCAACT GCGTTCAGGT GCCTTGCAGA TTGAGAGCAG CGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCTGCAG GCACTCGTTA CTCGGCCCCT 
GCCAACCTAT ATGTGCGAGT GCGCCGCGTG GCTCCGCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGCGG CAGCGTGAAC CTGACGTGCG TCGCCGTTGG CGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAA GAGCTCACCA AGGAGGACGA GATGCCGGTC 
GGCCGCAATG TGCTGGAGCT CAGCAACGTG GTGCGCTCCG CCAACTACAC TTGCGTGGCC 
ATCTCCTCCC TGGGCATGAT CGAGGCCACG GCCCAGGTCA CCGTGAAAGC CCTGCCGAAG 
CCTCCAATTG ACCTTGTGGT GACAGAGACA ACTGCCACCA GCGTCACTCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT ATCCTACTAT GGCATCCAGT ACCGCCCAGC GGGCGCGGAG 
GGCCCCTTCC AGGAGGTGGA CGGCGTGGCC ACCACCCGCT ACAGCATCGG CGGCCTGAGC 
CCTTTCTCAG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGGCG CGGGCCGCCC 
AGCGAGGCGG TGCGGGCGCG CACGGGGGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTAAGCGC CAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCT 
AACGGCCTGG TGCGGGGCTA CCGCGTCTAC TACACCCCCG ACTCCCGCCG CCCTCTGAGC 
GCCTGGCACA AGCACAACAC AGACGCCGGG CTCCTCACCA CTGTGGGCAG CCTGCTGCCT 
GGCATCACCT ACAGCCTGCG TGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCCCCCAGC 
CCCACCATCC AGGTCAAGAC GCAGCAGGGA GTGCCTGCCC AGCCCGCGGA CTTCCAGGCC 
GAGGTGGACT CAGACACCAG GATCCAGCTC TCGTGGCTGC TGCCCCCGCA GGAGCGGATC 
GTCAAGTATG AGCTGGTGTA CTGGGCGGCA GAGGACGAGG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CGTCCTCCTA TACCGTAGAG GACCTGAAGC CCGACACACT GTACCGCTTC 
CAGCTGGCCG CCCGCTCTGA GCTGGGGGTG GGAGTCTTCA CCCCCACCAT TGAGGCTCGC 
ACAGCACAGT CCACCCCCTC CGCACCCCCC CAGAAGGTGA CGTGTGTGAG CGTGGGCTCC 
ACCACCGTCC GGGTAAGTTG GGTCCCGCCG CCGGCCGACA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCGGTGGAC GGCGAGGACC GTGGGCGGCA CGTGGTGGAC 
GGCATCGGCC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACGGACGTG GGCCCCGGCC CCGAGAGCAG CCCGGTGCTG 
GTCCGCACCG ACGAGGACGT GCCCAGTGGG CCACCGCGGA AGGTGGAGGT GGAGCCGCTC 
AACTCCACTG CCGTGCGCGT CTCCTGGAAG CTGCCCGTCC CCAGCAAGCA GCATGGCCAG 
ATCCGCGGCT ACCAGGTCAC CTACGTGCGG CTGGAGAATG GCGAGCCCCG CGGCGCCCCC 
ATCATCCAGG ACGTCATGCT GGCCGAGGCC CAGGAAACCA CCATCAGCGG CCTGACCCCG 
GAGACCACCT ACTCCATCAC CGTGGCTGCC TACACCACCA AGGGGGACGG TGCCCGCAGC 
AAGCCCAAAA TTGTCACGAC CACAGGGGCA GTCCCAGGAC GGCCCACCAT GATGGTCAGC 
ACCACGGCCA TGAACACGGC GCTGCTCCAG TGGCACCCGC CCAAGGAGCT GCCCGGGGAG 
CTCCTGGGCT ACCGGCTGCA GTACCGCCGA GCCGACGAGG CGCGGCCCAG CACCATCGAT 
TTTGGCAAGG ACGACCAGCA CTTTACGGTC ACTGGCCTGC ACAAAGGGGC CACCTACATC 
TTCCGGCTCA CTGCCAAGAA CCGGGCCGGC CTGGGCGAGG AGTTCGAGAA GGAAATCACG 
ACCCCCGAGG ACGTGCCCAG CGGTTTCCCC CAAAACCTGC GCGTGATAGG GCTGACCACA 
TCTACCACGG AACTGATCTG GGACCCCCCA GTGCTGGCGG AGAGGAACGG GCGCATCACC 
AACTACACCG TGGTGTACCG TGACATCAAC AGCCAGCAGG AGCTTCAGAA CGTCACTGCC 
GACACCCACC TGACGCTCTC CGGCCTCAAG CCAGACACCA CTTACGACAT CAAGGTCCGC 
GCACGCACCA GCAAGGGCGC CGGCCCGCTC AGCCCCAGCA TCCAGTCCCG GACCATGCCC 
GTGGAGCAAG TGTTTGCCAA GAACTTCCGT GTGGAGGCTG CAATGAAGAC ATCTGTGCTG 
CTCAGCTGGG AGGTGCCCGA CTCCTACAAG TCAGCAGTGC CCTTCAGGAT CCTGTACAAT 
GGGCAGAGCG TGGAGGTGGA CGGGCACTCC ATGCGGAAGC TGATTGCAGA CCTGCAGCCC 
AACACTGAAT ACTCATTTGT GCTGATGAAC CGCGGCAGCA GCGCGGGGGG CCTGCAACAC 
CTCGTGTCCA TCCGCACGGC CCCCGACCTC CTGCCGCACA AGCCACTGCC AGCCTCGGCC 
TACATCGAGG ACGGCCGCTT CACCCTCACC ATGCCCCGCG TGCAGGAACC CGCACTGGTC 
AGGTGGTTCT ACATCATGGT GGTGCCCATT GACCGCATGG GAGGGAGCAT GCTGGCGCCA 
CAGTGGAGCA CGCCCGAGGA GCTGGAGCTG GACGAGCTGC TGGAGGCCAT CGAGCAGGGC 
GGCGGGGAGC GGCTGCGGCG CCGGCGGCAG ACGGAGCGGC TGAAGCCATA CGTGGCCGCT 
CAGGTGGACG TTCTCCCGGA GACCTTCACC TTGGGGGACA AGAAGAACTA TCAAGGCTTC 
TACAACCGGC CGCTGTCTCC AGACCTGAGC TACCAGTGCT TTGTGCTCGC CTCCCTGAAG 
GAACCCGTGG ACCAGAAGCG CTATGCCTGC AGCCCCTACT CCGATGAGAT CGTGGTCCAG 
GTGACACCAG CCCAGCAGCA GGAGGAACCC GAGCTGCTGT GGGTGACGGG CCCCGTCCTG 
GCAGTCATTC TCATCGTCCT CATTGTCATC GCCATTCTCC TGTTCAAGAG GAAAAGGACC 
CACTCCCCAT CGTCCAAGGA TGAGCAGTCA ATTGGGCTGA AGGACTCCTT GCTGGCCCAC 
TCCTCCGACC CTGTGGAGAT GCGGCGGCTC AACTACCAGA CCCCAGGTAT GCGAGACCAC 
CCGCCCATCC CCATCACCGA CCTAGCTGAC AACATCGAGC GCCTCAAAGC CAATGACGGC 
CTCAAGTTCT CCCAGGAGTA CGAGTCAATT GACCCAGGAC AGCAGTTCAC GTGGGAGAAT 
TCAAACCTGG AGGTGAACAA GCCGAAGAAC CGCTATGCCA ACGTCATTGC TTACGACCAC 
TCTCGAGTCA TCCTTACCTC CATCGATGGT GTCCCCGGGA GCGACTACAT CAATGCCAAT 
TACATCGACG GCTACCGCAA GCAGAACGCC TACATTGCCA CGCAGGGCCC GCTGCCTGAG 
ACCATGGGCG ATTTCTGGCG GATGGTGTGG GAACAGCGCA CAGCCACTGT GGTCATGATG 
ACGCGGCTGG AGGAGAAGTC CCGGGTGAAA TGTGACCAGT ACTGGCCAGC TCGTGGCACC 
GAGACCTATG GCCTCATCCA GGTGACCCTG CTGGACACGG TGGAGCTGGC CACCTACACT 
GTGCGCACCT TCGCGCTCTA CAAGAGTGGC TCCAGTGAGA AGCGGGAGCT GCGTCAGTTC 
CAGTTCATGG CCTGGCCAGA CCACGGAGTC CCCGAGTACC CGACCCCTAT CCTGGCCTTC 
CTGCGGCGGG TCAAGGCTTG CAACCCCCTG GACGCGGGGC CCATGGTGGT GCACTGCAGC 
GCGGGCGTGG GCCGCACGGG CTGCTTCATC GTCATCGACG CCATGCTGGA GCGGATGAAG 
CACGAGAAGA CAGTGGACAT CTACGGCCAC GTGACGTGCA TGCGGGCGCA GCGCAACTAC 
ATGGTGCAGA CTGAGGACCA GTACGTGTTC ATCCACGAGG CGCTGCTGGA GGCCGCCATG 
TGTGGACACA CGGAGGTGCC CGCCCGCAAC CTCTACGCCC ACATCCAGAA GCTGGGCCAA 
GTGCCTCCCG GGGAGAGTGT GACTGCCATG GAGCTGGAGT TCAAGTTGCT GGCCAACTCC 
AAGGCCCACA CATCCCGCTT CATCAGCGCC AACCTGCCCT GCAACAAGTT CAAGAACCGC 
CTGGTGAACA TCATGCCCTA CGAACTGACC CGTGTGTGTC TGCAGCCCAT CCGCGGCGTG 
GAGGGCTCCG ACTACATCAA TGCCAGCTTC CTGGATGGCT ATCGACAGCA GAAGGCCTAC 
ATAGCCACAC AGGGGCCTCT GGCGGAGAGC ACCGAGGATT TCTGGCGCAT GTTATGGGAG 
CACAACTCCA CCATCATCGT CATGCTGACC AGGCTTCGGG AGATGGGCCG GGAGAAATGC 
CACCAGTACT GGCCTGCAGA GCGCTCTGCT CGCTACCAGT ACTTTGTCGT CGACCCGATG 
GCTGAGTACA ACATGCCCCA GTATATTCTC CGTGAGTTCA AGGTCACGGA TGCCCGGGAT 
GGGCAGTCGA GGACAATCCG GCAGTTCCAG TTCACAGACT GGCCGGAGCA GGGCGTGCCT 
AAAACGGGCG AGGGCTTCAT CGACTTCATC GGGCAGGTGC ACAAGACCAA GGAGCAGTTC 
GGGCAGGACG GGCCCATCAC GGTGCACTGC AGTGCTGGTG TGGGCCGCAC TGGGGTGTTC 
ATCACGCTGA GCATTGTCCT GGAGCGGATG CGCTACGAAG GCGTGGTTGA CATGTTCCAG 
ACCGTGAAGA CCCTGCGCAC ACAGCGCCCA GCCATGGTGC AGACGGAGGA CCAGTATCAG 
CTGTGCTACC GCGCGGCCCT GGAGTACCTC GGAAGCTTTG ACCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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PTPRF cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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