ptprfa gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021476762.1	XP_021332437.1	receptor-type tyrosine-protein phosphatase F isoform X2
XM_021476763.1	XP_021332438.1	receptor-type tyrosine-protein phosphatase F isoform X3
XM_021476767.1	XP_021332442.1	receptor-type tyrosine-protein phosphatase F isoform X7
XM_021476771.1	XP_021332446.1	receptor-type tyrosine-protein phosphatase F isoform X11
XM_021476772.1	XP_021332447.1	receptor-type tyrosine-protein phosphatase F isoform X12
NM_001311348.1	NP_001298277.1	receptor-type tyrosine-protein phosphatase F precursor
XM_021476764.1	XP_021332439.1	receptor-type tyrosine-protein phosphatase F isoform X4
XM_021476766.1	XP_021332441.1	receptor-type tyrosine-protein phosphatase F isoform X6
XM_021476773.1	XP_021332448.1	receptor-type tyrosine-protein phosphatase F isoform X13
XM_021476760.1	XP_021332435.1	receptor-type tyrosine-protein phosphatase F isoform X1
XM_021476768.1	XP_021332443.1	receptor-type tyrosine-protein phosphatase F isoform X8
XM_021476765.1	XP_021332440.1	receptor-type tyrosine-protein phosphatase F isoform X5
XM_021476769.1	XP_021332444.1	receptor-type tyrosine-protein phosphatase F isoform X9
XM_021476761.1	XP_021332436.1	receptor-type tyrosine-protein phosphatase F isoform X1
XM_021476770.1	XP_021332445.1	receptor-type tyrosine-protein phosphatase F isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa142902	XM_021476760.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142902	XM_021476761.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142903	XM_021476762.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142904	XM_021476763.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142905	XM_021476764.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142906	XM_021476765.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142907	XM_021476766.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142908	XM_021476767.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142909	XM_021476768.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142910	XM_021476769.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142911	XM_021476770.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142912	XM_021476771.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142913	XM_021476772.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142914	XM_021476773.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa39492	NM_001311348.1 Latest version!	Danio rerio protein tyrosine phosphatase receptor type Fa (ptprfa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ptprfa ( XM_021476760.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332435.1 Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa142902
Clone ID Related Accession (Same CDS sequence)	XM_021476760.1 , XM_021476761.1
Accession Version	XM_021476760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGACC AGCGGGAAGT CCGACGGGTT 
CCCCCACGTT TTTCAATCCC TCCAACCAAT CACGAGGTGA TGCCGGGTGG CAGTGTGAAC 
CTCACCTGCG TGGCAGTGGG CGCTCCAATG CCCTATGTGA AGTGGATGAA GGGCGAACAG 
GAGCTGACCA AAGAAGAGGA GATGCCCATC GGCCGAAATG TGCTGGAGCT CACCAACATC 
CGCCAGTCGG GGAACTACAC CTGCGTGGCC ATATCATCAC TGGGCATGAT CGACACCACA 
GCGCAGATCA CTGTTAAAGC TCTTCCTCGT CCTCCAGCCT CTCTGATTGT CACTGAAACC 
ACCGCCACCA GTGTGACTTT AACATGGGAT TCTGGCAACC CAGAACCCGT ATCATATTAC 
GTGATCCAGT ATCGATCCAA AATCTCTGAC AATGGTTTCC AGGAGGTGGA CGGCGTGGCA 
TCCACCCGCT ACAGCATCGG CGGGTTGAGT CCGTACTCAG AGTATGAGTT TCGAGTAATG 
GCGGTCAATA ACATTGGTCG CGGGCCGCCC AGCGGGATGG TGGAGACTCG TACCAGTGAA 
CAGGCACCGT CATCTCCTCC GCTGCGCGTT CAAGCCCGGA TGTTGAGCGC TACGACTATG 
CTGGTGCAAT GGGAACCTCC TGAAGAGCCC AACGGACAGA TTCGAGGATA TCGAGTGTAC 
TACACGTCTG ACCTGCACTT CCCGCTCAGC ACATGGCAGA AGCACAACAC TGAGGACAGC 
AGTCTGACCA CCATCTCCGG CCTGGTTCCT GACATCACGT ATGGGCTCCG TGTGCTGGCC 
TTCACCTCTG TTGGAGATGG ACCACCTTCC GATATACTGC AGGTTAAAAC ACAGCAAGGA 
GTCCCTGCTC AGCCGACTGG TTTTGAAGCT GAAGCCGAGT TGGACACGCG GATCATGCTG 
TCATGGTTGT GGCCCGTCCA AGATCAGATC ACCAAATATG AGCTCACATA CTGGGAGGCT 
GACTCTGACA ACAAGCATCA TGTCATCTTT GACCCGGCCG GTTCATATGC TATTGACAGC 
CTAAAGCCAG ACACTCTCTA TAAATTCTCT CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG 
GTTTACACAC AGCCTATCGA GGCCCGCACA GCCCAGTCCG CCCCGTCCGC TCCCCCACAA 
GAGGTGCACC TGATCAGCCT GAGCTCCAGC AGCATCAAGG TGAGTTGGGT GGCCCCTCCG 
GCCGCCAGCC GGCACGGCAG CATCGTCCGC TACTCTCTGG CCTATCAGGC AGTGTCTGGC 
GAGGACCAGG AGCGCCACGA GGTCAAAGAC ATCCCTGCTG ATGTGTCCAG CTATGTTCTG 
GAGGGTCTGG AGAAATGGAC TGAGTATACG GTGTGGGTCA AAGCGCACAC AGATGTGGGT 
CCCGGGCCAG AAAGTGGCAG CACCCGCATC AGAACCCAAG AGGATGTGCC TGGTGCTCCT 
CCACGCAGGG TTGAGGTGGA CAACATCAAC TCCACTGCAC TGCGTGTGTC CTGGAAGCCG 
CCTCTGCAGC AGAAACAACA CGGCCACATA CGTGGATATC AGGTGGTTTA CTCACGCCTG 
GAGAATGGAG AGCCGCGCGG ACAGCCCATC ATTCTGGACG TGGCGCTGCC AGAGGCACAG 
GAGGCCGTGA TCAGTGGTCT TCTCCCTGAA ACCACATATT CAGTGACTGT AGCCGCGTAC 
ACCACTAAAG GAGATGGAGC GCGGAGCAAA GCCAAAGTGG TGACCACAAC AGGAGCAGTT 
CCAGGAAAGC CCACTATGAT CATCAGCACC ACCATAGGCA ACACGGCTCT GATCCAGTGG 
CAGCCGCCTA AAGAGATGGT GGGAGAGCTG ATGGGCTACA GACTCCGCTA TAAGCGTGAA 
GAAGAGGATA TTTACACTGT CCGAGACTTC AGACGCACTG ATGACCACTT CACTGTCACC 
GGCCTGCACA AGGGCGCGAC GTACATCTTC AAACTCTGCG CTAAAAACCG CGCCGGGAAT 
GGAGAGGAGT ACAGCAAAGA GATCAGCACC CCGGAGGATG TGCCCAGCAG TTACCCACAA 
AACCTCAAAG TCGTTGGATT GACCACAACC ACCACAAAAC TAGCCTGGGA TCCTCCGCCG 
CTGCCCGAGA GGAATGGGAA GATCGTACGA TATGTGGTGG TTTATCGAGA CATTAACAGC 
CATCAGAATC AGACCAACGG CACAGCGGAC ACGGAGATGA CCATTCAGGG CCTGAAGCCG 
GACACCACCT ATGATATCAG GGTGAGGGCG TTTACAGGGA AAGGTGGAGG TCCCATCAGC 
CCCAGCATCC AGAGCAGAAC CATGTCTACC TCCATGCCTG AGTTCACGAA AAACTTTGGT 
GTGAAAGCCG TGATGAAGAC CTCTGTCCTG CTCACATGGG AGGTGCCAGA AACATACAAG 
TCTCAAGTGC CTTTCAAGAT CCTGTATAAC CAGCAGAGTG TGGAGGTCCA GGGTGACCTG 
AAGAGGAAGC TGATTACGCG TCTGCAGCCG GACACTGATT ACTCCTTTGT CCTGATGAGT 
CGGGGGAACA GCGCTGGCGG ACTGCAGCAG CAGGTCTCCA TCCGCACCGC TCCAGACCTG 
TTCACTACCA AACCAGCACA CCAGAAACTA GACCCAGAGG AGGGCGGCAA ACTCGCCATC 
ACACTGCCCG AAGTGCACAA CAGCGCCCTC GTCAGGTGGT TTTATATCGT GGTTGTACCA 
GTAACTCCTT CAACTCTCCG CCATTGGGAA AACCCAGAAG ATATTGATCT GGATGAGCTG 
ATGGGGAATA ATAGAGACGC AGCTGTGAGG AGGAGGAGAA GACAAACTCA GGACTTTCTG 
CGGCCTTACA TTGCTGCCAA GCTGGAGTCT CTTCCAGAAA GATTTGTGTT GGGAGATGAG 
AAAACCTACA GCAACTACTA CAACAAACCT CTGCCGGGAC AGCAGCAGTA TCGCACATTT 
ATACTGGCGG CACTGAAAGA CAGAGACTCG ACGTTCACAG TGAGTCCGTT CTCTGAGCCC 
ATGATGGTGA AGCTCCACAG CGGGATGTCC CGTCACGTGG AGGACCCGGA GATGCTGTGG 
GTGATGGGAC CAGTGTTAGC CGTGGTCCTC ATCATCATCA TCGTCATCGC CATCCTCCTC 
TTCAAGAGCA AACAGGAGAG GAAGCGCACG TCTCCCTCAG CTAAAGATGA GCACTCAGCT 
GGAGTTAAAG ATCACCTGCT GGCTCATTCG TCAGATCCAG TGGAGATGAG GAGGCTCAAC 
TACCAGACAC AAGGTTCCAG TGCCTTCAAC TGTCCCAGCA CTTCAAGAAT GCGGGAACAC 
CCTCCAGTGT CTGTGTGTGC TTTGGCTGAC CACATCGAGC GGCTGAGAGC CAACGACAGC 
CTCCGCTTCT CGCAGGAGTA TGAGTCTGTT GACCCTGGCC AGCAGTTCAC CTGGGAAAAC 
TCCAACTTAG AGGTCAACAA GCCCAAGAAC CGCTACGCAA ACGTCATCGC TTACGATCAC 
TCCAGGGTTG TGCTGACTCC TGTGGATGGT GTTCCCGGAA GTGACTACAT CAATGGAAAC 
TACATTGATG GCTACCGTAA GCAGAACGCC TACATCGCCA CGCAGGGTCC GCTGCCTGAG 
ACGCTCAGTG ACTTCTGGAG GATGGTGTGG GAACAGAGAA CCAGCACTAT CGTCATGATG 
ACCCGACTGG AGGAGAAGAG CAGGGTGAAG TGTGATCAGT ACTGGCCAAG CCGAGGAACA 
GAGACTTACG GGATGATTCA AGTGTCCATG CTGGATACGG TGGAGCTGGC CACATACAGC 
GTCAGAACCT TCGCTCTCTA CAAGAATGGA TCCAGCGAGA AACGGGAGGT TCGGCAGTTC 
CAGTTCATGG CGTGGCCGGA TCACGGTGTT CCCGAATATC CCACACCCAT TTTAGCCTTC 
TTACGCAGGG TCAAAGCCTG CAACCCACCT GACGCGGGAC CCATGGTGGT GCACTGCAGC 
GCTGGCGTGG GTCGGACGGG CTGTTTCATC GTAATAGACG CCATGCTGGA GCGGATGAAG 
CACGAGAAGT CGGTGGACAT CTACGGTCAC GTGACCTGCA TGCGTTCTCA GAGGAACTAC 
ATGGTGCAGA CAGAGGATCA GTACATTTTC ATTCACGAGG CCCTGCTGGA GGCTGCCACC 
TGCGGGAACA CAGAGGTGCC GGCGCGGAGC CTCTACACAC ACATCCAGAA ACTCACACAG 
GCGCCGCCTG GAGACACCGT CACCGCCATG GAGCTCGAAT TTAAGAAACT GGCCAACTCT 
AAAGCACACA CCTCCAGATT CATCAGCGCT AGTCTGCCCT GCAACAAATT CAAGAACCGG 
CTGGTGAACA TCATGCCTTT CGAGTCCAGC CGTGTTTGTC TGCAGCCAAT CAGAGGCGTG 
GAGGGCTCCG ACTACATCAA CGCCAGCTTC ATCGACGGAT ACAGGCAGCA GAAGGCATAT 
ATCGCCACTC AAGGCCCGCT GTCTGAAACC ACAGAGGACT TCTGGAGGAT GCTTTGGGAG 
CACAACTCCA CCATCGTGGT GATGCTGACG AAGCTTAGAG AGATGGGACG GGAGAAATGT 
CATCAGTACT GGCCTGCAGA GAGATCTGCG CGCTACCAGT ATTTCGTTGT GGATCCTATG 
GCTGAATATA ACATGCCACA GTACATCTTA CGGGAGTTTA AAGTCACAGA CGCCAGAGAT 
GGCCAGTCTC GGACTATCCG ACAGTTTCAG TTCACAGATT GGCCGGAACA AGGTGTGCCA 
AAGACAGGAG AGGGCTTTAT TGATTTTATT GGTCAAGTCC ACAAAACCAA GGAGCAGTTT 
GGACAGGACG GACCAATTAC TGTTCACTGC AGTGCCGGTG TGGGCAGAAC AGGCGTCTTC 
ATCACTTTAA GTATTGTTCT GGAGCGGATG AGGTATGAAG GAGTGGTGGA CATGTTCCAG 
ACCATAAAAA CACTCCGCAC GCAGAGACCT GCCATGGTAC AGACCGAGGA CCAGTACCAG 
CTGTGCTACA GAGCAGCTCT GGAGTACCTG GGCAGCTTCG ATCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332435.1
CDS	1459..7215
Translation	MVPQTRQGSARHGATSLLLLLCLAVPIHADSMPSFVKTPEDQTGISGGVASFVCQAVGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEEDQIPHGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEISWFKDFLPVDINSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAVNSAGTRYSAPANLYVRDQREVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMKGEQELTKEEEMPIGRNVLELTNIRQSGNYTCVAISSLGMIDTTAQITVKALPRPPASLIVTETTATSVTLTWDSGNPEPVSYYVIQYRSKISDNGFQEVDGVASTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSGMVETRTSEQAPSSPPLRVQARMLSATTMLVQWEPPEEPNGQIRGYRVYYTSDLHFPLSTWQKHNTEDSSLTTISGLVPDITYGLRVLAFTSVGDGPPSDILQVKTQQGVPAQPTGFEAEAELDTRIMLSWLWPVQDQITKYELTYWEADSDNKHHVIFDPAGSYAIDSLKPDTLYKFSLAARSEMGLGVYTQPIEARTAQSAPSAPPQEVHLISLSSSSIKVSWVAPPAASRHGSIVRYSLAYQAVSGEDQERHEVKDIPADVSSYVLEGLEKWTEYTVWVKAHTDVGPGPESGSTRIRTQEDVPGAPPRRVEVDNINSTALRVSWKPPLQQKQHGHIRGYQVVYSRLENGEPRGQPIILDVALPEAQEAVISGLLPETTYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTIGNTALIQWQPPKEMVGELMGYRLRYKREEEDIYTVRDFRRTDDHFTVTGLHKGATYIFKLCAKNRAGNGEEYSKEISTPEDVPSSYPQNLKVVGLTTTTTKLAWDPPPLPERNGKIVRYVVVYRDINSHQNQTNGTADTEMTIQGLKPDTTYDIRVRAFTGKGGGPISPSIQSRTMSTSMPEFTKNFGVKAVMKTSVLLTWEVPETYKSQVPFKILYNQQSVEVQGDLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFTTKPAHQKLDPEEGGKLAITLPEVHNSALVRWFYIVVVPVTPSTLRHWENPEDIDLDELMGNNRDAAVRRRRRQTQDFLRPYIAAKLESLPERFVLGDEKTYSNYYNKPLPGQQQYRTFILAALKDRDSTFTVSPFSEPMMVKLHSGMSRHVEDPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRTSPSAKDEHSAGVKDHLLAHSSDPVEMRRLNYQTQGSSAFNCPSTSRMREHPPVSVCALADHIERLRANDSLRFSQEYESVDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINGNYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRTSTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVSMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARSLYTHIQKLTQAPPGDTVTAMELEFKKLANSKAHTSRFISASLPCNKFKNRLVNIMPFESSRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLSETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTIKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021476760.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476760.1

ORF Insert Sequence:

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGACC AGCGGGAAGT CCGACGGGTT 
CCCCCACGTT TTTCAATCCC TCCAACCAAT CACGAGGTGA TGCCGGGTGG CAGTGTGAAC 
CTCACCTGCG TGGCAGTGGG CGCTCCAATG CCCTATGTGA AGTGGATGAA GGGCGAACAG 
GAGCTGACCA AAGAAGAGGA GATGCCCATC GGCCGAAATG TGCTGGAGCT CACCAACATC 
CGCCAGTCGG GGAACTACAC CTGCGTGGCC ATATCATCAC TGGGCATGAT CGACACCACA 
GCGCAGATCA CTGTTAAAGC TCTTCCTCGT CCTCCAGCCT CTCTGATTGT CACTGAAACC 
ACCGCCACCA GTGTGACTTT AACATGGGAT TCTGGCAACC CAGAACCCGT ATCATATTAC 
GTGATCCAGT ATCGATCCAA AATCTCTGAC AATGGTTTCC AGGAGGTGGA CGGCGTGGCA 
TCCACCCGCT ACAGCATCGG CGGGTTGAGT CCGTACTCAG AGTATGAGTT TCGAGTAATG 
GCGGTCAATA ACATTGGTCG CGGGCCGCCC AGCGGGATGG TGGAGACTCG TACCAGTGAA 
CAGGCACCGT CATCTCCTCC GCTGCGCGTT CAAGCCCGGA TGTTGAGCGC TACGACTATG 
CTGGTGCAAT GGGAACCTCC TGAAGAGCCC AACGGACAGA TTCGAGGATA TCGAGTGTAC 
TACACGTCTG ACCTGCACTT CCCGCTCAGC ACATGGCAGA AGCACAACAC TGAGGACAGC 
AGTCTGACCA CCATCTCCGG CCTGGTTCCT GACATCACGT ATGGGCTCCG TGTGCTGGCC 
TTCACCTCTG TTGGAGATGG ACCACCTTCC GATATACTGC AGGTTAAAAC ACAGCAAGGA 
GTCCCTGCTC AGCCGACTGG TTTTGAAGCT GAAGCCGAGT TGGACACGCG GATCATGCTG 
TCATGGTTGT GGCCCGTCCA AGATCAGATC ACCAAATATG AGCTCACATA CTGGGAGGCT 
GACTCTGACA ACAAGCATCA TGTCATCTTT GACCCGGCCG GTTCATATGC TATTGACAGC 
CTAAAGCCAG ACACTCTCTA TAAATTCTCT CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG 
GTTTACACAC AGCCTATCGA GGCCCGCACA GCCCAGTCCG CCCCGTCCGC TCCCCCACAA 
GAGGTGCACC TGATCAGCCT GAGCTCCAGC AGCATCAAGG TGAGTTGGGT GGCCCCTCCG 
GCCGCCAGCC GGCACGGCAG CATCGTCCGC TACTCTCTGG CCTATCAGGC AGTGTCTGGC 
GAGGACCAGG AGCGCCACGA GGTCAAAGAC ATCCCTGCTG ATGTGTCCAG CTATGTTCTG 
GAGGGTCTGG AGAAATGGAC TGAGTATACG GTGTGGGTCA AAGCGCACAC AGATGTGGGT 
CCCGGGCCAG AAAGTGGCAG CACCCGCATC AGAACCCAAG AGGATGTGCC TGGTGCTCCT 
CCACGCAGGG TTGAGGTGGA CAACATCAAC TCCACTGCAC TGCGTGTGTC CTGGAAGCCG 
CCTCTGCAGC AGAAACAACA CGGCCACATA CGTGGATATC AGGTGGTTTA CTCACGCCTG 
GAGAATGGAG AGCCGCGCGG ACAGCCCATC ATTCTGGACG TGGCGCTGCC AGAGGCACAG 
GAGGCCGTGA TCAGTGGTCT TCTCCCTGAA ACCACATATT CAGTGACTGT AGCCGCGTAC 
ACCACTAAAG GAGATGGAGC GCGGAGCAAA GCCAAAGTGG TGACCACAAC AGGAGCAGTT 
CCAGGAAAGC CCACTATGAT CATCAGCACC ACCATAGGCA ACACGGCTCT GATCCAGTGG 
CAGCCGCCTA AAGAGATGGT GGGAGAGCTG ATGGGCTACA GACTCCGCTA TAAGCGTGAA 
GAAGAGGATA TTTACACTGT CCGAGACTTC AGACGCACTG ATGACCACTT CACTGTCACC 
GGCCTGCACA AGGGCGCGAC GTACATCTTC AAACTCTGCG CTAAAAACCG CGCCGGGAAT 
GGAGAGGAGT ACAGCAAAGA GATCAGCACC CCGGAGGATG TGCCCAGCAG TTACCCACAA 
AACCTCAAAG TCGTTGGATT GACCACAACC ACCACAAAAC TAGCCTGGGA TCCTCCGCCG 
CTGCCCGAGA GGAATGGGAA GATCGTACGA TATGTGGTGG TTTATCGAGA CATTAACAGC 
CATCAGAATC AGACCAACGG CACAGCGGAC ACGGAGATGA CCATTCAGGG CCTGAAGCCG 
GACACCACCT ATGATATCAG GGTGAGGGCG TTTACAGGGA AAGGTGGAGG TCCCATCAGC 
CCCAGCATCC AGAGCAGAAC CATGTCTACC TCCATGCCTG AGTTCACGAA AAACTTTGGT 
GTGAAAGCCG TGATGAAGAC CTCTGTCCTG CTCACATGGG AGGTGCCAGA AACATACAAG 
TCTCAAGTGC CTTTCAAGAT CCTGTATAAC CAGCAGAGTG TGGAGGTCCA GGGTGACCTG 
AAGAGGAAGC TGATTACGCG TCTGCAGCCG GACACTGATT ACTCCTTTGT CCTGATGAGT 
CGGGGGAACA GCGCTGGCGG ACTGCAGCAG CAGGTCTCCA TCCGCACCGC TCCAGACCTG 
TTCACTACCA AACCAGCACA CCAGAAACTA GACCCAGAGG AGGGCGGCAA ACTCGCCATC 
ACACTGCCCG AAGTGCACAA CAGCGCCCTC GTCAGGTGGT TTTATATCGT GGTTGTACCA 
GTAACTCCTT CAACTCTCCG CCATTGGGAA AACCCAGAAG ATATTGATCT GGATGAGCTG 
ATGGGGAATA ATAGAGACGC AGCTGTGAGG AGGAGGAGAA GACAAACTCA GGACTTTCTG 
CGGCCTTACA TTGCTGCCAA GCTGGAGTCT CTTCCAGAAA GATTTGTGTT GGGAGATGAG 
AAAACCTACA GCAACTACTA CAACAAACCT CTGCCGGGAC AGCAGCAGTA TCGCACATTT 
ATACTGGCGG CACTGAAAGA CAGAGACTCG ACGTTCACAG TGAGTCCGTT CTCTGAGCCC 
ATGATGGTGA AGCTCCACAG CGGGATGTCC CGTCACGTGG AGGACCCGGA GATGCTGTGG 
GTGATGGGAC CAGTGTTAGC CGTGGTCCTC ATCATCATCA TCGTCATCGC CATCCTCCTC 
TTCAAGAGCA AACAGGAGAG GAAGCGCACG TCTCCCTCAG CTAAAGATGA GCACTCAGCT 
GGAGTTAAAG ATCACCTGCT GGCTCATTCG TCAGATCCAG TGGAGATGAG GAGGCTCAAC 
TACCAGACAC AAGGTTCCAG TGCCTTCAAC TGTCCCAGCA CTTCAAGAAT GCGGGAACAC 
CCTCCAGTGT CTGTGTGTGC TTTGGCTGAC CACATCGAGC GGCTGAGAGC CAACGACAGC 
CTCCGCTTCT CGCAGGAGTA TGAGTCTGTT GACCCTGGCC AGCAGTTCAC CTGGGAAAAC 
TCCAACTTAG AGGTCAACAA GCCCAAGAAC CGCTACGCAA ACGTCATCGC TTACGATCAC 
TCCAGGGTTG TGCTGACTCC TGTGGATGGT GTTCCCGGAA GTGACTACAT CAATGGAAAC 
TACATTGATG GCTACCGTAA GCAGAACGCC TACATCGCCA CGCAGGGTCC GCTGCCTGAG 
ACGCTCAGTG ACTTCTGGAG GATGGTGTGG GAACAGAGAA CCAGCACTAT CGTCATGATG 
ACCCGACTGG AGGAGAAGAG CAGGGTGAAG TGTGATCAGT ACTGGCCAAG CCGAGGAACA 
GAGACTTACG GGATGATTCA AGTGTCCATG CTGGATACGG TGGAGCTGGC CACATACAGC 
GTCAGAACCT TCGCTCTCTA CAAGAATGGA TCCAGCGAGA AACGGGAGGT TCGGCAGTTC 
CAGTTCATGG CGTGGCCGGA TCACGGTGTT CCCGAATATC CCACACCCAT TTTAGCCTTC 
TTACGCAGGG TCAAAGCCTG CAACCCACCT GACGCGGGAC CCATGGTGGT GCACTGCAGC 
GCTGGCGTGG GTCGGACGGG CTGTTTCATC GTAATAGACG CCATGCTGGA GCGGATGAAG 
CACGAGAAGT CGGTGGACAT CTACGGTCAC GTGACCTGCA TGCGTTCTCA GAGGAACTAC 
ATGGTGCAGA CAGAGGATCA GTACATTTTC ATTCACGAGG CCCTGCTGGA GGCTGCCACC 
TGCGGGAACA CAGAGGTGCC GGCGCGGAGC CTCTACACAC ACATCCAGAA ACTCACACAG 
GCGCCGCCTG GAGACACCGT CACCGCCATG GAGCTCGAAT TTAAGAAACT GGCCAACTCT 
AAAGCACACA CCTCCAGATT CATCAGCGCT AGTCTGCCCT GCAACAAATT CAAGAACCGG 
CTGGTGAACA TCATGCCTTT CGAGTCCAGC CGTGTTTGTC TGCAGCCAAT CAGAGGCGTG 
GAGGGCTCCG ACTACATCAA CGCCAGCTTC ATCGACGGAT ACAGGCAGCA GAAGGCATAT 
ATCGCCACTC AAGGCCCGCT GTCTGAAACC ACAGAGGACT TCTGGAGGAT GCTTTGGGAG 
CACAACTCCA CCATCGTGGT GATGCTGACG AAGCTTAGAG AGATGGGACG GGAGAAATGT 
CATCAGTACT GGCCTGCAGA GAGATCTGCG CGCTACCAGT ATTTCGTTGT GGATCCTATG 
GCTGAATATA ACATGCCACA GTACATCTTA CGGGAGTTTA AAGTCACAGA CGCCAGAGAT 
GGCCAGTCTC GGACTATCCG ACAGTTTCAG TTCACAGATT GGCCGGAACA AGGTGTGCCA 
AAGACAGGAG AGGGCTTTAT TGATTTTATT GGTCAAGTCC ACAAAACCAA GGAGCAGTTT 
GGACAGGACG GACCAATTAC TGTTCACTGC AGTGCCGGTG TGGGCAGAAC AGGCGTCTTC 
ATCACTTTAA GTATTGTTCT GGAGCGGATG AGGTATGAAG GAGTGGTGGA CATGTTCCAG 
ACCATAAAAA CACTCCGCAC GCAGAGACCT GCCATGGTAC AGACCGAGGA CCAGTACCAG 
CTGTGCTACA GAGCAGCTCT GGAGTACCTG GGCAGCTTCG ATCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfa ( XM_021476761.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332436.1 Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X2, mRNA.

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CloneID	ODa142902
Clone ID Related Accession (Same CDS sequence)	XM_021476760.1 , XM_021476761.1
Accession Version	XM_021476761.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGACC AGCGGGAAGT CCGACGGGTT 
CCCCCACGTT TTTCAATCCC TCCAACCAAT CACGAGGTGA TGCCGGGTGG CAGTGTGAAC 
CTCACCTGCG TGGCAGTGGG CGCTCCAATG CCCTATGTGA AGTGGATGAA GGGCGAACAG 
GAGCTGACCA AAGAAGAGGA GATGCCCATC GGCCGAAATG TGCTGGAGCT CACCAACATC 
CGCCAGTCGG GGAACTACAC CTGCGTGGCC ATATCATCAC TGGGCATGAT CGACACCACA 
GCGCAGATCA CTGTTAAAGC TCTTCCTCGT CCTCCAGCCT CTCTGATTGT CACTGAAACC 
ACCGCCACCA GTGTGACTTT AACATGGGAT TCTGGCAACC CAGAACCCGT ATCATATTAC 
GTGATCCAGT ATCGATCCAA AATCTCTGAC AATGGTTTCC AGGAGGTGGA CGGCGTGGCA 
TCCACCCGCT ACAGCATCGG CGGGTTGAGT CCGTACTCAG AGTATGAGTT TCGAGTAATG 
GCGGTCAATA ACATTGGTCG CGGGCCGCCC AGCGGGATGG TGGAGACTCG TACCAGTGAA 
CAGGCACCGT CATCTCCTCC GCTGCGCGTT CAAGCCCGGA TGTTGAGCGC TACGACTATG 
CTGGTGCAAT GGGAACCTCC TGAAGAGCCC AACGGACAGA TTCGAGGATA TCGAGTGTAC 
TACACGTCTG ACCTGCACTT CCCGCTCAGC ACATGGCAGA AGCACAACAC TGAGGACAGC 
AGTCTGACCA CCATCTCCGG CCTGGTTCCT GACATCACGT ATGGGCTCCG TGTGCTGGCC 
TTCACCTCTG TTGGAGATGG ACCACCTTCC GATATACTGC AGGTTAAAAC ACAGCAAGGA 
GTCCCTGCTC AGCCGACTGG TTTTGAAGCT GAAGCCGAGT TGGACACGCG GATCATGCTG 
TCATGGTTGT GGCCCGTCCA AGATCAGATC ACCAAATATG AGCTCACATA CTGGGAGGCT 
GACTCTGACA ACAAGCATCA TGTCATCTTT GACCCGGCCG GTTCATATGC TATTGACAGC 
CTAAAGCCAG ACACTCTCTA TAAATTCTCT CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG 
GTTTACACAC AGCCTATCGA GGCCCGCACA GCCCAGTCCG CCCCGTCCGC TCCCCCACAA 
GAGGTGCACC TGATCAGCCT GAGCTCCAGC AGCATCAAGG TGAGTTGGGT GGCCCCTCCG 
GCCGCCAGCC GGCACGGCAG CATCGTCCGC TACTCTCTGG CCTATCAGGC AGTGTCTGGC 
GAGGACCAGG AGCGCCACGA GGTCAAAGAC ATCCCTGCTG ATGTGTCCAG CTATGTTCTG 
GAGGGTCTGG AGAAATGGAC TGAGTATACG GTGTGGGTCA AAGCGCACAC AGATGTGGGT 
CCCGGGCCAG AAAGTGGCAG CACCCGCATC AGAACCCAAG AGGATGTGCC TGGTGCTCCT 
CCACGCAGGG TTGAGGTGGA CAACATCAAC TCCACTGCAC TGCGTGTGTC CTGGAAGCCG 
CCTCTGCAGC AGAAACAACA CGGCCACATA CGTGGATATC AGGTGGTTTA CTCACGCCTG 
GAGAATGGAG AGCCGCGCGG ACAGCCCATC ATTCTGGACG TGGCGCTGCC AGAGGCACAG 
GAGGCCGTGA TCAGTGGTCT TCTCCCTGAA ACCACATATT CAGTGACTGT AGCCGCGTAC 
ACCACTAAAG GAGATGGAGC GCGGAGCAAA GCCAAAGTGG TGACCACAAC AGGAGCAGTT 
CCAGGAAAGC CCACTATGAT CATCAGCACC ACCATAGGCA ACACGGCTCT GATCCAGTGG 
CAGCCGCCTA AAGAGATGGT GGGAGAGCTG ATGGGCTACA GACTCCGCTA TAAGCGTGAA 
GAAGAGGATA TTTACACTGT CCGAGACTTC AGACGCACTG ATGACCACTT CACTGTCACC 
GGCCTGCACA AGGGCGCGAC GTACATCTTC AAACTCTGCG CTAAAAACCG CGCCGGGAAT 
GGAGAGGAGT ACAGCAAAGA GATCAGCACC CCGGAGGATG TGCCCAGCAG TTACCCACAA 
AACCTCAAAG TCGTTGGATT GACCACAACC ACCACAAAAC TAGCCTGGGA TCCTCCGCCG 
CTGCCCGAGA GGAATGGGAA GATCGTACGA TATGTGGTGG TTTATCGAGA CATTAACAGC 
CATCAGAATC AGACCAACGG CACAGCGGAC ACGGAGATGA CCATTCAGGG CCTGAAGCCG 
GACACCACCT ATGATATCAG GGTGAGGGCG TTTACAGGGA AAGGTGGAGG TCCCATCAGC 
CCCAGCATCC AGAGCAGAAC CATGTCTACC TCCATGCCTG AGTTCACGAA AAACTTTGGT 
GTGAAAGCCG TGATGAAGAC CTCTGTCCTG CTCACATGGG AGGTGCCAGA AACATACAAG 
TCTCAAGTGC CTTTCAAGAT CCTGTATAAC CAGCAGAGTG TGGAGGTCCA GGGTGACCTG 
AAGAGGAAGC TGATTACGCG TCTGCAGCCG GACACTGATT ACTCCTTTGT CCTGATGAGT 
CGGGGGAACA GCGCTGGCGG ACTGCAGCAG CAGGTCTCCA TCCGCACCGC TCCAGACCTG 
TTCACTACCA AACCAGCACA CCAGAAACTA GACCCAGAGG AGGGCGGCAA ACTCGCCATC 
ACACTGCCCG AAGTGCACAA CAGCGCCCTC GTCAGGTGGT TTTATATCGT GGTTGTACCA 
GTAACTCCTT CAACTCTCCG CCATTGGGAA AACCCAGAAG ATATTGATCT GGATGAGCTG 
ATGGGGAATA ATAGAGACGC AGCTGTGAGG AGGAGGAGAA GACAAACTCA GGACTTTCTG 
CGGCCTTACA TTGCTGCCAA GCTGGAGTCT CTTCCAGAAA GATTTGTGTT GGGAGATGAG 
AAAACCTACA GCAACTACTA CAACAAACCT CTGCCGGGAC AGCAGCAGTA TCGCACATTT 
ATACTGGCGG CACTGAAAGA CAGAGACTCG ACGTTCACAG TGAGTCCGTT CTCTGAGCCC 
ATGATGGTGA AGCTCCACAG CGGGATGTCC CGTCACGTGG AGGACCCGGA GATGCTGTGG 
GTGATGGGAC CAGTGTTAGC CGTGGTCCTC ATCATCATCA TCGTCATCGC CATCCTCCTC 
TTCAAGAGCA AACAGGAGAG GAAGCGCACG TCTCCCTCAG CTAAAGATGA GCACTCAGCT 
GGAGTTAAAG ATCACCTGCT GGCTCATTCG TCAGATCCAG TGGAGATGAG GAGGCTCAAC 
TACCAGACAC AAGGTTCCAG TGCCTTCAAC TGTCCCAGCA CTTCAAGAAT GCGGGAACAC 
CCTCCAGTGT CTGTGTGTGC TTTGGCTGAC CACATCGAGC GGCTGAGAGC CAACGACAGC 
CTCCGCTTCT CGCAGGAGTA TGAGTCTGTT GACCCTGGCC AGCAGTTCAC CTGGGAAAAC 
TCCAACTTAG AGGTCAACAA GCCCAAGAAC CGCTACGCAA ACGTCATCGC TTACGATCAC 
TCCAGGGTTG TGCTGACTCC TGTGGATGGT GTTCCCGGAA GTGACTACAT CAATGGAAAC 
TACATTGATG GCTACCGTAA GCAGAACGCC TACATCGCCA CGCAGGGTCC GCTGCCTGAG 
ACGCTCAGTG ACTTCTGGAG GATGGTGTGG GAACAGAGAA CCAGCACTAT CGTCATGATG 
ACCCGACTGG AGGAGAAGAG CAGGGTGAAG TGTGATCAGT ACTGGCCAAG CCGAGGAACA 
GAGACTTACG GGATGATTCA AGTGTCCATG CTGGATACGG TGGAGCTGGC CACATACAGC 
GTCAGAACCT TCGCTCTCTA CAAGAATGGA TCCAGCGAGA AACGGGAGGT TCGGCAGTTC 
CAGTTCATGG CGTGGCCGGA TCACGGTGTT CCCGAATATC CCACACCCAT TTTAGCCTTC 
TTACGCAGGG TCAAAGCCTG CAACCCACCT GACGCGGGAC CCATGGTGGT GCACTGCAGC 
GCTGGCGTGG GTCGGACGGG CTGTTTCATC GTAATAGACG CCATGCTGGA GCGGATGAAG 
CACGAGAAGT CGGTGGACAT CTACGGTCAC GTGACCTGCA TGCGTTCTCA GAGGAACTAC 
ATGGTGCAGA CAGAGGATCA GTACATTTTC ATTCACGAGG CCCTGCTGGA GGCTGCCACC 
TGCGGGAACA CAGAGGTGCC GGCGCGGAGC CTCTACACAC ACATCCAGAA ACTCACACAG 
GCGCCGCCTG GAGACACCGT CACCGCCATG GAGCTCGAAT TTAAGAAACT GGCCAACTCT 
AAAGCACACA CCTCCAGATT CATCAGCGCT AGTCTGCCCT GCAACAAATT CAAGAACCGG 
CTGGTGAACA TCATGCCTTT CGAGTCCAGC CGTGTTTGTC TGCAGCCAAT CAGAGGCGTG 
GAGGGCTCCG ACTACATCAA CGCCAGCTTC ATCGACGGAT ACAGGCAGCA GAAGGCATAT 
ATCGCCACTC AAGGCCCGCT GTCTGAAACC ACAGAGGACT TCTGGAGGAT GCTTTGGGAG 
CACAACTCCA CCATCGTGGT GATGCTGACG AAGCTTAGAG AGATGGGACG GGAGAAATGT 
CATCAGTACT GGCCTGCAGA GAGATCTGCG CGCTACCAGT ATTTCGTTGT GGATCCTATG 
GCTGAATATA ACATGCCACA GTACATCTTA CGGGAGTTTA AAGTCACAGA CGCCAGAGAT 
GGCCAGTCTC GGACTATCCG ACAGTTTCAG TTCACAGATT GGCCGGAACA AGGTGTGCCA 
AAGACAGGAG AGGGCTTTAT TGATTTTATT GGTCAAGTCC ACAAAACCAA GGAGCAGTTT 
GGACAGGACG GACCAATTAC TGTTCACTGC AGTGCCGGTG TGGGCAGAAC AGGCGTCTTC 
ATCACTTTAA GTATTGTTCT GGAGCGGATG AGGTATGAAG GAGTGGTGGA CATGTTCCAG 
ACCATAAAAA CACTCCGCAC GCAGAGACCT GCCATGGTAC AGACCGAGGA CCAGTACCAG 
CTGTGCTACA GAGCAGCTCT GGAGTACCTG GGCAGCTTCG ATCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332436.1
CDS	613..6369
Translation	MVPQTRQGSARHGATSLLLLLCLAVPIHADSMPSFVKTPEDQTGISGGVASFVCQAVGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEEDQIPHGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEISWFKDFLPVDINSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAVNSAGTRYSAPANLYVRDQREVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMKGEQELTKEEEMPIGRNVLELTNIRQSGNYTCVAISSLGMIDTTAQITVKALPRPPASLIVTETTATSVTLTWDSGNPEPVSYYVIQYRSKISDNGFQEVDGVASTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSGMVETRTSEQAPSSPPLRVQARMLSATTMLVQWEPPEEPNGQIRGYRVYYTSDLHFPLSTWQKHNTEDSSLTTISGLVPDITYGLRVLAFTSVGDGPPSDILQVKTQQGVPAQPTGFEAEAELDTRIMLSWLWPVQDQITKYELTYWEADSDNKHHVIFDPAGSYAIDSLKPDTLYKFSLAARSEMGLGVYTQPIEARTAQSAPSAPPQEVHLISLSSSSIKVSWVAPPAASRHGSIVRYSLAYQAVSGEDQERHEVKDIPADVSSYVLEGLEKWTEYTVWVKAHTDVGPGPESGSTRIRTQEDVPGAPPRRVEVDNINSTALRVSWKPPLQQKQHGHIRGYQVVYSRLENGEPRGQPIILDVALPEAQEAVISGLLPETTYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTIGNTALIQWQPPKEMVGELMGYRLRYKREEEDIYTVRDFRRTDDHFTVTGLHKGATYIFKLCAKNRAGNGEEYSKEISTPEDVPSSYPQNLKVVGLTTTTTKLAWDPPPLPERNGKIVRYVVVYRDINSHQNQTNGTADTEMTIQGLKPDTTYDIRVRAFTGKGGGPISPSIQSRTMSTSMPEFTKNFGVKAVMKTSVLLTWEVPETYKSQVPFKILYNQQSVEVQGDLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFTTKPAHQKLDPEEGGKLAITLPEVHNSALVRWFYIVVVPVTPSTLRHWENPEDIDLDELMGNNRDAAVRRRRRQTQDFLRPYIAAKLESLPERFVLGDEKTYSNYYNKPLPGQQQYRTFILAALKDRDSTFTVSPFSEPMMVKLHSGMSRHVEDPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRTSPSAKDEHSAGVKDHLLAHSSDPVEMRRLNYQTQGSSAFNCPSTSRMREHPPVSVCALADHIERLRANDSLRFSQEYESVDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINGNYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRTSTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVSMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARSLYTHIQKLTQAPPGDTVTAMELEFKKLANSKAHTSRFISASLPCNKFKNRLVNIMPFESSRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLSETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTIKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021476761.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476761.1

ORF Insert Sequence:

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGACC AGCGGGAAGT CCGACGGGTT 
CCCCCACGTT TTTCAATCCC TCCAACCAAT CACGAGGTGA TGCCGGGTGG CAGTGTGAAC 
CTCACCTGCG TGGCAGTGGG CGCTCCAATG CCCTATGTGA AGTGGATGAA GGGCGAACAG 
GAGCTGACCA AAGAAGAGGA GATGCCCATC GGCCGAAATG TGCTGGAGCT CACCAACATC 
CGCCAGTCGG GGAACTACAC CTGCGTGGCC ATATCATCAC TGGGCATGAT CGACACCACA 
GCGCAGATCA CTGTTAAAGC TCTTCCTCGT CCTCCAGCCT CTCTGATTGT CACTGAAACC 
ACCGCCACCA GTGTGACTTT AACATGGGAT TCTGGCAACC CAGAACCCGT ATCATATTAC 
GTGATCCAGT ATCGATCCAA AATCTCTGAC AATGGTTTCC AGGAGGTGGA CGGCGTGGCA 
TCCACCCGCT ACAGCATCGG CGGGTTGAGT CCGTACTCAG AGTATGAGTT TCGAGTAATG 
GCGGTCAATA ACATTGGTCG CGGGCCGCCC AGCGGGATGG TGGAGACTCG TACCAGTGAA 
CAGGCACCGT CATCTCCTCC GCTGCGCGTT CAAGCCCGGA TGTTGAGCGC TACGACTATG 
CTGGTGCAAT GGGAACCTCC TGAAGAGCCC AACGGACAGA TTCGAGGATA TCGAGTGTAC 
TACACGTCTG ACCTGCACTT CCCGCTCAGC ACATGGCAGA AGCACAACAC TGAGGACAGC 
AGTCTGACCA CCATCTCCGG CCTGGTTCCT GACATCACGT ATGGGCTCCG TGTGCTGGCC 
TTCACCTCTG TTGGAGATGG ACCACCTTCC GATATACTGC AGGTTAAAAC ACAGCAAGGA 
GTCCCTGCTC AGCCGACTGG TTTTGAAGCT GAAGCCGAGT TGGACACGCG GATCATGCTG 
TCATGGTTGT GGCCCGTCCA AGATCAGATC ACCAAATATG AGCTCACATA CTGGGAGGCT 
GACTCTGACA ACAAGCATCA TGTCATCTTT GACCCGGCCG GTTCATATGC TATTGACAGC 
CTAAAGCCAG ACACTCTCTA TAAATTCTCT CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG 
GTTTACACAC AGCCTATCGA GGCCCGCACA GCCCAGTCCG CCCCGTCCGC TCCCCCACAA 
GAGGTGCACC TGATCAGCCT GAGCTCCAGC AGCATCAAGG TGAGTTGGGT GGCCCCTCCG 
GCCGCCAGCC GGCACGGCAG CATCGTCCGC TACTCTCTGG CCTATCAGGC AGTGTCTGGC 
GAGGACCAGG AGCGCCACGA GGTCAAAGAC ATCCCTGCTG ATGTGTCCAG CTATGTTCTG 
GAGGGTCTGG AGAAATGGAC TGAGTATACG GTGTGGGTCA AAGCGCACAC AGATGTGGGT 
CCCGGGCCAG AAAGTGGCAG CACCCGCATC AGAACCCAAG AGGATGTGCC TGGTGCTCCT 
CCACGCAGGG TTGAGGTGGA CAACATCAAC TCCACTGCAC TGCGTGTGTC CTGGAAGCCG 
CCTCTGCAGC AGAAACAACA CGGCCACATA CGTGGATATC AGGTGGTTTA CTCACGCCTG 
GAGAATGGAG AGCCGCGCGG ACAGCCCATC ATTCTGGACG TGGCGCTGCC AGAGGCACAG 
GAGGCCGTGA TCAGTGGTCT TCTCCCTGAA ACCACATATT CAGTGACTGT AGCCGCGTAC 
ACCACTAAAG GAGATGGAGC GCGGAGCAAA GCCAAAGTGG TGACCACAAC AGGAGCAGTT 
CCAGGAAAGC CCACTATGAT CATCAGCACC ACCATAGGCA ACACGGCTCT GATCCAGTGG 
CAGCCGCCTA AAGAGATGGT GGGAGAGCTG ATGGGCTACA GACTCCGCTA TAAGCGTGAA 
GAAGAGGATA TTTACACTGT CCGAGACTTC AGACGCACTG ATGACCACTT CACTGTCACC 
GGCCTGCACA AGGGCGCGAC GTACATCTTC AAACTCTGCG CTAAAAACCG CGCCGGGAAT 
GGAGAGGAGT ACAGCAAAGA GATCAGCACC CCGGAGGATG TGCCCAGCAG TTACCCACAA 
AACCTCAAAG TCGTTGGATT GACCACAACC ACCACAAAAC TAGCCTGGGA TCCTCCGCCG 
CTGCCCGAGA GGAATGGGAA GATCGTACGA TATGTGGTGG TTTATCGAGA CATTAACAGC 
CATCAGAATC AGACCAACGG CACAGCGGAC ACGGAGATGA CCATTCAGGG CCTGAAGCCG 
GACACCACCT ATGATATCAG GGTGAGGGCG TTTACAGGGA AAGGTGGAGG TCCCATCAGC 
CCCAGCATCC AGAGCAGAAC CATGTCTACC TCCATGCCTG AGTTCACGAA AAACTTTGGT 
GTGAAAGCCG TGATGAAGAC CTCTGTCCTG CTCACATGGG AGGTGCCAGA AACATACAAG 
TCTCAAGTGC CTTTCAAGAT CCTGTATAAC CAGCAGAGTG TGGAGGTCCA GGGTGACCTG 
AAGAGGAAGC TGATTACGCG TCTGCAGCCG GACACTGATT ACTCCTTTGT CCTGATGAGT 
CGGGGGAACA GCGCTGGCGG ACTGCAGCAG CAGGTCTCCA TCCGCACCGC TCCAGACCTG 
TTCACTACCA AACCAGCACA CCAGAAACTA GACCCAGAGG AGGGCGGCAA ACTCGCCATC 
ACACTGCCCG AAGTGCACAA CAGCGCCCTC GTCAGGTGGT TTTATATCGT GGTTGTACCA 
GTAACTCCTT CAACTCTCCG CCATTGGGAA AACCCAGAAG ATATTGATCT GGATGAGCTG 
ATGGGGAATA ATAGAGACGC AGCTGTGAGG AGGAGGAGAA GACAAACTCA GGACTTTCTG 
CGGCCTTACA TTGCTGCCAA GCTGGAGTCT CTTCCAGAAA GATTTGTGTT GGGAGATGAG 
AAAACCTACA GCAACTACTA CAACAAACCT CTGCCGGGAC AGCAGCAGTA TCGCACATTT 
ATACTGGCGG CACTGAAAGA CAGAGACTCG ACGTTCACAG TGAGTCCGTT CTCTGAGCCC 
ATGATGGTGA AGCTCCACAG CGGGATGTCC CGTCACGTGG AGGACCCGGA GATGCTGTGG 
GTGATGGGAC CAGTGTTAGC CGTGGTCCTC ATCATCATCA TCGTCATCGC CATCCTCCTC 
TTCAAGAGCA AACAGGAGAG GAAGCGCACG TCTCCCTCAG CTAAAGATGA GCACTCAGCT 
GGAGTTAAAG ATCACCTGCT GGCTCATTCG TCAGATCCAG TGGAGATGAG GAGGCTCAAC 
TACCAGACAC AAGGTTCCAG TGCCTTCAAC TGTCCCAGCA CTTCAAGAAT GCGGGAACAC 
CCTCCAGTGT CTGTGTGTGC TTTGGCTGAC CACATCGAGC GGCTGAGAGC CAACGACAGC 
CTCCGCTTCT CGCAGGAGTA TGAGTCTGTT GACCCTGGCC AGCAGTTCAC CTGGGAAAAC 
TCCAACTTAG AGGTCAACAA GCCCAAGAAC CGCTACGCAA ACGTCATCGC TTACGATCAC 
TCCAGGGTTG TGCTGACTCC TGTGGATGGT GTTCCCGGAA GTGACTACAT CAATGGAAAC 
TACATTGATG GCTACCGTAA GCAGAACGCC TACATCGCCA CGCAGGGTCC GCTGCCTGAG 
ACGCTCAGTG ACTTCTGGAG GATGGTGTGG GAACAGAGAA CCAGCACTAT CGTCATGATG 
ACCCGACTGG AGGAGAAGAG CAGGGTGAAG TGTGATCAGT ACTGGCCAAG CCGAGGAACA 
GAGACTTACG GGATGATTCA AGTGTCCATG CTGGATACGG TGGAGCTGGC CACATACAGC 
GTCAGAACCT TCGCTCTCTA CAAGAATGGA TCCAGCGAGA AACGGGAGGT TCGGCAGTTC 
CAGTTCATGG CGTGGCCGGA TCACGGTGTT CCCGAATATC CCACACCCAT TTTAGCCTTC 
TTACGCAGGG TCAAAGCCTG CAACCCACCT GACGCGGGAC CCATGGTGGT GCACTGCAGC 
GCTGGCGTGG GTCGGACGGG CTGTTTCATC GTAATAGACG CCATGCTGGA GCGGATGAAG 
CACGAGAAGT CGGTGGACAT CTACGGTCAC GTGACCTGCA TGCGTTCTCA GAGGAACTAC 
ATGGTGCAGA CAGAGGATCA GTACATTTTC ATTCACGAGG CCCTGCTGGA GGCTGCCACC 
TGCGGGAACA CAGAGGTGCC GGCGCGGAGC CTCTACACAC ACATCCAGAA ACTCACACAG 
GCGCCGCCTG GAGACACCGT CACCGCCATG GAGCTCGAAT TTAAGAAACT GGCCAACTCT 
AAAGCACACA CCTCCAGATT CATCAGCGCT AGTCTGCCCT GCAACAAATT CAAGAACCGG 
CTGGTGAACA TCATGCCTTT CGAGTCCAGC CGTGTTTGTC TGCAGCCAAT CAGAGGCGTG 
GAGGGCTCCG ACTACATCAA CGCCAGCTTC ATCGACGGAT ACAGGCAGCA GAAGGCATAT 
ATCGCCACTC AAGGCCCGCT GTCTGAAACC ACAGAGGACT TCTGGAGGAT GCTTTGGGAG 
CACAACTCCA CCATCGTGGT GATGCTGACG AAGCTTAGAG AGATGGGACG GGAGAAATGT 
CATCAGTACT GGCCTGCAGA GAGATCTGCG CGCTACCAGT ATTTCGTTGT GGATCCTATG 
GCTGAATATA ACATGCCACA GTACATCTTA CGGGAGTTTA AAGTCACAGA CGCCAGAGAT 
GGCCAGTCTC GGACTATCCG ACAGTTTCAG TTCACAGATT GGCCGGAACA AGGTGTGCCA 
AAGACAGGAG AGGGCTTTAT TGATTTTATT GGTCAAGTCC ACAAAACCAA GGAGCAGTTT 
GGACAGGACG GACCAATTAC TGTTCACTGC AGTGCCGGTG TGGGCAGAAC AGGCGTCTTC 
ATCACTTTAA GTATTGTTCT GGAGCGGATG AGGTATGAAG GAGTGGTGGA CATGTTCCAG 
ACCATAAAAA CACTCCGCAC GCAGAGACCT GCCATGGTAC AGACCGAGGA CCAGTACCAG 
CTGTGCTACA GAGCAGCTCT GGAGTACCTG GGCAGCTTCG ATCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfa ( XM_021476762.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332437.1 Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X3, mRNA.

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CloneID	ODa142903
Clone ID Related Accession (Same CDS sequence)	XM_021476762.1
Accession Version	XM_021476762.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGTCC GACGGGTTCC CCCACGTTTT 
TCAATCCCTC CAACCAATCA CGAGGTGATG CCGGGTGGCA GTGTGAACCT CACCTGCGTG 
GCAGTGGGCG CTCCAATGCC CTATGTGAAG TGGATGAAGG GCGAACAGGA GCTGACCAAA 
GAAGAGGAGA TGCCCATCGG CCGAAATGTG CTGGAGCTCA CCAACATCCG CCAGTCGGGG 
AACTACACCT GCGTGGCCAT ATCATCACTG GGCATGATCG ACACCACAGC GCAGATCACT 
GTTAAAGCTC TTCCTCGTCC TCCAGCCTCT CTGATTGTCA CTGAAACCAC CGCCACCAGT 
GTGACTTTAA CATGGGATTC TGGCAACCCA GAACCCGTAT CATATTACGT GATCCAGTAT 
CGATCCAAAA TCTCTGACAA TGGTTTCCAG GAGGTGGACG GCGTGGCATC CACCCGCTAC 
AGCATCGGCG GGTTGAGTCC GTACTCAGAG TATGAGTTTC GAGTAATGGC GGTCAATAAC 
ATTGGTCGCG GGCCGCCCAG CGGGATGGTG GAGACTCGTA CCAGTGAACA GGCACCGTCA 
TCTCCTCCGC TGCGCGTTCA AGCCCGGATG TTGAGCGCTA CGACTATGCT GGTGCAATGG 
GAACCTCCTG AAGAGCCCAA CGGACAGATT CGAGGATATC GAGTGTACTA CACGTCTGAC 
CTGCACTTCC CGCTCAGCAC ATGGCAGAAG CACAACACTG AGGACAGCAG TCTGACCACC 
ATCTCCGGCC TGGTTCCTGA CATCACGTAT GGGCTCCGTG TGCTGGCCTT CACCTCTGTT 
GGAGATGGAC CACCTTCCGA TATACTGCAG GTTAAAACAC AGCAAGGAGT CCCTGCTCAG 
CCGACTGGTT TTGAAGCTGA AGCCGAGTTG GACACGCGGA TCATGCTGTC ATGGTTGTGG 
CCCGTCCAAG ATCAGATCAC CAAATATGAG CTCACATACT GGGAGGCTGA CTCTGACAAC 
AAGCATCATG TCATCTTTGA CCCGGCCGGT TCATATGCTA TTGACAGCCT AAAGCCAGAC 
ACTCTCTATA AATTCTCTCT GGCGGCGCGC TCTGAGATGG GGCTCGGGGT TTACACACAG 
CCTATCGAGG CCCGCACAGC CCAGTCCGCC CCGTCCGCTC CCCCACAAGA GGTGCACCTG 
ATCAGCCTGA GCTCCAGCAG CATCAAGGTG AGTTGGGTGG CCCCTCCGGC CGCCAGCCGG 
CACGGCAGCA TCGTCCGCTA CTCTCTGGCC TATCAGGCAG TGTCTGGCGA GGACCAGGAG 
CGCCACGAGG TCAAAGACAT CCCTGCTGAT GTGTCCAGCT ATGTTCTGGA GGGTCTGGAG 
AAATGGACTG AGTATACGGT GTGGGTCAAA GCGCACACAG ATGTGGGTCC CGGGCCAGAA 
AGTGGCAGCA CCCGCATCAG AACCCAAGAG GATGTGCCTG GTGCTCCTCC ACGCAGGGTT 
GAGGTGGACA ACATCAACTC CACTGCACTG CGTGTGTCCT GGAAGCCGCC TCTGCAGCAG 
AAACAACACG GCCACATACG TGGATATCAG GTGGTTTACT CACGCCTGGA GAATGGAGAG 
CCGCGCGGAC AGCCCATCAT TCTGGACGTG GCGCTGCCAG AGGCACAGGA GGCCGTGATC 
AGTGGTCTTC TCCCTGAAAC CACATATTCA GTGACTGTAG CCGCGTACAC CACTAAAGGA 
GATGGAGCGC GGAGCAAAGC CAAAGTGGTG ACCACAACAG GAGCAGTTCC AGGAAAGCCC 
ACTATGATCA TCAGCACCAC CATAGGCAAC ACGGCTCTGA TCCAGTGGCA GCCGCCTAAA 
GAGATGGTGG GAGAGCTGAT GGGCTACAGA CTCCGCTATA AGCGTGAAGA AGAGGATATT 
TACACTGTCC GAGACTTCAG ACGCACTGAT GACCACTTCA CTGTCACCGG CCTGCACAAG 
GGCGCGACGT ACATCTTCAA ACTCTGCGCT AAAAACCGCG CCGGGAATGG AGAGGAGTAC 
AGCAAAGAGA TCAGCACCCC GGAGGATGTG CCCAGCAGTT ACCCACAAAA CCTCAAAGTC 
GTTGGATTGA CCACAACCAC CACAAAACTA GCCTGGGATC CTCCGCCGCT GCCCGAGAGG 
AATGGGAAGA TCGTACGATA TGTGGTGGTT TATCGAGACA TTAACAGCCA TCAGAATCAG 
ACCAACGGCA CAGCGGACAC GGAGATGACC ATTCAGGGCC TGAAGCCGGA CACCACCTAT 
GATATCAGGG TGAGGGCGTT TACAGGGAAA GGTGGAGGTC CCATCAGCCC CAGCATCCAG 
AGCAGAACCA TGTCTACCTC CATGCCTGAG TTCACGAAAA ACTTTGGTGT GAAAGCCGTG 
ATGAAGACCT CTGTCCTGCT CACATGGGAG GTGCCAGAAA CATACAAGTC TCAAGTGCCT 
TTCAAGATCC TGTATAACCA GCAGAGTGTG GAGGTCCAGG GTGACCTGAA GAGGAAGCTG 
ATTACGCGTC TGCAGCCGGA CACTGATTAC TCCTTTGTCC TGATGAGTCG GGGGAACAGC 
GCTGGCGGAC TGCAGCAGCA GGTCTCCATC CGCACCGCTC CAGACCTGTT CACTACCAAA 
CCAGCACACC AGAAACTAGA CCCAGAGGAG GGCGGCAAAC TCGCCATCAC ACTGCCCGAA 
GTGCACAACA GCGCCCTCGT CAGGTGGTTT TATATCGTGG TTGTACCAGT AACTCCTTCA 
ACTCTCCGCC ATTGGGAAAA CCCAGAAGAT ATTGATCTGG ATGAGCTGAT GGGGAATAAT 
AGAGACGCAG CTGTGAGGAG GAGGAGAAGA CAAACTCAGG ACTTTCTGCG GCCTTACATT 
GCTGCCAAGC TGGAGTCTCT TCCAGAAAGA TTTGTGTTGG GAGATGAGAA AACCTACAGC 
AACTACTACA ACAAACCTCT GCCGGGACAG CAGCAGTATC GCACATTTAT ACTGGCGGCA 
CTGAAAGACA GAGACTCGAC GTTCACAGTG AGTCCGTTCT CTGAGCCCAT GATGGTGAAG 
CTCCACAGCG GGATGTCCCG TCACGTGGAG GACCCGGAGA TGCTGTGGGT GATGGGACCA 
GTGTTAGCCG TGGTCCTCAT CATCATCATC GTCATCGCCA TCCTCCTCTT CAAGAGCAAA 
CAGGAGAGGA AGCGCACGTC TCCCTCAGCT AAAGATGAGC ACTCAGCTGG AGTTAAAGAT 
CACCTGCTGG CTCATTCGTC AGATCCAGTG GAGATGAGGA GGCTCAACTA CCAGACACAA 
GGTTCCAGTG CCTTCAACTG TCCCAGCACT TCAAGAATGC GGGAACACCC TCCAGTGTCT 
GTGTGTGCTT TGGCTGACCA CATCGAGCGG CTGAGAGCCA ACGACAGCCT CCGCTTCTCG 
CAGGAGTATG AGTCTGTTGA CCCTGGCCAG CAGTTCACCT GGGAAAACTC CAACTTAGAG 
GTCAACAAGC CCAAGAACCG CTACGCAAAC GTCATCGCTT ACGATCACTC CAGGGTTGTG 
CTGACTCCTG TGGATGGTGT TCCCGGAAGT GACTACATCA ATGGAAACTA CATTGATGGC 
TACCGTAAGC AGAACGCCTA CATCGCCACG CAGGGTCCGC TGCCTGAGAC GCTCAGTGAC 
TTCTGGAGGA TGGTGTGGGA ACAGAGAACC AGCACTATCG TCATGATGAC CCGACTGGAG 
GAGAAGAGCA GGGTGAAGTG TGATCAGTAC TGGCCAAGCC GAGGAACAGA GACTTACGGG 
ATGATTCAAG TGTCCATGCT GGATACGGTG GAGCTGGCCA CATACAGCGT CAGAACCTTC 
GCTCTCTACA AGAATGGATC CAGCGAGAAA CGGGAGGTTC GGCAGTTCCA GTTCATGGCG 
TGGCCGGATC ACGGTGTTCC CGAATATCCC ACACCCATTT TAGCCTTCTT ACGCAGGGTC 
AAAGCCTGCA ACCCACCTGA CGCGGGACCC ATGGTGGTGC ACTGCAGCGC TGGCGTGGGT 
CGGACGGGCT GTTTCATCGT AATAGACGCC ATGCTGGAGC GGATGAAGCA CGAGAAGTCG 
GTGGACATCT ACGGTCACGT GACCTGCATG CGTTCTCAGA GGAACTACAT GGTGCAGACA 
GAGGATCAGT ACATTTTCAT TCACGAGGCC CTGCTGGAGG CTGCCACCTG CGGGAACACA 
GAGGTGCCGG CGCGGAGCCT CTACACACAC ATCCAGAAAC TCACACAGGC GCCGCCTGGA 
GACACCGTCA CCGCCATGGA GCTCGAATTT AAGAAACTGG CCAACTCTAA AGCACACACC 
TCCAGATTCA TCAGCGCTAG TCTGCCCTGC AACAAATTCA AGAACCGGCT GGTGAACATC 
ATGCCTTTCG AGTCCAGCCG TGTTTGTCTG CAGCCAATCA GAGGCGTGGA GGGCTCCGAC 
TACATCAACG CCAGCTTCAT CGACGGATAC AGGCAGCAGA AGGCATATAT CGCCACTCAA 
GGCCCGCTGT CTGAAACCAC AGAGGACTTC TGGAGGATGC TTTGGGAGCA CAACTCCACC 
ATCGTGGTGA TGCTGACGAA GCTTAGAGAG ATGGGACGGG AGAAATGTCA TCAGTACTGG 
CCTGCAGAGA GATCTGCGCG CTACCAGTAT TTCGTTGTGG ATCCTATGGC TGAATATAAC 
ATGCCACAGT ACATCTTACG GGAGTTTAAA GTCACAGACG CCAGAGATGG CCAGTCTCGG 
ACTATCCGAC AGTTTCAGTT CACAGATTGG CCGGAACAAG GTGTGCCAAA GACAGGAGAG 
GGCTTTATTG ATTTTATTGG TCAAGTCCAC AAAACCAAGG AGCAGTTTGG ACAGGACGGA 
CCAATTACTG TTCACTGCAG TGCCGGTGTG GGCAGAACAG GCGTCTTCAT CACTTTAAGT 
ATTGTTCTGG AGCGGATGAG GTATGAAGGA GTGGTGGACA TGTTCCAGAC CATAAAAACA 
CTCCGCACGC AGAGACCTGC CATGGTACAG ACCGAGGACC AGTACCAGCT GTGCTACAGA 
GCAGCTCTGG AGTACCTGGG CAGCTTCGAT CACTATGCAA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332437.1
CDS	1458..7202
Translation	MVPQTRQGSARHGATSLLLLLCLAVPIHADSMPSFVKTPEDQTGISGGVASFVCQAVGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEEDQIPHGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEISWFKDFLPVDINSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAVNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMKGEQELTKEEEMPIGRNVLELTNIRQSGNYTCVAISSLGMIDTTAQITVKALPRPPASLIVTETTATSVTLTWDSGNPEPVSYYVIQYRSKISDNGFQEVDGVASTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSGMVETRTSEQAPSSPPLRVQARMLSATTMLVQWEPPEEPNGQIRGYRVYYTSDLHFPLSTWQKHNTEDSSLTTISGLVPDITYGLRVLAFTSVGDGPPSDILQVKTQQGVPAQPTGFEAEAELDTRIMLSWLWPVQDQITKYELTYWEADSDNKHHVIFDPAGSYAIDSLKPDTLYKFSLAARSEMGLGVYTQPIEARTAQSAPSAPPQEVHLISLSSSSIKVSWVAPPAASRHGSIVRYSLAYQAVSGEDQERHEVKDIPADVSSYVLEGLEKWTEYTVWVKAHTDVGPGPESGSTRIRTQEDVPGAPPRRVEVDNINSTALRVSWKPPLQQKQHGHIRGYQVVYSRLENGEPRGQPIILDVALPEAQEAVISGLLPETTYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTIGNTALIQWQPPKEMVGELMGYRLRYKREEEDIYTVRDFRRTDDHFTVTGLHKGATYIFKLCAKNRAGNGEEYSKEISTPEDVPSSYPQNLKVVGLTTTTTKLAWDPPPLPERNGKIVRYVVVYRDINSHQNQTNGTADTEMTIQGLKPDTTYDIRVRAFTGKGGGPISPSIQSRTMSTSMPEFTKNFGVKAVMKTSVLLTWEVPETYKSQVPFKILYNQQSVEVQGDLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFTTKPAHQKLDPEEGGKLAITLPEVHNSALVRWFYIVVVPVTPSTLRHWENPEDIDLDELMGNNRDAAVRRRRRQTQDFLRPYIAAKLESLPERFVLGDEKTYSNYYNKPLPGQQQYRTFILAALKDRDSTFTVSPFSEPMMVKLHSGMSRHVEDPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRTSPSAKDEHSAGVKDHLLAHSSDPVEMRRLNYQTQGSSAFNCPSTSRMREHPPVSVCALADHIERLRANDSLRFSQEYESVDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINGNYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRTSTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVSMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARSLYTHIQKLTQAPPGDTVTAMELEFKKLANSKAHTSRFISASLPCNKFKNRLVNIMPFESSRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLSETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTIKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021476762.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476762.1

ORF Insert Sequence:

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGTCC GACGGGTTCC CCCACGTTTT 
TCAATCCCTC CAACCAATCA CGAGGTGATG CCGGGTGGCA GTGTGAACCT CACCTGCGTG 
GCAGTGGGCG CTCCAATGCC CTATGTGAAG TGGATGAAGG GCGAACAGGA GCTGACCAAA 
GAAGAGGAGA TGCCCATCGG CCGAAATGTG CTGGAGCTCA CCAACATCCG CCAGTCGGGG 
AACTACACCT GCGTGGCCAT ATCATCACTG GGCATGATCG ACACCACAGC GCAGATCACT 
GTTAAAGCTC TTCCTCGTCC TCCAGCCTCT CTGATTGTCA CTGAAACCAC CGCCACCAGT 
GTGACTTTAA CATGGGATTC TGGCAACCCA GAACCCGTAT CATATTACGT GATCCAGTAT 
CGATCCAAAA TCTCTGACAA TGGTTTCCAG GAGGTGGACG GCGTGGCATC CACCCGCTAC 
AGCATCGGCG GGTTGAGTCC GTACTCAGAG TATGAGTTTC GAGTAATGGC GGTCAATAAC 
ATTGGTCGCG GGCCGCCCAG CGGGATGGTG GAGACTCGTA CCAGTGAACA GGCACCGTCA 
TCTCCTCCGC TGCGCGTTCA AGCCCGGATG TTGAGCGCTA CGACTATGCT GGTGCAATGG 
GAACCTCCTG AAGAGCCCAA CGGACAGATT CGAGGATATC GAGTGTACTA CACGTCTGAC 
CTGCACTTCC CGCTCAGCAC ATGGCAGAAG CACAACACTG AGGACAGCAG TCTGACCACC 
ATCTCCGGCC TGGTTCCTGA CATCACGTAT GGGCTCCGTG TGCTGGCCTT CACCTCTGTT 
GGAGATGGAC CACCTTCCGA TATACTGCAG GTTAAAACAC AGCAAGGAGT CCCTGCTCAG 
CCGACTGGTT TTGAAGCTGA AGCCGAGTTG GACACGCGGA TCATGCTGTC ATGGTTGTGG 
CCCGTCCAAG ATCAGATCAC CAAATATGAG CTCACATACT GGGAGGCTGA CTCTGACAAC 
AAGCATCATG TCATCTTTGA CCCGGCCGGT TCATATGCTA TTGACAGCCT AAAGCCAGAC 
ACTCTCTATA AATTCTCTCT GGCGGCGCGC TCTGAGATGG GGCTCGGGGT TTACACACAG 
CCTATCGAGG CCCGCACAGC CCAGTCCGCC CCGTCCGCTC CCCCACAAGA GGTGCACCTG 
ATCAGCCTGA GCTCCAGCAG CATCAAGGTG AGTTGGGTGG CCCCTCCGGC CGCCAGCCGG 
CACGGCAGCA TCGTCCGCTA CTCTCTGGCC TATCAGGCAG TGTCTGGCGA GGACCAGGAG 
CGCCACGAGG TCAAAGACAT CCCTGCTGAT GTGTCCAGCT ATGTTCTGGA GGGTCTGGAG 
AAATGGACTG AGTATACGGT GTGGGTCAAA GCGCACACAG ATGTGGGTCC CGGGCCAGAA 
AGTGGCAGCA CCCGCATCAG AACCCAAGAG GATGTGCCTG GTGCTCCTCC ACGCAGGGTT 
GAGGTGGACA ACATCAACTC CACTGCACTG CGTGTGTCCT GGAAGCCGCC TCTGCAGCAG 
AAACAACACG GCCACATACG TGGATATCAG GTGGTTTACT CACGCCTGGA GAATGGAGAG 
CCGCGCGGAC AGCCCATCAT TCTGGACGTG GCGCTGCCAG AGGCACAGGA GGCCGTGATC 
AGTGGTCTTC TCCCTGAAAC CACATATTCA GTGACTGTAG CCGCGTACAC CACTAAAGGA 
GATGGAGCGC GGAGCAAAGC CAAAGTGGTG ACCACAACAG GAGCAGTTCC AGGAAAGCCC 
ACTATGATCA TCAGCACCAC CATAGGCAAC ACGGCTCTGA TCCAGTGGCA GCCGCCTAAA 
GAGATGGTGG GAGAGCTGAT GGGCTACAGA CTCCGCTATA AGCGTGAAGA AGAGGATATT 
TACACTGTCC GAGACTTCAG ACGCACTGAT GACCACTTCA CTGTCACCGG CCTGCACAAG 
GGCGCGACGT ACATCTTCAA ACTCTGCGCT AAAAACCGCG CCGGGAATGG AGAGGAGTAC 
AGCAAAGAGA TCAGCACCCC GGAGGATGTG CCCAGCAGTT ACCCACAAAA CCTCAAAGTC 
GTTGGATTGA CCACAACCAC CACAAAACTA GCCTGGGATC CTCCGCCGCT GCCCGAGAGG 
AATGGGAAGA TCGTACGATA TGTGGTGGTT TATCGAGACA TTAACAGCCA TCAGAATCAG 
ACCAACGGCA CAGCGGACAC GGAGATGACC ATTCAGGGCC TGAAGCCGGA CACCACCTAT 
GATATCAGGG TGAGGGCGTT TACAGGGAAA GGTGGAGGTC CCATCAGCCC CAGCATCCAG 
AGCAGAACCA TGTCTACCTC CATGCCTGAG TTCACGAAAA ACTTTGGTGT GAAAGCCGTG 
ATGAAGACCT CTGTCCTGCT CACATGGGAG GTGCCAGAAA CATACAAGTC TCAAGTGCCT 
TTCAAGATCC TGTATAACCA GCAGAGTGTG GAGGTCCAGG GTGACCTGAA GAGGAAGCTG 
ATTACGCGTC TGCAGCCGGA CACTGATTAC TCCTTTGTCC TGATGAGTCG GGGGAACAGC 
GCTGGCGGAC TGCAGCAGCA GGTCTCCATC CGCACCGCTC CAGACCTGTT CACTACCAAA 
CCAGCACACC AGAAACTAGA CCCAGAGGAG GGCGGCAAAC TCGCCATCAC ACTGCCCGAA 
GTGCACAACA GCGCCCTCGT CAGGTGGTTT TATATCGTGG TTGTACCAGT AACTCCTTCA 
ACTCTCCGCC ATTGGGAAAA CCCAGAAGAT ATTGATCTGG ATGAGCTGAT GGGGAATAAT 
AGAGACGCAG CTGTGAGGAG GAGGAGAAGA CAAACTCAGG ACTTTCTGCG GCCTTACATT 
GCTGCCAAGC TGGAGTCTCT TCCAGAAAGA TTTGTGTTGG GAGATGAGAA AACCTACAGC 
AACTACTACA ACAAACCTCT GCCGGGACAG CAGCAGTATC GCACATTTAT ACTGGCGGCA 
CTGAAAGACA GAGACTCGAC GTTCACAGTG AGTCCGTTCT CTGAGCCCAT GATGGTGAAG 
CTCCACAGCG GGATGTCCCG TCACGTGGAG GACCCGGAGA TGCTGTGGGT GATGGGACCA 
GTGTTAGCCG TGGTCCTCAT CATCATCATC GTCATCGCCA TCCTCCTCTT CAAGAGCAAA 
CAGGAGAGGA AGCGCACGTC TCCCTCAGCT AAAGATGAGC ACTCAGCTGG AGTTAAAGAT 
CACCTGCTGG CTCATTCGTC AGATCCAGTG GAGATGAGGA GGCTCAACTA CCAGACACAA 
GGTTCCAGTG CCTTCAACTG TCCCAGCACT TCAAGAATGC GGGAACACCC TCCAGTGTCT 
GTGTGTGCTT TGGCTGACCA CATCGAGCGG CTGAGAGCCA ACGACAGCCT CCGCTTCTCG 
CAGGAGTATG AGTCTGTTGA CCCTGGCCAG CAGTTCACCT GGGAAAACTC CAACTTAGAG 
GTCAACAAGC CCAAGAACCG CTACGCAAAC GTCATCGCTT ACGATCACTC CAGGGTTGTG 
CTGACTCCTG TGGATGGTGT TCCCGGAAGT GACTACATCA ATGGAAACTA CATTGATGGC 
TACCGTAAGC AGAACGCCTA CATCGCCACG CAGGGTCCGC TGCCTGAGAC GCTCAGTGAC 
TTCTGGAGGA TGGTGTGGGA ACAGAGAACC AGCACTATCG TCATGATGAC CCGACTGGAG 
GAGAAGAGCA GGGTGAAGTG TGATCAGTAC TGGCCAAGCC GAGGAACAGA GACTTACGGG 
ATGATTCAAG TGTCCATGCT GGATACGGTG GAGCTGGCCA CATACAGCGT CAGAACCTTC 
GCTCTCTACA AGAATGGATC CAGCGAGAAA CGGGAGGTTC GGCAGTTCCA GTTCATGGCG 
TGGCCGGATC ACGGTGTTCC CGAATATCCC ACACCCATTT TAGCCTTCTT ACGCAGGGTC 
AAAGCCTGCA ACCCACCTGA CGCGGGACCC ATGGTGGTGC ACTGCAGCGC TGGCGTGGGT 
CGGACGGGCT GTTTCATCGT AATAGACGCC ATGCTGGAGC GGATGAAGCA CGAGAAGTCG 
GTGGACATCT ACGGTCACGT GACCTGCATG CGTTCTCAGA GGAACTACAT GGTGCAGACA 
GAGGATCAGT ACATTTTCAT TCACGAGGCC CTGCTGGAGG CTGCCACCTG CGGGAACACA 
GAGGTGCCGG CGCGGAGCCT CTACACACAC ATCCAGAAAC TCACACAGGC GCCGCCTGGA 
GACACCGTCA CCGCCATGGA GCTCGAATTT AAGAAACTGG CCAACTCTAA AGCACACACC 
TCCAGATTCA TCAGCGCTAG TCTGCCCTGC AACAAATTCA AGAACCGGCT GGTGAACATC 
ATGCCTTTCG AGTCCAGCCG TGTTTGTCTG CAGCCAATCA GAGGCGTGGA GGGCTCCGAC 
TACATCAACG CCAGCTTCAT CGACGGATAC AGGCAGCAGA AGGCATATAT CGCCACTCAA 
GGCCCGCTGT CTGAAACCAC AGAGGACTTC TGGAGGATGC TTTGGGAGCA CAACTCCACC 
ATCGTGGTGA TGCTGACGAA GCTTAGAGAG ATGGGACGGG AGAAATGTCA TCAGTACTGG 
CCTGCAGAGA GATCTGCGCG CTACCAGTAT TTCGTTGTGG ATCCTATGGC TGAATATAAC 
ATGCCACAGT ACATCTTACG GGAGTTTAAA GTCACAGACG CCAGAGATGG CCAGTCTCGG 
ACTATCCGAC AGTTTCAGTT CACAGATTGG CCGGAACAAG GTGTGCCAAA GACAGGAGAG 
GGCTTTATTG ATTTTATTGG TCAAGTCCAC AAAACCAAGG AGCAGTTTGG ACAGGACGGA 
CCAATTACTG TTCACTGCAG TGCCGGTGTG GGCAGAACAG GCGTCTTCAT CACTTTAAGT 
ATTGTTCTGG AGCGGATGAG GTATGAAGGA GTGGTGGACA TGTTCCAGAC CATAAAAACA 
CTCCGCACGC AGAGACCTGC CATGGTACAG ACCGAGGACC AGTACCAGCT GTGCTACAGA 
GCAGCTCTGG AGTACCTGGG CAGCTTCGAT CACTATGCAA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfa ( XM_021476763.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332438.1 Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X4, mRNA.

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CloneID	ODa142904
Clone ID Related Accession (Same CDS sequence)	XM_021476763.1
Accession Version	XM_021476763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGACC AGCGGGAAGT CCGACGGGTT 
CCCCCACGTT TTTCAATCCC TCCAACCAAT CACGAGGTGA TGCCGGGTGG CAGTGTGAAC 
CTCACCTGCG TGGCAGTGGG CGCTCCAATG CCCTATGTGA AGTGGATGAA GGGCGAACAG 
GAGCTGACCA AAGAAGAGGA GATGCCCATC GGCCGAAATG TGCTGGAGCT CACCAACATC 
CGCCAGTCGG GGAACTACAC CTGCGTGGCC ATATCATCAC TGGGCATGAT CGACACCACA 
GCGCAGATCA CTGTTAAAGC TCTTCCTCGT CCTCCAGCCT CTCTGATTGT CACTGAAACC 
ACCGCCACCA GTGTGACTTT AACATGGGAT TCTGGCAACC CAGAACCCGT ATCATATTAC 
GTGATCCAGT ATCGATCCAA AATCTCTGAC AATGGTTTCC AGGAGGTGGA CGGCGTGGCA 
TCCACCCGCT ACAGCATCGG CGGGTTGAGT CCGTACTCAG AGTATGAGTT TCGAGTAATG 
GCGGTCAATA ACATTGGTCG CGGGCCGCCC AGCGGGATGG TGGAGACTCG TACCAGTGAA 
CAGGCACCGT CATCTCCTCC GCTGCGCGTT CAAGCCCGGA TGTTGAGCGC TACGACTATG 
CTGGTGCAAT GGGAACCTCC TGAAGAGCCC AACGGACAGA TTCGAGGATA TCGAGTGTAC 
TACACGTCTG ACCTGCACTT CCCGCTCAGC ACATGGCAGA AGCACAACAC TGAGGACAGC 
AGTCTGACCA CCATCTCCGG CCTGGTTCCT GACATCACGT ATGGGCTCCG TGTGCTGGCC 
TTCACCTCTG TTGGAGATGG ACCACCTTCC GATATACTGC AGGTTAAAAC ACAGCAAGGA 
GTCCCTGCTC AGCCGACTGG TTTTGAAGCT GAAGCCGAGT TGGACACGCG GATCATGCTG 
TCATGGTTGT GGCCCGTCCA AGATCAGATC ACCAAATATG AGCTCACATA CTGGGAGGCT 
GACTCTGACA ACAAGCATCA TGTCATCTTT GACCCGGCCG GTTCATATGC TATTGACAGC 
CTAAAGCCAG ACACTCTCTA TAAATTCTCT CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG 
GTTTACACAC AGCCTATCGA GGCCCGCACA GCCCAGTCCG CCCCGTCCGC TCCCCCACAA 
GAGGTGCACC TGATCAGCCT GAGCTCCAGC AGCATCAAGG TGAGTTGGGT GGCCCCTCCG 
GCCGCCAGCC GGCACGGCAG CATCGTCCGC TACTCTCTGG CCTATCAGGC AGTGTCTGGC 
GAGGACCAGG AGCGCCACGA GGTCAAAGAC ATCCCTGCTG ATGTGTCCAG CTATGTTCTG 
GAGGGTCTGG AGAAATGGAC TGAGTATACG GTGTGGGTCA AAGCGCACAC AGATGTGGGT 
CCCGGGCCAG AAAGTGGCAG CACCCGCATC AGAACCCAAG AGGATGTGCC TGGTGCTCCT 
CCACGCAGGG TTGAGGTGGA CAACATCAAC TCCACTGCAC TGCGTGTGTC CTGGAAGCCG 
CCTCTGCAGC AGAAACAACA CGGCCACATA CGTGGATATC AGGTGGTTTA CTCACGCCTG 
GAGAATGGAG AGCCGCGCGG ACAGCCCATC ATTCTGGACG TGGCGCTGCC AGAGGCACAG 
GAGGCCGTGA TCAGTGGTCT TCTCCCTGAA ACCACATATT CAGTGACTGT AGCCGCGTAC 
ACCACTAAAG GAGATGGAGC GCGGAGCAAA GCCAAAGTGG TGACCACAAC AGGAGCAGTT 
CCAGGAAAGC CCACTATGAT CATCAGCACC ACCATAGGCA ACACGGCTCT GATCCAGTGG 
CAGCCGCCTA AAGAGATGGT GGGAGAGCTG ATGGGCTACA GACTCCGCTA TAAGCGTGAA 
GAAGAGGATA TTTACACTGT CCGAGACTTC AGACGCACTG ATGACCACTT CACTGTCACC 
GGCCTGCACA AGGGCGCGAC GTACATCTTC AAACTCTGCG CTAAAAACCG CGCCGGGAAT 
GGAGAGGAGT ACAGCAAAGA GATCAGCACC CCGGAGGATG TGCCCAGCAG TTACCCACAA 
AACCTCAAAG TCGTTGGATT GACCACAACC ACCACAAAAC TAGCCTGGGA TCCTCCGCCG 
CTGCCCGAGA GGAATGGGAA GATCGTACGA TATGTGGTGG TTTATCGAGA CATTAACAGC 
CATCAGAATC AGACCAACGG CACAGCGGAC ACGGAGATGA CCATTCAGGG CCTGAAGCCG 
GACACCACCT ATGATATCAG GGTGAGGGCG TTTACAGGGA AAGGTGGAGG TCCCATCAGC 
CCCAGCATCC AGAGCAGAAC CATGTCTACC TCCATGCCTG AGTTCACGAA AAACTTTGGT 
GTGAAAGCCG TGATGAAGAC CTCTGTCCTG CTCACATGGG AGGTGCCAGA AACATACAAG 
TCTCAAGTGC CTTTCAAGAT CCTGTATAAC CAGCAGAGTG TGGAGGTCCA GGGTGACCTG 
AAGAGGAAGC TGATTACGCG TCTGCAGCCG GACACTGATT ACTCCTTTGT CCTGATGAGT 
CGGGGGAACA GCGCTGGCGG ACTGCAGCAG CAGGTCTCCA TCCGCACCGC TCCAGACCTG 
TTCACTACCA AACCAGCACA CCAGAAACTA GACCCAGAGG AGGGCGGCAA ACTCGCCATC 
ACACTGCCCG AAGTGCACAA CAGCGCCCTC GTCAGGTGGT TTTATATCGT GGTTGTACCA 
GTAACTCCTT CAACTCTCCG CCATTGGGAA AACCCAGAAG ATATTGATCT GGATGAGCTG 
ATGGGGAATA ATAGAGACGC AGCTGTGAGG AGGAGGAGAA GACAAACTCA GGACTTTCTG 
CGGCCTTACA TTGCTGCCAA GCTGGAGTCT CTTCCAGAAA GATTTGTGTT GGGAGATGAG 
AAAACCTACA GCAACTACTA CAACAAACCT CTGCCGGGAC AGCAGCAGTA TCGCACATTT 
ATACTGGCGG CACTGAAAGA CAGAGACTCG ACGTTCACAG TGAGTCCGTT CTCTGAGCCC 
ATGATGGTGA AGCTCCACAG CGGGATGTCC CGTCACGTGG AGGACCCGGA GATGCTGTGG 
GTGATGGGAC CAGTGTTAGC CGTGGTCCTC ATCATCATCA TCGTCATCGC CATCCTCCTC 
TTCAAGAGGA AGCGCACGTC TCCCTCAGCT AAAGATGAGC ACTCAGCTGG AGTTAAAGAT 
CACCTGCTGG CTCATTCGTC AGATCCAGTG GAGATGAGGA GGCTCAACTA CCAGACACAA 
GGTTCCAGTG CCTTCAACTG TCCCAGCACT TCAAGAATGC GGGAACACCC TCCAGTGTCT 
GTGTGTGCTT TGGCTGACCA CATCGAGCGG CTGAGAGCCA ACGACAGCCT CCGCTTCTCG 
CAGGAGTATG AGTCTGTTGA CCCTGGCCAG CAGTTCACCT GGGAAAACTC CAACTTAGAG 
GTCAACAAGC CCAAGAACCG CTACGCAAAC GTCATCGCTT ACGATCACTC CAGGGTTGTG 
CTGACTCCTG TGGATGGTGT TCCCGGAAGT GACTACATCA ATGGAAACTA CATTGATGGC 
TACCGTAAGC AGAACGCCTA CATCGCCACG CAGGGTCCGC TGCCTGAGAC GCTCAGTGAC 
TTCTGGAGGA TGGTGTGGGA ACAGAGAACC AGCACTATCG TCATGATGAC CCGACTGGAG 
GAGAAGAGCA GGGTGAAGTG TGATCAGTAC TGGCCAAGCC GAGGAACAGA GACTTACGGG 
ATGATTCAAG TGTCCATGCT GGATACGGTG GAGCTGGCCA CATACAGCGT CAGAACCTTC 
GCTCTCTACA AGAATGGATC CAGCGAGAAA CGGGAGGTTC GGCAGTTCCA GTTCATGGCG 
TGGCCGGATC ACGGTGTTCC CGAATATCCC ACACCCATTT TAGCCTTCTT ACGCAGGGTC 
AAAGCCTGCA ACCCACCTGA CGCGGGACCC ATGGTGGTGC ACTGCAGCGC TGGCGTGGGT 
CGGACGGGCT GTTTCATCGT AATAGACGCC ATGCTGGAGC GGATGAAGCA CGAGAAGTCG 
GTGGACATCT ACGGTCACGT GACCTGCATG CGTTCTCAGA GGAACTACAT GGTGCAGACA 
GAGGATCAGT ACATTTTCAT TCACGAGGCC CTGCTGGAGG CTGCCACCTG CGGGAACACA 
GAGGTGCCGG CGCGGAGCCT CTACACACAC ATCCAGAAAC TCACACAGGC GCCGCCTGGA 
GACACCGTCA CCGCCATGGA GCTCGAATTT AAGAAACTGG CCAACTCTAA AGCACACACC 
TCCAGATTCA TCAGCGCTAG TCTGCCCTGC AACAAATTCA AGAACCGGCT GGTGAACATC 
ATGCCTTTCG AGTCCAGCCG TGTTTGTCTG CAGCCAATCA GAGGCGTGGA GGGCTCCGAC 
TACATCAACG CCAGCTTCAT CGACGGATAC AGGCAGCAGA AGGCATATAT CGCCACTCAA 
GGCCCGCTGT CTGAAACCAC AGAGGACTTC TGGAGGATGC TTTGGGAGCA CAACTCCACC 
ATCGTGGTGA TGCTGACGAA GCTTAGAGAG ATGGGACGGG AGAAATGTCA TCAGTACTGG 
CCTGCAGAGA GATCTGCGCG CTACCAGTAT TTCGTTGTGG ATCCTATGGC TGAATATAAC 
ATGCCACAGT ACATCTTACG GGAGTTTAAA GTCACAGACG CCAGAGATGG CCAGTCTCGG 
ACTATCCGAC AGTTTCAGTT CACAGATTGG CCGGAACAAG GTGTGCCAAA GACAGGAGAG 
GGCTTTATTG ATTTTATTGG TCAAGTCCAC AAAACCAAGG AGCAGTTTGG ACAGGACGGA 
CCAATTACTG TTCACTGCAG TGCCGGTGTG GGCAGAACAG GCGTCTTCAT CACTTTAAGT 
ATTGTTCTGG AGCGGATGAG GTATGAAGGA GTGGTGGACA TGTTCCAGAC CATAAAAACA 
CTCCGCACGC AGAGACCTGC CATGGTACAG ACCGAGGACC AGTACCAGCT GTGCTACAGA 
GCAGCTCTGG AGTACCTGGG CAGCTTCGAT CACTATGCAA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332438.1
CDS	1459..7203
Translation	MVPQTRQGSARHGATSLLLLLCLAVPIHADSMPSFVKTPEDQTGISGGVASFVCQAVGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEEDQIPHGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEISWFKDFLPVDINSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAVNSAGTRYSAPANLYVRDQREVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMKGEQELTKEEEMPIGRNVLELTNIRQSGNYTCVAISSLGMIDTTAQITVKALPRPPASLIVTETTATSVTLTWDSGNPEPVSYYVIQYRSKISDNGFQEVDGVASTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSGMVETRTSEQAPSSPPLRVQARMLSATTMLVQWEPPEEPNGQIRGYRVYYTSDLHFPLSTWQKHNTEDSSLTTISGLVPDITYGLRVLAFTSVGDGPPSDILQVKTQQGVPAQPTGFEAEAELDTRIMLSWLWPVQDQITKYELTYWEADSDNKHHVIFDPAGSYAIDSLKPDTLYKFSLAARSEMGLGVYTQPIEARTAQSAPSAPPQEVHLISLSSSSIKVSWVAPPAASRHGSIVRYSLAYQAVSGEDQERHEVKDIPADVSSYVLEGLEKWTEYTVWVKAHTDVGPGPESGSTRIRTQEDVPGAPPRRVEVDNINSTALRVSWKPPLQQKQHGHIRGYQVVYSRLENGEPRGQPIILDVALPEAQEAVISGLLPETTYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTIGNTALIQWQPPKEMVGELMGYRLRYKREEEDIYTVRDFRRTDDHFTVTGLHKGATYIFKLCAKNRAGNGEEYSKEISTPEDVPSSYPQNLKVVGLTTTTTKLAWDPPPLPERNGKIVRYVVVYRDINSHQNQTNGTADTEMTIQGLKPDTTYDIRVRAFTGKGGGPISPSIQSRTMSTSMPEFTKNFGVKAVMKTSVLLTWEVPETYKSQVPFKILYNQQSVEVQGDLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFTTKPAHQKLDPEEGGKLAITLPEVHNSALVRWFYIVVVPVTPSTLRHWENPEDIDLDELMGNNRDAAVRRRRRQTQDFLRPYIAAKLESLPERFVLGDEKTYSNYYNKPLPGQQQYRTFILAALKDRDSTFTVSPFSEPMMVKLHSGMSRHVEDPEMLWVMGPVLAVVLIIIIVIAILLFKRKRTSPSAKDEHSAGVKDHLLAHSSDPVEMRRLNYQTQGSSAFNCPSTSRMREHPPVSVCALADHIERLRANDSLRFSQEYESVDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINGNYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRTSTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVSMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARSLYTHIQKLTQAPPGDTVTAMELEFKKLANSKAHTSRFISASLPCNKFKNRLVNIMPFESSRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLSETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTIKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021476763.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476763.1

ORF Insert Sequence:

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGACC AGCGGGAAGT CCGACGGGTT 
CCCCCACGTT TTTCAATCCC TCCAACCAAT CACGAGGTGA TGCCGGGTGG CAGTGTGAAC 
CTCACCTGCG TGGCAGTGGG CGCTCCAATG CCCTATGTGA AGTGGATGAA GGGCGAACAG 
GAGCTGACCA AAGAAGAGGA GATGCCCATC GGCCGAAATG TGCTGGAGCT CACCAACATC 
CGCCAGTCGG GGAACTACAC CTGCGTGGCC ATATCATCAC TGGGCATGAT CGACACCACA 
GCGCAGATCA CTGTTAAAGC TCTTCCTCGT CCTCCAGCCT CTCTGATTGT CACTGAAACC 
ACCGCCACCA GTGTGACTTT AACATGGGAT TCTGGCAACC CAGAACCCGT ATCATATTAC 
GTGATCCAGT ATCGATCCAA AATCTCTGAC AATGGTTTCC AGGAGGTGGA CGGCGTGGCA 
TCCACCCGCT ACAGCATCGG CGGGTTGAGT CCGTACTCAG AGTATGAGTT TCGAGTAATG 
GCGGTCAATA ACATTGGTCG CGGGCCGCCC AGCGGGATGG TGGAGACTCG TACCAGTGAA 
CAGGCACCGT CATCTCCTCC GCTGCGCGTT CAAGCCCGGA TGTTGAGCGC TACGACTATG 
CTGGTGCAAT GGGAACCTCC TGAAGAGCCC AACGGACAGA TTCGAGGATA TCGAGTGTAC 
TACACGTCTG ACCTGCACTT CCCGCTCAGC ACATGGCAGA AGCACAACAC TGAGGACAGC 
AGTCTGACCA CCATCTCCGG CCTGGTTCCT GACATCACGT ATGGGCTCCG TGTGCTGGCC 
TTCACCTCTG TTGGAGATGG ACCACCTTCC GATATACTGC AGGTTAAAAC ACAGCAAGGA 
GTCCCTGCTC AGCCGACTGG TTTTGAAGCT GAAGCCGAGT TGGACACGCG GATCATGCTG 
TCATGGTTGT GGCCCGTCCA AGATCAGATC ACCAAATATG AGCTCACATA CTGGGAGGCT 
GACTCTGACA ACAAGCATCA TGTCATCTTT GACCCGGCCG GTTCATATGC TATTGACAGC 
CTAAAGCCAG ACACTCTCTA TAAATTCTCT CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG 
GTTTACACAC AGCCTATCGA GGCCCGCACA GCCCAGTCCG CCCCGTCCGC TCCCCCACAA 
GAGGTGCACC TGATCAGCCT GAGCTCCAGC AGCATCAAGG TGAGTTGGGT GGCCCCTCCG 
GCCGCCAGCC GGCACGGCAG CATCGTCCGC TACTCTCTGG CCTATCAGGC AGTGTCTGGC 
GAGGACCAGG AGCGCCACGA GGTCAAAGAC ATCCCTGCTG ATGTGTCCAG CTATGTTCTG 
GAGGGTCTGG AGAAATGGAC TGAGTATACG GTGTGGGTCA AAGCGCACAC AGATGTGGGT 
CCCGGGCCAG AAAGTGGCAG CACCCGCATC AGAACCCAAG AGGATGTGCC TGGTGCTCCT 
CCACGCAGGG TTGAGGTGGA CAACATCAAC TCCACTGCAC TGCGTGTGTC CTGGAAGCCG 
CCTCTGCAGC AGAAACAACA CGGCCACATA CGTGGATATC AGGTGGTTTA CTCACGCCTG 
GAGAATGGAG AGCCGCGCGG ACAGCCCATC ATTCTGGACG TGGCGCTGCC AGAGGCACAG 
GAGGCCGTGA TCAGTGGTCT TCTCCCTGAA ACCACATATT CAGTGACTGT AGCCGCGTAC 
ACCACTAAAG GAGATGGAGC GCGGAGCAAA GCCAAAGTGG TGACCACAAC AGGAGCAGTT 
CCAGGAAAGC CCACTATGAT CATCAGCACC ACCATAGGCA ACACGGCTCT GATCCAGTGG 
CAGCCGCCTA AAGAGATGGT GGGAGAGCTG ATGGGCTACA GACTCCGCTA TAAGCGTGAA 
GAAGAGGATA TTTACACTGT CCGAGACTTC AGACGCACTG ATGACCACTT CACTGTCACC 
GGCCTGCACA AGGGCGCGAC GTACATCTTC AAACTCTGCG CTAAAAACCG CGCCGGGAAT 
GGAGAGGAGT ACAGCAAAGA GATCAGCACC CCGGAGGATG TGCCCAGCAG TTACCCACAA 
AACCTCAAAG TCGTTGGATT GACCACAACC ACCACAAAAC TAGCCTGGGA TCCTCCGCCG 
CTGCCCGAGA GGAATGGGAA GATCGTACGA TATGTGGTGG TTTATCGAGA CATTAACAGC 
CATCAGAATC AGACCAACGG CACAGCGGAC ACGGAGATGA CCATTCAGGG CCTGAAGCCG 
GACACCACCT ATGATATCAG GGTGAGGGCG TTTACAGGGA AAGGTGGAGG TCCCATCAGC 
CCCAGCATCC AGAGCAGAAC CATGTCTACC TCCATGCCTG AGTTCACGAA AAACTTTGGT 
GTGAAAGCCG TGATGAAGAC CTCTGTCCTG CTCACATGGG AGGTGCCAGA AACATACAAG 
TCTCAAGTGC CTTTCAAGAT CCTGTATAAC CAGCAGAGTG TGGAGGTCCA GGGTGACCTG 
AAGAGGAAGC TGATTACGCG TCTGCAGCCG GACACTGATT ACTCCTTTGT CCTGATGAGT 
CGGGGGAACA GCGCTGGCGG ACTGCAGCAG CAGGTCTCCA TCCGCACCGC TCCAGACCTG 
TTCACTACCA AACCAGCACA CCAGAAACTA GACCCAGAGG AGGGCGGCAA ACTCGCCATC 
ACACTGCCCG AAGTGCACAA CAGCGCCCTC GTCAGGTGGT TTTATATCGT GGTTGTACCA 
GTAACTCCTT CAACTCTCCG CCATTGGGAA AACCCAGAAG ATATTGATCT GGATGAGCTG 
ATGGGGAATA ATAGAGACGC AGCTGTGAGG AGGAGGAGAA GACAAACTCA GGACTTTCTG 
CGGCCTTACA TTGCTGCCAA GCTGGAGTCT CTTCCAGAAA GATTTGTGTT GGGAGATGAG 
AAAACCTACA GCAACTACTA CAACAAACCT CTGCCGGGAC AGCAGCAGTA TCGCACATTT 
ATACTGGCGG CACTGAAAGA CAGAGACTCG ACGTTCACAG TGAGTCCGTT CTCTGAGCCC 
ATGATGGTGA AGCTCCACAG CGGGATGTCC CGTCACGTGG AGGACCCGGA GATGCTGTGG 
GTGATGGGAC CAGTGTTAGC CGTGGTCCTC ATCATCATCA TCGTCATCGC CATCCTCCTC 
TTCAAGAGGA AGCGCACGTC TCCCTCAGCT AAAGATGAGC ACTCAGCTGG AGTTAAAGAT 
CACCTGCTGG CTCATTCGTC AGATCCAGTG GAGATGAGGA GGCTCAACTA CCAGACACAA 
GGTTCCAGTG CCTTCAACTG TCCCAGCACT TCAAGAATGC GGGAACACCC TCCAGTGTCT 
GTGTGTGCTT TGGCTGACCA CATCGAGCGG CTGAGAGCCA ACGACAGCCT CCGCTTCTCG 
CAGGAGTATG AGTCTGTTGA CCCTGGCCAG CAGTTCACCT GGGAAAACTC CAACTTAGAG 
GTCAACAAGC CCAAGAACCG CTACGCAAAC GTCATCGCTT ACGATCACTC CAGGGTTGTG 
CTGACTCCTG TGGATGGTGT TCCCGGAAGT GACTACATCA ATGGAAACTA CATTGATGGC 
TACCGTAAGC AGAACGCCTA CATCGCCACG CAGGGTCCGC TGCCTGAGAC GCTCAGTGAC 
TTCTGGAGGA TGGTGTGGGA ACAGAGAACC AGCACTATCG TCATGATGAC CCGACTGGAG 
GAGAAGAGCA GGGTGAAGTG TGATCAGTAC TGGCCAAGCC GAGGAACAGA GACTTACGGG 
ATGATTCAAG TGTCCATGCT GGATACGGTG GAGCTGGCCA CATACAGCGT CAGAACCTTC 
GCTCTCTACA AGAATGGATC CAGCGAGAAA CGGGAGGTTC GGCAGTTCCA GTTCATGGCG 
TGGCCGGATC ACGGTGTTCC CGAATATCCC ACACCCATTT TAGCCTTCTT ACGCAGGGTC 
AAAGCCTGCA ACCCACCTGA CGCGGGACCC ATGGTGGTGC ACTGCAGCGC TGGCGTGGGT 
CGGACGGGCT GTTTCATCGT AATAGACGCC ATGCTGGAGC GGATGAAGCA CGAGAAGTCG 
GTGGACATCT ACGGTCACGT GACCTGCATG CGTTCTCAGA GGAACTACAT GGTGCAGACA 
GAGGATCAGT ACATTTTCAT TCACGAGGCC CTGCTGGAGG CTGCCACCTG CGGGAACACA 
GAGGTGCCGG CGCGGAGCCT CTACACACAC ATCCAGAAAC TCACACAGGC GCCGCCTGGA 
GACACCGTCA CCGCCATGGA GCTCGAATTT AAGAAACTGG CCAACTCTAA AGCACACACC 
TCCAGATTCA TCAGCGCTAG TCTGCCCTGC AACAAATTCA AGAACCGGCT GGTGAACATC 
ATGCCTTTCG AGTCCAGCCG TGTTTGTCTG CAGCCAATCA GAGGCGTGGA GGGCTCCGAC 
TACATCAACG CCAGCTTCAT CGACGGATAC AGGCAGCAGA AGGCATATAT CGCCACTCAA 
GGCCCGCTGT CTGAAACCAC AGAGGACTTC TGGAGGATGC TTTGGGAGCA CAACTCCACC 
ATCGTGGTGA TGCTGACGAA GCTTAGAGAG ATGGGACGGG AGAAATGTCA TCAGTACTGG 
CCTGCAGAGA GATCTGCGCG CTACCAGTAT TTCGTTGTGG ATCCTATGGC TGAATATAAC 
ATGCCACAGT ACATCTTACG GGAGTTTAAA GTCACAGACG CCAGAGATGG CCAGTCTCGG 
ACTATCCGAC AGTTTCAGTT CACAGATTGG CCGGAACAAG GTGTGCCAAA GACAGGAGAG 
GGCTTTATTG ATTTTATTGG TCAAGTCCAC AAAACCAAGG AGCAGTTTGG ACAGGACGGA 
CCAATTACTG TTCACTGCAG TGCCGGTGTG GGCAGAACAG GCGTCTTCAT CACTTTAAGT 
ATTGTTCTGG AGCGGATGAG GTATGAAGGA GTGGTGGACA TGTTCCAGAC CATAAAAACA 
CTCCGCACGC AGAGACCTGC CATGGTACAG ACCGAGGACC AGTACCAGCT GTGCTACAGA 
GCAGCTCTGG AGTACCTGGG CAGCTTCGAT CACTATGCAA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfa ( XM_021476764.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332439.1 Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X5, mRNA.

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CloneID	ODa142905
Clone ID Related Accession (Same CDS sequence)	XM_021476764.1
Accession Version	XM_021476764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGA GCGCTGCAGA TCGAGAACAG CGAGGAATCA 
GACCAGGGCA AGTATGAATG TGTGGCGGTG AACAGCGCGG GAACTCGATA CTCGGCTCCT 
GCAAACCTCT ACGTCAGAGA CCAGCGGGAA GTCCGACGGG TTCCCCCACG TTTTTCAATC 
CCTCCAACCA ATCACGAGGT GATGCCGGGT GGCAGTGTGA ACCTCACCTG CGTGGCAGTG 
GGCGCTCCAA TGCCCTATGT GAAGTGGATG AAGGGCGAAC AGGAGCTGAC CAAAGAAGAG 
GAGATGCCCA TCGGCCGAAA TGTGCTGGAG CTCACCAACA TCCGCCAGTC GGGGAACTAC 
ACCTGCGTGG CCATATCATC ACTGGGCATG ATCGACACCA CAGCGCAGAT CACTGTTAAA 
GCTCTTCCTC GTCCTCCAGC CTCTCTGATT GTCACTGAAA CCACCGCCAC CAGTGTGACT 
TTAACATGGG ATTCTGGCAA CCCAGAACCC GTATCATATT ACGTGATCCA GTATCGATCC 
AAAATCTCTG ACAATGGTTT CCAGGAGGTG GACGGCGTGG CATCCACCCG CTACAGCATC 
GGCGGGTTGA GTCCGTACTC AGAGTATGAG TTTCGAGTAA TGGCGGTCAA TAACATTGGT 
CGCGGGCCGC CCAGCGGGAT GGTGGAGACT CGTACCAGTG AACAGGCACC GTCATCTCCT 
CCGCTGCGCG TTCAAGCCCG GATGTTGAGC GCTACGACTA TGCTGGTGCA ATGGGAACCT 
CCTGAAGAGC CCAACGGACA GATTCGAGGA TATCGAGTGT ACTACACGTC TGACCTGCAC 
TTCCCGCTCA GCACATGGCA GAAGCACAAC ACTGAGGACA GCAGTCTGAC CACCATCTCC 
GGCCTGGTTC CTGACATCAC GTATGGGCTC CGTGTGCTGG CCTTCACCTC TGTTGGAGAT 
GGACCACCTT CCGATATACT GCAGGTTAAA ACACAGCAAG GAGTCCCTGC TCAGCCGACT 
GGTTTTGAAG CTGAAGCCGA GTTGGACACG CGGATCATGC TGTCATGGTT GTGGCCCGTC 
CAAGATCAGA TCACCAAATA TGAGCTCACA TACTGGGAGG CTGACTCTGA CAACAAGCAT 
CATGTCATCT TTGACCCGGC CGGTTCATAT GCTATTGACA GCCTAAAGCC AGACACTCTC 
TATAAATTCT CTCTGGCGGC GCGCTCTGAG ATGGGGCTCG GGGTTTACAC ACAGCCTATC 
GAGGCCCGCA CAGCCCAGTC CGCCCCGTCC GCTCCCCCAC AAGAGGTGCA CCTGATCAGC 
CTGAGCTCCA GCAGCATCAA GGTGAGTTGG GTGGCCCCTC CGGCCGCCAG CCGGCACGGC 
AGCATCGTCC GCTACTCTCT GGCCTATCAG GCAGTGTCTG GCGAGGACCA GGAGCGCCAC 
GAGGTCAAAG ACATCCCTGC TGATGTGTCC AGCTATGTTC TGGAGGGTCT GGAGAAATGG 
ACTGAGTATA CGGTGTGGGT CAAAGCGCAC ACAGATGTGG GTCCCGGGCC AGAAAGTGGC 
AGCACCCGCA TCAGAACCCA AGAGGATGTG CCTGGTGCTC CTCCACGCAG GGTTGAGGTG 
GACAACATCA ACTCCACTGC ACTGCGTGTG TCCTGGAAGC CGCCTCTGCA GCAGAAACAA 
CACGGCCACA TACGTGGATA TCAGGTGGTT TACTCACGCC TGGAGAATGG AGAGCCGCGC 
GGACAGCCCA TCATTCTGGA CGTGGCGCTG CCAGAGGCAC AGGAGGCCGT GATCAGTGGT 
CTTCTCCCTG AAACCACATA TTCAGTGACT GTAGCCGCGT ACACCACTAA AGGAGATGGA 
GCGCGGAGCA AAGCCAAAGT GGTGACCACA ACAGGAGCAG TTCCAGGAAA GCCCACTATG 
ATCATCAGCA CCACCATAGG CAACACGGCT CTGATCCAGT GGCAGCCGCC TAAAGAGATG 
GTGGGAGAGC TGATGGGCTA CAGACTCCGC TATAAGCGTG AAGAAGAGGA TATTTACACT 
GTCCGAGACT TCAGACGCAC TGATGACCAC TTCACTGTCA CCGGCCTGCA CAAGGGCGCG 
ACGTACATCT TCAAACTCTG CGCTAAAAAC CGCGCCGGGA ATGGAGAGGA GTACAGCAAA 
GAGATCAGCA CCCCGGAGGA TGTGCCCAGC AGTTACCCAC AAAACCTCAA AGTCGTTGGA 
TTGACCACAA CCACCACAAA ACTAGCCTGG GATCCTCCGC CGCTGCCCGA GAGGAATGGG 
AAGATCGTAC GATATGTGGT GGTTTATCGA GACATTAACA GCCATCAGAA TCAGACCAAC 
GGCACAGCGG ACACGGAGAT GACCATTCAG GGCCTGAAGC CGGACACCAC CTATGATATC 
AGGGTGAGGG CGTTTACAGG GAAAGGTGGA GGTCCCATCA GCCCCAGCAT CCAGAGCAGA 
ACCATGTCTA CCTCCATGCC TGAGTTCACG AAAAACTTTG GTGTGAAAGC CGTGATGAAG 
ACCTCTGTCC TGCTCACATG GGAGGTGCCA GAAACATACA AGTCTCAAGT GCCTTTCAAG 
ATCCTGTATA ACCAGCAGAG TGTGGAGGTC CAGGGTGACC TGAAGAGGAA GCTGATTACG 
CGTCTGCAGC CGGACACTGA TTACTCCTTT GTCCTGATGA GTCGGGGGAA CAGCGCTGGC 
GGACTGCAGC AGCAGGTCTC CATCCGCACC GCTCCAGACC TGTTCACTAC CAAACCAGCA 
CACCAGAAAC TAGACCCAGA GGAGGGCGGC AAACTCGCCA TCACACTGCC CGAAGTGCAC 
AACAGCGCCC TCGTCAGGTG GTTTTATATC GTGGTTGTAC CAGTAACTCC TTCAACTCTC 
CGCCATTGGG AAAACCCAGA AGATATTGAT CTGGATGAGC TGATGGGGAA TAATAGAGAC 
GCAGCTGTGA GGAGGAGGAG AAGACAAACT CAGGACTTTC TGCGGCCTTA CATTGCTGCC 
AAGCTGGAGT CTCTTCCAGA AAGATTTGTG TTGGGAGATG AGAAAACCTA CAGCAACTAC 
TACAACAAAC CTCTGCCGGG ACAGCAGCAG TATCGCACAT TTATACTGGC GGCACTGAAA 
GACAGAGACT CGACGTTCAC AGTGAGTCCG TTCTCTGAGC CCATGATGGT GAAGCTCCAC 
AGCGGGATGT CCCGTCACGT GGAGGACCCG GAGATGCTGT GGGTGATGGG ACCAGTGTTA 
GCCGTGGTCC TCATCATCAT CATCGTCATC GCCATCCTCC TCTTCAAGAG CAAACAGGAG 
AGGAAGCGCA CGTCTCCCTC AGCTAAAGAT GAGCACTCAG CTGGAGTTAA AGATCACCTG 
CTGGCTCATT CGTCAGATCC AGTGGAGATG AGGAGGCTCA ACTACCAGAC ACAAGGTTCC 
AGTGCCTTCA ACTGTCCCAG CACTTCAAGA ATGCGGGAAC ACCCTCCAGT GTCTGTGTGT 
GCTTTGGCTG ACCACATCGA GCGGCTGAGA GCCAACGACA GCCTCCGCTT CTCGCAGGAG 
TATGAGTCTG TTGACCCTGG CCAGCAGTTC ACCTGGGAAA ACTCCAACTT AGAGGTCAAC 
AAGCCCAAGA ACCGCTACGC AAACGTCATC GCTTACGATC ACTCCAGGGT TGTGCTGACT 
CCTGTGGATG GTGTTCCCGG AAGTGACTAC ATCAATGGAA ACTACATTGA TGGCTACCGT 
AAGCAGAACG CCTACATCGC CACGCAGGGT CCGCTGCCTG AGACGCTCAG TGACTTCTGG 
AGGATGGTGT GGGAACAGAG AACCAGCACT ATCGTCATGA TGACCCGACT GGAGGAGAAG 
AGCAGGGTGA AGTGTGATCA GTACTGGCCA AGCCGAGGAA CAGAGACTTA CGGGATGATT 
CAAGTGTCCA TGCTGGATAC GGTGGAGCTG GCCACATACA GCGTCAGAAC CTTCGCTCTC 
TACAAGAATG GATCCAGCGA GAAACGGGAG GTTCGGCAGT TCCAGTTCAT GGCGTGGCCG 
GATCACGGTG TTCCCGAATA TCCCACACCC ATTTTAGCCT TCTTACGCAG GGTCAAAGCC 
TGCAACCCAC CTGACGCGGG ACCCATGGTG GTGCACTGCA GCGCTGGCGT GGGTCGGACG 
GGCTGTTTCA TCGTAATAGA CGCCATGCTG GAGCGGATGA AGCACGAGAA GTCGGTGGAC 
ATCTACGGTC ACGTGACCTG CATGCGTTCT CAGAGGAACT ACATGGTGCA GACAGAGGAT 
CAGTACATTT TCATTCACGA GGCCCTGCTG GAGGCTGCCA CCTGCGGGAA CACAGAGGTG 
CCGGCGCGGA GCCTCTACAC ACACATCCAG AAACTCACAC AGGCGCCGCC TGGAGACACC 
GTCACCGCCA TGGAGCTCGA ATTTAAGAAA CTGGCCAACT CTAAAGCACA CACCTCCAGA 
TTCATCAGCG CTAGTCTGCC CTGCAACAAA TTCAAGAACC GGCTGGTGAA CATCATGCCT 
TTCGAGTCCA GCCGTGTTTG TCTGCAGCCA ATCAGAGGCG TGGAGGGCTC CGACTACATC 
AACGCCAGCT TCATCGACGG ATACAGGCAG CAGAAGGCAT ATATCGCCAC TCAAGGCCCG 
CTGTCTGAAA CCACAGAGGA CTTCTGGAGG ATGCTTTGGG AGCACAACTC CACCATCGTG 
GTGATGCTGA CGAAGCTTAG AGAGATGGGA CGGGAGAAAT GTCATCAGTA CTGGCCTGCA 
GAGAGATCTG CGCGCTACCA GTATTTCGTT GTGGATCCTA TGGCTGAATA TAACATGCCA 
CAGTACATCT TACGGGAGTT TAAAGTCACA GACGCCAGAG ATGGCCAGTC TCGGACTATC 
CGACAGTTTC AGTTCACAGA TTGGCCGGAA CAAGGTGTGC CAAAGACAGG AGAGGGCTTT 
ATTGATTTTA TTGGTCAAGT CCACAAAACC AAGGAGCAGT TTGGACAGGA CGGACCAATT 
ACTGTTCACT GCAGTGCCGG TGTGGGCAGA ACAGGCGTCT TCATCACTTT AAGTATTGTT 
CTGGAGCGGA TGAGGTATGA AGGAGTGGTG GACATGTTCC AGACCATAAA AACACTCCGC 
ACGCAGAGAC CTGCCATGGT ACAGACCGAG GACCAGTACC AGCTGTGCTA CAGAGCAGCT 
CTGGAGTACC TGGGCAGCTT CGATCACTAT GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332439.1
CDS	1458..7196
Translation	MVPQTRQGSARHGATSLLLLLCLAVPIHADSMPSFVKTPEDQTGISGGVASFVCQAVGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEEDQIPHGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEISWFKDFLPVDINSSNGRIKQLRSGALQIENSEESDQGKYECVAVNSAGTRYSAPANLYVRDQREVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMKGEQELTKEEEMPIGRNVLELTNIRQSGNYTCVAISSLGMIDTTAQITVKALPRPPASLIVTETTATSVTLTWDSGNPEPVSYYVIQYRSKISDNGFQEVDGVASTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSGMVETRTSEQAPSSPPLRVQARMLSATTMLVQWEPPEEPNGQIRGYRVYYTSDLHFPLSTWQKHNTEDSSLTTISGLVPDITYGLRVLAFTSVGDGPPSDILQVKTQQGVPAQPTGFEAEAELDTRIMLSWLWPVQDQITKYELTYWEADSDNKHHVIFDPAGSYAIDSLKPDTLYKFSLAARSEMGLGVYTQPIEARTAQSAPSAPPQEVHLISLSSSSIKVSWVAPPAASRHGSIVRYSLAYQAVSGEDQERHEVKDIPADVSSYVLEGLEKWTEYTVWVKAHTDVGPGPESGSTRIRTQEDVPGAPPRRVEVDNINSTALRVSWKPPLQQKQHGHIRGYQVVYSRLENGEPRGQPIILDVALPEAQEAVISGLLPETTYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTIGNTALIQWQPPKEMVGELMGYRLRYKREEEDIYTVRDFRRTDDHFTVTGLHKGATYIFKLCAKNRAGNGEEYSKEISTPEDVPSSYPQNLKVVGLTTTTTKLAWDPPPLPERNGKIVRYVVVYRDINSHQNQTNGTADTEMTIQGLKPDTTYDIRVRAFTGKGGGPISPSIQSRTMSTSMPEFTKNFGVKAVMKTSVLLTWEVPETYKSQVPFKILYNQQSVEVQGDLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFTTKPAHQKLDPEEGGKLAITLPEVHNSALVRWFYIVVVPVTPSTLRHWENPEDIDLDELMGNNRDAAVRRRRRQTQDFLRPYIAAKLESLPERFVLGDEKTYSNYYNKPLPGQQQYRTFILAALKDRDSTFTVSPFSEPMMVKLHSGMSRHVEDPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRTSPSAKDEHSAGVKDHLLAHSSDPVEMRRLNYQTQGSSAFNCPSTSRMREHPPVSVCALADHIERLRANDSLRFSQEYESVDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINGNYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRTSTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVSMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARSLYTHIQKLTQAPPGDTVTAMELEFKKLANSKAHTSRFISASLPCNKFKNRLVNIMPFESSRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLSETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTIKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021476764.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476764.1

ORF Insert Sequence:

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGA GCGCTGCAGA TCGAGAACAG CGAGGAATCA 
GACCAGGGCA AGTATGAATG TGTGGCGGTG AACAGCGCGG GAACTCGATA CTCGGCTCCT 
GCAAACCTCT ACGTCAGAGA CCAGCGGGAA GTCCGACGGG TTCCCCCACG TTTTTCAATC 
CCTCCAACCA ATCACGAGGT GATGCCGGGT GGCAGTGTGA ACCTCACCTG CGTGGCAGTG 
GGCGCTCCAA TGCCCTATGT GAAGTGGATG AAGGGCGAAC AGGAGCTGAC CAAAGAAGAG 
GAGATGCCCA TCGGCCGAAA TGTGCTGGAG CTCACCAACA TCCGCCAGTC GGGGAACTAC 
ACCTGCGTGG CCATATCATC ACTGGGCATG ATCGACACCA CAGCGCAGAT CACTGTTAAA 
GCTCTTCCTC GTCCTCCAGC CTCTCTGATT GTCACTGAAA CCACCGCCAC CAGTGTGACT 
TTAACATGGG ATTCTGGCAA CCCAGAACCC GTATCATATT ACGTGATCCA GTATCGATCC 
AAAATCTCTG ACAATGGTTT CCAGGAGGTG GACGGCGTGG CATCCACCCG CTACAGCATC 
GGCGGGTTGA GTCCGTACTC AGAGTATGAG TTTCGAGTAA TGGCGGTCAA TAACATTGGT 
CGCGGGCCGC CCAGCGGGAT GGTGGAGACT CGTACCAGTG AACAGGCACC GTCATCTCCT 
CCGCTGCGCG TTCAAGCCCG GATGTTGAGC GCTACGACTA TGCTGGTGCA ATGGGAACCT 
CCTGAAGAGC CCAACGGACA GATTCGAGGA TATCGAGTGT ACTACACGTC TGACCTGCAC 
TTCCCGCTCA GCACATGGCA GAAGCACAAC ACTGAGGACA GCAGTCTGAC CACCATCTCC 
GGCCTGGTTC CTGACATCAC GTATGGGCTC CGTGTGCTGG CCTTCACCTC TGTTGGAGAT 
GGACCACCTT CCGATATACT GCAGGTTAAA ACACAGCAAG GAGTCCCTGC TCAGCCGACT 
GGTTTTGAAG CTGAAGCCGA GTTGGACACG CGGATCATGC TGTCATGGTT GTGGCCCGTC 
CAAGATCAGA TCACCAAATA TGAGCTCACA TACTGGGAGG CTGACTCTGA CAACAAGCAT 
CATGTCATCT TTGACCCGGC CGGTTCATAT GCTATTGACA GCCTAAAGCC AGACACTCTC 
TATAAATTCT CTCTGGCGGC GCGCTCTGAG ATGGGGCTCG GGGTTTACAC ACAGCCTATC 
GAGGCCCGCA CAGCCCAGTC CGCCCCGTCC GCTCCCCCAC AAGAGGTGCA CCTGATCAGC 
CTGAGCTCCA GCAGCATCAA GGTGAGTTGG GTGGCCCCTC CGGCCGCCAG CCGGCACGGC 
AGCATCGTCC GCTACTCTCT GGCCTATCAG GCAGTGTCTG GCGAGGACCA GGAGCGCCAC 
GAGGTCAAAG ACATCCCTGC TGATGTGTCC AGCTATGTTC TGGAGGGTCT GGAGAAATGG 
ACTGAGTATA CGGTGTGGGT CAAAGCGCAC ACAGATGTGG GTCCCGGGCC AGAAAGTGGC 
AGCACCCGCA TCAGAACCCA AGAGGATGTG CCTGGTGCTC CTCCACGCAG GGTTGAGGTG 
GACAACATCA ACTCCACTGC ACTGCGTGTG TCCTGGAAGC CGCCTCTGCA GCAGAAACAA 
CACGGCCACA TACGTGGATA TCAGGTGGTT TACTCACGCC TGGAGAATGG AGAGCCGCGC 
GGACAGCCCA TCATTCTGGA CGTGGCGCTG CCAGAGGCAC AGGAGGCCGT GATCAGTGGT 
CTTCTCCCTG AAACCACATA TTCAGTGACT GTAGCCGCGT ACACCACTAA AGGAGATGGA 
GCGCGGAGCA AAGCCAAAGT GGTGACCACA ACAGGAGCAG TTCCAGGAAA GCCCACTATG 
ATCATCAGCA CCACCATAGG CAACACGGCT CTGATCCAGT GGCAGCCGCC TAAAGAGATG 
GTGGGAGAGC TGATGGGCTA CAGACTCCGC TATAAGCGTG AAGAAGAGGA TATTTACACT 
GTCCGAGACT TCAGACGCAC TGATGACCAC TTCACTGTCA CCGGCCTGCA CAAGGGCGCG 
ACGTACATCT TCAAACTCTG CGCTAAAAAC CGCGCCGGGA ATGGAGAGGA GTACAGCAAA 
GAGATCAGCA CCCCGGAGGA TGTGCCCAGC AGTTACCCAC AAAACCTCAA AGTCGTTGGA 
TTGACCACAA CCACCACAAA ACTAGCCTGG GATCCTCCGC CGCTGCCCGA GAGGAATGGG 
AAGATCGTAC GATATGTGGT GGTTTATCGA GACATTAACA GCCATCAGAA TCAGACCAAC 
GGCACAGCGG ACACGGAGAT GACCATTCAG GGCCTGAAGC CGGACACCAC CTATGATATC 
AGGGTGAGGG CGTTTACAGG GAAAGGTGGA GGTCCCATCA GCCCCAGCAT CCAGAGCAGA 
ACCATGTCTA CCTCCATGCC TGAGTTCACG AAAAACTTTG GTGTGAAAGC CGTGATGAAG 
ACCTCTGTCC TGCTCACATG GGAGGTGCCA GAAACATACA AGTCTCAAGT GCCTTTCAAG 
ATCCTGTATA ACCAGCAGAG TGTGGAGGTC CAGGGTGACC TGAAGAGGAA GCTGATTACG 
CGTCTGCAGC CGGACACTGA TTACTCCTTT GTCCTGATGA GTCGGGGGAA CAGCGCTGGC 
GGACTGCAGC AGCAGGTCTC CATCCGCACC GCTCCAGACC TGTTCACTAC CAAACCAGCA 
CACCAGAAAC TAGACCCAGA GGAGGGCGGC AAACTCGCCA TCACACTGCC CGAAGTGCAC 
AACAGCGCCC TCGTCAGGTG GTTTTATATC GTGGTTGTAC CAGTAACTCC TTCAACTCTC 
CGCCATTGGG AAAACCCAGA AGATATTGAT CTGGATGAGC TGATGGGGAA TAATAGAGAC 
GCAGCTGTGA GGAGGAGGAG AAGACAAACT CAGGACTTTC TGCGGCCTTA CATTGCTGCC 
AAGCTGGAGT CTCTTCCAGA AAGATTTGTG TTGGGAGATG AGAAAACCTA CAGCAACTAC 
TACAACAAAC CTCTGCCGGG ACAGCAGCAG TATCGCACAT TTATACTGGC GGCACTGAAA 
GACAGAGACT CGACGTTCAC AGTGAGTCCG TTCTCTGAGC CCATGATGGT GAAGCTCCAC 
AGCGGGATGT CCCGTCACGT GGAGGACCCG GAGATGCTGT GGGTGATGGG ACCAGTGTTA 
GCCGTGGTCC TCATCATCAT CATCGTCATC GCCATCCTCC TCTTCAAGAG CAAACAGGAG 
AGGAAGCGCA CGTCTCCCTC AGCTAAAGAT GAGCACTCAG CTGGAGTTAA AGATCACCTG 
CTGGCTCATT CGTCAGATCC AGTGGAGATG AGGAGGCTCA ACTACCAGAC ACAAGGTTCC 
AGTGCCTTCA ACTGTCCCAG CACTTCAAGA ATGCGGGAAC ACCCTCCAGT GTCTGTGTGT 
GCTTTGGCTG ACCACATCGA GCGGCTGAGA GCCAACGACA GCCTCCGCTT CTCGCAGGAG 
TATGAGTCTG TTGACCCTGG CCAGCAGTTC ACCTGGGAAA ACTCCAACTT AGAGGTCAAC 
AAGCCCAAGA ACCGCTACGC AAACGTCATC GCTTACGATC ACTCCAGGGT TGTGCTGACT 
CCTGTGGATG GTGTTCCCGG AAGTGACTAC ATCAATGGAA ACTACATTGA TGGCTACCGT 
AAGCAGAACG CCTACATCGC CACGCAGGGT CCGCTGCCTG AGACGCTCAG TGACTTCTGG 
AGGATGGTGT GGGAACAGAG AACCAGCACT ATCGTCATGA TGACCCGACT GGAGGAGAAG 
AGCAGGGTGA AGTGTGATCA GTACTGGCCA AGCCGAGGAA CAGAGACTTA CGGGATGATT 
CAAGTGTCCA TGCTGGATAC GGTGGAGCTG GCCACATACA GCGTCAGAAC CTTCGCTCTC 
TACAAGAATG GATCCAGCGA GAAACGGGAG GTTCGGCAGT TCCAGTTCAT GGCGTGGCCG 
GATCACGGTG TTCCCGAATA TCCCACACCC ATTTTAGCCT TCTTACGCAG GGTCAAAGCC 
TGCAACCCAC CTGACGCGGG ACCCATGGTG GTGCACTGCA GCGCTGGCGT GGGTCGGACG 
GGCTGTTTCA TCGTAATAGA CGCCATGCTG GAGCGGATGA AGCACGAGAA GTCGGTGGAC 
ATCTACGGTC ACGTGACCTG CATGCGTTCT CAGAGGAACT ACATGGTGCA GACAGAGGAT 
CAGTACATTT TCATTCACGA GGCCCTGCTG GAGGCTGCCA CCTGCGGGAA CACAGAGGTG 
CCGGCGCGGA GCCTCTACAC ACACATCCAG AAACTCACAC AGGCGCCGCC TGGAGACACC 
GTCACCGCCA TGGAGCTCGA ATTTAAGAAA CTGGCCAACT CTAAAGCACA CACCTCCAGA 
TTCATCAGCG CTAGTCTGCC CTGCAACAAA TTCAAGAACC GGCTGGTGAA CATCATGCCT 
TTCGAGTCCA GCCGTGTTTG TCTGCAGCCA ATCAGAGGCG TGGAGGGCTC CGACTACATC 
AACGCCAGCT TCATCGACGG ATACAGGCAG CAGAAGGCAT ATATCGCCAC TCAAGGCCCG 
CTGTCTGAAA CCACAGAGGA CTTCTGGAGG ATGCTTTGGG AGCACAACTC CACCATCGTG 
GTGATGCTGA CGAAGCTTAG AGAGATGGGA CGGGAGAAAT GTCATCAGTA CTGGCCTGCA 
GAGAGATCTG CGCGCTACCA GTATTTCGTT GTGGATCCTA TGGCTGAATA TAACATGCCA 
CAGTACATCT TACGGGAGTT TAAAGTCACA GACGCCAGAG ATGGCCAGTC TCGGACTATC 
CGACAGTTTC AGTTCACAGA TTGGCCGGAA CAAGGTGTGC CAAAGACAGG AGAGGGCTTT 
ATTGATTTTA TTGGTCAAGT CCACAAAACC AAGGAGCAGT TTGGACAGGA CGGACCAATT 
ACTGTTCACT GCAGTGCCGG TGTGGGCAGA ACAGGCGTCT TCATCACTTT AAGTATTGTT 
CTGGAGCGGA TGAGGTATGA AGGAGTGGTG GACATGTTCC AGACCATAAA AACACTCCGC 
ACGCAGAGAC CTGCCATGGT ACAGACCGAG GACCAGTACC AGCTGTGCTA CAGAGCAGCT 
CTGGAGTACC TGGGCAGCTT CGATCACTAT GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfa ( XM_021476765.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332440.1 Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa142906
Clone ID Related Accession (Same CDS sequence)	XM_021476765.1
Accession Version	XM_021476765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGA GCGCTGCAGA TCGAGAACAG CGAGGAATCA 
GACCAGGGCA AGTATGAATG TGTGGCGGTG AACAGCGCGG GAACTCGATA CTCGGCTCCT 
GCAAACCTCT ACGTCAGAGT CCGACGGGTT CCCCCACGTT TTTCAATCCC TCCAACCAAT 
CACGAGGTGA TGCCGGGTGG CAGTGTGAAC CTCACCTGCG TGGCAGTGGG CGCTCCAATG 
CCCTATGTGA AGTGGATGAA GGGCGAACAG GAGCTGACCA AAGAAGAGGA GATGCCCATC 
GGCCGAAATG TGCTGGAGCT CACCAACATC CGCCAGTCGG GGAACTACAC CTGCGTGGCC 
ATATCATCAC TGGGCATGAT CGACACCACA GCGCAGATCA CTGTTAAAGC TCTTCCTCGT 
CCTCCAGCCT CTCTGATTGT CACTGAAACC ACCGCCACCA GTGTGACTTT AACATGGGAT 
TCTGGCAACC CAGAACCCGT ATCATATTAC GTGATCCAGT ATCGATCCAA AATCTCTGAC 
AATGGTTTCC AGGAGGTGGA CGGCGTGGCA TCCACCCGCT ACAGCATCGG CGGGTTGAGT 
CCGTACTCAG AGTATGAGTT TCGAGTAATG GCGGTCAATA ACATTGGTCG CGGGCCGCCC 
AGCGGGATGG TGGAGACTCG TACCAGTGAA CAGGCACCGT CATCTCCTCC GCTGCGCGTT 
CAAGCCCGGA TGTTGAGCGC TACGACTATG CTGGTGCAAT GGGAACCTCC TGAAGAGCCC 
AACGGACAGA TTCGAGGATA TCGAGTGTAC TACACGTCTG ACCTGCACTT CCCGCTCAGC 
ACATGGCAGA AGCACAACAC TGAGGACAGC AGTCTGACCA CCATCTCCGG CCTGGTTCCT 
GACATCACGT ATGGGCTCCG TGTGCTGGCC TTCACCTCTG TTGGAGATGG ACCACCTTCC 
GATATACTGC AGGTTAAAAC ACAGCAAGGA GTCCCTGCTC AGCCGACTGG TTTTGAAGCT 
GAAGCCGAGT TGGACACGCG GATCATGCTG TCATGGTTGT GGCCCGTCCA AGATCAGATC 
ACCAAATATG AGCTCACATA CTGGGAGGCT GACTCTGACA ACAAGCATCA TGTCATCTTT 
GACCCGGCCG GTTCATATGC TATTGACAGC CTAAAGCCAG ACACTCTCTA TAAATTCTCT 
CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG GTTTACACAC AGCCTATCGA GGCCCGCACA 
GCCCAGTCCG CCCCGTCCGC TCCCCCACAA GAGGTGCACC TGATCAGCCT GAGCTCCAGC 
AGCATCAAGG TGAGTTGGGT GGCCCCTCCG GCCGCCAGCC GGCACGGCAG CATCGTCCGC 
TACTCTCTGG CCTATCAGGC AGTGTCTGGC GAGGACCAGG AGCGCCACGA GGTCAAAGAC 
ATCCCTGCTG ATGTGTCCAG CTATGTTCTG GAGGGTCTGG AGAAATGGAC TGAGTATACG 
GTGTGGGTCA AAGCGCACAC AGATGTGGGT CCCGGGCCAG AAAGTGGCAG CACCCGCATC 
AGAACCCAAG AGGATGTGCC TGGTGCTCCT CCACGCAGGG TTGAGGTGGA CAACATCAAC 
TCCACTGCAC TGCGTGTGTC CTGGAAGCCG CCTCTGCAGC AGAAACAACA CGGCCACATA 
CGTGGATATC AGGTGGTTTA CTCACGCCTG GAGAATGGAG AGCCGCGCGG ACAGCCCATC 
ATTCTGGACG TGGCGCTGCC AGAGGCACAG GAGGCCGTGA TCAGTGGTCT TCTCCCTGAA 
ACCACATATT CAGTGACTGT AGCCGCGTAC ACCACTAAAG GAGATGGAGC GCGGAGCAAA 
GCCAAAGTGG TGACCACAAC AGGAGCAGTT CCAGGAAAGC CCACTATGAT CATCAGCACC 
ACCATAGGCA ACACGGCTCT GATCCAGTGG CAGCCGCCTA AAGAGATGGT GGGAGAGCTG 
ATGGGCTACA GACTCCGCTA TAAGCGTGAA GAAGAGGATA TTTACACTGT CCGAGACTTC 
AGACGCACTG ATGACCACTT CACTGTCACC GGCCTGCACA AGGGCGCGAC GTACATCTTC 
AAACTCTGCG CTAAAAACCG CGCCGGGAAT GGAGAGGAGT ACAGCAAAGA GATCAGCACC 
CCGGAGGATG TGCCCAGCAG TTACCCACAA AACCTCAAAG TCGTTGGATT GACCACAACC 
ACCACAAAAC TAGCCTGGGA TCCTCCGCCG CTGCCCGAGA GGAATGGGAA GATCGTACGA 
TATGTGGTGG TTTATCGAGA CATTAACAGC CATCAGAATC AGACCAACGG CACAGCGGAC 
ACGGAGATGA CCATTCAGGG CCTGAAGCCG GACACCACCT ATGATATCAG GGTGAGGGCG 
TTTACAGGGA AAGGTGGAGG TCCCATCAGC CCCAGCATCC AGAGCAGAAC CATGTCTACC 
TCCATGCCTG AGTTCACGAA AAACTTTGGT GTGAAAGCCG TGATGAAGAC CTCTGTCCTG 
CTCACATGGG AGGTGCCAGA AACATACAAG TCTCAAGTGC CTTTCAAGAT CCTGTATAAC 
CAGCAGAGTG TGGAGGTCCA GGGTGACCTG AAGAGGAAGC TGATTACGCG TCTGCAGCCG 
GACACTGATT ACTCCTTTGT CCTGATGAGT CGGGGGAACA GCGCTGGCGG ACTGCAGCAG 
CAGGTCTCCA TCCGCACCGC TCCAGACCTG TTCACTACCA AACCAGCACA CCAGAAACTA 
GACCCAGAGG AGGGCGGCAA ACTCGCCATC ACACTGCCCG AAGTGCACAA CAGCGCCCTC 
GTCAGGTGGT TTTATATCGT GGTTGTACCA GTAACTCCTT CAACTCTCCG CCATTGGGAA 
AACCCAGAAG ATATTGATCT GGATGAGCTG ATGGGGAATA ATAGAGACGC AGCTGTGAGG 
AGGAGGAGAA GACAAACTCA GGACTTTCTG CGGCCTTACA TTGCTGCCAA GCTGGAGTCT 
CTTCCAGAAA GATTTGTGTT GGGAGATGAG AAAACCTACA GCAACTACTA CAACAAACCT 
CTGCCGGGAC AGCAGCAGTA TCGCACATTT ATACTGGCGG CACTGAAAGA CAGAGACTCG 
ACGTTCACAG TGAGTCCGTT CTCTGAGCCC ATGATGGTGA AGCTCCACAG CGGGATGTCC 
CGTCACGTGG AGGACCCGGA GATGCTGTGG GTGATGGGAC CAGTGTTAGC CGTGGTCCTC 
ATCATCATCA TCGTCATCGC CATCCTCCTC TTCAAGAGCA AACAGGAGAG GAAGCGCACG 
TCTCCCTCAG CTAAAGATGA GCACTCAGCT GGAGTTAAAG ATCACCTGCT GGCTCATTCG 
TCAGATCCAG TGGAGATGAG GAGGCTCAAC TACCAGACAC AAGGTTCCAG TGCCTTCAAC 
TGTCCCAGCA CTTCAAGAAT GCGGGAACAC CCTCCAGTGT CTGTGTGTGC TTTGGCTGAC 
CACATCGAGC GGCTGAGAGC CAACGACAGC CTCCGCTTCT CGCAGGAGTA TGAGTCTGTT 
GACCCTGGCC AGCAGTTCAC CTGGGAAAAC TCCAACTTAG AGGTCAACAA GCCCAAGAAC 
CGCTACGCAA ACGTCATCGC TTACGATCAC TCCAGGGTTG TGCTGACTCC TGTGGATGGT 
GTTCCCGGAA GTGACTACAT CAATGGAAAC TACATTGATG GCTACCGTAA GCAGAACGCC 
TACATCGCCA CGCAGGGTCC GCTGCCTGAG ACGCTCAGTG ACTTCTGGAG GATGGTGTGG 
GAACAGAGAA CCAGCACTAT CGTCATGATG ACCCGACTGG AGGAGAAGAG CAGGGTGAAG 
TGTGATCAGT ACTGGCCAAG CCGAGGAACA GAGACTTACG GGATGATTCA AGTGTCCATG 
CTGGATACGG TGGAGCTGGC CACATACAGC GTCAGAACCT TCGCTCTCTA CAAGAATGGA 
TCCAGCGAGA AACGGGAGGT TCGGCAGTTC CAGTTCATGG CGTGGCCGGA TCACGGTGTT 
CCCGAATATC CCACACCCAT TTTAGCCTTC TTACGCAGGG TCAAAGCCTG CAACCCACCT 
GACGCGGGAC CCATGGTGGT GCACTGCAGC GCTGGCGTGG GTCGGACGGG CTGTTTCATC 
GTAATAGACG CCATGCTGGA GCGGATGAAG CACGAGAAGT CGGTGGACAT CTACGGTCAC 
GTGACCTGCA TGCGTTCTCA GAGGAACTAC ATGGTGCAGA CAGAGGATCA GTACATTTTC 
ATTCACGAGG CCCTGCTGGA GGCTGCCACC TGCGGGAACA CAGAGGTGCC GGCGCGGAGC 
CTCTACACAC ACATCCAGAA ACTCACACAG GCGCCGCCTG GAGACACCGT CACCGCCATG 
GAGCTCGAAT TTAAGAAACT GGCCAACTCT AAAGCACACA CCTCCAGATT CATCAGCGCT 
AGTCTGCCCT GCAACAAATT CAAGAACCGG CTGGTGAACA TCATGCCTTT CGAGTCCAGC 
CGTGTTTGTC TGCAGCCAAT CAGAGGCGTG GAGGGCTCCG ACTACATCAA CGCCAGCTTC 
ATCGACGGAT ACAGGCAGCA GAAGGCATAT ATCGCCACTC AAGGCCCGCT GTCTGAAACC 
ACAGAGGACT TCTGGAGGAT GCTTTGGGAG CACAACTCCA CCATCGTGGT GATGCTGACG 
AAGCTTAGAG AGATGGGACG GGAGAAATGT CATCAGTACT GGCCTGCAGA GAGATCTGCG 
CGCTACCAGT ATTTCGTTGT GGATCCTATG GCTGAATATA ACATGCCACA GTACATCTTA 
CGGGAGTTTA AAGTCACAGA CGCCAGAGAT GGCCAGTCTC GGACTATCCG ACAGTTTCAG 
TTCACAGATT GGCCGGAACA AGGTGTGCCA AAGACAGGAG AGGGCTTTAT TGATTTTATT 
GGTCAAGTCC ACAAAACCAA GGAGCAGTTT GGACAGGACG GACCAATTAC TGTTCACTGC 
AGTGCCGGTG TGGGCAGAAC AGGCGTCTTC ATCACTTTAA GTATTGTTCT GGAGCGGATG 
AGGTATGAAG GAGTGGTGGA CATGTTCCAG ACCATAAAAA CACTCCGCAC GCAGAGACCT 
GCCATGGTAC AGACCGAGGA CCAGTACCAG CTGTGCTACA GAGCAGCTCT GGAGTACCTG 
GGCAGCTTCG ATCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332440.1
CDS	1458..7184
Translation	MVPQTRQGSARHGATSLLLLLCLAVPIHADSMPSFVKTPEDQTGISGGVASFVCQAVGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEEDQIPHGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEISWFKDFLPVDINSSNGRIKQLRSGALQIENSEESDQGKYECVAVNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMKGEQELTKEEEMPIGRNVLELTNIRQSGNYTCVAISSLGMIDTTAQITVKALPRPPASLIVTETTATSVTLTWDSGNPEPVSYYVIQYRSKISDNGFQEVDGVASTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSGMVETRTSEQAPSSPPLRVQARMLSATTMLVQWEPPEEPNGQIRGYRVYYTSDLHFPLSTWQKHNTEDSSLTTISGLVPDITYGLRVLAFTSVGDGPPSDILQVKTQQGVPAQPTGFEAEAELDTRIMLSWLWPVQDQITKYELTYWEADSDNKHHVIFDPAGSYAIDSLKPDTLYKFSLAARSEMGLGVYTQPIEARTAQSAPSAPPQEVHLISLSSSSIKVSWVAPPAASRHGSIVRYSLAYQAVSGEDQERHEVKDIPADVSSYVLEGLEKWTEYTVWVKAHTDVGPGPESGSTRIRTQEDVPGAPPRRVEVDNINSTALRVSWKPPLQQKQHGHIRGYQVVYSRLENGEPRGQPIILDVALPEAQEAVISGLLPETTYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTIGNTALIQWQPPKEMVGELMGYRLRYKREEEDIYTVRDFRRTDDHFTVTGLHKGATYIFKLCAKNRAGNGEEYSKEISTPEDVPSSYPQNLKVVGLTTTTTKLAWDPPPLPERNGKIVRYVVVYRDINSHQNQTNGTADTEMTIQGLKPDTTYDIRVRAFTGKGGGPISPSIQSRTMSTSMPEFTKNFGVKAVMKTSVLLTWEVPETYKSQVPFKILYNQQSVEVQGDLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFTTKPAHQKLDPEEGGKLAITLPEVHNSALVRWFYIVVVPVTPSTLRHWENPEDIDLDELMGNNRDAAVRRRRRQTQDFLRPYIAAKLESLPERFVLGDEKTYSNYYNKPLPGQQQYRTFILAALKDRDSTFTVSPFSEPMMVKLHSGMSRHVEDPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRTSPSAKDEHSAGVKDHLLAHSSDPVEMRRLNYQTQGSSAFNCPSTSRMREHPPVSVCALADHIERLRANDSLRFSQEYESVDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINGNYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRTSTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVSMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARSLYTHIQKLTQAPPGDTVTAMELEFKKLANSKAHTSRFISASLPCNKFKNRLVNIMPFESSRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLSETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTIKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021476765.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476765.1

ORF Insert Sequence:

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGA GCGCTGCAGA TCGAGAACAG CGAGGAATCA 
GACCAGGGCA AGTATGAATG TGTGGCGGTG AACAGCGCGG GAACTCGATA CTCGGCTCCT 
GCAAACCTCT ACGTCAGAGT CCGACGGGTT CCCCCACGTT TTTCAATCCC TCCAACCAAT 
CACGAGGTGA TGCCGGGTGG CAGTGTGAAC CTCACCTGCG TGGCAGTGGG CGCTCCAATG 
CCCTATGTGA AGTGGATGAA GGGCGAACAG GAGCTGACCA AAGAAGAGGA GATGCCCATC 
GGCCGAAATG TGCTGGAGCT CACCAACATC CGCCAGTCGG GGAACTACAC CTGCGTGGCC 
ATATCATCAC TGGGCATGAT CGACACCACA GCGCAGATCA CTGTTAAAGC TCTTCCTCGT 
CCTCCAGCCT CTCTGATTGT CACTGAAACC ACCGCCACCA GTGTGACTTT AACATGGGAT 
TCTGGCAACC CAGAACCCGT ATCATATTAC GTGATCCAGT ATCGATCCAA AATCTCTGAC 
AATGGTTTCC AGGAGGTGGA CGGCGTGGCA TCCACCCGCT ACAGCATCGG CGGGTTGAGT 
CCGTACTCAG AGTATGAGTT TCGAGTAATG GCGGTCAATA ACATTGGTCG CGGGCCGCCC 
AGCGGGATGG TGGAGACTCG TACCAGTGAA CAGGCACCGT CATCTCCTCC GCTGCGCGTT 
CAAGCCCGGA TGTTGAGCGC TACGACTATG CTGGTGCAAT GGGAACCTCC TGAAGAGCCC 
AACGGACAGA TTCGAGGATA TCGAGTGTAC TACACGTCTG ACCTGCACTT CCCGCTCAGC 
ACATGGCAGA AGCACAACAC TGAGGACAGC AGTCTGACCA CCATCTCCGG CCTGGTTCCT 
GACATCACGT ATGGGCTCCG TGTGCTGGCC TTCACCTCTG TTGGAGATGG ACCACCTTCC 
GATATACTGC AGGTTAAAAC ACAGCAAGGA GTCCCTGCTC AGCCGACTGG TTTTGAAGCT 
GAAGCCGAGT TGGACACGCG GATCATGCTG TCATGGTTGT GGCCCGTCCA AGATCAGATC 
ACCAAATATG AGCTCACATA CTGGGAGGCT GACTCTGACA ACAAGCATCA TGTCATCTTT 
GACCCGGCCG GTTCATATGC TATTGACAGC CTAAAGCCAG ACACTCTCTA TAAATTCTCT 
CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG GTTTACACAC AGCCTATCGA GGCCCGCACA 
GCCCAGTCCG CCCCGTCCGC TCCCCCACAA GAGGTGCACC TGATCAGCCT GAGCTCCAGC 
AGCATCAAGG TGAGTTGGGT GGCCCCTCCG GCCGCCAGCC GGCACGGCAG CATCGTCCGC 
TACTCTCTGG CCTATCAGGC AGTGTCTGGC GAGGACCAGG AGCGCCACGA GGTCAAAGAC 
ATCCCTGCTG ATGTGTCCAG CTATGTTCTG GAGGGTCTGG AGAAATGGAC TGAGTATACG 
GTGTGGGTCA AAGCGCACAC AGATGTGGGT CCCGGGCCAG AAAGTGGCAG CACCCGCATC 
AGAACCCAAG AGGATGTGCC TGGTGCTCCT CCACGCAGGG TTGAGGTGGA CAACATCAAC 
TCCACTGCAC TGCGTGTGTC CTGGAAGCCG CCTCTGCAGC AGAAACAACA CGGCCACATA 
CGTGGATATC AGGTGGTTTA CTCACGCCTG GAGAATGGAG AGCCGCGCGG ACAGCCCATC 
ATTCTGGACG TGGCGCTGCC AGAGGCACAG GAGGCCGTGA TCAGTGGTCT TCTCCCTGAA 
ACCACATATT CAGTGACTGT AGCCGCGTAC ACCACTAAAG GAGATGGAGC GCGGAGCAAA 
GCCAAAGTGG TGACCACAAC AGGAGCAGTT CCAGGAAAGC CCACTATGAT CATCAGCACC 
ACCATAGGCA ACACGGCTCT GATCCAGTGG CAGCCGCCTA AAGAGATGGT GGGAGAGCTG 
ATGGGCTACA GACTCCGCTA TAAGCGTGAA GAAGAGGATA TTTACACTGT CCGAGACTTC 
AGACGCACTG ATGACCACTT CACTGTCACC GGCCTGCACA AGGGCGCGAC GTACATCTTC 
AAACTCTGCG CTAAAAACCG CGCCGGGAAT GGAGAGGAGT ACAGCAAAGA GATCAGCACC 
CCGGAGGATG TGCCCAGCAG TTACCCACAA AACCTCAAAG TCGTTGGATT GACCACAACC 
ACCACAAAAC TAGCCTGGGA TCCTCCGCCG CTGCCCGAGA GGAATGGGAA GATCGTACGA 
TATGTGGTGG TTTATCGAGA CATTAACAGC CATCAGAATC AGACCAACGG CACAGCGGAC 
ACGGAGATGA CCATTCAGGG CCTGAAGCCG GACACCACCT ATGATATCAG GGTGAGGGCG 
TTTACAGGGA AAGGTGGAGG TCCCATCAGC CCCAGCATCC AGAGCAGAAC CATGTCTACC 
TCCATGCCTG AGTTCACGAA AAACTTTGGT GTGAAAGCCG TGATGAAGAC CTCTGTCCTG 
CTCACATGGG AGGTGCCAGA AACATACAAG TCTCAAGTGC CTTTCAAGAT CCTGTATAAC 
CAGCAGAGTG TGGAGGTCCA GGGTGACCTG AAGAGGAAGC TGATTACGCG TCTGCAGCCG 
GACACTGATT ACTCCTTTGT CCTGATGAGT CGGGGGAACA GCGCTGGCGG ACTGCAGCAG 
CAGGTCTCCA TCCGCACCGC TCCAGACCTG TTCACTACCA AACCAGCACA CCAGAAACTA 
GACCCAGAGG AGGGCGGCAA ACTCGCCATC ACACTGCCCG AAGTGCACAA CAGCGCCCTC 
GTCAGGTGGT TTTATATCGT GGTTGTACCA GTAACTCCTT CAACTCTCCG CCATTGGGAA 
AACCCAGAAG ATATTGATCT GGATGAGCTG ATGGGGAATA ATAGAGACGC AGCTGTGAGG 
AGGAGGAGAA GACAAACTCA GGACTTTCTG CGGCCTTACA TTGCTGCCAA GCTGGAGTCT 
CTTCCAGAAA GATTTGTGTT GGGAGATGAG AAAACCTACA GCAACTACTA CAACAAACCT 
CTGCCGGGAC AGCAGCAGTA TCGCACATTT ATACTGGCGG CACTGAAAGA CAGAGACTCG 
ACGTTCACAG TGAGTCCGTT CTCTGAGCCC ATGATGGTGA AGCTCCACAG CGGGATGTCC 
CGTCACGTGG AGGACCCGGA GATGCTGTGG GTGATGGGAC CAGTGTTAGC CGTGGTCCTC 
ATCATCATCA TCGTCATCGC CATCCTCCTC TTCAAGAGCA AACAGGAGAG GAAGCGCACG 
TCTCCCTCAG CTAAAGATGA GCACTCAGCT GGAGTTAAAG ATCACCTGCT GGCTCATTCG 
TCAGATCCAG TGGAGATGAG GAGGCTCAAC TACCAGACAC AAGGTTCCAG TGCCTTCAAC 
TGTCCCAGCA CTTCAAGAAT GCGGGAACAC CCTCCAGTGT CTGTGTGTGC TTTGGCTGAC 
CACATCGAGC GGCTGAGAGC CAACGACAGC CTCCGCTTCT CGCAGGAGTA TGAGTCTGTT 
GACCCTGGCC AGCAGTTCAC CTGGGAAAAC TCCAACTTAG AGGTCAACAA GCCCAAGAAC 
CGCTACGCAA ACGTCATCGC TTACGATCAC TCCAGGGTTG TGCTGACTCC TGTGGATGGT 
GTTCCCGGAA GTGACTACAT CAATGGAAAC TACATTGATG GCTACCGTAA GCAGAACGCC 
TACATCGCCA CGCAGGGTCC GCTGCCTGAG ACGCTCAGTG ACTTCTGGAG GATGGTGTGG 
GAACAGAGAA CCAGCACTAT CGTCATGATG ACCCGACTGG AGGAGAAGAG CAGGGTGAAG 
TGTGATCAGT ACTGGCCAAG CCGAGGAACA GAGACTTACG GGATGATTCA AGTGTCCATG 
CTGGATACGG TGGAGCTGGC CACATACAGC GTCAGAACCT TCGCTCTCTA CAAGAATGGA 
TCCAGCGAGA AACGGGAGGT TCGGCAGTTC CAGTTCATGG CGTGGCCGGA TCACGGTGTT 
CCCGAATATC CCACACCCAT TTTAGCCTTC TTACGCAGGG TCAAAGCCTG CAACCCACCT 
GACGCGGGAC CCATGGTGGT GCACTGCAGC GCTGGCGTGG GTCGGACGGG CTGTTTCATC 
GTAATAGACG CCATGCTGGA GCGGATGAAG CACGAGAAGT CGGTGGACAT CTACGGTCAC 
GTGACCTGCA TGCGTTCTCA GAGGAACTAC ATGGTGCAGA CAGAGGATCA GTACATTTTC 
ATTCACGAGG CCCTGCTGGA GGCTGCCACC TGCGGGAACA CAGAGGTGCC GGCGCGGAGC 
CTCTACACAC ACATCCAGAA ACTCACACAG GCGCCGCCTG GAGACACCGT CACCGCCATG 
GAGCTCGAAT TTAAGAAACT GGCCAACTCT AAAGCACACA CCTCCAGATT CATCAGCGCT 
AGTCTGCCCT GCAACAAATT CAAGAACCGG CTGGTGAACA TCATGCCTTT CGAGTCCAGC 
CGTGTTTGTC TGCAGCCAAT CAGAGGCGTG GAGGGCTCCG ACTACATCAA CGCCAGCTTC 
ATCGACGGAT ACAGGCAGCA GAAGGCATAT ATCGCCACTC AAGGCCCGCT GTCTGAAACC 
ACAGAGGACT TCTGGAGGAT GCTTTGGGAG CACAACTCCA CCATCGTGGT GATGCTGACG 
AAGCTTAGAG AGATGGGACG GGAGAAATGT CATCAGTACT GGCCTGCAGA GAGATCTGCG 
CGCTACCAGT ATTTCGTTGT GGATCCTATG GCTGAATATA ACATGCCACA GTACATCTTA 
CGGGAGTTTA AAGTCACAGA CGCCAGAGAT GGCCAGTCTC GGACTATCCG ACAGTTTCAG 
TTCACAGATT GGCCGGAACA AGGTGTGCCA AAGACAGGAG AGGGCTTTAT TGATTTTATT 
GGTCAAGTCC ACAAAACCAA GGAGCAGTTT GGACAGGACG GACCAATTAC TGTTCACTGC 
AGTGCCGGTG TGGGCAGAAC AGGCGTCTTC ATCACTTTAA GTATTGTTCT GGAGCGGATG 
AGGTATGAAG GAGTGGTGGA CATGTTCCAG ACCATAAAAA CACTCCGCAC GCAGAGACCT 
GCCATGGTAC AGACCGAGGA CCAGTACCAG CTGTGCTACA GAGCAGCTCT GGAGTACCTG 
GGCAGCTTCG ATCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfa ( XM_021476766.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332441.1 Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X7, mRNA.

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CloneID	ODa142907
Clone ID Related Accession (Same CDS sequence)	XM_021476766.1
Accession Version	XM_021476766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGACC AGCGGGAAGT CCGACGGGTT 
CCCCCACGTT TTTCAATCCC TCCAACCAAT CACGAGGTGA TGCCGGGTGG CAGTGTGAAC 
CTCACCTGCG TGGCAGTGGG CGCTCCAATG CCCTATGTGA AGTGGATGAA GGGCGAACAG 
GAGCTGACCA AAGAAGAGGA GATGCCCATC GGCCGAAATG TGCTGGAGCT CACCAACATC 
CGCCAGTCGG GGAACTACAC CTGCGTGGCC ATATCATCAC TGGGCATGAT CGACACCACA 
GCGCAGATCA CTGTTAAAGC TCTTCCTCGT CCTCCAGCCT CTCTGATTGT CACTGAAACC 
ACCGCCACCA GTGTGACTTT AACATGGGAT TCTGGCAACC CAGAACCCGT ATCATATTAC 
GTGATCCAGT ATCGATCCAA AATCTCTGAC AATGGTTTCC AGGAGGTGGA CGGCGTGGCA 
TCCACCCGCT ACAGCATCGG CGGGTTGAGT CCGTACTCAG AGTATGAGTT TCGAGTAATG 
GCGGTCAATA ACATTGGTCG CGGGCCGCCC AGCGGGATGG TGGAGACTCG TACCAGTGAA 
CAGGCACCGT CATCTCCTCC GCTGCGCGTT CAAGCCCGGA TGTTGAGCGC TACGACTATG 
CTGGTGCAAT GGGAACCTCC TGAAGAGCCC AACGGACAGA TTCGAGGATA TCGAGTGTAC 
TACACGTCTG ACCTGCACTT CCCGCTCAGC ACATGGCAGA AGCACAACAC TGAGGACAGC 
AGTCTGACCA CCATCTCCGG CCTGGTTCCT GACATCACGT ATGGGCTCCG TGTGCTGGCC 
TTCACCTCTG TTGGAGATGG ACCACCTTCC GATATACTGC AGGTTAAAAC ACAGCAAGGA 
GTCCCTGCTC AGCCGACTGG TTTTGAAGCT GAAGCCGAGT TGGACACGCG GATCATGCTG 
TCATGGTTGT GGCCCGTCCA AGATCAGATC ACCAAATATG AGCTCACATA CTGGGAGGCT 
GACTCTGACA ACAAGCATCA TGTCATCTTT GACCCGGCCG GTTCATATGC TATTGACAGC 
CTAAAGCCAG ACACTCTCTA TAAATTCTCT CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG 
GTTTACACAC AGCCTATCGA GGCCCGCACA GCCCAGTCCG CCCCGTCCGC TCCCCCACAA 
GAGGTGCACC TGATCAGCCT GAGCTCCAGC AGCATCAAGG TGAGTTGGGT GGCCCCTCCG 
GCCGCCAGCC GGCACGGCAG CATCGTCCGC TACTCTCTGG CCTATCAGGC AGTGTCTGGC 
GAGGACCAGG AGCGCCACGA GGTCAAAGAC ATCCCTGCTG ATGTGTCCAG CTATGTTCTG 
GAGGGTCTGG AGAAATGGAC TGAGTATACG GTGTGGGTCA AAGCGCACAC AGATGTGGGT 
CCCGGGCCAG AAAGTGGCAG CACCCGCATC AGAACCCAAG AGGATGTGCC TGGTGCTCCT 
CCACGCAGGG TTGAGGTGGA CAACATCAAC TCCACTGCAC TGCGTGTGTC CTGGAAGCCG 
CCTCTGCAGC AGAAACAACA CGGCCACATA CGTGGATATC AGGTGGTTTA CTCACGCCTG 
GAGAATGGAG AGCCGCGCGG ACAGCCCATC ATTCTGGACG TGGCGCTGCC AGAGGCACAG 
GAGGCCGTGA TCAGTGGTCT TCTCCCTGAA ACCACATATT CAGTGACTGT AGCCGCGTAC 
ACCACTAAAG GAGATGGAGC GCGGAGCAAA GCCAAAGTGG TGACCACAAC AGGAGCAGTT 
CCAGGAAAGC CCACTATGAT CATCAGCACC ACCATAGGCA ACACGGCTCT GATCCAGTGG 
CAGCCGCCTA AAGAGATGGT GGGAGAGCTG ATGGGCTACA GACTCCGCTA TAAGCGTGAA 
GAAGAGGATA TTTACACTGT CCGAGACTTC AGACGCACTG ATGACCACTT CACTGTCACC 
GGCCTGCACA AGGGCGCGAC GTACATCTTC AAACTCTGCG CTAAAAACCG CGCCGGGAAT 
GGAGAGGAGT ACAGCAAAGA GATCAGCACC CCGGAGGATG TGCCCAGCAG TTACCCACAA 
AACCTCAAAG TCGTTGGATT GACCACAACC ACCACAAAAC TAGCCTGGGA TCCTCCGCCG 
CTGCCCGAGA GGAATGGGAA GATCGTACGA TATGTGGTGG TTTATCGAGA CATTAACAGC 
CATCAGAATC AGACCAACGG CACAGCGGAC ACGGAGATGA CCATTCAGGG CCTGAAGCCG 
GACACCACCT ATGATATCAG GGTGAGGGCG TTTACAGGGA AAGGTGGAGG TCCCATCAGC 
CCCAGCATCC AGAGCAGAAC CATGTCTACC TCCATGCCTG AGTTCACGAA AAACTTTGGT 
GTGAAAGCCG TGATGAAGAC CTCTGTCCTG CTCACATGGG AGGTGCCAGA AACATACAAG 
TCTCAAGTGC CTTTCAAGAT CCTGTATAAC CAGCAGAGTG TGGAGGTCCA GGGTGACCTG 
AAGAGGAAGC TGATTACGCG TCTGCAGCCG GACACTGATT ACTCCTTTGT CCTGATGAGT 
CGGGGGAACA GCGCTGGCGG ACTGCAGCAG CAGGTCTCCA TCCGCACCGC TCCAGACCTG 
TTCACTACCA AACCAGCACA CCAGAAACTA GACCCAGAGG AGGGCGGCAA ACTCGCCATC 
ACACTGCCCG AAGTGCACAA CAGCGCCCTC GTCAGGTGGT TTTATATCGT GGTTGTACCA 
GTAACTCCTT CAACTCTCCG CCATTGGGAA AACCCAGAAG ATATTGATCT GGATGAGCTG 
ATGGGGAATA ATAGAGACGC AGCTGTGAGG AGGAGGAGAA GACAAACTCA GGACTTTCTG 
CGGCCTTACA TTGCTGCCAA GCTGGAGTCT CTTCCAGAAA GATTTGTGTT GGGAGATGAG 
AAAACCTACA GCAACTACTA CAACAAACCT CTGCCGGGAC AGCAGCAGTA TCGCACATTT 
ATACTGGCGG CACTGAAAGA CAGAGACTCG ACGTTCACAG TGAGTCCGTT CTCTGAGCCC 
ATGATGGTGA AGCTCCACAG CGGGATGTCC CGTCACGTGG AGGACCCGGA GATGCTGTGG 
GTGATGGGAC CAGTGTTAGC CGTGGTCCTC ATCATCATCA TCGTCATCGC CATCCTCCTC 
TTCAAGAGCA AACAGGAGAG GAAGCGCACG TCTCCCTCAG CTAAAGATGA GCACTCAGCT 
GGAGTTAAAG ATCACCTGCT GGCTCATTCG TCAGATCCAG TGGAGATGAG GAGGCTCAAC 
TACCAGACAC AAGGAATGCG GGAACACCCT CCAGTGTCTG TGTGTGCTTT GGCTGACCAC 
ATCGAGCGGC TGAGAGCCAA CGACAGCCTC CGCTTCTCGC AGGAGTATGA GTCTGTTGAC 
CCTGGCCAGC AGTTCACCTG GGAAAACTCC AACTTAGAGG TCAACAAGCC CAAGAACCGC 
TACGCAAACG TCATCGCTTA CGATCACTCC AGGGTTGTGC TGACTCCTGT GGATGGTGTT 
CCCGGAAGTG ACTACATCAA TGGAAACTAC ATTGATGGCT ACCGTAAGCA GAACGCCTAC 
ATCGCCACGC AGGGTCCGCT GCCTGAGACG CTCAGTGACT TCTGGAGGAT GGTGTGGGAA 
CAGAGAACCA GCACTATCGT CATGATGACC CGACTGGAGG AGAAGAGCAG GGTGAAGTGT 
GATCAGTACT GGCCAAGCCG AGGAACAGAG ACTTACGGGA TGATTCAAGT GTCCATGCTG 
GATACGGTGG AGCTGGCCAC ATACAGCGTC AGAACCTTCG CTCTCTACAA GAATGGATCC 
AGCGAGAAAC GGGAGGTTCG GCAGTTCCAG TTCATGGCGT GGCCGGATCA CGGTGTTCCC 
GAATATCCCA CACCCATTTT AGCCTTCTTA CGCAGGGTCA AAGCCTGCAA CCCACCTGAC 
GCGGGACCCA TGGTGGTGCA CTGCAGCGCT GGCGTGGGTC GGACGGGCTG TTTCATCGTA 
ATAGACGCCA TGCTGGAGCG GATGAAGCAC GAGAAGTCGG TGGACATCTA CGGTCACGTG 
ACCTGCATGC GTTCTCAGAG GAACTACATG GTGCAGACAG AGGATCAGTA CATTTTCATT 
CACGAGGCCC TGCTGGAGGC TGCCACCTGC GGGAACACAG AGGTGCCGGC GCGGAGCCTC 
TACACACACA TCCAGAAACT CACACAGGCG CCGCCTGGAG ACACCGTCAC CGCCATGGAG 
CTCGAATTTA AGAAACTGGC CAACTCTAAA GCACACACCT CCAGATTCAT CAGCGCTAGT 
CTGCCCTGCA ACAAATTCAA GAACCGGCTG GTGAACATCA TGCCTTTCGA GTCCAGCCGT 
GTTTGTCTGC AGCCAATCAG AGGCGTGGAG GGCTCCGACT ACATCAACGC CAGCTTCATC 
GACGGATACA GGCAGCAGAA GGCATATATC GCCACTCAAG GCCCGCTGTC TGAAACCACA 
GAGGACTTCT GGAGGATGCT TTGGGAGCAC AACTCCACCA TCGTGGTGAT GCTGACGAAG 
CTTAGAGAGA TGGGACGGGA GAAATGTCAT CAGTACTGGC CTGCAGAGAG ATCTGCGCGC 
TACCAGTATT TCGTTGTGGA TCCTATGGCT GAATATAACA TGCCACAGTA CATCTTACGG 
GAGTTTAAAG TCACAGACGC CAGAGATGGC CAGTCTCGGA CTATCCGACA GTTTCAGTTC 
ACAGATTGGC CGGAACAAGG TGTGCCAAAG ACAGGAGAGG GCTTTATTGA TTTTATTGGT 
CAAGTCCACA AAACCAAGGA GCAGTTTGGA CAGGACGGAC CAATTACTGT TCACTGCAGT 
GCCGGTGTGG GCAGAACAGG CGTCTTCATC ACTTTAAGTA TTGTTCTGGA GCGGATGAGG 
TATGAAGGAG TGGTGGACAT GTTCCAGACC ATAAAAACAC TCCGCACGCA GAGACCTGCC 
ATGGTACAGA CCGAGGACCA GTACCAGCTG TGCTACAGAG CAGCTCTGGA GTACCTGGGC 
AGCTTCGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332441.1
CDS	1458..7181
Translation	MVPQTRQGSARHGATSLLLLLCLAVPIHADSMPSFVKTPEDQTGISGGVASFVCQAVGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEEDQIPHGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEISWFKDFLPVDINSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAVNSAGTRYSAPANLYVRDQREVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMKGEQELTKEEEMPIGRNVLELTNIRQSGNYTCVAISSLGMIDTTAQITVKALPRPPASLIVTETTATSVTLTWDSGNPEPVSYYVIQYRSKISDNGFQEVDGVASTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSGMVETRTSEQAPSSPPLRVQARMLSATTMLVQWEPPEEPNGQIRGYRVYYTSDLHFPLSTWQKHNTEDSSLTTISGLVPDITYGLRVLAFTSVGDGPPSDILQVKTQQGVPAQPTGFEAEAELDTRIMLSWLWPVQDQITKYELTYWEADSDNKHHVIFDPAGSYAIDSLKPDTLYKFSLAARSEMGLGVYTQPIEARTAQSAPSAPPQEVHLISLSSSSIKVSWVAPPAASRHGSIVRYSLAYQAVSGEDQERHEVKDIPADVSSYVLEGLEKWTEYTVWVKAHTDVGPGPESGSTRIRTQEDVPGAPPRRVEVDNINSTALRVSWKPPLQQKQHGHIRGYQVVYSRLENGEPRGQPIILDVALPEAQEAVISGLLPETTYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTIGNTALIQWQPPKEMVGELMGYRLRYKREEEDIYTVRDFRRTDDHFTVTGLHKGATYIFKLCAKNRAGNGEEYSKEISTPEDVPSSYPQNLKVVGLTTTTTKLAWDPPPLPERNGKIVRYVVVYRDINSHQNQTNGTADTEMTIQGLKPDTTYDIRVRAFTGKGGGPISPSIQSRTMSTSMPEFTKNFGVKAVMKTSVLLTWEVPETYKSQVPFKILYNQQSVEVQGDLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFTTKPAHQKLDPEEGGKLAITLPEVHNSALVRWFYIVVVPVTPSTLRHWENPEDIDLDELMGNNRDAAVRRRRRQTQDFLRPYIAAKLESLPERFVLGDEKTYSNYYNKPLPGQQQYRTFILAALKDRDSTFTVSPFSEPMMVKLHSGMSRHVEDPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRTSPSAKDEHSAGVKDHLLAHSSDPVEMRRLNYQTQGMREHPPVSVCALADHIERLRANDSLRFSQEYESVDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINGNYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRTSTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVSMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARSLYTHIQKLTQAPPGDTVTAMELEFKKLANSKAHTSRFISASLPCNKFKNRLVNIMPFESSRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLSETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTIKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021476766.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476766.1

ORF Insert Sequence:

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGACC AGCGGGAAGT CCGACGGGTT 
CCCCCACGTT TTTCAATCCC TCCAACCAAT CACGAGGTGA TGCCGGGTGG CAGTGTGAAC 
CTCACCTGCG TGGCAGTGGG CGCTCCAATG CCCTATGTGA AGTGGATGAA GGGCGAACAG 
GAGCTGACCA AAGAAGAGGA GATGCCCATC GGCCGAAATG TGCTGGAGCT CACCAACATC 
CGCCAGTCGG GGAACTACAC CTGCGTGGCC ATATCATCAC TGGGCATGAT CGACACCACA 
GCGCAGATCA CTGTTAAAGC TCTTCCTCGT CCTCCAGCCT CTCTGATTGT CACTGAAACC 
ACCGCCACCA GTGTGACTTT AACATGGGAT TCTGGCAACC CAGAACCCGT ATCATATTAC 
GTGATCCAGT ATCGATCCAA AATCTCTGAC AATGGTTTCC AGGAGGTGGA CGGCGTGGCA 
TCCACCCGCT ACAGCATCGG CGGGTTGAGT CCGTACTCAG AGTATGAGTT TCGAGTAATG 
GCGGTCAATA ACATTGGTCG CGGGCCGCCC AGCGGGATGG TGGAGACTCG TACCAGTGAA 
CAGGCACCGT CATCTCCTCC GCTGCGCGTT CAAGCCCGGA TGTTGAGCGC TACGACTATG 
CTGGTGCAAT GGGAACCTCC TGAAGAGCCC AACGGACAGA TTCGAGGATA TCGAGTGTAC 
TACACGTCTG ACCTGCACTT CCCGCTCAGC ACATGGCAGA AGCACAACAC TGAGGACAGC 
AGTCTGACCA CCATCTCCGG CCTGGTTCCT GACATCACGT ATGGGCTCCG TGTGCTGGCC 
TTCACCTCTG TTGGAGATGG ACCACCTTCC GATATACTGC AGGTTAAAAC ACAGCAAGGA 
GTCCCTGCTC AGCCGACTGG TTTTGAAGCT GAAGCCGAGT TGGACACGCG GATCATGCTG 
TCATGGTTGT GGCCCGTCCA AGATCAGATC ACCAAATATG AGCTCACATA CTGGGAGGCT 
GACTCTGACA ACAAGCATCA TGTCATCTTT GACCCGGCCG GTTCATATGC TATTGACAGC 
CTAAAGCCAG ACACTCTCTA TAAATTCTCT CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG 
GTTTACACAC AGCCTATCGA GGCCCGCACA GCCCAGTCCG CCCCGTCCGC TCCCCCACAA 
GAGGTGCACC TGATCAGCCT GAGCTCCAGC AGCATCAAGG TGAGTTGGGT GGCCCCTCCG 
GCCGCCAGCC GGCACGGCAG CATCGTCCGC TACTCTCTGG CCTATCAGGC AGTGTCTGGC 
GAGGACCAGG AGCGCCACGA GGTCAAAGAC ATCCCTGCTG ATGTGTCCAG CTATGTTCTG 
GAGGGTCTGG AGAAATGGAC TGAGTATACG GTGTGGGTCA AAGCGCACAC AGATGTGGGT 
CCCGGGCCAG AAAGTGGCAG CACCCGCATC AGAACCCAAG AGGATGTGCC TGGTGCTCCT 
CCACGCAGGG TTGAGGTGGA CAACATCAAC TCCACTGCAC TGCGTGTGTC CTGGAAGCCG 
CCTCTGCAGC AGAAACAACA CGGCCACATA CGTGGATATC AGGTGGTTTA CTCACGCCTG 
GAGAATGGAG AGCCGCGCGG ACAGCCCATC ATTCTGGACG TGGCGCTGCC AGAGGCACAG 
GAGGCCGTGA TCAGTGGTCT TCTCCCTGAA ACCACATATT CAGTGACTGT AGCCGCGTAC 
ACCACTAAAG GAGATGGAGC GCGGAGCAAA GCCAAAGTGG TGACCACAAC AGGAGCAGTT 
CCAGGAAAGC CCACTATGAT CATCAGCACC ACCATAGGCA ACACGGCTCT GATCCAGTGG 
CAGCCGCCTA AAGAGATGGT GGGAGAGCTG ATGGGCTACA GACTCCGCTA TAAGCGTGAA 
GAAGAGGATA TTTACACTGT CCGAGACTTC AGACGCACTG ATGACCACTT CACTGTCACC 
GGCCTGCACA AGGGCGCGAC GTACATCTTC AAACTCTGCG CTAAAAACCG CGCCGGGAAT 
GGAGAGGAGT ACAGCAAAGA GATCAGCACC CCGGAGGATG TGCCCAGCAG TTACCCACAA 
AACCTCAAAG TCGTTGGATT GACCACAACC ACCACAAAAC TAGCCTGGGA TCCTCCGCCG 
CTGCCCGAGA GGAATGGGAA GATCGTACGA TATGTGGTGG TTTATCGAGA CATTAACAGC 
CATCAGAATC AGACCAACGG CACAGCGGAC ACGGAGATGA CCATTCAGGG CCTGAAGCCG 
GACACCACCT ATGATATCAG GGTGAGGGCG TTTACAGGGA AAGGTGGAGG TCCCATCAGC 
CCCAGCATCC AGAGCAGAAC CATGTCTACC TCCATGCCTG AGTTCACGAA AAACTTTGGT 
GTGAAAGCCG TGATGAAGAC CTCTGTCCTG CTCACATGGG AGGTGCCAGA AACATACAAG 
TCTCAAGTGC CTTTCAAGAT CCTGTATAAC CAGCAGAGTG TGGAGGTCCA GGGTGACCTG 
AAGAGGAAGC TGATTACGCG TCTGCAGCCG GACACTGATT ACTCCTTTGT CCTGATGAGT 
CGGGGGAACA GCGCTGGCGG ACTGCAGCAG CAGGTCTCCA TCCGCACCGC TCCAGACCTG 
TTCACTACCA AACCAGCACA CCAGAAACTA GACCCAGAGG AGGGCGGCAA ACTCGCCATC 
ACACTGCCCG AAGTGCACAA CAGCGCCCTC GTCAGGTGGT TTTATATCGT GGTTGTACCA 
GTAACTCCTT CAACTCTCCG CCATTGGGAA AACCCAGAAG ATATTGATCT GGATGAGCTG 
ATGGGGAATA ATAGAGACGC AGCTGTGAGG AGGAGGAGAA GACAAACTCA GGACTTTCTG 
CGGCCTTACA TTGCTGCCAA GCTGGAGTCT CTTCCAGAAA GATTTGTGTT GGGAGATGAG 
AAAACCTACA GCAACTACTA CAACAAACCT CTGCCGGGAC AGCAGCAGTA TCGCACATTT 
ATACTGGCGG CACTGAAAGA CAGAGACTCG ACGTTCACAG TGAGTCCGTT CTCTGAGCCC 
ATGATGGTGA AGCTCCACAG CGGGATGTCC CGTCACGTGG AGGACCCGGA GATGCTGTGG 
GTGATGGGAC CAGTGTTAGC CGTGGTCCTC ATCATCATCA TCGTCATCGC CATCCTCCTC 
TTCAAGAGCA AACAGGAGAG GAAGCGCACG TCTCCCTCAG CTAAAGATGA GCACTCAGCT 
GGAGTTAAAG ATCACCTGCT GGCTCATTCG TCAGATCCAG TGGAGATGAG GAGGCTCAAC 
TACCAGACAC AAGGAATGCG GGAACACCCT CCAGTGTCTG TGTGTGCTTT GGCTGACCAC 
ATCGAGCGGC TGAGAGCCAA CGACAGCCTC CGCTTCTCGC AGGAGTATGA GTCTGTTGAC 
CCTGGCCAGC AGTTCACCTG GGAAAACTCC AACTTAGAGG TCAACAAGCC CAAGAACCGC 
TACGCAAACG TCATCGCTTA CGATCACTCC AGGGTTGTGC TGACTCCTGT GGATGGTGTT 
CCCGGAAGTG ACTACATCAA TGGAAACTAC ATTGATGGCT ACCGTAAGCA GAACGCCTAC 
ATCGCCACGC AGGGTCCGCT GCCTGAGACG CTCAGTGACT TCTGGAGGAT GGTGTGGGAA 
CAGAGAACCA GCACTATCGT CATGATGACC CGACTGGAGG AGAAGAGCAG GGTGAAGTGT 
GATCAGTACT GGCCAAGCCG AGGAACAGAG ACTTACGGGA TGATTCAAGT GTCCATGCTG 
GATACGGTGG AGCTGGCCAC ATACAGCGTC AGAACCTTCG CTCTCTACAA GAATGGATCC 
AGCGAGAAAC GGGAGGTTCG GCAGTTCCAG TTCATGGCGT GGCCGGATCA CGGTGTTCCC 
GAATATCCCA CACCCATTTT AGCCTTCTTA CGCAGGGTCA AAGCCTGCAA CCCACCTGAC 
GCGGGACCCA TGGTGGTGCA CTGCAGCGCT GGCGTGGGTC GGACGGGCTG TTTCATCGTA 
ATAGACGCCA TGCTGGAGCG GATGAAGCAC GAGAAGTCGG TGGACATCTA CGGTCACGTG 
ACCTGCATGC GTTCTCAGAG GAACTACATG GTGCAGACAG AGGATCAGTA CATTTTCATT 
CACGAGGCCC TGCTGGAGGC TGCCACCTGC GGGAACACAG AGGTGCCGGC GCGGAGCCTC 
TACACACACA TCCAGAAACT CACACAGGCG CCGCCTGGAG ACACCGTCAC CGCCATGGAG 
CTCGAATTTA AGAAACTGGC CAACTCTAAA GCACACACCT CCAGATTCAT CAGCGCTAGT 
CTGCCCTGCA ACAAATTCAA GAACCGGCTG GTGAACATCA TGCCTTTCGA GTCCAGCCGT 
GTTTGTCTGC AGCCAATCAG AGGCGTGGAG GGCTCCGACT ACATCAACGC CAGCTTCATC 
GACGGATACA GGCAGCAGAA GGCATATATC GCCACTCAAG GCCCGCTGTC TGAAACCACA 
GAGGACTTCT GGAGGATGCT TTGGGAGCAC AACTCCACCA TCGTGGTGAT GCTGACGAAG 
CTTAGAGAGA TGGGACGGGA GAAATGTCAT CAGTACTGGC CTGCAGAGAG ATCTGCGCGC 
TACCAGTATT TCGTTGTGGA TCCTATGGCT GAATATAACA TGCCACAGTA CATCTTACGG 
GAGTTTAAAG TCACAGACGC CAGAGATGGC CAGTCTCGGA CTATCCGACA GTTTCAGTTC 
ACAGATTGGC CGGAACAAGG TGTGCCAAAG ACAGGAGAGG GCTTTATTGA TTTTATTGGT 
CAAGTCCACA AAACCAAGGA GCAGTTTGGA CAGGACGGAC CAATTACTGT TCACTGCAGT 
GCCGGTGTGG GCAGAACAGG CGTCTTCATC ACTTTAAGTA TTGTTCTGGA GCGGATGAGG 
TATGAAGGAG TGGTGGACAT GTTCCAGACC ATAAAAACAC TCCGCACGCA GAGACCTGCC 
ATGGTACAGA CCGAGGACCA GTACCAGCTG TGCTACAGAG CAGCTCTGGA GTACCTGGGC 
AGCTTCGATC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfa ( XM_021476767.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332442.1 Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X8, mRNA.

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CloneID	ODa142908
Clone ID Related Accession (Same CDS sequence)	XM_021476767.1
Accession Version	XM_021476767.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGACC AGCGGGAAGT CCGACGGGTT 
CCCCCACGTT TTTCAATCCC TCCAACCAAT CACGAGGTGA TGCCGGGTGG CAGTGTGAAC 
CTCACCTGCG TGGCAGTGGG CGCTCCAATG CCCTATGTGA AGTGGATGAA GGGCGAACAG 
GAGCTGACCA AAGAAGAGGA GATGCCCATC GGCCGAAATG TGCTGGAGCT CACCAACATC 
CGCCAGTCGG GGAACTACAC CTGCGTGGCC ATATCATCAC TGGGCATGAT CGACACCACA 
GCGCAGATCA CTGTTAAAGC TCTTCCTCGT CCTCCAGCCT CTCTGATTGT CACTGAAACC 
ACCGCCACCA GTGTGACTTT AACATGGGAT TCTGGCAACC CAGAACCCGT ATCATATTAC 
GTGATCCAGT ATCGATCCAA AATCTCTGAC AATGGTTTCC AGGAGGTGGA CGGCGTGGCA 
TCCACCCGCT ACAGCATCGG CGGGTTGAGT CCGTACTCAG AGTATGAGTT TCGAGTAATG 
GCGGTCAATA ACATTGGTCG CGGGCCGCCC AGCGGGATGG TGGAGACTCG TACCAGTGAA 
CAGGCACCGT CATCTCCTCC GCTGCGCGTT CAAGCCCGGA TGTTGAGCGC TACGACTATG 
CTGGTGCAAT GGGAACCTCC TGAAGAGCCC AACGGACAGA TTCGAGGATA TCGAGTGTAC 
TACACGTCTG ACCTGCACTT CCCGCTCAGC ACATGGCAGA AGCACAACAC TGAGGACAGC 
AGTCTGACCA CCATCTCCGG CCTGGTTCCT GACATCACGT ATGGGCTCCG TGTGCTGGCC 
TTCACCTCTG TTGGAGATGG ACCACCTTCC GATATACTGC AGGTTAAAAC ACAGCAAGGA 
GTCCCTGCTC AGCCGACTGG TTTTGAAGCT GAAGCCGAGT TGGACACGCG GATCATGCTG 
TCATGGTTGT GGCCCGTCCA AGATCAGATC ACCAAATATG AGCTCACATA CTGGGAGGCT 
GACTCTGACA ACAAGCATCA TGTCATCTTT GACCCGGCCG GTTCATATGC TATTGACAGC 
CTAAAGCCAG ACACTCTCTA TAAATTCTCT CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG 
GTTTACACAC AGCCTATCGA GGCCCGCACA GCCCAGTCCG CCCCGTCCGC TCCCCCACAA 
GAGGTGCACC TGATCAGCCT GAGCTCCAGC AGCATCAAGG TGAGTTGGGT GGCCCCTCCG 
GCCGCCAGCC GGCACGGCAG CATCGTCCGC TACTCTCTGG CCTATCAGGC AGTGTCTGGC 
GAGGACCAGG AGCGCCACGA GGTCAAAGAC ATCCCTGCTG ATGTGTCCAG CTATGTTCTG 
GAGGGTCTGG AGAAATGGAC TGAGTATACG GTGTGGGTCA AAGCGCACAC AGATGTGGGT 
CCCGGGCCAG AAAGTGGCAG CACCCGCATC AGAACCCAAG AGGATGTGCC TGGTGCTCCT 
CCACGCAGGG TTGAGGTGGA CAACATCAAC TCCACTGCAC TGCGTGTGTC CTGGAAGCCG 
CCTCTGCAGC AGAAACAACA CGGCCACATA CGTGGATATC AGGTGGTTTA CTCACGCCTG 
GAGAATGGAG AGCCGCGCGG ACAGCCCATC ATTCTGGACG TGGCGCTGCC AGAGGCACAG 
GAGGCCGTGA TCAGTGGTCT TCTCCCTGAA ACCACATATT CAGTGACTGT AGCCGCGTAC 
ACCACTAAAG GAGATGGAGC GCGGAGCAAA GCCAAAGTGG TGACCACAAC AGGAGCAGTT 
CCAGGAAAGC CCACTATGAT CATCAGCACC ACCATAGGCA ACACGGCTCT GATCCAGTGG 
CAGCCGCCTA AAGAGATGGT GGGAGAGCTG ATGGGCTACA GACTCCGCTA TAAGCGTGAA 
GAAGAGGATA TTTACACTGT CCGAGACTTC AGACGCACTG ATGACCACTT CACTGTCACC 
GGCCTGCACA AGGGCGCGAC GTACATCTTC AAACTCTGCG CTAAAAACCG CGCCGGGAAT 
GGAGAGGAGT ACAGCAAAGA GATCAGCACC CCGGAGGATG TGCCCAGCAG TTACCCACAA 
AACCTCAAAG TCGTTGGATT GACCACAACC ACCACAAAAC TAGCCTGGGA TCCTCCGCCG 
CTGCCCGAGA GGAATGGGAA GATCGTACGA TATGTGGTGG TTTATCGAGA CATTAACAGC 
CATCAGAATC AGACCAACGG CACAGCGGAC ACGGAGATGA CCATTCAGGG CCTGAAGCCG 
GACACCACCT ATGATATCAG GGTGAGGGCG TTTACAGGGA AAGGTGGAGG TCCCATCAGC 
CCCAGCATCC AGAGCAGAAC CATGTCTACC TCCATGCCTG AGTTCACGAA AAACTTTGGT 
GTGAAAGCCG TGATGAAGAC CTCTGTCCTG CTCACATGGG AGGTGCCAGA AACATACAAG 
TCTCAAGTGC CTTTCAAGAT CCTGTATAAC CAGCAGAGTG TGGAGGTCCA GGGTGACCTG 
AAGAGGAAGC TGATTACGCG TCTGCAGCCG GACACTGATT ACTCCTTTGT CCTGATGAGT 
CGGGGGAACA GCGCTGGCGG ACTGCAGCAG CAGGTCTCCA TCCGCACCGC TCCAGACCTG 
TTCACTACCA AACCAGCACA CCAGAAACTA GACCCAGAGG AGGGCGGCAA ACTCGCCATC 
ACACTGCCCG AAGTGCACAA CAGCGCCCTC GTCAGGTGGT TTTATATCGT GGTTGTACCA 
GTAACTCCTT CAACTCTCCG CCATTGGGAA AACCCAGAAG ATATTGATCT GGATGAGCTG 
ATGGGGAATA ATAGAGACGC AGCTGTGAGG AGGAGGAGAA GACAAACTCA GGACTTTCTG 
CGGCCTTACA TTGCTGCCAA GCTGGAGTCT CTTCCAGAAA GATTTGTGTT GGGAGATGAG 
AAAACCTACA GCAACTACTA CAACAAACCT CTGCCGGGAC AGCAGCAGTA TCGCACATTT 
ATACTGGCGG CACTGAAAGA CAGAGACTCG ACGTTCACAG TGAGTCCGTT CTCTGAGCCC 
ATGATGGTGA AGCTCCACAG CGGGATGTCC CGTCACGTGG AGGACCCGGA GATGCTGTGG 
GTGATGGGAC CAGTGTTAGC CGTGGTCCTC ATCATCATCA TCGTCATCGC CATCCTCCTC 
TTCAAGAGGA AGCGCACGTC TCCCTCAGCT AAAGATGAGC ACTCAGCTGG AGTTAAAGAT 
CACCTGCTGG CTCATTCGTC AGATCCAGTG GAGATGAGGA GGCTCAACTA CCAGACACAA 
GGAATGCGGG AACACCCTCC AGTGTCTGTG TGTGCTTTGG CTGACCACAT CGAGCGGCTG 
AGAGCCAACG ACAGCCTCCG CTTCTCGCAG GAGTATGAGT CTGTTGACCC TGGCCAGCAG 
TTCACCTGGG AAAACTCCAA CTTAGAGGTC AACAAGCCCA AGAACCGCTA CGCAAACGTC 
ATCGCTTACG ATCACTCCAG GGTTGTGCTG ACTCCTGTGG ATGGTGTTCC CGGAAGTGAC 
TACATCAATG GAAACTACAT TGATGGCTAC CGTAAGCAGA ACGCCTACAT CGCCACGCAG 
GGTCCGCTGC CTGAGACGCT CAGTGACTTC TGGAGGATGG TGTGGGAACA GAGAACCAGC 
ACTATCGTCA TGATGACCCG ACTGGAGGAG AAGAGCAGGG TGAAGTGTGA TCAGTACTGG 
CCAAGCCGAG GAACAGAGAC TTACGGGATG ATTCAAGTGT CCATGCTGGA TACGGTGGAG 
CTGGCCACAT ACAGCGTCAG AACCTTCGCT CTCTACAAGA ATGGATCCAG CGAGAAACGG 
GAGGTTCGGC AGTTCCAGTT CATGGCGTGG CCGGATCACG GTGTTCCCGA ATATCCCACA 
CCCATTTTAG CCTTCTTACG CAGGGTCAAA GCCTGCAACC CACCTGACGC GGGACCCATG 
GTGGTGCACT GCAGCGCTGG CGTGGGTCGG ACGGGCTGTT TCATCGTAAT AGACGCCATG 
CTGGAGCGGA TGAAGCACGA GAAGTCGGTG GACATCTACG GTCACGTGAC CTGCATGCGT 
TCTCAGAGGA ACTACATGGT GCAGACAGAG GATCAGTACA TTTTCATTCA CGAGGCCCTG 
CTGGAGGCTG CCACCTGCGG GAACACAGAG GTGCCGGCGC GGAGCCTCTA CACACACATC 
CAGAAACTCA CACAGGCGCC GCCTGGAGAC ACCGTCACCG CCATGGAGCT CGAATTTAAG 
AAACTGGCCA ACTCTAAAGC ACACACCTCC AGATTCATCA GCGCTAGTCT GCCCTGCAAC 
AAATTCAAGA ACCGGCTGGT GAACATCATG CCTTTCGAGT CCAGCCGTGT TTGTCTGCAG 
CCAATCAGAG GCGTGGAGGG CTCCGACTAC ATCAACGCCA GCTTCATCGA CGGATACAGG 
CAGCAGAAGG CATATATCGC CACTCAAGGC CCGCTGTCTG AAACCACAGA GGACTTCTGG 
AGGATGCTTT GGGAGCACAA CTCCACCATC GTGGTGATGC TGACGAAGCT TAGAGAGATG 
GGACGGGAGA AATGTCATCA GTACTGGCCT GCAGAGAGAT CTGCGCGCTA CCAGTATTTC 
GTTGTGGATC CTATGGCTGA ATATAACATG CCACAGTACA TCTTACGGGA GTTTAAAGTC 
ACAGACGCCA GAGATGGCCA GTCTCGGACT ATCCGACAGT TTCAGTTCAC AGATTGGCCG 
GAACAAGGTG TGCCAAAGAC AGGAGAGGGC TTTATTGATT TTATTGGTCA AGTCCACAAA 
ACCAAGGAGC AGTTTGGACA GGACGGACCA ATTACTGTTC ACTGCAGTGC CGGTGTGGGC 
AGAACAGGCG TCTTCATCAC TTTAAGTATT GTTCTGGAGC GGATGAGGTA TGAAGGAGTG 
GTGGACATGT TCCAGACCAT AAAAACACTC CGCACGCAGA GACCTGCCAT GGTACAGACC 
GAGGACCAGT ACCAGCTGTG CTACAGAGCA GCTCTGGAGT ACCTGGGCAG CTTCGATCAC 
TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332442.1
CDS	1458..7169
Translation	MVPQTRQGSARHGATSLLLLLCLAVPIHADSMPSFVKTPEDQTGISGGVASFVCQAVGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEEDQIPHGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEISWFKDFLPVDINSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAVNSAGTRYSAPANLYVRDQREVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMKGEQELTKEEEMPIGRNVLELTNIRQSGNYTCVAISSLGMIDTTAQITVKALPRPPASLIVTETTATSVTLTWDSGNPEPVSYYVIQYRSKISDNGFQEVDGVASTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSGMVETRTSEQAPSSPPLRVQARMLSATTMLVQWEPPEEPNGQIRGYRVYYTSDLHFPLSTWQKHNTEDSSLTTISGLVPDITYGLRVLAFTSVGDGPPSDILQVKTQQGVPAQPTGFEAEAELDTRIMLSWLWPVQDQITKYELTYWEADSDNKHHVIFDPAGSYAIDSLKPDTLYKFSLAARSEMGLGVYTQPIEARTAQSAPSAPPQEVHLISLSSSSIKVSWVAPPAASRHGSIVRYSLAYQAVSGEDQERHEVKDIPADVSSYVLEGLEKWTEYTVWVKAHTDVGPGPESGSTRIRTQEDVPGAPPRRVEVDNINSTALRVSWKPPLQQKQHGHIRGYQVVYSRLENGEPRGQPIILDVALPEAQEAVISGLLPETTYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTIGNTALIQWQPPKEMVGELMGYRLRYKREEEDIYTVRDFRRTDDHFTVTGLHKGATYIFKLCAKNRAGNGEEYSKEISTPEDVPSSYPQNLKVVGLTTTTTKLAWDPPPLPERNGKIVRYVVVYRDINSHQNQTNGTADTEMTIQGLKPDTTYDIRVRAFTGKGGGPISPSIQSRTMSTSMPEFTKNFGVKAVMKTSVLLTWEVPETYKSQVPFKILYNQQSVEVQGDLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFTTKPAHQKLDPEEGGKLAITLPEVHNSALVRWFYIVVVPVTPSTLRHWENPEDIDLDELMGNNRDAAVRRRRRQTQDFLRPYIAAKLESLPERFVLGDEKTYSNYYNKPLPGQQQYRTFILAALKDRDSTFTVSPFSEPMMVKLHSGMSRHVEDPEMLWVMGPVLAVVLIIIIVIAILLFKRKRTSPSAKDEHSAGVKDHLLAHSSDPVEMRRLNYQTQGMREHPPVSVCALADHIERLRANDSLRFSQEYESVDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINGNYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRTSTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVSMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARSLYTHIQKLTQAPPGDTVTAMELEFKKLANSKAHTSRFISASLPCNKFKNRLVNIMPFESSRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLSETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTIKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021476767.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476767.1

ORF Insert Sequence:

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGACC AGCGGGAAGT CCGACGGGTT 
CCCCCACGTT TTTCAATCCC TCCAACCAAT CACGAGGTGA TGCCGGGTGG CAGTGTGAAC 
CTCACCTGCG TGGCAGTGGG CGCTCCAATG CCCTATGTGA AGTGGATGAA GGGCGAACAG 
GAGCTGACCA AAGAAGAGGA GATGCCCATC GGCCGAAATG TGCTGGAGCT CACCAACATC 
CGCCAGTCGG GGAACTACAC CTGCGTGGCC ATATCATCAC TGGGCATGAT CGACACCACA 
GCGCAGATCA CTGTTAAAGC TCTTCCTCGT CCTCCAGCCT CTCTGATTGT CACTGAAACC 
ACCGCCACCA GTGTGACTTT AACATGGGAT TCTGGCAACC CAGAACCCGT ATCATATTAC 
GTGATCCAGT ATCGATCCAA AATCTCTGAC AATGGTTTCC AGGAGGTGGA CGGCGTGGCA 
TCCACCCGCT ACAGCATCGG CGGGTTGAGT CCGTACTCAG AGTATGAGTT TCGAGTAATG 
GCGGTCAATA ACATTGGTCG CGGGCCGCCC AGCGGGATGG TGGAGACTCG TACCAGTGAA 
CAGGCACCGT CATCTCCTCC GCTGCGCGTT CAAGCCCGGA TGTTGAGCGC TACGACTATG 
CTGGTGCAAT GGGAACCTCC TGAAGAGCCC AACGGACAGA TTCGAGGATA TCGAGTGTAC 
TACACGTCTG ACCTGCACTT CCCGCTCAGC ACATGGCAGA AGCACAACAC TGAGGACAGC 
AGTCTGACCA CCATCTCCGG CCTGGTTCCT GACATCACGT ATGGGCTCCG TGTGCTGGCC 
TTCACCTCTG TTGGAGATGG ACCACCTTCC GATATACTGC AGGTTAAAAC ACAGCAAGGA 
GTCCCTGCTC AGCCGACTGG TTTTGAAGCT GAAGCCGAGT TGGACACGCG GATCATGCTG 
TCATGGTTGT GGCCCGTCCA AGATCAGATC ACCAAATATG AGCTCACATA CTGGGAGGCT 
GACTCTGACA ACAAGCATCA TGTCATCTTT GACCCGGCCG GTTCATATGC TATTGACAGC 
CTAAAGCCAG ACACTCTCTA TAAATTCTCT CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG 
GTTTACACAC AGCCTATCGA GGCCCGCACA GCCCAGTCCG CCCCGTCCGC TCCCCCACAA 
GAGGTGCACC TGATCAGCCT GAGCTCCAGC AGCATCAAGG TGAGTTGGGT GGCCCCTCCG 
GCCGCCAGCC GGCACGGCAG CATCGTCCGC TACTCTCTGG CCTATCAGGC AGTGTCTGGC 
GAGGACCAGG AGCGCCACGA GGTCAAAGAC ATCCCTGCTG ATGTGTCCAG CTATGTTCTG 
GAGGGTCTGG AGAAATGGAC TGAGTATACG GTGTGGGTCA AAGCGCACAC AGATGTGGGT 
CCCGGGCCAG AAAGTGGCAG CACCCGCATC AGAACCCAAG AGGATGTGCC TGGTGCTCCT 
CCACGCAGGG TTGAGGTGGA CAACATCAAC TCCACTGCAC TGCGTGTGTC CTGGAAGCCG 
CCTCTGCAGC AGAAACAACA CGGCCACATA CGTGGATATC AGGTGGTTTA CTCACGCCTG 
GAGAATGGAG AGCCGCGCGG ACAGCCCATC ATTCTGGACG TGGCGCTGCC AGAGGCACAG 
GAGGCCGTGA TCAGTGGTCT TCTCCCTGAA ACCACATATT CAGTGACTGT AGCCGCGTAC 
ACCACTAAAG GAGATGGAGC GCGGAGCAAA GCCAAAGTGG TGACCACAAC AGGAGCAGTT 
CCAGGAAAGC CCACTATGAT CATCAGCACC ACCATAGGCA ACACGGCTCT GATCCAGTGG 
CAGCCGCCTA AAGAGATGGT GGGAGAGCTG ATGGGCTACA GACTCCGCTA TAAGCGTGAA 
GAAGAGGATA TTTACACTGT CCGAGACTTC AGACGCACTG ATGACCACTT CACTGTCACC 
GGCCTGCACA AGGGCGCGAC GTACATCTTC AAACTCTGCG CTAAAAACCG CGCCGGGAAT 
GGAGAGGAGT ACAGCAAAGA GATCAGCACC CCGGAGGATG TGCCCAGCAG TTACCCACAA 
AACCTCAAAG TCGTTGGATT GACCACAACC ACCACAAAAC TAGCCTGGGA TCCTCCGCCG 
CTGCCCGAGA GGAATGGGAA GATCGTACGA TATGTGGTGG TTTATCGAGA CATTAACAGC 
CATCAGAATC AGACCAACGG CACAGCGGAC ACGGAGATGA CCATTCAGGG CCTGAAGCCG 
GACACCACCT ATGATATCAG GGTGAGGGCG TTTACAGGGA AAGGTGGAGG TCCCATCAGC 
CCCAGCATCC AGAGCAGAAC CATGTCTACC TCCATGCCTG AGTTCACGAA AAACTTTGGT 
GTGAAAGCCG TGATGAAGAC CTCTGTCCTG CTCACATGGG AGGTGCCAGA AACATACAAG 
TCTCAAGTGC CTTTCAAGAT CCTGTATAAC CAGCAGAGTG TGGAGGTCCA GGGTGACCTG 
AAGAGGAAGC TGATTACGCG TCTGCAGCCG GACACTGATT ACTCCTTTGT CCTGATGAGT 
CGGGGGAACA GCGCTGGCGG ACTGCAGCAG CAGGTCTCCA TCCGCACCGC TCCAGACCTG 
TTCACTACCA AACCAGCACA CCAGAAACTA GACCCAGAGG AGGGCGGCAA ACTCGCCATC 
ACACTGCCCG AAGTGCACAA CAGCGCCCTC GTCAGGTGGT TTTATATCGT GGTTGTACCA 
GTAACTCCTT CAACTCTCCG CCATTGGGAA AACCCAGAAG ATATTGATCT GGATGAGCTG 
ATGGGGAATA ATAGAGACGC AGCTGTGAGG AGGAGGAGAA GACAAACTCA GGACTTTCTG 
CGGCCTTACA TTGCTGCCAA GCTGGAGTCT CTTCCAGAAA GATTTGTGTT GGGAGATGAG 
AAAACCTACA GCAACTACTA CAACAAACCT CTGCCGGGAC AGCAGCAGTA TCGCACATTT 
ATACTGGCGG CACTGAAAGA CAGAGACTCG ACGTTCACAG TGAGTCCGTT CTCTGAGCCC 
ATGATGGTGA AGCTCCACAG CGGGATGTCC CGTCACGTGG AGGACCCGGA GATGCTGTGG 
GTGATGGGAC CAGTGTTAGC CGTGGTCCTC ATCATCATCA TCGTCATCGC CATCCTCCTC 
TTCAAGAGGA AGCGCACGTC TCCCTCAGCT AAAGATGAGC ACTCAGCTGG AGTTAAAGAT 
CACCTGCTGG CTCATTCGTC AGATCCAGTG GAGATGAGGA GGCTCAACTA CCAGACACAA 
GGAATGCGGG AACACCCTCC AGTGTCTGTG TGTGCTTTGG CTGACCACAT CGAGCGGCTG 
AGAGCCAACG ACAGCCTCCG CTTCTCGCAG GAGTATGAGT CTGTTGACCC TGGCCAGCAG 
TTCACCTGGG AAAACTCCAA CTTAGAGGTC AACAAGCCCA AGAACCGCTA CGCAAACGTC 
ATCGCTTACG ATCACTCCAG GGTTGTGCTG ACTCCTGTGG ATGGTGTTCC CGGAAGTGAC 
TACATCAATG GAAACTACAT TGATGGCTAC CGTAAGCAGA ACGCCTACAT CGCCACGCAG 
GGTCCGCTGC CTGAGACGCT CAGTGACTTC TGGAGGATGG TGTGGGAACA GAGAACCAGC 
ACTATCGTCA TGATGACCCG ACTGGAGGAG AAGAGCAGGG TGAAGTGTGA TCAGTACTGG 
CCAAGCCGAG GAACAGAGAC TTACGGGATG ATTCAAGTGT CCATGCTGGA TACGGTGGAG 
CTGGCCACAT ACAGCGTCAG AACCTTCGCT CTCTACAAGA ATGGATCCAG CGAGAAACGG 
GAGGTTCGGC AGTTCCAGTT CATGGCGTGG CCGGATCACG GTGTTCCCGA ATATCCCACA 
CCCATTTTAG CCTTCTTACG CAGGGTCAAA GCCTGCAACC CACCTGACGC GGGACCCATG 
GTGGTGCACT GCAGCGCTGG CGTGGGTCGG ACGGGCTGTT TCATCGTAAT AGACGCCATG 
CTGGAGCGGA TGAAGCACGA GAAGTCGGTG GACATCTACG GTCACGTGAC CTGCATGCGT 
TCTCAGAGGA ACTACATGGT GCAGACAGAG GATCAGTACA TTTTCATTCA CGAGGCCCTG 
CTGGAGGCTG CCACCTGCGG GAACACAGAG GTGCCGGCGC GGAGCCTCTA CACACACATC 
CAGAAACTCA CACAGGCGCC GCCTGGAGAC ACCGTCACCG CCATGGAGCT CGAATTTAAG 
AAACTGGCCA ACTCTAAAGC ACACACCTCC AGATTCATCA GCGCTAGTCT GCCCTGCAAC 
AAATTCAAGA ACCGGCTGGT GAACATCATG CCTTTCGAGT CCAGCCGTGT TTGTCTGCAG 
CCAATCAGAG GCGTGGAGGG CTCCGACTAC ATCAACGCCA GCTTCATCGA CGGATACAGG 
CAGCAGAAGG CATATATCGC CACTCAAGGC CCGCTGTCTG AAACCACAGA GGACTTCTGG 
AGGATGCTTT GGGAGCACAA CTCCACCATC GTGGTGATGC TGACGAAGCT TAGAGAGATG 
GGACGGGAGA AATGTCATCA GTACTGGCCT GCAGAGAGAT CTGCGCGCTA CCAGTATTTC 
GTTGTGGATC CTATGGCTGA ATATAACATG CCACAGTACA TCTTACGGGA GTTTAAAGTC 
ACAGACGCCA GAGATGGCCA GTCTCGGACT ATCCGACAGT TTCAGTTCAC AGATTGGCCG 
GAACAAGGTG TGCCAAAGAC AGGAGAGGGC TTTATTGATT TTATTGGTCA AGTCCACAAA 
ACCAAGGAGC AGTTTGGACA GGACGGACCA ATTACTGTTC ACTGCAGTGC CGGTGTGGGC 
AGAACAGGCG TCTTCATCAC TTTAAGTATT GTTCTGGAGC GGATGAGGTA TGAAGGAGTG 
GTGGACATGT TCCAGACCAT AAAAACACTC CGCACGCAGA GACCTGCCAT GGTACAGACC 
GAGGACCAGT ACCAGCTGTG CTACAGAGCA GCTCTGGAGT ACCTGGGCAG CTTCGATCAC 
TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfa ( XM_021476768.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332443.1 Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X9, mRNA.

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CloneID	ODa142909
Clone ID Related Accession (Same CDS sequence)	XM_021476768.1
Accession Version	XM_021476768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGACC AGCGGGAAGT CCGACGGGTT 
CCCCCACGTT TTTCAATCCC TCCAACCAAT CACGAGGTGA TGCCGGGTGG CAGTGTGAAC 
CTCACCTGCG TGGCAGTGGG CGCTCCAATG CCCTATGTGA AGTGGATGAA GGGCGAACAG 
GAGCTGACCA AAGAAGAGGA GATGCCCATC GGCCGAAATG TGCTGGAGCT CACCAACATC 
CGCCAGTCGG GGAACTACAC CTGCGTGGCC ATATCATCAC TGGGCATGAT CGACACCACA 
GCGCAGATCA CTGTTAAAGC TCTTCCTCGT CCTCCAGCCT CTCTGATTGT CACTGAAACC 
ACCGCCACCA GTGTGACTTT AACATGGGAT TCTGGCAACC CAGAACCCGT ATCATATTAC 
GTGATCCAGT ATCGATCCAA AATCTCTGAC AATGGTTTCC AGGAGGTGGA CGGCGTGGCA 
TCCACCCGCT ACAGCATCGG CGGGTTGAGT CCGTACTCAG AGTATGAGTT TCGAGTAATG 
GCGGTCAATA ACATTGGTCG CGGGCCGCCC AGCGGGATGG TGGAGACTCG TACCAGTGAA 
CAGGCACCGT CATCTCCTCC GCTGCGCGTT CAAGCCCGGA TGTTGAGCGC TACGACTATG 
CTGGTGCAAT GGGAACCTCC TGAAGAGCCC AACGGACAGA TTCGAGGATA TCGAGTGTAC 
TACACGTCTG ACCTGCACTT CCCGCTCAGC ACATGGCAGA AGCACAACAC TGAGGACAGC 
AGTCTGACCA CCATCTCCGG CCTGGTTCCT GACATCACGT ATGGGCTCCG TGTGCTGGCC 
TTCACCTCTG TTGGAGATGG ACCACCTTCC GATATACTGC AGGTTAAAAC ACAGCAAGGA 
GTCCCTGCTC AGCCGACTGG TTTTGAAGCT GAAGCCGAGT TGGACACGCG GATCATGCTG 
TCATGGTTGT GGCCCGTCCA AGATCAGATC ACCAAATATG AGCTCACATA CTGGGAGGCT 
GACTCTGACA ACAAGCATCA TGTCATCTTT GACCCGGCCG GTTCATATGC TATTGACAGC 
CTAAAGCCAG ACACTCTCTA TAAATTCTCT CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG 
GTTTACACAC AGCCTATCGA GGCCCGCACA GCCCAGTCCG TGCCTGGTGC TCCTCCACGC 
AGGGTTGAGG TGGACAACAT CAACTCCACT GCACTGCGTG TGTCCTGGAA GCCGCCTCTG 
CAGCAGAAAC AACACGGCCA CATACGTGGA TATCAGGTGG TTTACTCACG CCTGGAGAAT 
GGAGAGCCGC GCGGACAGCC CATCATTCTG GACGTGGCGC TGCCAGAGGC ACAGGAGGCC 
GTGATCAGTG GTCTTCTCCC TGAAACCACA TATTCAGTGA CTGTAGCCGC GTACACCACT 
AAAGGAGATG GAGCGCGGAG CAAAGCCAAA GTGGTGACCA CAACAGGAGC AGTTCCAGGA 
AAGCCCACTA TGATCATCAG CACCACCATA GGCAACACGG CTCTGATCCA GTGGCAGCCG 
CCTAAAGAGA TGGTGGGAGA GCTGATGGGC TACAGACTCC GCTATAAGCG TGAAGAAGAG 
GATATTTACA CTGTCCGAGA CTTCAGACGC ACTGATGACC ACTTCACTGT CACCGGCCTG 
CACAAGGGCG CGACGTACAT CTTCAAACTC TGCGCTAAAA ACCGCGCCGG GAATGGAGAG 
GAGTACAGCA AAGAGATCAG CACCCCGGAG GATGTGCCCA GCAGTTACCC ACAAAACCTC 
AAAGTCGTTG GATTGACCAC AACCACCACA AAACTAGCCT GGGATCCTCC GCCGCTGCCC 
GAGAGGAATG GGAAGATCGT ACGATATGTG GTGGTTTATC GAGACATTAA CAGCCATCAG 
AATCAGACCA ACGGCACAGC GGACACGGAG ATGACCATTC AGGGCCTGAA GCCGGACACC 
ACCTATGATA TCAGGGTGAG GGCGTTTACA GGGAAAGGTG GAGGTCCCAT CAGCCCCAGC 
ATCCAGAGCA GAACCATGTC TACCTCCATG CCTGAGTTCA CGAAAAACTT TGGTGTGAAA 
GCCGTGATGA AGACCTCTGT CCTGCTCACA TGGGAGGTGC CAGAAACATA CAAGTCTCAA 
GTGCCTTTCA AGATCCTGTA TAACCAGCAG AGTGTGGAGG TCCAGGGTGA CCTGAAGAGG 
AAGCTGATTA CGCGTCTGCA GCCGGACACT GATTACTCCT TTGTCCTGAT GAGTCGGGGG 
AACAGCGCTG GCGGACTGCA GCAGCAGGTC TCCATCCGCA CCGCTCCAGA CCTGTTCACT 
ACCAAACCAG CACACCAGAA ACTAGACCCA GAGGAGGGCG GCAAACTCGC CATCACACTG 
CCCGAAGTGC ACAACAGCGC CCTCGTCAGG TGGTTTTATA TCGTGGTTGT ACCAGTAACT 
CCTTCAACTC TCCGCCATTG GGAAAACCCA GAAGATATTG ATCTGGATGA GCTGATGGGG 
AATAATAGAG ACGCAGCTGT GAGGAGGAGG AGAAGACAAA CTCAGGACTT TCTGCGGCCT 
TACATTGCTG CCAAGCTGGA GTCTCTTCCA GAAAGATTTG TGTTGGGAGA TGAGAAAACC 
TACAGCAACT ACTACAACAA ACCTCTGCCG GGACAGCAGC AGTATCGCAC ATTTATACTG 
GCGGCACTGA AAGACAGAGA CTCGACGTTC ACAGTGAGTC CGTTCTCTGA GCCCATGATG 
GTGAAGCTCC ACAGCGGGAT GTCCCGTCAC GTGGAGGACC CGGAGATGCT GTGGGTGATG 
GGACCAGTGT TAGCCGTGGT CCTCATCATC ATCATCGTCA TCGCCATCCT CCTCTTCAAG 
AGCAAACAGG AGAGGAAGCG CACGTCTCCC TCAGCTAAAG ATGAGCACTC AGCTGGAGTT 
AAAGATCACC TGCTGGCTCA TTCGTCAGAT CCAGTGGAGA TGAGGAGGCT CAACTACCAG 
ACACAAGGTT CCAGTGCCTT CAACTGTCCC AGCACTTCAA GAATGCGGGA ACACCCTCCA 
GTGTCTGTGT GTGCTTTGGC TGACCACATC GAGCGGCTGA GAGCCAACGA CAGCCTCCGC 
TTCTCGCAGG AGTATGAGTC TGTTGACCCT GGCCAGCAGT TCACCTGGGA AAACTCCAAC 
TTAGAGGTCA ACAAGCCCAA GAACCGCTAC GCAAACGTCA TCGCTTACGA TCACTCCAGG 
GTTGTGCTGA CTCCTGTGGA TGGTGTTCCC GGAAGTGACT ACATCAATGG AAACTACATT 
GATGGCTACC GTAAGCAGAA CGCCTACATC GCCACGCAGG GTCCGCTGCC TGAGACGCTC 
AGTGACTTCT GGAGGATGGT GTGGGAACAG AGAACCAGCA CTATCGTCAT GATGACCCGA 
CTGGAGGAGA AGAGCAGGGT GAAGTGTGAT CAGTACTGGC CAAGCCGAGG AACAGAGACT 
TACGGGATGA TTCAAGTGTC CATGCTGGAT ACGGTGGAGC TGGCCACATA CAGCGTCAGA 
ACCTTCGCTC TCTACAAGAA TGGATCCAGC GAGAAACGGG AGGTTCGGCA GTTCCAGTTC 
ATGGCGTGGC CGGATCACGG TGTTCCCGAA TATCCCACAC CCATTTTAGC CTTCTTACGC 
AGGGTCAAAG CCTGCAACCC ACCTGACGCG GGACCCATGG TGGTGCACTG CAGCGCTGGC 
GTGGGTCGGA CGGGCTGTTT CATCGTAATA GACGCCATGC TGGAGCGGAT GAAGCACGAG 
AAGTCGGTGG ACATCTACGG TCACGTGACC TGCATGCGTT CTCAGAGGAA CTACATGGTG 
CAGACAGAGG ATCAGTACAT TTTCATTCAC GAGGCCCTGC TGGAGGCTGC CACCTGCGGG 
AACACAGAGG TGCCGGCGCG GAGCCTCTAC ACACACATCC AGAAACTCAC ACAGGCGCCG 
CCTGGAGACA CCGTCACCGC CATGGAGCTC GAATTTAAGA AACTGGCCAA CTCTAAAGCA 
CACACCTCCA GATTCATCAG CGCTAGTCTG CCCTGCAACA AATTCAAGAA CCGGCTGGTG 
AACATCATGC CTTTCGAGTC CAGCCGTGTT TGTCTGCAGC CAATCAGAGG CGTGGAGGGC 
TCCGACTACA TCAACGCCAG CTTCATCGAC GGATACAGGC AGCAGAAGGC ATATATCGCC 
ACTCAAGGCC CGCTGTCTGA AACCACAGAG GACTTCTGGA GGATGCTTTG GGAGCACAAC 
TCCACCATCG TGGTGATGCT GACGAAGCTT AGAGAGATGG GACGGGAGAA ATGTCATCAG 
TACTGGCCTG CAGAGAGATC TGCGCGCTAC CAGTATTTCG TTGTGGATCC TATGGCTGAA 
TATAACATGC CACAGTACAT CTTACGGGAG TTTAAAGTCA CAGACGCCAG AGATGGCCAG 
TCTCGGACTA TCCGACAGTT TCAGTTCACA GATTGGCCGG AACAAGGTGT GCCAAAGACA 
GGAGAGGGCT TTATTGATTT TATTGGTCAA GTCCACAAAA CCAAGGAGCA GTTTGGACAG 
GACGGACCAA TTACTGTTCA CTGCAGTGCC GGTGTGGGCA GAACAGGCGT CTTCATCACT 
TTAAGTATTG TTCTGGAGCG GATGAGGTAT GAAGGAGTGG TGGACATGTT CCAGACCATA 
AAAACACTCC GCACGCAGAG ACCTGCCATG GTACAGACCG AGGACCAGTA CCAGCTGTGC 
TACAGAGCAG CTCTGGAGTA CCTGGGCAGC TTCGATCACT ATGCAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332443.1
CDS	1458..6908
Translation	MVPQTRQGSARHGATSLLLLLCLAVPIHADSMPSFVKTPEDQTGISGGVASFVCQAVGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEEDQIPHGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEISWFKDFLPVDINSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAVNSAGTRYSAPANLYVRDQREVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMKGEQELTKEEEMPIGRNVLELTNIRQSGNYTCVAISSLGMIDTTAQITVKALPRPPASLIVTETTATSVTLTWDSGNPEPVSYYVIQYRSKISDNGFQEVDGVASTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSGMVETRTSEQAPSSPPLRVQARMLSATTMLVQWEPPEEPNGQIRGYRVYYTSDLHFPLSTWQKHNTEDSSLTTISGLVPDITYGLRVLAFTSVGDGPPSDILQVKTQQGVPAQPTGFEAEAELDTRIMLSWLWPVQDQITKYELTYWEADSDNKHHVIFDPAGSYAIDSLKPDTLYKFSLAARSEMGLGVYTQPIEARTAQSVPGAPPRRVEVDNINSTALRVSWKPPLQQKQHGHIRGYQVVYSRLENGEPRGQPIILDVALPEAQEAVISGLLPETTYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTIGNTALIQWQPPKEMVGELMGYRLRYKREEEDIYTVRDFRRTDDHFTVTGLHKGATYIFKLCAKNRAGNGEEYSKEISTPEDVPSSYPQNLKVVGLTTTTTKLAWDPPPLPERNGKIVRYVVVYRDINSHQNQTNGTADTEMTIQGLKPDTTYDIRVRAFTGKGGGPISPSIQSRTMSTSMPEFTKNFGVKAVMKTSVLLTWEVPETYKSQVPFKILYNQQSVEVQGDLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFTTKPAHQKLDPEEGGKLAITLPEVHNSALVRWFYIVVVPVTPSTLRHWENPEDIDLDELMGNNRDAAVRRRRRQTQDFLRPYIAAKLESLPERFVLGDEKTYSNYYNKPLPGQQQYRTFILAALKDRDSTFTVSPFSEPMMVKLHSGMSRHVEDPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRTSPSAKDEHSAGVKDHLLAHSSDPVEMRRLNYQTQGSSAFNCPSTSRMREHPPVSVCALADHIERLRANDSLRFSQEYESVDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINGNYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRTSTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVSMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARSLYTHIQKLTQAPPGDTVTAMELEFKKLANSKAHTSRFISASLPCNKFKNRLVNIMPFESSRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLSETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTIKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021476768.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476768.1

ORF Insert Sequence:

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGACC AGCGGGAAGT CCGACGGGTT 
CCCCCACGTT TTTCAATCCC TCCAACCAAT CACGAGGTGA TGCCGGGTGG CAGTGTGAAC 
CTCACCTGCG TGGCAGTGGG CGCTCCAATG CCCTATGTGA AGTGGATGAA GGGCGAACAG 
GAGCTGACCA AAGAAGAGGA GATGCCCATC GGCCGAAATG TGCTGGAGCT CACCAACATC 
CGCCAGTCGG GGAACTACAC CTGCGTGGCC ATATCATCAC TGGGCATGAT CGACACCACA 
GCGCAGATCA CTGTTAAAGC TCTTCCTCGT CCTCCAGCCT CTCTGATTGT CACTGAAACC 
ACCGCCACCA GTGTGACTTT AACATGGGAT TCTGGCAACC CAGAACCCGT ATCATATTAC 
GTGATCCAGT ATCGATCCAA AATCTCTGAC AATGGTTTCC AGGAGGTGGA CGGCGTGGCA 
TCCACCCGCT ACAGCATCGG CGGGTTGAGT CCGTACTCAG AGTATGAGTT TCGAGTAATG 
GCGGTCAATA ACATTGGTCG CGGGCCGCCC AGCGGGATGG TGGAGACTCG TACCAGTGAA 
CAGGCACCGT CATCTCCTCC GCTGCGCGTT CAAGCCCGGA TGTTGAGCGC TACGACTATG 
CTGGTGCAAT GGGAACCTCC TGAAGAGCCC AACGGACAGA TTCGAGGATA TCGAGTGTAC 
TACACGTCTG ACCTGCACTT CCCGCTCAGC ACATGGCAGA AGCACAACAC TGAGGACAGC 
AGTCTGACCA CCATCTCCGG CCTGGTTCCT GACATCACGT ATGGGCTCCG TGTGCTGGCC 
TTCACCTCTG TTGGAGATGG ACCACCTTCC GATATACTGC AGGTTAAAAC ACAGCAAGGA 
GTCCCTGCTC AGCCGACTGG TTTTGAAGCT GAAGCCGAGT TGGACACGCG GATCATGCTG 
TCATGGTTGT GGCCCGTCCA AGATCAGATC ACCAAATATG AGCTCACATA CTGGGAGGCT 
GACTCTGACA ACAAGCATCA TGTCATCTTT GACCCGGCCG GTTCATATGC TATTGACAGC 
CTAAAGCCAG ACACTCTCTA TAAATTCTCT CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG 
GTTTACACAC AGCCTATCGA GGCCCGCACA GCCCAGTCCG TGCCTGGTGC TCCTCCACGC 
AGGGTTGAGG TGGACAACAT CAACTCCACT GCACTGCGTG TGTCCTGGAA GCCGCCTCTG 
CAGCAGAAAC AACACGGCCA CATACGTGGA TATCAGGTGG TTTACTCACG CCTGGAGAAT 
GGAGAGCCGC GCGGACAGCC CATCATTCTG GACGTGGCGC TGCCAGAGGC ACAGGAGGCC 
GTGATCAGTG GTCTTCTCCC TGAAACCACA TATTCAGTGA CTGTAGCCGC GTACACCACT 
AAAGGAGATG GAGCGCGGAG CAAAGCCAAA GTGGTGACCA CAACAGGAGC AGTTCCAGGA 
AAGCCCACTA TGATCATCAG CACCACCATA GGCAACACGG CTCTGATCCA GTGGCAGCCG 
CCTAAAGAGA TGGTGGGAGA GCTGATGGGC TACAGACTCC GCTATAAGCG TGAAGAAGAG 
GATATTTACA CTGTCCGAGA CTTCAGACGC ACTGATGACC ACTTCACTGT CACCGGCCTG 
CACAAGGGCG CGACGTACAT CTTCAAACTC TGCGCTAAAA ACCGCGCCGG GAATGGAGAG 
GAGTACAGCA AAGAGATCAG CACCCCGGAG GATGTGCCCA GCAGTTACCC ACAAAACCTC 
AAAGTCGTTG GATTGACCAC AACCACCACA AAACTAGCCT GGGATCCTCC GCCGCTGCCC 
GAGAGGAATG GGAAGATCGT ACGATATGTG GTGGTTTATC GAGACATTAA CAGCCATCAG 
AATCAGACCA ACGGCACAGC GGACACGGAG ATGACCATTC AGGGCCTGAA GCCGGACACC 
ACCTATGATA TCAGGGTGAG GGCGTTTACA GGGAAAGGTG GAGGTCCCAT CAGCCCCAGC 
ATCCAGAGCA GAACCATGTC TACCTCCATG CCTGAGTTCA CGAAAAACTT TGGTGTGAAA 
GCCGTGATGA AGACCTCTGT CCTGCTCACA TGGGAGGTGC CAGAAACATA CAAGTCTCAA 
GTGCCTTTCA AGATCCTGTA TAACCAGCAG AGTGTGGAGG TCCAGGGTGA CCTGAAGAGG 
AAGCTGATTA CGCGTCTGCA GCCGGACACT GATTACTCCT TTGTCCTGAT GAGTCGGGGG 
AACAGCGCTG GCGGACTGCA GCAGCAGGTC TCCATCCGCA CCGCTCCAGA CCTGTTCACT 
ACCAAACCAG CACACCAGAA ACTAGACCCA GAGGAGGGCG GCAAACTCGC CATCACACTG 
CCCGAAGTGC ACAACAGCGC CCTCGTCAGG TGGTTTTATA TCGTGGTTGT ACCAGTAACT 
CCTTCAACTC TCCGCCATTG GGAAAACCCA GAAGATATTG ATCTGGATGA GCTGATGGGG 
AATAATAGAG ACGCAGCTGT GAGGAGGAGG AGAAGACAAA CTCAGGACTT TCTGCGGCCT 
TACATTGCTG CCAAGCTGGA GTCTCTTCCA GAAAGATTTG TGTTGGGAGA TGAGAAAACC 
TACAGCAACT ACTACAACAA ACCTCTGCCG GGACAGCAGC AGTATCGCAC ATTTATACTG 
GCGGCACTGA AAGACAGAGA CTCGACGTTC ACAGTGAGTC CGTTCTCTGA GCCCATGATG 
GTGAAGCTCC ACAGCGGGAT GTCCCGTCAC GTGGAGGACC CGGAGATGCT GTGGGTGATG 
GGACCAGTGT TAGCCGTGGT CCTCATCATC ATCATCGTCA TCGCCATCCT CCTCTTCAAG 
AGCAAACAGG AGAGGAAGCG CACGTCTCCC TCAGCTAAAG ATGAGCACTC AGCTGGAGTT 
AAAGATCACC TGCTGGCTCA TTCGTCAGAT CCAGTGGAGA TGAGGAGGCT CAACTACCAG 
ACACAAGGTT CCAGTGCCTT CAACTGTCCC AGCACTTCAA GAATGCGGGA ACACCCTCCA 
GTGTCTGTGT GTGCTTTGGC TGACCACATC GAGCGGCTGA GAGCCAACGA CAGCCTCCGC 
TTCTCGCAGG AGTATGAGTC TGTTGACCCT GGCCAGCAGT TCACCTGGGA AAACTCCAAC 
TTAGAGGTCA ACAAGCCCAA GAACCGCTAC GCAAACGTCA TCGCTTACGA TCACTCCAGG 
GTTGTGCTGA CTCCTGTGGA TGGTGTTCCC GGAAGTGACT ACATCAATGG AAACTACATT 
GATGGCTACC GTAAGCAGAA CGCCTACATC GCCACGCAGG GTCCGCTGCC TGAGACGCTC 
AGTGACTTCT GGAGGATGGT GTGGGAACAG AGAACCAGCA CTATCGTCAT GATGACCCGA 
CTGGAGGAGA AGAGCAGGGT GAAGTGTGAT CAGTACTGGC CAAGCCGAGG AACAGAGACT 
TACGGGATGA TTCAAGTGTC CATGCTGGAT ACGGTGGAGC TGGCCACATA CAGCGTCAGA 
ACCTTCGCTC TCTACAAGAA TGGATCCAGC GAGAAACGGG AGGTTCGGCA GTTCCAGTTC 
ATGGCGTGGC CGGATCACGG TGTTCCCGAA TATCCCACAC CCATTTTAGC CTTCTTACGC 
AGGGTCAAAG CCTGCAACCC ACCTGACGCG GGACCCATGG TGGTGCACTG CAGCGCTGGC 
GTGGGTCGGA CGGGCTGTTT CATCGTAATA GACGCCATGC TGGAGCGGAT GAAGCACGAG 
AAGTCGGTGG ACATCTACGG TCACGTGACC TGCATGCGTT CTCAGAGGAA CTACATGGTG 
CAGACAGAGG ATCAGTACAT TTTCATTCAC GAGGCCCTGC TGGAGGCTGC CACCTGCGGG 
AACACAGAGG TGCCGGCGCG GAGCCTCTAC ACACACATCC AGAAACTCAC ACAGGCGCCG 
CCTGGAGACA CCGTCACCGC CATGGAGCTC GAATTTAAGA AACTGGCCAA CTCTAAAGCA 
CACACCTCCA GATTCATCAG CGCTAGTCTG CCCTGCAACA AATTCAAGAA CCGGCTGGTG 
AACATCATGC CTTTCGAGTC CAGCCGTGTT TGTCTGCAGC CAATCAGAGG CGTGGAGGGC 
TCCGACTACA TCAACGCCAG CTTCATCGAC GGATACAGGC AGCAGAAGGC ATATATCGCC 
ACTCAAGGCC CGCTGTCTGA AACCACAGAG GACTTCTGGA GGATGCTTTG GGAGCACAAC 
TCCACCATCG TGGTGATGCT GACGAAGCTT AGAGAGATGG GACGGGAGAA ATGTCATCAG 
TACTGGCCTG CAGAGAGATC TGCGCGCTAC CAGTATTTCG TTGTGGATCC TATGGCTGAA 
TATAACATGC CACAGTACAT CTTACGGGAG TTTAAAGTCA CAGACGCCAG AGATGGCCAG 
TCTCGGACTA TCCGACAGTT TCAGTTCACA GATTGGCCGG AACAAGGTGT GCCAAAGACA 
GGAGAGGGCT TTATTGATTT TATTGGTCAA GTCCACAAAA CCAAGGAGCA GTTTGGACAG 
GACGGACCAA TTACTGTTCA CTGCAGTGCC GGTGTGGGCA GAACAGGCGT CTTCATCACT 
TTAAGTATTG TTCTGGAGCG GATGAGGTAT GAAGGAGTGG TGGACATGTT CCAGACCATA 
AAAACACTCC GCACGCAGAG ACCTGCCATG GTACAGACCG AGGACCAGTA CCAGCTGTGC 
TACAGAGCAG CTCTGGAGTA CCTGGGCAGC TTCGATCACT ATGCAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfa ( XM_021476769.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332444.1 Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X10, mRNA.

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CloneID	ODa142910
Clone ID Related Accession (Same CDS sequence)	XM_021476769.1
Accession Version	XM_021476769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGTCC GACGGGTTCC CCCACGTTTT 
TCAATCCCTC CAACCAATCA CGAGGTGATG CCGGGTGGCA GTGTGAACCT CACCTGCGTG 
GCAGTGGGCG CTCCAATGCC CTATGTGAAG TGGATGAAGG GCGAACAGGA GCTGACCAAA 
GAAGAGGAGA TGCCCATCGG CCGAAATGTG CTGGAGCTCA CCAACATCCG CCAGTCGGGG 
AACTACACCT GCGTGGCCAT ATCATCACTG GGCATGATCG ACACCACAGC GCAGATCACT 
GTTAAAGCTC TTCCTCGTCC TCCAGCCTCT CTGATTGTCA CTGAAACCAC CGCCACCAGT 
GTGACTTTAA CATGGGATTC TGGCAACCCA GAACCCGTAT CATATTACGT GATCCAGTAT 
CGATCCAAAA TCTCTGACAA TGGTTTCCAG GAGGTGGACG GCGTGGCATC CACCCGCTAC 
AGCATCGGCG GGTTGAGTCC GTACTCAGAG TATGAGTTTC GAGTAATGGC GGTCAATAAC 
ATTGGTCGCG GGCCGCCCAG CGGGATGGTG GAGACTCGTA CCAGTGAACA GGCACCGTCA 
TCTCCTCCGC TGCGCGTTCA AGCCCGGATG TTGAGCGCTA CGACTATGCT GGTGCAATGG 
GAACCTCCTG AAGAGCCCAA CGGACAGATT CGAGGATATC GAGTGTACTA CACGTCTGAC 
CTGCACTTCC CGCTCAGCAC ATGGCAGAAG CACAACACTG AGGACAGCAG TCTGACCACC 
ATCTCCGGCC TGGTTCCTGA CATCACGTAT GGGCTCCGTG TGCTGGCCTT CACCTCTGTT 
GGAGATGGAC CACCTTCCGA TATACTGCAG GTTAAAACAC AGCAAGGAGT CCCTGCTCAG 
CCGACTGGTT TTGAAGCTGA AGCCGAGTTG GACACGCGGA TCATGCTGTC ATGGTTGTGG 
CCCGTCCAAG ATCAGATCAC CAAATATGAG CTCACATACT GGGAGGCTGA CTCTGACAAC 
AAGCATCATG TCATCTTTGA CCCGGCCGGT TCATATGCTA TTGACAGCCT AAAGCCAGAC 
ACTCTCTATA AATTCTCTCT GGCGGCGCGC TCTGAGATGG GGCTCGGGGT TTACACACAG 
CCTATCGAGG CCCGCACAGC CCAGTCCGTG CCTGGTGCTC CTCCACGCAG GGTTGAGGTG 
GACAACATCA ACTCCACTGC ACTGCGTGTG TCCTGGAAGC CGCCTCTGCA GCAGAAACAA 
CACGGCCACA TACGTGGATA TCAGGTGGTT TACTCACGCC TGGAGAATGG AGAGCCGCGC 
GGACAGCCCA TCATTCTGGA CGTGGCGCTG CCAGAGGCAC AGGAGGCCGT GATCAGTGGT 
CTTCTCCCTG AAACCACATA TTCAGTGACT GTAGCCGCGT ACACCACTAA AGGAGATGGA 
GCGCGGAGCA AAGCCAAAGT GGTGACCACA ACAGGAGCAG TTCCAGGAAA GCCCACTATG 
ATCATCAGCA CCACCATAGG CAACACGGCT CTGATCCAGT GGCAGCCGCC TAAAGAGATG 
GTGGGAGAGC TGATGGGCTA CAGACTCCGC TATAAGCGTG AAGAAGAGGA TATTTACACT 
GTCCGAGACT TCAGACGCAC TGATGACCAC TTCACTGTCA CCGGCCTGCA CAAGGGCGCG 
ACGTACATCT TCAAACTCTG CGCTAAAAAC CGCGCCGGGA ATGGAGAGGA GTACAGCAAA 
GAGATCAGCA CCCCGGAGGA TGTGCCCAGC AGTTACCCAC AAAACCTCAA AGTCGTTGGA 
TTGACCACAA CCACCACAAA ACTAGCCTGG GATCCTCCGC CGCTGCCCGA GAGGAATGGG 
AAGATCGTAC GATATGTGGT GGTTTATCGA GACATTAACA GCCATCAGAA TCAGACCAAC 
GGCACAGCGG ACACGGAGAT GACCATTCAG GGCCTGAAGC CGGACACCAC CTATGATATC 
AGGGTGAGGG CGTTTACAGG GAAAGGTGGA GGTCCCATCA GCCCCAGCAT CCAGAGCAGA 
ACCATGTCTA CCTCCATGCC TGAGTTCACG AAAAACTTTG GTGTGAAAGC CGTGATGAAG 
ACCTCTGTCC TGCTCACATG GGAGGTGCCA GAAACATACA AGTCTCAAGT GCCTTTCAAG 
ATCCTGTATA ACCAGCAGAG TGTGGAGGTC CAGGGTGACC TGAAGAGGAA GCTGATTACG 
CGTCTGCAGC CGGACACTGA TTACTCCTTT GTCCTGATGA GTCGGGGGAA CAGCGCTGGC 
GGACTGCAGC AGCAGGTCTC CATCCGCACC GCTCCAGACC TGTTCACTAC CAAACCAGCA 
CACCAGAAAC TAGACCCAGA GGAGGGCGGC AAACTCGCCA TCACACTGCC CGAAGTGCAC 
AACAGCGCCC TCGTCAGGTG GTTTTATATC GTGGTTGTAC CAGTAACTCC TTCAACTCTC 
CGCCATTGGG AAAACCCAGA AGATATTGAT CTGGATGAGC TGATGGGGAA TAATAGAGAC 
GCAGCTGTGA GGAGGAGGAG AAGACAAACT CAGGACTTTC TGCGGCCTTA CATTGCTGCC 
AAGCTGGAGT CTCTTCCAGA AAGATTTGTG TTGGGAGATG AGAAAACCTA CAGCAACTAC 
TACAACAAAC CTCTGCCGGG ACAGCAGCAG TATCGCACAT TTATACTGGC GGCACTGAAA 
GACAGAGACT CGACGTTCAC AGTGAGTCCG TTCTCTGAGC CCATGATGGT GAAGCTCCAC 
AGCGGGATGT CCCGTCACGT GGAGGACCCG GAGATGCTGT GGGTGATGGG ACCAGTGTTA 
GCCGTGGTCC TCATCATCAT CATCGTCATC GCCATCCTCC TCTTCAAGAG CAAACAGGAG 
AGGAAGCGCA CGTCTCCCTC AGCTAAAGAT GAGCACTCAG CTGGAGTTAA AGATCACCTG 
CTGGCTCATT CGTCAGATCC AGTGGAGATG AGGAGGCTCA ACTACCAGAC ACAAGGTTCC 
AGTGCCTTCA ACTGTCCCAG CACTTCAAGA ATGCGGGAAC ACCCTCCAGT GTCTGTGTGT 
GCTTTGGCTG ACCACATCGA GCGGCTGAGA GCCAACGACA GCCTCCGCTT CTCGCAGGAG 
TATGAGTCTG TTGACCCTGG CCAGCAGTTC ACCTGGGAAA ACTCCAACTT AGAGGTCAAC 
AAGCCCAAGA ACCGCTACGC AAACGTCATC GCTTACGATC ACTCCAGGGT TGTGCTGACT 
CCTGTGGATG GTGTTCCCGG AAGTGACTAC ATCAATGGAA ACTACATTGA TGGCTACCGT 
AAGCAGAACG CCTACATCGC CACGCAGGGT CCGCTGCCTG AGACGCTCAG TGACTTCTGG 
AGGATGGTGT GGGAACAGAG AACCAGCACT ATCGTCATGA TGACCCGACT GGAGGAGAAG 
AGCAGGGTGA AGTGTGATCA GTACTGGCCA AGCCGAGGAA CAGAGACTTA CGGGATGATT 
CAAGTGTCCA TGCTGGATAC GGTGGAGCTG GCCACATACA GCGTCAGAAC CTTCGCTCTC 
TACAAGAATG GATCCAGCGA GAAACGGGAG GTTCGGCAGT TCCAGTTCAT GGCGTGGCCG 
GATCACGGTG TTCCCGAATA TCCCACACCC ATTTTAGCCT TCTTACGCAG GGTCAAAGCC 
TGCAACCCAC CTGACGCGGG ACCCATGGTG GTGCACTGCA GCGCTGGCGT GGGTCGGACG 
GGCTGTTTCA TCGTAATAGA CGCCATGCTG GAGCGGATGA AGCACGAGAA GTCGGTGGAC 
ATCTACGGTC ACGTGACCTG CATGCGTTCT CAGAGGAACT ACATGGTGCA GACAGAGGAT 
CAGTACATTT TCATTCACGA GGCCCTGCTG GAGGCTGCCA CCTGCGGGAA CACAGAGGTG 
CCGGCGCGGA GCCTCTACAC ACACATCCAG AAACTCACAC AGGCGCCGCC TGGAGACACC 
GTCACCGCCA TGGAGCTCGA ATTTAAGAAA CTGGCCAACT CTAAAGCACA CACCTCCAGA 
TTCATCAGCG CTAGTCTGCC CTGCAACAAA TTCAAGAACC GGCTGGTGAA CATCATGCCT 
TTCGAGTCCA GCCGTGTTTG TCTGCAGCCA ATCAGAGGCG TGGAGGGCTC CGACTACATC 
AACGCCAGCT TCATCGACGG ATACAGGCAG CAGAAGGCAT ATATCGCCAC TCAAGGCCCG 
CTGTCTGAAA CCACAGAGGA CTTCTGGAGG ATGCTTTGGG AGCACAACTC CACCATCGTG 
GTGATGCTGA CGAAGCTTAG AGAGATGGGA CGGGAGAAAT GTCATCAGTA CTGGCCTGCA 
GAGAGATCTG CGCGCTACCA GTATTTCGTT GTGGATCCTA TGGCTGAATA TAACATGCCA 
CAGTACATCT TACGGGAGTT TAAAGTCACA GACGCCAGAG ATGGCCAGTC TCGGACTATC 
CGACAGTTTC AGTTCACAGA TTGGCCGGAA CAAGGTGTGC CAAAGACAGG AGAGGGCTTT 
ATTGATTTTA TTGGTCAAGT CCACAAAACC AAGGAGCAGT TTGGACAGGA CGGACCAATT 
ACTGTTCACT GCAGTGCCGG TGTGGGCAGA ACAGGCGTCT TCATCACTTT AAGTATTGTT 
CTGGAGCGGA TGAGGTATGA AGGAGTGGTG GACATGTTCC AGACCATAAA AACACTCCGC 
ACGCAGAGAC CTGCCATGGT ACAGACCGAG GACCAGTACC AGCTGTGCTA CAGAGCAGCT 
CTGGAGTACC TGGGCAGCTT CGATCACTAT GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332444.1
CDS	1457..6895
Translation	MVPQTRQGSARHGATSLLLLLCLAVPIHADSMPSFVKTPEDQTGISGGVASFVCQAVGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEEDQIPHGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEISWFKDFLPVDINSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAVNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMKGEQELTKEEEMPIGRNVLELTNIRQSGNYTCVAISSLGMIDTTAQITVKALPRPPASLIVTETTATSVTLTWDSGNPEPVSYYVIQYRSKISDNGFQEVDGVASTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSGMVETRTSEQAPSSPPLRVQARMLSATTMLVQWEPPEEPNGQIRGYRVYYTSDLHFPLSTWQKHNTEDSSLTTISGLVPDITYGLRVLAFTSVGDGPPSDILQVKTQQGVPAQPTGFEAEAELDTRIMLSWLWPVQDQITKYELTYWEADSDNKHHVIFDPAGSYAIDSLKPDTLYKFSLAARSEMGLGVYTQPIEARTAQSVPGAPPRRVEVDNINSTALRVSWKPPLQQKQHGHIRGYQVVYSRLENGEPRGQPIILDVALPEAQEAVISGLLPETTYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTIGNTALIQWQPPKEMVGELMGYRLRYKREEEDIYTVRDFRRTDDHFTVTGLHKGATYIFKLCAKNRAGNGEEYSKEISTPEDVPSSYPQNLKVVGLTTTTTKLAWDPPPLPERNGKIVRYVVVYRDINSHQNQTNGTADTEMTIQGLKPDTTYDIRVRAFTGKGGGPISPSIQSRTMSTSMPEFTKNFGVKAVMKTSVLLTWEVPETYKSQVPFKILYNQQSVEVQGDLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFTTKPAHQKLDPEEGGKLAITLPEVHNSALVRWFYIVVVPVTPSTLRHWENPEDIDLDELMGNNRDAAVRRRRRQTQDFLRPYIAAKLESLPERFVLGDEKTYSNYYNKPLPGQQQYRTFILAALKDRDSTFTVSPFSEPMMVKLHSGMSRHVEDPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRTSPSAKDEHSAGVKDHLLAHSSDPVEMRRLNYQTQGSSAFNCPSTSRMREHPPVSVCALADHIERLRANDSLRFSQEYESVDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINGNYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRTSTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVSMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARSLYTHIQKLTQAPPGDTVTAMELEFKKLANSKAHTSRFISASLPCNKFKNRLVNIMPFESSRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLSETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTIKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021476769.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476769.1

ORF Insert Sequence:

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGTCC GACGGGTTCC CCCACGTTTT 
TCAATCCCTC CAACCAATCA CGAGGTGATG CCGGGTGGCA GTGTGAACCT CACCTGCGTG 
GCAGTGGGCG CTCCAATGCC CTATGTGAAG TGGATGAAGG GCGAACAGGA GCTGACCAAA 
GAAGAGGAGA TGCCCATCGG CCGAAATGTG CTGGAGCTCA CCAACATCCG CCAGTCGGGG 
AACTACACCT GCGTGGCCAT ATCATCACTG GGCATGATCG ACACCACAGC GCAGATCACT 
GTTAAAGCTC TTCCTCGTCC TCCAGCCTCT CTGATTGTCA CTGAAACCAC CGCCACCAGT 
GTGACTTTAA CATGGGATTC TGGCAACCCA GAACCCGTAT CATATTACGT GATCCAGTAT 
CGATCCAAAA TCTCTGACAA TGGTTTCCAG GAGGTGGACG GCGTGGCATC CACCCGCTAC 
AGCATCGGCG GGTTGAGTCC GTACTCAGAG TATGAGTTTC GAGTAATGGC GGTCAATAAC 
ATTGGTCGCG GGCCGCCCAG CGGGATGGTG GAGACTCGTA CCAGTGAACA GGCACCGTCA 
TCTCCTCCGC TGCGCGTTCA AGCCCGGATG TTGAGCGCTA CGACTATGCT GGTGCAATGG 
GAACCTCCTG AAGAGCCCAA CGGACAGATT CGAGGATATC GAGTGTACTA CACGTCTGAC 
CTGCACTTCC CGCTCAGCAC ATGGCAGAAG CACAACACTG AGGACAGCAG TCTGACCACC 
ATCTCCGGCC TGGTTCCTGA CATCACGTAT GGGCTCCGTG TGCTGGCCTT CACCTCTGTT 
GGAGATGGAC CACCTTCCGA TATACTGCAG GTTAAAACAC AGCAAGGAGT CCCTGCTCAG 
CCGACTGGTT TTGAAGCTGA AGCCGAGTTG GACACGCGGA TCATGCTGTC ATGGTTGTGG 
CCCGTCCAAG ATCAGATCAC CAAATATGAG CTCACATACT GGGAGGCTGA CTCTGACAAC 
AAGCATCATG TCATCTTTGA CCCGGCCGGT TCATATGCTA TTGACAGCCT AAAGCCAGAC 
ACTCTCTATA AATTCTCTCT GGCGGCGCGC TCTGAGATGG GGCTCGGGGT TTACACACAG 
CCTATCGAGG CCCGCACAGC CCAGTCCGTG CCTGGTGCTC CTCCACGCAG GGTTGAGGTG 
GACAACATCA ACTCCACTGC ACTGCGTGTG TCCTGGAAGC CGCCTCTGCA GCAGAAACAA 
CACGGCCACA TACGTGGATA TCAGGTGGTT TACTCACGCC TGGAGAATGG AGAGCCGCGC 
GGACAGCCCA TCATTCTGGA CGTGGCGCTG CCAGAGGCAC AGGAGGCCGT GATCAGTGGT 
CTTCTCCCTG AAACCACATA TTCAGTGACT GTAGCCGCGT ACACCACTAA AGGAGATGGA 
GCGCGGAGCA AAGCCAAAGT GGTGACCACA ACAGGAGCAG TTCCAGGAAA GCCCACTATG 
ATCATCAGCA CCACCATAGG CAACACGGCT CTGATCCAGT GGCAGCCGCC TAAAGAGATG 
GTGGGAGAGC TGATGGGCTA CAGACTCCGC TATAAGCGTG AAGAAGAGGA TATTTACACT 
GTCCGAGACT TCAGACGCAC TGATGACCAC TTCACTGTCA CCGGCCTGCA CAAGGGCGCG 
ACGTACATCT TCAAACTCTG CGCTAAAAAC CGCGCCGGGA ATGGAGAGGA GTACAGCAAA 
GAGATCAGCA CCCCGGAGGA TGTGCCCAGC AGTTACCCAC AAAACCTCAA AGTCGTTGGA 
TTGACCACAA CCACCACAAA ACTAGCCTGG GATCCTCCGC CGCTGCCCGA GAGGAATGGG 
AAGATCGTAC GATATGTGGT GGTTTATCGA GACATTAACA GCCATCAGAA TCAGACCAAC 
GGCACAGCGG ACACGGAGAT GACCATTCAG GGCCTGAAGC CGGACACCAC CTATGATATC 
AGGGTGAGGG CGTTTACAGG GAAAGGTGGA GGTCCCATCA GCCCCAGCAT CCAGAGCAGA 
ACCATGTCTA CCTCCATGCC TGAGTTCACG AAAAACTTTG GTGTGAAAGC CGTGATGAAG 
ACCTCTGTCC TGCTCACATG GGAGGTGCCA GAAACATACA AGTCTCAAGT GCCTTTCAAG 
ATCCTGTATA ACCAGCAGAG TGTGGAGGTC CAGGGTGACC TGAAGAGGAA GCTGATTACG 
CGTCTGCAGC CGGACACTGA TTACTCCTTT GTCCTGATGA GTCGGGGGAA CAGCGCTGGC 
GGACTGCAGC AGCAGGTCTC CATCCGCACC GCTCCAGACC TGTTCACTAC CAAACCAGCA 
CACCAGAAAC TAGACCCAGA GGAGGGCGGC AAACTCGCCA TCACACTGCC CGAAGTGCAC 
AACAGCGCCC TCGTCAGGTG GTTTTATATC GTGGTTGTAC CAGTAACTCC TTCAACTCTC 
CGCCATTGGG AAAACCCAGA AGATATTGAT CTGGATGAGC TGATGGGGAA TAATAGAGAC 
GCAGCTGTGA GGAGGAGGAG AAGACAAACT CAGGACTTTC TGCGGCCTTA CATTGCTGCC 
AAGCTGGAGT CTCTTCCAGA AAGATTTGTG TTGGGAGATG AGAAAACCTA CAGCAACTAC 
TACAACAAAC CTCTGCCGGG ACAGCAGCAG TATCGCACAT TTATACTGGC GGCACTGAAA 
GACAGAGACT CGACGTTCAC AGTGAGTCCG TTCTCTGAGC CCATGATGGT GAAGCTCCAC 
AGCGGGATGT CCCGTCACGT GGAGGACCCG GAGATGCTGT GGGTGATGGG ACCAGTGTTA 
GCCGTGGTCC TCATCATCAT CATCGTCATC GCCATCCTCC TCTTCAAGAG CAAACAGGAG 
AGGAAGCGCA CGTCTCCCTC AGCTAAAGAT GAGCACTCAG CTGGAGTTAA AGATCACCTG 
CTGGCTCATT CGTCAGATCC AGTGGAGATG AGGAGGCTCA ACTACCAGAC ACAAGGTTCC 
AGTGCCTTCA ACTGTCCCAG CACTTCAAGA ATGCGGGAAC ACCCTCCAGT GTCTGTGTGT 
GCTTTGGCTG ACCACATCGA GCGGCTGAGA GCCAACGACA GCCTCCGCTT CTCGCAGGAG 
TATGAGTCTG TTGACCCTGG CCAGCAGTTC ACCTGGGAAA ACTCCAACTT AGAGGTCAAC 
AAGCCCAAGA ACCGCTACGC AAACGTCATC GCTTACGATC ACTCCAGGGT TGTGCTGACT 
CCTGTGGATG GTGTTCCCGG AAGTGACTAC ATCAATGGAA ACTACATTGA TGGCTACCGT 
AAGCAGAACG CCTACATCGC CACGCAGGGT CCGCTGCCTG AGACGCTCAG TGACTTCTGG 
AGGATGGTGT GGGAACAGAG AACCAGCACT ATCGTCATGA TGACCCGACT GGAGGAGAAG 
AGCAGGGTGA AGTGTGATCA GTACTGGCCA AGCCGAGGAA CAGAGACTTA CGGGATGATT 
CAAGTGTCCA TGCTGGATAC GGTGGAGCTG GCCACATACA GCGTCAGAAC CTTCGCTCTC 
TACAAGAATG GATCCAGCGA GAAACGGGAG GTTCGGCAGT TCCAGTTCAT GGCGTGGCCG 
GATCACGGTG TTCCCGAATA TCCCACACCC ATTTTAGCCT TCTTACGCAG GGTCAAAGCC 
TGCAACCCAC CTGACGCGGG ACCCATGGTG GTGCACTGCA GCGCTGGCGT GGGTCGGACG 
GGCTGTTTCA TCGTAATAGA CGCCATGCTG GAGCGGATGA AGCACGAGAA GTCGGTGGAC 
ATCTACGGTC ACGTGACCTG CATGCGTTCT CAGAGGAACT ACATGGTGCA GACAGAGGAT 
CAGTACATTT TCATTCACGA GGCCCTGCTG GAGGCTGCCA CCTGCGGGAA CACAGAGGTG 
CCGGCGCGGA GCCTCTACAC ACACATCCAG AAACTCACAC AGGCGCCGCC TGGAGACACC 
GTCACCGCCA TGGAGCTCGA ATTTAAGAAA CTGGCCAACT CTAAAGCACA CACCTCCAGA 
TTCATCAGCG CTAGTCTGCC CTGCAACAAA TTCAAGAACC GGCTGGTGAA CATCATGCCT 
TTCGAGTCCA GCCGTGTTTG TCTGCAGCCA ATCAGAGGCG TGGAGGGCTC CGACTACATC 
AACGCCAGCT TCATCGACGG ATACAGGCAG CAGAAGGCAT ATATCGCCAC TCAAGGCCCG 
CTGTCTGAAA CCACAGAGGA CTTCTGGAGG ATGCTTTGGG AGCACAACTC CACCATCGTG 
GTGATGCTGA CGAAGCTTAG AGAGATGGGA CGGGAGAAAT GTCATCAGTA CTGGCCTGCA 
GAGAGATCTG CGCGCTACCA GTATTTCGTT GTGGATCCTA TGGCTGAATA TAACATGCCA 
CAGTACATCT TACGGGAGTT TAAAGTCACA GACGCCAGAG ATGGCCAGTC TCGGACTATC 
CGACAGTTTC AGTTCACAGA TTGGCCGGAA CAAGGTGTGC CAAAGACAGG AGAGGGCTTT 
ATTGATTTTA TTGGTCAAGT CCACAAAACC AAGGAGCAGT TTGGACAGGA CGGACCAATT 
ACTGTTCACT GCAGTGCCGG TGTGGGCAGA ACAGGCGTCT TCATCACTTT AAGTATTGTT 
CTGGAGCGGA TGAGGTATGA AGGAGTGGTG GACATGTTCC AGACCATAAA AACACTCCGC 
ACGCAGAGAC CTGCCATGGT ACAGACCGAG GACCAGTACC AGCTGTGCTA CAGAGCAGCT 
CTGGAGTACC TGGGCAGCTT CGATCACTAT GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfa ( XM_021476770.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332445.1 Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X11, mRNA.

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CloneID	ODa142911
Clone ID Related Accession (Same CDS sequence)	XM_021476770.1
Accession Version	XM_021476770.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGA GCGCTGCAGA TCGAGAACAG CGAGGAATCA 
GACCAGGGCA AGTATGAATG TGTGGCGGTG AACAGCGCGG GAACTCGATA CTCGGCTCCT 
GCAAACCTCT ACGTCAGAGT CCGACGGGTT CCCCCACGTT TTTCAATCCC TCCAACCAAT 
CACGAGGTGA TGCCGGGTGG CAGTGTGAAC CTCACCTGCG TGGCAGTGGG CGCTCCAATG 
CCCTATGTGA AGTGGATGAA GGGCGAACAG GAGCTGACCA AAGAAGAGGA GATGCCCATC 
GGCCGAAATG TGCTGGAGCT CACCAACATC CGCCAGTCGG GGAACTACAC CTGCGTGGCC 
ATATCATCAC TGGGCATGAT CGACACCACA GCGCAGATCA CTGTTAAAGC TCTTCCTCGT 
CCTCCAGCCT CTCTGATTGT CACTGAAACC ACCGCCACCA GTGTGACTTT AACATGGGAT 
TCTGGCAACC CAGAACCCGT ATCATATTAC GTGATCCAGT ATCGATCCAA AATCTCTGAC 
AATGGTTTCC AGGAGGTGGA CGGCGTGGCA TCCACCCGCT ACAGCATCGG CGGGTTGAGT 
CCGTACTCAG AGTATGAGTT TCGAGTAATG GCGGTCAATA ACATTGGTCG CGGGCCGCCC 
AGCGGGATGG TGGAGACTCG TACCAGTGAA CAGGCACCGT CATCTCCTCC GCTGCGCGTT 
CAAGCCCGGA TGTTGAGCGC TACGACTATG CTGGTGCAAT GGGAACCTCC TGAAGAGCCC 
AACGGACAGA TTCGAGGATA TCGAGTGTAC TACACGTCTG ACCTGCACTT CCCGCTCAGC 
ACATGGCAGA AGCACAACAC TGAGGACAGC AGTCTGACCA CCATCTCCGG CCTGGTTCCT 
GACATCACGT ATGGGCTCCG TGTGCTGGCC TTCACCTCTG TTGGAGATGG ACCACCTTCC 
GATATACTGC AGGTTAAAAC ACAGCAAGGA GTCCCTGCTC AGCCGACTGG TTTTGAAGCT 
GAAGCCGAGT TGGACACGCG GATCATGCTG TCATGGTTGT GGCCCGTCCA AGATCAGATC 
ACCAAATATG AGCTCACATA CTGGGAGGCT GACTCTGACA ACAAGCATCA TGTCATCTTT 
GACCCGGCCG GTTCATATGC TATTGACAGC CTAAAGCCAG ACACTCTCTA TAAATTCTCT 
CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG GTTTACACAC AGCCTATCGA GGCCCGCACA 
GCCCAGTCCG TGCCTGGTGC TCCTCCACGC AGGGTTGAGG TGGACAACAT CAACTCCACT 
GCACTGCGTG TGTCCTGGAA GCCGCCTCTG CAGCAGAAAC AACACGGCCA CATACGTGGA 
TATCAGGTGG TTTACTCACG CCTGGAGAAT GGAGAGCCGC GCGGACAGCC CATCATTCTG 
GACGTGGCGC TGCCAGAGGC ACAGGAGGCC GTGATCAGTG GTCTTCTCCC TGAAACCACA 
TATTCAGTGA CTGTAGCCGC GTACACCACT AAAGGAGATG GAGCGCGGAG CAAAGCCAAA 
GTGGTGACCA CAACAGGAGC AGTTCCAGGA AAGCCCACTA TGATCATCAG CACCACCATA 
GGCAACACGG CTCTGATCCA GTGGCAGCCG CCTAAAGAGA TGGTGGGAGA GCTGATGGGC 
TACAGACTCC GCTATAAGCG TGAAGAAGAG GATATTTACA CTGTCCGAGA CTTCAGACGC 
ACTGATGACC ACTTCACTGT CACCGGCCTG CACAAGGGCG CGACGTACAT CTTCAAACTC 
TGCGCTAAAA ACCGCGCCGG GAATGGAGAG GAGTACAGCA AAGAGATCAG CACCCCGGAG 
GATGTGCCCA GCAGTTACCC ACAAAACCTC AAAGTCGTTG GATTGACCAC AACCACCACA 
AAACTAGCCT GGGATCCTCC GCCGCTGCCC GAGAGGAATG GGAAGATCGT ACGATATGTG 
GTGGTTTATC GAGACATTAA CAGCCATCAG AATCAGACCA ACGGCACAGC GGACACGGAG 
ATGACCATTC AGGGCCTGAA GCCGGACACC ACCTATGATA TCAGGGTGAG GGCGTTTACA 
GGGAAAGGTG GAGGTCCCAT CAGCCCCAGC ATCCAGAGCA GAACCATGTC TACCTCCATG 
CCTGAGTTCA CGAAAAACTT TGGTGTGAAA GCCGTGATGA AGACCTCTGT CCTGCTCACA 
TGGGAGGTGC CAGAAACATA CAAGTCTCAA GTGCCTTTCA AGATCCTGTA TAACCAGCAG 
AGTGTGGAGG TCCAGGGTGA CCTGAAGAGG AAGCTGATTA CGCGTCTGCA GCCGGACACT 
GATTACTCCT TTGTCCTGAT GAGTCGGGGG AACAGCGCTG GCGGACTGCA GCAGCAGGTC 
TCCATCCGCA CCGCTCCAGA CCTGTTCACT ACCAAACCAG CACACCAGAA ACTAGACCCA 
GAGGAGGGCG GCAAACTCGC CATCACACTG CCCGAAGTGC ACAACAGCGC CCTCGTCAGG 
TGGTTTTATA TCGTGGTTGT ACCAGTAACT CCTTCAACTC TCCGCCATTG GGAAAACCCA 
GAAGATATTG ATCTGGATGA GCTGATGGGG AATAATAGAG ACGCAGCTGT GAGGAGGAGG 
AGAAGACAAA CTCAGGACTT TCTGCGGCCT TACATTGCTG CCAAGCTGGA GTCTCTTCCA 
GAAAGATTTG TGTTGGGAGA TGAGAAAACC TACAGCAACT ACTACAACAA ACCTCTGCCG 
GGACAGCAGC AGTATCGCAC ATTTATACTG GCGGCACTGA AAGACAGAGA CTCGACGTTC 
ACAGTGAGTC CGTTCTCTGA GCCCATGATG GTGAAGCTCC ACAGCGGGAT GTCCCGTCAC 
GTGGAGGACC CGGAGATGCT GTGGGTGATG GGACCAGTGT TAGCCGTGGT CCTCATCATC 
ATCATCGTCA TCGCCATCCT CCTCTTCAAG AGCAAACAGG AGAGGAAGCG CACGTCTCCC 
TCAGCTAAAG ATGAGCACTC AGCTGGAGTT AAAGATCACC TGCTGGCTCA TTCGTCAGAT 
CCAGTGGAGA TGAGGAGGCT CAACTACCAG ACACAAGGTT CCAGTGCCTT CAACTGTCCC 
AGCACTTCAA GAATGCGGGA ACACCCTCCA GTGTCTGTGT GTGCTTTGGC TGACCACATC 
GAGCGGCTGA GAGCCAACGA CAGCCTCCGC TTCTCGCAGG AGTATGAGTC TGTTGACCCT 
GGCCAGCAGT TCACCTGGGA AAACTCCAAC TTAGAGGTCA ACAAGCCCAA GAACCGCTAC 
GCAAACGTCA TCGCTTACGA TCACTCCAGG GTTGTGCTGA CTCCTGTGGA TGGTGTTCCC 
GGAAGTGACT ACATCAATGG AAACTACATT GATGGCTACC GTAAGCAGAA CGCCTACATC 
GCCACGCAGG GTCCGCTGCC TGAGACGCTC AGTGACTTCT GGAGGATGGT GTGGGAACAG 
AGAACCAGCA CTATCGTCAT GATGACCCGA CTGGAGGAGA AGAGCAGGGT GAAGTGTGAT 
CAGTACTGGC CAAGCCGAGG AACAGAGACT TACGGGATGA TTCAAGTGTC CATGCTGGAT 
ACGGTGGAGC TGGCCACATA CAGCGTCAGA ACCTTCGCTC TCTACAAGAA TGGATCCAGC 
GAGAAACGGG AGGTTCGGCA GTTCCAGTTC ATGGCGTGGC CGGATCACGG TGTTCCCGAA 
TATCCCACAC CCATTTTAGC CTTCTTACGC AGGGTCAAAG CCTGCAACCC ACCTGACGCG 
GGACCCATGG TGGTGCACTG CAGCGCTGGC GTGGGTCGGA CGGGCTGTTT CATCGTAATA 
GACGCCATGC TGGAGCGGAT GAAGCACGAG AAGTCGGTGG ACATCTACGG TCACGTGACC 
TGCATGCGTT CTCAGAGGAA CTACATGGTG CAGACAGAGG ATCAGTACAT TTTCATTCAC 
GAGGCCCTGC TGGAGGCTGC CACCTGCGGG AACACAGAGG TGCCGGCGCG GAGCCTCTAC 
ACACACATCC AGAAACTCAC ACAGGCGCCG CCTGGAGACA CCGTCACCGC CATGGAGCTC 
GAATTTAAGA AACTGGCCAA CTCTAAAGCA CACACCTCCA GATTCATCAG CGCTAGTCTG 
CCCTGCAACA AATTCAAGAA CCGGCTGGTG AACATCATGC CTTTCGAGTC CAGCCGTGTT 
TGTCTGCAGC CAATCAGAGG CGTGGAGGGC TCCGACTACA TCAACGCCAG CTTCATCGAC 
GGATACAGGC AGCAGAAGGC ATATATCGCC ACTCAAGGCC CGCTGTCTGA AACCACAGAG 
GACTTCTGGA GGATGCTTTG GGAGCACAAC TCCACCATCG TGGTGATGCT GACGAAGCTT 
AGAGAGATGG GACGGGAGAA ATGTCATCAG TACTGGCCTG CAGAGAGATC TGCGCGCTAC 
CAGTATTTCG TTGTGGATCC TATGGCTGAA TATAACATGC CACAGTACAT CTTACGGGAG 
TTTAAAGTCA CAGACGCCAG AGATGGCCAG TCTCGGACTA TCCGACAGTT TCAGTTCACA 
GATTGGCCGG AACAAGGTGT GCCAAAGACA GGAGAGGGCT TTATTGATTT TATTGGTCAA 
GTCCACAAAA CCAAGGAGCA GTTTGGACAG GACGGACCAA TTACTGTTCA CTGCAGTGCC 
GGTGTGGGCA GAACAGGCGT CTTCATCACT TTAAGTATTG TTCTGGAGCG GATGAGGTAT 
GAAGGAGTGG TGGACATGTT CCAGACCATA AAAACACTCC GCACGCAGAG ACCTGCCATG 
GTACAGACCG AGGACCAGTA CCAGCTGTGC TACAGAGCAG CTCTGGAGTA CCTGGGCAGC 
TTCGATCACT ATGCAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332445.1
CDS	1457..6877
Translation	MVPQTRQGSARHGATSLLLLLCLAVPIHADSMPSFVKTPEDQTGISGGVASFVCQAVGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEEDQIPHGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEISWFKDFLPVDINSSNGRIKQLRSGALQIENSEESDQGKYECVAVNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMKGEQELTKEEEMPIGRNVLELTNIRQSGNYTCVAISSLGMIDTTAQITVKALPRPPASLIVTETTATSVTLTWDSGNPEPVSYYVIQYRSKISDNGFQEVDGVASTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSGMVETRTSEQAPSSPPLRVQARMLSATTMLVQWEPPEEPNGQIRGYRVYYTSDLHFPLSTWQKHNTEDSSLTTISGLVPDITYGLRVLAFTSVGDGPPSDILQVKTQQGVPAQPTGFEAEAELDTRIMLSWLWPVQDQITKYELTYWEADSDNKHHVIFDPAGSYAIDSLKPDTLYKFSLAARSEMGLGVYTQPIEARTAQSVPGAPPRRVEVDNINSTALRVSWKPPLQQKQHGHIRGYQVVYSRLENGEPRGQPIILDVALPEAQEAVISGLLPETTYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTIGNTALIQWQPPKEMVGELMGYRLRYKREEEDIYTVRDFRRTDDHFTVTGLHKGATYIFKLCAKNRAGNGEEYSKEISTPEDVPSSYPQNLKVVGLTTTTTKLAWDPPPLPERNGKIVRYVVVYRDINSHQNQTNGTADTEMTIQGLKPDTTYDIRVRAFTGKGGGPISPSIQSRTMSTSMPEFTKNFGVKAVMKTSVLLTWEVPETYKSQVPFKILYNQQSVEVQGDLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFTTKPAHQKLDPEEGGKLAITLPEVHNSALVRWFYIVVVPVTPSTLRHWENPEDIDLDELMGNNRDAAVRRRRRQTQDFLRPYIAAKLESLPERFVLGDEKTYSNYYNKPLPGQQQYRTFILAALKDRDSTFTVSPFSEPMMVKLHSGMSRHVEDPEMLWVMGPVLAVVLIIIIVIAILLFKSKQERKRTSPSAKDEHSAGVKDHLLAHSSDPVEMRRLNYQTQGSSAFNCPSTSRMREHPPVSVCALADHIERLRANDSLRFSQEYESVDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINGNYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRTSTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVSMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARSLYTHIQKLTQAPPGDTVTAMELEFKKLANSKAHTSRFISASLPCNKFKNRLVNIMPFESSRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLSETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTIKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021476770.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476770.1

ORF Insert Sequence:

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGA GCGCTGCAGA TCGAGAACAG CGAGGAATCA 
GACCAGGGCA AGTATGAATG TGTGGCGGTG AACAGCGCGG GAACTCGATA CTCGGCTCCT 
GCAAACCTCT ACGTCAGAGT CCGACGGGTT CCCCCACGTT TTTCAATCCC TCCAACCAAT 
CACGAGGTGA TGCCGGGTGG CAGTGTGAAC CTCACCTGCG TGGCAGTGGG CGCTCCAATG 
CCCTATGTGA AGTGGATGAA GGGCGAACAG GAGCTGACCA AAGAAGAGGA GATGCCCATC 
GGCCGAAATG TGCTGGAGCT CACCAACATC CGCCAGTCGG GGAACTACAC CTGCGTGGCC 
ATATCATCAC TGGGCATGAT CGACACCACA GCGCAGATCA CTGTTAAAGC TCTTCCTCGT 
CCTCCAGCCT CTCTGATTGT CACTGAAACC ACCGCCACCA GTGTGACTTT AACATGGGAT 
TCTGGCAACC CAGAACCCGT ATCATATTAC GTGATCCAGT ATCGATCCAA AATCTCTGAC 
AATGGTTTCC AGGAGGTGGA CGGCGTGGCA TCCACCCGCT ACAGCATCGG CGGGTTGAGT 
CCGTACTCAG AGTATGAGTT TCGAGTAATG GCGGTCAATA ACATTGGTCG CGGGCCGCCC 
AGCGGGATGG TGGAGACTCG TACCAGTGAA CAGGCACCGT CATCTCCTCC GCTGCGCGTT 
CAAGCCCGGA TGTTGAGCGC TACGACTATG CTGGTGCAAT GGGAACCTCC TGAAGAGCCC 
AACGGACAGA TTCGAGGATA TCGAGTGTAC TACACGTCTG ACCTGCACTT CCCGCTCAGC 
ACATGGCAGA AGCACAACAC TGAGGACAGC AGTCTGACCA CCATCTCCGG CCTGGTTCCT 
GACATCACGT ATGGGCTCCG TGTGCTGGCC TTCACCTCTG TTGGAGATGG ACCACCTTCC 
GATATACTGC AGGTTAAAAC ACAGCAAGGA GTCCCTGCTC AGCCGACTGG TTTTGAAGCT 
GAAGCCGAGT TGGACACGCG GATCATGCTG TCATGGTTGT GGCCCGTCCA AGATCAGATC 
ACCAAATATG AGCTCACATA CTGGGAGGCT GACTCTGACA ACAAGCATCA TGTCATCTTT 
GACCCGGCCG GTTCATATGC TATTGACAGC CTAAAGCCAG ACACTCTCTA TAAATTCTCT 
CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG GTTTACACAC AGCCTATCGA GGCCCGCACA 
GCCCAGTCCG TGCCTGGTGC TCCTCCACGC AGGGTTGAGG TGGACAACAT CAACTCCACT 
GCACTGCGTG TGTCCTGGAA GCCGCCTCTG CAGCAGAAAC AACACGGCCA CATACGTGGA 
TATCAGGTGG TTTACTCACG CCTGGAGAAT GGAGAGCCGC GCGGACAGCC CATCATTCTG 
GACGTGGCGC TGCCAGAGGC ACAGGAGGCC GTGATCAGTG GTCTTCTCCC TGAAACCACA 
TATTCAGTGA CTGTAGCCGC GTACACCACT AAAGGAGATG GAGCGCGGAG CAAAGCCAAA 
GTGGTGACCA CAACAGGAGC AGTTCCAGGA AAGCCCACTA TGATCATCAG CACCACCATA 
GGCAACACGG CTCTGATCCA GTGGCAGCCG CCTAAAGAGA TGGTGGGAGA GCTGATGGGC 
TACAGACTCC GCTATAAGCG TGAAGAAGAG GATATTTACA CTGTCCGAGA CTTCAGACGC 
ACTGATGACC ACTTCACTGT CACCGGCCTG CACAAGGGCG CGACGTACAT CTTCAAACTC 
TGCGCTAAAA ACCGCGCCGG GAATGGAGAG GAGTACAGCA AAGAGATCAG CACCCCGGAG 
GATGTGCCCA GCAGTTACCC ACAAAACCTC AAAGTCGTTG GATTGACCAC AACCACCACA 
AAACTAGCCT GGGATCCTCC GCCGCTGCCC GAGAGGAATG GGAAGATCGT ACGATATGTG 
GTGGTTTATC GAGACATTAA CAGCCATCAG AATCAGACCA ACGGCACAGC GGACACGGAG 
ATGACCATTC AGGGCCTGAA GCCGGACACC ACCTATGATA TCAGGGTGAG GGCGTTTACA 
GGGAAAGGTG GAGGTCCCAT CAGCCCCAGC ATCCAGAGCA GAACCATGTC TACCTCCATG 
CCTGAGTTCA CGAAAAACTT TGGTGTGAAA GCCGTGATGA AGACCTCTGT CCTGCTCACA 
TGGGAGGTGC CAGAAACATA CAAGTCTCAA GTGCCTTTCA AGATCCTGTA TAACCAGCAG 
AGTGTGGAGG TCCAGGGTGA CCTGAAGAGG AAGCTGATTA CGCGTCTGCA GCCGGACACT 
GATTACTCCT TTGTCCTGAT GAGTCGGGGG AACAGCGCTG GCGGACTGCA GCAGCAGGTC 
TCCATCCGCA CCGCTCCAGA CCTGTTCACT ACCAAACCAG CACACCAGAA ACTAGACCCA 
GAGGAGGGCG GCAAACTCGC CATCACACTG CCCGAAGTGC ACAACAGCGC CCTCGTCAGG 
TGGTTTTATA TCGTGGTTGT ACCAGTAACT CCTTCAACTC TCCGCCATTG GGAAAACCCA 
GAAGATATTG ATCTGGATGA GCTGATGGGG AATAATAGAG ACGCAGCTGT GAGGAGGAGG 
AGAAGACAAA CTCAGGACTT TCTGCGGCCT TACATTGCTG CCAAGCTGGA GTCTCTTCCA 
GAAAGATTTG TGTTGGGAGA TGAGAAAACC TACAGCAACT ACTACAACAA ACCTCTGCCG 
GGACAGCAGC AGTATCGCAC ATTTATACTG GCGGCACTGA AAGACAGAGA CTCGACGTTC 
ACAGTGAGTC CGTTCTCTGA GCCCATGATG GTGAAGCTCC ACAGCGGGAT GTCCCGTCAC 
GTGGAGGACC CGGAGATGCT GTGGGTGATG GGACCAGTGT TAGCCGTGGT CCTCATCATC 
ATCATCGTCA TCGCCATCCT CCTCTTCAAG AGCAAACAGG AGAGGAAGCG CACGTCTCCC 
TCAGCTAAAG ATGAGCACTC AGCTGGAGTT AAAGATCACC TGCTGGCTCA TTCGTCAGAT 
CCAGTGGAGA TGAGGAGGCT CAACTACCAG ACACAAGGTT CCAGTGCCTT CAACTGTCCC 
AGCACTTCAA GAATGCGGGA ACACCCTCCA GTGTCTGTGT GTGCTTTGGC TGACCACATC 
GAGCGGCTGA GAGCCAACGA CAGCCTCCGC TTCTCGCAGG AGTATGAGTC TGTTGACCCT 
GGCCAGCAGT TCACCTGGGA AAACTCCAAC TTAGAGGTCA ACAAGCCCAA GAACCGCTAC 
GCAAACGTCA TCGCTTACGA TCACTCCAGG GTTGTGCTGA CTCCTGTGGA TGGTGTTCCC 
GGAAGTGACT ACATCAATGG AAACTACATT GATGGCTACC GTAAGCAGAA CGCCTACATC 
GCCACGCAGG GTCCGCTGCC TGAGACGCTC AGTGACTTCT GGAGGATGGT GTGGGAACAG 
AGAACCAGCA CTATCGTCAT GATGACCCGA CTGGAGGAGA AGAGCAGGGT GAAGTGTGAT 
CAGTACTGGC CAAGCCGAGG AACAGAGACT TACGGGATGA TTCAAGTGTC CATGCTGGAT 
ACGGTGGAGC TGGCCACATA CAGCGTCAGA ACCTTCGCTC TCTACAAGAA TGGATCCAGC 
GAGAAACGGG AGGTTCGGCA GTTCCAGTTC ATGGCGTGGC CGGATCACGG TGTTCCCGAA 
TATCCCACAC CCATTTTAGC CTTCTTACGC AGGGTCAAAG CCTGCAACCC ACCTGACGCG 
GGACCCATGG TGGTGCACTG CAGCGCTGGC GTGGGTCGGA CGGGCTGTTT CATCGTAATA 
GACGCCATGC TGGAGCGGAT GAAGCACGAG AAGTCGGTGG ACATCTACGG TCACGTGACC 
TGCATGCGTT CTCAGAGGAA CTACATGGTG CAGACAGAGG ATCAGTACAT TTTCATTCAC 
GAGGCCCTGC TGGAGGCTGC CACCTGCGGG AACACAGAGG TGCCGGCGCG GAGCCTCTAC 
ACACACATCC AGAAACTCAC ACAGGCGCCG CCTGGAGACA CCGTCACCGC CATGGAGCTC 
GAATTTAAGA AACTGGCCAA CTCTAAAGCA CACACCTCCA GATTCATCAG CGCTAGTCTG 
CCCTGCAACA AATTCAAGAA CCGGCTGGTG AACATCATGC CTTTCGAGTC CAGCCGTGTT 
TGTCTGCAGC CAATCAGAGG CGTGGAGGGC TCCGACTACA TCAACGCCAG CTTCATCGAC 
GGATACAGGC AGCAGAAGGC ATATATCGCC ACTCAAGGCC CGCTGTCTGA AACCACAGAG 
GACTTCTGGA GGATGCTTTG GGAGCACAAC TCCACCATCG TGGTGATGCT GACGAAGCTT 
AGAGAGATGG GACGGGAGAA ATGTCATCAG TACTGGCCTG CAGAGAGATC TGCGCGCTAC 
CAGTATTTCG TTGTGGATCC TATGGCTGAA TATAACATGC CACAGTACAT CTTACGGGAG 
TTTAAAGTCA CAGACGCCAG AGATGGCCAG TCTCGGACTA TCCGACAGTT TCAGTTCACA 
GATTGGCCGG AACAAGGTGT GCCAAAGACA GGAGAGGGCT TTATTGATTT TATTGGTCAA 
GTCCACAAAA CCAAGGAGCA GTTTGGACAG GACGGACCAA TTACTGTTCA CTGCAGTGCC 
GGTGTGGGCA GAACAGGCGT CTTCATCACT TTAAGTATTG TTCTGGAGCG GATGAGGTAT 
GAAGGAGTGG TGGACATGTT CCAGACCATA AAAACACTCC GCACGCAGAG ACCTGCCATG 
GTACAGACCG AGGACCAGTA CCAGCTGTGC TACAGAGCAG CTCTGGAGTA CCTGGGCAGC 
TTCGATCACT ATGCAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfa ( XM_021476771.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332446.1 Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X12, mRNA.

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CloneID	ODa142912
Clone ID Related Accession (Same CDS sequence)	XM_021476771.1
Accession Version	XM_021476771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGA GCGCTGCAGA TCGAGAACAG CGAGGAATCA 
GACCAGGGCA AGTATGAATG TGTGGCGGTG AACAGCGCGG GAACTCGATA CTCGGCTCCT 
GCAAACCTCT ACGTCAGAGT CCGACGGGTT CCCCCACGTT TTTCAATCCC TCCAACCAAT 
CACGAGGTGA TGCCGGGTGG CAGTGTGAAC CTCACCTGCG TGGCAGTGGG CGCTCCAATG 
CCCTATGTGA AGTGGATGAA GGGCGAACAG GAGCTGACCA AAGAAGAGGA GATGCCCATC 
GGCCGAAATG TGCTGGAGCT CACCAACATC CGCCAGTCGG GGAACTACAC CTGCGTGGCC 
ATATCATCAC TGGGCATGAT CGACACCACA GCGCAGATCA CTGTTAAAGC TCTTCCTCGT 
CCTCCAGCCT CTCTGATTGT CACTGAAACC ACCGCCACCA GTGTGACTTT AACATGGGAT 
TCTGGCAACC CAGAACCCGT ATCATATTAC GTGATCCAGT ATCGATCCAA AATCTCTGAC 
AATGGTTTCC AGGAGGTGGA CGGCGTGGCA TCCACCCGCT ACAGCATCGG CGGGTTGAGT 
CCGTACTCAG AGTATGAGTT TCGAGTAATG GCGGTCAATA ACATTGGTCG CGGGCCGCCC 
AGCGGGATGG TGGAGACTCG TACCAGTGAA CAGGCACCGT CATCTCCTCC GCTGCGCGTT 
CAAGCCCGGA TGTTGAGCGC TACGACTATG CTGGTGCAAT GGGAACCTCC TGAAGAGCCC 
AACGGACAGA TTCGAGGATA TCGAGTGTAC TACACGTCTG ACCTGCACTT CCCGCTCAGC 
ACATGGCAGA AGCACAACAC TGAGGACAGC AGTCTGACCA CCATCTCCGG CCTGGTTCCT 
GACATCACGT ATGGGCTCCG TGTGCTGGCC TTCACCTCTG TTGGAGATGG ACCACCTTCC 
GATATACTGC AGGTTAAAAC ACAGCAAGGA GTCCCTGCTC AGCCGACTGG TTTTGAAGCT 
GAAGCCGAGT TGGACACGCG GATCATGCTG TCATGGTTGT GGCCCGTCCA AGATCAGATC 
ACCAAATATG AGCTCACATA CTGGGAGGCT GACTCTGACA ACAAGCATCA TGTCATCTTT 
GACCCGGCCG GTTCATATGC TATTGACAGC CTAAAGCCAG ACACTCTCTA TAAATTCTCT 
CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG GTTTACACAC AGCCTATCGA GGCCCGCACA 
GCCCAGTCCG TGCCTGGTGC TCCTCCACGC AGGGTTGAGG TGGACAACAT CAACTCCACT 
GCACTGCGTG TGTCCTGGAA GCCGCCTCTG CAGCAGAAAC AACACGGCCA CATACGTGGA 
TATCAGGTGG TTTACTCACG CCTGGAGAAT GGAGAGCCGC GCGGACAGCC CATCATTCTG 
GACGTGGCGC TGCCAGAGGC ACAGGAGGCC GTGATCAGTG GTCTTCTCCC TGAAACCACA 
TATTCAGTGA CTGTAGCCGC GTACACCACT AAAGGAGATG GAGCGCGGAG CAAAGCCAAA 
GTGGTGACCA CAACAGGAGC AGTTCCAGGA AAGCCCACTA TGATCATCAG CACCACCATA 
GGCAACACGG CTCTGATCCA GTGGCAGCCG CCTAAAGAGA TGGTGGGAGA GCTGATGGGC 
TACAGACTCC GCTATAAGCG TGAAGAAGAG GATATTTACA CTGTCCGAGA CTTCAGACGC 
ACTGATGACC ACTTCACTGT CACCGGCCTG CACAAGGGCG CGACGTACAT CTTCAAACTC 
TGCGCTAAAA ACCGCGCCGG GAATGGAGAG GAGTACAGCA AAGAGATCAG CACCCCGGAG 
GATGTGCCCA GCAGTTACCC ACAAAACCTC AAAGTCGTTG GATTGACCAC AACCACCACA 
AAACTAGCCT GGGATCCTCC GCCGCTGCCC GAGAGGAATG GGAAGATCGT ACGATATGTG 
GTGGTTTATC GAGACATTAA CAGCCATCAG AATCAGACCA ACGGCACAGC GGACACGGAG 
ATGACCATTC AGGGCCTGAA GCCGGACACC ACCTATGATA TCAGGGTGAG GGCGTTTACA 
GGGAAAGGTG GAGGTCCCAT CAGCCCCAGC ATCCAGAGCA GAACCATGTC TACCTCCATG 
CCTGAGTTCA CGAAAAACTT TGGTGTGAAA GCCGTGATGA AGACCTCTGT CCTGCTCACA 
TGGGAGGTGC CAGAAACATA CAAGTCTCAA GTGCCTTTCA AGATCCTGTA TAACCAGCAG 
AGTGTGGAGG TCCAGGGTGA CCTGAAGAGG AAGCTGATTA CGCGTCTGCA GCCGGACACT 
GATTACTCCT TTGTCCTGAT GAGTCGGGGG AACAGCGCTG GCGGACTGCA GCAGCAGGTC 
TCCATCCGCA CCGCTCCAGA CCTGTTCACT ACCAAACCAG CACACCAGAA ACTAGACCCA 
GAGGAGGGCG GCAAACTCGC CATCACACTG CCCGAAGTGC ACAACAGCGC CCTCGTCAGG 
TGGTTTTATA TCGTGGTTGT ACCAGTAACT CCTTCAACTC TCCGCCATTG GGAAAACCCA 
GAAGATATTG ATCTGGATGA GCTGATGGGG AATAATAGAG ACGCAGCTGT GAGGAGGAGG 
AGAAGACAAA CTCAGGACTT TCTGCGGCCT TACATTGCTG CCAAGCTGGA GTCTCTTCCA 
GAAAGATTTG TGTTGGGAGA TGAGAAAACC TACAGCAACT ACTACAACAA ACCTCTGCCG 
GGACAGCAGC AGTATCGCAC ATTTATACTG GCGGCACTGA AAGACAGAGA CTCGACGTTC 
ACAGTGAGTC CGTTCTCTGA GCCCATGATG GTGAAGCTCC ACAGCGGGAT GTCCCGTCAC 
GTGGAGGACC CGGAGATGCT GTGGGTGATG GGACCAGTGT TAGCCGTGGT CCTCATCATC 
ATCATCGTCA TCGCCATCCT CCTCTTCAAG AGGAAGCGCA CGTCTCCCTC AGCTAAAGAT 
GAGCACTCAG CTGGAGTTAA AGATCACCTG CTGGCTCATT CGTCAGATCC AGTGGAGATG 
AGGAGGCTCA ACTACCAGAC ACAAGGAATG CGGGAACACC CTCCAGTGTC TGTGTGTGCT 
TTGGCTGACC ACATCGAGCG GCTGAGAGCC AACGACAGCC TCCGCTTCTC GCAGGAGTAT 
GAGTCTGTTG ACCCTGGCCA GCAGTTCACC TGGGAAAACT CCAACTTAGA GGTCAACAAG 
CCCAAGAACC GCTACGCAAA CGTCATCGCT TACGATCACT CCAGGGTTGT GCTGACTCCT 
GTGGATGGTG TTCCCGGAAG TGACTACATC AATGGAAACT ACATTGATGG CTACCGTAAG 
CAGAACGCCT ACATCGCCAC GCAGGGTCCG CTGCCTGAGA CGCTCAGTGA CTTCTGGAGG 
ATGGTGTGGG AACAGAGAAC CAGCACTATC GTCATGATGA CCCGACTGGA GGAGAAGAGC 
AGGGTGAAGT GTGATCAGTA CTGGCCAAGC CGAGGAACAG AGACTTACGG GATGATTCAA 
GTGTCCATGC TGGATACGGT GGAGCTGGCC ACATACAGCG TCAGAACCTT CGCTCTCTAC 
AAGAATGGAT CCAGCGAGAA ACGGGAGGTT CGGCAGTTCC AGTTCATGGC GTGGCCGGAT 
CACGGTGTTC CCGAATATCC CACACCCATT TTAGCCTTCT TACGCAGGGT CAAAGCCTGC 
AACCCACCTG ACGCGGGACC CATGGTGGTG CACTGCAGCG CTGGCGTGGG TCGGACGGGC 
TGTTTCATCG TAATAGACGC CATGCTGGAG CGGATGAAGC ACGAGAAGTC GGTGGACATC 
TACGGTCACG TGACCTGCAT GCGTTCTCAG AGGAACTACA TGGTGCAGAC AGAGGATCAG 
TACATTTTCA TTCACGAGGC CCTGCTGGAG GCTGCCACCT GCGGGAACAC AGAGGTGCCG 
GCGCGGAGCC TCTACACACA CATCCAGAAA CTCACACAGG CGCCGCCTGG AGACACCGTC 
ACCGCCATGG AGCTCGAATT TAAGAAACTG GCCAACTCTA AAGCACACAC CTCCAGATTC 
ATCAGCGCTA GTCTGCCCTG CAACAAATTC AAGAACCGGC TGGTGAACAT CATGCCTTTC 
GAGTCCAGCC GTGTTTGTCT GCAGCCAATC AGAGGCGTGG AGGGCTCCGA CTACATCAAC 
GCCAGCTTCA TCGACGGATA CAGGCAGCAG AAGGCATATA TCGCCACTCA AGGCCCGCTG 
TCTGAAACCA CAGAGGACTT CTGGAGGATG CTTTGGGAGC ACAACTCCAC CATCGTGGTG 
ATGCTGACGA AGCTTAGAGA GATGGGACGG GAGAAATGTC ATCAGTACTG GCCTGCAGAG 
AGATCTGCGC GCTACCAGTA TTTCGTTGTG GATCCTATGG CTGAATATAA CATGCCACAG 
TACATCTTAC GGGAGTTTAA AGTCACAGAC GCCAGAGATG GCCAGTCTCG GACTATCCGA 
CAGTTTCAGT TCACAGATTG GCCGGAACAA GGTGTGCCAA AGACAGGAGA GGGCTTTATT 
GATTTTATTG GTCAAGTCCA CAAAACCAAG GAGCAGTTTG GACAGGACGG ACCAATTACT 
GTTCACTGCA GTGCCGGTGT GGGCAGAACA GGCGTCTTCA TCACTTTAAG TATTGTTCTG 
GAGCGGATGA GGTATGAAGG AGTGGTGGAC ATGTTCCAGA CCATAAAAAC ACTCCGCACG 
CAGAGACCTG CCATGGTACA GACCGAGGAC CAGTACCAGC TGTGCTACAG AGCAGCTCTG 
GAGTACCTGG GCAGCTTCGA TCACTATGCA ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332446.1
CDS	307..5682
Translation	MVPQTRQGSARHGATSLLLLLCLAVPIHADSMPSFVKTPEDQTGISGGVASFVCQAVGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEEDQIPHGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEISWFKDFLPVDINSSNGRIKQLRSGALQIENSEESDQGKYECVAVNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMKGEQELTKEEEMPIGRNVLELTNIRQSGNYTCVAISSLGMIDTTAQITVKALPRPPASLIVTETTATSVTLTWDSGNPEPVSYYVIQYRSKISDNGFQEVDGVASTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSGMVETRTSEQAPSSPPLRVQARMLSATTMLVQWEPPEEPNGQIRGYRVYYTSDLHFPLSTWQKHNTEDSSLTTISGLVPDITYGLRVLAFTSVGDGPPSDILQVKTQQGVPAQPTGFEAEAELDTRIMLSWLWPVQDQITKYELTYWEADSDNKHHVIFDPAGSYAIDSLKPDTLYKFSLAARSEMGLGVYTQPIEARTAQSVPGAPPRRVEVDNINSTALRVSWKPPLQQKQHGHIRGYQVVYSRLENGEPRGQPIILDVALPEAQEAVISGLLPETTYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTIGNTALIQWQPPKEMVGELMGYRLRYKREEEDIYTVRDFRRTDDHFTVTGLHKGATYIFKLCAKNRAGNGEEYSKEISTPEDVPSSYPQNLKVVGLTTTTTKLAWDPPPLPERNGKIVRYVVVYRDINSHQNQTNGTADTEMTIQGLKPDTTYDIRVRAFTGKGGGPISPSIQSRTMSTSMPEFTKNFGVKAVMKTSVLLTWEVPETYKSQVPFKILYNQQSVEVQGDLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFTTKPAHQKLDPEEGGKLAITLPEVHNSALVRWFYIVVVPVTPSTLRHWENPEDIDLDELMGNNRDAAVRRRRRQTQDFLRPYIAAKLESLPERFVLGDEKTYSNYYNKPLPGQQQYRTFILAALKDRDSTFTVSPFSEPMMVKLHSGMSRHVEDPEMLWVMGPVLAVVLIIIIVIAILLFKRKRTSPSAKDEHSAGVKDHLLAHSSDPVEMRRLNYQTQGMREHPPVSVCALADHIERLRANDSLRFSQEYESVDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINGNYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRTSTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVSMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARSLYTHIQKLTQAPPGDTVTAMELEFKKLANSKAHTSRFISASLPCNKFKNRLVNIMPFESSRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLSETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTIKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021476771.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476771.1

ORF Insert Sequence:

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGA GCGCTGCAGA TCGAGAACAG CGAGGAATCA 
GACCAGGGCA AGTATGAATG TGTGGCGGTG AACAGCGCGG GAACTCGATA CTCGGCTCCT 
GCAAACCTCT ACGTCAGAGT CCGACGGGTT CCCCCACGTT TTTCAATCCC TCCAACCAAT 
CACGAGGTGA TGCCGGGTGG CAGTGTGAAC CTCACCTGCG TGGCAGTGGG CGCTCCAATG 
CCCTATGTGA AGTGGATGAA GGGCGAACAG GAGCTGACCA AAGAAGAGGA GATGCCCATC 
GGCCGAAATG TGCTGGAGCT CACCAACATC CGCCAGTCGG GGAACTACAC CTGCGTGGCC 
ATATCATCAC TGGGCATGAT CGACACCACA GCGCAGATCA CTGTTAAAGC TCTTCCTCGT 
CCTCCAGCCT CTCTGATTGT CACTGAAACC ACCGCCACCA GTGTGACTTT AACATGGGAT 
TCTGGCAACC CAGAACCCGT ATCATATTAC GTGATCCAGT ATCGATCCAA AATCTCTGAC 
AATGGTTTCC AGGAGGTGGA CGGCGTGGCA TCCACCCGCT ACAGCATCGG CGGGTTGAGT 
CCGTACTCAG AGTATGAGTT TCGAGTAATG GCGGTCAATA ACATTGGTCG CGGGCCGCCC 
AGCGGGATGG TGGAGACTCG TACCAGTGAA CAGGCACCGT CATCTCCTCC GCTGCGCGTT 
CAAGCCCGGA TGTTGAGCGC TACGACTATG CTGGTGCAAT GGGAACCTCC TGAAGAGCCC 
AACGGACAGA TTCGAGGATA TCGAGTGTAC TACACGTCTG ACCTGCACTT CCCGCTCAGC 
ACATGGCAGA AGCACAACAC TGAGGACAGC AGTCTGACCA CCATCTCCGG CCTGGTTCCT 
GACATCACGT ATGGGCTCCG TGTGCTGGCC TTCACCTCTG TTGGAGATGG ACCACCTTCC 
GATATACTGC AGGTTAAAAC ACAGCAAGGA GTCCCTGCTC AGCCGACTGG TTTTGAAGCT 
GAAGCCGAGT TGGACACGCG GATCATGCTG TCATGGTTGT GGCCCGTCCA AGATCAGATC 
ACCAAATATG AGCTCACATA CTGGGAGGCT GACTCTGACA ACAAGCATCA TGTCATCTTT 
GACCCGGCCG GTTCATATGC TATTGACAGC CTAAAGCCAG ACACTCTCTA TAAATTCTCT 
CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG GTTTACACAC AGCCTATCGA GGCCCGCACA 
GCCCAGTCCG TGCCTGGTGC TCCTCCACGC AGGGTTGAGG TGGACAACAT CAACTCCACT 
GCACTGCGTG TGTCCTGGAA GCCGCCTCTG CAGCAGAAAC AACACGGCCA CATACGTGGA 
TATCAGGTGG TTTACTCACG CCTGGAGAAT GGAGAGCCGC GCGGACAGCC CATCATTCTG 
GACGTGGCGC TGCCAGAGGC ACAGGAGGCC GTGATCAGTG GTCTTCTCCC TGAAACCACA 
TATTCAGTGA CTGTAGCCGC GTACACCACT AAAGGAGATG GAGCGCGGAG CAAAGCCAAA 
GTGGTGACCA CAACAGGAGC AGTTCCAGGA AAGCCCACTA TGATCATCAG CACCACCATA 
GGCAACACGG CTCTGATCCA GTGGCAGCCG CCTAAAGAGA TGGTGGGAGA GCTGATGGGC 
TACAGACTCC GCTATAAGCG TGAAGAAGAG GATATTTACA CTGTCCGAGA CTTCAGACGC 
ACTGATGACC ACTTCACTGT CACCGGCCTG CACAAGGGCG CGACGTACAT CTTCAAACTC 
TGCGCTAAAA ACCGCGCCGG GAATGGAGAG GAGTACAGCA AAGAGATCAG CACCCCGGAG 
GATGTGCCCA GCAGTTACCC ACAAAACCTC AAAGTCGTTG GATTGACCAC AACCACCACA 
AAACTAGCCT GGGATCCTCC GCCGCTGCCC GAGAGGAATG GGAAGATCGT ACGATATGTG 
GTGGTTTATC GAGACATTAA CAGCCATCAG AATCAGACCA ACGGCACAGC GGACACGGAG 
ATGACCATTC AGGGCCTGAA GCCGGACACC ACCTATGATA TCAGGGTGAG GGCGTTTACA 
GGGAAAGGTG GAGGTCCCAT CAGCCCCAGC ATCCAGAGCA GAACCATGTC TACCTCCATG 
CCTGAGTTCA CGAAAAACTT TGGTGTGAAA GCCGTGATGA AGACCTCTGT CCTGCTCACA 
TGGGAGGTGC CAGAAACATA CAAGTCTCAA GTGCCTTTCA AGATCCTGTA TAACCAGCAG 
AGTGTGGAGG TCCAGGGTGA CCTGAAGAGG AAGCTGATTA CGCGTCTGCA GCCGGACACT 
GATTACTCCT TTGTCCTGAT GAGTCGGGGG AACAGCGCTG GCGGACTGCA GCAGCAGGTC 
TCCATCCGCA CCGCTCCAGA CCTGTTCACT ACCAAACCAG CACACCAGAA ACTAGACCCA 
GAGGAGGGCG GCAAACTCGC CATCACACTG CCCGAAGTGC ACAACAGCGC CCTCGTCAGG 
TGGTTTTATA TCGTGGTTGT ACCAGTAACT CCTTCAACTC TCCGCCATTG GGAAAACCCA 
GAAGATATTG ATCTGGATGA GCTGATGGGG AATAATAGAG ACGCAGCTGT GAGGAGGAGG 
AGAAGACAAA CTCAGGACTT TCTGCGGCCT TACATTGCTG CCAAGCTGGA GTCTCTTCCA 
GAAAGATTTG TGTTGGGAGA TGAGAAAACC TACAGCAACT ACTACAACAA ACCTCTGCCG 
GGACAGCAGC AGTATCGCAC ATTTATACTG GCGGCACTGA AAGACAGAGA CTCGACGTTC 
ACAGTGAGTC CGTTCTCTGA GCCCATGATG GTGAAGCTCC ACAGCGGGAT GTCCCGTCAC 
GTGGAGGACC CGGAGATGCT GTGGGTGATG GGACCAGTGT TAGCCGTGGT CCTCATCATC 
ATCATCGTCA TCGCCATCCT CCTCTTCAAG AGGAAGCGCA CGTCTCCCTC AGCTAAAGAT 
GAGCACTCAG CTGGAGTTAA AGATCACCTG CTGGCTCATT CGTCAGATCC AGTGGAGATG 
AGGAGGCTCA ACTACCAGAC ACAAGGAATG CGGGAACACC CTCCAGTGTC TGTGTGTGCT 
TTGGCTGACC ACATCGAGCG GCTGAGAGCC AACGACAGCC TCCGCTTCTC GCAGGAGTAT 
GAGTCTGTTG ACCCTGGCCA GCAGTTCACC TGGGAAAACT CCAACTTAGA GGTCAACAAG 
CCCAAGAACC GCTACGCAAA CGTCATCGCT TACGATCACT CCAGGGTTGT GCTGACTCCT 
GTGGATGGTG TTCCCGGAAG TGACTACATC AATGGAAACT ACATTGATGG CTACCGTAAG 
CAGAACGCCT ACATCGCCAC GCAGGGTCCG CTGCCTGAGA CGCTCAGTGA CTTCTGGAGG 
ATGGTGTGGG AACAGAGAAC CAGCACTATC GTCATGATGA CCCGACTGGA GGAGAAGAGC 
AGGGTGAAGT GTGATCAGTA CTGGCCAAGC CGAGGAACAG AGACTTACGG GATGATTCAA 
GTGTCCATGC TGGATACGGT GGAGCTGGCC ACATACAGCG TCAGAACCTT CGCTCTCTAC 
AAGAATGGAT CCAGCGAGAA ACGGGAGGTT CGGCAGTTCC AGTTCATGGC GTGGCCGGAT 
CACGGTGTTC CCGAATATCC CACACCCATT TTAGCCTTCT TACGCAGGGT CAAAGCCTGC 
AACCCACCTG ACGCGGGACC CATGGTGGTG CACTGCAGCG CTGGCGTGGG TCGGACGGGC 
TGTTTCATCG TAATAGACGC CATGCTGGAG CGGATGAAGC ACGAGAAGTC GGTGGACATC 
TACGGTCACG TGACCTGCAT GCGTTCTCAG AGGAACTACA TGGTGCAGAC AGAGGATCAG 
TACATTTTCA TTCACGAGGC CCTGCTGGAG GCTGCCACCT GCGGGAACAC AGAGGTGCCG 
GCGCGGAGCC TCTACACACA CATCCAGAAA CTCACACAGG CGCCGCCTGG AGACACCGTC 
ACCGCCATGG AGCTCGAATT TAAGAAACTG GCCAACTCTA AAGCACACAC CTCCAGATTC 
ATCAGCGCTA GTCTGCCCTG CAACAAATTC AAGAACCGGC TGGTGAACAT CATGCCTTTC 
GAGTCCAGCC GTGTTTGTCT GCAGCCAATC AGAGGCGTGG AGGGCTCCGA CTACATCAAC 
GCCAGCTTCA TCGACGGATA CAGGCAGCAG AAGGCATATA TCGCCACTCA AGGCCCGCTG 
TCTGAAACCA CAGAGGACTT CTGGAGGATG CTTTGGGAGC ACAACTCCAC CATCGTGGTG 
ATGCTGACGA AGCTTAGAGA GATGGGACGG GAGAAATGTC ATCAGTACTG GCCTGCAGAG 
AGATCTGCGC GCTACCAGTA TTTCGTTGTG GATCCTATGG CTGAATATAA CATGCCACAG 
TACATCTTAC GGGAGTTTAA AGTCACAGAC GCCAGAGATG GCCAGTCTCG GACTATCCGA 
CAGTTTCAGT TCACAGATTG GCCGGAACAA GGTGTGCCAA AGACAGGAGA GGGCTTTATT 
GATTTTATTG GTCAAGTCCA CAAAACCAAG GAGCAGTTTG GACAGGACGG ACCAATTACT 
GTTCACTGCA GTGCCGGTGT GGGCAGAACA GGCGTCTTCA TCACTTTAAG TATTGTTCTG 
GAGCGGATGA GGTATGAAGG AGTGGTGGAC ATGTTCCAGA CCATAAAAAC ACTCCGCACG 
CAGAGACCTG CCATGGTACA GACCGAGGAC CAGTACCAGC TGTGCTACAG AGCAGCTCTG 
GAGTACCTGG GCAGCTTCGA TCACTATGCA ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfa ( XM_021476772.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332447.1 Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X13, mRNA.

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CloneID	ODa142913
Clone ID Related Accession (Same CDS sequence)	XM_021476772.1
Accession Version	XM_021476772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGTCC GACGGGTTCC CCCACGTTTT 
TCAATCCCTC CAACCAATCA CGAGGTGATG CCGGGTGGCA GTGTGAACCT CACCTGCGTG 
GCAGTGGGCG CTCCAATGCC CTATGTGAAG TGGATGAAGG GCGAACAGGA GCTGACCAAA 
GAAGAGGAGA TGCCCATCGG CCGAAATGTG CTGGAGCTCA CCAACATCCG CCAGTCGGGG 
AACTACACCT GCGTGGCCAT ATCATCACTG GGCATGATCG ACACCACAGC GCAGATCACT 
GTTAAAGCTC TTCCTCGTCC TCCAGCCTCT CTGATTGTCA CTGAAACCAC CGCCACCAGT 
GTGACTTTAA CATGGGATTC TGGCAACCCA GAACCCGTAT CATATTACGT GATCCAGTAT 
CGATCCAAAA TCTCTGACAA TGGTTTCCAG GAGGTGGACG GCGTGGCATC CACCCGCTAC 
AGCATCGGCG GGTTGAGTCC GTACTCAGAG TATGAGTTTC GAGTAATGGC GGTCAATAAC 
ATTGGTCGCG GGCCGCCCAG CGGGATGGTG GAGACTCGTA CCAGTGAACA GGCACCGTCA 
TCTCCTCCGC TGCGCGTTCA AGCCCGGATG TTGAGCGCTA CGACTATGCT GGTGCAATGG 
GAACCTCCTG AAGAGCCCAA CGGACAGATT CGAGGATATC GAGTGTACTA CACGTCTGAC 
CTGCACTTCC CGCTCAGCAC ATGGCAGAAG CACAACACTG AGGACAGCAG TCTGACCACC 
ATCTCCGGCC TGGTTCCTGA CATCACGTAT GGGCTCCGTG TGCTGGCCTT CACCTCTGTT 
GGAGATGGAC CACCTTCCGA TATACTGCAG GTTAAAACAC AGCAAGGAGT CCCTGCTCAG 
CCGACTGGTT TTGAAGCTGA AGCCGAGTTG GACACGCGGA TCATGCTGTC ATGGTTGTGG 
CCCGTCCAAG ATCAGATCAC CAAATATGAG CTCACATACT GGGAGGCTGA CTCTGACAAC 
AAGCATCATG TCATCTTTGA CCCGGCCGGT TCATATGCTA TTGACAGCCT AAAGCCAGAC 
ACTCTCTATA AATTCTCTCT GGCGGCGCGC TCTGAGATGG GGCTCGGGGT TTACACACAG 
CCTATCGAGG CCCGCACAGC CCAGTCCGTG CCTGGTGCTC CTCCACGCAG GGTTGAGGTG 
GACAACATCA ACTCCACTGC ACTGCGTGTG TCCTGGAAGC CGCCTCTGCA GCAGAAACAA 
CACGGCCACA TACGTGGATA TCAGGTGGTT TACTCACGCC TGGAGAATGG AGAGCCGCGC 
GGACAGCCCA TCATTCTGGA CGTGGCGCTG CCAGAGGCAC AGGAGGCCGT GATCAGTGGT 
CTTCTCCCTG AAACCACATA TTCAGTGACT GTAGCCGCGT ACACCACTAA AGGAGATGGA 
GCGCGGAGCA AAGCCAAAGT GGTGACCACA ACAGGAGCAG TTCCAGGAAA GCCCACTATG 
ATCATCAGCA CCACCATAGG CAACACGGCT CTGATCCAGT GGCAGCCGCC TAAAGAGATG 
GTGGGAGAGC TGATGGGCTA CAGACTCCGC TATAAGCGTG AAGAAGAGGA TATTTACACT 
GTCCGAGACT TCAGACGCAC TGATGACCAC TTCACTGTCA CCGGCCTGCA CAAGGGCGCG 
ACGTACATCT TCAAACTCTG CGCTAAAAAC CGCGCCGGGA ATGGAGAGGA GTACAGCAAA 
GAGATCAGCA CCCCGGAGGA TGTGCCCAGC AGTTACCCAC AAAACCTCAA AGTCGTTGGA 
TTGACCACAA CCACCACAAA ACTAGCCTGG GATCCTCCGC CGCTGCCCGA GAGGAATGGG 
AAGATCGTAC GATATGTGGT GGTTTATCGA GACATTAACA GCCATCAGAA TCAGACCAAC 
GGCACAGCGG ACACGGAGAT GACCATTCAG GGCCTGAAGC CGGACACCAC CTATGATATC 
AGGGTGAGGG CGTTTACAGG GAAAGGTGGA GGTCCCATCA GCCCCAGCAT CCAGAGCAGA 
ACCATGTCTA CCTCCATGCC TGAGTTCACG AAAAACTTTG GTGTGAAAGC CGTGATGAAG 
ACCTCTGTCC TGCTCACATG GGAGGTGCCA GAAACATACA AGTCTCAAGT GCCTTTCAAG 
ATCCTGTATA ACCAGCAGAG TGTGGAGGTC CAGGGTGACC TGAAGAGGAA GCTGATTACG 
CGTCTGCAGC CGGACACTGA TTACTCCTTT GTCCTGATGA GTCGGGGGAA CAGCGCTGGC 
GGACTGCAGC AGCAGGTCTC CATCCGCACC GCTCCAGACC TGTTCACTAC CAAACCAGCA 
CACCAGAAAC TAGACCCAGA GGAGGGCGGC AAACTCGCCA TCACACTGCC CGAAGTGCAC 
AACAGCGCCC TCGTCAGGTG GTTTTATATC GTGGTTGTAC CAGTAACTCC TTCAACTCTC 
CGCCATTGGG AAAACCCAGA AGATATTGAT CTGGATGAGC TGATGGGGAA TAATAGAGAC 
GCAGCTGTGA GGAGGAGGAG AAGACAAACT CAGGACTTTC TGCGGCCTTA CATTGCTGCC 
AAGCTGGAGT CTCTTCCAGA AAGATTTGTG TTGGGAGATG AGAAAACCTA CAGCAACTAC 
TACAACAAAC CTCTGCCGGG ACAGCAGCAG TATCGCACAT TTATACTGGC GGCACTGAAA 
GACAGAGACT CGACGTTCAC AGTGAGTCCG TTCTCTGAGC CCATGATGGT GAAGCTCCAC 
AGCGGGATGT CCCGTCACGT GGAGGACCCG GAGATGCTGT GGGTGATGGG ACCAGTGTTA 
GCCGTGGTCC TCATCATCAT CATCGTCATC GCCATCCTCC TCTTCAAGAG GAAGCGCACG 
TCTCCCTCAG CTAAAGATGA GCACTCAGCT GGAGTTAAAG ATCACCTGCT GGCTCATTCG 
TCAGATCCAG TGGAGATGAG GAGGCTCAAC TACCAGACAC AAGGTTCCAG TGCCTTCAAC 
TGTCCCAGCA CTTCAAGAAT GCGGGAACAC CCTCCAGTGT CTGTGTGTGC TTTGGCTGAC 
CACATCGAGC GGCTGAGAGC CAACGACAGC CTCCGCTTCT CGCAGGAGTA TGAGTCTGTT 
GACCCTGGCC AGCAGTTCAC CTGGGAAAAC TCCAACTTAG AGGTCAACAA GCCCAAGAAC 
CGCTACGCAA ACGTCATCGC TTACGATCAC TCCAGGGTTG TGCTGACTCC TGTGGATGGT 
GTTCCCGGAA GTGACTACAT CAATGGAAAC TACATTGATG GCTACCGTAA GCAGAACGCC 
TACATCGCCA CGCAGGGTCC GCTGCCTGAG ACGCTCAGTG ACTTCTGGAG GATGGTGTGG 
GAACAGAGAA CCAGCACTAT CGTCATGATG ACCCGACTGG AGGAGAAGAG CAGGGTGAAG 
TGTGATCAGT ACTGGCCAAG CCGAGGAACA GAGACTTACG GGATGATTCA AGTGTCCATG 
CTGGATACGG TGGAGCTGGC CACATACAGC GTCAGAACCT TCGCTCTCTA CAAGAATGGA 
TCCAGCGAGA AACGGGAGGT TCGGCAGTTC CAGTTCATGG CGTGGCCGGA TCACGGTGTT 
CCCGAATATC CCACACCCAT TTTAGCCTTC TTACGCAGGG TCAAAGCCTG CAACCCACCT 
GACGCGGGAC CCATGGTGGT GCACTGCAGC GCTGGCGTGG GTCGGACGGG CTGTTTCATC 
GTAATAGACG CCATGCTGGA GCGGATGAAG CACGAGAAGT CGGTGGACAT CTACGGTCAC 
GTGACCTGCA TGCGTTCTCA GAGGAACTAC ATGGTGCAGA CAGAGGATCA GTACATTTTC 
ATTCACGAGG CCCTGCTGGA GGCTGCCACC TGCGGGAACA CAGAGGTGCC GGCGCGGAGC 
CTCTACACAC ACATCCAGAA ACTCACACAG GCGCCGCCTG GAGACACCGT CACCGCCATG 
GAGCTCGAAT TTAAGAAACT GGCCAACTCT AAAGCACACA CCTCCAGATT CATCAGCGCT 
AGTCTGCCCT GCAACAAATT CAAGAACCGG CTGGTGAACA TCATGCCTTT CGAGTCCAGC 
CGTGTTTGTC TGCAGCCAAT CAGAGGCGTG GAGGGCTCCG ACTACATCAA CGCCAGCTTC 
ATCGACGGAT ACAGGCAGCA GAAGGCATAT ATCGCCACTC AAGGCCCGCT GTCTGAAACC 
ACAGAGGACT TCTGGAGGAT GCTTTGGGAG CACAACTCCA CCATCGTGGT GATGCTGACG 
AAGCTTAGAG AGATGGGACG GGAGAAATGT CATCAGTACT GGCCTGCAGA GAGATCTGCG 
CGCTACCAGT ATTTCGTTGT GGATCCTATG GCTGAATATA ACATGCCACA GTACATCTTA 
CGGGAGTTTA AAGTCACAGA CGCCAGAGAT GGCCAGTCTC GGACTATCCG ACAGTTTCAG 
TTCACAGATT GGCCGGAACA AGGTGTGCCA AAGACAGGAG AGGGCTTTAT TGATTTTATT 
GGTCAAGTCC ACAAAACCAA GGAGCAGTTT GGACAGGACG GACCAATTAC TGTTCACTGC 
AGTGCCGGTG TGGGCAGAAC AGGCGTCTTC ATCACTTTAA GTATTGTTCT GGAGCGGATG 
AGGTATGAAG GAGTGGTGGA CATGTTCCAG ACCATAAAAA CACTCCGCAC GCAGAGACCT 
GCCATGGTAC AGACCGAGGA CCAGTACCAG CTGTGCTACA GAGCAGCTCT GGAGTACCTG 
GGCAGCTTCG ATCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332447.1
CDS	1457..6883
Translation	MVPQTRQGSARHGATSLLLLLCLAVPIHADSMPSFVKTPEDQTGISGGVASFVCQAVGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEEDQIPHGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEISWFKDFLPVDINSSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVAVNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMKGEQELTKEEEMPIGRNVLELTNIRQSGNYTCVAISSLGMIDTTAQITVKALPRPPASLIVTETTATSVTLTWDSGNPEPVSYYVIQYRSKISDNGFQEVDGVASTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSGMVETRTSEQAPSSPPLRVQARMLSATTMLVQWEPPEEPNGQIRGYRVYYTSDLHFPLSTWQKHNTEDSSLTTISGLVPDITYGLRVLAFTSVGDGPPSDILQVKTQQGVPAQPTGFEAEAELDTRIMLSWLWPVQDQITKYELTYWEADSDNKHHVIFDPAGSYAIDSLKPDTLYKFSLAARSEMGLGVYTQPIEARTAQSVPGAPPRRVEVDNINSTALRVSWKPPLQQKQHGHIRGYQVVYSRLENGEPRGQPIILDVALPEAQEAVISGLLPETTYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTIGNTALIQWQPPKEMVGELMGYRLRYKREEEDIYTVRDFRRTDDHFTVTGLHKGATYIFKLCAKNRAGNGEEYSKEISTPEDVPSSYPQNLKVVGLTTTTTKLAWDPPPLPERNGKIVRYVVVYRDINSHQNQTNGTADTEMTIQGLKPDTTYDIRVRAFTGKGGGPISPSIQSRTMSTSMPEFTKNFGVKAVMKTSVLLTWEVPETYKSQVPFKILYNQQSVEVQGDLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFTTKPAHQKLDPEEGGKLAITLPEVHNSALVRWFYIVVVPVTPSTLRHWENPEDIDLDELMGNNRDAAVRRRRRQTQDFLRPYIAAKLESLPERFVLGDEKTYSNYYNKPLPGQQQYRTFILAALKDRDSTFTVSPFSEPMMVKLHSGMSRHVEDPEMLWVMGPVLAVVLIIIIVIAILLFKRKRTSPSAKDEHSAGVKDHLLAHSSDPVEMRRLNYQTQGSSAFNCPSTSRMREHPPVSVCALADHIERLRANDSLRFSQEYESVDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINGNYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRTSTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVSMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARSLYTHIQKLTQAPPGDTVTAMELEFKKLANSKAHTSRFISASLPCNKFKNRLVNIMPFESSRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLSETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTIKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021476772.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476772.1

ORF Insert Sequence:

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGT GGTACACCAA TCAGAGGAGC GCTGCAGATC 
GAGAACAGCG AGGAATCAGA CCAGGGCAAG TATGAATGTG TGGCGGTGAA CAGCGCGGGA 
ACTCGATACT CGGCTCCTGC AAACCTCTAC GTCAGAGTCC GACGGGTTCC CCCACGTTTT 
TCAATCCCTC CAACCAATCA CGAGGTGATG CCGGGTGGCA GTGTGAACCT CACCTGCGTG 
GCAGTGGGCG CTCCAATGCC CTATGTGAAG TGGATGAAGG GCGAACAGGA GCTGACCAAA 
GAAGAGGAGA TGCCCATCGG CCGAAATGTG CTGGAGCTCA CCAACATCCG CCAGTCGGGG 
AACTACACCT GCGTGGCCAT ATCATCACTG GGCATGATCG ACACCACAGC GCAGATCACT 
GTTAAAGCTC TTCCTCGTCC TCCAGCCTCT CTGATTGTCA CTGAAACCAC CGCCACCAGT 
GTGACTTTAA CATGGGATTC TGGCAACCCA GAACCCGTAT CATATTACGT GATCCAGTAT 
CGATCCAAAA TCTCTGACAA TGGTTTCCAG GAGGTGGACG GCGTGGCATC CACCCGCTAC 
AGCATCGGCG GGTTGAGTCC GTACTCAGAG TATGAGTTTC GAGTAATGGC GGTCAATAAC 
ATTGGTCGCG GGCCGCCCAG CGGGATGGTG GAGACTCGTA CCAGTGAACA GGCACCGTCA 
TCTCCTCCGC TGCGCGTTCA AGCCCGGATG TTGAGCGCTA CGACTATGCT GGTGCAATGG 
GAACCTCCTG AAGAGCCCAA CGGACAGATT CGAGGATATC GAGTGTACTA CACGTCTGAC 
CTGCACTTCC CGCTCAGCAC ATGGCAGAAG CACAACACTG AGGACAGCAG TCTGACCACC 
ATCTCCGGCC TGGTTCCTGA CATCACGTAT GGGCTCCGTG TGCTGGCCTT CACCTCTGTT 
GGAGATGGAC CACCTTCCGA TATACTGCAG GTTAAAACAC AGCAAGGAGT CCCTGCTCAG 
CCGACTGGTT TTGAAGCTGA AGCCGAGTTG GACACGCGGA TCATGCTGTC ATGGTTGTGG 
CCCGTCCAAG ATCAGATCAC CAAATATGAG CTCACATACT GGGAGGCTGA CTCTGACAAC 
AAGCATCATG TCATCTTTGA CCCGGCCGGT TCATATGCTA TTGACAGCCT AAAGCCAGAC 
ACTCTCTATA AATTCTCTCT GGCGGCGCGC TCTGAGATGG GGCTCGGGGT TTACACACAG 
CCTATCGAGG CCCGCACAGC CCAGTCCGTG CCTGGTGCTC CTCCACGCAG GGTTGAGGTG 
GACAACATCA ACTCCACTGC ACTGCGTGTG TCCTGGAAGC CGCCTCTGCA GCAGAAACAA 
CACGGCCACA TACGTGGATA TCAGGTGGTT TACTCACGCC TGGAGAATGG AGAGCCGCGC 
GGACAGCCCA TCATTCTGGA CGTGGCGCTG CCAGAGGCAC AGGAGGCCGT GATCAGTGGT 
CTTCTCCCTG AAACCACATA TTCAGTGACT GTAGCCGCGT ACACCACTAA AGGAGATGGA 
GCGCGGAGCA AAGCCAAAGT GGTGACCACA ACAGGAGCAG TTCCAGGAAA GCCCACTATG 
ATCATCAGCA CCACCATAGG CAACACGGCT CTGATCCAGT GGCAGCCGCC TAAAGAGATG 
GTGGGAGAGC TGATGGGCTA CAGACTCCGC TATAAGCGTG AAGAAGAGGA TATTTACACT 
GTCCGAGACT TCAGACGCAC TGATGACCAC TTCACTGTCA CCGGCCTGCA CAAGGGCGCG 
ACGTACATCT TCAAACTCTG CGCTAAAAAC CGCGCCGGGA ATGGAGAGGA GTACAGCAAA 
GAGATCAGCA CCCCGGAGGA TGTGCCCAGC AGTTACCCAC AAAACCTCAA AGTCGTTGGA 
TTGACCACAA CCACCACAAA ACTAGCCTGG GATCCTCCGC CGCTGCCCGA GAGGAATGGG 
AAGATCGTAC GATATGTGGT GGTTTATCGA GACATTAACA GCCATCAGAA TCAGACCAAC 
GGCACAGCGG ACACGGAGAT GACCATTCAG GGCCTGAAGC CGGACACCAC CTATGATATC 
AGGGTGAGGG CGTTTACAGG GAAAGGTGGA GGTCCCATCA GCCCCAGCAT CCAGAGCAGA 
ACCATGTCTA CCTCCATGCC TGAGTTCACG AAAAACTTTG GTGTGAAAGC CGTGATGAAG 
ACCTCTGTCC TGCTCACATG GGAGGTGCCA GAAACATACA AGTCTCAAGT GCCTTTCAAG 
ATCCTGTATA ACCAGCAGAG TGTGGAGGTC CAGGGTGACC TGAAGAGGAA GCTGATTACG 
CGTCTGCAGC CGGACACTGA TTACTCCTTT GTCCTGATGA GTCGGGGGAA CAGCGCTGGC 
GGACTGCAGC AGCAGGTCTC CATCCGCACC GCTCCAGACC TGTTCACTAC CAAACCAGCA 
CACCAGAAAC TAGACCCAGA GGAGGGCGGC AAACTCGCCA TCACACTGCC CGAAGTGCAC 
AACAGCGCCC TCGTCAGGTG GTTTTATATC GTGGTTGTAC CAGTAACTCC TTCAACTCTC 
CGCCATTGGG AAAACCCAGA AGATATTGAT CTGGATGAGC TGATGGGGAA TAATAGAGAC 
GCAGCTGTGA GGAGGAGGAG AAGACAAACT CAGGACTTTC TGCGGCCTTA CATTGCTGCC 
AAGCTGGAGT CTCTTCCAGA AAGATTTGTG TTGGGAGATG AGAAAACCTA CAGCAACTAC 
TACAACAAAC CTCTGCCGGG ACAGCAGCAG TATCGCACAT TTATACTGGC GGCACTGAAA 
GACAGAGACT CGACGTTCAC AGTGAGTCCG TTCTCTGAGC CCATGATGGT GAAGCTCCAC 
AGCGGGATGT CCCGTCACGT GGAGGACCCG GAGATGCTGT GGGTGATGGG ACCAGTGTTA 
GCCGTGGTCC TCATCATCAT CATCGTCATC GCCATCCTCC TCTTCAAGAG GAAGCGCACG 
TCTCCCTCAG CTAAAGATGA GCACTCAGCT GGAGTTAAAG ATCACCTGCT GGCTCATTCG 
TCAGATCCAG TGGAGATGAG GAGGCTCAAC TACCAGACAC AAGGTTCCAG TGCCTTCAAC 
TGTCCCAGCA CTTCAAGAAT GCGGGAACAC CCTCCAGTGT CTGTGTGTGC TTTGGCTGAC 
CACATCGAGC GGCTGAGAGC CAACGACAGC CTCCGCTTCT CGCAGGAGTA TGAGTCTGTT 
GACCCTGGCC AGCAGTTCAC CTGGGAAAAC TCCAACTTAG AGGTCAACAA GCCCAAGAAC 
CGCTACGCAA ACGTCATCGC TTACGATCAC TCCAGGGTTG TGCTGACTCC TGTGGATGGT 
GTTCCCGGAA GTGACTACAT CAATGGAAAC TACATTGATG GCTACCGTAA GCAGAACGCC 
TACATCGCCA CGCAGGGTCC GCTGCCTGAG ACGCTCAGTG ACTTCTGGAG GATGGTGTGG 
GAACAGAGAA CCAGCACTAT CGTCATGATG ACCCGACTGG AGGAGAAGAG CAGGGTGAAG 
TGTGATCAGT ACTGGCCAAG CCGAGGAACA GAGACTTACG GGATGATTCA AGTGTCCATG 
CTGGATACGG TGGAGCTGGC CACATACAGC GTCAGAACCT TCGCTCTCTA CAAGAATGGA 
TCCAGCGAGA AACGGGAGGT TCGGCAGTTC CAGTTCATGG CGTGGCCGGA TCACGGTGTT 
CCCGAATATC CCACACCCAT TTTAGCCTTC TTACGCAGGG TCAAAGCCTG CAACCCACCT 
GACGCGGGAC CCATGGTGGT GCACTGCAGC GCTGGCGTGG GTCGGACGGG CTGTTTCATC 
GTAATAGACG CCATGCTGGA GCGGATGAAG CACGAGAAGT CGGTGGACAT CTACGGTCAC 
GTGACCTGCA TGCGTTCTCA GAGGAACTAC ATGGTGCAGA CAGAGGATCA GTACATTTTC 
ATTCACGAGG CCCTGCTGGA GGCTGCCACC TGCGGGAACA CAGAGGTGCC GGCGCGGAGC 
CTCTACACAC ACATCCAGAA ACTCACACAG GCGCCGCCTG GAGACACCGT CACCGCCATG 
GAGCTCGAAT TTAAGAAACT GGCCAACTCT AAAGCACACA CCTCCAGATT CATCAGCGCT 
AGTCTGCCCT GCAACAAATT CAAGAACCGG CTGGTGAACA TCATGCCTTT CGAGTCCAGC 
CGTGTTTGTC TGCAGCCAAT CAGAGGCGTG GAGGGCTCCG ACTACATCAA CGCCAGCTTC 
ATCGACGGAT ACAGGCAGCA GAAGGCATAT ATCGCCACTC AAGGCCCGCT GTCTGAAACC 
ACAGAGGACT TCTGGAGGAT GCTTTGGGAG CACAACTCCA CCATCGTGGT GATGCTGACG 
AAGCTTAGAG AGATGGGACG GGAGAAATGT CATCAGTACT GGCCTGCAGA GAGATCTGCG 
CGCTACCAGT ATTTCGTTGT GGATCCTATG GCTGAATATA ACATGCCACA GTACATCTTA 
CGGGAGTTTA AAGTCACAGA CGCCAGAGAT GGCCAGTCTC GGACTATCCG ACAGTTTCAG 
TTCACAGATT GGCCGGAACA AGGTGTGCCA AAGACAGGAG AGGGCTTTAT TGATTTTATT 
GGTCAAGTCC ACAAAACCAA GGAGCAGTTT GGACAGGACG GACCAATTAC TGTTCACTGC 
AGTGCCGGTG TGGGCAGAAC AGGCGTCTTC ATCACTTTAA GTATTGTTCT GGAGCGGATG 
AGGTATGAAG GAGTGGTGGA CATGTTCCAG ACCATAAAAA CACTCCGCAC GCAGAGACCT 
GCCATGGTAC AGACCGAGGA CCAGTACCAG CTGTGCTACA GAGCAGCTCT GGAGTACCTG 
GGCAGCTTCG ATCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfa ( XM_021476773.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332448.1 Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X14, mRNA.

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CloneID	ODa142914
Clone ID Related Accession (Same CDS sequence)	XM_021476773.1
Accession Version	XM_021476773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGA GCGCTGCAGA TCGAGAACAG CGAGGAATCA 
GACCAGGGCA AGTATGAATG TGTGGCGGTG AACAGCGCGG GAACTCGATA CTCGGCTCCT 
GCAAACCTCT ACGTCAGAGT CCGACGGGTT CCCCCACGTT TTTCAATCCC TCCAACCAAT 
CACGAGGTGA TGCCGGGTGG CAGTGTGAAC CTCACCTGCG TGGCAGTGGG CGCTCCAATG 
CCCTATGTGA AGTGGATGAA GGGCGAACAG GAGCTGACCA AAGAAGAGGA GATGCCCATC 
GGCCGAAATG TGCTGGAGCT CACCAACATC CGCCAGTCGG GGAACTACAC CTGCGTGGCC 
ATATCATCAC TGGGCATGAT CGACACCACA GCGCAGATCA CTGTTAAAGC TCTTCCTCGT 
CCTCCAGCCT CTCTGATTGT CACTGAAACC ACCGCCACCA GTGTGACTTT AACATGGGAT 
TCTGGCAACC CAGAACCCGT ATCATATTAC GTGATCCAGT ATCGATCCAA AATCTCTGAC 
AATGGTTTCC AGGAGGTGGA CGGCGTGGCA TCCACCCGCT ACAGCATCGG CGGGTTGAGT 
CCGTACTCAG AGTATGAGTT TCGAGTAATG GCGGTCAATA ACATTGGTCG CGGGCCGCCC 
AGCGGGATGG TGGAGACTCG TACCAGTGAA CAGGCACCGT CATCTCCTCC GCTGCGCGTT 
CAAGCCCGGA TGTTGAGCGC TACGACTATG CTGGTGCAAT GGGAACCTCC TGAAGAGCCC 
AACGGACAGA TTCGAGGATA TCGAGTGTAC TACACGTCTG ACCTGCACTT CCCGCTCAGC 
ACATGGCAGA AGCACAACAC TGAGGACAGC AGTCTGACCA CCATCTCCGG CCTGGTTCCT 
GACATCACGT ATGGGCTCCG TGTGCTGGCC TTCACCTCTG TTGGAGATGG ACCACCTTCC 
GATATACTGC AGGTTAAAAC ACAGCAAGGA GTCCCTGCTC AGCCGACTGG TTTTGAAGCT 
GAAGCCGAGT TGGACACGCG GATCATGCTG TCATGGTTGT GGCCCGTCCA AGATCAGATC 
ACCAAATATG AGCTCACATA CTGGGAGGCT GACTCTGACA ACAAGCATCA TGTCATCTTT 
GACCCGGCCG GTTCATATGC TATTGACAGC CTAAAGCCAG ACACTCTCTA TAAATTCTCT 
CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG GTTTACACAC AGCCTATCGA GGCCCGCACA 
GCCCAGTCCG TGCCTGGTGC TCCTCCACGC AGGGTTGAGG TGGACAACAT CAACTCCACT 
GCACTGCGTG TGTCCTGGAA GCCGCCTCTG CAGCAGAAAC AACACGGCCA CATACGTGGA 
TATCAGGTGG TTTACTCACG CCTGGAGAAT GGAGAGCCGC GCGGACAGCC CATCATTCTG 
GACGTGGCGC TGCCAGAGGC ACAGGAGGCC GTGATCAGTG GTCTTCTCCC TGAAACCACA 
TATTCAGTGA CTGTAGCCGC GTACACCACT AAAGGAGATG GAGCGCGGAG CAAAGCCAAA 
GTGGTGACCA CAACAGGAGC AGTTCCAGGA AAGCCCACTA TGATCATCAG CACCACCATA 
GGCAACACGG CTCTGATCCA GTGGCAGCCG CCTAAAGAGA TGGTGGGAGA GCTGATGGGC 
TACAGACTCC GCTATAAGCG TGAAGAAGAG GATATTTACA CTGTCCGAGA CTTCAGACGC 
ACTGATGACC ACTTCACTGT CACCGGCCTG CACAAGGGCG CGACGTACAT CTTCAAACTC 
TGCGCTAAAA ACCGCGCCGG GAATGGAGAG GAGTACAGCA AAGAGATCAG CACCCCGGAG 
GATGTGCCCA GCAGTTACCC ACAAAACCTC AAAGTCGTTG GATTGACCAC AACCACCACA 
AAACTAGCCT GGGATCCTCC GCCGCTGCCC GAGAGGAATG GGAAGATCGT ACGATATGTG 
GTGGTTTATC GAGACATTAA CAGCCATCAG AATCAGACCA ACGGCACAGC GGACACGGAG 
ATGACCATTC AGGGCCTGAA GCCGGACACC ACCTATGATA TCAGGGTGAG GGCGTTTACA 
GGGAAAGGTG GAGGTCCCAT CAGCCCCAGC ATCCAGAGCA GAACCATGTC TACCTCCATG 
CCTGAGTTCA CGAAAAACTT TGGTGTGAAA GCCGTGATGA AGACCTCTGT CCTGCTCACA 
TGGGAGGTGC CAGAAACATA CAAGTCTCAA GTGCCTTTCA AGATCCTGTA TAACCAGCAG 
AGTGTGGAGG TCCAGGGTGA CCTGAAGAGG AAGCTGATTA CGCGTCTGCA GCCGGACACT 
GATTACTCCT TTGTCCTGAT GAGTCGGGGG AACAGCGCTG GCGGACTGCA GCAGCAGGTC 
TCCATCCGCA CCGCTCCAGA CCTGTTCACT ACCAAACCAG CACACCAGAA ACTAGACCCA 
GAGGAGGGCG GCAAACTCGC CATCACACTG CCCGAAGTGC ACAACAGCGC CCTCGTCAGG 
TGGTTTTATA TCGTGGTTGT ACCAGTAACT CCTTCAACTC TCCGCCATTG GGAAAACCCA 
GAAGATATTG ATCTGGATGA GCTGATGGGG AATAATAGAG ACGCAGCTGT GAGGAGGAGG 
AGAAGACAAA CTCAGGACTT TCTGCGGCCT TACATTGCTG CCAAGCTGGA GTCTCTTCCA 
GAAAGATTTG TGTTGGGAGA TGAGAAAACC TACAGCAACT ACTACAACAA ACCTCTGCCG 
GGACAGCAGC AGTATCGCAC ATTTATACTG GCGGCACTGA AAGACAGAGA CTCGACGTTC 
ACAGTGAGTC CGTTCTCTGA GCCCATGATG GTGAAGCTCC ACAGCGGGAT GTCCCGTCAC 
GTGGAGGACC CGGAGATGCT GTGGGTGATG GGACCAGTGT TAGCCGTGGT CCTCATCATC 
ATCATCGTCA TCGCCATCCT CCTCTTCAAG AGGAAGCGCA CGTCTCCCTC AGCTAAAGAT 
GAGCACTCAG CTGGAGTTAA AGATCACCTG CTGGCTCATT CGTCAGATCC AGTGGAGATG 
AGGAGGCTCA ACTACCAGAC ACAAGGTTCC AGTGCCTTCA ACTGTCCCAG CACTTCAAGA 
ATGCGGGAAC ACCCTCCAGT GTCTGTGTGT GCTTTGGCTG ACCACATCGA GCGGCTGAGA 
GCCAACGACA GCCTCCGCTT CTCGCAGGAG TATGAGTCTG TTGACCCTGG CCAGCAGTTC 
ACCTGGGAAA ACTCCAACTT AGAGGTCAAC AAGCCCAAGA ACCGCTACGC AAACGTCATC 
GCTTACGATC ACTCCAGGGT TGTGCTGACT CCTGTGGATG GTGTTCCCGG AAGTGACTAC 
ATCAATGGAA ACTACATTGA TGGCTACCGT AAGCAGAACG CCTACATCGC CACGCAGGGT 
CCGCTGCCTG AGACGCTCAG TGACTTCTGG AGGATGGTGT GGGAACAGAG AACCAGCACT 
ATCGTCATGA TGACCCGACT GGAGGAGAAG AGCAGGGTGA AGTGTGATCA GTACTGGCCA 
AGCCGAGGAA CAGAGACTTA CGGGATGATT CAAGTGTCCA TGCTGGATAC GGTGGAGCTG 
GCCACATACA GCGTCAGAAC CTTCGCTCTC TACAAGAATG GATCCAGCGA GAAACGGGAG 
GTTCGGCAGT TCCAGTTCAT GGCGTGGCCG GATCACGGTG TTCCCGAATA TCCCACACCC 
ATTTTAGCCT TCTTACGCAG GGTCAAAGCC TGCAACCCAC CTGACGCGGG ACCCATGGTG 
GTGCACTGCA GCGCTGGCGT GGGTCGGACG GGCTGTTTCA TCGTAATAGA CGCCATGCTG 
GAGCGGATGA AGCACGAGAA GTCGGTGGAC ATCTACGGTC ACGTGACCTG CATGCGTTCT 
CAGAGGAACT ACATGGTGCA GACAGAGGAT CAGTACATTT TCATTCACGA GGCCCTGCTG 
GAGGCTGCCA CCTGCGGGAA CACAGAGGTG CCGGCGCGGA GCCTCTACAC ACACATCCAG 
AAACTCACAC AGGCGCCGCC TGGAGACACC GTCACCGCCA TGGAGCTCGA ATTTAAGAAA 
CTGGCCAACT CTAAAGCACA CACCTCCAGA TTCATCAGCG CTAGTCTGCC CTGCAACAAA 
TTCAAGAACC GGCTGGTGAA CATCATGCCT TTCGAGTCCA GCCGTGTTTG TCTGCAGCCA 
ATCAGAGGCG TGGAGGGCTC CGACTACATC AACGCCAGCT TCATCGACGG ATACAGGCAG 
CAGAAGGCAT ATATCGCCAC TCAAGGCCCG CTGTCTGAAA CCACAGAGGA CTTCTGGAGG 
ATGCTTTGGG AGCACAACTC CACCATCGTG GTGATGCTGA CGAAGCTTAG AGAGATGGGA 
CGGGAGAAAT GTCATCAGTA CTGGCCTGCA GAGAGATCTG CGCGCTACCA GTATTTCGTT 
GTGGATCCTA TGGCTGAATA TAACATGCCA CAGTACATCT TACGGGAGTT TAAAGTCACA 
GACGCCAGAG ATGGCCAGTC TCGGACTATC CGACAGTTTC AGTTCACAGA TTGGCCGGAA 
CAAGGTGTGC CAAAGACAGG AGAGGGCTTT ATTGATTTTA TTGGTCAAGT CCACAAAACC 
AAGGAGCAGT TTGGACAGGA CGGACCAATT ACTGTTCACT GCAGTGCCGG TGTGGGCAGA 
ACAGGCGTCT TCATCACTTT AAGTATTGTT CTGGAGCGGA TGAGGTATGA AGGAGTGGTG 
GACATGTTCC AGACCATAAA AACACTCCGC ACGCAGAGAC CTGCCATGGT ACAGACCGAG 
GACCAGTACC AGCTGTGCTA CAGAGCAGCT CTGGAGTACC TGGGCAGCTT CGATCACTAT 
GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332448.1
CDS	1457..6865
Translation	MVPQTRQGSARHGATSLLLLLCLAVPIHADSMPSFVKTPEDQTGISGGVASFVCQAVGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEEDQIPHGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEISWFKDFLPVDINSSNGRIKQLRSGALQIENSEESDQGKYECVAVNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMKGEQELTKEEEMPIGRNVLELTNIRQSGNYTCVAISSLGMIDTTAQITVKALPRPPASLIVTETTATSVTLTWDSGNPEPVSYYVIQYRSKISDNGFQEVDGVASTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSGMVETRTSEQAPSSPPLRVQARMLSATTMLVQWEPPEEPNGQIRGYRVYYTSDLHFPLSTWQKHNTEDSSLTTISGLVPDITYGLRVLAFTSVGDGPPSDILQVKTQQGVPAQPTGFEAEAELDTRIMLSWLWPVQDQITKYELTYWEADSDNKHHVIFDPAGSYAIDSLKPDTLYKFSLAARSEMGLGVYTQPIEARTAQSVPGAPPRRVEVDNINSTALRVSWKPPLQQKQHGHIRGYQVVYSRLENGEPRGQPIILDVALPEAQEAVISGLLPETTYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTIGNTALIQWQPPKEMVGELMGYRLRYKREEEDIYTVRDFRRTDDHFTVTGLHKGATYIFKLCAKNRAGNGEEYSKEISTPEDVPSSYPQNLKVVGLTTTTTKLAWDPPPLPERNGKIVRYVVVYRDINSHQNQTNGTADTEMTIQGLKPDTTYDIRVRAFTGKGGGPISPSIQSRTMSTSMPEFTKNFGVKAVMKTSVLLTWEVPETYKSQVPFKILYNQQSVEVQGDLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFTTKPAHQKLDPEEGGKLAITLPEVHNSALVRWFYIVVVPVTPSTLRHWENPEDIDLDELMGNNRDAAVRRRRRQTQDFLRPYIAAKLESLPERFVLGDEKTYSNYYNKPLPGQQQYRTFILAALKDRDSTFTVSPFSEPMMVKLHSGMSRHVEDPEMLWVMGPVLAVVLIIIIVIAILLFKRKRTSPSAKDEHSAGVKDHLLAHSSDPVEMRRLNYQTQGSSAFNCPSTSRMREHPPVSVCALADHIERLRANDSLRFSQEYESVDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINGNYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRTSTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVSMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARSLYTHIQKLTQAPPGDTVTAMELEFKKLANSKAHTSRFISASLPCNKFKNRLVNIMPFESSRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLSETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTIKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_021476773.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f, a (ptprfa), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476773.1

ORF Insert Sequence:

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTCTGCCTGG CGGTGCCAAT CCACGCAGAC AGTATGCCCA GTTTCGTTAA GACTCCTGAA 
GATCAGACAG GGATTTCAGG AGGGGTGGCA TCATTCGTGT GCCAGGCGGT CGGAGAACCC 
AAACCTCGCA TTACCTGGAT GAAGAAGGGC AAGAAAGTCA GCTCTCAGCG CTTCGAGGTA 
ATAGAGTTTG ATGACGGCTC AGGCTCTGTG CTTCGGATCC AGCCATTACG GACACATCGA 
GATGAGGCGA TTTACGAGTG CACAGCTACC AACAGTGTTG GAGAAATTAA CACTAGTGCA 
AAACTCACTG TATTAGAAGA GGACCAGATC CCTCATGGTT TCCCCACTAT AGACATGGGT 
CCCCAGCTGA AAGTGGTTGA ACGGACGCGG ACGGCCACCA TGCTCTGTGC CGCCAGCGGA 
AACCCAGACC CGGAGATCTC CTGGTTCAAA GACTTTCTGC CTGTCGACAT CAACAGCAGC 
AACGGACGAA TCAAGCAGCT CCGTTCAGGA GCGCTGCAGA TCGAGAACAG CGAGGAATCA 
GACCAGGGCA AGTATGAATG TGTGGCGGTG AACAGCGCGG GAACTCGATA CTCGGCTCCT 
GCAAACCTCT ACGTCAGAGT CCGACGGGTT CCCCCACGTT TTTCAATCCC TCCAACCAAT 
CACGAGGTGA TGCCGGGTGG CAGTGTGAAC CTCACCTGCG TGGCAGTGGG CGCTCCAATG 
CCCTATGTGA AGTGGATGAA GGGCGAACAG GAGCTGACCA AAGAAGAGGA GATGCCCATC 
GGCCGAAATG TGCTGGAGCT CACCAACATC CGCCAGTCGG GGAACTACAC CTGCGTGGCC 
ATATCATCAC TGGGCATGAT CGACACCACA GCGCAGATCA CTGTTAAAGC TCTTCCTCGT 
CCTCCAGCCT CTCTGATTGT CACTGAAACC ACCGCCACCA GTGTGACTTT AACATGGGAT 
TCTGGCAACC CAGAACCCGT ATCATATTAC GTGATCCAGT ATCGATCCAA AATCTCTGAC 
AATGGTTTCC AGGAGGTGGA CGGCGTGGCA TCCACCCGCT ACAGCATCGG CGGGTTGAGT 
CCGTACTCAG AGTATGAGTT TCGAGTAATG GCGGTCAATA ACATTGGTCG CGGGCCGCCC 
AGCGGGATGG TGGAGACTCG TACCAGTGAA CAGGCACCGT CATCTCCTCC GCTGCGCGTT 
CAAGCCCGGA TGTTGAGCGC TACGACTATG CTGGTGCAAT GGGAACCTCC TGAAGAGCCC 
AACGGACAGA TTCGAGGATA TCGAGTGTAC TACACGTCTG ACCTGCACTT CCCGCTCAGC 
ACATGGCAGA AGCACAACAC TGAGGACAGC AGTCTGACCA CCATCTCCGG CCTGGTTCCT 
GACATCACGT ATGGGCTCCG TGTGCTGGCC TTCACCTCTG TTGGAGATGG ACCACCTTCC 
GATATACTGC AGGTTAAAAC ACAGCAAGGA GTCCCTGCTC AGCCGACTGG TTTTGAAGCT 
GAAGCCGAGT TGGACACGCG GATCATGCTG TCATGGTTGT GGCCCGTCCA AGATCAGATC 
ACCAAATATG AGCTCACATA CTGGGAGGCT GACTCTGACA ACAAGCATCA TGTCATCTTT 
GACCCGGCCG GTTCATATGC TATTGACAGC CTAAAGCCAG ACACTCTCTA TAAATTCTCT 
CTGGCGGCGC GCTCTGAGAT GGGGCTCGGG GTTTACACAC AGCCTATCGA GGCCCGCACA 
GCCCAGTCCG TGCCTGGTGC TCCTCCACGC AGGGTTGAGG TGGACAACAT CAACTCCACT 
GCACTGCGTG TGTCCTGGAA GCCGCCTCTG CAGCAGAAAC AACACGGCCA CATACGTGGA 
TATCAGGTGG TTTACTCACG CCTGGAGAAT GGAGAGCCGC GCGGACAGCC CATCATTCTG 
GACGTGGCGC TGCCAGAGGC ACAGGAGGCC GTGATCAGTG GTCTTCTCCC TGAAACCACA 
TATTCAGTGA CTGTAGCCGC GTACACCACT AAAGGAGATG GAGCGCGGAG CAAAGCCAAA 
GTGGTGACCA CAACAGGAGC AGTTCCAGGA AAGCCCACTA TGATCATCAG CACCACCATA 
GGCAACACGG CTCTGATCCA GTGGCAGCCG CCTAAAGAGA TGGTGGGAGA GCTGATGGGC 
TACAGACTCC GCTATAAGCG TGAAGAAGAG GATATTTACA CTGTCCGAGA CTTCAGACGC 
ACTGATGACC ACTTCACTGT CACCGGCCTG CACAAGGGCG CGACGTACAT CTTCAAACTC 
TGCGCTAAAA ACCGCGCCGG GAATGGAGAG GAGTACAGCA AAGAGATCAG CACCCCGGAG 
GATGTGCCCA GCAGTTACCC ACAAAACCTC AAAGTCGTTG GATTGACCAC AACCACCACA 
AAACTAGCCT GGGATCCTCC GCCGCTGCCC GAGAGGAATG GGAAGATCGT ACGATATGTG 
GTGGTTTATC GAGACATTAA CAGCCATCAG AATCAGACCA ACGGCACAGC GGACACGGAG 
ATGACCATTC AGGGCCTGAA GCCGGACACC ACCTATGATA TCAGGGTGAG GGCGTTTACA 
GGGAAAGGTG GAGGTCCCAT CAGCCCCAGC ATCCAGAGCA GAACCATGTC TACCTCCATG 
CCTGAGTTCA CGAAAAACTT TGGTGTGAAA GCCGTGATGA AGACCTCTGT CCTGCTCACA 
TGGGAGGTGC CAGAAACATA CAAGTCTCAA GTGCCTTTCA AGATCCTGTA TAACCAGCAG 
AGTGTGGAGG TCCAGGGTGA CCTGAAGAGG AAGCTGATTA CGCGTCTGCA GCCGGACACT 
GATTACTCCT TTGTCCTGAT GAGTCGGGGG AACAGCGCTG GCGGACTGCA GCAGCAGGTC 
TCCATCCGCA CCGCTCCAGA CCTGTTCACT ACCAAACCAG CACACCAGAA ACTAGACCCA 
GAGGAGGGCG GCAAACTCGC CATCACACTG CCCGAAGTGC ACAACAGCGC CCTCGTCAGG 
TGGTTTTATA TCGTGGTTGT ACCAGTAACT CCTTCAACTC TCCGCCATTG GGAAAACCCA 
GAAGATATTG ATCTGGATGA GCTGATGGGG AATAATAGAG ACGCAGCTGT GAGGAGGAGG 
AGAAGACAAA CTCAGGACTT TCTGCGGCCT TACATTGCTG CCAAGCTGGA GTCTCTTCCA 
GAAAGATTTG TGTTGGGAGA TGAGAAAACC TACAGCAACT ACTACAACAA ACCTCTGCCG 
GGACAGCAGC AGTATCGCAC ATTTATACTG GCGGCACTGA AAGACAGAGA CTCGACGTTC 
ACAGTGAGTC CGTTCTCTGA GCCCATGATG GTGAAGCTCC ACAGCGGGAT GTCCCGTCAC 
GTGGAGGACC CGGAGATGCT GTGGGTGATG GGACCAGTGT TAGCCGTGGT CCTCATCATC 
ATCATCGTCA TCGCCATCCT CCTCTTCAAG AGGAAGCGCA CGTCTCCCTC AGCTAAAGAT 
GAGCACTCAG CTGGAGTTAA AGATCACCTG CTGGCTCATT CGTCAGATCC AGTGGAGATG 
AGGAGGCTCA ACTACCAGAC ACAAGGTTCC AGTGCCTTCA ACTGTCCCAG CACTTCAAGA 
ATGCGGGAAC ACCCTCCAGT GTCTGTGTGT GCTTTGGCTG ACCACATCGA GCGGCTGAGA 
GCCAACGACA GCCTCCGCTT CTCGCAGGAG TATGAGTCTG TTGACCCTGG CCAGCAGTTC 
ACCTGGGAAA ACTCCAACTT AGAGGTCAAC AAGCCCAAGA ACCGCTACGC AAACGTCATC 
GCTTACGATC ACTCCAGGGT TGTGCTGACT CCTGTGGATG GTGTTCCCGG AAGTGACTAC 
ATCAATGGAA ACTACATTGA TGGCTACCGT AAGCAGAACG CCTACATCGC CACGCAGGGT 
CCGCTGCCTG AGACGCTCAG TGACTTCTGG AGGATGGTGT GGGAACAGAG AACCAGCACT 
ATCGTCATGA TGACCCGACT GGAGGAGAAG AGCAGGGTGA AGTGTGATCA GTACTGGCCA 
AGCCGAGGAA CAGAGACTTA CGGGATGATT CAAGTGTCCA TGCTGGATAC GGTGGAGCTG 
GCCACATACA GCGTCAGAAC CTTCGCTCTC TACAAGAATG GATCCAGCGA GAAACGGGAG 
GTTCGGCAGT TCCAGTTCAT GGCGTGGCCG GATCACGGTG TTCCCGAATA TCCCACACCC 
ATTTTAGCCT TCTTACGCAG GGTCAAAGCC TGCAACCCAC CTGACGCGGG ACCCATGGTG 
GTGCACTGCA GCGCTGGCGT GGGTCGGACG GGCTGTTTCA TCGTAATAGA CGCCATGCTG 
GAGCGGATGA AGCACGAGAA GTCGGTGGAC ATCTACGGTC ACGTGACCTG CATGCGTTCT 
CAGAGGAACT ACATGGTGCA GACAGAGGAT CAGTACATTT TCATTCACGA GGCCCTGCTG 
GAGGCTGCCA CCTGCGGGAA CACAGAGGTG CCGGCGCGGA GCCTCTACAC ACACATCCAG 
AAACTCACAC AGGCGCCGCC TGGAGACACC GTCACCGCCA TGGAGCTCGA ATTTAAGAAA 
CTGGCCAACT CTAAAGCACA CACCTCCAGA TTCATCAGCG CTAGTCTGCC CTGCAACAAA 
TTCAAGAACC GGCTGGTGAA CATCATGCCT TTCGAGTCCA GCCGTGTTTG TCTGCAGCCA 
ATCAGAGGCG TGGAGGGCTC CGACTACATC AACGCCAGCT TCATCGACGG ATACAGGCAG 
CAGAAGGCAT ATATCGCCAC TCAAGGCCCG CTGTCTGAAA CCACAGAGGA CTTCTGGAGG 
ATGCTTTGGG AGCACAACTC CACCATCGTG GTGATGCTGA CGAAGCTTAG AGAGATGGGA 
CGGGAGAAAT GTCATCAGTA CTGGCCTGCA GAGAGATCTG CGCGCTACCA GTATTTCGTT 
GTGGATCCTA TGGCTGAATA TAACATGCCA CAGTACATCT TACGGGAGTT TAAAGTCACA 
GACGCCAGAG ATGGCCAGTC TCGGACTATC CGACAGTTTC AGTTCACAGA TTGGCCGGAA 
CAAGGTGTGC CAAAGACAGG AGAGGGCTTT ATTGATTTTA TTGGTCAAGT CCACAAAACC 
AAGGAGCAGT TTGGACAGGA CGGACCAATT ACTGTTCACT GCAGTGCCGG TGTGGGCAGA 
ACAGGCGTCT TCATCACTTT AAGTATTGTT CTGGAGCGGA TGAGGTATGA AGGAGTGGTG 
GACATGTTCC AGACCATAAA AACACTCCGC ACGCAGAGAC CTGCCATGGT ACAGACCGAG 
GACCAGTACC AGCTGTGCTA CAGAGCAGCT CTGGAGTACC TGGGCAGCTT CGATCACTAT 
GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprfa ( NM_001311348.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001298277.1 Danio rerio protein tyrosine phosphatase receptor type Fa (ptprfa), mRNA.

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CloneID	ODa39492
Clone ID Related Accession (Same CDS sequence)	NM_001311348.1
Accession Version	NM_001311348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	receptor-type tyrosine-protein phosphatase F precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from JQ414007.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JQ414007.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTGCTCTGCC TGGCGGTGCC AATCCACGCA GACAGTATGC CCAGTTTCGT TAAGACTCCT 
GAAGATCAGA CAGGGATTTC AGGAGGGGTG GCATCATTCG TGTGCCAGGC GGTCGGAGAA 
CCCAAACCTC GCATTACCTG GATGAAGAAG GGCAAGAAAG TCAGCTCTCA GCGCTTCGAG 
GTAATAGAGT TTGATGACGG CTCAGGCTCT GTGCTTCGGA TCCAGCCATT ACGGACACAT 
CGAGATGAGG CGATTTACGA GTGCACAGCT ACCAACAGTG TTGGAGAAAT TAACACTAGT 
GCAAAACTCA CTGTATTAGA AGAGGACCAG ATCCCTCATG GTTTCCCCAC TATAGACATG 
GGTCCCCAGC TGAAAGTGGT TGAACGGACG CGGACGGCCA CCATGCTCTG TGCCGCCAGC 
GGAAACCCAG ACCCGGAGAT CTCCTGGTTC AAAGACTTTC TGCCTGTCGA CATCAACAGC 
AGCAACGGAC GAATCAAGCA GCTCCGTTCA GGAGCGCTGC AGATCGAGAA CAGCGAGGAA 
TCAGACCAGG GCAAGTATGA ATGTGTGGCG GTGAACAGCG CGGGAACTCG ATACTCGGCT 
CCTGCAAACC TCTACGTCAG AGTCCGACGG GTTCCCCCAC GTTTTTCAAT CCCTCCAACC 
AATCACGAGG TGATGCCGGG TGGCAGTGTG AACCTCACCT GCGTGGCAGT GGGCGCTCCA 
ATGCCCTATG TGAAGTGGAT GAAGGGCGAA CAGGAGCTGA CCAAAGAAGA GGAGATGCCC 
ATTGGCCGAA ATGTGCTGGA GCTCACCAAC ATCCGCCAGT CGGGGAACTA CACCTGCGTG 
GCCATATCAT CACTGGGCAT GATCGACACC ACAGCGCAGA TCACTGTTAA AGCTCTTCCT 
CGTCCTCCAG CCTCTCTGAT TGTCACTGAA ACCACCGCCA CCAGTGTGAC TTTAACATGG 
GATTCTGGCA ACCCAGAACC CGTATCATAT TACGTGATCC AGTATCGATC CAAAATCTCT 
GACAATGGTT TCCAGGAGGT GGACGGCGTG GCATCCACCC GCTACAGCAT CGGCGGGTTG 
AGTCCGTACT CAGAGTATGA GTTTCGAGTA ATGGCGGTCA ATAACATTGG TCGCGGGCCG 
CCCAGCGGGA TGGTGGAGAC TCGTACCAGT GAACAGGCAC CGTCATCTCC TCCGCTGCGC 
GTTCAAGCCC GGATGTTGAG CGCTACGACT ATGCTGGTGC AATGGGAACC TCCTGAAGAG 
CCCAACGGAC AGATTCGAGG ATATCGAGTG TACTACACGT CTGACCTGCA CTTCCCGCTC 
AGCACATGGC AGAAGCACAA CACAGAGGAC AGCAGTCTGA CCACCATCTC CGGCCTGGTT 
CCTGACATCA CGTATGGGCT CCGTGTGCTG GCCTTCACCT CTGTTGGAGA TGGACCACCT 
TCCGATATAC TGCAGGTTAA AACACAGCAA GGAGTCCCTG CTCAGCCGAC TGGTTTTGAA 
GCTGAAGCCG AGTTGGACAC GCGGATCATG CTGTCATGGT TGTGGCCCGT CCAAGATCAG 
ATCACCAAAT ATGAGCTCAC ATACTGGGAG GCTGACTCTG ACAACAAGCA TCATGTCATC 
TTTGACCCGG CCGGTTCATA TGCTATTGAC AGCCTGAAAC CAGACACTCT CTATAAATTC 
TCTCTGGCGG CGCGCTCTGA GATGGGGCTC GGGGTTTACA CACAGCCTAT CGAGGCCCGC 
ACAGCCCAGT CCGCCCCGTC CGCTCCCCCA CAAGAGGTGC ACCTGATCAG CCTGAGCTCC 
AGCAGCATCA AGGTGAGTTG GGTGGCCCCT CCGGCCGCCA GCCGGCACGG CAGCATCGTC 
CGCTACTCTC TGGCCTATCA GGCAGTGTCT GGCGAGGACC AGGAGCGCCA CGAGGTCAAA 
GACATCCCTG CTGATGTGTC CAGCTATGTT CTGGAGGGTC TGGAGAAATG GACTGAGTAT 
ACGGTGTGGG TCAAAGCGCA CACAGATGTG GGTCCCGGGC CAGAAAGTGG CAGCACCCGC 
ATCAGAACCC AAGAGGATGT GCCTGGTGCT CCTCCACGCA GGGTTGAGGT GGACAACATC 
AACTCCACTG CACTGCGTGT GTCCTGGAAG CCGCCTCTGC AGCAGAAACA ACACGGCCAC 
ATACGTGGAT ATCAGGTGGT TTACTCACGC CTGGAGAATG GAGAGCCGCG CGGACAGCCC 
ATCATTCTGG ACGTGGCGCT GCCAGAGGCA CAGGAGGCCG TGATCAGTGG TCTTCTCCCT 
GAAACCACAT ATTCAGTGAC TGTAGCCGCG TACACCACTA AAGGAGATGG AGCGCGGAGC 
AAAGCCAAAG TGGTGACCAC AACAGGAGCA GTTCCAGGAA AGCCCACTAT GATCATCAGC 
ACCACCATAG GCAACACGGC TCTGATCCAG TGGCAGCCGC CTAAAGAGAT GGTGGGAGAG 
CTGATGGGCT ACAGACTCCG CTATAAGCGT GAAGAAGAGG ATATTTACAC TGTCCGAGAC 
TTCAGACGCA CTGATGACCA CTTCACTGTC ACCGGCCTGC ACAAGGGCGC GACGTACATC 
TTCAAACTCT GCGCTAAAAA CCGCGCCGGG AATGGAGAGG AGTACAGCAA AGAGATCAGC 
ACCCCGGAGG ATGTGCCCAG CAGTTACCCA CAAAACCTCA AAGTCGTTGG ATTGACCACA 
ACCACCACAA AACTAGCCTG GGATCCTCCG CCGCTGCCCG AGAGGAATGG GAAGATCGTA 
CGATATGTGG TGGTTTATCG AGACATTAAC AGCCATCAGA ATCAGACCAA CGGCACAGCG 
GACACGGAGA TGACCATTCA GGGCCTGAAG CCGGACACCA CCTATGATAT CAGGGTGAGG 
GCGTTTACAG GGAAAGGTGG AGGTCCCATC AGCCCCAGCA TCCAGAGCAG AACCATGTCT 
ACCTCCATGC CCGAGTTCAC GAAAAACTTT GGTGTGAAAG CCGTGATGAA GACCTCTGTC 
CTGCTCACAT GGGAGGTGCC AGAAACATAC AAGTCTCAAG TGCCTTTCAA GATCCTGTAT 
AACCAGCAGA GTGTGGAGGT CCAGGGTGAC CTGAAGAGGA AGCTGATTAC GCGTCTGCAG 
CCGGACACTG ATTACTCCTT TGTCCTGATG AGTCGGGGGA ACAGCGCTGG CGGACTGCAG 
CAGCAGGTCT CCATCCGCAC CGCTCCAGAC CTGTTCACTA CCAAACCAGC ACACCAGAAA 
CTAGACCCAG AGGAGGGCGG CAAACTCGCC ATCACACTGC CCGAAGTGCA CAACAGCGCC 
CTCGTCAGGT GGTTTTATAT CGTGGTTGTA CCAGTAACTC CTTCAACTCT CCGCCATTGG 
GAAAACCCAG AAGATATTGA TCTGGATGAG CTGATGGGGA ATAATAGAGA CGCAGCTGTG 
AGGAGGAGGA GAAGACAAAC TCAGGACTTT CTGCGGCCTT ACATTGCTGC CAAGCTGGAG 
TCTCTTCCAG AAAGATTTGT GTTGGGAGAT GAGAAAACCT ACAGCAACTA CTACAACAAA 
CCTCTGCCGG GACAGCAGCA GTATCGCACA TTTATACTGG CGGCACTGAA AGACAGAGAC 
TCGACGTTCA CAGTGAGTCC GTTCTCTGAG CCCATGATGG TGAAGCTCCA CAGCGGGATG 
TCCCGTCACG TGGAGGACCC GGAGATGCTG TGGGTGATGG GACCAGTGTT AGCCGTGGTC 
CTCATCATCA TCATCGTCAT CGCCATCCTG CTCTTCAAGA GGAAGCGCAC GTCTCCCTCA 
GCTAAAGATG AGCACTCAGC TGGAGTTAAA GATCACCTGC TGGCTCATTC GTCAGATCCA 
GTGGAGATGA GGAGGCTCAA CTACCAGACA CAAGGAATGC GGGAACACCC TCCAGTGTCT 
GTGTGTGCTT TGGCTGACCA CATCGAGCGG CTGAGAGCCA ACGACAGCCT CCGCTTCTCG 
CAGGAGTATG AGTCTGTTGA CCCTGGCCAG CAGTTCACCT GGGAAAACTC CAACTTAGAG 
GTCAACAAGC CCAAGAACCG CTACGCAAAC GTCATCGCTT ACGATCACTC CAGGGTTGTG 
CTGACTCCTG TGGATGGTGT TCCCGGAAGT GACTACATCA ATGGAAACTA CATTGATGGC 
TACCGTAAGC AGAACGCCTA CATCGCCACG CAGGGTCCGC TGCCTGAGAC GCTCAGTGAC 
TTCTGGAGGA TGGTGTGGGA ACAGAGAACC AGCACTATCG TCATGATGAC CCGACTGGAG 
GAGAAGAGCA GGGTGAAGTG TGATCAGTAC TGGCCAAGCC GAGGAACAGA GACTTACGGG 
ATGATTCAAG TGTCCATGCT GGATACGGTG GAGCTGGCCA CATACAGCGT CAGAACCTTC 
GCTCTCTACA AGAATGGATC CAGCGAGAAA CGGGAGGTTC GGCAGTTCCA GTTCATGGCG 
TGGCCGGATC ACGGTGTTCC CGAATATCCC ACACCCATTT TAGCCTTCTT ACGCAGGGTC 
AAAGCCTGCA ACCCACCTGA CGCGGGACCC ATGGTGGTGC ACTGCAGCGC TGGCGTGGGT 
CGGACGGGCT GTTTCATCGT AATAGACGCC ATGCTGGAGC GGATGAAGCA CGAGAAGTCG 
GTGGACATCT ACGGTCACGT GACCTGCATG CGTTCTCAGA GGAACTACAT GGTGCAGACA 
GAGGATCAGT ACATTTTCAT TCACGAGGCC CTGCTGGAGG CTGCCACCTG CGGGAACACA 
GAGGTGCCGG CGCGGAGCCT CTACACACAC ATCCAGAAAC TCACACAGGC GCCGCCTGGA 
GACACCGTCA CCGCCATGGA GCTCGAATTT AAGAAACTGG CCAACTCTAA AGCACACACC 
TCCAGATTCA TCAGCGCTAG TCTGCCCTGC AACAAATTCA AGAACCGGCT GGTGAACATC 
ATGCCTTTCG AGTCCAGCCG TGTTTGTCTG CAGCCAATCA GAGGCGTGGA GGGCTCCGAC 
TACATCAACG CCAGCTTCAT CGACGGATAC AGGCAGCAGA AGGCATATAT CGCCACTCAA 
GGCCCGCTGT CTGAAACCAC AGAGGACTTC TGGAGGATGC TTTGGGAGCA CAACTCCACC 
ATCGTGGTGA TGCTGACGAA GCTTAGAGAG ATGGGACGGG AGAAATGTCA TCAGTACTGG 
CCTGCAGAGA GATCTGCGCG CTACCAGTAT TTCGTTGTGG ATCCTATGGC TGAATATAAC 
ATGCCACAGT ACATCTTACG GGAGTTTAAA GTCACAGACG CCAGAGATGG CCAGTCTCGG 
ACTATCCGAC AGTTTCAGTT CACAGATTGG CCGGAACAAG GTGTGCCAAA GACAGGAGAG 
GGCTTTATTG ATTTTATTGG TCAAGTCCAC AAAACCAAGG AGCAGTTTGG ACAGGACGGA 
CCAATTACTG TTCACTGCAG TGCCGGTGTG GGCAGAACAG GCGTCTTCAT CACTTTAAGT 
ATTGTTCTGG AGCGGATGAG GTATGAAGGA GTGGTGGACA TGTTCCAGAC CATAAAAACA 
CTCCGCACGC AGAGACCTGC CATGGTACAG ACCGAGGACC AGTACCAGCT GTGCTACAGA 
GCAGCTCTGG AGTACCTGGG CAGCTTCGAT CACTATGCAA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001298277.1
CDS	308..5992
Translation	MVPQTRQGSARHGATSLLLLLLCLAVPIHADSMPSFVKTPEDQTGISGGVASFVCQAVGEPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRTHRDEAIYECTATNSVGEINTSAKLTVLEEDQIPHGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEISWFKDFLPVDINSSNGRIKQLRSGALQIENSEESDQGKYECVAVNSAGTRYSAPANLYVRVRRVPPRFSIPPTNHEVMPGGSVNLTCVAVGAPMPYVKWMKGEQELTKEEEMPIGRNVLELTNIRQSGNYTCVAISSLGMIDTTAQITVKALPRPPASLIVTETTATSVTLTWDSGNPEPVSYYVIQYRSKISDNGFQEVDGVASTRYSIGGLSPYSEYEFRVMAVNNIGRGPPSGMVETRTSEQAPSSPPLRVQARMLSATTMLVQWEPPEEPNGQIRGYRVYYTSDLHFPLSTWQKHNTEDSSLTTISGLVPDITYGLRVLAFTSVGDGPPSDILQVKTQQGVPAQPTGFEAEAELDTRIMLSWLWPVQDQITKYELTYWEADSDNKHHVIFDPAGSYAIDSLKPDTLYKFSLAARSEMGLGVYTQPIEARTAQSAPSAPPQEVHLISLSSSSIKVSWVAPPAASRHGSIVRYSLAYQAVSGEDQERHEVKDIPADVSSYVLEGLEKWTEYTVWVKAHTDVGPGPESGSTRIRTQEDVPGAPPRRVEVDNINSTALRVSWKPPLQQKQHGHIRGYQVVYSRLENGEPRGQPIILDVALPEAQEAVISGLLPETTYSVTVAAYTTKGDGARSKAKVVTTTGAVPGKPTMIISTTIGNTALIQWQPPKEMVGELMGYRLRYKREEEDIYTVRDFRRTDDHFTVTGLHKGATYIFKLCAKNRAGNGEEYSKEISTPEDVPSSYPQNLKVVGLTTTTTKLAWDPPPLPERNGKIVRYVVVYRDINSHQNQTNGTADTEMTIQGLKPDTTYDIRVRAFTGKGGGPISPSIQSRTMSTSMPEFTKNFGVKAVMKTSVLLTWEVPETYKSQVPFKILYNQQSVEVQGDLKRKLITRLQPDTDYSFVLMSRGNSAGGLQQQVSIRTAPDLFTTKPAHQKLDPEEGGKLAITLPEVHNSALVRWFYIVVVPVTPSTLRHWENPEDIDLDELMGNNRDAAVRRRRRQTQDFLRPYIAAKLESLPERFVLGDEKTYSNYYNKPLPGQQQYRTFILAALKDRDSTFTVSPFSEPMMVKLHSGMSRHVEDPEMLWVMGPVLAVVLIIIIVIAILLFKRKRTSPSAKDEHSAGVKDHLLAHSSDPVEMRRLNYQTQGMREHPPVSVCALADHIERLRANDSLRFSQEYESVDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVVLTPVDGVPGSDYINGNYIDGYRKQNAYIATQGPLPETLSDFWRMVWEQRTSTIVMMTRLEEKSRVKCDQYWPSRGTETYGMIQVSMLDTVELATYSVRTFALYKNGSSEKREVRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKSVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAATCGNTEVPARSLYTHIQKLTQAPPGDTVTAMELEFKKLANSKAHTSRFISASLPCNKFKNRLVNIMPFESSRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLSETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTIKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	NM_001311348.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase receptor type Fa (ptprfa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001311348.1

ORF Insert Sequence:

ATGGTGCCAC AAACCCGACA GGGCTCCGCT CGCCACGGTG CCACGTCCCT ACTGCTGCTG 
CTGCTCTGCC TGGCGGTGCC AATCCACGCA GACAGTATGC CCAGTTTCGT TAAGACTCCT 
GAAGATCAGA CAGGGATTTC AGGAGGGGTG GCATCATTCG TGTGCCAGGC GGTCGGAGAA 
CCCAAACCTC GCATTACCTG GATGAAGAAG GGCAAGAAAG TCAGCTCTCA GCGCTTCGAG 
GTAATAGAGT TTGATGACGG CTCAGGCTCT GTGCTTCGGA TCCAGCCATT ACGGACACAT 
CGAGATGAGG CGATTTACGA GTGCACAGCT ACCAACAGTG TTGGAGAAAT TAACACTAGT 
GCAAAACTCA CTGTATTAGA AGAGGACCAG ATCCCTCATG GTTTCCCCAC TATAGACATG 
GGTCCCCAGC TGAAAGTGGT TGAACGGACG CGGACGGCCA CCATGCTCTG TGCCGCCAGC 
GGAAACCCAG ACCCGGAGAT CTCCTGGTTC AAAGACTTTC TGCCTGTCGA CATCAACAGC 
AGCAACGGAC GAATCAAGCA GCTCCGTTCA GGAGCGCTGC AGATCGAGAA CAGCGAGGAA 
TCAGACCAGG GCAAGTATGA ATGTGTGGCG GTGAACAGCG CGGGAACTCG ATACTCGGCT 
CCTGCAAACC TCTACGTCAG AGTCCGACGG GTTCCCCCAC GTTTTTCAAT CCCTCCAACC 
AATCACGAGG TGATGCCGGG TGGCAGTGTG AACCTCACCT GCGTGGCAGT GGGCGCTCCA 
ATGCCCTATG TGAAGTGGAT GAAGGGCGAA CAGGAGCTGA CCAAAGAAGA GGAGATGCCC 
ATTGGCCGAA ATGTGCTGGA GCTCACCAAC ATCCGCCAGT CGGGGAACTA CACCTGCGTG 
GCCATATCAT CACTGGGCAT GATCGACACC ACAGCGCAGA TCACTGTTAA AGCTCTTCCT 
CGTCCTCCAG CCTCTCTGAT TGTCACTGAA ACCACCGCCA CCAGTGTGAC TTTAACATGG 
GATTCTGGCA ACCCAGAACC CGTATCATAT TACGTGATCC AGTATCGATC CAAAATCTCT 
GACAATGGTT TCCAGGAGGT GGACGGCGTG GCATCCACCC GCTACAGCAT CGGCGGGTTG 
AGTCCGTACT CAGAGTATGA GTTTCGAGTA ATGGCGGTCA ATAACATTGG TCGCGGGCCG 
CCCAGCGGGA TGGTGGAGAC TCGTACCAGT GAACAGGCAC CGTCATCTCC TCCGCTGCGC 
GTTCAAGCCC GGATGTTGAG CGCTACGACT ATGCTGGTGC AATGGGAACC TCCTGAAGAG 
CCCAACGGAC AGATTCGAGG ATATCGAGTG TACTACACGT CTGACCTGCA CTTCCCGCTC 
AGCACATGGC AGAAGCACAA CACAGAGGAC AGCAGTCTGA CCACCATCTC CGGCCTGGTT 
CCTGACATCA CGTATGGGCT CCGTGTGCTG GCCTTCACCT CTGTTGGAGA TGGACCACCT 
TCCGATATAC TGCAGGTTAA AACACAGCAA GGAGTCCCTG CTCAGCCGAC TGGTTTTGAA 
GCTGAAGCCG AGTTGGACAC GCGGATCATG CTGTCATGGT TGTGGCCCGT CCAAGATCAG 
ATCACCAAAT ATGAGCTCAC ATACTGGGAG GCTGACTCTG ACAACAAGCA TCATGTCATC 
TTTGACCCGG CCGGTTCATA TGCTATTGAC AGCCTGAAAC CAGACACTCT CTATAAATTC 
TCTCTGGCGG CGCGCTCTGA GATGGGGCTC GGGGTTTACA CACAGCCTAT CGAGGCCCGC 
ACAGCCCAGT CCGCCCCGTC CGCTCCCCCA CAAGAGGTGC ACCTGATCAG CCTGAGCTCC 
AGCAGCATCA AGGTGAGTTG GGTGGCCCCT CCGGCCGCCA GCCGGCACGG CAGCATCGTC 
CGCTACTCTC TGGCCTATCA GGCAGTGTCT GGCGAGGACC AGGAGCGCCA CGAGGTCAAA 
GACATCCCTG CTGATGTGTC CAGCTATGTT CTGGAGGGTC TGGAGAAATG GACTGAGTAT 
ACGGTGTGGG TCAAAGCGCA CACAGATGTG GGTCCCGGGC CAGAAAGTGG CAGCACCCGC 
ATCAGAACCC AAGAGGATGT GCCTGGTGCT CCTCCACGCA GGGTTGAGGT GGACAACATC 
AACTCCACTG CACTGCGTGT GTCCTGGAAG CCGCCTCTGC AGCAGAAACA ACACGGCCAC 
ATACGTGGAT ATCAGGTGGT TTACTCACGC CTGGAGAATG GAGAGCCGCG CGGACAGCCC 
ATCATTCTGG ACGTGGCGCT GCCAGAGGCA CAGGAGGCCG TGATCAGTGG TCTTCTCCCT 
GAAACCACAT ATTCAGTGAC TGTAGCCGCG TACACCACTA AAGGAGATGG AGCGCGGAGC 
AAAGCCAAAG TGGTGACCAC AACAGGAGCA GTTCCAGGAA AGCCCACTAT GATCATCAGC 
ACCACCATAG GCAACACGGC TCTGATCCAG TGGCAGCCGC CTAAAGAGAT GGTGGGAGAG 
CTGATGGGCT ACAGACTCCG CTATAAGCGT GAAGAAGAGG ATATTTACAC TGTCCGAGAC 
TTCAGACGCA CTGATGACCA CTTCACTGTC ACCGGCCTGC ACAAGGGCGC GACGTACATC 
TTCAAACTCT GCGCTAAAAA CCGCGCCGGG AATGGAGAGG AGTACAGCAA AGAGATCAGC 
ACCCCGGAGG ATGTGCCCAG CAGTTACCCA CAAAACCTCA AAGTCGTTGG ATTGACCACA 
ACCACCACAA AACTAGCCTG GGATCCTCCG CCGCTGCCCG AGAGGAATGG GAAGATCGTA 
CGATATGTGG TGGTTTATCG AGACATTAAC AGCCATCAGA ATCAGACCAA CGGCACAGCG 
GACACGGAGA TGACCATTCA GGGCCTGAAG CCGGACACCA CCTATGATAT CAGGGTGAGG 
GCGTTTACAG GGAAAGGTGG AGGTCCCATC AGCCCCAGCA TCCAGAGCAG AACCATGTCT 
ACCTCCATGC CCGAGTTCAC GAAAAACTTT GGTGTGAAAG CCGTGATGAA GACCTCTGTC 
CTGCTCACAT GGGAGGTGCC AGAAACATAC AAGTCTCAAG TGCCTTTCAA GATCCTGTAT 
AACCAGCAGA GTGTGGAGGT CCAGGGTGAC CTGAAGAGGA AGCTGATTAC GCGTCTGCAG 
CCGGACACTG ATTACTCCTT TGTCCTGATG AGTCGGGGGA ACAGCGCTGG CGGACTGCAG 
CAGCAGGTCT CCATCCGCAC CGCTCCAGAC CTGTTCACTA CCAAACCAGC ACACCAGAAA 
CTAGACCCAG AGGAGGGCGG CAAACTCGCC ATCACACTGC CCGAAGTGCA CAACAGCGCC 
CTCGTCAGGT GGTTTTATAT CGTGGTTGTA CCAGTAACTC CTTCAACTCT CCGCCATTGG 
GAAAACCCAG AAGATATTGA TCTGGATGAG CTGATGGGGA ATAATAGAGA CGCAGCTGTG 
AGGAGGAGGA GAAGACAAAC TCAGGACTTT CTGCGGCCTT ACATTGCTGC CAAGCTGGAG 
TCTCTTCCAG AAAGATTTGT GTTGGGAGAT GAGAAAACCT ACAGCAACTA CTACAACAAA 
CCTCTGCCGG GACAGCAGCA GTATCGCACA TTTATACTGG CGGCACTGAA AGACAGAGAC 
TCGACGTTCA CAGTGAGTCC GTTCTCTGAG CCCATGATGG TGAAGCTCCA CAGCGGGATG 
TCCCGTCACG TGGAGGACCC GGAGATGCTG TGGGTGATGG GACCAGTGTT AGCCGTGGTC 
CTCATCATCA TCATCGTCAT CGCCATCCTG CTCTTCAAGA GGAAGCGCAC GTCTCCCTCA 
GCTAAAGATG AGCACTCAGC TGGAGTTAAA GATCACCTGC TGGCTCATTC GTCAGATCCA 
GTGGAGATGA GGAGGCTCAA CTACCAGACA CAAGGAATGC GGGAACACCC TCCAGTGTCT 
GTGTGTGCTT TGGCTGACCA CATCGAGCGG CTGAGAGCCA ACGACAGCCT CCGCTTCTCG 
CAGGAGTATG AGTCTGTTGA CCCTGGCCAG CAGTTCACCT GGGAAAACTC CAACTTAGAG 
GTCAACAAGC CCAAGAACCG CTACGCAAAC GTCATCGCTT ACGATCACTC CAGGGTTGTG 
CTGACTCCTG TGGATGGTGT TCCCGGAAGT GACTACATCA ATGGAAACTA CATTGATGGC 
TACCGTAAGC AGAACGCCTA CATCGCCACG CAGGGTCCGC TGCCTGAGAC GCTCAGTGAC 
TTCTGGAGGA TGGTGTGGGA ACAGAGAACC AGCACTATCG TCATGATGAC CCGACTGGAG 
GAGAAGAGCA GGGTGAAGTG TGATCAGTAC TGGCCAAGCC GAGGAACAGA GACTTACGGG 
ATGATTCAAG TGTCCATGCT GGATACGGTG GAGCTGGCCA CATACAGCGT CAGAACCTTC 
GCTCTCTACA AGAATGGATC CAGCGAGAAA CGGGAGGTTC GGCAGTTCCA GTTCATGGCG 
TGGCCGGATC ACGGTGTTCC CGAATATCCC ACACCCATTT TAGCCTTCTT ACGCAGGGTC 
AAAGCCTGCA ACCCACCTGA CGCGGGACCC ATGGTGGTGC ACTGCAGCGC TGGCGTGGGT 
CGGACGGGCT GTTTCATCGT AATAGACGCC ATGCTGGAGC GGATGAAGCA CGAGAAGTCG 
GTGGACATCT ACGGTCACGT GACCTGCATG CGTTCTCAGA GGAACTACAT GGTGCAGACA 
GAGGATCAGT ACATTTTCAT TCACGAGGCC CTGCTGGAGG CTGCCACCTG CGGGAACACA 
GAGGTGCCGG CGCGGAGCCT CTACACACAC ATCCAGAAAC TCACACAGGC GCCGCCTGGA 
GACACCGTCA CCGCCATGGA GCTCGAATTT AAGAAACTGG CCAACTCTAA AGCACACACC 
TCCAGATTCA TCAGCGCTAG TCTGCCCTGC AACAAATTCA AGAACCGGCT GGTGAACATC 
ATGCCTTTCG AGTCCAGCCG TGTTTGTCTG CAGCCAATCA GAGGCGTGGA GGGCTCCGAC 
TACATCAACG CCAGCTTCAT CGACGGATAC AGGCAGCAGA AGGCATATAT CGCCACTCAA 
GGCCCGCTGT CTGAAACCAC AGAGGACTTC TGGAGGATGC TTTGGGAGCA CAACTCCACC 
ATCGTGGTGA TGCTGACGAA GCTTAGAGAG ATGGGACGGG AGAAATGTCA TCAGTACTGG 
CCTGCAGAGA GATCTGCGCG CTACCAGTAT TTCGTTGTGG ATCCTATGGC TGAATATAAC 
ATGCCACAGT ACATCTTACG GGAGTTTAAA GTCACAGACG CCAGAGATGG CCAGTCTCGG 
ACTATCCGAC AGTTTCAGTT CACAGATTGG CCGGAACAAG GTGTGCCAAA GACAGGAGAG 
GGCTTTATTG ATTTTATTGG TCAAGTCCAC AAAACCAAGG AGCAGTTTGG ACAGGACGGA 
CCAATTACTG TTCACTGCAG TGCCGGTGTG GGCAGAACAG GCGTCTTCAT CACTTTAAGT 
ATTGTTCTGG AGCGGATGAG GTATGAAGGA GTGGTGGACA TGTTCCAGAC CATAAAAACA 
CTCCGCACGC AGAGACCTGC CATGGTACAG ACCGAGGACC AGTACCAGCT GTGCTACAGA 
GCAGCTCTGG AGTACCTGGG CAGCTTCGAT CACTATGCAA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ptprfa
Entrez Gene ID	140819
Full Name	protein tyrosine phosphatase, receptor type, f, a
Synonyms	LAR,ptprf
General protein information	Preferred Names protein tyrosine phosphatase, receptor type, f, a Names receptor-type tyrosine-protein phosphatase F zf-LAR
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Rattus norvegicus (Norway rat)	Ptprf	NP_062122.1
Danio rerio (zebrafish)	ptprfb	NP_001077045.1
Danio rerio (zebrafish)	ptprfa	XP_692206.6
	PTPRF	XP_001095521.2
Pan troglodytes (chimpanzee)	PTPRF	XP_001173898.2
Canis lupus familiaris (dog)	PTPRF	XP_005629167.1
Gallus gallus (chicken)	PTPRF	XP_004936750.1
Homo sapiens (human)	PTPRF	NP_002831.2
Mus musculus (house mouse)	Ptprf	NP_035343.2
Bos taurus (cattle)	PTPRF	NP_001094549.1
Xenopus tropicalis (tropical clawed frog)	ptprf	XP_004914019.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ptprfa cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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