ptprf gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_018093545.1	XP_017949034.1	receptor-type tyrosine-protein phosphatase F isoform X6
XM_031900926.1	XP_031756786.1	receptor-type tyrosine-protein phosphatase F isoform X4
XM_018093546.1	XP_017949035.1	receptor-type tyrosine-protein phosphatase F isoform X7
XM_031900930.1	XP_031756790.1	receptor-type tyrosine-protein phosphatase F isoform X9
XM_012961577.1	XP_012817031.1	receptor-type tyrosine-protein phosphatase F isoform X1
XM_031900923.1	XP_031756783.1	receptor-type tyrosine-protein phosphatase F isoform X1
XM_031900928.1	XP_031756788.1	receptor-type tyrosine-protein phosphatase F isoform X6
XM_031900929.1	XP_031756789.1	receptor-type tyrosine-protein phosphatase F isoform X7
XM_004913963.2	XP_004914020.2	receptor-type tyrosine-protein phosphatase F isoform X10
XM_031900931.1	XP_031756791.1	receptor-type tyrosine-protein phosphatase F isoform X11
XM_031900932.1	XP_031756792.1	receptor-type tyrosine-protein phosphatase F isoform X12
XM_031900936.1	XP_031756796.1	receptor-type tyrosine-protein phosphatase F isoform X16
XM_004913961.1	XP_004914018.1	receptor-type tyrosine-protein phosphatase F isoform X2
XM_018093543.1	XP_017949032.1	receptor-type tyrosine-protein phosphatase F isoform X3
XM_031900924.1	XP_031756784.1	receptor-type tyrosine-protein phosphatase F isoform X2
XM_031900925.1	XP_031756785.1	receptor-type tyrosine-protein phosphatase F isoform X3
XM_031900934.1	XP_031756794.1	receptor-type tyrosine-protein phosphatase F isoform X14
XM_004913963.1	XP_004914020.1	receptor-type tyrosine-protein phosphatase F isoform X4
XM_018093544.1	XP_017949033.1	receptor-type tyrosine-protein phosphatase F isoform X5
XM_031900922.1	XP_031756782.1	receptor-type tyrosine-protein phosphatase F isoform X1
XM_031900927.1	XP_031756787.1	receptor-type tyrosine-protein phosphatase F isoform X5
XM_004913961.2	XP_004914018.2	receptor-type tyrosine-protein phosphatase F isoform X8
XM_031900933.1	XP_031756793.1	receptor-type tyrosine-protein phosphatase F isoform X13
XM_031900935.1	XP_031756795.1	receptor-type tyrosine-protein phosphatase F isoform X15

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa44150	XM_018093543.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa44151	XM_018093544.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa44152	XM_018093545.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa44153	XM_018093546.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa57128	XM_031900923.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57128	XM_031900922.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57129	XM_031900924.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57130	XM_031900925.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57131	XM_031900926.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57132	XM_031900927.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57133	XM_031900928.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57134	XM_031900929.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57135	XM_004913961.2 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57136	XM_031900930.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57137	XM_004913963.2 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57138	XM_031900931.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57139	XM_031900932.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57140	XM_031900933.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57141	XM_031900934.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57142	XM_031900935.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57143	XM_031900936.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa09448	XM_004913961.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa09450	XM_004913963.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa35597	XM_012961577.1 Latest version!	Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ptprf ( XM_018093543.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949032.1 Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa44150
Clone ID Related Accession (Same CDS sequence)	XM_018093543.1
Accession Version	XM_018093543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTCAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACATT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGAGATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCAAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTAACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTGCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA AGATGCTGTT ATCACTGGCC TCTCTCCTGA GACCACTTAT 
TCCATCACTG TGGCCGCCTA CACTACTAAG GGAGATGGTG CTAGAAGCAA ACCCAAGGTC 
GTTAGCACCA CAGGAGCTGT GCCTGGTAAA CCCACTATGA TGATCAGCAC CACAGCACTG 
AACACTGCTC TGATCCAGTG GCATCCTCCA AGAGAGATTG TGGGAGAGTT GTTAGGATAT 
CGTTTGCAGT ACAAGCGAGT GGATGAAGAC AGGTTTATTA CAATTGATTT TAGCAAGAAT 
GAACATCACC ACACAGTGAC TGGGCTACAT AAAGGTGCAA GCTATATATT CAAGCTTTCT 
GCAAAAAACC GAGCTGGAGC AGGGGAGGAA TATGAAAAGG AAATTTCCAC AGCTGAAGAT 
TTTCCTAGTG GGTTTCCCCA AAATCTGCAA GTAGTTGGAC TTACAACCTC TACTACAGAA 
GTGTCCTGGG AACCACCAGT GTTGTCTGAG CGCAATGGAA AGATCACCAA CTACACAGTG 
GTGTATCGGG ACATAAATAG CCAGCAGGAT CTCACCAATG TCACTCGCGA CACACACATA 
ACATTGGTCA ATCTCAAGCC AGACACCACA TATGACATCA AGGTCAGGGC AAGAACAAAC 
AAAGGCGCTG GCCCTCTAAG TCCTAGCATT CAATCACGTA CAATGCCTGT TGACCAAGTG 
TTTGCTAAGA ATTTCAGAGT AAACGCCGTG ACAAAAACCT CAGTCTTGCT AAGCTGGGAG 
CTGCCTGATT CTTACAAATC AGCTGTGCCT TTCAAGATCT TTTACAATAA TCAACATGTA 
GAAGTTGATG GACACTCTAT GCGCAAACTG ATCAGTAATC TCAAGCCAGA TACAGAATAC 
TCCTTTGTGC TGATGAACAG GGGGAGCAGT GCAGGTGGAC TGCAGCACCG CGTGTCCATT 
CGCACAGCAC CAGATGTTTT AAAAAGCAAG CCTTTTGCTG CCAACAAATA CATGGAGGAG 
GGCAGGTTCA CCTTGCTGTT GCCTCAGGTT CAGACTACTT CCCCAGTAAG GTGGTATTAT 
ATTGTGGTTG TCCCACTGGA CAGTTCCAGC GGGGGTCTGA ATTCTCGCTG GCAGAGCCCT 
GAGGAAATGG AGCTAGAGCA GATACTTGAA GCAATAAACC AGGGATCCCG TCGTCATAGA 
AGACAGGCAA GCAAGAATAA GCCATACATT GCAGCCCGAG TAAACACACT TCCAGAATCC 
TTTATTTTGG GTGACAATAA AGAATATAAT GGGTTCATCA ACAAGCCACT GAATCAGAAT 
TTACGCTACA CCTGCTTTGT GCTAGCTGCA CTAGAAGATG GGGACACTAA AAAGTATGCA 
GCCAGCCCAT ACTCAGATGA TATTGTGGTG GAGGTGATCT CACAGGCAAA ACAACAGGAA 
GAGCCAGAAA TGCTTTGGGT CATGGGTCCT GTTCTGGCTG TAATTCTTAT AATAATTATA 
GTTATTGCCA TTCTACTTTT CAAAAGTAAA CAGGAAAGGA AAAGGACCAA CTCTCCTTCA 
GGCAAAGATG AACAGTCAGT AGGACTTAAA GACTGTCTCC TCGCTCACTC CTCAGATCCA 
GTTGAAATGA GGCGTCTAAA CTACCAAACT CCAGGTATGA GAGACCACCC TCCTATTCCA 
ATCTCTGACC TGGCAGACAA CATTGAAAGA CTGAAGGCTA ATGATGGACT CAAGTTCTCT 
CAGGAATATG AGTCTATTGA TCCAGGTCAG CAATTCACGT GGGAAAATTC AAATCTAGAA 
GTAAACAAAC CAAAGAATCG CTATGCAAAT GTCATTGCAT ATGACCATTC AAGGGTCATT 
CTTACCTCAG TGGATGGAGT TCCCGGAAGT GATTACATTA ATGCCAATTA CATAGATGGG 
TACAGGAAAC AGAATGCCTA TATAGCTACG CAGGGCCCAC TGCCAGAGAC TATCACTGAT 
TTCTGGAGAA TGGTGTGGGA GCAAAGAACC GCCACTATTG TCATGATGAC CCGGCTAGAG 
GAAAAATCAA GGGTGAAATG TGACCAGTAC TGGCCCGGTC GTGGCTCAGA GACCTATGGT 
ATGATCCAAG TCACATTAAT GGACACAGTA GAGCTTGCCA CCTACACAGT TAGGACTTTC 
ACACTTTATA AGAATGGCTC CAGTGAGAAG CGGGATGTCA GACAGTTCCA ATTTATGGCC 
TGGCCTGATC ACGGGGTCCC TGAATATCCT ACTCCTATTC TTGGATTCCT TCGCAGAGTG 
AAAGGCTGTA ATCCACCTGA TGCTGGTCCT ATGGTTGTTC ATTGCAGTGC TGGTGTGGGC 
AGAACAGGAT GCTTTATTGT TATTGATGCC ATGCTAGAAC GGGTGAAACT GGAGAAAACT 
GTGGATATTT ATGGTCATGT GACCTGCATG CGCTCCCAAC GAAATTATAT GGTGCAAACA 
GAGGACCAGT ACATCTTTAT CCATGAGGCT TTGCTTGAAG CAGTAATGTG CGGGATCACT 
GAAGTGCCAG CACGCAGTCT GTATGCACAT ATCCAGAAAC TGTCTCAGGT CCCTCCGGGT 
GAGAGTGTTA CCTCCATGGA ACTAGAGTTC AAGCTTTTGG CCAACTCTAA AGCCCACACC 
TCACGATTTA TCAGTGCCAA CCTCCCATGC AACAAGTTCA AAAACAGACT GGTAAACATC 
ATGCCATATG AACTGAGTCG TGTCTCCCTG CAGCCCATTC GTGGTGTTGA GGGATCAGAT 
TATATCAATG CAAGTTTCAT TGATGGCTAC AGGCAGCAGA AAGGCTACAT TGCTACTCAG 
GGTCCATTGG CCGAGACCAC TGAGGATTTC TGGAGAATGC TGTGGGAACA TAACTCCACC 
ATTGTGGTGA TGCTGACAAA GTTGCGAGAA ATGGGGAGGG AGAAGTGCCA TCAGTACTGG 
CCTGCAGAGC GCTCAGCACG GTACCAGTAT TTTGTGGTGG ACCCTATGGC TGAGTATAAC 
ATGCCTCAGT ATATCCTGCG TGAGTTCAAA GTGACAGATG CACGGGATGG CCAATCTCGT 
ACAATTAGAC AATTCCAGTT CACTGATTGG CCTGAACAAG GAGTGCCAAA AACAGGTGAA 
GGCTTTATTG ACTTCATTGG GCAGGTGCAC AAAACCAAGG AGCAGTTTGG GCAGGATGGG 
CCAATCACCG TTCACTGCAG CGCTGGCGTG GGGCGCACTG GAGTATTCAT CACTCTCAGT 
ATTGTACTGG AGCGTATGAG GTACGAGGGT GTGGTTGACA TGTTTCAAAC AGTGAAGACT 
TTAAGAACAC AGCGGCCAGC AATGGTACAA ACAGAGGACC AGTATCAACT GTGTTATAGA 
GCCGCACTGG AGTATCTGGG CAGCTTTGAC CACTATGCAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949032.1
CDS	635..6319
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSEIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKSKQERKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_018093543.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093543.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTCAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACATT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGAGATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCAAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTAACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTGCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA AGATGCTGTT ATCACTGGCC TCTCTCCTGA GACCACTTAT 
TCCATCACTG TGGCCGCCTA CACTACTAAG GGAGATGGTG CTAGAAGCAA ACCCAAGGTC 
GTTAGCACCA CAGGAGCTGT GCCTGGTAAA CCCACTATGA TGATCAGCAC CACAGCACTG 
AACACTGCTC TGATCCAGTG GCATCCTCCA AGAGAGATTG TGGGAGAGTT GTTAGGATAT 
CGTTTGCAGT ACAAGCGAGT GGATGAAGAC AGGTTTATTA CAATTGATTT TAGCAAGAAT 
GAACATCACC ACACAGTGAC TGGGCTACAT AAAGGTGCAA GCTATATATT CAAGCTTTCT 
GCAAAAAACC GAGCTGGAGC AGGGGAGGAA TATGAAAAGG AAATTTCCAC AGCTGAAGAT 
TTTCCTAGTG GGTTTCCCCA AAATCTGCAA GTAGTTGGAC TTACAACCTC TACTACAGAA 
GTGTCCTGGG AACCACCAGT GTTGTCTGAG CGCAATGGAA AGATCACCAA CTACACAGTG 
GTGTATCGGG ACATAAATAG CCAGCAGGAT CTCACCAATG TCACTCGCGA CACACACATA 
ACATTGGTCA ATCTCAAGCC AGACACCACA TATGACATCA AGGTCAGGGC AAGAACAAAC 
AAAGGCGCTG GCCCTCTAAG TCCTAGCATT CAATCACGTA CAATGCCTGT TGACCAAGTG 
TTTGCTAAGA ATTTCAGAGT AAACGCCGTG ACAAAAACCT CAGTCTTGCT AAGCTGGGAG 
CTGCCTGATT CTTACAAATC AGCTGTGCCT TTCAAGATCT TTTACAATAA TCAACATGTA 
GAAGTTGATG GACACTCTAT GCGCAAACTG ATCAGTAATC TCAAGCCAGA TACAGAATAC 
TCCTTTGTGC TGATGAACAG GGGGAGCAGT GCAGGTGGAC TGCAGCACCG CGTGTCCATT 
CGCACAGCAC CAGATGTTTT AAAAAGCAAG CCTTTTGCTG CCAACAAATA CATGGAGGAG 
GGCAGGTTCA CCTTGCTGTT GCCTCAGGTT CAGACTACTT CCCCAGTAAG GTGGTATTAT 
ATTGTGGTTG TCCCACTGGA CAGTTCCAGC GGGGGTCTGA ATTCTCGCTG GCAGAGCCCT 
GAGGAAATGG AGCTAGAGCA GATACTTGAA GCAATAAACC AGGGATCCCG TCGTCATAGA 
AGACAGGCAA GCAAGAATAA GCCATACATT GCAGCCCGAG TAAACACACT TCCAGAATCC 
TTTATTTTGG GTGACAATAA AGAATATAAT GGGTTCATCA ACAAGCCACT GAATCAGAAT 
TTACGCTACA CCTGCTTTGT GCTAGCTGCA CTAGAAGATG GGGACACTAA AAAGTATGCA 
GCCAGCCCAT ACTCAGATGA TATTGTGGTG GAGGTGATCT CACAGGCAAA ACAACAGGAA 
GAGCCAGAAA TGCTTTGGGT CATGGGTCCT GTTCTGGCTG TAATTCTTAT AATAATTATA 
GTTATTGCCA TTCTACTTTT CAAAAGTAAA CAGGAAAGGA AAAGGACCAA CTCTCCTTCA 
GGCAAAGATG AACAGTCAGT AGGACTTAAA GACTGTCTCC TCGCTCACTC CTCAGATCCA 
GTTGAAATGA GGCGTCTAAA CTACCAAACT CCAGGTATGA GAGACCACCC TCCTATTCCA 
ATCTCTGACC TGGCAGACAA CATTGAAAGA CTGAAGGCTA ATGATGGACT CAAGTTCTCT 
CAGGAATATG AGTCTATTGA TCCAGGTCAG CAATTCACGT GGGAAAATTC AAATCTAGAA 
GTAAACAAAC CAAAGAATCG CTATGCAAAT GTCATTGCAT ATGACCATTC AAGGGTCATT 
CTTACCTCAG TGGATGGAGT TCCCGGAAGT GATTACATTA ATGCCAATTA CATAGATGGG 
TACAGGAAAC AGAATGCCTA TATAGCTACG CAGGGCCCAC TGCCAGAGAC TATCACTGAT 
TTCTGGAGAA TGGTGTGGGA GCAAAGAACC GCCACTATTG TCATGATGAC CCGGCTAGAG 
GAAAAATCAA GGGTGAAATG TGACCAGTAC TGGCCCGGTC GTGGCTCAGA GACCTATGGT 
ATGATCCAAG TCACATTAAT GGACACAGTA GAGCTTGCCA CCTACACAGT TAGGACTTTC 
ACACTTTATA AGAATGGCTC CAGTGAGAAG CGGGATGTCA GACAGTTCCA ATTTATGGCC 
TGGCCTGATC ACGGGGTCCC TGAATATCCT ACTCCTATTC TTGGATTCCT TCGCAGAGTG 
AAAGGCTGTA ATCCACCTGA TGCTGGTCCT ATGGTTGTTC ATTGCAGTGC TGGTGTGGGC 
AGAACAGGAT GCTTTATTGT TATTGATGCC ATGCTAGAAC GGGTGAAACT GGAGAAAACT 
GTGGATATTT ATGGTCATGT GACCTGCATG CGCTCCCAAC GAAATTATAT GGTGCAAACA 
GAGGACCAGT ACATCTTTAT CCATGAGGCT TTGCTTGAAG CAGTAATGTG CGGGATCACT 
GAAGTGCCAG CACGCAGTCT GTATGCACAT ATCCAGAAAC TGTCTCAGGT CCCTCCGGGT 
GAGAGTGTTA CCTCCATGGA ACTAGAGTTC AAGCTTTTGG CCAACTCTAA AGCCCACACC 
TCACGATTTA TCAGTGCCAA CCTCCCATGC AACAAGTTCA AAAACAGACT GGTAAACATC 
ATGCCATATG AACTGAGTCG TGTCTCCCTG CAGCCCATTC GTGGTGTTGA GGGATCAGAT 
TATATCAATG CAAGTTTCAT TGATGGCTAC AGGCAGCAGA AAGGCTACAT TGCTACTCAG 
GGTCCATTGG CCGAGACCAC TGAGGATTTC TGGAGAATGC TGTGGGAACA TAACTCCACC 
ATTGTGGTGA TGCTGACAAA GTTGCGAGAA ATGGGGAGGG AGAAGTGCCA TCAGTACTGG 
CCTGCAGAGC GCTCAGCACG GTACCAGTAT TTTGTGGTGG ACCCTATGGC TGAGTATAAC 
ATGCCTCAGT ATATCCTGCG TGAGTTCAAA GTGACAGATG CACGGGATGG CCAATCTCGT 
ACAATTAGAC AATTCCAGTT CACTGATTGG CCTGAACAAG GAGTGCCAAA AACAGGTGAA 
GGCTTTATTG ACTTCATTGG GCAGGTGCAC AAAACCAAGG AGCAGTTTGG GCAGGATGGG 
CCAATCACCG TTCACTGCAG CGCTGGCGTG GGGCGCACTG GAGTATTCAT CACTCTCAGT 
ATTGTACTGG AGCGTATGAG GTACGAGGGT GTGGTTGACA TGTTTCAAAC AGTGAAGACT 
TTAAGAACAC AGCGGCCAGC AATGGTACAA ACAGAGGACC AGTATCAACT GTGTTATAGA 
GCCGCACTGG AGTATCTGGG CAGCTTTGAC CACTATGCAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_018093544.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949033.1 Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X5, mRNA.

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CloneID	OXa44151
Clone ID Related Accession (Same CDS sequence)	XM_018093544.1
Accession Version	XM_018093544.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTCAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACATT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGAGATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCAAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTAACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTGCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA ATGGAGACCT GAGGATGTGG GAGATTACGA TGCTGTTATC 
ACTGGCCTCT CTCCTGAGAC CACTTATTCC ATCACTGTGG CCGCCTACAC TACTAAGGGA 
GATGGTGCTA GAAGCAAACC CAAGGTCGTT AGCACCACAG GAGCTGTGTT TGCTAAGAAT 
TTCAGAGTAA ACGCCGTGAC AAAAACCTCA GTCTTGCTAA GCTGGGAGCT GCCTGATTCT 
TACAAATCAG CTGTGCCTTT CAAGATCTTT TACAATAATC AACATGTAGA AGTTGATGGA 
CACTCTATGC GCAAACTGAT CAGTAATCTC AAGCCAGATA CAGAATACTC CTTTGTGCTG 
ATGAACAGGG GGAGCAGTGC AGGTGGACTG CAGCACCGCG TGTCCATTCG CACAGCACCA 
GATGTTTTAA AAAGCAAGCC TTTTGCTGCC AACAAATACA TGGAGGAGGG CAGGTTCACC 
TTGCTGTTGC CTCAGGTTCA GACTACTTCC CCAGTAAGGT GGTATTATAT TGTGGTTGTC 
CCACTGGACA GTTCCAGCGG GGGTCTGAAT TCTCGCTGGC AGAGCCCTGA GGAAATGGAG 
CTAGAGCAGA TACTTGAAGC AATAAACCAG GGATCCCGTC GTCATAGAAG ACAGGCAAGC 
AAGAATAAGC CATACATTGC AGCCCGAGTA AACACACTTC CAGAATCCTT TATTTTGGGT 
GACAATAAAG AATATAATGG GTTCATCAAC AAGCCACTGA ATCAGAATTT ACGCTACACC 
TGCTTTGTGC TAGCTGCACT AGAAGATGGG GACACTAAAA AGTATGCAGC CAGCCCATAC 
TCAGATGATA TTGTGGTGGA GGTGATCTCA CAGGCAAAAC AACAGGAAGA GCCAGAAATG 
CTTTGGGTCA TGGGTCCTGT TCTGGCTGTA ATTCTTATAA TAATTATAGT TATTGCCATT 
CTACTTTTCA AAAGTAAACA GGAAAGGAAA AGGACCAACT CTCCTTCAGG CAAAGATGAA 
CAGTCAGTAG GACTTAAAGA CTGTCTCCTC GCTCACTCCT CAGATCCAGT TGAAATGAGG 
CGTCTAAACT ACCAAACTCC AGGTATGAGA GACCACCCTC CTATTCCAAT CTCTGACCTG 
GCAGACAACA TTGAAAGACT GAAGGCTAAT GATGGACTCA AGTTCTCTCA GGAATATGAG 
TCTATTGATC CAGGTCAGCA ATTCACGTGG GAAAATTCAA ATCTAGAAGT AAACAAACCA 
AAGAATCGCT ATGCAAATGT CATTGCATAT GACCATTCAA GGGTCATTCT TACCTCAGTG 
GATGGAGTTC CCGGAAGTGA TTACATTAAT GCCAATTACA TAGATGGGTA CAGGAAACAG 
AATGCCTATA TAGCTACGCA GGGCCCACTG CCAGAGACTA TCACTGATTT CTGGAGAATG 
GTGTGGGAGC AAAGAACCGC CACTATTGTC ATGATGACCC GGCTAGAGGA AAAATCAAGG 
GTGAAATGTG ACCAGTACTG GCCCGGTCGT GGCTCAGAGA CCTATGGTAT GATCCAAGTC 
ACATTAATGG ACACAGTAGA GCTTGCCACC TACACAGTTA GGACTTTCAC ACTTTATAAG 
AATGGCTCCA GTGAGAAGCG GGATGTCAGA CAGTTCCAAT TTATGGCCTG GCCTGATCAC 
GGGGTCCCTG AATATCCTAC TCCTATTCTT GGATTCCTTC GCAGAGTGAA AGGCTGTAAT 
CCACCTGATG CTGGTCCTAT GGTTGTTCAT TGCAGTGCTG GTGTGGGCAG AACAGGATGC 
TTTATTGTTA TTGATGCCAT GCTAGAACGG GTGAAACTGG AGAAAACTGT GGATATTTAT 
GGTCATGTGA CCTGCATGCG CTCCCAACGA AATTATATGG TGCAAACAGA GGACCAGTAC 
ATCTTTATCC ATGAGGCTTT GCTTGAAGCA GTAATGTGCG GGATCACTGA AGTGCCAGCA 
CGCAGTCTGT ATGCACATAT CCAGAAACTG TCTCAGGTCC CTCCGGGTGA GAGTGTTACC 
TCCATGGAAC TAGAGTTCAA GCTTTTGGCC AACTCTAAAG CCCACACCTC ACGATTTATC 
AGTGCCAACC TCCCATGCAA CAAGTTCAAA AACAGACTGG TAAACATCAT GCCATATGAA 
CTGAGTCGTG TCTCCCTGCA GCCCATTCGT GGTGTTGAGG GATCAGATTA TATCAATGCA 
AGTTTCATTG ATGGCTACAG GCAGCAGAAA GGCTACATTG CTACTCAGGG TCCATTGGCC 
GAGACCACTG AGGATTTCTG GAGAATGCTG TGGGAACATA ACTCCACCAT TGTGGTGATG 
CTGACAAAGT TGCGAGAAAT GGGGAGGGAG AAGTGCCATC AGTACTGGCC TGCAGAGCGC 
TCAGCACGGT ACCAGTATTT TGTGGTGGAC CCTATGGCTG AGTATAACAT GCCTCAGTAT 
ATCCTGCGTG AGTTCAAAGT GACAGATGCA CGGGATGGCC AATCTCGTAC AATTAGACAA 
TTCCAGTTCA CTGATTGGCC TGAACAAGGA GTGCCAAAAA CAGGTGAAGG CTTTATTGAC 
TTCATTGGGC AGGTGCACAA AACCAAGGAG CAGTTTGGGC AGGATGGGCC AATCACCGTT 
CACTGCAGCG CTGGCGTGGG GCGCACTGGA GTATTCATCA CTCTCAGTAT TGTACTGGAG 
CGTATGAGGT ACGAGGGTGT GGTTGACATG TTTCAAACAG TGAAGACTTT AAGAACACAG 
CGGCCAGCAA TGGTACAAAC AGAGGACCAG TATCAACTGT GTTATAGAGC CGCACTGGAG 
TATCTGGGCA GCTTTGACCA CTATGCAACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949033.1
CDS	635..5767
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSEIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQWRPEDVGDYDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKSKQERKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_018093544.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093544.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTCAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACATT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGAGATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCAAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTAACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTGCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA ATGGAGACCT GAGGATGTGG GAGATTACGA TGCTGTTATC 
ACTGGCCTCT CTCCTGAGAC CACTTATTCC ATCACTGTGG CCGCCTACAC TACTAAGGGA 
GATGGTGCTA GAAGCAAACC CAAGGTCGTT AGCACCACAG GAGCTGTGTT TGCTAAGAAT 
TTCAGAGTAA ACGCCGTGAC AAAAACCTCA GTCTTGCTAA GCTGGGAGCT GCCTGATTCT 
TACAAATCAG CTGTGCCTTT CAAGATCTTT TACAATAATC AACATGTAGA AGTTGATGGA 
CACTCTATGC GCAAACTGAT CAGTAATCTC AAGCCAGATA CAGAATACTC CTTTGTGCTG 
ATGAACAGGG GGAGCAGTGC AGGTGGACTG CAGCACCGCG TGTCCATTCG CACAGCACCA 
GATGTTTTAA AAAGCAAGCC TTTTGCTGCC AACAAATACA TGGAGGAGGG CAGGTTCACC 
TTGCTGTTGC CTCAGGTTCA GACTACTTCC CCAGTAAGGT GGTATTATAT TGTGGTTGTC 
CCACTGGACA GTTCCAGCGG GGGTCTGAAT TCTCGCTGGC AGAGCCCTGA GGAAATGGAG 
CTAGAGCAGA TACTTGAAGC AATAAACCAG GGATCCCGTC GTCATAGAAG ACAGGCAAGC 
AAGAATAAGC CATACATTGC AGCCCGAGTA AACACACTTC CAGAATCCTT TATTTTGGGT 
GACAATAAAG AATATAATGG GTTCATCAAC AAGCCACTGA ATCAGAATTT ACGCTACACC 
TGCTTTGTGC TAGCTGCACT AGAAGATGGG GACACTAAAA AGTATGCAGC CAGCCCATAC 
TCAGATGATA TTGTGGTGGA GGTGATCTCA CAGGCAAAAC AACAGGAAGA GCCAGAAATG 
CTTTGGGTCA TGGGTCCTGT TCTGGCTGTA ATTCTTATAA TAATTATAGT TATTGCCATT 
CTACTTTTCA AAAGTAAACA GGAAAGGAAA AGGACCAACT CTCCTTCAGG CAAAGATGAA 
CAGTCAGTAG GACTTAAAGA CTGTCTCCTC GCTCACTCCT CAGATCCAGT TGAAATGAGG 
CGTCTAAACT ACCAAACTCC AGGTATGAGA GACCACCCTC CTATTCCAAT CTCTGACCTG 
GCAGACAACA TTGAAAGACT GAAGGCTAAT GATGGACTCA AGTTCTCTCA GGAATATGAG 
TCTATTGATC CAGGTCAGCA ATTCACGTGG GAAAATTCAA ATCTAGAAGT AAACAAACCA 
AAGAATCGCT ATGCAAATGT CATTGCATAT GACCATTCAA GGGTCATTCT TACCTCAGTG 
GATGGAGTTC CCGGAAGTGA TTACATTAAT GCCAATTACA TAGATGGGTA CAGGAAACAG 
AATGCCTATA TAGCTACGCA GGGCCCACTG CCAGAGACTA TCACTGATTT CTGGAGAATG 
GTGTGGGAGC AAAGAACCGC CACTATTGTC ATGATGACCC GGCTAGAGGA AAAATCAAGG 
GTGAAATGTG ACCAGTACTG GCCCGGTCGT GGCTCAGAGA CCTATGGTAT GATCCAAGTC 
ACATTAATGG ACACAGTAGA GCTTGCCACC TACACAGTTA GGACTTTCAC ACTTTATAAG 
AATGGCTCCA GTGAGAAGCG GGATGTCAGA CAGTTCCAAT TTATGGCCTG GCCTGATCAC 
GGGGTCCCTG AATATCCTAC TCCTATTCTT GGATTCCTTC GCAGAGTGAA AGGCTGTAAT 
CCACCTGATG CTGGTCCTAT GGTTGTTCAT TGCAGTGCTG GTGTGGGCAG AACAGGATGC 
TTTATTGTTA TTGATGCCAT GCTAGAACGG GTGAAACTGG AGAAAACTGT GGATATTTAT 
GGTCATGTGA CCTGCATGCG CTCCCAACGA AATTATATGG TGCAAACAGA GGACCAGTAC 
ATCTTTATCC ATGAGGCTTT GCTTGAAGCA GTAATGTGCG GGATCACTGA AGTGCCAGCA 
CGCAGTCTGT ATGCACATAT CCAGAAACTG TCTCAGGTCC CTCCGGGTGA GAGTGTTACC 
TCCATGGAAC TAGAGTTCAA GCTTTTGGCC AACTCTAAAG CCCACACCTC ACGATTTATC 
AGTGCCAACC TCCCATGCAA CAAGTTCAAA AACAGACTGG TAAACATCAT GCCATATGAA 
CTGAGTCGTG TCTCCCTGCA GCCCATTCGT GGTGTTGAGG GATCAGATTA TATCAATGCA 
AGTTTCATTG ATGGCTACAG GCAGCAGAAA GGCTACATTG CTACTCAGGG TCCATTGGCC 
GAGACCACTG AGGATTTCTG GAGAATGCTG TGGGAACATA ACTCCACCAT TGTGGTGATG 
CTGACAAAGT TGCGAGAAAT GGGGAGGGAG AAGTGCCATC AGTACTGGCC TGCAGAGCGC 
TCAGCACGGT ACCAGTATTT TGTGGTGGAC CCTATGGCTG AGTATAACAT GCCTCAGTAT 
ATCCTGCGTG AGTTCAAAGT GACAGATGCA CGGGATGGCC AATCTCGTAC AATTAGACAA 
TTCCAGTTCA CTGATTGGCC TGAACAAGGA GTGCCAAAAA CAGGTGAAGG CTTTATTGAC 
TTCATTGGGC AGGTGCACAA AACCAAGGAG CAGTTTGGGC AGGATGGGCC AATCACCGTT 
CACTGCAGCG CTGGCGTGGG GCGCACTGGA GTATTCATCA CTCTCAGTAT TGTACTGGAG 
CGTATGAGGT ACGAGGGTGT GGTTGACATG TTTCAAACAG TGAAGACTTT AAGAACACAG 
CGGCCAGCAA TGGTACAAAC AGAGGACCAG TATCAACTGT GTTATAGAGC CGCACTGGAG 
TATCTGGGCA GCTTTGACCA CTATGCAACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_018093545.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949034.1 Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X6, mRNA.

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CloneID	OXa44152
Clone ID Related Accession (Same CDS sequence)	XM_018093545.1
Accession Version	XM_018093545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTCAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACATT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGAGATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCAAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTAACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTGCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA ATGGAGACCT GAGGATGTGG GAGATTACGA TGCTGTTATC 
ACTGGCCTCT CTCCTGAGAC CACTTATTCC ATCACTGTGG CCGCCTACAC TACTAAGGGA 
GATGGTGCTA GAAGCAAACC CAAGGTCGTT AGCACCACAG GAGCTGTGTT TGCTAAGAAT 
TTCAGAGTAA ACGCCGTGAC AAAAACCTCA GTCTTGCTAA GCTGGGAGCT GCCTGATTCT 
TACAAATCAG CTGTGCCTTT CAAGATCTTT TACAATAATC AACATGTAGA AGTTGATGGA 
CACTCTATGC GCAAACTGAT CAGTAATCTC AAGCCAGATA CAGAATACTC CTTTGTGCTG 
ATGAACAGGG GGAGCAGTGC AGGTGGACTG CAGCACCGCG TGTCCATTCG CACAGCACCA 
GATGTTTTAA AAAGCAAGCC TTTTGCTGCC AACAAATACA TGGAGGAGGG CAGGTTCACC 
TTGCTGTTGC CTCAGGTTCA GACTACTTCC CCAGTAAGGT GGTATTATAT TGTGGTTGTC 
CCACTGGACA GTTCCAGCGG GGGTCTGAAT TCTCGCTGGC AGAGCCCTGA GGAAATGGAG 
CTAGAGCAGA TACTTGAAGC AATAAACCAG GGATCCCGTC GTCATAGAAG ACAGGCAAGC 
AAGAATAAGC CATACATTGC AGCCCGAGTA AACACACTTC CAGAATCCTT TATTTTGGGT 
GACAATAAAG AATATAATGG GTTCATCAAC AAGCCACTGA ATCAGAATTT ACGCTACACC 
TGCTTTGTGC TAGCTGCACT AGAAGATGGG GACACTAAAA AGTATGCAGC CAGCCCATAC 
TCAGATGATA TTGTGGTGGA GGTGATCTCA CAGGCAAAAC AACAGGAAGA GCCAGAAATG 
CTTTGGGTCA TGGGTCCTGT TCTGGCTGTA ATTCTTATAA TAATTATAGT TATTGCCATT 
CTACTTTTCA AAAGGAAAAG GACCAACTCT CCTTCAGGCA AAGATGAACA GTCAGTAGGA 
CTTAAAGACT GTCTCCTCGC TCACTCCTCA GATCCAGTTG AAATGAGGCG TCTAAACTAC 
CAAACTCCAG GTATGAGAGA CCACCCTCCT ATTCCAATCT CTGACCTGGC AGACAACATT 
GAAAGACTGA AGGCTAATGA TGGACTCAAG TTCTCTCAGG AATATGAGTC TATTGATCCA 
GGTCAGCAAT TCACGTGGGA AAATTCAAAT CTAGAAGTAA ACAAACCAAA GAATCGCTAT 
GCAAATGTCA TTGCATATGA CCATTCAAGG GTCATTCTTA CCTCAGTGGA TGGAGTTCCC 
GGAAGTGATT ACATTAATGC CAATTACATA GATGGGTACA GGAAACAGAA TGCCTATATA 
GCTACGCAGG GCCCACTGCC AGAGACTATC ACTGATTTCT GGAGAATGGT GTGGGAGCAA 
AGAACCGCCA CTATTGTCAT GATGACCCGG CTAGAGGAAA AATCAAGGGT GAAATGTGAC 
CAGTACTGGC CCGGTCGTGG CTCAGAGACC TATGGTATGA TCCAAGTCAC ATTAATGGAC 
ACAGTAGAGC TTGCCACCTA CACAGTTAGG ACTTTCACAC TTTATAAGAA TGGCTCCAGT 
GAGAAGCGGG ATGTCAGACA GTTCCAATTT ATGGCCTGGC CTGATCACGG GGTCCCTGAA 
TATCCTACTC CTATTCTTGG ATTCCTTCGC AGAGTGAAAG GCTGTAATCC ACCTGATGCT 
GGTCCTATGG TTGTTCATTG CAGTGCTGGT GTGGGCAGAA CAGGATGCTT TATTGTTATT 
GATGCCATGC TAGAACGGGT GAAACTGGAG AAAACTGTGG ATATTTATGG TCATGTGACC 
TGCATGCGCT CCCAACGAAA TTATATGGTG CAAACAGAGG ACCAGTACAT CTTTATCCAT 
GAGGCTTTGC TTGAAGCAGT AATGTGCGGG ATCACTGAAG TGCCAGCACG CAGTCTGTAT 
GCACATATCC AGAAACTGTC TCAGGTCCCT CCGGGTGAGA GTGTTACCTC CATGGAACTA 
GAGTTCAAGC TTTTGGCCAA CTCTAAAGCC CACACCTCAC GATTTATCAG TGCCAACCTC 
CCATGCAACA AGTTCAAAAA CAGACTGGTA AACATCATGC CATATGAACT GAGTCGTGTC 
TCCCTGCAGC CCATTCGTGG TGTTGAGGGA TCAGATTATA TCAATGCAAG TTTCATTGAT 
GGCTACAGGC AGCAGAAAGG CTACATTGCT ACTCAGGGTC CATTGGCCGA GACCACTGAG 
GATTTCTGGA GAATGCTGTG GGAACATAAC TCCACCATTG TGGTGATGCT GACAAAGTTG 
CGAGAAATGG GGAGGGAGAA GTGCCATCAG TACTGGCCTG CAGAGCGCTC AGCACGGTAC 
CAGTATTTTG TGGTGGACCC TATGGCTGAG TATAACATGC CTCAGTATAT CCTGCGTGAG 
TTCAAAGTGA CAGATGCACG GGATGGCCAA TCTCGTACAA TTAGACAATT CCAGTTCACT 
GATTGGCCTG AACAAGGAGT GCCAAAAACA GGTGAAGGCT TTATTGACTT CATTGGGCAG 
GTGCACAAAA CCAAGGAGCA GTTTGGGCAG GATGGGCCAA TCACCGTTCA CTGCAGCGCT 
GGCGTGGGGC GCACTGGAGT ATTCATCACT CTCAGTATTG TACTGGAGCG TATGAGGTAC 
GAGGGTGTGG TTGACATGTT TCAAACAGTG AAGACTTTAA GAACACAGCG GCCAGCAATG 
GTACAAACAG AGGACCAGTA TCAACTGTGT TATAGAGCCG CACTGGAGTA TCTGGGCAGC 
TTTGACCACT ATGCAACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949034.1
CDS	635..5755
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSEIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQWRPEDVGDYDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKRKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_018093545.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093545.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTCAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACATT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGAGATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCAAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTAACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTGCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA ATGGAGACCT GAGGATGTGG GAGATTACGA TGCTGTTATC 
ACTGGCCTCT CTCCTGAGAC CACTTATTCC ATCACTGTGG CCGCCTACAC TACTAAGGGA 
GATGGTGCTA GAAGCAAACC CAAGGTCGTT AGCACCACAG GAGCTGTGTT TGCTAAGAAT 
TTCAGAGTAA ACGCCGTGAC AAAAACCTCA GTCTTGCTAA GCTGGGAGCT GCCTGATTCT 
TACAAATCAG CTGTGCCTTT CAAGATCTTT TACAATAATC AACATGTAGA AGTTGATGGA 
CACTCTATGC GCAAACTGAT CAGTAATCTC AAGCCAGATA CAGAATACTC CTTTGTGCTG 
ATGAACAGGG GGAGCAGTGC AGGTGGACTG CAGCACCGCG TGTCCATTCG CACAGCACCA 
GATGTTTTAA AAAGCAAGCC TTTTGCTGCC AACAAATACA TGGAGGAGGG CAGGTTCACC 
TTGCTGTTGC CTCAGGTTCA GACTACTTCC CCAGTAAGGT GGTATTATAT TGTGGTTGTC 
CCACTGGACA GTTCCAGCGG GGGTCTGAAT TCTCGCTGGC AGAGCCCTGA GGAAATGGAG 
CTAGAGCAGA TACTTGAAGC AATAAACCAG GGATCCCGTC GTCATAGAAG ACAGGCAAGC 
AAGAATAAGC CATACATTGC AGCCCGAGTA AACACACTTC CAGAATCCTT TATTTTGGGT 
GACAATAAAG AATATAATGG GTTCATCAAC AAGCCACTGA ATCAGAATTT ACGCTACACC 
TGCTTTGTGC TAGCTGCACT AGAAGATGGG GACACTAAAA AGTATGCAGC CAGCCCATAC 
TCAGATGATA TTGTGGTGGA GGTGATCTCA CAGGCAAAAC AACAGGAAGA GCCAGAAATG 
CTTTGGGTCA TGGGTCCTGT TCTGGCTGTA ATTCTTATAA TAATTATAGT TATTGCCATT 
CTACTTTTCA AAAGGAAAAG GACCAACTCT CCTTCAGGCA AAGATGAACA GTCAGTAGGA 
CTTAAAGACT GTCTCCTCGC TCACTCCTCA GATCCAGTTG AAATGAGGCG TCTAAACTAC 
CAAACTCCAG GTATGAGAGA CCACCCTCCT ATTCCAATCT CTGACCTGGC AGACAACATT 
GAAAGACTGA AGGCTAATGA TGGACTCAAG TTCTCTCAGG AATATGAGTC TATTGATCCA 
GGTCAGCAAT TCACGTGGGA AAATTCAAAT CTAGAAGTAA ACAAACCAAA GAATCGCTAT 
GCAAATGTCA TTGCATATGA CCATTCAAGG GTCATTCTTA CCTCAGTGGA TGGAGTTCCC 
GGAAGTGATT ACATTAATGC CAATTACATA GATGGGTACA GGAAACAGAA TGCCTATATA 
GCTACGCAGG GCCCACTGCC AGAGACTATC ACTGATTTCT GGAGAATGGT GTGGGAGCAA 
AGAACCGCCA CTATTGTCAT GATGACCCGG CTAGAGGAAA AATCAAGGGT GAAATGTGAC 
CAGTACTGGC CCGGTCGTGG CTCAGAGACC TATGGTATGA TCCAAGTCAC ATTAATGGAC 
ACAGTAGAGC TTGCCACCTA CACAGTTAGG ACTTTCACAC TTTATAAGAA TGGCTCCAGT 
GAGAAGCGGG ATGTCAGACA GTTCCAATTT ATGGCCTGGC CTGATCACGG GGTCCCTGAA 
TATCCTACTC CTATTCTTGG ATTCCTTCGC AGAGTGAAAG GCTGTAATCC ACCTGATGCT 
GGTCCTATGG TTGTTCATTG CAGTGCTGGT GTGGGCAGAA CAGGATGCTT TATTGTTATT 
GATGCCATGC TAGAACGGGT GAAACTGGAG AAAACTGTGG ATATTTATGG TCATGTGACC 
TGCATGCGCT CCCAACGAAA TTATATGGTG CAAACAGAGG ACCAGTACAT CTTTATCCAT 
GAGGCTTTGC TTGAAGCAGT AATGTGCGGG ATCACTGAAG TGCCAGCACG CAGTCTGTAT 
GCACATATCC AGAAACTGTC TCAGGTCCCT CCGGGTGAGA GTGTTACCTC CATGGAACTA 
GAGTTCAAGC TTTTGGCCAA CTCTAAAGCC CACACCTCAC GATTTATCAG TGCCAACCTC 
CCATGCAACA AGTTCAAAAA CAGACTGGTA AACATCATGC CATATGAACT GAGTCGTGTC 
TCCCTGCAGC CCATTCGTGG TGTTGAGGGA TCAGATTATA TCAATGCAAG TTTCATTGAT 
GGCTACAGGC AGCAGAAAGG CTACATTGCT ACTCAGGGTC CATTGGCCGA GACCACTGAG 
GATTTCTGGA GAATGCTGTG GGAACATAAC TCCACCATTG TGGTGATGCT GACAAAGTTG 
CGAGAAATGG GGAGGGAGAA GTGCCATCAG TACTGGCCTG CAGAGCGCTC AGCACGGTAC 
CAGTATTTTG TGGTGGACCC TATGGCTGAG TATAACATGC CTCAGTATAT CCTGCGTGAG 
TTCAAAGTGA CAGATGCACG GGATGGCCAA TCTCGTACAA TTAGACAATT CCAGTTCACT 
GATTGGCCTG AACAAGGAGT GCCAAAAACA GGTGAAGGCT TTATTGACTT CATTGGGCAG 
GTGCACAAAA CCAAGGAGCA GTTTGGGCAG GATGGGCCAA TCACCGTTCA CTGCAGCGCT 
GGCGTGGGGC GCACTGGAGT ATTCATCACT CTCAGTATTG TACTGGAGCG TATGAGGTAC 
GAGGGTGTGG TTGACATGTT TCAAACAGTG AAGACTTTAA GAACACAGCG GCCAGCAATG 
GTACAAACAG AGGACCAGTA TCAACTGTGT TATAGAGCCG CACTGGAGTA TCTGGGCAGC 
TTTGACCACT ATGCAACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_018093546.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949035.1 Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa44153
Clone ID Related Accession (Same CDS sequence)	XM_018093546.1
Accession Version	XM_018093546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTCAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACATT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGAGATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCAAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTAACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTGCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA AGATGCTGTT ATCACTGGCC TCTCTCCTGA GACCACTTAT 
TCCATCACTG TGGCCGCCTA CACTACTAAG GGAGATGGTG CTAGAAGCAA ACCCAAGGTC 
GTTAGCACCA CAGGAGCTGT GTTTGCTAAG AATTTCAGAG TAAACGCCGT GACAAAAACC 
TCAGTCTTGC TAAGCTGGGA GCTGCCTGAT TCTTACAAAT CAGCTGTGCC TTTCAAGATC 
TTTTACAATA ATCAACATGT AGAAGTTGAT GGACACTCTA TGCGCAAACT GATCAGTAAT 
CTCAAGCCAG ATACAGAATA CTCCTTTGTG CTGATGAACA GGGGGAGCAG TGCAGGTGGA 
CTGCAGCACC GCGTGTCCAT TCGCACAGCA CCAGATGTTT TAAAAAGCAA GCCTTTTGCT 
GCCAACAAAT ACATGGAGGA GGGCAGGTTC ACCTTGCTGT TGCCTCAGGT TCAGACTACT 
TCCCCAGTAA GGTGGTATTA TATTGTGGTT GTCCCACTGG ACAGTTCCAG CGGGGGTCTG 
AATTCTCGCT GGCAGAGCCC TGAGGAAATG GAGCTAGAGC AGATACTTGA AGCAATAAAC 
CAGGGATCCC GTCGTCATAG AAGACAGGCA AGCAAGAATA AGCCATACAT TGCAGCCCGA 
GTAAACACAC TTCCAGAATC CTTTATTTTG GGTGACAATA AAGAATATAA TGGGTTCATC 
AACAAGCCAC TGAATCAGAA TTTACGCTAC ACCTGCTTTG TGCTAGCTGC ACTAGAAGAT 
GGGGACACTA AAAAGTATGC AGCCAGCCCA TACTCAGATG ATATTGTGGT GGAGGTGATC 
TCACAGGCAA AACAACAGGA AGAGCCAGAA ATGCTTTGGG TCATGGGTCC TGTTCTGGCT 
GTAATTCTTA TAATAATTAT AGTTATTGCC ATTCTACTTT TCAAAAGGAA AAGGACCAAC 
TCTCCTTCAG GCAAAGATGA ACAGTCAGTA GGACTTAAAG ACTGTCTCCT CGCTCACTCC 
TCAGATCCAG TTGAAATGAG GCGTCTAAAC TACCAAACTC CAGGTATGAG AGACCACCCT 
CCTATTCCAA TCTCTGACCT GGCAGACAAC ATTGAAAGAC TGAAGGCTAA TGATGGACTC 
AAGTTCTCTC AGGAATATGA GTCTATTGAT CCAGGTCAGC AATTCACGTG GGAAAATTCA 
AATCTAGAAG TAAACAAACC AAAGAATCGC TATGCAAATG TCATTGCATA TGACCATTCA 
AGGGTCATTC TTACCTCAGT GGATGGAGTT CCCGGAAGTG ATTACATTAA TGCCAATTAC 
ATAGATGGGT ACAGGAAACA GAATGCCTAT ATAGCTACGC AGGGCCCACT GCCAGAGACT 
ATCACTGATT TCTGGAGAAT GGTGTGGGAG CAAAGAACCG CCACTATTGT CATGATGACC 
CGGCTAGAGG AAAAATCAAG GGTGAAATGT GACCAGTACT GGCCCGGTCG TGGCTCAGAG 
ACCTATGGTA TGATCCAAGT CACATTAATG GACACAGTAG AGCTTGCCAC CTACACAGTT 
AGGACTTTCA CACTTTATAA GAATGGCTCC AGTGAGAAGC GGGATGTCAG ACAGTTCCAA 
TTTATGGCCT GGCCTGATCA CGGGGTCCCT GAATATCCTA CTCCTATTCT TGGATTCCTT 
CGCAGAGTGA AAGGCTGTAA TCCACCTGAT GCTGGTCCTA TGGTTGTTCA TTGCAGTGCT 
GGTGTGGGCA GAACAGGATG CTTTATTGTT ATTGATGCCA TGCTAGAACG GGTGAAACTG 
GAGAAAACTG TGGATATTTA TGGTCATGTG ACCTGCATGC GCTCCCAACG AAATTATATG 
GTGCAAACAG AGGACCAGTA CATCTTTATC CATGAGGCTT TGCTTGAAGC AGTAATGTGC 
GGGATCACTG AAGTGCCAGC ACGCAGTCTG TATGCACATA TCCAGAAACT GTCTCAGGTC 
CCTCCGGGTG AGAGTGTTAC CTCCATGGAA CTAGAGTTCA AGCTTTTGGC CAACTCTAAA 
GCCCACACCT CACGATTTAT CAGTGCCAAC CTCCCATGCA ACAAGTTCAA AAACAGACTG 
GTAAACATCA TGCCATATGA ACTGAGTCGT GTCTCCCTGC AGCCCATTCG TGGTGTTGAG 
GGATCAGATT ATATCAATGC AAGTTTCATT GATGGCTACA GGCAGCAGAA AGGCTACATT 
GCTACTCAGG GTCCATTGGC CGAGACCACT GAGGATTTCT GGAGAATGCT GTGGGAACAT 
AACTCCACCA TTGTGGTGAT GCTGACAAAG TTGCGAGAAA TGGGGAGGGA GAAGTGCCAT 
CAGTACTGGC CTGCAGAGCG CTCAGCACGG TACCAGTATT TTGTGGTGGA CCCTATGGCT 
GAGTATAACA TGCCTCAGTA TATCCTGCGT GAGTTCAAAG TGACAGATGC ACGGGATGGC 
CAATCTCGTA CAATTAGACA ATTCCAGTTC ACTGATTGGC CTGAACAAGG AGTGCCAAAA 
ACAGGTGAAG GCTTTATTGA CTTCATTGGG CAGGTGCACA AAACCAAGGA GCAGTTTGGG 
CAGGATGGGC CAATCACCGT TCACTGCAGC GCTGGCGTGG GGCGCACTGG AGTATTCATC 
ACTCTCAGTA TTGTACTGGA GCGTATGAGG TACGAGGGTG TGGTTGACAT GTTTCAAACA 
GTGAAGACTT TAAGAACACA GCGGCCAGCA ATGGTACAAA CAGAGGACCA GTATCAACTG 
TGTTATAGAG CCGCACTGGA GTATCTGGGC AGCTTTGACC ACTATGCAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949035.1
CDS	635..5728
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSEIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKRKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_018093546.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093546.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTCAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACATT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGAGATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCAAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTAACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTGCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA AGATGCTGTT ATCACTGGCC TCTCTCCTGA GACCACTTAT 
TCCATCACTG TGGCCGCCTA CACTACTAAG GGAGATGGTG CTAGAAGCAA ACCCAAGGTC 
GTTAGCACCA CAGGAGCTGT GTTTGCTAAG AATTTCAGAG TAAACGCCGT GACAAAAACC 
TCAGTCTTGC TAAGCTGGGA GCTGCCTGAT TCTTACAAAT CAGCTGTGCC TTTCAAGATC 
TTTTACAATA ATCAACATGT AGAAGTTGAT GGACACTCTA TGCGCAAACT GATCAGTAAT 
CTCAAGCCAG ATACAGAATA CTCCTTTGTG CTGATGAACA GGGGGAGCAG TGCAGGTGGA 
CTGCAGCACC GCGTGTCCAT TCGCACAGCA CCAGATGTTT TAAAAAGCAA GCCTTTTGCT 
GCCAACAAAT ACATGGAGGA GGGCAGGTTC ACCTTGCTGT TGCCTCAGGT TCAGACTACT 
TCCCCAGTAA GGTGGTATTA TATTGTGGTT GTCCCACTGG ACAGTTCCAG CGGGGGTCTG 
AATTCTCGCT GGCAGAGCCC TGAGGAAATG GAGCTAGAGC AGATACTTGA AGCAATAAAC 
CAGGGATCCC GTCGTCATAG AAGACAGGCA AGCAAGAATA AGCCATACAT TGCAGCCCGA 
GTAAACACAC TTCCAGAATC CTTTATTTTG GGTGACAATA AAGAATATAA TGGGTTCATC 
AACAAGCCAC TGAATCAGAA TTTACGCTAC ACCTGCTTTG TGCTAGCTGC ACTAGAAGAT 
GGGGACACTA AAAAGTATGC AGCCAGCCCA TACTCAGATG ATATTGTGGT GGAGGTGATC 
TCACAGGCAA AACAACAGGA AGAGCCAGAA ATGCTTTGGG TCATGGGTCC TGTTCTGGCT 
GTAATTCTTA TAATAATTAT AGTTATTGCC ATTCTACTTT TCAAAAGGAA AAGGACCAAC 
TCTCCTTCAG GCAAAGATGA ACAGTCAGTA GGACTTAAAG ACTGTCTCCT CGCTCACTCC 
TCAGATCCAG TTGAAATGAG GCGTCTAAAC TACCAAACTC CAGGTATGAG AGACCACCCT 
CCTATTCCAA TCTCTGACCT GGCAGACAAC ATTGAAAGAC TGAAGGCTAA TGATGGACTC 
AAGTTCTCTC AGGAATATGA GTCTATTGAT CCAGGTCAGC AATTCACGTG GGAAAATTCA 
AATCTAGAAG TAAACAAACC AAAGAATCGC TATGCAAATG TCATTGCATA TGACCATTCA 
AGGGTCATTC TTACCTCAGT GGATGGAGTT CCCGGAAGTG ATTACATTAA TGCCAATTAC 
ATAGATGGGT ACAGGAAACA GAATGCCTAT ATAGCTACGC AGGGCCCACT GCCAGAGACT 
ATCACTGATT TCTGGAGAAT GGTGTGGGAG CAAAGAACCG CCACTATTGT CATGATGACC 
CGGCTAGAGG AAAAATCAAG GGTGAAATGT GACCAGTACT GGCCCGGTCG TGGCTCAGAG 
ACCTATGGTA TGATCCAAGT CACATTAATG GACACAGTAG AGCTTGCCAC CTACACAGTT 
AGGACTTTCA CACTTTATAA GAATGGCTCC AGTGAGAAGC GGGATGTCAG ACAGTTCCAA 
TTTATGGCCT GGCCTGATCA CGGGGTCCCT GAATATCCTA CTCCTATTCT TGGATTCCTT 
CGCAGAGTGA AAGGCTGTAA TCCACCTGAT GCTGGTCCTA TGGTTGTTCA TTGCAGTGCT 
GGTGTGGGCA GAACAGGATG CTTTATTGTT ATTGATGCCA TGCTAGAACG GGTGAAACTG 
GAGAAAACTG TGGATATTTA TGGTCATGTG ACCTGCATGC GCTCCCAACG AAATTATATG 
GTGCAAACAG AGGACCAGTA CATCTTTATC CATGAGGCTT TGCTTGAAGC AGTAATGTGC 
GGGATCACTG AAGTGCCAGC ACGCAGTCTG TATGCACATA TCCAGAAACT GTCTCAGGTC 
CCTCCGGGTG AGAGTGTTAC CTCCATGGAA CTAGAGTTCA AGCTTTTGGC CAACTCTAAA 
GCCCACACCT CACGATTTAT CAGTGCCAAC CTCCCATGCA ACAAGTTCAA AAACAGACTG 
GTAAACATCA TGCCATATGA ACTGAGTCGT GTCTCCCTGC AGCCCATTCG TGGTGTTGAG 
GGATCAGATT ATATCAATGC AAGTTTCATT GATGGCTACA GGCAGCAGAA AGGCTACATT 
GCTACTCAGG GTCCATTGGC CGAGACCACT GAGGATTTCT GGAGAATGCT GTGGGAACAT 
AACTCCACCA TTGTGGTGAT GCTGACAAAG TTGCGAGAAA TGGGGAGGGA GAAGTGCCAT 
CAGTACTGGC CTGCAGAGCG CTCAGCACGG TACCAGTATT TTGTGGTGGA CCCTATGGCT 
GAGTATAACA TGCCTCAGTA TATCCTGCGT GAGTTCAAAG TGACAGATGC ACGGGATGGC 
CAATCTCGTA CAATTAGACA ATTCCAGTTC ACTGATTGGC CTGAACAAGG AGTGCCAAAA 
ACAGGTGAAG GCTTTATTGA CTTCATTGGG CAGGTGCACA AAACCAAGGA GCAGTTTGGG 
CAGGATGGGC CAATCACCGT TCACTGCAGC GCTGGCGTGG GGCGCACTGG AGTATTCATC 
ACTCTCAGTA TTGTACTGGA GCGTATGAGG TACGAGGGTG TGGTTGACAT GTTTCAAACA 
GTGAAGACTT TAAGAACACA GCGGCCAGCA ATGGTACAAA CAGAGGACCA GTATCAACTG 
TGTTATAGAG CCGCACTGGA GTATCTGGGC AGCTTTGACC ACTATGCAAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_031900923.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756783.1 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X2, mRNA.

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CloneID	OXa57128
Clone ID Related Accession (Same CDS sequence)	XM_031900923.1 , XM_031900922.1
Accession Version	XM_031900923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAC ACCCTCAGCC CCGCCACAGG ATGTGGAGTG CTCGAGTCTT 
AGCTCCACTG CCATCCGGGT AAGTTGGGTG GCCCCTCCAG CTTCCAGCCG CAACGGGGCC 
CTGACCCGCT ACTCACTTGC CTACCAAGCC CTGGCTGGGG AGGACACATC CCGGCATGTT 
GTGGACAGCA TCTCACCAGA TATCTCCAGC TGGGAGATCA AGGATCTGGA GAAATGGACT 
GAGTACAAAG TATGGGTGCG AGCTCATACA GATGTAGGGG CTGGGCCTGA AAGCTCCCCC 
ATCATTGTGC GGACAGATGA GGATGTGCCG AGTGCACCAC CAAGAAAGGT TGAAGTAGAC 
TCTGTAAACT CCACATCTGT TCGTGTGACA TGGAAGTCTC CTCTACCAAC CAAGCAAAAT 
GGGCAAATTA GGGGGTATCA AGTCACTTAT GTTCGTCTAG AAGGTGGAGA GCCTCGGGGG 
AATCCCATCA TAAAAGATGT TATGCTGTCA GAGGCTCAAT GGAGACCTGA GGATGTGGGA 
GATTACGATG CTGTTATCAC TGGCCTCTCT CCTGAGACCA CTTATTCCAT CACTGTGGCC 
GCCTACACTA CTAAGGGAGA TGGTGCTAGA AGCAAACCCA AGGTCGTTAG CACCACAGGA 
GCTGTGCCTG GTAAACCCAC TATGATGATC AGCACCACAG CACTGAACAC TGCTCTGATC 
CAGTGGCATC CTCCAAGAGA GATTGTGGGA GAGTTGTTAG GATATCGTTT GCAGTACAAG 
CGAGTGGATG AAGACAGGTT TATTACAATT GATTTTAGCA AGAATGAACA TCACCACACA 
GTGACTGGGC TACATAAAGG TGCAAGCTAT ATATTCAAGC TTTCTGCAAA AAACCGAGCT 
GGAGCAGGGG AGGAATATGA AAAGGAAATT TCCACAGCTG AAGATTTTCC TAGTGGGTTT 
CCCCAAAATC TGCAAGTAGT TGGACTTACA ACCTCTACTA CAGAAGTGTC CTGGGAACCA 
CCAGTGTTGT CTGAGCGCAA TGGAAAGATC ACCAACTACA CAGTGGTGTA TCGGGACATA 
AATAGCCAGC AGGATCTCAC CAATGTCACT CGCGACACAC ACATAACATT GGTCAATCTC 
AAGCCAGACA CCACATATGA CATCAAGGTC AGGGCAAGAA CAAACAAAGG CGCTGGCCCT 
CTAAGTCCTA GCATTCAATC ACGTACAATG CCTGTTGACC AAGTGTTTGC TAAGAATTTC 
AGAGTAAACG CCGTGACAAA AACCTCAGTC TTGCTAAGCT GGGAGCTGCC TGATTCTTAC 
AAATCAGCTG TGCCTTTCAA GATCTTTTAC AATAATCAAC ATGTAGAAGT TGATGGACAC 
TCTATGCGCA AACTGATCAG TAATCTCAAG CCAGATACAG AATACTCCTT TGTGCTGATG 
AACAGGGGGA GCAGTGCAGG TGGACTGCAG CACCGCGTGT CCATTCGCAC AGCACCAGAT 
GTTTTAAAAA GCAAGCCTTT TGCTGCCAAC AAATACATGG AGGAGGGCAG GTTCACCTTG 
CTGTTGCCTC AGGTTCAGAC TACTTCCCCA GTAAGGTGGT ATTATATTGT GGTTGTCCCA 
CTGGACAGTT CCAGCGGGGG TCTGAATTCT CGCTGGCAGA GCCCTGAGGA AATGGAGCTA 
GAGCAGATAC TTGAAGCAAT AAACCAGGGA TCCCGTCGTC ATAGAAGACA GGCAAGCAAG 
AATAAGCCAT ACATTGCAGC CCGAGTAAAC ACACTTCCAG AATCCTTTAT TTTGGGTGAC 
AATAAAGAAT ATAATGGGTT CATCAACAAG CCACTGAATC AGAATTTACG CTACACCTGC 
TTTGTGCTAG CTGCACTAGA AGATGGGGAC ACTAAAAAGT ATGCAGCCAG CCCATACTCA 
GATGATATTG TGGTGGAGGT GATCTCACAG GCAAAACAAC AGGAAGAGCC AGAAATGCTT 
TGGGTCATGG GTCCTGTTCT GGCTGTAATT CTTATAATAA TTATAGTTAT TGCCATTCTA 
CTTTTCAAAA GTAAACAGGA AAGGAAAAGG ACCAACTCTC CTTCAGGCAA AGATGAACAG 
TCAGTAGGAC TTAAAGACTG TCTCCTCGCT CACTCCTCAG ATCCAGTTGA AATGAGGCGT 
CTAAACTACC AAACTCCAGG TTCCAGTGTC CCCAGTTACC CGAATATCTC AAGTATGAGA 
GACCACCCTC CTATTCCAAT CTCTGACCTG GCAGACAACA TTGAAAGACT GAAGGCTAAT 
GATGGACTCA AGTTCTCTCA GGAATATGAG TCTATTGATC CAGGTCAGCA ATTCACGTGG 
GAAAATTCAA ATCTAGAAGT AAACAAACCA AAGAATCGCT ATGCAAATGT CATTGCATAT 
GACCATTCAA GGGTCATTCT TACCTCAGTG GATGGAGTTC CCGGAAGTGA TTACATTAAT 
GCCAATTACA TAGATGGGTA CAGGAAACAG AATGCCTATA TAGCTACGCA GGGCCCACTG 
CCAGAGACTA TCACTGATTT CTGGAGAATG GTGTGGGAGC AAAGAACCGC CACTATTGTC 
ATGATGACCC GGCTAGAGGA AAAATCAAGG GTGAAATGTG ACCAGTACTG GCCCGGTCGT 
GGCTCAGAGA CCTATGGTAT GATCCAAGTC ACATTAATGG ACACAGTAGA GCTTGCCACC 
TACACAGTTA GGACTTTCAC ACTTTATAAG AATGGCTCCA GTGAGAAGCG GGATGTCAGA 
CAGTTCCAAT TTATGGCCTG GCCTGATCAC GGGGTCCCTG AATATCCTAC TCCTATTCTT 
GGATTCCTTC GCAGAGTGAA AGGCTGTAAT CCACCTGATG CTGGTCCTAT GGTTGTTCAT 
TGCAGTGCTG GCGTGGGCAG AACAGGATGC TTTATTGTTA TTGATGCCAT GCTAGAACGG 
GTGAAACTGG AGAAAACTGT GGATATTTAT GGTCATGTGA CCTGCATGCG CTCCCAACGA 
AATTATATGG TGCAAACAGA GGACCAGTAC ATCTTTATCC ATGAGGCTTT GCTTGAAGCA 
GTAATGTGCG GGATCACTGA AGTGCCAGCA CGCAGTCTGT ATGCACATAT CCAGAAACTG 
TCTCAGGTCC CTCCGGGTGA GAGTGTTACC TCCATGGAAC TAGAGTTCAA GCTTTTGGCC 
AACTCTAAAG CCCACACCTC ACGATTTATC AGTGCCAACC TCCCATGCAA CAAGTTCAAA 
AACAGACTGG TAAACATCAT GCCATATGAA CTGAGTCGTG TCTCCCTGCA GCCCATTCGT 
GGTGTTGAGG GATCAGATTA TATCAATGCA AGTTTCATTG ATGGCTACAG GCAGCAGAAA 
GGCTACATTG CTACTCAGGG TCCATTGGCC GAGACCACTG AGGATTTCTG GAGAATGCTG 
TGGGAACATA ACTCCACCAT TGTGGTGATG CTGACAAAGT TGCGAGAAAT GGGGAGGGAG 
AAGTGCCATC AGTACTGGCC TGCAGAGCGC TCAGCACGGT ACCAGTATTT TGTGGTGGAC 
CCTATGGCTG AGTATAACAT GCCTCAGTAT ATCCTGCGTG AGTTCAAAGT GACAGATGCA 
CGGGATGGCC AATCTCGTAC AATTAGACAA TTCCAGTTCA CTGATTGGCC TGAACAAGGA 
GTGCCAAAAA CAGGTGAAGG CTTTATTGAC TTCATTGGGC AGGTGCACAA AACCAAGGAG 
CAGTTTGGGC AGGATGGGCC AATCACCGTT CACTGCAGCG CTGGCGTGGG CCGCACTGGA 
GTATTCATCA CTCTCAGTAT TGTACTGGAG CGTATGAGGT ACGAGGGTGT GGTTGACATG 
TTTCAAACAG TGAAGACTTT AAGAACACAG CGGCCAGCAA TGGTACAAAC AGAGGACCAG 
TATCAACTGT GCTATAGAGC CGCACTGGAG TATCTGGGCA GCTTTGACCA CTATGCAACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756783.1
CDS	494..6256
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQWRPEDVGDYDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKSKQERKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGSSVPSYPNISSMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_031900923.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900923.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAC ACCCTCAGCC CCGCCACAGG ATGTGGAGTG CTCGAGTCTT 
AGCTCCACTG CCATCCGGGT AAGTTGGGTG GCCCCTCCAG CTTCCAGCCG CAACGGGGCC 
CTGACCCGCT ACTCACTTGC CTACCAAGCC CTGGCTGGGG AGGACACATC CCGGCATGTT 
GTGGACAGCA TCTCACCAGA TATCTCCAGC TGGGAGATCA AGGATCTGGA GAAATGGACT 
GAGTACAAAG TATGGGTGCG AGCTCATACA GATGTAGGGG CTGGGCCTGA AAGCTCCCCC 
ATCATTGTGC GGACAGATGA GGATGTGCCG AGTGCACCAC CAAGAAAGGT TGAAGTAGAC 
TCTGTAAACT CCACATCTGT TCGTGTGACA TGGAAGTCTC CTCTACCAAC CAAGCAAAAT 
GGGCAAATTA GGGGGTATCA AGTCACTTAT GTTCGTCTAG AAGGTGGAGA GCCTCGGGGG 
AATCCCATCA TAAAAGATGT TATGCTGTCA GAGGCTCAAT GGAGACCTGA GGATGTGGGA 
GATTACGATG CTGTTATCAC TGGCCTCTCT CCTGAGACCA CTTATTCCAT CACTGTGGCC 
GCCTACACTA CTAAGGGAGA TGGTGCTAGA AGCAAACCCA AGGTCGTTAG CACCACAGGA 
GCTGTGCCTG GTAAACCCAC TATGATGATC AGCACCACAG CACTGAACAC TGCTCTGATC 
CAGTGGCATC CTCCAAGAGA GATTGTGGGA GAGTTGTTAG GATATCGTTT GCAGTACAAG 
CGAGTGGATG AAGACAGGTT TATTACAATT GATTTTAGCA AGAATGAACA TCACCACACA 
GTGACTGGGC TACATAAAGG TGCAAGCTAT ATATTCAAGC TTTCTGCAAA AAACCGAGCT 
GGAGCAGGGG AGGAATATGA AAAGGAAATT TCCACAGCTG AAGATTTTCC TAGTGGGTTT 
CCCCAAAATC TGCAAGTAGT TGGACTTACA ACCTCTACTA CAGAAGTGTC CTGGGAACCA 
CCAGTGTTGT CTGAGCGCAA TGGAAAGATC ACCAACTACA CAGTGGTGTA TCGGGACATA 
AATAGCCAGC AGGATCTCAC CAATGTCACT CGCGACACAC ACATAACATT GGTCAATCTC 
AAGCCAGACA CCACATATGA CATCAAGGTC AGGGCAAGAA CAAACAAAGG CGCTGGCCCT 
CTAAGTCCTA GCATTCAATC ACGTACAATG CCTGTTGACC AAGTGTTTGC TAAGAATTTC 
AGAGTAAACG CCGTGACAAA AACCTCAGTC TTGCTAAGCT GGGAGCTGCC TGATTCTTAC 
AAATCAGCTG TGCCTTTCAA GATCTTTTAC AATAATCAAC ATGTAGAAGT TGATGGACAC 
TCTATGCGCA AACTGATCAG TAATCTCAAG CCAGATACAG AATACTCCTT TGTGCTGATG 
AACAGGGGGA GCAGTGCAGG TGGACTGCAG CACCGCGTGT CCATTCGCAC AGCACCAGAT 
GTTTTAAAAA GCAAGCCTTT TGCTGCCAAC AAATACATGG AGGAGGGCAG GTTCACCTTG 
CTGTTGCCTC AGGTTCAGAC TACTTCCCCA GTAAGGTGGT ATTATATTGT GGTTGTCCCA 
CTGGACAGTT CCAGCGGGGG TCTGAATTCT CGCTGGCAGA GCCCTGAGGA AATGGAGCTA 
GAGCAGATAC TTGAAGCAAT AAACCAGGGA TCCCGTCGTC ATAGAAGACA GGCAAGCAAG 
AATAAGCCAT ACATTGCAGC CCGAGTAAAC ACACTTCCAG AATCCTTTAT TTTGGGTGAC 
AATAAAGAAT ATAATGGGTT CATCAACAAG CCACTGAATC AGAATTTACG CTACACCTGC 
TTTGTGCTAG CTGCACTAGA AGATGGGGAC ACTAAAAAGT ATGCAGCCAG CCCATACTCA 
GATGATATTG TGGTGGAGGT GATCTCACAG GCAAAACAAC AGGAAGAGCC AGAAATGCTT 
TGGGTCATGG GTCCTGTTCT GGCTGTAATT CTTATAATAA TTATAGTTAT TGCCATTCTA 
CTTTTCAAAA GTAAACAGGA AAGGAAAAGG ACCAACTCTC CTTCAGGCAA AGATGAACAG 
TCAGTAGGAC TTAAAGACTG TCTCCTCGCT CACTCCTCAG ATCCAGTTGA AATGAGGCGT 
CTAAACTACC AAACTCCAGG TTCCAGTGTC CCCAGTTACC CGAATATCTC AAGTATGAGA 
GACCACCCTC CTATTCCAAT CTCTGACCTG GCAGACAACA TTGAAAGACT GAAGGCTAAT 
GATGGACTCA AGTTCTCTCA GGAATATGAG TCTATTGATC CAGGTCAGCA ATTCACGTGG 
GAAAATTCAA ATCTAGAAGT AAACAAACCA AAGAATCGCT ATGCAAATGT CATTGCATAT 
GACCATTCAA GGGTCATTCT TACCTCAGTG GATGGAGTTC CCGGAAGTGA TTACATTAAT 
GCCAATTACA TAGATGGGTA CAGGAAACAG AATGCCTATA TAGCTACGCA GGGCCCACTG 
CCAGAGACTA TCACTGATTT CTGGAGAATG GTGTGGGAGC AAAGAACCGC CACTATTGTC 
ATGATGACCC GGCTAGAGGA AAAATCAAGG GTGAAATGTG ACCAGTACTG GCCCGGTCGT 
GGCTCAGAGA CCTATGGTAT GATCCAAGTC ACATTAATGG ACACAGTAGA GCTTGCCACC 
TACACAGTTA GGACTTTCAC ACTTTATAAG AATGGCTCCA GTGAGAAGCG GGATGTCAGA 
CAGTTCCAAT TTATGGCCTG GCCTGATCAC GGGGTCCCTG AATATCCTAC TCCTATTCTT 
GGATTCCTTC GCAGAGTGAA AGGCTGTAAT CCACCTGATG CTGGTCCTAT GGTTGTTCAT 
TGCAGTGCTG GCGTGGGCAG AACAGGATGC TTTATTGTTA TTGATGCCAT GCTAGAACGG 
GTGAAACTGG AGAAAACTGT GGATATTTAT GGTCATGTGA CCTGCATGCG CTCCCAACGA 
AATTATATGG TGCAAACAGA GGACCAGTAC ATCTTTATCC ATGAGGCTTT GCTTGAAGCA 
GTAATGTGCG GGATCACTGA AGTGCCAGCA CGCAGTCTGT ATGCACATAT CCAGAAACTG 
TCTCAGGTCC CTCCGGGTGA GAGTGTTACC TCCATGGAAC TAGAGTTCAA GCTTTTGGCC 
AACTCTAAAG CCCACACCTC ACGATTTATC AGTGCCAACC TCCCATGCAA CAAGTTCAAA 
AACAGACTGG TAAACATCAT GCCATATGAA CTGAGTCGTG TCTCCCTGCA GCCCATTCGT 
GGTGTTGAGG GATCAGATTA TATCAATGCA AGTTTCATTG ATGGCTACAG GCAGCAGAAA 
GGCTACATTG CTACTCAGGG TCCATTGGCC GAGACCACTG AGGATTTCTG GAGAATGCTG 
TGGGAACATA ACTCCACCAT TGTGGTGATG CTGACAAAGT TGCGAGAAAT GGGGAGGGAG 
AAGTGCCATC AGTACTGGCC TGCAGAGCGC TCAGCACGGT ACCAGTATTT TGTGGTGGAC 
CCTATGGCTG AGTATAACAT GCCTCAGTAT ATCCTGCGTG AGTTCAAAGT GACAGATGCA 
CGGGATGGCC AATCTCGTAC AATTAGACAA TTCCAGTTCA CTGATTGGCC TGAACAAGGA 
GTGCCAAAAA CAGGTGAAGG CTTTATTGAC TTCATTGGGC AGGTGCACAA AACCAAGGAG 
CAGTTTGGGC AGGATGGGCC AATCACCGTT CACTGCAGCG CTGGCGTGGG CCGCACTGGA 
GTATTCATCA CTCTCAGTAT TGTACTGGAG CGTATGAGGT ACGAGGGTGT GGTTGACATG 
TTTCAAACAG TGAAGACTTT AAGAACACAG CGGCCAGCAA TGGTACAAAC AGAGGACCAG 
TATCAACTGT GCTATAGAGC CGCACTGGAG TATCTGGGCA GCTTTGACCA CTATGCAACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_031900922.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756782.1 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X1, mRNA.

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CloneID	OXa57128
Clone ID Related Accession (Same CDS sequence)	XM_031900923.1 , XM_031900922.1
Accession Version	XM_031900922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAC ACCCTCAGCC CCGCCACAGG ATGTGGAGTG CTCGAGTCTT 
AGCTCCACTG CCATCCGGGT AAGTTGGGTG GCCCCTCCAG CTTCCAGCCG CAACGGGGCC 
CTGACCCGCT ACTCACTTGC CTACCAAGCC CTGGCTGGGG AGGACACATC CCGGCATGTT 
GTGGACAGCA TCTCACCAGA TATCTCCAGC TGGGAGATCA AGGATCTGGA GAAATGGACT 
GAGTACAAAG TATGGGTGCG AGCTCATACA GATGTAGGGG CTGGGCCTGA AAGCTCCCCC 
ATCATTGTGC GGACAGATGA GGATGTGCCG AGTGCACCAC CAAGAAAGGT TGAAGTAGAC 
TCTGTAAACT CCACATCTGT TCGTGTGACA TGGAAGTCTC CTCTACCAAC CAAGCAAAAT 
GGGCAAATTA GGGGGTATCA AGTCACTTAT GTTCGTCTAG AAGGTGGAGA GCCTCGGGGG 
AATCCCATCA TAAAAGATGT TATGCTGTCA GAGGCTCAAT GGAGACCTGA GGATGTGGGA 
GATTACGATG CTGTTATCAC TGGCCTCTCT CCTGAGACCA CTTATTCCAT CACTGTGGCC 
GCCTACACTA CTAAGGGAGA TGGTGCTAGA AGCAAACCCA AGGTCGTTAG CACCACAGGA 
GCTGTGCCTG GTAAACCCAC TATGATGATC AGCACCACAG CACTGAACAC TGCTCTGATC 
CAGTGGCATC CTCCAAGAGA GATTGTGGGA GAGTTGTTAG GATATCGTTT GCAGTACAAG 
CGAGTGGATG AAGACAGGTT TATTACAATT GATTTTAGCA AGAATGAACA TCACCACACA 
GTGACTGGGC TACATAAAGG TGCAAGCTAT ATATTCAAGC TTTCTGCAAA AAACCGAGCT 
GGAGCAGGGG AGGAATATGA AAAGGAAATT TCCACAGCTG AAGATTTTCC TAGTGGGTTT 
CCCCAAAATC TGCAAGTAGT TGGACTTACA ACCTCTACTA CAGAAGTGTC CTGGGAACCA 
CCAGTGTTGT CTGAGCGCAA TGGAAAGATC ACCAACTACA CAGTGGTGTA TCGGGACATA 
AATAGCCAGC AGGATCTCAC CAATGTCACT CGCGACACAC ACATAACATT GGTCAATCTC 
AAGCCAGACA CCACATATGA CATCAAGGTC AGGGCAAGAA CAAACAAAGG CGCTGGCCCT 
CTAAGTCCTA GCATTCAATC ACGTACAATG CCTGTTGACC AAGTGTTTGC TAAGAATTTC 
AGAGTAAACG CCGTGACAAA AACCTCAGTC TTGCTAAGCT GGGAGCTGCC TGATTCTTAC 
AAATCAGCTG TGCCTTTCAA GATCTTTTAC AATAATCAAC ATGTAGAAGT TGATGGACAC 
TCTATGCGCA AACTGATCAG TAATCTCAAG CCAGATACAG AATACTCCTT TGTGCTGATG 
AACAGGGGGA GCAGTGCAGG TGGACTGCAG CACCGCGTGT CCATTCGCAC AGCACCAGAT 
GTTTTAAAAA GCAAGCCTTT TGCTGCCAAC AAATACATGG AGGAGGGCAG GTTCACCTTG 
CTGTTGCCTC AGGTTCAGAC TACTTCCCCA GTAAGGTGGT ATTATATTGT GGTTGTCCCA 
CTGGACAGTT CCAGCGGGGG TCTGAATTCT CGCTGGCAGA GCCCTGAGGA AATGGAGCTA 
GAGCAGATAC TTGAAGCAAT AAACCAGGGA TCCCGTCGTC ATAGAAGACA GGCAAGCAAG 
AATAAGCCAT ACATTGCAGC CCGAGTAAAC ACACTTCCAG AATCCTTTAT TTTGGGTGAC 
AATAAAGAAT ATAATGGGTT CATCAACAAG CCACTGAATC AGAATTTACG CTACACCTGC 
TTTGTGCTAG CTGCACTAGA AGATGGGGAC ACTAAAAAGT ATGCAGCCAG CCCATACTCA 
GATGATATTG TGGTGGAGGT GATCTCACAG GCAAAACAAC AGGAAGAGCC AGAAATGCTT 
TGGGTCATGG GTCCTGTTCT GGCTGTAATT CTTATAATAA TTATAGTTAT TGCCATTCTA 
CTTTTCAAAA GTAAACAGGA AAGGAAAAGG ACCAACTCTC CTTCAGGCAA AGATGAACAG 
TCAGTAGGAC TTAAAGACTG TCTCCTCGCT CACTCCTCAG ATCCAGTTGA AATGAGGCGT 
CTAAACTACC AAACTCCAGG TTCCAGTGTC CCCAGTTACC CGAATATCTC AAGTATGAGA 
GACCACCCTC CTATTCCAAT CTCTGACCTG GCAGACAACA TTGAAAGACT GAAGGCTAAT 
GATGGACTCA AGTTCTCTCA GGAATATGAG TCTATTGATC CAGGTCAGCA ATTCACGTGG 
GAAAATTCAA ATCTAGAAGT AAACAAACCA AAGAATCGCT ATGCAAATGT CATTGCATAT 
GACCATTCAA GGGTCATTCT TACCTCAGTG GATGGAGTTC CCGGAAGTGA TTACATTAAT 
GCCAATTACA TAGATGGGTA CAGGAAACAG AATGCCTATA TAGCTACGCA GGGCCCACTG 
CCAGAGACTA TCACTGATTT CTGGAGAATG GTGTGGGAGC AAAGAACCGC CACTATTGTC 
ATGATGACCC GGCTAGAGGA AAAATCAAGG GTGAAATGTG ACCAGTACTG GCCCGGTCGT 
GGCTCAGAGA CCTATGGTAT GATCCAAGTC ACATTAATGG ACACAGTAGA GCTTGCCACC 
TACACAGTTA GGACTTTCAC ACTTTATAAG AATGGCTCCA GTGAGAAGCG GGATGTCAGA 
CAGTTCCAAT TTATGGCCTG GCCTGATCAC GGGGTCCCTG AATATCCTAC TCCTATTCTT 
GGATTCCTTC GCAGAGTGAA AGGCTGTAAT CCACCTGATG CTGGTCCTAT GGTTGTTCAT 
TGCAGTGCTG GCGTGGGCAG AACAGGATGC TTTATTGTTA TTGATGCCAT GCTAGAACGG 
GTGAAACTGG AGAAAACTGT GGATATTTAT GGTCATGTGA CCTGCATGCG CTCCCAACGA 
AATTATATGG TGCAAACAGA GGACCAGTAC ATCTTTATCC ATGAGGCTTT GCTTGAAGCA 
GTAATGTGCG GGATCACTGA AGTGCCAGCA CGCAGTCTGT ATGCACATAT CCAGAAACTG 
TCTCAGGTCC CTCCGGGTGA GAGTGTTACC TCCATGGAAC TAGAGTTCAA GCTTTTGGCC 
AACTCTAAAG CCCACACCTC ACGATTTATC AGTGCCAACC TCCCATGCAA CAAGTTCAAA 
AACAGACTGG TAAACATCAT GCCATATGAA CTGAGTCGTG TCTCCCTGCA GCCCATTCGT 
GGTGTTGAGG GATCAGATTA TATCAATGCA AGTTTCATTG ATGGCTACAG GCAGCAGAAA 
GGCTACATTG CTACTCAGGG TCCATTGGCC GAGACCACTG AGGATTTCTG GAGAATGCTG 
TGGGAACATA ACTCCACCAT TGTGGTGATG CTGACAAAGT TGCGAGAAAT GGGGAGGGAG 
AAGTGCCATC AGTACTGGCC TGCAGAGCGC TCAGCACGGT ACCAGTATTT TGTGGTGGAC 
CCTATGGCTG AGTATAACAT GCCTCAGTAT ATCCTGCGTG AGTTCAAAGT GACAGATGCA 
CGGGATGGCC AATCTCGTAC AATTAGACAA TTCCAGTTCA CTGATTGGCC TGAACAAGGA 
GTGCCAAAAA CAGGTGAAGG CTTTATTGAC TTCATTGGGC AGGTGCACAA AACCAAGGAG 
CAGTTTGGGC AGGATGGGCC AATCACCGTT CACTGCAGCG CTGGCGTGGG CCGCACTGGA 
GTATTCATCA CTCTCAGTAT TGTACTGGAG CGTATGAGGT ACGAGGGTGT GGTTGACATG 
TTTCAAACAG TGAAGACTTT AAGAACACAG CGGCCAGCAA TGGTACAAAC AGAGGACCAG 
TATCAACTGT GCTATAGAGC CGCACTGGAG TATCTGGGCA GCTTTGACCA CTATGCAACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756782.1
CDS	636..6398
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQWRPEDVGDYDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKSKQERKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGSSVPSYPNISSMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_031900922.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900922.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAC ACCCTCAGCC CCGCCACAGG ATGTGGAGTG CTCGAGTCTT 
AGCTCCACTG CCATCCGGGT AAGTTGGGTG GCCCCTCCAG CTTCCAGCCG CAACGGGGCC 
CTGACCCGCT ACTCACTTGC CTACCAAGCC CTGGCTGGGG AGGACACATC CCGGCATGTT 
GTGGACAGCA TCTCACCAGA TATCTCCAGC TGGGAGATCA AGGATCTGGA GAAATGGACT 
GAGTACAAAG TATGGGTGCG AGCTCATACA GATGTAGGGG CTGGGCCTGA AAGCTCCCCC 
ATCATTGTGC GGACAGATGA GGATGTGCCG AGTGCACCAC CAAGAAAGGT TGAAGTAGAC 
TCTGTAAACT CCACATCTGT TCGTGTGACA TGGAAGTCTC CTCTACCAAC CAAGCAAAAT 
GGGCAAATTA GGGGGTATCA AGTCACTTAT GTTCGTCTAG AAGGTGGAGA GCCTCGGGGG 
AATCCCATCA TAAAAGATGT TATGCTGTCA GAGGCTCAAT GGAGACCTGA GGATGTGGGA 
GATTACGATG CTGTTATCAC TGGCCTCTCT CCTGAGACCA CTTATTCCAT CACTGTGGCC 
GCCTACACTA CTAAGGGAGA TGGTGCTAGA AGCAAACCCA AGGTCGTTAG CACCACAGGA 
GCTGTGCCTG GTAAACCCAC TATGATGATC AGCACCACAG CACTGAACAC TGCTCTGATC 
CAGTGGCATC CTCCAAGAGA GATTGTGGGA GAGTTGTTAG GATATCGTTT GCAGTACAAG 
CGAGTGGATG AAGACAGGTT TATTACAATT GATTTTAGCA AGAATGAACA TCACCACACA 
GTGACTGGGC TACATAAAGG TGCAAGCTAT ATATTCAAGC TTTCTGCAAA AAACCGAGCT 
GGAGCAGGGG AGGAATATGA AAAGGAAATT TCCACAGCTG AAGATTTTCC TAGTGGGTTT 
CCCCAAAATC TGCAAGTAGT TGGACTTACA ACCTCTACTA CAGAAGTGTC CTGGGAACCA 
CCAGTGTTGT CTGAGCGCAA TGGAAAGATC ACCAACTACA CAGTGGTGTA TCGGGACATA 
AATAGCCAGC AGGATCTCAC CAATGTCACT CGCGACACAC ACATAACATT GGTCAATCTC 
AAGCCAGACA CCACATATGA CATCAAGGTC AGGGCAAGAA CAAACAAAGG CGCTGGCCCT 
CTAAGTCCTA GCATTCAATC ACGTACAATG CCTGTTGACC AAGTGTTTGC TAAGAATTTC 
AGAGTAAACG CCGTGACAAA AACCTCAGTC TTGCTAAGCT GGGAGCTGCC TGATTCTTAC 
AAATCAGCTG TGCCTTTCAA GATCTTTTAC AATAATCAAC ATGTAGAAGT TGATGGACAC 
TCTATGCGCA AACTGATCAG TAATCTCAAG CCAGATACAG AATACTCCTT TGTGCTGATG 
AACAGGGGGA GCAGTGCAGG TGGACTGCAG CACCGCGTGT CCATTCGCAC AGCACCAGAT 
GTTTTAAAAA GCAAGCCTTT TGCTGCCAAC AAATACATGG AGGAGGGCAG GTTCACCTTG 
CTGTTGCCTC AGGTTCAGAC TACTTCCCCA GTAAGGTGGT ATTATATTGT GGTTGTCCCA 
CTGGACAGTT CCAGCGGGGG TCTGAATTCT CGCTGGCAGA GCCCTGAGGA AATGGAGCTA 
GAGCAGATAC TTGAAGCAAT AAACCAGGGA TCCCGTCGTC ATAGAAGACA GGCAAGCAAG 
AATAAGCCAT ACATTGCAGC CCGAGTAAAC ACACTTCCAG AATCCTTTAT TTTGGGTGAC 
AATAAAGAAT ATAATGGGTT CATCAACAAG CCACTGAATC AGAATTTACG CTACACCTGC 
TTTGTGCTAG CTGCACTAGA AGATGGGGAC ACTAAAAAGT ATGCAGCCAG CCCATACTCA 
GATGATATTG TGGTGGAGGT GATCTCACAG GCAAAACAAC AGGAAGAGCC AGAAATGCTT 
TGGGTCATGG GTCCTGTTCT GGCTGTAATT CTTATAATAA TTATAGTTAT TGCCATTCTA 
CTTTTCAAAA GTAAACAGGA AAGGAAAAGG ACCAACTCTC CTTCAGGCAA AGATGAACAG 
TCAGTAGGAC TTAAAGACTG TCTCCTCGCT CACTCCTCAG ATCCAGTTGA AATGAGGCGT 
CTAAACTACC AAACTCCAGG TTCCAGTGTC CCCAGTTACC CGAATATCTC AAGTATGAGA 
GACCACCCTC CTATTCCAAT CTCTGACCTG GCAGACAACA TTGAAAGACT GAAGGCTAAT 
GATGGACTCA AGTTCTCTCA GGAATATGAG TCTATTGATC CAGGTCAGCA ATTCACGTGG 
GAAAATTCAA ATCTAGAAGT AAACAAACCA AAGAATCGCT ATGCAAATGT CATTGCATAT 
GACCATTCAA GGGTCATTCT TACCTCAGTG GATGGAGTTC CCGGAAGTGA TTACATTAAT 
GCCAATTACA TAGATGGGTA CAGGAAACAG AATGCCTATA TAGCTACGCA GGGCCCACTG 
CCAGAGACTA TCACTGATTT CTGGAGAATG GTGTGGGAGC AAAGAACCGC CACTATTGTC 
ATGATGACCC GGCTAGAGGA AAAATCAAGG GTGAAATGTG ACCAGTACTG GCCCGGTCGT 
GGCTCAGAGA CCTATGGTAT GATCCAAGTC ACATTAATGG ACACAGTAGA GCTTGCCACC 
TACACAGTTA GGACTTTCAC ACTTTATAAG AATGGCTCCA GTGAGAAGCG GGATGTCAGA 
CAGTTCCAAT TTATGGCCTG GCCTGATCAC GGGGTCCCTG AATATCCTAC TCCTATTCTT 
GGATTCCTTC GCAGAGTGAA AGGCTGTAAT CCACCTGATG CTGGTCCTAT GGTTGTTCAT 
TGCAGTGCTG GCGTGGGCAG AACAGGATGC TTTATTGTTA TTGATGCCAT GCTAGAACGG 
GTGAAACTGG AGAAAACTGT GGATATTTAT GGTCATGTGA CCTGCATGCG CTCCCAACGA 
AATTATATGG TGCAAACAGA GGACCAGTAC ATCTTTATCC ATGAGGCTTT GCTTGAAGCA 
GTAATGTGCG GGATCACTGA AGTGCCAGCA CGCAGTCTGT ATGCACATAT CCAGAAACTG 
TCTCAGGTCC CTCCGGGTGA GAGTGTTACC TCCATGGAAC TAGAGTTCAA GCTTTTGGCC 
AACTCTAAAG CCCACACCTC ACGATTTATC AGTGCCAACC TCCCATGCAA CAAGTTCAAA 
AACAGACTGG TAAACATCAT GCCATATGAA CTGAGTCGTG TCTCCCTGCA GCCCATTCGT 
GGTGTTGAGG GATCAGATTA TATCAATGCA AGTTTCATTG ATGGCTACAG GCAGCAGAAA 
GGCTACATTG CTACTCAGGG TCCATTGGCC GAGACCACTG AGGATTTCTG GAGAATGCTG 
TGGGAACATA ACTCCACCAT TGTGGTGATG CTGACAAAGT TGCGAGAAAT GGGGAGGGAG 
AAGTGCCATC AGTACTGGCC TGCAGAGCGC TCAGCACGGT ACCAGTATTT TGTGGTGGAC 
CCTATGGCTG AGTATAACAT GCCTCAGTAT ATCCTGCGTG AGTTCAAAGT GACAGATGCA 
CGGGATGGCC AATCTCGTAC AATTAGACAA TTCCAGTTCA CTGATTGGCC TGAACAAGGA 
GTGCCAAAAA CAGGTGAAGG CTTTATTGAC TTCATTGGGC AGGTGCACAA AACCAAGGAG 
CAGTTTGGGC AGGATGGGCC AATCACCGTT CACTGCAGCG CTGGCGTGGG CCGCACTGGA 
GTATTCATCA CTCTCAGTAT TGTACTGGAG CGTATGAGGT ACGAGGGTGT GGTTGACATG 
TTTCAAACAG TGAAGACTTT AAGAACACAG CGGCCAGCAA TGGTACAAAC AGAGGACCAG 
TATCAACTGT GCTATAGAGC CGCACTGGAG TATCTGGGCA GCTTTGACCA CTATGCAACA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_031900924.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756784.1 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X3, mRNA.

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CloneID	OXa57129
Clone ID Related Accession (Same CDS sequence)	XM_031900924.1
Accession Version	XM_031900924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAC ACCCTCAGCC CCGCCACAGG ATGTGGAGTG CTCGAGTCTT 
AGCTCCACTG CCATCCGGGT AAGTTGGGTG GCCCCTCCAG CTTCCAGCCG CAACGGGGCC 
CTGACCCGCT ACTCACTTGC CTACCAAGCC CTGGCTGGGG AGGACACATC CCGGCATGTT 
GTGGACAGCA TCTCACCAGA TATCTCCAGC TGGGAGATCA AGGATCTGGA GAAATGGACT 
GAGTACAAAG TATGGGTGCG AGCTCATACA GATGTAGGGG CTGGGCCTGA AAGCTCCCCC 
ATCATTGTGC GGACAGATGA GGATGTGCCG AGTGCACCAC CAAGAAAGGT TGAAGTAGAC 
TCTGTAAACT CCACATCTGT TCGTGTGACA TGGAAGTCTC CTCTACCAAC CAAGCAAAAT 
GGGCAAATTA GGGGGTATCA AGTCACTTAT GTTCGTCTAG AAGGTGGAGA GCCTCGGGGG 
AATCCCATCA TAAAAGATGT TATGCTGTCA GAGGCTCAAT GGAGACCTGA GGATGTGGGA 
GATTACGATG CTGTTATCAC TGGCCTCTCT CCTGAGACCA CTTATTCCAT CACTGTGGCC 
GCCTACACTA CTAAGGGAGA TGGTGCTAGA AGCAAACCCA AGGTCGTTAG CACCACAGGA 
GCTGTGCCTG GTAAACCCAC TATGATGATC AGCACCACAG CACTGAACAC TGCTCTGATC 
CAGTGGCATC CTCCAAGAGA GATTGTGGGA GAGTTGTTAG GATATCGTTT GCAGTACAAG 
CGAGTGGATG AAGACAGGTT TATTACAATT GATTTTAGCA AGAATGAACA TCACCACACA 
GTGACTGGGC TACATAAAGG TGCAAGCTAT ATATTCAAGC TTTCTGCAAA AAACCGAGCT 
GGAGCAGGGG AGGAATATGA AAAGGAAATT TCCACAGCTG AAGATTTTCC TAGTGGGTTT 
CCCCAAAATC TGCAAGTAGT TGGACTTACA ACCTCTACTA CAGAAGTGTC CTGGGAACCA 
CCAGTGTTGT CTGAGCGCAA TGGAAAGATC ACCAACTACA CAGTGGTGTA TCGGGACATA 
AATAGCCAGC AGGATCTCAC CAATGTCACT CGCGACACAC ACATAACATT GGTCAATCTC 
AAGCCAGACA CCACATATGA CATCAAGGTC AGGGCAAGAA CAAACAAAGG CGCTGGCCCT 
CTAAGTCCTA GCATTCAATC ACGTACAATG CCTGTTGACC AAGTGTTTGC TAAGAATTTC 
AGAGTAAACG CCGTGACAAA AACCTCAGTC TTGCTAAGCT GGGAGCTGCC TGATTCTTAC 
AAATCAGCTG TGCCTTTCAA GATCTTTTAC AATAATCAAC ATGTAGAAGT TGATGGACAC 
TCTATGCGCA AACTGATCAG TAATCTCAAG CCAGATACAG AATACTCCTT TGTGCTGATG 
AACAGGGGGA GCAGTGCAGG TGGACTGCAG CACCGCGTGT CCATTCGCAC AGCACCAGAT 
GTTTTAAAAA GCAAGCCTTT TGCTGCCAAC AAATACATGG AGGAGGGCAG GTTCACCTTG 
CTGTTGCCTC AGGTTCAGAC TACTTCCCCA GTAAGGTGGT ATTATATTGT GGTTGTCCCA 
CTGGACAGTT CCAGCGGGGG TCTGAATTCT CGCTGGCAGA GCCCTGAGGA AATGGAGCTA 
GAGCAGATAC TTGAAGCAAT AAACCAGGGA TCCCGTCGTC ATAGAAGACA GGCAAGCAAG 
AATAAGCCAT ACATTGCAGC CCGAGTAAAC ACACTTCCAG AATCCTTTAT TTTGGGTGAC 
AATAAAGAAT ATAATGGGTT CATCAACAAG CCACTGAATC AGAATTTACG CTACACCTGC 
TTTGTGCTAG CTGCACTAGA AGATGGGGAC ACTAAAAAGT ATGCAGCCAG CCCATACTCA 
GATGATATTG TGGTGGAGGT GATCTCACAG GCAAAACAAC AGGAAGAGCC AGAAATGCTT 
TGGGTCATGG GTCCTGTTCT GGCTGTAATT CTTATAATAA TTATAGTTAT TGCCATTCTA 
CTTTTCAAAA GGAAAAGGAC CAACTCTCCT TCAGGCAAAG ATGAACAGTC AGTAGGACTT 
AAAGACTGTC TCCTCGCTCA CTCCTCAGAT CCAGTTGAAA TGAGGCGTCT AAACTACCAA 
ACTCCAGGTT CCAGTGTCCC CAGTTACCCG AATATCTCAA GTATGAGAGA CCACCCTCCT 
ATTCCAATCT CTGACCTGGC AGACAACATT GAAAGACTGA AGGCTAATGA TGGACTCAAG 
TTCTCTCAGG AATATGAGTC TATTGATCCA GGTCAGCAAT TCACGTGGGA AAATTCAAAT 
CTAGAAGTAA ACAAACCAAA GAATCGCTAT GCAAATGTCA TTGCATATGA CCATTCAAGG 
GTCATTCTTA CCTCAGTGGA TGGAGTTCCC GGAAGTGATT ACATTAATGC CAATTACATA 
GATGGGTACA GGAAACAGAA TGCCTATATA GCTACGCAGG GCCCACTGCC AGAGACTATC 
ACTGATTTCT GGAGAATGGT GTGGGAGCAA AGAACCGCCA CTATTGTCAT GATGACCCGG 
CTAGAGGAAA AATCAAGGGT GAAATGTGAC CAGTACTGGC CCGGTCGTGG CTCAGAGACC 
TATGGTATGA TCCAAGTCAC ATTAATGGAC ACAGTAGAGC TTGCCACCTA CACAGTTAGG 
ACTTTCACAC TTTATAAGAA TGGCTCCAGT GAGAAGCGGG ATGTCAGACA GTTCCAATTT 
ATGGCCTGGC CTGATCACGG GGTCCCTGAA TATCCTACTC CTATTCTTGG ATTCCTTCGC 
AGAGTGAAAG GCTGTAATCC ACCTGATGCT GGTCCTATGG TTGTTCATTG CAGTGCTGGC 
GTGGGCAGAA CAGGATGCTT TATTGTTATT GATGCCATGC TAGAACGGGT GAAACTGGAG 
AAAACTGTGG ATATTTATGG TCATGTGACC TGCATGCGCT CCCAACGAAA TTATATGGTG 
CAAACAGAGG ACCAGTACAT CTTTATCCAT GAGGCTTTGC TTGAAGCAGT AATGTGCGGG 
ATCACTGAAG TGCCAGCACG CAGTCTGTAT GCACATATCC AGAAACTGTC TCAGGTCCCT 
CCGGGTGAGA GTGTTACCTC CATGGAACTA GAGTTCAAGC TTTTGGCCAA CTCTAAAGCC 
CACACCTCAC GATTTATCAG TGCCAACCTC CCATGCAACA AGTTCAAAAA CAGACTGGTA 
AACATCATGC CATATGAACT GAGTCGTGTC TCCCTGCAGC CCATTCGTGG TGTTGAGGGA 
TCAGATTATA TCAATGCAAG TTTCATTGAT GGCTACAGGC AGCAGAAAGG CTACATTGCT 
ACTCAGGGTC CATTGGCCGA GACCACTGAG GATTTCTGGA GAATGCTGTG GGAACATAAC 
TCCACCATTG TGGTGATGCT GACAAAGTTG CGAGAAATGG GGAGGGAGAA GTGCCATCAG 
TACTGGCCTG CAGAGCGCTC AGCACGGTAC CAGTATTTTG TGGTGGACCC TATGGCTGAG 
TATAACATGC CTCAGTATAT CCTGCGTGAG TTCAAAGTGA CAGATGCACG GGATGGCCAA 
TCTCGTACAA TTAGACAATT CCAGTTCACT GATTGGCCTG AACAAGGAGT GCCAAAAACA 
GGTGAAGGCT TTATTGACTT CATTGGGCAG GTGCACAAAA CCAAGGAGCA GTTTGGGCAG 
GATGGGCCAA TCACCGTTCA CTGCAGCGCT GGCGTGGGCC GCACTGGAGT ATTCATCACT 
CTCAGTATTG TACTGGAGCG TATGAGGTAC GAGGGTGTGG TTGACATGTT TCAAACAGTG 
AAGACTTTAA GAACACAGCG GCCAGCAATG GTACAAACAG AGGACCAGTA TCAACTGTGC 
TATAGAGCCG CACTGGAGTA TCTGGGCAGC TTTGACCACT ATGCAACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756784.1
CDS	636..6386
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQWRPEDVGDYDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKRKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGSSVPSYPNISSMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_031900924.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900924.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAC ACCCTCAGCC CCGCCACAGG ATGTGGAGTG CTCGAGTCTT 
AGCTCCACTG CCATCCGGGT AAGTTGGGTG GCCCCTCCAG CTTCCAGCCG CAACGGGGCC 
CTGACCCGCT ACTCACTTGC CTACCAAGCC CTGGCTGGGG AGGACACATC CCGGCATGTT 
GTGGACAGCA TCTCACCAGA TATCTCCAGC TGGGAGATCA AGGATCTGGA GAAATGGACT 
GAGTACAAAG TATGGGTGCG AGCTCATACA GATGTAGGGG CTGGGCCTGA AAGCTCCCCC 
ATCATTGTGC GGACAGATGA GGATGTGCCG AGTGCACCAC CAAGAAAGGT TGAAGTAGAC 
TCTGTAAACT CCACATCTGT TCGTGTGACA TGGAAGTCTC CTCTACCAAC CAAGCAAAAT 
GGGCAAATTA GGGGGTATCA AGTCACTTAT GTTCGTCTAG AAGGTGGAGA GCCTCGGGGG 
AATCCCATCA TAAAAGATGT TATGCTGTCA GAGGCTCAAT GGAGACCTGA GGATGTGGGA 
GATTACGATG CTGTTATCAC TGGCCTCTCT CCTGAGACCA CTTATTCCAT CACTGTGGCC 
GCCTACACTA CTAAGGGAGA TGGTGCTAGA AGCAAACCCA AGGTCGTTAG CACCACAGGA 
GCTGTGCCTG GTAAACCCAC TATGATGATC AGCACCACAG CACTGAACAC TGCTCTGATC 
CAGTGGCATC CTCCAAGAGA GATTGTGGGA GAGTTGTTAG GATATCGTTT GCAGTACAAG 
CGAGTGGATG AAGACAGGTT TATTACAATT GATTTTAGCA AGAATGAACA TCACCACACA 
GTGACTGGGC TACATAAAGG TGCAAGCTAT ATATTCAAGC TTTCTGCAAA AAACCGAGCT 
GGAGCAGGGG AGGAATATGA AAAGGAAATT TCCACAGCTG AAGATTTTCC TAGTGGGTTT 
CCCCAAAATC TGCAAGTAGT TGGACTTACA ACCTCTACTA CAGAAGTGTC CTGGGAACCA 
CCAGTGTTGT CTGAGCGCAA TGGAAAGATC ACCAACTACA CAGTGGTGTA TCGGGACATA 
AATAGCCAGC AGGATCTCAC CAATGTCACT CGCGACACAC ACATAACATT GGTCAATCTC 
AAGCCAGACA CCACATATGA CATCAAGGTC AGGGCAAGAA CAAACAAAGG CGCTGGCCCT 
CTAAGTCCTA GCATTCAATC ACGTACAATG CCTGTTGACC AAGTGTTTGC TAAGAATTTC 
AGAGTAAACG CCGTGACAAA AACCTCAGTC TTGCTAAGCT GGGAGCTGCC TGATTCTTAC 
AAATCAGCTG TGCCTTTCAA GATCTTTTAC AATAATCAAC ATGTAGAAGT TGATGGACAC 
TCTATGCGCA AACTGATCAG TAATCTCAAG CCAGATACAG AATACTCCTT TGTGCTGATG 
AACAGGGGGA GCAGTGCAGG TGGACTGCAG CACCGCGTGT CCATTCGCAC AGCACCAGAT 
GTTTTAAAAA GCAAGCCTTT TGCTGCCAAC AAATACATGG AGGAGGGCAG GTTCACCTTG 
CTGTTGCCTC AGGTTCAGAC TACTTCCCCA GTAAGGTGGT ATTATATTGT GGTTGTCCCA 
CTGGACAGTT CCAGCGGGGG TCTGAATTCT CGCTGGCAGA GCCCTGAGGA AATGGAGCTA 
GAGCAGATAC TTGAAGCAAT AAACCAGGGA TCCCGTCGTC ATAGAAGACA GGCAAGCAAG 
AATAAGCCAT ACATTGCAGC CCGAGTAAAC ACACTTCCAG AATCCTTTAT TTTGGGTGAC 
AATAAAGAAT ATAATGGGTT CATCAACAAG CCACTGAATC AGAATTTACG CTACACCTGC 
TTTGTGCTAG CTGCACTAGA AGATGGGGAC ACTAAAAAGT ATGCAGCCAG CCCATACTCA 
GATGATATTG TGGTGGAGGT GATCTCACAG GCAAAACAAC AGGAAGAGCC AGAAATGCTT 
TGGGTCATGG GTCCTGTTCT GGCTGTAATT CTTATAATAA TTATAGTTAT TGCCATTCTA 
CTTTTCAAAA GGAAAAGGAC CAACTCTCCT TCAGGCAAAG ATGAACAGTC AGTAGGACTT 
AAAGACTGTC TCCTCGCTCA CTCCTCAGAT CCAGTTGAAA TGAGGCGTCT AAACTACCAA 
ACTCCAGGTT CCAGTGTCCC CAGTTACCCG AATATCTCAA GTATGAGAGA CCACCCTCCT 
ATTCCAATCT CTGACCTGGC AGACAACATT GAAAGACTGA AGGCTAATGA TGGACTCAAG 
TTCTCTCAGG AATATGAGTC TATTGATCCA GGTCAGCAAT TCACGTGGGA AAATTCAAAT 
CTAGAAGTAA ACAAACCAAA GAATCGCTAT GCAAATGTCA TTGCATATGA CCATTCAAGG 
GTCATTCTTA CCTCAGTGGA TGGAGTTCCC GGAAGTGATT ACATTAATGC CAATTACATA 
GATGGGTACA GGAAACAGAA TGCCTATATA GCTACGCAGG GCCCACTGCC AGAGACTATC 
ACTGATTTCT GGAGAATGGT GTGGGAGCAA AGAACCGCCA CTATTGTCAT GATGACCCGG 
CTAGAGGAAA AATCAAGGGT GAAATGTGAC CAGTACTGGC CCGGTCGTGG CTCAGAGACC 
TATGGTATGA TCCAAGTCAC ATTAATGGAC ACAGTAGAGC TTGCCACCTA CACAGTTAGG 
ACTTTCACAC TTTATAAGAA TGGCTCCAGT GAGAAGCGGG ATGTCAGACA GTTCCAATTT 
ATGGCCTGGC CTGATCACGG GGTCCCTGAA TATCCTACTC CTATTCTTGG ATTCCTTCGC 
AGAGTGAAAG GCTGTAATCC ACCTGATGCT GGTCCTATGG TTGTTCATTG CAGTGCTGGC 
GTGGGCAGAA CAGGATGCTT TATTGTTATT GATGCCATGC TAGAACGGGT GAAACTGGAG 
AAAACTGTGG ATATTTATGG TCATGTGACC TGCATGCGCT CCCAACGAAA TTATATGGTG 
CAAACAGAGG ACCAGTACAT CTTTATCCAT GAGGCTTTGC TTGAAGCAGT AATGTGCGGG 
ATCACTGAAG TGCCAGCACG CAGTCTGTAT GCACATATCC AGAAACTGTC TCAGGTCCCT 
CCGGGTGAGA GTGTTACCTC CATGGAACTA GAGTTCAAGC TTTTGGCCAA CTCTAAAGCC 
CACACCTCAC GATTTATCAG TGCCAACCTC CCATGCAACA AGTTCAAAAA CAGACTGGTA 
AACATCATGC CATATGAACT GAGTCGTGTC TCCCTGCAGC CCATTCGTGG TGTTGAGGGA 
TCAGATTATA TCAATGCAAG TTTCATTGAT GGCTACAGGC AGCAGAAAGG CTACATTGCT 
ACTCAGGGTC CATTGGCCGA GACCACTGAG GATTTCTGGA GAATGCTGTG GGAACATAAC 
TCCACCATTG TGGTGATGCT GACAAAGTTG CGAGAAATGG GGAGGGAGAA GTGCCATCAG 
TACTGGCCTG CAGAGCGCTC AGCACGGTAC CAGTATTTTG TGGTGGACCC TATGGCTGAG 
TATAACATGC CTCAGTATAT CCTGCGTGAG TTCAAAGTGA CAGATGCACG GGATGGCCAA 
TCTCGTACAA TTAGACAATT CCAGTTCACT GATTGGCCTG AACAAGGAGT GCCAAAAACA 
GGTGAAGGCT TTATTGACTT CATTGGGCAG GTGCACAAAA CCAAGGAGCA GTTTGGGCAG 
GATGGGCCAA TCACCGTTCA CTGCAGCGCT GGCGTGGGCC GCACTGGAGT ATTCATCACT 
CTCAGTATTG TACTGGAGCG TATGAGGTAC GAGGGTGTGG TTGACATGTT TCAAACAGTG 
AAGACTTTAA GAACACAGCG GCCAGCAATG GTACAAACAG AGGACCAGTA TCAACTGTGC 
TATAGAGCCG CACTGGAGTA TCTGGGCAGC TTTGACCACT ATGCAACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_031900925.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756785.1 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X4, mRNA.

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CloneID	OXa57130
Clone ID Related Accession (Same CDS sequence)	XM_031900925.1
Accession Version	XM_031900925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTGAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACACT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGACATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCGAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTGACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTTCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA ATGGAGACCT GAGGATGTGG GAGATTACGA TGCTGTTATC 
ACTGGCCTCT CTCCTGAGAC CACTTATTCC ATCACTGTGG CCGCCTACAC TACTAAGGGA 
GATGGTGCTA GAAGCAAACC CAAGGTCGTT AGCACCACAG GAGCTGTGCC TGGTAAACCC 
ACTATGATGA TCAGCACCAC AGCACTGAAC ACTGCTCTGA TCCAGTGGCA TCCTCCAAGA 
GAGATTGTGG GAGAGTTGTT AGGATATCGT TTGCAGTACA AGCGAGTGGA TGAAGACAGG 
TTTATTACAA TTGATTTTAG CAAGAATGAA CATCACCACA CAGTGACTGG GCTACATAAA 
GGTGCAAGCT ATATATTCAA GCTTTCTGCA AAAAACCGAG CTGGAGCAGG GGAGGAATAT 
GAAAAGGAAA TTTCCACAGC TGAAGATTTT CCTAGTGGGT TTCCCCAAAA TCTGCAAGTA 
GTTGGACTTA CAACCTCTAC TACAGAAGTG TCCTGGGAAC CACCAGTGTT GTCTGAGCGC 
AATGGAAAGA TCACCAACTA CACAGTGGTG TATCGGGACA TAAATAGCCA GCAGGATCTC 
ACCAATGTCA CTCGCGACAC ACACATAACA TTGGTCAATC TCAAGCCAGA CACCACATAT 
GACATCAAGG TCAGGGCAAG AACAAACAAA GGCGCTGGCC CTCTAAGTCC TAGCATTCAA 
TCACGTACAA TGCCTGTTGA CCAAGTGTTT GCTAAGAATT TCAGAGTAAA CGCCGTGACA 
AAAACCTCAG TCTTGCTAAG CTGGGAGCTG CCTGATTCTT ACAAATCAGC TGTGCCTTTC 
AAGATCTTTT ACAATAATCA ACATGTAGAA GTTGATGGAC ACTCTATGCG CAAACTGATC 
AGTAATCTCA AGCCAGATAC AGAATACTCC TTTGTGCTGA TGAACAGGGG GAGCAGTGCA 
GGTGGACTGC AGCACCGCGT GTCCATTCGC ACAGCACCAG ATGTTTTAAA AAGCAAGCCT 
TTTGCTGCCA ACAAATACAT GGAGGAGGGC AGGTTCACCT TGCTGTTGCC TCAGGTTCAG 
ACTACTTCCC CAGTAAGGTG GTATTATATT GTGGTTGTCC CACTGGACAG TTCCAGCGGG 
GGTCTGAATT CTCGCTGGCA GAGCCCTGAG GAAATGGAGC TAGAGCAGAT ACTTGAAGCA 
ATAAACCAGG GATCCCGTCG TCATAGAAGA CAGGCAAGCA AGAATAAGCC ATACATTGCA 
GCCCGAGTAA ACACACTTCC AGAATCCTTT ATTTTGGGTG ACAATAAAGA ATATAATGGG 
TTCATCAACA AGCCACTGAA TCAGAATTTA CGCTACACCT GCTTTGTGCT AGCTGCACTA 
GAAGATGGGG ACACTAAAAA GTATGCAGCC AGCCCATACT CAGATGATAT TGTGGTGGAG 
GTGATCTCAC AGGCAAAACA ACAGGAAGAG CCAGAAATGC TTTGGGTCAT GGGTCCTGTT 
CTGGCTGTAA TTCTTATAAT AATTATAGTT ATTGCCATTC TACTTTTCAA AAGTAAACAG 
GAAAGGAAAA GGACCAACTC TCCTTCAGGC AAAGATGAAC AGTCAGTAGG ACTTAAAGAC 
TGTCTCCTCG CTCACTCCTC AGATCCAGTT GAAATGAGGC GTCTAAACTA CCAAACTCCA 
GGTTCCAGTG TCCCCAGTTA CCCGAATATC TCAAGTATGA GAGACCACCC TCCTATTCCA 
ATCTCTGACC TGGCAGACAA CATTGAAAGA CTGAAGGCTA ATGATGGACT CAAGTTCTCT 
CAGGAATATG AGTCTATTGA TCCAGGTCAG CAATTCACGT GGGAAAATTC AAATCTAGAA 
GTAAACAAAC CAAAGAATCG CTATGCAAAT GTCATTGCAT ATGACCATTC AAGGGTCATT 
CTTACCTCAG TGGATGGAGT TCCCGGAAGT GATTACATTA ATGCCAATTA CATAGATGGG 
TACAGGAAAC AGAATGCCTA TATAGCTACG CAGGGCCCAC TGCCAGAGAC TATCACTGAT 
TTCTGGAGAA TGGTGTGGGA GCAAAGAACC GCCACTATTG TCATGATGAC CCGGCTAGAG 
GAAAAATCAA GGGTGAAATG TGACCAGTAC TGGCCCGGTC GTGGCTCAGA GACCTATGGT 
ATGATCCAAG TCACATTAAT GGACACAGTA GAGCTTGCCA CCTACACAGT TAGGACTTTC 
ACACTTTATA AGAATGGCTC CAGTGAGAAG CGGGATGTCA GACAGTTCCA ATTTATGGCC 
TGGCCTGATC ACGGGGTCCC TGAATATCCT ACTCCTATTC TTGGATTCCT TCGCAGAGTG 
AAAGGCTGTA ATCCACCTGA TGCTGGTCCT ATGGTTGTTC ATTGCAGTGC TGGCGTGGGC 
AGAACAGGAT GCTTTATTGT TATTGATGCC ATGCTAGAAC GGGTGAAACT GGAGAAAACT 
GTGGATATTT ATGGTCATGT GACCTGCATG CGCTCCCAAC GAAATTATAT GGTGCAAACA 
GAGGACCAGT ACATCTTTAT CCATGAGGCT TTGCTTGAAG CAGTAATGTG CGGGATCACT 
GAAGTGCCAG CACGCAGTCT GTATGCACAT ATCCAGAAAC TGTCTCAGGT CCCTCCGGGT 
GAGAGTGTTA CCTCCATGGA ACTAGAGTTC AAGCTTTTGG CCAACTCTAA AGCCCACACC 
TCACGATTTA TCAGTGCCAA CCTCCCATGC AACAAGTTCA AAAACAGACT GGTAAACATC 
ATGCCATATG AACTGAGTCG TGTCTCCCTG CAGCCCATTC GTGGTGTTGA GGGATCAGAT 
TATATCAATG CAAGTTTCAT TGATGGCTAC AGGCAGCAGA AAGGCTACAT TGCTACTCAG 
GGTCCATTGG CCGAGACCAC TGAGGATTTC TGGAGAATGC TGTGGGAACA TAACTCCACC 
ATTGTGGTGA TGCTGACAAA GTTGCGAGAA ATGGGGAGGG AGAAGTGCCA TCAGTACTGG 
CCTGCAGAGC GCTCAGCACG GTACCAGTAT TTTGTGGTGG ACCCTATGGC TGAGTATAAC 
ATGCCTCAGT ATATCCTGCG TGAGTTCAAA GTGACAGATG CACGGGATGG CCAATCTCGT 
ACAATTAGAC AATTCCAGTT CACTGATTGG CCTGAACAAG GAGTGCCAAA AACAGGTGAA 
GGCTTTATTG ACTTCATTGG GCAGGTGCAC AAAACCAAGG AGCAGTTTGG GCAGGATGGG 
CCAATCACCG TTCACTGCAG CGCTGGCGTG GGCCGCACTG GAGTATTCAT CACTCTCAGT 
ATTGTACTGG AGCGTATGAG GTACGAGGGT GTGGTTGACA TGTTTCAAAC AGTGAAGACT 
TTAAGAACAC AGCGGCCAGC AATGGTACAA ACAGAGGACC AGTATCAACT GTGCTATAGA 
GCCGCACTGG AGTATCTGGG CAGCTTTGAC CACTATGCAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756785.1
CDS	636..6380
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQWRPEDVGDYDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKSKQERKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGSSVPSYPNISSMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_031900925.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900925.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTGAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACACT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGACATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCGAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTGACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTTCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA ATGGAGACCT GAGGATGTGG GAGATTACGA TGCTGTTATC 
ACTGGCCTCT CTCCTGAGAC CACTTATTCC ATCACTGTGG CCGCCTACAC TACTAAGGGA 
GATGGTGCTA GAAGCAAACC CAAGGTCGTT AGCACCACAG GAGCTGTGCC TGGTAAACCC 
ACTATGATGA TCAGCACCAC AGCACTGAAC ACTGCTCTGA TCCAGTGGCA TCCTCCAAGA 
GAGATTGTGG GAGAGTTGTT AGGATATCGT TTGCAGTACA AGCGAGTGGA TGAAGACAGG 
TTTATTACAA TTGATTTTAG CAAGAATGAA CATCACCACA CAGTGACTGG GCTACATAAA 
GGTGCAAGCT ATATATTCAA GCTTTCTGCA AAAAACCGAG CTGGAGCAGG GGAGGAATAT 
GAAAAGGAAA TTTCCACAGC TGAAGATTTT CCTAGTGGGT TTCCCCAAAA TCTGCAAGTA 
GTTGGACTTA CAACCTCTAC TACAGAAGTG TCCTGGGAAC CACCAGTGTT GTCTGAGCGC 
AATGGAAAGA TCACCAACTA CACAGTGGTG TATCGGGACA TAAATAGCCA GCAGGATCTC 
ACCAATGTCA CTCGCGACAC ACACATAACA TTGGTCAATC TCAAGCCAGA CACCACATAT 
GACATCAAGG TCAGGGCAAG AACAAACAAA GGCGCTGGCC CTCTAAGTCC TAGCATTCAA 
TCACGTACAA TGCCTGTTGA CCAAGTGTTT GCTAAGAATT TCAGAGTAAA CGCCGTGACA 
AAAACCTCAG TCTTGCTAAG CTGGGAGCTG CCTGATTCTT ACAAATCAGC TGTGCCTTTC 
AAGATCTTTT ACAATAATCA ACATGTAGAA GTTGATGGAC ACTCTATGCG CAAACTGATC 
AGTAATCTCA AGCCAGATAC AGAATACTCC TTTGTGCTGA TGAACAGGGG GAGCAGTGCA 
GGTGGACTGC AGCACCGCGT GTCCATTCGC ACAGCACCAG ATGTTTTAAA AAGCAAGCCT 
TTTGCTGCCA ACAAATACAT GGAGGAGGGC AGGTTCACCT TGCTGTTGCC TCAGGTTCAG 
ACTACTTCCC CAGTAAGGTG GTATTATATT GTGGTTGTCC CACTGGACAG TTCCAGCGGG 
GGTCTGAATT CTCGCTGGCA GAGCCCTGAG GAAATGGAGC TAGAGCAGAT ACTTGAAGCA 
ATAAACCAGG GATCCCGTCG TCATAGAAGA CAGGCAAGCA AGAATAAGCC ATACATTGCA 
GCCCGAGTAA ACACACTTCC AGAATCCTTT ATTTTGGGTG ACAATAAAGA ATATAATGGG 
TTCATCAACA AGCCACTGAA TCAGAATTTA CGCTACACCT GCTTTGTGCT AGCTGCACTA 
GAAGATGGGG ACACTAAAAA GTATGCAGCC AGCCCATACT CAGATGATAT TGTGGTGGAG 
GTGATCTCAC AGGCAAAACA ACAGGAAGAG CCAGAAATGC TTTGGGTCAT GGGTCCTGTT 
CTGGCTGTAA TTCTTATAAT AATTATAGTT ATTGCCATTC TACTTTTCAA AAGTAAACAG 
GAAAGGAAAA GGACCAACTC TCCTTCAGGC AAAGATGAAC AGTCAGTAGG ACTTAAAGAC 
TGTCTCCTCG CTCACTCCTC AGATCCAGTT GAAATGAGGC GTCTAAACTA CCAAACTCCA 
GGTTCCAGTG TCCCCAGTTA CCCGAATATC TCAAGTATGA GAGACCACCC TCCTATTCCA 
ATCTCTGACC TGGCAGACAA CATTGAAAGA CTGAAGGCTA ATGATGGACT CAAGTTCTCT 
CAGGAATATG AGTCTATTGA TCCAGGTCAG CAATTCACGT GGGAAAATTC AAATCTAGAA 
GTAAACAAAC CAAAGAATCG CTATGCAAAT GTCATTGCAT ATGACCATTC AAGGGTCATT 
CTTACCTCAG TGGATGGAGT TCCCGGAAGT GATTACATTA ATGCCAATTA CATAGATGGG 
TACAGGAAAC AGAATGCCTA TATAGCTACG CAGGGCCCAC TGCCAGAGAC TATCACTGAT 
TTCTGGAGAA TGGTGTGGGA GCAAAGAACC GCCACTATTG TCATGATGAC CCGGCTAGAG 
GAAAAATCAA GGGTGAAATG TGACCAGTAC TGGCCCGGTC GTGGCTCAGA GACCTATGGT 
ATGATCCAAG TCACATTAAT GGACACAGTA GAGCTTGCCA CCTACACAGT TAGGACTTTC 
ACACTTTATA AGAATGGCTC CAGTGAGAAG CGGGATGTCA GACAGTTCCA ATTTATGGCC 
TGGCCTGATC ACGGGGTCCC TGAATATCCT ACTCCTATTC TTGGATTCCT TCGCAGAGTG 
AAAGGCTGTA ATCCACCTGA TGCTGGTCCT ATGGTTGTTC ATTGCAGTGC TGGCGTGGGC 
AGAACAGGAT GCTTTATTGT TATTGATGCC ATGCTAGAAC GGGTGAAACT GGAGAAAACT 
GTGGATATTT ATGGTCATGT GACCTGCATG CGCTCCCAAC GAAATTATAT GGTGCAAACA 
GAGGACCAGT ACATCTTTAT CCATGAGGCT TTGCTTGAAG CAGTAATGTG CGGGATCACT 
GAAGTGCCAG CACGCAGTCT GTATGCACAT ATCCAGAAAC TGTCTCAGGT CCCTCCGGGT 
GAGAGTGTTA CCTCCATGGA ACTAGAGTTC AAGCTTTTGG CCAACTCTAA AGCCCACACC 
TCACGATTTA TCAGTGCCAA CCTCCCATGC AACAAGTTCA AAAACAGACT GGTAAACATC 
ATGCCATATG AACTGAGTCG TGTCTCCCTG CAGCCCATTC GTGGTGTTGA GGGATCAGAT 
TATATCAATG CAAGTTTCAT TGATGGCTAC AGGCAGCAGA AAGGCTACAT TGCTACTCAG 
GGTCCATTGG CCGAGACCAC TGAGGATTTC TGGAGAATGC TGTGGGAACA TAACTCCACC 
ATTGTGGTGA TGCTGACAAA GTTGCGAGAA ATGGGGAGGG AGAAGTGCCA TCAGTACTGG 
CCTGCAGAGC GCTCAGCACG GTACCAGTAT TTTGTGGTGG ACCCTATGGC TGAGTATAAC 
ATGCCTCAGT ATATCCTGCG TGAGTTCAAA GTGACAGATG CACGGGATGG CCAATCTCGT 
ACAATTAGAC AATTCCAGTT CACTGATTGG CCTGAACAAG GAGTGCCAAA AACAGGTGAA 
GGCTTTATTG ACTTCATTGG GCAGGTGCAC AAAACCAAGG AGCAGTTTGG GCAGGATGGG 
CCAATCACCG TTCACTGCAG CGCTGGCGTG GGCCGCACTG GAGTATTCAT CACTCTCAGT 
ATTGTACTGG AGCGTATGAG GTACGAGGGT GTGGTTGACA TGTTTCAAAC AGTGAAGACT 
TTAAGAACAC AGCGGCCAGC AATGGTACAA ACAGAGGACC AGTATCAACT GTGCTATAGA 
GCCGCACTGG AGTATCTGGG CAGCTTTGAC CACTATGCAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_031900926.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756786.1 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X5, mRNA.

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CloneID	OXa57131
Clone ID Related Accession (Same CDS sequence)	XM_031900926.1
Accession Version	XM_031900926.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAC ACCCTCAGCC CCGCCACAGG ATGTGGAGTG CTCGAGTCTT 
AGCTCCACTG CCATCCGGGT AAGTTGGGTG GCCCCTCCAG CTTCCAGCCG CAACGGGGCC 
CTGACCCGCT ACTCACTTGC CTACCAAGCC CTGGCTGGGG AGGACACATC CCGGCATGTT 
GTGGACAGCA TCTCACCAGA TATCTCCAGC TGGGAGATCA AGGATCTGGA GAAATGGACT 
GAGTACAAAG TATGGGTGCG AGCTCATACA GATGTAGGGG CTGGGCCTGA AAGCTCCCCC 
ATCATTGTGC GGACAGATGA GGATGTGCCG AGTGCACCAC CAAGAAAGGT TGAAGTAGAC 
TCTGTAAACT CCACATCTGT TCGTGTGACA TGGAAGTCTC CTCTACCAAC CAAGCAAAAT 
GGGCAAATTA GGGGGTATCA AGTCACTTAT GTTCGTCTAG AAGGTGGAGA GCCTCGGGGG 
AATCCCATCA TAAAAGATGT TATGCTGTCA GAGGCTCAAG ATGCTGTTAT CACTGGCCTC 
TCTCCTGAGA CCACTTATTC CATCACTGTG GCCGCCTACA CTACTAAGGG AGATGGTGCT 
AGAAGCAAAC CCAAGGTCGT TAGCACCACA GGAGCTGTGC CTGGTAAACC CACTATGATG 
ATCAGCACCA CAGCACTGAA CACTGCTCTG ATCCAGTGGC ATCCTCCAAG AGAGATTGTG 
GGAGAGTTGT TAGGATATCG TTTGCAGTAC AAGCGAGTGG ATGAAGACAG GTTTATTACA 
ATTGATTTTA GCAAGAATGA ACATCACCAC ACAGTGACTG GGCTACATAA AGGTGCAAGC 
TATATATTCA AGCTTTCTGC AAAAAACCGA GCTGGAGCAG GGGAGGAATA TGAAAAGGAA 
ATTTCCACAG CTGAAGATTT TCCTAGTGGG TTTCCCCAAA ATCTGCAAGT AGTTGGACTT 
ACAACCTCTA CTACAGAAGT GTCCTGGGAA CCACCAGTGT TGTCTGAGCG CAATGGAAAG 
ATCACCAACT ACACAGTGGT GTATCGGGAC ATAAATAGCC AGCAGGATCT CACCAATGTC 
ACTCGCGACA CACACATAAC ATTGGTCAAT CTCAAGCCAG ACACCACATA TGACATCAAG 
GTCAGGGCAA GAACAAACAA AGGCGCTGGC CCTCTAAGTC CTAGCATTCA ATCACGTACA 
ATGCCTGTTG ACCAAGTGTT TGCTAAGAAT TTCAGAGTAA ACGCCGTGAC AAAAACCTCA 
GTCTTGCTAA GCTGGGAGCT GCCTGATTCT TACAAATCAG CTGTGCCTTT CAAGATCTTT 
TACAATAATC AACATGTAGA AGTTGATGGA CACTCTATGC GCAAACTGAT CAGTAATCTC 
AAGCCAGATA CAGAATACTC CTTTGTGCTG ATGAACAGGG GGAGCAGTGC AGGTGGACTG 
CAGCACCGCG TGTCCATTCG CACAGCACCA GATGTTTTAA AAAGCAAGCC TTTTGCTGCC 
AACAAATACA TGGAGGAGGG CAGGTTCACC TTGCTGTTGC CTCAGGTTCA GACTACTTCC 
CCAGTAAGGT GGTATTATAT TGTGGTTGTC CCACTGGACA GTTCCAGCGG GGGTCTGAAT 
TCTCGCTGGC AGAGCCCTGA GGAAATGGAG CTAGAGCAGA TACTTGAAGC AATAAACCAG 
GGATCCCGTC GTCATAGAAG ACAGGCAAGC AAGAATAAGC CATACATTGC AGCCCGAGTA 
AACACACTTC CAGAATCCTT TATTTTGGGT GACAATAAAG AATATAATGG GTTCATCAAC 
AAGCCACTGA ATCAGAATTT ACGCTACACC TGCTTTGTGC TAGCTGCACT AGAAGATGGG 
GACACTAAAA AGTATGCAGC CAGCCCATAC TCAGATGATA TTGTGGTGGA GGTGATCTCA 
CAGGCAAAAC AACAGGAAGA GCCAGAAATG CTTTGGGTCA TGGGTCCTGT TCTGGCTGTA 
ATTCTTATAA TAATTATAGT TATTGCCATT CTACTTTTCA AAAGTAAACA GGAAAGGAAA 
AGGACCAACT CTCCTTCAGG CAAAGATGAA CAGTCAGTAG GACTTAAAGA CTGTCTCCTC 
GCTCACTCCT CAGATCCAGT TGAAATGAGG CGTCTAAACT ACCAAACTCC AGGTTCCAGT 
GTCCCCAGTT ACCCGAATAT CTCAAGTATG AGAGACCACC CTCCTATTCC AATCTCTGAC 
CTGGCAGACA ACATTGAAAG ACTGAAGGCT AATGATGGAC TCAAGTTCTC TCAGGAATAT 
GAGTCTATTG ATCCAGGTCA GCAATTCACG TGGGAAAATT CAAATCTAGA AGTAAACAAA 
CCAAAGAATC GCTATGCAAA TGTCATTGCA TATGACCATT CAAGGGTCAT TCTTACCTCA 
GTGGATGGAG TTCCCGGAAG TGATTACATT AATGCCAATT ACATAGATGG GTACAGGAAA 
CAGAATGCCT ATATAGCTAC GCAGGGCCCA CTGCCAGAGA CTATCACTGA TTTCTGGAGA 
ATGGTGTGGG AGCAAAGAAC CGCCACTATT GTCATGATGA CCCGGCTAGA GGAAAAATCA 
AGGGTGAAAT GTGACCAGTA CTGGCCCGGT CGTGGCTCAG AGACCTATGG TATGATCCAA 
GTCACATTAA TGGACACAGT AGAGCTTGCC ACCTACACAG TTAGGACTTT CACACTTTAT 
AAGAATGGCT CCAGTGAGAA GCGGGATGTC AGACAGTTCC AATTTATGGC CTGGCCTGAT 
CACGGGGTCC CTGAATATCC TACTCCTATT CTTGGATTCC TTCGCAGAGT GAAAGGCTGT 
AATCCACCTG ATGCTGGTCC TATGGTTGTT CATTGCAGTG CTGGCGTGGG CAGAACAGGA 
TGCTTTATTG TTATTGATGC CATGCTAGAA CGGGTGAAAC TGGAGAAAAC TGTGGATATT 
TATGGTCATG TGACCTGCAT GCGCTCCCAA CGAAATTATA TGGTGCAAAC AGAGGACCAG 
TACATCTTTA TCCATGAGGC TTTGCTTGAA GCAGTAATGT GCGGGATCAC TGAAGTGCCA 
GCACGCAGTC TGTATGCACA TATCCAGAAA CTGTCTCAGG TCCCTCCGGG TGAGAGTGTT 
ACCTCCATGG AACTAGAGTT CAAGCTTTTG GCCAACTCTA AAGCCCACAC CTCACGATTT 
ATCAGTGCCA ACCTCCCATG CAACAAGTTC AAAAACAGAC TGGTAAACAT CATGCCATAT 
GAACTGAGTC GTGTCTCCCT GCAGCCCATT CGTGGTGTTG AGGGATCAGA TTATATCAAT 
GCAAGTTTCA TTGATGGCTA CAGGCAGCAG AAAGGCTACA TTGCTACTCA GGGTCCATTG 
GCCGAGACCA CTGAGGATTT CTGGAGAATG CTGTGGGAAC ATAACTCCAC CATTGTGGTG 
ATGCTGACAA AGTTGCGAGA AATGGGGAGG GAGAAGTGCC ATCAGTACTG GCCTGCAGAG 
CGCTCAGCAC GGTACCAGTA TTTTGTGGTG GACCCTATGG CTGAGTATAA CATGCCTCAG 
TATATCCTGC GTGAGTTCAA AGTGACAGAT GCACGGGATG GCCAATCTCG TACAATTAGA 
CAATTCCAGT TCACTGATTG GCCTGAACAA GGAGTGCCAA AAACAGGTGA AGGCTTTATT 
GACTTCATTG GGCAGGTGCA CAAAACCAAG GAGCAGTTTG GGCAGGATGG GCCAATCACC 
GTTCACTGCA GCGCTGGCGT GGGCCGCACT GGAGTATTCA TCACTCTCAG TATTGTACTG 
GAGCGTATGA GGTACGAGGG TGTGGTTGAC ATGTTTCAAA CAGTGAAGAC TTTAAGAACA 
CAGCGGCCAG CAATGGTACA AACAGAGGAC CAGTATCAAC TGTGCTATAG AGCCGCACTG 
GAGTATCTGG GCAGCTTTGA CCACTATGCA ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756786.1
CDS	636..6371
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKSKQERKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGSSVPSYPNISSMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_031900926.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900926.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAC ACCCTCAGCC CCGCCACAGG ATGTGGAGTG CTCGAGTCTT 
AGCTCCACTG CCATCCGGGT AAGTTGGGTG GCCCCTCCAG CTTCCAGCCG CAACGGGGCC 
CTGACCCGCT ACTCACTTGC CTACCAAGCC CTGGCTGGGG AGGACACATC CCGGCATGTT 
GTGGACAGCA TCTCACCAGA TATCTCCAGC TGGGAGATCA AGGATCTGGA GAAATGGACT 
GAGTACAAAG TATGGGTGCG AGCTCATACA GATGTAGGGG CTGGGCCTGA AAGCTCCCCC 
ATCATTGTGC GGACAGATGA GGATGTGCCG AGTGCACCAC CAAGAAAGGT TGAAGTAGAC 
TCTGTAAACT CCACATCTGT TCGTGTGACA TGGAAGTCTC CTCTACCAAC CAAGCAAAAT 
GGGCAAATTA GGGGGTATCA AGTCACTTAT GTTCGTCTAG AAGGTGGAGA GCCTCGGGGG 
AATCCCATCA TAAAAGATGT TATGCTGTCA GAGGCTCAAG ATGCTGTTAT CACTGGCCTC 
TCTCCTGAGA CCACTTATTC CATCACTGTG GCCGCCTACA CTACTAAGGG AGATGGTGCT 
AGAAGCAAAC CCAAGGTCGT TAGCACCACA GGAGCTGTGC CTGGTAAACC CACTATGATG 
ATCAGCACCA CAGCACTGAA CACTGCTCTG ATCCAGTGGC ATCCTCCAAG AGAGATTGTG 
GGAGAGTTGT TAGGATATCG TTTGCAGTAC AAGCGAGTGG ATGAAGACAG GTTTATTACA 
ATTGATTTTA GCAAGAATGA ACATCACCAC ACAGTGACTG GGCTACATAA AGGTGCAAGC 
TATATATTCA AGCTTTCTGC AAAAAACCGA GCTGGAGCAG GGGAGGAATA TGAAAAGGAA 
ATTTCCACAG CTGAAGATTT TCCTAGTGGG TTTCCCCAAA ATCTGCAAGT AGTTGGACTT 
ACAACCTCTA CTACAGAAGT GTCCTGGGAA CCACCAGTGT TGTCTGAGCG CAATGGAAAG 
ATCACCAACT ACACAGTGGT GTATCGGGAC ATAAATAGCC AGCAGGATCT CACCAATGTC 
ACTCGCGACA CACACATAAC ATTGGTCAAT CTCAAGCCAG ACACCACATA TGACATCAAG 
GTCAGGGCAA GAACAAACAA AGGCGCTGGC CCTCTAAGTC CTAGCATTCA ATCACGTACA 
ATGCCTGTTG ACCAAGTGTT TGCTAAGAAT TTCAGAGTAA ACGCCGTGAC AAAAACCTCA 
GTCTTGCTAA GCTGGGAGCT GCCTGATTCT TACAAATCAG CTGTGCCTTT CAAGATCTTT 
TACAATAATC AACATGTAGA AGTTGATGGA CACTCTATGC GCAAACTGAT CAGTAATCTC 
AAGCCAGATA CAGAATACTC CTTTGTGCTG ATGAACAGGG GGAGCAGTGC AGGTGGACTG 
CAGCACCGCG TGTCCATTCG CACAGCACCA GATGTTTTAA AAAGCAAGCC TTTTGCTGCC 
AACAAATACA TGGAGGAGGG CAGGTTCACC TTGCTGTTGC CTCAGGTTCA GACTACTTCC 
CCAGTAAGGT GGTATTATAT TGTGGTTGTC CCACTGGACA GTTCCAGCGG GGGTCTGAAT 
TCTCGCTGGC AGAGCCCTGA GGAAATGGAG CTAGAGCAGA TACTTGAAGC AATAAACCAG 
GGATCCCGTC GTCATAGAAG ACAGGCAAGC AAGAATAAGC CATACATTGC AGCCCGAGTA 
AACACACTTC CAGAATCCTT TATTTTGGGT GACAATAAAG AATATAATGG GTTCATCAAC 
AAGCCACTGA ATCAGAATTT ACGCTACACC TGCTTTGTGC TAGCTGCACT AGAAGATGGG 
GACACTAAAA AGTATGCAGC CAGCCCATAC TCAGATGATA TTGTGGTGGA GGTGATCTCA 
CAGGCAAAAC AACAGGAAGA GCCAGAAATG CTTTGGGTCA TGGGTCCTGT TCTGGCTGTA 
ATTCTTATAA TAATTATAGT TATTGCCATT CTACTTTTCA AAAGTAAACA GGAAAGGAAA 
AGGACCAACT CTCCTTCAGG CAAAGATGAA CAGTCAGTAG GACTTAAAGA CTGTCTCCTC 
GCTCACTCCT CAGATCCAGT TGAAATGAGG CGTCTAAACT ACCAAACTCC AGGTTCCAGT 
GTCCCCAGTT ACCCGAATAT CTCAAGTATG AGAGACCACC CTCCTATTCC AATCTCTGAC 
CTGGCAGACA ACATTGAAAG ACTGAAGGCT AATGATGGAC TCAAGTTCTC TCAGGAATAT 
GAGTCTATTG ATCCAGGTCA GCAATTCACG TGGGAAAATT CAAATCTAGA AGTAAACAAA 
CCAAAGAATC GCTATGCAAA TGTCATTGCA TATGACCATT CAAGGGTCAT TCTTACCTCA 
GTGGATGGAG TTCCCGGAAG TGATTACATT AATGCCAATT ACATAGATGG GTACAGGAAA 
CAGAATGCCT ATATAGCTAC GCAGGGCCCA CTGCCAGAGA CTATCACTGA TTTCTGGAGA 
ATGGTGTGGG AGCAAAGAAC CGCCACTATT GTCATGATGA CCCGGCTAGA GGAAAAATCA 
AGGGTGAAAT GTGACCAGTA CTGGCCCGGT CGTGGCTCAG AGACCTATGG TATGATCCAA 
GTCACATTAA TGGACACAGT AGAGCTTGCC ACCTACACAG TTAGGACTTT CACACTTTAT 
AAGAATGGCT CCAGTGAGAA GCGGGATGTC AGACAGTTCC AATTTATGGC CTGGCCTGAT 
CACGGGGTCC CTGAATATCC TACTCCTATT CTTGGATTCC TTCGCAGAGT GAAAGGCTGT 
AATCCACCTG ATGCTGGTCC TATGGTTGTT CATTGCAGTG CTGGCGTGGG CAGAACAGGA 
TGCTTTATTG TTATTGATGC CATGCTAGAA CGGGTGAAAC TGGAGAAAAC TGTGGATATT 
TATGGTCATG TGACCTGCAT GCGCTCCCAA CGAAATTATA TGGTGCAAAC AGAGGACCAG 
TACATCTTTA TCCATGAGGC TTTGCTTGAA GCAGTAATGT GCGGGATCAC TGAAGTGCCA 
GCACGCAGTC TGTATGCACA TATCCAGAAA CTGTCTCAGG TCCCTCCGGG TGAGAGTGTT 
ACCTCCATGG AACTAGAGTT CAAGCTTTTG GCCAACTCTA AAGCCCACAC CTCACGATTT 
ATCAGTGCCA ACCTCCCATG CAACAAGTTC AAAAACAGAC TGGTAAACAT CATGCCATAT 
GAACTGAGTC GTGTCTCCCT GCAGCCCATT CGTGGTGTTG AGGGATCAGA TTATATCAAT 
GCAAGTTTCA TTGATGGCTA CAGGCAGCAG AAAGGCTACA TTGCTACTCA GGGTCCATTG 
GCCGAGACCA CTGAGGATTT CTGGAGAATG CTGTGGGAAC ATAACTCCAC CATTGTGGTG 
ATGCTGACAA AGTTGCGAGA AATGGGGAGG GAGAAGTGCC ATCAGTACTG GCCTGCAGAG 
CGCTCAGCAC GGTACCAGTA TTTTGTGGTG GACCCTATGG CTGAGTATAA CATGCCTCAG 
TATATCCTGC GTGAGTTCAA AGTGACAGAT GCACGGGATG GCCAATCTCG TACAATTAGA 
CAATTCCAGT TCACTGATTG GCCTGAACAA GGAGTGCCAA AAACAGGTGA AGGCTTTATT 
GACTTCATTG GGCAGGTGCA CAAAACCAAG GAGCAGTTTG GGCAGGATGG GCCAATCACC 
GTTCACTGCA GCGCTGGCGT GGGCCGCACT GGAGTATTCA TCACTCTCAG TATTGTACTG 
GAGCGTATGA GGTACGAGGG TGTGGTTGAC ATGTTTCAAA CAGTGAAGAC TTTAAGAACA 
CAGCGGCCAG CAATGGTACA AACAGAGGAC CAGTATCAAC TGTGCTATAG AGCCGCACTG 
GAGTATCTGG GCAGCTTTGA CCACTATGCA ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_031900927.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756787.1 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X6, mRNA.

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CloneID	OXa57132
Clone ID Related Accession (Same CDS sequence)	XM_031900927.1
Accession Version	XM_031900927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAC ACCCTCAGCC CCGCCACAGG ATGTGGAGTG CTCGAGTCTT 
AGCTCCACTG CCATCCGGGT AAGTTGGGTG GCCCCTCCAG CTTCCAGCCG CAACGGGGCC 
CTGACCCGCT ACTCACTTGC CTACCAAGCC CTGGCTGGGG AGGACACATC CCGGCATGTT 
GTGGACAGCA TCTCACCAGA TATCTCCAGC TGGGAGATCA AGGATCTGGA GAAATGGACT 
GAGTACAAAG TATGGGTGCG AGCTCATACA GATGTAGGGG CTGGGCCTGA AAGCTCCCCC 
ATCATTGTGC GGACAGATGA GGATGTGCCG AGTGCACCAC CAAGAAAGGT TGAAGTAGAC 
TCTGTAAACT CCACATCTGT TCGTGTGACA TGGAAGTCTC CTCTACCAAC CAAGCAAAAT 
GGGCAAATTA GGGGGTATCA AGTCACTTAT GTTCGTCTAG AAGGTGGAGA GCCTCGGGGG 
AATCCCATCA TAAAAGATGT TATGCTGTCA GAGGCTCAAT GGAGACCTGA GGATGTGGGA 
GATTACGATG CTGTTATCAC TGGCCTCTCT CCTGAGACCA CTTATTCCAT CACTGTGGCC 
GCCTACACTA CTAAGGGAGA TGGTGCTAGA AGCAAACCCA AGGTCGTTAG CACCACAGGA 
GCTGTGCCTG GTAAACCCAC TATGATGATC AGCACCACAG CACTGAACAC TGCTCTGATC 
CAGTGGCATC CTCCAAGAGA GATTGTGGGA GAGTTGTTAG GATATCGTTT GCAGTACAAG 
CGAGTGGATG AAGACAGGTT TATTACAATT GATTTTAGCA AGAATGAACA TCACCACACA 
GTGACTGGGC TACATAAAGG TGCAAGCTAT ATATTCAAGC TTTCTGCAAA AAACCGAGCT 
GGAGCAGGGG AGGAATATGA AAAGGAAATT TCCACAGCTG AAGATTTTCC TAGTGGGTTT 
CCCCAAAATC TGCAAGTAGT TGGACTTACA ACCTCTACTA CAGAAGTGTC CTGGGAACCA 
CCAGTGTTGT CTGAGCGCAA TGGAAAGATC ACCAACTACA CAGTGGTGTA TCGGGACATA 
AATAGCCAGC AGGATCTCAC CAATGTCACT CGCGACACAC ACATAACATT GGTCAATCTC 
AAGCCAGACA CCACATATGA CATCAAGGTC AGGGCAAGAA CAAACAAAGG CGCTGGCCCT 
CTAAGTCCTA GCATTCAATC ACGTACAATG CCTGTTGACC AAGTGTTTGC TAAGAATTTC 
AGAGTAAACG CCGTGACAAA AACCTCAGTC TTGCTAAGCT GGGAGCTGCC TGATTCTTAC 
AAATCAGCTG TGCCTTTCAA GATCTTTTAC AATAATCAAC ATGTAGAAGT TGATGGACAC 
TCTATGCGCA AACTGATCAG TAATCTCAAG CCAGATACAG AATACTCCTT TGTGCTGATG 
AACAGGGGGA GCAGTGCAGG TGGACTGCAG CACCGCGTGT CCATTCGCAC AGCACCAGAT 
GTTTTAAAAA GCAAGCCTTT TGCTGCCAAC AAATACATGG AGGAGGGCAG GTTCACCTTG 
CTGTTGCCTC AGGTTCAGAC TACTTCCCCA GTAAGGTGGT ATTATATTGT GGTTGTCCCA 
CTGGACAGTT CCAGCGGGGG TCTGAATTCT CGCTGGCAGA GCCCTGAGGA AATGGAGCTA 
GAGCAGATAC TTGAAGCAAT AAACCAGGGA TCCCGTCGTC ATAGAAGACA GGCAAGCAAG 
AATAAGCCAT ACATTGCAGC CCGAGTAAAC ACACTTCCAG AATCCTTTAT TTTGGGTGAC 
AATAAAGAAT ATAATGGGTT CATCAACAAG CCACTGAATC AGAATTTACG CTACACCTGC 
TTTGTGCTAG CTGCACTAGA AGATGGGGAC ACTAAAAAGT ATGCAGCCAG CCCATACTCA 
GATGATATTG TGGTGGAGGT GATCTCACAG GCAAAACAAC AGGAAGAGCC AGAAATGCTT 
TGGGTCATGG GTCCTGTTCT GGCTGTAATT CTTATAATAA TTATAGTTAT TGCCATTCTA 
CTTTTCAAAA GTAAACAGGA AAGGAAAAGG ACCAACTCTC CTTCAGGCAA AGATGAACAG 
TCAGTAGGAC TTAAAGACTG TCTCCTCGCT CACTCCTCAG ATCCAGTTGA AATGAGGCGT 
CTAAACTACC AAACTCCAGG TATGAGAGAC CACCCTCCTA TTCCAATCTC TGACCTGGCA 
GACAACATTG AAAGACTGAA GGCTAATGAT GGACTCAAGT TCTCTCAGGA ATATGAGTCT 
ATTGATCCAG GTCAGCAATT CACGTGGGAA AATTCAAATC TAGAAGTAAA CAAACCAAAG 
AATCGCTATG CAAATGTCAT TGCATATGAC CATTCAAGGG TCATTCTTAC CTCAGTGGAT 
GGAGTTCCCG GAAGTGATTA CATTAATGCC AATTACATAG ATGGGTACAG GAAACAGAAT 
GCCTATATAG CTACGCAGGG CCCACTGCCA GAGACTATCA CTGATTTCTG GAGAATGGTG 
TGGGAGCAAA GAACCGCCAC TATTGTCATG ATGACCCGGC TAGAGGAAAA ATCAAGGGTG 
AAATGTGACC AGTACTGGCC CGGTCGTGGC TCAGAGACCT ATGGTATGAT CCAAGTCACA 
TTAATGGACA CAGTAGAGCT TGCCACCTAC ACAGTTAGGA CTTTCACACT TTATAAGAAT 
GGCTCCAGTG AGAAGCGGGA TGTCAGACAG TTCCAATTTA TGGCCTGGCC TGATCACGGG 
GTCCCTGAAT ATCCTACTCC TATTCTTGGA TTCCTTCGCA GAGTGAAAGG CTGTAATCCA 
CCTGATGCTG GTCCTATGGT TGTTCATTGC AGTGCTGGCG TGGGCAGAAC AGGATGCTTT 
ATTGTTATTG ATGCCATGCT AGAACGGGTG AAACTGGAGA AAACTGTGGA TATTTATGGT 
CATGTGACCT GCATGCGCTC CCAACGAAAT TATATGGTGC AAACAGAGGA CCAGTACATC 
TTTATCCATG AGGCTTTGCT TGAAGCAGTA ATGTGCGGGA TCACTGAAGT GCCAGCACGC 
AGTCTGTATG CACATATCCA GAAACTGTCT CAGGTCCCTC CGGGTGAGAG TGTTACCTCC 
ATGGAACTAG AGTTCAAGCT TTTGGCCAAC TCTAAAGCCC ACACCTCACG ATTTATCAGT 
GCCAACCTCC CATGCAACAA GTTCAAAAAC AGACTGGTAA ACATCATGCC ATATGAACTG 
AGTCGTGTCT CCCTGCAGCC CATTCGTGGT GTTGAGGGAT CAGATTATAT CAATGCAAGT 
TTCATTGATG GCTACAGGCA GCAGAAAGGC TACATTGCTA CTCAGGGTCC ATTGGCCGAG 
ACCACTGAGG ATTTCTGGAG AATGCTGTGG GAACATAACT CCACCATTGT GGTGATGCTG 
ACAAAGTTGC GAGAAATGGG GAGGGAGAAG TGCCATCAGT ACTGGCCTGC AGAGCGCTCA 
GCACGGTACC AGTATTTTGT GGTGGACCCT ATGGCTGAGT ATAACATGCC TCAGTATATC 
CTGCGTGAGT TCAAAGTGAC AGATGCACGG GATGGCCAAT CTCGTACAAT TAGACAATTC 
CAGTTCACTG ATTGGCCTGA ACAAGGAGTG CCAAAAACAG GTGAAGGCTT TATTGACTTC 
ATTGGGCAGG TGCACAAAAC CAAGGAGCAG TTTGGGCAGG ATGGGCCAAT CACCGTTCAC 
TGCAGCGCTG GCGTGGGCCG CACTGGAGTA TTCATCACTC TCAGTATTGT ACTGGAGCGT 
ATGAGGTACG AGGGTGTGGT TGACATGTTT CAAACAGTGA AGACTTTAAG AACACAGCGG 
CCAGCAATGG TACAAACAGA GGACCAGTAT CAACTGTGCT ATAGAGCCGC ACTGGAGTAT 
CTGGGCAGCT TTGACCACTA TGCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756787.1
CDS	636..6365
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQWRPEDVGDYDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKSKQERKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_031900927.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900927.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAC ACCCTCAGCC CCGCCACAGG ATGTGGAGTG CTCGAGTCTT 
AGCTCCACTG CCATCCGGGT AAGTTGGGTG GCCCCTCCAG CTTCCAGCCG CAACGGGGCC 
CTGACCCGCT ACTCACTTGC CTACCAAGCC CTGGCTGGGG AGGACACATC CCGGCATGTT 
GTGGACAGCA TCTCACCAGA TATCTCCAGC TGGGAGATCA AGGATCTGGA GAAATGGACT 
GAGTACAAAG TATGGGTGCG AGCTCATACA GATGTAGGGG CTGGGCCTGA AAGCTCCCCC 
ATCATTGTGC GGACAGATGA GGATGTGCCG AGTGCACCAC CAAGAAAGGT TGAAGTAGAC 
TCTGTAAACT CCACATCTGT TCGTGTGACA TGGAAGTCTC CTCTACCAAC CAAGCAAAAT 
GGGCAAATTA GGGGGTATCA AGTCACTTAT GTTCGTCTAG AAGGTGGAGA GCCTCGGGGG 
AATCCCATCA TAAAAGATGT TATGCTGTCA GAGGCTCAAT GGAGACCTGA GGATGTGGGA 
GATTACGATG CTGTTATCAC TGGCCTCTCT CCTGAGACCA CTTATTCCAT CACTGTGGCC 
GCCTACACTA CTAAGGGAGA TGGTGCTAGA AGCAAACCCA AGGTCGTTAG CACCACAGGA 
GCTGTGCCTG GTAAACCCAC TATGATGATC AGCACCACAG CACTGAACAC TGCTCTGATC 
CAGTGGCATC CTCCAAGAGA GATTGTGGGA GAGTTGTTAG GATATCGTTT GCAGTACAAG 
CGAGTGGATG AAGACAGGTT TATTACAATT GATTTTAGCA AGAATGAACA TCACCACACA 
GTGACTGGGC TACATAAAGG TGCAAGCTAT ATATTCAAGC TTTCTGCAAA AAACCGAGCT 
GGAGCAGGGG AGGAATATGA AAAGGAAATT TCCACAGCTG AAGATTTTCC TAGTGGGTTT 
CCCCAAAATC TGCAAGTAGT TGGACTTACA ACCTCTACTA CAGAAGTGTC CTGGGAACCA 
CCAGTGTTGT CTGAGCGCAA TGGAAAGATC ACCAACTACA CAGTGGTGTA TCGGGACATA 
AATAGCCAGC AGGATCTCAC CAATGTCACT CGCGACACAC ACATAACATT GGTCAATCTC 
AAGCCAGACA CCACATATGA CATCAAGGTC AGGGCAAGAA CAAACAAAGG CGCTGGCCCT 
CTAAGTCCTA GCATTCAATC ACGTACAATG CCTGTTGACC AAGTGTTTGC TAAGAATTTC 
AGAGTAAACG CCGTGACAAA AACCTCAGTC TTGCTAAGCT GGGAGCTGCC TGATTCTTAC 
AAATCAGCTG TGCCTTTCAA GATCTTTTAC AATAATCAAC ATGTAGAAGT TGATGGACAC 
TCTATGCGCA AACTGATCAG TAATCTCAAG CCAGATACAG AATACTCCTT TGTGCTGATG 
AACAGGGGGA GCAGTGCAGG TGGACTGCAG CACCGCGTGT CCATTCGCAC AGCACCAGAT 
GTTTTAAAAA GCAAGCCTTT TGCTGCCAAC AAATACATGG AGGAGGGCAG GTTCACCTTG 
CTGTTGCCTC AGGTTCAGAC TACTTCCCCA GTAAGGTGGT ATTATATTGT GGTTGTCCCA 
CTGGACAGTT CCAGCGGGGG TCTGAATTCT CGCTGGCAGA GCCCTGAGGA AATGGAGCTA 
GAGCAGATAC TTGAAGCAAT AAACCAGGGA TCCCGTCGTC ATAGAAGACA GGCAAGCAAG 
AATAAGCCAT ACATTGCAGC CCGAGTAAAC ACACTTCCAG AATCCTTTAT TTTGGGTGAC 
AATAAAGAAT ATAATGGGTT CATCAACAAG CCACTGAATC AGAATTTACG CTACACCTGC 
TTTGTGCTAG CTGCACTAGA AGATGGGGAC ACTAAAAAGT ATGCAGCCAG CCCATACTCA 
GATGATATTG TGGTGGAGGT GATCTCACAG GCAAAACAAC AGGAAGAGCC AGAAATGCTT 
TGGGTCATGG GTCCTGTTCT GGCTGTAATT CTTATAATAA TTATAGTTAT TGCCATTCTA 
CTTTTCAAAA GTAAACAGGA AAGGAAAAGG ACCAACTCTC CTTCAGGCAA AGATGAACAG 
TCAGTAGGAC TTAAAGACTG TCTCCTCGCT CACTCCTCAG ATCCAGTTGA AATGAGGCGT 
CTAAACTACC AAACTCCAGG TATGAGAGAC CACCCTCCTA TTCCAATCTC TGACCTGGCA 
GACAACATTG AAAGACTGAA GGCTAATGAT GGACTCAAGT TCTCTCAGGA ATATGAGTCT 
ATTGATCCAG GTCAGCAATT CACGTGGGAA AATTCAAATC TAGAAGTAAA CAAACCAAAG 
AATCGCTATG CAAATGTCAT TGCATATGAC CATTCAAGGG TCATTCTTAC CTCAGTGGAT 
GGAGTTCCCG GAAGTGATTA CATTAATGCC AATTACATAG ATGGGTACAG GAAACAGAAT 
GCCTATATAG CTACGCAGGG CCCACTGCCA GAGACTATCA CTGATTTCTG GAGAATGGTG 
TGGGAGCAAA GAACCGCCAC TATTGTCATG ATGACCCGGC TAGAGGAAAA ATCAAGGGTG 
AAATGTGACC AGTACTGGCC CGGTCGTGGC TCAGAGACCT ATGGTATGAT CCAAGTCACA 
TTAATGGACA CAGTAGAGCT TGCCACCTAC ACAGTTAGGA CTTTCACACT TTATAAGAAT 
GGCTCCAGTG AGAAGCGGGA TGTCAGACAG TTCCAATTTA TGGCCTGGCC TGATCACGGG 
GTCCCTGAAT ATCCTACTCC TATTCTTGGA TTCCTTCGCA GAGTGAAAGG CTGTAATCCA 
CCTGATGCTG GTCCTATGGT TGTTCATTGC AGTGCTGGCG TGGGCAGAAC AGGATGCTTT 
ATTGTTATTG ATGCCATGCT AGAACGGGTG AAACTGGAGA AAACTGTGGA TATTTATGGT 
CATGTGACCT GCATGCGCTC CCAACGAAAT TATATGGTGC AAACAGAGGA CCAGTACATC 
TTTATCCATG AGGCTTTGCT TGAAGCAGTA ATGTGCGGGA TCACTGAAGT GCCAGCACGC 
AGTCTGTATG CACATATCCA GAAACTGTCT CAGGTCCCTC CGGGTGAGAG TGTTACCTCC 
ATGGAACTAG AGTTCAAGCT TTTGGCCAAC TCTAAAGCCC ACACCTCACG ATTTATCAGT 
GCCAACCTCC CATGCAACAA GTTCAAAAAC AGACTGGTAA ACATCATGCC ATATGAACTG 
AGTCGTGTCT CCCTGCAGCC CATTCGTGGT GTTGAGGGAT CAGATTATAT CAATGCAAGT 
TTCATTGATG GCTACAGGCA GCAGAAAGGC TACATTGCTA CTCAGGGTCC ATTGGCCGAG 
ACCACTGAGG ATTTCTGGAG AATGCTGTGG GAACATAACT CCACCATTGT GGTGATGCTG 
ACAAAGTTGC GAGAAATGGG GAGGGAGAAG TGCCATCAGT ACTGGCCTGC AGAGCGCTCA 
GCACGGTACC AGTATTTTGT GGTGGACCCT ATGGCTGAGT ATAACATGCC TCAGTATATC 
CTGCGTGAGT TCAAAGTGAC AGATGCACGG GATGGCCAAT CTCGTACAAT TAGACAATTC 
CAGTTCACTG ATTGGCCTGA ACAAGGAGTG CCAAAAACAG GTGAAGGCTT TATTGACTTC 
ATTGGGCAGG TGCACAAAAC CAAGGAGCAG TTTGGGCAGG ATGGGCCAAT CACCGTTCAC 
TGCAGCGCTG GCGTGGGCCG CACTGGAGTA TTCATCACTC TCAGTATTGT ACTGGAGCGT 
ATGAGGTACG AGGGTGTGGT TGACATGTTT CAAACAGTGA AGACTTTAAG AACACAGCGG 
CCAGCAATGG TACAAACAGA GGACCAGTAT CAACTGTGCT ATAGAGCCGC ACTGGAGTAT 
CTGGGCAGCT TTGACCACTA TGCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_031900928.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756788.1 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X7, mRNA.

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CloneID	OXa57133
Clone ID Related Accession (Same CDS sequence)	XM_031900928.1
Accession Version	XM_031900928.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAC ACCCTCAGCC CCGCCACAGG ATGTGGAGTG CTCGAGTCTT 
AGCTCCACTG CCATCCGGGT AAGTTGGGTG GCCCCTCCAG CTTCCAGCCG CAACGGGGCC 
CTGACCCGCT ACTCACTTGC CTACCAAGCC CTGGCTGGGG AGGACACATC CCGGCATGTT 
GTGGACAGCA TCTCACCAGA TATCTCCAGC TGGGAGATCA AGGATCTGGA GAAATGGACT 
GAGTACAAAG TATGGGTGCG AGCTCATACA GATGTAGGGG CTGGGCCTGA AAGCTCCCCC 
ATCATTGTGC GGACAGATGA GGATGTGCCG AGTGCACCAC CAAGAAAGGT TGAAGTAGAC 
TCTGTAAACT CCACATCTGT TCGTGTGACA TGGAAGTCTC CTCTACCAAC CAAGCAAAAT 
GGGCAAATTA GGGGGTATCA AGTCACTTAT GTTCGTCTAG AAGGTGGAGA GCCTCGGGGG 
AATCCCATCA TAAAAGATGT TATGCTGTCA GAGGCTCAAT GGAGACCTGA GGATGTGGGA 
GATTACGATG CTGTTATCAC TGGCCTCTCT CCTGAGACCA CTTATTCCAT CACTGTGGCC 
GCCTACACTA CTAAGGGAGA TGGTGCTAGA AGCAAACCCA AGGTCGTTAG CACCACAGGA 
GCTGTGCCTG GTAAACCCAC TATGATGATC AGCACCACAG CACTGAACAC TGCTCTGATC 
CAGTGGCATC CTCCAAGAGA GATTGTGGGA GAGTTGTTAG GATATCGTTT GCAGTACAAG 
CGAGTGGATG AAGACAGGTT TATTACAATT GATTTTAGCA AGAATGAACA TCACCACACA 
GTGACTGGGC TACATAAAGG TGCAAGCTAT ATATTCAAGC TTTCTGCAAA AAACCGAGCT 
GGAGCAGGGG AGGAATATGA AAAGGAAATT TCCACAGCTG AAGATTTTCC TAGTGGGTTT 
CCCCAAAATC TGCAAGTAGT TGGACTTACA ACCTCTACTA CAGAAGTGTC CTGGGAACCA 
CCAGTGTTGT CTGAGCGCAA TGGAAAGATC ACCAACTACA CAGTGGTGTA TCGGGACATA 
AATAGCCAGC AGGATCTCAC CAATGTCACT CGCGACACAC ACATAACATT GGTCAATCTC 
AAGCCAGACA CCACATATGA CATCAAGGTC AGGGCAAGAA CAAACAAAGG CGCTGGCCCT 
CTAAGTCCTA GCATTCAATC ACGTACAATG CCTGTTGACC AAGTGTTTGC TAAGAATTTC 
AGAGTAAACG CCGTGACAAA AACCTCAGTC TTGCTAAGCT GGGAGCTGCC TGATTCTTAC 
AAATCAGCTG TGCCTTTCAA GATCTTTTAC AATAATCAAC ATGTAGAAGT TGATGGACAC 
TCTATGCGCA AACTGATCAG TAATCTCAAG CCAGATACAG AATACTCCTT TGTGCTGATG 
AACAGGGGGA GCAGTGCAGG TGGACTGCAG CACCGCGTGT CCATTCGCAC AGCACCAGAT 
GTTTTAAAAA GCAAGCCTTT TGCTGCCAAC AAATACATGG AGGAGGGCAG GTTCACCTTG 
CTGTTGCCTC AGGTTCAGAC TACTTCCCCA GTAAGGTGGT ATTATATTGT GGTTGTCCCA 
CTGGACAGTT CCAGCGGGGG TCTGAATTCT CGCTGGCAGA GCCCTGAGGA AATGGAGCTA 
GAGCAGATAC TTGAAGCAAT AAACCAGGGA TCCCGTCGTC ATAGAAGACA GGCAAGCAAG 
AATAAGCCAT ACATTGCAGC CCGAGTAAAC ACACTTCCAG AATCCTTTAT TTTGGGTGAC 
AATAAAGAAT ATAATGGGTT CATCAACAAG CCACTGAATC AGAATTTACG CTACACCTGC 
TTTGTGCTAG CTGCACTAGA AGATGGGGAC ACTAAAAAGT ATGCAGCCAG CCCATACTCA 
GATGATATTG TGGTGGAGGT GATCTCACAG GCAAAACAAC AGGAAGAGCC AGAAATGCTT 
TGGGTCATGG GTCCTGTTCT GGCTGTAATT CTTATAATAA TTATAGTTAT TGCCATTCTA 
CTTTTCAAAA GGAAAAGGAC CAACTCTCCT TCAGGCAAAG ATGAACAGTC AGTAGGACTT 
AAAGACTGTC TCCTCGCTCA CTCCTCAGAT CCAGTTGAAA TGAGGCGTCT AAACTACCAA 
ACTCCAGGTA TGAGAGACCA CCCTCCTATT CCAATCTCTG ACCTGGCAGA CAACATTGAA 
AGACTGAAGG CTAATGATGG ACTCAAGTTC TCTCAGGAAT ATGAGTCTAT TGATCCAGGT 
CAGCAATTCA CGTGGGAAAA TTCAAATCTA GAAGTAAACA AACCAAAGAA TCGCTATGCA 
AATGTCATTG CATATGACCA TTCAAGGGTC ATTCTTACCT CAGTGGATGG AGTTCCCGGA 
AGTGATTACA TTAATGCCAA TTACATAGAT GGGTACAGGA AACAGAATGC CTATATAGCT 
ACGCAGGGCC CACTGCCAGA GACTATCACT GATTTCTGGA GAATGGTGTG GGAGCAAAGA 
ACCGCCACTA TTGTCATGAT GACCCGGCTA GAGGAAAAAT CAAGGGTGAA ATGTGACCAG 
TACTGGCCCG GTCGTGGCTC AGAGACCTAT GGTATGATCC AAGTCACATT AATGGACACA 
GTAGAGCTTG CCACCTACAC AGTTAGGACT TTCACACTTT ATAAGAATGG CTCCAGTGAG 
AAGCGGGATG TCAGACAGTT CCAATTTATG GCCTGGCCTG ATCACGGGGT CCCTGAATAT 
CCTACTCCTA TTCTTGGATT CCTTCGCAGA GTGAAAGGCT GTAATCCACC TGATGCTGGT 
CCTATGGTTG TTCATTGCAG TGCTGGCGTG GGCAGAACAG GATGCTTTAT TGTTATTGAT 
GCCATGCTAG AACGGGTGAA ACTGGAGAAA ACTGTGGATA TTTATGGTCA TGTGACCTGC 
ATGCGCTCCC AACGAAATTA TATGGTGCAA ACAGAGGACC AGTACATCTT TATCCATGAG 
GCTTTGCTTG AAGCAGTAAT GTGCGGGATC ACTGAAGTGC CAGCACGCAG TCTGTATGCA 
CATATCCAGA AACTGTCTCA GGTCCCTCCG GGTGAGAGTG TTACCTCCAT GGAACTAGAG 
TTCAAGCTTT TGGCCAACTC TAAAGCCCAC ACCTCACGAT TTATCAGTGC CAACCTCCCA 
TGCAACAAGT TCAAAAACAG ACTGGTAAAC ATCATGCCAT ATGAACTGAG TCGTGTCTCC 
CTGCAGCCCA TTCGTGGTGT TGAGGGATCA GATTATATCA ATGCAAGTTT CATTGATGGC 
TACAGGCAGC AGAAAGGCTA CATTGCTACT CAGGGTCCAT TGGCCGAGAC CACTGAGGAT 
TTCTGGAGAA TGCTGTGGGA ACATAACTCC ACCATTGTGG TGATGCTGAC AAAGTTGCGA 
GAAATGGGGA GGGAGAAGTG CCATCAGTAC TGGCCTGCAG AGCGCTCAGC ACGGTACCAG 
TATTTTGTGG TGGACCCTAT GGCTGAGTAT AACATGCCTC AGTATATCCT GCGTGAGTTC 
AAAGTGACAG ATGCACGGGA TGGCCAATCT CGTACAATTA GACAATTCCA GTTCACTGAT 
TGGCCTGAAC AAGGAGTGCC AAAAACAGGT GAAGGCTTTA TTGACTTCAT TGGGCAGGTG 
CACAAAACCA AGGAGCAGTT TGGGCAGGAT GGGCCAATCA CCGTTCACTG CAGCGCTGGC 
GTGGGCCGCA CTGGAGTATT CATCACTCTC AGTATTGTAC TGGAGCGTAT GAGGTACGAG 
GGTGTGGTTG ACATGTTTCA AACAGTGAAG ACTTTAAGAA CACAGCGGCC AGCAATGGTA 
CAAACAGAGG ACCAGTATCA ACTGTGCTAT AGAGCCGCAC TGGAGTATCT GGGCAGCTTT 
GACCACTATG CAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756788.1
CDS	636..6353
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQWRPEDVGDYDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKRKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_031900928.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900928.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAC ACCCTCAGCC CCGCCACAGG ATGTGGAGTG CTCGAGTCTT 
AGCTCCACTG CCATCCGGGT AAGTTGGGTG GCCCCTCCAG CTTCCAGCCG CAACGGGGCC 
CTGACCCGCT ACTCACTTGC CTACCAAGCC CTGGCTGGGG AGGACACATC CCGGCATGTT 
GTGGACAGCA TCTCACCAGA TATCTCCAGC TGGGAGATCA AGGATCTGGA GAAATGGACT 
GAGTACAAAG TATGGGTGCG AGCTCATACA GATGTAGGGG CTGGGCCTGA AAGCTCCCCC 
ATCATTGTGC GGACAGATGA GGATGTGCCG AGTGCACCAC CAAGAAAGGT TGAAGTAGAC 
TCTGTAAACT CCACATCTGT TCGTGTGACA TGGAAGTCTC CTCTACCAAC CAAGCAAAAT 
GGGCAAATTA GGGGGTATCA AGTCACTTAT GTTCGTCTAG AAGGTGGAGA GCCTCGGGGG 
AATCCCATCA TAAAAGATGT TATGCTGTCA GAGGCTCAAT GGAGACCTGA GGATGTGGGA 
GATTACGATG CTGTTATCAC TGGCCTCTCT CCTGAGACCA CTTATTCCAT CACTGTGGCC 
GCCTACACTA CTAAGGGAGA TGGTGCTAGA AGCAAACCCA AGGTCGTTAG CACCACAGGA 
GCTGTGCCTG GTAAACCCAC TATGATGATC AGCACCACAG CACTGAACAC TGCTCTGATC 
CAGTGGCATC CTCCAAGAGA GATTGTGGGA GAGTTGTTAG GATATCGTTT GCAGTACAAG 
CGAGTGGATG AAGACAGGTT TATTACAATT GATTTTAGCA AGAATGAACA TCACCACACA 
GTGACTGGGC TACATAAAGG TGCAAGCTAT ATATTCAAGC TTTCTGCAAA AAACCGAGCT 
GGAGCAGGGG AGGAATATGA AAAGGAAATT TCCACAGCTG AAGATTTTCC TAGTGGGTTT 
CCCCAAAATC TGCAAGTAGT TGGACTTACA ACCTCTACTA CAGAAGTGTC CTGGGAACCA 
CCAGTGTTGT CTGAGCGCAA TGGAAAGATC ACCAACTACA CAGTGGTGTA TCGGGACATA 
AATAGCCAGC AGGATCTCAC CAATGTCACT CGCGACACAC ACATAACATT GGTCAATCTC 
AAGCCAGACA CCACATATGA CATCAAGGTC AGGGCAAGAA CAAACAAAGG CGCTGGCCCT 
CTAAGTCCTA GCATTCAATC ACGTACAATG CCTGTTGACC AAGTGTTTGC TAAGAATTTC 
AGAGTAAACG CCGTGACAAA AACCTCAGTC TTGCTAAGCT GGGAGCTGCC TGATTCTTAC 
AAATCAGCTG TGCCTTTCAA GATCTTTTAC AATAATCAAC ATGTAGAAGT TGATGGACAC 
TCTATGCGCA AACTGATCAG TAATCTCAAG CCAGATACAG AATACTCCTT TGTGCTGATG 
AACAGGGGGA GCAGTGCAGG TGGACTGCAG CACCGCGTGT CCATTCGCAC AGCACCAGAT 
GTTTTAAAAA GCAAGCCTTT TGCTGCCAAC AAATACATGG AGGAGGGCAG GTTCACCTTG 
CTGTTGCCTC AGGTTCAGAC TACTTCCCCA GTAAGGTGGT ATTATATTGT GGTTGTCCCA 
CTGGACAGTT CCAGCGGGGG TCTGAATTCT CGCTGGCAGA GCCCTGAGGA AATGGAGCTA 
GAGCAGATAC TTGAAGCAAT AAACCAGGGA TCCCGTCGTC ATAGAAGACA GGCAAGCAAG 
AATAAGCCAT ACATTGCAGC CCGAGTAAAC ACACTTCCAG AATCCTTTAT TTTGGGTGAC 
AATAAAGAAT ATAATGGGTT CATCAACAAG CCACTGAATC AGAATTTACG CTACACCTGC 
TTTGTGCTAG CTGCACTAGA AGATGGGGAC ACTAAAAAGT ATGCAGCCAG CCCATACTCA 
GATGATATTG TGGTGGAGGT GATCTCACAG GCAAAACAAC AGGAAGAGCC AGAAATGCTT 
TGGGTCATGG GTCCTGTTCT GGCTGTAATT CTTATAATAA TTATAGTTAT TGCCATTCTA 
CTTTTCAAAA GGAAAAGGAC CAACTCTCCT TCAGGCAAAG ATGAACAGTC AGTAGGACTT 
AAAGACTGTC TCCTCGCTCA CTCCTCAGAT CCAGTTGAAA TGAGGCGTCT AAACTACCAA 
ACTCCAGGTA TGAGAGACCA CCCTCCTATT CCAATCTCTG ACCTGGCAGA CAACATTGAA 
AGACTGAAGG CTAATGATGG ACTCAAGTTC TCTCAGGAAT ATGAGTCTAT TGATCCAGGT 
CAGCAATTCA CGTGGGAAAA TTCAAATCTA GAAGTAAACA AACCAAAGAA TCGCTATGCA 
AATGTCATTG CATATGACCA TTCAAGGGTC ATTCTTACCT CAGTGGATGG AGTTCCCGGA 
AGTGATTACA TTAATGCCAA TTACATAGAT GGGTACAGGA AACAGAATGC CTATATAGCT 
ACGCAGGGCC CACTGCCAGA GACTATCACT GATTTCTGGA GAATGGTGTG GGAGCAAAGA 
ACCGCCACTA TTGTCATGAT GACCCGGCTA GAGGAAAAAT CAAGGGTGAA ATGTGACCAG 
TACTGGCCCG GTCGTGGCTC AGAGACCTAT GGTATGATCC AAGTCACATT AATGGACACA 
GTAGAGCTTG CCACCTACAC AGTTAGGACT TTCACACTTT ATAAGAATGG CTCCAGTGAG 
AAGCGGGATG TCAGACAGTT CCAATTTATG GCCTGGCCTG ATCACGGGGT CCCTGAATAT 
CCTACTCCTA TTCTTGGATT CCTTCGCAGA GTGAAAGGCT GTAATCCACC TGATGCTGGT 
CCTATGGTTG TTCATTGCAG TGCTGGCGTG GGCAGAACAG GATGCTTTAT TGTTATTGAT 
GCCATGCTAG AACGGGTGAA ACTGGAGAAA ACTGTGGATA TTTATGGTCA TGTGACCTGC 
ATGCGCTCCC AACGAAATTA TATGGTGCAA ACAGAGGACC AGTACATCTT TATCCATGAG 
GCTTTGCTTG AAGCAGTAAT GTGCGGGATC ACTGAAGTGC CAGCACGCAG TCTGTATGCA 
CATATCCAGA AACTGTCTCA GGTCCCTCCG GGTGAGAGTG TTACCTCCAT GGAACTAGAG 
TTCAAGCTTT TGGCCAACTC TAAAGCCCAC ACCTCACGAT TTATCAGTGC CAACCTCCCA 
TGCAACAAGT TCAAAAACAG ACTGGTAAAC ATCATGCCAT ATGAACTGAG TCGTGTCTCC 
CTGCAGCCCA TTCGTGGTGT TGAGGGATCA GATTATATCA ATGCAAGTTT CATTGATGGC 
TACAGGCAGC AGAAAGGCTA CATTGCTACT CAGGGTCCAT TGGCCGAGAC CACTGAGGAT 
TTCTGGAGAA TGCTGTGGGA ACATAACTCC ACCATTGTGG TGATGCTGAC AAAGTTGCGA 
GAAATGGGGA GGGAGAAGTG CCATCAGTAC TGGCCTGCAG AGCGCTCAGC ACGGTACCAG 
TATTTTGTGG TGGACCCTAT GGCTGAGTAT AACATGCCTC AGTATATCCT GCGTGAGTTC 
AAAGTGACAG ATGCACGGGA TGGCCAATCT CGTACAATTA GACAATTCCA GTTCACTGAT 
TGGCCTGAAC AAGGAGTGCC AAAAACAGGT GAAGGCTTTA TTGACTTCAT TGGGCAGGTG 
CACAAAACCA AGGAGCAGTT TGGGCAGGAT GGGCCAATCA CCGTTCACTG CAGCGCTGGC 
GTGGGCCGCA CTGGAGTATT CATCACTCTC AGTATTGTAC TGGAGCGTAT GAGGTACGAG 
GGTGTGGTTG ACATGTTTCA AACAGTGAAG ACTTTAAGAA CACAGCGGCC AGCAATGGTA 
CAAACAGAGG ACCAGTATCA ACTGTGCTAT AGAGCCGCAC TGGAGTATCT GGGCAGCTTT 
GACCACTATG CAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_031900929.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756789.1 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X8, mRNA.

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CloneID	OXa57134
Clone ID Related Accession (Same CDS sequence)	XM_031900929.1
Accession Version	XM_031900929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTGAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACACT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGACATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCGAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTGACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTTCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA AGATGCTGTT ATCACTGGCC TCTCTCCTGA GACCACTTAT 
TCCATCACTG TGGCCGCCTA CACTACTAAG GGAGATGGTG CTAGAAGCAA ACCCAAGGTC 
GTTAGCACCA CAGGAGCTGT GCCTGGTAAA CCCACTATGA TGATCAGCAC CACAGCACTG 
AACACTGCTC TGATCCAGTG GCATCCTCCA AGAGAGATTG TGGGAGAGTT GTTAGGATAT 
CGTTTGCAGT ACAAGCGAGT GGATGAAGAC AGGTTTATTA CAATTGATTT TAGCAAGAAT 
GAACATCACC ACACAGTGAC TGGGCTACAT AAAGGTGCAA GCTATATATT CAAGCTTTCT 
GCAAAAAACC GAGCTGGAGC AGGGGAGGAA TATGAAAAGG AAATTTCCAC AGCTGAAGAT 
TTTCCTAGTG GGTTTCCCCA AAATCTGCAA GTAGTTGGAC TTACAACCTC TACTACAGAA 
GTGTCCTGGG AACCACCAGT GTTGTCTGAG CGCAATGGAA AGATCACCAA CTACACAGTG 
GTGTATCGGG ACATAAATAG CCAGCAGGAT CTCACCAATG TCACTCGCGA CACACACATA 
ACATTGGTCA ATCTCAAGCC AGACACCACA TATGACATCA AGGTCAGGGC AAGAACAAAC 
AAAGGCGCTG GCCCTCTAAG TCCTAGCATT CAATCACGTA CAATGCCTGT TGACCAAGTG 
TTTGCTAAGA ATTTCAGAGT AAACGCCGTG ACAAAAACCT CAGTCTTGCT AAGCTGGGAG 
CTGCCTGATT CTTACAAATC AGCTGTGCCT TTCAAGATCT TTTACAATAA TCAACATGTA 
GAAGTTGATG GACACTCTAT GCGCAAACTG ATCAGTAATC TCAAGCCAGA TACAGAATAC 
TCCTTTGTGC TGATGAACAG GGGGAGCAGT GCAGGTGGAC TGCAGCACCG CGTGTCCATT 
CGCACAGCAC CAGATGTTTT AAAAAGCAAG CCTTTTGCTG CCAACAAATA CATGGAGGAG 
GGCAGGTTCA CCTTGCTGTT GCCTCAGGTT CAGACTACTT CCCCAGTAAG GTGGTATTAT 
ATTGTGGTTG TCCCACTGGA CAGTTCCAGC GGGGGTCTGA ATTCTCGCTG GCAGAGCCCT 
GAGGAAATGG AGCTAGAGCA GATACTTGAA GCAATAAACC AGGGATCCCG TCGTCATAGA 
AGACAGGCAA GCAAGAATAA GCCATACATT GCAGCCCGAG TAAACACACT TCCAGAATCC 
TTTATTTTGG GTGACAATAA AGAATATAAT GGGTTCATCA ACAAGCCACT GAATCAGAAT 
TTACGCTACA CCTGCTTTGT GCTAGCTGCA CTAGAAGATG GGGACACTAA AAAGTATGCA 
GCCAGCCCAT ACTCAGATGA TATTGTGGTG GAGGTGATCT CACAGGCAAA ACAACAGGAA 
GAGCCAGAAA TGCTTTGGGT CATGGGTCCT GTTCTGGCTG TAATTCTTAT AATAATTATA 
GTTATTGCCA TTCTACTTTT CAAAAGTAAA CAGGAAAGGA AAAGGACCAA CTCTCCTTCA 
GGCAAAGATG AACAGTCAGT AGGACTTAAA GACTGTCTCC TCGCTCACTC CTCAGATCCA 
GTTGAAATGA GGCGTCTAAA CTACCAAACT CCAGGTTCCA GTGTCCCCAG TTACCCGAAT 
ATCTCAAGTA TGAGAGACCA CCCTCCTATT CCAATCTCTG ACCTGGCAGA CAACATTGAA 
AGACTGAAGG CTAATGATGG ACTCAAGTTC TCTCAGGAAT ATGAGTCTAT TGATCCAGGT 
CAGCAATTCA CGTGGGAAAA TTCAAATCTA GAAGTAAACA AACCAAAGAA TCGCTATGCA 
AATGTCATTG CATATGACCA TTCAAGGGTC ATTCTTACCT CAGTGGATGG AGTTCCCGGA 
AGTGATTACA TTAATGCCAA TTACATAGAT GGGTACAGGA AACAGAATGC CTATATAGCT 
ACGCAGGGCC CACTGCCAGA GACTATCACT GATTTCTGGA GAATGGTGTG GGAGCAAAGA 
ACCGCCACTA TTGTCATGAT GACCCGGCTA GAGGAAAAAT CAAGGGTGAA ATGTGACCAG 
TACTGGCCCG GTCGTGGCTC AGAGACCTAT GGTATGATCC AAGTCACATT AATGGACACA 
GTAGAGCTTG CCACCTACAC AGTTAGGACT TTCACACTTT ATAAGAATGG CTCCAGTGAG 
AAGCGGGATG TCAGACAGTT CCAATTTATG GCCTGGCCTG ATCACGGGGT CCCTGAATAT 
CCTACTCCTA TTCTTGGATT CCTTCGCAGA GTGAAAGGCT GTAATCCACC TGATGCTGGT 
CCTATGGTTG TTCATTGCAG TGCTGGCGTG GGCAGAACAG GATGCTTTAT TGTTATTGAT 
GCCATGCTAG AACGGGTGAA ACTGGAGAAA ACTGTGGATA TTTATGGTCA TGTGACCTGC 
ATGCGCTCCC AACGAAATTA TATGGTGCAA ACAGAGGACC AGTACATCTT TATCCATGAG 
GCTTTGCTTG AAGCAGTAAT GTGCGGGATC ACTGAAGTGC CAGCACGCAG TCTGTATGCA 
CATATCCAGA AACTGTCTCA GGTCCCTCCG GGTGAGAGTG TTACCTCCAT GGAACTAGAG 
TTCAAGCTTT TGGCCAACTC TAAAGCCCAC ACCTCACGAT TTATCAGTGC CAACCTCCCA 
TGCAACAAGT TCAAAAACAG ACTGGTAAAC ATCATGCCAT ATGAACTGAG TCGTGTCTCC 
CTGCAGCCCA TTCGTGGTGT TGAGGGATCA GATTATATCA ATGCAAGTTT CATTGATGGC 
TACAGGCAGC AGAAAGGCTA CATTGCTACT CAGGGTCCAT TGGCCGAGAC CACTGAGGAT 
TTCTGGAGAA TGCTGTGGGA ACATAACTCC ACCATTGTGG TGATGCTGAC AAAGTTGCGA 
GAAATGGGGA GGGAGAAGTG CCATCAGTAC TGGCCTGCAG AGCGCTCAGC ACGGTACCAG 
TATTTTGTGG TGGACCCTAT GGCTGAGTAT AACATGCCTC AGTATATCCT GCGTGAGTTC 
AAAGTGACAG ATGCACGGGA TGGCCAATCT CGTACAATTA GACAATTCCA GTTCACTGAT 
TGGCCTGAAC AAGGAGTGCC AAAAACAGGT GAAGGCTTTA TTGACTTCAT TGGGCAGGTG 
CACAAAACCA AGGAGCAGTT TGGGCAGGAT GGGCCAATCA CCGTTCACTG CAGCGCTGGC 
GTGGGCCGCA CTGGAGTATT CATCACTCTC AGTATTGTAC TGGAGCGTAT GAGGTACGAG 
GGTGTGGTTG ACATGTTTCA AACAGTGAAG ACTTTAAGAA CACAGCGGCC AGCAATGGTA 
CAAACAGAGG ACCAGTATCA ACTGTGCTAT AGAGCCGCAC TGGAGTATCT GGGCAGCTTT 
GACCACTATG CAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756789.1
CDS	636..6353
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKSKQERKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGSSVPSYPNISSMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_031900929.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900929.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTGAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACACT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGACATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCGAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTGACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTTCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA AGATGCTGTT ATCACTGGCC TCTCTCCTGA GACCACTTAT 
TCCATCACTG TGGCCGCCTA CACTACTAAG GGAGATGGTG CTAGAAGCAA ACCCAAGGTC 
GTTAGCACCA CAGGAGCTGT GCCTGGTAAA CCCACTATGA TGATCAGCAC CACAGCACTG 
AACACTGCTC TGATCCAGTG GCATCCTCCA AGAGAGATTG TGGGAGAGTT GTTAGGATAT 
CGTTTGCAGT ACAAGCGAGT GGATGAAGAC AGGTTTATTA CAATTGATTT TAGCAAGAAT 
GAACATCACC ACACAGTGAC TGGGCTACAT AAAGGTGCAA GCTATATATT CAAGCTTTCT 
GCAAAAAACC GAGCTGGAGC AGGGGAGGAA TATGAAAAGG AAATTTCCAC AGCTGAAGAT 
TTTCCTAGTG GGTTTCCCCA AAATCTGCAA GTAGTTGGAC TTACAACCTC TACTACAGAA 
GTGTCCTGGG AACCACCAGT GTTGTCTGAG CGCAATGGAA AGATCACCAA CTACACAGTG 
GTGTATCGGG ACATAAATAG CCAGCAGGAT CTCACCAATG TCACTCGCGA CACACACATA 
ACATTGGTCA ATCTCAAGCC AGACACCACA TATGACATCA AGGTCAGGGC AAGAACAAAC 
AAAGGCGCTG GCCCTCTAAG TCCTAGCATT CAATCACGTA CAATGCCTGT TGACCAAGTG 
TTTGCTAAGA ATTTCAGAGT AAACGCCGTG ACAAAAACCT CAGTCTTGCT AAGCTGGGAG 
CTGCCTGATT CTTACAAATC AGCTGTGCCT TTCAAGATCT TTTACAATAA TCAACATGTA 
GAAGTTGATG GACACTCTAT GCGCAAACTG ATCAGTAATC TCAAGCCAGA TACAGAATAC 
TCCTTTGTGC TGATGAACAG GGGGAGCAGT GCAGGTGGAC TGCAGCACCG CGTGTCCATT 
CGCACAGCAC CAGATGTTTT AAAAAGCAAG CCTTTTGCTG CCAACAAATA CATGGAGGAG 
GGCAGGTTCA CCTTGCTGTT GCCTCAGGTT CAGACTACTT CCCCAGTAAG GTGGTATTAT 
ATTGTGGTTG TCCCACTGGA CAGTTCCAGC GGGGGTCTGA ATTCTCGCTG GCAGAGCCCT 
GAGGAAATGG AGCTAGAGCA GATACTTGAA GCAATAAACC AGGGATCCCG TCGTCATAGA 
AGACAGGCAA GCAAGAATAA GCCATACATT GCAGCCCGAG TAAACACACT TCCAGAATCC 
TTTATTTTGG GTGACAATAA AGAATATAAT GGGTTCATCA ACAAGCCACT GAATCAGAAT 
TTACGCTACA CCTGCTTTGT GCTAGCTGCA CTAGAAGATG GGGACACTAA AAAGTATGCA 
GCCAGCCCAT ACTCAGATGA TATTGTGGTG GAGGTGATCT CACAGGCAAA ACAACAGGAA 
GAGCCAGAAA TGCTTTGGGT CATGGGTCCT GTTCTGGCTG TAATTCTTAT AATAATTATA 
GTTATTGCCA TTCTACTTTT CAAAAGTAAA CAGGAAAGGA AAAGGACCAA CTCTCCTTCA 
GGCAAAGATG AACAGTCAGT AGGACTTAAA GACTGTCTCC TCGCTCACTC CTCAGATCCA 
GTTGAAATGA GGCGTCTAAA CTACCAAACT CCAGGTTCCA GTGTCCCCAG TTACCCGAAT 
ATCTCAAGTA TGAGAGACCA CCCTCCTATT CCAATCTCTG ACCTGGCAGA CAACATTGAA 
AGACTGAAGG CTAATGATGG ACTCAAGTTC TCTCAGGAAT ATGAGTCTAT TGATCCAGGT 
CAGCAATTCA CGTGGGAAAA TTCAAATCTA GAAGTAAACA AACCAAAGAA TCGCTATGCA 
AATGTCATTG CATATGACCA TTCAAGGGTC ATTCTTACCT CAGTGGATGG AGTTCCCGGA 
AGTGATTACA TTAATGCCAA TTACATAGAT GGGTACAGGA AACAGAATGC CTATATAGCT 
ACGCAGGGCC CACTGCCAGA GACTATCACT GATTTCTGGA GAATGGTGTG GGAGCAAAGA 
ACCGCCACTA TTGTCATGAT GACCCGGCTA GAGGAAAAAT CAAGGGTGAA ATGTGACCAG 
TACTGGCCCG GTCGTGGCTC AGAGACCTAT GGTATGATCC AAGTCACATT AATGGACACA 
GTAGAGCTTG CCACCTACAC AGTTAGGACT TTCACACTTT ATAAGAATGG CTCCAGTGAG 
AAGCGGGATG TCAGACAGTT CCAATTTATG GCCTGGCCTG ATCACGGGGT CCCTGAATAT 
CCTACTCCTA TTCTTGGATT CCTTCGCAGA GTGAAAGGCT GTAATCCACC TGATGCTGGT 
CCTATGGTTG TTCATTGCAG TGCTGGCGTG GGCAGAACAG GATGCTTTAT TGTTATTGAT 
GCCATGCTAG AACGGGTGAA ACTGGAGAAA ACTGTGGATA TTTATGGTCA TGTGACCTGC 
ATGCGCTCCC AACGAAATTA TATGGTGCAA ACAGAGGACC AGTACATCTT TATCCATGAG 
GCTTTGCTTG AAGCAGTAAT GTGCGGGATC ACTGAAGTGC CAGCACGCAG TCTGTATGCA 
CATATCCAGA AACTGTCTCA GGTCCCTCCG GGTGAGAGTG TTACCTCCAT GGAACTAGAG 
TTCAAGCTTT TGGCCAACTC TAAAGCCCAC ACCTCACGAT TTATCAGTGC CAACCTCCCA 
TGCAACAAGT TCAAAAACAG ACTGGTAAAC ATCATGCCAT ATGAACTGAG TCGTGTCTCC 
CTGCAGCCCA TTCGTGGTGT TGAGGGATCA GATTATATCA ATGCAAGTTT CATTGATGGC 
TACAGGCAGC AGAAAGGCTA CATTGCTACT CAGGGTCCAT TGGCCGAGAC CACTGAGGAT 
TTCTGGAGAA TGCTGTGGGA ACATAACTCC ACCATTGTGG TGATGCTGAC AAAGTTGCGA 
GAAATGGGGA GGGAGAAGTG CCATCAGTAC TGGCCTGCAG AGCGCTCAGC ACGGTACCAG 
TATTTTGTGG TGGACCCTAT GGCTGAGTAT AACATGCCTC AGTATATCCT GCGTGAGTTC 
AAAGTGACAG ATGCACGGGA TGGCCAATCT CGTACAATTA GACAATTCCA GTTCACTGAT 
TGGCCTGAAC AAGGAGTGCC AAAAACAGGT GAAGGCTTTA TTGACTTCAT TGGGCAGGTG 
CACAAAACCA AGGAGCAGTT TGGGCAGGAT GGGCCAATCA CCGTTCACTG CAGCGCTGGC 
GTGGGCCGCA CTGGAGTATT CATCACTCTC AGTATTGTAC TGGAGCGTAT GAGGTACGAG 
GGTGTGGTTG ACATGTTTCA AACAGTGAAG ACTTTAAGAA CACAGCGGCC AGCAATGGTA 
CAAACAGAGG ACCAGTATCA ACTGTGCTAT AGAGCCGCAC TGGAGTATCT GGGCAGCTTT 
GACCACTATG CAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_004913961.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914018.2 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X9, mRNA.

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CloneID	OXa57135
Clone ID Related Accession (Same CDS sequence)	XM_004913961.2
Accession Version	XM_004913961.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913961.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTGAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACACT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGACATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCGAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTGACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTTCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA ATGGAGACCT GAGGATGTGG GAGATTACGA TGCTGTTATC 
ACTGGCCTCT CTCCTGAGAC CACTTATTCC ATCACTGTGG CCGCCTACAC TACTAAGGGA 
GATGGTGCTA GAAGCAAACC CAAGGTCGTT AGCACCACAG GAGCTGTGCC TGGTAAACCC 
ACTATGATGA TCAGCACCAC AGCACTGAAC ACTGCTCTGA TCCAGTGGCA TCCTCCAAGA 
GAGATTGTGG GAGAGTTGTT AGGATATCGT TTGCAGTACA AGCGAGTGGA TGAAGACAGG 
TTTATTACAA TTGATTTTAG CAAGAATGAA CATCACCACA CAGTGACTGG GCTACATAAA 
GGTGCAAGCT ATATATTCAA GCTTTCTGCA AAAAACCGAG CTGGAGCAGG GGAGGAATAT 
GAAAAGGAAA TTTCCACAGC TGAAGATTTT CCTAGTGGGT TTCCCCAAAA TCTGCAAGTA 
GTTGGACTTA CAACCTCTAC TACAGAAGTG TCCTGGGAAC CACCAGTGTT GTCTGAGCGC 
AATGGAAAGA TCACCAACTA CACAGTGGTG TATCGGGACA TAAATAGCCA GCAGGATCTC 
ACCAATGTCA CTCGCGACAC ACACATAACA TTGGTCAATC TCAAGCCAGA CACCACATAT 
GACATCAAGG TCAGGGCAAG AACAAACAAA GGCGCTGGCC CTCTAAGTCC TAGCATTCAA 
TCACGTACAA TGCCTGTTGA CCAAGTGTTT GCTAAGAATT TCAGAGTAAA CGCCGTGACA 
AAAACCTCAG TCTTGCTAAG CTGGGAGCTG CCTGATTCTT ACAAATCAGC TGTGCCTTTC 
AAGATCTTTT ACAATAATCA ACATGTAGAA GTTGATGGAC ACTCTATGCG CAAACTGATC 
AGTAATCTCA AGCCAGATAC AGAATACTCC TTTGTGCTGA TGAACAGGGG GAGCAGTGCA 
GGTGGACTGC AGCACCGCGT GTCCATTCGC ACAGCACCAG ATGTTTTAAA AAGCAAGCCT 
TTTGCTGCCA ACAAATACAT GGAGGAGGGC AGGTTCACCT TGCTGTTGCC TCAGGTTCAG 
ACTACTTCCC CAGTAAGGTG GTATTATATT GTGGTTGTCC CACTGGACAG TTCCAGCGGG 
GGTCTGAATT CTCGCTGGCA GAGCCCTGAG GAAATGGAGC TAGAGCAGAT ACTTGAAGCA 
ATAAACCAGG GATCCCGTCG TCATAGAAGA CAGGCAAGCA AGAATAAGCC ATACATTGCA 
GCCCGAGTAA ACACACTTCC AGAATCCTTT ATTTTGGGTG ACAATAAAGA ATATAATGGG 
TTCATCAACA AGCCACTGAA TCAGAATTTA CGCTACACCT GCTTTGTGCT AGCTGCACTA 
GAAGATGGGG ACACTAAAAA GTATGCAGCC AGCCCATACT CAGATGATAT TGTGGTGGAG 
GTGATCTCAC AGGCAAAACA ACAGGAAGAG CCAGAAATGC TTTGGGTCAT GGGTCCTGTT 
CTGGCTGTAA TTCTTATAAT AATTATAGTT ATTGCCATTC TACTTTTCAA AAGGAAAAGG 
ACCAACTCTC CTTCAGGCAA AGATGAACAG TCAGTAGGAC TTAAAGACTG TCTCCTCGCT 
CACTCCTCAG ATCCAGTTGA AATGAGGCGT CTAAACTACC AAACTCCAGG TATGAGAGAC 
CACCCTCCTA TTCCAATCTC TGACCTGGCA GACAACATTG AAAGACTGAA GGCTAATGAT 
GGACTCAAGT TCTCTCAGGA ATATGAGTCT ATTGATCCAG GTCAGCAATT CACGTGGGAA 
AATTCAAATC TAGAAGTAAA CAAACCAAAG AATCGCTATG CAAATGTCAT TGCATATGAC 
CATTCAAGGG TCATTCTTAC CTCAGTGGAT GGAGTTCCCG GAAGTGATTA CATTAATGCC 
AATTACATAG ATGGGTACAG GAAACAGAAT GCCTATATAG CTACGCAGGG CCCACTGCCA 
GAGACTATCA CTGATTTCTG GAGAATGGTG TGGGAGCAAA GAACCGCCAC TATTGTCATG 
ATGACCCGGC TAGAGGAAAA ATCAAGGGTG AAATGTGACC AGTACTGGCC CGGTCGTGGC 
TCAGAGACCT ATGGTATGAT CCAAGTCACA TTAATGGACA CAGTAGAGCT TGCCACCTAC 
ACAGTTAGGA CTTTCACACT TTATAAGAAT GGCTCCAGTG AGAAGCGGGA TGTCAGACAG 
TTCCAATTTA TGGCCTGGCC TGATCACGGG GTCCCTGAAT ATCCTACTCC TATTCTTGGA 
TTCCTTCGCA GAGTGAAAGG CTGTAATCCA CCTGATGCTG GTCCTATGGT TGTTCATTGC 
AGTGCTGGCG TGGGCAGAAC AGGATGCTTT ATTGTTATTG ATGCCATGCT AGAACGGGTG 
AAACTGGAGA AAACTGTGGA TATTTATGGT CATGTGACCT GCATGCGCTC CCAACGAAAT 
TATATGGTGC AAACAGAGGA CCAGTACATC TTTATCCATG AGGCTTTGCT TGAAGCAGTA 
ATGTGCGGGA TCACTGAAGT GCCAGCACGC AGTCTGTATG CACATATCCA GAAACTGTCT 
CAGGTCCCTC CGGGTGAGAG TGTTACCTCC ATGGAACTAG AGTTCAAGCT TTTGGCCAAC 
TCTAAAGCCC ACACCTCACG ATTTATCAGT GCCAACCTCC CATGCAACAA GTTCAAAAAC 
AGACTGGTAA ACATCATGCC ATATGAACTG AGTCGTGTCT CCCTGCAGCC CATTCGTGGT 
GTTGAGGGAT CAGATTATAT CAATGCAAGT TTCATTGATG GCTACAGGCA GCAGAAAGGC 
TACATTGCTA CTCAGGGTCC ATTGGCCGAG ACCACTGAGG ATTTCTGGAG AATGCTGTGG 
GAACATAACT CCACCATTGT GGTGATGCTG ACAAAGTTGC GAGAAATGGG GAGGGAGAAG 
TGCCATCAGT ACTGGCCTGC AGAGCGCTCA GCACGGTACC AGTATTTTGT GGTGGACCCT 
ATGGCTGAGT ATAACATGCC TCAGTATATC CTGCGTGAGT TCAAAGTGAC AGATGCACGG 
GATGGCCAAT CTCGTACAAT TAGACAATTC CAGTTCACTG ATTGGCCTGA ACAAGGAGTG 
CCAAAAACAG GTGAAGGCTT TATTGACTTC ATTGGGCAGG TGCACAAAAC CAAGGAGCAG 
TTTGGGCAGG ATGGGCCAAT CACCGTTCAC TGCAGCGCTG GCGTGGGCCG CACTGGAGTA 
TTCATCACTC TCAGTATTGT ACTGGAGCGT ATGAGGTACG AGGGTGTGGT TGACATGTTT 
CAAACAGTGA AGACTTTAAG AACACAGCGG CCAGCAATGG TACAAACAGA GGACCAGTAT 
CAACTGTGCT ATAGAGCCGC ACTGGAGTAT CTGGGCAGCT TTGACCACTA TGCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914018.2
CDS	636..6335
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQWRPEDVGDYDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKRKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_004913961.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913961.2

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTGAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACACT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGACATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCGAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTGACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTTCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA ATGGAGACCT GAGGATGTGG GAGATTACGA TGCTGTTATC 
ACTGGCCTCT CTCCTGAGAC CACTTATTCC ATCACTGTGG CCGCCTACAC TACTAAGGGA 
GATGGTGCTA GAAGCAAACC CAAGGTCGTT AGCACCACAG GAGCTGTGCC TGGTAAACCC 
ACTATGATGA TCAGCACCAC AGCACTGAAC ACTGCTCTGA TCCAGTGGCA TCCTCCAAGA 
GAGATTGTGG GAGAGTTGTT AGGATATCGT TTGCAGTACA AGCGAGTGGA TGAAGACAGG 
TTTATTACAA TTGATTTTAG CAAGAATGAA CATCACCACA CAGTGACTGG GCTACATAAA 
GGTGCAAGCT ATATATTCAA GCTTTCTGCA AAAAACCGAG CTGGAGCAGG GGAGGAATAT 
GAAAAGGAAA TTTCCACAGC TGAAGATTTT CCTAGTGGGT TTCCCCAAAA TCTGCAAGTA 
GTTGGACTTA CAACCTCTAC TACAGAAGTG TCCTGGGAAC CACCAGTGTT GTCTGAGCGC 
AATGGAAAGA TCACCAACTA CACAGTGGTG TATCGGGACA TAAATAGCCA GCAGGATCTC 
ACCAATGTCA CTCGCGACAC ACACATAACA TTGGTCAATC TCAAGCCAGA CACCACATAT 
GACATCAAGG TCAGGGCAAG AACAAACAAA GGCGCTGGCC CTCTAAGTCC TAGCATTCAA 
TCACGTACAA TGCCTGTTGA CCAAGTGTTT GCTAAGAATT TCAGAGTAAA CGCCGTGACA 
AAAACCTCAG TCTTGCTAAG CTGGGAGCTG CCTGATTCTT ACAAATCAGC TGTGCCTTTC 
AAGATCTTTT ACAATAATCA ACATGTAGAA GTTGATGGAC ACTCTATGCG CAAACTGATC 
AGTAATCTCA AGCCAGATAC AGAATACTCC TTTGTGCTGA TGAACAGGGG GAGCAGTGCA 
GGTGGACTGC AGCACCGCGT GTCCATTCGC ACAGCACCAG ATGTTTTAAA AAGCAAGCCT 
TTTGCTGCCA ACAAATACAT GGAGGAGGGC AGGTTCACCT TGCTGTTGCC TCAGGTTCAG 
ACTACTTCCC CAGTAAGGTG GTATTATATT GTGGTTGTCC CACTGGACAG TTCCAGCGGG 
GGTCTGAATT CTCGCTGGCA GAGCCCTGAG GAAATGGAGC TAGAGCAGAT ACTTGAAGCA 
ATAAACCAGG GATCCCGTCG TCATAGAAGA CAGGCAAGCA AGAATAAGCC ATACATTGCA 
GCCCGAGTAA ACACACTTCC AGAATCCTTT ATTTTGGGTG ACAATAAAGA ATATAATGGG 
TTCATCAACA AGCCACTGAA TCAGAATTTA CGCTACACCT GCTTTGTGCT AGCTGCACTA 
GAAGATGGGG ACACTAAAAA GTATGCAGCC AGCCCATACT CAGATGATAT TGTGGTGGAG 
GTGATCTCAC AGGCAAAACA ACAGGAAGAG CCAGAAATGC TTTGGGTCAT GGGTCCTGTT 
CTGGCTGTAA TTCTTATAAT AATTATAGTT ATTGCCATTC TACTTTTCAA AAGGAAAAGG 
ACCAACTCTC CTTCAGGCAA AGATGAACAG TCAGTAGGAC TTAAAGACTG TCTCCTCGCT 
CACTCCTCAG ATCCAGTTGA AATGAGGCGT CTAAACTACC AAACTCCAGG TATGAGAGAC 
CACCCTCCTA TTCCAATCTC TGACCTGGCA GACAACATTG AAAGACTGAA GGCTAATGAT 
GGACTCAAGT TCTCTCAGGA ATATGAGTCT ATTGATCCAG GTCAGCAATT CACGTGGGAA 
AATTCAAATC TAGAAGTAAA CAAACCAAAG AATCGCTATG CAAATGTCAT TGCATATGAC 
CATTCAAGGG TCATTCTTAC CTCAGTGGAT GGAGTTCCCG GAAGTGATTA CATTAATGCC 
AATTACATAG ATGGGTACAG GAAACAGAAT GCCTATATAG CTACGCAGGG CCCACTGCCA 
GAGACTATCA CTGATTTCTG GAGAATGGTG TGGGAGCAAA GAACCGCCAC TATTGTCATG 
ATGACCCGGC TAGAGGAAAA ATCAAGGGTG AAATGTGACC AGTACTGGCC CGGTCGTGGC 
TCAGAGACCT ATGGTATGAT CCAAGTCACA TTAATGGACA CAGTAGAGCT TGCCACCTAC 
ACAGTTAGGA CTTTCACACT TTATAAGAAT GGCTCCAGTG AGAAGCGGGA TGTCAGACAG 
TTCCAATTTA TGGCCTGGCC TGATCACGGG GTCCCTGAAT ATCCTACTCC TATTCTTGGA 
TTCCTTCGCA GAGTGAAAGG CTGTAATCCA CCTGATGCTG GTCCTATGGT TGTTCATTGC 
AGTGCTGGCG TGGGCAGAAC AGGATGCTTT ATTGTTATTG ATGCCATGCT AGAACGGGTG 
AAACTGGAGA AAACTGTGGA TATTTATGGT CATGTGACCT GCATGCGCTC CCAACGAAAT 
TATATGGTGC AAACAGAGGA CCAGTACATC TTTATCCATG AGGCTTTGCT TGAAGCAGTA 
ATGTGCGGGA TCACTGAAGT GCCAGCACGC AGTCTGTATG CACATATCCA GAAACTGTCT 
CAGGTCCCTC CGGGTGAGAG TGTTACCTCC ATGGAACTAG AGTTCAAGCT TTTGGCCAAC 
TCTAAAGCCC ACACCTCACG ATTTATCAGT GCCAACCTCC CATGCAACAA GTTCAAAAAC 
AGACTGGTAA ACATCATGCC ATATGAACTG AGTCGTGTCT CCCTGCAGCC CATTCGTGGT 
GTTGAGGGAT CAGATTATAT CAATGCAAGT TTCATTGATG GCTACAGGCA GCAGAAAGGC 
TACATTGCTA CTCAGGGTCC ATTGGCCGAG ACCACTGAGG ATTTCTGGAG AATGCTGTGG 
GAACATAACT CCACCATTGT GGTGATGCTG ACAAAGTTGC GAGAAATGGG GAGGGAGAAG 
TGCCATCAGT ACTGGCCTGC AGAGCGCTCA GCACGGTACC AGTATTTTGT GGTGGACCCT 
ATGGCTGAGT ATAACATGCC TCAGTATATC CTGCGTGAGT TCAAAGTGAC AGATGCACGG 
GATGGCCAAT CTCGTACAAT TAGACAATTC CAGTTCACTG ATTGGCCTGA ACAAGGAGTG 
CCAAAAACAG GTGAAGGCTT TATTGACTTC ATTGGGCAGG TGCACAAAAC CAAGGAGCAG 
TTTGGGCAGG ATGGGCCAAT CACCGTTCAC TGCAGCGCTG GCGTGGGCCG CACTGGAGTA 
TTCATCACTC TCAGTATTGT ACTGGAGCGT ATGAGGTACG AGGGTGTGGT TGACATGTTT 
CAAACAGTGA AGACTTTAAG AACACAGCGG CCAGCAATGG TACAAACAGA GGACCAGTAT 
CAACTGTGCT ATAGAGCCGC ACTGGAGTAT CTGGGCAGCT TTGACCACTA TGCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_031900930.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756790.1 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X10, mRNA.

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CloneID	OXa57136
Clone ID Related Accession (Same CDS sequence)	XM_031900930.1
Accession Version	XM_031900930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAC ACCCTCAGCC CCGCCACAGG ATGTGGAGTG CTCGAGTCTT 
AGCTCCACTG CCATCCGGGT AAGTTGGGTG GCCCCTCCAG CTTCCAGCCG CAACGGGGCC 
CTGACCCGCT ACTCACTTGC CTACCAAGCC CTGGCTGGGG AGGACACATC CCGGCATGTT 
GTGGACAGCA TCTCACCAGA TATCTCCAGC TGGGAGATCA AGGATCTGGA GAAATGGACT 
GAGTACAAAG TATGGGTGCG AGCTCATACA GATGTAGGGG CTGGGCCTGA AAGCTCCCCC 
ATCATTGTGC GGACAGATGA GGATGTGCCG AGTGCACCAC CAAGAAAGGT TGAAGTAGAC 
TCTGTAAACT CCACATCTGT TCGTGTGACA TGGAAGTCTC CTCTACCAAC CAAGCAAAAT 
GGGCAAATTA GGGGGTATCA AGTCACTTAT GTTCGTCTAG AAGGTGGAGA GCCTCGGGGG 
AATCCCATCA TAAAAGATGT TATGCTGTCA GAGGCTCAAG ATGCTGTTAT CACTGGCCTC 
TCTCCTGAGA CCACTTATTC CATCACTGTG GCCGCCTACA CTACTAAGGG AGATGGTGCT 
AGAAGCAAAC CCAAGGTCGT TAGCACCACA GGAGCTGTGC CTGGTAAACC CACTATGATG 
ATCAGCACCA CAGCACTGAA CACTGCTCTG ATCCAGTGGC ATCCTCCAAG AGAGATTGTG 
GGAGAGTTGT TAGGATATCG TTTGCAGTAC AAGCGAGTGG ATGAAGACAG GTTTATTACA 
ATTGATTTTA GCAAGAATGA ACATCACCAC ACAGTGACTG GGCTACATAA AGGTGCAAGC 
TATATATTCA AGCTTTCTGC AAAAAACCGA GCTGGAGCAG GGGAGGAATA TGAAAAGGAA 
ATTTCCACAG CTGAAGATTT TCCTAGTGGG TTTCCCCAAA ATCTGCAAGT AGTTGGACTT 
ACAACCTCTA CTACAGAAGT GTCCTGGGAA CCACCAGTGT TGTCTGAGCG CAATGGAAAG 
ATCACCAACT ACACAGTGGT GTATCGGGAC ATAAATAGCC AGCAGGATCT CACCAATGTC 
ACTCGCGACA CACACATAAC ATTGGTCAAT CTCAAGCCAG ACACCACATA TGACATCAAG 
GTCAGGGCAA GAACAAACAA AGGCGCTGGC CCTCTAAGTC CTAGCATTCA ATCACGTACA 
ATGCCTGTTG ACCAAGTGTT TGCTAAGAAT TTCAGAGTAA ACGCCGTGAC AAAAACCTCA 
GTCTTGCTAA GCTGGGAGCT GCCTGATTCT TACAAATCAG CTGTGCCTTT CAAGATCTTT 
TACAATAATC AACATGTAGA AGTTGATGGA CACTCTATGC GCAAACTGAT CAGTAATCTC 
AAGCCAGATA CAGAATACTC CTTTGTGCTG ATGAACAGGG GGAGCAGTGC AGGTGGACTG 
CAGCACCGCG TGTCCATTCG CACAGCACCA GATGTTTTAA AAAGCAAGCC TTTTGCTGCC 
AACAAATACA TGGAGGAGGG CAGGTTCACC TTGCTGTTGC CTCAGGTTCA GACTACTTCC 
CCAGTAAGGT GGTATTATAT TGTGGTTGTC CCACTGGACA GTTCCAGCGG GGGTCTGAAT 
TCTCGCTGGC AGAGCCCTGA GGAAATGGAG CTAGAGCAGA TACTTGAAGC AATAAACCAG 
GGATCCCGTC GTCATAGAAG ACAGGCAAGC AAGAATAAGC CATACATTGC AGCCCGAGTA 
AACACACTTC CAGAATCCTT TATTTTGGGT GACAATAAAG AATATAATGG GTTCATCAAC 
AAGCCACTGA ATCAGAATTT ACGCTACACC TGCTTTGTGC TAGCTGCACT AGAAGATGGG 
GACACTAAAA AGTATGCAGC CAGCCCATAC TCAGATGATA TTGTGGTGGA GGTGATCTCA 
CAGGCAAAAC AACAGGAAGA GCCAGAAATG CTTTGGGTCA TGGGTCCTGT TCTGGCTGTA 
ATTCTTATAA TAATTATAGT TATTGCCATT CTACTTTTCA AAAGGAAAAG GACCAACTCT 
CCTTCAGGCA AAGATGAACA GTCAGTAGGA CTTAAAGACT GTCTCCTCGC TCACTCCTCA 
GATCCAGTTG AAATGAGGCG TCTAAACTAC CAAACTCCAG GTATGAGAGA CCACCCTCCT 
ATTCCAATCT CTGACCTGGC AGACAACATT GAAAGACTGA AGGCTAATGA TGGACTCAAG 
TTCTCTCAGG AATATGAGTC TATTGATCCA GGTCAGCAAT TCACGTGGGA AAATTCAAAT 
CTAGAAGTAA ACAAACCAAA GAATCGCTAT GCAAATGTCA TTGCATATGA CCATTCAAGG 
GTCATTCTTA CCTCAGTGGA TGGAGTTCCC GGAAGTGATT ACATTAATGC CAATTACATA 
GATGGGTACA GGAAACAGAA TGCCTATATA GCTACGCAGG GCCCACTGCC AGAGACTATC 
ACTGATTTCT GGAGAATGGT GTGGGAGCAA AGAACCGCCA CTATTGTCAT GATGACCCGG 
CTAGAGGAAA AATCAAGGGT GAAATGTGAC CAGTACTGGC CCGGTCGTGG CTCAGAGACC 
TATGGTATGA TCCAAGTCAC ATTAATGGAC ACAGTAGAGC TTGCCACCTA CACAGTTAGG 
ACTTTCACAC TTTATAAGAA TGGCTCCAGT GAGAAGCGGG ATGTCAGACA GTTCCAATTT 
ATGGCCTGGC CTGATCACGG GGTCCCTGAA TATCCTACTC CTATTCTTGG ATTCCTTCGC 
AGAGTGAAAG GCTGTAATCC ACCTGATGCT GGTCCTATGG TTGTTCATTG CAGTGCTGGC 
GTGGGCAGAA CAGGATGCTT TATTGTTATT GATGCCATGC TAGAACGGGT GAAACTGGAG 
AAAACTGTGG ATATTTATGG TCATGTGACC TGCATGCGCT CCCAACGAAA TTATATGGTG 
CAAACAGAGG ACCAGTACAT CTTTATCCAT GAGGCTTTGC TTGAAGCAGT AATGTGCGGG 
ATCACTGAAG TGCCAGCACG CAGTCTGTAT GCACATATCC AGAAACTGTC TCAGGTCCCT 
CCGGGTGAGA GTGTTACCTC CATGGAACTA GAGTTCAAGC TTTTGGCCAA CTCTAAAGCC 
CACACCTCAC GATTTATCAG TGCCAACCTC CCATGCAACA AGTTCAAAAA CAGACTGGTA 
AACATCATGC CATATGAACT GAGTCGTGTC TCCCTGCAGC CCATTCGTGG TGTTGAGGGA 
TCAGATTATA TCAATGCAAG TTTCATTGAT GGCTACAGGC AGCAGAAAGG CTACATTGCT 
ACTCAGGGTC CATTGGCCGA GACCACTGAG GATTTCTGGA GAATGCTGTG GGAACATAAC 
TCCACCATTG TGGTGATGCT GACAAAGTTG CGAGAAATGG GGAGGGAGAA GTGCCATCAG 
TACTGGCCTG CAGAGCGCTC AGCACGGTAC CAGTATTTTG TGGTGGACCC TATGGCTGAG 
TATAACATGC CTCAGTATAT CCTGCGTGAG TTCAAAGTGA CAGATGCACG GGATGGCCAA 
TCTCGTACAA TTAGACAATT CCAGTTCACT GATTGGCCTG AACAAGGAGT GCCAAAAACA 
GGTGAAGGCT TTATTGACTT CATTGGGCAG GTGCACAAAA CCAAGGAGCA GTTTGGGCAG 
GATGGGCCAA TCACCGTTCA CTGCAGCGCT GGCGTGGGCC GCACTGGAGT ATTCATCACT 
CTCAGTATTG TACTGGAGCG TATGAGGTAC GAGGGTGTGG TTGACATGTT TCAAACAGTG 
AAGACTTTAA GAACACAGCG GCCAGCAATG GTACAAACAG AGGACCAGTA TCAACTGTGC 
TATAGAGCCG CACTGGAGTA TCTGGGCAGC TTTGACCACT ATGCAACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756790.1
CDS	636..6326
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKRKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_031900930.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900930.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAC ACCCTCAGCC CCGCCACAGG ATGTGGAGTG CTCGAGTCTT 
AGCTCCACTG CCATCCGGGT AAGTTGGGTG GCCCCTCCAG CTTCCAGCCG CAACGGGGCC 
CTGACCCGCT ACTCACTTGC CTACCAAGCC CTGGCTGGGG AGGACACATC CCGGCATGTT 
GTGGACAGCA TCTCACCAGA TATCTCCAGC TGGGAGATCA AGGATCTGGA GAAATGGACT 
GAGTACAAAG TATGGGTGCG AGCTCATACA GATGTAGGGG CTGGGCCTGA AAGCTCCCCC 
ATCATTGTGC GGACAGATGA GGATGTGCCG AGTGCACCAC CAAGAAAGGT TGAAGTAGAC 
TCTGTAAACT CCACATCTGT TCGTGTGACA TGGAAGTCTC CTCTACCAAC CAAGCAAAAT 
GGGCAAATTA GGGGGTATCA AGTCACTTAT GTTCGTCTAG AAGGTGGAGA GCCTCGGGGG 
AATCCCATCA TAAAAGATGT TATGCTGTCA GAGGCTCAAG ATGCTGTTAT CACTGGCCTC 
TCTCCTGAGA CCACTTATTC CATCACTGTG GCCGCCTACA CTACTAAGGG AGATGGTGCT 
AGAAGCAAAC CCAAGGTCGT TAGCACCACA GGAGCTGTGC CTGGTAAACC CACTATGATG 
ATCAGCACCA CAGCACTGAA CACTGCTCTG ATCCAGTGGC ATCCTCCAAG AGAGATTGTG 
GGAGAGTTGT TAGGATATCG TTTGCAGTAC AAGCGAGTGG ATGAAGACAG GTTTATTACA 
ATTGATTTTA GCAAGAATGA ACATCACCAC ACAGTGACTG GGCTACATAA AGGTGCAAGC 
TATATATTCA AGCTTTCTGC AAAAAACCGA GCTGGAGCAG GGGAGGAATA TGAAAAGGAA 
ATTTCCACAG CTGAAGATTT TCCTAGTGGG TTTCCCCAAA ATCTGCAAGT AGTTGGACTT 
ACAACCTCTA CTACAGAAGT GTCCTGGGAA CCACCAGTGT TGTCTGAGCG CAATGGAAAG 
ATCACCAACT ACACAGTGGT GTATCGGGAC ATAAATAGCC AGCAGGATCT CACCAATGTC 
ACTCGCGACA CACACATAAC ATTGGTCAAT CTCAAGCCAG ACACCACATA TGACATCAAG 
GTCAGGGCAA GAACAAACAA AGGCGCTGGC CCTCTAAGTC CTAGCATTCA ATCACGTACA 
ATGCCTGTTG ACCAAGTGTT TGCTAAGAAT TTCAGAGTAA ACGCCGTGAC AAAAACCTCA 
GTCTTGCTAA GCTGGGAGCT GCCTGATTCT TACAAATCAG CTGTGCCTTT CAAGATCTTT 
TACAATAATC AACATGTAGA AGTTGATGGA CACTCTATGC GCAAACTGAT CAGTAATCTC 
AAGCCAGATA CAGAATACTC CTTTGTGCTG ATGAACAGGG GGAGCAGTGC AGGTGGACTG 
CAGCACCGCG TGTCCATTCG CACAGCACCA GATGTTTTAA AAAGCAAGCC TTTTGCTGCC 
AACAAATACA TGGAGGAGGG CAGGTTCACC TTGCTGTTGC CTCAGGTTCA GACTACTTCC 
CCAGTAAGGT GGTATTATAT TGTGGTTGTC CCACTGGACA GTTCCAGCGG GGGTCTGAAT 
TCTCGCTGGC AGAGCCCTGA GGAAATGGAG CTAGAGCAGA TACTTGAAGC AATAAACCAG 
GGATCCCGTC GTCATAGAAG ACAGGCAAGC AAGAATAAGC CATACATTGC AGCCCGAGTA 
AACACACTTC CAGAATCCTT TATTTTGGGT GACAATAAAG AATATAATGG GTTCATCAAC 
AAGCCACTGA ATCAGAATTT ACGCTACACC TGCTTTGTGC TAGCTGCACT AGAAGATGGG 
GACACTAAAA AGTATGCAGC CAGCCCATAC TCAGATGATA TTGTGGTGGA GGTGATCTCA 
CAGGCAAAAC AACAGGAAGA GCCAGAAATG CTTTGGGTCA TGGGTCCTGT TCTGGCTGTA 
ATTCTTATAA TAATTATAGT TATTGCCATT CTACTTTTCA AAAGGAAAAG GACCAACTCT 
CCTTCAGGCA AAGATGAACA GTCAGTAGGA CTTAAAGACT GTCTCCTCGC TCACTCCTCA 
GATCCAGTTG AAATGAGGCG TCTAAACTAC CAAACTCCAG GTATGAGAGA CCACCCTCCT 
ATTCCAATCT CTGACCTGGC AGACAACATT GAAAGACTGA AGGCTAATGA TGGACTCAAG 
TTCTCTCAGG AATATGAGTC TATTGATCCA GGTCAGCAAT TCACGTGGGA AAATTCAAAT 
CTAGAAGTAA ACAAACCAAA GAATCGCTAT GCAAATGTCA TTGCATATGA CCATTCAAGG 
GTCATTCTTA CCTCAGTGGA TGGAGTTCCC GGAAGTGATT ACATTAATGC CAATTACATA 
GATGGGTACA GGAAACAGAA TGCCTATATA GCTACGCAGG GCCCACTGCC AGAGACTATC 
ACTGATTTCT GGAGAATGGT GTGGGAGCAA AGAACCGCCA CTATTGTCAT GATGACCCGG 
CTAGAGGAAA AATCAAGGGT GAAATGTGAC CAGTACTGGC CCGGTCGTGG CTCAGAGACC 
TATGGTATGA TCCAAGTCAC ATTAATGGAC ACAGTAGAGC TTGCCACCTA CACAGTTAGG 
ACTTTCACAC TTTATAAGAA TGGCTCCAGT GAGAAGCGGG ATGTCAGACA GTTCCAATTT 
ATGGCCTGGC CTGATCACGG GGTCCCTGAA TATCCTACTC CTATTCTTGG ATTCCTTCGC 
AGAGTGAAAG GCTGTAATCC ACCTGATGCT GGTCCTATGG TTGTTCATTG CAGTGCTGGC 
GTGGGCAGAA CAGGATGCTT TATTGTTATT GATGCCATGC TAGAACGGGT GAAACTGGAG 
AAAACTGTGG ATATTTATGG TCATGTGACC TGCATGCGCT CCCAACGAAA TTATATGGTG 
CAAACAGAGG ACCAGTACAT CTTTATCCAT GAGGCTTTGC TTGAAGCAGT AATGTGCGGG 
ATCACTGAAG TGCCAGCACG CAGTCTGTAT GCACATATCC AGAAACTGTC TCAGGTCCCT 
CCGGGTGAGA GTGTTACCTC CATGGAACTA GAGTTCAAGC TTTTGGCCAA CTCTAAAGCC 
CACACCTCAC GATTTATCAG TGCCAACCTC CCATGCAACA AGTTCAAAAA CAGACTGGTA 
AACATCATGC CATATGAACT GAGTCGTGTC TCCCTGCAGC CCATTCGTGG TGTTGAGGGA 
TCAGATTATA TCAATGCAAG TTTCATTGAT GGCTACAGGC AGCAGAAAGG CTACATTGCT 
ACTCAGGGTC CATTGGCCGA GACCACTGAG GATTTCTGGA GAATGCTGTG GGAACATAAC 
TCCACCATTG TGGTGATGCT GACAAAGTTG CGAGAAATGG GGAGGGAGAA GTGCCATCAG 
TACTGGCCTG CAGAGCGCTC AGCACGGTAC CAGTATTTTG TGGTGGACCC TATGGCTGAG 
TATAACATGC CTCAGTATAT CCTGCGTGAG TTCAAAGTGA CAGATGCACG GGATGGCCAA 
TCTCGTACAA TTAGACAATT CCAGTTCACT GATTGGCCTG AACAAGGAGT GCCAAAAACA 
GGTGAAGGCT TTATTGACTT CATTGGGCAG GTGCACAAAA CCAAGGAGCA GTTTGGGCAG 
GATGGGCCAA TCACCGTTCA CTGCAGCGCT GGCGTGGGCC GCACTGGAGT ATTCATCACT 
CTCAGTATTG TACTGGAGCG TATGAGGTAC GAGGGTGTGG TTGACATGTT TCAAACAGTG 
AAGACTTTAA GAACACAGCG GCCAGCAATG GTACAAACAG AGGACCAGTA TCAACTGTGC 
TATAGAGCCG CACTGGAGTA TCTGGGCAGC TTTGACCACT ATGCAACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_004913963.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914020.2 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X11, mRNA.

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CloneID	OXa57137
Clone ID Related Accession (Same CDS sequence)	XM_004913963.2
Accession Version	XM_004913963.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913963.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTGAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACACT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGACATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCGAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTGACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTTCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA AGATGCTGTT ATCACTGGCC TCTCTCCTGA GACCACTTAT 
TCCATCACTG TGGCCGCCTA CACTACTAAG GGAGATGGTG CTAGAAGCAA ACCCAAGGTC 
GTTAGCACCA CAGGAGCTGT GCCTGGTAAA CCCACTATGA TGATCAGCAC CACAGCACTG 
AACACTGCTC TGATCCAGTG GCATCCTCCA AGAGAGATTG TGGGAGAGTT GTTAGGATAT 
CGTTTGCAGT ACAAGCGAGT GGATGAAGAC AGGTTTATTA CAATTGATTT TAGCAAGAAT 
GAACATCACC ACACAGTGAC TGGGCTACAT AAAGGTGCAA GCTATATATT CAAGCTTTCT 
GCAAAAAACC GAGCTGGAGC AGGGGAGGAA TATGAAAAGG AAATTTCCAC AGCTGAAGAT 
TTTCCTAGTG GGTTTCCCCA AAATCTGCAA GTAGTTGGAC TTACAACCTC TACTACAGAA 
GTGTCCTGGG AACCACCAGT GTTGTCTGAG CGCAATGGAA AGATCACCAA CTACACAGTG 
GTGTATCGGG ACATAAATAG CCAGCAGGAT CTCACCAATG TCACTCGCGA CACACACATA 
ACATTGGTCA ATCTCAAGCC AGACACCACA TATGACATCA AGGTCAGGGC AAGAACAAAC 
AAAGGCGCTG GCCCTCTAAG TCCTAGCATT CAATCACGTA CAATGCCTGT TGACCAAGTG 
TTTGCTAAGA ATTTCAGAGT AAACGCCGTG ACAAAAACCT CAGTCTTGCT AAGCTGGGAG 
CTGCCTGATT CTTACAAATC AGCTGTGCCT TTCAAGATCT TTTACAATAA TCAACATGTA 
GAAGTTGATG GACACTCTAT GCGCAAACTG ATCAGTAATC TCAAGCCAGA TACAGAATAC 
TCCTTTGTGC TGATGAACAG GGGGAGCAGT GCAGGTGGAC TGCAGCACCG CGTGTCCATT 
CGCACAGCAC CAGATGTTTT AAAAAGCAAG CCTTTTGCTG CCAACAAATA CATGGAGGAG 
GGCAGGTTCA CCTTGCTGTT GCCTCAGGTT CAGACTACTT CCCCAGTAAG GTGGTATTAT 
ATTGTGGTTG TCCCACTGGA CAGTTCCAGC GGGGGTCTGA ATTCTCGCTG GCAGAGCCCT 
GAGGAAATGG AGCTAGAGCA GATACTTGAA GCAATAAACC AGGGATCCCG TCGTCATAGA 
AGACAGGCAA GCAAGAATAA GCCATACATT GCAGCCCGAG TAAACACACT TCCAGAATCC 
TTTATTTTGG GTGACAATAA AGAATATAAT GGGTTCATCA ACAAGCCACT GAATCAGAAT 
TTACGCTACA CCTGCTTTGT GCTAGCTGCA CTAGAAGATG GGGACACTAA AAAGTATGCA 
GCCAGCCCAT ACTCAGATGA TATTGTGGTG GAGGTGATCT CACAGGCAAA ACAACAGGAA 
GAGCCAGAAA TGCTTTGGGT CATGGGTCCT GTTCTGGCTG TAATTCTTAT AATAATTATA 
GTTATTGCCA TTCTACTTTT CAAAAGGAAA AGGACCAACT CTCCTTCAGG CAAAGATGAA 
CAGTCAGTAG GACTTAAAGA CTGTCTCCTC GCTCACTCCT CAGATCCAGT TGAAATGAGG 
CGTCTAAACT ACCAAACTCC AGGTATGAGA GACCACCCTC CTATTCCAAT CTCTGACCTG 
GCAGACAACA TTGAAAGACT GAAGGCTAAT GATGGACTCA AGTTCTCTCA GGAATATGAG 
TCTATTGATC CAGGTCAGCA ATTCACGTGG GAAAATTCAA ATCTAGAAGT AAACAAACCA 
AAGAATCGCT ATGCAAATGT CATTGCATAT GACCATTCAA GGGTCATTCT TACCTCAGTG 
GATGGAGTTC CCGGAAGTGA TTACATTAAT GCCAATTACA TAGATGGGTA CAGGAAACAG 
AATGCCTATA TAGCTACGCA GGGCCCACTG CCAGAGACTA TCACTGATTT CTGGAGAATG 
GTGTGGGAGC AAAGAACCGC CACTATTGTC ATGATGACCC GGCTAGAGGA AAAATCAAGG 
GTGAAATGTG ACCAGTACTG GCCCGGTCGT GGCTCAGAGA CCTATGGTAT GATCCAAGTC 
ACATTAATGG ACACAGTAGA GCTTGCCACC TACACAGTTA GGACTTTCAC ACTTTATAAG 
AATGGCTCCA GTGAGAAGCG GGATGTCAGA CAGTTCCAAT TTATGGCCTG GCCTGATCAC 
GGGGTCCCTG AATATCCTAC TCCTATTCTT GGATTCCTTC GCAGAGTGAA AGGCTGTAAT 
CCACCTGATG CTGGTCCTAT GGTTGTTCAT TGCAGTGCTG GCGTGGGCAG AACAGGATGC 
TTTATTGTTA TTGATGCCAT GCTAGAACGG GTGAAACTGG AGAAAACTGT GGATATTTAT 
GGTCATGTGA CCTGCATGCG CTCCCAACGA AATTATATGG TGCAAACAGA GGACCAGTAC 
ATCTTTATCC ATGAGGCTTT GCTTGAAGCA GTAATGTGCG GGATCACTGA AGTGCCAGCA 
CGCAGTCTGT ATGCACATAT CCAGAAACTG TCTCAGGTCC CTCCGGGTGA GAGTGTTACC 
TCCATGGAAC TAGAGTTCAA GCTTTTGGCC AACTCTAAAG CCCACACCTC ACGATTTATC 
AGTGCCAACC TCCCATGCAA CAAGTTCAAA AACAGACTGG TAAACATCAT GCCATATGAA 
CTGAGTCGTG TCTCCCTGCA GCCCATTCGT GGTGTTGAGG GATCAGATTA TATCAATGCA 
AGTTTCATTG ATGGCTACAG GCAGCAGAAA GGCTACATTG CTACTCAGGG TCCATTGGCC 
GAGACCACTG AGGATTTCTG GAGAATGCTG TGGGAACATA ACTCCACCAT TGTGGTGATG 
CTGACAAAGT TGCGAGAAAT GGGGAGGGAG AAGTGCCATC AGTACTGGCC TGCAGAGCGC 
TCAGCACGGT ACCAGTATTT TGTGGTGGAC CCTATGGCTG AGTATAACAT GCCTCAGTAT 
ATCCTGCGTG AGTTCAAAGT GACAGATGCA CGGGATGGCC AATCTCGTAC AATTAGACAA 
TTCCAGTTCA CTGATTGGCC TGAACAAGGA GTGCCAAAAA CAGGTGAAGG CTTTATTGAC 
TTCATTGGGC AGGTGCACAA AACCAAGGAG CAGTTTGGGC AGGATGGGCC AATCACCGTT 
CACTGCAGCG CTGGCGTGGG CCGCACTGGA GTATTCATCA CTCTCAGTAT TGTACTGGAG 
CGTATGAGGT ACGAGGGTGT GGTTGACATG TTTCAAACAG TGAAGACTTT AAGAACACAG 
CGGCCAGCAA TGGTACAAAC AGAGGACCAG TATCAACTGT GCTATAGAGC CGCACTGGAG 
TATCTGGGCA GCTTTGACCA CTATGCAACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914020.2
CDS	636..6308
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKRKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_004913963.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913963.2

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTGAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACACT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGACATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCGAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTGACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTTCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA AGATGCTGTT ATCACTGGCC TCTCTCCTGA GACCACTTAT 
TCCATCACTG TGGCCGCCTA CACTACTAAG GGAGATGGTG CTAGAAGCAA ACCCAAGGTC 
GTTAGCACCA CAGGAGCTGT GCCTGGTAAA CCCACTATGA TGATCAGCAC CACAGCACTG 
AACACTGCTC TGATCCAGTG GCATCCTCCA AGAGAGATTG TGGGAGAGTT GTTAGGATAT 
CGTTTGCAGT ACAAGCGAGT GGATGAAGAC AGGTTTATTA CAATTGATTT TAGCAAGAAT 
GAACATCACC ACACAGTGAC TGGGCTACAT AAAGGTGCAA GCTATATATT CAAGCTTTCT 
GCAAAAAACC GAGCTGGAGC AGGGGAGGAA TATGAAAAGG AAATTTCCAC AGCTGAAGAT 
TTTCCTAGTG GGTTTCCCCA AAATCTGCAA GTAGTTGGAC TTACAACCTC TACTACAGAA 
GTGTCCTGGG AACCACCAGT GTTGTCTGAG CGCAATGGAA AGATCACCAA CTACACAGTG 
GTGTATCGGG ACATAAATAG CCAGCAGGAT CTCACCAATG TCACTCGCGA CACACACATA 
ACATTGGTCA ATCTCAAGCC AGACACCACA TATGACATCA AGGTCAGGGC AAGAACAAAC 
AAAGGCGCTG GCCCTCTAAG TCCTAGCATT CAATCACGTA CAATGCCTGT TGACCAAGTG 
TTTGCTAAGA ATTTCAGAGT AAACGCCGTG ACAAAAACCT CAGTCTTGCT AAGCTGGGAG 
CTGCCTGATT CTTACAAATC AGCTGTGCCT TTCAAGATCT TTTACAATAA TCAACATGTA 
GAAGTTGATG GACACTCTAT GCGCAAACTG ATCAGTAATC TCAAGCCAGA TACAGAATAC 
TCCTTTGTGC TGATGAACAG GGGGAGCAGT GCAGGTGGAC TGCAGCACCG CGTGTCCATT 
CGCACAGCAC CAGATGTTTT AAAAAGCAAG CCTTTTGCTG CCAACAAATA CATGGAGGAG 
GGCAGGTTCA CCTTGCTGTT GCCTCAGGTT CAGACTACTT CCCCAGTAAG GTGGTATTAT 
ATTGTGGTTG TCCCACTGGA CAGTTCCAGC GGGGGTCTGA ATTCTCGCTG GCAGAGCCCT 
GAGGAAATGG AGCTAGAGCA GATACTTGAA GCAATAAACC AGGGATCCCG TCGTCATAGA 
AGACAGGCAA GCAAGAATAA GCCATACATT GCAGCCCGAG TAAACACACT TCCAGAATCC 
TTTATTTTGG GTGACAATAA AGAATATAAT GGGTTCATCA ACAAGCCACT GAATCAGAAT 
TTACGCTACA CCTGCTTTGT GCTAGCTGCA CTAGAAGATG GGGACACTAA AAAGTATGCA 
GCCAGCCCAT ACTCAGATGA TATTGTGGTG GAGGTGATCT CACAGGCAAA ACAACAGGAA 
GAGCCAGAAA TGCTTTGGGT CATGGGTCCT GTTCTGGCTG TAATTCTTAT AATAATTATA 
GTTATTGCCA TTCTACTTTT CAAAAGGAAA AGGACCAACT CTCCTTCAGG CAAAGATGAA 
CAGTCAGTAG GACTTAAAGA CTGTCTCCTC GCTCACTCCT CAGATCCAGT TGAAATGAGG 
CGTCTAAACT ACCAAACTCC AGGTATGAGA GACCACCCTC CTATTCCAAT CTCTGACCTG 
GCAGACAACA TTGAAAGACT GAAGGCTAAT GATGGACTCA AGTTCTCTCA GGAATATGAG 
TCTATTGATC CAGGTCAGCA ATTCACGTGG GAAAATTCAA ATCTAGAAGT AAACAAACCA 
AAGAATCGCT ATGCAAATGT CATTGCATAT GACCATTCAA GGGTCATTCT TACCTCAGTG 
GATGGAGTTC CCGGAAGTGA TTACATTAAT GCCAATTACA TAGATGGGTA CAGGAAACAG 
AATGCCTATA TAGCTACGCA GGGCCCACTG CCAGAGACTA TCACTGATTT CTGGAGAATG 
GTGTGGGAGC AAAGAACCGC CACTATTGTC ATGATGACCC GGCTAGAGGA AAAATCAAGG 
GTGAAATGTG ACCAGTACTG GCCCGGTCGT GGCTCAGAGA CCTATGGTAT GATCCAAGTC 
ACATTAATGG ACACAGTAGA GCTTGCCACC TACACAGTTA GGACTTTCAC ACTTTATAAG 
AATGGCTCCA GTGAGAAGCG GGATGTCAGA CAGTTCCAAT TTATGGCCTG GCCTGATCAC 
GGGGTCCCTG AATATCCTAC TCCTATTCTT GGATTCCTTC GCAGAGTGAA AGGCTGTAAT 
CCACCTGATG CTGGTCCTAT GGTTGTTCAT TGCAGTGCTG GCGTGGGCAG AACAGGATGC 
TTTATTGTTA TTGATGCCAT GCTAGAACGG GTGAAACTGG AGAAAACTGT GGATATTTAT 
GGTCATGTGA CCTGCATGCG CTCCCAACGA AATTATATGG TGCAAACAGA GGACCAGTAC 
ATCTTTATCC ATGAGGCTTT GCTTGAAGCA GTAATGTGCG GGATCACTGA AGTGCCAGCA 
CGCAGTCTGT ATGCACATAT CCAGAAACTG TCTCAGGTCC CTCCGGGTGA GAGTGTTACC 
TCCATGGAAC TAGAGTTCAA GCTTTTGGCC AACTCTAAAG CCCACACCTC ACGATTTATC 
AGTGCCAACC TCCCATGCAA CAAGTTCAAA AACAGACTGG TAAACATCAT GCCATATGAA 
CTGAGTCGTG TCTCCCTGCA GCCCATTCGT GGTGTTGAGG GATCAGATTA TATCAATGCA 
AGTTTCATTG ATGGCTACAG GCAGCAGAAA GGCTACATTG CTACTCAGGG TCCATTGGCC 
GAGACCACTG AGGATTTCTG GAGAATGCTG TGGGAACATA ACTCCACCAT TGTGGTGATG 
CTGACAAAGT TGCGAGAAAT GGGGAGGGAG AAGTGCCATC AGTACTGGCC TGCAGAGCGC 
TCAGCACGGT ACCAGTATTT TGTGGTGGAC CCTATGGCTG AGTATAACAT GCCTCAGTAT 
ATCCTGCGTG AGTTCAAAGT GACAGATGCA CGGGATGGCC AATCTCGTAC AATTAGACAA 
TTCCAGTTCA CTGATTGGCC TGAACAAGGA GTGCCAAAAA CAGGTGAAGG CTTTATTGAC 
TTCATTGGGC AGGTGCACAA AACCAAGGAG CAGTTTGGGC AGGATGGGCC AATCACCGTT 
CACTGCAGCG CTGGCGTGGG CCGCACTGGA GTATTCATCA CTCTCAGTAT TGTACTGGAG 
CGTATGAGGT ACGAGGGTGT GGTTGACATG TTTCAAACAG TGAAGACTTT AAGAACACAG 
CGGCCAGCAA TGGTACAAAC AGAGGACCAG TATCAACTGT GCTATAGAGC CGCACTGGAG 
TATCTGGGCA GCTTTGACCA CTATGCAACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_031900931.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756791.1 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X12, mRNA.

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CloneID	OXa57138
Clone ID Related Accession (Same CDS sequence)	XM_031900931.1
Accession Version	XM_031900931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAT GCCGAGTGCA CCACCAAGAA AGGTTGAAGT AGACTCTGTA 
AACTCCACAT CTGTTCGTGT GACATGGAAG TCTCCTCTAC CAACCAAGCA AAATGGGCAA 
ATTAGGGGGT ATCAAGTCAC TTATGTTCGT CTAGAAGGTG GAGAGCCTCG GGGGAATCCC 
ATCATAAAAG ATGTTATGCT GTCAGAGGCT CAATGGAGAC CTGAGGATGT GGGAGATTAC 
GATGCTGTTA TCACTGGCCT CTCTCCTGAG ACCACTTATT CCATCACTGT GGCCGCCTAC 
ACTACTAAGG GAGATGGTGC TAGAAGCAAA CCCAAGGTCG TTAGCACCAC AGGAGCTGTG 
CCTGGTAAAC CCACTATGAT GATCAGCACC ACAGCACTGA ACACTGCTCT GATCCAGTGG 
CATCCTCCAA GAGAGATTGT GGGAGAGTTG TTAGGATATC GTTTGCAGTA CAAGCGAGTG 
GATGAAGACA GGTTTATTAC AATTGATTTT AGCAAGAATG AACATCACCA CACAGTGACT 
GGGCTACATA AAGGTGCAAG CTATATATTC AAGCTTTCTG CAAAAAACCG AGCTGGAGCA 
GGGGAGGAAT ATGAAAAGGA AATTTCCACA GCTGAAGATT TTCCTAGTGG GTTTCCCCAA 
AATCTGCAAG TAGTTGGACT TACAACCTCT ACTACAGAAG TGTCCTGGGA ACCACCAGTG 
TTGTCTGAGC GCAATGGAAA GATCACCAAC TACACAGTGG TGTATCGGGA CATAAATAGC 
CAGCAGGATC TCACCAATGT CACTCGCGAC ACACACATAA CATTGGTCAA TCTCAAGCCA 
GACACCACAT ATGACATCAA GGTCAGGGCA AGAACAAACA AAGGCGCTGG CCCTCTAAGT 
CCTAGCATTC AATCACGTAC AATGCCTGTT GACCAAGTGT TTGCTAAGAA TTTCAGAGTA 
AACGCCGTGA CAAAAACCTC AGTCTTGCTA AGCTGGGAGC TGCCTGATTC TTACAAATCA 
GCTGTGCCTT TCAAGATCTT TTACAATAAT CAACATGTAG AAGTTGATGG ACACTCTATG 
CGCAAACTGA TCAGTAATCT CAAGCCAGAT ACAGAATACT CCTTTGTGCT GATGAACAGG 
GGGAGCAGTG CAGGTGGACT GCAGCACCGC GTGTCCATTC GCACAGCACC AGATGTTTTA 
AAAAGCAAGC CTTTTGCTGC CAACAAATAC ATGGAGGAGG GCAGGTTCAC CTTGCTGTTG 
CCTCAGGTTC AGACTACTTC CCCAGTAAGG TGGTATTATA TTGTGGTTGT CCCACTGGAC 
AGTTCCAGCG GGGGTCTGAA TTCTCGCTGG CAGAGCCCTG AGGAAATGGA GCTAGAGCAG 
ATACTTGAAG CAATAAACCA GGGATCCCGT CGTCATAGAA GACAGGCAAG CAAGAATAAG 
CCATACATTG CAGCCCGAGT AAACACACTT CCAGAATCCT TTATTTTGGG TGACAATAAA 
GAATATAATG GGTTCATCAA CAAGCCACTG AATCAGAATT TACGCTACAC CTGCTTTGTG 
CTAGCTGCAC TAGAAGATGG GGACACTAAA AAGTATGCAG CCAGCCCATA CTCAGATGAT 
ATTGTGGTGG AGGTGATCTC ACAGGCAAAA CAACAGGAAG AGCCAGAAAT GCTTTGGGTC 
ATGGGTCCTG TTCTGGCTGT AATTCTTATA ATAATTATAG TTATTGCCAT TCTACTTTTC 
AAAAGTAAAC AGGAAAGGAA AAGGACCAAC TCTCCTTCAG GCAAAGATGA ACAGTCAGTA 
GGACTTAAAG ACTGTCTCCT CGCTCACTCC TCAGATCCAG TTGAAATGAG GCGTCTAAAC 
TACCAAACTC CAGGTTCCAG TGTCCCCAGT TACCCGAATA TCTCAAGTAT GAGAGACCAC 
CCTCCTATTC CAATCTCTGA CCTGGCAGAC AACATTGAAA GACTGAAGGC TAATGATGGA 
CTCAAGTTCT CTCAGGAATA TGAGTCTATT GATCCAGGTC AGCAATTCAC GTGGGAAAAT 
TCAAATCTAG AAGTAAACAA ACCAAAGAAT CGCTATGCAA ATGTCATTGC ATATGACCAT 
TCAAGGGTCA TTCTTACCTC AGTGGATGGA GTTCCCGGAA GTGATTACAT TAATGCCAAT 
TACATAGATG GGTACAGGAA ACAGAATGCC TATATAGCTA CGCAGGGCCC ACTGCCAGAG 
ACTATCACTG ATTTCTGGAG AATGGTGTGG GAGCAAAGAA CCGCCACTAT TGTCATGATG 
ACCCGGCTAG AGGAAAAATC AAGGGTGAAA TGTGACCAGT ACTGGCCCGG TCGTGGCTCA 
GAGACCTATG GTATGATCCA AGTCACATTA ATGGACACAG TAGAGCTTGC CACCTACACA 
GTTAGGACTT TCACACTTTA TAAGAATGGC TCCAGTGAGA AGCGGGATGT CAGACAGTTC 
CAATTTATGG CCTGGCCTGA TCACGGGGTC CCTGAATATC CTACTCCTAT TCTTGGATTC 
CTTCGCAGAG TGAAAGGCTG TAATCCACCT GATGCTGGTC CTATGGTTGT TCATTGCAGT 
GCTGGCGTGG GCAGAACAGG ATGCTTTATT GTTATTGATG CCATGCTAGA ACGGGTGAAA 
CTGGAGAAAA CTGTGGATAT TTATGGTCAT GTGACCTGCA TGCGCTCCCA ACGAAATTAT 
ATGGTGCAAA CAGAGGACCA GTACATCTTT ATCCATGAGG CTTTGCTTGA AGCAGTAATG 
TGCGGGATCA CTGAAGTGCC AGCACGCAGT CTGTATGCAC ATATCCAGAA ACTGTCTCAG 
GTCCCTCCGG GTGAGAGTGT TACCTCCATG GAACTAGAGT TCAAGCTTTT GGCCAACTCT 
AAAGCCCACA CCTCACGATT TATCAGTGCC AACCTCCCAT GCAACAAGTT CAAAAACAGA 
CTGGTAAACA TCATGCCATA TGAACTGAGT CGTGTCTCCC TGCAGCCCAT TCGTGGTGTT 
GAGGGATCAG ATTATATCAA TGCAAGTTTC ATTGATGGCT ACAGGCAGCA GAAAGGCTAC 
ATTGCTACTC AGGGTCCATT GGCCGAGACC ACTGAGGATT TCTGGAGAAT GCTGTGGGAA 
CATAACTCCA CCATTGTGGT GATGCTGACA AAGTTGCGAG AAATGGGGAG GGAGAAGTGC 
CATCAGTACT GGCCTGCAGA GCGCTCAGCA CGGTACCAGT ATTTTGTGGT GGACCCTATG 
GCTGAGTATA ACATGCCTCA GTATATCCTG CGTGAGTTCA AAGTGACAGA TGCACGGGAT 
GGCCAATCTC GTACAATTAG ACAATTCCAG TTCACTGATT GGCCTGAACA AGGAGTGCCA 
AAAACAGGTG AAGGCTTTAT TGACTTCATT GGGCAGGTGC ACAAAACCAA GGAGCAGTTT 
GGGCAGGATG GGCCAATCAC CGTTCACTGC AGCGCTGGCG TGGGCCGCAC TGGAGTATTC 
ATCACTCTCA GTATTGTACT GGAGCGTATG AGGTACGAGG GTGTGGTTGA CATGTTTCAA 
ACAGTGAAGA CTTTAAGAAC ACAGCGGCCA GCAATGGTAC AAACAGAGGA CCAGTATCAA 
CTGTGCTATA GAGCCGCACT GGAGTATCTG GGCAGCTTTG ACCACTATGC AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756791.1
CDS	636..6092
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSMPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQWRPEDVGDYDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKSKQERKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGSSVPSYPNISSMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_031900931.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900931.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAT GCCGAGTGCA CCACCAAGAA AGGTTGAAGT AGACTCTGTA 
AACTCCACAT CTGTTCGTGT GACATGGAAG TCTCCTCTAC CAACCAAGCA AAATGGGCAA 
ATTAGGGGGT ATCAAGTCAC TTATGTTCGT CTAGAAGGTG GAGAGCCTCG GGGGAATCCC 
ATCATAAAAG ATGTTATGCT GTCAGAGGCT CAATGGAGAC CTGAGGATGT GGGAGATTAC 
GATGCTGTTA TCACTGGCCT CTCTCCTGAG ACCACTTATT CCATCACTGT GGCCGCCTAC 
ACTACTAAGG GAGATGGTGC TAGAAGCAAA CCCAAGGTCG TTAGCACCAC AGGAGCTGTG 
CCTGGTAAAC CCACTATGAT GATCAGCACC ACAGCACTGA ACACTGCTCT GATCCAGTGG 
CATCCTCCAA GAGAGATTGT GGGAGAGTTG TTAGGATATC GTTTGCAGTA CAAGCGAGTG 
GATGAAGACA GGTTTATTAC AATTGATTTT AGCAAGAATG AACATCACCA CACAGTGACT 
GGGCTACATA AAGGTGCAAG CTATATATTC AAGCTTTCTG CAAAAAACCG AGCTGGAGCA 
GGGGAGGAAT ATGAAAAGGA AATTTCCACA GCTGAAGATT TTCCTAGTGG GTTTCCCCAA 
AATCTGCAAG TAGTTGGACT TACAACCTCT ACTACAGAAG TGTCCTGGGA ACCACCAGTG 
TTGTCTGAGC GCAATGGAAA GATCACCAAC TACACAGTGG TGTATCGGGA CATAAATAGC 
CAGCAGGATC TCACCAATGT CACTCGCGAC ACACACATAA CATTGGTCAA TCTCAAGCCA 
GACACCACAT ATGACATCAA GGTCAGGGCA AGAACAAACA AAGGCGCTGG CCCTCTAAGT 
CCTAGCATTC AATCACGTAC AATGCCTGTT GACCAAGTGT TTGCTAAGAA TTTCAGAGTA 
AACGCCGTGA CAAAAACCTC AGTCTTGCTA AGCTGGGAGC TGCCTGATTC TTACAAATCA 
GCTGTGCCTT TCAAGATCTT TTACAATAAT CAACATGTAG AAGTTGATGG ACACTCTATG 
CGCAAACTGA TCAGTAATCT CAAGCCAGAT ACAGAATACT CCTTTGTGCT GATGAACAGG 
GGGAGCAGTG CAGGTGGACT GCAGCACCGC GTGTCCATTC GCACAGCACC AGATGTTTTA 
AAAAGCAAGC CTTTTGCTGC CAACAAATAC ATGGAGGAGG GCAGGTTCAC CTTGCTGTTG 
CCTCAGGTTC AGACTACTTC CCCAGTAAGG TGGTATTATA TTGTGGTTGT CCCACTGGAC 
AGTTCCAGCG GGGGTCTGAA TTCTCGCTGG CAGAGCCCTG AGGAAATGGA GCTAGAGCAG 
ATACTTGAAG CAATAAACCA GGGATCCCGT CGTCATAGAA GACAGGCAAG CAAGAATAAG 
CCATACATTG CAGCCCGAGT AAACACACTT CCAGAATCCT TTATTTTGGG TGACAATAAA 
GAATATAATG GGTTCATCAA CAAGCCACTG AATCAGAATT TACGCTACAC CTGCTTTGTG 
CTAGCTGCAC TAGAAGATGG GGACACTAAA AAGTATGCAG CCAGCCCATA CTCAGATGAT 
ATTGTGGTGG AGGTGATCTC ACAGGCAAAA CAACAGGAAG AGCCAGAAAT GCTTTGGGTC 
ATGGGTCCTG TTCTGGCTGT AATTCTTATA ATAATTATAG TTATTGCCAT TCTACTTTTC 
AAAAGTAAAC AGGAAAGGAA AAGGACCAAC TCTCCTTCAG GCAAAGATGA ACAGTCAGTA 
GGACTTAAAG ACTGTCTCCT CGCTCACTCC TCAGATCCAG TTGAAATGAG GCGTCTAAAC 
TACCAAACTC CAGGTTCCAG TGTCCCCAGT TACCCGAATA TCTCAAGTAT GAGAGACCAC 
CCTCCTATTC CAATCTCTGA CCTGGCAGAC AACATTGAAA GACTGAAGGC TAATGATGGA 
CTCAAGTTCT CTCAGGAATA TGAGTCTATT GATCCAGGTC AGCAATTCAC GTGGGAAAAT 
TCAAATCTAG AAGTAAACAA ACCAAAGAAT CGCTATGCAA ATGTCATTGC ATATGACCAT 
TCAAGGGTCA TTCTTACCTC AGTGGATGGA GTTCCCGGAA GTGATTACAT TAATGCCAAT 
TACATAGATG GGTACAGGAA ACAGAATGCC TATATAGCTA CGCAGGGCCC ACTGCCAGAG 
ACTATCACTG ATTTCTGGAG AATGGTGTGG GAGCAAAGAA CCGCCACTAT TGTCATGATG 
ACCCGGCTAG AGGAAAAATC AAGGGTGAAA TGTGACCAGT ACTGGCCCGG TCGTGGCTCA 
GAGACCTATG GTATGATCCA AGTCACATTA ATGGACACAG TAGAGCTTGC CACCTACACA 
GTTAGGACTT TCACACTTTA TAAGAATGGC TCCAGTGAGA AGCGGGATGT CAGACAGTTC 
CAATTTATGG CCTGGCCTGA TCACGGGGTC CCTGAATATC CTACTCCTAT TCTTGGATTC 
CTTCGCAGAG TGAAAGGCTG TAATCCACCT GATGCTGGTC CTATGGTTGT TCATTGCAGT 
GCTGGCGTGG GCAGAACAGG ATGCTTTATT GTTATTGATG CCATGCTAGA ACGGGTGAAA 
CTGGAGAAAA CTGTGGATAT TTATGGTCAT GTGACCTGCA TGCGCTCCCA ACGAAATTAT 
ATGGTGCAAA CAGAGGACCA GTACATCTTT ATCCATGAGG CTTTGCTTGA AGCAGTAATG 
TGCGGGATCA CTGAAGTGCC AGCACGCAGT CTGTATGCAC ATATCCAGAA ACTGTCTCAG 
GTCCCTCCGG GTGAGAGTGT TACCTCCATG GAACTAGAGT TCAAGCTTTT GGCCAACTCT 
AAAGCCCACA CCTCACGATT TATCAGTGCC AACCTCCCAT GCAACAAGTT CAAAAACAGA 
CTGGTAAACA TCATGCCATA TGAACTGAGT CGTGTCTCCC TGCAGCCCAT TCGTGGTGTT 
GAGGGATCAG ATTATATCAA TGCAAGTTTC ATTGATGGCT ACAGGCAGCA GAAAGGCTAC 
ATTGCTACTC AGGGTCCATT GGCCGAGACC ACTGAGGATT TCTGGAGAAT GCTGTGGGAA 
CATAACTCCA CCATTGTGGT GATGCTGACA AAGTTGCGAG AAATGGGGAG GGAGAAGTGC 
CATCAGTACT GGCCTGCAGA GCGCTCAGCA CGGTACCAGT ATTTTGTGGT GGACCCTATG 
GCTGAGTATA ACATGCCTCA GTATATCCTG CGTGAGTTCA AAGTGACAGA TGCACGGGAT 
GGCCAATCTC GTACAATTAG ACAATTCCAG TTCACTGATT GGCCTGAACA AGGAGTGCCA 
AAAACAGGTG AAGGCTTTAT TGACTTCATT GGGCAGGTGC ACAAAACCAA GGAGCAGTTT 
GGGCAGGATG GGCCAATCAC CGTTCACTGC AGCGCTGGCG TGGGCCGCAC TGGAGTATTC 
ATCACTCTCA GTATTGTACT GGAGCGTATG AGGTACGAGG GTGTGGTTGA CATGTTTCAA 
ACAGTGAAGA CTTTAAGAAC ACAGCGGCCA GCAATGGTAC AAACAGAGGA CCAGTATCAA 
CTGTGCTATA GAGCCGCACT GGAGTATCTG GGCAGCTTTG ACCACTATGC AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_031900932.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756792.1 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X13, mRNA.

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CloneID	OXa57139
Clone ID Related Accession (Same CDS sequence)	XM_031900932.1
Accession Version	XM_031900932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTGAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACACT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGACATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ATGCCGAGTG CACCACCAAG AAAGGTTGAA GTAGACTCTG TAAACTCCAC ATCTGTTCGT 
GTGACATGGA AGTCTCCTCT ACCAACCAAG CAAAATGGGC AAATTAGGGG GTATCAAGTC 
ACTTATGTTC GTCTAGAAGG TGGAGAGCCT CGGGGGAATC CCATCATAAA AGATGTTATG 
CTGTCAGAGG CTCAATGGAG ACCTGAGGAT GTGGGAGATT ACGATGCTGT TATCACTGGC 
CTCTCTCCTG AGACCACTTA TTCCATCACT GTGGCCGCCT ACACTACTAA GGGAGATGGT 
GCTAGAAGCA AACCCAAGGT CGTTAGCACC ACAGGAGCTG TGCCTGGTAA ACCCACTATG 
ATGATCAGCA CCACAGCACT GAACACTGCT CTGATCCAGT GGCATCCTCC AAGAGAGATT 
GTGGGAGAGT TGTTAGGATA TCGTTTGCAG TACAAGCGAG TGGATGAAGA CAGGTTTATT 
ACAATTGATT TTAGCAAGAA TGAACATCAC CACACAGTGA CTGGGCTACA TAAAGGTGCA 
AGCTATATAT TCAAGCTTTC TGCAAAAAAC CGAGCTGGAG CAGGGGAGGA ATATGAAAAG 
GAAATTTCCA CAGCTGAAGA TTTTCCTAGT GGGTTTCCCC AAAATCTGCA AGTAGTTGGA 
CTTACAACCT CTACTACAGA AGTGTCCTGG GAACCACCAG TGTTGTCTGA GCGCAATGGA 
AAGATCACCA ACTACACAGT GGTGTATCGG GACATAAATA GCCAGCAGGA TCTCACCAAT 
GTCACTCGCG ACACACACAT AACATTGGTC AATCTCAAGC CAGACACCAC ATATGACATC 
AAGGTCAGGG CAAGAACAAA CAAAGGCGCT GGCCCTCTAA GTCCTAGCAT TCAATCACGT 
ACAATGCCTG TTGACCAAGT GTTTGCTAAG AATTTCAGAG TAAACGCCGT GACAAAAACC 
TCAGTCTTGC TAAGCTGGGA GCTGCCTGAT TCTTACAAAT CAGCTGTGCC TTTCAAGATC 
TTTTACAATA ATCAACATGT AGAAGTTGAT GGACACTCTA TGCGCAAACT GATCAGTAAT 
CTCAAGCCAG ATACAGAATA CTCCTTTGTG CTGATGAACA GGGGGAGCAG TGCAGGTGGA 
CTGCAGCACC GCGTGTCCAT TCGCACAGCA CCAGATGTTT TAAAAAGCAA GCCTTTTGCT 
GCCAACAAAT ACATGGAGGA GGGCAGGTTC ACCTTGCTGT TGCCTCAGGT TCAGACTACT 
TCCCCAGTAA GGTGGTATTA TATTGTGGTT GTCCCACTGG ACAGTTCCAG CGGGGGTCTG 
AATTCTCGCT GGCAGAGCCC TGAGGAAATG GAGCTAGAGC AGATACTTGA AGCAATAAAC 
CAGGGATCCC GTCGTCATAG AAGACAGGCA AGCAAGAATA AGCCATACAT TGCAGCCCGA 
GTAAACACAC TTCCAGAATC CTTTATTTTG GGTGACAATA AAGAATATAA TGGGTTCATC 
AACAAGCCAC TGAATCAGAA TTTACGCTAC ACCTGCTTTG TGCTAGCTGC ACTAGAAGAT 
GGGGACACTA AAAAGTATGC AGCCAGCCCA TACTCAGATG ATATTGTGGT GGAGGTGATC 
TCACAGGCAA AACAACAGGA AGAGCCAGAA ATGCTTTGGG TCATGGGTCC TGTTCTGGCT 
GTAATTCTTA TAATAATTAT AGTTATTGCC ATTCTACTTT TCAAAAGTAA ACAGGAAAGG 
AAAAGGACCA ACTCTCCTTC AGGCAAAGAT GAACAGTCAG TAGGACTTAA AGACTGTCTC 
CTCGCTCACT CCTCAGATCC AGTTGAAATG AGGCGTCTAA ACTACCAAAC TCCAGGTTCC 
AGTGTCCCCA GTTACCCGAA TATCTCAAGT ATGAGAGACC ACCCTCCTAT TCCAATCTCT 
GACCTGGCAG ACAACATTGA AAGACTGAAG GCTAATGATG GACTCAAGTT CTCTCAGGAA 
TATGAGTCTA TTGATCCAGG TCAGCAATTC ACGTGGGAAA ATTCAAATCT AGAAGTAAAC 
AAACCAAAGA ATCGCTATGC AAATGTCATT GCATATGACC ATTCAAGGGT CATTCTTACC 
TCAGTGGATG GAGTTCCCGG AAGTGATTAC ATTAATGCCA ATTACATAGA TGGGTACAGG 
AAACAGAATG CCTATATAGC TACGCAGGGC CCACTGCCAG AGACTATCAC TGATTTCTGG 
AGAATGGTGT GGGAGCAAAG AACCGCCACT ATTGTCATGA TGACCCGGCT AGAGGAAAAA 
TCAAGGGTGA AATGTGACCA GTACTGGCCC GGTCGTGGCT CAGAGACCTA TGGTATGATC 
CAAGTCACAT TAATGGACAC AGTAGAGCTT GCCACCTACA CAGTTAGGAC TTTCACACTT 
TATAAGAATG GCTCCAGTGA GAAGCGGGAT GTCAGACAGT TCCAATTTAT GGCCTGGCCT 
GATCACGGGG TCCCTGAATA TCCTACTCCT ATTCTTGGAT TCCTTCGCAG AGTGAAAGGC 
TGTAATCCAC CTGATGCTGG TCCTATGGTT GTTCATTGCA GTGCTGGCGT GGGCAGAACA 
GGATGCTTTA TTGTTATTGA TGCCATGCTA GAACGGGTGA AACTGGAGAA AACTGTGGAT 
ATTTATGGTC ATGTGACCTG CATGCGCTCC CAACGAAATT ATATGGTGCA AACAGAGGAC 
CAGTACATCT TTATCCATGA GGCTTTGCTT GAAGCAGTAA TGTGCGGGAT CACTGAAGTG 
CCAGCACGCA GTCTGTATGC ACATATCCAG AAACTGTCTC AGGTCCCTCC GGGTGAGAGT 
GTTACCTCCA TGGAACTAGA GTTCAAGCTT TTGGCCAACT CTAAAGCCCA CACCTCACGA 
TTTATCAGTG CCAACCTCCC ATGCAACAAG TTCAAAAACA GACTGGTAAA CATCATGCCA 
TATGAACTGA GTCGTGTCTC CCTGCAGCCC ATTCGTGGTG TTGAGGGATC AGATTATATC 
AATGCAAGTT TCATTGATGG CTACAGGCAG CAGAAAGGCT ACATTGCTAC TCAGGGTCCA 
TTGGCCGAGA CCACTGAGGA TTTCTGGAGA ATGCTGTGGG AACATAACTC CACCATTGTG 
GTGATGCTGA CAAAGTTGCG AGAAATGGGG AGGGAGAAGT GCCATCAGTA CTGGCCTGCA 
GAGCGCTCAG CACGGTACCA GTATTTTGTG GTGGACCCTA TGGCTGAGTA TAACATGCCT 
CAGTATATCC TGCGTGAGTT CAAAGTGACA GATGCACGGG ATGGCCAATC TCGTACAATT 
AGACAATTCC AGTTCACTGA TTGGCCTGAA CAAGGAGTGC CAAAAACAGG TGAAGGCTTT 
ATTGACTTCA TTGGGCAGGT GCACAAAACC AAGGAGCAGT TTGGGCAGGA TGGGCCAATC 
ACCGTTCACT GCAGCGCTGG CGTGGGCCGC ACTGGAGTAT TCATCACTCT CAGTATTGTA 
CTGGAGCGTA TGAGGTACGA GGGTGTGGTT GACATGTTTC AAACAGTGAA GACTTTAAGA 
ACACAGCGGC CAGCAATGGT ACAAACAGAG GACCAGTATC AACTGTGCTA TAGAGCCGCA 
CTGGAGTATC TGGGCAGCTT TGACCACTAT GCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756792.1
CDS	636..6074
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSMPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQWRPEDVGDYDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKSKQERKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGSSVPSYPNISSMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_031900932.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900932.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTGAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACACT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGACATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ATGCCGAGTG CACCACCAAG AAAGGTTGAA GTAGACTCTG TAAACTCCAC ATCTGTTCGT 
GTGACATGGA AGTCTCCTCT ACCAACCAAG CAAAATGGGC AAATTAGGGG GTATCAAGTC 
ACTTATGTTC GTCTAGAAGG TGGAGAGCCT CGGGGGAATC CCATCATAAA AGATGTTATG 
CTGTCAGAGG CTCAATGGAG ACCTGAGGAT GTGGGAGATT ACGATGCTGT TATCACTGGC 
CTCTCTCCTG AGACCACTTA TTCCATCACT GTGGCCGCCT ACACTACTAA GGGAGATGGT 
GCTAGAAGCA AACCCAAGGT CGTTAGCACC ACAGGAGCTG TGCCTGGTAA ACCCACTATG 
ATGATCAGCA CCACAGCACT GAACACTGCT CTGATCCAGT GGCATCCTCC AAGAGAGATT 
GTGGGAGAGT TGTTAGGATA TCGTTTGCAG TACAAGCGAG TGGATGAAGA CAGGTTTATT 
ACAATTGATT TTAGCAAGAA TGAACATCAC CACACAGTGA CTGGGCTACA TAAAGGTGCA 
AGCTATATAT TCAAGCTTTC TGCAAAAAAC CGAGCTGGAG CAGGGGAGGA ATATGAAAAG 
GAAATTTCCA CAGCTGAAGA TTTTCCTAGT GGGTTTCCCC AAAATCTGCA AGTAGTTGGA 
CTTACAACCT CTACTACAGA AGTGTCCTGG GAACCACCAG TGTTGTCTGA GCGCAATGGA 
AAGATCACCA ACTACACAGT GGTGTATCGG GACATAAATA GCCAGCAGGA TCTCACCAAT 
GTCACTCGCG ACACACACAT AACATTGGTC AATCTCAAGC CAGACACCAC ATATGACATC 
AAGGTCAGGG CAAGAACAAA CAAAGGCGCT GGCCCTCTAA GTCCTAGCAT TCAATCACGT 
ACAATGCCTG TTGACCAAGT GTTTGCTAAG AATTTCAGAG TAAACGCCGT GACAAAAACC 
TCAGTCTTGC TAAGCTGGGA GCTGCCTGAT TCTTACAAAT CAGCTGTGCC TTTCAAGATC 
TTTTACAATA ATCAACATGT AGAAGTTGAT GGACACTCTA TGCGCAAACT GATCAGTAAT 
CTCAAGCCAG ATACAGAATA CTCCTTTGTG CTGATGAACA GGGGGAGCAG TGCAGGTGGA 
CTGCAGCACC GCGTGTCCAT TCGCACAGCA CCAGATGTTT TAAAAAGCAA GCCTTTTGCT 
GCCAACAAAT ACATGGAGGA GGGCAGGTTC ACCTTGCTGT TGCCTCAGGT TCAGACTACT 
TCCCCAGTAA GGTGGTATTA TATTGTGGTT GTCCCACTGG ACAGTTCCAG CGGGGGTCTG 
AATTCTCGCT GGCAGAGCCC TGAGGAAATG GAGCTAGAGC AGATACTTGA AGCAATAAAC 
CAGGGATCCC GTCGTCATAG AAGACAGGCA AGCAAGAATA AGCCATACAT TGCAGCCCGA 
GTAAACACAC TTCCAGAATC CTTTATTTTG GGTGACAATA AAGAATATAA TGGGTTCATC 
AACAAGCCAC TGAATCAGAA TTTACGCTAC ACCTGCTTTG TGCTAGCTGC ACTAGAAGAT 
GGGGACACTA AAAAGTATGC AGCCAGCCCA TACTCAGATG ATATTGTGGT GGAGGTGATC 
TCACAGGCAA AACAACAGGA AGAGCCAGAA ATGCTTTGGG TCATGGGTCC TGTTCTGGCT 
GTAATTCTTA TAATAATTAT AGTTATTGCC ATTCTACTTT TCAAAAGTAA ACAGGAAAGG 
AAAAGGACCA ACTCTCCTTC AGGCAAAGAT GAACAGTCAG TAGGACTTAA AGACTGTCTC 
CTCGCTCACT CCTCAGATCC AGTTGAAATG AGGCGTCTAA ACTACCAAAC TCCAGGTTCC 
AGTGTCCCCA GTTACCCGAA TATCTCAAGT ATGAGAGACC ACCCTCCTAT TCCAATCTCT 
GACCTGGCAG ACAACATTGA AAGACTGAAG GCTAATGATG GACTCAAGTT CTCTCAGGAA 
TATGAGTCTA TTGATCCAGG TCAGCAATTC ACGTGGGAAA ATTCAAATCT AGAAGTAAAC 
AAACCAAAGA ATCGCTATGC AAATGTCATT GCATATGACC ATTCAAGGGT CATTCTTACC 
TCAGTGGATG GAGTTCCCGG AAGTGATTAC ATTAATGCCA ATTACATAGA TGGGTACAGG 
AAACAGAATG CCTATATAGC TACGCAGGGC CCACTGCCAG AGACTATCAC TGATTTCTGG 
AGAATGGTGT GGGAGCAAAG AACCGCCACT ATTGTCATGA TGACCCGGCT AGAGGAAAAA 
TCAAGGGTGA AATGTGACCA GTACTGGCCC GGTCGTGGCT CAGAGACCTA TGGTATGATC 
CAAGTCACAT TAATGGACAC AGTAGAGCTT GCCACCTACA CAGTTAGGAC TTTCACACTT 
TATAAGAATG GCTCCAGTGA GAAGCGGGAT GTCAGACAGT TCCAATTTAT GGCCTGGCCT 
GATCACGGGG TCCCTGAATA TCCTACTCCT ATTCTTGGAT TCCTTCGCAG AGTGAAAGGC 
TGTAATCCAC CTGATGCTGG TCCTATGGTT GTTCATTGCA GTGCTGGCGT GGGCAGAACA 
GGATGCTTTA TTGTTATTGA TGCCATGCTA GAACGGGTGA AACTGGAGAA AACTGTGGAT 
ATTTATGGTC ATGTGACCTG CATGCGCTCC CAACGAAATT ATATGGTGCA AACAGAGGAC 
CAGTACATCT TTATCCATGA GGCTTTGCTT GAAGCAGTAA TGTGCGGGAT CACTGAAGTG 
CCAGCACGCA GTCTGTATGC ACATATCCAG AAACTGTCTC AGGTCCCTCC GGGTGAGAGT 
GTTACCTCCA TGGAACTAGA GTTCAAGCTT TTGGCCAACT CTAAAGCCCA CACCTCACGA 
TTTATCAGTG CCAACCTCCC ATGCAACAAG TTCAAAAACA GACTGGTAAA CATCATGCCA 
TATGAACTGA GTCGTGTCTC CCTGCAGCCC ATTCGTGGTG TTGAGGGATC AGATTATATC 
AATGCAAGTT TCATTGATGG CTACAGGCAG CAGAAAGGCT ACATTGCTAC TCAGGGTCCA 
TTGGCCGAGA CCACTGAGGA TTTCTGGAGA ATGCTGTGGG AACATAACTC CACCATTGTG 
GTGATGCTGA CAAAGTTGCG AGAAATGGGG AGGGAGAAGT GCCATCAGTA CTGGCCTGCA 
GAGCGCTCAG CACGGTACCA GTATTTTGTG GTGGACCCTA TGGCTGAGTA TAACATGCCT 
CAGTATATCC TGCGTGAGTT CAAAGTGACA GATGCACGGG ATGGCCAATC TCGTACAATT 
AGACAATTCC AGTTCACTGA TTGGCCTGAA CAAGGAGTGC CAAAAACAGG TGAAGGCTTT 
ATTGACTTCA TTGGGCAGGT GCACAAAACC AAGGAGCAGT TTGGGCAGGA TGGGCCAATC 
ACCGTTCACT GCAGCGCTGG CGTGGGCCGC ACTGGAGTAT TCATCACTCT CAGTATTGTA 
CTGGAGCGTA TGAGGTACGA GGGTGTGGTT GACATGTTTC AAACAGTGAA GACTTTAAGA 
ACACAGCGGC CAGCAATGGT ACAAACAGAG GACCAGTATC AACTGTGCTA TAGAGCCGCA 
CTGGAGTATC TGGGCAGCTT TGACCACTAT GCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_031900933.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756793.1 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X14, mRNA.

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CloneID	OXa57140
Clone ID Related Accession (Same CDS sequence)	XM_031900933.1
Accession Version	XM_031900933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTGAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACACT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGACATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ATGCCGAGTG CACCACCAAG AAAGGTTGAA GTAGACTCTG TAAACTCCAC ATCTGTTCGT 
GTGACATGGA AGTCTCCTCT ACCAACCAAG CAAAATGGGC AAATTAGGGG GTATCAAGTC 
ACTTATGTTC GTCTAGAAGG TGGAGAGCCT CGGGGGAATC CCATCATAAA AGATGTTATG 
CTGTCAGAGG CTCAAGATGC TGTTATCACT GGCCTCTCTC CTGAGACCAC TTATTCCATC 
ACTGTGGCCG CCTACACTAC TAAGGGAGAT GGTGCTAGAA GCAAACCCAA GGTCGTTAGC 
ACCACAGGAG CTGTGCCTGG TAAACCCACT ATGATGATCA GCACCACAGC ACTGAACACT 
GCTCTGATCC AGTGGCATCC TCCAAGAGAG ATTGTGGGAG AGTTGTTAGG ATATCGTTTG 
CAGTACAAGC GAGTGGATGA AGACAGGTTT ATTACAATTG ATTTTAGCAA GAATGAACAT 
CACCACACAG TGACTGGGCT ACATAAAGGT GCAAGCTATA TATTCAAGCT TTCTGCAAAA 
AACCGAGCTG GAGCAGGGGA GGAATATGAA AAGGAAATTT CCACAGCTGA AGATTTTCCT 
AGTGGGTTTC CCCAAAATCT GCAAGTAGTT GGACTTACAA CCTCTACTAC AGAAGTGTCC 
TGGGAACCAC CAGTGTTGTC TGAGCGCAAT GGAAAGATCA CCAACTACAC AGTGGTGTAT 
CGGGACATAA ATAGCCAGCA GGATCTCACC AATGTCACTC GCGACACACA CATAACATTG 
GTCAATCTCA AGCCAGACAC CACATATGAC ATCAAGGTCA GGGCAAGAAC AAACAAAGGC 
GCTGGCCCTC TAAGTCCTAG CATTCAATCA CGTACAATGC CTGTTGACCA AGTGTTTGCT 
AAGAATTTCA GAGTAAACGC CGTGACAAAA ACCTCAGTCT TGCTAAGCTG GGAGCTGCCT 
GATTCTTACA AATCAGCTGT GCCTTTCAAG ATCTTTTACA ATAATCAACA TGTAGAAGTT 
GATGGACACT CTATGCGCAA ACTGATCAGT AATCTCAAGC CAGATACAGA ATACTCCTTT 
GTGCTGATGA ACAGGGGGAG CAGTGCAGGT GGACTGCAGC ACCGCGTGTC CATTCGCACA 
GCACCAGATG TTTTAAAAAG CAAGCCTTTT GCTGCCAACA AATACATGGA GGAGGGCAGG 
TTCACCTTGC TGTTGCCTCA GGTTCAGACT ACTTCCCCAG TAAGGTGGTA TTATATTGTG 
GTTGTCCCAC TGGACAGTTC CAGCGGGGGT CTGAATTCTC GCTGGCAGAG CCCTGAGGAA 
ATGGAGCTAG AGCAGATACT TGAAGCAATA AACCAGGGAT CCCGTCGTCA TAGAAGACAG 
GCAAGCAAGA ATAAGCCATA CATTGCAGCC CGAGTAAACA CACTTCCAGA ATCCTTTATT 
TTGGGTGACA ATAAAGAATA TAATGGGTTC ATCAACAAGC CACTGAATCA GAATTTACGC 
TACACCTGCT TTGTGCTAGC TGCACTAGAA GATGGGGACA CTAAAAAGTA TGCAGCCAGC 
CCATACTCAG ATGATATTGT GGTGGAGGTG ATCTCACAGG CAAAACAACA GGAAGAGCCA 
GAAATGCTTT GGGTCATGGG TCCTGTTCTG GCTGTAATTC TTATAATAAT TATAGTTATT 
GCCATTCTAC TTTTCAAAAG TAAACAGGAA AGGAAAAGGA CCAACTCTCC TTCAGGCAAA 
GATGAACAGT CAGTAGGACT TAAAGACTGT CTCCTCGCTC ACTCCTCAGA TCCAGTTGAA 
ATGAGGCGTC TAAACTACCA AACTCCAGGT TCCAGTGTCC CCAGTTACCC GAATATCTCA 
AGTATGAGAG ACCACCCTCC TATTCCAATC TCTGACCTGG CAGACAACAT TGAAAGACTG 
AAGGCTAATG ATGGACTCAA GTTCTCTCAG GAATATGAGT CTATTGATCC AGGTCAGCAA 
TTCACGTGGG AAAATTCAAA TCTAGAAGTA AACAAACCAA AGAATCGCTA TGCAAATGTC 
ATTGCATATG ACCATTCAAG GGTCATTCTT ACCTCAGTGG ATGGAGTTCC CGGAAGTGAT 
TACATTAATG CCAATTACAT AGATGGGTAC AGGAAACAGA ATGCCTATAT AGCTACGCAG 
GGCCCACTGC CAGAGACTAT CACTGATTTC TGGAGAATGG TGTGGGAGCA AAGAACCGCC 
ACTATTGTCA TGATGACCCG GCTAGAGGAA AAATCAAGGG TGAAATGTGA CCAGTACTGG 
CCCGGTCGTG GCTCAGAGAC CTATGGTATG ATCCAAGTCA CATTAATGGA CACAGTAGAG 
CTTGCCACCT ACACAGTTAG GACTTTCACA CTTTATAAGA ATGGCTCCAG TGAGAAGCGG 
GATGTCAGAC AGTTCCAATT TATGGCCTGG CCTGATCACG GGGTCCCTGA ATATCCTACT 
CCTATTCTTG GATTCCTTCG CAGAGTGAAA GGCTGTAATC CACCTGATGC TGGTCCTATG 
GTTGTTCATT GCAGTGCTGG CGTGGGCAGA ACAGGATGCT TTATTGTTAT TGATGCCATG 
CTAGAACGGG TGAAACTGGA GAAAACTGTG GATATTTATG GTCATGTGAC CTGCATGCGC 
TCCCAACGAA ATTATATGGT GCAAACAGAG GACCAGTACA TCTTTATCCA TGAGGCTTTG 
CTTGAAGCAG TAATGTGCGG GATCACTGAA GTGCCAGCAC GCAGTCTGTA TGCACATATC 
CAGAAACTGT CTCAGGTCCC TCCGGGTGAG AGTGTTACCT CCATGGAACT AGAGTTCAAG 
CTTTTGGCCA ACTCTAAAGC CCACACCTCA CGATTTATCA GTGCCAACCT CCCATGCAAC 
AAGTTCAAAA ACAGACTGGT AAACATCATG CCATATGAAC TGAGTCGTGT CTCCCTGCAG 
CCCATTCGTG GTGTTGAGGG ATCAGATTAT ATCAATGCAA GTTTCATTGA TGGCTACAGG 
CAGCAGAAAG GCTACATTGC TACTCAGGGT CCATTGGCCG AGACCACTGA GGATTTCTGG 
AGAATGCTGT GGGAACATAA CTCCACCATT GTGGTGATGC TGACAAAGTT GCGAGAAATG 
GGGAGGGAGA AGTGCCATCA GTACTGGCCT GCAGAGCGCT CAGCACGGTA CCAGTATTTT 
GTGGTGGACC CTATGGCTGA GTATAACATG CCTCAGTATA TCCTGCGTGA GTTCAAAGTG 
ACAGATGCAC GGGATGGCCA ATCTCGTACA ATTAGACAAT TCCAGTTCAC TGATTGGCCT 
GAACAAGGAG TGCCAAAAAC AGGTGAAGGC TTTATTGACT TCATTGGGCA GGTGCACAAA 
ACCAAGGAGC AGTTTGGGCA GGATGGGCCA ATCACCGTTC ACTGCAGCGC TGGCGTGGGC 
CGCACTGGAG TATTCATCAC TCTCAGTATT GTACTGGAGC GTATGAGGTA CGAGGGTGTG 
GTTGACATGT TTCAAACAGT GAAGACTTTA AGAACACAGC GGCCAGCAAT GGTACAAACA 
GAGGACCAGT ATCAACTGTG CTATAGAGCC GCACTGGAGT ATCTGGGCAG CTTTGACCAC 
TATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756793.1
CDS	636..6047
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSMPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKSKQERKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGSSVPSYPNISSMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_031900933.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900933.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTGAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACACT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGACATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ATGCCGAGTG CACCACCAAG AAAGGTTGAA GTAGACTCTG TAAACTCCAC ATCTGTTCGT 
GTGACATGGA AGTCTCCTCT ACCAACCAAG CAAAATGGGC AAATTAGGGG GTATCAAGTC 
ACTTATGTTC GTCTAGAAGG TGGAGAGCCT CGGGGGAATC CCATCATAAA AGATGTTATG 
CTGTCAGAGG CTCAAGATGC TGTTATCACT GGCCTCTCTC CTGAGACCAC TTATTCCATC 
ACTGTGGCCG CCTACACTAC TAAGGGAGAT GGTGCTAGAA GCAAACCCAA GGTCGTTAGC 
ACCACAGGAG CTGTGCCTGG TAAACCCACT ATGATGATCA GCACCACAGC ACTGAACACT 
GCTCTGATCC AGTGGCATCC TCCAAGAGAG ATTGTGGGAG AGTTGTTAGG ATATCGTTTG 
CAGTACAAGC GAGTGGATGA AGACAGGTTT ATTACAATTG ATTTTAGCAA GAATGAACAT 
CACCACACAG TGACTGGGCT ACATAAAGGT GCAAGCTATA TATTCAAGCT TTCTGCAAAA 
AACCGAGCTG GAGCAGGGGA GGAATATGAA AAGGAAATTT CCACAGCTGA AGATTTTCCT 
AGTGGGTTTC CCCAAAATCT GCAAGTAGTT GGACTTACAA CCTCTACTAC AGAAGTGTCC 
TGGGAACCAC CAGTGTTGTC TGAGCGCAAT GGAAAGATCA CCAACTACAC AGTGGTGTAT 
CGGGACATAA ATAGCCAGCA GGATCTCACC AATGTCACTC GCGACACACA CATAACATTG 
GTCAATCTCA AGCCAGACAC CACATATGAC ATCAAGGTCA GGGCAAGAAC AAACAAAGGC 
GCTGGCCCTC TAAGTCCTAG CATTCAATCA CGTACAATGC CTGTTGACCA AGTGTTTGCT 
AAGAATTTCA GAGTAAACGC CGTGACAAAA ACCTCAGTCT TGCTAAGCTG GGAGCTGCCT 
GATTCTTACA AATCAGCTGT GCCTTTCAAG ATCTTTTACA ATAATCAACA TGTAGAAGTT 
GATGGACACT CTATGCGCAA ACTGATCAGT AATCTCAAGC CAGATACAGA ATACTCCTTT 
GTGCTGATGA ACAGGGGGAG CAGTGCAGGT GGACTGCAGC ACCGCGTGTC CATTCGCACA 
GCACCAGATG TTTTAAAAAG CAAGCCTTTT GCTGCCAACA AATACATGGA GGAGGGCAGG 
TTCACCTTGC TGTTGCCTCA GGTTCAGACT ACTTCCCCAG TAAGGTGGTA TTATATTGTG 
GTTGTCCCAC TGGACAGTTC CAGCGGGGGT CTGAATTCTC GCTGGCAGAG CCCTGAGGAA 
ATGGAGCTAG AGCAGATACT TGAAGCAATA AACCAGGGAT CCCGTCGTCA TAGAAGACAG 
GCAAGCAAGA ATAAGCCATA CATTGCAGCC CGAGTAAACA CACTTCCAGA ATCCTTTATT 
TTGGGTGACA ATAAAGAATA TAATGGGTTC ATCAACAAGC CACTGAATCA GAATTTACGC 
TACACCTGCT TTGTGCTAGC TGCACTAGAA GATGGGGACA CTAAAAAGTA TGCAGCCAGC 
CCATACTCAG ATGATATTGT GGTGGAGGTG ATCTCACAGG CAAAACAACA GGAAGAGCCA 
GAAATGCTTT GGGTCATGGG TCCTGTTCTG GCTGTAATTC TTATAATAAT TATAGTTATT 
GCCATTCTAC TTTTCAAAAG TAAACAGGAA AGGAAAAGGA CCAACTCTCC TTCAGGCAAA 
GATGAACAGT CAGTAGGACT TAAAGACTGT CTCCTCGCTC ACTCCTCAGA TCCAGTTGAA 
ATGAGGCGTC TAAACTACCA AACTCCAGGT TCCAGTGTCC CCAGTTACCC GAATATCTCA 
AGTATGAGAG ACCACCCTCC TATTCCAATC TCTGACCTGG CAGACAACAT TGAAAGACTG 
AAGGCTAATG ATGGACTCAA GTTCTCTCAG GAATATGAGT CTATTGATCC AGGTCAGCAA 
TTCACGTGGG AAAATTCAAA TCTAGAAGTA AACAAACCAA AGAATCGCTA TGCAAATGTC 
ATTGCATATG ACCATTCAAG GGTCATTCTT ACCTCAGTGG ATGGAGTTCC CGGAAGTGAT 
TACATTAATG CCAATTACAT AGATGGGTAC AGGAAACAGA ATGCCTATAT AGCTACGCAG 
GGCCCACTGC CAGAGACTAT CACTGATTTC TGGAGAATGG TGTGGGAGCA AAGAACCGCC 
ACTATTGTCA TGATGACCCG GCTAGAGGAA AAATCAAGGG TGAAATGTGA CCAGTACTGG 
CCCGGTCGTG GCTCAGAGAC CTATGGTATG ATCCAAGTCA CATTAATGGA CACAGTAGAG 
CTTGCCACCT ACACAGTTAG GACTTTCACA CTTTATAAGA ATGGCTCCAG TGAGAAGCGG 
GATGTCAGAC AGTTCCAATT TATGGCCTGG CCTGATCACG GGGTCCCTGA ATATCCTACT 
CCTATTCTTG GATTCCTTCG CAGAGTGAAA GGCTGTAATC CACCTGATGC TGGTCCTATG 
GTTGTTCATT GCAGTGCTGG CGTGGGCAGA ACAGGATGCT TTATTGTTAT TGATGCCATG 
CTAGAACGGG TGAAACTGGA GAAAACTGTG GATATTTATG GTCATGTGAC CTGCATGCGC 
TCCCAACGAA ATTATATGGT GCAAACAGAG GACCAGTACA TCTTTATCCA TGAGGCTTTG 
CTTGAAGCAG TAATGTGCGG GATCACTGAA GTGCCAGCAC GCAGTCTGTA TGCACATATC 
CAGAAACTGT CTCAGGTCCC TCCGGGTGAG AGTGTTACCT CCATGGAACT AGAGTTCAAG 
CTTTTGGCCA ACTCTAAAGC CCACACCTCA CGATTTATCA GTGCCAACCT CCCATGCAAC 
AAGTTCAAAA ACAGACTGGT AAACATCATG CCATATGAAC TGAGTCGTGT CTCCCTGCAG 
CCCATTCGTG GTGTTGAGGG ATCAGATTAT ATCAATGCAA GTTTCATTGA TGGCTACAGG 
CAGCAGAAAG GCTACATTGC TACTCAGGGT CCATTGGCCG AGACCACTGA GGATTTCTGG 
AGAATGCTGT GGGAACATAA CTCCACCATT GTGGTGATGC TGACAAAGTT GCGAGAAATG 
GGGAGGGAGA AGTGCCATCA GTACTGGCCT GCAGAGCGCT CAGCACGGTA CCAGTATTTT 
GTGGTGGACC CTATGGCTGA GTATAACATG CCTCAGTATA TCCTGCGTGA GTTCAAAGTG 
ACAGATGCAC GGGATGGCCA ATCTCGTACA ATTAGACAAT TCCAGTTCAC TGATTGGCCT 
GAACAAGGAG TGCCAAAAAC AGGTGAAGGC TTTATTGACT TCATTGGGCA GGTGCACAAA 
ACCAAGGAGC AGTTTGGGCA GGATGGGCCA ATCACCGTTC ACTGCAGCGC TGGCGTGGGC 
CGCACTGGAG TATTCATCAC TCTCAGTATT GTACTGGAGC GTATGAGGTA CGAGGGTGTG 
GTTGACATGT TTCAAACAGT GAAGACTTTA AGAACACAGC GGCCAGCAAT GGTACAAACA 
GAGGACCAGT ATCAACTGTG CTATAGAGCC GCACTGGAGT ATCTGGGCAG CTTTGACCAC 
TATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_031900934.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756794.1 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X15, mRNA.

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CloneID	OXa57141
Clone ID Related Accession (Same CDS sequence)	XM_031900934.1
Accession Version	XM_031900934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTGAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACACT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGACATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ATGCCGAGTG CACCACCAAG AAAGGTTGAA GTAGACTCTG TAAACTCCAC ATCTGTTCGT 
GTGACATGGA AGTCTCCTCT ACCAACCAAG CAAAATGGGC AAATTAGGGG GTATCAAGTC 
ACTTATGTTC GTCTAGAAGG TGGAGAGCCT CGGGGGAATC CCATCATAAA AGATGTTATG 
CTGTCAGAGG CTCAAGATGC TGTTATCACT GGCCTCTCTC CTGAGACCAC TTATTCCATC 
ACTGTGGCCG CCTACACTAC TAAGGGAGAT GGTGCTAGAA GCAAACCCAA GGTCGTTAGC 
ACCACAGGAG CTGTGCCTGG TAAACCCACT ATGATGATCA GCACCACAGC ACTGAACACT 
GCTCTGATCC AGTGGCATCC TCCAAGAGAG ATTGTGGGAG AGTTGTTAGG ATATCGTTTG 
CAGTACAAGC GAGTGGATGA AGACAGGTTT ATTACAATTG ATTTTAGCAA GAATGAACAT 
CACCACACAG TGACTGGGCT ACATAAAGGT GCAAGCTATA TATTCAAGCT TTCTGCAAAA 
AACCGAGCTG GAGCAGGGGA GGAATATGAA AAGGAAATTT CCACAGCTGA AGATTTTCCT 
AGTGGGTTTC CCCAAAATCT GCAAGTAGTT GGACTTACAA CCTCTACTAC AGAAGTGTCC 
TGGGAACCAC CAGTGTTGTC TGAGCGCAAT GGAAAGATCA CCAACTACAC AGTGGTGTAT 
CGGGACATAA ATAGCCAGCA GGATCTCACC AATGTCACTC GCGACACACA CATAACATTG 
GTCAATCTCA AGCCAGACAC CACATATGAC ATCAAGGTCA GGGCAAGAAC AAACAAAGGC 
GCTGGCCCTC TAAGTCCTAG CATTCAATCA CGTACAATGC CTGTTGACCA AGTGTTTGCT 
AAGAATTTCA GAGTAAACGC CGTGACAAAA ACCTCAGTCT TGCTAAGCTG GGAGCTGCCT 
GATTCTTACA AATCAGCTGT GCCTTTCAAG ATCTTTTACA ATAATCAACA TGTAGAAGTT 
GATGGACACT CTATGCGCAA ACTGATCAGT AATCTCAAGC CAGATACAGA ATACTCCTTT 
GTGCTGATGA ACAGGGGGAG CAGTGCAGGT GGACTGCAGC ACCGCGTGTC CATTCGCACA 
GCACCAGATG TTTTAAAAAG CAAGCCTTTT GCTGCCAACA AATACATGGA GGAGGGCAGG 
TTCACCTTGC TGTTGCCTCA GGTTCAGACT ACTTCCCCAG TAAGGTGGTA TTATATTGTG 
GTTGTCCCAC TGGACAGTTC CAGCGGGGGT CTGAATTCTC GCTGGCAGAG CCCTGAGGAA 
ATGGAGCTAG AGCAGATACT TGAAGCAATA AACCAGGGAT CCCGTCGTCA TAGAAGACAG 
GCAAGCAAGA ATAAGCCATA CATTGCAGCC CGAGTAAACA CACTTCCAGA ATCCTTTATT 
TTGGGTGACA ATAAAGAATA TAATGGGTTC ATCAACAAGC CACTGAATCA GAATTTACGC 
TACACCTGCT TTGTGCTAGC TGCACTAGAA GATGGGGACA CTAAAAAGTA TGCAGCCAGC 
CCATACTCAG ATGATATTGT GGTGGAGGTG ATCTCACAGG CAAAACAACA GGAAGAGCCA 
GAAATGCTTT GGGTCATGGG TCCTGTTCTG GCTGTAATTC TTATAATAAT TATAGTTATT 
GCCATTCTAC TTTTCAAAAG GAAAAGGACC AACTCTCCTT CAGGCAAAGA TGAACAGTCA 
GTAGGACTTA AAGACTGTCT CCTCGCTCAC TCCTCAGATC CAGTTGAAAT GAGGCGTCTA 
AACTACCAAA CTCCAGGTAT GAGAGACCAC CCTCCTATTC CAATCTCTGA CCTGGCAGAC 
AACATTGAAA GACTGAAGGC TAATGATGGA CTCAAGTTCT CTCAGGAATA TGAGTCTATT 
GATCCAGGTC AGCAATTCAC GTGGGAAAAT TCAAATCTAG AAGTAAACAA ACCAAAGAAT 
CGCTATGCAA ATGTCATTGC ATATGACCAT TCAAGGGTCA TTCTTACCTC AGTGGATGGA 
GTTCCCGGAA GTGATTACAT TAATGCCAAT TACATAGATG GGTACAGGAA ACAGAATGCC 
TATATAGCTA CGCAGGGCCC ACTGCCAGAG ACTATCACTG ATTTCTGGAG AATGGTGTGG 
GAGCAAAGAA CCGCCACTAT TGTCATGATG ACCCGGCTAG AGGAAAAATC AAGGGTGAAA 
TGTGACCAGT ACTGGCCCGG TCGTGGCTCA GAGACCTATG GTATGATCCA AGTCACATTA 
ATGGACACAG TAGAGCTTGC CACCTACACA GTTAGGACTT TCACACTTTA TAAGAATGGC 
TCCAGTGAGA AGCGGGATGT CAGACAGTTC CAATTTATGG CCTGGCCTGA TCACGGGGTC 
CCTGAATATC CTACTCCTAT TCTTGGATTC CTTCGCAGAG TGAAAGGCTG TAATCCACCT 
GATGCTGGTC CTATGGTTGT TCATTGCAGT GCTGGCGTGG GCAGAACAGG ATGCTTTATT 
GTTATTGATG CCATGCTAGA ACGGGTGAAA CTGGAGAAAA CTGTGGATAT TTATGGTCAT 
GTGACCTGCA TGCGCTCCCA ACGAAATTAT ATGGTGCAAA CAGAGGACCA GTACATCTTT 
ATCCATGAGG CTTTGCTTGA AGCAGTAATG TGCGGGATCA CTGAAGTGCC AGCACGCAGT 
CTGTATGCAC ATATCCAGAA ACTGTCTCAG GTCCCTCCGG GTGAGAGTGT TACCTCCATG 
GAACTAGAGT TCAAGCTTTT GGCCAACTCT AAAGCCCACA CCTCACGATT TATCAGTGCC 
AACCTCCCAT GCAACAAGTT CAAAAACAGA CTGGTAAACA TCATGCCATA TGAACTGAGT 
CGTGTCTCCC TGCAGCCCAT TCGTGGTGTT GAGGGATCAG ATTATATCAA TGCAAGTTTC 
ATTGATGGCT ACAGGCAGCA GAAAGGCTAC ATTGCTACTC AGGGTCCATT GGCCGAGACC 
ACTGAGGATT TCTGGAGAAT GCTGTGGGAA CATAACTCCA CCATTGTGGT GATGCTGACA 
AAGTTGCGAG AAATGGGGAG GGAGAAGTGC CATCAGTACT GGCCTGCAGA GCGCTCAGCA 
CGGTACCAGT ATTTTGTGGT GGACCCTATG GCTGAGTATA ACATGCCTCA GTATATCCTG 
CGTGAGTTCA AAGTGACAGA TGCACGGGAT GGCCAATCTC GTACAATTAG ACAATTCCAG 
TTCACTGATT GGCCTGAACA AGGAGTGCCA AAAACAGGTG AAGGCTTTAT TGACTTCATT 
GGGCAGGTGC ACAAAACCAA GGAGCAGTTT GGGCAGGATG GGCCAATCAC CGTTCACTGC 
AGCGCTGGCG TGGGCCGCAC TGGAGTATTC ATCACTCTCA GTATTGTACT GGAGCGTATG 
AGGTACGAGG GTGTGGTTGA CATGTTTCAA ACAGTGAAGA CTTTAAGAAC ACAGCGGCCA 
GCAATGGTAC AAACAGAGGA CCAGTATCAA CTGTGCTATA GAGCCGCACT GGAGTATCTG 
GGCAGCTTTG ACCACTATGC AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756794.1
CDS	636..6002
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSMPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKRKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_031900934.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900934.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTGAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACACT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGACATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ATGCCGAGTG CACCACCAAG AAAGGTTGAA GTAGACTCTG TAAACTCCAC ATCTGTTCGT 
GTGACATGGA AGTCTCCTCT ACCAACCAAG CAAAATGGGC AAATTAGGGG GTATCAAGTC 
ACTTATGTTC GTCTAGAAGG TGGAGAGCCT CGGGGGAATC CCATCATAAA AGATGTTATG 
CTGTCAGAGG CTCAAGATGC TGTTATCACT GGCCTCTCTC CTGAGACCAC TTATTCCATC 
ACTGTGGCCG CCTACACTAC TAAGGGAGAT GGTGCTAGAA GCAAACCCAA GGTCGTTAGC 
ACCACAGGAG CTGTGCCTGG TAAACCCACT ATGATGATCA GCACCACAGC ACTGAACACT 
GCTCTGATCC AGTGGCATCC TCCAAGAGAG ATTGTGGGAG AGTTGTTAGG ATATCGTTTG 
CAGTACAAGC GAGTGGATGA AGACAGGTTT ATTACAATTG ATTTTAGCAA GAATGAACAT 
CACCACACAG TGACTGGGCT ACATAAAGGT GCAAGCTATA TATTCAAGCT TTCTGCAAAA 
AACCGAGCTG GAGCAGGGGA GGAATATGAA AAGGAAATTT CCACAGCTGA AGATTTTCCT 
AGTGGGTTTC CCCAAAATCT GCAAGTAGTT GGACTTACAA CCTCTACTAC AGAAGTGTCC 
TGGGAACCAC CAGTGTTGTC TGAGCGCAAT GGAAAGATCA CCAACTACAC AGTGGTGTAT 
CGGGACATAA ATAGCCAGCA GGATCTCACC AATGTCACTC GCGACACACA CATAACATTG 
GTCAATCTCA AGCCAGACAC CACATATGAC ATCAAGGTCA GGGCAAGAAC AAACAAAGGC 
GCTGGCCCTC TAAGTCCTAG CATTCAATCA CGTACAATGC CTGTTGACCA AGTGTTTGCT 
AAGAATTTCA GAGTAAACGC CGTGACAAAA ACCTCAGTCT TGCTAAGCTG GGAGCTGCCT 
GATTCTTACA AATCAGCTGT GCCTTTCAAG ATCTTTTACA ATAATCAACA TGTAGAAGTT 
GATGGACACT CTATGCGCAA ACTGATCAGT AATCTCAAGC CAGATACAGA ATACTCCTTT 
GTGCTGATGA ACAGGGGGAG CAGTGCAGGT GGACTGCAGC ACCGCGTGTC CATTCGCACA 
GCACCAGATG TTTTAAAAAG CAAGCCTTTT GCTGCCAACA AATACATGGA GGAGGGCAGG 
TTCACCTTGC TGTTGCCTCA GGTTCAGACT ACTTCCCCAG TAAGGTGGTA TTATATTGTG 
GTTGTCCCAC TGGACAGTTC CAGCGGGGGT CTGAATTCTC GCTGGCAGAG CCCTGAGGAA 
ATGGAGCTAG AGCAGATACT TGAAGCAATA AACCAGGGAT CCCGTCGTCA TAGAAGACAG 
GCAAGCAAGA ATAAGCCATA CATTGCAGCC CGAGTAAACA CACTTCCAGA ATCCTTTATT 
TTGGGTGACA ATAAAGAATA TAATGGGTTC ATCAACAAGC CACTGAATCA GAATTTACGC 
TACACCTGCT TTGTGCTAGC TGCACTAGAA GATGGGGACA CTAAAAAGTA TGCAGCCAGC 
CCATACTCAG ATGATATTGT GGTGGAGGTG ATCTCACAGG CAAAACAACA GGAAGAGCCA 
GAAATGCTTT GGGTCATGGG TCCTGTTCTG GCTGTAATTC TTATAATAAT TATAGTTATT 
GCCATTCTAC TTTTCAAAAG GAAAAGGACC AACTCTCCTT CAGGCAAAGA TGAACAGTCA 
GTAGGACTTA AAGACTGTCT CCTCGCTCAC TCCTCAGATC CAGTTGAAAT GAGGCGTCTA 
AACTACCAAA CTCCAGGTAT GAGAGACCAC CCTCCTATTC CAATCTCTGA CCTGGCAGAC 
AACATTGAAA GACTGAAGGC TAATGATGGA CTCAAGTTCT CTCAGGAATA TGAGTCTATT 
GATCCAGGTC AGCAATTCAC GTGGGAAAAT TCAAATCTAG AAGTAAACAA ACCAAAGAAT 
CGCTATGCAA ATGTCATTGC ATATGACCAT TCAAGGGTCA TTCTTACCTC AGTGGATGGA 
GTTCCCGGAA GTGATTACAT TAATGCCAAT TACATAGATG GGTACAGGAA ACAGAATGCC 
TATATAGCTA CGCAGGGCCC ACTGCCAGAG ACTATCACTG ATTTCTGGAG AATGGTGTGG 
GAGCAAAGAA CCGCCACTAT TGTCATGATG ACCCGGCTAG AGGAAAAATC AAGGGTGAAA 
TGTGACCAGT ACTGGCCCGG TCGTGGCTCA GAGACCTATG GTATGATCCA AGTCACATTA 
ATGGACACAG TAGAGCTTGC CACCTACACA GTTAGGACTT TCACACTTTA TAAGAATGGC 
TCCAGTGAGA AGCGGGATGT CAGACAGTTC CAATTTATGG CCTGGCCTGA TCACGGGGTC 
CCTGAATATC CTACTCCTAT TCTTGGATTC CTTCGCAGAG TGAAAGGCTG TAATCCACCT 
GATGCTGGTC CTATGGTTGT TCATTGCAGT GCTGGCGTGG GCAGAACAGG ATGCTTTATT 
GTTATTGATG CCATGCTAGA ACGGGTGAAA CTGGAGAAAA CTGTGGATAT TTATGGTCAT 
GTGACCTGCA TGCGCTCCCA ACGAAATTAT ATGGTGCAAA CAGAGGACCA GTACATCTTT 
ATCCATGAGG CTTTGCTTGA AGCAGTAATG TGCGGGATCA CTGAAGTGCC AGCACGCAGT 
CTGTATGCAC ATATCCAGAA ACTGTCTCAG GTCCCTCCGG GTGAGAGTGT TACCTCCATG 
GAACTAGAGT TCAAGCTTTT GGCCAACTCT AAAGCCCACA CCTCACGATT TATCAGTGCC 
AACCTCCCAT GCAACAAGTT CAAAAACAGA CTGGTAAACA TCATGCCATA TGAACTGAGT 
CGTGTCTCCC TGCAGCCCAT TCGTGGTGTT GAGGGATCAG ATTATATCAA TGCAAGTTTC 
ATTGATGGCT ACAGGCAGCA GAAAGGCTAC ATTGCTACTC AGGGTCCATT GGCCGAGACC 
ACTGAGGATT TCTGGAGAAT GCTGTGGGAA CATAACTCCA CCATTGTGGT GATGCTGACA 
AAGTTGCGAG AAATGGGGAG GGAGAAGTGC CATCAGTACT GGCCTGCAGA GCGCTCAGCA 
CGGTACCAGT ATTTTGTGGT GGACCCTATG GCTGAGTATA ACATGCCTCA GTATATCCTG 
CGTGAGTTCA AAGTGACAGA TGCACGGGAT GGCCAATCTC GTACAATTAG ACAATTCCAG 
TTCACTGATT GGCCTGAACA AGGAGTGCCA AAAACAGGTG AAGGCTTTAT TGACTTCATT 
GGGCAGGTGC ACAAAACCAA GGAGCAGTTT GGGCAGGATG GGCCAATCAC CGTTCACTGC 
AGCGCTGGCG TGGGCCGCAC TGGAGTATTC ATCACTCTCA GTATTGTACT GGAGCGTATG 
AGGTACGAGG GTGTGGTTGA CATGTTTCAA ACAGTGAAGA CTTTAAGAAC ACAGCGGCCA 
GCAATGGTAC AAACAGAGGA CCAGTATCAA CTGTGCTATA GAGCCGCACT GGAGTATCTG 
GGCAGCTTTG ACCACTATGC AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_031900935.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756795.1 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X16, mRNA.

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CloneID	OXa57142
Clone ID Related Accession (Same CDS sequence)	XM_031900935.1
Accession Version	XM_031900935.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAT GCCGAGTGCA CCACCAAGAA AGGTTGAAGT AGACTCTGTA 
AACTCCACAT CTGTTCGTGT GACATGGAAG TCTCCTCTAC CAACCAAGCA AAATGGGCAA 
ATTAGGGGGT ATCAAGTCAC TTATGTTCGT CTAGAAGGTG GAGAGCCTCG GGGGAATCCC 
ATCATAAAAG ATGTTATGCT GTCAGAGGCT CAATGGAGAC CTGAGGATGT GGGAGATTAC 
GATGCTGTTA TCACTGGCCT CTCTCCTGAG ACCACTTATT CCATCACTGT GGCCGCCTAC 
ACTACTAAGG GAGATGGTGC TAGAAGCAAA CCCAAGGTCG TTAGCACCAC AGGAGCTGTG 
CCTGGTAAAC CCACTATGAT GATCAGCACC ACAGCACTGA ACACTGCTCT GATCCAGTGG 
CATCCTCCAA GAGAGATTGT GGGAGAGTTG TTAGGATATC GTTTGCAGTA CAAGCGAGTG 
GATGAAGACA GGTTTATTAC AATTGATTTT AGCAAGAATG AACATCACCA CACAGTGACT 
GGGCTACATA AAGGTGCAAG CTATATATTC AAGCTTTCTG CAAAAAACCG AGCTGGAGCA 
GGGGAGGAAT ATGAAAAGGA AATTTCCACA GCTGAAGATT TTCCTAGTGG GTTTCCCCAA 
AATCTGCAAG TAGTTGGACT TACAACCTCT ACTACAGAAG TGTCCTGGGA ACCACCAGTG 
TTGTCTGAGC GCAATGGAAA GATCACCAAC TACACAGTGG TGTATCGGGA CATAAATAGC 
CAGCAGGATC TCACCAATGT CACTCGCGAC ACACACATAA CATTGGTCAA TCTCAAGCCA 
GACACCACAT ATGACATCAA GGTCAGGGCA AGAACAAACA AAGGCGCTGG CCCTCTAAGT 
CCTAGCATTC AATCACGTAC AATGCCTGTT GACCAAGTGT TTGCTAAGAA TTTCAGAGTA 
AACGCCGTGA CAAAAACCTC AGTCTTGCTA AGCTGGGAGC TGCCTGATTC TTACAAATCA 
GCTGTGCCTT TCAAGATCTT TTACAATAAT CAACATGTAG AAGTTGATGG ACACTCTATG 
CGCAAACTGA TCAGTAATCT CAAGCCAGAT ACAGAATACT CCTTTGTGCT GATGAACAGG 
GGGAGCAGTG CAGGTGGACT GCAGCACCGC GTGTCCATTC GCACAGCACC AGATGTTTTA 
AAAAGCAAGC CTTTTGCTGC CAACAAATAC ATGGAGGAGG GCAGGTTCAC CTTGCTGTTG 
CCTCAGGTTC AGACTACTTC CCCAGTAAGG TGGTATTATA TTGTGGTTGT CCCACTGGAC 
AGTTCCAGCG GGGGTCTGAA TTCTCGCTGG CAGAGCCCTG AGGAAATGGA GCTAGAGCAG 
ATACTTGAAG CAATAAACCA GGGATCCCGT CGTCATAGAA GACAGGCAAG CAAGAATAAG 
CCATACATTG CAGCCCGAGT AAACACACTT CCAGAATCCT TTATTTTGGG TGACAATAAA 
GAATATAATG GGTTCATCAA CAAGCCACTG AATCAGAATT TACGCTACAC CTGCTTTGTG 
CTAGCTGCAC TAGAAGATGG GGACACTAAA AAGTATGCAG CCAGCCCATA CTCAGATGAT 
ATTGTGGTGG AGGTGATCTC ACAGGCAAAA CAACAGGAAG AGCCAGAAAT GCTTTGGGTC 
ATGGGTCCTG TTCTGGCTGT AATTCTTATA ATAATTATAG TTATTGCCAT TCTACTTTTC 
AAAAGGAAAA GGACCAACTC TCCTTCAGGC AAAGATGAAC AGTCAGTAGG ACTTAAAGAC 
TGTCTCCTCG CTCACTCCTC AGATCCAGTT GAAATGAGGC GTCTAAACTA CCAAACTCCA 
GGTTCCAGTG TCCCCAGTTA CCCGAATATC TCAAGTATGA GAGACCACCC TCCTATTCCA 
ATCTCTGACC TGGCAGACAA CATTGAAAGA CTGAAGGCTA ATGATGGACT CAAGTTCTCT 
CAGGAATATG AGTCTATTGA TCCAGGTCAG CAATTCACGT GGGAAAATTC AAATCTAGAA 
GTAAACAAAC CAAAGAATCG CTATGCAAAT GTCATTGCAT ATGACCATTC AAGGGTCATT 
CTTACCTCAG TGGATGGAGT TCCCGGAAGT GATTACATTA ATGCCAATTA CATAGATGGG 
TACAGGAAAC AGAATGCCTA TATAGCTACG CAGGGCCCAC TGCCAGAGAC TATCACTGAT 
TTCTGGAGAA TGGTGTGGGA GCAAAGAACC GCCACTATTG TCATGATGAC CCGGCTAGAG 
GAAAAATCAA GGGTGAAATG TGACCAGTAC TGGCCCGGTC GTGGCTCAGA GACCTATGGT 
ATGATCCAAG TCACATTAAT GGACACAGTA GAGCTTGCCA CCTACACAGT TAGGACTTTC 
ACACTTTATA AGAATGGCTC CAGTGAGAAG CGGGATGTCA GACAGTTCCA ATTTATGGCC 
TGGCCTGATC ACGGGGTCCC TGAATATCCT ACTCCTATTC TTGGATTCCT TCGCAGAGTG 
AAAGGCTGTA ATCCACCTGA TGCTGGTCCT ATGGTTGTTC ATTGCAGTGC TGGCGTGGGC 
AGAACAGGAT GCTTTATTGT TATTGATGCC ATGCTAGAAC GGGTGAAACT GGAGAAAACT 
GTGGATATTT ATGGTCATGT GACCTGCATG CGCTCCCAAC GAAATTATAT GGTGCAAACA 
GAGGACCAGT ACATCTTTAT CCATGAGGCT TTGCTTGAAG CAGTAATGTG CGGGATCACT 
GAAGTGCCAG CACGCAGTCT GTATGCACAT ATCCAGAAAC TGTCTCAGGT CCCTCCGGGT 
GAGAGTGTTA CCTCCATGGA ACTAGAGTTC AAGCTTTTGG CCAACTCTAA AGCCCACACC 
TCACGATTTA TCAGTGCCAA CCTCCCATGC AACAAGTTCA AAAACAGACT GGTAAACATC 
ATGCCATATG AACTGAGTCG TGTCTCCCTG CAGCCCATTC GTGGTGTTGA GGGATCAGAT 
TATATCAATG CAAGTTTCAT TGATGGCTAC AGGCAGCAGA AAGGCTACAT TGCTACTCAG 
GGTCCATTGG CCGAGACCAC TGAGGATTTC TGGAGAATGC TGTGGGAACA TAACTCCACC 
ATTGTGGTGA TGCTGACAAA GTTGCGAGAA ATGGGGAGGG AGAAGTGCCA TCAGTACTGG 
CCTGCAGAGC GCTCAGCACG GTACCAGTAT TTTGTGGTGG ACCCTATGGC TGAGTATAAC 
ATGCCTCAGT ATATCCTGCG TGAGTTCAAA GTGACAGATG CACGGGATGG CCAATCTCGT 
ACAATTAGAC AATTCCAGTT CACTGATTGG CCTGAACAAG GAGTGCCAAA AACAGGTGAA 
GGCTTTATTG ACTTCATTGG GCAGGTGCAC AAAACCAAGG AGCAGTTTGG GCAGGATGGG 
CCAATCACCG TTCACTGCAG CGCTGGCGTG GGCCGCACTG GAGTATTCAT CACTCTCAGT 
ATTGTACTGG AGCGTATGAG GTACGAGGGT GTGGTTGACA TGTTTCAAAC AGTGAAGACT 
TTAAGAACAC AGCGGCCAGC AATGGTACAA ACAGAGGACC AGTATCAACT GTGCTATAGA 
GCCGCACTGG AGTATCTGGG CAGCTTTGAC CACTATGCAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756795.1
CDS	636..6080
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGGTPIRGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSMPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQWRPEDVGDYDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKRKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGSSVPSYPNISSMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_031900935.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900935.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGGTACACCA ATCAGAGGTG CCTTGCAGAT AGAAAACAGC 
GAGGAATCTG ATCAGGGCAA ATATGAATGT GTTGCAACCA ACAGTGCAGG GACACGTTAC 
TCAGCTCCTG CTAACCTCTA TGTTCGTGTC CGTCGAGTGG CACCAAGATT TTCTATCCCT 
CCCAGTAACC ATGAAGTGAT GCCTGGGGGC AGTGTTAATT TAACATGTGT AGCAGTGGGG 
GCCCCTATGC CCTATGTCAA ATGGATGGCT GGCCTTGAGG AACTCACCAA AGAAGATGAA 
ATGCCTGTGG GCCGCAATGT TTTAGAACTG ACCAATATCA AAGAGTCTGC CAATTACACC 
TGTGTTGCGA TCTCCTCATT AGGCATGATT GAAGCAGTTG CACAGATCAC TGTGAAAGCA 
TTGCCAAAAC CGCCTTTGGA TGGTACAGTA ACAGAGACTA CAGCCACTAG TGTCACCCTA 
ACTTGGGATT CTGGCAACCC AGACCCAGTC TCTTATTATG TGATTCAGTA TAAACCCAAA 
GCTTCAGAAA GCTCATTTCA AGAAGTTGAT GGAGTGGCAA CAACTCGGTA CAGTATTGGG 
GGACTGAGCC CCTTCTCAGA GTATGAATTT CGCATCATTG CCGTAAACAA TATTGGCCGA 
GGACCTCCAA GTGAAGTGAT AGAGGCTCAG ACAGGGGAAC AGGCACCCTC AAGCCCACCT 
TTGAAGGTCC AAGCTCGTAT GTTAAGTGCC AGTACTATGC TAGTTCAGTG GGACCTACCA 
GAAGAACCAA ATGGCCAGAT AAGAGGTTTC CGGGTTTATT ACACTACTGA TCCACACTTG 
CCTTTTAGTA TGTGGCAAAA ACACAATGTT GTTGACAACC ACTTGACAAC AATAGGAGGT 
CTGACCACAG GAACCACTTA CAGCATTCGT GTTCTTGCCT TCACATCTGT CGGGGATGGA 
CCGCCTTCTG ACATAATTCA GGTCAAAACA CAGCAGGGAG TTCCTGCGCA GCCTGTTGAT 
TTCCAGGCAG AAGCTGAGAC TGACACACGC ATATTGCTCA CGTGGCTTCT CCCACCACAA 
GAACGCATTA CCAAATATGA GCTGCTTTAC TGGGAAGGAA CAGATGGAGA ACAGCAAAAG 
CTTATGTTTC ACCCTACCTC CTCTTATGCT GTGGAGGGTC TGAAACCGGA CACACTTTAT 
CATTTCCGAT TAGCTGCGTG GTCTGACACT GGAGTTGGTG TTTACACCCC AGCAATTCAA 
GCTAGAACTG CACAATCTAT GCCGAGTGCA CCACCAAGAA AGGTTGAAGT AGACTCTGTA 
AACTCCACAT CTGTTCGTGT GACATGGAAG TCTCCTCTAC CAACCAAGCA AAATGGGCAA 
ATTAGGGGGT ATCAAGTCAC TTATGTTCGT CTAGAAGGTG GAGAGCCTCG GGGGAATCCC 
ATCATAAAAG ATGTTATGCT GTCAGAGGCT CAATGGAGAC CTGAGGATGT GGGAGATTAC 
GATGCTGTTA TCACTGGCCT CTCTCCTGAG ACCACTTATT CCATCACTGT GGCCGCCTAC 
ACTACTAAGG GAGATGGTGC TAGAAGCAAA CCCAAGGTCG TTAGCACCAC AGGAGCTGTG 
CCTGGTAAAC CCACTATGAT GATCAGCACC ACAGCACTGA ACACTGCTCT GATCCAGTGG 
CATCCTCCAA GAGAGATTGT GGGAGAGTTG TTAGGATATC GTTTGCAGTA CAAGCGAGTG 
GATGAAGACA GGTTTATTAC AATTGATTTT AGCAAGAATG AACATCACCA CACAGTGACT 
GGGCTACATA AAGGTGCAAG CTATATATTC AAGCTTTCTG CAAAAAACCG AGCTGGAGCA 
GGGGAGGAAT ATGAAAAGGA AATTTCCACA GCTGAAGATT TTCCTAGTGG GTTTCCCCAA 
AATCTGCAAG TAGTTGGACT TACAACCTCT ACTACAGAAG TGTCCTGGGA ACCACCAGTG 
TTGTCTGAGC GCAATGGAAA GATCACCAAC TACACAGTGG TGTATCGGGA CATAAATAGC 
CAGCAGGATC TCACCAATGT CACTCGCGAC ACACACATAA CATTGGTCAA TCTCAAGCCA 
GACACCACAT ATGACATCAA GGTCAGGGCA AGAACAAACA AAGGCGCTGG CCCTCTAAGT 
CCTAGCATTC AATCACGTAC AATGCCTGTT GACCAAGTGT TTGCTAAGAA TTTCAGAGTA 
AACGCCGTGA CAAAAACCTC AGTCTTGCTA AGCTGGGAGC TGCCTGATTC TTACAAATCA 
GCTGTGCCTT TCAAGATCTT TTACAATAAT CAACATGTAG AAGTTGATGG ACACTCTATG 
CGCAAACTGA TCAGTAATCT CAAGCCAGAT ACAGAATACT CCTTTGTGCT GATGAACAGG 
GGGAGCAGTG CAGGTGGACT GCAGCACCGC GTGTCCATTC GCACAGCACC AGATGTTTTA 
AAAAGCAAGC CTTTTGCTGC CAACAAATAC ATGGAGGAGG GCAGGTTCAC CTTGCTGTTG 
CCTCAGGTTC AGACTACTTC CCCAGTAAGG TGGTATTATA TTGTGGTTGT CCCACTGGAC 
AGTTCCAGCG GGGGTCTGAA TTCTCGCTGG CAGAGCCCTG AGGAAATGGA GCTAGAGCAG 
ATACTTGAAG CAATAAACCA GGGATCCCGT CGTCATAGAA GACAGGCAAG CAAGAATAAG 
CCATACATTG CAGCCCGAGT AAACACACTT CCAGAATCCT TTATTTTGGG TGACAATAAA 
GAATATAATG GGTTCATCAA CAAGCCACTG AATCAGAATT TACGCTACAC CTGCTTTGTG 
CTAGCTGCAC TAGAAGATGG GGACACTAAA AAGTATGCAG CCAGCCCATA CTCAGATGAT 
ATTGTGGTGG AGGTGATCTC ACAGGCAAAA CAACAGGAAG AGCCAGAAAT GCTTTGGGTC 
ATGGGTCCTG TTCTGGCTGT AATTCTTATA ATAATTATAG TTATTGCCAT TCTACTTTTC 
AAAAGGAAAA GGACCAACTC TCCTTCAGGC AAAGATGAAC AGTCAGTAGG ACTTAAAGAC 
TGTCTCCTCG CTCACTCCTC AGATCCAGTT GAAATGAGGC GTCTAAACTA CCAAACTCCA 
GGTTCCAGTG TCCCCAGTTA CCCGAATATC TCAAGTATGA GAGACCACCC TCCTATTCCA 
ATCTCTGACC TGGCAGACAA CATTGAAAGA CTGAAGGCTA ATGATGGACT CAAGTTCTCT 
CAGGAATATG AGTCTATTGA TCCAGGTCAG CAATTCACGT GGGAAAATTC AAATCTAGAA 
GTAAACAAAC CAAAGAATCG CTATGCAAAT GTCATTGCAT ATGACCATTC AAGGGTCATT 
CTTACCTCAG TGGATGGAGT TCCCGGAAGT GATTACATTA ATGCCAATTA CATAGATGGG 
TACAGGAAAC AGAATGCCTA TATAGCTACG CAGGGCCCAC TGCCAGAGAC TATCACTGAT 
TTCTGGAGAA TGGTGTGGGA GCAAAGAACC GCCACTATTG TCATGATGAC CCGGCTAGAG 
GAAAAATCAA GGGTGAAATG TGACCAGTAC TGGCCCGGTC GTGGCTCAGA GACCTATGGT 
ATGATCCAAG TCACATTAAT GGACACAGTA GAGCTTGCCA CCTACACAGT TAGGACTTTC 
ACACTTTATA AGAATGGCTC CAGTGAGAAG CGGGATGTCA GACAGTTCCA ATTTATGGCC 
TGGCCTGATC ACGGGGTCCC TGAATATCCT ACTCCTATTC TTGGATTCCT TCGCAGAGTG 
AAAGGCTGTA ATCCACCTGA TGCTGGTCCT ATGGTTGTTC ATTGCAGTGC TGGCGTGGGC 
AGAACAGGAT GCTTTATTGT TATTGATGCC ATGCTAGAAC GGGTGAAACT GGAGAAAACT 
GTGGATATTT ATGGTCATGT GACCTGCATG CGCTCCCAAC GAAATTATAT GGTGCAAACA 
GAGGACCAGT ACATCTTTAT CCATGAGGCT TTGCTTGAAG CAGTAATGTG CGGGATCACT 
GAAGTGCCAG CACGCAGTCT GTATGCACAT ATCCAGAAAC TGTCTCAGGT CCCTCCGGGT 
GAGAGTGTTA CCTCCATGGA ACTAGAGTTC AAGCTTTTGG CCAACTCTAA AGCCCACACC 
TCACGATTTA TCAGTGCCAA CCTCCCATGC AACAAGTTCA AAAACAGACT GGTAAACATC 
ATGCCATATG AACTGAGTCG TGTCTCCCTG CAGCCCATTC GTGGTGTTGA GGGATCAGAT 
TATATCAATG CAAGTTTCAT TGATGGCTAC AGGCAGCAGA AAGGCTACAT TGCTACTCAG 
GGTCCATTGG CCGAGACCAC TGAGGATTTC TGGAGAATGC TGTGGGAACA TAACTCCACC 
ATTGTGGTGA TGCTGACAAA GTTGCGAGAA ATGGGGAGGG AGAAGTGCCA TCAGTACTGG 
CCTGCAGAGC GCTCAGCACG GTACCAGTAT TTTGTGGTGG ACCCTATGGC TGAGTATAAC 
ATGCCTCAGT ATATCCTGCG TGAGTTCAAA GTGACAGATG CACGGGATGG CCAATCTCGT 
ACAATTAGAC AATTCCAGTT CACTGATTGG CCTGAACAAG GAGTGCCAAA AACAGGTGAA 
GGCTTTATTG ACTTCATTGG GCAGGTGCAC AAAACCAAGG AGCAGTTTGG GCAGGATGGG 
CCAATCACCG TTCACTGCAG CGCTGGCGTG GGCCGCACTG GAGTATTCAT CACTCTCAGT 
ATTGTACTGG AGCGTATGAG GTACGAGGGT GTGGTTGACA TGTTTCAAAC AGTGAAGACT 
TTAAGAACAC AGCGGCCAGC AATGGTACAA ACAGAGGACC AGTATCAACT GTGCTATAGA 
GCCGCACTGG AGTATCTGGG CAGCTTTGAC CACTATGCAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_031900936.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756796.1 Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X17, mRNA.

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CloneID	OXa57143
Clone ID Related Accession (Same CDS sequence)	XM_031900936.1
Accession Version	XM_031900936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTGAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACACT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGACATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ATGCCGAGTG CACCACCAAG AAAGGTTGAA GTAGACTCTG TAAACTCCAC ATCTGTTCGT 
GTGACATGGA AGTCTCCTCT ACCAACCAAG CAAAATGGGC AAATTAGGGG GTATCAAGTC 
ACTTATGTTC GTCTAGAAGG TGGAGAGCCT CGGGGGAATC CCATCATAAA AGATGTTATG 
CTGTCAGAGG CTCAATGGAG ACCTGAGGAT GTGGGAGATT ACGATGCTGT TATCACTGGC 
CTCTCTCCTG AGACCACTTA TTCCATCACT GTGGCCGCCT ACACTACTAA GGGAGATGGT 
GCTAGAAGCA AACCCAAGGT CGTTAGCACC ACAGGAGCTG TGCCTGGTAA ACCCACTATG 
ATGATCAGCA CCACAGCACT GAACACTGCT CTGATCCAGT GGCATCCTCC AAGAGAGATT 
GTGGGAGAGT TGTTAGGATA TCGTTTGCAG TACAAGCGAG TGGATGAAGA CAGGTTTATT 
ACAATTGATT TTAGCAAGAA TGAACATCAC CACACAGTGA CTGGGCTACA TAAAGGTGCA 
AGCTATATAT TCAAGCTTTC TGCAAAAAAC CGAGCTGGAG CAGGGGAGGA ATATGAAAAG 
GAAATTTCCA CAGCTGAAGA TTTTCCTAGT GGGTTTCCCC AAAATCTGCA AGTAGTTGGA 
CTTACAACCT CTACTACAGA AGTGTCCTGG GAACCACCAG TGTTGTCTGA GCGCAATGGA 
AAGATCACCA ACTACACAGT GGTGTATCGG GACATAAATA GCCAGCAGGA TCTCACCAAT 
GTCACTCGCG ACACACACAT AACATTGGTC AATCTCAAGC CAGACACCAC ATATGACATC 
AAGGTCAGGG CAAGAACAAA CAAAGGCGCT GGCCCTCTAA GTCCTAGCAT TCAATCACGT 
ACAATGCCTG TTGACCAAGT GTTTGCTAAG AATTTCAGAG TAAACGCCGT GACAAAAACC 
TCAGTCTTGC TAAGCTGGGA GCTGCCTGAT TCTTACAAAT CAGCTGTGCC TTTCAAGATC 
TTTTACAATA ATCAACATGT AGAAGTTGAT GGACACTCTA TGCGCAAACT GATCAGTAAT 
CTCAAGCCAG ATACAGAATA CTCCTTTGTG CTGATGAACA GGGGGAGCAG TGCAGGTGGA 
CTGCAGCACC GCGTGTCCAT TCGCACAGCA CCAGATGTTT TAAAAAGCAA GCCTTTTGCT 
GCCAACAAAT ACATGGAGGA GGGCAGGTTC ACCTTGCTGT TGCCTCAGGT TCAGACTACT 
TCCCCAGTAA GGTGGTATTA TATTGTGGTT GTCCCACTGG ACAGTTCCAG CGGGGGTCTG 
AATTCTCGCT GGCAGAGCCC TGAGGAAATG GAGCTAGAGC AGATACTTGA AGCAATAAAC 
CAGGGATCCC GTCGTCATAG AAGACAGGCA AGCAAGAATA AGCCATACAT TGCAGCCCGA 
GTAAACACAC TTCCAGAATC CTTTATTTTG GGTGACAATA AAGAATATAA TGGGTTCATC 
AACAAGCCAC TGAATCAGAA TTTACGCTAC ACCTGCTTTG TGCTAGCTGC ACTAGAAGAT 
GGGGACACTA AAAAGTATGC AGCCAGCCCA TACTCAGATG ATATTGTGGT GGAGGTGATC 
TCACAGGCAA AACAACAGGA AGAGCCAGAA ATGCTTTGGG TCATGGGTCC TGTTCTGGCT 
GTAATTCTTA TAATAATTAT AGTTATTGCC ATTCTACTTT TCAAAAGGAA AAGGACCAAC 
TCTCCTTCAG GCAAAGATGA ACAGTCAGTA GGACTTAAAG ACTGTCTCCT CGCTCACTCC 
TCAGATCCAG TTGAAATGAG GCGTCTAAAC TACCAAACTC CAGGTTCCAG TGTCCCCAGT 
TACCCGAATA TCTCAAGTAT GAGAGACCAC CCTCCTATTC CAATCTCTGA CCTGGCAGAC 
AACATTGAAA GACTGAAGGC TAATGATGGA CTCAAGTTCT CTCAGGAATA TGAGTCTATT 
GATCCAGGTC AGCAATTCAC GTGGGAAAAT TCAAATCTAG AAGTAAACAA ACCAAAGAAT 
CGCTATGCAA ATGTCATTGC ATATGACCAT TCAAGGGTCA TTCTTACCTC AGTGGATGGA 
GTTCCCGGAA GTGATTACAT TAATGCCAAT TACATAGATG GGTACAGGAA ACAGAATGCC 
TATATAGCTA CGCAGGGCCC ACTGCCAGAG ACTATCACTG ATTTCTGGAG AATGGTGTGG 
GAGCAAAGAA CCGCCACTAT TGTCATGATG ACCCGGCTAG AGGAAAAATC AAGGGTGAAA 
TGTGACCAGT ACTGGCCCGG TCGTGGCTCA GAGACCTATG GTATGATCCA AGTCACATTA 
ATGGACACAG TAGAGCTTGC CACCTACACA GTTAGGACTT TCACACTTTA TAAGAATGGC 
TCCAGTGAGA AGCGGGATGT CAGACAGTTC CAATTTATGG CCTGGCCTGA TCACGGGGTC 
CCTGAATATC CTACTCCTAT TCTTGGATTC CTTCGCAGAG TGAAAGGCTG TAATCCACCT 
GATGCTGGTC CTATGGTTGT TCATTGCAGT GCTGGCGTGG GCAGAACAGG ATGCTTTATT 
GTTATTGATG CCATGCTAGA ACGGGTGAAA CTGGAGAAAA CTGTGGATAT TTATGGTCAT 
GTGACCTGCA TGCGCTCCCA ACGAAATTAT ATGGTGCAAA CAGAGGACCA GTACATCTTT 
ATCCATGAGG CTTTGCTTGA AGCAGTAATG TGCGGGATCA CTGAAGTGCC AGCACGCAGT 
CTGTATGCAC ATATCCAGAA ACTGTCTCAG GTCCCTCCGG GTGAGAGTGT TACCTCCATG 
GAACTAGAGT TCAAGCTTTT GGCCAACTCT AAAGCCCACA CCTCACGATT TATCAGTGCC 
AACCTCCCAT GCAACAAGTT CAAAAACAGA CTGGTAAACA TCATGCCATA TGAACTGAGT 
CGTGTCTCCC TGCAGCCCAT TCGTGGTGTT GAGGGATCAG ATTATATCAA TGCAAGTTTC 
ATTGATGGCT ACAGGCAGCA GAAAGGCTAC ATTGCTACTC AGGGTCCATT GGCCGAGACC 
ACTGAGGATT TCTGGAGAAT GCTGTGGGAA CATAACTCCA CCATTGTGGT GATGCTGACA 
AAGTTGCGAG AAATGGGGAG GGAGAAGTGC CATCAGTACT GGCCTGCAGA GCGCTCAGCA 
CGGTACCAGT ATTTTGTGGT GGACCCTATG GCTGAGTATA ACATGCCTCA GTATATCCTG 
CGTGAGTTCA AAGTGACAGA TGCACGGGAT GGCCAATCTC GTACAATTAG ACAATTCCAG 
TTCACTGATT GGCCTGAACA AGGAGTGCCA AAAACAGGTG AAGGCTTTAT TGACTTCATT 
GGGCAGGTGC ACAAAACCAA GGAGCAGTTT GGGCAGGATG GGCCAATCAC CGTTCACTGC 
AGCGCTGGCG TGGGCCGCAC TGGAGTATTC ATCACTCTCA GTATTGTACT GGAGCGTATG 
AGGTACGAGG GTGTGGTTGA CATGTTTCAA ACAGTGAAGA CTTTAAGAAC ACAGCGGCCA 
GCAATGGTAC AAACAGAGGA CCAGTATCAA CTGTGCTATA GAGCCGCACT GGAGTATCTG 
GGCAGCTTTG ACCACTATGC AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756796.1
CDS	636..6062
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSDIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSMPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQWRPEDVGDYDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKRKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGSSVPSYPNISSMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_031900936.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase receptor type F (ptprf), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900936.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTGAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACACT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGACATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ATGCCGAGTG CACCACCAAG AAAGGTTGAA GTAGACTCTG TAAACTCCAC ATCTGTTCGT 
GTGACATGGA AGTCTCCTCT ACCAACCAAG CAAAATGGGC AAATTAGGGG GTATCAAGTC 
ACTTATGTTC GTCTAGAAGG TGGAGAGCCT CGGGGGAATC CCATCATAAA AGATGTTATG 
CTGTCAGAGG CTCAATGGAG ACCTGAGGAT GTGGGAGATT ACGATGCTGT TATCACTGGC 
CTCTCTCCTG AGACCACTTA TTCCATCACT GTGGCCGCCT ACACTACTAA GGGAGATGGT 
GCTAGAAGCA AACCCAAGGT CGTTAGCACC ACAGGAGCTG TGCCTGGTAA ACCCACTATG 
ATGATCAGCA CCACAGCACT GAACACTGCT CTGATCCAGT GGCATCCTCC AAGAGAGATT 
GTGGGAGAGT TGTTAGGATA TCGTTTGCAG TACAAGCGAG TGGATGAAGA CAGGTTTATT 
ACAATTGATT TTAGCAAGAA TGAACATCAC CACACAGTGA CTGGGCTACA TAAAGGTGCA 
AGCTATATAT TCAAGCTTTC TGCAAAAAAC CGAGCTGGAG CAGGGGAGGA ATATGAAAAG 
GAAATTTCCA CAGCTGAAGA TTTTCCTAGT GGGTTTCCCC AAAATCTGCA AGTAGTTGGA 
CTTACAACCT CTACTACAGA AGTGTCCTGG GAACCACCAG TGTTGTCTGA GCGCAATGGA 
AAGATCACCA ACTACACAGT GGTGTATCGG GACATAAATA GCCAGCAGGA TCTCACCAAT 
GTCACTCGCG ACACACACAT AACATTGGTC AATCTCAAGC CAGACACCAC ATATGACATC 
AAGGTCAGGG CAAGAACAAA CAAAGGCGCT GGCCCTCTAA GTCCTAGCAT TCAATCACGT 
ACAATGCCTG TTGACCAAGT GTTTGCTAAG AATTTCAGAG TAAACGCCGT GACAAAAACC 
TCAGTCTTGC TAAGCTGGGA GCTGCCTGAT TCTTACAAAT CAGCTGTGCC TTTCAAGATC 
TTTTACAATA ATCAACATGT AGAAGTTGAT GGACACTCTA TGCGCAAACT GATCAGTAAT 
CTCAAGCCAG ATACAGAATA CTCCTTTGTG CTGATGAACA GGGGGAGCAG TGCAGGTGGA 
CTGCAGCACC GCGTGTCCAT TCGCACAGCA CCAGATGTTT TAAAAAGCAA GCCTTTTGCT 
GCCAACAAAT ACATGGAGGA GGGCAGGTTC ACCTTGCTGT TGCCTCAGGT TCAGACTACT 
TCCCCAGTAA GGTGGTATTA TATTGTGGTT GTCCCACTGG ACAGTTCCAG CGGGGGTCTG 
AATTCTCGCT GGCAGAGCCC TGAGGAAATG GAGCTAGAGC AGATACTTGA AGCAATAAAC 
CAGGGATCCC GTCGTCATAG AAGACAGGCA AGCAAGAATA AGCCATACAT TGCAGCCCGA 
GTAAACACAC TTCCAGAATC CTTTATTTTG GGTGACAATA AAGAATATAA TGGGTTCATC 
AACAAGCCAC TGAATCAGAA TTTACGCTAC ACCTGCTTTG TGCTAGCTGC ACTAGAAGAT 
GGGGACACTA AAAAGTATGC AGCCAGCCCA TACTCAGATG ATATTGTGGT GGAGGTGATC 
TCACAGGCAA AACAACAGGA AGAGCCAGAA ATGCTTTGGG TCATGGGTCC TGTTCTGGCT 
GTAATTCTTA TAATAATTAT AGTTATTGCC ATTCTACTTT TCAAAAGGAA AAGGACCAAC 
TCTCCTTCAG GCAAAGATGA ACAGTCAGTA GGACTTAAAG ACTGTCTCCT CGCTCACTCC 
TCAGATCCAG TTGAAATGAG GCGTCTAAAC TACCAAACTC CAGGTTCCAG TGTCCCCAGT 
TACCCGAATA TCTCAAGTAT GAGAGACCAC CCTCCTATTC CAATCTCTGA CCTGGCAGAC 
AACATTGAAA GACTGAAGGC TAATGATGGA CTCAAGTTCT CTCAGGAATA TGAGTCTATT 
GATCCAGGTC AGCAATTCAC GTGGGAAAAT TCAAATCTAG AAGTAAACAA ACCAAAGAAT 
CGCTATGCAA ATGTCATTGC ATATGACCAT TCAAGGGTCA TTCTTACCTC AGTGGATGGA 
GTTCCCGGAA GTGATTACAT TAATGCCAAT TACATAGATG GGTACAGGAA ACAGAATGCC 
TATATAGCTA CGCAGGGCCC ACTGCCAGAG ACTATCACTG ATTTCTGGAG AATGGTGTGG 
GAGCAAAGAA CCGCCACTAT TGTCATGATG ACCCGGCTAG AGGAAAAATC AAGGGTGAAA 
TGTGACCAGT ACTGGCCCGG TCGTGGCTCA GAGACCTATG GTATGATCCA AGTCACATTA 
ATGGACACAG TAGAGCTTGC CACCTACACA GTTAGGACTT TCACACTTTA TAAGAATGGC 
TCCAGTGAGA AGCGGGATGT CAGACAGTTC CAATTTATGG CCTGGCCTGA TCACGGGGTC 
CCTGAATATC CTACTCCTAT TCTTGGATTC CTTCGCAGAG TGAAAGGCTG TAATCCACCT 
GATGCTGGTC CTATGGTTGT TCATTGCAGT GCTGGCGTGG GCAGAACAGG ATGCTTTATT 
GTTATTGATG CCATGCTAGA ACGGGTGAAA CTGGAGAAAA CTGTGGATAT TTATGGTCAT 
GTGACCTGCA TGCGCTCCCA ACGAAATTAT ATGGTGCAAA CAGAGGACCA GTACATCTTT 
ATCCATGAGG CTTTGCTTGA AGCAGTAATG TGCGGGATCA CTGAAGTGCC AGCACGCAGT 
CTGTATGCAC ATATCCAGAA ACTGTCTCAG GTCCCTCCGG GTGAGAGTGT TACCTCCATG 
GAACTAGAGT TCAAGCTTTT GGCCAACTCT AAAGCCCACA CCTCACGATT TATCAGTGCC 
AACCTCCCAT GCAACAAGTT CAAAAACAGA CTGGTAAACA TCATGCCATA TGAACTGAGT 
CGTGTCTCCC TGCAGCCCAT TCGTGGTGTT GAGGGATCAG ATTATATCAA TGCAAGTTTC 
ATTGATGGCT ACAGGCAGCA GAAAGGCTAC ATTGCTACTC AGGGTCCATT GGCCGAGACC 
ACTGAGGATT TCTGGAGAAT GCTGTGGGAA CATAACTCCA CCATTGTGGT GATGCTGACA 
AAGTTGCGAG AAATGGGGAG GGAGAAGTGC CATCAGTACT GGCCTGCAGA GCGCTCAGCA 
CGGTACCAGT ATTTTGTGGT GGACCCTATG GCTGAGTATA ACATGCCTCA GTATATCCTG 
CGTGAGTTCA AAGTGACAGA TGCACGGGAT GGCCAATCTC GTACAATTAG ACAATTCCAG 
TTCACTGATT GGCCTGAACA AGGAGTGCCA AAAACAGGTG AAGGCTTTAT TGACTTCATT 
GGGCAGGTGC ACAAAACCAA GGAGCAGTTT GGGCAGGATG GGCCAATCAC CGTTCACTGC 
AGCGCTGGCG TGGGCCGCAC TGGAGTATTC ATCACTCTCA GTATTGTACT GGAGCGTATG 
AGGTACGAGG GTGTGGTTGA CATGTTTCAA ACAGTGAAGA CTTTAAGAAC ACAGCGGCCA 
GCAATGGTAC AAACAGAGGA CCAGTATCAA CTGTGCTATA GAGCCGCACT GGAGTATCTG 
GGCAGCTTTG ACCACTATGC AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_004913961.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914018.1 Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X2, mRNA.

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CloneID	OXa09448
Clone ID Related Accession (Same CDS sequence)	XM_004913961.1
Accession Version	XM_004913961.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTCAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACATT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGAGATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCAAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTAACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTGCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA ATGGAGACCT GAGGATGTGG GAGATTACGA TGCTGTTATC 
ACTGGCCTCT CTCCTGAGAC CACTTATTCC ATCACTGTGG CCGCCTACAC TACTAAGGGA 
GATGGTGCTA GAAGCAAACC CAAGGTCGTT AGCACCACAG GAGCTGTGCC TGGTAAACCC 
ACTATGATGA TCAGCACCAC AGCACTGAAC ACTGCTCTGA TCCAGTGGCA TCCTCCAAGA 
GAGATTGTGG GAGAGTTGTT AGGATATCGT TTGCAGTACA AGCGAGTGGA TGAAGACAGG 
TTTATTACAA TTGATTTTAG CAAGAATGAA CATCACCACA CAGTGACTGG GCTACATAAA 
GGTGCAAGCT ATATATTCAA GCTTTCTGCA AAAAACCGAG CTGGAGCAGG GGAGGAATAT 
GAAAAGGAAA TTTCCACAGC TGAAGATTTT CCTAGTGGGT TTCCCCAAAA TCTGCAAGTA 
GTTGGACTTA CAACCTCTAC TACAGAAGTG TCCTGGGAAC CACCAGTGTT GTCTGAGCGC 
AATGGAAAGA TCACCAACTA CACAGTGGTG TATCGGGACA TAAATAGCCA GCAGGATCTC 
ACCAATGTCA CTCGCGACAC ACACATAACA TTGGTCAATC TCAAGCCAGA CACCACATAT 
GACATCAAGG TCAGGGCAAG AACAAACAAA GGCGCTGGCC CTCTAAGTCC TAGCATTCAA 
TCACGTACAA TGCCTGTTGA CCAAGTGTTT GCTAAGAATT TCAGAGTAAA CGCCGTGACA 
AAAACCTCAG TCTTGCTAAG CTGGGAGCTG CCTGATTCTT ACAAATCAGC TGTGCCTTTC 
AAGATCTTTT ACAATAATCA ACATGTAGAA GTTGATGGAC ACTCTATGCG CAAACTGATC 
AGTAATCTCA AGCCAGATAC AGAATACTCC TTTGTGCTGA TGAACAGGGG GAGCAGTGCA 
GGTGGACTGC AGCACCGCGT GTCCATTCGC ACAGCACCAG ATGTTTTAAA AAGCAAGCCT 
TTTGCTGCCA ACAAATACAT GGAGGAGGGC AGGTTCACCT TGCTGTTGCC TCAGGTTCAG 
ACTACTTCCC CAGTAAGGTG GTATTATATT GTGGTTGTCC CACTGGACAG TTCCAGCGGG 
GGTCTGAATT CTCGCTGGCA GAGCCCTGAG GAAATGGAGC TAGAGCAGAT ACTTGAAGCA 
ATAAACCAGG GATCCCGTCG TCATAGAAGA CAGGCAAGCA AGAATAAGCC ATACATTGCA 
GCCCGAGTAA ACACACTTCC AGAATCCTTT ATTTTGGGTG ACAATAAAGA ATATAATGGG 
TTCATCAACA AGCCACTGAA TCAGAATTTA CGCTACACCT GCTTTGTGCT AGCTGCACTA 
GAAGATGGGG ACACTAAAAA GTATGCAGCC AGCCCATACT CAGATGATAT TGTGGTGGAG 
GTGATCTCAC AGGCAAAACA ACAGGAAGAG CCAGAAATGC TTTGGGTCAT GGGTCCTGTT 
CTGGCTGTAA TTCTTATAAT AATTATAGTT ATTGCCATTC TACTTTTCAA AAGGAAAAGG 
ACCAACTCTC CTTCAGGCAA AGATGAACAG TCAGTAGGAC TTAAAGACTG TCTCCTCGCT 
CACTCCTCAG ATCCAGTTGA AATGAGGCGT CTAAACTACC AAACTCCAGG TATGAGAGAC 
CACCCTCCTA TTCCAATCTC TGACCTGGCA GACAACATTG AAAGACTGAA GGCTAATGAT 
GGACTCAAGT TCTCTCAGGA ATATGAGTCT ATTGATCCAG GTCAGCAATT CACGTGGGAA 
AATTCAAATC TAGAAGTAAA CAAACCAAAG AATCGCTATG CAAATGTCAT TGCATATGAC 
CATTCAAGGG TCATTCTTAC CTCAGTGGAT GGAGTTCCCG GAAGTGATTA CATTAATGCC 
AATTACATAG ATGGGTACAG GAAACAGAAT GCCTATATAG CTACGCAGGG CCCACTGCCA 
GAGACTATCA CTGATTTCTG GAGAATGGTG TGGGAGCAAA GAACCGCCAC TATTGTCATG 
ATGACCCGGC TAGAGGAAAA ATCAAGGGTG AAATGTGACC AGTACTGGCC CGGTCGTGGC 
TCAGAGACCT ATGGTATGAT CCAAGTCACA TTAATGGACA CAGTAGAGCT TGCCACCTAC 
ACAGTTAGGA CTTTCACACT TTATAAGAAT GGCTCCAGTG AGAAGCGGGA TGTCAGACAG 
TTCCAATTTA TGGCCTGGCC TGATCACGGG GTCCCTGAAT ATCCTACTCC TATTCTTGGA 
TTCCTTCGCA GAGTGAAAGG CTGTAATCCA CCTGATGCTG GTCCTATGGT TGTTCATTGC 
AGTGCTGGTG TGGGCAGAAC AGGATGCTTT ATTGTTATTG ATGCCATGCT AGAACGGGTG 
AAACTGGAGA AAACTGTGGA TATTTATGGT CATGTGACCT GCATGCGCTC CCAACGAAAT 
TATATGGTGC AAACAGAGGA CCAGTACATC TTTATCCATG AGGCTTTGCT TGAAGCAGTA 
ATGTGCGGGA TCACTGAAGT GCCAGCACGC AGTCTGTATG CACATATCCA GAAACTGTCT 
CAGGTCCCTC CGGGTGAGAG TGTTACCTCC ATGGAACTAG AGTTCAAGCT TTTGGCCAAC 
TCTAAAGCCC ACACCTCACG ATTTATCAGT GCCAACCTCC CATGCAACAA GTTCAAAAAC 
AGACTGGTAA ACATCATGCC ATATGAACTG AGTCGTGTCT CCCTGCAGCC CATTCGTGGT 
GTTGAGGGAT CAGATTATAT CAATGCAAGT TTCATTGATG GCTACAGGCA GCAGAAAGGC 
TACATTGCTA CTCAGGGTCC ATTGGCCGAG ACCACTGAGG ATTTCTGGAG AATGCTGTGG 
GAACATAACT CCACCATTGT GGTGATGCTG ACAAAGTTGC GAGAAATGGG GAGGGAGAAG 
TGCCATCAGT ACTGGCCTGC AGAGCGCTCA GCACGGTACC AGTATTTTGT GGTGGACCCT 
ATGGCTGAGT ATAACATGCC TCAGTATATC CTGCGTGAGT TCAAAGTGAC AGATGCACGG 
GATGGCCAAT CTCGTACAAT TAGACAATTC CAGTTCACTG ATTGGCCTGA ACAAGGAGTG 
CCAAAAACAG GTGAAGGCTT TATTGACTTC ATTGGGCAGG TGCACAAAAC CAAGGAGCAG 
TTTGGGCAGG ATGGGCCAAT CACCGTTCAC TGCAGCGCTG GCGTGGGGCG CACTGGAGTA 
TTCATCACTC TCAGTATTGT ACTGGAGCGT ATGAGGTACG AGGGTGTGGT TGACATGTTT 
CAAACAGTGA AGACTTTAAG AACACAGCGG CCAGCAATGG TACAAACAGA GGACCAGTAT 
CAACTGTGTT ATAGAGCCGC ACTGGAGTAT CTGGGCAGCT TTGACCACTA TGCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914018.1
CDS	635..6334
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSEIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQWRPEDVGDYDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKRKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_004913961.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913961.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTCAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACATT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGAGATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCAAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTAACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTGCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA ATGGAGACCT GAGGATGTGG GAGATTACGA TGCTGTTATC 
ACTGGCCTCT CTCCTGAGAC CACTTATTCC ATCACTGTGG CCGCCTACAC TACTAAGGGA 
GATGGTGCTA GAAGCAAACC CAAGGTCGTT AGCACCACAG GAGCTGTGCC TGGTAAACCC 
ACTATGATGA TCAGCACCAC AGCACTGAAC ACTGCTCTGA TCCAGTGGCA TCCTCCAAGA 
GAGATTGTGG GAGAGTTGTT AGGATATCGT TTGCAGTACA AGCGAGTGGA TGAAGACAGG 
TTTATTACAA TTGATTTTAG CAAGAATGAA CATCACCACA CAGTGACTGG GCTACATAAA 
GGTGCAAGCT ATATATTCAA GCTTTCTGCA AAAAACCGAG CTGGAGCAGG GGAGGAATAT 
GAAAAGGAAA TTTCCACAGC TGAAGATTTT CCTAGTGGGT TTCCCCAAAA TCTGCAAGTA 
GTTGGACTTA CAACCTCTAC TACAGAAGTG TCCTGGGAAC CACCAGTGTT GTCTGAGCGC 
AATGGAAAGA TCACCAACTA CACAGTGGTG TATCGGGACA TAAATAGCCA GCAGGATCTC 
ACCAATGTCA CTCGCGACAC ACACATAACA TTGGTCAATC TCAAGCCAGA CACCACATAT 
GACATCAAGG TCAGGGCAAG AACAAACAAA GGCGCTGGCC CTCTAAGTCC TAGCATTCAA 
TCACGTACAA TGCCTGTTGA CCAAGTGTTT GCTAAGAATT TCAGAGTAAA CGCCGTGACA 
AAAACCTCAG TCTTGCTAAG CTGGGAGCTG CCTGATTCTT ACAAATCAGC TGTGCCTTTC 
AAGATCTTTT ACAATAATCA ACATGTAGAA GTTGATGGAC ACTCTATGCG CAAACTGATC 
AGTAATCTCA AGCCAGATAC AGAATACTCC TTTGTGCTGA TGAACAGGGG GAGCAGTGCA 
GGTGGACTGC AGCACCGCGT GTCCATTCGC ACAGCACCAG ATGTTTTAAA AAGCAAGCCT 
TTTGCTGCCA ACAAATACAT GGAGGAGGGC AGGTTCACCT TGCTGTTGCC TCAGGTTCAG 
ACTACTTCCC CAGTAAGGTG GTATTATATT GTGGTTGTCC CACTGGACAG TTCCAGCGGG 
GGTCTGAATT CTCGCTGGCA GAGCCCTGAG GAAATGGAGC TAGAGCAGAT ACTTGAAGCA 
ATAAACCAGG GATCCCGTCG TCATAGAAGA CAGGCAAGCA AGAATAAGCC ATACATTGCA 
GCCCGAGTAA ACACACTTCC AGAATCCTTT ATTTTGGGTG ACAATAAAGA ATATAATGGG 
TTCATCAACA AGCCACTGAA TCAGAATTTA CGCTACACCT GCTTTGTGCT AGCTGCACTA 
GAAGATGGGG ACACTAAAAA GTATGCAGCC AGCCCATACT CAGATGATAT TGTGGTGGAG 
GTGATCTCAC AGGCAAAACA ACAGGAAGAG CCAGAAATGC TTTGGGTCAT GGGTCCTGTT 
CTGGCTGTAA TTCTTATAAT AATTATAGTT ATTGCCATTC TACTTTTCAA AAGGAAAAGG 
ACCAACTCTC CTTCAGGCAA AGATGAACAG TCAGTAGGAC TTAAAGACTG TCTCCTCGCT 
CACTCCTCAG ATCCAGTTGA AATGAGGCGT CTAAACTACC AAACTCCAGG TATGAGAGAC 
CACCCTCCTA TTCCAATCTC TGACCTGGCA GACAACATTG AAAGACTGAA GGCTAATGAT 
GGACTCAAGT TCTCTCAGGA ATATGAGTCT ATTGATCCAG GTCAGCAATT CACGTGGGAA 
AATTCAAATC TAGAAGTAAA CAAACCAAAG AATCGCTATG CAAATGTCAT TGCATATGAC 
CATTCAAGGG TCATTCTTAC CTCAGTGGAT GGAGTTCCCG GAAGTGATTA CATTAATGCC 
AATTACATAG ATGGGTACAG GAAACAGAAT GCCTATATAG CTACGCAGGG CCCACTGCCA 
GAGACTATCA CTGATTTCTG GAGAATGGTG TGGGAGCAAA GAACCGCCAC TATTGTCATG 
ATGACCCGGC TAGAGGAAAA ATCAAGGGTG AAATGTGACC AGTACTGGCC CGGTCGTGGC 
TCAGAGACCT ATGGTATGAT CCAAGTCACA TTAATGGACA CAGTAGAGCT TGCCACCTAC 
ACAGTTAGGA CTTTCACACT TTATAAGAAT GGCTCCAGTG AGAAGCGGGA TGTCAGACAG 
TTCCAATTTA TGGCCTGGCC TGATCACGGG GTCCCTGAAT ATCCTACTCC TATTCTTGGA 
TTCCTTCGCA GAGTGAAAGG CTGTAATCCA CCTGATGCTG GTCCTATGGT TGTTCATTGC 
AGTGCTGGTG TGGGCAGAAC AGGATGCTTT ATTGTTATTG ATGCCATGCT AGAACGGGTG 
AAACTGGAGA AAACTGTGGA TATTTATGGT CATGTGACCT GCATGCGCTC CCAACGAAAT 
TATATGGTGC AAACAGAGGA CCAGTACATC TTTATCCATG AGGCTTTGCT TGAAGCAGTA 
ATGTGCGGGA TCACTGAAGT GCCAGCACGC AGTCTGTATG CACATATCCA GAAACTGTCT 
CAGGTCCCTC CGGGTGAGAG TGTTACCTCC ATGGAACTAG AGTTCAAGCT TTTGGCCAAC 
TCTAAAGCCC ACACCTCACG ATTTATCAGT GCCAACCTCC CATGCAACAA GTTCAAAAAC 
AGACTGGTAA ACATCATGCC ATATGAACTG AGTCGTGTCT CCCTGCAGCC CATTCGTGGT 
GTTGAGGGAT CAGATTATAT CAATGCAAGT TTCATTGATG GCTACAGGCA GCAGAAAGGC 
TACATTGCTA CTCAGGGTCC ATTGGCCGAG ACCACTGAGG ATTTCTGGAG AATGCTGTGG 
GAACATAACT CCACCATTGT GGTGATGCTG ACAAAGTTGC GAGAAATGGG GAGGGAGAAG 
TGCCATCAGT ACTGGCCTGC AGAGCGCTCA GCACGGTACC AGTATTTTGT GGTGGACCCT 
ATGGCTGAGT ATAACATGCC TCAGTATATC CTGCGTGAGT TCAAAGTGAC AGATGCACGG 
GATGGCCAAT CTCGTACAAT TAGACAATTC CAGTTCACTG ATTGGCCTGA ACAAGGAGTG 
CCAAAAACAG GTGAAGGCTT TATTGACTTC ATTGGGCAGG TGCACAAAAC CAAGGAGCAG 
TTTGGGCAGG ATGGGCCAAT CACCGTTCAC TGCAGCGCTG GCGTGGGGCG CACTGGAGTA 
TTCATCACTC TCAGTATTGT ACTGGAGCGT ATGAGGTACG AGGGTGTGGT TGACATGTTT 
CAAACAGTGA AGACTTTAAG AACACAGCGG CCAGCAATGG TACAAACAGA GGACCAGTAT 
CAACTGTGTT ATAGAGCCGC ACTGGAGTAT CTGGGCAGCT TTGACCACTA TGCAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_004913963.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914020.1 Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X4, mRNA.

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CloneID	OXa09450
Clone ID Related Accession (Same CDS sequence)	XM_004913963.1
Accession Version	XM_004913963.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTCAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACATT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGAGATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCAAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTAACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTGCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA AGATGCTGTT ATCACTGGCC TCTCTCCTGA GACCACTTAT 
TCCATCACTG TGGCCGCCTA CACTACTAAG GGAGATGGTG CTAGAAGCAA ACCCAAGGTC 
GTTAGCACCA CAGGAGCTGT GCCTGGTAAA CCCACTATGA TGATCAGCAC CACAGCACTG 
AACACTGCTC TGATCCAGTG GCATCCTCCA AGAGAGATTG TGGGAGAGTT GTTAGGATAT 
CGTTTGCAGT ACAAGCGAGT GGATGAAGAC AGGTTTATTA CAATTGATTT TAGCAAGAAT 
GAACATCACC ACACAGTGAC TGGGCTACAT AAAGGTGCAA GCTATATATT CAAGCTTTCT 
GCAAAAAACC GAGCTGGAGC AGGGGAGGAA TATGAAAAGG AAATTTCCAC AGCTGAAGAT 
TTTCCTAGTG GGTTTCCCCA AAATCTGCAA GTAGTTGGAC TTACAACCTC TACTACAGAA 
GTGTCCTGGG AACCACCAGT GTTGTCTGAG CGCAATGGAA AGATCACCAA CTACACAGTG 
GTGTATCGGG ACATAAATAG CCAGCAGGAT CTCACCAATG TCACTCGCGA CACACACATA 
ACATTGGTCA ATCTCAAGCC AGACACCACA TATGACATCA AGGTCAGGGC AAGAACAAAC 
AAAGGCGCTG GCCCTCTAAG TCCTAGCATT CAATCACGTA CAATGCCTGT TGACCAAGTG 
TTTGCTAAGA ATTTCAGAGT AAACGCCGTG ACAAAAACCT CAGTCTTGCT AAGCTGGGAG 
CTGCCTGATT CTTACAAATC AGCTGTGCCT TTCAAGATCT TTTACAATAA TCAACATGTA 
GAAGTTGATG GACACTCTAT GCGCAAACTG ATCAGTAATC TCAAGCCAGA TACAGAATAC 
TCCTTTGTGC TGATGAACAG GGGGAGCAGT GCAGGTGGAC TGCAGCACCG CGTGTCCATT 
CGCACAGCAC CAGATGTTTT AAAAAGCAAG CCTTTTGCTG CCAACAAATA CATGGAGGAG 
GGCAGGTTCA CCTTGCTGTT GCCTCAGGTT CAGACTACTT CCCCAGTAAG GTGGTATTAT 
ATTGTGGTTG TCCCACTGGA CAGTTCCAGC GGGGGTCTGA ATTCTCGCTG GCAGAGCCCT 
GAGGAAATGG AGCTAGAGCA GATACTTGAA GCAATAAACC AGGGATCCCG TCGTCATAGA 
AGACAGGCAA GCAAGAATAA GCCATACATT GCAGCCCGAG TAAACACACT TCCAGAATCC 
TTTATTTTGG GTGACAATAA AGAATATAAT GGGTTCATCA ACAAGCCACT GAATCAGAAT 
TTACGCTACA CCTGCTTTGT GCTAGCTGCA CTAGAAGATG GGGACACTAA AAAGTATGCA 
GCCAGCCCAT ACTCAGATGA TATTGTGGTG GAGGTGATCT CACAGGCAAA ACAACAGGAA 
GAGCCAGAAA TGCTTTGGGT CATGGGTCCT GTTCTGGCTG TAATTCTTAT AATAATTATA 
GTTATTGCCA TTCTACTTTT CAAAAGGAAA AGGACCAACT CTCCTTCAGG CAAAGATGAA 
CAGTCAGTAG GACTTAAAGA CTGTCTCCTC GCTCACTCCT CAGATCCAGT TGAAATGAGG 
CGTCTAAACT ACCAAACTCC AGGTATGAGA GACCACCCTC CTATTCCAAT CTCTGACCTG 
GCAGACAACA TTGAAAGACT GAAGGCTAAT GATGGACTCA AGTTCTCTCA GGAATATGAG 
TCTATTGATC CAGGTCAGCA ATTCACGTGG GAAAATTCAA ATCTAGAAGT AAACAAACCA 
AAGAATCGCT ATGCAAATGT CATTGCATAT GACCATTCAA GGGTCATTCT TACCTCAGTG 
GATGGAGTTC CCGGAAGTGA TTACATTAAT GCCAATTACA TAGATGGGTA CAGGAAACAG 
AATGCCTATA TAGCTACGCA GGGCCCACTG CCAGAGACTA TCACTGATTT CTGGAGAATG 
GTGTGGGAGC AAAGAACCGC CACTATTGTC ATGATGACCC GGCTAGAGGA AAAATCAAGG 
GTGAAATGTG ACCAGTACTG GCCCGGTCGT GGCTCAGAGA CCTATGGTAT GATCCAAGTC 
ACATTAATGG ACACAGTAGA GCTTGCCACC TACACAGTTA GGACTTTCAC ACTTTATAAG 
AATGGCTCCA GTGAGAAGCG GGATGTCAGA CAGTTCCAAT TTATGGCCTG GCCTGATCAC 
GGGGTCCCTG AATATCCTAC TCCTATTCTT GGATTCCTTC GCAGAGTGAA AGGCTGTAAT 
CCACCTGATG CTGGTCCTAT GGTTGTTCAT TGCAGTGCTG GTGTGGGCAG AACAGGATGC 
TTTATTGTTA TTGATGCCAT GCTAGAACGG GTGAAACTGG AGAAAACTGT GGATATTTAT 
GGTCATGTGA CCTGCATGCG CTCCCAACGA AATTATATGG TGCAAACAGA GGACCAGTAC 
ATCTTTATCC ATGAGGCTTT GCTTGAAGCA GTAATGTGCG GGATCACTGA AGTGCCAGCA 
CGCAGTCTGT ATGCACATAT CCAGAAACTG TCTCAGGTCC CTCCGGGTGA GAGTGTTACC 
TCCATGGAAC TAGAGTTCAA GCTTTTGGCC AACTCTAAAG CCCACACCTC ACGATTTATC 
AGTGCCAACC TCCCATGCAA CAAGTTCAAA AACAGACTGG TAAACATCAT GCCATATGAA 
CTGAGTCGTG TCTCCCTGCA GCCCATTCGT GGTGTTGAGG GATCAGATTA TATCAATGCA 
AGTTTCATTG ATGGCTACAG GCAGCAGAAA GGCTACATTG CTACTCAGGG TCCATTGGCC 
GAGACCACTG AGGATTTCTG GAGAATGCTG TGGGAACATA ACTCCACCAT TGTGGTGATG 
CTGACAAAGT TGCGAGAAAT GGGGAGGGAG AAGTGCCATC AGTACTGGCC TGCAGAGCGC 
TCAGCACGGT ACCAGTATTT TGTGGTGGAC CCTATGGCTG AGTATAACAT GCCTCAGTAT 
ATCCTGCGTG AGTTCAAAGT GACAGATGCA CGGGATGGCC AATCTCGTAC AATTAGACAA 
TTCCAGTTCA CTGATTGGCC TGAACAAGGA GTGCCAAAAA CAGGTGAAGG CTTTATTGAC 
TTCATTGGGC AGGTGCACAA AACCAAGGAG CAGTTTGGGC AGGATGGGCC AATCACCGTT 
CACTGCAGCG CTGGCGTGGG GCGCACTGGA GTATTCATCA CTCTCAGTAT TGTACTGGAG 
CGTATGAGGT ACGAGGGTGT GGTTGACATG TTTCAAACAG TGAAGACTTT AAGAACACAG 
CGGCCAGCAA TGGTACAAAC AGAGGACCAG TATCAACTGT GTTATAGAGC CGCACTGGAG 
TATCTGGGCA GCTTTGACCA CTATGCAACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914020.1
CDS	635..6307
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSEIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKRKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_004913963.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913963.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTCAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACATT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGAGATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCAAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTAACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTGCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA AGATGCTGTT ATCACTGGCC TCTCTCCTGA GACCACTTAT 
TCCATCACTG TGGCCGCCTA CACTACTAAG GGAGATGGTG CTAGAAGCAA ACCCAAGGTC 
GTTAGCACCA CAGGAGCTGT GCCTGGTAAA CCCACTATGA TGATCAGCAC CACAGCACTG 
AACACTGCTC TGATCCAGTG GCATCCTCCA AGAGAGATTG TGGGAGAGTT GTTAGGATAT 
CGTTTGCAGT ACAAGCGAGT GGATGAAGAC AGGTTTATTA CAATTGATTT TAGCAAGAAT 
GAACATCACC ACACAGTGAC TGGGCTACAT AAAGGTGCAA GCTATATATT CAAGCTTTCT 
GCAAAAAACC GAGCTGGAGC AGGGGAGGAA TATGAAAAGG AAATTTCCAC AGCTGAAGAT 
TTTCCTAGTG GGTTTCCCCA AAATCTGCAA GTAGTTGGAC TTACAACCTC TACTACAGAA 
GTGTCCTGGG AACCACCAGT GTTGTCTGAG CGCAATGGAA AGATCACCAA CTACACAGTG 
GTGTATCGGG ACATAAATAG CCAGCAGGAT CTCACCAATG TCACTCGCGA CACACACATA 
ACATTGGTCA ATCTCAAGCC AGACACCACA TATGACATCA AGGTCAGGGC AAGAACAAAC 
AAAGGCGCTG GCCCTCTAAG TCCTAGCATT CAATCACGTA CAATGCCTGT TGACCAAGTG 
TTTGCTAAGA ATTTCAGAGT AAACGCCGTG ACAAAAACCT CAGTCTTGCT AAGCTGGGAG 
CTGCCTGATT CTTACAAATC AGCTGTGCCT TTCAAGATCT TTTACAATAA TCAACATGTA 
GAAGTTGATG GACACTCTAT GCGCAAACTG ATCAGTAATC TCAAGCCAGA TACAGAATAC 
TCCTTTGTGC TGATGAACAG GGGGAGCAGT GCAGGTGGAC TGCAGCACCG CGTGTCCATT 
CGCACAGCAC CAGATGTTTT AAAAAGCAAG CCTTTTGCTG CCAACAAATA CATGGAGGAG 
GGCAGGTTCA CCTTGCTGTT GCCTCAGGTT CAGACTACTT CCCCAGTAAG GTGGTATTAT 
ATTGTGGTTG TCCCACTGGA CAGTTCCAGC GGGGGTCTGA ATTCTCGCTG GCAGAGCCCT 
GAGGAAATGG AGCTAGAGCA GATACTTGAA GCAATAAACC AGGGATCCCG TCGTCATAGA 
AGACAGGCAA GCAAGAATAA GCCATACATT GCAGCCCGAG TAAACACACT TCCAGAATCC 
TTTATTTTGG GTGACAATAA AGAATATAAT GGGTTCATCA ACAAGCCACT GAATCAGAAT 
TTACGCTACA CCTGCTTTGT GCTAGCTGCA CTAGAAGATG GGGACACTAA AAAGTATGCA 
GCCAGCCCAT ACTCAGATGA TATTGTGGTG GAGGTGATCT CACAGGCAAA ACAACAGGAA 
GAGCCAGAAA TGCTTTGGGT CATGGGTCCT GTTCTGGCTG TAATTCTTAT AATAATTATA 
GTTATTGCCA TTCTACTTTT CAAAAGGAAA AGGACCAACT CTCCTTCAGG CAAAGATGAA 
CAGTCAGTAG GACTTAAAGA CTGTCTCCTC GCTCACTCCT CAGATCCAGT TGAAATGAGG 
CGTCTAAACT ACCAAACTCC AGGTATGAGA GACCACCCTC CTATTCCAAT CTCTGACCTG 
GCAGACAACA TTGAAAGACT GAAGGCTAAT GATGGACTCA AGTTCTCTCA GGAATATGAG 
TCTATTGATC CAGGTCAGCA ATTCACGTGG GAAAATTCAA ATCTAGAAGT AAACAAACCA 
AAGAATCGCT ATGCAAATGT CATTGCATAT GACCATTCAA GGGTCATTCT TACCTCAGTG 
GATGGAGTTC CCGGAAGTGA TTACATTAAT GCCAATTACA TAGATGGGTA CAGGAAACAG 
AATGCCTATA TAGCTACGCA GGGCCCACTG CCAGAGACTA TCACTGATTT CTGGAGAATG 
GTGTGGGAGC AAAGAACCGC CACTATTGTC ATGATGACCC GGCTAGAGGA AAAATCAAGG 
GTGAAATGTG ACCAGTACTG GCCCGGTCGT GGCTCAGAGA CCTATGGTAT GATCCAAGTC 
ACATTAATGG ACACAGTAGA GCTTGCCACC TACACAGTTA GGACTTTCAC ACTTTATAAG 
AATGGCTCCA GTGAGAAGCG GGATGTCAGA CAGTTCCAAT TTATGGCCTG GCCTGATCAC 
GGGGTCCCTG AATATCCTAC TCCTATTCTT GGATTCCTTC GCAGAGTGAA AGGCTGTAAT 
CCACCTGATG CTGGTCCTAT GGTTGTTCAT TGCAGTGCTG GTGTGGGCAG AACAGGATGC 
TTTATTGTTA TTGATGCCAT GCTAGAACGG GTGAAACTGG AGAAAACTGT GGATATTTAT 
GGTCATGTGA CCTGCATGCG CTCCCAACGA AATTATATGG TGCAAACAGA GGACCAGTAC 
ATCTTTATCC ATGAGGCTTT GCTTGAAGCA GTAATGTGCG GGATCACTGA AGTGCCAGCA 
CGCAGTCTGT ATGCACATAT CCAGAAACTG TCTCAGGTCC CTCCGGGTGA GAGTGTTACC 
TCCATGGAAC TAGAGTTCAA GCTTTTGGCC AACTCTAAAG CCCACACCTC ACGATTTATC 
AGTGCCAACC TCCCATGCAA CAAGTTCAAA AACAGACTGG TAAACATCAT GCCATATGAA 
CTGAGTCGTG TCTCCCTGCA GCCCATTCGT GGTGTTGAGG GATCAGATTA TATCAATGCA 
AGTTTCATTG ATGGCTACAG GCAGCAGAAA GGCTACATTG CTACTCAGGG TCCATTGGCC 
GAGACCACTG AGGATTTCTG GAGAATGCTG TGGGAACATA ACTCCACCAT TGTGGTGATG 
CTGACAAAGT TGCGAGAAAT GGGGAGGGAG AAGTGCCATC AGTACTGGCC TGCAGAGCGC 
TCAGCACGGT ACCAGTATTT TGTGGTGGAC CCTATGGCTG AGTATAACAT GCCTCAGTAT 
ATCCTGCGTG AGTTCAAAGT GACAGATGCA CGGGATGGCC AATCTCGTAC AATTAGACAA 
TTCCAGTTCA CTGATTGGCC TGAACAAGGA GTGCCAAAAA CAGGTGAAGG CTTTATTGAC 
TTCATTGGGC AGGTGCACAA AACCAAGGAG CAGTTTGGGC AGGATGGGCC AATCACCGTT 
CACTGCAGCG CTGGCGTGGG GCGCACTGGA GTATTCATCA CTCTCAGTAT TGTACTGGAG 
CGTATGAGGT ACGAGGGTGT GGTTGACATG TTTCAAACAG TGAAGACTTT AAGAACACAG 
CGGCCAGCAA TGGTACAAAC AGAGGACCAG TATCAACTGT GTTATAGAGC CGCACTGGAG 
TATCTGGGCA GCTTTGACCA CTATGCAACA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptprf ( XM_012961577.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817031.1 Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X1, mRNA.

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CloneID	OXa35597
Clone ID Related Accession (Same CDS sequence)	XM_012961577.1
Accession Version	XM_012961577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5712bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTCAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACATT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGAGATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCAAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTAACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTGCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA ATGGAGACCT GAGGATGTGG GAGATTACGA TGCTGTTATC 
ACTGGCCTCT CTCCTGAGAC CACTTATTCC ATCACTGTGG CCGCCTACAC TACTAAGGGA 
GATGGTGCTA GAAGCAAACC CAAGGTCGTT AGCACCACAG GAGCTGTGCC TGGTAAACCC 
ACTATGATGA TCAGCACCAC AGCACTGAAC ACTGCTCTGA TCCAGTGGCA TCCTCCAAGA 
GAGATTGTGG GAGAGTTGTT AGGATATCGT TTGCAGTACA AGCGAGTGGA TGAAGACAGG 
TTTATTACAA TTGATTTTAG CAAGAATGAA CATCACCACA CAGTGACTGG GCTACATAAA 
GGTGCAAGCT ATATATTCAA GCTTTCTGCA AAAAACCGAG CTGGAGCAGG GGAGGAATAT 
GAAAAGGAAA TTTCCACAGC TGAAGATTTT CCTAGTGGGT TTCCCCAAAA TCTGCAAGTA 
GTTGGACTTA CAACCTCTAC TACAGAAGTG TCCTGGGAAC CACCAGTGTT GTCTGAGCGC 
AATGGAAAGA TCACCAACTA CACAGTGGTG TATCGGGACA TAAATAGCCA GCAGGATCTC 
ACCAATGTCA CTCGCGACAC ACACATAACA TTGGTCAATC TCAAGCCAGA CACCACATAT 
GACATCAAGG TCAGGGCAAG AACAAACAAA GGCGCTGGCC CTCTAAGTCC TAGCATTCAA 
TCACGTACAA TGCCTGTTGA CCAAGTGTTT GCTAAGAATT TCAGAGTAAA CGCCGTGACA 
AAAACCTCAG TCTTGCTAAG CTGGGAGCTG CCTGATTCTT ACAAATCAGC TGTGCCTTTC 
AAGATCTTTT ACAATAATCA ACATGTAGAA GTTGATGGAC ACTCTATGCG CAAACTGATC 
AGTAATCTCA AGCCAGATAC AGAATACTCC TTTGTGCTGA TGAACAGGGG GAGCAGTGCA 
GGTGGACTGC AGCACCGCGT GTCCATTCGC ACAGCACCAG ATGTTTTAAA AAGCAAGCCT 
TTTGCTGCCA ACAAATACAT GGAGGAGGGC AGGTTCACCT TGCTGTTGCC TCAGGTTCAG 
ACTACTTCCC CAGTAAGGTG GTATTATATT GTGGTTGTCC CACTGGACAG TTCCAGCGGG 
GGTCTGAATT CTCGCTGGCA GAGCCCTGAG GAAATGGAGC TAGAGCAGAT ACTTGAAGCA 
ATAAACCAGG GATCCCGTCG TCATAGAAGA CAGGCAAGCA AGAATAAGCC ATACATTGCA 
GCCCGAGTAA ACACACTTCC AGAATCCTTT ATTTTGGGTG ACAATAAAGA ATATAATGGG 
TTCATCAACA AGCCACTGAA TCAGAATTTA CGCTACACCT GCTTTGTGCT AGCTGCACTA 
GAAGATGGGG ACACTAAAAA GTATGCAGCC AGCCCATACT CAGATGATAT TGTGGTGGAG 
GTGATCTCAC AGGCAAAACA ACAGGAAGAG CCAGAAATGC TTTGGGTCAT GGGTCCTGTT 
CTGGCTGTAA TTCTTATAAT AATTATAGTT ATTGCCATTC TACTTTTCAA AAGTAAACAG 
GAAAGGAAAA GGACCAACTC TCCTTCAGGC AAAGATGAAC AGTCAGTAGG ACTTAAAGAC 
TGTCTCCTCG CTCACTCCTC AGATCCAGTT GAAATGAGGC GTCTAAACTA CCAAACTCCA 
GGTATGAGAG ACCACCCTCC TATTCCAATC TCTGACCTGG CAGACAACAT TGAAAGACTG 
AAGGCTAATG ATGGACTCAA GTTCTCTCAG GAATATGAGT CTATTGATCC AGGTCAGCAA 
TTCACGTGGG AAAATTCAAA TCTAGAAGTA AACAAACCAA AGAATCGCTA TGCAAATGTC 
ATTGCATATG ACCATTCAAG GGTCATTCTT ACCTCAGTGG ATGGAGTTCC CGGAAGTGAT 
TACATTAATG CCAATTACAT AGATGGGTAC AGGAAACAGA ATGCCTATAT AGCTACGCAG 
GGCCCACTGC CAGAGACTAT CACTGATTTC TGGAGAATGG TGTGGGAGCA AAGAACCGCC 
ACTATTGTCA TGATGACCCG GCTAGAGGAA AAATCAAGGG TGAAATGTGA CCAGTACTGG 
CCCGGTCGTG GCTCAGAGAC CTATGGTATG ATCCAAGTCA CATTAATGGA CACAGTAGAG 
CTTGCCACCT ACACAGTTAG GACTTTCACA CTTTATAAGA ATGGCTCCAG TGAGAAGCGG 
GATGTCAGAC AGTTCCAATT TATGGCCTGG CCTGATCACG GGGTCCCTGA ATATCCTACT 
CCTATTCTTG GATTCCTTCG CAGAGTGAAA GGCTGTAATC CACCTGATGC TGGTCCTATG 
GTTGTTCATT GCAGTGCTGG TGTGGGCAGA ACAGGATGCT TTATTGTTAT TGATGCCATG 
CTAGAACGGG TGAAACTGGA GAAAACTGTG GATATTTATG GTCATGTGAC CTGCATGCGC 
TCCCAACGAA ATTATATGGT GCAAACAGAG GACCAGTACA TCTTTATCCA TGAGGCTTTG 
CTTGAAGCAG TAATGTGCGG GATCACTGAA GTGCCAGCAC GCAGTCTGTA TGCACATATC 
CAGAAACTGT CTCAGGTCCC TCCGGGTGAG AGTGTTACCT CCATGGAACT AGAGTTCAAG 
CTTTTGGCCA ACTCTAAAGC CCACACCTCA CGATTTATCA GTGCCAACCT CCCATGCAAC 
AAGTTCAAAA ACAGACTGGT AAACATCATG CCATATGAAC TGAGTCGTGT CTCCCTGCAG 
CCCATTCGTG GTGTTGAGGG ATCAGATTAT ATCAATGCAA GTTTCATTGA TGGCTACAGG 
CAGCAGAAAG GCTACATTGC TACTCAGGGT CCATTGGCCG AGACCACTGA GGATTTCTGG 
AGAATGCTGT GGGAACATAA CTCCACCATT GTGGTGATGC TGACAAAGTT GCGAGAAATG 
GGGAGGGAGA AGTGCCATCA GTACTGGCCT GCAGAGCGCT CAGCACGGTA CCAGTATTTT 
GTGGTGGACC CTATGGCTGA GTATAACATG CCTCAGTATA TCCTGCGTGA GTTCAAAGTG 
ACAGATGCAC GGGATGGCCA ATCTCGTACA ATTAGACAAT TCCAGTTCAC TGATTGGCCT 
GAACAAGGAG TGCCAAAAAC AGGTGAAGGC TTTATTGACT TCATTGGGCA GGTGCACAAA 
ACCAAGGAGC AGTTTGGGCA GGATGGGCCA ATCACCGTTC ACTGCAGCGC TGGCGTGGGG 
CGCACTGGAG TATTCATCAC TCTCAGTATT GTACTGGAGC GTATGAGGTA CGAGGGTGTG 
GTTGACATGT TTCAAACAGT GAAGACTTTA AGAACACAGC GGCCAGCAAT GGTACAAACA 
GAGGACCAGT ATCAACTGTG TTATAGAGCC GCACTGGAGT ATCTGGGCAG CTTTGACCAC 
TATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817031.1
CDS	635..6346
Translation	MVPCSCLSLLILSAIVLCCSITLTQADNEPVFVRKPDDQIGISGGVASFVCQATADPKPRITWMKKGKKVSSQRFEVIEFDDGSGSVLRIQPLRVHRDEAIYECTATNSVGEINSSAKLTVLEEEQLPHGFPVIDMGPQLKVVEKTRTATMLCAASGNPDPEITWFKDFLPVDTASSNGRIKQLRSGALQIENSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSNHEVMPGGSVNLTCVAVGAPMPYVKWMAGLEELTKEDEMPVGRNVLELTNIKESANYTCVAISSLGMIEAVAQITVKALPKPPLDGTVTETTATSVTLTWDSGNPDPVSYYVIQYKPKASESSFQEVDGVATTRYSIGGLSPFSEYEFRIIAVNNIGRGPPSEVIEAQTGEQAPSSPPLKVQARMLSASTMLVQWDLPEEPNGQIRGFRVYYTTDPHLPFSMWQKHNVVDNHLTTIGGLTTGTTYSIRVLAFTSVGDGPPSEIIQVKTQQGVPAQPVDFQAEAETDTRILLTWLLPPQERITKYELLYWEGTDGEQQKLMFHPTSSYAVEGLKPDTLYHFRLAAWSDTGVGVYTPAIQARTAQSTPSAPPQDVECSSLSSTAIRVSWVAPPASSRNGALTRYSLAYQALAGEDTSRHVVDSISPDISSWEIKDLEKWTEYKVWVRAHTDVGAGPESSPIIVRTDEDVPSAPPRKVEVDSVNSTSVRVTWKSPLPTKQNGQIRGYQVTYVRLEGGEPRGNPIIKDVMLSEAQWRPEDVGDYDAVITGLSPETTYSITVAAYTTKGDGARSKPKVVSTTGAVPGKPTMMISTTALNTALIQWHPPREIVGELLGYRLQYKRVDEDRFITIDFSKNEHHHTVTGLHKGASYIFKLSAKNRAGAGEEYEKEISTAEDFPSGFPQNLQVVGLTTSTTEVSWEPPVLSERNGKITNYTVVYRDINSQQDLTNVTRDTHITLVNLKPDTTYDIKVRARTNKGAGPLSPSIQSRTMPVDQVFAKNFRVNAVTKTSVLLSWELPDSYKSAVPFKIFYNNQHVEVDGHSMRKLISNLKPDTEYSFVLMNRGSSAGGLQHRVSIRTAPDVLKSKPFAANKYMEEGRFTLLLPQVQTTSPVRWYYIVVVPLDSSSGGLNSRWQSPEEMELEQILEAINQGSRRHRRQASKNKPYIAARVNTLPESFILGDNKEYNGFINKPLNQNLRYTCFVLAALEDGDTKKYAASPYSDDIVVEVISQAKQQEEPEMLWVMGPVLAVILIIIIVIAILLFKSKQERKRTNSPSGKDEQSVGLKDCLLAHSSDPVEMRRLNYQTPGMRDHPPIPISDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETITDFWRMVWEQRTATIVMMTRLEEKSRVKCDQYWPGRGSETYGMIQVTLMDTVELATYTVRTFTLYKNGSSEKRDVRQFQFMAWPDHGVPEYPTPILGFLRRVKGCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERVKLEKTVDIYGHVTCMRSQRNYMVQTEDQYIFIHEALLEAVMCGITEVPARSLYAHIQKLSQVPPGESVTSMELEFKLLANSKAHTSRFISANLPCNKFKNRLVNIMPYELSRVSLQPIRGVEGSDYINASFIDGYRQQKGYIATQGPLAETTEDFWRMLWEHNSTIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_012961577.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine phosphatase, receptor type F (ptprf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961577.1

ORF Insert Sequence:

ATGGTGCCTT GCTCATGCTT GTCCCTGTTG ATATTGTCGG CAATAGTGCT TTGCTGCTCA 
ATCACGTTGA CACAAGCAGA CAATGAGCCA GTCTTTGTCA GGAAGCCCGA TGACCAGATT 
GGAATATCAG GTGGTGTTGC GTCTTTTGTG TGCCAAGCGA CAGCAGACCC CAAGCCACGG 
ATTACCTGGA TGAAGAAGGG GAAGAAAGTC AGTTCCCAGC GCTTTGAGGT AATAGAGTTT 
GACGATGGAT CGGGATCTGT CCTTCGAATT CAACCTCTCC GTGTACACAG AGACGAAGCA 
ATATACGAGT GCACTGCAAC CAACAGCGTT GGTGAGATCA ACAGCAGTGC TAAGCTAACA 
GTACTAGAAG AGGAGCAGCT TCCCCATGGA TTTCCTGTCA TTGACATGGG GCCTCAACTA 
AAAGTCGTGG AAAAAACTCG CACAGCAACC ATGCTTTGTG CAGCCAGTGG CAATCCTGAT 
CCAGAAATAA CCTGGTTCAA GGATTTTCTT CCTGTAGACA CGGCATCAAG TAATGGTCGA 
ATTAAGCAGC TCCGTTCAGG TGCCTTGCAG ATAGAAAACA GCGAGGAATC TGATCAGGGC 
AAATATGAAT GTGTTGCAAC CAACAGTGCA GGGACACGTT ACTCAGCTCC TGCTAACCTC 
TATGTTCGTG TCCGTCGAGT GGCACCAAGA TTTTCTATCC CTCCCAGTAA CCATGAAGTG 
ATGCCTGGGG GCAGTGTTAA TTTAACATGT GTAGCAGTGG GGGCCCCTAT GCCCTATGTC 
AAATGGATGG CTGGCCTTGA GGAACTCACC AAAGAAGATG AAATGCCTGT GGGCCGCAAT 
GTTTTAGAAC TGACCAATAT CAAAGAGTCT GCCAATTACA CCTGTGTTGC GATCTCCTCA 
TTAGGCATGA TTGAAGCAGT TGCACAGATC ACTGTGAAAG CATTGCCAAA ACCGCCTTTG 
GATGGTACAG TAACAGAGAC TACAGCCACT AGTGTCACCC TAACTTGGGA TTCTGGCAAC 
CCAGACCCAG TCTCTTATTA TGTGATTCAG TATAAACCCA AAGCTTCAGA AAGCTCATTT 
CAAGAAGTTG ATGGAGTGGC AACAACTCGG TACAGTATTG GGGGACTCAG CCCCTTCTCA 
GAGTATGAAT TTCGCATCAT TGCCGTAAAC AATATTGGCC GAGGACCTCC AAGTGAAGTG 
ATAGAGGCTC AGACAGGGGA ACAGGCACCC TCAAGCCCAC CTTTGAAGGT CCAAGCTCGT 
ATGTTAAGTG CCAGTACTAT GCTAGTTCAG TGGGACCTAC CAGAAGAACC AAATGGCCAG 
ATAAGAGGTT TCCGGGTTTA TTACACTACT GATCCACATT TGCCTTTTAG TATGTGGCAA 
AAACACAATG TTGTTGACAA CCACTTGACA ACAATAGGAG GTCTGACCAC AGGAACCACT 
TACAGCATTC GTGTTCTTGC CTTCACATCT GTCGGGGATG GACCGCCTTC TGAGATAATT 
CAGGTCAAAA CACAGCAGGG AGTTCCTGCG CAGCCTGTTG ATTTCCAGGC AGAAGCTGAG 
ACTGACACAC GCATATTGCT CACGTGGCTT CTCCCACCAC AAGAACGCAT TACCAAATAT 
GAGCTGCTTT ACTGGGAAGG AACAGATGGA GAACAGCAAA AGCTTATGTT TCACCCTACC 
TCCTCTTATG CTGTGGAGGG TCTGAAACCG GACACACTTT ATCATTTCCG ATTAGCTGCG 
TGGTCTGACA CTGGAGTTGG TGTTTACACC CCAGCAATTC AAGCTAGAAC TGCACAATCT 
ACACCCTCAG CCCCGCCACA GGATGTGGAG TGCTCAAGTC TTAGCTCCAC TGCCATCCGG 
GTAAGTTGGG TGGCCCCTCC AGCTTCCAGC CGCAACGGGG CCCTAACCCG CTACTCACTT 
GCCTACCAAG CCCTGGCTGG GGAGGACACA TCCCGGCATG TTGTGGACAG CATCTCACCA 
GATATCTCCA GCTGGGAGAT CAAGGATCTG GAGAAATGGA CTGAGTACAA AGTATGGGTG 
CGAGCTCATA CAGATGTAGG GGCTGGGCCT GAAAGCTCCC CCATCATTGT GCGGACAGAT 
GAGGATGTGC CGAGTGCACC ACCAAGAAAG GTTGAAGTAG ACTCTGTAAA CTCCACATCT 
GTGCGTGTGA CATGGAAGTC TCCTCTACCA ACCAAGCAAA ATGGGCAAAT TAGGGGGTAT 
CAAGTCACTT ATGTTCGTCT AGAAGGTGGA GAGCCTCGGG GGAATCCCAT CATAAAAGAT 
GTTATGCTGT CAGAGGCTCA ATGGAGACCT GAGGATGTGG GAGATTACGA TGCTGTTATC 
ACTGGCCTCT CTCCTGAGAC CACTTATTCC ATCACTGTGG CCGCCTACAC TACTAAGGGA 
GATGGTGCTA GAAGCAAACC CAAGGTCGTT AGCACCACAG GAGCTGTGCC TGGTAAACCC 
ACTATGATGA TCAGCACCAC AGCACTGAAC ACTGCTCTGA TCCAGTGGCA TCCTCCAAGA 
GAGATTGTGG GAGAGTTGTT AGGATATCGT TTGCAGTACA AGCGAGTGGA TGAAGACAGG 
TTTATTACAA TTGATTTTAG CAAGAATGAA CATCACCACA CAGTGACTGG GCTACATAAA 
GGTGCAAGCT ATATATTCAA GCTTTCTGCA AAAAACCGAG CTGGAGCAGG GGAGGAATAT 
GAAAAGGAAA TTTCCACAGC TGAAGATTTT CCTAGTGGGT TTCCCCAAAA TCTGCAAGTA 
GTTGGACTTA CAACCTCTAC TACAGAAGTG TCCTGGGAAC CACCAGTGTT GTCTGAGCGC 
AATGGAAAGA TCACCAACTA CACAGTGGTG TATCGGGACA TAAATAGCCA GCAGGATCTC 
ACCAATGTCA CTCGCGACAC ACACATAACA TTGGTCAATC TCAAGCCAGA CACCACATAT 
GACATCAAGG TCAGGGCAAG AACAAACAAA GGCGCTGGCC CTCTAAGTCC TAGCATTCAA 
TCACGTACAA TGCCTGTTGA CCAAGTGTTT GCTAAGAATT TCAGAGTAAA CGCCGTGACA 
AAAACCTCAG TCTTGCTAAG CTGGGAGCTG CCTGATTCTT ACAAATCAGC TGTGCCTTTC 
AAGATCTTTT ACAATAATCA ACATGTAGAA GTTGATGGAC ACTCTATGCG CAAACTGATC 
AGTAATCTCA AGCCAGATAC AGAATACTCC TTTGTGCTGA TGAACAGGGG GAGCAGTGCA 
GGTGGACTGC AGCACCGCGT GTCCATTCGC ACAGCACCAG ATGTTTTAAA AAGCAAGCCT 
TTTGCTGCCA ACAAATACAT GGAGGAGGGC AGGTTCACCT TGCTGTTGCC TCAGGTTCAG 
ACTACTTCCC CAGTAAGGTG GTATTATATT GTGGTTGTCC CACTGGACAG TTCCAGCGGG 
GGTCTGAATT CTCGCTGGCA GAGCCCTGAG GAAATGGAGC TAGAGCAGAT ACTTGAAGCA 
ATAAACCAGG GATCCCGTCG TCATAGAAGA CAGGCAAGCA AGAATAAGCC ATACATTGCA 
GCCCGAGTAA ACACACTTCC AGAATCCTTT ATTTTGGGTG ACAATAAAGA ATATAATGGG 
TTCATCAACA AGCCACTGAA TCAGAATTTA CGCTACACCT GCTTTGTGCT AGCTGCACTA 
GAAGATGGGG ACACTAAAAA GTATGCAGCC AGCCCATACT CAGATGATAT TGTGGTGGAG 
GTGATCTCAC AGGCAAAACA ACAGGAAGAG CCAGAAATGC TTTGGGTCAT GGGTCCTGTT 
CTGGCTGTAA TTCTTATAAT AATTATAGTT ATTGCCATTC TACTTTTCAA AAGTAAACAG 
GAAAGGAAAA GGACCAACTC TCCTTCAGGC AAAGATGAAC AGTCAGTAGG ACTTAAAGAC 
TGTCTCCTCG CTCACTCCTC AGATCCAGTT GAAATGAGGC GTCTAAACTA CCAAACTCCA 
GGTATGAGAG ACCACCCTCC TATTCCAATC TCTGACCTGG CAGACAACAT TGAAAGACTG 
AAGGCTAATG ATGGACTCAA GTTCTCTCAG GAATATGAGT CTATTGATCC AGGTCAGCAA 
TTCACGTGGG AAAATTCAAA TCTAGAAGTA AACAAACCAA AGAATCGCTA TGCAAATGTC 
ATTGCATATG ACCATTCAAG GGTCATTCTT ACCTCAGTGG ATGGAGTTCC CGGAAGTGAT 
TACATTAATG CCAATTACAT AGATGGGTAC AGGAAACAGA ATGCCTATAT AGCTACGCAG 
GGCCCACTGC CAGAGACTAT CACTGATTTC TGGAGAATGG TGTGGGAGCA AAGAACCGCC 
ACTATTGTCA TGATGACCCG GCTAGAGGAA AAATCAAGGG TGAAATGTGA CCAGTACTGG 
CCCGGTCGTG GCTCAGAGAC CTATGGTATG ATCCAAGTCA CATTAATGGA CACAGTAGAG 
CTTGCCACCT ACACAGTTAG GACTTTCACA CTTTATAAGA ATGGCTCCAG TGAGAAGCGG 
GATGTCAGAC AGTTCCAATT TATGGCCTGG CCTGATCACG GGGTCCCTGA ATATCCTACT 
CCTATTCTTG GATTCCTTCG CAGAGTGAAA GGCTGTAATC CACCTGATGC TGGTCCTATG 
GTTGTTCATT GCAGTGCTGG TGTGGGCAGA ACAGGATGCT TTATTGTTAT TGATGCCATG 
CTAGAACGGG TGAAACTGGA GAAAACTGTG GATATTTATG GTCATGTGAC CTGCATGCGC 
TCCCAACGAA ATTATATGGT GCAAACAGAG GACCAGTACA TCTTTATCCA TGAGGCTTTG 
CTTGAAGCAG TAATGTGCGG GATCACTGAA GTGCCAGCAC GCAGTCTGTA TGCACATATC 
CAGAAACTGT CTCAGGTCCC TCCGGGTGAG AGTGTTACCT CCATGGAACT AGAGTTCAAG 
CTTTTGGCCA ACTCTAAAGC CCACACCTCA CGATTTATCA GTGCCAACCT CCCATGCAAC 
AAGTTCAAAA ACAGACTGGT AAACATCATG CCATATGAAC TGAGTCGTGT CTCCCTGCAG 
CCCATTCGTG GTGTTGAGGG ATCAGATTAT ATCAATGCAA GTTTCATTGA TGGCTACAGG 
CAGCAGAAAG GCTACATTGC TACTCAGGGT CCATTGGCCG AGACCACTGA GGATTTCTGG 
AGAATGCTGT GGGAACATAA CTCCACCATT GTGGTGATGC TGACAAAGTT GCGAGAAATG 
GGGAGGGAGA AGTGCCATCA GTACTGGCCT GCAGAGCGCT CAGCACGGTA CCAGTATTTT 
GTGGTGGACC CTATGGCTGA GTATAACATG CCTCAGTATA TCCTGCGTGA GTTCAAAGTG 
ACAGATGCAC GGGATGGCCA ATCTCGTACA ATTAGACAAT TCCAGTTCAC TGATTGGCCT 
GAACAAGGAG TGCCAAAAAC AGGTGAAGGC TTTATTGACT TCATTGGGCA GGTGCACAAA 
ACCAAGGAGC AGTTTGGGCA GGATGGGCCA ATCACCGTTC ACTGCAGCGC TGGCGTGGGG 
CGCACTGGAG TATTCATCAC TCTCAGTATT GTACTGGAGC GTATGAGGTA CGAGGGTGTG 
GTTGACATGT TTCAAACAGT GAAGACTTTA AGAACACAGC GGCCAGCAAT GGTACAAACA 
GAGGACCAGT ATCAACTGTG TTATAGAGCC GCACTGGAGT ATCTGGGCAG CTTTGACCAC 
TATGCAACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ptprf
Entrez Gene ID	100493254
Full Name	protein tyrosine phosphatase, receptor type F
Synonyms	LAR,XPTP-LAR
General protein information	Preferred Names protein tyrosine phosphatase, receptor type F Names receptor-type tyrosine-protein phosphatase F
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Rattus norvegicus (Norway rat)	Ptprf	NP_062122.1
Danio rerio (zebrafish)	ptprfb	NP_001077045.1
Danio rerio (zebrafish)	ptprfa	XP_692206.6
	PTPRF	XP_001095521.2
Pan troglodytes (chimpanzee)	PTPRF	XP_001173898.2
Canis lupus familiaris (dog)	PTPRF	XP_005629167.1
Gallus gallus (chicken)	PTPRF	XP_004936750.1
Homo sapiens (human)	PTPRF	NP_002831.2
Mus musculus (house mouse)	Ptprf	NP_035343.2
Bos taurus (cattle)	PTPRF	NP_001094549.1
Xenopus tropicalis (tropical clawed frog)	ptprf	XP_004914019.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ptprf cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

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