PTPRF gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_857873.4	XP_862966.1	receptor-type tyrosine-protein phosphatase F isoform X6
XM_022428059.1	XP_022283767.1	receptor-type tyrosine-protein phosphatase F isoform X7
XM_022428060.1	XP_022283768.1	receptor-type tyrosine-protein phosphatase F isoform X11
XM_005629111.2	XP_005629168.1	receptor-type tyrosine-protein phosphatase F isoform X2
XM_005629110.3	XP_005629167.1	receptor-type tyrosine-protein phosphatase F isoform X1
XM_532613.6	XP_532613.2	receptor-type tyrosine-protein phosphatase F isoform X5
XM_857873.5	XP_862966.1	receptor-type tyrosine-protein phosphatase F isoform X6
XM_022428063.1	XP_022283771.1	receptor-type tyrosine-protein phosphatase F isoform X14
XM_005629113.2	XP_005629170.1	receptor-type tyrosine-protein phosphatase F isoform X4
XM_005629115.2	XP_005629172.1	receptor-type tyrosine-protein phosphatase F isoform X7
XM_005629111.3	XP_005629168.1	receptor-type tyrosine-protein phosphatase F isoform X2
XM_022428062.1	XP_022283770.1	receptor-type tyrosine-protein phosphatase F isoform X13
XM_005629110.2	XP_005629167.1	receptor-type tyrosine-protein phosphatase F isoform X1
XM_005629112.2	XP_005629169.1	receptor-type tyrosine-protein phosphatase F isoform X3
XM_005629117.2	XP_005629174.1	receptor-type tyrosine-protein phosphatase F isoform X9
XM_005629113.3	XP_005629170.1	receptor-type tyrosine-protein phosphatase F isoform X4
XM_005629115.3	XP_005629172.1	receptor-type tyrosine-protein phosphatase F isoform X8
XM_005629117.3	XP_005629174.1	receptor-type tyrosine-protein phosphatase F isoform X10
XM_022428061.1	XP_022283769.1	receptor-type tyrosine-protein phosphatase F isoform X12
XM_532613.5	XP_532613.2	receptor-type tyrosine-protein phosphatase F isoform X5
XM_005629116.2	XP_005629173.1	receptor-type tyrosine-protein phosphatase F isoform X8
XM_005629112.3	XP_005629169.1	receptor-type tyrosine-protein phosphatase F isoform X3
XM_005629116.3	XP_005629173.1	receptor-type tyrosine-protein phosphatase F isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe293526	XM_022428059.1 Latest version!	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe293527	XM_022428060.1 Latest version!	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe293528	XM_022428061.1 Latest version!	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe293529	XM_022428062.1 Latest version!	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe293530	XM_022428063.1 Latest version!	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe17419	XM_005629110.3 Latest version!	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe17420	XM_005629111.3 Latest version!	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe17421	XM_005629112.3 Latest version!	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe17422	XM_005629113.3 Latest version!	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe17423	XM_532613.6 Latest version!	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe17425	XM_857873.5 Latest version!	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe17426	XM_005629115.3 Latest version!	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe17427	XM_005629116.3 Latest version!	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe17428	XM_005629117.3 Latest version!	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OCe17425	XM_857873.4	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe17420	XM_005629111.2	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe17419	XM_005629110.2	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe17422	XM_005629113.2	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe17426	XM_005629115.2	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe17428	XM_005629117.2	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe17423	XM_532613.5	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe17421	XM_005629112.2	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe17427	XM_005629116.2	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

PTPRF ( XM_022428059.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022283767.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe293526
Clone ID Related Accession (Same CDS sequence)	XM_022428059.1
Accession Version	XM_022428059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGG GGAAGAAAGT CAGCTCCCAG CGCTTTGAGG TCATTGAGTT TGATGATGGG 
GCAGGGTCTG TGCTGCGGAT CCAGCCATTG CGGGTGCAAC GAGATGAAGC CATCTACGAG 
TGCACAGCCA CCAACAGTCT GGGAGAGATT AACACGAGTG CCAAGCTCTC AGTTCTCGAA 
GAAGAACAGC TGCCCCCTGG GTTCCCCTCC ATCGACATGG GGCCTCAGCT GAAGGTGGTG 
GAGAAGGCGC GCACAGCCAC CATGCTGTGC GCAGCAGGCG GGAATCCAGA CCCTGAGATC 
TCTTGGTTCA AGGACTTCCT CCCTGTGGAC CCTGCTACAA GCAATGGCCG CATCAAGCAG 
CTACGTTCAG GTGGTTCACC AATCAGAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCACCCCCTC AGCCCCTCCC CAGAAGGTGA CCTGTGTGAG CACGGGCTCC 
ACCACGGTTC GGGTAAGTTG GGTCCCGCCG CCAGCTGCCA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCAGTGGAC GGCGAGGACC GAGGGCGGCA TGTGGTGGAG 
AGCATCAGTC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACTGACGTG GGCCCCGGCC CAGAGAGCAG CCCGGTGCTC 
GTGCGCACAG ACGAGGACGT GCCCAGTGGA CCGCCACGGA AGGTGGAGGT GGAACCACTG 
AATTCCACTG CTGTGCGTGT CTCTTGGAAG CTGCCCGTCC CCAGCAAGCA GCACGGCCAG 
ATTCGCGGCT ACCAGGTCAC CTATGTGCGG CTAGAGAATG GCGAGCCCCG TGGCCCACCC 
ATCATCCAGG ACGTCATGCT GGCTGAGGCC CAGTGGCGGC CAGAGGAGTC CGAGGACTAT 
GAAACCATAA TCAGCGGCCT GACCCCAGAG ACCACCTATT CCATTACCGT CGCCGCCTAC 
ACCACCAAAG GGGATGGTGC CCGCAGCAAG CCCAAAGTTG TCACAACGAC CGGGGCAGTC 
CCAGGCCGGC CCACCATGAT GGTCAGCACC ACGGCCATGA ACACCGCACT GCTGCAGTGG 
CACCCCCCCA AGGAGCTGCC AGGGGAGCTG CTGGGCTACC GGCTACAGTA CCACCGTGCC 
GATGAGGCAA AGCCCAACAT CCTAGATTTT GGCAAGGACG ACCAGCACTT TACAGTCACC 
GGCCTACACA AGGGAGCCAC CTACATTTTC CGGCTTGCCG CCAAGAACCG GGCTGGCCCG 
GGAGAAGAGT TCGAGAAGGA GATCACGACT CCCGAGGATG CGCCCAGCGG CTTCCCCCAA 
AACCTGCGCG TGATAGGGCT GACCACCTCT ACCACGGAGC TGACCTGGGA CCCCCCGGTG 
CTGGCGGAGA GGAACGGGCG CATCACCAAC TACTCCGTGG TGTACCGTGA CATCAACAGC 
CAGCAGGAGC TACAGAACGT CACCGCCGAC ACCCACCTCA TGCTCTCCGG CCTCAAGCCG 
GACACCACTT ACGACATCAA GGTCCGCGCA CGTACCAGCA AGGGCGCCGG CCCACTCAGC 
CCCAGCATCC AGTCCCGGAC CATGCCCATG GAGCAAGTGT TTGCCAAGAA CTTCCGTGTG 
GCGGCCGCTA TGAAGACGTC TGTGCTGCTC AGCTGGGAGG TCCCAGACTC CTACAAGTCG 
GCTGTACCCT TCAAGATCCT GTACAACGGG CAGAGTGTGG AGGTGGACGG GCACTCCATG 
CGGAAACTGA TCGCAGACCT GCAGCCGAAC ACGGAGTACT CGTTCGTGCT GATGAACCGT 
GGCAGCAGCG CGGGGGGCCT GCAGCACCTC GTGTCCATCC GCACGGCCCC AGACCTCCTG 
CCACACAAGC CGATGCCCGC CTCCGCCTTC ATAGAGGATG GTCGCTTCAC CCTCTCCATG 
CCCCACGTGC AGGATGCCTC ACTCATCAGG TGGTTCTACA TCGTGGTGGT ACCCATCGAC 
CGCGTGGGTG GGAGTGTGCT GGCCCCGAGA TGGAGCACGC CTGAGGAGCT GGAGCTGGAC 
GAGCTTCTGG AGGCCATCGA GCAGGGCGGT GGGGAGCGGC GACGACGGCG GCAGGCGGAG 
CGGCTGAAGC CATATGTGGC TGCACAGGTG GACGTGCTCC CCGAGAGCTT CACCCTAGGA 
GACAAGAAGA GCTACCGGGG CTTCTACAAC CGGCCTCTGT CTCCGGACCT GAGCTATCAG 
TGCTTTGTGC TCGCCTCCCT GAAGGAACCC GTGGACCAGA AGCGCTATGC CTCCAGCCCC 
TACTCTGATG AGATCGTGGT CCAAGTGACA CCAGCCCAGC AGCAGGAGGA GCCTGAGATG 
CTGTGGGTGA CAGGCCCCGT CCTCGCAGTC ATCCTCATCA TCCTCATTGT CATCGCCATC 
CTCCTGTTCA AGAGGAAGAG GACTCACTCC CCATCATCCA AGGACGAGCA GTCAATCGGG 
CTGAAGGACT CCTTGCTGGC CCATTCCTCT GACCCTGTGG AGATGCGGAG GCTCAACTAC 
CAGACTCCAG GTTCCAGTGT CCCCAGTTGC CCGAATATCT CAAGTATGCG AGACCACCCG 
CCCATCCCCA TCACTGACCT GGCCGACAAC ATTGAGCGCC TCAAAGCCAA CGACGGCCTC 
AAGTTCTCCC AGGAGTACGA GTCCATTGAC CCTGGACAGC AGTTCACCTG GGAGAATTCA 
AACCTGGAAG TGAACAAGCC CAAGAACCGC TATGCAAACG TCATCGCCTA CGACCACTCC 
CGAGTCATCC TTACCTCCGT CGATGGTGTC CCAGGGAGTG ACTATATCAA CGCCAACTAC 
ATCGATGGCT ACCGCAAGCA GAACGCCTAC ATTGCCACGC AGGGCCCACT GCCCGAGACC 
ATGGGCGATT TCTGGCGGAT GGTGTGGGAA CAGCGCACAG CCACCGTGGT CATGATGACA 
CGGCTGGAGG AGAAGTCTCG GGTGAAGTGT GATCAGTACT GGCCAGCCCG GGGTACCGAG 
ACCCACGGCC TCATTCAGGT GACCCTGCTG GACACGGTGG AGCTGGCCAC ATACACTGTG 
CGCACCTTTG CACTCCACAA GAGTGGCTCC AGCGAGAAGC GGGAGCTGCG TCAGTTCCAG 
TTCATGGCCT GGCCGGACCA CGGGGTTCCC GAGTACCCCA CCCCCATCCT GGCCTTCCTG 
CGGCGGGTCA AGGCCTGTAA CCCGCTAGAC GCAGGGCCCA TGGTGGTGCA CTGCAGCGCG 
GGCGTGGGCC GCACGGGCTG TTTCATCGTC ATCGACGCCA TGCTGGAGCG GATGAAGCAC 
GAGAAGACAG TGGACATCTA TGGCCACGTG ACATGCATGC GATCGCAGCG GAACTACATG 
GTGCAGACAG AGGACCAGTA CGTGTTCATC CACGAGGCGC TGCTAGAGGC CGCCACGTGC 
GGACACACGG AGGTGCCTGC CCGCAGCCTG TATGCCCATA TCCAGAAGCT GGGCCAAGTG 
CCTCCCGGGG AGAGTGTGAC TGCCATGGAG CTCGAGTTCA AGCTGCTGGC CAGCTCCAAG 
GCCCACACAT CCCGCTTCAT TAGCGCCAAC CTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTGAACATCA TGCCCTATGA GTTGACCCGT GTGTGTCTGC AGCCCATCCG TGGTGTGGAG 
GGCTCTGACT ACATCAATGC CAGCTTCTTG GACGGCTATA GGCAGCAGAA GGCCTACATC 
GCCACGCAGG GGCCCCTGGC AGAGAGCACC GAGGACTTCT GGCGCATGCT CTGGGAGCAC 
GGCTCCACCA TCGTCGTCAT GCTGACCAGG CTTCGGGAGA TGGGCAGGGA GAAATGCCAC 
CAGTACTGGC CAGCAGAGCG CTCCGCTCGC TACCAATACT TTGTCGTTGA CCCGATGGCT 
GAGTACAACA TGCCGCAGTA CATCCTGCGT GAGTTCAAGG TCACAGATGC CCGGGATGGG 
CAGTCGAGGA CAATCCGGCA GTTCCAGTTC ACAGACTGGC CCGAGCAGGG CGTGCCTAAG 
ACGGGTGAGG GCTTCATCGA CTTCATCGGG CAGGTGCACA AGACCAAGGA GCAGTTCGGG 
CAGGACGGGC CCATCACGGT GCACTGCAGT GCCGGCGTGG GCCGCACCGG GGTGTTCATC 
ACTCTGAGCA TCGTCCTGGA GCGGATGCGC TACGAGGGCG TGGTCGACAT GTTCCAGACC 
GTGAAGACCC TGCGCACACA GCGCCCAGCC ATGGTGCAGA CGGAGGACCA GTACCAGCTG 
TGCTATCGTG CGGCCCTGGA GTACCTTGGC AGCTTTGACC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022283767.1
CDS	228..5801
Translation	MKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGGSPIRGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPAASRNGVITQYSVAYEAVDGEDRGRHVVESISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQWRPEESEDYETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGSSVPSCPNISSMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_022428059.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022428059.1

ORF Insert Sequence:

ATGAAGAAGG GGAAGAAAGT CAGCTCCCAG CGCTTTGAGG TCATTGAGTT TGATGATGGG 
GCAGGGTCTG TGCTGCGGAT CCAGCCATTG CGGGTGCAAC GAGATGAAGC CATCTACGAG 
TGCACAGCCA CCAACAGTCT GGGAGAGATT AACACGAGTG CCAAGCTCTC AGTTCTCGAA 
GAAGAACAGC TGCCCCCTGG GTTCCCCTCC ATCGACATGG GGCCTCAGCT GAAGGTGGTG 
GAGAAGGCGC GCACAGCCAC CATGCTGTGC GCAGCAGGCG GGAATCCAGA CCCTGAGATC 
TCTTGGTTCA AGGACTTCCT CCCTGTGGAC CCTGCTACAA GCAATGGCCG CATCAAGCAG 
CTACGTTCAG GTGGTTCACC AATCAGAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCACCCCCTC AGCCCCTCCC CAGAAGGTGA CCTGTGTGAG CACGGGCTCC 
ACCACGGTTC GGGTAAGTTG GGTCCCGCCG CCAGCTGCCA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCAGTGGAC GGCGAGGACC GAGGGCGGCA TGTGGTGGAG 
AGCATCAGTC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACTGACGTG GGCCCCGGCC CAGAGAGCAG CCCGGTGCTC 
GTGCGCACAG ACGAGGACGT GCCCAGTGGA CCGCCACGGA AGGTGGAGGT GGAACCACTG 
AATTCCACTG CTGTGCGTGT CTCTTGGAAG CTGCCCGTCC CCAGCAAGCA GCACGGCCAG 
ATTCGCGGCT ACCAGGTCAC CTATGTGCGG CTAGAGAATG GCGAGCCCCG TGGCCCACCC 
ATCATCCAGG ACGTCATGCT GGCTGAGGCC CAGTGGCGGC CAGAGGAGTC CGAGGACTAT 
GAAACCATAA TCAGCGGCCT GACCCCAGAG ACCACCTATT CCATTACCGT CGCCGCCTAC 
ACCACCAAAG GGGATGGTGC CCGCAGCAAG CCCAAAGTTG TCACAACGAC CGGGGCAGTC 
CCAGGCCGGC CCACCATGAT GGTCAGCACC ACGGCCATGA ACACCGCACT GCTGCAGTGG 
CACCCCCCCA AGGAGCTGCC AGGGGAGCTG CTGGGCTACC GGCTACAGTA CCACCGTGCC 
GATGAGGCAA AGCCCAACAT CCTAGATTTT GGCAAGGACG ACCAGCACTT TACAGTCACC 
GGCCTACACA AGGGAGCCAC CTACATTTTC CGGCTTGCCG CCAAGAACCG GGCTGGCCCG 
GGAGAAGAGT TCGAGAAGGA GATCACGACT CCCGAGGATG CGCCCAGCGG CTTCCCCCAA 
AACCTGCGCG TGATAGGGCT GACCACCTCT ACCACGGAGC TGACCTGGGA CCCCCCGGTG 
CTGGCGGAGA GGAACGGGCG CATCACCAAC TACTCCGTGG TGTACCGTGA CATCAACAGC 
CAGCAGGAGC TACAGAACGT CACCGCCGAC ACCCACCTCA TGCTCTCCGG CCTCAAGCCG 
GACACCACTT ACGACATCAA GGTCCGCGCA CGTACCAGCA AGGGCGCCGG CCCACTCAGC 
CCCAGCATCC AGTCCCGGAC CATGCCCATG GAGCAAGTGT TTGCCAAGAA CTTCCGTGTG 
GCGGCCGCTA TGAAGACGTC TGTGCTGCTC AGCTGGGAGG TCCCAGACTC CTACAAGTCG 
GCTGTACCCT TCAAGATCCT GTACAACGGG CAGAGTGTGG AGGTGGACGG GCACTCCATG 
CGGAAACTGA TCGCAGACCT GCAGCCGAAC ACGGAGTACT CGTTCGTGCT GATGAACCGT 
GGCAGCAGCG CGGGGGGCCT GCAGCACCTC GTGTCCATCC GCACGGCCCC AGACCTCCTG 
CCACACAAGC CGATGCCCGC CTCCGCCTTC ATAGAGGATG GTCGCTTCAC CCTCTCCATG 
CCCCACGTGC AGGATGCCTC ACTCATCAGG TGGTTCTACA TCGTGGTGGT ACCCATCGAC 
CGCGTGGGTG GGAGTGTGCT GGCCCCGAGA TGGAGCACGC CTGAGGAGCT GGAGCTGGAC 
GAGCTTCTGG AGGCCATCGA GCAGGGCGGT GGGGAGCGGC GACGACGGCG GCAGGCGGAG 
CGGCTGAAGC CATATGTGGC TGCACAGGTG GACGTGCTCC CCGAGAGCTT CACCCTAGGA 
GACAAGAAGA GCTACCGGGG CTTCTACAAC CGGCCTCTGT CTCCGGACCT GAGCTATCAG 
TGCTTTGTGC TCGCCTCCCT GAAGGAACCC GTGGACCAGA AGCGCTATGC CTCCAGCCCC 
TACTCTGATG AGATCGTGGT CCAAGTGACA CCAGCCCAGC AGCAGGAGGA GCCTGAGATG 
CTGTGGGTGA CAGGCCCCGT CCTCGCAGTC ATCCTCATCA TCCTCATTGT CATCGCCATC 
CTCCTGTTCA AGAGGAAGAG GACTCACTCC CCATCATCCA AGGACGAGCA GTCAATCGGG 
CTGAAGGACT CCTTGCTGGC CCATTCCTCT GACCCTGTGG AGATGCGGAG GCTCAACTAC 
CAGACTCCAG GTTCCAGTGT CCCCAGTTGC CCGAATATCT CAAGTATGCG AGACCACCCG 
CCCATCCCCA TCACTGACCT GGCCGACAAC ATTGAGCGCC TCAAAGCCAA CGACGGCCTC 
AAGTTCTCCC AGGAGTACGA GTCCATTGAC CCTGGACAGC AGTTCACCTG GGAGAATTCA 
AACCTGGAAG TGAACAAGCC CAAGAACCGC TATGCAAACG TCATCGCCTA CGACCACTCC 
CGAGTCATCC TTACCTCCGT CGATGGTGTC CCAGGGAGTG ACTATATCAA CGCCAACTAC 
ATCGATGGCT ACCGCAAGCA GAACGCCTAC ATTGCCACGC AGGGCCCACT GCCCGAGACC 
ATGGGCGATT TCTGGCGGAT GGTGTGGGAA CAGCGCACAG CCACCGTGGT CATGATGACA 
CGGCTGGAGG AGAAGTCTCG GGTGAAGTGT GATCAGTACT GGCCAGCCCG GGGTACCGAG 
ACCCACGGCC TCATTCAGGT GACCCTGCTG GACACGGTGG AGCTGGCCAC ATACACTGTG 
CGCACCTTTG CACTCCACAA GAGTGGCTCC AGCGAGAAGC GGGAGCTGCG TCAGTTCCAG 
TTCATGGCCT GGCCGGACCA CGGGGTTCCC GAGTACCCCA CCCCCATCCT GGCCTTCCTG 
CGGCGGGTCA AGGCCTGTAA CCCGCTAGAC GCAGGGCCCA TGGTGGTGCA CTGCAGCGCG 
GGCGTGGGCC GCACGGGCTG TTTCATCGTC ATCGACGCCA TGCTGGAGCG GATGAAGCAC 
GAGAAGACAG TGGACATCTA TGGCCACGTG ACATGCATGC GATCGCAGCG GAACTACATG 
GTGCAGACAG AGGACCAGTA CGTGTTCATC CACGAGGCGC TGCTAGAGGC CGCCACGTGC 
GGACACACGG AGGTGCCTGC CCGCAGCCTG TATGCCCATA TCCAGAAGCT GGGCCAAGTG 
CCTCCCGGGG AGAGTGTGAC TGCCATGGAG CTCGAGTTCA AGCTGCTGGC CAGCTCCAAG 
GCCCACACAT CCCGCTTCAT TAGCGCCAAC CTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTGAACATCA TGCCCTATGA GTTGACCCGT GTGTGTCTGC AGCCCATCCG TGGTGTGGAG 
GGCTCTGACT ACATCAATGC CAGCTTCTTG GACGGCTATA GGCAGCAGAA GGCCTACATC 
GCCACGCAGG GGCCCCTGGC AGAGAGCACC GAGGACTTCT GGCGCATGCT CTGGGAGCAC 
GGCTCCACCA TCGTCGTCAT GCTGACCAGG CTTCGGGAGA TGGGCAGGGA GAAATGCCAC 
CAGTACTGGC CAGCAGAGCG CTCCGCTCGC TACCAATACT TTGTCGTTGA CCCGATGGCT 
GAGTACAACA TGCCGCAGTA CATCCTGCGT GAGTTCAAGG TCACAGATGC CCGGGATGGG 
CAGTCGAGGA CAATCCGGCA GTTCCAGTTC ACAGACTGGC CCGAGCAGGG CGTGCCTAAG 
ACGGGTGAGG GCTTCATCGA CTTCATCGGG CAGGTGCACA AGACCAAGGA GCAGTTCGGG 
CAGGACGGGC CCATCACGGT GCACTGCAGT GCCGGCGTGG GCCGCACCGG GGTGTTCATC 
ACTCTGAGCA TCGTCCTGGA GCGGATGCGC TACGAGGGCG TGGTCGACAT GTTCCAGACC 
GTGAAGACCC TGCGCACACA GCGCCCAGCC ATGGTGCAGA CGGAGGACCA GTACCAGCTG 
TGCTATCGTG CGGCCCTGGA GTACCTTGGC AGCTTTGACC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_022428060.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022283768.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X11, mRNA.

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CloneID	OCe293527
Clone ID Related Accession (Same CDS sequence)	XM_022428060.1
Accession Version	XM_022428060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GGAAACCATA 
ATCAGCGGCC TGACCCCAGA GACCACCTAT TCCATTACCG TCGCCGCCTA CACCACCAAA 
GGGGATGGTG CCCGCAGCAA GCCCAAAGTT GTCACAACGA CCGGGGCAGT GTTTGCCAAG 
AACTTCCGTG TGGCGGCCGC TATGAAGACG TCTGTGCTGC TCAGCTGGGA GGTCCCAGAC 
TCCTACAAGT CGGCTGTACC CTTCAAGATC CTGTACAACG GGCAGAGTGT GGAGGTGGAC 
GGGCACTCCA TGCGGAAACT GATCGCAGAC CTGCAGCCGA ACACGGAGTA CTCGTTCGTG 
CTGATGAACC GTGGCAGCAG CGCGGGGGGC CTGCAGCACC TCGTGTCCAT CCGCACGGCC 
CCAGACCTCC TGCCACACAA GCCGATGCCC GCCTCCGCCT TCATAGAGGA TGGTCGCTTC 
ACCCTCTCCA TGCCCCACGT GCAGGATGCC TCACTCATCA GGTGGTTCTA CATCGTGGTG 
GTACCCATCG ACCGCGTGGG TGGGAGTGTG CTGGCCCCGA GATGGAGCAC GCCTGAGGAG 
CTGGAGCTGG ACGAGCTTCT GGAGGCCATC GAGCAGGGCG GTGGGGAGCG GCGACGACGG 
CGGCAGGCGG AGCGGCTGAA GCCATATGTG GCTGCACAGG TGGACGTGCT CCCCGAGAGC 
TTCACCCTAG GAGACAAGAA GAGCTACCGG GGCTTCTACA ACCGGCCTCT GTCTCCGGAC 
CTGAGCTATC AGTGCTTTGT GCTCGCCTCC CTGAAGGAAC CCGTGGACCA GAAGCGCTAT 
GCCTCCAGCC CCTACTCTGA TGAGATCGTG GTCCAAGTGA CACCAGCCCA GCAGCAGGAG 
GAGCCTGAGA TGCTGTGGGT GACAGGCCCC GTCCTCGCAG TCATCCTCAT CATCCTCATT 
GTCATCGCCA TCCTCCTGTT CAAGAGGAAG AGGACTCACT CCCCATCATC CAAGGACGAG 
CAGTCAATCG GGCTGAAGGA CTCCTTGCTG GCCCATTCCT CTGACCCTGT GGAGATGCGG 
AGGCTCAACT ACCAGACTCC AGGTTCCAGT GTCCCCAGTT GCCCGAATAT CTCAAGTATG 
CGAGACCACC CGCCCATCCC CATCACTGAC CTGGCCGACA ACATTGAGCG CCTCAAAGCC 
AACGACGGCC TCAAGTTCTC CCAGGAGTAC GAGTCCATTG ACCCTGGACA GCAGTTCACC 
TGGGAGAATT CAAACCTGGA AGTGAACAAG CCCAAGAACC GCTATGCAAA CGTCATCGCC 
TACGACCACT CCCGAGTCAT CCTTACCTCC GTCGATGGTG TCCCAGGGAG TGACTATATC 
AACGCCAACT ACATCGATGG CTACCGCAAG CAGAACGCCT ACATTGCCAC GCAGGGCCCA 
CTGCCCGAGA CCATGGGCGA TTTCTGGCGG ATGGTGTGGG AACAGCGCAC AGCCACCGTG 
GTCATGATGA CACGGCTGGA GGAGAAGTCT CGGGTGAAGT GTGATCAGTA CTGGCCAGCC 
CGGGGTACCG AGACCCACGG CCTCATTCAG GTGACCCTGC TGGACACGGT GGAGCTGGCC 
ACATACACTG TGCGCACCTT TGCACTCCAC AAGAGTGGCT CCAGCGAGAA GCGGGAGCTG 
CGTCAGTTCC AGTTCATGGC CTGGCCGGAC CACGGGGTTC CCGAGTACCC CACCCCCATC 
CTGGCCTTCC TGCGGCGGGT CAAGGCCTGT AACCCGCTAG ACGCAGGGCC CATGGTGGTG 
CACTGCAGCG CGGGCGTGGG CCGCACGGGC TGTTTCATCG TCATCGACGC CATGCTGGAG 
CGGATGAAGC ACGAGAAGAC AGTGGACATC TATGGCCACG TGACATGCAT GCGATCGCAG 
CGGAACTACA TGGTGCAGAC AGAGGACCAG TACGTGTTCA TCCACGAGGC GCTGCTAGAG 
GCCGCCACGT GCGGACACAC GGAGGTGCCT GCCCGCAGCC TGTATGCCCA TATCCAGAAG 
CTGGGCCAAG TGCCTCCCGG GGAGAGTGTG ACTGCCATGG AGCTCGAGTT CAAGCTGCTG 
GCCAGCTCCA AGGCCCACAC ATCCCGCTTC ATTAGCGCCA ACCTGCCCTG CAACAAGTTC 
AAGAACCGCC TGGTGAACAT CATGCCCTAT GAGTTGACCC GTGTGTGTCT GCAGCCCATC 
CGTGGTGTGG AGGGCTCTGA CTACATCAAT GCCAGCTTCT TGGACGGCTA TAGGCAGCAG 
AAGGCCTACA TCGCCACGCA GGGGCCCCTG GCAGAGAGCA CCGAGGACTT CTGGCGCATG 
CTCTGGGAGC ACGGCTCCAC CATCGTCGTC ATGCTGACCA GGCTTCGGGA GATGGGCAGG 
GAGAAATGCC ACCAGTACTG GCCAGCAGAG CGCTCCGCTC GCTACCAATA CTTTGTCGTT 
GACCCGATGG CTGAGTACAA CATGCCGCAG TACATCCTGC GTGAGTTCAA GGTCACAGAT 
GCCCGGGATG GGCAGTCGAG GACAATCCGG CAGTTCCAGT TCACAGACTG GCCCGAGCAG 
GGCGTGCCTA AGACGGGTGA GGGCTTCATC GACTTCATCG GGCAGGTGCA CAAGACCAAG 
GAGCAGTTCG GGCAGGACGG GCCCATCACG GTGCACTGCA GTGCCGGCGT GGGCCGCACC 
GGGGTGTTCA TCACTCTGAG CATCGTCCTG GAGCGGATGC GCTACGAGGG CGTGGTCGAC 
ATGTTCCAGA CCGTGAAGAC CCTGCGCACA CAGCGCCCAG CCATGGTGCA GACGGAGGAC 
CAGTACCAGC TGTGCTATCG TGCGGCCCTG GAGTACCTTG GCAGCTTTGA CCACTATGCA 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022283768.1
CDS	125..5290
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGGSPIRGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPAASRNGVITQYSVAYEAVDGEDRGRHVVESISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGSSVPSCPNISSMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_022428060.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022428060.1

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GGAAACCATA 
ATCAGCGGCC TGACCCCAGA GACCACCTAT TCCATTACCG TCGCCGCCTA CACCACCAAA 
GGGGATGGTG CCCGCAGCAA GCCCAAAGTT GTCACAACGA CCGGGGCAGT GTTTGCCAAG 
AACTTCCGTG TGGCGGCCGC TATGAAGACG TCTGTGCTGC TCAGCTGGGA GGTCCCAGAC 
TCCTACAAGT CGGCTGTACC CTTCAAGATC CTGTACAACG GGCAGAGTGT GGAGGTGGAC 
GGGCACTCCA TGCGGAAACT GATCGCAGAC CTGCAGCCGA ACACGGAGTA CTCGTTCGTG 
CTGATGAACC GTGGCAGCAG CGCGGGGGGC CTGCAGCACC TCGTGTCCAT CCGCACGGCC 
CCAGACCTCC TGCCACACAA GCCGATGCCC GCCTCCGCCT TCATAGAGGA TGGTCGCTTC 
ACCCTCTCCA TGCCCCACGT GCAGGATGCC TCACTCATCA GGTGGTTCTA CATCGTGGTG 
GTACCCATCG ACCGCGTGGG TGGGAGTGTG CTGGCCCCGA GATGGAGCAC GCCTGAGGAG 
CTGGAGCTGG ACGAGCTTCT GGAGGCCATC GAGCAGGGCG GTGGGGAGCG GCGACGACGG 
CGGCAGGCGG AGCGGCTGAA GCCATATGTG GCTGCACAGG TGGACGTGCT CCCCGAGAGC 
TTCACCCTAG GAGACAAGAA GAGCTACCGG GGCTTCTACA ACCGGCCTCT GTCTCCGGAC 
CTGAGCTATC AGTGCTTTGT GCTCGCCTCC CTGAAGGAAC CCGTGGACCA GAAGCGCTAT 
GCCTCCAGCC CCTACTCTGA TGAGATCGTG GTCCAAGTGA CACCAGCCCA GCAGCAGGAG 
GAGCCTGAGA TGCTGTGGGT GACAGGCCCC GTCCTCGCAG TCATCCTCAT CATCCTCATT 
GTCATCGCCA TCCTCCTGTT CAAGAGGAAG AGGACTCACT CCCCATCATC CAAGGACGAG 
CAGTCAATCG GGCTGAAGGA CTCCTTGCTG GCCCATTCCT CTGACCCTGT GGAGATGCGG 
AGGCTCAACT ACCAGACTCC AGGTTCCAGT GTCCCCAGTT GCCCGAATAT CTCAAGTATG 
CGAGACCACC CGCCCATCCC CATCACTGAC CTGGCCGACA ACATTGAGCG CCTCAAAGCC 
AACGACGGCC TCAAGTTCTC CCAGGAGTAC GAGTCCATTG ACCCTGGACA GCAGTTCACC 
TGGGAGAATT CAAACCTGGA AGTGAACAAG CCCAAGAACC GCTATGCAAA CGTCATCGCC 
TACGACCACT CCCGAGTCAT CCTTACCTCC GTCGATGGTG TCCCAGGGAG TGACTATATC 
AACGCCAACT ACATCGATGG CTACCGCAAG CAGAACGCCT ACATTGCCAC GCAGGGCCCA 
CTGCCCGAGA CCATGGGCGA TTTCTGGCGG ATGGTGTGGG AACAGCGCAC AGCCACCGTG 
GTCATGATGA CACGGCTGGA GGAGAAGTCT CGGGTGAAGT GTGATCAGTA CTGGCCAGCC 
CGGGGTACCG AGACCCACGG CCTCATTCAG GTGACCCTGC TGGACACGGT GGAGCTGGCC 
ACATACACTG TGCGCACCTT TGCACTCCAC AAGAGTGGCT CCAGCGAGAA GCGGGAGCTG 
CGTCAGTTCC AGTTCATGGC CTGGCCGGAC CACGGGGTTC CCGAGTACCC CACCCCCATC 
CTGGCCTTCC TGCGGCGGGT CAAGGCCTGT AACCCGCTAG ACGCAGGGCC CATGGTGGTG 
CACTGCAGCG CGGGCGTGGG CCGCACGGGC TGTTTCATCG TCATCGACGC CATGCTGGAG 
CGGATGAAGC ACGAGAAGAC AGTGGACATC TATGGCCACG TGACATGCAT GCGATCGCAG 
CGGAACTACA TGGTGCAGAC AGAGGACCAG TACGTGTTCA TCCACGAGGC GCTGCTAGAG 
GCCGCCACGT GCGGACACAC GGAGGTGCCT GCCCGCAGCC TGTATGCCCA TATCCAGAAG 
CTGGGCCAAG TGCCTCCCGG GGAGAGTGTG ACTGCCATGG AGCTCGAGTT CAAGCTGCTG 
GCCAGCTCCA AGGCCCACAC ATCCCGCTTC ATTAGCGCCA ACCTGCCCTG CAACAAGTTC 
AAGAACCGCC TGGTGAACAT CATGCCCTAT GAGTTGACCC GTGTGTGTCT GCAGCCCATC 
CGTGGTGTGG AGGGCTCTGA CTACATCAAT GCCAGCTTCT TGGACGGCTA TAGGCAGCAG 
AAGGCCTACA TCGCCACGCA GGGGCCCCTG GCAGAGAGCA CCGAGGACTT CTGGCGCATG 
CTCTGGGAGC ACGGCTCCAC CATCGTCGTC ATGCTGACCA GGCTTCGGGA GATGGGCAGG 
GAGAAATGCC ACCAGTACTG GCCAGCAGAG CGCTCCGCTC GCTACCAATA CTTTGTCGTT 
GACCCGATGG CTGAGTACAA CATGCCGCAG TACATCCTGC GTGAGTTCAA GGTCACAGAT 
GCCCGGGATG GGCAGTCGAG GACAATCCGG CAGTTCCAGT TCACAGACTG GCCCGAGCAG 
GGCGTGCCTA AGACGGGTGA GGGCTTCATC GACTTCATCG GGCAGGTGCA CAAGACCAAG 
GAGCAGTTCG GGCAGGACGG GCCCATCACG GTGCACTGCA GTGCCGGCGT GGGCCGCACC 
GGGGTGTTCA TCACTCTGAG CATCGTCCTG GAGCGGATGC GCTACGAGGG CGTGGTCGAC 
ATGTTCCAGA CCGTGAAGAC CCTGCGCACA CAGCGCCCAG CCATGGTGCA GACGGAGGAC 
CAGTACCAGC TGTGCTATCG TGCGGCCCTG GAGTACCTTG GCAGCTTTGA CCACTATGCA 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_022428061.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022283769.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCe293528
Clone ID Related Accession (Same CDS sequence)	XM_022428061.1
Accession Version	XM_022428061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ATGCCCAGTG GACCGCCACG GAAGGTGGAG 
GTGGAACCAC TGAATTCCAC TGCTGTGCGT GTCTCTTGGA AGCTGCCCGT CCCCAGCAAG 
CAGCACGGCC AGATTCGCGG CTACCAGGTC ACCTATGTGC GGCTAGAGAA TGGCGAGCCC 
CGTGGCCCAC CCATCATCCA GGACGTCATG CTGGCTGAGG CCCAGTGGCG GCCAGAGGAG 
TCCGAGGACT ATGAAACCAT AATCAGCGGC CTGACCCCAG AGACCACCTA TTCCATTACC 
GTCGCCGCCT ACACCACCAA AGGGGATGGT GCCCGCAGCA AGCCCAAAGT TGTCACAACG 
ACCGGGGCAG TGTTTGCCAA GAACTTCCGT GTGGCGGCCG CTATGAAGAC GTCTGTGCTG 
CTCAGCTGGG AGGTCCCAGA CTCCTACAAG TCGGCTGTAC CCTTCAAGAT CCTGTACAAC 
GGGCAGAGTG TGGAGGTGGA CGGGCACTCC ATGCGGAAAC TGATCGCAGA CCTGCAGCCG 
AACACGGAGT ACTCGTTCGT GCTGATGAAC CGTGGCAGCA GCGCGGGGGG CCTGCAGCAC 
CTCGTGTCCA TCCGCACGGC CCCAGACCTC CTGCCACACA AGCCGATGCC CGCCTCCGCC 
TTCATAGAGG ATGGTCGCTT CACCCTCTCC ATGCCCCACG TGCAGGATGC CTCACTCATC 
AGGTGGTTCT ACATCGTGGT GGTACCCATC GACCGCGTGG GTGGGAGTGT GCTGGCCCCG 
AGATGGAGCA CGCCTGAGGA GCTGGAGCTG GACGAGCTTC TGGAGGCCAT CGAGCAGGGC 
GGTGGGGAGC GGCGACGACG GCGGCAGGCG GAGCGGCTGA AGCCATATGT GGCTGCACAG 
GTGGACGTGC TCCCCGAGAG CTTCACCCTA GGAGACAAGA AGAGCTACCG GGGCTTCTAC 
AACCGGCCTC TGTCTCCGGA CCTGAGCTAT CAGTGCTTTG TGCTCGCCTC CCTGAAGGAA 
CCCGTGGACC AGAAGCGCTA TGCCTCCAGC CCCTACTCTG ATGAGATCGT GGTCCAAGTG 
ACACCAGCCC AGCAGCAGGA GGAGCCTGAG ATGCTGTGGG TGACAGGCCC CGTCCTCGCA 
GTCATCCTCA TCATCCTCAT TGTCATCGCC ATCCTCCTGT TCAAGAGGAA GAGGACTCAC 
TCCCCATCAT CCAAGGACGA GCAGTCAATC GGGCTGAAGG ACTCCTTGCT GGCCCATTCC 
TCTGACCCTG TGGAGATGCG GAGGCTCAAC TACCAGACTC CAGGTTCCAG TGTCCCCAGT 
TGCCCGAATA TCTCAAGTAT GCGAGACCAC CCGCCCATCC CCATCACTGA CCTGGCCGAC 
AACATTGAGC GCCTCAAAGC CAACGACGGC CTCAAGTTCT CCCAGGAGTA CGAGTCCATT 
GACCCTGGAC AGCAGTTCAC CTGGGAGAAT TCAAACCTGG AAGTGAACAA GCCCAAGAAC 
CGCTATGCAA ACGTCATCGC CTACGACCAC TCCCGAGTCA TCCTTACCTC CGTCGATGGT 
GTCCCAGGGA GTGACTATAT CAACGCCAAC TACATCGATG GCTACCGCAA GCAGAACGCC 
TACATTGCCA CGCAGGGCCC ACTGCCCGAG ACCATGGGCG ATTTCTGGCG GATGGTGTGG 
GAACAGCGCA CAGCCACCGT GGTCATGATG ACACGGCTGG AGGAGAAGTC TCGGGTGAAG 
TGTGATCAGT ACTGGCCAGC CCGGGGTACC GAGACCCACG GCCTCATTCA GGTGACCCTG 
CTGGACACGG TGGAGCTGGC CACATACACT GTGCGCACCT TTGCACTCCA CAAGAGTGGC 
TCCAGCGAGA AGCGGGAGCT GCGTCAGTTC CAGTTCATGG CCTGGCCGGA CCACGGGGTT 
CCCGAGTACC CCACCCCCAT CCTGGCCTTC CTGCGGCGGG TCAAGGCCTG TAACCCGCTA 
GACGCAGGGC CCATGGTGGT GCACTGCAGC GCGGGCGTGG GCCGCACGGG CTGTTTCATC 
GTCATCGACG CCATGCTGGA GCGGATGAAG CACGAGAAGA CAGTGGACAT CTATGGCCAC 
GTGACATGCA TGCGATCGCA GCGGAACTAC ATGGTGCAGA CAGAGGACCA GTACGTGTTC 
ATCCACGAGG CGCTGCTAGA GGCCGCCACG TGCGGACACA CGGAGGTGCC TGCCCGCAGC 
CTGTATGCCC ATATCCAGAA GCTGGGCCAA GTGCCTCCCG GGGAGAGTGT GACTGCCATG 
GAGCTCGAGT TCAAGCTGCT GGCCAGCTCC AAGGCCCACA CATCCCGCTT CATTAGCGCC 
AACCTGCCCT GCAACAAGTT CAAGAACCGC CTGGTGAACA TCATGCCCTA TGAGTTGACC 
CGTGTGTGTC TGCAGCCCAT CCGTGGTGTG GAGGGCTCTG ACTACATCAA TGCCAGCTTC 
TTGGACGGCT ATAGGCAGCA GAAGGCCTAC ATCGCCACGC AGGGGCCCCT GGCAGAGAGC 
ACCGAGGACT TCTGGCGCAT GCTCTGGGAG CACGGCTCCA CCATCGTCGT CATGCTGACC 
AGGCTTCGGG AGATGGGCAG GGAGAAATGC CACCAGTACT GGCCAGCAGA GCGCTCCGCT 
CGCTACCAAT ACTTTGTCGT TGACCCGATG GCTGAGTACA ACATGCCGCA GTACATCCTG 
CGTGAGTTCA AGGTCACAGA TGCCCGGGAT GGGCAGTCGA GGACAATCCG GCAGTTCCAG 
TTCACAGACT GGCCCGAGCA GGGCGTGCCT AAGACGGGTG AGGGCTTCAT CGACTTCATC 
GGGCAGGTGC ACAAGACCAA GGAGCAGTTC GGGCAGGACG GGCCCATCAC GGTGCACTGC 
AGTGCCGGCG TGGGCCGCAC CGGGGTGTTC ATCACTCTGA GCATCGTCCT GGAGCGGATG 
CGCTACGAGG GCGTGGTCGA CATGTTCCAG ACCGTGAAGA CCCTGCGCAC ACAGCGCCCA 
GCCATGGTGC AGACGGAGGA CCAGTACCAG CTGTGCTATC GTGCGGCCCT GGAGTACCTT 
GGCAGCTTTG ACCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022283769.1
CDS	125..5011
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGGSPIRGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSMPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQWRPEESEDYETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGSSVPSCPNISSMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_022428061.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022428061.1

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ATGCCCAGTG GACCGCCACG GAAGGTGGAG 
GTGGAACCAC TGAATTCCAC TGCTGTGCGT GTCTCTTGGA AGCTGCCCGT CCCCAGCAAG 
CAGCACGGCC AGATTCGCGG CTACCAGGTC ACCTATGTGC GGCTAGAGAA TGGCGAGCCC 
CGTGGCCCAC CCATCATCCA GGACGTCATG CTGGCTGAGG CCCAGTGGCG GCCAGAGGAG 
TCCGAGGACT ATGAAACCAT AATCAGCGGC CTGACCCCAG AGACCACCTA TTCCATTACC 
GTCGCCGCCT ACACCACCAA AGGGGATGGT GCCCGCAGCA AGCCCAAAGT TGTCACAACG 
ACCGGGGCAG TGTTTGCCAA GAACTTCCGT GTGGCGGCCG CTATGAAGAC GTCTGTGCTG 
CTCAGCTGGG AGGTCCCAGA CTCCTACAAG TCGGCTGTAC CCTTCAAGAT CCTGTACAAC 
GGGCAGAGTG TGGAGGTGGA CGGGCACTCC ATGCGGAAAC TGATCGCAGA CCTGCAGCCG 
AACACGGAGT ACTCGTTCGT GCTGATGAAC CGTGGCAGCA GCGCGGGGGG CCTGCAGCAC 
CTCGTGTCCA TCCGCACGGC CCCAGACCTC CTGCCACACA AGCCGATGCC CGCCTCCGCC 
TTCATAGAGG ATGGTCGCTT CACCCTCTCC ATGCCCCACG TGCAGGATGC CTCACTCATC 
AGGTGGTTCT ACATCGTGGT GGTACCCATC GACCGCGTGG GTGGGAGTGT GCTGGCCCCG 
AGATGGAGCA CGCCTGAGGA GCTGGAGCTG GACGAGCTTC TGGAGGCCAT CGAGCAGGGC 
GGTGGGGAGC GGCGACGACG GCGGCAGGCG GAGCGGCTGA AGCCATATGT GGCTGCACAG 
GTGGACGTGC TCCCCGAGAG CTTCACCCTA GGAGACAAGA AGAGCTACCG GGGCTTCTAC 
AACCGGCCTC TGTCTCCGGA CCTGAGCTAT CAGTGCTTTG TGCTCGCCTC CCTGAAGGAA 
CCCGTGGACC AGAAGCGCTA TGCCTCCAGC CCCTACTCTG ATGAGATCGT GGTCCAAGTG 
ACACCAGCCC AGCAGCAGGA GGAGCCTGAG ATGCTGTGGG TGACAGGCCC CGTCCTCGCA 
GTCATCCTCA TCATCCTCAT TGTCATCGCC ATCCTCCTGT TCAAGAGGAA GAGGACTCAC 
TCCCCATCAT CCAAGGACGA GCAGTCAATC GGGCTGAAGG ACTCCTTGCT GGCCCATTCC 
TCTGACCCTG TGGAGATGCG GAGGCTCAAC TACCAGACTC CAGGTTCCAG TGTCCCCAGT 
TGCCCGAATA TCTCAAGTAT GCGAGACCAC CCGCCCATCC CCATCACTGA CCTGGCCGAC 
AACATTGAGC GCCTCAAAGC CAACGACGGC CTCAAGTTCT CCCAGGAGTA CGAGTCCATT 
GACCCTGGAC AGCAGTTCAC CTGGGAGAAT TCAAACCTGG AAGTGAACAA GCCCAAGAAC 
CGCTATGCAA ACGTCATCGC CTACGACCAC TCCCGAGTCA TCCTTACCTC CGTCGATGGT 
GTCCCAGGGA GTGACTATAT CAACGCCAAC TACATCGATG GCTACCGCAA GCAGAACGCC 
TACATTGCCA CGCAGGGCCC ACTGCCCGAG ACCATGGGCG ATTTCTGGCG GATGGTGTGG 
GAACAGCGCA CAGCCACCGT GGTCATGATG ACACGGCTGG AGGAGAAGTC TCGGGTGAAG 
TGTGATCAGT ACTGGCCAGC CCGGGGTACC GAGACCCACG GCCTCATTCA GGTGACCCTG 
CTGGACACGG TGGAGCTGGC CACATACACT GTGCGCACCT TTGCACTCCA CAAGAGTGGC 
TCCAGCGAGA AGCGGGAGCT GCGTCAGTTC CAGTTCATGG CCTGGCCGGA CCACGGGGTT 
CCCGAGTACC CCACCCCCAT CCTGGCCTTC CTGCGGCGGG TCAAGGCCTG TAACCCGCTA 
GACGCAGGGC CCATGGTGGT GCACTGCAGC GCGGGCGTGG GCCGCACGGG CTGTTTCATC 
GTCATCGACG CCATGCTGGA GCGGATGAAG CACGAGAAGA CAGTGGACAT CTATGGCCAC 
GTGACATGCA TGCGATCGCA GCGGAACTAC ATGGTGCAGA CAGAGGACCA GTACGTGTTC 
ATCCACGAGG CGCTGCTAGA GGCCGCCACG TGCGGACACA CGGAGGTGCC TGCCCGCAGC 
CTGTATGCCC ATATCCAGAA GCTGGGCCAA GTGCCTCCCG GGGAGAGTGT GACTGCCATG 
GAGCTCGAGT TCAAGCTGCT GGCCAGCTCC AAGGCCCACA CATCCCGCTT CATTAGCGCC 
AACCTGCCCT GCAACAAGTT CAAGAACCGC CTGGTGAACA TCATGCCCTA TGAGTTGACC 
CGTGTGTGTC TGCAGCCCAT CCGTGGTGTG GAGGGCTCTG ACTACATCAA TGCCAGCTTC 
TTGGACGGCT ATAGGCAGCA GAAGGCCTAC ATCGCCACGC AGGGGCCCCT GGCAGAGAGC 
ACCGAGGACT TCTGGCGCAT GCTCTGGGAG CACGGCTCCA CCATCGTCGT CATGCTGACC 
AGGCTTCGGG AGATGGGCAG GGAGAAATGC CACCAGTACT GGCCAGCAGA GCGCTCCGCT 
CGCTACCAAT ACTTTGTCGT TGACCCGATG GCTGAGTACA ACATGCCGCA GTACATCCTG 
CGTGAGTTCA AGGTCACAGA TGCCCGGGAT GGGCAGTCGA GGACAATCCG GCAGTTCCAG 
TTCACAGACT GGCCCGAGCA GGGCGTGCCT AAGACGGGTG AGGGCTTCAT CGACTTCATC 
GGGCAGGTGC ACAAGACCAA GGAGCAGTTC GGGCAGGACG GGCCCATCAC GGTGCACTGC 
AGTGCCGGCG TGGGCCGCAC CGGGGTGTTC ATCACTCTGA GCATCGTCCT GGAGCGGATG 
CGCTACGAGG GCGTGGTCGA CATGTTCCAG ACCGTGAAGA CCCTGCGCAC ACAGCGCCCA 
GCCATGGTGC AGACGGAGGA CCAGTACCAG CTGTGCTATC GTGCGGCCCT GGAGTACCTT 
GGCAGCTTTG ACCACTATGC AACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_022428062.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022283770.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OCe293529
Clone ID Related Accession (Same CDS sequence)	XM_022428062.1
Accession Version	XM_022428062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCATGCCCAG TGGACCGCCA CGGAAGGTGG AGGTGGAACC ACTGAATTCC 
ACTGCTGTGC GTGTCTCTTG GAAGCTGCCC GTCCCCAGCA AGCAGCACGG CCAGATTCGC 
GGCTACCAGG TCACCTATGT GCGGCTAGAG AATGGCGAGC CCCGTGGCCC ACCCATCATC 
CAGGACGTCA TGCTGGCTGA GGCCCAGTGG CGGCCAGAGG AGTCCGAGGA CTATGAAACC 
ATAATCAGCG GCCTGACCCC AGAGACCACC TATTCCATTA CCGTCGCCGC CTACACCACC 
AAAGGGGATG GTGCCCGCAG CAAGCCCAAA GTTGTCACAA CGACCGGGGC AGTGTTTGCC 
AAGAACTTCC GTGTGGCGGC CGCTATGAAG ACGTCTGTGC TGCTCAGCTG GGAGGTCCCA 
GACTCCTACA AGTCGGCTGT ACCCTTCAAG ATCCTGTACA ACGGGCAGAG TGTGGAGGTG 
GACGGGCACT CCATGCGGAA ACTGATCGCA GACCTGCAGC CGAACACGGA GTACTCGTTC 
GTGCTGATGA ACCGTGGCAG CAGCGCGGGG GGCCTGCAGC ACCTCGTGTC CATCCGCACG 
GCCCCAGACC TCCTGCCACA CAAGCCGATG CCCGCCTCCG CCTTCATAGA GGATGGTCGC 
TTCACCCTCT CCATGCCCCA CGTGCAGGAT GCCTCACTCA TCAGGTGGTT CTACATCGTG 
GTGGTACCCA TCGACCGCGT GGGTGGGAGT GTGCTGGCCC CGAGATGGAG CACGCCTGAG 
GAGCTGGAGC TGGACGAGCT TCTGGAGGCC ATCGAGCAGG GCGGTGGGGA GCGGCGACGA 
CGGCGGCAGG CGGAGCGGCT GAAGCCATAT GTGGCTGCAC AGGTGGACGT GCTCCCCGAG 
AGCTTCACCC TAGGAGACAA GAAGAGCTAC CGGGGCTTCT ACAACCGGCC TCTGTCTCCG 
GACCTGAGCT ATCAGTGCTT TGTGCTCGCC TCCCTGAAGG AACCCGTGGA CCAGAAGCGC 
TATGCCTCCA GCCCCTACTC TGATGAGATC GTGGTCCAAG TGACACCAGC CCAGCAGCAG 
GAGGAGCCTG AGATGCTGTG GGTGACAGGC CCCGTCCTCG CAGTCATCCT CATCATCCTC 
ATTGTCATCG CCATCCTCCT GTTCAAGAGG AAGAGGACTC ACTCCCCATC ATCCAAGGAC 
GAGCAGTCAA TCGGGCTGAA GGACTCCTTG CTGGCCCATT CCTCTGACCC TGTGGAGATG 
CGGAGGCTCA ACTACCAGAC TCCAGGTTCC AGTGTCCCCA GTTGCCCGAA TATCTCAAGT 
ATGCGAGACC ACCCGCCCAT CCCCATCACT GACCTGGCCG ACAACATTGA GCGCCTCAAA 
GCCAACGACG GCCTCAAGTT CTCCCAGGAG TACGAGTCCA TTGACCCTGG ACAGCAGTTC 
ACCTGGGAGA ATTCAAACCT GGAAGTGAAC AAGCCCAAGA ACCGCTATGC AAACGTCATC 
GCCTACGACC ACTCCCGAGT CATCCTTACC TCCGTCGATG GTGTCCCAGG GAGTGACTAT 
ATCAACGCCA ACTACATCGA TGGCTACCGC AAGCAGAACG CCTACATTGC CACGCAGGGC 
CCACTGCCCG AGACCATGGG CGATTTCTGG CGGATGGTGT GGGAACAGCG CACAGCCACC 
GTGGTCATGA TGACACGGCT GGAGGAGAAG TCTCGGGTGA AGTGTGATCA GTACTGGCCA 
GCCCGGGGTA CCGAGACCCA CGGCCTCATT CAGGTGACCC TGCTGGACAC GGTGGAGCTG 
GCCACATACA CTGTGCGCAC CTTTGCACTC CACAAGAGTG GCTCCAGCGA GAAGCGGGAG 
CTGCGTCAGT TCCAGTTCAT GGCCTGGCCG GACCACGGGG TTCCCGAGTA CCCCACCCCC 
ATCCTGGCCT TCCTGCGGCG GGTCAAGGCC TGTAACCCGC TAGACGCAGG GCCCATGGTG 
GTGCACTGCA GCGCGGGCGT GGGCCGCACG GGCTGTTTCA TCGTCATCGA CGCCATGCTG 
GAGCGGATGA AGCACGAGAA GACAGTGGAC ATCTATGGCC ACGTGACATG CATGCGATCG 
CAGCGGAACT ACATGGTGCA GACAGAGGAC CAGTACGTGT TCATCCACGA GGCGCTGCTA 
GAGGCCGCCA CGTGCGGACA CACGGAGGTG CCTGCCCGCA GCCTGTATGC CCATATCCAG 
AAGCTGGGCC AAGTGCCTCC CGGGGAGAGT GTGACTGCCA TGGAGCTCGA GTTCAAGCTG 
CTGGCCAGCT CCAAGGCCCA CACATCCCGC TTCATTAGCG CCAACCTGCC CTGCAACAAG 
TTCAAGAACC GCCTGGTGAA CATCATGCCC TATGAGTTGA CCCGTGTGTG TCTGCAGCCC 
ATCCGTGGTG TGGAGGGCTC TGACTACATC AATGCCAGCT TCTTGGACGG CTATAGGCAG 
CAGAAGGCCT ACATCGCCAC GCAGGGGCCC CTGGCAGAGA GCACCGAGGA CTTCTGGCGC 
ATGCTCTGGG AGCACGGCTC CACCATCGTC GTCATGCTGA CCAGGCTTCG GGAGATGGGC 
AGGGAGAAAT GCCACCAGTA CTGGCCAGCA GAGCGCTCCG CTCGCTACCA ATACTTTGTC 
GTTGACCCGA TGGCTGAGTA CAACATGCCG CAGTACATCC TGCGTGAGTT CAAGGTCACA 
GATGCCCGGG ATGGGCAGTC GAGGACAATC CGGCAGTTCC AGTTCACAGA CTGGCCCGAG 
CAGGGCGTGC CTAAGACGGG TGAGGGCTTC ATCGACTTCA TCGGGCAGGT GCACAAGACC 
AAGGAGCAGT TCGGGCAGGA CGGGCCCATC ACGGTGCACT GCAGTGCCGG CGTGGGCCGC 
ACCGGGGTGT TCATCACTCT GAGCATCGTC CTGGAGCGGA TGCGCTACGA GGGCGTGGTC 
GACATGTTCC AGACCGTGAA GACCCTGCGC ACACAGCGCC CAGCCATGGT GCAGACGGAG 
GACCAGTACC AGCTGTGCTA TCGTGCGGCC CTGGAGTACC TTGGCAGCTT TGACCACTAT 
GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022283770.1
CDS	125..4993
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSMPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQWRPEESEDYETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGSSVPSCPNISSMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_022428062.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022428062.1

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCATGCCCAG TGGACCGCCA CGGAAGGTGG AGGTGGAACC ACTGAATTCC 
ACTGCTGTGC GTGTCTCTTG GAAGCTGCCC GTCCCCAGCA AGCAGCACGG CCAGATTCGC 
GGCTACCAGG TCACCTATGT GCGGCTAGAG AATGGCGAGC CCCGTGGCCC ACCCATCATC 
CAGGACGTCA TGCTGGCTGA GGCCCAGTGG CGGCCAGAGG AGTCCGAGGA CTATGAAACC 
ATAATCAGCG GCCTGACCCC AGAGACCACC TATTCCATTA CCGTCGCCGC CTACACCACC 
AAAGGGGATG GTGCCCGCAG CAAGCCCAAA GTTGTCACAA CGACCGGGGC AGTGTTTGCC 
AAGAACTTCC GTGTGGCGGC CGCTATGAAG ACGTCTGTGC TGCTCAGCTG GGAGGTCCCA 
GACTCCTACA AGTCGGCTGT ACCCTTCAAG ATCCTGTACA ACGGGCAGAG TGTGGAGGTG 
GACGGGCACT CCATGCGGAA ACTGATCGCA GACCTGCAGC CGAACACGGA GTACTCGTTC 
GTGCTGATGA ACCGTGGCAG CAGCGCGGGG GGCCTGCAGC ACCTCGTGTC CATCCGCACG 
GCCCCAGACC TCCTGCCACA CAAGCCGATG CCCGCCTCCG CCTTCATAGA GGATGGTCGC 
TTCACCCTCT CCATGCCCCA CGTGCAGGAT GCCTCACTCA TCAGGTGGTT CTACATCGTG 
GTGGTACCCA TCGACCGCGT GGGTGGGAGT GTGCTGGCCC CGAGATGGAG CACGCCTGAG 
GAGCTGGAGC TGGACGAGCT TCTGGAGGCC ATCGAGCAGG GCGGTGGGGA GCGGCGACGA 
CGGCGGCAGG CGGAGCGGCT GAAGCCATAT GTGGCTGCAC AGGTGGACGT GCTCCCCGAG 
AGCTTCACCC TAGGAGACAA GAAGAGCTAC CGGGGCTTCT ACAACCGGCC TCTGTCTCCG 
GACCTGAGCT ATCAGTGCTT TGTGCTCGCC TCCCTGAAGG AACCCGTGGA CCAGAAGCGC 
TATGCCTCCA GCCCCTACTC TGATGAGATC GTGGTCCAAG TGACACCAGC CCAGCAGCAG 
GAGGAGCCTG AGATGCTGTG GGTGACAGGC CCCGTCCTCG CAGTCATCCT CATCATCCTC 
ATTGTCATCG CCATCCTCCT GTTCAAGAGG AAGAGGACTC ACTCCCCATC ATCCAAGGAC 
GAGCAGTCAA TCGGGCTGAA GGACTCCTTG CTGGCCCATT CCTCTGACCC TGTGGAGATG 
CGGAGGCTCA ACTACCAGAC TCCAGGTTCC AGTGTCCCCA GTTGCCCGAA TATCTCAAGT 
ATGCGAGACC ACCCGCCCAT CCCCATCACT GACCTGGCCG ACAACATTGA GCGCCTCAAA 
GCCAACGACG GCCTCAAGTT CTCCCAGGAG TACGAGTCCA TTGACCCTGG ACAGCAGTTC 
ACCTGGGAGA ATTCAAACCT GGAAGTGAAC AAGCCCAAGA ACCGCTATGC AAACGTCATC 
GCCTACGACC ACTCCCGAGT CATCCTTACC TCCGTCGATG GTGTCCCAGG GAGTGACTAT 
ATCAACGCCA ACTACATCGA TGGCTACCGC AAGCAGAACG CCTACATTGC CACGCAGGGC 
CCACTGCCCG AGACCATGGG CGATTTCTGG CGGATGGTGT GGGAACAGCG CACAGCCACC 
GTGGTCATGA TGACACGGCT GGAGGAGAAG TCTCGGGTGA AGTGTGATCA GTACTGGCCA 
GCCCGGGGTA CCGAGACCCA CGGCCTCATT CAGGTGACCC TGCTGGACAC GGTGGAGCTG 
GCCACATACA CTGTGCGCAC CTTTGCACTC CACAAGAGTG GCTCCAGCGA GAAGCGGGAG 
CTGCGTCAGT TCCAGTTCAT GGCCTGGCCG GACCACGGGG TTCCCGAGTA CCCCACCCCC 
ATCCTGGCCT TCCTGCGGCG GGTCAAGGCC TGTAACCCGC TAGACGCAGG GCCCATGGTG 
GTGCACTGCA GCGCGGGCGT GGGCCGCACG GGCTGTTTCA TCGTCATCGA CGCCATGCTG 
GAGCGGATGA AGCACGAGAA GACAGTGGAC ATCTATGGCC ACGTGACATG CATGCGATCG 
CAGCGGAACT ACATGGTGCA GACAGAGGAC CAGTACGTGT TCATCCACGA GGCGCTGCTA 
GAGGCCGCCA CGTGCGGACA CACGGAGGTG CCTGCCCGCA GCCTGTATGC CCATATCCAG 
AAGCTGGGCC AAGTGCCTCC CGGGGAGAGT GTGACTGCCA TGGAGCTCGA GTTCAAGCTG 
CTGGCCAGCT CCAAGGCCCA CACATCCCGC TTCATTAGCG CCAACCTGCC CTGCAACAAG 
TTCAAGAACC GCCTGGTGAA CATCATGCCC TATGAGTTGA CCCGTGTGTG TCTGCAGCCC 
ATCCGTGGTG TGGAGGGCTC TGACTACATC AATGCCAGCT TCTTGGACGG CTATAGGCAG 
CAGAAGGCCT ACATCGCCAC GCAGGGGCCC CTGGCAGAGA GCACCGAGGA CTTCTGGCGC 
ATGCTCTGGG AGCACGGCTC CACCATCGTC GTCATGCTGA CCAGGCTTCG GGAGATGGGC 
AGGGAGAAAT GCCACCAGTA CTGGCCAGCA GAGCGCTCCG CTCGCTACCA ATACTTTGTC 
GTTGACCCGA TGGCTGAGTA CAACATGCCG CAGTACATCC TGCGTGAGTT CAAGGTCACA 
GATGCCCGGG ATGGGCAGTC GAGGACAATC CGGCAGTTCC AGTTCACAGA CTGGCCCGAG 
CAGGGCGTGC CTAAGACGGG TGAGGGCTTC ATCGACTTCA TCGGGCAGGT GCACAAGACC 
AAGGAGCAGT TCGGGCAGGA CGGGCCCATC ACGGTGCACT GCAGTGCCGG CGTGGGCCGC 
ACCGGGGTGT TCATCACTCT GAGCATCGTC CTGGAGCGGA TGCGCTACGA GGGCGTGGTC 
GACATGTTCC AGACCGTGAA GACCCTGCGC ACACAGCGCC CAGCCATGGT GCAGACGGAG 
GACCAGTACC AGCTGTGCTA TCGTGCGGCC CTGGAGTACC TTGGCAGCTT TGACCACTAT 
GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_022428063.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022283771.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X14, mRNA.

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CloneID	OCe293530
Clone ID Related Accession (Same CDS sequence)	XM_022428063.1
Accession Version	XM_022428063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCATGCCCAG TGGACCGCCA CGGAAGGTGG AGGTGGAACC ACTGAATTCC 
ACTGCTGTGC GTGTCTCTTG GAAGCTGCCC GTCCCCAGCA AGCAGCACGG CCAGATTCGC 
GGCTACCAGG TCACCTATGT GCGGCTAGAG AATGGCGAGC CCCGTGGCCC ACCCATCATC 
CAGGACGTCA TGCTGGCTGA GGCCCAGGAA ACCATAATCA GCGGCCTGAC CCCAGAGACC 
ACCTATTCCA TTACCGTCGC CGCCTACACC ACCAAAGGGG ATGGTGCCCG CAGCAAGCCC 
AAAGTTGTCA CAACGACCGG GGCAGTGTTT GCCAAGAACT TCCGTGTGGC GGCCGCTATG 
AAGACGTCTG TGCTGCTCAG CTGGGAGGTC CCAGACTCCT ACAAGTCGGC TGTACCCTTC 
AAGATCCTGT ACAACGGGCA GAGTGTGGAG GTGGACGGGC ACTCCATGCG GAAACTGATC 
GCAGACCTGC AGCCGAACAC GGAGTACTCG TTCGTGCTGA TGAACCGTGG CAGCAGCGCG 
GGGGGCCTGC AGCACCTCGT GTCCATCCGC ACGGCCCCAG ACCTCCTGCC ACACAAGCCG 
ATGCCCGCCT CCGCCTTCAT AGAGGATGGT CGCTTCACCC TCTCCATGCC CCACGTGCAG 
GATGCCTCAC TCATCAGGTG GTTCTACATC GTGGTGGTAC CCATCGACCG CGTGGGTGGG 
AGTGTGCTGG CCCCGAGATG GAGCACGCCT GAGGAGCTGG AGCTGGACGA GCTTCTGGAG 
GCCATCGAGC AGGGCGGTGG GGAGCGGCGA CGACGGCGGC AGGCGGAGCG GCTGAAGCCA 
TATGTGGCTG CACAGGTGGA CGTGCTCCCC GAGAGCTTCA CCCTAGGAGA CAAGAAGAGC 
TACCGGGGCT TCTACAACCG GCCTCTGTCT CCGGACCTGA GCTATCAGTG CTTTGTGCTC 
GCCTCCCTGA AGGAACCCGT GGACCAGAAG CGCTATGCCT CCAGCCCCTA CTCTGATGAG 
ATCGTGGTCC AAGTGACACC AGCCCAGCAG CAGGAGGAGC CTGAGATGCT GTGGGTGACA 
GGCCCCGTCC TCGCAGTCAT CCTCATCATC CTCATTGTCA TCGCCATCCT CCTGTTCAAG 
AGGAAGAGGA CTCACTCCCC ATCATCCAAG GACGAGCAGT CAATCGGGCT GAAGGACTCC 
TTGCTGGCCC ATTCCTCTGA CCCTGTGGAG ATGCGGAGGC TCAACTACCA GACTCCAGGT 
ATGCGAGACC ACCCGCCCAT CCCCATCACT GACCTGGCCG ACAACATTGA GCGCCTCAAA 
GCCAACGACG GCCTCAAGTT CTCCCAGGAG TACGAGTCCA TTGACCCTGG ACAGCAGTTC 
ACCTGGGAGA ATTCAAACCT GGAAGTGAAC AAGCCCAAGA ACCGCTATGC AAACGTCATC 
GCCTACGACC ACTCCCGAGT CATCCTTACC TCCGTCGATG GTGTCCCAGG GAGTGACTAT 
ATCAACGCCA ACTACATCGA TGGCTACCGC AAGCAGAACG CCTACATTGC CACGCAGGGC 
CCACTGCCCG AGACCATGGG CGATTTCTGG CGGATGGTGT GGGAACAGCG CACAGCCACC 
GTGGTCATGA TGACACGGCT GGAGGAGAAG TCTCGGGTGA AGTGTGATCA GTACTGGCCA 
GCCCGGGGTA CCGAGACCCA CGGCCTCATT CAGGTGACCC TGCTGGACAC GGTGGAGCTG 
GCCACATACA CTGTGCGCAC CTTTGCACTC CACAAGAGTG GCTCCAGCGA GAAGCGGGAG 
CTGCGTCAGT TCCAGTTCAT GGCCTGGCCG GACCACGGGG TTCCCGAGTA CCCCACCCCC 
ATCCTGGCCT TCCTGCGGCG GGTCAAGGCC TGTAACCCGC TAGACGCAGG GCCCATGGTG 
GTGCACTGCA GCGCGGGCGT GGGCCGCACG GGCTGTTTCA TCGTCATCGA CGCCATGCTG 
GAGCGGATGA AGCACGAGAA GACAGTGGAC ATCTATGGCC ACGTGACATG CATGCGATCG 
CAGCGGAACT ACATGGTGCA GACAGAGGAC CAGTACGTGT TCATCCACGA GGCGCTGCTA 
GAGGCCGCCA CGTGCGGACA CACGGAGGTG CCTGCCCGCA GCCTGTATGC CCATATCCAG 
AAGCTGGGCC AAGTGCCTCC CGGGGAGAGT GTGACTGCCA TGGAGCTCGA GTTCAAGCTG 
CTGGCCAGCT CCAAGGCCCA CACATCCCGC TTCATTAGCG CCAACCTGCC CTGCAACAAG 
TTCAAGAACC GCCTGGTGAA CATCATGCCC TATGAGTTGA CCCGTGTGTG TCTGCAGCCC 
ATCCGTGGTG TGGAGGGCTC TGACTACATC AATGCCAGCT TCTTGGACGG CTATAGGCAG 
CAGAAGGCCT ACATCGCCAC GCAGGGGCCC CTGGCAGAGA GCACCGAGGA CTTCTGGCGC 
ATGCTCTGGG AGCACGGCTC CACCATCGTC GTCATGCTGA CCAGGCTTCG GGAGATGGGC 
AGGGAGAAAT GCCACCAGTA CTGGCCAGCA GAGCGCTCCG CTCGCTACCA ATACTTTGTC 
GTTGACCCGA TGGCTGAGTA CAACATGCCG CAGTACATCC TGCGTGAGTT CAAGGTCACA 
GATGCCCGGG ATGGGCAGTC GAGGACAATC CGGCAGTTCC AGTTCACAGA CTGGCCCGAG 
CAGGGCGTGC CTAAGACGGG TGAGGGCTTC ATCGACTTCA TCGGGCAGGT GCACAAGACC 
AAGGAGCAGT TCGGGCAGGA CGGGCCCATC ACGGTGCACT GCAGTGCCGG CGTGGGCCGC 
ACCGGGGTGT TCATCACTCT GAGCATCGTC CTGGAGCGGA TGCGCTACGA GGGCGTGGTC 
GACATGTTCC AGACCGTGAA GACCCTGCGC ACACAGCGCC CAGCCATGGT GCAGACGGAG 
GACCAGTACC AGCTGTGCTA TCGTGCGGCC CTGGAGTACC TTGGCAGCTT TGACCACTAT 
GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022283771.1
CDS	125..4933
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSMPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_022428063.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022428063.1

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCATGCCCAG TGGACCGCCA CGGAAGGTGG AGGTGGAACC ACTGAATTCC 
ACTGCTGTGC GTGTCTCTTG GAAGCTGCCC GTCCCCAGCA AGCAGCACGG CCAGATTCGC 
GGCTACCAGG TCACCTATGT GCGGCTAGAG AATGGCGAGC CCCGTGGCCC ACCCATCATC 
CAGGACGTCA TGCTGGCTGA GGCCCAGGAA ACCATAATCA GCGGCCTGAC CCCAGAGACC 
ACCTATTCCA TTACCGTCGC CGCCTACACC ACCAAAGGGG ATGGTGCCCG CAGCAAGCCC 
AAAGTTGTCA CAACGACCGG GGCAGTGTTT GCCAAGAACT TCCGTGTGGC GGCCGCTATG 
AAGACGTCTG TGCTGCTCAG CTGGGAGGTC CCAGACTCCT ACAAGTCGGC TGTACCCTTC 
AAGATCCTGT ACAACGGGCA GAGTGTGGAG GTGGACGGGC ACTCCATGCG GAAACTGATC 
GCAGACCTGC AGCCGAACAC GGAGTACTCG TTCGTGCTGA TGAACCGTGG CAGCAGCGCG 
GGGGGCCTGC AGCACCTCGT GTCCATCCGC ACGGCCCCAG ACCTCCTGCC ACACAAGCCG 
ATGCCCGCCT CCGCCTTCAT AGAGGATGGT CGCTTCACCC TCTCCATGCC CCACGTGCAG 
GATGCCTCAC TCATCAGGTG GTTCTACATC GTGGTGGTAC CCATCGACCG CGTGGGTGGG 
AGTGTGCTGG CCCCGAGATG GAGCACGCCT GAGGAGCTGG AGCTGGACGA GCTTCTGGAG 
GCCATCGAGC AGGGCGGTGG GGAGCGGCGA CGACGGCGGC AGGCGGAGCG GCTGAAGCCA 
TATGTGGCTG CACAGGTGGA CGTGCTCCCC GAGAGCTTCA CCCTAGGAGA CAAGAAGAGC 
TACCGGGGCT TCTACAACCG GCCTCTGTCT CCGGACCTGA GCTATCAGTG CTTTGTGCTC 
GCCTCCCTGA AGGAACCCGT GGACCAGAAG CGCTATGCCT CCAGCCCCTA CTCTGATGAG 
ATCGTGGTCC AAGTGACACC AGCCCAGCAG CAGGAGGAGC CTGAGATGCT GTGGGTGACA 
GGCCCCGTCC TCGCAGTCAT CCTCATCATC CTCATTGTCA TCGCCATCCT CCTGTTCAAG 
AGGAAGAGGA CTCACTCCCC ATCATCCAAG GACGAGCAGT CAATCGGGCT GAAGGACTCC 
TTGCTGGCCC ATTCCTCTGA CCCTGTGGAG ATGCGGAGGC TCAACTACCA GACTCCAGGT 
ATGCGAGACC ACCCGCCCAT CCCCATCACT GACCTGGCCG ACAACATTGA GCGCCTCAAA 
GCCAACGACG GCCTCAAGTT CTCCCAGGAG TACGAGTCCA TTGACCCTGG ACAGCAGTTC 
ACCTGGGAGA ATTCAAACCT GGAAGTGAAC AAGCCCAAGA ACCGCTATGC AAACGTCATC 
GCCTACGACC ACTCCCGAGT CATCCTTACC TCCGTCGATG GTGTCCCAGG GAGTGACTAT 
ATCAACGCCA ACTACATCGA TGGCTACCGC AAGCAGAACG CCTACATTGC CACGCAGGGC 
CCACTGCCCG AGACCATGGG CGATTTCTGG CGGATGGTGT GGGAACAGCG CACAGCCACC 
GTGGTCATGA TGACACGGCT GGAGGAGAAG TCTCGGGTGA AGTGTGATCA GTACTGGCCA 
GCCCGGGGTA CCGAGACCCA CGGCCTCATT CAGGTGACCC TGCTGGACAC GGTGGAGCTG 
GCCACATACA CTGTGCGCAC CTTTGCACTC CACAAGAGTG GCTCCAGCGA GAAGCGGGAG 
CTGCGTCAGT TCCAGTTCAT GGCCTGGCCG GACCACGGGG TTCCCGAGTA CCCCACCCCC 
ATCCTGGCCT TCCTGCGGCG GGTCAAGGCC TGTAACCCGC TAGACGCAGG GCCCATGGTG 
GTGCACTGCA GCGCGGGCGT GGGCCGCACG GGCTGTTTCA TCGTCATCGA CGCCATGCTG 
GAGCGGATGA AGCACGAGAA GACAGTGGAC ATCTATGGCC ACGTGACATG CATGCGATCG 
CAGCGGAACT ACATGGTGCA GACAGAGGAC CAGTACGTGT TCATCCACGA GGCGCTGCTA 
GAGGCCGCCA CGTGCGGACA CACGGAGGTG CCTGCCCGCA GCCTGTATGC CCATATCCAG 
AAGCTGGGCC AAGTGCCTCC CGGGGAGAGT GTGACTGCCA TGGAGCTCGA GTTCAAGCTG 
CTGGCCAGCT CCAAGGCCCA CACATCCCGC TTCATTAGCG CCAACCTGCC CTGCAACAAG 
TTCAAGAACC GCCTGGTGAA CATCATGCCC TATGAGTTGA CCCGTGTGTG TCTGCAGCCC 
ATCCGTGGTG TGGAGGGCTC TGACTACATC AATGCCAGCT TCTTGGACGG CTATAGGCAG 
CAGAAGGCCT ACATCGCCAC GCAGGGGCCC CTGGCAGAGA GCACCGAGGA CTTCTGGCGC 
ATGCTCTGGG AGCACGGCTC CACCATCGTC GTCATGCTGA CCAGGCTTCG GGAGATGGGC 
AGGGAGAAAT GCCACCAGTA CTGGCCAGCA GAGCGCTCCG CTCGCTACCA ATACTTTGTC 
GTTGACCCGA TGGCTGAGTA CAACATGCCG CAGTACATCC TGCGTGAGTT CAAGGTCACA 
GATGCCCGGG ATGGGCAGTC GAGGACAATC CGGCAGTTCC AGTTCACAGA CTGGCCCGAG 
CAGGGCGTGC CTAAGACGGG TGAGGGCTTC ATCGACTTCA TCGGGCAGGT GCACAAGACC 
AAGGAGCAGT TCGGGCAGGA CGGGCCCATC ACGGTGCACT GCAGTGCCGG CGTGGGCCGC 
ACCGGGGTGT TCATCACTCT GAGCATCGTC CTGGAGCGGA TGCGCTACGA GGGCGTGGTC 
GACATGTTCC AGACCGTGAA GACCCTGCGC ACACAGCGCC CAGCCATGGT GCAGACGGAG 
GACCAGTACC AGCTGTGCTA TCGTGCGGCC CTGGAGTACC TTGGCAGCTT TGACCACTAT 
GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_005629110.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629167.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X1, mRNA.

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CloneID	OCe17419
Clone ID Related Accession (Same CDS sequence)	XM_005629110.3 , XM_005629110.2
Accession Version	XM_005629110.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629110.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GTGGCGGCCA 
GAGGAGTCCG AGGACTATGA AACCATAATC AGCGGCCTGA CCCCAGAGAC CACCTATTCC 
ATTACCGTCG CCGCCTACAC CACCAAAGGG GATGGTGCCC GCAGCAAGCC CAAAGTTGTC 
ACAACGACCG GGGCAGTCCC AGGCCGGCCC ACCATGATGG TCAGCACCAC GGCCATGAAC 
ACCGCACTGC TGCAGTGGCA CCCCCCCAAG GAGCTGCCAG GGGAGCTGCT GGGCTACCGG 
CTACAGTACC ACCGTGCCGA TGAGGCAAAG CCCAACATCC TAGATTTTGG CAAGGACGAC 
CAGCACTTTA CAGTCACCGG CCTACACAAG GGAGCCACCT ACATTTTCCG GCTTGCCGCC 
AAGAACCGGG CTGGCCCGGG AGAAGAGTTC GAGAAGGAGA TCACGACTCC CGAGGATGCG 
CCCAGCGGCT TCCCCCAAAA CCTGCGCGTG ATAGGGCTGA CCACCTCTAC CACGGAGCTG 
ACCTGGGACC CCCCGGTGCT GGCGGAGAGG AACGGGCGCA TCACCAACTA CTCCGTGGTG 
TACCGTGACA TCAACAGCCA GCAGGAGCTA CAGAACGTCA CCGCCGACAC CCACCTCATG 
CTCTCCGGCC TCAAGCCGGA CACCACTTAC GACATCAAGG TCCGCGCACG TACCAGCAAG 
GGCGCCGGCC CACTCAGCCC CAGCATCCAG TCCCGGACCA TGCCCATGGA GCAAGTGTTT 
GCCAAGAACT TCCGTGTGGC GGCCGCTATG AAGACGTCTG TGCTGCTCAG CTGGGAGGTC 
CCAGACTCCT ACAAGTCGGC TGTACCCTTC AAGATCCTGT ACAACGGGCA GAGTGTGGAG 
GTGGACGGGC ACTCCATGCG GAAACTGATC GCAGACCTGC AGCCGAACAC GGAGTACTCG 
TTCGTGCTGA TGAACCGTGG CAGCAGCGCG GGGGGCCTGC AGCACCTCGT GTCCATCCGC 
ACGGCCCCAG ACCTCCTGCC ACACAAGCCG ATGCCCGCCT CCGCCTTCAT AGAGGATGGT 
CGCTTCACCC TCTCCATGCC CCACGTGCAG GATGCCTCAC TCATCAGGTG GTTCTACATC 
GTGGTGGTAC CCATCGACCG CGTGGGTGGG AGTGTGCTGG CCCCGAGATG GAGCACGCCT 
GAGGAGCTGG AGCTGGACGA GCTTCTGGAG GCCATCGAGC AGGGCGGTGG GGAGCGGCGA 
CGACGGCGGC AGGCGGAGCG GCTGAAGCCA TATGTGGCTG CACAGGTGGA CGTGCTCCCC 
GAGAGCTTCA CCCTAGGAGA CAAGAAGAGC TACCGGGGCT TCTACAACCG GCCTCTGTCT 
CCGGACCTGA GCTATCAGTG CTTTGTGCTC GCCTCCCTGA AGGAACCCGT GGACCAGAAG 
CGCTATGCCT CCAGCCCCTA CTCTGATGAG ATCGTGGTCC AAGTGACACC AGCCCAGCAG 
CAGGAGGAGC CTGAGATGCT GTGGGTGACA GGCCCCGTCC TCGCAGTCAT CCTCATCATC 
CTCATTGTCA TCGCCATCCT CCTGTTCAAG AGGAAGAGGA CTCACTCCCC ATCATCCAAG 
GACGAGCAGT CAATCGGGCT GAAGGACTCC TTGCTGGCCC ATTCCTCTGA CCCTGTGGAG 
ATGCGGAGGC TCAACTACCA GACTCCAGGT TCCAGTGTCC CCAGTTGCCC GAATATCTCA 
AGTATGCGAG ACCACCCGCC CATCCCCATC ACTGACCTGG CCGACAACAT TGAGCGCCTC 
AAAGCCAACG ACGGCCTCAA GTTCTCCCAG GAGTACGAGT CCATTGACCC TGGACAGCAG 
TTCACCTGGG AGAATTCAAA CCTGGAAGTG AACAAGCCCA AGAACCGCTA TGCAAACGTC 
ATCGCCTACG ACCACTCCCG AGTCATCCTT ACCTCCGTCG ATGGTGTCCC AGGGAGTGAC 
TATATCAACG CCAACTACAT CGATGGCTAC CGCAAGCAGA ACGCCTACAT TGCCACGCAG 
GGCCCACTGC CCGAGACCAT GGGCGATTTC TGGCGGATGG TGTGGGAACA GCGCACAGCC 
ACCGTGGTCA TGATGACACG GCTGGAGGAG AAGTCTCGGG TGAAGTGTGA TCAGTACTGG 
CCAGCCCGGG GTACCGAGAC CCACGGCCTC ATTCAGGTGA CCCTGCTGGA CACGGTGGAG 
CTGGCCACAT ACACTGTGCG CACCTTTGCA CTCCACAAGA GTGGCTCCAG CGAGAAGCGG 
GAGCTGCGTC AGTTCCAGTT CATGGCCTGG CCGGACCACG GGGTTCCCGA GTACCCCACC 
CCCATCCTGG CCTTCCTGCG GCGGGTCAAG GCCTGTAACC CGCTAGACGC AGGGCCCATG 
GTGGTGCACT GCAGCGCGGG CGTGGGCCGC ACGGGCTGTT TCATCGTCAT CGACGCCATG 
CTGGAGCGGA TGAAGCACGA GAAGACAGTG GACATCTATG GCCACGTGAC ATGCATGCGA 
TCGCAGCGGA ACTACATGGT GCAGACAGAG GACCAGTACG TGTTCATCCA CGAGGCGCTG 
CTAGAGGCCG CCACGTGCGG ACACACGGAG GTGCCTGCCC GCAGCCTGTA TGCCCATATC 
CAGAAGCTGG GCCAAGTGCC TCCCGGGGAG AGTGTGACTG CCATGGAGCT CGAGTTCAAG 
CTGCTGGCCA GCTCCAAGGC CCACACATCC CGCTTCATTA GCGCCAACCT GCCCTGCAAC 
AAGTTCAAGA ACCGCCTGGT GAACATCATG CCCTATGAGT TGACCCGTGT GTGTCTGCAG 
CCCATCCGTG GTGTGGAGGG CTCTGACTAC ATCAATGCCA GCTTCTTGGA CGGCTATAGG 
CAGCAGAAGG CCTACATCGC CACGCAGGGG CCCCTGGCAG AGAGCACCGA GGACTTCTGG 
CGCATGCTCT GGGAGCACGG CTCCACCATC GTCGTCATGC TGACCAGGCT TCGGGAGATG 
GGCAGGGAGA AATGCCACCA GTACTGGCCA GCAGAGCGCT CCGCTCGCTA CCAATACTTT 
GTCGTTGACC CGATGGCTGA GTACAACATG CCGCAGTACA TCCTGCGTGA GTTCAAGGTC 
ACAGATGCCC GGGATGGGCA GTCGAGGACA ATCCGGCAGT TCCAGTTCAC AGACTGGCCC 
GAGCAGGGCG TGCCTAAGAC GGGTGAGGGC TTCATCGACT TCATCGGGCA GGTGCACAAG 
ACCAAGGAGC AGTTCGGGCA GGACGGGCCC ATCACGGTGC ACTGCAGTGC CGGCGTGGGC 
CGCACCGGGG TGTTCATCAC TCTGAGCATC GTCCTGGAGC GGATGCGCTA CGAGGGCGTG 
GTCGACATGT TCCAGACCGT GAAGACCCTG CGCACACAGC GCCCAGCCAT GGTGCAGACG 
GAGGACCAGT ACCAGCTGTG CTATCGTGCG GCCCTGGAGT ACCTTGGCAG CTTTGACCAC 
TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629167.1
CDS	125..5896
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGGSPIRGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPAASRNGVITQYSVAYEAVDGEDRGRHVVESISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQWRPEESEDYETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGSSVPSCPNISSMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_005629110.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629110.3

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GTGGCGGCCA 
GAGGAGTCCG AGGACTATGA AACCATAATC AGCGGCCTGA CCCCAGAGAC CACCTATTCC 
ATTACCGTCG CCGCCTACAC CACCAAAGGG GATGGTGCCC GCAGCAAGCC CAAAGTTGTC 
ACAACGACCG GGGCAGTCCC AGGCCGGCCC ACCATGATGG TCAGCACCAC GGCCATGAAC 
ACCGCACTGC TGCAGTGGCA CCCCCCCAAG GAGCTGCCAG GGGAGCTGCT GGGCTACCGG 
CTACAGTACC ACCGTGCCGA TGAGGCAAAG CCCAACATCC TAGATTTTGG CAAGGACGAC 
CAGCACTTTA CAGTCACCGG CCTACACAAG GGAGCCACCT ACATTTTCCG GCTTGCCGCC 
AAGAACCGGG CTGGCCCGGG AGAAGAGTTC GAGAAGGAGA TCACGACTCC CGAGGATGCG 
CCCAGCGGCT TCCCCCAAAA CCTGCGCGTG ATAGGGCTGA CCACCTCTAC CACGGAGCTG 
ACCTGGGACC CCCCGGTGCT GGCGGAGAGG AACGGGCGCA TCACCAACTA CTCCGTGGTG 
TACCGTGACA TCAACAGCCA GCAGGAGCTA CAGAACGTCA CCGCCGACAC CCACCTCATG 
CTCTCCGGCC TCAAGCCGGA CACCACTTAC GACATCAAGG TCCGCGCACG TACCAGCAAG 
GGCGCCGGCC CACTCAGCCC CAGCATCCAG TCCCGGACCA TGCCCATGGA GCAAGTGTTT 
GCCAAGAACT TCCGTGTGGC GGCCGCTATG AAGACGTCTG TGCTGCTCAG CTGGGAGGTC 
CCAGACTCCT ACAAGTCGGC TGTACCCTTC AAGATCCTGT ACAACGGGCA GAGTGTGGAG 
GTGGACGGGC ACTCCATGCG GAAACTGATC GCAGACCTGC AGCCGAACAC GGAGTACTCG 
TTCGTGCTGA TGAACCGTGG CAGCAGCGCG GGGGGCCTGC AGCACCTCGT GTCCATCCGC 
ACGGCCCCAG ACCTCCTGCC ACACAAGCCG ATGCCCGCCT CCGCCTTCAT AGAGGATGGT 
CGCTTCACCC TCTCCATGCC CCACGTGCAG GATGCCTCAC TCATCAGGTG GTTCTACATC 
GTGGTGGTAC CCATCGACCG CGTGGGTGGG AGTGTGCTGG CCCCGAGATG GAGCACGCCT 
GAGGAGCTGG AGCTGGACGA GCTTCTGGAG GCCATCGAGC AGGGCGGTGG GGAGCGGCGA 
CGACGGCGGC AGGCGGAGCG GCTGAAGCCA TATGTGGCTG CACAGGTGGA CGTGCTCCCC 
GAGAGCTTCA CCCTAGGAGA CAAGAAGAGC TACCGGGGCT TCTACAACCG GCCTCTGTCT 
CCGGACCTGA GCTATCAGTG CTTTGTGCTC GCCTCCCTGA AGGAACCCGT GGACCAGAAG 
CGCTATGCCT CCAGCCCCTA CTCTGATGAG ATCGTGGTCC AAGTGACACC AGCCCAGCAG 
CAGGAGGAGC CTGAGATGCT GTGGGTGACA GGCCCCGTCC TCGCAGTCAT CCTCATCATC 
CTCATTGTCA TCGCCATCCT CCTGTTCAAG AGGAAGAGGA CTCACTCCCC ATCATCCAAG 
GACGAGCAGT CAATCGGGCT GAAGGACTCC TTGCTGGCCC ATTCCTCTGA CCCTGTGGAG 
ATGCGGAGGC TCAACTACCA GACTCCAGGT TCCAGTGTCC CCAGTTGCCC GAATATCTCA 
AGTATGCGAG ACCACCCGCC CATCCCCATC ACTGACCTGG CCGACAACAT TGAGCGCCTC 
AAAGCCAACG ACGGCCTCAA GTTCTCCCAG GAGTACGAGT CCATTGACCC TGGACAGCAG 
TTCACCTGGG AGAATTCAAA CCTGGAAGTG AACAAGCCCA AGAACCGCTA TGCAAACGTC 
ATCGCCTACG ACCACTCCCG AGTCATCCTT ACCTCCGTCG ATGGTGTCCC AGGGAGTGAC 
TATATCAACG CCAACTACAT CGATGGCTAC CGCAAGCAGA ACGCCTACAT TGCCACGCAG 
GGCCCACTGC CCGAGACCAT GGGCGATTTC TGGCGGATGG TGTGGGAACA GCGCACAGCC 
ACCGTGGTCA TGATGACACG GCTGGAGGAG AAGTCTCGGG TGAAGTGTGA TCAGTACTGG 
CCAGCCCGGG GTACCGAGAC CCACGGCCTC ATTCAGGTGA CCCTGCTGGA CACGGTGGAG 
CTGGCCACAT ACACTGTGCG CACCTTTGCA CTCCACAAGA GTGGCTCCAG CGAGAAGCGG 
GAGCTGCGTC AGTTCCAGTT CATGGCCTGG CCGGACCACG GGGTTCCCGA GTACCCCACC 
CCCATCCTGG CCTTCCTGCG GCGGGTCAAG GCCTGTAACC CGCTAGACGC AGGGCCCATG 
GTGGTGCACT GCAGCGCGGG CGTGGGCCGC ACGGGCTGTT TCATCGTCAT CGACGCCATG 
CTGGAGCGGA TGAAGCACGA GAAGACAGTG GACATCTATG GCCACGTGAC ATGCATGCGA 
TCGCAGCGGA ACTACATGGT GCAGACAGAG GACCAGTACG TGTTCATCCA CGAGGCGCTG 
CTAGAGGCCG CCACGTGCGG ACACACGGAG GTGCCTGCCC GCAGCCTGTA TGCCCATATC 
CAGAAGCTGG GCCAAGTGCC TCCCGGGGAG AGTGTGACTG CCATGGAGCT CGAGTTCAAG 
CTGCTGGCCA GCTCCAAGGC CCACACATCC CGCTTCATTA GCGCCAACCT GCCCTGCAAC 
AAGTTCAAGA ACCGCCTGGT GAACATCATG CCCTATGAGT TGACCCGTGT GTGTCTGCAG 
CCCATCCGTG GTGTGGAGGG CTCTGACTAC ATCAATGCCA GCTTCTTGGA CGGCTATAGG 
CAGCAGAAGG CCTACATCGC CACGCAGGGG CCCCTGGCAG AGAGCACCGA GGACTTCTGG 
CGCATGCTCT GGGAGCACGG CTCCACCATC GTCGTCATGC TGACCAGGCT TCGGGAGATG 
GGCAGGGAGA AATGCCACCA GTACTGGCCA GCAGAGCGCT CCGCTCGCTA CCAATACTTT 
GTCGTTGACC CGATGGCTGA GTACAACATG CCGCAGTACA TCCTGCGTGA GTTCAAGGTC 
ACAGATGCCC GGGATGGGCA GTCGAGGACA ATCCGGCAGT TCCAGTTCAC AGACTGGCCC 
GAGCAGGGCG TGCCTAAGAC GGGTGAGGGC TTCATCGACT TCATCGGGCA GGTGCACAAG 
ACCAAGGAGC AGTTCGGGCA GGACGGGCCC ATCACGGTGC ACTGCAGTGC CGGCGTGGGC 
CGCACCGGGG TGTTCATCAC TCTGAGCATC GTCCTGGAGC GGATGCGCTA CGAGGGCGTG 
GTCGACATGT TCCAGACCGT GAAGACCCTG CGCACACAGC GCCCAGCCAT GGTGCAGACG 
GAGGACCAGT ACCAGCTGTG CTATCGTGCG GCCCTGGAGT ACCTTGGCAG CTTTGACCAC 
TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_005629110.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629167.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X1, mRNA.

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CloneID	OCe17419
Clone ID Related Accession (Same CDS sequence)	XM_005629110.3 , XM_005629110.2
Accession Version	XM_005629110.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629110.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GTGGCGGCCA 
GAGGAGTCCG AGGACTATGA AACCATAATC AGCGGCCTGA CCCCAGAGAC CACCTATTCC 
ATTACCGTCG CCGCCTACAC CACCAAAGGG GATGGTGCCC GCAGCAAGCC CAAAGTTGTC 
ACAACGACCG GGGCAGTCCC AGGCCGGCCC ACCATGATGG TCAGCACCAC GGCCATGAAC 
ACCGCACTGC TGCAGTGGCA CCCCCCCAAG GAGCTGCCAG GGGAGCTGCT GGGCTACCGG 
CTACAGTACC ACCGTGCCGA TGAGGCAAAG CCCAACATCC TAGATTTTGG CAAGGACGAC 
CAGCACTTTA CAGTCACCGG CCTACACAAG GGAGCCACCT ACATTTTCCG GCTTGCCGCC 
AAGAACCGGG CTGGCCCGGG AGAAGAGTTC GAGAAGGAGA TCACGACTCC CGAGGATGCG 
CCCAGCGGCT TCCCCCAAAA CCTGCGCGTG ATAGGGCTGA CCACCTCTAC CACGGAGCTG 
ACCTGGGACC CCCCGGTGCT GGCGGAGAGG AACGGGCGCA TCACCAACTA CTCCGTGGTG 
TACCGTGACA TCAACAGCCA GCAGGAGCTA CAGAACGTCA CCGCCGACAC CCACCTCATG 
CTCTCCGGCC TCAAGCCGGA CACCACTTAC GACATCAAGG TCCGCGCACG TACCAGCAAG 
GGCGCCGGCC CACTCAGCCC CAGCATCCAG TCCCGGACCA TGCCCATGGA GCAAGTGTTT 
GCCAAGAACT TCCGTGTGGC GGCCGCTATG AAGACGTCTG TGCTGCTCAG CTGGGAGGTC 
CCAGACTCCT ACAAGTCGGC TGTACCCTTC AAGATCCTGT ACAACGGGCA GAGTGTGGAG 
GTGGACGGGC ACTCCATGCG GAAACTGATC GCAGACCTGC AGCCGAACAC GGAGTACTCG 
TTCGTGCTGA TGAACCGTGG CAGCAGCGCG GGGGGCCTGC AGCACCTCGT GTCCATCCGC 
ACGGCCCCAG ACCTCCTGCC ACACAAGCCG ATGCCCGCCT CCGCCTTCAT AGAGGATGGT 
CGCTTCACCC TCTCCATGCC CCACGTGCAG GATGCCTCAC TCATCAGGTG GTTCTACATC 
GTGGTGGTAC CCATCGACCG CGTGGGTGGG AGTGTGCTGG CCCCGAGATG GAGCACGCCT 
GAGGAGCTGG AGCTGGACGA GCTTCTGGAG GCCATCGAGC AGGGCGGTGG GGAGCGGCGA 
CGACGGCGGC AGGCGGAGCG GCTGAAGCCA TATGTGGCTG CACAGGTGGA CGTGCTCCCC 
GAGAGCTTCA CCCTAGGAGA CAAGAAGAGC TACCGGGGCT TCTACAACCG GCCTCTGTCT 
CCGGACCTGA GCTATCAGTG CTTTGTGCTC GCCTCCCTGA AGGAACCCGT GGACCAGAAG 
CGCTATGCCT CCAGCCCCTA CTCTGATGAG ATCGTGGTCC AAGTGACACC AGCCCAGCAG 
CAGGAGGAGC CTGAGATGCT GTGGGTGACA GGCCCCGTCC TCGCAGTCAT CCTCATCATC 
CTCATTGTCA TCGCCATCCT CCTGTTCAAG AGGAAGAGGA CTCACTCCCC ATCATCCAAG 
GACGAGCAGT CAATCGGGCT GAAGGACTCC TTGCTGGCCC ATTCCTCTGA CCCTGTGGAG 
ATGCGGAGGC TCAACTACCA GACTCCAGGT TCCAGTGTCC CCAGTTGCCC GAATATCTCA 
AGTATGCGAG ACCACCCGCC CATCCCCATC ACTGACCTGG CCGACAACAT TGAGCGCCTC 
AAAGCCAACG ACGGCCTCAA GTTCTCCCAG GAGTACGAGT CCATTGACCC TGGACAGCAG 
TTCACCTGGG AGAATTCAAA CCTGGAAGTG AACAAGCCCA AGAACCGCTA TGCAAACGTC 
ATCGCCTACG ACCACTCCCG AGTCATCCTT ACCTCCGTCG ATGGTGTCCC AGGGAGTGAC 
TATATCAACG CCAACTACAT CGATGGCTAC CGCAAGCAGA ACGCCTACAT TGCCACGCAG 
GGCCCACTGC CCGAGACCAT GGGCGATTTC TGGCGGATGG TGTGGGAACA GCGCACAGCC 
ACCGTGGTCA TGATGACACG GCTGGAGGAG AAGTCTCGGG TGAAGTGTGA TCAGTACTGG 
CCAGCCCGGG GTACCGAGAC CCACGGCCTC ATTCAGGTGA CCCTGCTGGA CACGGTGGAG 
CTGGCCACAT ACACTGTGCG CACCTTTGCA CTCCACAAGA GTGGCTCCAG CGAGAAGCGG 
GAGCTGCGTC AGTTCCAGTT CATGGCCTGG CCGGACCACG GGGTTCCCGA GTACCCCACC 
CCCATCCTGG CCTTCCTGCG GCGGGTCAAG GCCTGTAACC CGCTAGACGC AGGGCCCATG 
GTGGTGCACT GCAGCGCGGG CGTGGGCCGC ACGGGCTGTT TCATCGTCAT CGACGCCATG 
CTGGAGCGGA TGAAGCACGA GAAGACAGTG GACATCTATG GCCACGTGAC ATGCATGCGA 
TCGCAGCGGA ACTACATGGT GCAGACAGAG GACCAGTACG TGTTCATCCA CGAGGCGCTG 
CTAGAGGCCG CCACGTGCGG ACACACGGAG GTGCCTGCCC GCAGCCTGTA TGCCCATATC 
CAGAAGCTGG GCCAAGTGCC TCCCGGGGAG AGTGTGACTG CCATGGAGCT CGAGTTCAAG 
CTGCTGGCCA GCTCCAAGGC CCACACATCC CGCTTCATTA GCGCCAACCT GCCCTGCAAC 
AAGTTCAAGA ACCGCCTGGT GAACATCATG CCCTATGAGT TGACCCGTGT GTGTCTGCAG 
CCCATCCGTG GTGTGGAGGG CTCTGACTAC ATCAATGCCA GCTTCTTGGA CGGCTATAGG 
CAGCAGAAGG CCTACATCGC CACGCAGGGG CCCCTGGCAG AGAGCACCGA GGACTTCTGG 
CGCATGCTCT GGGAGCACGG CTCCACCATC GTCGTCATGC TGACCAGGCT TCGGGAGATG 
GGCAGGGAGA AATGCCACCA GTACTGGCCA GCAGAGCGCT CCGCTCGCTA CCAATACTTT 
GTCGTTGACC CGATGGCTGA GTACAACATG CCGCAGTACA TCCTGCGTGA GTTCAAGGTC 
ACAGATGCCC GGGATGGGCA GTCGAGGACA ATCCGGCAGT TCCAGTTCAC AGACTGGCCC 
GAGCAGGGCG TGCCTAAGAC GGGTGAGGGC TTCATCGACT TCATCGGGCA GGTGCACAAG 
ACCAAGGAGC AGTTCGGGCA GGACGGGCCC ATCACGGTGC ACTGCAGTGC CGGCGTGGGC 
CGCACCGGGG TGTTCATCAC TCTGAGCATC GTCCTGGAGC GGATGCGCTA CGAGGGCGTG 
GTCGACATGT TCCAGACCGT GAAGACCCTG CGCACACAGC GCCCAGCCAT GGTGCAGACG 
GAGGACCAGT ACCAGCTGTG CTATCGTGCG GCCCTGGAGT ACCTTGGCAG CTTTGACCAC 
TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629167.1
CDS	125..5896
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGGSPIRGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPAASRNGVITQYSVAYEAVDGEDRGRHVVESISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQWRPEESEDYETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGSSVPSCPNISSMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_005629110.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629110.2

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GTGGCGGCCA 
GAGGAGTCCG AGGACTATGA AACCATAATC AGCGGCCTGA CCCCAGAGAC CACCTATTCC 
ATTACCGTCG CCGCCTACAC CACCAAAGGG GATGGTGCCC GCAGCAAGCC CAAAGTTGTC 
ACAACGACCG GGGCAGTCCC AGGCCGGCCC ACCATGATGG TCAGCACCAC GGCCATGAAC 
ACCGCACTGC TGCAGTGGCA CCCCCCCAAG GAGCTGCCAG GGGAGCTGCT GGGCTACCGG 
CTACAGTACC ACCGTGCCGA TGAGGCAAAG CCCAACATCC TAGATTTTGG CAAGGACGAC 
CAGCACTTTA CAGTCACCGG CCTACACAAG GGAGCCACCT ACATTTTCCG GCTTGCCGCC 
AAGAACCGGG CTGGCCCGGG AGAAGAGTTC GAGAAGGAGA TCACGACTCC CGAGGATGCG 
CCCAGCGGCT TCCCCCAAAA CCTGCGCGTG ATAGGGCTGA CCACCTCTAC CACGGAGCTG 
ACCTGGGACC CCCCGGTGCT GGCGGAGAGG AACGGGCGCA TCACCAACTA CTCCGTGGTG 
TACCGTGACA TCAACAGCCA GCAGGAGCTA CAGAACGTCA CCGCCGACAC CCACCTCATG 
CTCTCCGGCC TCAAGCCGGA CACCACTTAC GACATCAAGG TCCGCGCACG TACCAGCAAG 
GGCGCCGGCC CACTCAGCCC CAGCATCCAG TCCCGGACCA TGCCCATGGA GCAAGTGTTT 
GCCAAGAACT TCCGTGTGGC GGCCGCTATG AAGACGTCTG TGCTGCTCAG CTGGGAGGTC 
CCAGACTCCT ACAAGTCGGC TGTACCCTTC AAGATCCTGT ACAACGGGCA GAGTGTGGAG 
GTGGACGGGC ACTCCATGCG GAAACTGATC GCAGACCTGC AGCCGAACAC GGAGTACTCG 
TTCGTGCTGA TGAACCGTGG CAGCAGCGCG GGGGGCCTGC AGCACCTCGT GTCCATCCGC 
ACGGCCCCAG ACCTCCTGCC ACACAAGCCG ATGCCCGCCT CCGCCTTCAT AGAGGATGGT 
CGCTTCACCC TCTCCATGCC CCACGTGCAG GATGCCTCAC TCATCAGGTG GTTCTACATC 
GTGGTGGTAC CCATCGACCG CGTGGGTGGG AGTGTGCTGG CCCCGAGATG GAGCACGCCT 
GAGGAGCTGG AGCTGGACGA GCTTCTGGAG GCCATCGAGC AGGGCGGTGG GGAGCGGCGA 
CGACGGCGGC AGGCGGAGCG GCTGAAGCCA TATGTGGCTG CACAGGTGGA CGTGCTCCCC 
GAGAGCTTCA CCCTAGGAGA CAAGAAGAGC TACCGGGGCT TCTACAACCG GCCTCTGTCT 
CCGGACCTGA GCTATCAGTG CTTTGTGCTC GCCTCCCTGA AGGAACCCGT GGACCAGAAG 
CGCTATGCCT CCAGCCCCTA CTCTGATGAG ATCGTGGTCC AAGTGACACC AGCCCAGCAG 
CAGGAGGAGC CTGAGATGCT GTGGGTGACA GGCCCCGTCC TCGCAGTCAT CCTCATCATC 
CTCATTGTCA TCGCCATCCT CCTGTTCAAG AGGAAGAGGA CTCACTCCCC ATCATCCAAG 
GACGAGCAGT CAATCGGGCT GAAGGACTCC TTGCTGGCCC ATTCCTCTGA CCCTGTGGAG 
ATGCGGAGGC TCAACTACCA GACTCCAGGT TCCAGTGTCC CCAGTTGCCC GAATATCTCA 
AGTATGCGAG ACCACCCGCC CATCCCCATC ACTGACCTGG CCGACAACAT TGAGCGCCTC 
AAAGCCAACG ACGGCCTCAA GTTCTCCCAG GAGTACGAGT CCATTGACCC TGGACAGCAG 
TTCACCTGGG AGAATTCAAA CCTGGAAGTG AACAAGCCCA AGAACCGCTA TGCAAACGTC 
ATCGCCTACG ACCACTCCCG AGTCATCCTT ACCTCCGTCG ATGGTGTCCC AGGGAGTGAC 
TATATCAACG CCAACTACAT CGATGGCTAC CGCAAGCAGA ACGCCTACAT TGCCACGCAG 
GGCCCACTGC CCGAGACCAT GGGCGATTTC TGGCGGATGG TGTGGGAACA GCGCACAGCC 
ACCGTGGTCA TGATGACACG GCTGGAGGAG AAGTCTCGGG TGAAGTGTGA TCAGTACTGG 
CCAGCCCGGG GTACCGAGAC CCACGGCCTC ATTCAGGTGA CCCTGCTGGA CACGGTGGAG 
CTGGCCACAT ACACTGTGCG CACCTTTGCA CTCCACAAGA GTGGCTCCAG CGAGAAGCGG 
GAGCTGCGTC AGTTCCAGTT CATGGCCTGG CCGGACCACG GGGTTCCCGA GTACCCCACC 
CCCATCCTGG CCTTCCTGCG GCGGGTCAAG GCCTGTAACC CGCTAGACGC AGGGCCCATG 
GTGGTGCACT GCAGCGCGGG CGTGGGCCGC ACGGGCTGTT TCATCGTCAT CGACGCCATG 
CTGGAGCGGA TGAAGCACGA GAAGACAGTG GACATCTATG GCCACGTGAC ATGCATGCGA 
TCGCAGCGGA ACTACATGGT GCAGACAGAG GACCAGTACG TGTTCATCCA CGAGGCGCTG 
CTAGAGGCCG CCACGTGCGG ACACACGGAG GTGCCTGCCC GCAGCCTGTA TGCCCATATC 
CAGAAGCTGG GCCAAGTGCC TCCCGGGGAG AGTGTGACTG CCATGGAGCT CGAGTTCAAG 
CTGCTGGCCA GCTCCAAGGC CCACACATCC CGCTTCATTA GCGCCAACCT GCCCTGCAAC 
AAGTTCAAGA ACCGCCTGGT GAACATCATG CCCTATGAGT TGACCCGTGT GTGTCTGCAG 
CCCATCCGTG GTGTGGAGGG CTCTGACTAC ATCAATGCCA GCTTCTTGGA CGGCTATAGG 
CAGCAGAAGG CCTACATCGC CACGCAGGGG CCCCTGGCAG AGAGCACCGA GGACTTCTGG 
CGCATGCTCT GGGAGCACGG CTCCACCATC GTCGTCATGC TGACCAGGCT TCGGGAGATG 
GGCAGGGAGA AATGCCACCA GTACTGGCCA GCAGAGCGCT CCGCTCGCTA CCAATACTTT 
GTCGTTGACC CGATGGCTGA GTACAACATG CCGCAGTACA TCCTGCGTGA GTTCAAGGTC 
ACAGATGCCC GGGATGGGCA GTCGAGGACA ATCCGGCAGT TCCAGTTCAC AGACTGGCCC 
GAGCAGGGCG TGCCTAAGAC GGGTGAGGGC TTCATCGACT TCATCGGGCA GGTGCACAAG 
ACCAAGGAGC AGTTCGGGCA GGACGGGCCC ATCACGGTGC ACTGCAGTGC CGGCGTGGGC 
CGCACCGGGG TGTTCATCAC TCTGAGCATC GTCCTGGAGC GGATGCGCTA CGAGGGCGTG 
GTCGACATGT TCCAGACCGT GAAGACCCTG CGCACACAGC GCCCAGCCAT GGTGCAGACG 
GAGGACCAGT ACCAGCTGTG CTATCGTGCG GCCCTGGAGT ACCTTGGCAG CTTTGACCAC 
TATGCAACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_005629111.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629168.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X2, mRNA.

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CloneID	OCe17420
Clone ID Related Accession (Same CDS sequence)	XM_005629111.2 , XM_005629111.3
Accession Version	XM_005629111.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629111.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCACCCCCTC AGCCCCTCCC CAGAAGGTGA CCTGTGTGAG CACGGGCTCC 
ACCACGGTTC GGGTAAGTTG GGTCCCGCCG CCAGCTGCCA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCAGTGGAC GGCGAGGACC GAGGGCGGCA TGTGGTGGAG 
AGCATCAGTC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACTGACGTG GGCCCCGGCC CAGAGAGCAG CCCGGTGCTC 
GTGCGCACAG ACGAGGACGT GCCCAGTGGA CCGCCACGGA AGGTGGAGGT GGAACCACTG 
AATTCCACTG CTGTGCGTGT CTCTTGGAAG CTGCCCGTCC CCAGCAAGCA GCACGGCCAG 
ATTCGCGGCT ACCAGGTCAC CTATGTGCGG CTAGAGAATG GCGAGCCCCG TGGCCCACCC 
ATCATCCAGG ACGTCATGCT GGCTGAGGCC CAGTGGCGGC CAGAGGAGTC CGAGGACTAT 
GAAACCATAA TCAGCGGCCT GACCCCAGAG ACCACCTATT CCATTACCGT CGCCGCCTAC 
ACCACCAAAG GGGATGGTGC CCGCAGCAAG CCCAAAGTTG TCACAACGAC CGGGGCAGTC 
CCAGGCCGGC CCACCATGAT GGTCAGCACC ACGGCCATGA ACACCGCACT GCTGCAGTGG 
CACCCCCCCA AGGAGCTGCC AGGGGAGCTG CTGGGCTACC GGCTACAGTA CCACCGTGCC 
GATGAGGCAA AGCCCAACAT CCTAGATTTT GGCAAGGACG ACCAGCACTT TACAGTCACC 
GGCCTACACA AGGGAGCCAC CTACATTTTC CGGCTTGCCG CCAAGAACCG GGCTGGCCCG 
GGAGAAGAGT TCGAGAAGGA GATCACGACT CCCGAGGATG CGCCCAGCGG CTTCCCCCAA 
AACCTGCGCG TGATAGGGCT GACCACCTCT ACCACGGAGC TGACCTGGGA CCCCCCGGTG 
CTGGCGGAGA GGAACGGGCG CATCACCAAC TACTCCGTGG TGTACCGTGA CATCAACAGC 
CAGCAGGAGC TACAGAACGT CACCGCCGAC ACCCACCTCA TGCTCTCCGG CCTCAAGCCG 
GACACCACTT ACGACATCAA GGTCCGCGCA CGTACCAGCA AGGGCGCCGG CCCACTCAGC 
CCCAGCATCC AGTCCCGGAC CATGCCCATG GAGCAAGTGT TTGCCAAGAA CTTCCGTGTG 
GCGGCCGCTA TGAAGACGTC TGTGCTGCTC AGCTGGGAGG TCCCAGACTC CTACAAGTCG 
GCTGTACCCT TCAAGATCCT GTACAACGGG CAGAGTGTGG AGGTGGACGG GCACTCCATG 
CGGAAACTGA TCGCAGACCT GCAGCCGAAC ACGGAGTACT CGTTCGTGCT GATGAACCGT 
GGCAGCAGCG CGGGGGGCCT GCAGCACCTC GTGTCCATCC GCACGGCCCC AGACCTCCTG 
CCACACAAGC CGATGCCCGC CTCCGCCTTC ATAGAGGATG GTCGCTTCAC CCTCTCCATG 
CCCCACGTGC AGGATGCCTC ACTCATCAGG TGGTTCTACA TCGTGGTGGT ACCCATCGAC 
CGCGTGGGTG GGAGTGTGCT GGCCCCGAGA TGGAGCACGC CTGAGGAGCT GGAGCTGGAC 
GAGCTTCTGG AGGCCATCGA GCAGGGCGGT GGGGAGCGGC GACGACGGCG GCAGGCGGAG 
CGGCTGAAGC CATATGTGGC TGCACAGGTG GACGTGCTCC CCGAGAGCTT CACCCTAGGA 
GACAAGAAGA GCTACCGGGG CTTCTACAAC CGGCCTCTGT CTCCGGACCT GAGCTATCAG 
TGCTTTGTGC TCGCCTCCCT GAAGGAACCC GTGGACCAGA AGCGCTATGC CTCCAGCCCC 
TACTCTGATG AGATCGTGGT CCAAGTGACA CCAGCCCAGC AGCAGGAGGA GCCTGAGATG 
CTGTGGGTGA CAGGCCCCGT CCTCGCAGTC ATCCTCATCA TCCTCATTGT CATCGCCATC 
CTCCTGTTCA AGAGGAAGAG GACTCACTCC CCATCATCCA AGGACGAGCA GTCAATCGGG 
CTGAAGGACT CCTTGCTGGC CCATTCCTCT GACCCTGTGG AGATGCGGAG GCTCAACTAC 
CAGACTCCAG GTTCCAGTGT CCCCAGTTGC CCGAATATCT CAAGTATGCG AGACCACCCG 
CCCATCCCCA TCACTGACCT GGCCGACAAC ATTGAGCGCC TCAAAGCCAA CGACGGCCTC 
AAGTTCTCCC AGGAGTACGA GTCCATTGAC CCTGGACAGC AGTTCACCTG GGAGAATTCA 
AACCTGGAAG TGAACAAGCC CAAGAACCGC TATGCAAACG TCATCGCCTA CGACCACTCC 
CGAGTCATCC TTACCTCCGT CGATGGTGTC CCAGGGAGTG ACTATATCAA CGCCAACTAC 
ATCGATGGCT ACCGCAAGCA GAACGCCTAC ATTGCCACGC AGGGCCCACT GCCCGAGACC 
ATGGGCGATT TCTGGCGGAT GGTGTGGGAA CAGCGCACAG CCACCGTGGT CATGATGACA 
CGGCTGGAGG AGAAGTCTCG GGTGAAGTGT GATCAGTACT GGCCAGCCCG GGGTACCGAG 
ACCCACGGCC TCATTCAGGT GACCCTGCTG GACACGGTGG AGCTGGCCAC ATACACTGTG 
CGCACCTTTG CACTCCACAA GAGTGGCTCC AGCGAGAAGC GGGAGCTGCG TCAGTTCCAG 
TTCATGGCCT GGCCGGACCA CGGGGTTCCC GAGTACCCCA CCCCCATCCT GGCCTTCCTG 
CGGCGGGTCA AGGCCTGTAA CCCGCTAGAC GCAGGGCCCA TGGTGGTGCA CTGCAGCGCG 
GGCGTGGGCC GCACGGGCTG TTTCATCGTC ATCGACGCCA TGCTGGAGCG GATGAAGCAC 
GAGAAGACAG TGGACATCTA TGGCCACGTG ACATGCATGC GATCGCAGCG GAACTACATG 
GTGCAGACAG AGGACCAGTA CGTGTTCATC CACGAGGCGC TGCTAGAGGC CGCCACGTGC 
GGACACACGG AGGTGCCTGC CCGCAGCCTG TATGCCCATA TCCAGAAGCT GGGCCAAGTG 
CCTCCCGGGG AGAGTGTGAC TGCCATGGAG CTCGAGTTCA AGCTGCTGGC CAGCTCCAAG 
GCCCACACAT CCCGCTTCAT TAGCGCCAAC CTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTGAACATCA TGCCCTATGA GTTGACCCGT GTGTGTCTGC AGCCCATCCG TGGTGTGGAG 
GGCTCTGACT ACATCAATGC CAGCTTCTTG GACGGCTATA GGCAGCAGAA GGCCTACATC 
GCCACGCAGG GGCCCCTGGC AGAGAGCACC GAGGACTTCT GGCGCATGCT CTGGGAGCAC 
GGCTCCACCA TCGTCGTCAT GCTGACCAGG CTTCGGGAGA TGGGCAGGGA GAAATGCCAC 
CAGTACTGGC CAGCAGAGCG CTCCGCTCGC TACCAATACT TTGTCGTTGA CCCGATGGCT 
GAGTACAACA TGCCGCAGTA CATCCTGCGT GAGTTCAAGG TCACAGATGC CCGGGATGGG 
CAGTCGAGGA CAATCCGGCA GTTCCAGTTC ACAGACTGGC CCGAGCAGGG CGTGCCTAAG 
ACGGGTGAGG GCTTCATCGA CTTCATCGGG CAGGTGCACA AGACCAAGGA GCAGTTCGGG 
CAGGACGGGC CCATCACGGT GCACTGCAGT GCCGGCGTGG GCCGCACCGG GGTGTTCATC 
ACTCTGAGCA TCGTCCTGGA GCGGATGCGC TACGAGGGCG TGGTCGACAT GTTCCAGACC 
GTGAAGACCC TGCGCACACA GCGCCCAGCC ATGGTGCAGA CGGAGGACCA GTACCAGCTG 
TGCTATCGTG CGGCCCTGGA GTACCTTGGC AGCTTTGACC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629168.1
CDS	125..5878
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPAASRNGVITQYSVAYEAVDGEDRGRHVVESISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQWRPEESEDYETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGSSVPSCPNISSMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_005629111.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629111.2

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCACCCCCTC AGCCCCTCCC CAGAAGGTGA CCTGTGTGAG CACGGGCTCC 
ACCACGGTTC GGGTAAGTTG GGTCCCGCCG CCAGCTGCCA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCAGTGGAC GGCGAGGACC GAGGGCGGCA TGTGGTGGAG 
AGCATCAGTC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACTGACGTG GGCCCCGGCC CAGAGAGCAG CCCGGTGCTC 
GTGCGCACAG ACGAGGACGT GCCCAGTGGA CCGCCACGGA AGGTGGAGGT GGAACCACTG 
AATTCCACTG CTGTGCGTGT CTCTTGGAAG CTGCCCGTCC CCAGCAAGCA GCACGGCCAG 
ATTCGCGGCT ACCAGGTCAC CTATGTGCGG CTAGAGAATG GCGAGCCCCG TGGCCCACCC 
ATCATCCAGG ACGTCATGCT GGCTGAGGCC CAGTGGCGGC CAGAGGAGTC CGAGGACTAT 
GAAACCATAA TCAGCGGCCT GACCCCAGAG ACCACCTATT CCATTACCGT CGCCGCCTAC 
ACCACCAAAG GGGATGGTGC CCGCAGCAAG CCCAAAGTTG TCACAACGAC CGGGGCAGTC 
CCAGGCCGGC CCACCATGAT GGTCAGCACC ACGGCCATGA ACACCGCACT GCTGCAGTGG 
CACCCCCCCA AGGAGCTGCC AGGGGAGCTG CTGGGCTACC GGCTACAGTA CCACCGTGCC 
GATGAGGCAA AGCCCAACAT CCTAGATTTT GGCAAGGACG ACCAGCACTT TACAGTCACC 
GGCCTACACA AGGGAGCCAC CTACATTTTC CGGCTTGCCG CCAAGAACCG GGCTGGCCCG 
GGAGAAGAGT TCGAGAAGGA GATCACGACT CCCGAGGATG CGCCCAGCGG CTTCCCCCAA 
AACCTGCGCG TGATAGGGCT GACCACCTCT ACCACGGAGC TGACCTGGGA CCCCCCGGTG 
CTGGCGGAGA GGAACGGGCG CATCACCAAC TACTCCGTGG TGTACCGTGA CATCAACAGC 
CAGCAGGAGC TACAGAACGT CACCGCCGAC ACCCACCTCA TGCTCTCCGG CCTCAAGCCG 
GACACCACTT ACGACATCAA GGTCCGCGCA CGTACCAGCA AGGGCGCCGG CCCACTCAGC 
CCCAGCATCC AGTCCCGGAC CATGCCCATG GAGCAAGTGT TTGCCAAGAA CTTCCGTGTG 
GCGGCCGCTA TGAAGACGTC TGTGCTGCTC AGCTGGGAGG TCCCAGACTC CTACAAGTCG 
GCTGTACCCT TCAAGATCCT GTACAACGGG CAGAGTGTGG AGGTGGACGG GCACTCCATG 
CGGAAACTGA TCGCAGACCT GCAGCCGAAC ACGGAGTACT CGTTCGTGCT GATGAACCGT 
GGCAGCAGCG CGGGGGGCCT GCAGCACCTC GTGTCCATCC GCACGGCCCC AGACCTCCTG 
CCACACAAGC CGATGCCCGC CTCCGCCTTC ATAGAGGATG GTCGCTTCAC CCTCTCCATG 
CCCCACGTGC AGGATGCCTC ACTCATCAGG TGGTTCTACA TCGTGGTGGT ACCCATCGAC 
CGCGTGGGTG GGAGTGTGCT GGCCCCGAGA TGGAGCACGC CTGAGGAGCT GGAGCTGGAC 
GAGCTTCTGG AGGCCATCGA GCAGGGCGGT GGGGAGCGGC GACGACGGCG GCAGGCGGAG 
CGGCTGAAGC CATATGTGGC TGCACAGGTG GACGTGCTCC CCGAGAGCTT CACCCTAGGA 
GACAAGAAGA GCTACCGGGG CTTCTACAAC CGGCCTCTGT CTCCGGACCT GAGCTATCAG 
TGCTTTGTGC TCGCCTCCCT GAAGGAACCC GTGGACCAGA AGCGCTATGC CTCCAGCCCC 
TACTCTGATG AGATCGTGGT CCAAGTGACA CCAGCCCAGC AGCAGGAGGA GCCTGAGATG 
CTGTGGGTGA CAGGCCCCGT CCTCGCAGTC ATCCTCATCA TCCTCATTGT CATCGCCATC 
CTCCTGTTCA AGAGGAAGAG GACTCACTCC CCATCATCCA AGGACGAGCA GTCAATCGGG 
CTGAAGGACT CCTTGCTGGC CCATTCCTCT GACCCTGTGG AGATGCGGAG GCTCAACTAC 
CAGACTCCAG GTTCCAGTGT CCCCAGTTGC CCGAATATCT CAAGTATGCG AGACCACCCG 
CCCATCCCCA TCACTGACCT GGCCGACAAC ATTGAGCGCC TCAAAGCCAA CGACGGCCTC 
AAGTTCTCCC AGGAGTACGA GTCCATTGAC CCTGGACAGC AGTTCACCTG GGAGAATTCA 
AACCTGGAAG TGAACAAGCC CAAGAACCGC TATGCAAACG TCATCGCCTA CGACCACTCC 
CGAGTCATCC TTACCTCCGT CGATGGTGTC CCAGGGAGTG ACTATATCAA CGCCAACTAC 
ATCGATGGCT ACCGCAAGCA GAACGCCTAC ATTGCCACGC AGGGCCCACT GCCCGAGACC 
ATGGGCGATT TCTGGCGGAT GGTGTGGGAA CAGCGCACAG CCACCGTGGT CATGATGACA 
CGGCTGGAGG AGAAGTCTCG GGTGAAGTGT GATCAGTACT GGCCAGCCCG GGGTACCGAG 
ACCCACGGCC TCATTCAGGT GACCCTGCTG GACACGGTGG AGCTGGCCAC ATACACTGTG 
CGCACCTTTG CACTCCACAA GAGTGGCTCC AGCGAGAAGC GGGAGCTGCG TCAGTTCCAG 
TTCATGGCCT GGCCGGACCA CGGGGTTCCC GAGTACCCCA CCCCCATCCT GGCCTTCCTG 
CGGCGGGTCA AGGCCTGTAA CCCGCTAGAC GCAGGGCCCA TGGTGGTGCA CTGCAGCGCG 
GGCGTGGGCC GCACGGGCTG TTTCATCGTC ATCGACGCCA TGCTGGAGCG GATGAAGCAC 
GAGAAGACAG TGGACATCTA TGGCCACGTG ACATGCATGC GATCGCAGCG GAACTACATG 
GTGCAGACAG AGGACCAGTA CGTGTTCATC CACGAGGCGC TGCTAGAGGC CGCCACGTGC 
GGACACACGG AGGTGCCTGC CCGCAGCCTG TATGCCCATA TCCAGAAGCT GGGCCAAGTG 
CCTCCCGGGG AGAGTGTGAC TGCCATGGAG CTCGAGTTCA AGCTGCTGGC CAGCTCCAAG 
GCCCACACAT CCCGCTTCAT TAGCGCCAAC CTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTGAACATCA TGCCCTATGA GTTGACCCGT GTGTGTCTGC AGCCCATCCG TGGTGTGGAG 
GGCTCTGACT ACATCAATGC CAGCTTCTTG GACGGCTATA GGCAGCAGAA GGCCTACATC 
GCCACGCAGG GGCCCCTGGC AGAGAGCACC GAGGACTTCT GGCGCATGCT CTGGGAGCAC 
GGCTCCACCA TCGTCGTCAT GCTGACCAGG CTTCGGGAGA TGGGCAGGGA GAAATGCCAC 
CAGTACTGGC CAGCAGAGCG CTCCGCTCGC TACCAATACT TTGTCGTTGA CCCGATGGCT 
GAGTACAACA TGCCGCAGTA CATCCTGCGT GAGTTCAAGG TCACAGATGC CCGGGATGGG 
CAGTCGAGGA CAATCCGGCA GTTCCAGTTC ACAGACTGGC CCGAGCAGGG CGTGCCTAAG 
ACGGGTGAGG GCTTCATCGA CTTCATCGGG CAGGTGCACA AGACCAAGGA GCAGTTCGGG 
CAGGACGGGC CCATCACGGT GCACTGCAGT GCCGGCGTGG GCCGCACCGG GGTGTTCATC 
ACTCTGAGCA TCGTCCTGGA GCGGATGCGC TACGAGGGCG TGGTCGACAT GTTCCAGACC 
GTGAAGACCC TGCGCACACA GCGCCCAGCC ATGGTGCAGA CGGAGGACCA GTACCAGCTG 
TGCTATCGTG CGGCCCTGGA GTACCTTGGC AGCTTTGACC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_005629111.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629168.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X2, mRNA.

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CloneID	OCe17420
Clone ID Related Accession (Same CDS sequence)	XM_005629111.2 , XM_005629111.3
Accession Version	XM_005629111.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629111.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCACCCCCTC AGCCCCTCCC CAGAAGGTGA CCTGTGTGAG CACGGGCTCC 
ACCACGGTTC GGGTAAGTTG GGTCCCGCCG CCAGCTGCCA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCAGTGGAC GGCGAGGACC GAGGGCGGCA TGTGGTGGAG 
AGCATCAGTC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACTGACGTG GGCCCCGGCC CAGAGAGCAG CCCGGTGCTC 
GTGCGCACAG ACGAGGACGT GCCCAGTGGA CCGCCACGGA AGGTGGAGGT GGAACCACTG 
AATTCCACTG CTGTGCGTGT CTCTTGGAAG CTGCCCGTCC CCAGCAAGCA GCACGGCCAG 
ATTCGCGGCT ACCAGGTCAC CTATGTGCGG CTAGAGAATG GCGAGCCCCG TGGCCCACCC 
ATCATCCAGG ACGTCATGCT GGCTGAGGCC CAGTGGCGGC CAGAGGAGTC CGAGGACTAT 
GAAACCATAA TCAGCGGCCT GACCCCAGAG ACCACCTATT CCATTACCGT CGCCGCCTAC 
ACCACCAAAG GGGATGGTGC CCGCAGCAAG CCCAAAGTTG TCACAACGAC CGGGGCAGTC 
CCAGGCCGGC CCACCATGAT GGTCAGCACC ACGGCCATGA ACACCGCACT GCTGCAGTGG 
CACCCCCCCA AGGAGCTGCC AGGGGAGCTG CTGGGCTACC GGCTACAGTA CCACCGTGCC 
GATGAGGCAA AGCCCAACAT CCTAGATTTT GGCAAGGACG ACCAGCACTT TACAGTCACC 
GGCCTACACA AGGGAGCCAC CTACATTTTC CGGCTTGCCG CCAAGAACCG GGCTGGCCCG 
GGAGAAGAGT TCGAGAAGGA GATCACGACT CCCGAGGATG CGCCCAGCGG CTTCCCCCAA 
AACCTGCGCG TGATAGGGCT GACCACCTCT ACCACGGAGC TGACCTGGGA CCCCCCGGTG 
CTGGCGGAGA GGAACGGGCG CATCACCAAC TACTCCGTGG TGTACCGTGA CATCAACAGC 
CAGCAGGAGC TACAGAACGT CACCGCCGAC ACCCACCTCA TGCTCTCCGG CCTCAAGCCG 
GACACCACTT ACGACATCAA GGTCCGCGCA CGTACCAGCA AGGGCGCCGG CCCACTCAGC 
CCCAGCATCC AGTCCCGGAC CATGCCCATG GAGCAAGTGT TTGCCAAGAA CTTCCGTGTG 
GCGGCCGCTA TGAAGACGTC TGTGCTGCTC AGCTGGGAGG TCCCAGACTC CTACAAGTCG 
GCTGTACCCT TCAAGATCCT GTACAACGGG CAGAGTGTGG AGGTGGACGG GCACTCCATG 
CGGAAACTGA TCGCAGACCT GCAGCCGAAC ACGGAGTACT CGTTCGTGCT GATGAACCGT 
GGCAGCAGCG CGGGGGGCCT GCAGCACCTC GTGTCCATCC GCACGGCCCC AGACCTCCTG 
CCACACAAGC CGATGCCCGC CTCCGCCTTC ATAGAGGATG GTCGCTTCAC CCTCTCCATG 
CCCCACGTGC AGGATGCCTC ACTCATCAGG TGGTTCTACA TCGTGGTGGT ACCCATCGAC 
CGCGTGGGTG GGAGTGTGCT GGCCCCGAGA TGGAGCACGC CTGAGGAGCT GGAGCTGGAC 
GAGCTTCTGG AGGCCATCGA GCAGGGCGGT GGGGAGCGGC GACGACGGCG GCAGGCGGAG 
CGGCTGAAGC CATATGTGGC TGCACAGGTG GACGTGCTCC CCGAGAGCTT CACCCTAGGA 
GACAAGAAGA GCTACCGGGG CTTCTACAAC CGGCCTCTGT CTCCGGACCT GAGCTATCAG 
TGCTTTGTGC TCGCCTCCCT GAAGGAACCC GTGGACCAGA AGCGCTATGC CTCCAGCCCC 
TACTCTGATG AGATCGTGGT CCAAGTGACA CCAGCCCAGC AGCAGGAGGA GCCTGAGATG 
CTGTGGGTGA CAGGCCCCGT CCTCGCAGTC ATCCTCATCA TCCTCATTGT CATCGCCATC 
CTCCTGTTCA AGAGGAAGAG GACTCACTCC CCATCATCCA AGGACGAGCA GTCAATCGGG 
CTGAAGGACT CCTTGCTGGC CCATTCCTCT GACCCTGTGG AGATGCGGAG GCTCAACTAC 
CAGACTCCAG GTTCCAGTGT CCCCAGTTGC CCGAATATCT CAAGTATGCG AGACCACCCG 
CCCATCCCCA TCACTGACCT GGCCGACAAC ATTGAGCGCC TCAAAGCCAA CGACGGCCTC 
AAGTTCTCCC AGGAGTACGA GTCCATTGAC CCTGGACAGC AGTTCACCTG GGAGAATTCA 
AACCTGGAAG TGAACAAGCC CAAGAACCGC TATGCAAACG TCATCGCCTA CGACCACTCC 
CGAGTCATCC TTACCTCCGT CGATGGTGTC CCAGGGAGTG ACTATATCAA CGCCAACTAC 
ATCGATGGCT ACCGCAAGCA GAACGCCTAC ATTGCCACGC AGGGCCCACT GCCCGAGACC 
ATGGGCGATT TCTGGCGGAT GGTGTGGGAA CAGCGCACAG CCACCGTGGT CATGATGACA 
CGGCTGGAGG AGAAGTCTCG GGTGAAGTGT GATCAGTACT GGCCAGCCCG GGGTACCGAG 
ACCCACGGCC TCATTCAGGT GACCCTGCTG GACACGGTGG AGCTGGCCAC ATACACTGTG 
CGCACCTTTG CACTCCACAA GAGTGGCTCC AGCGAGAAGC GGGAGCTGCG TCAGTTCCAG 
TTCATGGCCT GGCCGGACCA CGGGGTTCCC GAGTACCCCA CCCCCATCCT GGCCTTCCTG 
CGGCGGGTCA AGGCCTGTAA CCCGCTAGAC GCAGGGCCCA TGGTGGTGCA CTGCAGCGCG 
GGCGTGGGCC GCACGGGCTG TTTCATCGTC ATCGACGCCA TGCTGGAGCG GATGAAGCAC 
GAGAAGACAG TGGACATCTA TGGCCACGTG ACATGCATGC GATCGCAGCG GAACTACATG 
GTGCAGACAG AGGACCAGTA CGTGTTCATC CACGAGGCGC TGCTAGAGGC CGCCACGTGC 
GGACACACGG AGGTGCCTGC CCGCAGCCTG TATGCCCATA TCCAGAAGCT GGGCCAAGTG 
CCTCCCGGGG AGAGTGTGAC TGCCATGGAG CTCGAGTTCA AGCTGCTGGC CAGCTCCAAG 
GCCCACACAT CCCGCTTCAT TAGCGCCAAC CTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTGAACATCA TGCCCTATGA GTTGACCCGT GTGTGTCTGC AGCCCATCCG TGGTGTGGAG 
GGCTCTGACT ACATCAATGC CAGCTTCTTG GACGGCTATA GGCAGCAGAA GGCCTACATC 
GCCACGCAGG GGCCCCTGGC AGAGAGCACC GAGGACTTCT GGCGCATGCT CTGGGAGCAC 
GGCTCCACCA TCGTCGTCAT GCTGACCAGG CTTCGGGAGA TGGGCAGGGA GAAATGCCAC 
CAGTACTGGC CAGCAGAGCG CTCCGCTCGC TACCAATACT TTGTCGTTGA CCCGATGGCT 
GAGTACAACA TGCCGCAGTA CATCCTGCGT GAGTTCAAGG TCACAGATGC CCGGGATGGG 
CAGTCGAGGA CAATCCGGCA GTTCCAGTTC ACAGACTGGC CCGAGCAGGG CGTGCCTAAG 
ACGGGTGAGG GCTTCATCGA CTTCATCGGG CAGGTGCACA AGACCAAGGA GCAGTTCGGG 
CAGGACGGGC CCATCACGGT GCACTGCAGT GCCGGCGTGG GCCGCACCGG GGTGTTCATC 
ACTCTGAGCA TCGTCCTGGA GCGGATGCGC TACGAGGGCG TGGTCGACAT GTTCCAGACC 
GTGAAGACCC TGCGCACACA GCGCCCAGCC ATGGTGCAGA CGGAGGACCA GTACCAGCTG 
TGCTATCGTG CGGCCCTGGA GTACCTTGGC AGCTTTGACC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629168.1
CDS	125..5878
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPAASRNGVITQYSVAYEAVDGEDRGRHVVESISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQWRPEESEDYETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGSSVPSCPNISSMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_005629111.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629111.3

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCACCCCCTC AGCCCCTCCC CAGAAGGTGA CCTGTGTGAG CACGGGCTCC 
ACCACGGTTC GGGTAAGTTG GGTCCCGCCG CCAGCTGCCA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCAGTGGAC GGCGAGGACC GAGGGCGGCA TGTGGTGGAG 
AGCATCAGTC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACTGACGTG GGCCCCGGCC CAGAGAGCAG CCCGGTGCTC 
GTGCGCACAG ACGAGGACGT GCCCAGTGGA CCGCCACGGA AGGTGGAGGT GGAACCACTG 
AATTCCACTG CTGTGCGTGT CTCTTGGAAG CTGCCCGTCC CCAGCAAGCA GCACGGCCAG 
ATTCGCGGCT ACCAGGTCAC CTATGTGCGG CTAGAGAATG GCGAGCCCCG TGGCCCACCC 
ATCATCCAGG ACGTCATGCT GGCTGAGGCC CAGTGGCGGC CAGAGGAGTC CGAGGACTAT 
GAAACCATAA TCAGCGGCCT GACCCCAGAG ACCACCTATT CCATTACCGT CGCCGCCTAC 
ACCACCAAAG GGGATGGTGC CCGCAGCAAG CCCAAAGTTG TCACAACGAC CGGGGCAGTC 
CCAGGCCGGC CCACCATGAT GGTCAGCACC ACGGCCATGA ACACCGCACT GCTGCAGTGG 
CACCCCCCCA AGGAGCTGCC AGGGGAGCTG CTGGGCTACC GGCTACAGTA CCACCGTGCC 
GATGAGGCAA AGCCCAACAT CCTAGATTTT GGCAAGGACG ACCAGCACTT TACAGTCACC 
GGCCTACACA AGGGAGCCAC CTACATTTTC CGGCTTGCCG CCAAGAACCG GGCTGGCCCG 
GGAGAAGAGT TCGAGAAGGA GATCACGACT CCCGAGGATG CGCCCAGCGG CTTCCCCCAA 
AACCTGCGCG TGATAGGGCT GACCACCTCT ACCACGGAGC TGACCTGGGA CCCCCCGGTG 
CTGGCGGAGA GGAACGGGCG CATCACCAAC TACTCCGTGG TGTACCGTGA CATCAACAGC 
CAGCAGGAGC TACAGAACGT CACCGCCGAC ACCCACCTCA TGCTCTCCGG CCTCAAGCCG 
GACACCACTT ACGACATCAA GGTCCGCGCA CGTACCAGCA AGGGCGCCGG CCCACTCAGC 
CCCAGCATCC AGTCCCGGAC CATGCCCATG GAGCAAGTGT TTGCCAAGAA CTTCCGTGTG 
GCGGCCGCTA TGAAGACGTC TGTGCTGCTC AGCTGGGAGG TCCCAGACTC CTACAAGTCG 
GCTGTACCCT TCAAGATCCT GTACAACGGG CAGAGTGTGG AGGTGGACGG GCACTCCATG 
CGGAAACTGA TCGCAGACCT GCAGCCGAAC ACGGAGTACT CGTTCGTGCT GATGAACCGT 
GGCAGCAGCG CGGGGGGCCT GCAGCACCTC GTGTCCATCC GCACGGCCCC AGACCTCCTG 
CCACACAAGC CGATGCCCGC CTCCGCCTTC ATAGAGGATG GTCGCTTCAC CCTCTCCATG 
CCCCACGTGC AGGATGCCTC ACTCATCAGG TGGTTCTACA TCGTGGTGGT ACCCATCGAC 
CGCGTGGGTG GGAGTGTGCT GGCCCCGAGA TGGAGCACGC CTGAGGAGCT GGAGCTGGAC 
GAGCTTCTGG AGGCCATCGA GCAGGGCGGT GGGGAGCGGC GACGACGGCG GCAGGCGGAG 
CGGCTGAAGC CATATGTGGC TGCACAGGTG GACGTGCTCC CCGAGAGCTT CACCCTAGGA 
GACAAGAAGA GCTACCGGGG CTTCTACAAC CGGCCTCTGT CTCCGGACCT GAGCTATCAG 
TGCTTTGTGC TCGCCTCCCT GAAGGAACCC GTGGACCAGA AGCGCTATGC CTCCAGCCCC 
TACTCTGATG AGATCGTGGT CCAAGTGACA CCAGCCCAGC AGCAGGAGGA GCCTGAGATG 
CTGTGGGTGA CAGGCCCCGT CCTCGCAGTC ATCCTCATCA TCCTCATTGT CATCGCCATC 
CTCCTGTTCA AGAGGAAGAG GACTCACTCC CCATCATCCA AGGACGAGCA GTCAATCGGG 
CTGAAGGACT CCTTGCTGGC CCATTCCTCT GACCCTGTGG AGATGCGGAG GCTCAACTAC 
CAGACTCCAG GTTCCAGTGT CCCCAGTTGC CCGAATATCT CAAGTATGCG AGACCACCCG 
CCCATCCCCA TCACTGACCT GGCCGACAAC ATTGAGCGCC TCAAAGCCAA CGACGGCCTC 
AAGTTCTCCC AGGAGTACGA GTCCATTGAC CCTGGACAGC AGTTCACCTG GGAGAATTCA 
AACCTGGAAG TGAACAAGCC CAAGAACCGC TATGCAAACG TCATCGCCTA CGACCACTCC 
CGAGTCATCC TTACCTCCGT CGATGGTGTC CCAGGGAGTG ACTATATCAA CGCCAACTAC 
ATCGATGGCT ACCGCAAGCA GAACGCCTAC ATTGCCACGC AGGGCCCACT GCCCGAGACC 
ATGGGCGATT TCTGGCGGAT GGTGTGGGAA CAGCGCACAG CCACCGTGGT CATGATGACA 
CGGCTGGAGG AGAAGTCTCG GGTGAAGTGT GATCAGTACT GGCCAGCCCG GGGTACCGAG 
ACCCACGGCC TCATTCAGGT GACCCTGCTG GACACGGTGG AGCTGGCCAC ATACACTGTG 
CGCACCTTTG CACTCCACAA GAGTGGCTCC AGCGAGAAGC GGGAGCTGCG TCAGTTCCAG 
TTCATGGCCT GGCCGGACCA CGGGGTTCCC GAGTACCCCA CCCCCATCCT GGCCTTCCTG 
CGGCGGGTCA AGGCCTGTAA CCCGCTAGAC GCAGGGCCCA TGGTGGTGCA CTGCAGCGCG 
GGCGTGGGCC GCACGGGCTG TTTCATCGTC ATCGACGCCA TGCTGGAGCG GATGAAGCAC 
GAGAAGACAG TGGACATCTA TGGCCACGTG ACATGCATGC GATCGCAGCG GAACTACATG 
GTGCAGACAG AGGACCAGTA CGTGTTCATC CACGAGGCGC TGCTAGAGGC CGCCACGTGC 
GGACACACGG AGGTGCCTGC CCGCAGCCTG TATGCCCATA TCCAGAAGCT GGGCCAAGTG 
CCTCCCGGGG AGAGTGTGAC TGCCATGGAG CTCGAGTTCA AGCTGCTGGC CAGCTCCAAG 
GCCCACACAT CCCGCTTCAT TAGCGCCAAC CTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTGAACATCA TGCCCTATGA GTTGACCCGT GTGTGTCTGC AGCCCATCCG TGGTGTGGAG 
GGCTCTGACT ACATCAATGC CAGCTTCTTG GACGGCTATA GGCAGCAGAA GGCCTACATC 
GCCACGCAGG GGCCCCTGGC AGAGAGCACC GAGGACTTCT GGCGCATGCT CTGGGAGCAC 
GGCTCCACCA TCGTCGTCAT GCTGACCAGG CTTCGGGAGA TGGGCAGGGA GAAATGCCAC 
CAGTACTGGC CAGCAGAGCG CTCCGCTCGC TACCAATACT TTGTCGTTGA CCCGATGGCT 
GAGTACAACA TGCCGCAGTA CATCCTGCGT GAGTTCAAGG TCACAGATGC CCGGGATGGG 
CAGTCGAGGA CAATCCGGCA GTTCCAGTTC ACAGACTGGC CCGAGCAGGG CGTGCCTAAG 
ACGGGTGAGG GCTTCATCGA CTTCATCGGG CAGGTGCACA AGACCAAGGA GCAGTTCGGG 
CAGGACGGGC CCATCACGGT GCACTGCAGT GCCGGCGTGG GCCGCACCGG GGTGTTCATC 
ACTCTGAGCA TCGTCCTGGA GCGGATGCGC TACGAGGGCG TGGTCGACAT GTTCCAGACC 
GTGAAGACCC TGCGCACACA GCGCCCAGCC ATGGTGCAGA CGGAGGACCA GTACCAGCTG 
TGCTATCGTG CGGCCCTGGA GTACCTTGGC AGCTTTGACC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_005629112.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629169.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X3, mRNA.

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CloneID	OCe17421
Clone ID Related Accession (Same CDS sequence)	XM_005629112.2 , XM_005629112.3
Accession Version	XM_005629112.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629112.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GGAAACCATA 
ATCAGCGGCC TGACCCCAGA GACCACCTAT TCCATTACCG TCGCCGCCTA CACCACCAAA 
GGGGATGGTG CCCGCAGCAA GCCCAAAGTT GTCACAACGA CCGGGGCAGT CCCAGGCCGG 
CCCACCATGA TGGTCAGCAC CACGGCCATG AACACCGCAC TGCTGCAGTG GCACCCCCCC 
AAGGAGCTGC CAGGGGAGCT GCTGGGCTAC CGGCTACAGT ACCACCGTGC CGATGAGGCA 
AAGCCCAACA TCCTAGATTT TGGCAAGGAC GACCAGCACT TTACAGTCAC CGGCCTACAC 
AAGGGAGCCA CCTACATTTT CCGGCTTGCC GCCAAGAACC GGGCTGGCCC GGGAGAAGAG 
TTCGAGAAGG AGATCACGAC TCCCGAGGAT GCGCCCAGCG GCTTCCCCCA AAACCTGCGC 
GTGATAGGGC TGACCACCTC TACCACGGAG CTGACCTGGG ACCCCCCGGT GCTGGCGGAG 
AGGAACGGGC GCATCACCAA CTACTCCGTG GTGTACCGTG ACATCAACAG CCAGCAGGAG 
CTACAGAACG TCACCGCCGA CACCCACCTC ATGCTCTCCG GCCTCAAGCC GGACACCACT 
TACGACATCA AGGTCCGCGC ACGTACCAGC AAGGGCGCCG GCCCACTCAG CCCCAGCATC 
CAGTCCCGGA CCATGCCCAT GGAGCAAGTG TTTGCCAAGA ACTTCCGTGT GGCGGCCGCT 
ATGAAGACGT CTGTGCTGCT CAGCTGGGAG GTCCCAGACT CCTACAAGTC GGCTGTACCC 
TTCAAGATCC TGTACAACGG GCAGAGTGTG GAGGTGGACG GGCACTCCAT GCGGAAACTG 
ATCGCAGACC TGCAGCCGAA CACGGAGTAC TCGTTCGTGC TGATGAACCG TGGCAGCAGC 
GCGGGGGGCC TGCAGCACCT CGTGTCCATC CGCACGGCCC CAGACCTCCT GCCACACAAG 
CCGATGCCCG CCTCCGCCTT CATAGAGGAT GGTCGCTTCA CCCTCTCCAT GCCCCACGTG 
CAGGATGCCT CACTCATCAG GTGGTTCTAC ATCGTGGTGG TACCCATCGA CCGCGTGGGT 
GGGAGTGTGC TGGCCCCGAG ATGGAGCACG CCTGAGGAGC TGGAGCTGGA CGAGCTTCTG 
GAGGCCATCG AGCAGGGCGG TGGGGAGCGG CGACGACGGC GGCAGGCGGA GCGGCTGAAG 
CCATATGTGG CTGCACAGGT GGACGTGCTC CCCGAGAGCT TCACCCTAGG AGACAAGAAG 
AGCTACCGGG GCTTCTACAA CCGGCCTCTG TCTCCGGACC TGAGCTATCA GTGCTTTGTG 
CTCGCCTCCC TGAAGGAACC CGTGGACCAG AAGCGCTATG CCTCCAGCCC CTACTCTGAT 
GAGATCGTGG TCCAAGTGAC ACCAGCCCAG CAGCAGGAGG AGCCTGAGAT GCTGTGGGTG 
ACAGGCCCCG TCCTCGCAGT CATCCTCATC ATCCTCATTG TCATCGCCAT CCTCCTGTTC 
AAGAGGAAGA GGACTCACTC CCCATCATCC AAGGACGAGC AGTCAATCGG GCTGAAGGAC 
TCCTTGCTGG CCCATTCCTC TGACCCTGTG GAGATGCGGA GGCTCAACTA CCAGACTCCA 
GGTTCCAGTG TCCCCAGTTG CCCGAATATC TCAAGTATGC GAGACCACCC GCCCATCCCC 
ATCACTGACC TGGCCGACAA CATTGAGCGC CTCAAAGCCA ACGACGGCCT CAAGTTCTCC 
CAGGAGTACG AGTCCATTGA CCCTGGACAG CAGTTCACCT GGGAGAATTC AAACCTGGAA 
GTGAACAAGC CCAAGAACCG CTATGCAAAC GTCATCGCCT ACGACCACTC CCGAGTCATC 
CTTACCTCCG TCGATGGTGT CCCAGGGAGT GACTATATCA ACGCCAACTA CATCGATGGC 
TACCGCAAGC AGAACGCCTA CATTGCCACG CAGGGCCCAC TGCCCGAGAC CATGGGCGAT 
TTCTGGCGGA TGGTGTGGGA ACAGCGCACA GCCACCGTGG TCATGATGAC ACGGCTGGAG 
GAGAAGTCTC GGGTGAAGTG TGATCAGTAC TGGCCAGCCC GGGGTACCGA GACCCACGGC 
CTCATTCAGG TGACCCTGCT GGACACGGTG GAGCTGGCCA CATACACTGT GCGCACCTTT 
GCACTCCACA AGAGTGGCTC CAGCGAGAAG CGGGAGCTGC GTCAGTTCCA GTTCATGGCC 
TGGCCGGACC ACGGGGTTCC CGAGTACCCC ACCCCCATCC TGGCCTTCCT GCGGCGGGTC 
AAGGCCTGTA ACCCGCTAGA CGCAGGGCCC ATGGTGGTGC ACTGCAGCGC GGGCGTGGGC 
CGCACGGGCT GTTTCATCGT CATCGACGCC ATGCTGGAGC GGATGAAGCA CGAGAAGACA 
GTGGACATCT ATGGCCACGT GACATGCATG CGATCGCAGC GGAACTACAT GGTGCAGACA 
GAGGACCAGT ACGTGTTCAT CCACGAGGCG CTGCTAGAGG CCGCCACGTG CGGACACACG 
GAGGTGCCTG CCCGCAGCCT GTATGCCCAT ATCCAGAAGC TGGGCCAAGT GCCTCCCGGG 
GAGAGTGTGA CTGCCATGGA GCTCGAGTTC AAGCTGCTGG CCAGCTCCAA GGCCCACACA 
TCCCGCTTCA TTAGCGCCAA CCTGCCCTGC AACAAGTTCA AGAACCGCCT GGTGAACATC 
ATGCCCTATG AGTTGACCCG TGTGTGTCTG CAGCCCATCC GTGGTGTGGA GGGCTCTGAC 
TACATCAATG CCAGCTTCTT GGACGGCTAT AGGCAGCAGA AGGCCTACAT CGCCACGCAG 
GGGCCCCTGG CAGAGAGCAC CGAGGACTTC TGGCGCATGC TCTGGGAGCA CGGCTCCACC 
ATCGTCGTCA TGCTGACCAG GCTTCGGGAG ATGGGCAGGG AGAAATGCCA CCAGTACTGG 
CCAGCAGAGC GCTCCGCTCG CTACCAATAC TTTGTCGTTG ACCCGATGGC TGAGTACAAC 
ATGCCGCAGT ACATCCTGCG TGAGTTCAAG GTCACAGATG CCCGGGATGG GCAGTCGAGG 
ACAATCCGGC AGTTCCAGTT CACAGACTGG CCCGAGCAGG GCGTGCCTAA GACGGGTGAG 
GGCTTCATCG ACTTCATCGG GCAGGTGCAC AAGACCAAGG AGCAGTTCGG GCAGGACGGG 
CCCATCACGG TGCACTGCAG TGCCGGCGTG GGCCGCACCG GGGTGTTCAT CACTCTGAGC 
ATCGTCCTGG AGCGGATGCG CTACGAGGGC GTGGTCGACA TGTTCCAGAC CGTGAAGACC 
CTGCGCACAC AGCGCCCAGC CATGGTGCAG ACGGAGGACC AGTACCAGCT GTGCTATCGT 
GCGGCCCTGG AGTACCTTGG CAGCTTTGAC CACTATGCAA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629169.1
CDS	125..5869
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGGSPIRGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPAASRNGVITQYSVAYEAVDGEDRGRHVVESISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGSSVPSCPNISSMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_005629112.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629112.2

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GGAAACCATA 
ATCAGCGGCC TGACCCCAGA GACCACCTAT TCCATTACCG TCGCCGCCTA CACCACCAAA 
GGGGATGGTG CCCGCAGCAA GCCCAAAGTT GTCACAACGA CCGGGGCAGT CCCAGGCCGG 
CCCACCATGA TGGTCAGCAC CACGGCCATG AACACCGCAC TGCTGCAGTG GCACCCCCCC 
AAGGAGCTGC CAGGGGAGCT GCTGGGCTAC CGGCTACAGT ACCACCGTGC CGATGAGGCA 
AAGCCCAACA TCCTAGATTT TGGCAAGGAC GACCAGCACT TTACAGTCAC CGGCCTACAC 
AAGGGAGCCA CCTACATTTT CCGGCTTGCC GCCAAGAACC GGGCTGGCCC GGGAGAAGAG 
TTCGAGAAGG AGATCACGAC TCCCGAGGAT GCGCCCAGCG GCTTCCCCCA AAACCTGCGC 
GTGATAGGGC TGACCACCTC TACCACGGAG CTGACCTGGG ACCCCCCGGT GCTGGCGGAG 
AGGAACGGGC GCATCACCAA CTACTCCGTG GTGTACCGTG ACATCAACAG CCAGCAGGAG 
CTACAGAACG TCACCGCCGA CACCCACCTC ATGCTCTCCG GCCTCAAGCC GGACACCACT 
TACGACATCA AGGTCCGCGC ACGTACCAGC AAGGGCGCCG GCCCACTCAG CCCCAGCATC 
CAGTCCCGGA CCATGCCCAT GGAGCAAGTG TTTGCCAAGA ACTTCCGTGT GGCGGCCGCT 
ATGAAGACGT CTGTGCTGCT CAGCTGGGAG GTCCCAGACT CCTACAAGTC GGCTGTACCC 
TTCAAGATCC TGTACAACGG GCAGAGTGTG GAGGTGGACG GGCACTCCAT GCGGAAACTG 
ATCGCAGACC TGCAGCCGAA CACGGAGTAC TCGTTCGTGC TGATGAACCG TGGCAGCAGC 
GCGGGGGGCC TGCAGCACCT CGTGTCCATC CGCACGGCCC CAGACCTCCT GCCACACAAG 
CCGATGCCCG CCTCCGCCTT CATAGAGGAT GGTCGCTTCA CCCTCTCCAT GCCCCACGTG 
CAGGATGCCT CACTCATCAG GTGGTTCTAC ATCGTGGTGG TACCCATCGA CCGCGTGGGT 
GGGAGTGTGC TGGCCCCGAG ATGGAGCACG CCTGAGGAGC TGGAGCTGGA CGAGCTTCTG 
GAGGCCATCG AGCAGGGCGG TGGGGAGCGG CGACGACGGC GGCAGGCGGA GCGGCTGAAG 
CCATATGTGG CTGCACAGGT GGACGTGCTC CCCGAGAGCT TCACCCTAGG AGACAAGAAG 
AGCTACCGGG GCTTCTACAA CCGGCCTCTG TCTCCGGACC TGAGCTATCA GTGCTTTGTG 
CTCGCCTCCC TGAAGGAACC CGTGGACCAG AAGCGCTATG CCTCCAGCCC CTACTCTGAT 
GAGATCGTGG TCCAAGTGAC ACCAGCCCAG CAGCAGGAGG AGCCTGAGAT GCTGTGGGTG 
ACAGGCCCCG TCCTCGCAGT CATCCTCATC ATCCTCATTG TCATCGCCAT CCTCCTGTTC 
AAGAGGAAGA GGACTCACTC CCCATCATCC AAGGACGAGC AGTCAATCGG GCTGAAGGAC 
TCCTTGCTGG CCCATTCCTC TGACCCTGTG GAGATGCGGA GGCTCAACTA CCAGACTCCA 
GGTTCCAGTG TCCCCAGTTG CCCGAATATC TCAAGTATGC GAGACCACCC GCCCATCCCC 
ATCACTGACC TGGCCGACAA CATTGAGCGC CTCAAAGCCA ACGACGGCCT CAAGTTCTCC 
CAGGAGTACG AGTCCATTGA CCCTGGACAG CAGTTCACCT GGGAGAATTC AAACCTGGAA 
GTGAACAAGC CCAAGAACCG CTATGCAAAC GTCATCGCCT ACGACCACTC CCGAGTCATC 
CTTACCTCCG TCGATGGTGT CCCAGGGAGT GACTATATCA ACGCCAACTA CATCGATGGC 
TACCGCAAGC AGAACGCCTA CATTGCCACG CAGGGCCCAC TGCCCGAGAC CATGGGCGAT 
TTCTGGCGGA TGGTGTGGGA ACAGCGCACA GCCACCGTGG TCATGATGAC ACGGCTGGAG 
GAGAAGTCTC GGGTGAAGTG TGATCAGTAC TGGCCAGCCC GGGGTACCGA GACCCACGGC 
CTCATTCAGG TGACCCTGCT GGACACGGTG GAGCTGGCCA CATACACTGT GCGCACCTTT 
GCACTCCACA AGAGTGGCTC CAGCGAGAAG CGGGAGCTGC GTCAGTTCCA GTTCATGGCC 
TGGCCGGACC ACGGGGTTCC CGAGTACCCC ACCCCCATCC TGGCCTTCCT GCGGCGGGTC 
AAGGCCTGTA ACCCGCTAGA CGCAGGGCCC ATGGTGGTGC ACTGCAGCGC GGGCGTGGGC 
CGCACGGGCT GTTTCATCGT CATCGACGCC ATGCTGGAGC GGATGAAGCA CGAGAAGACA 
GTGGACATCT ATGGCCACGT GACATGCATG CGATCGCAGC GGAACTACAT GGTGCAGACA 
GAGGACCAGT ACGTGTTCAT CCACGAGGCG CTGCTAGAGG CCGCCACGTG CGGACACACG 
GAGGTGCCTG CCCGCAGCCT GTATGCCCAT ATCCAGAAGC TGGGCCAAGT GCCTCCCGGG 
GAGAGTGTGA CTGCCATGGA GCTCGAGTTC AAGCTGCTGG CCAGCTCCAA GGCCCACACA 
TCCCGCTTCA TTAGCGCCAA CCTGCCCTGC AACAAGTTCA AGAACCGCCT GGTGAACATC 
ATGCCCTATG AGTTGACCCG TGTGTGTCTG CAGCCCATCC GTGGTGTGGA GGGCTCTGAC 
TACATCAATG CCAGCTTCTT GGACGGCTAT AGGCAGCAGA AGGCCTACAT CGCCACGCAG 
GGGCCCCTGG CAGAGAGCAC CGAGGACTTC TGGCGCATGC TCTGGGAGCA CGGCTCCACC 
ATCGTCGTCA TGCTGACCAG GCTTCGGGAG ATGGGCAGGG AGAAATGCCA CCAGTACTGG 
CCAGCAGAGC GCTCCGCTCG CTACCAATAC TTTGTCGTTG ACCCGATGGC TGAGTACAAC 
ATGCCGCAGT ACATCCTGCG TGAGTTCAAG GTCACAGATG CCCGGGATGG GCAGTCGAGG 
ACAATCCGGC AGTTCCAGTT CACAGACTGG CCCGAGCAGG GCGTGCCTAA GACGGGTGAG 
GGCTTCATCG ACTTCATCGG GCAGGTGCAC AAGACCAAGG AGCAGTTCGG GCAGGACGGG 
CCCATCACGG TGCACTGCAG TGCCGGCGTG GGCCGCACCG GGGTGTTCAT CACTCTGAGC 
ATCGTCCTGG AGCGGATGCG CTACGAGGGC GTGGTCGACA TGTTCCAGAC CGTGAAGACC 
CTGCGCACAC AGCGCCCAGC CATGGTGCAG ACGGAGGACC AGTACCAGCT GTGCTATCGT 
GCGGCCCTGG AGTACCTTGG CAGCTTTGAC CACTATGCAA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_005629112.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629169.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X3, mRNA.

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CloneID	OCe17421
Clone ID Related Accession (Same CDS sequence)	XM_005629112.2 , XM_005629112.3
Accession Version	XM_005629112.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629112.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GGAAACCATA 
ATCAGCGGCC TGACCCCAGA GACCACCTAT TCCATTACCG TCGCCGCCTA CACCACCAAA 
GGGGATGGTG CCCGCAGCAA GCCCAAAGTT GTCACAACGA CCGGGGCAGT CCCAGGCCGG 
CCCACCATGA TGGTCAGCAC CACGGCCATG AACACCGCAC TGCTGCAGTG GCACCCCCCC 
AAGGAGCTGC CAGGGGAGCT GCTGGGCTAC CGGCTACAGT ACCACCGTGC CGATGAGGCA 
AAGCCCAACA TCCTAGATTT TGGCAAGGAC GACCAGCACT TTACAGTCAC CGGCCTACAC 
AAGGGAGCCA CCTACATTTT CCGGCTTGCC GCCAAGAACC GGGCTGGCCC GGGAGAAGAG 
TTCGAGAAGG AGATCACGAC TCCCGAGGAT GCGCCCAGCG GCTTCCCCCA AAACCTGCGC 
GTGATAGGGC TGACCACCTC TACCACGGAG CTGACCTGGG ACCCCCCGGT GCTGGCGGAG 
AGGAACGGGC GCATCACCAA CTACTCCGTG GTGTACCGTG ACATCAACAG CCAGCAGGAG 
CTACAGAACG TCACCGCCGA CACCCACCTC ATGCTCTCCG GCCTCAAGCC GGACACCACT 
TACGACATCA AGGTCCGCGC ACGTACCAGC AAGGGCGCCG GCCCACTCAG CCCCAGCATC 
CAGTCCCGGA CCATGCCCAT GGAGCAAGTG TTTGCCAAGA ACTTCCGTGT GGCGGCCGCT 
ATGAAGACGT CTGTGCTGCT CAGCTGGGAG GTCCCAGACT CCTACAAGTC GGCTGTACCC 
TTCAAGATCC TGTACAACGG GCAGAGTGTG GAGGTGGACG GGCACTCCAT GCGGAAACTG 
ATCGCAGACC TGCAGCCGAA CACGGAGTAC TCGTTCGTGC TGATGAACCG TGGCAGCAGC 
GCGGGGGGCC TGCAGCACCT CGTGTCCATC CGCACGGCCC CAGACCTCCT GCCACACAAG 
CCGATGCCCG CCTCCGCCTT CATAGAGGAT GGTCGCTTCA CCCTCTCCAT GCCCCACGTG 
CAGGATGCCT CACTCATCAG GTGGTTCTAC ATCGTGGTGG TACCCATCGA CCGCGTGGGT 
GGGAGTGTGC TGGCCCCGAG ATGGAGCACG CCTGAGGAGC TGGAGCTGGA CGAGCTTCTG 
GAGGCCATCG AGCAGGGCGG TGGGGAGCGG CGACGACGGC GGCAGGCGGA GCGGCTGAAG 
CCATATGTGG CTGCACAGGT GGACGTGCTC CCCGAGAGCT TCACCCTAGG AGACAAGAAG 
AGCTACCGGG GCTTCTACAA CCGGCCTCTG TCTCCGGACC TGAGCTATCA GTGCTTTGTG 
CTCGCCTCCC TGAAGGAACC CGTGGACCAG AAGCGCTATG CCTCCAGCCC CTACTCTGAT 
GAGATCGTGG TCCAAGTGAC ACCAGCCCAG CAGCAGGAGG AGCCTGAGAT GCTGTGGGTG 
ACAGGCCCCG TCCTCGCAGT CATCCTCATC ATCCTCATTG TCATCGCCAT CCTCCTGTTC 
AAGAGGAAGA GGACTCACTC CCCATCATCC AAGGACGAGC AGTCAATCGG GCTGAAGGAC 
TCCTTGCTGG CCCATTCCTC TGACCCTGTG GAGATGCGGA GGCTCAACTA CCAGACTCCA 
GGTTCCAGTG TCCCCAGTTG CCCGAATATC TCAAGTATGC GAGACCACCC GCCCATCCCC 
ATCACTGACC TGGCCGACAA CATTGAGCGC CTCAAAGCCA ACGACGGCCT CAAGTTCTCC 
CAGGAGTACG AGTCCATTGA CCCTGGACAG CAGTTCACCT GGGAGAATTC AAACCTGGAA 
GTGAACAAGC CCAAGAACCG CTATGCAAAC GTCATCGCCT ACGACCACTC CCGAGTCATC 
CTTACCTCCG TCGATGGTGT CCCAGGGAGT GACTATATCA ACGCCAACTA CATCGATGGC 
TACCGCAAGC AGAACGCCTA CATTGCCACG CAGGGCCCAC TGCCCGAGAC CATGGGCGAT 
TTCTGGCGGA TGGTGTGGGA ACAGCGCACA GCCACCGTGG TCATGATGAC ACGGCTGGAG 
GAGAAGTCTC GGGTGAAGTG TGATCAGTAC TGGCCAGCCC GGGGTACCGA GACCCACGGC 
CTCATTCAGG TGACCCTGCT GGACACGGTG GAGCTGGCCA CATACACTGT GCGCACCTTT 
GCACTCCACA AGAGTGGCTC CAGCGAGAAG CGGGAGCTGC GTCAGTTCCA GTTCATGGCC 
TGGCCGGACC ACGGGGTTCC CGAGTACCCC ACCCCCATCC TGGCCTTCCT GCGGCGGGTC 
AAGGCCTGTA ACCCGCTAGA CGCAGGGCCC ATGGTGGTGC ACTGCAGCGC GGGCGTGGGC 
CGCACGGGCT GTTTCATCGT CATCGACGCC ATGCTGGAGC GGATGAAGCA CGAGAAGACA 
GTGGACATCT ATGGCCACGT GACATGCATG CGATCGCAGC GGAACTACAT GGTGCAGACA 
GAGGACCAGT ACGTGTTCAT CCACGAGGCG CTGCTAGAGG CCGCCACGTG CGGACACACG 
GAGGTGCCTG CCCGCAGCCT GTATGCCCAT ATCCAGAAGC TGGGCCAAGT GCCTCCCGGG 
GAGAGTGTGA CTGCCATGGA GCTCGAGTTC AAGCTGCTGG CCAGCTCCAA GGCCCACACA 
TCCCGCTTCA TTAGCGCCAA CCTGCCCTGC AACAAGTTCA AGAACCGCCT GGTGAACATC 
ATGCCCTATG AGTTGACCCG TGTGTGTCTG CAGCCCATCC GTGGTGTGGA GGGCTCTGAC 
TACATCAATG CCAGCTTCTT GGACGGCTAT AGGCAGCAGA AGGCCTACAT CGCCACGCAG 
GGGCCCCTGG CAGAGAGCAC CGAGGACTTC TGGCGCATGC TCTGGGAGCA CGGCTCCACC 
ATCGTCGTCA TGCTGACCAG GCTTCGGGAG ATGGGCAGGG AGAAATGCCA CCAGTACTGG 
CCAGCAGAGC GCTCCGCTCG CTACCAATAC TTTGTCGTTG ACCCGATGGC TGAGTACAAC 
ATGCCGCAGT ACATCCTGCG TGAGTTCAAG GTCACAGATG CCCGGGATGG GCAGTCGAGG 
ACAATCCGGC AGTTCCAGTT CACAGACTGG CCCGAGCAGG GCGTGCCTAA GACGGGTGAG 
GGCTTCATCG ACTTCATCGG GCAGGTGCAC AAGACCAAGG AGCAGTTCGG GCAGGACGGG 
CCCATCACGG TGCACTGCAG TGCCGGCGTG GGCCGCACCG GGGTGTTCAT CACTCTGAGC 
ATCGTCCTGG AGCGGATGCG CTACGAGGGC GTGGTCGACA TGTTCCAGAC CGTGAAGACC 
CTGCGCACAC AGCGCCCAGC CATGGTGCAG ACGGAGGACC AGTACCAGCT GTGCTATCGT 
GCGGCCCTGG AGTACCTTGG CAGCTTTGAC CACTATGCAA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629169.1
CDS	125..5869
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGGSPIRGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPAASRNGVITQYSVAYEAVDGEDRGRHVVESISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGSSVPSCPNISSMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_005629112.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629112.3

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GGAAACCATA 
ATCAGCGGCC TGACCCCAGA GACCACCTAT TCCATTACCG TCGCCGCCTA CACCACCAAA 
GGGGATGGTG CCCGCAGCAA GCCCAAAGTT GTCACAACGA CCGGGGCAGT CCCAGGCCGG 
CCCACCATGA TGGTCAGCAC CACGGCCATG AACACCGCAC TGCTGCAGTG GCACCCCCCC 
AAGGAGCTGC CAGGGGAGCT GCTGGGCTAC CGGCTACAGT ACCACCGTGC CGATGAGGCA 
AAGCCCAACA TCCTAGATTT TGGCAAGGAC GACCAGCACT TTACAGTCAC CGGCCTACAC 
AAGGGAGCCA CCTACATTTT CCGGCTTGCC GCCAAGAACC GGGCTGGCCC GGGAGAAGAG 
TTCGAGAAGG AGATCACGAC TCCCGAGGAT GCGCCCAGCG GCTTCCCCCA AAACCTGCGC 
GTGATAGGGC TGACCACCTC TACCACGGAG CTGACCTGGG ACCCCCCGGT GCTGGCGGAG 
AGGAACGGGC GCATCACCAA CTACTCCGTG GTGTACCGTG ACATCAACAG CCAGCAGGAG 
CTACAGAACG TCACCGCCGA CACCCACCTC ATGCTCTCCG GCCTCAAGCC GGACACCACT 
TACGACATCA AGGTCCGCGC ACGTACCAGC AAGGGCGCCG GCCCACTCAG CCCCAGCATC 
CAGTCCCGGA CCATGCCCAT GGAGCAAGTG TTTGCCAAGA ACTTCCGTGT GGCGGCCGCT 
ATGAAGACGT CTGTGCTGCT CAGCTGGGAG GTCCCAGACT CCTACAAGTC GGCTGTACCC 
TTCAAGATCC TGTACAACGG GCAGAGTGTG GAGGTGGACG GGCACTCCAT GCGGAAACTG 
ATCGCAGACC TGCAGCCGAA CACGGAGTAC TCGTTCGTGC TGATGAACCG TGGCAGCAGC 
GCGGGGGGCC TGCAGCACCT CGTGTCCATC CGCACGGCCC CAGACCTCCT GCCACACAAG 
CCGATGCCCG CCTCCGCCTT CATAGAGGAT GGTCGCTTCA CCCTCTCCAT GCCCCACGTG 
CAGGATGCCT CACTCATCAG GTGGTTCTAC ATCGTGGTGG TACCCATCGA CCGCGTGGGT 
GGGAGTGTGC TGGCCCCGAG ATGGAGCACG CCTGAGGAGC TGGAGCTGGA CGAGCTTCTG 
GAGGCCATCG AGCAGGGCGG TGGGGAGCGG CGACGACGGC GGCAGGCGGA GCGGCTGAAG 
CCATATGTGG CTGCACAGGT GGACGTGCTC CCCGAGAGCT TCACCCTAGG AGACAAGAAG 
AGCTACCGGG GCTTCTACAA CCGGCCTCTG TCTCCGGACC TGAGCTATCA GTGCTTTGTG 
CTCGCCTCCC TGAAGGAACC CGTGGACCAG AAGCGCTATG CCTCCAGCCC CTACTCTGAT 
GAGATCGTGG TCCAAGTGAC ACCAGCCCAG CAGCAGGAGG AGCCTGAGAT GCTGTGGGTG 
ACAGGCCCCG TCCTCGCAGT CATCCTCATC ATCCTCATTG TCATCGCCAT CCTCCTGTTC 
AAGAGGAAGA GGACTCACTC CCCATCATCC AAGGACGAGC AGTCAATCGG GCTGAAGGAC 
TCCTTGCTGG CCCATTCCTC TGACCCTGTG GAGATGCGGA GGCTCAACTA CCAGACTCCA 
GGTTCCAGTG TCCCCAGTTG CCCGAATATC TCAAGTATGC GAGACCACCC GCCCATCCCC 
ATCACTGACC TGGCCGACAA CATTGAGCGC CTCAAAGCCA ACGACGGCCT CAAGTTCTCC 
CAGGAGTACG AGTCCATTGA CCCTGGACAG CAGTTCACCT GGGAGAATTC AAACCTGGAA 
GTGAACAAGC CCAAGAACCG CTATGCAAAC GTCATCGCCT ACGACCACTC CCGAGTCATC 
CTTACCTCCG TCGATGGTGT CCCAGGGAGT GACTATATCA ACGCCAACTA CATCGATGGC 
TACCGCAAGC AGAACGCCTA CATTGCCACG CAGGGCCCAC TGCCCGAGAC CATGGGCGAT 
TTCTGGCGGA TGGTGTGGGA ACAGCGCACA GCCACCGTGG TCATGATGAC ACGGCTGGAG 
GAGAAGTCTC GGGTGAAGTG TGATCAGTAC TGGCCAGCCC GGGGTACCGA GACCCACGGC 
CTCATTCAGG TGACCCTGCT GGACACGGTG GAGCTGGCCA CATACACTGT GCGCACCTTT 
GCACTCCACA AGAGTGGCTC CAGCGAGAAG CGGGAGCTGC GTCAGTTCCA GTTCATGGCC 
TGGCCGGACC ACGGGGTTCC CGAGTACCCC ACCCCCATCC TGGCCTTCCT GCGGCGGGTC 
AAGGCCTGTA ACCCGCTAGA CGCAGGGCCC ATGGTGGTGC ACTGCAGCGC GGGCGTGGGC 
CGCACGGGCT GTTTCATCGT CATCGACGCC ATGCTGGAGC GGATGAAGCA CGAGAAGACA 
GTGGACATCT ATGGCCACGT GACATGCATG CGATCGCAGC GGAACTACAT GGTGCAGACA 
GAGGACCAGT ACGTGTTCAT CCACGAGGCG CTGCTAGAGG CCGCCACGTG CGGACACACG 
GAGGTGCCTG CCCGCAGCCT GTATGCCCAT ATCCAGAAGC TGGGCCAAGT GCCTCCCGGG 
GAGAGTGTGA CTGCCATGGA GCTCGAGTTC AAGCTGCTGG CCAGCTCCAA GGCCCACACA 
TCCCGCTTCA TTAGCGCCAA CCTGCCCTGC AACAAGTTCA AGAACCGCCT GGTGAACATC 
ATGCCCTATG AGTTGACCCG TGTGTGTCTG CAGCCCATCC GTGGTGTGGA GGGCTCTGAC 
TACATCAATG CCAGCTTCTT GGACGGCTAT AGGCAGCAGA AGGCCTACAT CGCCACGCAG 
GGGCCCCTGG CAGAGAGCAC CGAGGACTTC TGGCGCATGC TCTGGGAGCA CGGCTCCACC 
ATCGTCGTCA TGCTGACCAG GCTTCGGGAG ATGGGCAGGG AGAAATGCCA CCAGTACTGG 
CCAGCAGAGC GCTCCGCTCG CTACCAATAC TTTGTCGTTG ACCCGATGGC TGAGTACAAC 
ATGCCGCAGT ACATCCTGCG TGAGTTCAAG GTCACAGATG CCCGGGATGG GCAGTCGAGG 
ACAATCCGGC AGTTCCAGTT CACAGACTGG CCCGAGCAGG GCGTGCCTAA GACGGGTGAG 
GGCTTCATCG ACTTCATCGG GCAGGTGCAC AAGACCAAGG AGCAGTTCGG GCAGGACGGG 
CCCATCACGG TGCACTGCAG TGCCGGCGTG GGCCGCACCG GGGTGTTCAT CACTCTGAGC 
ATCGTCCTGG AGCGGATGCG CTACGAGGGC GTGGTCGACA TGTTCCAGAC CGTGAAGACC 
CTGCGCACAC AGCGCCCAGC CATGGTGCAG ACGGAGGACC AGTACCAGCT GTGCTATCGT 
GCGGCCCTGG AGTACCTTGG CAGCTTTGAC CACTATGCAA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_005629113.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629170.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X4, mRNA.

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CloneID	OCe17422
Clone ID Related Accession (Same CDS sequence)	XM_005629113.2 , XM_005629113.3
Accession Version	XM_005629113.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GTGGCGGCCA 
GAGGAGTCCG AGGACTATGA AACCATAATC AGCGGCCTGA CCCCAGAGAC CACCTATTCC 
ATTACCGTCG CCGCCTACAC CACCAAAGGG GATGGTGCCC GCAGCAAGCC CAAAGTTGTC 
ACAACGACCG GGGCAGTCCC AGGCCGGCCC ACCATGATGG TCAGCACCAC GGCCATGAAC 
ACCGCACTGC TGCAGTGGCA CCCCCCCAAG GAGCTGCCAG GGGAGCTGCT GGGCTACCGG 
CTACAGTACC ACCGTGCCGA TGAGGCAAAG CCCAACATCC TAGATTTTGG CAAGGACGAC 
CAGCACTTTA CAGTCACCGG CCTACACAAG GGAGCCACCT ACATTTTCCG GCTTGCCGCC 
AAGAACCGGG CTGGCCCGGG AGAAGAGTTC GAGAAGGAGA TCACGACTCC CGAGGATGCG 
CCCAGCGGCT TCCCCCAAAA CCTGCGCGTG ATAGGGCTGA CCACCTCTAC CACGGAGCTG 
ACCTGGGACC CCCCGGTGCT GGCGGAGAGG AACGGGCGCA TCACCAACTA CTCCGTGGTG 
TACCGTGACA TCAACAGCCA GCAGGAGCTA CAGAACGTCA CCGCCGACAC CCACCTCATG 
CTCTCCGGCC TCAAGCCGGA CACCACTTAC GACATCAAGG TCCGCGCACG TACCAGCAAG 
GGCGCCGGCC CACTCAGCCC CAGCATCCAG TCCCGGACCA TGCCCATGGA GCAAGTGTTT 
GCCAAGAACT TCCGTGTGGC GGCCGCTATG AAGACGTCTG TGCTGCTCAG CTGGGAGGTC 
CCAGACTCCT ACAAGTCGGC TGTACCCTTC AAGATCCTGT ACAACGGGCA GAGTGTGGAG 
GTGGACGGGC ACTCCATGCG GAAACTGATC GCAGACCTGC AGCCGAACAC GGAGTACTCG 
TTCGTGCTGA TGAACCGTGG CAGCAGCGCG GGGGGCCTGC AGCACCTCGT GTCCATCCGC 
ACGGCCCCAG ACCTCCTGCC ACACAAGCCG ATGCCCGCCT CCGCCTTCAT AGAGGATGGT 
CGCTTCACCC TCTCCATGCC CCACGTGCAG GATGCCTCAC TCATCAGGTG GTTCTACATC 
GTGGTGGTAC CCATCGACCG CGTGGGTGGG AGTGTGCTGG CCCCGAGATG GAGCACGCCT 
GAGGAGCTGG AGCTGGACGA GCTTCTGGAG GCCATCGAGC AGGGCGGTGG GGAGCGGCGA 
CGACGGCGGC AGGCGGAGCG GCTGAAGCCA TATGTGGCTG CACAGGTGGA CGTGCTCCCC 
GAGAGCTTCA CCCTAGGAGA CAAGAAGAGC TACCGGGGCT TCTACAACCG GCCTCTGTCT 
CCGGACCTGA GCTATCAGTG CTTTGTGCTC GCCTCCCTGA AGGAACCCGT GGACCAGAAG 
CGCTATGCCT CCAGCCCCTA CTCTGATGAG ATCGTGGTCC AAGTGACACC AGCCCAGCAG 
CAGGAGGAGC CTGAGATGCT GTGGGTGACA GGCCCCGTCC TCGCAGTCAT CCTCATCATC 
CTCATTGTCA TCGCCATCCT CCTGTTCAAG AGGAAGAGGA CTCACTCCCC ATCATCCAAG 
GACGAGCAGT CAATCGGGCT GAAGGACTCC TTGCTGGCCC ATTCCTCTGA CCCTGTGGAG 
ATGCGGAGGC TCAACTACCA GACTCCAGGT ATGCGAGACC ACCCGCCCAT CCCCATCACT 
GACCTGGCCG ACAACATTGA GCGCCTCAAA GCCAACGACG GCCTCAAGTT CTCCCAGGAG 
TACGAGTCCA TTGACCCTGG ACAGCAGTTC ACCTGGGAGA ATTCAAACCT GGAAGTGAAC 
AAGCCCAAGA ACCGCTATGC AAACGTCATC GCCTACGACC ACTCCCGAGT CATCCTTACC 
TCCGTCGATG GTGTCCCAGG GAGTGACTAT ATCAACGCCA ACTACATCGA TGGCTACCGC 
AAGCAGAACG CCTACATTGC CACGCAGGGC CCACTGCCCG AGACCATGGG CGATTTCTGG 
CGGATGGTGT GGGAACAGCG CACAGCCACC GTGGTCATGA TGACACGGCT GGAGGAGAAG 
TCTCGGGTGA AGTGTGATCA GTACTGGCCA GCCCGGGGTA CCGAGACCCA CGGCCTCATT 
CAGGTGACCC TGCTGGACAC GGTGGAGCTG GCCACATACA CTGTGCGCAC CTTTGCACTC 
CACAAGAGTG GCTCCAGCGA GAAGCGGGAG CTGCGTCAGT TCCAGTTCAT GGCCTGGCCG 
GACCACGGGG TTCCCGAGTA CCCCACCCCC ATCCTGGCCT TCCTGCGGCG GGTCAAGGCC 
TGTAACCCGC TAGACGCAGG GCCCATGGTG GTGCACTGCA GCGCGGGCGT GGGCCGCACG 
GGCTGTTTCA TCGTCATCGA CGCCATGCTG GAGCGGATGA AGCACGAGAA GACAGTGGAC 
ATCTATGGCC ACGTGACATG CATGCGATCG CAGCGGAACT ACATGGTGCA GACAGAGGAC 
CAGTACGTGT TCATCCACGA GGCGCTGCTA GAGGCCGCCA CGTGCGGACA CACGGAGGTG 
CCTGCCCGCA GCCTGTATGC CCATATCCAG AAGCTGGGCC AAGTGCCTCC CGGGGAGAGT 
GTGACTGCCA TGGAGCTCGA GTTCAAGCTG CTGGCCAGCT CCAAGGCCCA CACATCCCGC 
TTCATTAGCG CCAACCTGCC CTGCAACAAG TTCAAGAACC GCCTGGTGAA CATCATGCCC 
TATGAGTTGA CCCGTGTGTG TCTGCAGCCC ATCCGTGGTG TGGAGGGCTC TGACTACATC 
AATGCCAGCT TCTTGGACGG CTATAGGCAG CAGAAGGCCT ACATCGCCAC GCAGGGGCCC 
CTGGCAGAGA GCACCGAGGA CTTCTGGCGC ATGCTCTGGG AGCACGGCTC CACCATCGTC 
GTCATGCTGA CCAGGCTTCG GGAGATGGGC AGGGAGAAAT GCCACCAGTA CTGGCCAGCA 
GAGCGCTCCG CTCGCTACCA ATACTTTGTC GTTGACCCGA TGGCTGAGTA CAACATGCCG 
CAGTACATCC TGCGTGAGTT CAAGGTCACA GATGCCCGGG ATGGGCAGTC GAGGACAATC 
CGGCAGTTCC AGTTCACAGA CTGGCCCGAG CAGGGCGTGC CTAAGACGGG TGAGGGCTTC 
ATCGACTTCA TCGGGCAGGT GCACAAGACC AAGGAGCAGT TCGGGCAGGA CGGGCCCATC 
ACGGTGCACT GCAGTGCCGG CGTGGGCCGC ACCGGGGTGT TCATCACTCT GAGCATCGTC 
CTGGAGCGGA TGCGCTACGA GGGCGTGGTC GACATGTTCC AGACCGTGAA GACCCTGCGC 
ACACAGCGCC CAGCCATGGT GCAGACGGAG GACCAGTACC AGCTGTGCTA TCGTGCGGCC 
CTGGAGTACC TTGGCAGCTT TGACCACTAT GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629170.1
CDS	125..5863
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGGSPIRGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPAASRNGVITQYSVAYEAVDGEDRGRHVVESISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQWRPEESEDYETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_005629113.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629113.2

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GTGGCGGCCA 
GAGGAGTCCG AGGACTATGA AACCATAATC AGCGGCCTGA CCCCAGAGAC CACCTATTCC 
ATTACCGTCG CCGCCTACAC CACCAAAGGG GATGGTGCCC GCAGCAAGCC CAAAGTTGTC 
ACAACGACCG GGGCAGTCCC AGGCCGGCCC ACCATGATGG TCAGCACCAC GGCCATGAAC 
ACCGCACTGC TGCAGTGGCA CCCCCCCAAG GAGCTGCCAG GGGAGCTGCT GGGCTACCGG 
CTACAGTACC ACCGTGCCGA TGAGGCAAAG CCCAACATCC TAGATTTTGG CAAGGACGAC 
CAGCACTTTA CAGTCACCGG CCTACACAAG GGAGCCACCT ACATTTTCCG GCTTGCCGCC 
AAGAACCGGG CTGGCCCGGG AGAAGAGTTC GAGAAGGAGA TCACGACTCC CGAGGATGCG 
CCCAGCGGCT TCCCCCAAAA CCTGCGCGTG ATAGGGCTGA CCACCTCTAC CACGGAGCTG 
ACCTGGGACC CCCCGGTGCT GGCGGAGAGG AACGGGCGCA TCACCAACTA CTCCGTGGTG 
TACCGTGACA TCAACAGCCA GCAGGAGCTA CAGAACGTCA CCGCCGACAC CCACCTCATG 
CTCTCCGGCC TCAAGCCGGA CACCACTTAC GACATCAAGG TCCGCGCACG TACCAGCAAG 
GGCGCCGGCC CACTCAGCCC CAGCATCCAG TCCCGGACCA TGCCCATGGA GCAAGTGTTT 
GCCAAGAACT TCCGTGTGGC GGCCGCTATG AAGACGTCTG TGCTGCTCAG CTGGGAGGTC 
CCAGACTCCT ACAAGTCGGC TGTACCCTTC AAGATCCTGT ACAACGGGCA GAGTGTGGAG 
GTGGACGGGC ACTCCATGCG GAAACTGATC GCAGACCTGC AGCCGAACAC GGAGTACTCG 
TTCGTGCTGA TGAACCGTGG CAGCAGCGCG GGGGGCCTGC AGCACCTCGT GTCCATCCGC 
ACGGCCCCAG ACCTCCTGCC ACACAAGCCG ATGCCCGCCT CCGCCTTCAT AGAGGATGGT 
CGCTTCACCC TCTCCATGCC CCACGTGCAG GATGCCTCAC TCATCAGGTG GTTCTACATC 
GTGGTGGTAC CCATCGACCG CGTGGGTGGG AGTGTGCTGG CCCCGAGATG GAGCACGCCT 
GAGGAGCTGG AGCTGGACGA GCTTCTGGAG GCCATCGAGC AGGGCGGTGG GGAGCGGCGA 
CGACGGCGGC AGGCGGAGCG GCTGAAGCCA TATGTGGCTG CACAGGTGGA CGTGCTCCCC 
GAGAGCTTCA CCCTAGGAGA CAAGAAGAGC TACCGGGGCT TCTACAACCG GCCTCTGTCT 
CCGGACCTGA GCTATCAGTG CTTTGTGCTC GCCTCCCTGA AGGAACCCGT GGACCAGAAG 
CGCTATGCCT CCAGCCCCTA CTCTGATGAG ATCGTGGTCC AAGTGACACC AGCCCAGCAG 
CAGGAGGAGC CTGAGATGCT GTGGGTGACA GGCCCCGTCC TCGCAGTCAT CCTCATCATC 
CTCATTGTCA TCGCCATCCT CCTGTTCAAG AGGAAGAGGA CTCACTCCCC ATCATCCAAG 
GACGAGCAGT CAATCGGGCT GAAGGACTCC TTGCTGGCCC ATTCCTCTGA CCCTGTGGAG 
ATGCGGAGGC TCAACTACCA GACTCCAGGT ATGCGAGACC ACCCGCCCAT CCCCATCACT 
GACCTGGCCG ACAACATTGA GCGCCTCAAA GCCAACGACG GCCTCAAGTT CTCCCAGGAG 
TACGAGTCCA TTGACCCTGG ACAGCAGTTC ACCTGGGAGA ATTCAAACCT GGAAGTGAAC 
AAGCCCAAGA ACCGCTATGC AAACGTCATC GCCTACGACC ACTCCCGAGT CATCCTTACC 
TCCGTCGATG GTGTCCCAGG GAGTGACTAT ATCAACGCCA ACTACATCGA TGGCTACCGC 
AAGCAGAACG CCTACATTGC CACGCAGGGC CCACTGCCCG AGACCATGGG CGATTTCTGG 
CGGATGGTGT GGGAACAGCG CACAGCCACC GTGGTCATGA TGACACGGCT GGAGGAGAAG 
TCTCGGGTGA AGTGTGATCA GTACTGGCCA GCCCGGGGTA CCGAGACCCA CGGCCTCATT 
CAGGTGACCC TGCTGGACAC GGTGGAGCTG GCCACATACA CTGTGCGCAC CTTTGCACTC 
CACAAGAGTG GCTCCAGCGA GAAGCGGGAG CTGCGTCAGT TCCAGTTCAT GGCCTGGCCG 
GACCACGGGG TTCCCGAGTA CCCCACCCCC ATCCTGGCCT TCCTGCGGCG GGTCAAGGCC 
TGTAACCCGC TAGACGCAGG GCCCATGGTG GTGCACTGCA GCGCGGGCGT GGGCCGCACG 
GGCTGTTTCA TCGTCATCGA CGCCATGCTG GAGCGGATGA AGCACGAGAA GACAGTGGAC 
ATCTATGGCC ACGTGACATG CATGCGATCG CAGCGGAACT ACATGGTGCA GACAGAGGAC 
CAGTACGTGT TCATCCACGA GGCGCTGCTA GAGGCCGCCA CGTGCGGACA CACGGAGGTG 
CCTGCCCGCA GCCTGTATGC CCATATCCAG AAGCTGGGCC AAGTGCCTCC CGGGGAGAGT 
GTGACTGCCA TGGAGCTCGA GTTCAAGCTG CTGGCCAGCT CCAAGGCCCA CACATCCCGC 
TTCATTAGCG CCAACCTGCC CTGCAACAAG TTCAAGAACC GCCTGGTGAA CATCATGCCC 
TATGAGTTGA CCCGTGTGTG TCTGCAGCCC ATCCGTGGTG TGGAGGGCTC TGACTACATC 
AATGCCAGCT TCTTGGACGG CTATAGGCAG CAGAAGGCCT ACATCGCCAC GCAGGGGCCC 
CTGGCAGAGA GCACCGAGGA CTTCTGGCGC ATGCTCTGGG AGCACGGCTC CACCATCGTC 
GTCATGCTGA CCAGGCTTCG GGAGATGGGC AGGGAGAAAT GCCACCAGTA CTGGCCAGCA 
GAGCGCTCCG CTCGCTACCA ATACTTTGTC GTTGACCCGA TGGCTGAGTA CAACATGCCG 
CAGTACATCC TGCGTGAGTT CAAGGTCACA GATGCCCGGG ATGGGCAGTC GAGGACAATC 
CGGCAGTTCC AGTTCACAGA CTGGCCCGAG CAGGGCGTGC CTAAGACGGG TGAGGGCTTC 
ATCGACTTCA TCGGGCAGGT GCACAAGACC AAGGAGCAGT TCGGGCAGGA CGGGCCCATC 
ACGGTGCACT GCAGTGCCGG CGTGGGCCGC ACCGGGGTGT TCATCACTCT GAGCATCGTC 
CTGGAGCGGA TGCGCTACGA GGGCGTGGTC GACATGTTCC AGACCGTGAA GACCCTGCGC 
ACACAGCGCC CAGCCATGGT GCAGACGGAG GACCAGTACC AGCTGTGCTA TCGTGCGGCC 
CTGGAGTACC TTGGCAGCTT TGACCACTAT GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_005629113.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629170.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X4, mRNA.

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CloneID	OCe17422
Clone ID Related Accession (Same CDS sequence)	XM_005629113.2 , XM_005629113.3
Accession Version	XM_005629113.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629113.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GTGGCGGCCA 
GAGGAGTCCG AGGACTATGA AACCATAATC AGCGGCCTGA CCCCAGAGAC CACCTATTCC 
ATTACCGTCG CCGCCTACAC CACCAAAGGG GATGGTGCCC GCAGCAAGCC CAAAGTTGTC 
ACAACGACCG GGGCAGTCCC AGGCCGGCCC ACCATGATGG TCAGCACCAC GGCCATGAAC 
ACCGCACTGC TGCAGTGGCA CCCCCCCAAG GAGCTGCCAG GGGAGCTGCT GGGCTACCGG 
CTACAGTACC ACCGTGCCGA TGAGGCAAAG CCCAACATCC TAGATTTTGG CAAGGACGAC 
CAGCACTTTA CAGTCACCGG CCTACACAAG GGAGCCACCT ACATTTTCCG GCTTGCCGCC 
AAGAACCGGG CTGGCCCGGG AGAAGAGTTC GAGAAGGAGA TCACGACTCC CGAGGATGCG 
CCCAGCGGCT TCCCCCAAAA CCTGCGCGTG ATAGGGCTGA CCACCTCTAC CACGGAGCTG 
ACCTGGGACC CCCCGGTGCT GGCGGAGAGG AACGGGCGCA TCACCAACTA CTCCGTGGTG 
TACCGTGACA TCAACAGCCA GCAGGAGCTA CAGAACGTCA CCGCCGACAC CCACCTCATG 
CTCTCCGGCC TCAAGCCGGA CACCACTTAC GACATCAAGG TCCGCGCACG TACCAGCAAG 
GGCGCCGGCC CACTCAGCCC CAGCATCCAG TCCCGGACCA TGCCCATGGA GCAAGTGTTT 
GCCAAGAACT TCCGTGTGGC GGCCGCTATG AAGACGTCTG TGCTGCTCAG CTGGGAGGTC 
CCAGACTCCT ACAAGTCGGC TGTACCCTTC AAGATCCTGT ACAACGGGCA GAGTGTGGAG 
GTGGACGGGC ACTCCATGCG GAAACTGATC GCAGACCTGC AGCCGAACAC GGAGTACTCG 
TTCGTGCTGA TGAACCGTGG CAGCAGCGCG GGGGGCCTGC AGCACCTCGT GTCCATCCGC 
ACGGCCCCAG ACCTCCTGCC ACACAAGCCG ATGCCCGCCT CCGCCTTCAT AGAGGATGGT 
CGCTTCACCC TCTCCATGCC CCACGTGCAG GATGCCTCAC TCATCAGGTG GTTCTACATC 
GTGGTGGTAC CCATCGACCG CGTGGGTGGG AGTGTGCTGG CCCCGAGATG GAGCACGCCT 
GAGGAGCTGG AGCTGGACGA GCTTCTGGAG GCCATCGAGC AGGGCGGTGG GGAGCGGCGA 
CGACGGCGGC AGGCGGAGCG GCTGAAGCCA TATGTGGCTG CACAGGTGGA CGTGCTCCCC 
GAGAGCTTCA CCCTAGGAGA CAAGAAGAGC TACCGGGGCT TCTACAACCG GCCTCTGTCT 
CCGGACCTGA GCTATCAGTG CTTTGTGCTC GCCTCCCTGA AGGAACCCGT GGACCAGAAG 
CGCTATGCCT CCAGCCCCTA CTCTGATGAG ATCGTGGTCC AAGTGACACC AGCCCAGCAG 
CAGGAGGAGC CTGAGATGCT GTGGGTGACA GGCCCCGTCC TCGCAGTCAT CCTCATCATC 
CTCATTGTCA TCGCCATCCT CCTGTTCAAG AGGAAGAGGA CTCACTCCCC ATCATCCAAG 
GACGAGCAGT CAATCGGGCT GAAGGACTCC TTGCTGGCCC ATTCCTCTGA CCCTGTGGAG 
ATGCGGAGGC TCAACTACCA GACTCCAGGT ATGCGAGACC ACCCGCCCAT CCCCATCACT 
GACCTGGCCG ACAACATTGA GCGCCTCAAA GCCAACGACG GCCTCAAGTT CTCCCAGGAG 
TACGAGTCCA TTGACCCTGG ACAGCAGTTC ACCTGGGAGA ATTCAAACCT GGAAGTGAAC 
AAGCCCAAGA ACCGCTATGC AAACGTCATC GCCTACGACC ACTCCCGAGT CATCCTTACC 
TCCGTCGATG GTGTCCCAGG GAGTGACTAT ATCAACGCCA ACTACATCGA TGGCTACCGC 
AAGCAGAACG CCTACATTGC CACGCAGGGC CCACTGCCCG AGACCATGGG CGATTTCTGG 
CGGATGGTGT GGGAACAGCG CACAGCCACC GTGGTCATGA TGACACGGCT GGAGGAGAAG 
TCTCGGGTGA AGTGTGATCA GTACTGGCCA GCCCGGGGTA CCGAGACCCA CGGCCTCATT 
CAGGTGACCC TGCTGGACAC GGTGGAGCTG GCCACATACA CTGTGCGCAC CTTTGCACTC 
CACAAGAGTG GCTCCAGCGA GAAGCGGGAG CTGCGTCAGT TCCAGTTCAT GGCCTGGCCG 
GACCACGGGG TTCCCGAGTA CCCCACCCCC ATCCTGGCCT TCCTGCGGCG GGTCAAGGCC 
TGTAACCCGC TAGACGCAGG GCCCATGGTG GTGCACTGCA GCGCGGGCGT GGGCCGCACG 
GGCTGTTTCA TCGTCATCGA CGCCATGCTG GAGCGGATGA AGCACGAGAA GACAGTGGAC 
ATCTATGGCC ACGTGACATG CATGCGATCG CAGCGGAACT ACATGGTGCA GACAGAGGAC 
CAGTACGTGT TCATCCACGA GGCGCTGCTA GAGGCCGCCA CGTGCGGACA CACGGAGGTG 
CCTGCCCGCA GCCTGTATGC CCATATCCAG AAGCTGGGCC AAGTGCCTCC CGGGGAGAGT 
GTGACTGCCA TGGAGCTCGA GTTCAAGCTG CTGGCCAGCT CCAAGGCCCA CACATCCCGC 
TTCATTAGCG CCAACCTGCC CTGCAACAAG TTCAAGAACC GCCTGGTGAA CATCATGCCC 
TATGAGTTGA CCCGTGTGTG TCTGCAGCCC ATCCGTGGTG TGGAGGGCTC TGACTACATC 
AATGCCAGCT TCTTGGACGG CTATAGGCAG CAGAAGGCCT ACATCGCCAC GCAGGGGCCC 
CTGGCAGAGA GCACCGAGGA CTTCTGGCGC ATGCTCTGGG AGCACGGCTC CACCATCGTC 
GTCATGCTGA CCAGGCTTCG GGAGATGGGC AGGGAGAAAT GCCACCAGTA CTGGCCAGCA 
GAGCGCTCCG CTCGCTACCA ATACTTTGTC GTTGACCCGA TGGCTGAGTA CAACATGCCG 
CAGTACATCC TGCGTGAGTT CAAGGTCACA GATGCCCGGG ATGGGCAGTC GAGGACAATC 
CGGCAGTTCC AGTTCACAGA CTGGCCCGAG CAGGGCGTGC CTAAGACGGG TGAGGGCTTC 
ATCGACTTCA TCGGGCAGGT GCACAAGACC AAGGAGCAGT TCGGGCAGGA CGGGCCCATC 
ACGGTGCACT GCAGTGCCGG CGTGGGCCGC ACCGGGGTGT TCATCACTCT GAGCATCGTC 
CTGGAGCGGA TGCGCTACGA GGGCGTGGTC GACATGTTCC AGACCGTGAA GACCCTGCGC 
ACACAGCGCC CAGCCATGGT GCAGACGGAG GACCAGTACC AGCTGTGCTA TCGTGCGGCC 
CTGGAGTACC TTGGCAGCTT TGACCACTAT GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629170.1
CDS	125..5863
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGGSPIRGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPAASRNGVITQYSVAYEAVDGEDRGRHVVESISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQWRPEESEDYETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_005629113.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629113.3

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GTGGCGGCCA 
GAGGAGTCCG AGGACTATGA AACCATAATC AGCGGCCTGA CCCCAGAGAC CACCTATTCC 
ATTACCGTCG CCGCCTACAC CACCAAAGGG GATGGTGCCC GCAGCAAGCC CAAAGTTGTC 
ACAACGACCG GGGCAGTCCC AGGCCGGCCC ACCATGATGG TCAGCACCAC GGCCATGAAC 
ACCGCACTGC TGCAGTGGCA CCCCCCCAAG GAGCTGCCAG GGGAGCTGCT GGGCTACCGG 
CTACAGTACC ACCGTGCCGA TGAGGCAAAG CCCAACATCC TAGATTTTGG CAAGGACGAC 
CAGCACTTTA CAGTCACCGG CCTACACAAG GGAGCCACCT ACATTTTCCG GCTTGCCGCC 
AAGAACCGGG CTGGCCCGGG AGAAGAGTTC GAGAAGGAGA TCACGACTCC CGAGGATGCG 
CCCAGCGGCT TCCCCCAAAA CCTGCGCGTG ATAGGGCTGA CCACCTCTAC CACGGAGCTG 
ACCTGGGACC CCCCGGTGCT GGCGGAGAGG AACGGGCGCA TCACCAACTA CTCCGTGGTG 
TACCGTGACA TCAACAGCCA GCAGGAGCTA CAGAACGTCA CCGCCGACAC CCACCTCATG 
CTCTCCGGCC TCAAGCCGGA CACCACTTAC GACATCAAGG TCCGCGCACG TACCAGCAAG 
GGCGCCGGCC CACTCAGCCC CAGCATCCAG TCCCGGACCA TGCCCATGGA GCAAGTGTTT 
GCCAAGAACT TCCGTGTGGC GGCCGCTATG AAGACGTCTG TGCTGCTCAG CTGGGAGGTC 
CCAGACTCCT ACAAGTCGGC TGTACCCTTC AAGATCCTGT ACAACGGGCA GAGTGTGGAG 
GTGGACGGGC ACTCCATGCG GAAACTGATC GCAGACCTGC AGCCGAACAC GGAGTACTCG 
TTCGTGCTGA TGAACCGTGG CAGCAGCGCG GGGGGCCTGC AGCACCTCGT GTCCATCCGC 
ACGGCCCCAG ACCTCCTGCC ACACAAGCCG ATGCCCGCCT CCGCCTTCAT AGAGGATGGT 
CGCTTCACCC TCTCCATGCC CCACGTGCAG GATGCCTCAC TCATCAGGTG GTTCTACATC 
GTGGTGGTAC CCATCGACCG CGTGGGTGGG AGTGTGCTGG CCCCGAGATG GAGCACGCCT 
GAGGAGCTGG AGCTGGACGA GCTTCTGGAG GCCATCGAGC AGGGCGGTGG GGAGCGGCGA 
CGACGGCGGC AGGCGGAGCG GCTGAAGCCA TATGTGGCTG CACAGGTGGA CGTGCTCCCC 
GAGAGCTTCA CCCTAGGAGA CAAGAAGAGC TACCGGGGCT TCTACAACCG GCCTCTGTCT 
CCGGACCTGA GCTATCAGTG CTTTGTGCTC GCCTCCCTGA AGGAACCCGT GGACCAGAAG 
CGCTATGCCT CCAGCCCCTA CTCTGATGAG ATCGTGGTCC AAGTGACACC AGCCCAGCAG 
CAGGAGGAGC CTGAGATGCT GTGGGTGACA GGCCCCGTCC TCGCAGTCAT CCTCATCATC 
CTCATTGTCA TCGCCATCCT CCTGTTCAAG AGGAAGAGGA CTCACTCCCC ATCATCCAAG 
GACGAGCAGT CAATCGGGCT GAAGGACTCC TTGCTGGCCC ATTCCTCTGA CCCTGTGGAG 
ATGCGGAGGC TCAACTACCA GACTCCAGGT ATGCGAGACC ACCCGCCCAT CCCCATCACT 
GACCTGGCCG ACAACATTGA GCGCCTCAAA GCCAACGACG GCCTCAAGTT CTCCCAGGAG 
TACGAGTCCA TTGACCCTGG ACAGCAGTTC ACCTGGGAGA ATTCAAACCT GGAAGTGAAC 
AAGCCCAAGA ACCGCTATGC AAACGTCATC GCCTACGACC ACTCCCGAGT CATCCTTACC 
TCCGTCGATG GTGTCCCAGG GAGTGACTAT ATCAACGCCA ACTACATCGA TGGCTACCGC 
AAGCAGAACG CCTACATTGC CACGCAGGGC CCACTGCCCG AGACCATGGG CGATTTCTGG 
CGGATGGTGT GGGAACAGCG CACAGCCACC GTGGTCATGA TGACACGGCT GGAGGAGAAG 
TCTCGGGTGA AGTGTGATCA GTACTGGCCA GCCCGGGGTA CCGAGACCCA CGGCCTCATT 
CAGGTGACCC TGCTGGACAC GGTGGAGCTG GCCACATACA CTGTGCGCAC CTTTGCACTC 
CACAAGAGTG GCTCCAGCGA GAAGCGGGAG CTGCGTCAGT TCCAGTTCAT GGCCTGGCCG 
GACCACGGGG TTCCCGAGTA CCCCACCCCC ATCCTGGCCT TCCTGCGGCG GGTCAAGGCC 
TGTAACCCGC TAGACGCAGG GCCCATGGTG GTGCACTGCA GCGCGGGCGT GGGCCGCACG 
GGCTGTTTCA TCGTCATCGA CGCCATGCTG GAGCGGATGA AGCACGAGAA GACAGTGGAC 
ATCTATGGCC ACGTGACATG CATGCGATCG CAGCGGAACT ACATGGTGCA GACAGAGGAC 
CAGTACGTGT TCATCCACGA GGCGCTGCTA GAGGCCGCCA CGTGCGGACA CACGGAGGTG 
CCTGCCCGCA GCCTGTATGC CCATATCCAG AAGCTGGGCC AAGTGCCTCC CGGGGAGAGT 
GTGACTGCCA TGGAGCTCGA GTTCAAGCTG CTGGCCAGCT CCAAGGCCCA CACATCCCGC 
TTCATTAGCG CCAACCTGCC CTGCAACAAG TTCAAGAACC GCCTGGTGAA CATCATGCCC 
TATGAGTTGA CCCGTGTGTG TCTGCAGCCC ATCCGTGGTG TGGAGGGCTC TGACTACATC 
AATGCCAGCT TCTTGGACGG CTATAGGCAG CAGAAGGCCT ACATCGCCAC GCAGGGGCCC 
CTGGCAGAGA GCACCGAGGA CTTCTGGCGC ATGCTCTGGG AGCACGGCTC CACCATCGTC 
GTCATGCTGA CCAGGCTTCG GGAGATGGGC AGGGAGAAAT GCCACCAGTA CTGGCCAGCA 
GAGCGCTCCG CTCGCTACCA ATACTTTGTC GTTGACCCGA TGGCTGAGTA CAACATGCCG 
CAGTACATCC TGCGTGAGTT CAAGGTCACA GATGCCCGGG ATGGGCAGTC GAGGACAATC 
CGGCAGTTCC AGTTCACAGA CTGGCCCGAG CAGGGCGTGC CTAAGACGGG TGAGGGCTTC 
ATCGACTTCA TCGGGCAGGT GCACAAGACC AAGGAGCAGT TCGGGCAGGA CGGGCCCATC 
ACGGTGCACT GCAGTGCCGG CGTGGGCCGC ACCGGGGTGT TCATCACTCT GAGCATCGTC 
CTGGAGCGGA TGCGCTACGA GGGCGTGGTC GACATGTTCC AGACCGTGAA GACCCTGCGC 
ACACAGCGCC CAGCCATGGT GCAGACGGAG GACCAGTACC AGCTGTGCTA TCGTGCGGCC 
CTGGAGTACC TTGGCAGCTT TGACCACTAT GCAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_532613.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_532613.2 Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X5, mRNA.

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CloneID	OCe17423
Clone ID Related Accession (Same CDS sequence)	XM_532613.6 , XM_532613.5
Accession Version	XM_532613.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_532613.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCACCCCCTC AGCCCCTCCC CAGAAGGTGA CCTGTGTGAG CACGGGCTCC 
ACCACGGTTC GGGTAAGTTG GGTCCCGCCG CCAGCTGCCA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCAGTGGAC GGCGAGGACC GAGGGCGGCA TGTGGTGGAG 
AGCATCAGTC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACTGACGTG GGCCCCGGCC CAGAGAGCAG CCCGGTGCTC 
GTGCGCACAG ACGAGGACGT GCCCAGTGGA CCGCCACGGA AGGTGGAGGT GGAACCACTG 
AATTCCACTG CTGTGCGTGT CTCTTGGAAG CTGCCCGTCC CCAGCAAGCA GCACGGCCAG 
ATTCGCGGCT ACCAGGTCAC CTATGTGCGG CTAGAGAATG GCGAGCCCCG TGGCCCACCC 
ATCATCCAGG ACGTCATGCT GGCTGAGGCC CAGTGGCGGC CAGAGGAGTC CGAGGACTAT 
GAAACCATAA TCAGCGGCCT GACCCCAGAG ACCACCTATT CCATTACCGT CGCCGCCTAC 
ACCACCAAAG GGGATGGTGC CCGCAGCAAG CCCAAAGTTG TCACAACGAC CGGGGCAGTC 
CCAGGCCGGC CCACCATGAT GGTCAGCACC ACGGCCATGA ACACCGCACT GCTGCAGTGG 
CACCCCCCCA AGGAGCTGCC AGGGGAGCTG CTGGGCTACC GGCTACAGTA CCACCGTGCC 
GATGAGGCAA AGCCCAACAT CCTAGATTTT GGCAAGGACG ACCAGCACTT TACAGTCACC 
GGCCTACACA AGGGAGCCAC CTACATTTTC CGGCTTGCCG CCAAGAACCG GGCTGGCCCG 
GGAGAAGAGT TCGAGAAGGA GATCACGACT CCCGAGGATG CGCCCAGCGG CTTCCCCCAA 
AACCTGCGCG TGATAGGGCT GACCACCTCT ACCACGGAGC TGACCTGGGA CCCCCCGGTG 
CTGGCGGAGA GGAACGGGCG CATCACCAAC TACTCCGTGG TGTACCGTGA CATCAACAGC 
CAGCAGGAGC TACAGAACGT CACCGCCGAC ACCCACCTCA TGCTCTCCGG CCTCAAGCCG 
GACACCACTT ACGACATCAA GGTCCGCGCA CGTACCAGCA AGGGCGCCGG CCCACTCAGC 
CCCAGCATCC AGTCCCGGAC CATGCCCATG GAGCAAGTGT TTGCCAAGAA CTTCCGTGTG 
GCGGCCGCTA TGAAGACGTC TGTGCTGCTC AGCTGGGAGG TCCCAGACTC CTACAAGTCG 
GCTGTACCCT TCAAGATCCT GTACAACGGG CAGAGTGTGG AGGTGGACGG GCACTCCATG 
CGGAAACTGA TCGCAGACCT GCAGCCGAAC ACGGAGTACT CGTTCGTGCT GATGAACCGT 
GGCAGCAGCG CGGGGGGCCT GCAGCACCTC GTGTCCATCC GCACGGCCCC AGACCTCCTG 
CCACACAAGC CGATGCCCGC CTCCGCCTTC ATAGAGGATG GTCGCTTCAC CCTCTCCATG 
CCCCACGTGC AGGATGCCTC ACTCATCAGG TGGTTCTACA TCGTGGTGGT ACCCATCGAC 
CGCGTGGGTG GGAGTGTGCT GGCCCCGAGA TGGAGCACGC CTGAGGAGCT GGAGCTGGAC 
GAGCTTCTGG AGGCCATCGA GCAGGGCGGT GGGGAGCGGC GACGACGGCG GCAGGCGGAG 
CGGCTGAAGC CATATGTGGC TGCACAGGTG GACGTGCTCC CCGAGAGCTT CACCCTAGGA 
GACAAGAAGA GCTACCGGGG CTTCTACAAC CGGCCTCTGT CTCCGGACCT GAGCTATCAG 
TGCTTTGTGC TCGCCTCCCT GAAGGAACCC GTGGACCAGA AGCGCTATGC CTCCAGCCCC 
TACTCTGATG AGATCGTGGT CCAAGTGACA CCAGCCCAGC AGCAGGAGGA GCCTGAGATG 
CTGTGGGTGA CAGGCCCCGT CCTCGCAGTC ATCCTCATCA TCCTCATTGT CATCGCCATC 
CTCCTGTTCA AGAGGAAGAG GACTCACTCC CCATCATCCA AGGACGAGCA GTCAATCGGG 
CTGAAGGACT CCTTGCTGGC CCATTCCTCT GACCCTGTGG AGATGCGGAG GCTCAACTAC 
CAGACTCCAG GTATGCGAGA CCACCCGCCC ATCCCCATCA CTGACCTGGC CGACAACATT 
GAGCGCCTCA AAGCCAACGA CGGCCTCAAG TTCTCCCAGG AGTACGAGTC CATTGACCCT 
GGACAGCAGT TCACCTGGGA GAATTCAAAC CTGGAAGTGA ACAAGCCCAA GAACCGCTAT 
GCAAACGTCA TCGCCTACGA CCACTCCCGA GTCATCCTTA CCTCCGTCGA TGGTGTCCCA 
GGGAGTGACT ATATCAACGC CAACTACATC GATGGCTACC GCAAGCAGAA CGCCTACATT 
GCCACGCAGG GCCCACTGCC CGAGACCATG GGCGATTTCT GGCGGATGGT GTGGGAACAG 
CGCACAGCCA CCGTGGTCAT GATGACACGG CTGGAGGAGA AGTCTCGGGT GAAGTGTGAT 
CAGTACTGGC CAGCCCGGGG TACCGAGACC CACGGCCTCA TTCAGGTGAC CCTGCTGGAC 
ACGGTGGAGC TGGCCACATA CACTGTGCGC ACCTTTGCAC TCCACAAGAG TGGCTCCAGC 
GAGAAGCGGG AGCTGCGTCA GTTCCAGTTC ATGGCCTGGC CGGACCACGG GGTTCCCGAG 
TACCCCACCC CCATCCTGGC CTTCCTGCGG CGGGTCAAGG CCTGTAACCC GCTAGACGCA 
GGGCCCATGG TGGTGCACTG CAGCGCGGGC GTGGGCCGCA CGGGCTGTTT CATCGTCATC 
GACGCCATGC TGGAGCGGAT GAAGCACGAG AAGACAGTGG ACATCTATGG CCACGTGACA 
TGCATGCGAT CGCAGCGGAA CTACATGGTG CAGACAGAGG ACCAGTACGT GTTCATCCAC 
GAGGCGCTGC TAGAGGCCGC CACGTGCGGA CACACGGAGG TGCCTGCCCG CAGCCTGTAT 
GCCCATATCC AGAAGCTGGG CCAAGTGCCT CCCGGGGAGA GTGTGACTGC CATGGAGCTC 
GAGTTCAAGC TGCTGGCCAG CTCCAAGGCC CACACATCCC GCTTCATTAG CGCCAACCTG 
CCCTGCAACA AGTTCAAGAA CCGCCTGGTG AACATCATGC CCTATGAGTT GACCCGTGTG 
TGTCTGCAGC CCATCCGTGG TGTGGAGGGC TCTGACTACA TCAATGCCAG CTTCTTGGAC 
GGCTATAGGC AGCAGAAGGC CTACATCGCC ACGCAGGGGC CCCTGGCAGA GAGCACCGAG 
GACTTCTGGC GCATGCTCTG GGAGCACGGC TCCACCATCG TCGTCATGCT GACCAGGCTT 
CGGGAGATGG GCAGGGAGAA ATGCCACCAG TACTGGCCAG CAGAGCGCTC CGCTCGCTAC 
CAATACTTTG TCGTTGACCC GATGGCTGAG TACAACATGC CGCAGTACAT CCTGCGTGAG 
TTCAAGGTCA CAGATGCCCG GGATGGGCAG TCGAGGACAA TCCGGCAGTT CCAGTTCACA 
GACTGGCCCG AGCAGGGCGT GCCTAAGACG GGTGAGGGCT TCATCGACTT CATCGGGCAG 
GTGCACAAGA CCAAGGAGCA GTTCGGGCAG GACGGGCCCA TCACGGTGCA CTGCAGTGCC 
GGCGTGGGCC GCACCGGGGT GTTCATCACT CTGAGCATCG TCCTGGAGCG GATGCGCTAC 
GAGGGCGTGG TCGACATGTT CCAGACCGTG AAGACCCTGC GCACACAGCG CCCAGCCATG 
GTGCAGACGG AGGACCAGTA CCAGCTGTGC TATCGTGCGG CCCTGGAGTA CCTTGGCAGC 
TTTGACCACT ATGCAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_532613.2
CDS	125..5845
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPAASRNGVITQYSVAYEAVDGEDRGRHVVESISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQWRPEESEDYETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_532613.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_532613.6

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCACCCCCTC AGCCCCTCCC CAGAAGGTGA CCTGTGTGAG CACGGGCTCC 
ACCACGGTTC GGGTAAGTTG GGTCCCGCCG CCAGCTGCCA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCAGTGGAC GGCGAGGACC GAGGGCGGCA TGTGGTGGAG 
AGCATCAGTC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACTGACGTG GGCCCCGGCC CAGAGAGCAG CCCGGTGCTC 
GTGCGCACAG ACGAGGACGT GCCCAGTGGA CCGCCACGGA AGGTGGAGGT GGAACCACTG 
AATTCCACTG CTGTGCGTGT CTCTTGGAAG CTGCCCGTCC CCAGCAAGCA GCACGGCCAG 
ATTCGCGGCT ACCAGGTCAC CTATGTGCGG CTAGAGAATG GCGAGCCCCG TGGCCCACCC 
ATCATCCAGG ACGTCATGCT GGCTGAGGCC CAGTGGCGGC CAGAGGAGTC CGAGGACTAT 
GAAACCATAA TCAGCGGCCT GACCCCAGAG ACCACCTATT CCATTACCGT CGCCGCCTAC 
ACCACCAAAG GGGATGGTGC CCGCAGCAAG CCCAAAGTTG TCACAACGAC CGGGGCAGTC 
CCAGGCCGGC CCACCATGAT GGTCAGCACC ACGGCCATGA ACACCGCACT GCTGCAGTGG 
CACCCCCCCA AGGAGCTGCC AGGGGAGCTG CTGGGCTACC GGCTACAGTA CCACCGTGCC 
GATGAGGCAA AGCCCAACAT CCTAGATTTT GGCAAGGACG ACCAGCACTT TACAGTCACC 
GGCCTACACA AGGGAGCCAC CTACATTTTC CGGCTTGCCG CCAAGAACCG GGCTGGCCCG 
GGAGAAGAGT TCGAGAAGGA GATCACGACT CCCGAGGATG CGCCCAGCGG CTTCCCCCAA 
AACCTGCGCG TGATAGGGCT GACCACCTCT ACCACGGAGC TGACCTGGGA CCCCCCGGTG 
CTGGCGGAGA GGAACGGGCG CATCACCAAC TACTCCGTGG TGTACCGTGA CATCAACAGC 
CAGCAGGAGC TACAGAACGT CACCGCCGAC ACCCACCTCA TGCTCTCCGG CCTCAAGCCG 
GACACCACTT ACGACATCAA GGTCCGCGCA CGTACCAGCA AGGGCGCCGG CCCACTCAGC 
CCCAGCATCC AGTCCCGGAC CATGCCCATG GAGCAAGTGT TTGCCAAGAA CTTCCGTGTG 
GCGGCCGCTA TGAAGACGTC TGTGCTGCTC AGCTGGGAGG TCCCAGACTC CTACAAGTCG 
GCTGTACCCT TCAAGATCCT GTACAACGGG CAGAGTGTGG AGGTGGACGG GCACTCCATG 
CGGAAACTGA TCGCAGACCT GCAGCCGAAC ACGGAGTACT CGTTCGTGCT GATGAACCGT 
GGCAGCAGCG CGGGGGGCCT GCAGCACCTC GTGTCCATCC GCACGGCCCC AGACCTCCTG 
CCACACAAGC CGATGCCCGC CTCCGCCTTC ATAGAGGATG GTCGCTTCAC CCTCTCCATG 
CCCCACGTGC AGGATGCCTC ACTCATCAGG TGGTTCTACA TCGTGGTGGT ACCCATCGAC 
CGCGTGGGTG GGAGTGTGCT GGCCCCGAGA TGGAGCACGC CTGAGGAGCT GGAGCTGGAC 
GAGCTTCTGG AGGCCATCGA GCAGGGCGGT GGGGAGCGGC GACGACGGCG GCAGGCGGAG 
CGGCTGAAGC CATATGTGGC TGCACAGGTG GACGTGCTCC CCGAGAGCTT CACCCTAGGA 
GACAAGAAGA GCTACCGGGG CTTCTACAAC CGGCCTCTGT CTCCGGACCT GAGCTATCAG 
TGCTTTGTGC TCGCCTCCCT GAAGGAACCC GTGGACCAGA AGCGCTATGC CTCCAGCCCC 
TACTCTGATG AGATCGTGGT CCAAGTGACA CCAGCCCAGC AGCAGGAGGA GCCTGAGATG 
CTGTGGGTGA CAGGCCCCGT CCTCGCAGTC ATCCTCATCA TCCTCATTGT CATCGCCATC 
CTCCTGTTCA AGAGGAAGAG GACTCACTCC CCATCATCCA AGGACGAGCA GTCAATCGGG 
CTGAAGGACT CCTTGCTGGC CCATTCCTCT GACCCTGTGG AGATGCGGAG GCTCAACTAC 
CAGACTCCAG GTATGCGAGA CCACCCGCCC ATCCCCATCA CTGACCTGGC CGACAACATT 
GAGCGCCTCA AAGCCAACGA CGGCCTCAAG TTCTCCCAGG AGTACGAGTC CATTGACCCT 
GGACAGCAGT TCACCTGGGA GAATTCAAAC CTGGAAGTGA ACAAGCCCAA GAACCGCTAT 
GCAAACGTCA TCGCCTACGA CCACTCCCGA GTCATCCTTA CCTCCGTCGA TGGTGTCCCA 
GGGAGTGACT ATATCAACGC CAACTACATC GATGGCTACC GCAAGCAGAA CGCCTACATT 
GCCACGCAGG GCCCACTGCC CGAGACCATG GGCGATTTCT GGCGGATGGT GTGGGAACAG 
CGCACAGCCA CCGTGGTCAT GATGACACGG CTGGAGGAGA AGTCTCGGGT GAAGTGTGAT 
CAGTACTGGC CAGCCCGGGG TACCGAGACC CACGGCCTCA TTCAGGTGAC CCTGCTGGAC 
ACGGTGGAGC TGGCCACATA CACTGTGCGC ACCTTTGCAC TCCACAAGAG TGGCTCCAGC 
GAGAAGCGGG AGCTGCGTCA GTTCCAGTTC ATGGCCTGGC CGGACCACGG GGTTCCCGAG 
TACCCCACCC CCATCCTGGC CTTCCTGCGG CGGGTCAAGG CCTGTAACCC GCTAGACGCA 
GGGCCCATGG TGGTGCACTG CAGCGCGGGC GTGGGCCGCA CGGGCTGTTT CATCGTCATC 
GACGCCATGC TGGAGCGGAT GAAGCACGAG AAGACAGTGG ACATCTATGG CCACGTGACA 
TGCATGCGAT CGCAGCGGAA CTACATGGTG CAGACAGAGG ACCAGTACGT GTTCATCCAC 
GAGGCGCTGC TAGAGGCCGC CACGTGCGGA CACACGGAGG TGCCTGCCCG CAGCCTGTAT 
GCCCATATCC AGAAGCTGGG CCAAGTGCCT CCCGGGGAGA GTGTGACTGC CATGGAGCTC 
GAGTTCAAGC TGCTGGCCAG CTCCAAGGCC CACACATCCC GCTTCATTAG CGCCAACCTG 
CCCTGCAACA AGTTCAAGAA CCGCCTGGTG AACATCATGC CCTATGAGTT GACCCGTGTG 
TGTCTGCAGC CCATCCGTGG TGTGGAGGGC TCTGACTACA TCAATGCCAG CTTCTTGGAC 
GGCTATAGGC AGCAGAAGGC CTACATCGCC ACGCAGGGGC CCCTGGCAGA GAGCACCGAG 
GACTTCTGGC GCATGCTCTG GGAGCACGGC TCCACCATCG TCGTCATGCT GACCAGGCTT 
CGGGAGATGG GCAGGGAGAA ATGCCACCAG TACTGGCCAG CAGAGCGCTC CGCTCGCTAC 
CAATACTTTG TCGTTGACCC GATGGCTGAG TACAACATGC CGCAGTACAT CCTGCGTGAG 
TTCAAGGTCA CAGATGCCCG GGATGGGCAG TCGAGGACAA TCCGGCAGTT CCAGTTCACA 
GACTGGCCCG AGCAGGGCGT GCCTAAGACG GGTGAGGGCT TCATCGACTT CATCGGGCAG 
GTGCACAAGA CCAAGGAGCA GTTCGGGCAG GACGGGCCCA TCACGGTGCA CTGCAGTGCC 
GGCGTGGGCC GCACCGGGGT GTTCATCACT CTGAGCATCG TCCTGGAGCG GATGCGCTAC 
GAGGGCGTGG TCGACATGTT CCAGACCGTG AAGACCCTGC GCACACAGCG CCCAGCCATG 
GTGCAGACGG AGGACCAGTA CCAGCTGTGC TATCGTGCGG CCCTGGAGTA CCTTGGCAGC 
TTTGACCACT ATGCAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_532613.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_532613.2 Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X5, mRNA.

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CloneID	OCe17423
Clone ID Related Accession (Same CDS sequence)	XM_532613.6 , XM_532613.5
Accession Version	XM_532613.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_532613.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCACCCCCTC AGCCCCTCCC CAGAAGGTGA CCTGTGTGAG CACGGGCTCC 
ACCACGGTTC GGGTAAGTTG GGTCCCGCCG CCAGCTGCCA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCAGTGGAC GGCGAGGACC GAGGGCGGCA TGTGGTGGAG 
AGCATCAGTC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACTGACGTG GGCCCCGGCC CAGAGAGCAG CCCGGTGCTC 
GTGCGCACAG ACGAGGACGT GCCCAGTGGA CCGCCACGGA AGGTGGAGGT GGAACCACTG 
AATTCCACTG CTGTGCGTGT CTCTTGGAAG CTGCCCGTCC CCAGCAAGCA GCACGGCCAG 
ATTCGCGGCT ACCAGGTCAC CTATGTGCGG CTAGAGAATG GCGAGCCCCG TGGCCCACCC 
ATCATCCAGG ACGTCATGCT GGCTGAGGCC CAGTGGCGGC CAGAGGAGTC CGAGGACTAT 
GAAACCATAA TCAGCGGCCT GACCCCAGAG ACCACCTATT CCATTACCGT CGCCGCCTAC 
ACCACCAAAG GGGATGGTGC CCGCAGCAAG CCCAAAGTTG TCACAACGAC CGGGGCAGTC 
CCAGGCCGGC CCACCATGAT GGTCAGCACC ACGGCCATGA ACACCGCACT GCTGCAGTGG 
CACCCCCCCA AGGAGCTGCC AGGGGAGCTG CTGGGCTACC GGCTACAGTA CCACCGTGCC 
GATGAGGCAA AGCCCAACAT CCTAGATTTT GGCAAGGACG ACCAGCACTT TACAGTCACC 
GGCCTACACA AGGGAGCCAC CTACATTTTC CGGCTTGCCG CCAAGAACCG GGCTGGCCCG 
GGAGAAGAGT TCGAGAAGGA GATCACGACT CCCGAGGATG CGCCCAGCGG CTTCCCCCAA 
AACCTGCGCG TGATAGGGCT GACCACCTCT ACCACGGAGC TGACCTGGGA CCCCCCGGTG 
CTGGCGGAGA GGAACGGGCG CATCACCAAC TACTCCGTGG TGTACCGTGA CATCAACAGC 
CAGCAGGAGC TACAGAACGT CACCGCCGAC ACCCACCTCA TGCTCTCCGG CCTCAAGCCG 
GACACCACTT ACGACATCAA GGTCCGCGCA CGTACCAGCA AGGGCGCCGG CCCACTCAGC 
CCCAGCATCC AGTCCCGGAC CATGCCCATG GAGCAAGTGT TTGCCAAGAA CTTCCGTGTG 
GCGGCCGCTA TGAAGACGTC TGTGCTGCTC AGCTGGGAGG TCCCAGACTC CTACAAGTCG 
GCTGTACCCT TCAAGATCCT GTACAACGGG CAGAGTGTGG AGGTGGACGG GCACTCCATG 
CGGAAACTGA TCGCAGACCT GCAGCCGAAC ACGGAGTACT CGTTCGTGCT GATGAACCGT 
GGCAGCAGCG CGGGGGGCCT GCAGCACCTC GTGTCCATCC GCACGGCCCC AGACCTCCTG 
CCACACAAGC CGATGCCCGC CTCCGCCTTC ATAGAGGATG GTCGCTTCAC CCTCTCCATG 
CCCCACGTGC AGGATGCCTC ACTCATCAGG TGGTTCTACA TCGTGGTGGT ACCCATCGAC 
CGCGTGGGTG GGAGTGTGCT GGCCCCGAGA TGGAGCACGC CTGAGGAGCT GGAGCTGGAC 
GAGCTTCTGG AGGCCATCGA GCAGGGCGGT GGGGAGCGGC GACGACGGCG GCAGGCGGAG 
CGGCTGAAGC CATATGTGGC TGCACAGGTG GACGTGCTCC CCGAGAGCTT CACCCTAGGA 
GACAAGAAGA GCTACCGGGG CTTCTACAAC CGGCCTCTGT CTCCGGACCT GAGCTATCAG 
TGCTTTGTGC TCGCCTCCCT GAAGGAACCC GTGGACCAGA AGCGCTATGC CTCCAGCCCC 
TACTCTGATG AGATCGTGGT CCAAGTGACA CCAGCCCAGC AGCAGGAGGA GCCTGAGATG 
CTGTGGGTGA CAGGCCCCGT CCTCGCAGTC ATCCTCATCA TCCTCATTGT CATCGCCATC 
CTCCTGTTCA AGAGGAAGAG GACTCACTCC CCATCATCCA AGGACGAGCA GTCAATCGGG 
CTGAAGGACT CCTTGCTGGC CCATTCCTCT GACCCTGTGG AGATGCGGAG GCTCAACTAC 
CAGACTCCAG GTATGCGAGA CCACCCGCCC ATCCCCATCA CTGACCTGGC CGACAACATT 
GAGCGCCTCA AAGCCAACGA CGGCCTCAAG TTCTCCCAGG AGTACGAGTC CATTGACCCT 
GGACAGCAGT TCACCTGGGA GAATTCAAAC CTGGAAGTGA ACAAGCCCAA GAACCGCTAT 
GCAAACGTCA TCGCCTACGA CCACTCCCGA GTCATCCTTA CCTCCGTCGA TGGTGTCCCA 
GGGAGTGACT ATATCAACGC CAACTACATC GATGGCTACC GCAAGCAGAA CGCCTACATT 
GCCACGCAGG GCCCACTGCC CGAGACCATG GGCGATTTCT GGCGGATGGT GTGGGAACAG 
CGCACAGCCA CCGTGGTCAT GATGACACGG CTGGAGGAGA AGTCTCGGGT GAAGTGTGAT 
CAGTACTGGC CAGCCCGGGG TACCGAGACC CACGGCCTCA TTCAGGTGAC CCTGCTGGAC 
ACGGTGGAGC TGGCCACATA CACTGTGCGC ACCTTTGCAC TCCACAAGAG TGGCTCCAGC 
GAGAAGCGGG AGCTGCGTCA GTTCCAGTTC ATGGCCTGGC CGGACCACGG GGTTCCCGAG 
TACCCCACCC CCATCCTGGC CTTCCTGCGG CGGGTCAAGG CCTGTAACCC GCTAGACGCA 
GGGCCCATGG TGGTGCACTG CAGCGCGGGC GTGGGCCGCA CGGGCTGTTT CATCGTCATC 
GACGCCATGC TGGAGCGGAT GAAGCACGAG AAGACAGTGG ACATCTATGG CCACGTGACA 
TGCATGCGAT CGCAGCGGAA CTACATGGTG CAGACAGAGG ACCAGTACGT GTTCATCCAC 
GAGGCGCTGC TAGAGGCCGC CACGTGCGGA CACACGGAGG TGCCTGCCCG CAGCCTGTAT 
GCCCATATCC AGAAGCTGGG CCAAGTGCCT CCCGGGGAGA GTGTGACTGC CATGGAGCTC 
GAGTTCAAGC TGCTGGCCAG CTCCAAGGCC CACACATCCC GCTTCATTAG CGCCAACCTG 
CCCTGCAACA AGTTCAAGAA CCGCCTGGTG AACATCATGC CCTATGAGTT GACCCGTGTG 
TGTCTGCAGC CCATCCGTGG TGTGGAGGGC TCTGACTACA TCAATGCCAG CTTCTTGGAC 
GGCTATAGGC AGCAGAAGGC CTACATCGCC ACGCAGGGGC CCCTGGCAGA GAGCACCGAG 
GACTTCTGGC GCATGCTCTG GGAGCACGGC TCCACCATCG TCGTCATGCT GACCAGGCTT 
CGGGAGATGG GCAGGGAGAA ATGCCACCAG TACTGGCCAG CAGAGCGCTC CGCTCGCTAC 
CAATACTTTG TCGTTGACCC GATGGCTGAG TACAACATGC CGCAGTACAT CCTGCGTGAG 
TTCAAGGTCA CAGATGCCCG GGATGGGCAG TCGAGGACAA TCCGGCAGTT CCAGTTCACA 
GACTGGCCCG AGCAGGGCGT GCCTAAGACG GGTGAGGGCT TCATCGACTT CATCGGGCAG 
GTGCACAAGA CCAAGGAGCA GTTCGGGCAG GACGGGCCCA TCACGGTGCA CTGCAGTGCC 
GGCGTGGGCC GCACCGGGGT GTTCATCACT CTGAGCATCG TCCTGGAGCG GATGCGCTAC 
GAGGGCGTGG TCGACATGTT CCAGACCGTG AAGACCCTGC GCACACAGCG CCCAGCCATG 
GTGCAGACGG AGGACCAGTA CCAGCTGTGC TATCGTGCGG CCCTGGAGTA CCTTGGCAGC 
TTTGACCACT ATGCAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_532613.2
CDS	125..5845
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPAASRNGVITQYSVAYEAVDGEDRGRHVVESISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQWRPEESEDYETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_532613.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_532613.5

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCACCCCCTC AGCCCCTCCC CAGAAGGTGA CCTGTGTGAG CACGGGCTCC 
ACCACGGTTC GGGTAAGTTG GGTCCCGCCG CCAGCTGCCA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCAGTGGAC GGCGAGGACC GAGGGCGGCA TGTGGTGGAG 
AGCATCAGTC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACTGACGTG GGCCCCGGCC CAGAGAGCAG CCCGGTGCTC 
GTGCGCACAG ACGAGGACGT GCCCAGTGGA CCGCCACGGA AGGTGGAGGT GGAACCACTG 
AATTCCACTG CTGTGCGTGT CTCTTGGAAG CTGCCCGTCC CCAGCAAGCA GCACGGCCAG 
ATTCGCGGCT ACCAGGTCAC CTATGTGCGG CTAGAGAATG GCGAGCCCCG TGGCCCACCC 
ATCATCCAGG ACGTCATGCT GGCTGAGGCC CAGTGGCGGC CAGAGGAGTC CGAGGACTAT 
GAAACCATAA TCAGCGGCCT GACCCCAGAG ACCACCTATT CCATTACCGT CGCCGCCTAC 
ACCACCAAAG GGGATGGTGC CCGCAGCAAG CCCAAAGTTG TCACAACGAC CGGGGCAGTC 
CCAGGCCGGC CCACCATGAT GGTCAGCACC ACGGCCATGA ACACCGCACT GCTGCAGTGG 
CACCCCCCCA AGGAGCTGCC AGGGGAGCTG CTGGGCTACC GGCTACAGTA CCACCGTGCC 
GATGAGGCAA AGCCCAACAT CCTAGATTTT GGCAAGGACG ACCAGCACTT TACAGTCACC 
GGCCTACACA AGGGAGCCAC CTACATTTTC CGGCTTGCCG CCAAGAACCG GGCTGGCCCG 
GGAGAAGAGT TCGAGAAGGA GATCACGACT CCCGAGGATG CGCCCAGCGG CTTCCCCCAA 
AACCTGCGCG TGATAGGGCT GACCACCTCT ACCACGGAGC TGACCTGGGA CCCCCCGGTG 
CTGGCGGAGA GGAACGGGCG CATCACCAAC TACTCCGTGG TGTACCGTGA CATCAACAGC 
CAGCAGGAGC TACAGAACGT CACCGCCGAC ACCCACCTCA TGCTCTCCGG CCTCAAGCCG 
GACACCACTT ACGACATCAA GGTCCGCGCA CGTACCAGCA AGGGCGCCGG CCCACTCAGC 
CCCAGCATCC AGTCCCGGAC CATGCCCATG GAGCAAGTGT TTGCCAAGAA CTTCCGTGTG 
GCGGCCGCTA TGAAGACGTC TGTGCTGCTC AGCTGGGAGG TCCCAGACTC CTACAAGTCG 
GCTGTACCCT TCAAGATCCT GTACAACGGG CAGAGTGTGG AGGTGGACGG GCACTCCATG 
CGGAAACTGA TCGCAGACCT GCAGCCGAAC ACGGAGTACT CGTTCGTGCT GATGAACCGT 
GGCAGCAGCG CGGGGGGCCT GCAGCACCTC GTGTCCATCC GCACGGCCCC AGACCTCCTG 
CCACACAAGC CGATGCCCGC CTCCGCCTTC ATAGAGGATG GTCGCTTCAC CCTCTCCATG 
CCCCACGTGC AGGATGCCTC ACTCATCAGG TGGTTCTACA TCGTGGTGGT ACCCATCGAC 
CGCGTGGGTG GGAGTGTGCT GGCCCCGAGA TGGAGCACGC CTGAGGAGCT GGAGCTGGAC 
GAGCTTCTGG AGGCCATCGA GCAGGGCGGT GGGGAGCGGC GACGACGGCG GCAGGCGGAG 
CGGCTGAAGC CATATGTGGC TGCACAGGTG GACGTGCTCC CCGAGAGCTT CACCCTAGGA 
GACAAGAAGA GCTACCGGGG CTTCTACAAC CGGCCTCTGT CTCCGGACCT GAGCTATCAG 
TGCTTTGTGC TCGCCTCCCT GAAGGAACCC GTGGACCAGA AGCGCTATGC CTCCAGCCCC 
TACTCTGATG AGATCGTGGT CCAAGTGACA CCAGCCCAGC AGCAGGAGGA GCCTGAGATG 
CTGTGGGTGA CAGGCCCCGT CCTCGCAGTC ATCCTCATCA TCCTCATTGT CATCGCCATC 
CTCCTGTTCA AGAGGAAGAG GACTCACTCC CCATCATCCA AGGACGAGCA GTCAATCGGG 
CTGAAGGACT CCTTGCTGGC CCATTCCTCT GACCCTGTGG AGATGCGGAG GCTCAACTAC 
CAGACTCCAG GTATGCGAGA CCACCCGCCC ATCCCCATCA CTGACCTGGC CGACAACATT 
GAGCGCCTCA AAGCCAACGA CGGCCTCAAG TTCTCCCAGG AGTACGAGTC CATTGACCCT 
GGACAGCAGT TCACCTGGGA GAATTCAAAC CTGGAAGTGA ACAAGCCCAA GAACCGCTAT 
GCAAACGTCA TCGCCTACGA CCACTCCCGA GTCATCCTTA CCTCCGTCGA TGGTGTCCCA 
GGGAGTGACT ATATCAACGC CAACTACATC GATGGCTACC GCAAGCAGAA CGCCTACATT 
GCCACGCAGG GCCCACTGCC CGAGACCATG GGCGATTTCT GGCGGATGGT GTGGGAACAG 
CGCACAGCCA CCGTGGTCAT GATGACACGG CTGGAGGAGA AGTCTCGGGT GAAGTGTGAT 
CAGTACTGGC CAGCCCGGGG TACCGAGACC CACGGCCTCA TTCAGGTGAC CCTGCTGGAC 
ACGGTGGAGC TGGCCACATA CACTGTGCGC ACCTTTGCAC TCCACAAGAG TGGCTCCAGC 
GAGAAGCGGG AGCTGCGTCA GTTCCAGTTC ATGGCCTGGC CGGACCACGG GGTTCCCGAG 
TACCCCACCC CCATCCTGGC CTTCCTGCGG CGGGTCAAGG CCTGTAACCC GCTAGACGCA 
GGGCCCATGG TGGTGCACTG CAGCGCGGGC GTGGGCCGCA CGGGCTGTTT CATCGTCATC 
GACGCCATGC TGGAGCGGAT GAAGCACGAG AAGACAGTGG ACATCTATGG CCACGTGACA 
TGCATGCGAT CGCAGCGGAA CTACATGGTG CAGACAGAGG ACCAGTACGT GTTCATCCAC 
GAGGCGCTGC TAGAGGCCGC CACGTGCGGA CACACGGAGG TGCCTGCCCG CAGCCTGTAT 
GCCCATATCC AGAAGCTGGG CCAAGTGCCT CCCGGGGAGA GTGTGACTGC CATGGAGCTC 
GAGTTCAAGC TGCTGGCCAG CTCCAAGGCC CACACATCCC GCTTCATTAG CGCCAACCTG 
CCCTGCAACA AGTTCAAGAA CCGCCTGGTG AACATCATGC CCTATGAGTT GACCCGTGTG 
TGTCTGCAGC CCATCCGTGG TGTGGAGGGC TCTGACTACA TCAATGCCAG CTTCTTGGAC 
GGCTATAGGC AGCAGAAGGC CTACATCGCC ACGCAGGGGC CCCTGGCAGA GAGCACCGAG 
GACTTCTGGC GCATGCTCTG GGAGCACGGC TCCACCATCG TCGTCATGCT GACCAGGCTT 
CGGGAGATGG GCAGGGAGAA ATGCCACCAG TACTGGCCAG CAGAGCGCTC CGCTCGCTAC 
CAATACTTTG TCGTTGACCC GATGGCTGAG TACAACATGC CGCAGTACAT CCTGCGTGAG 
TTCAAGGTCA CAGATGCCCG GGATGGGCAG TCGAGGACAA TCCGGCAGTT CCAGTTCACA 
GACTGGCCCG AGCAGGGCGT GCCTAAGACG GGTGAGGGCT TCATCGACTT CATCGGGCAG 
GTGCACAAGA CCAAGGAGCA GTTCGGGCAG GACGGGCCCA TCACGGTGCA CTGCAGTGCC 
GGCGTGGGCC GCACCGGGGT GTTCATCACT CTGAGCATCG TCCTGGAGCG GATGCGCTAC 
GAGGGCGTGG TCGACATGTT CCAGACCGTG AAGACCCTGC GCACACAGCG CCCAGCCATG 
GTGCAGACGG AGGACCAGTA CCAGCTGTGC TATCGTGCGG CCCTGGAGTA CCTTGGCAGC 
TTTGACCACT ATGCAACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_857873.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_862966.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X6, mRNA.

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CloneID	OCe17425
Clone ID Related Accession (Same CDS sequence)	XM_857873.4 , XM_857873.5
Accession Version	XM_857873.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_857873.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCACCCCCTC AGCCCCTCCC CAGAAGGTGA CCTGTGTGAG CACGGGCTCC 
ACCACGGTTC GGGTAAGTTG GGTCCCGCCG CCAGCTGCCA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCAGTGGAC GGCGAGGACC GAGGGCGGCA TGTGGTGGAG 
AGCATCAGTC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACTGACGTG GGCCCCGGCC CAGAGAGCAG CCCGGTGCTC 
GTGCGCACAG ACGAGGACGT GCCCAGTGGA CCGCCACGGA AGGTGGAGGT GGAACCACTG 
AATTCCACTG CTGTGCGTGT CTCTTGGAAG CTGCCCGTCC CCAGCAAGCA GCACGGCCAG 
ATTCGCGGCT ACCAGGTCAC CTATGTGCGG CTAGAGAATG GCGAGCCCCG TGGCCCACCC 
ATCATCCAGG ACGTCATGCT GGCTGAGGCC CAGGAAACCA TAATCAGCGG CCTGACCCCA 
GAGACCACCT ATTCCATTAC CGTCGCCGCC TACACCACCA AAGGGGATGG TGCCCGCAGC 
AAGCCCAAAG TTGTCACAAC GACCGGGGCA GTCCCAGGCC GGCCCACCAT GATGGTCAGC 
ACCACGGCCA TGAACACCGC ACTGCTGCAG TGGCACCCCC CCAAGGAGCT GCCAGGGGAG 
CTGCTGGGCT ACCGGCTACA GTACCACCGT GCCGATGAGG CAAAGCCCAA CATCCTAGAT 
TTTGGCAAGG ACGACCAGCA CTTTACAGTC ACCGGCCTAC ACAAGGGAGC CACCTACATT 
TTCCGGCTTG CCGCCAAGAA CCGGGCTGGC CCGGGAGAAG AGTTCGAGAA GGAGATCACG 
ACTCCCGAGG ATGCGCCCAG CGGCTTCCCC CAAAACCTGC GCGTGATAGG GCTGACCACC 
TCTACCACGG AGCTGACCTG GGACCCCCCG GTGCTGGCGG AGAGGAACGG GCGCATCACC 
AACTACTCCG TGGTGTACCG TGACATCAAC AGCCAGCAGG AGCTACAGAA CGTCACCGCC 
GACACCCACC TCATGCTCTC CGGCCTCAAG CCGGACACCA CTTACGACAT CAAGGTCCGC 
GCACGTACCA GCAAGGGCGC CGGCCCACTC AGCCCCAGCA TCCAGTCCCG GACCATGCCC 
ATGGAGCAAG TGTTTGCCAA GAACTTCCGT GTGGCGGCCG CTATGAAGAC GTCTGTGCTG 
CTCAGCTGGG AGGTCCCAGA CTCCTACAAG TCGGCTGTAC CCTTCAAGAT CCTGTACAAC 
GGGCAGAGTG TGGAGGTGGA CGGGCACTCC ATGCGGAAAC TGATCGCAGA CCTGCAGCCG 
AACACGGAGT ACTCGTTCGT GCTGATGAAC CGTGGCAGCA GCGCGGGGGG CCTGCAGCAC 
CTCGTGTCCA TCCGCACGGC CCCAGACCTC CTGCCACACA AGCCGATGCC CGCCTCCGCC 
TTCATAGAGG ATGGTCGCTT CACCCTCTCC ATGCCCCACG TGCAGGATGC CTCACTCATC 
AGGTGGTTCT ACATCGTGGT GGTACCCATC GACCGCGTGG GTGGGAGTGT GCTGGCCCCG 
AGATGGAGCA CGCCTGAGGA GCTGGAGCTG GACGAGCTTC TGGAGGCCAT CGAGCAGGGC 
GGTGGGGAGC GGCGACGACG GCGGCAGGCG GAGCGGCTGA AGCCATATGT GGCTGCACAG 
GTGGACGTGC TCCCCGAGAG CTTCACCCTA GGAGACAAGA AGAGCTACCG GGGCTTCTAC 
AACCGGCCTC TGTCTCCGGA CCTGAGCTAT CAGTGCTTTG TGCTCGCCTC CCTGAAGGAA 
CCCGTGGACC AGAAGCGCTA TGCCTCCAGC CCCTACTCTG ATGAGATCGT GGTCCAAGTG 
ACACCAGCCC AGCAGCAGGA GGAGCCTGAG ATGCTGTGGG TGACAGGCCC CGTCCTCGCA 
GTCATCCTCA TCATCCTCAT TGTCATCGCC ATCCTCCTGT TCAAGAGGAA GAGGACTCAC 
TCCCCATCAT CCAAGGACGA GCAGTCAATC GGGCTGAAGG ACTCCTTGCT GGCCCATTCC 
TCTGACCCTG TGGAGATGCG GAGGCTCAAC TACCAGACTC CAGGTATGCG AGACCACCCG 
CCCATCCCCA TCACTGACCT GGCCGACAAC ATTGAGCGCC TCAAAGCCAA CGACGGCCTC 
AAGTTCTCCC AGGAGTACGA GTCCATTGAC CCTGGACAGC AGTTCACCTG GGAGAATTCA 
AACCTGGAAG TGAACAAGCC CAAGAACCGC TATGCAAACG TCATCGCCTA CGACCACTCC 
CGAGTCATCC TTACCTCCGT CGATGGTGTC CCAGGGAGTG ACTATATCAA CGCCAACTAC 
ATCGATGGCT ACCGCAAGCA GAACGCCTAC ATTGCCACGC AGGGCCCACT GCCCGAGACC 
ATGGGCGATT TCTGGCGGAT GGTGTGGGAA CAGCGCACAG CCACCGTGGT CATGATGACA 
CGGCTGGAGG AGAAGTCTCG GGTGAAGTGT GATCAGTACT GGCCAGCCCG GGGTACCGAG 
ACCCACGGCC TCATTCAGGT GACCCTGCTG GACACGGTGG AGCTGGCCAC ATACACTGTG 
CGCACCTTTG CACTCCACAA GAGTGGCTCC AGCGAGAAGC GGGAGCTGCG TCAGTTCCAG 
TTCATGGCCT GGCCGGACCA CGGGGTTCCC GAGTACCCCA CCCCCATCCT GGCCTTCCTG 
CGGCGGGTCA AGGCCTGTAA CCCGCTAGAC GCAGGGCCCA TGGTGGTGCA CTGCAGCGCG 
GGCGTGGGCC GCACGGGCTG TTTCATCGTC ATCGACGCCA TGCTGGAGCG GATGAAGCAC 
GAGAAGACAG TGGACATCTA TGGCCACGTG ACATGCATGC GATCGCAGCG GAACTACATG 
GTGCAGACAG AGGACCAGTA CGTGTTCATC CACGAGGCGC TGCTAGAGGC CGCCACGTGC 
GGACACACGG AGGTGCCTGC CCGCAGCCTG TATGCCCATA TCCAGAAGCT GGGCCAAGTG 
CCTCCCGGGG AGAGTGTGAC TGCCATGGAG CTCGAGTTCA AGCTGCTGGC CAGCTCCAAG 
GCCCACACAT CCCGCTTCAT TAGCGCCAAC CTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTGAACATCA TGCCCTATGA GTTGACCCGT GTGTGTCTGC AGCCCATCCG TGGTGTGGAG 
GGCTCTGACT ACATCAATGC CAGCTTCTTG GACGGCTATA GGCAGCAGAA GGCCTACATC 
GCCACGCAGG GGCCCCTGGC AGAGAGCACC GAGGACTTCT GGCGCATGCT CTGGGAGCAC 
GGCTCCACCA TCGTCGTCAT GCTGACCAGG CTTCGGGAGA TGGGCAGGGA GAAATGCCAC 
CAGTACTGGC CAGCAGAGCG CTCCGCTCGC TACCAATACT TTGTCGTTGA CCCGATGGCT 
GAGTACAACA TGCCGCAGTA CATCCTGCGT GAGTTCAAGG TCACAGATGC CCGGGATGGG 
CAGTCGAGGA CAATCCGGCA GTTCCAGTTC ACAGACTGGC CCGAGCAGGG CGTGCCTAAG 
ACGGGTGAGG GCTTCATCGA CTTCATCGGG CAGGTGCACA AGACCAAGGA GCAGTTCGGG 
CAGGACGGGC CCATCACGGT GCACTGCAGT GCCGGCGTGG GCCGCACCGG GGTGTTCATC 
ACTCTGAGCA TCGTCCTGGA GCGGATGCGC TACGAGGGCG TGGTCGACAT GTTCCAGACC 
GTGAAGACCC TGCGCACACA GCGCCCAGCC ATGGTGCAGA CGGAGGACCA GTACCAGCTG 
TGCTATCGTG CGGCCCTGGA GTACCTTGGC AGCTTTGACC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_862966.1
CDS	125..5818
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPAASRNGVITQYSVAYEAVDGEDRGRHVVESISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_857873.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_857873.4

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCACCCCCTC AGCCCCTCCC CAGAAGGTGA CCTGTGTGAG CACGGGCTCC 
ACCACGGTTC GGGTAAGTTG GGTCCCGCCG CCAGCTGCCA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCAGTGGAC GGCGAGGACC GAGGGCGGCA TGTGGTGGAG 
AGCATCAGTC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACTGACGTG GGCCCCGGCC CAGAGAGCAG CCCGGTGCTC 
GTGCGCACAG ACGAGGACGT GCCCAGTGGA CCGCCACGGA AGGTGGAGGT GGAACCACTG 
AATTCCACTG CTGTGCGTGT CTCTTGGAAG CTGCCCGTCC CCAGCAAGCA GCACGGCCAG 
ATTCGCGGCT ACCAGGTCAC CTATGTGCGG CTAGAGAATG GCGAGCCCCG TGGCCCACCC 
ATCATCCAGG ACGTCATGCT GGCTGAGGCC CAGGAAACCA TAATCAGCGG CCTGACCCCA 
GAGACCACCT ATTCCATTAC CGTCGCCGCC TACACCACCA AAGGGGATGG TGCCCGCAGC 
AAGCCCAAAG TTGTCACAAC GACCGGGGCA GTCCCAGGCC GGCCCACCAT GATGGTCAGC 
ACCACGGCCA TGAACACCGC ACTGCTGCAG TGGCACCCCC CCAAGGAGCT GCCAGGGGAG 
CTGCTGGGCT ACCGGCTACA GTACCACCGT GCCGATGAGG CAAAGCCCAA CATCCTAGAT 
TTTGGCAAGG ACGACCAGCA CTTTACAGTC ACCGGCCTAC ACAAGGGAGC CACCTACATT 
TTCCGGCTTG CCGCCAAGAA CCGGGCTGGC CCGGGAGAAG AGTTCGAGAA GGAGATCACG 
ACTCCCGAGG ATGCGCCCAG CGGCTTCCCC CAAAACCTGC GCGTGATAGG GCTGACCACC 
TCTACCACGG AGCTGACCTG GGACCCCCCG GTGCTGGCGG AGAGGAACGG GCGCATCACC 
AACTACTCCG TGGTGTACCG TGACATCAAC AGCCAGCAGG AGCTACAGAA CGTCACCGCC 
GACACCCACC TCATGCTCTC CGGCCTCAAG CCGGACACCA CTTACGACAT CAAGGTCCGC 
GCACGTACCA GCAAGGGCGC CGGCCCACTC AGCCCCAGCA TCCAGTCCCG GACCATGCCC 
ATGGAGCAAG TGTTTGCCAA GAACTTCCGT GTGGCGGCCG CTATGAAGAC GTCTGTGCTG 
CTCAGCTGGG AGGTCCCAGA CTCCTACAAG TCGGCTGTAC CCTTCAAGAT CCTGTACAAC 
GGGCAGAGTG TGGAGGTGGA CGGGCACTCC ATGCGGAAAC TGATCGCAGA CCTGCAGCCG 
AACACGGAGT ACTCGTTCGT GCTGATGAAC CGTGGCAGCA GCGCGGGGGG CCTGCAGCAC 
CTCGTGTCCA TCCGCACGGC CCCAGACCTC CTGCCACACA AGCCGATGCC CGCCTCCGCC 
TTCATAGAGG ATGGTCGCTT CACCCTCTCC ATGCCCCACG TGCAGGATGC CTCACTCATC 
AGGTGGTTCT ACATCGTGGT GGTACCCATC GACCGCGTGG GTGGGAGTGT GCTGGCCCCG 
AGATGGAGCA CGCCTGAGGA GCTGGAGCTG GACGAGCTTC TGGAGGCCAT CGAGCAGGGC 
GGTGGGGAGC GGCGACGACG GCGGCAGGCG GAGCGGCTGA AGCCATATGT GGCTGCACAG 
GTGGACGTGC TCCCCGAGAG CTTCACCCTA GGAGACAAGA AGAGCTACCG GGGCTTCTAC 
AACCGGCCTC TGTCTCCGGA CCTGAGCTAT CAGTGCTTTG TGCTCGCCTC CCTGAAGGAA 
CCCGTGGACC AGAAGCGCTA TGCCTCCAGC CCCTACTCTG ATGAGATCGT GGTCCAAGTG 
ACACCAGCCC AGCAGCAGGA GGAGCCTGAG ATGCTGTGGG TGACAGGCCC CGTCCTCGCA 
GTCATCCTCA TCATCCTCAT TGTCATCGCC ATCCTCCTGT TCAAGAGGAA GAGGACTCAC 
TCCCCATCAT CCAAGGACGA GCAGTCAATC GGGCTGAAGG ACTCCTTGCT GGCCCATTCC 
TCTGACCCTG TGGAGATGCG GAGGCTCAAC TACCAGACTC CAGGTATGCG AGACCACCCG 
CCCATCCCCA TCACTGACCT GGCCGACAAC ATTGAGCGCC TCAAAGCCAA CGACGGCCTC 
AAGTTCTCCC AGGAGTACGA GTCCATTGAC CCTGGACAGC AGTTCACCTG GGAGAATTCA 
AACCTGGAAG TGAACAAGCC CAAGAACCGC TATGCAAACG TCATCGCCTA CGACCACTCC 
CGAGTCATCC TTACCTCCGT CGATGGTGTC CCAGGGAGTG ACTATATCAA CGCCAACTAC 
ATCGATGGCT ACCGCAAGCA GAACGCCTAC ATTGCCACGC AGGGCCCACT GCCCGAGACC 
ATGGGCGATT TCTGGCGGAT GGTGTGGGAA CAGCGCACAG CCACCGTGGT CATGATGACA 
CGGCTGGAGG AGAAGTCTCG GGTGAAGTGT GATCAGTACT GGCCAGCCCG GGGTACCGAG 
ACCCACGGCC TCATTCAGGT GACCCTGCTG GACACGGTGG AGCTGGCCAC ATACACTGTG 
CGCACCTTTG CACTCCACAA GAGTGGCTCC AGCGAGAAGC GGGAGCTGCG TCAGTTCCAG 
TTCATGGCCT GGCCGGACCA CGGGGTTCCC GAGTACCCCA CCCCCATCCT GGCCTTCCTG 
CGGCGGGTCA AGGCCTGTAA CCCGCTAGAC GCAGGGCCCA TGGTGGTGCA CTGCAGCGCG 
GGCGTGGGCC GCACGGGCTG TTTCATCGTC ATCGACGCCA TGCTGGAGCG GATGAAGCAC 
GAGAAGACAG TGGACATCTA TGGCCACGTG ACATGCATGC GATCGCAGCG GAACTACATG 
GTGCAGACAG AGGACCAGTA CGTGTTCATC CACGAGGCGC TGCTAGAGGC CGCCACGTGC 
GGACACACGG AGGTGCCTGC CCGCAGCCTG TATGCCCATA TCCAGAAGCT GGGCCAAGTG 
CCTCCCGGGG AGAGTGTGAC TGCCATGGAG CTCGAGTTCA AGCTGCTGGC CAGCTCCAAG 
GCCCACACAT CCCGCTTCAT TAGCGCCAAC CTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTGAACATCA TGCCCTATGA GTTGACCCGT GTGTGTCTGC AGCCCATCCG TGGTGTGGAG 
GGCTCTGACT ACATCAATGC CAGCTTCTTG GACGGCTATA GGCAGCAGAA GGCCTACATC 
GCCACGCAGG GGCCCCTGGC AGAGAGCACC GAGGACTTCT GGCGCATGCT CTGGGAGCAC 
GGCTCCACCA TCGTCGTCAT GCTGACCAGG CTTCGGGAGA TGGGCAGGGA GAAATGCCAC 
CAGTACTGGC CAGCAGAGCG CTCCGCTCGC TACCAATACT TTGTCGTTGA CCCGATGGCT 
GAGTACAACA TGCCGCAGTA CATCCTGCGT GAGTTCAAGG TCACAGATGC CCGGGATGGG 
CAGTCGAGGA CAATCCGGCA GTTCCAGTTC ACAGACTGGC CCGAGCAGGG CGTGCCTAAG 
ACGGGTGAGG GCTTCATCGA CTTCATCGGG CAGGTGCACA AGACCAAGGA GCAGTTCGGG 
CAGGACGGGC CCATCACGGT GCACTGCAGT GCCGGCGTGG GCCGCACCGG GGTGTTCATC 
ACTCTGAGCA TCGTCCTGGA GCGGATGCGC TACGAGGGCG TGGTCGACAT GTTCCAGACC 
GTGAAGACCC TGCGCACACA GCGCCCAGCC ATGGTGCAGA CGGAGGACCA GTACCAGCTG 
TGCTATCGTG CGGCCCTGGA GTACCTTGGC AGCTTTGACC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_857873.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_862966.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X6, mRNA.

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CloneID	OCe17425
Clone ID Related Accession (Same CDS sequence)	XM_857873.4 , XM_857873.5
Accession Version	XM_857873.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_857873.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCACCCCCTC AGCCCCTCCC CAGAAGGTGA CCTGTGTGAG CACGGGCTCC 
ACCACGGTTC GGGTAAGTTG GGTCCCGCCG CCAGCTGCCA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCAGTGGAC GGCGAGGACC GAGGGCGGCA TGTGGTGGAG 
AGCATCAGTC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACTGACGTG GGCCCCGGCC CAGAGAGCAG CCCGGTGCTC 
GTGCGCACAG ACGAGGACGT GCCCAGTGGA CCGCCACGGA AGGTGGAGGT GGAACCACTG 
AATTCCACTG CTGTGCGTGT CTCTTGGAAG CTGCCCGTCC CCAGCAAGCA GCACGGCCAG 
ATTCGCGGCT ACCAGGTCAC CTATGTGCGG CTAGAGAATG GCGAGCCCCG TGGCCCACCC 
ATCATCCAGG ACGTCATGCT GGCTGAGGCC CAGGAAACCA TAATCAGCGG CCTGACCCCA 
GAGACCACCT ATTCCATTAC CGTCGCCGCC TACACCACCA AAGGGGATGG TGCCCGCAGC 
AAGCCCAAAG TTGTCACAAC GACCGGGGCA GTCCCAGGCC GGCCCACCAT GATGGTCAGC 
ACCACGGCCA TGAACACCGC ACTGCTGCAG TGGCACCCCC CCAAGGAGCT GCCAGGGGAG 
CTGCTGGGCT ACCGGCTACA GTACCACCGT GCCGATGAGG CAAAGCCCAA CATCCTAGAT 
TTTGGCAAGG ACGACCAGCA CTTTACAGTC ACCGGCCTAC ACAAGGGAGC CACCTACATT 
TTCCGGCTTG CCGCCAAGAA CCGGGCTGGC CCGGGAGAAG AGTTCGAGAA GGAGATCACG 
ACTCCCGAGG ATGCGCCCAG CGGCTTCCCC CAAAACCTGC GCGTGATAGG GCTGACCACC 
TCTACCACGG AGCTGACCTG GGACCCCCCG GTGCTGGCGG AGAGGAACGG GCGCATCACC 
AACTACTCCG TGGTGTACCG TGACATCAAC AGCCAGCAGG AGCTACAGAA CGTCACCGCC 
GACACCCACC TCATGCTCTC CGGCCTCAAG CCGGACACCA CTTACGACAT CAAGGTCCGC 
GCACGTACCA GCAAGGGCGC CGGCCCACTC AGCCCCAGCA TCCAGTCCCG GACCATGCCC 
ATGGAGCAAG TGTTTGCCAA GAACTTCCGT GTGGCGGCCG CTATGAAGAC GTCTGTGCTG 
CTCAGCTGGG AGGTCCCAGA CTCCTACAAG TCGGCTGTAC CCTTCAAGAT CCTGTACAAC 
GGGCAGAGTG TGGAGGTGGA CGGGCACTCC ATGCGGAAAC TGATCGCAGA CCTGCAGCCG 
AACACGGAGT ACTCGTTCGT GCTGATGAAC CGTGGCAGCA GCGCGGGGGG CCTGCAGCAC 
CTCGTGTCCA TCCGCACGGC CCCAGACCTC CTGCCACACA AGCCGATGCC CGCCTCCGCC 
TTCATAGAGG ATGGTCGCTT CACCCTCTCC ATGCCCCACG TGCAGGATGC CTCACTCATC 
AGGTGGTTCT ACATCGTGGT GGTACCCATC GACCGCGTGG GTGGGAGTGT GCTGGCCCCG 
AGATGGAGCA CGCCTGAGGA GCTGGAGCTG GACGAGCTTC TGGAGGCCAT CGAGCAGGGC 
GGTGGGGAGC GGCGACGACG GCGGCAGGCG GAGCGGCTGA AGCCATATGT GGCTGCACAG 
GTGGACGTGC TCCCCGAGAG CTTCACCCTA GGAGACAAGA AGAGCTACCG GGGCTTCTAC 
AACCGGCCTC TGTCTCCGGA CCTGAGCTAT CAGTGCTTTG TGCTCGCCTC CCTGAAGGAA 
CCCGTGGACC AGAAGCGCTA TGCCTCCAGC CCCTACTCTG ATGAGATCGT GGTCCAAGTG 
ACACCAGCCC AGCAGCAGGA GGAGCCTGAG ATGCTGTGGG TGACAGGCCC CGTCCTCGCA 
GTCATCCTCA TCATCCTCAT TGTCATCGCC ATCCTCCTGT TCAAGAGGAA GAGGACTCAC 
TCCCCATCAT CCAAGGACGA GCAGTCAATC GGGCTGAAGG ACTCCTTGCT GGCCCATTCC 
TCTGACCCTG TGGAGATGCG GAGGCTCAAC TACCAGACTC CAGGTATGCG AGACCACCCG 
CCCATCCCCA TCACTGACCT GGCCGACAAC ATTGAGCGCC TCAAAGCCAA CGACGGCCTC 
AAGTTCTCCC AGGAGTACGA GTCCATTGAC CCTGGACAGC AGTTCACCTG GGAGAATTCA 
AACCTGGAAG TGAACAAGCC CAAGAACCGC TATGCAAACG TCATCGCCTA CGACCACTCC 
CGAGTCATCC TTACCTCCGT CGATGGTGTC CCAGGGAGTG ACTATATCAA CGCCAACTAC 
ATCGATGGCT ACCGCAAGCA GAACGCCTAC ATTGCCACGC AGGGCCCACT GCCCGAGACC 
ATGGGCGATT TCTGGCGGAT GGTGTGGGAA CAGCGCACAG CCACCGTGGT CATGATGACA 
CGGCTGGAGG AGAAGTCTCG GGTGAAGTGT GATCAGTACT GGCCAGCCCG GGGTACCGAG 
ACCCACGGCC TCATTCAGGT GACCCTGCTG GACACGGTGG AGCTGGCCAC ATACACTGTG 
CGCACCTTTG CACTCCACAA GAGTGGCTCC AGCGAGAAGC GGGAGCTGCG TCAGTTCCAG 
TTCATGGCCT GGCCGGACCA CGGGGTTCCC GAGTACCCCA CCCCCATCCT GGCCTTCCTG 
CGGCGGGTCA AGGCCTGTAA CCCGCTAGAC GCAGGGCCCA TGGTGGTGCA CTGCAGCGCG 
GGCGTGGGCC GCACGGGCTG TTTCATCGTC ATCGACGCCA TGCTGGAGCG GATGAAGCAC 
GAGAAGACAG TGGACATCTA TGGCCACGTG ACATGCATGC GATCGCAGCG GAACTACATG 
GTGCAGACAG AGGACCAGTA CGTGTTCATC CACGAGGCGC TGCTAGAGGC CGCCACGTGC 
GGACACACGG AGGTGCCTGC CCGCAGCCTG TATGCCCATA TCCAGAAGCT GGGCCAAGTG 
CCTCCCGGGG AGAGTGTGAC TGCCATGGAG CTCGAGTTCA AGCTGCTGGC CAGCTCCAAG 
GCCCACACAT CCCGCTTCAT TAGCGCCAAC CTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTGAACATCA TGCCCTATGA GTTGACCCGT GTGTGTCTGC AGCCCATCCG TGGTGTGGAG 
GGCTCTGACT ACATCAATGC CAGCTTCTTG GACGGCTATA GGCAGCAGAA GGCCTACATC 
GCCACGCAGG GGCCCCTGGC AGAGAGCACC GAGGACTTCT GGCGCATGCT CTGGGAGCAC 
GGCTCCACCA TCGTCGTCAT GCTGACCAGG CTTCGGGAGA TGGGCAGGGA GAAATGCCAC 
CAGTACTGGC CAGCAGAGCG CTCCGCTCGC TACCAATACT TTGTCGTTGA CCCGATGGCT 
GAGTACAACA TGCCGCAGTA CATCCTGCGT GAGTTCAAGG TCACAGATGC CCGGGATGGG 
CAGTCGAGGA CAATCCGGCA GTTCCAGTTC ACAGACTGGC CCGAGCAGGG CGTGCCTAAG 
ACGGGTGAGG GCTTCATCGA CTTCATCGGG CAGGTGCACA AGACCAAGGA GCAGTTCGGG 
CAGGACGGGC CCATCACGGT GCACTGCAGT GCCGGCGTGG GCCGCACCGG GGTGTTCATC 
ACTCTGAGCA TCGTCCTGGA GCGGATGCGC TACGAGGGCG TGGTCGACAT GTTCCAGACC 
GTGAAGACCC TGCGCACACA GCGCCCAGCC ATGGTGCAGA CGGAGGACCA GTACCAGCTG 
TGCTATCGTG CGGCCCTGGA GTACCTTGGC AGCTTTGACC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_862966.1
CDS	125..5818
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPAASRNGVITQYSVAYEAVDGEDRGRHVVESISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_857873.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_857873.5

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCACCCCCTC AGCCCCTCCC CAGAAGGTGA CCTGTGTGAG CACGGGCTCC 
ACCACGGTTC GGGTAAGTTG GGTCCCGCCG CCAGCTGCCA GCCGCAACGG CGTCATCACC 
CAGTACTCGG TGGCCTACGA GGCAGTGGAC GGCGAGGACC GAGGGCGGCA TGTGGTGGAG 
AGCATCAGTC GCGAGCACTC CAGCTGGGAC CTGGTGGGCC TGGAGAAGTG GACGGAGTAC 
CGGGTGTGGG TGCGGGCGCA CACTGACGTG GGCCCCGGCC CAGAGAGCAG CCCGGTGCTC 
GTGCGCACAG ACGAGGACGT GCCCAGTGGA CCGCCACGGA AGGTGGAGGT GGAACCACTG 
AATTCCACTG CTGTGCGTGT CTCTTGGAAG CTGCCCGTCC CCAGCAAGCA GCACGGCCAG 
ATTCGCGGCT ACCAGGTCAC CTATGTGCGG CTAGAGAATG GCGAGCCCCG TGGCCCACCC 
ATCATCCAGG ACGTCATGCT GGCTGAGGCC CAGGAAACCA TAATCAGCGG CCTGACCCCA 
GAGACCACCT ATTCCATTAC CGTCGCCGCC TACACCACCA AAGGGGATGG TGCCCGCAGC 
AAGCCCAAAG TTGTCACAAC GACCGGGGCA GTCCCAGGCC GGCCCACCAT GATGGTCAGC 
ACCACGGCCA TGAACACCGC ACTGCTGCAG TGGCACCCCC CCAAGGAGCT GCCAGGGGAG 
CTGCTGGGCT ACCGGCTACA GTACCACCGT GCCGATGAGG CAAAGCCCAA CATCCTAGAT 
TTTGGCAAGG ACGACCAGCA CTTTACAGTC ACCGGCCTAC ACAAGGGAGC CACCTACATT 
TTCCGGCTTG CCGCCAAGAA CCGGGCTGGC CCGGGAGAAG AGTTCGAGAA GGAGATCACG 
ACTCCCGAGG ATGCGCCCAG CGGCTTCCCC CAAAACCTGC GCGTGATAGG GCTGACCACC 
TCTACCACGG AGCTGACCTG GGACCCCCCG GTGCTGGCGG AGAGGAACGG GCGCATCACC 
AACTACTCCG TGGTGTACCG TGACATCAAC AGCCAGCAGG AGCTACAGAA CGTCACCGCC 
GACACCCACC TCATGCTCTC CGGCCTCAAG CCGGACACCA CTTACGACAT CAAGGTCCGC 
GCACGTACCA GCAAGGGCGC CGGCCCACTC AGCCCCAGCA TCCAGTCCCG GACCATGCCC 
ATGGAGCAAG TGTTTGCCAA GAACTTCCGT GTGGCGGCCG CTATGAAGAC GTCTGTGCTG 
CTCAGCTGGG AGGTCCCAGA CTCCTACAAG TCGGCTGTAC CCTTCAAGAT CCTGTACAAC 
GGGCAGAGTG TGGAGGTGGA CGGGCACTCC ATGCGGAAAC TGATCGCAGA CCTGCAGCCG 
AACACGGAGT ACTCGTTCGT GCTGATGAAC CGTGGCAGCA GCGCGGGGGG CCTGCAGCAC 
CTCGTGTCCA TCCGCACGGC CCCAGACCTC CTGCCACACA AGCCGATGCC CGCCTCCGCC 
TTCATAGAGG ATGGTCGCTT CACCCTCTCC ATGCCCCACG TGCAGGATGC CTCACTCATC 
AGGTGGTTCT ACATCGTGGT GGTACCCATC GACCGCGTGG GTGGGAGTGT GCTGGCCCCG 
AGATGGAGCA CGCCTGAGGA GCTGGAGCTG GACGAGCTTC TGGAGGCCAT CGAGCAGGGC 
GGTGGGGAGC GGCGACGACG GCGGCAGGCG GAGCGGCTGA AGCCATATGT GGCTGCACAG 
GTGGACGTGC TCCCCGAGAG CTTCACCCTA GGAGACAAGA AGAGCTACCG GGGCTTCTAC 
AACCGGCCTC TGTCTCCGGA CCTGAGCTAT CAGTGCTTTG TGCTCGCCTC CCTGAAGGAA 
CCCGTGGACC AGAAGCGCTA TGCCTCCAGC CCCTACTCTG ATGAGATCGT GGTCCAAGTG 
ACACCAGCCC AGCAGCAGGA GGAGCCTGAG ATGCTGTGGG TGACAGGCCC CGTCCTCGCA 
GTCATCCTCA TCATCCTCAT TGTCATCGCC ATCCTCCTGT TCAAGAGGAA GAGGACTCAC 
TCCCCATCAT CCAAGGACGA GCAGTCAATC GGGCTGAAGG ACTCCTTGCT GGCCCATTCC 
TCTGACCCTG TGGAGATGCG GAGGCTCAAC TACCAGACTC CAGGTATGCG AGACCACCCG 
CCCATCCCCA TCACTGACCT GGCCGACAAC ATTGAGCGCC TCAAAGCCAA CGACGGCCTC 
AAGTTCTCCC AGGAGTACGA GTCCATTGAC CCTGGACAGC AGTTCACCTG GGAGAATTCA 
AACCTGGAAG TGAACAAGCC CAAGAACCGC TATGCAAACG TCATCGCCTA CGACCACTCC 
CGAGTCATCC TTACCTCCGT CGATGGTGTC CCAGGGAGTG ACTATATCAA CGCCAACTAC 
ATCGATGGCT ACCGCAAGCA GAACGCCTAC ATTGCCACGC AGGGCCCACT GCCCGAGACC 
ATGGGCGATT TCTGGCGGAT GGTGTGGGAA CAGCGCACAG CCACCGTGGT CATGATGACA 
CGGCTGGAGG AGAAGTCTCG GGTGAAGTGT GATCAGTACT GGCCAGCCCG GGGTACCGAG 
ACCCACGGCC TCATTCAGGT GACCCTGCTG GACACGGTGG AGCTGGCCAC ATACACTGTG 
CGCACCTTTG CACTCCACAA GAGTGGCTCC AGCGAGAAGC GGGAGCTGCG TCAGTTCCAG 
TTCATGGCCT GGCCGGACCA CGGGGTTCCC GAGTACCCCA CCCCCATCCT GGCCTTCCTG 
CGGCGGGTCA AGGCCTGTAA CCCGCTAGAC GCAGGGCCCA TGGTGGTGCA CTGCAGCGCG 
GGCGTGGGCC GCACGGGCTG TTTCATCGTC ATCGACGCCA TGCTGGAGCG GATGAAGCAC 
GAGAAGACAG TGGACATCTA TGGCCACGTG ACATGCATGC GATCGCAGCG GAACTACATG 
GTGCAGACAG AGGACCAGTA CGTGTTCATC CACGAGGCGC TGCTAGAGGC CGCCACGTGC 
GGACACACGG AGGTGCCTGC CCGCAGCCTG TATGCCCATA TCCAGAAGCT GGGCCAAGTG 
CCTCCCGGGG AGAGTGTGAC TGCCATGGAG CTCGAGTTCA AGCTGCTGGC CAGCTCCAAG 
GCCCACACAT CCCGCTTCAT TAGCGCCAAC CTGCCCTGCA ACAAGTTCAA GAACCGCCTG 
GTGAACATCA TGCCCTATGA GTTGACCCGT GTGTGTCTGC AGCCCATCCG TGGTGTGGAG 
GGCTCTGACT ACATCAATGC CAGCTTCTTG GACGGCTATA GGCAGCAGAA GGCCTACATC 
GCCACGCAGG GGCCCCTGGC AGAGAGCACC GAGGACTTCT GGCGCATGCT CTGGGAGCAC 
GGCTCCACCA TCGTCGTCAT GCTGACCAGG CTTCGGGAGA TGGGCAGGGA GAAATGCCAC 
CAGTACTGGC CAGCAGAGCG CTCCGCTCGC TACCAATACT TTGTCGTTGA CCCGATGGCT 
GAGTACAACA TGCCGCAGTA CATCCTGCGT GAGTTCAAGG TCACAGATGC CCGGGATGGG 
CAGTCGAGGA CAATCCGGCA GTTCCAGTTC ACAGACTGGC CCGAGCAGGG CGTGCCTAAG 
ACGGGTGAGG GCTTCATCGA CTTCATCGGG CAGGTGCACA AGACCAAGGA GCAGTTCGGG 
CAGGACGGGC CCATCACGGT GCACTGCAGT GCCGGCGTGG GCCGCACCGG GGTGTTCATC 
ACTCTGAGCA TCGTCCTGGA GCGGATGCGC TACGAGGGCG TGGTCGACAT GTTCCAGACC 
GTGAAGACCC TGCGCACACA GCGCCCAGCC ATGGTGCAGA CGGAGGACCA GTACCAGCTG 
TGCTATCGTG CGGCCCTGGA GTACCTTGGC AGCTTTGACC ACTATGCAAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_005629115.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629172.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X7, mRNA.

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CloneID	OCe17426
Clone ID Related Accession (Same CDS sequence)	XM_005629115.2 , XM_005629115.3
Accession Version	XM_005629115.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629115.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ATGCCCAGTG GACCGCCACG GAAGGTGGAG 
GTGGAACCAC TGAATTCCAC TGCTGTGCGT GTCTCTTGGA AGCTGCCCGT CCCCAGCAAG 
CAGCACGGCC AGATTCGCGG CTACCAGGTC ACCTATGTGC GGCTAGAGAA TGGCGAGCCC 
CGTGGCCCAC CCATCATCCA GGACGTCATG CTGGCTGAGG CCCAGTGGCG GCCAGAGGAG 
TCCGAGGACT ATGAAACCAT AATCAGCGGC CTGACCCCAG AGACCACCTA TTCCATTACC 
GTCGCCGCCT ACACCACCAA AGGGGATGGT GCCCGCAGCA AGCCCAAAGT TGTCACAACG 
ACCGGGGCAG TCCCAGGCCG GCCCACCATG ATGGTCAGCA CCACGGCCAT GAACACCGCA 
CTGCTGCAGT GGCACCCCCC CAAGGAGCTG CCAGGGGAGC TGCTGGGCTA CCGGCTACAG 
TACCACCGTG CCGATGAGGC AAAGCCCAAC ATCCTAGATT TTGGCAAGGA CGACCAGCAC 
TTTACAGTCA CCGGCCTACA CAAGGGAGCC ACCTACATTT TCCGGCTTGC CGCCAAGAAC 
CGGGCTGGCC CGGGAGAAGA GTTCGAGAAG GAGATCACGA CTCCCGAGGA TGCGCCCAGC 
GGCTTCCCCC AAAACCTGCG CGTGATAGGG CTGACCACCT CTACCACGGA GCTGACCTGG 
GACCCCCCGG TGCTGGCGGA GAGGAACGGG CGCATCACCA ACTACTCCGT GGTGTACCGT 
GACATCAACA GCCAGCAGGA GCTACAGAAC GTCACCGCCG ACACCCACCT CATGCTCTCC 
GGCCTCAAGC CGGACACCAC TTACGACATC AAGGTCCGCG CACGTACCAG CAAGGGCGCC 
GGCCCACTCA GCCCCAGCAT CCAGTCCCGG ACCATGCCCA TGGAGCAAGT GTTTGCCAAG 
AACTTCCGTG TGGCGGCCGC TATGAAGACG TCTGTGCTGC TCAGCTGGGA GGTCCCAGAC 
TCCTACAAGT CGGCTGTACC CTTCAAGATC CTGTACAACG GGCAGAGTGT GGAGGTGGAC 
GGGCACTCCA TGCGGAAACT GATCGCAGAC CTGCAGCCGA ACACGGAGTA CTCGTTCGTG 
CTGATGAACC GTGGCAGCAG CGCGGGGGGC CTGCAGCACC TCGTGTCCAT CCGCACGGCC 
CCAGACCTCC TGCCACACAA GCCGATGCCC GCCTCCGCCT TCATAGAGGA TGGTCGCTTC 
ACCCTCTCCA TGCCCCACGT GCAGGATGCC TCACTCATCA GGTGGTTCTA CATCGTGGTG 
GTACCCATCG ACCGCGTGGG TGGGAGTGTG CTGGCCCCGA GATGGAGCAC GCCTGAGGAG 
CTGGAGCTGG ACGAGCTTCT GGAGGCCATC GAGCAGGGCG GTGGGGAGCG GCGACGACGG 
CGGCAGGCGG AGCGGCTGAA GCCATATGTG GCTGCACAGG TGGACGTGCT CCCCGAGAGC 
TTCACCCTAG GAGACAAGAA GAGCTACCGG GGCTTCTACA ACCGGCCTCT GTCTCCGGAC 
CTGAGCTATC AGTGCTTTGT GCTCGCCTCC CTGAAGGAAC CCGTGGACCA GAAGCGCTAT 
GCCTCCAGCC CCTACTCTGA TGAGATCGTG GTCCAAGTGA CACCAGCCCA GCAGCAGGAG 
GAGCCTGAGA TGCTGTGGGT GACAGGCCCC GTCCTCGCAG TCATCCTCAT CATCCTCATT 
GTCATCGCCA TCCTCCTGTT CAAGAGGAAG AGGACTCACT CCCCATCATC CAAGGACGAG 
CAGTCAATCG GGCTGAAGGA CTCCTTGCTG GCCCATTCCT CTGACCCTGT GGAGATGCGG 
AGGCTCAACT ACCAGACTCC AGGTTCCAGT GTCCCCAGTT GCCCGAATAT CTCAAGTATG 
CGAGACCACC CGCCCATCCC CATCACTGAC CTGGCCGACA ACATTGAGCG CCTCAAAGCC 
AACGACGGCC TCAAGTTCTC CCAGGAGTAC GAGTCCATTG ACCCTGGACA GCAGTTCACC 
TGGGAGAATT CAAACCTGGA AGTGAACAAG CCCAAGAACC GCTATGCAAA CGTCATCGCC 
TACGACCACT CCCGAGTCAT CCTTACCTCC GTCGATGGTG TCCCAGGGAG TGACTATATC 
AACGCCAACT ACATCGATGG CTACCGCAAG CAGAACGCCT ACATTGCCAC GCAGGGCCCA 
CTGCCCGAGA CCATGGGCGA TTTCTGGCGG ATGGTGTGGG AACAGCGCAC AGCCACCGTG 
GTCATGATGA CACGGCTGGA GGAGAAGTCT CGGGTGAAGT GTGATCAGTA CTGGCCAGCC 
CGGGGTACCG AGACCCACGG CCTCATTCAG GTGACCCTGC TGGACACGGT GGAGCTGGCC 
ACATACACTG TGCGCACCTT TGCACTCCAC AAGAGTGGCT CCAGCGAGAA GCGGGAGCTG 
CGTCAGTTCC AGTTCATGGC CTGGCCGGAC CACGGGGTTC CCGAGTACCC CACCCCCATC 
CTGGCCTTCC TGCGGCGGGT CAAGGCCTGT AACCCGCTAG ACGCAGGGCC CATGGTGGTG 
CACTGCAGCG CGGGCGTGGG CCGCACGGGC TGTTTCATCG TCATCGACGC CATGCTGGAG 
CGGATGAAGC ACGAGAAGAC AGTGGACATC TATGGCCACG TGACATGCAT GCGATCGCAG 
CGGAACTACA TGGTGCAGAC AGAGGACCAG TACGTGTTCA TCCACGAGGC GCTGCTAGAG 
GCCGCCACGT GCGGACACAC GGAGGTGCCT GCCCGCAGCC TGTATGCCCA TATCCAGAAG 
CTGGGCCAAG TGCCTCCCGG GGAGAGTGTG ACTGCCATGG AGCTCGAGTT CAAGCTGCTG 
GCCAGCTCCA AGGCCCACAC ATCCCGCTTC ATTAGCGCCA ACCTGCCCTG CAACAAGTTC 
AAGAACCGCC TGGTGAACAT CATGCCCTAT GAGTTGACCC GTGTGTGTCT GCAGCCCATC 
CGTGGTGTGG AGGGCTCTGA CTACATCAAT GCCAGCTTCT TGGACGGCTA TAGGCAGCAG 
AAGGCCTACA TCGCCACGCA GGGGCCCCTG GCAGAGAGCA CCGAGGACTT CTGGCGCATG 
CTCTGGGAGC ACGGCTCCAC CATCGTCGTC ATGCTGACCA GGCTTCGGGA GATGGGCAGG 
GAGAAATGCC ACCAGTACTG GCCAGCAGAG CGCTCCGCTC GCTACCAATA CTTTGTCGTT 
GACCCGATGG CTGAGTACAA CATGCCGCAG TACATCCTGC GTGAGTTCAA GGTCACAGAT 
GCCCGGGATG GGCAGTCGAG GACAATCCGG CAGTTCCAGT TCACAGACTG GCCCGAGCAG 
GGCGTGCCTA AGACGGGTGA GGGCTTCATC GACTTCATCG GGCAGGTGCA CAAGACCAAG 
GAGCAGTTCG GGCAGGACGG GCCCATCACG GTGCACTGCA GTGCCGGCGT GGGCCGCACC 
GGGGTGTTCA TCACTCTGAG CATCGTCCTG GAGCGGATGC GCTACGAGGG CGTGGTCGAC 
ATGTTCCAGA CCGTGAAGAC CCTGCGCACA CAGCGCCCAG CCATGGTGCA GACGGAGGAC 
CAGTACCAGC TGTGCTATCG TGCGGCCCTG GAGTACCTTG GCAGCTTTGA CCACTATGCA 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629172.1
CDS	125..5590
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGGSPIRGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSMPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQWRPEESEDYETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGSSVPSCPNISSMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_005629115.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629115.2

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ATGCCCAGTG GACCGCCACG GAAGGTGGAG 
GTGGAACCAC TGAATTCCAC TGCTGTGCGT GTCTCTTGGA AGCTGCCCGT CCCCAGCAAG 
CAGCACGGCC AGATTCGCGG CTACCAGGTC ACCTATGTGC GGCTAGAGAA TGGCGAGCCC 
CGTGGCCCAC CCATCATCCA GGACGTCATG CTGGCTGAGG CCCAGTGGCG GCCAGAGGAG 
TCCGAGGACT ATGAAACCAT AATCAGCGGC CTGACCCCAG AGACCACCTA TTCCATTACC 
GTCGCCGCCT ACACCACCAA AGGGGATGGT GCCCGCAGCA AGCCCAAAGT TGTCACAACG 
ACCGGGGCAG TCCCAGGCCG GCCCACCATG ATGGTCAGCA CCACGGCCAT GAACACCGCA 
CTGCTGCAGT GGCACCCCCC CAAGGAGCTG CCAGGGGAGC TGCTGGGCTA CCGGCTACAG 
TACCACCGTG CCGATGAGGC AAAGCCCAAC ATCCTAGATT TTGGCAAGGA CGACCAGCAC 
TTTACAGTCA CCGGCCTACA CAAGGGAGCC ACCTACATTT TCCGGCTTGC CGCCAAGAAC 
CGGGCTGGCC CGGGAGAAGA GTTCGAGAAG GAGATCACGA CTCCCGAGGA TGCGCCCAGC 
GGCTTCCCCC AAAACCTGCG CGTGATAGGG CTGACCACCT CTACCACGGA GCTGACCTGG 
GACCCCCCGG TGCTGGCGGA GAGGAACGGG CGCATCACCA ACTACTCCGT GGTGTACCGT 
GACATCAACA GCCAGCAGGA GCTACAGAAC GTCACCGCCG ACACCCACCT CATGCTCTCC 
GGCCTCAAGC CGGACACCAC TTACGACATC AAGGTCCGCG CACGTACCAG CAAGGGCGCC 
GGCCCACTCA GCCCCAGCAT CCAGTCCCGG ACCATGCCCA TGGAGCAAGT GTTTGCCAAG 
AACTTCCGTG TGGCGGCCGC TATGAAGACG TCTGTGCTGC TCAGCTGGGA GGTCCCAGAC 
TCCTACAAGT CGGCTGTACC CTTCAAGATC CTGTACAACG GGCAGAGTGT GGAGGTGGAC 
GGGCACTCCA TGCGGAAACT GATCGCAGAC CTGCAGCCGA ACACGGAGTA CTCGTTCGTG 
CTGATGAACC GTGGCAGCAG CGCGGGGGGC CTGCAGCACC TCGTGTCCAT CCGCACGGCC 
CCAGACCTCC TGCCACACAA GCCGATGCCC GCCTCCGCCT TCATAGAGGA TGGTCGCTTC 
ACCCTCTCCA TGCCCCACGT GCAGGATGCC TCACTCATCA GGTGGTTCTA CATCGTGGTG 
GTACCCATCG ACCGCGTGGG TGGGAGTGTG CTGGCCCCGA GATGGAGCAC GCCTGAGGAG 
CTGGAGCTGG ACGAGCTTCT GGAGGCCATC GAGCAGGGCG GTGGGGAGCG GCGACGACGG 
CGGCAGGCGG AGCGGCTGAA GCCATATGTG GCTGCACAGG TGGACGTGCT CCCCGAGAGC 
TTCACCCTAG GAGACAAGAA GAGCTACCGG GGCTTCTACA ACCGGCCTCT GTCTCCGGAC 
CTGAGCTATC AGTGCTTTGT GCTCGCCTCC CTGAAGGAAC CCGTGGACCA GAAGCGCTAT 
GCCTCCAGCC CCTACTCTGA TGAGATCGTG GTCCAAGTGA CACCAGCCCA GCAGCAGGAG 
GAGCCTGAGA TGCTGTGGGT GACAGGCCCC GTCCTCGCAG TCATCCTCAT CATCCTCATT 
GTCATCGCCA TCCTCCTGTT CAAGAGGAAG AGGACTCACT CCCCATCATC CAAGGACGAG 
CAGTCAATCG GGCTGAAGGA CTCCTTGCTG GCCCATTCCT CTGACCCTGT GGAGATGCGG 
AGGCTCAACT ACCAGACTCC AGGTTCCAGT GTCCCCAGTT GCCCGAATAT CTCAAGTATG 
CGAGACCACC CGCCCATCCC CATCACTGAC CTGGCCGACA ACATTGAGCG CCTCAAAGCC 
AACGACGGCC TCAAGTTCTC CCAGGAGTAC GAGTCCATTG ACCCTGGACA GCAGTTCACC 
TGGGAGAATT CAAACCTGGA AGTGAACAAG CCCAAGAACC GCTATGCAAA CGTCATCGCC 
TACGACCACT CCCGAGTCAT CCTTACCTCC GTCGATGGTG TCCCAGGGAG TGACTATATC 
AACGCCAACT ACATCGATGG CTACCGCAAG CAGAACGCCT ACATTGCCAC GCAGGGCCCA 
CTGCCCGAGA CCATGGGCGA TTTCTGGCGG ATGGTGTGGG AACAGCGCAC AGCCACCGTG 
GTCATGATGA CACGGCTGGA GGAGAAGTCT CGGGTGAAGT GTGATCAGTA CTGGCCAGCC 
CGGGGTACCG AGACCCACGG CCTCATTCAG GTGACCCTGC TGGACACGGT GGAGCTGGCC 
ACATACACTG TGCGCACCTT TGCACTCCAC AAGAGTGGCT CCAGCGAGAA GCGGGAGCTG 
CGTCAGTTCC AGTTCATGGC CTGGCCGGAC CACGGGGTTC CCGAGTACCC CACCCCCATC 
CTGGCCTTCC TGCGGCGGGT CAAGGCCTGT AACCCGCTAG ACGCAGGGCC CATGGTGGTG 
CACTGCAGCG CGGGCGTGGG CCGCACGGGC TGTTTCATCG TCATCGACGC CATGCTGGAG 
CGGATGAAGC ACGAGAAGAC AGTGGACATC TATGGCCACG TGACATGCAT GCGATCGCAG 
CGGAACTACA TGGTGCAGAC AGAGGACCAG TACGTGTTCA TCCACGAGGC GCTGCTAGAG 
GCCGCCACGT GCGGACACAC GGAGGTGCCT GCCCGCAGCC TGTATGCCCA TATCCAGAAG 
CTGGGCCAAG TGCCTCCCGG GGAGAGTGTG ACTGCCATGG AGCTCGAGTT CAAGCTGCTG 
GCCAGCTCCA AGGCCCACAC ATCCCGCTTC ATTAGCGCCA ACCTGCCCTG CAACAAGTTC 
AAGAACCGCC TGGTGAACAT CATGCCCTAT GAGTTGACCC GTGTGTGTCT GCAGCCCATC 
CGTGGTGTGG AGGGCTCTGA CTACATCAAT GCCAGCTTCT TGGACGGCTA TAGGCAGCAG 
AAGGCCTACA TCGCCACGCA GGGGCCCCTG GCAGAGAGCA CCGAGGACTT CTGGCGCATG 
CTCTGGGAGC ACGGCTCCAC CATCGTCGTC ATGCTGACCA GGCTTCGGGA GATGGGCAGG 
GAGAAATGCC ACCAGTACTG GCCAGCAGAG CGCTCCGCTC GCTACCAATA CTTTGTCGTT 
GACCCGATGG CTGAGTACAA CATGCCGCAG TACATCCTGC GTGAGTTCAA GGTCACAGAT 
GCCCGGGATG GGCAGTCGAG GACAATCCGG CAGTTCCAGT TCACAGACTG GCCCGAGCAG 
GGCGTGCCTA AGACGGGTGA GGGCTTCATC GACTTCATCG GGCAGGTGCA CAAGACCAAG 
GAGCAGTTCG GGCAGGACGG GCCCATCACG GTGCACTGCA GTGCCGGCGT GGGCCGCACC 
GGGGTGTTCA TCACTCTGAG CATCGTCCTG GAGCGGATGC GCTACGAGGG CGTGGTCGAC 
ATGTTCCAGA CCGTGAAGAC CCTGCGCACA CAGCGCCCAG CCATGGTGCA GACGGAGGAC 
CAGTACCAGC TGTGCTATCG TGCGGCCCTG GAGTACCTTG GCAGCTTTGA CCACTATGCA 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_005629115.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629172.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X8, mRNA.

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CloneID	OCe17426
Clone ID Related Accession (Same CDS sequence)	XM_005629115.2 , XM_005629115.3
Accession Version	XM_005629115.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629115.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ATGCCCAGTG GACCGCCACG GAAGGTGGAG 
GTGGAACCAC TGAATTCCAC TGCTGTGCGT GTCTCTTGGA AGCTGCCCGT CCCCAGCAAG 
CAGCACGGCC AGATTCGCGG CTACCAGGTC ACCTATGTGC GGCTAGAGAA TGGCGAGCCC 
CGTGGCCCAC CCATCATCCA GGACGTCATG CTGGCTGAGG CCCAGTGGCG GCCAGAGGAG 
TCCGAGGACT ATGAAACCAT AATCAGCGGC CTGACCCCAG AGACCACCTA TTCCATTACC 
GTCGCCGCCT ACACCACCAA AGGGGATGGT GCCCGCAGCA AGCCCAAAGT TGTCACAACG 
ACCGGGGCAG TCCCAGGCCG GCCCACCATG ATGGTCAGCA CCACGGCCAT GAACACCGCA 
CTGCTGCAGT GGCACCCCCC CAAGGAGCTG CCAGGGGAGC TGCTGGGCTA CCGGCTACAG 
TACCACCGTG CCGATGAGGC AAAGCCCAAC ATCCTAGATT TTGGCAAGGA CGACCAGCAC 
TTTACAGTCA CCGGCCTACA CAAGGGAGCC ACCTACATTT TCCGGCTTGC CGCCAAGAAC 
CGGGCTGGCC CGGGAGAAGA GTTCGAGAAG GAGATCACGA CTCCCGAGGA TGCGCCCAGC 
GGCTTCCCCC AAAACCTGCG CGTGATAGGG CTGACCACCT CTACCACGGA GCTGACCTGG 
GACCCCCCGG TGCTGGCGGA GAGGAACGGG CGCATCACCA ACTACTCCGT GGTGTACCGT 
GACATCAACA GCCAGCAGGA GCTACAGAAC GTCACCGCCG ACACCCACCT CATGCTCTCC 
GGCCTCAAGC CGGACACCAC TTACGACATC AAGGTCCGCG CACGTACCAG CAAGGGCGCC 
GGCCCACTCA GCCCCAGCAT CCAGTCCCGG ACCATGCCCA TGGAGCAAGT GTTTGCCAAG 
AACTTCCGTG TGGCGGCCGC TATGAAGACG TCTGTGCTGC TCAGCTGGGA GGTCCCAGAC 
TCCTACAAGT CGGCTGTACC CTTCAAGATC CTGTACAACG GGCAGAGTGT GGAGGTGGAC 
GGGCACTCCA TGCGGAAACT GATCGCAGAC CTGCAGCCGA ACACGGAGTA CTCGTTCGTG 
CTGATGAACC GTGGCAGCAG CGCGGGGGGC CTGCAGCACC TCGTGTCCAT CCGCACGGCC 
CCAGACCTCC TGCCACACAA GCCGATGCCC GCCTCCGCCT TCATAGAGGA TGGTCGCTTC 
ACCCTCTCCA TGCCCCACGT GCAGGATGCC TCACTCATCA GGTGGTTCTA CATCGTGGTG 
GTACCCATCG ACCGCGTGGG TGGGAGTGTG CTGGCCCCGA GATGGAGCAC GCCTGAGGAG 
CTGGAGCTGG ACGAGCTTCT GGAGGCCATC GAGCAGGGCG GTGGGGAGCG GCGACGACGG 
CGGCAGGCGG AGCGGCTGAA GCCATATGTG GCTGCACAGG TGGACGTGCT CCCCGAGAGC 
TTCACCCTAG GAGACAAGAA GAGCTACCGG GGCTTCTACA ACCGGCCTCT GTCTCCGGAC 
CTGAGCTATC AGTGCTTTGT GCTCGCCTCC CTGAAGGAAC CCGTGGACCA GAAGCGCTAT 
GCCTCCAGCC CCTACTCTGA TGAGATCGTG GTCCAAGTGA CACCAGCCCA GCAGCAGGAG 
GAGCCTGAGA TGCTGTGGGT GACAGGCCCC GTCCTCGCAG TCATCCTCAT CATCCTCATT 
GTCATCGCCA TCCTCCTGTT CAAGAGGAAG AGGACTCACT CCCCATCATC CAAGGACGAG 
CAGTCAATCG GGCTGAAGGA CTCCTTGCTG GCCCATTCCT CTGACCCTGT GGAGATGCGG 
AGGCTCAACT ACCAGACTCC AGGTTCCAGT GTCCCCAGTT GCCCGAATAT CTCAAGTATG 
CGAGACCACC CGCCCATCCC CATCACTGAC CTGGCCGACA ACATTGAGCG CCTCAAAGCC 
AACGACGGCC TCAAGTTCTC CCAGGAGTAC GAGTCCATTG ACCCTGGACA GCAGTTCACC 
TGGGAGAATT CAAACCTGGA AGTGAACAAG CCCAAGAACC GCTATGCAAA CGTCATCGCC 
TACGACCACT CCCGAGTCAT CCTTACCTCC GTCGATGGTG TCCCAGGGAG TGACTATATC 
AACGCCAACT ACATCGATGG CTACCGCAAG CAGAACGCCT ACATTGCCAC GCAGGGCCCA 
CTGCCCGAGA CCATGGGCGA TTTCTGGCGG ATGGTGTGGG AACAGCGCAC AGCCACCGTG 
GTCATGATGA CACGGCTGGA GGAGAAGTCT CGGGTGAAGT GTGATCAGTA CTGGCCAGCC 
CGGGGTACCG AGACCCACGG CCTCATTCAG GTGACCCTGC TGGACACGGT GGAGCTGGCC 
ACATACACTG TGCGCACCTT TGCACTCCAC AAGAGTGGCT CCAGCGAGAA GCGGGAGCTG 
CGTCAGTTCC AGTTCATGGC CTGGCCGGAC CACGGGGTTC CCGAGTACCC CACCCCCATC 
CTGGCCTTCC TGCGGCGGGT CAAGGCCTGT AACCCGCTAG ACGCAGGGCC CATGGTGGTG 
CACTGCAGCG CGGGCGTGGG CCGCACGGGC TGTTTCATCG TCATCGACGC CATGCTGGAG 
CGGATGAAGC ACGAGAAGAC AGTGGACATC TATGGCCACG TGACATGCAT GCGATCGCAG 
CGGAACTACA TGGTGCAGAC AGAGGACCAG TACGTGTTCA TCCACGAGGC GCTGCTAGAG 
GCCGCCACGT GCGGACACAC GGAGGTGCCT GCCCGCAGCC TGTATGCCCA TATCCAGAAG 
CTGGGCCAAG TGCCTCCCGG GGAGAGTGTG ACTGCCATGG AGCTCGAGTT CAAGCTGCTG 
GCCAGCTCCA AGGCCCACAC ATCCCGCTTC ATTAGCGCCA ACCTGCCCTG CAACAAGTTC 
AAGAACCGCC TGGTGAACAT CATGCCCTAT GAGTTGACCC GTGTGTGTCT GCAGCCCATC 
CGTGGTGTGG AGGGCTCTGA CTACATCAAT GCCAGCTTCT TGGACGGCTA TAGGCAGCAG 
AAGGCCTACA TCGCCACGCA GGGGCCCCTG GCAGAGAGCA CCGAGGACTT CTGGCGCATG 
CTCTGGGAGC ACGGCTCCAC CATCGTCGTC ATGCTGACCA GGCTTCGGGA GATGGGCAGG 
GAGAAATGCC ACCAGTACTG GCCAGCAGAG CGCTCCGCTC GCTACCAATA CTTTGTCGTT 
GACCCGATGG CTGAGTACAA CATGCCGCAG TACATCCTGC GTGAGTTCAA GGTCACAGAT 
GCCCGGGATG GGCAGTCGAG GACAATCCGG CAGTTCCAGT TCACAGACTG GCCCGAGCAG 
GGCGTGCCTA AGACGGGTGA GGGCTTCATC GACTTCATCG GGCAGGTGCA CAAGACCAAG 
GAGCAGTTCG GGCAGGACGG GCCCATCACG GTGCACTGCA GTGCCGGCGT GGGCCGCACC 
GGGGTGTTCA TCACTCTGAG CATCGTCCTG GAGCGGATGC GCTACGAGGG CGTGGTCGAC 
ATGTTCCAGA CCGTGAAGAC CCTGCGCACA CAGCGCCCAG CCATGGTGCA GACGGAGGAC 
CAGTACCAGC TGTGCTATCG TGCGGCCCTG GAGTACCTTG GCAGCTTTGA CCACTATGCA 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629172.1
CDS	125..5590
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGGSPIRGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSMPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQWRPEESEDYETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGSSVPSCPNISSMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_005629115.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629115.3

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ATGCCCAGTG GACCGCCACG GAAGGTGGAG 
GTGGAACCAC TGAATTCCAC TGCTGTGCGT GTCTCTTGGA AGCTGCCCGT CCCCAGCAAG 
CAGCACGGCC AGATTCGCGG CTACCAGGTC ACCTATGTGC GGCTAGAGAA TGGCGAGCCC 
CGTGGCCCAC CCATCATCCA GGACGTCATG CTGGCTGAGG CCCAGTGGCG GCCAGAGGAG 
TCCGAGGACT ATGAAACCAT AATCAGCGGC CTGACCCCAG AGACCACCTA TTCCATTACC 
GTCGCCGCCT ACACCACCAA AGGGGATGGT GCCCGCAGCA AGCCCAAAGT TGTCACAACG 
ACCGGGGCAG TCCCAGGCCG GCCCACCATG ATGGTCAGCA CCACGGCCAT GAACACCGCA 
CTGCTGCAGT GGCACCCCCC CAAGGAGCTG CCAGGGGAGC TGCTGGGCTA CCGGCTACAG 
TACCACCGTG CCGATGAGGC AAAGCCCAAC ATCCTAGATT TTGGCAAGGA CGACCAGCAC 
TTTACAGTCA CCGGCCTACA CAAGGGAGCC ACCTACATTT TCCGGCTTGC CGCCAAGAAC 
CGGGCTGGCC CGGGAGAAGA GTTCGAGAAG GAGATCACGA CTCCCGAGGA TGCGCCCAGC 
GGCTTCCCCC AAAACCTGCG CGTGATAGGG CTGACCACCT CTACCACGGA GCTGACCTGG 
GACCCCCCGG TGCTGGCGGA GAGGAACGGG CGCATCACCA ACTACTCCGT GGTGTACCGT 
GACATCAACA GCCAGCAGGA GCTACAGAAC GTCACCGCCG ACACCCACCT CATGCTCTCC 
GGCCTCAAGC CGGACACCAC TTACGACATC AAGGTCCGCG CACGTACCAG CAAGGGCGCC 
GGCCCACTCA GCCCCAGCAT CCAGTCCCGG ACCATGCCCA TGGAGCAAGT GTTTGCCAAG 
AACTTCCGTG TGGCGGCCGC TATGAAGACG TCTGTGCTGC TCAGCTGGGA GGTCCCAGAC 
TCCTACAAGT CGGCTGTACC CTTCAAGATC CTGTACAACG GGCAGAGTGT GGAGGTGGAC 
GGGCACTCCA TGCGGAAACT GATCGCAGAC CTGCAGCCGA ACACGGAGTA CTCGTTCGTG 
CTGATGAACC GTGGCAGCAG CGCGGGGGGC CTGCAGCACC TCGTGTCCAT CCGCACGGCC 
CCAGACCTCC TGCCACACAA GCCGATGCCC GCCTCCGCCT TCATAGAGGA TGGTCGCTTC 
ACCCTCTCCA TGCCCCACGT GCAGGATGCC TCACTCATCA GGTGGTTCTA CATCGTGGTG 
GTACCCATCG ACCGCGTGGG TGGGAGTGTG CTGGCCCCGA GATGGAGCAC GCCTGAGGAG 
CTGGAGCTGG ACGAGCTTCT GGAGGCCATC GAGCAGGGCG GTGGGGAGCG GCGACGACGG 
CGGCAGGCGG AGCGGCTGAA GCCATATGTG GCTGCACAGG TGGACGTGCT CCCCGAGAGC 
TTCACCCTAG GAGACAAGAA GAGCTACCGG GGCTTCTACA ACCGGCCTCT GTCTCCGGAC 
CTGAGCTATC AGTGCTTTGT GCTCGCCTCC CTGAAGGAAC CCGTGGACCA GAAGCGCTAT 
GCCTCCAGCC CCTACTCTGA TGAGATCGTG GTCCAAGTGA CACCAGCCCA GCAGCAGGAG 
GAGCCTGAGA TGCTGTGGGT GACAGGCCCC GTCCTCGCAG TCATCCTCAT CATCCTCATT 
GTCATCGCCA TCCTCCTGTT CAAGAGGAAG AGGACTCACT CCCCATCATC CAAGGACGAG 
CAGTCAATCG GGCTGAAGGA CTCCTTGCTG GCCCATTCCT CTGACCCTGT GGAGATGCGG 
AGGCTCAACT ACCAGACTCC AGGTTCCAGT GTCCCCAGTT GCCCGAATAT CTCAAGTATG 
CGAGACCACC CGCCCATCCC CATCACTGAC CTGGCCGACA ACATTGAGCG CCTCAAAGCC 
AACGACGGCC TCAAGTTCTC CCAGGAGTAC GAGTCCATTG ACCCTGGACA GCAGTTCACC 
TGGGAGAATT CAAACCTGGA AGTGAACAAG CCCAAGAACC GCTATGCAAA CGTCATCGCC 
TACGACCACT CCCGAGTCAT CCTTACCTCC GTCGATGGTG TCCCAGGGAG TGACTATATC 
AACGCCAACT ACATCGATGG CTACCGCAAG CAGAACGCCT ACATTGCCAC GCAGGGCCCA 
CTGCCCGAGA CCATGGGCGA TTTCTGGCGG ATGGTGTGGG AACAGCGCAC AGCCACCGTG 
GTCATGATGA CACGGCTGGA GGAGAAGTCT CGGGTGAAGT GTGATCAGTA CTGGCCAGCC 
CGGGGTACCG AGACCCACGG CCTCATTCAG GTGACCCTGC TGGACACGGT GGAGCTGGCC 
ACATACACTG TGCGCACCTT TGCACTCCAC AAGAGTGGCT CCAGCGAGAA GCGGGAGCTG 
CGTCAGTTCC AGTTCATGGC CTGGCCGGAC CACGGGGTTC CCGAGTACCC CACCCCCATC 
CTGGCCTTCC TGCGGCGGGT CAAGGCCTGT AACCCGCTAG ACGCAGGGCC CATGGTGGTG 
CACTGCAGCG CGGGCGTGGG CCGCACGGGC TGTTTCATCG TCATCGACGC CATGCTGGAG 
CGGATGAAGC ACGAGAAGAC AGTGGACATC TATGGCCACG TGACATGCAT GCGATCGCAG 
CGGAACTACA TGGTGCAGAC AGAGGACCAG TACGTGTTCA TCCACGAGGC GCTGCTAGAG 
GCCGCCACGT GCGGACACAC GGAGGTGCCT GCCCGCAGCC TGTATGCCCA TATCCAGAAG 
CTGGGCCAAG TGCCTCCCGG GGAGAGTGTG ACTGCCATGG AGCTCGAGTT CAAGCTGCTG 
GCCAGCTCCA AGGCCCACAC ATCCCGCTTC ATTAGCGCCA ACCTGCCCTG CAACAAGTTC 
AAGAACCGCC TGGTGAACAT CATGCCCTAT GAGTTGACCC GTGTGTGTCT GCAGCCCATC 
CGTGGTGTGG AGGGCTCTGA CTACATCAAT GCCAGCTTCT TGGACGGCTA TAGGCAGCAG 
AAGGCCTACA TCGCCACGCA GGGGCCCCTG GCAGAGAGCA CCGAGGACTT CTGGCGCATG 
CTCTGGGAGC ACGGCTCCAC CATCGTCGTC ATGCTGACCA GGCTTCGGGA GATGGGCAGG 
GAGAAATGCC ACCAGTACTG GCCAGCAGAG CGCTCCGCTC GCTACCAATA CTTTGTCGTT 
GACCCGATGG CTGAGTACAA CATGCCGCAG TACATCCTGC GTGAGTTCAA GGTCACAGAT 
GCCCGGGATG GGCAGTCGAG GACAATCCGG CAGTTCCAGT TCACAGACTG GCCCGAGCAG 
GGCGTGCCTA AGACGGGTGA GGGCTTCATC GACTTCATCG GGCAGGTGCA CAAGACCAAG 
GAGCAGTTCG GGCAGGACGG GCCCATCACG GTGCACTGCA GTGCCGGCGT GGGCCGCACC 
GGGGTGTTCA TCACTCTGAG CATCGTCCTG GAGCGGATGC GCTACGAGGG CGTGGTCGAC 
ATGTTCCAGA CCGTGAAGAC CCTGCGCACA CAGCGCCCAG CCATGGTGCA GACGGAGGAC 
CAGTACCAGC TGTGCTATCG TGCGGCCCTG GAGTACCTTG GCAGCTTTGA CCACTATGCA 
ACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_005629116.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629173.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X8, mRNA.

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CloneID	OCe17427
Clone ID Related Accession (Same CDS sequence)	XM_005629116.2 , XM_005629116.3
Accession Version	XM_005629116.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629116.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCATGCCCAG TGGACCGCCA CGGAAGGTGG AGGTGGAACC ACTGAATTCC 
ACTGCTGTGC GTGTCTCTTG GAAGCTGCCC GTCCCCAGCA AGCAGCACGG CCAGATTCGC 
GGCTACCAGG TCACCTATGT GCGGCTAGAG AATGGCGAGC CCCGTGGCCC ACCCATCATC 
CAGGACGTCA TGCTGGCTGA GGCCCAGGAA ACCATAATCA GCGGCCTGAC CCCAGAGACC 
ACCTATTCCA TTACCGTCGC CGCCTACACC ACCAAAGGGG ATGGTGCCCG CAGCAAGCCC 
AAAGTTGTCA CAACGACCGG GGCAGTCCCA GGCCGGCCCA CCATGATGGT CAGCACCACG 
GCCATGAACA CCGCACTGCT GCAGTGGCAC CCCCCCAAGG AGCTGCCAGG GGAGCTGCTG 
GGCTACCGGC TACAGTACCA CCGTGCCGAT GAGGCAAAGC CCAACATCCT AGATTTTGGC 
AAGGACGACC AGCACTTTAC AGTCACCGGC CTACACAAGG GAGCCACCTA CATTTTCCGG 
CTTGCCGCCA AGAACCGGGC TGGCCCGGGA GAAGAGTTCG AGAAGGAGAT CACGACTCCC 
GAGGATGCGC CCAGCGGCTT CCCCCAAAAC CTGCGCGTGA TAGGGCTGAC CACCTCTACC 
ACGGAGCTGA CCTGGGACCC CCCGGTGCTG GCGGAGAGGA ACGGGCGCAT CACCAACTAC 
TCCGTGGTGT ACCGTGACAT CAACAGCCAG CAGGAGCTAC AGAACGTCAC CGCCGACACC 
CACCTCATGC TCTCCGGCCT CAAGCCGGAC ACCACTTACG ACATCAAGGT CCGCGCACGT 
ACCAGCAAGG GCGCCGGCCC ACTCAGCCCC AGCATCCAGT CCCGGACCAT GCCCATGGAG 
CAAGTGTTTG CCAAGAACTT CCGTGTGGCG GCCGCTATGA AGACGTCTGT GCTGCTCAGC 
TGGGAGGTCC CAGACTCCTA CAAGTCGGCT GTACCCTTCA AGATCCTGTA CAACGGGCAG 
AGTGTGGAGG TGGACGGGCA CTCCATGCGG AAACTGATCG CAGACCTGCA GCCGAACACG 
GAGTACTCGT TCGTGCTGAT GAACCGTGGC AGCAGCGCGG GGGGCCTGCA GCACCTCGTG 
TCCATCCGCA CGGCCCCAGA CCTCCTGCCA CACAAGCCGA TGCCCGCCTC CGCCTTCATA 
GAGGATGGTC GCTTCACCCT CTCCATGCCC CACGTGCAGG ATGCCTCACT CATCAGGTGG 
TTCTACATCG TGGTGGTACC CATCGACCGC GTGGGTGGGA GTGTGCTGGC CCCGAGATGG 
AGCACGCCTG AGGAGCTGGA GCTGGACGAG CTTCTGGAGG CCATCGAGCA GGGCGGTGGG 
GAGCGGCGAC GACGGCGGCA GGCGGAGCGG CTGAAGCCAT ATGTGGCTGC ACAGGTGGAC 
GTGCTCCCCG AGAGCTTCAC CCTAGGAGAC AAGAAGAGCT ACCGGGGCTT CTACAACCGG 
CCTCTGTCTC CGGACCTGAG CTATCAGTGC TTTGTGCTCG CCTCCCTGAA GGAACCCGTG 
GACCAGAAGC GCTATGCCTC CAGCCCCTAC TCTGATGAGA TCGTGGTCCA AGTGACACCA 
GCCCAGCAGC AGGAGGAGCC TGAGATGCTG TGGGTGACAG GCCCCGTCCT CGCAGTCATC 
CTCATCATCC TCATTGTCAT CGCCATCCTC CTGTTCAAGA GGAAGAGGAC TCACTCCCCA 
TCATCCAAGG ACGAGCAGTC AATCGGGCTG AAGGACTCCT TGCTGGCCCA TTCCTCTGAC 
CCTGTGGAGA TGCGGAGGCT CAACTACCAG ACTCCAGGTA TGCGAGACCA CCCGCCCATC 
CCCATCACTG ACCTGGCCGA CAACATTGAG CGCCTCAAAG CCAACGACGG CCTCAAGTTC 
TCCCAGGAGT ACGAGTCCAT TGACCCTGGA CAGCAGTTCA CCTGGGAGAA TTCAAACCTG 
GAAGTGAACA AGCCCAAGAA CCGCTATGCA AACGTCATCG CCTACGACCA CTCCCGAGTC 
ATCCTTACCT CCGTCGATGG TGTCCCAGGG AGTGACTATA TCAACGCCAA CTACATCGAT 
GGCTACCGCA AGCAGAACGC CTACATTGCC ACGCAGGGCC CACTGCCCGA GACCATGGGC 
GATTTCTGGC GGATGGTGTG GGAACAGCGC ACAGCCACCG TGGTCATGAT GACACGGCTG 
GAGGAGAAGT CTCGGGTGAA GTGTGATCAG TACTGGCCAG CCCGGGGTAC CGAGACCCAC 
GGCCTCATTC AGGTGACCCT GCTGGACACG GTGGAGCTGG CCACATACAC TGTGCGCACC 
TTTGCACTCC ACAAGAGTGG CTCCAGCGAG AAGCGGGAGC TGCGTCAGTT CCAGTTCATG 
GCCTGGCCGG ACCACGGGGT TCCCGAGTAC CCCACCCCCA TCCTGGCCTT CCTGCGGCGG 
GTCAAGGCCT GTAACCCGCT AGACGCAGGG CCCATGGTGG TGCACTGCAG CGCGGGCGTG 
GGCCGCACGG GCTGTTTCAT CGTCATCGAC GCCATGCTGG AGCGGATGAA GCACGAGAAG 
ACAGTGGACA TCTATGGCCA CGTGACATGC ATGCGATCGC AGCGGAACTA CATGGTGCAG 
ACAGAGGACC AGTACGTGTT CATCCACGAG GCGCTGCTAG AGGCCGCCAC GTGCGGACAC 
ACGGAGGTGC CTGCCCGCAG CCTGTATGCC CATATCCAGA AGCTGGGCCA AGTGCCTCCC 
GGGGAGAGTG TGACTGCCAT GGAGCTCGAG TTCAAGCTGC TGGCCAGCTC CAAGGCCCAC 
ACATCCCGCT TCATTAGCGC CAACCTGCCC TGCAACAAGT TCAAGAACCG CCTGGTGAAC 
ATCATGCCCT ATGAGTTGAC CCGTGTGTGT CTGCAGCCCA TCCGTGGTGT GGAGGGCTCT 
GACTACATCA ATGCCAGCTT CTTGGACGGC TATAGGCAGC AGAAGGCCTA CATCGCCACG 
CAGGGGCCCC TGGCAGAGAG CACCGAGGAC TTCTGGCGCA TGCTCTGGGA GCACGGCTCC 
ACCATCGTCG TCATGCTGAC CAGGCTTCGG GAGATGGGCA GGGAGAAATG CCACCAGTAC 
TGGCCAGCAG AGCGCTCCGC TCGCTACCAA TACTTTGTCG TTGACCCGAT GGCTGAGTAC 
AACATGCCGC AGTACATCCT GCGTGAGTTC AAGGTCACAG ATGCCCGGGA TGGGCAGTCG 
AGGACAATCC GGCAGTTCCA GTTCACAGAC TGGCCCGAGC AGGGCGTGCC TAAGACGGGT 
GAGGGCTTCA TCGACTTCAT CGGGCAGGTG CACAAGACCA AGGAGCAGTT CGGGCAGGAC 
GGGCCCATCA CGGTGCACTG CAGTGCCGGC GTGGGCCGCA CCGGGGTGTT CATCACTCTG 
AGCATCGTCC TGGAGCGGAT GCGCTACGAG GGCGTGGTCG ACATGTTCCA GACCGTGAAG 
ACCCTGCGCA CACAGCGCCC AGCCATGGTG CAGACGGAGG ACCAGTACCA GCTGTGCTAT 
CGTGCGGCCC TGGAGTACCT TGGCAGCTTT GACCACTATG CAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629173.1
CDS	125..5512
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSMPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_005629116.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629116.2

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCATGCCCAG TGGACCGCCA CGGAAGGTGG AGGTGGAACC ACTGAATTCC 
ACTGCTGTGC GTGTCTCTTG GAAGCTGCCC GTCCCCAGCA AGCAGCACGG CCAGATTCGC 
GGCTACCAGG TCACCTATGT GCGGCTAGAG AATGGCGAGC CCCGTGGCCC ACCCATCATC 
CAGGACGTCA TGCTGGCTGA GGCCCAGGAA ACCATAATCA GCGGCCTGAC CCCAGAGACC 
ACCTATTCCA TTACCGTCGC CGCCTACACC ACCAAAGGGG ATGGTGCCCG CAGCAAGCCC 
AAAGTTGTCA CAACGACCGG GGCAGTCCCA GGCCGGCCCA CCATGATGGT CAGCACCACG 
GCCATGAACA CCGCACTGCT GCAGTGGCAC CCCCCCAAGG AGCTGCCAGG GGAGCTGCTG 
GGCTACCGGC TACAGTACCA CCGTGCCGAT GAGGCAAAGC CCAACATCCT AGATTTTGGC 
AAGGACGACC AGCACTTTAC AGTCACCGGC CTACACAAGG GAGCCACCTA CATTTTCCGG 
CTTGCCGCCA AGAACCGGGC TGGCCCGGGA GAAGAGTTCG AGAAGGAGAT CACGACTCCC 
GAGGATGCGC CCAGCGGCTT CCCCCAAAAC CTGCGCGTGA TAGGGCTGAC CACCTCTACC 
ACGGAGCTGA CCTGGGACCC CCCGGTGCTG GCGGAGAGGA ACGGGCGCAT CACCAACTAC 
TCCGTGGTGT ACCGTGACAT CAACAGCCAG CAGGAGCTAC AGAACGTCAC CGCCGACACC 
CACCTCATGC TCTCCGGCCT CAAGCCGGAC ACCACTTACG ACATCAAGGT CCGCGCACGT 
ACCAGCAAGG GCGCCGGCCC ACTCAGCCCC AGCATCCAGT CCCGGACCAT GCCCATGGAG 
CAAGTGTTTG CCAAGAACTT CCGTGTGGCG GCCGCTATGA AGACGTCTGT GCTGCTCAGC 
TGGGAGGTCC CAGACTCCTA CAAGTCGGCT GTACCCTTCA AGATCCTGTA CAACGGGCAG 
AGTGTGGAGG TGGACGGGCA CTCCATGCGG AAACTGATCG CAGACCTGCA GCCGAACACG 
GAGTACTCGT TCGTGCTGAT GAACCGTGGC AGCAGCGCGG GGGGCCTGCA GCACCTCGTG 
TCCATCCGCA CGGCCCCAGA CCTCCTGCCA CACAAGCCGA TGCCCGCCTC CGCCTTCATA 
GAGGATGGTC GCTTCACCCT CTCCATGCCC CACGTGCAGG ATGCCTCACT CATCAGGTGG 
TTCTACATCG TGGTGGTACC CATCGACCGC GTGGGTGGGA GTGTGCTGGC CCCGAGATGG 
AGCACGCCTG AGGAGCTGGA GCTGGACGAG CTTCTGGAGG CCATCGAGCA GGGCGGTGGG 
GAGCGGCGAC GACGGCGGCA GGCGGAGCGG CTGAAGCCAT ATGTGGCTGC ACAGGTGGAC 
GTGCTCCCCG AGAGCTTCAC CCTAGGAGAC AAGAAGAGCT ACCGGGGCTT CTACAACCGG 
CCTCTGTCTC CGGACCTGAG CTATCAGTGC TTTGTGCTCG CCTCCCTGAA GGAACCCGTG 
GACCAGAAGC GCTATGCCTC CAGCCCCTAC TCTGATGAGA TCGTGGTCCA AGTGACACCA 
GCCCAGCAGC AGGAGGAGCC TGAGATGCTG TGGGTGACAG GCCCCGTCCT CGCAGTCATC 
CTCATCATCC TCATTGTCAT CGCCATCCTC CTGTTCAAGA GGAAGAGGAC TCACTCCCCA 
TCATCCAAGG ACGAGCAGTC AATCGGGCTG AAGGACTCCT TGCTGGCCCA TTCCTCTGAC 
CCTGTGGAGA TGCGGAGGCT CAACTACCAG ACTCCAGGTA TGCGAGACCA CCCGCCCATC 
CCCATCACTG ACCTGGCCGA CAACATTGAG CGCCTCAAAG CCAACGACGG CCTCAAGTTC 
TCCCAGGAGT ACGAGTCCAT TGACCCTGGA CAGCAGTTCA CCTGGGAGAA TTCAAACCTG 
GAAGTGAACA AGCCCAAGAA CCGCTATGCA AACGTCATCG CCTACGACCA CTCCCGAGTC 
ATCCTTACCT CCGTCGATGG TGTCCCAGGG AGTGACTATA TCAACGCCAA CTACATCGAT 
GGCTACCGCA AGCAGAACGC CTACATTGCC ACGCAGGGCC CACTGCCCGA GACCATGGGC 
GATTTCTGGC GGATGGTGTG GGAACAGCGC ACAGCCACCG TGGTCATGAT GACACGGCTG 
GAGGAGAAGT CTCGGGTGAA GTGTGATCAG TACTGGCCAG CCCGGGGTAC CGAGACCCAC 
GGCCTCATTC AGGTGACCCT GCTGGACACG GTGGAGCTGG CCACATACAC TGTGCGCACC 
TTTGCACTCC ACAAGAGTGG CTCCAGCGAG AAGCGGGAGC TGCGTCAGTT CCAGTTCATG 
GCCTGGCCGG ACCACGGGGT TCCCGAGTAC CCCACCCCCA TCCTGGCCTT CCTGCGGCGG 
GTCAAGGCCT GTAACCCGCT AGACGCAGGG CCCATGGTGG TGCACTGCAG CGCGGGCGTG 
GGCCGCACGG GCTGTTTCAT CGTCATCGAC GCCATGCTGG AGCGGATGAA GCACGAGAAG 
ACAGTGGACA TCTATGGCCA CGTGACATGC ATGCGATCGC AGCGGAACTA CATGGTGCAG 
ACAGAGGACC AGTACGTGTT CATCCACGAG GCGCTGCTAG AGGCCGCCAC GTGCGGACAC 
ACGGAGGTGC CTGCCCGCAG CCTGTATGCC CATATCCAGA AGCTGGGCCA AGTGCCTCCC 
GGGGAGAGTG TGACTGCCAT GGAGCTCGAG TTCAAGCTGC TGGCCAGCTC CAAGGCCCAC 
ACATCCCGCT TCATTAGCGC CAACCTGCCC TGCAACAAGT TCAAGAACCG CCTGGTGAAC 
ATCATGCCCT ATGAGTTGAC CCGTGTGTGT CTGCAGCCCA TCCGTGGTGT GGAGGGCTCT 
GACTACATCA ATGCCAGCTT CTTGGACGGC TATAGGCAGC AGAAGGCCTA CATCGCCACG 
CAGGGGCCCC TGGCAGAGAG CACCGAGGAC TTCTGGCGCA TGCTCTGGGA GCACGGCTCC 
ACCATCGTCG TCATGCTGAC CAGGCTTCGG GAGATGGGCA GGGAGAAATG CCACCAGTAC 
TGGCCAGCAG AGCGCTCCGC TCGCTACCAA TACTTTGTCG TTGACCCGAT GGCTGAGTAC 
AACATGCCGC AGTACATCCT GCGTGAGTTC AAGGTCACAG ATGCCCGGGA TGGGCAGTCG 
AGGACAATCC GGCAGTTCCA GTTCACAGAC TGGCCCGAGC AGGGCGTGCC TAAGACGGGT 
GAGGGCTTCA TCGACTTCAT CGGGCAGGTG CACAAGACCA AGGAGCAGTT CGGGCAGGAC 
GGGCCCATCA CGGTGCACTG CAGTGCCGGC GTGGGCCGCA CCGGGGTGTT CATCACTCTG 
AGCATCGTCC TGGAGCGGAT GCGCTACGAG GGCGTGGTCG ACATGTTCCA GACCGTGAAG 
ACCCTGCGCA CACAGCGCCC AGCCATGGTG CAGACGGAGG ACCAGTACCA GCTGTGCTAT 
CGTGCGGCCC TGGAGTACCT TGGCAGCTTT GACCACTATG CAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_005629116.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629173.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X9, mRNA.

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CloneID	OCe17427
Clone ID Related Accession (Same CDS sequence)	XM_005629116.2 , XM_005629116.3
Accession Version	XM_005629116.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629116.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCATGCCCAG TGGACCGCCA CGGAAGGTGG AGGTGGAACC ACTGAATTCC 
ACTGCTGTGC GTGTCTCTTG GAAGCTGCCC GTCCCCAGCA AGCAGCACGG CCAGATTCGC 
GGCTACCAGG TCACCTATGT GCGGCTAGAG AATGGCGAGC CCCGTGGCCC ACCCATCATC 
CAGGACGTCA TGCTGGCTGA GGCCCAGGAA ACCATAATCA GCGGCCTGAC CCCAGAGACC 
ACCTATTCCA TTACCGTCGC CGCCTACACC ACCAAAGGGG ATGGTGCCCG CAGCAAGCCC 
AAAGTTGTCA CAACGACCGG GGCAGTCCCA GGCCGGCCCA CCATGATGGT CAGCACCACG 
GCCATGAACA CCGCACTGCT GCAGTGGCAC CCCCCCAAGG AGCTGCCAGG GGAGCTGCTG 
GGCTACCGGC TACAGTACCA CCGTGCCGAT GAGGCAAAGC CCAACATCCT AGATTTTGGC 
AAGGACGACC AGCACTTTAC AGTCACCGGC CTACACAAGG GAGCCACCTA CATTTTCCGG 
CTTGCCGCCA AGAACCGGGC TGGCCCGGGA GAAGAGTTCG AGAAGGAGAT CACGACTCCC 
GAGGATGCGC CCAGCGGCTT CCCCCAAAAC CTGCGCGTGA TAGGGCTGAC CACCTCTACC 
ACGGAGCTGA CCTGGGACCC CCCGGTGCTG GCGGAGAGGA ACGGGCGCAT CACCAACTAC 
TCCGTGGTGT ACCGTGACAT CAACAGCCAG CAGGAGCTAC AGAACGTCAC CGCCGACACC 
CACCTCATGC TCTCCGGCCT CAAGCCGGAC ACCACTTACG ACATCAAGGT CCGCGCACGT 
ACCAGCAAGG GCGCCGGCCC ACTCAGCCCC AGCATCCAGT CCCGGACCAT GCCCATGGAG 
CAAGTGTTTG CCAAGAACTT CCGTGTGGCG GCCGCTATGA AGACGTCTGT GCTGCTCAGC 
TGGGAGGTCC CAGACTCCTA CAAGTCGGCT GTACCCTTCA AGATCCTGTA CAACGGGCAG 
AGTGTGGAGG TGGACGGGCA CTCCATGCGG AAACTGATCG CAGACCTGCA GCCGAACACG 
GAGTACTCGT TCGTGCTGAT GAACCGTGGC AGCAGCGCGG GGGGCCTGCA GCACCTCGTG 
TCCATCCGCA CGGCCCCAGA CCTCCTGCCA CACAAGCCGA TGCCCGCCTC CGCCTTCATA 
GAGGATGGTC GCTTCACCCT CTCCATGCCC CACGTGCAGG ATGCCTCACT CATCAGGTGG 
TTCTACATCG TGGTGGTACC CATCGACCGC GTGGGTGGGA GTGTGCTGGC CCCGAGATGG 
AGCACGCCTG AGGAGCTGGA GCTGGACGAG CTTCTGGAGG CCATCGAGCA GGGCGGTGGG 
GAGCGGCGAC GACGGCGGCA GGCGGAGCGG CTGAAGCCAT ATGTGGCTGC ACAGGTGGAC 
GTGCTCCCCG AGAGCTTCAC CCTAGGAGAC AAGAAGAGCT ACCGGGGCTT CTACAACCGG 
CCTCTGTCTC CGGACCTGAG CTATCAGTGC TTTGTGCTCG CCTCCCTGAA GGAACCCGTG 
GACCAGAAGC GCTATGCCTC CAGCCCCTAC TCTGATGAGA TCGTGGTCCA AGTGACACCA 
GCCCAGCAGC AGGAGGAGCC TGAGATGCTG TGGGTGACAG GCCCCGTCCT CGCAGTCATC 
CTCATCATCC TCATTGTCAT CGCCATCCTC CTGTTCAAGA GGAAGAGGAC TCACTCCCCA 
TCATCCAAGG ACGAGCAGTC AATCGGGCTG AAGGACTCCT TGCTGGCCCA TTCCTCTGAC 
CCTGTGGAGA TGCGGAGGCT CAACTACCAG ACTCCAGGTA TGCGAGACCA CCCGCCCATC 
CCCATCACTG ACCTGGCCGA CAACATTGAG CGCCTCAAAG CCAACGACGG CCTCAAGTTC 
TCCCAGGAGT ACGAGTCCAT TGACCCTGGA CAGCAGTTCA CCTGGGAGAA TTCAAACCTG 
GAAGTGAACA AGCCCAAGAA CCGCTATGCA AACGTCATCG CCTACGACCA CTCCCGAGTC 
ATCCTTACCT CCGTCGATGG TGTCCCAGGG AGTGACTATA TCAACGCCAA CTACATCGAT 
GGCTACCGCA AGCAGAACGC CTACATTGCC ACGCAGGGCC CACTGCCCGA GACCATGGGC 
GATTTCTGGC GGATGGTGTG GGAACAGCGC ACAGCCACCG TGGTCATGAT GACACGGCTG 
GAGGAGAAGT CTCGGGTGAA GTGTGATCAG TACTGGCCAG CCCGGGGTAC CGAGACCCAC 
GGCCTCATTC AGGTGACCCT GCTGGACACG GTGGAGCTGG CCACATACAC TGTGCGCACC 
TTTGCACTCC ACAAGAGTGG CTCCAGCGAG AAGCGGGAGC TGCGTCAGTT CCAGTTCATG 
GCCTGGCCGG ACCACGGGGT TCCCGAGTAC CCCACCCCCA TCCTGGCCTT CCTGCGGCGG 
GTCAAGGCCT GTAACCCGCT AGACGCAGGG CCCATGGTGG TGCACTGCAG CGCGGGCGTG 
GGCCGCACGG GCTGTTTCAT CGTCATCGAC GCCATGCTGG AGCGGATGAA GCACGAGAAG 
ACAGTGGACA TCTATGGCCA CGTGACATGC ATGCGATCGC AGCGGAACTA CATGGTGCAG 
ACAGAGGACC AGTACGTGTT CATCCACGAG GCGCTGCTAG AGGCCGCCAC GTGCGGACAC 
ACGGAGGTGC CTGCCCGCAG CCTGTATGCC CATATCCAGA AGCTGGGCCA AGTGCCTCCC 
GGGGAGAGTG TGACTGCCAT GGAGCTCGAG TTCAAGCTGC TGGCCAGCTC CAAGGCCCAC 
ACATCCCGCT TCATTAGCGC CAACCTGCCC TGCAACAAGT TCAAGAACCG CCTGGTGAAC 
ATCATGCCCT ATGAGTTGAC CCGTGTGTGT CTGCAGCCCA TCCGTGGTGT GGAGGGCTCT 
GACTACATCA ATGCCAGCTT CTTGGACGGC TATAGGCAGC AGAAGGCCTA CATCGCCACG 
CAGGGGCCCC TGGCAGAGAG CACCGAGGAC TTCTGGCGCA TGCTCTGGGA GCACGGCTCC 
ACCATCGTCG TCATGCTGAC CAGGCTTCGG GAGATGGGCA GGGAGAAATG CCACCAGTAC 
TGGCCAGCAG AGCGCTCCGC TCGCTACCAA TACTTTGTCG TTGACCCGAT GGCTGAGTAC 
AACATGCCGC AGTACATCCT GCGTGAGTTC AAGGTCACAG ATGCCCGGGA TGGGCAGTCG 
AGGACAATCC GGCAGTTCCA GTTCACAGAC TGGCCCGAGC AGGGCGTGCC TAAGACGGGT 
GAGGGCTTCA TCGACTTCAT CGGGCAGGTG CACAAGACCA AGGAGCAGTT CGGGCAGGAC 
GGGCCCATCA CGGTGCACTG CAGTGCCGGC GTGGGCCGCA CCGGGGTGTT CATCACTCTG 
AGCATCGTCC TGGAGCGGAT GCGCTACGAG GGCGTGGTCG ACATGTTCCA GACCGTGAAG 
ACCCTGCGCA CACAGCGCCC AGCCATGGTG CAGACGGAGG ACCAGTACCA GCTGTGCTAT 
CGTGCGGCCC TGGAGTACCT TGGCAGCTTT GACCACTATG CAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629173.1
CDS	125..5512
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSMPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMNTALLQWHPPKELPGELLGYRLQYHRADEAKPNILDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFPQNLRVIGLTTSTTELTWDPPVLAERNGRITNYSVVYRDINSQQELQNVTADTHLMLSGLKPDTTYDIKVRARTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_005629116.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629116.3

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GCCTTGCAGA TTGAGAGCAG TGAGGAGTCG 
GACCAAGGCA AGTACGAGTG TGTGGCGACC AACTCCGCAG GCACACGCTA CTCGGCCCCT 
GCCAACCTAT ATGTACGAGT GCGCCGTGTG GCTCCTCGTT TCTCCATCCC TCCCAGCAGC 
CAGGAGGTGA TGCCGGGTGG CAGCGTGAAC CTGACTTGTG TTGCGGTGGG GGCACCCATG 
CCCTACGTGA AGTGGATGAT GGGGGCCGAG GAGCTCACCA AGGAGGATGA GATGCCAGTT 
GGCCGCAACG TGCTGGAGCT CAGCAATGTC GTGCGCTCTG CCAACTACAC CTGCGTGGCC 
ATCTCCTCTC TAGGCATGAT CGAGGCCACG GCCCAGGTCA CAGTGAAAGC TCTGCCGAAA 
CCTCCAATCG ACCTCCTGGT GACTGAGACA ACTGCCACCA GCGTCACCCT GACCTGGGAC 
TCTGGGAACT CAGAGCCTGT GTCCTACTAC GGCATCCAGT ACCGCGCGGC GGGGACGGAG 
GGCCCCTTCC AGGAGGTGGA CGGTGTGGCC ACCACCCGCT ACAGCATTGG CGGCCTCAGC 
CCCTTCTCCG AGTACGCCTT CCGCGTGCTG GCGGTGAACA GCATCGGCCG GGGACCACCC 
AGCGAGGCGG TGCGGGCACG CACGGGAGAG CAGGCGCCCT CCAGCCCCCC GCGCCGCGTG 
CAGGCACGCA TGCTGAGCGC GAGCACCATG CTGGTGCAGT GGGAGCCGCC CGAGGAGCCC 
AACGGCCTGG TGCGAGGGTA CCGCGTCTAC TACACTCCCG ACGCCCGCCG CCCCCTGAGC 
GCCTGGCACA AGCACAACAC GGACGCGGGC CTCCTCACCA CCGTGGGCAG CCTGCTGCCC 
GGAATCACCT ACAGCCTGCG CGTGCTGGCC TTCACCGCCG TGGGCGACGG CCCTCCCAGC 
CCCACCATCC AGGTCAAGAC ACAGCAGGGA GTGCCTGCGC AACCGGCGGA CTTCCAGGCT 
GAAGCAGAGT CGGACACCAG GATCCAGCTC TCGTGGCTGC TTCCCCCGCA GGAGCGGATC 
ATCAAGTACG AGCTGGTGTA TTGGGCAGCA GAGGATGAAG GCCAGCAGCA CAAGGTGACC 
TTCGACCCCA CCTCCTCCTA CACCCTGGAG GACCTGAAGC CTGACACACT GTACCACTTC 
CAGCTGGCCG CCCGTTCCGA GATGGGGGTG GGCGTGTTCA CCCCCACCGT TGAGGCCCGC 
ACAGCACAGT CCATGCCCAG TGGACCGCCA CGGAAGGTGG AGGTGGAACC ACTGAATTCC 
ACTGCTGTGC GTGTCTCTTG GAAGCTGCCC GTCCCCAGCA AGCAGCACGG CCAGATTCGC 
GGCTACCAGG TCACCTATGT GCGGCTAGAG AATGGCGAGC CCCGTGGCCC ACCCATCATC 
CAGGACGTCA TGCTGGCTGA GGCCCAGGAA ACCATAATCA GCGGCCTGAC CCCAGAGACC 
ACCTATTCCA TTACCGTCGC CGCCTACACC ACCAAAGGGG ATGGTGCCCG CAGCAAGCCC 
AAAGTTGTCA CAACGACCGG GGCAGTCCCA GGCCGGCCCA CCATGATGGT CAGCACCACG 
GCCATGAACA CCGCACTGCT GCAGTGGCAC CCCCCCAAGG AGCTGCCAGG GGAGCTGCTG 
GGCTACCGGC TACAGTACCA CCGTGCCGAT GAGGCAAAGC CCAACATCCT AGATTTTGGC 
AAGGACGACC AGCACTTTAC AGTCACCGGC CTACACAAGG GAGCCACCTA CATTTTCCGG 
CTTGCCGCCA AGAACCGGGC TGGCCCGGGA GAAGAGTTCG AGAAGGAGAT CACGACTCCC 
GAGGATGCGC CCAGCGGCTT CCCCCAAAAC CTGCGCGTGA TAGGGCTGAC CACCTCTACC 
ACGGAGCTGA CCTGGGACCC CCCGGTGCTG GCGGAGAGGA ACGGGCGCAT CACCAACTAC 
TCCGTGGTGT ACCGTGACAT CAACAGCCAG CAGGAGCTAC AGAACGTCAC CGCCGACACC 
CACCTCATGC TCTCCGGCCT CAAGCCGGAC ACCACTTACG ACATCAAGGT CCGCGCACGT 
ACCAGCAAGG GCGCCGGCCC ACTCAGCCCC AGCATCCAGT CCCGGACCAT GCCCATGGAG 
CAAGTGTTTG CCAAGAACTT CCGTGTGGCG GCCGCTATGA AGACGTCTGT GCTGCTCAGC 
TGGGAGGTCC CAGACTCCTA CAAGTCGGCT GTACCCTTCA AGATCCTGTA CAACGGGCAG 
AGTGTGGAGG TGGACGGGCA CTCCATGCGG AAACTGATCG CAGACCTGCA GCCGAACACG 
GAGTACTCGT TCGTGCTGAT GAACCGTGGC AGCAGCGCGG GGGGCCTGCA GCACCTCGTG 
TCCATCCGCA CGGCCCCAGA CCTCCTGCCA CACAAGCCGA TGCCCGCCTC CGCCTTCATA 
GAGGATGGTC GCTTCACCCT CTCCATGCCC CACGTGCAGG ATGCCTCACT CATCAGGTGG 
TTCTACATCG TGGTGGTACC CATCGACCGC GTGGGTGGGA GTGTGCTGGC CCCGAGATGG 
AGCACGCCTG AGGAGCTGGA GCTGGACGAG CTTCTGGAGG CCATCGAGCA GGGCGGTGGG 
GAGCGGCGAC GACGGCGGCA GGCGGAGCGG CTGAAGCCAT ATGTGGCTGC ACAGGTGGAC 
GTGCTCCCCG AGAGCTTCAC CCTAGGAGAC AAGAAGAGCT ACCGGGGCTT CTACAACCGG 
CCTCTGTCTC CGGACCTGAG CTATCAGTGC TTTGTGCTCG CCTCCCTGAA GGAACCCGTG 
GACCAGAAGC GCTATGCCTC CAGCCCCTAC TCTGATGAGA TCGTGGTCCA AGTGACACCA 
GCCCAGCAGC AGGAGGAGCC TGAGATGCTG TGGGTGACAG GCCCCGTCCT CGCAGTCATC 
CTCATCATCC TCATTGTCAT CGCCATCCTC CTGTTCAAGA GGAAGAGGAC TCACTCCCCA 
TCATCCAAGG ACGAGCAGTC AATCGGGCTG AAGGACTCCT TGCTGGCCCA TTCCTCTGAC 
CCTGTGGAGA TGCGGAGGCT CAACTACCAG ACTCCAGGTA TGCGAGACCA CCCGCCCATC 
CCCATCACTG ACCTGGCCGA CAACATTGAG CGCCTCAAAG CCAACGACGG CCTCAAGTTC 
TCCCAGGAGT ACGAGTCCAT TGACCCTGGA CAGCAGTTCA CCTGGGAGAA TTCAAACCTG 
GAAGTGAACA AGCCCAAGAA CCGCTATGCA AACGTCATCG CCTACGACCA CTCCCGAGTC 
ATCCTTACCT CCGTCGATGG TGTCCCAGGG AGTGACTATA TCAACGCCAA CTACATCGAT 
GGCTACCGCA AGCAGAACGC CTACATTGCC ACGCAGGGCC CACTGCCCGA GACCATGGGC 
GATTTCTGGC GGATGGTGTG GGAACAGCGC ACAGCCACCG TGGTCATGAT GACACGGCTG 
GAGGAGAAGT CTCGGGTGAA GTGTGATCAG TACTGGCCAG CCCGGGGTAC CGAGACCCAC 
GGCCTCATTC AGGTGACCCT GCTGGACACG GTGGAGCTGG CCACATACAC TGTGCGCACC 
TTTGCACTCC ACAAGAGTGG CTCCAGCGAG AAGCGGGAGC TGCGTCAGTT CCAGTTCATG 
GCCTGGCCGG ACCACGGGGT TCCCGAGTAC CCCACCCCCA TCCTGGCCTT CCTGCGGCGG 
GTCAAGGCCT GTAACCCGCT AGACGCAGGG CCCATGGTGG TGCACTGCAG CGCGGGCGTG 
GGCCGCACGG GCTGTTTCAT CGTCATCGAC GCCATGCTGG AGCGGATGAA GCACGAGAAG 
ACAGTGGACA TCTATGGCCA CGTGACATGC ATGCGATCGC AGCGGAACTA CATGGTGCAG 
ACAGAGGACC AGTACGTGTT CATCCACGAG GCGCTGCTAG AGGCCGCCAC GTGCGGACAC 
ACGGAGGTGC CTGCCCGCAG CCTGTATGCC CATATCCAGA AGCTGGGCCA AGTGCCTCCC 
GGGGAGAGTG TGACTGCCAT GGAGCTCGAG TTCAAGCTGC TGGCCAGCTC CAAGGCCCAC 
ACATCCCGCT TCATTAGCGC CAACCTGCCC TGCAACAAGT TCAAGAACCG CCTGGTGAAC 
ATCATGCCCT ATGAGTTGAC CCGTGTGTGT CTGCAGCCCA TCCGTGGTGT GGAGGGCTCT 
GACTACATCA ATGCCAGCTT CTTGGACGGC TATAGGCAGC AGAAGGCCTA CATCGCCACG 
CAGGGGCCCC TGGCAGAGAG CACCGAGGAC TTCTGGCGCA TGCTCTGGGA GCACGGCTCC 
ACCATCGTCG TCATGCTGAC CAGGCTTCGG GAGATGGGCA GGGAGAAATG CCACCAGTAC 
TGGCCAGCAG AGCGCTCCGC TCGCTACCAA TACTTTGTCG TTGACCCGAT GGCTGAGTAC 
AACATGCCGC AGTACATCCT GCGTGAGTTC AAGGTCACAG ATGCCCGGGA TGGGCAGTCG 
AGGACAATCC GGCAGTTCCA GTTCACAGAC TGGCCCGAGC AGGGCGTGCC TAAGACGGGT 
GAGGGCTTCA TCGACTTCAT CGGGCAGGTG CACAAGACCA AGGAGCAGTT CGGGCAGGAC 
GGGCCCATCA CGGTGCACTG CAGTGCCGGC GTGGGCCGCA CCGGGGTGTT CATCACTCTG 
AGCATCGTCC TGGAGCGGAT GCGCTACGAG GGCGTGGTCG ACATGTTCCA GACCGTGAAG 
ACCCTGCGCA CACAGCGCCC AGCCATGGTG CAGACGGAGG ACCAGTACCA GCTGTGCTAT 
CGTGCGGCCC TGGAGTACCT TGGCAGCTTT GACCACTATG CAACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_005629117.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629174.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X9, mRNA.

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CloneID	OCe17428
Clone ID Related Accession (Same CDS sequence)	XM_005629117.2 , XM_005629117.3
Accession Version	XM_005629117.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726085.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005629117.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GTGGCGGCCA 
GAGGAGTCCG AGGACTATGA AACCATAATC AGCGGCCTGA CCCCAGAGAC CACCTATTCC 
ATTACCGTCG CCGCCTACAC CACCAAAGGG GATGGTGCCC GCAGCAAGCC CAAAGTTGTC 
ACAACGACCG GGGCAGTGTT TGCCAAGAAC TTCCGTGTGG CGGCCGCTAT GAAGACGTCT 
GTGCTGCTCA GCTGGGAGGT CCCAGACTCC TACAAGTCGG CTGTACCCTT CAAGATCCTG 
TACAACGGGC AGAGTGTGGA GGTGGACGGG CACTCCATGC GGAAACTGAT CGCAGACCTG 
CAGCCGAACA CGGAGTACTC GTTCGTGCTG ATGAACCGTG GCAGCAGCGC GGGGGGCCTG 
CAGCACCTCG TGTCCATCCG CACGGCCCCA GACCTCCTGC CACACAAGCC GATGCCCGCC 
TCCGCCTTCA TAGAGGATGG TCGCTTCACC CTCTCCATGC CCCACGTGCA GGATGCCTCA 
CTCATCAGGT GGTTCTACAT CGTGGTGGTA CCCATCGACC GCGTGGGTGG GAGTGTGCTG 
GCCCCGAGAT GGAGCACGCC TGAGGAGCTG GAGCTGGACG AGCTTCTGGA GGCCATCGAG 
CAGGGCGGTG GGGAGCGGCG ACGACGGCGG CAGGCGGAGC GGCTGAAGCC ATATGTGGCT 
GCACAGGTGG ACGTGCTCCC CGAGAGCTTC ACCCTAGGAG ACAAGAAGAG CTACCGGGGC 
TTCTACAACC GGCCTCTGTC TCCGGACCTG AGCTATCAGT GCTTTGTGCT CGCCTCCCTG 
AAGGAACCCG TGGACCAGAA GCGCTATGCC TCCAGCCCCT ACTCTGATGA GATCGTGGTC 
CAAGTGACAC CAGCCCAGCA GCAGGAGGAG CCTGAGATGC TGTGGGTGAC AGGCCCCGTC 
CTCGCAGTCA TCCTCATCAT CCTCATTGTC ATCGCCATCC TCCTGTTCAA GAGGAAGAGG 
ACTCACTCCC CATCATCCAA GGACGAGCAG TCAATCGGGC TGAAGGACTC CTTGCTGGCC 
CATTCCTCTG ACCCTGTGGA GATGCGGAGG CTCAACTACC AGACTCCAGG TTCCAGTGTC 
CCCAGTTGCC CGAATATCTC AAGTATGCGA GACCACCCGC CCATCCCCAT CACTGACCTG 
GCCGACAACA TTGAGCGCCT CAAAGCCAAC GACGGCCTCA AGTTCTCCCA GGAGTACGAG 
TCCATTGACC CTGGACAGCA GTTCACCTGG GAGAATTCAA ACCTGGAAGT GAACAAGCCC 
AAGAACCGCT ATGCAAACGT CATCGCCTAC GACCACTCCC GAGTCATCCT TACCTCCGTC 
GATGGTGTCC CAGGGAGTGA CTATATCAAC GCCAACTACA TCGATGGCTA CCGCAAGCAG 
AACGCCTACA TTGCCACGCA GGGCCCACTG CCCGAGACCA TGGGCGATTT CTGGCGGATG 
GTGTGGGAAC AGCGCACAGC CACCGTGGTC ATGATGACAC GGCTGGAGGA GAAGTCTCGG 
GTGAAGTGTG ATCAGTACTG GCCAGCCCGG GGTACCGAGA CCCACGGCCT CATTCAGGTG 
ACCCTGCTGG ACACGGTGGA GCTGGCCACA TACACTGTGC GCACCTTTGC ACTCCACAAG 
AGTGGCTCCA GCGAGAAGCG GGAGCTGCGT CAGTTCCAGT TCATGGCCTG GCCGGACCAC 
GGGGTTCCCG AGTACCCCAC CCCCATCCTG GCCTTCCTGC GGCGGGTCAA GGCCTGTAAC 
CCGCTAGACG CAGGGCCCAT GGTGGTGCAC TGCAGCGCGG GCGTGGGCCG CACGGGCTGT 
TTCATCGTCA TCGACGCCAT GCTGGAGCGG ATGAAGCACG AGAAGACAGT GGACATCTAT 
GGCCACGTGA CATGCATGCG ATCGCAGCGG AACTACATGG TGCAGACAGA GGACCAGTAC 
GTGTTCATCC ACGAGGCGCT GCTAGAGGCC GCCACGTGCG GACACACGGA GGTGCCTGCC 
CGCAGCCTGT ATGCCCATAT CCAGAAGCTG GGCCAAGTGC CTCCCGGGGA GAGTGTGACT 
GCCATGGAGC TCGAGTTCAA GCTGCTGGCC AGCTCCAAGG CCCACACATC CCGCTTCATT 
AGCGCCAACC TGCCCTGCAA CAAGTTCAAG AACCGCCTGG TGAACATCAT GCCCTATGAG 
TTGACCCGTG TGTGTCTGCA GCCCATCCGT GGTGTGGAGG GCTCTGACTA CATCAATGCC 
AGCTTCTTGG ACGGCTATAG GCAGCAGAAG GCCTACATCG CCACGCAGGG GCCCCTGGCA 
GAGAGCACCG AGGACTTCTG GCGCATGCTC TGGGAGCACG GCTCCACCAT CGTCGTCATG 
CTGACCAGGC TTCGGGAGAT GGGCAGGGAG AAATGCCACC AGTACTGGCC AGCAGAGCGC 
TCCGCTCGCT ACCAATACTT TGTCGTTGAC CCGATGGCTG AGTACAACAT GCCGCAGTAC 
ATCCTGCGTG AGTTCAAGGT CACAGATGCC CGGGATGGGC AGTCGAGGAC AATCCGGCAG 
TTCCAGTTCA CAGACTGGCC CGAGCAGGGC GTGCCTAAGA CGGGTGAGGG CTTCATCGAC 
TTCATCGGGC AGGTGCACAA GACCAAGGAG CAGTTCGGGC AGGACGGGCC CATCACGGTG 
CACTGCAGTG CCGGCGTGGG CCGCACCGGG GTGTTCATCA CTCTGAGCAT CGTCCTGGAG 
CGGATGCGCT ACGAGGGCGT GGTCGACATG TTCCAGACCG TGAAGACCCT GCGCACACAG 
CGCCCAGCCA TGGTGCAGAC GGAGGACCAG TACCAGCTGT GCTATCGTGC GGCCCTGGAG 
TACCTTGGCA GCTTTGACCA CTATGCAACG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629174.1
CDS	125..5317
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGGSPIRGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPAASRNGVITQYSVAYEAVDGEDRGRHVVESISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQWRPEESEDYETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGSSVPSCPNISSMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_005629117.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type, F (PTPRF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629117.2

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GTGGCGGCCA 
GAGGAGTCCG AGGACTATGA AACCATAATC AGCGGCCTGA CCCCAGAGAC CACCTATTCC 
ATTACCGTCG CCGCCTACAC CACCAAAGGG GATGGTGCCC GCAGCAAGCC CAAAGTTGTC 
ACAACGACCG GGGCAGTGTT TGCCAAGAAC TTCCGTGTGG CGGCCGCTAT GAAGACGTCT 
GTGCTGCTCA GCTGGGAGGT CCCAGACTCC TACAAGTCGG CTGTACCCTT CAAGATCCTG 
TACAACGGGC AGAGTGTGGA GGTGGACGGG CACTCCATGC GGAAACTGAT CGCAGACCTG 
CAGCCGAACA CGGAGTACTC GTTCGTGCTG ATGAACCGTG GCAGCAGCGC GGGGGGCCTG 
CAGCACCTCG TGTCCATCCG CACGGCCCCA GACCTCCTGC CACACAAGCC GATGCCCGCC 
TCCGCCTTCA TAGAGGATGG TCGCTTCACC CTCTCCATGC CCCACGTGCA GGATGCCTCA 
CTCATCAGGT GGTTCTACAT CGTGGTGGTA CCCATCGACC GCGTGGGTGG GAGTGTGCTG 
GCCCCGAGAT GGAGCACGCC TGAGGAGCTG GAGCTGGACG AGCTTCTGGA GGCCATCGAG 
CAGGGCGGTG GGGAGCGGCG ACGACGGCGG CAGGCGGAGC GGCTGAAGCC ATATGTGGCT 
GCACAGGTGG ACGTGCTCCC CGAGAGCTTC ACCCTAGGAG ACAAGAAGAG CTACCGGGGC 
TTCTACAACC GGCCTCTGTC TCCGGACCTG AGCTATCAGT GCTTTGTGCT CGCCTCCCTG 
AAGGAACCCG TGGACCAGAA GCGCTATGCC TCCAGCCCCT ACTCTGATGA GATCGTGGTC 
CAAGTGACAC CAGCCCAGCA GCAGGAGGAG CCTGAGATGC TGTGGGTGAC AGGCCCCGTC 
CTCGCAGTCA TCCTCATCAT CCTCATTGTC ATCGCCATCC TCCTGTTCAA GAGGAAGAGG 
ACTCACTCCC CATCATCCAA GGACGAGCAG TCAATCGGGC TGAAGGACTC CTTGCTGGCC 
CATTCCTCTG ACCCTGTGGA GATGCGGAGG CTCAACTACC AGACTCCAGG TTCCAGTGTC 
CCCAGTTGCC CGAATATCTC AAGTATGCGA GACCACCCGC CCATCCCCAT CACTGACCTG 
GCCGACAACA TTGAGCGCCT CAAAGCCAAC GACGGCCTCA AGTTCTCCCA GGAGTACGAG 
TCCATTGACC CTGGACAGCA GTTCACCTGG GAGAATTCAA ACCTGGAAGT GAACAAGCCC 
AAGAACCGCT ATGCAAACGT CATCGCCTAC GACCACTCCC GAGTCATCCT TACCTCCGTC 
GATGGTGTCC CAGGGAGTGA CTATATCAAC GCCAACTACA TCGATGGCTA CCGCAAGCAG 
AACGCCTACA TTGCCACGCA GGGCCCACTG CCCGAGACCA TGGGCGATTT CTGGCGGATG 
GTGTGGGAAC AGCGCACAGC CACCGTGGTC ATGATGACAC GGCTGGAGGA GAAGTCTCGG 
GTGAAGTGTG ATCAGTACTG GCCAGCCCGG GGTACCGAGA CCCACGGCCT CATTCAGGTG 
ACCCTGCTGG ACACGGTGGA GCTGGCCACA TACACTGTGC GCACCTTTGC ACTCCACAAG 
AGTGGCTCCA GCGAGAAGCG GGAGCTGCGT CAGTTCCAGT TCATGGCCTG GCCGGACCAC 
GGGGTTCCCG AGTACCCCAC CCCCATCCTG GCCTTCCTGC GGCGGGTCAA GGCCTGTAAC 
CCGCTAGACG CAGGGCCCAT GGTGGTGCAC TGCAGCGCGG GCGTGGGCCG CACGGGCTGT 
TTCATCGTCA TCGACGCCAT GCTGGAGCGG ATGAAGCACG AGAAGACAGT GGACATCTAT 
GGCCACGTGA CATGCATGCG ATCGCAGCGG AACTACATGG TGCAGACAGA GGACCAGTAC 
GTGTTCATCC ACGAGGCGCT GCTAGAGGCC GCCACGTGCG GACACACGGA GGTGCCTGCC 
CGCAGCCTGT ATGCCCATAT CCAGAAGCTG GGCCAAGTGC CTCCCGGGGA GAGTGTGACT 
GCCATGGAGC TCGAGTTCAA GCTGCTGGCC AGCTCCAAGG CCCACACATC CCGCTTCATT 
AGCGCCAACC TGCCCTGCAA CAAGTTCAAG AACCGCCTGG TGAACATCAT GCCCTATGAG 
TTGACCCGTG TGTGTCTGCA GCCCATCCGT GGTGTGGAGG GCTCTGACTA CATCAATGCC 
AGCTTCTTGG ACGGCTATAG GCAGCAGAAG GCCTACATCG CCACGCAGGG GCCCCTGGCA 
GAGAGCACCG AGGACTTCTG GCGCATGCTC TGGGAGCACG GCTCCACCAT CGTCGTCATG 
CTGACCAGGC TTCGGGAGAT GGGCAGGGAG AAATGCCACC AGTACTGGCC AGCAGAGCGC 
TCCGCTCGCT ACCAATACTT TGTCGTTGAC CCGATGGCTG AGTACAACAT GCCGCAGTAC 
ATCCTGCGTG AGTTCAAGGT CACAGATGCC CGGGATGGGC AGTCGAGGAC AATCCGGCAG 
TTCCAGTTCA CAGACTGGCC CGAGCAGGGC GTGCCTAAGA CGGGTGAGGG CTTCATCGAC 
TTCATCGGGC AGGTGCACAA GACCAAGGAG CAGTTCGGGC AGGACGGGCC CATCACGGTG 
CACTGCAGTG CCGGCGTGGG CCGCACCGGG GTGTTCATCA CTCTGAGCAT CGTCCTGGAG 
CGGATGCGCT ACGAGGGCGT GGTCGACATG TTCCAGACCG TGAAGACCCT GCGCACACAG 
CGCCCAGCCA TGGTGCAGAC GGAGGACCAG TACCAGCTGT GCTATCGTGC GGCCCTGGAG 
TACCTTGGCA GCTTTGACCA CTATGCAACG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTPRF ( XM_005629117.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005629174.1 Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X10, mRNA.

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CloneID	OCe17428
Clone ID Related Accession (Same CDS sequence)	XM_005629117.2 , XM_005629117.3
Accession Version	XM_005629117.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	receptor-type tyrosine-protein phosphatase F isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006597.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629117.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GTGGCGGCCA 
GAGGAGTCCG AGGACTATGA AACCATAATC AGCGGCCTGA CCCCAGAGAC CACCTATTCC 
ATTACCGTCG CCGCCTACAC CACCAAAGGG GATGGTGCCC GCAGCAAGCC CAAAGTTGTC 
ACAACGACCG GGGCAGTGTT TGCCAAGAAC TTCCGTGTGG CGGCCGCTAT GAAGACGTCT 
GTGCTGCTCA GCTGGGAGGT CCCAGACTCC TACAAGTCGG CTGTACCCTT CAAGATCCTG 
TACAACGGGC AGAGTGTGGA GGTGGACGGG CACTCCATGC GGAAACTGAT CGCAGACCTG 
CAGCCGAACA CGGAGTACTC GTTCGTGCTG ATGAACCGTG GCAGCAGCGC GGGGGGCCTG 
CAGCACCTCG TGTCCATCCG CACGGCCCCA GACCTCCTGC CACACAAGCC GATGCCCGCC 
TCCGCCTTCA TAGAGGATGG TCGCTTCACC CTCTCCATGC CCCACGTGCA GGATGCCTCA 
CTCATCAGGT GGTTCTACAT CGTGGTGGTA CCCATCGACC GCGTGGGTGG GAGTGTGCTG 
GCCCCGAGAT GGAGCACGCC TGAGGAGCTG GAGCTGGACG AGCTTCTGGA GGCCATCGAG 
CAGGGCGGTG GGGAGCGGCG ACGACGGCGG CAGGCGGAGC GGCTGAAGCC ATATGTGGCT 
GCACAGGTGG ACGTGCTCCC CGAGAGCTTC ACCCTAGGAG ACAAGAAGAG CTACCGGGGC 
TTCTACAACC GGCCTCTGTC TCCGGACCTG AGCTATCAGT GCTTTGTGCT CGCCTCCCTG 
AAGGAACCCG TGGACCAGAA GCGCTATGCC TCCAGCCCCT ACTCTGATGA GATCGTGGTC 
CAAGTGACAC CAGCCCAGCA GCAGGAGGAG CCTGAGATGC TGTGGGTGAC AGGCCCCGTC 
CTCGCAGTCA TCCTCATCAT CCTCATTGTC ATCGCCATCC TCCTGTTCAA GAGGAAGAGG 
ACTCACTCCC CATCATCCAA GGACGAGCAG TCAATCGGGC TGAAGGACTC CTTGCTGGCC 
CATTCCTCTG ACCCTGTGGA GATGCGGAGG CTCAACTACC AGACTCCAGG TTCCAGTGTC 
CCCAGTTGCC CGAATATCTC AAGTATGCGA GACCACCCGC CCATCCCCAT CACTGACCTG 
GCCGACAACA TTGAGCGCCT CAAAGCCAAC GACGGCCTCA AGTTCTCCCA GGAGTACGAG 
TCCATTGACC CTGGACAGCA GTTCACCTGG GAGAATTCAA ACCTGGAAGT GAACAAGCCC 
AAGAACCGCT ATGCAAACGT CATCGCCTAC GACCACTCCC GAGTCATCCT TACCTCCGTC 
GATGGTGTCC CAGGGAGTGA CTATATCAAC GCCAACTACA TCGATGGCTA CCGCAAGCAG 
AACGCCTACA TTGCCACGCA GGGCCCACTG CCCGAGACCA TGGGCGATTT CTGGCGGATG 
GTGTGGGAAC AGCGCACAGC CACCGTGGTC ATGATGACAC GGCTGGAGGA GAAGTCTCGG 
GTGAAGTGTG ATCAGTACTG GCCAGCCCGG GGTACCGAGA CCCACGGCCT CATTCAGGTG 
ACCCTGCTGG ACACGGTGGA GCTGGCCACA TACACTGTGC GCACCTTTGC ACTCCACAAG 
AGTGGCTCCA GCGAGAAGCG GGAGCTGCGT CAGTTCCAGT TCATGGCCTG GCCGGACCAC 
GGGGTTCCCG AGTACCCCAC CCCCATCCTG GCCTTCCTGC GGCGGGTCAA GGCCTGTAAC 
CCGCTAGACG CAGGGCCCAT GGTGGTGCAC TGCAGCGCGG GCGTGGGCCG CACGGGCTGT 
TTCATCGTCA TCGACGCCAT GCTGGAGCGG ATGAAGCACG AGAAGACAGT GGACATCTAT 
GGCCACGTGA CATGCATGCG ATCGCAGCGG AACTACATGG TGCAGACAGA GGACCAGTAC 
GTGTTCATCC ACGAGGCGCT GCTAGAGGCC GCCACGTGCG GACACACGGA GGTGCCTGCC 
CGCAGCCTGT ATGCCCATAT CCAGAAGCTG GGCCAAGTGC CTCCCGGGGA GAGTGTGACT 
GCCATGGAGC TCGAGTTCAA GCTGCTGGCC AGCTCCAAGG CCCACACATC CCGCTTCATT 
AGCGCCAACC TGCCCTGCAA CAAGTTCAAG AACCGCCTGG TGAACATCAT GCCCTATGAG 
TTGACCCGTG TGTGTCTGCA GCCCATCCGT GGTGTGGAGG GCTCTGACTA CATCAATGCC 
AGCTTCTTGG ACGGCTATAG GCAGCAGAAG GCCTACATCG CCACGCAGGG GCCCCTGGCA 
GAGAGCACCG AGGACTTCTG GCGCATGCTC TGGGAGCACG GCTCCACCAT CGTCGTCATG 
CTGACCAGGC TTCGGGAGAT GGGCAGGGAG AAATGCCACC AGTACTGGCC AGCAGAGCGC 
TCCGCTCGCT ACCAATACTT TGTCGTTGAC CCGATGGCTG AGTACAACAT GCCGCAGTAC 
ATCCTGCGTG AGTTCAAGGT CACAGATGCC CGGGATGGGC AGTCGAGGAC AATCCGGCAG 
TTCCAGTTCA CAGACTGGCC CGAGCAGGGC GTGCCTAAGA CGGGTGAGGG CTTCATCGAC 
TTCATCGGGC AGGTGCACAA GACCAAGGAG CAGTTCGGGC AGGACGGGCC CATCACGGTG 
CACTGCAGTG CCGGCGTGGG CCGCACCGGG GTGTTCATCA CTCTGAGCAT CGTCCTGGAG 
CGGATGCGCT ACGAGGGCGT GGTCGACATG TTCCAGACCG TGAAGACCCT GCGCACACAG 
CGCCCAGCCA TGGTGCAGAC GGAGGACCAG TACCAGCTGT GCTATCGTGC GGCCCTGGAG 
TACCTTGGCA GCTTTGACCA CTATGCAACG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629174.1
CDS	125..5317
Translation	MAPAPAPRKTMVPLVPALVMLGLVAGAHGDSKPAFIKVPEDQTGLSGGVASFVCQATGEPKPRITWMKKGKKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEEQLPPGFPSIDMGPQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPATSNGRIKQLRSGGSPIRGALQIESSEESDQGKYECVATNSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGSVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPVGRNVLELSNVVRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLLVTETTATSVTLTWDSGNSEPVSYYGIQYRAAGTEGPFQEVDGVATTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRVQARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDARRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLAFTAVGDGPPSPTIQVKTQQGVPAQPADFQAEAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVTFDPTSSYTLEDLKPDTLYHFQLAARSEMGVGVFTPTVEARTAQSTPSAPPQKVTCVSTGSTTVRVSWVPPPAASRNGVITQYSVAYEAVDGEDRGRHVVESISREHSSWDLVGLEKWTEYRVWVRAHTDVGPGPESSPVLVRTDEDVPSGPPRKVEVEPLNSTAVRVSWKLPVPSKQHGQIRGYQVTYVRLENGEPRGPPIIQDVMLAEAQWRPEESEDYETIISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHSMRKLIADLQPNTEYSFVLMNRGSSAGGLQHLVSIRTAPDLLPHKPMPASAFIEDGRFTLSMPHVQDASLIRWFYIVVVPIDRVGGSVLAPRWSTPEELELDELLEAIEQGGGERRRRRQAERLKPYVAAQVDVLPESFTLGDKKSYRGFYNRPLSPDLSYQCFVLASLKEPVDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVLAVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGSSVPSCPNISSMRDHPPIPITDLADNIERLKANDGLKFSQEYESIDPGQQFTWENSNLEVNKPKNRYANVIAYDHSRVILTSVDGVPGSDYINANYIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRTATVVMMTRLEEKSRVKCDQYWPARGTETHGLIQVTLLDTVELATYTVRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCSAGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAATCGHTEVPARSLYAHIQKLGQVPPGESVTAMELEFKLLASSKAHTSRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGVEGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHGSTIVVMLTRLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQFGQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYLGSFDHYAT

Target ORF information:

RefSeq Version	XM_005629117.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein tyrosine phosphatase, receptor type F (PTPRF), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629117.3

ORF Insert Sequence:

ATGGCCCCTG CGCCAGCCCC ACGGAAGACG ATGGTGCCCC TTGTGCCTGC GCTCGTGATG 
CTTGGCTTGG TGGCAGGTGC CCATGGTGAC AGCAAACCTG CCTTCATTAA GGTCCCCGAG 
GACCAGACTG GGCTGTCGGG AGGAGTGGCC TCCTTCGTGT GCCAAGCCAC AGGGGAGCCC 
AAGCCACGCA TCACATGGAT GAAGAAGGGG AAGAAAGTCA GCTCCCAGCG CTTTGAGGTC 
ATTGAGTTTG ATGATGGGGC AGGGTCTGTG CTGCGGATCC AGCCATTGCG GGTGCAACGA 
GATGAAGCCA TCTACGAGTG CACAGCCACC AACAGTCTGG GAGAGATTAA CACGAGTGCC 
AAGCTCTCAG TTCTCGAAGA AGAACAGCTG CCCCCTGGGT TCCCCTCCAT CGACATGGGG 
CCTCAGCTGA AGGTGGTGGA GAAGGCGCGC ACAGCCACCA TGCTGTGCGC AGCAGGCGGG 
AATCCAGACC CTGAGATCTC TTGGTTCAAG GACTTCCTCC CTGTGGACCC TGCTACAAGC 
AATGGCCGCA TCAAGCAGCT ACGTTCAGGT GGTTCACCAA TCAGAGGTGC CTTGCAGATT 
GAGAGCAGTG AGGAGTCGGA CCAAGGCAAG TACGAGTGTG TGGCGACCAA CTCCGCAGGC 
ACACGCTACT CGGCCCCTGC CAACCTATAT GTACGAGTGC GCCGTGTGGC TCCTCGTTTC 
TCCATCCCTC CCAGCAGCCA GGAGGTGATG CCGGGTGGCA GCGTGAACCT GACTTGTGTT 
GCGGTGGGGG CACCCATGCC CTACGTGAAG TGGATGATGG GGGCCGAGGA GCTCACCAAG 
GAGGATGAGA TGCCAGTTGG CCGCAACGTG CTGGAGCTCA GCAATGTCGT GCGCTCTGCC 
AACTACACCT GCGTGGCCAT CTCCTCTCTA GGCATGATCG AGGCCACGGC CCAGGTCACA 
GTGAAAGCTC TGCCGAAACC TCCAATCGAC CTCCTGGTGA CTGAGACAAC TGCCACCAGC 
GTCACCCTGA CCTGGGACTC TGGGAACTCA GAGCCTGTGT CCTACTACGG CATCCAGTAC 
CGCGCGGCGG GGACGGAGGG CCCCTTCCAG GAGGTGGACG GTGTGGCCAC CACCCGCTAC 
AGCATTGGCG GCCTCAGCCC CTTCTCCGAG TACGCCTTCC GCGTGCTGGC GGTGAACAGC 
ATCGGCCGGG GACCACCCAG CGAGGCGGTG CGGGCACGCA CGGGAGAGCA GGCGCCCTCC 
AGCCCCCCGC GCCGCGTGCA GGCACGCATG CTGAGCGCGA GCACCATGCT GGTGCAGTGG 
GAGCCGCCCG AGGAGCCCAA CGGCCTGGTG CGAGGGTACC GCGTCTACTA CACTCCCGAC 
GCCCGCCGCC CCCTGAGCGC CTGGCACAAG CACAACACGG ACGCGGGCCT CCTCACCACC 
GTGGGCAGCC TGCTGCCCGG AATCACCTAC AGCCTGCGCG TGCTGGCCTT CACCGCCGTG 
GGCGACGGCC CTCCCAGCCC CACCATCCAG GTCAAGACAC AGCAGGGAGT GCCTGCGCAA 
CCGGCGGACT TCCAGGCTGA AGCAGAGTCG GACACCAGGA TCCAGCTCTC GTGGCTGCTT 
CCCCCGCAGG AGCGGATCAT CAAGTACGAG CTGGTGTATT GGGCAGCAGA GGATGAAGGC 
CAGCAGCACA AGGTGACCTT CGACCCCACC TCCTCCTACA CCCTGGAGGA CCTGAAGCCT 
GACACACTGT ACCACTTCCA GCTGGCCGCC CGTTCCGAGA TGGGGGTGGG CGTGTTCACC 
CCCACCGTTG AGGCCCGCAC AGCACAGTCC ACCCCCTCAG CCCCTCCCCA GAAGGTGACC 
TGTGTGAGCA CGGGCTCCAC CACGGTTCGG GTAAGTTGGG TCCCGCCGCC AGCTGCCAGC 
CGCAACGGCG TCATCACCCA GTACTCGGTG GCCTACGAGG CAGTGGACGG CGAGGACCGA 
GGGCGGCATG TGGTGGAGAG CATCAGTCGC GAGCACTCCA GCTGGGACCT GGTGGGCCTG 
GAGAAGTGGA CGGAGTACCG GGTGTGGGTG CGGGCGCACA CTGACGTGGG CCCCGGCCCA 
GAGAGCAGCC CGGTGCTCGT GCGCACAGAC GAGGACGTGC CCAGTGGACC GCCACGGAAG 
GTGGAGGTGG AACCACTGAA TTCCACTGCT GTGCGTGTCT CTTGGAAGCT GCCCGTCCCC 
AGCAAGCAGC ACGGCCAGAT TCGCGGCTAC CAGGTCACCT ATGTGCGGCT AGAGAATGGC 
GAGCCCCGTG GCCCACCCAT CATCCAGGAC GTCATGCTGG CTGAGGCCCA GTGGCGGCCA 
GAGGAGTCCG AGGACTATGA AACCATAATC AGCGGCCTGA CCCCAGAGAC CACCTATTCC 
ATTACCGTCG CCGCCTACAC CACCAAAGGG GATGGTGCCC GCAGCAAGCC CAAAGTTGTC 
ACAACGACCG GGGCAGTGTT TGCCAAGAAC TTCCGTGTGG CGGCCGCTAT GAAGACGTCT 
GTGCTGCTCA GCTGGGAGGT CCCAGACTCC TACAAGTCGG CTGTACCCTT CAAGATCCTG 
TACAACGGGC AGAGTGTGGA GGTGGACGGG CACTCCATGC GGAAACTGAT CGCAGACCTG 
CAGCCGAACA CGGAGTACTC GTTCGTGCTG ATGAACCGTG GCAGCAGCGC GGGGGGCCTG 
CAGCACCTCG TGTCCATCCG CACGGCCCCA GACCTCCTGC CACACAAGCC GATGCCCGCC 
TCCGCCTTCA TAGAGGATGG TCGCTTCACC CTCTCCATGC CCCACGTGCA GGATGCCTCA 
CTCATCAGGT GGTTCTACAT CGTGGTGGTA CCCATCGACC GCGTGGGTGG GAGTGTGCTG 
GCCCCGAGAT GGAGCACGCC TGAGGAGCTG GAGCTGGACG AGCTTCTGGA GGCCATCGAG 
CAGGGCGGTG GGGAGCGGCG ACGACGGCGG CAGGCGGAGC GGCTGAAGCC ATATGTGGCT 
GCACAGGTGG ACGTGCTCCC CGAGAGCTTC ACCCTAGGAG ACAAGAAGAG CTACCGGGGC 
TTCTACAACC GGCCTCTGTC TCCGGACCTG AGCTATCAGT GCTTTGTGCT CGCCTCCCTG 
AAGGAACCCG TGGACCAGAA GCGCTATGCC TCCAGCCCCT ACTCTGATGA GATCGTGGTC 
CAAGTGACAC CAGCCCAGCA GCAGGAGGAG CCTGAGATGC TGTGGGTGAC AGGCCCCGTC 
CTCGCAGTCA TCCTCATCAT CCTCATTGTC ATCGCCATCC TCCTGTTCAA GAGGAAGAGG 
ACTCACTCCC CATCATCCAA GGACGAGCAG TCAATCGGGC TGAAGGACTC CTTGCTGGCC 
CATTCCTCTG ACCCTGTGGA GATGCGGAGG CTCAACTACC AGACTCCAGG TTCCAGTGTC 
CCCAGTTGCC CGAATATCTC AAGTATGCGA GACCACCCGC CCATCCCCAT CACTGACCTG 
GCCGACAACA TTGAGCGCCT CAAAGCCAAC GACGGCCTCA AGTTCTCCCA GGAGTACGAG 
TCCATTGACC CTGGACAGCA GTTCACCTGG GAGAATTCAA ACCTGGAAGT GAACAAGCCC 
AAGAACCGCT ATGCAAACGT CATCGCCTAC GACCACTCCC GAGTCATCCT TACCTCCGTC 
GATGGTGTCC CAGGGAGTGA CTATATCAAC GCCAACTACA TCGATGGCTA CCGCAAGCAG 
AACGCCTACA TTGCCACGCA GGGCCCACTG CCCGAGACCA TGGGCGATTT CTGGCGGATG 
GTGTGGGAAC AGCGCACAGC CACCGTGGTC ATGATGACAC GGCTGGAGGA GAAGTCTCGG 
GTGAAGTGTG ATCAGTACTG GCCAGCCCGG GGTACCGAGA CCCACGGCCT CATTCAGGTG 
ACCCTGCTGG ACACGGTGGA GCTGGCCACA TACACTGTGC GCACCTTTGC ACTCCACAAG 
AGTGGCTCCA GCGAGAAGCG GGAGCTGCGT CAGTTCCAGT TCATGGCCTG GCCGGACCAC 
GGGGTTCCCG AGTACCCCAC CCCCATCCTG GCCTTCCTGC GGCGGGTCAA GGCCTGTAAC 
CCGCTAGACG CAGGGCCCAT GGTGGTGCAC TGCAGCGCGG GCGTGGGCCG CACGGGCTGT 
TTCATCGTCA TCGACGCCAT GCTGGAGCGG ATGAAGCACG AGAAGACAGT GGACATCTAT 
GGCCACGTGA CATGCATGCG ATCGCAGCGG AACTACATGG TGCAGACAGA GGACCAGTAC 
GTGTTCATCC ACGAGGCGCT GCTAGAGGCC GCCACGTGCG GACACACGGA GGTGCCTGCC 
CGCAGCCTGT ATGCCCATAT CCAGAAGCTG GGCCAAGTGC CTCCCGGGGA GAGTGTGACT 
GCCATGGAGC TCGAGTTCAA GCTGCTGGCC AGCTCCAAGG CCCACACATC CCGCTTCATT 
AGCGCCAACC TGCCCTGCAA CAAGTTCAAG AACCGCCTGG TGAACATCAT GCCCTATGAG 
TTGACCCGTG TGTGTCTGCA GCCCATCCGT GGTGTGGAGG GCTCTGACTA CATCAATGCC 
AGCTTCTTGG ACGGCTATAG GCAGCAGAAG GCCTACATCG CCACGCAGGG GCCCCTGGCA 
GAGAGCACCG AGGACTTCTG GCGCATGCTC TGGGAGCACG GCTCCACCAT CGTCGTCATG 
CTGACCAGGC TTCGGGAGAT GGGCAGGGAG AAATGCCACC AGTACTGGCC AGCAGAGCGC 
TCCGCTCGCT ACCAATACTT TGTCGTTGAC CCGATGGCTG AGTACAACAT GCCGCAGTAC 
ATCCTGCGTG AGTTCAAGGT CACAGATGCC CGGGATGGGC AGTCGAGGAC AATCCGGCAG 
TTCCAGTTCA CAGACTGGCC CGAGCAGGGC GTGCCTAAGA CGGGTGAGGG CTTCATCGAC 
TTCATCGGGC AGGTGCACAA GACCAAGGAG CAGTTCGGGC AGGACGGGCC CATCACGGTG 
CACTGCAGTG CCGGCGTGGG CCGCACCGGG GTGTTCATCA CTCTGAGCAT CGTCCTGGAG 
CGGATGCGCT ACGAGGGCGT GGTCGACATG TTCCAGACCG TGAAGACCCT GCGCACACAG 
CGCCCAGCCA TGGTGCAGAC GGAGGACCAG TACCAGCTGT GCTATCGTGC GGCCCTGGAG 
TACCTTGGCA GCTTTGACCA CTATGCAACG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PTPRF
Entrez Gene ID	475389
Full Name	protein tyrosine phosphatase, receptor type F
General protein information	Preferred Names protein tyrosine phosphatase, receptor type F Names receptor-type tyrosine-protein phosphatase F
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Rattus norvegicus (Norway rat)	Ptprf	NP_062122.1
Danio rerio (zebrafish)	ptprfb	NP_001077045.1
Danio rerio (zebrafish)	ptprfa	XP_692206.6
	PTPRF	XP_001095521.2
Pan troglodytes (chimpanzee)	PTPRF	XP_001173898.2
Canis lupus familiaris (dog)	PTPRF	XP_005629167.1
Gallus gallus (chicken)	PTPRF	XP_004936750.1
Homo sapiens (human)	PTPRF	NP_002831.2
Mus musculus (house mouse)	Ptprf	NP_035343.2
Bos taurus (cattle)	PTPRF	NP_001094549.1
Xenopus tropicalis (tropical clawed frog)	ptprf	XP_004914019.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PTPRF cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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