plce1 cDNA ORF clone, Danio rerio(zebrafish)

The following plce1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plce1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa148226 XM_021480153.1
Latest version!
Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa45396 XM_009306427.2
Latest version!
Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa35532 NM_001161653.1
Latest version!
Danio rerio phospholipase C, epsilon 1 (plce1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa148220 XM_021480147.1
Latest version!
Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa148221 XM_021480148.1
Latest version!
Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa148222 XM_021480149.1
Latest version!
Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa148223 XM_021480150.1
Latest version!
Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa148224 XM_021480151.1
Latest version!
Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa148225 XM_021480152.1
Latest version!
Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa148227 XM_021480154.1
Latest version!
Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa45393 XM_009306422.2
Latest version!
Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa45394 XM_009306425.2
Latest version!
Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa45395 XM_009306426.2
Latest version!
Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa45397 XM_009306428.2
Latest version!
Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa148226
    Clone ID Related Accession (Same CDS sequence) XM_021480153.1
    Accession Version XM_021480153.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGTGGCTGA GCTCCTCTCG CCGCTGCGGT GTGAAGCAGG GAGCCAGCGA GACCACCACA 
    ACCATGAAAC ACTCCCACTC GCAGAGGATG GACTACAGGC TGCTCAAACG GCAGCTGTGC
    AGCTCCATGG CCAACTCCAG CATCCTGCCA TCTGCTGTGA CGGGGATCAG TAAGGAACTA
    GCTGACCTGC GCCACCTCAT CCAGTTTCCA GAGGAAATCG CCACCATCCT GACAGAACAG
    GAGCAGCAGC TATACCGCCG CGTCTTCCCC CTCGACTACC TCAGCTTTCT CACCCGAGAC
    CTGGGCAGCC CAGAGTGCCA CAAACGCCAC CCGCATCTAA AAGCTTCCCT GTCAGCCCCC
    ATCATGCCCA CTCAGAATGA CAACCACAAC ACTGTTGAAG ACCTCGTCAC AAGATTCAAT
    GAGGTGAGCT CATGGGTGAC CTGGCTTATC CTGACGGCGG GCTCCATGGA GGAGAAGAGG
    GAGGTTTTCT CGTACCTCGT CCATGTGGCC AAGTGCTCGT GGAACATGGG CAACTACAAC
    GCGGTCATGG AGTTCTTGGC AGGCCTCAGG TCCCGCAAAG TGCTGAAGAT GTGGCAGTTT
    ATGGATCAGG CAGATATTGA GACCATGCGA GGTCTAAAGG ATGCCATGGC CCAGCACGAA
    TCATCATCTG AGTACAAGAA AGTCGTCAGC CGTGCCCTCA ATATCCCAGG ATGCAAAGTG
    GTGCCTTTCT GTGGCGTGTT CCTCAAGGAG CTGAGTGAGG CTCTGGATGG CGCGGCCAGC
    ATTATTGGAC TGCGTCCGTC TTTTGATTCT CAAGAAGATC CTGTTGAGTT TGTCACGGAC
    TACAACGGCC AGCAGCACTT TCTGCAGAGG CTTGGAAGTG ATGGACTTCA CAGCTCAGAT
    AAAGAGGCGA CCGTCAGTAA CATACTGCAG ACCATCCGCA GCTGCAACCG AAGCCTGGAG
    GCTGAGGAGC CTGAGGAGAG GGCACGAGAG ATCACCGTTT GCCCCAAAAA TTCCTTTAAA
    GACAAGAGCA GAAACCAGAA AGGACCTGGG ACTGAACGAG TCCTGTTTTC AATCGGCGAC
    CTCTCAGACT CTGAAGGGGA CCCACTTGAG ACAGTTAAGG ATGTTGTAGA CCTCCAGACC
    ACCGAAGATG TTCGCGGACC CTTTAGCCAT GGGACTGAGC TCATTCCCTG GTACGTTCTG
    TCGCTTCAGC CGGATATCCA TCAGTTCCTG CTCCAAGGAG CAACCGTCAT CCACTACGAC
    CCCGAGAGCC ACCTCACCGC CCGCTGTCTT CTCCGTCTAC AGCCCGATAA CTGCTTCCTC
    ACCTGGTGCA AACCCCACAG TAGCTGCAGC CTATATGGAA GAGCTAGAAC CTTCATGGGA
    CATCCAGCAT CCCCAGACCA CTTACACATC GGCCAACCTG TTCACTGCGG ACTCTCTGAC
    GGGCTCCTGG ATCTTAATGT GGTGAAGGCT GTGTTTATGG GTCACCCTGG AGTGGACGTC
    AACTATGTCT GCCTGCAGCA CAAACTGTGT AACATGAATC CTGGTGAAAA TGGTGTCACG
    CTGCTATATG GACTTCACAC CACAGACAAC AGGCTCCTGC ACTTTGTGGC CCCGAAACAC
    ACGGCTCGCA TGCTGCACGA GGGCCTCCAG GAGCTGCTGA ACTCTATTAG GAAGATCAGG
    AAATTCCCTG ACCAGAGACT GCAATGGCTG AGAAAGCAAT ATGTTAGTTT GTACCAGGAG
    GATGGTAGAT TTGAAGGACC TACACTGGCT CATGCTATCG AGCTGTTCGG AGGGAGACGC
    TGGAATATGG GAACTAGCGG CCCTGGATCC GCCAGCAGAG GTGCAGAGAA GAACTCGGCT
    CAGAAGAACA GTCCACTGGG CATCAACTCC AATGTCAAAA AAAAGAAAAA GGCCTTAGTC
    AGGGGGGACA GTGGCGATGG AACTGATGAT GAGATGACCG CACGCAAGAC CAGGAGTTGC
    AAAGAGACTT TGGGGAGGAG AGAGTCAGAT GCTCTGGAAA ATGTTGAGCA GGAAGAGGCT
    GAGGACTGCA GTACCTTTAG CCAACCTGGA CCATTTTCCT TATCCAACAA CAAAGGCCAG
    TCTCCGGGAT CCTCCTCGTC CTCCTCCACC TCTTCTTCTG TTGCCTCCTT TTGCACAAAC
    TCCTCCACCC CTACACGTCC ACATTCCTCC CCAATCCTCC CCACAAACCT CAAGAGTCAG
    CCAGGGGCCT GGAGCAGTCG AAGCTGGCAC GGTCGAGGGA AAGGCTGCTT CAGAGGTTTC
    CAGGACCTCA TGATCTCCGA CAGCATCATG AGCTTTGTCG AATTTGTGGA GCTCTTCAAA
    TCTTTTAGCA TTCGCAGTCG TAAGGATCTG AAGGAACTAT TCGACACCTA CGCCGTGCCC
    TGCAGTCGCT CTGGCCCTGA GTCTGTCCCC CTCTACACCA CACTTAGGAT TGACGACAAG
    CTAACCGGAC TGCAGCCAGA TCTGGATCTC CTCACTCGCA ACGGCTCTGA CCTGGGCCTT
    TTCATTCGCA CCCGCCAGCA AATGTCTGAA AACCAGAAGC AGATTTCAGA CGCCATCGCA
    GCTGCCAGTA TTGTGACCAA TGGCACTGGA GTGGAGAATT CATCGCTGGG TGTGCTGGGA
    CTGGCCATCT CGCAGCTCAA TGATTTCTTG GTCAACTGTC AGAGAGAACA TCTGAGCTAT
    GATGAGATCC TCAGCATCAT ACAGAAATTT GAGCCTTCTT CAAACATGCG TCAGATGGGG
    TGGATGTCGT TTGAAGGATT CGCCAGGTTT CTAATGGACA AGGACAACTT TGCCTCCAAA
    AATGAGGAGT CTCAAGTGAA CCTGGATGAG CTCCAGCATC CGCTGTCTTA CTATTACATC
    GAGTCCTCTC ATAACACATA CCTGACGGGG CATCAGCTGA AGGGAGAGTC TTCAGTCGAG
    CTCTACAGCC AGGTGTTGCT GCAGGGTTGT CGCAGCGTTG AGTTGGACTG CTGGGATGGG
    GATGACGGCA TGCCTGTTAT TTACCATGGA CATACTCTTA CCACTAAGAT TCCTTTCAAG
    GATGTTGTAG AGGCCATCAA CCGTGCAGCA TTTGTGAACT CAGAGATGCC TGTGATTCTG
    TCCATTGAAA ACCACTGCTC CTTACCACAG CAGCGCAAAA TGGCTGAGAT TTTTAAGATG
    GTGTTTGGTG AGAAGCTGGT GACCAAGTTT CTGTTTGAAA GTGATTTCGC GGACGAGCCT
    TTGCTTCCGT CTCCCCTTCA GCTCCGGGGG AAAATCCTCC TGAAGAATAA AAAGTTAAAA
    GCTCACCAGG CTCCTGTTGA CATTCTCAAA CAAAAGGCGC ATCAGCTTGC CCACATGCAG
    GCTCAGGCCA ACAACGGCAC AGTCAGCACA ACGCCACTGG GAAACAATGA TGAAGAGGAG
    GAGGAGGAAG ACGAGTACGA TTACGATTAC GAGTCGCTGT CTGACGCTGA TGTCCTCAAT
    GCTTCCACAG CCTCTTATGG CCAGGAAGAC AATATTCTGG ATGACAAACC TGAAGGCAAG
    TCATCTACGG AGAAACTTCA GTACGAGTCC AACGATGAGA TGCCAAAGCG GTTTAAAAAA
    GCTGGAAGCA AAGGAAAGAT GTTTGACATG GAGCTGGGAG AGGAGTTTTA CCTGCCCCAG
    AACGAGAAGG AGAGTAGACA GATTGCTCAA GAGCTGTCAG ATCTGGTCAT CTACTGCCAG
    GCTATCAAAT TTCCAGGCCT CTCCACCCTC TCTCCATCAG GCTCTGGAAG GGGGAAAGAC
    AGAAAGAGCC GGAAATCCAT CTTTGGCAGC GCTCCTGCTC GCGGGAGCCC CGGGGAGCCT
    GTGACGCTGG TCCGAGCTCC AGGGAAAGCC AGTCTTGAGG GCATTCGCAT GAACTCGGAG
    GATCAATTGT GTCTGAGCCC GAGCACCTCT CTGAGCTCCA TCATCCGCAC GCCCAAATGC
    TACCACATCT CCTCTGTGAA CGAGAATGCA GCGAAGCGCT TGTGTCGCCG CTACTCGCAG
    AAGCTGATCC AGCACACCAG CTGCCAGCTG CTGCGCACGT ACCCAGCGGC CACACGCATT
    GACTCTGCCA ACCCCAATCC GCTCATCTTC TGGCTGCACG GAGTCCAGCT GGTGGCCCTC
    AACTACCAGA CTGATGATCT GCCGATGCAG CTGAACGCGG CTCTATTCGA GGCTAATGGT
    CACTGTGGTT TTGTGCTGAA GCCTCCAGTC CTGTGGGACA GAAGCTGTCC TCTCTATCAG
    CACTTTTACC CTCTGGACCG AGACCTGGAG AACATGACCC CAACGCTATA CACACTCACT
    ATTGTGTCGG GGCAGAACGT GTGTCCAGGA AACTCAAACG GCAGTCCGTG TGTCGAAGTG
    GAGGTGTTGG GAATGCCGGC GGACAGCTGT CATTTCCGAA CTAAACCCAT ACACCGCAAC
    ACACTCAATC CCATGTGGAA CGAACACTTC CAGTTCCACG TGCACTTTGA AGACCTGGCG
    TTCCTGCGCA TCGCTGTGGT GGAGAACAAC AGCTCTCAGG TCACCGCTCA GAGGATACTT
    CCCCTCAAAA CACTCAGAGC AGGCTACCGG CACCTGCAGC TGAGGAACCT GCACAACGAG
    CCTCTGGAGG TGTCCAGTCT GTTCATGTTC AGCCGCAGGA CGGAGGAGAG CCCGACCGGA
    GGACCACCGT CTGCGTCTGT GCTGTTCAGC ACCGAGGAGA GACGATCTGT GCAGCAGCAC
    AAGGTGACCG TGCATGGAGT CCCCGGACCT GAACCCTTCA CTGTGATCTG TGTCGATGAA
    TTCACCACAG CCAAACAGCT CCTGGACTCT CTGTTTCCGA CTGCCTCTTT TAAGTATATG
    CTGATGGAGG AGAGAGTGTC TCTGTCGAAG GAGAAGAAGG CTCCTCAGCA GCGTCCTCTA
    GCCAATGATG AGAGACTGCT GAAGCTCATT CACAGCTGGC AGCCTGAGGA CGGATATGTG
    GGCAGAATAT ACCTCAAAAC CAGAGAACAG CAGAACTGCA GTGAGAAGAC GAGCGTCCCG
    CTGGAGTCAC TGGAGGAGCT CGGCAGCTTA GAGGACGACA CCTTCTTCGT GCAGGTCCAT
    GATGTTTCTC CAGAACAGCC TCACACCGTC ATCAAAGCCC CGCGATACAG CACAGCACAG
    GACATCATCC AGCAGACTCT GAGCAAAGCC AAGTATTCTC TGAGTATCCT GAGCAACCCC
    AACCCATGTG ACTACGTCCT GATGGAGGAG CTTTGTAAAG ACGCGGGTGG AAAGAAGTCG
    TCCTCTGCCA AACCGTGCCA GCGTGTGCTG CAGGACCACG AGTGTGTGTT TCAGGCTCAG
    AGCCGCTGGA AGGGGGCTGG AAAATTCATC CTCAAACTCA AAGAGCAGTT GGCTCGTGAG
    GACAAGCGTA AAGGTGTGTC GTTCGCCAGT GAGCTGAGGA AGCTGACGGG TCGCAGTCGC
    AGTGTGACGG TCAACACAAA CAGCAACGAC ACTCACAGGT CTCTGTCTCT GGGGCTGAAG
    GCCTGGATGG CTGAGGGCCG CTTGGGCTCT CCGAGACGCT CCCAGTGTGG TGACGGTGTC
    TCGGCCTTGT CCGGGTGGTC TCTCCCGCTG CTGTGGAACT GGAAGCTGCA TAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335828.1
    CDS71..5707
    Translation

    Target ORF information:

    RefSeq Version XM_021480153.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480153.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGTGGCTGA GCTCCTCTCG CCGCTGCGGT GTGAAGCAGG GAGCCAGCGA GACCACCACA 
    ACCATGAAAC ACTCCCACTC GCAGAGGATG GACTACAGGC TGCTCAAACG GCAGCTGTGC
    AGCTCCATGG CCAACTCCAG CATCCTGCCA TCTGCTGTGA CGGGGATCAG TAAGGAACTA
    GCTGACCTGC GCCACCTCAT CCAGTTTCCA GAGGAAATCG CCACCATCCT GACAGAACAG
    GAGCAGCAGC TATACCGCCG CGTCTTCCCC CTCGACTACC TCAGCTTTCT CACCCGAGAC
    CTGGGCAGCC CAGAGTGCCA CAAACGCCAC CCGCATCTAA AAGCTTCCCT GTCAGCCCCC
    ATCATGCCCA CTCAGAATGA CAACCACAAC ACTGTTGAAG ACCTCGTCAC AAGATTCAAT
    GAGGTGAGCT CATGGGTGAC CTGGCTTATC CTGACGGCGG GCTCCATGGA GGAGAAGAGG
    GAGGTTTTCT CGTACCTCGT CCATGTGGCC AAGTGCTCGT GGAACATGGG CAACTACAAC
    GCGGTCATGG AGTTCTTGGC AGGCCTCAGG TCCCGCAAAG TGCTGAAGAT GTGGCAGTTT
    ATGGATCAGG CAGATATTGA GACCATGCGA GGTCTAAAGG ATGCCATGGC CCAGCACGAA
    TCATCATCTG AGTACAAGAA AGTCGTCAGC CGTGCCCTCA ATATCCCAGG ATGCAAAGTG
    GTGCCTTTCT GTGGCGTGTT CCTCAAGGAG CTGAGTGAGG CTCTGGATGG CGCGGCCAGC
    ATTATTGGAC TGCGTCCGTC TTTTGATTCT CAAGAAGATC CTGTTGAGTT TGTCACGGAC
    TACAACGGCC AGCAGCACTT TCTGCAGAGG CTTGGAAGTG ATGGACTTCA CAGCTCAGAT
    AAAGAGGCGA CCGTCAGTAA CATACTGCAG ACCATCCGCA GCTGCAACCG AAGCCTGGAG
    GCTGAGGAGC CTGAGGAGAG GGCACGAGAG ATCACCGTTT GCCCCAAAAA TTCCTTTAAA
    GACAAGAGCA GAAACCAGAA AGGACCTGGG ACTGAACGAG TCCTGTTTTC AATCGGCGAC
    CTCTCAGACT CTGAAGGGGA CCCACTTGAG ACAGTTAAGG ATGTTGTAGA CCTCCAGACC
    ACCGAAGATG TTCGCGGACC CTTTAGCCAT GGGACTGAGC TCATTCCCTG GTACGTTCTG
    TCGCTTCAGC CGGATATCCA TCAGTTCCTG CTCCAAGGAG CAACCGTCAT CCACTACGAC
    CCCGAGAGCC ACCTCACCGC CCGCTGTCTT CTCCGTCTAC AGCCCGATAA CTGCTTCCTC
    ACCTGGTGCA AACCCCACAG TAGCTGCAGC CTATATGGAA GAGCTAGAAC CTTCATGGGA
    CATCCAGCAT CCCCAGACCA CTTACACATC GGCCAACCTG TTCACTGCGG ACTCTCTGAC
    GGGCTCCTGG ATCTTAATGT GGTGAAGGCT GTGTTTATGG GTCACCCTGG AGTGGACGTC
    AACTATGTCT GCCTGCAGCA CAAACTGTGT AACATGAATC CTGGTGAAAA TGGTGTCACG
    CTGCTATATG GACTTCACAC CACAGACAAC AGGCTCCTGC ACTTTGTGGC CCCGAAACAC
    ACGGCTCGCA TGCTGCACGA GGGCCTCCAG GAGCTGCTGA ACTCTATTAG GAAGATCAGG
    AAATTCCCTG ACCAGAGACT GCAATGGCTG AGAAAGCAAT ATGTTAGTTT GTACCAGGAG
    GATGGTAGAT TTGAAGGACC TACACTGGCT CATGCTATCG AGCTGTTCGG AGGGAGACGC
    TGGAATATGG GAACTAGCGG CCCTGGATCC GCCAGCAGAG GTGCAGAGAA GAACTCGGCT
    CAGAAGAACA GTCCACTGGG CATCAACTCC AATGTCAAAA AAAAGAAAAA GGCCTTAGTC
    AGGGGGGACA GTGGCGATGG AACTGATGAT GAGATGACCG CACGCAAGAC CAGGAGTTGC
    AAAGAGACTT TGGGGAGGAG AGAGTCAGAT GCTCTGGAAA ATGTTGAGCA GGAAGAGGCT
    GAGGACTGCA GTACCTTTAG CCAACCTGGA CCATTTTCCT TATCCAACAA CAAAGGCCAG
    TCTCCGGGAT CCTCCTCGTC CTCCTCCACC TCTTCTTCTG TTGCCTCCTT TTGCACAAAC
    TCCTCCACCC CTACACGTCC ACATTCCTCC CCAATCCTCC CCACAAACCT CAAGAGTCAG
    CCAGGGGCCT GGAGCAGTCG AAGCTGGCAC GGTCGAGGGA AAGGCTGCTT CAGAGGTTTC
    CAGGACCTCA TGATCTCCGA CAGCATCATG AGCTTTGTCG AATTTGTGGA GCTCTTCAAA
    TCTTTTAGCA TTCGCAGTCG TAAGGATCTG AAGGAACTAT TCGACACCTA CGCCGTGCCC
    TGCAGTCGCT CTGGCCCTGA GTCTGTCCCC CTCTACACCA CACTTAGGAT TGACGACAAG
    CTAACCGGAC TGCAGCCAGA TCTGGATCTC CTCACTCGCA ACGGCTCTGA CCTGGGCCTT
    TTCATTCGCA CCCGCCAGCA AATGTCTGAA AACCAGAAGC AGATTTCAGA CGCCATCGCA
    GCTGCCAGTA TTGTGACCAA TGGCACTGGA GTGGAGAATT CATCGCTGGG TGTGCTGGGA
    CTGGCCATCT CGCAGCTCAA TGATTTCTTG GTCAACTGTC AGAGAGAACA TCTGAGCTAT
    GATGAGATCC TCAGCATCAT ACAGAAATTT GAGCCTTCTT CAAACATGCG TCAGATGGGG
    TGGATGTCGT TTGAAGGATT CGCCAGGTTT CTAATGGACA AGGACAACTT TGCCTCCAAA
    AATGAGGAGT CTCAAGTGAA CCTGGATGAG CTCCAGCATC CGCTGTCTTA CTATTACATC
    GAGTCCTCTC ATAACACATA CCTGACGGGG CATCAGCTGA AGGGAGAGTC TTCAGTCGAG
    CTCTACAGCC AGGTGTTGCT GCAGGGTTGT CGCAGCGTTG AGTTGGACTG CTGGGATGGG
    GATGACGGCA TGCCTGTTAT TTACCATGGA CATACTCTTA CCACTAAGAT TCCTTTCAAG
    GATGTTGTAG AGGCCATCAA CCGTGCAGCA TTTGTGAACT CAGAGATGCC TGTGATTCTG
    TCCATTGAAA ACCACTGCTC CTTACCACAG CAGCGCAAAA TGGCTGAGAT TTTTAAGATG
    GTGTTTGGTG AGAAGCTGGT GACCAAGTTT CTGTTTGAAA GTGATTTCGC GGACGAGCCT
    TTGCTTCCGT CTCCCCTTCA GCTCCGGGGG AAAATCCTCC TGAAGAATAA AAAGTTAAAA
    GCTCACCAGG CTCCTGTTGA CATTCTCAAA CAAAAGGCGC ATCAGCTTGC CCACATGCAG
    GCTCAGGCCA ACAACGGCAC AGTCAGCACA ACGCCACTGG GAAACAATGA TGAAGAGGAG
    GAGGAGGAAG ACGAGTACGA TTACGATTAC GAGTCGCTGT CTGACGCTGA TGTCCTCAAT
    GCTTCCACAG CCTCTTATGG CCAGGAAGAC AATATTCTGG ATGACAAACC TGAAGGCAAG
    TCATCTACGG AGAAACTTCA GTACGAGTCC AACGATGAGA TGCCAAAGCG GTTTAAAAAA
    GCTGGAAGCA AAGGAAAGAT GTTTGACATG GAGCTGGGAG AGGAGTTTTA CCTGCCCCAG
    AACGAGAAGG AGAGTAGACA GATTGCTCAA GAGCTGTCAG ATCTGGTCAT CTACTGCCAG
    GCTATCAAAT TTCCAGGCCT CTCCACCCTC TCTCCATCAG GCTCTGGAAG GGGGAAAGAC
    AGAAAGAGCC GGAAATCCAT CTTTGGCAGC GCTCCTGCTC GCGGGAGCCC CGGGGAGCCT
    GTGACGCTGG TCCGAGCTCC AGGGAAAGCC AGTCTTGAGG GCATTCGCAT GAACTCGGAG
    GATCAATTGT GTCTGAGCCC GAGCACCTCT CTGAGCTCCA TCATCCGCAC GCCCAAATGC
    TACCACATCT CCTCTGTGAA CGAGAATGCA GCGAAGCGCT TGTGTCGCCG CTACTCGCAG
    AAGCTGATCC AGCACACCAG CTGCCAGCTG CTGCGCACGT ACCCAGCGGC CACACGCATT
    GACTCTGCCA ACCCCAATCC GCTCATCTTC TGGCTGCACG GAGTCCAGCT GGTGGCCCTC
    AACTACCAGA CTGATGATCT GCCGATGCAG CTGAACGCGG CTCTATTCGA GGCTAATGGT
    CACTGTGGTT TTGTGCTGAA GCCTCCAGTC CTGTGGGACA GAAGCTGTCC TCTCTATCAG
    CACTTTTACC CTCTGGACCG AGACCTGGAG AACATGACCC CAACGCTATA CACACTCACT
    ATTGTGTCGG GGCAGAACGT GTGTCCAGGA AACTCAAACG GCAGTCCGTG TGTCGAAGTG
    GAGGTGTTGG GAATGCCGGC GGACAGCTGT CATTTCCGAA CTAAACCCAT ACACCGCAAC
    ACACTCAATC CCATGTGGAA CGAACACTTC CAGTTCCACG TGCACTTTGA AGACCTGGCG
    TTCCTGCGCA TCGCTGTGGT GGAGAACAAC AGCTCTCAGG TCACCGCTCA GAGGATACTT
    CCCCTCAAAA CACTCAGAGC AGGCTACCGG CACCTGCAGC TGAGGAACCT GCACAACGAG
    CCTCTGGAGG TGTCCAGTCT GTTCATGTTC AGCCGCAGGA CGGAGGAGAG CCCGACCGGA
    GGACCACCGT CTGCGTCTGT GCTGTTCAGC ACCGAGGAGA GACGATCTGT GCAGCAGCAC
    AAGGTGACCG TGCATGGAGT CCCCGGACCT GAACCCTTCA CTGTGATCTG TGTCGATGAA
    TTCACCACAG CCAAACAGCT CCTGGACTCT CTGTTTCCGA CTGCCTCTTT TAAGTATATG
    CTGATGGAGG AGAGAGTGTC TCTGTCGAAG GAGAAGAAGG CTCCTCAGCA GCGTCCTCTA
    GCCAATGATG AGAGACTGCT GAAGCTCATT CACAGCTGGC AGCCTGAGGA CGGATATGTG
    GGCAGAATAT ACCTCAAAAC CAGAGAACAG CAGAACTGCA GTGAGAAGAC GAGCGTCCCG
    CTGGAGTCAC TGGAGGAGCT CGGCAGCTTA GAGGACGACA CCTTCTTCGT GCAGGTCCAT
    GATGTTTCTC CAGAACAGCC TCACACCGTC ATCAAAGCCC CGCGATACAG CACAGCACAG
    GACATCATCC AGCAGACTCT GAGCAAAGCC AAGTATTCTC TGAGTATCCT GAGCAACCCC
    AACCCATGTG ACTACGTCCT GATGGAGGAG CTTTGTAAAG ACGCGGGTGG AAAGAAGTCG
    TCCTCTGCCA AACCGTGCCA GCGTGTGCTG CAGGACCACG AGTGTGTGTT TCAGGCTCAG
    AGCCGCTGGA AGGGGGCTGG AAAATTCATC CTCAAACTCA AAGAGCAGTT GGCTCGTGAG
    GACAAGCGTA AAGGTGTGTC GTTCGCCAGT GAGCTGAGGA AGCTGACGGG TCGCAGTCGC
    AGTGTGACGG TCAACACAAA CAGCAACGAC ACTCACAGGT CTCTGTCTCT GGGGCTGAAG
    GCCTGGATGG CTGAGGGCCG CTTGGGCTCT CCGAGACGCT CCCAGTGTGG TGACGGTGTC
    TCGGCCTTGT CCGGGTGGTC TCTCCCGCTG CTGTGGAACT GGAAGCTGCA TAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45396
    Clone ID Related Accession (Same CDS sequence) XM_009306427.2
    Accession Version XM_009306427.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5529bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTGGCTGA GCTCCTCTCG CCGCTGCGGT GTGAAGCAGG GAGCCAGCGA GACCACCACA 
    ACCATGAAAC ACTCCCACTC GCAGAGGATG GACTACAGGC TGCTCAAACG GCAGCTGTGC
    AGCTCCATGG CCAACTCCAG CATCCTGCCA TCTGCTGTGA CGGGGATCAG TAAGGAACTA
    GCTGACCTGC GCCACCTCAT CCAGTTTCCA GAGGAAATCG CCACCATCCT GACAGAACAG
    GAGCAGCAGC TATACCGCCG CGTCTTCCCC CTCGACTACC TCAGCTTTCT CACCCGAGAC
    CTGGGCAGCC CAGAGTGCCA CAAACGCCAC CCGCATCTAA AAGCTTCCCT GTCAGCCCCC
    ATCATGCCCA CTCAGAATGA CAACCACAAC ACTGTTGAAG ACCTCGTCAC AAGATTCAAT
    GAGGTGAGCT CATGGGTGAC CTGGCTTATC CTGACGGCGG GCTCCATGGA GGAGAAGAGG
    GAGGTTTTCT CGTACCTCGT CCATGTGGCC AAGTGCTCGT GGAACATGGG CAACTACAAC
    GCGGTCATGG AGTTCTTGGC AGGCCTCAGG TCCCGCAAAG TGCTGAAGAT GTGGCAGTTT
    ATGGATCAGG CAGATATTGA GACCATGCGA GGTCTAAAGG ATGCCATGGC CCAGCACGAA
    TCATCATCTG AGTACAAGAA AGTCGTCAGC CGTGCCCTCA ATATCCCAGG ATGCAAAGTG
    GTGCCTTTCT GTGGCGTGTT CCTCAAGGAG CTGAGTGAGG CTCTGGATGG CGCGGCCAGC
    ATTATTGGAC TGCGTCCGTC TTTTGATTCT CAAGAAGATC CTGTTGAGTT TGTCACGGAC
    TACAACGGCC AGCAGCACTT TCTGCAGAGG CTTGGAAGTG ATGGACTTCA CAGCTCAGAT
    AAAGAGGCGA CCGTCAGTAA CATACTGCAG ACCATCCGCA GCTGCAACCG AAGCCTGGAG
    GCTGAGGAGC CTGAGGAGAG GGCACGAGAG ATCACCGTTT GCCCCAAAAA TTCCTTTAAA
    GACAAGAGCA GAAACCAGTT TTCAATCGGC GACCTCTCAG ACTCTGAAGG GGACCCACTT
    GAGACAGTTA AGGATGTTGT AGACCTCCAG ACCACCGAAG ATGTTCGCGG ACCCTTTAGC
    CATGGGACTG AGCTCATTCC CTGGTACGTT CTGTCGCTTC AGCCGGATAT CCATCAGTTC
    CTGCTCCAAG GAGCAACCGT CATCCACTAC GACCCCGAGA GCCACCTCAC CGCCCGCTGT
    CTTCTCCGTC TACAGCCCGA TAACTGCTTC CTCACCTGGT GCAAACCCCA CAGTAGCTGC
    AGCCTATATG GAAGAGCTAG AACCTTCATG GGACATCCAG CATCCCCAGA CCACTTACAC
    ATCGGCCAAC CTGTTCACTG CGGACTCTCT GACGGGCTCC TGGATCTTAA TGTGGTGAAG
    GCTGTGTTTA TGGGTCACCC TGGAGTGGAC GTCAACTATG TCTGCCTGCA GCACAAACTG
    TGTAACATGA ATCCTGGTGA AAATGGTGTC ACGCTGCTAT ATGGACTTCA CACCACAGAC
    AACAGGCTCC TGCACTTTGT GGCCCCGAAA CACACGGCTC GCATGCTGCA CGAGGGCCTC
    CAGGAGCTGC TGAACTCTAT TAGGAAGATC AGGAAATTCC CTGACCAGAG ACTGCAATGG
    CTGAGAAAGC AATATGTTAG TTTGTACCAG GAGGATGGTA GATTTGAAGG ACCTACACTG
    GCTCATGCTA TCGAGCTGTT CGGAGGGAGA CGCTGGAATA TGGGAACTAG CGGCCCTGGA
    TCCGCCAGCA GAGGTGCAGA GAAGAACTCG GCTCAGAAGA ACAGTCCACT GGGCATCAAC
    TCCAATGTCA AAAAAAAGAA AAAGGCCTTA GTCAGGGGGG ACAGTGGCGA TGGAACTGAT
    GATGAGATGA CCGCACGCAA GACCAGGAGT TGCAAAGAGA CTTTGGGGAG GAGAGAGTCA
    GATGCTCTGG AAAATGTTGA GCAGGAAGAG GCTGAGGACT GCAGTACCTT TAGCCAACCT
    GGACCATTTT CCTTATCCAA CAACAAAGGC CAGTCTCCGG GATCCTCCTC GTCCTCCTCC
    ACCTCTTCTT CTGTTGCCTC CTTTTGCACA AACTCCTCCA CCCCTACACG TCCACATTCC
    TCCCCAATCC TCCCCACAAA CCTCAAGAGT CAGCCAGGGG CCTGGAGCAG TCGAAGCTGG
    CACGGTCGAG GGAAAGGCTG CTTCAGAGGT TTCCAGGACC TCATGATCTC CGACAGCATC
    ATGAGCTTTG TCGAATTTGT GGAGCTCTTC AAATCTTTTA GCATTCGCAG TCGTAAGGAT
    CTGAAGGAAC TATTCGACAC CTACGCCGTG CCCTGCAGTC GCTCTGGCCC TGAGTCTGTC
    CCCCTCTACA CCACACTTAG GATTGACGAC AAGCTAACCG GACTGCAGCC AGATCTGGAT
    CTCCTCACTC GCAACGGCTC TGACCTGGGC CTTTTCATTC GCACCCGCCA GCAAATGTCT
    GAAAACCAGA AGCAGATTTC AGACGCCATC GCAGCTGCCA GTATTGTGAC CAATGGCACT
    GGAGTGGAGA ATTCATCGCT GGGTGTGCTG GGACTGGCCA TCTCGCAGCT CAATGATTTC
    TTGGTCAACT GTCAGAGAGA ACATCTGAGC TATGATGAGA TCCTCAGCAT CATACAGAAA
    TTTGAGCCTT CTTCAAACAT GCGTCAGATG GGGTGGATGT CGTTTGAAGG ATTCGCCAGG
    TTTCTAATGG ACAAGGACAA CTTTGCCTCC AAAAATGAGG AGTCTCAAGT GAACCTGGAT
    GAGCTCCAGC ATCCGCTGTC TTACTATTAC ATCGAGTCCT CTCATAACAC ATACCTGACG
    GGGCATCAGC TGAAGGGAGA GTCTTCAGTC GAGCTCTACA GCCAGGTGTT GCTGCAGGGT
    TGTCGCAGCG TTGAGTTGGA CTGCTGGGAT GGGGATGACG GCATGCCTGT TATTTACCAT
    GGACATACTC TTACCACTAA GATTCCTTTC AAGGATGTTG TAGAGGCCAT CAACCGTGCA
    GCATTTGTGA ACTCAGAGAT GCCTGTGATT CTGTCCATTG AAAACCACTG CTCCTTACCA
    CAGCAGCGCA AAATGGCTGA GATTTTTAAG ATGGTGTTTG GTGAGAAGCT GGTGACCAAG
    TTTCTGTTTG AAAGTGATTT CGCGGACGAG CCTTTGCTTC CGTCTCCCCT TCAGCTCCGG
    GGGAAAATCC TCCTGAAGAA TAAAAAGTTA AAAGCTCACC AGGCTCCTGT TGACATTCTC
    AAACAAAAGG CGCATCAGCT TGCCCACATG CAGGCTCAGG CCAACAACGG CACAGTCAGC
    ACAACGCCAC TGGGAAACAA TGATGAAGAG GAGGAGGAGG AAGACGAGTA CGATTACGAT
    TACGAGTCGC TGTCTGACGC TGATGTCCTC AATGCTTCCA CAGCCTCTTA TGGCCAGGAA
    GACAATATTC TGGATGACAA ACCTGAAGGC AAGTCATCTA CGGAGAAACT TCAGTACGAG
    TCCAACGATG AGATGCCAAA GCGGTTTAAA AAAGCTGGAA GCAAAGGAAA GATGTTTGAC
    ATGGAGCTGG GAGAGGAGTT TTACCTGCCC CAGAACGAGA AGGAGAGTAG ACAGATTGCT
    CAAGAGCTGT CAGATCTGGT CATCTACTGC CAGGCTATCA AATTTCCAGG CCTCTCCACC
    CTCTCTCCAT CAGGCTCTGG AAGGGGGAAA GACAGAAAGA GCCGGAAATC CATCTTTGGC
    AGCGCTCCTG CTCGCGGGAG CCCCGGGGAG CCTGTGACGC TGGTCCGAGC TCCAGGGAAA
    GCCAGTCTTG AGGGCATTCG CATGAACTCG GAGGATCAAT TGTGTCTGAG CCCGAGCACC
    TCTCTGAGCT CCATCATCCG CACGCCCAAA TGCTACCACA TCTCCTCTGT GAACGAGAAT
    GCAGCGAAGC GCTTGTGTCG CCGCTACTCG CAGAAGCTGA TCCAGCACAC CAGCTGCCAG
    CTGCTGCGCA CGTACCCAGC GGCCACACGC ATTGACTCTG CCAACCCCAA TCCGCTCATC
    TTCTGGCTGC ACGGAGTCCA GCTGGTGGCC CTCAACTACC AGACTGATGA TCTGCCGATG
    CAGCTGAACG CGGCTCTATT CGAGGCTAAT GGTCACTGTG GTTTTGTGCT GAAGCCTCCA
    GTCCTGTGGG ACAGAAGCTG TCCTCTCTAT CAGCACTTTT ACCCTCTGGA CCGAGACCTG
    GAGAACATGA CCCCAACGCT ATACACACTC ACTATTGTGT CGGGGCAGAA CGTGTGTCCA
    GGAAACTCAA ACGGCAGTCC GTGTGTCGAA GTGGAGGTGT TGGGAATGCC GGCGGACAGC
    TGTCATTTCC GAACTAAACC CATACACCGC AACACACTCA ATCCCATGTG GAACGAACAC
    TTCCAGTTCC ACGTGCACTT TGAAGACCTG GCGTTCCTGC GCATCGCTGT GGTGGAGAAC
    AACAGCTCTC AGGTCACCGC TCAGAGGATA CTTCCCCTCA AAACACTCAG AGCAGGCTAC
    CGGCACCTGC AGCTGAGGAA CCTGCACAAC GAGCCTCTGG AGGTGTCCAG TCTGTTCATG
    TTCAGCCGCA GGACGGAGGA GAGCCCGACC GGAGGACCAC CGTCTGCGTC TGTGCTGTTC
    AGCACCGAGG AGAGACGATC TGTGCAGCAG CACAAGGTGA CCGTGCATGG AGTCCCCGGA
    CCTGAACCCT TCACTGTGAT CTGTGTCGAT GAATTCACCA CAGCCAAACA GCTCCTGGAC
    TCTCTGTTTC CGACTGCCTC TTTTAAGTAT ATGCTGATGG AGGAGAGAGT GTCTCTGTCG
    AAGGAGAAGA AGGCTCCTCA GCAGCGTCCT CTAGCCAATG ATGAGAGACT GCTGAAGCTC
    ATTCACAGCT GGCAGCCTGA GGACGGATAT GTGGGCAGAA TATACCTCAA AACCAGAGAA
    CAGCAGAACT GCAGTGAGAA GACGAGCGTC CCGCTGGAGT CACTGGAGGA GCTCGGCAGC
    TTAGAGGACG ACACCTTCTT CGTGCAGGTC CATGATGTTT CTCCAGAACA GCCTCACACC
    GTCATCAAAG CCCCGCGATA CAGCACAGCA CAGGACATCA TCCAGCAGAC TCTGAGCAAA
    GCCAAGTATT CTCTGAGTAT CCTGAGCAAC CCCAACCCAT GTGACTACGT CCTGATGGAG
    GAGCTTTGTA AAGACGCGGG TGGAAAGAAG TCGTCCTCTG CCAAACCGTG CCAGCGTGTG
    CTGCAGGACC ACGAGTGTGT GTTTCAGGCT CAGAGCCGCT GGAAGGGGGC TGGAAAATTC
    ATCCTCAAAC TCAAAGAGCA GTTGGCTCGT GAGGACAAGC GTAAAGGTGT GTCGTTCGCC
    AGTGAGCTGA GGAAGCTGAC GGGTCGCAGT CGCAGTGTGA CGGTCAACAC AAACAGCAAC
    GACACTCACA GTAAGGAAGA GAAGAGCGCA TGTCCCAGCA TGAGCTGTGT GCCGGAGACA
    TCGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304702.1
    CDS79..5607
    Translation

    Target ORF information:

    RefSeq Version XM_009306427.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306427.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGTGGCTGA GCTCCTCTCG CCGCTGCGGT GTGAAGCAGG GAGCCAGCGA GACCACCACA 
    ACCATGAAAC ACTCCCACTC GCAGAGGATG GACTACAGGC TGCTCAAACG GCAGCTGTGC
    AGCTCCATGG CCAACTCCAG CATCCTGCCA TCTGCTGTGA CGGGGATCAG TAAGGAACTA
    GCTGACCTGC GCCACCTCAT CCAGTTTCCA GAGGAAATCG CCACCATCCT GACAGAACAG
    GAGCAGCAGC TATACCGCCG CGTCTTCCCC CTCGACTACC TCAGCTTTCT CACCCGAGAC
    CTGGGCAGCC CAGAGTGCCA CAAACGCCAC CCGCATCTAA AAGCTTCCCT GTCAGCCCCC
    ATCATGCCCA CTCAGAATGA CAACCACAAC ACTGTTGAAG ACCTCGTCAC AAGATTCAAT
    GAGGTGAGCT CATGGGTGAC CTGGCTTATC CTGACGGCGG GCTCCATGGA GGAGAAGAGG
    GAGGTTTTCT CGTACCTCGT CCATGTGGCC AAGTGCTCGT GGAACATGGG CAACTACAAC
    GCGGTCATGG AGTTCTTGGC AGGCCTCAGG TCCCGCAAAG TGCTGAAGAT GTGGCAGTTT
    ATGGATCAGG CAGATATTGA GACCATGCGA GGTCTAAAGG ATGCCATGGC CCAGCACGAA
    TCATCATCTG AGTACAAGAA AGTCGTCAGC CGTGCCCTCA ATATCCCAGG ATGCAAAGTG
    GTGCCTTTCT GTGGCGTGTT CCTCAAGGAG CTGAGTGAGG CTCTGGATGG CGCGGCCAGC
    ATTATTGGAC TGCGTCCGTC TTTTGATTCT CAAGAAGATC CTGTTGAGTT TGTCACGGAC
    TACAACGGCC AGCAGCACTT TCTGCAGAGG CTTGGAAGTG ATGGACTTCA CAGCTCAGAT
    AAAGAGGCGA CCGTCAGTAA CATACTGCAG ACCATCCGCA GCTGCAACCG AAGCCTGGAG
    GCTGAGGAGC CTGAGGAGAG GGCACGAGAG ATCACCGTTT GCCCCAAAAA TTCCTTTAAA
    GACAAGAGCA GAAACCAGTT TTCAATCGGC GACCTCTCAG ACTCTGAAGG GGACCCACTT
    GAGACAGTTA AGGATGTTGT AGACCTCCAG ACCACCGAAG ATGTTCGCGG ACCCTTTAGC
    CATGGGACTG AGCTCATTCC CTGGTACGTT CTGTCGCTTC AGCCGGATAT CCATCAGTTC
    CTGCTCCAAG GAGCAACCGT CATCCACTAC GACCCCGAGA GCCACCTCAC CGCCCGCTGT
    CTTCTCCGTC TACAGCCCGA TAACTGCTTC CTCACCTGGT GCAAACCCCA CAGTAGCTGC
    AGCCTATATG GAAGAGCTAG AACCTTCATG GGACATCCAG CATCCCCAGA CCACTTACAC
    ATCGGCCAAC CTGTTCACTG CGGACTCTCT GACGGGCTCC TGGATCTTAA TGTGGTGAAG
    GCTGTGTTTA TGGGTCACCC TGGAGTGGAC GTCAACTATG TCTGCCTGCA GCACAAACTG
    TGTAACATGA ATCCTGGTGA AAATGGTGTC ACGCTGCTAT ATGGACTTCA CACCACAGAC
    AACAGGCTCC TGCACTTTGT GGCCCCGAAA CACACGGCTC GCATGCTGCA CGAGGGCCTC
    CAGGAGCTGC TGAACTCTAT TAGGAAGATC AGGAAATTCC CTGACCAGAG ACTGCAATGG
    CTGAGAAAGC AATATGTTAG TTTGTACCAG GAGGATGGTA GATTTGAAGG ACCTACACTG
    GCTCATGCTA TCGAGCTGTT CGGAGGGAGA CGCTGGAATA TGGGAACTAG CGGCCCTGGA
    TCCGCCAGCA GAGGTGCAGA GAAGAACTCG GCTCAGAAGA ACAGTCCACT GGGCATCAAC
    TCCAATGTCA AAAAAAAGAA AAAGGCCTTA GTCAGGGGGG ACAGTGGCGA TGGAACTGAT
    GATGAGATGA CCGCACGCAA GACCAGGAGT TGCAAAGAGA CTTTGGGGAG GAGAGAGTCA
    GATGCTCTGG AAAATGTTGA GCAGGAAGAG GCTGAGGACT GCAGTACCTT TAGCCAACCT
    GGACCATTTT CCTTATCCAA CAACAAAGGC CAGTCTCCGG GATCCTCCTC GTCCTCCTCC
    ACCTCTTCTT CTGTTGCCTC CTTTTGCACA AACTCCTCCA CCCCTACACG TCCACATTCC
    TCCCCAATCC TCCCCACAAA CCTCAAGAGT CAGCCAGGGG CCTGGAGCAG TCGAAGCTGG
    CACGGTCGAG GGAAAGGCTG CTTCAGAGGT TTCCAGGACC TCATGATCTC CGACAGCATC
    ATGAGCTTTG TCGAATTTGT GGAGCTCTTC AAATCTTTTA GCATTCGCAG TCGTAAGGAT
    CTGAAGGAAC TATTCGACAC CTACGCCGTG CCCTGCAGTC GCTCTGGCCC TGAGTCTGTC
    CCCCTCTACA CCACACTTAG GATTGACGAC AAGCTAACCG GACTGCAGCC AGATCTGGAT
    CTCCTCACTC GCAACGGCTC TGACCTGGGC CTTTTCATTC GCACCCGCCA GCAAATGTCT
    GAAAACCAGA AGCAGATTTC AGACGCCATC GCAGCTGCCA GTATTGTGAC CAATGGCACT
    GGAGTGGAGA ATTCATCGCT GGGTGTGCTG GGACTGGCCA TCTCGCAGCT CAATGATTTC
    TTGGTCAACT GTCAGAGAGA ACATCTGAGC TATGATGAGA TCCTCAGCAT CATACAGAAA
    TTTGAGCCTT CTTCAAACAT GCGTCAGATG GGGTGGATGT CGTTTGAAGG ATTCGCCAGG
    TTTCTAATGG ACAAGGACAA CTTTGCCTCC AAAAATGAGG AGTCTCAAGT GAACCTGGAT
    GAGCTCCAGC ATCCGCTGTC TTACTATTAC ATCGAGTCCT CTCATAACAC ATACCTGACG
    GGGCATCAGC TGAAGGGAGA GTCTTCAGTC GAGCTCTACA GCCAGGTGTT GCTGCAGGGT
    TGTCGCAGCG TTGAGTTGGA CTGCTGGGAT GGGGATGACG GCATGCCTGT TATTTACCAT
    GGACATACTC TTACCACTAA GATTCCTTTC AAGGATGTTG TAGAGGCCAT CAACCGTGCA
    GCATTTGTGA ACTCAGAGAT GCCTGTGATT CTGTCCATTG AAAACCACTG CTCCTTACCA
    CAGCAGCGCA AAATGGCTGA GATTTTTAAG ATGGTGTTTG GTGAGAAGCT GGTGACCAAG
    TTTCTGTTTG AAAGTGATTT CGCGGACGAG CCTTTGCTTC CGTCTCCCCT TCAGCTCCGG
    GGGAAAATCC TCCTGAAGAA TAAAAAGTTA AAAGCTCACC AGGCTCCTGT TGACATTCTC
    AAACAAAAGG CGCATCAGCT TGCCCACATG CAGGCTCAGG CCAACAACGG CACAGTCAGC
    ACAACGCCAC TGGGAAACAA TGATGAAGAG GAGGAGGAGG AAGACGAGTA CGATTACGAT
    TACGAGTCGC TGTCTGACGC TGATGTCCTC AATGCTTCCA CAGCCTCTTA TGGCCAGGAA
    GACAATATTC TGGATGACAA ACCTGAAGGC AAGTCATCTA CGGAGAAACT TCAGTACGAG
    TCCAACGATG AGATGCCAAA GCGGTTTAAA AAAGCTGGAA GCAAAGGAAA GATGTTTGAC
    ATGGAGCTGG GAGAGGAGTT TTACCTGCCC CAGAACGAGA AGGAGAGTAG ACAGATTGCT
    CAAGAGCTGT CAGATCTGGT CATCTACTGC CAGGCTATCA AATTTCCAGG CCTCTCCACC
    CTCTCTCCAT CAGGCTCTGG AAGGGGGAAA GACAGAAAGA GCCGGAAATC CATCTTTGGC
    AGCGCTCCTG CTCGCGGGAG CCCCGGGGAG CCTGTGACGC TGGTCCGAGC TCCAGGGAAA
    GCCAGTCTTG AGGGCATTCG CATGAACTCG GAGGATCAAT TGTGTCTGAG CCCGAGCACC
    TCTCTGAGCT CCATCATCCG CACGCCCAAA TGCTACCACA TCTCCTCTGT GAACGAGAAT
    GCAGCGAAGC GCTTGTGTCG CCGCTACTCG CAGAAGCTGA TCCAGCACAC CAGCTGCCAG
    CTGCTGCGCA CGTACCCAGC GGCCACACGC ATTGACTCTG CCAACCCCAA TCCGCTCATC
    TTCTGGCTGC ACGGAGTCCA GCTGGTGGCC CTCAACTACC AGACTGATGA TCTGCCGATG
    CAGCTGAACG CGGCTCTATT CGAGGCTAAT GGTCACTGTG GTTTTGTGCT GAAGCCTCCA
    GTCCTGTGGG ACAGAAGCTG TCCTCTCTAT CAGCACTTTT ACCCTCTGGA CCGAGACCTG
    GAGAACATGA CCCCAACGCT ATACACACTC ACTATTGTGT CGGGGCAGAA CGTGTGTCCA
    GGAAACTCAA ACGGCAGTCC GTGTGTCGAA GTGGAGGTGT TGGGAATGCC GGCGGACAGC
    TGTCATTTCC GAACTAAACC CATACACCGC AACACACTCA ATCCCATGTG GAACGAACAC
    TTCCAGTTCC ACGTGCACTT TGAAGACCTG GCGTTCCTGC GCATCGCTGT GGTGGAGAAC
    AACAGCTCTC AGGTCACCGC TCAGAGGATA CTTCCCCTCA AAACACTCAG AGCAGGCTAC
    CGGCACCTGC AGCTGAGGAA CCTGCACAAC GAGCCTCTGG AGGTGTCCAG TCTGTTCATG
    TTCAGCCGCA GGACGGAGGA GAGCCCGACC GGAGGACCAC CGTCTGCGTC TGTGCTGTTC
    AGCACCGAGG AGAGACGATC TGTGCAGCAG CACAAGGTGA CCGTGCATGG AGTCCCCGGA
    CCTGAACCCT TCACTGTGAT CTGTGTCGAT GAATTCACCA CAGCCAAACA GCTCCTGGAC
    TCTCTGTTTC CGACTGCCTC TTTTAAGTAT ATGCTGATGG AGGAGAGAGT GTCTCTGTCG
    AAGGAGAAGA AGGCTCCTCA GCAGCGTCCT CTAGCCAATG ATGAGAGACT GCTGAAGCTC
    ATTCACAGCT GGCAGCCTGA GGACGGATAT GTGGGCAGAA TATACCTCAA AACCAGAGAA
    CAGCAGAACT GCAGTGAGAA GACGAGCGTC CCGCTGGAGT CACTGGAGGA GCTCGGCAGC
    TTAGAGGACG ACACCTTCTT CGTGCAGGTC CATGATGTTT CTCCAGAACA GCCTCACACC
    GTCATCAAAG CCCCGCGATA CAGCACAGCA CAGGACATCA TCCAGCAGAC TCTGAGCAAA
    GCCAAGTATT CTCTGAGTAT CCTGAGCAAC CCCAACCCAT GTGACTACGT CCTGATGGAG
    GAGCTTTGTA AAGACGCGGG TGGAAAGAAG TCGTCCTCTG CCAAACCGTG CCAGCGTGTG
    CTGCAGGACC ACGAGTGTGT GTTTCAGGCT CAGAGCCGCT GGAAGGGGGC TGGAAAATTC
    ATCCTCAAAC TCAAAGAGCA GTTGGCTCGT GAGGACAAGC GTAAAGGTGT GTCGTTCGCC
    AGTGAGCTGA GGAAGCTGAC GGGTCGCAGT CGCAGTGTGA CGGTCAACAC AAACAGCAAC
    GACACTCACA GTAAGGAAGA GAAGAGCGCA TGTCCCAGCA TGAGCTGTGT GCCGGAGACA
    TCGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa35532
    Clone ID Related Accession (Same CDS sequence) NM_001161653.1
    Accession Version NM_001161653.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6747bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU919685.1. On Jun 10, 2009 this sequence version replaced XM_001921507.1. ##Evidence-Data-START## Transcript exon combination :: EU919685.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705, SAMN06350654 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTGCTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGCGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCWAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTGTGT GTGCCATATG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAGGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GTGGCCACAG CTGCTCTTGG GGCCCGCACT TCTCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCCG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCGCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACAGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGACC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCAGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAA
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCCGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GAAAGGACCT
    GGGACTGAAC GAGTCCTGTT TTCAATCGGC GACCTCTCAG ACTCTGAAGG GGACCCACTT
    GAGACAGTTA AGGATGTTGT AGACCTCCAG ACCACCGAAG ATGTTCGCGG ACCCTTTAGC
    CATGGGACAG AGCTCATTCC CTGGTACGTT CTGTCGCTTC AGCCGGATAT CCATCAGTTC
    CTGCTCCAAG GAGCAACCGT CATCCACTAC GACCCCGAGA GCCACCTCAC CGCCCGATGT
    CTTCTCCGTC TACAGCCCGA TAACTGCTTC CTCACCTGGT GCAAACCCCA CAGTAGCTGC
    AGCCTATATG GAAGAGCTAG AACCTTCATG GGACATCCAG CATCCCCAGA CCACTTACAC
    ATCGGCCAAC CTGTTCACTG TGGACTCTCT GACGGGCTCC TGGATCTTAA TGTGGTGAAG
    GCTGTGTTTA TGGGTCACCC TGGAGTGGAC GTCAACTATG TCTGCCTGCA GCACAAACTG
    TGTAACATGA ATCCTGGTGG AAATGGTGCC ACGCTGCTAT ATGGACTTCA CACCACAGAC
    AACAGGCTCC TGCACTTTGT GGCCCCGAAA CACACGGCTC GCATGCTGCA CGAGGGCCTC
    CAGGAGCTGC TGAACTCTAT CAGGAAGATC AGGAAATTCC CTGACCAGAG ACTGCAATGG
    CTGAGAAAGC AATATGTTAG TTTGTACCAG GAGGATGGTA GATTTGAAGG ACCTACACTG
    GCTCATGCTA TCGAGCTGTT CGGAGGGAGA CGCTGGAATA TGGGAACTAG CGGCCCAGGA
    TCCGCCAGCA GAAGTGCAGA GAAGAACTCG GCTCAGAAAA ACAGTCCACT GGGCATCAAC
    TCCAATGTCA AAAAGAAGAA AAAGGCCTTA GTCAGGGGGG ACAGTGGCGA TGGAACTGAT
    GATGAGATGA CCGCACGCAA GACCAGGGGT TGCAAAGAGA CTTTGGGGAG GAGAGAGTCA
    GATCCTCTGG AAAATGTTGA GCAGGGAGAG GCTGAGGACT GCAGTACCTT TAGCCAACCT
    GGACCATCTT CCTTATCCAA CAACAAAGGC CAGTCTCCGG GATCCTCCTC GTCCTCCTCC
    ACCTCTTCTT CTGTTGCCTC CTTTTGCACA AACTCCTCCA CCCCTACACG TCCACATTCC
    TCCCCGATCC TCCCCACAAA CCTCAAGAGT CAGCCAGGGG CCTGGAGCAG TCGAAGCTGG
    CACGGTCGAG GGAAAGGCTG CTTCAGAGGT TTCCAGGACC TCATGATCTC CGACAGCATC
    ATGAGCTTTG TCGAATTTGT GGAGCTCTTC AAATCTTTTA GCATTCGCAG TCGTAAGGAT
    CTGAAGGAAC TATTCGACAC CTACGCCGTG CCCTGCAGTC GCTCTGGCCC TGAGTCTGTC
    CCCCTCTACA CCACACTTAG GATTGACGAC AAGCTAACCG GACTGCAGCC AGATCTGGAT
    CTCCTCACTC GCAACGGCTC TGACCTGGGC CTTTTCATTC GCACCCGCCA GCAAATGTCT
    GAAAACCAGA AGCAGATTTC AGACGCCATC GCAGCTGCCA GTATTGTGAC CAATGGCACT
    GGAGTGGAGA ATTCATCGCT GGGTGTGCTG GGACTGGCCA TCTCGCAGCT CAATGATTTC
    TTGGTCAACT GTCAGAGAGA ACATCTGAGC TATGATGAGA TCCTCAGCAT CATACAGAAA
    TTTGAGCCTT CTTCAAACAT GCGTCAGATG GGGTGGATGT CGTTTGAAGG ATTCGCCAGG
    TTTCTAATGG ACAAGGACAA CTTTGCCTCC AAAAATGAGG AGTCTCAAGT GAACCTGGAT
    GAGCTCCAGC ATCCGCTGTC TTACTATTAC ATCGAGTCCT CTCATAACAC ATACCTGACG
    GGGCATCAGC TGAAGGGAGA GTCTTCAGTC GAGCTCTACA GCCAGGTGTT GCTGCAGGGT
    TGTCGCAGCG TTGAGTTGGA CTGCTGGGAT GGGGATGACG GCATGCCTGT TATTTACCAT
    GGACATACTC TTACCACTAA GATTCCTTTC AAGGATGTTG TAGAGGCCAT CAACCGTGCA
    GCATTTGTGA ACTCAGAGAT GCCTGTGATT CTGTCCATTG AAAACCACTG CTCCTTACCA
    CAGCAGCGCA AAATGGCTGA GATTTTTAAG ATGGTGTTTG GTGAGAAGCT GGTGACCAAG
    TTTYTGTTTG AAAGYGATTT CGCGGACGAG CCTTTGCTTC CGTCTCCCCT TCAGCTCCGG
    GGGAAAATCC TCCTGAAGAA TAAAAAGTTA AAAGCTCACC AGGCTCCTGT TGACATTCTC
    AAACAAAAGG CGCATCAGCT TGCCCACATG CAGGCTCAGG CCAACAACGG CACAGTCAGC
    ACAACGCCAC TGGGAAACAA TGATGAAGAG GAGGAGGAGG AAGACGAGTA CGATTACGAT
    TACGAGTCGC TGTCTGACGA CAATATTCTG GATGACAAAC CTGAAGGCAA GTCATCTACG
    GAGAAACTTC AGTACGAGTC CAACGATGAG ATGCCAAAGC GGTTTAAAAA AGCTGGAAGC
    AAAGGAAAGA TGTTTGACAT GGAGCTGGGA GAGGAGTTTT ACCTGCCCCA GAACGAGAAG
    GAGAGTAGAC AGATTGCTCA AGAGCTGTCA GATCTGGTCA TCTACTGCCA GGCCATTAAA
    TTTCCAGGCC TCTCCACCCT CTCTCCATCA GGCTCTGGAA GGGGGAAAGA CAGAAAGAGC
    CGGAAATCCA TCTTTGGCAG CGCTCCTGCT CGCGGGAGCC CCGGGGAGCC TGTGACGCTG
    GTCCGAGCTC CAGGGAAAGC CAGTCTTGAG GGCATTCGCA TGAACTCGGA GGAGCAAATG
    TGTCTGAGCC CGAGCACCTC TCTGAGCTCC ATCATCCGCA CGCCCAAATG CTACCACATC
    TCCTCTGTGA ACGAGAATGC AGCGAAGCGC TTGTGTCGCC GCTACTCGCA GAAGCTCATC
    CAGCACACCA GCTGCCAGCT GCTGCGCACG TACCCGGCGG CCACACGCAT TGACTCTGCC
    AACCCCAATC CGCTCATCTT CTGGCTGCAC GGAGTCCAGC TGGTGGCCCT CAACTACCAG
    ACTGATGATC TGCCGATGCA GCTGAACGCG GCTCTATTCG AGGCTAATGG TCACTGTGGT
    TTTGTGCTGA AGCCTCCAGT CCTGTGGGAC AGAAGCTGTC CTCTCTATCA GCACTTTTAC
    CCTCTGGACC GAGACCTGGA GAACATGACC CCAACGCTAT ACACACTCAC TATTGTGTCG
    GGGCAGAACG TGTGTCCAGG AAACTCAAAC GGCAGTCCGT GTGTCGAAGT GGAGGTGTTG
    GGAATGCCGG CGGACAGCTG TCATTTCCGA ACTAAACCCA TACACCGCAA CACACTCAAT
    CCCATGTGGA ACGAACACTT CCAGTTCCAC GTGCACTTTG AAGACCTGGC ATTCCTGCGC
    ATCGCTGTGG TGGAGAACAA CAGCTCTCAG GTCACCGCTC AGAGGATACT TCCCCTCAAA
    ACACTCAGAG CAGGCTACCG GCACCTGCAG CTGAGGAACC TGCACAACGA GCCTCTGGAG
    GTGTCCAGTC TGTTCATGTT CAGCCGCAGG ACGGAGGAGA GCCCGACTGG AGGACCACCG
    TCTGCGTCTG TGCTGTTCAG CACCGAGGAG AGACGATCTG TGCAGCAGCA CAAGGTGACC
    GTGCATGGAG TCCCCGGACC TGAACCCTTC ACTGTGATCT GTGTCGATGA ATTCACCACA
    GCCAAACAGC TCCTGGACTC TCTGTTTCCG ACTGCCTCCT TTAAGTATAT GCTGATGGAG
    GAGAGAGTGT CTCTGTCGAA GGAGAAGAAG GCTCCTCAGC AGCGTCCTTT AGCCAATGAT
    GAGAGACTTC TGAAGCTCAT TCACAGCTGG CAGCCTGAGG ACGGATATGT GGGCAGAATA
    TACCTCAAAA CCAGAGAACA GCAGAACTGC AGTGAGAAGA CGAGCGTCCC GCTGGAGTCA
    CTGGAGGAGC TCGGCAGCTC AGAGGACGAC ACCTTCTTCG TGCAGGTCCA TGATGTTTCT
    CCAGAACAGC CTCACACCGT CATCAAAGCC CCGCGATACA GCACAGCACA GGACATCATC
    CAGCAGACTC TGAGCAAAGC CAAGTATTCT CTGAGTATCC TGAGCAACCC CAACCCATGT
    GACTACGTCC TGATGGAGGA GCTTTGTAAA GACGCGGGTG GAAAGAAGTC GTCCTCTGCC
    AAACCGTGCC AGCGTGTGCT GCAGGACCAC GAGTGTGTGT TTCAGGCTCA GAGCCGCTGG
    AAGGGGGCTG GAAAATTCAT CCTCAAACTC AAAGAGCAGT TGGCTCGTGA GGACAAGCGT
    AAAGGTGTGT CGTTCGCCAG TGAGCTGAGG AAGCTGACGG GTCGCAGTCG CAGTGTGACG
    GTCAACACAA ACAGCAACGA CACTCACAGT AAGGAAGAGA AGAGCGCATG TCCCAGCATG
    AGCTGTGTGC CGGAGACATC GCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001155125.1
    CDS1..6747
    Translation

    Target ORF information:

    RefSeq Version NM_001161653.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, epsilon 1 (plce1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001161653.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTGCTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGCGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCWAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTGTGT GTGCCATATG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAGGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GTGGCCACAG CTGCTCTTGG GGCCCGCACT TCTCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCCG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCGCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACAGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGACC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCAGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAA
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCCGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GAAAGGACCT
    GGGACTGAAC GAGTCCTGTT TTCAATCGGC GACCTCTCAG ACTCTGAAGG GGACCCACTT
    GAGACAGTTA AGGATGTTGT AGACCTCCAG ACCACCGAAG ATGTTCGCGG ACCCTTTAGC
    CATGGGACAG AGCTCATTCC CTGGTACGTT CTGTCGCTTC AGCCGGATAT CCATCAGTTC
    CTGCTCCAAG GAGCAACCGT CATCCACTAC GACCCCGAGA GCCACCTCAC CGCCCGATGT
    CTTCTCCGTC TACAGCCCGA TAACTGCTTC CTCACCTGGT GCAAACCCCA CAGTAGCTGC
    AGCCTATATG GAAGAGCTAG AACCTTCATG GGACATCCAG CATCCCCAGA CCACTTACAC
    ATCGGCCAAC CTGTTCACTG TGGACTCTCT GACGGGCTCC TGGATCTTAA TGTGGTGAAG
    GCTGTGTTTA TGGGTCACCC TGGAGTGGAC GTCAACTATG TCTGCCTGCA GCACAAACTG
    TGTAACATGA ATCCTGGTGG AAATGGTGCC ACGCTGCTAT ATGGACTTCA CACCACAGAC
    AACAGGCTCC TGCACTTTGT GGCCCCGAAA CACACGGCTC GCATGCTGCA CGAGGGCCTC
    CAGGAGCTGC TGAACTCTAT CAGGAAGATC AGGAAATTCC CTGACCAGAG ACTGCAATGG
    CTGAGAAAGC AATATGTTAG TTTGTACCAG GAGGATGGTA GATTTGAAGG ACCTACACTG
    GCTCATGCTA TCGAGCTGTT CGGAGGGAGA CGCTGGAATA TGGGAACTAG CGGCCCAGGA
    TCCGCCAGCA GAAGTGCAGA GAAGAACTCG GCTCAGAAAA ACAGTCCACT GGGCATCAAC
    TCCAATGTCA AAAAGAAGAA AAAGGCCTTA GTCAGGGGGG ACAGTGGCGA TGGAACTGAT
    GATGAGATGA CCGCACGCAA GACCAGGGGT TGCAAAGAGA CTTTGGGGAG GAGAGAGTCA
    GATCCTCTGG AAAATGTTGA GCAGGGAGAG GCTGAGGACT GCAGTACCTT TAGCCAACCT
    GGACCATCTT CCTTATCCAA CAACAAAGGC CAGTCTCCGG GATCCTCCTC GTCCTCCTCC
    ACCTCTTCTT CTGTTGCCTC CTTTTGCACA AACTCCTCCA CCCCTACACG TCCACATTCC
    TCCCCGATCC TCCCCACAAA CCTCAAGAGT CAGCCAGGGG CCTGGAGCAG TCGAAGCTGG
    CACGGTCGAG GGAAAGGCTG CTTCAGAGGT TTCCAGGACC TCATGATCTC CGACAGCATC
    ATGAGCTTTG TCGAATTTGT GGAGCTCTTC AAATCTTTTA GCATTCGCAG TCGTAAGGAT
    CTGAAGGAAC TATTCGACAC CTACGCCGTG CCCTGCAGTC GCTCTGGCCC TGAGTCTGTC
    CCCCTCTACA CCACACTTAG GATTGACGAC AAGCTAACCG GACTGCAGCC AGATCTGGAT
    CTCCTCACTC GCAACGGCTC TGACCTGGGC CTTTTCATTC GCACCCGCCA GCAAATGTCT
    GAAAACCAGA AGCAGATTTC AGACGCCATC GCAGCTGCCA GTATTGTGAC CAATGGCACT
    GGAGTGGAGA ATTCATCGCT GGGTGTGCTG GGACTGGCCA TCTCGCAGCT CAATGATTTC
    TTGGTCAACT GTCAGAGAGA ACATCTGAGC TATGATGAGA TCCTCAGCAT CATACAGAAA
    TTTGAGCCTT CTTCAAACAT GCGTCAGATG GGGTGGATGT CGTTTGAAGG ATTCGCCAGG
    TTTCTAATGG ACAAGGACAA CTTTGCCTCC AAAAATGAGG AGTCTCAAGT GAACCTGGAT
    GAGCTCCAGC ATCCGCTGTC TTACTATTAC ATCGAGTCCT CTCATAACAC ATACCTGACG
    GGGCATCAGC TGAAGGGAGA GTCTTCAGTC GAGCTCTACA GCCAGGTGTT GCTGCAGGGT
    TGTCGCAGCG TTGAGTTGGA CTGCTGGGAT GGGGATGACG GCATGCCTGT TATTTACCAT
    GGACATACTC TTACCACTAA GATTCCTTTC AAGGATGTTG TAGAGGCCAT CAACCGTGCA
    GCATTTGTGA ACTCAGAGAT GCCTGTGATT CTGTCCATTG AAAACCACTG CTCCTTACCA
    CAGCAGCGCA AAATGGCTGA GATTTTTAAG ATGGTGTTTG GTGAGAAGCT GGTGACCAAG
    TTTYTGTTTG AAAGYGATTT CGCGGACGAG CCTTTGCTTC CGTCTCCCCT TCAGCTCCGG
    GGGAAAATCC TCCTGAAGAA TAAAAAGTTA AAAGCTCACC AGGCTCCTGT TGACATTCTC
    AAACAAAAGG CGCATCAGCT TGCCCACATG CAGGCTCAGG CCAACAACGG CACAGTCAGC
    ACAACGCCAC TGGGAAACAA TGATGAAGAG GAGGAGGAGG AAGACGAGTA CGATTACGAT
    TACGAGTCGC TGTCTGACGA CAATATTCTG GATGACAAAC CTGAAGGCAA GTCATCTACG
    GAGAAACTTC AGTACGAGTC CAACGATGAG ATGCCAAAGC GGTTTAAAAA AGCTGGAAGC
    AAAGGAAAGA TGTTTGACAT GGAGCTGGGA GAGGAGTTTT ACCTGCCCCA GAACGAGAAG
    GAGAGTAGAC AGATTGCTCA AGAGCTGTCA GATCTGGTCA TCTACTGCCA GGCCATTAAA
    TTTCCAGGCC TCTCCACCCT CTCTCCATCA GGCTCTGGAA GGGGGAAAGA CAGAAAGAGC
    CGGAAATCCA TCTTTGGCAG CGCTCCTGCT CGCGGGAGCC CCGGGGAGCC TGTGACGCTG
    GTCCGAGCTC CAGGGAAAGC CAGTCTTGAG GGCATTCGCA TGAACTCGGA GGAGCAAATG
    TGTCTGAGCC CGAGCACCTC TCTGAGCTCC ATCATCCGCA CGCCCAAATG CTACCACATC
    TCCTCTGTGA ACGAGAATGC AGCGAAGCGC TTGTGTCGCC GCTACTCGCA GAAGCTCATC
    CAGCACACCA GCTGCCAGCT GCTGCGCACG TACCCGGCGG CCACACGCAT TGACTCTGCC
    AACCCCAATC CGCTCATCTT CTGGCTGCAC GGAGTCCAGC TGGTGGCCCT CAACTACCAG
    ACTGATGATC TGCCGATGCA GCTGAACGCG GCTCTATTCG AGGCTAATGG TCACTGTGGT
    TTTGTGCTGA AGCCTCCAGT CCTGTGGGAC AGAAGCTGTC CTCTCTATCA GCACTTTTAC
    CCTCTGGACC GAGACCTGGA GAACATGACC CCAACGCTAT ACACACTCAC TATTGTGTCG
    GGGCAGAACG TGTGTCCAGG AAACTCAAAC GGCAGTCCGT GTGTCGAAGT GGAGGTGTTG
    GGAATGCCGG CGGACAGCTG TCATTTCCGA ACTAAACCCA TACACCGCAA CACACTCAAT
    CCCATGTGGA ACGAACACTT CCAGTTCCAC GTGCACTTTG AAGACCTGGC ATTCCTGCGC
    ATCGCTGTGG TGGAGAACAA CAGCTCTCAG GTCACCGCTC AGAGGATACT TCCCCTCAAA
    ACACTCAGAG CAGGCTACCG GCACCTGCAG CTGAGGAACC TGCACAACGA GCCTCTGGAG
    GTGTCCAGTC TGTTCATGTT CAGCCGCAGG ACGGAGGAGA GCCCGACTGG AGGACCACCG
    TCTGCGTCTG TGCTGTTCAG CACCGAGGAG AGACGATCTG TGCAGCAGCA CAAGGTGACC
    GTGCATGGAG TCCCCGGACC TGAACCCTTC ACTGTGATCT GTGTCGATGA ATTCACCACA
    GCCAAACAGC TCCTGGACTC TCTGTTTCCG ACTGCCTCCT TTAAGTATAT GCTGATGGAG
    GAGAGAGTGT CTCTGTCGAA GGAGAAGAAG GCTCCTCAGC AGCGTCCTTT AGCCAATGAT
    GAGAGACTTC TGAAGCTCAT TCACAGCTGG CAGCCTGAGG ACGGATATGT GGGCAGAATA
    TACCTCAAAA CCAGAGAACA GCAGAACTGC AGTGAGAAGA CGAGCGTCCC GCTGGAGTCA
    CTGGAGGAGC TCGGCAGCTC AGAGGACGAC ACCTTCTTCG TGCAGGTCCA TGATGTTTCT
    CCAGAACAGC CTCACACCGT CATCAAAGCC CCGCGATACA GCACAGCACA GGACATCATC
    CAGCAGACTC TGAGCAAAGC CAAGTATTCT CTGAGTATCC TGAGCAACCC CAACCCATGT
    GACTACGTCC TGATGGAGGA GCTTTGTAAA GACGCGGGTG GAAAGAAGTC GTCCTCTGCC
    AAACCGTGCC AGCGTGTGCT GCAGGACCAC GAGTGTGTGT TTCAGGCTCA GAGCCGCTGG
    AAGGGGGCTG GAAAATTCAT CCTCAAACTC AAAGAGCAGT TGGCTCGTGA GGACAAGCGT
    AAAGGTGTGT CGTTCGCCAG TGAGCTGAGG AAGCTGACGG GTCGCAGTCG CAGTGTGACG
    GTCAACACAA ACAGCAACGA CACTCACAGT AAGGAAGAGA AGAGCGCATG TCCCAGCATG
    AGCTGTGTGC CGGAGACATC GCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148220
    Clone ID Related Accession (Same CDS sequence) XM_021480147.1
    Accession Version XM_021480147.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GAAAGGACCT
    GGGACTGAAC GAGTCCTGTT TTCAATCGGC GACCTCTCAG ACTCTGAAGG GGACCCACTT
    GAGACAGTTA AGGATGTTGT AGACCTCCAG ACCACCGAAG ATGTTCGCGG ACCCTTTAGC
    CATGGGACTG AGCTCATTCC CTGGTACGTT CTGTCGCTTC AGCCGGATAT CCATCAGTTC
    CTGCTCCAAG GAGCAACCGT CATCCACTAC GACCCCGAGA GCCACCTCAC CGCCCGCTGT
    CTTCTCCGTC TACAGCCCGA TAACTGCTTC CTCACCTGGT GCAAACCCCA CAGTAGCTGC
    AGCCTATATG GAAGAGCTAG AACCTTCATG GGACATCCAG CATCCCCAGA CCACTTACAC
    ATCGGCCAAC CTGTTCACTG CGGACTCTCT GACGGGCTCC TGGATCTTAA TGTGGTGAAG
    GCTGTGTTTA TGGGTCACCC TGGAGTGGAC GTCAACTATG TCTGCCTGCA GCACAAACTG
    TGTAACATGA ATCCTGGTGA AAATGGTGTC ACGCTGCTAT ATGGACTTCA CACCACAGAC
    AACAGGCTCC TGCACTTTGT GGCCCCGAAA CACACGGCTC GCATGCTGCA CGAGGGCCTC
    CAGGAGCTGC TGAACTCTAT TAGGAAGATC AGGAAATTCC CTGACCAGAG ACTGCAATGG
    CTGAGAAAGC AATATGTTAG TTTGTACCAG GAGGATGGTA GATTTGAAGG ACCTACACTG
    GCTCATGCTA TCGAGCTGTT CGGAGGGAGA CGCTGGAATA TGGGAACTAG CGGCCCTGGA
    TCCGCCAGCA GAGGTGCAGA GAAGAACTCG GCTCAGAAGA ACAGTCCACT GGGCATCAAC
    TCCAATGTCA AAAAAAAGAA AAAGGCCTTA GTCAGGGGGG ACAGTGGCGA TGGAACTGAT
    GATGAGATGA CCGCACGCAA GACCAGGAGT TGCAAAGAGA CTTTGGGGAG GAGAGAGTCA
    GATGCTCTGG AAAATGTTGA GCAGGAAGAG GCTGAGGACT GCAGTACCTT TAGCCAACCT
    GGACCATTTT CCTTATCCAA CAACAAAGGC CAGTCTCCGG GATCCTCCTC GTCCTCCTCC
    ACCTCTTCTT CTGTTGCCTC CTTTTGCACA AACTCCTCCA CCCCTACACG TCCACATTCC
    TCCCCAATCC TCCCCACAAA CCTCAAGAGT CAGCCAGGGG CCTGGAGCAG TCGAAGCTGG
    CACGGTCGAG GGAAAGGCTG CTTCAGAGGT TTCCAGGACC TCATGATCTC CGACAGCATC
    ATGAGCTTTG TCGAATTTGT GGAGCTCTTC AAATCTTTTA GCATTCGCAG TCGTAAGGAT
    CTGAAGGAAC TATTCGACAC CTACGCCGTG CCCTGCAGTC GCTCTGGCCC TGAGTCTGTC
    CCCCTCTACA CCACACTTAG GATTGACGAC AAGCTAACCG GACTGCAGCC AGATCTGGAT
    CTCCTCACTC GCAACGGCTC TGACCTGGGC CTTTTCATTC GCACCCGCCA GCAAATGTCT
    GAAAACCAGA AGCAGATTTC AGACGCCATC GCAGCTGCCA GTATTGTGAC CAATGGCACT
    GGAGTGGAGA ATTCATCGCT GGGTGTGCTG GGACTGGCCA TCTCGCAGCT CAATGATTTC
    TTGGTCAACT GTCAGAGAGA ACATCTGAGC TATGATGAGA TCCTCAGCAT CATACAGAAA
    TTTGAGCCTT CTTCAAACAT GCGTCAGATG GGGTGGATGT CGTTTGAAGG ATTCGCCAGG
    TTTCTAATGG ACAAGGACAA CTTTGCCTCC AAAAATGAGG AGTCTCAAGT GAACCTGGAT
    GAGCTCCAGC ATCCGCTGTC TTACTATTAC ATCGAGTCCT CTCATAACAC ATACCTGACG
    GGGCATCAGC TGAAGGGAGA GTCTTCAGTC GAGCTCTACA GCCAGGTGTT GCTGCAGGGT
    TGTCGCAGCG TTGAGTTGGA CTGCTGGGAT GGGGATGACG GCATGCCTGT TATTTACCAT
    GGACATACTC TTACCACTAA GATTCCTTTC AAGGATGTTG TAGAGGCCAT CAACCGTGCA
    GCATTTGTGA ACTCAGAGAT GCCTGTGATT CTGTCCATTG AAAACCACTG CTCCTTACCA
    CAGCAGCGCA AAATGGCTGA GATTTTTAAG ATGGTGTTTG GTGAGAAGCT GGTGACCAAG
    TTTCTGTTTG AAAGTGATTT CGCGGACGAG CCTTTGCTTC CGTCTCCCCT TCAGCTCCGG
    GGGAAAATCC TCCTGAAGAA TAAAAAGTTA AAAGCTCACC AGGCTCCTGT TGACATTCTC
    AAACAAAAGG CGCATCAGCT TGCCCACATG CAGGCTCAGG CCAACAACGG CACAGTCAGC
    ACAACGCCAC TGGGAAACAA TGATGAAGAG GAGGAGGAGG AAGACGAGTA CGATTACGAT
    TACGAGTCGC TGTCTGACGC TGATGTCCTC AATGCTTCCA CAGCCTCTTA TGGCCAGGAA
    GACAATATTC TGGATGACAA ACCTGAAGGC AAGTCATCTA CGGAGAAACT TCAGTACGAG
    TCCAACGATG AGATGCCAAA GCGGTTTAAA AAAGCTGGAA GCAAAGGAAA GATGTTTGAC
    ATGGAGCTGG GAGAGGAGTT TTACCTGCCC CAGAACGAGA AGGAGAGTAG ACAGATTGCT
    CAAGAGCTGT CAGATCTGGT CATCTACTGC CAGGCTATCA AATTTCCAGG CCTCTCCACC
    CTCTCTCCAT CAGGCTCTGG AAGGGGGAAA GACAGAAAGA GCCGGAAATC CATCTTTGGC
    AGCGCTCCTG CTCGCGGGAG CCCCGGGGAG CCTGTGACGC TGGTCCGAGC TCCAGGGAAA
    GCCAGTCTTG AGGGCATTCG CATGAACTCG GAGGATCAAT TGTGTCTGAG CCCGAGCACC
    TCTCTGAGCT CCATCATCCG CACGCCCAAA TGCTACCACA TCTCCTCTGT GAACGAGAAT
    GCAGCGAAGC GCTTGTGTCG CCGCTACTCG CAGAAGCTGA TCCAGCACAC CAGCTGCCAG
    CTGCTGCGCA CGTACCCAGC GGCCACACGC ATTGACTCTG CCAACCCCAA TCCGCTCATC
    TTCTGGCTGC ACGGAGTCCA GCTGGTGGCC CTCAACTACC AGACTGATGA TCTGCCGATG
    CAGCTGAACG CGGCTCTATT CGAGGCTAAT GGTCACTGTG GTTTTGTGCT GAAGCCTCCA
    GTCCTGTGGG ACAGAAGCTG TCCTCTCTAT CAGCACTTTT ACCCTCTGGA CCGAGACCTG
    GAGAACATGA CCCCAACGCT ATACACACTC ACTATTGTGT CGGGGCAGAA CGTGTGTCCA
    GGAAACTCAA ACGGCAGTCC GTGTGTCGAA GTGGAGGTGT TGGGAATGCC GGCGGACAGC
    TGTCATTTCC GAACTAAACC CATACACCGC AACACACTCA ATCCCATGTG GAACGAACAC
    TTCCAGTTCC ACGTGCACTT TGAAGACCTG GCGTTCCTGC GCATCGCTGT GGTGGAGAAC
    AACAGCTCTC AGGTCACCGC TCAGAGGATA CTTCCCCTCA AAACACTCAG AGCAGGCTAC
    CGGCACCTGC AGCTGAGGAA CCTGCACAAC GAGCCTCTGG AGGTGTCCAG TCTGTTCATG
    TTCAGCCGCA GGACGGAGGA GAGCCCGACC GGAGGACCAC CGTCTGCGTC TGTGCTGTTC
    AGCACCGAGG AGAGACGATC TGTGCAGCAG CACAAGGTGA CCGTGCATGG AGTCCCCGGA
    CCTGAACCCT TCACTGTGAT CTGTGTCGAT GAATTCACCA CAGCCAAACA GCTCCTGGAC
    TCTCTGTTTC CGACTGCCTC TTTTAAGTAT ATGCTGATGG AGGAGAGAGT GTCTCTGTCG
    AAGGAGAAGA AGGCTCCTCA GCAGCGTCCT CTAGCCAATG ATGAGAGACT GCTGAAGCTC
    ATTCACAGCT GGCAGCCTGA GGACGGATAT GTGGGCAGAA TATACCTCAA AACCAGAGAA
    CAGCAGAACT GCAGTGAGAA GACGAGCGTC CCGCTGGAGT CACTGGAGGA GCTCGGCAGC
    TTAGAGGACG ACACCTTCTT CGTGCAGGTC CATGATGTTT CTCCAGAACA GCCTCACACC
    GTCATCAAAG CCCCGCGATA CAGCACAGCA CAGGACATCA TCCAGCAGAC TCTGAGCAAA
    GCCAAGTATT CTCTGAGTAT CCTGAGCAAC CCCAACCCAT GTGACTACGT CCTGATGGAG
    GAGCTTTGTA AAGACGCGGG TGGAAAGAAG TCGTCCTCTG CCAAACCGTG CCAGCGTGTG
    CTGCAGGACC ACGAGTGTGT GTTTCAGGCT CAGAGCCGCT GGAAGGGGGC TGGAAAATTC
    ATCCTCAAAC TCAAAGAGCA GTTGGCTCGT GAGGACAAGC GTAAAGGTGT GTCGTTCGCC
    AGTGAGCTGA GGAAGCTGAC GGGTCGCAGT CGCAGTGTGA CGGTCAACAC AAACAGCAAC
    GACACTCACA GGTCTCTGTC TCTGGGGCTG AAGGCCTGGA TGGCTGAGGG CCGCTTGGGC
    TCTCCGAGAC GCTCCCAGTG TGGTGACGGT GTCTCGGCCT TGTCCGGGTG GTCTCTCCCG
    CTGCTGTGGA ACTGGAAGCT GCATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335822.1
    CDS432..7301
    Translation

    Target ORF information:

    RefSeq Version XM_021480147.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480147.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GAAAGGACCT
    GGGACTGAAC GAGTCCTGTT TTCAATCGGC GACCTCTCAG ACTCTGAAGG GGACCCACTT
    GAGACAGTTA AGGATGTTGT AGACCTCCAG ACCACCGAAG ATGTTCGCGG ACCCTTTAGC
    CATGGGACTG AGCTCATTCC CTGGTACGTT CTGTCGCTTC AGCCGGATAT CCATCAGTTC
    CTGCTCCAAG GAGCAACCGT CATCCACTAC GACCCCGAGA GCCACCTCAC CGCCCGCTGT
    CTTCTCCGTC TACAGCCCGA TAACTGCTTC CTCACCTGGT GCAAACCCCA CAGTAGCTGC
    AGCCTATATG GAAGAGCTAG AACCTTCATG GGACATCCAG CATCCCCAGA CCACTTACAC
    ATCGGCCAAC CTGTTCACTG CGGACTCTCT GACGGGCTCC TGGATCTTAA TGTGGTGAAG
    GCTGTGTTTA TGGGTCACCC TGGAGTGGAC GTCAACTATG TCTGCCTGCA GCACAAACTG
    TGTAACATGA ATCCTGGTGA AAATGGTGTC ACGCTGCTAT ATGGACTTCA CACCACAGAC
    AACAGGCTCC TGCACTTTGT GGCCCCGAAA CACACGGCTC GCATGCTGCA CGAGGGCCTC
    CAGGAGCTGC TGAACTCTAT TAGGAAGATC AGGAAATTCC CTGACCAGAG ACTGCAATGG
    CTGAGAAAGC AATATGTTAG TTTGTACCAG GAGGATGGTA GATTTGAAGG ACCTACACTG
    GCTCATGCTA TCGAGCTGTT CGGAGGGAGA CGCTGGAATA TGGGAACTAG CGGCCCTGGA
    TCCGCCAGCA GAGGTGCAGA GAAGAACTCG GCTCAGAAGA ACAGTCCACT GGGCATCAAC
    TCCAATGTCA AAAAAAAGAA AAAGGCCTTA GTCAGGGGGG ACAGTGGCGA TGGAACTGAT
    GATGAGATGA CCGCACGCAA GACCAGGAGT TGCAAAGAGA CTTTGGGGAG GAGAGAGTCA
    GATGCTCTGG AAAATGTTGA GCAGGAAGAG GCTGAGGACT GCAGTACCTT TAGCCAACCT
    GGACCATTTT CCTTATCCAA CAACAAAGGC CAGTCTCCGG GATCCTCCTC GTCCTCCTCC
    ACCTCTTCTT CTGTTGCCTC CTTTTGCACA AACTCCTCCA CCCCTACACG TCCACATTCC
    TCCCCAATCC TCCCCACAAA CCTCAAGAGT CAGCCAGGGG CCTGGAGCAG TCGAAGCTGG
    CACGGTCGAG GGAAAGGCTG CTTCAGAGGT TTCCAGGACC TCATGATCTC CGACAGCATC
    ATGAGCTTTG TCGAATTTGT GGAGCTCTTC AAATCTTTTA GCATTCGCAG TCGTAAGGAT
    CTGAAGGAAC TATTCGACAC CTACGCCGTG CCCTGCAGTC GCTCTGGCCC TGAGTCTGTC
    CCCCTCTACA CCACACTTAG GATTGACGAC AAGCTAACCG GACTGCAGCC AGATCTGGAT
    CTCCTCACTC GCAACGGCTC TGACCTGGGC CTTTTCATTC GCACCCGCCA GCAAATGTCT
    GAAAACCAGA AGCAGATTTC AGACGCCATC GCAGCTGCCA GTATTGTGAC CAATGGCACT
    GGAGTGGAGA ATTCATCGCT GGGTGTGCTG GGACTGGCCA TCTCGCAGCT CAATGATTTC
    TTGGTCAACT GTCAGAGAGA ACATCTGAGC TATGATGAGA TCCTCAGCAT CATACAGAAA
    TTTGAGCCTT CTTCAAACAT GCGTCAGATG GGGTGGATGT CGTTTGAAGG ATTCGCCAGG
    TTTCTAATGG ACAAGGACAA CTTTGCCTCC AAAAATGAGG AGTCTCAAGT GAACCTGGAT
    GAGCTCCAGC ATCCGCTGTC TTACTATTAC ATCGAGTCCT CTCATAACAC ATACCTGACG
    GGGCATCAGC TGAAGGGAGA GTCTTCAGTC GAGCTCTACA GCCAGGTGTT GCTGCAGGGT
    TGTCGCAGCG TTGAGTTGGA CTGCTGGGAT GGGGATGACG GCATGCCTGT TATTTACCAT
    GGACATACTC TTACCACTAA GATTCCTTTC AAGGATGTTG TAGAGGCCAT CAACCGTGCA
    GCATTTGTGA ACTCAGAGAT GCCTGTGATT CTGTCCATTG AAAACCACTG CTCCTTACCA
    CAGCAGCGCA AAATGGCTGA GATTTTTAAG ATGGTGTTTG GTGAGAAGCT GGTGACCAAG
    TTTCTGTTTG AAAGTGATTT CGCGGACGAG CCTTTGCTTC CGTCTCCCCT TCAGCTCCGG
    GGGAAAATCC TCCTGAAGAA TAAAAAGTTA AAAGCTCACC AGGCTCCTGT TGACATTCTC
    AAACAAAAGG CGCATCAGCT TGCCCACATG CAGGCTCAGG CCAACAACGG CACAGTCAGC
    ACAACGCCAC TGGGAAACAA TGATGAAGAG GAGGAGGAGG AAGACGAGTA CGATTACGAT
    TACGAGTCGC TGTCTGACGC TGATGTCCTC AATGCTTCCA CAGCCTCTTA TGGCCAGGAA
    GACAATATTC TGGATGACAA ACCTGAAGGC AAGTCATCTA CGGAGAAACT TCAGTACGAG
    TCCAACGATG AGATGCCAAA GCGGTTTAAA AAAGCTGGAA GCAAAGGAAA GATGTTTGAC
    ATGGAGCTGG GAGAGGAGTT TTACCTGCCC CAGAACGAGA AGGAGAGTAG ACAGATTGCT
    CAAGAGCTGT CAGATCTGGT CATCTACTGC CAGGCTATCA AATTTCCAGG CCTCTCCACC
    CTCTCTCCAT CAGGCTCTGG AAGGGGGAAA GACAGAAAGA GCCGGAAATC CATCTTTGGC
    AGCGCTCCTG CTCGCGGGAG CCCCGGGGAG CCTGTGACGC TGGTCCGAGC TCCAGGGAAA
    GCCAGTCTTG AGGGCATTCG CATGAACTCG GAGGATCAAT TGTGTCTGAG CCCGAGCACC
    TCTCTGAGCT CCATCATCCG CACGCCCAAA TGCTACCACA TCTCCTCTGT GAACGAGAAT
    GCAGCGAAGC GCTTGTGTCG CCGCTACTCG CAGAAGCTGA TCCAGCACAC CAGCTGCCAG
    CTGCTGCGCA CGTACCCAGC GGCCACACGC ATTGACTCTG CCAACCCCAA TCCGCTCATC
    TTCTGGCTGC ACGGAGTCCA GCTGGTGGCC CTCAACTACC AGACTGATGA TCTGCCGATG
    CAGCTGAACG CGGCTCTATT CGAGGCTAAT GGTCACTGTG GTTTTGTGCT GAAGCCTCCA
    GTCCTGTGGG ACAGAAGCTG TCCTCTCTAT CAGCACTTTT ACCCTCTGGA CCGAGACCTG
    GAGAACATGA CCCCAACGCT ATACACACTC ACTATTGTGT CGGGGCAGAA CGTGTGTCCA
    GGAAACTCAA ACGGCAGTCC GTGTGTCGAA GTGGAGGTGT TGGGAATGCC GGCGGACAGC
    TGTCATTTCC GAACTAAACC CATACACCGC AACACACTCA ATCCCATGTG GAACGAACAC
    TTCCAGTTCC ACGTGCACTT TGAAGACCTG GCGTTCCTGC GCATCGCTGT GGTGGAGAAC
    AACAGCTCTC AGGTCACCGC TCAGAGGATA CTTCCCCTCA AAACACTCAG AGCAGGCTAC
    CGGCACCTGC AGCTGAGGAA CCTGCACAAC GAGCCTCTGG AGGTGTCCAG TCTGTTCATG
    TTCAGCCGCA GGACGGAGGA GAGCCCGACC GGAGGACCAC CGTCTGCGTC TGTGCTGTTC
    AGCACCGAGG AGAGACGATC TGTGCAGCAG CACAAGGTGA CCGTGCATGG AGTCCCCGGA
    CCTGAACCCT TCACTGTGAT CTGTGTCGAT GAATTCACCA CAGCCAAACA GCTCCTGGAC
    TCTCTGTTTC CGACTGCCTC TTTTAAGTAT ATGCTGATGG AGGAGAGAGT GTCTCTGTCG
    AAGGAGAAGA AGGCTCCTCA GCAGCGTCCT CTAGCCAATG ATGAGAGACT GCTGAAGCTC
    ATTCACAGCT GGCAGCCTGA GGACGGATAT GTGGGCAGAA TATACCTCAA AACCAGAGAA
    CAGCAGAACT GCAGTGAGAA GACGAGCGTC CCGCTGGAGT CACTGGAGGA GCTCGGCAGC
    TTAGAGGACG ACACCTTCTT CGTGCAGGTC CATGATGTTT CTCCAGAACA GCCTCACACC
    GTCATCAAAG CCCCGCGATA CAGCACAGCA CAGGACATCA TCCAGCAGAC TCTGAGCAAA
    GCCAAGTATT CTCTGAGTAT CCTGAGCAAC CCCAACCCAT GTGACTACGT CCTGATGGAG
    GAGCTTTGTA AAGACGCGGG TGGAAAGAAG TCGTCCTCTG CCAAACCGTG CCAGCGTGTG
    CTGCAGGACC ACGAGTGTGT GTTTCAGGCT CAGAGCCGCT GGAAGGGGGC TGGAAAATTC
    ATCCTCAAAC TCAAAGAGCA GTTGGCTCGT GAGGACAAGC GTAAAGGTGT GTCGTTCGCC
    AGTGAGCTGA GGAAGCTGAC GGGTCGCAGT CGCAGTGTGA CGGTCAACAC AAACAGCAAC
    GACACTCACA GGTCTCTGTC TCTGGGGCTG AAGGCCTGGA TGGCTGAGGG CCGCTTGGGC
    TCTCCGAGAC GCTCCCAGTG TGGTGACGGT GTCTCGGCCT TGTCCGGGTG GTCTCTCCCG
    CTGCTGTGGA ACTGGAAGCT GCATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148221
    Clone ID Related Accession (Same CDS sequence) XM_021480148.1
    Accession Version XM_021480148.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GAAAGGACCT
    GGGACTGAAC GAGTCCTGTT TTCAATCGGC GACCTCTCAG ACTCTGAAGG GGACCCACTT
    GAGACAGTTA AGGATGTTGT AGACCTCCAG ACCACCGAAG ATGTTCGCGG ACCCTTTAGC
    CATGGGACTG AGCTCATTCC CTGGTACGTT CTGTCGCTTC AGCCGGATAT CCATCAGTTC
    CTGCTCCAAG GAGCAACCGT CATCCACTAC GACCCCGAGA GCCACCTCAC CGCCCGCTGT
    CTTCTCCGTC TACAGCCCGA TAACTGCTTC CTCACCTGGT GCAAACCCCA CAGTAGCTGC
    AGCCTATATG GAAGAGCTAG AACCTTCATG GGACATCCAG CATCCCCAGA CCACTTACAC
    ATCGGCCAAC CTGTTCACTG CGGACTCTCT GACGGGCTCC TGGATCTTAA TGTGGTGAAG
    GCTGTGTTTA TGGGTCACCC TGGAGTGGAC GTCAACTATG TCTGCCTGCA GCACAAACTG
    TGTAACATGA ATCCTGGTGA AAATGGTGTC ACGCTGCTAT ATGGACTTCA CACCACAGAC
    AACAGGCTCC TGCACTTTGT GGCCCCGAAA CACACGGCTC GCATGCTGCA CGAGGGCCTC
    CAGGAGCTGC TGAACTCTAT TAGGAAGATC AGGAAATTCC CTGACCAGAG ACTGCAATGG
    CTGAGAAAGC AATATGTTAG TTTGTACCAG GAGGATGGTA GATTTGAAGG ACCTACACTG
    GCTCATGCTA TCGAGCTGTT CGGAGGGAGA CGCTGGAATA TGGGAACTAG CGGCCCTGGA
    TCCGCCAGCA GAGGTGCAGA GAAGAACTCG GCTCAGAAGA ACAGTCCACT GGGCATCAAC
    TCCAATGTCA AAAAAAAGAA AAAGGCCTTA GTCAGGGGGG ACAGTGGCGA TGGAACTGAT
    GATGAGATGA CCGCACGCAA GACCAGGAGT TGCAAAGAGA CTTTGGGGAG GAGAGAGTCA
    GATGCTCTGG AAAATGTTGA GCAGGAAGAG GCTGAGGACT GCAGTACCTT TAGCCAACCT
    GGACCATTTT CCTTATCCAA CAACAAAGGC CAGTCTCCGG GATCCTCCTC GTCCTCCTCC
    ACCTCTTCTT CTGTTGCCTC CTTTTGCACA AACTCCTCCA CCCCTACACG TCCACATTCC
    TCCCCAATCC TCCCCACAAA CCTCAAGAGT CAGCCAGGGG CCTGGAGCAG TCGAAGCTGG
    CACGGTCGAG GGAAAGGCTG CTTCAGAGGT TTCCAGGACC TCATGATCTC CGACAGCATC
    ATGAGCTTTG TCGAATTTGT GGAGCTCTTC AAATCTTTTA GCATTCGCAG TCGTAAGGAT
    CTGAAGGAAC TATTCGACAC CTACGCCGTG CCCTGCAGTC GCTCTGGCCC TGAGTCTGTC
    CCCCTCTACA CCACACTTAG GATTGACGAC AAGCTAACCG GACTGCAGCC AGATCTGGAT
    CTCCTCACTC GCAACGGCTC TGACCTGGGC CTTTTCATTC GCACCCGCCA GCAAATGTCT
    GAAAACCAGA AGCAGATTTC AGACGCCATC GCAGCTGCCA GTATTGTGAC CAATGGCACT
    GGAGTGGAGA ATTCATCGCT GGGTGTGCTG GGACTGGCCA TCTCGCAGCT CAATGATTTC
    TTGGTCAACT GTCAGAGAGA ACATCTGAGC TATGATGAGA TCCTCAGCAT CATACAGAAA
    TTTGAGCCTT CTTCAAACAT GCGTCAGATG GGGTGGATGT CGTTTGAAGG ATTCGCCAGG
    TTTCTAATGG ACAAGGACAA CTTTGCCTCC AAAAATGAGG AGTCTCAAGT GAACCTGGAT
    GAGCTCCAGC ATCCGCTGTC TTACTATTAC ATCGAGTCCT CTCATAACAC ATACCTGACG
    GGGCATCAGC TGAAGGGAGA GTCTTCAGTC GAGCTCTACA GCCAGGTGTT GCTGCAGGGT
    TGTCGCAGCG TTGAGTTGGA CTGCTGGGAT GGGGATGACG GCATGCCTGT TATTTACCAT
    GGACATACTC TTACCACTAA GATTCCTTTC AAGGATGTTG TAGAGGCCAT CAACCGTGCA
    GCATTTGTGA ACTCAGAGAT GCCTGTGATT CTGTCCATTG AAAACCACTG CTCCTTACCA
    CAGCAGCGCA AAATGGCTGA GATTTTTAAG ATGGTGTTTG GTGAGAAGCT GGTGACCAAG
    TTTCTGTTTG AAAGTGATTT CGCGGACGAG CCTTTGCTTC CGTCTCCCCT TCAGCTCCGG
    GGGAAAATCC TCCTGAAGAA TAAAAAGTTA AAAGCTCACC AGGCTCCTGT TGACATTCTC
    AAACAAAAGG CGCATCAGCT TGCCCACATG CAGGCTCAGG CCAACAACGG CACAGTCAGC
    ACAACGCCAC TGGGAAACAA TGATGAAGAG GAGGAGGAGG AAGACGAGTA CGATTACGAT
    TACGAGTCGC TGTCTGACGC TGATGTCCTC AATGCTTCCA CAGCCTCTTA TGGCCAGGAA
    GACAATATTC TGGATGACAA ACCTGAAGGC AAGTCATCTA CGGAGAAACT TCAGTACGAG
    TCCAACGATG AGATGCCAAA GCGGTTTAAA AAAGCTGGAA GCAAAGGAAA GATGTTTGAC
    ATGGAGCTGG GAGAGGAGTT TTACCTGCCC CAGAACGAGA AGGAGAGTAG ACAGATTGCT
    CAAGAGCTGT CAGATCTGGT CATCTACTGC CAGGCTATCA AATTTCCAGG CCTCTCCACC
    CTCTCTCCAT CAGGCTCTGG AAGGGGGAAA GACAGAAAGA GCCGGAAATC CATCTTTGGC
    AGCGCTCCTG CTCGCGGGAG CCCCGGGGAG CCTGTGACGC TGGTCCGAGC TCCAGGGAAA
    GCCAGTCTTG AGGGCATTCG CATGAACTCG GAGGATCAAT TGTGTCTGAG CCCGAGCACC
    TCTCTGAGCT CCATCATCCG CACGCCCAAA TGCTACCACA TCTCCTCTGT GAACGAGAAT
    GCAGCGAAGC GCTTGTGTCG CCGCTACTCG CAGAAGCTGA TCCAGCACAC CAGCTGCCAG
    CTGCTGCGCA CGTACCCAGC GGCCACACGC ATTGACTCTG CCAACCCCAA TCCGCTCATC
    TTCTGGCTGC ACGGAGTCCA GCTGGTGGCC CTCAACTACC AGACTGATGA TCTGCCGATG
    CAGCTGAACG CGGCTCTATT CGAGGCTAAT GGTCACTGTG GTTTTGTGCT GAAGCCTCCA
    GTCCTGTGGG ACAGAAGCTG TCCTCTCTAT CAGCACTTTT ACCCTCTGGA CCGAGACCTG
    GAGAACATGA CCCCAACGCT ATACACACTC ACTATTGTGT CGGGGCAGAA CGTGTGTCCA
    GGAAACTCAA ACGGCAGTCC GTGTGTCGAA GTGGAGGTGT TGGGAATGCC GGCGGACAGC
    TGTCATTTCC GAACTAAACC CATACACCGC AACACACTCA ATCCCATGTG GAACGAACAC
    TTCCAGTTCC ACGTGCACTT TGAAGACCTG GCGTTCCTGC GCATCGCTGT GGTGGAGAAC
    AACAGCTCTC AGGTCACCGC TCAGAGGATA CTTCCCCTCA AAACACTCAG AGCAGGCTAC
    CGGCACCTGC AGCTGAGGAA CCTGCACAAC GAGCCTCTGG AGGTGTCCAG TCTGTTCATG
    TTCAGCCGCA GGACGGAGGA GAGCCCGACC GGAGGACCAC CGTCTGCGTC TGTGCTGTTC
    AGCACCGAGG AGAGACGATC TGTGCAGCAG CACAAGGTGA CCGTGCATGG AGTCCCCGGA
    CCTGAACCCT TCACTGTGAT CTGTGTCGAT GAATTCACCA CAGCCAAACA GCTCCTGGAC
    TCTCTGTTTC CGACTGCCTC TTTTAAGTAT ATGCTGATGG AGGAGAGAGT GTCTCTGTCG
    AAGGAGAAGA AGGCTCCTCA GCAGCGTCCT CTAGCCAATG ATGAGAGACT GCTGAAGCTC
    ATTCACAGCT GGCAGCCTGA GGACGGATAT GTGGGCAGAA TATACCTCAA AACCAGAGAA
    CAGAACTGCA GTGAGAAGAC GAGCGTCCCG CTGGAGTCAC TGGAGGAGCT CGGCAGCTTA
    GAGGACGACA CCTTCTTCGT GCAGGTCCAT GATGTTTCTC CAGAACAGCC TCACACCGTC
    ATCAAAGCCC CGCGATACAG CACAGCACAG GACATCATCC AGCAGACTCT GAGCAAAGCC
    AAGTATTCTC TGAGTATCCT GAGCAACCCC AACCCATGTG ACTACGTCCT GATGGAGGAG
    CTTTGTAAAG ACGCGGGTGG AAAGAAGTCG TCCTCTGCCA AACCGTGCCA GCGTGTGCTG
    CAGGACCACG AGTGTGTGTT TCAGGCTCAG AGCCGCTGGA AGGGGGCTGG AAAATTCATC
    CTCAAACTCA AAGAGCAGTT GGCTCGTGAG GACAAGCGTA AAGGTGTGTC GTTCGCCAGT
    GAGCTGAGGA AGCTGACGGG TCGCAGTCGC AGTGTGACGG TCAACACAAA CAGCAACGAC
    ACTCACAGGT CTCTGTCTCT GGGGCTGAAG GCCTGGATGG CTGAGGGCCG CTTGGGCTCT
    CCGAGACGCT CCCAGTGTGG TGACGGTGTC TCGGCCTTGT CCGGGTGGTC TCTCCCGCTG
    CTGTGGAACT GGAAGCTGCA TAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335823.1
    CDS432..7298
    Translation

    Target ORF information:

    RefSeq Version XM_021480148.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480148.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GAAAGGACCT
    GGGACTGAAC GAGTCCTGTT TTCAATCGGC GACCTCTCAG ACTCTGAAGG GGACCCACTT
    GAGACAGTTA AGGATGTTGT AGACCTCCAG ACCACCGAAG ATGTTCGCGG ACCCTTTAGC
    CATGGGACTG AGCTCATTCC CTGGTACGTT CTGTCGCTTC AGCCGGATAT CCATCAGTTC
    CTGCTCCAAG GAGCAACCGT CATCCACTAC GACCCCGAGA GCCACCTCAC CGCCCGCTGT
    CTTCTCCGTC TACAGCCCGA TAACTGCTTC CTCACCTGGT GCAAACCCCA CAGTAGCTGC
    AGCCTATATG GAAGAGCTAG AACCTTCATG GGACATCCAG CATCCCCAGA CCACTTACAC
    ATCGGCCAAC CTGTTCACTG CGGACTCTCT GACGGGCTCC TGGATCTTAA TGTGGTGAAG
    GCTGTGTTTA TGGGTCACCC TGGAGTGGAC GTCAACTATG TCTGCCTGCA GCACAAACTG
    TGTAACATGA ATCCTGGTGA AAATGGTGTC ACGCTGCTAT ATGGACTTCA CACCACAGAC
    AACAGGCTCC TGCACTTTGT GGCCCCGAAA CACACGGCTC GCATGCTGCA CGAGGGCCTC
    CAGGAGCTGC TGAACTCTAT TAGGAAGATC AGGAAATTCC CTGACCAGAG ACTGCAATGG
    CTGAGAAAGC AATATGTTAG TTTGTACCAG GAGGATGGTA GATTTGAAGG ACCTACACTG
    GCTCATGCTA TCGAGCTGTT CGGAGGGAGA CGCTGGAATA TGGGAACTAG CGGCCCTGGA
    TCCGCCAGCA GAGGTGCAGA GAAGAACTCG GCTCAGAAGA ACAGTCCACT GGGCATCAAC
    TCCAATGTCA AAAAAAAGAA AAAGGCCTTA GTCAGGGGGG ACAGTGGCGA TGGAACTGAT
    GATGAGATGA CCGCACGCAA GACCAGGAGT TGCAAAGAGA CTTTGGGGAG GAGAGAGTCA
    GATGCTCTGG AAAATGTTGA GCAGGAAGAG GCTGAGGACT GCAGTACCTT TAGCCAACCT
    GGACCATTTT CCTTATCCAA CAACAAAGGC CAGTCTCCGG GATCCTCCTC GTCCTCCTCC
    ACCTCTTCTT CTGTTGCCTC CTTTTGCACA AACTCCTCCA CCCCTACACG TCCACATTCC
    TCCCCAATCC TCCCCACAAA CCTCAAGAGT CAGCCAGGGG CCTGGAGCAG TCGAAGCTGG
    CACGGTCGAG GGAAAGGCTG CTTCAGAGGT TTCCAGGACC TCATGATCTC CGACAGCATC
    ATGAGCTTTG TCGAATTTGT GGAGCTCTTC AAATCTTTTA GCATTCGCAG TCGTAAGGAT
    CTGAAGGAAC TATTCGACAC CTACGCCGTG CCCTGCAGTC GCTCTGGCCC TGAGTCTGTC
    CCCCTCTACA CCACACTTAG GATTGACGAC AAGCTAACCG GACTGCAGCC AGATCTGGAT
    CTCCTCACTC GCAACGGCTC TGACCTGGGC CTTTTCATTC GCACCCGCCA GCAAATGTCT
    GAAAACCAGA AGCAGATTTC AGACGCCATC GCAGCTGCCA GTATTGTGAC CAATGGCACT
    GGAGTGGAGA ATTCATCGCT GGGTGTGCTG GGACTGGCCA TCTCGCAGCT CAATGATTTC
    TTGGTCAACT GTCAGAGAGA ACATCTGAGC TATGATGAGA TCCTCAGCAT CATACAGAAA
    TTTGAGCCTT CTTCAAACAT GCGTCAGATG GGGTGGATGT CGTTTGAAGG ATTCGCCAGG
    TTTCTAATGG ACAAGGACAA CTTTGCCTCC AAAAATGAGG AGTCTCAAGT GAACCTGGAT
    GAGCTCCAGC ATCCGCTGTC TTACTATTAC ATCGAGTCCT CTCATAACAC ATACCTGACG
    GGGCATCAGC TGAAGGGAGA GTCTTCAGTC GAGCTCTACA GCCAGGTGTT GCTGCAGGGT
    TGTCGCAGCG TTGAGTTGGA CTGCTGGGAT GGGGATGACG GCATGCCTGT TATTTACCAT
    GGACATACTC TTACCACTAA GATTCCTTTC AAGGATGTTG TAGAGGCCAT CAACCGTGCA
    GCATTTGTGA ACTCAGAGAT GCCTGTGATT CTGTCCATTG AAAACCACTG CTCCTTACCA
    CAGCAGCGCA AAATGGCTGA GATTTTTAAG ATGGTGTTTG GTGAGAAGCT GGTGACCAAG
    TTTCTGTTTG AAAGTGATTT CGCGGACGAG CCTTTGCTTC CGTCTCCCCT TCAGCTCCGG
    GGGAAAATCC TCCTGAAGAA TAAAAAGTTA AAAGCTCACC AGGCTCCTGT TGACATTCTC
    AAACAAAAGG CGCATCAGCT TGCCCACATG CAGGCTCAGG CCAACAACGG CACAGTCAGC
    ACAACGCCAC TGGGAAACAA TGATGAAGAG GAGGAGGAGG AAGACGAGTA CGATTACGAT
    TACGAGTCGC TGTCTGACGC TGATGTCCTC AATGCTTCCA CAGCCTCTTA TGGCCAGGAA
    GACAATATTC TGGATGACAA ACCTGAAGGC AAGTCATCTA CGGAGAAACT TCAGTACGAG
    TCCAACGATG AGATGCCAAA GCGGTTTAAA AAAGCTGGAA GCAAAGGAAA GATGTTTGAC
    ATGGAGCTGG GAGAGGAGTT TTACCTGCCC CAGAACGAGA AGGAGAGTAG ACAGATTGCT
    CAAGAGCTGT CAGATCTGGT CATCTACTGC CAGGCTATCA AATTTCCAGG CCTCTCCACC
    CTCTCTCCAT CAGGCTCTGG AAGGGGGAAA GACAGAAAGA GCCGGAAATC CATCTTTGGC
    AGCGCTCCTG CTCGCGGGAG CCCCGGGGAG CCTGTGACGC TGGTCCGAGC TCCAGGGAAA
    GCCAGTCTTG AGGGCATTCG CATGAACTCG GAGGATCAAT TGTGTCTGAG CCCGAGCACC
    TCTCTGAGCT CCATCATCCG CACGCCCAAA TGCTACCACA TCTCCTCTGT GAACGAGAAT
    GCAGCGAAGC GCTTGTGTCG CCGCTACTCG CAGAAGCTGA TCCAGCACAC CAGCTGCCAG
    CTGCTGCGCA CGTACCCAGC GGCCACACGC ATTGACTCTG CCAACCCCAA TCCGCTCATC
    TTCTGGCTGC ACGGAGTCCA GCTGGTGGCC CTCAACTACC AGACTGATGA TCTGCCGATG
    CAGCTGAACG CGGCTCTATT CGAGGCTAAT GGTCACTGTG GTTTTGTGCT GAAGCCTCCA
    GTCCTGTGGG ACAGAAGCTG TCCTCTCTAT CAGCACTTTT ACCCTCTGGA CCGAGACCTG
    GAGAACATGA CCCCAACGCT ATACACACTC ACTATTGTGT CGGGGCAGAA CGTGTGTCCA
    GGAAACTCAA ACGGCAGTCC GTGTGTCGAA GTGGAGGTGT TGGGAATGCC GGCGGACAGC
    TGTCATTTCC GAACTAAACC CATACACCGC AACACACTCA ATCCCATGTG GAACGAACAC
    TTCCAGTTCC ACGTGCACTT TGAAGACCTG GCGTTCCTGC GCATCGCTGT GGTGGAGAAC
    AACAGCTCTC AGGTCACCGC TCAGAGGATA CTTCCCCTCA AAACACTCAG AGCAGGCTAC
    CGGCACCTGC AGCTGAGGAA CCTGCACAAC GAGCCTCTGG AGGTGTCCAG TCTGTTCATG
    TTCAGCCGCA GGACGGAGGA GAGCCCGACC GGAGGACCAC CGTCTGCGTC TGTGCTGTTC
    AGCACCGAGG AGAGACGATC TGTGCAGCAG CACAAGGTGA CCGTGCATGG AGTCCCCGGA
    CCTGAACCCT TCACTGTGAT CTGTGTCGAT GAATTCACCA CAGCCAAACA GCTCCTGGAC
    TCTCTGTTTC CGACTGCCTC TTTTAAGTAT ATGCTGATGG AGGAGAGAGT GTCTCTGTCG
    AAGGAGAAGA AGGCTCCTCA GCAGCGTCCT CTAGCCAATG ATGAGAGACT GCTGAAGCTC
    ATTCACAGCT GGCAGCCTGA GGACGGATAT GTGGGCAGAA TATACCTCAA AACCAGAGAA
    CAGAACTGCA GTGAGAAGAC GAGCGTCCCG CTGGAGTCAC TGGAGGAGCT CGGCAGCTTA
    GAGGACGACA CCTTCTTCGT GCAGGTCCAT GATGTTTCTC CAGAACAGCC TCACACCGTC
    ATCAAAGCCC CGCGATACAG CACAGCACAG GACATCATCC AGCAGACTCT GAGCAAAGCC
    AAGTATTCTC TGAGTATCCT GAGCAACCCC AACCCATGTG ACTACGTCCT GATGGAGGAG
    CTTTGTAAAG ACGCGGGTGG AAAGAAGTCG TCCTCTGCCA AACCGTGCCA GCGTGTGCTG
    CAGGACCACG AGTGTGTGTT TCAGGCTCAG AGCCGCTGGA AGGGGGCTGG AAAATTCATC
    CTCAAACTCA AAGAGCAGTT GGCTCGTGAG GACAAGCGTA AAGGTGTGTC GTTCGCCAGT
    GAGCTGAGGA AGCTGACGGG TCGCAGTCGC AGTGTGACGG TCAACACAAA CAGCAACGAC
    ACTCACAGGT CTCTGTCTCT GGGGCTGAAG GCCTGGATGG CTGAGGGCCG CTTGGGCTCT
    CCGAGACGCT CCCAGTGTGG TGACGGTGTC TCGGCCTTGT CCGGGTGGTC TCTCCCGCTG
    CTGTGGAACT GGAAGCTGCA TAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148222
    Clone ID Related Accession (Same CDS sequence) XM_021480149.1
    Accession Version XM_021480149.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GTTTTCAATC
    GGCGACCTCT CAGACTCTGA AGGGGACCCA CTTGAGACAG TTAAGGATGT TGTAGACCTC
    CAGACCACCG AAGATGTTCG CGGACCCTTT AGCCATGGGA CTGAGCTCAT TCCCTGGTAC
    GTTCTGTCGC TTCAGCCGGA TATCCATCAG TTCCTGCTCC AAGGAGCAAC CGTCATCCAC
    TACGACCCCG AGAGCCACCT CACCGCCCGC TGTCTTCTCC GTCTACAGCC CGATAACTGC
    TTCCTCACCT GGTGCAAACC CCACAGTAGC TGCAGCCTAT ATGGAAGAGC TAGAACCTTC
    ATGGGACATC CAGCATCCCC AGACCACTTA CACATCGGCC AACCTGTTCA CTGCGGACTC
    TCTGACGGGC TCCTGGATCT TAATGTGGTG AAGGCTGTGT TTATGGGTCA CCCTGGAGTG
    GACGTCAACT ATGTCTGCCT GCAGCACAAA CTGTGTAACA TGAATCCTGG TGAAAATGGT
    GTCACGCTGC TATATGGACT TCACACCACA GACAACAGGC TCCTGCACTT TGTGGCCCCG
    AAACACACGG CTCGCATGCT GCACGAGGGC CTCCAGGAGC TGCTGAACTC TATTAGGAAG
    ATCAGGAAAT TCCCTGACCA GAGACTGCAA TGGCTGAGAA AGCAATATGT TAGTTTGTAC
    CAGGAGGATG GTAGATTTGA AGGACCTACA CTGGCTCATG CTATCGAGCT GTTCGGAGGG
    AGACGCTGGA ATATGGGAAC TAGCGGCCCT GGATCCGCCA GCAGAGGTGC AGAGAAGAAC
    TCGGCTCAGA AGAACAGTCC ACTGGGCATC AACTCCAATG TCAAAAAAAA GAAAAAGGCC
    TTAGTCAGGG GGGACAGTGG CGATGGAACT GATGATGAGA TGACCGCACG CAAGACCAGG
    AGTTGCAAAG AGACTTTGGG GAGGAGAGAG TCAGATGCTC TGGAAAATGT TGAGCAGGAA
    GAGGCTGAGG ACTGCAGTAC CTTTAGCCAA CCTGGACCAT TTTCCTTATC CAACAACAAA
    GGCCAGTCTC CGGGATCCTC CTCGTCCTCC TCCACCTCTT CTTCTGTTGC CTCCTTTTGC
    ACAAACTCCT CCACCCCTAC ACGTCCACAT TCCTCCCCAA TCCTCCCCAC AAACCTCAAG
    AGTCAGCCAG GGGCCTGGAG CAGTCGAAGC TGGCACGGTC GAGGGAAAGG CTGCTTCAGA
    GGTTTCCAGG ACCTCATGAT CTCCGACAGC ATCATGAGCT TTGTCGAATT TGTGGAGCTC
    TTCAAATCTT TTAGCATTCG CAGTCGTAAG GATCTGAAGG AACTATTCGA CACCTACGCC
    GTGCCCTGCA GTCGCTCTGG CCCTGAGTCT GTCCCCCTCT ACACCACACT TAGGATTGAC
    GACAAGCTAA CCGGACTGCA GCCAGATCTG GATCTCCTCA CTCGCAACGG CTCTGACCTG
    GGCCTTTTCA TTCGCACCCG CCAGCAAATG TCTGAAAACC AGAAGCAGAT TTCAGACGCC
    ATCGCAGCTG CCAGTATTGT GACCAATGGC ACTGGAGTGG AGAATTCATC GCTGGGTGTG
    CTGGGACTGG CCATCTCGCA GCTCAATGAT TTCTTGGTCA ACTGTCAGAG AGAACATCTG
    AGCTATGATG AGATCCTCAG CATCATACAG AAATTTGAGC CTTCTTCAAA CATGCGTCAG
    ATGGGGTGGA TGTCGTTTGA AGGATTCGCC AGGTTTCTAA TGGACAAGGA CAACTTTGCC
    TCCAAAAATG AGGAGTCTCA AGTGAACCTG GATGAGCTCC AGCATCCGCT GTCTTACTAT
    TACATCGAGT CCTCTCATAA CACATACCTG ACGGGGCATC AGCTGAAGGG AGAGTCTTCA
    GTCGAGCTCT ACAGCCAGGT GTTGCTGCAG GGTTGTCGCA GCGTTGAGTT GGACTGCTGG
    GATGGGGATG ACGGCATGCC TGTTATTTAC CATGGACATA CTCTTACCAC TAAGATTCCT
    TTCAAGGATG TTGTAGAGGC CATCAACCGT GCAGCATTTG TGAACTCAGA GATGCCTGTG
    ATTCTGTCCA TTGAAAACCA CTGCTCCTTA CCACAGCAGC GCAAAATGGC TGAGATTTTT
    AAGATGGTGT TTGGTGAGAA GCTGGTGACC AAGTTTCTGT TTGAAAGTGA TTTCGCGGAC
    GAGCCTTTGC TTCCGTCTCC CCTTCAGCTC CGGGGGAAAA TCCTCCTGAA GAATAAAAAG
    TTAAAAGCTC ACCAGGCTCC TGTTGACATT CTCAAACAAA AGGCGCATCA GCTTGCCCAC
    ATGCAGGCTC AGGCCAACAA CGGCACAGTC AGCACAACGC CACTGGGAAA CAATGATGAA
    GAGGAGGAGG AGGAAGACGA GTACGATTAC GATTACGAGT CGCTGTCTGA CGCTGATGTC
    CTCAATGCTT CCACAGCCTC TTATGGCCAG GAAGACAATA TTCTGGATGA CAAACCTGAA
    GGCAAGTCAT CTACGGAGAA ACTTCAGTAC GAGTCCAACG ATGAGATGCC AAAGCGGTTT
    AAAAAAGCTG GAAGCAAAGG AAAGATGTTT GACATGGAGC TGGGAGAGGA GTTTTACCTG
    CCCCAGAACG AGAAGGAGAG TAGACAGATT GCTCAAGAGC TGTCAGATCT GGTCATCTAC
    TGCCAGGCTA TCAAATTTCC AGGCCTCTCC ACCCTCTCTC CATCAGGCTC TGGAAGGGGG
    AAAGACAGAA AGAGCCGGAA ATCCATCTTT GGCAGCGCTC CTGCTCGCGG GAGCCCCGGG
    GAGCCTGTGA CGCTGGTCCG AGCTCCAGGG AAAGCCAGTC TTGAGGGCAT TCGCATGAAC
    TCGGAGGATC AATTGTGTCT GAGCCCGAGC ACCTCTCTGA GCTCCATCAT CCGCACGCCC
    AAATGCTACC ACATCTCCTC TGTGAACGAG AATGCAGCGA AGCGCTTGTG TCGCCGCTAC
    TCGCAGAAGC TGATCCAGCA CACCAGCTGC CAGCTGCTGC GCACGTACCC AGCGGCCACA
    CGCATTGACT CTGCCAACCC CAATCCGCTC ATCTTCTGGC TGCACGGAGT CCAGCTGGTG
    GCCCTCAACT ACCAGACTGA TGATCTGCCG ATGCAGCTGA ACGCGGCTCT ATTCGAGGCT
    AATGGTCACT GTGGTTTTGT GCTGAAGCCT CCAGTCCTGT GGGACAGAAG CTGTCCTCTC
    TATCAGCACT TTTACCCTCT GGACCGAGAC CTGGAGAACA TGACCCCAAC GCTATACACA
    CTCACTATTG TGTCGGGGCA GAACGTGTGT CCAGGAAACT CAAACGGCAG TCCGTGTGTC
    GAAGTGGAGG TGTTGGGAAT GCCGGCGGAC AGCTGTCATT TCCGAACTAA ACCCATACAC
    CGCAACACAC TCAATCCCAT GTGGAACGAA CACTTCCAGT TCCACGTGCA CTTTGAAGAC
    CTGGCGTTCC TGCGCATCGC TGTGGTGGAG AACAACAGCT CTCAGGTCAC CGCTCAGAGG
    ATACTTCCCC TCAAAACACT CAGAGCAGGC TACCGGCACC TGCAGCTGAG GAACCTGCAC
    AACGAGCCTC TGGAGGTGTC CAGTCTGTTC ATGTTCAGCC GCAGGACGGA GGAGAGCCCG
    ACCGGAGGAC CACCGTCTGC GTCTGTGCTG TTCAGCACCG AGGAGAGACG ATCTGTGCAG
    CAGCACAAGG TGACCGTGCA TGGAGTCCCC GGACCTGAAC CCTTCACTGT GATCTGTGTC
    GATGAATTCA CCACAGCCAA ACAGCTCCTG GACTCTCTGT TTCCGACTGC CTCTTTTAAG
    TATATGCTGA TGGAGGAGAG AGTGTCTCTG TCGAAGGAGA AGAAGGCTCC TCAGCAGCGT
    CCTCTAGCCA ATGATGAGAG ACTGCTGAAG CTCATTCACA GCTGGCAGCC TGAGGACGGA
    TATGTGGGCA GAATATACCT CAAAACCAGA GAACAGCAGA ACTGCAGTGA GAAGACGAGC
    GTCCCGCTGG AGTCACTGGA GGAGCTCGGC AGCTTAGAGG ACGACACCTT CTTCGTGCAG
    GTCCATGATG TTTCTCCAGA ACAGCCTCAC ACCGTCATCA AAGCCCCGCG ATACAGCACA
    GCACAGGACA TCATCCAGCA GACTCTGAGC AAAGCCAAGT ATTCTCTGAG TATCCTGAGC
    AACCCCAACC CATGTGACTA CGTCCTGATG GAGGAGCTTT GTAAAGACGC GGGTGGAAAG
    AAGTCGTCCT CTGCCAAACC GTGCCAGCGT GTGCTGCAGG ACCACGAGTG TGTGTTTCAG
    GCTCAGAGCC GCTGGAAGGG GGCTGGAAAA TTCATCCTCA AACTCAAAGA GCAGTTGGCT
    CGTGAGGACA AGCGTAAAGG TGTGTCGTTC GCCAGTGAGC TGAGGAAGCT GACGGGTCGC
    AGTCGCAGTG TGACGGTCAA CACAAACAGC AACGACACTC ACAGGTCTCT GTCTCTGGGG
    CTGAAGGCCT GGATGGCTGA GGGCCGCTTG GGCTCTCCGA GACGCTCCCA GTGTGGTGAC
    GGTGTCTCGG CCTTGTCCGG GTGGTCTCTC CCGCTGCTGT GGAACTGGAA GCTGCATAAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335824.1
    CDS432..7274
    Translation

    Target ORF information:

    RefSeq Version XM_021480149.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480149.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GTTTTCAATC
    GGCGACCTCT CAGACTCTGA AGGGGACCCA CTTGAGACAG TTAAGGATGT TGTAGACCTC
    CAGACCACCG AAGATGTTCG CGGACCCTTT AGCCATGGGA CTGAGCTCAT TCCCTGGTAC
    GTTCTGTCGC TTCAGCCGGA TATCCATCAG TTCCTGCTCC AAGGAGCAAC CGTCATCCAC
    TACGACCCCG AGAGCCACCT CACCGCCCGC TGTCTTCTCC GTCTACAGCC CGATAACTGC
    TTCCTCACCT GGTGCAAACC CCACAGTAGC TGCAGCCTAT ATGGAAGAGC TAGAACCTTC
    ATGGGACATC CAGCATCCCC AGACCACTTA CACATCGGCC AACCTGTTCA CTGCGGACTC
    TCTGACGGGC TCCTGGATCT TAATGTGGTG AAGGCTGTGT TTATGGGTCA CCCTGGAGTG
    GACGTCAACT ATGTCTGCCT GCAGCACAAA CTGTGTAACA TGAATCCTGG TGAAAATGGT
    GTCACGCTGC TATATGGACT TCACACCACA GACAACAGGC TCCTGCACTT TGTGGCCCCG
    AAACACACGG CTCGCATGCT GCACGAGGGC CTCCAGGAGC TGCTGAACTC TATTAGGAAG
    ATCAGGAAAT TCCCTGACCA GAGACTGCAA TGGCTGAGAA AGCAATATGT TAGTTTGTAC
    CAGGAGGATG GTAGATTTGA AGGACCTACA CTGGCTCATG CTATCGAGCT GTTCGGAGGG
    AGACGCTGGA ATATGGGAAC TAGCGGCCCT GGATCCGCCA GCAGAGGTGC AGAGAAGAAC
    TCGGCTCAGA AGAACAGTCC ACTGGGCATC AACTCCAATG TCAAAAAAAA GAAAAAGGCC
    TTAGTCAGGG GGGACAGTGG CGATGGAACT GATGATGAGA TGACCGCACG CAAGACCAGG
    AGTTGCAAAG AGACTTTGGG GAGGAGAGAG TCAGATGCTC TGGAAAATGT TGAGCAGGAA
    GAGGCTGAGG ACTGCAGTAC CTTTAGCCAA CCTGGACCAT TTTCCTTATC CAACAACAAA
    GGCCAGTCTC CGGGATCCTC CTCGTCCTCC TCCACCTCTT CTTCTGTTGC CTCCTTTTGC
    ACAAACTCCT CCACCCCTAC ACGTCCACAT TCCTCCCCAA TCCTCCCCAC AAACCTCAAG
    AGTCAGCCAG GGGCCTGGAG CAGTCGAAGC TGGCACGGTC GAGGGAAAGG CTGCTTCAGA
    GGTTTCCAGG ACCTCATGAT CTCCGACAGC ATCATGAGCT TTGTCGAATT TGTGGAGCTC
    TTCAAATCTT TTAGCATTCG CAGTCGTAAG GATCTGAAGG AACTATTCGA CACCTACGCC
    GTGCCCTGCA GTCGCTCTGG CCCTGAGTCT GTCCCCCTCT ACACCACACT TAGGATTGAC
    GACAAGCTAA CCGGACTGCA GCCAGATCTG GATCTCCTCA CTCGCAACGG CTCTGACCTG
    GGCCTTTTCA TTCGCACCCG CCAGCAAATG TCTGAAAACC AGAAGCAGAT TTCAGACGCC
    ATCGCAGCTG CCAGTATTGT GACCAATGGC ACTGGAGTGG AGAATTCATC GCTGGGTGTG
    CTGGGACTGG CCATCTCGCA GCTCAATGAT TTCTTGGTCA ACTGTCAGAG AGAACATCTG
    AGCTATGATG AGATCCTCAG CATCATACAG AAATTTGAGC CTTCTTCAAA CATGCGTCAG
    ATGGGGTGGA TGTCGTTTGA AGGATTCGCC AGGTTTCTAA TGGACAAGGA CAACTTTGCC
    TCCAAAAATG AGGAGTCTCA AGTGAACCTG GATGAGCTCC AGCATCCGCT GTCTTACTAT
    TACATCGAGT CCTCTCATAA CACATACCTG ACGGGGCATC AGCTGAAGGG AGAGTCTTCA
    GTCGAGCTCT ACAGCCAGGT GTTGCTGCAG GGTTGTCGCA GCGTTGAGTT GGACTGCTGG
    GATGGGGATG ACGGCATGCC TGTTATTTAC CATGGACATA CTCTTACCAC TAAGATTCCT
    TTCAAGGATG TTGTAGAGGC CATCAACCGT GCAGCATTTG TGAACTCAGA GATGCCTGTG
    ATTCTGTCCA TTGAAAACCA CTGCTCCTTA CCACAGCAGC GCAAAATGGC TGAGATTTTT
    AAGATGGTGT TTGGTGAGAA GCTGGTGACC AAGTTTCTGT TTGAAAGTGA TTTCGCGGAC
    GAGCCTTTGC TTCCGTCTCC CCTTCAGCTC CGGGGGAAAA TCCTCCTGAA GAATAAAAAG
    TTAAAAGCTC ACCAGGCTCC TGTTGACATT CTCAAACAAA AGGCGCATCA GCTTGCCCAC
    ATGCAGGCTC AGGCCAACAA CGGCACAGTC AGCACAACGC CACTGGGAAA CAATGATGAA
    GAGGAGGAGG AGGAAGACGA GTACGATTAC GATTACGAGT CGCTGTCTGA CGCTGATGTC
    CTCAATGCTT CCACAGCCTC TTATGGCCAG GAAGACAATA TTCTGGATGA CAAACCTGAA
    GGCAAGTCAT CTACGGAGAA ACTTCAGTAC GAGTCCAACG ATGAGATGCC AAAGCGGTTT
    AAAAAAGCTG GAAGCAAAGG AAAGATGTTT GACATGGAGC TGGGAGAGGA GTTTTACCTG
    CCCCAGAACG AGAAGGAGAG TAGACAGATT GCTCAAGAGC TGTCAGATCT GGTCATCTAC
    TGCCAGGCTA TCAAATTTCC AGGCCTCTCC ACCCTCTCTC CATCAGGCTC TGGAAGGGGG
    AAAGACAGAA AGAGCCGGAA ATCCATCTTT GGCAGCGCTC CTGCTCGCGG GAGCCCCGGG
    GAGCCTGTGA CGCTGGTCCG AGCTCCAGGG AAAGCCAGTC TTGAGGGCAT TCGCATGAAC
    TCGGAGGATC AATTGTGTCT GAGCCCGAGC ACCTCTCTGA GCTCCATCAT CCGCACGCCC
    AAATGCTACC ACATCTCCTC TGTGAACGAG AATGCAGCGA AGCGCTTGTG TCGCCGCTAC
    TCGCAGAAGC TGATCCAGCA CACCAGCTGC CAGCTGCTGC GCACGTACCC AGCGGCCACA
    CGCATTGACT CTGCCAACCC CAATCCGCTC ATCTTCTGGC TGCACGGAGT CCAGCTGGTG
    GCCCTCAACT ACCAGACTGA TGATCTGCCG ATGCAGCTGA ACGCGGCTCT ATTCGAGGCT
    AATGGTCACT GTGGTTTTGT GCTGAAGCCT CCAGTCCTGT GGGACAGAAG CTGTCCTCTC
    TATCAGCACT TTTACCCTCT GGACCGAGAC CTGGAGAACA TGACCCCAAC GCTATACACA
    CTCACTATTG TGTCGGGGCA GAACGTGTGT CCAGGAAACT CAAACGGCAG TCCGTGTGTC
    GAAGTGGAGG TGTTGGGAAT GCCGGCGGAC AGCTGTCATT TCCGAACTAA ACCCATACAC
    CGCAACACAC TCAATCCCAT GTGGAACGAA CACTTCCAGT TCCACGTGCA CTTTGAAGAC
    CTGGCGTTCC TGCGCATCGC TGTGGTGGAG AACAACAGCT CTCAGGTCAC CGCTCAGAGG
    ATACTTCCCC TCAAAACACT CAGAGCAGGC TACCGGCACC TGCAGCTGAG GAACCTGCAC
    AACGAGCCTC TGGAGGTGTC CAGTCTGTTC ATGTTCAGCC GCAGGACGGA GGAGAGCCCG
    ACCGGAGGAC CACCGTCTGC GTCTGTGCTG TTCAGCACCG AGGAGAGACG ATCTGTGCAG
    CAGCACAAGG TGACCGTGCA TGGAGTCCCC GGACCTGAAC CCTTCACTGT GATCTGTGTC
    GATGAATTCA CCACAGCCAA ACAGCTCCTG GACTCTCTGT TTCCGACTGC CTCTTTTAAG
    TATATGCTGA TGGAGGAGAG AGTGTCTCTG TCGAAGGAGA AGAAGGCTCC TCAGCAGCGT
    CCTCTAGCCA ATGATGAGAG ACTGCTGAAG CTCATTCACA GCTGGCAGCC TGAGGACGGA
    TATGTGGGCA GAATATACCT CAAAACCAGA GAACAGCAGA ACTGCAGTGA GAAGACGAGC
    GTCCCGCTGG AGTCACTGGA GGAGCTCGGC AGCTTAGAGG ACGACACCTT CTTCGTGCAG
    GTCCATGATG TTTCTCCAGA ACAGCCTCAC ACCGTCATCA AAGCCCCGCG ATACAGCACA
    GCACAGGACA TCATCCAGCA GACTCTGAGC AAAGCCAAGT ATTCTCTGAG TATCCTGAGC
    AACCCCAACC CATGTGACTA CGTCCTGATG GAGGAGCTTT GTAAAGACGC GGGTGGAAAG
    AAGTCGTCCT CTGCCAAACC GTGCCAGCGT GTGCTGCAGG ACCACGAGTG TGTGTTTCAG
    GCTCAGAGCC GCTGGAAGGG GGCTGGAAAA TTCATCCTCA AACTCAAAGA GCAGTTGGCT
    CGTGAGGACA AGCGTAAAGG TGTGTCGTTC GCCAGTGAGC TGAGGAAGCT GACGGGTCGC
    AGTCGCAGTG TGACGGTCAA CACAAACAGC AACGACACTC ACAGGTCTCT GTCTCTGGGG
    CTGAAGGCCT GGATGGCTGA GGGCCGCTTG GGCTCTCCGA GACGCTCCCA GTGTGGTGAC
    GGTGTCTCGG CCTTGTCCGG GTGGTCTCTC CCGCTGCTGT GGAACTGGAA GCTGCATAAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148223
    Clone ID Related Accession (Same CDS sequence) XM_021480150.1
    Accession Version XM_021480150.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GAAAGGACCT
    GGGACTGAAC GAGTCCTGTT TTCAATCGGC GACCTCTCAG ACTCTGAAGG GGACCCACTT
    GAGACAGTTA AGGATGTTGT AGACCTCCAG ACCACCGAAG ATGTTCGCGG ACCCTTTAGC
    CATGGGACTG AGCTCATTCC CTGGTACGTT CTGTCGCTTC AGCCGGATAT CCATCAGTTC
    CTGCTCCAAG GAGCAACCGT CATCCACTAC GACCCCGAGA GCCACCTCAC CGCCCGCTGT
    CTTCTCCGTC TACAGCCCGA TAACTGCTTC CTCACCTGGT GCAAACCCCA CAGTAGCTGC
    AGCCTATATG GAAGAGCTAG AACCTTCATG GGACATCCAG CATCCCCAGA CCACTTACAC
    ATCGGCCAAC CTGTTCACTG CGGACTCTCT GACGGGCTCC TGGATCTTAA TGTGGTGAAG
    GCTGTGTTTA TGGGTCACCC TGGAGTGGAC GTCAACTATG TCTGCCTGCA GCACAAACTG
    TGTAACATGA ATCCTGGTGA AAATGGTGTC ACGCTGCTAT ATGGACTTCA CACCACAGAC
    AACAGGCTCC TGCACTTTGT GGCCCCGAAA CACACGGCTC GCATGCTGCA CGAGGGCCTC
    CAGGAGCTGC TGAACTCTAT TAGGAAGATC AGGAAATTCC CTGACCAGAG ACTGCAATGG
    CTGAGAAAGC AATATGTTAG TTTGTACCAG GAGGATGGTA GATTTGAAGG ACCTACACTG
    GCTCATGCTA TCGAGCTGTT CGGAGGGAGA CGCTGGAATA TGGGAACTAG CGGCCCTGGA
    TCCGCCAGCA GAGGTGCAGA GAAGAACTCG GCTCAGAAGA ACAGTCCACT GGGCATCAAC
    TCCAATGTCA AAAAAAAGAA AAAGGCCTTA GTCAGGGGGG ACAGTGGCGA TGGAACTGAT
    GATGAGATGA CCGCACGCAA GACCAGGAGT TGCAAAGAGA CTTTGGGGAG GAGAGAGTCA
    GATGCTCTGG AAAATGTTGA GCAGGAAGAG GCTGAGGACT GCAGTACCTT TAGCCAACCT
    GGACCATTTT CCTTATCCAA CAACAAAGGC CAGTCTCCGG GATCCTCCTC GTCCTCCTCC
    ACCTCTTCTT CTGTTGCCTC CTTTTGCACA AACTCCTCCA CCCCTACACG TCCACATTCC
    TCCCCAATCC TCCCCACAAA CCTCAAGAGT CAGCCAGGGG CCTGGAGCAG TCGAAGCTGG
    CACGGTCGAG GGAAAGGCTG CTTCAGAGGT TTCCAGGACC TCATGATCTC CGACAGCATC
    ATGAGCTTTG TCGAATTTGT GGAGCTCTTC AAATCTTTTA GCATTCGCAG TCGTAAGGAT
    CTGAAGGAAC TATTCGACAC CTACGCCGTG CCCTGCAGTC GCTCTGGCCC TGAGTCTGTC
    CCCCTCTACA CCACACTTAG GATTGACGAC AAGCTAACCG GACTGCAGCC AGATCTGGAT
    CTCCTCACTC GCAACGGCTC TGACCTGGGC CTTTTCATTC GCACCCGCCA GCAAATGTCT
    GAAAACCAGA AGCAGATTTC AGACGCCATC GCAGCTGCCA GTATTGTGAC CAATGGCACT
    GGAGTGGAGA ATTCATCGCT GGGTGTGCTG GGACTGGCCA TCTCGCAGCT CAATGATTTC
    TTGGTCAACT GTCAGAGAGA ACATCTGAGC TATGATGAGA TCCTCAGCAT CATACAGAAA
    TTTGAGCCTT CTTCAAACAT GCGTCAGATG GGGTGGATGT CGTTTGAAGG ATTCGCCAGG
    TTTCTAATGG ACAAGGACAA CTTTGCCTCC AAAAATGAGG AGTCTCAAGT GAACCTGGAT
    GAGCTCCAGC ATCCGCTGTC TTACTATTAC ATCGAGTCCT CTCATAACAC ATACCTGACG
    GGGCATCAGC TGAAGGGAGA GTCTTCAGTC GAGCTCTACA GCCAGGTGTT GCTGCAGGGT
    TGTCGCAGCG TTGAGTTGGA CTGCTGGGAT GGGGATGACG GCATGCCTGT TATTTACCAT
    GGACATACTC TTACCACTAA GATTCCTTTC AAGGATGTTG TAGAGGCCAT CAACCGTGCA
    GCATTTGTGA ACTCAGAGAT GCCTGTGATT CTGTCCATTG AAAACCACTG CTCCTTACCA
    CAGCAGCGCA AAATGGCTGA GATTTTTAAG ATGGTGTTTG GTGAGAAGCT GGTGACCAAG
    TTTCTGTTTG AAAGTGATTT CGCGGACGAG CCTTTGCTTC CGTCTCCCCT TCAGCTCCGG
    GGGAAAATCC TCCTGAAGAA TAAAAAGTTA AAAGCTCACC AGGCTCCTGT TGACATTCTC
    AAACAAAAGG CGCATCAGCT TGCCCACATG CAGGCTCAGG CCAACAACGG CACAGTCAGC
    ACAACGCCAC TGGGAAACAA TGATGAAGAG GAGGAGGAGG AAGACGAGTA CGATTACGAT
    TACGAGTCGC TGTCTGACGA CAATATTCTG GATGACAAAC CTGAAGGCAA GTCATCTACG
    GAGAAACTTC AGTACGAGTC CAACGATGAG ATGCCAAAGC GGTTTAAAAA AGCTGGAAGC
    AAAGGAAAGA TGTTTGACAT GGAGCTGGGA GAGGAGTTTT ACCTGCCCCA GAACGAGAAG
    GAGAGTAGAC AGATTGCTCA AGAGCTGTCA GATCTGGTCA TCTACTGCCA GGCTATCAAA
    TTTCCAGGCC TCTCCACCCT CTCTCCATCA GGCTCTGGAA GGGGGAAAGA CAGAAAGAGC
    CGGAAATCCA TCTTTGGCAG CGCTCCTGCT CGCGGGAGCC CCGGGGAGCC TGTGACGCTG
    GTCCGAGCTC CAGGGAAAGC CAGTCTTGAG GGCATTCGCA TGAACTCGGA GGATCAATTG
    TGTCTGAGCC CGAGCACCTC TCTGAGCTCC ATCATCCGCA CGCCCAAATG CTACCACATC
    TCCTCTGTGA ACGAGAATGC AGCGAAGCGC TTGTGTCGCC GCTACTCGCA GAAGCTGATC
    CAGCACACCA GCTGCCAGCT GCTGCGCACG TACCCAGCGG CCACACGCAT TGACTCTGCC
    AACCCCAATC CGCTCATCTT CTGGCTGCAC GGAGTCCAGC TGGTGGCCCT CAACTACCAG
    ACTGATGATC TGCCGATGCA GCTGAACGCG GCTCTATTCG AGGCTAATGG TCACTGTGGT
    TTTGTGCTGA AGCCTCCAGT CCTGTGGGAC AGAAGCTGTC CTCTCTATCA GCACTTTTAC
    CCTCTGGACC GAGACCTGGA GAACATGACC CCAACGCTAT ACACACTCAC TATTGTGTCG
    GGGCAGAACG TGTGTCCAGG AAACTCAAAC GGCAGTCCGT GTGTCGAAGT GGAGGTGTTG
    GGAATGCCGG CGGACAGCTG TCATTTCCGA ACTAAACCCA TACACCGCAA CACACTCAAT
    CCCATGTGGA ACGAACACTT CCAGTTCCAC GTGCACTTTG AAGACCTGGC GTTCCTGCGC
    ATCGCTGTGG TGGAGAACAA CAGCTCTCAG GTCACCGCTC AGAGGATACT TCCCCTCAAA
    ACACTCAGAG CAGGCTACCG GCACCTGCAG CTGAGGAACC TGCACAACGA GCCTCTGGAG
    GTGTCCAGTC TGTTCATGTT CAGCCGCAGG ACGGAGGAGA GCCCGACCGG AGGACCACCG
    TCTGCGTCTG TGCTGTTCAG CACCGAGGAG AGACGATCTG TGCAGCAGCA CAAGGTGACC
    GTGCATGGAG TCCCCGGACC TGAACCCTTC ACTGTGATCT GTGTCGATGA ATTCACCACA
    GCCAAACAGC TCCTGGACTC TCTGTTTCCG ACTGCCTCTT TTAAGTATAT GCTGATGGAG
    GAGAGAGTGT CTCTGTCGAA GGAGAAGAAG GCTCCTCAGC AGCGTCCTCT AGCCAATGAT
    GAGAGACTGC TGAAGCTCAT TCACAGCTGG CAGCCTGAGG ACGGATATGT GGGCAGAATA
    TACCTCAAAA CCAGAGAACA GCAGAACTGC AGTGAGAAGA CGAGCGTCCC GCTGGAGTCA
    CTGGAGGAGC TCGGCAGCTT AGAGGACGAC ACCTTCTTCG TGCAGGTCCA TGATGTTTCT
    CCAGAACAGC CTCACACCGT CATCAAAGCC CCGCGATACA GCACAGCACA GGACATCATC
    CAGCAGACTC TGAGCAAAGC CAAGTATTCT CTGAGTATCC TGAGCAACCC CAACCCATGT
    GACTACGTCC TGATGGAGGA GCTTTGTAAA GACGCGGGTG GAAAGAAGTC GTCCTCTGCC
    AAACCGTGCC AGCGTGTGCT GCAGGACCAC GAGTGTGTGT TTCAGGCTCA GAGCCGCTGG
    AAGGGGGCTG GAAAATTCAT CCTCAAACTC AAAGAGCAGT TGGCTCGTGA GGACAAGCGT
    AAAGGTGTGT CGTTCGCCAG TGAGCTGAGG AAGCTGACGG GTCGCAGTCG CAGTGTGACG
    GTCAACACAA ACAGCAACGA CACTCACAGG TCTCTGTCTC TGGGGCTGAA GGCCTGGATG
    GCTGAGGGCC GCTTGGGCTC TCCGAGACGC TCCCAGTGTG GTGACGGTGT CTCGGCCTTG
    TCCGGGTGGT CTCTCCCGCT GCTGTGGAAC TGGAAGCTGC ATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335825.1
    CDS432..7259
    Translation

    Target ORF information:

    RefSeq Version XM_021480150.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480150.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GAAAGGACCT
    GGGACTGAAC GAGTCCTGTT TTCAATCGGC GACCTCTCAG ACTCTGAAGG GGACCCACTT
    GAGACAGTTA AGGATGTTGT AGACCTCCAG ACCACCGAAG ATGTTCGCGG ACCCTTTAGC
    CATGGGACTG AGCTCATTCC CTGGTACGTT CTGTCGCTTC AGCCGGATAT CCATCAGTTC
    CTGCTCCAAG GAGCAACCGT CATCCACTAC GACCCCGAGA GCCACCTCAC CGCCCGCTGT
    CTTCTCCGTC TACAGCCCGA TAACTGCTTC CTCACCTGGT GCAAACCCCA CAGTAGCTGC
    AGCCTATATG GAAGAGCTAG AACCTTCATG GGACATCCAG CATCCCCAGA CCACTTACAC
    ATCGGCCAAC CTGTTCACTG CGGACTCTCT GACGGGCTCC TGGATCTTAA TGTGGTGAAG
    GCTGTGTTTA TGGGTCACCC TGGAGTGGAC GTCAACTATG TCTGCCTGCA GCACAAACTG
    TGTAACATGA ATCCTGGTGA AAATGGTGTC ACGCTGCTAT ATGGACTTCA CACCACAGAC
    AACAGGCTCC TGCACTTTGT GGCCCCGAAA CACACGGCTC GCATGCTGCA CGAGGGCCTC
    CAGGAGCTGC TGAACTCTAT TAGGAAGATC AGGAAATTCC CTGACCAGAG ACTGCAATGG
    CTGAGAAAGC AATATGTTAG TTTGTACCAG GAGGATGGTA GATTTGAAGG ACCTACACTG
    GCTCATGCTA TCGAGCTGTT CGGAGGGAGA CGCTGGAATA TGGGAACTAG CGGCCCTGGA
    TCCGCCAGCA GAGGTGCAGA GAAGAACTCG GCTCAGAAGA ACAGTCCACT GGGCATCAAC
    TCCAATGTCA AAAAAAAGAA AAAGGCCTTA GTCAGGGGGG ACAGTGGCGA TGGAACTGAT
    GATGAGATGA CCGCACGCAA GACCAGGAGT TGCAAAGAGA CTTTGGGGAG GAGAGAGTCA
    GATGCTCTGG AAAATGTTGA GCAGGAAGAG GCTGAGGACT GCAGTACCTT TAGCCAACCT
    GGACCATTTT CCTTATCCAA CAACAAAGGC CAGTCTCCGG GATCCTCCTC GTCCTCCTCC
    ACCTCTTCTT CTGTTGCCTC CTTTTGCACA AACTCCTCCA CCCCTACACG TCCACATTCC
    TCCCCAATCC TCCCCACAAA CCTCAAGAGT CAGCCAGGGG CCTGGAGCAG TCGAAGCTGG
    CACGGTCGAG GGAAAGGCTG CTTCAGAGGT TTCCAGGACC TCATGATCTC CGACAGCATC
    ATGAGCTTTG TCGAATTTGT GGAGCTCTTC AAATCTTTTA GCATTCGCAG TCGTAAGGAT
    CTGAAGGAAC TATTCGACAC CTACGCCGTG CCCTGCAGTC GCTCTGGCCC TGAGTCTGTC
    CCCCTCTACA CCACACTTAG GATTGACGAC AAGCTAACCG GACTGCAGCC AGATCTGGAT
    CTCCTCACTC GCAACGGCTC TGACCTGGGC CTTTTCATTC GCACCCGCCA GCAAATGTCT
    GAAAACCAGA AGCAGATTTC AGACGCCATC GCAGCTGCCA GTATTGTGAC CAATGGCACT
    GGAGTGGAGA ATTCATCGCT GGGTGTGCTG GGACTGGCCA TCTCGCAGCT CAATGATTTC
    TTGGTCAACT GTCAGAGAGA ACATCTGAGC TATGATGAGA TCCTCAGCAT CATACAGAAA
    TTTGAGCCTT CTTCAAACAT GCGTCAGATG GGGTGGATGT CGTTTGAAGG ATTCGCCAGG
    TTTCTAATGG ACAAGGACAA CTTTGCCTCC AAAAATGAGG AGTCTCAAGT GAACCTGGAT
    GAGCTCCAGC ATCCGCTGTC TTACTATTAC ATCGAGTCCT CTCATAACAC ATACCTGACG
    GGGCATCAGC TGAAGGGAGA GTCTTCAGTC GAGCTCTACA GCCAGGTGTT GCTGCAGGGT
    TGTCGCAGCG TTGAGTTGGA CTGCTGGGAT GGGGATGACG GCATGCCTGT TATTTACCAT
    GGACATACTC TTACCACTAA GATTCCTTTC AAGGATGTTG TAGAGGCCAT CAACCGTGCA
    GCATTTGTGA ACTCAGAGAT GCCTGTGATT CTGTCCATTG AAAACCACTG CTCCTTACCA
    CAGCAGCGCA AAATGGCTGA GATTTTTAAG ATGGTGTTTG GTGAGAAGCT GGTGACCAAG
    TTTCTGTTTG AAAGTGATTT CGCGGACGAG CCTTTGCTTC CGTCTCCCCT TCAGCTCCGG
    GGGAAAATCC TCCTGAAGAA TAAAAAGTTA AAAGCTCACC AGGCTCCTGT TGACATTCTC
    AAACAAAAGG CGCATCAGCT TGCCCACATG CAGGCTCAGG CCAACAACGG CACAGTCAGC
    ACAACGCCAC TGGGAAACAA TGATGAAGAG GAGGAGGAGG AAGACGAGTA CGATTACGAT
    TACGAGTCGC TGTCTGACGA CAATATTCTG GATGACAAAC CTGAAGGCAA GTCATCTACG
    GAGAAACTTC AGTACGAGTC CAACGATGAG ATGCCAAAGC GGTTTAAAAA AGCTGGAAGC
    AAAGGAAAGA TGTTTGACAT GGAGCTGGGA GAGGAGTTTT ACCTGCCCCA GAACGAGAAG
    GAGAGTAGAC AGATTGCTCA AGAGCTGTCA GATCTGGTCA TCTACTGCCA GGCTATCAAA
    TTTCCAGGCC TCTCCACCCT CTCTCCATCA GGCTCTGGAA GGGGGAAAGA CAGAAAGAGC
    CGGAAATCCA TCTTTGGCAG CGCTCCTGCT CGCGGGAGCC CCGGGGAGCC TGTGACGCTG
    GTCCGAGCTC CAGGGAAAGC CAGTCTTGAG GGCATTCGCA TGAACTCGGA GGATCAATTG
    TGTCTGAGCC CGAGCACCTC TCTGAGCTCC ATCATCCGCA CGCCCAAATG CTACCACATC
    TCCTCTGTGA ACGAGAATGC AGCGAAGCGC TTGTGTCGCC GCTACTCGCA GAAGCTGATC
    CAGCACACCA GCTGCCAGCT GCTGCGCACG TACCCAGCGG CCACACGCAT TGACTCTGCC
    AACCCCAATC CGCTCATCTT CTGGCTGCAC GGAGTCCAGC TGGTGGCCCT CAACTACCAG
    ACTGATGATC TGCCGATGCA GCTGAACGCG GCTCTATTCG AGGCTAATGG TCACTGTGGT
    TTTGTGCTGA AGCCTCCAGT CCTGTGGGAC AGAAGCTGTC CTCTCTATCA GCACTTTTAC
    CCTCTGGACC GAGACCTGGA GAACATGACC CCAACGCTAT ACACACTCAC TATTGTGTCG
    GGGCAGAACG TGTGTCCAGG AAACTCAAAC GGCAGTCCGT GTGTCGAAGT GGAGGTGTTG
    GGAATGCCGG CGGACAGCTG TCATTTCCGA ACTAAACCCA TACACCGCAA CACACTCAAT
    CCCATGTGGA ACGAACACTT CCAGTTCCAC GTGCACTTTG AAGACCTGGC GTTCCTGCGC
    ATCGCTGTGG TGGAGAACAA CAGCTCTCAG GTCACCGCTC AGAGGATACT TCCCCTCAAA
    ACACTCAGAG CAGGCTACCG GCACCTGCAG CTGAGGAACC TGCACAACGA GCCTCTGGAG
    GTGTCCAGTC TGTTCATGTT CAGCCGCAGG ACGGAGGAGA GCCCGACCGG AGGACCACCG
    TCTGCGTCTG TGCTGTTCAG CACCGAGGAG AGACGATCTG TGCAGCAGCA CAAGGTGACC
    GTGCATGGAG TCCCCGGACC TGAACCCTTC ACTGTGATCT GTGTCGATGA ATTCACCACA
    GCCAAACAGC TCCTGGACTC TCTGTTTCCG ACTGCCTCTT TTAAGTATAT GCTGATGGAG
    GAGAGAGTGT CTCTGTCGAA GGAGAAGAAG GCTCCTCAGC AGCGTCCTCT AGCCAATGAT
    GAGAGACTGC TGAAGCTCAT TCACAGCTGG CAGCCTGAGG ACGGATATGT GGGCAGAATA
    TACCTCAAAA CCAGAGAACA GCAGAACTGC AGTGAGAAGA CGAGCGTCCC GCTGGAGTCA
    CTGGAGGAGC TCGGCAGCTT AGAGGACGAC ACCTTCTTCG TGCAGGTCCA TGATGTTTCT
    CCAGAACAGC CTCACACCGT CATCAAAGCC CCGCGATACA GCACAGCACA GGACATCATC
    CAGCAGACTC TGAGCAAAGC CAAGTATTCT CTGAGTATCC TGAGCAACCC CAACCCATGT
    GACTACGTCC TGATGGAGGA GCTTTGTAAA GACGCGGGTG GAAAGAAGTC GTCCTCTGCC
    AAACCGTGCC AGCGTGTGCT GCAGGACCAC GAGTGTGTGT TTCAGGCTCA GAGCCGCTGG
    AAGGGGGCTG GAAAATTCAT CCTCAAACTC AAAGAGCAGT TGGCTCGTGA GGACAAGCGT
    AAAGGTGTGT CGTTCGCCAG TGAGCTGAGG AAGCTGACGG GTCGCAGTCG CAGTGTGACG
    GTCAACACAA ACAGCAACGA CACTCACAGG TCTCTGTCTC TGGGGCTGAA GGCCTGGATG
    GCTGAGGGCC GCTTGGGCTC TCCGAGACGC TCCCAGTGTG GTGACGGTGT CTCGGCCTTG
    TCCGGGTGGT CTCTCCCGCT GCTGTGGAAC TGGAAGCTGC ATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148224
    Clone ID Related Accession (Same CDS sequence) XM_021480151.1
    Accession Version XM_021480151.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GAAAGGACCT
    GGGACTGAAC GAGTCCTGTT TTCAATCGGC GACCTCTCAG ACTCTGAAGG GGACCCACTT
    GAGACAGTTA AGGATGTTGT AGACCTCCAG ACCACCGAAG ATGTTCGCGG ACCCTTTAGC
    CATGGGACTG AGCTCATTCC CTGGTACGTT CTGTCGCTTC AGCCGGATAT CCATCAGTTC
    CTGCTCCAAG GAGCAACCGT CATCCACTAC GACCCCGAGA GCCACCTCAC CGCCCGCTGT
    CTTCTCCGTC TACAGCCCGA TAACTGCTTC CTCACCTGGT GCAAACCCCA CAGTAGCTGC
    AGCCTATATG GAAGAGCTAG AACCTTCATG GGACATCCAG CATCCCCAGA CCACTTACAC
    ATCGGCCAAC CTGTTCACTG CGGACTCTCT GACGGGCTCC TGGATCTTAA TGTGGTGAAG
    GCTGTGTTTA TGGGTCACCC TGGAGTGGAC GTCAACTATG TCTGCCTGCA GCACAAACTG
    TGTAACATGA ATCCTGGTGA AAATGGTGTC ACGCTGCTAT ATGGACTTCA CACCACAGAC
    AACAGGCTCC TGCACTTTGT GGCCCCGAAA CACACGGCTC GCATGCTGCA CGAGGGCCTC
    CAGGAGCTGC TGAACTCTAT TAGGAAGATC AGGAAATTCC CTGACCAGAG ACTGCAATGG
    CTGAGAAAGC AATATGTTAG TTTGTACCAG GAGGATGGTA GATTTGAAGG ACCTACACTG
    GCTCATGCTA TCGAGCTGTT CGGAGGGAGA CGCTGGAATA TGGGAACTAG CGGCCCTGGA
    TCCGCCAGCA GAGGTGCAGA GAAGAACTCG GCTCAGAAGA ACAGTCCACT GGGCATCAAC
    TCCAATGTCA AAAAAAAGAA AAAGGCCTTA GTCAGGGGGG ACAGTGGCGA TGGAACTGAT
    GATGAGATGA CCGCACGCAA GACCAGGAGT TGCAAAGAGA CTTTGGGGAG GAGAGAGTCA
    GATGCTCTGG AAAATGTTGA GCAGGAAGAG GCTGAGGACT GCAGTACCTT TAGCCAACCT
    GGACCATTTT CCTTATCCAA CAACAAAGGC CAGTCTCCGG GATCCTCCTC GTCCTCCTCC
    ACCTCTTCTT CTGTTGCCTC CTTTTGCACA AACTCCTCCA CCCCTACACG TCCACATTCC
    TCCCCAATCC TCCCCACAAA CCTCAAGAGT CAGCCAGGGG CCTGGAGCAG TCGAAGCTGG
    CACGGTCGAG GGAAAGGCTG CTTCAGAGGT TTCCAGGACC TCATGATCTC CGACAGCATC
    ATGAGCTTTG TCGAATTTGT GGAGCTCTTC AAATCTTTTA GCATTCGCAG TCGTAAGGAT
    CTGAAGGAAC TATTCGACAC CTACGCCGTG CCCTGCAGTC GCTCTGGCCC TGAGTCTGTC
    CCCCTCTACA CCACACTTAG GATTGACGAC AAGCTAACCG GACTGCAGCC AGATCTGGAT
    CTCCTCACTC GCAACGGCTC TGACCTGGGC CTTTTCATTC GCACCCGCCA GCAAATGTCT
    GAAAACCAGA AGCAGATTTC AGACGCCATC GCAGCTGCCA GTATTGTGAC CAATGGCACT
    GGAGTGGAGA ATTCATCGCT GGGTGTGCTG GGACTGGCCA TCTCGCAGCT CAATGATTTC
    TTGGTCAACT GTCAGAGAGA ACATCTGAGC TATGATGAGA TCCTCAGCAT CATACAGAAA
    TTTGAGCCTT CTTCAAACAT GCGTCAGATG GGGTGGATGT CGTTTGAAGG ATTCGCCAGG
    TTTCTAATGG ACAAGGACAA CTTTGCCTCC AAAAATGAGG AGTCTCAAGT GAACCTGGAT
    GAGCTCCAGC ATCCGCTGTC TTACTATTAC ATCGAGTCCT CTCATAACAC ATACCTGACG
    GGGCATCAGC TGAAGGGAGA GTCTTCAGTC GAGCTCTACA GCCAGGTGTT GCTGCAGGGT
    TGTCGCAGCG TTGAGTTGGA CTGCTGGGAT GGGGATGACG GCATGCCTGT TATTTACCAT
    GGACATACTC TTACCACTAA GATTCCTTTC AAGGATGTTG TAGAGGCCAT CAACCGTGCA
    GCATTTGTGA ACTCAGAGAT GCCTGTGATT CTGTCCATTG AAAACCACTG CTCCTTACCA
    CAGCAGCGCA AAATGGCTGA GATTTTTAAG ATGGTGTTTG GTGAGAAGCT GGTGACCAAG
    TTTCTGTTTG AAAGTGATTT CGCGGACGAG CCTTTGCTTC CGTCTCCCCT TCAGCTCCGG
    GGGAAAATCC TCCTGAAGAA TAAAAAGTTA AAAGCTCACC AGGCTCCTGT TGACATTCTC
    AAACAAAAGG CGCATCAGCT TGCCCACATG CAGGCTCAGG CCAACAACGG CACAGTCAGC
    ACAACGCCAC TGGGAAACAA TGATGAAGAG GAGGAGGAGG AAGACGAGTA CGATTACGAT
    TACGAGTCGC TGTCTGACGC TGATGTCCTC AATGCTTCCA CAGCCTCTTA TGGCCAGGAA
    GACAATATTC TGGATGACAA ACCTGAAGGC AAGTCATCTA CGGAGAAACT TCAGTACGAG
    TCCAACGATG AGATGCCAAA GCGGTTTAAA AAAGCTGGAA GCAAAGGAAA GATGTTTGAC
    ATGGAGCTGG GAGAGGAGTT TTACCTGCCC CAGAACGAGA AGGAGAGTAG ACAGATTGCT
    CAAGAGCTGT CAGATCTGGT CATCTACTGC CAGGCTATCA AATTTCCAGG CCTCTCCACC
    CTCTCTCCAT CAGGCTCTGG AAGGGGGAAA GACAGAAAGA GCCGGAAATC CATCTTTGGC
    AGCGCTCCTG CTCGCGGGAG CCCCGGGGAG CCTGTGACGC TGGTCCGAGC TCCAGGGAAA
    GCCAGTCTTG AGGGCATTCG CATGAACTCG GAGGATCAAT TGTGTCTGAG CCCGAGCACC
    TCTCTGAGCT CCATCATCCG CACGCCCAAA TGCTACCACA TCTCCTCTGT GAACGAGAAT
    GCAGCGAAGC GCTTGTGTCG CCGCTACTCG CAGAAGCTGA TCCAGCACAC CAGCTGCCAG
    CTGCTGCGCA CGTACCCAGC GGCCACACGC ATTGACTCTG CCAACCCCAA TCCGCTCATC
    TTCTGGCTGC ACGGAGTCCA GCTGGTGGCC CTCAACTACC AGACTGATGA TCTGCCGATG
    CAGCTGAACG CGGCTCTATT CGAGGCTAAT GGTCACTGTG GTTTTGTGCT GAAGCCTCCA
    GTCCTGTGGG ACAGAAGCTG TCCTCTCTAT CAGCACTTTT ACCCTCTGGA CCGAGACCTG
    GAGAACATGA CCCCAACGCT ATACACACTC ACTATTGTGT CGGGGCAGAA CGTGTGTCCA
    GGAAACTCAA ACGGCAGTCC GTGTGTCGAA GTGGAGGTGT TGGGAATGCC GGCGGACAGC
    TGTCATTTCC GAACTAAACC CATACACCGC AACACACTCA ATCCCATGTG GAACGAACAC
    TTCCAGTTCC ACGTGCACTT TGAAGACCTG GCGTTCCTGC GCATCGCTGT GGTGGAGAAC
    AACAGCTCTC AGGTCACCGC TCAGAGGATA CTTCCCCTCA AAACACTCAG AGCAGGCTAC
    CGGCACCTGC AGCTGAGGAA CCTGCACAAC GAGCCTCTGG AGGTGTCCAG TCTGTTCATG
    TTCAGCCGCA GGACGGAGGA GAGCCCGACC GGAGGACCAC CGTCTGCGTC TGTGCTGTTC
    AGCACCGAGG AGAGACGATC TGTGCAGCAG CACAAGGTGA CCGTGCATGG AGTCCCCGGA
    CCTGAACCCT TCACTGTGAT CTGTGTCGAT GAATTCACCA CAGCCAAACA GCTCCTGGAC
    TCTCTGTTTC CGACTGCCTC TTTTAAGTAT ATGCTGATGG AGGAGAGAGT GTCTCTGTCG
    AAGGAGAAGA AGGCTCCTCA GCAGCGTCCT CTAGCCAATG ATGAGAGACT GCTGAAGCTC
    ATTCACAGCT GGCAGCCTGA GGACGGATAT GTGGGCAGAA TATACCTCAA AACCAGAGAA
    CAGCAGAACT GCAGTGAGAA GACGAGCGTC CCGCTGGAGT CACTGGAGGA GCTCGGCAGC
    TTAGAGGACG ACACCTTCTT CGTGCAGGTC CATGATGTTT CTCCAGAACA GCCTCACACC
    GTCATCAAAG CCCCGCGATA CAGCACAGCA CAGGACATCA TCCAGCAGAC TCTGAGCAAA
    GCCAAGTATT CTCTGAGTAT CCTGAGCAAC CCCAACCCAT GTGACTACGT CCTGATGGAG
    GAGCTTTGTA AAGACGCGGG TGGAAAGAAG TCGTCCTCTG CCAAACCGTG CCAGCGTGTG
    CTGCAGGACC ACGAGTGTGT GTTTCAGGCT CAGAGCCGCT GGAAGGGGGC TGGAAAATTC
    ATCCTCAAAC TCAAAGAGCA GTTGGCTCGT GAGGACAAGC GTAAAGGTGT GTCGTTCGCC
    AGTGAGCTGA GGAAGCTGAC GGGTCGCAGT CGCAGTGTGA CGGTCAACAC AAACAGCAAC
    GACACTCACA GTAAGGAAGA GAAGAGCGCA TGTCCCAGCA TGAGCTGTGT GCCGGAGACA
    TCGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335826.1
    CDS432..7220
    Translation

    Target ORF information:

    RefSeq Version XM_021480151.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480151.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GAAAGGACCT
    GGGACTGAAC GAGTCCTGTT TTCAATCGGC GACCTCTCAG ACTCTGAAGG GGACCCACTT
    GAGACAGTTA AGGATGTTGT AGACCTCCAG ACCACCGAAG ATGTTCGCGG ACCCTTTAGC
    CATGGGACTG AGCTCATTCC CTGGTACGTT CTGTCGCTTC AGCCGGATAT CCATCAGTTC
    CTGCTCCAAG GAGCAACCGT CATCCACTAC GACCCCGAGA GCCACCTCAC CGCCCGCTGT
    CTTCTCCGTC TACAGCCCGA TAACTGCTTC CTCACCTGGT GCAAACCCCA CAGTAGCTGC
    AGCCTATATG GAAGAGCTAG AACCTTCATG GGACATCCAG CATCCCCAGA CCACTTACAC
    ATCGGCCAAC CTGTTCACTG CGGACTCTCT GACGGGCTCC TGGATCTTAA TGTGGTGAAG
    GCTGTGTTTA TGGGTCACCC TGGAGTGGAC GTCAACTATG TCTGCCTGCA GCACAAACTG
    TGTAACATGA ATCCTGGTGA AAATGGTGTC ACGCTGCTAT ATGGACTTCA CACCACAGAC
    AACAGGCTCC TGCACTTTGT GGCCCCGAAA CACACGGCTC GCATGCTGCA CGAGGGCCTC
    CAGGAGCTGC TGAACTCTAT TAGGAAGATC AGGAAATTCC CTGACCAGAG ACTGCAATGG
    CTGAGAAAGC AATATGTTAG TTTGTACCAG GAGGATGGTA GATTTGAAGG ACCTACACTG
    GCTCATGCTA TCGAGCTGTT CGGAGGGAGA CGCTGGAATA TGGGAACTAG CGGCCCTGGA
    TCCGCCAGCA GAGGTGCAGA GAAGAACTCG GCTCAGAAGA ACAGTCCACT GGGCATCAAC
    TCCAATGTCA AAAAAAAGAA AAAGGCCTTA GTCAGGGGGG ACAGTGGCGA TGGAACTGAT
    GATGAGATGA CCGCACGCAA GACCAGGAGT TGCAAAGAGA CTTTGGGGAG GAGAGAGTCA
    GATGCTCTGG AAAATGTTGA GCAGGAAGAG GCTGAGGACT GCAGTACCTT TAGCCAACCT
    GGACCATTTT CCTTATCCAA CAACAAAGGC CAGTCTCCGG GATCCTCCTC GTCCTCCTCC
    ACCTCTTCTT CTGTTGCCTC CTTTTGCACA AACTCCTCCA CCCCTACACG TCCACATTCC
    TCCCCAATCC TCCCCACAAA CCTCAAGAGT CAGCCAGGGG CCTGGAGCAG TCGAAGCTGG
    CACGGTCGAG GGAAAGGCTG CTTCAGAGGT TTCCAGGACC TCATGATCTC CGACAGCATC
    ATGAGCTTTG TCGAATTTGT GGAGCTCTTC AAATCTTTTA GCATTCGCAG TCGTAAGGAT
    CTGAAGGAAC TATTCGACAC CTACGCCGTG CCCTGCAGTC GCTCTGGCCC TGAGTCTGTC
    CCCCTCTACA CCACACTTAG GATTGACGAC AAGCTAACCG GACTGCAGCC AGATCTGGAT
    CTCCTCACTC GCAACGGCTC TGACCTGGGC CTTTTCATTC GCACCCGCCA GCAAATGTCT
    GAAAACCAGA AGCAGATTTC AGACGCCATC GCAGCTGCCA GTATTGTGAC CAATGGCACT
    GGAGTGGAGA ATTCATCGCT GGGTGTGCTG GGACTGGCCA TCTCGCAGCT CAATGATTTC
    TTGGTCAACT GTCAGAGAGA ACATCTGAGC TATGATGAGA TCCTCAGCAT CATACAGAAA
    TTTGAGCCTT CTTCAAACAT GCGTCAGATG GGGTGGATGT CGTTTGAAGG ATTCGCCAGG
    TTTCTAATGG ACAAGGACAA CTTTGCCTCC AAAAATGAGG AGTCTCAAGT GAACCTGGAT
    GAGCTCCAGC ATCCGCTGTC TTACTATTAC ATCGAGTCCT CTCATAACAC ATACCTGACG
    GGGCATCAGC TGAAGGGAGA GTCTTCAGTC GAGCTCTACA GCCAGGTGTT GCTGCAGGGT
    TGTCGCAGCG TTGAGTTGGA CTGCTGGGAT GGGGATGACG GCATGCCTGT TATTTACCAT
    GGACATACTC TTACCACTAA GATTCCTTTC AAGGATGTTG TAGAGGCCAT CAACCGTGCA
    GCATTTGTGA ACTCAGAGAT GCCTGTGATT CTGTCCATTG AAAACCACTG CTCCTTACCA
    CAGCAGCGCA AAATGGCTGA GATTTTTAAG ATGGTGTTTG GTGAGAAGCT GGTGACCAAG
    TTTCTGTTTG AAAGTGATTT CGCGGACGAG CCTTTGCTTC CGTCTCCCCT TCAGCTCCGG
    GGGAAAATCC TCCTGAAGAA TAAAAAGTTA AAAGCTCACC AGGCTCCTGT TGACATTCTC
    AAACAAAAGG CGCATCAGCT TGCCCACATG CAGGCTCAGG CCAACAACGG CACAGTCAGC
    ACAACGCCAC TGGGAAACAA TGATGAAGAG GAGGAGGAGG AAGACGAGTA CGATTACGAT
    TACGAGTCGC TGTCTGACGC TGATGTCCTC AATGCTTCCA CAGCCTCTTA TGGCCAGGAA
    GACAATATTC TGGATGACAA ACCTGAAGGC AAGTCATCTA CGGAGAAACT TCAGTACGAG
    TCCAACGATG AGATGCCAAA GCGGTTTAAA AAAGCTGGAA GCAAAGGAAA GATGTTTGAC
    ATGGAGCTGG GAGAGGAGTT TTACCTGCCC CAGAACGAGA AGGAGAGTAG ACAGATTGCT
    CAAGAGCTGT CAGATCTGGT CATCTACTGC CAGGCTATCA AATTTCCAGG CCTCTCCACC
    CTCTCTCCAT CAGGCTCTGG AAGGGGGAAA GACAGAAAGA GCCGGAAATC CATCTTTGGC
    AGCGCTCCTG CTCGCGGGAG CCCCGGGGAG CCTGTGACGC TGGTCCGAGC TCCAGGGAAA
    GCCAGTCTTG AGGGCATTCG CATGAACTCG GAGGATCAAT TGTGTCTGAG CCCGAGCACC
    TCTCTGAGCT CCATCATCCG CACGCCCAAA TGCTACCACA TCTCCTCTGT GAACGAGAAT
    GCAGCGAAGC GCTTGTGTCG CCGCTACTCG CAGAAGCTGA TCCAGCACAC CAGCTGCCAG
    CTGCTGCGCA CGTACCCAGC GGCCACACGC ATTGACTCTG CCAACCCCAA TCCGCTCATC
    TTCTGGCTGC ACGGAGTCCA GCTGGTGGCC CTCAACTACC AGACTGATGA TCTGCCGATG
    CAGCTGAACG CGGCTCTATT CGAGGCTAAT GGTCACTGTG GTTTTGTGCT GAAGCCTCCA
    GTCCTGTGGG ACAGAAGCTG TCCTCTCTAT CAGCACTTTT ACCCTCTGGA CCGAGACCTG
    GAGAACATGA CCCCAACGCT ATACACACTC ACTATTGTGT CGGGGCAGAA CGTGTGTCCA
    GGAAACTCAA ACGGCAGTCC GTGTGTCGAA GTGGAGGTGT TGGGAATGCC GGCGGACAGC
    TGTCATTTCC GAACTAAACC CATACACCGC AACACACTCA ATCCCATGTG GAACGAACAC
    TTCCAGTTCC ACGTGCACTT TGAAGACCTG GCGTTCCTGC GCATCGCTGT GGTGGAGAAC
    AACAGCTCTC AGGTCACCGC TCAGAGGATA CTTCCCCTCA AAACACTCAG AGCAGGCTAC
    CGGCACCTGC AGCTGAGGAA CCTGCACAAC GAGCCTCTGG AGGTGTCCAG TCTGTTCATG
    TTCAGCCGCA GGACGGAGGA GAGCCCGACC GGAGGACCAC CGTCTGCGTC TGTGCTGTTC
    AGCACCGAGG AGAGACGATC TGTGCAGCAG CACAAGGTGA CCGTGCATGG AGTCCCCGGA
    CCTGAACCCT TCACTGTGAT CTGTGTCGAT GAATTCACCA CAGCCAAACA GCTCCTGGAC
    TCTCTGTTTC CGACTGCCTC TTTTAAGTAT ATGCTGATGG AGGAGAGAGT GTCTCTGTCG
    AAGGAGAAGA AGGCTCCTCA GCAGCGTCCT CTAGCCAATG ATGAGAGACT GCTGAAGCTC
    ATTCACAGCT GGCAGCCTGA GGACGGATAT GTGGGCAGAA TATACCTCAA AACCAGAGAA
    CAGCAGAACT GCAGTGAGAA GACGAGCGTC CCGCTGGAGT CACTGGAGGA GCTCGGCAGC
    TTAGAGGACG ACACCTTCTT CGTGCAGGTC CATGATGTTT CTCCAGAACA GCCTCACACC
    GTCATCAAAG CCCCGCGATA CAGCACAGCA CAGGACATCA TCCAGCAGAC TCTGAGCAAA
    GCCAAGTATT CTCTGAGTAT CCTGAGCAAC CCCAACCCAT GTGACTACGT CCTGATGGAG
    GAGCTTTGTA AAGACGCGGG TGGAAAGAAG TCGTCCTCTG CCAAACCGTG CCAGCGTGTG
    CTGCAGGACC ACGAGTGTGT GTTTCAGGCT CAGAGCCGCT GGAAGGGGGC TGGAAAATTC
    ATCCTCAAAC TCAAAGAGCA GTTGGCTCGT GAGGACAAGC GTAAAGGTGT GTCGTTCGCC
    AGTGAGCTGA GGAAGCTGAC GGGTCGCAGT CGCAGTGTGA CGGTCAACAC AAACAGCAAC
    GACACTCACA GTAAGGAAGA GAAGAGCGCA TGTCCCAGCA TGAGCTGTGT GCCGGAGACA
    TCGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148225
    Clone ID Related Accession (Same CDS sequence) XM_021480152.1
    Accession Version XM_021480152.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6747bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GAAAGGACCT
    GGGACTGAAC GAGTCCTGTT TTCAATCGGC GACCTCTCAG ACTCTGAAGG GGACCCACTT
    GAGACAGTTA AGGATGTTGT AGACCTCCAG ACCACCGAAG ATGTTCGCGG ACCCTTTAGC
    CATGGGACTG AGCTCATTCC CTGGTACGTT CTGTCGCTTC AGCCGGATAT CCATCAGTTC
    CTGCTCCAAG GAGCAACCGT CATCCACTAC GACCCCGAGA GCCACCTCAC CGCCCGCTGT
    CTTCTCCGTC TACAGCCCGA TAACTGCTTC CTCACCTGGT GCAAACCCCA CAGTAGCTGC
    AGCCTATATG GAAGAGCTAG AACCTTCATG GGACATCCAG CATCCCCAGA CCACTTACAC
    ATCGGCCAAC CTGTTCACTG CGGACTCTCT GACGGGCTCC TGGATCTTAA TGTGGTGAAG
    GCTGTGTTTA TGGGTCACCC TGGAGTGGAC GTCAACTATG TCTGCCTGCA GCACAAACTG
    TGTAACATGA ATCCTGGTGA AAATGGTGTC ACGCTGCTAT ATGGACTTCA CACCACAGAC
    AACAGGCTCC TGCACTTTGT GGCCCCGAAA CACACGGCTC GCATGCTGCA CGAGGGCCTC
    CAGGAGCTGC TGAACTCTAT TAGGAAGATC AGGAAATTCC CTGACCAGAG ACTGCAATGG
    CTGAGAAAGC AATATGTTAG TTTGTACCAG GAGGATGGTA GATTTGAAGG ACCTACACTG
    GCTCATGCTA TCGAGCTGTT CGGAGGGAGA CGCTGGAATA TGGGAACTAG CGGCCCTGGA
    TCCGCCAGCA GAGGTGCAGA GAAGAACTCG GCTCAGAAGA ACAGTCCACT GGGCATCAAC
    TCCAATGTCA AAAAAAAGAA AAAGGCCTTA GTCAGGGGGG ACAGTGGCGA TGGAACTGAT
    GATGAGATGA CCGCACGCAA GACCAGGAGT TGCAAAGAGA CTTTGGGGAG GAGAGAGTCA
    GATGCTCTGG AAAATGTTGA GCAGGAAGAG GCTGAGGACT GCAGTACCTT TAGCCAACCT
    GGACCATTTT CCTTATCCAA CAACAAAGGC CAGTCTCCGG GATCCTCCTC GTCCTCCTCC
    ACCTCTTCTT CTGTTGCCTC CTTTTGCACA AACTCCTCCA CCCCTACACG TCCACATTCC
    TCCCCAATCC TCCCCACAAA CCTCAAGAGT CAGCCAGGGG CCTGGAGCAG TCGAAGCTGG
    CACGGTCGAG GGAAAGGCTG CTTCAGAGGT TTCCAGGACC TCATGATCTC CGACAGCATC
    ATGAGCTTTG TCGAATTTGT GGAGCTCTTC AAATCTTTTA GCATTCGCAG TCGTAAGGAT
    CTGAAGGAAC TATTCGACAC CTACGCCGTG CCCTGCAGTC GCTCTGGCCC TGAGTCTGTC
    CCCCTCTACA CCACACTTAG GATTGACGAC AAGCTAACCG GACTGCAGCC AGATCTGGAT
    CTCCTCACTC GCAACGGCTC TGACCTGGGC CTTTTCATTC GCACCCGCCA GCAAATGTCT
    GAAAACCAGA AGCAGATTTC AGACGCCATC GCAGCTGCCA GTATTGTGAC CAATGGCACT
    GGAGTGGAGA ATTCATCGCT GGGTGTGCTG GGACTGGCCA TCTCGCAGCT CAATGATTTC
    TTGGTCAACT GTCAGAGAGA ACATCTGAGC TATGATGAGA TCCTCAGCAT CATACAGAAA
    TTTGAGCCTT CTTCAAACAT GCGTCAGATG GGGTGGATGT CGTTTGAAGG ATTCGCCAGG
    TTTCTAATGG ACAAGGACAA CTTTGCCTCC AAAAATGAGG AGTCTCAAGT GAACCTGGAT
    GAGCTCCAGC ATCCGCTGTC TTACTATTAC ATCGAGTCCT CTCATAACAC ATACCTGACG
    GGGCATCAGC TGAAGGGAGA GTCTTCAGTC GAGCTCTACA GCCAGGTGTT GCTGCAGGGT
    TGTCGCAGCG TTGAGTTGGA CTGCTGGGAT GGGGATGACG GCATGCCTGT TATTTACCAT
    GGACATACTC TTACCACTAA GATTCCTTTC AAGGATGTTG TAGAGGCCAT CAACCGTGCA
    GCATTTGTGA ACTCAGAGAT GCCTGTGATT CTGTCCATTG AAAACCACTG CTCCTTACCA
    CAGCAGCGCA AAATGGCTGA GATTTTTAAG ATGGTGTTTG GTGAGAAGCT GGTGACCAAG
    TTTCTGTTTG AAAGTGATTT CGCGGACGAG CCTTTGCTTC CGTCTCCCCT TCAGCTCCGG
    GGGAAAATCC TCCTGAAGAA TAAAAAGTTA AAAGCTCACC AGGCTCCTGT TGACATTCTC
    AAACAAAAGG CGCATCAGCT TGCCCACATG CAGGCTCAGG CCAACAACGG CACAGTCAGC
    ACAACGCCAC TGGGAAACAA TGATGAAGAG GAGGAGGAGG AAGACGAGTA CGATTACGAT
    TACGAGTCGC TGTCTGACGA CAATATTCTG GATGACAAAC CTGAAGGCAA GTCATCTACG
    GAGAAACTTC AGTACGAGTC CAACGATGAG ATGCCAAAGC GGTTTAAAAA AGCTGGAAGC
    AAAGGAAAGA TGTTTGACAT GGAGCTGGGA GAGGAGTTTT ACCTGCCCCA GAACGAGAAG
    GAGAGTAGAC AGATTGCTCA AGAGCTGTCA GATCTGGTCA TCTACTGCCA GGCTATCAAA
    TTTCCAGGCC TCTCCACCCT CTCTCCATCA GGCTCTGGAA GGGGGAAAGA CAGAAAGAGC
    CGGAAATCCA TCTTTGGCAG CGCTCCTGCT CGCGGGAGCC CCGGGGAGCC TGTGACGCTG
    GTCCGAGCTC CAGGGAAAGC CAGTCTTGAG GGCATTCGCA TGAACTCGGA GGATCAATTG
    TGTCTGAGCC CGAGCACCTC TCTGAGCTCC ATCATCCGCA CGCCCAAATG CTACCACATC
    TCCTCTGTGA ACGAGAATGC AGCGAAGCGC TTGTGTCGCC GCTACTCGCA GAAGCTGATC
    CAGCACACCA GCTGCCAGCT GCTGCGCACG TACCCAGCGG CCACACGCAT TGACTCTGCC
    AACCCCAATC CGCTCATCTT CTGGCTGCAC GGAGTCCAGC TGGTGGCCCT CAACTACCAG
    ACTGATGATC TGCCGATGCA GCTGAACGCG GCTCTATTCG AGGCTAATGG TCACTGTGGT
    TTTGTGCTGA AGCCTCCAGT CCTGTGGGAC AGAAGCTGTC CTCTCTATCA GCACTTTTAC
    CCTCTGGACC GAGACCTGGA GAACATGACC CCAACGCTAT ACACACTCAC TATTGTGTCG
    GGGCAGAACG TGTGTCCAGG AAACTCAAAC GGCAGTCCGT GTGTCGAAGT GGAGGTGTTG
    GGAATGCCGG CGGACAGCTG TCATTTCCGA ACTAAACCCA TACACCGCAA CACACTCAAT
    CCCATGTGGA ACGAACACTT CCAGTTCCAC GTGCACTTTG AAGACCTGGC GTTCCTGCGC
    ATCGCTGTGG TGGAGAACAA CAGCTCTCAG GTCACCGCTC AGAGGATACT TCCCCTCAAA
    ACACTCAGAG CAGGCTACCG GCACCTGCAG CTGAGGAACC TGCACAACGA GCCTCTGGAG
    GTGTCCAGTC TGTTCATGTT CAGCCGCAGG ACGGAGGAGA GCCCGACCGG AGGACCACCG
    TCTGCGTCTG TGCTGTTCAG CACCGAGGAG AGACGATCTG TGCAGCAGCA CAAGGTGACC
    GTGCATGGAG TCCCCGGACC TGAACCCTTC ACTGTGATCT GTGTCGATGA ATTCACCACA
    GCCAAACAGC TCCTGGACTC TCTGTTTCCG ACTGCCTCTT TTAAGTATAT GCTGATGGAG
    GAGAGAGTGT CTCTGTCGAA GGAGAAGAAG GCTCCTCAGC AGCGTCCTCT AGCCAATGAT
    GAGAGACTGC TGAAGCTCAT TCACAGCTGG CAGCCTGAGG ACGGATATGT GGGCAGAATA
    TACCTCAAAA CCAGAGAACA GCAGAACTGC AGTGAGAAGA CGAGCGTCCC GCTGGAGTCA
    CTGGAGGAGC TCGGCAGCTT AGAGGACGAC ACCTTCTTCG TGCAGGTCCA TGATGTTTCT
    CCAGAACAGC CTCACACCGT CATCAAAGCC CCGCGATACA GCACAGCACA GGACATCATC
    CAGCAGACTC TGAGCAAAGC CAAGTATTCT CTGAGTATCC TGAGCAACCC CAACCCATGT
    GACTACGTCC TGATGGAGGA GCTTTGTAAA GACGCGGGTG GAAAGAAGTC GTCCTCTGCC
    AAACCGTGCC AGCGTGTGCT GCAGGACCAC GAGTGTGTGT TTCAGGCTCA GAGCCGCTGG
    AAGGGGGCTG GAAAATTCAT CCTCAAACTC AAAGAGCAGT TGGCTCGTGA GGACAAGCGT
    AAAGGTGTGT CGTTCGCCAG TGAGCTGAGG AAGCTGACGG GTCGCAGTCG CAGTGTGACG
    GTCAACACAA ACAGCAACGA CACTCACAGT AAGGAAGAGA AGAGCGCATG TCCCAGCATG
    AGCTGTGTGC CGGAGACATC GCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335827.1
    CDS432..7178
    Translation

    Target ORF information:

    RefSeq Version XM_021480152.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480152.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GAAAGGACCT
    GGGACTGAAC GAGTCCTGTT TTCAATCGGC GACCTCTCAG ACTCTGAAGG GGACCCACTT
    GAGACAGTTA AGGATGTTGT AGACCTCCAG ACCACCGAAG ATGTTCGCGG ACCCTTTAGC
    CATGGGACTG AGCTCATTCC CTGGTACGTT CTGTCGCTTC AGCCGGATAT CCATCAGTTC
    CTGCTCCAAG GAGCAACCGT CATCCACTAC GACCCCGAGA GCCACCTCAC CGCCCGCTGT
    CTTCTCCGTC TACAGCCCGA TAACTGCTTC CTCACCTGGT GCAAACCCCA CAGTAGCTGC
    AGCCTATATG GAAGAGCTAG AACCTTCATG GGACATCCAG CATCCCCAGA CCACTTACAC
    ATCGGCCAAC CTGTTCACTG CGGACTCTCT GACGGGCTCC TGGATCTTAA TGTGGTGAAG
    GCTGTGTTTA TGGGTCACCC TGGAGTGGAC GTCAACTATG TCTGCCTGCA GCACAAACTG
    TGTAACATGA ATCCTGGTGA AAATGGTGTC ACGCTGCTAT ATGGACTTCA CACCACAGAC
    AACAGGCTCC TGCACTTTGT GGCCCCGAAA CACACGGCTC GCATGCTGCA CGAGGGCCTC
    CAGGAGCTGC TGAACTCTAT TAGGAAGATC AGGAAATTCC CTGACCAGAG ACTGCAATGG
    CTGAGAAAGC AATATGTTAG TTTGTACCAG GAGGATGGTA GATTTGAAGG ACCTACACTG
    GCTCATGCTA TCGAGCTGTT CGGAGGGAGA CGCTGGAATA TGGGAACTAG CGGCCCTGGA
    TCCGCCAGCA GAGGTGCAGA GAAGAACTCG GCTCAGAAGA ACAGTCCACT GGGCATCAAC
    TCCAATGTCA AAAAAAAGAA AAAGGCCTTA GTCAGGGGGG ACAGTGGCGA TGGAACTGAT
    GATGAGATGA CCGCACGCAA GACCAGGAGT TGCAAAGAGA CTTTGGGGAG GAGAGAGTCA
    GATGCTCTGG AAAATGTTGA GCAGGAAGAG GCTGAGGACT GCAGTACCTT TAGCCAACCT
    GGACCATTTT CCTTATCCAA CAACAAAGGC CAGTCTCCGG GATCCTCCTC GTCCTCCTCC
    ACCTCTTCTT CTGTTGCCTC CTTTTGCACA AACTCCTCCA CCCCTACACG TCCACATTCC
    TCCCCAATCC TCCCCACAAA CCTCAAGAGT CAGCCAGGGG CCTGGAGCAG TCGAAGCTGG
    CACGGTCGAG GGAAAGGCTG CTTCAGAGGT TTCCAGGACC TCATGATCTC CGACAGCATC
    ATGAGCTTTG TCGAATTTGT GGAGCTCTTC AAATCTTTTA GCATTCGCAG TCGTAAGGAT
    CTGAAGGAAC TATTCGACAC CTACGCCGTG CCCTGCAGTC GCTCTGGCCC TGAGTCTGTC
    CCCCTCTACA CCACACTTAG GATTGACGAC AAGCTAACCG GACTGCAGCC AGATCTGGAT
    CTCCTCACTC GCAACGGCTC TGACCTGGGC CTTTTCATTC GCACCCGCCA GCAAATGTCT
    GAAAACCAGA AGCAGATTTC AGACGCCATC GCAGCTGCCA GTATTGTGAC CAATGGCACT
    GGAGTGGAGA ATTCATCGCT GGGTGTGCTG GGACTGGCCA TCTCGCAGCT CAATGATTTC
    TTGGTCAACT GTCAGAGAGA ACATCTGAGC TATGATGAGA TCCTCAGCAT CATACAGAAA
    TTTGAGCCTT CTTCAAACAT GCGTCAGATG GGGTGGATGT CGTTTGAAGG ATTCGCCAGG
    TTTCTAATGG ACAAGGACAA CTTTGCCTCC AAAAATGAGG AGTCTCAAGT GAACCTGGAT
    GAGCTCCAGC ATCCGCTGTC TTACTATTAC ATCGAGTCCT CTCATAACAC ATACCTGACG
    GGGCATCAGC TGAAGGGAGA GTCTTCAGTC GAGCTCTACA GCCAGGTGTT GCTGCAGGGT
    TGTCGCAGCG TTGAGTTGGA CTGCTGGGAT GGGGATGACG GCATGCCTGT TATTTACCAT
    GGACATACTC TTACCACTAA GATTCCTTTC AAGGATGTTG TAGAGGCCAT CAACCGTGCA
    GCATTTGTGA ACTCAGAGAT GCCTGTGATT CTGTCCATTG AAAACCACTG CTCCTTACCA
    CAGCAGCGCA AAATGGCTGA GATTTTTAAG ATGGTGTTTG GTGAGAAGCT GGTGACCAAG
    TTTCTGTTTG AAAGTGATTT CGCGGACGAG CCTTTGCTTC CGTCTCCCCT TCAGCTCCGG
    GGGAAAATCC TCCTGAAGAA TAAAAAGTTA AAAGCTCACC AGGCTCCTGT TGACATTCTC
    AAACAAAAGG CGCATCAGCT TGCCCACATG CAGGCTCAGG CCAACAACGG CACAGTCAGC
    ACAACGCCAC TGGGAAACAA TGATGAAGAG GAGGAGGAGG AAGACGAGTA CGATTACGAT
    TACGAGTCGC TGTCTGACGA CAATATTCTG GATGACAAAC CTGAAGGCAA GTCATCTACG
    GAGAAACTTC AGTACGAGTC CAACGATGAG ATGCCAAAGC GGTTTAAAAA AGCTGGAAGC
    AAAGGAAAGA TGTTTGACAT GGAGCTGGGA GAGGAGTTTT ACCTGCCCCA GAACGAGAAG
    GAGAGTAGAC AGATTGCTCA AGAGCTGTCA GATCTGGTCA TCTACTGCCA GGCTATCAAA
    TTTCCAGGCC TCTCCACCCT CTCTCCATCA GGCTCTGGAA GGGGGAAAGA CAGAAAGAGC
    CGGAAATCCA TCTTTGGCAG CGCTCCTGCT CGCGGGAGCC CCGGGGAGCC TGTGACGCTG
    GTCCGAGCTC CAGGGAAAGC CAGTCTTGAG GGCATTCGCA TGAACTCGGA GGATCAATTG
    TGTCTGAGCC CGAGCACCTC TCTGAGCTCC ATCATCCGCA CGCCCAAATG CTACCACATC
    TCCTCTGTGA ACGAGAATGC AGCGAAGCGC TTGTGTCGCC GCTACTCGCA GAAGCTGATC
    CAGCACACCA GCTGCCAGCT GCTGCGCACG TACCCAGCGG CCACACGCAT TGACTCTGCC
    AACCCCAATC CGCTCATCTT CTGGCTGCAC GGAGTCCAGC TGGTGGCCCT CAACTACCAG
    ACTGATGATC TGCCGATGCA GCTGAACGCG GCTCTATTCG AGGCTAATGG TCACTGTGGT
    TTTGTGCTGA AGCCTCCAGT CCTGTGGGAC AGAAGCTGTC CTCTCTATCA GCACTTTTAC
    CCTCTGGACC GAGACCTGGA GAACATGACC CCAACGCTAT ACACACTCAC TATTGTGTCG
    GGGCAGAACG TGTGTCCAGG AAACTCAAAC GGCAGTCCGT GTGTCGAAGT GGAGGTGTTG
    GGAATGCCGG CGGACAGCTG TCATTTCCGA ACTAAACCCA TACACCGCAA CACACTCAAT
    CCCATGTGGA ACGAACACTT CCAGTTCCAC GTGCACTTTG AAGACCTGGC GTTCCTGCGC
    ATCGCTGTGG TGGAGAACAA CAGCTCTCAG GTCACCGCTC AGAGGATACT TCCCCTCAAA
    ACACTCAGAG CAGGCTACCG GCACCTGCAG CTGAGGAACC TGCACAACGA GCCTCTGGAG
    GTGTCCAGTC TGTTCATGTT CAGCCGCAGG ACGGAGGAGA GCCCGACCGG AGGACCACCG
    TCTGCGTCTG TGCTGTTCAG CACCGAGGAG AGACGATCTG TGCAGCAGCA CAAGGTGACC
    GTGCATGGAG TCCCCGGACC TGAACCCTTC ACTGTGATCT GTGTCGATGA ATTCACCACA
    GCCAAACAGC TCCTGGACTC TCTGTTTCCG ACTGCCTCTT TTAAGTATAT GCTGATGGAG
    GAGAGAGTGT CTCTGTCGAA GGAGAAGAAG GCTCCTCAGC AGCGTCCTCT AGCCAATGAT
    GAGAGACTGC TGAAGCTCAT TCACAGCTGG CAGCCTGAGG ACGGATATGT GGGCAGAATA
    TACCTCAAAA CCAGAGAACA GCAGAACTGC AGTGAGAAGA CGAGCGTCCC GCTGGAGTCA
    CTGGAGGAGC TCGGCAGCTT AGAGGACGAC ACCTTCTTCG TGCAGGTCCA TGATGTTTCT
    CCAGAACAGC CTCACACCGT CATCAAAGCC CCGCGATACA GCACAGCACA GGACATCATC
    CAGCAGACTC TGAGCAAAGC CAAGTATTCT CTGAGTATCC TGAGCAACCC CAACCCATGT
    GACTACGTCC TGATGGAGGA GCTTTGTAAA GACGCGGGTG GAAAGAAGTC GTCCTCTGCC
    AAACCGTGCC AGCGTGTGCT GCAGGACCAC GAGTGTGTGT TTCAGGCTCA GAGCCGCTGG
    AAGGGGGCTG GAAAATTCAT CCTCAAACTC AAAGAGCAGT TGGCTCGTGA GGACAAGCGT
    AAAGGTGTGT CGTTCGCCAG TGAGCTGAGG AAGCTGACGG GTCGCAGTCG CAGTGTGACG
    GTCAACACAA ACAGCAACGA CACTCACAGT AAGGAAGAGA AGAGCGCATG TCCCAGCATG
    AGCTGTGTGC CGGAGACATC GCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148227
    Clone ID Related Accession (Same CDS sequence) XM_021480154.1
    Accession Version XM_021480154.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GTTTTCAATC
    GGCGACCTCT CAGACTCTGA AGGGGACCCA CTTGAGACAG TTAAGGATGT TGTAGACCTC
    CAGACCACCG AAGATGTTCG CGGACCCTTT AGCCATGGGA CTGAGCTCAT TCCCTGGTAC
    GTTCTGTCGC TTCAGCCGGA TATCCATCAG TTCCTGCTCC AAGGAGCAAC CGTCATCCAC
    TACGACCCCG AGAGCCACCT CACCGCCCGC TGTCTTCTCC GTCTACAGCC CGATAACTGC
    TTCCTCACCT GGTGCAAACC CCACAGTAGC TGCAGCCTAT ATGGAAGAGC TAGAACCTTC
    ATGGGACATC CAGCATCCCC AGACCACTTA CACATCGGCC AACCTGTTCA CTGCGGACTC
    TCTGACGGGC TCCTGGATCT TAATGTGGTG AAGGCTGTGT TTATGGGTCA CCCTGGAGTG
    GACGTCAACT ATGTCTGCCT GCAGCACAAA CTGTGTAACA TGAATCCTGG TGAAAATGGT
    GTCACGCTGC TATATGGACT TCACACCACA GACAACAGGC TCCTGCACTT TGTGGCCCCG
    AAACACACGG CTCGCATGCT GCACGAGGGC CTCCAGGAGC TGCTGAACTC TATTAGGAAG
    ATCAGGAAAT TCCCTGACCA GAGACTGCAA TGGCTGAGAA AGCAATATGT TAGTTTGTAC
    CAGGAGGATG GTAGATTTGA AGGACCTACA CTGGCTCATG CTATCGAGCT GTTCGGAGGG
    AGACGCTGGA ATATGGGAAC TAGCGGCCCT GGATCCGCCA GCAGAGGTGC AGAGAAGAAC
    TCGGCTCAGA AGAACAGTCC ACTGGGCATC AACTCCAATG TCAAAAAAAA GAAAAAGGCC
    TTAGTCAGGG GGGACAGTGG CGATGGAACT GATGATGAGA TGACCGCACG CAAGACCAGG
    AGTTGCAAAG AGACTTTGGG GAGGAGAGAG TCAGATGCTC TGGAAAATGT TGAGCAGGAA
    GAGGCTGAGG ACTGCAGTAC CTTTAGCCAA CCTGGACCAT TTTCCTTATC CAACAACAAA
    GGCCAGTCTC CGGGATCCTC CTCGTCCTCC TCCACCTCTT CTTCTGTTGC CTCCTTTTGC
    ACAAACTCCT CCACCCCTAC ACGTCCACAT TCCTCCCCAA TCCTCCCCAC AAACCTCAAG
    AGTCAGCCAG GGGCCTGGAG CAGTCGAAGC TGGCACGGTC GAGGGAAAGG CTGCTTCAGA
    GGTTTCCAGG ACCTCATGAT CTCCGACAGC ATCATGAGCT TTGTCGAATT TGTGGAGCTC
    TTCAAATCTT TTAGCATTCG CAGTCGTAAG GATCTGAAGG AACTATTCGA CACCTACGCC
    GTGCCCTGCA GTCGCTCTGG CCCTGAGTCT GTCCCCCTCT ACACCACACT TAGGATTGAC
    GACAAGCTAA CCGGACTGCA GCCAGATCTG GATCTCCTCA CTCGCAACGG CTCTGACCTG
    GGCCTTTTCA TTCGCACCCG CCAGCAAATG TCTGAAAACC AGAAGCAGAT TTCAGACGCC
    ATCGCAGCTG CCAGTATTGT GACCAATGGC ACTGGAGTGG AGAATTCATC GCTGGGTGTG
    CTGGGACTGG CCATCTCGCA GCTCAATGAT TTCTTGGTCA ACTGTCAGAG AGAACATCTG
    AGCTATGATG AGATCCTCAG CATCATACAG AAATTTGAGC CTTCTTCAAA CATGCGTCAG
    ATGGGGTGGA TGTCGTTTGA AGGATTCGCC AGGTTTCTAA TGGACAAGGA CAACTTTGCC
    TCCAAAAATG AGGAGTCTCA AGTGAACCTG GATGAGCTCC AGCATCCGCT GTCTTACTAT
    TACATCGAGT CCTCTCATAA CACATACCTG ACGGGGCATC AGCTGAAGGG AGAGTCTTCA
    GTCGAGCTCT ACAGCCAGGT GTTGCTGCAG GGTTGTCGCA GCGTTGAGTT GGACTGCTGG
    GATGGGGATG ACGGCATGCC TGTTATTTAC CATGGACATA CTCTTACCAC TAAGATTCCT
    TTCAAGGATG TTGTAGAGGC CATCAACCGT GCAGCATTTG TGAACTCAGA GATGCCTGTG
    ATTCTGTCCA TTGAAAACCA CTGCTCCTTA CCACAGCAGC GCAAAATGGC TGAGATTTTT
    AAGATGGTGT TTGGTGAGAA GCTGGTGACC AAGTTTCTGT TTGAAAGTGA TTTCGCGGAC
    GAGCCTTTGC TTCCGTCTCC CCTTCAGCTC CGGGGGAAAA TCCTCCTGAA GAATAAAAAG
    TTAAAAGCTC ACCAGGCTCC TGTTGACATT CTCAAACAAA AGGCGCATCA GCTTGCCCAC
    ATGCAGGCTC AGGCCAACAA CGGCACAGTC AGCACAACGC CACTGGGAAA CAATGATGAA
    GAGGAGGAGG AGGAAGACGA GTACGATTAC GATTACGAGT CGCTGTCTGA CGACAATATT
    CTGGATGACA AACCTGAAGG CAAGTCATCT ACGGAGAAAC TTCAGTACGA GTCCAACGAT
    GAGATGCCAA AGCGGTTTAA AAAAGCTGGA AGCAAAGGAA AGATGTTTGA CATGGAGCTG
    GGAGAGGAGT TTTACCTGCC CCAGAACGAG AAGGAGAGTA GACAGATTGC TCAAGAGCTG
    TCAGATCTGG TCATCTACTG CCAGGCTATC AAATTTCCAG GCCTCTCCAC CCTCTCTCCA
    TCAGGCTCTG GAAGGGGGAA AGACAGAAAG AGCCGGAAAT CCATCTTTGG CAGCGCTCCT
    GCTCGCGGGA GCCCCGGGGA GCCTGTGACG CTGGTCCGAG CTCCAGGGAA AGCCAGTCTT
    GAGGGCATTC GCATGAACTC GGAGGATCAA TTGTGTCTGA GCCCGAGCAC CTCTCTGAGC
    TCCATCATCC GCACGCCCAA ATGCTACCAC ATCTCCTCTG TGAACGAGAA TGCAGCGAAG
    CGCTTGTGTC GCCGCTACTC GCAGAAGCTG ATCCAGCACA CCAGCTGCCA GCTGCTGCGC
    ACGTACCCAG CGGCCACACG CATTGACTCT GCCAACCCCA ATCCGCTCAT CTTCTGGCTG
    CACGGAGTCC AGCTGGTGGC CCTCAACTAC CAGACTGATG ATCTGCCGAT GCAGCTGAAC
    GCGGCTCTAT TCGAGGCTAA TGGTCACTGT GGTTTTGTGC TGAAGCCTCC AGTCCTGTGG
    GACAGAAGCT GTCCTCTCTA TCAGCACTTT TACCCTCTGG ACCGAGACCT GGAGAACATG
    ACCCCAACGC TATACACACT CACTATTGTG TCGGGGCAGA ACGTGTGTCC AGGAAACTCA
    AACGGCAGTC CGTGTGTCGA AGTGGAGGTG TTGGGAATGC CGGCGGACAG CTGTCATTTC
    CGAACTAAAC CCATACACCG CAACACACTC AATCCCATGT GGAACGAACA CTTCCAGTTC
    CACGTGCACT TTGAAGACCT GGCGTTCCTG CGCATCGCTG TGGTGGAGAA CAACAGCTCT
    CAGGTCACCG CTCAGAGGAT ACTTCCCCTC AAAACACTCA GAGCAGGCTA CCGGCACCTG
    CAGCTGAGGA ACCTGCACAA CGAGCCTCTG GAGGTGTCCA GTCTGTTCAT GTTCAGCCGC
    AGGACGGAGG AGAGCCCGAC CGGAGGACCA CCGTCTGCGT CTGTGCTGTT CAGCACCGAG
    GAGAGACGAT CTGTGCAGCA GCACAAGGTG ACCGTGCATG GAGTCCCCGG ACCTGAACCC
    TTCACTGTGA TCTGTGTCGA TGAATTCACC ACAGCCAAAC AGCTCCTGGA CTCTCTGTTT
    CCGACTGCCT CTTTTAAGTA TATGCTGATG GAGGAGAGAG TGTCTCTGTC GAAGGAGAAG
    AAGGCTCCTC AGCAGCGTCC TCTAGCCAAT GATGAGAGAC TGCTGAAGCT CATTCACAGC
    TGGCAGCCTG AGGACGGATA TGTGGGCAGA ATATACCTCA AAACCAGAGA ACAGAACTGC
    AGTGAGAAGA CGAGCGTCCC GCTGGAGTCA CTGGAGGAGC TCGGCAGCTT AGAGGACGAC
    ACCTTCTTCG TGCAGGTCCA TGATGTTTCT CCAGAACAGC CTCACACCGT CATCAAAGCC
    CCGCGATACA GCACAGCACA GGACATCATC CAGCAGACTC TGAGCAAAGC CAAGTATTCT
    CTGAGTATCC TGAGCAACCC CAACCCATGT GACTACGTCC TGATGGAGGA GCTTTGTAAA
    GACGCGGGTG GAAAGAAGTC GTCCTCTGCC AAACCGTGCC AGCGTGTGCT GCAGGACCAC
    GAGTGTGTGT TTCAGGCTCA GAGCCGCTGG AAGGGGGCTG GAAAATTCAT CCTCAAACTC
    AAAGAGCAGT TGGCTCGTGA GGACAAGCGT AAAGGTGTGT CGTTCGCCAG TGAGCTGAGG
    AAGCTGACGG GTCGCAGTCG CAGTGTGACG GTCAACACAA ACAGCAACGA CACTCACAGG
    TCTCTGTCTC TGGGGCTGAA GGCCTGGATG GCTGAGGGCC GCTTGGGCTC TCCGAGACGC
    TCCCAGTGTG GTGACGGTGT CTCGGCCTTG TCCGGGTGGT CTCTCCCGCT GCTGTGGAAC
    TGGAAGCTGC ATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335829.1
    CDS432..7229
    Translation

    Target ORF information:

    RefSeq Version XM_021480154.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480154.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GTTTTCAATC
    GGCGACCTCT CAGACTCTGA AGGGGACCCA CTTGAGACAG TTAAGGATGT TGTAGACCTC
    CAGACCACCG AAGATGTTCG CGGACCCTTT AGCCATGGGA CTGAGCTCAT TCCCTGGTAC
    GTTCTGTCGC TTCAGCCGGA TATCCATCAG TTCCTGCTCC AAGGAGCAAC CGTCATCCAC
    TACGACCCCG AGAGCCACCT CACCGCCCGC TGTCTTCTCC GTCTACAGCC CGATAACTGC
    TTCCTCACCT GGTGCAAACC CCACAGTAGC TGCAGCCTAT ATGGAAGAGC TAGAACCTTC
    ATGGGACATC CAGCATCCCC AGACCACTTA CACATCGGCC AACCTGTTCA CTGCGGACTC
    TCTGACGGGC TCCTGGATCT TAATGTGGTG AAGGCTGTGT TTATGGGTCA CCCTGGAGTG
    GACGTCAACT ATGTCTGCCT GCAGCACAAA CTGTGTAACA TGAATCCTGG TGAAAATGGT
    GTCACGCTGC TATATGGACT TCACACCACA GACAACAGGC TCCTGCACTT TGTGGCCCCG
    AAACACACGG CTCGCATGCT GCACGAGGGC CTCCAGGAGC TGCTGAACTC TATTAGGAAG
    ATCAGGAAAT TCCCTGACCA GAGACTGCAA TGGCTGAGAA AGCAATATGT TAGTTTGTAC
    CAGGAGGATG GTAGATTTGA AGGACCTACA CTGGCTCATG CTATCGAGCT GTTCGGAGGG
    AGACGCTGGA ATATGGGAAC TAGCGGCCCT GGATCCGCCA GCAGAGGTGC AGAGAAGAAC
    TCGGCTCAGA AGAACAGTCC ACTGGGCATC AACTCCAATG TCAAAAAAAA GAAAAAGGCC
    TTAGTCAGGG GGGACAGTGG CGATGGAACT GATGATGAGA TGACCGCACG CAAGACCAGG
    AGTTGCAAAG AGACTTTGGG GAGGAGAGAG TCAGATGCTC TGGAAAATGT TGAGCAGGAA
    GAGGCTGAGG ACTGCAGTAC CTTTAGCCAA CCTGGACCAT TTTCCTTATC CAACAACAAA
    GGCCAGTCTC CGGGATCCTC CTCGTCCTCC TCCACCTCTT CTTCTGTTGC CTCCTTTTGC
    ACAAACTCCT CCACCCCTAC ACGTCCACAT TCCTCCCCAA TCCTCCCCAC AAACCTCAAG
    AGTCAGCCAG GGGCCTGGAG CAGTCGAAGC TGGCACGGTC GAGGGAAAGG CTGCTTCAGA
    GGTTTCCAGG ACCTCATGAT CTCCGACAGC ATCATGAGCT TTGTCGAATT TGTGGAGCTC
    TTCAAATCTT TTAGCATTCG CAGTCGTAAG GATCTGAAGG AACTATTCGA CACCTACGCC
    GTGCCCTGCA GTCGCTCTGG CCCTGAGTCT GTCCCCCTCT ACACCACACT TAGGATTGAC
    GACAAGCTAA CCGGACTGCA GCCAGATCTG GATCTCCTCA CTCGCAACGG CTCTGACCTG
    GGCCTTTTCA TTCGCACCCG CCAGCAAATG TCTGAAAACC AGAAGCAGAT TTCAGACGCC
    ATCGCAGCTG CCAGTATTGT GACCAATGGC ACTGGAGTGG AGAATTCATC GCTGGGTGTG
    CTGGGACTGG CCATCTCGCA GCTCAATGAT TTCTTGGTCA ACTGTCAGAG AGAACATCTG
    AGCTATGATG AGATCCTCAG CATCATACAG AAATTTGAGC CTTCTTCAAA CATGCGTCAG
    ATGGGGTGGA TGTCGTTTGA AGGATTCGCC AGGTTTCTAA TGGACAAGGA CAACTTTGCC
    TCCAAAAATG AGGAGTCTCA AGTGAACCTG GATGAGCTCC AGCATCCGCT GTCTTACTAT
    TACATCGAGT CCTCTCATAA CACATACCTG ACGGGGCATC AGCTGAAGGG AGAGTCTTCA
    GTCGAGCTCT ACAGCCAGGT GTTGCTGCAG GGTTGTCGCA GCGTTGAGTT GGACTGCTGG
    GATGGGGATG ACGGCATGCC TGTTATTTAC CATGGACATA CTCTTACCAC TAAGATTCCT
    TTCAAGGATG TTGTAGAGGC CATCAACCGT GCAGCATTTG TGAACTCAGA GATGCCTGTG
    ATTCTGTCCA TTGAAAACCA CTGCTCCTTA CCACAGCAGC GCAAAATGGC TGAGATTTTT
    AAGATGGTGT TTGGTGAGAA GCTGGTGACC AAGTTTCTGT TTGAAAGTGA TTTCGCGGAC
    GAGCCTTTGC TTCCGTCTCC CCTTCAGCTC CGGGGGAAAA TCCTCCTGAA GAATAAAAAG
    TTAAAAGCTC ACCAGGCTCC TGTTGACATT CTCAAACAAA AGGCGCATCA GCTTGCCCAC
    ATGCAGGCTC AGGCCAACAA CGGCACAGTC AGCACAACGC CACTGGGAAA CAATGATGAA
    GAGGAGGAGG AGGAAGACGA GTACGATTAC GATTACGAGT CGCTGTCTGA CGACAATATT
    CTGGATGACA AACCTGAAGG CAAGTCATCT ACGGAGAAAC TTCAGTACGA GTCCAACGAT
    GAGATGCCAA AGCGGTTTAA AAAAGCTGGA AGCAAAGGAA AGATGTTTGA CATGGAGCTG
    GGAGAGGAGT TTTACCTGCC CCAGAACGAG AAGGAGAGTA GACAGATTGC TCAAGAGCTG
    TCAGATCTGG TCATCTACTG CCAGGCTATC AAATTTCCAG GCCTCTCCAC CCTCTCTCCA
    TCAGGCTCTG GAAGGGGGAA AGACAGAAAG AGCCGGAAAT CCATCTTTGG CAGCGCTCCT
    GCTCGCGGGA GCCCCGGGGA GCCTGTGACG CTGGTCCGAG CTCCAGGGAA AGCCAGTCTT
    GAGGGCATTC GCATGAACTC GGAGGATCAA TTGTGTCTGA GCCCGAGCAC CTCTCTGAGC
    TCCATCATCC GCACGCCCAA ATGCTACCAC ATCTCCTCTG TGAACGAGAA TGCAGCGAAG
    CGCTTGTGTC GCCGCTACTC GCAGAAGCTG ATCCAGCACA CCAGCTGCCA GCTGCTGCGC
    ACGTACCCAG CGGCCACACG CATTGACTCT GCCAACCCCA ATCCGCTCAT CTTCTGGCTG
    CACGGAGTCC AGCTGGTGGC CCTCAACTAC CAGACTGATG ATCTGCCGAT GCAGCTGAAC
    GCGGCTCTAT TCGAGGCTAA TGGTCACTGT GGTTTTGTGC TGAAGCCTCC AGTCCTGTGG
    GACAGAAGCT GTCCTCTCTA TCAGCACTTT TACCCTCTGG ACCGAGACCT GGAGAACATG
    ACCCCAACGC TATACACACT CACTATTGTG TCGGGGCAGA ACGTGTGTCC AGGAAACTCA
    AACGGCAGTC CGTGTGTCGA AGTGGAGGTG TTGGGAATGC CGGCGGACAG CTGTCATTTC
    CGAACTAAAC CCATACACCG CAACACACTC AATCCCATGT GGAACGAACA CTTCCAGTTC
    CACGTGCACT TTGAAGACCT GGCGTTCCTG CGCATCGCTG TGGTGGAGAA CAACAGCTCT
    CAGGTCACCG CTCAGAGGAT ACTTCCCCTC AAAACACTCA GAGCAGGCTA CCGGCACCTG
    CAGCTGAGGA ACCTGCACAA CGAGCCTCTG GAGGTGTCCA GTCTGTTCAT GTTCAGCCGC
    AGGACGGAGG AGAGCCCGAC CGGAGGACCA CCGTCTGCGT CTGTGCTGTT CAGCACCGAG
    GAGAGACGAT CTGTGCAGCA GCACAAGGTG ACCGTGCATG GAGTCCCCGG ACCTGAACCC
    TTCACTGTGA TCTGTGTCGA TGAATTCACC ACAGCCAAAC AGCTCCTGGA CTCTCTGTTT
    CCGACTGCCT CTTTTAAGTA TATGCTGATG GAGGAGAGAG TGTCTCTGTC GAAGGAGAAG
    AAGGCTCCTC AGCAGCGTCC TCTAGCCAAT GATGAGAGAC TGCTGAAGCT CATTCACAGC
    TGGCAGCCTG AGGACGGATA TGTGGGCAGA ATATACCTCA AAACCAGAGA ACAGAACTGC
    AGTGAGAAGA CGAGCGTCCC GCTGGAGTCA CTGGAGGAGC TCGGCAGCTT AGAGGACGAC
    ACCTTCTTCG TGCAGGTCCA TGATGTTTCT CCAGAACAGC CTCACACCGT CATCAAAGCC
    CCGCGATACA GCACAGCACA GGACATCATC CAGCAGACTC TGAGCAAAGC CAAGTATTCT
    CTGAGTATCC TGAGCAACCC CAACCCATGT GACTACGTCC TGATGGAGGA GCTTTGTAAA
    GACGCGGGTG GAAAGAAGTC GTCCTCTGCC AAACCGTGCC AGCGTGTGCT GCAGGACCAC
    GAGTGTGTGT TTCAGGCTCA GAGCCGCTGG AAGGGGGCTG GAAAATTCAT CCTCAAACTC
    AAAGAGCAGT TGGCTCGTGA GGACAAGCGT AAAGGTGTGT CGTTCGCCAG TGAGCTGAGG
    AAGCTGACGG GTCGCAGTCG CAGTGTGACG GTCAACACAA ACAGCAACGA CACTCACAGG
    TCTCTGTCTC TGGGGCTGAA GGCCTGGATG GCTGAGGGCC GCTTGGGCTC TCCGAGACGC
    TCCCAGTGTG GTGACGGTGT CTCGGCCTTG TCCGGGTGGT CTCTCCCGCT GCTGTGGAAC
    TGGAAGCTGC ATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45393
    Clone ID Related Accession (Same CDS sequence) XM_009306422.2
    Accession Version XM_009306422.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6762bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GTTTTCAATC
    GGCGACCTCT CAGACTCTGA AGGGGACCCA CTTGAGACAG TTAAGGATGT TGTAGACCTC
    CAGACCACCG AAGATGTTCG CGGACCCTTT AGCCATGGGA CTGAGCTCAT TCCCTGGTAC
    GTTCTGTCGC TTCAGCCGGA TATCCATCAG TTCCTGCTCC AAGGAGCAAC CGTCATCCAC
    TACGACCCCG AGAGCCACCT CACCGCCCGC TGTCTTCTCC GTCTACAGCC CGATAACTGC
    TTCCTCACCT GGTGCAAACC CCACAGTAGC TGCAGCCTAT ATGGAAGAGC TAGAACCTTC
    ATGGGACATC CAGCATCCCC AGACCACTTA CACATCGGCC AACCTGTTCA CTGCGGACTC
    TCTGACGGGC TCCTGGATCT TAATGTGGTG AAGGCTGTGT TTATGGGTCA CCCTGGAGTG
    GACGTCAACT ATGTCTGCCT GCAGCACAAA CTGTGTAACA TGAATCCTGG TGAAAATGGT
    GTCACGCTGC TATATGGACT TCACACCACA GACAACAGGC TCCTGCACTT TGTGGCCCCG
    AAACACACGG CTCGCATGCT GCACGAGGGC CTCCAGGAGC TGCTGAACTC TATTAGGAAG
    ATCAGGAAAT TCCCTGACCA GAGACTGCAA TGGCTGAGAA AGCAATATGT TAGTTTGTAC
    CAGGAGGATG GTAGATTTGA AGGACCTACA CTGGCTCATG CTATCGAGCT GTTCGGAGGG
    AGACGCTGGA ATATGGGAAC TAGCGGCCCT GGATCCGCCA GCAGAGGTGC AGAGAAGAAC
    TCGGCTCAGA AGAACAGTCC ACTGGGCATC AACTCCAATG TCAAAAAAAA GAAAAAGGCC
    TTAGTCAGGG GGGACAGTGG CGATGGAACT GATGATGAGA TGACCGCACG CAAGACCAGG
    AGTTGCAAAG AGACTTTGGG GAGGAGAGAG TCAGATGCTC TGGAAAATGT TGAGCAGGAA
    GAGGCTGAGG ACTGCAGTAC CTTTAGCCAA CCTGGACCAT TTTCCTTATC CAACAACAAA
    GGCCAGTCTC CGGGATCCTC CTCGTCCTCC TCCACCTCTT CTTCTGTTGC CTCCTTTTGC
    ACAAACTCCT CCACCCCTAC ACGTCCACAT TCCTCCCCAA TCCTCCCCAC AAACCTCAAG
    AGTCAGCCAG GGGCCTGGAG CAGTCGAAGC TGGCACGGTC GAGGGAAAGG CTGCTTCAGA
    GGTTTCCAGG ACCTCATGAT CTCCGACAGC ATCATGAGCT TTGTCGAATT TGTGGAGCTC
    TTCAAATCTT TTAGCATTCG CAGTCGTAAG GATCTGAAGG AACTATTCGA CACCTACGCC
    GTGCCCTGCA GTCGCTCTGG CCCTGAGTCT GTCCCCCTCT ACACCACACT TAGGATTGAC
    GACAAGCTAA CCGGACTGCA GCCAGATCTG GATCTCCTCA CTCGCAACGG CTCTGACCTG
    GGCCTTTTCA TTCGCACCCG CCAGCAAATG TCTGAAAACC AGAAGCAGAT TTCAGACGCC
    ATCGCAGCTG CCAGTATTGT GACCAATGGC ACTGGAGTGG AGAATTCATC GCTGGGTGTG
    CTGGGACTGG CCATCTCGCA GCTCAATGAT TTCTTGGTCA ACTGTCAGAG AGAACATCTG
    AGCTATGATG AGATCCTCAG CATCATACAG AAATTTGAGC CTTCTTCAAA CATGCGTCAG
    ATGGGGTGGA TGTCGTTTGA AGGATTCGCC AGGTTTCTAA TGGACAAGGA CAACTTTGCC
    TCCAAAAATG AGGAGTCTCA AGTGAACCTG GATGAGCTCC AGCATCCGCT GTCTTACTAT
    TACATCGAGT CCTCTCATAA CACATACCTG ACGGGGCATC AGCTGAAGGG AGAGTCTTCA
    GTCGAGCTCT ACAGCCAGGT GTTGCTGCAG GGTTGTCGCA GCGTTGAGTT GGACTGCTGG
    GATGGGGATG ACGGCATGCC TGTTATTTAC CATGGACATA CTCTTACCAC TAAGATTCCT
    TTCAAGGATG TTGTAGAGGC CATCAACCGT GCAGCATTTG TGAACTCAGA GATGCCTGTG
    ATTCTGTCCA TTGAAAACCA CTGCTCCTTA CCACAGCAGC GCAAAATGGC TGAGATTTTT
    AAGATGGTGT TTGGTGAGAA GCTGGTGACC AAGTTTCTGT TTGAAAGTGA TTTCGCGGAC
    GAGCCTTTGC TTCCGTCTCC CCTTCAGCTC CGGGGGAAAA TCCTCCTGAA GAATAAAAAG
    TTAAAAGCTC ACCAGGCTCC TGTTGACATT CTCAAACAAA AGGCGCATCA GCTTGCCCAC
    ATGCAGGCTC AGGCCAACAA CGGCACAGTC AGCACAACGC CACTGGGAAA CAATGATGAA
    GAGGAGGAGG AGGAAGACGA GTACGATTAC GATTACGAGT CGCTGTCTGA CGCTGATGTC
    CTCAATGCTT CCACAGCCTC TTATGGCCAG GAAGACAATA TTCTGGATGA CAAACCTGAA
    GGCAAGTCAT CTACGGAGAA ACTTCAGTAC GAGTCCAACG ATGAGATGCC AAAGCGGTTT
    AAAAAAGCTG GAAGCAAAGG AAAGATGTTT GACATGGAGC TGGGAGAGGA GTTTTACCTG
    CCCCAGAACG AGAAGGAGAG TAGACAGATT GCTCAAGAGC TGTCAGATCT GGTCATCTAC
    TGCCAGGCTA TCAAATTTCC AGGCCTCTCC ACCCTCTCTC CATCAGGCTC TGGAAGGGGG
    AAAGACAGAA AGAGCCGGAA ATCCATCTTT GGCAGCGCTC CTGCTCGCGG GAGCCCCGGG
    GAGCCTGTGA CGCTGGTCCG AGCTCCAGGG AAAGCCAGTC TTGAGGGCAT TCGCATGAAC
    TCGGAGGATC AATTGTGTCT GAGCCCGAGC ACCTCTCTGA GCTCCATCAT CCGCACGCCC
    AAATGCTACC ACATCTCCTC TGTGAACGAG AATGCAGCGA AGCGCTTGTG TCGCCGCTAC
    TCGCAGAAGC TGATCCAGCA CACCAGCTGC CAGCTGCTGC GCACGTACCC AGCGGCCACA
    CGCATTGACT CTGCCAACCC CAATCCGCTC ATCTTCTGGC TGCACGGAGT CCAGCTGGTG
    GCCCTCAACT ACCAGACTGA TGATCTGCCG ATGCAGCTGA ACGCGGCTCT ATTCGAGGCT
    AATGGTCACT GTGGTTTTGT GCTGAAGCCT CCAGTCCTGT GGGACAGAAG CTGTCCTCTC
    TATCAGCACT TTTACCCTCT GGACCGAGAC CTGGAGAACA TGACCCCAAC GCTATACACA
    CTCACTATTG TGTCGGGGCA GAACGTGTGT CCAGGAAACT CAAACGGCAG TCCGTGTGTC
    GAAGTGGAGG TGTTGGGAAT GCCGGCGGAC AGCTGTCATT TCCGAACTAA ACCCATACAC
    CGCAACACAC TCAATCCCAT GTGGAACGAA CACTTCCAGT TCCACGTGCA CTTTGAAGAC
    CTGGCGTTCC TGCGCATCGC TGTGGTGGAG AACAACAGCT CTCAGGTCAC CGCTCAGAGG
    ATACTTCCCC TCAAAACACT CAGAGCAGGC TACCGGCACC TGCAGCTGAG GAACCTGCAC
    AACGAGCCTC TGGAGGTGTC CAGTCTGTTC ATGTTCAGCC GCAGGACGGA GGAGAGCCCG
    ACCGGAGGAC CACCGTCTGC GTCTGTGCTG TTCAGCACCG AGGAGAGACG ATCTGTGCAG
    CAGCACAAGG TGACCGTGCA TGGAGTCCCC GGACCTGAAC CCTTCACTGT GATCTGTGTC
    GATGAATTCA CCACAGCCAA ACAGCTCCTG GACTCTCTGT TTCCGACTGC CTCTTTTAAG
    TATATGCTGA TGGAGGAGAG AGTGTCTCTG TCGAAGGAGA AGAAGGCTCC TCAGCAGCGT
    CCTCTAGCCA ATGATGAGAG ACTGCTGAAG CTCATTCACA GCTGGCAGCC TGAGGACGGA
    TATGTGGGCA GAATATACCT CAAAACCAGA GAACAGCAGA ACTGCAGTGA GAAGACGAGC
    GTCCCGCTGG AGTCACTGGA GGAGCTCGGC AGCTTAGAGG ACGACACCTT CTTCGTGCAG
    GTCCATGATG TTTCTCCAGA ACAGCCTCAC ACCGTCATCA AAGCCCCGCG ATACAGCACA
    GCACAGGACA TCATCCAGCA GACTCTGAGC AAAGCCAAGT ATTCTCTGAG TATCCTGAGC
    AACCCCAACC CATGTGACTA CGTCCTGATG GAGGAGCTTT GTAAAGACGC GGGTGGAAAG
    AAGTCGTCCT CTGCCAAACC GTGCCAGCGT GTGCTGCAGG ACCACGAGTG TGTGTTTCAG
    GCTCAGAGCC GCTGGAAGGG GGCTGGAAAA TTCATCCTCA AACTCAAAGA GCAGTTGGCT
    CGTGAGGACA AGCGTAAAGG TGTGTCGTTC GCCAGTGAGC TGAGGAAGCT GACGGGTCGC
    AGTCGCAGTG TGACGGTCAA CACAAACAGC AACGACACTC ACAGTAAGGA AGAGAAGAGC
    GCATGTCCCA GCATGAGCTG TGTGCCGGAG ACATCGCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304697.1
    CDS419..7180
    Translation

    Target ORF information:

    RefSeq Version XM_009306422.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306422.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GTTTTCAATC
    GGCGACCTCT CAGACTCTGA AGGGGACCCA CTTGAGACAG TTAAGGATGT TGTAGACCTC
    CAGACCACCG AAGATGTTCG CGGACCCTTT AGCCATGGGA CTGAGCTCAT TCCCTGGTAC
    GTTCTGTCGC TTCAGCCGGA TATCCATCAG TTCCTGCTCC AAGGAGCAAC CGTCATCCAC
    TACGACCCCG AGAGCCACCT CACCGCCCGC TGTCTTCTCC GTCTACAGCC CGATAACTGC
    TTCCTCACCT GGTGCAAACC CCACAGTAGC TGCAGCCTAT ATGGAAGAGC TAGAACCTTC
    ATGGGACATC CAGCATCCCC AGACCACTTA CACATCGGCC AACCTGTTCA CTGCGGACTC
    TCTGACGGGC TCCTGGATCT TAATGTGGTG AAGGCTGTGT TTATGGGTCA CCCTGGAGTG
    GACGTCAACT ATGTCTGCCT GCAGCACAAA CTGTGTAACA TGAATCCTGG TGAAAATGGT
    GTCACGCTGC TATATGGACT TCACACCACA GACAACAGGC TCCTGCACTT TGTGGCCCCG
    AAACACACGG CTCGCATGCT GCACGAGGGC CTCCAGGAGC TGCTGAACTC TATTAGGAAG
    ATCAGGAAAT TCCCTGACCA GAGACTGCAA TGGCTGAGAA AGCAATATGT TAGTTTGTAC
    CAGGAGGATG GTAGATTTGA AGGACCTACA CTGGCTCATG CTATCGAGCT GTTCGGAGGG
    AGACGCTGGA ATATGGGAAC TAGCGGCCCT GGATCCGCCA GCAGAGGTGC AGAGAAGAAC
    TCGGCTCAGA AGAACAGTCC ACTGGGCATC AACTCCAATG TCAAAAAAAA GAAAAAGGCC
    TTAGTCAGGG GGGACAGTGG CGATGGAACT GATGATGAGA TGACCGCACG CAAGACCAGG
    AGTTGCAAAG AGACTTTGGG GAGGAGAGAG TCAGATGCTC TGGAAAATGT TGAGCAGGAA
    GAGGCTGAGG ACTGCAGTAC CTTTAGCCAA CCTGGACCAT TTTCCTTATC CAACAACAAA
    GGCCAGTCTC CGGGATCCTC CTCGTCCTCC TCCACCTCTT CTTCTGTTGC CTCCTTTTGC
    ACAAACTCCT CCACCCCTAC ACGTCCACAT TCCTCCCCAA TCCTCCCCAC AAACCTCAAG
    AGTCAGCCAG GGGCCTGGAG CAGTCGAAGC TGGCACGGTC GAGGGAAAGG CTGCTTCAGA
    GGTTTCCAGG ACCTCATGAT CTCCGACAGC ATCATGAGCT TTGTCGAATT TGTGGAGCTC
    TTCAAATCTT TTAGCATTCG CAGTCGTAAG GATCTGAAGG AACTATTCGA CACCTACGCC
    GTGCCCTGCA GTCGCTCTGG CCCTGAGTCT GTCCCCCTCT ACACCACACT TAGGATTGAC
    GACAAGCTAA CCGGACTGCA GCCAGATCTG GATCTCCTCA CTCGCAACGG CTCTGACCTG
    GGCCTTTTCA TTCGCACCCG CCAGCAAATG TCTGAAAACC AGAAGCAGAT TTCAGACGCC
    ATCGCAGCTG CCAGTATTGT GACCAATGGC ACTGGAGTGG AGAATTCATC GCTGGGTGTG
    CTGGGACTGG CCATCTCGCA GCTCAATGAT TTCTTGGTCA ACTGTCAGAG AGAACATCTG
    AGCTATGATG AGATCCTCAG CATCATACAG AAATTTGAGC CTTCTTCAAA CATGCGTCAG
    ATGGGGTGGA TGTCGTTTGA AGGATTCGCC AGGTTTCTAA TGGACAAGGA CAACTTTGCC
    TCCAAAAATG AGGAGTCTCA AGTGAACCTG GATGAGCTCC AGCATCCGCT GTCTTACTAT
    TACATCGAGT CCTCTCATAA CACATACCTG ACGGGGCATC AGCTGAAGGG AGAGTCTTCA
    GTCGAGCTCT ACAGCCAGGT GTTGCTGCAG GGTTGTCGCA GCGTTGAGTT GGACTGCTGG
    GATGGGGATG ACGGCATGCC TGTTATTTAC CATGGACATA CTCTTACCAC TAAGATTCCT
    TTCAAGGATG TTGTAGAGGC CATCAACCGT GCAGCATTTG TGAACTCAGA GATGCCTGTG
    ATTCTGTCCA TTGAAAACCA CTGCTCCTTA CCACAGCAGC GCAAAATGGC TGAGATTTTT
    AAGATGGTGT TTGGTGAGAA GCTGGTGACC AAGTTTCTGT TTGAAAGTGA TTTCGCGGAC
    GAGCCTTTGC TTCCGTCTCC CCTTCAGCTC CGGGGGAAAA TCCTCCTGAA GAATAAAAAG
    TTAAAAGCTC ACCAGGCTCC TGTTGACATT CTCAAACAAA AGGCGCATCA GCTTGCCCAC
    ATGCAGGCTC AGGCCAACAA CGGCACAGTC AGCACAACGC CACTGGGAAA CAATGATGAA
    GAGGAGGAGG AGGAAGACGA GTACGATTAC GATTACGAGT CGCTGTCTGA CGCTGATGTC
    CTCAATGCTT CCACAGCCTC TTATGGCCAG GAAGACAATA TTCTGGATGA CAAACCTGAA
    GGCAAGTCAT CTACGGAGAA ACTTCAGTAC GAGTCCAACG ATGAGATGCC AAAGCGGTTT
    AAAAAAGCTG GAAGCAAAGG AAAGATGTTT GACATGGAGC TGGGAGAGGA GTTTTACCTG
    CCCCAGAACG AGAAGGAGAG TAGACAGATT GCTCAAGAGC TGTCAGATCT GGTCATCTAC
    TGCCAGGCTA TCAAATTTCC AGGCCTCTCC ACCCTCTCTC CATCAGGCTC TGGAAGGGGG
    AAAGACAGAA AGAGCCGGAA ATCCATCTTT GGCAGCGCTC CTGCTCGCGG GAGCCCCGGG
    GAGCCTGTGA CGCTGGTCCG AGCTCCAGGG AAAGCCAGTC TTGAGGGCAT TCGCATGAAC
    TCGGAGGATC AATTGTGTCT GAGCCCGAGC ACCTCTCTGA GCTCCATCAT CCGCACGCCC
    AAATGCTACC ACATCTCCTC TGTGAACGAG AATGCAGCGA AGCGCTTGTG TCGCCGCTAC
    TCGCAGAAGC TGATCCAGCA CACCAGCTGC CAGCTGCTGC GCACGTACCC AGCGGCCACA
    CGCATTGACT CTGCCAACCC CAATCCGCTC ATCTTCTGGC TGCACGGAGT CCAGCTGGTG
    GCCCTCAACT ACCAGACTGA TGATCTGCCG ATGCAGCTGA ACGCGGCTCT ATTCGAGGCT
    AATGGTCACT GTGGTTTTGT GCTGAAGCCT CCAGTCCTGT GGGACAGAAG CTGTCCTCTC
    TATCAGCACT TTTACCCTCT GGACCGAGAC CTGGAGAACA TGACCCCAAC GCTATACACA
    CTCACTATTG TGTCGGGGCA GAACGTGTGT CCAGGAAACT CAAACGGCAG TCCGTGTGTC
    GAAGTGGAGG TGTTGGGAAT GCCGGCGGAC AGCTGTCATT TCCGAACTAA ACCCATACAC
    CGCAACACAC TCAATCCCAT GTGGAACGAA CACTTCCAGT TCCACGTGCA CTTTGAAGAC
    CTGGCGTTCC TGCGCATCGC TGTGGTGGAG AACAACAGCT CTCAGGTCAC CGCTCAGAGG
    ATACTTCCCC TCAAAACACT CAGAGCAGGC TACCGGCACC TGCAGCTGAG GAACCTGCAC
    AACGAGCCTC TGGAGGTGTC CAGTCTGTTC ATGTTCAGCC GCAGGACGGA GGAGAGCCCG
    ACCGGAGGAC CACCGTCTGC GTCTGTGCTG TTCAGCACCG AGGAGAGACG ATCTGTGCAG
    CAGCACAAGG TGACCGTGCA TGGAGTCCCC GGACCTGAAC CCTTCACTGT GATCTGTGTC
    GATGAATTCA CCACAGCCAA ACAGCTCCTG GACTCTCTGT TTCCGACTGC CTCTTTTAAG
    TATATGCTGA TGGAGGAGAG AGTGTCTCTG TCGAAGGAGA AGAAGGCTCC TCAGCAGCGT
    CCTCTAGCCA ATGATGAGAG ACTGCTGAAG CTCATTCACA GCTGGCAGCC TGAGGACGGA
    TATGTGGGCA GAATATACCT CAAAACCAGA GAACAGCAGA ACTGCAGTGA GAAGACGAGC
    GTCCCGCTGG AGTCACTGGA GGAGCTCGGC AGCTTAGAGG ACGACACCTT CTTCGTGCAG
    GTCCATGATG TTTCTCCAGA ACAGCCTCAC ACCGTCATCA AAGCCCCGCG ATACAGCACA
    GCACAGGACA TCATCCAGCA GACTCTGAGC AAAGCCAAGT ATTCTCTGAG TATCCTGAGC
    AACCCCAACC CATGTGACTA CGTCCTGATG GAGGAGCTTT GTAAAGACGC GGGTGGAAAG
    AAGTCGTCCT CTGCCAAACC GTGCCAGCGT GTGCTGCAGG ACCACGAGTG TGTGTTTCAG
    GCTCAGAGCC GCTGGAAGGG GGCTGGAAAA TTCATCCTCA AACTCAAAGA GCAGTTGGCT
    CGTGAGGACA AGCGTAAAGG TGTGTCGTTC GCCAGTGAGC TGAGGAAGCT GACGGGTCGC
    AGTCGCAGTG TGACGGTCAA CACAAACAGC AACGACACTC ACAGTAAGGA AGAGAAGAGC
    GCATGTCCCA GCATGAGCTG TGTGCCGGAG ACATCGCAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45394
    Clone ID Related Accession (Same CDS sequence) XM_009306425.2
    Accession Version XM_009306425.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6759bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GTTTTCAATC
    GGCGACCTCT CAGACTCTGA AGGGGACCCA CTTGAGACAG TTAAGGATGT TGTAGACCTC
    CAGACCACCG AAGATGTTCG CGGACCCTTT AGCCATGGGA CTGAGCTCAT TCCCTGGTAC
    GTTCTGTCGC TTCAGCCGGA TATCCATCAG TTCCTGCTCC AAGGAGCAAC CGTCATCCAC
    TACGACCCCG AGAGCCACCT CACCGCCCGC TGTCTTCTCC GTCTACAGCC CGATAACTGC
    TTCCTCACCT GGTGCAAACC CCACAGTAGC TGCAGCCTAT ATGGAAGAGC TAGAACCTTC
    ATGGGACATC CAGCATCCCC AGACCACTTA CACATCGGCC AACCTGTTCA CTGCGGACTC
    TCTGACGGGC TCCTGGATCT TAATGTGGTG AAGGCTGTGT TTATGGGTCA CCCTGGAGTG
    GACGTCAACT ATGTCTGCCT GCAGCACAAA CTGTGTAACA TGAATCCTGG TGAAAATGGT
    GTCACGCTGC TATATGGACT TCACACCACA GACAACAGGC TCCTGCACTT TGTGGCCCCG
    AAACACACGG CTCGCATGCT GCACGAGGGC CTCCAGGAGC TGCTGAACTC TATTAGGAAG
    ATCAGGAAAT TCCCTGACCA GAGACTGCAA TGGCTGAGAA AGCAATATGT TAGTTTGTAC
    CAGGAGGATG GTAGATTTGA AGGACCTACA CTGGCTCATG CTATCGAGCT GTTCGGAGGG
    AGACGCTGGA ATATGGGAAC TAGCGGCCCT GGATCCGCCA GCAGAGGTGC AGAGAAGAAC
    TCGGCTCAGA AGAACAGTCC ACTGGGCATC AACTCCAATG TCAAAAAAAA GAAAAAGGCC
    TTAGTCAGGG GGGACAGTGG CGATGGAACT GATGATGAGA TGACCGCACG CAAGACCAGG
    AGTTGCAAAG AGACTTTGGG GAGGAGAGAG TCAGATGCTC TGGAAAATGT TGAGCAGGAA
    GAGGCTGAGG ACTGCAGTAC CTTTAGCCAA CCTGGACCAT TTTCCTTATC CAACAACAAA
    GGCCAGTCTC CGGGATCCTC CTCGTCCTCC TCCACCTCTT CTTCTGTTGC CTCCTTTTGC
    ACAAACTCCT CCACCCCTAC ACGTCCACAT TCCTCCCCAA TCCTCCCCAC AAACCTCAAG
    AGTCAGCCAG GGGCCTGGAG CAGTCGAAGC TGGCACGGTC GAGGGAAAGG CTGCTTCAGA
    GGTTTCCAGG ACCTCATGAT CTCCGACAGC ATCATGAGCT TTGTCGAATT TGTGGAGCTC
    TTCAAATCTT TTAGCATTCG CAGTCGTAAG GATCTGAAGG AACTATTCGA CACCTACGCC
    GTGCCCTGCA GTCGCTCTGG CCCTGAGTCT GTCCCCCTCT ACACCACACT TAGGATTGAC
    GACAAGCTAA CCGGACTGCA GCCAGATCTG GATCTCCTCA CTCGCAACGG CTCTGACCTG
    GGCCTTTTCA TTCGCACCCG CCAGCAAATG TCTGAAAACC AGAAGCAGAT TTCAGACGCC
    ATCGCAGCTG CCAGTATTGT GACCAATGGC ACTGGAGTGG AGAATTCATC GCTGGGTGTG
    CTGGGACTGG CCATCTCGCA GCTCAATGAT TTCTTGGTCA ACTGTCAGAG AGAACATCTG
    AGCTATGATG AGATCCTCAG CATCATACAG AAATTTGAGC CTTCTTCAAA CATGCGTCAG
    ATGGGGTGGA TGTCGTTTGA AGGATTCGCC AGGTTTCTAA TGGACAAGGA CAACTTTGCC
    TCCAAAAATG AGGAGTCTCA AGTGAACCTG GATGAGCTCC AGCATCCGCT GTCTTACTAT
    TACATCGAGT CCTCTCATAA CACATACCTG ACGGGGCATC AGCTGAAGGG AGAGTCTTCA
    GTCGAGCTCT ACAGCCAGGT GTTGCTGCAG GGTTGTCGCA GCGTTGAGTT GGACTGCTGG
    GATGGGGATG ACGGCATGCC TGTTATTTAC CATGGACATA CTCTTACCAC TAAGATTCCT
    TTCAAGGATG TTGTAGAGGC CATCAACCGT GCAGCATTTG TGAACTCAGA GATGCCTGTG
    ATTCTGTCCA TTGAAAACCA CTGCTCCTTA CCACAGCAGC GCAAAATGGC TGAGATTTTT
    AAGATGGTGT TTGGTGAGAA GCTGGTGACC AAGTTTCTGT TTGAAAGTGA TTTCGCGGAC
    GAGCCTTTGC TTCCGTCTCC CCTTCAGCTC CGGGGGAAAA TCCTCCTGAA GAATAAAAAG
    TTAAAAGCTC ACCAGGCTCC TGTTGACATT CTCAAACAAA AGGCGCATCA GCTTGCCCAC
    ATGCAGGCTC AGGCCAACAA CGGCACAGTC AGCACAACGC CACTGGGAAA CAATGATGAA
    GAGGAGGAGG AGGAAGACGA GTACGATTAC GATTACGAGT CGCTGTCTGA CGCTGATGTC
    CTCAATGCTT CCACAGCCTC TTATGGCCAG GAAGACAATA TTCTGGATGA CAAACCTGAA
    GGCAAGTCAT CTACGGAGAA ACTTCAGTAC GAGTCCAACG ATGAGATGCC AAAGCGGTTT
    AAAAAAGCTG GAAGCAAAGG AAAGATGTTT GACATGGAGC TGGGAGAGGA GTTTTACCTG
    CCCCAGAACG AGAAGGAGAG TAGACAGATT GCTCAAGAGC TGTCAGATCT GGTCATCTAC
    TGCCAGGCTA TCAAATTTCC AGGCCTCTCC ACCCTCTCTC CATCAGGCTC TGGAAGGGGG
    AAAGACAGAA AGAGCCGGAA ATCCATCTTT GGCAGCGCTC CTGCTCGCGG GAGCCCCGGG
    GAGCCTGTGA CGCTGGTCCG AGCTCCAGGG AAAGCCAGTC TTGAGGGCAT TCGCATGAAC
    TCGGAGGATC AATTGTGTCT GAGCCCGAGC ACCTCTCTGA GCTCCATCAT CCGCACGCCC
    AAATGCTACC ACATCTCCTC TGTGAACGAG AATGCAGCGA AGCGCTTGTG TCGCCGCTAC
    TCGCAGAAGC TGATCCAGCA CACCAGCTGC CAGCTGCTGC GCACGTACCC AGCGGCCACA
    CGCATTGACT CTGCCAACCC CAATCCGCTC ATCTTCTGGC TGCACGGAGT CCAGCTGGTG
    GCCCTCAACT ACCAGACTGA TGATCTGCCG ATGCAGCTGA ACGCGGCTCT ATTCGAGGCT
    AATGGTCACT GTGGTTTTGT GCTGAAGCCT CCAGTCCTGT GGGACAGAAG CTGTCCTCTC
    TATCAGCACT TTTACCCTCT GGACCGAGAC CTGGAGAACA TGACCCCAAC GCTATACACA
    CTCACTATTG TGTCGGGGCA GAACGTGTGT CCAGGAAACT CAAACGGCAG TCCGTGTGTC
    GAAGTGGAGG TGTTGGGAAT GCCGGCGGAC AGCTGTCATT TCCGAACTAA ACCCATACAC
    CGCAACACAC TCAATCCCAT GTGGAACGAA CACTTCCAGT TCCACGTGCA CTTTGAAGAC
    CTGGCGTTCC TGCGCATCGC TGTGGTGGAG AACAACAGCT CTCAGGTCAC CGCTCAGAGG
    ATACTTCCCC TCAAAACACT CAGAGCAGGC TACCGGCACC TGCAGCTGAG GAACCTGCAC
    AACGAGCCTC TGGAGGTGTC CAGTCTGTTC ATGTTCAGCC GCAGGACGGA GGAGAGCCCG
    ACCGGAGGAC CACCGTCTGC GTCTGTGCTG TTCAGCACCG AGGAGAGACG ATCTGTGCAG
    CAGCACAAGG TGACCGTGCA TGGAGTCCCC GGACCTGAAC CCTTCACTGT GATCTGTGTC
    GATGAATTCA CCACAGCCAA ACAGCTCCTG GACTCTCTGT TTCCGACTGC CTCTTTTAAG
    TATATGCTGA TGGAGGAGAG AGTGTCTCTG TCGAAGGAGA AGAAGGCTCC TCAGCAGCGT
    CCTCTAGCCA ATGATGAGAG ACTGCTGAAG CTCATTCACA GCTGGCAGCC TGAGGACGGA
    TATGTGGGCA GAATATACCT CAAAACCAGA GAACAGAACT GCAGTGAGAA GACGAGCGTC
    CCGCTGGAGT CACTGGAGGA GCTCGGCAGC TTAGAGGACG ACACCTTCTT CGTGCAGGTC
    CATGATGTTT CTCCAGAACA GCCTCACACC GTCATCAAAG CCCCGCGATA CAGCACAGCA
    CAGGACATCA TCCAGCAGAC TCTGAGCAAA GCCAAGTATT CTCTGAGTAT CCTGAGCAAC
    CCCAACCCAT GTGACTACGT CCTGATGGAG GAGCTTTGTA AAGACGCGGG TGGAAAGAAG
    TCGTCCTCTG CCAAACCGTG CCAGCGTGTG CTGCAGGACC ACGAGTGTGT GTTTCAGGCT
    CAGAGCCGCT GGAAGGGGGC TGGAAAATTC ATCCTCAAAC TCAAAGAGCA GTTGGCTCGT
    GAGGACAAGC GTAAAGGTGT GTCGTTCGCC AGTGAGCTGA GGAAGCTGAC GGGTCGCAGT
    CGCAGTGTGA CGGTCAACAC AAACAGCAAC GACACTCACA GTAAGGAAGA GAAGAGCGCA
    TGTCCCAGCA TGAGCTGTGT GCCGGAGACA TCGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304700.1
    CDS419..7177
    Translation

    Target ORF information:

    RefSeq Version XM_009306425.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306425.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GTTTTCAATC
    GGCGACCTCT CAGACTCTGA AGGGGACCCA CTTGAGACAG TTAAGGATGT TGTAGACCTC
    CAGACCACCG AAGATGTTCG CGGACCCTTT AGCCATGGGA CTGAGCTCAT TCCCTGGTAC
    GTTCTGTCGC TTCAGCCGGA TATCCATCAG TTCCTGCTCC AAGGAGCAAC CGTCATCCAC
    TACGACCCCG AGAGCCACCT CACCGCCCGC TGTCTTCTCC GTCTACAGCC CGATAACTGC
    TTCCTCACCT GGTGCAAACC CCACAGTAGC TGCAGCCTAT ATGGAAGAGC TAGAACCTTC
    ATGGGACATC CAGCATCCCC AGACCACTTA CACATCGGCC AACCTGTTCA CTGCGGACTC
    TCTGACGGGC TCCTGGATCT TAATGTGGTG AAGGCTGTGT TTATGGGTCA CCCTGGAGTG
    GACGTCAACT ATGTCTGCCT GCAGCACAAA CTGTGTAACA TGAATCCTGG TGAAAATGGT
    GTCACGCTGC TATATGGACT TCACACCACA GACAACAGGC TCCTGCACTT TGTGGCCCCG
    AAACACACGG CTCGCATGCT GCACGAGGGC CTCCAGGAGC TGCTGAACTC TATTAGGAAG
    ATCAGGAAAT TCCCTGACCA GAGACTGCAA TGGCTGAGAA AGCAATATGT TAGTTTGTAC
    CAGGAGGATG GTAGATTTGA AGGACCTACA CTGGCTCATG CTATCGAGCT GTTCGGAGGG
    AGACGCTGGA ATATGGGAAC TAGCGGCCCT GGATCCGCCA GCAGAGGTGC AGAGAAGAAC
    TCGGCTCAGA AGAACAGTCC ACTGGGCATC AACTCCAATG TCAAAAAAAA GAAAAAGGCC
    TTAGTCAGGG GGGACAGTGG CGATGGAACT GATGATGAGA TGACCGCACG CAAGACCAGG
    AGTTGCAAAG AGACTTTGGG GAGGAGAGAG TCAGATGCTC TGGAAAATGT TGAGCAGGAA
    GAGGCTGAGG ACTGCAGTAC CTTTAGCCAA CCTGGACCAT TTTCCTTATC CAACAACAAA
    GGCCAGTCTC CGGGATCCTC CTCGTCCTCC TCCACCTCTT CTTCTGTTGC CTCCTTTTGC
    ACAAACTCCT CCACCCCTAC ACGTCCACAT TCCTCCCCAA TCCTCCCCAC AAACCTCAAG
    AGTCAGCCAG GGGCCTGGAG CAGTCGAAGC TGGCACGGTC GAGGGAAAGG CTGCTTCAGA
    GGTTTCCAGG ACCTCATGAT CTCCGACAGC ATCATGAGCT TTGTCGAATT TGTGGAGCTC
    TTCAAATCTT TTAGCATTCG CAGTCGTAAG GATCTGAAGG AACTATTCGA CACCTACGCC
    GTGCCCTGCA GTCGCTCTGG CCCTGAGTCT GTCCCCCTCT ACACCACACT TAGGATTGAC
    GACAAGCTAA CCGGACTGCA GCCAGATCTG GATCTCCTCA CTCGCAACGG CTCTGACCTG
    GGCCTTTTCA TTCGCACCCG CCAGCAAATG TCTGAAAACC AGAAGCAGAT TTCAGACGCC
    ATCGCAGCTG CCAGTATTGT GACCAATGGC ACTGGAGTGG AGAATTCATC GCTGGGTGTG
    CTGGGACTGG CCATCTCGCA GCTCAATGAT TTCTTGGTCA ACTGTCAGAG AGAACATCTG
    AGCTATGATG AGATCCTCAG CATCATACAG AAATTTGAGC CTTCTTCAAA CATGCGTCAG
    ATGGGGTGGA TGTCGTTTGA AGGATTCGCC AGGTTTCTAA TGGACAAGGA CAACTTTGCC
    TCCAAAAATG AGGAGTCTCA AGTGAACCTG GATGAGCTCC AGCATCCGCT GTCTTACTAT
    TACATCGAGT CCTCTCATAA CACATACCTG ACGGGGCATC AGCTGAAGGG AGAGTCTTCA
    GTCGAGCTCT ACAGCCAGGT GTTGCTGCAG GGTTGTCGCA GCGTTGAGTT GGACTGCTGG
    GATGGGGATG ACGGCATGCC TGTTATTTAC CATGGACATA CTCTTACCAC TAAGATTCCT
    TTCAAGGATG TTGTAGAGGC CATCAACCGT GCAGCATTTG TGAACTCAGA GATGCCTGTG
    ATTCTGTCCA TTGAAAACCA CTGCTCCTTA CCACAGCAGC GCAAAATGGC TGAGATTTTT
    AAGATGGTGT TTGGTGAGAA GCTGGTGACC AAGTTTCTGT TTGAAAGTGA TTTCGCGGAC
    GAGCCTTTGC TTCCGTCTCC CCTTCAGCTC CGGGGGAAAA TCCTCCTGAA GAATAAAAAG
    TTAAAAGCTC ACCAGGCTCC TGTTGACATT CTCAAACAAA AGGCGCATCA GCTTGCCCAC
    ATGCAGGCTC AGGCCAACAA CGGCACAGTC AGCACAACGC CACTGGGAAA CAATGATGAA
    GAGGAGGAGG AGGAAGACGA GTACGATTAC GATTACGAGT CGCTGTCTGA CGCTGATGTC
    CTCAATGCTT CCACAGCCTC TTATGGCCAG GAAGACAATA TTCTGGATGA CAAACCTGAA
    GGCAAGTCAT CTACGGAGAA ACTTCAGTAC GAGTCCAACG ATGAGATGCC AAAGCGGTTT
    AAAAAAGCTG GAAGCAAAGG AAAGATGTTT GACATGGAGC TGGGAGAGGA GTTTTACCTG
    CCCCAGAACG AGAAGGAGAG TAGACAGATT GCTCAAGAGC TGTCAGATCT GGTCATCTAC
    TGCCAGGCTA TCAAATTTCC AGGCCTCTCC ACCCTCTCTC CATCAGGCTC TGGAAGGGGG
    AAAGACAGAA AGAGCCGGAA ATCCATCTTT GGCAGCGCTC CTGCTCGCGG GAGCCCCGGG
    GAGCCTGTGA CGCTGGTCCG AGCTCCAGGG AAAGCCAGTC TTGAGGGCAT TCGCATGAAC
    TCGGAGGATC AATTGTGTCT GAGCCCGAGC ACCTCTCTGA GCTCCATCAT CCGCACGCCC
    AAATGCTACC ACATCTCCTC TGTGAACGAG AATGCAGCGA AGCGCTTGTG TCGCCGCTAC
    TCGCAGAAGC TGATCCAGCA CACCAGCTGC CAGCTGCTGC GCACGTACCC AGCGGCCACA
    CGCATTGACT CTGCCAACCC CAATCCGCTC ATCTTCTGGC TGCACGGAGT CCAGCTGGTG
    GCCCTCAACT ACCAGACTGA TGATCTGCCG ATGCAGCTGA ACGCGGCTCT ATTCGAGGCT
    AATGGTCACT GTGGTTTTGT GCTGAAGCCT CCAGTCCTGT GGGACAGAAG CTGTCCTCTC
    TATCAGCACT TTTACCCTCT GGACCGAGAC CTGGAGAACA TGACCCCAAC GCTATACACA
    CTCACTATTG TGTCGGGGCA GAACGTGTGT CCAGGAAACT CAAACGGCAG TCCGTGTGTC
    GAAGTGGAGG TGTTGGGAAT GCCGGCGGAC AGCTGTCATT TCCGAACTAA ACCCATACAC
    CGCAACACAC TCAATCCCAT GTGGAACGAA CACTTCCAGT TCCACGTGCA CTTTGAAGAC
    CTGGCGTTCC TGCGCATCGC TGTGGTGGAG AACAACAGCT CTCAGGTCAC CGCTCAGAGG
    ATACTTCCCC TCAAAACACT CAGAGCAGGC TACCGGCACC TGCAGCTGAG GAACCTGCAC
    AACGAGCCTC TGGAGGTGTC CAGTCTGTTC ATGTTCAGCC GCAGGACGGA GGAGAGCCCG
    ACCGGAGGAC CACCGTCTGC GTCTGTGCTG TTCAGCACCG AGGAGAGACG ATCTGTGCAG
    CAGCACAAGG TGACCGTGCA TGGAGTCCCC GGACCTGAAC CCTTCACTGT GATCTGTGTC
    GATGAATTCA CCACAGCCAA ACAGCTCCTG GACTCTCTGT TTCCGACTGC CTCTTTTAAG
    TATATGCTGA TGGAGGAGAG AGTGTCTCTG TCGAAGGAGA AGAAGGCTCC TCAGCAGCGT
    CCTCTAGCCA ATGATGAGAG ACTGCTGAAG CTCATTCACA GCTGGCAGCC TGAGGACGGA
    TATGTGGGCA GAATATACCT CAAAACCAGA GAACAGAACT GCAGTGAGAA GACGAGCGTC
    CCGCTGGAGT CACTGGAGGA GCTCGGCAGC TTAGAGGACG ACACCTTCTT CGTGCAGGTC
    CATGATGTTT CTCCAGAACA GCCTCACACC GTCATCAAAG CCCCGCGATA CAGCACAGCA
    CAGGACATCA TCCAGCAGAC TCTGAGCAAA GCCAAGTATT CTCTGAGTAT CCTGAGCAAC
    CCCAACCCAT GTGACTACGT CCTGATGGAG GAGCTTTGTA AAGACGCGGG TGGAAAGAAG
    TCGTCCTCTG CCAAACCGTG CCAGCGTGTG CTGCAGGACC ACGAGTGTGT GTTTCAGGCT
    CAGAGCCGCT GGAAGGGGGC TGGAAAATTC ATCCTCAAAC TCAAAGAGCA GTTGGCTCGT
    GAGGACAAGC GTAAAGGTGT GTCGTTCGCC AGTGAGCTGA GGAAGCTGAC GGGTCGCAGT
    CGCAGTGTGA CGGTCAACAC AAACAGCAAC GACACTCACA GTAAGGAAGA GAAGAGCGCA
    TGTCCCAGCA TGAGCTGTGT GCCGGAGACA TCGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45395
    Clone ID Related Accession (Same CDS sequence) XM_009306426.2
    Accession Version XM_009306426.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GTTTTCAATC
    GGCGACCTCT CAGACTCTGA AGGGGACCCA CTTGAGACAG TTAAGGATGT TGTAGACCTC
    CAGACCACCG AAGATGTTCG CGGACCCTTT AGCCATGGGA CTGAGCTCAT TCCCTGGTAC
    GTTCTGTCGC TTCAGCCGGA TATCCATCAG TTCCTGCTCC AAGGAGCAAC CGTCATCCAC
    TACGACCCCG AGAGCCACCT CACCGCCCGC TGTCTTCTCC GTCTACAGCC CGATAACTGC
    TTCCTCACCT GGTGCAAACC CCACAGTAGC TGCAGCCTAT ATGGAAGAGC TAGAACCTTC
    ATGGGACATC CAGCATCCCC AGACCACTTA CACATCGGCC AACCTGTTCA CTGCGGACTC
    TCTGACGGGC TCCTGGATCT TAATGTGGTG AAGGCTGTGT TTATGGGTCA CCCTGGAGTG
    GACGTCAACT ATGTCTGCCT GCAGCACAAA CTGTGTAACA TGAATCCTGG TGAAAATGGT
    GTCACGCTGC TATATGGACT TCACACCACA GACAACAGGC TCCTGCACTT TGTGGCCCCG
    AAACACACGG CTCGCATGCT GCACGAGGGC CTCCAGGAGC TGCTGAACTC TATTAGGAAG
    ATCAGGAAAT TCCCTGACCA GAGACTGCAA TGGCTGAGAA AGCAATATGT TAGTTTGTAC
    CAGGAGGATG GTAGATTTGA AGGACCTACA CTGGCTCATG CTATCGAGCT GTTCGGAGGG
    AGACGCTGGA ATATGGGAAC TAGCGGCCCT GGATCCGCCA GCAGAGGTGC AGAGAAGAAC
    TCGGCTCAGA AGAACAGTCC ACTGGGCATC AACTCCAATG TCAAAAAAAA GAAAAAGGCC
    TTAGTCAGGG GGGACAGTGG CGATGGAACT GATGATGAGA TGACCGCACG CAAGACCAGG
    AGTTGCAAAG AGACTTTGGG GAGGAGAGAG TCAGATGCTC TGGAAAATGT TGAGCAGGAA
    GAGGCTGAGG ACTGCAGTAC CTTTAGCCAA CCTGGACCAT TTTCCTTATC CAACAACAAA
    GGCCAGTCTC CGGGATCCTC CTCGTCCTCC TCCACCTCTT CTTCTGTTGC CTCCTTTTGC
    ACAAACTCCT CCACCCCTAC ACGTCCACAT TCCTCCCCAA TCCTCCCCAC AAACCTCAAG
    AGTCAGCCAG GGGCCTGGAG CAGTCGAAGC TGGCACGGTC GAGGGAAAGG CTGCTTCAGA
    GGTTTCCAGG ACCTCATGAT CTCCGACAGC ATCATGAGCT TTGTCGAATT TGTGGAGCTC
    TTCAAATCTT TTAGCATTCG CAGTCGTAAG GATCTGAAGG AACTATTCGA CACCTACGCC
    GTGCCCTGCA GTCGCTCTGG CCCTGAGTCT GTCCCCCTCT ACACCACACT TAGGATTGAC
    GACAAGCTAA CCGGACTGCA GCCAGATCTG GATCTCCTCA CTCGCAACGG CTCTGACCTG
    GGCCTTTTCA TTCGCACCCG CCAGCAAATG TCTGAAAACC AGAAGCAGAT TTCAGACGCC
    ATCGCAGCTG CCAGTATTGT GACCAATGGC ACTGGAGTGG AGAATTCATC GCTGGGTGTG
    CTGGGACTGG CCATCTCGCA GCTCAATGAT TTCTTGGTCA ACTGTCAGAG AGAACATCTG
    AGCTATGATG AGATCCTCAG CATCATACAG AAATTTGAGC CTTCTTCAAA CATGCGTCAG
    ATGGGGTGGA TGTCGTTTGA AGGATTCGCC AGGTTTCTAA TGGACAAGGA CAACTTTGCC
    TCCAAAAATG AGGAGTCTCA AGTGAACCTG GATGAGCTCC AGCATCCGCT GTCTTACTAT
    TACATCGAGT CCTCTCATAA CACATACCTG ACGGGGCATC AGCTGAAGGG AGAGTCTTCA
    GTCGAGCTCT ACAGCCAGGT GTTGCTGCAG GGTTGTCGCA GCGTTGAGTT GGACTGCTGG
    GATGGGGATG ACGGCATGCC TGTTATTTAC CATGGACATA CTCTTACCAC TAAGATTCCT
    TTCAAGGATG TTGTAGAGGC CATCAACCGT GCAGCATTTG TGAACTCAGA GATGCCTGTG
    ATTCTGTCCA TTGAAAACCA CTGCTCCTTA CCACAGCAGC GCAAAATGGC TGAGATTTTT
    AAGATGGTGT TTGGTGAGAA GCTGGTGACC AAGTTTCTGT TTGAAAGTGA TTTCGCGGAC
    GAGCCTTTGC TTCCGTCTCC CCTTCAGCTC CGGGGGAAAA TCCTCCTGAA GAATAAAAAG
    TTAAAAGCTC ACCAGGCTCC TGTTGACATT CTCAAACAAA AGGCGCATCA GCTTGCCCAC
    ATGCAGGCTC AGGCCAACAA CGGCACAGTC AGCACAACGC CACTGGGAAA CAATGATGAA
    GAGGAGGAGG AGGAAGACGA GTACGATTAC GATTACGAGT CGCTGTCTGA CGACAATATT
    CTGGATGACA AACCTGAAGG CAAGTCATCT ACGGAGAAAC TTCAGTACGA GTCCAACGAT
    GAGATGCCAA AGCGGTTTAA AAAAGCTGGA AGCAAAGGAA AGATGTTTGA CATGGAGCTG
    GGAGAGGAGT TTTACCTGCC CCAGAACGAG AAGGAGAGTA GACAGATTGC TCAAGAGCTG
    TCAGATCTGG TCATCTACTG CCAGGCTATC AAATTTCCAG GCCTCTCCAC CCTCTCTCCA
    TCAGGCTCTG GAAGGGGGAA AGACAGAAAG AGCCGGAAAT CCATCTTTGG CAGCGCTCCT
    GCTCGCGGGA GCCCCGGGGA GCCTGTGACG CTGGTCCGAG CTCCAGGGAA AGCCAGTCTT
    GAGGGCATTC GCATGAACTC GGAGGATCAA TTGTGTCTGA GCCCGAGCAC CTCTCTGAGC
    TCCATCATCC GCACGCCCAA ATGCTACCAC ATCTCCTCTG TGAACGAGAA TGCAGCGAAG
    CGCTTGTGTC GCCGCTACTC GCAGAAGCTG ATCCAGCACA CCAGCTGCCA GCTGCTGCGC
    ACGTACCCAG CGGCCACACG CATTGACTCT GCCAACCCCA ATCCGCTCAT CTTCTGGCTG
    CACGGAGTCC AGCTGGTGGC CCTCAACTAC CAGACTGATG ATCTGCCGAT GCAGCTGAAC
    GCGGCTCTAT TCGAGGCTAA TGGTCACTGT GGTTTTGTGC TGAAGCCTCC AGTCCTGTGG
    GACAGAAGCT GTCCTCTCTA TCAGCACTTT TACCCTCTGG ACCGAGACCT GGAGAACATG
    ACCCCAACGC TATACACACT CACTATTGTG TCGGGGCAGA ACGTGTGTCC AGGAAACTCA
    AACGGCAGTC CGTGTGTCGA AGTGGAGGTG TTGGGAATGC CGGCGGACAG CTGTCATTTC
    CGAACTAAAC CCATACACCG CAACACACTC AATCCCATGT GGAACGAACA CTTCCAGTTC
    CACGTGCACT TTGAAGACCT GGCGTTCCTG CGCATCGCTG TGGTGGAGAA CAACAGCTCT
    CAGGTCACCG CTCAGAGGAT ACTTCCCCTC AAAACACTCA GAGCAGGCTA CCGGCACCTG
    CAGCTGAGGA ACCTGCACAA CGAGCCTCTG GAGGTGTCCA GTCTGTTCAT GTTCAGCCGC
    AGGACGGAGG AGAGCCCGAC CGGAGGACCA CCGTCTGCGT CTGTGCTGTT CAGCACCGAG
    GAGAGACGAT CTGTGCAGCA GCACAAGGTG ACCGTGCATG GAGTCCCCGG ACCTGAACCC
    TTCACTGTGA TCTGTGTCGA TGAATTCACC ACAGCCAAAC AGCTCCTGGA CTCTCTGTTT
    CCGACTGCCT CTTTTAAGTA TATGCTGATG GAGGAGAGAG TGTCTCTGTC GAAGGAGAAG
    AAGGCTCCTC AGCAGCGTCC TCTAGCCAAT GATGAGAGAC TGCTGAAGCT CATTCACAGC
    TGGCAGCCTG AGGACGGATA TGTGGGCAGA ATATACCTCA AAACCAGAGA ACAGCAGAAC
    TGCAGTGAGA AGACGAGCGT CCCGCTGGAG TCACTGGAGG AGCTCGGCAG CTTAGAGGAC
    GACACCTTCT TCGTGCAGGT CCATGATGTT TCTCCAGAAC AGCCTCACAC CGTCATCAAA
    GCCCCGCGAT ACAGCACAGC ACAGGACATC ATCCAGCAGA CTCTGAGCAA AGCCAAGTAT
    TCTCTGAGTA TCCTGAGCAA CCCCAACCCA TGTGACTACG TCCTGATGGA GGAGCTTTGT
    AAAGACGCGG GTGGAAAGAA GTCGTCCTCT GCCAAACCGT GCCAGCGTGT GCTGCAGGAC
    CACGAGTGTG TGTTTCAGGC TCAGAGCCGC TGGAAGGGGG CTGGAAAATT CATCCTCAAA
    CTCAAAGAGC AGTTGGCTCG TGAGGACAAG CGTAAAGGTG TGTCGTTCGC CAGTGAGCTG
    AGGAAGCTGA CGGGTCGCAG TCGCAGTGTG ACGGTCAACA CAAACAGCAA CGACACTCAC
    AGTAAGGAAG AGAAGAGCGC ATGTCCCAGC ATGAGCTGTG TGCCGGAGAC ATCGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304701.1
    CDS419..7138
    Translation

    Target ORF information:

    RefSeq Version XM_009306426.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306426.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GTTTTCAATC
    GGCGACCTCT CAGACTCTGA AGGGGACCCA CTTGAGACAG TTAAGGATGT TGTAGACCTC
    CAGACCACCG AAGATGTTCG CGGACCCTTT AGCCATGGGA CTGAGCTCAT TCCCTGGTAC
    GTTCTGTCGC TTCAGCCGGA TATCCATCAG TTCCTGCTCC AAGGAGCAAC CGTCATCCAC
    TACGACCCCG AGAGCCACCT CACCGCCCGC TGTCTTCTCC GTCTACAGCC CGATAACTGC
    TTCCTCACCT GGTGCAAACC CCACAGTAGC TGCAGCCTAT ATGGAAGAGC TAGAACCTTC
    ATGGGACATC CAGCATCCCC AGACCACTTA CACATCGGCC AACCTGTTCA CTGCGGACTC
    TCTGACGGGC TCCTGGATCT TAATGTGGTG AAGGCTGTGT TTATGGGTCA CCCTGGAGTG
    GACGTCAACT ATGTCTGCCT GCAGCACAAA CTGTGTAACA TGAATCCTGG TGAAAATGGT
    GTCACGCTGC TATATGGACT TCACACCACA GACAACAGGC TCCTGCACTT TGTGGCCCCG
    AAACACACGG CTCGCATGCT GCACGAGGGC CTCCAGGAGC TGCTGAACTC TATTAGGAAG
    ATCAGGAAAT TCCCTGACCA GAGACTGCAA TGGCTGAGAA AGCAATATGT TAGTTTGTAC
    CAGGAGGATG GTAGATTTGA AGGACCTACA CTGGCTCATG CTATCGAGCT GTTCGGAGGG
    AGACGCTGGA ATATGGGAAC TAGCGGCCCT GGATCCGCCA GCAGAGGTGC AGAGAAGAAC
    TCGGCTCAGA AGAACAGTCC ACTGGGCATC AACTCCAATG TCAAAAAAAA GAAAAAGGCC
    TTAGTCAGGG GGGACAGTGG CGATGGAACT GATGATGAGA TGACCGCACG CAAGACCAGG
    AGTTGCAAAG AGACTTTGGG GAGGAGAGAG TCAGATGCTC TGGAAAATGT TGAGCAGGAA
    GAGGCTGAGG ACTGCAGTAC CTTTAGCCAA CCTGGACCAT TTTCCTTATC CAACAACAAA
    GGCCAGTCTC CGGGATCCTC CTCGTCCTCC TCCACCTCTT CTTCTGTTGC CTCCTTTTGC
    ACAAACTCCT CCACCCCTAC ACGTCCACAT TCCTCCCCAA TCCTCCCCAC AAACCTCAAG
    AGTCAGCCAG GGGCCTGGAG CAGTCGAAGC TGGCACGGTC GAGGGAAAGG CTGCTTCAGA
    GGTTTCCAGG ACCTCATGAT CTCCGACAGC ATCATGAGCT TTGTCGAATT TGTGGAGCTC
    TTCAAATCTT TTAGCATTCG CAGTCGTAAG GATCTGAAGG AACTATTCGA CACCTACGCC
    GTGCCCTGCA GTCGCTCTGG CCCTGAGTCT GTCCCCCTCT ACACCACACT TAGGATTGAC
    GACAAGCTAA CCGGACTGCA GCCAGATCTG GATCTCCTCA CTCGCAACGG CTCTGACCTG
    GGCCTTTTCA TTCGCACCCG CCAGCAAATG TCTGAAAACC AGAAGCAGAT TTCAGACGCC
    ATCGCAGCTG CCAGTATTGT GACCAATGGC ACTGGAGTGG AGAATTCATC GCTGGGTGTG
    CTGGGACTGG CCATCTCGCA GCTCAATGAT TTCTTGGTCA ACTGTCAGAG AGAACATCTG
    AGCTATGATG AGATCCTCAG CATCATACAG AAATTTGAGC CTTCTTCAAA CATGCGTCAG
    ATGGGGTGGA TGTCGTTTGA AGGATTCGCC AGGTTTCTAA TGGACAAGGA CAACTTTGCC
    TCCAAAAATG AGGAGTCTCA AGTGAACCTG GATGAGCTCC AGCATCCGCT GTCTTACTAT
    TACATCGAGT CCTCTCATAA CACATACCTG ACGGGGCATC AGCTGAAGGG AGAGTCTTCA
    GTCGAGCTCT ACAGCCAGGT GTTGCTGCAG GGTTGTCGCA GCGTTGAGTT GGACTGCTGG
    GATGGGGATG ACGGCATGCC TGTTATTTAC CATGGACATA CTCTTACCAC TAAGATTCCT
    TTCAAGGATG TTGTAGAGGC CATCAACCGT GCAGCATTTG TGAACTCAGA GATGCCTGTG
    ATTCTGTCCA TTGAAAACCA CTGCTCCTTA CCACAGCAGC GCAAAATGGC TGAGATTTTT
    AAGATGGTGT TTGGTGAGAA GCTGGTGACC AAGTTTCTGT TTGAAAGTGA TTTCGCGGAC
    GAGCCTTTGC TTCCGTCTCC CCTTCAGCTC CGGGGGAAAA TCCTCCTGAA GAATAAAAAG
    TTAAAAGCTC ACCAGGCTCC TGTTGACATT CTCAAACAAA AGGCGCATCA GCTTGCCCAC
    ATGCAGGCTC AGGCCAACAA CGGCACAGTC AGCACAACGC CACTGGGAAA CAATGATGAA
    GAGGAGGAGG AGGAAGACGA GTACGATTAC GATTACGAGT CGCTGTCTGA CGACAATATT
    CTGGATGACA AACCTGAAGG CAAGTCATCT ACGGAGAAAC TTCAGTACGA GTCCAACGAT
    GAGATGCCAA AGCGGTTTAA AAAAGCTGGA AGCAAAGGAA AGATGTTTGA CATGGAGCTG
    GGAGAGGAGT TTTACCTGCC CCAGAACGAG AAGGAGAGTA GACAGATTGC TCAAGAGCTG
    TCAGATCTGG TCATCTACTG CCAGGCTATC AAATTTCCAG GCCTCTCCAC CCTCTCTCCA
    TCAGGCTCTG GAAGGGGGAA AGACAGAAAG AGCCGGAAAT CCATCTTTGG CAGCGCTCCT
    GCTCGCGGGA GCCCCGGGGA GCCTGTGACG CTGGTCCGAG CTCCAGGGAA AGCCAGTCTT
    GAGGGCATTC GCATGAACTC GGAGGATCAA TTGTGTCTGA GCCCGAGCAC CTCTCTGAGC
    TCCATCATCC GCACGCCCAA ATGCTACCAC ATCTCCTCTG TGAACGAGAA TGCAGCGAAG
    CGCTTGTGTC GCCGCTACTC GCAGAAGCTG ATCCAGCACA CCAGCTGCCA GCTGCTGCGC
    ACGTACCCAG CGGCCACACG CATTGACTCT GCCAACCCCA ATCCGCTCAT CTTCTGGCTG
    CACGGAGTCC AGCTGGTGGC CCTCAACTAC CAGACTGATG ATCTGCCGAT GCAGCTGAAC
    GCGGCTCTAT TCGAGGCTAA TGGTCACTGT GGTTTTGTGC TGAAGCCTCC AGTCCTGTGG
    GACAGAAGCT GTCCTCTCTA TCAGCACTTT TACCCTCTGG ACCGAGACCT GGAGAACATG
    ACCCCAACGC TATACACACT CACTATTGTG TCGGGGCAGA ACGTGTGTCC AGGAAACTCA
    AACGGCAGTC CGTGTGTCGA AGTGGAGGTG TTGGGAATGC CGGCGGACAG CTGTCATTTC
    CGAACTAAAC CCATACACCG CAACACACTC AATCCCATGT GGAACGAACA CTTCCAGTTC
    CACGTGCACT TTGAAGACCT GGCGTTCCTG CGCATCGCTG TGGTGGAGAA CAACAGCTCT
    CAGGTCACCG CTCAGAGGAT ACTTCCCCTC AAAACACTCA GAGCAGGCTA CCGGCACCTG
    CAGCTGAGGA ACCTGCACAA CGAGCCTCTG GAGGTGTCCA GTCTGTTCAT GTTCAGCCGC
    AGGACGGAGG AGAGCCCGAC CGGAGGACCA CCGTCTGCGT CTGTGCTGTT CAGCACCGAG
    GAGAGACGAT CTGTGCAGCA GCACAAGGTG ACCGTGCATG GAGTCCCCGG ACCTGAACCC
    TTCACTGTGA TCTGTGTCGA TGAATTCACC ACAGCCAAAC AGCTCCTGGA CTCTCTGTTT
    CCGACTGCCT CTTTTAAGTA TATGCTGATG GAGGAGAGAG TGTCTCTGTC GAAGGAGAAG
    AAGGCTCCTC AGCAGCGTCC TCTAGCCAAT GATGAGAGAC TGCTGAAGCT CATTCACAGC
    TGGCAGCCTG AGGACGGATA TGTGGGCAGA ATATACCTCA AAACCAGAGA ACAGCAGAAC
    TGCAGTGAGA AGACGAGCGT CCCGCTGGAG TCACTGGAGG AGCTCGGCAG CTTAGAGGAC
    GACACCTTCT TCGTGCAGGT CCATGATGTT TCTCCAGAAC AGCCTCACAC CGTCATCAAA
    GCCCCGCGAT ACAGCACAGC ACAGGACATC ATCCAGCAGA CTCTGAGCAA AGCCAAGTAT
    TCTCTGAGTA TCCTGAGCAA CCCCAACCCA TGTGACTACG TCCTGATGGA GGAGCTTTGT
    AAAGACGCGG GTGGAAAGAA GTCGTCCTCT GCCAAACCGT GCCAGCGTGT GCTGCAGGAC
    CACGAGTGTG TGTTTCAGGC TCAGAGCCGC TGGAAGGGGG CTGGAAAATT CATCCTCAAA
    CTCAAAGAGC AGTTGGCTCG TGAGGACAAG CGTAAAGGTG TGTCGTTCGC CAGTGAGCTG
    AGGAAGCTGA CGGGTCGCAG TCGCAGTGTG ACGGTCAACA CAAACAGCAA CGACACTCAC
    AGTAAGGAAG AGAAGAGCGC ATGTCCCAGC ATGAGCTGTG TGCCGGAGAC ATCGCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45397
    Clone ID Related Accession (Same CDS sequence) XM_009306428.2
    Accession Version XM_009306428.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GTTTTCAATC
    GGCGACCTCT CAGACTCTGA AGGGGACCCA CTTGAGACAG TTAAGGATGT TGTAGACCTC
    CAGACCACCG AAGATGTTCG CGGACCCTTT AGCCATGGGA CTGAGCTCAT TCCCTGGTAC
    GTTCTGTCGC TTCAGCCGGA TATCCATCAG TTCCTGCTCC AAGGAGCAAC CGTCATCCAC
    TACGACCCCG AGAGCCACCT CACCGCCCGC TGTCTTCTCC GTCTACAGCC CGATAACTGC
    TTCCTCACCT GGTGCAAACC CCACAGTAGC TGCAGCCTAT ATGGAAGAGC TAGAACCTTC
    ATGGGACATC CAGCATCCCC AGACCACTTA CACATCGGCC AACCTGTTCA CTGCGGACTC
    TCTGACGGGC TCCTGGATCT TAATGTGGTG AAGGCTGTGT TTATGGGTCA CCCTGGAGTG
    GACGTCAACT ATGTCTGCCT GCAGCACAAA CTGTGTAACA TGAATCCTGG TGAAAATGGT
    GTCACGCTGC TATATGGACT TCACACCACA GACAACAGGC TCCTGCACTT TGTGGCCCCG
    AAACACACGG CTCGCATGCT GCACGAGGGC CTCCAGGAGC TGCTGAACTC TATTAGGAAG
    ATCAGGAAAT TCCCTGACCA GAGACTGCAA TGGCTGAGAA AGCAATATGT TAGTTTGTAC
    CAGGAGGATG GTAGATTTGA AGGACCTACA CTGGCTCATG CTATCGAGCT GTTCGGAGGG
    AGACGCTGGA ATATGGGAAC TAGCGGCCCT GGATCCGCCA GCAGAGGTGC AGAGAAGAAC
    TCGGCTCAGA AGAACAGTCC ACTGGGCATC AACTCCAATG TCAAAAAAAA GAAAAAGGCC
    TTAGTCAGGG GGGACAGTGG CGATGGAACT GATGATGAGA TGACCGCACG CAAGACCAGG
    AGTTGCAAAG AGACTTTGGG GAGGAGAGAG TCAGATGCTC TGGAAAATGT TGAGCAGGAA
    GAGGCTGAGG ACTGCAGTAC CTTTAGCCAA CCTGGACCAT TTTCCTTATC CAACAACAAA
    GGCCAGTCTC CGGGATCCTC CTCGTCCTCC TCCACCTCTT CTTCTGTTGC CTCCTTTTGC
    ACAAACTCCT CCACCCCTAC ACGTCCACAT TCCTCCCCAA TCCTCCCCAC AAACCTCAAG
    AGTCAGCCAG GGGCCTGGAG CAGTCGAAGC TGGCACGGTC GAGGGAAAGG CTGCTTCAGA
    GGTTTCCAGG ACCTCATGAT CTCCGACAGC ATCATGAGCT TTGTCGAATT TGTGGAGCTC
    TTCAAATCTT TTAGCATTCG CAGTCGTAAG GATCTGAAGG AACTATTCGA CACCTACGCC
    GTGCCCTGCA GTCGCTCTGG CCCTGAGTCT GTCCCCCTCT ACACCACACT TAGGATTGAC
    GACAAGCTAA CCGGACTGCA GCCAGATCTG GATCTCCTCA CTCGCAACGG CTCTGACCTG
    GGCCTTTTCA TTCGCACCCG CCAGCAAATG TCTGAAAACC AGAAGCAGAT TTCAGACGCC
    ATCGCAGCTG CCAGTATTGT GACCAATGGC ACTGGAGTGG AGAATTCATC GCTGGGTGTG
    CTGGGACTGG CCATCTCGCA GCTCAATGAT TTCTTGGTCA ACTGTCAGAG AGAACATCTG
    AGCTATGATG AGATCCTCAG CATCATACAG AAATTTGAGC CTTCTTCAAA CATGCGTCAG
    ATGGGGTGGA TGTCGTTTGA AGGATTCGCC AGGTTTCTAA TGGACAAGGA CAACTTTGCC
    TCCAAAAATG AGGAGTCTCA AGTGAACCTG GATGAGCTCC AGCATCCGCT GTCTTACTAT
    TACATCGAGT CCTCTCATAA CACATACCTG ACGGGGCATC AGCTGAAGGG AGAGTCTTCA
    GTCGAGCTCT ACAGCCAGGT GTTGCTGCAG GGTTGTCGCA GCGTTGAGTT GGACTGCTGG
    GATGGGGATG ACGGCATGCC TGTTATTTAC CATGGACATA CTCTTACCAC TAAGATTCCT
    TTCAAGGATG TTGTAGAGGC CATCAACCGT GCAGCATTTG TGAACTCAGA GATGCCTGTG
    ATTCTGTCCA TTGAAAACCA CTGCTCCTTA CCACAGCAGC GCAAAATGGC TGAGATTTTT
    AAGATGGTGT TTGGTGAGAA GCTGGTGACC AAGTTTCTGT TTGAAAGTGA TTTCGCGGAC
    GAGCCTTTGC TTCCGTCTCC CCTTCAGCTC CGGGGGAAAA TCCTCCTGAA GAATAAAAAG
    TTAAAAGCTC ACCAGGCTCC TGTTGACATT CTCAAACAAA AGGCGCATCA GCTTGCCCAC
    ATGCAGGCTC AGGCCAACAA CGGCACAGTC AGCACAACGC CACTGGGAAA CAATGATGAA
    GAGGAGGAGG AGGAAGACGA GTACGATTAC GATTACGAGT CGCTGTCTGA CGACAATATT
    CTGGATGACA AACCTGAAGG CAAGTCATCT ACGGAGAAAC TTCAGTACGA GTCCAACGAT
    GAGATGCCAA AGCGGTTTAA AAAAGCTGGA AGCAAAGGAA AGATGTTTGA CATGGAGCTG
    GGAGAGGAGT TTTACCTGCC CCAGAACGAG AAGGAGAGTA GACAGATTGC TCAAGAGCTG
    TCAGATCTGG TCATCTACTG CCAGGCTATC AAATTTCCAG GCCTCTCCAC CCTCTCTCCA
    TCAGGCTCTG GAAGGGGGAA AGACAGAAAG AGCCGGAAAT CCATCTTTGG CAGCGCTCCT
    GCTCGCGGGA GCCCCGGGGA GCCTGTGACG CTGGTCCGAG CTCCAGGGAA AGCCAGTCTT
    GAGGGCATTC GCATGAACTC GGAGGATCAA TTGTGTCTGA GCCCGAGCAC CTCTCTGAGC
    TCCATCATCC GCACGCCCAA ATGCTACCAC ATCTCCTCTG TGAACGAGAA TGCAGCGAAG
    CGCTTGTGTC GCCGCTACTC GCAGAAGCTG ATCCAGCACA CCAGCTGCCA GCTGCTGCGC
    ACGTACCCAG CGGCCACACG CATTGACTCT GCCAACCCCA ATCCGCTCAT CTTCTGGCTG
    CACGGAGTCC AGCTGGTGGC CCTCAACTAC CAGACTGATG ATCTGCCGAT GCAGCTGAAC
    GCGGCTCTAT TCGAGGCTAA TGGTCACTGT GGTTTTGTGC TGAAGCCTCC AGTCCTGTGG
    GACAGAAGCT GTCCTCTCTA TCAGCACTTT TACCCTCTGG ACCGAGACCT GGAGAACATG
    ACCCCAACGC TATACACACT CACTATTGTG TCGGGGCAGA ACGTGTGTCC AGGAAACTCA
    AACGGCAGTC CGTGTGTCGA AGTGGAGGTG TTGGGAATGC CGGCGGACAG CTGTCATTTC
    CGAACTAAAC CCATACACCG CAACACACTC AATCCCATGT GGAACGAACA CTTCCAGTTC
    CACGTGCACT TTGAAGACCT GGCGTTCCTG CGCATCGCTG TGGTGGAGAA CAACAGCTCT
    CAGGTCACCG CTCAGAGGAT ACTTCCCCTC AAAACACTCA GAGCAGGCTA CCGGCACCTG
    CAGCTGAGGA ACCTGCACAA CGAGCCTCTG GAGGTGTCCA GTCTGTTCAT GTTCAGCCGC
    AGGACGGAGG AGAGCCCGAC CGGAGGACCA CCGTCTGCGT CTGTGCTGTT CAGCACCGAG
    GAGAGACGAT CTGTGCAGCA GCACAAGGTG ACCGTGCATG GAGTCCCCGG ACCTGAACCC
    TTCACTGTGA TCTGTGTCGA TGAATTCACC ACAGCCAAAC AGCTCCTGGA CTCTCTGTTT
    CCGACTGCCT CTTTTAAGTA TATGCTGATG GAGGAGAGAG TGTCTCTGTC GAAGGAGAAG
    AAGGCTCCTC AGCAGCGTCC TCTAGCCAAT GATGAGAGAC TGCTGAAGCT CATTCACAGC
    TGGCAGCCTG AGGACGGATA TGTGGGCAGA ATATACCTCA AAACCAGAGA ACAGAACTGC
    AGTGAGAAGA CGAGCGTCCC GCTGGAGTCA CTGGAGGAGC TCGGCAGCTT AGAGGACGAC
    ACCTTCTTCG TGCAGGTCCA TGATGTTTCT CCAGAACAGC CTCACACCGT CATCAAAGCC
    CCGCGATACA GCACAGCACA GGACATCATC CAGCAGACTC TGAGCAAAGC CAAGTATTCT
    CTGAGTATCC TGAGCAACCC CAACCCATGT GACTACGTCC TGATGGAGGA GCTTTGTAAA
    GACGCGGGTG GAAAGAAGTC GTCCTCTGCC AAACCGTGCC AGCGTGTGCT GCAGGACCAC
    GAGTGTGTGT TTCAGGCTCA GAGCCGCTGG AAGGGGGCTG GAAAATTCAT CCTCAAACTC
    AAAGAGCAGT TGGCTCGTGA GGACAAGCGT AAAGGTGTGT CGTTCGCCAG TGAGCTGAGG
    AAGCTGACGG GTCGCAGTCG CAGTGTGACG GTCAACACAA ACAGCAACGA CACTCACAGT
    AAGGAAGAGA AGAGCGCATG TCCCAGCATG AGCTGTGTGC CGGAGACATC GCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304703.1
    CDS419..7135
    Translation

    Target ORF information:

    RefSeq Version XM_009306428.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio phospholipase C, epsilon 1 (plce1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009306428.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGAGGCTTC ATGTTGAGAG ACAAGCGCAT ATTGGCATCA AGAGCTCCAT GGCCTCCAAT 
    AGGGCTTCGC CTGTACTGGG AGTGAAGAGT CTTGGTAAGG AGACTTCAAG TAATGGAGAT
    GCTCGTTTAT TAATGAACTA CAGTTTGCCT GGGGGCACAC CCCCTCAGAC ACCAGTACAT
    TTGGAGGGGG ACTCAGTGGG CACAGTGTGC AGAATATGGA AGTCTTTGGA TATCAGGTCT
    AAGAGTTTGG ATTGCTTAGA TGAGACCTTC AGGTCCAAAA ACTGCAGCTG TGAGGCTATA
    AGGCACAGGG AAGAAGGATC CTACAATTTC CATATGTGTG GGTTTTGTGA TTCAGTGGAA
    AATGCAGAAG GCTGCCCGAA GAATACTTTT TCTCCTAGAA GCTGGGGAAC GGTTCCCCAA
    AAGAGGAAAG CAGAAGCTTT TTCTTCTGGT ATTACTTCCC TTGATGAACC TCGACCAACA
    TTGAGTGGCT CAGATGGGGA ACTGTGCCCA AAGGAATTAG TCATCAGCCA AAACATGGAG
    AACCTGCCTT TCTGTGGGTT GGATTTGAGG AGAAGCAGTG TGCCAGTATC CACACCACAA
    GGCCCTCAAT GCGCCAACGG TTGCGGCACT GCCGAGAATA ATAAGATGCG AAACAGACTG
    GACTTTTTGT GTGCCGTGTG CCATAAAAAT GGATTCTCTA GCCTAGAAAG GCTTAACAGA
    AACACCAAAC CCATGTGCTC TAAAGGTAAG CCCCTTAAGA GGTCCAAACC CTTCAGGTTC
    TCTGTACCTG ACAGTAGTGA TGGCGAGGGC ACCGTATCCG ACAGCGAGTT TGTGCGTAAC
    AGGAAAGATC GATCTACTGT GCTGGTCAGG AGATATCTCA AGAACAACCA GAAATTTAAA
    AAGACCGTGT GCACAGGCAC TAAAGCTATA GTGAAGACTC TACCCACCGG CTGCATCTCT
    GACAGGATCT GGGAGTCTGT GTGCAATGAG AGGAGCAGGC ATGAGGCGAG CGGACACAGC
    AAAGCGCTTC AGGCCATCCA GCTGCAGGTT TCCAGGGCCC TTCTCGCCTA TCCCGGGGCC
    AGGCTTGCAG AGATTGATCA TGCTGCTCCT ACTTTGAGTC CTGAAGTCAG TCTCCTGCAC
    AGATCCAGGA AGCTGCTGGC CCAGGCGGTG TCATGGCCGC CGGTCTCACC GTCGCAGATT
    AGATTTCGGG CTTGTCATCG CAGAATCAGC ATTGCAGCCC ATCTGAGCGG GGCCCTGCTG
    GAGGCCACAG CTGCTCTTGG GGCCCGCACT TCCCTGCCCA GCTTCACTGT TGGCTCCACA
    GGACGAGCAA TGCTGAAAGA ACGGCAGCTG TGCAGCTCCA TGGCCAACTC CAGCATCCTG
    CCATCTGCTG TGACGGGGAT CAGTAAGGAA CTAGCTGACC TGCGCCACCT CATCCAGTTT
    CCAGAGGAAA TCGCCACCAT CCTGACAGAA CAGGAGCAGC AGCTATACCG CCGCGTCTTC
    CCCCTCGACT ACCTCAGCTT TCTCACCCGA GACCTGGGCA GCCCAGAGTG CCACAAACGC
    CACCCGCATC TAAAAGCTTC CCTGTCAGCC CCCATCATGC CCACTCAGAA TGACAACCAC
    AACACTGTTG AAGACCTCGT CACAAGATTC AATGAGGTGA GCTCATGGGT GACCTGGCTT
    ATCCTGACGG CGGGCTCCAT GGAGGAGAAG AGGGAGGTTT TCTCGTACCT CGTCCATGTG
    GCCAAGTGCT CGTGGAACAT GGGCAACTAC AACGCGGTCA TGGAGTTCTT GGCAGGCCTC
    AGGTCCCGCA AAGTGCTGAA GATGTGGCAG TTTATGGATC AGGCAGATAT TGAGACCATG
    CGAGGTCTAA AGGATGCCAT GGCCCAGCAC GAATCATCAT CTGAGTACAA GAAAGTCGTC
    AGCCGTGCCC TCAATATCCC AGGATGCAAA GTGGTGCCTT TCTGTGGCGT GTTCCTCAAG
    GAGCTGAGTG AGGCTCTGGA TGGCGCGGCC AGCATTATTG GACTGCGTCC GTCTTTTGAT
    TCTCAAGAAG ATCCTGTTGA GTTTGTCACG GACTACAACG GCCAGCAGCA CTTTCTGCAG
    AGGCTTGGAA GTGATGGACT TCACAGCTCA GATAAAGAGG CGACCGTCAG TAACATACTG
    CAGACCATCC GCAGCTGCAA CCGAAGCCTG GAGGCTGAGG AGCCTGAGGA GAGGGCACGA
    GAGATCACCG TTTGCCCCAA AAATTCCTTT AAAGACAAGA GCAGAAACCA GTTTTCAATC
    GGCGACCTCT CAGACTCTGA AGGGGACCCA CTTGAGACAG TTAAGGATGT TGTAGACCTC
    CAGACCACCG AAGATGTTCG CGGACCCTTT AGCCATGGGA CTGAGCTCAT TCCCTGGTAC
    GTTCTGTCGC TTCAGCCGGA TATCCATCAG TTCCTGCTCC AAGGAGCAAC CGTCATCCAC
    TACGACCCCG AGAGCCACCT CACCGCCCGC TGTCTTCTCC GTCTACAGCC CGATAACTGC
    TTCCTCACCT GGTGCAAACC CCACAGTAGC TGCAGCCTAT ATGGAAGAGC TAGAACCTTC
    ATGGGACATC CAGCATCCCC AGACCACTTA CACATCGGCC AACCTGTTCA CTGCGGACTC
    TCTGACGGGC TCCTGGATCT TAATGTGGTG AAGGCTGTGT TTATGGGTCA CCCTGGAGTG
    GACGTCAACT ATGTCTGCCT GCAGCACAAA CTGTGTAACA TGAATCCTGG TGAAAATGGT
    GTCACGCTGC TATATGGACT TCACACCACA GACAACAGGC TCCTGCACTT TGTGGCCCCG
    AAACACACGG CTCGCATGCT GCACGAGGGC CTCCAGGAGC TGCTGAACTC TATTAGGAAG
    ATCAGGAAAT TCCCTGACCA GAGACTGCAA TGGCTGAGAA AGCAATATGT TAGTTTGTAC
    CAGGAGGATG GTAGATTTGA AGGACCTACA CTGGCTCATG CTATCGAGCT GTTCGGAGGG
    AGACGCTGGA ATATGGGAAC TAGCGGCCCT GGATCCGCCA GCAGAGGTGC AGAGAAGAAC
    TCGGCTCAGA AGAACAGTCC ACTGGGCATC AACTCCAATG TCAAAAAAAA GAAAAAGGCC
    TTAGTCAGGG GGGACAGTGG CGATGGAACT GATGATGAGA TGACCGCACG CAAGACCAGG
    AGTTGCAAAG AGACTTTGGG GAGGAGAGAG TCAGATGCTC TGGAAAATGT TGAGCAGGAA
    GAGGCTGAGG ACTGCAGTAC CTTTAGCCAA CCTGGACCAT TTTCCTTATC CAACAACAAA
    GGCCAGTCTC CGGGATCCTC CTCGTCCTCC TCCACCTCTT CTTCTGTTGC CTCCTTTTGC
    ACAAACTCCT CCACCCCTAC ACGTCCACAT TCCTCCCCAA TCCTCCCCAC AAACCTCAAG
    AGTCAGCCAG GGGCCTGGAG CAGTCGAAGC TGGCACGGTC GAGGGAAAGG CTGCTTCAGA
    GGTTTCCAGG ACCTCATGAT CTCCGACAGC ATCATGAGCT TTGTCGAATT TGTGGAGCTC
    TTCAAATCTT TTAGCATTCG CAGTCGTAAG GATCTGAAGG AACTATTCGA CACCTACGCC
    GTGCCCTGCA GTCGCTCTGG CCCTGAGTCT GTCCCCCTCT ACACCACACT TAGGATTGAC
    GACAAGCTAA CCGGACTGCA GCCAGATCTG GATCTCCTCA CTCGCAACGG CTCTGACCTG
    GGCCTTTTCA TTCGCACCCG CCAGCAAATG TCTGAAAACC AGAAGCAGAT TTCAGACGCC
    ATCGCAGCTG CCAGTATTGT GACCAATGGC ACTGGAGTGG AGAATTCATC GCTGGGTGTG
    CTGGGACTGG CCATCTCGCA GCTCAATGAT TTCTTGGTCA ACTGTCAGAG AGAACATCTG
    AGCTATGATG AGATCCTCAG CATCATACAG AAATTTGAGC CTTCTTCAAA CATGCGTCAG
    ATGGGGTGGA TGTCGTTTGA AGGATTCGCC AGGTTTCTAA TGGACAAGGA CAACTTTGCC
    TCCAAAAATG AGGAGTCTCA AGTGAACCTG GATGAGCTCC AGCATCCGCT GTCTTACTAT
    TACATCGAGT CCTCTCATAA CACATACCTG ACGGGGCATC AGCTGAAGGG AGAGTCTTCA
    GTCGAGCTCT ACAGCCAGGT GTTGCTGCAG GGTTGTCGCA GCGTTGAGTT GGACTGCTGG
    GATGGGGATG ACGGCATGCC TGTTATTTAC CATGGACATA CTCTTACCAC TAAGATTCCT
    TTCAAGGATG TTGTAGAGGC CATCAACCGT GCAGCATTTG TGAACTCAGA GATGCCTGTG
    ATTCTGTCCA TTGAAAACCA CTGCTCCTTA CCACAGCAGC GCAAAATGGC TGAGATTTTT
    AAGATGGTGT TTGGTGAGAA GCTGGTGACC AAGTTTCTGT TTGAAAGTGA TTTCGCGGAC
    GAGCCTTTGC TTCCGTCTCC CCTTCAGCTC CGGGGGAAAA TCCTCCTGAA GAATAAAAAG
    TTAAAAGCTC ACCAGGCTCC TGTTGACATT CTCAAACAAA AGGCGCATCA GCTTGCCCAC
    ATGCAGGCTC AGGCCAACAA CGGCACAGTC AGCACAACGC CACTGGGAAA CAATGATGAA
    GAGGAGGAGG AGGAAGACGA GTACGATTAC GATTACGAGT CGCTGTCTGA CGACAATATT
    CTGGATGACA AACCTGAAGG CAAGTCATCT ACGGAGAAAC TTCAGTACGA GTCCAACGAT
    GAGATGCCAA AGCGGTTTAA AAAAGCTGGA AGCAAAGGAA AGATGTTTGA CATGGAGCTG
    GGAGAGGAGT TTTACCTGCC CCAGAACGAG AAGGAGAGTA GACAGATTGC TCAAGAGCTG
    TCAGATCTGG TCATCTACTG CCAGGCTATC AAATTTCCAG GCCTCTCCAC CCTCTCTCCA
    TCAGGCTCTG GAAGGGGGAA AGACAGAAAG AGCCGGAAAT CCATCTTTGG CAGCGCTCCT
    GCTCGCGGGA GCCCCGGGGA GCCTGTGACG CTGGTCCGAG CTCCAGGGAA AGCCAGTCTT
    GAGGGCATTC GCATGAACTC GGAGGATCAA TTGTGTCTGA GCCCGAGCAC CTCTCTGAGC
    TCCATCATCC GCACGCCCAA ATGCTACCAC ATCTCCTCTG TGAACGAGAA TGCAGCGAAG
    CGCTTGTGTC GCCGCTACTC GCAGAAGCTG ATCCAGCACA CCAGCTGCCA GCTGCTGCGC
    ACGTACCCAG CGGCCACACG CATTGACTCT GCCAACCCCA ATCCGCTCAT CTTCTGGCTG
    CACGGAGTCC AGCTGGTGGC CCTCAACTAC CAGACTGATG ATCTGCCGAT GCAGCTGAAC
    GCGGCTCTAT TCGAGGCTAA TGGTCACTGT GGTTTTGTGC TGAAGCCTCC AGTCCTGTGG
    GACAGAAGCT GTCCTCTCTA TCAGCACTTT TACCCTCTGG ACCGAGACCT GGAGAACATG
    ACCCCAACGC TATACACACT CACTATTGTG TCGGGGCAGA ACGTGTGTCC AGGAAACTCA
    AACGGCAGTC CGTGTGTCGA AGTGGAGGTG TTGGGAATGC CGGCGGACAG CTGTCATTTC
    CGAACTAAAC CCATACACCG CAACACACTC AATCCCATGT GGAACGAACA CTTCCAGTTC
    CACGTGCACT TTGAAGACCT GGCGTTCCTG CGCATCGCTG TGGTGGAGAA CAACAGCTCT
    CAGGTCACCG CTCAGAGGAT ACTTCCCCTC AAAACACTCA GAGCAGGCTA CCGGCACCTG
    CAGCTGAGGA ACCTGCACAA CGAGCCTCTG GAGGTGTCCA GTCTGTTCAT GTTCAGCCGC
    AGGACGGAGG AGAGCCCGAC CGGAGGACCA CCGTCTGCGT CTGTGCTGTT CAGCACCGAG
    GAGAGACGAT CTGTGCAGCA GCACAAGGTG ACCGTGCATG GAGTCCCCGG ACCTGAACCC
    TTCACTGTGA TCTGTGTCGA TGAATTCACC ACAGCCAAAC AGCTCCTGGA CTCTCTGTTT
    CCGACTGCCT CTTTTAAGTA TATGCTGATG GAGGAGAGAG TGTCTCTGTC GAAGGAGAAG
    AAGGCTCCTC AGCAGCGTCC TCTAGCCAAT GATGAGAGAC TGCTGAAGCT CATTCACAGC
    TGGCAGCCTG AGGACGGATA TGTGGGCAGA ATATACCTCA AAACCAGAGA ACAGAACTGC
    AGTGAGAAGA CGAGCGTCCC GCTGGAGTCA CTGGAGGAGC TCGGCAGCTT AGAGGACGAC
    ACCTTCTTCG TGCAGGTCCA TGATGTTTCT CCAGAACAGC CTCACACCGT CATCAAAGCC
    CCGCGATACA GCACAGCACA GGACATCATC CAGCAGACTC TGAGCAAAGC CAAGTATTCT
    CTGAGTATCC TGAGCAACCC CAACCCATGT GACTACGTCC TGATGGAGGA GCTTTGTAAA
    GACGCGGGTG GAAAGAAGTC GTCCTCTGCC AAACCGTGCC AGCGTGTGCT GCAGGACCAC
    GAGTGTGTGT TTCAGGCTCA GAGCCGCTGG AAGGGGGCTG GAAAATTCAT CCTCAAACTC
    AAAGAGCAGT TGGCTCGTGA GGACAAGCGT AAAGGTGTGT CGTTCGCCAG TGAGCTGAGG
    AAGCTGACGG GTCGCAGTCG CAGTGTGACG GTCAACACAA ACAGCAACGA CACTCACAGT
    AAGGAAGAGA AGAGCGCATG TCCCAGCATG AGCTGTGTGC CGGAGACATC GCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.