PLCE1 cDNA ORF clone, Bos taurus(cattle)

The following PLCE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa230874 XM_024985630.1
Latest version!
Bos taurus phospholipase C epsilon 1 (PLCE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa01621 NM_001205852.1
Latest version!
Bos taurus phospholipase C, epsilon 1 (PLCE1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa33573 NM_001205852.2
Latest version!
Bos taurus phospholipase C epsilon 1 (PLCE1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa01621 XM_005225543.4
Latest version!
Bos taurus phospholipase C epsilon 1 (PLCE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa33573 XM_005225543.3
Latest version!
Bos taurus phospholipase C epsilon 1 (PLCE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa230874
    Clone ID Related Accession (Same CDS sequence) XM_024985630.1
    Accession Version XM_024985630.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6762bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGACTTCTG AAGAAATGGC AGGTTCTGTT CTCAGACCTG TGAGTCAGAG AAAAGTGATC 
    TCTGCTCAGT CAACTGTAGA TGAAAGTAAT GATAAGGTCT CGGGCATCAG TGTTCCGAAG
    TCTCATCCTG TCAGGCAGAG TGGGGAGACT TCTCATACCA TCTCGCAACC GACCAAACTT
    ACAGAAGAAT CTTCTGGAAG CAACGCGCCC CAGATTTTCT CAGTAGCAAG GGAGAAAATA
    ACCAGTGACG AGAACAGTAA TGAAAAGTGC TGGGAGAAAA GCATGCCAGA TTCTGTGAAA
    AACCTTAACA TTAACTGCAA CAGCATATTG AAAAACCATC AGCACGGCCC TCCTCAGAGC
    CAGTTTTATG AAACGTGCAA TTCTATCACA GAGGAAGGTC TGTGTTTGGA AACTGGAATC
    CCTTCTTCAC TGGAAGGAAA GGTGTTCCCT GGAATTCAAC TGGAAATAGA CACATCTCCC
    ATGGGCATGA GTCCATTAGG AACTCAGTCG GCCATCATAG AGACAAGCAG GGCACACCCT
    GAAAGCAACA TGGCAGTGTT TCACTTGCGT TACGAAGTTG ACAGAAGAAT GTCAGACAGT
    TTCTGTCCCC TGTCAGATAA CTTGATTTTG GATGATTGTG GAAACTGTGT ACTACCTGCC
    GTTGGTGAGG AACAAGAGAA AAATTACATG GCATATACTT GCAAACTGAT GGAATTGACA
    AATAACTGTG ACAATAAGAA TAGGCAGCTG CAGTGTGATC ATTGTGACCC CTTGAATGAC
    AAATACTTGT GCTTTGAAGG CTCTTGCCCA AAGGCCGATG TGGTATGTTC AAGTGACAGC
    TTTTGCAGGG AGGATTTTAC TGACAGTCCA CCTGCCAAGA CCTTTCTGAG CCATTTTGAG
    GACTTCCCCG ATAATTGTGA AGAGGTAGAA GAAGATTTGT TCAAAAATAA AAAGGAGCGG
    TCTGCTTGGT TAGTGAGGAG ATTTTGCAAA AATGACAAGG AAGTTAAGAA ATCTGTGTTT
    ACTGGGACAA GGGCAATTGT GAGAACTTTG CCTTCTGGTC ACATTGGACT GGAGGCTTTG
    AGTTACATTG ATCAGAAGAA AAATGGTCTC TTATTGCCTC CTGGGAGAGT CATGGAACGG
    CTGTCAACAA TGGTGGTTAG GCAAGATGGT TCCCAAGGTC TGTCAGAAGC CCAGTGGTAT
    CCGATCTACA GTGCAGTGAG AAGGGGAGAA GCCACAGAAC AGACAATTAG ATCTCTTCTC
    CATTTCTTCG CCAAGCTCCC AGCCTCCGAG ACAGCCCATG AGAGGATCAG TGTCGGTCCG
    TACTTAAAGC AATGTGTCCG AGACACAGTG TGTGAGTATC GTGCCACCTT GCAAAGGACT
    TCCATATCTC AGTATATCAC CGGCTCTCTC CTGGAAGCCA CCACATCTTT AGGAGCAAGA
    AGCAGCCTCC TCAATTCTTT TGGAGGATCC ACGGGACGAA TGATGCTGAA AGAACGCCAA
    CCAGGCACCT CTATGGCCAA CTCCAGTGCC CTTCCTTCAA GTTCAGCTGG GATCAGCAAG
    GAACTGATTG ATCTGAAGCC CCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG
    GAGCAGGAGC AAAATATTTA CCGAAGGGTC TTGCCAGTTG ACTATCTTTT CTTCTTGACC
    CGGGGCTTGG GCACAACAGA ACGCCAGAGG TCCCTGCCCT GCCTCAAAGC ATCCATCTCA
    GCATCCATTC TTACCTCCCA GAAGGGAGAG CACAATGCCC TCGAAGATCT TGTGATGAGA
    TTTAATGAGG TGAGCTCCTG GGTGACGTGG CTGATCCTCG CGGCGGGCTC CATGGAAGAG
    AAGCGGGAAG TCTTCTCATA CTTGGTTCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC
    TACAATGCCG TCATGGAGTT CTTGGCCGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG
    CAGTTTATGG ACCAGTCTGA TCTGGAGACC ATGAGGAGCC TGAAGGACGC CATGGCCCAG
    CACGAGTCTT CCTGCGAGTA CAGGAAGGTG GTGACCCGAG CGCTGCACAT CCCCGGCTGC
    AAGGTGGTTC CCTTCTGCGG GGTGTTTCTG AAAGAGCTCT GTGAGGTGCT GGACGGAGCC
    TCTGGCCTCA TGAAGCTTTG CCCGAGGTAC AATTCGCAGG AAGAAACTCT GGAGTTTGTA
    GCCGATTATA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAGAATGG CTTAAAGAAT
    TCAGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGATCA TCAGGAGCTG CAGTCGAAGT
    CTGGAGACAG AGGAGGAGGA CAGCTCCAGT GAAGGGAGCA GCTCAAGGAA AAATTCCCTG
    AAGGATAAAG CCCGATGGCA GTTTATAATC GGAGATTTGT TGGATTCTGA AAATGACATC
    TTTGAGCAGC CCAAAGAGTG GGACTCTCAT GGATCGGAGG AGCCACAGAA AGCCTTTGAC
    CATGGGACAG AGCTCATCCC ATGGTATGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC
    CTGTTGCAGG GGGCCACGGT CATCCACTAT GATCAGGACA CGCACCTCTC TGCCCGCTGC
    TTCCTCCAGC TTCAGCCTGA CAATAGCACC TTGACCTGGA TAAAACCCAC CACTGCTTCC
    CCAGCCAGTG CTAAAGCAAA ACTTGGTGTG CTCAGTAACA CGTCTGAGCC TGGCAAATTC
    CCGTTACTGG GCAATGCTGG ATTAAGTGGC CTGGTGGAGG GGATCTTGGA TCTGTTTTCA
    GCAAAGGCTG TGTACATGGG ACACCCTAAC ATTGATATAC ACACTGTGTG TGTTCAGAAC
    AAACTGAGTA ACATGTCTCT CTCAGAGACT GGCGTGACAC TGCTCTACGG GCTGCAGACC
    ACGGACAATA GATTACTGCA CTTTGTGGCG CCAAAGCACA CAGCTAAAAT GCTCTTCAGT
    GGATTGTTGG AGCTCACGAG AGCTGTGAGG AAGATGAGGA AGTTCCCTGA CCAAAGACAG
    CATTGGCTGC GGAAACAGTA CGTCAGCCTC TACCAGGAGG ATGGACGATA TGAAGGCCCA
    ACTTTGGCTC AAGCTGTAGA GCTGTTTGGT GGCAGACGAT GGAGTACTCG AAACCCCAGC
    CCTGGGACAT CAGCAAAGAG CGCTGAGAAG CCCAACACCT TGAGTATAAG CAACACCAAG
    AAAAAGAAGA AAATCCTTAT GAGGGGTGAG AGTGGAGAGG TGACGGATGA TGAGATGGCC
    ACCCGCAAGG CCAGAACACA CAAAGAGAGC CGGAGCCGGA GTGGCTCTGA TCCTCAAGAT
    GTCAACGAAC AAGAAGAATC AGAGGCAAAT GCCATCATGA ACCCTCCCAA CCCTCTCCCT
    TCCAGAAGAG CCCACTCCTT GACCACGACT GGGCCCCCTA CCTTGGCTGC CGGGACAGCA
    TCTCCCATCA GGCCAGTGTC CTCTCCTGTG CTGCCTGCTT CCAACAGGAG CCCAACCAGT
    GCTTGGAACA GCAGCAGCGG CTGGCCAGGG AAGATCAAAG GAGGGATGCA GGGCTTTCAG
    AGCTTCATGG TTTCAGACAG CAGCATGAGT TTCGTGGAAT TTGTTGAGCT GTTCAAGTCA
    TTCAGTGTGA GGAGCCGCAA GGACCTGAAG GACCTCTTTG ACATCTACGC CGTACCCTGT
    GACCGAGCTG GCTCTGAGGC AGCCCCCCTC TATACCAACC TGACCATTGA CGAAAATATC
    AGCGGTCTTC AGCCTGACCT AGATTTGTTG ACCAGAAATG TCTCAGATTT GGGATTATTC
    ATCAAGACTA AACAGCAGCT GTCTGACAAC CAGAGGCAGA TATCTGATGC CATTGCTGCT
    GCCAGTATTG TGACAAATGG CACCGGGGTG GAGAGCACAT CTCTGGGTGT GTTTGGAGTT
    GGTATCCTCC AGCTCAATGA CTTCCTAGTG AATTGCCAAG GAGAACACTA TACTTATGAT
    GAAATCCTCA GCATTATCCA GAAGTTCGAG CCTAGCGTTA GTATGTGTCA TCAAGGCCTA
    ATGTCATTTG AAGGATTTGC AAGGTTTCTA ATGGATAAAG ATAATTTTGC CTCGAAAAAT
    GATGAGTCAC AGGAGAACGT TAAAGAAATG CAGCTGCCCC TCTCCTACTA TTACATTGAA
    TCTTCACACA ATACCTACCT CACGGGCCAT CAGCTCAAAG GAGAATCCTC AGTAGAACTC
    TACAGCCAGG TCCTCCTGCA AGGCTGTAGG AGTGTAGAGT TGGACTGCTG GGACGGAGAT
    GATGGAATGC CCATCATTTA TCATGGACAC ACCCTGACAA CCAAGATCCC CTTCAAGGAA
    GTGGTTGAAG CCATCGATCG CAGTGCCTTC ATCAACTCTG ACCTACCCAT CATCATATCG
    ATTGAGAACC ACTGCTCATT GCCTCAGCAA CGAAAAATGG CAGAAATTTT CAAGACTGTG
    TTTGGAGAAA AACTGGTGGC TAAATTCTTA TTTGAGAGTG ATTTCTCAGA TGATCCTATG
    CTCCCCTCAC CTGACCAACT CAGAAGGAAA GTGCTTCTCA AAAATAAGAA ATTAAAAGCC
    CATCAGACAC CGGTGGATAT CTTAAAGCAA AAGGCTCATC AGCTAGCATC CATGCAAGCT
    CAGGCTTATA ATGGTAGCAA TGCCAACTCC CCACCTGCTA ATAATGAGGA AGAGGAAGAT
    GAAGAGGATG AATATGATTA TGATTATGAA TCCCTCTCTG ATGACAACAT TCTAGAAGAC
    AGACCTGAAA ATAAATCATG TAATGACAAA CTTCAGTTTG AGTATAATGA AGAAATCCCC
    AAGAGGATAA AGAAAGCAGA TAACTCTGCT TGCAACAAAG GGAAGGTTTA TGACATGGAA
    CTGGGAGAAG AATTTTATCT TCCTCAGAAT AAAAAGGAAA GCAGACAGAT TGCACCAGAG
    CTTTCTGACC TTGTCATCTA TTGCCAAGCA GTAAAATTCC CAGGTTCCTG TGAAGGAATG
    CGACAGGCCT GGGAGGACTC TGCTTTCTCC GTCAACCCAA CCACGTCCCT CAGCGCTATC
    ATTAGAACTC CCAAATGCTA CCATATCTCC TCACTGAATG AAAATGCCGC CAAACGTCTG
    TGTCGCAGGT ATTCTCAGAA ACTCATCCAG CACACCGCCT GCCAGCTGCT GCGGACGTAC
    CCGGCTGCCA CCCGCATCGA CTCCTCCAAC CCCAACCCCC TCCTGTTCTG GCTCCATGGG
    ATACAGCTCG TGGCGCTCAA CTACCAGACA GATGATCTCT CTTTACATTT AAATGCCGCC
    ATGTTTGAGG CCAATGGTGG CTGCGGTTAT GTTCTGAAAC CCCCAGTTCT GTGGGACAAG
    AACTGTTCCA TGTATCAGAA GTTCTCCCCC TTGGAAAGAG ACCTGGACAG CATGGAGCCT
    GCCATCTATT CTTTAACTAT TGTCTCTGGG CAGAACGTGT GCCCGAGTAA CAGCACGGGG
    AGCCCATGTA TCGAAGTCGA TGTCCTGGGC ATGGCCCTGG ACAGCTGCCA CTTCCGCACG
    AAGCCCATCC ACCGCAATAC TCTGAACCCC ATGTGGAACG AACAGTTTCT TTTCCGGGTT
    CACTTCGAAG ATCTCGTATT TCTTCGTTTT GCTGTCGTGG AGAACAACAG CTCGGTGGTC
    ACTGCTCAGA GAATCATCTC CCTCAAGGCT CTAAAGCGAG GATATCGACA TCTTCAGCTG
    AGAAATCTCC ACAATGAAGT CTTGGAAATC TCTAGTTTAT TCATAAACAG CAGGAGGATG
    GAAGAAAATT CTTCCGGCAA TACTGTGCTG GCCTCTCTGA TGTTTAATAC GGAAGAAAGA
    AAATGTTTGC AGACTCACAG AGTCACAGTG CATGGCGTCC CAGGCCCAGA GCCCTTTACT
    GTTTTCTCTA TAAACGGAGG CACTAAGGCA AAGCAGCTTC TCCAACAAAT TCTCGTTATG
    GATCAAGATA CCAAACCTAT TGCCACAGAT TATTTTTTAA TGGAGGAAAA ATATTTCATA
    TCTAAAGAGA AGAATGAATG TAGGAAACAG CCGTTCCAGA GAGCCATTGG TCCAGAAGAA
    GAGATCGTGC AGATTTTAAG CAGCTGGTTT CCAGAAGAGG GCTATGTTGG CAGGATTGTC
    TTAAAAACCC AGCAGGAAAA CCTGGAAGAG AAGAGCATTG CACAAGATGA CAAGGAGATG
    CTCCTGAGCT CAGAGGAGGA CAGCTTCTTT GTCCAGGTGC ATGACGTTTC CCCAGAGCAA
    CCACGCACGG TCATCAAGGC GCCGCGTGTC AGCACCGCGC AGGATGTCAT TCAGCAGACC
    TTATGCAAAG CCAAATATTC CTATAGCATC CTGAGCAACC CCAACCCGAG TGACTATGTG
    CTTTTGGAAG AGGTGGTGAA AGACACCGCC AACAGGAAAT CTTCCACCCC AAAGGCCTCC
    CAGCGCGTCC TTTTGGATCA GGAGTGTGTG TTTCAAGCTC AAAGCAAGTG GAAAGGTGCA
    GGAAAATTCA TCCTTAAGCT AAAGGAACAG GTGCAGGCAT CTCGAGAAGA TAAAAAAAAA
    GGCATCTCTT TTGCAAGCGA ACTCAAGAAG CTCACCAAGA CTAAACAGCC CCGAGGACCC
    ACATCACCTT CTCAGGTCTT GGCCTCAGAA AACGTCCAAA ACAAGGAAGA GAAACCTGTG
    GGTGGCTTGC CTTCCAGTGA CACCATAGAC TACCGACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841398.1
    CDS1..6762
    Translation

    Target ORF information:

    RefSeq Version XM_024985630.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C epsilon 1 (PLCE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024985630.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGACTTCTG AAGAAATGGC AGGTTCTGTT CTCAGACCTG TGAGTCAGAG AAAAGTGATC 
    TCTGCTCAGT CAACTGTAGA TGAAAGTAAT GATAAGGTCT CGGGCATCAG TGTTCCGAAG
    TCTCATCCTG TCAGGCAGAG TGGGGAGACT TCTCATACCA TCTCGCAACC GACCAAACTT
    ACAGAAGAAT CTTCTGGAAG CAACGCGCCC CAGATTTTCT CAGTAGCAAG GGAGAAAATA
    ACCAGTGACG AGAACAGTAA TGAAAAGTGC TGGGAGAAAA GCATGCCAGA TTCTGTGAAA
    AACCTTAACA TTAACTGCAA CAGCATATTG AAAAACCATC AGCACGGCCC TCCTCAGAGC
    CAGTTTTATG AAACGTGCAA TTCTATCACA GAGGAAGGTC TGTGTTTGGA AACTGGAATC
    CCTTCTTCAC TGGAAGGAAA GGTGTTCCCT GGAATTCAAC TGGAAATAGA CACATCTCCC
    ATGGGCATGA GTCCATTAGG AACTCAGTCG GCCATCATAG AGACAAGCAG GGCACACCCT
    GAAAGCAACA TGGCAGTGTT TCACTTGCGT TACGAAGTTG ACAGAAGAAT GTCAGACAGT
    TTCTGTCCCC TGTCAGATAA CTTGATTTTG GATGATTGTG GAAACTGTGT ACTACCTGCC
    GTTGGTGAGG AACAAGAGAA AAATTACATG GCATATACTT GCAAACTGAT GGAATTGACA
    AATAACTGTG ACAATAAGAA TAGGCAGCTG CAGTGTGATC ATTGTGACCC CTTGAATGAC
    AAATACTTGT GCTTTGAAGG CTCTTGCCCA AAGGCCGATG TGGTATGTTC AAGTGACAGC
    TTTTGCAGGG AGGATTTTAC TGACAGTCCA CCTGCCAAGA CCTTTCTGAG CCATTTTGAG
    GACTTCCCCG ATAATTGTGA AGAGGTAGAA GAAGATTTGT TCAAAAATAA AAAGGAGCGG
    TCTGCTTGGT TAGTGAGGAG ATTTTGCAAA AATGACAAGG AAGTTAAGAA ATCTGTGTTT
    ACTGGGACAA GGGCAATTGT GAGAACTTTG CCTTCTGGTC ACATTGGACT GGAGGCTTTG
    AGTTACATTG ATCAGAAGAA AAATGGTCTC TTATTGCCTC CTGGGAGAGT CATGGAACGG
    CTGTCAACAA TGGTGGTTAG GCAAGATGGT TCCCAAGGTC TGTCAGAAGC CCAGTGGTAT
    CCGATCTACA GTGCAGTGAG AAGGGGAGAA GCCACAGAAC AGACAATTAG ATCTCTTCTC
    CATTTCTTCG CCAAGCTCCC AGCCTCCGAG ACAGCCCATG AGAGGATCAG TGTCGGTCCG
    TACTTAAAGC AATGTGTCCG AGACACAGTG TGTGAGTATC GTGCCACCTT GCAAAGGACT
    TCCATATCTC AGTATATCAC CGGCTCTCTC CTGGAAGCCA CCACATCTTT AGGAGCAAGA
    AGCAGCCTCC TCAATTCTTT TGGAGGATCC ACGGGACGAA TGATGCTGAA AGAACGCCAA
    CCAGGCACCT CTATGGCCAA CTCCAGTGCC CTTCCTTCAA GTTCAGCTGG GATCAGCAAG
    GAACTGATTG ATCTGAAGCC CCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG
    GAGCAGGAGC AAAATATTTA CCGAAGGGTC TTGCCAGTTG ACTATCTTTT CTTCTTGACC
    CGGGGCTTGG GCACAACAGA ACGCCAGAGG TCCCTGCCCT GCCTCAAAGC ATCCATCTCA
    GCATCCATTC TTACCTCCCA GAAGGGAGAG CACAATGCCC TCGAAGATCT TGTGATGAGA
    TTTAATGAGG TGAGCTCCTG GGTGACGTGG CTGATCCTCG CGGCGGGCTC CATGGAAGAG
    AAGCGGGAAG TCTTCTCATA CTTGGTTCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC
    TACAATGCCG TCATGGAGTT CTTGGCCGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG
    CAGTTTATGG ACCAGTCTGA TCTGGAGACC ATGAGGAGCC TGAAGGACGC CATGGCCCAG
    CACGAGTCTT CCTGCGAGTA CAGGAAGGTG GTGACCCGAG CGCTGCACAT CCCCGGCTGC
    AAGGTGGTTC CCTTCTGCGG GGTGTTTCTG AAAGAGCTCT GTGAGGTGCT GGACGGAGCC
    TCTGGCCTCA TGAAGCTTTG CCCGAGGTAC AATTCGCAGG AAGAAACTCT GGAGTTTGTA
    GCCGATTATA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAGAATGG CTTAAAGAAT
    TCAGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGATCA TCAGGAGCTG CAGTCGAAGT
    CTGGAGACAG AGGAGGAGGA CAGCTCCAGT GAAGGGAGCA GCTCAAGGAA AAATTCCCTG
    AAGGATAAAG CCCGATGGCA GTTTATAATC GGAGATTTGT TGGATTCTGA AAATGACATC
    TTTGAGCAGC CCAAAGAGTG GGACTCTCAT GGATCGGAGG AGCCACAGAA AGCCTTTGAC
    CATGGGACAG AGCTCATCCC ATGGTATGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC
    CTGTTGCAGG GGGCCACGGT CATCCACTAT GATCAGGACA CGCACCTCTC TGCCCGCTGC
    TTCCTCCAGC TTCAGCCTGA CAATAGCACC TTGACCTGGA TAAAACCCAC CACTGCTTCC
    CCAGCCAGTG CTAAAGCAAA ACTTGGTGTG CTCAGTAACA CGTCTGAGCC TGGCAAATTC
    CCGTTACTGG GCAATGCTGG ATTAAGTGGC CTGGTGGAGG GGATCTTGGA TCTGTTTTCA
    GCAAAGGCTG TGTACATGGG ACACCCTAAC ATTGATATAC ACACTGTGTG TGTTCAGAAC
    AAACTGAGTA ACATGTCTCT CTCAGAGACT GGCGTGACAC TGCTCTACGG GCTGCAGACC
    ACGGACAATA GATTACTGCA CTTTGTGGCG CCAAAGCACA CAGCTAAAAT GCTCTTCAGT
    GGATTGTTGG AGCTCACGAG AGCTGTGAGG AAGATGAGGA AGTTCCCTGA CCAAAGACAG
    CATTGGCTGC GGAAACAGTA CGTCAGCCTC TACCAGGAGG ATGGACGATA TGAAGGCCCA
    ACTTTGGCTC AAGCTGTAGA GCTGTTTGGT GGCAGACGAT GGAGTACTCG AAACCCCAGC
    CCTGGGACAT CAGCAAAGAG CGCTGAGAAG CCCAACACCT TGAGTATAAG CAACACCAAG
    AAAAAGAAGA AAATCCTTAT GAGGGGTGAG AGTGGAGAGG TGACGGATGA TGAGATGGCC
    ACCCGCAAGG CCAGAACACA CAAAGAGAGC CGGAGCCGGA GTGGCTCTGA TCCTCAAGAT
    GTCAACGAAC AAGAAGAATC AGAGGCAAAT GCCATCATGA ACCCTCCCAA CCCTCTCCCT
    TCCAGAAGAG CCCACTCCTT GACCACGACT GGGCCCCCTA CCTTGGCTGC CGGGACAGCA
    TCTCCCATCA GGCCAGTGTC CTCTCCTGTG CTGCCTGCTT CCAACAGGAG CCCAACCAGT
    GCTTGGAACA GCAGCAGCGG CTGGCCAGGG AAGATCAAAG GAGGGATGCA GGGCTTTCAG
    AGCTTCATGG TTTCAGACAG CAGCATGAGT TTCGTGGAAT TTGTTGAGCT GTTCAAGTCA
    TTCAGTGTGA GGAGCCGCAA GGACCTGAAG GACCTCTTTG ACATCTACGC CGTACCCTGT
    GACCGAGCTG GCTCTGAGGC AGCCCCCCTC TATACCAACC TGACCATTGA CGAAAATATC
    AGCGGTCTTC AGCCTGACCT AGATTTGTTG ACCAGAAATG TCTCAGATTT GGGATTATTC
    ATCAAGACTA AACAGCAGCT GTCTGACAAC CAGAGGCAGA TATCTGATGC CATTGCTGCT
    GCCAGTATTG TGACAAATGG CACCGGGGTG GAGAGCACAT CTCTGGGTGT GTTTGGAGTT
    GGTATCCTCC AGCTCAATGA CTTCCTAGTG AATTGCCAAG GAGAACACTA TACTTATGAT
    GAAATCCTCA GCATTATCCA GAAGTTCGAG CCTAGCGTTA GTATGTGTCA TCAAGGCCTA
    ATGTCATTTG AAGGATTTGC AAGGTTTCTA ATGGATAAAG ATAATTTTGC CTCGAAAAAT
    GATGAGTCAC AGGAGAACGT TAAAGAAATG CAGCTGCCCC TCTCCTACTA TTACATTGAA
    TCTTCACACA ATACCTACCT CACGGGCCAT CAGCTCAAAG GAGAATCCTC AGTAGAACTC
    TACAGCCAGG TCCTCCTGCA AGGCTGTAGG AGTGTAGAGT TGGACTGCTG GGACGGAGAT
    GATGGAATGC CCATCATTTA TCATGGACAC ACCCTGACAA CCAAGATCCC CTTCAAGGAA
    GTGGTTGAAG CCATCGATCG CAGTGCCTTC ATCAACTCTG ACCTACCCAT CATCATATCG
    ATTGAGAACC ACTGCTCATT GCCTCAGCAA CGAAAAATGG CAGAAATTTT CAAGACTGTG
    TTTGGAGAAA AACTGGTGGC TAAATTCTTA TTTGAGAGTG ATTTCTCAGA TGATCCTATG
    CTCCCCTCAC CTGACCAACT CAGAAGGAAA GTGCTTCTCA AAAATAAGAA ATTAAAAGCC
    CATCAGACAC CGGTGGATAT CTTAAAGCAA AAGGCTCATC AGCTAGCATC CATGCAAGCT
    CAGGCTTATA ATGGTAGCAA TGCCAACTCC CCACCTGCTA ATAATGAGGA AGAGGAAGAT
    GAAGAGGATG AATATGATTA TGATTATGAA TCCCTCTCTG ATGACAACAT TCTAGAAGAC
    AGACCTGAAA ATAAATCATG TAATGACAAA CTTCAGTTTG AGTATAATGA AGAAATCCCC
    AAGAGGATAA AGAAAGCAGA TAACTCTGCT TGCAACAAAG GGAAGGTTTA TGACATGGAA
    CTGGGAGAAG AATTTTATCT TCCTCAGAAT AAAAAGGAAA GCAGACAGAT TGCACCAGAG
    CTTTCTGACC TTGTCATCTA TTGCCAAGCA GTAAAATTCC CAGGTTCCTG TGAAGGAATG
    CGACAGGCCT GGGAGGACTC TGCTTTCTCC GTCAACCCAA CCACGTCCCT CAGCGCTATC
    ATTAGAACTC CCAAATGCTA CCATATCTCC TCACTGAATG AAAATGCCGC CAAACGTCTG
    TGTCGCAGGT ATTCTCAGAA ACTCATCCAG CACACCGCCT GCCAGCTGCT GCGGACGTAC
    CCGGCTGCCA CCCGCATCGA CTCCTCCAAC CCCAACCCCC TCCTGTTCTG GCTCCATGGG
    ATACAGCTCG TGGCGCTCAA CTACCAGACA GATGATCTCT CTTTACATTT AAATGCCGCC
    ATGTTTGAGG CCAATGGTGG CTGCGGTTAT GTTCTGAAAC CCCCAGTTCT GTGGGACAAG
    AACTGTTCCA TGTATCAGAA GTTCTCCCCC TTGGAAAGAG ACCTGGACAG CATGGAGCCT
    GCCATCTATT CTTTAACTAT TGTCTCTGGG CAGAACGTGT GCCCGAGTAA CAGCACGGGG
    AGCCCATGTA TCGAAGTCGA TGTCCTGGGC ATGGCCCTGG ACAGCTGCCA CTTCCGCACG
    AAGCCCATCC ACCGCAATAC TCTGAACCCC ATGTGGAACG AACAGTTTCT TTTCCGGGTT
    CACTTCGAAG ATCTCGTATT TCTTCGTTTT GCTGTCGTGG AGAACAACAG CTCGGTGGTC
    ACTGCTCAGA GAATCATCTC CCTCAAGGCT CTAAAGCGAG GATATCGACA TCTTCAGCTG
    AGAAATCTCC ACAATGAAGT CTTGGAAATC TCTAGTTTAT TCATAAACAG CAGGAGGATG
    GAAGAAAATT CTTCCGGCAA TACTGTGCTG GCCTCTCTGA TGTTTAATAC GGAAGAAAGA
    AAATGTTTGC AGACTCACAG AGTCACAGTG CATGGCGTCC CAGGCCCAGA GCCCTTTACT
    GTTTTCTCTA TAAACGGAGG CACTAAGGCA AAGCAGCTTC TCCAACAAAT TCTCGTTATG
    GATCAAGATA CCAAACCTAT TGCCACAGAT TATTTTTTAA TGGAGGAAAA ATATTTCATA
    TCTAAAGAGA AGAATGAATG TAGGAAACAG CCGTTCCAGA GAGCCATTGG TCCAGAAGAA
    GAGATCGTGC AGATTTTAAG CAGCTGGTTT CCAGAAGAGG GCTATGTTGG CAGGATTGTC
    TTAAAAACCC AGCAGGAAAA CCTGGAAGAG AAGAGCATTG CACAAGATGA CAAGGAGATG
    CTCCTGAGCT CAGAGGAGGA CAGCTTCTTT GTCCAGGTGC ATGACGTTTC CCCAGAGCAA
    CCACGCACGG TCATCAAGGC GCCGCGTGTC AGCACCGCGC AGGATGTCAT TCAGCAGACC
    TTATGCAAAG CCAAATATTC CTATAGCATC CTGAGCAACC CCAACCCGAG TGACTATGTG
    CTTTTGGAAG AGGTGGTGAA AGACACCGCC AACAGGAAAT CTTCCACCCC AAAGGCCTCC
    CAGCGCGTCC TTTTGGATCA GGAGTGTGTG TTTCAAGCTC AAAGCAAGTG GAAAGGTGCA
    GGAAAATTCA TCCTTAAGCT AAAGGAACAG GTGCAGGCAT CTCGAGAAGA TAAAAAAAAA
    GGCATCTCTT TTGCAAGCGA ACTCAAGAAG CTCACCAAGA CTAAACAGCC CCGAGGACCC
    ACATCACCTT CTCAGGTCTT GGCCTCAGAA AACGTCCAAA ACAAGGAAGA GAAACCTGTG
    GGTGGCTTGC CTTCCAGTGA CACCATAGAC TACCGACAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01621
    Clone ID Related Accession (Same CDS sequence) XM_005225543.4 , NM_001205852.1
    Accession Version NM_001205852.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2011-10-23
    Organism Bos taurus(Cattle)
    Product 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase epsilon-1
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03030155.1, AAFC03013767.1, AAFC03020409.1, AAFC03020417.1, AAFC03020410.1, AAFC03020416.1, AAFC03020413.1 and AAFC03038230.1. On Apr 20, 2011 this sequence version replaced XM_597245.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACTTCTG AAGAAATGGC AGGTTCTGTT CTCAGACCTG TGAGTCAGAG AAAAGTGATC 
    TCTGCTCAGT CAACTGTAGA TGAAAGTAAT GATAAGGTCT CGGGCATCAG TGTTCCGAAG
    TCTCATCCTG TCAGGCAGAG TGGGGAGACT TCTCATACCA TCTCGCAACC GACCAAACTT
    ACAGAAGAAT CTTCTGGAAG CAACGCGCCC CAGATTTTCT CAGTAGCAAG GGAGAAAATA
    ACCAGTGACG AGAACAGTAA TGAAAAGTGC TGGGAGAAAA GCATGCCAGA TTCTGTGAAA
    AACCTTAACA TTAACTGCAA CAGCATATTG AAAAACCATC AGCACGGCCC TCCTCAGAGC
    CAGTTTTATG AAACGTGCAA TTCTATCACA GAGGAAGGTC TGTGTTTGGA AACTGGAATC
    CCTTCTTCAC TGGAAGGAAA GGTGTTCCCT GGAATTCAAC TGGAAATAGA CACATCTCCC
    ATGGGCATGA GTCCATTAGG AACTCAGTCG GCCATCATAG AGACAAGCAG GGCACACCCT
    GAAAGCAACA TGGCAGTGTT TCACTTGCGT TACGAAGTTG ACAGAAGAAT GTCAGACAGT
    TTCTGTCCCC TGTCAGATAA CTTGATTTTG GATGATTGTG GAAACTGTGT ACTACCTGCC
    GTTGGTGAGG AACAAGAGAA AAATTACATG GCATATACTT GCAAACTGAT GGAATTGACA
    AATAACTGTG ACAATAAGAA TAGGCAGCTG CAGTGTGATC ATTGTGACCC CTTGAATGAC
    AAATACTTGT GCTTTGAAGG CTCTTGCCCA AAGGCCGATG TGGTATGTTC AAGTGACAGC
    TTTTGCAGGG AGGATTTTAC TGACAGTCCA CCTGCCAAGA CCTTTCTGAG CCATTTTGAG
    GACTTCCCCG ATAATTGTGA AGAGGTAGAA GAAGATTTGT TCAAAAATAA AAAGGAGCGG
    TCTGCTTGGT TAGTGAGGAG ATTTTGCAAA AATGACAAGG AAGTTAAGAA ATCTGTGTTT
    ACTGGGACAA GGGCAATTGT GAGAACTTTG CCTTCTGGTC ACATTGGACT GGAGGCTTTG
    AGTTACATTG ATCAGAAGAA AAATGGTCTC TTATTGCCTC CTGGGAGAGT CATGGAACGG
    CTGTCAACAA TGGTGGTTAG GCAAGATGGT TCCCAAGGTC TGTCAGAAGC CCAGTGGTAT
    CCGATCTACA GTGCAGTGAG AAGGGGAGAA GCCACAGAAC AGACAATTAG ATCTCTTCTC
    CATTTCTTCG CCAAGCTCCC AGCCTCCGAG ACAGCCCATG AGAGGATCAG TGTCGGTCCG
    TACTTAAAGC AATGTGTCCG AGACACAGTG TGTGAGTATC GTGCCACCTT GCAAAGGACT
    TCCATATCTC AGTATATCAC CGGCTCTCTC CTGGAAGCCA CCACATCTTT AGGAGCAAGA
    AGCAGCCTCC TCAATTCTTT TGGAGGATCC ACGGGACGAA TGATGCTGAA AGAACGCCAA
    CCAGGCACCT CTATGGCCAA CTCCAGTGCC CTTCCTTCAA GTTCAGCTGG GATCAGCAAG
    GAACTGATTG ATCTGAAGCC CCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG
    GAGCAGGAGC AAAATATTTA CCGAAGGGTC TTGCCAGTTG ACTATCTTTT CTTCTTGACC
    CGGGGCTTGG GCACAACAGA ACGCCAGAGG TCCCTGCCCT GCCTCAAAGC ATCCATCTCA
    GCATCCATTC TTACCTCCCA GAAGGGAGAG CACAATGCCC TCGAAGATCT TGTGATGAGA
    TTTAATGAGG TGAGCTCCTG GGTGACGTGG CTGATCCTCG CGGCGGGCTC CATGGAAGAG
    AAGCGGGAAG TCTTCTCATA CTTGGTTCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC
    TACAATGCCG TCATGGAGTT CTTGGCCGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG
    CAGTTTATGG ACCAGTCTGA TCTGGAGACC ATGAGGAGCC TGAAGGACGC CATGGCCCAG
    CACGAGTCTT CCTGCGAGTA CAGGAAGGTG GTGACCCGAG CGCTGCACAT CCCCGGCTGC
    AAGGTGGTTC CCTTCTGCGG GGTGTTTCTG AAAGAGCTCT GTGAGGTGCT GGACGGAGCC
    TCTGGCCTCA TGAAGCTTTG CCCGAGGTAC AATTCGCAGG AAGAAACTCT GGAGTTTGTA
    GCCGATTATA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAGAATGG CTTAAAGAAT
    TCAGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGATCA TCAGGAGCTG CAGTCGAAGT
    CTGGAGACAG AGGAGGAGGA CAGCTCCAGT GAAGGGAGCA GCTCAAGGAA AAATTCCCTG
    AAGGATAAAG CCCGATGGCA GTTTATAATC GGAGATTTGT TGGATTCTGA AAATGACATC
    TTTGAGCAGC CCAAAGAGTG GGACTCTCAT GGATCGGAGG AGCCACAGAA AGCCTTTGAC
    CATGGGACAG AGCTCATCCC ATGGTATGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC
    CTGTTGCAGG GGGCCACGGT CATCCACTAT GATCAGGACA CGCACCTCTC TGCCCGCTGC
    TTCCTCCAGC TTCAGCCTGA CAATAGCACC TTGACCTGGA TAAAACCCAC CACTGCTTCC
    CCAGCCAGTG CTAAAGCAAA ACTTGGTGTG CTCAGTAACA CGTCTGAGCC TGGCAAATTC
    CCGTTACTGG GCAATGCTGG ATTAAGTGGC CTGGTGGAGG GGATCTTGGA TCTGTTTTCA
    GCAAAGGCTG TGTACATGGG ACACCCTAAC ATTGATATAC ACACTGTGTG TGTTCAGAAC
    AAACTGAGTA ACATGTCTCT CTCAGAGACT GGCGTGACAC TGCTCTACGG GCTGCAGACC
    ACGGACAATA GATTACTGCA CTTTGTGGCG CCAAAGCACA CAGCTAAAAT GCTCTTCAGT
    GGATTGTTGG AGCTCACGAG AGCTGTGAGG AAGATGAGGA AGTTCCCTGA CCAAAGACAG
    CATTGGCTGC GGAAACAGTA CGTCAGCCTC TACCAGGAGG ATGGACGATA TGAAGGCCCA
    ACTTTGGCTC AAGCTGTAGA GCTGTTTGGT GGCAGACGAT GGAGTACTCG AAACCCCAGC
    CCTGGGACAT CAGCAAAGAG CGCTGAGAAG CCCAACACCT TGAGTATAAG CAACACCAAG
    AAAAAGAAGA AAATCCTTAT GAGGGGTGAG AGTGGAGAGG TGACGGATGA TGAGATGGCC
    ACCCGCAAGG CCAGAACACA CAAAGAGAGC CGGAGCCGGA GTGGCTCTGA TCCTCAAGAT
    GTCAACGAAC AAGAAGAATC AGAGGCAAAT GCCATCATGA ACCCTCCCAA CCCTCTCCCT
    TCCAGAAGAG CCCACTCCTT GACCACGACT GGGCCCCCTA CCTTGGCTGC CGGGACAGCA
    TCTCCCATCA GGCCAGTGTC CTCTCCTGTG CTGCCTGCTT CCAACAGGAG CCCAACCAGT
    GCTTGGAACA GCAGCAGCGG CTGGCCAGGG AAGATCAAAG GAGGGATGCA GGGCTTTCAG
    AGCTTCATGG TTTCAGACAG CAGCATGAGT TTCGTGGAAT TTGTTGAGCT GTTCAAGTCA
    TTCAGTGTGA GGAGCCGCAA GGACCTGAAG GACCTCTTTG ACATCTACGC CGTACCCTGT
    GACCGAGCTG GCTCTGAGGC AGCCCCCCTC TATACCAACC TGACCATTGA CGAAAATATC
    AGCGGTCTTC AGCCTGACCT AGATTTGTTG ACCAGAAATG TCTCAGATTT GGGATTATTC
    ATCAAGACTA AACAGCAGCT GTCTGACAAC CAGAGGCAGA TATCTGATGC CATTGCTGCT
    GCCAGTATTG TGACAAATGG CACCGGGGTG GAGAGCACAT CTCTGGGTGT GTTTGGAGTT
    GGTATCCTCC AGCTCAATGA CTTCCTAGTG AATTGCCAAG GAGAACACTA TACTTATGAT
    GAAATCCTCA GCATTATCCA GAAGTTCGAG CCTAGCGTTA GTATGTGTCA TCAAGGCCTA
    ATGTCATTTG AAGGATTTGC AAGGTTTCTA ATGGATAAAG ATAATTTTGC CTCGAAAAAT
    GATGAGTCAC AGGAGAACGT TAAAGAAATG CAGCTGCCCC TCTCCTACTA TTACATTGAA
    TCTTCACACA ATACCTACCT CACGGGCCAT CAGCTCAAAG GAGAATCCTC AGTAGAACTC
    TACAGCCAGG TCCTCCTGCA AGGCTGTAGG AGTGTAGAGT TGGACTGCTG GGACGGAGAT
    GATGGAATGC CCATCATTTA TCATGGACAC ACCCTGACAA CCAAGATCCC CTTCAAGGAA
    GTGGTTGAAG CCATCGATCG CAGTGCCTTC ATCAACTCTG ACCTACCCAT CATCATATCG
    ATTGAGAACC ACTGCTCATT GCCTCAGCAA CGAAAAATGG CAGAAATTTT CAAGACTGTG
    TTTGGAGAAA AACTGGTGGC TAAATTCTTA TTTGAGAGTG ATTTCTCAGA TGATCCTATG
    CTCCCCTCAC CTGACCAACT CAGAAGGAAA GTGCTTCTCA AAAATAAGAA ATTAAAAGCC
    CATCAGACAC CGGTGGATAT CTTAAAGCAA AAGGCTCATC AGCTAGCATC CATGCAAGCT
    CAGGCTTATA ATGGTAGCAA TGCCAACTCC CCACCTGCTA ATAATGAGGA AGAGGAAGAT
    GAAGAGGATG AATATGATTA TGATTATGAA TCCCTCTCTG ATGACAACAT TCTAGAAGAC
    AGACCTGAAA ATAAATCATG TAATGACAAA CTTCAGTTTG AGTATAATGA AGAAATCCCC
    AAGAGGATAA AGAAAGCAGA TAACTCTGCT TGCAACAAAG GGAAGGTTTA TGACATGGAA
    CTGGGAGAAG AATTTTATCT TCCTCAGAAT AAAAAGGAAA GCAGACAGAT TGCACCAGAG
    CTTTCTGACC TTGTCATCTA TTGCCAAGCA GTAAAATTCC CAGGCCTGTC AACTCTAAAT
    GCATCTGGCT CTAGCAGAGG AAAAGAAAGG AAAAGCAGGA AGTCCATTTT TGGCAACAAT
    CCGGGCAGAA TGAGCCCCGG GGAGACAGCA CCATTTAACA AAACATCTGG AAAAAGTTCC
    TGTGAAGGAA TGCGACAGGC CTGGGAGGAC TCTGCTTTCT CCGTCAACCC AACCACGTCC
    CTCAGCGCTA TCATTAGAAC TCCCAAATGC TACCATATCT CCTCACTGAA TGAAAATGCC
    GCCAAACGTC TGTGTCGCAG GTATTCTCAG AAACTCATCC AGCACACCGC CTGCCAGCTG
    CTGCGGACGT ACCCGGCTGC CACCCGCATC GACTCCTCCA ACCCCAACCC CCTCCTGTTC
    TGGCTCCATG GGATACAGCT CGTGGCGCTC AACTACCAGA CAGATGATCT CTCTTTACAT
    TTAAATGCCG CCATGTTTGA GGCCAATGGT GGCTGCGGTT ATGTTCTGAA ACCCCCAGTT
    CTGTGGGACA AGAACTGTTC CATGTATCAG AAGTTCTCCC CCTTGGAAAG AGACCTGGAC
    AGCATGGAGC CTGCCATCTA TTCTTTAACT ATTGTCTCTG GGCAGAACGT GTGCCCGAGT
    AACAGCACGG GGAGCCCATG TATCGAAGTC GATGTCCTGG GCATGGCCCT GGACAGCTGC
    CACTTCCGCA CGAAGCCCAT CCACCGCAAT ACTCTGAACC CCATGTGGAA CGAACAGTTT
    CTTTTCCGGG TTCACTTCGA AGATCTCGTA TTTCTTCGTT TTGCTGTCGT GGAGAACAAC
    AGCTCGGTGG TCACTGCTCA GAGAATCATC TCCCTCAAGG CTCTAAAGCG AGGATATCGA
    CATCTTCAGC TGAGAAATCT CCACAATGAA GTCTTGGAAA TCTCTAGTTT ATTCATAAAC
    AGCAGGAGGA TGGAAGAAAA TTCTTCCGGC AATACTGTGC TGGCCTCTCT GATGTTTAAT
    ACGGAAGAAA GAAAATGTTT GCAGACTCAC AGAGTCACAG TGCATGGCGT CCCAGGCCCA
    GAGCCCTTTA CTGTTTTCTC TATAAACGGA GGCACTAAGG CAAAGCAGCT TCTCCAACAA
    ATTCTCGTTA TGGATCAAGA TACCAAACCT ATTGCCACAG ATTATTTTTT AATGGAGGAA
    AAATATTTCA TATCTAAAGA GAAGAATGAA TGTAGGAAAC AGCCGTTCCA GAGAGCCATT
    GGTCCAGAAG AAGAGATCGT GCAGATTTTA AGCAGCTGGT TTCCAGAAGA GGGCTATGTT
    GGCAGGATTG TCTTAAAAAC CCAGCAGGAA AACCTGGAAG AGAAGAGCAT TGCACAAGAT
    GACAAGGAGA TGCTCCTGAG CTCAGAGGAG GACAGCTTCT TTGTCCAGGT GCATGACGTT
    TCCCCAGAGC AACCACGCAC GGTCATCAAG GCGCCGCGTG TCAGCACCGC GCAGGATGTC
    ATTCAGCAGA CCTTATGCAA AGCCAAATAT TCCTATAGCA TCCTGAGCAA CCCCAACCCG
    AGTGACTATG TGCTTTTGGA AGAGGTGGTG AAAGACACCG CCAACAGGAA ATCTTCCACC
    CCAAAGGCCT CCCAGCGCGT CCTTTTGGAT CAGGAGTGTG TGTTTCAAGC TCAAAGCAAG
    TGGAAAGGTG CAGGAAAATT CATCCTTAAG CTAAAGGAAC AGGTGCAGGC ATCTCGAGAA
    GATAAAAAAA AAGGCATCTC TTTTGCAAGC GAACTCAAGA AGCTCACCAA GACTAAACAG
    CCCCGAGGAC CCACATCACC TTCTCAGGTC TTGGCCTCAG AAAACGTCCA AAACAAGGAA
    GAGAAACCTG TGGGTGGCTT GCCTTCCAGT GACACCATAG ACTACCGACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192781.1
    CDS335..7228
    Translation

    Target ORF information:

    RefSeq Version NM_001205852.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus phospholipase C, epsilon 1 (PLCE1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205852.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACTTCTG AAGAAATGGC AGGTTCTGTT CTCAGACCTG TGAGTCAGAG AAAAGTGATC 
    TCTGCTCAGT CAACTGTAGA TGAAAGTAAT GATAAGGTCT CGGGCATCAG TGTTCCGAAG
    TCTCATCCTG TCAGGCAGAG TGGGGAGACT TCTCATACCA TCTCGCAACC GACCAAACTT
    ACAGAAGAAT CTTCTGGAAG CAACGCGCCC CAGATTTTCT CAGTAGCAAG GGAGAAAATA
    ACCAGTGACG AGAACAGTAA TGAAAAGTGC TGGGAGAAAA GCATGCCAGA TTCTGTGAAA
    AACCTTAACA TTAACTGCAA CAGCATATTG AAAAACCATC AGCACGGCCC TCCTCAGAGC
    CAGTTTTATG AAACGTGCAA TTCTATCACA GAGGAAGGTC TGTGTTTGGA AACTGGAATC
    CCTTCTTCAC TGGAAGGAAA GGTGTTCCCT GGAATTCAAC TGGAAATAGA CACATCTCCC
    ATGGGCATGA GTCCATTAGG AACTCAGTCG GCCATCATAG AGACAAGCAG GGCACACCCT
    GAAAGCAACA TGGCAGTGTT TCACTTGCGT TACGAAGTTG ACAGAAGAAT GTCAGACAGT
    TTCTGTCCCC TGTCAGATAA CTTGATTTTG GATGATTGTG GAAACTGTGT ACTACCTGCC
    GTTGGTGAGG AACAAGAGAA AAATTACATG GCATATACTT GCAAACTGAT GGAATTGACA
    AATAACTGTG ACAATAAGAA TAGGCAGCTG CAGTGTGATC ATTGTGACCC CTTGAATGAC
    AAATACTTGT GCTTTGAAGG CTCTTGCCCA AAGGCCGATG TGGTATGTTC AAGTGACAGC
    TTTTGCAGGG AGGATTTTAC TGACAGTCCA CCTGCCAAGA CCTTTCTGAG CCATTTTGAG
    GACTTCCCCG ATAATTGTGA AGAGGTAGAA GAAGATTTGT TCAAAAATAA AAAGGAGCGG
    TCTGCTTGGT TAGTGAGGAG ATTTTGCAAA AATGACAAGG AAGTTAAGAA ATCTGTGTTT
    ACTGGGACAA GGGCAATTGT GAGAACTTTG CCTTCTGGTC ACATTGGACT GGAGGCTTTG
    AGTTACATTG ATCAGAAGAA AAATGGTCTC TTATTGCCTC CTGGGAGAGT CATGGAACGG
    CTGTCAACAA TGGTGGTTAG GCAAGATGGT TCCCAAGGTC TGTCAGAAGC CCAGTGGTAT
    CCGATCTACA GTGCAGTGAG AAGGGGAGAA GCCACAGAAC AGACAATTAG ATCTCTTCTC
    CATTTCTTCG CCAAGCTCCC AGCCTCCGAG ACAGCCCATG AGAGGATCAG TGTCGGTCCG
    TACTTAAAGC AATGTGTCCG AGACACAGTG TGTGAGTATC GTGCCACCTT GCAAAGGACT
    TCCATATCTC AGTATATCAC CGGCTCTCTC CTGGAAGCCA CCACATCTTT AGGAGCAAGA
    AGCAGCCTCC TCAATTCTTT TGGAGGATCC ACGGGACGAA TGATGCTGAA AGAACGCCAA
    CCAGGCACCT CTATGGCCAA CTCCAGTGCC CTTCCTTCAA GTTCAGCTGG GATCAGCAAG
    GAACTGATTG ATCTGAAGCC CCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG
    GAGCAGGAGC AAAATATTTA CCGAAGGGTC TTGCCAGTTG ACTATCTTTT CTTCTTGACC
    CGGGGCTTGG GCACAACAGA ACGCCAGAGG TCCCTGCCCT GCCTCAAAGC ATCCATCTCA
    GCATCCATTC TTACCTCCCA GAAGGGAGAG CACAATGCCC TCGAAGATCT TGTGATGAGA
    TTTAATGAGG TGAGCTCCTG GGTGACGTGG CTGATCCTCG CGGCGGGCTC CATGGAAGAG
    AAGCGGGAAG TCTTCTCATA CTTGGTTCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC
    TACAATGCCG TCATGGAGTT CTTGGCCGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG
    CAGTTTATGG ACCAGTCTGA TCTGGAGACC ATGAGGAGCC TGAAGGACGC CATGGCCCAG
    CACGAGTCTT CCTGCGAGTA CAGGAAGGTG GTGACCCGAG CGCTGCACAT CCCCGGCTGC
    AAGGTGGTTC CCTTCTGCGG GGTGTTTCTG AAAGAGCTCT GTGAGGTGCT GGACGGAGCC
    TCTGGCCTCA TGAAGCTTTG CCCGAGGTAC AATTCGCAGG AAGAAACTCT GGAGTTTGTA
    GCCGATTATA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAGAATGG CTTAAAGAAT
    TCAGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGATCA TCAGGAGCTG CAGTCGAAGT
    CTGGAGACAG AGGAGGAGGA CAGCTCCAGT GAAGGGAGCA GCTCAAGGAA AAATTCCCTG
    AAGGATAAAG CCCGATGGCA GTTTATAATC GGAGATTTGT TGGATTCTGA AAATGACATC
    TTTGAGCAGC CCAAAGAGTG GGACTCTCAT GGATCGGAGG AGCCACAGAA AGCCTTTGAC
    CATGGGACAG AGCTCATCCC ATGGTATGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC
    CTGTTGCAGG GGGCCACGGT CATCCACTAT GATCAGGACA CGCACCTCTC TGCCCGCTGC
    TTCCTCCAGC TTCAGCCTGA CAATAGCACC TTGACCTGGA TAAAACCCAC CACTGCTTCC
    CCAGCCAGTG CTAAAGCAAA ACTTGGTGTG CTCAGTAACA CGTCTGAGCC TGGCAAATTC
    CCGTTACTGG GCAATGCTGG ATTAAGTGGC CTGGTGGAGG GGATCTTGGA TCTGTTTTCA
    GCAAAGGCTG TGTACATGGG ACACCCTAAC ATTGATATAC ACACTGTGTG TGTTCAGAAC
    AAACTGAGTA ACATGTCTCT CTCAGAGACT GGCGTGACAC TGCTCTACGG GCTGCAGACC
    ACGGACAATA GATTACTGCA CTTTGTGGCG CCAAAGCACA CAGCTAAAAT GCTCTTCAGT
    GGATTGTTGG AGCTCACGAG AGCTGTGAGG AAGATGAGGA AGTTCCCTGA CCAAAGACAG
    CATTGGCTGC GGAAACAGTA CGTCAGCCTC TACCAGGAGG ATGGACGATA TGAAGGCCCA
    ACTTTGGCTC AAGCTGTAGA GCTGTTTGGT GGCAGACGAT GGAGTACTCG AAACCCCAGC
    CCTGGGACAT CAGCAAAGAG CGCTGAGAAG CCCAACACCT TGAGTATAAG CAACACCAAG
    AAAAAGAAGA AAATCCTTAT GAGGGGTGAG AGTGGAGAGG TGACGGATGA TGAGATGGCC
    ACCCGCAAGG CCAGAACACA CAAAGAGAGC CGGAGCCGGA GTGGCTCTGA TCCTCAAGAT
    GTCAACGAAC AAGAAGAATC AGAGGCAAAT GCCATCATGA ACCCTCCCAA CCCTCTCCCT
    TCCAGAAGAG CCCACTCCTT GACCACGACT GGGCCCCCTA CCTTGGCTGC CGGGACAGCA
    TCTCCCATCA GGCCAGTGTC CTCTCCTGTG CTGCCTGCTT CCAACAGGAG CCCAACCAGT
    GCTTGGAACA GCAGCAGCGG CTGGCCAGGG AAGATCAAAG GAGGGATGCA GGGCTTTCAG
    AGCTTCATGG TTTCAGACAG CAGCATGAGT TTCGTGGAAT TTGTTGAGCT GTTCAAGTCA
    TTCAGTGTGA GGAGCCGCAA GGACCTGAAG GACCTCTTTG ACATCTACGC CGTACCCTGT
    GACCGAGCTG GCTCTGAGGC AGCCCCCCTC TATACCAACC TGACCATTGA CGAAAATATC
    AGCGGTCTTC AGCCTGACCT AGATTTGTTG ACCAGAAATG TCTCAGATTT GGGATTATTC
    ATCAAGACTA AACAGCAGCT GTCTGACAAC CAGAGGCAGA TATCTGATGC CATTGCTGCT
    GCCAGTATTG TGACAAATGG CACCGGGGTG GAGAGCACAT CTCTGGGTGT GTTTGGAGTT
    GGTATCCTCC AGCTCAATGA CTTCCTAGTG AATTGCCAAG GAGAACACTA TACTTATGAT
    GAAATCCTCA GCATTATCCA GAAGTTCGAG CCTAGCGTTA GTATGTGTCA TCAAGGCCTA
    ATGTCATTTG AAGGATTTGC AAGGTTTCTA ATGGATAAAG ATAATTTTGC CTCGAAAAAT
    GATGAGTCAC AGGAGAACGT TAAAGAAATG CAGCTGCCCC TCTCCTACTA TTACATTGAA
    TCTTCACACA ATACCTACCT CACGGGCCAT CAGCTCAAAG GAGAATCCTC AGTAGAACTC
    TACAGCCAGG TCCTCCTGCA AGGCTGTAGG AGTGTAGAGT TGGACTGCTG GGACGGAGAT
    GATGGAATGC CCATCATTTA TCATGGACAC ACCCTGACAA CCAAGATCCC CTTCAAGGAA
    GTGGTTGAAG CCATCGATCG CAGTGCCTTC ATCAACTCTG ACCTACCCAT CATCATATCG
    ATTGAGAACC ACTGCTCATT GCCTCAGCAA CGAAAAATGG CAGAAATTTT CAAGACTGTG
    TTTGGAGAAA AACTGGTGGC TAAATTCTTA TTTGAGAGTG ATTTCTCAGA TGATCCTATG
    CTCCCCTCAC CTGACCAACT CAGAAGGAAA GTGCTTCTCA AAAATAAGAA ATTAAAAGCC
    CATCAGACAC CGGTGGATAT CTTAAAGCAA AAGGCTCATC AGCTAGCATC CATGCAAGCT
    CAGGCTTATA ATGGTAGCAA TGCCAACTCC CCACCTGCTA ATAATGAGGA AGAGGAAGAT
    GAAGAGGATG AATATGATTA TGATTATGAA TCCCTCTCTG ATGACAACAT TCTAGAAGAC
    AGACCTGAAA ATAAATCATG TAATGACAAA CTTCAGTTTG AGTATAATGA AGAAATCCCC
    AAGAGGATAA AGAAAGCAGA TAACTCTGCT TGCAACAAAG GGAAGGTTTA TGACATGGAA
    CTGGGAGAAG AATTTTATCT TCCTCAGAAT AAAAAGGAAA GCAGACAGAT TGCACCAGAG
    CTTTCTGACC TTGTCATCTA TTGCCAAGCA GTAAAATTCC CAGGCCTGTC AACTCTAAAT
    GCATCTGGCT CTAGCAGAGG AAAAGAAAGG AAAAGCAGGA AGTCCATTTT TGGCAACAAT
    CCGGGCAGAA TGAGCCCCGG GGAGACAGCA CCATTTAACA AAACATCTGG AAAAAGTTCC
    TGTGAAGGAA TGCGACAGGC CTGGGAGGAC TCTGCTTTCT CCGTCAACCC AACCACGTCC
    CTCAGCGCTA TCATTAGAAC TCCCAAATGC TACCATATCT CCTCACTGAA TGAAAATGCC
    GCCAAACGTC TGTGTCGCAG GTATTCTCAG AAACTCATCC AGCACACCGC CTGCCAGCTG
    CTGCGGACGT ACCCGGCTGC CACCCGCATC GACTCCTCCA ACCCCAACCC CCTCCTGTTC
    TGGCTCCATG GGATACAGCT CGTGGCGCTC AACTACCAGA CAGATGATCT CTCTTTACAT
    TTAAATGCCG CCATGTTTGA GGCCAATGGT GGCTGCGGTT ATGTTCTGAA ACCCCCAGTT
    CTGTGGGACA AGAACTGTTC CATGTATCAG AAGTTCTCCC CCTTGGAAAG AGACCTGGAC
    AGCATGGAGC CTGCCATCTA TTCTTTAACT ATTGTCTCTG GGCAGAACGT GTGCCCGAGT
    AACAGCACGG GGAGCCCATG TATCGAAGTC GATGTCCTGG GCATGGCCCT GGACAGCTGC
    CACTTCCGCA CGAAGCCCAT CCACCGCAAT ACTCTGAACC CCATGTGGAA CGAACAGTTT
    CTTTTCCGGG TTCACTTCGA AGATCTCGTA TTTCTTCGTT TTGCTGTCGT GGAGAACAAC
    AGCTCGGTGG TCACTGCTCA GAGAATCATC TCCCTCAAGG CTCTAAAGCG AGGATATCGA
    CATCTTCAGC TGAGAAATCT CCACAATGAA GTCTTGGAAA TCTCTAGTTT ATTCATAAAC
    AGCAGGAGGA TGGAAGAAAA TTCTTCCGGC AATACTGTGC TGGCCTCTCT GATGTTTAAT
    ACGGAAGAAA GAAAATGTTT GCAGACTCAC AGAGTCACAG TGCATGGCGT CCCAGGCCCA
    GAGCCCTTTA CTGTTTTCTC TATAAACGGA GGCACTAAGG CAAAGCAGCT TCTCCAACAA
    ATTCTCGTTA TGGATCAAGA TACCAAACCT ATTGCCACAG ATTATTTTTT AATGGAGGAA
    AAATATTTCA TATCTAAAGA GAAGAATGAA TGTAGGAAAC AGCCGTTCCA GAGAGCCATT
    GGTCCAGAAG AAGAGATCGT GCAGATTTTA AGCAGCTGGT TTCCAGAAGA GGGCTATGTT
    GGCAGGATTG TCTTAAAAAC CCAGCAGGAA AACCTGGAAG AGAAGAGCAT TGCACAAGAT
    GACAAGGAGA TGCTCCTGAG CTCAGAGGAG GACAGCTTCT TTGTCCAGGT GCATGACGTT
    TCCCCAGAGC AACCACGCAC GGTCATCAAG GCGCCGCGTG TCAGCACCGC GCAGGATGTC
    ATTCAGCAGA CCTTATGCAA AGCCAAATAT TCCTATAGCA TCCTGAGCAA CCCCAACCCG
    AGTGACTATG TGCTTTTGGA AGAGGTGGTG AAAGACACCG CCAACAGGAA ATCTTCCACC
    CCAAAGGCCT CCCAGCGCGT CCTTTTGGAT CAGGAGTGTG TGTTTCAAGC TCAAAGCAAG
    TGGAAAGGTG CAGGAAAATT CATCCTTAAG CTAAAGGAAC AGGTGCAGGC ATCTCGAGAA
    GATAAAAAAA AAGGCATCTC TTTTGCAAGC GAACTCAAGA AGCTCACCAA GACTAAACAG
    CCCCGAGGAC CCACATCACC TTCTCAGGTC TTGGCCTCAG AAAACGTCCA AAACAAGGAA
    GAGAAACCTG TGGGTGGCTT GCCTTCCAGT GACACCATAG ACTACCGACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33573
    Clone ID Related Accession (Same CDS sequence) XM_005225543.3 , NM_001205852.2
    Accession Version NM_001205852.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02058845.1, DAAA02058846.1, DAAA02058847.1, DAAA02058848.1 and DAAA02058849.1. On Aug 13, 2017 this sequence version replaced NM_001205852.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145432, SAMN03145450 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACTTCTG AAGAAATGGC AGGTTCTGTT CTCAGACCTG TGAGTCAGAG AAAAGTGATC 
    TCTGCTCAGT CAACTGTAGA TGAAAGTAAT GATAAGGTCT CGGGCATCAG TGTTCCGAAG
    TCTCATCCTG TCAGGCAGAG TGGGGAGACT TCTCATACCA TCTCGCAACC GACCAAACTT
    ACAGAAGAAT CTTCTGGAAG CAACGCGCCC CAGATTTTCT CAGTAGCAAG GGAGAAAATA
    ACCAGTGACG AGAACAGTAA TGAAAAGTGC TGGGAGAAAA GCATGCCAGA TTCTGTGAAA
    AACCTTAACA TTAACTGCAA CAGCATATTG AAAAACCATC AGCACGGCCC TCCTCAGAGC
    CAGTTTTATG AAACGTGCAA TTCTATCACA GAGGAAGGTC TGTGTTTGGA AACTGGAATC
    CCTTCTTCAC TGGAAGGAAA GGTGTTCCCT GGAATTCAAC TGGAAATAGA CACATCTCCC
    ATGGGCATGA GTCCATTAGG AACTCAGTCG GCCATCATAG AGACAAGCAG GGCACACCCT
    GAAAGCAACA TGGCAGTGTT TCACTTGCGT TACGAAGTTG ACAGAAGAAT GTCAGACAGT
    TTCTGTCCCC TGTCAGATAA CTTGATTTTG GATGATTGTG GAAACTGTGT ACTACCTGCC
    GTTGGTGAGG AACAAGAGAA AAATTACATG GCATATACTT GCAAACTGAT GGAATTGACA
    AATAACTGTG ACAATAAGAA TAGGCAGCTG CAGTGTGATC ATTGTGACCC CTTGAATGAC
    AAATACTTGT GCTTTGAAGG CTCTTGCCCA AAGGCCGATG TGGTATGTTC AAGTGACAGC
    TTTTGCAGGG AGGATTTTAC TGACAGTCCA CCTGCCAAGA CCTTTCTGAG CCATTTTGAG
    GACTTCCCCG ATAATTGTGA AGAGGTAGAA GAAGATTTGT TCAAAAATAA AAAGGAGCGG
    TCTGCTTGGT TAGTGAGGAG ATTTTGCAAA AATGACAAGG AAGTTAAGAA ATCTGTGTTT
    ACTGGGACAA GGGCAATTGT GAGAACTTTG CCTTCTGGTC ACATTGGACT GGAGGCTTTG
    AGTTACATTG ATCAGAAGAA AAATGGTCTC TTATTGCCTC CTGGGAGAGT CATGGAACGG
    CTGTCAACAA TGGTGGTTAG GCAAGATGGT TCCCAAGGTC TGTCAGAAGC CCAGTGGTAT
    CCGATCTACA GTGCAGTGAG AAGGGGAGAA GCCACAGAAC AGACAATTAG ATCTCTTCTC
    CATTTCTTCG CCAAGCTCCC AGCCTCCGAG ACAGCCCATG AGAGGATCAG TGTCGGTCCG
    TACTTAAAGC AATGTGTCCG AGACACAGTG TGTGAGTATC GTGCCACCTT GCAAAGGACT
    TCCATATCTC AGTATATCAC CGGCTCTCTC CTGGAAGCCA CCACATCTTT AGGAGCAAGA
    AGCAGCCTCC TCAATTCTTT TGGAGGATCC ACGGGACGAA TGATGCTGAA AGAACGCCAA
    CCAGGCACCT CTATGGCCAA CTCCAGTGCC CTTCCTTCAA GTTCAGCTGG GATCAGCAAG
    GAACTGATTG ATCTGAAGCC CCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG
    GAGCAGGAGC AAAATATTTA CCGAAGGGTC TTGCCAGTTG ACTATCTTTT CTTCTTGACC
    CGGGGCTTGG GCACAACAGA ACGCCAGAGG TCCCTGCCCT GCCTCAAAGC ATCCATCTCA
    GCATCCATTC TTACCTCCCA GAAGGGAGAG CACAATGCCC TCGAAGATCT TGTGATGAGA
    TTTAATGAGG TGAGCTCCTG GGTGACGTGG CTGATCCTCG CGGCGGGCTC CATGGAAGAG
    AAGCGGGAAG TCTTCTCATA CTTGGTTCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC
    TACAATGCCG TCATGGAGTT CTTGGCCGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG
    CAGTTTATGG ACCAGTCTGA TCTGGAGACC ATGAGGAGCC TGAAGGACGC CATGGCCCAG
    CACGAGTCTT CCTGCGAGTA CAGGAAGGTG GTGACCCGAG CGCTGCACAT CCCCGGCTGC
    AAGGTGGTTC CCTTCTGCGG GGTGTTTCTG AAAGAGCTCT GTGAGGTGCT GGACGGAGCC
    TCTGGCCTCA TGAAGCTTTG CCCGAGGTAC AATTCGCAGG AAGAAACTCT GGAGTTTGTA
    GCCGATTATA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAGAATGG CTTAAAGAAT
    TCAGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGATCA TCAGGAGCTG CAGTCGAAGT
    CTGGAGACAG AGGAGGAGGA CAGCTCCAGT GAAGGGAGCA GCTCAAGGAA AAATTCCCTG
    AAGGATAAAG CCCGATGGCA GTTTATAATC GGAGATTTGT TGGATTCTGA AAATGACATC
    TTTGAGCAGC CCAAAGAGTG GGACTCTCAT GGATCGGAGG AGCCACAGAA AGCCTTTGAC
    CATGGGACAG AGCTCATCCC ATGGTATGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC
    CTGTTGCAGG GGGCCACGGT CATCCACTAT GATCAGGACA CGCACCTCTC TGCCCGCTGC
    TTCCTCCAGC TTCAGCCTGA CAATAGCACC TTGACCTGGA TAAAACCCAC CACTGCTTCC
    CCAGCCAGTG CTAAAGCAAA ACTTGGTGTG CTCAGTAACA CGTCTGAGCC TGGCAAATTC
    CCGTTACTGG GCAATGCTGG ATTAAGTGGC CTGGTGGAGG GGATCTTGGA TCTGTTTTCA
    GCAAAGGCTG TGTACATGGG ACACCCTAAC ATTGATATAC ACACTGTGTG TGTTCAGAAC
    AAACTGAGTA ACATGTCTCT CTCAGAGACT GGCGTGACAC TGCTCTACGG GCTGCAGACC
    ACGGACAATA GATTACTGCA CTTTGTGGCG CCAAAGCACA CAGCTAAAAT GCTCTTCAGT
    GGATTGTTGG AGCTCACGAG AGCTGTGAGG AAGATGAGGA AGTTCCCTGA CCAAAGACAG
    CATTGGCTGC GGAAACAGTA CGTCAGCCTC TACCAGGAGG ATGGACGATA TGAAGGCCCA
    ACTTTGGCTC AAGCTGTAGA GCTGTTTGGT GGCAGACGAT GGAGTACTCG AAACCCCAGC
    CCTGGGACAT CAGCAAAGAG CGCTGAGAAG CCCAACACCT TGAGTATAAG CAACACCAAG
    AAAAAGAAGA AAATCCTTAT GAGGGGTGAG AGTGGAGAGG TGACGGATGA TGAGATGGCC
    ACCCGCAAGG CCAGAACACA CAAAGAGAGC CGGAGCCGGA GTGGCTCTGA TCCTCAAGAT
    GTCAACGAAC AAGAAGAATC AGAGGCAAAT GCCATCATGA ACCCTCCCAA CCCTCTCCCT
    TCCAGAAGAG CCCACTCCTT GACCACGACT GGGCCCCCTA CCTTGGCTGC CGGGACAGCA
    TCTCCCATCA GGCCAGTGTC CTCTCCTGTG CTGCCTGCTT CCAACAGGAG CCCAACCAGT
    GCTTGGAACA GCAGCAGCGG CTGGCCAGGG AAGATCAAAG GAGGGATGCA GGGCTTTCAG
    AGCTTCATGG TTTCAGACAG CAGCATGAGT TTCGTGGAAT TTGTTGAGCT GTTCAAGTCA
    TTCAGTGTGA GGAGCCGCAA GGACCTGAAG GACCTCTTTG ACATCTACGC CGTACCCTGT
    GACCGAGCTG GCTCTGAGGC AGCCCCCCTC TATACCAACC TGACCATTGA CGAAAATATC
    AGCGGTCTTC AGCCTGACCT AGATTTGTTG ACCAGAAATG TCTCAGATTT GGGATTATTC
    ATCAAGAGTA AACAGCAGCT GTCTGACAAC CAGAGGCAGA TATCTGATGC CATTGCTGCT
    GCCAGTATTG TGACAAATGG CACCGGGGTG GAGAGCACAT CTCTGGGTGT GTTTGGAGTT
    GGTATCCTCC AGCTCAATGA CTTCCTAGTG AATTGCCAAG GAGAACACTA TACTTATGAT
    GAAATCCTCA GCATTATCCA GAAGTTCGAG CCTAGCGTTA GTATGTGTCA TCAAGGCCTA
    ATGTCATTTG AAGGATTTGC AAGGTTTCTA ATGGATAAAG ATAATTTTGC CTCGAAAAAT
    GATGAGTCAC AGGAGAACGT TAAAGAAATG CAGCTGCCCC TCTCCTACTA TTACATTGAA
    TCTTCACACA ATACCTACCT CACGGGCCAT CAGCTCAAAG GAGAATCCTC AGTAGAACTC
    TACAGCCAGG TCCTCCTGCA AGGCTGTAGG AGTGTAGAGT TGGACTGCTG GGACGGAGAT
    GATGGAATGC CCATCATTTA TCATGGACAC ACCCTGACAA CCAAGATCCC CTTCAAGGAA
    GTGGTTGAAG CCATCGATCG CAGTGCCTTC ATCAACTCTG ACCTACCCAT CATCATATCG
    ATTGAGAACC ACTGCTCATT GCCTCAGCAA CGAAAAATGG CAGAAATTTT CAAGACTGTG
    TTTGGAGAAA AACTGGTGGC TAAATTCTTA TTTGAGAGTG ATTTCTCAGA TGATCCTATG
    CTCCCCTCAC CTGACCAACT CAGAAGGAAA GTGCTTCTCA AAAATAAGAA ATTAAAAGCC
    CATCAGACAC CGGTGGATAT CTTAAAGCAA AAGGCTCATC AGCTAGCATC CATGCAAGCT
    CAGGCTTATA ATGGTAGCAA TGCCAACTCC CCACCTGCTA ATAATGAGGA AGAGGAAGAT
    GAAGAGGATG AATATGATTA TGATTATGAA TCCCTCTCTG ATGACAACAT TCTAGAAGAC
    AGACCTGAAA ATAAATCATG TAATGACAAA CTTCAGTTTG AGTATAATGA AGAAATCCCC
    AAGAGGATAA AGAAAGCAGA TAACTCTGCT TGCAACAAAG GGAAGGTTTA TGACATGGAA
    CTGGGAGAAG AATTTTATCT TCCTCAGAAT AAAAAGGAAA GCAGACAGAT TGCACCAGAG
    CTTTCTGACC TTGTCATCTA TTGCCAAGCA GTAAAATTCC CAGGCCTGTC AACTCTAAAT
    GCATCTGGCT CTAGCAGAGG AAAAGAAAGG AAAAGCAGGA AGTCCATTTT TGGCAACAAT
    CCGGGCAGAA TGAGCCCCGG GGAGACAGCA CCATTTAACA AAACATCTGG AAAAAGTTCC
    TGTGAAGGAA TGCGACAGGC CTGGGAGGAC TCTGCTTTCT CCGTCAACCC AACCACGTCC
    CTCAGCGCTA TCATTAGAAC TCCCAAATGC TACCATATCT CCTCACTGAA TGAAAATGCC
    GCCAAACGTC TGTGTCGCAG GTATTCTCAG AAACTCATCC AGCACACCGC CTGCCAGCTG
    CTGCGGACGT ACCCGGCTGC CACCCGCATC GACTCCTCCA ACCCCAACCC CCTCCTGTTC
    TGGCTCCATG GGATACAGCT CGTGGCGCTC AACTACCAGA CAGATGATCT CTCTTTACAT
    TTAAATGCCG CCATGTTTGA GGCCAATGGT GGCTGCGGTT ATGTTCTGAA ACCCCCAGTT
    CTGTGGGACA AGAACTGTTC CATGTATCAG AAGTTCTCCC CCTTGGAAAG AGACCTGGAC
    AGCATGGAGC CTGCCATCTA TTCTTTAACT ATTGTCTCTG GGCAGAACGT GTGCCCGAGT
    AACAGCACGG GGAGCCCATG TATCGAAGTC GATGTCCTGG GCATGGCCCT GGACAGCTGC
    CACTTCCGCA CGAAGCCCAT CCACCGCAAT ACTCTGAACC CCATGTGGAA CGAACAGTTT
    CTTTTCCGGG TTCACTTCGA AGATCTCGTA TTTCTTCGTT TTGCTGTCGT GGAGAACAAC
    AGCTCGGTGG TCACTGCTCA GAGAATCATC TCCCTCAAGG CTCTAAAGCG AGGATATCGA
    CATCTTCAGC TGAGAAATCT CCACAATGAA GTCTTGGAAA TCTCTAGTTT ATTCATAAAC
    AGCAGGAGGA TGGAAGAAAA TTCTTCCGGC AATACTGTGC TGGCCTCTCT GATGTTTAAT
    ACGGAAGAAA GAAAATGTTT GCAGACTCAC AGAGTCACAG TGCATGGCGT CCCAGGCCCA
    GAGCCCTTTA CTGTTTTCTC TATAAACGGA GGCACTAAGG CAAAGCAGCT TCTCCAACAA
    ATTCTCGTTA TGGATCAAGA TACCAAACCT ATTGCCACAG ATTATTTTTT AATGGAGGAA
    AAATATTTCA TATCTAAAGA GAAGAATGAA TGTAGGAAAC AGCCGTTCCA GAGAGCCATT
    GGTCCAGAAG AAGAGATCGT GCAGATTTTA AGCAGCTGGT TTCCAGAAGA GGGCTATGTT
    GGCAGGATTG TCTTAAAAAC CCAGCAGGAA AACCTGGAAG AGAAGAGCAT TGCACAAGAT
    GACAAGGAGA TGCTCCTGAG CTCAGAGGAG GACAGCTTCT TTGTCCAGGT GCATGACGTT
    TCCCCAGAGC AACCACGCAC GGTCATCAAG GCGCCGCGTG TCAGCACCGC GCAGGATGTC
    ATTCAGCAGA CCTTATGCAA AGCCAAATAT TCCTATAGCA TCCTGAGCAA CCCCAACCCG
    AGTGACTATG TGCTTTTGGA AGAGGTGGTG AAAGACACCG CCAACAGGAA ATCTTCCACC
    CCAAAGGCCT CCCAGCGCGT CCTTTTGGAT CAGGAGTGTG TGTTTCAAGC TCAAAGCAAG
    TGGAAAGGTG CAGGAAAATT CATCCTTAAG CTAAAGGAAC AGGTGCAGGC ATCTCGAGAA
    GATAAAAAAA AAGGCATCTC TTTTGCAAGC GAACTCAAGA AGCTCACCAA GACTAAACAG
    CCCCGAGGAC CCACATCACC TTCTCAGGTC TTGGCCTCAG AAAACGTCCA AAACAAGGAA
    GAGAAACCTG TGGGTGGCTT GCCTTCCAGT GACACCATAG ACTACCGACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192781.2
    CDS333..7226
    Translation

    Target ORF information:

    RefSeq Version NM_001205852.2
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C epsilon 1 (PLCE1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205852.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACTTCTG AAGAAATGGC AGGTTCTGTT CTCAGACCTG TGAGTCAGAG AAAAGTGATC 
    TCTGCTCAGT CAACTGTAGA TGAAAGTAAT GATAAGGTCT CGGGCATCAG TGTTCCGAAG
    TCTCATCCTG TCAGGCAGAG TGGGGAGACT TCTCATACCA TCTCGCAACC GACCAAACTT
    ACAGAAGAAT CTTCTGGAAG CAACGCGCCC CAGATTTTCT CAGTAGCAAG GGAGAAAATA
    ACCAGTGACG AGAACAGTAA TGAAAAGTGC TGGGAGAAAA GCATGCCAGA TTCTGTGAAA
    AACCTTAACA TTAACTGCAA CAGCATATTG AAAAACCATC AGCACGGCCC TCCTCAGAGC
    CAGTTTTATG AAACGTGCAA TTCTATCACA GAGGAAGGTC TGTGTTTGGA AACTGGAATC
    CCTTCTTCAC TGGAAGGAAA GGTGTTCCCT GGAATTCAAC TGGAAATAGA CACATCTCCC
    ATGGGCATGA GTCCATTAGG AACTCAGTCG GCCATCATAG AGACAAGCAG GGCACACCCT
    GAAAGCAACA TGGCAGTGTT TCACTTGCGT TACGAAGTTG ACAGAAGAAT GTCAGACAGT
    TTCTGTCCCC TGTCAGATAA CTTGATTTTG GATGATTGTG GAAACTGTGT ACTACCTGCC
    GTTGGTGAGG AACAAGAGAA AAATTACATG GCATATACTT GCAAACTGAT GGAATTGACA
    AATAACTGTG ACAATAAGAA TAGGCAGCTG CAGTGTGATC ATTGTGACCC CTTGAATGAC
    AAATACTTGT GCTTTGAAGG CTCTTGCCCA AAGGCCGATG TGGTATGTTC AAGTGACAGC
    TTTTGCAGGG AGGATTTTAC TGACAGTCCA CCTGCCAAGA CCTTTCTGAG CCATTTTGAG
    GACTTCCCCG ATAATTGTGA AGAGGTAGAA GAAGATTTGT TCAAAAATAA AAAGGAGCGG
    TCTGCTTGGT TAGTGAGGAG ATTTTGCAAA AATGACAAGG AAGTTAAGAA ATCTGTGTTT
    ACTGGGACAA GGGCAATTGT GAGAACTTTG CCTTCTGGTC ACATTGGACT GGAGGCTTTG
    AGTTACATTG ATCAGAAGAA AAATGGTCTC TTATTGCCTC CTGGGAGAGT CATGGAACGG
    CTGTCAACAA TGGTGGTTAG GCAAGATGGT TCCCAAGGTC TGTCAGAAGC CCAGTGGTAT
    CCGATCTACA GTGCAGTGAG AAGGGGAGAA GCCACAGAAC AGACAATTAG ATCTCTTCTC
    CATTTCTTCG CCAAGCTCCC AGCCTCCGAG ACAGCCCATG AGAGGATCAG TGTCGGTCCG
    TACTTAAAGC AATGTGTCCG AGACACAGTG TGTGAGTATC GTGCCACCTT GCAAAGGACT
    TCCATATCTC AGTATATCAC CGGCTCTCTC CTGGAAGCCA CCACATCTTT AGGAGCAAGA
    AGCAGCCTCC TCAATTCTTT TGGAGGATCC ACGGGACGAA TGATGCTGAA AGAACGCCAA
    CCAGGCACCT CTATGGCCAA CTCCAGTGCC CTTCCTTCAA GTTCAGCTGG GATCAGCAAG
    GAACTGATTG ATCTGAAGCC CCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG
    GAGCAGGAGC AAAATATTTA CCGAAGGGTC TTGCCAGTTG ACTATCTTTT CTTCTTGACC
    CGGGGCTTGG GCACAACAGA ACGCCAGAGG TCCCTGCCCT GCCTCAAAGC ATCCATCTCA
    GCATCCATTC TTACCTCCCA GAAGGGAGAG CACAATGCCC TCGAAGATCT TGTGATGAGA
    TTTAATGAGG TGAGCTCCTG GGTGACGTGG CTGATCCTCG CGGCGGGCTC CATGGAAGAG
    AAGCGGGAAG TCTTCTCATA CTTGGTTCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC
    TACAATGCCG TCATGGAGTT CTTGGCCGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG
    CAGTTTATGG ACCAGTCTGA TCTGGAGACC ATGAGGAGCC TGAAGGACGC CATGGCCCAG
    CACGAGTCTT CCTGCGAGTA CAGGAAGGTG GTGACCCGAG CGCTGCACAT CCCCGGCTGC
    AAGGTGGTTC CCTTCTGCGG GGTGTTTCTG AAAGAGCTCT GTGAGGTGCT GGACGGAGCC
    TCTGGCCTCA TGAAGCTTTG CCCGAGGTAC AATTCGCAGG AAGAAACTCT GGAGTTTGTA
    GCCGATTATA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAGAATGG CTTAAAGAAT
    TCAGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGATCA TCAGGAGCTG CAGTCGAAGT
    CTGGAGACAG AGGAGGAGGA CAGCTCCAGT GAAGGGAGCA GCTCAAGGAA AAATTCCCTG
    AAGGATAAAG CCCGATGGCA GTTTATAATC GGAGATTTGT TGGATTCTGA AAATGACATC
    TTTGAGCAGC CCAAAGAGTG GGACTCTCAT GGATCGGAGG AGCCACAGAA AGCCTTTGAC
    CATGGGACAG AGCTCATCCC ATGGTATGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC
    CTGTTGCAGG GGGCCACGGT CATCCACTAT GATCAGGACA CGCACCTCTC TGCCCGCTGC
    TTCCTCCAGC TTCAGCCTGA CAATAGCACC TTGACCTGGA TAAAACCCAC CACTGCTTCC
    CCAGCCAGTG CTAAAGCAAA ACTTGGTGTG CTCAGTAACA CGTCTGAGCC TGGCAAATTC
    CCGTTACTGG GCAATGCTGG ATTAAGTGGC CTGGTGGAGG GGATCTTGGA TCTGTTTTCA
    GCAAAGGCTG TGTACATGGG ACACCCTAAC ATTGATATAC ACACTGTGTG TGTTCAGAAC
    AAACTGAGTA ACATGTCTCT CTCAGAGACT GGCGTGACAC TGCTCTACGG GCTGCAGACC
    ACGGACAATA GATTACTGCA CTTTGTGGCG CCAAAGCACA CAGCTAAAAT GCTCTTCAGT
    GGATTGTTGG AGCTCACGAG AGCTGTGAGG AAGATGAGGA AGTTCCCTGA CCAAAGACAG
    CATTGGCTGC GGAAACAGTA CGTCAGCCTC TACCAGGAGG ATGGACGATA TGAAGGCCCA
    ACTTTGGCTC AAGCTGTAGA GCTGTTTGGT GGCAGACGAT GGAGTACTCG AAACCCCAGC
    CCTGGGACAT CAGCAAAGAG CGCTGAGAAG CCCAACACCT TGAGTATAAG CAACACCAAG
    AAAAAGAAGA AAATCCTTAT GAGGGGTGAG AGTGGAGAGG TGACGGATGA TGAGATGGCC
    ACCCGCAAGG CCAGAACACA CAAAGAGAGC CGGAGCCGGA GTGGCTCTGA TCCTCAAGAT
    GTCAACGAAC AAGAAGAATC AGAGGCAAAT GCCATCATGA ACCCTCCCAA CCCTCTCCCT
    TCCAGAAGAG CCCACTCCTT GACCACGACT GGGCCCCCTA CCTTGGCTGC CGGGACAGCA
    TCTCCCATCA GGCCAGTGTC CTCTCCTGTG CTGCCTGCTT CCAACAGGAG CCCAACCAGT
    GCTTGGAACA GCAGCAGCGG CTGGCCAGGG AAGATCAAAG GAGGGATGCA GGGCTTTCAG
    AGCTTCATGG TTTCAGACAG CAGCATGAGT TTCGTGGAAT TTGTTGAGCT GTTCAAGTCA
    TTCAGTGTGA GGAGCCGCAA GGACCTGAAG GACCTCTTTG ACATCTACGC CGTACCCTGT
    GACCGAGCTG GCTCTGAGGC AGCCCCCCTC TATACCAACC TGACCATTGA CGAAAATATC
    AGCGGTCTTC AGCCTGACCT AGATTTGTTG ACCAGAAATG TCTCAGATTT GGGATTATTC
    ATCAAGAGTA AACAGCAGCT GTCTGACAAC CAGAGGCAGA TATCTGATGC CATTGCTGCT
    GCCAGTATTG TGACAAATGG CACCGGGGTG GAGAGCACAT CTCTGGGTGT GTTTGGAGTT
    GGTATCCTCC AGCTCAATGA CTTCCTAGTG AATTGCCAAG GAGAACACTA TACTTATGAT
    GAAATCCTCA GCATTATCCA GAAGTTCGAG CCTAGCGTTA GTATGTGTCA TCAAGGCCTA
    ATGTCATTTG AAGGATTTGC AAGGTTTCTA ATGGATAAAG ATAATTTTGC CTCGAAAAAT
    GATGAGTCAC AGGAGAACGT TAAAGAAATG CAGCTGCCCC TCTCCTACTA TTACATTGAA
    TCTTCACACA ATACCTACCT CACGGGCCAT CAGCTCAAAG GAGAATCCTC AGTAGAACTC
    TACAGCCAGG TCCTCCTGCA AGGCTGTAGG AGTGTAGAGT TGGACTGCTG GGACGGAGAT
    GATGGAATGC CCATCATTTA TCATGGACAC ACCCTGACAA CCAAGATCCC CTTCAAGGAA
    GTGGTTGAAG CCATCGATCG CAGTGCCTTC ATCAACTCTG ACCTACCCAT CATCATATCG
    ATTGAGAACC ACTGCTCATT GCCTCAGCAA CGAAAAATGG CAGAAATTTT CAAGACTGTG
    TTTGGAGAAA AACTGGTGGC TAAATTCTTA TTTGAGAGTG ATTTCTCAGA TGATCCTATG
    CTCCCCTCAC CTGACCAACT CAGAAGGAAA GTGCTTCTCA AAAATAAGAA ATTAAAAGCC
    CATCAGACAC CGGTGGATAT CTTAAAGCAA AAGGCTCATC AGCTAGCATC CATGCAAGCT
    CAGGCTTATA ATGGTAGCAA TGCCAACTCC CCACCTGCTA ATAATGAGGA AGAGGAAGAT
    GAAGAGGATG AATATGATTA TGATTATGAA TCCCTCTCTG ATGACAACAT TCTAGAAGAC
    AGACCTGAAA ATAAATCATG TAATGACAAA CTTCAGTTTG AGTATAATGA AGAAATCCCC
    AAGAGGATAA AGAAAGCAGA TAACTCTGCT TGCAACAAAG GGAAGGTTTA TGACATGGAA
    CTGGGAGAAG AATTTTATCT TCCTCAGAAT AAAAAGGAAA GCAGACAGAT TGCACCAGAG
    CTTTCTGACC TTGTCATCTA TTGCCAAGCA GTAAAATTCC CAGGCCTGTC AACTCTAAAT
    GCATCTGGCT CTAGCAGAGG AAAAGAAAGG AAAAGCAGGA AGTCCATTTT TGGCAACAAT
    CCGGGCAGAA TGAGCCCCGG GGAGACAGCA CCATTTAACA AAACATCTGG AAAAAGTTCC
    TGTGAAGGAA TGCGACAGGC CTGGGAGGAC TCTGCTTTCT CCGTCAACCC AACCACGTCC
    CTCAGCGCTA TCATTAGAAC TCCCAAATGC TACCATATCT CCTCACTGAA TGAAAATGCC
    GCCAAACGTC TGTGTCGCAG GTATTCTCAG AAACTCATCC AGCACACCGC CTGCCAGCTG
    CTGCGGACGT ACCCGGCTGC CACCCGCATC GACTCCTCCA ACCCCAACCC CCTCCTGTTC
    TGGCTCCATG GGATACAGCT CGTGGCGCTC AACTACCAGA CAGATGATCT CTCTTTACAT
    TTAAATGCCG CCATGTTTGA GGCCAATGGT GGCTGCGGTT ATGTTCTGAA ACCCCCAGTT
    CTGTGGGACA AGAACTGTTC CATGTATCAG AAGTTCTCCC CCTTGGAAAG AGACCTGGAC
    AGCATGGAGC CTGCCATCTA TTCTTTAACT ATTGTCTCTG GGCAGAACGT GTGCCCGAGT
    AACAGCACGG GGAGCCCATG TATCGAAGTC GATGTCCTGG GCATGGCCCT GGACAGCTGC
    CACTTCCGCA CGAAGCCCAT CCACCGCAAT ACTCTGAACC CCATGTGGAA CGAACAGTTT
    CTTTTCCGGG TTCACTTCGA AGATCTCGTA TTTCTTCGTT TTGCTGTCGT GGAGAACAAC
    AGCTCGGTGG TCACTGCTCA GAGAATCATC TCCCTCAAGG CTCTAAAGCG AGGATATCGA
    CATCTTCAGC TGAGAAATCT CCACAATGAA GTCTTGGAAA TCTCTAGTTT ATTCATAAAC
    AGCAGGAGGA TGGAAGAAAA TTCTTCCGGC AATACTGTGC TGGCCTCTCT GATGTTTAAT
    ACGGAAGAAA GAAAATGTTT GCAGACTCAC AGAGTCACAG TGCATGGCGT CCCAGGCCCA
    GAGCCCTTTA CTGTTTTCTC TATAAACGGA GGCACTAAGG CAAAGCAGCT TCTCCAACAA
    ATTCTCGTTA TGGATCAAGA TACCAAACCT ATTGCCACAG ATTATTTTTT AATGGAGGAA
    AAATATTTCA TATCTAAAGA GAAGAATGAA TGTAGGAAAC AGCCGTTCCA GAGAGCCATT
    GGTCCAGAAG AAGAGATCGT GCAGATTTTA AGCAGCTGGT TTCCAGAAGA GGGCTATGTT
    GGCAGGATTG TCTTAAAAAC CCAGCAGGAA AACCTGGAAG AGAAGAGCAT TGCACAAGAT
    GACAAGGAGA TGCTCCTGAG CTCAGAGGAG GACAGCTTCT TTGTCCAGGT GCATGACGTT
    TCCCCAGAGC AACCACGCAC GGTCATCAAG GCGCCGCGTG TCAGCACCGC GCAGGATGTC
    ATTCAGCAGA CCTTATGCAA AGCCAAATAT TCCTATAGCA TCCTGAGCAA CCCCAACCCG
    AGTGACTATG TGCTTTTGGA AGAGGTGGTG AAAGACACCG CCAACAGGAA ATCTTCCACC
    CCAAAGGCCT CCCAGCGCGT CCTTTTGGAT CAGGAGTGTG TGTTTCAAGC TCAAAGCAAG
    TGGAAAGGTG CAGGAAAATT CATCCTTAAG CTAAAGGAAC AGGTGCAGGC ATCTCGAGAA
    GATAAAAAAA AAGGCATCTC TTTTGCAAGC GAACTCAAGA AGCTCACCAA GACTAAACAG
    CCCCGAGGAC CCACATCACC TTCTCAGGTC TTGGCCTCAG AAAACGTCCA AAACAAGGAA
    GAGAAACCTG TGGGTGGCTT GCCTTCCAGT GACACCATAG ACTACCGACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01621
    Clone ID Related Accession (Same CDS sequence) XM_005225543.4 , NM_001205852.1
    Accession Version XM_005225543.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225543.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACTTCTG AAGAAATGGC AGGTTCTGTT CTCAGACCTG TGAGTCAGAG AAAAGTGATC 
    TCTGCTCAGT CAACTGTAGA TGAAAGTAAT GATAAGGTCT CGGGCATCAG TGTTCCGAAG
    TCTCATCCTG TCAGGCAGAG TGGGGAGACT TCTCATACCA TCTCGCAACC GACCAAACTT
    ACAGAAGAAT CTTCTGGAAG CAACGCGCCC CAGATTTTCT CAGTAGCAAG GGAGAAAATA
    ACCAGTGACG AGAACAGTAA TGAAAAGTGC TGGGAGAAAA GCATGCCAGA TTCTGTGAAA
    AACCTTAACA TTAACTGCAA CAGCATATTG AAAAACCATC AGCACGGCCC TCCTCAGAGC
    CAGTTTTATG AAACGTGCAA TTCTATCACA GAGGAAGGTC TGTGTTTGGA AACTGGAATC
    CCTTCTTCAC TGGAAGGAAA GGTGTTCCCT GGAATTCAAC TGGAAATAGA CACATCTCCC
    ATGGGCATGA GTCCATTAGG AACTCAGTCG GCCATCATAG AGACAAGCAG GGCACACCCT
    GAAAGCAACA TGGCAGTGTT TCACTTGCGT TACGAAGTTG ACAGAAGAAT GTCAGACAGT
    TTCTGTCCCC TGTCAGATAA CTTGATTTTG GATGATTGTG GAAACTGTGT ACTACCTGCC
    GTTGGTGAGG AACAAGAGAA AAATTACATG GCATATACTT GCAAACTGAT GGAATTGACA
    AATAACTGTG ACAATAAGAA TAGGCAGCTG CAGTGTGATC ATTGTGACCC CTTGAATGAC
    AAATACTTGT GCTTTGAAGG CTCTTGCCCA AAGGCCGATG TGGTATGTTC AAGTGACAGC
    TTTTGCAGGG AGGATTTTAC TGACAGTCCA CCTGCCAAGA CCTTTCTGAG CCATTTTGAG
    GACTTCCCCG ATAATTGTGA AGAGGTAGAA GAAGATTTGT TCAAAAATAA AAAGGAGCGG
    TCTGCTTGGT TAGTGAGGAG ATTTTGCAAA AATGACAAGG AAGTTAAGAA ATCTGTGTTT
    ACTGGGACAA GGGCAATTGT GAGAACTTTG CCTTCTGGTC ACATTGGACT GGAGGCTTTG
    AGTTACATTG ATCAGAAGAA AAATGGTCTC TTATTGCCTC CTGGGAGAGT CATGGAACGG
    CTGTCAACAA TGGTGGTTAG GCAAGATGGT TCCCAAGGTC TGTCAGAAGC CCAGTGGTAT
    CCGATCTACA GTGCAGTGAG AAGGGGAGAA GCCACAGAAC AGACAATTAG ATCTCTTCTC
    CATTTCTTCG CCAAGCTCCC AGCCTCCGAG ACAGCCCATG AGAGGATCAG TGTCGGTCCG
    TACTTAAAGC AATGTGTCCG AGACACAGTG TGTGAGTATC GTGCCACCTT GCAAAGGACT
    TCCATATCTC AGTATATCAC CGGCTCTCTC CTGGAAGCCA CCACATCTTT AGGAGCAAGA
    AGCAGCCTCC TCAATTCTTT TGGAGGATCC ACGGGACGAA TGATGCTGAA AGAACGCCAA
    CCAGGCACCT CTATGGCCAA CTCCAGTGCC CTTCCTTCAA GTTCAGCTGG GATCAGCAAG
    GAACTGATTG ATCTGAAGCC CCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG
    GAGCAGGAGC AAAATATTTA CCGAAGGGTC TTGCCAGTTG ACTATCTTTT CTTCTTGACC
    CGGGGCTTGG GCACAACAGA ACGCCAGAGG TCCCTGCCCT GCCTCAAAGC ATCCATCTCA
    GCATCCATTC TTACCTCCCA GAAGGGAGAG CACAATGCCC TCGAAGATCT TGTGATGAGA
    TTTAATGAGG TGAGCTCCTG GGTGACGTGG CTGATCCTCG CGGCGGGCTC CATGGAAGAG
    AAGCGGGAAG TCTTCTCATA CTTGGTTCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC
    TACAATGCCG TCATGGAGTT CTTGGCCGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG
    CAGTTTATGG ACCAGTCTGA TCTGGAGACC ATGAGGAGCC TGAAGGACGC CATGGCCCAG
    CACGAGTCTT CCTGCGAGTA CAGGAAGGTG GTGACCCGAG CGCTGCACAT CCCCGGCTGC
    AAGGTGGTTC CCTTCTGCGG GGTGTTTCTG AAAGAGCTCT GTGAGGTGCT GGACGGAGCC
    TCTGGCCTCA TGAAGCTTTG CCCGAGGTAC AATTCGCAGG AAGAAACTCT GGAGTTTGTA
    GCCGATTATA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAGAATGG CTTAAAGAAT
    TCAGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGATCA TCAGGAGCTG CAGTCGAAGT
    CTGGAGACAG AGGAGGAGGA CAGCTCCAGT GAAGGGAGCA GCTCAAGGAA AAATTCCCTG
    AAGGATAAAG CCCGATGGCA GTTTATAATC GGAGATTTGT TGGATTCTGA AAATGACATC
    TTTGAGCAGC CCAAAGAGTG GGACTCTCAT GGATCGGAGG AGCCACAGAA AGCCTTTGAC
    CATGGGACAG AGCTCATCCC ATGGTATGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC
    CTGTTGCAGG GGGCCACGGT CATCCACTAT GATCAGGACA CGCACCTCTC TGCCCGCTGC
    TTCCTCCAGC TTCAGCCTGA CAATAGCACC TTGACCTGGA TAAAACCCAC CACTGCTTCC
    CCAGCCAGTG CTAAAGCAAA ACTTGGTGTG CTCAGTAACA CGTCTGAGCC TGGCAAATTC
    CCGTTACTGG GCAATGCTGG ATTAAGTGGC CTGGTGGAGG GGATCTTGGA TCTGTTTTCA
    GCAAAGGCTG TGTACATGGG ACACCCTAAC ATTGATATAC ACACTGTGTG TGTTCAGAAC
    AAACTGAGTA ACATGTCTCT CTCAGAGACT GGCGTGACAC TGCTCTACGG GCTGCAGACC
    ACGGACAATA GATTACTGCA CTTTGTGGCG CCAAAGCACA CAGCTAAAAT GCTCTTCAGT
    GGATTGTTGG AGCTCACGAG AGCTGTGAGG AAGATGAGGA AGTTCCCTGA CCAAAGACAG
    CATTGGCTGC GGAAACAGTA CGTCAGCCTC TACCAGGAGG ATGGACGATA TGAAGGCCCA
    ACTTTGGCTC AAGCTGTAGA GCTGTTTGGT GGCAGACGAT GGAGTACTCG AAACCCCAGC
    CCTGGGACAT CAGCAAAGAG CGCTGAGAAG CCCAACACCT TGAGTATAAG CAACACCAAG
    AAAAAGAAGA AAATCCTTAT GAGGGGTGAG AGTGGAGAGG TGACGGATGA TGAGATGGCC
    ACCCGCAAGG CCAGAACACA CAAAGAGAGC CGGAGCCGGA GTGGCTCTGA TCCTCAAGAT
    GTCAACGAAC AAGAAGAATC AGAGGCAAAT GCCATCATGA ACCCTCCCAA CCCTCTCCCT
    TCCAGAAGAG CCCACTCCTT GACCACGACT GGGCCCCCTA CCTTGGCTGC CGGGACAGCA
    TCTCCCATCA GGCCAGTGTC CTCTCCTGTG CTGCCTGCTT CCAACAGGAG CCCAACCAGT
    GCTTGGAACA GCAGCAGCGG CTGGCCAGGG AAGATCAAAG GAGGGATGCA GGGCTTTCAG
    AGCTTCATGG TTTCAGACAG CAGCATGAGT TTCGTGGAAT TTGTTGAGCT GTTCAAGTCA
    TTCAGTGTGA GGAGCCGCAA GGACCTGAAG GACCTCTTTG ACATCTACGC CGTACCCTGT
    GACCGAGCTG GCTCTGAGGC AGCCCCCCTC TATACCAACC TGACCATTGA CGAAAATATC
    AGCGGTCTTC AGCCTGACCT AGATTTGTTG ACCAGAAATG TCTCAGATTT GGGATTATTC
    ATCAAGACTA AACAGCAGCT GTCTGACAAC CAGAGGCAGA TATCTGATGC CATTGCTGCT
    GCCAGTATTG TGACAAATGG CACCGGGGTG GAGAGCACAT CTCTGGGTGT GTTTGGAGTT
    GGTATCCTCC AGCTCAATGA CTTCCTAGTG AATTGCCAAG GAGAACACTA TACTTATGAT
    GAAATCCTCA GCATTATCCA GAAGTTCGAG CCTAGCGTTA GTATGTGTCA TCAAGGCCTA
    ATGTCATTTG AAGGATTTGC AAGGTTTCTA ATGGATAAAG ATAATTTTGC CTCGAAAAAT
    GATGAGTCAC AGGAGAACGT TAAAGAAATG CAGCTGCCCC TCTCCTACTA TTACATTGAA
    TCTTCACACA ATACCTACCT CACGGGCCAT CAGCTCAAAG GAGAATCCTC AGTAGAACTC
    TACAGCCAGG TCCTCCTGCA AGGCTGTAGG AGTGTAGAGT TGGACTGCTG GGACGGAGAT
    GATGGAATGC CCATCATTTA TCATGGACAC ACCCTGACAA CCAAGATCCC CTTCAAGGAA
    GTGGTTGAAG CCATCGATCG CAGTGCCTTC ATCAACTCTG ACCTACCCAT CATCATATCG
    ATTGAGAACC ACTGCTCATT GCCTCAGCAA CGAAAAATGG CAGAAATTTT CAAGACTGTG
    TTTGGAGAAA AACTGGTGGC TAAATTCTTA TTTGAGAGTG ATTTCTCAGA TGATCCTATG
    CTCCCCTCAC CTGACCAACT CAGAAGGAAA GTGCTTCTCA AAAATAAGAA ATTAAAAGCC
    CATCAGACAC CGGTGGATAT CTTAAAGCAA AAGGCTCATC AGCTAGCATC CATGCAAGCT
    CAGGCTTATA ATGGTAGCAA TGCCAACTCC CCACCTGCTA ATAATGAGGA AGAGGAAGAT
    GAAGAGGATG AATATGATTA TGATTATGAA TCCCTCTCTG ATGACAACAT TCTAGAAGAC
    AGACCTGAAA ATAAATCATG TAATGACAAA CTTCAGTTTG AGTATAATGA AGAAATCCCC
    AAGAGGATAA AGAAAGCAGA TAACTCTGCT TGCAACAAAG GGAAGGTTTA TGACATGGAA
    CTGGGAGAAG AATTTTATCT TCCTCAGAAT AAAAAGGAAA GCAGACAGAT TGCACCAGAG
    CTTTCTGACC TTGTCATCTA TTGCCAAGCA GTAAAATTCC CAGGCCTGTC AACTCTAAAT
    GCATCTGGCT CTAGCAGAGG AAAAGAAAGG AAAAGCAGGA AGTCCATTTT TGGCAACAAT
    CCGGGCAGAA TGAGCCCCGG GGAGACAGCA CCATTTAACA AAACATCTGG AAAAAGTTCC
    TGTGAAGGAA TGCGACAGGC CTGGGAGGAC TCTGCTTTCT CCGTCAACCC AACCACGTCC
    CTCAGCGCTA TCATTAGAAC TCCCAAATGC TACCATATCT CCTCACTGAA TGAAAATGCC
    GCCAAACGTC TGTGTCGCAG GTATTCTCAG AAACTCATCC AGCACACCGC CTGCCAGCTG
    CTGCGGACGT ACCCGGCTGC CACCCGCATC GACTCCTCCA ACCCCAACCC CCTCCTGTTC
    TGGCTCCATG GGATACAGCT CGTGGCGCTC AACTACCAGA CAGATGATCT CTCTTTACAT
    TTAAATGCCG CCATGTTTGA GGCCAATGGT GGCTGCGGTT ATGTTCTGAA ACCCCCAGTT
    CTGTGGGACA AGAACTGTTC CATGTATCAG AAGTTCTCCC CCTTGGAAAG AGACCTGGAC
    AGCATGGAGC CTGCCATCTA TTCTTTAACT ATTGTCTCTG GGCAGAACGT GTGCCCGAGT
    AACAGCACGG GGAGCCCATG TATCGAAGTC GATGTCCTGG GCATGGCCCT GGACAGCTGC
    CACTTCCGCA CGAAGCCCAT CCACCGCAAT ACTCTGAACC CCATGTGGAA CGAACAGTTT
    CTTTTCCGGG TTCACTTCGA AGATCTCGTA TTTCTTCGTT TTGCTGTCGT GGAGAACAAC
    AGCTCGGTGG TCACTGCTCA GAGAATCATC TCCCTCAAGG CTCTAAAGCG AGGATATCGA
    CATCTTCAGC TGAGAAATCT CCACAATGAA GTCTTGGAAA TCTCTAGTTT ATTCATAAAC
    AGCAGGAGGA TGGAAGAAAA TTCTTCCGGC AATACTGTGC TGGCCTCTCT GATGTTTAAT
    ACGGAAGAAA GAAAATGTTT GCAGACTCAC AGAGTCACAG TGCATGGCGT CCCAGGCCCA
    GAGCCCTTTA CTGTTTTCTC TATAAACGGA GGCACTAAGG CAAAGCAGCT TCTCCAACAA
    ATTCTCGTTA TGGATCAAGA TACCAAACCT ATTGCCACAG ATTATTTTTT AATGGAGGAA
    AAATATTTCA TATCTAAAGA GAAGAATGAA TGTAGGAAAC AGCCGTTCCA GAGAGCCATT
    GGTCCAGAAG AAGAGATCGT GCAGATTTTA AGCAGCTGGT TTCCAGAAGA GGGCTATGTT
    GGCAGGATTG TCTTAAAAAC CCAGCAGGAA AACCTGGAAG AGAAGAGCAT TGCACAAGAT
    GACAAGGAGA TGCTCCTGAG CTCAGAGGAG GACAGCTTCT TTGTCCAGGT GCATGACGTT
    TCCCCAGAGC AACCACGCAC GGTCATCAAG GCGCCGCGTG TCAGCACCGC GCAGGATGTC
    ATTCAGCAGA CCTTATGCAA AGCCAAATAT TCCTATAGCA TCCTGAGCAA CCCCAACCCG
    AGTGACTATG TGCTTTTGGA AGAGGTGGTG AAAGACACCG CCAACAGGAA ATCTTCCACC
    CCAAAGGCCT CCCAGCGCGT CCTTTTGGAT CAGGAGTGTG TGTTTCAAGC TCAAAGCAAG
    TGGAAAGGTG CAGGAAAATT CATCCTTAAG CTAAAGGAAC AGGTGCAGGC ATCTCGAGAA
    GATAAAAAAA AAGGCATCTC TTTTGCAAGC GAACTCAAGA AGCTCACCAA GACTAAACAG
    CCCCGAGGAC CCACATCACC TTCTCAGGTC TTGGCCTCAG AAAACGTCCA AAACAAGGAA
    GAGAAACCTG TGGGTGGCTT GCCTTCCAGT GACACCATAG ACTACCGACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225600.2
    CDS539..7432
    Translation

    Target ORF information:

    RefSeq Version XM_005225543.4
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C epsilon 1 (PLCE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225543.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACTTCTG AAGAAATGGC AGGTTCTGTT CTCAGACCTG TGAGTCAGAG AAAAGTGATC 
    TCTGCTCAGT CAACTGTAGA TGAAAGTAAT GATAAGGTCT CGGGCATCAG TGTTCCGAAG
    TCTCATCCTG TCAGGCAGAG TGGGGAGACT TCTCATACCA TCTCGCAACC GACCAAACTT
    ACAGAAGAAT CTTCTGGAAG CAACGCGCCC CAGATTTTCT CAGTAGCAAG GGAGAAAATA
    ACCAGTGACG AGAACAGTAA TGAAAAGTGC TGGGAGAAAA GCATGCCAGA TTCTGTGAAA
    AACCTTAACA TTAACTGCAA CAGCATATTG AAAAACCATC AGCACGGCCC TCCTCAGAGC
    CAGTTTTATG AAACGTGCAA TTCTATCACA GAGGAAGGTC TGTGTTTGGA AACTGGAATC
    CCTTCTTCAC TGGAAGGAAA GGTGTTCCCT GGAATTCAAC TGGAAATAGA CACATCTCCC
    ATGGGCATGA GTCCATTAGG AACTCAGTCG GCCATCATAG AGACAAGCAG GGCACACCCT
    GAAAGCAACA TGGCAGTGTT TCACTTGCGT TACGAAGTTG ACAGAAGAAT GTCAGACAGT
    TTCTGTCCCC TGTCAGATAA CTTGATTTTG GATGATTGTG GAAACTGTGT ACTACCTGCC
    GTTGGTGAGG AACAAGAGAA AAATTACATG GCATATACTT GCAAACTGAT GGAATTGACA
    AATAACTGTG ACAATAAGAA TAGGCAGCTG CAGTGTGATC ATTGTGACCC CTTGAATGAC
    AAATACTTGT GCTTTGAAGG CTCTTGCCCA AAGGCCGATG TGGTATGTTC AAGTGACAGC
    TTTTGCAGGG AGGATTTTAC TGACAGTCCA CCTGCCAAGA CCTTTCTGAG CCATTTTGAG
    GACTTCCCCG ATAATTGTGA AGAGGTAGAA GAAGATTTGT TCAAAAATAA AAAGGAGCGG
    TCTGCTTGGT TAGTGAGGAG ATTTTGCAAA AATGACAAGG AAGTTAAGAA ATCTGTGTTT
    ACTGGGACAA GGGCAATTGT GAGAACTTTG CCTTCTGGTC ACATTGGACT GGAGGCTTTG
    AGTTACATTG ATCAGAAGAA AAATGGTCTC TTATTGCCTC CTGGGAGAGT CATGGAACGG
    CTGTCAACAA TGGTGGTTAG GCAAGATGGT TCCCAAGGTC TGTCAGAAGC CCAGTGGTAT
    CCGATCTACA GTGCAGTGAG AAGGGGAGAA GCCACAGAAC AGACAATTAG ATCTCTTCTC
    CATTTCTTCG CCAAGCTCCC AGCCTCCGAG ACAGCCCATG AGAGGATCAG TGTCGGTCCG
    TACTTAAAGC AATGTGTCCG AGACACAGTG TGTGAGTATC GTGCCACCTT GCAAAGGACT
    TCCATATCTC AGTATATCAC CGGCTCTCTC CTGGAAGCCA CCACATCTTT AGGAGCAAGA
    AGCAGCCTCC TCAATTCTTT TGGAGGATCC ACGGGACGAA TGATGCTGAA AGAACGCCAA
    CCAGGCACCT CTATGGCCAA CTCCAGTGCC CTTCCTTCAA GTTCAGCTGG GATCAGCAAG
    GAACTGATTG ATCTGAAGCC CCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG
    GAGCAGGAGC AAAATATTTA CCGAAGGGTC TTGCCAGTTG ACTATCTTTT CTTCTTGACC
    CGGGGCTTGG GCACAACAGA ACGCCAGAGG TCCCTGCCCT GCCTCAAAGC ATCCATCTCA
    GCATCCATTC TTACCTCCCA GAAGGGAGAG CACAATGCCC TCGAAGATCT TGTGATGAGA
    TTTAATGAGG TGAGCTCCTG GGTGACGTGG CTGATCCTCG CGGCGGGCTC CATGGAAGAG
    AAGCGGGAAG TCTTCTCATA CTTGGTTCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC
    TACAATGCCG TCATGGAGTT CTTGGCCGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG
    CAGTTTATGG ACCAGTCTGA TCTGGAGACC ATGAGGAGCC TGAAGGACGC CATGGCCCAG
    CACGAGTCTT CCTGCGAGTA CAGGAAGGTG GTGACCCGAG CGCTGCACAT CCCCGGCTGC
    AAGGTGGTTC CCTTCTGCGG GGTGTTTCTG AAAGAGCTCT GTGAGGTGCT GGACGGAGCC
    TCTGGCCTCA TGAAGCTTTG CCCGAGGTAC AATTCGCAGG AAGAAACTCT GGAGTTTGTA
    GCCGATTATA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAGAATGG CTTAAAGAAT
    TCAGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGATCA TCAGGAGCTG CAGTCGAAGT
    CTGGAGACAG AGGAGGAGGA CAGCTCCAGT GAAGGGAGCA GCTCAAGGAA AAATTCCCTG
    AAGGATAAAG CCCGATGGCA GTTTATAATC GGAGATTTGT TGGATTCTGA AAATGACATC
    TTTGAGCAGC CCAAAGAGTG GGACTCTCAT GGATCGGAGG AGCCACAGAA AGCCTTTGAC
    CATGGGACAG AGCTCATCCC ATGGTATGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC
    CTGTTGCAGG GGGCCACGGT CATCCACTAT GATCAGGACA CGCACCTCTC TGCCCGCTGC
    TTCCTCCAGC TTCAGCCTGA CAATAGCACC TTGACCTGGA TAAAACCCAC CACTGCTTCC
    CCAGCCAGTG CTAAAGCAAA ACTTGGTGTG CTCAGTAACA CGTCTGAGCC TGGCAAATTC
    CCGTTACTGG GCAATGCTGG ATTAAGTGGC CTGGTGGAGG GGATCTTGGA TCTGTTTTCA
    GCAAAGGCTG TGTACATGGG ACACCCTAAC ATTGATATAC ACACTGTGTG TGTTCAGAAC
    AAACTGAGTA ACATGTCTCT CTCAGAGACT GGCGTGACAC TGCTCTACGG GCTGCAGACC
    ACGGACAATA GATTACTGCA CTTTGTGGCG CCAAAGCACA CAGCTAAAAT GCTCTTCAGT
    GGATTGTTGG AGCTCACGAG AGCTGTGAGG AAGATGAGGA AGTTCCCTGA CCAAAGACAG
    CATTGGCTGC GGAAACAGTA CGTCAGCCTC TACCAGGAGG ATGGACGATA TGAAGGCCCA
    ACTTTGGCTC AAGCTGTAGA GCTGTTTGGT GGCAGACGAT GGAGTACTCG AAACCCCAGC
    CCTGGGACAT CAGCAAAGAG CGCTGAGAAG CCCAACACCT TGAGTATAAG CAACACCAAG
    AAAAAGAAGA AAATCCTTAT GAGGGGTGAG AGTGGAGAGG TGACGGATGA TGAGATGGCC
    ACCCGCAAGG CCAGAACACA CAAAGAGAGC CGGAGCCGGA GTGGCTCTGA TCCTCAAGAT
    GTCAACGAAC AAGAAGAATC AGAGGCAAAT GCCATCATGA ACCCTCCCAA CCCTCTCCCT
    TCCAGAAGAG CCCACTCCTT GACCACGACT GGGCCCCCTA CCTTGGCTGC CGGGACAGCA
    TCTCCCATCA GGCCAGTGTC CTCTCCTGTG CTGCCTGCTT CCAACAGGAG CCCAACCAGT
    GCTTGGAACA GCAGCAGCGG CTGGCCAGGG AAGATCAAAG GAGGGATGCA GGGCTTTCAG
    AGCTTCATGG TTTCAGACAG CAGCATGAGT TTCGTGGAAT TTGTTGAGCT GTTCAAGTCA
    TTCAGTGTGA GGAGCCGCAA GGACCTGAAG GACCTCTTTG ACATCTACGC CGTACCCTGT
    GACCGAGCTG GCTCTGAGGC AGCCCCCCTC TATACCAACC TGACCATTGA CGAAAATATC
    AGCGGTCTTC AGCCTGACCT AGATTTGTTG ACCAGAAATG TCTCAGATTT GGGATTATTC
    ATCAAGACTA AACAGCAGCT GTCTGACAAC CAGAGGCAGA TATCTGATGC CATTGCTGCT
    GCCAGTATTG TGACAAATGG CACCGGGGTG GAGAGCACAT CTCTGGGTGT GTTTGGAGTT
    GGTATCCTCC AGCTCAATGA CTTCCTAGTG AATTGCCAAG GAGAACACTA TACTTATGAT
    GAAATCCTCA GCATTATCCA GAAGTTCGAG CCTAGCGTTA GTATGTGTCA TCAAGGCCTA
    ATGTCATTTG AAGGATTTGC AAGGTTTCTA ATGGATAAAG ATAATTTTGC CTCGAAAAAT
    GATGAGTCAC AGGAGAACGT TAAAGAAATG CAGCTGCCCC TCTCCTACTA TTACATTGAA
    TCTTCACACA ATACCTACCT CACGGGCCAT CAGCTCAAAG GAGAATCCTC AGTAGAACTC
    TACAGCCAGG TCCTCCTGCA AGGCTGTAGG AGTGTAGAGT TGGACTGCTG GGACGGAGAT
    GATGGAATGC CCATCATTTA TCATGGACAC ACCCTGACAA CCAAGATCCC CTTCAAGGAA
    GTGGTTGAAG CCATCGATCG CAGTGCCTTC ATCAACTCTG ACCTACCCAT CATCATATCG
    ATTGAGAACC ACTGCTCATT GCCTCAGCAA CGAAAAATGG CAGAAATTTT CAAGACTGTG
    TTTGGAGAAA AACTGGTGGC TAAATTCTTA TTTGAGAGTG ATTTCTCAGA TGATCCTATG
    CTCCCCTCAC CTGACCAACT CAGAAGGAAA GTGCTTCTCA AAAATAAGAA ATTAAAAGCC
    CATCAGACAC CGGTGGATAT CTTAAAGCAA AAGGCTCATC AGCTAGCATC CATGCAAGCT
    CAGGCTTATA ATGGTAGCAA TGCCAACTCC CCACCTGCTA ATAATGAGGA AGAGGAAGAT
    GAAGAGGATG AATATGATTA TGATTATGAA TCCCTCTCTG ATGACAACAT TCTAGAAGAC
    AGACCTGAAA ATAAATCATG TAATGACAAA CTTCAGTTTG AGTATAATGA AGAAATCCCC
    AAGAGGATAA AGAAAGCAGA TAACTCTGCT TGCAACAAAG GGAAGGTTTA TGACATGGAA
    CTGGGAGAAG AATTTTATCT TCCTCAGAAT AAAAAGGAAA GCAGACAGAT TGCACCAGAG
    CTTTCTGACC TTGTCATCTA TTGCCAAGCA GTAAAATTCC CAGGCCTGTC AACTCTAAAT
    GCATCTGGCT CTAGCAGAGG AAAAGAAAGG AAAAGCAGGA AGTCCATTTT TGGCAACAAT
    CCGGGCAGAA TGAGCCCCGG GGAGACAGCA CCATTTAACA AAACATCTGG AAAAAGTTCC
    TGTGAAGGAA TGCGACAGGC CTGGGAGGAC TCTGCTTTCT CCGTCAACCC AACCACGTCC
    CTCAGCGCTA TCATTAGAAC TCCCAAATGC TACCATATCT CCTCACTGAA TGAAAATGCC
    GCCAAACGTC TGTGTCGCAG GTATTCTCAG AAACTCATCC AGCACACCGC CTGCCAGCTG
    CTGCGGACGT ACCCGGCTGC CACCCGCATC GACTCCTCCA ACCCCAACCC CCTCCTGTTC
    TGGCTCCATG GGATACAGCT CGTGGCGCTC AACTACCAGA CAGATGATCT CTCTTTACAT
    TTAAATGCCG CCATGTTTGA GGCCAATGGT GGCTGCGGTT ATGTTCTGAA ACCCCCAGTT
    CTGTGGGACA AGAACTGTTC CATGTATCAG AAGTTCTCCC CCTTGGAAAG AGACCTGGAC
    AGCATGGAGC CTGCCATCTA TTCTTTAACT ATTGTCTCTG GGCAGAACGT GTGCCCGAGT
    AACAGCACGG GGAGCCCATG TATCGAAGTC GATGTCCTGG GCATGGCCCT GGACAGCTGC
    CACTTCCGCA CGAAGCCCAT CCACCGCAAT ACTCTGAACC CCATGTGGAA CGAACAGTTT
    CTTTTCCGGG TTCACTTCGA AGATCTCGTA TTTCTTCGTT TTGCTGTCGT GGAGAACAAC
    AGCTCGGTGG TCACTGCTCA GAGAATCATC TCCCTCAAGG CTCTAAAGCG AGGATATCGA
    CATCTTCAGC TGAGAAATCT CCACAATGAA GTCTTGGAAA TCTCTAGTTT ATTCATAAAC
    AGCAGGAGGA TGGAAGAAAA TTCTTCCGGC AATACTGTGC TGGCCTCTCT GATGTTTAAT
    ACGGAAGAAA GAAAATGTTT GCAGACTCAC AGAGTCACAG TGCATGGCGT CCCAGGCCCA
    GAGCCCTTTA CTGTTTTCTC TATAAACGGA GGCACTAAGG CAAAGCAGCT TCTCCAACAA
    ATTCTCGTTA TGGATCAAGA TACCAAACCT ATTGCCACAG ATTATTTTTT AATGGAGGAA
    AAATATTTCA TATCTAAAGA GAAGAATGAA TGTAGGAAAC AGCCGTTCCA GAGAGCCATT
    GGTCCAGAAG AAGAGATCGT GCAGATTTTA AGCAGCTGGT TTCCAGAAGA GGGCTATGTT
    GGCAGGATTG TCTTAAAAAC CCAGCAGGAA AACCTGGAAG AGAAGAGCAT TGCACAAGAT
    GACAAGGAGA TGCTCCTGAG CTCAGAGGAG GACAGCTTCT TTGTCCAGGT GCATGACGTT
    TCCCCAGAGC AACCACGCAC GGTCATCAAG GCGCCGCGTG TCAGCACCGC GCAGGATGTC
    ATTCAGCAGA CCTTATGCAA AGCCAAATAT TCCTATAGCA TCCTGAGCAA CCCCAACCCG
    AGTGACTATG TGCTTTTGGA AGAGGTGGTG AAAGACACCG CCAACAGGAA ATCTTCCACC
    CCAAAGGCCT CCCAGCGCGT CCTTTTGGAT CAGGAGTGTG TGTTTCAAGC TCAAAGCAAG
    TGGAAAGGTG CAGGAAAATT CATCCTTAAG CTAAAGGAAC AGGTGCAGGC ATCTCGAGAA
    GATAAAAAAA AAGGCATCTC TTTTGCAAGC GAACTCAAGA AGCTCACCAA GACTAAACAG
    CCCCGAGGAC CCACATCACC TTCTCAGGTC TTGGCCTCAG AAAACGTCCA AAACAAGGAA
    GAGAAACCTG TGGGTGGCTT GCCTTCCAGT GACACCATAG ACTACCGACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33573
    Clone ID Related Accession (Same CDS sequence) XM_005225543.3 , NM_001205852.2
    Accession Version XM_005225543.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225543.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACTTCTG AAGAAATGGC AGGTTCTGTT CTCAGACCTG TGAGTCAGAG AAAAGTGATC 
    TCTGCTCAGT CAACTGTAGA TGAAAGTAAT GATAAGGTCT CGGGCATCAG TGTTCCGAAG
    TCTCATCCTG TCAGGCAGAG TGGGGAGACT TCTCATACCA TCTCGCAACC GACCAAACTT
    ACAGAAGAAT CTTCTGGAAG CAACGCGCCC CAGATTTTCT CAGTAGCAAG GGAGAAAATA
    ACCAGTGACG AGAACAGTAA TGAAAAGTGC TGGGAGAAAA GCATGCCAGA TTCTGTGAAA
    AACCTTAACA TTAACTGCAA CAGCATATTG AAAAACCATC AGCACGGCCC TCCTCAGAGC
    CAGTTTTATG AAACGTGCAA TTCTATCACA GAGGAAGGTC TGTGTTTGGA AACTGGAATC
    CCTTCTTCAC TGGAAGGAAA GGTGTTCCCT GGAATTCAAC TGGAAATAGA CACATCTCCC
    ATGGGCATGA GTCCATTAGG AACTCAGTCG GCCATCATAG AGACAAGCAG GGCACACCCT
    GAAAGCAACA TGGCAGTGTT TCACTTGCGT TACGAAGTTG ACAGAAGAAT GTCAGACAGT
    TTCTGTCCCC TGTCAGATAA CTTGATTTTG GATGATTGTG GAAACTGTGT ACTACCTGCC
    GTTGGTGAGG AACAAGAGAA AAATTACATG GCATATACTT GCAAACTGAT GGAATTGACA
    AATAACTGTG ACAATAAGAA TAGGCAGCTG CAGTGTGATC ATTGTGACCC CTTGAATGAC
    AAATACTTGT GCTTTGAAGG CTCTTGCCCA AAGGCCGATG TGGTATGTTC AAGTGACAGC
    TTTTGCAGGG AGGATTTTAC TGACAGTCCA CCTGCCAAGA CCTTTCTGAG CCATTTTGAG
    GACTTCCCCG ATAATTGTGA AGAGGTAGAA GAAGATTTGT TCAAAAATAA AAAGGAGCGG
    TCTGCTTGGT TAGTGAGGAG ATTTTGCAAA AATGACAAGG AAGTTAAGAA ATCTGTGTTT
    ACTGGGACAA GGGCAATTGT GAGAACTTTG CCTTCTGGTC ACATTGGACT GGAGGCTTTG
    AGTTACATTG ATCAGAAGAA AAATGGTCTC TTATTGCCTC CTGGGAGAGT CATGGAACGG
    CTGTCAACAA TGGTGGTTAG GCAAGATGGT TCCCAAGGTC TGTCAGAAGC CCAGTGGTAT
    CCGATCTACA GTGCAGTGAG AAGGGGAGAA GCCACAGAAC AGACAATTAG ATCTCTTCTC
    CATTTCTTCG CCAAGCTCCC AGCCTCCGAG ACAGCCCATG AGAGGATCAG TGTCGGTCCG
    TACTTAAAGC AATGTGTCCG AGACACAGTG TGTGAGTATC GTGCCACCTT GCAAAGGACT
    TCCATATCTC AGTATATCAC CGGCTCTCTC CTGGAAGCCA CCACATCTTT AGGAGCAAGA
    AGCAGCCTCC TCAATTCTTT TGGAGGATCC ACGGGACGAA TGATGCTGAA AGAACGCCAA
    CCAGGCACCT CTATGGCCAA CTCCAGTGCC CTTCCTTCAA GTTCAGCTGG GATCAGCAAG
    GAACTGATTG ATCTGAAGCC CCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG
    GAGCAGGAGC AAAATATTTA CCGAAGGGTC TTGCCAGTTG ACTATCTTTT CTTCTTGACC
    CGGGGCTTGG GCACAACAGA ACGCCAGAGG TCCCTGCCCT GCCTCAAAGC ATCCATCTCA
    GCATCCATTC TTACCTCCCA GAAGGGAGAG CACAATGCCC TCGAAGATCT TGTGATGAGA
    TTTAATGAGG TGAGCTCCTG GGTGACGTGG CTGATCCTCG CGGCGGGCTC CATGGAAGAG
    AAGCGGGAAG TCTTCTCATA CTTGGTTCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC
    TACAATGCCG TCATGGAGTT CTTGGCCGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG
    CAGTTTATGG ACCAGTCTGA TCTGGAGACC ATGAGGAGCC TGAAGGACGC CATGGCCCAG
    CACGAGTCTT CCTGCGAGTA CAGGAAGGTG GTGACCCGAG CGCTGCACAT CCCCGGCTGC
    AAGGTGGTTC CCTTCTGCGG GGTGTTTCTG AAAGAGCTCT GTGAGGTGCT GGACGGAGCC
    TCTGGCCTCA TGAAGCTTTG CCCGAGGTAC AATTCGCAGG AAGAAACTCT GGAGTTTGTA
    GCCGATTATA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAGAATGG CTTAAAGAAT
    TCAGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGATCA TCAGGAGCTG CAGTCGAAGT
    CTGGAGACAG AGGAGGAGGA CAGCTCCAGT GAAGGGAGCA GCTCAAGGAA AAATTCCCTG
    AAGGATAAAG CCCGATGGCA GTTTATAATC GGAGATTTGT TGGATTCTGA AAATGACATC
    TTTGAGCAGC CCAAAGAGTG GGACTCTCAT GGATCGGAGG AGCCACAGAA AGCCTTTGAC
    CATGGGACAG AGCTCATCCC ATGGTATGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC
    CTGTTGCAGG GGGCCACGGT CATCCACTAT GATCAGGACA CGCACCTCTC TGCCCGCTGC
    TTCCTCCAGC TTCAGCCTGA CAATAGCACC TTGACCTGGA TAAAACCCAC CACTGCTTCC
    CCAGCCAGTG CTAAAGCAAA ACTTGGTGTG CTCAGTAACA CGTCTGAGCC TGGCAAATTC
    CCGTTACTGG GCAATGCTGG ATTAAGTGGC CTGGTGGAGG GGATCTTGGA TCTGTTTTCA
    GCAAAGGCTG TGTACATGGG ACACCCTAAC ATTGATATAC ACACTGTGTG TGTTCAGAAC
    AAACTGAGTA ACATGTCTCT CTCAGAGACT GGCGTGACAC TGCTCTACGG GCTGCAGACC
    ACGGACAATA GATTACTGCA CTTTGTGGCG CCAAAGCACA CAGCTAAAAT GCTCTTCAGT
    GGATTGTTGG AGCTCACGAG AGCTGTGAGG AAGATGAGGA AGTTCCCTGA CCAAAGACAG
    CATTGGCTGC GGAAACAGTA CGTCAGCCTC TACCAGGAGG ATGGACGATA TGAAGGCCCA
    ACTTTGGCTC AAGCTGTAGA GCTGTTTGGT GGCAGACGAT GGAGTACTCG AAACCCCAGC
    CCTGGGACAT CAGCAAAGAG CGCTGAGAAG CCCAACACCT TGAGTATAAG CAACACCAAG
    AAAAAGAAGA AAATCCTTAT GAGGGGTGAG AGTGGAGAGG TGACGGATGA TGAGATGGCC
    ACCCGCAAGG CCAGAACACA CAAAGAGAGC CGGAGCCGGA GTGGCTCTGA TCCTCAAGAT
    GTCAACGAAC AAGAAGAATC AGAGGCAAAT GCCATCATGA ACCCTCCCAA CCCTCTCCCT
    TCCAGAAGAG CCCACTCCTT GACCACGACT GGGCCCCCTA CCTTGGCTGC CGGGACAGCA
    TCTCCCATCA GGCCAGTGTC CTCTCCTGTG CTGCCTGCTT CCAACAGGAG CCCAACCAGT
    GCTTGGAACA GCAGCAGCGG CTGGCCAGGG AAGATCAAAG GAGGGATGCA GGGCTTTCAG
    AGCTTCATGG TTTCAGACAG CAGCATGAGT TTCGTGGAAT TTGTTGAGCT GTTCAAGTCA
    TTCAGTGTGA GGAGCCGCAA GGACCTGAAG GACCTCTTTG ACATCTACGC CGTACCCTGT
    GACCGAGCTG GCTCTGAGGC AGCCCCCCTC TATACCAACC TGACCATTGA CGAAAATATC
    AGCGGTCTTC AGCCTGACCT AGATTTGTTG ACCAGAAATG TCTCAGATTT GGGATTATTC
    ATCAAGAGTA AACAGCAGCT GTCTGACAAC CAGAGGCAGA TATCTGATGC CATTGCTGCT
    GCCAGTATTG TGACAAATGG CACCGGGGTG GAGAGCACAT CTCTGGGTGT GTTTGGAGTT
    GGTATCCTCC AGCTCAATGA CTTCCTAGTG AATTGCCAAG GAGAACACTA TACTTATGAT
    GAAATCCTCA GCATTATCCA GAAGTTCGAG CCTAGCGTTA GTATGTGTCA TCAAGGCCTA
    ATGTCATTTG AAGGATTTGC AAGGTTTCTA ATGGATAAAG ATAATTTTGC CTCGAAAAAT
    GATGAGTCAC AGGAGAACGT TAAAGAAATG CAGCTGCCCC TCTCCTACTA TTACATTGAA
    TCTTCACACA ATACCTACCT CACGGGCCAT CAGCTCAAAG GAGAATCCTC AGTAGAACTC
    TACAGCCAGG TCCTCCTGCA AGGCTGTAGG AGTGTAGAGT TGGACTGCTG GGACGGAGAT
    GATGGAATGC CCATCATTTA TCATGGACAC ACCCTGACAA CCAAGATCCC CTTCAAGGAA
    GTGGTTGAAG CCATCGATCG CAGTGCCTTC ATCAACTCTG ACCTACCCAT CATCATATCG
    ATTGAGAACC ACTGCTCATT GCCTCAGCAA CGAAAAATGG CAGAAATTTT CAAGACTGTG
    TTTGGAGAAA AACTGGTGGC TAAATTCTTA TTTGAGAGTG ATTTCTCAGA TGATCCTATG
    CTCCCCTCAC CTGACCAACT CAGAAGGAAA GTGCTTCTCA AAAATAAGAA ATTAAAAGCC
    CATCAGACAC CGGTGGATAT CTTAAAGCAA AAGGCTCATC AGCTAGCATC CATGCAAGCT
    CAGGCTTATA ATGGTAGCAA TGCCAACTCC CCACCTGCTA ATAATGAGGA AGAGGAAGAT
    GAAGAGGATG AATATGATTA TGATTATGAA TCCCTCTCTG ATGACAACAT TCTAGAAGAC
    AGACCTGAAA ATAAATCATG TAATGACAAA CTTCAGTTTG AGTATAATGA AGAAATCCCC
    AAGAGGATAA AGAAAGCAGA TAACTCTGCT TGCAACAAAG GGAAGGTTTA TGACATGGAA
    CTGGGAGAAG AATTTTATCT TCCTCAGAAT AAAAAGGAAA GCAGACAGAT TGCACCAGAG
    CTTTCTGACC TTGTCATCTA TTGCCAAGCA GTAAAATTCC CAGGCCTGTC AACTCTAAAT
    GCATCTGGCT CTAGCAGAGG AAAAGAAAGG AAAAGCAGGA AGTCCATTTT TGGCAACAAT
    CCGGGCAGAA TGAGCCCCGG GGAGACAGCA CCATTTAACA AAACATCTGG AAAAAGTTCC
    TGTGAAGGAA TGCGACAGGC CTGGGAGGAC TCTGCTTTCT CCGTCAACCC AACCACGTCC
    CTCAGCGCTA TCATTAGAAC TCCCAAATGC TACCATATCT CCTCACTGAA TGAAAATGCC
    GCCAAACGTC TGTGTCGCAG GTATTCTCAG AAACTCATCC AGCACACCGC CTGCCAGCTG
    CTGCGGACGT ACCCGGCTGC CACCCGCATC GACTCCTCCA ACCCCAACCC CCTCCTGTTC
    TGGCTCCATG GGATACAGCT CGTGGCGCTC AACTACCAGA CAGATGATCT CTCTTTACAT
    TTAAATGCCG CCATGTTTGA GGCCAATGGT GGCTGCGGTT ATGTTCTGAA ACCCCCAGTT
    CTGTGGGACA AGAACTGTTC CATGTATCAG AAGTTCTCCC CCTTGGAAAG AGACCTGGAC
    AGCATGGAGC CTGCCATCTA TTCTTTAACT ATTGTCTCTG GGCAGAACGT GTGCCCGAGT
    AACAGCACGG GGAGCCCATG TATCGAAGTC GATGTCCTGG GCATGGCCCT GGACAGCTGC
    CACTTCCGCA CGAAGCCCAT CCACCGCAAT ACTCTGAACC CCATGTGGAA CGAACAGTTT
    CTTTTCCGGG TTCACTTCGA AGATCTCGTA TTTCTTCGTT TTGCTGTCGT GGAGAACAAC
    AGCTCGGTGG TCACTGCTCA GAGAATCATC TCCCTCAAGG CTCTAAAGCG AGGATATCGA
    CATCTTCAGC TGAGAAATCT CCACAATGAA GTCTTGGAAA TCTCTAGTTT ATTCATAAAC
    AGCAGGAGGA TGGAAGAAAA TTCTTCCGGC AATACTGTGC TGGCCTCTCT GATGTTTAAT
    ACGGAAGAAA GAAAATGTTT GCAGACTCAC AGAGTCACAG TGCATGGCGT CCCAGGCCCA
    GAGCCCTTTA CTGTTTTCTC TATAAACGGA GGCACTAAGG CAAAGCAGCT TCTCCAACAA
    ATTCTCGTTA TGGATCAAGA TACCAAACCT ATTGCCACAG ATTATTTTTT AATGGAGGAA
    AAATATTTCA TATCTAAAGA GAAGAATGAA TGTAGGAAAC AGCCGTTCCA GAGAGCCATT
    GGTCCAGAAG AAGAGATCGT GCAGATTTTA AGCAGCTGGT TTCCAGAAGA GGGCTATGTT
    GGCAGGATTG TCTTAAAAAC CCAGCAGGAA AACCTGGAAG AGAAGAGCAT TGCACAAGAT
    GACAAGGAGA TGCTCCTGAG CTCAGAGGAG GACAGCTTCT TTGTCCAGGT GCATGACGTT
    TCCCCAGAGC AACCACGCAC GGTCATCAAG GCGCCGCGTG TCAGCACCGC GCAGGATGTC
    ATTCAGCAGA CCTTATGCAA AGCCAAATAT TCCTATAGCA TCCTGAGCAA CCCCAACCCG
    AGTGACTATG TGCTTTTGGA AGAGGTGGTG AAAGACACCG CCAACAGGAA ATCTTCCACC
    CCAAAGGCCT CCCAGCGCGT CCTTTTGGAT CAGGAGTGTG TGTTTCAAGC TCAAAGCAAG
    TGGAAAGGTG CAGGAAAATT CATCCTTAAG CTAAAGGAAC AGGTGCAGGC ATCTCGAGAA
    GATAAAAAAA AAGGCATCTC TTTTGCAAGC GAACTCAAGA AGCTCACCAA GACTAAACAG
    CCCCGAGGAC CCACATCACC TTCTCAGGTC TTGGCCTCAG AAAACGTCCA AAACAAGGAA
    GAGAAACCTG TGGGTGGCTT GCCTTCCAGT GACACCATAG ACTACCGACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225600.1
    CDS538..7431
    Translation

    Target ORF information:

    RefSeq Version XM_005225543.3
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase C epsilon 1 (PLCE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225543.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACTTCTG AAGAAATGGC AGGTTCTGTT CTCAGACCTG TGAGTCAGAG AAAAGTGATC 
    TCTGCTCAGT CAACTGTAGA TGAAAGTAAT GATAAGGTCT CGGGCATCAG TGTTCCGAAG
    TCTCATCCTG TCAGGCAGAG TGGGGAGACT TCTCATACCA TCTCGCAACC GACCAAACTT
    ACAGAAGAAT CTTCTGGAAG CAACGCGCCC CAGATTTTCT CAGTAGCAAG GGAGAAAATA
    ACCAGTGACG AGAACAGTAA TGAAAAGTGC TGGGAGAAAA GCATGCCAGA TTCTGTGAAA
    AACCTTAACA TTAACTGCAA CAGCATATTG AAAAACCATC AGCACGGCCC TCCTCAGAGC
    CAGTTTTATG AAACGTGCAA TTCTATCACA GAGGAAGGTC TGTGTTTGGA AACTGGAATC
    CCTTCTTCAC TGGAAGGAAA GGTGTTCCCT GGAATTCAAC TGGAAATAGA CACATCTCCC
    ATGGGCATGA GTCCATTAGG AACTCAGTCG GCCATCATAG AGACAAGCAG GGCACACCCT
    GAAAGCAACA TGGCAGTGTT TCACTTGCGT TACGAAGTTG ACAGAAGAAT GTCAGACAGT
    TTCTGTCCCC TGTCAGATAA CTTGATTTTG GATGATTGTG GAAACTGTGT ACTACCTGCC
    GTTGGTGAGG AACAAGAGAA AAATTACATG GCATATACTT GCAAACTGAT GGAATTGACA
    AATAACTGTG ACAATAAGAA TAGGCAGCTG CAGTGTGATC ATTGTGACCC CTTGAATGAC
    AAATACTTGT GCTTTGAAGG CTCTTGCCCA AAGGCCGATG TGGTATGTTC AAGTGACAGC
    TTTTGCAGGG AGGATTTTAC TGACAGTCCA CCTGCCAAGA CCTTTCTGAG CCATTTTGAG
    GACTTCCCCG ATAATTGTGA AGAGGTAGAA GAAGATTTGT TCAAAAATAA AAAGGAGCGG
    TCTGCTTGGT TAGTGAGGAG ATTTTGCAAA AATGACAAGG AAGTTAAGAA ATCTGTGTTT
    ACTGGGACAA GGGCAATTGT GAGAACTTTG CCTTCTGGTC ACATTGGACT GGAGGCTTTG
    AGTTACATTG ATCAGAAGAA AAATGGTCTC TTATTGCCTC CTGGGAGAGT CATGGAACGG
    CTGTCAACAA TGGTGGTTAG GCAAGATGGT TCCCAAGGTC TGTCAGAAGC CCAGTGGTAT
    CCGATCTACA GTGCAGTGAG AAGGGGAGAA GCCACAGAAC AGACAATTAG ATCTCTTCTC
    CATTTCTTCG CCAAGCTCCC AGCCTCCGAG ACAGCCCATG AGAGGATCAG TGTCGGTCCG
    TACTTAAAGC AATGTGTCCG AGACACAGTG TGTGAGTATC GTGCCACCTT GCAAAGGACT
    TCCATATCTC AGTATATCAC CGGCTCTCTC CTGGAAGCCA CCACATCTTT AGGAGCAAGA
    AGCAGCCTCC TCAATTCTTT TGGAGGATCC ACGGGACGAA TGATGCTGAA AGAACGCCAA
    CCAGGCACCT CTATGGCCAA CTCCAGTGCC CTTCCTTCAA GTTCAGCTGG GATCAGCAAG
    GAACTGATTG ATCTGAAGCC CCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG
    GAGCAGGAGC AAAATATTTA CCGAAGGGTC TTGCCAGTTG ACTATCTTTT CTTCTTGACC
    CGGGGCTTGG GCACAACAGA ACGCCAGAGG TCCCTGCCCT GCCTCAAAGC ATCCATCTCA
    GCATCCATTC TTACCTCCCA GAAGGGAGAG CACAATGCCC TCGAAGATCT TGTGATGAGA
    TTTAATGAGG TGAGCTCCTG GGTGACGTGG CTGATCCTCG CGGCGGGCTC CATGGAAGAG
    AAGCGGGAAG TCTTCTCATA CTTGGTTCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC
    TACAATGCCG TCATGGAGTT CTTGGCCGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG
    CAGTTTATGG ACCAGTCTGA TCTGGAGACC ATGAGGAGCC TGAAGGACGC CATGGCCCAG
    CACGAGTCTT CCTGCGAGTA CAGGAAGGTG GTGACCCGAG CGCTGCACAT CCCCGGCTGC
    AAGGTGGTTC CCTTCTGCGG GGTGTTTCTG AAAGAGCTCT GTGAGGTGCT GGACGGAGCC
    TCTGGCCTCA TGAAGCTTTG CCCGAGGTAC AATTCGCAGG AAGAAACTCT GGAGTTTGTA
    GCCGATTATA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAGAATGG CTTAAAGAAT
    TCAGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGATCA TCAGGAGCTG CAGTCGAAGT
    CTGGAGACAG AGGAGGAGGA CAGCTCCAGT GAAGGGAGCA GCTCAAGGAA AAATTCCCTG
    AAGGATAAAG CCCGATGGCA GTTTATAATC GGAGATTTGT TGGATTCTGA AAATGACATC
    TTTGAGCAGC CCAAAGAGTG GGACTCTCAT GGATCGGAGG AGCCACAGAA AGCCTTTGAC
    CATGGGACAG AGCTCATCCC ATGGTATGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC
    CTGTTGCAGG GGGCCACGGT CATCCACTAT GATCAGGACA CGCACCTCTC TGCCCGCTGC
    TTCCTCCAGC TTCAGCCTGA CAATAGCACC TTGACCTGGA TAAAACCCAC CACTGCTTCC
    CCAGCCAGTG CTAAAGCAAA ACTTGGTGTG CTCAGTAACA CGTCTGAGCC TGGCAAATTC
    CCGTTACTGG GCAATGCTGG ATTAAGTGGC CTGGTGGAGG GGATCTTGGA TCTGTTTTCA
    GCAAAGGCTG TGTACATGGG ACACCCTAAC ATTGATATAC ACACTGTGTG TGTTCAGAAC
    AAACTGAGTA ACATGTCTCT CTCAGAGACT GGCGTGACAC TGCTCTACGG GCTGCAGACC
    ACGGACAATA GATTACTGCA CTTTGTGGCG CCAAAGCACA CAGCTAAAAT GCTCTTCAGT
    GGATTGTTGG AGCTCACGAG AGCTGTGAGG AAGATGAGGA AGTTCCCTGA CCAAAGACAG
    CATTGGCTGC GGAAACAGTA CGTCAGCCTC TACCAGGAGG ATGGACGATA TGAAGGCCCA
    ACTTTGGCTC AAGCTGTAGA GCTGTTTGGT GGCAGACGAT GGAGTACTCG AAACCCCAGC
    CCTGGGACAT CAGCAAAGAG CGCTGAGAAG CCCAACACCT TGAGTATAAG CAACACCAAG
    AAAAAGAAGA AAATCCTTAT GAGGGGTGAG AGTGGAGAGG TGACGGATGA TGAGATGGCC
    ACCCGCAAGG CCAGAACACA CAAAGAGAGC CGGAGCCGGA GTGGCTCTGA TCCTCAAGAT
    GTCAACGAAC AAGAAGAATC AGAGGCAAAT GCCATCATGA ACCCTCCCAA CCCTCTCCCT
    TCCAGAAGAG CCCACTCCTT GACCACGACT GGGCCCCCTA CCTTGGCTGC CGGGACAGCA
    TCTCCCATCA GGCCAGTGTC CTCTCCTGTG CTGCCTGCTT CCAACAGGAG CCCAACCAGT
    GCTTGGAACA GCAGCAGCGG CTGGCCAGGG AAGATCAAAG GAGGGATGCA GGGCTTTCAG
    AGCTTCATGG TTTCAGACAG CAGCATGAGT TTCGTGGAAT TTGTTGAGCT GTTCAAGTCA
    TTCAGTGTGA GGAGCCGCAA GGACCTGAAG GACCTCTTTG ACATCTACGC CGTACCCTGT
    GACCGAGCTG GCTCTGAGGC AGCCCCCCTC TATACCAACC TGACCATTGA CGAAAATATC
    AGCGGTCTTC AGCCTGACCT AGATTTGTTG ACCAGAAATG TCTCAGATTT GGGATTATTC
    ATCAAGAGTA AACAGCAGCT GTCTGACAAC CAGAGGCAGA TATCTGATGC CATTGCTGCT
    GCCAGTATTG TGACAAATGG CACCGGGGTG GAGAGCACAT CTCTGGGTGT GTTTGGAGTT
    GGTATCCTCC AGCTCAATGA CTTCCTAGTG AATTGCCAAG GAGAACACTA TACTTATGAT
    GAAATCCTCA GCATTATCCA GAAGTTCGAG CCTAGCGTTA GTATGTGTCA TCAAGGCCTA
    ATGTCATTTG AAGGATTTGC AAGGTTTCTA ATGGATAAAG ATAATTTTGC CTCGAAAAAT
    GATGAGTCAC AGGAGAACGT TAAAGAAATG CAGCTGCCCC TCTCCTACTA TTACATTGAA
    TCTTCACACA ATACCTACCT CACGGGCCAT CAGCTCAAAG GAGAATCCTC AGTAGAACTC
    TACAGCCAGG TCCTCCTGCA AGGCTGTAGG AGTGTAGAGT TGGACTGCTG GGACGGAGAT
    GATGGAATGC CCATCATTTA TCATGGACAC ACCCTGACAA CCAAGATCCC CTTCAAGGAA
    GTGGTTGAAG CCATCGATCG CAGTGCCTTC ATCAACTCTG ACCTACCCAT CATCATATCG
    ATTGAGAACC ACTGCTCATT GCCTCAGCAA CGAAAAATGG CAGAAATTTT CAAGACTGTG
    TTTGGAGAAA AACTGGTGGC TAAATTCTTA TTTGAGAGTG ATTTCTCAGA TGATCCTATG
    CTCCCCTCAC CTGACCAACT CAGAAGGAAA GTGCTTCTCA AAAATAAGAA ATTAAAAGCC
    CATCAGACAC CGGTGGATAT CTTAAAGCAA AAGGCTCATC AGCTAGCATC CATGCAAGCT
    CAGGCTTATA ATGGTAGCAA TGCCAACTCC CCACCTGCTA ATAATGAGGA AGAGGAAGAT
    GAAGAGGATG AATATGATTA TGATTATGAA TCCCTCTCTG ATGACAACAT TCTAGAAGAC
    AGACCTGAAA ATAAATCATG TAATGACAAA CTTCAGTTTG AGTATAATGA AGAAATCCCC
    AAGAGGATAA AGAAAGCAGA TAACTCTGCT TGCAACAAAG GGAAGGTTTA TGACATGGAA
    CTGGGAGAAG AATTTTATCT TCCTCAGAAT AAAAAGGAAA GCAGACAGAT TGCACCAGAG
    CTTTCTGACC TTGTCATCTA TTGCCAAGCA GTAAAATTCC CAGGCCTGTC AACTCTAAAT
    GCATCTGGCT CTAGCAGAGG AAAAGAAAGG AAAAGCAGGA AGTCCATTTT TGGCAACAAT
    CCGGGCAGAA TGAGCCCCGG GGAGACAGCA CCATTTAACA AAACATCTGG AAAAAGTTCC
    TGTGAAGGAA TGCGACAGGC CTGGGAGGAC TCTGCTTTCT CCGTCAACCC AACCACGTCC
    CTCAGCGCTA TCATTAGAAC TCCCAAATGC TACCATATCT CCTCACTGAA TGAAAATGCC
    GCCAAACGTC TGTGTCGCAG GTATTCTCAG AAACTCATCC AGCACACCGC CTGCCAGCTG
    CTGCGGACGT ACCCGGCTGC CACCCGCATC GACTCCTCCA ACCCCAACCC CCTCCTGTTC
    TGGCTCCATG GGATACAGCT CGTGGCGCTC AACTACCAGA CAGATGATCT CTCTTTACAT
    TTAAATGCCG CCATGTTTGA GGCCAATGGT GGCTGCGGTT ATGTTCTGAA ACCCCCAGTT
    CTGTGGGACA AGAACTGTTC CATGTATCAG AAGTTCTCCC CCTTGGAAAG AGACCTGGAC
    AGCATGGAGC CTGCCATCTA TTCTTTAACT ATTGTCTCTG GGCAGAACGT GTGCCCGAGT
    AACAGCACGG GGAGCCCATG TATCGAAGTC GATGTCCTGG GCATGGCCCT GGACAGCTGC
    CACTTCCGCA CGAAGCCCAT CCACCGCAAT ACTCTGAACC CCATGTGGAA CGAACAGTTT
    CTTTTCCGGG TTCACTTCGA AGATCTCGTA TTTCTTCGTT TTGCTGTCGT GGAGAACAAC
    AGCTCGGTGG TCACTGCTCA GAGAATCATC TCCCTCAAGG CTCTAAAGCG AGGATATCGA
    CATCTTCAGC TGAGAAATCT CCACAATGAA GTCTTGGAAA TCTCTAGTTT ATTCATAAAC
    AGCAGGAGGA TGGAAGAAAA TTCTTCCGGC AATACTGTGC TGGCCTCTCT GATGTTTAAT
    ACGGAAGAAA GAAAATGTTT GCAGACTCAC AGAGTCACAG TGCATGGCGT CCCAGGCCCA
    GAGCCCTTTA CTGTTTTCTC TATAAACGGA GGCACTAAGG CAAAGCAGCT TCTCCAACAA
    ATTCTCGTTA TGGATCAAGA TACCAAACCT ATTGCCACAG ATTATTTTTT AATGGAGGAA
    AAATATTTCA TATCTAAAGA GAAGAATGAA TGTAGGAAAC AGCCGTTCCA GAGAGCCATT
    GGTCCAGAAG AAGAGATCGT GCAGATTTTA AGCAGCTGGT TTCCAGAAGA GGGCTATGTT
    GGCAGGATTG TCTTAAAAAC CCAGCAGGAA AACCTGGAAG AGAAGAGCAT TGCACAAGAT
    GACAAGGAGA TGCTCCTGAG CTCAGAGGAG GACAGCTTCT TTGTCCAGGT GCATGACGTT
    TCCCCAGAGC AACCACGCAC GGTCATCAAG GCGCCGCGTG TCAGCACCGC GCAGGATGTC
    ATTCAGCAGA CCTTATGCAA AGCCAAATAT TCCTATAGCA TCCTGAGCAA CCCCAACCCG
    AGTGACTATG TGCTTTTGGA AGAGGTGGTG AAAGACACCG CCAACAGGAA ATCTTCCACC
    CCAAAGGCCT CCCAGCGCGT CCTTTTGGAT CAGGAGTGTG TGTTTCAAGC TCAAAGCAAG
    TGGAAAGGTG CAGGAAAATT CATCCTTAAG CTAAAGGAAC AGGTGCAGGC ATCTCGAGAA
    GATAAAAAAA AAGGCATCTC TTTTGCAAGC GAACTCAAGA AGCTCACCAA GACTAAACAG
    CCCCGAGGAC CCACATCACC TTCTCAGGTC TTGGCCTCAG AAAACGTCCA AAACAAGGAA
    GAGAAACCTG TGGGTGGCTT GCCTTCCAGT GACACCATAG ACTACCGACA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.