plc-1 gene cDNA ORF clone, Caenorhabditis elegans

mRNA	Protein	Name
NM_001029448.4	NP_001024619.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
NM_077404.6	NP_509805.3	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
NM_001373493.1	NP_001360636.1	PhosphoLipase C
NM_001129669.2	NP_001123141.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
NM_001373491.1	NP_001360639.1	PhosphoLipase C
NM_077404.5	NP_509805.3	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
NM_001136454.1	NP_001129926.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
NM_001029446.3	NP_001024617.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
NM_001136454.2	NP_001129926.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
NM_001129669.3	NP_001123141.2	PhosphoLipase C
NM_001373489.1	NP_001360637.1	PhosphoLipase C
NM_001373494.1	NP_001359720.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
NM_001136455.3	NP_001129927.1	Phosphoinositide phospholipase C
NM_001373495.1	NP_001360638.1	PhosphoLipase C
NM_001029448.3	NP_001024619.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
NM_001029446.2	NP_001024617.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
NM_001136455.2	NP_001129927.1	Phosphoinositide phospholipase C
NM_001373492.1	NP_001360635.1	PhosphoLipase C

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa348502	NM_001029446.3 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa348503	NM_077404.6 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa348940	NM_001129669.2 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa348985	NM_001029448.4 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa349046	NM_001136454.2 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa349047	NM_001136455.3 Latest version!	Caenorhabditis elegans Phosphoinositide phospholipase C (plc-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa851220	NM_001373494.1 Latest version!	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCa851221	NM_001129669.3 Latest version!	Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCa851222	NM_001373492.1 Latest version!	Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCa851223	NM_001373493.1 Latest version!	Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCa851224	NM_001373489.1 Latest version!	Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCa851225	NM_001373495.1 Latest version!	Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCa851226	NM_001373491.1 Latest version!	Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OCa348503	NM_077404.5	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa349046	NM_001136454.1	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa348985	NM_001029448.3	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa348502	NM_001029446.2	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCa349047	NM_001136455.2	Caenorhabditis elegans Phosphoinositide phospholipase C (plc-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

plc-1 ( NM_001029446.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024617.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), mRNA.

Price & Availability↑

CloneID	OCa348502
Clone ID Related Accession (Same CDS sequence)	NM_001029446.3 , NM_001029446.2
Accession Version	NM_001029446.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_001029446.2.

ATGAACTGGG ACACTCTGAA AGGCGTGCTG AAAACAAGGC GTTTGACAAA ACGTACGATT 
CCAGCGTATA TTCATCCAAC ATCACGTAGT GACTCAACCT CTTCAACGCA ATCAGCCACA 
GCTGGATTCA TTCTCAATGA AGAACCAATC ACACTGTTCC GACTTGAACT CGAACGACTT 
CAATATATTC TTCATTTCCC TGAAGAAGTT GCATTCCAAC TATCTTCTAC TGAGTATCAG 
CTTTTCTACT CAATACAACC CATGGATTAT GTTCGTTACG TCAGTTGCGA TCTCACATCT 
GTTCCAGTGT CAGAAAATCC AAGTCCAGTG CGAAATCTTG TGAAAAGGCT ATCAGAGGTA 
TCATCCTGGA TAACACATGT TATTGTCAGT CAGCCAACAC ATGACGATCG AAAGGTTGCT 
CTTACTGCAA TTTTGAGAAT TGTGGAAACT TGCTGGAATA TTGGAAACTT TAATGCAGCT 
GTTGAGGTTC TTATGGGTTT AAAGAGTGAA AAACTCAGAC CATTCTGGCT TTCTTTGCGA 
CAAGAGGAAA AGTCTCAGTT TGATTCGCTT TGCGAAACCT TGCTTCCTGC AAACCAAGCT 
CTCCCATCTC AAGCTTACAT CAACGCGGTC CAACGAGCAC TGAGAATGCC ACAATCTCGT 
GTGATTCCAT TTTTTGGAAT ATTTCTTCGT GATCTCTACG CAATTGTGAA TGACTTGCCG 
AATATCGTTG TGATTGGACA AGAAGGTGAA ACCCAAAAGC TGGAATTCAT GAGCGATCCC 
AATGGCGAAG ATCATTTTTC GTCGAGAATT GGAGTGGGTG GGCTGTTGAA TGCTGATAAA 
ATCAACTTGG TGGCAATTGT TTTGGACAAC TTGGAATTAT TCCATCGTCA CAGCAGGACA 
ATGATCAAAT TGTTAGAAGA GCAAGCAGTC CCACCAATTC AAATTCCTCA GAACGAGCGG 
GAGCAAAAAG AAAAGGAAGC AAAAACATAT GAACCAGTAC AAGTGGTTCG CGGCTCTTCT 
CATGGAGTGG CTCTCATCCC TCTTGATACC CTAACATTCG ATCTTGATGT TATCCAACGA 
CTCCAGCACG GAACAACAGT GATCCATTAC GAACCTGATT CGGGAAGAAG CAATTTGTGT 
CTGCTGCGGT TAGACCCTAG TTGCGGTCAA ATAAACTGGC ATAAGATTTC ATATTCAGTT 
AACAAAGATC CCAAAGAAAA GGATGTGCTC GCCAAAGTAT CCGTTTCAAA TCTGCAGCCG 
CTAGACTCTG GCCGAGGGGC TCCGTCACCA ATGCCATCTG GAAGAACACC AGGAACTGGA 
GGAGTTGGAG TAGAGGAGGG AGAACTCAAA TTAAGTGTTG TGAAAGGAGT CGAACTAGTT 
GATAGTTATG ATATTGATAT TGAGGCAATT TACCGTCGTC ATTCTATGGA AGAGATGTCA 
GTTCCTGTTA GCTGTTGGAA AGTTAGTCAT GGTCAGTTGT TATCGGATAA TGAGTTCATC 
TACTTTTTGG CCCCTCAACA AATTGCTCAA TTTTGGACCA ACGGGTTGCA ATCTGTAGTT 
AAAAGTCTCC AAGGACAGCA AAGGTACCCT GATCGGCGAA TGTTATGGAT CAAAAATGTC 
TACTTATCAC TTTACGAGAT AACGGGCGAA TCAAACTGTG GACCACGGCC ATTTGAAGCA 
CTTCAGGCAT TTGGGCTGAG TCAAACAAAT ACTAACGCAA CACGACCCAA TGACTCGTCG 
CTGTCCAGTG AGCCAGGAGG AGCTAAAAGC AGACTGAAGA ACCTTAAAAA TGCTATGCAA 
AAGAAGCTTC GTGGTGCATC GCGCGAAGGA AGCAGATCTC AGTCACCTCA ACCTCATTCT 
CCTCTTGTCC GACCACCATC AATAAAATCG CAAATATCGT CACAATCTGG TCCGCCGGGT 
CCGAACTCTC CAGGCTATTT GCTGAAACCA CGTGGAGAAC CTGCCAATAG TGATGCAGGA 
GACATTGATA GCATTTATAC ACCTCGATCA AGAACTCCAA CAAGCAGCTC CTATGGAGGG 
CGATCCGTTG GAGGGAGATC ATGTAAATCG TGGAGATCAA GAGGAGGAGA GACACCAAAT 
TCGGGTTCCA TAAGCAGCTC GGGTCAGATG TCAATTCAAG TGTCTGGTCT GAGTGGACCA 
TCGGGAAAAG AGTTTCAGGA AAAACCATTA ACACTTGTAG AGTTCGCTGA ACTTTTTCGA 
TTGTTCAATA CAAGAATGAG AAAAGATTTG AGAGATGTGT TCAATGATGT CCTGTCTACG 
GCAACTACTC CTCAGCATTG TCCTAAAAGA GAACGTGATC GCCACAGTCC GAGAATGCAG 
TCAAGGCTGG CGAGTGTCTC CAACTCGTAC AATGCAGATT TTCTGTCTAA CGATTTCTTG 
ACTCGGAATA CGGCAGTAAC GTCCCATCAT ATCAGTGAAA AGCAAAACAA AATCTACAAT 
GCATTGGCAC TTGCGAGTGT TAATAGCATG GGCGGACTCA TGGACACTTC CCGGTCGTCG 
ATGCTGACGC CGCAAATGCT GCGCGCTTTT GTCAACACTC ATCAGATGGA GCAGATTGAC 
GAGCAAACGG CGATTAAGCT TATTCAGGAT CATGAACCTG ATGGTATCTG TCGACAAAAG 
AATCAAATGT CATTCGAAGG ATTCACTAGA TTCCTTTGCG ATCCTGTCAA CTTTGCATTC 
GTACCGGAAA CAATTGAACC AGATGAAGAG GACCTACGGT ACCCGTTGTC TCATTATTAC 
ATCAATTCAA GTCACAACAC CTATTTGACG GGACATCAAC TCAAGGGACC CAGTAGCTCG 
GAGATGTACA GACAGGTTCT CCTCACCGGG TGTCGGTGTG TCGAGCTTGA TTGTTGGGAT 
GGAGATGACG GATTGCCATT GATTTATCAT GGACACACAT TGGTTAGCAA GATTGGGTTT 
AGACAGGTGG TCGAAATTAT CAAAAAATCT GCATTCATAA CATCGGATCT TCCTGTGATA 
TTATCAATTG AGAATCATTG TTCCCTACAA CAACAGGCGA AAATGGCACA GATGTTCAAA 
ACAGTTCTTG GAGATTTACT AGTATCAAAC TTCCTTTTTG AAGCTGATTT CTCGGACTCT 
CCACGTCTTC CATGCCCTCT CCAAATGAAA AATAAGATCT TGATAAAAAA TAAGAAAATG 
ATAGTCGACC CACCAACTCC ATTGCCAATG ATTGAACGAG GAGCTGTGCA GCGTGGTGAA 
ACTCAATTAA ATCTTCACAG AAAACAGAGT AAAAACTCCT ACGAAAGCAG TACGGTAGAT 
GAAGTGGAAG ATGACGACCT AGATGAGTTT CTTGACGATG AAGAAAATGA GGAGGATGAC 
CAAGAAGAGG TTCAAGTCAG AAGTGAAAAA GAAGACAGTC CGAAGACATC AAAACGTGCA 
GAGAAATCGG CTAGAAACAT CAAACAGCAA GATTCGTTAT GTAGCGATCA TTCGGTAGAG 
CAGGCAAAAC CTTCCACAAG CAAGACCACA TCCAAAACCA ACGATAGAAA GACTGAAGAT 
GAAGTACTTT ACGCTCAACT TGCTCAAAAT GCCATCCGAA ACCAACAACC AAGAAAGAAC 
AATACTGGAG TCCAAATAGC GCCAGAGTTG TCAGATATTG TCATTTACAT GCAAGCGACA 
AAATTCAAAG GATTTCCCCC AGTCGATGGC ATTCAATCAC CAAGGATAAT GGAGGAAGGG 
CCAGCTTCTG CAAGTCTTTC ATTTTCATCA CGGGCTCGTA CGCCATCAAA TCTGCTGAAT 
ACACCAGCCC CTCCACGACG ACAGCGAAGT AGCACTCAGT TGAGTCAAGA ACTGGCTGCA 
GAATTTTTGG GATCAGTTAG GGCAAATGCT ACAGCTACAT GCTACCAGGT TACATCTCTT 
AATGAAAACG CTGCAAAGAA ACTGATGAAA AGACATCCAG CAAAATGCGT ATCTTATACT 
CGGGACCATC TGATTCGTAC GTATCCAAGT GCCAAGCACT ATGACTCGTC CAACTTTAAC 
CCCATCAACT GCTGGGCTCA TGGAATGCAA ATGGTTGCTT TAAATTTTCA AACTCCTGAT 
GTTATAATGG CAGTTAACCA AGCGATGTTC GAGCAATCTG GAAACTGTGG ATATCAACTG 
AAACCACGTT GTCTTTGGGA TGAATCTCAT CTTTTGTACA ACAAATTTTT GCCTCTCTCC 
AAAGACATTG CCGGCCATTC TGCTTTGTTA CTAAACTTAA CTATTATATC CGGACAGCAC 
GTGTATCCAA ACACCCATTA CGCCAGTTTA TATGTCGAAA TTGAAGTCAT CGGTATTCAC 
AACGACTGTG TTCGTGAGAA ATCAAAAGTG GTCCAGAGAA ATTCTGTGAA TCCAATCTGG 
AACCACACCA CTCAACTTCG AATAGCTTGT GTTGACTTAG CATTCCTTCG CATAGCAGTA 
TGTGATAGTG GGCAAAATGG AAGAGTCGTT GCTCACCGAG TTGTTCCTGT CAAATGCATT 
CGTCCGGGTT TCCGACATCT CCCACTCAGA ACTCCAACTA ATCTTCCGAT CGACAACGCT 
ATGATATTTT TAAGAACAAG ATTCGAGCAA GAAGAACACA TCTACCTCCA CGACGATGAT 
TCAAACACCT ATTGTAATCT AGAACATACG CTGGCATACC GCACAGACTT AACACCCAAT 
TTATCTCCAA CGCCAATTTT GAAGAAACAG ATCTTTGTTC TTCGAATTAC AGGCGCGTTT 
GCTGATGAAA CAGCAATTAC AGTGCACAGC GAGTCAGGAA GTACAGTGAA GACGGTGATG 
CAACAGGCGC TGCTTAACGC TGGAAAGAAT GCGGATCAGG TTGAAGAGTA TGTACTCATC 
GAAGAATCAC TGCCGGCACC TTCTGGGGAA GATCCAATTG AGCAGAGAGT ACTTCCTCTG 
AACGAGCCTA TCATGGACGC CGTGGCCTGC TGGAACGGTT CAATGCGTCG TTTTGTGTTA 
CGAAAAAAAG GCAGTGACCC ATCATCTAGA GCTTGGATCA CTTCAATTAT AAAATCCGGA 
ACAAGTGGAA GCTCCACGTC AGTCTCACCA AGTCCGTTGA CCAAAGATGG ACACGTAAAG 
AGTGCATCAA GCAATCAACT TCATGGACGA TCATTGGACA CTGACGCTTT TGGAGAGCAC 
CTTGAAGTGA CAGAAGGCAA GTGGTTGAAC CCTCGTGCAA GATCAATGGG CGACACATTT 
CTGGTATGTG TTCACAATGT TAGTGAAGAT CAGCCTTATG CAATCCTACG TGCTGGAATC 
CACAGCACTG CCGCCGACAT TATTCGTCAG GTGTTTGTTA AAGCTCGCCG TTCAAATGTA 
GACGATTCCG AGTTTGTTCT TGTTGAAGAA ACCTGCGATG ATCCAAAACT CAATCAAGGC 
CAAAGTATGC TACAAGCCCT TTCGTTGGCG CGGAAAAGAA GCAATGATCT GACTCCAAAG 
TATCCAAACA ACCGCACCAC AAGCCGAGTG TTAGGACAAA ACGAAAATGT TTGGAAAGCA 
CAATCGAGGT GGAAGTCAAT GGGCAGATTT GTACTTGAAA ACCGAAAGGA CACAGTGCAC 
GCAACCCTCG AAAAAGAGTT TCACGAAATG GCAAAAATCA TTCGTGAGGG TATTCCAAAA 
AAGGACGAAA CTTATTATAT GATATACTAC TCAGGACTGC CAGGAGAGGA TATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024617.1
CDS	39..5735
Translation	MNWDTLKGVLKTRRLTKRTIPAYIHPTSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGKWLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQSMLQALSLARKRSNDLTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKEFHEMAKIIREGIPKKDETYYMIYYSGLPGEDI

Target ORF information:

RefSeq Version	NM_001029446.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029446.3

ORF Insert Sequence:

ATGAACTGGG ACACTCTGAA AGGCGTGCTG AAAACAAGGC GTTTGACAAA ACGTACGATT 
CCAGCGTATA TTCATCCAAC ATCACGTAGT GACTCAACCT CTTCAACGCA ATCAGCCACA 
GCTGGATTCA TTCTCAATGA AGAACCAATC ACACTGTTCC GACTTGAACT CGAACGACTT 
CAATATATTC TTCATTTCCC TGAAGAAGTT GCATTCCAAC TATCTTCTAC TGAGTATCAG 
CTTTTCTACT CAATACAACC CATGGATTAT GTTCGTTACG TCAGTTGCGA TCTCACATCT 
GTTCCAGTGT CAGAAAATCC AAGTCCAGTG CGAAATCTTG TGAAAAGGCT ATCAGAGGTA 
TCATCCTGGA TAACACATGT TATTGTCAGT CAGCCAACAC ATGACGATCG AAAGGTTGCT 
CTTACTGCAA TTTTGAGAAT TGTGGAAACT TGCTGGAATA TTGGAAACTT TAATGCAGCT 
GTTGAGGTTC TTATGGGTTT AAAGAGTGAA AAACTCAGAC CATTCTGGCT TTCTTTGCGA 
CAAGAGGAAA AGTCTCAGTT TGATTCGCTT TGCGAAACCT TGCTTCCTGC AAACCAAGCT 
CTCCCATCTC AAGCTTACAT CAACGCGGTC CAACGAGCAC TGAGAATGCC ACAATCTCGT 
GTGATTCCAT TTTTTGGAAT ATTTCTTCGT GATCTCTACG CAATTGTGAA TGACTTGCCG 
AATATCGTTG TGATTGGACA AGAAGGTGAA ACCCAAAAGC TGGAATTCAT GAGCGATCCC 
AATGGCGAAG ATCATTTTTC GTCGAGAATT GGAGTGGGTG GGCTGTTGAA TGCTGATAAA 
ATCAACTTGG TGGCAATTGT TTTGGACAAC TTGGAATTAT TCCATCGTCA CAGCAGGACA 
ATGATCAAAT TGTTAGAAGA GCAAGCAGTC CCACCAATTC AAATTCCTCA GAACGAGCGG 
GAGCAAAAAG AAAAGGAAGC AAAAACATAT GAACCAGTAC AAGTGGTTCG CGGCTCTTCT 
CATGGAGTGG CTCTCATCCC TCTTGATACC CTAACATTCG ATCTTGATGT TATCCAACGA 
CTCCAGCACG GAACAACAGT GATCCATTAC GAACCTGATT CGGGAAGAAG CAATTTGTGT 
CTGCTGCGGT TAGACCCTAG TTGCGGTCAA ATAAACTGGC ATAAGATTTC ATATTCAGTT 
AACAAAGATC CCAAAGAAAA GGATGTGCTC GCCAAAGTAT CCGTTTCAAA TCTGCAGCCG 
CTAGACTCTG GCCGAGGGGC TCCGTCACCA ATGCCATCTG GAAGAACACC AGGAACTGGA 
GGAGTTGGAG TAGAGGAGGG AGAACTCAAA TTAAGTGTTG TGAAAGGAGT CGAACTAGTT 
GATAGTTATG ATATTGATAT TGAGGCAATT TACCGTCGTC ATTCTATGGA AGAGATGTCA 
GTTCCTGTTA GCTGTTGGAA AGTTAGTCAT GGTCAGTTGT TATCGGATAA TGAGTTCATC 
TACTTTTTGG CCCCTCAACA AATTGCTCAA TTTTGGACCA ACGGGTTGCA ATCTGTAGTT 
AAAAGTCTCC AAGGACAGCA AAGGTACCCT GATCGGCGAA TGTTATGGAT CAAAAATGTC 
TACTTATCAC TTTACGAGAT AACGGGCGAA TCAAACTGTG GACCACGGCC ATTTGAAGCA 
CTTCAGGCAT TTGGGCTGAG TCAAACAAAT ACTAACGCAA CACGACCCAA TGACTCGTCG 
CTGTCCAGTG AGCCAGGAGG AGCTAAAAGC AGACTGAAGA ACCTTAAAAA TGCTATGCAA 
AAGAAGCTTC GTGGTGCATC GCGCGAAGGA AGCAGATCTC AGTCACCTCA ACCTCATTCT 
CCTCTTGTCC GACCACCATC AATAAAATCG CAAATATCGT CACAATCTGG TCCGCCGGGT 
CCGAACTCTC CAGGCTATTT GCTGAAACCA CGTGGAGAAC CTGCCAATAG TGATGCAGGA 
GACATTGATA GCATTTATAC ACCTCGATCA AGAACTCCAA CAAGCAGCTC CTATGGAGGG 
CGATCCGTTG GAGGGAGATC ATGTAAATCG TGGAGATCAA GAGGAGGAGA GACACCAAAT 
TCGGGTTCCA TAAGCAGCTC GGGTCAGATG TCAATTCAAG TGTCTGGTCT GAGTGGACCA 
TCGGGAAAAG AGTTTCAGGA AAAACCATTA ACACTTGTAG AGTTCGCTGA ACTTTTTCGA 
TTGTTCAATA CAAGAATGAG AAAAGATTTG AGAGATGTGT TCAATGATGT CCTGTCTACG 
GCAACTACTC CTCAGCATTG TCCTAAAAGA GAACGTGATC GCCACAGTCC GAGAATGCAG 
TCAAGGCTGG CGAGTGTCTC CAACTCGTAC AATGCAGATT TTCTGTCTAA CGATTTCTTG 
ACTCGGAATA CGGCAGTAAC GTCCCATCAT ATCAGTGAAA AGCAAAACAA AATCTACAAT 
GCATTGGCAC TTGCGAGTGT TAATAGCATG GGCGGACTCA TGGACACTTC CCGGTCGTCG 
ATGCTGACGC CGCAAATGCT GCGCGCTTTT GTCAACACTC ATCAGATGGA GCAGATTGAC 
GAGCAAACGG CGATTAAGCT TATTCAGGAT CATGAACCTG ATGGTATCTG TCGACAAAAG 
AATCAAATGT CATTCGAAGG ATTCACTAGA TTCCTTTGCG ATCCTGTCAA CTTTGCATTC 
GTACCGGAAA CAATTGAACC AGATGAAGAG GACCTACGGT ACCCGTTGTC TCATTATTAC 
ATCAATTCAA GTCACAACAC CTATTTGACG GGACATCAAC TCAAGGGACC CAGTAGCTCG 
GAGATGTACA GACAGGTTCT CCTCACCGGG TGTCGGTGTG TCGAGCTTGA TTGTTGGGAT 
GGAGATGACG GATTGCCATT GATTTATCAT GGACACACAT TGGTTAGCAA GATTGGGTTT 
AGACAGGTGG TCGAAATTAT CAAAAAATCT GCATTCATAA CATCGGATCT TCCTGTGATA 
TTATCAATTG AGAATCATTG TTCCCTACAA CAACAGGCGA AAATGGCACA GATGTTCAAA 
ACAGTTCTTG GAGATTTACT AGTATCAAAC TTCCTTTTTG AAGCTGATTT CTCGGACTCT 
CCACGTCTTC CATGCCCTCT CCAAATGAAA AATAAGATCT TGATAAAAAA TAAGAAAATG 
ATAGTCGACC CACCAACTCC ATTGCCAATG ATTGAACGAG GAGCTGTGCA GCGTGGTGAA 
ACTCAATTAA ATCTTCACAG AAAACAGAGT AAAAACTCCT ACGAAAGCAG TACGGTAGAT 
GAAGTGGAAG ATGACGACCT AGATGAGTTT CTTGACGATG AAGAAAATGA GGAGGATGAC 
CAAGAAGAGG TTCAAGTCAG AAGTGAAAAA GAAGACAGTC CGAAGACATC AAAACGTGCA 
GAGAAATCGG CTAGAAACAT CAAACAGCAA GATTCGTTAT GTAGCGATCA TTCGGTAGAG 
CAGGCAAAAC CTTCCACAAG CAAGACCACA TCCAAAACCA ACGATAGAAA GACTGAAGAT 
GAAGTACTTT ACGCTCAACT TGCTCAAAAT GCCATCCGAA ACCAACAACC AAGAAAGAAC 
AATACTGGAG TCCAAATAGC GCCAGAGTTG TCAGATATTG TCATTTACAT GCAAGCGACA 
AAATTCAAAG GATTTCCCCC AGTCGATGGC ATTCAATCAC CAAGGATAAT GGAGGAAGGG 
CCAGCTTCTG CAAGTCTTTC ATTTTCATCA CGGGCTCGTA CGCCATCAAA TCTGCTGAAT 
ACACCAGCCC CTCCACGACG ACAGCGAAGT AGCACTCAGT TGAGTCAAGA ACTGGCTGCA 
GAATTTTTGG GATCAGTTAG GGCAAATGCT ACAGCTACAT GCTACCAGGT TACATCTCTT 
AATGAAAACG CTGCAAAGAA ACTGATGAAA AGACATCCAG CAAAATGCGT ATCTTATACT 
CGGGACCATC TGATTCGTAC GTATCCAAGT GCCAAGCACT ATGACTCGTC CAACTTTAAC 
CCCATCAACT GCTGGGCTCA TGGAATGCAA ATGGTTGCTT TAAATTTTCA AACTCCTGAT 
GTTATAATGG CAGTTAACCA AGCGATGTTC GAGCAATCTG GAAACTGTGG ATATCAACTG 
AAACCACGTT GTCTTTGGGA TGAATCTCAT CTTTTGTACA ACAAATTTTT GCCTCTCTCC 
AAAGACATTG CCGGCCATTC TGCTTTGTTA CTAAACTTAA CTATTATATC CGGACAGCAC 
GTGTATCCAA ACACCCATTA CGCCAGTTTA TATGTCGAAA TTGAAGTCAT CGGTATTCAC 
AACGACTGTG TTCGTGAGAA ATCAAAAGTG GTCCAGAGAA ATTCTGTGAA TCCAATCTGG 
AACCACACCA CTCAACTTCG AATAGCTTGT GTTGACTTAG CATTCCTTCG CATAGCAGTA 
TGTGATAGTG GGCAAAATGG AAGAGTCGTT GCTCACCGAG TTGTTCCTGT CAAATGCATT 
CGTCCGGGTT TCCGACATCT CCCACTCAGA ACTCCAACTA ATCTTCCGAT CGACAACGCT 
ATGATATTTT TAAGAACAAG ATTCGAGCAA GAAGAACACA TCTACCTCCA CGACGATGAT 
TCAAACACCT ATTGTAATCT AGAACATACG CTGGCATACC GCACAGACTT AACACCCAAT 
TTATCTCCAA CGCCAATTTT GAAGAAACAG ATCTTTGTTC TTCGAATTAC AGGCGCGTTT 
GCTGATGAAA CAGCAATTAC AGTGCACAGC GAGTCAGGAA GTACAGTGAA GACGGTGATG 
CAACAGGCGC TGCTTAACGC TGGAAAGAAT GCGGATCAGG TTGAAGAGTA TGTACTCATC 
GAAGAATCAC TGCCGGCACC TTCTGGGGAA GATCCAATTG AGCAGAGAGT ACTTCCTCTG 
AACGAGCCTA TCATGGACGC CGTGGCCTGC TGGAACGGTT CAATGCGTCG TTTTGTGTTA 
CGAAAAAAAG GCAGTGACCC ATCATCTAGA GCTTGGATCA CTTCAATTAT AAAATCCGGA 
ACAAGTGGAA GCTCCACGTC AGTCTCACCA AGTCCGTTGA CCAAAGATGG ACACGTAAAG 
AGTGCATCAA GCAATCAACT TCATGGACGA TCATTGGACA CTGACGCTTT TGGAGAGCAC 
CTTGAAGTGA CAGAAGGCAA GTGGTTGAAC CCTCGTGCAA GATCAATGGG CGACACATTT 
CTGGTATGTG TTCACAATGT TAGTGAAGAT CAGCCTTATG CAATCCTACG TGCTGGAATC 
CACAGCACTG CCGCCGACAT TATTCGTCAG GTGTTTGTTA AAGCTCGCCG TTCAAATGTA 
GACGATTCCG AGTTTGTTCT TGTTGAAGAA ACCTGCGATG ATCCAAAACT CAATCAAGGC 
CAAAGTATGC TACAAGCCCT TTCGTTGGCG CGGAAAAGAA GCAATGATCT GACTCCAAAG 
TATCCAAACA ACCGCACCAC AAGCCGAGTG TTAGGACAAA ACGAAAATGT TTGGAAAGCA 
CAATCGAGGT GGAAGTCAAT GGGCAGATTT GTACTTGAAA ACCGAAAGGA CACAGTGCAC 
GCAACCCTCG AAAAAGAGTT TCACGAAATG GCAAAAATCA TTCGTGAGGG TATTCCAAAA 
AAGGACGAAA CTTATTATAT GATATACTAC TCAGGACTGC CAGGAGAGGA TATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_001029446.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024617.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

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CloneID	OCa348502
Clone ID Related Accession (Same CDS sequence)	NM_001029446.3 , NM_001029446.2
Accession Version	NM_001029446.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_001029446.1. COMPLETENESS: incomplete on both ends.

ATGAACTGGG ACACTCTGAA AGGCGTGCTG AAAACAAGGC GTTTGACAAA ACGTACGATT 
CCAGCGTATA TTCATCCAAC ATCACGTAGT GACTCAACCT CTTCAACGCA ATCAGCCACA 
GCTGGATTCA TTCTCAATGA AGAACCAATC ACACTGTTCC GACTTGAACT CGAACGACTT 
CAATATATTC TTCATTTCCC TGAAGAAGTT GCATTCCAAC TATCTTCTAC TGAGTATCAG 
CTTTTCTACT CAATACAACC CATGGATTAT GTTCGTTACG TCAGTTGCGA TCTCACATCT 
GTTCCAGTGT CAGAAAATCC AAGTCCAGTG CGAAATCTTG TGAAAAGGCT ATCAGAGGTA 
TCATCCTGGA TAACACATGT TATTGTCAGT CAGCCAACAC ATGACGATCG AAAGGTTGCT 
CTTACTGCAA TTTTGAGAAT TGTGGAAACT TGCTGGAATA TTGGAAACTT TAATGCAGCT 
GTTGAGGTTC TTATGGGTTT AAAGAGTGAA AAACTCAGAC CATTCTGGCT TTCTTTGCGA 
CAAGAGGAAA AGTCTCAGTT TGATTCGCTT TGCGAAACCT TGCTTCCTGC AAACCAAGCT 
CTCCCATCTC AAGCTTACAT CAACGCGGTC CAACGAGCAC TGAGAATGCC ACAATCTCGT 
GTGATTCCAT TTTTTGGAAT ATTTCTTCGT GATCTCTACG CAATTGTGAA TGACTTGCCG 
AATATCGTTG TGATTGGACA AGAAGGTGAA ACCCAAAAGC TGGAATTCAT GAGCGATCCC 
AATGGCGAAG ATCATTTTTC GTCGAGAATT GGAGTGGGTG GGCTGTTGAA TGCTGATAAA 
ATCAACTTGG TGGCAATTGT TTTGGACAAC TTGGAATTAT TCCATCGTCA CAGCAGGACA 
ATGATCAAAT TGTTAGAAGA GCAAGCAGTC CCACCAATTC AAATTCCTCA GAACGAGCGG 
GAGCAAAAAG AAAAGGAAGC AAAAACATAT GAACCAGTAC AAGTGGTTCG CGGCTCTTCT 
CATGGAGTGG CTCTCATCCC TCTTGATACC CTAACATTCG ATCTTGATGT TATCCAACGA 
CTCCAGCACG GAACAACAGT GATCCATTAC GAACCTGATT CGGGAAGAAG CAATTTGTGT 
CTGCTGCGGT TAGACCCTAG TTGCGGTCAA ATAAACTGGC ATAAGATTTC ATATTCAGTT 
AACAAAGATC CCAAAGAAAA GGATGTGCTC GCCAAAGTAT CCGTTTCAAA TCTGCAGCCG 
CTAGACTCTG GCCGAGGGGC TCCGTCACCA ATGCCATCTG GAAGAACACC AGGAACTGGA 
GGAGTTGGAG TAGAGGAGGG AGAACTCAAA TTAAGTGTTG TGAAAGGAGT CGAACTAGTT 
GATAGTTATG ATATTGATAT TGAGGCAATT TACCGTCGTC ATTCTATGGA AGAGATGTCA 
GTTCCTGTTA GCTGTTGGAA AGTTAGTCAT GGTCAGTTGT TATCGGATAA TGAGTTCATC 
TACTTTTTGG CCCCTCAACA AATTGCTCAA TTTTGGACCA ACGGGTTGCA ATCTGTAGTT 
AAAAGTCTCC AAGGACAGCA AAGGTACCCT GATCGGCGAA TGTTATGGAT CAAAAATGTC 
TACTTATCAC TTTACGAGAT AACGGGCGAA TCAAACTGTG GACCACGGCC ATTTGAAGCA 
CTTCAGGCAT TTGGGCTGAG TCAAACAAAT ACTAACGCAA CACGACCCAA TGACTCGTCG 
CTGTCCAGTG AGCCAGGAGG AGCTAAAAGC AGACTGAAGA ACCTTAAAAA TGCTATGCAA 
AAGAAGCTTC GTGGTGCATC GCGCGAAGGA AGCAGATCTC AGTCACCTCA ACCTCATTCT 
CCTCTTGTCC GACCACCATC AATAAAATCG CAAATATCGT CACAATCTGG TCCGCCGGGT 
CCGAACTCTC CAGGCTATTT GCTGAAACCA CGTGGAGAAC CTGCCAATAG TGATGCAGGA 
GACATTGATA GCATTTATAC ACCTCGATCA AGAACTCCAA CAAGCAGCTC CTATGGAGGG 
CGATCCGTTG GAGGGAGATC ATGTAAATCG TGGAGATCAA GAGGAGGAGA GACACCAAAT 
TCGGGTTCCA TAAGCAGCTC GGGTCAGATG TCAATTCAAG TGTCTGGTCT GAGTGGACCA 
TCGGGAAAAG AGTTTCAGGA AAAACCATTA ACACTTGTAG AGTTCGCTGA ACTTTTTCGA 
TTGTTCAATA CAAGAATGAG AAAAGATTTG AGAGATGTGT TCAATGATGT CCTGTCTACG 
GCAACTACTC CTCAGCATTG TCCTAAAAGA GAACGTGATC GCCACAGTCC GAGAATGCAG 
TCAAGGCTGG CGAGTGTCTC CAACTCGTAC AATGCAGATT TTCTGTCTAA CGATTTCTTG 
ACTCGGAATA CGGCAGTAAC GTCCCATCAT ATCAGTGAAA AGCAAAACAA AATCTACAAT 
GCATTGGCAC TTGCGAGTGT TAATAGCATG GGCGGACTCA TGGACACTTC CCGGTCGTCG 
ATGCTGACGC CGCAAATGCT GCGCGCTTTT GTCAACACTC ATCAGATGGA GCAGATTGAC 
GAGCAAACGG CGATTAAGCT TATTCAGGAT CATGAACCTG ATGGTATCTG TCGACAAAAG 
AATCAAATGT CATTCGAAGG ATTCACTAGA TTCCTTTGCG ATCCTGTCAA CTTTGCATTC 
GTACCGGAAA CAATTGAACC AGATGAAGAG GACCTACGGT ACCCGTTGTC TCATTATTAC 
ATCAATTCAA GTCACAACAC CTATTTGACG GGACATCAAC TCAAGGGACC CAGTAGCTCG 
GAGATGTACA GACAGGTTCT CCTCACCGGG TGTCGGTGTG TCGAGCTTGA TTGTTGGGAT 
GGAGATGACG GATTGCCATT GATTTATCAT GGACACACAT TGGTTAGCAA GATTGGGTTT 
AGACAGGTGG TCGAAATTAT CAAAAAATCT GCATTCATAA CATCGGATCT TCCTGTGATA 
TTATCAATTG AGAATCATTG TTCCCTACAA CAACAGGCGA AAATGGCACA GATGTTCAAA 
ACAGTTCTTG GAGATTTACT AGTATCAAAC TTCCTTTTTG AAGCTGATTT CTCGGACTCT 
CCACGTCTTC CATGCCCTCT CCAAATGAAA AATAAGATCT TGATAAAAAA TAAGAAAATG 
ATAGTCGACC CACCAACTCC ATTGCCAATG ATTGAACGAG GAGCTGTGCA GCGTGGTGAA 
ACTCAATTAA ATCTTCACAG AAAACAGAGT AAAAACTCCT ACGAAAGCAG TACGGTAGAT 
GAAGTGGAAG ATGACGACCT AGATGAGTTT CTTGACGATG AAGAAAATGA GGAGGATGAC 
CAAGAAGAGG TTCAAGTCAG AAGTGAAAAA GAAGACAGTC CGAAGACATC AAAACGTGCA 
GAGAAATCGG CTAGAAACAT CAAACAGCAA GATTCGTTAT GTAGCGATCA TTCGGTAGAG 
CAGGCAAAAC CTTCCACAAG CAAGACCACA TCCAAAACCA ACGATAGAAA GACTGAAGAT 
GAAGTACTTT ACGCTCAACT TGCTCAAAAT GCCATCCGAA ACCAACAACC AAGAAAGAAC 
AATACTGGAG TCCAAATAGC GCCAGAGTTG TCAGATATTG TCATTTACAT GCAAGCGACA 
AAATTCAAAG GATTTCCCCC AGTCGATGGC ATTCAATCAC CAAGGATAAT GGAGGAAGGG 
CCAGCTTCTG CAAGTCTTTC ATTTTCATCA CGGGCTCGTA CGCCATCAAA TCTGCTGAAT 
ACACCAGCCC CTCCACGACG ACAGCGAAGT AGCACTCAGT TGAGTCAAGA ACTGGCTGCA 
GAATTTTTGG GATCAGTTAG GGCAAATGCT ACAGCTACAT GCTACCAGGT TACATCTCTT 
AATGAAAACG CTGCAAAGAA ACTGATGAAA AGACATCCAG CAAAATGCGT ATCTTATACT 
CGGGACCATC TGATTCGTAC GTATCCAAGT GCCAAGCACT ATGACTCGTC CAACTTTAAC 
CCCATCAACT GCTGGGCTCA TGGAATGCAA ATGGTTGCTT TAAATTTTCA AACTCCTGAT 
GTTATAATGG CAGTTAACCA AGCGATGTTC GAGCAATCTG GAAACTGTGG ATATCAACTG 
AAACCACGTT GTCTTTGGGA TGAATCTCAT CTTTTGTACA ACAAATTTTT GCCTCTCTCC 
AAAGACATTG CCGGCCATTC TGCTTTGTTA CTAAACTTAA CTATTATATC CGGACAGCAC 
GTGTATCCAA ACACCCATTA CGCCAGTTTA TATGTCGAAA TTGAAGTCAT CGGTATTCAC 
AACGACTGTG TTCGTGAGAA ATCAAAAGTG GTCCAGAGAA ATTCTGTGAA TCCAATCTGG 
AACCACACCA CTCAACTTCG AATAGCTTGT GTTGACTTAG CATTCCTTCG CATAGCAGTA 
TGTGATAGTG GGCAAAATGG AAGAGTCGTT GCTCACCGAG TTGTTCCTGT CAAATGCATT 
CGTCCGGGTT TCCGACATCT CCCACTCAGA ACTCCAACTA ATCTTCCGAT CGACAACGCT 
ATGATATTTT TAAGAACAAG ATTCGAGCAA GAAGAACACA TCTACCTCCA CGACGATGAT 
TCAAACACCT ATTGTAATCT AGAACATACG CTGGCATACC GCACAGACTT AACACCCAAT 
TTATCTCCAA CGCCAATTTT GAAGAAACAG ATCTTTGTTC TTCGAATTAC AGGCGCGTTT 
GCTGATGAAA CAGCAATTAC AGTGCACAGC GAGTCAGGAA GTACAGTGAA GACGGTGATG 
CAACAGGCGC TGCTTAACGC TGGAAAGAAT GCGGATCAGG TTGAAGAGTA TGTACTCATC 
GAAGAATCAC TGCCGGCACC TTCTGGGGAA GATCCAATTG AGCAGAGAGT ACTTCCTCTG 
AACGAGCCTA TCATGGACGC CGTGGCCTGC TGGAACGGTT CAATGCGTCG TTTTGTGTTA 
CGAAAAAAAG GCAGTGACCC ATCATCTAGA GCTTGGATCA CTTCAATTAT AAAATCCGGA 
ACAAGTGGAA GCTCCACGTC AGTCTCACCA AGTCCGTTGA CCAAAGATGG ACACGTAAAG 
AGTGCATCAA GCAATCAACT TCATGGACGA TCATTGGACA CTGACGCTTT TGGAGAGCAC 
CTTGAAGTGA CAGAAGGCAA GTGGTTGAAC CCTCGTGCAA GATCAATGGG CGACACATTT 
CTGGTATGTG TTCACAATGT TAGTGAAGAT CAGCCTTATG CAATCCTACG TGCTGGAATC 
CACAGCACTG CCGCCGACAT TATTCGTCAG GTGTTTGTTA AAGCTCGCCG TTCAAATGTA 
GACGATTCCG AGTTTGTTCT TGTTGAAGAA ACCTGCGATG ATCCAAAACT CAATCAAGGC 
CAAAGTATGC TACAAGCCCT TTCGTTGGCG CGGAAAAGAA GCAATGATCT GACTCCAAAG 
TATCCAAACA ACCGCACCAC AAGCCGAGTG TTAGGACAAA ACGAAAATGT TTGGAAAGCA 
CAATCGAGGT GGAAGTCAAT GGGCAGATTT GTACTTGAAA ACCGAAAGGA CACAGTGCAC 
GCAACCCTCG AAAAAGAGTT TCACGAAATG GCAAAAATCA TTCGTGAGGG TATTCCAAAA 
AAGGACGAAA CTTATTATAT GATATACTAC TCAGGACTGC CAGGAGAGGA TATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024617.1
CDS	1..5697
Translation	MNWDTLKGVLKTRRLTKRTIPAYIHPTSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGKWLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQSMLQALSLARKRSNDLTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKEFHEMAKIIREGIPKKDETYYMIYYSGLPGEDI

Target ORF information:

RefSeq Version	NM_001029446.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029446.2

ORF Insert Sequence:

ATGAACTGGG ACACTCTGAA AGGCGTGCTG AAAACAAGGC GTTTGACAAA ACGTACGATT 
CCAGCGTATA TTCATCCAAC ATCACGTAGT GACTCAACCT CTTCAACGCA ATCAGCCACA 
GCTGGATTCA TTCTCAATGA AGAACCAATC ACACTGTTCC GACTTGAACT CGAACGACTT 
CAATATATTC TTCATTTCCC TGAAGAAGTT GCATTCCAAC TATCTTCTAC TGAGTATCAG 
CTTTTCTACT CAATACAACC CATGGATTAT GTTCGTTACG TCAGTTGCGA TCTCACATCT 
GTTCCAGTGT CAGAAAATCC AAGTCCAGTG CGAAATCTTG TGAAAAGGCT ATCAGAGGTA 
TCATCCTGGA TAACACATGT TATTGTCAGT CAGCCAACAC ATGACGATCG AAAGGTTGCT 
CTTACTGCAA TTTTGAGAAT TGTGGAAACT TGCTGGAATA TTGGAAACTT TAATGCAGCT 
GTTGAGGTTC TTATGGGTTT AAAGAGTGAA AAACTCAGAC CATTCTGGCT TTCTTTGCGA 
CAAGAGGAAA AGTCTCAGTT TGATTCGCTT TGCGAAACCT TGCTTCCTGC AAACCAAGCT 
CTCCCATCTC AAGCTTACAT CAACGCGGTC CAACGAGCAC TGAGAATGCC ACAATCTCGT 
GTGATTCCAT TTTTTGGAAT ATTTCTTCGT GATCTCTACG CAATTGTGAA TGACTTGCCG 
AATATCGTTG TGATTGGACA AGAAGGTGAA ACCCAAAAGC TGGAATTCAT GAGCGATCCC 
AATGGCGAAG ATCATTTTTC GTCGAGAATT GGAGTGGGTG GGCTGTTGAA TGCTGATAAA 
ATCAACTTGG TGGCAATTGT TTTGGACAAC TTGGAATTAT TCCATCGTCA CAGCAGGACA 
ATGATCAAAT TGTTAGAAGA GCAAGCAGTC CCACCAATTC AAATTCCTCA GAACGAGCGG 
GAGCAAAAAG AAAAGGAAGC AAAAACATAT GAACCAGTAC AAGTGGTTCG CGGCTCTTCT 
CATGGAGTGG CTCTCATCCC TCTTGATACC CTAACATTCG ATCTTGATGT TATCCAACGA 
CTCCAGCACG GAACAACAGT GATCCATTAC GAACCTGATT CGGGAAGAAG CAATTTGTGT 
CTGCTGCGGT TAGACCCTAG TTGCGGTCAA ATAAACTGGC ATAAGATTTC ATATTCAGTT 
AACAAAGATC CCAAAGAAAA GGATGTGCTC GCCAAAGTAT CCGTTTCAAA TCTGCAGCCG 
CTAGACTCTG GCCGAGGGGC TCCGTCACCA ATGCCATCTG GAAGAACACC AGGAACTGGA 
GGAGTTGGAG TAGAGGAGGG AGAACTCAAA TTAAGTGTTG TGAAAGGAGT CGAACTAGTT 
GATAGTTATG ATATTGATAT TGAGGCAATT TACCGTCGTC ATTCTATGGA AGAGATGTCA 
GTTCCTGTTA GCTGTTGGAA AGTTAGTCAT GGTCAGTTGT TATCGGATAA TGAGTTCATC 
TACTTTTTGG CCCCTCAACA AATTGCTCAA TTTTGGACCA ACGGGTTGCA ATCTGTAGTT 
AAAAGTCTCC AAGGACAGCA AAGGTACCCT GATCGGCGAA TGTTATGGAT CAAAAATGTC 
TACTTATCAC TTTACGAGAT AACGGGCGAA TCAAACTGTG GACCACGGCC ATTTGAAGCA 
CTTCAGGCAT TTGGGCTGAG TCAAACAAAT ACTAACGCAA CACGACCCAA TGACTCGTCG 
CTGTCCAGTG AGCCAGGAGG AGCTAAAAGC AGACTGAAGA ACCTTAAAAA TGCTATGCAA 
AAGAAGCTTC GTGGTGCATC GCGCGAAGGA AGCAGATCTC AGTCACCTCA ACCTCATTCT 
CCTCTTGTCC GACCACCATC AATAAAATCG CAAATATCGT CACAATCTGG TCCGCCGGGT 
CCGAACTCTC CAGGCTATTT GCTGAAACCA CGTGGAGAAC CTGCCAATAG TGATGCAGGA 
GACATTGATA GCATTTATAC ACCTCGATCA AGAACTCCAA CAAGCAGCTC CTATGGAGGG 
CGATCCGTTG GAGGGAGATC ATGTAAATCG TGGAGATCAA GAGGAGGAGA GACACCAAAT 
TCGGGTTCCA TAAGCAGCTC GGGTCAGATG TCAATTCAAG TGTCTGGTCT GAGTGGACCA 
TCGGGAAAAG AGTTTCAGGA AAAACCATTA ACACTTGTAG AGTTCGCTGA ACTTTTTCGA 
TTGTTCAATA CAAGAATGAG AAAAGATTTG AGAGATGTGT TCAATGATGT CCTGTCTACG 
GCAACTACTC CTCAGCATTG TCCTAAAAGA GAACGTGATC GCCACAGTCC GAGAATGCAG 
TCAAGGCTGG CGAGTGTCTC CAACTCGTAC AATGCAGATT TTCTGTCTAA CGATTTCTTG 
ACTCGGAATA CGGCAGTAAC GTCCCATCAT ATCAGTGAAA AGCAAAACAA AATCTACAAT 
GCATTGGCAC TTGCGAGTGT TAATAGCATG GGCGGACTCA TGGACACTTC CCGGTCGTCG 
ATGCTGACGC CGCAAATGCT GCGCGCTTTT GTCAACACTC ATCAGATGGA GCAGATTGAC 
GAGCAAACGG CGATTAAGCT TATTCAGGAT CATGAACCTG ATGGTATCTG TCGACAAAAG 
AATCAAATGT CATTCGAAGG ATTCACTAGA TTCCTTTGCG ATCCTGTCAA CTTTGCATTC 
GTACCGGAAA CAATTGAACC AGATGAAGAG GACCTACGGT ACCCGTTGTC TCATTATTAC 
ATCAATTCAA GTCACAACAC CTATTTGACG GGACATCAAC TCAAGGGACC CAGTAGCTCG 
GAGATGTACA GACAGGTTCT CCTCACCGGG TGTCGGTGTG TCGAGCTTGA TTGTTGGGAT 
GGAGATGACG GATTGCCATT GATTTATCAT GGACACACAT TGGTTAGCAA GATTGGGTTT 
AGACAGGTGG TCGAAATTAT CAAAAAATCT GCATTCATAA CATCGGATCT TCCTGTGATA 
TTATCAATTG AGAATCATTG TTCCCTACAA CAACAGGCGA AAATGGCACA GATGTTCAAA 
ACAGTTCTTG GAGATTTACT AGTATCAAAC TTCCTTTTTG AAGCTGATTT CTCGGACTCT 
CCACGTCTTC CATGCCCTCT CCAAATGAAA AATAAGATCT TGATAAAAAA TAAGAAAATG 
ATAGTCGACC CACCAACTCC ATTGCCAATG ATTGAACGAG GAGCTGTGCA GCGTGGTGAA 
ACTCAATTAA ATCTTCACAG AAAACAGAGT AAAAACTCCT ACGAAAGCAG TACGGTAGAT 
GAAGTGGAAG ATGACGACCT AGATGAGTTT CTTGACGATG AAGAAAATGA GGAGGATGAC 
CAAGAAGAGG TTCAAGTCAG AAGTGAAAAA GAAGACAGTC CGAAGACATC AAAACGTGCA 
GAGAAATCGG CTAGAAACAT CAAACAGCAA GATTCGTTAT GTAGCGATCA TTCGGTAGAG 
CAGGCAAAAC CTTCCACAAG CAAGACCACA TCCAAAACCA ACGATAGAAA GACTGAAGAT 
GAAGTACTTT ACGCTCAACT TGCTCAAAAT GCCATCCGAA ACCAACAACC AAGAAAGAAC 
AATACTGGAG TCCAAATAGC GCCAGAGTTG TCAGATATTG TCATTTACAT GCAAGCGACA 
AAATTCAAAG GATTTCCCCC AGTCGATGGC ATTCAATCAC CAAGGATAAT GGAGGAAGGG 
CCAGCTTCTG CAAGTCTTTC ATTTTCATCA CGGGCTCGTA CGCCATCAAA TCTGCTGAAT 
ACACCAGCCC CTCCACGACG ACAGCGAAGT AGCACTCAGT TGAGTCAAGA ACTGGCTGCA 
GAATTTTTGG GATCAGTTAG GGCAAATGCT ACAGCTACAT GCTACCAGGT TACATCTCTT 
AATGAAAACG CTGCAAAGAA ACTGATGAAA AGACATCCAG CAAAATGCGT ATCTTATACT 
CGGGACCATC TGATTCGTAC GTATCCAAGT GCCAAGCACT ATGACTCGTC CAACTTTAAC 
CCCATCAACT GCTGGGCTCA TGGAATGCAA ATGGTTGCTT TAAATTTTCA AACTCCTGAT 
GTTATAATGG CAGTTAACCA AGCGATGTTC GAGCAATCTG GAAACTGTGG ATATCAACTG 
AAACCACGTT GTCTTTGGGA TGAATCTCAT CTTTTGTACA ACAAATTTTT GCCTCTCTCC 
AAAGACATTG CCGGCCATTC TGCTTTGTTA CTAAACTTAA CTATTATATC CGGACAGCAC 
GTGTATCCAA ACACCCATTA CGCCAGTTTA TATGTCGAAA TTGAAGTCAT CGGTATTCAC 
AACGACTGTG TTCGTGAGAA ATCAAAAGTG GTCCAGAGAA ATTCTGTGAA TCCAATCTGG 
AACCACACCA CTCAACTTCG AATAGCTTGT GTTGACTTAG CATTCCTTCG CATAGCAGTA 
TGTGATAGTG GGCAAAATGG AAGAGTCGTT GCTCACCGAG TTGTTCCTGT CAAATGCATT 
CGTCCGGGTT TCCGACATCT CCCACTCAGA ACTCCAACTA ATCTTCCGAT CGACAACGCT 
ATGATATTTT TAAGAACAAG ATTCGAGCAA GAAGAACACA TCTACCTCCA CGACGATGAT 
TCAAACACCT ATTGTAATCT AGAACATACG CTGGCATACC GCACAGACTT AACACCCAAT 
TTATCTCCAA CGCCAATTTT GAAGAAACAG ATCTTTGTTC TTCGAATTAC AGGCGCGTTT 
GCTGATGAAA CAGCAATTAC AGTGCACAGC GAGTCAGGAA GTACAGTGAA GACGGTGATG 
CAACAGGCGC TGCTTAACGC TGGAAAGAAT GCGGATCAGG TTGAAGAGTA TGTACTCATC 
GAAGAATCAC TGCCGGCACC TTCTGGGGAA GATCCAATTG AGCAGAGAGT ACTTCCTCTG 
AACGAGCCTA TCATGGACGC CGTGGCCTGC TGGAACGGTT CAATGCGTCG TTTTGTGTTA 
CGAAAAAAAG GCAGTGACCC ATCATCTAGA GCTTGGATCA CTTCAATTAT AAAATCCGGA 
ACAAGTGGAA GCTCCACGTC AGTCTCACCA AGTCCGTTGA CCAAAGATGG ACACGTAAAG 
AGTGCATCAA GCAATCAACT TCATGGACGA TCATTGGACA CTGACGCTTT TGGAGAGCAC 
CTTGAAGTGA CAGAAGGCAA GTGGTTGAAC CCTCGTGCAA GATCAATGGG CGACACATTT 
CTGGTATGTG TTCACAATGT TAGTGAAGAT CAGCCTTATG CAATCCTACG TGCTGGAATC 
CACAGCACTG CCGCCGACAT TATTCGTCAG GTGTTTGTTA AAGCTCGCCG TTCAAATGTA 
GACGATTCCG AGTTTGTTCT TGTTGAAGAA ACCTGCGATG ATCCAAAACT CAATCAAGGC 
CAAAGTATGC TACAAGCCCT TTCGTTGGCG CGGAAAAGAA GCAATGATCT GACTCCAAAG 
TATCCAAACA ACCGCACCAC AAGCCGAGTG TTAGGACAAA ACGAAAATGT TTGGAAAGCA 
CAATCGAGGT GGAAGTCAAT GGGCAGATTT GTACTTGAAA ACCGAAAGGA CACAGTGCAC 
GCAACCCTCG AAAAAGAGTT TCACGAAATG GCAAAAATCA TTCGTGAGGG TATTCCAAAA 
AAGGACGAAA CTTATTATAT GATATACTAC TCAGGACTGC CAGGAGAGGA TATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_077404.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_509805.3 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

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CloneID	OCa348503
Clone ID Related Accession (Same CDS sequence)	NM_077404.6 , NM_077404.5
Accession Version	NM_077404.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_077404.5. COMPLETENESS: incomplete on the 5' end.

ATGAACTGGG ACACTCTGAA AGGCGTGCTG AAAACAAGGC GTTTGACAAA ACGTACGATT 
CCAGCGTATA TTCATCCAAC ATCACGTAGT GACTCAACCT CTTCAACGCA ATCAGCCACA 
GCTGGATTCA TTCTCAATGA AGAACCAATC ACACTGTTCC GACTTGAACT CGAACGACTT 
CAATATATTC TTCATTTCCC TGAAGAAGTT GCATTCCAAC TATCTTCTAC TGAGTATCAG 
CTTTTCTACT CAATACAACC CATGGATTAT GTTCGTTACG TCAGTTGCGA TCTCACATCT 
GTTCCAGTGT CAGAAAATCC AAGTCCAGTG CGAAATCTTG TGAAAAGGCT ATCAGAGGTA 
TCATCCTGGA TAACACATGT TATTGTCAGT CAGCCAACAC ATGACGATCG AAAGGTTGCT 
CTTACTGCAA TTTTGAGAAT TGTGGAAACT TGCTGGAATA TTGGAAACTT TAATGCAGCT 
GTTGAGGTTC TTATGGGTTT AAAGAGTGAA AAACTCAGAC CATTCTGGCT TTCTTTGCGA 
CAAGAGGAAA AGTCTCAGTT TGATTCGCTT TGCGAAACCT TGCTTCCTGC AAACCAAGCT 
CTCCCATCTC AAGCTTACAT CAACGCGGTC CAACGAGCAC TGAGAATGCC ACAATCTCGT 
GTGATTCCAT TTTTTGGAAT ATTTCTTCGT GATCTCTACG CAATTGTGAA TGACTTGCCG 
AATATCGTTG TGATTGGACA AGAAGGTGAA ACCCAAAAGC TGGAATTCAT GAGCGATCCC 
AATGGCGAAG ATCATTTTTC GTCGAGAATT GGAGTGGGTG GGCTGTTGAA TGCTGATAAA 
ATCAACTTGG TGGCAATTGT TTTGGACAAC TTGGAATTAT TCCATCGTCA CAGCAGGACA 
ATGATCAAAT TGTTAGAAGA GCAAGCAGTC CCACCAATTC AAATTCCTCA GAACGAGCGG 
GAGCAAAAAG AAAAGGAAGC AAAAACATAT GAACCAGTAC AAGTGGTTCG CGGCTCTTCT 
CATGGAGTGG CTCTCATCCC TCTTGATACC CTAACATTCG ATCTTGATGT TATCCAACGA 
CTCCAGCACG GAACAACAGT GATCCATTAC GAACCTGATT CGGGAAGAAG CAATTTGTGT 
CTGCTGCGGT TAGACCCTAG TTGCGGTCAA ATAAACTGGC ATAAGATTTC ATATTCAGTT 
AACAAAGATC CCAAAGAAAA GGATGTGCTC GCCAAAGTAT CCGTTTCAAA TCTGCAGCCG 
CTAGACTCTG GCCGAGGGGC TCCGTCACCA ATGCCATCTG GAAGAACACC AGGAACTGGA 
GGAGTTGGAG TAGAGGAGGG AGAACTCAAA TTAAGTGTTG TGAAAGGAGT CGAACTAGTT 
GATAGTTATG ATATTGATAT TGAGGCAATT TACCGTCGTC ATTCTATGGA AGAGATGTCA 
GTTCCTGTTA GCTGTTGGAA AGTTAGTCAT GGTCAGTTGT TATCGGATAA TGAGTTCATC 
TACTTTTTGG CCCCTCAACA AATTGCTCAA TTTTGGACCA ACGGGTTGCA ATCTGTAGTT 
AAAAGTCTCC AAGGACAGCA AAGGTACCCT GATCGGCGAA TGTTATGGAT CAAAAATGTC 
TACTTATCAC TTTACGAGAT AACGGGCGAA TCAAACTGTG GACCACGGCC ATTTGAAGCA 
CTTCAGGCAT TTGGGCTGAG TCAAACAAAT ACTAACGCAA CACGACCCAA TGACTCGTCG 
CTGTCCAGTG AGCCAGGAGG AGCTAAAAGC AGACTGAAGA ACCTTAAAAA TGCTATGCAA 
AAGAAGCTTC GTGGTGCATC GCGCGAAGGA AGCAGATCTC AGTCACCTCA ACCTCATTCT 
CCTCTTGTCC GACCACCATC AATAAAATCG CAAATATCGT CACAATCTGG TCCGCCGGGT 
CCGAACTCTC CAGGCTATTT GCTGAAACCA CGTGGAGAAC CTGCCAATAG TGATGCAGGA 
GACATTGATA GCATTTATAC ACCTCGATCA AGAACTCCAA CAAGCAGCTC CTATGGAGGG 
CGATCCGTTG GAGGGAGATC ATGTAAATCG TGGAGATCAA GAGGAGGAGA GACACCAAAT 
TCGGGTTCCA TAAGCAGCTC GGGTCAGATG TCAATTCAAG TGTCTGGTCT GAGTGGACCA 
TCGGGAAAAG AGTTTCAGGA AAAACCATTA ACACTTGTAG AGTTCGCTGA ACTTTTTCGA 
TTGTTCAATA CAAGAATGAG AAAAGATTTG AGAGATGTGT TCAATGATGT CCTGTCTACG 
GCAACTACTC CTCAGCATTG TCCTAAAAGA GAACGTGATC GCCACAGTCC GAGAATGCAG 
TCAAGGCTGG CGAGTGTCTC CAACTCGTAC AATGCAGATT TTCTGTCTAA CGATTTCTTG 
ACTCGGAATA CGGCAGTAAC GTCCCATCAT ATCAGTGAAA AGCAAAACAA AATCTACAAT 
GCATTGGCAC TTGCGAGTGT TAATAGCATG GGCGGACTCA TGGACACTTC CCGGTCGTCG 
ATGCTGACGC CGCAAATGCT GCGCGCTTTT GTCAACACTC ATCAGATGGA GCAGATTGAC 
GAGCAAACGG CGATTAAGCT TATTCAGGAT CATGAACCTG ATGGTATCTG TCGACAAAAG 
AATCAAATGT CATTCGAAGG ATTCACTAGA TTCCTTTGCG ATCCTGTCAA CTTTGCATTC 
GTACCGGAAA CAATTGAACC AGATGAAGAG GACCTACGGT ACCCGTTGTC TCATTATTAC 
ATCAATTCAA GTCACAACAC CTATTTGACG GGACATCAAC TCAAGGGACC CAGTAGCTCG 
GAGATGTACA GACAGGTTCT CCTCACCGGG TGTCGGTGTG TCGAGCTTGA TTGTTGGGAT 
GGAGATGACG GATTGCCATT GATTTATCAT GGACACACAT TGGTTAGCAA GATTGGGTTT 
AGACAGGTGG TCGAAATTAT CAAAAAATCT GCATTCATAA CATCGGATCT TCCTGTGATA 
TTATCAATTG AGAATCATTG TTCCCTACAA CAACAGGCGA AAATGGCACA GATGTTCAAA 
ACAGTTCTTG GAGATTTACT AGTATCAAAC TTCCTTTTTG AAGCTGATTT CTCGGACTCT 
CCACGTCTTC CATGCCCTCT CCAAATGAAA AATAAGATCT TGATAAAAAA TAAGAAAATG 
ATAGTCGACC CACCAACTCC ATTGCCAATG ATTGAACGAG GAGCTGTGCA GCGTGGTGAA 
ACTCAATTAA ATCTTCACAG AAAACAGAGT AAAAACTCCT ACGAAAGCAG TACGGTAGAT 
GAAGTGGAAG ATGACGACCT AGATGAGTTT CTTGACGATG AAGAAAATGA GGAGGATGAC 
CAAGAAGAGG TTCAAGTCAG AAGTGAAAAA GAAGACAGTC CGAAGACATC AAAACGTGCA 
GAGAAATCGG CTAGAAACAT CAAACAGCAA GATTCGTTAT GTAGCGATCA TTCGGTAGAG 
CAGGCAAAAC CTTCCACAAG CAAGACCACA TCCAAAACCA ACGATAGAAA GACTGAAGAT 
GAAGTACTTT ACGCTCAACT TGCTCAAAAT GCCATCCGAA ACCAACAACC AAGAAAGAAC 
AATACTGGAG TCCAAATAGC GCCAGAGTTG TCAGATATTG TCATTTACAT GCAAGCGACA 
AAATTCAAAG GATTTCCCCC AGTCGATGGC ATTCAATCAC CAAGGATAAT GGAGGAAGGG 
CCAGCTTCTG CAAGTCTTTC ATTTTCATCA CGGGCTCGTA CGCCATCAAA TCTGCTGAAT 
ACACCAGCCC CTCCACGACG ACAGCGAAGT AGCACTCAGT TGAGTCAAGA ACTGGCTGCA 
GAATTTTTGG GATCAGTTAG GGCAAATGCT ACAGCTACAT GCTACCAGGT TACATCTCTT 
AATGAAAACG CTGCAAAGAA ACTGATGAAA AGACATCCAG CAAAATGCGT ATCTTATACT 
CGGGACCATC TGATTCGTAC GTATCCAAGT GCCAAGCACT ATGACTCGTC CAACTTTAAC 
CCCATCAACT GCTGGGCTCA TGGAATGCAA ATGGTTGCTT TAAATTTTCA AACTCCTGAT 
GTTATAATGG CAGTTAACCA AGCGATGTTC GAGCAATCTG GAAACTGTGG ATATCAACTG 
AAACCACGTT GTCTTTGGGA TGAATCTCAT CTTTTGTACA ACAAATTTTT GCCTCTCTCC 
AAAGACATTG CCGGCCATTC TGCTTTGTTA CTAAACTTAA CTATTATATC CGGACAGCAC 
GTGTATCCAA ACACCCATTA CGCCAGTTTA TATGTCGAAA TTGAAGTCAT CGGTATTCAC 
AACGACTGTG TTCGTGAGAA ATCAAAAGTG GTCCAGAGAA ATTCTGTGAA TCCAATCTGG 
AACCACACCA CTCAACTTCG AATAGCTTGT GTTGACTTAG CATTCCTTCG CATAGCAGTA 
TGTGATAGTG GGCAAAATGG AAGAGTCGTT GCTCACCGAG TTGTTCCTGT CAAATGCATT 
CGTCCGGGTT TCCGACATCT CCCACTCAGA ACTCCAACTA ATCTTCCGAT CGACAACGCT 
ATGATATTTT TAAGAACAAG ATTCGAGCAA GAAGAACACA TCTACCTCCA CGACGATGAT 
TCAAACACCT ATTGTAATCT AGAACATACG CTGGCATACC GCACAGACTT AACACCCAAT 
TTATCTCCAA CGCCAATTTT GAAGAAACAG ATCTTTGTTC TTCGAATTAC AGGCGCGTTT 
GCTGATGAAA CAGCAATTAC AGTGCACAGC GAGTCAGGAA GTACAGTGAA GACGGTGATG 
CAACAGGCGC TGCTTAACGC TGGAAAGAAT GCGGATCAGG TTGAAGAGTA TGTACTCATC 
GAAGAATCAC TGCCGGCACC TTCTGGGGAA GATCCAATTG AGCAGAGAGT ACTTCCTCTG 
AACGAGCCTA TCATGGACGC CGTGGCCTGC TGGAACGGTT CAATGCGTCG TTTTGTGTTA 
CGAAAAAAAG GCAGTGACCC ATCATCTAGA GCTTGGATCA CTTCAATTAT AAAATCCGGA 
ACAAGTGGAA GCTCCACGTC AGTCTCACCA AGTCCGTTGA CCAAAGATGG ACACGTAAAG 
AGTGCATCAA GCAATCAACT TCATGGACGA TCATTGGACA CTGACGCTTT TGGAGAGCAC 
CTTGAAGTGA CAGAAGGGTT GAACCCTCGT GCAAGATCAA TGGGCGACAC ATTTCTGGTA 
TGTGTTCACA ATGTTAGTGA AGATCAGCCT TATGCAATCC TACGTGCTGG AATCCACAGC 
ACTGCCGCCG ACATTATTCG TCAGGTGTTT GTTAAAGCTC GCCGTTCAAA TGTAGACGAT 
TCCGAGTTTG TTCTTGTTGA AGAAACCTGC GATGATCCAA AACTCAATCA AGGCCAAATG 
ACTCCAAAGT ATCCAAACAA CCGCACCACA AGCCGAGTGT TAGGACAAAA CGAAAATGTT 
TGGAAAGCAC AATCGAGGTG GAAGTCAATG GGCAGATTTG TACTTGAAAA CCGAAAGGAC 
ACAGTGCACG CAACCCTCGA AAAAGTGAGA TCATTTATAT CAAAACTGGA AGCTGCAAAA 
GCAAGCATGG ATCCCAGACA CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_509805.3
CDS	1..5607
Translation	MNWDTLKGVLKTRRLTKRTIPAYIHPTSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKVRSFISKLEAAKASMDPRHE

Target ORF information:

RefSeq Version	NM_077404.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_077404.6

ORF Insert Sequence:

ATGAACTGGG ACACTCTGAA AGGCGTGCTG AAAACAAGGC GTTTGACAAA ACGTACGATT 
CCAGCGTATA TTCATCCAAC ATCACGTAGT GACTCAACCT CTTCAACGCA ATCAGCCACA 
GCTGGATTCA TTCTCAATGA AGAACCAATC ACACTGTTCC GACTTGAACT CGAACGACTT 
CAATATATTC TTCATTTCCC TGAAGAAGTT GCATTCCAAC TATCTTCTAC TGAGTATCAG 
CTTTTCTACT CAATACAACC CATGGATTAT GTTCGTTACG TCAGTTGCGA TCTCACATCT 
GTTCCAGTGT CAGAAAATCC AAGTCCAGTG CGAAATCTTG TGAAAAGGCT ATCAGAGGTA 
TCATCCTGGA TAACACATGT TATTGTCAGT CAGCCAACAC ATGACGATCG AAAGGTTGCT 
CTTACTGCAA TTTTGAGAAT TGTGGAAACT TGCTGGAATA TTGGAAACTT TAATGCAGCT 
GTTGAGGTTC TTATGGGTTT AAAGAGTGAA AAACTCAGAC CATTCTGGCT TTCTTTGCGA 
CAAGAGGAAA AGTCTCAGTT TGATTCGCTT TGCGAAACCT TGCTTCCTGC AAACCAAGCT 
CTCCCATCTC AAGCTTACAT CAACGCGGTC CAACGAGCAC TGAGAATGCC ACAATCTCGT 
GTGATTCCAT TTTTTGGAAT ATTTCTTCGT GATCTCTACG CAATTGTGAA TGACTTGCCG 
AATATCGTTG TGATTGGACA AGAAGGTGAA ACCCAAAAGC TGGAATTCAT GAGCGATCCC 
AATGGCGAAG ATCATTTTTC GTCGAGAATT GGAGTGGGTG GGCTGTTGAA TGCTGATAAA 
ATCAACTTGG TGGCAATTGT TTTGGACAAC TTGGAATTAT TCCATCGTCA CAGCAGGACA 
ATGATCAAAT TGTTAGAAGA GCAAGCAGTC CCACCAATTC AAATTCCTCA GAACGAGCGG 
GAGCAAAAAG AAAAGGAAGC AAAAACATAT GAACCAGTAC AAGTGGTTCG CGGCTCTTCT 
CATGGAGTGG CTCTCATCCC TCTTGATACC CTAACATTCG ATCTTGATGT TATCCAACGA 
CTCCAGCACG GAACAACAGT GATCCATTAC GAACCTGATT CGGGAAGAAG CAATTTGTGT 
CTGCTGCGGT TAGACCCTAG TTGCGGTCAA ATAAACTGGC ATAAGATTTC ATATTCAGTT 
AACAAAGATC CCAAAGAAAA GGATGTGCTC GCCAAAGTAT CCGTTTCAAA TCTGCAGCCG 
CTAGACTCTG GCCGAGGGGC TCCGTCACCA ATGCCATCTG GAAGAACACC AGGAACTGGA 
GGAGTTGGAG TAGAGGAGGG AGAACTCAAA TTAAGTGTTG TGAAAGGAGT CGAACTAGTT 
GATAGTTATG ATATTGATAT TGAGGCAATT TACCGTCGTC ATTCTATGGA AGAGATGTCA 
GTTCCTGTTA GCTGTTGGAA AGTTAGTCAT GGTCAGTTGT TATCGGATAA TGAGTTCATC 
TACTTTTTGG CCCCTCAACA AATTGCTCAA TTTTGGACCA ACGGGTTGCA ATCTGTAGTT 
AAAAGTCTCC AAGGACAGCA AAGGTACCCT GATCGGCGAA TGTTATGGAT CAAAAATGTC 
TACTTATCAC TTTACGAGAT AACGGGCGAA TCAAACTGTG GACCACGGCC ATTTGAAGCA 
CTTCAGGCAT TTGGGCTGAG TCAAACAAAT ACTAACGCAA CACGACCCAA TGACTCGTCG 
CTGTCCAGTG AGCCAGGAGG AGCTAAAAGC AGACTGAAGA ACCTTAAAAA TGCTATGCAA 
AAGAAGCTTC GTGGTGCATC GCGCGAAGGA AGCAGATCTC AGTCACCTCA ACCTCATTCT 
CCTCTTGTCC GACCACCATC AATAAAATCG CAAATATCGT CACAATCTGG TCCGCCGGGT 
CCGAACTCTC CAGGCTATTT GCTGAAACCA CGTGGAGAAC CTGCCAATAG TGATGCAGGA 
GACATTGATA GCATTTATAC ACCTCGATCA AGAACTCCAA CAAGCAGCTC CTATGGAGGG 
CGATCCGTTG GAGGGAGATC ATGTAAATCG TGGAGATCAA GAGGAGGAGA GACACCAAAT 
TCGGGTTCCA TAAGCAGCTC GGGTCAGATG TCAATTCAAG TGTCTGGTCT GAGTGGACCA 
TCGGGAAAAG AGTTTCAGGA AAAACCATTA ACACTTGTAG AGTTCGCTGA ACTTTTTCGA 
TTGTTCAATA CAAGAATGAG AAAAGATTTG AGAGATGTGT TCAATGATGT CCTGTCTACG 
GCAACTACTC CTCAGCATTG TCCTAAAAGA GAACGTGATC GCCACAGTCC GAGAATGCAG 
TCAAGGCTGG CGAGTGTCTC CAACTCGTAC AATGCAGATT TTCTGTCTAA CGATTTCTTG 
ACTCGGAATA CGGCAGTAAC GTCCCATCAT ATCAGTGAAA AGCAAAACAA AATCTACAAT 
GCATTGGCAC TTGCGAGTGT TAATAGCATG GGCGGACTCA TGGACACTTC CCGGTCGTCG 
ATGCTGACGC CGCAAATGCT GCGCGCTTTT GTCAACACTC ATCAGATGGA GCAGATTGAC 
GAGCAAACGG CGATTAAGCT TATTCAGGAT CATGAACCTG ATGGTATCTG TCGACAAAAG 
AATCAAATGT CATTCGAAGG ATTCACTAGA TTCCTTTGCG ATCCTGTCAA CTTTGCATTC 
GTACCGGAAA CAATTGAACC AGATGAAGAG GACCTACGGT ACCCGTTGTC TCATTATTAC 
ATCAATTCAA GTCACAACAC CTATTTGACG GGACATCAAC TCAAGGGACC CAGTAGCTCG 
GAGATGTACA GACAGGTTCT CCTCACCGGG TGTCGGTGTG TCGAGCTTGA TTGTTGGGAT 
GGAGATGACG GATTGCCATT GATTTATCAT GGACACACAT TGGTTAGCAA GATTGGGTTT 
AGACAGGTGG TCGAAATTAT CAAAAAATCT GCATTCATAA CATCGGATCT TCCTGTGATA 
TTATCAATTG AGAATCATTG TTCCCTACAA CAACAGGCGA AAATGGCACA GATGTTCAAA 
ACAGTTCTTG GAGATTTACT AGTATCAAAC TTCCTTTTTG AAGCTGATTT CTCGGACTCT 
CCACGTCTTC CATGCCCTCT CCAAATGAAA AATAAGATCT TGATAAAAAA TAAGAAAATG 
ATAGTCGACC CACCAACTCC ATTGCCAATG ATTGAACGAG GAGCTGTGCA GCGTGGTGAA 
ACTCAATTAA ATCTTCACAG AAAACAGAGT AAAAACTCCT ACGAAAGCAG TACGGTAGAT 
GAAGTGGAAG ATGACGACCT AGATGAGTTT CTTGACGATG AAGAAAATGA GGAGGATGAC 
CAAGAAGAGG TTCAAGTCAG AAGTGAAAAA GAAGACAGTC CGAAGACATC AAAACGTGCA 
GAGAAATCGG CTAGAAACAT CAAACAGCAA GATTCGTTAT GTAGCGATCA TTCGGTAGAG 
CAGGCAAAAC CTTCCACAAG CAAGACCACA TCCAAAACCA ACGATAGAAA GACTGAAGAT 
GAAGTACTTT ACGCTCAACT TGCTCAAAAT GCCATCCGAA ACCAACAACC AAGAAAGAAC 
AATACTGGAG TCCAAATAGC GCCAGAGTTG TCAGATATTG TCATTTACAT GCAAGCGACA 
AAATTCAAAG GATTTCCCCC AGTCGATGGC ATTCAATCAC CAAGGATAAT GGAGGAAGGG 
CCAGCTTCTG CAAGTCTTTC ATTTTCATCA CGGGCTCGTA CGCCATCAAA TCTGCTGAAT 
ACACCAGCCC CTCCACGACG ACAGCGAAGT AGCACTCAGT TGAGTCAAGA ACTGGCTGCA 
GAATTTTTGG GATCAGTTAG GGCAAATGCT ACAGCTACAT GCTACCAGGT TACATCTCTT 
AATGAAAACG CTGCAAAGAA ACTGATGAAA AGACATCCAG CAAAATGCGT ATCTTATACT 
CGGGACCATC TGATTCGTAC GTATCCAAGT GCCAAGCACT ATGACTCGTC CAACTTTAAC 
CCCATCAACT GCTGGGCTCA TGGAATGCAA ATGGTTGCTT TAAATTTTCA AACTCCTGAT 
GTTATAATGG CAGTTAACCA AGCGATGTTC GAGCAATCTG GAAACTGTGG ATATCAACTG 
AAACCACGTT GTCTTTGGGA TGAATCTCAT CTTTTGTACA ACAAATTTTT GCCTCTCTCC 
AAAGACATTG CCGGCCATTC TGCTTTGTTA CTAAACTTAA CTATTATATC CGGACAGCAC 
GTGTATCCAA ACACCCATTA CGCCAGTTTA TATGTCGAAA TTGAAGTCAT CGGTATTCAC 
AACGACTGTG TTCGTGAGAA ATCAAAAGTG GTCCAGAGAA ATTCTGTGAA TCCAATCTGG 
AACCACACCA CTCAACTTCG AATAGCTTGT GTTGACTTAG CATTCCTTCG CATAGCAGTA 
TGTGATAGTG GGCAAAATGG AAGAGTCGTT GCTCACCGAG TTGTTCCTGT CAAATGCATT 
CGTCCGGGTT TCCGACATCT CCCACTCAGA ACTCCAACTA ATCTTCCGAT CGACAACGCT 
ATGATATTTT TAAGAACAAG ATTCGAGCAA GAAGAACACA TCTACCTCCA CGACGATGAT 
TCAAACACCT ATTGTAATCT AGAACATACG CTGGCATACC GCACAGACTT AACACCCAAT 
TTATCTCCAA CGCCAATTTT GAAGAAACAG ATCTTTGTTC TTCGAATTAC AGGCGCGTTT 
GCTGATGAAA CAGCAATTAC AGTGCACAGC GAGTCAGGAA GTACAGTGAA GACGGTGATG 
CAACAGGCGC TGCTTAACGC TGGAAAGAAT GCGGATCAGG TTGAAGAGTA TGTACTCATC 
GAAGAATCAC TGCCGGCACC TTCTGGGGAA GATCCAATTG AGCAGAGAGT ACTTCCTCTG 
AACGAGCCTA TCATGGACGC CGTGGCCTGC TGGAACGGTT CAATGCGTCG TTTTGTGTTA 
CGAAAAAAAG GCAGTGACCC ATCATCTAGA GCTTGGATCA CTTCAATTAT AAAATCCGGA 
ACAAGTGGAA GCTCCACGTC AGTCTCACCA AGTCCGTTGA CCAAAGATGG ACACGTAAAG 
AGTGCATCAA GCAATCAACT TCATGGACGA TCATTGGACA CTGACGCTTT TGGAGAGCAC 
CTTGAAGTGA CAGAAGGGTT GAACCCTCGT GCAAGATCAA TGGGCGACAC ATTTCTGGTA 
TGTGTTCACA ATGTTAGTGA AGATCAGCCT TATGCAATCC TACGTGCTGG AATCCACAGC 
ACTGCCGCCG ACATTATTCG TCAGGTGTTT GTTAAAGCTC GCCGTTCAAA TGTAGACGAT 
TCCGAGTTTG TTCTTGTTGA AGAAACCTGC GATGATCCAA AACTCAATCA AGGCCAAATG 
ACTCCAAAGT ATCCAAACAA CCGCACCACA AGCCGAGTGT TAGGACAAAA CGAAAATGTT 
TGGAAAGCAC AATCGAGGTG GAAGTCAATG GGCAGATTTG TACTTGAAAA CCGAAAGGAC 
ACAGTGCACG CAACCCTCGA AAAAGTGAGA TCATTTATAT CAAAACTGGA AGCTGCAAAA 
GCAAGCATGG ATCCCAGACA CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_077404.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_509805.3 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

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CloneID	OCa348503
Clone ID Related Accession (Same CDS sequence)	NM_077404.6 , NM_077404.5
Accession Version	NM_077404.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Nov 13, 2008 this sequence version replaced NM_077404.4. COMPLETENESS: incomplete on both ends.

ATGAACTGGG ACACTCTGAA AGGCGTGCTG AAAACAAGGC GTTTGACAAA ACGTACGATT 
CCAGCGTATA TTCATCCAAC ATCACGTAGT GACTCAACCT CTTCAACGCA ATCAGCCACA 
GCTGGATTCA TTCTCAATGA AGAACCAATC ACACTGTTCC GACTTGAACT CGAACGACTT 
CAATATATTC TTCATTTCCC TGAAGAAGTT GCATTCCAAC TATCTTCTAC TGAGTATCAG 
CTTTTCTACT CAATACAACC CATGGATTAT GTTCGTTACG TCAGTTGCGA TCTCACATCT 
GTTCCAGTGT CAGAAAATCC AAGTCCAGTG CGAAATCTTG TGAAAAGGCT ATCAGAGGTA 
TCATCCTGGA TAACACATGT TATTGTCAGT CAGCCAACAC ATGACGATCG AAAGGTTGCT 
CTTACTGCAA TTTTGAGAAT TGTGGAAACT TGCTGGAATA TTGGAAACTT TAATGCAGCT 
GTTGAGGTTC TTATGGGTTT AAAGAGTGAA AAACTCAGAC CATTCTGGCT TTCTTTGCGA 
CAAGAGGAAA AGTCTCAGTT TGATTCGCTT TGCGAAACCT TGCTTCCTGC AAACCAAGCT 
CTCCCATCTC AAGCTTACAT CAACGCGGTC CAACGAGCAC TGAGAATGCC ACAATCTCGT 
GTGATTCCAT TTTTTGGAAT ATTTCTTCGT GATCTCTACG CAATTGTGAA TGACTTGCCG 
AATATCGTTG TGATTGGACA AGAAGGTGAA ACCCAAAAGC TGGAATTCAT GAGCGATCCC 
AATGGCGAAG ATCATTTTTC GTCGAGAATT GGAGTGGGTG GGCTGTTGAA TGCTGATAAA 
ATCAACTTGG TGGCAATTGT TTTGGACAAC TTGGAATTAT TCCATCGTCA CAGCAGGACA 
ATGATCAAAT TGTTAGAAGA GCAAGCAGTC CCACCAATTC AAATTCCTCA GAACGAGCGG 
GAGCAAAAAG AAAAGGAAGC AAAAACATAT GAACCAGTAC AAGTGGTTCG CGGCTCTTCT 
CATGGAGTGG CTCTCATCCC TCTTGATACC CTAACATTCG ATCTTGATGT TATCCAACGA 
CTCCAGCACG GAACAACAGT GATCCATTAC GAACCTGATT CGGGAAGAAG CAATTTGTGT 
CTGCTGCGGT TAGACCCTAG TTGCGGTCAA ATAAACTGGC ATAAGATTTC ATATTCAGTT 
AACAAAGATC CCAAAGAAAA GGATGTGCTC GCCAAAGTAT CCGTTTCAAA TCTGCAGCCG 
CTAGACTCTG GCCGAGGGGC TCCGTCACCA ATGCCATCTG GAAGAACACC AGGAACTGGA 
GGAGTTGGAG TAGAGGAGGG AGAACTCAAA TTAAGTGTTG TGAAAGGAGT CGAACTAGTT 
GATAGTTATG ATATTGATAT TGAGGCAATT TACCGTCGTC ATTCTATGGA AGAGATGTCA 
GTTCCTGTTA GCTGTTGGAA AGTTAGTCAT GGTCAGTTGT TATCGGATAA TGAGTTCATC 
TACTTTTTGG CCCCTCAACA AATTGCTCAA TTTTGGACCA ACGGGTTGCA ATCTGTAGTT 
AAAAGTCTCC AAGGACAGCA AAGGTACCCT GATCGGCGAA TGTTATGGAT CAAAAATGTC 
TACTTATCAC TTTACGAGAT AACGGGCGAA TCAAACTGTG GACCACGGCC ATTTGAAGCA 
CTTCAGGCAT TTGGGCTGAG TCAAACAAAT ACTAACGCAA CACGACCCAA TGACTCGTCG 
CTGTCCAGTG AGCCAGGAGG AGCTAAAAGC AGACTGAAGA ACCTTAAAAA TGCTATGCAA 
AAGAAGCTTC GTGGTGCATC GCGCGAAGGA AGCAGATCTC AGTCACCTCA ACCTCATTCT 
CCTCTTGTCC GACCACCATC AATAAAATCG CAAATATCGT CACAATCTGG TCCGCCGGGT 
CCGAACTCTC CAGGCTATTT GCTGAAACCA CGTGGAGAAC CTGCCAATAG TGATGCAGGA 
GACATTGATA GCATTTATAC ACCTCGATCA AGAACTCCAA CAAGCAGCTC CTATGGAGGG 
CGATCCGTTG GAGGGAGATC ATGTAAATCG TGGAGATCAA GAGGAGGAGA GACACCAAAT 
TCGGGTTCCA TAAGCAGCTC GGGTCAGATG TCAATTCAAG TGTCTGGTCT GAGTGGACCA 
TCGGGAAAAG AGTTTCAGGA AAAACCATTA ACACTTGTAG AGTTCGCTGA ACTTTTTCGA 
TTGTTCAATA CAAGAATGAG AAAAGATTTG AGAGATGTGT TCAATGATGT CCTGTCTACG 
GCAACTACTC CTCAGCATTG TCCTAAAAGA GAACGTGATC GCCACAGTCC GAGAATGCAG 
TCAAGGCTGG CGAGTGTCTC CAACTCGTAC AATGCAGATT TTCTGTCTAA CGATTTCTTG 
ACTCGGAATA CGGCAGTAAC GTCCCATCAT ATCAGTGAAA AGCAAAACAA AATCTACAAT 
GCATTGGCAC TTGCGAGTGT TAATAGCATG GGCGGACTCA TGGACACTTC CCGGTCGTCG 
ATGCTGACGC CGCAAATGCT GCGCGCTTTT GTCAACACTC ATCAGATGGA GCAGATTGAC 
GAGCAAACGG CGATTAAGCT TATTCAGGAT CATGAACCTG ATGGTATCTG TCGACAAAAG 
AATCAAATGT CATTCGAAGG ATTCACTAGA TTCCTTTGCG ATCCTGTCAA CTTTGCATTC 
GTACCGGAAA CAATTGAACC AGATGAAGAG GACCTACGGT ACCCGTTGTC TCATTATTAC 
ATCAATTCAA GTCACAACAC CTATTTGACG GGACATCAAC TCAAGGGACC CAGTAGCTCG 
GAGATGTACA GACAGGTTCT CCTCACCGGG TGTCGGTGTG TCGAGCTTGA TTGTTGGGAT 
GGAGATGACG GATTGCCATT GATTTATCAT GGACACACAT TGGTTAGCAA GATTGGGTTT 
AGACAGGTGG TCGAAATTAT CAAAAAATCT GCATTCATAA CATCGGATCT TCCTGTGATA 
TTATCAATTG AGAATCATTG TTCCCTACAA CAACAGGCGA AAATGGCACA GATGTTCAAA 
ACAGTTCTTG GAGATTTACT AGTATCAAAC TTCCTTTTTG AAGCTGATTT CTCGGACTCT 
CCACGTCTTC CATGCCCTCT CCAAATGAAA AATAAGATCT TGATAAAAAA TAAGAAAATG 
ATAGTCGACC CACCAACTCC ATTGCCAATG ATTGAACGAG GAGCTGTGCA GCGTGGTGAA 
ACTCAATTAA ATCTTCACAG AAAACAGAGT AAAAACTCCT ACGAAAGCAG TACGGTAGAT 
GAAGTGGAAG ATGACGACCT AGATGAGTTT CTTGACGATG AAGAAAATGA GGAGGATGAC 
CAAGAAGAGG TTCAAGTCAG AAGTGAAAAA GAAGACAGTC CGAAGACATC AAAACGTGCA 
GAGAAATCGG CTAGAAACAT CAAACAGCAA GATTCGTTAT GTAGCGATCA TTCGGTAGAG 
CAGGCAAAAC CTTCCACAAG CAAGACCACA TCCAAAACCA ACGATAGAAA GACTGAAGAT 
GAAGTACTTT ACGCTCAACT TGCTCAAAAT GCCATCCGAA ACCAACAACC AAGAAAGAAC 
AATACTGGAG TCCAAATAGC GCCAGAGTTG TCAGATATTG TCATTTACAT GCAAGCGACA 
AAATTCAAAG GATTTCCCCC AGTCGATGGC ATTCAATCAC CAAGGATAAT GGAGGAAGGG 
CCAGCTTCTG CAAGTCTTTC ATTTTCATCA CGGGCTCGTA CGCCATCAAA TCTGCTGAAT 
ACACCAGCCC CTCCACGACG ACAGCGAAGT AGCACTCAGT TGAGTCAAGA ACTGGCTGCA 
GAATTTTTGG GATCAGTTAG GGCAAATGCT ACAGCTACAT GCTACCAGGT TACATCTCTT 
AATGAAAACG CTGCAAAGAA ACTGATGAAA AGACATCCAG CAAAATGCGT ATCTTATACT 
CGGGACCATC TGATTCGTAC GTATCCAAGT GCCAAGCACT ATGACTCGTC CAACTTTAAC 
CCCATCAACT GCTGGGCTCA TGGAATGCAA ATGGTTGCTT TAAATTTTCA AACTCCTGAT 
GTTATAATGG CAGTTAACCA AGCGATGTTC GAGCAATCTG GAAACTGTGG ATATCAACTG 
AAACCACGTT GTCTTTGGGA TGAATCTCAT CTTTTGTACA ACAAATTTTT GCCTCTCTCC 
AAAGACATTG CCGGCCATTC TGCTTTGTTA CTAAACTTAA CTATTATATC CGGACAGCAC 
GTGTATCCAA ACACCCATTA CGCCAGTTTA TATGTCGAAA TTGAAGTCAT CGGTATTCAC 
AACGACTGTG TTCGTGAGAA ATCAAAAGTG GTCCAGAGAA ATTCTGTGAA TCCAATCTGG 
AACCACACCA CTCAACTTCG AATAGCTTGT GTTGACTTAG CATTCCTTCG CATAGCAGTA 
TGTGATAGTG GGCAAAATGG AAGAGTCGTT GCTCACCGAG TTGTTCCTGT CAAATGCATT 
CGTCCGGGTT TCCGACATCT CCCACTCAGA ACTCCAACTA ATCTTCCGAT CGACAACGCT 
ATGATATTTT TAAGAACAAG ATTCGAGCAA GAAGAACACA TCTACCTCCA CGACGATGAT 
TCAAACACCT ATTGTAATCT AGAACATACG CTGGCATACC GCACAGACTT AACACCCAAT 
TTATCTCCAA CGCCAATTTT GAAGAAACAG ATCTTTGTTC TTCGAATTAC AGGCGCGTTT 
GCTGATGAAA CAGCAATTAC AGTGCACAGC GAGTCAGGAA GTACAGTGAA GACGGTGATG 
CAACAGGCGC TGCTTAACGC TGGAAAGAAT GCGGATCAGG TTGAAGAGTA TGTACTCATC 
GAAGAATCAC TGCCGGCACC TTCTGGGGAA GATCCAATTG AGCAGAGAGT ACTTCCTCTG 
AACGAGCCTA TCATGGACGC CGTGGCCTGC TGGAACGGTT CAATGCGTCG TTTTGTGTTA 
CGAAAAAAAG GCAGTGACCC ATCATCTAGA GCTTGGATCA CTTCAATTAT AAAATCCGGA 
ACAAGTGGAA GCTCCACGTC AGTCTCACCA AGTCCGTTGA CCAAAGATGG ACACGTAAAG 
AGTGCATCAA GCAATCAACT TCATGGACGA TCATTGGACA CTGACGCTTT TGGAGAGCAC 
CTTGAAGTGA CAGAAGGGTT GAACCCTCGT GCAAGATCAA TGGGCGACAC ATTTCTGGTA 
TGTGTTCACA ATGTTAGTGA AGATCAGCCT TATGCAATCC TACGTGCTGG AATCCACAGC 
ACTGCCGCCG ACATTATTCG TCAGGTGTTT GTTAAAGCTC GCCGTTCAAA TGTAGACGAT 
TCCGAGTTTG TTCTTGTTGA AGAAACCTGC GATGATCCAA AACTCAATCA AGGCCAAATG 
ACTCCAAAGT ATCCAAACAA CCGCACCACA AGCCGAGTGT TAGGACAAAA CGAAAATGTT 
TGGAAAGCAC AATCGAGGTG GAAGTCAATG GGCAGATTTG TACTTGAAAA CCGAAAGGAC 
ACAGTGCACG CAACCCTCGA AAAAGTGAGA TCATTTATAT CAAAACTGGA AGCTGCAAAA 
GCAAGCATGG ATCCCAGACA CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_509805.3
CDS	1..5607
Translation	MNWDTLKGVLKTRRLTKRTIPAYIHPTSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKVRSFISKLEAAKASMDPRHE

Target ORF information:

RefSeq Version	NM_077404.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_077404.5

ORF Insert Sequence:

ATGAACTGGG ACACTCTGAA AGGCGTGCTG AAAACAAGGC GTTTGACAAA ACGTACGATT 
CCAGCGTATA TTCATCCAAC ATCACGTAGT GACTCAACCT CTTCAACGCA ATCAGCCACA 
GCTGGATTCA TTCTCAATGA AGAACCAATC ACACTGTTCC GACTTGAACT CGAACGACTT 
CAATATATTC TTCATTTCCC TGAAGAAGTT GCATTCCAAC TATCTTCTAC TGAGTATCAG 
CTTTTCTACT CAATACAACC CATGGATTAT GTTCGTTACG TCAGTTGCGA TCTCACATCT 
GTTCCAGTGT CAGAAAATCC AAGTCCAGTG CGAAATCTTG TGAAAAGGCT ATCAGAGGTA 
TCATCCTGGA TAACACATGT TATTGTCAGT CAGCCAACAC ATGACGATCG AAAGGTTGCT 
CTTACTGCAA TTTTGAGAAT TGTGGAAACT TGCTGGAATA TTGGAAACTT TAATGCAGCT 
GTTGAGGTTC TTATGGGTTT AAAGAGTGAA AAACTCAGAC CATTCTGGCT TTCTTTGCGA 
CAAGAGGAAA AGTCTCAGTT TGATTCGCTT TGCGAAACCT TGCTTCCTGC AAACCAAGCT 
CTCCCATCTC AAGCTTACAT CAACGCGGTC CAACGAGCAC TGAGAATGCC ACAATCTCGT 
GTGATTCCAT TTTTTGGAAT ATTTCTTCGT GATCTCTACG CAATTGTGAA TGACTTGCCG 
AATATCGTTG TGATTGGACA AGAAGGTGAA ACCCAAAAGC TGGAATTCAT GAGCGATCCC 
AATGGCGAAG ATCATTTTTC GTCGAGAATT GGAGTGGGTG GGCTGTTGAA TGCTGATAAA 
ATCAACTTGG TGGCAATTGT TTTGGACAAC TTGGAATTAT TCCATCGTCA CAGCAGGACA 
ATGATCAAAT TGTTAGAAGA GCAAGCAGTC CCACCAATTC AAATTCCTCA GAACGAGCGG 
GAGCAAAAAG AAAAGGAAGC AAAAACATAT GAACCAGTAC AAGTGGTTCG CGGCTCTTCT 
CATGGAGTGG CTCTCATCCC TCTTGATACC CTAACATTCG ATCTTGATGT TATCCAACGA 
CTCCAGCACG GAACAACAGT GATCCATTAC GAACCTGATT CGGGAAGAAG CAATTTGTGT 
CTGCTGCGGT TAGACCCTAG TTGCGGTCAA ATAAACTGGC ATAAGATTTC ATATTCAGTT 
AACAAAGATC CCAAAGAAAA GGATGTGCTC GCCAAAGTAT CCGTTTCAAA TCTGCAGCCG 
CTAGACTCTG GCCGAGGGGC TCCGTCACCA ATGCCATCTG GAAGAACACC AGGAACTGGA 
GGAGTTGGAG TAGAGGAGGG AGAACTCAAA TTAAGTGTTG TGAAAGGAGT CGAACTAGTT 
GATAGTTATG ATATTGATAT TGAGGCAATT TACCGTCGTC ATTCTATGGA AGAGATGTCA 
GTTCCTGTTA GCTGTTGGAA AGTTAGTCAT GGTCAGTTGT TATCGGATAA TGAGTTCATC 
TACTTTTTGG CCCCTCAACA AATTGCTCAA TTTTGGACCA ACGGGTTGCA ATCTGTAGTT 
AAAAGTCTCC AAGGACAGCA AAGGTACCCT GATCGGCGAA TGTTATGGAT CAAAAATGTC 
TACTTATCAC TTTACGAGAT AACGGGCGAA TCAAACTGTG GACCACGGCC ATTTGAAGCA 
CTTCAGGCAT TTGGGCTGAG TCAAACAAAT ACTAACGCAA CACGACCCAA TGACTCGTCG 
CTGTCCAGTG AGCCAGGAGG AGCTAAAAGC AGACTGAAGA ACCTTAAAAA TGCTATGCAA 
AAGAAGCTTC GTGGTGCATC GCGCGAAGGA AGCAGATCTC AGTCACCTCA ACCTCATTCT 
CCTCTTGTCC GACCACCATC AATAAAATCG CAAATATCGT CACAATCTGG TCCGCCGGGT 
CCGAACTCTC CAGGCTATTT GCTGAAACCA CGTGGAGAAC CTGCCAATAG TGATGCAGGA 
GACATTGATA GCATTTATAC ACCTCGATCA AGAACTCCAA CAAGCAGCTC CTATGGAGGG 
CGATCCGTTG GAGGGAGATC ATGTAAATCG TGGAGATCAA GAGGAGGAGA GACACCAAAT 
TCGGGTTCCA TAAGCAGCTC GGGTCAGATG TCAATTCAAG TGTCTGGTCT GAGTGGACCA 
TCGGGAAAAG AGTTTCAGGA AAAACCATTA ACACTTGTAG AGTTCGCTGA ACTTTTTCGA 
TTGTTCAATA CAAGAATGAG AAAAGATTTG AGAGATGTGT TCAATGATGT CCTGTCTACG 
GCAACTACTC CTCAGCATTG TCCTAAAAGA GAACGTGATC GCCACAGTCC GAGAATGCAG 
TCAAGGCTGG CGAGTGTCTC CAACTCGTAC AATGCAGATT TTCTGTCTAA CGATTTCTTG 
ACTCGGAATA CGGCAGTAAC GTCCCATCAT ATCAGTGAAA AGCAAAACAA AATCTACAAT 
GCATTGGCAC TTGCGAGTGT TAATAGCATG GGCGGACTCA TGGACACTTC CCGGTCGTCG 
ATGCTGACGC CGCAAATGCT GCGCGCTTTT GTCAACACTC ATCAGATGGA GCAGATTGAC 
GAGCAAACGG CGATTAAGCT TATTCAGGAT CATGAACCTG ATGGTATCTG TCGACAAAAG 
AATCAAATGT CATTCGAAGG ATTCACTAGA TTCCTTTGCG ATCCTGTCAA CTTTGCATTC 
GTACCGGAAA CAATTGAACC AGATGAAGAG GACCTACGGT ACCCGTTGTC TCATTATTAC 
ATCAATTCAA GTCACAACAC CTATTTGACG GGACATCAAC TCAAGGGACC CAGTAGCTCG 
GAGATGTACA GACAGGTTCT CCTCACCGGG TGTCGGTGTG TCGAGCTTGA TTGTTGGGAT 
GGAGATGACG GATTGCCATT GATTTATCAT GGACACACAT TGGTTAGCAA GATTGGGTTT 
AGACAGGTGG TCGAAATTAT CAAAAAATCT GCATTCATAA CATCGGATCT TCCTGTGATA 
TTATCAATTG AGAATCATTG TTCCCTACAA CAACAGGCGA AAATGGCACA GATGTTCAAA 
ACAGTTCTTG GAGATTTACT AGTATCAAAC TTCCTTTTTG AAGCTGATTT CTCGGACTCT 
CCACGTCTTC CATGCCCTCT CCAAATGAAA AATAAGATCT TGATAAAAAA TAAGAAAATG 
ATAGTCGACC CACCAACTCC ATTGCCAATG ATTGAACGAG GAGCTGTGCA GCGTGGTGAA 
ACTCAATTAA ATCTTCACAG AAAACAGAGT AAAAACTCCT ACGAAAGCAG TACGGTAGAT 
GAAGTGGAAG ATGACGACCT AGATGAGTTT CTTGACGATG AAGAAAATGA GGAGGATGAC 
CAAGAAGAGG TTCAAGTCAG AAGTGAAAAA GAAGACAGTC CGAAGACATC AAAACGTGCA 
GAGAAATCGG CTAGAAACAT CAAACAGCAA GATTCGTTAT GTAGCGATCA TTCGGTAGAG 
CAGGCAAAAC CTTCCACAAG CAAGACCACA TCCAAAACCA ACGATAGAAA GACTGAAGAT 
GAAGTACTTT ACGCTCAACT TGCTCAAAAT GCCATCCGAA ACCAACAACC AAGAAAGAAC 
AATACTGGAG TCCAAATAGC GCCAGAGTTG TCAGATATTG TCATTTACAT GCAAGCGACA 
AAATTCAAAG GATTTCCCCC AGTCGATGGC ATTCAATCAC CAAGGATAAT GGAGGAAGGG 
CCAGCTTCTG CAAGTCTTTC ATTTTCATCA CGGGCTCGTA CGCCATCAAA TCTGCTGAAT 
ACACCAGCCC CTCCACGACG ACAGCGAAGT AGCACTCAGT TGAGTCAAGA ACTGGCTGCA 
GAATTTTTGG GATCAGTTAG GGCAAATGCT ACAGCTACAT GCTACCAGGT TACATCTCTT 
AATGAAAACG CTGCAAAGAA ACTGATGAAA AGACATCCAG CAAAATGCGT ATCTTATACT 
CGGGACCATC TGATTCGTAC GTATCCAAGT GCCAAGCACT ATGACTCGTC CAACTTTAAC 
CCCATCAACT GCTGGGCTCA TGGAATGCAA ATGGTTGCTT TAAATTTTCA AACTCCTGAT 
GTTATAATGG CAGTTAACCA AGCGATGTTC GAGCAATCTG GAAACTGTGG ATATCAACTG 
AAACCACGTT GTCTTTGGGA TGAATCTCAT CTTTTGTACA ACAAATTTTT GCCTCTCTCC 
AAAGACATTG CCGGCCATTC TGCTTTGTTA CTAAACTTAA CTATTATATC CGGACAGCAC 
GTGTATCCAA ACACCCATTA CGCCAGTTTA TATGTCGAAA TTGAAGTCAT CGGTATTCAC 
AACGACTGTG TTCGTGAGAA ATCAAAAGTG GTCCAGAGAA ATTCTGTGAA TCCAATCTGG 
AACCACACCA CTCAACTTCG AATAGCTTGT GTTGACTTAG CATTCCTTCG CATAGCAGTA 
TGTGATAGTG GGCAAAATGG AAGAGTCGTT GCTCACCGAG TTGTTCCTGT CAAATGCATT 
CGTCCGGGTT TCCGACATCT CCCACTCAGA ACTCCAACTA ATCTTCCGAT CGACAACGCT 
ATGATATTTT TAAGAACAAG ATTCGAGCAA GAAGAACACA TCTACCTCCA CGACGATGAT 
TCAAACACCT ATTGTAATCT AGAACATACG CTGGCATACC GCACAGACTT AACACCCAAT 
TTATCTCCAA CGCCAATTTT GAAGAAACAG ATCTTTGTTC TTCGAATTAC AGGCGCGTTT 
GCTGATGAAA CAGCAATTAC AGTGCACAGC GAGTCAGGAA GTACAGTGAA GACGGTGATG 
CAACAGGCGC TGCTTAACGC TGGAAAGAAT GCGGATCAGG TTGAAGAGTA TGTACTCATC 
GAAGAATCAC TGCCGGCACC TTCTGGGGAA GATCCAATTG AGCAGAGAGT ACTTCCTCTG 
AACGAGCCTA TCATGGACGC CGTGGCCTGC TGGAACGGTT CAATGCGTCG TTTTGTGTTA 
CGAAAAAAAG GCAGTGACCC ATCATCTAGA GCTTGGATCA CTTCAATTAT AAAATCCGGA 
ACAAGTGGAA GCTCCACGTC AGTCTCACCA AGTCCGTTGA CCAAAGATGG ACACGTAAAG 
AGTGCATCAA GCAATCAACT TCATGGACGA TCATTGGACA CTGACGCTTT TGGAGAGCAC 
CTTGAAGTGA CAGAAGGGTT GAACCCTCGT GCAAGATCAA TGGGCGACAC ATTTCTGGTA 
TGTGTTCACA ATGTTAGTGA AGATCAGCCT TATGCAATCC TACGTGCTGG AATCCACAGC 
ACTGCCGCCG ACATTATTCG TCAGGTGTTT GTTAAAGCTC GCCGTTCAAA TGTAGACGAT 
TCCGAGTTTG TTCTTGTTGA AGAAACCTGC GATGATCCAA AACTCAATCA AGGCCAAATG 
ACTCCAAAGT ATCCAAACAA CCGCACCACA AGCCGAGTGT TAGGACAAAA CGAAAATGTT 
TGGAAAGCAC AATCGAGGTG GAAGTCAATG GGCAGATTTG TACTTGAAAA CCGAAAGGAC 
ACAGTGCACG CAACCCTCGA AAAAGTGAGA TCATTTATAT CAAAACTGGA AGCTGCAAAA 
GCAAGCATGG ATCCCAGACA CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_001129669.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001123141.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

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CloneID	OCa348940
Clone ID Related Accession (Same CDS sequence)	NM_001129669.2
Accession Version	NM_001129669.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_001129669.1. COMPLETENESS: incomplete on both ends.

ATGACATTAT GGGAGATTAT GCAAAATTCT GCTAGCAATT CAATCCAGCG GAGTCTCAAC 
CAGTCAATGC ATCGTTCGCA CACCGAGGCG ACTGTCTCAA CATCACGTAG TGACTCAACC 
TCTTCAACGC AATCAGCCAC AGCTGGATTC ATTCTCAATG AAGAACCAAT CACACTGTTC 
CGACTTGAAC TCGAACGACT TCAATATATT CTTCATTTCC CTGAAGAAGT TGCATTCCAA 
CTATCTTCTA CTGAGTATCA GCTTTTCTAC TCAATACAAC CCATGGATTA TGTTCGTTAC 
GTCAGTTGCG ATCTCACATC TGTTCCAGTG TCAGAAAATC CAAGTCCAGT GCGAAATCTT 
GTGAAAAGGC TATCAGAGGT ATCATCCTGG ATAACACATG TTATTGTCAG TCAGCCAACA 
CATGACGATC GAAAGGTTGC TCTTACTGCA ATTTTGAGAA TTGTGGAAAC TTGCTGGAAT 
ATTGGAAACT TTAATGCAGC TGTTGAGGTT CTTATGGGTT TAAAGAGTGA AAAACTCAGA 
CCATTCTGGC TTTCTTTGCG ACAAGAGGAA AAGTCTCAGT TTGATTCGCT TTGCGAAACC 
TTGCTTCCTG CAAACCAAGC TCTCCCATCT CAAGCTTACA TCAACGCGGT CCAACGAGCA 
CTGAGAATGC CACAATCTCG TGTGATTCCA TTTTTTGGAA TATTTCTTCG TGATCTCTAC 
GCAATTGTGA ATGACTTGCC GAATATCGTT GTGATTGGAC AAGAAGGTGA AACCCAAAAG 
CTGGAATTCA TGAGCGATCC CAATGGCGAA GATCATTTTT CGTCGAGAAT TGGAGTGGGT 
GGGCTGTTGA ATGCTGATAA AATCAACTTG GTGGCAATTG TTTTGGACAA CTTGGAATTA 
TTCCATCGTC ACAGCAGGAC AATGATCAAA TTGTTAGAAG AGCAAGCAGT CCCACCAATT 
CAAATTCCTC AGAACGAGCG GGAGCAAAAA GAAAAGGAAG CAAAAACATA TGAACCAGTA 
CAAGTGGTTC GCGGCTCTTC TCATGGAGTG GCTCTCATCC CTCTTGATAC CCTAACATTC 
GATCTTGATG TTATCCAACG ACTCCAGCAC GGAACAACAG TGATCCATTA CGAACCTGAT 
TCGGGAAGAA GCAATTTGTG TCTGCTGCGG TTAGACCCTA GTTGCGGTCA AATAAACTGG 
CATAAGATTT CATATTCAGT TAACAAAGAT CCCAAAGAAA AGGATGTGCT CGCCAAAGTA 
TCCGTTTCAA ATCTGCAGCC GCTAGACTCT GGCCGAGGGG CTCCGTCACC AATGCCATCT 
GGAAGAACAC CAGGAACTGG AGGAGTTGGA GTAGAGGAGG GAGAACTCAA ATTAAGTGTT 
GTGAAAGGAG TCGAACTAGT TGATAGTTAT GATATTGATA TTGAGGCAAT TTACCGTCGT 
CATTCTATGG AAGAGATGTC AGTTCCTGTT AGCTGTTGGA AAGTTAGTCA TGGTCAGTTG 
TTATCGGATA ATGAGTTCAT CTACTTTTTG GCCCCTCAAC AAATTGCTCA ATTTTGGACC 
AACGGGTTGC AATCTGTAGT TAAAAGTCTC CAAGGACAGC AAAGGTACCC TGATCGGCGA 
ATGTTATGGA TCAAAAATGT CTACTTATCA CTTTACGAGA TAACGGGCGA ATCAAACTGT 
GGACCACGGC CATTTGAAGC ACTTCAGGCA TTTGGGCTGA GTCAAACAAA TACTAACGCA 
ACACGACCCA ATGACTCGTC GCTGTCCAGT GAGCCAGGAG GAGCTAAAAG CAGACTGAAG 
AACCTTAAAA ATGCTATGCA AAAGAAGCTT CGTGGTGCAT CGCGCGAAGG AAGCAGATCT 
CAGTCACCTC AACCTCATTC TCCTCTTGTC CGACCACCAT CAATAAAATC GCAAATATCG 
TCACAATCTG GTCCGCCGGG TCCGAACTCT CCAGGCTATT TGCTGAAACC ACGTGGAGAA 
CCTGCCAATA GTGATGCAGG AGACATTGAT AGCATTTATA CACCTCGATC AAGAACTCCA 
ACAAGCAGCT CCTATGGAGG GCGATCCGTT GGAGGGAGAT CATGTAAATC GTGGAGATCA 
AGAGGAGGAG AGACACCAAA TTCGGGTTCC ATAAGCAGCT CGGGTCAGAT GTCAATTCAA 
GTGTCTGGTC TGAGTGGACC ATCGGGAAAA GAGTTTCAGG AAAAACCATT AACACTTGTA 
GAGTTCGCTG AACTTTTTCG ATTGTTCAAT ACAAGAATGA GAAAAGATTT GAGAGATGTG 
TTCAATGATG TCCTGTCTAC GGCAACTACT CCTCAGCATT GTCCTAAAAG AGAACGTGAT 
CGCCACAGTC CGAGAATGCA GTCAAGGCTG GCGAGTGTCT CCAACTCGTA CAATGCAGAT 
TTTCTGTCTA ACGATTTCTT GACTCGGAAT ACGGCAGTAA CGTCCCATCA TATCAGTGAA 
AAGCAAAACA AAATCTACAA TGCATTGGCA CTTGCGAGTG TTAATAGCAT GGGCGGACTC 
ATGGACACTT CCCGGTCGTC GATGCTGACG CCGCAAATGC TGCGCGCTTT TGTCAACACT 
CATCAGATGG AGCAGATTGA CGAGCAAACG GCGATTAAGC TTATTCAGGA TCATGAACCT 
GATGGTATCT GTCGACAAAA GAATCAAATG TCATTCGAAG GATTCACTAG ATTCCTTTGC 
GATCCTGTCA ACTTTGCATT CGTACCGGAA ACAATTGAAC CAGATGAAGA GGACCTACGG 
TACCCGTTGT CTCATTATTA CATCAATTCA AGTCACAACA CCTATTTGAC GGGACATCAA 
CTCAAGGGAC CCAGTAGCTC GGAGATGTAC AGACAGGTTC TCCTCACCGG GTGTCGGTGT 
GTCGAGCTTG ATTGTTGGGA TGGAGATGAC GGATTGCCAT TGATTTATCA TGGACACACA 
TTGGTTAGCA AGATTGGGTT TAGACAGGTG GTCGAAATTA TCAAAAAATC TGCATTCATA 
ACATCGGATC TTCCTGTGAT ATTATCAATT GAGAATCATT GTTCCCTACA ACAACAGGCG 
AAAATGGCAC AGATGTTCAA AACAGTTCTT GGAGATTTAC TAGTATCAAA CTTCCTTTTT 
GAAGCTGATT TCTCGGACTC TCCACGTCTT CCATGCCCTC TCCAAATGAA AAATAAGATC 
TTGATAAAAA ATAAGAAAAT GATAGTCGAC CCACCAACTC CATTGCCAAT GATTGAACGA 
GGAGCTGTGC AGCGTGGTGA AACTCAATTA AATCTTCACA GAAAACAGAG TAAAAACTCC 
TACGAAAGCA GTACGGTAGA TGAAGTGGAA GATGACGACC TAGATGAGTT TCTTGACGAT 
GAAGAAAATG AGGAGGATGA CCAAGAAGAG GTTCAAGTCA GAAGTGAAAA AGAAGACAGT 
CCGAAGACAT CAAAACGTGC AGAGAAATCG GCTAGAAACA TCAAACAGCA AGATTCGTTA 
TGTAGCGATC ATTCGGTAGA GCAGGCAAAA CCTTCCACAA GCAAGACCAC ATCCAAAACC 
AACGATAGAA AGACTGAAGA TGAAGTACTT TACGCTCAAC TTGCTCAAAA TGCCATCCGA 
AACCAACAAC CAAGAAAGAA CAATACTGGA GTCCAAATAG CGCCAGAGTT GTCAGATATT 
GTCATTTACA TGCAAGCGAC AAAATTCAAA GGATTTCCCC CAGTCGATGG CATTCAATCA 
CCAAGGATAA TGGAGGAAGG GCCAGCTTCT GCAAGTCTTT CATTTTCATC ACGGGCTCGT 
ACGCCATCAA ATCTGCTGAA TACACCAGCC CCTCCACGAC GACAGCGAAG TAGCACTCAG 
TTGAGTCAAG AACTGGCTGC AGAATTTTTG GGATCAGTTA GGGCAAATGC TACAGCTACA 
TGCTACCAGG TTACATCTCT TAATGAAAAC GCTGCAAAGA AACTGATGAA AAGACATCCA 
GCAAAATGCG TATCTTATAC TCGGGACCAT CTGATTCGTA CGTATCCAAG TGCCAAGCAC 
TATGACTCGT CCAACTTTAA CCCCATCAAC TGCTGGGCTC ATGGAATGCA AATGGTTGCT 
TTAAATTTTC AAACTCCTGA TGTTATAATG GCAGTTAACC AAGCGATGTT CGAGCAATCT 
GGAAACTGTG GATATCAACT GAAACCACGT TGTCTTTGGG ATGAATCTCA TCTTTTGTAC 
AACAAATTTT TGCCTCTCTC CAAAGACATT GCCGGCCATT CTGCTTTGTT ACTAAACTTA 
ACTATTATAT CCGGACAGCA CGTGTATCCA AACACCCATT ACGCCAGTTT ATATGTCGAA 
ATTGAAGTCA TCGGTATTCA CAACGACTGT GTTCGTGAGA AATCAAAAGT GGTCCAGAGA 
AATTCTGTGA ATCCAATCTG GAACCACACC ACTCAACTTC GAATAGCTTG TGTTGACTTA 
GCATTCCTTC GCATAGCAGT ATGTGATAGT GGGCAAAATG GAAGAGTCGT TGCTCACCGA 
GTTGTTCCTG TCAAATGCAT TCGTCCGGGT TTCCGACATC TCCCACTCAG AACTCCAACT 
AATCTTCCGA TCGACAACGC TATGATATTT TTAAGAACAA GATTCGAGCA AGAAGAACAC 
ATCTACCTCC ACGACGATGA TTCAAACACC TATTGTAATC TAGAACATAC GCTGGCATAC 
CGCACAGACT TAACACCCAA TTTATCTCCA ACGCCAATTT TGAAGAAACA GATCTTTGTT 
CTTCGAATTA CAGGCGCGTT TGCTGATGAA ACAGCAATTA CAGTGCACAG CGAGTCAGGA 
AGTACAGTGA AGACGGTGAT GCAACAGGCG CTGCTTAACG CTGGAAAGAA TGCGGATCAG 
GTTGAAGAGT ATGTACTCAT CGAAGAATCA CTGCCGGCAC CTTCTGGGGA AGATCCAATT 
GAGCAGAGAG TACTTCCTCT GAACGAGCCT ATCATGGACG CCGTGGCCTG CTGGAACGGT 
TCAATGCGTC GTTTTGTGTT ACGAAAAAAA GGCAGTGACC CATCATCTAG AGCTTGGATC 
ACTTCAATTA TAAAATCCGG AACAAGTGGA AGCTCCACGT CAGTCTCACC AAGTCCGTTG 
ACCAAAGATG GACACGTAAA GAGTGCATCA AGCAATCAAC TTCATGGACG ATCATTGGAC 
ACTGACGCTT TTGGAGAGCA CCTTGAAGTG ACAGAAGGGT TGAACCCTCG TGCAAGATCA 
ATGGGCGACA CATTTCTGGT ATGTGTTCAC AATGTTAGTG AAGATCAGCC TTATGCAATC 
CTACGTGCTG GAATCCACAG CACTGCCGCC GACATTATTC GTCAGGTGTT TGTTAAAGCT 
CGCCGTTCAA ATGTAGACGA TTCCGAGTTT GTTCTTGTTG AAGAAACCTG CGATGATCCA 
AAACTCAATC AAGGCCAAAT GACTCCAAAG TATCCAAACA ACCGCACCAC AAGCCGAGTG 
TTAGGACAAA ACGAAAATGT TTGGAAAGCA CAATCGAGGT GGAAGTCAAT GGGCAGATTT 
GTACTTGAAA ACCGAAAGGA CACAGTGCAC GCAACCCTCG AAAAAGTGAG ATCATTTATA 
TCAAAACTGG AAGCTGCAAA AGCAAGCATG GATCCCAGAC ACGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123141.1
CDS	1..5628
Translation	MTLWEIMQNSASNSIQRSLNQSMHRSHTEATVSTSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKVRSFISKLEAAKASMDPRHE

Target ORF information:

RefSeq Version	NM_001129669.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001129669.2

ORF Insert Sequence:

ATGACATTAT GGGAGATTAT GCAAAATTCT GCTAGCAATT CAATCCAGCG GAGTCTCAAC 
CAGTCAATGC ATCGTTCGCA CACCGAGGCG ACTGTCTCAA CATCACGTAG TGACTCAACC 
TCTTCAACGC AATCAGCCAC AGCTGGATTC ATTCTCAATG AAGAACCAAT CACACTGTTC 
CGACTTGAAC TCGAACGACT TCAATATATT CTTCATTTCC CTGAAGAAGT TGCATTCCAA 
CTATCTTCTA CTGAGTATCA GCTTTTCTAC TCAATACAAC CCATGGATTA TGTTCGTTAC 
GTCAGTTGCG ATCTCACATC TGTTCCAGTG TCAGAAAATC CAAGTCCAGT GCGAAATCTT 
GTGAAAAGGC TATCAGAGGT ATCATCCTGG ATAACACATG TTATTGTCAG TCAGCCAACA 
CATGACGATC GAAAGGTTGC TCTTACTGCA ATTTTGAGAA TTGTGGAAAC TTGCTGGAAT 
ATTGGAAACT TTAATGCAGC TGTTGAGGTT CTTATGGGTT TAAAGAGTGA AAAACTCAGA 
CCATTCTGGC TTTCTTTGCG ACAAGAGGAA AAGTCTCAGT TTGATTCGCT TTGCGAAACC 
TTGCTTCCTG CAAACCAAGC TCTCCCATCT CAAGCTTACA TCAACGCGGT CCAACGAGCA 
CTGAGAATGC CACAATCTCG TGTGATTCCA TTTTTTGGAA TATTTCTTCG TGATCTCTAC 
GCAATTGTGA ATGACTTGCC GAATATCGTT GTGATTGGAC AAGAAGGTGA AACCCAAAAG 
CTGGAATTCA TGAGCGATCC CAATGGCGAA GATCATTTTT CGTCGAGAAT TGGAGTGGGT 
GGGCTGTTGA ATGCTGATAA AATCAACTTG GTGGCAATTG TTTTGGACAA CTTGGAATTA 
TTCCATCGTC ACAGCAGGAC AATGATCAAA TTGTTAGAAG AGCAAGCAGT CCCACCAATT 
CAAATTCCTC AGAACGAGCG GGAGCAAAAA GAAAAGGAAG CAAAAACATA TGAACCAGTA 
CAAGTGGTTC GCGGCTCTTC TCATGGAGTG GCTCTCATCC CTCTTGATAC CCTAACATTC 
GATCTTGATG TTATCCAACG ACTCCAGCAC GGAACAACAG TGATCCATTA CGAACCTGAT 
TCGGGAAGAA GCAATTTGTG TCTGCTGCGG TTAGACCCTA GTTGCGGTCA AATAAACTGG 
CATAAGATTT CATATTCAGT TAACAAAGAT CCCAAAGAAA AGGATGTGCT CGCCAAAGTA 
TCCGTTTCAA ATCTGCAGCC GCTAGACTCT GGCCGAGGGG CTCCGTCACC AATGCCATCT 
GGAAGAACAC CAGGAACTGG AGGAGTTGGA GTAGAGGAGG GAGAACTCAA ATTAAGTGTT 
GTGAAAGGAG TCGAACTAGT TGATAGTTAT GATATTGATA TTGAGGCAAT TTACCGTCGT 
CATTCTATGG AAGAGATGTC AGTTCCTGTT AGCTGTTGGA AAGTTAGTCA TGGTCAGTTG 
TTATCGGATA ATGAGTTCAT CTACTTTTTG GCCCCTCAAC AAATTGCTCA ATTTTGGACC 
AACGGGTTGC AATCTGTAGT TAAAAGTCTC CAAGGACAGC AAAGGTACCC TGATCGGCGA 
ATGTTATGGA TCAAAAATGT CTACTTATCA CTTTACGAGA TAACGGGCGA ATCAAACTGT 
GGACCACGGC CATTTGAAGC ACTTCAGGCA TTTGGGCTGA GTCAAACAAA TACTAACGCA 
ACACGACCCA ATGACTCGTC GCTGTCCAGT GAGCCAGGAG GAGCTAAAAG CAGACTGAAG 
AACCTTAAAA ATGCTATGCA AAAGAAGCTT CGTGGTGCAT CGCGCGAAGG AAGCAGATCT 
CAGTCACCTC AACCTCATTC TCCTCTTGTC CGACCACCAT CAATAAAATC GCAAATATCG 
TCACAATCTG GTCCGCCGGG TCCGAACTCT CCAGGCTATT TGCTGAAACC ACGTGGAGAA 
CCTGCCAATA GTGATGCAGG AGACATTGAT AGCATTTATA CACCTCGATC AAGAACTCCA 
ACAAGCAGCT CCTATGGAGG GCGATCCGTT GGAGGGAGAT CATGTAAATC GTGGAGATCA 
AGAGGAGGAG AGACACCAAA TTCGGGTTCC ATAAGCAGCT CGGGTCAGAT GTCAATTCAA 
GTGTCTGGTC TGAGTGGACC ATCGGGAAAA GAGTTTCAGG AAAAACCATT AACACTTGTA 
GAGTTCGCTG AACTTTTTCG ATTGTTCAAT ACAAGAATGA GAAAAGATTT GAGAGATGTG 
TTCAATGATG TCCTGTCTAC GGCAACTACT CCTCAGCATT GTCCTAAAAG AGAACGTGAT 
CGCCACAGTC CGAGAATGCA GTCAAGGCTG GCGAGTGTCT CCAACTCGTA CAATGCAGAT 
TTTCTGTCTA ACGATTTCTT GACTCGGAAT ACGGCAGTAA CGTCCCATCA TATCAGTGAA 
AAGCAAAACA AAATCTACAA TGCATTGGCA CTTGCGAGTG TTAATAGCAT GGGCGGACTC 
ATGGACACTT CCCGGTCGTC GATGCTGACG CCGCAAATGC TGCGCGCTTT TGTCAACACT 
CATCAGATGG AGCAGATTGA CGAGCAAACG GCGATTAAGC TTATTCAGGA TCATGAACCT 
GATGGTATCT GTCGACAAAA GAATCAAATG TCATTCGAAG GATTCACTAG ATTCCTTTGC 
GATCCTGTCA ACTTTGCATT CGTACCGGAA ACAATTGAAC CAGATGAAGA GGACCTACGG 
TACCCGTTGT CTCATTATTA CATCAATTCA AGTCACAACA CCTATTTGAC GGGACATCAA 
CTCAAGGGAC CCAGTAGCTC GGAGATGTAC AGACAGGTTC TCCTCACCGG GTGTCGGTGT 
GTCGAGCTTG ATTGTTGGGA TGGAGATGAC GGATTGCCAT TGATTTATCA TGGACACACA 
TTGGTTAGCA AGATTGGGTT TAGACAGGTG GTCGAAATTA TCAAAAAATC TGCATTCATA 
ACATCGGATC TTCCTGTGAT ATTATCAATT GAGAATCATT GTTCCCTACA ACAACAGGCG 
AAAATGGCAC AGATGTTCAA AACAGTTCTT GGAGATTTAC TAGTATCAAA CTTCCTTTTT 
GAAGCTGATT TCTCGGACTC TCCACGTCTT CCATGCCCTC TCCAAATGAA AAATAAGATC 
TTGATAAAAA ATAAGAAAAT GATAGTCGAC CCACCAACTC CATTGCCAAT GATTGAACGA 
GGAGCTGTGC AGCGTGGTGA AACTCAATTA AATCTTCACA GAAAACAGAG TAAAAACTCC 
TACGAAAGCA GTACGGTAGA TGAAGTGGAA GATGACGACC TAGATGAGTT TCTTGACGAT 
GAAGAAAATG AGGAGGATGA CCAAGAAGAG GTTCAAGTCA GAAGTGAAAA AGAAGACAGT 
CCGAAGACAT CAAAACGTGC AGAGAAATCG GCTAGAAACA TCAAACAGCA AGATTCGTTA 
TGTAGCGATC ATTCGGTAGA GCAGGCAAAA CCTTCCACAA GCAAGACCAC ATCCAAAACC 
AACGATAGAA AGACTGAAGA TGAAGTACTT TACGCTCAAC TTGCTCAAAA TGCCATCCGA 
AACCAACAAC CAAGAAAGAA CAATACTGGA GTCCAAATAG CGCCAGAGTT GTCAGATATT 
GTCATTTACA TGCAAGCGAC AAAATTCAAA GGATTTCCCC CAGTCGATGG CATTCAATCA 
CCAAGGATAA TGGAGGAAGG GCCAGCTTCT GCAAGTCTTT CATTTTCATC ACGGGCTCGT 
ACGCCATCAA ATCTGCTGAA TACACCAGCC CCTCCACGAC GACAGCGAAG TAGCACTCAG 
TTGAGTCAAG AACTGGCTGC AGAATTTTTG GGATCAGTTA GGGCAAATGC TACAGCTACA 
TGCTACCAGG TTACATCTCT TAATGAAAAC GCTGCAAAGA AACTGATGAA AAGACATCCA 
GCAAAATGCG TATCTTATAC TCGGGACCAT CTGATTCGTA CGTATCCAAG TGCCAAGCAC 
TATGACTCGT CCAACTTTAA CCCCATCAAC TGCTGGGCTC ATGGAATGCA AATGGTTGCT 
TTAAATTTTC AAACTCCTGA TGTTATAATG GCAGTTAACC AAGCGATGTT CGAGCAATCT 
GGAAACTGTG GATATCAACT GAAACCACGT TGTCTTTGGG ATGAATCTCA TCTTTTGTAC 
AACAAATTTT TGCCTCTCTC CAAAGACATT GCCGGCCATT CTGCTTTGTT ACTAAACTTA 
ACTATTATAT CCGGACAGCA CGTGTATCCA AACACCCATT ACGCCAGTTT ATATGTCGAA 
ATTGAAGTCA TCGGTATTCA CAACGACTGT GTTCGTGAGA AATCAAAAGT GGTCCAGAGA 
AATTCTGTGA ATCCAATCTG GAACCACACC ACTCAACTTC GAATAGCTTG TGTTGACTTA 
GCATTCCTTC GCATAGCAGT ATGTGATAGT GGGCAAAATG GAAGAGTCGT TGCTCACCGA 
GTTGTTCCTG TCAAATGCAT TCGTCCGGGT TTCCGACATC TCCCACTCAG AACTCCAACT 
AATCTTCCGA TCGACAACGC TATGATATTT TTAAGAACAA GATTCGAGCA AGAAGAACAC 
ATCTACCTCC ACGACGATGA TTCAAACACC TATTGTAATC TAGAACATAC GCTGGCATAC 
CGCACAGACT TAACACCCAA TTTATCTCCA ACGCCAATTT TGAAGAAACA GATCTTTGTT 
CTTCGAATTA CAGGCGCGTT TGCTGATGAA ACAGCAATTA CAGTGCACAG CGAGTCAGGA 
AGTACAGTGA AGACGGTGAT GCAACAGGCG CTGCTTAACG CTGGAAAGAA TGCGGATCAG 
GTTGAAGAGT ATGTACTCAT CGAAGAATCA CTGCCGGCAC CTTCTGGGGA AGATCCAATT 
GAGCAGAGAG TACTTCCTCT GAACGAGCCT ATCATGGACG CCGTGGCCTG CTGGAACGGT 
TCAATGCGTC GTTTTGTGTT ACGAAAAAAA GGCAGTGACC CATCATCTAG AGCTTGGATC 
ACTTCAATTA TAAAATCCGG AACAAGTGGA AGCTCCACGT CAGTCTCACC AAGTCCGTTG 
ACCAAAGATG GACACGTAAA GAGTGCATCA AGCAATCAAC TTCATGGACG ATCATTGGAC 
ACTGACGCTT TTGGAGAGCA CCTTGAAGTG ACAGAAGGGT TGAACCCTCG TGCAAGATCA 
ATGGGCGACA CATTTCTGGT ATGTGTTCAC AATGTTAGTG AAGATCAGCC TTATGCAATC 
CTACGTGCTG GAATCCACAG CACTGCCGCC GACATTATTC GTCAGGTGTT TGTTAAAGCT 
CGCCGTTCAA ATGTAGACGA TTCCGAGTTT GTTCTTGTTG AAGAAACCTG CGATGATCCA 
AAACTCAATC AAGGCCAAAT GACTCCAAAG TATCCAAACA ACCGCACCAC AAGCCGAGTG 
TTAGGACAAA ACGAAAATGT TTGGAAAGCA CAATCGAGGT GGAAGTCAAT GGGCAGATTT 
GTACTTGAAA ACCGAAAGGA CACAGTGCAC GCAACCCTCG AAAAAGTGAG ATCATTTATA 
TCAAAACTGG AAGCTGCAAA AGCAAGCATG GATCCCAGAC ACGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_001029448.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024619.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

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CloneID	OCa348985
Clone ID Related Accession (Same CDS sequence)	NM_001029448.4 , NM_001029448.3
Accession Version	NM_001029448.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_001029448.3. COMPLETENESS: incomplete on the 5' end.

ATGAACTGGG ACACTCTGAA AGGCGTGCTG AAAACAAGGC GTTTGACAAA ACGTACGATT 
CCAGCGTATA TTCATCCAAC ATCACGTAGT GACTCAACCT CTTCAACGCA ATCAGCCACA 
GCTGGATTCA TTCTCAATGA AGAACCAATC ACACTGTTCC GACTTGAACT CGAACGACTT 
CAATATATTC TTCATTTCCC TGAAGAAGTT GCATTCCAAC TATCTTCTAC TGAGTATCAG 
CTTTTCTACT CAATACAACC CATGGATTAT GTTCGTTACG TCAGTTGCGA TCTCACATCT 
GTTCCAGTGT CAGAAAATCC AAGTCCAGTG CGAAATCTTG TGAAAAGGCT ATCAGAGGTA 
TCATCCTGGA TAACACATGT TATTGTCAGT CAGCCAACAC ATGACGATCG AAAGGTTGCT 
CTTACTGCAA TTTTGAGAAT TGTGGAAACT TGCTGGAATA TTGGAAACTT TAATGCAGCT 
GTTGAGGTTC TTATGGGTTT AAAGAGTGAA AAACTCAGAC CATTCTGGCT TTCTTTGCGA 
CAAGAGGAAA AGTCTCAGTT TGATTCGCTT TGCGAAACCT TGCTTCCTGC AAACCAAGCT 
CTCCCATCTC AAGCTTACAT CAACGCGGTC CAACGAGCAC TGAGAATGCC ACAATCTCGT 
GTGATTCCAT TTTTTGGAAT ATTTCTTCGT GATCTCTACG CAATTGTGAA TGACTTGCCG 
AATATCGTTG TGATTGGACA AGAAGGTGAA ACCCAAAAGC TGGAATTCAT GAGCGATCCC 
AATGGCGAAG ATCATTTTTC GTCGAGAATT GGAGTGGGTG GGCTGTTGAA TGCTGATAAA 
ATCAACTTGG TGGCAATTGT TTTGGACAAC TTGGAATTAT TCCATCGTCA CAGCAGGACA 
ATGATCAAAT TGTTAGAAGA GCAAGCAGTC CCACCAATTC AAATTCCTCA GAACGAGCGG 
GAGCAAAAAG AAAAGGAAGC AAAAACATAT GAACCAGTAC AAGTGGTTCG CGGCTCTTCT 
CATGGAGTGG CTCTCATCCC TCTTGATACC CTAACATTCG ATCTTGATGT TATCCAACGA 
CTCCAGCACG GAACAACAGT GATCCATTAC GAACCTGATT CGGGAAGAAG CAATTTGTGT 
CTGCTGCGGT TAGACCCTAG TTGCGGTCAA ATAAACTGGC ATAAGATTTC ATATTCAGTT 
AACAAAGATC CCAAAGAAAA GGATGTGCTC GCCAAAGTAT CCGTTTCAAA TCTGCAGCCG 
CTAGACTCTG GCCGAGGGGC TCCGTCACCA ATGCCATCTG GAAGAACACC AGGAACTGGA 
GGAGTTGGAG TAGAGGAGGG AGAACTCAAA TTAAGTGTTG TGAAAGGAGT CGAACTAGTT 
GATAGTTATG ATATTGATAT TGAGGCAATT TACCGTCGTC ATTCTATGGA AGAGATGTCA 
GTTCCTGTTA GCTGTTGGAA AGTTAGTCAT GGTCAGTTGT TATCGGATAA TGAGTTCATC 
TACTTTTTGG CCCCTCAACA AATTGCTCAA TTTTGGACCA ACGGGTTGCA ATCTGTAGTT 
AAAAGTCTCC AAGGACAGCA AAGGTACCCT GATCGGCGAA TGTTATGGAT CAAAAATGTC 
TACTTATCAC TTTACGAGAT AACGGGCGAA TCAAACTGTG GACCACGGCC ATTTGAAGCA 
CTTCAGGCAT TTGGGCTGAG TCAAACAAAT ACTAACGCAA CACGACCCAA TGACTCGTCG 
CTGTCCAGTG AGCCAGGAGG AGCTAAAAGC AGACTGAAGA ACCTTAAAAA TGCTATGCAA 
AAGAAGCTTC GTGGTGCATC GCGCGAAGGA AGCAGATCTC AGTCACCTCA ACCTCATTCT 
CCTCTTGTCC GACCACCATC AATAAAATCG CAAATATCGT CACAATCTGG TCCGCCGGGT 
CCGAACTCTC CAGGCTATTT GCTGAAACCA CGTGGAGAAC CTGCCAATAG TGATGCAGGA 
GACATTGATA GCATTTATAC ACCTCGATCA AGAACTCCAA CAAGCAGCTC CTATGGAGGG 
CGATCCGTTG GAGGGAGATC ATGTAAATCG TGGAGATCAA GAGGAGGAGA GACACCAAAT 
TCGGGTTCCA TAAGCAGCTC GGGTCAGATG TCAATTCAAG TGTCTGGTCT GAGTGGACCA 
TCGGGAAAAG AGTTTCAGGA AAAACCATTA ACACTTGTAG AGTTCGCTGA ACTTTTTCGA 
TTGTTCAATA CAAGAATGAG AAAAGATTTG AGAGATGTGT TCAATGATGT CCTGTCTACG 
GCAACTACTC CTCAGCATTG TCCTAAAAGA GAACGTGATC GCCACAGTCC GAGAATGCAG 
TCAAGGCTGG CGAGTGTCTC CAACTCGTAC AATGCAGATT TTCTGTCTAA CGATTTCTTG 
ACTCGGAATA CGGCAGTAAC GTCCCATCAT ATCAGTGAAA AGCAAAACAA AATCTACAAT 
GCATTGGCAC TTGCGAGTGT TAATAGCATG GGCGGACTCA TGGACACTTC CCGGTCGTCG 
ATGCTGACGC CGCAAATGCT GCGCGCTTTT GTCAACACTC ATCAGATGGA GCAGATTGAC 
GAGCAAACGG CGATTAAGCT TATTCAGGAT CATGAACCTG ATGGTATCTG TCGACAAAAG 
AATCAAATGT CATTCGAAGG ATTCACTAGA TTCCTTTGCG ATCCTGTCAA CTTTGCATTC 
GTACCGGAAA CAATTGAACC AGATGAAGAG GACCTACGGT ACCCGTTGTC TCATTATTAC 
ATCAATTCAA GTCACAACAC CTATTTGACG GGACATCAAC TCAAGGGACC CAGTAGCTCG 
GAGATGTACA GACAGGTTCT CCTCACCGGG TGTCGGTGTG TCGAGCTTGA TTGTTGGGAT 
GGAGATGACG GATTGCCATT GATTTATCAT GGACACACAT TGGTTAGCAA GATTGGGTTT 
AGACAGGTGG TCGAAATTAT CAAAAAATCT GCATTCATAA CATCGGATCT TCCTGTGATA 
TTATCAATTG AGAATCATTG TTCCCTACAA CAACAGGCGA AAATGGCACA GATGTTCAAA 
ACAGTTCTTG GAGATTTACT AGTATCAAAC TTCCTTTTTG AAGCTGATTT CTCGGACTCT 
CCACGTCTTC CATGCCCTCT CCAAATGAAA AATAAGATCT TGATAAAAAA TAAGAAAATG 
ATAGTCGACC CACCAACTCC ATTGCCAATG ATTGAACGAG GAGCTGTGCA GCGTGGTGAA 
ACTCAATTAA ATCTTCACAG AAAACAGAGT AAAAACTCCT ACGAAAGCAG TACGGTAGAT 
GAAGTGGAAG ATGACGACCT AGATGAGTTT CTTGACGATG AAGAAAATGA GGAGGATGAC 
CAAGAAGAGG TTCAAGTCAG AAGTGAAAAA GAAGACAGTC CGAAGACATC AAAACGTGCA 
GAGAAATCGG CTAGAAACAT CAAACAGCAA GATTCGTTAT GTAGCGATCA TTCGGTAGAG 
CAGGCAAAAC CTTCCACAAG CAAGACCACA TCCAAAACCA ACGATAGAAA GACTGAAGAT 
GAAGTACTTT ACGCTCAACT TGCTCAAAAT GCCATCCGAA ACCAACAACC AAGAAAGAAC 
AATACTGGAG TCCAAATAGC GCCAGAGTTG TCAGATATTG TCATTTACAT GCAAGCGACA 
AAATTCAAAG GATTTCCCCC AGTCGATGGC ATTCAATCAC CAAGGATAAT GGAGGAAGGG 
CCAGCTTCTG CAAGTCTTTC ATTTTCATCA CGGGCTCGTA CGCCATCAAA TCTGCTGAAT 
ACACCAGCCC CTCCACGACG ACAGCGAAGT AGCACTCAGT TGAGTCAAGA ACTGGCTGCA 
GAATTTTTGG GATCAGTTAG GGCAAATGCT ACAGCTACAT GCTACCAGGT TACATCTCTT 
AATGAAAACG CTGCAAAGAA ACTGATGAAA AGACATCCAG CAAAATGCGT ATCTTATACT 
CGGGACCATC TGATTCGTAC GTATCCAAGT GCCAAGCACT ATGACTCGTC CAACTTTAAC 
CCCATCAACT GCTGGGCTCA TGGAATGCAA ATGGTTGCTT TAAATTTTCA AACTCCTGAT 
GTTATAATGG CAGTTAACCA AGCGATGTTC GAGCAATCTG GAAACTGTGG ATATCAACTG 
AAACCACGTT GTCTTTGGGA TGAATCTCAT CTTTTGTACA ACAAATTTTT GCCTCTCTCC 
AAAGACATTG CCGGCCATTC TGCTTTGTTA CTAAACTTAA CTATTATATC CGGACAGCAC 
GTGTATCCAA ACACCCATTA CGCCAGTTTA TATGTCGAAA TTGAAGTCAT CGGTATTCAC 
AACGACTGTG TTCGTGAGAA ATCAAAAGTG GTCCAGAGAA ATTCTGTGAA TCCAATCTGG 
AACCACACCA CTCAACTTCG AATAGCTTGT GTTGACTTAG CATTCCTTCG CATAGCAGTA 
TGTGATAGTG GGCAAAATGG AAGAGTCGTT GCTCACCGAG TTGTTCCTGT CAAATGCATT 
CGTCCGGGTT TCCGACATCT CCCACTCAGA ACTCCAACTA ATCTTCCGAT CGACAACGCT 
ATGATATTTT TAAGAACAAG ATTCGAGCAA GAAGAACACA TCTACCTCCA CGACGATGAT 
TCAAACACCT ATTGTAATCT AGAACATACG CTGGCATACC GCACAGACTT AACACCCAAT 
TTATCTCCAA CGCCAATTTT GAAGAAACAG ATCTTTGTTC TTCGAATTAC AGGCGCGTTT 
GCTGATGAAA CAGCAATTAC AGTGCACAGC GAGTCAGGAA GTACAGTGAA GACGGTGATG 
CAACAGGCGC TGCTTAACGC TGGAAAGAAT GCGGATCAGG TTGAAGAGTA TGTACTCATC 
GAAGAATCAC TGCCGGCACC TTCTGGGGAA GATCCAATTG AGCAGAGAGT ACTTCCTCTG 
AACGAGCCTA TCATGGACGC CGTGGCCTGC TGGAACGGTT CAATGCGTCG TTTTGTGTTA 
CGAAAAAAAG GCAGTGACCC ATCATCTAGA GCTTGGATCA CTTCAATTAT AAAATCCGGA 
ACAAGTGGAA GCTCCACGTC AGTCTCACCA AGTCCGTTGA CCAAAGATGG ACACGTAAAG 
AGTGCATCAA GCAATCAACT TCATGGACGA TCATTGGACA CTGACGCTTT TGGAGAGCAC 
CTTGAAGTGA CAGAAGGCAA GTGGTTGAAC CCTCGTGCAA GATCAATGGG CGACACATTT 
CTGGTATGTG TTCACAATGT TAGTGAAGAT CAGCCTTATG CAATCCTACG TGCTGGAATC 
CACAGCACTG CCGCCGACAT TATTCGTCAG GTGTTTGTTA AAGCTCGCCG TTCAAATGTA 
GACGATTCCG AGTTTGTTCT TGTTGAAGAA ACCTGCGATG ATCCAAAACT CAATCAAGGC 
CAAATGACTC CAAAGTATCC AAACAACCGC ACCACAAGCC GAGTGTTAGG ACAAAACGAA 
AATGTTTGGA AAGCACAATC GAGGTGGAAG TCAATGGGCA GATTTGTACT TGAAAACCGA 
AAGGACACAG TGCACGCAAC CCTCGAAAAA GGTAGACGTG TGGAAAGCAC AACTTCTTCC 
TCCACCACAA CGCGCAAAAT AAGTTTATCC AGCGTTCGCT CAATCGGTCT TCCACGTAAA 
TTCTCCAAGT TTGGCAAGTC GTTGACTATG GATGCTGGAC CAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024619.1
CDS	1..5688
Translation	MNWDTLKGVLKTRRLTKRTIPAYIHPTSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGKWLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKGRRVESTTSSSTTTRKISLSSVRSIGLPRKFSKFGKSLTMDAGPK

Target ORF information:

RefSeq Version	NM_001029448.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029448.4

ORF Insert Sequence:

ATGAACTGGG ACACTCTGAA AGGCGTGCTG AAAACAAGGC GTTTGACAAA ACGTACGATT 
CCAGCGTATA TTCATCCAAC ATCACGTAGT GACTCAACCT CTTCAACGCA ATCAGCCACA 
GCTGGATTCA TTCTCAATGA AGAACCAATC ACACTGTTCC GACTTGAACT CGAACGACTT 
CAATATATTC TTCATTTCCC TGAAGAAGTT GCATTCCAAC TATCTTCTAC TGAGTATCAG 
CTTTTCTACT CAATACAACC CATGGATTAT GTTCGTTACG TCAGTTGCGA TCTCACATCT 
GTTCCAGTGT CAGAAAATCC AAGTCCAGTG CGAAATCTTG TGAAAAGGCT ATCAGAGGTA 
TCATCCTGGA TAACACATGT TATTGTCAGT CAGCCAACAC ATGACGATCG AAAGGTTGCT 
CTTACTGCAA TTTTGAGAAT TGTGGAAACT TGCTGGAATA TTGGAAACTT TAATGCAGCT 
GTTGAGGTTC TTATGGGTTT AAAGAGTGAA AAACTCAGAC CATTCTGGCT TTCTTTGCGA 
CAAGAGGAAA AGTCTCAGTT TGATTCGCTT TGCGAAACCT TGCTTCCTGC AAACCAAGCT 
CTCCCATCTC AAGCTTACAT CAACGCGGTC CAACGAGCAC TGAGAATGCC ACAATCTCGT 
GTGATTCCAT TTTTTGGAAT ATTTCTTCGT GATCTCTACG CAATTGTGAA TGACTTGCCG 
AATATCGTTG TGATTGGACA AGAAGGTGAA ACCCAAAAGC TGGAATTCAT GAGCGATCCC 
AATGGCGAAG ATCATTTTTC GTCGAGAATT GGAGTGGGTG GGCTGTTGAA TGCTGATAAA 
ATCAACTTGG TGGCAATTGT TTTGGACAAC TTGGAATTAT TCCATCGTCA CAGCAGGACA 
ATGATCAAAT TGTTAGAAGA GCAAGCAGTC CCACCAATTC AAATTCCTCA GAACGAGCGG 
GAGCAAAAAG AAAAGGAAGC AAAAACATAT GAACCAGTAC AAGTGGTTCG CGGCTCTTCT 
CATGGAGTGG CTCTCATCCC TCTTGATACC CTAACATTCG ATCTTGATGT TATCCAACGA 
CTCCAGCACG GAACAACAGT GATCCATTAC GAACCTGATT CGGGAAGAAG CAATTTGTGT 
CTGCTGCGGT TAGACCCTAG TTGCGGTCAA ATAAACTGGC ATAAGATTTC ATATTCAGTT 
AACAAAGATC CCAAAGAAAA GGATGTGCTC GCCAAAGTAT CCGTTTCAAA TCTGCAGCCG 
CTAGACTCTG GCCGAGGGGC TCCGTCACCA ATGCCATCTG GAAGAACACC AGGAACTGGA 
GGAGTTGGAG TAGAGGAGGG AGAACTCAAA TTAAGTGTTG TGAAAGGAGT CGAACTAGTT 
GATAGTTATG ATATTGATAT TGAGGCAATT TACCGTCGTC ATTCTATGGA AGAGATGTCA 
GTTCCTGTTA GCTGTTGGAA AGTTAGTCAT GGTCAGTTGT TATCGGATAA TGAGTTCATC 
TACTTTTTGG CCCCTCAACA AATTGCTCAA TTTTGGACCA ACGGGTTGCA ATCTGTAGTT 
AAAAGTCTCC AAGGACAGCA AAGGTACCCT GATCGGCGAA TGTTATGGAT CAAAAATGTC 
TACTTATCAC TTTACGAGAT AACGGGCGAA TCAAACTGTG GACCACGGCC ATTTGAAGCA 
CTTCAGGCAT TTGGGCTGAG TCAAACAAAT ACTAACGCAA CACGACCCAA TGACTCGTCG 
CTGTCCAGTG AGCCAGGAGG AGCTAAAAGC AGACTGAAGA ACCTTAAAAA TGCTATGCAA 
AAGAAGCTTC GTGGTGCATC GCGCGAAGGA AGCAGATCTC AGTCACCTCA ACCTCATTCT 
CCTCTTGTCC GACCACCATC AATAAAATCG CAAATATCGT CACAATCTGG TCCGCCGGGT 
CCGAACTCTC CAGGCTATTT GCTGAAACCA CGTGGAGAAC CTGCCAATAG TGATGCAGGA 
GACATTGATA GCATTTATAC ACCTCGATCA AGAACTCCAA CAAGCAGCTC CTATGGAGGG 
CGATCCGTTG GAGGGAGATC ATGTAAATCG TGGAGATCAA GAGGAGGAGA GACACCAAAT 
TCGGGTTCCA TAAGCAGCTC GGGTCAGATG TCAATTCAAG TGTCTGGTCT GAGTGGACCA 
TCGGGAAAAG AGTTTCAGGA AAAACCATTA ACACTTGTAG AGTTCGCTGA ACTTTTTCGA 
TTGTTCAATA CAAGAATGAG AAAAGATTTG AGAGATGTGT TCAATGATGT CCTGTCTACG 
GCAACTACTC CTCAGCATTG TCCTAAAAGA GAACGTGATC GCCACAGTCC GAGAATGCAG 
TCAAGGCTGG CGAGTGTCTC CAACTCGTAC AATGCAGATT TTCTGTCTAA CGATTTCTTG 
ACTCGGAATA CGGCAGTAAC GTCCCATCAT ATCAGTGAAA AGCAAAACAA AATCTACAAT 
GCATTGGCAC TTGCGAGTGT TAATAGCATG GGCGGACTCA TGGACACTTC CCGGTCGTCG 
ATGCTGACGC CGCAAATGCT GCGCGCTTTT GTCAACACTC ATCAGATGGA GCAGATTGAC 
GAGCAAACGG CGATTAAGCT TATTCAGGAT CATGAACCTG ATGGTATCTG TCGACAAAAG 
AATCAAATGT CATTCGAAGG ATTCACTAGA TTCCTTTGCG ATCCTGTCAA CTTTGCATTC 
GTACCGGAAA CAATTGAACC AGATGAAGAG GACCTACGGT ACCCGTTGTC TCATTATTAC 
ATCAATTCAA GTCACAACAC CTATTTGACG GGACATCAAC TCAAGGGACC CAGTAGCTCG 
GAGATGTACA GACAGGTTCT CCTCACCGGG TGTCGGTGTG TCGAGCTTGA TTGTTGGGAT 
GGAGATGACG GATTGCCATT GATTTATCAT GGACACACAT TGGTTAGCAA GATTGGGTTT 
AGACAGGTGG TCGAAATTAT CAAAAAATCT GCATTCATAA CATCGGATCT TCCTGTGATA 
TTATCAATTG AGAATCATTG TTCCCTACAA CAACAGGCGA AAATGGCACA GATGTTCAAA 
ACAGTTCTTG GAGATTTACT AGTATCAAAC TTCCTTTTTG AAGCTGATTT CTCGGACTCT 
CCACGTCTTC CATGCCCTCT CCAAATGAAA AATAAGATCT TGATAAAAAA TAAGAAAATG 
ATAGTCGACC CACCAACTCC ATTGCCAATG ATTGAACGAG GAGCTGTGCA GCGTGGTGAA 
ACTCAATTAA ATCTTCACAG AAAACAGAGT AAAAACTCCT ACGAAAGCAG TACGGTAGAT 
GAAGTGGAAG ATGACGACCT AGATGAGTTT CTTGACGATG AAGAAAATGA GGAGGATGAC 
CAAGAAGAGG TTCAAGTCAG AAGTGAAAAA GAAGACAGTC CGAAGACATC AAAACGTGCA 
GAGAAATCGG CTAGAAACAT CAAACAGCAA GATTCGTTAT GTAGCGATCA TTCGGTAGAG 
CAGGCAAAAC CTTCCACAAG CAAGACCACA TCCAAAACCA ACGATAGAAA GACTGAAGAT 
GAAGTACTTT ACGCTCAACT TGCTCAAAAT GCCATCCGAA ACCAACAACC AAGAAAGAAC 
AATACTGGAG TCCAAATAGC GCCAGAGTTG TCAGATATTG TCATTTACAT GCAAGCGACA 
AAATTCAAAG GATTTCCCCC AGTCGATGGC ATTCAATCAC CAAGGATAAT GGAGGAAGGG 
CCAGCTTCTG CAAGTCTTTC ATTTTCATCA CGGGCTCGTA CGCCATCAAA TCTGCTGAAT 
ACACCAGCCC CTCCACGACG ACAGCGAAGT AGCACTCAGT TGAGTCAAGA ACTGGCTGCA 
GAATTTTTGG GATCAGTTAG GGCAAATGCT ACAGCTACAT GCTACCAGGT TACATCTCTT 
AATGAAAACG CTGCAAAGAA ACTGATGAAA AGACATCCAG CAAAATGCGT ATCTTATACT 
CGGGACCATC TGATTCGTAC GTATCCAAGT GCCAAGCACT ATGACTCGTC CAACTTTAAC 
CCCATCAACT GCTGGGCTCA TGGAATGCAA ATGGTTGCTT TAAATTTTCA AACTCCTGAT 
GTTATAATGG CAGTTAACCA AGCGATGTTC GAGCAATCTG GAAACTGTGG ATATCAACTG 
AAACCACGTT GTCTTTGGGA TGAATCTCAT CTTTTGTACA ACAAATTTTT GCCTCTCTCC 
AAAGACATTG CCGGCCATTC TGCTTTGTTA CTAAACTTAA CTATTATATC CGGACAGCAC 
GTGTATCCAA ACACCCATTA CGCCAGTTTA TATGTCGAAA TTGAAGTCAT CGGTATTCAC 
AACGACTGTG TTCGTGAGAA ATCAAAAGTG GTCCAGAGAA ATTCTGTGAA TCCAATCTGG 
AACCACACCA CTCAACTTCG AATAGCTTGT GTTGACTTAG CATTCCTTCG CATAGCAGTA 
TGTGATAGTG GGCAAAATGG AAGAGTCGTT GCTCACCGAG TTGTTCCTGT CAAATGCATT 
CGTCCGGGTT TCCGACATCT CCCACTCAGA ACTCCAACTA ATCTTCCGAT CGACAACGCT 
ATGATATTTT TAAGAACAAG ATTCGAGCAA GAAGAACACA TCTACCTCCA CGACGATGAT 
TCAAACACCT ATTGTAATCT AGAACATACG CTGGCATACC GCACAGACTT AACACCCAAT 
TTATCTCCAA CGCCAATTTT GAAGAAACAG ATCTTTGTTC TTCGAATTAC AGGCGCGTTT 
GCTGATGAAA CAGCAATTAC AGTGCACAGC GAGTCAGGAA GTACAGTGAA GACGGTGATG 
CAACAGGCGC TGCTTAACGC TGGAAAGAAT GCGGATCAGG TTGAAGAGTA TGTACTCATC 
GAAGAATCAC TGCCGGCACC TTCTGGGGAA GATCCAATTG AGCAGAGAGT ACTTCCTCTG 
AACGAGCCTA TCATGGACGC CGTGGCCTGC TGGAACGGTT CAATGCGTCG TTTTGTGTTA 
CGAAAAAAAG GCAGTGACCC ATCATCTAGA GCTTGGATCA CTTCAATTAT AAAATCCGGA 
ACAAGTGGAA GCTCCACGTC AGTCTCACCA AGTCCGTTGA CCAAAGATGG ACACGTAAAG 
AGTGCATCAA GCAATCAACT TCATGGACGA TCATTGGACA CTGACGCTTT TGGAGAGCAC 
CTTGAAGTGA CAGAAGGCAA GTGGTTGAAC CCTCGTGCAA GATCAATGGG CGACACATTT 
CTGGTATGTG TTCACAATGT TAGTGAAGAT CAGCCTTATG CAATCCTACG TGCTGGAATC 
CACAGCACTG CCGCCGACAT TATTCGTCAG GTGTTTGTTA AAGCTCGCCG TTCAAATGTA 
GACGATTCCG AGTTTGTTCT TGTTGAAGAA ACCTGCGATG ATCCAAAACT CAATCAAGGC 
CAAATGACTC CAAAGTATCC AAACAACCGC ACCACAAGCC GAGTGTTAGG ACAAAACGAA 
AATGTTTGGA AAGCACAATC GAGGTGGAAG TCAATGGGCA GATTTGTACT TGAAAACCGA 
AAGGACACAG TGCACGCAAC CCTCGAAAAA GGTAGACGTG TGGAAAGCAC AACTTCTTCC 
TCCACCACAA CGCGCAAAAT AAGTTTATCC AGCGTTCGCT CAATCGGTCT TCCACGTAAA 
TTCTCCAAGT TTGGCAAGTC GTTGACTATG GATGCTGGAC CAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_001029448.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024619.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

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CloneID	OCa348985
Clone ID Related Accession (Same CDS sequence)	NM_001029448.4 , NM_001029448.3
Accession Version	NM_001029448.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_001029448.2. COMPLETENESS: incomplete on both ends.

ATGAACTGGG ACACTCTGAA AGGCGTGCTG AAAACAAGGC GTTTGACAAA ACGTACGATT 
CCAGCGTATA TTCATCCAAC ATCACGTAGT GACTCAACCT CTTCAACGCA ATCAGCCACA 
GCTGGATTCA TTCTCAATGA AGAACCAATC ACACTGTTCC GACTTGAACT CGAACGACTT 
CAATATATTC TTCATTTCCC TGAAGAAGTT GCATTCCAAC TATCTTCTAC TGAGTATCAG 
CTTTTCTACT CAATACAACC CATGGATTAT GTTCGTTACG TCAGTTGCGA TCTCACATCT 
GTTCCAGTGT CAGAAAATCC AAGTCCAGTG CGAAATCTTG TGAAAAGGCT ATCAGAGGTA 
TCATCCTGGA TAACACATGT TATTGTCAGT CAGCCAACAC ATGACGATCG AAAGGTTGCT 
CTTACTGCAA TTTTGAGAAT TGTGGAAACT TGCTGGAATA TTGGAAACTT TAATGCAGCT 
GTTGAGGTTC TTATGGGTTT AAAGAGTGAA AAACTCAGAC CATTCTGGCT TTCTTTGCGA 
CAAGAGGAAA AGTCTCAGTT TGATTCGCTT TGCGAAACCT TGCTTCCTGC AAACCAAGCT 
CTCCCATCTC AAGCTTACAT CAACGCGGTC CAACGAGCAC TGAGAATGCC ACAATCTCGT 
GTGATTCCAT TTTTTGGAAT ATTTCTTCGT GATCTCTACG CAATTGTGAA TGACTTGCCG 
AATATCGTTG TGATTGGACA AGAAGGTGAA ACCCAAAAGC TGGAATTCAT GAGCGATCCC 
AATGGCGAAG ATCATTTTTC GTCGAGAATT GGAGTGGGTG GGCTGTTGAA TGCTGATAAA 
ATCAACTTGG TGGCAATTGT TTTGGACAAC TTGGAATTAT TCCATCGTCA CAGCAGGACA 
ATGATCAAAT TGTTAGAAGA GCAAGCAGTC CCACCAATTC AAATTCCTCA GAACGAGCGG 
GAGCAAAAAG AAAAGGAAGC AAAAACATAT GAACCAGTAC AAGTGGTTCG CGGCTCTTCT 
CATGGAGTGG CTCTCATCCC TCTTGATACC CTAACATTCG ATCTTGATGT TATCCAACGA 
CTCCAGCACG GAACAACAGT GATCCATTAC GAACCTGATT CGGGAAGAAG CAATTTGTGT 
CTGCTGCGGT TAGACCCTAG TTGCGGTCAA ATAAACTGGC ATAAGATTTC ATATTCAGTT 
AACAAAGATC CCAAAGAAAA GGATGTGCTC GCCAAAGTAT CCGTTTCAAA TCTGCAGCCG 
CTAGACTCTG GCCGAGGGGC TCCGTCACCA ATGCCATCTG GAAGAACACC AGGAACTGGA 
GGAGTTGGAG TAGAGGAGGG AGAACTCAAA TTAAGTGTTG TGAAAGGAGT CGAACTAGTT 
GATAGTTATG ATATTGATAT TGAGGCAATT TACCGTCGTC ATTCTATGGA AGAGATGTCA 
GTTCCTGTTA GCTGTTGGAA AGTTAGTCAT GGTCAGTTGT TATCGGATAA TGAGTTCATC 
TACTTTTTGG CCCCTCAACA AATTGCTCAA TTTTGGACCA ACGGGTTGCA ATCTGTAGTT 
AAAAGTCTCC AAGGACAGCA AAGGTACCCT GATCGGCGAA TGTTATGGAT CAAAAATGTC 
TACTTATCAC TTTACGAGAT AACGGGCGAA TCAAACTGTG GACCACGGCC ATTTGAAGCA 
CTTCAGGCAT TTGGGCTGAG TCAAACAAAT ACTAACGCAA CACGACCCAA TGACTCGTCG 
CTGTCCAGTG AGCCAGGAGG AGCTAAAAGC AGACTGAAGA ACCTTAAAAA TGCTATGCAA 
AAGAAGCTTC GTGGTGCATC GCGCGAAGGA AGCAGATCTC AGTCACCTCA ACCTCATTCT 
CCTCTTGTCC GACCACCATC AATAAAATCG CAAATATCGT CACAATCTGG TCCGCCGGGT 
CCGAACTCTC CAGGCTATTT GCTGAAACCA CGTGGAGAAC CTGCCAATAG TGATGCAGGA 
GACATTGATA GCATTTATAC ACCTCGATCA AGAACTCCAA CAAGCAGCTC CTATGGAGGG 
CGATCCGTTG GAGGGAGATC ATGTAAATCG TGGAGATCAA GAGGAGGAGA GACACCAAAT 
TCGGGTTCCA TAAGCAGCTC GGGTCAGATG TCAATTCAAG TGTCTGGTCT GAGTGGACCA 
TCGGGAAAAG AGTTTCAGGA AAAACCATTA ACACTTGTAG AGTTCGCTGA ACTTTTTCGA 
TTGTTCAATA CAAGAATGAG AAAAGATTTG AGAGATGTGT TCAATGATGT CCTGTCTACG 
GCAACTACTC CTCAGCATTG TCCTAAAAGA GAACGTGATC GCCACAGTCC GAGAATGCAG 
TCAAGGCTGG CGAGTGTCTC CAACTCGTAC AATGCAGATT TTCTGTCTAA CGATTTCTTG 
ACTCGGAATA CGGCAGTAAC GTCCCATCAT ATCAGTGAAA AGCAAAACAA AATCTACAAT 
GCATTGGCAC TTGCGAGTGT TAATAGCATG GGCGGACTCA TGGACACTTC CCGGTCGTCG 
ATGCTGACGC CGCAAATGCT GCGCGCTTTT GTCAACACTC ATCAGATGGA GCAGATTGAC 
GAGCAAACGG CGATTAAGCT TATTCAGGAT CATGAACCTG ATGGTATCTG TCGACAAAAG 
AATCAAATGT CATTCGAAGG ATTCACTAGA TTCCTTTGCG ATCCTGTCAA CTTTGCATTC 
GTACCGGAAA CAATTGAACC AGATGAAGAG GACCTACGGT ACCCGTTGTC TCATTATTAC 
ATCAATTCAA GTCACAACAC CTATTTGACG GGACATCAAC TCAAGGGACC CAGTAGCTCG 
GAGATGTACA GACAGGTTCT CCTCACCGGG TGTCGGTGTG TCGAGCTTGA TTGTTGGGAT 
GGAGATGACG GATTGCCATT GATTTATCAT GGACACACAT TGGTTAGCAA GATTGGGTTT 
AGACAGGTGG TCGAAATTAT CAAAAAATCT GCATTCATAA CATCGGATCT TCCTGTGATA 
TTATCAATTG AGAATCATTG TTCCCTACAA CAACAGGCGA AAATGGCACA GATGTTCAAA 
ACAGTTCTTG GAGATTTACT AGTATCAAAC TTCCTTTTTG AAGCTGATTT CTCGGACTCT 
CCACGTCTTC CATGCCCTCT CCAAATGAAA AATAAGATCT TGATAAAAAA TAAGAAAATG 
ATAGTCGACC CACCAACTCC ATTGCCAATG ATTGAACGAG GAGCTGTGCA GCGTGGTGAA 
ACTCAATTAA ATCTTCACAG AAAACAGAGT AAAAACTCCT ACGAAAGCAG TACGGTAGAT 
GAAGTGGAAG ATGACGACCT AGATGAGTTT CTTGACGATG AAGAAAATGA GGAGGATGAC 
CAAGAAGAGG TTCAAGTCAG AAGTGAAAAA GAAGACAGTC CGAAGACATC AAAACGTGCA 
GAGAAATCGG CTAGAAACAT CAAACAGCAA GATTCGTTAT GTAGCGATCA TTCGGTAGAG 
CAGGCAAAAC CTTCCACAAG CAAGACCACA TCCAAAACCA ACGATAGAAA GACTGAAGAT 
GAAGTACTTT ACGCTCAACT TGCTCAAAAT GCCATCCGAA ACCAACAACC AAGAAAGAAC 
AATACTGGAG TCCAAATAGC GCCAGAGTTG TCAGATATTG TCATTTACAT GCAAGCGACA 
AAATTCAAAG GATTTCCCCC AGTCGATGGC ATTCAATCAC CAAGGATAAT GGAGGAAGGG 
CCAGCTTCTG CAAGTCTTTC ATTTTCATCA CGGGCTCGTA CGCCATCAAA TCTGCTGAAT 
ACACCAGCCC CTCCACGACG ACAGCGAAGT AGCACTCAGT TGAGTCAAGA ACTGGCTGCA 
GAATTTTTGG GATCAGTTAG GGCAAATGCT ACAGCTACAT GCTACCAGGT TACATCTCTT 
AATGAAAACG CTGCAAAGAA ACTGATGAAA AGACATCCAG CAAAATGCGT ATCTTATACT 
CGGGACCATC TGATTCGTAC GTATCCAAGT GCCAAGCACT ATGACTCGTC CAACTTTAAC 
CCCATCAACT GCTGGGCTCA TGGAATGCAA ATGGTTGCTT TAAATTTTCA AACTCCTGAT 
GTTATAATGG CAGTTAACCA AGCGATGTTC GAGCAATCTG GAAACTGTGG ATATCAACTG 
AAACCACGTT GTCTTTGGGA TGAATCTCAT CTTTTGTACA ACAAATTTTT GCCTCTCTCC 
AAAGACATTG CCGGCCATTC TGCTTTGTTA CTAAACTTAA CTATTATATC CGGACAGCAC 
GTGTATCCAA ACACCCATTA CGCCAGTTTA TATGTCGAAA TTGAAGTCAT CGGTATTCAC 
AACGACTGTG TTCGTGAGAA ATCAAAAGTG GTCCAGAGAA ATTCTGTGAA TCCAATCTGG 
AACCACACCA CTCAACTTCG AATAGCTTGT GTTGACTTAG CATTCCTTCG CATAGCAGTA 
TGTGATAGTG GGCAAAATGG AAGAGTCGTT GCTCACCGAG TTGTTCCTGT CAAATGCATT 
CGTCCGGGTT TCCGACATCT CCCACTCAGA ACTCCAACTA ATCTTCCGAT CGACAACGCT 
ATGATATTTT TAAGAACAAG ATTCGAGCAA GAAGAACACA TCTACCTCCA CGACGATGAT 
TCAAACACCT ATTGTAATCT AGAACATACG CTGGCATACC GCACAGACTT AACACCCAAT 
TTATCTCCAA CGCCAATTTT GAAGAAACAG ATCTTTGTTC TTCGAATTAC AGGCGCGTTT 
GCTGATGAAA CAGCAATTAC AGTGCACAGC GAGTCAGGAA GTACAGTGAA GACGGTGATG 
CAACAGGCGC TGCTTAACGC TGGAAAGAAT GCGGATCAGG TTGAAGAGTA TGTACTCATC 
GAAGAATCAC TGCCGGCACC TTCTGGGGAA GATCCAATTG AGCAGAGAGT ACTTCCTCTG 
AACGAGCCTA TCATGGACGC CGTGGCCTGC TGGAACGGTT CAATGCGTCG TTTTGTGTTA 
CGAAAAAAAG GCAGTGACCC ATCATCTAGA GCTTGGATCA CTTCAATTAT AAAATCCGGA 
ACAAGTGGAA GCTCCACGTC AGTCTCACCA AGTCCGTTGA CCAAAGATGG ACACGTAAAG 
AGTGCATCAA GCAATCAACT TCATGGACGA TCATTGGACA CTGACGCTTT TGGAGAGCAC 
CTTGAAGTGA CAGAAGGCAA GTGGTTGAAC CCTCGTGCAA GATCAATGGG CGACACATTT 
CTGGTATGTG TTCACAATGT TAGTGAAGAT CAGCCTTATG CAATCCTACG TGCTGGAATC 
CACAGCACTG CCGCCGACAT TATTCGTCAG GTGTTTGTTA AAGCTCGCCG TTCAAATGTA 
GACGATTCCG AGTTTGTTCT TGTTGAAGAA ACCTGCGATG ATCCAAAACT CAATCAAGGC 
CAAATGACTC CAAAGTATCC AAACAACCGC ACCACAAGCC GAGTGTTAGG ACAAAACGAA 
AATGTTTGGA AAGCACAATC GAGGTGGAAG TCAATGGGCA GATTTGTACT TGAAAACCGA 
AAGGACACAG TGCACGCAAC CCTCGAAAAA GGTAGACGTG TGGAAAGCAC AACTTCTTCC 
TCCACCACAA CGCGCAAAAT AAGTTTATCC AGCGTTCGCT CAATCGGTCT TCCACGTAAA 
TTCTCCAAGT TTGGCAAGTC GTTGACTATG GATGCTGGAC CAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024619.1
CDS	1..5688
Translation	MNWDTLKGVLKTRRLTKRTIPAYIHPTSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGKWLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKGRRVESTTSSSTTTRKISLSSVRSIGLPRKFSKFGKSLTMDAGPK

Target ORF information:

RefSeq Version	NM_001029448.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029448.3

ORF Insert Sequence:

ATGAACTGGG ACACTCTGAA AGGCGTGCTG AAAACAAGGC GTTTGACAAA ACGTACGATT 
CCAGCGTATA TTCATCCAAC ATCACGTAGT GACTCAACCT CTTCAACGCA ATCAGCCACA 
GCTGGATTCA TTCTCAATGA AGAACCAATC ACACTGTTCC GACTTGAACT CGAACGACTT 
CAATATATTC TTCATTTCCC TGAAGAAGTT GCATTCCAAC TATCTTCTAC TGAGTATCAG 
CTTTTCTACT CAATACAACC CATGGATTAT GTTCGTTACG TCAGTTGCGA TCTCACATCT 
GTTCCAGTGT CAGAAAATCC AAGTCCAGTG CGAAATCTTG TGAAAAGGCT ATCAGAGGTA 
TCATCCTGGA TAACACATGT TATTGTCAGT CAGCCAACAC ATGACGATCG AAAGGTTGCT 
CTTACTGCAA TTTTGAGAAT TGTGGAAACT TGCTGGAATA TTGGAAACTT TAATGCAGCT 
GTTGAGGTTC TTATGGGTTT AAAGAGTGAA AAACTCAGAC CATTCTGGCT TTCTTTGCGA 
CAAGAGGAAA AGTCTCAGTT TGATTCGCTT TGCGAAACCT TGCTTCCTGC AAACCAAGCT 
CTCCCATCTC AAGCTTACAT CAACGCGGTC CAACGAGCAC TGAGAATGCC ACAATCTCGT 
GTGATTCCAT TTTTTGGAAT ATTTCTTCGT GATCTCTACG CAATTGTGAA TGACTTGCCG 
AATATCGTTG TGATTGGACA AGAAGGTGAA ACCCAAAAGC TGGAATTCAT GAGCGATCCC 
AATGGCGAAG ATCATTTTTC GTCGAGAATT GGAGTGGGTG GGCTGTTGAA TGCTGATAAA 
ATCAACTTGG TGGCAATTGT TTTGGACAAC TTGGAATTAT TCCATCGTCA CAGCAGGACA 
ATGATCAAAT TGTTAGAAGA GCAAGCAGTC CCACCAATTC AAATTCCTCA GAACGAGCGG 
GAGCAAAAAG AAAAGGAAGC AAAAACATAT GAACCAGTAC AAGTGGTTCG CGGCTCTTCT 
CATGGAGTGG CTCTCATCCC TCTTGATACC CTAACATTCG ATCTTGATGT TATCCAACGA 
CTCCAGCACG GAACAACAGT GATCCATTAC GAACCTGATT CGGGAAGAAG CAATTTGTGT 
CTGCTGCGGT TAGACCCTAG TTGCGGTCAA ATAAACTGGC ATAAGATTTC ATATTCAGTT 
AACAAAGATC CCAAAGAAAA GGATGTGCTC GCCAAAGTAT CCGTTTCAAA TCTGCAGCCG 
CTAGACTCTG GCCGAGGGGC TCCGTCACCA ATGCCATCTG GAAGAACACC AGGAACTGGA 
GGAGTTGGAG TAGAGGAGGG AGAACTCAAA TTAAGTGTTG TGAAAGGAGT CGAACTAGTT 
GATAGTTATG ATATTGATAT TGAGGCAATT TACCGTCGTC ATTCTATGGA AGAGATGTCA 
GTTCCTGTTA GCTGTTGGAA AGTTAGTCAT GGTCAGTTGT TATCGGATAA TGAGTTCATC 
TACTTTTTGG CCCCTCAACA AATTGCTCAA TTTTGGACCA ACGGGTTGCA ATCTGTAGTT 
AAAAGTCTCC AAGGACAGCA AAGGTACCCT GATCGGCGAA TGTTATGGAT CAAAAATGTC 
TACTTATCAC TTTACGAGAT AACGGGCGAA TCAAACTGTG GACCACGGCC ATTTGAAGCA 
CTTCAGGCAT TTGGGCTGAG TCAAACAAAT ACTAACGCAA CACGACCCAA TGACTCGTCG 
CTGTCCAGTG AGCCAGGAGG AGCTAAAAGC AGACTGAAGA ACCTTAAAAA TGCTATGCAA 
AAGAAGCTTC GTGGTGCATC GCGCGAAGGA AGCAGATCTC AGTCACCTCA ACCTCATTCT 
CCTCTTGTCC GACCACCATC AATAAAATCG CAAATATCGT CACAATCTGG TCCGCCGGGT 
CCGAACTCTC CAGGCTATTT GCTGAAACCA CGTGGAGAAC CTGCCAATAG TGATGCAGGA 
GACATTGATA GCATTTATAC ACCTCGATCA AGAACTCCAA CAAGCAGCTC CTATGGAGGG 
CGATCCGTTG GAGGGAGATC ATGTAAATCG TGGAGATCAA GAGGAGGAGA GACACCAAAT 
TCGGGTTCCA TAAGCAGCTC GGGTCAGATG TCAATTCAAG TGTCTGGTCT GAGTGGACCA 
TCGGGAAAAG AGTTTCAGGA AAAACCATTA ACACTTGTAG AGTTCGCTGA ACTTTTTCGA 
TTGTTCAATA CAAGAATGAG AAAAGATTTG AGAGATGTGT TCAATGATGT CCTGTCTACG 
GCAACTACTC CTCAGCATTG TCCTAAAAGA GAACGTGATC GCCACAGTCC GAGAATGCAG 
TCAAGGCTGG CGAGTGTCTC CAACTCGTAC AATGCAGATT TTCTGTCTAA CGATTTCTTG 
ACTCGGAATA CGGCAGTAAC GTCCCATCAT ATCAGTGAAA AGCAAAACAA AATCTACAAT 
GCATTGGCAC TTGCGAGTGT TAATAGCATG GGCGGACTCA TGGACACTTC CCGGTCGTCG 
ATGCTGACGC CGCAAATGCT GCGCGCTTTT GTCAACACTC ATCAGATGGA GCAGATTGAC 
GAGCAAACGG CGATTAAGCT TATTCAGGAT CATGAACCTG ATGGTATCTG TCGACAAAAG 
AATCAAATGT CATTCGAAGG ATTCACTAGA TTCCTTTGCG ATCCTGTCAA CTTTGCATTC 
GTACCGGAAA CAATTGAACC AGATGAAGAG GACCTACGGT ACCCGTTGTC TCATTATTAC 
ATCAATTCAA GTCACAACAC CTATTTGACG GGACATCAAC TCAAGGGACC CAGTAGCTCG 
GAGATGTACA GACAGGTTCT CCTCACCGGG TGTCGGTGTG TCGAGCTTGA TTGTTGGGAT 
GGAGATGACG GATTGCCATT GATTTATCAT GGACACACAT TGGTTAGCAA GATTGGGTTT 
AGACAGGTGG TCGAAATTAT CAAAAAATCT GCATTCATAA CATCGGATCT TCCTGTGATA 
TTATCAATTG AGAATCATTG TTCCCTACAA CAACAGGCGA AAATGGCACA GATGTTCAAA 
ACAGTTCTTG GAGATTTACT AGTATCAAAC TTCCTTTTTG AAGCTGATTT CTCGGACTCT 
CCACGTCTTC CATGCCCTCT CCAAATGAAA AATAAGATCT TGATAAAAAA TAAGAAAATG 
ATAGTCGACC CACCAACTCC ATTGCCAATG ATTGAACGAG GAGCTGTGCA GCGTGGTGAA 
ACTCAATTAA ATCTTCACAG AAAACAGAGT AAAAACTCCT ACGAAAGCAG TACGGTAGAT 
GAAGTGGAAG ATGACGACCT AGATGAGTTT CTTGACGATG AAGAAAATGA GGAGGATGAC 
CAAGAAGAGG TTCAAGTCAG AAGTGAAAAA GAAGACAGTC CGAAGACATC AAAACGTGCA 
GAGAAATCGG CTAGAAACAT CAAACAGCAA GATTCGTTAT GTAGCGATCA TTCGGTAGAG 
CAGGCAAAAC CTTCCACAAG CAAGACCACA TCCAAAACCA ACGATAGAAA GACTGAAGAT 
GAAGTACTTT ACGCTCAACT TGCTCAAAAT GCCATCCGAA ACCAACAACC AAGAAAGAAC 
AATACTGGAG TCCAAATAGC GCCAGAGTTG TCAGATATTG TCATTTACAT GCAAGCGACA 
AAATTCAAAG GATTTCCCCC AGTCGATGGC ATTCAATCAC CAAGGATAAT GGAGGAAGGG 
CCAGCTTCTG CAAGTCTTTC ATTTTCATCA CGGGCTCGTA CGCCATCAAA TCTGCTGAAT 
ACACCAGCCC CTCCACGACG ACAGCGAAGT AGCACTCAGT TGAGTCAAGA ACTGGCTGCA 
GAATTTTTGG GATCAGTTAG GGCAAATGCT ACAGCTACAT GCTACCAGGT TACATCTCTT 
AATGAAAACG CTGCAAAGAA ACTGATGAAA AGACATCCAG CAAAATGCGT ATCTTATACT 
CGGGACCATC TGATTCGTAC GTATCCAAGT GCCAAGCACT ATGACTCGTC CAACTTTAAC 
CCCATCAACT GCTGGGCTCA TGGAATGCAA ATGGTTGCTT TAAATTTTCA AACTCCTGAT 
GTTATAATGG CAGTTAACCA AGCGATGTTC GAGCAATCTG GAAACTGTGG ATATCAACTG 
AAACCACGTT GTCTTTGGGA TGAATCTCAT CTTTTGTACA ACAAATTTTT GCCTCTCTCC 
AAAGACATTG CCGGCCATTC TGCTTTGTTA CTAAACTTAA CTATTATATC CGGACAGCAC 
GTGTATCCAA ACACCCATTA CGCCAGTTTA TATGTCGAAA TTGAAGTCAT CGGTATTCAC 
AACGACTGTG TTCGTGAGAA ATCAAAAGTG GTCCAGAGAA ATTCTGTGAA TCCAATCTGG 
AACCACACCA CTCAACTTCG AATAGCTTGT GTTGACTTAG CATTCCTTCG CATAGCAGTA 
TGTGATAGTG GGCAAAATGG AAGAGTCGTT GCTCACCGAG TTGTTCCTGT CAAATGCATT 
CGTCCGGGTT TCCGACATCT CCCACTCAGA ACTCCAACTA ATCTTCCGAT CGACAACGCT 
ATGATATTTT TAAGAACAAG ATTCGAGCAA GAAGAACACA TCTACCTCCA CGACGATGAT 
TCAAACACCT ATTGTAATCT AGAACATACG CTGGCATACC GCACAGACTT AACACCCAAT 
TTATCTCCAA CGCCAATTTT GAAGAAACAG ATCTTTGTTC TTCGAATTAC AGGCGCGTTT 
GCTGATGAAA CAGCAATTAC AGTGCACAGC GAGTCAGGAA GTACAGTGAA GACGGTGATG 
CAACAGGCGC TGCTTAACGC TGGAAAGAAT GCGGATCAGG TTGAAGAGTA TGTACTCATC 
GAAGAATCAC TGCCGGCACC TTCTGGGGAA GATCCAATTG AGCAGAGAGT ACTTCCTCTG 
AACGAGCCTA TCATGGACGC CGTGGCCTGC TGGAACGGTT CAATGCGTCG TTTTGTGTTA 
CGAAAAAAAG GCAGTGACCC ATCATCTAGA GCTTGGATCA CTTCAATTAT AAAATCCGGA 
ACAAGTGGAA GCTCCACGTC AGTCTCACCA AGTCCGTTGA CCAAAGATGG ACACGTAAAG 
AGTGCATCAA GCAATCAACT TCATGGACGA TCATTGGACA CTGACGCTTT TGGAGAGCAC 
CTTGAAGTGA CAGAAGGCAA GTGGTTGAAC CCTCGTGCAA GATCAATGGG CGACACATTT 
CTGGTATGTG TTCACAATGT TAGTGAAGAT CAGCCTTATG CAATCCTACG TGCTGGAATC 
CACAGCACTG CCGCCGACAT TATTCGTCAG GTGTTTGTTA AAGCTCGCCG TTCAAATGTA 
GACGATTCCG AGTTTGTTCT TGTTGAAGAA ACCTGCGATG ATCCAAAACT CAATCAAGGC 
CAAATGACTC CAAAGTATCC AAACAACCGC ACCACAAGCC GAGTGTTAGG ACAAAACGAA 
AATGTTTGGA AAGCACAATC GAGGTGGAAG TCAATGGGCA GATTTGTACT TGAAAACCGA 
AAGGACACAG TGCACGCAAC CCTCGAAAAA GGTAGACGTG TGGAAAGCAC AACTTCTTCC 
TCCACCACAA CGCGCAAAAT AAGTTTATCC AGCGTTCGCT CAATCGGTCT TCCACGTAAA 
TTCTCCAAGT TTGGCAAGTC GTTGACTATG GATGCTGGAC CAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_001136454.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001129926.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

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CloneID	OCa349046
Clone ID Related Accession (Same CDS sequence)	NM_001136454.1 , NM_001136454.2
Accession Version	NM_001136454.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on both ends.

ATGAACTGGG ACACTCTGAA AGGCGTGCTG AAAACAAGGC GTTTGACAAA ACGTACGATT 
CCAGCGTATA TTCATCCAAC ATCACGTAGT GACTCAACCT CTTCAACGCA ATCAGCCACA 
GCTGGATTCA TTCTCAATGA AGAACCAATC ACACTGTTCC GACTTGAACT CGAACGACTT 
CAATATATTC TTCATTTCCC TGAAGAAGTT GCATTCCAAC TATCTTCTAC TGAGTATCAG 
CTTTTCTACT CAATACAACC CATGGATTAT GTTCGTTACG TCAGTTGCGA TCTCACATCT 
GTTCCAGTGT CAGAAAATCC AAGTCCAGTG CGAAATCTTG TGAAAAGGCT ATCAGAGGTA 
TCATCCTGGA TAACACATGT TATTGTCAGT CAGCCAACAC ATGACGATCG AAAGGTTGCT 
CTTACTGCAA TTTTGAGAAT TGTGGAAACT TGCTGGAATA TTGGAAACTT TAATGCAGCT 
GTTGAGGTTC TTATGGGTTT AAAGAGTGAA AAACTCAGAC CATTCTGGCT TTCTTTGCGA 
CAAGAGGAAA AGTCTCAGTT TGATTCGCTT TGCGAAACCT TGCTTCCTGC AAACCAAGCT 
CTCCCATCTC AAGCTTACAT CAACGCGGTC CAACGAGCAC TGAGAATGCC ACAATCTCGT 
GTGATTCCAT TTTTTGGAAT ATTTCTTCGT GATCTCTACG CAATTGTGAA TGACTTGCCG 
AATATCGTTG TGATTGGACA AGAAGGTGAA ACCCAAAAGC TGGAATTCAT GAGCGATCCC 
AATGGCGAAG ATCATTTTTC GTCGAGAATT GGAGTGGGTG GGCTGTTGAA TGCTGATAAA 
ATCAACTTGG TGGCAATTGT TTTGGACAAC TTGGAATTAT TCCATCGTCA CAGCAGGACA 
ATGATCAAAT TGTTAGAAGA GCAAGCAGTC CCACCAATTC AAATTCCTCA GAACGAGCGG 
GAGCAAAAAG AAAAGGAAGC AAAAACATAT GAACCAGTAC AAGTGGTTCG CGGCTCTTCT 
CATGGAGTGG CTCTCATCCC TCTTGATACC CTAACATTCG ATCTTGATGT TATCCAACGA 
CTCCAGCACG GAACAACAGT GATCCATTAC GAACCTGATT CGGGAAGAAG CAATTTGTGT 
CTGCTGCGGT TAGACCCTAG TTGCGGTCAA ATAAACTGGC ATAAGATTTC ATATTCAGTT 
AACAAAGATC CCAAAGAAAA GGATGTGCTC GCCAAAGTAT CCGTTTCAAA TCTGCAGCCG 
CTAGACTCTG GCCGAGGGGC TCCGTCACCA ATGCCATCTG GAAGAACACC AGGAACTGGA 
GGAGTTGGAG TAGAGGAGGG AGAACTCAAA TTAAGTGTTG TGAAAGGAGT CGAACTAGTT 
GATAGTTATG ATATTGATAT TGAGGCAATT TACCGTCGTC ATTCTATGGA AGAGATGTCA 
GTTCCTGTTA GCTGTTGGAA AGTTAGTCAT GGTCAGTTGT TATCGGATAA TGAGTTCATC 
TACTTTTTGG CCCCTCAACA AATTGCTCAA TTTTGGACCA ACGGGTTGCA ATCTGTAGTT 
AAAAGTCTCC AAGGACAGCA AAGGTACCCT GATCGGCGAA TGTTATGGAT CAAAAATGTC 
TACTTATCAC TTTACGAGAT AACGGGCGAA TCAAACTGTG GACCACGGCC ATTTGAAGCA 
CTTCAGGCAT TTGGTGGGCG GGTTGAGCGT TGGAAAGGCT TTGGGCTGAG TCAAACAAAT 
ACTAACGCAA CACGACCCAA TGACTCGTCG CTGTCCAGTG AGCCAGGAGG AGCTAAAAGC 
AGACTGAAGA ACCTTAAAAA TGCTATGCAA AAGAAGCTTC GTGGTGCATC GCGCGAAGGA 
AGCAGATCTC AGTCACCTCA ACCTCATTCT CCTCTTGTCC GACCACCATC AATAAAATCG 
CAAATATCGT CACAATCTGG TCCGCCGGGT CCGAACTCTC CAGGCTATTT GCTGAAACCA 
CGTGGAGAAC CTGCCAATAG TGATGCAGGA GACATTGATA GCATTTATAC ACCTCGATCA 
AGAACTCCAA CAAGCAGCTC CTATGGAGGG CGATCCGTTG GAGGGAGATC ATGTAAATCG 
TGGAGATCAA GAGGAGGAGA GACACCAAAT TCGGGTTCCA TAAGCAGCTC GGGTCAGATG 
TCAATTCAAG TGTCTGGTCT GAGTGGACCA TCGGGAAAAG AGTTTCAGGA AAAACCATTA 
ACACTTGTAG AGTTCGCTGA ACTTTTTCGA TTGTTCAATA CAAGAATGAG AAAAGATTTG 
AGAGATGTGT TCAATGATGT CCTGTCTACG GCAACTACTC CTCAGCATTG TCCTAAAAGA 
GAACGTGATC GCCACAGTCC GAGAATGCAG TCAAGGCTGG CGAGTGTCTC CAACTCGTAC 
AATGCAGATT TTCTGTCTAA CGATTTCTTG ACTCGGAATA CGGCAGTAAC GTCCCATCAT 
ATCAGTGAAA AGCAAAACAA AATCTACAAT GCATTGGCAC TTGCGAGTGT TAATAGCATG 
GGCGGACTCA TGGACACTTC CCGGTCGTCG ATGCTGACGC CGCAAATGCT GCGCGCTTTT 
GTCAACACTC ATCAGATGGA GCAGATTGAC GAGCAAACGG CGATTAAGCT TATTCAGGAT 
CATGAACCTG ATGGTATCTG TCGACAAAAG AATCAAATGT CATTCGAAGG ATTCACTAGA 
TTCCTTTGCG ATCCTGTCAA CTTTGCATTC GTACCGGAAA CAATTGAACC AGATGAAGAG 
GACCTACGGT ACCCGTTGTC TCATTATTAC ATCAATTCAA GTCACAACAC CTATTTGACG 
GGACATCAAC TCAAGGGACC CAGTAGCTCG GAGATGTACA GACAGGTTCT CCTCACCGGG 
TGTCGGTGTG TCGAGCTTGA TTGTTGGGAT GGAGATGACG GATTGCCATT GATTTATCAT 
GGACACACAT TGGTTAGCAA GATTGGGTTT AGACAGGTGG TCGAAATTAT CAAAAAATCT 
GCATTCATAA CATCGGATCT TCCTGTGATA TTATCAATTG AGAATCATTG TTCCCTACAA 
CAACAGGCGA AAATGGCACA GATGTTCAAA ACAGTTCTTG GAGATTTACT AGTATCAAAC 
TTCCTTTTTG AAGCTGATTT CTCGGACTCT CCACGTCTTC CATGCCCTCT CCAAATGAAA 
AATAAGATCT TGATAAAAAA TAAGAAAATG ATAGTCGACC CACCAACTCC ATTGCCAATG 
ATTGAACGAG GAGCTGTGCA GCGTGGTGAA ACTCAATTAA ATCTTCACAG AAAACAGAGT 
AAAAACTCCT ACGAAAGCAG TACGGTAGAT GAAGTGGAAG ATGACGACCT AGATGAGTTT 
CTTGACGATG AAGAAAATGA GGAGGATGAC CAAGAAGAGG TTCAAGTCAG AAGTGAAAAA 
GAAGACAGTC CGAAGACATC AAAACGTGCA GAGAAATCGG CTAGAAACAT CAAACAGCAA 
GATTCGTTAT GTAGCGATCA TTCGGTAGAG CAGGCAAAAC CTTCCACAAG CAAGACCACA 
TCCAAAACCA ACGATAGAAA GACTGAAGAT GAAGTACTTT ACGCTCAACT TGCTCAAAAT 
GCCATCCGAA ACCAACAACC AAGAAAGAAC AATACTGGAG TCCAAATAGC GCCAGAGTTG 
TCAGATATTG TCATTTACAT GCAAGCGACA AAATTCAAAG GATTTCCCCC AGTCGATGGC 
ATTCAATCAC CAAGGATAAT GGAGGAAGGG CCAGCTTCTG CAAGTCTTTC ATTTTCATCA 
CGGGCTCGTA CGCCATCAAA TCTGCTGAAT ACACCAGCCC CTCCACGACG ACAGCGAAGT 
AGCACTCAGT TGAGTCAAGA ACTGGCTGCA GAATTTTTGG GATCAGTTAG GGCAAATGCT 
ACAGCTACAT GCTACCAGGT TACATCTCTT AATGAAAACG CTGCAAAGAA ACTGATGAAA 
AGACATCCAG CAAAATGCGT ATCTTATACT CGGGACCATC TGATTCGTAC GTATCCAAGT 
GCCAAGCACT ATGACTCGTC CAACTTTAAC CCCATCAACT GCTGGGCTCA TGGAATGCAA 
ATGGTTGCTT TAAATTTTCA AACTCCTGAT GTTATAATGG CAGTTAACCA AGCGATGTTC 
GAGCAATCTG GAAACTGTGG ATATCAACTG AAACCACGTT GTCTTTGGGA TGAATCTCAT 
CTTTTGTACA ACAAATTTTT GCCTCTCTCC AAAGACATTG CCGGCCATTC TGCTTTGTTA 
CTAAACTTAA CTATTATATC CGGACAGCAC GTGTATCCAA ACACCCATTA CGCCAGTTTA 
TATGTCGAAA TTGAAGTCAT CGGTATTCAC AACGACTGTG TTCGTGAGAA ATCAAAAGTG 
GTCCAGAGAA ATTCTGTGAA TCCAATCTGG AACCACACCA CTCAACTTCG AATAGCTTGT 
GTTGACTTAG CATTCCTTCG CATAGCAGTA TGTGATAGTG GGCAAAATGG AAGAGTCGTT 
GCTCACCGAG TTGTTCCTGT CAAATGCATT CGTCCGGGTT TCCGACATCT CCCACTCAGA 
ACTCCAACTA ATCTTCCGAT CGACAACGCT ATGATATTTT TAAGAACAAG ATTCGAGCAA 
GAAGAACACA TCTACCTCCA CGACGATGAT TCAAACACCT ATTGTAATCT AGAACATACG 
CTGGCATACC GCACAGACTT AACACCCAAT TTATCTCCAA CGCCAATTTT GAAGAAACAG 
ATCTTTGTTC TTCGAATTAC AGGCGCGTTT GCTGATGAAA CAGCAATTAC AGTGCACAGC 
GAGTCAGGAA GTACAGTGAA GACGGTGATG CAACAGGCGC TGCTTAACGC TGGAAAGAAT 
GCGGATCAGG TTGAAGAGTA TGTACTCATC GAAGAATCAC TGCCGGCACC TTCTGGGGAA 
GATCCAATTG AGCAGAGAGT ACTTCCTCTG AACGAGCCTA TCATGGACGC CGTGGCCTGC 
TGGAACGGTT CAATGCGTCG TTTTGTGTTA CGAAAAAAAG GCAGTGACCC ATCATCTAGA 
GCTTGGATCA CTTCAATTAT AAAATCCGGA ACAAGTGGAA GCTCCACGTC AGTCTCACCA 
AGTCCGTTGA CCAAAGATGG ACACGTAAAG AGTGCATCAA GCAATCAACT TCATGGACGA 
TCATTGGACA CTGACGCTTT TGGAGAGCAC CTTGAAGTGA CAGAAGGGTT GAACCCTCGT 
GCAAGATCAA TGGGCGACAC ATTTCTGGTA TGTGTTCACA ATGTTAGTGA AGATCAGCCT 
TATGCAATCC TACGTGCTGG AATCCACAGC ACTGCCGCCG ACATTATTCG TCAGGTGTTT 
GTTAAAGCTC GCCGTTCAAA TGTAGACGAT TCCGAGTTTG TTCTTGTTGA AGAAACCTGC 
GATGATCCAA AACTCAATCA AGGCCAAATG ACTCCAAAGT ATCCAAACAA CCGCACCACA 
AGCCGAGTGT TAGGACAAAA CGAAAATGTT TGGAAAGCAC AATCGAGGTG GAAGTCAATG 
GGCAGATTTG TACTTGAAAA CCGAAAGGAC ACAGTGCACG CAACCCTCGA AAAAGTGAGA 
TCATTTATAT CAAAACTGGA AGCTGCAAAA GCAAGCATGG ATCCCAGACA CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129926.1
CDS	1..5637
Translation	MNWDTLKGVLKTRRLTKRTIPAYIHPTSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGGRVERWKGFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKVRSFISKLEAAKASMDPRHE

Target ORF information:

RefSeq Version	NM_001136454.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136454.1

ORF Insert Sequence:

ATGAACTGGG ACACTCTGAA AGGCGTGCTG AAAACAAGGC GTTTGACAAA ACGTACGATT 
CCAGCGTATA TTCATCCAAC ATCACGTAGT GACTCAACCT CTTCAACGCA ATCAGCCACA 
GCTGGATTCA TTCTCAATGA AGAACCAATC ACACTGTTCC GACTTGAACT CGAACGACTT 
CAATATATTC TTCATTTCCC TGAAGAAGTT GCATTCCAAC TATCTTCTAC TGAGTATCAG 
CTTTTCTACT CAATACAACC CATGGATTAT GTTCGTTACG TCAGTTGCGA TCTCACATCT 
GTTCCAGTGT CAGAAAATCC AAGTCCAGTG CGAAATCTTG TGAAAAGGCT ATCAGAGGTA 
TCATCCTGGA TAACACATGT TATTGTCAGT CAGCCAACAC ATGACGATCG AAAGGTTGCT 
CTTACTGCAA TTTTGAGAAT TGTGGAAACT TGCTGGAATA TTGGAAACTT TAATGCAGCT 
GTTGAGGTTC TTATGGGTTT AAAGAGTGAA AAACTCAGAC CATTCTGGCT TTCTTTGCGA 
CAAGAGGAAA AGTCTCAGTT TGATTCGCTT TGCGAAACCT TGCTTCCTGC AAACCAAGCT 
CTCCCATCTC AAGCTTACAT CAACGCGGTC CAACGAGCAC TGAGAATGCC ACAATCTCGT 
GTGATTCCAT TTTTTGGAAT ATTTCTTCGT GATCTCTACG CAATTGTGAA TGACTTGCCG 
AATATCGTTG TGATTGGACA AGAAGGTGAA ACCCAAAAGC TGGAATTCAT GAGCGATCCC 
AATGGCGAAG ATCATTTTTC GTCGAGAATT GGAGTGGGTG GGCTGTTGAA TGCTGATAAA 
ATCAACTTGG TGGCAATTGT TTTGGACAAC TTGGAATTAT TCCATCGTCA CAGCAGGACA 
ATGATCAAAT TGTTAGAAGA GCAAGCAGTC CCACCAATTC AAATTCCTCA GAACGAGCGG 
GAGCAAAAAG AAAAGGAAGC AAAAACATAT GAACCAGTAC AAGTGGTTCG CGGCTCTTCT 
CATGGAGTGG CTCTCATCCC TCTTGATACC CTAACATTCG ATCTTGATGT TATCCAACGA 
CTCCAGCACG GAACAACAGT GATCCATTAC GAACCTGATT CGGGAAGAAG CAATTTGTGT 
CTGCTGCGGT TAGACCCTAG TTGCGGTCAA ATAAACTGGC ATAAGATTTC ATATTCAGTT 
AACAAAGATC CCAAAGAAAA GGATGTGCTC GCCAAAGTAT CCGTTTCAAA TCTGCAGCCG 
CTAGACTCTG GCCGAGGGGC TCCGTCACCA ATGCCATCTG GAAGAACACC AGGAACTGGA 
GGAGTTGGAG TAGAGGAGGG AGAACTCAAA TTAAGTGTTG TGAAAGGAGT CGAACTAGTT 
GATAGTTATG ATATTGATAT TGAGGCAATT TACCGTCGTC ATTCTATGGA AGAGATGTCA 
GTTCCTGTTA GCTGTTGGAA AGTTAGTCAT GGTCAGTTGT TATCGGATAA TGAGTTCATC 
TACTTTTTGG CCCCTCAACA AATTGCTCAA TTTTGGACCA ACGGGTTGCA ATCTGTAGTT 
AAAAGTCTCC AAGGACAGCA AAGGTACCCT GATCGGCGAA TGTTATGGAT CAAAAATGTC 
TACTTATCAC TTTACGAGAT AACGGGCGAA TCAAACTGTG GACCACGGCC ATTTGAAGCA 
CTTCAGGCAT TTGGTGGGCG GGTTGAGCGT TGGAAAGGCT TTGGGCTGAG TCAAACAAAT 
ACTAACGCAA CACGACCCAA TGACTCGTCG CTGTCCAGTG AGCCAGGAGG AGCTAAAAGC 
AGACTGAAGA ACCTTAAAAA TGCTATGCAA AAGAAGCTTC GTGGTGCATC GCGCGAAGGA 
AGCAGATCTC AGTCACCTCA ACCTCATTCT CCTCTTGTCC GACCACCATC AATAAAATCG 
CAAATATCGT CACAATCTGG TCCGCCGGGT CCGAACTCTC CAGGCTATTT GCTGAAACCA 
CGTGGAGAAC CTGCCAATAG TGATGCAGGA GACATTGATA GCATTTATAC ACCTCGATCA 
AGAACTCCAA CAAGCAGCTC CTATGGAGGG CGATCCGTTG GAGGGAGATC ATGTAAATCG 
TGGAGATCAA GAGGAGGAGA GACACCAAAT TCGGGTTCCA TAAGCAGCTC GGGTCAGATG 
TCAATTCAAG TGTCTGGTCT GAGTGGACCA TCGGGAAAAG AGTTTCAGGA AAAACCATTA 
ACACTTGTAG AGTTCGCTGA ACTTTTTCGA TTGTTCAATA CAAGAATGAG AAAAGATTTG 
AGAGATGTGT TCAATGATGT CCTGTCTACG GCAACTACTC CTCAGCATTG TCCTAAAAGA 
GAACGTGATC GCCACAGTCC GAGAATGCAG TCAAGGCTGG CGAGTGTCTC CAACTCGTAC 
AATGCAGATT TTCTGTCTAA CGATTTCTTG ACTCGGAATA CGGCAGTAAC GTCCCATCAT 
ATCAGTGAAA AGCAAAACAA AATCTACAAT GCATTGGCAC TTGCGAGTGT TAATAGCATG 
GGCGGACTCA TGGACACTTC CCGGTCGTCG ATGCTGACGC CGCAAATGCT GCGCGCTTTT 
GTCAACACTC ATCAGATGGA GCAGATTGAC GAGCAAACGG CGATTAAGCT TATTCAGGAT 
CATGAACCTG ATGGTATCTG TCGACAAAAG AATCAAATGT CATTCGAAGG ATTCACTAGA 
TTCCTTTGCG ATCCTGTCAA CTTTGCATTC GTACCGGAAA CAATTGAACC AGATGAAGAG 
GACCTACGGT ACCCGTTGTC TCATTATTAC ATCAATTCAA GTCACAACAC CTATTTGACG 
GGACATCAAC TCAAGGGACC CAGTAGCTCG GAGATGTACA GACAGGTTCT CCTCACCGGG 
TGTCGGTGTG TCGAGCTTGA TTGTTGGGAT GGAGATGACG GATTGCCATT GATTTATCAT 
GGACACACAT TGGTTAGCAA GATTGGGTTT AGACAGGTGG TCGAAATTAT CAAAAAATCT 
GCATTCATAA CATCGGATCT TCCTGTGATA TTATCAATTG AGAATCATTG TTCCCTACAA 
CAACAGGCGA AAATGGCACA GATGTTCAAA ACAGTTCTTG GAGATTTACT AGTATCAAAC 
TTCCTTTTTG AAGCTGATTT CTCGGACTCT CCACGTCTTC CATGCCCTCT CCAAATGAAA 
AATAAGATCT TGATAAAAAA TAAGAAAATG ATAGTCGACC CACCAACTCC ATTGCCAATG 
ATTGAACGAG GAGCTGTGCA GCGTGGTGAA ACTCAATTAA ATCTTCACAG AAAACAGAGT 
AAAAACTCCT ACGAAAGCAG TACGGTAGAT GAAGTGGAAG ATGACGACCT AGATGAGTTT 
CTTGACGATG AAGAAAATGA GGAGGATGAC CAAGAAGAGG TTCAAGTCAG AAGTGAAAAA 
GAAGACAGTC CGAAGACATC AAAACGTGCA GAGAAATCGG CTAGAAACAT CAAACAGCAA 
GATTCGTTAT GTAGCGATCA TTCGGTAGAG CAGGCAAAAC CTTCCACAAG CAAGACCACA 
TCCAAAACCA ACGATAGAAA GACTGAAGAT GAAGTACTTT ACGCTCAACT TGCTCAAAAT 
GCCATCCGAA ACCAACAACC AAGAAAGAAC AATACTGGAG TCCAAATAGC GCCAGAGTTG 
TCAGATATTG TCATTTACAT GCAAGCGACA AAATTCAAAG GATTTCCCCC AGTCGATGGC 
ATTCAATCAC CAAGGATAAT GGAGGAAGGG CCAGCTTCTG CAAGTCTTTC ATTTTCATCA 
CGGGCTCGTA CGCCATCAAA TCTGCTGAAT ACACCAGCCC CTCCACGACG ACAGCGAAGT 
AGCACTCAGT TGAGTCAAGA ACTGGCTGCA GAATTTTTGG GATCAGTTAG GGCAAATGCT 
ACAGCTACAT GCTACCAGGT TACATCTCTT AATGAAAACG CTGCAAAGAA ACTGATGAAA 
AGACATCCAG CAAAATGCGT ATCTTATACT CGGGACCATC TGATTCGTAC GTATCCAAGT 
GCCAAGCACT ATGACTCGTC CAACTTTAAC CCCATCAACT GCTGGGCTCA TGGAATGCAA 
ATGGTTGCTT TAAATTTTCA AACTCCTGAT GTTATAATGG CAGTTAACCA AGCGATGTTC 
GAGCAATCTG GAAACTGTGG ATATCAACTG AAACCACGTT GTCTTTGGGA TGAATCTCAT 
CTTTTGTACA ACAAATTTTT GCCTCTCTCC AAAGACATTG CCGGCCATTC TGCTTTGTTA 
CTAAACTTAA CTATTATATC CGGACAGCAC GTGTATCCAA ACACCCATTA CGCCAGTTTA 
TATGTCGAAA TTGAAGTCAT CGGTATTCAC AACGACTGTG TTCGTGAGAA ATCAAAAGTG 
GTCCAGAGAA ATTCTGTGAA TCCAATCTGG AACCACACCA CTCAACTTCG AATAGCTTGT 
GTTGACTTAG CATTCCTTCG CATAGCAGTA TGTGATAGTG GGCAAAATGG AAGAGTCGTT 
GCTCACCGAG TTGTTCCTGT CAAATGCATT CGTCCGGGTT TCCGACATCT CCCACTCAGA 
ACTCCAACTA ATCTTCCGAT CGACAACGCT ATGATATTTT TAAGAACAAG ATTCGAGCAA 
GAAGAACACA TCTACCTCCA CGACGATGAT TCAAACACCT ATTGTAATCT AGAACATACG 
CTGGCATACC GCACAGACTT AACACCCAAT TTATCTCCAA CGCCAATTTT GAAGAAACAG 
ATCTTTGTTC TTCGAATTAC AGGCGCGTTT GCTGATGAAA CAGCAATTAC AGTGCACAGC 
GAGTCAGGAA GTACAGTGAA GACGGTGATG CAACAGGCGC TGCTTAACGC TGGAAAGAAT 
GCGGATCAGG TTGAAGAGTA TGTACTCATC GAAGAATCAC TGCCGGCACC TTCTGGGGAA 
GATCCAATTG AGCAGAGAGT ACTTCCTCTG AACGAGCCTA TCATGGACGC CGTGGCCTGC 
TGGAACGGTT CAATGCGTCG TTTTGTGTTA CGAAAAAAAG GCAGTGACCC ATCATCTAGA 
GCTTGGATCA CTTCAATTAT AAAATCCGGA ACAAGTGGAA GCTCCACGTC AGTCTCACCA 
AGTCCGTTGA CCAAAGATGG ACACGTAAAG AGTGCATCAA GCAATCAACT TCATGGACGA 
TCATTGGACA CTGACGCTTT TGGAGAGCAC CTTGAAGTGA CAGAAGGGTT GAACCCTCGT 
GCAAGATCAA TGGGCGACAC ATTTCTGGTA TGTGTTCACA ATGTTAGTGA AGATCAGCCT 
TATGCAATCC TACGTGCTGG AATCCACAGC ACTGCCGCCG ACATTATTCG TCAGGTGTTT 
GTTAAAGCTC GCCGTTCAAA TGTAGACGAT TCCGAGTTTG TTCTTGTTGA AGAAACCTGC 
GATGATCCAA AACTCAATCA AGGCCAAATG ACTCCAAAGT ATCCAAACAA CCGCACCACA 
AGCCGAGTGT TAGGACAAAA CGAAAATGTT TGGAAAGCAC AATCGAGGTG GAAGTCAATG 
GGCAGATTTG TACTTGAAAA CCGAAAGGAC ACAGTGCACG CAACCCTCGA AAAAGTGAGA 
TCATTTATAT CAAAACTGGA AGCTGCAAAA GCAAGCATGG ATCCCAGACA CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_001136454.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001129926.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

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CloneID	OCa349046
Clone ID Related Accession (Same CDS sequence)	NM_001136454.1 , NM_001136454.2
Accession Version	NM_001136454.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_001136454.1. COMPLETENESS: incomplete on the 5' end.

ATGAACTGGG ACACTCTGAA AGGCGTGCTG AAAACAAGGC GTTTGACAAA ACGTACGATT 
CCAGCGTATA TTCATCCAAC ATCACGTAGT GACTCAACCT CTTCAACGCA ATCAGCCACA 
GCTGGATTCA TTCTCAATGA AGAACCAATC ACACTGTTCC GACTTGAACT CGAACGACTT 
CAATATATTC TTCATTTCCC TGAAGAAGTT GCATTCCAAC TATCTTCTAC TGAGTATCAG 
CTTTTCTACT CAATACAACC CATGGATTAT GTTCGTTACG TCAGTTGCGA TCTCACATCT 
GTTCCAGTGT CAGAAAATCC AAGTCCAGTG CGAAATCTTG TGAAAAGGCT ATCAGAGGTA 
TCATCCTGGA TAACACATGT TATTGTCAGT CAGCCAACAC ATGACGATCG AAAGGTTGCT 
CTTACTGCAA TTTTGAGAAT TGTGGAAACT TGCTGGAATA TTGGAAACTT TAATGCAGCT 
GTTGAGGTTC TTATGGGTTT AAAGAGTGAA AAACTCAGAC CATTCTGGCT TTCTTTGCGA 
CAAGAGGAAA AGTCTCAGTT TGATTCGCTT TGCGAAACCT TGCTTCCTGC AAACCAAGCT 
CTCCCATCTC AAGCTTACAT CAACGCGGTC CAACGAGCAC TGAGAATGCC ACAATCTCGT 
GTGATTCCAT TTTTTGGAAT ATTTCTTCGT GATCTCTACG CAATTGTGAA TGACTTGCCG 
AATATCGTTG TGATTGGACA AGAAGGTGAA ACCCAAAAGC TGGAATTCAT GAGCGATCCC 
AATGGCGAAG ATCATTTTTC GTCGAGAATT GGAGTGGGTG GGCTGTTGAA TGCTGATAAA 
ATCAACTTGG TGGCAATTGT TTTGGACAAC TTGGAATTAT TCCATCGTCA CAGCAGGACA 
ATGATCAAAT TGTTAGAAGA GCAAGCAGTC CCACCAATTC AAATTCCTCA GAACGAGCGG 
GAGCAAAAAG AAAAGGAAGC AAAAACATAT GAACCAGTAC AAGTGGTTCG CGGCTCTTCT 
CATGGAGTGG CTCTCATCCC TCTTGATACC CTAACATTCG ATCTTGATGT TATCCAACGA 
CTCCAGCACG GAACAACAGT GATCCATTAC GAACCTGATT CGGGAAGAAG CAATTTGTGT 
CTGCTGCGGT TAGACCCTAG TTGCGGTCAA ATAAACTGGC ATAAGATTTC ATATTCAGTT 
AACAAAGATC CCAAAGAAAA GGATGTGCTC GCCAAAGTAT CCGTTTCAAA TCTGCAGCCG 
CTAGACTCTG GCCGAGGGGC TCCGTCACCA ATGCCATCTG GAAGAACACC AGGAACTGGA 
GGAGTTGGAG TAGAGGAGGG AGAACTCAAA TTAAGTGTTG TGAAAGGAGT CGAACTAGTT 
GATAGTTATG ATATTGATAT TGAGGCAATT TACCGTCGTC ATTCTATGGA AGAGATGTCA 
GTTCCTGTTA GCTGTTGGAA AGTTAGTCAT GGTCAGTTGT TATCGGATAA TGAGTTCATC 
TACTTTTTGG CCCCTCAACA AATTGCTCAA TTTTGGACCA ACGGGTTGCA ATCTGTAGTT 
AAAAGTCTCC AAGGACAGCA AAGGTACCCT GATCGGCGAA TGTTATGGAT CAAAAATGTC 
TACTTATCAC TTTACGAGAT AACGGGCGAA TCAAACTGTG GACCACGGCC ATTTGAAGCA 
CTTCAGGCAT TTGGTGGGCG GGTTGAGCGT TGGAAAGGCT TTGGGCTGAG TCAAACAAAT 
ACTAACGCAA CACGACCCAA TGACTCGTCG CTGTCCAGTG AGCCAGGAGG AGCTAAAAGC 
AGACTGAAGA ACCTTAAAAA TGCTATGCAA AAGAAGCTTC GTGGTGCATC GCGCGAAGGA 
AGCAGATCTC AGTCACCTCA ACCTCATTCT CCTCTTGTCC GACCACCATC AATAAAATCG 
CAAATATCGT CACAATCTGG TCCGCCGGGT CCGAACTCTC CAGGCTATTT GCTGAAACCA 
CGTGGAGAAC CTGCCAATAG TGATGCAGGA GACATTGATA GCATTTATAC ACCTCGATCA 
AGAACTCCAA CAAGCAGCTC CTATGGAGGG CGATCCGTTG GAGGGAGATC ATGTAAATCG 
TGGAGATCAA GAGGAGGAGA GACACCAAAT TCGGGTTCCA TAAGCAGCTC GGGTCAGATG 
TCAATTCAAG TGTCTGGTCT GAGTGGACCA TCGGGAAAAG AGTTTCAGGA AAAACCATTA 
ACACTTGTAG AGTTCGCTGA ACTTTTTCGA TTGTTCAATA CAAGAATGAG AAAAGATTTG 
AGAGATGTGT TCAATGATGT CCTGTCTACG GCAACTACTC CTCAGCATTG TCCTAAAAGA 
GAACGTGATC GCCACAGTCC GAGAATGCAG TCAAGGCTGG CGAGTGTCTC CAACTCGTAC 
AATGCAGATT TTCTGTCTAA CGATTTCTTG ACTCGGAATA CGGCAGTAAC GTCCCATCAT 
ATCAGTGAAA AGCAAAACAA AATCTACAAT GCATTGGCAC TTGCGAGTGT TAATAGCATG 
GGCGGACTCA TGGACACTTC CCGGTCGTCG ATGCTGACGC CGCAAATGCT GCGCGCTTTT 
GTCAACACTC ATCAGATGGA GCAGATTGAC GAGCAAACGG CGATTAAGCT TATTCAGGAT 
CATGAACCTG ATGGTATCTG TCGACAAAAG AATCAAATGT CATTCGAAGG ATTCACTAGA 
TTCCTTTGCG ATCCTGTCAA CTTTGCATTC GTACCGGAAA CAATTGAACC AGATGAAGAG 
GACCTACGGT ACCCGTTGTC TCATTATTAC ATCAATTCAA GTCACAACAC CTATTTGACG 
GGACATCAAC TCAAGGGACC CAGTAGCTCG GAGATGTACA GACAGGTTCT CCTCACCGGG 
TGTCGGTGTG TCGAGCTTGA TTGTTGGGAT GGAGATGACG GATTGCCATT GATTTATCAT 
GGACACACAT TGGTTAGCAA GATTGGGTTT AGACAGGTGG TCGAAATTAT CAAAAAATCT 
GCATTCATAA CATCGGATCT TCCTGTGATA TTATCAATTG AGAATCATTG TTCCCTACAA 
CAACAGGCGA AAATGGCACA GATGTTCAAA ACAGTTCTTG GAGATTTACT AGTATCAAAC 
TTCCTTTTTG AAGCTGATTT CTCGGACTCT CCACGTCTTC CATGCCCTCT CCAAATGAAA 
AATAAGATCT TGATAAAAAA TAAGAAAATG ATAGTCGACC CACCAACTCC ATTGCCAATG 
ATTGAACGAG GAGCTGTGCA GCGTGGTGAA ACTCAATTAA ATCTTCACAG AAAACAGAGT 
AAAAACTCCT ACGAAAGCAG TACGGTAGAT GAAGTGGAAG ATGACGACCT AGATGAGTTT 
CTTGACGATG AAGAAAATGA GGAGGATGAC CAAGAAGAGG TTCAAGTCAG AAGTGAAAAA 
GAAGACAGTC CGAAGACATC AAAACGTGCA GAGAAATCGG CTAGAAACAT CAAACAGCAA 
GATTCGTTAT GTAGCGATCA TTCGGTAGAG CAGGCAAAAC CTTCCACAAG CAAGACCACA 
TCCAAAACCA ACGATAGAAA GACTGAAGAT GAAGTACTTT ACGCTCAACT TGCTCAAAAT 
GCCATCCGAA ACCAACAACC AAGAAAGAAC AATACTGGAG TCCAAATAGC GCCAGAGTTG 
TCAGATATTG TCATTTACAT GCAAGCGACA AAATTCAAAG GATTTCCCCC AGTCGATGGC 
ATTCAATCAC CAAGGATAAT GGAGGAAGGG CCAGCTTCTG CAAGTCTTTC ATTTTCATCA 
CGGGCTCGTA CGCCATCAAA TCTGCTGAAT ACACCAGCCC CTCCACGACG ACAGCGAAGT 
AGCACTCAGT TGAGTCAAGA ACTGGCTGCA GAATTTTTGG GATCAGTTAG GGCAAATGCT 
ACAGCTACAT GCTACCAGGT TACATCTCTT AATGAAAACG CTGCAAAGAA ACTGATGAAA 
AGACATCCAG CAAAATGCGT ATCTTATACT CGGGACCATC TGATTCGTAC GTATCCAAGT 
GCCAAGCACT ATGACTCGTC CAACTTTAAC CCCATCAACT GCTGGGCTCA TGGAATGCAA 
ATGGTTGCTT TAAATTTTCA AACTCCTGAT GTTATAATGG CAGTTAACCA AGCGATGTTC 
GAGCAATCTG GAAACTGTGG ATATCAACTG AAACCACGTT GTCTTTGGGA TGAATCTCAT 
CTTTTGTACA ACAAATTTTT GCCTCTCTCC AAAGACATTG CCGGCCATTC TGCTTTGTTA 
CTAAACTTAA CTATTATATC CGGACAGCAC GTGTATCCAA ACACCCATTA CGCCAGTTTA 
TATGTCGAAA TTGAAGTCAT CGGTATTCAC AACGACTGTG TTCGTGAGAA ATCAAAAGTG 
GTCCAGAGAA ATTCTGTGAA TCCAATCTGG AACCACACCA CTCAACTTCG AATAGCTTGT 
GTTGACTTAG CATTCCTTCG CATAGCAGTA TGTGATAGTG GGCAAAATGG AAGAGTCGTT 
GCTCACCGAG TTGTTCCTGT CAAATGCATT CGTCCGGGTT TCCGACATCT CCCACTCAGA 
ACTCCAACTA ATCTTCCGAT CGACAACGCT ATGATATTTT TAAGAACAAG ATTCGAGCAA 
GAAGAACACA TCTACCTCCA CGACGATGAT TCAAACACCT ATTGTAATCT AGAACATACG 
CTGGCATACC GCACAGACTT AACACCCAAT TTATCTCCAA CGCCAATTTT GAAGAAACAG 
ATCTTTGTTC TTCGAATTAC AGGCGCGTTT GCTGATGAAA CAGCAATTAC AGTGCACAGC 
GAGTCAGGAA GTACAGTGAA GACGGTGATG CAACAGGCGC TGCTTAACGC TGGAAAGAAT 
GCGGATCAGG TTGAAGAGTA TGTACTCATC GAAGAATCAC TGCCGGCACC TTCTGGGGAA 
GATCCAATTG AGCAGAGAGT ACTTCCTCTG AACGAGCCTA TCATGGACGC CGTGGCCTGC 
TGGAACGGTT CAATGCGTCG TTTTGTGTTA CGAAAAAAAG GCAGTGACCC ATCATCTAGA 
GCTTGGATCA CTTCAATTAT AAAATCCGGA ACAAGTGGAA GCTCCACGTC AGTCTCACCA 
AGTCCGTTGA CCAAAGATGG ACACGTAAAG AGTGCATCAA GCAATCAACT TCATGGACGA 
TCATTGGACA CTGACGCTTT TGGAGAGCAC CTTGAAGTGA CAGAAGGGTT GAACCCTCGT 
GCAAGATCAA TGGGCGACAC ATTTCTGGTA TGTGTTCACA ATGTTAGTGA AGATCAGCCT 
TATGCAATCC TACGTGCTGG AATCCACAGC ACTGCCGCCG ACATTATTCG TCAGGTGTTT 
GTTAAAGCTC GCCGTTCAAA TGTAGACGAT TCCGAGTTTG TTCTTGTTGA AGAAACCTGC 
GATGATCCAA AACTCAATCA AGGCCAAATG ACTCCAAAGT ATCCAAACAA CCGCACCACA 
AGCCGAGTGT TAGGACAAAA CGAAAATGTT TGGAAAGCAC AATCGAGGTG GAAGTCAATG 
GGCAGATTTG TACTTGAAAA CCGAAAGGAC ACAGTGCACG CAACCCTCGA AAAAGTGAGA 
TCATTTATAT CAAAACTGGA AGCTGCAAAA GCAAGCATGG ATCCCAGACA CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129926.1
CDS	1..5637
Translation	MNWDTLKGVLKTRRLTKRTIPAYIHPTSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGGRVERWKGFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKVRSFISKLEAAKASMDPRHE

Target ORF information:

RefSeq Version	NM_001136454.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136454.2

ORF Insert Sequence:

ATGAACTGGG ACACTCTGAA AGGCGTGCTG AAAACAAGGC GTTTGACAAA ACGTACGATT 
CCAGCGTATA TTCATCCAAC ATCACGTAGT GACTCAACCT CTTCAACGCA ATCAGCCACA 
GCTGGATTCA TTCTCAATGA AGAACCAATC ACACTGTTCC GACTTGAACT CGAACGACTT 
CAATATATTC TTCATTTCCC TGAAGAAGTT GCATTCCAAC TATCTTCTAC TGAGTATCAG 
CTTTTCTACT CAATACAACC CATGGATTAT GTTCGTTACG TCAGTTGCGA TCTCACATCT 
GTTCCAGTGT CAGAAAATCC AAGTCCAGTG CGAAATCTTG TGAAAAGGCT ATCAGAGGTA 
TCATCCTGGA TAACACATGT TATTGTCAGT CAGCCAACAC ATGACGATCG AAAGGTTGCT 
CTTACTGCAA TTTTGAGAAT TGTGGAAACT TGCTGGAATA TTGGAAACTT TAATGCAGCT 
GTTGAGGTTC TTATGGGTTT AAAGAGTGAA AAACTCAGAC CATTCTGGCT TTCTTTGCGA 
CAAGAGGAAA AGTCTCAGTT TGATTCGCTT TGCGAAACCT TGCTTCCTGC AAACCAAGCT 
CTCCCATCTC AAGCTTACAT CAACGCGGTC CAACGAGCAC TGAGAATGCC ACAATCTCGT 
GTGATTCCAT TTTTTGGAAT ATTTCTTCGT GATCTCTACG CAATTGTGAA TGACTTGCCG 
AATATCGTTG TGATTGGACA AGAAGGTGAA ACCCAAAAGC TGGAATTCAT GAGCGATCCC 
AATGGCGAAG ATCATTTTTC GTCGAGAATT GGAGTGGGTG GGCTGTTGAA TGCTGATAAA 
ATCAACTTGG TGGCAATTGT TTTGGACAAC TTGGAATTAT TCCATCGTCA CAGCAGGACA 
ATGATCAAAT TGTTAGAAGA GCAAGCAGTC CCACCAATTC AAATTCCTCA GAACGAGCGG 
GAGCAAAAAG AAAAGGAAGC AAAAACATAT GAACCAGTAC AAGTGGTTCG CGGCTCTTCT 
CATGGAGTGG CTCTCATCCC TCTTGATACC CTAACATTCG ATCTTGATGT TATCCAACGA 
CTCCAGCACG GAACAACAGT GATCCATTAC GAACCTGATT CGGGAAGAAG CAATTTGTGT 
CTGCTGCGGT TAGACCCTAG TTGCGGTCAA ATAAACTGGC ATAAGATTTC ATATTCAGTT 
AACAAAGATC CCAAAGAAAA GGATGTGCTC GCCAAAGTAT CCGTTTCAAA TCTGCAGCCG 
CTAGACTCTG GCCGAGGGGC TCCGTCACCA ATGCCATCTG GAAGAACACC AGGAACTGGA 
GGAGTTGGAG TAGAGGAGGG AGAACTCAAA TTAAGTGTTG TGAAAGGAGT CGAACTAGTT 
GATAGTTATG ATATTGATAT TGAGGCAATT TACCGTCGTC ATTCTATGGA AGAGATGTCA 
GTTCCTGTTA GCTGTTGGAA AGTTAGTCAT GGTCAGTTGT TATCGGATAA TGAGTTCATC 
TACTTTTTGG CCCCTCAACA AATTGCTCAA TTTTGGACCA ACGGGTTGCA ATCTGTAGTT 
AAAAGTCTCC AAGGACAGCA AAGGTACCCT GATCGGCGAA TGTTATGGAT CAAAAATGTC 
TACTTATCAC TTTACGAGAT AACGGGCGAA TCAAACTGTG GACCACGGCC ATTTGAAGCA 
CTTCAGGCAT TTGGTGGGCG GGTTGAGCGT TGGAAAGGCT TTGGGCTGAG TCAAACAAAT 
ACTAACGCAA CACGACCCAA TGACTCGTCG CTGTCCAGTG AGCCAGGAGG AGCTAAAAGC 
AGACTGAAGA ACCTTAAAAA TGCTATGCAA AAGAAGCTTC GTGGTGCATC GCGCGAAGGA 
AGCAGATCTC AGTCACCTCA ACCTCATTCT CCTCTTGTCC GACCACCATC AATAAAATCG 
CAAATATCGT CACAATCTGG TCCGCCGGGT CCGAACTCTC CAGGCTATTT GCTGAAACCA 
CGTGGAGAAC CTGCCAATAG TGATGCAGGA GACATTGATA GCATTTATAC ACCTCGATCA 
AGAACTCCAA CAAGCAGCTC CTATGGAGGG CGATCCGTTG GAGGGAGATC ATGTAAATCG 
TGGAGATCAA GAGGAGGAGA GACACCAAAT TCGGGTTCCA TAAGCAGCTC GGGTCAGATG 
TCAATTCAAG TGTCTGGTCT GAGTGGACCA TCGGGAAAAG AGTTTCAGGA AAAACCATTA 
ACACTTGTAG AGTTCGCTGA ACTTTTTCGA TTGTTCAATA CAAGAATGAG AAAAGATTTG 
AGAGATGTGT TCAATGATGT CCTGTCTACG GCAACTACTC CTCAGCATTG TCCTAAAAGA 
GAACGTGATC GCCACAGTCC GAGAATGCAG TCAAGGCTGG CGAGTGTCTC CAACTCGTAC 
AATGCAGATT TTCTGTCTAA CGATTTCTTG ACTCGGAATA CGGCAGTAAC GTCCCATCAT 
ATCAGTGAAA AGCAAAACAA AATCTACAAT GCATTGGCAC TTGCGAGTGT TAATAGCATG 
GGCGGACTCA TGGACACTTC CCGGTCGTCG ATGCTGACGC CGCAAATGCT GCGCGCTTTT 
GTCAACACTC ATCAGATGGA GCAGATTGAC GAGCAAACGG CGATTAAGCT TATTCAGGAT 
CATGAACCTG ATGGTATCTG TCGACAAAAG AATCAAATGT CATTCGAAGG ATTCACTAGA 
TTCCTTTGCG ATCCTGTCAA CTTTGCATTC GTACCGGAAA CAATTGAACC AGATGAAGAG 
GACCTACGGT ACCCGTTGTC TCATTATTAC ATCAATTCAA GTCACAACAC CTATTTGACG 
GGACATCAAC TCAAGGGACC CAGTAGCTCG GAGATGTACA GACAGGTTCT CCTCACCGGG 
TGTCGGTGTG TCGAGCTTGA TTGTTGGGAT GGAGATGACG GATTGCCATT GATTTATCAT 
GGACACACAT TGGTTAGCAA GATTGGGTTT AGACAGGTGG TCGAAATTAT CAAAAAATCT 
GCATTCATAA CATCGGATCT TCCTGTGATA TTATCAATTG AGAATCATTG TTCCCTACAA 
CAACAGGCGA AAATGGCACA GATGTTCAAA ACAGTTCTTG GAGATTTACT AGTATCAAAC 
TTCCTTTTTG AAGCTGATTT CTCGGACTCT CCACGTCTTC CATGCCCTCT CCAAATGAAA 
AATAAGATCT TGATAAAAAA TAAGAAAATG ATAGTCGACC CACCAACTCC ATTGCCAATG 
ATTGAACGAG GAGCTGTGCA GCGTGGTGAA ACTCAATTAA ATCTTCACAG AAAACAGAGT 
AAAAACTCCT ACGAAAGCAG TACGGTAGAT GAAGTGGAAG ATGACGACCT AGATGAGTTT 
CTTGACGATG AAGAAAATGA GGAGGATGAC CAAGAAGAGG TTCAAGTCAG AAGTGAAAAA 
GAAGACAGTC CGAAGACATC AAAACGTGCA GAGAAATCGG CTAGAAACAT CAAACAGCAA 
GATTCGTTAT GTAGCGATCA TTCGGTAGAG CAGGCAAAAC CTTCCACAAG CAAGACCACA 
TCCAAAACCA ACGATAGAAA GACTGAAGAT GAAGTACTTT ACGCTCAACT TGCTCAAAAT 
GCCATCCGAA ACCAACAACC AAGAAAGAAC AATACTGGAG TCCAAATAGC GCCAGAGTTG 
TCAGATATTG TCATTTACAT GCAAGCGACA AAATTCAAAG GATTTCCCCC AGTCGATGGC 
ATTCAATCAC CAAGGATAAT GGAGGAAGGG CCAGCTTCTG CAAGTCTTTC ATTTTCATCA 
CGGGCTCGTA CGCCATCAAA TCTGCTGAAT ACACCAGCCC CTCCACGACG ACAGCGAAGT 
AGCACTCAGT TGAGTCAAGA ACTGGCTGCA GAATTTTTGG GATCAGTTAG GGCAAATGCT 
ACAGCTACAT GCTACCAGGT TACATCTCTT AATGAAAACG CTGCAAAGAA ACTGATGAAA 
AGACATCCAG CAAAATGCGT ATCTTATACT CGGGACCATC TGATTCGTAC GTATCCAAGT 
GCCAAGCACT ATGACTCGTC CAACTTTAAC CCCATCAACT GCTGGGCTCA TGGAATGCAA 
ATGGTTGCTT TAAATTTTCA AACTCCTGAT GTTATAATGG CAGTTAACCA AGCGATGTTC 
GAGCAATCTG GAAACTGTGG ATATCAACTG AAACCACGTT GTCTTTGGGA TGAATCTCAT 
CTTTTGTACA ACAAATTTTT GCCTCTCTCC AAAGACATTG CCGGCCATTC TGCTTTGTTA 
CTAAACTTAA CTATTATATC CGGACAGCAC GTGTATCCAA ACACCCATTA CGCCAGTTTA 
TATGTCGAAA TTGAAGTCAT CGGTATTCAC AACGACTGTG TTCGTGAGAA ATCAAAAGTG 
GTCCAGAGAA ATTCTGTGAA TCCAATCTGG AACCACACCA CTCAACTTCG AATAGCTTGT 
GTTGACTTAG CATTCCTTCG CATAGCAGTA TGTGATAGTG GGCAAAATGG AAGAGTCGTT 
GCTCACCGAG TTGTTCCTGT CAAATGCATT CGTCCGGGTT TCCGACATCT CCCACTCAGA 
ACTCCAACTA ATCTTCCGAT CGACAACGCT ATGATATTTT TAAGAACAAG ATTCGAGCAA 
GAAGAACACA TCTACCTCCA CGACGATGAT TCAAACACCT ATTGTAATCT AGAACATACG 
CTGGCATACC GCACAGACTT AACACCCAAT TTATCTCCAA CGCCAATTTT GAAGAAACAG 
ATCTTTGTTC TTCGAATTAC AGGCGCGTTT GCTGATGAAA CAGCAATTAC AGTGCACAGC 
GAGTCAGGAA GTACAGTGAA GACGGTGATG CAACAGGCGC TGCTTAACGC TGGAAAGAAT 
GCGGATCAGG TTGAAGAGTA TGTACTCATC GAAGAATCAC TGCCGGCACC TTCTGGGGAA 
GATCCAATTG AGCAGAGAGT ACTTCCTCTG AACGAGCCTA TCATGGACGC CGTGGCCTGC 
TGGAACGGTT CAATGCGTCG TTTTGTGTTA CGAAAAAAAG GCAGTGACCC ATCATCTAGA 
GCTTGGATCA CTTCAATTAT AAAATCCGGA ACAAGTGGAA GCTCCACGTC AGTCTCACCA 
AGTCCGTTGA CCAAAGATGG ACACGTAAAG AGTGCATCAA GCAATCAACT TCATGGACGA 
TCATTGGACA CTGACGCTTT TGGAGAGCAC CTTGAAGTGA CAGAAGGGTT GAACCCTCGT 
GCAAGATCAA TGGGCGACAC ATTTCTGGTA TGTGTTCACA ATGTTAGTGA AGATCAGCCT 
TATGCAATCC TACGTGCTGG AATCCACAGC ACTGCCGCCG ACATTATTCG TCAGGTGTTT 
GTTAAAGCTC GCCGTTCAAA TGTAGACGAT TCCGAGTTTG TTCTTGTTGA AGAAACCTGC 
GATGATCCAA AACTCAATCA AGGCCAAATG ACTCCAAAGT ATCCAAACAA CCGCACCACA 
AGCCGAGTGT TAGGACAAAA CGAAAATGTT TGGAAAGCAC AATCGAGGTG GAAGTCAATG 
GGCAGATTTG TACTTGAAAA CCGAAAGGAC ACAGTGCACG CAACCCTCGA AAAAGTGAGA 
TCATTTATAT CAAAACTGGA AGCTGCAAAA GCAAGCATGG ATCCCAGACA CGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_001136455.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001129927.1 Caenorhabditis elegans Phosphoinositide phospholipase C (plc-1), mRNA.

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CloneID	OCa349047
Clone ID Related Accession (Same CDS sequence)	NM_001136455.3 , NM_001136455.2
Accession Version	NM_001136455.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Phosphoinositide phospholipase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_001136455.2.

ATGCCTCACG ATAGTAAAAT GACATCGGTT GTGTCCCGTT TGCTTCATGC TCTCGGAAAA 
TCTCATGATC ATGAAGAACC TGAAACTCCG CCTAATCCCG ATAGTCTTCT TGTATATGTG 
TGTAAATTGG CAACATCACG TAGTGACTCA ACCTCTTCAA CGCAATCAGC CACAGCTGGA 
TTCATTCTCA ATGAAGAACC AATCACACTG TTCCGACTTG AACTCGAACG ACTTCAATAT 
ATTCTTCATT TCCCTGAAGA AGTTGCATTC CAACTATCTT CTACTGAGTA TCAGCTTTTC 
TACTCAATAC AACCCATGGA TTATGTTCGT TACGTCAGTT GCGATCTCAC ATCTGTTCCA 
GTGTCAGAAA ATCCAAGTCC AGTGCGAAAT CTTGTGAAAA GGCTATCAGA GGTATCATCC 
TGGATAACAC ATGTTATTGT CAGTCAGCCA ACACATGACG ATCGAAAGGT TGCTCTTACT 
GCAATTTTGA GAATTGTGGA AACTTGCTGG AATATTGGAA ACTTTAATGC AGCTGTTGAG 
GTTCTTATGG GTTTAAAGAG TGAAAAACTC AGACCATTCT GGCTTTCTTT GCGACAAGAG 
GAAAAGTCTC AGTTTGATTC GCTTTGCGAA ACCTTGCTTC CTGCAAACCA AGCTCTCCCA 
TCTCAAGCTT ACATCAACGC GGTCCAACGA GCACTGAGAA TGCCACAATC TCGTGTGATT 
CCATTTTTTG GAATATTTCT TCGTGATCTC TACGCAATTG TGAATGACTT GCCGAATATC 
GTTGTGATTG GACAAGAAGG TGAAACCCAA AAGCTGGAAT TCATGAGCGA TCCCAATGGC 
GAAGATCATT TTTCGTCGAG AATTGGAGTG GGTGGGCTGT TGAATGCTGA TAAAATCAAC 
TTGGTGGCAA TTGTTTTGGA CAACTTGGAA TTATTCCATC GTCACAGCAG GACAATGATC 
AAATTGTTAG AAGAGCAAGC AGTCCCACCA ATTCAAATTC CTCAGAACGA GCGGGAGCAA 
AAAGAAAAGG AAGCAAAAAC ATATGAACCA GTACAAGTGG TTCGCGGCTC TTCTCATGGA 
GTGGCTCTCA TCCCTCTTGA TACCCTAACA TTCGATCTTG ATGTTATCCA ACGACTCCAG 
CACGGAACAA CAGTGATCCA TTACGAACCT GATTCGGGAA GAAGCAATTT GTGTCTGCTG 
CGGTTAGACC CTAGTTGCGG TCAAATAAAC TGGCATAAGA TTTCATATTC AGTTAACAAA 
GATCCCAAAG AAAAGGATGT GCTCGCCAAA GTATCCGTTT CAAATCTGCA GCCGCTAGAC 
TCTGGCCGAG GGGCTCCGTC ACCAATGCCA TCTGGAAGAA CACCAGGAAC TGGAGGAGTT 
GGAGTAGAGG AGGGAGAACT CAAATTAAGT GTTGTGAAAG GAGTCGAACT AGTTGATAGT 
TATGATATTG ATATTGAGGC AATTTACCGT CGTCATTCTA TGGAAGAGAT GTCAGTTCCT 
GTTAGCTGTT GGAAAGTTAG TCATGGTCAG TTGTTATCGG ATAATGAGTT CATCTACTTT 
TTGGCCCCTC AACAAATTGC TCAATTTTGG ACCAACGGGT TGCAATCTGT AGTTAAAAGT 
CTCCAAGGAC AGCAAAGGTA CCCTGATCGG CGAATGTTAT GGATCAAAAA TGTCTACTTA 
TCACTTTACG AGATAACGGG CGAATCAAAC TGTGGACCAC GGCCATTTGA AGCACTTCAG 
GCATTTGGGC TGAGTCAAAC AAATACTAAC GCAACACGAC CCAATGACTC GTCGCTGTCC 
AGTGAGCCAG GAGGAGCTAA AAGCAGACTG AAGAACCTTA AAAATGCTAT GCAAAAGAAG 
CTTCGTGGTG CATCGCGCGA AGGAAGCAGA TCTCAGTCAC CTCAACCTCA TTCTCCTCTT 
GTCCGACCAC CATCAATAAA ATCGCAAATA TCGTCACAAT CTGGTCCGCC GGGTCCGAAC 
TCTCCAGGCT ATTTGCTGAA ACCACGTGGA GAACCTGCCA ATAGTGATGC AGGAGACATT 
GATAGCATTT ATACACCTCG ATCAAGAACT CCAACAAGCA GCTCCTATGG AGGGCGATCC 
GTTGGAGGGA GATCATGTAA ATCGTGGAGA TCAAGAGGAG GAGAGACACC AAATTCGGGT 
TCCATAAGCA GCTCGGGTCA GATGTCAATT CAAGTGTCTG GTCTGAGTGG ACCATCGGGA 
AAAGAGTTTC AGGAAAAACC ATTAACACTT GTAGAGTTCG CTGAACTTTT TCGATTGTTC 
AATACAAGAA TGAGAAAAGA TTTGAGAGAT GTGTTCAATG ATGTCCTGTC TACGGCAACT 
ACTCCTCAGC ATTGTCCTAA AAGAGAACGT GATCGCCACA GTCCGAGAAT GCAGTCAAGG 
CTGGCGAGTG TCTCCAACTC GTACAATGCA GATTTTCTGT CTAACGATTT CTTGACTCGG 
AATACGGCAG TAACGTCCCA TCATATCAGT GAAAAGCAAA ACAAAATCTA CAATGCATTG 
GCACTTGCGA GTGTTAATAG CATGGGCGGA CTCATGGACA CTTCCCGGTC GTCGATGCTG 
ACGCCGCAAA TGCTGCGCGC TTTTGTCAAC ACTCATCAGA TGGAGCAGAT TGACGAGCAA 
ACGGCGATTA AGCTTATTCA GGATCATGAA CCTGATGGTA TCTGTCGACA AAAGAATCAA 
ATGTCATTCG AAGGATTCAC TAGATTCCTT TGCGATCCTG TCAACTTTGC ATTCGTACCG 
GAAACAATTG AACCAGATGA AGAGGACCTA CGGTACCCGT TGTCTCATTA TTACATCAAT 
TCAAGTCACA ACACCTATTT GACGGGACAT CAACTCAAGG GACCCAGTAG CTCGGAGATG 
TACAGACAGG TTCTCCTCAC CGGGTGTCGG TGTGTCGAGC TTGATTGTTG GGATGGAGAT 
GACGGATTGC CATTGATTTA TCATGGACAC ACATTGGTTA GCAAGATTGG GTTTAGACAG 
GTGGTCGAAA TTATCAAAAA ATCTGCATTC ATAACATCGG ATCTTCCTGT GATATTATCA 
ATTGAGAATC ATTGTTCCCT ACAACAACAG GCGAAAATGG CACAGATGTT CAAAACAGTT 
CTTGGAGATT TACTAGTATC AAACTTCCTT TTTGAAGCTG ATTTCTCGGA CTCTCCACGT 
CTTCCATGCC CTCTCCAAAT GAAAAATAAG ATCTTGATAA AAAATAAGAA AATGATAGTC 
GACCCACCAA CTCCATTGCC AATGATTGAA CGAGGAGCTG TGCAGCGTGG TGAAACTCAA 
TTAAATCTTC ACAGAAAACA GAGTAAAAAC TCCTACGAAA GCAGTACGGT AGATGAAGTG 
GAAGATGACG ACCTAGATGA GTTTCTTGAC GATGAAGAAA ATGAGGAGGA TGACCAAGAA 
GAGGTTCAAG TCAGAAGTGA AAAAGAAGAC AGTCCGAAGA CATCAAAACG TGCAGAGAAA 
TCGGCTAGAA ACATCAAACA GCAAGATTCG TTATGTAGCG ATCATTCGGT AGAGCAGGCA 
AAACCTTCCA CAAGCAAGAC CACATCCAAA ACCAACGATA GAAAGACTGA AGATGAAGTA 
CTTTACGCTC AACTTGCTCA AAATGCCATC CGAAACCAAC AACCAAGAAA GAACAATACT 
GGAGTCCAAA TAGCGCCAGA GTTGTCAGAT ATTGTCATTT ACATGCAAGC GACAAAATTC 
AAAGGATTTC CCCCAGTCGA TGGCATTCAA TCACCAAGGA TAATGGAGGA AGGGCCAGCT 
TCTGCAAGTC TTTCATTTTC ATCACGGGCT CGTACGCCAT CAAATCTGCT GAATACACCA 
GCCCCTCCAC GACGACAGCG AAGTAGCACT CAGTTGAGTC AAGAACTGGC TGCAGAATTT 
TTGGGATCAG TTAGGGCAAA TGCTACAGCT ACATGCTACC AGGTTACATC TCTTAATGAA 
AACGCTGCAA AGAAACTGAT GAAAAGACAT CCAGCAAAAT GCGTATCTTA TACTCGGGAC 
CATCTGATTC GTACGTATCC AAGTGCCAAG CACTATGACT CGTCCAACTT TAACCCCATC 
AACTGCTGGG CTCATGGAAT GCAAATGGTT GCTTTAAATT TTCAAACTCC TGATGTTATA 
ATGGCAGTTA ACCAAGCGAT GTTCGAGCAA TCTGGAAACT GTGGATATCA ACTGAAACCA 
CGTTGTCTTT GGGATGAATC TCATCTTTTG TACAACAAAT TTTTGCCTCT CTCCAAAGAC 
ATTGCCGGCC ATTCTGCTTT GTTACTAAAC TTAACTATTA TATCCGGACA GCACGTGTAT 
CCAAACACCC ATTACGCCAG TTTATATGTC GAAATTGAAG TCATCGGTAT TCACAACGAC 
TGTGTTCGTG AGAAATCAAA AGTGGTCCAG AGAAATTCTG TGAATCCAAT CTGGAACCAC 
ACCACTCAAC TTCGAATAGC TTGTGTTGAC TTAGCATTCC TTCGCATAGC AGTATGTGAT 
AGTGGGCAAA ATGGAAGAGT CGTTGCTCAC CGAGTTGTTC CTGTCAAATG CATTCGTCCG 
GGTTTCCGAC ATCTCCCACT CAGAACTCCA ACTAATCTTC CGATCGACAA CGCTATGATA 
TTTTTAAGAA CAAGATTCGA GCAAGAAGAA CACATCTACC TCCACGACGA TGATTCAAAC 
ACCTATTGTA ATCTAGAACA TACGCTGGCA TACCGCACAG ACTTAACACC CAATTTATCT 
CCAACGCCAA TTTTGAAGAA ACAGATCTTT GTTCTTCGAA TTACAGGCGC GTTTGCTGAT 
GAAACAGCAA TTACAGTGCA CAGCGAGTCA GGAAGTACAG TGAAGACGGT GATGCAACAG 
GCGCTGCTTA ACGCTGGAAA GAATGCGGAT CAGGTTGAAG AGTATGTACT CATCGAAGAA 
TCACTGCCGG CACCTTCTGG GGAAGATCCA ATTGAGCAGA GAGTACTTCC TCTGAACGAG 
CCTATCATGG ACGCCGTGGC CTGCTGGAAC GGTTCAATGC GTCGTTTTGT GTTACGAAAA 
AAAGGCAGTG ACCCATCATC TAGAGCTTGG ATCACTTCAA TTATAAAATC CGGAACAAGT 
GGAAGCTCCA CGTCAGTCTC ACCAAGTCCG TTGACCAAAG ATGGACACGT AAAGAGTGCA 
TCAAGCAATC AACTTCATGG ACGATCATTG GACACTGACG CTTTTGGAGA GCACCTTGAA 
GTGACAGAAG GGTTGAACCC TCGTGCAAGA TCAATGGGCG ACACATTTCT GGTATGTGTT 
CACAATGTTA GTGAAGATCA GCCTTATGCA ATCCTACGTG CTGGAATCCA CAGCACTGCC 
GCCGACATTA TTCGTCAGGT GTTTGTTAAA GCTCGCCGTT CAAATGTAGA CGATTCCGAG 
TTTGTTCTTG TTGAAGAAAC CTGCGATGAT CCAAAACTCA ATCAAGGCCA AATGACTCCA 
AAGTATCCAA ACAACCGCAC CACAAGCCGA GTGTTAGGAC AAAACGAAAA TGTTTGGAAA 
GCACAATCGA GGTGGAAGTC AATGGGCAGA TTTGTACTTG AAAACCGAAA GGACACAGTG 
CACGCAACCC TCGAAAAAGT GAGATCATTT ATATCAAAAC TGGAAGCTGC AAAAGCAAGC 
ATGGATCCCA GACACGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129927.1
CDS	169..5829
Translation	MPHDSKMTSVVSRLLHALGKSHDHEEPETPPNPDSLLVYVCKLATSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKVRSFISKLEAAKASMDPRHE

Target ORF information:

RefSeq Version	NM_001136455.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Phosphoinositide phospholipase C (plc-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136455.3

ORF Insert Sequence:

ATGCCTCACG ATAGTAAAAT GACATCGGTT GTGTCCCGTT TGCTTCATGC TCTCGGAAAA 
TCTCATGATC ATGAAGAACC TGAAACTCCG CCTAATCCCG ATAGTCTTCT TGTATATGTG 
TGTAAATTGG CAACATCACG TAGTGACTCA ACCTCTTCAA CGCAATCAGC CACAGCTGGA 
TTCATTCTCA ATGAAGAACC AATCACACTG TTCCGACTTG AACTCGAACG ACTTCAATAT 
ATTCTTCATT TCCCTGAAGA AGTTGCATTC CAACTATCTT CTACTGAGTA TCAGCTTTTC 
TACTCAATAC AACCCATGGA TTATGTTCGT TACGTCAGTT GCGATCTCAC ATCTGTTCCA 
GTGTCAGAAA ATCCAAGTCC AGTGCGAAAT CTTGTGAAAA GGCTATCAGA GGTATCATCC 
TGGATAACAC ATGTTATTGT CAGTCAGCCA ACACATGACG ATCGAAAGGT TGCTCTTACT 
GCAATTTTGA GAATTGTGGA AACTTGCTGG AATATTGGAA ACTTTAATGC AGCTGTTGAG 
GTTCTTATGG GTTTAAAGAG TGAAAAACTC AGACCATTCT GGCTTTCTTT GCGACAAGAG 
GAAAAGTCTC AGTTTGATTC GCTTTGCGAA ACCTTGCTTC CTGCAAACCA AGCTCTCCCA 
TCTCAAGCTT ACATCAACGC GGTCCAACGA GCACTGAGAA TGCCACAATC TCGTGTGATT 
CCATTTTTTG GAATATTTCT TCGTGATCTC TACGCAATTG TGAATGACTT GCCGAATATC 
GTTGTGATTG GACAAGAAGG TGAAACCCAA AAGCTGGAAT TCATGAGCGA TCCCAATGGC 
GAAGATCATT TTTCGTCGAG AATTGGAGTG GGTGGGCTGT TGAATGCTGA TAAAATCAAC 
TTGGTGGCAA TTGTTTTGGA CAACTTGGAA TTATTCCATC GTCACAGCAG GACAATGATC 
AAATTGTTAG AAGAGCAAGC AGTCCCACCA ATTCAAATTC CTCAGAACGA GCGGGAGCAA 
AAAGAAAAGG AAGCAAAAAC ATATGAACCA GTACAAGTGG TTCGCGGCTC TTCTCATGGA 
GTGGCTCTCA TCCCTCTTGA TACCCTAACA TTCGATCTTG ATGTTATCCA ACGACTCCAG 
CACGGAACAA CAGTGATCCA TTACGAACCT GATTCGGGAA GAAGCAATTT GTGTCTGCTG 
CGGTTAGACC CTAGTTGCGG TCAAATAAAC TGGCATAAGA TTTCATATTC AGTTAACAAA 
GATCCCAAAG AAAAGGATGT GCTCGCCAAA GTATCCGTTT CAAATCTGCA GCCGCTAGAC 
TCTGGCCGAG GGGCTCCGTC ACCAATGCCA TCTGGAAGAA CACCAGGAAC TGGAGGAGTT 
GGAGTAGAGG AGGGAGAACT CAAATTAAGT GTTGTGAAAG GAGTCGAACT AGTTGATAGT 
TATGATATTG ATATTGAGGC AATTTACCGT CGTCATTCTA TGGAAGAGAT GTCAGTTCCT 
GTTAGCTGTT GGAAAGTTAG TCATGGTCAG TTGTTATCGG ATAATGAGTT CATCTACTTT 
TTGGCCCCTC AACAAATTGC TCAATTTTGG ACCAACGGGT TGCAATCTGT AGTTAAAAGT 
CTCCAAGGAC AGCAAAGGTA CCCTGATCGG CGAATGTTAT GGATCAAAAA TGTCTACTTA 
TCACTTTACG AGATAACGGG CGAATCAAAC TGTGGACCAC GGCCATTTGA AGCACTTCAG 
GCATTTGGGC TGAGTCAAAC AAATACTAAC GCAACACGAC CCAATGACTC GTCGCTGTCC 
AGTGAGCCAG GAGGAGCTAA AAGCAGACTG AAGAACCTTA AAAATGCTAT GCAAAAGAAG 
CTTCGTGGTG CATCGCGCGA AGGAAGCAGA TCTCAGTCAC CTCAACCTCA TTCTCCTCTT 
GTCCGACCAC CATCAATAAA ATCGCAAATA TCGTCACAAT CTGGTCCGCC GGGTCCGAAC 
TCTCCAGGCT ATTTGCTGAA ACCACGTGGA GAACCTGCCA ATAGTGATGC AGGAGACATT 
GATAGCATTT ATACACCTCG ATCAAGAACT CCAACAAGCA GCTCCTATGG AGGGCGATCC 
GTTGGAGGGA GATCATGTAA ATCGTGGAGA TCAAGAGGAG GAGAGACACC AAATTCGGGT 
TCCATAAGCA GCTCGGGTCA GATGTCAATT CAAGTGTCTG GTCTGAGTGG ACCATCGGGA 
AAAGAGTTTC AGGAAAAACC ATTAACACTT GTAGAGTTCG CTGAACTTTT TCGATTGTTC 
AATACAAGAA TGAGAAAAGA TTTGAGAGAT GTGTTCAATG ATGTCCTGTC TACGGCAACT 
ACTCCTCAGC ATTGTCCTAA AAGAGAACGT GATCGCCACA GTCCGAGAAT GCAGTCAAGG 
CTGGCGAGTG TCTCCAACTC GTACAATGCA GATTTTCTGT CTAACGATTT CTTGACTCGG 
AATACGGCAG TAACGTCCCA TCATATCAGT GAAAAGCAAA ACAAAATCTA CAATGCATTG 
GCACTTGCGA GTGTTAATAG CATGGGCGGA CTCATGGACA CTTCCCGGTC GTCGATGCTG 
ACGCCGCAAA TGCTGCGCGC TTTTGTCAAC ACTCATCAGA TGGAGCAGAT TGACGAGCAA 
ACGGCGATTA AGCTTATTCA GGATCATGAA CCTGATGGTA TCTGTCGACA AAAGAATCAA 
ATGTCATTCG AAGGATTCAC TAGATTCCTT TGCGATCCTG TCAACTTTGC ATTCGTACCG 
GAAACAATTG AACCAGATGA AGAGGACCTA CGGTACCCGT TGTCTCATTA TTACATCAAT 
TCAAGTCACA ACACCTATTT GACGGGACAT CAACTCAAGG GACCCAGTAG CTCGGAGATG 
TACAGACAGG TTCTCCTCAC CGGGTGTCGG TGTGTCGAGC TTGATTGTTG GGATGGAGAT 
GACGGATTGC CATTGATTTA TCATGGACAC ACATTGGTTA GCAAGATTGG GTTTAGACAG 
GTGGTCGAAA TTATCAAAAA ATCTGCATTC ATAACATCGG ATCTTCCTGT GATATTATCA 
ATTGAGAATC ATTGTTCCCT ACAACAACAG GCGAAAATGG CACAGATGTT CAAAACAGTT 
CTTGGAGATT TACTAGTATC AAACTTCCTT TTTGAAGCTG ATTTCTCGGA CTCTCCACGT 
CTTCCATGCC CTCTCCAAAT GAAAAATAAG ATCTTGATAA AAAATAAGAA AATGATAGTC 
GACCCACCAA CTCCATTGCC AATGATTGAA CGAGGAGCTG TGCAGCGTGG TGAAACTCAA 
TTAAATCTTC ACAGAAAACA GAGTAAAAAC TCCTACGAAA GCAGTACGGT AGATGAAGTG 
GAAGATGACG ACCTAGATGA GTTTCTTGAC GATGAAGAAA ATGAGGAGGA TGACCAAGAA 
GAGGTTCAAG TCAGAAGTGA AAAAGAAGAC AGTCCGAAGA CATCAAAACG TGCAGAGAAA 
TCGGCTAGAA ACATCAAACA GCAAGATTCG TTATGTAGCG ATCATTCGGT AGAGCAGGCA 
AAACCTTCCA CAAGCAAGAC CACATCCAAA ACCAACGATA GAAAGACTGA AGATGAAGTA 
CTTTACGCTC AACTTGCTCA AAATGCCATC CGAAACCAAC AACCAAGAAA GAACAATACT 
GGAGTCCAAA TAGCGCCAGA GTTGTCAGAT ATTGTCATTT ACATGCAAGC GACAAAATTC 
AAAGGATTTC CCCCAGTCGA TGGCATTCAA TCACCAAGGA TAATGGAGGA AGGGCCAGCT 
TCTGCAAGTC TTTCATTTTC ATCACGGGCT CGTACGCCAT CAAATCTGCT GAATACACCA 
GCCCCTCCAC GACGACAGCG AAGTAGCACT CAGTTGAGTC AAGAACTGGC TGCAGAATTT 
TTGGGATCAG TTAGGGCAAA TGCTACAGCT ACATGCTACC AGGTTACATC TCTTAATGAA 
AACGCTGCAA AGAAACTGAT GAAAAGACAT CCAGCAAAAT GCGTATCTTA TACTCGGGAC 
CATCTGATTC GTACGTATCC AAGTGCCAAG CACTATGACT CGTCCAACTT TAACCCCATC 
AACTGCTGGG CTCATGGAAT GCAAATGGTT GCTTTAAATT TTCAAACTCC TGATGTTATA 
ATGGCAGTTA ACCAAGCGAT GTTCGAGCAA TCTGGAAACT GTGGATATCA ACTGAAACCA 
CGTTGTCTTT GGGATGAATC TCATCTTTTG TACAACAAAT TTTTGCCTCT CTCCAAAGAC 
ATTGCCGGCC ATTCTGCTTT GTTACTAAAC TTAACTATTA TATCCGGACA GCACGTGTAT 
CCAAACACCC ATTACGCCAG TTTATATGTC GAAATTGAAG TCATCGGTAT TCACAACGAC 
TGTGTTCGTG AGAAATCAAA AGTGGTCCAG AGAAATTCTG TGAATCCAAT CTGGAACCAC 
ACCACTCAAC TTCGAATAGC TTGTGTTGAC TTAGCATTCC TTCGCATAGC AGTATGTGAT 
AGTGGGCAAA ATGGAAGAGT CGTTGCTCAC CGAGTTGTTC CTGTCAAATG CATTCGTCCG 
GGTTTCCGAC ATCTCCCACT CAGAACTCCA ACTAATCTTC CGATCGACAA CGCTATGATA 
TTTTTAAGAA CAAGATTCGA GCAAGAAGAA CACATCTACC TCCACGACGA TGATTCAAAC 
ACCTATTGTA ATCTAGAACA TACGCTGGCA TACCGCACAG ACTTAACACC CAATTTATCT 
CCAACGCCAA TTTTGAAGAA ACAGATCTTT GTTCTTCGAA TTACAGGCGC GTTTGCTGAT 
GAAACAGCAA TTACAGTGCA CAGCGAGTCA GGAAGTACAG TGAAGACGGT GATGCAACAG 
GCGCTGCTTA ACGCTGGAAA GAATGCGGAT CAGGTTGAAG AGTATGTACT CATCGAAGAA 
TCACTGCCGG CACCTTCTGG GGAAGATCCA ATTGAGCAGA GAGTACTTCC TCTGAACGAG 
CCTATCATGG ACGCCGTGGC CTGCTGGAAC GGTTCAATGC GTCGTTTTGT GTTACGAAAA 
AAAGGCAGTG ACCCATCATC TAGAGCTTGG ATCACTTCAA TTATAAAATC CGGAACAAGT 
GGAAGCTCCA CGTCAGTCTC ACCAAGTCCG TTGACCAAAG ATGGACACGT AAAGAGTGCA 
TCAAGCAATC AACTTCATGG ACGATCATTG GACACTGACG CTTTTGGAGA GCACCTTGAA 
GTGACAGAAG GGTTGAACCC TCGTGCAAGA TCAATGGGCG ACACATTTCT GGTATGTGTT 
CACAATGTTA GTGAAGATCA GCCTTATGCA ATCCTACGTG CTGGAATCCA CAGCACTGCC 
GCCGACATTA TTCGTCAGGT GTTTGTTAAA GCTCGCCGTT CAAATGTAGA CGATTCCGAG 
TTTGTTCTTG TTGAAGAAAC CTGCGATGAT CCAAAACTCA ATCAAGGCCA AATGACTCCA 
AAGTATCCAA ACAACCGCAC CACAAGCCGA GTGTTAGGAC AAAACGAAAA TGTTTGGAAA 
GCACAATCGA GGTGGAAGTC AATGGGCAGA TTTGTACTTG AAAACCGAAA GGACACAGTG 
CACGCAACCC TCGAAAAAGT GAGATCATTT ATATCAAAAC TGGAAGCTGC AAAAGCAAGC 
ATGGATCCCA GACACGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_001136455.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001129927.1 Caenorhabditis elegans Phosphoinositide phospholipase C (plc-1), partial mRNA.

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CloneID	OCa349047
Clone ID Related Accession (Same CDS sequence)	NM_001136455.3 , NM_001136455.2
Accession Version	NM_001136455.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Phosphoinositide phospholipase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_001136455.1. COMPLETENESS: incomplete on both ends.

ATGCCTCACG ATAGTAAAAT GACATCGGTT GTGTCCCGTT TGCTTCATGC TCTCGGAAAA 
TCTCATGATC ATGAAGAACC TGAAACTCCG CCTAATCCCG ATAGTCTTCT TGTATATGTG 
TGTAAATTGG CAACATCACG TAGTGACTCA ACCTCTTCAA CGCAATCAGC CACAGCTGGA 
TTCATTCTCA ATGAAGAACC AATCACACTG TTCCGACTTG AACTCGAACG ACTTCAATAT 
ATTCTTCATT TCCCTGAAGA AGTTGCATTC CAACTATCTT CTACTGAGTA TCAGCTTTTC 
TACTCAATAC AACCCATGGA TTATGTTCGT TACGTCAGTT GCGATCTCAC ATCTGTTCCA 
GTGTCAGAAA ATCCAAGTCC AGTGCGAAAT CTTGTGAAAA GGCTATCAGA GGTATCATCC 
TGGATAACAC ATGTTATTGT CAGTCAGCCA ACACATGACG ATCGAAAGGT TGCTCTTACT 
GCAATTTTGA GAATTGTGGA AACTTGCTGG AATATTGGAA ACTTTAATGC AGCTGTTGAG 
GTTCTTATGG GTTTAAAGAG TGAAAAACTC AGACCATTCT GGCTTTCTTT GCGACAAGAG 
GAAAAGTCTC AGTTTGATTC GCTTTGCGAA ACCTTGCTTC CTGCAAACCA AGCTCTCCCA 
TCTCAAGCTT ACATCAACGC GGTCCAACGA GCACTGAGAA TGCCACAATC TCGTGTGATT 
CCATTTTTTG GAATATTTCT TCGTGATCTC TACGCAATTG TGAATGACTT GCCGAATATC 
GTTGTGATTG GACAAGAAGG TGAAACCCAA AAGCTGGAAT TCATGAGCGA TCCCAATGGC 
GAAGATCATT TTTCGTCGAG AATTGGAGTG GGTGGGCTGT TGAATGCTGA TAAAATCAAC 
TTGGTGGCAA TTGTTTTGGA CAACTTGGAA TTATTCCATC GTCACAGCAG GACAATGATC 
AAATTGTTAG AAGAGCAAGC AGTCCCACCA ATTCAAATTC CTCAGAACGA GCGGGAGCAA 
AAAGAAAAGG AAGCAAAAAC ATATGAACCA GTACAAGTGG TTCGCGGCTC TTCTCATGGA 
GTGGCTCTCA TCCCTCTTGA TACCCTAACA TTCGATCTTG ATGTTATCCA ACGACTCCAG 
CACGGAACAA CAGTGATCCA TTACGAACCT GATTCGGGAA GAAGCAATTT GTGTCTGCTG 
CGGTTAGACC CTAGTTGCGG TCAAATAAAC TGGCATAAGA TTTCATATTC AGTTAACAAA 
GATCCCAAAG AAAAGGATGT GCTCGCCAAA GTATCCGTTT CAAATCTGCA GCCGCTAGAC 
TCTGGCCGAG GGGCTCCGTC ACCAATGCCA TCTGGAAGAA CACCAGGAAC TGGAGGAGTT 
GGAGTAGAGG AGGGAGAACT CAAATTAAGT GTTGTGAAAG GAGTCGAACT AGTTGATAGT 
TATGATATTG ATATTGAGGC AATTTACCGT CGTCATTCTA TGGAAGAGAT GTCAGTTCCT 
GTTAGCTGTT GGAAAGTTAG TCATGGTCAG TTGTTATCGG ATAATGAGTT CATCTACTTT 
TTGGCCCCTC AACAAATTGC TCAATTTTGG ACCAACGGGT TGCAATCTGT AGTTAAAAGT 
CTCCAAGGAC AGCAAAGGTA CCCTGATCGG CGAATGTTAT GGATCAAAAA TGTCTACTTA 
TCACTTTACG AGATAACGGG CGAATCAAAC TGTGGACCAC GGCCATTTGA AGCACTTCAG 
GCATTTGGGC TGAGTCAAAC AAATACTAAC GCAACACGAC CCAATGACTC GTCGCTGTCC 
AGTGAGCCAG GAGGAGCTAA AAGCAGACTG AAGAACCTTA AAAATGCTAT GCAAAAGAAG 
CTTCGTGGTG CATCGCGCGA AGGAAGCAGA TCTCAGTCAC CTCAACCTCA TTCTCCTCTT 
GTCCGACCAC CATCAATAAA ATCGCAAATA TCGTCACAAT CTGGTCCGCC GGGTCCGAAC 
TCTCCAGGCT ATTTGCTGAA ACCACGTGGA GAACCTGCCA ATAGTGATGC AGGAGACATT 
GATAGCATTT ATACACCTCG ATCAAGAACT CCAACAAGCA GCTCCTATGG AGGGCGATCC 
GTTGGAGGGA GATCATGTAA ATCGTGGAGA TCAAGAGGAG GAGAGACACC AAATTCGGGT 
TCCATAAGCA GCTCGGGTCA GATGTCAATT CAAGTGTCTG GTCTGAGTGG ACCATCGGGA 
AAAGAGTTTC AGGAAAAACC ATTAACACTT GTAGAGTTCG CTGAACTTTT TCGATTGTTC 
AATACAAGAA TGAGAAAAGA TTTGAGAGAT GTGTTCAATG ATGTCCTGTC TACGGCAACT 
ACTCCTCAGC ATTGTCCTAA AAGAGAACGT GATCGCCACA GTCCGAGAAT GCAGTCAAGG 
CTGGCGAGTG TCTCCAACTC GTACAATGCA GATTTTCTGT CTAACGATTT CTTGACTCGG 
AATACGGCAG TAACGTCCCA TCATATCAGT GAAAAGCAAA ACAAAATCTA CAATGCATTG 
GCACTTGCGA GTGTTAATAG CATGGGCGGA CTCATGGACA CTTCCCGGTC GTCGATGCTG 
ACGCCGCAAA TGCTGCGCGC TTTTGTCAAC ACTCATCAGA TGGAGCAGAT TGACGAGCAA 
ACGGCGATTA AGCTTATTCA GGATCATGAA CCTGATGGTA TCTGTCGACA AAAGAATCAA 
ATGTCATTCG AAGGATTCAC TAGATTCCTT TGCGATCCTG TCAACTTTGC ATTCGTACCG 
GAAACAATTG AACCAGATGA AGAGGACCTA CGGTACCCGT TGTCTCATTA TTACATCAAT 
TCAAGTCACA ACACCTATTT GACGGGACAT CAACTCAAGG GACCCAGTAG CTCGGAGATG 
TACAGACAGG TTCTCCTCAC CGGGTGTCGG TGTGTCGAGC TTGATTGTTG GGATGGAGAT 
GACGGATTGC CATTGATTTA TCATGGACAC ACATTGGTTA GCAAGATTGG GTTTAGACAG 
GTGGTCGAAA TTATCAAAAA ATCTGCATTC ATAACATCGG ATCTTCCTGT GATATTATCA 
ATTGAGAATC ATTGTTCCCT ACAACAACAG GCGAAAATGG CACAGATGTT CAAAACAGTT 
CTTGGAGATT TACTAGTATC AAACTTCCTT TTTGAAGCTG ATTTCTCGGA CTCTCCACGT 
CTTCCATGCC CTCTCCAAAT GAAAAATAAG ATCTTGATAA AAAATAAGAA AATGATAGTC 
GACCCACCAA CTCCATTGCC AATGATTGAA CGAGGAGCTG TGCAGCGTGG TGAAACTCAA 
TTAAATCTTC ACAGAAAACA GAGTAAAAAC TCCTACGAAA GCAGTACGGT AGATGAAGTG 
GAAGATGACG ACCTAGATGA GTTTCTTGAC GATGAAGAAA ATGAGGAGGA TGACCAAGAA 
GAGGTTCAAG TCAGAAGTGA AAAAGAAGAC AGTCCGAAGA CATCAAAACG TGCAGAGAAA 
TCGGCTAGAA ACATCAAACA GCAAGATTCG TTATGTAGCG ATCATTCGGT AGAGCAGGCA 
AAACCTTCCA CAAGCAAGAC CACATCCAAA ACCAACGATA GAAAGACTGA AGATGAAGTA 
CTTTACGCTC AACTTGCTCA AAATGCCATC CGAAACCAAC AACCAAGAAA GAACAATACT 
GGAGTCCAAA TAGCGCCAGA GTTGTCAGAT ATTGTCATTT ACATGCAAGC GACAAAATTC 
AAAGGATTTC CCCCAGTCGA TGGCATTCAA TCACCAAGGA TAATGGAGGA AGGGCCAGCT 
TCTGCAAGTC TTTCATTTTC ATCACGGGCT CGTACGCCAT CAAATCTGCT GAATACACCA 
GCCCCTCCAC GACGACAGCG AAGTAGCACT CAGTTGAGTC AAGAACTGGC TGCAGAATTT 
TTGGGATCAG TTAGGGCAAA TGCTACAGCT ACATGCTACC AGGTTACATC TCTTAATGAA 
AACGCTGCAA AGAAACTGAT GAAAAGACAT CCAGCAAAAT GCGTATCTTA TACTCGGGAC 
CATCTGATTC GTACGTATCC AAGTGCCAAG CACTATGACT CGTCCAACTT TAACCCCATC 
AACTGCTGGG CTCATGGAAT GCAAATGGTT GCTTTAAATT TTCAAACTCC TGATGTTATA 
ATGGCAGTTA ACCAAGCGAT GTTCGAGCAA TCTGGAAACT GTGGATATCA ACTGAAACCA 
CGTTGTCTTT GGGATGAATC TCATCTTTTG TACAACAAAT TTTTGCCTCT CTCCAAAGAC 
ATTGCCGGCC ATTCTGCTTT GTTACTAAAC TTAACTATTA TATCCGGACA GCACGTGTAT 
CCAAACACCC ATTACGCCAG TTTATATGTC GAAATTGAAG TCATCGGTAT TCACAACGAC 
TGTGTTCGTG AGAAATCAAA AGTGGTCCAG AGAAATTCTG TGAATCCAAT CTGGAACCAC 
ACCACTCAAC TTCGAATAGC TTGTGTTGAC TTAGCATTCC TTCGCATAGC AGTATGTGAT 
AGTGGGCAAA ATGGAAGAGT CGTTGCTCAC CGAGTTGTTC CTGTCAAATG CATTCGTCCG 
GGTTTCCGAC ATCTCCCACT CAGAACTCCA ACTAATCTTC CGATCGACAA CGCTATGATA 
TTTTTAAGAA CAAGATTCGA GCAAGAAGAA CACATCTACC TCCACGACGA TGATTCAAAC 
ACCTATTGTA ATCTAGAACA TACGCTGGCA TACCGCACAG ACTTAACACC CAATTTATCT 
CCAACGCCAA TTTTGAAGAA ACAGATCTTT GTTCTTCGAA TTACAGGCGC GTTTGCTGAT 
GAAACAGCAA TTACAGTGCA CAGCGAGTCA GGAAGTACAG TGAAGACGGT GATGCAACAG 
GCGCTGCTTA ACGCTGGAAA GAATGCGGAT CAGGTTGAAG AGTATGTACT CATCGAAGAA 
TCACTGCCGG CACCTTCTGG GGAAGATCCA ATTGAGCAGA GAGTACTTCC TCTGAACGAG 
CCTATCATGG ACGCCGTGGC CTGCTGGAAC GGTTCAATGC GTCGTTTTGT GTTACGAAAA 
AAAGGCAGTG ACCCATCATC TAGAGCTTGG ATCACTTCAA TTATAAAATC CGGAACAAGT 
GGAAGCTCCA CGTCAGTCTC ACCAAGTCCG TTGACCAAAG ATGGACACGT AAAGAGTGCA 
TCAAGCAATC AACTTCATGG ACGATCATTG GACACTGACG CTTTTGGAGA GCACCTTGAA 
GTGACAGAAG GGTTGAACCC TCGTGCAAGA TCAATGGGCG ACACATTTCT GGTATGTGTT 
CACAATGTTA GTGAAGATCA GCCTTATGCA ATCCTACGTG CTGGAATCCA CAGCACTGCC 
GCCGACATTA TTCGTCAGGT GTTTGTTAAA GCTCGCCGTT CAAATGTAGA CGATTCCGAG 
TTTGTTCTTG TTGAAGAAAC CTGCGATGAT CCAAAACTCA ATCAAGGCCA AATGACTCCA 
AAGTATCCAA ACAACCGCAC CACAAGCCGA GTGTTAGGAC AAAACGAAAA TGTTTGGAAA 
GCACAATCGA GGTGGAAGTC AATGGGCAGA TTTGTACTTG AAAACCGAAA GGACACAGTG 
CACGCAACCC TCGAAAAAGT GAGATCATTT ATATCAAAAC TGGAAGCTGC AAAAGCAAGC 
ATGGATCCCA GACACGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129927.1
CDS	1..5661
Translation	MPHDSKMTSVVSRLLHALGKSHDHEEPETPPNPDSLLVYVCKLATSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKVRSFISKLEAAKASMDPRHE

Target ORF information:

RefSeq Version	NM_001136455.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Phosphoinositide phospholipase C (plc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136455.2

ORF Insert Sequence:

ATGCCTCACG ATAGTAAAAT GACATCGGTT GTGTCCCGTT TGCTTCATGC TCTCGGAAAA 
TCTCATGATC ATGAAGAACC TGAAACTCCG CCTAATCCCG ATAGTCTTCT TGTATATGTG 
TGTAAATTGG CAACATCACG TAGTGACTCA ACCTCTTCAA CGCAATCAGC CACAGCTGGA 
TTCATTCTCA ATGAAGAACC AATCACACTG TTCCGACTTG AACTCGAACG ACTTCAATAT 
ATTCTTCATT TCCCTGAAGA AGTTGCATTC CAACTATCTT CTACTGAGTA TCAGCTTTTC 
TACTCAATAC AACCCATGGA TTATGTTCGT TACGTCAGTT GCGATCTCAC ATCTGTTCCA 
GTGTCAGAAA ATCCAAGTCC AGTGCGAAAT CTTGTGAAAA GGCTATCAGA GGTATCATCC 
TGGATAACAC ATGTTATTGT CAGTCAGCCA ACACATGACG ATCGAAAGGT TGCTCTTACT 
GCAATTTTGA GAATTGTGGA AACTTGCTGG AATATTGGAA ACTTTAATGC AGCTGTTGAG 
GTTCTTATGG GTTTAAAGAG TGAAAAACTC AGACCATTCT GGCTTTCTTT GCGACAAGAG 
GAAAAGTCTC AGTTTGATTC GCTTTGCGAA ACCTTGCTTC CTGCAAACCA AGCTCTCCCA 
TCTCAAGCTT ACATCAACGC GGTCCAACGA GCACTGAGAA TGCCACAATC TCGTGTGATT 
CCATTTTTTG GAATATTTCT TCGTGATCTC TACGCAATTG TGAATGACTT GCCGAATATC 
GTTGTGATTG GACAAGAAGG TGAAACCCAA AAGCTGGAAT TCATGAGCGA TCCCAATGGC 
GAAGATCATT TTTCGTCGAG AATTGGAGTG GGTGGGCTGT TGAATGCTGA TAAAATCAAC 
TTGGTGGCAA TTGTTTTGGA CAACTTGGAA TTATTCCATC GTCACAGCAG GACAATGATC 
AAATTGTTAG AAGAGCAAGC AGTCCCACCA ATTCAAATTC CTCAGAACGA GCGGGAGCAA 
AAAGAAAAGG AAGCAAAAAC ATATGAACCA GTACAAGTGG TTCGCGGCTC TTCTCATGGA 
GTGGCTCTCA TCCCTCTTGA TACCCTAACA TTCGATCTTG ATGTTATCCA ACGACTCCAG 
CACGGAACAA CAGTGATCCA TTACGAACCT GATTCGGGAA GAAGCAATTT GTGTCTGCTG 
CGGTTAGACC CTAGTTGCGG TCAAATAAAC TGGCATAAGA TTTCATATTC AGTTAACAAA 
GATCCCAAAG AAAAGGATGT GCTCGCCAAA GTATCCGTTT CAAATCTGCA GCCGCTAGAC 
TCTGGCCGAG GGGCTCCGTC ACCAATGCCA TCTGGAAGAA CACCAGGAAC TGGAGGAGTT 
GGAGTAGAGG AGGGAGAACT CAAATTAAGT GTTGTGAAAG GAGTCGAACT AGTTGATAGT 
TATGATATTG ATATTGAGGC AATTTACCGT CGTCATTCTA TGGAAGAGAT GTCAGTTCCT 
GTTAGCTGTT GGAAAGTTAG TCATGGTCAG TTGTTATCGG ATAATGAGTT CATCTACTTT 
TTGGCCCCTC AACAAATTGC TCAATTTTGG ACCAACGGGT TGCAATCTGT AGTTAAAAGT 
CTCCAAGGAC AGCAAAGGTA CCCTGATCGG CGAATGTTAT GGATCAAAAA TGTCTACTTA 
TCACTTTACG AGATAACGGG CGAATCAAAC TGTGGACCAC GGCCATTTGA AGCACTTCAG 
GCATTTGGGC TGAGTCAAAC AAATACTAAC GCAACACGAC CCAATGACTC GTCGCTGTCC 
AGTGAGCCAG GAGGAGCTAA AAGCAGACTG AAGAACCTTA AAAATGCTAT GCAAAAGAAG 
CTTCGTGGTG CATCGCGCGA AGGAAGCAGA TCTCAGTCAC CTCAACCTCA TTCTCCTCTT 
GTCCGACCAC CATCAATAAA ATCGCAAATA TCGTCACAAT CTGGTCCGCC GGGTCCGAAC 
TCTCCAGGCT ATTTGCTGAA ACCACGTGGA GAACCTGCCA ATAGTGATGC AGGAGACATT 
GATAGCATTT ATACACCTCG ATCAAGAACT CCAACAAGCA GCTCCTATGG AGGGCGATCC 
GTTGGAGGGA GATCATGTAA ATCGTGGAGA TCAAGAGGAG GAGAGACACC AAATTCGGGT 
TCCATAAGCA GCTCGGGTCA GATGTCAATT CAAGTGTCTG GTCTGAGTGG ACCATCGGGA 
AAAGAGTTTC AGGAAAAACC ATTAACACTT GTAGAGTTCG CTGAACTTTT TCGATTGTTC 
AATACAAGAA TGAGAAAAGA TTTGAGAGAT GTGTTCAATG ATGTCCTGTC TACGGCAACT 
ACTCCTCAGC ATTGTCCTAA AAGAGAACGT GATCGCCACA GTCCGAGAAT GCAGTCAAGG 
CTGGCGAGTG TCTCCAACTC GTACAATGCA GATTTTCTGT CTAACGATTT CTTGACTCGG 
AATACGGCAG TAACGTCCCA TCATATCAGT GAAAAGCAAA ACAAAATCTA CAATGCATTG 
GCACTTGCGA GTGTTAATAG CATGGGCGGA CTCATGGACA CTTCCCGGTC GTCGATGCTG 
ACGCCGCAAA TGCTGCGCGC TTTTGTCAAC ACTCATCAGA TGGAGCAGAT TGACGAGCAA 
ACGGCGATTA AGCTTATTCA GGATCATGAA CCTGATGGTA TCTGTCGACA AAAGAATCAA 
ATGTCATTCG AAGGATTCAC TAGATTCCTT TGCGATCCTG TCAACTTTGC ATTCGTACCG 
GAAACAATTG AACCAGATGA AGAGGACCTA CGGTACCCGT TGTCTCATTA TTACATCAAT 
TCAAGTCACA ACACCTATTT GACGGGACAT CAACTCAAGG GACCCAGTAG CTCGGAGATG 
TACAGACAGG TTCTCCTCAC CGGGTGTCGG TGTGTCGAGC TTGATTGTTG GGATGGAGAT 
GACGGATTGC CATTGATTTA TCATGGACAC ACATTGGTTA GCAAGATTGG GTTTAGACAG 
GTGGTCGAAA TTATCAAAAA ATCTGCATTC ATAACATCGG ATCTTCCTGT GATATTATCA 
ATTGAGAATC ATTGTTCCCT ACAACAACAG GCGAAAATGG CACAGATGTT CAAAACAGTT 
CTTGGAGATT TACTAGTATC AAACTTCCTT TTTGAAGCTG ATTTCTCGGA CTCTCCACGT 
CTTCCATGCC CTCTCCAAAT GAAAAATAAG ATCTTGATAA AAAATAAGAA AATGATAGTC 
GACCCACCAA CTCCATTGCC AATGATTGAA CGAGGAGCTG TGCAGCGTGG TGAAACTCAA 
TTAAATCTTC ACAGAAAACA GAGTAAAAAC TCCTACGAAA GCAGTACGGT AGATGAAGTG 
GAAGATGACG ACCTAGATGA GTTTCTTGAC GATGAAGAAA ATGAGGAGGA TGACCAAGAA 
GAGGTTCAAG TCAGAAGTGA AAAAGAAGAC AGTCCGAAGA CATCAAAACG TGCAGAGAAA 
TCGGCTAGAA ACATCAAACA GCAAGATTCG TTATGTAGCG ATCATTCGGT AGAGCAGGCA 
AAACCTTCCA CAAGCAAGAC CACATCCAAA ACCAACGATA GAAAGACTGA AGATGAAGTA 
CTTTACGCTC AACTTGCTCA AAATGCCATC CGAAACCAAC AACCAAGAAA GAACAATACT 
GGAGTCCAAA TAGCGCCAGA GTTGTCAGAT ATTGTCATTT ACATGCAAGC GACAAAATTC 
AAAGGATTTC CCCCAGTCGA TGGCATTCAA TCACCAAGGA TAATGGAGGA AGGGCCAGCT 
TCTGCAAGTC TTTCATTTTC ATCACGGGCT CGTACGCCAT CAAATCTGCT GAATACACCA 
GCCCCTCCAC GACGACAGCG AAGTAGCACT CAGTTGAGTC AAGAACTGGC TGCAGAATTT 
TTGGGATCAG TTAGGGCAAA TGCTACAGCT ACATGCTACC AGGTTACATC TCTTAATGAA 
AACGCTGCAA AGAAACTGAT GAAAAGACAT CCAGCAAAAT GCGTATCTTA TACTCGGGAC 
CATCTGATTC GTACGTATCC AAGTGCCAAG CACTATGACT CGTCCAACTT TAACCCCATC 
AACTGCTGGG CTCATGGAAT GCAAATGGTT GCTTTAAATT TTCAAACTCC TGATGTTATA 
ATGGCAGTTA ACCAAGCGAT GTTCGAGCAA TCTGGAAACT GTGGATATCA ACTGAAACCA 
CGTTGTCTTT GGGATGAATC TCATCTTTTG TACAACAAAT TTTTGCCTCT CTCCAAAGAC 
ATTGCCGGCC ATTCTGCTTT GTTACTAAAC TTAACTATTA TATCCGGACA GCACGTGTAT 
CCAAACACCC ATTACGCCAG TTTATATGTC GAAATTGAAG TCATCGGTAT TCACAACGAC 
TGTGTTCGTG AGAAATCAAA AGTGGTCCAG AGAAATTCTG TGAATCCAAT CTGGAACCAC 
ACCACTCAAC TTCGAATAGC TTGTGTTGAC TTAGCATTCC TTCGCATAGC AGTATGTGAT 
AGTGGGCAAA ATGGAAGAGT CGTTGCTCAC CGAGTTGTTC CTGTCAAATG CATTCGTCCG 
GGTTTCCGAC ATCTCCCACT CAGAACTCCA ACTAATCTTC CGATCGACAA CGCTATGATA 
TTTTTAAGAA CAAGATTCGA GCAAGAAGAA CACATCTACC TCCACGACGA TGATTCAAAC 
ACCTATTGTA ATCTAGAACA TACGCTGGCA TACCGCACAG ACTTAACACC CAATTTATCT 
CCAACGCCAA TTTTGAAGAA ACAGATCTTT GTTCTTCGAA TTACAGGCGC GTTTGCTGAT 
GAAACAGCAA TTACAGTGCA CAGCGAGTCA GGAAGTACAG TGAAGACGGT GATGCAACAG 
GCGCTGCTTA ACGCTGGAAA GAATGCGGAT CAGGTTGAAG AGTATGTACT CATCGAAGAA 
TCACTGCCGG CACCTTCTGG GGAAGATCCA ATTGAGCAGA GAGTACTTCC TCTGAACGAG 
CCTATCATGG ACGCCGTGGC CTGCTGGAAC GGTTCAATGC GTCGTTTTGT GTTACGAAAA 
AAAGGCAGTG ACCCATCATC TAGAGCTTGG ATCACTTCAA TTATAAAATC CGGAACAAGT 
GGAAGCTCCA CGTCAGTCTC ACCAAGTCCG TTGACCAAAG ATGGACACGT AAAGAGTGCA 
TCAAGCAATC AACTTCATGG ACGATCATTG GACACTGACG CTTTTGGAGA GCACCTTGAA 
GTGACAGAAG GGTTGAACCC TCGTGCAAGA TCAATGGGCG ACACATTTCT GGTATGTGTT 
CACAATGTTA GTGAAGATCA GCCTTATGCA ATCCTACGTG CTGGAATCCA CAGCACTGCC 
GCCGACATTA TTCGTCAGGT GTTTGTTAAA GCTCGCCGTT CAAATGTAGA CGATTCCGAG 
TTTGTTCTTG TTGAAGAAAC CTGCGATGAT CCAAAACTCA ATCAAGGCCA AATGACTCCA 
AAGTATCCAA ACAACCGCAC CACAAGCCGA GTGTTAGGAC AAAACGAAAA TGTTTGGAAA 
GCACAATCGA GGTGGAAGTC AATGGGCAGA TTTGTACTTG AAAACCGAAA GGACACAGTG 
CACGCAACCC TCGAAAAAGT GAGATCATTT ATATCAAAAC TGGAAGCTGC AAAAGCAAGC 
ATGGATCCCA GACACGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_001373494.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001359720.1 Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

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CloneID	OCa851220
Clone ID Related Accession (Same CDS sequence)	NM_001373494.1
Accession Version	NM_001373494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on the 5' end.

ATGTCCTTAC GCCAAGCATC ACACAAATTT CCATCGGTGG ACTCAATGCC AACATCACGG 
GATTCATCGA TGGAAACAAC CGACCGGTAC CCGTTTGGGT ATACGACCGA ATTGGCACGT 
ACCGAACCAT CTGCATCAAC TGCCAAACGT CTTCGACAAA GGTTACAGCG ACAGCAAAGT 
GGTCAATCAG GATTATCCGG TCGACGGTAT ACTGGAGAAA GCAGCGAGTG CTCATCACTT 
GTCGGAACAC CCACAGACAC TGGCAGATCA TTTACATTTG GAGCAGCACA AAGAGTCATG 
CGGGAACGGG ATGACCGCAA ACCATCAGTT ACCCAGAACT TGTCAGAAAT AATGCTCGAG 
GCAATACATT ATGAAGATGT GAACTTGGTG GAAAGGTTGC TCACTGCGCA TAGTTCAAAA 
CAACCAATGT CAACATCTCC GTCAATTGGT AGCACAAACA CATTGACGGA TCTTGCTCAA 
CGTAGACACG GTGCGGGGGC ACACCGACGT TCCAATGCCA GTAGTACGGT GTCCACACAT 
TCCGGAGGAA GATCCACGTG TGCTATGATG AACACGTTGC ACATGGCTGT GGCTCATAAA 
CAACGAGATA TAGTGGAACT TTTGCTTAAA AACGGCTACG ATCCCAATAC TCCCGCATCG 
TGTCACTGTA AGGGAAATTG CACGGCGACT GGGAATATTC CACTGACTTC GATCATTCCG 
AGAACTCATT CAATGACGCC AGAATTGTGT TCGACGTGCT CTCAACTGAG GGTTGTGTCA 
ATCGTGGATC AAACACCGCT TGGTGTGGCA GTCAGATCAC AGTCCAGCGA ATTGATAGCA 
TTGTTGATTG CCTACGGAGG AGATGTTAAT TTAGGTGATG AAGATGGCAA CACGCCGTTG 
ATGCTAGCCG TACGAGAATC ACCTCTGTCT TGGCCTTGCC TGCACACGCT CATTTTCTTT 
GGAGCTCAAA TTGAACAAAA GAACATGCGC GGAATATGCC CTCTTGATCT TGCTCCAGAG 
CTGAAGAAAC TTCAACAAAC TTGCGTTGAA GAGCTTTTCA AAAATGCGTG CTCGGGTGAT 
TGTGAACCAC CATCTCGATC TACACCGGGA CCATCAGCCA AAAACTGGAA TAGACTACAA 
GTTGACAATG TTTCCCAAGG AGAACGATCA CTGTCAAAAG CTCCATTGAG CCCAAAACCG 
TCTGCTGCAG CAAGTGTCTC AACGTGTAGC ATGCTGGAAA CCTCATCAGC AAAAGAATGT 
GCACGACGAA AATCATTGGT GTCGCTTCAA CTTCACAGAA AAAGCCGAAA CGTGAAAGAA 
TATCCTGTAA TTGACTGCGT CAGTTGGGAA CAAGCATGGG AATTGCTGAA GAAGATGGCA 
TGTAATCCAG AATGTCTAGA TAACATCGTG AATTCATTGT CAAAATTTGC AACCCAAATG 
GAGAACACCC CGTCAAGCGT TGATCGTGAT CAATTCGATG CACACATGGG TGGCCTACTA 
CATAAAATGA TTCATACAGC GATTGAGGAG TACGAGTCTT CGACACCAAC CTACAAAAAG 
CAGAAGAAAC TCCATCTCAC CTGGCTGCTC AGCCAAATTG CCACGTTTTG TTTTACATTT 
CTGCAGAAAA GTGGAACGAG CCGACAATTC TCAGCACTGA ACACGTTGAA TAAAATTATT 
GATGCTGGTT TAGTACATGA TTTGTTCGCA TTCAACGACG TTGTCTTTCA TTCTTCAAGA 
ATTTTGAACC GATGCCATGC ATTTGATTCC GACGAACCAC CAACTCCATC TGGCGAATCT 
TTCAACTCAA ATGCAGACCA AAACTCAACT GTCGATCATG TTTTCATTTA CGGAGCTGGC 
TATAAAGACC AATCGTTATT ACCTTCTCCT ACGGTGGCAC CAACTCGACA CAAGTCCGAC 
TTAATGAATT CTTTCAGCGA AATGGATCCA AGCCAGGTCA TTGTGGCCTT GCACAACGCG 
ATAACAATGC AAAACCGGGA AGTAGGGTCA AGATCTGTTT GTAGTCCGGC TCATCGATGG 
AGGCAGTGTT GTGCTCATTG TACACAGATA TTGGTGGCCA GATTATTGCT ATTCTTAACA 
CACGCAAAAA AATTTCGAAG TCGGTTGTCT GAACGACATC AGCTCAGAAG CTTGGTAGCT 
TTGTTAGAAC CAACTTTGGA ACCTCAACTC TTATGTCTTT TACTTCAAGT TCTAGCTCTT 
ATAGCGTTAG ATCCATCTAC CCACACAACT TTGATAGACA TTCAAATTGA CGACGTCTTG 
ATTCAAATGC TACTCCCAGC AGATGATTGG TACTACACTA ATCACAGCAC TAAATATGGA 
ACTTTTGTAA AGTACCATGC TGCTAGAGTT TTAGTGTACG TTGGAATGGG TGATCGAGTA 
GGAAGTCGAG TCAATCTGTT CAACATGATG ACGTTTGAGC CATCTGCAAA AAGCGGGAGT 
CAACAAAACG AGGACGACTA CATTTGTGAG ACATGTGCCA CACCGCGCAG CCGAAGTACA 
TTTTCAAGAT CAGCAGTTTC AGTGGAAGGT GTTCTGTTAA AAGTGTTGGC AGAAGTTGCT 
GAGCTTGTAC GTCAGAGTCA ACACCTATCA GCAAGTGAAC CAATAACTGA AGAATCGCCC 
AGTGCAGGAT CTCCACCCGC TGTCCTACCA AATCAGGCTG AACAGAAAAG TATTAACGAA 
AAAAATGCAG AGAAACTTAC ACAACTTGGG TGCCAAATTT TGATCTCATT GGAACAACTT 
GAAGCTCATT TATGCAAACT TGGACTGGTA CTTGATTCGG TTCTACTTCT CCGTCTACTT 
CTTCATAAGT TGTCATGGGA TCTTGGATTA GTCACAAAAA AGAGGGTTGC TGTGGTGGAT 
CATGTTTACA AACCAATCAC GGATCCGAGA GCTCATTCTA GCTGCAGTCT TGGAAACAAC 
AGGCGAGAAG ATAGCAAACG GGACAAAAAT TACTTGCGTG TTGATCAGGG ATCTCGTGCG 
TCAAAGAGAG TGCATATCAG ACGATCTAGT TCAGTAGAGA TTATCAGGCC AAAACGACTC 
AGTTCCGGAG CAAAAGATAT CAAAAATCGA GAACGGAGGA AAAGGCTGGG AACAGATACA 
AGTAGCGGGT CGAATCGTTC CAAGAAAACC AACTCCTCGT CTTCATCTGT TCAGAAACAC 
TTGCCAAAAT ACATTCAATC ATTATTCCGA AGTCGGATGG GAACTGATCC ATGCAAGAGG 
ACATCGAGAA GTGAATCCAC TTTACCGCAC AGAGACTCTA ATGAGAGTAA TACAAGTGGA 
AGTGATGCTG TTCTTGAGTT CACAAGAAAA CTTCAAAATT ATCCGTTGAC GAGACGCGAA 
GCGCAAAGAT TGACGTATCG AAGCAATGGG CAACAAGATT CAAATGACTC GAAGATGGGA 
CCACGAACTT TAGGATACGT TTACCTCCCA GAACTAGAAA TTCAAGGTGC AAGTCCGCCA 
AGATCACCGG GACCTCTAAC TGGAAACTCG TTAGAAGAAG GAACCACAAT GCTTTTGGAC 
ACAAGACGGC CGAGCAGCCC ACAAGCAATC CCCGGCCTTC CTTTAATTGA AATTCGTCGG 
CCCTCAGCTC TCTCTCAATT TGAATTTGGA TACTTTGTAA ACTCACCAGA ATTATCAGGA 
AGTGAAGTAT CTGATTGTGC CCCATTACTT CTCAGTGGTC AAATTGGTGG AAGCCGAAAA 
AGCTCCGACG AAACATCATT GGGAGGATGG TCCAGCCGTG CTTCATCGGT AATGAGTCAA 
AGGTCGTCCA GATCCAGTGT TGGCTTGAGG TTATCAACAT TTTCTGGAGG GACGAGTATT 
GCTTCGGATA ACTCTGGGCC ATTTTTGTTC AGTTTTGTTT TGAGAAAACG TGCATCTACT 
ATAGGAACTA GAATACCAAT ACCTAGGAGA GCTTTGTCAA GATCTTCAGG AGACAGTCTG 
AGAGTACCTG ATAGAGAAAG TCCACTTCAT TTAGCAAGTA TGACAGAGAT GAATCCGGAT 
TTTCAATGTG TTCGACAGCT CATCTTGAAC CTTTTGAATG TTTACACGAA AAGAAATGAC 
AATGTGGTTT CTACAATGAA GGAATGTGCG GATGTTCTTC GTCAAATCCT AAACTCTCCT 
CAACATCCAA CTGTCAAGAA TTGGTGTGCC GAAATAATTC ATGTAGTCAG TACTCATGTA 
GAAGAAGAGG AACCAACGAC GGCAGAAAAT AATGAACAAA TTAATGACGA GTATCTGGAA 
TTTCAAGACC AAGTGATATC AGGAAGTTTA CCATGCCCGA AGGAAGAGGC TGCATACCTT 
GCAAGTATTC AGTTATCAGT TGAAGAGCAG TGGCCGAGTA ATAAAAGGAC CCAGACAATT 
AGAAGACATT TATTGAAAGG ACAGTTTGGT CGAATCCGGG ATCTCGCCCA AAAGATCATG 
GTGACTCCAT GGGAAGTTGA TCAAAACTTG TACTGCACAC CACCCAGATT TCCCAATGAA 
TCGGCAAATG CTAGTAGAGC ACAATCAGTG GTTGAGGAAA TTCAGCACAG ATCAAGAACT 
CCAACTCTTC TGCGATGTAT CACAAACACA GACGGCTTAA TGTCAGAAGA GATGCAAGCT 
CAGTGCCTTC CCGTCGATCT ACGTGGTGAT CGTAGAACTA TCAAGCTGGT CAAGGAGAGG 
AAGCGAAAAT TGTTCCATAG TCAAGTCTAT GAAAGTGAAA TTGGCATGAA GAAATTGTAC 
ATTCAGACCG CCAAAAAACT AGCCGCATTT GGTTGTAAGG TTTTTCAAGT TAAAGAGTTG 
CTTCACGGAC GAACACTCAG AAAAACTCTT CGACTGCTTT GCCTTTCTTC TGCTCAATTG 
TGTCTTCTCG ACGGATCAAC AAAACTTGTG CTTAAACGGC AGCACGCATC GACGTTACAA 
CAGTGGAGAG TAGGTGGTGG AGTTAGTAAG CATCAACTAT TGCTCGAATT CCGTGGAACC 
AAGTGGCAAC TTATTGCACC GTCGTATAAT GCACTGAAAT CGATATCGAT GACATTATGG 
GAGATTATGC AAAATTCTGC TAGCAATTCA ATCCAGCGGA GTCTCAACCA GTCAATGCAT 
CGTTCGCACA CCGAGGCGAC TGTCTCAACA TCACGTAGTG ACTCAACCTC TTCAACGCAA 
TCAGCCACAG CTGGATTCAT TCTCAATGAA GAACCAATCA CACTGTTCCG ACTTGAACTC 
GAACGACTTC AATATATTCT TCATTTCCCT GAAGAAGTTG CATTCCAACT ATCTTCTACT 
GAGTATCAGC TTTTCTACTC AATACAACCC ATGGATTATG TTCGTTACGT CAGTTGCGAT 
CTCACATCTG TTCCAGTGTC AGAAAATCCA AGTCCAGTGC GAAATCTTGT GAAAAGGCTA 
TCAGAGGTAT CATCCTGGAT AACACATGTT ATTGTCAGTC AGCCAACACA TGACGATCGA 
AAGGTTGCTC TTACTGCAAT TTTGAGAATT GTGGAAACTT GCTGGAATAT TGGAAACTTT 
AATGCAGCTG TTGAGGTTCT TATGGGTTTA AAGAGTGAAA AACTCAGACC ATTCTGGCTT 
TCTTTGCGAC AAGAGGAAAA GTCTCAGTTT GATTCGCTTT GCGAAACCTT GCTTCCTGCA 
AACCAAGCTC TCCCATCTCA AGCTTACATC AACGCGGTCC AACGAGCACT GAGAATGCCA 
CAATCTCGTG TGATTCCATT TTTTGGAATA TTTCTTCGTG ATCTCTACGC AATTGTGAAT 
GACTTGCCGA ATATCGTTGT GATTGGACAA GAAGGTGAAA CCCAAAAGCT GGAATTCATG 
AGCGATCCCA ATGGCGAAGA TCATTTTTCG TCGAGAATTG GAGTGGGTGG GCTGTTGAAT 
GCTGATAAAA TCAACTTGGT GGCAATTGTT TTGGACAACT TGGAATTATT CCATCGTCAC 
AGCAGGACAA TGATCAAATT GTTAGAAGAG CAAGCAGTCC CACCAATTCA AATTCCTCAG 
AACGAGCGGG AGCAAAAAGA AAAGGAAGCA AAAACATATG AACCAGTACA AGTGGTTCGC 
GGCTCTTCTC ATGGAGTGGC TCTCATCCCT CTTGATACCC TAACATTCGA TCTTGATGTT 
ATCCAACGAC TCCAGCACGG AACAACAGTG ATCCATTACG AACCTGATTC GGGAAGAAGC 
AATTTGTGTC TGCTGCGGTT AGACCCTAGT TGCGGTCAAA TAAACTGGCA TAAGATTTCA 
TATTCAGTTA ACAAAGATCC CAAAGAAAAG GATGTGCTCG CCAAAGTATC CGTTTCAAAT 
CTGCAGCCGC TAGACTCTGG CCGAGGGGCT CCGTCACCAA TGCCATCTGG AAGAACACCA 
GGAACTGGAG GAGTTGGAGT AGAGGAGGGA GAACTCAAAT TAAGTGTTGT GAAAGGAGTC 
GAACTAGTTG ATAGTTATGA TATTGATATT GAGGCAATTT ACCGTCGTCA TTCTATGGAA 
GAGATGTCAG TTCCTGTTAG CTGTTGGAAA GTTAGTCATG GTCAGTTGTT ATCGGATAAT 
GAGTTCATCT ACTTTTTGGC CCCTCAACAA ATTGCTCAAT TTTGGACCAA CGGGTTGCAA 
TCTGTAGTTA AAAGTCTCCA AGGACAGCAA AGGTACCCTG ATCGGCGAAT GTTATGGATC 
AAAAATGTCT ACTTATCACT TTACGAGATA ACGGGCGAAT CAAACTGTGG ACCACGGCCA 
TTTGAAGCAC TTCAGGCATT TGGGCTGAGT CAAACAAATA CTAACGCAAC ACGACCCAAT 
GACTCGTCGC TGTCCAGTGA GCCAGGAGGA GCTAAAAGCA GACTGAAGAA CCTTAAAAAT 
GCTATGCAAA AGAAGCTTCG TGGTGCATCG CGCGAAGGAA GCAGATCTCA GTCACCTCAA 
CCTCATTCTC CTCTTGTCCG ACCACCATCA ATAAAATCGC AAATATCGTC ACAATCTGGT 
CCGCCGGGTC CGAACTCTCC AGGCTATTTG CTGAAACCAC GTGGAGAACC TGCCAATAGT 
GATGCAGGAG ACATTGATAG CATTTATACA CCTCGATCAA GAACTCCAAC AAGCAGCTCC 
TATGGAGGGC GATCCGTTGG AGGGAGATCA TGTAAATCGT GGAGATCAAG AGGAGGAGAG 
ACACCAAATT CGGGTTCCAT AAGCAGCTCG GGTCAGATGT CAATTCAAGT GTCTGGTCTG 
AGTGGACCAT CGGGAAAAGA GTTTCAGGAA AAACCATTAA CACTTGTAGA GTTCGCTGAA 
CTTTTTCGAT TGTTCAATAC AAGAATGAGA AAAGATTTGA GAGATGTGTT CAATGATGTC 
CTGTCTACGG CAACTACTCC TCAGCATTGT CCTAAAAGAG AACGTGATCG CCACAGTCCG 
AGAATGCAGT CAAGGCTGGC GAGTGTCTCC AACTCGTACA ATGCAGATTT TCTGTCTAAC 
GATTTCTTGA CTCGGAATAC GGCAGTAACG TCCCATCATA TCAGTGAAAA GCAAAACAAA 
ATCTACAATG CATTGGCACT TGCGAGTGTT AATAGCATGG GCGGACTCAT GGACACTTCC 
CGGTCGTCGA TGCTGACGCC GCAAATGCTG CGCGCTTTTG TCAACACTCA TCAGATGGAG 
CAGATTGACG AGCAAACGGC GATTAAGCTT ATTCAGGATC ATGAACCTGA TGGTATCTGT 
CGACAAAAGA ATCAAATGTC ATTCGAAGGA TTCACTAGAT TCCTTTGCGA TCCTGTCAAC 
TTTGCATTCG TACCGGAAAC AATTGAACCA GATGAAGAGG ACCTACGGTA CCCGTTGTCT 
CATTATTACA TCAATTCAAG TCACAACACC TATTTGACGG GACATCAACT CAAGGGACCC 
AGTAGCTCGG AGATGTACAG ACAGGTTCTC CTCACCGGGT GTCGGTGTGT CGAGCTTGAT 
TGTTGGGATG GAGATGACGG ATTGCCATTG ATTTATCATG GACACACATT GGTTAGCAAG 
ATTGGGTTTA GACAGGTGGT CGAAATTATC AAAAAATCTG CATTCATAAC ATCGGATCTT 
CCTGTGATAT TATCAATTGA GAATCATTGT TCCCTACAAC AACAGGCGAA AATGGCACAG 
ATGTTCAAAA CAGTTCTTGG AGATTTACTA GTATCAAACT TCCTTTTTGA AGCTGATTTC 
TCGGACTCTC CACGTCTTCC ATGCCCTCTC CAAATGAAAA ATAAGATCTT GATAAAAAAT 
AAGAAAATGA TAGTCGACCC ACCAACTCCA TTGCCAATGA TTGAACGAGG AGCTGTGCAG 
CGTGGTGAAA CTCAATTAAA TCTTCACAGA AAACAGAGTA AAAACTCCTA CGAAAGCAGT 
ACGGTAGATG AAGTGGAAGA TGACGACCTA GATGAGTTTC TTGACGATGA AGAAAATGAG 
GAGGATGACC AAGAAGAGGT TCAAGTCAGA AGTGAAAAAG AAGACAGTCC GAAGACATCA 
AAACGTGCAG AGAAATCGGC TAGAAACATC AAACAGCAAG ATTCGTTATG TAGCGATCAT 
TCGGTAGAGC AGGCAAAACC TTCCACAAGC AAGACCACAT CCAAAACCAA CGATAGAAAG 
ACTGAAGATG AAGTACTTTA CGCTCAACTT GCTCAAAATG CCATCCGAAA CCAACAACCA 
AGAAAGAACA ATACTGGAGT CCAAATAGCG CCAGAGTTGT CAGATATTGT CATTTACATG 
CAAGCGACAA AATTCAAAGG ATTTCCCCCA GTCGATGGCA TTCAATCACC AAGGATAATG 
GAGGAAGGGC CAGCTTCTGC AAGTCTTTCA TTTTCATCAC GGGCTCGTAC GCCATCAAAT 
CTGCTGAATA CACCAGCCCC TCCACGACGA CAGCGAAGTA GCACTCAGTT GAGTCAAGAA 
CTGGCTGCAG AATTTTTGGG ATCAGTTAGG GCAAATGCTA CAGCTACATG CTACCAGGTT 
ACATCTCTTA ATGAAAACGC TGCAAAGAAA CTGATGAAAA GACATCCAGC AAAATGCGTA 
TCTTATACTC GGGACCATCT GATTCGTACG TATCCAAGTG CCAAGCACTA TGACTCGTCC 
AACTTTAACC CCATCAACTG CTGGGCTCAT GGAATGCAAA TGGTTGCTTT AAATTTTCAA 
ACTCCTGATG TTATAATGGC AGTTAACCAA GCGATGTTCG AGCAATCTGG AAACTGTGGA 
TATCAACTGA AACCACGTTG TCTTTGGGAT GAATCTCATC TTTTGTACAA CAAATTTTTG 
CCTCTCTCCA AAGACATTGC CGGCCATTCT GCTTTGTTAC TAAACTTAAC TATTATATCC 
GGACAGCACG TGTATCCAAA CACCCATTAC GCCAGTTTAT ATGTCGAAAT TGAAGTCATC 
GGTATTCACA ACGACTGTGT TCGTGAGAAA TCAAAAGTGG TCCAGAGAAA TTCTGTGAAT 
CCAATCTGGA ACCACACCAC TCAACTTCGA ATAGCTTGTG TTGACTTAGC ATTCCTTCGC 
ATAGCAGTAT GTGATAGTGG GCAAAATGGA AGAGTCGTTG CTCACCGAGT TGTTCCTGTC 
AAATGCATTC GTCCGGGTTT CCGACATCTC CCACTCAGAA CTCCAACTAA TCTTCCGATC 
GACAACGCTA TGATATTTTT AAGAACAAGA TTCGAGCAAG AAGAACACAT CTACCTCCAC 
GACGATGATT CAAACACCTA TTGTAATCTA GAACATACGC TGGCATACCG CACAGACTTA 
ACACCCAATT TATCTCCAAC GCCAATTTTG AAGAAACAGA TCTTTGTTCT TCGAATTACA 
GGCGCGTTTG CTGATGAAAC AGCAATTACA GTGCACAGCG AGTCAGGAAG TACAGTGAAG 
ACGGTGATGC AACAGGCGCT GCTTAACGCT GGAAAGAATG CGGATCAGGT TGAAGAGTAT 
GTACTCATCG AAGAATCACT GCCGGCACCT TCTGGGGAAG ATCCAATTGA GCAGAGAGTA 
CTTCCTCTGA ACGAGCCTAT CATGGACGCC GTGGCCTGCT GGAACGGTTC AATGCGTCGT 
TTTGTGTTAC GAAAAAAAGG CAGTGACCCA TCATCTAGAG CTTGGATCAC TTCAATTATA 
AAATCCGGAA CAAGTGGAAG CTCCACGTCA GTCTCACCAA GTCCGTTGAC CAAAGATGGA 
CACGTAAAGA GTGCATCAAG CAATCAACTT CATGGACGAT CATTGGACAC TGACGCTTTT 
GGAGAGCACC TTGAAGTGAC AGAAGGGTTG AACCCTCGTG CAAGATCAAT GGGCGACACA 
TTTCTGGTAT GTGTTCACAA TGTTAGTGAA GATCAGCCTT ATGCAATCCT ACGTGCTGGA 
ATCCACAGCA CTGCCGCCGA CATTATTCGT CAGGTGTTTG TTAAAGCTCG CCGTTCAAAT 
GTAGACGATT CCGAGTTTGT TCTTGTTGAA GAAACCTGCG ATGATCCAAA ACTCAATCAA 
GGCCAAATGA CTCCAAAGTA TCCAAACAAC CGCACCACAA GCCGAGTGTT AGGACAAAAC 
GAAAATGTTT GGAAAGCACA ATCGAGGTGG AAGTCAATGG GCAGATTTGT ACTTGAAAAC 
CGAAAGGACA CAGTGCACGC AACCCTCGAA AAAGTGAGAT CATTTATATC AAAACTGGAA 
GCTGCAAAAG CAAGCATGGA TCCCAGACAC GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001359720.1
CDS	1..10656
Translation	MSLRQASHKFPSVDSMPTSRDSSMETTDRYPFGYTTELARTEPSASTAKRLRQRLQRQQSGQSGLSGRRYTGESSECSSLVGTPTDTGRSFTFGAAQRVMRERDDRKPSVTQNLSEIMLEAIHYEDVNLVERLLTAHSSKQPMSTSPSIGSTNTLTDLAQRRHGAGAHRRSNASSTVSTHSGGRSTCAMMNTLHMAVAHKQRDIVELLLKNGYDPNTPASCHCKGNCTATGNIPLTSIIPRTHSMTPELCSTCSQLRVVSIVDQTPLGVAVRSQSSELIALLIAYGGDVNLGDEDGNTPLMLAVRESPLSWPCLHTLIFFGAQIEQKNMRGICPLDLAPELKKLQQTCVEELFKNACSGDCEPPSRSTPGPSAKNWNRLQVDNVSQGERSLSKAPLSPKPSAAASVSTCSMLETSSAKECARRKSLVSLQLHRKSRNVKEYPVIDCVSWEQAWELLKKMACNPECLDNIVNSLSKFATQMENTPSSVDRDQFDAHMGGLLHKMIHTAIEEYESSTPTYKKQKKLHLTWLLSQIATFCFTFLQKSGTSRQFSALNTLNKIIDAGLVHDLFAFNDVVFHSSRILNRCHAFDSDEPPTPSGESFNSNADQNSTVDHVFIYGAGYKDQSLLPSPTVAPTRHKSDLMNSFSEMDPSQVIVALHNAITMQNREVGSRSVCSPAHRWRQCCAHCTQILVARLLLFLTHAKKFRSRLSERHQLRSLVALLEPTLEPQLLCLLLQVLALIALDPSTHTTLIDIQIDDVLIQMLLPADDWYYTNHSTKYGTFVKYHAARVLVYVGMGDRVGSRVNLFNMMTFEPSAKSGSQQNEDDYICETCATPRSRSTFSRSAVSVEGVLLKVLAEVAELVRQSQHLSASEPITEESPSAGSPPAVLPNQAEQKSINEKNAEKLTQLGCQILISLEQLEAHLCKLGLVLDSVLLLRLLLHKLSWDLGLVTKKRVAVVDHVYKPITDPRAHSSCSLGNNRREDSKRDKNYLRVDQGSRASKRVHIRRSSSVEIIRPKRLSSGAKDIKNRERRKRLGTDTSSGSNRSKKTNSSSSSVQKHLPKYIQSLFRSRMGTDPCKRTSRSESTLPHRDSNESNTSGSDAVLEFTRKLQNYPLTRREAQRLTYRSNGQQDSNDSKMGPRTLGYVYLPELEIQGASPPRSPGPLTGNSLEEGTTMLLDTRRPSSPQAIPGLPLIEIRRPSALSQFEFGYFVNSPELSGSEVSDCAPLLLSGQIGGSRKSSDETSLGGWSSRASSVMSQRSSRSSVGLRLSTFSGGTSIASDNSGPFLFSFVLRKRASTIGTRIPIPRRALSRSSGDSLRVPDRESPLHLASMTEMNPDFQCVRQLILNLLNVYTKRNDNVVSTMKECADVLRQILNSPQHPTVKNWCAEIIHVVSTHVEEEEPTTAENNEQINDEYLEFQDQVISGSLPCPKEEAAYLASIQLSVEEQWPSNKRTQTIRRHLLKGQFGRIRDLAQKIMVTPWEVDQNLYCTPPRFPNESANASRAQSVVEEIQHRSRTPTLLRCITNTDGLMSEEMQAQCLPVDLRGDRRTIKLVKERKRKLFHSQVYESEIGMKKLYIQTAKKLAAFGCKVFQVKELLHGRTLRKTLRLLCLSSAQLCLLDGSTKLVLKRQHASTLQQWRVGGGVSKHQLLLEFRGTKWQLIAPSYNALKSISMTLWEIMQNSASNSIQRSLNQSMHRSHTEATVSTSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKVRSFISKLEAAKASMDPRHE

Target ORF information:

RefSeq Version	NM_001373494.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 (plc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373494.1

ORF Insert Sequence:

ATGTCCTTAC GCCAAGCATC ACACAAATTT CCATCGGTGG ACTCAATGCC AACATCACGG 
GATTCATCGA TGGAAACAAC CGACCGGTAC CCGTTTGGGT ATACGACCGA ATTGGCACGT 
ACCGAACCAT CTGCATCAAC TGCCAAACGT CTTCGACAAA GGTTACAGCG ACAGCAAAGT 
GGTCAATCAG GATTATCCGG TCGACGGTAT ACTGGAGAAA GCAGCGAGTG CTCATCACTT 
GTCGGAACAC CCACAGACAC TGGCAGATCA TTTACATTTG GAGCAGCACA AAGAGTCATG 
CGGGAACGGG ATGACCGCAA ACCATCAGTT ACCCAGAACT TGTCAGAAAT AATGCTCGAG 
GCAATACATT ATGAAGATGT GAACTTGGTG GAAAGGTTGC TCACTGCGCA TAGTTCAAAA 
CAACCAATGT CAACATCTCC GTCAATTGGT AGCACAAACA CATTGACGGA TCTTGCTCAA 
CGTAGACACG GTGCGGGGGC ACACCGACGT TCCAATGCCA GTAGTACGGT GTCCACACAT 
TCCGGAGGAA GATCCACGTG TGCTATGATG AACACGTTGC ACATGGCTGT GGCTCATAAA 
CAACGAGATA TAGTGGAACT TTTGCTTAAA AACGGCTACG ATCCCAATAC TCCCGCATCG 
TGTCACTGTA AGGGAAATTG CACGGCGACT GGGAATATTC CACTGACTTC GATCATTCCG 
AGAACTCATT CAATGACGCC AGAATTGTGT TCGACGTGCT CTCAACTGAG GGTTGTGTCA 
ATCGTGGATC AAACACCGCT TGGTGTGGCA GTCAGATCAC AGTCCAGCGA ATTGATAGCA 
TTGTTGATTG CCTACGGAGG AGATGTTAAT TTAGGTGATG AAGATGGCAA CACGCCGTTG 
ATGCTAGCCG TACGAGAATC ACCTCTGTCT TGGCCTTGCC TGCACACGCT CATTTTCTTT 
GGAGCTCAAA TTGAACAAAA GAACATGCGC GGAATATGCC CTCTTGATCT TGCTCCAGAG 
CTGAAGAAAC TTCAACAAAC TTGCGTTGAA GAGCTTTTCA AAAATGCGTG CTCGGGTGAT 
TGTGAACCAC CATCTCGATC TACACCGGGA CCATCAGCCA AAAACTGGAA TAGACTACAA 
GTTGACAATG TTTCCCAAGG AGAACGATCA CTGTCAAAAG CTCCATTGAG CCCAAAACCG 
TCTGCTGCAG CAAGTGTCTC AACGTGTAGC ATGCTGGAAA CCTCATCAGC AAAAGAATGT 
GCACGACGAA AATCATTGGT GTCGCTTCAA CTTCACAGAA AAAGCCGAAA CGTGAAAGAA 
TATCCTGTAA TTGACTGCGT CAGTTGGGAA CAAGCATGGG AATTGCTGAA GAAGATGGCA 
TGTAATCCAG AATGTCTAGA TAACATCGTG AATTCATTGT CAAAATTTGC AACCCAAATG 
GAGAACACCC CGTCAAGCGT TGATCGTGAT CAATTCGATG CACACATGGG TGGCCTACTA 
CATAAAATGA TTCATACAGC GATTGAGGAG TACGAGTCTT CGACACCAAC CTACAAAAAG 
CAGAAGAAAC TCCATCTCAC CTGGCTGCTC AGCCAAATTG CCACGTTTTG TTTTACATTT 
CTGCAGAAAA GTGGAACGAG CCGACAATTC TCAGCACTGA ACACGTTGAA TAAAATTATT 
GATGCTGGTT TAGTACATGA TTTGTTCGCA TTCAACGACG TTGTCTTTCA TTCTTCAAGA 
ATTTTGAACC GATGCCATGC ATTTGATTCC GACGAACCAC CAACTCCATC TGGCGAATCT 
TTCAACTCAA ATGCAGACCA AAACTCAACT GTCGATCATG TTTTCATTTA CGGAGCTGGC 
TATAAAGACC AATCGTTATT ACCTTCTCCT ACGGTGGCAC CAACTCGACA CAAGTCCGAC 
TTAATGAATT CTTTCAGCGA AATGGATCCA AGCCAGGTCA TTGTGGCCTT GCACAACGCG 
ATAACAATGC AAAACCGGGA AGTAGGGTCA AGATCTGTTT GTAGTCCGGC TCATCGATGG 
AGGCAGTGTT GTGCTCATTG TACACAGATA TTGGTGGCCA GATTATTGCT ATTCTTAACA 
CACGCAAAAA AATTTCGAAG TCGGTTGTCT GAACGACATC AGCTCAGAAG CTTGGTAGCT 
TTGTTAGAAC CAACTTTGGA ACCTCAACTC TTATGTCTTT TACTTCAAGT TCTAGCTCTT 
ATAGCGTTAG ATCCATCTAC CCACACAACT TTGATAGACA TTCAAATTGA CGACGTCTTG 
ATTCAAATGC TACTCCCAGC AGATGATTGG TACTACACTA ATCACAGCAC TAAATATGGA 
ACTTTTGTAA AGTACCATGC TGCTAGAGTT TTAGTGTACG TTGGAATGGG TGATCGAGTA 
GGAAGTCGAG TCAATCTGTT CAACATGATG ACGTTTGAGC CATCTGCAAA AAGCGGGAGT 
CAACAAAACG AGGACGACTA CATTTGTGAG ACATGTGCCA CACCGCGCAG CCGAAGTACA 
TTTTCAAGAT CAGCAGTTTC AGTGGAAGGT GTTCTGTTAA AAGTGTTGGC AGAAGTTGCT 
GAGCTTGTAC GTCAGAGTCA ACACCTATCA GCAAGTGAAC CAATAACTGA AGAATCGCCC 
AGTGCAGGAT CTCCACCCGC TGTCCTACCA AATCAGGCTG AACAGAAAAG TATTAACGAA 
AAAAATGCAG AGAAACTTAC ACAACTTGGG TGCCAAATTT TGATCTCATT GGAACAACTT 
GAAGCTCATT TATGCAAACT TGGACTGGTA CTTGATTCGG TTCTACTTCT CCGTCTACTT 
CTTCATAAGT TGTCATGGGA TCTTGGATTA GTCACAAAAA AGAGGGTTGC TGTGGTGGAT 
CATGTTTACA AACCAATCAC GGATCCGAGA GCTCATTCTA GCTGCAGTCT TGGAAACAAC 
AGGCGAGAAG ATAGCAAACG GGACAAAAAT TACTTGCGTG TTGATCAGGG ATCTCGTGCG 
TCAAAGAGAG TGCATATCAG ACGATCTAGT TCAGTAGAGA TTATCAGGCC AAAACGACTC 
AGTTCCGGAG CAAAAGATAT CAAAAATCGA GAACGGAGGA AAAGGCTGGG AACAGATACA 
AGTAGCGGGT CGAATCGTTC CAAGAAAACC AACTCCTCGT CTTCATCTGT TCAGAAACAC 
TTGCCAAAAT ACATTCAATC ATTATTCCGA AGTCGGATGG GAACTGATCC ATGCAAGAGG 
ACATCGAGAA GTGAATCCAC TTTACCGCAC AGAGACTCTA ATGAGAGTAA TACAAGTGGA 
AGTGATGCTG TTCTTGAGTT CACAAGAAAA CTTCAAAATT ATCCGTTGAC GAGACGCGAA 
GCGCAAAGAT TGACGTATCG AAGCAATGGG CAACAAGATT CAAATGACTC GAAGATGGGA 
CCACGAACTT TAGGATACGT TTACCTCCCA GAACTAGAAA TTCAAGGTGC AAGTCCGCCA 
AGATCACCGG GACCTCTAAC TGGAAACTCG TTAGAAGAAG GAACCACAAT GCTTTTGGAC 
ACAAGACGGC CGAGCAGCCC ACAAGCAATC CCCGGCCTTC CTTTAATTGA AATTCGTCGG 
CCCTCAGCTC TCTCTCAATT TGAATTTGGA TACTTTGTAA ACTCACCAGA ATTATCAGGA 
AGTGAAGTAT CTGATTGTGC CCCATTACTT CTCAGTGGTC AAATTGGTGG AAGCCGAAAA 
AGCTCCGACG AAACATCATT GGGAGGATGG TCCAGCCGTG CTTCATCGGT AATGAGTCAA 
AGGTCGTCCA GATCCAGTGT TGGCTTGAGG TTATCAACAT TTTCTGGAGG GACGAGTATT 
GCTTCGGATA ACTCTGGGCC ATTTTTGTTC AGTTTTGTTT TGAGAAAACG TGCATCTACT 
ATAGGAACTA GAATACCAAT ACCTAGGAGA GCTTTGTCAA GATCTTCAGG AGACAGTCTG 
AGAGTACCTG ATAGAGAAAG TCCACTTCAT TTAGCAAGTA TGACAGAGAT GAATCCGGAT 
TTTCAATGTG TTCGACAGCT CATCTTGAAC CTTTTGAATG TTTACACGAA AAGAAATGAC 
AATGTGGTTT CTACAATGAA GGAATGTGCG GATGTTCTTC GTCAAATCCT AAACTCTCCT 
CAACATCCAA CTGTCAAGAA TTGGTGTGCC GAAATAATTC ATGTAGTCAG TACTCATGTA 
GAAGAAGAGG AACCAACGAC GGCAGAAAAT AATGAACAAA TTAATGACGA GTATCTGGAA 
TTTCAAGACC AAGTGATATC AGGAAGTTTA CCATGCCCGA AGGAAGAGGC TGCATACCTT 
GCAAGTATTC AGTTATCAGT TGAAGAGCAG TGGCCGAGTA ATAAAAGGAC CCAGACAATT 
AGAAGACATT TATTGAAAGG ACAGTTTGGT CGAATCCGGG ATCTCGCCCA AAAGATCATG 
GTGACTCCAT GGGAAGTTGA TCAAAACTTG TACTGCACAC CACCCAGATT TCCCAATGAA 
TCGGCAAATG CTAGTAGAGC ACAATCAGTG GTTGAGGAAA TTCAGCACAG ATCAAGAACT 
CCAACTCTTC TGCGATGTAT CACAAACACA GACGGCTTAA TGTCAGAAGA GATGCAAGCT 
CAGTGCCTTC CCGTCGATCT ACGTGGTGAT CGTAGAACTA TCAAGCTGGT CAAGGAGAGG 
AAGCGAAAAT TGTTCCATAG TCAAGTCTAT GAAAGTGAAA TTGGCATGAA GAAATTGTAC 
ATTCAGACCG CCAAAAAACT AGCCGCATTT GGTTGTAAGG TTTTTCAAGT TAAAGAGTTG 
CTTCACGGAC GAACACTCAG AAAAACTCTT CGACTGCTTT GCCTTTCTTC TGCTCAATTG 
TGTCTTCTCG ACGGATCAAC AAAACTTGTG CTTAAACGGC AGCACGCATC GACGTTACAA 
CAGTGGAGAG TAGGTGGTGG AGTTAGTAAG CATCAACTAT TGCTCGAATT CCGTGGAACC 
AAGTGGCAAC TTATTGCACC GTCGTATAAT GCACTGAAAT CGATATCGAT GACATTATGG 
GAGATTATGC AAAATTCTGC TAGCAATTCA ATCCAGCGGA GTCTCAACCA GTCAATGCAT 
CGTTCGCACA CCGAGGCGAC TGTCTCAACA TCACGTAGTG ACTCAACCTC TTCAACGCAA 
TCAGCCACAG CTGGATTCAT TCTCAATGAA GAACCAATCA CACTGTTCCG ACTTGAACTC 
GAACGACTTC AATATATTCT TCATTTCCCT GAAGAAGTTG CATTCCAACT ATCTTCTACT 
GAGTATCAGC TTTTCTACTC AATACAACCC ATGGATTATG TTCGTTACGT CAGTTGCGAT 
CTCACATCTG TTCCAGTGTC AGAAAATCCA AGTCCAGTGC GAAATCTTGT GAAAAGGCTA 
TCAGAGGTAT CATCCTGGAT AACACATGTT ATTGTCAGTC AGCCAACACA TGACGATCGA 
AAGGTTGCTC TTACTGCAAT TTTGAGAATT GTGGAAACTT GCTGGAATAT TGGAAACTTT 
AATGCAGCTG TTGAGGTTCT TATGGGTTTA AAGAGTGAAA AACTCAGACC ATTCTGGCTT 
TCTTTGCGAC AAGAGGAAAA GTCTCAGTTT GATTCGCTTT GCGAAACCTT GCTTCCTGCA 
AACCAAGCTC TCCCATCTCA AGCTTACATC AACGCGGTCC AACGAGCACT GAGAATGCCA 
CAATCTCGTG TGATTCCATT TTTTGGAATA TTTCTTCGTG ATCTCTACGC AATTGTGAAT 
GACTTGCCGA ATATCGTTGT GATTGGACAA GAAGGTGAAA CCCAAAAGCT GGAATTCATG 
AGCGATCCCA ATGGCGAAGA TCATTTTTCG TCGAGAATTG GAGTGGGTGG GCTGTTGAAT 
GCTGATAAAA TCAACTTGGT GGCAATTGTT TTGGACAACT TGGAATTATT CCATCGTCAC 
AGCAGGACAA TGATCAAATT GTTAGAAGAG CAAGCAGTCC CACCAATTCA AATTCCTCAG 
AACGAGCGGG AGCAAAAAGA AAAGGAAGCA AAAACATATG AACCAGTACA AGTGGTTCGC 
GGCTCTTCTC ATGGAGTGGC TCTCATCCCT CTTGATACCC TAACATTCGA TCTTGATGTT 
ATCCAACGAC TCCAGCACGG AACAACAGTG ATCCATTACG AACCTGATTC GGGAAGAAGC 
AATTTGTGTC TGCTGCGGTT AGACCCTAGT TGCGGTCAAA TAAACTGGCA TAAGATTTCA 
TATTCAGTTA ACAAAGATCC CAAAGAAAAG GATGTGCTCG CCAAAGTATC CGTTTCAAAT 
CTGCAGCCGC TAGACTCTGG CCGAGGGGCT CCGTCACCAA TGCCATCTGG AAGAACACCA 
GGAACTGGAG GAGTTGGAGT AGAGGAGGGA GAACTCAAAT TAAGTGTTGT GAAAGGAGTC 
GAACTAGTTG ATAGTTATGA TATTGATATT GAGGCAATTT ACCGTCGTCA TTCTATGGAA 
GAGATGTCAG TTCCTGTTAG CTGTTGGAAA GTTAGTCATG GTCAGTTGTT ATCGGATAAT 
GAGTTCATCT ACTTTTTGGC CCCTCAACAA ATTGCTCAAT TTTGGACCAA CGGGTTGCAA 
TCTGTAGTTA AAAGTCTCCA AGGACAGCAA AGGTACCCTG ATCGGCGAAT GTTATGGATC 
AAAAATGTCT ACTTATCACT TTACGAGATA ACGGGCGAAT CAAACTGTGG ACCACGGCCA 
TTTGAAGCAC TTCAGGCATT TGGGCTGAGT CAAACAAATA CTAACGCAAC ACGACCCAAT 
GACTCGTCGC TGTCCAGTGA GCCAGGAGGA GCTAAAAGCA GACTGAAGAA CCTTAAAAAT 
GCTATGCAAA AGAAGCTTCG TGGTGCATCG CGCGAAGGAA GCAGATCTCA GTCACCTCAA 
CCTCATTCTC CTCTTGTCCG ACCACCATCA ATAAAATCGC AAATATCGTC ACAATCTGGT 
CCGCCGGGTC CGAACTCTCC AGGCTATTTG CTGAAACCAC GTGGAGAACC TGCCAATAGT 
GATGCAGGAG ACATTGATAG CATTTATACA CCTCGATCAA GAACTCCAAC AAGCAGCTCC 
TATGGAGGGC GATCCGTTGG AGGGAGATCA TGTAAATCGT GGAGATCAAG AGGAGGAGAG 
ACACCAAATT CGGGTTCCAT AAGCAGCTCG GGTCAGATGT CAATTCAAGT GTCTGGTCTG 
AGTGGACCAT CGGGAAAAGA GTTTCAGGAA AAACCATTAA CACTTGTAGA GTTCGCTGAA 
CTTTTTCGAT TGTTCAATAC AAGAATGAGA AAAGATTTGA GAGATGTGTT CAATGATGTC 
CTGTCTACGG CAACTACTCC TCAGCATTGT CCTAAAAGAG AACGTGATCG CCACAGTCCG 
AGAATGCAGT CAAGGCTGGC GAGTGTCTCC AACTCGTACA ATGCAGATTT TCTGTCTAAC 
GATTTCTTGA CTCGGAATAC GGCAGTAACG TCCCATCATA TCAGTGAAAA GCAAAACAAA 
ATCTACAATG CATTGGCACT TGCGAGTGTT AATAGCATGG GCGGACTCAT GGACACTTCC 
CGGTCGTCGA TGCTGACGCC GCAAATGCTG CGCGCTTTTG TCAACACTCA TCAGATGGAG 
CAGATTGACG AGCAAACGGC GATTAAGCTT ATTCAGGATC ATGAACCTGA TGGTATCTGT 
CGACAAAAGA ATCAAATGTC ATTCGAAGGA TTCACTAGAT TCCTTTGCGA TCCTGTCAAC 
TTTGCATTCG TACCGGAAAC AATTGAACCA GATGAAGAGG ACCTACGGTA CCCGTTGTCT 
CATTATTACA TCAATTCAAG TCACAACACC TATTTGACGG GACATCAACT CAAGGGACCC 
AGTAGCTCGG AGATGTACAG ACAGGTTCTC CTCACCGGGT GTCGGTGTGT CGAGCTTGAT 
TGTTGGGATG GAGATGACGG ATTGCCATTG ATTTATCATG GACACACATT GGTTAGCAAG 
ATTGGGTTTA GACAGGTGGT CGAAATTATC AAAAAATCTG CATTCATAAC ATCGGATCTT 
CCTGTGATAT TATCAATTGA GAATCATTGT TCCCTACAAC AACAGGCGAA AATGGCACAG 
ATGTTCAAAA CAGTTCTTGG AGATTTACTA GTATCAAACT TCCTTTTTGA AGCTGATTTC 
TCGGACTCTC CACGTCTTCC ATGCCCTCTC CAAATGAAAA ATAAGATCTT GATAAAAAAT 
AAGAAAATGA TAGTCGACCC ACCAACTCCA TTGCCAATGA TTGAACGAGG AGCTGTGCAG 
CGTGGTGAAA CTCAATTAAA TCTTCACAGA AAACAGAGTA AAAACTCCTA CGAAAGCAGT 
ACGGTAGATG AAGTGGAAGA TGACGACCTA GATGAGTTTC TTGACGATGA AGAAAATGAG 
GAGGATGACC AAGAAGAGGT TCAAGTCAGA AGTGAAAAAG AAGACAGTCC GAAGACATCA 
AAACGTGCAG AGAAATCGGC TAGAAACATC AAACAGCAAG ATTCGTTATG TAGCGATCAT 
TCGGTAGAGC AGGCAAAACC TTCCACAAGC AAGACCACAT CCAAAACCAA CGATAGAAAG 
ACTGAAGATG AAGTACTTTA CGCTCAACTT GCTCAAAATG CCATCCGAAA CCAACAACCA 
AGAAAGAACA ATACTGGAGT CCAAATAGCG CCAGAGTTGT CAGATATTGT CATTTACATG 
CAAGCGACAA AATTCAAAGG ATTTCCCCCA GTCGATGGCA TTCAATCACC AAGGATAATG 
GAGGAAGGGC CAGCTTCTGC AAGTCTTTCA TTTTCATCAC GGGCTCGTAC GCCATCAAAT 
CTGCTGAATA CACCAGCCCC TCCACGACGA CAGCGAAGTA GCACTCAGTT GAGTCAAGAA 
CTGGCTGCAG AATTTTTGGG ATCAGTTAGG GCAAATGCTA CAGCTACATG CTACCAGGTT 
ACATCTCTTA ATGAAAACGC TGCAAAGAAA CTGATGAAAA GACATCCAGC AAAATGCGTA 
TCTTATACTC GGGACCATCT GATTCGTACG TATCCAAGTG CCAAGCACTA TGACTCGTCC 
AACTTTAACC CCATCAACTG CTGGGCTCAT GGAATGCAAA TGGTTGCTTT AAATTTTCAA 
ACTCCTGATG TTATAATGGC AGTTAACCAA GCGATGTTCG AGCAATCTGG AAACTGTGGA 
TATCAACTGA AACCACGTTG TCTTTGGGAT GAATCTCATC TTTTGTACAA CAAATTTTTG 
CCTCTCTCCA AAGACATTGC CGGCCATTCT GCTTTGTTAC TAAACTTAAC TATTATATCC 
GGACAGCACG TGTATCCAAA CACCCATTAC GCCAGTTTAT ATGTCGAAAT TGAAGTCATC 
GGTATTCACA ACGACTGTGT TCGTGAGAAA TCAAAAGTGG TCCAGAGAAA TTCTGTGAAT 
CCAATCTGGA ACCACACCAC TCAACTTCGA ATAGCTTGTG TTGACTTAGC ATTCCTTCGC 
ATAGCAGTAT GTGATAGTGG GCAAAATGGA AGAGTCGTTG CTCACCGAGT TGTTCCTGTC 
AAATGCATTC GTCCGGGTTT CCGACATCTC CCACTCAGAA CTCCAACTAA TCTTCCGATC 
GACAACGCTA TGATATTTTT AAGAACAAGA TTCGAGCAAG AAGAACACAT CTACCTCCAC 
GACGATGATT CAAACACCTA TTGTAATCTA GAACATACGC TGGCATACCG CACAGACTTA 
ACACCCAATT TATCTCCAAC GCCAATTTTG AAGAAACAGA TCTTTGTTCT TCGAATTACA 
GGCGCGTTTG CTGATGAAAC AGCAATTACA GTGCACAGCG AGTCAGGAAG TACAGTGAAG 
ACGGTGATGC AACAGGCGCT GCTTAACGCT GGAAAGAATG CGGATCAGGT TGAAGAGTAT 
GTACTCATCG AAGAATCACT GCCGGCACCT TCTGGGGAAG ATCCAATTGA GCAGAGAGTA 
CTTCCTCTGA ACGAGCCTAT CATGGACGCC GTGGCCTGCT GGAACGGTTC AATGCGTCGT 
TTTGTGTTAC GAAAAAAAGG CAGTGACCCA TCATCTAGAG CTTGGATCAC TTCAATTATA 
AAATCCGGAA CAAGTGGAAG CTCCACGTCA GTCTCACCAA GTCCGTTGAC CAAAGATGGA 
CACGTAAAGA GTGCATCAAG CAATCAACTT CATGGACGAT CATTGGACAC TGACGCTTTT 
GGAGAGCACC TTGAAGTGAC AGAAGGGTTG AACCCTCGTG CAAGATCAAT GGGCGACACA 
TTTCTGGTAT GTGTTCACAA TGTTAGTGAA GATCAGCCTT ATGCAATCCT ACGTGCTGGA 
ATCCACAGCA CTGCCGCCGA CATTATTCGT CAGGTGTTTG TTAAAGCTCG CCGTTCAAAT 
GTAGACGATT CCGAGTTTGT TCTTGTTGAA GAAACCTGCG ATGATCCAAA ACTCAATCAA 
GGCCAAATGA CTCCAAAGTA TCCAAACAAC CGCACCACAA GCCGAGTGTT AGGACAAAAC 
GAAAATGTTT GGAAAGCACA ATCGAGGTGG AAGTCAATGG GCAGATTTGT ACTTGAAAAC 
CGAAAGGACA CAGTGCACGC AACCCTCGAA AAAGTGAGAT CATTTATATC AAAACTGGAA 
GCTGCAAAAG CAAGCATGGA TCCCAGACAC GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_001129669.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001123141.2 Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.

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CloneID	OCa851221
Clone ID Related Accession (Same CDS sequence)	NM_001129669.3
Accession Version	NM_001129669.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	PhosphoLipase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_001129669.2. COMPLETENESS: incomplete on the 5' end.

ATGTCCTTAC GCCAAGCATC ACACAAATTT CCATCGGTGG ACTCAATGCC AACATCACGG 
GATTCATCGA TGGAAACAAC CGACCGGTAC CCGTTTGGGT ATACGACCGA ATTGGCACGT 
ACCGAACCAT CTGCATCAAC TGCCAAACGT CTTCGACAAA GGTTACAGCG ACAGCAAAGT 
GGTCAATCAG GATTATCCGG TCGACGGTAT ACTGGAGAAA GCAGCGAGTG CTCATCACTT 
GTCGGAACAC CCACAGACAC TGGCAGATCA TTTACATTTG GAGCAGCACA AAGAGTCATG 
CGGGAACGGG ATGACCGCAA ACCATCAGTT ACCCAGAACT TGTCAGAAAT AATGCTCGAG 
GCAATACATT ATGAAGATGT GAACTTGGTG GAAAGGTTGC TCACTGCGCA TAGTTCAAAA 
CAACCAATGT CAACATCTCC GTCAATTGGT AGCACAAACA CATTGACGGA TCTTGCTCAA 
CGTAGACACG GTGCGGGGGC ACACCGACGT TCCAATGCCA GTAGTACGGT GTCCACACAT 
TCCGGAGGAA GATCCACGTG TGCTATGATG AACACGTTGC ACATGGCTGT GGCTCATAAA 
CAACGAGATA TAGTGGAACT TTTGCTTAAA AACGGCTACG ATCCCAATAC TCCCGCATCG 
TGTCACTGTA AGGGAAATTG CACGGCGACT GGGAATATTC CACTGACTTC GATCATTCCG 
AGAACTCATT CAATGACGCC AGAATTGTGT TCGACGTGCT CTCAACTGAG GGTTGTGTCA 
ATCGTGGATC AAACACCGCT TGGTGTGGCA GTCAGATCAC AGTCCAGCGA ATTGATAGCA 
TTGTTGATTG CCTACGGAGG AGATGTTAAT TTAGGTGATG AAGATGGCAA CACGCCGTTG 
ATGCTAGCCG TACGAGAATC ACCTCTGTCT TGGCCTTGCC TGCACACGCT CATTTTCTTT 
GGAGCTCAAA TTGAACAAAA GAACATGCGC GGAATATGCC CTCTTGATCT TGCTCCAGAG 
CTGAAGAAAC TTCAACAAAC TTGCGTTGAA GAGCTTTTCA AAAATGCGTG CTCGGGTGAT 
TGTGAACCAC CATCTCGATC TACACCGGGA CCATCAGCCA AAAACTGGAA TAGACTACAA 
GTTGACAATG ATCCTGTAAT TGACTGCGTC AGTTGGGAAC AAGCATGGGA ATTGCTGAAG 
AAGATGGCAT GTAATCCAGA ATGTCTAGAT AACATCGTGA ATTCATTGTC AAAATTTGCA 
ACCCAAATGG AGAACACCCC GTCAAGCGTT GATCGTGATC AATTCGATGC ACACATGGGT 
GGCCTACTAC ATAAAATGAT TCATACAGCG ATTGAGGAGT ACGAGTCTTC GACACCAACC 
TACAAAAAGC AGAAGAAACT CCATCTCACC TGGCTGCTCA GCCAAATTGC CACGTTTTGT 
TTTACATTTC TGCAGAAAAG TGGAACGAGC CGACAATTCT CAGCACTGAA CACGTTGAAT 
AAAATTATTG ATGCTGGTTT AGTACATGAT TTGTTCGCAT TCAACGACGT TGTCTTTCAT 
TCTTCAAGAA TTTTGAACCG ATGCCATGCA TTTGATTCCG ACGAACCACC AACTCCATCT 
GGCGAATCTT TCAACTCAAA TGCAGACCAA AACTCAACTG TCGATCATGT TTTCATTTAC 
GGAGCTGGCT ATAAAGACCA ATCGTTATTA CCTTCTCCTA CGGTGGCACC AACTCGACAC 
AAGTCCGACT TAATGAATTC TTTCAGCGAA ATGGATCCAA GCCAGGTCAT TGTGGCCTTG 
CACAACGCGA TAACAATGCA AAACCGGGAA GTAGGGTCAA GATCTGTTTG TAGTCCGGCT 
CATCGATGGA GGCAGTGTTG TGCTCATTGT ACACAGATAT TGGTGGCCAG ATTATTGCTA 
TTCTTAACAC ACGCAAAAAA ATTTCGAAGT CGGTTGTCTG AACGACATCA GCTCAGAAGC 
TTGGTAGCTT TGTTAGAACC AACTTTGGAA CCTCAACTCT TATGTCTTTT ACTTCAAGTT 
CTAGCTCTTA TAGCGTTAGA TCCATCTACC CACACAACTT TGATAGACAT TCAAATTGAC 
GACGTCTTGA TTCAAATGCT ACTCCCAGCA GATGATTGGT ACTACACTAA TCACAGCACT 
AAATATGGAA CTTTTGTAAA GTACCATGCT GCTAGAGTTT TAGTGTACGT TGGAATGGGT 
GATCGAGTAG GAAGTCGAGT CAATCTGTTC AACATGATGA CGTTTGAGCC ATCTGCAAAA 
AGCGGGAGTC AACAAAACGA GGACGACTAC ATTTGTGAGA CATGTGCCAC ACCGCGCAGC 
CGAAGTACAT TTTCAAGATC AGCAGTTTCA GTGGAAGGTG TTCTGTTAAA AGTGTTGGCA 
GAAGTTGCTG AGCTTGTACG TCAGAGTCAA CACCTATCAG CAAGTGAACC AATAACTGAA 
GAATCGCCCA GTGCAGGATC TCCACCCGCT GTCCTACCAA ATCAGGCTGA ACAGAAAAGT 
ATTAACGAAA AAAATGCAGA GAAACTTACA CAACTTGGGT GCCAAATTTT GATCTCATTG 
GAACAACTTG AAGCTCATTT ATGCAAACTT GGACTGGTAC TTGATTCGGT TCTACTTCTC 
CGTCTACTTC TTCATAAGTT GTCATGGGAT CTTGGATTAG TCACAAAAAA GAGGGTTGCT 
GTGGTGGATC ATGTTTACAA ACCAATCACG GATCCGAGAG CTCATTCTAG CTGCAGTCTT 
GGAAACAACA GGCGAGAAGA TAGCAAACGG GACAAAAATT ACTTGCGTGT TGATCAGGGA 
TCTCGTGCGT CAAAGAGAGT GCATATCAGA CGATCTAGTT CAGTAGAGAT TATCAGGCCA 
AAACGACTCA GTTCCGGAGC AAAAGATATC AAAAATCGAG AACGGAGGAA AAGGCTGGGA 
ACAGATACAA GTAGCGGGTC GAATCGTTCC AAGAAAACCA ACTCCTCGTC TTCATCTGTT 
CAGAAACACT TGCCAAAATA CATTCAATCA TTATTCCGAA GTCGGATGGG AACTGATCCA 
TGCAAGAGGA CATCGAGAAG TGAATCCACT TTACCGCACA GAGACTCTAA TGAGAGTAAT 
ACAAGTGGAA GTGATGCTGT TCTTGAGTTC ACAAGAAAAC TTCAAAATTA TCCGTTGACG 
AGACGCGAAG CGCAAAGATT GACGTATCGA AGCAATGGGC AACAAGATTC AAATGACTCG 
AAGATGGGAC CACGAACTTT AGGATACGTT TACCTCCCAG AACTAGAAAT TCAAGGTGCA 
AGTCCGCCAA GATCACCGGG ACCTCTAACT GGAAACTCGT TAGAAGAAGG AACCACAATG 
CTTTTGGACA CAAGACGGCC GAGCAGCCCA CAAGCAATCC CCGGCCTTCC TTTAATTGAA 
ATTCGTCGGC CCTCAGCTCT CTCTCAATTT GAATTTGGAT ACTTTGTAAA CTCACCAGAA 
TTATCAGGAA GTGAAGTATC TGATTGTGCC CCATTACTTC TCAGTGGTCA AATTGGTGGA 
AGCCGAAAAA GCTCCGACGA AACATCATTG GGAGGATGGT CCAGCCGTGC TTCATCGGTA 
ATGAGTCAAA GGTCGTCCAG ATCCAGTGTT GGCTTGAGGT TATCAACATT TTCTGGAGGG 
ACGAGTATTG CTTCGGATAA CTCTGGGCCA TTTTTGTTCA GTTTTGTTTT GAGAAAACGT 
GCATCTACTA TAGGAACTAG AATACCAATA CCTAGGAGAG CTTTGTCAAG ATCTTCAGGA 
GACAGTCTGA GAGTACCTGA TAGAGAAAGT CCACTTCATT TAGCAAGTAT GACAGAGATG 
AATCCGGATT TTCAATGTGT TCGACAGCTC ATCTTGAACC TTTTGAATGT TTACACGAAA 
AGAAATGACA ATGTGGTTTC TACAATGAAG GAATGTGCGG ATGTTCTTCG TCAAATCCTA 
AACTCTCCTC AACATCCAAC TGTCAAGAAT TGGTGTGCCG AAATAATTCA TGTAGTCAGT 
ACTCATGTAG AAGAAGAGGA ACCAACGACG GCAGAAAATA ATGAACAAAT TAATGACGAG 
TATCTGGAAT TTCAAGACCA AGTGATATCA GGAAGTTTAC CATGCCCGAA GGAAGAGGCT 
GCATACCTTG CAAGTATTCA GTTATCAGTT GAAGAGCAGT GGCCGAGTAA TAAAAGGACC 
CAGACAATTA GAAGACATTT ATTGAAAGGA CAGTTTGGTC GAATCCGGGA TCTCGCCCAA 
AAGATCATGG TGACTCCATG GGAAGTTGAT CAAAACTTGT ACTGCACACC ACCCAGATTT 
CCCAATGAAT CGGCAAATGC TAGTAGAGCA CAATCAGTGG TTGAGGAAAT TCAGCACAGA 
TCAAGAACTC CAACTCTTCT GCGATGTATC ACAAACACAG ACGGCTTAAT GTCAGAAGAG 
ATGCAAGCTC AGTGCCTTCC CGTCGATCTA CGTGGTGATC GTAGAACTAT CAAGCTGGTC 
AAGGAGAGGA AGCGAAAATT GTTCCATAGT CAAGTCTATG AAAGTGAAAT TGGCATGAAG 
AAATTGTACA TTCAGACCGC CAAAAAACTA GCCGCATTTG GTTGTAAGGT TTTTCAAGTT 
AAAGAGTTGC TTCACGGACG AACACTCAGA AAAACTCTTC GACTGCTTTG CCTTTCTTCT 
GCTCAATTGT GTCTTCTCGA CGGATCAACA AAACTTGTGC TTAAACGGCA GCACGCATCG 
ACGTTACAAC AGTGGAGAGT AGGTGGTGGA GTTAGTAAGC ATCAACTATT GCTCGAATTC 
CGTGGAACCA AGTGGCAACT TATTGCACCG TCGTATAATG CACTGAAATC GATATCGATG 
ACATTATGGG AGATTATGCA AAATTCTGCT AGCAATTCAA TCCAGCGGAG TCTCAACCAG 
TCAATGCATC GTTCGCACAC CGAGGCGACT GTCTCAACAT CACGTAGTGA CTCAACCTCT 
TCAACGCAAT CAGCCACAGC TGGATTCATT CTCAATGAAG AACCAATCAC ACTGTTCCGA 
CTTGAACTCG AACGACTTCA ATATATTCTT CATTTCCCTG AAGAAGTTGC ATTCCAACTA 
TCTTCTACTG AGTATCAGCT TTTCTACTCA ATACAACCCA TGGATTATGT TCGTTACGTC 
AGTTGCGATC TCACATCTGT TCCAGTGTCA GAAAATCCAA GTCCAGTGCG AAATCTTGTG 
AAAAGGCTAT CAGAGGTATC ATCCTGGATA ACACATGTTA TTGTCAGTCA GCCAACACAT 
GACGATCGAA AGGTTGCTCT TACTGCAATT TTGAGAATTG TGGAAACTTG CTGGAATATT 
GGAAACTTTA ATGCAGCTGT TGAGGTTCTT ATGGGTTTAA AGAGTGAAAA ACTCAGACCA 
TTCTGGCTTT CTTTGCGACA AGAGGAAAAG TCTCAGTTTG ATTCGCTTTG CGAAACCTTG 
CTTCCTGCAA ACCAAGCTCT CCCATCTCAA GCTTACATCA ACGCGGTCCA ACGAGCACTG 
AGAATGCCAC AATCTCGTGT GATTCCATTT TTTGGAATAT TTCTTCGTGA TCTCTACGCA 
ATTGTGAATG ACTTGCCGAA TATCGTTGTG ATTGGACAAG AAGGTGAAAC CCAAAAGCTG 
GAATTCATGA GCGATCCCAA TGGCGAAGAT CATTTTTCGT CGAGAATTGG AGTGGGTGGG 
CTGTTGAATG CTGATAAAAT CAACTTGGTG GCAATTGTTT TGGACAACTT GGAATTATTC 
CATCGTCACA GCAGGACAAT GATCAAATTG TTAGAAGAGC AAGCAGTCCC ACCAATTCAA 
ATTCCTCAGA ACGAGCGGGA GCAAAAAGAA AAGGAAGCAA AAACATATGA ACCAGTACAA 
GTGGTTCGCG GCTCTTCTCA TGGAGTGGCT CTCATCCCTC TTGATACCCT AACATTCGAT 
CTTGATGTTA TCCAACGACT CCAGCACGGA ACAACAGTGA TCCATTACGA ACCTGATTCG 
GGAAGAAGCA ATTTGTGTCT GCTGCGGTTA GACCCTAGTT GCGGTCAAAT AAACTGGCAT 
AAGATTTCAT ATTCAGTTAA CAAAGATCCC AAAGAAAAGG ATGTGCTCGC CAAAGTATCC 
GTTTCAAATC TGCAGCCGCT AGACTCTGGC CGAGGGGCTC CGTCACCAAT GCCATCTGGA 
AGAACACCAG GAACTGGAGG AGTTGGAGTA GAGGAGGGAG AACTCAAATT AAGTGTTGTG 
AAAGGAGTCG AACTAGTTGA TAGTTATGAT ATTGATATTG AGGCAATTTA CCGTCGTCAT 
TCTATGGAAG AGATGTCAGT TCCTGTTAGC TGTTGGAAAG TTAGTCATGG TCAGTTGTTA 
TCGGATAATG AGTTCATCTA CTTTTTGGCC CCTCAACAAA TTGCTCAATT TTGGACCAAC 
GGGTTGCAAT CTGTAGTTAA AAGTCTCCAA GGACAGCAAA GGTACCCTGA TCGGCGAATG 
TTATGGATCA AAAATGTCTA CTTATCACTT TACGAGATAA CGGGCGAATC AAACTGTGGA 
CCACGGCCAT TTGAAGCACT TCAGGCATTT GGGCTGAGTC AAACAAATAC TAACGCAACA 
CGACCCAATG ACTCGTCGCT GTCCAGTGAG CCAGGAGGAG CTAAAAGCAG ACTGAAGAAC 
CTTAAAAATG CTATGCAAAA GAAGCTTCGT GGTGCATCGC GCGAAGGAAG CAGATCTCAG 
TCACCTCAAC CTCATTCTCC TCTTGTCCGA CCACCATCAA TAAAATCGCA AATATCGTCA 
CAATCTGGTC CGCCGGGTCC GAACTCTCCA GGCTATTTGC TGAAACCACG TGGAGAACCT 
GCCAATAGTG ATGCAGGAGA CATTGATAGC ATTTATACAC CTCGATCAAG AACTCCAACA 
AGCAGCTCCT ATGGAGGGCG ATCCGTTGGA GGGAGATCAT GTAAATCGTG GAGATCAAGA 
GGAGGAGAGA CACCAAATTC GGGTTCCATA AGCAGCTCGG GTCAGATGTC AATTCAAGTG 
TCTGGTCTGA GTGGACCATC GGGAAAAGAG TTTCAGGAAA AACCATTAAC ACTTGTAGAG 
TTCGCTGAAC TTTTTCGATT GTTCAATACA AGAATGAGAA AAGATTTGAG AGATGTGTTC 
AATGATGTCC TGTCTACGGC AACTACTCCT CAGCATTGTC CTAAAAGAGA ACGTGATCGC 
CACAGTCCGA GAATGCAGTC AAGGCTGGCG AGTGTCTCCA ACTCGTACAA TGCAGATTTT 
CTGTCTAACG ATTTCTTGAC TCGGAATACG GCAGTAACGT CCCATCATAT CAGTGAAAAG 
CAAAACAAAA TCTACAATGC ATTGGCACTT GCGAGTGTTA ATAGCATGGG CGGACTCATG 
GACACTTCCC GGTCGTCGAT GCTGACGCCG CAAATGCTGC GCGCTTTTGT CAACACTCAT 
CAGATGGAGC AGATTGACGA GCAAACGGCG ATTAAGCTTA TTCAGGATCA TGAACCTGAT 
GGTATCTGTC GACAAAAGAA TCAAATGTCA TTCGAAGGAT TCACTAGATT CCTTTGCGAT 
CCTGTCAACT TTGCATTCGT ACCGGAAACA ATTGAACCAG ATGAAGAGGA CCTACGGTAC 
CCGTTGTCTC ATTATTACAT CAATTCAAGT CACAACACCT ATTTGACGGG ACATCAACTC 
AAGGGACCCA GTAGCTCGGA GATGTACAGA CAGGTTCTCC TCACCGGGTG TCGGTGTGTC 
GAGCTTGATT GTTGGGATGG AGATGACGGA TTGCCATTGA TTTATCATGG ACACACATTG 
GTTAGCAAGA TTGGGTTTAG ACAGGTGGTC GAAATTATCA AAAAATCTGC ATTCATAACA 
TCGGATCTTC CTGTGATATT ATCAATTGAG AATCATTGTT CCCTACAACA ACAGGCGAAA 
ATGGCACAGA TGTTCAAAAC AGTTCTTGGA GATTTACTAG TATCAAACTT CCTTTTTGAA 
GCTGATTTCT CGGACTCTCC ACGTCTTCCA TGCCCTCTCC AAATGAAAAA TAAGATCTTG 
ATAAAAAATA AGAAAATGAT AGTCGACCCA CCAACTCCAT TGCCAATGAT TGAACGAGGA 
GCTGTGCAGC GTGGTGAAAC TCAATTAAAT CTTCACAGAA AACAGAGTAA AAACTCCTAC 
GAAAGCAGTA CGGTAGATGA AGTGGAAGAT GACGACCTAG ATGAGTTTCT TGACGATGAA 
GAAAATGAGG AGGATGACCA AGAAGAGGTT CAAGTCAGAA GTGAAAAAGA AGACAGTCCG 
AAGACATCAA AACGTGCAGA GAAATCGGCT AGAAACATCA AACAGCAAGA TTCGTTATGT 
AGCGATCATT CGGTAGAGCA GGCAAAACCT TCCACAAGCA AGACCACATC CAAAACCAAC 
GATAGAAAGA CTGAAGATGA AGTACTTTAC GCTCAACTTG CTCAAAATGC CATCCGAAAC 
CAACAACCAA GAAAGAACAA TACTGGAGTC CAAATAGCGC CAGAGTTGTC AGATATTGTC 
ATTTACATGC AAGCGACAAA ATTCAAAGGA TTTCCCCCAG TCGATGGCAT TCAATCACCA 
AGGATAATGG AGGAAGGGCC AGCTTCTGCA AGTCTTTCAT TTTCATCACG GGCTCGTACG 
CCATCAAATC TGCTGAATAC ACCAGCCCCT CCACGACGAC AGCGAAGTAG CACTCAGTTG 
AGTCAAGAAC TGGCTGCAGA ATTTTTGGGA TCAGTTAGGG CAAATGCTAC AGCTACATGC 
TACCAGGTTA CATCTCTTAA TGAAAACGCT GCAAAGAAAC TGATGAAAAG ACATCCAGCA 
AAATGCGTAT CTTATACTCG GGACCATCTG ATTCGTACGT ATCCAAGTGC CAAGCACTAT 
GACTCGTCCA ACTTTAACCC CATCAACTGC TGGGCTCATG GAATGCAAAT GGTTGCTTTA 
AATTTTCAAA CTCCTGATGT TATAATGGCA GTTAACCAAG CGATGTTCGA GCAATCTGGA 
AACTGTGGAT ATCAACTGAA ACCACGTTGT CTTTGGGATG AATCTCATCT TTTGTACAAC 
AAATTTTTGC CTCTCTCCAA AGACATTGCC GGCCATTCTG CTTTGTTACT AAACTTAACT 
ATTATATCCG GACAGCACGT GTATCCAAAC ACCCATTACG CCAGTTTATA TGTCGAAATT 
GAAGTCATCG GTATTCACAA CGACTGTGTT CGTGAGAAAT CAAAAGTGGT CCAGAGAAAT 
TCTGTGAATC CAATCTGGAA CCACACCACT CAACTTCGAA TAGCTTGTGT TGACTTAGCA 
TTCCTTCGCA TAGCAGTATG TGATAGTGGG CAAAATGGAA GAGTCGTTGC TCACCGAGTT 
GTTCCTGTCA AATGCATTCG TCCGGGTTTC CGACATCTCC CACTCAGAAC TCCAACTAAT 
CTTCCGATCG ACAACGCTAT GATATTTTTA AGAACAAGAT TCGAGCAAGA AGAACACATC 
TACCTCCACG ACGATGATTC AAACACCTAT TGTAATCTAG AACATACGCT GGCATACCGC 
ACAGACTTAA CACCCAATTT ATCTCCAACG CCAATTTTGA AGAAACAGAT CTTTGTTCTT 
CGAATTACAG GCGCGTTTGC TGATGAAACA GCAATTACAG TGCACAGCGA GTCAGGAAGT 
ACAGTGAAGA CGGTGATGCA ACAGGCGCTG CTTAACGCTG GAAAGAATGC GGATCAGGTT 
GAAGAGTATG TACTCATCGA AGAATCACTG CCGGCACCTT CTGGGGAAGA TCCAATTGAG 
CAGAGAGTAC TTCCTCTGAA CGAGCCTATC ATGGACGCCG TGGCCTGCTG GAACGGTTCA 
ATGCGTCGTT TTGTGTTACG AAAAAAAGGC AGTGACCCAT CATCTAGAGC TTGGATCACT 
TCAATTATAA AATCCGGAAC AAGTGGAAGC TCCACGTCAG TCTCACCAAG TCCGTTGACC 
AAAGATGGAC ACGTAAAGAG TGCATCAAGC AATCAACTTC ATGGACGATC ATTGGACACT 
GACGCTTTTG GAGAGCACCT TGAAGTGACA GAAGGGTTGA ACCCTCGTGC AAGATCAATG 
GGCGACACAT TTCTGGTATG TGTTCACAAT GTTAGTGAAG ATCAGCCTTA TGCAATCCTA 
CGTGCTGGAA TCCACAGCAC TGCCGCCGAC ATTATTCGTC AGGTGTTTGT TAAAGCTCGC 
CGTTCAAATG TAGACGATTC CGAGTTTGTT CTTGTTGAAG AAACCTGCGA TGATCCAAAA 
CTCAATCAAG GCCAAATGAC TCCAAAGTAT CCAAACAACC GCACCACAAG CCGAGTGTTA 
GGACAAAACG AAAATGTTTG GAAAGCACAA TCGAGGTGGA AGTCAATGGG CAGATTTGTA 
CTTGAAAACC GAAAGGACAC AGTGCACGCA ACCCTCGAAA AAGTGAGATC ATTTATATCA 
AAACTGGAAG CTGCAAAAGC AAGCATGGAT CCCAGACACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123141.2
CDS	1..10485
Translation	MSLRQASHKFPSVDSMPTSRDSSMETTDRYPFGYTTELARTEPSASTAKRLRQRLQRQQSGQSGLSGRRYTGESSECSSLVGTPTDTGRSFTFGAAQRVMRERDDRKPSVTQNLSEIMLEAIHYEDVNLVERLLTAHSSKQPMSTSPSIGSTNTLTDLAQRRHGAGAHRRSNASSTVSTHSGGRSTCAMMNTLHMAVAHKQRDIVELLLKNGYDPNTPASCHCKGNCTATGNIPLTSIIPRTHSMTPELCSTCSQLRVVSIVDQTPLGVAVRSQSSELIALLIAYGGDVNLGDEDGNTPLMLAVRESPLSWPCLHTLIFFGAQIEQKNMRGICPLDLAPELKKLQQTCVEELFKNACSGDCEPPSRSTPGPSAKNWNRLQVDNDPVIDCVSWEQAWELLKKMACNPECLDNIVNSLSKFATQMENTPSSVDRDQFDAHMGGLLHKMIHTAIEEYESSTPTYKKQKKLHLTWLLSQIATFCFTFLQKSGTSRQFSALNTLNKIIDAGLVHDLFAFNDVVFHSSRILNRCHAFDSDEPPTPSGESFNSNADQNSTVDHVFIYGAGYKDQSLLPSPTVAPTRHKSDLMNSFSEMDPSQVIVALHNAITMQNREVGSRSVCSPAHRWRQCCAHCTQILVARLLLFLTHAKKFRSRLSERHQLRSLVALLEPTLEPQLLCLLLQVLALIALDPSTHTTLIDIQIDDVLIQMLLPADDWYYTNHSTKYGTFVKYHAARVLVYVGMGDRVGSRVNLFNMMTFEPSAKSGSQQNEDDYICETCATPRSRSTFSRSAVSVEGVLLKVLAEVAELVRQSQHLSASEPITEESPSAGSPPAVLPNQAEQKSINEKNAEKLTQLGCQILISLEQLEAHLCKLGLVLDSVLLLRLLLHKLSWDLGLVTKKRVAVVDHVYKPITDPRAHSSCSLGNNRREDSKRDKNYLRVDQGSRASKRVHIRRSSSVEIIRPKRLSSGAKDIKNRERRKRLGTDTSSGSNRSKKTNSSSSSVQKHLPKYIQSLFRSRMGTDPCKRTSRSESTLPHRDSNESNTSGSDAVLEFTRKLQNYPLTRREAQRLTYRSNGQQDSNDSKMGPRTLGYVYLPELEIQGASPPRSPGPLTGNSLEEGTTMLLDTRRPSSPQAIPGLPLIEIRRPSALSQFEFGYFVNSPELSGSEVSDCAPLLLSGQIGGSRKSSDETSLGGWSSRASSVMSQRSSRSSVGLRLSTFSGGTSIASDNSGPFLFSFVLRKRASTIGTRIPIPRRALSRSSGDSLRVPDRESPLHLASMTEMNPDFQCVRQLILNLLNVYTKRNDNVVSTMKECADVLRQILNSPQHPTVKNWCAEIIHVVSTHVEEEEPTTAENNEQINDEYLEFQDQVISGSLPCPKEEAAYLASIQLSVEEQWPSNKRTQTIRRHLLKGQFGRIRDLAQKIMVTPWEVDQNLYCTPPRFPNESANASRAQSVVEEIQHRSRTPTLLRCITNTDGLMSEEMQAQCLPVDLRGDRRTIKLVKERKRKLFHSQVYESEIGMKKLYIQTAKKLAAFGCKVFQVKELLHGRTLRKTLRLLCLSSAQLCLLDGSTKLVLKRQHASTLQQWRVGGGVSKHQLLLEFRGTKWQLIAPSYNALKSISMTLWEIMQNSASNSIQRSLNQSMHRSHTEATVSTSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKVRSFISKLEAAKASMDPRHE

Target ORF information:

RefSeq Version	NM_001129669.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001129669.3

ORF Insert Sequence:

ATGTCCTTAC GCCAAGCATC ACACAAATTT CCATCGGTGG ACTCAATGCC AACATCACGG 
GATTCATCGA TGGAAACAAC CGACCGGTAC CCGTTTGGGT ATACGACCGA ATTGGCACGT 
ACCGAACCAT CTGCATCAAC TGCCAAACGT CTTCGACAAA GGTTACAGCG ACAGCAAAGT 
GGTCAATCAG GATTATCCGG TCGACGGTAT ACTGGAGAAA GCAGCGAGTG CTCATCACTT 
GTCGGAACAC CCACAGACAC TGGCAGATCA TTTACATTTG GAGCAGCACA AAGAGTCATG 
CGGGAACGGG ATGACCGCAA ACCATCAGTT ACCCAGAACT TGTCAGAAAT AATGCTCGAG 
GCAATACATT ATGAAGATGT GAACTTGGTG GAAAGGTTGC TCACTGCGCA TAGTTCAAAA 
CAACCAATGT CAACATCTCC GTCAATTGGT AGCACAAACA CATTGACGGA TCTTGCTCAA 
CGTAGACACG GTGCGGGGGC ACACCGACGT TCCAATGCCA GTAGTACGGT GTCCACACAT 
TCCGGAGGAA GATCCACGTG TGCTATGATG AACACGTTGC ACATGGCTGT GGCTCATAAA 
CAACGAGATA TAGTGGAACT TTTGCTTAAA AACGGCTACG ATCCCAATAC TCCCGCATCG 
TGTCACTGTA AGGGAAATTG CACGGCGACT GGGAATATTC CACTGACTTC GATCATTCCG 
AGAACTCATT CAATGACGCC AGAATTGTGT TCGACGTGCT CTCAACTGAG GGTTGTGTCA 
ATCGTGGATC AAACACCGCT TGGTGTGGCA GTCAGATCAC AGTCCAGCGA ATTGATAGCA 
TTGTTGATTG CCTACGGAGG AGATGTTAAT TTAGGTGATG AAGATGGCAA CACGCCGTTG 
ATGCTAGCCG TACGAGAATC ACCTCTGTCT TGGCCTTGCC TGCACACGCT CATTTTCTTT 
GGAGCTCAAA TTGAACAAAA GAACATGCGC GGAATATGCC CTCTTGATCT TGCTCCAGAG 
CTGAAGAAAC TTCAACAAAC TTGCGTTGAA GAGCTTTTCA AAAATGCGTG CTCGGGTGAT 
TGTGAACCAC CATCTCGATC TACACCGGGA CCATCAGCCA AAAACTGGAA TAGACTACAA 
GTTGACAATG ATCCTGTAAT TGACTGCGTC AGTTGGGAAC AAGCATGGGA ATTGCTGAAG 
AAGATGGCAT GTAATCCAGA ATGTCTAGAT AACATCGTGA ATTCATTGTC AAAATTTGCA 
ACCCAAATGG AGAACACCCC GTCAAGCGTT GATCGTGATC AATTCGATGC ACACATGGGT 
GGCCTACTAC ATAAAATGAT TCATACAGCG ATTGAGGAGT ACGAGTCTTC GACACCAACC 
TACAAAAAGC AGAAGAAACT CCATCTCACC TGGCTGCTCA GCCAAATTGC CACGTTTTGT 
TTTACATTTC TGCAGAAAAG TGGAACGAGC CGACAATTCT CAGCACTGAA CACGTTGAAT 
AAAATTATTG ATGCTGGTTT AGTACATGAT TTGTTCGCAT TCAACGACGT TGTCTTTCAT 
TCTTCAAGAA TTTTGAACCG ATGCCATGCA TTTGATTCCG ACGAACCACC AACTCCATCT 
GGCGAATCTT TCAACTCAAA TGCAGACCAA AACTCAACTG TCGATCATGT TTTCATTTAC 
GGAGCTGGCT ATAAAGACCA ATCGTTATTA CCTTCTCCTA CGGTGGCACC AACTCGACAC 
AAGTCCGACT TAATGAATTC TTTCAGCGAA ATGGATCCAA GCCAGGTCAT TGTGGCCTTG 
CACAACGCGA TAACAATGCA AAACCGGGAA GTAGGGTCAA GATCTGTTTG TAGTCCGGCT 
CATCGATGGA GGCAGTGTTG TGCTCATTGT ACACAGATAT TGGTGGCCAG ATTATTGCTA 
TTCTTAACAC ACGCAAAAAA ATTTCGAAGT CGGTTGTCTG AACGACATCA GCTCAGAAGC 
TTGGTAGCTT TGTTAGAACC AACTTTGGAA CCTCAACTCT TATGTCTTTT ACTTCAAGTT 
CTAGCTCTTA TAGCGTTAGA TCCATCTACC CACACAACTT TGATAGACAT TCAAATTGAC 
GACGTCTTGA TTCAAATGCT ACTCCCAGCA GATGATTGGT ACTACACTAA TCACAGCACT 
AAATATGGAA CTTTTGTAAA GTACCATGCT GCTAGAGTTT TAGTGTACGT TGGAATGGGT 
GATCGAGTAG GAAGTCGAGT CAATCTGTTC AACATGATGA CGTTTGAGCC ATCTGCAAAA 
AGCGGGAGTC AACAAAACGA GGACGACTAC ATTTGTGAGA CATGTGCCAC ACCGCGCAGC 
CGAAGTACAT TTTCAAGATC AGCAGTTTCA GTGGAAGGTG TTCTGTTAAA AGTGTTGGCA 
GAAGTTGCTG AGCTTGTACG TCAGAGTCAA CACCTATCAG CAAGTGAACC AATAACTGAA 
GAATCGCCCA GTGCAGGATC TCCACCCGCT GTCCTACCAA ATCAGGCTGA ACAGAAAAGT 
ATTAACGAAA AAAATGCAGA GAAACTTACA CAACTTGGGT GCCAAATTTT GATCTCATTG 
GAACAACTTG AAGCTCATTT ATGCAAACTT GGACTGGTAC TTGATTCGGT TCTACTTCTC 
CGTCTACTTC TTCATAAGTT GTCATGGGAT CTTGGATTAG TCACAAAAAA GAGGGTTGCT 
GTGGTGGATC ATGTTTACAA ACCAATCACG GATCCGAGAG CTCATTCTAG CTGCAGTCTT 
GGAAACAACA GGCGAGAAGA TAGCAAACGG GACAAAAATT ACTTGCGTGT TGATCAGGGA 
TCTCGTGCGT CAAAGAGAGT GCATATCAGA CGATCTAGTT CAGTAGAGAT TATCAGGCCA 
AAACGACTCA GTTCCGGAGC AAAAGATATC AAAAATCGAG AACGGAGGAA AAGGCTGGGA 
ACAGATACAA GTAGCGGGTC GAATCGTTCC AAGAAAACCA ACTCCTCGTC TTCATCTGTT 
CAGAAACACT TGCCAAAATA CATTCAATCA TTATTCCGAA GTCGGATGGG AACTGATCCA 
TGCAAGAGGA CATCGAGAAG TGAATCCACT TTACCGCACA GAGACTCTAA TGAGAGTAAT 
ACAAGTGGAA GTGATGCTGT TCTTGAGTTC ACAAGAAAAC TTCAAAATTA TCCGTTGACG 
AGACGCGAAG CGCAAAGATT GACGTATCGA AGCAATGGGC AACAAGATTC AAATGACTCG 
AAGATGGGAC CACGAACTTT AGGATACGTT TACCTCCCAG AACTAGAAAT TCAAGGTGCA 
AGTCCGCCAA GATCACCGGG ACCTCTAACT GGAAACTCGT TAGAAGAAGG AACCACAATG 
CTTTTGGACA CAAGACGGCC GAGCAGCCCA CAAGCAATCC CCGGCCTTCC TTTAATTGAA 
ATTCGTCGGC CCTCAGCTCT CTCTCAATTT GAATTTGGAT ACTTTGTAAA CTCACCAGAA 
TTATCAGGAA GTGAAGTATC TGATTGTGCC CCATTACTTC TCAGTGGTCA AATTGGTGGA 
AGCCGAAAAA GCTCCGACGA AACATCATTG GGAGGATGGT CCAGCCGTGC TTCATCGGTA 
ATGAGTCAAA GGTCGTCCAG ATCCAGTGTT GGCTTGAGGT TATCAACATT TTCTGGAGGG 
ACGAGTATTG CTTCGGATAA CTCTGGGCCA TTTTTGTTCA GTTTTGTTTT GAGAAAACGT 
GCATCTACTA TAGGAACTAG AATACCAATA CCTAGGAGAG CTTTGTCAAG ATCTTCAGGA 
GACAGTCTGA GAGTACCTGA TAGAGAAAGT CCACTTCATT TAGCAAGTAT GACAGAGATG 
AATCCGGATT TTCAATGTGT TCGACAGCTC ATCTTGAACC TTTTGAATGT TTACACGAAA 
AGAAATGACA ATGTGGTTTC TACAATGAAG GAATGTGCGG ATGTTCTTCG TCAAATCCTA 
AACTCTCCTC AACATCCAAC TGTCAAGAAT TGGTGTGCCG AAATAATTCA TGTAGTCAGT 
ACTCATGTAG AAGAAGAGGA ACCAACGACG GCAGAAAATA ATGAACAAAT TAATGACGAG 
TATCTGGAAT TTCAAGACCA AGTGATATCA GGAAGTTTAC CATGCCCGAA GGAAGAGGCT 
GCATACCTTG CAAGTATTCA GTTATCAGTT GAAGAGCAGT GGCCGAGTAA TAAAAGGACC 
CAGACAATTA GAAGACATTT ATTGAAAGGA CAGTTTGGTC GAATCCGGGA TCTCGCCCAA 
AAGATCATGG TGACTCCATG GGAAGTTGAT CAAAACTTGT ACTGCACACC ACCCAGATTT 
CCCAATGAAT CGGCAAATGC TAGTAGAGCA CAATCAGTGG TTGAGGAAAT TCAGCACAGA 
TCAAGAACTC CAACTCTTCT GCGATGTATC ACAAACACAG ACGGCTTAAT GTCAGAAGAG 
ATGCAAGCTC AGTGCCTTCC CGTCGATCTA CGTGGTGATC GTAGAACTAT CAAGCTGGTC 
AAGGAGAGGA AGCGAAAATT GTTCCATAGT CAAGTCTATG AAAGTGAAAT TGGCATGAAG 
AAATTGTACA TTCAGACCGC CAAAAAACTA GCCGCATTTG GTTGTAAGGT TTTTCAAGTT 
AAAGAGTTGC TTCACGGACG AACACTCAGA AAAACTCTTC GACTGCTTTG CCTTTCTTCT 
GCTCAATTGT GTCTTCTCGA CGGATCAACA AAACTTGTGC TTAAACGGCA GCACGCATCG 
ACGTTACAAC AGTGGAGAGT AGGTGGTGGA GTTAGTAAGC ATCAACTATT GCTCGAATTC 
CGTGGAACCA AGTGGCAACT TATTGCACCG TCGTATAATG CACTGAAATC GATATCGATG 
ACATTATGGG AGATTATGCA AAATTCTGCT AGCAATTCAA TCCAGCGGAG TCTCAACCAG 
TCAATGCATC GTTCGCACAC CGAGGCGACT GTCTCAACAT CACGTAGTGA CTCAACCTCT 
TCAACGCAAT CAGCCACAGC TGGATTCATT CTCAATGAAG AACCAATCAC ACTGTTCCGA 
CTTGAACTCG AACGACTTCA ATATATTCTT CATTTCCCTG AAGAAGTTGC ATTCCAACTA 
TCTTCTACTG AGTATCAGCT TTTCTACTCA ATACAACCCA TGGATTATGT TCGTTACGTC 
AGTTGCGATC TCACATCTGT TCCAGTGTCA GAAAATCCAA GTCCAGTGCG AAATCTTGTG 
AAAAGGCTAT CAGAGGTATC ATCCTGGATA ACACATGTTA TTGTCAGTCA GCCAACACAT 
GACGATCGAA AGGTTGCTCT TACTGCAATT TTGAGAATTG TGGAAACTTG CTGGAATATT 
GGAAACTTTA ATGCAGCTGT TGAGGTTCTT ATGGGTTTAA AGAGTGAAAA ACTCAGACCA 
TTCTGGCTTT CTTTGCGACA AGAGGAAAAG TCTCAGTTTG ATTCGCTTTG CGAAACCTTG 
CTTCCTGCAA ACCAAGCTCT CCCATCTCAA GCTTACATCA ACGCGGTCCA ACGAGCACTG 
AGAATGCCAC AATCTCGTGT GATTCCATTT TTTGGAATAT TTCTTCGTGA TCTCTACGCA 
ATTGTGAATG ACTTGCCGAA TATCGTTGTG ATTGGACAAG AAGGTGAAAC CCAAAAGCTG 
GAATTCATGA GCGATCCCAA TGGCGAAGAT CATTTTTCGT CGAGAATTGG AGTGGGTGGG 
CTGTTGAATG CTGATAAAAT CAACTTGGTG GCAATTGTTT TGGACAACTT GGAATTATTC 
CATCGTCACA GCAGGACAAT GATCAAATTG TTAGAAGAGC AAGCAGTCCC ACCAATTCAA 
ATTCCTCAGA ACGAGCGGGA GCAAAAAGAA AAGGAAGCAA AAACATATGA ACCAGTACAA 
GTGGTTCGCG GCTCTTCTCA TGGAGTGGCT CTCATCCCTC TTGATACCCT AACATTCGAT 
CTTGATGTTA TCCAACGACT CCAGCACGGA ACAACAGTGA TCCATTACGA ACCTGATTCG 
GGAAGAAGCA ATTTGTGTCT GCTGCGGTTA GACCCTAGTT GCGGTCAAAT AAACTGGCAT 
AAGATTTCAT ATTCAGTTAA CAAAGATCCC AAAGAAAAGG ATGTGCTCGC CAAAGTATCC 
GTTTCAAATC TGCAGCCGCT AGACTCTGGC CGAGGGGCTC CGTCACCAAT GCCATCTGGA 
AGAACACCAG GAACTGGAGG AGTTGGAGTA GAGGAGGGAG AACTCAAATT AAGTGTTGTG 
AAAGGAGTCG AACTAGTTGA TAGTTATGAT ATTGATATTG AGGCAATTTA CCGTCGTCAT 
TCTATGGAAG AGATGTCAGT TCCTGTTAGC TGTTGGAAAG TTAGTCATGG TCAGTTGTTA 
TCGGATAATG AGTTCATCTA CTTTTTGGCC CCTCAACAAA TTGCTCAATT TTGGACCAAC 
GGGTTGCAAT CTGTAGTTAA AAGTCTCCAA GGACAGCAAA GGTACCCTGA TCGGCGAATG 
TTATGGATCA AAAATGTCTA CTTATCACTT TACGAGATAA CGGGCGAATC AAACTGTGGA 
CCACGGCCAT TTGAAGCACT TCAGGCATTT GGGCTGAGTC AAACAAATAC TAACGCAACA 
CGACCCAATG ACTCGTCGCT GTCCAGTGAG CCAGGAGGAG CTAAAAGCAG ACTGAAGAAC 
CTTAAAAATG CTATGCAAAA GAAGCTTCGT GGTGCATCGC GCGAAGGAAG CAGATCTCAG 
TCACCTCAAC CTCATTCTCC TCTTGTCCGA CCACCATCAA TAAAATCGCA AATATCGTCA 
CAATCTGGTC CGCCGGGTCC GAACTCTCCA GGCTATTTGC TGAAACCACG TGGAGAACCT 
GCCAATAGTG ATGCAGGAGA CATTGATAGC ATTTATACAC CTCGATCAAG AACTCCAACA 
AGCAGCTCCT ATGGAGGGCG ATCCGTTGGA GGGAGATCAT GTAAATCGTG GAGATCAAGA 
GGAGGAGAGA CACCAAATTC GGGTTCCATA AGCAGCTCGG GTCAGATGTC AATTCAAGTG 
TCTGGTCTGA GTGGACCATC GGGAAAAGAG TTTCAGGAAA AACCATTAAC ACTTGTAGAG 
TTCGCTGAAC TTTTTCGATT GTTCAATACA AGAATGAGAA AAGATTTGAG AGATGTGTTC 
AATGATGTCC TGTCTACGGC AACTACTCCT CAGCATTGTC CTAAAAGAGA ACGTGATCGC 
CACAGTCCGA GAATGCAGTC AAGGCTGGCG AGTGTCTCCA ACTCGTACAA TGCAGATTTT 
CTGTCTAACG ATTTCTTGAC TCGGAATACG GCAGTAACGT CCCATCATAT CAGTGAAAAG 
CAAAACAAAA TCTACAATGC ATTGGCACTT GCGAGTGTTA ATAGCATGGG CGGACTCATG 
GACACTTCCC GGTCGTCGAT GCTGACGCCG CAAATGCTGC GCGCTTTTGT CAACACTCAT 
CAGATGGAGC AGATTGACGA GCAAACGGCG ATTAAGCTTA TTCAGGATCA TGAACCTGAT 
GGTATCTGTC GACAAAAGAA TCAAATGTCA TTCGAAGGAT TCACTAGATT CCTTTGCGAT 
CCTGTCAACT TTGCATTCGT ACCGGAAACA ATTGAACCAG ATGAAGAGGA CCTACGGTAC 
CCGTTGTCTC ATTATTACAT CAATTCAAGT CACAACACCT ATTTGACGGG ACATCAACTC 
AAGGGACCCA GTAGCTCGGA GATGTACAGA CAGGTTCTCC TCACCGGGTG TCGGTGTGTC 
GAGCTTGATT GTTGGGATGG AGATGACGGA TTGCCATTGA TTTATCATGG ACACACATTG 
GTTAGCAAGA TTGGGTTTAG ACAGGTGGTC GAAATTATCA AAAAATCTGC ATTCATAACA 
TCGGATCTTC CTGTGATATT ATCAATTGAG AATCATTGTT CCCTACAACA ACAGGCGAAA 
ATGGCACAGA TGTTCAAAAC AGTTCTTGGA GATTTACTAG TATCAAACTT CCTTTTTGAA 
GCTGATTTCT CGGACTCTCC ACGTCTTCCA TGCCCTCTCC AAATGAAAAA TAAGATCTTG 
ATAAAAAATA AGAAAATGAT AGTCGACCCA CCAACTCCAT TGCCAATGAT TGAACGAGGA 
GCTGTGCAGC GTGGTGAAAC TCAATTAAAT CTTCACAGAA AACAGAGTAA AAACTCCTAC 
GAAAGCAGTA CGGTAGATGA AGTGGAAGAT GACGACCTAG ATGAGTTTCT TGACGATGAA 
GAAAATGAGG AGGATGACCA AGAAGAGGTT CAAGTCAGAA GTGAAAAAGA AGACAGTCCG 
AAGACATCAA AACGTGCAGA GAAATCGGCT AGAAACATCA AACAGCAAGA TTCGTTATGT 
AGCGATCATT CGGTAGAGCA GGCAAAACCT TCCACAAGCA AGACCACATC CAAAACCAAC 
GATAGAAAGA CTGAAGATGA AGTACTTTAC GCTCAACTTG CTCAAAATGC CATCCGAAAC 
CAACAACCAA GAAAGAACAA TACTGGAGTC CAAATAGCGC CAGAGTTGTC AGATATTGTC 
ATTTACATGC AAGCGACAAA ATTCAAAGGA TTTCCCCCAG TCGATGGCAT TCAATCACCA 
AGGATAATGG AGGAAGGGCC AGCTTCTGCA AGTCTTTCAT TTTCATCACG GGCTCGTACG 
CCATCAAATC TGCTGAATAC ACCAGCCCCT CCACGACGAC AGCGAAGTAG CACTCAGTTG 
AGTCAAGAAC TGGCTGCAGA ATTTTTGGGA TCAGTTAGGG CAAATGCTAC AGCTACATGC 
TACCAGGTTA CATCTCTTAA TGAAAACGCT GCAAAGAAAC TGATGAAAAG ACATCCAGCA 
AAATGCGTAT CTTATACTCG GGACCATCTG ATTCGTACGT ATCCAAGTGC CAAGCACTAT 
GACTCGTCCA ACTTTAACCC CATCAACTGC TGGGCTCATG GAATGCAAAT GGTTGCTTTA 
AATTTTCAAA CTCCTGATGT TATAATGGCA GTTAACCAAG CGATGTTCGA GCAATCTGGA 
AACTGTGGAT ATCAACTGAA ACCACGTTGT CTTTGGGATG AATCTCATCT TTTGTACAAC 
AAATTTTTGC CTCTCTCCAA AGACATTGCC GGCCATTCTG CTTTGTTACT AAACTTAACT 
ATTATATCCG GACAGCACGT GTATCCAAAC ACCCATTACG CCAGTTTATA TGTCGAAATT 
GAAGTCATCG GTATTCACAA CGACTGTGTT CGTGAGAAAT CAAAAGTGGT CCAGAGAAAT 
TCTGTGAATC CAATCTGGAA CCACACCACT CAACTTCGAA TAGCTTGTGT TGACTTAGCA 
TTCCTTCGCA TAGCAGTATG TGATAGTGGG CAAAATGGAA GAGTCGTTGC TCACCGAGTT 
GTTCCTGTCA AATGCATTCG TCCGGGTTTC CGACATCTCC CACTCAGAAC TCCAACTAAT 
CTTCCGATCG ACAACGCTAT GATATTTTTA AGAACAAGAT TCGAGCAAGA AGAACACATC 
TACCTCCACG ACGATGATTC AAACACCTAT TGTAATCTAG AACATACGCT GGCATACCGC 
ACAGACTTAA CACCCAATTT ATCTCCAACG CCAATTTTGA AGAAACAGAT CTTTGTTCTT 
CGAATTACAG GCGCGTTTGC TGATGAAACA GCAATTACAG TGCACAGCGA GTCAGGAAGT 
ACAGTGAAGA CGGTGATGCA ACAGGCGCTG CTTAACGCTG GAAAGAATGC GGATCAGGTT 
GAAGAGTATG TACTCATCGA AGAATCACTG CCGGCACCTT CTGGGGAAGA TCCAATTGAG 
CAGAGAGTAC TTCCTCTGAA CGAGCCTATC ATGGACGCCG TGGCCTGCTG GAACGGTTCA 
ATGCGTCGTT TTGTGTTACG AAAAAAAGGC AGTGACCCAT CATCTAGAGC TTGGATCACT 
TCAATTATAA AATCCGGAAC AAGTGGAAGC TCCACGTCAG TCTCACCAAG TCCGTTGACC 
AAAGATGGAC ACGTAAAGAG TGCATCAAGC AATCAACTTC ATGGACGATC ATTGGACACT 
GACGCTTTTG GAGAGCACCT TGAAGTGACA GAAGGGTTGA ACCCTCGTGC AAGATCAATG 
GGCGACACAT TTCTGGTATG TGTTCACAAT GTTAGTGAAG ATCAGCCTTA TGCAATCCTA 
CGTGCTGGAA TCCACAGCAC TGCCGCCGAC ATTATTCGTC AGGTGTTTGT TAAAGCTCGC 
CGTTCAAATG TAGACGATTC CGAGTTTGTT CTTGTTGAAG AAACCTGCGA TGATCCAAAA 
CTCAATCAAG GCCAAATGAC TCCAAAGTAT CCAAACAACC GCACCACAAG CCGAGTGTTA 
GGACAAAACG AAAATGTTTG GAAAGCACAA TCGAGGTGGA AGTCAATGGG CAGATTTGTA 
CTTGAAAACC GAAAGGACAC AGTGCACGCA ACCCTCGAAA AAGTGAGATC ATTTATATCA 
AAACTGGAAG CTGCAAAAGC AAGCATGGAT CCCAGACACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_001373492.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360635.1 Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.

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CloneID	OCa851222
Clone ID Related Accession (Same CDS sequence)	NM_001373492.1
Accession Version	NM_001373492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	PhosphoLipase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on the 5' end.

ATGTCCTTAC GCCAAGCATC ACACAAATTT CCATCGGTGG ACTCAATGCC AACATCACGG 
GATTCATCGA TGGAAACAAC CGACCGGTAC CCGTTTGGGT ATACGACCGA ATTGGCACGT 
ACCGAACCAT CTGCATCAAC TGCCAAACGT CTTCGACAAA GGTTACAGCG ACAGCAAAGT 
GGTCAATCAG GATTATCCGG TCGACGGTAT ACTGGAGAAA GCAGCGAGTG CTCATCACTT 
GTCGGAACAC CCACAGACAC TGGCAGATCA TTTACATTTG GAGCAGCACA AAGAGTCATG 
CGGGAACGGG ATGACCGCAA ACCATCAGTT ACCCAGAACT TGTCAGAAAT AATGCTCGAG 
GCAATACATT ATGAAGATGT GAACTTGGTG GAAAGGTTGC TCACTGCGCA TAGTTCAAAA 
CAACCAATGT CAACATCTCC GTCAATTGGT AGCACAAACA CATTGACGGA TCTTGCTCAA 
CGTAGACACG GTGCGGGGGC ACACCGACGT TCCAATGCCA GTAGTACGGT GTCCACACAT 
TCCGGAGGAA GATCCACGTG TGCTATGATG AACACGTTGC ACATGGCTGT GGCTCATAAA 
CAACGAGATA TAGTGGAACT TTTGCTTAAA AACGGCTACG ATCCCAATAC TCCCGCATCG 
TGTCACTGTA AGGGAAATTG CACGGCGACT GGGAATATTC CACTGACTTC GATCATTCCG 
AGAACTCATT CAATGACGCC AGAATTGTGT TCGACGTGCT CTCAACTGAG GGTTGTGTCA 
ATCGTGGATC AAACACCGCT TGGTGTGGCA GTCAGATCAC AGTCCAGCGA ATTGATAGCA 
TTGTTGATTG CCTACGGAGG AGATGTTAAT TTAGGTGATG AAGATGGCAA CACGCCGTTG 
ATGCTAGCCG TACGAGAATC ACCTCTGTCT TGGCCTTGCC TGCACACGCT CATTTTCTTT 
GGAGCTCAAA TTGAACAAAA GAACATGCGC GGAATATGCC CTCTTGATCT TGCTCCAGAG 
CTGAAGAAAC TTCAACAAAC TTGCGTTGAA GAGCTTTTCA AAAATGCGTG CTCGGGTGAT 
TGTGAACCAC CATCTCGATC TACACCGGGA CCATCAGCCA AAAACTGGAA TAGACTACAA 
GTTGACAATG ATCCTGTAAT TGACTGCGTC AGTTGGGAAC AAGCATGGGA ATTGCTGAAG 
AAGATGGCAT GTAATCCAGA ATGTCTAGAT AACATCGTGA ATTCATTGTC AAAATTTGCA 
ACCCAAATGG AGAACACCCC GTCAAGCGTT GATCGTGATC AATTCGATGC ACACATGGGT 
GGCCTACTAC ATAAAATGAT TCATACAGCG ATTGAGGAGT ACGAGTCTTC GACACCAACC 
TACAAAAAGC AGAAGAAACT CCATCTCACC TGGCTGCTCA GCCAAATTGC CACGTTTTGT 
TTTACATTTC TGCAGAAAAG TGGAACGAGC CGACAATTCT CAGCACTGAA CACGTTGAAT 
AAAATTATTG ATGCTGGTTT AGTACATGAT TTGTTCGCAT TCAACGACGT TGTCTTTCAT 
TCTTCAAGAA TTTTGAACCG ATGCCATGCA TTTGATTCCG ACGAACCACC AACTCCATCT 
GGCGAATCTT TCAACTCAAA TGCAGACCAA AACTCAACTG TCGATCATGT TTTCATTTAC 
GGAGCTGGCT ATAAAGACCA ATCGTTATTA CCTTCTCCTA CGGTGGCACC AACTCGACAC 
AAGTCCGACT TAATGAATTC TTTCAGCGAA ATGGATCCAA GCCAGGTCAT TGTGGCCTTG 
CACAACGCGA TAACAATGCA AAACCGGGAA GTAGGGTCAA GATCTGTTTG TAGTCCGGCT 
CATCGATGGA GGCAGTGTTG TGCTCATTGT ACACAGATAT TGGTGGCCAG ATTATTGCTA 
TTCTTAACAC ACGCAAAAAA ATTTCGAAGT CGGTTGTCTG AACGACATCA GCTCAGAAGC 
TTGGTAGCTT TGTTAGAACC AACTTTGGAA CCTCAACTCT TATGTCTTTT ACTTCAAGTT 
CTAGCTCTTA TAGCGTTAGA TCCATCTACC CACACAACTT TGATAGACAT TCAAATTGAC 
GACGTCTTGA TTCAAATGCT ACTCCCAGCA GATGATTGGT ACTACACTAA TCACAGCACT 
AAATATGGAA CTTTTGTAAA GTACCATGCT GCTAGAGTTT TAGTGTACGT TGGAATGGGT 
GATCGAGTAG GAAGTCGAGT CAATCTGTTC AACATGATGA CGTTTGAGCC ATCTGCAAAA 
AGCGGGAGTC AACAAAACGA GGACGACTAC ATTTGTGAGA CATGTGCCAC ACCGCGCAGC 
CGAAGTACAT TTTCAAGATC AGCAGTTTCA GTGGAAGGTG TTCTGTTAAA AGTGTTGGCA 
GAAGTTGCTG AGCTTGTACG TCAGAGTCAA CACCTATCAG CAAGTGAACC AATAACTGAA 
GAATCGCCCA GTGCAGGATC TCCACCCGCT GTCCTACCAA ATCAGGCTGA ACAGAAAAGT 
ATTAACGAAA AAAATGCAGA GAAACTTACA CAACTTGGGT GCCAAATTTT GATCTCATTG 
GAACAACTTG AAGCTCATTT ATGCAAACTT GGACTGGTAC TTGATTCGGT TCTACTTCTC 
CGTCTACTTC TTCATAAGTT GTCATGGGAT CTTGGATTAG TCACAAAAAA GAGGGTTGCT 
GTGGTGGATC ATGTTTACAA ACCAATCACG GATCCGAGAG CTCATTCTAG CTGCAGTCTT 
GGAAACAACA GAAACTTTGG TAAATCTAAA TCTTTTGATA GGCGAGAAGA TAGCAAACGG 
GACAAAAATT ACTTGCGTGT TGATCAGGGA TCTCGTGCGT CAAAGAGAGT GCATATCAGA 
CGATCTAGTT CAGTAGAGAT TATCAGGCCA AAACGACTCA GTTCCGGAGC AAAAGATATC 
AAAAATCGAG AACGGAGGAA AAGGCTGGGA ACAGATACAA GTAGCGGGTC GAATCGTTCC 
AAGAAAACCA ACTCCTCGTC TTCATCTGTT CAGAAACACT TGCCAAAATA CATTCAATCA 
TTATTCCGAA GTCGGATGGG AACTGATCCA TGCAAGAGGA CATCGAGAAG TGAATCCACT 
TTACCGCACA GAGACTCTAA TGAGAGTAAT ACAAGTGGAA GTGATGCTGT TCTTGAGTTC 
ACAAGAAAAC TTCAAAATTA TCCGTTGACG AGACGCGAAG CGCAAAGATT GACGTATCGA 
AGCAATGGGC AACAAGATTC AAATGACTCG AAGATGGGAC CACGAACTTT AGGATACGTT 
TACCTCCCAG AACTAGAAAT TCAAGGTGCA AGTCCGCCAA GATCACCGGG ACCTCTAACT 
GGAAACTCGT TAGAAGAAGG AACCACAATG CTTTTGGACA CAAGACGGCC GAGCAGCCCA 
CAAGCAATCC CCGGCCTTCC TTTAATTGAA ATTCGTCGGC CCTCAGCTCT CTCTCAATTT 
GAATTTGGAT ACTTTGTAAA CTCACCAGAA TTATCAGGAA GTGAAGTATC TGATTGTGCC 
CCATTACTTC TCAGTGGTCA AATTGGTGGA AGCCGAAAAA GCTCCGACGA AACATCATTG 
GGAGGATGGT CCAGCCGTGC TTCATCGGTA ATGAGTCAAA GGTCGTCCAG ATCCAGTGTT 
GGCTTGAGGT TATCAACATT TTCTGGAGGG ACGAGTATTG CTTCGGATAA CTCTGGGCCA 
TTTTTGTTCA GTTTTGTTTT GAGAAAACGT GCATCTACTA TAGGAACTAG AATACCAATA 
CCTAGGAGAG CTTTGTCAAG ATCTTCAGGA GACAGTCTGA GAGTACCTGA TAGAGAAAGT 
CCACTTCATT TAGCAAGTAT GACAGAGATG AATCCGGATT TTCAATGTGT TCGACAGCTC 
ATCTTGAACC TTTTGAATGT TTACACGAAA AGAAATGACA ATGTGGTTTC TACAATGAAG 
GAATGTGCGG ATGTTCTTCG TCAAATCCTA AACTCTCCTC AACATCCAAC TGTCAAGAAT 
TGGTGTGCCG AAATAATTCA TGTAGTCAGT ACTCATGTAG AAGAAGAGGA ACCAACGACG 
GCAGAAAATA ATGAACAAAT TAATGACGAG TATCTGGAAT TTCAAGACCA AGTGATATCA 
GGAAGTTTAC CATGCCCGAA GGAAGAGGCT GCATACCTTG CAAGTATTCA GTTATCAGTT 
GAAGAGCAGT GGCCGAGTAA TAAAAGGACC CAGACAATTA GAAGACATTT ATTGAAAGGA 
CAGTTTGGTC GAATCCGGGA TCTCGCCCAA AAGATCATGG TGACTCCATG GGAAGTTGAT 
CAAAACTTGT ACTGCACACC ACCCAGATTT CCCAATGAAT CGGCAAATGC TAGTAGAGCA 
CAATCAGTGG TTGAGGAAAT TCAGCACAGA TCAAGAACTC CAACTCTTCT GCGATGTATC 
ACAAACACAG ACGGCTTAAT GTCAGAAGAG ATGCAAGCTC AGTGCCTTCC CGTCGATCTA 
CGTGGTGATC GTAGAACTAT CAAGCTGGTC AAGGAGAGGA AGCGAAAATT GTTCCATAGT 
CAAGTCTATG AAAGTGAAAT TGGCATGAAG AAATTGTACA TTCAGACCGC CAAAAAACTA 
GCCGCATTTG GTTGTAAGGT TTTTCAAGTT AAAGAGTTGC TTCACGGACG AACACTCAGA 
AAAACTCTTC GACTGCTTTG CCTTTCTTCT GCTCAATTGT GTCTTCTCGA CGGATCAACA 
AAACTTGTGC TTAAACGGCA GCACGCATCG ACGTTACAAC AGTGGAGAGT AGGTGGTGGA 
GTTAGTAAGC ATCAACTATT GCTCGAATTC CGTGGAACCA AGTGGCAACT TATTGCACCG 
TCGTATAATG CACTGAAATC GATATCGATG ACATTATGGG AGATTATGCA AAATTCTGCT 
AGCAATTCAA TCCAGCGGAG TCTCAACCAG TCAATGCATC GTTCGCACAC CGAGGCGACT 
GTCTCAACAT CACGTAGTGA CTCAACCTCT TCAACGCAAT CAGCCACAGC TGGATTCATT 
CTCAATGAAG AACCAATCAC ACTGTTCCGA CTTGAACTCG AACGACTTCA ATATATTCTT 
CATTTCCCTG AAGAAGTTGC ATTCCAACTA TCTTCTACTG AGTATCAGCT TTTCTACTCA 
ATACAACCCA TGGATTATGT TCGTTACGTC AGTTGCGATC TCACATCTGT TCCAGTGTCA 
GAAAATCCAA GTCCAGTGCG AAATCTTGTG AAAAGGCTAT CAGAGGTATC ATCCTGGATA 
ACACATGTTA TTGTCAGTCA GCCAACACAT GACGATCGAA AGGTTGCTCT TACTGCAATT 
TTGAGAATTG TGGAAACTTG CTGGAATATT GGAAACTTTA ATGCAGCTGT TGAGGTTCTT 
ATGGGTTTAA AGAGTGAAAA ACTCAGACCA TTCTGGCTTT CTTTGCGACA AGAGGAAAAG 
TCTCAGTTTG ATTCGCTTTG CGAAACCTTG CTTCCTGCAA ACCAAGCTCT CCCATCTCAA 
GCTTACATCA ACGCGGTCCA ACGAGCACTG AGAATGCCAC AATCTCGTGT GATTCCATTT 
TTTGGAATAT TTCTTCGTGA TCTCTACGCA ATTGTGAATG ACTTGCCGAA TATCGTTGTG 
ATTGGACAAG AAGGTGAAAC CCAAAAGCTG GAATTCATGA GCGATCCCAA TGGCGAAGAT 
CATTTTTCGT CGAGAATTGG AGTGGGTGGG CTGTTGAATG CTGATAAAAT CAACTTGGTG 
GCAATTGTTT TGGACAACTT GGAATTATTC CATCGTCACA GCAGGACAAT GATCAAATTG 
TTAGAAGAGC AAGCAGTCCC ACCAATTCAA ATTCCTCAGA ACGAGCGGGA GCAAAAAGAA 
AAGGAAGCAA AAACATATGA ACCAGTACAA GTGGTTCGCG GCTCTTCTCA TGGAGTGGCT 
CTCATCCCTC TTGATACCCT AACATTCGAT CTTGATGTTA TCCAACGACT CCAGCACGGA 
ACAACAGTGA TCCATTACGA ACCTGATTCG GGAAGAAGCA ATTTGTGTCT GCTGCGGTTA 
GACCCTAGTT GCGGTCAAAT AAACTGGCAT AAGATTTCAT ATTCAGTTAA CAAAGATCCC 
AAAGAAAAGG ATGTGCTCGC CAAAGTATCC GTTTCAAATC TGCAGCCGCT AGACTCTGGC 
CGAGGGGCTC CGTCACCAAT GCCATCTGGA AGAACACCAG GAACTGGAGG AGTTGGAGTA 
GAGGAGGGAG AACTCAAATT AAGTGTTGTG AAAGGAGTCG AACTAGTTGA TAGTTATGAT 
ATTGATATTG AGGCAATTTA CCGTCGTCAT TCTATGGAAG AGATGTCAGT TCCTGTTAGC 
TGTTGGAAAG TTAGTCATGG TCAGTTGTTA TCGGATAATG AGTTCATCTA CTTTTTGGCC 
CCTCAACAAA TTGCTCAATT TTGGACCAAC GGGTTGCAAT CTGTAGTTAA AAGTCTCCAA 
GGACAGCAAA GGTACCCTGA TCGGCGAATG TTATGGATCA AAAATGTCTA CTTATCACTT 
TACGAGATAA CGGGCGAATC AAACTGTGGA CCACGGCCAT TTGAAGCACT TCAGGCATTT 
GGGCTGAGTC AAACAAATAC TAACGCAACA CGACCCAATG ACTCGTCGCT GTCCAGTGAG 
CCAGGAGGAG CTAAAAGCAG ACTGAAGAAC CTTAAAAATG CTATGCAAAA GAAGCTTCGT 
GGTGCATCGC GCGAAGGAAG CAGATCTCAG TCACCTCAAC CTCATTCTCC TCTTGTCCGA 
CCACCATCAA TAAAATCGCA AATATCGTCA CAATCTGGTC CGCCGGGTCC GAACTCTCCA 
GGCTATTTGC TGAAACCACG TGGAGAACCT GCCAATAGTG ATGCAGGAGA CATTGATAGC 
ATTTATACAC CTCGATCAAG AACTCCAACA AGCAGCTCCT ATGGAGGGCG ATCCGTTGGA 
GGGAGATCAT GTAAATCGTG GAGATCAAGA GGAGGAGAGA CACCAAATTC GGGTTCCATA 
AGCAGCTCGG GTCAGATGTC AATTCAAGTG TCTGGTCTGA GTGGACCATC GGGAAAAGAG 
TTTCAGGAAA AACCATTAAC ACTTGTAGAG TTCGCTGAAC TTTTTCGATT GTTCAATACA 
AGAATGAGAA AAGATTTGAG AGATGTGTTC AATGATGTCC TGTCTACGGC AACTACTCCT 
CAGCATTGTC CTAAAAGAGA ACGTGATCGC CACAGTCCGA GAATGCAGTC AAGGCTGGCG 
AGTGTCTCCA ACTCGTACAA TGCAGATTTT CTGTCTAACG ATTTCTTGAC TCGGAATACG 
GCAGTAACGT CCCATCATAT CAGTGAAAAG CAAAACAAAA TCTACAATGC ATTGGCACTT 
GCGAGTGTTA ATAGCATGGG CGGACTCATG GACACTTCCC GGTCGTCGAT GCTGACGCCG 
CAAATGCTGC GCGCTTTTGT CAACACTCAT CAGATGGAGC AGATTGACGA GCAAACGGCG 
ATTAAGCTTA TTCAGGATCA TGAACCTGAT GGTATCTGTC GACAAAAGAA TCAAATGTCA 
TTCGAAGGAT TCACTAGATT CCTTTGCGAT CCTGTCAACT TTGCATTCGT ACCGGAAACA 
ATTGAACCAG ATGAAGAGGA CCTACGGTAC CCGTTGTCTC ATTATTACAT CAATTCAAGT 
CACAACACCT ATTTGACGGG ACATCAACTC AAGGGACCCA GTAGCTCGGA GATGTACAGA 
CAGGTTCTCC TCACCGGGTG TCGGTGTGTC GAGCTTGATT GTTGGGATGG AGATGACGGA 
TTGCCATTGA TTTATCATGG ACACACATTG GTTAGCAAGA TTGGGTTTAG ACAGGTGGTC 
GAAATTATCA AAAAATCTGC ATTCATAACA TCGGATCTTC CTGTGATATT ATCAATTGAG 
AATCATTGTT CCCTACAACA ACAGGCGAAA ATGGCACAGA TGTTCAAAAC AGTTCTTGGA 
GATTTACTAG TATCAAACTT CCTTTTTGAA GCTGATTTCT CGGACTCTCC ACGTCTTCCA 
TGCCCTCTCC AAATGAAAAA TAAGATCTTG ATAAAAAATA AGAAAATGAT AGTCGACCCA 
CCAACTCCAT TGCCAATGAT TGAACGAGGA GCTGTGCAGC GTGGTGAAAC TCAATTAAAT 
CTTCACAGAA AACAGAGTAA AAACTCCTAC GAAAGCAGTA CGGTAGATGA AGTGGAAGAT 
GACGACCTAG ATGAGTTTCT TGACGATGAA GAAAATGAGG AGGATGACCA AGAAGAGGTT 
CAAGTCAGAA GTGAAAAAGA AGACAGTCCG AAGACATCAA AACGTGCAGA GAAATCGGCT 
AGAAACATCA AACAGCAAGA TTCGTTATGT AGCGATCATT CGGTAGAGCA GGCAAAACCT 
TCCACAAGCA AGACCACATC CAAAACCAAC GATAGAAAGA CTGAAGATGA AGTACTTTAC 
GCTCAACTTG CTCAAAATGC CATCCGAAAC CAACAACCAA GAAAGAACAA TACTGGAGTC 
CAAATAGCGC CAGAGTTGTC AGATATTGTC ATTTACATGC AAGCGACAAA ATTCAAAGGA 
TTTCCCCCAG TCGATGGCAT TCAATCACCA AGGATAATGG AGGAAGGGCC AGCTTCTGCA 
AGTCTTTCAT TTTCATCACG GGCTCGTACG CCATCAAATC TGCTGAATAC ACCAGCCCCT 
CCACGACGAC AGCGAAGTAG CACTCAGTTG AGTCAAGAAC TGGCTGCAGA ATTTTTGGGA 
TCAGTTAGGG CAAATGCTAC AGCTACATGC TACCAGGTTA CATCTCTTAA TGAAAACGCT 
GCAAAGAAAC TGATGAAAAG ACATCCAGCA AAATGCGTAT CTTATACTCG GGACCATCTG 
ATTCGTACGT ATCCAAGTGC CAAGCACTAT GACTCGTCCA ACTTTAACCC CATCAACTGC 
TGGGCTCATG GAATGCAAAT GGTTGCTTTA AATTTTCAAA CTCCTGATGT TATAATGGCA 
GTTAACCAAG CGATGTTCGA GCAATCTGGA AACTGTGGAT ATCAACTGAA ACCACGTTGT 
CTTTGGGATG AATCTCATCT TTTGTACAAC AAATTTTTGC CTCTCTCCAA AGACATTGCC 
GGCCATTCTG CTTTGTTACT AAACTTAACT ATTATATCCG GACAGCACGT GTATCCAAAC 
ACCCATTACG CCAGTTTATA TGTCGAAATT GAAGTCATCG GTATTCACAA CGACTGTGTT 
CGTGAGAAAT CAAAAGTGGT CCAGAGAAAT TCTGTGAATC CAATCTGGAA CCACACCACT 
CAACTTCGAA TAGCTTGTGT TGACTTAGCA TTCCTTCGCA TAGCAGTATG TGATAGTGGG 
CAAAATGGAA GAGTCGTTGC TCACCGAGTT GTTCCTGTCA AATGCATTCG TCCGGGTTTC 
CGACATCTCC CACTCAGAAC TCCAACTAAT CTTCCGATCG ACAACGCTAT GATATTTTTA 
AGAACAAGAT TCGAGCAAGA AGAACACATC TACCTCCACG ACGATGATTC AAACACCTAT 
TGTAATCTAG AACATACGCT GGCATACCGC ACAGACTTAA CACCCAATTT ATCTCCAACG 
CCAATTTTGA AGAAACAGAT CTTTGTTCTT CGAATTACAG GCGCGTTTGC TGATGAAACA 
GCAATTACAG TGCACAGCGA GTCAGGAAGT ACAGTGAAGA CGGTGATGCA ACAGGCGCTG 
CTTAACGCTG GAAAGAATGC GGATCAGGTT GAAGAGTATG TACTCATCGA AGAATCACTG 
CCGGCACCTT CTGGGGAAGA TCCAATTGAG CAGAGAGTAC TTCCTCTGAA CGAGCCTATC 
ATGGACGCCG TGGCCTGCTG GAACGGTTCA ATGCGTCGTT TTGTGTTACG AAAAAAAGGC 
AGTGACCCAT CATCTAGAGC TTGGATCACT TCAATTATAA AATCCGGAAC AAGTGGAAGC 
TCCACGTCAG TCTCACCAAG TCCGTTGACC AAAGATGGAC ACGTAAAGAG TGCATCAAGC 
AATCAACTTC ATGGACGATC ATTGGACACT GACGCTTTTG GAGAGCACCT TGAAGTGACA 
GAAGGGTTGA ACCCTCGTGC AAGATCAATG GGCGACACAT TTCTGGTATG TGTTCACAAT 
GTTAGTGAAG ATCAGCCTTA TGCAATCCTA CGTGCTGGAA TCCACAGCAC TGCCGCCGAC 
ATTATTCGTC AGGTGTTTGT TAAAGCTCGC CGTTCAAATG TAGACGATTC CGAGTTTGTT 
CTTGTTGAAG AAACCTGCGA TGATCCAAAA CTCAATCAAG GCCAAATGAC TCCAAAGTAT 
CCAAACAACC GCACCACAAG CCGAGTGTTA GGACAAAACG AAAATGTTTG GAAAGCACAA 
TCGAGGTGGA AGTCAATGGG CAGATTTGTA CTTGAAAACC GAAAGGACAC AGTGCACGCA 
ACCCTCGAAA AAGTGAGATC ATTTATATCA AAACTGGAAG CTGCAAAAGC AAGCATGGAT 
CCCAGACACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360635.1
CDS	1..10515
Translation	MSLRQASHKFPSVDSMPTSRDSSMETTDRYPFGYTTELARTEPSASTAKRLRQRLQRQQSGQSGLSGRRYTGESSECSSLVGTPTDTGRSFTFGAAQRVMRERDDRKPSVTQNLSEIMLEAIHYEDVNLVERLLTAHSSKQPMSTSPSIGSTNTLTDLAQRRHGAGAHRRSNASSTVSTHSGGRSTCAMMNTLHMAVAHKQRDIVELLLKNGYDPNTPASCHCKGNCTATGNIPLTSIIPRTHSMTPELCSTCSQLRVVSIVDQTPLGVAVRSQSSELIALLIAYGGDVNLGDEDGNTPLMLAVRESPLSWPCLHTLIFFGAQIEQKNMRGICPLDLAPELKKLQQTCVEELFKNACSGDCEPPSRSTPGPSAKNWNRLQVDNDPVIDCVSWEQAWELLKKMACNPECLDNIVNSLSKFATQMENTPSSVDRDQFDAHMGGLLHKMIHTAIEEYESSTPTYKKQKKLHLTWLLSQIATFCFTFLQKSGTSRQFSALNTLNKIIDAGLVHDLFAFNDVVFHSSRILNRCHAFDSDEPPTPSGESFNSNADQNSTVDHVFIYGAGYKDQSLLPSPTVAPTRHKSDLMNSFSEMDPSQVIVALHNAITMQNREVGSRSVCSPAHRWRQCCAHCTQILVARLLLFLTHAKKFRSRLSERHQLRSLVALLEPTLEPQLLCLLLQVLALIALDPSTHTTLIDIQIDDVLIQMLLPADDWYYTNHSTKYGTFVKYHAARVLVYVGMGDRVGSRVNLFNMMTFEPSAKSGSQQNEDDYICETCATPRSRSTFSRSAVSVEGVLLKVLAEVAELVRQSQHLSASEPITEESPSAGSPPAVLPNQAEQKSINEKNAEKLTQLGCQILISLEQLEAHLCKLGLVLDSVLLLRLLLHKLSWDLGLVTKKRVAVVDHVYKPITDPRAHSSCSLGNNRNFGKSKSFDRREDSKRDKNYLRVDQGSRASKRVHIRRSSSVEIIRPKRLSSGAKDIKNRERRKRLGTDTSSGSNRSKKTNSSSSSVQKHLPKYIQSLFRSRMGTDPCKRTSRSESTLPHRDSNESNTSGSDAVLEFTRKLQNYPLTRREAQRLTYRSNGQQDSNDSKMGPRTLGYVYLPELEIQGASPPRSPGPLTGNSLEEGTTMLLDTRRPSSPQAIPGLPLIEIRRPSALSQFEFGYFVNSPELSGSEVSDCAPLLLSGQIGGSRKSSDETSLGGWSSRASSVMSQRSSRSSVGLRLSTFSGGTSIASDNSGPFLFSFVLRKRASTIGTRIPIPRRALSRSSGDSLRVPDRESPLHLASMTEMNPDFQCVRQLILNLLNVYTKRNDNVVSTMKECADVLRQILNSPQHPTVKNWCAEIIHVVSTHVEEEEPTTAENNEQINDEYLEFQDQVISGSLPCPKEEAAYLASIQLSVEEQWPSNKRTQTIRRHLLKGQFGRIRDLAQKIMVTPWEVDQNLYCTPPRFPNESANASRAQSVVEEIQHRSRTPTLLRCITNTDGLMSEEMQAQCLPVDLRGDRRTIKLVKERKRKLFHSQVYESEIGMKKLYIQTAKKLAAFGCKVFQVKELLHGRTLRKTLRLLCLSSAQLCLLDGSTKLVLKRQHASTLQQWRVGGGVSKHQLLLEFRGTKWQLIAPSYNALKSISMTLWEIMQNSASNSIQRSLNQSMHRSHTEATVSTSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKVRSFISKLEAAKASMDPRHE

Target ORF information:

RefSeq Version	NM_001373492.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373492.1

ORF Insert Sequence:

ATGTCCTTAC GCCAAGCATC ACACAAATTT CCATCGGTGG ACTCAATGCC AACATCACGG 
GATTCATCGA TGGAAACAAC CGACCGGTAC CCGTTTGGGT ATACGACCGA ATTGGCACGT 
ACCGAACCAT CTGCATCAAC TGCCAAACGT CTTCGACAAA GGTTACAGCG ACAGCAAAGT 
GGTCAATCAG GATTATCCGG TCGACGGTAT ACTGGAGAAA GCAGCGAGTG CTCATCACTT 
GTCGGAACAC CCACAGACAC TGGCAGATCA TTTACATTTG GAGCAGCACA AAGAGTCATG 
CGGGAACGGG ATGACCGCAA ACCATCAGTT ACCCAGAACT TGTCAGAAAT AATGCTCGAG 
GCAATACATT ATGAAGATGT GAACTTGGTG GAAAGGTTGC TCACTGCGCA TAGTTCAAAA 
CAACCAATGT CAACATCTCC GTCAATTGGT AGCACAAACA CATTGACGGA TCTTGCTCAA 
CGTAGACACG GTGCGGGGGC ACACCGACGT TCCAATGCCA GTAGTACGGT GTCCACACAT 
TCCGGAGGAA GATCCACGTG TGCTATGATG AACACGTTGC ACATGGCTGT GGCTCATAAA 
CAACGAGATA TAGTGGAACT TTTGCTTAAA AACGGCTACG ATCCCAATAC TCCCGCATCG 
TGTCACTGTA AGGGAAATTG CACGGCGACT GGGAATATTC CACTGACTTC GATCATTCCG 
AGAACTCATT CAATGACGCC AGAATTGTGT TCGACGTGCT CTCAACTGAG GGTTGTGTCA 
ATCGTGGATC AAACACCGCT TGGTGTGGCA GTCAGATCAC AGTCCAGCGA ATTGATAGCA 
TTGTTGATTG CCTACGGAGG AGATGTTAAT TTAGGTGATG AAGATGGCAA CACGCCGTTG 
ATGCTAGCCG TACGAGAATC ACCTCTGTCT TGGCCTTGCC TGCACACGCT CATTTTCTTT 
GGAGCTCAAA TTGAACAAAA GAACATGCGC GGAATATGCC CTCTTGATCT TGCTCCAGAG 
CTGAAGAAAC TTCAACAAAC TTGCGTTGAA GAGCTTTTCA AAAATGCGTG CTCGGGTGAT 
TGTGAACCAC CATCTCGATC TACACCGGGA CCATCAGCCA AAAACTGGAA TAGACTACAA 
GTTGACAATG ATCCTGTAAT TGACTGCGTC AGTTGGGAAC AAGCATGGGA ATTGCTGAAG 
AAGATGGCAT GTAATCCAGA ATGTCTAGAT AACATCGTGA ATTCATTGTC AAAATTTGCA 
ACCCAAATGG AGAACACCCC GTCAAGCGTT GATCGTGATC AATTCGATGC ACACATGGGT 
GGCCTACTAC ATAAAATGAT TCATACAGCG ATTGAGGAGT ACGAGTCTTC GACACCAACC 
TACAAAAAGC AGAAGAAACT CCATCTCACC TGGCTGCTCA GCCAAATTGC CACGTTTTGT 
TTTACATTTC TGCAGAAAAG TGGAACGAGC CGACAATTCT CAGCACTGAA CACGTTGAAT 
AAAATTATTG ATGCTGGTTT AGTACATGAT TTGTTCGCAT TCAACGACGT TGTCTTTCAT 
TCTTCAAGAA TTTTGAACCG ATGCCATGCA TTTGATTCCG ACGAACCACC AACTCCATCT 
GGCGAATCTT TCAACTCAAA TGCAGACCAA AACTCAACTG TCGATCATGT TTTCATTTAC 
GGAGCTGGCT ATAAAGACCA ATCGTTATTA CCTTCTCCTA CGGTGGCACC AACTCGACAC 
AAGTCCGACT TAATGAATTC TTTCAGCGAA ATGGATCCAA GCCAGGTCAT TGTGGCCTTG 
CACAACGCGA TAACAATGCA AAACCGGGAA GTAGGGTCAA GATCTGTTTG TAGTCCGGCT 
CATCGATGGA GGCAGTGTTG TGCTCATTGT ACACAGATAT TGGTGGCCAG ATTATTGCTA 
TTCTTAACAC ACGCAAAAAA ATTTCGAAGT CGGTTGTCTG AACGACATCA GCTCAGAAGC 
TTGGTAGCTT TGTTAGAACC AACTTTGGAA CCTCAACTCT TATGTCTTTT ACTTCAAGTT 
CTAGCTCTTA TAGCGTTAGA TCCATCTACC CACACAACTT TGATAGACAT TCAAATTGAC 
GACGTCTTGA TTCAAATGCT ACTCCCAGCA GATGATTGGT ACTACACTAA TCACAGCACT 
AAATATGGAA CTTTTGTAAA GTACCATGCT GCTAGAGTTT TAGTGTACGT TGGAATGGGT 
GATCGAGTAG GAAGTCGAGT CAATCTGTTC AACATGATGA CGTTTGAGCC ATCTGCAAAA 
AGCGGGAGTC AACAAAACGA GGACGACTAC ATTTGTGAGA CATGTGCCAC ACCGCGCAGC 
CGAAGTACAT TTTCAAGATC AGCAGTTTCA GTGGAAGGTG TTCTGTTAAA AGTGTTGGCA 
GAAGTTGCTG AGCTTGTACG TCAGAGTCAA CACCTATCAG CAAGTGAACC AATAACTGAA 
GAATCGCCCA GTGCAGGATC TCCACCCGCT GTCCTACCAA ATCAGGCTGA ACAGAAAAGT 
ATTAACGAAA AAAATGCAGA GAAACTTACA CAACTTGGGT GCCAAATTTT GATCTCATTG 
GAACAACTTG AAGCTCATTT ATGCAAACTT GGACTGGTAC TTGATTCGGT TCTACTTCTC 
CGTCTACTTC TTCATAAGTT GTCATGGGAT CTTGGATTAG TCACAAAAAA GAGGGTTGCT 
GTGGTGGATC ATGTTTACAA ACCAATCACG GATCCGAGAG CTCATTCTAG CTGCAGTCTT 
GGAAACAACA GAAACTTTGG TAAATCTAAA TCTTTTGATA GGCGAGAAGA TAGCAAACGG 
GACAAAAATT ACTTGCGTGT TGATCAGGGA TCTCGTGCGT CAAAGAGAGT GCATATCAGA 
CGATCTAGTT CAGTAGAGAT TATCAGGCCA AAACGACTCA GTTCCGGAGC AAAAGATATC 
AAAAATCGAG AACGGAGGAA AAGGCTGGGA ACAGATACAA GTAGCGGGTC GAATCGTTCC 
AAGAAAACCA ACTCCTCGTC TTCATCTGTT CAGAAACACT TGCCAAAATA CATTCAATCA 
TTATTCCGAA GTCGGATGGG AACTGATCCA TGCAAGAGGA CATCGAGAAG TGAATCCACT 
TTACCGCACA GAGACTCTAA TGAGAGTAAT ACAAGTGGAA GTGATGCTGT TCTTGAGTTC 
ACAAGAAAAC TTCAAAATTA TCCGTTGACG AGACGCGAAG CGCAAAGATT GACGTATCGA 
AGCAATGGGC AACAAGATTC AAATGACTCG AAGATGGGAC CACGAACTTT AGGATACGTT 
TACCTCCCAG AACTAGAAAT TCAAGGTGCA AGTCCGCCAA GATCACCGGG ACCTCTAACT 
GGAAACTCGT TAGAAGAAGG AACCACAATG CTTTTGGACA CAAGACGGCC GAGCAGCCCA 
CAAGCAATCC CCGGCCTTCC TTTAATTGAA ATTCGTCGGC CCTCAGCTCT CTCTCAATTT 
GAATTTGGAT ACTTTGTAAA CTCACCAGAA TTATCAGGAA GTGAAGTATC TGATTGTGCC 
CCATTACTTC TCAGTGGTCA AATTGGTGGA AGCCGAAAAA GCTCCGACGA AACATCATTG 
GGAGGATGGT CCAGCCGTGC TTCATCGGTA ATGAGTCAAA GGTCGTCCAG ATCCAGTGTT 
GGCTTGAGGT TATCAACATT TTCTGGAGGG ACGAGTATTG CTTCGGATAA CTCTGGGCCA 
TTTTTGTTCA GTTTTGTTTT GAGAAAACGT GCATCTACTA TAGGAACTAG AATACCAATA 
CCTAGGAGAG CTTTGTCAAG ATCTTCAGGA GACAGTCTGA GAGTACCTGA TAGAGAAAGT 
CCACTTCATT TAGCAAGTAT GACAGAGATG AATCCGGATT TTCAATGTGT TCGACAGCTC 
ATCTTGAACC TTTTGAATGT TTACACGAAA AGAAATGACA ATGTGGTTTC TACAATGAAG 
GAATGTGCGG ATGTTCTTCG TCAAATCCTA AACTCTCCTC AACATCCAAC TGTCAAGAAT 
TGGTGTGCCG AAATAATTCA TGTAGTCAGT ACTCATGTAG AAGAAGAGGA ACCAACGACG 
GCAGAAAATA ATGAACAAAT TAATGACGAG TATCTGGAAT TTCAAGACCA AGTGATATCA 
GGAAGTTTAC CATGCCCGAA GGAAGAGGCT GCATACCTTG CAAGTATTCA GTTATCAGTT 
GAAGAGCAGT GGCCGAGTAA TAAAAGGACC CAGACAATTA GAAGACATTT ATTGAAAGGA 
CAGTTTGGTC GAATCCGGGA TCTCGCCCAA AAGATCATGG TGACTCCATG GGAAGTTGAT 
CAAAACTTGT ACTGCACACC ACCCAGATTT CCCAATGAAT CGGCAAATGC TAGTAGAGCA 
CAATCAGTGG TTGAGGAAAT TCAGCACAGA TCAAGAACTC CAACTCTTCT GCGATGTATC 
ACAAACACAG ACGGCTTAAT GTCAGAAGAG ATGCAAGCTC AGTGCCTTCC CGTCGATCTA 
CGTGGTGATC GTAGAACTAT CAAGCTGGTC AAGGAGAGGA AGCGAAAATT GTTCCATAGT 
CAAGTCTATG AAAGTGAAAT TGGCATGAAG AAATTGTACA TTCAGACCGC CAAAAAACTA 
GCCGCATTTG GTTGTAAGGT TTTTCAAGTT AAAGAGTTGC TTCACGGACG AACACTCAGA 
AAAACTCTTC GACTGCTTTG CCTTTCTTCT GCTCAATTGT GTCTTCTCGA CGGATCAACA 
AAACTTGTGC TTAAACGGCA GCACGCATCG ACGTTACAAC AGTGGAGAGT AGGTGGTGGA 
GTTAGTAAGC ATCAACTATT GCTCGAATTC CGTGGAACCA AGTGGCAACT TATTGCACCG 
TCGTATAATG CACTGAAATC GATATCGATG ACATTATGGG AGATTATGCA AAATTCTGCT 
AGCAATTCAA TCCAGCGGAG TCTCAACCAG TCAATGCATC GTTCGCACAC CGAGGCGACT 
GTCTCAACAT CACGTAGTGA CTCAACCTCT TCAACGCAAT CAGCCACAGC TGGATTCATT 
CTCAATGAAG AACCAATCAC ACTGTTCCGA CTTGAACTCG AACGACTTCA ATATATTCTT 
CATTTCCCTG AAGAAGTTGC ATTCCAACTA TCTTCTACTG AGTATCAGCT TTTCTACTCA 
ATACAACCCA TGGATTATGT TCGTTACGTC AGTTGCGATC TCACATCTGT TCCAGTGTCA 
GAAAATCCAA GTCCAGTGCG AAATCTTGTG AAAAGGCTAT CAGAGGTATC ATCCTGGATA 
ACACATGTTA TTGTCAGTCA GCCAACACAT GACGATCGAA AGGTTGCTCT TACTGCAATT 
TTGAGAATTG TGGAAACTTG CTGGAATATT GGAAACTTTA ATGCAGCTGT TGAGGTTCTT 
ATGGGTTTAA AGAGTGAAAA ACTCAGACCA TTCTGGCTTT CTTTGCGACA AGAGGAAAAG 
TCTCAGTTTG ATTCGCTTTG CGAAACCTTG CTTCCTGCAA ACCAAGCTCT CCCATCTCAA 
GCTTACATCA ACGCGGTCCA ACGAGCACTG AGAATGCCAC AATCTCGTGT GATTCCATTT 
TTTGGAATAT TTCTTCGTGA TCTCTACGCA ATTGTGAATG ACTTGCCGAA TATCGTTGTG 
ATTGGACAAG AAGGTGAAAC CCAAAAGCTG GAATTCATGA GCGATCCCAA TGGCGAAGAT 
CATTTTTCGT CGAGAATTGG AGTGGGTGGG CTGTTGAATG CTGATAAAAT CAACTTGGTG 
GCAATTGTTT TGGACAACTT GGAATTATTC CATCGTCACA GCAGGACAAT GATCAAATTG 
TTAGAAGAGC AAGCAGTCCC ACCAATTCAA ATTCCTCAGA ACGAGCGGGA GCAAAAAGAA 
AAGGAAGCAA AAACATATGA ACCAGTACAA GTGGTTCGCG GCTCTTCTCA TGGAGTGGCT 
CTCATCCCTC TTGATACCCT AACATTCGAT CTTGATGTTA TCCAACGACT CCAGCACGGA 
ACAACAGTGA TCCATTACGA ACCTGATTCG GGAAGAAGCA ATTTGTGTCT GCTGCGGTTA 
GACCCTAGTT GCGGTCAAAT AAACTGGCAT AAGATTTCAT ATTCAGTTAA CAAAGATCCC 
AAAGAAAAGG ATGTGCTCGC CAAAGTATCC GTTTCAAATC TGCAGCCGCT AGACTCTGGC 
CGAGGGGCTC CGTCACCAAT GCCATCTGGA AGAACACCAG GAACTGGAGG AGTTGGAGTA 
GAGGAGGGAG AACTCAAATT AAGTGTTGTG AAAGGAGTCG AACTAGTTGA TAGTTATGAT 
ATTGATATTG AGGCAATTTA CCGTCGTCAT TCTATGGAAG AGATGTCAGT TCCTGTTAGC 
TGTTGGAAAG TTAGTCATGG TCAGTTGTTA TCGGATAATG AGTTCATCTA CTTTTTGGCC 
CCTCAACAAA TTGCTCAATT TTGGACCAAC GGGTTGCAAT CTGTAGTTAA AAGTCTCCAA 
GGACAGCAAA GGTACCCTGA TCGGCGAATG TTATGGATCA AAAATGTCTA CTTATCACTT 
TACGAGATAA CGGGCGAATC AAACTGTGGA CCACGGCCAT TTGAAGCACT TCAGGCATTT 
GGGCTGAGTC AAACAAATAC TAACGCAACA CGACCCAATG ACTCGTCGCT GTCCAGTGAG 
CCAGGAGGAG CTAAAAGCAG ACTGAAGAAC CTTAAAAATG CTATGCAAAA GAAGCTTCGT 
GGTGCATCGC GCGAAGGAAG CAGATCTCAG TCACCTCAAC CTCATTCTCC TCTTGTCCGA 
CCACCATCAA TAAAATCGCA AATATCGTCA CAATCTGGTC CGCCGGGTCC GAACTCTCCA 
GGCTATTTGC TGAAACCACG TGGAGAACCT GCCAATAGTG ATGCAGGAGA CATTGATAGC 
ATTTATACAC CTCGATCAAG AACTCCAACA AGCAGCTCCT ATGGAGGGCG ATCCGTTGGA 
GGGAGATCAT GTAAATCGTG GAGATCAAGA GGAGGAGAGA CACCAAATTC GGGTTCCATA 
AGCAGCTCGG GTCAGATGTC AATTCAAGTG TCTGGTCTGA GTGGACCATC GGGAAAAGAG 
TTTCAGGAAA AACCATTAAC ACTTGTAGAG TTCGCTGAAC TTTTTCGATT GTTCAATACA 
AGAATGAGAA AAGATTTGAG AGATGTGTTC AATGATGTCC TGTCTACGGC AACTACTCCT 
CAGCATTGTC CTAAAAGAGA ACGTGATCGC CACAGTCCGA GAATGCAGTC AAGGCTGGCG 
AGTGTCTCCA ACTCGTACAA TGCAGATTTT CTGTCTAACG ATTTCTTGAC TCGGAATACG 
GCAGTAACGT CCCATCATAT CAGTGAAAAG CAAAACAAAA TCTACAATGC ATTGGCACTT 
GCGAGTGTTA ATAGCATGGG CGGACTCATG GACACTTCCC GGTCGTCGAT GCTGACGCCG 
CAAATGCTGC GCGCTTTTGT CAACACTCAT CAGATGGAGC AGATTGACGA GCAAACGGCG 
ATTAAGCTTA TTCAGGATCA TGAACCTGAT GGTATCTGTC GACAAAAGAA TCAAATGTCA 
TTCGAAGGAT TCACTAGATT CCTTTGCGAT CCTGTCAACT TTGCATTCGT ACCGGAAACA 
ATTGAACCAG ATGAAGAGGA CCTACGGTAC CCGTTGTCTC ATTATTACAT CAATTCAAGT 
CACAACACCT ATTTGACGGG ACATCAACTC AAGGGACCCA GTAGCTCGGA GATGTACAGA 
CAGGTTCTCC TCACCGGGTG TCGGTGTGTC GAGCTTGATT GTTGGGATGG AGATGACGGA 
TTGCCATTGA TTTATCATGG ACACACATTG GTTAGCAAGA TTGGGTTTAG ACAGGTGGTC 
GAAATTATCA AAAAATCTGC ATTCATAACA TCGGATCTTC CTGTGATATT ATCAATTGAG 
AATCATTGTT CCCTACAACA ACAGGCGAAA ATGGCACAGA TGTTCAAAAC AGTTCTTGGA 
GATTTACTAG TATCAAACTT CCTTTTTGAA GCTGATTTCT CGGACTCTCC ACGTCTTCCA 
TGCCCTCTCC AAATGAAAAA TAAGATCTTG ATAAAAAATA AGAAAATGAT AGTCGACCCA 
CCAACTCCAT TGCCAATGAT TGAACGAGGA GCTGTGCAGC GTGGTGAAAC TCAATTAAAT 
CTTCACAGAA AACAGAGTAA AAACTCCTAC GAAAGCAGTA CGGTAGATGA AGTGGAAGAT 
GACGACCTAG ATGAGTTTCT TGACGATGAA GAAAATGAGG AGGATGACCA AGAAGAGGTT 
CAAGTCAGAA GTGAAAAAGA AGACAGTCCG AAGACATCAA AACGTGCAGA GAAATCGGCT 
AGAAACATCA AACAGCAAGA TTCGTTATGT AGCGATCATT CGGTAGAGCA GGCAAAACCT 
TCCACAAGCA AGACCACATC CAAAACCAAC GATAGAAAGA CTGAAGATGA AGTACTTTAC 
GCTCAACTTG CTCAAAATGC CATCCGAAAC CAACAACCAA GAAAGAACAA TACTGGAGTC 
CAAATAGCGC CAGAGTTGTC AGATATTGTC ATTTACATGC AAGCGACAAA ATTCAAAGGA 
TTTCCCCCAG TCGATGGCAT TCAATCACCA AGGATAATGG AGGAAGGGCC AGCTTCTGCA 
AGTCTTTCAT TTTCATCACG GGCTCGTACG CCATCAAATC TGCTGAATAC ACCAGCCCCT 
CCACGACGAC AGCGAAGTAG CACTCAGTTG AGTCAAGAAC TGGCTGCAGA ATTTTTGGGA 
TCAGTTAGGG CAAATGCTAC AGCTACATGC TACCAGGTTA CATCTCTTAA TGAAAACGCT 
GCAAAGAAAC TGATGAAAAG ACATCCAGCA AAATGCGTAT CTTATACTCG GGACCATCTG 
ATTCGTACGT ATCCAAGTGC CAAGCACTAT GACTCGTCCA ACTTTAACCC CATCAACTGC 
TGGGCTCATG GAATGCAAAT GGTTGCTTTA AATTTTCAAA CTCCTGATGT TATAATGGCA 
GTTAACCAAG CGATGTTCGA GCAATCTGGA AACTGTGGAT ATCAACTGAA ACCACGTTGT 
CTTTGGGATG AATCTCATCT TTTGTACAAC AAATTTTTGC CTCTCTCCAA AGACATTGCC 
GGCCATTCTG CTTTGTTACT AAACTTAACT ATTATATCCG GACAGCACGT GTATCCAAAC 
ACCCATTACG CCAGTTTATA TGTCGAAATT GAAGTCATCG GTATTCACAA CGACTGTGTT 
CGTGAGAAAT CAAAAGTGGT CCAGAGAAAT TCTGTGAATC CAATCTGGAA CCACACCACT 
CAACTTCGAA TAGCTTGTGT TGACTTAGCA TTCCTTCGCA TAGCAGTATG TGATAGTGGG 
CAAAATGGAA GAGTCGTTGC TCACCGAGTT GTTCCTGTCA AATGCATTCG TCCGGGTTTC 
CGACATCTCC CACTCAGAAC TCCAACTAAT CTTCCGATCG ACAACGCTAT GATATTTTTA 
AGAACAAGAT TCGAGCAAGA AGAACACATC TACCTCCACG ACGATGATTC AAACACCTAT 
TGTAATCTAG AACATACGCT GGCATACCGC ACAGACTTAA CACCCAATTT ATCTCCAACG 
CCAATTTTGA AGAAACAGAT CTTTGTTCTT CGAATTACAG GCGCGTTTGC TGATGAAACA 
GCAATTACAG TGCACAGCGA GTCAGGAAGT ACAGTGAAGA CGGTGATGCA ACAGGCGCTG 
CTTAACGCTG GAAAGAATGC GGATCAGGTT GAAGAGTATG TACTCATCGA AGAATCACTG 
CCGGCACCTT CTGGGGAAGA TCCAATTGAG CAGAGAGTAC TTCCTCTGAA CGAGCCTATC 
ATGGACGCCG TGGCCTGCTG GAACGGTTCA ATGCGTCGTT TTGTGTTACG AAAAAAAGGC 
AGTGACCCAT CATCTAGAGC TTGGATCACT TCAATTATAA AATCCGGAAC AAGTGGAAGC 
TCCACGTCAG TCTCACCAAG TCCGTTGACC AAAGATGGAC ACGTAAAGAG TGCATCAAGC 
AATCAACTTC ATGGACGATC ATTGGACACT GACGCTTTTG GAGAGCACCT TGAAGTGACA 
GAAGGGTTGA ACCCTCGTGC AAGATCAATG GGCGACACAT TTCTGGTATG TGTTCACAAT 
GTTAGTGAAG ATCAGCCTTA TGCAATCCTA CGTGCTGGAA TCCACAGCAC TGCCGCCGAC 
ATTATTCGTC AGGTGTTTGT TAAAGCTCGC CGTTCAAATG TAGACGATTC CGAGTTTGTT 
CTTGTTGAAG AAACCTGCGA TGATCCAAAA CTCAATCAAG GCCAAATGAC TCCAAAGTAT 
CCAAACAACC GCACCACAAG CCGAGTGTTA GGACAAAACG AAAATGTTTG GAAAGCACAA 
TCGAGGTGGA AGTCAATGGG CAGATTTGTA CTTGAAAACC GAAAGGACAC AGTGCACGCA 
ACCCTCGAAA AAGTGAGATC ATTTATATCA AAACTGGAAG CTGCAAAAGC AAGCATGGAT 
CCCAGACACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_001373493.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360636.1 Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.

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CloneID	OCa851223
Clone ID Related Accession (Same CDS sequence)	NM_001373493.1
Accession Version	NM_001373493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	PhosphoLipase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on the 5' end.

ATGTCCTTAC GCCAAGCATC ACACAAATTT CCATCGGTGG ACTCAATGCC AACATCACGG 
GATTCATCGA TGGAAACAAC CGACCGGTAC CCGTTTGGGT ATACGACCGA ATTGGCACGT 
ACCGAACCAT CTGCATCAAC TGCCAAACGT CTTCGACAAA GGTTACAGCG ACAGCAAAGT 
GGTCAATCAG GATTATCCGG TCGACGGTAT ACTGGAGAAA GCAGCGAGTG CTCATCACTT 
GTCGGAACAC CCACAGACAC TGGCAGATCA TTTACATTTG GAGCAGCACA AAGAGTCATG 
CGGGAACGGG ATGACCGCAA ACCATCAGTT ACCCAGAACT TGTCAGAAAT AATGCTCGAG 
GCAATACATT ATGAAGATGT GAACTTGGTG GAAAGGTTGC TCACTGCGCA TAGTTCAAAA 
CAACCAATGT CAACATCTCC GTCAATTGGT AGCACAAACA CATTGACGGA TCTTGCTCAA 
CGTAGACACG GTGCGGGGGC ACACCGACGT TCCAATGCCA GTAGTACGGT GTCCACACAT 
TCCGGAGGAA GATCCACGTG TGCTATGATG AACACGTTGC ACATGGCTGT GGCTCATAAA 
CAACGAGATA TAGTGGAACT TTTGCTTAAA AACGGCTACG ATCCCAATAC TCCCGCATCG 
TGTCACTGTA AGGGAAATTG CACGGCGACT GGGAATATTC CACTGACTTC GATCATTCCG 
AGAACTCATT CAATGACGCC AGAATTGTGT TCGACGTGCT CTCAACTGAG GGTTGTGTCA 
ATCGTGGATC AAACACCGCT TGGTGTGGCA GTCAGATCAC AGTCCAGCGA ATTGATAGCA 
TTGTTGATTG CCTACGGAGG AGATGTTAAT TTAGGTGATG AAGATGGCAA CACGCCGTTG 
ATGCTAGCCG TACGAGAATC ACCTCTGTCT TGGCCTTGCC TGCACACGCT CATTTTCTTT 
GGAGCTCAAA TTGAACAAAA GAACATGCGC GGAATATGCC CTCTTGATCT TGCTCCAGAG 
CTGAAGAAAC TTCAACAAAC TTGCGTTGAA GAGCTTTTCA AAAATGCGTG CTCGGGTGAT 
TGTGAACCAC CATCTCGATC TACACCGGGA CCATCAGCCA AAAACTGGAA TAGACTACAA 
GTTGACAATG TTTCCCAAGG AGAACGATCA CTGTCAAAAG CTCCATTGAG CCCAAAACCG 
TCTGCTGCAG CAAGTGTCTC AACGTGTAGC ATGCTGGAAA CCTCATCAGC AAAAGAATGT 
GCACGACGAA AATCATTGGT GTCGCTTCAA CTTCACAGAA AAAGCCGAAA CGTGAAAGAA 
TATCCTGTAA TTGACTGCGT CAGTTGGGAA CAAGCATGGG AATTGCTGAA GAAGATGGCA 
TGTAATCCAG AATGTCTAGA TAACATCGTG AATTCATTGT CAAAATTTGC AACCCAAATG 
GAGAACACCC CGTCAAGCGT TGATCGTGAT CAATTCGATG CACACATGGG TGGCCTACTA 
CATAAAATGA TTCATACAGC GATTGAGGAG TACGAGTCTT CGACACCAAC CTACAAAAAG 
CAGAAGAAAC TCCATCTCAC CTGGCTGCTC AGCCAAATTG CCACGTTTTG TTTTACATTT 
CTGCAGAAAA GTGGAACGAG CCGACAATTC TCAGCACTGA ACACGTTGAA TAAAATTATT 
GATGCTGGTT TAGTACATGA TTTGTTCGCA TTCAACGACG TTGTCTTTCA TTCTTCAAGA 
ATTTTGAACC GATGCCATGC ATTTGATTCC GACGAACCAC CAACTCCATC TGGCGAATCT 
TTCAACTCAA ATGCAGACCA AAACTCAACT GTCGATCATG TTTTCATTTA CGGAGCTGGC 
TATAAAGACC AATCGTTATT ACCTTCTCCT ACGGTGGCAC CAACTCGACA CAAGTCCGAC 
TTAATGAATT CTTTCAGCGA AATGGATCCA AGCCAGGTCA TTGTGGCCTT GCACAACGCG 
ATAACAATGC AAAACCGGGA AGTAGGGTCA AGATCTGTTT GTAGTCCGGC TCATCGATGG 
AGGCAGTGTT GTGCTCATTG TACACAGATA TTGGTGGCCA GATTATTGCT ATTCTTAACA 
CACGCAAAAA AATTTCGAAG TCGGTTGTCT GAACGACATC AGCTCAGAAG CTTGGTAGCT 
TTGTTAGAAC CAACTTTGGA ACCTCAACTC TTATGTCTTT TACTTCAAGT TCTAGCTCTT 
ATAGCGTTAG ATCCATCTAC CCACACAACT TTGATAGACA TTCAAATTGA CGACGTCTTG 
ATTCAAATGC TACTCCCAGC AGATGATTGG TACTACACTA ATCACAGCAC TAAATATGGA 
ACTTTTGTAA AGTACCATGC TGCTAGAGTT TTAGTGTACG TTGGAATGGG TGATCGAGTA 
GGAAGTCGAG TCAATCTGTT CAACATGATG ACGTTTGAGC CATCTGCAAA AAGCGGGAGT 
CAACAAAACG AGGACGACTA CATTTGTGAG ACATGTGCCA CACCGCGCAG CCGAAGTACA 
TTTTCAAGAT CAGCAGTTTC AGTGGAAGGT GTTCTGTTAA AAGTGTTGGC AGAAGTTGCT 
GAGCTTGTAC GTCAGAGTCA ACACCTATCA GCAAGTGAAC CAATAACTGA AGAATCGCCC 
AGTGCAGGAT CTCCACCCGC TGTCCTACCA AATCAGGCTG AACAGAAAAG TATTAACGAA 
AAAAATGCAG AGAAACTTAC ACAACTTGGG TGCCAAATTT TGATCTCATT GGAACAACTT 
GAAGCTCATT TATGCAAACT TGGACTGGTA CTTGATTCGG TTCTACTTCT CCGTCTACTT 
CTTCATAAGT TGTCATGGGA TCTTGGATTA GTCACAAAAA AGAGGGTTGC TGTGGTGGAT 
CATGTTTACA AACCAATCAC GGATCCGAGA GCTCATTCTA GCTGCAGTCT TGGAAACAAC 
AGGCGAGAAG ATAGCAAACG GGACAAAAAT TACTTGCGTG TTGATCAGGG ATCTCGTGCG 
TCAAAGAGAG TGCATATCAG ACGATCTAGT TCAGTAGAGA TTATCAGGCC AAAACGACTC 
AGTTCCGGAG CAAAAGATAT CAAAAATCGA GAACGGAGGA AAAGGCTGGG AACAGATACA 
AGTAGCGGGT CGAATCGTTC CAAGAAAACC AACTCCTCGT CTTCATCTGT TCAGAAACAC 
TTGCCAAAAT ACATTCAATC ATTATTCCGA AGTCGGATGG GAACTGATCC ATGCAAGAGG 
ACATCGAGAA GAGACTCTAA TGAGAGTAAT ACAAGTGGAA GTGATGCTGT TCTTGAGTTC 
ACAAGAAAAC TTCAAAATTA TCCGTTGACG AGACGCGAAG CGCAAAGATT GACGTATCGA 
AGCAATGGGC AACAAGATTC AAATGACTCG AAGATGGGAC CACGAACTTT AGGATACGTT 
TACCTCCCAG AACTAGAAAT TCAAGGTGCA AGTCCGCCAA GATCACCGGG ACCTCTAACT 
GGAAACTCGT TAGAAGAAGG AACCACAATG CTTTTGGACA CAAGACGGCC GAGCAGCCCA 
CAAGCAATCC CCGGCCTTCC TTTAATTGAA ATTCGTCGGC CCTCAGCTCT CTCTCAATTT 
GAATTTGGAT ACTTTGTAAA CTCACCAGAA TTATCAGGAA GTGAAGTATC TGATTGTGCC 
CCATTACTTC TCAGTGGTCA AATTGGTGGA AGCCGAAAAA GCTCCGACGA AACATCATTG 
GGAGGATGGT CCAGCCGTGC TTCATCGGTA ATGAGTCAAA GGTCGTCCAG ATCCAGTGTT 
GGCTTGAGGT TATCAACATT TTCTGGAGGG ACGAGTATTG CTTCGGATAA CTCTGGGCCA 
TTTTTGTTCA GTTTTGTTTT GAGAAAACGT GCATCTACTA TAGGAACTAG AATACCAATA 
CCTAGGAGAG CTTTGTCAAG ATCTTCAGGA GACAGTCTGA GAGTACCTGA TAGAGAAAGT 
CCACTTCATT TAGCAAGTAT GACAGAGATG AATCCGGATT TTCAATGTGT TCGACAGCTC 
ATCTTGAACC TTTTGAATGT TTACACGAAA AGAAATGACA ATGTGGTTTC TACAATGAAG 
GAATGTGCGG ATGTTCTTCG TCAAATCCTA AACTCTCCTC AACATCCAAC TGTCAAGAAT 
TGGTGTGCCG AAATAATTCA TGTAGTCAGT ACTCATGTAG AAGAAGAGGA ACCAACGACG 
GCAGAAAATA ATGAACAAAT TAATGACGAG TATCTGGAAT TTCAAGACCA AGTGATATCA 
GGAAGTTTAC CATGCCCGAA GGAAGAGGCT GCATACCTTG CAAGTATTCA GTTATCAGTT 
GAAGAGCAGT GGCCGAGTAA TAAAAGGACC CAGACAATTA GAAGACATTT ATTGAAAGGA 
CAGTTTGGTC GAATCCGGGA TCTCGCCCAA AAGATCATGG TGACTCCATG GGAAGTTGAT 
CAAAACTTGT ACTGCACACC ACCCAGATTT CCCAATGAAT CGGCAAATGC TAGTAGAGCA 
CAATCAGTGG TTGAGGAAAT TCAGCACAGA TCAAGAACTC CAACTCTTCT GCGATGTATC 
ACAAACACAG ACGGCTTAAT GTCAGAAGAG ATGCAAGCTC AGTGCCTTCC CGTCGATCTA 
CGTGGTGATC GTAGAACTAT CAAGCTGGTC AAGGAGAGGA AGCGAAAATT GTTCCATAGT 
CAAGTCTATG AAAGTGAAAT TGGCATGAAG AAATTGTACA TTCAGACCGC CAAAAAACTA 
GCCGCATTTG GTTGTAAGGT TTTTCAAGTT AAAGAGTTGC TTCACGGACG AACACTCAGA 
AAAACTCTTC GACTGCTTTG CCTTTCTTCT GCTCAATTGT GTCTTCTCGA CGGATCAACA 
AAACTTGTGC TTAAACGGCA GCACGCATCG ACGTTACAAC AGTGGAGAGT AGGTGGTGGA 
GTTAGTAAGC ATCAACTATT GCTCGAATTC CGTGGAACCA AGTGGCAACT TATTGCACCG 
TCGTATAATG CACTGAAATC GATATCGATG ACATTATGGG AGATTATGCA AAATTCTGCT 
AGCAATTCAA TCCAGCGGAG TCTCAACCAG TCAATGCATC GTTCGCACAC CGAGGCGACT 
GTCTCAACAT CACGTAGTGA CTCAACCTCT TCAACGCAAT CAGCCACAGC TGGATTCATT 
CTCAATGAAG AACCAATCAC ACTGTTCCGA CTTGAACTCG AACGACTTCA ATATATTCTT 
CATTTCCCTG AAGAAGTTGC ATTCCAACTA TCTTCTACTG AGTATCAGCT TTTCTACTCA 
ATACAACCCA TGGATTATGT TCGTTACGTC AGTTGCGATC TCACATCTGT TCCAGTGTCA 
GAAAATCCAA GTCCAGTGCG AAATCTTGTG AAAAGGCTAT CAGAGGTATC ATCCTGGATA 
ACACATGTTA TTGTCAGTCA GCCAACACAT GACGATCGAA AGGTTGCTCT TACTGCAATT 
TTGAGAATTG TGGAAACTTG CTGGAATATT GGAAACTTTA ATGCAGCTGT TGAGGTTCTT 
ATGGGTTTAA AGAGTGAAAA ACTCAGACCA TTCTGGCTTT CTTTGCGACA AGAGGAAAAG 
TCTCAGTTTG ATTCGCTTTG CGAAACCTTG CTTCCTGCAA ACCAAGCTCT CCCATCTCAA 
GCTTACATCA ACGCGGTCCA ACGAGCACTG AGAATGCCAC AATCTCGTGT GATTCCATTT 
TTTGGAATAT TTCTTCGTGA TCTCTACGCA ATTGTGAATG ACTTGCCGAA TATCGTTGTG 
ATTGGACAAG AAGGTGAAAC CCAAAAGCTG GAATTCATGA GCGATCCCAA TGGCGAAGAT 
CATTTTTCGT CGAGAATTGG AGTGGGTGGG CTGTTGAATG CTGATAAAAT CAACTTGGTG 
GCAATTGTTT TGGACAACTT GGAATTATTC CATCGTCACA GCAGGACAAT GATCAAATTG 
TTAGAAGAGC AAGCAGTCCC ACCAATTCAA ATTCCTCAGA ACGAGCGGGA GCAAAAAGAA 
AAGGAAGCAA AAACATATGA ACCAGTACAA GTGGTTCGCG GCTCTTCTCA TGGAGTGGCT 
CTCATCCCTC TTGATACCCT AACATTCGAT CTTGATGTTA TCCAACGACT CCAGCACGGA 
ACAACAGTGA TCCATTACGA ACCTGATTCG GGAAGAAGCA ATTTGTGTCT GCTGCGGTTA 
GACCCTAGTT GCGGTCAAAT AAACTGGCAT AAGATTTCAT ATTCAGTTAA CAAAGATCCC 
AAAGAAAAGG ATGTGCTCGC CAAAGTATCC GTTTCAAATC TGCAGCCGCT AGACTCTGGC 
CGAGGGGCTC CGTCACCAAT GCCATCTGGA AGAACACCAG GAACTGGAGG AGTTGGAGTA 
GAGGAGGGAG AACTCAAATT AAGTGTTGTG AAAGGAGTCG AACTAGTTGA TAGTTATGAT 
ATTGATATTG AGGCAATTTA CCGTCGTCAT TCTATGGAAG AGATGTCAGT TCCTGTTAGC 
TGTTGGAAAG TTAGTCATGG TCAGTTGTTA TCGGATAATG AGTTCATCTA CTTTTTGGCC 
CCTCAACAAA TTGCTCAATT TTGGACCAAC GGGTTGCAAT CTGTAGTTAA AAGTCTCCAA 
GGACAGCAAA GGTACCCTGA TCGGCGAATG TTATGGATCA AAAATGTCTA CTTATCACTT 
TACGAGATAA CGGGCGAATC AAACTGTGGA CCACGGCCAT TTGAAGCACT TCAGGCATTT 
GGGCTGAGTC AAACAAATAC TAACGCAACA CGACCCAATG ACTCGTCGCT GTCCAGTGAG 
CCAGGAGGAG CTAAAAGCAG ACTGAAGAAC CTTAAAAATG CTATGCAAAA GAAGCTTCGT 
GGTGCATCGC GCGAAGGAAG CAGATCTCAG TCACCTCAAC CTCATTCTCC TCTTGTCCGA 
CCACCATCAA TAAAATCGCA AATATCGTCA CAATCTGGTC CGCCGGGTCC GAACTCTCCA 
GGCTATTTGC TGAAACCACG TGGAGAACCT GCCAATAGTG ATGCAGGAGA CATTGATAGC 
ATTTATACAC CTCGATCAAG AACTCCAACA AGCAGCTCCT ATGGAGGGCG ATCCGTTGGA 
GGGAGATCAT GTAAATCGTG GAGATCAAGA GGAGGAGAGA CACCAAATTC GGGTTCCATA 
AGCAGCTCGG GTCAGATGTC AATTCAAGTG TCTGGTCTGA GTGGACCATC GGGAAAAGAG 
TTTCAGGAAA AACCATTAAC ACTTGTAGAG TTCGCTGAAC TTTTTCGATT GTTCAATACA 
AGAATGAGAA AAGATTTGAG AGATGTGTTC AATGATGTCC TGTCTACGGC AACTACTCCT 
CAGCATTGTC CTAAAAGAGA ACGTGATCGC CACAGTCCGA GAATGCAGTC AAGGCTGGCG 
AGTGTCTCCA ACTCGTACAA TGCAGATTTT CTGTCTAACG ATTTCTTGAC TCGGAATACG 
GCAGTAACGT CCCATCATAT CAGTGAAAAG CAAAACAAAA TCTACAATGC ATTGGCACTT 
GCGAGTGTTA ATAGCATGGG CGGACTCATG GACACTTCCC GGTCGTCGAT GCTGACGCCG 
CAAATGCTGC GCGCTTTTGT CAACACTCAT CAGATGGAGC AGATTGACGA GCAAACGGCG 
ATTAAGCTTA TTCAGGATCA TGAACCTGAT GGTATCTGTC GACAAAAGAA TCAAATGTCA 
TTCGAAGGAT TCACTAGATT CCTTTGCGAT CCTGTCAACT TTGCATTCGT ACCGGAAACA 
ATTGAACCAG ATGAAGAGGA CCTACGGTAC CCGTTGTCTC ATTATTACAT CAATTCAAGT 
CACAACACCT ATTTGACGGG ACATCAACTC AAGGGACCCA GTAGCTCGGA GATGTACAGA 
CAGGTTCTCC TCACCGGGTG TCGGTGTGTC GAGCTTGATT GTTGGGATGG AGATGACGGA 
TTGCCATTGA TTTATCATGG ACACACATTG GTTAGCAAGA TTGGGTTTAG ACAGGTGGTC 
GAAATTATCA AAAAATCTGC ATTCATAACA TCGGATCTTC CTGTGATATT ATCAATTGAG 
AATCATTGTT CCCTACAACA ACAGGCGAAA ATGGCACAGA TGTTCAAAAC AGTTCTTGGA 
GATTTACTAG TATCAAACTT CCTTTTTGAA GCTGATTTCT CGGACTCTCC ACGTCTTCCA 
TGCCCTCTCC AAATGAAAAA TAAGATCTTG ATAAAAAATA AGAAAATGAT AGTCGACCCA 
CCAACTCCAT TGCCAATGAT TGAACGAGGA GCTGTGCAGC GTGGTGAAAC TCAATTAAAT 
CTTCACAGAA AACAGAGTAA AAACTCCTAC GAAAGCAGTA CGGTAGATGA AGTGGAAGAT 
GACGACCTAG ATGAGTTTCT TGACGATGAA GAAAATGAGG AGGATGACCA AGAAGAGGTT 
CAAGTCAGAA GTGAAAAAGA AGACAGTCCG AAGACATCAA AACGTGCAGA GAAATCGGCT 
AGAAACATCA AACAGCAAGA TTCGTTATGT AGCGATCATT CGGTAGAGCA GGCAAAACCT 
TCCACAAGCA AGACCACATC CAAAACCAAC GATAGAAAGA CTGAAGATGA AGTACTTTAC 
GCTCAACTTG CTCAAAATGC CATCCGAAAC CAACAACCAA GAAAGAACAA TACTGGAGTC 
CAAATAGCGC CAGAGTTGTC AGATATTGTC ATTTACATGC AAGCGACAAA ATTCAAAGGA 
TTTCCCCCAG TCGATGGCAT TCAATCACCA AGGATAATGG AGGAAGGGCC AGCTTCTGCA 
AGTCTTTCAT TTTCATCACG GGCTCGTACG CCATCAAATC TGCTGAATAC ACCAGCCCCT 
CCACGACGAC AGCGAAGTAG CACTCAGTTG AGTCAAGAAC TGGCTGCAGA ATTTTTGGGA 
TCAGTTAGGG CAAATGCTAC AGCTACATGC TACCAGGTTA CATCTCTTAA TGAAAACGCT 
GCAAAGAAAC TGATGAAAAG ACATCCAGCA AAATGCGTAT CTTATACTCG GGACCATCTG 
ATTCGTACGT ATCCAAGTGC CAAGCACTAT GACTCGTCCA ACTTTAACCC CATCAACTGC 
TGGGCTCATG GAATGCAAAT GGTTGCTTTA AATTTTCAAA CTCCTGATGT TATAATGGCA 
GTTAACCAAG CGATGTTCGA GCAATCTGGA AACTGTGGAT ATCAACTGAA ACCACGTTGT 
CTTTGGGATG AATCTCATCT TTTGTACAAC AAATTTTTGC CTCTCTCCAA AGACATTGCC 
GGCCATTCTG CTTTGTTACT AAACTTAACT ATTATATCCG GACAGCACGT GTATCCAAAC 
ACCCATTACG CCAGTTTATA TGTCGAAATT GAAGTCATCG GTATTCACAA CGACTGTGTT 
CGTGAGAAAT CAAAAGTGGT CCAGAGAAAT TCTGTGAATC CAATCTGGAA CCACACCACT 
CAACTTCGAA TAGCTTGTGT TGACTTAGCA TTCCTTCGCA TAGCAGTATG TGATAGTGGG 
CAAAATGGAA GAGTCGTTGC TCACCGAGTT GTTCCTGTCA AATGCATTCG TCCGGGTTTC 
CGACATCTCC CACTCAGAAC TCCAACTAAT CTTCCGATCG ACAACGCTAT GATATTTTTA 
AGAACAAGAT TCGAGCAAGA AGAACACATC TACCTCCACG ACGATGATTC AAACACCTAT 
TGTAATCTAG AACATACGCT GGCATACCGC ACAGACTTAA CACCCAATTT ATCTCCAACG 
CCAATTTTGA AGAAACAGAT CTTTGTTCTT CGAATTACAG GCGCGTTTGC TGATGAAACA 
GCAATTACAG TGCACAGCGA GTCAGGAAGT ACAGTGAAGA CGGTGATGCA ACAGGCGCTG 
CTTAACGCTG GAAAGAATGC GGATCAGGTT GAAGAGTATG TACTCATCGA AGAATCACTG 
CCGGCACCTT CTGGGGAAGA TCCAATTGAG CAGAGAGTAC TTCCTCTGAA CGAGCCTATC 
ATGGACGCCG TGGCCTGCTG GAACGGTTCA ATGCGTCGTT TTGTGTTACG AAAAAAAGGC 
AGTGACCCAT CATCTAGAGC TTGGATCACT TCAATTATAA AATCCGGAAC AAGTGGAAGC 
TCCACGTCAG TCTCACCAAG TCCGTTGACC AAAGATGGAC ACGTAAAGAG TGCATCAAGC 
AATCAACTTC ATGGACGATC ATTGGACACT GACGCTTTTG GAGAGCACCT TGAAGTGACA 
GAAGGGTTGA ACCCTCGTGC AAGATCAATG GGCGACACAT TTCTGGTATG TGTTCACAAT 
GTTAGTGAAG ATCAGCCTTA TGCAATCCTA CGTGCTGGAA TCCACAGCAC TGCCGCCGAC 
ATTATTCGTC AGGTGTTTGT TAAAGCTCGC CGTTCAAATG TAGACGATTC CGAGTTTGTT 
CTTGTTGAAG AAACCTGCGA TGATCCAAAA CTCAATCAAG GCCAAATGAC TCCAAAGTAT 
CCAAACAACC GCACCACAAG CCGAGTGTTA GGACAAAACG AAAATGTTTG GAAAGCACAA 
TCGAGGTGGA AGTCAATGGG CAGATTTGTA CTTGAAAACC GAAAGGACAC AGTGCACGCA 
ACCCTCGAAA AAGTGAGATC ATTTATATCA AAACTGGAAG CTGCAAAAGC AAGCATGGAT 
CCCAGACACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360636.1
CDS	1..10635
Translation	MSLRQASHKFPSVDSMPTSRDSSMETTDRYPFGYTTELARTEPSASTAKRLRQRLQRQQSGQSGLSGRRYTGESSECSSLVGTPTDTGRSFTFGAAQRVMRERDDRKPSVTQNLSEIMLEAIHYEDVNLVERLLTAHSSKQPMSTSPSIGSTNTLTDLAQRRHGAGAHRRSNASSTVSTHSGGRSTCAMMNTLHMAVAHKQRDIVELLLKNGYDPNTPASCHCKGNCTATGNIPLTSIIPRTHSMTPELCSTCSQLRVVSIVDQTPLGVAVRSQSSELIALLIAYGGDVNLGDEDGNTPLMLAVRESPLSWPCLHTLIFFGAQIEQKNMRGICPLDLAPELKKLQQTCVEELFKNACSGDCEPPSRSTPGPSAKNWNRLQVDNVSQGERSLSKAPLSPKPSAAASVSTCSMLETSSAKECARRKSLVSLQLHRKSRNVKEYPVIDCVSWEQAWELLKKMACNPECLDNIVNSLSKFATQMENTPSSVDRDQFDAHMGGLLHKMIHTAIEEYESSTPTYKKQKKLHLTWLLSQIATFCFTFLQKSGTSRQFSALNTLNKIIDAGLVHDLFAFNDVVFHSSRILNRCHAFDSDEPPTPSGESFNSNADQNSTVDHVFIYGAGYKDQSLLPSPTVAPTRHKSDLMNSFSEMDPSQVIVALHNAITMQNREVGSRSVCSPAHRWRQCCAHCTQILVARLLLFLTHAKKFRSRLSERHQLRSLVALLEPTLEPQLLCLLLQVLALIALDPSTHTTLIDIQIDDVLIQMLLPADDWYYTNHSTKYGTFVKYHAARVLVYVGMGDRVGSRVNLFNMMTFEPSAKSGSQQNEDDYICETCATPRSRSTFSRSAVSVEGVLLKVLAEVAELVRQSQHLSASEPITEESPSAGSPPAVLPNQAEQKSINEKNAEKLTQLGCQILISLEQLEAHLCKLGLVLDSVLLLRLLLHKLSWDLGLVTKKRVAVVDHVYKPITDPRAHSSCSLGNNRREDSKRDKNYLRVDQGSRASKRVHIRRSSSVEIIRPKRLSSGAKDIKNRERRKRLGTDTSSGSNRSKKTNSSSSSVQKHLPKYIQSLFRSRMGTDPCKRTSRRDSNESNTSGSDAVLEFTRKLQNYPLTRREAQRLTYRSNGQQDSNDSKMGPRTLGYVYLPELEIQGASPPRSPGPLTGNSLEEGTTMLLDTRRPSSPQAIPGLPLIEIRRPSALSQFEFGYFVNSPELSGSEVSDCAPLLLSGQIGGSRKSSDETSLGGWSSRASSVMSQRSSRSSVGLRLSTFSGGTSIASDNSGPFLFSFVLRKRASTIGTRIPIPRRALSRSSGDSLRVPDRESPLHLASMTEMNPDFQCVRQLILNLLNVYTKRNDNVVSTMKECADVLRQILNSPQHPTVKNWCAEIIHVVSTHVEEEEPTTAENNEQINDEYLEFQDQVISGSLPCPKEEAAYLASIQLSVEEQWPSNKRTQTIRRHLLKGQFGRIRDLAQKIMVTPWEVDQNLYCTPPRFPNESANASRAQSVVEEIQHRSRTPTLLRCITNTDGLMSEEMQAQCLPVDLRGDRRTIKLVKERKRKLFHSQVYESEIGMKKLYIQTAKKLAAFGCKVFQVKELLHGRTLRKTLRLLCLSSAQLCLLDGSTKLVLKRQHASTLQQWRVGGGVSKHQLLLEFRGTKWQLIAPSYNALKSISMTLWEIMQNSASNSIQRSLNQSMHRSHTEATVSTSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKVRSFISKLEAAKASMDPRHE

Target ORF information:

RefSeq Version	NM_001373493.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373493.1

ORF Insert Sequence:

ATGTCCTTAC GCCAAGCATC ACACAAATTT CCATCGGTGG ACTCAATGCC AACATCACGG 
GATTCATCGA TGGAAACAAC CGACCGGTAC CCGTTTGGGT ATACGACCGA ATTGGCACGT 
ACCGAACCAT CTGCATCAAC TGCCAAACGT CTTCGACAAA GGTTACAGCG ACAGCAAAGT 
GGTCAATCAG GATTATCCGG TCGACGGTAT ACTGGAGAAA GCAGCGAGTG CTCATCACTT 
GTCGGAACAC CCACAGACAC TGGCAGATCA TTTACATTTG GAGCAGCACA AAGAGTCATG 
CGGGAACGGG ATGACCGCAA ACCATCAGTT ACCCAGAACT TGTCAGAAAT AATGCTCGAG 
GCAATACATT ATGAAGATGT GAACTTGGTG GAAAGGTTGC TCACTGCGCA TAGTTCAAAA 
CAACCAATGT CAACATCTCC GTCAATTGGT AGCACAAACA CATTGACGGA TCTTGCTCAA 
CGTAGACACG GTGCGGGGGC ACACCGACGT TCCAATGCCA GTAGTACGGT GTCCACACAT 
TCCGGAGGAA GATCCACGTG TGCTATGATG AACACGTTGC ACATGGCTGT GGCTCATAAA 
CAACGAGATA TAGTGGAACT TTTGCTTAAA AACGGCTACG ATCCCAATAC TCCCGCATCG 
TGTCACTGTA AGGGAAATTG CACGGCGACT GGGAATATTC CACTGACTTC GATCATTCCG 
AGAACTCATT CAATGACGCC AGAATTGTGT TCGACGTGCT CTCAACTGAG GGTTGTGTCA 
ATCGTGGATC AAACACCGCT TGGTGTGGCA GTCAGATCAC AGTCCAGCGA ATTGATAGCA 
TTGTTGATTG CCTACGGAGG AGATGTTAAT TTAGGTGATG AAGATGGCAA CACGCCGTTG 
ATGCTAGCCG TACGAGAATC ACCTCTGTCT TGGCCTTGCC TGCACACGCT CATTTTCTTT 
GGAGCTCAAA TTGAACAAAA GAACATGCGC GGAATATGCC CTCTTGATCT TGCTCCAGAG 
CTGAAGAAAC TTCAACAAAC TTGCGTTGAA GAGCTTTTCA AAAATGCGTG CTCGGGTGAT 
TGTGAACCAC CATCTCGATC TACACCGGGA CCATCAGCCA AAAACTGGAA TAGACTACAA 
GTTGACAATG TTTCCCAAGG AGAACGATCA CTGTCAAAAG CTCCATTGAG CCCAAAACCG 
TCTGCTGCAG CAAGTGTCTC AACGTGTAGC ATGCTGGAAA CCTCATCAGC AAAAGAATGT 
GCACGACGAA AATCATTGGT GTCGCTTCAA CTTCACAGAA AAAGCCGAAA CGTGAAAGAA 
TATCCTGTAA TTGACTGCGT CAGTTGGGAA CAAGCATGGG AATTGCTGAA GAAGATGGCA 
TGTAATCCAG AATGTCTAGA TAACATCGTG AATTCATTGT CAAAATTTGC AACCCAAATG 
GAGAACACCC CGTCAAGCGT TGATCGTGAT CAATTCGATG CACACATGGG TGGCCTACTA 
CATAAAATGA TTCATACAGC GATTGAGGAG TACGAGTCTT CGACACCAAC CTACAAAAAG 
CAGAAGAAAC TCCATCTCAC CTGGCTGCTC AGCCAAATTG CCACGTTTTG TTTTACATTT 
CTGCAGAAAA GTGGAACGAG CCGACAATTC TCAGCACTGA ACACGTTGAA TAAAATTATT 
GATGCTGGTT TAGTACATGA TTTGTTCGCA TTCAACGACG TTGTCTTTCA TTCTTCAAGA 
ATTTTGAACC GATGCCATGC ATTTGATTCC GACGAACCAC CAACTCCATC TGGCGAATCT 
TTCAACTCAA ATGCAGACCA AAACTCAACT GTCGATCATG TTTTCATTTA CGGAGCTGGC 
TATAAAGACC AATCGTTATT ACCTTCTCCT ACGGTGGCAC CAACTCGACA CAAGTCCGAC 
TTAATGAATT CTTTCAGCGA AATGGATCCA AGCCAGGTCA TTGTGGCCTT GCACAACGCG 
ATAACAATGC AAAACCGGGA AGTAGGGTCA AGATCTGTTT GTAGTCCGGC TCATCGATGG 
AGGCAGTGTT GTGCTCATTG TACACAGATA TTGGTGGCCA GATTATTGCT ATTCTTAACA 
CACGCAAAAA AATTTCGAAG TCGGTTGTCT GAACGACATC AGCTCAGAAG CTTGGTAGCT 
TTGTTAGAAC CAACTTTGGA ACCTCAACTC TTATGTCTTT TACTTCAAGT TCTAGCTCTT 
ATAGCGTTAG ATCCATCTAC CCACACAACT TTGATAGACA TTCAAATTGA CGACGTCTTG 
ATTCAAATGC TACTCCCAGC AGATGATTGG TACTACACTA ATCACAGCAC TAAATATGGA 
ACTTTTGTAA AGTACCATGC TGCTAGAGTT TTAGTGTACG TTGGAATGGG TGATCGAGTA 
GGAAGTCGAG TCAATCTGTT CAACATGATG ACGTTTGAGC CATCTGCAAA AAGCGGGAGT 
CAACAAAACG AGGACGACTA CATTTGTGAG ACATGTGCCA CACCGCGCAG CCGAAGTACA 
TTTTCAAGAT CAGCAGTTTC AGTGGAAGGT GTTCTGTTAA AAGTGTTGGC AGAAGTTGCT 
GAGCTTGTAC GTCAGAGTCA ACACCTATCA GCAAGTGAAC CAATAACTGA AGAATCGCCC 
AGTGCAGGAT CTCCACCCGC TGTCCTACCA AATCAGGCTG AACAGAAAAG TATTAACGAA 
AAAAATGCAG AGAAACTTAC ACAACTTGGG TGCCAAATTT TGATCTCATT GGAACAACTT 
GAAGCTCATT TATGCAAACT TGGACTGGTA CTTGATTCGG TTCTACTTCT CCGTCTACTT 
CTTCATAAGT TGTCATGGGA TCTTGGATTA GTCACAAAAA AGAGGGTTGC TGTGGTGGAT 
CATGTTTACA AACCAATCAC GGATCCGAGA GCTCATTCTA GCTGCAGTCT TGGAAACAAC 
AGGCGAGAAG ATAGCAAACG GGACAAAAAT TACTTGCGTG TTGATCAGGG ATCTCGTGCG 
TCAAAGAGAG TGCATATCAG ACGATCTAGT TCAGTAGAGA TTATCAGGCC AAAACGACTC 
AGTTCCGGAG CAAAAGATAT CAAAAATCGA GAACGGAGGA AAAGGCTGGG AACAGATACA 
AGTAGCGGGT CGAATCGTTC CAAGAAAACC AACTCCTCGT CTTCATCTGT TCAGAAACAC 
TTGCCAAAAT ACATTCAATC ATTATTCCGA AGTCGGATGG GAACTGATCC ATGCAAGAGG 
ACATCGAGAA GAGACTCTAA TGAGAGTAAT ACAAGTGGAA GTGATGCTGT TCTTGAGTTC 
ACAAGAAAAC TTCAAAATTA TCCGTTGACG AGACGCGAAG CGCAAAGATT GACGTATCGA 
AGCAATGGGC AACAAGATTC AAATGACTCG AAGATGGGAC CACGAACTTT AGGATACGTT 
TACCTCCCAG AACTAGAAAT TCAAGGTGCA AGTCCGCCAA GATCACCGGG ACCTCTAACT 
GGAAACTCGT TAGAAGAAGG AACCACAATG CTTTTGGACA CAAGACGGCC GAGCAGCCCA 
CAAGCAATCC CCGGCCTTCC TTTAATTGAA ATTCGTCGGC CCTCAGCTCT CTCTCAATTT 
GAATTTGGAT ACTTTGTAAA CTCACCAGAA TTATCAGGAA GTGAAGTATC TGATTGTGCC 
CCATTACTTC TCAGTGGTCA AATTGGTGGA AGCCGAAAAA GCTCCGACGA AACATCATTG 
GGAGGATGGT CCAGCCGTGC TTCATCGGTA ATGAGTCAAA GGTCGTCCAG ATCCAGTGTT 
GGCTTGAGGT TATCAACATT TTCTGGAGGG ACGAGTATTG CTTCGGATAA CTCTGGGCCA 
TTTTTGTTCA GTTTTGTTTT GAGAAAACGT GCATCTACTA TAGGAACTAG AATACCAATA 
CCTAGGAGAG CTTTGTCAAG ATCTTCAGGA GACAGTCTGA GAGTACCTGA TAGAGAAAGT 
CCACTTCATT TAGCAAGTAT GACAGAGATG AATCCGGATT TTCAATGTGT TCGACAGCTC 
ATCTTGAACC TTTTGAATGT TTACACGAAA AGAAATGACA ATGTGGTTTC TACAATGAAG 
GAATGTGCGG ATGTTCTTCG TCAAATCCTA AACTCTCCTC AACATCCAAC TGTCAAGAAT 
TGGTGTGCCG AAATAATTCA TGTAGTCAGT ACTCATGTAG AAGAAGAGGA ACCAACGACG 
GCAGAAAATA ATGAACAAAT TAATGACGAG TATCTGGAAT TTCAAGACCA AGTGATATCA 
GGAAGTTTAC CATGCCCGAA GGAAGAGGCT GCATACCTTG CAAGTATTCA GTTATCAGTT 
GAAGAGCAGT GGCCGAGTAA TAAAAGGACC CAGACAATTA GAAGACATTT ATTGAAAGGA 
CAGTTTGGTC GAATCCGGGA TCTCGCCCAA AAGATCATGG TGACTCCATG GGAAGTTGAT 
CAAAACTTGT ACTGCACACC ACCCAGATTT CCCAATGAAT CGGCAAATGC TAGTAGAGCA 
CAATCAGTGG TTGAGGAAAT TCAGCACAGA TCAAGAACTC CAACTCTTCT GCGATGTATC 
ACAAACACAG ACGGCTTAAT GTCAGAAGAG ATGCAAGCTC AGTGCCTTCC CGTCGATCTA 
CGTGGTGATC GTAGAACTAT CAAGCTGGTC AAGGAGAGGA AGCGAAAATT GTTCCATAGT 
CAAGTCTATG AAAGTGAAAT TGGCATGAAG AAATTGTACA TTCAGACCGC CAAAAAACTA 
GCCGCATTTG GTTGTAAGGT TTTTCAAGTT AAAGAGTTGC TTCACGGACG AACACTCAGA 
AAAACTCTTC GACTGCTTTG CCTTTCTTCT GCTCAATTGT GTCTTCTCGA CGGATCAACA 
AAACTTGTGC TTAAACGGCA GCACGCATCG ACGTTACAAC AGTGGAGAGT AGGTGGTGGA 
GTTAGTAAGC ATCAACTATT GCTCGAATTC CGTGGAACCA AGTGGCAACT TATTGCACCG 
TCGTATAATG CACTGAAATC GATATCGATG ACATTATGGG AGATTATGCA AAATTCTGCT 
AGCAATTCAA TCCAGCGGAG TCTCAACCAG TCAATGCATC GTTCGCACAC CGAGGCGACT 
GTCTCAACAT CACGTAGTGA CTCAACCTCT TCAACGCAAT CAGCCACAGC TGGATTCATT 
CTCAATGAAG AACCAATCAC ACTGTTCCGA CTTGAACTCG AACGACTTCA ATATATTCTT 
CATTTCCCTG AAGAAGTTGC ATTCCAACTA TCTTCTACTG AGTATCAGCT TTTCTACTCA 
ATACAACCCA TGGATTATGT TCGTTACGTC AGTTGCGATC TCACATCTGT TCCAGTGTCA 
GAAAATCCAA GTCCAGTGCG AAATCTTGTG AAAAGGCTAT CAGAGGTATC ATCCTGGATA 
ACACATGTTA TTGTCAGTCA GCCAACACAT GACGATCGAA AGGTTGCTCT TACTGCAATT 
TTGAGAATTG TGGAAACTTG CTGGAATATT GGAAACTTTA ATGCAGCTGT TGAGGTTCTT 
ATGGGTTTAA AGAGTGAAAA ACTCAGACCA TTCTGGCTTT CTTTGCGACA AGAGGAAAAG 
TCTCAGTTTG ATTCGCTTTG CGAAACCTTG CTTCCTGCAA ACCAAGCTCT CCCATCTCAA 
GCTTACATCA ACGCGGTCCA ACGAGCACTG AGAATGCCAC AATCTCGTGT GATTCCATTT 
TTTGGAATAT TTCTTCGTGA TCTCTACGCA ATTGTGAATG ACTTGCCGAA TATCGTTGTG 
ATTGGACAAG AAGGTGAAAC CCAAAAGCTG GAATTCATGA GCGATCCCAA TGGCGAAGAT 
CATTTTTCGT CGAGAATTGG AGTGGGTGGG CTGTTGAATG CTGATAAAAT CAACTTGGTG 
GCAATTGTTT TGGACAACTT GGAATTATTC CATCGTCACA GCAGGACAAT GATCAAATTG 
TTAGAAGAGC AAGCAGTCCC ACCAATTCAA ATTCCTCAGA ACGAGCGGGA GCAAAAAGAA 
AAGGAAGCAA AAACATATGA ACCAGTACAA GTGGTTCGCG GCTCTTCTCA TGGAGTGGCT 
CTCATCCCTC TTGATACCCT AACATTCGAT CTTGATGTTA TCCAACGACT CCAGCACGGA 
ACAACAGTGA TCCATTACGA ACCTGATTCG GGAAGAAGCA ATTTGTGTCT GCTGCGGTTA 
GACCCTAGTT GCGGTCAAAT AAACTGGCAT AAGATTTCAT ATTCAGTTAA CAAAGATCCC 
AAAGAAAAGG ATGTGCTCGC CAAAGTATCC GTTTCAAATC TGCAGCCGCT AGACTCTGGC 
CGAGGGGCTC CGTCACCAAT GCCATCTGGA AGAACACCAG GAACTGGAGG AGTTGGAGTA 
GAGGAGGGAG AACTCAAATT AAGTGTTGTG AAAGGAGTCG AACTAGTTGA TAGTTATGAT 
ATTGATATTG AGGCAATTTA CCGTCGTCAT TCTATGGAAG AGATGTCAGT TCCTGTTAGC 
TGTTGGAAAG TTAGTCATGG TCAGTTGTTA TCGGATAATG AGTTCATCTA CTTTTTGGCC 
CCTCAACAAA TTGCTCAATT TTGGACCAAC GGGTTGCAAT CTGTAGTTAA AAGTCTCCAA 
GGACAGCAAA GGTACCCTGA TCGGCGAATG TTATGGATCA AAAATGTCTA CTTATCACTT 
TACGAGATAA CGGGCGAATC AAACTGTGGA CCACGGCCAT TTGAAGCACT TCAGGCATTT 
GGGCTGAGTC AAACAAATAC TAACGCAACA CGACCCAATG ACTCGTCGCT GTCCAGTGAG 
CCAGGAGGAG CTAAAAGCAG ACTGAAGAAC CTTAAAAATG CTATGCAAAA GAAGCTTCGT 
GGTGCATCGC GCGAAGGAAG CAGATCTCAG TCACCTCAAC CTCATTCTCC TCTTGTCCGA 
CCACCATCAA TAAAATCGCA AATATCGTCA CAATCTGGTC CGCCGGGTCC GAACTCTCCA 
GGCTATTTGC TGAAACCACG TGGAGAACCT GCCAATAGTG ATGCAGGAGA CATTGATAGC 
ATTTATACAC CTCGATCAAG AACTCCAACA AGCAGCTCCT ATGGAGGGCG ATCCGTTGGA 
GGGAGATCAT GTAAATCGTG GAGATCAAGA GGAGGAGAGA CACCAAATTC GGGTTCCATA 
AGCAGCTCGG GTCAGATGTC AATTCAAGTG TCTGGTCTGA GTGGACCATC GGGAAAAGAG 
TTTCAGGAAA AACCATTAAC ACTTGTAGAG TTCGCTGAAC TTTTTCGATT GTTCAATACA 
AGAATGAGAA AAGATTTGAG AGATGTGTTC AATGATGTCC TGTCTACGGC AACTACTCCT 
CAGCATTGTC CTAAAAGAGA ACGTGATCGC CACAGTCCGA GAATGCAGTC AAGGCTGGCG 
AGTGTCTCCA ACTCGTACAA TGCAGATTTT CTGTCTAACG ATTTCTTGAC TCGGAATACG 
GCAGTAACGT CCCATCATAT CAGTGAAAAG CAAAACAAAA TCTACAATGC ATTGGCACTT 
GCGAGTGTTA ATAGCATGGG CGGACTCATG GACACTTCCC GGTCGTCGAT GCTGACGCCG 
CAAATGCTGC GCGCTTTTGT CAACACTCAT CAGATGGAGC AGATTGACGA GCAAACGGCG 
ATTAAGCTTA TTCAGGATCA TGAACCTGAT GGTATCTGTC GACAAAAGAA TCAAATGTCA 
TTCGAAGGAT TCACTAGATT CCTTTGCGAT CCTGTCAACT TTGCATTCGT ACCGGAAACA 
ATTGAACCAG ATGAAGAGGA CCTACGGTAC CCGTTGTCTC ATTATTACAT CAATTCAAGT 
CACAACACCT ATTTGACGGG ACATCAACTC AAGGGACCCA GTAGCTCGGA GATGTACAGA 
CAGGTTCTCC TCACCGGGTG TCGGTGTGTC GAGCTTGATT GTTGGGATGG AGATGACGGA 
TTGCCATTGA TTTATCATGG ACACACATTG GTTAGCAAGA TTGGGTTTAG ACAGGTGGTC 
GAAATTATCA AAAAATCTGC ATTCATAACA TCGGATCTTC CTGTGATATT ATCAATTGAG 
AATCATTGTT CCCTACAACA ACAGGCGAAA ATGGCACAGA TGTTCAAAAC AGTTCTTGGA 
GATTTACTAG TATCAAACTT CCTTTTTGAA GCTGATTTCT CGGACTCTCC ACGTCTTCCA 
TGCCCTCTCC AAATGAAAAA TAAGATCTTG ATAAAAAATA AGAAAATGAT AGTCGACCCA 
CCAACTCCAT TGCCAATGAT TGAACGAGGA GCTGTGCAGC GTGGTGAAAC TCAATTAAAT 
CTTCACAGAA AACAGAGTAA AAACTCCTAC GAAAGCAGTA CGGTAGATGA AGTGGAAGAT 
GACGACCTAG ATGAGTTTCT TGACGATGAA GAAAATGAGG AGGATGACCA AGAAGAGGTT 
CAAGTCAGAA GTGAAAAAGA AGACAGTCCG AAGACATCAA AACGTGCAGA GAAATCGGCT 
AGAAACATCA AACAGCAAGA TTCGTTATGT AGCGATCATT CGGTAGAGCA GGCAAAACCT 
TCCACAAGCA AGACCACATC CAAAACCAAC GATAGAAAGA CTGAAGATGA AGTACTTTAC 
GCTCAACTTG CTCAAAATGC CATCCGAAAC CAACAACCAA GAAAGAACAA TACTGGAGTC 
CAAATAGCGC CAGAGTTGTC AGATATTGTC ATTTACATGC AAGCGACAAA ATTCAAAGGA 
TTTCCCCCAG TCGATGGCAT TCAATCACCA AGGATAATGG AGGAAGGGCC AGCTTCTGCA 
AGTCTTTCAT TTTCATCACG GGCTCGTACG CCATCAAATC TGCTGAATAC ACCAGCCCCT 
CCACGACGAC AGCGAAGTAG CACTCAGTTG AGTCAAGAAC TGGCTGCAGA ATTTTTGGGA 
TCAGTTAGGG CAAATGCTAC AGCTACATGC TACCAGGTTA CATCTCTTAA TGAAAACGCT 
GCAAAGAAAC TGATGAAAAG ACATCCAGCA AAATGCGTAT CTTATACTCG GGACCATCTG 
ATTCGTACGT ATCCAAGTGC CAAGCACTAT GACTCGTCCA ACTTTAACCC CATCAACTGC 
TGGGCTCATG GAATGCAAAT GGTTGCTTTA AATTTTCAAA CTCCTGATGT TATAATGGCA 
GTTAACCAAG CGATGTTCGA GCAATCTGGA AACTGTGGAT ATCAACTGAA ACCACGTTGT 
CTTTGGGATG AATCTCATCT TTTGTACAAC AAATTTTTGC CTCTCTCCAA AGACATTGCC 
GGCCATTCTG CTTTGTTACT AAACTTAACT ATTATATCCG GACAGCACGT GTATCCAAAC 
ACCCATTACG CCAGTTTATA TGTCGAAATT GAAGTCATCG GTATTCACAA CGACTGTGTT 
CGTGAGAAAT CAAAAGTGGT CCAGAGAAAT TCTGTGAATC CAATCTGGAA CCACACCACT 
CAACTTCGAA TAGCTTGTGT TGACTTAGCA TTCCTTCGCA TAGCAGTATG TGATAGTGGG 
CAAAATGGAA GAGTCGTTGC TCACCGAGTT GTTCCTGTCA AATGCATTCG TCCGGGTTTC 
CGACATCTCC CACTCAGAAC TCCAACTAAT CTTCCGATCG ACAACGCTAT GATATTTTTA 
AGAACAAGAT TCGAGCAAGA AGAACACATC TACCTCCACG ACGATGATTC AAACACCTAT 
TGTAATCTAG AACATACGCT GGCATACCGC ACAGACTTAA CACCCAATTT ATCTCCAACG 
CCAATTTTGA AGAAACAGAT CTTTGTTCTT CGAATTACAG GCGCGTTTGC TGATGAAACA 
GCAATTACAG TGCACAGCGA GTCAGGAAGT ACAGTGAAGA CGGTGATGCA ACAGGCGCTG 
CTTAACGCTG GAAAGAATGC GGATCAGGTT GAAGAGTATG TACTCATCGA AGAATCACTG 
CCGGCACCTT CTGGGGAAGA TCCAATTGAG CAGAGAGTAC TTCCTCTGAA CGAGCCTATC 
ATGGACGCCG TGGCCTGCTG GAACGGTTCA ATGCGTCGTT TTGTGTTACG AAAAAAAGGC 
AGTGACCCAT CATCTAGAGC TTGGATCACT TCAATTATAA AATCCGGAAC AAGTGGAAGC 
TCCACGTCAG TCTCACCAAG TCCGTTGACC AAAGATGGAC ACGTAAAGAG TGCATCAAGC 
AATCAACTTC ATGGACGATC ATTGGACACT GACGCTTTTG GAGAGCACCT TGAAGTGACA 
GAAGGGTTGA ACCCTCGTGC AAGATCAATG GGCGACACAT TTCTGGTATG TGTTCACAAT 
GTTAGTGAAG ATCAGCCTTA TGCAATCCTA CGTGCTGGAA TCCACAGCAC TGCCGCCGAC 
ATTATTCGTC AGGTGTTTGT TAAAGCTCGC CGTTCAAATG TAGACGATTC CGAGTTTGTT 
CTTGTTGAAG AAACCTGCGA TGATCCAAAA CTCAATCAAG GCCAAATGAC TCCAAAGTAT 
CCAAACAACC GCACCACAAG CCGAGTGTTA GGACAAAACG AAAATGTTTG GAAAGCACAA 
TCGAGGTGGA AGTCAATGGG CAGATTTGTA CTTGAAAACC GAAAGGACAC AGTGCACGCA 
ACCCTCGAAA AAGTGAGATC ATTTATATCA AAACTGGAAG CTGCAAAAGC AAGCATGGAT 
CCCAGACACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_001373489.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360637.1 Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.

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CloneID	OCa851224
Clone ID Related Accession (Same CDS sequence)	NM_001373489.1
Accession Version	NM_001373489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	PhosphoLipase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on the 5' end.

ATGTCCTTAC GCCAAGCATC ACACAAATTT CCATCGGTGG ACTCAATGCC AACATCACGG 
GATTCATCGA TGGAAACAAC CGACCGGTAC CCGTTTGGGT ATACGACCGA ATTGGCACGT 
ACCGAACCAT CTGCATCAAC TGCCAAACGT CTTCGACAAA GGTTACAGCG ACAGCAAAGT 
GGTCAATCAG GATTATCCGG TCGACGGTAT ACTGGAGAAA GCAGCGAGTG CTCATCACTT 
GTCGGAACAC CCACAGACAC TGGCAGATCA TTTACATTTG GAGCAGCACA AAGAGTCATG 
CGGGAACGGG ATGACCGCAA ACCATCAGTT ACCCAGAACT TGTCAGAAAT AATGCTCGAG 
GCAATACATT ATGAAGATGT GAACTTGGTG GAAAGGTTGC TCACTGCGCA TAGTTCAAAA 
CAACCAATGT CAACATCTCC GTCAATTGGT AGCACAAACA CATTGACGGA TCTTGCTCAA 
CGTAGACACG GTGCGGGGGC ACACCGACGT TCCAATGCCA GTAGTACGGT GTCCACACAT 
TCCGGAGGAA GATCCACGTG TGCTATGATG AACACGTTGC ACATGGCTGT GGCTCATAAA 
CAACGAGATA TAGTGGAACT TTTGCTTAAA AACGGCTACG ATCCCAATAC TCCCGCATCG 
TGTCACTGTA AGGGAAATTG CACGGCGACT GGGAATATTC CACTGACTTC GATCATTCCG 
AGAACTCATT CAATGACGCC AGAATTGTGT TCGACGTGCT CTCAACTGAG GGTTGTGTCA 
ATCGTGGATC AAACACCGCT TGGTGTGGCA GTCAGATCAC AGTCCAGCGA ATTGATAGCA 
TTGTTGATTG CCTACGGAGG AGATGTTAAT TTAGGTGATG AAGATGGCAA CACGCCGTTG 
ATGCTAGCCG TACGAGAATC ACCTCTGTCT TGGCCTTGCC TGCACACGCT CATTTTCTTT 
GGAGCTCAAA TTGAACAAAA GAACATGCGC GGAATATGCC CTCTTGATCT TGCTCCAGAG 
CTGAAGAAAC TTCAACAAAC TTGCGTTGAA GAGCTTTTCA AAAATGCGTG CTCGGGTGAT 
TGTGAACCAC CATCTCGATC TACACCGGGA CCATCAGCCA AAAACTGGAA TAGACTACAA 
GTTGACAATG ATCCTGTAAT TGACTGCGTC AGTTGGGAAC AAGCATGGGA ATTGCTGAAG 
AAGATGGCAT GTAATCCAGA ATGTCTAGAT AACATCGTGA ATTCATTGTC AAAATTTGCA 
ACCCAAATGG AGAACACCCC GTCAAGCGTT GATCGTGATC AATTCGATGC ACACATGGGT 
GGCCTACTAC ATAAAATGAT TCATACAGCG ATTGAGGAGT ACGAGTCTTC GACACCAACC 
TACAAAAAGC AGAAGAAACT CCATCTCACC TGGCTGCTCA GCCAAATTGC CACGTTTTGT 
TTTACATTTC TGCAGAAAAG TGGAACGAGC CGACAATTCT CAGCACTGAA CACGTTGAAT 
AAAATTATTG ATGCTGGTTT AGTACATGAT TTGTTCGCAT TCAACGACGT TGTCTTTCAT 
TCTTCAAGAA TTTTGAACCG ATGCCATGCA TTTGATTCCG ACGAACCACC AACTCCATCT 
GGCGAATCTT TCAACTCAAA TGCAGACCAA AACTCAACTG TCGATCATGT TTTCATTTAC 
GGAGCTGGCT ATAAAGACCA ATCGTTATTA CCTTCTCCTA CGGTGGCACC AACTCGACAC 
AAGTCCGACT TAATGAATTC TTTCAGCGAA ATGGATCCAA GCCAGGTCAT TGTGGCCTTG 
CACAACGCGA TAACAATGCA AAACCGGGAA GTAGGGTCAA GATCTGTTTG TAGTCCGGCT 
CATCGATGGA GGCAGTGTTG TGCTCATTGT ACACAGATAT TGGTGGCCAG ATTATTGCTA 
TTCTTAACAC ACGCAAAAAA ATTTCGAAGT CGGTTGTCTG AACGACATCA GCTCAGAAGC 
TTGGTAGCTT TGTTAGAACC AACTTTGGAA CCTCAACTCT TATGTCTTTT ACTTCAAGTT 
CTAGCTCTTA TAGCGTTAGA TCCATCTACC CACACAACTT TGATAGACAT TCAAATTGAC 
GACGTCTTGA TTCAAATGCT ACTCCCAGCA GATGATTGGT ACTACACTAA TCACAGCACT 
AAATATGGAA CTTTTGTAAA GTACCATGCT GCTAGAGTTT TAGTGTACGT TGGAATGGGT 
GATCGAGTAG GAAGTCGAGT CAATCTGTTC AACATGATGA CGTTTGAGCC ATCTGCAAAA 
AGCGGGAGTC AACAAAACGA GGACGACTAC ATTTGTGAGA CATGTGCCAC ACCGCGCAGC 
CGAAGTACAT TTTCAAGATC AGCAGTTTCA GTGGAAGGTG TTCTGTTAAA AGTGTTGGCA 
GAAGTTGCTG AGCTTGTACG TCAGAGTCAA CACCTATCAG CAAGTGAACC AATAACTGAA 
GAATCGCCCA GTGCAGGATC TCCACCCGCT GTCCTACCAA ATCAGGCTGA ACAGAAAAGT 
ATTAACGAAA AAAATGCAGA GAAACTTACA CAACTTGGGT GCCAAATTTT GATCTCATTG 
GAACAACTTG AAGCTCATTT ATGCAAACTT GGACTGGTAC TTGATTCGGT TCTACTTCTC 
CGTCTACTTC TTCATAAGTT GTCATGGGAT CTTGGATTAG TCACAAAAAA GAGGGTTGCT 
GTGGTGGATC ATGTTTACAA ACCAATCACG GATCCGAGAG CTCATTCTAG CTGCAGTCTT 
GGAAACAACA GGCGAGAAGA TAGCAAACGG GACAAAAATT ACTTGCGTGT TGATCAGGGA 
TCTCGTGCGT CAAAGAGAGT GCATATCAGA CGATCTAGTT CAGTAGAGAT TATCAGGCCA 
AAACGACTCA GTTCCGGAGC AAAAGATATC AAAAATCGAG AACGGAGGAA AAGGCTGGGA 
ACAGATACAA GTAGCGGGTC GAATCGTTCC AAGAAAACCA ACTCCTCGTC TTCATCTGTT 
CAGAAACACT TGCCAAAATA CATTCAATCA TTATTCCGAA GTCGGATGGG AACTGATCCA 
TGCAAGAGGA CATCGAGAAG AGACTCTAAT GAGAGTAATA CAAGTGGAAG TGATGCTGTT 
CTTGAGTTCA CAAGAAAACT TCAAAATTAT CCGTTGACGA GACGCGAAGC GCAAAGATTG 
ACGTATCGAA GCAATGGGCA ACAAGATTCA AATGACTCGA AGATGGGACC ACGAACTTTA 
GGATACGTTT ACCTCCCAGA ACTAGAAATT CAAGGTGCAA GTCCGCCAAG ATCACCGGGA 
CCTCTAACTG GAAACTCGTT AGAAGAAGGA ACCACAATGC TTTTGGACAC AAGACGGCCG 
AGCAGCCCAC AAGCAATCCC CGGCCTTCCT TTAATTGAAA TTCGTCGGCC CTCAGCTCTC 
TCTCAATTTG AATTTGGATA CTTTGTAAAC TCACCAGAAT TATCAGGAAG TGAAGTATCT 
GATTGTGCCC CATTACTTCT CAGTGGTCAA ATTGGTGGAA GCCGAAAAAG CTCCGACGAA 
ACATCATTGG GAGGATGGTC CAGCCGTGCT TCATCGGTAA TGAGTCAAAG GTCGTCCAGA 
TCCAGTGTTG GCTTGAGGTT ATCAACATTT TCTGGAGGGA CGAGTATTGC TTCGGATAAC 
TCTGGGCCAT TTTTGTTCAG TTTTGTTTTG AGAAAACGTG CATCTACTAT AGGAACTAGA 
ATACCAATAC CTAGGAGAGC TTTGTCAAGA TCTTCAGGAG ACAGTCTGAG AGTACCTGAT 
AGAGAAAGTC CACTTCATTT AGCAAGTATG ACAGAGATGA ATCCGGATTT TCAATGTGTT 
CGACAGCTCA TCTTGAACCT TTTGAATGTT TACACGAAAA GAAATGACAA TGTGGTTTCT 
ACAATGAAGG AATGTGCGGA TGTTCTTCGT CAAATCCTAA ACTCTCCTCA ACATCCAACT 
GTCAAGAATT GGTGTGCCGA AATAATTCAT GTAGTCAGTA CTCATGTAGA AGAAGAGGAA 
CCAACGACGG CAGAAAATAA TGAACAAATT AATGACGAGT ATCTGGAATT TCAAGACCAA 
GTGATATCAG GAAGTTTACC ATGCCCGAAG GAAGAGGCTG CATACCTTGC AAGTATTCAG 
TTATCAGTTG AAGAGCAGTG GCCGAGTAAT AAAAGGACCC AGACAATTAG AAGACATTTA 
TTGAAAGGAC AGTTTGGTCG AATCCGGGAT CTCGCCCAAA AGATCATGGT GACTCCATGG 
GAAGTTGATC AAAACTTGTA CTGCACACCA CCCAGATTTC CCAATGAATC GGCAAATGCT 
AGTAGAGCAC AATCAGTGGT TGAGGAAATT CAGCACAGAT CAAGAACTCC AACTCTTCTG 
CGATGTATCA CAAACACAGA CGGCTTAATG TCAGAAGAGA TGCAAGCTCA GTGCCTTCCC 
GTCGATCTAC GTGGTGATCG TAGAACTATC AAGCTGGTCA AGGAGAGGAA GCGAAAATTG 
TTCCATAGTC AAGTCTATGA AAGTGAAATT GGCATGAAGA AATTGTACAT TCAGACCGCC 
AAAAAACTAG CCGCATTTGG TTGTAAGGTT TTTCAAGTTA AAGAGTTGCT TCACGGACGA 
ACACTCAGAA AAACTCTTCG ACTGCTTTGC CTTTCTTCTG CTCAATTGTG TCTTCTCGAC 
GGATCAACAA AACTTGTGCT TAAACGGCAG CACGCATCGA CGTTACAACA GTGGAGAGTA 
GGTGGTGGAG TTAGTAAGCA TCAACTATTG CTCGAATTCC GTGGAACCAA GTGGCAACTT 
ATTGCACCGT CGTATAATGC ACTGAAATCG ATATCGATGA CATTATGGGA GATTATGCAA 
AATTCTGCTA GCAATTCAAT CCAGCGGAGT CTCAACCAGT CAATGCATCG TTCGCACACC 
GAGGCGACTG TCTCAACATC ACGTAGTGAC TCAACCTCTT CAACGCAATC AGCCACAGCT 
GGATTCATTC TCAATGAAGA ACCAATCACA CTGTTCCGAC TTGAACTCGA ACGACTTCAA 
TATATTCTTC ATTTCCCTGA AGAAGTTGCA TTCCAACTAT CTTCTACTGA GTATCAGCTT 
TTCTACTCAA TACAACCCAT GGATTATGTT CGTTACGTCA GTTGCGATCT CACATCTGTT 
CCAGTGTCAG AAAATCCAAG TCCAGTGCGA AATCTTGTGA AAAGGCTATC AGAGGTATCA 
TCCTGGATAA CACATGTTAT TGTCAGTCAG CCAACACATG ACGATCGAAA GGTTGCTCTT 
ACTGCAATTT TGAGAATTGT GGAAACTTGC TGGAATATTG GAAACTTTAA TGCAGCTGTT 
GAGGTTCTTA TGGGTTTAAA GAGTGAAAAA CTCAGACCAT TCTGGCTTTC TTTGCGACAA 
GAGGAAAAGT CTCAGTTTGA TTCGCTTTGC GAAACCTTGC TTCCTGCAAA CCAAGCTCTC 
CCATCTCAAG CTTACATCAA CGCGGTCCAA CGAGCACTGA GAATGCCACA ATCTCGTGTG 
ATTCCATTTT TTGGAATATT TCTTCGTGAT CTCTACGCAA TTGTGAATGA CTTGCCGAAT 
ATCGTTGTGA TTGGACAAGA AGGTGAAACC CAAAAGCTGG AATTCATGAG CGATCCCAAT 
GGCGAAGATC ATTTTTCGTC GAGAATTGGA GTGGGTGGGC TGTTGAATGC TGATAAAATC 
AACTTGGTGG CAATTGTTTT GGACAACTTG GAATTATTCC ATCGTCACAG CAGGACAATG 
ATCAAATTGT TAGAAGAGCA AGCAGTCCCA CCAATTCAAA TTCCTCAGAA CGAGCGGGAG 
CAAAAAGAAA AGGAAGCAAA AACATATGAA CCAGTACAAG TGGTTCGCGG CTCTTCTCAT 
GGAGTGGCTC TCATCCCTCT TGATACCCTA ACATTCGATC TTGATGTTAT CCAACGACTC 
CAGCACGGAA CAACAGTGAT CCATTACGAA CCTGATTCGG GAAGAAGCAA TTTGTGTCTG 
CTGCGGTTAG ACCCTAGTTG CGGTCAAATA AACTGGCATA AGATTTCATA TTCAGTTAAC 
AAAGATCCCA AAGAAAAGGA TGTGCTCGCC AAAGTATCCG TTTCAAATCT GCAGCCGCTA 
GACTCTGGCC GAGGGGCTCC GTCACCAATG CCATCTGGAA GAACACCAGG AACTGGAGGA 
GTTGGAGTAG AGGAGGGAGA ACTCAAATTA AGTGTTGTGA AAGGAGTCGA ACTAGTTGAT 
AGTTATGATA TTGATATTGA GGCAATTTAC CGTCGTCATT CTATGGAAGA GATGTCAGTT 
CCTGTTAGCT GTTGGAAAGT TAGTCATGGT CAGTTGTTAT CGGATAATGA GTTCATCTAC 
TTTTTGGCCC CTCAACAAAT TGCTCAATTT TGGACCAACG GGTTGCAATC TGTAGTTAAA 
AGTCTCCAAG GACAGCAAAG GTACCCTGAT CGGCGAATGT TATGGATCAA AAATGTCTAC 
TTATCACTTT ACGAGATAAC GGGCGAATCA AACTGTGGAC CACGGCCATT TGAAGCACTT 
CAGGCATTTG GGCTGAGTCA AACAAATACT AACGCAACAC GACCCAATGA CTCGTCGCTG 
TCCAGTGAGC CAGGAGGAGC TAAAAGCAGA CTGAAGAACC TTAAAAATGC TATGCAAAAG 
AAGCTTCGTG GTGCATCGCG CGAAGGAAGC AGATCTCAGT CACCTCAACC TCATTCTCCT 
CTTGTCCGAC CACCATCAAT AAAATCGCAA ATATCGTCAC AATCTGGTCC GCCGGGTCCG 
AACTCTCCAG GCTATTTGCT GAAACCACGT GGAGAACCTG CCAATAGTGA TGCAGGAGAC 
ATTGATAGCA TTTATACACC TCGATCAAGA ACTCCAACAA GCAGCTCCTA TGGAGGGCGA 
TCCGTTGGAG GGAGATCATG TAAATCGTGG AGATCAAGAG GAGGAGAGAC ACCAAATTCG 
GGTTCCATAA GCAGCTCGGG TCAGATGTCA ATTCAAGTGT CTGGTCTGAG TGGACCATCG 
GGAAAAGAGT TTCAGGAAAA ACCATTAACA CTTGTAGAGT TCGCTGAACT TTTTCGATTG 
TTCAATACAA GAATGAGAAA AGATTTGAGA GATGTGTTCA ATGATGTCCT GTCTACGGCA 
ACTACTCCTC AGCATTGTCC TAAAAGAGAA CGTGATCGCC ACAGTCCGAG AATGCAGTCA 
AGGCTGGCGA GTGTCTCCAA CTCGTACAAT GCAGATTTTC TGTCTAACGA TTTCTTGACT 
CGGAATACGG CAGTAACGTC CCATCATATC AGTGAAAAGC AAAACAAAAT CTACAATGCA 
TTGGCACTTG CGAGTGTTAA TAGCATGGGC GGACTCATGG ACACTTCCCG GTCGTCGATG 
CTGACGCCGC AAATGCTGCG CGCTTTTGTC AACACTCATC AGATGGAGCA GATTGACGAG 
CAAACGGCGA TTAAGCTTAT TCAGGATCAT GAACCTGATG GTATCTGTCG ACAAAAGAAT 
CAAATGTCAT TCGAAGGATT CACTAGATTC CTTTGCGATC CTGTCAACTT TGCATTCGTA 
CCGGAAACAA TTGAACCAGA TGAAGAGGAC CTACGGTACC CGTTGTCTCA TTATTACATC 
AATTCAAGTC ACAACACCTA TTTGACGGGA CATCAACTCA AGGGACCCAG TAGCTCGGAG 
ATGTACAGAC AGGTTCTCCT CACCGGGTGT CGGTGTGTCG AGCTTGATTG TTGGGATGGA 
GATGACGGAT TGCCATTGAT TTATCATGGA CACACATTGG TTAGCAAGAT TGGGTTTAGA 
CAGGTGGTCG AAATTATCAA AAAATCTGCA TTCATAACAT CGGATCTTCC TGTGATATTA 
TCAATTGAGA ATCATTGTTC CCTACAACAA CAGGCGAAAA TGGCACAGAT GTTCAAAACA 
GTTCTTGGAG ATTTACTAGT ATCAAACTTC CTTTTTGAAG CTGATTTCTC GGACTCTCCA 
CGTCTTCCAT GCCCTCTCCA AATGAAAAAT AAGATCTTGA TAAAAAATAA GAAAATGATA 
GTCGACCCAC CAACTCCATT GCCAATGATT GAACGAGGAG CTGTGCAGCG TGGTGAAACT 
CAATTAAATC TTCACAGAAA ACAGAGTAAA AACTCCTACG AAAGCAGTAC GGTAGATGAA 
GTGGAAGATG ACGACCTAGA TGAGTTTCTT GACGATGAAG AAAATGAGGA GGATGACCAA 
GAAGAGGTTC AAGTCAGAAG TGAAAAAGAA GACAGTCCGA AGACATCAAA ACGTGCAGAG 
AAATCGGCTA GAAACATCAA ACAGCAAGAT TCGTTATGTA GCGATCATTC GGTAGAGCAG 
GCAAAACCTT CCACAAGCAA GACCACATCC AAAACCAACG ATAGAAAGAC TGAAGATGAA 
GTACTTTACG CTCAACTTGC TCAAAATGCC ATCCGAAACC AACAACCAAG AAAGAACAAT 
ACTGGAGTCC AAATAGCGCC AGAGTTGTCA GATATTGTCA TTTACATGCA AGCGACAAAA 
TTCAAAGGAT TTCCCCCAGT CGATGGCATT CAATCACCAA GGATAATGGA GGAAGGGCCA 
GCTTCTGCAA GTCTTTCATT TTCATCACGG GCTCGTACGC CATCAAATCT GCTGAATACA 
CCAGCCCCTC CACGACGACA GCGAAGTAGC ACTCAGTTGA GTCAAGAACT GGCTGCAGAA 
TTTTTGGGAT CAGTTAGGGC AAATGCTACA GCTACATGCT ACCAGGTTAC ATCTCTTAAT 
GAAAACGCTG CAAAGAAACT GATGAAAAGA CATCCAGCAA AATGCGTATC TTATACTCGG 
GACCATCTGA TTCGTACGTA TCCAAGTGCC AAGCACTATG ACTCGTCCAA CTTTAACCCC 
ATCAACTGCT GGGCTCATGG AATGCAAATG GTTGCTTTAA ATTTTCAAAC TCCTGATGTT 
ATAATGGCAG TTAACCAAGC GATGTTCGAG CAATCTGGAA ACTGTGGATA TCAACTGAAA 
CCACGTTGTC TTTGGGATGA ATCTCATCTT TTGTACAACA AATTTTTGCC TCTCTCCAAA 
GACATTGCCG GCCATTCTGC TTTGTTACTA AACTTAACTA TTATATCCGG ACAGCACGTG 
TATCCAAACA CCCATTACGC CAGTTTATAT GTCGAAATTG AAGTCATCGG TATTCACAAC 
GACTGTGTTC GTGAGAAATC AAAAGTGGTC CAGAGAAATT CTGTGAATCC AATCTGGAAC 
CACACCACTC AACTTCGAAT AGCTTGTGTT GACTTAGCAT TCCTTCGCAT AGCAGTATGT 
GATAGTGGGC AAAATGGAAG AGTCGTTGCT CACCGAGTTG TTCCTGTCAA ATGCATTCGT 
CCGGGTTTCC GACATCTCCC ACTCAGAACT CCAACTAATC TTCCGATCGA CAACGCTATG 
ATATTTTTAA GAACAAGATT CGAGCAAGAA GAACACATCT ACCTCCACGA CGATGATTCA 
AACACCTATT GTAATCTAGA ACATACGCTG GCATACCGCA CAGACTTAAC ACCCAATTTA 
TCTCCAACGC CAATTTTGAA GAAACAGATC TTTGTTCTTC GAATTACAGG CGCGTTTGCT 
GATGAAACAG CAATTACAGT GCACAGCGAG TCAGGAAGTA CAGTGAAGAC GGTGATGCAA 
CAGGCGCTGC TTAACGCTGG AAAGAATGCG GATCAGGTTG AAGAGTATGT ACTCATCGAA 
GAATCACTGC CGGCACCTTC TGGGGAAGAT CCAATTGAGC AGAGAGTACT TCCTCTGAAC 
GAGCCTATCA TGGACGCCGT GGCCTGCTGG AACGGTTCAA TGCGTCGTTT TGTGTTACGA 
AAAAAAGGCA GTGACCCATC ATCTAGAGCT TGGATCACTT CAATTATAAA ATCCGGAACA 
AGTGGAAGCT CCACGTCAGT CTCACCAAGT CCGTTGACCA AAGATGGACA CGTAAAGAGT 
GCATCAAGCA ATCAACTTCA TGGACGATCA TTGGACACTG ACGCTTTTGG AGAGCACCTT 
GAAGTGACAG AAGGGTTGAA CCCTCGTGCA AGATCAATGG GCGACACATT TCTGGTATGT 
GTTCACAATG TTAGTGAAGA TCAGCCTTAT GCAATCCTAC GTGCTGGAAT CCACAGCACT 
GCCGCCGACA TTATTCGTCA GGTGTTTGTT AAAGCTCGCC GTTCAAATGT AGACGATTCC 
GAGTTTGTTC TTGTTGAAGA AACCTGCGAT GATCCAAAAC TCAATCAAGG CCAAATGACT 
CCAAAGTATC CAAACAACCG CACCACAAGC CGAGTGTTAG GACAAAACGA AAATGTTTGG 
AAAGCACAAT CGAGGTGGAA GTCAATGGGC AGATTTGTAC TTGAAAACCG AAAGGACACA 
GTGCACGCAA CCCTCGAAAA AGTGAGATCA TTTATATCAA AACTGGAAGC TGCAAAAGCA 
AGCATGGATC CCAGACACGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360637.1
CDS	1..10464
Translation	MSLRQASHKFPSVDSMPTSRDSSMETTDRYPFGYTTELARTEPSASTAKRLRQRLQRQQSGQSGLSGRRYTGESSECSSLVGTPTDTGRSFTFGAAQRVMRERDDRKPSVTQNLSEIMLEAIHYEDVNLVERLLTAHSSKQPMSTSPSIGSTNTLTDLAQRRHGAGAHRRSNASSTVSTHSGGRSTCAMMNTLHMAVAHKQRDIVELLLKNGYDPNTPASCHCKGNCTATGNIPLTSIIPRTHSMTPELCSTCSQLRVVSIVDQTPLGVAVRSQSSELIALLIAYGGDVNLGDEDGNTPLMLAVRESPLSWPCLHTLIFFGAQIEQKNMRGICPLDLAPELKKLQQTCVEELFKNACSGDCEPPSRSTPGPSAKNWNRLQVDNDPVIDCVSWEQAWELLKKMACNPECLDNIVNSLSKFATQMENTPSSVDRDQFDAHMGGLLHKMIHTAIEEYESSTPTYKKQKKLHLTWLLSQIATFCFTFLQKSGTSRQFSALNTLNKIIDAGLVHDLFAFNDVVFHSSRILNRCHAFDSDEPPTPSGESFNSNADQNSTVDHVFIYGAGYKDQSLLPSPTVAPTRHKSDLMNSFSEMDPSQVIVALHNAITMQNREVGSRSVCSPAHRWRQCCAHCTQILVARLLLFLTHAKKFRSRLSERHQLRSLVALLEPTLEPQLLCLLLQVLALIALDPSTHTTLIDIQIDDVLIQMLLPADDWYYTNHSTKYGTFVKYHAARVLVYVGMGDRVGSRVNLFNMMTFEPSAKSGSQQNEDDYICETCATPRSRSTFSRSAVSVEGVLLKVLAEVAELVRQSQHLSASEPITEESPSAGSPPAVLPNQAEQKSINEKNAEKLTQLGCQILISLEQLEAHLCKLGLVLDSVLLLRLLLHKLSWDLGLVTKKRVAVVDHVYKPITDPRAHSSCSLGNNRREDSKRDKNYLRVDQGSRASKRVHIRRSSSVEIIRPKRLSSGAKDIKNRERRKRLGTDTSSGSNRSKKTNSSSSSVQKHLPKYIQSLFRSRMGTDPCKRTSRRDSNESNTSGSDAVLEFTRKLQNYPLTRREAQRLTYRSNGQQDSNDSKMGPRTLGYVYLPELEIQGASPPRSPGPLTGNSLEEGTTMLLDTRRPSSPQAIPGLPLIEIRRPSALSQFEFGYFVNSPELSGSEVSDCAPLLLSGQIGGSRKSSDETSLGGWSSRASSVMSQRSSRSSVGLRLSTFSGGTSIASDNSGPFLFSFVLRKRASTIGTRIPIPRRALSRSSGDSLRVPDRESPLHLASMTEMNPDFQCVRQLILNLLNVYTKRNDNVVSTMKECADVLRQILNSPQHPTVKNWCAEIIHVVSTHVEEEEPTTAENNEQINDEYLEFQDQVISGSLPCPKEEAAYLASIQLSVEEQWPSNKRTQTIRRHLLKGQFGRIRDLAQKIMVTPWEVDQNLYCTPPRFPNESANASRAQSVVEEIQHRSRTPTLLRCITNTDGLMSEEMQAQCLPVDLRGDRRTIKLVKERKRKLFHSQVYESEIGMKKLYIQTAKKLAAFGCKVFQVKELLHGRTLRKTLRLLCLSSAQLCLLDGSTKLVLKRQHASTLQQWRVGGGVSKHQLLLEFRGTKWQLIAPSYNALKSISMTLWEIMQNSASNSIQRSLNQSMHRSHTEATVSTSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKVRSFISKLEAAKASMDPRHE

Target ORF information:

RefSeq Version	NM_001373489.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373489.1

ORF Insert Sequence:

ATGTCCTTAC GCCAAGCATC ACACAAATTT CCATCGGTGG ACTCAATGCC AACATCACGG 
GATTCATCGA TGGAAACAAC CGACCGGTAC CCGTTTGGGT ATACGACCGA ATTGGCACGT 
ACCGAACCAT CTGCATCAAC TGCCAAACGT CTTCGACAAA GGTTACAGCG ACAGCAAAGT 
GGTCAATCAG GATTATCCGG TCGACGGTAT ACTGGAGAAA GCAGCGAGTG CTCATCACTT 
GTCGGAACAC CCACAGACAC TGGCAGATCA TTTACATTTG GAGCAGCACA AAGAGTCATG 
CGGGAACGGG ATGACCGCAA ACCATCAGTT ACCCAGAACT TGTCAGAAAT AATGCTCGAG 
GCAATACATT ATGAAGATGT GAACTTGGTG GAAAGGTTGC TCACTGCGCA TAGTTCAAAA 
CAACCAATGT CAACATCTCC GTCAATTGGT AGCACAAACA CATTGACGGA TCTTGCTCAA 
CGTAGACACG GTGCGGGGGC ACACCGACGT TCCAATGCCA GTAGTACGGT GTCCACACAT 
TCCGGAGGAA GATCCACGTG TGCTATGATG AACACGTTGC ACATGGCTGT GGCTCATAAA 
CAACGAGATA TAGTGGAACT TTTGCTTAAA AACGGCTACG ATCCCAATAC TCCCGCATCG 
TGTCACTGTA AGGGAAATTG CACGGCGACT GGGAATATTC CACTGACTTC GATCATTCCG 
AGAACTCATT CAATGACGCC AGAATTGTGT TCGACGTGCT CTCAACTGAG GGTTGTGTCA 
ATCGTGGATC AAACACCGCT TGGTGTGGCA GTCAGATCAC AGTCCAGCGA ATTGATAGCA 
TTGTTGATTG CCTACGGAGG AGATGTTAAT TTAGGTGATG AAGATGGCAA CACGCCGTTG 
ATGCTAGCCG TACGAGAATC ACCTCTGTCT TGGCCTTGCC TGCACACGCT CATTTTCTTT 
GGAGCTCAAA TTGAACAAAA GAACATGCGC GGAATATGCC CTCTTGATCT TGCTCCAGAG 
CTGAAGAAAC TTCAACAAAC TTGCGTTGAA GAGCTTTTCA AAAATGCGTG CTCGGGTGAT 
TGTGAACCAC CATCTCGATC TACACCGGGA CCATCAGCCA AAAACTGGAA TAGACTACAA 
GTTGACAATG ATCCTGTAAT TGACTGCGTC AGTTGGGAAC AAGCATGGGA ATTGCTGAAG 
AAGATGGCAT GTAATCCAGA ATGTCTAGAT AACATCGTGA ATTCATTGTC AAAATTTGCA 
ACCCAAATGG AGAACACCCC GTCAAGCGTT GATCGTGATC AATTCGATGC ACACATGGGT 
GGCCTACTAC ATAAAATGAT TCATACAGCG ATTGAGGAGT ACGAGTCTTC GACACCAACC 
TACAAAAAGC AGAAGAAACT CCATCTCACC TGGCTGCTCA GCCAAATTGC CACGTTTTGT 
TTTACATTTC TGCAGAAAAG TGGAACGAGC CGACAATTCT CAGCACTGAA CACGTTGAAT 
AAAATTATTG ATGCTGGTTT AGTACATGAT TTGTTCGCAT TCAACGACGT TGTCTTTCAT 
TCTTCAAGAA TTTTGAACCG ATGCCATGCA TTTGATTCCG ACGAACCACC AACTCCATCT 
GGCGAATCTT TCAACTCAAA TGCAGACCAA AACTCAACTG TCGATCATGT TTTCATTTAC 
GGAGCTGGCT ATAAAGACCA ATCGTTATTA CCTTCTCCTA CGGTGGCACC AACTCGACAC 
AAGTCCGACT TAATGAATTC TTTCAGCGAA ATGGATCCAA GCCAGGTCAT TGTGGCCTTG 
CACAACGCGA TAACAATGCA AAACCGGGAA GTAGGGTCAA GATCTGTTTG TAGTCCGGCT 
CATCGATGGA GGCAGTGTTG TGCTCATTGT ACACAGATAT TGGTGGCCAG ATTATTGCTA 
TTCTTAACAC ACGCAAAAAA ATTTCGAAGT CGGTTGTCTG AACGACATCA GCTCAGAAGC 
TTGGTAGCTT TGTTAGAACC AACTTTGGAA CCTCAACTCT TATGTCTTTT ACTTCAAGTT 
CTAGCTCTTA TAGCGTTAGA TCCATCTACC CACACAACTT TGATAGACAT TCAAATTGAC 
GACGTCTTGA TTCAAATGCT ACTCCCAGCA GATGATTGGT ACTACACTAA TCACAGCACT 
AAATATGGAA CTTTTGTAAA GTACCATGCT GCTAGAGTTT TAGTGTACGT TGGAATGGGT 
GATCGAGTAG GAAGTCGAGT CAATCTGTTC AACATGATGA CGTTTGAGCC ATCTGCAAAA 
AGCGGGAGTC AACAAAACGA GGACGACTAC ATTTGTGAGA CATGTGCCAC ACCGCGCAGC 
CGAAGTACAT TTTCAAGATC AGCAGTTTCA GTGGAAGGTG TTCTGTTAAA AGTGTTGGCA 
GAAGTTGCTG AGCTTGTACG TCAGAGTCAA CACCTATCAG CAAGTGAACC AATAACTGAA 
GAATCGCCCA GTGCAGGATC TCCACCCGCT GTCCTACCAA ATCAGGCTGA ACAGAAAAGT 
ATTAACGAAA AAAATGCAGA GAAACTTACA CAACTTGGGT GCCAAATTTT GATCTCATTG 
GAACAACTTG AAGCTCATTT ATGCAAACTT GGACTGGTAC TTGATTCGGT TCTACTTCTC 
CGTCTACTTC TTCATAAGTT GTCATGGGAT CTTGGATTAG TCACAAAAAA GAGGGTTGCT 
GTGGTGGATC ATGTTTACAA ACCAATCACG GATCCGAGAG CTCATTCTAG CTGCAGTCTT 
GGAAACAACA GGCGAGAAGA TAGCAAACGG GACAAAAATT ACTTGCGTGT TGATCAGGGA 
TCTCGTGCGT CAAAGAGAGT GCATATCAGA CGATCTAGTT CAGTAGAGAT TATCAGGCCA 
AAACGACTCA GTTCCGGAGC AAAAGATATC AAAAATCGAG AACGGAGGAA AAGGCTGGGA 
ACAGATACAA GTAGCGGGTC GAATCGTTCC AAGAAAACCA ACTCCTCGTC TTCATCTGTT 
CAGAAACACT TGCCAAAATA CATTCAATCA TTATTCCGAA GTCGGATGGG AACTGATCCA 
TGCAAGAGGA CATCGAGAAG AGACTCTAAT GAGAGTAATA CAAGTGGAAG TGATGCTGTT 
CTTGAGTTCA CAAGAAAACT TCAAAATTAT CCGTTGACGA GACGCGAAGC GCAAAGATTG 
ACGTATCGAA GCAATGGGCA ACAAGATTCA AATGACTCGA AGATGGGACC ACGAACTTTA 
GGATACGTTT ACCTCCCAGA ACTAGAAATT CAAGGTGCAA GTCCGCCAAG ATCACCGGGA 
CCTCTAACTG GAAACTCGTT AGAAGAAGGA ACCACAATGC TTTTGGACAC AAGACGGCCG 
AGCAGCCCAC AAGCAATCCC CGGCCTTCCT TTAATTGAAA TTCGTCGGCC CTCAGCTCTC 
TCTCAATTTG AATTTGGATA CTTTGTAAAC TCACCAGAAT TATCAGGAAG TGAAGTATCT 
GATTGTGCCC CATTACTTCT CAGTGGTCAA ATTGGTGGAA GCCGAAAAAG CTCCGACGAA 
ACATCATTGG GAGGATGGTC CAGCCGTGCT TCATCGGTAA TGAGTCAAAG GTCGTCCAGA 
TCCAGTGTTG GCTTGAGGTT ATCAACATTT TCTGGAGGGA CGAGTATTGC TTCGGATAAC 
TCTGGGCCAT TTTTGTTCAG TTTTGTTTTG AGAAAACGTG CATCTACTAT AGGAACTAGA 
ATACCAATAC CTAGGAGAGC TTTGTCAAGA TCTTCAGGAG ACAGTCTGAG AGTACCTGAT 
AGAGAAAGTC CACTTCATTT AGCAAGTATG ACAGAGATGA ATCCGGATTT TCAATGTGTT 
CGACAGCTCA TCTTGAACCT TTTGAATGTT TACACGAAAA GAAATGACAA TGTGGTTTCT 
ACAATGAAGG AATGTGCGGA TGTTCTTCGT CAAATCCTAA ACTCTCCTCA ACATCCAACT 
GTCAAGAATT GGTGTGCCGA AATAATTCAT GTAGTCAGTA CTCATGTAGA AGAAGAGGAA 
CCAACGACGG CAGAAAATAA TGAACAAATT AATGACGAGT ATCTGGAATT TCAAGACCAA 
GTGATATCAG GAAGTTTACC ATGCCCGAAG GAAGAGGCTG CATACCTTGC AAGTATTCAG 
TTATCAGTTG AAGAGCAGTG GCCGAGTAAT AAAAGGACCC AGACAATTAG AAGACATTTA 
TTGAAAGGAC AGTTTGGTCG AATCCGGGAT CTCGCCCAAA AGATCATGGT GACTCCATGG 
GAAGTTGATC AAAACTTGTA CTGCACACCA CCCAGATTTC CCAATGAATC GGCAAATGCT 
AGTAGAGCAC AATCAGTGGT TGAGGAAATT CAGCACAGAT CAAGAACTCC AACTCTTCTG 
CGATGTATCA CAAACACAGA CGGCTTAATG TCAGAAGAGA TGCAAGCTCA GTGCCTTCCC 
GTCGATCTAC GTGGTGATCG TAGAACTATC AAGCTGGTCA AGGAGAGGAA GCGAAAATTG 
TTCCATAGTC AAGTCTATGA AAGTGAAATT GGCATGAAGA AATTGTACAT TCAGACCGCC 
AAAAAACTAG CCGCATTTGG TTGTAAGGTT TTTCAAGTTA AAGAGTTGCT TCACGGACGA 
ACACTCAGAA AAACTCTTCG ACTGCTTTGC CTTTCTTCTG CTCAATTGTG TCTTCTCGAC 
GGATCAACAA AACTTGTGCT TAAACGGCAG CACGCATCGA CGTTACAACA GTGGAGAGTA 
GGTGGTGGAG TTAGTAAGCA TCAACTATTG CTCGAATTCC GTGGAACCAA GTGGCAACTT 
ATTGCACCGT CGTATAATGC ACTGAAATCG ATATCGATGA CATTATGGGA GATTATGCAA 
AATTCTGCTA GCAATTCAAT CCAGCGGAGT CTCAACCAGT CAATGCATCG TTCGCACACC 
GAGGCGACTG TCTCAACATC ACGTAGTGAC TCAACCTCTT CAACGCAATC AGCCACAGCT 
GGATTCATTC TCAATGAAGA ACCAATCACA CTGTTCCGAC TTGAACTCGA ACGACTTCAA 
TATATTCTTC ATTTCCCTGA AGAAGTTGCA TTCCAACTAT CTTCTACTGA GTATCAGCTT 
TTCTACTCAA TACAACCCAT GGATTATGTT CGTTACGTCA GTTGCGATCT CACATCTGTT 
CCAGTGTCAG AAAATCCAAG TCCAGTGCGA AATCTTGTGA AAAGGCTATC AGAGGTATCA 
TCCTGGATAA CACATGTTAT TGTCAGTCAG CCAACACATG ACGATCGAAA GGTTGCTCTT 
ACTGCAATTT TGAGAATTGT GGAAACTTGC TGGAATATTG GAAACTTTAA TGCAGCTGTT 
GAGGTTCTTA TGGGTTTAAA GAGTGAAAAA CTCAGACCAT TCTGGCTTTC TTTGCGACAA 
GAGGAAAAGT CTCAGTTTGA TTCGCTTTGC GAAACCTTGC TTCCTGCAAA CCAAGCTCTC 
CCATCTCAAG CTTACATCAA CGCGGTCCAA CGAGCACTGA GAATGCCACA ATCTCGTGTG 
ATTCCATTTT TTGGAATATT TCTTCGTGAT CTCTACGCAA TTGTGAATGA CTTGCCGAAT 
ATCGTTGTGA TTGGACAAGA AGGTGAAACC CAAAAGCTGG AATTCATGAG CGATCCCAAT 
GGCGAAGATC ATTTTTCGTC GAGAATTGGA GTGGGTGGGC TGTTGAATGC TGATAAAATC 
AACTTGGTGG CAATTGTTTT GGACAACTTG GAATTATTCC ATCGTCACAG CAGGACAATG 
ATCAAATTGT TAGAAGAGCA AGCAGTCCCA CCAATTCAAA TTCCTCAGAA CGAGCGGGAG 
CAAAAAGAAA AGGAAGCAAA AACATATGAA CCAGTACAAG TGGTTCGCGG CTCTTCTCAT 
GGAGTGGCTC TCATCCCTCT TGATACCCTA ACATTCGATC TTGATGTTAT CCAACGACTC 
CAGCACGGAA CAACAGTGAT CCATTACGAA CCTGATTCGG GAAGAAGCAA TTTGTGTCTG 
CTGCGGTTAG ACCCTAGTTG CGGTCAAATA AACTGGCATA AGATTTCATA TTCAGTTAAC 
AAAGATCCCA AAGAAAAGGA TGTGCTCGCC AAAGTATCCG TTTCAAATCT GCAGCCGCTA 
GACTCTGGCC GAGGGGCTCC GTCACCAATG CCATCTGGAA GAACACCAGG AACTGGAGGA 
GTTGGAGTAG AGGAGGGAGA ACTCAAATTA AGTGTTGTGA AAGGAGTCGA ACTAGTTGAT 
AGTTATGATA TTGATATTGA GGCAATTTAC CGTCGTCATT CTATGGAAGA GATGTCAGTT 
CCTGTTAGCT GTTGGAAAGT TAGTCATGGT CAGTTGTTAT CGGATAATGA GTTCATCTAC 
TTTTTGGCCC CTCAACAAAT TGCTCAATTT TGGACCAACG GGTTGCAATC TGTAGTTAAA 
AGTCTCCAAG GACAGCAAAG GTACCCTGAT CGGCGAATGT TATGGATCAA AAATGTCTAC 
TTATCACTTT ACGAGATAAC GGGCGAATCA AACTGTGGAC CACGGCCATT TGAAGCACTT 
CAGGCATTTG GGCTGAGTCA AACAAATACT AACGCAACAC GACCCAATGA CTCGTCGCTG 
TCCAGTGAGC CAGGAGGAGC TAAAAGCAGA CTGAAGAACC TTAAAAATGC TATGCAAAAG 
AAGCTTCGTG GTGCATCGCG CGAAGGAAGC AGATCTCAGT CACCTCAACC TCATTCTCCT 
CTTGTCCGAC CACCATCAAT AAAATCGCAA ATATCGTCAC AATCTGGTCC GCCGGGTCCG 
AACTCTCCAG GCTATTTGCT GAAACCACGT GGAGAACCTG CCAATAGTGA TGCAGGAGAC 
ATTGATAGCA TTTATACACC TCGATCAAGA ACTCCAACAA GCAGCTCCTA TGGAGGGCGA 
TCCGTTGGAG GGAGATCATG TAAATCGTGG AGATCAAGAG GAGGAGAGAC ACCAAATTCG 
GGTTCCATAA GCAGCTCGGG TCAGATGTCA ATTCAAGTGT CTGGTCTGAG TGGACCATCG 
GGAAAAGAGT TTCAGGAAAA ACCATTAACA CTTGTAGAGT TCGCTGAACT TTTTCGATTG 
TTCAATACAA GAATGAGAAA AGATTTGAGA GATGTGTTCA ATGATGTCCT GTCTACGGCA 
ACTACTCCTC AGCATTGTCC TAAAAGAGAA CGTGATCGCC ACAGTCCGAG AATGCAGTCA 
AGGCTGGCGA GTGTCTCCAA CTCGTACAAT GCAGATTTTC TGTCTAACGA TTTCTTGACT 
CGGAATACGG CAGTAACGTC CCATCATATC AGTGAAAAGC AAAACAAAAT CTACAATGCA 
TTGGCACTTG CGAGTGTTAA TAGCATGGGC GGACTCATGG ACACTTCCCG GTCGTCGATG 
CTGACGCCGC AAATGCTGCG CGCTTTTGTC AACACTCATC AGATGGAGCA GATTGACGAG 
CAAACGGCGA TTAAGCTTAT TCAGGATCAT GAACCTGATG GTATCTGTCG ACAAAAGAAT 
CAAATGTCAT TCGAAGGATT CACTAGATTC CTTTGCGATC CTGTCAACTT TGCATTCGTA 
CCGGAAACAA TTGAACCAGA TGAAGAGGAC CTACGGTACC CGTTGTCTCA TTATTACATC 
AATTCAAGTC ACAACACCTA TTTGACGGGA CATCAACTCA AGGGACCCAG TAGCTCGGAG 
ATGTACAGAC AGGTTCTCCT CACCGGGTGT CGGTGTGTCG AGCTTGATTG TTGGGATGGA 
GATGACGGAT TGCCATTGAT TTATCATGGA CACACATTGG TTAGCAAGAT TGGGTTTAGA 
CAGGTGGTCG AAATTATCAA AAAATCTGCA TTCATAACAT CGGATCTTCC TGTGATATTA 
TCAATTGAGA ATCATTGTTC CCTACAACAA CAGGCGAAAA TGGCACAGAT GTTCAAAACA 
GTTCTTGGAG ATTTACTAGT ATCAAACTTC CTTTTTGAAG CTGATTTCTC GGACTCTCCA 
CGTCTTCCAT GCCCTCTCCA AATGAAAAAT AAGATCTTGA TAAAAAATAA GAAAATGATA 
GTCGACCCAC CAACTCCATT GCCAATGATT GAACGAGGAG CTGTGCAGCG TGGTGAAACT 
CAATTAAATC TTCACAGAAA ACAGAGTAAA AACTCCTACG AAAGCAGTAC GGTAGATGAA 
GTGGAAGATG ACGACCTAGA TGAGTTTCTT GACGATGAAG AAAATGAGGA GGATGACCAA 
GAAGAGGTTC AAGTCAGAAG TGAAAAAGAA GACAGTCCGA AGACATCAAA ACGTGCAGAG 
AAATCGGCTA GAAACATCAA ACAGCAAGAT TCGTTATGTA GCGATCATTC GGTAGAGCAG 
GCAAAACCTT CCACAAGCAA GACCACATCC AAAACCAACG ATAGAAAGAC TGAAGATGAA 
GTACTTTACG CTCAACTTGC TCAAAATGCC ATCCGAAACC AACAACCAAG AAAGAACAAT 
ACTGGAGTCC AAATAGCGCC AGAGTTGTCA GATATTGTCA TTTACATGCA AGCGACAAAA 
TTCAAAGGAT TTCCCCCAGT CGATGGCATT CAATCACCAA GGATAATGGA GGAAGGGCCA 
GCTTCTGCAA GTCTTTCATT TTCATCACGG GCTCGTACGC CATCAAATCT GCTGAATACA 
CCAGCCCCTC CACGACGACA GCGAAGTAGC ACTCAGTTGA GTCAAGAACT GGCTGCAGAA 
TTTTTGGGAT CAGTTAGGGC AAATGCTACA GCTACATGCT ACCAGGTTAC ATCTCTTAAT 
GAAAACGCTG CAAAGAAACT GATGAAAAGA CATCCAGCAA AATGCGTATC TTATACTCGG 
GACCATCTGA TTCGTACGTA TCCAAGTGCC AAGCACTATG ACTCGTCCAA CTTTAACCCC 
ATCAACTGCT GGGCTCATGG AATGCAAATG GTTGCTTTAA ATTTTCAAAC TCCTGATGTT 
ATAATGGCAG TTAACCAAGC GATGTTCGAG CAATCTGGAA ACTGTGGATA TCAACTGAAA 
CCACGTTGTC TTTGGGATGA ATCTCATCTT TTGTACAACA AATTTTTGCC TCTCTCCAAA 
GACATTGCCG GCCATTCTGC TTTGTTACTA AACTTAACTA TTATATCCGG ACAGCACGTG 
TATCCAAACA CCCATTACGC CAGTTTATAT GTCGAAATTG AAGTCATCGG TATTCACAAC 
GACTGTGTTC GTGAGAAATC AAAAGTGGTC CAGAGAAATT CTGTGAATCC AATCTGGAAC 
CACACCACTC AACTTCGAAT AGCTTGTGTT GACTTAGCAT TCCTTCGCAT AGCAGTATGT 
GATAGTGGGC AAAATGGAAG AGTCGTTGCT CACCGAGTTG TTCCTGTCAA ATGCATTCGT 
CCGGGTTTCC GACATCTCCC ACTCAGAACT CCAACTAATC TTCCGATCGA CAACGCTATG 
ATATTTTTAA GAACAAGATT CGAGCAAGAA GAACACATCT ACCTCCACGA CGATGATTCA 
AACACCTATT GTAATCTAGA ACATACGCTG GCATACCGCA CAGACTTAAC ACCCAATTTA 
TCTCCAACGC CAATTTTGAA GAAACAGATC TTTGTTCTTC GAATTACAGG CGCGTTTGCT 
GATGAAACAG CAATTACAGT GCACAGCGAG TCAGGAAGTA CAGTGAAGAC GGTGATGCAA 
CAGGCGCTGC TTAACGCTGG AAAGAATGCG GATCAGGTTG AAGAGTATGT ACTCATCGAA 
GAATCACTGC CGGCACCTTC TGGGGAAGAT CCAATTGAGC AGAGAGTACT TCCTCTGAAC 
GAGCCTATCA TGGACGCCGT GGCCTGCTGG AACGGTTCAA TGCGTCGTTT TGTGTTACGA 
AAAAAAGGCA GTGACCCATC ATCTAGAGCT TGGATCACTT CAATTATAAA ATCCGGAACA 
AGTGGAAGCT CCACGTCAGT CTCACCAAGT CCGTTGACCA AAGATGGACA CGTAAAGAGT 
GCATCAAGCA ATCAACTTCA TGGACGATCA TTGGACACTG ACGCTTTTGG AGAGCACCTT 
GAAGTGACAG AAGGGTTGAA CCCTCGTGCA AGATCAATGG GCGACACATT TCTGGTATGT 
GTTCACAATG TTAGTGAAGA TCAGCCTTAT GCAATCCTAC GTGCTGGAAT CCACAGCACT 
GCCGCCGACA TTATTCGTCA GGTGTTTGTT AAAGCTCGCC GTTCAAATGT AGACGATTCC 
GAGTTTGTTC TTGTTGAAGA AACCTGCGAT GATCCAAAAC TCAATCAAGG CCAAATGACT 
CCAAAGTATC CAAACAACCG CACCACAAGC CGAGTGTTAG GACAAAACGA AAATGTTTGG 
AAAGCACAAT CGAGGTGGAA GTCAATGGGC AGATTTGTAC TTGAAAACCG AAAGGACACA 
GTGCACGCAA CCCTCGAAAA AGTGAGATCA TTTATATCAA AACTGGAAGC TGCAAAAGCA 
AGCATGGATC CCAGACACGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_001373495.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360638.1 Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.

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CloneID	OCa851225
Clone ID Related Accession (Same CDS sequence)	NM_001373495.1
Accession Version	NM_001373495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	PhosphoLipase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on the 5' end.

ATGTCCTTAC GCCAAGCATC ACACAAATTT CCATCGGTGG ACTCAATGCC AACATCACGG 
GATTCATCGA TGGAAACAAC CGACCGGTAC CCGTTTGGGT ATACGACCGA ATTGGCACGT 
ACCGAACCAT CTGCATCAAC TGCCAAACGT CTTCGACAAA GGTTACAGCG ACAGCAAAGT 
GGTCAATCAG GATTATCCGG TCGACGGTAT ACTGGAGAAA GCAGCGAGTG CTCATCACTT 
GTCGGAACAC CCACAGACAC TGGCAGATCA TTTACATTTG GAGCAGCACA AAGAGTCATG 
CGGGAACGGG ATGACCGCAA ACCATCAGTT ACCCAGAACT TGTCAGAAAT AATGCTCGAG 
GCAATACATT ATGAAGATGT GAACTTGGTG GAAAGGTTGC TCACTGCGCA TAGTTCAAAA 
CAACCAATGT CAACATCTCC GTCAATTGGT AGCACAAACA CATTGACGGA TCTTGCTCAA 
CGTAGACACG GTGCGGGGGC ACACCGACGT TCCAATGCCA GTAGTACGGT GTCCACACAT 
TCCGGAGGAA GATCCACGTG TGCTATGATG AACACGTTGC ACATGGCTGT GGCTCATAAA 
CAACGAGATA TAGTGGAACT TTTGCTTAAA AACGGCTACG ATCCCAATAC TCCCGCATCG 
TGTCACTGTA AGGGAAATTG CACGGCGACT GGGAATATTC CACTGACTTC GATCATTCCG 
AGAACTCATT CAATGACGCC AGAATTGTGT TCGACGTGCT CTCAACTGAG GGTTGTGTCA 
ATCGTGGATC AAACACCGCT TGGTGTGGCA GTCAGATCAC AGTCCAGCGA ATTGATAGCA 
TTGTTGATTG CCTACGGAGG AGATGTTAAT TTAGGTGATG AAGATGGCAA CACGCCGTTG 
ATGCTAGCCG TACGAGAATC ACCTCTGTCT TGGCCTTGCC TGCACACGCT CATTTTCTTT 
GGAGCTCAAA TTGAACAAAA GAACATGCGC GGAATATGCC CTCTTGATCT TGCTCCAGAG 
CTGAAGAAAC TTCAACAAAC TTGCGTTGAA GAGCTTTTCA AAAATGCGTG CTCGGGTGAT 
TGTGAACCAC CATCTCGATC TACACCGGGA CCATCAGCCA AAAACTGGAA TAGACTACAA 
GTTGACAATG TTTCCCAAGG AGAACGATCA CTGTCAAAAG CTCCATTGAG CCCAAAACCG 
TCTGCTGCAG CAAGTGTCTC AACGTGTAGC ATGCTGGAAA CCTCATCAGC AAAAGAATGT 
GCACGACGAA AATCATTGGT GTCGCTTCAA CTTCACAGAA AAAGCCGAAA CGTGAAAGAA 
TATCCTGTAA TTGACTGCGT CAGTTGGGAA CAAGCATGGG AATTGCTGAA GAAGATGGCA 
TGTAATCCAG AATGTCTAGA TAACATCGTG AATTCATTGT CAAAATTTGC AACCCAAATG 
GAGAACACCC CGTCAAGCGT TGATCGTGAT CAATTCGATG CACACATGGG TGGCCTACTA 
CATAAAATGA TTCATACAGC GATTGAGGAG TACGAGTCTT CGACACCAAC CTACAAAAAG 
CAGAAGAAAC TCCATCTCAC CTGGCTGCTC AGCCAAATTG CCACGTTTTG TTTTACATTT 
CTGCAGAAAA GTGGAACGAG CCGACAATTC TCAGCACTGA ACACGTTGAA TAAAATTATT 
GATGCTGGTT TAGTACATGA TTTGTTCGCA TTCAACGACG TTGTCTTTCA TTCTTCAAGA 
ATTTTGAACC GATGCCATGC ATTTGATTCC GACGAACCAC CAACTCCATC TGGCGAATCT 
TTCAACTCAA ATGCAGACCA AAACTCAACT GTCGATCATG TTTTCATTTA CGGAGCTGGC 
TATAAAGACC AATCGTTATT ACCTTCTCCT ACGGTGGCAC CAACTCGACA CAAGTCCGAC 
TTAATGAATT CTTTCAGCGA AATGGATCCA AGCCAGGTCA TTGTGGCCTT GCACAACGCG 
ATAACAATGC AAAACCGGGA AGTAGGGTCA AGATCTGTTT GTAGTCCGGC TCATCGATGG 
AGGCAGTGTT GTGCTCATTG TACACAGATA TTGGTGGCCA GATTATTGCT ATTCTTAACA 
CACGCAAAAA AATTTCGAAG TCGGTTGTCT GAACGACATC AGCTCAGAAG CTTGGTAGCT 
TTGTTAGAAC CAACTTTGGA ACCTCAACTC TTATGTCTTT TACTTCAAGT TCTAGCTCTT 
ATAGCGTTAG ATCCATCTAC CCACACAACT TTGATAGACA TTCAAATTGA CGACGTCTTG 
ATTCAAATGC TACTCCCAGC AGATGATTGG TACTACACTA ATCACAGCAC TAAATATGGA 
ACTTTTGTAA AGTACCATGC TGCTAGAGTT TTAGTGTACG TTGGAATGGG TGATCGAGTA 
GGAAGTCGAG TCAATCTGTT CAACATGATG ACGTTTGAGC CATCTGCAAA AAGCGGGAGT 
CAACAAAACG AGGACGACTA CATTTGTGAG ACATGTGCCA CACCGCGCAG CCGAAGTACA 
TTTTCAAGAT CAGCAGTTTC AGTGGAAGGT GTTCTGTTAA AAGTGTTGGC AGAAGTTGCT 
GAGCTTGTAC GTCAGAGTCA ACACCTATCA GCAAGTGAAC CAATAACTGA AGAATCGCCC 
AGTGCAGGAT CTCCACCCGC TGTCCTACCA AATCAGGCTG AACAGAAAAG TATTAACGAA 
AAAAATGCAG AGAAACTTAC ACAACTTGGG TGCCAAATTT TGATCTCATT GGAACAACTT 
GAAGCTCATT TATGCAAACT TGGACTGGTA CTTGATTCGG TTCTACTTCT CCGTCTACTT 
CTTCATAAGT TGTCATGGGA TCTTGGATTA GTCACAAAAA AGAGGGTTGC TGTGGTGGAT 
CATGTTTACA AACCAATCAC GGATCCGAGA GCTCATTCTA GCTGCAGTCT TGGAAACAAC 
AGAAACTTTG GTAAATCTAA ATCTTTTGAT AGGCGAGAAG ATAGCAAACG GGACAAAAAT 
TACTTGCGTG TTGATCAGGG ATCTCGTGCG TCAAAGAGAG TGCATATCAG ACGATCTAGT 
TCAGTAGAGA TTATCAGGCC AAAACGACTC AGTTCCGGAG CAAAAGATAT CAAAAATCGA 
GAACGGAGGA AAAGGCTGGG AACAGATACA AGTAGCGGGT CGAATCGTTC CAAGAAAACC 
AACTCCTCGT CTTCATCTGT TCAGAAACAC TTGCCAAAAT ACATTCAATC ATTATTCCGA 
AGTCGGATGG GAACTGATCC ATGCAAGAGG ACATCGAGAA GAGACTCTAA TGAGAGTAAT 
ACAAGTGGAA GTGATGCTGT TCTTGAGTTC ACAAGAAAAC TTCAAAATTA TCCGTTGACG 
AGACGCGAAG CGCAAAGATT GACGTATCGA AGCAATGGGC AACAAGATTC AAATGACTCG 
AAGATGGGAC CACGAACTTT AGGATACGTT TACCTCCCAG AACTAGAAAT TCAAGGTGCA 
AGTCCGCCAA GATCACCGGG ACCTCTAACT GGAAACTCGT TAGAAGAAGG AACCACAATG 
CTTTTGGACA CAAGACGGCC GAGCAGCCCA CAAGCAATCC CCGGCCTTCC TTTAATTGAA 
ATTCGTCGGC CCTCAGCTCT CTCTCAATTT GAATTTGGAT ACTTTGTAAA CTCACCAGAA 
TTATCAGGAA GTGAAGTATC TGATTGTGCC CCATTACTTC TCAGTGGTCA AATTGGTGGA 
AGCCGAAAAA GCTCCGACGA AACATCATTG GGAGGATGGT CCAGCCGTGC TTCATCGGTA 
ATGAGTCAAA GGTCGTCCAG ATCCAGTGTT GGCTTGAGGT TATCAACATT TTCTGGAGGG 
ACGAGTATTG CTTCGGATAA CTCTGGGCCA TTTTTGTTCA GTTTTGTTTT GAGAAAACGT 
GCATCTACTA TAGGAACTAG AATACCAATA CCTAGGAGAG CTTTGTCAAG ATCTTCAGGA 
GACAGTCTGA GAGTACCTGA TAGAGAAAGT CCACTTCATT TAGCAAGTAT GACAGAGATG 
AATCCGGATT TTCAATGTGT TCGACAGCTC ATCTTGAACC TTTTGAATGT TTACACGAAA 
AGAAATGACA ATGTGGTTTC TACAATGAAG GAATGTGCGG ATGTTCTTCG TCAAATCCTA 
AACTCTCCTC AACATCCAAC TGTCAAGAAT TGGTGTGCCG AAATAATTCA TGTAGTCAGT 
ACTCATGTAG AAGAAGAGGA ACCAACGACG GCAGAAAATA ATGAACAAAT TAATGACGAG 
TATCTGGAAT TTCAAGACCA AGTGATATCA GGAAGTTTAC CATGCCCGAA GGAAGAGGCT 
GCATACCTTG CAAGTATTCA GTTATCAGTT GAAGAGCAGT GGCCGAGTAA TAAAAGGACC 
CAGACAATTA GAAGACATTT ATTGAAAGGA CAGTTTGGTC GAATCCGGGA TCTCGCCCAA 
AAGATCATGG TGACTCCATG GGAAGTTGAT CAAAACTTGT ACTGCACACC ACCCAGATTT 
CCCAATGAAT CGGCAAATGC TAGTAGAGCA CAATCAGTGG TTGAGGAAAT TCAGCACAGA 
TCAAGAACTC CAACTCTTCT GCGATGTATC ACAAACACAG ACGGCTTAAT GTCAGAAGAG 
ATGCAAGCTC AGTGCCTTCC CGTCGATCTA CGTGGTGATC GTAGAACTAT CAAGCTGGTC 
AAGGAGAGGA AGCGAAAATT GTTCCATAGT CAAGTCTATG AAAGTGAAAT TGGCATGAAG 
AAATTGTACA TTCAGACCGC CAAAAAACTA GCCGCATTTG GTTGTAAGGT TTTTCAAGTT 
AAAGAGTTGC TTCACGGACG AACACTCAGA AAAACTCTTC GACTGCTTTG CCTTTCTTCT 
GCTCAATTGT GTCTTCTCGA CGGATCAACA AAACTTGTGC TTAAACGGCA GCACGCATCG 
ACGTTACAAC AGTGGAGAGT AGGTGGTGGA GTTAGTAAGC ATCAACTATT GCTCGAATTC 
CGTGGAACCA AGTGGCAACT TATTGCACCG TCGTATAATG CACTGAAATC GATATCGATG 
ACATTATGGG AGATTATGCA AAATTCTGCT AGCAATTCAA TCCAGCGGAG TCTCAACCAG 
TCAATGCATC GTTCGCACAC CGAGGCGACT GTCTCAACAT CACGTAGTGA CTCAACCTCT 
TCAACGCAAT CAGCCACAGC TGGATTCATT CTCAATGAAG AACCAATCAC ACTGTTCCGA 
CTTGAACTCG AACGACTTCA ATATATTCTT CATTTCCCTG AAGAAGTTGC ATTCCAACTA 
TCTTCTACTG AGTATCAGCT TTTCTACTCA ATACAACCCA TGGATTATGT TCGTTACGTC 
AGTTGCGATC TCACATCTGT TCCAGTGTCA GAAAATCCAA GTCCAGTGCG AAATCTTGTG 
AAAAGGCTAT CAGAGGTATC ATCCTGGATA ACACATGTTA TTGTCAGTCA GCCAACACAT 
GACGATCGAA AGGTTGCTCT TACTGCAATT TTGAGAATTG TGGAAACTTG CTGGAATATT 
GGAAACTTTA ATGCAGCTGT TGAGGTTCTT ATGGGTTTAA AGAGTGAAAA ACTCAGACCA 
TTCTGGCTTT CTTTGCGACA AGAGGAAAAG TCTCAGTTTG ATTCGCTTTG CGAAACCTTG 
CTTCCTGCAA ACCAAGCTCT CCCATCTCAA GCTTACATCA ACGCGGTCCA ACGAGCACTG 
AGAATGCCAC AATCTCGTGT GATTCCATTT TTTGGAATAT TTCTTCGTGA TCTCTACGCA 
ATTGTGAATG ACTTGCCGAA TATCGTTGTG ATTGGACAAG AAGGTGAAAC CCAAAAGCTG 
GAATTCATGA GCGATCCCAA TGGCGAAGAT CATTTTTCGT CGAGAATTGG AGTGGGTGGG 
CTGTTGAATG CTGATAAAAT CAACTTGGTG GCAATTGTTT TGGACAACTT GGAATTATTC 
CATCGTCACA GCAGGACAAT GATCAAATTG TTAGAAGAGC AAGCAGTCCC ACCAATTCAA 
ATTCCTCAGA ACGAGCGGGA GCAAAAAGAA AAGGAAGCAA AAACATATGA ACCAGTACAA 
GTGGTTCGCG GCTCTTCTCA TGGAGTGGCT CTCATCCCTC TTGATACCCT AACATTCGAT 
CTTGATGTTA TCCAACGACT CCAGCACGGA ACAACAGTGA TCCATTACGA ACCTGATTCG 
GGAAGAAGCA ATTTGTGTCT GCTGCGGTTA GACCCTAGTT GCGGTCAAAT AAACTGGCAT 
AAGATTTCAT ATTCAGTTAA CAAAGATCCC AAAGAAAAGG ATGTGCTCGC CAAAGTATCC 
GTTTCAAATC TGCAGCCGCT AGACTCTGGC CGAGGGGCTC CGTCACCAAT GCCATCTGGA 
AGAACACCAG GAACTGGAGG AGTTGGAGTA GAGGAGGGAG AACTCAAATT AAGTGTTGTG 
AAAGGAGTCG AACTAGTTGA TAGTTATGAT ATTGATATTG AGGCAATTTA CCGTCGTCAT 
TCTATGGAAG AGATGTCAGT TCCTGTTAGC TGTTGGAAAG TTAGTCATGG TCAGTTGTTA 
TCGGATAATG AGTTCATCTA CTTTTTGGCC CCTCAACAAA TTGCTCAATT TTGGACCAAC 
GGGTTGCAAT CTGTAGTTAA AAGTCTCCAA GGACAGCAAA GGTACCCTGA TCGGCGAATG 
TTATGGATCA AAAATGTCTA CTTATCACTT TACGAGATAA CGGGCGAATC AAACTGTGGA 
CCACGGCCAT TTGAAGCACT TCAGGCATTT GGGCTGAGTC AAACAAATAC TAACGCAACA 
CGACCCAATG ACTCGTCGCT GTCCAGTGAG CCAGGAGGAG CTAAAAGCAG ACTGAAGAAC 
CTTAAAAATG CTATGCAAAA GAAGCTTCGT GGTGCATCGC GCGAAGGAAG CAGATCTCAG 
TCACCTCAAC CTCATTCTCC TCTTGTCCGA CCACCATCAA TAAAATCGCA AATATCGTCA 
CAATCTGGTC CGCCGGGTCC GAACTCTCCA GGCTATTTGC TGAAACCACG TGGAGAACCT 
GCCAATAGTG ATGCAGGAGA CATTGATAGC ATTTATACAC CTCGATCAAG AACTCCAACA 
AGCAGCTCCT ATGGAGGGCG ATCCGTTGGA GGGAGATCAT GTAAATCGTG GAGATCAAGA 
GGAGGAGAGA CACCAAATTC GGGTTCCATA AGCAGCTCGG GTCAGATGTC AATTCAAGTG 
TCTGGTCTGA GTGGACCATC GGGAAAAGAG TTTCAGGAAA AACCATTAAC ACTTGTAGAG 
TTCGCTGAAC TTTTTCGATT GTTCAATACA AGAATGAGAA AAGATTTGAG AGATGTGTTC 
AATGATGTCC TGTCTACGGC AACTACTCCT CAGCATTGTC CTAAAAGAGA ACGTGATCGC 
CACAGTCCGA GAATGCAGTC AAGGCTGGCG AGTGTCTCCA ACTCGTACAA TGCAGATTTT 
CTGTCTAACG ATTTCTTGAC TCGGAATACG GCAGTAACGT CCCATCATAT CAGTGAAAAG 
CAAAACAAAA TCTACAATGC ATTGGCACTT GCGAGTGTTA ATAGCATGGG CGGACTCATG 
GACACTTCCC GGTCGTCGAT GCTGACGCCG CAAATGCTGC GCGCTTTTGT CAACACTCAT 
CAGATGGAGC AGATTGACGA GCAAACGGCG ATTAAGCTTA TTCAGGATCA TGAACCTGAT 
GGTATCTGTC GACAAAAGAA TCAAATGTCA TTCGAAGGAT TCACTAGATT CCTTTGCGAT 
CCTGTCAACT TTGCATTCGT ACCGGAAACA ATTGAACCAG ATGAAGAGGA CCTACGGTAC 
CCGTTGTCTC ATTATTACAT CAATTCAAGT CACAACACCT ATTTGACGGG ACATCAACTC 
AAGGGACCCA GTAGCTCGGA GATGTACAGA CAGGTTCTCC TCACCGGGTG TCGGTGTGTC 
GAGCTTGATT GTTGGGATGG AGATGACGGA TTGCCATTGA TTTATCATGG ACACACATTG 
GTTAGCAAGA TTGGGTTTAG ACAGGTGGTC GAAATTATCA AAAAATCTGC ATTCATAACA 
TCGGATCTTC CTGTGATATT ATCAATTGAG AATCATTGTT CCCTACAACA ACAGGCGAAA 
ATGGCACAGA TGTTCAAAAC AGTTCTTGGA GATTTACTAG TATCAAACTT CCTTTTTGAA 
GCTGATTTCT CGGACTCTCC ACGTCTTCCA TGCCCTCTCC AAATGAAAAA TAAGATCTTG 
ATAAAAAATA AGAAAATGAT AGTCGACCCA CCAACTCCAT TGCCAATGAT TGAACGAGGA 
GCTGTGCAGC GTGGTGAAAC TCAATTAAAT CTTCACAGAA AACAGAGTAA AAACTCCTAC 
GAAAGCAGTA CGGTAGATGA AGTGGAAGAT GACGACCTAG ATGAGTTTCT TGACGATGAA 
GAAAATGAGG AGGATGACCA AGAAGAGGTT CAAGTCAGAA GTGAAAAAGA AGACAGTCCG 
AAGACATCAA AACGTGCAGA GAAATCGGCT AGAAACATCA AACAGCAAGA TTCGTTATGT 
AGCGATCATT CGGTAGAGCA GGCAAAACCT TCCACAAGCA AGACCACATC CAAAACCAAC 
GATAGAAAGA CTGAAGATGA AGTACTTTAC GCTCAACTTG CTCAAAATGC CATCCGAAAC 
CAACAACCAA GAAAGAACAA TACTGGAGTC CAAATAGCGC CAGAGTTGTC AGATATTGTC 
ATTTACATGC AAGCGACAAA ATTCAAAGGA TTTCCCCCAG TCGATGGCAT TCAATCACCA 
AGGATAATGG AGGAAGGGCC AGCTTCTGCA AGTCTTTCAT TTTCATCACG GGCTCGTACG 
CCATCAAATC TGCTGAATAC ACCAGCCCCT CCACGACGAC AGCGAAGTAG CACTCAGTTG 
AGTCAAGAAC TGGCTGCAGA ATTTTTGGGA TCAGTTAGGG CAAATGCTAC AGCTACATGC 
TACCAGGTTA CATCTCTTAA TGAAAACGCT GCAAAGAAAC TGATGAAAAG ACATCCAGCA 
AAATGCGTAT CTTATACTCG GGACCATCTG ATTCGTACGT ATCCAAGTGC CAAGCACTAT 
GACTCGTCCA ACTTTAACCC CATCAACTGC TGGGCTCATG GAATGCAAAT GGTTGCTTTA 
AATTTTCAAA CTCCTGATGT TATAATGGCA GTTAACCAAG CGATGTTCGA GCAATCTGGA 
AACTGTGGAT ATCAACTGAA ACCACGTTGT CTTTGGGATG AATCTCATCT TTTGTACAAC 
AAATTTTTGC CTCTCTCCAA AGACATTGCC GGCCATTCTG CTTTGTTACT AAACTTAACT 
ATTATATCCG GACAGCACGT GTATCCAAAC ACCCATTACG CCAGTTTATA TGTCGAAATT 
GAAGTCATCG GTATTCACAA CGACTGTGTT CGTGAGAAAT CAAAAGTGGT CCAGAGAAAT 
TCTGTGAATC CAATCTGGAA CCACACCACT CAACTTCGAA TAGCTTGTGT TGACTTAGCA 
TTCCTTCGCA TAGCAGTATG TGATAGTGGG CAAAATGGAA GAGTCGTTGC TCACCGAGTT 
GTTCCTGTCA AATGCATTCG TCCGGGTTTC CGACATCTCC CACTCAGAAC TCCAACTAAT 
CTTCCGATCG ACAACGCTAT GATATTTTTA AGAACAAGAT TCGAGCAAGA AGAACACATC 
TACCTCCACG ACGATGATTC AAACACCTAT TGTAATCTAG AACATACGCT GGCATACCGC 
ACAGACTTAA CACCCAATTT ATCTCCAACG CCAATTTTGA AGAAACAGAT CTTTGTTCTT 
CGAATTACAG GCGCGTTTGC TGATGAAACA GCAATTACAG TGCACAGCGA GTCAGGAAGT 
ACAGTGAAGA CGGTGATGCA ACAGGCGCTG CTTAACGCTG GAAAGAATGC GGATCAGGTT 
GAAGAGTATG TACTCATCGA AGAATCACTG CCGGCACCTT CTGGGGAAGA TCCAATTGAG 
CAGAGAGTAC TTCCTCTGAA CGAGCCTATC ATGGACGCCG TGGCCTGCTG GAACGGTTCA 
ATGCGTCGTT TTGTGTTACG AAAAAAAGGC AGTGACCCAT CATCTAGAGC TTGGATCACT 
TCAATTATAA AATCCGGAAC AAGTGGAAGC TCCACGTCAG TCTCACCAAG TCCGTTGACC 
AAAGATGGAC ACGTAAAGAG TGCATCAAGC AATCAACTTC ATGGACGATC ATTGGACACT 
GACGCTTTTG GAGAGCACCT TGAAGTGACA GAAGGGTTGA ACCCTCGTGC AAGATCAATG 
GGCGACACAT TTCTGGTATG TGTTCACAAT GTTAGTGAAG ATCAGCCTTA TGCAATCCTA 
CGTGCTGGAA TCCACAGCAC TGCCGCCGAC ATTATTCGTC AGGTGTTTGT TAAAGCTCGC 
CGTTCAAATG TAGACGATTC CGAGTTTGTT CTTGTTGAAG AAACCTGCGA TGATCCAAAA 
CTCAATCAAG GCCAAATGAC TCCAAAGTAT CCAAACAACC GCACCACAAG CCGAGTGTTA 
GGACAAAACG AAAATGTTTG GAAAGCACAA TCGAGGTGGA AGTCAATGGG CAGATTTGTA 
CTTGAAAACC GAAAGGACAC AGTGCACGCA ACCCTCGAAA AAGTGAGATC ATTTATATCA 
AAACTGGAAG CTGCAAAAGC AAGCATGGAT CCCAGACACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360638.1
CDS	1..10665
Translation	MSLRQASHKFPSVDSMPTSRDSSMETTDRYPFGYTTELARTEPSASTAKRLRQRLQRQQSGQSGLSGRRYTGESSECSSLVGTPTDTGRSFTFGAAQRVMRERDDRKPSVTQNLSEIMLEAIHYEDVNLVERLLTAHSSKQPMSTSPSIGSTNTLTDLAQRRHGAGAHRRSNASSTVSTHSGGRSTCAMMNTLHMAVAHKQRDIVELLLKNGYDPNTPASCHCKGNCTATGNIPLTSIIPRTHSMTPELCSTCSQLRVVSIVDQTPLGVAVRSQSSELIALLIAYGGDVNLGDEDGNTPLMLAVRESPLSWPCLHTLIFFGAQIEQKNMRGICPLDLAPELKKLQQTCVEELFKNACSGDCEPPSRSTPGPSAKNWNRLQVDNVSQGERSLSKAPLSPKPSAAASVSTCSMLETSSAKECARRKSLVSLQLHRKSRNVKEYPVIDCVSWEQAWELLKKMACNPECLDNIVNSLSKFATQMENTPSSVDRDQFDAHMGGLLHKMIHTAIEEYESSTPTYKKQKKLHLTWLLSQIATFCFTFLQKSGTSRQFSALNTLNKIIDAGLVHDLFAFNDVVFHSSRILNRCHAFDSDEPPTPSGESFNSNADQNSTVDHVFIYGAGYKDQSLLPSPTVAPTRHKSDLMNSFSEMDPSQVIVALHNAITMQNREVGSRSVCSPAHRWRQCCAHCTQILVARLLLFLTHAKKFRSRLSERHQLRSLVALLEPTLEPQLLCLLLQVLALIALDPSTHTTLIDIQIDDVLIQMLLPADDWYYTNHSTKYGTFVKYHAARVLVYVGMGDRVGSRVNLFNMMTFEPSAKSGSQQNEDDYICETCATPRSRSTFSRSAVSVEGVLLKVLAEVAELVRQSQHLSASEPITEESPSAGSPPAVLPNQAEQKSINEKNAEKLTQLGCQILISLEQLEAHLCKLGLVLDSVLLLRLLLHKLSWDLGLVTKKRVAVVDHVYKPITDPRAHSSCSLGNNRNFGKSKSFDRREDSKRDKNYLRVDQGSRASKRVHIRRSSSVEIIRPKRLSSGAKDIKNRERRKRLGTDTSSGSNRSKKTNSSSSSVQKHLPKYIQSLFRSRMGTDPCKRTSRRDSNESNTSGSDAVLEFTRKLQNYPLTRREAQRLTYRSNGQQDSNDSKMGPRTLGYVYLPELEIQGASPPRSPGPLTGNSLEEGTTMLLDTRRPSSPQAIPGLPLIEIRRPSALSQFEFGYFVNSPELSGSEVSDCAPLLLSGQIGGSRKSSDETSLGGWSSRASSVMSQRSSRSSVGLRLSTFSGGTSIASDNSGPFLFSFVLRKRASTIGTRIPIPRRALSRSSGDSLRVPDRESPLHLASMTEMNPDFQCVRQLILNLLNVYTKRNDNVVSTMKECADVLRQILNSPQHPTVKNWCAEIIHVVSTHVEEEEPTTAENNEQINDEYLEFQDQVISGSLPCPKEEAAYLASIQLSVEEQWPSNKRTQTIRRHLLKGQFGRIRDLAQKIMVTPWEVDQNLYCTPPRFPNESANASRAQSVVEEIQHRSRTPTLLRCITNTDGLMSEEMQAQCLPVDLRGDRRTIKLVKERKRKLFHSQVYESEIGMKKLYIQTAKKLAAFGCKVFQVKELLHGRTLRKTLRLLCLSSAQLCLLDGSTKLVLKRQHASTLQQWRVGGGVSKHQLLLEFRGTKWQLIAPSYNALKSISMTLWEIMQNSASNSIQRSLNQSMHRSHTEATVSTSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKVRSFISKLEAAKASMDPRHE

Target ORF information:

RefSeq Version	NM_001373495.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373495.1

ORF Insert Sequence:

ATGTCCTTAC GCCAAGCATC ACACAAATTT CCATCGGTGG ACTCAATGCC AACATCACGG 
GATTCATCGA TGGAAACAAC CGACCGGTAC CCGTTTGGGT ATACGACCGA ATTGGCACGT 
ACCGAACCAT CTGCATCAAC TGCCAAACGT CTTCGACAAA GGTTACAGCG ACAGCAAAGT 
GGTCAATCAG GATTATCCGG TCGACGGTAT ACTGGAGAAA GCAGCGAGTG CTCATCACTT 
GTCGGAACAC CCACAGACAC TGGCAGATCA TTTACATTTG GAGCAGCACA AAGAGTCATG 
CGGGAACGGG ATGACCGCAA ACCATCAGTT ACCCAGAACT TGTCAGAAAT AATGCTCGAG 
GCAATACATT ATGAAGATGT GAACTTGGTG GAAAGGTTGC TCACTGCGCA TAGTTCAAAA 
CAACCAATGT CAACATCTCC GTCAATTGGT AGCACAAACA CATTGACGGA TCTTGCTCAA 
CGTAGACACG GTGCGGGGGC ACACCGACGT TCCAATGCCA GTAGTACGGT GTCCACACAT 
TCCGGAGGAA GATCCACGTG TGCTATGATG AACACGTTGC ACATGGCTGT GGCTCATAAA 
CAACGAGATA TAGTGGAACT TTTGCTTAAA AACGGCTACG ATCCCAATAC TCCCGCATCG 
TGTCACTGTA AGGGAAATTG CACGGCGACT GGGAATATTC CACTGACTTC GATCATTCCG 
AGAACTCATT CAATGACGCC AGAATTGTGT TCGACGTGCT CTCAACTGAG GGTTGTGTCA 
ATCGTGGATC AAACACCGCT TGGTGTGGCA GTCAGATCAC AGTCCAGCGA ATTGATAGCA 
TTGTTGATTG CCTACGGAGG AGATGTTAAT TTAGGTGATG AAGATGGCAA CACGCCGTTG 
ATGCTAGCCG TACGAGAATC ACCTCTGTCT TGGCCTTGCC TGCACACGCT CATTTTCTTT 
GGAGCTCAAA TTGAACAAAA GAACATGCGC GGAATATGCC CTCTTGATCT TGCTCCAGAG 
CTGAAGAAAC TTCAACAAAC TTGCGTTGAA GAGCTTTTCA AAAATGCGTG CTCGGGTGAT 
TGTGAACCAC CATCTCGATC TACACCGGGA CCATCAGCCA AAAACTGGAA TAGACTACAA 
GTTGACAATG TTTCCCAAGG AGAACGATCA CTGTCAAAAG CTCCATTGAG CCCAAAACCG 
TCTGCTGCAG CAAGTGTCTC AACGTGTAGC ATGCTGGAAA CCTCATCAGC AAAAGAATGT 
GCACGACGAA AATCATTGGT GTCGCTTCAA CTTCACAGAA AAAGCCGAAA CGTGAAAGAA 
TATCCTGTAA TTGACTGCGT CAGTTGGGAA CAAGCATGGG AATTGCTGAA GAAGATGGCA 
TGTAATCCAG AATGTCTAGA TAACATCGTG AATTCATTGT CAAAATTTGC AACCCAAATG 
GAGAACACCC CGTCAAGCGT TGATCGTGAT CAATTCGATG CACACATGGG TGGCCTACTA 
CATAAAATGA TTCATACAGC GATTGAGGAG TACGAGTCTT CGACACCAAC CTACAAAAAG 
CAGAAGAAAC TCCATCTCAC CTGGCTGCTC AGCCAAATTG CCACGTTTTG TTTTACATTT 
CTGCAGAAAA GTGGAACGAG CCGACAATTC TCAGCACTGA ACACGTTGAA TAAAATTATT 
GATGCTGGTT TAGTACATGA TTTGTTCGCA TTCAACGACG TTGTCTTTCA TTCTTCAAGA 
ATTTTGAACC GATGCCATGC ATTTGATTCC GACGAACCAC CAACTCCATC TGGCGAATCT 
TTCAACTCAA ATGCAGACCA AAACTCAACT GTCGATCATG TTTTCATTTA CGGAGCTGGC 
TATAAAGACC AATCGTTATT ACCTTCTCCT ACGGTGGCAC CAACTCGACA CAAGTCCGAC 
TTAATGAATT CTTTCAGCGA AATGGATCCA AGCCAGGTCA TTGTGGCCTT GCACAACGCG 
ATAACAATGC AAAACCGGGA AGTAGGGTCA AGATCTGTTT GTAGTCCGGC TCATCGATGG 
AGGCAGTGTT GTGCTCATTG TACACAGATA TTGGTGGCCA GATTATTGCT ATTCTTAACA 
CACGCAAAAA AATTTCGAAG TCGGTTGTCT GAACGACATC AGCTCAGAAG CTTGGTAGCT 
TTGTTAGAAC CAACTTTGGA ACCTCAACTC TTATGTCTTT TACTTCAAGT TCTAGCTCTT 
ATAGCGTTAG ATCCATCTAC CCACACAACT TTGATAGACA TTCAAATTGA CGACGTCTTG 
ATTCAAATGC TACTCCCAGC AGATGATTGG TACTACACTA ATCACAGCAC TAAATATGGA 
ACTTTTGTAA AGTACCATGC TGCTAGAGTT TTAGTGTACG TTGGAATGGG TGATCGAGTA 
GGAAGTCGAG TCAATCTGTT CAACATGATG ACGTTTGAGC CATCTGCAAA AAGCGGGAGT 
CAACAAAACG AGGACGACTA CATTTGTGAG ACATGTGCCA CACCGCGCAG CCGAAGTACA 
TTTTCAAGAT CAGCAGTTTC AGTGGAAGGT GTTCTGTTAA AAGTGTTGGC AGAAGTTGCT 
GAGCTTGTAC GTCAGAGTCA ACACCTATCA GCAAGTGAAC CAATAACTGA AGAATCGCCC 
AGTGCAGGAT CTCCACCCGC TGTCCTACCA AATCAGGCTG AACAGAAAAG TATTAACGAA 
AAAAATGCAG AGAAACTTAC ACAACTTGGG TGCCAAATTT TGATCTCATT GGAACAACTT 
GAAGCTCATT TATGCAAACT TGGACTGGTA CTTGATTCGG TTCTACTTCT CCGTCTACTT 
CTTCATAAGT TGTCATGGGA TCTTGGATTA GTCACAAAAA AGAGGGTTGC TGTGGTGGAT 
CATGTTTACA AACCAATCAC GGATCCGAGA GCTCATTCTA GCTGCAGTCT TGGAAACAAC 
AGAAACTTTG GTAAATCTAA ATCTTTTGAT AGGCGAGAAG ATAGCAAACG GGACAAAAAT 
TACTTGCGTG TTGATCAGGG ATCTCGTGCG TCAAAGAGAG TGCATATCAG ACGATCTAGT 
TCAGTAGAGA TTATCAGGCC AAAACGACTC AGTTCCGGAG CAAAAGATAT CAAAAATCGA 
GAACGGAGGA AAAGGCTGGG AACAGATACA AGTAGCGGGT CGAATCGTTC CAAGAAAACC 
AACTCCTCGT CTTCATCTGT TCAGAAACAC TTGCCAAAAT ACATTCAATC ATTATTCCGA 
AGTCGGATGG GAACTGATCC ATGCAAGAGG ACATCGAGAA GAGACTCTAA TGAGAGTAAT 
ACAAGTGGAA GTGATGCTGT TCTTGAGTTC ACAAGAAAAC TTCAAAATTA TCCGTTGACG 
AGACGCGAAG CGCAAAGATT GACGTATCGA AGCAATGGGC AACAAGATTC AAATGACTCG 
AAGATGGGAC CACGAACTTT AGGATACGTT TACCTCCCAG AACTAGAAAT TCAAGGTGCA 
AGTCCGCCAA GATCACCGGG ACCTCTAACT GGAAACTCGT TAGAAGAAGG AACCACAATG 
CTTTTGGACA CAAGACGGCC GAGCAGCCCA CAAGCAATCC CCGGCCTTCC TTTAATTGAA 
ATTCGTCGGC CCTCAGCTCT CTCTCAATTT GAATTTGGAT ACTTTGTAAA CTCACCAGAA 
TTATCAGGAA GTGAAGTATC TGATTGTGCC CCATTACTTC TCAGTGGTCA AATTGGTGGA 
AGCCGAAAAA GCTCCGACGA AACATCATTG GGAGGATGGT CCAGCCGTGC TTCATCGGTA 
ATGAGTCAAA GGTCGTCCAG ATCCAGTGTT GGCTTGAGGT TATCAACATT TTCTGGAGGG 
ACGAGTATTG CTTCGGATAA CTCTGGGCCA TTTTTGTTCA GTTTTGTTTT GAGAAAACGT 
GCATCTACTA TAGGAACTAG AATACCAATA CCTAGGAGAG CTTTGTCAAG ATCTTCAGGA 
GACAGTCTGA GAGTACCTGA TAGAGAAAGT CCACTTCATT TAGCAAGTAT GACAGAGATG 
AATCCGGATT TTCAATGTGT TCGACAGCTC ATCTTGAACC TTTTGAATGT TTACACGAAA 
AGAAATGACA ATGTGGTTTC TACAATGAAG GAATGTGCGG ATGTTCTTCG TCAAATCCTA 
AACTCTCCTC AACATCCAAC TGTCAAGAAT TGGTGTGCCG AAATAATTCA TGTAGTCAGT 
ACTCATGTAG AAGAAGAGGA ACCAACGACG GCAGAAAATA ATGAACAAAT TAATGACGAG 
TATCTGGAAT TTCAAGACCA AGTGATATCA GGAAGTTTAC CATGCCCGAA GGAAGAGGCT 
GCATACCTTG CAAGTATTCA GTTATCAGTT GAAGAGCAGT GGCCGAGTAA TAAAAGGACC 
CAGACAATTA GAAGACATTT ATTGAAAGGA CAGTTTGGTC GAATCCGGGA TCTCGCCCAA 
AAGATCATGG TGACTCCATG GGAAGTTGAT CAAAACTTGT ACTGCACACC ACCCAGATTT 
CCCAATGAAT CGGCAAATGC TAGTAGAGCA CAATCAGTGG TTGAGGAAAT TCAGCACAGA 
TCAAGAACTC CAACTCTTCT GCGATGTATC ACAAACACAG ACGGCTTAAT GTCAGAAGAG 
ATGCAAGCTC AGTGCCTTCC CGTCGATCTA CGTGGTGATC GTAGAACTAT CAAGCTGGTC 
AAGGAGAGGA AGCGAAAATT GTTCCATAGT CAAGTCTATG AAAGTGAAAT TGGCATGAAG 
AAATTGTACA TTCAGACCGC CAAAAAACTA GCCGCATTTG GTTGTAAGGT TTTTCAAGTT 
AAAGAGTTGC TTCACGGACG AACACTCAGA AAAACTCTTC GACTGCTTTG CCTTTCTTCT 
GCTCAATTGT GTCTTCTCGA CGGATCAACA AAACTTGTGC TTAAACGGCA GCACGCATCG 
ACGTTACAAC AGTGGAGAGT AGGTGGTGGA GTTAGTAAGC ATCAACTATT GCTCGAATTC 
CGTGGAACCA AGTGGCAACT TATTGCACCG TCGTATAATG CACTGAAATC GATATCGATG 
ACATTATGGG AGATTATGCA AAATTCTGCT AGCAATTCAA TCCAGCGGAG TCTCAACCAG 
TCAATGCATC GTTCGCACAC CGAGGCGACT GTCTCAACAT CACGTAGTGA CTCAACCTCT 
TCAACGCAAT CAGCCACAGC TGGATTCATT CTCAATGAAG AACCAATCAC ACTGTTCCGA 
CTTGAACTCG AACGACTTCA ATATATTCTT CATTTCCCTG AAGAAGTTGC ATTCCAACTA 
TCTTCTACTG AGTATCAGCT TTTCTACTCA ATACAACCCA TGGATTATGT TCGTTACGTC 
AGTTGCGATC TCACATCTGT TCCAGTGTCA GAAAATCCAA GTCCAGTGCG AAATCTTGTG 
AAAAGGCTAT CAGAGGTATC ATCCTGGATA ACACATGTTA TTGTCAGTCA GCCAACACAT 
GACGATCGAA AGGTTGCTCT TACTGCAATT TTGAGAATTG TGGAAACTTG CTGGAATATT 
GGAAACTTTA ATGCAGCTGT TGAGGTTCTT ATGGGTTTAA AGAGTGAAAA ACTCAGACCA 
TTCTGGCTTT CTTTGCGACA AGAGGAAAAG TCTCAGTTTG ATTCGCTTTG CGAAACCTTG 
CTTCCTGCAA ACCAAGCTCT CCCATCTCAA GCTTACATCA ACGCGGTCCA ACGAGCACTG 
AGAATGCCAC AATCTCGTGT GATTCCATTT TTTGGAATAT TTCTTCGTGA TCTCTACGCA 
ATTGTGAATG ACTTGCCGAA TATCGTTGTG ATTGGACAAG AAGGTGAAAC CCAAAAGCTG 
GAATTCATGA GCGATCCCAA TGGCGAAGAT CATTTTTCGT CGAGAATTGG AGTGGGTGGG 
CTGTTGAATG CTGATAAAAT CAACTTGGTG GCAATTGTTT TGGACAACTT GGAATTATTC 
CATCGTCACA GCAGGACAAT GATCAAATTG TTAGAAGAGC AAGCAGTCCC ACCAATTCAA 
ATTCCTCAGA ACGAGCGGGA GCAAAAAGAA AAGGAAGCAA AAACATATGA ACCAGTACAA 
GTGGTTCGCG GCTCTTCTCA TGGAGTGGCT CTCATCCCTC TTGATACCCT AACATTCGAT 
CTTGATGTTA TCCAACGACT CCAGCACGGA ACAACAGTGA TCCATTACGA ACCTGATTCG 
GGAAGAAGCA ATTTGTGTCT GCTGCGGTTA GACCCTAGTT GCGGTCAAAT AAACTGGCAT 
AAGATTTCAT ATTCAGTTAA CAAAGATCCC AAAGAAAAGG ATGTGCTCGC CAAAGTATCC 
GTTTCAAATC TGCAGCCGCT AGACTCTGGC CGAGGGGCTC CGTCACCAAT GCCATCTGGA 
AGAACACCAG GAACTGGAGG AGTTGGAGTA GAGGAGGGAG AACTCAAATT AAGTGTTGTG 
AAAGGAGTCG AACTAGTTGA TAGTTATGAT ATTGATATTG AGGCAATTTA CCGTCGTCAT 
TCTATGGAAG AGATGTCAGT TCCTGTTAGC TGTTGGAAAG TTAGTCATGG TCAGTTGTTA 
TCGGATAATG AGTTCATCTA CTTTTTGGCC CCTCAACAAA TTGCTCAATT TTGGACCAAC 
GGGTTGCAAT CTGTAGTTAA AAGTCTCCAA GGACAGCAAA GGTACCCTGA TCGGCGAATG 
TTATGGATCA AAAATGTCTA CTTATCACTT TACGAGATAA CGGGCGAATC AAACTGTGGA 
CCACGGCCAT TTGAAGCACT TCAGGCATTT GGGCTGAGTC AAACAAATAC TAACGCAACA 
CGACCCAATG ACTCGTCGCT GTCCAGTGAG CCAGGAGGAG CTAAAAGCAG ACTGAAGAAC 
CTTAAAAATG CTATGCAAAA GAAGCTTCGT GGTGCATCGC GCGAAGGAAG CAGATCTCAG 
TCACCTCAAC CTCATTCTCC TCTTGTCCGA CCACCATCAA TAAAATCGCA AATATCGTCA 
CAATCTGGTC CGCCGGGTCC GAACTCTCCA GGCTATTTGC TGAAACCACG TGGAGAACCT 
GCCAATAGTG ATGCAGGAGA CATTGATAGC ATTTATACAC CTCGATCAAG AACTCCAACA 
AGCAGCTCCT ATGGAGGGCG ATCCGTTGGA GGGAGATCAT GTAAATCGTG GAGATCAAGA 
GGAGGAGAGA CACCAAATTC GGGTTCCATA AGCAGCTCGG GTCAGATGTC AATTCAAGTG 
TCTGGTCTGA GTGGACCATC GGGAAAAGAG TTTCAGGAAA AACCATTAAC ACTTGTAGAG 
TTCGCTGAAC TTTTTCGATT GTTCAATACA AGAATGAGAA AAGATTTGAG AGATGTGTTC 
AATGATGTCC TGTCTACGGC AACTACTCCT CAGCATTGTC CTAAAAGAGA ACGTGATCGC 
CACAGTCCGA GAATGCAGTC AAGGCTGGCG AGTGTCTCCA ACTCGTACAA TGCAGATTTT 
CTGTCTAACG ATTTCTTGAC TCGGAATACG GCAGTAACGT CCCATCATAT CAGTGAAAAG 
CAAAACAAAA TCTACAATGC ATTGGCACTT GCGAGTGTTA ATAGCATGGG CGGACTCATG 
GACACTTCCC GGTCGTCGAT GCTGACGCCG CAAATGCTGC GCGCTTTTGT CAACACTCAT 
CAGATGGAGC AGATTGACGA GCAAACGGCG ATTAAGCTTA TTCAGGATCA TGAACCTGAT 
GGTATCTGTC GACAAAAGAA TCAAATGTCA TTCGAAGGAT TCACTAGATT CCTTTGCGAT 
CCTGTCAACT TTGCATTCGT ACCGGAAACA ATTGAACCAG ATGAAGAGGA CCTACGGTAC 
CCGTTGTCTC ATTATTACAT CAATTCAAGT CACAACACCT ATTTGACGGG ACATCAACTC 
AAGGGACCCA GTAGCTCGGA GATGTACAGA CAGGTTCTCC TCACCGGGTG TCGGTGTGTC 
GAGCTTGATT GTTGGGATGG AGATGACGGA TTGCCATTGA TTTATCATGG ACACACATTG 
GTTAGCAAGA TTGGGTTTAG ACAGGTGGTC GAAATTATCA AAAAATCTGC ATTCATAACA 
TCGGATCTTC CTGTGATATT ATCAATTGAG AATCATTGTT CCCTACAACA ACAGGCGAAA 
ATGGCACAGA TGTTCAAAAC AGTTCTTGGA GATTTACTAG TATCAAACTT CCTTTTTGAA 
GCTGATTTCT CGGACTCTCC ACGTCTTCCA TGCCCTCTCC AAATGAAAAA TAAGATCTTG 
ATAAAAAATA AGAAAATGAT AGTCGACCCA CCAACTCCAT TGCCAATGAT TGAACGAGGA 
GCTGTGCAGC GTGGTGAAAC TCAATTAAAT CTTCACAGAA AACAGAGTAA AAACTCCTAC 
GAAAGCAGTA CGGTAGATGA AGTGGAAGAT GACGACCTAG ATGAGTTTCT TGACGATGAA 
GAAAATGAGG AGGATGACCA AGAAGAGGTT CAAGTCAGAA GTGAAAAAGA AGACAGTCCG 
AAGACATCAA AACGTGCAGA GAAATCGGCT AGAAACATCA AACAGCAAGA TTCGTTATGT 
AGCGATCATT CGGTAGAGCA GGCAAAACCT TCCACAAGCA AGACCACATC CAAAACCAAC 
GATAGAAAGA CTGAAGATGA AGTACTTTAC GCTCAACTTG CTCAAAATGC CATCCGAAAC 
CAACAACCAA GAAAGAACAA TACTGGAGTC CAAATAGCGC CAGAGTTGTC AGATATTGTC 
ATTTACATGC AAGCGACAAA ATTCAAAGGA TTTCCCCCAG TCGATGGCAT TCAATCACCA 
AGGATAATGG AGGAAGGGCC AGCTTCTGCA AGTCTTTCAT TTTCATCACG GGCTCGTACG 
CCATCAAATC TGCTGAATAC ACCAGCCCCT CCACGACGAC AGCGAAGTAG CACTCAGTTG 
AGTCAAGAAC TGGCTGCAGA ATTTTTGGGA TCAGTTAGGG CAAATGCTAC AGCTACATGC 
TACCAGGTTA CATCTCTTAA TGAAAACGCT GCAAAGAAAC TGATGAAAAG ACATCCAGCA 
AAATGCGTAT CTTATACTCG GGACCATCTG ATTCGTACGT ATCCAAGTGC CAAGCACTAT 
GACTCGTCCA ACTTTAACCC CATCAACTGC TGGGCTCATG GAATGCAAAT GGTTGCTTTA 
AATTTTCAAA CTCCTGATGT TATAATGGCA GTTAACCAAG CGATGTTCGA GCAATCTGGA 
AACTGTGGAT ATCAACTGAA ACCACGTTGT CTTTGGGATG AATCTCATCT TTTGTACAAC 
AAATTTTTGC CTCTCTCCAA AGACATTGCC GGCCATTCTG CTTTGTTACT AAACTTAACT 
ATTATATCCG GACAGCACGT GTATCCAAAC ACCCATTACG CCAGTTTATA TGTCGAAATT 
GAAGTCATCG GTATTCACAA CGACTGTGTT CGTGAGAAAT CAAAAGTGGT CCAGAGAAAT 
TCTGTGAATC CAATCTGGAA CCACACCACT CAACTTCGAA TAGCTTGTGT TGACTTAGCA 
TTCCTTCGCA TAGCAGTATG TGATAGTGGG CAAAATGGAA GAGTCGTTGC TCACCGAGTT 
GTTCCTGTCA AATGCATTCG TCCGGGTTTC CGACATCTCC CACTCAGAAC TCCAACTAAT 
CTTCCGATCG ACAACGCTAT GATATTTTTA AGAACAAGAT TCGAGCAAGA AGAACACATC 
TACCTCCACG ACGATGATTC AAACACCTAT TGTAATCTAG AACATACGCT GGCATACCGC 
ACAGACTTAA CACCCAATTT ATCTCCAACG CCAATTTTGA AGAAACAGAT CTTTGTTCTT 
CGAATTACAG GCGCGTTTGC TGATGAAACA GCAATTACAG TGCACAGCGA GTCAGGAAGT 
ACAGTGAAGA CGGTGATGCA ACAGGCGCTG CTTAACGCTG GAAAGAATGC GGATCAGGTT 
GAAGAGTATG TACTCATCGA AGAATCACTG CCGGCACCTT CTGGGGAAGA TCCAATTGAG 
CAGAGAGTAC TTCCTCTGAA CGAGCCTATC ATGGACGCCG TGGCCTGCTG GAACGGTTCA 
ATGCGTCGTT TTGTGTTACG AAAAAAAGGC AGTGACCCAT CATCTAGAGC TTGGATCACT 
TCAATTATAA AATCCGGAAC AAGTGGAAGC TCCACGTCAG TCTCACCAAG TCCGTTGACC 
AAAGATGGAC ACGTAAAGAG TGCATCAAGC AATCAACTTC ATGGACGATC ATTGGACACT 
GACGCTTTTG GAGAGCACCT TGAAGTGACA GAAGGGTTGA ACCCTCGTGC AAGATCAATG 
GGCGACACAT TTCTGGTATG TGTTCACAAT GTTAGTGAAG ATCAGCCTTA TGCAATCCTA 
CGTGCTGGAA TCCACAGCAC TGCCGCCGAC ATTATTCGTC AGGTGTTTGT TAAAGCTCGC 
CGTTCAAATG TAGACGATTC CGAGTTTGTT CTTGTTGAAG AAACCTGCGA TGATCCAAAA 
CTCAATCAAG GCCAAATGAC TCCAAAGTAT CCAAACAACC GCACCACAAG CCGAGTGTTA 
GGACAAAACG AAAATGTTTG GAAAGCACAA TCGAGGTGGA AGTCAATGGG CAGATTTGTA 
CTTGAAAACC GAAAGGACAC AGTGCACGCA ACCCTCGAAA AAGTGAGATC ATTTATATCA 
AAACTGGAAG CTGCAAAAGC AAGCATGGAT CCCAGACACG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plc-1 ( NM_001373491.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001360639.1 Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.

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CloneID	OCa851226
Clone ID Related Accession (Same CDS sequence)	NM_001373491.1
Accession Version	NM_001373491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	PhosphoLipase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). COMPLETENESS: incomplete on the 5' end.

ATGTCCTTAC GCCAAGCATC ACACAAATTT CCATCGGTGG ACTCAATGCC AACATCACGG 
GATTCATCGA TGGAAACAAC CGACCGGTAC CCGTTTGGGT ATACGACCGA ATTGGCACGT 
ACCGAACCAT CTGCATCAAC TGCCAAACGT CTTCGACAAA GGTTACAGCG ACAGCAAAGT 
GGTCAATCAG GATTATCCGG TCGACGGTAT ACTGGAGAAA GCAGCGAGTG CTCATCACTT 
GTCGGAACAC CCACAGACAC TGGCAGATCA TTTACATTTG GAGCAGCACA AAGAGTCATG 
CGGGAACGGG ATGACCGCAA ACCATCAGTT ACCCAGAACT TGTCAGAAAT AATGCTCGAG 
GCAATACATT ATGAAGATGT GAACTTGGTG GAAAGGTTGC TCACTGCGCA TAGTTCAAAA 
CAACCAATGT CAACATCTCC GTCAATTGGT AGCACAAACA CATTGACGGA TCTTGCTCAA 
CGTAGACACG GTGCGGGGGC ACACCGACGT TCCAATGCCA GTAGTACGGT GTCCACACAT 
TCCGGAGGAA GATCCACGTG TGCTATGATG AACACGTTGC ACATGGCTGT GGCTCATAAA 
CAACGAGATA TAGTGGAACT TTTGCTTAAA AACGGCTACG ATCCCAATAC TCCCGCATCG 
TGTCACTGTA AGGGAAATTG CACGGCGACT GGGAATATTC CACTGACTTC GATCATTCCG 
AGAACTCATT CAATGACGCC AGAATTGTGT TCGACGTGCT CTCAACTGAG GGTTGTGTCA 
ATCGTGGATC AAACACCGCT TGGTGTGGCA GTCAGATCAC AGTCCAGCGA ATTGATAGCA 
TTGTTGATTG CCTACGGAGG AGATGTTAAT TTAGGTGATG AAGATGGCAA CACGCCGTTG 
ATGCTAGCCG TACGAGAATC ACCTCTGTCT TGGCCTTGCC TGCACACGCT CATTTTCTTT 
GGAGCTCAAA TTGAACAAAA GAACATGCGC GGAATATGCC CTCTTGATCT TGCTCCAGAG 
CTGAAGAAAC TTCAACAAAC TTGCGTTGAA GAGCTTTTCA AAAATGCGTG CTCGGGTGAT 
TGTGAACCAC CATCTCGATC TACACCGGGA CCATCAGCCA AAAACTGGAA TAGACTACAA 
GTTGACAATG ATCCTGTAAT TGACTGCGTC AGTTGGGAAC AAGCATGGGA ATTGCTGAAG 
AAGATGGCAT GTAATCCAGA ATGTCTAGAT AACATCGTGA ATTCATTGTC AAAATTTGCA 
ACCCAAATGG AGAACACCCC GTCAAGCGTT GATCGTGATC AATTCGATGC ACACATGGGT 
GGCCTACTAC ATAAAATGAT TCATACAGCG ATTGAGGAGT ACGAGTCTTC GACACCAACC 
TACAAAAAGC AGAAGAAACT CCATCTCACC TGGCTGCTCA GCCAAATTGC CACGTTTTGT 
TTTACATTTC TGCAGAAAAG TGGAACGAGC CGACAATTCT CAGCACTGAA CACGTTGAAT 
AAAATTATTG ATGCTGGTTT AGTACATGAT TTGTTCGCAT TCAACGACGT TGTCTTTCAT 
TCTTCAAGAA TTTTGAACCG ATGCCATGCA TTTGATTCCG ACGAACCACC AACTCCATCT 
GGCGAATCTT TCAACTCAAA TGCAGACCAA AACTCAACTG TCGATCATGT TTTCATTTAC 
GGAGCTGGCT ATAAAGACCA ATCGTTATTA CCTTCTCCTA CGGTGGCACC AACTCGACAC 
AAGTCCGACT TAATGAATTC TTTCAGCGAA ATGGATCCAA GCCAGGTCAT TGTGGCCTTG 
CACAACGCGA TAACAATGCA AAACCGGGAA GTAGGGTCAA GATCTGTTTG TAGTCCGGCT 
CATCGATGGA GGCAGTGTTG TGCTCATTGT ACACAGATAT TGGTGGCCAG ATTATTGCTA 
TTCTTAACAC ACGCAAAAAA ATTTCGAAGT CGGTTGTCTG AACGACATCA GCTCAGAAGC 
TTGGTAGCTT TGTTAGAACC AACTTTGGAA CCTCAACTCT TATGTCTTTT ACTTCAAGTT 
CTAGCTCTTA TAGCGTTAGA TCCATCTACC CACACAACTT TGATAGACAT TCAAATTGAC 
GACGTCTTGA TTCAAATGCT ACTCCCAGCA GATGATTGGT ACTACACTAA TCACAGCACT 
AAATATGGAA CTTTTGTAAA GTACCATGCT GCTAGAGTTT TAGTGTACGT TGGAATGGGT 
GATCGAGTAG GAAGTCGAGT CAATCTGTTC AACATGATGA CGTTTGAGCC ATCTGCAAAA 
AGCGGGAGTC AACAAAACGA GGACGACTAC ATTTGTGAGA CATGTGCCAC ACCGCGCAGC 
CGAAGTACAT TTTCAAGATC AGCAGTTTCA GTGGAAGGTG TTCTGTTAAA AGTGTTGGCA 
GAAGTTGCTG AGCTTGTACG TCAGAGTCAA CACCTATCAG CAAGTGAACC AATAACTGAA 
GAATCGCCCA GTGCAGGATC TCCACCCGCT GTCCTACCAA ATCAGGCTGA ACAGAAAAGT 
ATTAACGAAA AAAATGCAGA GAAACTTACA CAACTTGGGT GCCAAATTTT GATCTCATTG 
GAACAACTTG AAGCTCATTT ATGCAAACTT GGACTGGTAC TTGATTCGGT TCTACTTCTC 
CGTCTACTTC TTCATAAGTT GTCATGGGAT CTTGGATTAG TCACAAAAAA GAGGGTTGCT 
GTGGTGGATC ATGTTTACAA ACCAATCACG GATCCGAGAG CTCATTCTAG CTGCAGTCTT 
GGAAACAACA GAAACTTTGG TAAATCTAAA TCTTTTGATA GGCGAGAAGA TAGCAAACGG 
GACAAAAATT ACTTGCGTGT TGATCAGGGA TCTCGTGCGT CAAAGAGAGT GCATATCAGA 
CGATCTAGTT CAGTAGAGAT TATCAGGCCA AAACGACTCA GTTCCGGAGC AAAAGATATC 
AAAAATCGAG AACGGAGGAA AAGGCTGGGA ACAGATACAA GTAGCGGGTC GAATCGTTCC 
AAGAAAACCA ACTCCTCGTC TTCATCTGTT CAGAAACACT TGCCAAAATA CATTCAATCA 
TTATTCCGAA GTCGGATGGG AACTGATCCA TGCAAGAGGA CATCGAGAAG AGACTCTAAT 
GAGAGTAATA CAAGTGGAAG TGATGCTGTT CTTGAGTTCA CAAGAAAACT TCAAAATTAT 
CCGTTGACGA GACGCGAAGC GCAAAGATTG ACGTATCGAA GCAATGGGCA ACAAGATTCA 
AATGACTCGA AGATGGGACC ACGAACTTTA GGATACGTTT ACCTCCCAGA ACTAGAAATT 
CAAGGTGCAA GTCCGCCAAG ATCACCGGGA CCTCTAACTG GAAACTCGTT AGAAGAAGGA 
ACCACAATGC TTTTGGACAC AAGACGGCCG AGCAGCCCAC AAGCAATCCC CGGCCTTCCT 
TTAATTGAAA TTCGTCGGCC CTCAGCTCTC TCTCAATTTG AATTTGGATA CTTTGTAAAC 
TCACCAGAAT TATCAGGAAG TGAAGTATCT GATTGTGCCC CATTACTTCT CAGTGGTCAA 
ATTGGTGGAA GCCGAAAAAG CTCCGACGAA ACATCATTGG GAGGATGGTC CAGCCGTGCT 
TCATCGGTAA TGAGTCAAAG GTCGTCCAGA TCCAGTGTTG GCTTGAGGTT ATCAACATTT 
TCTGGAGGGA CGAGTATTGC TTCGGATAAC TCTGGGCCAT TTTTGTTCAG TTTTGTTTTG 
AGAAAACGTG CATCTACTAT AGGAACTAGA ATACCAATAC CTAGGAGAGC TTTGTCAAGA 
TCTTCAGGAG ACAGTCTGAG AGTACCTGAT AGAGAAAGTC CACTTCATTT AGCAAGTATG 
ACAGAGATGA ATCCGGATTT TCAATGTGTT CGACAGCTCA TCTTGAACCT TTTGAATGTT 
TACACGAAAA GAAATGACAA TGTGGTTTCT ACAATGAAGG AATGTGCGGA TGTTCTTCGT 
CAAATCCTAA ACTCTCCTCA ACATCCAACT GTCAAGAATT GGTGTGCCGA AATAATTCAT 
GTAGTCAGTA CTCATGTAGA AGAAGAGGAA CCAACGACGG CAGAAAATAA TGAACAAATT 
AATGACGAGT ATCTGGAATT TCAAGACCAA GTGATATCAG GAAGTTTACC ATGCCCGAAG 
GAAGAGGCTG CATACCTTGC AAGTATTCAG TTATCAGTTG AAGAGCAGTG GCCGAGTAAT 
AAAAGGACCC AGACAATTAG AAGACATTTA TTGAAAGGAC AGTTTGGTCG AATCCGGGAT 
CTCGCCCAAA AGATCATGGT GACTCCATGG GAAGTTGATC AAAACTTGTA CTGCACACCA 
CCCAGATTTC CCAATGAATC GGCAAATGCT AGTAGAGCAC AATCAGTGGT TGAGGAAATT 
CAGCACAGAT CAAGAACTCC AACTCTTCTG CGATGTATCA CAAACACAGA CGGCTTAATG 
TCAGAAGAGA TGCAAGCTCA GTGCCTTCCC GTCGATCTAC GTGGTGATCG TAGAACTATC 
AAGCTGGTCA AGGAGAGGAA GCGAAAATTG TTCCATAGTC AAGTCTATGA AAGTGAAATT 
GGCATGAAGA AATTGTACAT TCAGACCGCC AAAAAACTAG CCGCATTTGG TTGTAAGGTT 
TTTCAAGTTA AAGAGTTGCT TCACGGACGA ACACTCAGAA AAACTCTTCG ACTGCTTTGC 
CTTTCTTCTG CTCAATTGTG TCTTCTCGAC GGATCAACAA AACTTGTGCT TAAACGGCAG 
CACGCATCGA CGTTACAACA GTGGAGAGTA GGTGGTGGAG TTAGTAAGCA TCAACTATTG 
CTCGAATTCC GTGGAACCAA GTGGCAACTT ATTGCACCGT CGTATAATGC ACTGAAATCG 
ATATCGATGA CATTATGGGA GATTATGCAA AATTCTGCTA GCAATTCAAT CCAGCGGAGT 
CTCAACCAGT CAATGCATCG TTCGCACACC GAGGCGACTG TCTCAACATC ACGTAGTGAC 
TCAACCTCTT CAACGCAATC AGCCACAGCT GGATTCATTC TCAATGAAGA ACCAATCACA 
CTGTTCCGAC TTGAACTCGA ACGACTTCAA TATATTCTTC ATTTCCCTGA AGAAGTTGCA 
TTCCAACTAT CTTCTACTGA GTATCAGCTT TTCTACTCAA TACAACCCAT GGATTATGTT 
CGTTACGTCA GTTGCGATCT CACATCTGTT CCAGTGTCAG AAAATCCAAG TCCAGTGCGA 
AATCTTGTGA AAAGGCTATC AGAGGTATCA TCCTGGATAA CACATGTTAT TGTCAGTCAG 
CCAACACATG ACGATCGAAA GGTTGCTCTT ACTGCAATTT TGAGAATTGT GGAAACTTGC 
TGGAATATTG GAAACTTTAA TGCAGCTGTT GAGGTTCTTA TGGGTTTAAA GAGTGAAAAA 
CTCAGACCAT TCTGGCTTTC TTTGCGACAA GAGGAAAAGT CTCAGTTTGA TTCGCTTTGC 
GAAACCTTGC TTCCTGCAAA CCAAGCTCTC CCATCTCAAG CTTACATCAA CGCGGTCCAA 
CGAGCACTGA GAATGCCACA ATCTCGTGTG ATTCCATTTT TTGGAATATT TCTTCGTGAT 
CTCTACGCAA TTGTGAATGA CTTGCCGAAT ATCGTTGTGA TTGGACAAGA AGGTGAAACC 
CAAAAGCTGG AATTCATGAG CGATCCCAAT GGCGAAGATC ATTTTTCGTC GAGAATTGGA 
GTGGGTGGGC TGTTGAATGC TGATAAAATC AACTTGGTGG CAATTGTTTT GGACAACTTG 
GAATTATTCC ATCGTCACAG CAGGACAATG ATCAAATTGT TAGAAGAGCA AGCAGTCCCA 
CCAATTCAAA TTCCTCAGAA CGAGCGGGAG CAAAAAGAAA AGGAAGCAAA AACATATGAA 
CCAGTACAAG TGGTTCGCGG CTCTTCTCAT GGAGTGGCTC TCATCCCTCT TGATACCCTA 
ACATTCGATC TTGATGTTAT CCAACGACTC CAGCACGGAA CAACAGTGAT CCATTACGAA 
CCTGATTCGG GAAGAAGCAA TTTGTGTCTG CTGCGGTTAG ACCCTAGTTG CGGTCAAATA 
AACTGGCATA AGATTTCATA TTCAGTTAAC AAAGATCCCA AAGAAAAGGA TGTGCTCGCC 
AAAGTATCCG TTTCAAATCT GCAGCCGCTA GACTCTGGCC GAGGGGCTCC GTCACCAATG 
CCATCTGGAA GAACACCAGG AACTGGAGGA GTTGGAGTAG AGGAGGGAGA ACTCAAATTA 
AGTGTTGTGA AAGGAGTCGA ACTAGTTGAT AGTTATGATA TTGATATTGA GGCAATTTAC 
CGTCGTCATT CTATGGAAGA GATGTCAGTT CCTGTTAGCT GTTGGAAAGT TAGTCATGGT 
CAGTTGTTAT CGGATAATGA GTTCATCTAC TTTTTGGCCC CTCAACAAAT TGCTCAATTT 
TGGACCAACG GGTTGCAATC TGTAGTTAAA AGTCTCCAAG GACAGCAAAG GTACCCTGAT 
CGGCGAATGT TATGGATCAA AAATGTCTAC TTATCACTTT ACGAGATAAC GGGCGAATCA 
AACTGTGGAC CACGGCCATT TGAAGCACTT CAGGCATTTG GGCTGAGTCA AACAAATACT 
AACGCAACAC GACCCAATGA CTCGTCGCTG TCCAGTGAGC CAGGAGGAGC TAAAAGCAGA 
CTGAAGAACC TTAAAAATGC TATGCAAAAG AAGCTTCGTG GTGCATCGCG CGAAGGAAGC 
AGATCTCAGT CACCTCAACC TCATTCTCCT CTTGTCCGAC CACCATCAAT AAAATCGCAA 
ATATCGTCAC AATCTGGTCC GCCGGGTCCG AACTCTCCAG GCTATTTGCT GAAACCACGT 
GGAGAACCTG CCAATAGTGA TGCAGGAGAC ATTGATAGCA TTTATACACC TCGATCAAGA 
ACTCCAACAA GCAGCTCCTA TGGAGGGCGA TCCGTTGGAG GGAGATCATG TAAATCGTGG 
AGATCAAGAG GAGGAGAGAC ACCAAATTCG GGTTCCATAA GCAGCTCGGG TCAGATGTCA 
ATTCAAGTGT CTGGTCTGAG TGGACCATCG GGAAAAGAGT TTCAGGAAAA ACCATTAACA 
CTTGTAGAGT TCGCTGAACT TTTTCGATTG TTCAATACAA GAATGAGAAA AGATTTGAGA 
GATGTGTTCA ATGATGTCCT GTCTACGGCA ACTACTCCTC AGCATTGTCC TAAAAGAGAA 
CGTGATCGCC ACAGTCCGAG AATGCAGTCA AGGCTGGCGA GTGTCTCCAA CTCGTACAAT 
GCAGATTTTC TGTCTAACGA TTTCTTGACT CGGAATACGG CAGTAACGTC CCATCATATC 
AGTGAAAAGC AAAACAAAAT CTACAATGCA TTGGCACTTG CGAGTGTTAA TAGCATGGGC 
GGACTCATGG ACACTTCCCG GTCGTCGATG CTGACGCCGC AAATGCTGCG CGCTTTTGTC 
AACACTCATC AGATGGAGCA GATTGACGAG CAAACGGCGA TTAAGCTTAT TCAGGATCAT 
GAACCTGATG GTATCTGTCG ACAAAAGAAT CAAATGTCAT TCGAAGGATT CACTAGATTC 
CTTTGCGATC CTGTCAACTT TGCATTCGTA CCGGAAACAA TTGAACCAGA TGAAGAGGAC 
CTACGGTACC CGTTGTCTCA TTATTACATC AATTCAAGTC ACAACACCTA TTTGACGGGA 
CATCAACTCA AGGGACCCAG TAGCTCGGAG ATGTACAGAC AGGTTCTCCT CACCGGGTGT 
CGGTGTGTCG AGCTTGATTG TTGGGATGGA GATGACGGAT TGCCATTGAT TTATCATGGA 
CACACATTGG TTAGCAAGAT TGGGTTTAGA CAGGTGGTCG AAATTATCAA AAAATCTGCA 
TTCATAACAT CGGATCTTCC TGTGATATTA TCAATTGAGA ATCATTGTTC CCTACAACAA 
CAGGCGAAAA TGGCACAGAT GTTCAAAACA GTTCTTGGAG ATTTACTAGT ATCAAACTTC 
CTTTTTGAAG CTGATTTCTC GGACTCTCCA CGTCTTCCAT GCCCTCTCCA AATGAAAAAT 
AAGATCTTGA TAAAAAATAA GAAAATGATA GTCGACCCAC CAACTCCATT GCCAATGATT 
GAACGAGGAG CTGTGCAGCG TGGTGAAACT CAATTAAATC TTCACAGAAA ACAGAGTAAA 
AACTCCTACG AAAGCAGTAC GGTAGATGAA GTGGAAGATG ACGACCTAGA TGAGTTTCTT 
GACGATGAAG AAAATGAGGA GGATGACCAA GAAGAGGTTC AAGTCAGAAG TGAAAAAGAA 
GACAGTCCGA AGACATCAAA ACGTGCAGAG AAATCGGCTA GAAACATCAA ACAGCAAGAT 
TCGTTATGTA GCGATCATTC GGTAGAGCAG GCAAAACCTT CCACAAGCAA GACCACATCC 
AAAACCAACG ATAGAAAGAC TGAAGATGAA GTACTTTACG CTCAACTTGC TCAAAATGCC 
ATCCGAAACC AACAACCAAG AAAGAACAAT ACTGGAGTCC AAATAGCGCC AGAGTTGTCA 
GATATTGTCA TTTACATGCA AGCGACAAAA TTCAAAGGAT TTCCCCCAGT CGATGGCATT 
CAATCACCAA GGATAATGGA GGAAGGGCCA GCTTCTGCAA GTCTTTCATT TTCATCACGG 
GCTCGTACGC CATCAAATCT GCTGAATACA CCAGCCCCTC CACGACGACA GCGAAGTAGC 
ACTCAGTTGA GTCAAGAACT GGCTGCAGAA TTTTTGGGAT CAGTTAGGGC AAATGCTACA 
GCTACATGCT ACCAGGTTAC ATCTCTTAAT GAAAACGCTG CAAAGAAACT GATGAAAAGA 
CATCCAGCAA AATGCGTATC TTATACTCGG GACCATCTGA TTCGTACGTA TCCAAGTGCC 
AAGCACTATG ACTCGTCCAA CTTTAACCCC ATCAACTGCT GGGCTCATGG AATGCAAATG 
GTTGCTTTAA ATTTTCAAAC TCCTGATGTT ATAATGGCAG TTAACCAAGC GATGTTCGAG 
CAATCTGGAA ACTGTGGATA TCAACTGAAA CCACGTTGTC TTTGGGATGA ATCTCATCTT 
TTGTACAACA AATTTTTGCC TCTCTCCAAA GACATTGCCG GCCATTCTGC TTTGTTACTA 
AACTTAACTA TTATATCCGG ACAGCACGTG TATCCAAACA CCCATTACGC CAGTTTATAT 
GTCGAAATTG AAGTCATCGG TATTCACAAC GACTGTGTTC GTGAGAAATC AAAAGTGGTC 
CAGAGAAATT CTGTGAATCC AATCTGGAAC CACACCACTC AACTTCGAAT AGCTTGTGTT 
GACTTAGCAT TCCTTCGCAT AGCAGTATGT GATAGTGGGC AAAATGGAAG AGTCGTTGCT 
CACCGAGTTG TTCCTGTCAA ATGCATTCGT CCGGGTTTCC GACATCTCCC ACTCAGAACT 
CCAACTAATC TTCCGATCGA CAACGCTATG ATATTTTTAA GAACAAGATT CGAGCAAGAA 
GAACACATCT ACCTCCACGA CGATGATTCA AACACCTATT GTAATCTAGA ACATACGCTG 
GCATACCGCA CAGACTTAAC ACCCAATTTA TCTCCAACGC CAATTTTGAA GAAACAGATC 
TTTGTTCTTC GAATTACAGG CGCGTTTGCT GATGAAACAG CAATTACAGT GCACAGCGAG 
TCAGGAAGTA CAGTGAAGAC GGTGATGCAA CAGGCGCTGC TTAACGCTGG AAAGAATGCG 
GATCAGGTTG AAGAGTATGT ACTCATCGAA GAATCACTGC CGGCACCTTC TGGGGAAGAT 
CCAATTGAGC AGAGAGTACT TCCTCTGAAC GAGCCTATCA TGGACGCCGT GGCCTGCTGG 
AACGGTTCAA TGCGTCGTTT TGTGTTACGA AAAAAAGGCA GTGACCCATC ATCTAGAGCT 
TGGATCACTT CAATTATAAA ATCCGGAACA AGTGGAAGCT CCACGTCAGT CTCACCAAGT 
CCGTTGACCA AAGATGGACA CGTAAAGAGT GCATCAAGCA ATCAACTTCA TGGACGATCA 
TTGGACACTG ACGCTTTTGG AGAGCACCTT GAAGTGACAG AAGGGTTGAA CCCTCGTGCA 
AGATCAATGG GCGACACATT TCTGGTATGT GTTCACAATG TTAGTGAAGA TCAGCCTTAT 
GCAATCCTAC GTGCTGGAAT CCACAGCACT GCCGCCGACA TTATTCGTCA GGTGTTTGTT 
AAAGCTCGCC GTTCAAATGT AGACGATTCC GAGTTTGTTC TTGTTGAAGA AACCTGCGAT 
GATCCAAAAC TCAATCAAGG CCAAATGACT CCAAAGTATC CAAACAACCG CACCACAAGC 
CGAGTGTTAG GACAAAACGA AAATGTTTGG AAAGCACAAT CGAGGTGGAA GTCAATGGGC 
AGATTTGTAC TTGAAAACCG AAAGGACACA GTGCACGCAA CCCTCGAAAA AGTGAGATCA 
TTTATATCAA AACTGGAAGC TGCAAAAGCA AGCATGGATC CCAGACACGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001360639.1
CDS	1..10494
Translation	MSLRQASHKFPSVDSMPTSRDSSMETTDRYPFGYTTELARTEPSASTAKRLRQRLQRQQSGQSGLSGRRYTGESSECSSLVGTPTDTGRSFTFGAAQRVMRERDDRKPSVTQNLSEIMLEAIHYEDVNLVERLLTAHSSKQPMSTSPSIGSTNTLTDLAQRRHGAGAHRRSNASSTVSTHSGGRSTCAMMNTLHMAVAHKQRDIVELLLKNGYDPNTPASCHCKGNCTATGNIPLTSIIPRTHSMTPELCSTCSQLRVVSIVDQTPLGVAVRSQSSELIALLIAYGGDVNLGDEDGNTPLMLAVRESPLSWPCLHTLIFFGAQIEQKNMRGICPLDLAPELKKLQQTCVEELFKNACSGDCEPPSRSTPGPSAKNWNRLQVDNDPVIDCVSWEQAWELLKKMACNPECLDNIVNSLSKFATQMENTPSSVDRDQFDAHMGGLLHKMIHTAIEEYESSTPTYKKQKKLHLTWLLSQIATFCFTFLQKSGTSRQFSALNTLNKIIDAGLVHDLFAFNDVVFHSSRILNRCHAFDSDEPPTPSGESFNSNADQNSTVDHVFIYGAGYKDQSLLPSPTVAPTRHKSDLMNSFSEMDPSQVIVALHNAITMQNREVGSRSVCSPAHRWRQCCAHCTQILVARLLLFLTHAKKFRSRLSERHQLRSLVALLEPTLEPQLLCLLLQVLALIALDPSTHTTLIDIQIDDVLIQMLLPADDWYYTNHSTKYGTFVKYHAARVLVYVGMGDRVGSRVNLFNMMTFEPSAKSGSQQNEDDYICETCATPRSRSTFSRSAVSVEGVLLKVLAEVAELVRQSQHLSASEPITEESPSAGSPPAVLPNQAEQKSINEKNAEKLTQLGCQILISLEQLEAHLCKLGLVLDSVLLLRLLLHKLSWDLGLVTKKRVAVVDHVYKPITDPRAHSSCSLGNNRNFGKSKSFDRREDSKRDKNYLRVDQGSRASKRVHIRRSSSVEIIRPKRLSSGAKDIKNRERRKRLGTDTSSGSNRSKKTNSSSSSVQKHLPKYIQSLFRSRMGTDPCKRTSRRDSNESNTSGSDAVLEFTRKLQNYPLTRREAQRLTYRSNGQQDSNDSKMGPRTLGYVYLPELEIQGASPPRSPGPLTGNSLEEGTTMLLDTRRPSSPQAIPGLPLIEIRRPSALSQFEFGYFVNSPELSGSEVSDCAPLLLSGQIGGSRKSSDETSLGGWSSRASSVMSQRSSRSSVGLRLSTFSGGTSIASDNSGPFLFSFVLRKRASTIGTRIPIPRRALSRSSGDSLRVPDRESPLHLASMTEMNPDFQCVRQLILNLLNVYTKRNDNVVSTMKECADVLRQILNSPQHPTVKNWCAEIIHVVSTHVEEEEPTTAENNEQINDEYLEFQDQVISGSLPCPKEEAAYLASIQLSVEEQWPSNKRTQTIRRHLLKGQFGRIRDLAQKIMVTPWEVDQNLYCTPPRFPNESANASRAQSVVEEIQHRSRTPTLLRCITNTDGLMSEEMQAQCLPVDLRGDRRTIKLVKERKRKLFHSQVYESEIGMKKLYIQTAKKLAAFGCKVFQVKELLHGRTLRKTLRLLCLSSAQLCLLDGSTKLVLKRQHASTLQQWRVGGGVSKHQLLLEFRGTKWQLIAPSYNALKSISMTLWEIMQNSASNSIQRSLNQSMHRSHTEATVSTSRSDSTSSTQSATAGFILNEEPITLFRLELERLQYILHFPEEVAFQLSSTEYQLFYSIQPMDYVRYVSCDLTSVPVSENPSPVRNLVKRLSEVSSWITHVIVSQPTHDDRKVALTAILRIVETCWNIGNFNAAVEVLMGLKSEKLRPFWLSLRQEEKSQFDSLCETLLPANQALPSQAYINAVQRALRMPQSRVIPFFGIFLRDLYAIVNDLPNIVVIGQEGETQKLEFMSDPNGEDHFSSRIGVGGLLNADKINLVAIVLDNLELFHRHSRTMIKLLEEQAVPPIQIPQNEREQKEKEAKTYEPVQVVRGSSHGVALIPLDTLTFDLDVIQRLQHGTTVIHYEPDSGRSNLCLLRLDPSCGQINWHKISYSVNKDPKEKDVLAKVSVSNLQPLDSGRGAPSPMPSGRTPGTGGVGVEEGELKLSVVKGVELVDSYDIDIEAIYRRHSMEEMSVPVSCWKVSHGQLLSDNEFIYFLAPQQIAQFWTNGLQSVVKSLQGQQRYPDRRMLWIKNVYLSLYEITGESNCGPRPFEALQAFGLSQTNTNATRPNDSSLSSEPGGAKSRLKNLKNAMQKKLRGASREGSRSQSPQPHSPLVRPPSIKSQISSQSGPPGPNSPGYLLKPRGEPANSDAGDIDSIYTPRSRTPTSSSYGGRSVGGRSCKSWRSRGGETPNSGSISSSGQMSIQVSGLSGPSGKEFQEKPLTLVEFAELFRLFNTRMRKDLRDVFNDVLSTATTPQHCPKRERDRHSPRMQSRLASVSNSYNADFLSNDFLTRNTAVTSHHISEKQNKIYNALALASVNSMGGLMDTSRSSMLTPQMLRAFVNTHQMEQIDEQTAIKLIQDHEPDGICRQKNQMSFEGFTRFLCDPVNFAFVPETIEPDEEDLRYPLSHYYINSSHNTYLTGHQLKGPSSSEMYRQVLLTGCRCVELDCWDGDDGLPLIYHGHTLVSKIGFRQVVEIIKKSAFITSDLPVILSIENHCSLQQQAKMAQMFKTVLGDLLVSNFLFEADFSDSPRLPCPLQMKNKILIKNKKMIVDPPTPLPMIERGAVQRGETQLNLHRKQSKNSYESSTVDEVEDDDLDEFLDDEENEEDDQEEVQVRSEKEDSPKTSKRAEKSARNIKQQDSLCSDHSVEQAKPSTSKTTSKTNDRKTEDEVLYAQLAQNAIRNQQPRKNNTGVQIAPELSDIVIYMQATKFKGFPPVDGIQSPRIMEEGPASASLSFSSRARTPSNLLNTPAPPRRQRSSTQLSQELAAEFLGSVRANATATCYQVTSLNENAAKKLMKRHPAKCVSYTRDHLIRTYPSAKHYDSSNFNPINCWAHGMQMVALNFQTPDVIMAVNQAMFEQSGNCGYQLKPRCLWDESHLLYNKFLPLSKDIAGHSALLLNLTIISGQHVYPNTHYASLYVEIEVIGIHNDCVREKSKVVQRNSVNPIWNHTTQLRIACVDLAFLRIAVCDSGQNGRVVAHRVVPVKCIRPGFRHLPLRTPTNLPIDNAMIFLRTRFEQEEHIYLHDDDSNTYCNLEHTLAYRTDLTPNLSPTPILKKQIFVLRITGAFADETAITVHSESGSTVKTVMQQALLNAGKNADQVEEYVLIEESLPAPSGEDPIEQRVLPLNEPIMDAVACWNGSMRRFVLRKKGSDPSSRAWITSIIKSGTSGSSTSVSPSPLTKDGHVKSASSNQLHGRSLDTDAFGEHLEVTEGLNPRARSMGDTFLVCVHNVSEDQPYAILRAGIHSTAADIIRQVFVKARRSNVDDSEFVLVEETCDDPKLNQGQMTPKYPNNRTTSRVLGQNENVWKAQSRWKSMGRFVLENRKDTVHATLEKVRSFISKLEAAKASMDPRHE

Target ORF information:

RefSeq Version	NM_001373491.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans PhosphoLipase C (plc-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001373491.1

ORF Insert Sequence:

ATGTCCTTAC GCCAAGCATC ACACAAATTT CCATCGGTGG ACTCAATGCC AACATCACGG 
GATTCATCGA TGGAAACAAC CGACCGGTAC CCGTTTGGGT ATACGACCGA ATTGGCACGT 
ACCGAACCAT CTGCATCAAC TGCCAAACGT CTTCGACAAA GGTTACAGCG ACAGCAAAGT 
GGTCAATCAG GATTATCCGG TCGACGGTAT ACTGGAGAAA GCAGCGAGTG CTCATCACTT 
GTCGGAACAC CCACAGACAC TGGCAGATCA TTTACATTTG GAGCAGCACA AAGAGTCATG 
CGGGAACGGG ATGACCGCAA ACCATCAGTT ACCCAGAACT TGTCAGAAAT AATGCTCGAG 
GCAATACATT ATGAAGATGT GAACTTGGTG GAAAGGTTGC TCACTGCGCA TAGTTCAAAA 
CAACCAATGT CAACATCTCC GTCAATTGGT AGCACAAACA CATTGACGGA TCTTGCTCAA 
CGTAGACACG GTGCGGGGGC ACACCGACGT TCCAATGCCA GTAGTACGGT GTCCACACAT 
TCCGGAGGAA GATCCACGTG TGCTATGATG AACACGTTGC ACATGGCTGT GGCTCATAAA 
CAACGAGATA TAGTGGAACT TTTGCTTAAA AACGGCTACG ATCCCAATAC TCCCGCATCG 
TGTCACTGTA AGGGAAATTG CACGGCGACT GGGAATATTC CACTGACTTC GATCATTCCG 
AGAACTCATT CAATGACGCC AGAATTGTGT TCGACGTGCT CTCAACTGAG GGTTGTGTCA 
ATCGTGGATC AAACACCGCT TGGTGTGGCA GTCAGATCAC AGTCCAGCGA ATTGATAGCA 
TTGTTGATTG CCTACGGAGG AGATGTTAAT TTAGGTGATG AAGATGGCAA CACGCCGTTG 
ATGCTAGCCG TACGAGAATC ACCTCTGTCT TGGCCTTGCC TGCACACGCT CATTTTCTTT 
GGAGCTCAAA TTGAACAAAA GAACATGCGC GGAATATGCC CTCTTGATCT TGCTCCAGAG 
CTGAAGAAAC TTCAACAAAC TTGCGTTGAA GAGCTTTTCA AAAATGCGTG CTCGGGTGAT 
TGTGAACCAC CATCTCGATC TACACCGGGA CCATCAGCCA AAAACTGGAA TAGACTACAA 
GTTGACAATG ATCCTGTAAT TGACTGCGTC AGTTGGGAAC AAGCATGGGA ATTGCTGAAG 
AAGATGGCAT GTAATCCAGA ATGTCTAGAT AACATCGTGA ATTCATTGTC AAAATTTGCA 
ACCCAAATGG AGAACACCCC GTCAAGCGTT GATCGTGATC AATTCGATGC ACACATGGGT 
GGCCTACTAC ATAAAATGAT TCATACAGCG ATTGAGGAGT ACGAGTCTTC GACACCAACC 
TACAAAAAGC AGAAGAAACT CCATCTCACC TGGCTGCTCA GCCAAATTGC CACGTTTTGT 
TTTACATTTC TGCAGAAAAG TGGAACGAGC CGACAATTCT CAGCACTGAA CACGTTGAAT 
AAAATTATTG ATGCTGGTTT AGTACATGAT TTGTTCGCAT TCAACGACGT TGTCTTTCAT 
TCTTCAAGAA TTTTGAACCG ATGCCATGCA TTTGATTCCG ACGAACCACC AACTCCATCT 
GGCGAATCTT TCAACTCAAA TGCAGACCAA AACTCAACTG TCGATCATGT TTTCATTTAC 
GGAGCTGGCT ATAAAGACCA ATCGTTATTA CCTTCTCCTA CGGTGGCACC AACTCGACAC 
AAGTCCGACT TAATGAATTC TTTCAGCGAA ATGGATCCAA GCCAGGTCAT TGTGGCCTTG 
CACAACGCGA TAACAATGCA AAACCGGGAA GTAGGGTCAA GATCTGTTTG TAGTCCGGCT 
CATCGATGGA GGCAGTGTTG TGCTCATTGT ACACAGATAT TGGTGGCCAG ATTATTGCTA 
TTCTTAACAC ACGCAAAAAA ATTTCGAAGT CGGTTGTCTG AACGACATCA GCTCAGAAGC 
TTGGTAGCTT TGTTAGAACC AACTTTGGAA CCTCAACTCT TATGTCTTTT ACTTCAAGTT 
CTAGCTCTTA TAGCGTTAGA TCCATCTACC CACACAACTT TGATAGACAT TCAAATTGAC 
GACGTCTTGA TTCAAATGCT ACTCCCAGCA GATGATTGGT ACTACACTAA TCACAGCACT 
AAATATGGAA CTTTTGTAAA GTACCATGCT GCTAGAGTTT TAGTGTACGT TGGAATGGGT 
GATCGAGTAG GAAGTCGAGT CAATCTGTTC AACATGATGA CGTTTGAGCC ATCTGCAAAA 
AGCGGGAGTC AACAAAACGA GGACGACTAC ATTTGTGAGA CATGTGCCAC ACCGCGCAGC 
CGAAGTACAT TTTCAAGATC AGCAGTTTCA GTGGAAGGTG TTCTGTTAAA AGTGTTGGCA 
GAAGTTGCTG AGCTTGTACG TCAGAGTCAA CACCTATCAG CAAGTGAACC AATAACTGAA 
GAATCGCCCA GTGCAGGATC TCCACCCGCT GTCCTACCAA ATCAGGCTGA ACAGAAAAGT 
ATTAACGAAA AAAATGCAGA GAAACTTACA CAACTTGGGT GCCAAATTTT GATCTCATTG 
GAACAACTTG AAGCTCATTT ATGCAAACTT GGACTGGTAC TTGATTCGGT TCTACTTCTC 
CGTCTACTTC TTCATAAGTT GTCATGGGAT CTTGGATTAG TCACAAAAAA GAGGGTTGCT 
GTGGTGGATC ATGTTTACAA ACCAATCACG GATCCGAGAG CTCATTCTAG CTGCAGTCTT 
GGAAACAACA GAAACTTTGG TAAATCTAAA TCTTTTGATA GGCGAGAAGA TAGCAAACGG 
GACAAAAATT ACTTGCGTGT TGATCAGGGA TCTCGTGCGT CAAAGAGAGT GCATATCAGA 
CGATCTAGTT CAGTAGAGAT TATCAGGCCA AAACGACTCA GTTCCGGAGC AAAAGATATC 
AAAAATCGAG AACGGAGGAA AAGGCTGGGA ACAGATACAA GTAGCGGGTC GAATCGTTCC 
AAGAAAACCA ACTCCTCGTC TTCATCTGTT CAGAAACACT TGCCAAAATA CATTCAATCA 
TTATTCCGAA GTCGGATGGG AACTGATCCA TGCAAGAGGA CATCGAGAAG AGACTCTAAT 
GAGAGTAATA CAAGTGGAAG TGATGCTGTT CTTGAGTTCA CAAGAAAACT TCAAAATTAT 
CCGTTGACGA GACGCGAAGC GCAAAGATTG ACGTATCGAA GCAATGGGCA ACAAGATTCA 
AATGACTCGA AGATGGGACC ACGAACTTTA GGATACGTTT ACCTCCCAGA ACTAGAAATT 
CAAGGTGCAA GTCCGCCAAG ATCACCGGGA CCTCTAACTG GAAACTCGTT AGAAGAAGGA 
ACCACAATGC TTTTGGACAC AAGACGGCCG AGCAGCCCAC AAGCAATCCC CGGCCTTCCT 
TTAATTGAAA TTCGTCGGCC CTCAGCTCTC TCTCAATTTG AATTTGGATA CTTTGTAAAC 
TCACCAGAAT TATCAGGAAG TGAAGTATCT GATTGTGCCC CATTACTTCT CAGTGGTCAA 
ATTGGTGGAA GCCGAAAAAG CTCCGACGAA ACATCATTGG GAGGATGGTC CAGCCGTGCT 
TCATCGGTAA TGAGTCAAAG GTCGTCCAGA TCCAGTGTTG GCTTGAGGTT ATCAACATTT 
TCTGGAGGGA CGAGTATTGC TTCGGATAAC TCTGGGCCAT TTTTGTTCAG TTTTGTTTTG 
AGAAAACGTG CATCTACTAT AGGAACTAGA ATACCAATAC CTAGGAGAGC TTTGTCAAGA 
TCTTCAGGAG ACAGTCTGAG AGTACCTGAT AGAGAAAGTC CACTTCATTT AGCAAGTATG 
ACAGAGATGA ATCCGGATTT TCAATGTGTT CGACAGCTCA TCTTGAACCT TTTGAATGTT 
TACACGAAAA GAAATGACAA TGTGGTTTCT ACAATGAAGG AATGTGCGGA TGTTCTTCGT 
CAAATCCTAA ACTCTCCTCA ACATCCAACT GTCAAGAATT GGTGTGCCGA AATAATTCAT 
GTAGTCAGTA CTCATGTAGA AGAAGAGGAA CCAACGACGG CAGAAAATAA TGAACAAATT 
AATGACGAGT ATCTGGAATT TCAAGACCAA GTGATATCAG GAAGTTTACC ATGCCCGAAG 
GAAGAGGCTG CATACCTTGC AAGTATTCAG TTATCAGTTG AAGAGCAGTG GCCGAGTAAT 
AAAAGGACCC AGACAATTAG AAGACATTTA TTGAAAGGAC AGTTTGGTCG AATCCGGGAT 
CTCGCCCAAA AGATCATGGT GACTCCATGG GAAGTTGATC AAAACTTGTA CTGCACACCA 
CCCAGATTTC CCAATGAATC GGCAAATGCT AGTAGAGCAC AATCAGTGGT TGAGGAAATT 
CAGCACAGAT CAAGAACTCC AACTCTTCTG CGATGTATCA CAAACACAGA CGGCTTAATG 
TCAGAAGAGA TGCAAGCTCA GTGCCTTCCC GTCGATCTAC GTGGTGATCG TAGAACTATC 
AAGCTGGTCA AGGAGAGGAA GCGAAAATTG TTCCATAGTC AAGTCTATGA AAGTGAAATT 
GGCATGAAGA AATTGTACAT TCAGACCGCC AAAAAACTAG CCGCATTTGG TTGTAAGGTT 
TTTCAAGTTA AAGAGTTGCT TCACGGACGA ACACTCAGAA AAACTCTTCG ACTGCTTTGC 
CTTTCTTCTG CTCAATTGTG TCTTCTCGAC GGATCAACAA AACTTGTGCT TAAACGGCAG 
CACGCATCGA CGTTACAACA GTGGAGAGTA GGTGGTGGAG TTAGTAAGCA TCAACTATTG 
CTCGAATTCC GTGGAACCAA GTGGCAACTT ATTGCACCGT CGTATAATGC ACTGAAATCG 
ATATCGATGA CATTATGGGA GATTATGCAA AATTCTGCTA GCAATTCAAT CCAGCGGAGT 
CTCAACCAGT CAATGCATCG TTCGCACACC GAGGCGACTG TCTCAACATC ACGTAGTGAC 
TCAACCTCTT CAACGCAATC AGCCACAGCT GGATTCATTC TCAATGAAGA ACCAATCACA 
CTGTTCCGAC TTGAACTCGA ACGACTTCAA TATATTCTTC ATTTCCCTGA AGAAGTTGCA 
TTCCAACTAT CTTCTACTGA GTATCAGCTT TTCTACTCAA TACAACCCAT GGATTATGTT 
CGTTACGTCA GTTGCGATCT CACATCTGTT CCAGTGTCAG AAAATCCAAG TCCAGTGCGA 
AATCTTGTGA AAAGGCTATC AGAGGTATCA TCCTGGATAA CACATGTTAT TGTCAGTCAG 
CCAACACATG ACGATCGAAA GGTTGCTCTT ACTGCAATTT TGAGAATTGT GGAAACTTGC 
TGGAATATTG GAAACTTTAA TGCAGCTGTT GAGGTTCTTA TGGGTTTAAA GAGTGAAAAA 
CTCAGACCAT TCTGGCTTTC TTTGCGACAA GAGGAAAAGT CTCAGTTTGA TTCGCTTTGC 
GAAACCTTGC TTCCTGCAAA CCAAGCTCTC CCATCTCAAG CTTACATCAA CGCGGTCCAA 
CGAGCACTGA GAATGCCACA ATCTCGTGTG ATTCCATTTT TTGGAATATT TCTTCGTGAT 
CTCTACGCAA TTGTGAATGA CTTGCCGAAT ATCGTTGTGA TTGGACAAGA AGGTGAAACC 
CAAAAGCTGG AATTCATGAG CGATCCCAAT GGCGAAGATC ATTTTTCGTC GAGAATTGGA 
GTGGGTGGGC TGTTGAATGC TGATAAAATC AACTTGGTGG CAATTGTTTT GGACAACTTG 
GAATTATTCC ATCGTCACAG CAGGACAATG ATCAAATTGT TAGAAGAGCA AGCAGTCCCA 
CCAATTCAAA TTCCTCAGAA CGAGCGGGAG CAAAAAGAAA AGGAAGCAAA AACATATGAA 
CCAGTACAAG TGGTTCGCGG CTCTTCTCAT GGAGTGGCTC TCATCCCTCT TGATACCCTA 
ACATTCGATC TTGATGTTAT CCAACGACTC CAGCACGGAA CAACAGTGAT CCATTACGAA 
CCTGATTCGG GAAGAAGCAA TTTGTGTCTG CTGCGGTTAG ACCCTAGTTG CGGTCAAATA 
AACTGGCATA AGATTTCATA TTCAGTTAAC AAAGATCCCA AAGAAAAGGA TGTGCTCGCC 
AAAGTATCCG TTTCAAATCT GCAGCCGCTA GACTCTGGCC GAGGGGCTCC GTCACCAATG 
CCATCTGGAA GAACACCAGG AACTGGAGGA GTTGGAGTAG AGGAGGGAGA ACTCAAATTA 
AGTGTTGTGA AAGGAGTCGA ACTAGTTGAT AGTTATGATA TTGATATTGA GGCAATTTAC 
CGTCGTCATT CTATGGAAGA GATGTCAGTT CCTGTTAGCT GTTGGAAAGT TAGTCATGGT 
CAGTTGTTAT CGGATAATGA GTTCATCTAC TTTTTGGCCC CTCAACAAAT TGCTCAATTT 
TGGACCAACG GGTTGCAATC TGTAGTTAAA AGTCTCCAAG GACAGCAAAG GTACCCTGAT 
CGGCGAATGT TATGGATCAA AAATGTCTAC TTATCACTTT ACGAGATAAC GGGCGAATCA 
AACTGTGGAC CACGGCCATT TGAAGCACTT CAGGCATTTG GGCTGAGTCA AACAAATACT 
AACGCAACAC GACCCAATGA CTCGTCGCTG TCCAGTGAGC CAGGAGGAGC TAAAAGCAGA 
CTGAAGAACC TTAAAAATGC TATGCAAAAG AAGCTTCGTG GTGCATCGCG CGAAGGAAGC 
AGATCTCAGT CACCTCAACC TCATTCTCCT CTTGTCCGAC CACCATCAAT AAAATCGCAA 
ATATCGTCAC AATCTGGTCC GCCGGGTCCG AACTCTCCAG GCTATTTGCT GAAACCACGT 
GGAGAACCTG CCAATAGTGA TGCAGGAGAC ATTGATAGCA TTTATACACC TCGATCAAGA 
ACTCCAACAA GCAGCTCCTA TGGAGGGCGA TCCGTTGGAG GGAGATCATG TAAATCGTGG 
AGATCAAGAG GAGGAGAGAC ACCAAATTCG GGTTCCATAA GCAGCTCGGG TCAGATGTCA 
ATTCAAGTGT CTGGTCTGAG TGGACCATCG GGAAAAGAGT TTCAGGAAAA ACCATTAACA 
CTTGTAGAGT TCGCTGAACT TTTTCGATTG TTCAATACAA GAATGAGAAA AGATTTGAGA 
GATGTGTTCA ATGATGTCCT GTCTACGGCA ACTACTCCTC AGCATTGTCC TAAAAGAGAA 
CGTGATCGCC ACAGTCCGAG AATGCAGTCA AGGCTGGCGA GTGTCTCCAA CTCGTACAAT 
GCAGATTTTC TGTCTAACGA TTTCTTGACT CGGAATACGG CAGTAACGTC CCATCATATC 
AGTGAAAAGC AAAACAAAAT CTACAATGCA TTGGCACTTG CGAGTGTTAA TAGCATGGGC 
GGACTCATGG ACACTTCCCG GTCGTCGATG CTGACGCCGC AAATGCTGCG CGCTTTTGTC 
AACACTCATC AGATGGAGCA GATTGACGAG CAAACGGCGA TTAAGCTTAT TCAGGATCAT 
GAACCTGATG GTATCTGTCG ACAAAAGAAT CAAATGTCAT TCGAAGGATT CACTAGATTC 
CTTTGCGATC CTGTCAACTT TGCATTCGTA CCGGAAACAA TTGAACCAGA TGAAGAGGAC 
CTACGGTACC CGTTGTCTCA TTATTACATC AATTCAAGTC ACAACACCTA TTTGACGGGA 
CATCAACTCA AGGGACCCAG TAGCTCGGAG ATGTACAGAC AGGTTCTCCT CACCGGGTGT 
CGGTGTGTCG AGCTTGATTG TTGGGATGGA GATGACGGAT TGCCATTGAT TTATCATGGA 
CACACATTGG TTAGCAAGAT TGGGTTTAGA CAGGTGGTCG AAATTATCAA AAAATCTGCA 
TTCATAACAT CGGATCTTCC TGTGATATTA TCAATTGAGA ATCATTGTTC CCTACAACAA 
CAGGCGAAAA TGGCACAGAT GTTCAAAACA GTTCTTGGAG ATTTACTAGT ATCAAACTTC 
CTTTTTGAAG CTGATTTCTC GGACTCTCCA CGTCTTCCAT GCCCTCTCCA AATGAAAAAT 
AAGATCTTGA TAAAAAATAA GAAAATGATA GTCGACCCAC CAACTCCATT GCCAATGATT 
GAACGAGGAG CTGTGCAGCG TGGTGAAACT CAATTAAATC TTCACAGAAA ACAGAGTAAA 
AACTCCTACG AAAGCAGTAC GGTAGATGAA GTGGAAGATG ACGACCTAGA TGAGTTTCTT 
GACGATGAAG AAAATGAGGA GGATGACCAA GAAGAGGTTC AAGTCAGAAG TGAAAAAGAA 
GACAGTCCGA AGACATCAAA ACGTGCAGAG AAATCGGCTA GAAACATCAA ACAGCAAGAT 
TCGTTATGTA GCGATCATTC GGTAGAGCAG GCAAAACCTT CCACAAGCAA GACCACATCC 
AAAACCAACG ATAGAAAGAC TGAAGATGAA GTACTTTACG CTCAACTTGC TCAAAATGCC 
ATCCGAAACC AACAACCAAG AAAGAACAAT ACTGGAGTCC AAATAGCGCC AGAGTTGTCA 
GATATTGTCA TTTACATGCA AGCGACAAAA TTCAAAGGAT TTCCCCCAGT CGATGGCATT 
CAATCACCAA GGATAATGGA GGAAGGGCCA GCTTCTGCAA GTCTTTCATT TTCATCACGG 
GCTCGTACGC CATCAAATCT GCTGAATACA CCAGCCCCTC CACGACGACA GCGAAGTAGC 
ACTCAGTTGA GTCAAGAACT GGCTGCAGAA TTTTTGGGAT CAGTTAGGGC AAATGCTACA 
GCTACATGCT ACCAGGTTAC ATCTCTTAAT GAAAACGCTG CAAAGAAACT GATGAAAAGA 
CATCCAGCAA AATGCGTATC TTATACTCGG GACCATCTGA TTCGTACGTA TCCAAGTGCC 
AAGCACTATG ACTCGTCCAA CTTTAACCCC ATCAACTGCT GGGCTCATGG AATGCAAATG 
GTTGCTTTAA ATTTTCAAAC TCCTGATGTT ATAATGGCAG TTAACCAAGC GATGTTCGAG 
CAATCTGGAA ACTGTGGATA TCAACTGAAA CCACGTTGTC TTTGGGATGA ATCTCATCTT 
TTGTACAACA AATTTTTGCC TCTCTCCAAA GACATTGCCG GCCATTCTGC TTTGTTACTA 
AACTTAACTA TTATATCCGG ACAGCACGTG TATCCAAACA CCCATTACGC CAGTTTATAT 
GTCGAAATTG AAGTCATCGG TATTCACAAC GACTGTGTTC GTGAGAAATC AAAAGTGGTC 
CAGAGAAATT CTGTGAATCC AATCTGGAAC CACACCACTC AACTTCGAAT AGCTTGTGTT 
GACTTAGCAT TCCTTCGCAT AGCAGTATGT GATAGTGGGC AAAATGGAAG AGTCGTTGCT 
CACCGAGTTG TTCCTGTCAA ATGCATTCGT CCGGGTTTCC GACATCTCCC ACTCAGAACT 
CCAACTAATC TTCCGATCGA CAACGCTATG ATATTTTTAA GAACAAGATT CGAGCAAGAA 
GAACACATCT ACCTCCACGA CGATGATTCA AACACCTATT GTAATCTAGA ACATACGCTG 
GCATACCGCA CAGACTTAAC ACCCAATTTA TCTCCAACGC CAATTTTGAA GAAACAGATC 
TTTGTTCTTC GAATTACAGG CGCGTTTGCT GATGAAACAG CAATTACAGT GCACAGCGAG 
TCAGGAAGTA CAGTGAAGAC GGTGATGCAA CAGGCGCTGC TTAACGCTGG AAAGAATGCG 
GATCAGGTTG AAGAGTATGT ACTCATCGAA GAATCACTGC CGGCACCTTC TGGGGAAGAT 
CCAATTGAGC AGAGAGTACT TCCTCTGAAC GAGCCTATCA TGGACGCCGT GGCCTGCTGG 
AACGGTTCAA TGCGTCGTTT TGTGTTACGA AAAAAAGGCA GTGACCCATC ATCTAGAGCT 
TGGATCACTT CAATTATAAA ATCCGGAACA AGTGGAAGCT CCACGTCAGT CTCACCAAGT 
CCGTTGACCA AAGATGGACA CGTAAAGAGT GCATCAAGCA ATCAACTTCA TGGACGATCA 
TTGGACACTG ACGCTTTTGG AGAGCACCTT GAAGTGACAG AAGGGTTGAA CCCTCGTGCA 
AGATCAATGG GCGACACATT TCTGGTATGT GTTCACAATG TTAGTGAAGA TCAGCCTTAT 
GCAATCCTAC GTGCTGGAAT CCACAGCACT GCCGCCGACA TTATTCGTCA GGTGTTTGTT 
AAAGCTCGCC GTTCAAATGT AGACGATTCC GAGTTTGTTC TTGTTGAAGA AACCTGCGAT 
GATCCAAAAC TCAATCAAGG CCAAATGACT CCAAAGTATC CAAACAACCG CACCACAAGC 
CGAGTGTTAG GACAAAACGA AAATGTTTGG AAAGCACAAT CGAGGTGGAA GTCAATGGGC 
AGATTTGTAC TTGAAAACCG AAAGGACACA GTGCACGCAA CCCTCGAAAA AGTGAGATCA 
TTTATATCAA AACTGGAAGC TGCAAAAGCA AGCATGGATC CCAGACACGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	plc-1
Entrez Gene ID	181274
Full Name	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

Homo sapiens (human)	PLCE1	NP_057425.3
Gallus gallus (chicken)	LOC425224	XP_004942210.1
Danio rerio (zebrafish)	plce1	NP_001155125.1
Bos taurus (cattle)	PLCE1	NP_001192781.1
	PLCE1	XP_002805823.1
Mus musculus (house mouse)	Plce1	NP_062534.2
Rattus norvegicus (Norway rat)	Plce1	NP_446210.1
Caenorhabditis elegans (roundworm)	plc-1	NP_001123141.1
Canis lupus familiaris (dog)	PLCE1	NP_001130037.1
Pan troglodytes (chimpanzee)	PLCE1	XP_001149565.1
Xenopus tropicalis (tropical clawed frog)	plce1	XP_002937222.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

plc-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

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