PLCE1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017016312.1	XP_016871801.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X5
XM_006717885.3	XP_006717948.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
XM_017016310.1	XP_016871799.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
XM_011539850.3	XP_011538152.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X6
XM_017016311.2	XP_016871800.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
NM_016341.4	NP_057425.3	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform 1
XM_011539850.2	XP_011538152.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X6
XM_006717885.4	XP_006717948.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
XM_017016310.2	XP_016871799.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
XM_017016312.2	XP_016871801.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X5
NM_016341.3	NP_057425.3	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform 1
XM_006717889.3	XP_006717952.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
XM_011539851.2	XP_011538153.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X7
XM_011539852.2	XP_011538154.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X8
XM_006717888.3	XP_006717951.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
XM_011539849.2	XP_011538151.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
XM_011539849.3	XP_011538151.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
XM_006717888.4	XP_006717951.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
NM_001165979.2	NP_001159451.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform 2
NM_001288989.2	NP_001275918.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform 3
NM_001288989.1	NP_001275918.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform 3
XM_006717889.4	XP_006717952.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
XM_017016311.1	XP_016871800.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
XM_006717890.2	XP_006717953.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X4
XM_006717890.3	XP_006717953.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X4
XM_011539851.3	XP_011538153.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X7
XM_011539852.3	XP_011538154.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu91455	XM_017016312.2 Latest version!	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu27663	NM_001165979.2 Latest version!	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu67755	XM_011539850.3 Latest version!	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu67756	XM_011539851.3 Latest version!	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu67757	XM_011539852.3 Latest version!	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu25512	NM_016341.4 Latest version!	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu27664	NM_001288989.2 Latest version!	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu44365	XM_017016311.2 Latest version!	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu44365	XM_017016310.2 Latest version!	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu44365	XM_006717885.4 Latest version!	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu44365	XM_011539849.3 Latest version!	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu44366	XM_006717888.4 Latest version!	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu44367	XM_006717889.4 Latest version!	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu44368	XM_006717890.3 Latest version!	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu67755	XM_011539850.2	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu44365	XM_006717885.3	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu44365	XM_017016310.1	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu91455	XM_017016312.1	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu25512	NM_016341.3	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu44365	XM_011539849.2	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu44366	XM_006717888.3	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu27664	NM_001288989.1	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu44367	XM_006717889.3	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu44365	XM_017016311.1	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu44368	XM_006717890.2	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu67756	XM_011539851.2	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu67757	XM_011539852.2	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

PLCE1 ( XM_017016312.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871801.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu91455
Clone ID Related Accession (Same CDS sequence)	XM_017016312.1 , XM_017016312.2
Accession Version	XM_017016312.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCAG AAGGAAGTGC AGCAGGAAGA GACTTTGCAG GAATGGAAGA GGTGAGGCAG 
CTCCACGTGA GATTCTGCAA AGGGATTAAG ATTTGGCACC AGGCTTGGTT TCTGTGCAGC 
CTCTTAGGCA GAGAGCCCCA GGAGAGGGAA GCAGGCTGTC AGCTGTGGCT TTGCACGCTC 
TCAGCTGTCT TGAAGGTTGG TTGGCTCTTC CCGCTCTCTG AGGTACCCAA TTTTACCTTG 
TTAAAGGATG GATGTGGATG CTGGAGGCTT AAAGAAGACC AGATCTACAA TGCAGTGAGA 
AGAGAAGAAA CAGAAAATAC AGTTGGATCT CTACTCCATT TCCTCACCAA GCTCCCAGCC 
TCCGAGACAG CCCATGGAAG GATAAGCGTT GGTCCATGCT TAAAGCAATG TGTCCGAGAC 
ACTGTATGTG AGTATCGCGC CACCCTCCAA AGGACTTCAA TATCGCAGTA CATCACCGGT 
TCTCTCCTAG AAGCAACCAC GTCTTTGGGA GCAAGAAGTG GCCTTCTCAG TACTTTTGGA 
GGATCCACTG GACGAATGAT GCTGAAAGAA CGCCAGCCAG GCCCCTCTGT GGCCAATTCC 
AATGCCCTCC CTTCAAGTTC AGCTGGGATC AGCAAGGAGC TGATCGATCT GCAGCCTCTC 
ATCCAGTTCC CAGAGGAAGT CGCCAGCATC CTGATGGAGC AAGAGCAGAC TATTTACCGC 
AGGGTCTTGC CAGTCGACTA CCTTTGCTTC TTAACACGGG ACTTGGGCAC TCCTGAATGC 
CAGAGCTCCT TGCCCTGCCT CAAAGCATCC ATCTCAGCGT CGATTCTTAC CACTCAGAAT 
GGAGAGCACA ATGCCCTTGA AGATCTGGTG ATGAGGTTTA ATGAGGTGAG CTCCTGGGTG 
ACATGGCTGA TCCTCACGGC AGGCTCCATG GAGGAGAAGC GAGAAGTCTT TTCATATTTG 
GTGCATGTGG CCAAATGCTG CTGGAACATG GGCAACTACA ACGCTGTCAT GGAGTTCTTG 
GCTGGCCTCA GGTCAAGAAA AGTTTTAAAA ATGTGGCAGT TCATGGACCA GTCTGATATT 
GAGACCATGA GGAGCCTGAA GGATGCTATG GCCCAGCATG AGTCCTCTTG TGAGTACAGA 
AAGGTGGTGA CACGTGCCCT GCACATCCCT GGCTGTAAGG TGGTTCCATT CTGTGGGGTG 
TTTCTGAAGG AGCTCTGTGA AGTGCTTGAC GGCGCCTCCG GTCTCATGAA GCTTTGCCCG 
CGGTACAATT CCCAAGAAGA AACTTTAGAG TTTGTAGCAG ATTACAGTGG ACAAGATAAT 
TTCTTACAAC GAGTGGGACA AAATGGCTTA AAGAATTCGG AGAAGGAGTC CACTGTCAAC 
AGCATCTTTC AGGTCATCCG GAGCTGCAAT CGAAGTCTGG AGACAGACGA GGAGGACAGC 
CCCAGTGAAG GAAACAGCTC CAGGAAAAGC TCCTTGAAGG ATAAAAGCCG ATGGCAGTTT 
ATAATTGGAG ATTTGTTGGA TTCAGACAAT GACATCTTTG AGCAATCCAA AGAATACGAC 
TCTCATGGTT CAGAGGACTC ACAGAAGGCC TTCGACCATG GGACGGAGCT CATCCCTTGG 
TACGTGCTGT CCATCCAAGC CGATGTGCAC CAGTTCCTGC TGCAGGGGGC CACGGTCATC 
CACTACGACC AGGACACACA CCTCTCTGCC CGCTGCTTCC TCCAGCTTCA GCCCGACAAT 
AGCACCTTGA CCTGGGTAAA GCCCACAACT GCCTCCCCAG CCAGCAGTAA AGCAAAACTT 
GGTGTACTTA ATAACACAGC TGAGCCTGGA AAATTCCCAC TACTGGGTAA TGCTGGATTA 
AGTAGCCTGA CGGAAGGGGT CTTGGATCTT TTTGCAGTGA AGGCTGTATA CATGGGCCAC 
CCTGGCATTG ATATACACAC TGTGTGTGTT CAGAACAAAC TGGGTAGCAT GTTCCTGTCA 
GAGACTGGTG TGACATTGCT CTATGGGCTT CAGACCACAG ACAACAGATT ATTGCACTTC 
GTGGCACCAA AGCACACAGC TAAAATGCTC TTCAGCGGAT TATTGGAACT CACTAGAGCT 
GTGAGAAAGA TGAGGAAATT CCCTGACCAA AGACAGCAGT GGCTGCGGAA ACAGTACGTC 
AGCCTTTATC AGGAGGATGG ACGGTATGAA GGCCCAACTT TGGCTCACGC TGTGGAGTTG 
TTTGGTGGCA GACGGTGGAG TGCTCGAAAC CCCAGCCCCG GAACATCAGC AAAGAATGCT 
GAGAAGCCCA ATATGCAGAG AAACAATACC CTGGGCATAA GCACTACCAA GAAAAAGAAG 
AAAATCCTCA TGAGGGGTGA GAGTGGAGAG GTAACTGACG ATGAGATGGC AACCCGAAAG 
GCCAAGATGC ACAAAGAGTG TCGAAGCCGG AGTGGTTCTG ATCCTCAAGA CATTAATGAA 
CAAGAAGAAT CAGAGGTGAA TGCCATCGCT AACCCTCCAA ACCCCCTCCC TTCCAGAAGA 
GCCCACTCTT TGACCACAGC TGGGTCCCCC AACTTGGCTG CCGGGACGTC ATCTCCCATC 
AGTGCTTGGA GCAGTAGTAG CTGGCACGGG CGGATCAAAG GCGGCATGAA GGGATTTCAG 
AGCTTCATGG TTTCAGATAG CAACATGAGT TTTGTTGAAT TTGTTGAGCT GTTCAAATCA 
TTCAGTGTCA GGAGCCGCAA GGACCTGAAG GATCTGTTTG ATGTCTATGC AGTGCCCTGC 
AACCGATCTG GCTCCGAGTC AGCCCCACTC TACACCAACC TGACAATTGA TGAAAACACC 
AGCGATCTTC AGCCTGACCT AGATCTGTTG ACCAGAAATG TCTCGGATTT GGGGTTGTTC 
ATTAAGAGTA AACAGCAGCT ATCGGACAAC CAGAGGCAGA TATCTGATGC CATTGCTGCT 
GCAAGCATTG TGACAAATGG CACTGGGATT GAGAGCACAT CTCTGGGCAT TTTTGGGGTG 
GGCATACTTC AGCTCAACGA TTTCCTCGTG AATTGCCAAG GAGAACACTG CACTTATGAT 
GAAATCCTCA GCATCATCCA GAAGTTCGAG CCTAGCATCA GTATGTGTCA TCAGGGACTA 
ATGTCATTTG AAGGGTTTGC CAGGTTTCTG ATGGATAAAG AAAATTTTGC CTCAAAAAAT 
GATGAGTCAC AGGAGAACAT TAAAGAACTG CAGCTACCCC TCTCATACTA TTACATCGAA 
TCTTCGCACA ATACCTACCT CACGGGCCAT CAGCTCAAAG GAGAATCCTC GGTAGAACTC 
TACAGCCAGG TCCTTTTGCA AGGCTGTCGA AGTGTAGAAT TGGACTGCTG GGACGGAGAC 
GATGGGATGC CCATCATTTA TCATGGACAT ACGCTGACAA CCAAGATCCC CTTCAAGGAA 
GTGGTTGAAG CCATTGATCG CAGTGCCTTC ATCAACTCTG ACCTGCCAAT CATCATATCG 
ATTGAGAACC ACTGTTCATT GCCTCAGCAA CGAAAAATGG CAGAAATTTT CAAGACTGTG 
TTTGGAGAAA AGCTGGTGAC TAAATTCTTA TTTGAGACTG ATTTCTCAGA TGATCCAATG 
CTTCCTTCAC CTGACCAACT CAGAAAGAAA GTTCTTCTTA AAAACAAGAA GCTAAAAGCC 
CATCAGACGC CAGTGGATAT CTTAAAGCAA AAGGCTCATC AGTTAGCATC TATGCAAGTG 
CAGGCTTATA ATGGTGGGAA TGCCAACCCC CGACCTGCCA ATAATGAGGA AGAGGAAGAT 
GAGGAGGACG AATATGATTA TGACTATGAA TCCCTTTCTG ATGACAACAT TCTGGAAGAC 
AGACCTGAAA ATAAATCATG TAATGACAAG CTTCAGTTTG AATATAATGA AGAAATCCCA 
AAGAGGATAA AGAAAGCAGA TAACTCTGCT TGCAACAAAG GAAAGGTTTA TGATATGGAA 
CTGGGAGAAG AATTTTATCT TGATCAGAAT AAAAAGGAAA GCAGACAGAT TGCACCAGAG 
CTTTCTGACC TTGTAATCTA TTGTCAAGCA GTAAAATTTC CAGGACTGTC AACTCTAAAT 
GCATCTGGCT CTAGCAGAGG AAAAGAAAGG AAAAGCAGGA AGTCCATTTT TGGCAACAAT 
CCGGGCAGAA TGAGCCCAGG GGAGACAGCA TCATTTAACA AAACATCTGG AAAAAGTTCC 
TGTGAAGGCA TTCGACAGAC CTGGGAGGAA TCTTCTTCCC CTCTCAACCC AACCACGTCC 
CTCAGTGCTA TCATTAGAAC TCCCAAATGT TATCATATCT CGTCGCTGAA TGAAAATGCC 
GCCAAACGTC TGTGTCGCAG GTATTCTCAG AAACTGACCC AGCACACCGC CTGTCAGCTG 
CTGAGAACTT ACCCTGCTGC CACCCGCATC GACTCTTCCA ACCCGAACCC CCTCATGTTC 
TGGCTCCATG GGATACAGCT TGTGGCACTC AACTACCAGA CTGATGATCT CCCTTTACAT 
TTAAATGCTG CAATGTTTGA GGCAAATGGT GGTTGTGGTT ATGTATTGAA ACCTCCAGTT 
CTGTGGGACA AGAACTGCCC CATGTATCAG AAGTTTTCTC CACTAGAAAG AGATCTGGAC 
AGCATGGATC CTGCAGTCTA TTCTTTAACT ATTGTCTCTG GTCAGAATGT GTGCCCCAGT 
AATAGCATGG GAAGCCCGTG CATTGAAGTC GACGTCCTGG GCATGCCTCT GGACAGCTGC 
CATTTCCGCA CAAAGCCCAT CCATCGAAAC ACCCTGAACC CCATGTGGAA CGAGCAGTTT 
CTGTTCCACG TTCACTTCGA AGATCTTGTA TTTCTTCGTT TTGCAGTTGT GGAAAACAAT 
AGTTCAGCGG TAACTGCTCA GAGAATCATT CCACTGAAAG CTTTAAAACG AGGATATCGA 
CATCTTCAGC TGCGAAACCT TCACAATGAA GTCTTGGAGA TTTCTAGTTT ATTCATTAAC 
AGCAGAAGGA TGGAAGAAAA TTCCTCTGGC AATACCATGT CAGCCTCTTC GATGTTTAAT 
ACAGAAGAAA GAAAATGTTT GCAGACTCAC AGAGTCACGG TGCATGGGGT CCCAGGGCCA 
GAGCCCTTTA CCGTTTTCAC TATTAATGGA GGCACCAAGG CAAAGCAGCT TCTGCAGCAA 
ATTCTGACAA ATGAACAAGA CATCAAACCT GTTACCACAG ACTATTTTTT GATGGAAGAA 
AAATATTTTA TATCTAAAGA AAAGAATGAA TGTAGGAAAC AACCATTCCA GAGAGCCATT 
GGTCCAGAAG AGGAGATCAT GCAAATTTTA AGCAGCTGGT TTCCAGAAGA GGGATACATG 
GGCAGGATTG TCTTAAAAAC CCAGCAGGAA AACCTAGAAG AGAAAAACAT TGTTCAAGAT 
GACAAAGAGG TGATCTTGAG CTCAGAGGAG GAGAGTTTCT TTGTCCAAGT GCATGATGTT 
TCTCCAGAGC AACCTCGAAC AGTCATCAAA GCACCCCGCG TCAGCACTGC ACAGGATGTC 
ATTCAGCAGA CCTTATGCAA AGCCAAATAT TCCTACAGCA TCCTGAGCAA CCCCAATCCA 
AGCGACTATG TGCTTTTGGA AGAGGTGGTG AAAGACACTA CCAACAAGAA GACTACCACA 
CCAAAGTCCT CTCAGCGGGT CCTTCTGGAT CAGGAGTGTG TGTTTCAAGC CCAAAGCAAG 
TGGAAAGGTG CAGGAAAATT CATCCTTAAG CTAAAGGAGC AGGTGCAGGC ATCTCGAGAA 
GATAAAAAGA AAGGCATTTC TTTCGCAAGT GAACTCAAGA AGCTCACCAA GTCAACTAAA 
CAGCCCCGAG GACTTACATC ACCTTCTCAG CTCTTGACCT CAGAAAGTAT CCAAACCAAG 
GAGGAGAAAC CTGTGGGTGG CTTGTCCTCC AGTGACACAA TGGATTACCG ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871801.1
CDS	23..5959
Translation	MVSEGSAAGRDFAGMEEVRQLHVRFCKGIKIWHQAWFLCSLLGREPQEREAGCQLWLCTLSAVLKVGWLFPLSEVPNFTLLKDGCGCWRLKEDQIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_017016312.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016312.1

ORF Insert Sequence:

ATGGTTTCAG AAGGAAGTGC AGCAGGAAGA GACTTTGCAG GAATGGAAGA GGTGAGGCAG 
CTCCACGTGA GATTCTGCAA AGGGATTAAG ATTTGGCACC AGGCTTGGTT TCTGTGCAGC 
CTCTTAGGCA GAGAGCCCCA GGAGAGGGAA GCAGGCTGTC AGCTGTGGCT TTGCACGCTC 
TCAGCTGTCT TGAAGGTTGG TTGGCTCTTC CCGCTCTCTG AGGTACCCAA TTTTACCTTG 
TTAAAGGATG GATGTGGATG CTGGAGGCTT AAAGAAGACC AGATCTACAA TGCAGTGAGA 
AGAGAAGAAA CAGAAAATAC AGTTGGATCT CTACTCCATT TCCTCACCAA GCTCCCAGCC 
TCCGAGACAG CCCATGGAAG GATAAGCGTT GGTCCATGCT TAAAGCAATG TGTCCGAGAC 
ACTGTATGTG AGTATCGCGC CACCCTCCAA AGGACTTCAA TATCGCAGTA CATCACCGGT 
TCTCTCCTAG AAGCAACCAC GTCTTTGGGA GCAAGAAGTG GCCTTCTCAG TACTTTTGGA 
GGATCCACTG GACGAATGAT GCTGAAAGAA CGCCAGCCAG GCCCCTCTGT GGCCAATTCC 
AATGCCCTCC CTTCAAGTTC AGCTGGGATC AGCAAGGAGC TGATCGATCT GCAGCCTCTC 
ATCCAGTTCC CAGAGGAAGT CGCCAGCATC CTGATGGAGC AAGAGCAGAC TATTTACCGC 
AGGGTCTTGC CAGTCGACTA CCTTTGCTTC TTAACACGGG ACTTGGGCAC TCCTGAATGC 
CAGAGCTCCT TGCCCTGCCT CAAAGCATCC ATCTCAGCGT CGATTCTTAC CACTCAGAAT 
GGAGAGCACA ATGCCCTTGA AGATCTGGTG ATGAGGTTTA ATGAGGTGAG CTCCTGGGTG 
ACATGGCTGA TCCTCACGGC AGGCTCCATG GAGGAGAAGC GAGAAGTCTT TTCATATTTG 
GTGCATGTGG CCAAATGCTG CTGGAACATG GGCAACTACA ACGCTGTCAT GGAGTTCTTG 
GCTGGCCTCA GGTCAAGAAA AGTTTTAAAA ATGTGGCAGT TCATGGACCA GTCTGATATT 
GAGACCATGA GGAGCCTGAA GGATGCTATG GCCCAGCATG AGTCCTCTTG TGAGTACAGA 
AAGGTGGTGA CACGTGCCCT GCACATCCCT GGCTGTAAGG TGGTTCCATT CTGTGGGGTG 
TTTCTGAAGG AGCTCTGTGA AGTGCTTGAC GGCGCCTCCG GTCTCATGAA GCTTTGCCCG 
CGGTACAATT CCCAAGAAGA AACTTTAGAG TTTGTAGCAG ATTACAGTGG ACAAGATAAT 
TTCTTACAAC GAGTGGGACA AAATGGCTTA AAGAATTCGG AGAAGGAGTC CACTGTCAAC 
AGCATCTTTC AGGTCATCCG GAGCTGCAAT CGAAGTCTGG AGACAGACGA GGAGGACAGC 
CCCAGTGAAG GAAACAGCTC CAGGAAAAGC TCCTTGAAGG ATAAAAGCCG ATGGCAGTTT 
ATAATTGGAG ATTTGTTGGA TTCAGACAAT GACATCTTTG AGCAATCCAA AGAATACGAC 
TCTCATGGTT CAGAGGACTC ACAGAAGGCC TTCGACCATG GGACGGAGCT CATCCCTTGG 
TACGTGCTGT CCATCCAAGC CGATGTGCAC CAGTTCCTGC TGCAGGGGGC CACGGTCATC 
CACTACGACC AGGACACACA CCTCTCTGCC CGCTGCTTCC TCCAGCTTCA GCCCGACAAT 
AGCACCTTGA CCTGGGTAAA GCCCACAACT GCCTCCCCAG CCAGCAGTAA AGCAAAACTT 
GGTGTACTTA ATAACACAGC TGAGCCTGGA AAATTCCCAC TACTGGGTAA TGCTGGATTA 
AGTAGCCTGA CGGAAGGGGT CTTGGATCTT TTTGCAGTGA AGGCTGTATA CATGGGCCAC 
CCTGGCATTG ATATACACAC TGTGTGTGTT CAGAACAAAC TGGGTAGCAT GTTCCTGTCA 
GAGACTGGTG TGACATTGCT CTATGGGCTT CAGACCACAG ACAACAGATT ATTGCACTTC 
GTGGCACCAA AGCACACAGC TAAAATGCTC TTCAGCGGAT TATTGGAACT CACTAGAGCT 
GTGAGAAAGA TGAGGAAATT CCCTGACCAA AGACAGCAGT GGCTGCGGAA ACAGTACGTC 
AGCCTTTATC AGGAGGATGG ACGGTATGAA GGCCCAACTT TGGCTCACGC TGTGGAGTTG 
TTTGGTGGCA GACGGTGGAG TGCTCGAAAC CCCAGCCCCG GAACATCAGC AAAGAATGCT 
GAGAAGCCCA ATATGCAGAG AAACAATACC CTGGGCATAA GCACTACCAA GAAAAAGAAG 
AAAATCCTCA TGAGGGGTGA GAGTGGAGAG GTAACTGACG ATGAGATGGC AACCCGAAAG 
GCCAAGATGC ACAAAGAGTG TCGAAGCCGG AGTGGTTCTG ATCCTCAAGA CATTAATGAA 
CAAGAAGAAT CAGAGGTGAA TGCCATCGCT AACCCTCCAA ACCCCCTCCC TTCCAGAAGA 
GCCCACTCTT TGACCACAGC TGGGTCCCCC AACTTGGCTG CCGGGACGTC ATCTCCCATC 
AGTGCTTGGA GCAGTAGTAG CTGGCACGGG CGGATCAAAG GCGGCATGAA GGGATTTCAG 
AGCTTCATGG TTTCAGATAG CAACATGAGT TTTGTTGAAT TTGTTGAGCT GTTCAAATCA 
TTCAGTGTCA GGAGCCGCAA GGACCTGAAG GATCTGTTTG ATGTCTATGC AGTGCCCTGC 
AACCGATCTG GCTCCGAGTC AGCCCCACTC TACACCAACC TGACAATTGA TGAAAACACC 
AGCGATCTTC AGCCTGACCT AGATCTGTTG ACCAGAAATG TCTCGGATTT GGGGTTGTTC 
ATTAAGAGTA AACAGCAGCT ATCGGACAAC CAGAGGCAGA TATCTGATGC CATTGCTGCT 
GCAAGCATTG TGACAAATGG CACTGGGATT GAGAGCACAT CTCTGGGCAT TTTTGGGGTG 
GGCATACTTC AGCTCAACGA TTTCCTCGTG AATTGCCAAG GAGAACACTG CACTTATGAT 
GAAATCCTCA GCATCATCCA GAAGTTCGAG CCTAGCATCA GTATGTGTCA TCAGGGACTA 
ATGTCATTTG AAGGGTTTGC CAGGTTTCTG ATGGATAAAG AAAATTTTGC CTCAAAAAAT 
GATGAGTCAC AGGAGAACAT TAAAGAACTG CAGCTACCCC TCTCATACTA TTACATCGAA 
TCTTCGCACA ATACCTACCT CACGGGCCAT CAGCTCAAAG GAGAATCCTC GGTAGAACTC 
TACAGCCAGG TCCTTTTGCA AGGCTGTCGA AGTGTAGAAT TGGACTGCTG GGACGGAGAC 
GATGGGATGC CCATCATTTA TCATGGACAT ACGCTGACAA CCAAGATCCC CTTCAAGGAA 
GTGGTTGAAG CCATTGATCG CAGTGCCTTC ATCAACTCTG ACCTGCCAAT CATCATATCG 
ATTGAGAACC ACTGTTCATT GCCTCAGCAA CGAAAAATGG CAGAAATTTT CAAGACTGTG 
TTTGGAGAAA AGCTGGTGAC TAAATTCTTA TTTGAGACTG ATTTCTCAGA TGATCCAATG 
CTTCCTTCAC CTGACCAACT CAGAAAGAAA GTTCTTCTTA AAAACAAGAA GCTAAAAGCC 
CATCAGACGC CAGTGGATAT CTTAAAGCAA AAGGCTCATC AGTTAGCATC TATGCAAGTG 
CAGGCTTATA ATGGTGGGAA TGCCAACCCC CGACCTGCCA ATAATGAGGA AGAGGAAGAT 
GAGGAGGACG AATATGATTA TGACTATGAA TCCCTTTCTG ATGACAACAT TCTGGAAGAC 
AGACCTGAAA ATAAATCATG TAATGACAAG CTTCAGTTTG AATATAATGA AGAAATCCCA 
AAGAGGATAA AGAAAGCAGA TAACTCTGCT TGCAACAAAG GAAAGGTTTA TGATATGGAA 
CTGGGAGAAG AATTTTATCT TGATCAGAAT AAAAAGGAAA GCAGACAGAT TGCACCAGAG 
CTTTCTGACC TTGTAATCTA TTGTCAAGCA GTAAAATTTC CAGGACTGTC AACTCTAAAT 
GCATCTGGCT CTAGCAGAGG AAAAGAAAGG AAAAGCAGGA AGTCCATTTT TGGCAACAAT 
CCGGGCAGAA TGAGCCCAGG GGAGACAGCA TCATTTAACA AAACATCTGG AAAAAGTTCC 
TGTGAAGGCA TTCGACAGAC CTGGGAGGAA TCTTCTTCCC CTCTCAACCC AACCACGTCC 
CTCAGTGCTA TCATTAGAAC TCCCAAATGT TATCATATCT CGTCGCTGAA TGAAAATGCC 
GCCAAACGTC TGTGTCGCAG GTATTCTCAG AAACTGACCC AGCACACCGC CTGTCAGCTG 
CTGAGAACTT ACCCTGCTGC CACCCGCATC GACTCTTCCA ACCCGAACCC CCTCATGTTC 
TGGCTCCATG GGATACAGCT TGTGGCACTC AACTACCAGA CTGATGATCT CCCTTTACAT 
TTAAATGCTG CAATGTTTGA GGCAAATGGT GGTTGTGGTT ATGTATTGAA ACCTCCAGTT 
CTGTGGGACA AGAACTGCCC CATGTATCAG AAGTTTTCTC CACTAGAAAG AGATCTGGAC 
AGCATGGATC CTGCAGTCTA TTCTTTAACT ATTGTCTCTG GTCAGAATGT GTGCCCCAGT 
AATAGCATGG GAAGCCCGTG CATTGAAGTC GACGTCCTGG GCATGCCTCT GGACAGCTGC 
CATTTCCGCA CAAAGCCCAT CCATCGAAAC ACCCTGAACC CCATGTGGAA CGAGCAGTTT 
CTGTTCCACG TTCACTTCGA AGATCTTGTA TTTCTTCGTT TTGCAGTTGT GGAAAACAAT 
AGTTCAGCGG TAACTGCTCA GAGAATCATT CCACTGAAAG CTTTAAAACG AGGATATCGA 
CATCTTCAGC TGCGAAACCT TCACAATGAA GTCTTGGAGA TTTCTAGTTT ATTCATTAAC 
AGCAGAAGGA TGGAAGAAAA TTCCTCTGGC AATACCATGT CAGCCTCTTC GATGTTTAAT 
ACAGAAGAAA GAAAATGTTT GCAGACTCAC AGAGTCACGG TGCATGGGGT CCCAGGGCCA 
GAGCCCTTTA CCGTTTTCAC TATTAATGGA GGCACCAAGG CAAAGCAGCT TCTGCAGCAA 
ATTCTGACAA ATGAACAAGA CATCAAACCT GTTACCACAG ACTATTTTTT GATGGAAGAA 
AAATATTTTA TATCTAAAGA AAAGAATGAA TGTAGGAAAC AACCATTCCA GAGAGCCATT 
GGTCCAGAAG AGGAGATCAT GCAAATTTTA AGCAGCTGGT TTCCAGAAGA GGGATACATG 
GGCAGGATTG TCTTAAAAAC CCAGCAGGAA AACCTAGAAG AGAAAAACAT TGTTCAAGAT 
GACAAAGAGG TGATCTTGAG CTCAGAGGAG GAGAGTTTCT TTGTCCAAGT GCATGATGTT 
TCTCCAGAGC AACCTCGAAC AGTCATCAAA GCACCCCGCG TCAGCACTGC ACAGGATGTC 
ATTCAGCAGA CCTTATGCAA AGCCAAATAT TCCTACAGCA TCCTGAGCAA CCCCAATCCA 
AGCGACTATG TGCTTTTGGA AGAGGTGGTG AAAGACACTA CCAACAAGAA GACTACCACA 
CCAAAGTCCT CTCAGCGGGT CCTTCTGGAT CAGGAGTGTG TGTTTCAAGC CCAAAGCAAG 
TGGAAAGGTG CAGGAAAATT CATCCTTAAG CTAAAGGAGC AGGTGCAGGC ATCTCGAGAA 
GATAAAAAGA AAGGCATTTC TTTCGCAAGT GAACTCAAGA AGCTCACCAA GTCAACTAAA 
CAGCCCCGAG GACTTACATC ACCTTCTCAG CTCTTGACCT CAGAAAGTAT CCAAACCAAG 
GAGGAGAAAC CTGTGGGTGG CTTGTCCTCC AGTGACACAA TGGATTACCG ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_017016312.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871801.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X8, mRNA.

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CloneID	OHu91455
Clone ID Related Accession (Same CDS sequence)	XM_017016312.1 , XM_017016312.2
Accession Version	XM_017016312.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016312.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCAG AAGGAAGTGC AGCAGGAAGA GACTTTGCAG GAATGGAAGA GGTGAGGCAG 
CTCCACGTGA GATTCTGCAA AGGGATTAAG ATTTGGCACC AGGCTTGGTT TCTGTGCAGC 
CTCTTAGGCA GAGAGCCCCA GGAGAGGGAA GCAGGCTGTC AGCTGTGGCT TTGCACGCTC 
TCAGCTGTCT TGAAGGTTGG TTGGCTCTTC CCGCTCTCTG AGGTACCCAA TTTTACCTTG 
TTAAAGGATG GATGTGGATG CTGGAGGCTT AAAGAAGACC AGATCTACAA TGCAGTGAGA 
AGAGAAGAAA CAGAAAATAC AGTTGGATCT CTACTCCATT TCCTCACCAA GCTCCCAGCC 
TCCGAGACAG CCCATGGAAG GATAAGCGTT GGTCCATGCT TAAAGCAATG TGTCCGAGAC 
ACTGTATGTG AGTATCGCGC CACCCTCCAA AGGACTTCAA TATCGCAGTA CATCACCGGT 
TCTCTCCTAG AAGCAACCAC GTCTTTGGGA GCAAGAAGTG GCCTTCTCAG TACTTTTGGA 
GGATCCACTG GACGAATGAT GCTGAAAGAA CGCCAGCCAG GCCCCTCTGT GGCCAATTCC 
AATGCCCTCC CTTCAAGTTC AGCTGGGATC AGCAAGGAGC TGATCGATCT GCAGCCTCTC 
ATCCAGTTCC CAGAGGAAGT CGCCAGCATC CTGATGGAGC AAGAGCAGAC TATTTACCGC 
AGGGTCTTGC CAGTCGACTA CCTTTGCTTC TTAACACGGG ACTTGGGCAC TCCTGAATGC 
CAGAGCTCCT TGCCCTGCCT CAAAGCATCC ATCTCAGCGT CGATTCTTAC CACTCAGAAT 
GGAGAGCACA ATGCCCTTGA AGATCTGGTG ATGAGGTTTA ATGAGGTGAG CTCCTGGGTG 
ACATGGCTGA TCCTCACGGC AGGCTCCATG GAGGAGAAGC GAGAAGTCTT TTCATATTTG 
GTGCATGTGG CCAAATGCTG CTGGAACATG GGCAACTACA ACGCTGTCAT GGAGTTCTTG 
GCTGGCCTCA GGTCAAGAAA AGTTTTAAAA ATGTGGCAGT TCATGGACCA GTCTGATATT 
GAGACCATGA GGAGCCTGAA GGATGCTATG GCCCAGCATG AGTCCTCTTG TGAGTACAGA 
AAGGTGGTGA CACGTGCCCT GCACATCCCT GGCTGTAAGG TGGTTCCATT CTGTGGGGTG 
TTTCTGAAGG AGCTCTGTGA AGTGCTTGAC GGCGCCTCCG GTCTCATGAA GCTTTGCCCG 
CGGTACAATT CCCAAGAAGA AACTTTAGAG TTTGTAGCAG ATTACAGTGG ACAAGATAAT 
TTCTTACAAC GAGTGGGACA AAATGGCTTA AAGAATTCGG AGAAGGAGTC CACTGTCAAC 
AGCATCTTTC AGGTCATCCG GAGCTGCAAT CGAAGTCTGG AGACAGACGA GGAGGACAGC 
CCCAGTGAAG GAAACAGCTC CAGGAAAAGC TCCTTGAAGG ATAAAAGCCG ATGGCAGTTT 
ATAATTGGAG ATTTGTTGGA TTCAGACAAT GACATCTTTG AGCAATCCAA AGAATACGAC 
TCTCATGGTT CAGAGGACTC ACAGAAGGCC TTCGACCATG GGACGGAGCT CATCCCTTGG 
TACGTGCTGT CCATCCAAGC CGATGTGCAC CAGTTCCTGC TGCAGGGGGC CACGGTCATC 
CACTACGACC AGGACACACA CCTCTCTGCC CGCTGCTTCC TCCAGCTTCA GCCCGACAAT 
AGCACCTTGA CCTGGGTAAA GCCCACAACT GCCTCCCCAG CCAGCAGTAA AGCAAAACTT 
GGTGTACTTA ATAACACAGC TGAGCCTGGA AAATTCCCAC TACTGGGTAA TGCTGGATTA 
AGTAGCCTGA CGGAAGGGGT CTTGGATCTT TTTGCAGTGA AGGCTGTATA CATGGGCCAC 
CCTGGCATTG ATATACACAC TGTGTGTGTT CAGAACAAAC TGGGTAGCAT GTTCCTGTCA 
GAGACTGGTG TGACATTGCT CTATGGGCTT CAGACCACAG ACAACAGATT ATTGCACTTC 
GTGGCACCAA AGCACACAGC TAAAATGCTC TTCAGCGGAT TATTGGAACT CACTAGAGCT 
GTGAGAAAGA TGAGGAAATT CCCTGACCAA AGACAGCAGT GGCTGCGGAA ACAGTACGTC 
AGCCTTTATC AGGAGGATGG ACGGTATGAA GGCCCAACTT TGGCTCACGC TGTGGAGTTG 
TTTGGTGGCA GACGGTGGAG TGCTCGAAAC CCCAGCCCCG GAACATCAGC AAAGAATGCT 
GAGAAGCCCA ATATGCAGAG AAACAATACC CTGGGCATAA GCACTACCAA GAAAAAGAAG 
AAAATCCTCA TGAGGGGTGA GAGTGGAGAG GTAACTGACG ATGAGATGGC AACCCGAAAG 
GCCAAGATGC ACAAAGAGTG TCGAAGCCGG AGTGGTTCTG ATCCTCAAGA CATTAATGAA 
CAAGAAGAAT CAGAGGTGAA TGCCATCGCT AACCCTCCAA ACCCCCTCCC TTCCAGAAGA 
GCCCACTCTT TGACCACAGC TGGGTCCCCC AACTTGGCTG CCGGGACGTC ATCTCCCATC 
AGTGCTTGGA GCAGTAGTAG CTGGCACGGG CGGATCAAAG GCGGCATGAA GGGATTTCAG 
AGCTTCATGG TTTCAGATAG CAACATGAGT TTTGTTGAAT TTGTTGAGCT GTTCAAATCA 
TTCAGTGTCA GGAGCCGCAA GGACCTGAAG GATCTGTTTG ATGTCTATGC AGTGCCCTGC 
AACCGATCTG GCTCCGAGTC AGCCCCACTC TACACCAACC TGACAATTGA TGAAAACACC 
AGCGATCTTC AGCCTGACCT AGATCTGTTG ACCAGAAATG TCTCGGATTT GGGGTTGTTC 
ATTAAGAGTA AACAGCAGCT ATCGGACAAC CAGAGGCAGA TATCTGATGC CATTGCTGCT 
GCAAGCATTG TGACAAATGG CACTGGGATT GAGAGCACAT CTCTGGGCAT TTTTGGGGTG 
GGCATACTTC AGCTCAACGA TTTCCTCGTG AATTGCCAAG GAGAACACTG CACTTATGAT 
GAAATCCTCA GCATCATCCA GAAGTTCGAG CCTAGCATCA GTATGTGTCA TCAGGGACTA 
ATGTCATTTG AAGGGTTTGC CAGGTTTCTG ATGGATAAAG AAAATTTTGC CTCAAAAAAT 
GATGAGTCAC AGGAGAACAT TAAAGAACTG CAGCTACCCC TCTCATACTA TTACATCGAA 
TCTTCGCACA ATACCTACCT CACGGGCCAT CAGCTCAAAG GAGAATCCTC GGTAGAACTC 
TACAGCCAGG TCCTTTTGCA AGGCTGTCGA AGTGTAGAAT TGGACTGCTG GGACGGAGAC 
GATGGGATGC CCATCATTTA TCATGGACAT ACGCTGACAA CCAAGATCCC CTTCAAGGAA 
GTGGTTGAAG CCATTGATCG CAGTGCCTTC ATCAACTCTG ACCTGCCAAT CATCATATCG 
ATTGAGAACC ACTGTTCATT GCCTCAGCAA CGAAAAATGG CAGAAATTTT CAAGACTGTG 
TTTGGAGAAA AGCTGGTGAC TAAATTCTTA TTTGAGACTG ATTTCTCAGA TGATCCAATG 
CTTCCTTCAC CTGACCAACT CAGAAAGAAA GTTCTTCTTA AAAACAAGAA GCTAAAAGCC 
CATCAGACGC CAGTGGATAT CTTAAAGCAA AAGGCTCATC AGTTAGCATC TATGCAAGTG 
CAGGCTTATA ATGGTGGGAA TGCCAACCCC CGACCTGCCA ATAATGAGGA AGAGGAAGAT 
GAGGAGGACG AATATGATTA TGACTATGAA TCCCTTTCTG ATGACAACAT TCTGGAAGAC 
AGACCTGAAA ATAAATCATG TAATGACAAG CTTCAGTTTG AATATAATGA AGAAATCCCA 
AAGAGGATAA AGAAAGCAGA TAACTCTGCT TGCAACAAAG GAAAGGTTTA TGATATGGAA 
CTGGGAGAAG AATTTTATCT TGATCAGAAT AAAAAGGAAA GCAGACAGAT TGCACCAGAG 
CTTTCTGACC TTGTAATCTA TTGTCAAGCA GTAAAATTTC CAGGACTGTC AACTCTAAAT 
GCATCTGGCT CTAGCAGAGG AAAAGAAAGG AAAAGCAGGA AGTCCATTTT TGGCAACAAT 
CCGGGCAGAA TGAGCCCAGG GGAGACAGCA TCATTTAACA AAACATCTGG AAAAAGTTCC 
TGTGAAGGCA TTCGACAGAC CTGGGAGGAA TCTTCTTCCC CTCTCAACCC AACCACGTCC 
CTCAGTGCTA TCATTAGAAC TCCCAAATGT TATCATATCT CGTCGCTGAA TGAAAATGCC 
GCCAAACGTC TGTGTCGCAG GTATTCTCAG AAACTGACCC AGCACACCGC CTGTCAGCTG 
CTGAGAACTT ACCCTGCTGC CACCCGCATC GACTCTTCCA ACCCGAACCC CCTCATGTTC 
TGGCTCCATG GGATACAGCT TGTGGCACTC AACTACCAGA CTGATGATCT CCCTTTACAT 
TTAAATGCTG CAATGTTTGA GGCAAATGGT GGTTGTGGTT ATGTATTGAA ACCTCCAGTT 
CTGTGGGACA AGAACTGCCC CATGTATCAG AAGTTTTCTC CACTAGAAAG AGATCTGGAC 
AGCATGGATC CTGCAGTCTA TTCTTTAACT ATTGTCTCTG GTCAGAATGT GTGCCCCAGT 
AATAGCATGG GAAGCCCGTG CATTGAAGTC GACGTCCTGG GCATGCCTCT GGACAGCTGC 
CATTTCCGCA CAAAGCCCAT CCATCGAAAC ACCCTGAACC CCATGTGGAA CGAGCAGTTT 
CTGTTCCACG TTCACTTCGA AGATCTTGTA TTTCTTCGTT TTGCAGTTGT GGAAAACAAT 
AGTTCAGCGG TAACTGCTCA GAGAATCATT CCACTGAAAG CTTTAAAACG AGGATATCGA 
CATCTTCAGC TGCGAAACCT TCACAATGAA GTCTTGGAGA TTTCTAGTTT ATTCATTAAC 
AGCAGAAGGA TGGAAGAAAA TTCCTCTGGC AATACCATGT CAGCCTCTTC GATGTTTAAT 
ACAGAAGAAA GAAAATGTTT GCAGACTCAC AGAGTCACGG TGCATGGGGT CCCAGGGCCA 
GAGCCCTTTA CCGTTTTCAC TATTAATGGA GGCACCAAGG CAAAGCAGCT TCTGCAGCAA 
ATTCTGACAA ATGAACAAGA CATCAAACCT GTTACCACAG ACTATTTTTT GATGGAAGAA 
AAATATTTTA TATCTAAAGA AAAGAATGAA TGTAGGAAAC AACCATTCCA GAGAGCCATT 
GGTCCAGAAG AGGAGATCAT GCAAATTTTA AGCAGCTGGT TTCCAGAAGA GGGATACATG 
GGCAGGATTG TCTTAAAAAC CCAGCAGGAA AACCTAGAAG AGAAAAACAT TGTTCAAGAT 
GACAAAGAGG TGATCTTGAG CTCAGAGGAG GAGAGTTTCT TTGTCCAAGT GCATGATGTT 
TCTCCAGAGC AACCTCGAAC AGTCATCAAA GCACCCCGCG TCAGCACTGC ACAGGATGTC 
ATTCAGCAGA CCTTATGCAA AGCCAAATAT TCCTACAGCA TCCTGAGCAA CCCCAATCCA 
AGCGACTATG TGCTTTTGGA AGAGGTGGTG AAAGACACTA CCAACAAGAA GACTACCACA 
CCAAAGTCCT CTCAGCGGGT CCTTCTGGAT CAGGAGTGTG TGTTTCAAGC CCAAAGCAAG 
TGGAAAGGTG CAGGAAAATT CATCCTTAAG CTAAAGGAGC AGGTGCAGGC ATCTCGAGAA 
GATAAAAAGA AAGGCATTTC TTTCGCAAGT GAACTCAAGA AGCTCACCAA GTCAACTAAA 
CAGCCCCGAG GACTTACATC ACCTTCTCAG CTCTTGACCT CAGAAAGTAT CCAAACCAAG 
GAGGAGAAAC CTGTGGGTGG CTTGTCCTCC AGTGACACAA TGGATTACCG ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871801.1
CDS	23..5959
Translation	MVSEGSAAGRDFAGMEEVRQLHVRFCKGIKIWHQAWFLCSLLGREPQEREAGCQLWLCTLSAVLKVGWLFPLSEVPNFTLLKDGCGCWRLKEDQIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_017016312.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016312.2

ORF Insert Sequence:

ATGGTTTCAG AAGGAAGTGC AGCAGGAAGA GACTTTGCAG GAATGGAAGA GGTGAGGCAG 
CTCCACGTGA GATTCTGCAA AGGGATTAAG ATTTGGCACC AGGCTTGGTT TCTGTGCAGC 
CTCTTAGGCA GAGAGCCCCA GGAGAGGGAA GCAGGCTGTC AGCTGTGGCT TTGCACGCTC 
TCAGCTGTCT TGAAGGTTGG TTGGCTCTTC CCGCTCTCTG AGGTACCCAA TTTTACCTTG 
TTAAAGGATG GATGTGGATG CTGGAGGCTT AAAGAAGACC AGATCTACAA TGCAGTGAGA 
AGAGAAGAAA CAGAAAATAC AGTTGGATCT CTACTCCATT TCCTCACCAA GCTCCCAGCC 
TCCGAGACAG CCCATGGAAG GATAAGCGTT GGTCCATGCT TAAAGCAATG TGTCCGAGAC 
ACTGTATGTG AGTATCGCGC CACCCTCCAA AGGACTTCAA TATCGCAGTA CATCACCGGT 
TCTCTCCTAG AAGCAACCAC GTCTTTGGGA GCAAGAAGTG GCCTTCTCAG TACTTTTGGA 
GGATCCACTG GACGAATGAT GCTGAAAGAA CGCCAGCCAG GCCCCTCTGT GGCCAATTCC 
AATGCCCTCC CTTCAAGTTC AGCTGGGATC AGCAAGGAGC TGATCGATCT GCAGCCTCTC 
ATCCAGTTCC CAGAGGAAGT CGCCAGCATC CTGATGGAGC AAGAGCAGAC TATTTACCGC 
AGGGTCTTGC CAGTCGACTA CCTTTGCTTC TTAACACGGG ACTTGGGCAC TCCTGAATGC 
CAGAGCTCCT TGCCCTGCCT CAAAGCATCC ATCTCAGCGT CGATTCTTAC CACTCAGAAT 
GGAGAGCACA ATGCCCTTGA AGATCTGGTG ATGAGGTTTA ATGAGGTGAG CTCCTGGGTG 
ACATGGCTGA TCCTCACGGC AGGCTCCATG GAGGAGAAGC GAGAAGTCTT TTCATATTTG 
GTGCATGTGG CCAAATGCTG CTGGAACATG GGCAACTACA ACGCTGTCAT GGAGTTCTTG 
GCTGGCCTCA GGTCAAGAAA AGTTTTAAAA ATGTGGCAGT TCATGGACCA GTCTGATATT 
GAGACCATGA GGAGCCTGAA GGATGCTATG GCCCAGCATG AGTCCTCTTG TGAGTACAGA 
AAGGTGGTGA CACGTGCCCT GCACATCCCT GGCTGTAAGG TGGTTCCATT CTGTGGGGTG 
TTTCTGAAGG AGCTCTGTGA AGTGCTTGAC GGCGCCTCCG GTCTCATGAA GCTTTGCCCG 
CGGTACAATT CCCAAGAAGA AACTTTAGAG TTTGTAGCAG ATTACAGTGG ACAAGATAAT 
TTCTTACAAC GAGTGGGACA AAATGGCTTA AAGAATTCGG AGAAGGAGTC CACTGTCAAC 
AGCATCTTTC AGGTCATCCG GAGCTGCAAT CGAAGTCTGG AGACAGACGA GGAGGACAGC 
CCCAGTGAAG GAAACAGCTC CAGGAAAAGC TCCTTGAAGG ATAAAAGCCG ATGGCAGTTT 
ATAATTGGAG ATTTGTTGGA TTCAGACAAT GACATCTTTG AGCAATCCAA AGAATACGAC 
TCTCATGGTT CAGAGGACTC ACAGAAGGCC TTCGACCATG GGACGGAGCT CATCCCTTGG 
TACGTGCTGT CCATCCAAGC CGATGTGCAC CAGTTCCTGC TGCAGGGGGC CACGGTCATC 
CACTACGACC AGGACACACA CCTCTCTGCC CGCTGCTTCC TCCAGCTTCA GCCCGACAAT 
AGCACCTTGA CCTGGGTAAA GCCCACAACT GCCTCCCCAG CCAGCAGTAA AGCAAAACTT 
GGTGTACTTA ATAACACAGC TGAGCCTGGA AAATTCCCAC TACTGGGTAA TGCTGGATTA 
AGTAGCCTGA CGGAAGGGGT CTTGGATCTT TTTGCAGTGA AGGCTGTATA CATGGGCCAC 
CCTGGCATTG ATATACACAC TGTGTGTGTT CAGAACAAAC TGGGTAGCAT GTTCCTGTCA 
GAGACTGGTG TGACATTGCT CTATGGGCTT CAGACCACAG ACAACAGATT ATTGCACTTC 
GTGGCACCAA AGCACACAGC TAAAATGCTC TTCAGCGGAT TATTGGAACT CACTAGAGCT 
GTGAGAAAGA TGAGGAAATT CCCTGACCAA AGACAGCAGT GGCTGCGGAA ACAGTACGTC 
AGCCTTTATC AGGAGGATGG ACGGTATGAA GGCCCAACTT TGGCTCACGC TGTGGAGTTG 
TTTGGTGGCA GACGGTGGAG TGCTCGAAAC CCCAGCCCCG GAACATCAGC AAAGAATGCT 
GAGAAGCCCA ATATGCAGAG AAACAATACC CTGGGCATAA GCACTACCAA GAAAAAGAAG 
AAAATCCTCA TGAGGGGTGA GAGTGGAGAG GTAACTGACG ATGAGATGGC AACCCGAAAG 
GCCAAGATGC ACAAAGAGTG TCGAAGCCGG AGTGGTTCTG ATCCTCAAGA CATTAATGAA 
CAAGAAGAAT CAGAGGTGAA TGCCATCGCT AACCCTCCAA ACCCCCTCCC TTCCAGAAGA 
GCCCACTCTT TGACCACAGC TGGGTCCCCC AACTTGGCTG CCGGGACGTC ATCTCCCATC 
AGTGCTTGGA GCAGTAGTAG CTGGCACGGG CGGATCAAAG GCGGCATGAA GGGATTTCAG 
AGCTTCATGG TTTCAGATAG CAACATGAGT TTTGTTGAAT TTGTTGAGCT GTTCAAATCA 
TTCAGTGTCA GGAGCCGCAA GGACCTGAAG GATCTGTTTG ATGTCTATGC AGTGCCCTGC 
AACCGATCTG GCTCCGAGTC AGCCCCACTC TACACCAACC TGACAATTGA TGAAAACACC 
AGCGATCTTC AGCCTGACCT AGATCTGTTG ACCAGAAATG TCTCGGATTT GGGGTTGTTC 
ATTAAGAGTA AACAGCAGCT ATCGGACAAC CAGAGGCAGA TATCTGATGC CATTGCTGCT 
GCAAGCATTG TGACAAATGG CACTGGGATT GAGAGCACAT CTCTGGGCAT TTTTGGGGTG 
GGCATACTTC AGCTCAACGA TTTCCTCGTG AATTGCCAAG GAGAACACTG CACTTATGAT 
GAAATCCTCA GCATCATCCA GAAGTTCGAG CCTAGCATCA GTATGTGTCA TCAGGGACTA 
ATGTCATTTG AAGGGTTTGC CAGGTTTCTG ATGGATAAAG AAAATTTTGC CTCAAAAAAT 
GATGAGTCAC AGGAGAACAT TAAAGAACTG CAGCTACCCC TCTCATACTA TTACATCGAA 
TCTTCGCACA ATACCTACCT CACGGGCCAT CAGCTCAAAG GAGAATCCTC GGTAGAACTC 
TACAGCCAGG TCCTTTTGCA AGGCTGTCGA AGTGTAGAAT TGGACTGCTG GGACGGAGAC 
GATGGGATGC CCATCATTTA TCATGGACAT ACGCTGACAA CCAAGATCCC CTTCAAGGAA 
GTGGTTGAAG CCATTGATCG CAGTGCCTTC ATCAACTCTG ACCTGCCAAT CATCATATCG 
ATTGAGAACC ACTGTTCATT GCCTCAGCAA CGAAAAATGG CAGAAATTTT CAAGACTGTG 
TTTGGAGAAA AGCTGGTGAC TAAATTCTTA TTTGAGACTG ATTTCTCAGA TGATCCAATG 
CTTCCTTCAC CTGACCAACT CAGAAAGAAA GTTCTTCTTA AAAACAAGAA GCTAAAAGCC 
CATCAGACGC CAGTGGATAT CTTAAAGCAA AAGGCTCATC AGTTAGCATC TATGCAAGTG 
CAGGCTTATA ATGGTGGGAA TGCCAACCCC CGACCTGCCA ATAATGAGGA AGAGGAAGAT 
GAGGAGGACG AATATGATTA TGACTATGAA TCCCTTTCTG ATGACAACAT TCTGGAAGAC 
AGACCTGAAA ATAAATCATG TAATGACAAG CTTCAGTTTG AATATAATGA AGAAATCCCA 
AAGAGGATAA AGAAAGCAGA TAACTCTGCT TGCAACAAAG GAAAGGTTTA TGATATGGAA 
CTGGGAGAAG AATTTTATCT TGATCAGAAT AAAAAGGAAA GCAGACAGAT TGCACCAGAG 
CTTTCTGACC TTGTAATCTA TTGTCAAGCA GTAAAATTTC CAGGACTGTC AACTCTAAAT 
GCATCTGGCT CTAGCAGAGG AAAAGAAAGG AAAAGCAGGA AGTCCATTTT TGGCAACAAT 
CCGGGCAGAA TGAGCCCAGG GGAGACAGCA TCATTTAACA AAACATCTGG AAAAAGTTCC 
TGTGAAGGCA TTCGACAGAC CTGGGAGGAA TCTTCTTCCC CTCTCAACCC AACCACGTCC 
CTCAGTGCTA TCATTAGAAC TCCCAAATGT TATCATATCT CGTCGCTGAA TGAAAATGCC 
GCCAAACGTC TGTGTCGCAG GTATTCTCAG AAACTGACCC AGCACACCGC CTGTCAGCTG 
CTGAGAACTT ACCCTGCTGC CACCCGCATC GACTCTTCCA ACCCGAACCC CCTCATGTTC 
TGGCTCCATG GGATACAGCT TGTGGCACTC AACTACCAGA CTGATGATCT CCCTTTACAT 
TTAAATGCTG CAATGTTTGA GGCAAATGGT GGTTGTGGTT ATGTATTGAA ACCTCCAGTT 
CTGTGGGACA AGAACTGCCC CATGTATCAG AAGTTTTCTC CACTAGAAAG AGATCTGGAC 
AGCATGGATC CTGCAGTCTA TTCTTTAACT ATTGTCTCTG GTCAGAATGT GTGCCCCAGT 
AATAGCATGG GAAGCCCGTG CATTGAAGTC GACGTCCTGG GCATGCCTCT GGACAGCTGC 
CATTTCCGCA CAAAGCCCAT CCATCGAAAC ACCCTGAACC CCATGTGGAA CGAGCAGTTT 
CTGTTCCACG TTCACTTCGA AGATCTTGTA TTTCTTCGTT TTGCAGTTGT GGAAAACAAT 
AGTTCAGCGG TAACTGCTCA GAGAATCATT CCACTGAAAG CTTTAAAACG AGGATATCGA 
CATCTTCAGC TGCGAAACCT TCACAATGAA GTCTTGGAGA TTTCTAGTTT ATTCATTAAC 
AGCAGAAGGA TGGAAGAAAA TTCCTCTGGC AATACCATGT CAGCCTCTTC GATGTTTAAT 
ACAGAAGAAA GAAAATGTTT GCAGACTCAC AGAGTCACGG TGCATGGGGT CCCAGGGCCA 
GAGCCCTTTA CCGTTTTCAC TATTAATGGA GGCACCAAGG CAAAGCAGCT TCTGCAGCAA 
ATTCTGACAA ATGAACAAGA CATCAAACCT GTTACCACAG ACTATTTTTT GATGGAAGAA 
AAATATTTTA TATCTAAAGA AAAGAATGAA TGTAGGAAAC AACCATTCCA GAGAGCCATT 
GGTCCAGAAG AGGAGATCAT GCAAATTTTA AGCAGCTGGT TTCCAGAAGA GGGATACATG 
GGCAGGATTG TCTTAAAAAC CCAGCAGGAA AACCTAGAAG AGAAAAACAT TGTTCAAGAT 
GACAAAGAGG TGATCTTGAG CTCAGAGGAG GAGAGTTTCT TTGTCCAAGT GCATGATGTT 
TCTCCAGAGC AACCTCGAAC AGTCATCAAA GCACCCCGCG TCAGCACTGC ACAGGATGTC 
ATTCAGCAGA CCTTATGCAA AGCCAAATAT TCCTACAGCA TCCTGAGCAA CCCCAATCCA 
AGCGACTATG TGCTTTTGGA AGAGGTGGTG AAAGACACTA CCAACAAGAA GACTACCACA 
CCAAAGTCCT CTCAGCGGGT CCTTCTGGAT CAGGAGTGTG TGTTTCAAGC CCAAAGCAAG 
TGGAAAGGTG CAGGAAAATT CATCCTTAAG CTAAAGGAGC AGGTGCAGGC ATCTCGAGAA 
GATAAAAAGA AAGGCATTTC TTTCGCAAGT GAACTCAAGA AGCTCACCAA GTCAACTAAA 
CAGCCCCGAG GACTTACATC ACCTTCTCAG CTCTTGACCT CAGAAAGTAT CCAAACCAAG 
GAGGAGAAAC CTGTGGGTGG CTTGTCCTCC AGTGACACAA TGGATTACCG ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( NM_001165979.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159451.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant 2, mRNA.

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CloneID	OHu27663
Clone ID Related Accession (Same CDS sequence)	NM_001165979.2
Accession Version	NM_001165979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX491865.1, AK289852.1, AF170071.1 and BC151854.1. On Jan 9, 2014 this sequence version replaced NM_001165979.1. Transcript Variant: This variant (2) lacks two exons and has an additional novel exon at its 5' end, compared to variant 1. These differences produce a unique 5' UTR and cause translation initiation at a novel start codon, compared to variant 1. The encoded protein (isoform 2; also known as PLCepsilon1b) has a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.240886.1, SRR1803616.145701.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTTCAG AAGGAAGTGC AGCAGGAAGA GACTTTGCAG GAATGGAAGA GGTGAGGCAG 
CTCCACGTGA GATTCTGCAA AGGGATTAAG ATTTGGCACC AGGCTTGGTT TCTGTGCAGC 
CTCTTAGGCA GAGAGCCCCA GGAGAGGGAA GCAGGCTGTC AGCTGTGGCT TTGCACGCTC 
TCAGCTGTCT TGAAGGTTGG TTGGCTCTTC CCGCTCTCTG AGGTACCCAA TTTTACCTTG 
TTAAAGGATG GATGTGGATG CTGGAGGCTT AAAGAAGACC AGATCTACAA TGCAGTGAGA 
AGAGAAGAAA CAGAAAATAC AGTTGGATCT CTACTCCATT TCCTCACCAA GCTCCCAGCC 
TCCGAGACAG CCCATGGAAG GATAAGCGTT GGTCCATGCT TAAAGCAATG TGTCCGAGAC 
ACTGTATGTG AGTATCGCGC CACCCTCCAA AGGACTTCAA TATCGCAGTA CATCACCGGT 
TCTCTCCTAG AAGCAACCAC GTCTTTGGGA GCAAGAAGTG GCCTTCTCAG TACTTTTGGA 
GGATCCACTG GACGAATGAT GCTGAAAGAA CGCCAGCCAG GCCCCTCTGT GGCCAATTCC 
AATGCCCTCC CTTCAAGTTC AGCTGGGATC AGCAAGGAGC TGATCGATCT GCAGCCTCTC 
ATCCAGTTCC CAGAGGAAGT CGCCAGCATC CTGATGGAGC AAGAGCAGAC TATTTACCGC 
AGGGTCTTGC CAGTCGACTA CCTTTGCTTC TTAACACGGG ACTTGGGCAC TCCTGAATGC 
CAGAGCTCCT TGCCCTGCCT CAAAGCATCC ATCTCAGCGT CGATTCTTAC CACTCAGAAT 
GGAGAGCACA ATGCCCTTGA AGATCTGGTG ATGAGGTTTA ATGAGGTGAG CTCCTGGGTG 
ACATGGCTGA TCCTCACGGC AGGCTCCATG GAGGAGAAGC GAGAAGTCTT TTCATATTTG 
GTGCATGTGG CCAAATGCTG CTGGAACATG GGCAACTACA ACGCTGTCAT GGAGTTCTTG 
GCTGGCCTCA GGTCAAGAAA AGTTTTAAAA ATGTGGCAGT TCATGGACCA GTCTGATATT 
GAGACCATGA GGAGCCTGAA GGATGCTATG GCCCAGCATG AGTCCTCTTG TGAGTACAGA 
AAGGTGGTGA CACGTGCCCT GCACATCCCT GGCTGTAAGG TGGTTCCATT CTGTGGGGTG 
TTTCTGAAGG AGCTCTGTGA AGTGCTTGAC GGCGCCTCCG GTCTCATGAA GCTTTGCCCG 
CGGTACAATT CCCAAGAAGA AACTTTAGAG TTTGTAGCAG ATTACAGTGG ACAAGATAAT 
TTCTTACAAC GAGTGGGACA AAATGGCTTA AAGAATTCGG AGAAGGAGTC CACTGTCAAC 
AGCATCTTTC AGGTCATCCG GAGCTGCAAT CGAAGTCTGG AGACAGACGA GGAGGACAGC 
CCCAGTGAAG GAAACAGCTC CAGGAAAAGC TCCTTGAAGG ATAAAAGCCG ATGGCAGTTT 
ATAATTGGAG ATTTGTTGGA TTCAGACAAT GACATCTTTG AGCAATCCAA AGAATACGAC 
TCTCATGGTT CAGAGGACTC ACAGAAGGCC TTCGACCATG GGACGGAGCT CATCCCTTGG 
TACGTGCTGT CCATCCAAGC CGATGTGCAC CAGTTCCTGC TGCAGGGGGC CACGGTCATC 
CACTACGACC AGGACACACA CCTCTCTGCC CGCTGCTTCC TCCAGCTTCA GCCCGACAAT 
AGCACCTTGA CCTGGGTAAA GCCCACAACT GCCTCCCCAG CCAGCAGTAA AGCAAAACTT 
GGTGTACTTA ATAACACAGC TGAGCCTGGA AAATTCCCAC TACTGGGTAA TGCTGGATTA 
AGTAGCCTGA CGGAAGGGGT CTTGGATCTT TTTGCAGTGA AGGCTGTATA CATGGGCCAC 
CCTGGCATTG ATATACACAC TGTGTGTGTT CAGAACAAAC TGGGTAGCAT GTTCCTGTCA 
GAGACTGGTG TGACATTGCT CTATGGGCTT CAGACCACAG ACAACAGATT ATTGCACTTC 
GTGGCACCAA AGCACACAGC TAAAATGCTC TTCAGCGGAT TATTGGAACT CACTAGAGCT 
GTGAGAAAGA TGAGGAAATT CCCTGACCAA AGACAGCAGT GGCTGCGGAA ACAGTACGTC 
AGCCTTTATC AGGAGGATGG ACGGTATGAA GGCCCAACTT TGGCTCACGC TGTGGAGTTG 
TTTGGTGGCA GACGGTGGAG TGCTCGAAAC CCCAGCCCCG GAACATCAGC AAAGAATGCT 
GAGAAGCCCA ATATGCAGAG AAACAATACC CTGGGCATAA GCACTACCAA GAAAAAGAAG 
AAAATCCTCA TGAGGGGTGA GAGTGGAGAG GTAACTGACG ATGAGATGGC AACCCGAAAG 
GCCAAGATGC ACAAAGAGTG TCGAAGCCGG AGTGGTTCTG ATCCTCAAGA CATTAATGAA 
CAAGAAGAAT CAGAGGTGAA TGCCATCGCT AACCCTCCAA ACCCCCTCCC TTCCAGAAGA 
GCCCACTCTT TGACCACAGC TGGGTCCCCC AACTTGGCTG CCGGGACGTC ATCTCCCATC 
AGGCCAGTGT CCTCCCCTGT GCTGTCTTCT TCAAACAAGA GCCCATCCAG TGCTTGGAGC 
AGTAGTAGCT GGCACGGGCG GATCAAAGGC GGCATGAAGG GATTTCAGAG CTTCATGGTT 
TCAGATAGCA ACATGAGTTT TGTTGAATTT GTTGAGCTGT TCAAATCATT CAGTGTCAGG 
AGCCGCAAGG ACCTGAAGGA TCTGTTTGAT GTCTATGCAG TGCCCTGCAA CCGATCTGGC 
TCCGAGTCAG CCCCACTCTA CACCAACCTG ACAATTGATG AAAACACCAG CGATCTTCAG 
CCTGACCTAG ATCTGTTGAC CAGAAATGTC TCGGATTTGG GGTTGTTCAT TAAGAGTAAA 
CAGCAGCTAT CGGACAACCA GAGGCAGATA TCTGATGCCA TTGCTGCTGC AAGCATTGTG 
ACAAATGGCA CTGGGATTGA GAGCACATCT CTGGGCATTT TTGGGGTGGG CATACTTCAG 
CTCAACGATT TCCTCGTGAA TTGCCAAGGA GAACACTGCA CTTATGATGA AATCCTCAGC 
ATCATCCAGA AGTTCGAGCC TAGCATCAGT ATGTGTCATC AGGGACTAAT GTCATTTGAA 
GGGTTTGCCA GGTTTCTGAT GGATAAAGAA AATTTTGCCT CAAAAAATGA TGAGTCACAG 
GAGAACATTA AAGAACTGCA GCTACCCCTC TCATACTATT ACATCGAATC TTCGCACAAT 
ACCTACCTCA CGGGCCATCA GCTCAAAGGA GAATCCTCGG TAGAACTCTA CAGCCAGGTC 
CTTTTGCAAG GCTGTCGAAG TGTAGAATTG GACTGCTGGG ACGGAGACGA TGGGATGCCC 
ATCATTTATC ATGGACATAC GCTGACAACC AAGATCCCCT TCAAGGAAGT GGTTGAAGCC 
ATTGATCGCA GTGCCTTCAT CAACTCTGAC CTGCCAATCA TCATATCGAT TGAGAACCAC 
TGTTCATTGC CTCAGCAACG AAAAATGGCA GAAATTTTCA AGACTGTGTT TGGAGAAAAG 
CTGGTGACTA AATTCTTATT TGAGACTGAT TTCTCAGATG ATCCAATGCT TCCTTCACCT 
GACCAACTCA GAAAGAAAGT TCTTCTTAAA AACAAGAAGC TAAAAGCCCA TCAGACGCCA 
GTGGATATCT TAAAGCAAAA GGCTCATCAG TTAGCATCTA TGCAAGTGCA GGCTTATAAT 
GGTGGGAATG CCAACCCCCG ACCTGCCAAT AATGAGGAAG AGGAAGATGA GGAGGACGAA 
TATGATTATG ACTATGAATC CCTTTCTGAT GACAACATTC TGGAAGACAG ACCTGAAAAT 
AAATCATGTA ATGACAAGCT TCAGTTTGAA TATAATGAAG AAATCCCAAA GAGGATAAAG 
AAAGCAGATA ACTCTGCTTG CAACAAAGGA AAGGTTTATG ATATGGAACT GGGAGAAGAA 
TTTTATCTTG ATCAGAATAA AAAGGAAAGC AGACAGATTG CACCAGAGCT TTCTGACCTT 
GTAATCTATT GTCAAGCAGT AAAATTTCCA GGACTGTCAA CTCTAAATGC ATCTGGCTCT 
AGCAGAGGAA AAGAAAGGAA AAGCAGGAAG TCCATTTTTG GCAACAATCC GGGCAGAATG 
AGCCCAGGGG AGACAGCATC ATTTAACAAA ACATCTGGAA AAAGTTCCTG TGAAGGCATT 
CGACAGACCT GGGAGGAATC TTCTTCCCCT CTCAACCCAA CCACGTCCCT CAGTGCTATC 
ATTAGAACTC CCAAATGTTA TCATATCTCG TCGCTGAATG AAAATGCCGC CAAACGTCTG 
TGTCGCAGGT ATTCTCAGAA ACTGACCCAG CACACCGCCT GTCAGCTGCT GAGAACTTAC 
CCTGCTGCCA CCCGCATCGA CTCTTCCAAC CCGAACCCCC TCATGTTCTG GCTCCATGGG 
ATACAGCTTG TGGCACTCAA CTACCAGACT GATGATCTCC CTTTACATTT AAATGCTGCA 
ATGTTTGAGG CAAATGGTGG TTGTGGTTAT GTATTGAAAC CTCCAGTTCT GTGGGACAAG 
AACTGCCCCA TGTATCAGAA GTTTTCTCCA CTAGAAAGAG ATCTGGACAG CATGGATCCT 
GCAGTCTATT CTTTAACTAT TGTCTCTGGT CAGAATGTGT GCCCCAGTAA TAGCATGGGA 
AGCCCGTGCA TTGAAGTCGA CGTCCTGGGC ATGCCTCTGG ACAGCTGCCA TTTCCGCACA 
AAGCCCATCC ATCGAAACAC CCTGAACCCC ATGTGGAACG AGCAGTTTCT GTTCCACGTT 
CACTTCGAAG ATCTTGTATT TCTTCGTTTT GCAGTTGTGG AAAACAATAG TTCAGCGGTA 
ACTGCTCAGA GAATCATTCC ACTGAAAGCT TTAAAACGAG GATATCGACA TCTTCAGCTG 
CGAAACCTTC ACAATGAAGT CTTGGAGATT TCTAGTTTAT TCATTAACAG CAGAAGGATG 
GAAGAAAATT CCTCTGGCAA TACCATGTCA GCCTCTTCGA TGTTTAATAC AGAAGAAAGA 
AAATGTTTGC AGACTCACAG AGTCACGGTG CATGGGGTCC CAGGGCCAGA GCCCTTTACC 
GTTTTCACTA TTAATGGAGG CACCAAGGCA AAGCAGCTTC TGCAGCAAAT TCTGACAAAT 
GAACAAGACA TCAAACCTGT TACCACAGAC TATTTTTTGA TGGAAGAAAA ATATTTTATA 
TCTAAAGAAA AGAATGAATG TAGGAAACAA CCATTCCAGA GAGCCATTGG TCCAGAAGAG 
GAGATCATGC AAATTTTAAG CAGCTGGTTT CCAGAAGAGG GATACATGGG CAGGATTGTC 
TTAAAAACCC AGCAGGAAAA CCTAGAAGAG AAAAACATTG TTCAAGATGA CAAAGAGGTG 
ATCTTGAGCT CAGAGGAGGA GAGTTTCTTT GTCCAAGTGC ATGATGTTTC TCCAGAGCAA 
CCTCGAACAG TCATCAAAGC ACCCCGCGTC AGCACTGCAC AGGATGTCAT TCAGCAGACC 
TTATGCAAAG CCAAATATTC CTACAGCATC CTGAGCAACC CCAATCCAAG CGACTATGTG 
CTTTTGGAAG AGGTGGTGAA AGACACTACC AACAAGAAGA CTACCACACC AAAGTCCTCT 
CAGCGGGTCC TTCTGGATCA GGAGTGTGTG TTTCAAGCCC AAAGCAAGTG GAAAGGTGCA 
GGAAAATTCA TCCTTAAGCT AAAGGAGCAG GTGCAGGCAT CTCGAGAAGA TAAAAAGAAA 
GGCATTTCTT TCGCAAGTGA ACTCAAGAAG CTCACCAAGT CAACTAAACA GCCCCGAGGA 
CTTACATCAC CTTCTCAGCT CTTGACCTCA GAAAGTATCC AAACCAAGGA GGAGAAACCT 
GTGGGTGGCT TGTCCTCCAG TGACACAATG GATTACCGAC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159451.1
CDS	70..6054
Translation	MVSEGSAAGRDFAGMEEVRQLHVRFCKGIKIWHQAWFLCSLLGREPQEREAGCQLWLCTLSAVLKVGWLFPLSEVPNFTLLKDGCGCWRLKEDQIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	NM_001165979.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165979.2

ORF Insert Sequence:

ATGGTTTCAG AAGGAAGTGC AGCAGGAAGA GACTTTGCAG GAATGGAAGA GGTGAGGCAG 
CTCCACGTGA GATTCTGCAA AGGGATTAAG ATTTGGCACC AGGCTTGGTT TCTGTGCAGC 
CTCTTAGGCA GAGAGCCCCA GGAGAGGGAA GCAGGCTGTC AGCTGTGGCT TTGCACGCTC 
TCAGCTGTCT TGAAGGTTGG TTGGCTCTTC CCGCTCTCTG AGGTACCCAA TTTTACCTTG 
TTAAAGGATG GATGTGGATG CTGGAGGCTT AAAGAAGACC AGATCTACAA TGCAGTGAGA 
AGAGAAGAAA CAGAAAATAC AGTTGGATCT CTACTCCATT TCCTCACCAA GCTCCCAGCC 
TCCGAGACAG CCCATGGAAG GATAAGCGTT GGTCCATGCT TAAAGCAATG TGTCCGAGAC 
ACTGTATGTG AGTATCGCGC CACCCTCCAA AGGACTTCAA TATCGCAGTA CATCACCGGT 
TCTCTCCTAG AAGCAACCAC GTCTTTGGGA GCAAGAAGTG GCCTTCTCAG TACTTTTGGA 
GGATCCACTG GACGAATGAT GCTGAAAGAA CGCCAGCCAG GCCCCTCTGT GGCCAATTCC 
AATGCCCTCC CTTCAAGTTC AGCTGGGATC AGCAAGGAGC TGATCGATCT GCAGCCTCTC 
ATCCAGTTCC CAGAGGAAGT CGCCAGCATC CTGATGGAGC AAGAGCAGAC TATTTACCGC 
AGGGTCTTGC CAGTCGACTA CCTTTGCTTC TTAACACGGG ACTTGGGCAC TCCTGAATGC 
CAGAGCTCCT TGCCCTGCCT CAAAGCATCC ATCTCAGCGT CGATTCTTAC CACTCAGAAT 
GGAGAGCACA ATGCCCTTGA AGATCTGGTG ATGAGGTTTA ATGAGGTGAG CTCCTGGGTG 
ACATGGCTGA TCCTCACGGC AGGCTCCATG GAGGAGAAGC GAGAAGTCTT TTCATATTTG 
GTGCATGTGG CCAAATGCTG CTGGAACATG GGCAACTACA ACGCTGTCAT GGAGTTCTTG 
GCTGGCCTCA GGTCAAGAAA AGTTTTAAAA ATGTGGCAGT TCATGGACCA GTCTGATATT 
GAGACCATGA GGAGCCTGAA GGATGCTATG GCCCAGCATG AGTCCTCTTG TGAGTACAGA 
AAGGTGGTGA CACGTGCCCT GCACATCCCT GGCTGTAAGG TGGTTCCATT CTGTGGGGTG 
TTTCTGAAGG AGCTCTGTGA AGTGCTTGAC GGCGCCTCCG GTCTCATGAA GCTTTGCCCG 
CGGTACAATT CCCAAGAAGA AACTTTAGAG TTTGTAGCAG ATTACAGTGG ACAAGATAAT 
TTCTTACAAC GAGTGGGACA AAATGGCTTA AAGAATTCGG AGAAGGAGTC CACTGTCAAC 
AGCATCTTTC AGGTCATCCG GAGCTGCAAT CGAAGTCTGG AGACAGACGA GGAGGACAGC 
CCCAGTGAAG GAAACAGCTC CAGGAAAAGC TCCTTGAAGG ATAAAAGCCG ATGGCAGTTT 
ATAATTGGAG ATTTGTTGGA TTCAGACAAT GACATCTTTG AGCAATCCAA AGAATACGAC 
TCTCATGGTT CAGAGGACTC ACAGAAGGCC TTCGACCATG GGACGGAGCT CATCCCTTGG 
TACGTGCTGT CCATCCAAGC CGATGTGCAC CAGTTCCTGC TGCAGGGGGC CACGGTCATC 
CACTACGACC AGGACACACA CCTCTCTGCC CGCTGCTTCC TCCAGCTTCA GCCCGACAAT 
AGCACCTTGA CCTGGGTAAA GCCCACAACT GCCTCCCCAG CCAGCAGTAA AGCAAAACTT 
GGTGTACTTA ATAACACAGC TGAGCCTGGA AAATTCCCAC TACTGGGTAA TGCTGGATTA 
AGTAGCCTGA CGGAAGGGGT CTTGGATCTT TTTGCAGTGA AGGCTGTATA CATGGGCCAC 
CCTGGCATTG ATATACACAC TGTGTGTGTT CAGAACAAAC TGGGTAGCAT GTTCCTGTCA 
GAGACTGGTG TGACATTGCT CTATGGGCTT CAGACCACAG ACAACAGATT ATTGCACTTC 
GTGGCACCAA AGCACACAGC TAAAATGCTC TTCAGCGGAT TATTGGAACT CACTAGAGCT 
GTGAGAAAGA TGAGGAAATT CCCTGACCAA AGACAGCAGT GGCTGCGGAA ACAGTACGTC 
AGCCTTTATC AGGAGGATGG ACGGTATGAA GGCCCAACTT TGGCTCACGC TGTGGAGTTG 
TTTGGTGGCA GACGGTGGAG TGCTCGAAAC CCCAGCCCCG GAACATCAGC AAAGAATGCT 
GAGAAGCCCA ATATGCAGAG AAACAATACC CTGGGCATAA GCACTACCAA GAAAAAGAAG 
AAAATCCTCA TGAGGGGTGA GAGTGGAGAG GTAACTGACG ATGAGATGGC AACCCGAAAG 
GCCAAGATGC ACAAAGAGTG TCGAAGCCGG AGTGGTTCTG ATCCTCAAGA CATTAATGAA 
CAAGAAGAAT CAGAGGTGAA TGCCATCGCT AACCCTCCAA ACCCCCTCCC TTCCAGAAGA 
GCCCACTCTT TGACCACAGC TGGGTCCCCC AACTTGGCTG CCGGGACGTC ATCTCCCATC 
AGGCCAGTGT CCTCCCCTGT GCTGTCTTCT TCAAACAAGA GCCCATCCAG TGCTTGGAGC 
AGTAGTAGCT GGCACGGGCG GATCAAAGGC GGCATGAAGG GATTTCAGAG CTTCATGGTT 
TCAGATAGCA ACATGAGTTT TGTTGAATTT GTTGAGCTGT TCAAATCATT CAGTGTCAGG 
AGCCGCAAGG ACCTGAAGGA TCTGTTTGAT GTCTATGCAG TGCCCTGCAA CCGATCTGGC 
TCCGAGTCAG CCCCACTCTA CACCAACCTG ACAATTGATG AAAACACCAG CGATCTTCAG 
CCTGACCTAG ATCTGTTGAC CAGAAATGTC TCGGATTTGG GGTTGTTCAT TAAGAGTAAA 
CAGCAGCTAT CGGACAACCA GAGGCAGATA TCTGATGCCA TTGCTGCTGC AAGCATTGTG 
ACAAATGGCA CTGGGATTGA GAGCACATCT CTGGGCATTT TTGGGGTGGG CATACTTCAG 
CTCAACGATT TCCTCGTGAA TTGCCAAGGA GAACACTGCA CTTATGATGA AATCCTCAGC 
ATCATCCAGA AGTTCGAGCC TAGCATCAGT ATGTGTCATC AGGGACTAAT GTCATTTGAA 
GGGTTTGCCA GGTTTCTGAT GGATAAAGAA AATTTTGCCT CAAAAAATGA TGAGTCACAG 
GAGAACATTA AAGAACTGCA GCTACCCCTC TCATACTATT ACATCGAATC TTCGCACAAT 
ACCTACCTCA CGGGCCATCA GCTCAAAGGA GAATCCTCGG TAGAACTCTA CAGCCAGGTC 
CTTTTGCAAG GCTGTCGAAG TGTAGAATTG GACTGCTGGG ACGGAGACGA TGGGATGCCC 
ATCATTTATC ATGGACATAC GCTGACAACC AAGATCCCCT TCAAGGAAGT GGTTGAAGCC 
ATTGATCGCA GTGCCTTCAT CAACTCTGAC CTGCCAATCA TCATATCGAT TGAGAACCAC 
TGTTCATTGC CTCAGCAACG AAAAATGGCA GAAATTTTCA AGACTGTGTT TGGAGAAAAG 
CTGGTGACTA AATTCTTATT TGAGACTGAT TTCTCAGATG ATCCAATGCT TCCTTCACCT 
GACCAACTCA GAAAGAAAGT TCTTCTTAAA AACAAGAAGC TAAAAGCCCA TCAGACGCCA 
GTGGATATCT TAAAGCAAAA GGCTCATCAG TTAGCATCTA TGCAAGTGCA GGCTTATAAT 
GGTGGGAATG CCAACCCCCG ACCTGCCAAT AATGAGGAAG AGGAAGATGA GGAGGACGAA 
TATGATTATG ACTATGAATC CCTTTCTGAT GACAACATTC TGGAAGACAG ACCTGAAAAT 
AAATCATGTA ATGACAAGCT TCAGTTTGAA TATAATGAAG AAATCCCAAA GAGGATAAAG 
AAAGCAGATA ACTCTGCTTG CAACAAAGGA AAGGTTTATG ATATGGAACT GGGAGAAGAA 
TTTTATCTTG ATCAGAATAA AAAGGAAAGC AGACAGATTG CACCAGAGCT TTCTGACCTT 
GTAATCTATT GTCAAGCAGT AAAATTTCCA GGACTGTCAA CTCTAAATGC ATCTGGCTCT 
AGCAGAGGAA AAGAAAGGAA AAGCAGGAAG TCCATTTTTG GCAACAATCC GGGCAGAATG 
AGCCCAGGGG AGACAGCATC ATTTAACAAA ACATCTGGAA AAAGTTCCTG TGAAGGCATT 
CGACAGACCT GGGAGGAATC TTCTTCCCCT CTCAACCCAA CCACGTCCCT CAGTGCTATC 
ATTAGAACTC CCAAATGTTA TCATATCTCG TCGCTGAATG AAAATGCCGC CAAACGTCTG 
TGTCGCAGGT ATTCTCAGAA ACTGACCCAG CACACCGCCT GTCAGCTGCT GAGAACTTAC 
CCTGCTGCCA CCCGCATCGA CTCTTCCAAC CCGAACCCCC TCATGTTCTG GCTCCATGGG 
ATACAGCTTG TGGCACTCAA CTACCAGACT GATGATCTCC CTTTACATTT AAATGCTGCA 
ATGTTTGAGG CAAATGGTGG TTGTGGTTAT GTATTGAAAC CTCCAGTTCT GTGGGACAAG 
AACTGCCCCA TGTATCAGAA GTTTTCTCCA CTAGAAAGAG ATCTGGACAG CATGGATCCT 
GCAGTCTATT CTTTAACTAT TGTCTCTGGT CAGAATGTGT GCCCCAGTAA TAGCATGGGA 
AGCCCGTGCA TTGAAGTCGA CGTCCTGGGC ATGCCTCTGG ACAGCTGCCA TTTCCGCACA 
AAGCCCATCC ATCGAAACAC CCTGAACCCC ATGTGGAACG AGCAGTTTCT GTTCCACGTT 
CACTTCGAAG ATCTTGTATT TCTTCGTTTT GCAGTTGTGG AAAACAATAG TTCAGCGGTA 
ACTGCTCAGA GAATCATTCC ACTGAAAGCT TTAAAACGAG GATATCGACA TCTTCAGCTG 
CGAAACCTTC ACAATGAAGT CTTGGAGATT TCTAGTTTAT TCATTAACAG CAGAAGGATG 
GAAGAAAATT CCTCTGGCAA TACCATGTCA GCCTCTTCGA TGTTTAATAC AGAAGAAAGA 
AAATGTTTGC AGACTCACAG AGTCACGGTG CATGGGGTCC CAGGGCCAGA GCCCTTTACC 
GTTTTCACTA TTAATGGAGG CACCAAGGCA AAGCAGCTTC TGCAGCAAAT TCTGACAAAT 
GAACAAGACA TCAAACCTGT TACCACAGAC TATTTTTTGA TGGAAGAAAA ATATTTTATA 
TCTAAAGAAA AGAATGAATG TAGGAAACAA CCATTCCAGA GAGCCATTGG TCCAGAAGAG 
GAGATCATGC AAATTTTAAG CAGCTGGTTT CCAGAAGAGG GATACATGGG CAGGATTGTC 
TTAAAAACCC AGCAGGAAAA CCTAGAAGAG AAAAACATTG TTCAAGATGA CAAAGAGGTG 
ATCTTGAGCT CAGAGGAGGA GAGTTTCTTT GTCCAAGTGC ATGATGTTTC TCCAGAGCAA 
CCTCGAACAG TCATCAAAGC ACCCCGCGTC AGCACTGCAC AGGATGTCAT TCAGCAGACC 
TTATGCAAAG CCAAATATTC CTACAGCATC CTGAGCAACC CCAATCCAAG CGACTATGTG 
CTTTTGGAAG AGGTGGTGAA AGACACTACC AACAAGAAGA CTACCACACC AAAGTCCTCT 
CAGCGGGTCC TTCTGGATCA GGAGTGTGTG TTTCAAGCCC AAAGCAAGTG GAAAGGTGCA 
GGAAAATTCA TCCTTAAGCT AAAGGAGCAG GTGCAGGCAT CTCGAGAAGA TAAAAAGAAA 
GGCATTTCTT TCGCAAGTGA ACTCAAGAAG CTCACCAAGT CAACTAAACA GCCCCGAGGA 
CTTACATCAC CTTCTCAGCT CTTGACCTCA GAAAGTATCC AAACCAAGGA GGAGAAACCT 
GTGGGTGGCT TGTCCTCCAG TGACACAATG GATTACCGAC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_011539850.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538152.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X9, mRNA.

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CloneID	OHu67755
Clone ID Related Accession (Same CDS sequence)	XM_011539850.3 , XM_011539850.2
Accession Version	XM_011539850.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011539850.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCAG AAGGAAGTGC AGCAGGAAGA GACTTTGCAG GAATGGAAGA GATCTACAAT 
GCAGTGAGAA GAGAAGAAAC AGAAAATACA GTTGGATCTC TACTCCATTT CCTCACCAAG 
CTCCCAGCCT CCGAGACAGC CCATGGAAGG ATAAGCGTTG GTCCATGCTT AAAGCAATGT 
GTCCGAGACA CTGTATGTGA GTATCGCGCC ACCCTCCAAA GGACTTCAAT ATCGCAGTAC 
ATCACCGGTT CTCTCCTAGA AGCAACCACG TCTTTGGGAG CAAGAAGTGG CCTTCTCAGT 
ACTTTTGGAG GATCCACTGG ACGAATGATG CTGAAAGAAC GCCAGCCAGG CCCCTCTGTG 
GCCAATTCCA ATGCCCTCCC TTCAAGTTCA GCTGGGATCA GCAAGGAGCT GATCGATCTG 
CAGCCTCTCA TCCAGTTCCC AGAGGAAGTC GCCAGCATCC TGATGGAGCA AGAGCAGACT 
ATTTACCGCA GGGTCTTGCC AGTCGACTAC CTTTGCTTCT TAACACGGGA CTTGGGCACT 
CCTGAATGCC AGAGCTCCTT GCCCTGCCTC AAAGCATCCA TCTCAGCGTC GATTCTTACC 
ACTCAGAATG GAGAGCACAA TGCCCTTGAA GATCTGGTGA TGAGGTTTAA TGAGGTGAGC 
TCCTGGGTGA CATGGCTGAT CCTCACGGCA GGCTCCATGG AGGAGAAGCG AGAAGTCTTT 
TCATATTTGG TGCATGTGGC CAAATGCTGC TGGAACATGG GCAACTACAA CGCTGTCATG 
GAGTTCTTGG CTGGCCTCAG GTCAAGAAAA GTTTTAAAAA TGTGGCAGTT CATGGACCAG 
TCTGATATTG AGACCATGAG GAGCCTGAAG GATGCTATGG CCCAGCATGA GTCCTCTTGT 
GAGTACAGAA AGGTGGTGAC ACGTGCCCTG CACATCCCTG GCTGTAAGGT GGTTCCATTC 
TGTGGGGTGT TTCTGAAGGA GCTCTGTGAA GTGCTTGACG GCGCCTCCGG TCTCATGAAG 
CTTTGCCCGC GGTACAATTC CCAAGAAGAA ACTTTAGAGT TTGTAGCAGA TTACAGTGGA 
CAAGATAATT TCTTACAACG AGTGGGACAA AATGGCTTAA AGAATTCGGA GAAGGAGTCC 
ACTGTCAACA GCATCTTTCA GGTCATCCGG AGCTGCAATC GAAGTCTGGA GACAGACGAG 
GAGGACAGCC CCAGTGAAGG AAACAGCTCC AGGAAAAGCT CCTTGAAGGA TAAAAGCCGA 
TGGCAGTTTA TAATTGGAGA TTTGTTGGAT TCAGACAATG ACATCTTTGA GCAATCCAAA 
GAATACGACT CTCATGGTTC AGAGGACTCA CAGAAGGCCT TCGACCATGG GACGGAGCTC 
ATCCCTTGGT ACGTGCTGTC CATCCAAGCC GATGTGCACC AGTTCCTGCT GCAGGGGGCC 
ACGGTCATCC ACTACGACCA GGACACACAC CTCTCTGCCC GCTGCTTCCT CCAGCTTCAG 
CCCGACAATA GCACCTTGAC CTGGGTAAAG CCCACAACTG CCTCCCCAGC CAGCAGTAAA 
GCAAAACTTG GTGTACTTAA TAACACAGCT GAGCCTGGAA AATTCCCACT ACTGGGTAAT 
GCTGGATTAA GTAGCCTGAC GGAAGGGGTC TTGGATCTTT TTGCAGTGAA GGCTGTATAC 
ATGGGCCACC CTGGCATTGA TATACACACT GTGTGTGTTC AGAACAAACT GGGTAGCATG 
TTCCTGTCAG AGACTGGTGT GACATTGCTC TATGGGCTTC AGACCACAGA CAACAGATTA 
TTGCACTTCG TGGCACCAAA GCACACAGCT AAAATGCTCT TCAGCGGATT ATTGGAACTC 
ACTAGAGCTG TGAGAAAGAT GAGGAAATTC CCTGACCAAA GACAGCAGTG GCTGCGGAAA 
CAGTACGTCA GCCTTTATCA GGAGGATGGA CGGTATGAAG GCCCAACTTT GGCTCACGCT 
GTGGAGTTGT TTGGTGGCAG ACGGTGGAGT GCTCGAAACC CCAGCCCCGG AACATCAGCA 
AAGAATGCTG AGAAGCCCAA TATGCAGAGA AACAATACCC TGGGCATAAG CACTACCAAG 
AAAAAGAAGA AAATCCTCAT GAGGGGTGAG AGTGGAGAGG TAACTGACGA TGAGATGGCA 
ACCCGAAAGG CCAAGATGCA CAAAGAGTGT CGAAGCCGGA GTGGTTCTGA TCCTCAAGAC 
ATTAATGAAC AAGAAGAATC AGAGGTGAAT GCCATCGCTA ACCCTCCAAA CCCCCTCCCT 
TCCAGAAGAG CCCACTCTTT GACCACAGCT GGGTCCCCCA ACTTGGCTGC CGGGACGTCA 
TCTCCCATCA GGCCAGTGTC CTCCCCTGTG CTGTCTTCTT CAAACAAGAG CCCATCCAGT 
GCTTGGAGCA GTAGTAGCTG GCACGGGCGG ATCAAAGGCG GCATGAAGGG ATTTCAGAGC 
TTCATGGTTT CAGATAGCAA CATGAGTTTT GTTGAATTTG TTGAGCTGTT CAAATCATTC 
AGTGTCAGGA GCCGCAAGGA CCTGAAGGAT CTGTTTGATG TCTATGCAGT GCCCTGCAAC 
CGATCTGGCT CCGAGTCAGC CCCACTCTAC ACCAACCTGA CAATTGATGA AAACACCAGC 
GATCTTCAGC CTGACCTAGA TCTGTTGACC AGAAATGTCT CGGATTTGGG GTTGTTCATT 
AAGAGTAAAC AGCAGCTATC GGACAACCAG AGGCAGATAT CTGATGCCAT TGCTGCTGCA 
AGCATTGTGA CAAATGGCAC TGGGATTGAG AGCACATCTC TGGGCATTTT TGGGGTGGGC 
ATACTTCAGC TCAACGATTT CCTCGTGAAT TGCCAAGGAG AACACTGCAC TTATGATGAA 
ATCCTCAGCA TCATCCAGAA GTTCGAGCCT AGCATCAGTA TGTGTCATCA GGGACTAATG 
TCATTTGAAG GGTTTGCCAG GTTTCTGATG GATAAAGAAA ATTTTGCCTC AAAAAATGAT 
GAGTCACAGG AGAACATTAA AGAACTGCAG CTACCCCTCT CATACTATTA CATCGAATCT 
TCGCACAATA CCTACCTCAC GGGCCATCAG CTCAAAGGAG AATCCTCGGT AGAACTCTAC 
AGCCAGGTCC TTTTGCAAGG CTGTCGAAGT GTAGAATTGG ACTGCTGGGA CGGAGACGAT 
GGGATGCCCA TCATTTATCA TGGACATACG CTGACAACCA AGATCCCCTT CAAGGAAGTG 
GTTGAAGCCA TTGATCGCAG TGCCTTCATC AACTCTGACC TGCCAATCAT CATATCGATT 
GAGAACCACT GTTCATTGCC TCAGCAACGA AAAATGGCAG AAATTTTCAA GACTGTGTTT 
GGAGAAAAGC TGGTGACTAA ATTCTTATTT GAGACTGATT TCTCAGATGA TCCAATGCTT 
CCTTCACCTG ACCAACTCAG AAAGAAAGTT CTTCTTAAAA ACAAGAAGCT AAAAGCCCAT 
CAGACGCCAG TGGATATCTT AAAGCAAAAG GCTCATCAGT TAGCATCTAT GCAAGTGCAG 
GCTTATAATG GTGGGAATGC CAACCCCCGA CCTGCCAATA ATGAGGAAGA GGAAGATGAG 
GAGGACGAAT ATGATTATGA CTATGAATCC CTTTCTGATG CAGATGTCCT TACTGCCTCC 
CCTGCTCCTA ACGGTCAGGA AGACAACATT CTGGAAGACA GACCTGAAAA TAAATCATGT 
AATGACAAGC TTCAGTTTGA ATATAATGAA GAAATCCCAA AGAGGATAAA GAAAGCAGAT 
AACTCTGCTT GCAACAAAGG AAAGGTTTAT GATATGGAAC TGGGAGAAGA ATTTTATCTT 
GATCAGAATA AAAAGGAAAG CAGACAGATT GCACCAGAGC TTTCTGACCT TGTAATCTAT 
TGTCAAGCAG TAAAATTTCC AGGACTGTCA ACTCTAAATG CATCTGGCTC TAGCAGAGGA 
AAAGAAAGGA AAAGCAGGAA GTCCATTTTT GGCAACAATC CGGGCAGAAT GAGCCCAGGG 
GAGACAGCAT CATTTAACAA AACATCTGGA AAAAGTTCCT GTGAAGGCAT TCGACAGACC 
TGGGAGGAAT CTTCTTCCCC TCTCAACCCA ACCACGTCCC TCAGTGCTAT CATTAGAACT 
CCCAAATGTT ATCATATCTC GTCGCTGAAT GAAAATGCCG CCAAACGTCT GTGTCGCAGG 
TATTCTCAGA AACTGACCCA GCACACCGCC TGTCAGCTGC TGAGAACTTA CCCTGCTGCC 
ACCCGCATCG ACTCTTCCAA CCCGAACCCC CTCATGTTCT GGCTCCATGG GATACAGCTT 
GTGGCACTCA ACTACCAGAC TGATGATCTC CCTTTACATT TAAATGCTGC AATGTTTGAG 
GCAAATGGTG GTTGTGGTTA TGTATTGAAA CCTCCAGTTC TGTGGGACAA GAACTGCCCC 
ATGTATCAGA AGTTTTCTCC ACTAGAAAGA GATCTGGACA GCATGGATCC TGCAGTCTAT 
TCTTTAACTA TTGTCTCTGG TCAGAATGTG TGCCCCAGTA ATAGCATGGG AAGCCCGTGC 
ATTGAAGTCG ACGTCCTGGG CATGCCTCTG GACAGCTGCC ATTTCCGCAC AAAGCCCATC 
CATCGAAACA CCCTGAACCC CATGTGGAAC GAGCAGTTTC TGTTCCACGT TCACTTCGAA 
GATCTTGTAT TTCTTCGTTT TGCAGTTGTG GAAAACAATA GTTCAGCGGT AACTGCTCAG 
AGAATCATTC CACTGAAAGC TTTAAAACGA GGATATCGAC ATCTTCAGCT GCGAAACCTT 
CACAATGAAG TCTTGGAGAT TTCTAGTTTA TTCATTAACA GCAGAAGGAT GGAAGAAAAT 
TCCTCTGGCA ATACCATGTC AGCCTCTTCG ATGTTTAATA CAGAAGAAAG AAAATGTTTG 
CAGACTCACA GAGTCACGGT GCATGGGGTC CCAGGGCCAG AGCCCTTTAC CGTTTTCACT 
ATTAATGGAG GCACCAAGGC AAAGCAGCTT CTGCAGCAAA TTCTGACAAA TGAACAAGAC 
ATCAAACCTG TTACCACAGA CTATTTTTTG ATGGAAGAAA AATATTTTAT ATCTAAAGAA 
AAGAATGAAT GTAGGAAACA ACCATTCCAG AGAGCCATTG GTCCAGAAGA GGAGATCATG 
CAAATTTTAA GCAGCTGGTT TCCAGAAGAG GGATACATGG GCAGGATTGT CTTAAAAACC 
CAGCAGGAAA ACCTAGAAGA GAAAAACATT GTTCAAGATG ACAAAGAGGT GATCTTGAGC 
TCAGAGGAGG AGAGTTTCTT TGTCCAAGTG CATGATGTTT CTCCAGAGCA ACCTCGAACA 
GTCATCAAAG CACCCCGCGT CAGCACTGCA CAGGATGTCA TTCAGCAGAC CTTATGCAAA 
GCCAAATATT CCTACAGCAT CCTGAGCAAC CCCAATCCAA GCGACTATGT GCTTTTGGAA 
GAGGTGGTGA AAGACACTAC CAACAAGAAG ACTACCACAC CAAAGTCCTC TCAGCGGGTC 
CTTCTGGATC AGGAGTGTGT GTTTCAAGCC CAAAGCAAGT GGAAAGGTGC AGGAAAATTC 
ATCCTTAAGC TAAAGGAGCA GGTGCAGGCA TCTCGAGAAG ATAAAAAGAA AGGCATTTCT 
TTCGCAAGTG AACTCAAGAA GCTCACCAAG TCAACTAAAC AGCCCCGAGG ACTTACATCA 
CCTTCTCAGC TCTTGACCTC AGAAAGTATC CAAACCAAGG AGGAGAAACC TGTGGGTGGC 
TTGTCCTCCA GTGACACAAT GGATTACCGA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538152.1
CDS	1383..7178
Translation	MVSEGSAAGRDFAGMEEIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDADVLTASPAPNGQEDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_011539850.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539850.3

ORF Insert Sequence:

ATGGTTTCAG AAGGAAGTGC AGCAGGAAGA GACTTTGCAG GAATGGAAGA GATCTACAAT 
GCAGTGAGAA GAGAAGAAAC AGAAAATACA GTTGGATCTC TACTCCATTT CCTCACCAAG 
CTCCCAGCCT CCGAGACAGC CCATGGAAGG ATAAGCGTTG GTCCATGCTT AAAGCAATGT 
GTCCGAGACA CTGTATGTGA GTATCGCGCC ACCCTCCAAA GGACTTCAAT ATCGCAGTAC 
ATCACCGGTT CTCTCCTAGA AGCAACCACG TCTTTGGGAG CAAGAAGTGG CCTTCTCAGT 
ACTTTTGGAG GATCCACTGG ACGAATGATG CTGAAAGAAC GCCAGCCAGG CCCCTCTGTG 
GCCAATTCCA ATGCCCTCCC TTCAAGTTCA GCTGGGATCA GCAAGGAGCT GATCGATCTG 
CAGCCTCTCA TCCAGTTCCC AGAGGAAGTC GCCAGCATCC TGATGGAGCA AGAGCAGACT 
ATTTACCGCA GGGTCTTGCC AGTCGACTAC CTTTGCTTCT TAACACGGGA CTTGGGCACT 
CCTGAATGCC AGAGCTCCTT GCCCTGCCTC AAAGCATCCA TCTCAGCGTC GATTCTTACC 
ACTCAGAATG GAGAGCACAA TGCCCTTGAA GATCTGGTGA TGAGGTTTAA TGAGGTGAGC 
TCCTGGGTGA CATGGCTGAT CCTCACGGCA GGCTCCATGG AGGAGAAGCG AGAAGTCTTT 
TCATATTTGG TGCATGTGGC CAAATGCTGC TGGAACATGG GCAACTACAA CGCTGTCATG 
GAGTTCTTGG CTGGCCTCAG GTCAAGAAAA GTTTTAAAAA TGTGGCAGTT CATGGACCAG 
TCTGATATTG AGACCATGAG GAGCCTGAAG GATGCTATGG CCCAGCATGA GTCCTCTTGT 
GAGTACAGAA AGGTGGTGAC ACGTGCCCTG CACATCCCTG GCTGTAAGGT GGTTCCATTC 
TGTGGGGTGT TTCTGAAGGA GCTCTGTGAA GTGCTTGACG GCGCCTCCGG TCTCATGAAG 
CTTTGCCCGC GGTACAATTC CCAAGAAGAA ACTTTAGAGT TTGTAGCAGA TTACAGTGGA 
CAAGATAATT TCTTACAACG AGTGGGACAA AATGGCTTAA AGAATTCGGA GAAGGAGTCC 
ACTGTCAACA GCATCTTTCA GGTCATCCGG AGCTGCAATC GAAGTCTGGA GACAGACGAG 
GAGGACAGCC CCAGTGAAGG AAACAGCTCC AGGAAAAGCT CCTTGAAGGA TAAAAGCCGA 
TGGCAGTTTA TAATTGGAGA TTTGTTGGAT TCAGACAATG ACATCTTTGA GCAATCCAAA 
GAATACGACT CTCATGGTTC AGAGGACTCA CAGAAGGCCT TCGACCATGG GACGGAGCTC 
ATCCCTTGGT ACGTGCTGTC CATCCAAGCC GATGTGCACC AGTTCCTGCT GCAGGGGGCC 
ACGGTCATCC ACTACGACCA GGACACACAC CTCTCTGCCC GCTGCTTCCT CCAGCTTCAG 
CCCGACAATA GCACCTTGAC CTGGGTAAAG CCCACAACTG CCTCCCCAGC CAGCAGTAAA 
GCAAAACTTG GTGTACTTAA TAACACAGCT GAGCCTGGAA AATTCCCACT ACTGGGTAAT 
GCTGGATTAA GTAGCCTGAC GGAAGGGGTC TTGGATCTTT TTGCAGTGAA GGCTGTATAC 
ATGGGCCACC CTGGCATTGA TATACACACT GTGTGTGTTC AGAACAAACT GGGTAGCATG 
TTCCTGTCAG AGACTGGTGT GACATTGCTC TATGGGCTTC AGACCACAGA CAACAGATTA 
TTGCACTTCG TGGCACCAAA GCACACAGCT AAAATGCTCT TCAGCGGATT ATTGGAACTC 
ACTAGAGCTG TGAGAAAGAT GAGGAAATTC CCTGACCAAA GACAGCAGTG GCTGCGGAAA 
CAGTACGTCA GCCTTTATCA GGAGGATGGA CGGTATGAAG GCCCAACTTT GGCTCACGCT 
GTGGAGTTGT TTGGTGGCAG ACGGTGGAGT GCTCGAAACC CCAGCCCCGG AACATCAGCA 
AAGAATGCTG AGAAGCCCAA TATGCAGAGA AACAATACCC TGGGCATAAG CACTACCAAG 
AAAAAGAAGA AAATCCTCAT GAGGGGTGAG AGTGGAGAGG TAACTGACGA TGAGATGGCA 
ACCCGAAAGG CCAAGATGCA CAAAGAGTGT CGAAGCCGGA GTGGTTCTGA TCCTCAAGAC 
ATTAATGAAC AAGAAGAATC AGAGGTGAAT GCCATCGCTA ACCCTCCAAA CCCCCTCCCT 
TCCAGAAGAG CCCACTCTTT GACCACAGCT GGGTCCCCCA ACTTGGCTGC CGGGACGTCA 
TCTCCCATCA GGCCAGTGTC CTCCCCTGTG CTGTCTTCTT CAAACAAGAG CCCATCCAGT 
GCTTGGAGCA GTAGTAGCTG GCACGGGCGG ATCAAAGGCG GCATGAAGGG ATTTCAGAGC 
TTCATGGTTT CAGATAGCAA CATGAGTTTT GTTGAATTTG TTGAGCTGTT CAAATCATTC 
AGTGTCAGGA GCCGCAAGGA CCTGAAGGAT CTGTTTGATG TCTATGCAGT GCCCTGCAAC 
CGATCTGGCT CCGAGTCAGC CCCACTCTAC ACCAACCTGA CAATTGATGA AAACACCAGC 
GATCTTCAGC CTGACCTAGA TCTGTTGACC AGAAATGTCT CGGATTTGGG GTTGTTCATT 
AAGAGTAAAC AGCAGCTATC GGACAACCAG AGGCAGATAT CTGATGCCAT TGCTGCTGCA 
AGCATTGTGA CAAATGGCAC TGGGATTGAG AGCACATCTC TGGGCATTTT TGGGGTGGGC 
ATACTTCAGC TCAACGATTT CCTCGTGAAT TGCCAAGGAG AACACTGCAC TTATGATGAA 
ATCCTCAGCA TCATCCAGAA GTTCGAGCCT AGCATCAGTA TGTGTCATCA GGGACTAATG 
TCATTTGAAG GGTTTGCCAG GTTTCTGATG GATAAAGAAA ATTTTGCCTC AAAAAATGAT 
GAGTCACAGG AGAACATTAA AGAACTGCAG CTACCCCTCT CATACTATTA CATCGAATCT 
TCGCACAATA CCTACCTCAC GGGCCATCAG CTCAAAGGAG AATCCTCGGT AGAACTCTAC 
AGCCAGGTCC TTTTGCAAGG CTGTCGAAGT GTAGAATTGG ACTGCTGGGA CGGAGACGAT 
GGGATGCCCA TCATTTATCA TGGACATACG CTGACAACCA AGATCCCCTT CAAGGAAGTG 
GTTGAAGCCA TTGATCGCAG TGCCTTCATC AACTCTGACC TGCCAATCAT CATATCGATT 
GAGAACCACT GTTCATTGCC TCAGCAACGA AAAATGGCAG AAATTTTCAA GACTGTGTTT 
GGAGAAAAGC TGGTGACTAA ATTCTTATTT GAGACTGATT TCTCAGATGA TCCAATGCTT 
CCTTCACCTG ACCAACTCAG AAAGAAAGTT CTTCTTAAAA ACAAGAAGCT AAAAGCCCAT 
CAGACGCCAG TGGATATCTT AAAGCAAAAG GCTCATCAGT TAGCATCTAT GCAAGTGCAG 
GCTTATAATG GTGGGAATGC CAACCCCCGA CCTGCCAATA ATGAGGAAGA GGAAGATGAG 
GAGGACGAAT ATGATTATGA CTATGAATCC CTTTCTGATG CAGATGTCCT TACTGCCTCC 
CCTGCTCCTA ACGGTCAGGA AGACAACATT CTGGAAGACA GACCTGAAAA TAAATCATGT 
AATGACAAGC TTCAGTTTGA ATATAATGAA GAAATCCCAA AGAGGATAAA GAAAGCAGAT 
AACTCTGCTT GCAACAAAGG AAAGGTTTAT GATATGGAAC TGGGAGAAGA ATTTTATCTT 
GATCAGAATA AAAAGGAAAG CAGACAGATT GCACCAGAGC TTTCTGACCT TGTAATCTAT 
TGTCAAGCAG TAAAATTTCC AGGACTGTCA ACTCTAAATG CATCTGGCTC TAGCAGAGGA 
AAAGAAAGGA AAAGCAGGAA GTCCATTTTT GGCAACAATC CGGGCAGAAT GAGCCCAGGG 
GAGACAGCAT CATTTAACAA AACATCTGGA AAAAGTTCCT GTGAAGGCAT TCGACAGACC 
TGGGAGGAAT CTTCTTCCCC TCTCAACCCA ACCACGTCCC TCAGTGCTAT CATTAGAACT 
CCCAAATGTT ATCATATCTC GTCGCTGAAT GAAAATGCCG CCAAACGTCT GTGTCGCAGG 
TATTCTCAGA AACTGACCCA GCACACCGCC TGTCAGCTGC TGAGAACTTA CCCTGCTGCC 
ACCCGCATCG ACTCTTCCAA CCCGAACCCC CTCATGTTCT GGCTCCATGG GATACAGCTT 
GTGGCACTCA ACTACCAGAC TGATGATCTC CCTTTACATT TAAATGCTGC AATGTTTGAG 
GCAAATGGTG GTTGTGGTTA TGTATTGAAA CCTCCAGTTC TGTGGGACAA GAACTGCCCC 
ATGTATCAGA AGTTTTCTCC ACTAGAAAGA GATCTGGACA GCATGGATCC TGCAGTCTAT 
TCTTTAACTA TTGTCTCTGG TCAGAATGTG TGCCCCAGTA ATAGCATGGG AAGCCCGTGC 
ATTGAAGTCG ACGTCCTGGG CATGCCTCTG GACAGCTGCC ATTTCCGCAC AAAGCCCATC 
CATCGAAACA CCCTGAACCC CATGTGGAAC GAGCAGTTTC TGTTCCACGT TCACTTCGAA 
GATCTTGTAT TTCTTCGTTT TGCAGTTGTG GAAAACAATA GTTCAGCGGT AACTGCTCAG 
AGAATCATTC CACTGAAAGC TTTAAAACGA GGATATCGAC ATCTTCAGCT GCGAAACCTT 
CACAATGAAG TCTTGGAGAT TTCTAGTTTA TTCATTAACA GCAGAAGGAT GGAAGAAAAT 
TCCTCTGGCA ATACCATGTC AGCCTCTTCG ATGTTTAATA CAGAAGAAAG AAAATGTTTG 
CAGACTCACA GAGTCACGGT GCATGGGGTC CCAGGGCCAG AGCCCTTTAC CGTTTTCACT 
ATTAATGGAG GCACCAAGGC AAAGCAGCTT CTGCAGCAAA TTCTGACAAA TGAACAAGAC 
ATCAAACCTG TTACCACAGA CTATTTTTTG ATGGAAGAAA AATATTTTAT ATCTAAAGAA 
AAGAATGAAT GTAGGAAACA ACCATTCCAG AGAGCCATTG GTCCAGAAGA GGAGATCATG 
CAAATTTTAA GCAGCTGGTT TCCAGAAGAG GGATACATGG GCAGGATTGT CTTAAAAACC 
CAGCAGGAAA ACCTAGAAGA GAAAAACATT GTTCAAGATG ACAAAGAGGT GATCTTGAGC 
TCAGAGGAGG AGAGTTTCTT TGTCCAAGTG CATGATGTTT CTCCAGAGCA ACCTCGAACA 
GTCATCAAAG CACCCCGCGT CAGCACTGCA CAGGATGTCA TTCAGCAGAC CTTATGCAAA 
GCCAAATATT CCTACAGCAT CCTGAGCAAC CCCAATCCAA GCGACTATGT GCTTTTGGAA 
GAGGTGGTGA AAGACACTAC CAACAAGAAG ACTACCACAC CAAAGTCCTC TCAGCGGGTC 
CTTCTGGATC AGGAGTGTGT GTTTCAAGCC CAAAGCAAGT GGAAAGGTGC AGGAAAATTC 
ATCCTTAAGC TAAAGGAGCA GGTGCAGGCA TCTCGAGAAG ATAAAAAGAA AGGCATTTCT 
TTCGCAAGTG AACTCAAGAA GCTCACCAAG TCAACTAAAC AGCCCCGAGG ACTTACATCA 
CCTTCTCAGC TCTTGACCTC AGAAAGTATC CAAACCAAGG AGGAGAAACC TGTGGGTGGC 
TTGTCCTCCA GTGACACAAT GGATTACCGA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_011539850.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538152.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X9, mRNA.

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CloneID	OHu67755
Clone ID Related Accession (Same CDS sequence)	XM_011539850.3 , XM_011539850.2
Accession Version	XM_011539850.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCAG AAGGAAGTGC AGCAGGAAGA GACTTTGCAG GAATGGAAGA GATCTACAAT 
GCAGTGAGAA GAGAAGAAAC AGAAAATACA GTTGGATCTC TACTCCATTT CCTCACCAAG 
CTCCCAGCCT CCGAGACAGC CCATGGAAGG ATAAGCGTTG GTCCATGCTT AAAGCAATGT 
GTCCGAGACA CTGTATGTGA GTATCGCGCC ACCCTCCAAA GGACTTCAAT ATCGCAGTAC 
ATCACCGGTT CTCTCCTAGA AGCAACCACG TCTTTGGGAG CAAGAAGTGG CCTTCTCAGT 
ACTTTTGGAG GATCCACTGG ACGAATGATG CTGAAAGAAC GCCAGCCAGG CCCCTCTGTG 
GCCAATTCCA ATGCCCTCCC TTCAAGTTCA GCTGGGATCA GCAAGGAGCT GATCGATCTG 
CAGCCTCTCA TCCAGTTCCC AGAGGAAGTC GCCAGCATCC TGATGGAGCA AGAGCAGACT 
ATTTACCGCA GGGTCTTGCC AGTCGACTAC CTTTGCTTCT TAACACGGGA CTTGGGCACT 
CCTGAATGCC AGAGCTCCTT GCCCTGCCTC AAAGCATCCA TCTCAGCGTC GATTCTTACC 
ACTCAGAATG GAGAGCACAA TGCCCTTGAA GATCTGGTGA TGAGGTTTAA TGAGGTGAGC 
TCCTGGGTGA CATGGCTGAT CCTCACGGCA GGCTCCATGG AGGAGAAGCG AGAAGTCTTT 
TCATATTTGG TGCATGTGGC CAAATGCTGC TGGAACATGG GCAACTACAA CGCTGTCATG 
GAGTTCTTGG CTGGCCTCAG GTCAAGAAAA GTTTTAAAAA TGTGGCAGTT CATGGACCAG 
TCTGATATTG AGACCATGAG GAGCCTGAAG GATGCTATGG CCCAGCATGA GTCCTCTTGT 
GAGTACAGAA AGGTGGTGAC ACGTGCCCTG CACATCCCTG GCTGTAAGGT GGTTCCATTC 
TGTGGGGTGT TTCTGAAGGA GCTCTGTGAA GTGCTTGACG GCGCCTCCGG TCTCATGAAG 
CTTTGCCCGC GGTACAATTC CCAAGAAGAA ACTTTAGAGT TTGTAGCAGA TTACAGTGGA 
CAAGATAATT TCTTACAACG AGTGGGACAA AATGGCTTAA AGAATTCGGA GAAGGAGTCC 
ACTGTCAACA GCATCTTTCA GGTCATCCGG AGCTGCAATC GAAGTCTGGA GACAGACGAG 
GAGGACAGCC CCAGTGAAGG AAACAGCTCC AGGAAAAGCT CCTTGAAGGA TAAAAGCCGA 
TGGCAGTTTA TAATTGGAGA TTTGTTGGAT TCAGACAATG ACATCTTTGA GCAATCCAAA 
GAATACGACT CTCATGGTTC AGAGGACTCA CAGAAGGCCT TCGACCATGG GACGGAGCTC 
ATCCCTTGGT ACGTGCTGTC CATCCAAGCC GATGTGCACC AGTTCCTGCT GCAGGGGGCC 
ACGGTCATCC ACTACGACCA GGACACACAC CTCTCTGCCC GCTGCTTCCT CCAGCTTCAG 
CCCGACAATA GCACCTTGAC CTGGGTAAAG CCCACAACTG CCTCCCCAGC CAGCAGTAAA 
GCAAAACTTG GTGTACTTAA TAACACAGCT GAGCCTGGAA AATTCCCACT ACTGGGTAAT 
GCTGGATTAA GTAGCCTGAC GGAAGGGGTC TTGGATCTTT TTGCAGTGAA GGCTGTATAC 
ATGGGCCACC CTGGCATTGA TATACACACT GTGTGTGTTC AGAACAAACT GGGTAGCATG 
TTCCTGTCAG AGACTGGTGT GACATTGCTC TATGGGCTTC AGACCACAGA CAACAGATTA 
TTGCACTTCG TGGCACCAAA GCACACAGCT AAAATGCTCT TCAGCGGATT ATTGGAACTC 
ACTAGAGCTG TGAGAAAGAT GAGGAAATTC CCTGACCAAA GACAGCAGTG GCTGCGGAAA 
CAGTACGTCA GCCTTTATCA GGAGGATGGA CGGTATGAAG GCCCAACTTT GGCTCACGCT 
GTGGAGTTGT TTGGTGGCAG ACGGTGGAGT GCTCGAAACC CCAGCCCCGG AACATCAGCA 
AAGAATGCTG AGAAGCCCAA TATGCAGAGA AACAATACCC TGGGCATAAG CACTACCAAG 
AAAAAGAAGA AAATCCTCAT GAGGGGTGAG AGTGGAGAGG TAACTGACGA TGAGATGGCA 
ACCCGAAAGG CCAAGATGCA CAAAGAGTGT CGAAGCCGGA GTGGTTCTGA TCCTCAAGAC 
ATTAATGAAC AAGAAGAATC AGAGGTGAAT GCCATCGCTA ACCCTCCAAA CCCCCTCCCT 
TCCAGAAGAG CCCACTCTTT GACCACAGCT GGGTCCCCCA ACTTGGCTGC CGGGACGTCA 
TCTCCCATCA GGCCAGTGTC CTCCCCTGTG CTGTCTTCTT CAAACAAGAG CCCATCCAGT 
GCTTGGAGCA GTAGTAGCTG GCACGGGCGG ATCAAAGGCG GCATGAAGGG ATTTCAGAGC 
TTCATGGTTT CAGATAGCAA CATGAGTTTT GTTGAATTTG TTGAGCTGTT CAAATCATTC 
AGTGTCAGGA GCCGCAAGGA CCTGAAGGAT CTGTTTGATG TCTATGCAGT GCCCTGCAAC 
CGATCTGGCT CCGAGTCAGC CCCACTCTAC ACCAACCTGA CAATTGATGA AAACACCAGC 
GATCTTCAGC CTGACCTAGA TCTGTTGACC AGAAATGTCT CGGATTTGGG GTTGTTCATT 
AAGAGTAAAC AGCAGCTATC GGACAACCAG AGGCAGATAT CTGATGCCAT TGCTGCTGCA 
AGCATTGTGA CAAATGGCAC TGGGATTGAG AGCACATCTC TGGGCATTTT TGGGGTGGGC 
ATACTTCAGC TCAACGATTT CCTCGTGAAT TGCCAAGGAG AACACTGCAC TTATGATGAA 
ATCCTCAGCA TCATCCAGAA GTTCGAGCCT AGCATCAGTA TGTGTCATCA GGGACTAATG 
TCATTTGAAG GGTTTGCCAG GTTTCTGATG GATAAAGAAA ATTTTGCCTC AAAAAATGAT 
GAGTCACAGG AGAACATTAA AGAACTGCAG CTACCCCTCT CATACTATTA CATCGAATCT 
TCGCACAATA CCTACCTCAC GGGCCATCAG CTCAAAGGAG AATCCTCGGT AGAACTCTAC 
AGCCAGGTCC TTTTGCAAGG CTGTCGAAGT GTAGAATTGG ACTGCTGGGA CGGAGACGAT 
GGGATGCCCA TCATTTATCA TGGACATACG CTGACAACCA AGATCCCCTT CAAGGAAGTG 
GTTGAAGCCA TTGATCGCAG TGCCTTCATC AACTCTGACC TGCCAATCAT CATATCGATT 
GAGAACCACT GTTCATTGCC TCAGCAACGA AAAATGGCAG AAATTTTCAA GACTGTGTTT 
GGAGAAAAGC TGGTGACTAA ATTCTTATTT GAGACTGATT TCTCAGATGA TCCAATGCTT 
CCTTCACCTG ACCAACTCAG AAAGAAAGTT CTTCTTAAAA ACAAGAAGCT AAAAGCCCAT 
CAGACGCCAG TGGATATCTT AAAGCAAAAG GCTCATCAGT TAGCATCTAT GCAAGTGCAG 
GCTTATAATG GTGGGAATGC CAACCCCCGA CCTGCCAATA ATGAGGAAGA GGAAGATGAG 
GAGGACGAAT ATGATTATGA CTATGAATCC CTTTCTGATG CAGATGTCCT TACTGCCTCC 
CCTGCTCCTA ACGGTCAGGA AGACAACATT CTGGAAGACA GACCTGAAAA TAAATCATGT 
AATGACAAGC TTCAGTTTGA ATATAATGAA GAAATCCCAA AGAGGATAAA GAAAGCAGAT 
AACTCTGCTT GCAACAAAGG AAAGGTTTAT GATATGGAAC TGGGAGAAGA ATTTTATCTT 
GATCAGAATA AAAAGGAAAG CAGACAGATT GCACCAGAGC TTTCTGACCT TGTAATCTAT 
TGTCAAGCAG TAAAATTTCC AGGACTGTCA ACTCTAAATG CATCTGGCTC TAGCAGAGGA 
AAAGAAAGGA AAAGCAGGAA GTCCATTTTT GGCAACAATC CGGGCAGAAT GAGCCCAGGG 
GAGACAGCAT CATTTAACAA AACATCTGGA AAAAGTTCCT GTGAAGGCAT TCGACAGACC 
TGGGAGGAAT CTTCTTCCCC TCTCAACCCA ACCACGTCCC TCAGTGCTAT CATTAGAACT 
CCCAAATGTT ATCATATCTC GTCGCTGAAT GAAAATGCCG CCAAACGTCT GTGTCGCAGG 
TATTCTCAGA AACTGACCCA GCACACCGCC TGTCAGCTGC TGAGAACTTA CCCTGCTGCC 
ACCCGCATCG ACTCTTCCAA CCCGAACCCC CTCATGTTCT GGCTCCATGG GATACAGCTT 
GTGGCACTCA ACTACCAGAC TGATGATCTC CCTTTACATT TAAATGCTGC AATGTTTGAG 
GCAAATGGTG GTTGTGGTTA TGTATTGAAA CCTCCAGTTC TGTGGGACAA GAACTGCCCC 
ATGTATCAGA AGTTTTCTCC ACTAGAAAGA GATCTGGACA GCATGGATCC TGCAGTCTAT 
TCTTTAACTA TTGTCTCTGG TCAGAATGTG TGCCCCAGTA ATAGCATGGG AAGCCCGTGC 
ATTGAAGTCG ACGTCCTGGG CATGCCTCTG GACAGCTGCC ATTTCCGCAC AAAGCCCATC 
CATCGAAACA CCCTGAACCC CATGTGGAAC GAGCAGTTTC TGTTCCACGT TCACTTCGAA 
GATCTTGTAT TTCTTCGTTT TGCAGTTGTG GAAAACAATA GTTCAGCGGT AACTGCTCAG 
AGAATCATTC CACTGAAAGC TTTAAAACGA GGATATCGAC ATCTTCAGCT GCGAAACCTT 
CACAATGAAG TCTTGGAGAT TTCTAGTTTA TTCATTAACA GCAGAAGGAT GGAAGAAAAT 
TCCTCTGGCA ATACCATGTC AGCCTCTTCG ATGTTTAATA CAGAAGAAAG AAAATGTTTG 
CAGACTCACA GAGTCACGGT GCATGGGGTC CCAGGGCCAG AGCCCTTTAC CGTTTTCACT 
ATTAATGGAG GCACCAAGGC AAAGCAGCTT CTGCAGCAAA TTCTGACAAA TGAACAAGAC 
ATCAAACCTG TTACCACAGA CTATTTTTTG ATGGAAGAAA AATATTTTAT ATCTAAAGAA 
AAGAATGAAT GTAGGAAACA ACCATTCCAG AGAGCCATTG GTCCAGAAGA GGAGATCATG 
CAAATTTTAA GCAGCTGGTT TCCAGAAGAG GGATACATGG GCAGGATTGT CTTAAAAACC 
CAGCAGGAAA ACCTAGAAGA GAAAAACATT GTTCAAGATG ACAAAGAGGT GATCTTGAGC 
TCAGAGGAGG AGAGTTTCTT TGTCCAAGTG CATGATGTTT CTCCAGAGCA ACCTCGAACA 
GTCATCAAAG CACCCCGCGT CAGCACTGCA CAGGATGTCA TTCAGCAGAC CTTATGCAAA 
GCCAAATATT CCTACAGCAT CCTGAGCAAC CCCAATCCAA GCGACTATGT GCTTTTGGAA 
GAGGTGGTGA AAGACACTAC CAACAAGAAG ACTACCACAC CAAAGTCCTC TCAGCGGGTC 
CTTCTGGATC AGGAGTGTGT GTTTCAAGCC CAAAGCAAGT GGAAAGGTGC AGGAAAATTC 
ATCCTTAAGC TAAAGGAGCA GGTGCAGGCA TCTCGAGAAG ATAAAAAGAA AGGCATTTCT 
TTCGCAAGTG AACTCAAGAA GCTCACCAAG TCAACTAAAC AGCCCCGAGG ACTTACATCA 
CCTTCTCAGC TCTTGACCTC AGAAAGTATC CAAACCAAGG AGGAGAAACC TGTGGGTGGC 
TTGTCCTCCA GTGACACAAT GGATTACCGA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538152.1
CDS	1383..7178
Translation	MVSEGSAAGRDFAGMEEIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDADVLTASPAPNGQEDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_011539850.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539850.2

ORF Insert Sequence:

ATGGTTTCAG AAGGAAGTGC AGCAGGAAGA GACTTTGCAG GAATGGAAGA GATCTACAAT 
GCAGTGAGAA GAGAAGAAAC AGAAAATACA GTTGGATCTC TACTCCATTT CCTCACCAAG 
CTCCCAGCCT CCGAGACAGC CCATGGAAGG ATAAGCGTTG GTCCATGCTT AAAGCAATGT 
GTCCGAGACA CTGTATGTGA GTATCGCGCC ACCCTCCAAA GGACTTCAAT ATCGCAGTAC 
ATCACCGGTT CTCTCCTAGA AGCAACCACG TCTTTGGGAG CAAGAAGTGG CCTTCTCAGT 
ACTTTTGGAG GATCCACTGG ACGAATGATG CTGAAAGAAC GCCAGCCAGG CCCCTCTGTG 
GCCAATTCCA ATGCCCTCCC TTCAAGTTCA GCTGGGATCA GCAAGGAGCT GATCGATCTG 
CAGCCTCTCA TCCAGTTCCC AGAGGAAGTC GCCAGCATCC TGATGGAGCA AGAGCAGACT 
ATTTACCGCA GGGTCTTGCC AGTCGACTAC CTTTGCTTCT TAACACGGGA CTTGGGCACT 
CCTGAATGCC AGAGCTCCTT GCCCTGCCTC AAAGCATCCA TCTCAGCGTC GATTCTTACC 
ACTCAGAATG GAGAGCACAA TGCCCTTGAA GATCTGGTGA TGAGGTTTAA TGAGGTGAGC 
TCCTGGGTGA CATGGCTGAT CCTCACGGCA GGCTCCATGG AGGAGAAGCG AGAAGTCTTT 
TCATATTTGG TGCATGTGGC CAAATGCTGC TGGAACATGG GCAACTACAA CGCTGTCATG 
GAGTTCTTGG CTGGCCTCAG GTCAAGAAAA GTTTTAAAAA TGTGGCAGTT CATGGACCAG 
TCTGATATTG AGACCATGAG GAGCCTGAAG GATGCTATGG CCCAGCATGA GTCCTCTTGT 
GAGTACAGAA AGGTGGTGAC ACGTGCCCTG CACATCCCTG GCTGTAAGGT GGTTCCATTC 
TGTGGGGTGT TTCTGAAGGA GCTCTGTGAA GTGCTTGACG GCGCCTCCGG TCTCATGAAG 
CTTTGCCCGC GGTACAATTC CCAAGAAGAA ACTTTAGAGT TTGTAGCAGA TTACAGTGGA 
CAAGATAATT TCTTACAACG AGTGGGACAA AATGGCTTAA AGAATTCGGA GAAGGAGTCC 
ACTGTCAACA GCATCTTTCA GGTCATCCGG AGCTGCAATC GAAGTCTGGA GACAGACGAG 
GAGGACAGCC CCAGTGAAGG AAACAGCTCC AGGAAAAGCT CCTTGAAGGA TAAAAGCCGA 
TGGCAGTTTA TAATTGGAGA TTTGTTGGAT TCAGACAATG ACATCTTTGA GCAATCCAAA 
GAATACGACT CTCATGGTTC AGAGGACTCA CAGAAGGCCT TCGACCATGG GACGGAGCTC 
ATCCCTTGGT ACGTGCTGTC CATCCAAGCC GATGTGCACC AGTTCCTGCT GCAGGGGGCC 
ACGGTCATCC ACTACGACCA GGACACACAC CTCTCTGCCC GCTGCTTCCT CCAGCTTCAG 
CCCGACAATA GCACCTTGAC CTGGGTAAAG CCCACAACTG CCTCCCCAGC CAGCAGTAAA 
GCAAAACTTG GTGTACTTAA TAACACAGCT GAGCCTGGAA AATTCCCACT ACTGGGTAAT 
GCTGGATTAA GTAGCCTGAC GGAAGGGGTC TTGGATCTTT TTGCAGTGAA GGCTGTATAC 
ATGGGCCACC CTGGCATTGA TATACACACT GTGTGTGTTC AGAACAAACT GGGTAGCATG 
TTCCTGTCAG AGACTGGTGT GACATTGCTC TATGGGCTTC AGACCACAGA CAACAGATTA 
TTGCACTTCG TGGCACCAAA GCACACAGCT AAAATGCTCT TCAGCGGATT ATTGGAACTC 
ACTAGAGCTG TGAGAAAGAT GAGGAAATTC CCTGACCAAA GACAGCAGTG GCTGCGGAAA 
CAGTACGTCA GCCTTTATCA GGAGGATGGA CGGTATGAAG GCCCAACTTT GGCTCACGCT 
GTGGAGTTGT TTGGTGGCAG ACGGTGGAGT GCTCGAAACC CCAGCCCCGG AACATCAGCA 
AAGAATGCTG AGAAGCCCAA TATGCAGAGA AACAATACCC TGGGCATAAG CACTACCAAG 
AAAAAGAAGA AAATCCTCAT GAGGGGTGAG AGTGGAGAGG TAACTGACGA TGAGATGGCA 
ACCCGAAAGG CCAAGATGCA CAAAGAGTGT CGAAGCCGGA GTGGTTCTGA TCCTCAAGAC 
ATTAATGAAC AAGAAGAATC AGAGGTGAAT GCCATCGCTA ACCCTCCAAA CCCCCTCCCT 
TCCAGAAGAG CCCACTCTTT GACCACAGCT GGGTCCCCCA ACTTGGCTGC CGGGACGTCA 
TCTCCCATCA GGCCAGTGTC CTCCCCTGTG CTGTCTTCTT CAAACAAGAG CCCATCCAGT 
GCTTGGAGCA GTAGTAGCTG GCACGGGCGG ATCAAAGGCG GCATGAAGGG ATTTCAGAGC 
TTCATGGTTT CAGATAGCAA CATGAGTTTT GTTGAATTTG TTGAGCTGTT CAAATCATTC 
AGTGTCAGGA GCCGCAAGGA CCTGAAGGAT CTGTTTGATG TCTATGCAGT GCCCTGCAAC 
CGATCTGGCT CCGAGTCAGC CCCACTCTAC ACCAACCTGA CAATTGATGA AAACACCAGC 
GATCTTCAGC CTGACCTAGA TCTGTTGACC AGAAATGTCT CGGATTTGGG GTTGTTCATT 
AAGAGTAAAC AGCAGCTATC GGACAACCAG AGGCAGATAT CTGATGCCAT TGCTGCTGCA 
AGCATTGTGA CAAATGGCAC TGGGATTGAG AGCACATCTC TGGGCATTTT TGGGGTGGGC 
ATACTTCAGC TCAACGATTT CCTCGTGAAT TGCCAAGGAG AACACTGCAC TTATGATGAA 
ATCCTCAGCA TCATCCAGAA GTTCGAGCCT AGCATCAGTA TGTGTCATCA GGGACTAATG 
TCATTTGAAG GGTTTGCCAG GTTTCTGATG GATAAAGAAA ATTTTGCCTC AAAAAATGAT 
GAGTCACAGG AGAACATTAA AGAACTGCAG CTACCCCTCT CATACTATTA CATCGAATCT 
TCGCACAATA CCTACCTCAC GGGCCATCAG CTCAAAGGAG AATCCTCGGT AGAACTCTAC 
AGCCAGGTCC TTTTGCAAGG CTGTCGAAGT GTAGAATTGG ACTGCTGGGA CGGAGACGAT 
GGGATGCCCA TCATTTATCA TGGACATACG CTGACAACCA AGATCCCCTT CAAGGAAGTG 
GTTGAAGCCA TTGATCGCAG TGCCTTCATC AACTCTGACC TGCCAATCAT CATATCGATT 
GAGAACCACT GTTCATTGCC TCAGCAACGA AAAATGGCAG AAATTTTCAA GACTGTGTTT 
GGAGAAAAGC TGGTGACTAA ATTCTTATTT GAGACTGATT TCTCAGATGA TCCAATGCTT 
CCTTCACCTG ACCAACTCAG AAAGAAAGTT CTTCTTAAAA ACAAGAAGCT AAAAGCCCAT 
CAGACGCCAG TGGATATCTT AAAGCAAAAG GCTCATCAGT TAGCATCTAT GCAAGTGCAG 
GCTTATAATG GTGGGAATGC CAACCCCCGA CCTGCCAATA ATGAGGAAGA GGAAGATGAG 
GAGGACGAAT ATGATTATGA CTATGAATCC CTTTCTGATG CAGATGTCCT TACTGCCTCC 
CCTGCTCCTA ACGGTCAGGA AGACAACATT CTGGAAGACA GACCTGAAAA TAAATCATGT 
AATGACAAGC TTCAGTTTGA ATATAATGAA GAAATCCCAA AGAGGATAAA GAAAGCAGAT 
AACTCTGCTT GCAACAAAGG AAAGGTTTAT GATATGGAAC TGGGAGAAGA ATTTTATCTT 
GATCAGAATA AAAAGGAAAG CAGACAGATT GCACCAGAGC TTTCTGACCT TGTAATCTAT 
TGTCAAGCAG TAAAATTTCC AGGACTGTCA ACTCTAAATG CATCTGGCTC TAGCAGAGGA 
AAAGAAAGGA AAAGCAGGAA GTCCATTTTT GGCAACAATC CGGGCAGAAT GAGCCCAGGG 
GAGACAGCAT CATTTAACAA AACATCTGGA AAAAGTTCCT GTGAAGGCAT TCGACAGACC 
TGGGAGGAAT CTTCTTCCCC TCTCAACCCA ACCACGTCCC TCAGTGCTAT CATTAGAACT 
CCCAAATGTT ATCATATCTC GTCGCTGAAT GAAAATGCCG CCAAACGTCT GTGTCGCAGG 
TATTCTCAGA AACTGACCCA GCACACCGCC TGTCAGCTGC TGAGAACTTA CCCTGCTGCC 
ACCCGCATCG ACTCTTCCAA CCCGAACCCC CTCATGTTCT GGCTCCATGG GATACAGCTT 
GTGGCACTCA ACTACCAGAC TGATGATCTC CCTTTACATT TAAATGCTGC AATGTTTGAG 
GCAAATGGTG GTTGTGGTTA TGTATTGAAA CCTCCAGTTC TGTGGGACAA GAACTGCCCC 
ATGTATCAGA AGTTTTCTCC ACTAGAAAGA GATCTGGACA GCATGGATCC TGCAGTCTAT 
TCTTTAACTA TTGTCTCTGG TCAGAATGTG TGCCCCAGTA ATAGCATGGG AAGCCCGTGC 
ATTGAAGTCG ACGTCCTGGG CATGCCTCTG GACAGCTGCC ATTTCCGCAC AAAGCCCATC 
CATCGAAACA CCCTGAACCC CATGTGGAAC GAGCAGTTTC TGTTCCACGT TCACTTCGAA 
GATCTTGTAT TTCTTCGTTT TGCAGTTGTG GAAAACAATA GTTCAGCGGT AACTGCTCAG 
AGAATCATTC CACTGAAAGC TTTAAAACGA GGATATCGAC ATCTTCAGCT GCGAAACCTT 
CACAATGAAG TCTTGGAGAT TTCTAGTTTA TTCATTAACA GCAGAAGGAT GGAAGAAAAT 
TCCTCTGGCA ATACCATGTC AGCCTCTTCG ATGTTTAATA CAGAAGAAAG AAAATGTTTG 
CAGACTCACA GAGTCACGGT GCATGGGGTC CCAGGGCCAG AGCCCTTTAC CGTTTTCACT 
ATTAATGGAG GCACCAAGGC AAAGCAGCTT CTGCAGCAAA TTCTGACAAA TGAACAAGAC 
ATCAAACCTG TTACCACAGA CTATTTTTTG ATGGAAGAAA AATATTTTAT ATCTAAAGAA 
AAGAATGAAT GTAGGAAACA ACCATTCCAG AGAGCCATTG GTCCAGAAGA GGAGATCATG 
CAAATTTTAA GCAGCTGGTT TCCAGAAGAG GGATACATGG GCAGGATTGT CTTAAAAACC 
CAGCAGGAAA ACCTAGAAGA GAAAAACATT GTTCAAGATG ACAAAGAGGT GATCTTGAGC 
TCAGAGGAGG AGAGTTTCTT TGTCCAAGTG CATGATGTTT CTCCAGAGCA ACCTCGAACA 
GTCATCAAAG CACCCCGCGT CAGCACTGCA CAGGATGTCA TTCAGCAGAC CTTATGCAAA 
GCCAAATATT CCTACAGCAT CCTGAGCAAC CCCAATCCAA GCGACTATGT GCTTTTGGAA 
GAGGTGGTGA AAGACACTAC CAACAAGAAG ACTACCACAC CAAAGTCCTC TCAGCGGGTC 
CTTCTGGATC AGGAGTGTGT GTTTCAAGCC CAAAGCAAGT GGAAAGGTGC AGGAAAATTC 
ATCCTTAAGC TAAAGGAGCA GGTGCAGGCA TCTCGAGAAG ATAAAAAGAA AGGCATTTCT 
TTCGCAAGTG AACTCAAGAA GCTCACCAAG TCAACTAAAC AGCCCCGAGG ACTTACATCA 
CCTTCTCAGC TCTTGACCTC AGAAAGTATC CAAACCAAGG AGGAGAAACC TGTGGGTGGC 
TTGTCCTCCA GTGACACAAT GGATTACCGA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_011539851.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538153.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X10, mRNA.

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CloneID	OHu67756
Clone ID Related Accession (Same CDS sequence)	XM_011539851.2 , XM_011539851.3
Accession Version	XM_011539851.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539851.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGGTT TTGGCATTTC TCAAGGTAAA 
AGAAGTAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538153.1
CDS	585..5456
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDADVLTASPAPNGQEGFGISQGKRSK

Target ORF information:

RefSeq Version	XM_011539851.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539851.2

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGGTT TTGGCATTTC TCAAGGTAAA 
AGAAGTAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_011539851.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538153.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X10, mRNA.

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CloneID	OHu67756
Clone ID Related Accession (Same CDS sequence)	XM_011539851.2 , XM_011539851.3
Accession Version	XM_011539851.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011539851.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGGTT TTGGCATTTC TCAAGGTAAA 
AGAAGTAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538153.1
CDS	585..5456
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDADVLTASPAPNGQEGFGISQGKRSK

Target ORF information:

RefSeq Version	XM_011539851.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539851.3

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGGTT TTGGCATTTC TCAAGGTAAA 
AGAAGTAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_011539852.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538154.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu67757
Clone ID Related Accession (Same CDS sequence)	XM_011539852.2 , XM_011539852.3
Accession Version	XM_011539852.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGATGTC 
CTTACTGCCT CCCCTGCTCC TAACGGTCAG GAAGGTTTTG GCATTTCTCA AGGTAAAAGA 
AGTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538154.1
CDS	585..5453
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDDVLTASPAPNGQEGFGISQGKRSK

Target ORF information:

RefSeq Version	XM_011539852.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539852.2

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGATGTC 
CTTACTGCCT CCCCTGCTCC TAACGGTCAG GAAGGTTTTG GCATTTCTCA AGGTAAAAGA 
AGTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_011539852.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538154.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X11, mRNA.

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CloneID	OHu67757
Clone ID Related Accession (Same CDS sequence)	XM_011539852.2 , XM_011539852.3
Accession Version	XM_011539852.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011539852.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGATGTC 
CTTACTGCCT CCCCTGCTCC TAACGGTCAG GAAGGTTTTG GCATTTCTCA AGGTAAAAGA 
AGTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538154.1
CDS	585..5453
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDDVLTASPAPNGQEGFGISQGKRSK

Target ORF information:

RefSeq Version	XM_011539852.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539852.3

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGATGTC 
CTTACTGCCT CCCCTGCTCC TAACGGTCAG GAAGGTTTTG GCATTTCTCA AGGTAAAAGA 
AGTAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( NM_016341.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057425.3 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant 1, mRNA.

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CloneID	OHu25512
Clone ID Related Accession (Same CDS sequence)	NM_016341.4 , NM_016341.3
Accession Version	NM_016341.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL365510.32, AB040949.2, AK074239.1, AF117948.1, AL139124.12 and AL355341.19. On Jun 2, 2019 this sequence version replaced NM_016341.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1; also known as PLCepsilon1a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC151854.1, AB040949.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGACAAC 
ATTCTGGAAG ACAGACCTGA AAATAAATCA TGTAATGACA AGCTTCAGTT TGAATATAAT 
GAAGAAATCC CAAAGAGGAT AAAGAAAGCA GATAACTCTG CTTGCAACAA AGGAAAGGTT 
TATGATATGG AACTGGGAGA AGAATTTTAT CTTGATCAGA ATAAAAAGGA AAGCAGACAG 
ATTGCACCAG AGCTTTCTGA CCTTGTAATC TATTGTCAAG CAGTAAAATT TCCAGGACTG 
TCAACTCTAA ATGCATCTGG CTCTAGCAGA GGAAAAGAAA GGAAAAGCAG GAAGTCCATT 
TTTGGCAACA ATCCGGGCAG AATGAGCCCA GGGGAGACAG CATCATTTAA CAAAACATCT 
GGAAAAAGTT CCTGTGAAGG CATTCGACAG ACCTGGGAGG AATCTTCTTC CCCTCTCAAC 
CCAACCACGT CCCTCAGTGC TATCATTAGA ACTCCCAAAT GTTATCATAT CTCGTCGCTG 
AATGAAAATG CCGCCAAACG TCTGTGTCGC AGGTATTCTC AGAAACTGAC CCAGCACACC 
GCCTGTCAGC TGCTGAGAAC TTACCCTGCT GCCACCCGCA TCGACTCTTC CAACCCGAAC 
CCCCTCATGT TCTGGCTCCA TGGGATACAG CTTGTGGCAC TCAACTACCA GACTGATGAT 
CTCCCTTTAC ATTTAAATGC TGCAATGTTT GAGGCAAATG GTGGTTGTGG TTATGTATTG 
AAACCTCCAG TTCTGTGGGA CAAGAACTGC CCCATGTATC AGAAGTTTTC TCCACTAGAA 
AGAGATCTGG ACAGCATGGA TCCTGCAGTC TATTCTTTAA CTATTGTCTC TGGTCAGAAT 
GTGTGCCCCA GTAATAGCAT GGGAAGCCCG TGCATTGAAG TCGACGTCCT GGGCATGCCT 
CTGGACAGCT GCCATTTCCG CACAAAGCCC ATCCATCGAA ACACCCTGAA CCCCATGTGG 
AACGAGCAGT TTCTGTTCCA CGTTCACTTC GAAGATCTTG TATTTCTTCG TTTTGCAGTT 
GTGGAAAACA ATAGTTCAGC GGTAACTGCT CAGAGAATCA TTCCACTGAA AGCTTTAAAA 
CGAGGATATC GACATCTTCA GCTGCGAAAC CTTCACAATG AAGTCTTGGA GATTTCTAGT 
TTATTCATTA ACAGCAGAAG GATGGAAGAA AATTCCTCTG GCAATACCAT GTCAGCCTCT 
TCGATGTTTA ATACAGAAGA AAGAAAATGT TTGCAGACTC ACAGAGTCAC GGTGCATGGG 
GTCCCAGGGC CAGAGCCCTT TACCGTTTTC ACTATTAATG GAGGCACCAA GGCAAAGCAG 
CTTCTGCAGC AAATTCTGAC AAATGAACAA GACATCAAAC CTGTTACCAC AGACTATTTT 
TTGATGGAAG AAAAATATTT TATATCTAAA GAAAAGAATG AATGTAGGAA ACAACCATTC 
CAGAGAGCCA TTGGTCCAGA AGAGGAGATC ATGCAAATTT TAAGCAGCTG GTTTCCAGAA 
GAGGGATACA TGGGCAGGAT TGTCTTAAAA ACCCAGCAGG AAAACCTAGA AGAGAAAAAC 
ATTGTTCAAG ATGACAAAGA GGTGATCTTG AGCTCAGAGG AGGAGAGTTT CTTTGTCCAA 
GTGCATGATG TTTCTCCAGA GCAACCTCGA ACAGTCATCA AAGCACCCCG CGTCAGCACT 
GCACAGGATG TCATTCAGCA GACCTTATGC AAAGCCAAAT ATTCCTACAG CATCCTGAGC 
AACCCCAATC CAAGCGACTA TGTGCTTTTG GAAGAGGTGG TGAAAGACAC TACCAACAAG 
AAGACTACCA CACCAAAGTC CTCTCAGCGG GTCCTTCTGG ATCAGGAGTG TGTGTTTCAA 
GCCCAAAGCA AGTGGAAAGG TGCAGGAAAA TTCATCCTTA AGCTAAAGGA GCAGGTGCAG 
GCATCTCGAG AAGATAAAAA GAAAGGCATT TCTTTCGCAA GTGAACTCAA GAAGCTCACC 
AAGTCAACTA AACAGCCCCG AGGACTTACA TCACCTTCTC AGCTCTTGAC CTCAGAAAGT 
ATCCAAACCA AGGAGGAGAA ACCTGTGGGT GGCTTGTCCT CCAGTGACAC AATGGATTAC 
CGACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057425.3
CDS	693..7601
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	NM_016341.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016341.4

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGACAAC 
ATTCTGGAAG ACAGACCTGA AAATAAATCA TGTAATGACA AGCTTCAGTT TGAATATAAT 
GAAGAAATCC CAAAGAGGAT AAAGAAAGCA GATAACTCTG CTTGCAACAA AGGAAAGGTT 
TATGATATGG AACTGGGAGA AGAATTTTAT CTTGATCAGA ATAAAAAGGA AAGCAGACAG 
ATTGCACCAG AGCTTTCTGA CCTTGTAATC TATTGTCAAG CAGTAAAATT TCCAGGACTG 
TCAACTCTAA ATGCATCTGG CTCTAGCAGA GGAAAAGAAA GGAAAAGCAG GAAGTCCATT 
TTTGGCAACA ATCCGGGCAG AATGAGCCCA GGGGAGACAG CATCATTTAA CAAAACATCT 
GGAAAAAGTT CCTGTGAAGG CATTCGACAG ACCTGGGAGG AATCTTCTTC CCCTCTCAAC 
CCAACCACGT CCCTCAGTGC TATCATTAGA ACTCCCAAAT GTTATCATAT CTCGTCGCTG 
AATGAAAATG CCGCCAAACG TCTGTGTCGC AGGTATTCTC AGAAACTGAC CCAGCACACC 
GCCTGTCAGC TGCTGAGAAC TTACCCTGCT GCCACCCGCA TCGACTCTTC CAACCCGAAC 
CCCCTCATGT TCTGGCTCCA TGGGATACAG CTTGTGGCAC TCAACTACCA GACTGATGAT 
CTCCCTTTAC ATTTAAATGC TGCAATGTTT GAGGCAAATG GTGGTTGTGG TTATGTATTG 
AAACCTCCAG TTCTGTGGGA CAAGAACTGC CCCATGTATC AGAAGTTTTC TCCACTAGAA 
AGAGATCTGG ACAGCATGGA TCCTGCAGTC TATTCTTTAA CTATTGTCTC TGGTCAGAAT 
GTGTGCCCCA GTAATAGCAT GGGAAGCCCG TGCATTGAAG TCGACGTCCT GGGCATGCCT 
CTGGACAGCT GCCATTTCCG CACAAAGCCC ATCCATCGAA ACACCCTGAA CCCCATGTGG 
AACGAGCAGT TTCTGTTCCA CGTTCACTTC GAAGATCTTG TATTTCTTCG TTTTGCAGTT 
GTGGAAAACA ATAGTTCAGC GGTAACTGCT CAGAGAATCA TTCCACTGAA AGCTTTAAAA 
CGAGGATATC GACATCTTCA GCTGCGAAAC CTTCACAATG AAGTCTTGGA GATTTCTAGT 
TTATTCATTA ACAGCAGAAG GATGGAAGAA AATTCCTCTG GCAATACCAT GTCAGCCTCT 
TCGATGTTTA ATACAGAAGA AAGAAAATGT TTGCAGACTC ACAGAGTCAC GGTGCATGGG 
GTCCCAGGGC CAGAGCCCTT TACCGTTTTC ACTATTAATG GAGGCACCAA GGCAAAGCAG 
CTTCTGCAGC AAATTCTGAC AAATGAACAA GACATCAAAC CTGTTACCAC AGACTATTTT 
TTGATGGAAG AAAAATATTT TATATCTAAA GAAAAGAATG AATGTAGGAA ACAACCATTC 
CAGAGAGCCA TTGGTCCAGA AGAGGAGATC ATGCAAATTT TAAGCAGCTG GTTTCCAGAA 
GAGGGATACA TGGGCAGGAT TGTCTTAAAA ACCCAGCAGG AAAACCTAGA AGAGAAAAAC 
ATTGTTCAAG ATGACAAAGA GGTGATCTTG AGCTCAGAGG AGGAGAGTTT CTTTGTCCAA 
GTGCATGATG TTTCTCCAGA GCAACCTCGA ACAGTCATCA AAGCACCCCG CGTCAGCACT 
GCACAGGATG TCATTCAGCA GACCTTATGC AAAGCCAAAT ATTCCTACAG CATCCTGAGC 
AACCCCAATC CAAGCGACTA TGTGCTTTTG GAAGAGGTGG TGAAAGACAC TACCAACAAG 
AAGACTACCA CACCAAAGTC CTCTCAGCGG GTCCTTCTGG ATCAGGAGTG TGTGTTTCAA 
GCCCAAAGCA AGTGGAAAGG TGCAGGAAAA TTCATCCTTA AGCTAAAGGA GCAGGTGCAG 
GCATCTCGAG AAGATAAAAA GAAAGGCATT TCTTTCGCAA GTGAACTCAA GAAGCTCACC 
AAGTCAACTA AACAGCCCCG AGGACTTACA TCACCTTCTC AGCTCTTGAC CTCAGAAAGT 
ATCCAAACCA AGGAGGAGAA ACCTGTGGGT GGCTTGTCCT CCAGTGACAC AATGGATTAC 
CGACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( NM_016341.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057425.3 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant 1, mRNA.

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CloneID	OHu25512
Clone ID Related Accession (Same CDS sequence)	NM_016341.4 , NM_016341.3
Accession Version	NM_016341.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB040949.2, AK074239.1 and AF117948.1. This sequence is a reference standard in the RefSeqGene project. On or before Mar 29, 2008 this sequence version replaced XM_001124973.2, NM_016341.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1; also known as PLCepsilon1a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC151854.1, AB040949.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGACAAC 
ATTCTGGAAG ACAGACCTGA AAATAAATCA TGTAATGACA AGCTTCAGTT TGAATATAAT 
GAAGAAATCC CAAAGAGGAT AAAGAAAGCA GATAACTCTG CTTGCAACAA AGGAAAGGTT 
TATGATATGG AACTGGGAGA AGAATTTTAT CTTGATCAGA ATAAAAAGGA AAGCAGACAG 
ATTGCACCAG AGCTTTCTGA CCTTGTAATC TATTGTCAAG CAGTAAAATT TCCAGGACTG 
TCAACTCTAA ATGCATCTGG CTCTAGCAGA GGAAAAGAAA GGAAAAGCAG GAAGTCCATT 
TTTGGCAACA ATCCGGGCAG AATGAGCCCA GGGGAGACAG CATCATTTAA CAAAACATCT 
GGAAAAAGTT CCTGTGAAGG CATTCGACAG ACCTGGGAGG AATCTTCTTC CCCTCTCAAC 
CCAACCACGT CCCTCAGTGC TATCATTAGA ACTCCCAAAT GTTATCATAT CTCGTCGCTG 
AATGAAAATG CCGCCAAACG TCTGTGTCGC AGGTATTCTC AGAAACTGAC CCAGCACACC 
GCCTGTCAGC TGCTGAGAAC TTACCCTGCT GCCACCCGCA TCGACTCTTC CAACCCGAAC 
CCCCTCATGT TCTGGCTCCA TGGGATACAG CTTGTGGCAC TCAACTACCA GACTGATGAT 
CTCCCTTTAC ATTTAAATGC TGCAATGTTT GAGGCAAATG GTGGTTGTGG TTATGTATTG 
AAACCTCCAG TTCTGTGGGA CAAGAACTGC CCCATGTATC AGAAGTTTTC TCCACTAGAA 
AGAGATCTGG ACAGCATGGA TCCTGCAGTC TATTCTTTAA CTATTGTCTC TGGTCAGAAT 
GTGTGCCCCA GTAATAGCAT GGGAAGCCCG TGCATTGAAG TCGACGTCCT GGGCATGCCT 
CTGGACAGCT GCCATTTCCG CACAAAGCCC ATCCATCGAA ACACCCTGAA CCCCATGTGG 
AACGAGCAGT TTCTGTTCCA CGTTCACTTC GAAGATCTTG TATTTCTTCG TTTTGCAGTT 
GTGGAAAACA ATAGTTCAGC GGTAACTGCT CAGAGAATCA TTCCACTGAA AGCTTTAAAA 
CGAGGATATC GACATCTTCA GCTGCGAAAC CTTCACAATG AAGTCTTGGA GATTTCTAGT 
TTATTCATTA ACAGCAGAAG GATGGAAGAA AATTCCTCTG GCAATACCAT GTCAGCCTCT 
TCGATGTTTA ATACAGAAGA AAGAAAATGT TTGCAGACTC ACAGAGTCAC GGTGCATGGG 
GTCCCAGGGC CAGAGCCCTT TACCGTTTTC ACTATTAATG GAGGCACCAA GGCAAAGCAG 
CTTCTGCAGC AAATTCTGAC AAATGAACAA GACATCAAAC CTGTTACCAC AGACTATTTT 
TTGATGGAAG AAAAATATTT TATATCTAAA GAAAAGAATG AATGTAGGAA ACAACCATTC 
CAGAGAGCCA TTGGTCCAGA AGAGGAGATC ATGCAAATTT TAAGCAGCTG GTTTCCAGAA 
GAGGGATACA TGGGCAGGAT TGTCTTAAAA ACCCAGCAGG AAAACCTAGA AGAGAAAAAC 
ATTGTTCAAG ATGACAAAGA GGTGATCTTG AGCTCAGAGG AGGAGAGTTT CTTTGTCCAA 
GTGCATGATG TTTCTCCAGA GCAACCTCGA ACAGTCATCA AAGCACCCCG CGTCAGCACT 
GCACAGGATG TCATTCAGCA GACCTTATGC AAAGCCAAAT ATTCCTACAG CATCCTGAGC 
AACCCCAATC CAAGCGACTA TGTGCTTTTG GAAGAGGTGG TGAAAGACAC TACCAACAAG 
AAGACTACCA CACCAAAGTC CTCTCAGCGG GTCCTTCTGG ATCAGGAGTG TGTGTTTCAA 
GCCCAAAGCA AGTGGAAAGG TGCAGGAAAA TTCATCCTTA AGCTAAAGGA GCAGGTGCAG 
GCATCTCGAG AAGATAAAAA GAAAGGCATT TCTTTCGCAA GTGAACTCAA GAAGCTCACC 
AAGTCAACTA AACAGCCCCG AGGACTTACA TCACCTTCTC AGCTCTTGAC CTCAGAAAGT 
ATCCAAACCA AGGAGGAGAA ACCTGTGGGT GGCTTGTCCT CCAGTGACAC AATGGATTAC 
CGACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057425.3
CDS	635..7543
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	NM_016341.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016341.3

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGACAAC 
ATTCTGGAAG ACAGACCTGA AAATAAATCA TGTAATGACA AGCTTCAGTT TGAATATAAT 
GAAGAAATCC CAAAGAGGAT AAAGAAAGCA GATAACTCTG CTTGCAACAA AGGAAAGGTT 
TATGATATGG AACTGGGAGA AGAATTTTAT CTTGATCAGA ATAAAAAGGA AAGCAGACAG 
ATTGCACCAG AGCTTTCTGA CCTTGTAATC TATTGTCAAG CAGTAAAATT TCCAGGACTG 
TCAACTCTAA ATGCATCTGG CTCTAGCAGA GGAAAAGAAA GGAAAAGCAG GAAGTCCATT 
TTTGGCAACA ATCCGGGCAG AATGAGCCCA GGGGAGACAG CATCATTTAA CAAAACATCT 
GGAAAAAGTT CCTGTGAAGG CATTCGACAG ACCTGGGAGG AATCTTCTTC CCCTCTCAAC 
CCAACCACGT CCCTCAGTGC TATCATTAGA ACTCCCAAAT GTTATCATAT CTCGTCGCTG 
AATGAAAATG CCGCCAAACG TCTGTGTCGC AGGTATTCTC AGAAACTGAC CCAGCACACC 
GCCTGTCAGC TGCTGAGAAC TTACCCTGCT GCCACCCGCA TCGACTCTTC CAACCCGAAC 
CCCCTCATGT TCTGGCTCCA TGGGATACAG CTTGTGGCAC TCAACTACCA GACTGATGAT 
CTCCCTTTAC ATTTAAATGC TGCAATGTTT GAGGCAAATG GTGGTTGTGG TTATGTATTG 
AAACCTCCAG TTCTGTGGGA CAAGAACTGC CCCATGTATC AGAAGTTTTC TCCACTAGAA 
AGAGATCTGG ACAGCATGGA TCCTGCAGTC TATTCTTTAA CTATTGTCTC TGGTCAGAAT 
GTGTGCCCCA GTAATAGCAT GGGAAGCCCG TGCATTGAAG TCGACGTCCT GGGCATGCCT 
CTGGACAGCT GCCATTTCCG CACAAAGCCC ATCCATCGAA ACACCCTGAA CCCCATGTGG 
AACGAGCAGT TTCTGTTCCA CGTTCACTTC GAAGATCTTG TATTTCTTCG TTTTGCAGTT 
GTGGAAAACA ATAGTTCAGC GGTAACTGCT CAGAGAATCA TTCCACTGAA AGCTTTAAAA 
CGAGGATATC GACATCTTCA GCTGCGAAAC CTTCACAATG AAGTCTTGGA GATTTCTAGT 
TTATTCATTA ACAGCAGAAG GATGGAAGAA AATTCCTCTG GCAATACCAT GTCAGCCTCT 
TCGATGTTTA ATACAGAAGA AAGAAAATGT TTGCAGACTC ACAGAGTCAC GGTGCATGGG 
GTCCCAGGGC CAGAGCCCTT TACCGTTTTC ACTATTAATG GAGGCACCAA GGCAAAGCAG 
CTTCTGCAGC AAATTCTGAC AAATGAACAA GACATCAAAC CTGTTACCAC AGACTATTTT 
TTGATGGAAG AAAAATATTT TATATCTAAA GAAAAGAATG AATGTAGGAA ACAACCATTC 
CAGAGAGCCA TTGGTCCAGA AGAGGAGATC ATGCAAATTT TAAGCAGCTG GTTTCCAGAA 
GAGGGATACA TGGGCAGGAT TGTCTTAAAA ACCCAGCAGG AAAACCTAGA AGAGAAAAAC 
ATTGTTCAAG ATGACAAAGA GGTGATCTTG AGCTCAGAGG AGGAGAGTTT CTTTGTCCAA 
GTGCATGATG TTTCTCCAGA GCAACCTCGA ACAGTCATCA AAGCACCCCG CGTCAGCACT 
GCACAGGATG TCATTCAGCA GACCTTATGC AAAGCCAAAT ATTCCTACAG CATCCTGAGC 
AACCCCAATC CAAGCGACTA TGTGCTTTTG GAAGAGGTGG TGAAAGACAC TACCAACAAG 
AAGACTACCA CACCAAAGTC CTCTCAGCGG GTCCTTCTGG ATCAGGAGTG TGTGTTTCAA 
GCCCAAAGCA AGTGGAAAGG TGCAGGAAAA TTCATCCTTA AGCTAAAGGA GCAGGTGCAG 
GCATCTCGAG AAGATAAAAA GAAAGGCATT TCTTTCGCAA GTGAACTCAA GAAGCTCACC 
AAGTCAACTA AACAGCCCCG AGGACTTACA TCACCTTCTC AGCTCTTGAC CTCAGAAAGT 
ATCCAAACCA AGGAGGAGAA ACCTGTGGGT GGCTTGTCCT CCAGTGACAC AATGGATTAC 
CGACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( NM_001288989.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001275918.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant 3, mRNA.

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CloneID	OHu27664
Clone ID Related Accession (Same CDS sequence)	NM_001288989.2 , NM_001288989.1
Accession Version	NM_001288989.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL365510.32, BC151854.1, BC144286.1, AL139124.12 and AL355341.19. On Jun 2, 2019 this sequence version replaced NM_001288989.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.189769.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGTGCT TGGAGCAGTA GTAGCTGGCA CGGGCGGATC 
AAAGGCGGCA TGAAGGGATT TCAGAGCTTC ATGGTTTCAG ATAGCAACAT GAGTTTTGTT 
GAATTTGTTG AGCTGTTCAA ATCATTCAGT GTCAGGAGCC GCAAGGACCT GAAGGATCTG 
TTTGATGTCT ATGCAGTGCC CTGCAACCGA TCTGGCTCCG AGTCAGCCCC ACTCTACACC 
AACCTGACAA TTGATGAAAA CACCAGCGAT CTTCAGCCTG ACCTAGATCT GTTGACCAGA 
AATGTCTCGG ATTTGGGGTT GTTCATTAAG AGTAAACAGC AGCTATCGGA CAACCAGAGG 
CAGATATCTG ATGCCATTGC TGCTGCAAGC ATTGTGACAA ATGGCACTGG GATTGAGAGC 
ACATCTCTGG GCATTTTTGG GGTGGGCATA CTTCAGCTCA ACGATTTCCT CGTGAATTGC 
CAAGGAGAAC ACTGCACTTA TGATGAAATC CTCAGCATCA TCCAGAAGTT CGAGCCTAGC 
ATCAGTATGT GTCATCAGGG ACTAATGTCA TTTGAAGGGT TTGCCAGGTT TCTGATGGAT 
AAAGAAAATT TTGCCTCAAA AAATGATGAG TCACAGGAGA ACATTAAAGA ACTGCAGCTA 
CCCCTCTCAT ACTATTACAT CGAATCTTCG CACAATACCT ACCTCACGGG CCATCAGCTC 
AAAGGAGAAT CCTCGGTAGA ACTCTACAGC CAGGTCCTTT TGCAAGGCTG TCGAAGTGTA 
GAATTGGACT GCTGGGACGG AGACGATGGG ATGCCCATCA TTTATCATGG ACATACGCTG 
ACAACCAAGA TCCCCTTCAA GGAAGTGGTT GAAGCCATTG ATCGCAGTGC CTTCATCAAC 
TCTGACCTGC CAATCATCAT ATCGATTGAG AACCACTGTT CATTGCCTCA GCAACGAAAA 
ATGGCAGAAA TTTTCAAGAC TGTGTTTGGA GAAAAGCTGG TGACTAAATT CTTATTTGAG 
ACTGATTTCT CAGATGATCC AATGCTTCCT TCACCTGACC AACTCAGAAA GAAAGTTCTT 
CTTAAAAACA AGAAGCTAAA AGCCCATCAG ACGCCAGTGG ATATCTTAAA GCAAAAGGCT 
CATCAGTTAG CATCTATGCA AGTGCAGGCT TATAATGGTG GGAATGCCAA CCCCCGACCT 
GCCAATAATG AGGAAGAGGA AGATGAGGAG GACGAATATG ATTATGACTA TGAATCCCTT 
TCTGATGACA ACATTCTGGA AGACAGACCT GAAAATAAAT CATGTAATGA CAAGCTTCAG 
TTTGAATATA ATGAAGAAAT CCCAAAGAGG ATAAAGAAAG CAGATAACTC TGCTTGCAAC 
AAAGGAAAGG TTTATGATAT GGAACTGGGA GAAGAATTTT ATCTTGATCA GAATAAAAAG 
GAAAGCAGAC AGATTGCACC AGAGCTTTCT GACCTTGTAA TCTATTGTCA AGCAGTAAAA 
TTTCCAGGAC TGTCAACTCT AAATGCATCT GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC 
AGGAAGTCCA TTTTTGGCAA CAATCCGGGC AGAATGAGCC CAGGGGAGAC AGCATCATTT 
AACAAAACAT CTGGAAAAAG TTCCTGTGAA GGCATTCGAC AGACCTGGGA GGAATCTTCT 
TCCCCTCTCA ACCCAACCAC GTCCCTCAGT GCTATCATTA GAACTCCCAA ATGTTATCAT 
ATCTCGTCGC TGAATGAAAA TGCCGCCAAA CGTCTGTGTC GCAGGTATTC TCAGAAACTG 
ACCCAGCACA CCGCCTGTCA GCTGCTGAGA ACTTACCCTG CTGCCACCCG CATCGACTCT 
TCCAACCCGA ACCCCCTCAT GTTCTGGCTC CATGGGATAC AGCTTGTGGC ACTCAACTAC 
CAGACTGATG ATCTCCCTTT ACATTTAAAT GCTGCAATGT TTGAGGCAAA TGGTGGTTGT 
GGTTATGTAT TGAAACCTCC AGTTCTGTGG GACAAGAACT GCCCCATGTA TCAGAAGTTT 
TCTCCACTAG AAAGAGATCT GGACAGCATG GATCCTGCAG TCTATTCTTT AACTATTGTC 
TCTGGTCAGA ATGTGTGCCC CAGTAATAGC ATGGGAAGCC CGTGCATTGA AGTCGACGTC 
CTGGGCATGC CTCTGGACAG CTGCCATTTC CGCACAAAGC CCATCCATCG AAACACCCTG 
AACCCCATGT GGAACGAGCA GTTTCTGTTC CACGTTCACT TCGAAGATCT TGTATTTCTT 
CGTTTTGCAG TTGTGGAAAA CAATAGTTCA GCGGTAACTG CTCAGAGAAT CATTCCACTG 
AAAGCTTTAA AACGAGGATA TCGACATCTT CAGCTGCGAA ACCTTCACAA TGAAGTCTTG 
GAGATTTCTA GTTTATTCAT TAACAGCAGA AGGATGGAAG AAAATTCCTC TGGCAATACC 
ATGTCAGCCT CTTCGATGTT TAATACAGAA GAAAGAAAAT GTTTGCAGAC TCACAGAGTC 
ACGGTGCATG GGGTCCCAGG GCCAGAGCCC TTTACCGTTT TCACTATTAA TGGAGGCACC 
AAGGCAAAGC AGCTTCTGCA GCAAATTCTG ACAAATGAAC AAGACATCAA ACCTGTTACC 
ACAGACTATT TTTTGATGGA AGAAAAATAT TTTATATCTA AAGAAAAGAA TGAATGTAGG 
AAACAACCAT TCCAGAGAGC CATTGGTCCA GAAGAGGAGA TCATGCAAAT TTTAAGCAGC 
TGGTTTCCAG AAGAGGGATA CATGGGCAGG ATTGTCTTAA AAACCCAGCA GGAAAACCTA 
GAAGAGAAAA ACATTGTTCA AGATGACAAA GAGGTGATCT TGAGCTCAGA GGAGGAGAGT 
TTCTTTGTCC AAGTGCATGA TGTTTCTCCA GAGCAACCTC GAACAGTCAT CAAAGCACCC 
CGCGTCAGCA CTGCACAGGA TGTCATTCAG CAGACCTTAT GCAAAGCCAA ATATTCCTAC 
AGCATCCTGA GCAACCCCAA TCCAAGCGAC TATGTGCTTT TGGAAGAGGT GGTGAAAGAC 
ACTACCAACA AGAAGACTAC CACACCAAAG TCCTCTCAGC GGGTCCTTCT GGATCAGGAG 
TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA GGTGCAGGAA AATTCATCCT TAAGCTAAAG 
GAGCAGGTGC AGGCATCTCG AGAAGATAAA AAGAAAGGCA TTTCTTTCGC AAGTGAACTC 
AAGAAGCTCA CCAAGTCAAC TAAACAGCCC CGAGGACTTA CATCACCTTC TCAGCTCTTG 
ACCTCAGAAA GTATCCAAAC CAAGGAGGAG AAACCTGTGG GTGGCTTGTC CTCCAGTGAC 
ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001275918.1
CDS	693..7553
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	NM_001288989.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001288989.2

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGTGCT TGGAGCAGTA GTAGCTGGCA CGGGCGGATC 
AAAGGCGGCA TGAAGGGATT TCAGAGCTTC ATGGTTTCAG ATAGCAACAT GAGTTTTGTT 
GAATTTGTTG AGCTGTTCAA ATCATTCAGT GTCAGGAGCC GCAAGGACCT GAAGGATCTG 
TTTGATGTCT ATGCAGTGCC CTGCAACCGA TCTGGCTCCG AGTCAGCCCC ACTCTACACC 
AACCTGACAA TTGATGAAAA CACCAGCGAT CTTCAGCCTG ACCTAGATCT GTTGACCAGA 
AATGTCTCGG ATTTGGGGTT GTTCATTAAG AGTAAACAGC AGCTATCGGA CAACCAGAGG 
CAGATATCTG ATGCCATTGC TGCTGCAAGC ATTGTGACAA ATGGCACTGG GATTGAGAGC 
ACATCTCTGG GCATTTTTGG GGTGGGCATA CTTCAGCTCA ACGATTTCCT CGTGAATTGC 
CAAGGAGAAC ACTGCACTTA TGATGAAATC CTCAGCATCA TCCAGAAGTT CGAGCCTAGC 
ATCAGTATGT GTCATCAGGG ACTAATGTCA TTTGAAGGGT TTGCCAGGTT TCTGATGGAT 
AAAGAAAATT TTGCCTCAAA AAATGATGAG TCACAGGAGA ACATTAAAGA ACTGCAGCTA 
CCCCTCTCAT ACTATTACAT CGAATCTTCG CACAATACCT ACCTCACGGG CCATCAGCTC 
AAAGGAGAAT CCTCGGTAGA ACTCTACAGC CAGGTCCTTT TGCAAGGCTG TCGAAGTGTA 
GAATTGGACT GCTGGGACGG AGACGATGGG ATGCCCATCA TTTATCATGG ACATACGCTG 
ACAACCAAGA TCCCCTTCAA GGAAGTGGTT GAAGCCATTG ATCGCAGTGC CTTCATCAAC 
TCTGACCTGC CAATCATCAT ATCGATTGAG AACCACTGTT CATTGCCTCA GCAACGAAAA 
ATGGCAGAAA TTTTCAAGAC TGTGTTTGGA GAAAAGCTGG TGACTAAATT CTTATTTGAG 
ACTGATTTCT CAGATGATCC AATGCTTCCT TCACCTGACC AACTCAGAAA GAAAGTTCTT 
CTTAAAAACA AGAAGCTAAA AGCCCATCAG ACGCCAGTGG ATATCTTAAA GCAAAAGGCT 
CATCAGTTAG CATCTATGCA AGTGCAGGCT TATAATGGTG GGAATGCCAA CCCCCGACCT 
GCCAATAATG AGGAAGAGGA AGATGAGGAG GACGAATATG ATTATGACTA TGAATCCCTT 
TCTGATGACA ACATTCTGGA AGACAGACCT GAAAATAAAT CATGTAATGA CAAGCTTCAG 
TTTGAATATA ATGAAGAAAT CCCAAAGAGG ATAAAGAAAG CAGATAACTC TGCTTGCAAC 
AAAGGAAAGG TTTATGATAT GGAACTGGGA GAAGAATTTT ATCTTGATCA GAATAAAAAG 
GAAAGCAGAC AGATTGCACC AGAGCTTTCT GACCTTGTAA TCTATTGTCA AGCAGTAAAA 
TTTCCAGGAC TGTCAACTCT AAATGCATCT GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC 
AGGAAGTCCA TTTTTGGCAA CAATCCGGGC AGAATGAGCC CAGGGGAGAC AGCATCATTT 
AACAAAACAT CTGGAAAAAG TTCCTGTGAA GGCATTCGAC AGACCTGGGA GGAATCTTCT 
TCCCCTCTCA ACCCAACCAC GTCCCTCAGT GCTATCATTA GAACTCCCAA ATGTTATCAT 
ATCTCGTCGC TGAATGAAAA TGCCGCCAAA CGTCTGTGTC GCAGGTATTC TCAGAAACTG 
ACCCAGCACA CCGCCTGTCA GCTGCTGAGA ACTTACCCTG CTGCCACCCG CATCGACTCT 
TCCAACCCGA ACCCCCTCAT GTTCTGGCTC CATGGGATAC AGCTTGTGGC ACTCAACTAC 
CAGACTGATG ATCTCCCTTT ACATTTAAAT GCTGCAATGT TTGAGGCAAA TGGTGGTTGT 
GGTTATGTAT TGAAACCTCC AGTTCTGTGG GACAAGAACT GCCCCATGTA TCAGAAGTTT 
TCTCCACTAG AAAGAGATCT GGACAGCATG GATCCTGCAG TCTATTCTTT AACTATTGTC 
TCTGGTCAGA ATGTGTGCCC CAGTAATAGC ATGGGAAGCC CGTGCATTGA AGTCGACGTC 
CTGGGCATGC CTCTGGACAG CTGCCATTTC CGCACAAAGC CCATCCATCG AAACACCCTG 
AACCCCATGT GGAACGAGCA GTTTCTGTTC CACGTTCACT TCGAAGATCT TGTATTTCTT 
CGTTTTGCAG TTGTGGAAAA CAATAGTTCA GCGGTAACTG CTCAGAGAAT CATTCCACTG 
AAAGCTTTAA AACGAGGATA TCGACATCTT CAGCTGCGAA ACCTTCACAA TGAAGTCTTG 
GAGATTTCTA GTTTATTCAT TAACAGCAGA AGGATGGAAG AAAATTCCTC TGGCAATACC 
ATGTCAGCCT CTTCGATGTT TAATACAGAA GAAAGAAAAT GTTTGCAGAC TCACAGAGTC 
ACGGTGCATG GGGTCCCAGG GCCAGAGCCC TTTACCGTTT TCACTATTAA TGGAGGCACC 
AAGGCAAAGC AGCTTCTGCA GCAAATTCTG ACAAATGAAC AAGACATCAA ACCTGTTACC 
ACAGACTATT TTTTGATGGA AGAAAAATAT TTTATATCTA AAGAAAAGAA TGAATGTAGG 
AAACAACCAT TCCAGAGAGC CATTGGTCCA GAAGAGGAGA TCATGCAAAT TTTAAGCAGC 
TGGTTTCCAG AAGAGGGATA CATGGGCAGG ATTGTCTTAA AAACCCAGCA GGAAAACCTA 
GAAGAGAAAA ACATTGTTCA AGATGACAAA GAGGTGATCT TGAGCTCAGA GGAGGAGAGT 
TTCTTTGTCC AAGTGCATGA TGTTTCTCCA GAGCAACCTC GAACAGTCAT CAAAGCACCC 
CGCGTCAGCA CTGCACAGGA TGTCATTCAG CAGACCTTAT GCAAAGCCAA ATATTCCTAC 
AGCATCCTGA GCAACCCCAA TCCAAGCGAC TATGTGCTTT TGGAAGAGGT GGTGAAAGAC 
ACTACCAACA AGAAGACTAC CACACCAAAG TCCTCTCAGC GGGTCCTTCT GGATCAGGAG 
TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA GGTGCAGGAA AATTCATCCT TAAGCTAAAG 
GAGCAGGTGC AGGCATCTCG AGAAGATAAA AAGAAAGGCA TTTCTTTCGC AAGTGAACTC 
AAGAAGCTCA CCAAGTCAAC TAAACAGCCC CGAGGACTTA CATCACCTTC TCAGCTCTTG 
ACCTCAGAAA GTATCCAAAC CAAGGAGGAG AAACCTGTGG GTGGCTTGTC CTCCAGTGAC 
ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( NM_001288989.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001275918.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant 3, mRNA.

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CloneID	OHu27664
Clone ID Related Accession (Same CDS sequence)	NM_001288989.2 , NM_001288989.1
Accession Version	NM_001288989.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC151854.1 and BC144286.1. On Jan 9, 2014 this sequence version replaced XM_005269881.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.189769.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGTGCT TGGAGCAGTA GTAGCTGGCA CGGGCGGATC 
AAAGGCGGCA TGAAGGGATT TCAGAGCTTC ATGGTTTCAG ATAGCAACAT GAGTTTTGTT 
GAATTTGTTG AGCTGTTCAA ATCATTCAGT GTCAGGAGCC GCAAGGACCT GAAGGATCTG 
TTTGATGTCT ATGCAGTGCC CTGCAACCGA TCTGGCTCCG AGTCAGCCCC ACTCTACACC 
AACCTGACAA TTGATGAAAA CACCAGCGAT CTTCAGCCTG ACCTAGATCT GTTGACCAGA 
AATGTCTCGG ATTTGGGGTT GTTCATTAAG AGTAAACAGC AGCTATCGGA CAACCAGAGG 
CAGATATCTG ATGCCATTGC TGCTGCAAGC ATTGTGACAA ATGGCACTGG GATTGAGAGC 
ACATCTCTGG GCATTTTTGG GGTGGGCATA CTTCAGCTCA ACGATTTCCT CGTGAATTGC 
CAAGGAGAAC ACTGCACTTA TGATGAAATC CTCAGCATCA TCCAGAAGTT CGAGCCTAGC 
ATCAGTATGT GTCATCAGGG ACTAATGTCA TTTGAAGGGT TTGCCAGGTT TCTGATGGAT 
AAAGAAAATT TTGCCTCAAA AAATGATGAG TCACAGGAGA ACATTAAAGA ACTGCAGCTA 
CCCCTCTCAT ACTATTACAT CGAATCTTCG CACAATACCT ACCTCACGGG CCATCAGCTC 
AAAGGAGAAT CCTCGGTAGA ACTCTACAGC CAGGTCCTTT TGCAAGGCTG TCGAAGTGTA 
GAATTGGACT GCTGGGACGG AGACGATGGG ATGCCCATCA TTTATCATGG ACATACGCTG 
ACAACCAAGA TCCCCTTCAA GGAAGTGGTT GAAGCCATTG ATCGCAGTGC CTTCATCAAC 
TCTGACCTGC CAATCATCAT ATCGATTGAG AACCACTGTT CATTGCCTCA GCAACGAAAA 
ATGGCAGAAA TTTTCAAGAC TGTGTTTGGA GAAAAGCTGG TGACTAAATT CTTATTTGAG 
ACTGATTTCT CAGATGATCC AATGCTTCCT TCACCTGACC AACTCAGAAA GAAAGTTCTT 
CTTAAAAACA AGAAGCTAAA AGCCCATCAG ACGCCAGTGG ATATCTTAAA GCAAAAGGCT 
CATCAGTTAG CATCTATGCA AGTGCAGGCT TATAATGGTG GGAATGCCAA CCCCCGACCT 
GCCAATAATG AGGAAGAGGA AGATGAGGAG GACGAATATG ATTATGACTA TGAATCCCTT 
TCTGATGACA ACATTCTGGA AGACAGACCT GAAAATAAAT CATGTAATGA CAAGCTTCAG 
TTTGAATATA ATGAAGAAAT CCCAAAGAGG ATAAAGAAAG CAGATAACTC TGCTTGCAAC 
AAAGGAAAGG TTTATGATAT GGAACTGGGA GAAGAATTTT ATCTTGATCA GAATAAAAAG 
GAAAGCAGAC AGATTGCACC AGAGCTTTCT GACCTTGTAA TCTATTGTCA AGCAGTAAAA 
TTTCCAGGAC TGTCAACTCT AAATGCATCT GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC 
AGGAAGTCCA TTTTTGGCAA CAATCCGGGC AGAATGAGCC CAGGGGAGAC AGCATCATTT 
AACAAAACAT CTGGAAAAAG TTCCTGTGAA GGCATTCGAC AGACCTGGGA GGAATCTTCT 
TCCCCTCTCA ACCCAACCAC GTCCCTCAGT GCTATCATTA GAACTCCCAA ATGTTATCAT 
ATCTCGTCGC TGAATGAAAA TGCCGCCAAA CGTCTGTGTC GCAGGTATTC TCAGAAACTG 
ACCCAGCACA CCGCCTGTCA GCTGCTGAGA ACTTACCCTG CTGCCACCCG CATCGACTCT 
TCCAACCCGA ACCCCCTCAT GTTCTGGCTC CATGGGATAC AGCTTGTGGC ACTCAACTAC 
CAGACTGATG ATCTCCCTTT ACATTTAAAT GCTGCAATGT TTGAGGCAAA TGGTGGTTGT 
GGTTATGTAT TGAAACCTCC AGTTCTGTGG GACAAGAACT GCCCCATGTA TCAGAAGTTT 
TCTCCACTAG AAAGAGATCT GGACAGCATG GATCCTGCAG TCTATTCTTT AACTATTGTC 
TCTGGTCAGA ATGTGTGCCC CAGTAATAGC ATGGGAAGCC CGTGCATTGA AGTCGACGTC 
CTGGGCATGC CTCTGGACAG CTGCCATTTC CGCACAAAGC CCATCCATCG AAACACCCTG 
AACCCCATGT GGAACGAGCA GTTTCTGTTC CACGTTCACT TCGAAGATCT TGTATTTCTT 
CGTTTTGCAG TTGTGGAAAA CAATAGTTCA GCGGTAACTG CTCAGAGAAT CATTCCACTG 
AAAGCTTTAA AACGAGGATA TCGACATCTT CAGCTGCGAA ACCTTCACAA TGAAGTCTTG 
GAGATTTCTA GTTTATTCAT TAACAGCAGA AGGATGGAAG AAAATTCCTC TGGCAATACC 
ATGTCAGCCT CTTCGATGTT TAATACAGAA GAAAGAAAAT GTTTGCAGAC TCACAGAGTC 
ACGGTGCATG GGGTCCCAGG GCCAGAGCCC TTTACCGTTT TCACTATTAA TGGAGGCACC 
AAGGCAAAGC AGCTTCTGCA GCAAATTCTG ACAAATGAAC AAGACATCAA ACCTGTTACC 
ACAGACTATT TTTTGATGGA AGAAAAATAT TTTATATCTA AAGAAAAGAA TGAATGTAGG 
AAACAACCAT TCCAGAGAGC CATTGGTCCA GAAGAGGAGA TCATGCAAAT TTTAAGCAGC 
TGGTTTCCAG AAGAGGGATA CATGGGCAGG ATTGTCTTAA AAACCCAGCA GGAAAACCTA 
GAAGAGAAAA ACATTGTTCA AGATGACAAA GAGGTGATCT TGAGCTCAGA GGAGGAGAGT 
TTCTTTGTCC AAGTGCATGA TGTTTCTCCA GAGCAACCTC GAACAGTCAT CAAAGCACCC 
CGCGTCAGCA CTGCACAGGA TGTCATTCAG CAGACCTTAT GCAAAGCCAA ATATTCCTAC 
AGCATCCTGA GCAACCCCAA TCCAAGCGAC TATGTGCTTT TGGAAGAGGT GGTGAAAGAC 
ACTACCAACA AGAAGACTAC CACACCAAAG TCCTCTCAGC GGGTCCTTCT GGATCAGGAG 
TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA GGTGCAGGAA AATTCATCCT TAAGCTAAAG 
GAGCAGGTGC AGGCATCTCG AGAAGATAAA AAGAAAGGCA TTTCTTTCGC AAGTGAACTC 
AAGAAGCTCA CCAAGTCAAC TAAACAGCCC CGAGGACTTA CATCACCTTC TCAGCTCTTG 
ACCTCAGAAA GTATCCAAAC CAAGGAGGAG AAACCTGTGG GTGGCTTGTC CTCCAGTGAC 
ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001275918.1
CDS	635..7495
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	NM_001288989.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001288989.1

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGTGCT TGGAGCAGTA GTAGCTGGCA CGGGCGGATC 
AAAGGCGGCA TGAAGGGATT TCAGAGCTTC ATGGTTTCAG ATAGCAACAT GAGTTTTGTT 
GAATTTGTTG AGCTGTTCAA ATCATTCAGT GTCAGGAGCC GCAAGGACCT GAAGGATCTG 
TTTGATGTCT ATGCAGTGCC CTGCAACCGA TCTGGCTCCG AGTCAGCCCC ACTCTACACC 
AACCTGACAA TTGATGAAAA CACCAGCGAT CTTCAGCCTG ACCTAGATCT GTTGACCAGA 
AATGTCTCGG ATTTGGGGTT GTTCATTAAG AGTAAACAGC AGCTATCGGA CAACCAGAGG 
CAGATATCTG ATGCCATTGC TGCTGCAAGC ATTGTGACAA ATGGCACTGG GATTGAGAGC 
ACATCTCTGG GCATTTTTGG GGTGGGCATA CTTCAGCTCA ACGATTTCCT CGTGAATTGC 
CAAGGAGAAC ACTGCACTTA TGATGAAATC CTCAGCATCA TCCAGAAGTT CGAGCCTAGC 
ATCAGTATGT GTCATCAGGG ACTAATGTCA TTTGAAGGGT TTGCCAGGTT TCTGATGGAT 
AAAGAAAATT TTGCCTCAAA AAATGATGAG TCACAGGAGA ACATTAAAGA ACTGCAGCTA 
CCCCTCTCAT ACTATTACAT CGAATCTTCG CACAATACCT ACCTCACGGG CCATCAGCTC 
AAAGGAGAAT CCTCGGTAGA ACTCTACAGC CAGGTCCTTT TGCAAGGCTG TCGAAGTGTA 
GAATTGGACT GCTGGGACGG AGACGATGGG ATGCCCATCA TTTATCATGG ACATACGCTG 
ACAACCAAGA TCCCCTTCAA GGAAGTGGTT GAAGCCATTG ATCGCAGTGC CTTCATCAAC 
TCTGACCTGC CAATCATCAT ATCGATTGAG AACCACTGTT CATTGCCTCA GCAACGAAAA 
ATGGCAGAAA TTTTCAAGAC TGTGTTTGGA GAAAAGCTGG TGACTAAATT CTTATTTGAG 
ACTGATTTCT CAGATGATCC AATGCTTCCT TCACCTGACC AACTCAGAAA GAAAGTTCTT 
CTTAAAAACA AGAAGCTAAA AGCCCATCAG ACGCCAGTGG ATATCTTAAA GCAAAAGGCT 
CATCAGTTAG CATCTATGCA AGTGCAGGCT TATAATGGTG GGAATGCCAA CCCCCGACCT 
GCCAATAATG AGGAAGAGGA AGATGAGGAG GACGAATATG ATTATGACTA TGAATCCCTT 
TCTGATGACA ACATTCTGGA AGACAGACCT GAAAATAAAT CATGTAATGA CAAGCTTCAG 
TTTGAATATA ATGAAGAAAT CCCAAAGAGG ATAAAGAAAG CAGATAACTC TGCTTGCAAC 
AAAGGAAAGG TTTATGATAT GGAACTGGGA GAAGAATTTT ATCTTGATCA GAATAAAAAG 
GAAAGCAGAC AGATTGCACC AGAGCTTTCT GACCTTGTAA TCTATTGTCA AGCAGTAAAA 
TTTCCAGGAC TGTCAACTCT AAATGCATCT GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC 
AGGAAGTCCA TTTTTGGCAA CAATCCGGGC AGAATGAGCC CAGGGGAGAC AGCATCATTT 
AACAAAACAT CTGGAAAAAG TTCCTGTGAA GGCATTCGAC AGACCTGGGA GGAATCTTCT 
TCCCCTCTCA ACCCAACCAC GTCCCTCAGT GCTATCATTA GAACTCCCAA ATGTTATCAT 
ATCTCGTCGC TGAATGAAAA TGCCGCCAAA CGTCTGTGTC GCAGGTATTC TCAGAAACTG 
ACCCAGCACA CCGCCTGTCA GCTGCTGAGA ACTTACCCTG CTGCCACCCG CATCGACTCT 
TCCAACCCGA ACCCCCTCAT GTTCTGGCTC CATGGGATAC AGCTTGTGGC ACTCAACTAC 
CAGACTGATG ATCTCCCTTT ACATTTAAAT GCTGCAATGT TTGAGGCAAA TGGTGGTTGT 
GGTTATGTAT TGAAACCTCC AGTTCTGTGG GACAAGAACT GCCCCATGTA TCAGAAGTTT 
TCTCCACTAG AAAGAGATCT GGACAGCATG GATCCTGCAG TCTATTCTTT AACTATTGTC 
TCTGGTCAGA ATGTGTGCCC CAGTAATAGC ATGGGAAGCC CGTGCATTGA AGTCGACGTC 
CTGGGCATGC CTCTGGACAG CTGCCATTTC CGCACAAAGC CCATCCATCG AAACACCCTG 
AACCCCATGT GGAACGAGCA GTTTCTGTTC CACGTTCACT TCGAAGATCT TGTATTTCTT 
CGTTTTGCAG TTGTGGAAAA CAATAGTTCA GCGGTAACTG CTCAGAGAAT CATTCCACTG 
AAAGCTTTAA AACGAGGATA TCGACATCTT CAGCTGCGAA ACCTTCACAA TGAAGTCTTG 
GAGATTTCTA GTTTATTCAT TAACAGCAGA AGGATGGAAG AAAATTCCTC TGGCAATACC 
ATGTCAGCCT CTTCGATGTT TAATACAGAA GAAAGAAAAT GTTTGCAGAC TCACAGAGTC 
ACGGTGCATG GGGTCCCAGG GCCAGAGCCC TTTACCGTTT TCACTATTAA TGGAGGCACC 
AAGGCAAAGC AGCTTCTGCA GCAAATTCTG ACAAATGAAC AAGACATCAA ACCTGTTACC 
ACAGACTATT TTTTGATGGA AGAAAAATAT TTTATATCTA AAGAAAAGAA TGAATGTAGG 
AAACAACCAT TCCAGAGAGC CATTGGTCCA GAAGAGGAGA TCATGCAAAT TTTAAGCAGC 
TGGTTTCCAG AAGAGGGATA CATGGGCAGG ATTGTCTTAA AAACCCAGCA GGAAAACCTA 
GAAGAGAAAA ACATTGTTCA AGATGACAAA GAGGTGATCT TGAGCTCAGA GGAGGAGAGT 
TTCTTTGTCC AAGTGCATGA TGTTTCTCCA GAGCAACCTC GAACAGTCAT CAAAGCACCC 
CGCGTCAGCA CTGCACAGGA TGTCATTCAG CAGACCTTAT GCAAAGCCAA ATATTCCTAC 
AGCATCCTGA GCAACCCCAA TCCAAGCGAC TATGTGCTTT TGGAAGAGGT GGTGAAAGAC 
ACTACCAACA AGAAGACTAC CACACCAAAG TCCTCTCAGC GGGTCCTTCT GGATCAGGAG 
TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA GGTGCAGGAA AATTCATCCT TAAGCTAAAG 
GAGCAGGTGC AGGCATCTCG AGAAGATAAA AAGAAAGGCA TTTCTTTCGC AAGTGAACTC 
AAGAAGCTCA CCAAGTCAAC TAAACAGCCC CGAGGACTTA CATCACCTTC TCAGCTCTTG 
ACCTCAGAAA GTATCCAAAC CAAGGAGGAG AAACCTGTGG GTGGCTTGTC CTCCAGTGAC 
ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_006717885.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717948.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X1, mRNA.

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CloneID	OHu44365
Clone ID Related Accession (Same CDS sequence)	XM_006717885.3 , XM_017016310.1 , XM_017016311.2 , XM_006717885.4 , XM_017016310.2 , XM_011539849.2 , XM_011539849.3 , XM_017016311.1
Accession Version	XM_006717885.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717885.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAAAATAAAT CATGTAATGA CAAGCTTCAG TTTGAATATA ATGAAGAAAT CCCAAAGAGG 
ATAAAGAAAG CAGATAACTC TGCTTGCAAC AAAGGAAAGG TTTATGATAT GGAACTGGGA 
GAAGAATTTT ATCTTGATCA GAATAAAAAG GAAAGCAGAC AGATTGCACC AGAGCTTTCT 
GACCTTGTAA TCTATTGTCA AGCAGTAAAA TTTCCAGGAC TGTCAACTCT AAATGCATCT 
GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCGGGC 
AGAATGAGCC CAGGGGAGAC AGCATCATTT AACAAAACAT CTGGAAAAAG TTCCTGTGAA 
GGCATTCGAC AGACCTGGGA GGAATCTTCT TCCCCTCTCA ACCCAACCAC GTCCCTCAGT 
GCTATCATTA GAACTCCCAA ATGTTATCAT ATCTCGTCGC TGAATGAAAA TGCCGCCAAA 
CGTCTGTGTC GCAGGTATTC TCAGAAACTG ACCCAGCACA CCGCCTGTCA GCTGCTGAGA 
ACTTACCCTG CTGCCACCCG CATCGACTCT TCCAACCCGA ACCCCCTCAT GTTCTGGCTC 
CATGGGATAC AGCTTGTGGC ACTCAACTAC CAGACTGATG ATCTCCCTTT ACATTTAAAT 
GCTGCAATGT TTGAGGCAAA TGGTGGTTGT GGTTATGTAT TGAAACCTCC AGTTCTGTGG 
GACAAGAACT GCCCCATGTA TCAGAAGTTT TCTCCACTAG AAAGAGATCT GGACAGCATG 
GATCCTGCAG TCTATTCTTT AACTATTGTC TCTGGTCAGA ATGTGTGCCC CAGTAATAGC 
ATGGGAAGCC CGTGCATTGA AGTCGACGTC CTGGGCATGC CTCTGGACAG CTGCCATTTC 
CGCACAAAGC CCATCCATCG AAACACCCTG AACCCCATGT GGAACGAGCA GTTTCTGTTC 
CACGTTCACT TCGAAGATCT TGTATTTCTT CGTTTTGCAG TTGTGGAAAA CAATAGTTCA 
GCGGTAACTG CTCAGAGAAT CATTCCACTG AAAGCTTTAA AACGAGGATA TCGACATCTT 
CAGCTGCGAA ACCTTCACAA TGAAGTCTTG GAGATTTCTA GTTTATTCAT TAACAGCAGA 
AGGATGGAAG AAAATTCCTC TGGCAATACC ATGTCAGCCT CTTCGATGTT TAATACAGAA 
GAAAGAAAAT GTTTGCAGAC TCACAGAGTC ACGGTGCATG GGGTCCCAGG GCCAGAGCCC 
TTTACCGTTT TCACTATTAA TGGAGGCACC AAGGCAAAGC AGCTTCTGCA GCAAATTCTG 
ACAAATGAAC AAGACATCAA ACCTGTTACC ACAGACTATT TTTTGATGGA AGAAAAATAT 
TTTATATCTA AAGAAAAGAA TGAATGTAGG AAACAACCAT TCCAGAGAGC CATTGGTCCA 
GAAGAGGAGA TCATGCAAAT TTTAAGCAGC TGGTTTCCAG AAGAGGGATA CATGGGCAGG 
ATTGTCTTAA AAACCCAGCA GGAAAACCTA GAAGAGAAAA ACATTGTTCA AGATGACAAA 
GAGGTGATCT TGAGCTCAGA GGAGGAGAGT TTCTTTGTCC AAGTGCATGA TGTTTCTCCA 
GAGCAACCTC GAACAGTCAT CAAAGCACCC CGCGTCAGCA CTGCACAGGA TGTCATTCAG 
CAGACCTTAT GCAAAGCCAA ATATTCCTAC AGCATCCTGA GCAACCCCAA TCCAAGCGAC 
TATGTGCTTT TGGAAGAGGT GGTGAAAGAC ACTACCAACA AGAAGACTAC CACACCAAAG 
TCCTCTCAGC GGGTCCTTCT GGATCAGGAG TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA 
GGTGCAGGAA AATTCATCCT TAAGCTAAAG GAGCAGGTGC AGGCATCTCG AGAAGATAAA 
AAGAAAGGCA TTTCTTTCGC AAGTGAACTC AAGAAGCTCA CCAAGTCAAC TAAACAGCCC 
CGAGGACTTA CATCACCTTC TCAGCTCTTG ACCTCAGAAA GTATCCAAAC CAAGGAGGAG 
AAACCTGTGG GTGGCTTGTC CTCCAGTGAC ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717948.1
CDS	583..7533
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDADVLTASPAPNGQEDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_006717885.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717885.3

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAAAATAAAT CATGTAATGA CAAGCTTCAG TTTGAATATA ATGAAGAAAT CCCAAAGAGG 
ATAAAGAAAG CAGATAACTC TGCTTGCAAC AAAGGAAAGG TTTATGATAT GGAACTGGGA 
GAAGAATTTT ATCTTGATCA GAATAAAAAG GAAAGCAGAC AGATTGCACC AGAGCTTTCT 
GACCTTGTAA TCTATTGTCA AGCAGTAAAA TTTCCAGGAC TGTCAACTCT AAATGCATCT 
GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCGGGC 
AGAATGAGCC CAGGGGAGAC AGCATCATTT AACAAAACAT CTGGAAAAAG TTCCTGTGAA 
GGCATTCGAC AGACCTGGGA GGAATCTTCT TCCCCTCTCA ACCCAACCAC GTCCCTCAGT 
GCTATCATTA GAACTCCCAA ATGTTATCAT ATCTCGTCGC TGAATGAAAA TGCCGCCAAA 
CGTCTGTGTC GCAGGTATTC TCAGAAACTG ACCCAGCACA CCGCCTGTCA GCTGCTGAGA 
ACTTACCCTG CTGCCACCCG CATCGACTCT TCCAACCCGA ACCCCCTCAT GTTCTGGCTC 
CATGGGATAC AGCTTGTGGC ACTCAACTAC CAGACTGATG ATCTCCCTTT ACATTTAAAT 
GCTGCAATGT TTGAGGCAAA TGGTGGTTGT GGTTATGTAT TGAAACCTCC AGTTCTGTGG 
GACAAGAACT GCCCCATGTA TCAGAAGTTT TCTCCACTAG AAAGAGATCT GGACAGCATG 
GATCCTGCAG TCTATTCTTT AACTATTGTC TCTGGTCAGA ATGTGTGCCC CAGTAATAGC 
ATGGGAAGCC CGTGCATTGA AGTCGACGTC CTGGGCATGC CTCTGGACAG CTGCCATTTC 
CGCACAAAGC CCATCCATCG AAACACCCTG AACCCCATGT GGAACGAGCA GTTTCTGTTC 
CACGTTCACT TCGAAGATCT TGTATTTCTT CGTTTTGCAG TTGTGGAAAA CAATAGTTCA 
GCGGTAACTG CTCAGAGAAT CATTCCACTG AAAGCTTTAA AACGAGGATA TCGACATCTT 
CAGCTGCGAA ACCTTCACAA TGAAGTCTTG GAGATTTCTA GTTTATTCAT TAACAGCAGA 
AGGATGGAAG AAAATTCCTC TGGCAATACC ATGTCAGCCT CTTCGATGTT TAATACAGAA 
GAAAGAAAAT GTTTGCAGAC TCACAGAGTC ACGGTGCATG GGGTCCCAGG GCCAGAGCCC 
TTTACCGTTT TCACTATTAA TGGAGGCACC AAGGCAAAGC AGCTTCTGCA GCAAATTCTG 
ACAAATGAAC AAGACATCAA ACCTGTTACC ACAGACTATT TTTTGATGGA AGAAAAATAT 
TTTATATCTA AAGAAAAGAA TGAATGTAGG AAACAACCAT TCCAGAGAGC CATTGGTCCA 
GAAGAGGAGA TCATGCAAAT TTTAAGCAGC TGGTTTCCAG AAGAGGGATA CATGGGCAGG 
ATTGTCTTAA AAACCCAGCA GGAAAACCTA GAAGAGAAAA ACATTGTTCA AGATGACAAA 
GAGGTGATCT TGAGCTCAGA GGAGGAGAGT TTCTTTGTCC AAGTGCATGA TGTTTCTCCA 
GAGCAACCTC GAACAGTCAT CAAAGCACCC CGCGTCAGCA CTGCACAGGA TGTCATTCAG 
CAGACCTTAT GCAAAGCCAA ATATTCCTAC AGCATCCTGA GCAACCCCAA TCCAAGCGAC 
TATGTGCTTT TGGAAGAGGT GGTGAAAGAC ACTACCAACA AGAAGACTAC CACACCAAAG 
TCCTCTCAGC GGGTCCTTCT GGATCAGGAG TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA 
GGTGCAGGAA AATTCATCCT TAAGCTAAAG GAGCAGGTGC AGGCATCTCG AGAAGATAAA 
AAGAAAGGCA TTTCTTTCGC AAGTGAACTC AAGAAGCTCA CCAAGTCAAC TAAACAGCCC 
CGAGGACTTA CATCACCTTC TCAGCTCTTG ACCTCAGAAA GTATCCAAAC CAAGGAGGAG 
AAACCTGTGG GTGGCTTGTC CTCCAGTGAC ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_017016310.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871799.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X3, mRNA.

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CloneID	OHu44365
Clone ID Related Accession (Same CDS sequence)	XM_006717885.3 , XM_017016310.1 , XM_017016311.2 , XM_006717885.4 , XM_017016310.2 , XM_011539849.2 , XM_011539849.3 , XM_017016311.1
Accession Version	XM_017016310.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAAAATAAAT CATGTAATGA CAAGCTTCAG TTTGAATATA ATGAAGAAAT CCCAAAGAGG 
ATAAAGAAAG CAGATAACTC TGCTTGCAAC AAAGGAAAGG TTTATGATAT GGAACTGGGA 
GAAGAATTTT ATCTTGATCA GAATAAAAAG GAAAGCAGAC AGATTGCACC AGAGCTTTCT 
GACCTTGTAA TCTATTGTCA AGCAGTAAAA TTTCCAGGAC TGTCAACTCT AAATGCATCT 
GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCGGGC 
AGAATGAGCC CAGGGGAGAC AGCATCATTT AACAAAACAT CTGGAAAAAG TTCCTGTGAA 
GGCATTCGAC AGACCTGGGA GGAATCTTCT TCCCCTCTCA ACCCAACCAC GTCCCTCAGT 
GCTATCATTA GAACTCCCAA ATGTTATCAT ATCTCGTCGC TGAATGAAAA TGCCGCCAAA 
CGTCTGTGTC GCAGGTATTC TCAGAAACTG ACCCAGCACA CCGCCTGTCA GCTGCTGAGA 
ACTTACCCTG CTGCCACCCG CATCGACTCT TCCAACCCGA ACCCCCTCAT GTTCTGGCTC 
CATGGGATAC AGCTTGTGGC ACTCAACTAC CAGACTGATG ATCTCCCTTT ACATTTAAAT 
GCTGCAATGT TTGAGGCAAA TGGTGGTTGT GGTTATGTAT TGAAACCTCC AGTTCTGTGG 
GACAAGAACT GCCCCATGTA TCAGAAGTTT TCTCCACTAG AAAGAGATCT GGACAGCATG 
GATCCTGCAG TCTATTCTTT AACTATTGTC TCTGGTCAGA ATGTGTGCCC CAGTAATAGC 
ATGGGAAGCC CGTGCATTGA AGTCGACGTC CTGGGCATGC CTCTGGACAG CTGCCATTTC 
CGCACAAAGC CCATCCATCG AAACACCCTG AACCCCATGT GGAACGAGCA GTTTCTGTTC 
CACGTTCACT TCGAAGATCT TGTATTTCTT CGTTTTGCAG TTGTGGAAAA CAATAGTTCA 
GCGGTAACTG CTCAGAGAAT CATTCCACTG AAAGCTTTAA AACGAGGATA TCGACATCTT 
CAGCTGCGAA ACCTTCACAA TGAAGTCTTG GAGATTTCTA GTTTATTCAT TAACAGCAGA 
AGGATGGAAG AAAATTCCTC TGGCAATACC ATGTCAGCCT CTTCGATGTT TAATACAGAA 
GAAAGAAAAT GTTTGCAGAC TCACAGAGTC ACGGTGCATG GGGTCCCAGG GCCAGAGCCC 
TTTACCGTTT TCACTATTAA TGGAGGCACC AAGGCAAAGC AGCTTCTGCA GCAAATTCTG 
ACAAATGAAC AAGACATCAA ACCTGTTACC ACAGACTATT TTTTGATGGA AGAAAAATAT 
TTTATATCTA AAGAAAAGAA TGAATGTAGG AAACAACCAT TCCAGAGAGC CATTGGTCCA 
GAAGAGGAGA TCATGCAAAT TTTAAGCAGC TGGTTTCCAG AAGAGGGATA CATGGGCAGG 
ATTGTCTTAA AAACCCAGCA GGAAAACCTA GAAGAGAAAA ACATTGTTCA AGATGACAAA 
GAGGTGATCT TGAGCTCAGA GGAGGAGAGT TTCTTTGTCC AAGTGCATGA TGTTTCTCCA 
GAGCAACCTC GAACAGTCAT CAAAGCACCC CGCGTCAGCA CTGCACAGGA TGTCATTCAG 
CAGACCTTAT GCAAAGCCAA ATATTCCTAC AGCATCCTGA GCAACCCCAA TCCAAGCGAC 
TATGTGCTTT TGGAAGAGGT GGTGAAAGAC ACTACCAACA AGAAGACTAC CACACCAAAG 
TCCTCTCAGC GGGTCCTTCT GGATCAGGAG TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA 
GGTGCAGGAA AATTCATCCT TAAGCTAAAG GAGCAGGTGC AGGCATCTCG AGAAGATAAA 
AAGAAAGGCA TTTCTTTCGC AAGTGAACTC AAGAAGCTCA CCAAGTCAAC TAAACAGCCC 
CGAGGACTTA CATCACCTTC TCAGCTCTTG ACCTCAGAAA GTATCCAAAC CAAGGAGGAG 
AAACCTGTGG GTGGCTTGTC CTCCAGTGAC ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871799.1
CDS	630..7580
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDADVLTASPAPNGQEDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_017016310.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016310.1

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAAAATAAAT CATGTAATGA CAAGCTTCAG TTTGAATATA ATGAAGAAAT CCCAAAGAGG 
ATAAAGAAAG CAGATAACTC TGCTTGCAAC AAAGGAAAGG TTTATGATAT GGAACTGGGA 
GAAGAATTTT ATCTTGATCA GAATAAAAAG GAAAGCAGAC AGATTGCACC AGAGCTTTCT 
GACCTTGTAA TCTATTGTCA AGCAGTAAAA TTTCCAGGAC TGTCAACTCT AAATGCATCT 
GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCGGGC 
AGAATGAGCC CAGGGGAGAC AGCATCATTT AACAAAACAT CTGGAAAAAG TTCCTGTGAA 
GGCATTCGAC AGACCTGGGA GGAATCTTCT TCCCCTCTCA ACCCAACCAC GTCCCTCAGT 
GCTATCATTA GAACTCCCAA ATGTTATCAT ATCTCGTCGC TGAATGAAAA TGCCGCCAAA 
CGTCTGTGTC GCAGGTATTC TCAGAAACTG ACCCAGCACA CCGCCTGTCA GCTGCTGAGA 
ACTTACCCTG CTGCCACCCG CATCGACTCT TCCAACCCGA ACCCCCTCAT GTTCTGGCTC 
CATGGGATAC AGCTTGTGGC ACTCAACTAC CAGACTGATG ATCTCCCTTT ACATTTAAAT 
GCTGCAATGT TTGAGGCAAA TGGTGGTTGT GGTTATGTAT TGAAACCTCC AGTTCTGTGG 
GACAAGAACT GCCCCATGTA TCAGAAGTTT TCTCCACTAG AAAGAGATCT GGACAGCATG 
GATCCTGCAG TCTATTCTTT AACTATTGTC TCTGGTCAGA ATGTGTGCCC CAGTAATAGC 
ATGGGAAGCC CGTGCATTGA AGTCGACGTC CTGGGCATGC CTCTGGACAG CTGCCATTTC 
CGCACAAAGC CCATCCATCG AAACACCCTG AACCCCATGT GGAACGAGCA GTTTCTGTTC 
CACGTTCACT TCGAAGATCT TGTATTTCTT CGTTTTGCAG TTGTGGAAAA CAATAGTTCA 
GCGGTAACTG CTCAGAGAAT CATTCCACTG AAAGCTTTAA AACGAGGATA TCGACATCTT 
CAGCTGCGAA ACCTTCACAA TGAAGTCTTG GAGATTTCTA GTTTATTCAT TAACAGCAGA 
AGGATGGAAG AAAATTCCTC TGGCAATACC ATGTCAGCCT CTTCGATGTT TAATACAGAA 
GAAAGAAAAT GTTTGCAGAC TCACAGAGTC ACGGTGCATG GGGTCCCAGG GCCAGAGCCC 
TTTACCGTTT TCACTATTAA TGGAGGCACC AAGGCAAAGC AGCTTCTGCA GCAAATTCTG 
ACAAATGAAC AAGACATCAA ACCTGTTACC ACAGACTATT TTTTGATGGA AGAAAAATAT 
TTTATATCTA AAGAAAAGAA TGAATGTAGG AAACAACCAT TCCAGAGAGC CATTGGTCCA 
GAAGAGGAGA TCATGCAAAT TTTAAGCAGC TGGTTTCCAG AAGAGGGATA CATGGGCAGG 
ATTGTCTTAA AAACCCAGCA GGAAAACCTA GAAGAGAAAA ACATTGTTCA AGATGACAAA 
GAGGTGATCT TGAGCTCAGA GGAGGAGAGT TTCTTTGTCC AAGTGCATGA TGTTTCTCCA 
GAGCAACCTC GAACAGTCAT CAAAGCACCC CGCGTCAGCA CTGCACAGGA TGTCATTCAG 
CAGACCTTAT GCAAAGCCAA ATATTCCTAC AGCATCCTGA GCAACCCCAA TCCAAGCGAC 
TATGTGCTTT TGGAAGAGGT GGTGAAAGAC ACTACCAACA AGAAGACTAC CACACCAAAG 
TCCTCTCAGC GGGTCCTTCT GGATCAGGAG TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA 
GGTGCAGGAA AATTCATCCT TAAGCTAAAG GAGCAGGTGC AGGCATCTCG AGAAGATAAA 
AAGAAAGGCA TTTCTTTCGC AAGTGAACTC AAGAAGCTCA CCAAGTCAAC TAAACAGCCC 
CGAGGACTTA CATCACCTTC TCAGCTCTTG ACCTCAGAAA GTATCCAAAC CAAGGAGGAG 
AAACCTGTGG GTGGCTTGTC CTCCAGTGAC ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_017016311.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871800.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X4, mRNA.

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CloneID	OHu44365
Clone ID Related Accession (Same CDS sequence)	XM_006717885.3 , XM_017016310.1 , XM_017016311.2 , XM_006717885.4 , XM_017016310.2 , XM_011539849.2 , XM_011539849.3 , XM_017016311.1
Accession Version	XM_017016311.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016311.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAAAATAAAT CATGTAATGA CAAGCTTCAG TTTGAATATA ATGAAGAAAT CCCAAAGAGG 
ATAAAGAAAG CAGATAACTC TGCTTGCAAC AAAGGAAAGG TTTATGATAT GGAACTGGGA 
GAAGAATTTT ATCTTGATCA GAATAAAAAG GAAAGCAGAC AGATTGCACC AGAGCTTTCT 
GACCTTGTAA TCTATTGTCA AGCAGTAAAA TTTCCAGGAC TGTCAACTCT AAATGCATCT 
GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCGGGC 
AGAATGAGCC CAGGGGAGAC AGCATCATTT AACAAAACAT CTGGAAAAAG TTCCTGTGAA 
GGCATTCGAC AGACCTGGGA GGAATCTTCT TCCCCTCTCA ACCCAACCAC GTCCCTCAGT 
GCTATCATTA GAACTCCCAA ATGTTATCAT ATCTCGTCGC TGAATGAAAA TGCCGCCAAA 
CGTCTGTGTC GCAGGTATTC TCAGAAACTG ACCCAGCACA CCGCCTGTCA GCTGCTGAGA 
ACTTACCCTG CTGCCACCCG CATCGACTCT TCCAACCCGA ACCCCCTCAT GTTCTGGCTC 
CATGGGATAC AGCTTGTGGC ACTCAACTAC CAGACTGATG ATCTCCCTTT ACATTTAAAT 
GCTGCAATGT TTGAGGCAAA TGGTGGTTGT GGTTATGTAT TGAAACCTCC AGTTCTGTGG 
GACAAGAACT GCCCCATGTA TCAGAAGTTT TCTCCACTAG AAAGAGATCT GGACAGCATG 
GATCCTGCAG TCTATTCTTT AACTATTGTC TCTGGTCAGA ATGTGTGCCC CAGTAATAGC 
ATGGGAAGCC CGTGCATTGA AGTCGACGTC CTGGGCATGC CTCTGGACAG CTGCCATTTC 
CGCACAAAGC CCATCCATCG AAACACCCTG AACCCCATGT GGAACGAGCA GTTTCTGTTC 
CACGTTCACT TCGAAGATCT TGTATTTCTT CGTTTTGCAG TTGTGGAAAA CAATAGTTCA 
GCGGTAACTG CTCAGAGAAT CATTCCACTG AAAGCTTTAA AACGAGGATA TCGACATCTT 
CAGCTGCGAA ACCTTCACAA TGAAGTCTTG GAGATTTCTA GTTTATTCAT TAACAGCAGA 
AGGATGGAAG AAAATTCCTC TGGCAATACC ATGTCAGCCT CTTCGATGTT TAATACAGAA 
GAAAGAAAAT GTTTGCAGAC TCACAGAGTC ACGGTGCATG GGGTCCCAGG GCCAGAGCCC 
TTTACCGTTT TCACTATTAA TGGAGGCACC AAGGCAAAGC AGCTTCTGCA GCAAATTCTG 
ACAAATGAAC AAGACATCAA ACCTGTTACC ACAGACTATT TTTTGATGGA AGAAAAATAT 
TTTATATCTA AAGAAAAGAA TGAATGTAGG AAACAACCAT TCCAGAGAGC CATTGGTCCA 
GAAGAGGAGA TCATGCAAAT TTTAAGCAGC TGGTTTCCAG AAGAGGGATA CATGGGCAGG 
ATTGTCTTAA AAACCCAGCA GGAAAACCTA GAAGAGAAAA ACATTGTTCA AGATGACAAA 
GAGGTGATCT TGAGCTCAGA GGAGGAGAGT TTCTTTGTCC AAGTGCATGA TGTTTCTCCA 
GAGCAACCTC GAACAGTCAT CAAAGCACCC CGCGTCAGCA CTGCACAGGA TGTCATTCAG 
CAGACCTTAT GCAAAGCCAA ATATTCCTAC AGCATCCTGA GCAACCCCAA TCCAAGCGAC 
TATGTGCTTT TGGAAGAGGT GGTGAAAGAC ACTACCAACA AGAAGACTAC CACACCAAAG 
TCCTCTCAGC GGGTCCTTCT GGATCAGGAG TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA 
GGTGCAGGAA AATTCATCCT TAAGCTAAAG GAGCAGGTGC AGGCATCTCG AGAAGATAAA 
AAGAAAGGCA TTTCTTTCGC AAGTGAACTC AAGAAGCTCA CCAAGTCAAC TAAACAGCCC 
CGAGGACTTA CATCACCTTC TCAGCTCTTG ACCTCAGAAA GTATCCAAAC CAAGGAGGAG 
AAACCTGTGG GTGGCTTGTC CTCCAGTGAC ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871800.1
CDS	583..7533
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDADVLTASPAPNGQEDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_017016311.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016311.2

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAAAATAAAT CATGTAATGA CAAGCTTCAG TTTGAATATA ATGAAGAAAT CCCAAAGAGG 
ATAAAGAAAG CAGATAACTC TGCTTGCAAC AAAGGAAAGG TTTATGATAT GGAACTGGGA 
GAAGAATTTT ATCTTGATCA GAATAAAAAG GAAAGCAGAC AGATTGCACC AGAGCTTTCT 
GACCTTGTAA TCTATTGTCA AGCAGTAAAA TTTCCAGGAC TGTCAACTCT AAATGCATCT 
GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCGGGC 
AGAATGAGCC CAGGGGAGAC AGCATCATTT AACAAAACAT CTGGAAAAAG TTCCTGTGAA 
GGCATTCGAC AGACCTGGGA GGAATCTTCT TCCCCTCTCA ACCCAACCAC GTCCCTCAGT 
GCTATCATTA GAACTCCCAA ATGTTATCAT ATCTCGTCGC TGAATGAAAA TGCCGCCAAA 
CGTCTGTGTC GCAGGTATTC TCAGAAACTG ACCCAGCACA CCGCCTGTCA GCTGCTGAGA 
ACTTACCCTG CTGCCACCCG CATCGACTCT TCCAACCCGA ACCCCCTCAT GTTCTGGCTC 
CATGGGATAC AGCTTGTGGC ACTCAACTAC CAGACTGATG ATCTCCCTTT ACATTTAAAT 
GCTGCAATGT TTGAGGCAAA TGGTGGTTGT GGTTATGTAT TGAAACCTCC AGTTCTGTGG 
GACAAGAACT GCCCCATGTA TCAGAAGTTT TCTCCACTAG AAAGAGATCT GGACAGCATG 
GATCCTGCAG TCTATTCTTT AACTATTGTC TCTGGTCAGA ATGTGTGCCC CAGTAATAGC 
ATGGGAAGCC CGTGCATTGA AGTCGACGTC CTGGGCATGC CTCTGGACAG CTGCCATTTC 
CGCACAAAGC CCATCCATCG AAACACCCTG AACCCCATGT GGAACGAGCA GTTTCTGTTC 
CACGTTCACT TCGAAGATCT TGTATTTCTT CGTTTTGCAG TTGTGGAAAA CAATAGTTCA 
GCGGTAACTG CTCAGAGAAT CATTCCACTG AAAGCTTTAA AACGAGGATA TCGACATCTT 
CAGCTGCGAA ACCTTCACAA TGAAGTCTTG GAGATTTCTA GTTTATTCAT TAACAGCAGA 
AGGATGGAAG AAAATTCCTC TGGCAATACC ATGTCAGCCT CTTCGATGTT TAATACAGAA 
GAAAGAAAAT GTTTGCAGAC TCACAGAGTC ACGGTGCATG GGGTCCCAGG GCCAGAGCCC 
TTTACCGTTT TCACTATTAA TGGAGGCACC AAGGCAAAGC AGCTTCTGCA GCAAATTCTG 
ACAAATGAAC AAGACATCAA ACCTGTTACC ACAGACTATT TTTTGATGGA AGAAAAATAT 
TTTATATCTA AAGAAAAGAA TGAATGTAGG AAACAACCAT TCCAGAGAGC CATTGGTCCA 
GAAGAGGAGA TCATGCAAAT TTTAAGCAGC TGGTTTCCAG AAGAGGGATA CATGGGCAGG 
ATTGTCTTAA AAACCCAGCA GGAAAACCTA GAAGAGAAAA ACATTGTTCA AGATGACAAA 
GAGGTGATCT TGAGCTCAGA GGAGGAGAGT TTCTTTGTCC AAGTGCATGA TGTTTCTCCA 
GAGCAACCTC GAACAGTCAT CAAAGCACCC CGCGTCAGCA CTGCACAGGA TGTCATTCAG 
CAGACCTTAT GCAAAGCCAA ATATTCCTAC AGCATCCTGA GCAACCCCAA TCCAAGCGAC 
TATGTGCTTT TGGAAGAGGT GGTGAAAGAC ACTACCAACA AGAAGACTAC CACACCAAAG 
TCCTCTCAGC GGGTCCTTCT GGATCAGGAG TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA 
GGTGCAGGAA AATTCATCCT TAAGCTAAAG GAGCAGGTGC AGGCATCTCG AGAAGATAAA 
AAGAAAGGCA TTTCTTTCGC AAGTGAACTC AAGAAGCTCA CCAAGTCAAC TAAACAGCCC 
CGAGGACTTA CATCACCTTC TCAGCTCTTG ACCTCAGAAA GTATCCAAAC CAAGGAGGAG 
AAACCTGTGG GTGGCTTGTC CTCCAGTGAC ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_006717885.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717948.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X1, mRNA.

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CloneID	OHu44365
Clone ID Related Accession (Same CDS sequence)	XM_006717885.3 , XM_017016310.1 , XM_017016311.2 , XM_006717885.4 , XM_017016310.2 , XM_011539849.2 , XM_011539849.3 , XM_017016311.1
Accession Version	XM_006717885.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006717885.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAAAATAAAT CATGTAATGA CAAGCTTCAG TTTGAATATA ATGAAGAAAT CCCAAAGAGG 
ATAAAGAAAG CAGATAACTC TGCTTGCAAC AAAGGAAAGG TTTATGATAT GGAACTGGGA 
GAAGAATTTT ATCTTGATCA GAATAAAAAG GAAAGCAGAC AGATTGCACC AGAGCTTTCT 
GACCTTGTAA TCTATTGTCA AGCAGTAAAA TTTCCAGGAC TGTCAACTCT AAATGCATCT 
GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCGGGC 
AGAATGAGCC CAGGGGAGAC AGCATCATTT AACAAAACAT CTGGAAAAAG TTCCTGTGAA 
GGCATTCGAC AGACCTGGGA GGAATCTTCT TCCCCTCTCA ACCCAACCAC GTCCCTCAGT 
GCTATCATTA GAACTCCCAA ATGTTATCAT ATCTCGTCGC TGAATGAAAA TGCCGCCAAA 
CGTCTGTGTC GCAGGTATTC TCAGAAACTG ACCCAGCACA CCGCCTGTCA GCTGCTGAGA 
ACTTACCCTG CTGCCACCCG CATCGACTCT TCCAACCCGA ACCCCCTCAT GTTCTGGCTC 
CATGGGATAC AGCTTGTGGC ACTCAACTAC CAGACTGATG ATCTCCCTTT ACATTTAAAT 
GCTGCAATGT TTGAGGCAAA TGGTGGTTGT GGTTATGTAT TGAAACCTCC AGTTCTGTGG 
GACAAGAACT GCCCCATGTA TCAGAAGTTT TCTCCACTAG AAAGAGATCT GGACAGCATG 
GATCCTGCAG TCTATTCTTT AACTATTGTC TCTGGTCAGA ATGTGTGCCC CAGTAATAGC 
ATGGGAAGCC CGTGCATTGA AGTCGACGTC CTGGGCATGC CTCTGGACAG CTGCCATTTC 
CGCACAAAGC CCATCCATCG AAACACCCTG AACCCCATGT GGAACGAGCA GTTTCTGTTC 
CACGTTCACT TCGAAGATCT TGTATTTCTT CGTTTTGCAG TTGTGGAAAA CAATAGTTCA 
GCGGTAACTG CTCAGAGAAT CATTCCACTG AAAGCTTTAA AACGAGGATA TCGACATCTT 
CAGCTGCGAA ACCTTCACAA TGAAGTCTTG GAGATTTCTA GTTTATTCAT TAACAGCAGA 
AGGATGGAAG AAAATTCCTC TGGCAATACC ATGTCAGCCT CTTCGATGTT TAATACAGAA 
GAAAGAAAAT GTTTGCAGAC TCACAGAGTC ACGGTGCATG GGGTCCCAGG GCCAGAGCCC 
TTTACCGTTT TCACTATTAA TGGAGGCACC AAGGCAAAGC AGCTTCTGCA GCAAATTCTG 
ACAAATGAAC AAGACATCAA ACCTGTTACC ACAGACTATT TTTTGATGGA AGAAAAATAT 
TTTATATCTA AAGAAAAGAA TGAATGTAGG AAACAACCAT TCCAGAGAGC CATTGGTCCA 
GAAGAGGAGA TCATGCAAAT TTTAAGCAGC TGGTTTCCAG AAGAGGGATA CATGGGCAGG 
ATTGTCTTAA AAACCCAGCA GGAAAACCTA GAAGAGAAAA ACATTGTTCA AGATGACAAA 
GAGGTGATCT TGAGCTCAGA GGAGGAGAGT TTCTTTGTCC AAGTGCATGA TGTTTCTCCA 
GAGCAACCTC GAACAGTCAT CAAAGCACCC CGCGTCAGCA CTGCACAGGA TGTCATTCAG 
CAGACCTTAT GCAAAGCCAA ATATTCCTAC AGCATCCTGA GCAACCCCAA TCCAAGCGAC 
TATGTGCTTT TGGAAGAGGT GGTGAAAGAC ACTACCAACA AGAAGACTAC CACACCAAAG 
TCCTCTCAGC GGGTCCTTCT GGATCAGGAG TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA 
GGTGCAGGAA AATTCATCCT TAAGCTAAAG GAGCAGGTGC AGGCATCTCG AGAAGATAAA 
AAGAAAGGCA TTTCTTTCGC AAGTGAACTC AAGAAGCTCA CCAAGTCAAC TAAACAGCCC 
CGAGGACTTA CATCACCTTC TCAGCTCTTG ACCTCAGAAA GTATCCAAAC CAAGGAGGAG 
AAACCTGTGG GTGGCTTGTC CTCCAGTGAC ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717948.1
CDS	583..7533
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDADVLTASPAPNGQEDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_006717885.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717885.4

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAAAATAAAT CATGTAATGA CAAGCTTCAG TTTGAATATA ATGAAGAAAT CCCAAAGAGG 
ATAAAGAAAG CAGATAACTC TGCTTGCAAC AAAGGAAAGG TTTATGATAT GGAACTGGGA 
GAAGAATTTT ATCTTGATCA GAATAAAAAG GAAAGCAGAC AGATTGCACC AGAGCTTTCT 
GACCTTGTAA TCTATTGTCA AGCAGTAAAA TTTCCAGGAC TGTCAACTCT AAATGCATCT 
GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCGGGC 
AGAATGAGCC CAGGGGAGAC AGCATCATTT AACAAAACAT CTGGAAAAAG TTCCTGTGAA 
GGCATTCGAC AGACCTGGGA GGAATCTTCT TCCCCTCTCA ACCCAACCAC GTCCCTCAGT 
GCTATCATTA GAACTCCCAA ATGTTATCAT ATCTCGTCGC TGAATGAAAA TGCCGCCAAA 
CGTCTGTGTC GCAGGTATTC TCAGAAACTG ACCCAGCACA CCGCCTGTCA GCTGCTGAGA 
ACTTACCCTG CTGCCACCCG CATCGACTCT TCCAACCCGA ACCCCCTCAT GTTCTGGCTC 
CATGGGATAC AGCTTGTGGC ACTCAACTAC CAGACTGATG ATCTCCCTTT ACATTTAAAT 
GCTGCAATGT TTGAGGCAAA TGGTGGTTGT GGTTATGTAT TGAAACCTCC AGTTCTGTGG 
GACAAGAACT GCCCCATGTA TCAGAAGTTT TCTCCACTAG AAAGAGATCT GGACAGCATG 
GATCCTGCAG TCTATTCTTT AACTATTGTC TCTGGTCAGA ATGTGTGCCC CAGTAATAGC 
ATGGGAAGCC CGTGCATTGA AGTCGACGTC CTGGGCATGC CTCTGGACAG CTGCCATTTC 
CGCACAAAGC CCATCCATCG AAACACCCTG AACCCCATGT GGAACGAGCA GTTTCTGTTC 
CACGTTCACT TCGAAGATCT TGTATTTCTT CGTTTTGCAG TTGTGGAAAA CAATAGTTCA 
GCGGTAACTG CTCAGAGAAT CATTCCACTG AAAGCTTTAA AACGAGGATA TCGACATCTT 
CAGCTGCGAA ACCTTCACAA TGAAGTCTTG GAGATTTCTA GTTTATTCAT TAACAGCAGA 
AGGATGGAAG AAAATTCCTC TGGCAATACC ATGTCAGCCT CTTCGATGTT TAATACAGAA 
GAAAGAAAAT GTTTGCAGAC TCACAGAGTC ACGGTGCATG GGGTCCCAGG GCCAGAGCCC 
TTTACCGTTT TCACTATTAA TGGAGGCACC AAGGCAAAGC AGCTTCTGCA GCAAATTCTG 
ACAAATGAAC AAGACATCAA ACCTGTTACC ACAGACTATT TTTTGATGGA AGAAAAATAT 
TTTATATCTA AAGAAAAGAA TGAATGTAGG AAACAACCAT TCCAGAGAGC CATTGGTCCA 
GAAGAGGAGA TCATGCAAAT TTTAAGCAGC TGGTTTCCAG AAGAGGGATA CATGGGCAGG 
ATTGTCTTAA AAACCCAGCA GGAAAACCTA GAAGAGAAAA ACATTGTTCA AGATGACAAA 
GAGGTGATCT TGAGCTCAGA GGAGGAGAGT TTCTTTGTCC AAGTGCATGA TGTTTCTCCA 
GAGCAACCTC GAACAGTCAT CAAAGCACCC CGCGTCAGCA CTGCACAGGA TGTCATTCAG 
CAGACCTTAT GCAAAGCCAA ATATTCCTAC AGCATCCTGA GCAACCCCAA TCCAAGCGAC 
TATGTGCTTT TGGAAGAGGT GGTGAAAGAC ACTACCAACA AGAAGACTAC CACACCAAAG 
TCCTCTCAGC GGGTCCTTCT GGATCAGGAG TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA 
GGTGCAGGAA AATTCATCCT TAAGCTAAAG GAGCAGGTGC AGGCATCTCG AGAAGATAAA 
AAGAAAGGCA TTTCTTTCGC AAGTGAACTC AAGAAGCTCA CCAAGTCAAC TAAACAGCCC 
CGAGGACTTA CATCACCTTC TCAGCTCTTG ACCTCAGAAA GTATCCAAAC CAAGGAGGAG 
AAACCTGTGG GTGGCTTGTC CTCCAGTGAC ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_017016310.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871799.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X3, mRNA.

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CloneID	OHu44365
Clone ID Related Accession (Same CDS sequence)	XM_006717885.3 , XM_017016310.1 , XM_017016311.2 , XM_006717885.4 , XM_017016310.2 , XM_011539849.2 , XM_011539849.3 , XM_017016311.1
Accession Version	XM_017016310.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016310.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAAAATAAAT CATGTAATGA CAAGCTTCAG TTTGAATATA ATGAAGAAAT CCCAAAGAGG 
ATAAAGAAAG CAGATAACTC TGCTTGCAAC AAAGGAAAGG TTTATGATAT GGAACTGGGA 
GAAGAATTTT ATCTTGATCA GAATAAAAAG GAAAGCAGAC AGATTGCACC AGAGCTTTCT 
GACCTTGTAA TCTATTGTCA AGCAGTAAAA TTTCCAGGAC TGTCAACTCT AAATGCATCT 
GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCGGGC 
AGAATGAGCC CAGGGGAGAC AGCATCATTT AACAAAACAT CTGGAAAAAG TTCCTGTGAA 
GGCATTCGAC AGACCTGGGA GGAATCTTCT TCCCCTCTCA ACCCAACCAC GTCCCTCAGT 
GCTATCATTA GAACTCCCAA ATGTTATCAT ATCTCGTCGC TGAATGAAAA TGCCGCCAAA 
CGTCTGTGTC GCAGGTATTC TCAGAAACTG ACCCAGCACA CCGCCTGTCA GCTGCTGAGA 
ACTTACCCTG CTGCCACCCG CATCGACTCT TCCAACCCGA ACCCCCTCAT GTTCTGGCTC 
CATGGGATAC AGCTTGTGGC ACTCAACTAC CAGACTGATG ATCTCCCTTT ACATTTAAAT 
GCTGCAATGT TTGAGGCAAA TGGTGGTTGT GGTTATGTAT TGAAACCTCC AGTTCTGTGG 
GACAAGAACT GCCCCATGTA TCAGAAGTTT TCTCCACTAG AAAGAGATCT GGACAGCATG 
GATCCTGCAG TCTATTCTTT AACTATTGTC TCTGGTCAGA ATGTGTGCCC CAGTAATAGC 
ATGGGAAGCC CGTGCATTGA AGTCGACGTC CTGGGCATGC CTCTGGACAG CTGCCATTTC 
CGCACAAAGC CCATCCATCG AAACACCCTG AACCCCATGT GGAACGAGCA GTTTCTGTTC 
CACGTTCACT TCGAAGATCT TGTATTTCTT CGTTTTGCAG TTGTGGAAAA CAATAGTTCA 
GCGGTAACTG CTCAGAGAAT CATTCCACTG AAAGCTTTAA AACGAGGATA TCGACATCTT 
CAGCTGCGAA ACCTTCACAA TGAAGTCTTG GAGATTTCTA GTTTATTCAT TAACAGCAGA 
AGGATGGAAG AAAATTCCTC TGGCAATACC ATGTCAGCCT CTTCGATGTT TAATACAGAA 
GAAAGAAAAT GTTTGCAGAC TCACAGAGTC ACGGTGCATG GGGTCCCAGG GCCAGAGCCC 
TTTACCGTTT TCACTATTAA TGGAGGCACC AAGGCAAAGC AGCTTCTGCA GCAAATTCTG 
ACAAATGAAC AAGACATCAA ACCTGTTACC ACAGACTATT TTTTGATGGA AGAAAAATAT 
TTTATATCTA AAGAAAAGAA TGAATGTAGG AAACAACCAT TCCAGAGAGC CATTGGTCCA 
GAAGAGGAGA TCATGCAAAT TTTAAGCAGC TGGTTTCCAG AAGAGGGATA CATGGGCAGG 
ATTGTCTTAA AAACCCAGCA GGAAAACCTA GAAGAGAAAA ACATTGTTCA AGATGACAAA 
GAGGTGATCT TGAGCTCAGA GGAGGAGAGT TTCTTTGTCC AAGTGCATGA TGTTTCTCCA 
GAGCAACCTC GAACAGTCAT CAAAGCACCC CGCGTCAGCA CTGCACAGGA TGTCATTCAG 
CAGACCTTAT GCAAAGCCAA ATATTCCTAC AGCATCCTGA GCAACCCCAA TCCAAGCGAC 
TATGTGCTTT TGGAAGAGGT GGTGAAAGAC ACTACCAACA AGAAGACTAC CACACCAAAG 
TCCTCTCAGC GGGTCCTTCT GGATCAGGAG TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA 
GGTGCAGGAA AATTCATCCT TAAGCTAAAG GAGCAGGTGC AGGCATCTCG AGAAGATAAA 
AAGAAAGGCA TTTCTTTCGC AAGTGAACTC AAGAAGCTCA CCAAGTCAAC TAAACAGCCC 
CGAGGACTTA CATCACCTTC TCAGCTCTTG ACCTCAGAAA GTATCCAAAC CAAGGAGGAG 
AAACCTGTGG GTGGCTTGTC CTCCAGTGAC ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871799.1
CDS	630..7580
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDADVLTASPAPNGQEDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_017016310.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016310.2

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAAAATAAAT CATGTAATGA CAAGCTTCAG TTTGAATATA ATGAAGAAAT CCCAAAGAGG 
ATAAAGAAAG CAGATAACTC TGCTTGCAAC AAAGGAAAGG TTTATGATAT GGAACTGGGA 
GAAGAATTTT ATCTTGATCA GAATAAAAAG GAAAGCAGAC AGATTGCACC AGAGCTTTCT 
GACCTTGTAA TCTATTGTCA AGCAGTAAAA TTTCCAGGAC TGTCAACTCT AAATGCATCT 
GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCGGGC 
AGAATGAGCC CAGGGGAGAC AGCATCATTT AACAAAACAT CTGGAAAAAG TTCCTGTGAA 
GGCATTCGAC AGACCTGGGA GGAATCTTCT TCCCCTCTCA ACCCAACCAC GTCCCTCAGT 
GCTATCATTA GAACTCCCAA ATGTTATCAT ATCTCGTCGC TGAATGAAAA TGCCGCCAAA 
CGTCTGTGTC GCAGGTATTC TCAGAAACTG ACCCAGCACA CCGCCTGTCA GCTGCTGAGA 
ACTTACCCTG CTGCCACCCG CATCGACTCT TCCAACCCGA ACCCCCTCAT GTTCTGGCTC 
CATGGGATAC AGCTTGTGGC ACTCAACTAC CAGACTGATG ATCTCCCTTT ACATTTAAAT 
GCTGCAATGT TTGAGGCAAA TGGTGGTTGT GGTTATGTAT TGAAACCTCC AGTTCTGTGG 
GACAAGAACT GCCCCATGTA TCAGAAGTTT TCTCCACTAG AAAGAGATCT GGACAGCATG 
GATCCTGCAG TCTATTCTTT AACTATTGTC TCTGGTCAGA ATGTGTGCCC CAGTAATAGC 
ATGGGAAGCC CGTGCATTGA AGTCGACGTC CTGGGCATGC CTCTGGACAG CTGCCATTTC 
CGCACAAAGC CCATCCATCG AAACACCCTG AACCCCATGT GGAACGAGCA GTTTCTGTTC 
CACGTTCACT TCGAAGATCT TGTATTTCTT CGTTTTGCAG TTGTGGAAAA CAATAGTTCA 
GCGGTAACTG CTCAGAGAAT CATTCCACTG AAAGCTTTAA AACGAGGATA TCGACATCTT 
CAGCTGCGAA ACCTTCACAA TGAAGTCTTG GAGATTTCTA GTTTATTCAT TAACAGCAGA 
AGGATGGAAG AAAATTCCTC TGGCAATACC ATGTCAGCCT CTTCGATGTT TAATACAGAA 
GAAAGAAAAT GTTTGCAGAC TCACAGAGTC ACGGTGCATG GGGTCCCAGG GCCAGAGCCC 
TTTACCGTTT TCACTATTAA TGGAGGCACC AAGGCAAAGC AGCTTCTGCA GCAAATTCTG 
ACAAATGAAC AAGACATCAA ACCTGTTACC ACAGACTATT TTTTGATGGA AGAAAAATAT 
TTTATATCTA AAGAAAAGAA TGAATGTAGG AAACAACCAT TCCAGAGAGC CATTGGTCCA 
GAAGAGGAGA TCATGCAAAT TTTAAGCAGC TGGTTTCCAG AAGAGGGATA CATGGGCAGG 
ATTGTCTTAA AAACCCAGCA GGAAAACCTA GAAGAGAAAA ACATTGTTCA AGATGACAAA 
GAGGTGATCT TGAGCTCAGA GGAGGAGAGT TTCTTTGTCC AAGTGCATGA TGTTTCTCCA 
GAGCAACCTC GAACAGTCAT CAAAGCACCC CGCGTCAGCA CTGCACAGGA TGTCATTCAG 
CAGACCTTAT GCAAAGCCAA ATATTCCTAC AGCATCCTGA GCAACCCCAA TCCAAGCGAC 
TATGTGCTTT TGGAAGAGGT GGTGAAAGAC ACTACCAACA AGAAGACTAC CACACCAAAG 
TCCTCTCAGC GGGTCCTTCT GGATCAGGAG TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA 
GGTGCAGGAA AATTCATCCT TAAGCTAAAG GAGCAGGTGC AGGCATCTCG AGAAGATAAA 
AAGAAAGGCA TTTCTTTCGC AAGTGAACTC AAGAAGCTCA CCAAGTCAAC TAAACAGCCC 
CGAGGACTTA CATCACCTTC TCAGCTCTTG ACCTCAGAAA GTATCCAAAC CAAGGAGGAG 
AAACCTGTGG GTGGCTTGTC CTCCAGTGAC ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_011539849.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538151.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X2, mRNA.

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CloneID	OHu44365
Clone ID Related Accession (Same CDS sequence)	XM_006717885.3 , XM_017016310.1 , XM_017016311.2 , XM_006717885.4 , XM_017016310.2 , XM_011539849.2 , XM_011539849.3 , XM_017016311.1
Accession Version	XM_011539849.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539849.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAAAATAAAT CATGTAATGA CAAGCTTCAG TTTGAATATA ATGAAGAAAT CCCAAAGAGG 
ATAAAGAAAG CAGATAACTC TGCTTGCAAC AAAGGAAAGG TTTATGATAT GGAACTGGGA 
GAAGAATTTT ATCTTGATCA GAATAAAAAG GAAAGCAGAC AGATTGCACC AGAGCTTTCT 
GACCTTGTAA TCTATTGTCA AGCAGTAAAA TTTCCAGGAC TGTCAACTCT AAATGCATCT 
GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCGGGC 
AGAATGAGCC CAGGGGAGAC AGCATCATTT AACAAAACAT CTGGAAAAAG TTCCTGTGAA 
GGCATTCGAC AGACCTGGGA GGAATCTTCT TCCCCTCTCA ACCCAACCAC GTCCCTCAGT 
GCTATCATTA GAACTCCCAA ATGTTATCAT ATCTCGTCGC TGAATGAAAA TGCCGCCAAA 
CGTCTGTGTC GCAGGTATTC TCAGAAACTG ACCCAGCACA CCGCCTGTCA GCTGCTGAGA 
ACTTACCCTG CTGCCACCCG CATCGACTCT TCCAACCCGA ACCCCCTCAT GTTCTGGCTC 
CATGGGATAC AGCTTGTGGC ACTCAACTAC CAGACTGATG ATCTCCCTTT ACATTTAAAT 
GCTGCAATGT TTGAGGCAAA TGGTGGTTGT GGTTATGTAT TGAAACCTCC AGTTCTGTGG 
GACAAGAACT GCCCCATGTA TCAGAAGTTT TCTCCACTAG AAAGAGATCT GGACAGCATG 
GATCCTGCAG TCTATTCTTT AACTATTGTC TCTGGTCAGA ATGTGTGCCC CAGTAATAGC 
ATGGGAAGCC CGTGCATTGA AGTCGACGTC CTGGGCATGC CTCTGGACAG CTGCCATTTC 
CGCACAAAGC CCATCCATCG AAACACCCTG AACCCCATGT GGAACGAGCA GTTTCTGTTC 
CACGTTCACT TCGAAGATCT TGTATTTCTT CGTTTTGCAG TTGTGGAAAA CAATAGTTCA 
GCGGTAACTG CTCAGAGAAT CATTCCACTG AAAGCTTTAA AACGAGGATA TCGACATCTT 
CAGCTGCGAA ACCTTCACAA TGAAGTCTTG GAGATTTCTA GTTTATTCAT TAACAGCAGA 
AGGATGGAAG AAAATTCCTC TGGCAATACC ATGTCAGCCT CTTCGATGTT TAATACAGAA 
GAAAGAAAAT GTTTGCAGAC TCACAGAGTC ACGGTGCATG GGGTCCCAGG GCCAGAGCCC 
TTTACCGTTT TCACTATTAA TGGAGGCACC AAGGCAAAGC AGCTTCTGCA GCAAATTCTG 
ACAAATGAAC AAGACATCAA ACCTGTTACC ACAGACTATT TTTTGATGGA AGAAAAATAT 
TTTATATCTA AAGAAAAGAA TGAATGTAGG AAACAACCAT TCCAGAGAGC CATTGGTCCA 
GAAGAGGAGA TCATGCAAAT TTTAAGCAGC TGGTTTCCAG AAGAGGGATA CATGGGCAGG 
ATTGTCTTAA AAACCCAGCA GGAAAACCTA GAAGAGAAAA ACATTGTTCA AGATGACAAA 
GAGGTGATCT TGAGCTCAGA GGAGGAGAGT TTCTTTGTCC AAGTGCATGA TGTTTCTCCA 
GAGCAACCTC GAACAGTCAT CAAAGCACCC CGCGTCAGCA CTGCACAGGA TGTCATTCAG 
CAGACCTTAT GCAAAGCCAA ATATTCCTAC AGCATCCTGA GCAACCCCAA TCCAAGCGAC 
TATGTGCTTT TGGAAGAGGT GGTGAAAGAC ACTACCAACA AGAAGACTAC CACACCAAAG 
TCCTCTCAGC GGGTCCTTCT GGATCAGGAG TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA 
GGTGCAGGAA AATTCATCCT TAAGCTAAAG GAGCAGGTGC AGGCATCTCG AGAAGATAAA 
AAGAAAGGCA TTTCTTTCGC AAGTGAACTC AAGAAGCTCA CCAAGTCAAC TAAACAGCCC 
CGAGGACTTA CATCACCTTC TCAGCTCTTG ACCTCAGAAA GTATCCAAAC CAAGGAGGAG 
AAACCTGTGG GTGGCTTGTC CTCCAGTGAC ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538151.1
CDS	1740..8690
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDADVLTASPAPNGQEDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_011539849.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539849.2

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAAAATAAAT CATGTAATGA CAAGCTTCAG TTTGAATATA ATGAAGAAAT CCCAAAGAGG 
ATAAAGAAAG CAGATAACTC TGCTTGCAAC AAAGGAAAGG TTTATGATAT GGAACTGGGA 
GAAGAATTTT ATCTTGATCA GAATAAAAAG GAAAGCAGAC AGATTGCACC AGAGCTTTCT 
GACCTTGTAA TCTATTGTCA AGCAGTAAAA TTTCCAGGAC TGTCAACTCT AAATGCATCT 
GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCGGGC 
AGAATGAGCC CAGGGGAGAC AGCATCATTT AACAAAACAT CTGGAAAAAG TTCCTGTGAA 
GGCATTCGAC AGACCTGGGA GGAATCTTCT TCCCCTCTCA ACCCAACCAC GTCCCTCAGT 
GCTATCATTA GAACTCCCAA ATGTTATCAT ATCTCGTCGC TGAATGAAAA TGCCGCCAAA 
CGTCTGTGTC GCAGGTATTC TCAGAAACTG ACCCAGCACA CCGCCTGTCA GCTGCTGAGA 
ACTTACCCTG CTGCCACCCG CATCGACTCT TCCAACCCGA ACCCCCTCAT GTTCTGGCTC 
CATGGGATAC AGCTTGTGGC ACTCAACTAC CAGACTGATG ATCTCCCTTT ACATTTAAAT 
GCTGCAATGT TTGAGGCAAA TGGTGGTTGT GGTTATGTAT TGAAACCTCC AGTTCTGTGG 
GACAAGAACT GCCCCATGTA TCAGAAGTTT TCTCCACTAG AAAGAGATCT GGACAGCATG 
GATCCTGCAG TCTATTCTTT AACTATTGTC TCTGGTCAGA ATGTGTGCCC CAGTAATAGC 
ATGGGAAGCC CGTGCATTGA AGTCGACGTC CTGGGCATGC CTCTGGACAG CTGCCATTTC 
CGCACAAAGC CCATCCATCG AAACACCCTG AACCCCATGT GGAACGAGCA GTTTCTGTTC 
CACGTTCACT TCGAAGATCT TGTATTTCTT CGTTTTGCAG TTGTGGAAAA CAATAGTTCA 
GCGGTAACTG CTCAGAGAAT CATTCCACTG AAAGCTTTAA AACGAGGATA TCGACATCTT 
CAGCTGCGAA ACCTTCACAA TGAAGTCTTG GAGATTTCTA GTTTATTCAT TAACAGCAGA 
AGGATGGAAG AAAATTCCTC TGGCAATACC ATGTCAGCCT CTTCGATGTT TAATACAGAA 
GAAAGAAAAT GTTTGCAGAC TCACAGAGTC ACGGTGCATG GGGTCCCAGG GCCAGAGCCC 
TTTACCGTTT TCACTATTAA TGGAGGCACC AAGGCAAAGC AGCTTCTGCA GCAAATTCTG 
ACAAATGAAC AAGACATCAA ACCTGTTACC ACAGACTATT TTTTGATGGA AGAAAAATAT 
TTTATATCTA AAGAAAAGAA TGAATGTAGG AAACAACCAT TCCAGAGAGC CATTGGTCCA 
GAAGAGGAGA TCATGCAAAT TTTAAGCAGC TGGTTTCCAG AAGAGGGATA CATGGGCAGG 
ATTGTCTTAA AAACCCAGCA GGAAAACCTA GAAGAGAAAA ACATTGTTCA AGATGACAAA 
GAGGTGATCT TGAGCTCAGA GGAGGAGAGT TTCTTTGTCC AAGTGCATGA TGTTTCTCCA 
GAGCAACCTC GAACAGTCAT CAAAGCACCC CGCGTCAGCA CTGCACAGGA TGTCATTCAG 
CAGACCTTAT GCAAAGCCAA ATATTCCTAC AGCATCCTGA GCAACCCCAA TCCAAGCGAC 
TATGTGCTTT TGGAAGAGGT GGTGAAAGAC ACTACCAACA AGAAGACTAC CACACCAAAG 
TCCTCTCAGC GGGTCCTTCT GGATCAGGAG TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA 
GGTGCAGGAA AATTCATCCT TAAGCTAAAG GAGCAGGTGC AGGCATCTCG AGAAGATAAA 
AAGAAAGGCA TTTCTTTCGC AAGTGAACTC AAGAAGCTCA CCAAGTCAAC TAAACAGCCC 
CGAGGACTTA CATCACCTTC TCAGCTCTTG ACCTCAGAAA GTATCCAAAC CAAGGAGGAG 
AAACCTGTGG GTGGCTTGTC CTCCAGTGAC ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_011539849.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538151.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X2, mRNA.

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CloneID	OHu44365
Clone ID Related Accession (Same CDS sequence)	XM_006717885.3 , XM_017016310.1 , XM_017016311.2 , XM_006717885.4 , XM_017016310.2 , XM_011539849.2 , XM_011539849.3 , XM_017016311.1
Accession Version	XM_011539849.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011539849.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAAAATAAAT CATGTAATGA CAAGCTTCAG TTTGAATATA ATGAAGAAAT CCCAAAGAGG 
ATAAAGAAAG CAGATAACTC TGCTTGCAAC AAAGGAAAGG TTTATGATAT GGAACTGGGA 
GAAGAATTTT ATCTTGATCA GAATAAAAAG GAAAGCAGAC AGATTGCACC AGAGCTTTCT 
GACCTTGTAA TCTATTGTCA AGCAGTAAAA TTTCCAGGAC TGTCAACTCT AAATGCATCT 
GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCGGGC 
AGAATGAGCC CAGGGGAGAC AGCATCATTT AACAAAACAT CTGGAAAAAG TTCCTGTGAA 
GGCATTCGAC AGACCTGGGA GGAATCTTCT TCCCCTCTCA ACCCAACCAC GTCCCTCAGT 
GCTATCATTA GAACTCCCAA ATGTTATCAT ATCTCGTCGC TGAATGAAAA TGCCGCCAAA 
CGTCTGTGTC GCAGGTATTC TCAGAAACTG ACCCAGCACA CCGCCTGTCA GCTGCTGAGA 
ACTTACCCTG CTGCCACCCG CATCGACTCT TCCAACCCGA ACCCCCTCAT GTTCTGGCTC 
CATGGGATAC AGCTTGTGGC ACTCAACTAC CAGACTGATG ATCTCCCTTT ACATTTAAAT 
GCTGCAATGT TTGAGGCAAA TGGTGGTTGT GGTTATGTAT TGAAACCTCC AGTTCTGTGG 
GACAAGAACT GCCCCATGTA TCAGAAGTTT TCTCCACTAG AAAGAGATCT GGACAGCATG 
GATCCTGCAG TCTATTCTTT AACTATTGTC TCTGGTCAGA ATGTGTGCCC CAGTAATAGC 
ATGGGAAGCC CGTGCATTGA AGTCGACGTC CTGGGCATGC CTCTGGACAG CTGCCATTTC 
CGCACAAAGC CCATCCATCG AAACACCCTG AACCCCATGT GGAACGAGCA GTTTCTGTTC 
CACGTTCACT TCGAAGATCT TGTATTTCTT CGTTTTGCAG TTGTGGAAAA CAATAGTTCA 
GCGGTAACTG CTCAGAGAAT CATTCCACTG AAAGCTTTAA AACGAGGATA TCGACATCTT 
CAGCTGCGAA ACCTTCACAA TGAAGTCTTG GAGATTTCTA GTTTATTCAT TAACAGCAGA 
AGGATGGAAG AAAATTCCTC TGGCAATACC ATGTCAGCCT CTTCGATGTT TAATACAGAA 
GAAAGAAAAT GTTTGCAGAC TCACAGAGTC ACGGTGCATG GGGTCCCAGG GCCAGAGCCC 
TTTACCGTTT TCACTATTAA TGGAGGCACC AAGGCAAAGC AGCTTCTGCA GCAAATTCTG 
ACAAATGAAC AAGACATCAA ACCTGTTACC ACAGACTATT TTTTGATGGA AGAAAAATAT 
TTTATATCTA AAGAAAAGAA TGAATGTAGG AAACAACCAT TCCAGAGAGC CATTGGTCCA 
GAAGAGGAGA TCATGCAAAT TTTAAGCAGC TGGTTTCCAG AAGAGGGATA CATGGGCAGG 
ATTGTCTTAA AAACCCAGCA GGAAAACCTA GAAGAGAAAA ACATTGTTCA AGATGACAAA 
GAGGTGATCT TGAGCTCAGA GGAGGAGAGT TTCTTTGTCC AAGTGCATGA TGTTTCTCCA 
GAGCAACCTC GAACAGTCAT CAAAGCACCC CGCGTCAGCA CTGCACAGGA TGTCATTCAG 
CAGACCTTAT GCAAAGCCAA ATATTCCTAC AGCATCCTGA GCAACCCCAA TCCAAGCGAC 
TATGTGCTTT TGGAAGAGGT GGTGAAAGAC ACTACCAACA AGAAGACTAC CACACCAAAG 
TCCTCTCAGC GGGTCCTTCT GGATCAGGAG TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA 
GGTGCAGGAA AATTCATCCT TAAGCTAAAG GAGCAGGTGC AGGCATCTCG AGAAGATAAA 
AAGAAAGGCA TTTCTTTCGC AAGTGAACTC AAGAAGCTCA CCAAGTCAAC TAAACAGCCC 
CGAGGACTTA CATCACCTTC TCAGCTCTTG ACCTCAGAAA GTATCCAAAC CAAGGAGGAG 
AAACCTGTGG GTGGCTTGTC CTCCAGTGAC ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538151.1
CDS	1740..8690
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDADVLTASPAPNGQEDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_011539849.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539849.3

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAAAATAAAT CATGTAATGA CAAGCTTCAG TTTGAATATA ATGAAGAAAT CCCAAAGAGG 
ATAAAGAAAG CAGATAACTC TGCTTGCAAC AAAGGAAAGG TTTATGATAT GGAACTGGGA 
GAAGAATTTT ATCTTGATCA GAATAAAAAG GAAAGCAGAC AGATTGCACC AGAGCTTTCT 
GACCTTGTAA TCTATTGTCA AGCAGTAAAA TTTCCAGGAC TGTCAACTCT AAATGCATCT 
GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCGGGC 
AGAATGAGCC CAGGGGAGAC AGCATCATTT AACAAAACAT CTGGAAAAAG TTCCTGTGAA 
GGCATTCGAC AGACCTGGGA GGAATCTTCT TCCCCTCTCA ACCCAACCAC GTCCCTCAGT 
GCTATCATTA GAACTCCCAA ATGTTATCAT ATCTCGTCGC TGAATGAAAA TGCCGCCAAA 
CGTCTGTGTC GCAGGTATTC TCAGAAACTG ACCCAGCACA CCGCCTGTCA GCTGCTGAGA 
ACTTACCCTG CTGCCACCCG CATCGACTCT TCCAACCCGA ACCCCCTCAT GTTCTGGCTC 
CATGGGATAC AGCTTGTGGC ACTCAACTAC CAGACTGATG ATCTCCCTTT ACATTTAAAT 
GCTGCAATGT TTGAGGCAAA TGGTGGTTGT GGTTATGTAT TGAAACCTCC AGTTCTGTGG 
GACAAGAACT GCCCCATGTA TCAGAAGTTT TCTCCACTAG AAAGAGATCT GGACAGCATG 
GATCCTGCAG TCTATTCTTT AACTATTGTC TCTGGTCAGA ATGTGTGCCC CAGTAATAGC 
ATGGGAAGCC CGTGCATTGA AGTCGACGTC CTGGGCATGC CTCTGGACAG CTGCCATTTC 
CGCACAAAGC CCATCCATCG AAACACCCTG AACCCCATGT GGAACGAGCA GTTTCTGTTC 
CACGTTCACT TCGAAGATCT TGTATTTCTT CGTTTTGCAG TTGTGGAAAA CAATAGTTCA 
GCGGTAACTG CTCAGAGAAT CATTCCACTG AAAGCTTTAA AACGAGGATA TCGACATCTT 
CAGCTGCGAA ACCTTCACAA TGAAGTCTTG GAGATTTCTA GTTTATTCAT TAACAGCAGA 
AGGATGGAAG AAAATTCCTC TGGCAATACC ATGTCAGCCT CTTCGATGTT TAATACAGAA 
GAAAGAAAAT GTTTGCAGAC TCACAGAGTC ACGGTGCATG GGGTCCCAGG GCCAGAGCCC 
TTTACCGTTT TCACTATTAA TGGAGGCACC AAGGCAAAGC AGCTTCTGCA GCAAATTCTG 
ACAAATGAAC AAGACATCAA ACCTGTTACC ACAGACTATT TTTTGATGGA AGAAAAATAT 
TTTATATCTA AAGAAAAGAA TGAATGTAGG AAACAACCAT TCCAGAGAGC CATTGGTCCA 
GAAGAGGAGA TCATGCAAAT TTTAAGCAGC TGGTTTCCAG AAGAGGGATA CATGGGCAGG 
ATTGTCTTAA AAACCCAGCA GGAAAACCTA GAAGAGAAAA ACATTGTTCA AGATGACAAA 
GAGGTGATCT TGAGCTCAGA GGAGGAGAGT TTCTTTGTCC AAGTGCATGA TGTTTCTCCA 
GAGCAACCTC GAACAGTCAT CAAAGCACCC CGCGTCAGCA CTGCACAGGA TGTCATTCAG 
CAGACCTTAT GCAAAGCCAA ATATTCCTAC AGCATCCTGA GCAACCCCAA TCCAAGCGAC 
TATGTGCTTT TGGAAGAGGT GGTGAAAGAC ACTACCAACA AGAAGACTAC CACACCAAAG 
TCCTCTCAGC GGGTCCTTCT GGATCAGGAG TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA 
GGTGCAGGAA AATTCATCCT TAAGCTAAAG GAGCAGGTGC AGGCATCTCG AGAAGATAAA 
AAGAAAGGCA TTTCTTTCGC AAGTGAACTC AAGAAGCTCA CCAAGTCAAC TAAACAGCCC 
CGAGGACTTA CATCACCTTC TCAGCTCTTG ACCTCAGAAA GTATCCAAAC CAAGGAGGAG 
AAACCTGTGG GTGGCTTGTC CTCCAGTGAC ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_017016311.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871800.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X4, mRNA.

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CloneID	OHu44365
Clone ID Related Accession (Same CDS sequence)	XM_006717885.3 , XM_017016310.1 , XM_017016311.2 , XM_006717885.4 , XM_017016310.2 , XM_011539849.2 , XM_011539849.3 , XM_017016311.1
Accession Version	XM_017016311.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAAAATAAAT CATGTAATGA CAAGCTTCAG TTTGAATATA ATGAAGAAAT CCCAAAGAGG 
ATAAAGAAAG CAGATAACTC TGCTTGCAAC AAAGGAAAGG TTTATGATAT GGAACTGGGA 
GAAGAATTTT ATCTTGATCA GAATAAAAAG GAAAGCAGAC AGATTGCACC AGAGCTTTCT 
GACCTTGTAA TCTATTGTCA AGCAGTAAAA TTTCCAGGAC TGTCAACTCT AAATGCATCT 
GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCGGGC 
AGAATGAGCC CAGGGGAGAC AGCATCATTT AACAAAACAT CTGGAAAAAG TTCCTGTGAA 
GGCATTCGAC AGACCTGGGA GGAATCTTCT TCCCCTCTCA ACCCAACCAC GTCCCTCAGT 
GCTATCATTA GAACTCCCAA ATGTTATCAT ATCTCGTCGC TGAATGAAAA TGCCGCCAAA 
CGTCTGTGTC GCAGGTATTC TCAGAAACTG ACCCAGCACA CCGCCTGTCA GCTGCTGAGA 
ACTTACCCTG CTGCCACCCG CATCGACTCT TCCAACCCGA ACCCCCTCAT GTTCTGGCTC 
CATGGGATAC AGCTTGTGGC ACTCAACTAC CAGACTGATG ATCTCCCTTT ACATTTAAAT 
GCTGCAATGT TTGAGGCAAA TGGTGGTTGT GGTTATGTAT TGAAACCTCC AGTTCTGTGG 
GACAAGAACT GCCCCATGTA TCAGAAGTTT TCTCCACTAG AAAGAGATCT GGACAGCATG 
GATCCTGCAG TCTATTCTTT AACTATTGTC TCTGGTCAGA ATGTGTGCCC CAGTAATAGC 
ATGGGAAGCC CGTGCATTGA AGTCGACGTC CTGGGCATGC CTCTGGACAG CTGCCATTTC 
CGCACAAAGC CCATCCATCG AAACACCCTG AACCCCATGT GGAACGAGCA GTTTCTGTTC 
CACGTTCACT TCGAAGATCT TGTATTTCTT CGTTTTGCAG TTGTGGAAAA CAATAGTTCA 
GCGGTAACTG CTCAGAGAAT CATTCCACTG AAAGCTTTAA AACGAGGATA TCGACATCTT 
CAGCTGCGAA ACCTTCACAA TGAAGTCTTG GAGATTTCTA GTTTATTCAT TAACAGCAGA 
AGGATGGAAG AAAATTCCTC TGGCAATACC ATGTCAGCCT CTTCGATGTT TAATACAGAA 
GAAAGAAAAT GTTTGCAGAC TCACAGAGTC ACGGTGCATG GGGTCCCAGG GCCAGAGCCC 
TTTACCGTTT TCACTATTAA TGGAGGCACC AAGGCAAAGC AGCTTCTGCA GCAAATTCTG 
ACAAATGAAC AAGACATCAA ACCTGTTACC ACAGACTATT TTTTGATGGA AGAAAAATAT 
TTTATATCTA AAGAAAAGAA TGAATGTAGG AAACAACCAT TCCAGAGAGC CATTGGTCCA 
GAAGAGGAGA TCATGCAAAT TTTAAGCAGC TGGTTTCCAG AAGAGGGATA CATGGGCAGG 
ATTGTCTTAA AAACCCAGCA GGAAAACCTA GAAGAGAAAA ACATTGTTCA AGATGACAAA 
GAGGTGATCT TGAGCTCAGA GGAGGAGAGT TTCTTTGTCC AAGTGCATGA TGTTTCTCCA 
GAGCAACCTC GAACAGTCAT CAAAGCACCC CGCGTCAGCA CTGCACAGGA TGTCATTCAG 
CAGACCTTAT GCAAAGCCAA ATATTCCTAC AGCATCCTGA GCAACCCCAA TCCAAGCGAC 
TATGTGCTTT TGGAAGAGGT GGTGAAAGAC ACTACCAACA AGAAGACTAC CACACCAAAG 
TCCTCTCAGC GGGTCCTTCT GGATCAGGAG TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA 
GGTGCAGGAA AATTCATCCT TAAGCTAAAG GAGCAGGTGC AGGCATCTCG AGAAGATAAA 
AAGAAAGGCA TTTCTTTCGC AAGTGAACTC AAGAAGCTCA CCAAGTCAAC TAAACAGCCC 
CGAGGACTTA CATCACCTTC TCAGCTCTTG ACCTCAGAAA GTATCCAAAC CAAGGAGGAG 
AAACCTGTGG GTGGCTTGTC CTCCAGTGAC ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871800.1
CDS	583..7533
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDADVLTASPAPNGQEDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_017016311.1
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016311.1

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGCAGAT 
GTCCTTACTG CCTCCCCTGC TCCTAACGGT CAGGAAGACA ACATTCTGGA AGACAGACCT 
GAAAATAAAT CATGTAATGA CAAGCTTCAG TTTGAATATA ATGAAGAAAT CCCAAAGAGG 
ATAAAGAAAG CAGATAACTC TGCTTGCAAC AAAGGAAAGG TTTATGATAT GGAACTGGGA 
GAAGAATTTT ATCTTGATCA GAATAAAAAG GAAAGCAGAC AGATTGCACC AGAGCTTTCT 
GACCTTGTAA TCTATTGTCA AGCAGTAAAA TTTCCAGGAC TGTCAACTCT AAATGCATCT 
GGCTCTAGCA GAGGAAAAGA AAGGAAAAGC AGGAAGTCCA TTTTTGGCAA CAATCCGGGC 
AGAATGAGCC CAGGGGAGAC AGCATCATTT AACAAAACAT CTGGAAAAAG TTCCTGTGAA 
GGCATTCGAC AGACCTGGGA GGAATCTTCT TCCCCTCTCA ACCCAACCAC GTCCCTCAGT 
GCTATCATTA GAACTCCCAA ATGTTATCAT ATCTCGTCGC TGAATGAAAA TGCCGCCAAA 
CGTCTGTGTC GCAGGTATTC TCAGAAACTG ACCCAGCACA CCGCCTGTCA GCTGCTGAGA 
ACTTACCCTG CTGCCACCCG CATCGACTCT TCCAACCCGA ACCCCCTCAT GTTCTGGCTC 
CATGGGATAC AGCTTGTGGC ACTCAACTAC CAGACTGATG ATCTCCCTTT ACATTTAAAT 
GCTGCAATGT TTGAGGCAAA TGGTGGTTGT GGTTATGTAT TGAAACCTCC AGTTCTGTGG 
GACAAGAACT GCCCCATGTA TCAGAAGTTT TCTCCACTAG AAAGAGATCT GGACAGCATG 
GATCCTGCAG TCTATTCTTT AACTATTGTC TCTGGTCAGA ATGTGTGCCC CAGTAATAGC 
ATGGGAAGCC CGTGCATTGA AGTCGACGTC CTGGGCATGC CTCTGGACAG CTGCCATTTC 
CGCACAAAGC CCATCCATCG AAACACCCTG AACCCCATGT GGAACGAGCA GTTTCTGTTC 
CACGTTCACT TCGAAGATCT TGTATTTCTT CGTTTTGCAG TTGTGGAAAA CAATAGTTCA 
GCGGTAACTG CTCAGAGAAT CATTCCACTG AAAGCTTTAA AACGAGGATA TCGACATCTT 
CAGCTGCGAA ACCTTCACAA TGAAGTCTTG GAGATTTCTA GTTTATTCAT TAACAGCAGA 
AGGATGGAAG AAAATTCCTC TGGCAATACC ATGTCAGCCT CTTCGATGTT TAATACAGAA 
GAAAGAAAAT GTTTGCAGAC TCACAGAGTC ACGGTGCATG GGGTCCCAGG GCCAGAGCCC 
TTTACCGTTT TCACTATTAA TGGAGGCACC AAGGCAAAGC AGCTTCTGCA GCAAATTCTG 
ACAAATGAAC AAGACATCAA ACCTGTTACC ACAGACTATT TTTTGATGGA AGAAAAATAT 
TTTATATCTA AAGAAAAGAA TGAATGTAGG AAACAACCAT TCCAGAGAGC CATTGGTCCA 
GAAGAGGAGA TCATGCAAAT TTTAAGCAGC TGGTTTCCAG AAGAGGGATA CATGGGCAGG 
ATTGTCTTAA AAACCCAGCA GGAAAACCTA GAAGAGAAAA ACATTGTTCA AGATGACAAA 
GAGGTGATCT TGAGCTCAGA GGAGGAGAGT TTCTTTGTCC AAGTGCATGA TGTTTCTCCA 
GAGCAACCTC GAACAGTCAT CAAAGCACCC CGCGTCAGCA CTGCACAGGA TGTCATTCAG 
CAGACCTTAT GCAAAGCCAA ATATTCCTAC AGCATCCTGA GCAACCCCAA TCCAAGCGAC 
TATGTGCTTT TGGAAGAGGT GGTGAAAGAC ACTACCAACA AGAAGACTAC CACACCAAAG 
TCCTCTCAGC GGGTCCTTCT GGATCAGGAG TGTGTGTTTC AAGCCCAAAG CAAGTGGAAA 
GGTGCAGGAA AATTCATCCT TAAGCTAAAG GAGCAGGTGC AGGCATCTCG AGAAGATAAA 
AAGAAAGGCA TTTCTTTCGC AAGTGAACTC AAGAAGCTCA CCAAGTCAAC TAAACAGCCC 
CGAGGACTTA CATCACCTTC TCAGCTCTTG ACCTCAGAAA GTATCCAAAC CAAGGAGGAG 
AAACCTGTGG GTGGCTTGTC CTCCAGTGAC ACAATGGATT ACCGACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_006717888.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717951.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X5, mRNA.

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CloneID	OHu44366
Clone ID Related Accession (Same CDS sequence)	XM_006717888.3 , XM_006717888.4
Accession Version	XM_006717888.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717888.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGATGTC 
CTTACTGCCT CCCCTGCTCC TAACGGTCAG GAAGACAACA TTCTGGAAGA CAGACCTGAA 
AATAAATCAT GTAATGACAA GCTTCAGTTT GAATATAATG AAGAAATCCC AAAGAGGATA 
AAGAAAGCAG ATAACTCTGC TTGCAACAAA GGAAAGGTTT ATGATATGGA ACTGGGAGAA 
GAATTTTATC TTGATCAGAA TAAAAAGGAA AGCAGACAGA TTGCACCAGA GCTTTCTGAC 
CTTGTAATCT ATTGTCAAGC AGTAAAATTT CCAGGACTGT CAACTCTAAA TGCATCTGGC 
TCTAGCAGAG GAAAAGAAAG GAAAAGCAGG AAGTCCATTT TTGGCAACAA TCCGGGCAGA 
ATGAGCCCAG GGGAGACAGC ATCATTTAAC AAAACATCTG GAAAAAGTTC CTGTGAAGGC 
ATTCGACAGA CCTGGGAGGA ATCTTCTTCC CCTCTCAACC CAACCACGTC CCTCAGTGCT 
ATCATTAGAA CTCCCAAATG TTATCATATC TCGTCGCTGA ATGAAAATGC CGCCAAACGT 
CTGTGTCGCA GGTATTCTCA GAAACTGACC CAGCACACCG CCTGTCAGCT GCTGAGAACT 
TACCCTGCTG CCACCCGCAT CGACTCTTCC AACCCGAACC CCCTCATGTT CTGGCTCCAT 
GGGATACAGC TTGTGGCACT CAACTACCAG ACTGATGATC TCCCTTTACA TTTAAATGCT 
GCAATGTTTG AGGCAAATGG TGGTTGTGGT TATGTATTGA AACCTCCAGT TCTGTGGGAC 
AAGAACTGCC CCATGTATCA GAAGTTTTCT CCACTAGAAA GAGATCTGGA CAGCATGGAT 
CCTGCAGTCT ATTCTTTAAC TATTGTCTCT GGTCAGAATG TGTGCCCCAG TAATAGCATG 
GGAAGCCCGT GCATTGAAGT CGACGTCCTG GGCATGCCTC TGGACAGCTG CCATTTCCGC 
ACAAAGCCCA TCCATCGAAA CACCCTGAAC CCCATGTGGA ACGAGCAGTT TCTGTTCCAC 
GTTCACTTCG AAGATCTTGT ATTTCTTCGT TTTGCAGTTG TGGAAAACAA TAGTTCAGCG 
GTAACTGCTC AGAGAATCAT TCCACTGAAA GCTTTAAAAC GAGGATATCG ACATCTTCAG 
CTGCGAAACC TTCACAATGA AGTCTTGGAG ATTTCTAGTT TATTCATTAA CAGCAGAAGG 
ATGGAAGAAA ATTCCTCTGG CAATACCATG TCAGCCTCTT CGATGTTTAA TACAGAAGAA 
AGAAAATGTT TGCAGACTCA CAGAGTCACG GTGCATGGGG TCCCAGGGCC AGAGCCCTTT 
ACCGTTTTCA CTATTAATGG AGGCACCAAG GCAAAGCAGC TTCTGCAGCA AATTCTGACA 
AATGAACAAG ACATCAAACC TGTTACCACA GACTATTTTT TGATGGAAGA AAAATATTTT 
ATATCTAAAG AAAAGAATGA ATGTAGGAAA CAACCATTCC AGAGAGCCAT TGGTCCAGAA 
GAGGAGATCA TGCAAATTTT AAGCAGCTGG TTTCCAGAAG AGGGATACAT GGGCAGGATT 
GTCTTAAAAA CCCAGCAGGA AAACCTAGAA GAGAAAAACA TTGTTCAAGA TGACAAAGAG 
GTGATCTTGA GCTCAGAGGA GGAGAGTTTC TTTGTCCAAG TGCATGATGT TTCTCCAGAG 
CAACCTCGAA CAGTCATCAA AGCACCCCGC GTCAGCACTG CACAGGATGT CATTCAGCAG 
ACCTTATGCA AAGCCAAATA TTCCTACAGC ATCCTGAGCA ACCCCAATCC AAGCGACTAT 
GTGCTTTTGG AAGAGGTGGT GAAAGACACT ACCAACAAGA AGACTACCAC ACCAAAGTCC 
TCTCAGCGGG TCCTTCTGGA TCAGGAGTGT GTGTTTCAAG CCCAAAGCAA GTGGAAAGGT 
GCAGGAAAAT TCATCCTTAA GCTAAAGGAG CAGGTGCAGG CATCTCGAGA AGATAAAAAG 
AAAGGCATTT CTTTCGCAAG TGAACTCAAG AAGCTCACCA AGTCAACTAA ACAGCCCCGA 
GGACTTACAT CACCTTCTCA GCTCTTGACC TCAGAAAGTA TCCAAACCAA GGAGGAGAAA 
CCTGTGGGTG GCTTGTCCTC CAGTGACACA ATGGATTACC GACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717951.1
CDS	583..7530
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDDVLTASPAPNGQEDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_006717888.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717888.3

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGATGTC 
CTTACTGCCT CCCCTGCTCC TAACGGTCAG GAAGACAACA TTCTGGAAGA CAGACCTGAA 
AATAAATCAT GTAATGACAA GCTTCAGTTT GAATATAATG AAGAAATCCC AAAGAGGATA 
AAGAAAGCAG ATAACTCTGC TTGCAACAAA GGAAAGGTTT ATGATATGGA ACTGGGAGAA 
GAATTTTATC TTGATCAGAA TAAAAAGGAA AGCAGACAGA TTGCACCAGA GCTTTCTGAC 
CTTGTAATCT ATTGTCAAGC AGTAAAATTT CCAGGACTGT CAACTCTAAA TGCATCTGGC 
TCTAGCAGAG GAAAAGAAAG GAAAAGCAGG AAGTCCATTT TTGGCAACAA TCCGGGCAGA 
ATGAGCCCAG GGGAGACAGC ATCATTTAAC AAAACATCTG GAAAAAGTTC CTGTGAAGGC 
ATTCGACAGA CCTGGGAGGA ATCTTCTTCC CCTCTCAACC CAACCACGTC CCTCAGTGCT 
ATCATTAGAA CTCCCAAATG TTATCATATC TCGTCGCTGA ATGAAAATGC CGCCAAACGT 
CTGTGTCGCA GGTATTCTCA GAAACTGACC CAGCACACCG CCTGTCAGCT GCTGAGAACT 
TACCCTGCTG CCACCCGCAT CGACTCTTCC AACCCGAACC CCCTCATGTT CTGGCTCCAT 
GGGATACAGC TTGTGGCACT CAACTACCAG ACTGATGATC TCCCTTTACA TTTAAATGCT 
GCAATGTTTG AGGCAAATGG TGGTTGTGGT TATGTATTGA AACCTCCAGT TCTGTGGGAC 
AAGAACTGCC CCATGTATCA GAAGTTTTCT CCACTAGAAA GAGATCTGGA CAGCATGGAT 
CCTGCAGTCT ATTCTTTAAC TATTGTCTCT GGTCAGAATG TGTGCCCCAG TAATAGCATG 
GGAAGCCCGT GCATTGAAGT CGACGTCCTG GGCATGCCTC TGGACAGCTG CCATTTCCGC 
ACAAAGCCCA TCCATCGAAA CACCCTGAAC CCCATGTGGA ACGAGCAGTT TCTGTTCCAC 
GTTCACTTCG AAGATCTTGT ATTTCTTCGT TTTGCAGTTG TGGAAAACAA TAGTTCAGCG 
GTAACTGCTC AGAGAATCAT TCCACTGAAA GCTTTAAAAC GAGGATATCG ACATCTTCAG 
CTGCGAAACC TTCACAATGA AGTCTTGGAG ATTTCTAGTT TATTCATTAA CAGCAGAAGG 
ATGGAAGAAA ATTCCTCTGG CAATACCATG TCAGCCTCTT CGATGTTTAA TACAGAAGAA 
AGAAAATGTT TGCAGACTCA CAGAGTCACG GTGCATGGGG TCCCAGGGCC AGAGCCCTTT 
ACCGTTTTCA CTATTAATGG AGGCACCAAG GCAAAGCAGC TTCTGCAGCA AATTCTGACA 
AATGAACAAG ACATCAAACC TGTTACCACA GACTATTTTT TGATGGAAGA AAAATATTTT 
ATATCTAAAG AAAAGAATGA ATGTAGGAAA CAACCATTCC AGAGAGCCAT TGGTCCAGAA 
GAGGAGATCA TGCAAATTTT AAGCAGCTGG TTTCCAGAAG AGGGATACAT GGGCAGGATT 
GTCTTAAAAA CCCAGCAGGA AAACCTAGAA GAGAAAAACA TTGTTCAAGA TGACAAAGAG 
GTGATCTTGA GCTCAGAGGA GGAGAGTTTC TTTGTCCAAG TGCATGATGT TTCTCCAGAG 
CAACCTCGAA CAGTCATCAA AGCACCCCGC GTCAGCACTG CACAGGATGT CATTCAGCAG 
ACCTTATGCA AAGCCAAATA TTCCTACAGC ATCCTGAGCA ACCCCAATCC AAGCGACTAT 
GTGCTTTTGG AAGAGGTGGT GAAAGACACT ACCAACAAGA AGACTACCAC ACCAAAGTCC 
TCTCAGCGGG TCCTTCTGGA TCAGGAGTGT GTGTTTCAAG CCCAAAGCAA GTGGAAAGGT 
GCAGGAAAAT TCATCCTTAA GCTAAAGGAG CAGGTGCAGG CATCTCGAGA AGATAAAAAG 
AAAGGCATTT CTTTCGCAAG TGAACTCAAG AAGCTCACCA AGTCAACTAA ACAGCCCCGA 
GGACTTACAT CACCTTCTCA GCTCTTGACC TCAGAAAGTA TCCAAACCAA GGAGGAGAAA 
CCTGTGGGTG GCTTGTCCTC CAGTGACACA ATGGATTACC GACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_006717888.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717951.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X5, mRNA.

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CloneID	OHu44366
Clone ID Related Accession (Same CDS sequence)	XM_006717888.3 , XM_006717888.4
Accession Version	XM_006717888.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006717888.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGATGTC 
CTTACTGCCT CCCCTGCTCC TAACGGTCAG GAAGACAACA TTCTGGAAGA CAGACCTGAA 
AATAAATCAT GTAATGACAA GCTTCAGTTT GAATATAATG AAGAAATCCC AAAGAGGATA 
AAGAAAGCAG ATAACTCTGC TTGCAACAAA GGAAAGGTTT ATGATATGGA ACTGGGAGAA 
GAATTTTATC TTGATCAGAA TAAAAAGGAA AGCAGACAGA TTGCACCAGA GCTTTCTGAC 
CTTGTAATCT ATTGTCAAGC AGTAAAATTT CCAGGACTGT CAACTCTAAA TGCATCTGGC 
TCTAGCAGAG GAAAAGAAAG GAAAAGCAGG AAGTCCATTT TTGGCAACAA TCCGGGCAGA 
ATGAGCCCAG GGGAGACAGC ATCATTTAAC AAAACATCTG GAAAAAGTTC CTGTGAAGGC 
ATTCGACAGA CCTGGGAGGA ATCTTCTTCC CCTCTCAACC CAACCACGTC CCTCAGTGCT 
ATCATTAGAA CTCCCAAATG TTATCATATC TCGTCGCTGA ATGAAAATGC CGCCAAACGT 
CTGTGTCGCA GGTATTCTCA GAAACTGACC CAGCACACCG CCTGTCAGCT GCTGAGAACT 
TACCCTGCTG CCACCCGCAT CGACTCTTCC AACCCGAACC CCCTCATGTT CTGGCTCCAT 
GGGATACAGC TTGTGGCACT CAACTACCAG ACTGATGATC TCCCTTTACA TTTAAATGCT 
GCAATGTTTG AGGCAAATGG TGGTTGTGGT TATGTATTGA AACCTCCAGT TCTGTGGGAC 
AAGAACTGCC CCATGTATCA GAAGTTTTCT CCACTAGAAA GAGATCTGGA CAGCATGGAT 
CCTGCAGTCT ATTCTTTAAC TATTGTCTCT GGTCAGAATG TGTGCCCCAG TAATAGCATG 
GGAAGCCCGT GCATTGAAGT CGACGTCCTG GGCATGCCTC TGGACAGCTG CCATTTCCGC 
ACAAAGCCCA TCCATCGAAA CACCCTGAAC CCCATGTGGA ACGAGCAGTT TCTGTTCCAC 
GTTCACTTCG AAGATCTTGT ATTTCTTCGT TTTGCAGTTG TGGAAAACAA TAGTTCAGCG 
GTAACTGCTC AGAGAATCAT TCCACTGAAA GCTTTAAAAC GAGGATATCG ACATCTTCAG 
CTGCGAAACC TTCACAATGA AGTCTTGGAG ATTTCTAGTT TATTCATTAA CAGCAGAAGG 
ATGGAAGAAA ATTCCTCTGG CAATACCATG TCAGCCTCTT CGATGTTTAA TACAGAAGAA 
AGAAAATGTT TGCAGACTCA CAGAGTCACG GTGCATGGGG TCCCAGGGCC AGAGCCCTTT 
ACCGTTTTCA CTATTAATGG AGGCACCAAG GCAAAGCAGC TTCTGCAGCA AATTCTGACA 
AATGAACAAG ACATCAAACC TGTTACCACA GACTATTTTT TGATGGAAGA AAAATATTTT 
ATATCTAAAG AAAAGAATGA ATGTAGGAAA CAACCATTCC AGAGAGCCAT TGGTCCAGAA 
GAGGAGATCA TGCAAATTTT AAGCAGCTGG TTTCCAGAAG AGGGATACAT GGGCAGGATT 
GTCTTAAAAA CCCAGCAGGA AAACCTAGAA GAGAAAAACA TTGTTCAAGA TGACAAAGAG 
GTGATCTTGA GCTCAGAGGA GGAGAGTTTC TTTGTCCAAG TGCATGATGT TTCTCCAGAG 
CAACCTCGAA CAGTCATCAA AGCACCCCGC GTCAGCACTG CACAGGATGT CATTCAGCAG 
ACCTTATGCA AAGCCAAATA TTCCTACAGC ATCCTGAGCA ACCCCAATCC AAGCGACTAT 
GTGCTTTTGG AAGAGGTGGT GAAAGACACT ACCAACAAGA AGACTACCAC ACCAAAGTCC 
TCTCAGCGGG TCCTTCTGGA TCAGGAGTGT GTGTTTCAAG CCCAAAGCAA GTGGAAAGGT 
GCAGGAAAAT TCATCCTTAA GCTAAAGGAG CAGGTGCAGG CATCTCGAGA AGATAAAAAG 
AAAGGCATTT CTTTCGCAAG TGAACTCAAG AAGCTCACCA AGTCAACTAA ACAGCCCCGA 
GGACTTACAT CACCTTCTCA GCTCTTGACC TCAGAAAGTA TCCAAACCAA GGAGGAGAAA 
CCTGTGGGTG GCTTGTCCTC CAGTGACACA ATGGATTACC GACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717951.1
CDS	583..7530
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDDVLTASPAPNGQEDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_006717888.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717888.4

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGGCCA GTGTCCTCCC CTGTGCTGTC TTCTTCAAAC 
AAGAGCCCAT CCAGTGCTTG GAGCAGTAGT AGCTGGCACG GGCGGATCAA AGGCGGCATG 
AAGGGATTTC AGAGCTTCAT GGTTTCAGAT AGCAACATGA GTTTTGTTGA ATTTGTTGAG 
CTGTTCAAAT CATTCAGTGT CAGGAGCCGC AAGGACCTGA AGGATCTGTT TGATGTCTAT 
GCAGTGCCCT GCAACCGATC TGGCTCCGAG TCAGCCCCAC TCTACACCAA CCTGACAATT 
GATGAAAACA CCAGCGATCT TCAGCCTGAC CTAGATCTGT TGACCAGAAA TGTCTCGGAT 
TTGGGGTTGT TCATTAAGAG TAAACAGCAG CTATCGGACA ACCAGAGGCA GATATCTGAT 
GCCATTGCTG CTGCAAGCAT TGTGACAAAT GGCACTGGGA TTGAGAGCAC ATCTCTGGGC 
ATTTTTGGGG TGGGCATACT TCAGCTCAAC GATTTCCTCG TGAATTGCCA AGGAGAACAC 
TGCACTTATG ATGAAATCCT CAGCATCATC CAGAAGTTCG AGCCTAGCAT CAGTATGTGT 
CATCAGGGAC TAATGTCATT TGAAGGGTTT GCCAGGTTTC TGATGGATAA AGAAAATTTT 
GCCTCAAAAA ATGATGAGTC ACAGGAGAAC ATTAAAGAAC TGCAGCTACC CCTCTCATAC 
TATTACATCG AATCTTCGCA CAATACCTAC CTCACGGGCC ATCAGCTCAA AGGAGAATCC 
TCGGTAGAAC TCTACAGCCA GGTCCTTTTG CAAGGCTGTC GAAGTGTAGA ATTGGACTGC 
TGGGACGGAG ACGATGGGAT GCCCATCATT TATCATGGAC ATACGCTGAC AACCAAGATC 
CCCTTCAAGG AAGTGGTTGA AGCCATTGAT CGCAGTGCCT TCATCAACTC TGACCTGCCA 
ATCATCATAT CGATTGAGAA CCACTGTTCA TTGCCTCAGC AACGAAAAAT GGCAGAAATT 
TTCAAGACTG TGTTTGGAGA AAAGCTGGTG ACTAAATTCT TATTTGAGAC TGATTTCTCA 
GATGATCCAA TGCTTCCTTC ACCTGACCAA CTCAGAAAGA AAGTTCTTCT TAAAAACAAG 
AAGCTAAAAG CCCATCAGAC GCCAGTGGAT ATCTTAAAGC AAAAGGCTCA TCAGTTAGCA 
TCTATGCAAG TGCAGGCTTA TAATGGTGGG AATGCCAACC CCCGACCTGC CAATAATGAG 
GAAGAGGAAG ATGAGGAGGA CGAATATGAT TATGACTATG AATCCCTTTC TGATGATGTC 
CTTACTGCCT CCCCTGCTCC TAACGGTCAG GAAGACAACA TTCTGGAAGA CAGACCTGAA 
AATAAATCAT GTAATGACAA GCTTCAGTTT GAATATAATG AAGAAATCCC AAAGAGGATA 
AAGAAAGCAG ATAACTCTGC TTGCAACAAA GGAAAGGTTT ATGATATGGA ACTGGGAGAA 
GAATTTTATC TTGATCAGAA TAAAAAGGAA AGCAGACAGA TTGCACCAGA GCTTTCTGAC 
CTTGTAATCT ATTGTCAAGC AGTAAAATTT CCAGGACTGT CAACTCTAAA TGCATCTGGC 
TCTAGCAGAG GAAAAGAAAG GAAAAGCAGG AAGTCCATTT TTGGCAACAA TCCGGGCAGA 
ATGAGCCCAG GGGAGACAGC ATCATTTAAC AAAACATCTG GAAAAAGTTC CTGTGAAGGC 
ATTCGACAGA CCTGGGAGGA ATCTTCTTCC CCTCTCAACC CAACCACGTC CCTCAGTGCT 
ATCATTAGAA CTCCCAAATG TTATCATATC TCGTCGCTGA ATGAAAATGC CGCCAAACGT 
CTGTGTCGCA GGTATTCTCA GAAACTGACC CAGCACACCG CCTGTCAGCT GCTGAGAACT 
TACCCTGCTG CCACCCGCAT CGACTCTTCC AACCCGAACC CCCTCATGTT CTGGCTCCAT 
GGGATACAGC TTGTGGCACT CAACTACCAG ACTGATGATC TCCCTTTACA TTTAAATGCT 
GCAATGTTTG AGGCAAATGG TGGTTGTGGT TATGTATTGA AACCTCCAGT TCTGTGGGAC 
AAGAACTGCC CCATGTATCA GAAGTTTTCT CCACTAGAAA GAGATCTGGA CAGCATGGAT 
CCTGCAGTCT ATTCTTTAAC TATTGTCTCT GGTCAGAATG TGTGCCCCAG TAATAGCATG 
GGAAGCCCGT GCATTGAAGT CGACGTCCTG GGCATGCCTC TGGACAGCTG CCATTTCCGC 
ACAAAGCCCA TCCATCGAAA CACCCTGAAC CCCATGTGGA ACGAGCAGTT TCTGTTCCAC 
GTTCACTTCG AAGATCTTGT ATTTCTTCGT TTTGCAGTTG TGGAAAACAA TAGTTCAGCG 
GTAACTGCTC AGAGAATCAT TCCACTGAAA GCTTTAAAAC GAGGATATCG ACATCTTCAG 
CTGCGAAACC TTCACAATGA AGTCTTGGAG ATTTCTAGTT TATTCATTAA CAGCAGAAGG 
ATGGAAGAAA ATTCCTCTGG CAATACCATG TCAGCCTCTT CGATGTTTAA TACAGAAGAA 
AGAAAATGTT TGCAGACTCA CAGAGTCACG GTGCATGGGG TCCCAGGGCC AGAGCCCTTT 
ACCGTTTTCA CTATTAATGG AGGCACCAAG GCAAAGCAGC TTCTGCAGCA AATTCTGACA 
AATGAACAAG ACATCAAACC TGTTACCACA GACTATTTTT TGATGGAAGA AAAATATTTT 
ATATCTAAAG AAAAGAATGA ATGTAGGAAA CAACCATTCC AGAGAGCCAT TGGTCCAGAA 
GAGGAGATCA TGCAAATTTT AAGCAGCTGG TTTCCAGAAG AGGGATACAT GGGCAGGATT 
GTCTTAAAAA CCCAGCAGGA AAACCTAGAA GAGAAAAACA TTGTTCAAGA TGACAAAGAG 
GTGATCTTGA GCTCAGAGGA GGAGAGTTTC TTTGTCCAAG TGCATGATGT TTCTCCAGAG 
CAACCTCGAA CAGTCATCAA AGCACCCCGC GTCAGCACTG CACAGGATGT CATTCAGCAG 
ACCTTATGCA AAGCCAAATA TTCCTACAGC ATCCTGAGCA ACCCCAATCC AAGCGACTAT 
GTGCTTTTGG AAGAGGTGGT GAAAGACACT ACCAACAAGA AGACTACCAC ACCAAAGTCC 
TCTCAGCGGG TCCTTCTGGA TCAGGAGTGT GTGTTTCAAG CCCAAAGCAA GTGGAAAGGT 
GCAGGAAAAT TCATCCTTAA GCTAAAGGAG CAGGTGCAGG CATCTCGAGA AGATAAAAAG 
AAAGGCATTT CTTTCGCAAG TGAACTCAAG AAGCTCACCA AGTCAACTAA ACAGCCCCGA 
GGACTTACAT CACCTTCTCA GCTCTTGACC TCAGAAAGTA TCCAAACCAA GGAGGAGAAA 
CCTGTGGGTG GCTTGTCCTC CAGTGACACA ATGGATTACC GACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_006717889.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717952.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X6, mRNA.

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CloneID	OHu44367
Clone ID Related Accession (Same CDS sequence)	XM_006717889.3 , XM_006717889.4
Accession Version	XM_006717889.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717889.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGTGCT TGGAGCAGTA GTAGCTGGCA CGGGCGGATC 
AAAGGCGGCA TGAAGGGATT TCAGAGCTTC ATGGTTTCAG ATAGCAACAT GAGTTTTGTT 
GAATTTGTTG AGCTGTTCAA ATCATTCAGT GTCAGGAGCC GCAAGGACCT GAAGGATCTG 
TTTGATGTCT ATGCAGTGCC CTGCAACCGA TCTGGCTCCG AGTCAGCCCC ACTCTACACC 
AACCTGACAA TTGATGAAAA CACCAGCGAT CTTCAGCCTG ACCTAGATCT GTTGACCAGA 
AATGTCTCGG ATTTGGGGTT GTTCATTAAG AGTAAACAGC AGCTATCGGA CAACCAGAGG 
CAGATATCTG ATGCCATTGC TGCTGCAAGC ATTGTGACAA ATGGCACTGG GATTGAGAGC 
ACATCTCTGG GCATTTTTGG GGTGGGCATA CTTCAGCTCA ACGATTTCCT CGTGAATTGC 
CAAGGAGAAC ACTGCACTTA TGATGAAATC CTCAGCATCA TCCAGAAGTT CGAGCCTAGC 
ATCAGTATGT GTCATCAGGG ACTAATGTCA TTTGAAGGGT TTGCCAGGTT TCTGATGGAT 
AAAGAAAATT TTGCCTCAAA AAATGATGAG TCACAGGAGA ACATTAAAGA ACTGCAGCTA 
CCCCTCTCAT ACTATTACAT CGAATCTTCG CACAATACCT ACCTCACGGG CCATCAGCTC 
AAAGGAGAAT CCTCGGTAGA ACTCTACAGC CAGGTCCTTT TGCAAGGCTG TCGAAGTGTA 
GAATTGGACT GCTGGGACGG AGACGATGGG ATGCCCATCA TTTATCATGG ACATACGCTG 
ACAACCAAGA TCCCCTTCAA GGAAGTGGTT GAAGCCATTG ATCGCAGTGC CTTCATCAAC 
TCTGACCTGC CAATCATCAT ATCGATTGAG AACCACTGTT CATTGCCTCA GCAACGAAAA 
ATGGCAGAAA TTTTCAAGAC TGTGTTTGGA GAAAAGCTGG TGACTAAATT CTTATTTGAG 
ACTGATTTCT CAGATGATCC AATGCTTCCT TCACCTGACC AACTCAGAAA GAAAGTTCTT 
CTTAAAAACA AGAAGCTAAA AGCCCATCAG ACGCCAGTGG ATATCTTAAA GCAAAAGGCT 
CATCAGTTAG CATCTATGCA AGTGCAGGCT TATAATGGTG GGAATGCCAA CCCCCGACCT 
GCCAATAATG AGGAAGAGGA AGATGAGGAG GACGAATATG ATTATGACTA TGAATCCCTT 
TCTGATGCAG ATGTCCTTAC TGCCTCCCCT GCTCCTAACG GTCAGGAAGA CAACATTCTG 
GAAGACAGAC CTGAAAATAA ATCATGTAAT GACAAGCTTC AGTTTGAATA TAATGAAGAA 
ATCCCAAAGA GGATAAAGAA AGCAGATAAC TCTGCTTGCA ACAAAGGAAA GGTTTATGAT 
ATGGAACTGG GAGAAGAATT TTATCTTGAT CAGAATAAAA AGGAAAGCAG ACAGATTGCA 
CCAGAGCTTT CTGACCTTGT AATCTATTGT CAAGCAGTAA AATTTCCAGG ACTGTCAACT 
CTAAATGCAT CTGGCTCTAG CAGAGGAAAA GAAAGGAAAA GCAGGAAGTC CATTTTTGGC 
AACAATCCGG GCAGAATGAG CCCAGGGGAG ACAGCATCAT TTAACAAAAC ATCTGGAAAA 
AGTTCCTGTG AAGGCATTCG ACAGACCTGG GAGGAATCTT CTTCCCCTCT CAACCCAACC 
ACGTCCCTCA GTGCTATCAT TAGAACTCCC AAATGTTATC ATATCTCGTC GCTGAATGAA 
AATGCCGCCA AACGTCTGTG TCGCAGGTAT TCTCAGAAAC TGACCCAGCA CACCGCCTGT 
CAGCTGCTGA GAACTTACCC TGCTGCCACC CGCATCGACT CTTCCAACCC GAACCCCCTC 
ATGTTCTGGC TCCATGGGAT ACAGCTTGTG GCACTCAACT ACCAGACTGA TGATCTCCCT 
TTACATTTAA ATGCTGCAAT GTTTGAGGCA AATGGTGGTT GTGGTTATGT ATTGAAACCT 
CCAGTTCTGT GGGACAAGAA CTGCCCCATG TATCAGAAGT TTTCTCCACT AGAAAGAGAT 
CTGGACAGCA TGGATCCTGC AGTCTATTCT TTAACTATTG TCTCTGGTCA GAATGTGTGC 
CCCAGTAATA GCATGGGAAG CCCGTGCATT GAAGTCGACG TCCTGGGCAT GCCTCTGGAC 
AGCTGCCATT TCCGCACAAA GCCCATCCAT CGAAACACCC TGAACCCCAT GTGGAACGAG 
CAGTTTCTGT TCCACGTTCA CTTCGAAGAT CTTGTATTTC TTCGTTTTGC AGTTGTGGAA 
AACAATAGTT CAGCGGTAAC TGCTCAGAGA ATCATTCCAC TGAAAGCTTT AAAACGAGGA 
TATCGACATC TTCAGCTGCG AAACCTTCAC AATGAAGTCT TGGAGATTTC TAGTTTATTC 
ATTAACAGCA GAAGGATGGA AGAAAATTCC TCTGGCAATA CCATGTCAGC CTCTTCGATG 
TTTAATACAG AAGAAAGAAA ATGTTTGCAG ACTCACAGAG TCACGGTGCA TGGGGTCCCA 
GGGCCAGAGC CCTTTACCGT TTTCACTATT AATGGAGGCA CCAAGGCAAA GCAGCTTCTG 
CAGCAAATTC TGACAAATGA ACAAGACATC AAACCTGTTA CCACAGACTA TTTTTTGATG 
GAAGAAAAAT ATTTTATATC TAAAGAAAAG AATGAATGTA GGAAACAACC ATTCCAGAGA 
GCCATTGGTC CAGAAGAGGA GATCATGCAA ATTTTAAGCA GCTGGTTTCC AGAAGAGGGA 
TACATGGGCA GGATTGTCTT AAAAACCCAG CAGGAAAACC TAGAAGAGAA AAACATTGTT 
CAAGATGACA AAGAGGTGAT CTTGAGCTCA GAGGAGGAGA GTTTCTTTGT CCAAGTGCAT 
GATGTTTCTC CAGAGCAACC TCGAACAGTC ATCAAAGCAC CCCGCGTCAG CACTGCACAG 
GATGTCATTC AGCAGACCTT ATGCAAAGCC AAATATTCCT ACAGCATCCT GAGCAACCCC 
AATCCAAGCG ACTATGTGCT TTTGGAAGAG GTGGTGAAAG ACACTACCAA CAAGAAGACT 
ACCACACCAA AGTCCTCTCA GCGGGTCCTT CTGGATCAGG AGTGTGTGTT TCAAGCCCAA 
AGCAAGTGGA AAGGTGCAGG AAAATTCATC CTTAAGCTAA AGGAGCAGGT GCAGGCATCT 
CGAGAAGATA AAAAGAAAGG CATTTCTTTC GCAAGTGAAC TCAAGAAGCT CACCAAGTCA 
ACTAAACAGC CCCGAGGACT TACATCACCT TCTCAGCTCT TGACCTCAGA AAGTATCCAA 
ACCAAGGAGG AGAAACCTGT GGGTGGCTTG TCCTCCAGTG ACACAATGGA TTACCGACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717952.1
CDS	583..7485
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDADVLTASPAPNGQEDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_006717889.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717889.3

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGTGCT TGGAGCAGTA GTAGCTGGCA CGGGCGGATC 
AAAGGCGGCA TGAAGGGATT TCAGAGCTTC ATGGTTTCAG ATAGCAACAT GAGTTTTGTT 
GAATTTGTTG AGCTGTTCAA ATCATTCAGT GTCAGGAGCC GCAAGGACCT GAAGGATCTG 
TTTGATGTCT ATGCAGTGCC CTGCAACCGA TCTGGCTCCG AGTCAGCCCC ACTCTACACC 
AACCTGACAA TTGATGAAAA CACCAGCGAT CTTCAGCCTG ACCTAGATCT GTTGACCAGA 
AATGTCTCGG ATTTGGGGTT GTTCATTAAG AGTAAACAGC AGCTATCGGA CAACCAGAGG 
CAGATATCTG ATGCCATTGC TGCTGCAAGC ATTGTGACAA ATGGCACTGG GATTGAGAGC 
ACATCTCTGG GCATTTTTGG GGTGGGCATA CTTCAGCTCA ACGATTTCCT CGTGAATTGC 
CAAGGAGAAC ACTGCACTTA TGATGAAATC CTCAGCATCA TCCAGAAGTT CGAGCCTAGC 
ATCAGTATGT GTCATCAGGG ACTAATGTCA TTTGAAGGGT TTGCCAGGTT TCTGATGGAT 
AAAGAAAATT TTGCCTCAAA AAATGATGAG TCACAGGAGA ACATTAAAGA ACTGCAGCTA 
CCCCTCTCAT ACTATTACAT CGAATCTTCG CACAATACCT ACCTCACGGG CCATCAGCTC 
AAAGGAGAAT CCTCGGTAGA ACTCTACAGC CAGGTCCTTT TGCAAGGCTG TCGAAGTGTA 
GAATTGGACT GCTGGGACGG AGACGATGGG ATGCCCATCA TTTATCATGG ACATACGCTG 
ACAACCAAGA TCCCCTTCAA GGAAGTGGTT GAAGCCATTG ATCGCAGTGC CTTCATCAAC 
TCTGACCTGC CAATCATCAT ATCGATTGAG AACCACTGTT CATTGCCTCA GCAACGAAAA 
ATGGCAGAAA TTTTCAAGAC TGTGTTTGGA GAAAAGCTGG TGACTAAATT CTTATTTGAG 
ACTGATTTCT CAGATGATCC AATGCTTCCT TCACCTGACC AACTCAGAAA GAAAGTTCTT 
CTTAAAAACA AGAAGCTAAA AGCCCATCAG ACGCCAGTGG ATATCTTAAA GCAAAAGGCT 
CATCAGTTAG CATCTATGCA AGTGCAGGCT TATAATGGTG GGAATGCCAA CCCCCGACCT 
GCCAATAATG AGGAAGAGGA AGATGAGGAG GACGAATATG ATTATGACTA TGAATCCCTT 
TCTGATGCAG ATGTCCTTAC TGCCTCCCCT GCTCCTAACG GTCAGGAAGA CAACATTCTG 
GAAGACAGAC CTGAAAATAA ATCATGTAAT GACAAGCTTC AGTTTGAATA TAATGAAGAA 
ATCCCAAAGA GGATAAAGAA AGCAGATAAC TCTGCTTGCA ACAAAGGAAA GGTTTATGAT 
ATGGAACTGG GAGAAGAATT TTATCTTGAT CAGAATAAAA AGGAAAGCAG ACAGATTGCA 
CCAGAGCTTT CTGACCTTGT AATCTATTGT CAAGCAGTAA AATTTCCAGG ACTGTCAACT 
CTAAATGCAT CTGGCTCTAG CAGAGGAAAA GAAAGGAAAA GCAGGAAGTC CATTTTTGGC 
AACAATCCGG GCAGAATGAG CCCAGGGGAG ACAGCATCAT TTAACAAAAC ATCTGGAAAA 
AGTTCCTGTG AAGGCATTCG ACAGACCTGG GAGGAATCTT CTTCCCCTCT CAACCCAACC 
ACGTCCCTCA GTGCTATCAT TAGAACTCCC AAATGTTATC ATATCTCGTC GCTGAATGAA 
AATGCCGCCA AACGTCTGTG TCGCAGGTAT TCTCAGAAAC TGACCCAGCA CACCGCCTGT 
CAGCTGCTGA GAACTTACCC TGCTGCCACC CGCATCGACT CTTCCAACCC GAACCCCCTC 
ATGTTCTGGC TCCATGGGAT ACAGCTTGTG GCACTCAACT ACCAGACTGA TGATCTCCCT 
TTACATTTAA ATGCTGCAAT GTTTGAGGCA AATGGTGGTT GTGGTTATGT ATTGAAACCT 
CCAGTTCTGT GGGACAAGAA CTGCCCCATG TATCAGAAGT TTTCTCCACT AGAAAGAGAT 
CTGGACAGCA TGGATCCTGC AGTCTATTCT TTAACTATTG TCTCTGGTCA GAATGTGTGC 
CCCAGTAATA GCATGGGAAG CCCGTGCATT GAAGTCGACG TCCTGGGCAT GCCTCTGGAC 
AGCTGCCATT TCCGCACAAA GCCCATCCAT CGAAACACCC TGAACCCCAT GTGGAACGAG 
CAGTTTCTGT TCCACGTTCA CTTCGAAGAT CTTGTATTTC TTCGTTTTGC AGTTGTGGAA 
AACAATAGTT CAGCGGTAAC TGCTCAGAGA ATCATTCCAC TGAAAGCTTT AAAACGAGGA 
TATCGACATC TTCAGCTGCG AAACCTTCAC AATGAAGTCT TGGAGATTTC TAGTTTATTC 
ATTAACAGCA GAAGGATGGA AGAAAATTCC TCTGGCAATA CCATGTCAGC CTCTTCGATG 
TTTAATACAG AAGAAAGAAA ATGTTTGCAG ACTCACAGAG TCACGGTGCA TGGGGTCCCA 
GGGCCAGAGC CCTTTACCGT TTTCACTATT AATGGAGGCA CCAAGGCAAA GCAGCTTCTG 
CAGCAAATTC TGACAAATGA ACAAGACATC AAACCTGTTA CCACAGACTA TTTTTTGATG 
GAAGAAAAAT ATTTTATATC TAAAGAAAAG AATGAATGTA GGAAACAACC ATTCCAGAGA 
GCCATTGGTC CAGAAGAGGA GATCATGCAA ATTTTAAGCA GCTGGTTTCC AGAAGAGGGA 
TACATGGGCA GGATTGTCTT AAAAACCCAG CAGGAAAACC TAGAAGAGAA AAACATTGTT 
CAAGATGACA AAGAGGTGAT CTTGAGCTCA GAGGAGGAGA GTTTCTTTGT CCAAGTGCAT 
GATGTTTCTC CAGAGCAACC TCGAACAGTC ATCAAAGCAC CCCGCGTCAG CACTGCACAG 
GATGTCATTC AGCAGACCTT ATGCAAAGCC AAATATTCCT ACAGCATCCT GAGCAACCCC 
AATCCAAGCG ACTATGTGCT TTTGGAAGAG GTGGTGAAAG ACACTACCAA CAAGAAGACT 
ACCACACCAA AGTCCTCTCA GCGGGTCCTT CTGGATCAGG AGTGTGTGTT TCAAGCCCAA 
AGCAAGTGGA AAGGTGCAGG AAAATTCATC CTTAAGCTAA AGGAGCAGGT GCAGGCATCT 
CGAGAAGATA AAAAGAAAGG CATTTCTTTC GCAAGTGAAC TCAAGAAGCT CACCAAGTCA 
ACTAAACAGC CCCGAGGACT TACATCACCT TCTCAGCTCT TGACCTCAGA AAGTATCCAA 
ACCAAGGAGG AGAAACCTGT GGGTGGCTTG TCCTCCAGTG ACACAATGGA TTACCGACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_006717889.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717952.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X6, mRNA.

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CloneID	OHu44367
Clone ID Related Accession (Same CDS sequence)	XM_006717889.3 , XM_006717889.4
Accession Version	XM_006717889.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006717889.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGTGCT TGGAGCAGTA GTAGCTGGCA CGGGCGGATC 
AAAGGCGGCA TGAAGGGATT TCAGAGCTTC ATGGTTTCAG ATAGCAACAT GAGTTTTGTT 
GAATTTGTTG AGCTGTTCAA ATCATTCAGT GTCAGGAGCC GCAAGGACCT GAAGGATCTG 
TTTGATGTCT ATGCAGTGCC CTGCAACCGA TCTGGCTCCG AGTCAGCCCC ACTCTACACC 
AACCTGACAA TTGATGAAAA CACCAGCGAT CTTCAGCCTG ACCTAGATCT GTTGACCAGA 
AATGTCTCGG ATTTGGGGTT GTTCATTAAG AGTAAACAGC AGCTATCGGA CAACCAGAGG 
CAGATATCTG ATGCCATTGC TGCTGCAAGC ATTGTGACAA ATGGCACTGG GATTGAGAGC 
ACATCTCTGG GCATTTTTGG GGTGGGCATA CTTCAGCTCA ACGATTTCCT CGTGAATTGC 
CAAGGAGAAC ACTGCACTTA TGATGAAATC CTCAGCATCA TCCAGAAGTT CGAGCCTAGC 
ATCAGTATGT GTCATCAGGG ACTAATGTCA TTTGAAGGGT TTGCCAGGTT TCTGATGGAT 
AAAGAAAATT TTGCCTCAAA AAATGATGAG TCACAGGAGA ACATTAAAGA ACTGCAGCTA 
CCCCTCTCAT ACTATTACAT CGAATCTTCG CACAATACCT ACCTCACGGG CCATCAGCTC 
AAAGGAGAAT CCTCGGTAGA ACTCTACAGC CAGGTCCTTT TGCAAGGCTG TCGAAGTGTA 
GAATTGGACT GCTGGGACGG AGACGATGGG ATGCCCATCA TTTATCATGG ACATACGCTG 
ACAACCAAGA TCCCCTTCAA GGAAGTGGTT GAAGCCATTG ATCGCAGTGC CTTCATCAAC 
TCTGACCTGC CAATCATCAT ATCGATTGAG AACCACTGTT CATTGCCTCA GCAACGAAAA 
ATGGCAGAAA TTTTCAAGAC TGTGTTTGGA GAAAAGCTGG TGACTAAATT CTTATTTGAG 
ACTGATTTCT CAGATGATCC AATGCTTCCT TCACCTGACC AACTCAGAAA GAAAGTTCTT 
CTTAAAAACA AGAAGCTAAA AGCCCATCAG ACGCCAGTGG ATATCTTAAA GCAAAAGGCT 
CATCAGTTAG CATCTATGCA AGTGCAGGCT TATAATGGTG GGAATGCCAA CCCCCGACCT 
GCCAATAATG AGGAAGAGGA AGATGAGGAG GACGAATATG ATTATGACTA TGAATCCCTT 
TCTGATGCAG ATGTCCTTAC TGCCTCCCCT GCTCCTAACG GTCAGGAAGA CAACATTCTG 
GAAGACAGAC CTGAAAATAA ATCATGTAAT GACAAGCTTC AGTTTGAATA TAATGAAGAA 
ATCCCAAAGA GGATAAAGAA AGCAGATAAC TCTGCTTGCA ACAAAGGAAA GGTTTATGAT 
ATGGAACTGG GAGAAGAATT TTATCTTGAT CAGAATAAAA AGGAAAGCAG ACAGATTGCA 
CCAGAGCTTT CTGACCTTGT AATCTATTGT CAAGCAGTAA AATTTCCAGG ACTGTCAACT 
CTAAATGCAT CTGGCTCTAG CAGAGGAAAA GAAAGGAAAA GCAGGAAGTC CATTTTTGGC 
AACAATCCGG GCAGAATGAG CCCAGGGGAG ACAGCATCAT TTAACAAAAC ATCTGGAAAA 
AGTTCCTGTG AAGGCATTCG ACAGACCTGG GAGGAATCTT CTTCCCCTCT CAACCCAACC 
ACGTCCCTCA GTGCTATCAT TAGAACTCCC AAATGTTATC ATATCTCGTC GCTGAATGAA 
AATGCCGCCA AACGTCTGTG TCGCAGGTAT TCTCAGAAAC TGACCCAGCA CACCGCCTGT 
CAGCTGCTGA GAACTTACCC TGCTGCCACC CGCATCGACT CTTCCAACCC GAACCCCCTC 
ATGTTCTGGC TCCATGGGAT ACAGCTTGTG GCACTCAACT ACCAGACTGA TGATCTCCCT 
TTACATTTAA ATGCTGCAAT GTTTGAGGCA AATGGTGGTT GTGGTTATGT ATTGAAACCT 
CCAGTTCTGT GGGACAAGAA CTGCCCCATG TATCAGAAGT TTTCTCCACT AGAAAGAGAT 
CTGGACAGCA TGGATCCTGC AGTCTATTCT TTAACTATTG TCTCTGGTCA GAATGTGTGC 
CCCAGTAATA GCATGGGAAG CCCGTGCATT GAAGTCGACG TCCTGGGCAT GCCTCTGGAC 
AGCTGCCATT TCCGCACAAA GCCCATCCAT CGAAACACCC TGAACCCCAT GTGGAACGAG 
CAGTTTCTGT TCCACGTTCA CTTCGAAGAT CTTGTATTTC TTCGTTTTGC AGTTGTGGAA 
AACAATAGTT CAGCGGTAAC TGCTCAGAGA ATCATTCCAC TGAAAGCTTT AAAACGAGGA 
TATCGACATC TTCAGCTGCG AAACCTTCAC AATGAAGTCT TGGAGATTTC TAGTTTATTC 
ATTAACAGCA GAAGGATGGA AGAAAATTCC TCTGGCAATA CCATGTCAGC CTCTTCGATG 
TTTAATACAG AAGAAAGAAA ATGTTTGCAG ACTCACAGAG TCACGGTGCA TGGGGTCCCA 
GGGCCAGAGC CCTTTACCGT TTTCACTATT AATGGAGGCA CCAAGGCAAA GCAGCTTCTG 
CAGCAAATTC TGACAAATGA ACAAGACATC AAACCTGTTA CCACAGACTA TTTTTTGATG 
GAAGAAAAAT ATTTTATATC TAAAGAAAAG AATGAATGTA GGAAACAACC ATTCCAGAGA 
GCCATTGGTC CAGAAGAGGA GATCATGCAA ATTTTAAGCA GCTGGTTTCC AGAAGAGGGA 
TACATGGGCA GGATTGTCTT AAAAACCCAG CAGGAAAACC TAGAAGAGAA AAACATTGTT 
CAAGATGACA AAGAGGTGAT CTTGAGCTCA GAGGAGGAGA GTTTCTTTGT CCAAGTGCAT 
GATGTTTCTC CAGAGCAACC TCGAACAGTC ATCAAAGCAC CCCGCGTCAG CACTGCACAG 
GATGTCATTC AGCAGACCTT ATGCAAAGCC AAATATTCCT ACAGCATCCT GAGCAACCCC 
AATCCAAGCG ACTATGTGCT TTTGGAAGAG GTGGTGAAAG ACACTACCAA CAAGAAGACT 
ACCACACCAA AGTCCTCTCA GCGGGTCCTT CTGGATCAGG AGTGTGTGTT TCAAGCCCAA 
AGCAAGTGGA AAGGTGCAGG AAAATTCATC CTTAAGCTAA AGGAGCAGGT GCAGGCATCT 
CGAGAAGATA AAAAGAAAGG CATTTCTTTC GCAAGTGAAC TCAAGAAGCT CACCAAGTCA 
ACTAAACAGC CCCGAGGACT TACATCACCT TCTCAGCTCT TGACCTCAGA AAGTATCCAA 
ACCAAGGAGG AGAAACCTGT GGGTGGCTTG TCCTCCAGTG ACACAATGGA TTACCGACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717952.1
CDS	583..7485
Translation	MTSEEMTASVLIPVTQRKVVSAQSAADESSEKVSDINISKAHTVRRSGETSHTISQLNKLKEEPSGSNLPKILSIAREKIVSDENSNEKCWEKIMPDSAKNLNINCNNILRNHQHGLPQRQFYEMYNSVAEEDLCLETGIPSPLERKVFPGIQLELDRPSMGISPLGNQSVIIETGRAHPDSRRAVFHFHYEVDRRMSDTFCTLSENLILDDCGNCVPLPGGEEKQKKNYVAYTCKLMELAKNCDNKNEQLQCDHCDTLNDKYFCFEGSCEKVDMVYSGDSFCRKDFTDSQAAKTFLSHFEDFPDNCDDVEEDAFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIVRTLPSGHIGLTAWSYIDQKRNGPLLPCGRVMEPPSTVEIRQDGSQRLSEAQWYPIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPISAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDADVLTASPAPNGQEDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_006717889.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717889.4

ORF Insert Sequence:

ATGACTTCTG AAGAAATGAC AGCTTCTGTT CTCATACCTG TGACTCAGAG AAAAGTGGTT 
TCTGCCCAGT CGGCTGCAGA TGAAAGTAGT GAAAAGGTCT CAGACATCAA TATTTCAAAA 
GCACATACTG TCAGACGAAG TGGGGAGACT TCTCATACCA TCTCACAACT GAACAAACTT 
AAAGAAGAAC CTTCTGGAAG CAACTTGCCA AAGATTCTCT CAATAGCGAG GGAGAAAATA 
GTGAGTGATG AGAACAGTAA TGAAAAATGT TGGGAGAAAA TCATGCCAGA TTCTGCGAAA 
AACCTTAACA TTAACTGCAA CAACATATTG AGAAACCATC AGCATGGCCT TCCTCAGAGA 
CAATTTTATG AAATGTACAA CTCTGTTGCT GAGGAAGACT TGTGTTTAGA AACTGGAATT 
CCTTCTCCAC TGGAAAGAAA GGTGTTCCCT GGAATTCAAC TGGAACTAGA CAGACCTTCC 
ATGGGCATTA GTCCTTTAGG AAATCAGTCA GTGATCATAG AGACAGGCAG AGCACACCCT 
GACAGCAGAA GGGCAGTATT TCATTTTCAT TATGAAGTTG ACAGAAGAAT GTCAGACACT 
TTCTGTACCC TATCAGAAAA CTTAATTTTA GACGATTGTG GAAATTGTGT ACCACTACCT 
GGGGGTGAGG AGAAGCAAAA GAAAAACTAT GTGGCATATA CCTGTAAACT GATGGAATTG 
GCCAAAAATT GTGATAATAA GAATGAGCAG CTGCAGTGTG ATCATTGTGA CACCTTGAAT 
GATAAATACT TTTGCTTTGA AGGCTCTTGT GAGAAGGTTG ACATGGTATA TTCAGGTGAT 
AGCTTTTGTA GGAAAGACTT TACTGACAGT CAAGCTGCCA AGACCTTTTT GAGCCATTTT 
GAGGACTTCC CTGATAATTG TGATGATGTA GAAGAAGACG CTTTTAAAAG CAAAAAGGAG 
CGATCCACTT TGTTAGTCAG GAGATTCTGT AAAAATGACA GAGAAGTTAA GAAATCTGTG 
TATACTGGAA CAAGAGCAAT TGTGAGAACT CTGCCTTCTG GCCACATTGG GCTGACTGCA 
TGGAGTTACA TAGATCAGAA GAGAAATGGT CCCTTACTGC CTTGTGGGAG AGTAATGGAA 
CCCCCGTCAA CAGTGGAGAT AAGGCAAGAT GGGAGCCAAC GTCTGTCAGA AGCCCAGTGG 
TATCCTATCT ACAATGCAGT GAGAAGAGAA GAAACAGAAA ATACAGTTGG ATCTCTACTC 
CATTTCCTCA CCAAGCTCCC AGCCTCCGAG ACAGCCCATG GAAGGATAAG CGTTGGTCCA 
TGCTTAAAGC AATGTGTCCG AGACACTGTA TGTGAGTATC GCGCCACCCT CCAAAGGACT 
TCAATATCGC AGTACATCAC CGGTTCTCTC CTAGAAGCAA CCACGTCTTT GGGAGCAAGA 
AGTGGCCTTC TCAGTACTTT TGGAGGATCC ACTGGACGAA TGATGCTGAA AGAACGCCAG 
CCAGGCCCCT CTGTGGCCAA TTCCAATGCC CTCCCTTCAA GTTCAGCTGG GATCAGCAAG 
GAGCTGATCG ATCTGCAGCC TCTCATCCAG TTCCCAGAGG AAGTCGCCAG CATCCTGATG 
GAGCAAGAGC AGACTATTTA CCGCAGGGTC TTGCCAGTCG ACTACCTTTG CTTCTTAACA 
CGGGACTTGG GCACTCCTGA ATGCCAGAGC TCCTTGCCCT GCCTCAAAGC ATCCATCTCA 
GCGTCGATTC TTACCACTCA GAATGGAGAG CACAATGCCC TTGAAGATCT GGTGATGAGG 
TTTAATGAGG TGAGCTCCTG GGTGACATGG CTGATCCTCA CGGCAGGCTC CATGGAGGAG 
AAGCGAGAAG TCTTTTCATA TTTGGTGCAT GTGGCCAAAT GCTGCTGGAA CATGGGCAAC 
TACAACGCTG TCATGGAGTT CTTGGCTGGC CTCAGGTCAA GAAAAGTTTT AAAAATGTGG 
CAGTTCATGG ACCAGTCTGA TATTGAGACC ATGAGGAGCC TGAAGGATGC TATGGCCCAG 
CATGAGTCCT CTTGTGAGTA CAGAAAGGTG GTGACACGTG CCCTGCACAT CCCTGGCTGT 
AAGGTGGTTC CATTCTGTGG GGTGTTTCTG AAGGAGCTCT GTGAAGTGCT TGACGGCGCC 
TCCGGTCTCA TGAAGCTTTG CCCGCGGTAC AATTCCCAAG AAGAAACTTT AGAGTTTGTA 
GCAGATTACA GTGGACAAGA TAATTTCTTA CAACGAGTGG GACAAAATGG CTTAAAGAAT 
TCGGAGAAGG AGTCCACTGT CAACAGCATC TTTCAGGTCA TCCGGAGCTG CAATCGAAGT 
CTGGAGACAG ACGAGGAGGA CAGCCCCAGT GAAGGAAACA GCTCCAGGAA AAGCTCCTTG 
AAGGATAAAA GCCGATGGCA GTTTATAATT GGAGATTTGT TGGATTCAGA CAATGACATC 
TTTGAGCAAT CCAAAGAATA CGACTCTCAT GGTTCAGAGG ACTCACAGAA GGCCTTCGAC 
CATGGGACGG AGCTCATCCC TTGGTACGTG CTGTCCATCC AAGCCGATGT GCACCAGTTC 
CTGCTGCAGG GGGCCACGGT CATCCACTAC GACCAGGACA CACACCTCTC TGCCCGCTGC 
TTCCTCCAGC TTCAGCCCGA CAATAGCACC TTGACCTGGG TAAAGCCCAC AACTGCCTCC 
CCAGCCAGCA GTAAAGCAAA ACTTGGTGTA CTTAATAACA CAGCTGAGCC TGGAAAATTC 
CCACTACTGG GTAATGCTGG ATTAAGTAGC CTGACGGAAG GGGTCTTGGA TCTTTTTGCA 
GTGAAGGCTG TATACATGGG CCACCCTGGC ATTGATATAC ACACTGTGTG TGTTCAGAAC 
AAACTGGGTA GCATGTTCCT GTCAGAGACT GGTGTGACAT TGCTCTATGG GCTTCAGACC 
ACAGACAACA GATTATTGCA CTTCGTGGCA CCAAAGCACA CAGCTAAAAT GCTCTTCAGC 
GGATTATTGG AACTCACTAG AGCTGTGAGA AAGATGAGGA AATTCCCTGA CCAAAGACAG 
CAGTGGCTGC GGAAACAGTA CGTCAGCCTT TATCAGGAGG ATGGACGGTA TGAAGGCCCA 
ACTTTGGCTC ACGCTGTGGA GTTGTTTGGT GGCAGACGGT GGAGTGCTCG AAACCCCAGC 
CCCGGAACAT CAGCAAAGAA TGCTGAGAAG CCCAATATGC AGAGAAACAA TACCCTGGGC 
ATAAGCACTA CCAAGAAAAA GAAGAAAATC CTCATGAGGG GTGAGAGTGG AGAGGTAACT 
GACGATGAGA TGGCAACCCG AAAGGCCAAG ATGCACAAAG AGTGTCGAAG CCGGAGTGGT 
TCTGATCCTC AAGACATTAA TGAACAAGAA GAATCAGAGG TGAATGCCAT CGCTAACCCT 
CCAAACCCCC TCCCTTCCAG AAGAGCCCAC TCTTTGACCA CAGCTGGGTC CCCCAACTTG 
GCTGCCGGGA CGTCATCTCC CATCAGTGCT TGGAGCAGTA GTAGCTGGCA CGGGCGGATC 
AAAGGCGGCA TGAAGGGATT TCAGAGCTTC ATGGTTTCAG ATAGCAACAT GAGTTTTGTT 
GAATTTGTTG AGCTGTTCAA ATCATTCAGT GTCAGGAGCC GCAAGGACCT GAAGGATCTG 
TTTGATGTCT ATGCAGTGCC CTGCAACCGA TCTGGCTCCG AGTCAGCCCC ACTCTACACC 
AACCTGACAA TTGATGAAAA CACCAGCGAT CTTCAGCCTG ACCTAGATCT GTTGACCAGA 
AATGTCTCGG ATTTGGGGTT GTTCATTAAG AGTAAACAGC AGCTATCGGA CAACCAGAGG 
CAGATATCTG ATGCCATTGC TGCTGCAAGC ATTGTGACAA ATGGCACTGG GATTGAGAGC 
ACATCTCTGG GCATTTTTGG GGTGGGCATA CTTCAGCTCA ACGATTTCCT CGTGAATTGC 
CAAGGAGAAC ACTGCACTTA TGATGAAATC CTCAGCATCA TCCAGAAGTT CGAGCCTAGC 
ATCAGTATGT GTCATCAGGG ACTAATGTCA TTTGAAGGGT TTGCCAGGTT TCTGATGGAT 
AAAGAAAATT TTGCCTCAAA AAATGATGAG TCACAGGAGA ACATTAAAGA ACTGCAGCTA 
CCCCTCTCAT ACTATTACAT CGAATCTTCG CACAATACCT ACCTCACGGG CCATCAGCTC 
AAAGGAGAAT CCTCGGTAGA ACTCTACAGC CAGGTCCTTT TGCAAGGCTG TCGAAGTGTA 
GAATTGGACT GCTGGGACGG AGACGATGGG ATGCCCATCA TTTATCATGG ACATACGCTG 
ACAACCAAGA TCCCCTTCAA GGAAGTGGTT GAAGCCATTG ATCGCAGTGC CTTCATCAAC 
TCTGACCTGC CAATCATCAT ATCGATTGAG AACCACTGTT CATTGCCTCA GCAACGAAAA 
ATGGCAGAAA TTTTCAAGAC TGTGTTTGGA GAAAAGCTGG TGACTAAATT CTTATTTGAG 
ACTGATTTCT CAGATGATCC AATGCTTCCT TCACCTGACC AACTCAGAAA GAAAGTTCTT 
CTTAAAAACA AGAAGCTAAA AGCCCATCAG ACGCCAGTGG ATATCTTAAA GCAAAAGGCT 
CATCAGTTAG CATCTATGCA AGTGCAGGCT TATAATGGTG GGAATGCCAA CCCCCGACCT 
GCCAATAATG AGGAAGAGGA AGATGAGGAG GACGAATATG ATTATGACTA TGAATCCCTT 
TCTGATGCAG ATGTCCTTAC TGCCTCCCCT GCTCCTAACG GTCAGGAAGA CAACATTCTG 
GAAGACAGAC CTGAAAATAA ATCATGTAAT GACAAGCTTC AGTTTGAATA TAATGAAGAA 
ATCCCAAAGA GGATAAAGAA AGCAGATAAC TCTGCTTGCA ACAAAGGAAA GGTTTATGAT 
ATGGAACTGG GAGAAGAATT TTATCTTGAT CAGAATAAAA AGGAAAGCAG ACAGATTGCA 
CCAGAGCTTT CTGACCTTGT AATCTATTGT CAAGCAGTAA AATTTCCAGG ACTGTCAACT 
CTAAATGCAT CTGGCTCTAG CAGAGGAAAA GAAAGGAAAA GCAGGAAGTC CATTTTTGGC 
AACAATCCGG GCAGAATGAG CCCAGGGGAG ACAGCATCAT TTAACAAAAC ATCTGGAAAA 
AGTTCCTGTG AAGGCATTCG ACAGACCTGG GAGGAATCTT CTTCCCCTCT CAACCCAACC 
ACGTCCCTCA GTGCTATCAT TAGAACTCCC AAATGTTATC ATATCTCGTC GCTGAATGAA 
AATGCCGCCA AACGTCTGTG TCGCAGGTAT TCTCAGAAAC TGACCCAGCA CACCGCCTGT 
CAGCTGCTGA GAACTTACCC TGCTGCCACC CGCATCGACT CTTCCAACCC GAACCCCCTC 
ATGTTCTGGC TCCATGGGAT ACAGCTTGTG GCACTCAACT ACCAGACTGA TGATCTCCCT 
TTACATTTAA ATGCTGCAAT GTTTGAGGCA AATGGTGGTT GTGGTTATGT ATTGAAACCT 
CCAGTTCTGT GGGACAAGAA CTGCCCCATG TATCAGAAGT TTTCTCCACT AGAAAGAGAT 
CTGGACAGCA TGGATCCTGC AGTCTATTCT TTAACTATTG TCTCTGGTCA GAATGTGTGC 
CCCAGTAATA GCATGGGAAG CCCGTGCATT GAAGTCGACG TCCTGGGCAT GCCTCTGGAC 
AGCTGCCATT TCCGCACAAA GCCCATCCAT CGAAACACCC TGAACCCCAT GTGGAACGAG 
CAGTTTCTGT TCCACGTTCA CTTCGAAGAT CTTGTATTTC TTCGTTTTGC AGTTGTGGAA 
AACAATAGTT CAGCGGTAAC TGCTCAGAGA ATCATTCCAC TGAAAGCTTT AAAACGAGGA 
TATCGACATC TTCAGCTGCG AAACCTTCAC AATGAAGTCT TGGAGATTTC TAGTTTATTC 
ATTAACAGCA GAAGGATGGA AGAAAATTCC TCTGGCAATA CCATGTCAGC CTCTTCGATG 
TTTAATACAG AAGAAAGAAA ATGTTTGCAG ACTCACAGAG TCACGGTGCA TGGGGTCCCA 
GGGCCAGAGC CCTTTACCGT TTTCACTATT AATGGAGGCA CCAAGGCAAA GCAGCTTCTG 
CAGCAAATTC TGACAAATGA ACAAGACATC AAACCTGTTA CCACAGACTA TTTTTTGATG 
GAAGAAAAAT ATTTTATATC TAAAGAAAAG AATGAATGTA GGAAACAACC ATTCCAGAGA 
GCCATTGGTC CAGAAGAGGA GATCATGCAA ATTTTAAGCA GCTGGTTTCC AGAAGAGGGA 
TACATGGGCA GGATTGTCTT AAAAACCCAG CAGGAAAACC TAGAAGAGAA AAACATTGTT 
CAAGATGACA AAGAGGTGAT CTTGAGCTCA GAGGAGGAGA GTTTCTTTGT CCAAGTGCAT 
GATGTTTCTC CAGAGCAACC TCGAACAGTC ATCAAAGCAC CCCGCGTCAG CACTGCACAG 
GATGTCATTC AGCAGACCTT ATGCAAAGCC AAATATTCCT ACAGCATCCT GAGCAACCCC 
AATCCAAGCG ACTATGTGCT TTTGGAAGAG GTGGTGAAAG ACACTACCAA CAAGAAGACT 
ACCACACCAA AGTCCTCTCA GCGGGTCCTT CTGGATCAGG AGTGTGTGTT TCAAGCCCAA 
AGCAAGTGGA AAGGTGCAGG AAAATTCATC CTTAAGCTAA AGGAGCAGGT GCAGGCATCT 
CGAGAAGATA AAAAGAAAGG CATTTCTTTC GCAAGTGAAC TCAAGAAGCT CACCAAGTCA 
ACTAAACAGC CCCGAGGACT TACATCACCT TCTCAGCTCT TGACCTCAGA AAGTATCCAA 
ACCAAGGAGG AGAAACCTGT GGGTGGCTTG TCCTCCAGTG ACACAATGGA TTACCGACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_006717890.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717953.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X7, mRNA.

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CloneID	OHu44368
Clone ID Related Accession (Same CDS sequence)	XM_006717890.2 , XM_006717890.3
Accession Version	XM_006717890.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717890.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCAG AAGGAAGTGC AGCAGGAAGA GACTTTGCAG GAATGGAAGA GGTGAGGCAG 
CTCCACGTGA GATTCTGCAA AGGGATTAAG ATTTGGCACC AGGCTTGGTT TCTGTGCAGC 
CTCTTAGGCA GAGAGCCCCA GGAGAGGGAA GCAGGCTGTC AGCTGTGGCT TTGCACGCTC 
TCAGCTGTCT TGAAGGTTGG TTGGCTCTTC CCGCTCTCTG AGGTACCCAA TTTTACCTTG 
TTAAAGGATG GATGTGGATG CTGGAGGCTT AAAGAAGACC AGATCTACAA TGCAGTGAGA 
AGAGAAGAAA CAGAAAATAC AGTTGGATCT CTACTCCATT TCCTCACCAA GCTCCCAGCC 
TCCGAGACAG CCCATGGAAG GATAAGCGTT GGTCCATGCT TAAAGCAATG TGTCCGAGAC 
ACTGTATGTG AGTATCGCGC CACCCTCCAA AGGACTTCAA TATCGCAGTA CATCACCGGT 
TCTCTCCTAG AAGCAACCAC GTCTTTGGGA GCAAGAAGTG GCCTTCTCAG TACTTTTGGA 
GGATCCACTG GACGAATGAT GCTGAAAGAA CGCCAGCCAG GCCCCTCTGT GGCCAATTCC 
AATGCCCTCC CTTCAAGTTC AGCTGGGATC AGCAAGGAGC TGATCGATCT GCAGCCTCTC 
ATCCAGTTCC CAGAGGAAGT CGCCAGCATC CTGATGGAGC AAGAGCAGAC TATTTACCGC 
AGGGTCTTGC CAGTCGACTA CCTTTGCTTC TTAACACGGG ACTTGGGCAC TCCTGAATGC 
CAGAGCTCCT TGCCCTGCCT CAAAGCATCC ATCTCAGCGT CGATTCTTAC CACTCAGAAT 
GGAGAGCACA ATGCCCTTGA AGATCTGGTG ATGAGGTTTA ATGAGGTGAG CTCCTGGGTG 
ACATGGCTGA TCCTCACGGC AGGCTCCATG GAGGAGAAGC GAGAAGTCTT TTCATATTTG 
GTGCATGTGG CCAAATGCTG CTGGAACATG GGCAACTACA ACGCTGTCAT GGAGTTCTTG 
GCTGGCCTCA GGTCAAGAAA AGTTTTAAAA ATGTGGCAGT TCATGGACCA GTCTGATATT 
GAGACCATGA GGAGCCTGAA GGATGCTATG GCCCAGCATG AGTCCTCTTG TGAGTACAGA 
AAGGTGGTGA CACGTGCCCT GCACATCCCT GGCTGTAAGG TGGTTCCATT CTGTGGGGTG 
TTTCTGAAGG AGCTCTGTGA AGTGCTTGAC GGCGCCTCCG GTCTCATGAA GCTTTGCCCG 
CGGTACAATT CCCAAGAAGA AACTTTAGAG TTTGTAGCAG ATTACAGTGG ACAAGATAAT 
TTCTTACAAC GAGTGGGACA AAATGGCTTA AAGAATTCGG AGAAGGAGTC CACTGTCAAC 
AGCATCTTTC AGGTCATCCG GAGCTGCAAT CGAAGTCTGG AGACAGACGA GGAGGACAGC 
CCCAGTGAAG GAAACAGCTC CAGGAAAAGC TCCTTGAAGG ATAAAAGCCG ATGGCAGTTT 
ATAATTGGAG ATTTGTTGGA TTCAGACAAT GACATCTTTG AGCAATCCAA AGAATACGAC 
TCTCATGGTT CAGAGGACTC ACAGAAGGCC TTCGACCATG GGACGGAGCT CATCCCTTGG 
TACGTGCTGT CCATCCAAGC CGATGTGCAC CAGTTCCTGC TGCAGGGGGC CACGGTCATC 
CACTACGACC AGGACACACA CCTCTCTGCC CGCTGCTTCC TCCAGCTTCA GCCCGACAAT 
AGCACCTTGA CCTGGGTAAA GCCCACAACT GCCTCCCCAG CCAGCAGTAA AGCAAAACTT 
GGTGTACTTA ATAACACAGC TGAGCCTGGA AAATTCCCAC TACTGGGTAA TGCTGGATTA 
AGTAGCCTGA CGGAAGGGGT CTTGGATCTT TTTGCAGTGA AGGCTGTATA CATGGGCCAC 
CCTGGCATTG ATATACACAC TGTGTGTGTT CAGAACAAAC TGGGTAGCAT GTTCCTGTCA 
GAGACTGGTG TGACATTGCT CTATGGGCTT CAGACCACAG ACAACAGATT ATTGCACTTC 
GTGGCACCAA AGCACACAGC TAAAATGCTC TTCAGCGGAT TATTGGAACT CACTAGAGCT 
GTGAGAAAGA TGAGGAAATT CCCTGACCAA AGACAGCAGT GGCTGCGGAA ACAGTACGTC 
AGCCTTTATC AGGAGGATGG ACGGTATGAA GGCCCAACTT TGGCTCACGC TGTGGAGTTG 
TTTGGTGGCA GACGGTGGAG TGCTCGAAAC CCCAGCCCCG GAACATCAGC AAAGAATGCT 
GAGAAGCCCA ATATGCAGAG AAACAATACC CTGGGCATAA GCACTACCAA GAAAAAGAAG 
AAAATCCTCA TGAGGGGTGA GAGTGGAGAG GTAACTGACG ATGAGATGGC AACCCGAAAG 
GCCAAGATGC ACAAAGAGTG TCGAAGCCGG AGTGGTTCTG ATCCTCAAGA CATTAATGAA 
CAAGAAGAAT CAGAGGTGAA TGCCATCGCT AACCCTCCAA ACCCCCTCCC TTCCAGAAGA 
GCCCACTCTT TGACCACAGC TGGGTCCCCC AACTTGGCTG CCGGGACGTC ATCTCCCATC 
AGGCCAGTGT CCTCCCCTGT GCTGTCTTCT TCAAACAAGA GCCCATCCAG TGCTTGGAGC 
AGTAGTAGCT GGCACGGGCG GATCAAAGGC GGCATGAAGG GATTTCAGAG CTTCATGGTT 
TCAGATAGCA ACATGAGTTT TGTTGAATTT GTTGAGCTGT TCAAATCATT CAGTGTCAGG 
AGCCGCAAGG ACCTGAAGGA TCTGTTTGAT GTCTATGCAG TGCCCTGCAA CCGATCTGGC 
TCCGAGTCAG CCCCACTCTA CACCAACCTG ACAATTGATG AAAACACCAG CGATCTTCAG 
CCTGACCTAG ATCTGTTGAC CAGAAATGTC TCGGATTTGG GGTTGTTCAT TAAGAGTAAA 
CAGCAGCTAT CGGACAACCA GAGGCAGATA TCTGATGCCA TTGCTGCTGC AAGCATTGTG 
ACAAATGGCA CTGGGATTGA GAGCACATCT CTGGGCATTT TTGGGGTGGG CATACTTCAG 
CTCAACGATT TCCTCGTGAA TTGCCAAGGA GAACACTGCA CTTATGATGA AATCCTCAGC 
ATCATCCAGA AGTTCGAGCC TAGCATCAGT ATGTGTCATC AGGGACTAAT GTCATTTGAA 
GGGTTTGCCA GGTTTCTGAT GGATAAAGAA AATTTTGCCT CAAAAAATGA TGAGTCACAG 
GAGAACATTA AAGAACTGCA GCTACCCCTC TCATACTATT ACATCGAATC TTCGCACAAT 
ACCTACCTCA CGGGCCATCA GCTCAAAGGA GAATCCTCGG TAGAACTCTA CAGCCAGGTC 
CTTTTGCAAG GCTGTCGAAG TGTAGAATTG GACTGCTGGG ACGGAGACGA TGGGATGCCC 
ATCATTTATC ATGGACATAC GCTGACAACC AAGATCCCCT TCAAGGAAGT GGTTGAAGCC 
ATTGATCGCA GTGCCTTCAT CAACTCTGAC CTGCCAATCA TCATATCGAT TGAGAACCAC 
TGTTCATTGC CTCAGCAACG AAAAATGGCA GAAATTTTCA AGACTGTGTT TGGAGAAAAG 
CTGGTGACTA AATTCTTATT TGAGACTGAT TTCTCAGATG ATCCAATGCT TCCTTCACCT 
GACCAACTCA GAAAGAAAGT TCTTCTTAAA AACAAGAAGC TAAAAGCCCA TCAGACGCCA 
GTGGATATCT TAAAGCAAAA GGCTCATCAG TTAGCATCTA TGCAAGTGCA GGCTTATAAT 
GGTGGGAATG CCAACCCCCG ACCTGCCAAT AATGAGGAAG AGGAAGATGA GGAGGACGAA 
TATGATTATG ACTATGAATC CCTTTCTGAT GCAGATGTCC TTACTGCCTC CCCTGCTCCT 
AACGGTCAGG AAGACAACAT TCTGGAAGAC AGACCTGAAA ATAAATCATG TAATGACAAG 
CTTCAGTTTG AATATAATGA AGAAATCCCA AAGAGGATAA AGAAAGCAGA TAACTCTGCT 
TGCAACAAAG GAAAGGTTTA TGATATGGAA CTGGGAGAAG AATTTTATCT TGATCAGAAT 
AAAAAGGAAA GCAGACAGAT TGCACCAGAG CTTTCTGACC TTGTAATCTA TTGTCAAGCA 
GTAAAATTTC CAGGACTGTC AACTCTAAAT GCATCTGGCT CTAGCAGAGG AAAAGAAAGG 
AAAAGCAGGA AGTCCATTTT TGGCAACAAT CCGGGCAGAA TGAGCCCAGG GGAGACAGCA 
TCATTTAACA AAACATCTGG AAAAAGTTCC TGTGAAGGCA TTCGACAGAC CTGGGAGGAA 
TCTTCTTCCC CTCTCAACCC AACCACGTCC CTCAGTGCTA TCATTAGAAC TCCCAAATGT 
TATCATATCT CGTCGCTGAA TGAAAATGCC GCCAAACGTC TGTGTCGCAG GTATTCTCAG 
AAACTGACCC AGCACACCGC CTGTCAGCTG CTGAGAACTT ACCCTGCTGC CACCCGCATC 
GACTCTTCCA ACCCGAACCC CCTCATGTTC TGGCTCCATG GGATACAGCT TGTGGCACTC 
AACTACCAGA CTGATGATCT CCCTTTACAT TTAAATGCTG CAATGTTTGA GGCAAATGGT 
GGTTGTGGTT ATGTATTGAA ACCTCCAGTT CTGTGGGACA AGAACTGCCC CATGTATCAG 
AAGTTTTCTC CACTAGAAAG AGATCTGGAC AGCATGGATC CTGCAGTCTA TTCTTTAACT 
ATTGTCTCTG GTCAGAATGT GTGCCCCAGT AATAGCATGG GAAGCCCGTG CATTGAAGTC 
GACGTCCTGG GCATGCCTCT GGACAGCTGC CATTTCCGCA CAAAGCCCAT CCATCGAAAC 
ACCCTGAACC CCATGTGGAA CGAGCAGTTT CTGTTCCACG TTCACTTCGA AGATCTTGTA 
TTTCTTCGTT TTGCAGTTGT GGAAAACAAT AGTTCAGCGG TAACTGCTCA GAGAATCATT 
CCACTGAAAG CTTTAAAACG AGGATATCGA CATCTTCAGC TGCGAAACCT TCACAATGAA 
GTCTTGGAGA TTTCTAGTTT ATTCATTAAC AGCAGAAGGA TGGAAGAAAA TTCCTCTGGC 
AATACCATGT CAGCCTCTTC GATGTTTAAT ACAGAAGAAA GAAAATGTTT GCAGACTCAC 
AGAGTCACGG TGCATGGGGT CCCAGGGCCA GAGCCCTTTA CCGTTTTCAC TATTAATGGA 
GGCACCAAGG CAAAGCAGCT TCTGCAGCAA ATTCTGACAA ATGAACAAGA CATCAAACCT 
GTTACCACAG ACTATTTTTT GATGGAAGAA AAATATTTTA TATCTAAAGA AAAGAATGAA 
TGTAGGAAAC AACCATTCCA GAGAGCCATT GGTCCAGAAG AGGAGATCAT GCAAATTTTA 
AGCAGCTGGT TTCCAGAAGA GGGATACATG GGCAGGATTG TCTTAAAAAC CCAGCAGGAA 
AACCTAGAAG AGAAAAACAT TGTTCAAGAT GACAAAGAGG TGATCTTGAG CTCAGAGGAG 
GAGAGTTTCT TTGTCCAAGT GCATGATGTT TCTCCAGAGC AACCTCGAAC AGTCATCAAA 
GCACCCCGCG TCAGCACTGC ACAGGATGTC ATTCAGCAGA CCTTATGCAA AGCCAAATAT 
TCCTACAGCA TCCTGAGCAA CCCCAATCCA AGCGACTATG TGCTTTTGGA AGAGGTGGTG 
AAAGACACTA CCAACAAGAA GACTACCACA CCAAAGTCCT CTCAGCGGGT CCTTCTGGAT 
CAGGAGTGTG TGTTTCAAGC CCAAAGCAAG TGGAAAGGTG CAGGAAAATT CATCCTTAAG 
CTAAAGGAGC AGGTGCAGGC ATCTCGAGAA GATAAAAAGA AAGGCATTTC TTTCGCAAGT 
GAACTCAAGA AGCTCACCAA GTCAACTAAA CAGCCCCGAG GACTTACATC ACCTTCTCAG 
CTCTTGACCT CAGAAAGTAT CCAAACCAAG GAGGAGAAAC CTGTGGGTGG CTTGTCCTCC 
AGTGACACAA TGGATTACCG ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717953.1
CDS	23..6049
Translation	MVSEGSAAGRDFAGMEEVRQLHVRFCKGIKIWHQAWFLCSLLGREPQEREAGCQLWLCTLSAVLKVGWLFPLSEVPNFTLLKDGCGCWRLKEDQIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDADVLTASPAPNGQEDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_006717890.2
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717890.2

ORF Insert Sequence:

ATGGTTTCAG AAGGAAGTGC AGCAGGAAGA GACTTTGCAG GAATGGAAGA GGTGAGGCAG 
CTCCACGTGA GATTCTGCAA AGGGATTAAG ATTTGGCACC AGGCTTGGTT TCTGTGCAGC 
CTCTTAGGCA GAGAGCCCCA GGAGAGGGAA GCAGGCTGTC AGCTGTGGCT TTGCACGCTC 
TCAGCTGTCT TGAAGGTTGG TTGGCTCTTC CCGCTCTCTG AGGTACCCAA TTTTACCTTG 
TTAAAGGATG GATGTGGATG CTGGAGGCTT AAAGAAGACC AGATCTACAA TGCAGTGAGA 
AGAGAAGAAA CAGAAAATAC AGTTGGATCT CTACTCCATT TCCTCACCAA GCTCCCAGCC 
TCCGAGACAG CCCATGGAAG GATAAGCGTT GGTCCATGCT TAAAGCAATG TGTCCGAGAC 
ACTGTATGTG AGTATCGCGC CACCCTCCAA AGGACTTCAA TATCGCAGTA CATCACCGGT 
TCTCTCCTAG AAGCAACCAC GTCTTTGGGA GCAAGAAGTG GCCTTCTCAG TACTTTTGGA 
GGATCCACTG GACGAATGAT GCTGAAAGAA CGCCAGCCAG GCCCCTCTGT GGCCAATTCC 
AATGCCCTCC CTTCAAGTTC AGCTGGGATC AGCAAGGAGC TGATCGATCT GCAGCCTCTC 
ATCCAGTTCC CAGAGGAAGT CGCCAGCATC CTGATGGAGC AAGAGCAGAC TATTTACCGC 
AGGGTCTTGC CAGTCGACTA CCTTTGCTTC TTAACACGGG ACTTGGGCAC TCCTGAATGC 
CAGAGCTCCT TGCCCTGCCT CAAAGCATCC ATCTCAGCGT CGATTCTTAC CACTCAGAAT 
GGAGAGCACA ATGCCCTTGA AGATCTGGTG ATGAGGTTTA ATGAGGTGAG CTCCTGGGTG 
ACATGGCTGA TCCTCACGGC AGGCTCCATG GAGGAGAAGC GAGAAGTCTT TTCATATTTG 
GTGCATGTGG CCAAATGCTG CTGGAACATG GGCAACTACA ACGCTGTCAT GGAGTTCTTG 
GCTGGCCTCA GGTCAAGAAA AGTTTTAAAA ATGTGGCAGT TCATGGACCA GTCTGATATT 
GAGACCATGA GGAGCCTGAA GGATGCTATG GCCCAGCATG AGTCCTCTTG TGAGTACAGA 
AAGGTGGTGA CACGTGCCCT GCACATCCCT GGCTGTAAGG TGGTTCCATT CTGTGGGGTG 
TTTCTGAAGG AGCTCTGTGA AGTGCTTGAC GGCGCCTCCG GTCTCATGAA GCTTTGCCCG 
CGGTACAATT CCCAAGAAGA AACTTTAGAG TTTGTAGCAG ATTACAGTGG ACAAGATAAT 
TTCTTACAAC GAGTGGGACA AAATGGCTTA AAGAATTCGG AGAAGGAGTC CACTGTCAAC 
AGCATCTTTC AGGTCATCCG GAGCTGCAAT CGAAGTCTGG AGACAGACGA GGAGGACAGC 
CCCAGTGAAG GAAACAGCTC CAGGAAAAGC TCCTTGAAGG ATAAAAGCCG ATGGCAGTTT 
ATAATTGGAG ATTTGTTGGA TTCAGACAAT GACATCTTTG AGCAATCCAA AGAATACGAC 
TCTCATGGTT CAGAGGACTC ACAGAAGGCC TTCGACCATG GGACGGAGCT CATCCCTTGG 
TACGTGCTGT CCATCCAAGC CGATGTGCAC CAGTTCCTGC TGCAGGGGGC CACGGTCATC 
CACTACGACC AGGACACACA CCTCTCTGCC CGCTGCTTCC TCCAGCTTCA GCCCGACAAT 
AGCACCTTGA CCTGGGTAAA GCCCACAACT GCCTCCCCAG CCAGCAGTAA AGCAAAACTT 
GGTGTACTTA ATAACACAGC TGAGCCTGGA AAATTCCCAC TACTGGGTAA TGCTGGATTA 
AGTAGCCTGA CGGAAGGGGT CTTGGATCTT TTTGCAGTGA AGGCTGTATA CATGGGCCAC 
CCTGGCATTG ATATACACAC TGTGTGTGTT CAGAACAAAC TGGGTAGCAT GTTCCTGTCA 
GAGACTGGTG TGACATTGCT CTATGGGCTT CAGACCACAG ACAACAGATT ATTGCACTTC 
GTGGCACCAA AGCACACAGC TAAAATGCTC TTCAGCGGAT TATTGGAACT CACTAGAGCT 
GTGAGAAAGA TGAGGAAATT CCCTGACCAA AGACAGCAGT GGCTGCGGAA ACAGTACGTC 
AGCCTTTATC AGGAGGATGG ACGGTATGAA GGCCCAACTT TGGCTCACGC TGTGGAGTTG 
TTTGGTGGCA GACGGTGGAG TGCTCGAAAC CCCAGCCCCG GAACATCAGC AAAGAATGCT 
GAGAAGCCCA ATATGCAGAG AAACAATACC CTGGGCATAA GCACTACCAA GAAAAAGAAG 
AAAATCCTCA TGAGGGGTGA GAGTGGAGAG GTAACTGACG ATGAGATGGC AACCCGAAAG 
GCCAAGATGC ACAAAGAGTG TCGAAGCCGG AGTGGTTCTG ATCCTCAAGA CATTAATGAA 
CAAGAAGAAT CAGAGGTGAA TGCCATCGCT AACCCTCCAA ACCCCCTCCC TTCCAGAAGA 
GCCCACTCTT TGACCACAGC TGGGTCCCCC AACTTGGCTG CCGGGACGTC ATCTCCCATC 
AGGCCAGTGT CCTCCCCTGT GCTGTCTTCT TCAAACAAGA GCCCATCCAG TGCTTGGAGC 
AGTAGTAGCT GGCACGGGCG GATCAAAGGC GGCATGAAGG GATTTCAGAG CTTCATGGTT 
TCAGATAGCA ACATGAGTTT TGTTGAATTT GTTGAGCTGT TCAAATCATT CAGTGTCAGG 
AGCCGCAAGG ACCTGAAGGA TCTGTTTGAT GTCTATGCAG TGCCCTGCAA CCGATCTGGC 
TCCGAGTCAG CCCCACTCTA CACCAACCTG ACAATTGATG AAAACACCAG CGATCTTCAG 
CCTGACCTAG ATCTGTTGAC CAGAAATGTC TCGGATTTGG GGTTGTTCAT TAAGAGTAAA 
CAGCAGCTAT CGGACAACCA GAGGCAGATA TCTGATGCCA TTGCTGCTGC AAGCATTGTG 
ACAAATGGCA CTGGGATTGA GAGCACATCT CTGGGCATTT TTGGGGTGGG CATACTTCAG 
CTCAACGATT TCCTCGTGAA TTGCCAAGGA GAACACTGCA CTTATGATGA AATCCTCAGC 
ATCATCCAGA AGTTCGAGCC TAGCATCAGT ATGTGTCATC AGGGACTAAT GTCATTTGAA 
GGGTTTGCCA GGTTTCTGAT GGATAAAGAA AATTTTGCCT CAAAAAATGA TGAGTCACAG 
GAGAACATTA AAGAACTGCA GCTACCCCTC TCATACTATT ACATCGAATC TTCGCACAAT 
ACCTACCTCA CGGGCCATCA GCTCAAAGGA GAATCCTCGG TAGAACTCTA CAGCCAGGTC 
CTTTTGCAAG GCTGTCGAAG TGTAGAATTG GACTGCTGGG ACGGAGACGA TGGGATGCCC 
ATCATTTATC ATGGACATAC GCTGACAACC AAGATCCCCT TCAAGGAAGT GGTTGAAGCC 
ATTGATCGCA GTGCCTTCAT CAACTCTGAC CTGCCAATCA TCATATCGAT TGAGAACCAC 
TGTTCATTGC CTCAGCAACG AAAAATGGCA GAAATTTTCA AGACTGTGTT TGGAGAAAAG 
CTGGTGACTA AATTCTTATT TGAGACTGAT TTCTCAGATG ATCCAATGCT TCCTTCACCT 
GACCAACTCA GAAAGAAAGT TCTTCTTAAA AACAAGAAGC TAAAAGCCCA TCAGACGCCA 
GTGGATATCT TAAAGCAAAA GGCTCATCAG TTAGCATCTA TGCAAGTGCA GGCTTATAAT 
GGTGGGAATG CCAACCCCCG ACCTGCCAAT AATGAGGAAG AGGAAGATGA GGAGGACGAA 
TATGATTATG ACTATGAATC CCTTTCTGAT GCAGATGTCC TTACTGCCTC CCCTGCTCCT 
AACGGTCAGG AAGACAACAT TCTGGAAGAC AGACCTGAAA ATAAATCATG TAATGACAAG 
CTTCAGTTTG AATATAATGA AGAAATCCCA AAGAGGATAA AGAAAGCAGA TAACTCTGCT 
TGCAACAAAG GAAAGGTTTA TGATATGGAA CTGGGAGAAG AATTTTATCT TGATCAGAAT 
AAAAAGGAAA GCAGACAGAT TGCACCAGAG CTTTCTGACC TTGTAATCTA TTGTCAAGCA 
GTAAAATTTC CAGGACTGTC AACTCTAAAT GCATCTGGCT CTAGCAGAGG AAAAGAAAGG 
AAAAGCAGGA AGTCCATTTT TGGCAACAAT CCGGGCAGAA TGAGCCCAGG GGAGACAGCA 
TCATTTAACA AAACATCTGG AAAAAGTTCC TGTGAAGGCA TTCGACAGAC CTGGGAGGAA 
TCTTCTTCCC CTCTCAACCC AACCACGTCC CTCAGTGCTA TCATTAGAAC TCCCAAATGT 
TATCATATCT CGTCGCTGAA TGAAAATGCC GCCAAACGTC TGTGTCGCAG GTATTCTCAG 
AAACTGACCC AGCACACCGC CTGTCAGCTG CTGAGAACTT ACCCTGCTGC CACCCGCATC 
GACTCTTCCA ACCCGAACCC CCTCATGTTC TGGCTCCATG GGATACAGCT TGTGGCACTC 
AACTACCAGA CTGATGATCT CCCTTTACAT TTAAATGCTG CAATGTTTGA GGCAAATGGT 
GGTTGTGGTT ATGTATTGAA ACCTCCAGTT CTGTGGGACA AGAACTGCCC CATGTATCAG 
AAGTTTTCTC CACTAGAAAG AGATCTGGAC AGCATGGATC CTGCAGTCTA TTCTTTAACT 
ATTGTCTCTG GTCAGAATGT GTGCCCCAGT AATAGCATGG GAAGCCCGTG CATTGAAGTC 
GACGTCCTGG GCATGCCTCT GGACAGCTGC CATTTCCGCA CAAAGCCCAT CCATCGAAAC 
ACCCTGAACC CCATGTGGAA CGAGCAGTTT CTGTTCCACG TTCACTTCGA AGATCTTGTA 
TTTCTTCGTT TTGCAGTTGT GGAAAACAAT AGTTCAGCGG TAACTGCTCA GAGAATCATT 
CCACTGAAAG CTTTAAAACG AGGATATCGA CATCTTCAGC TGCGAAACCT TCACAATGAA 
GTCTTGGAGA TTTCTAGTTT ATTCATTAAC AGCAGAAGGA TGGAAGAAAA TTCCTCTGGC 
AATACCATGT CAGCCTCTTC GATGTTTAAT ACAGAAGAAA GAAAATGTTT GCAGACTCAC 
AGAGTCACGG TGCATGGGGT CCCAGGGCCA GAGCCCTTTA CCGTTTTCAC TATTAATGGA 
GGCACCAAGG CAAAGCAGCT TCTGCAGCAA ATTCTGACAA ATGAACAAGA CATCAAACCT 
GTTACCACAG ACTATTTTTT GATGGAAGAA AAATATTTTA TATCTAAAGA AAAGAATGAA 
TGTAGGAAAC AACCATTCCA GAGAGCCATT GGTCCAGAAG AGGAGATCAT GCAAATTTTA 
AGCAGCTGGT TTCCAGAAGA GGGATACATG GGCAGGATTG TCTTAAAAAC CCAGCAGGAA 
AACCTAGAAG AGAAAAACAT TGTTCAAGAT GACAAAGAGG TGATCTTGAG CTCAGAGGAG 
GAGAGTTTCT TTGTCCAAGT GCATGATGTT TCTCCAGAGC AACCTCGAAC AGTCATCAAA 
GCACCCCGCG TCAGCACTGC ACAGGATGTC ATTCAGCAGA CCTTATGCAA AGCCAAATAT 
TCCTACAGCA TCCTGAGCAA CCCCAATCCA AGCGACTATG TGCTTTTGGA AGAGGTGGTG 
AAAGACACTA CCAACAAGAA GACTACCACA CCAAAGTCCT CTCAGCGGGT CCTTCTGGAT 
CAGGAGTGTG TGTTTCAAGC CCAAAGCAAG TGGAAAGGTG CAGGAAAATT CATCCTTAAG 
CTAAAGGAGC AGGTGCAGGC ATCTCGAGAA GATAAAAAGA AAGGCATTTC TTTCGCAAGT 
GAACTCAAGA AGCTCACCAA GTCAACTAAA CAGCCCCGAG GACTTACATC ACCTTCTCAG 
CTCTTGACCT CAGAAAGTAT CCAAACCAAG GAGGAGAAAC CTGTGGGTGG CTTGTCCTCC 
AGTGACACAA TGGATTACCG ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCE1 ( XM_006717890.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717953.1 Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X7, mRNA.

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CloneID	OHu44368
Clone ID Related Accession (Same CDS sequence)	XM_006717890.2 , XM_006717890.3
Accession Version	XM_006717890.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006717890.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCAG AAGGAAGTGC AGCAGGAAGA GACTTTGCAG GAATGGAAGA GGTGAGGCAG 
CTCCACGTGA GATTCTGCAA AGGGATTAAG ATTTGGCACC AGGCTTGGTT TCTGTGCAGC 
CTCTTAGGCA GAGAGCCCCA GGAGAGGGAA GCAGGCTGTC AGCTGTGGCT TTGCACGCTC 
TCAGCTGTCT TGAAGGTTGG TTGGCTCTTC CCGCTCTCTG AGGTACCCAA TTTTACCTTG 
TTAAAGGATG GATGTGGATG CTGGAGGCTT AAAGAAGACC AGATCTACAA TGCAGTGAGA 
AGAGAAGAAA CAGAAAATAC AGTTGGATCT CTACTCCATT TCCTCACCAA GCTCCCAGCC 
TCCGAGACAG CCCATGGAAG GATAAGCGTT GGTCCATGCT TAAAGCAATG TGTCCGAGAC 
ACTGTATGTG AGTATCGCGC CACCCTCCAA AGGACTTCAA TATCGCAGTA CATCACCGGT 
TCTCTCCTAG AAGCAACCAC GTCTTTGGGA GCAAGAAGTG GCCTTCTCAG TACTTTTGGA 
GGATCCACTG GACGAATGAT GCTGAAAGAA CGCCAGCCAG GCCCCTCTGT GGCCAATTCC 
AATGCCCTCC CTTCAAGTTC AGCTGGGATC AGCAAGGAGC TGATCGATCT GCAGCCTCTC 
ATCCAGTTCC CAGAGGAAGT CGCCAGCATC CTGATGGAGC AAGAGCAGAC TATTTACCGC 
AGGGTCTTGC CAGTCGACTA CCTTTGCTTC TTAACACGGG ACTTGGGCAC TCCTGAATGC 
CAGAGCTCCT TGCCCTGCCT CAAAGCATCC ATCTCAGCGT CGATTCTTAC CACTCAGAAT 
GGAGAGCACA ATGCCCTTGA AGATCTGGTG ATGAGGTTTA ATGAGGTGAG CTCCTGGGTG 
ACATGGCTGA TCCTCACGGC AGGCTCCATG GAGGAGAAGC GAGAAGTCTT TTCATATTTG 
GTGCATGTGG CCAAATGCTG CTGGAACATG GGCAACTACA ACGCTGTCAT GGAGTTCTTG 
GCTGGCCTCA GGTCAAGAAA AGTTTTAAAA ATGTGGCAGT TCATGGACCA GTCTGATATT 
GAGACCATGA GGAGCCTGAA GGATGCTATG GCCCAGCATG AGTCCTCTTG TGAGTACAGA 
AAGGTGGTGA CACGTGCCCT GCACATCCCT GGCTGTAAGG TGGTTCCATT CTGTGGGGTG 
TTTCTGAAGG AGCTCTGTGA AGTGCTTGAC GGCGCCTCCG GTCTCATGAA GCTTTGCCCG 
CGGTACAATT CCCAAGAAGA AACTTTAGAG TTTGTAGCAG ATTACAGTGG ACAAGATAAT 
TTCTTACAAC GAGTGGGACA AAATGGCTTA AAGAATTCGG AGAAGGAGTC CACTGTCAAC 
AGCATCTTTC AGGTCATCCG GAGCTGCAAT CGAAGTCTGG AGACAGACGA GGAGGACAGC 
CCCAGTGAAG GAAACAGCTC CAGGAAAAGC TCCTTGAAGG ATAAAAGCCG ATGGCAGTTT 
ATAATTGGAG ATTTGTTGGA TTCAGACAAT GACATCTTTG AGCAATCCAA AGAATACGAC 
TCTCATGGTT CAGAGGACTC ACAGAAGGCC TTCGACCATG GGACGGAGCT CATCCCTTGG 
TACGTGCTGT CCATCCAAGC CGATGTGCAC CAGTTCCTGC TGCAGGGGGC CACGGTCATC 
CACTACGACC AGGACACACA CCTCTCTGCC CGCTGCTTCC TCCAGCTTCA GCCCGACAAT 
AGCACCTTGA CCTGGGTAAA GCCCACAACT GCCTCCCCAG CCAGCAGTAA AGCAAAACTT 
GGTGTACTTA ATAACACAGC TGAGCCTGGA AAATTCCCAC TACTGGGTAA TGCTGGATTA 
AGTAGCCTGA CGGAAGGGGT CTTGGATCTT TTTGCAGTGA AGGCTGTATA CATGGGCCAC 
CCTGGCATTG ATATACACAC TGTGTGTGTT CAGAACAAAC TGGGTAGCAT GTTCCTGTCA 
GAGACTGGTG TGACATTGCT CTATGGGCTT CAGACCACAG ACAACAGATT ATTGCACTTC 
GTGGCACCAA AGCACACAGC TAAAATGCTC TTCAGCGGAT TATTGGAACT CACTAGAGCT 
GTGAGAAAGA TGAGGAAATT CCCTGACCAA AGACAGCAGT GGCTGCGGAA ACAGTACGTC 
AGCCTTTATC AGGAGGATGG ACGGTATGAA GGCCCAACTT TGGCTCACGC TGTGGAGTTG 
TTTGGTGGCA GACGGTGGAG TGCTCGAAAC CCCAGCCCCG GAACATCAGC AAAGAATGCT 
GAGAAGCCCA ATATGCAGAG AAACAATACC CTGGGCATAA GCACTACCAA GAAAAAGAAG 
AAAATCCTCA TGAGGGGTGA GAGTGGAGAG GTAACTGACG ATGAGATGGC AACCCGAAAG 
GCCAAGATGC ACAAAGAGTG TCGAAGCCGG AGTGGTTCTG ATCCTCAAGA CATTAATGAA 
CAAGAAGAAT CAGAGGTGAA TGCCATCGCT AACCCTCCAA ACCCCCTCCC TTCCAGAAGA 
GCCCACTCTT TGACCACAGC TGGGTCCCCC AACTTGGCTG CCGGGACGTC ATCTCCCATC 
AGGCCAGTGT CCTCCCCTGT GCTGTCTTCT TCAAACAAGA GCCCATCCAG TGCTTGGAGC 
AGTAGTAGCT GGCACGGGCG GATCAAAGGC GGCATGAAGG GATTTCAGAG CTTCATGGTT 
TCAGATAGCA ACATGAGTTT TGTTGAATTT GTTGAGCTGT TCAAATCATT CAGTGTCAGG 
AGCCGCAAGG ACCTGAAGGA TCTGTTTGAT GTCTATGCAG TGCCCTGCAA CCGATCTGGC 
TCCGAGTCAG CCCCACTCTA CACCAACCTG ACAATTGATG AAAACACCAG CGATCTTCAG 
CCTGACCTAG ATCTGTTGAC CAGAAATGTC TCGGATTTGG GGTTGTTCAT TAAGAGTAAA 
CAGCAGCTAT CGGACAACCA GAGGCAGATA TCTGATGCCA TTGCTGCTGC AAGCATTGTG 
ACAAATGGCA CTGGGATTGA GAGCACATCT CTGGGCATTT TTGGGGTGGG CATACTTCAG 
CTCAACGATT TCCTCGTGAA TTGCCAAGGA GAACACTGCA CTTATGATGA AATCCTCAGC 
ATCATCCAGA AGTTCGAGCC TAGCATCAGT ATGTGTCATC AGGGACTAAT GTCATTTGAA 
GGGTTTGCCA GGTTTCTGAT GGATAAAGAA AATTTTGCCT CAAAAAATGA TGAGTCACAG 
GAGAACATTA AAGAACTGCA GCTACCCCTC TCATACTATT ACATCGAATC TTCGCACAAT 
ACCTACCTCA CGGGCCATCA GCTCAAAGGA GAATCCTCGG TAGAACTCTA CAGCCAGGTC 
CTTTTGCAAG GCTGTCGAAG TGTAGAATTG GACTGCTGGG ACGGAGACGA TGGGATGCCC 
ATCATTTATC ATGGACATAC GCTGACAACC AAGATCCCCT TCAAGGAAGT GGTTGAAGCC 
ATTGATCGCA GTGCCTTCAT CAACTCTGAC CTGCCAATCA TCATATCGAT TGAGAACCAC 
TGTTCATTGC CTCAGCAACG AAAAATGGCA GAAATTTTCA AGACTGTGTT TGGAGAAAAG 
CTGGTGACTA AATTCTTATT TGAGACTGAT TTCTCAGATG ATCCAATGCT TCCTTCACCT 
GACCAACTCA GAAAGAAAGT TCTTCTTAAA AACAAGAAGC TAAAAGCCCA TCAGACGCCA 
GTGGATATCT TAAAGCAAAA GGCTCATCAG TTAGCATCTA TGCAAGTGCA GGCTTATAAT 
GGTGGGAATG CCAACCCCCG ACCTGCCAAT AATGAGGAAG AGGAAGATGA GGAGGACGAA 
TATGATTATG ACTATGAATC CCTTTCTGAT GCAGATGTCC TTACTGCCTC CCCTGCTCCT 
AACGGTCAGG AAGACAACAT TCTGGAAGAC AGACCTGAAA ATAAATCATG TAATGACAAG 
CTTCAGTTTG AATATAATGA AGAAATCCCA AAGAGGATAA AGAAAGCAGA TAACTCTGCT 
TGCAACAAAG GAAAGGTTTA TGATATGGAA CTGGGAGAAG AATTTTATCT TGATCAGAAT 
AAAAAGGAAA GCAGACAGAT TGCACCAGAG CTTTCTGACC TTGTAATCTA TTGTCAAGCA 
GTAAAATTTC CAGGACTGTC AACTCTAAAT GCATCTGGCT CTAGCAGAGG AAAAGAAAGG 
AAAAGCAGGA AGTCCATTTT TGGCAACAAT CCGGGCAGAA TGAGCCCAGG GGAGACAGCA 
TCATTTAACA AAACATCTGG AAAAAGTTCC TGTGAAGGCA TTCGACAGAC CTGGGAGGAA 
TCTTCTTCCC CTCTCAACCC AACCACGTCC CTCAGTGCTA TCATTAGAAC TCCCAAATGT 
TATCATATCT CGTCGCTGAA TGAAAATGCC GCCAAACGTC TGTGTCGCAG GTATTCTCAG 
AAACTGACCC AGCACACCGC CTGTCAGCTG CTGAGAACTT ACCCTGCTGC CACCCGCATC 
GACTCTTCCA ACCCGAACCC CCTCATGTTC TGGCTCCATG GGATACAGCT TGTGGCACTC 
AACTACCAGA CTGATGATCT CCCTTTACAT TTAAATGCTG CAATGTTTGA GGCAAATGGT 
GGTTGTGGTT ATGTATTGAA ACCTCCAGTT CTGTGGGACA AGAACTGCCC CATGTATCAG 
AAGTTTTCTC CACTAGAAAG AGATCTGGAC AGCATGGATC CTGCAGTCTA TTCTTTAACT 
ATTGTCTCTG GTCAGAATGT GTGCCCCAGT AATAGCATGG GAAGCCCGTG CATTGAAGTC 
GACGTCCTGG GCATGCCTCT GGACAGCTGC CATTTCCGCA CAAAGCCCAT CCATCGAAAC 
ACCCTGAACC CCATGTGGAA CGAGCAGTTT CTGTTCCACG TTCACTTCGA AGATCTTGTA 
TTTCTTCGTT TTGCAGTTGT GGAAAACAAT AGTTCAGCGG TAACTGCTCA GAGAATCATT 
CCACTGAAAG CTTTAAAACG AGGATATCGA CATCTTCAGC TGCGAAACCT TCACAATGAA 
GTCTTGGAGA TTTCTAGTTT ATTCATTAAC AGCAGAAGGA TGGAAGAAAA TTCCTCTGGC 
AATACCATGT CAGCCTCTTC GATGTTTAAT ACAGAAGAAA GAAAATGTTT GCAGACTCAC 
AGAGTCACGG TGCATGGGGT CCCAGGGCCA GAGCCCTTTA CCGTTTTCAC TATTAATGGA 
GGCACCAAGG CAAAGCAGCT TCTGCAGCAA ATTCTGACAA ATGAACAAGA CATCAAACCT 
GTTACCACAG ACTATTTTTT GATGGAAGAA AAATATTTTA TATCTAAAGA AAAGAATGAA 
TGTAGGAAAC AACCATTCCA GAGAGCCATT GGTCCAGAAG AGGAGATCAT GCAAATTTTA 
AGCAGCTGGT TTCCAGAAGA GGGATACATG GGCAGGATTG TCTTAAAAAC CCAGCAGGAA 
AACCTAGAAG AGAAAAACAT TGTTCAAGAT GACAAAGAGG TGATCTTGAG CTCAGAGGAG 
GAGAGTTTCT TTGTCCAAGT GCATGATGTT TCTCCAGAGC AACCTCGAAC AGTCATCAAA 
GCACCCCGCG TCAGCACTGC ACAGGATGTC ATTCAGCAGA CCTTATGCAA AGCCAAATAT 
TCCTACAGCA TCCTGAGCAA CCCCAATCCA AGCGACTATG TGCTTTTGGA AGAGGTGGTG 
AAAGACACTA CCAACAAGAA GACTACCACA CCAAAGTCCT CTCAGCGGGT CCTTCTGGAT 
CAGGAGTGTG TGTTTCAAGC CCAAAGCAAG TGGAAAGGTG CAGGAAAATT CATCCTTAAG 
CTAAAGGAGC AGGTGCAGGC ATCTCGAGAA GATAAAAAGA AAGGCATTTC TTTCGCAAGT 
GAACTCAAGA AGCTCACCAA GTCAACTAAA CAGCCCCGAG GACTTACATC ACCTTCTCAG 
CTCTTGACCT CAGAAAGTAT CCAAACCAAG GAGGAGAAAC CTGTGGGTGG CTTGTCCTCC 
AGTGACACAA TGGATTACCG ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717953.1
CDS	23..6049
Translation	MVSEGSAAGRDFAGMEEVRQLHVRFCKGIKIWHQAWFLCSLLGREPQEREAGCQLWLCTLSAVLKVGWLFPLSEVPNFTLLKDGCGCWRLKEDQIYNAVRREETENTVGSLLHFLTKLPASETAHGRISVGPCLKQCVRDTVCEYRATLQRTSISQYITGSLLEATTSLGARSGLLSTFGGSTGRMMLKERQPGPSVANSNALPSSSAGISKELIDLQPLIQFPEEVASILMEQEQTIYRRVLPVDYLCFLTRDLGTPECQSSLPCLKASISASILTTQNGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAKCCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSCEYRKVVTRALHIPGCKVVPFCGVFLKELCEVLDGASGLMKLCPRYNSQEETLEFVADYSGQDNFLQRVGQNGLKNSEKESTVNSIFQVIRSCNRSLETDEEDSPSEGNSSRKSSLKDKSRWQFIIGDLLDSDNDIFEQSKEYDSHGSEDSQKAFDHGTELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWVKPTTASPASSKAKLGVLNNTAEPGKFPLLGNAGLSSLTEGVLDLFAVKAVYMGHPGIDIHTVCVQNKLGSMFLSETGVTLLYGLQTTDNRLLHFVAPKHTAKMLFSGLLELTRAVRKMRKFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRRWSARNPSPGTSAKNAEKPNMQRNNTLGISTTKKKKKILMRGESGEVTDDEMATRKAKMHKECRSRSGSDPQDINEQEESEVNAIANPPNPLPSRRAHSLTTAGSPNLAAGTSSPIRPVSSPVLSSSNKSPSSAWSSSSWHGRIKGGMKGFQSFMVSDSNMSFVEFVELFKSFSVRSRKDLKDLFDVYAVPCNRSGSESAPLYTNLTIDENTSDLQPDLDLLTRNVSDLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSIIQKFEPSISMCHQGLMSFEGFARFLMDKENFASKNDESQENIKELQLPLSYYYIESSHNTYLTGHQLKGESSVELYSQVLLQGCRSVELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFINSDLPIIISIENHCSLPQQRKMAEIFKTVFGEKLVTKFLFETDFSDDPMLPSPDQLRKKVLLKNKKLKAHQTPVDILKQKAHQLASMQVQAYNGGNANPRPANNEEEEDEEDEYDYDYESLSDADVLTASPAPNGQEDNILEDRPENKSCNDKLQFEYNEEIPKRIKKADNSACNKGKVYDMELGEEFYLDQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNASGSSRGKERKSRKSIFGNNPGRMSPGETASFNKTSGKSSCEGIRQTWEESSSPLNPTTSLSAIIRTPKCYHISSLNENAAKRLCRRYSQKLTQHTACQLLRTYPAATRIDSSNPNPLMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVLKPPVLWDKNCPMYQKFSPLERDLDSMDPAVYSLTIVSGQNVCPSNSMGSPCIEVDVLGMPLDSCHFRTKPIHRNTLNPMWNEQFLFHVHFEDLVFLRFAVVENNSSAVTAQRIIPLKALKRGYRHLQLRNLHNEVLEISSLFINSRRMEENSSGNTMSASSMFNTEERKCLQTHRVTVHGVPGPEPFTVFTINGGTKAKQLLQQILTNEQDIKPVTTDYFLMEEKYFISKEKNECRKQPFQRAIGPEEEIMQILSSWFPEEGYMGRIVLKTQQENLEEKNIVQDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILSNPNPSDYVLLEEVVKDTTNKKTTTPKSSQRVLLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKKKGISFASELKKLTKSTKQPRGLTSPSQLLTSESIQTKEEKPVGGLSSSDTMDYRQ

Target ORF information:

RefSeq Version	XM_006717890.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholipase C epsilon 1 (PLCE1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006717890.3

ORF Insert Sequence:

ATGGTTTCAG AAGGAAGTGC AGCAGGAAGA GACTTTGCAG GAATGGAAGA GGTGAGGCAG 
CTCCACGTGA GATTCTGCAA AGGGATTAAG ATTTGGCACC AGGCTTGGTT TCTGTGCAGC 
CTCTTAGGCA GAGAGCCCCA GGAGAGGGAA GCAGGCTGTC AGCTGTGGCT TTGCACGCTC 
TCAGCTGTCT TGAAGGTTGG TTGGCTCTTC CCGCTCTCTG AGGTACCCAA TTTTACCTTG 
TTAAAGGATG GATGTGGATG CTGGAGGCTT AAAGAAGACC AGATCTACAA TGCAGTGAGA 
AGAGAAGAAA CAGAAAATAC AGTTGGATCT CTACTCCATT TCCTCACCAA GCTCCCAGCC 
TCCGAGACAG CCCATGGAAG GATAAGCGTT GGTCCATGCT TAAAGCAATG TGTCCGAGAC 
ACTGTATGTG AGTATCGCGC CACCCTCCAA AGGACTTCAA TATCGCAGTA CATCACCGGT 
TCTCTCCTAG AAGCAACCAC GTCTTTGGGA GCAAGAAGTG GCCTTCTCAG TACTTTTGGA 
GGATCCACTG GACGAATGAT GCTGAAAGAA CGCCAGCCAG GCCCCTCTGT GGCCAATTCC 
AATGCCCTCC CTTCAAGTTC AGCTGGGATC AGCAAGGAGC TGATCGATCT GCAGCCTCTC 
ATCCAGTTCC CAGAGGAAGT CGCCAGCATC CTGATGGAGC AAGAGCAGAC TATTTACCGC 
AGGGTCTTGC CAGTCGACTA CCTTTGCTTC TTAACACGGG ACTTGGGCAC TCCTGAATGC 
CAGAGCTCCT TGCCCTGCCT CAAAGCATCC ATCTCAGCGT CGATTCTTAC CACTCAGAAT 
GGAGAGCACA ATGCCCTTGA AGATCTGGTG ATGAGGTTTA ATGAGGTGAG CTCCTGGGTG 
ACATGGCTGA TCCTCACGGC AGGCTCCATG GAGGAGAAGC GAGAAGTCTT TTCATATTTG 
GTGCATGTGG CCAAATGCTG CTGGAACATG GGCAACTACA ACGCTGTCAT GGAGTTCTTG 
GCTGGCCTCA GGTCAAGAAA AGTTTTAAAA ATGTGGCAGT TCATGGACCA GTCTGATATT 
GAGACCATGA GGAGCCTGAA GGATGCTATG GCCCAGCATG AGTCCTCTTG TGAGTACAGA 
AAGGTGGTGA CACGTGCCCT GCACATCCCT GGCTGTAAGG TGGTTCCATT CTGTGGGGTG 
TTTCTGAAGG AGCTCTGTGA AGTGCTTGAC GGCGCCTCCG GTCTCATGAA GCTTTGCCCG 
CGGTACAATT CCCAAGAAGA AACTTTAGAG TTTGTAGCAG ATTACAGTGG ACAAGATAAT 
TTCTTACAAC GAGTGGGACA AAATGGCTTA AAGAATTCGG AGAAGGAGTC CACTGTCAAC 
AGCATCTTTC AGGTCATCCG GAGCTGCAAT CGAAGTCTGG AGACAGACGA GGAGGACAGC 
CCCAGTGAAG GAAACAGCTC CAGGAAAAGC TCCTTGAAGG ATAAAAGCCG ATGGCAGTTT 
ATAATTGGAG ATTTGTTGGA TTCAGACAAT GACATCTTTG AGCAATCCAA AGAATACGAC 
TCTCATGGTT CAGAGGACTC ACAGAAGGCC TTCGACCATG GGACGGAGCT CATCCCTTGG 
TACGTGCTGT CCATCCAAGC CGATGTGCAC CAGTTCCTGC TGCAGGGGGC CACGGTCATC 
CACTACGACC AGGACACACA CCTCTCTGCC CGCTGCTTCC TCCAGCTTCA GCCCGACAAT 
AGCACCTTGA CCTGGGTAAA GCCCACAACT GCCTCCCCAG CCAGCAGTAA AGCAAAACTT 
GGTGTACTTA ATAACACAGC TGAGCCTGGA AAATTCCCAC TACTGGGTAA TGCTGGATTA 
AGTAGCCTGA CGGAAGGGGT CTTGGATCTT TTTGCAGTGA AGGCTGTATA CATGGGCCAC 
CCTGGCATTG ATATACACAC TGTGTGTGTT CAGAACAAAC TGGGTAGCAT GTTCCTGTCA 
GAGACTGGTG TGACATTGCT CTATGGGCTT CAGACCACAG ACAACAGATT ATTGCACTTC 
GTGGCACCAA AGCACACAGC TAAAATGCTC TTCAGCGGAT TATTGGAACT CACTAGAGCT 
GTGAGAAAGA TGAGGAAATT CCCTGACCAA AGACAGCAGT GGCTGCGGAA ACAGTACGTC 
AGCCTTTATC AGGAGGATGG ACGGTATGAA GGCCCAACTT TGGCTCACGC TGTGGAGTTG 
TTTGGTGGCA GACGGTGGAG TGCTCGAAAC CCCAGCCCCG GAACATCAGC AAAGAATGCT 
GAGAAGCCCA ATATGCAGAG AAACAATACC CTGGGCATAA GCACTACCAA GAAAAAGAAG 
AAAATCCTCA TGAGGGGTGA GAGTGGAGAG GTAACTGACG ATGAGATGGC AACCCGAAAG 
GCCAAGATGC ACAAAGAGTG TCGAAGCCGG AGTGGTTCTG ATCCTCAAGA CATTAATGAA 
CAAGAAGAAT CAGAGGTGAA TGCCATCGCT AACCCTCCAA ACCCCCTCCC TTCCAGAAGA 
GCCCACTCTT TGACCACAGC TGGGTCCCCC AACTTGGCTG CCGGGACGTC ATCTCCCATC 
AGGCCAGTGT CCTCCCCTGT GCTGTCTTCT TCAAACAAGA GCCCATCCAG TGCTTGGAGC 
AGTAGTAGCT GGCACGGGCG GATCAAAGGC GGCATGAAGG GATTTCAGAG CTTCATGGTT 
TCAGATAGCA ACATGAGTTT TGTTGAATTT GTTGAGCTGT TCAAATCATT CAGTGTCAGG 
AGCCGCAAGG ACCTGAAGGA TCTGTTTGAT GTCTATGCAG TGCCCTGCAA CCGATCTGGC 
TCCGAGTCAG CCCCACTCTA CACCAACCTG ACAATTGATG AAAACACCAG CGATCTTCAG 
CCTGACCTAG ATCTGTTGAC CAGAAATGTC TCGGATTTGG GGTTGTTCAT TAAGAGTAAA 
CAGCAGCTAT CGGACAACCA GAGGCAGATA TCTGATGCCA TTGCTGCTGC AAGCATTGTG 
ACAAATGGCA CTGGGATTGA GAGCACATCT CTGGGCATTT TTGGGGTGGG CATACTTCAG 
CTCAACGATT TCCTCGTGAA TTGCCAAGGA GAACACTGCA CTTATGATGA AATCCTCAGC 
ATCATCCAGA AGTTCGAGCC TAGCATCAGT ATGTGTCATC AGGGACTAAT GTCATTTGAA 
GGGTTTGCCA GGTTTCTGAT GGATAAAGAA AATTTTGCCT CAAAAAATGA TGAGTCACAG 
GAGAACATTA AAGAACTGCA GCTACCCCTC TCATACTATT ACATCGAATC TTCGCACAAT 
ACCTACCTCA CGGGCCATCA GCTCAAAGGA GAATCCTCGG TAGAACTCTA CAGCCAGGTC 
CTTTTGCAAG GCTGTCGAAG TGTAGAATTG GACTGCTGGG ACGGAGACGA TGGGATGCCC 
ATCATTTATC ATGGACATAC GCTGACAACC AAGATCCCCT TCAAGGAAGT GGTTGAAGCC 
ATTGATCGCA GTGCCTTCAT CAACTCTGAC CTGCCAATCA TCATATCGAT TGAGAACCAC 
TGTTCATTGC CTCAGCAACG AAAAATGGCA GAAATTTTCA AGACTGTGTT TGGAGAAAAG 
CTGGTGACTA AATTCTTATT TGAGACTGAT TTCTCAGATG ATCCAATGCT TCCTTCACCT 
GACCAACTCA GAAAGAAAGT TCTTCTTAAA AACAAGAAGC TAAAAGCCCA TCAGACGCCA 
GTGGATATCT TAAAGCAAAA GGCTCATCAG TTAGCATCTA TGCAAGTGCA GGCTTATAAT 
GGTGGGAATG CCAACCCCCG ACCTGCCAAT AATGAGGAAG AGGAAGATGA GGAGGACGAA 
TATGATTATG ACTATGAATC CCTTTCTGAT GCAGATGTCC TTACTGCCTC CCCTGCTCCT 
AACGGTCAGG AAGACAACAT TCTGGAAGAC AGACCTGAAA ATAAATCATG TAATGACAAG 
CTTCAGTTTG AATATAATGA AGAAATCCCA AAGAGGATAA AGAAAGCAGA TAACTCTGCT 
TGCAACAAAG GAAAGGTTTA TGATATGGAA CTGGGAGAAG AATTTTATCT TGATCAGAAT 
AAAAAGGAAA GCAGACAGAT TGCACCAGAG CTTTCTGACC TTGTAATCTA TTGTCAAGCA 
GTAAAATTTC CAGGACTGTC AACTCTAAAT GCATCTGGCT CTAGCAGAGG AAAAGAAAGG 
AAAAGCAGGA AGTCCATTTT TGGCAACAAT CCGGGCAGAA TGAGCCCAGG GGAGACAGCA 
TCATTTAACA AAACATCTGG AAAAAGTTCC TGTGAAGGCA TTCGACAGAC CTGGGAGGAA 
TCTTCTTCCC CTCTCAACCC AACCACGTCC CTCAGTGCTA TCATTAGAAC TCCCAAATGT 
TATCATATCT CGTCGCTGAA TGAAAATGCC GCCAAACGTC TGTGTCGCAG GTATTCTCAG 
AAACTGACCC AGCACACCGC CTGTCAGCTG CTGAGAACTT ACCCTGCTGC CACCCGCATC 
GACTCTTCCA ACCCGAACCC CCTCATGTTC TGGCTCCATG GGATACAGCT TGTGGCACTC 
AACTACCAGA CTGATGATCT CCCTTTACAT TTAAATGCTG CAATGTTTGA GGCAAATGGT 
GGTTGTGGTT ATGTATTGAA ACCTCCAGTT CTGTGGGACA AGAACTGCCC CATGTATCAG 
AAGTTTTCTC CACTAGAAAG AGATCTGGAC AGCATGGATC CTGCAGTCTA TTCTTTAACT 
ATTGTCTCTG GTCAGAATGT GTGCCCCAGT AATAGCATGG GAAGCCCGTG CATTGAAGTC 
GACGTCCTGG GCATGCCTCT GGACAGCTGC CATTTCCGCA CAAAGCCCAT CCATCGAAAC 
ACCCTGAACC CCATGTGGAA CGAGCAGTTT CTGTTCCACG TTCACTTCGA AGATCTTGTA 
TTTCTTCGTT TTGCAGTTGT GGAAAACAAT AGTTCAGCGG TAACTGCTCA GAGAATCATT 
CCACTGAAAG CTTTAAAACG AGGATATCGA CATCTTCAGC TGCGAAACCT TCACAATGAA 
GTCTTGGAGA TTTCTAGTTT ATTCATTAAC AGCAGAAGGA TGGAAGAAAA TTCCTCTGGC 
AATACCATGT CAGCCTCTTC GATGTTTAAT ACAGAAGAAA GAAAATGTTT GCAGACTCAC 
AGAGTCACGG TGCATGGGGT CCCAGGGCCA GAGCCCTTTA CCGTTTTCAC TATTAATGGA 
GGCACCAAGG CAAAGCAGCT TCTGCAGCAA ATTCTGACAA ATGAACAAGA CATCAAACCT 
GTTACCACAG ACTATTTTTT GATGGAAGAA AAATATTTTA TATCTAAAGA AAAGAATGAA 
TGTAGGAAAC AACCATTCCA GAGAGCCATT GGTCCAGAAG AGGAGATCAT GCAAATTTTA 
AGCAGCTGGT TTCCAGAAGA GGGATACATG GGCAGGATTG TCTTAAAAAC CCAGCAGGAA 
AACCTAGAAG AGAAAAACAT TGTTCAAGAT GACAAAGAGG TGATCTTGAG CTCAGAGGAG 
GAGAGTTTCT TTGTCCAAGT GCATGATGTT TCTCCAGAGC AACCTCGAAC AGTCATCAAA 
GCACCCCGCG TCAGCACTGC ACAGGATGTC ATTCAGCAGA CCTTATGCAA AGCCAAATAT 
TCCTACAGCA TCCTGAGCAA CCCCAATCCA AGCGACTATG TGCTTTTGGA AGAGGTGGTG 
AAAGACACTA CCAACAAGAA GACTACCACA CCAAAGTCCT CTCAGCGGGT CCTTCTGGAT 
CAGGAGTGTG TGTTTCAAGC CCAAAGCAAG TGGAAAGGTG CAGGAAAATT CATCCTTAAG 
CTAAAGGAGC AGGTGCAGGC ATCTCGAGAA GATAAAAAGA AAGGCATTTC TTTCGCAAGT 
GAACTCAAGA AGCTCACCAA GTCAACTAAA CAGCCCCGAG GACTTACATC ACCTTCTCAG 
CTCTTGACCT CAGAAAGTAT CCAAACCAAG GAGGAGAAAC CTGTGGGTGG CTTGTCCTCC 
AGTGACACAA TGGATTACCG ACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PLCE1
Entrez Gene ID	51196
Full Name	phospholipase C epsilon 1
Synonyms	NPHS3,PLCE,PPLC
General protein information	Preferred Names phospholipase C epsilon 1 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 PLC-epsilon-1 pancreas-enriched phospholipase C phosphoinositide phospholipase C phosphoinositide phospholipase C-epsilon-1 phosphoinositide-specific phospholipase C epsilon-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q23.33
Summary	This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009].
Disorder MIM:	608414
Disorder Html:	Nephrotic syndrome, type 3, 610725 (3)

Homo sapiens (human)	PLCE1	NP_057425.3
Gallus gallus (chicken)	LOC425224	XP_004942210.1
Danio rerio (zebrafish)	plce1	NP_001155125.1
Bos taurus (cattle)	PLCE1	NP_001192781.1
	PLCE1	XP_002805823.1
Mus musculus (house mouse)	Plce1	NP_062534.2
Rattus norvegicus (Norway rat)	Plce1	NP_446210.1
Caenorhabditis elegans (roundworm)	plc-1	NP_001123141.1
Canis lupus familiaris (dog)	PLCE1	NP_001130037.1
Pan troglodytes (chimpanzee)	PLCE1	XP_001149565.1
Xenopus tropicalis (tropical clawed frog)	plce1	XP_002937222.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PLCE1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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