plce1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following plce1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plce1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa45567 XM_018095634.1
Latest version!
Xenopus tropicalis phospholipase C epsilon 1 (plce1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OXa62378 XM_031905944.1
Latest version!
Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa62378 XM_031905943.1
Latest version!
Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa62379 XM_031905945.1
Latest version!
Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa62380 XM_031905946.1
Latest version!
Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa62381 XM_031905947.1
Latest version!
Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa62382 XM_031905948.1
Latest version!
Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa62383 XM_031905949.1
Latest version!
Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa62384 XM_031905950.1
Latest version!
Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa62385 XM_031905951.1
Latest version!
Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa45567
    Clone ID Related Accession (Same CDS sequence) XM_018095634.1
    Accession Version XM_018095634.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5343bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGACCAAGG ACAATATTTT TTCTATAGAA AAGTGCCCCA AGCCACCCCA GCCCAGTGTC 
    AACACCCCAA AATCTTCAGA ATCTGAGAAG AGTCACACTA GGCTGGAGGT TAGCTCCTGG
    GTCACATGGC TGATTCTAAC GGCAGGATCT ATGGAGGAGA AACGAGAAGT TTTCTCACAC
    GTCGTTCACA TTGCAAAGTG TTGCTGGAAC ATGGGCAATT ACAACGCTGT CATGGAATTC
    CTGGCAGGCC TCAGGACAAG AAAAGTTCTA AAAATGTGGC AATTTATGGA TCAGTCCGAC
    ATTGAAACGA TGCGCAGCTT GAAAGATGCA ATGGCACAGC ATGAATCCTC ATCCGAGTAC
    AGGAAAGTGG TGAACAGGGC CCTAAATATA CCTGGCTGTA AGGTGGTTCC TTTTTGTGGG
    GTCTTTCTAA AGGAACTCTG TGAAGTCCTT GATGGAGCAG CGAGTATCAT CAGTCTGTGT
    CCTCAGTACG ATGCTCAGAG TGAAACACTG GAGTTTGTTT CAGATTACAA TGGGCAAGAC
    AACTTTCTCC AGCGAATAGG GAAAGATGGA TTAAAGAACA CAGAGAAAGA GTCCACTGTG
    AATAGTATCA TGCAGACAAT TCGTAGCTGT AACCGTAGCT TGGAATCTGA AGAAGGAGAA
    GACAATCTCA GCGATGCCGG GGGTATCAGG AAAAGCACAG TGATGGACAG AACTAGATTT
    CAGTTTATTA TGGGAGATTT ATCTGACTCT GAAAGTGATA TCTTTGAACA ATCAAAAGAG
    TGGGACTTGC ATAGGAGGGA AGAGCAACAG AAGGCATTCA GCCATGGAAC AGAGCTAATT
    CCCTGGTATG TGTTATCCAT GAGAGCAGAT GTCTATCAGT TTCTTCAGCA AGGAGTGACT
    GTCATCCGCT ATGACCAGGA GACACACATT TCTGTTCGTT GTTTCCTTCA ACTCCAACCA
    GACAACTCCA CATTGACTTG GACAAAACCA ACCACAACGT GTCTTGCAAA CACTAAAAAT
    AAATTAGGAA CTGTAAGTAG CAGTGCGGAG ATTAAATTTC AGTTCCTTGC CAATGCTGGA
    ATGAATGGAC TGGCCGAAGG ATTCTTGGAC TTATTCTCTG TAAAGGCAGT TTACATGGGA
    CATCCGGGAA TTGATATGCA CACTGTGTGT GTTCAAAACA AACTATGTAA TATGAACCTG
    GAAGAAAATG GTGTCACATT GCTTTATGGG CTGCACACTA CTGACAACAA GTTGCTGCAC
    TTTGTAGCTC CGAAATACAC AGCCAGGACA TTGTATGATG GACTCCTGGA GTTAACCAAA
    GCAGTAAGGA AGATAAAACG CTTTCCTGAT CAGAGATTAC AGTGGTTAAG GAAGCAGTAT
    GTTAGTCTCT ACCAGGAGGA AAACAGATTT GAAGGACCAG CTTTGGCACA AGCTATTGAG
    CTGTTTGGAG GGCGACGCTG GAGTACGAGA AACACGAGCA CAGGGACCTT AACCAAAAGC
    ACAGAGAAAC CAAACGTGCA GAGGAATAAC ACTCTCGGCA TCAACACTGC CAAGAAGAAG
    AAGAAAGTGC TTATGAGGGG CGAGAGTGGG GATGCAGCCG ATGATGAGAT GGCAACCCGC
    AAAACAAAAA GCTGCAAGGA AAGTCGCAGT AGAAGTGGCT CTGATCCTCC AGAAATCGAT
    GAGCAGGAAG AGCAAGATCT GAATATTATT GCTGGATATT CACCATCTCA AATGCTCCCA
    TCCCGAAGAG CTCATTCTAT GTCGACTTCT GGTTCTCCCA ACTTGACACC CGGGCCATCT
    ATTCCACTGC GACCTGCCTC CTCTCCTATT TTGTCCAACT CAAACAAACC CCAGTCCAAT
    ACGTGGAGCA GTTCAAGTTG GCATGGAAGA GTTAAAGGTG GGATGAAAGG CTTCCAATCA
    TTCATGGTTT CGGACAGCAA CATGAACTTT ACTGAATTTG TAGAGCTTTT CAAATCTTTT
    AGCGTAAGAA GTCGGAAAGA CCTGAAGGAC ATTTTTGATG TGTATGCTGT GGCCTGCAAT
    CGGTCAGGTG CAGAATCAGT CCCTCTCTAC ACTAATTTGA CAATTGATGA GAATGTAGTT
    GGAGTCCAAC CTGATTTAGA TTTATTGACC AGGAATGTGT CCGACTTGGG TTTATTCATT
    CGCAGCAGAC AGCAACTGTC TGAGAACCAA AGGCAAATCT CAGATGCCAT TGCAGCTGCC
    AGCATTGTAA CTAATGGAAC TGGAGTAGAA AGCACATCCC TGGGCATATT TGGAATGGGA
    ATACTGCAGT TTAATGATTT CCTGGTGAAT TGCCAGGGTG AACATTACAC CTATGATGAA
    GTTCTTAGCA TTATTCAGAA ATTTGAACCA AGTATCAGTA TGCGCCAGCA GGGGCTGATG
    TCATTTGAAG GATTTGCAAG GTTCCTGATG GATAAAGACA ATTTTGCATC AAGAAATGAT
    GAGTCTCAGG TAAACACAGA AGAACTTCAG CTGCCCCTTT CCTACTATTT CATTGAGTCT
    TCACACAACA CCTACCTTAC AGGGCACCAG CTAAAAGGAG AGTCTTCAGT TGAGCTGTAC
    AGCCAGGTCC TTTTGCAGGG GTGCCGAAGT GTTGAATTAG ACTGCTGGGA TGGAGATGAC
    GGCATGCCTA TCATTTATCA TGGACATACA CTAACTACTA AAATTCCCTT TAAGGATGTC
    ATTGAAGCTA TTGGCCGAAG TGCATTTATT ACCTCTGAAA TGCCAATCGT ACTGTCCATA
    GAAAACCATT GCTCCCTACC ACAGCAGCGT AAAATGGCAG ATATATTTAA GAATGTCTTT
    GGTGAAAAAC TGGTGACAAA GTTCCTGTTT GAAAGTGACT TTTCAGATGA TCCGATGTTG
    CCTTCTCCAT GGCAATTGAG AAATAAAGTG CTTCTGAAAA ATAAAAAGCT GAAGGCACAT
    CAAACCCCGG TGGATATACT GAAGCAGAAG GCACATCAGC TGGCTAGCAT GCAAGCACAA
    GCAAGCAATG GTAGCCAAAT GGTAAGCCCT AGCACAAACA ATGAAGAAGA AGAAGATGAA
    GAAGATGAAT ATGATTATGA CTATGAGTCT CTGTCAGATG CCGATGTCCT TAATGCTTCC
    CCTGCTCCTA ACAGCCAGGA AGATAATATT CTTGATGACC GATCTGAAAC TAAAACCAAC
    AGCGACAAAC TGCAATTTGA ATATAATGAG GAAGCTTCAA AGCGAATCAA AAAGACAGAT
    GGCAGCAGTA TTAACACTAA AGGAAAGGTC TATGACATGG AGTTGGGTGA GGAATTCTAT
    CTTCCACAAA ACAAAAAAGA GAGCAGGCAA ATTGCACCTG AATTGTCAGA CCTTATTATC
    TATTGCCAAG CTGTCAAATT CCCAGGCCTT ACAACATTAA ACCCATGTGG ATCTGGCAGA
    GGGAAGGAAA GAAAAAGCAG GAAGTCCATT TTTGGCAACA ACCCTGGCAG AACAAGTCCT
    GGGGAACCAA CAGCACTTGC CAAAACATCT GGAAAAGGTA CAAATGAAAC ACGGCAGAGC
    TGGGAAGAGC CATGTTCTCC TCCCTTTAAC CCCAGCACTT CCCTCAGTGC AATAATCAGG
    ACACCCAGGT GCTACCATAT CTCTTCCCTA AATGAGAACG CAGCCAAGCG CTTGTGCCGA
    AGGTATTCTC AGAAACTCAT TCAACATACC GCATATCAAC TGCTCCGAAC ATATCCCGCC
    GCCACTCGGA TAGACTCTTC CAACCCACAT CCTCTTATTT TCTGGCTTCA TGGTGTACAG
    TTGGTTGCTC TAAACTACCA AACTGATGAT CTCCCCTTAC ATCTAAACGC CGCCATGTTT
    GAAGCAAATG GTGGTTGCGG ATATGTGTTA AAGCCCCCCG TGCAGTGGGA TAGGACCTGC
    CCAATGTACC AATTATTCTC ACCATTAGAA CGAGATTTAG AAAATATGGA ACCAGCAATA
    TATTCTTTGA CGATAGTCTC TGGGCAAAAT GTGTGTCCAG GTAACAGCTC AGGCAGCCCC
    TGCATTGAAA TTGATGTCTT GGGTATGCCA GTAGACAGCT GCCATTTTCG GACCAAACCA
    ATCCATCGCA ATACCTTAAA TCCCATGTGG AATGAACAAT TCTTATTCCG AATATACTTT
    GAGGATCTTG TGTTTCTTCG GTTTGCTGTG GTGGAAAACA ACAGTTCTGC TGTAACTGCC
    CAAAGAATAA TTAATCTTAA AGCTTTAAAG AGAGGTAACA GACACCTTCA GCTAAGAAAT
    TTACATAATG AGCCCTTAGA GGTTTCTACC TTATTTATAA ATAGCAGGAG AATGGAAGAA
    ATCCCTAATG GAAATACCTT ACCCGCCTCC TTGTTTTTCA GCTCAGAAGA AAGAAAGACT
    CCAGCCACAT TTAAAGCTAC AGTACATGGG ATTCCTGGGC CAGAACCATT TACAGTGTTT
    GAAATCAGTC TTGGGACAAC TGCAAAGCAA CTTCTTGATC ATATTTTGGC TACTATACAA
    GGCAATGAAG CAGACATCAC TGACTATTTT TTGATGGAGG AAAAATGCTT TATATCTAAA
    GACAAAAATG AATGCAAGAA ATTGCCGTTC CAGAGAGTGA TTAGCCCAGA AGAGGATATC
    CTGCAAATTT TGAATAGCTG GTTCCCAGAA GAAGGCTACG TGGGGAGAAT TGTTCTTAAA
    ACACGAGAGG AGAACATGAA TGACAAAAAT GTTCAAGAGG ACAAAGAAGA AAAAGAGGTG
    ACCGTGAGCT CCGATGAGGA GACTTTCTTT GTCCAGGTCC ATGATGTTTC TCCTGAACAG
    CCAAGAACGG TTATCAAAGC TTTACGATTC AGTACAGCGC AGGAAGTTAT CCAGCAGGCT
    TTATGCAAGG CTAAATACTC ATACAGCATC CTGAGCAACC CTAATCCTAG TGATTATGTG
    CTTCTGGAGG AAGTCATCAA AGAAGCTTCA AATAAGAAGT CTTCTACCTC CAAAACAATT
    CAGAGGGTTC TCTTGGATCA AGAGTGTGTA TTCCAGGCAC AGAGCAAGTG GAAGGGAGCA
    GGGAAATTCA TTCTTAAACT GAAGGAACAA GTCCAGGCGG CAAGAGACGA CAAGCGCAAA
    GGTCTGTCTA TAGCCAGTGA ACTGAAGAAG CTAGCAAAGT CCAGCCGGCA ATCTCGGAAC
    TTCACTTTGT CACCTCAGAT ATTTTTATCA GAAGATGCCT GTGAAAGCCC TGAACCTACA
    AAAAGCAAAA CGCAGGAAAA GCCTTCGGCA GATAATGAAG TAAATGTGCA TCCAACCTGC
    TGCAGGAGGT CTTCTGCTGC CTGGTACTTT CTGTTGCTGA AGAACTGGAA GCTGCCAAAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951123.1
    CDS1..5343
    Translation

    Target ORF information:

    RefSeq Version XM_018095634.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C epsilon 1 (plce1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095634.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGACCAAGG ACAATATTTT TTCTATAGAA AAGTGCCCCA AGCCACCCCA GCCCAGTGTC 
    AACACCCCAA AATCTTCAGA ATCTGAGAAG AGTCACACTA GGCTGGAGGT TAGCTCCTGG
    GTCACATGGC TGATTCTAAC GGCAGGATCT ATGGAGGAGA AACGAGAAGT TTTCTCACAC
    GTCGTTCACA TTGCAAAGTG TTGCTGGAAC ATGGGCAATT ACAACGCTGT CATGGAATTC
    CTGGCAGGCC TCAGGACAAG AAAAGTTCTA AAAATGTGGC AATTTATGGA TCAGTCCGAC
    ATTGAAACGA TGCGCAGCTT GAAAGATGCA ATGGCACAGC ATGAATCCTC ATCCGAGTAC
    AGGAAAGTGG TGAACAGGGC CCTAAATATA CCTGGCTGTA AGGTGGTTCC TTTTTGTGGG
    GTCTTTCTAA AGGAACTCTG TGAAGTCCTT GATGGAGCAG CGAGTATCAT CAGTCTGTGT
    CCTCAGTACG ATGCTCAGAG TGAAACACTG GAGTTTGTTT CAGATTACAA TGGGCAAGAC
    AACTTTCTCC AGCGAATAGG GAAAGATGGA TTAAAGAACA CAGAGAAAGA GTCCACTGTG
    AATAGTATCA TGCAGACAAT TCGTAGCTGT AACCGTAGCT TGGAATCTGA AGAAGGAGAA
    GACAATCTCA GCGATGCCGG GGGTATCAGG AAAAGCACAG TGATGGACAG AACTAGATTT
    CAGTTTATTA TGGGAGATTT ATCTGACTCT GAAAGTGATA TCTTTGAACA ATCAAAAGAG
    TGGGACTTGC ATAGGAGGGA AGAGCAACAG AAGGCATTCA GCCATGGAAC AGAGCTAATT
    CCCTGGTATG TGTTATCCAT GAGAGCAGAT GTCTATCAGT TTCTTCAGCA AGGAGTGACT
    GTCATCCGCT ATGACCAGGA GACACACATT TCTGTTCGTT GTTTCCTTCA ACTCCAACCA
    GACAACTCCA CATTGACTTG GACAAAACCA ACCACAACGT GTCTTGCAAA CACTAAAAAT
    AAATTAGGAA CTGTAAGTAG CAGTGCGGAG ATTAAATTTC AGTTCCTTGC CAATGCTGGA
    ATGAATGGAC TGGCCGAAGG ATTCTTGGAC TTATTCTCTG TAAAGGCAGT TTACATGGGA
    CATCCGGGAA TTGATATGCA CACTGTGTGT GTTCAAAACA AACTATGTAA TATGAACCTG
    GAAGAAAATG GTGTCACATT GCTTTATGGG CTGCACACTA CTGACAACAA GTTGCTGCAC
    TTTGTAGCTC CGAAATACAC AGCCAGGACA TTGTATGATG GACTCCTGGA GTTAACCAAA
    GCAGTAAGGA AGATAAAACG CTTTCCTGAT CAGAGATTAC AGTGGTTAAG GAAGCAGTAT
    GTTAGTCTCT ACCAGGAGGA AAACAGATTT GAAGGACCAG CTTTGGCACA AGCTATTGAG
    CTGTTTGGAG GGCGACGCTG GAGTACGAGA AACACGAGCA CAGGGACCTT AACCAAAAGC
    ACAGAGAAAC CAAACGTGCA GAGGAATAAC ACTCTCGGCA TCAACACTGC CAAGAAGAAG
    AAGAAAGTGC TTATGAGGGG CGAGAGTGGG GATGCAGCCG ATGATGAGAT GGCAACCCGC
    AAAACAAAAA GCTGCAAGGA AAGTCGCAGT AGAAGTGGCT CTGATCCTCC AGAAATCGAT
    GAGCAGGAAG AGCAAGATCT GAATATTATT GCTGGATATT CACCATCTCA AATGCTCCCA
    TCCCGAAGAG CTCATTCTAT GTCGACTTCT GGTTCTCCCA ACTTGACACC CGGGCCATCT
    ATTCCACTGC GACCTGCCTC CTCTCCTATT TTGTCCAACT CAAACAAACC CCAGTCCAAT
    ACGTGGAGCA GTTCAAGTTG GCATGGAAGA GTTAAAGGTG GGATGAAAGG CTTCCAATCA
    TTCATGGTTT CGGACAGCAA CATGAACTTT ACTGAATTTG TAGAGCTTTT CAAATCTTTT
    AGCGTAAGAA GTCGGAAAGA CCTGAAGGAC ATTTTTGATG TGTATGCTGT GGCCTGCAAT
    CGGTCAGGTG CAGAATCAGT CCCTCTCTAC ACTAATTTGA CAATTGATGA GAATGTAGTT
    GGAGTCCAAC CTGATTTAGA TTTATTGACC AGGAATGTGT CCGACTTGGG TTTATTCATT
    CGCAGCAGAC AGCAACTGTC TGAGAACCAA AGGCAAATCT CAGATGCCAT TGCAGCTGCC
    AGCATTGTAA CTAATGGAAC TGGAGTAGAA AGCACATCCC TGGGCATATT TGGAATGGGA
    ATACTGCAGT TTAATGATTT CCTGGTGAAT TGCCAGGGTG AACATTACAC CTATGATGAA
    GTTCTTAGCA TTATTCAGAA ATTTGAACCA AGTATCAGTA TGCGCCAGCA GGGGCTGATG
    TCATTTGAAG GATTTGCAAG GTTCCTGATG GATAAAGACA ATTTTGCATC AAGAAATGAT
    GAGTCTCAGG TAAACACAGA AGAACTTCAG CTGCCCCTTT CCTACTATTT CATTGAGTCT
    TCACACAACA CCTACCTTAC AGGGCACCAG CTAAAAGGAG AGTCTTCAGT TGAGCTGTAC
    AGCCAGGTCC TTTTGCAGGG GTGCCGAAGT GTTGAATTAG ACTGCTGGGA TGGAGATGAC
    GGCATGCCTA TCATTTATCA TGGACATACA CTAACTACTA AAATTCCCTT TAAGGATGTC
    ATTGAAGCTA TTGGCCGAAG TGCATTTATT ACCTCTGAAA TGCCAATCGT ACTGTCCATA
    GAAAACCATT GCTCCCTACC ACAGCAGCGT AAAATGGCAG ATATATTTAA GAATGTCTTT
    GGTGAAAAAC TGGTGACAAA GTTCCTGTTT GAAAGTGACT TTTCAGATGA TCCGATGTTG
    CCTTCTCCAT GGCAATTGAG AAATAAAGTG CTTCTGAAAA ATAAAAAGCT GAAGGCACAT
    CAAACCCCGG TGGATATACT GAAGCAGAAG GCACATCAGC TGGCTAGCAT GCAAGCACAA
    GCAAGCAATG GTAGCCAAAT GGTAAGCCCT AGCACAAACA ATGAAGAAGA AGAAGATGAA
    GAAGATGAAT ATGATTATGA CTATGAGTCT CTGTCAGATG CCGATGTCCT TAATGCTTCC
    CCTGCTCCTA ACAGCCAGGA AGATAATATT CTTGATGACC GATCTGAAAC TAAAACCAAC
    AGCGACAAAC TGCAATTTGA ATATAATGAG GAAGCTTCAA AGCGAATCAA AAAGACAGAT
    GGCAGCAGTA TTAACACTAA AGGAAAGGTC TATGACATGG AGTTGGGTGA GGAATTCTAT
    CTTCCACAAA ACAAAAAAGA GAGCAGGCAA ATTGCACCTG AATTGTCAGA CCTTATTATC
    TATTGCCAAG CTGTCAAATT CCCAGGCCTT ACAACATTAA ACCCATGTGG ATCTGGCAGA
    GGGAAGGAAA GAAAAAGCAG GAAGTCCATT TTTGGCAACA ACCCTGGCAG AACAAGTCCT
    GGGGAACCAA CAGCACTTGC CAAAACATCT GGAAAAGGTA CAAATGAAAC ACGGCAGAGC
    TGGGAAGAGC CATGTTCTCC TCCCTTTAAC CCCAGCACTT CCCTCAGTGC AATAATCAGG
    ACACCCAGGT GCTACCATAT CTCTTCCCTA AATGAGAACG CAGCCAAGCG CTTGTGCCGA
    AGGTATTCTC AGAAACTCAT TCAACATACC GCATATCAAC TGCTCCGAAC ATATCCCGCC
    GCCACTCGGA TAGACTCTTC CAACCCACAT CCTCTTATTT TCTGGCTTCA TGGTGTACAG
    TTGGTTGCTC TAAACTACCA AACTGATGAT CTCCCCTTAC ATCTAAACGC CGCCATGTTT
    GAAGCAAATG GTGGTTGCGG ATATGTGTTA AAGCCCCCCG TGCAGTGGGA TAGGACCTGC
    CCAATGTACC AATTATTCTC ACCATTAGAA CGAGATTTAG AAAATATGGA ACCAGCAATA
    TATTCTTTGA CGATAGTCTC TGGGCAAAAT GTGTGTCCAG GTAACAGCTC AGGCAGCCCC
    TGCATTGAAA TTGATGTCTT GGGTATGCCA GTAGACAGCT GCCATTTTCG GACCAAACCA
    ATCCATCGCA ATACCTTAAA TCCCATGTGG AATGAACAAT TCTTATTCCG AATATACTTT
    GAGGATCTTG TGTTTCTTCG GTTTGCTGTG GTGGAAAACA ACAGTTCTGC TGTAACTGCC
    CAAAGAATAA TTAATCTTAA AGCTTTAAAG AGAGGTAACA GACACCTTCA GCTAAGAAAT
    TTACATAATG AGCCCTTAGA GGTTTCTACC TTATTTATAA ATAGCAGGAG AATGGAAGAA
    ATCCCTAATG GAAATACCTT ACCCGCCTCC TTGTTTTTCA GCTCAGAAGA AAGAAAGACT
    CCAGCCACAT TTAAAGCTAC AGTACATGGG ATTCCTGGGC CAGAACCATT TACAGTGTTT
    GAAATCAGTC TTGGGACAAC TGCAAAGCAA CTTCTTGATC ATATTTTGGC TACTATACAA
    GGCAATGAAG CAGACATCAC TGACTATTTT TTGATGGAGG AAAAATGCTT TATATCTAAA
    GACAAAAATG AATGCAAGAA ATTGCCGTTC CAGAGAGTGA TTAGCCCAGA AGAGGATATC
    CTGCAAATTT TGAATAGCTG GTTCCCAGAA GAAGGCTACG TGGGGAGAAT TGTTCTTAAA
    ACACGAGAGG AGAACATGAA TGACAAAAAT GTTCAAGAGG ACAAAGAAGA AAAAGAGGTG
    ACCGTGAGCT CCGATGAGGA GACTTTCTTT GTCCAGGTCC ATGATGTTTC TCCTGAACAG
    CCAAGAACGG TTATCAAAGC TTTACGATTC AGTACAGCGC AGGAAGTTAT CCAGCAGGCT
    TTATGCAAGG CTAAATACTC ATACAGCATC CTGAGCAACC CTAATCCTAG TGATTATGTG
    CTTCTGGAGG AAGTCATCAA AGAAGCTTCA AATAAGAAGT CTTCTACCTC CAAAACAATT
    CAGAGGGTTC TCTTGGATCA AGAGTGTGTA TTCCAGGCAC AGAGCAAGTG GAAGGGAGCA
    GGGAAATTCA TTCTTAAACT GAAGGAACAA GTCCAGGCGG CAAGAGACGA CAAGCGCAAA
    GGTCTGTCTA TAGCCAGTGA ACTGAAGAAG CTAGCAAAGT CCAGCCGGCA ATCTCGGAAC
    TTCACTTTGT CACCTCAGAT ATTTTTATCA GAAGATGCCT GTGAAAGCCC TGAACCTACA
    AAAAGCAAAA CGCAGGAAAA GCCTTCGGCA GATAATGAAG TAAATGTGCA TCCAACCTGC
    TGCAGGAGGT CTTCTGCTGC CTGGTACTTT CTGTTGCTGA AGAACTGGAA GCTGCCAAAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62378
    Clone ID Related Accession (Same CDS sequence) XM_031905944.1 , XM_031905943.1
    Accession Version XM_031905944.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCACTC CAAGAAGGTC
    TATGAGCCAA ACCGGGTTCT GTTTATTATG GGAGATTTAT CTGACTCTGA AAGTGATATC
    TTTGAACAAT CAAAAGAGTG GGACTTGCAT AGGAGGGAAG AGCAACAGAA GGCATTCAGC
    CATGGAACAG AGCTAATTCC CTGGTATGTG TTATCCATGA GAGCAGATGT CTATCAGTTT
    CTTCAGCAAG GAGTGACTGT CATCCGCTAT GACCAGGAGA CACACATTTC TGTTCGTTGT
    TTCCTTCAAC TCCAACCAGA CAACTCCACA TTGACTTGGA CAAAACCAAC CACAACGTGT
    CTTGCAAACA CTAAAAATAA ATTAGGAACT GTAAGTAGCA GTGCGGAGAT TAAATTTCAG
    TTCCTTGCCA ATGCTGGAAT GAATGGACTG GCCGAAGGAT TCTTGGACTT ATTCTCTGTA
    AAGGCAGTTT ACATGGGACA TCCGGGAATT GATATGCACA CTGTGTGTGT TCAAAACAAA
    CTATGTAATA TGAACCTGGA AGAAAATGGT GTCACATTGC TTTATGGGCT GCACACTACT
    GACAACAAGT TGCTGCACTT TGTAGCTCCG AAATACACAG CCAGGACATT GTATGATGGA
    CTCCTGGAGT TAACCAAAGC AGTAAGGAAG ATAAAACGCT TTCCTGATCA GAGATTACAG
    TGGTTAAGGA AGCAGTATGT TAGTCTCTAC CAGGAGGAAA ACAGATTTGA AGGACCAGCT
    TTGGCACAAG CTATTGAGCT GTTTGGAGGG CGACGCTGGA GTACGAGAAA CACGAGCACA
    GGGACCTTAA CCAAAAGCAC AGAGAAACCA AACGTGCAGA GGAATAACAC TCTCGGCATC
    AACACTGCCA AGAAGAAGAA GAAAGTGCTT ATGAGGGGCG AGAGTGGGGA TGCAGCCGAT
    GATGAGATGG CAACCCGCAA AACAAAAAGC TGCAAGGAAA GTCGCAGTAG AAGTGGCTCT
    GATCCTCCAG AAATCGATGA GCAGGAAGAG CAAGATCTGA ATATTATTGC TGGATATTCA
    CCATCTCAAA TGCTCCCATC CCGAAGAGCT CATTCTATGT CGACTTCTGG TTCTCCCAAC
    TTGACACCCG GGCCATCTAT TCCACTGCGA CCTGCCTCCT CTCCTATTTT GTCCAACTCA
    AACAAACCCC AGTCCAATAC GTGGAGCAGT TCAAGTTGGC ATGGAAGAGT TAAAGGTGGG
    ATGAAAGGCT TCCAATCATT CATGGTTTCG GACAGCAACA TGAACTTTAC TGAATTTGTA
    GAGCTTTTCA AATCTTTTAG CGTAAGAAGT CGGAAAGACC TGAAGGACAT TTTTGATGTG
    TATGCTGTGG CCTGCAATCG GTCAGGTGCA GAATCAGTCC CTCTCTACAC TAATTTGACA
    ATTGATGAGA ATGTAGTTGG AGTCCAACCT GATTTAGATT TATTGACCAG GAATGTGTCC
    GACTTGGGTT TATTCATTCG CAGCAGACAG CAACTGTCTG AGAACCAAAG GCAAATCTCA
    GATGCCATTG CGGCTGCCAG CATTGTAACT AATGGAACTG GAGTAGAAAG CACATCCCTG
    GGCATATTTG GAATGGGAAT ACTGCAGTTT AATGATTTCC TGGTGAATTG CCAGGGTGAA
    CATTACACCT ATGATGAAGT TCTTAGCATT ATTCAGAAAT TTGAACCAAG TATCAGTATG
    CGCCAGCAGG GGCTGATGTC ATTTGAAGGA TTTGCAAGGT TCCTGATGGA TAAAGACAAT
    TTTGCATCAA GAAATGATGA GTCTCAGGTA AACACAGAAG AACTTCAGCT GCCCCTTTCC
    TACTATTTCA TTGAGTCTTC ACACAACACC TACCTTACAG GGCACCAGCT AAAAGGAGAG
    TCTTCAGTTG AGCTGTACAG CCAGGTCCTT TTGCAGGGGT GCCGAAGTGT TGAATTAGAC
    TGCTGGGATG GAGATGACGG CATGCCTATC ATTTATCATG GACATACACT AACTACTAAA
    ATTCCCTTTA AGGATGTCAT TGAAGCTATT GGCCGAAGTG CATTTATTAC CTCTGAAATG
    CCAATCGTAC TGTCCATAGA AAACCATTGC TCCCTACCAC AGCAGCGTAA AATGGCAGAT
    ATATTTAAGA ATGTCTTTGG TGAAAAACTG GTGACAAAGT TCCTGTTTGA AAGTGACTTT
    TCAGATGATC CGATGTTGCC TTCTCCATGG CAATTGAGAA ATAAAGTGCT TCTGAAAAAT
    AAAAAGCTGA AGGCACATCA AACCCCGGTG GATATACTGA AGCAGAAGGC ACATCAGCTG
    GCTAGCATGC AAGCACAAGC AAGCAATGGT AGCCAAATGG TAAGCCCTAG CACAAACAAT
    GAAGAAGAAG AAGATGAAGA AGATGAATAT GATTATGACT ATGAGTCTCT GTCAGATGCC
    GATGTCCTTA ATGCTTCCCC TGCTCCTAAC AGCCAGGAAG ATAATATTCT TGATGACCGA
    TCTGAAACTA AAACCAACAG CGACAAACTG CAATTTGAAT ATAATGAGGA AGCTTCAAAG
    CGAATCAAAA AGACAGATGG CAGCAGTATT AACACTAAAG GAAAGGTCTA TGACATGGAG
    TTGGGTGAGG AATTCTATCT TCCACAAAAC AAAAAAGAGA GCAGGCAAAT TGCACCTGAA
    TTGTCAGACC TTATTATCTA TTGCCAAGCT GTCAAATTCC CAGGCCTTAC AACATTAAAC
    CCATGTGGAT CTGGCAGAGG GAAGGAAAGA AAAAGCAGGA AGTCCATTTT TGGCAACAAC
    CCTGGCAGAA CAAGTCCTGG GGAACCAACA GCACTTGCCA AAACATCTGG AAAAGGTACA
    AATGAAACAC GGCAGAGCTG GGAAGAGCCA TGTTCTCCTC CCTTTAACCC CAGCACTTCC
    CTCAGTGCAA TAATCAGGAC ACCCAGGTGC TACCATATCT CTTCCCTAAA TGAGAACGCA
    GCCAAGCGCT TGTGCCGAAG GTATTCTCAG AAACTCATTC AACATACCGC ATATCAACTG
    CTCCGAACAT ATCCCGCCGC CACTCGGATA GACTCTTCCA ACCCACATCC TCTTATTTTC
    TGGCTTCATG GTGTACAGTT GGTTGCTCTA AACTACCAAA CTGATGATCT CCCCTTACAT
    CTAAACGCCG CCATGTTTGA AGCAAATGGT GGTTGCGGAT ATGTGTTAAA GCCCCCCGTG
    CAGTGGGATA GGACCTGCCC AATGTACCAA TTATTCTCAC CATTAGAACG AGATTTAGAA
    AATATGGAAC CAGCAATATA TTCTTTGACG ATAGTCTCTG GGCAAAATGT GTGTCCAGGT
    AACAGCTCAG GCAGCCCCTG CATTGAAATT GATGTCTTGG GTATGCCAGT AGACAGCTGC
    CATTTTCGGA CCAAACCAAT CCATCGCAAT ACCTTAAATC CCATGTGGAA TGAACAATTC
    TTATTCCGAA TATACTTTGA GGATCTTGTG TTTCTTCGGT TTGCTGTGGT GGAAAACAAC
    AGCTCTGCTG TAACTGCCCA AAGAATAATT AATCTTAAAG CTTTAAAGAG AGGTAACAGA
    CACCTTCAGC TAAGAAATTT ACATAATGAG CCCTTAGAGG TTTCTACCTT ATTTATAAAT
    AGCAGGAGAA TGGAAGAAAT CCCTAATGGA AATACCTTAC CCGCCTCCTT GTTTTTCAGC
    TCAGAAGAAA GAAAGACTCC AGCCACATTT AAAGCTACAG TACATGGGAT TCCTGGGCCA
    GAACCATTTA CAGTGTTTGA AATCAGTCTT GGGACAACTG CAAAGCAACT TCTTGATCAT
    ATTTTGGCTA CTATACAAGG CAATGAAGCA GACATCACTG ACTATTTTTT GATGGAGGAA
    AAATGCTTTA TATCTAAAGA CAAAAATGAA TGCAAGAAAT TGCCGTTCCA GAGAGTGATT
    AGCCCAGAAG AGGATATCCT GCAAATTTTG AATAGCTGGT TCCCAGAAGA AGGCTACGTG
    GGGAGAATTG TTCTTAAAAC ACGAGAGGAG AACATGAATG ACAAAAATGT TCAAGAGGAC
    AAAGAAGAAA AAGAGGTGAC CGTGAGCTCC GATGAGGAGA CTTTCTTTGT CCAGGTCCAT
    GATGTTTCTC CTGAACAGCC AAGAACGGTT ATCAAAGCTT TACGATTCAG TACAGCGCAG
    GAAGTTATCC AGCAGGCTTT ATGCAAGGCT AAATACTCAT ACAGCATCCT GAGCAACCCT
    AATCCTAGTG ATTATGTGCT TCTGGAGGAA GTCATCAAAG AAGCTTCAAA TAAGAAGTCT
    TCTACCTCCA AAACAATTCA GAGGGTTCTC TTGGATCAAG AGTGTGTATT CCAGGCACAG
    AGCAAGTGGA AGGGAGCAGG GAAATTCATT CTTAAACTGA AGGAACAAGT CCAGGCGGCA
    AGAGACGACA AGCGCAAAGG TCTGTCTATA GCCAGTGAAC TGAAGAAGCT AGCAAAGTCC
    AGCCGGCAAT CTCGGAACTT CACTTTGTCA CCTCAGATAT TTTTATCAGA AGATGCCTGT
    GAAAGCCCTG AACCTACAAA AAGCAAAACG CAGGAAAAGC CTTCGGCAGA TAATGAAGTA
    AATGTGCATC CAACCTGCTG CAGGAGGTCT TCTGCTGCCT GGTACTTTCT GTTGCTGAAG
    AACTGGAAGC TGCCAAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761804.1
    CDS547..7587
    Translation

    Target ORF information:

    RefSeq Version XM_031905944.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905944.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCACTC CAAGAAGGTC
    TATGAGCCAA ACCGGGTTCT GTTTATTATG GGAGATTTAT CTGACTCTGA AAGTGATATC
    TTTGAACAAT CAAAAGAGTG GGACTTGCAT AGGAGGGAAG AGCAACAGAA GGCATTCAGC
    CATGGAACAG AGCTAATTCC CTGGTATGTG TTATCCATGA GAGCAGATGT CTATCAGTTT
    CTTCAGCAAG GAGTGACTGT CATCCGCTAT GACCAGGAGA CACACATTTC TGTTCGTTGT
    TTCCTTCAAC TCCAACCAGA CAACTCCACA TTGACTTGGA CAAAACCAAC CACAACGTGT
    CTTGCAAACA CTAAAAATAA ATTAGGAACT GTAAGTAGCA GTGCGGAGAT TAAATTTCAG
    TTCCTTGCCA ATGCTGGAAT GAATGGACTG GCCGAAGGAT TCTTGGACTT ATTCTCTGTA
    AAGGCAGTTT ACATGGGACA TCCGGGAATT GATATGCACA CTGTGTGTGT TCAAAACAAA
    CTATGTAATA TGAACCTGGA AGAAAATGGT GTCACATTGC TTTATGGGCT GCACACTACT
    GACAACAAGT TGCTGCACTT TGTAGCTCCG AAATACACAG CCAGGACATT GTATGATGGA
    CTCCTGGAGT TAACCAAAGC AGTAAGGAAG ATAAAACGCT TTCCTGATCA GAGATTACAG
    TGGTTAAGGA AGCAGTATGT TAGTCTCTAC CAGGAGGAAA ACAGATTTGA AGGACCAGCT
    TTGGCACAAG CTATTGAGCT GTTTGGAGGG CGACGCTGGA GTACGAGAAA CACGAGCACA
    GGGACCTTAA CCAAAAGCAC AGAGAAACCA AACGTGCAGA GGAATAACAC TCTCGGCATC
    AACACTGCCA AGAAGAAGAA GAAAGTGCTT ATGAGGGGCG AGAGTGGGGA TGCAGCCGAT
    GATGAGATGG CAACCCGCAA AACAAAAAGC TGCAAGGAAA GTCGCAGTAG AAGTGGCTCT
    GATCCTCCAG AAATCGATGA GCAGGAAGAG CAAGATCTGA ATATTATTGC TGGATATTCA
    CCATCTCAAA TGCTCCCATC CCGAAGAGCT CATTCTATGT CGACTTCTGG TTCTCCCAAC
    TTGACACCCG GGCCATCTAT TCCACTGCGA CCTGCCTCCT CTCCTATTTT GTCCAACTCA
    AACAAACCCC AGTCCAATAC GTGGAGCAGT TCAAGTTGGC ATGGAAGAGT TAAAGGTGGG
    ATGAAAGGCT TCCAATCATT CATGGTTTCG GACAGCAACA TGAACTTTAC TGAATTTGTA
    GAGCTTTTCA AATCTTTTAG CGTAAGAAGT CGGAAAGACC TGAAGGACAT TTTTGATGTG
    TATGCTGTGG CCTGCAATCG GTCAGGTGCA GAATCAGTCC CTCTCTACAC TAATTTGACA
    ATTGATGAGA ATGTAGTTGG AGTCCAACCT GATTTAGATT TATTGACCAG GAATGTGTCC
    GACTTGGGTT TATTCATTCG CAGCAGACAG CAACTGTCTG AGAACCAAAG GCAAATCTCA
    GATGCCATTG CGGCTGCCAG CATTGTAACT AATGGAACTG GAGTAGAAAG CACATCCCTG
    GGCATATTTG GAATGGGAAT ACTGCAGTTT AATGATTTCC TGGTGAATTG CCAGGGTGAA
    CATTACACCT ATGATGAAGT TCTTAGCATT ATTCAGAAAT TTGAACCAAG TATCAGTATG
    CGCCAGCAGG GGCTGATGTC ATTTGAAGGA TTTGCAAGGT TCCTGATGGA TAAAGACAAT
    TTTGCATCAA GAAATGATGA GTCTCAGGTA AACACAGAAG AACTTCAGCT GCCCCTTTCC
    TACTATTTCA TTGAGTCTTC ACACAACACC TACCTTACAG GGCACCAGCT AAAAGGAGAG
    TCTTCAGTTG AGCTGTACAG CCAGGTCCTT TTGCAGGGGT GCCGAAGTGT TGAATTAGAC
    TGCTGGGATG GAGATGACGG CATGCCTATC ATTTATCATG GACATACACT AACTACTAAA
    ATTCCCTTTA AGGATGTCAT TGAAGCTATT GGCCGAAGTG CATTTATTAC CTCTGAAATG
    CCAATCGTAC TGTCCATAGA AAACCATTGC TCCCTACCAC AGCAGCGTAA AATGGCAGAT
    ATATTTAAGA ATGTCTTTGG TGAAAAACTG GTGACAAAGT TCCTGTTTGA AAGTGACTTT
    TCAGATGATC CGATGTTGCC TTCTCCATGG CAATTGAGAA ATAAAGTGCT TCTGAAAAAT
    AAAAAGCTGA AGGCACATCA AACCCCGGTG GATATACTGA AGCAGAAGGC ACATCAGCTG
    GCTAGCATGC AAGCACAAGC AAGCAATGGT AGCCAAATGG TAAGCCCTAG CACAAACAAT
    GAAGAAGAAG AAGATGAAGA AGATGAATAT GATTATGACT ATGAGTCTCT GTCAGATGCC
    GATGTCCTTA ATGCTTCCCC TGCTCCTAAC AGCCAGGAAG ATAATATTCT TGATGACCGA
    TCTGAAACTA AAACCAACAG CGACAAACTG CAATTTGAAT ATAATGAGGA AGCTTCAAAG
    CGAATCAAAA AGACAGATGG CAGCAGTATT AACACTAAAG GAAAGGTCTA TGACATGGAG
    TTGGGTGAGG AATTCTATCT TCCACAAAAC AAAAAAGAGA GCAGGCAAAT TGCACCTGAA
    TTGTCAGACC TTATTATCTA TTGCCAAGCT GTCAAATTCC CAGGCCTTAC AACATTAAAC
    CCATGTGGAT CTGGCAGAGG GAAGGAAAGA AAAAGCAGGA AGTCCATTTT TGGCAACAAC
    CCTGGCAGAA CAAGTCCTGG GGAACCAACA GCACTTGCCA AAACATCTGG AAAAGGTACA
    AATGAAACAC GGCAGAGCTG GGAAGAGCCA TGTTCTCCTC CCTTTAACCC CAGCACTTCC
    CTCAGTGCAA TAATCAGGAC ACCCAGGTGC TACCATATCT CTTCCCTAAA TGAGAACGCA
    GCCAAGCGCT TGTGCCGAAG GTATTCTCAG AAACTCATTC AACATACCGC ATATCAACTG
    CTCCGAACAT ATCCCGCCGC CACTCGGATA GACTCTTCCA ACCCACATCC TCTTATTTTC
    TGGCTTCATG GTGTACAGTT GGTTGCTCTA AACTACCAAA CTGATGATCT CCCCTTACAT
    CTAAACGCCG CCATGTTTGA AGCAAATGGT GGTTGCGGAT ATGTGTTAAA GCCCCCCGTG
    CAGTGGGATA GGACCTGCCC AATGTACCAA TTATTCTCAC CATTAGAACG AGATTTAGAA
    AATATGGAAC CAGCAATATA TTCTTTGACG ATAGTCTCTG GGCAAAATGT GTGTCCAGGT
    AACAGCTCAG GCAGCCCCTG CATTGAAATT GATGTCTTGG GTATGCCAGT AGACAGCTGC
    CATTTTCGGA CCAAACCAAT CCATCGCAAT ACCTTAAATC CCATGTGGAA TGAACAATTC
    TTATTCCGAA TATACTTTGA GGATCTTGTG TTTCTTCGGT TTGCTGTGGT GGAAAACAAC
    AGCTCTGCTG TAACTGCCCA AAGAATAATT AATCTTAAAG CTTTAAAGAG AGGTAACAGA
    CACCTTCAGC TAAGAAATTT ACATAATGAG CCCTTAGAGG TTTCTACCTT ATTTATAAAT
    AGCAGGAGAA TGGAAGAAAT CCCTAATGGA AATACCTTAC CCGCCTCCTT GTTTTTCAGC
    TCAGAAGAAA GAAAGACTCC AGCCACATTT AAAGCTACAG TACATGGGAT TCCTGGGCCA
    GAACCATTTA CAGTGTTTGA AATCAGTCTT GGGACAACTG CAAAGCAACT TCTTGATCAT
    ATTTTGGCTA CTATACAAGG CAATGAAGCA GACATCACTG ACTATTTTTT GATGGAGGAA
    AAATGCTTTA TATCTAAAGA CAAAAATGAA TGCAAGAAAT TGCCGTTCCA GAGAGTGATT
    AGCCCAGAAG AGGATATCCT GCAAATTTTG AATAGCTGGT TCCCAGAAGA AGGCTACGTG
    GGGAGAATTG TTCTTAAAAC ACGAGAGGAG AACATGAATG ACAAAAATGT TCAAGAGGAC
    AAAGAAGAAA AAGAGGTGAC CGTGAGCTCC GATGAGGAGA CTTTCTTTGT CCAGGTCCAT
    GATGTTTCTC CTGAACAGCC AAGAACGGTT ATCAAAGCTT TACGATTCAG TACAGCGCAG
    GAAGTTATCC AGCAGGCTTT ATGCAAGGCT AAATACTCAT ACAGCATCCT GAGCAACCCT
    AATCCTAGTG ATTATGTGCT TCTGGAGGAA GTCATCAAAG AAGCTTCAAA TAAGAAGTCT
    TCTACCTCCA AAACAATTCA GAGGGTTCTC TTGGATCAAG AGTGTGTATT CCAGGCACAG
    AGCAAGTGGA AGGGAGCAGG GAAATTCATT CTTAAACTGA AGGAACAAGT CCAGGCGGCA
    AGAGACGACA AGCGCAAAGG TCTGTCTATA GCCAGTGAAC TGAAGAAGCT AGCAAAGTCC
    AGCCGGCAAT CTCGGAACTT CACTTTGTCA CCTCAGATAT TTTTATCAGA AGATGCCTGT
    GAAAGCCCTG AACCTACAAA AAGCAAAACG CAGGAAAAGC CTTCGGCAGA TAATGAAGTA
    AATGTGCATC CAACCTGCTG CAGGAGGTCT TCTGCTGCCT GGTACTTTCT GTTGCTGAAG
    AACTGGAAGC TGCCAAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62378
    Clone ID Related Accession (Same CDS sequence) XM_031905944.1 , XM_031905943.1
    Accession Version XM_031905943.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCACTC CAAGAAGGTC
    TATGAGCCAA ACCGGGTTCT GTTTATTATG GGAGATTTAT CTGACTCTGA AAGTGATATC
    TTTGAACAAT CAAAAGAGTG GGACTTGCAT AGGAGGGAAG AGCAACAGAA GGCATTCAGC
    CATGGAACAG AGCTAATTCC CTGGTATGTG TTATCCATGA GAGCAGATGT CTATCAGTTT
    CTTCAGCAAG GAGTGACTGT CATCCGCTAT GACCAGGAGA CACACATTTC TGTTCGTTGT
    TTCCTTCAAC TCCAACCAGA CAACTCCACA TTGACTTGGA CAAAACCAAC CACAACGTGT
    CTTGCAAACA CTAAAAATAA ATTAGGAACT GTAAGTAGCA GTGCGGAGAT TAAATTTCAG
    TTCCTTGCCA ATGCTGGAAT GAATGGACTG GCCGAAGGAT TCTTGGACTT ATTCTCTGTA
    AAGGCAGTTT ACATGGGACA TCCGGGAATT GATATGCACA CTGTGTGTGT TCAAAACAAA
    CTATGTAATA TGAACCTGGA AGAAAATGGT GTCACATTGC TTTATGGGCT GCACACTACT
    GACAACAAGT TGCTGCACTT TGTAGCTCCG AAATACACAG CCAGGACATT GTATGATGGA
    CTCCTGGAGT TAACCAAAGC AGTAAGGAAG ATAAAACGCT TTCCTGATCA GAGATTACAG
    TGGTTAAGGA AGCAGTATGT TAGTCTCTAC CAGGAGGAAA ACAGATTTGA AGGACCAGCT
    TTGGCACAAG CTATTGAGCT GTTTGGAGGG CGACGCTGGA GTACGAGAAA CACGAGCACA
    GGGACCTTAA CCAAAAGCAC AGAGAAACCA AACGTGCAGA GGAATAACAC TCTCGGCATC
    AACACTGCCA AGAAGAAGAA GAAAGTGCTT ATGAGGGGCG AGAGTGGGGA TGCAGCCGAT
    GATGAGATGG CAACCCGCAA AACAAAAAGC TGCAAGGAAA GTCGCAGTAG AAGTGGCTCT
    GATCCTCCAG AAATCGATGA GCAGGAAGAG CAAGATCTGA ATATTATTGC TGGATATTCA
    CCATCTCAAA TGCTCCCATC CCGAAGAGCT CATTCTATGT CGACTTCTGG TTCTCCCAAC
    TTGACACCCG GGCCATCTAT TCCACTGCGA CCTGCCTCCT CTCCTATTTT GTCCAACTCA
    AACAAACCCC AGTCCAATAC GTGGAGCAGT TCAAGTTGGC ATGGAAGAGT TAAAGGTGGG
    ATGAAAGGCT TCCAATCATT CATGGTTTCG GACAGCAACA TGAACTTTAC TGAATTTGTA
    GAGCTTTTCA AATCTTTTAG CGTAAGAAGT CGGAAAGACC TGAAGGACAT TTTTGATGTG
    TATGCTGTGG CCTGCAATCG GTCAGGTGCA GAATCAGTCC CTCTCTACAC TAATTTGACA
    ATTGATGAGA ATGTAGTTGG AGTCCAACCT GATTTAGATT TATTGACCAG GAATGTGTCC
    GACTTGGGTT TATTCATTCG CAGCAGACAG CAACTGTCTG AGAACCAAAG GCAAATCTCA
    GATGCCATTG CGGCTGCCAG CATTGTAACT AATGGAACTG GAGTAGAAAG CACATCCCTG
    GGCATATTTG GAATGGGAAT ACTGCAGTTT AATGATTTCC TGGTGAATTG CCAGGGTGAA
    CATTACACCT ATGATGAAGT TCTTAGCATT ATTCAGAAAT TTGAACCAAG TATCAGTATG
    CGCCAGCAGG GGCTGATGTC ATTTGAAGGA TTTGCAAGGT TCCTGATGGA TAAAGACAAT
    TTTGCATCAA GAAATGATGA GTCTCAGGTA AACACAGAAG AACTTCAGCT GCCCCTTTCC
    TACTATTTCA TTGAGTCTTC ACACAACACC TACCTTACAG GGCACCAGCT AAAAGGAGAG
    TCTTCAGTTG AGCTGTACAG CCAGGTCCTT TTGCAGGGGT GCCGAAGTGT TGAATTAGAC
    TGCTGGGATG GAGATGACGG CATGCCTATC ATTTATCATG GACATACACT AACTACTAAA
    ATTCCCTTTA AGGATGTCAT TGAAGCTATT GGCCGAAGTG CATTTATTAC CTCTGAAATG
    CCAATCGTAC TGTCCATAGA AAACCATTGC TCCCTACCAC AGCAGCGTAA AATGGCAGAT
    ATATTTAAGA ATGTCTTTGG TGAAAAACTG GTGACAAAGT TCCTGTTTGA AAGTGACTTT
    TCAGATGATC CGATGTTGCC TTCTCCATGG CAATTGAGAA ATAAAGTGCT TCTGAAAAAT
    AAAAAGCTGA AGGCACATCA AACCCCGGTG GATATACTGA AGCAGAAGGC ACATCAGCTG
    GCTAGCATGC AAGCACAAGC AAGCAATGGT AGCCAAATGG TAAGCCCTAG CACAAACAAT
    GAAGAAGAAG AAGATGAAGA AGATGAATAT GATTATGACT ATGAGTCTCT GTCAGATGCC
    GATGTCCTTA ATGCTTCCCC TGCTCCTAAC AGCCAGGAAG ATAATATTCT TGATGACCGA
    TCTGAAACTA AAACCAACAG CGACAAACTG CAATTTGAAT ATAATGAGGA AGCTTCAAAG
    CGAATCAAAA AGACAGATGG CAGCAGTATT AACACTAAAG GAAAGGTCTA TGACATGGAG
    TTGGGTGAGG AATTCTATCT TCCACAAAAC AAAAAAGAGA GCAGGCAAAT TGCACCTGAA
    TTGTCAGACC TTATTATCTA TTGCCAAGCT GTCAAATTCC CAGGCCTTAC AACATTAAAC
    CCATGTGGAT CTGGCAGAGG GAAGGAAAGA AAAAGCAGGA AGTCCATTTT TGGCAACAAC
    CCTGGCAGAA CAAGTCCTGG GGAACCAACA GCACTTGCCA AAACATCTGG AAAAGGTACA
    AATGAAACAC GGCAGAGCTG GGAAGAGCCA TGTTCTCCTC CCTTTAACCC CAGCACTTCC
    CTCAGTGCAA TAATCAGGAC ACCCAGGTGC TACCATATCT CTTCCCTAAA TGAGAACGCA
    GCCAAGCGCT TGTGCCGAAG GTATTCTCAG AAACTCATTC AACATACCGC ATATCAACTG
    CTCCGAACAT ATCCCGCCGC CACTCGGATA GACTCTTCCA ACCCACATCC TCTTATTTTC
    TGGCTTCATG GTGTACAGTT GGTTGCTCTA AACTACCAAA CTGATGATCT CCCCTTACAT
    CTAAACGCCG CCATGTTTGA AGCAAATGGT GGTTGCGGAT ATGTGTTAAA GCCCCCCGTG
    CAGTGGGATA GGACCTGCCC AATGTACCAA TTATTCTCAC CATTAGAACG AGATTTAGAA
    AATATGGAAC CAGCAATATA TTCTTTGACG ATAGTCTCTG GGCAAAATGT GTGTCCAGGT
    AACAGCTCAG GCAGCCCCTG CATTGAAATT GATGTCTTGG GTATGCCAGT AGACAGCTGC
    CATTTTCGGA CCAAACCAAT CCATCGCAAT ACCTTAAATC CCATGTGGAA TGAACAATTC
    TTATTCCGAA TATACTTTGA GGATCTTGTG TTTCTTCGGT TTGCTGTGGT GGAAAACAAC
    AGCTCTGCTG TAACTGCCCA AAGAATAATT AATCTTAAAG CTTTAAAGAG AGGTAACAGA
    CACCTTCAGC TAAGAAATTT ACATAATGAG CCCTTAGAGG TTTCTACCTT ATTTATAAAT
    AGCAGGAGAA TGGAAGAAAT CCCTAATGGA AATACCTTAC CCGCCTCCTT GTTTTTCAGC
    TCAGAAGAAA GAAAGACTCC AGCCACATTT AAAGCTACAG TACATGGGAT TCCTGGGCCA
    GAACCATTTA CAGTGTTTGA AATCAGTCTT GGGACAACTG CAAAGCAACT TCTTGATCAT
    ATTTTGGCTA CTATACAAGG CAATGAAGCA GACATCACTG ACTATTTTTT GATGGAGGAA
    AAATGCTTTA TATCTAAAGA CAAAAATGAA TGCAAGAAAT TGCCGTTCCA GAGAGTGATT
    AGCCCAGAAG AGGATATCCT GCAAATTTTG AATAGCTGGT TCCCAGAAGA AGGCTACGTG
    GGGAGAATTG TTCTTAAAAC ACGAGAGGAG AACATGAATG ACAAAAATGT TCAAGAGGAC
    AAAGAAGAAA AAGAGGTGAC CGTGAGCTCC GATGAGGAGA CTTTCTTTGT CCAGGTCCAT
    GATGTTTCTC CTGAACAGCC AAGAACGGTT ATCAAAGCTT TACGATTCAG TACAGCGCAG
    GAAGTTATCC AGCAGGCTTT ATGCAAGGCT AAATACTCAT ACAGCATCCT GAGCAACCCT
    AATCCTAGTG ATTATGTGCT TCTGGAGGAA GTCATCAAAG AAGCTTCAAA TAAGAAGTCT
    TCTACCTCCA AAACAATTCA GAGGGTTCTC TTGGATCAAG AGTGTGTATT CCAGGCACAG
    AGCAAGTGGA AGGGAGCAGG GAAATTCATT CTTAAACTGA AGGAACAAGT CCAGGCGGCA
    AGAGACGACA AGCGCAAAGG TCTGTCTATA GCCAGTGAAC TGAAGAAGCT AGCAAAGTCC
    AGCCGGCAAT CTCGGAACTT CACTTTGTCA CCTCAGATAT TTTTATCAGA AGATGCCTGT
    GAAAGCCCTG AACCTACAAA AAGCAAAACG CAGGAAAAGC CTTCGGCAGA TAATGAAGTA
    AATGTGCATC CAACCTGCTG CAGGAGGTCT TCTGCTGCCT GGTACTTTCT GTTGCTGAAG
    AACTGGAAGC TGCCAAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761803.1
    CDS567..7607
    Translation

    Target ORF information:

    RefSeq Version XM_031905943.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905943.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCACTC CAAGAAGGTC
    TATGAGCCAA ACCGGGTTCT GTTTATTATG GGAGATTTAT CTGACTCTGA AAGTGATATC
    TTTGAACAAT CAAAAGAGTG GGACTTGCAT AGGAGGGAAG AGCAACAGAA GGCATTCAGC
    CATGGAACAG AGCTAATTCC CTGGTATGTG TTATCCATGA GAGCAGATGT CTATCAGTTT
    CTTCAGCAAG GAGTGACTGT CATCCGCTAT GACCAGGAGA CACACATTTC TGTTCGTTGT
    TTCCTTCAAC TCCAACCAGA CAACTCCACA TTGACTTGGA CAAAACCAAC CACAACGTGT
    CTTGCAAACA CTAAAAATAA ATTAGGAACT GTAAGTAGCA GTGCGGAGAT TAAATTTCAG
    TTCCTTGCCA ATGCTGGAAT GAATGGACTG GCCGAAGGAT TCTTGGACTT ATTCTCTGTA
    AAGGCAGTTT ACATGGGACA TCCGGGAATT GATATGCACA CTGTGTGTGT TCAAAACAAA
    CTATGTAATA TGAACCTGGA AGAAAATGGT GTCACATTGC TTTATGGGCT GCACACTACT
    GACAACAAGT TGCTGCACTT TGTAGCTCCG AAATACACAG CCAGGACATT GTATGATGGA
    CTCCTGGAGT TAACCAAAGC AGTAAGGAAG ATAAAACGCT TTCCTGATCA GAGATTACAG
    TGGTTAAGGA AGCAGTATGT TAGTCTCTAC CAGGAGGAAA ACAGATTTGA AGGACCAGCT
    TTGGCACAAG CTATTGAGCT GTTTGGAGGG CGACGCTGGA GTACGAGAAA CACGAGCACA
    GGGACCTTAA CCAAAAGCAC AGAGAAACCA AACGTGCAGA GGAATAACAC TCTCGGCATC
    AACACTGCCA AGAAGAAGAA GAAAGTGCTT ATGAGGGGCG AGAGTGGGGA TGCAGCCGAT
    GATGAGATGG CAACCCGCAA AACAAAAAGC TGCAAGGAAA GTCGCAGTAG AAGTGGCTCT
    GATCCTCCAG AAATCGATGA GCAGGAAGAG CAAGATCTGA ATATTATTGC TGGATATTCA
    CCATCTCAAA TGCTCCCATC CCGAAGAGCT CATTCTATGT CGACTTCTGG TTCTCCCAAC
    TTGACACCCG GGCCATCTAT TCCACTGCGA CCTGCCTCCT CTCCTATTTT GTCCAACTCA
    AACAAACCCC AGTCCAATAC GTGGAGCAGT TCAAGTTGGC ATGGAAGAGT TAAAGGTGGG
    ATGAAAGGCT TCCAATCATT CATGGTTTCG GACAGCAACA TGAACTTTAC TGAATTTGTA
    GAGCTTTTCA AATCTTTTAG CGTAAGAAGT CGGAAAGACC TGAAGGACAT TTTTGATGTG
    TATGCTGTGG CCTGCAATCG GTCAGGTGCA GAATCAGTCC CTCTCTACAC TAATTTGACA
    ATTGATGAGA ATGTAGTTGG AGTCCAACCT GATTTAGATT TATTGACCAG GAATGTGTCC
    GACTTGGGTT TATTCATTCG CAGCAGACAG CAACTGTCTG AGAACCAAAG GCAAATCTCA
    GATGCCATTG CGGCTGCCAG CATTGTAACT AATGGAACTG GAGTAGAAAG CACATCCCTG
    GGCATATTTG GAATGGGAAT ACTGCAGTTT AATGATTTCC TGGTGAATTG CCAGGGTGAA
    CATTACACCT ATGATGAAGT TCTTAGCATT ATTCAGAAAT TTGAACCAAG TATCAGTATG
    CGCCAGCAGG GGCTGATGTC ATTTGAAGGA TTTGCAAGGT TCCTGATGGA TAAAGACAAT
    TTTGCATCAA GAAATGATGA GTCTCAGGTA AACACAGAAG AACTTCAGCT GCCCCTTTCC
    TACTATTTCA TTGAGTCTTC ACACAACACC TACCTTACAG GGCACCAGCT AAAAGGAGAG
    TCTTCAGTTG AGCTGTACAG CCAGGTCCTT TTGCAGGGGT GCCGAAGTGT TGAATTAGAC
    TGCTGGGATG GAGATGACGG CATGCCTATC ATTTATCATG GACATACACT AACTACTAAA
    ATTCCCTTTA AGGATGTCAT TGAAGCTATT GGCCGAAGTG CATTTATTAC CTCTGAAATG
    CCAATCGTAC TGTCCATAGA AAACCATTGC TCCCTACCAC AGCAGCGTAA AATGGCAGAT
    ATATTTAAGA ATGTCTTTGG TGAAAAACTG GTGACAAAGT TCCTGTTTGA AAGTGACTTT
    TCAGATGATC CGATGTTGCC TTCTCCATGG CAATTGAGAA ATAAAGTGCT TCTGAAAAAT
    AAAAAGCTGA AGGCACATCA AACCCCGGTG GATATACTGA AGCAGAAGGC ACATCAGCTG
    GCTAGCATGC AAGCACAAGC AAGCAATGGT AGCCAAATGG TAAGCCCTAG CACAAACAAT
    GAAGAAGAAG AAGATGAAGA AGATGAATAT GATTATGACT ATGAGTCTCT GTCAGATGCC
    GATGTCCTTA ATGCTTCCCC TGCTCCTAAC AGCCAGGAAG ATAATATTCT TGATGACCGA
    TCTGAAACTA AAACCAACAG CGACAAACTG CAATTTGAAT ATAATGAGGA AGCTTCAAAG
    CGAATCAAAA AGACAGATGG CAGCAGTATT AACACTAAAG GAAAGGTCTA TGACATGGAG
    TTGGGTGAGG AATTCTATCT TCCACAAAAC AAAAAAGAGA GCAGGCAAAT TGCACCTGAA
    TTGTCAGACC TTATTATCTA TTGCCAAGCT GTCAAATTCC CAGGCCTTAC AACATTAAAC
    CCATGTGGAT CTGGCAGAGG GAAGGAAAGA AAAAGCAGGA AGTCCATTTT TGGCAACAAC
    CCTGGCAGAA CAAGTCCTGG GGAACCAACA GCACTTGCCA AAACATCTGG AAAAGGTACA
    AATGAAACAC GGCAGAGCTG GGAAGAGCCA TGTTCTCCTC CCTTTAACCC CAGCACTTCC
    CTCAGTGCAA TAATCAGGAC ACCCAGGTGC TACCATATCT CTTCCCTAAA TGAGAACGCA
    GCCAAGCGCT TGTGCCGAAG GTATTCTCAG AAACTCATTC AACATACCGC ATATCAACTG
    CTCCGAACAT ATCCCGCCGC CACTCGGATA GACTCTTCCA ACCCACATCC TCTTATTTTC
    TGGCTTCATG GTGTACAGTT GGTTGCTCTA AACTACCAAA CTGATGATCT CCCCTTACAT
    CTAAACGCCG CCATGTTTGA AGCAAATGGT GGTTGCGGAT ATGTGTTAAA GCCCCCCGTG
    CAGTGGGATA GGACCTGCCC AATGTACCAA TTATTCTCAC CATTAGAACG AGATTTAGAA
    AATATGGAAC CAGCAATATA TTCTTTGACG ATAGTCTCTG GGCAAAATGT GTGTCCAGGT
    AACAGCTCAG GCAGCCCCTG CATTGAAATT GATGTCTTGG GTATGCCAGT AGACAGCTGC
    CATTTTCGGA CCAAACCAAT CCATCGCAAT ACCTTAAATC CCATGTGGAA TGAACAATTC
    TTATTCCGAA TATACTTTGA GGATCTTGTG TTTCTTCGGT TTGCTGTGGT GGAAAACAAC
    AGCTCTGCTG TAACTGCCCA AAGAATAATT AATCTTAAAG CTTTAAAGAG AGGTAACAGA
    CACCTTCAGC TAAGAAATTT ACATAATGAG CCCTTAGAGG TTTCTACCTT ATTTATAAAT
    AGCAGGAGAA TGGAAGAAAT CCCTAATGGA AATACCTTAC CCGCCTCCTT GTTTTTCAGC
    TCAGAAGAAA GAAAGACTCC AGCCACATTT AAAGCTACAG TACATGGGAT TCCTGGGCCA
    GAACCATTTA CAGTGTTTGA AATCAGTCTT GGGACAACTG CAAAGCAACT TCTTGATCAT
    ATTTTGGCTA CTATACAAGG CAATGAAGCA GACATCACTG ACTATTTTTT GATGGAGGAA
    AAATGCTTTA TATCTAAAGA CAAAAATGAA TGCAAGAAAT TGCCGTTCCA GAGAGTGATT
    AGCCCAGAAG AGGATATCCT GCAAATTTTG AATAGCTGGT TCCCAGAAGA AGGCTACGTG
    GGGAGAATTG TTCTTAAAAC ACGAGAGGAG AACATGAATG ACAAAAATGT TCAAGAGGAC
    AAAGAAGAAA AAGAGGTGAC CGTGAGCTCC GATGAGGAGA CTTTCTTTGT CCAGGTCCAT
    GATGTTTCTC CTGAACAGCC AAGAACGGTT ATCAAAGCTT TACGATTCAG TACAGCGCAG
    GAAGTTATCC AGCAGGCTTT ATGCAAGGCT AAATACTCAT ACAGCATCCT GAGCAACCCT
    AATCCTAGTG ATTATGTGCT TCTGGAGGAA GTCATCAAAG AAGCTTCAAA TAAGAAGTCT
    TCTACCTCCA AAACAATTCA GAGGGTTCTC TTGGATCAAG AGTGTGTATT CCAGGCACAG
    AGCAAGTGGA AGGGAGCAGG GAAATTCATT CTTAAACTGA AGGAACAAGT CCAGGCGGCA
    AGAGACGACA AGCGCAAAGG TCTGTCTATA GCCAGTGAAC TGAAGAAGCT AGCAAAGTCC
    AGCCGGCAAT CTCGGAACTT CACTTTGTCA CCTCAGATAT TTTTATCAGA AGATGCCTGT
    GAAAGCCCTG AACCTACAAA AAGCAAAACG CAGGAAAAGC CTTCGGCAGA TAATGAAGTA
    AATGTGCATC CAACCTGCTG CAGGAGGTCT TCTGCTGCCT GGTACTTTCT GTTGCTGAAG
    AACTGGAAGC TGCCAAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62379
    Clone ID Related Accession (Same CDS sequence) XM_031905945.1
    Accession Version XM_031905945.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCAGTT TATTATGGGA
    GATTTATCTG ACTCTGAAAG TGATATCTTT GAACAATCAA AAGAGTGGGA CTTGCATAGG
    AGGGAAGAGC AACAGAAGGC ATTCAGCCAT GGAACAGAGC TAATTCCCTG GTATGTGTTA
    TCCATGAGAG CAGATGTCTA TCAGTTTCTT CAGCAAGGAG TGACTGTCAT CCGCTATGAC
    CAGGAGACAC ACATTTCTGT TCGTTGTTTC CTTCAACTCC AACCAGACAA CTCCACATTG
    ACTTGGACAA AACCAACCAC AACGTGTCTT GCAAACACTA AAAATAAATT AGGAACTGTA
    AGTAGCAGTG CGGAGATTAA ATTTCAGTTC CTTGCCAATG CTGGAATGAA TGGACTGGCC
    GAAGGATTCT TGGACTTATT CTCTGTAAAG GCAGTTTACA TGGGACATCC GGGAATTGAT
    ATGCACACTG TGTGTGTTCA AAACAAACTA TGTAATATGA ACCTGGAAGA AAATGGTGTC
    ACATTGCTTT ATGGGCTGCA CACTACTGAC AACAAGTTGC TGCACTTTGT AGCTCCGAAA
    TACACAGCCA GGACATTGTA TGATGGACTC CTGGAGTTAA CCAAAGCAGT AAGGAAGATA
    AAACGCTTTC CTGATCAGAG ATTACAGTGG TTAAGGAAGC AGTATGTTAG TCTCTACCAG
    GAGGAAAACA GATTTGAAGG ACCAGCTTTG GCACAAGCTA TTGAGCTGTT TGGAGGGCGA
    CGCTGGAGTA CGAGAAACAC GAGCACAGGG ACCTTAACCA AAAGCACAGA GAAACCAAAC
    GTGCAGAGGA ATAACACTCT CGGCATCAAC ACTGCCAAGA AGAAGAAGAA AGTGCTTATG
    AGGGGCGAGA GTGGGGATGC AGCCGATGAT GAGATGGCAA CCCGCAAAAC AAAAAGCTGC
    AAGGAAAGTC GCAGTAGAAG TGGCTCTGAT CCTCCAGAAA TCGATGAGCA GGAAGAGCAA
    GATCTGAATA TTATTGCTGG ATATTCACCA TCTCAAATGC TCCCATCCCG AAGAGCTCAT
    TCTATGTCGA CTTCTGGTTC TCCCAACTTG ACACCCGGGC CATCTATTCC ACTGCGACCT
    GCCTCCTCTC CTATTTTGTC CAACTCAAAC AAACCCCAGT CCAATACGTG GAGCAGTTCA
    AGTTGGCATG GAAGAGTTAA AGGTGGGATG AAAGGCTTCC AATCATTCAT GGTTTCGGAC
    AGCAACATGA ACTTTACTGA ATTTGTAGAG CTTTTCAAAT CTTTTAGCGT AAGAAGTCGG
    AAAGACCTGA AGGACATTTT TGATGTGTAT GCTGTGGCCT GCAATCGGTC AGGTGCAGAA
    TCAGTCCCTC TCTACACTAA TTTGACAATT GATGAGAATG TAGTTGGAGT CCAACCTGAT
    TTAGATTTAT TGACCAGGAA TGTGTCCGAC TTGGGTTTAT TCATTCGCAG CAGACAGCAA
    CTGTCTGAGA ACCAAAGGCA AATCTCAGAT GCCATTGCGG CTGCCAGCAT TGTAACTAAT
    GGAACTGGAG TAGAAAGCAC ATCCCTGGGC ATATTTGGAA TGGGAATACT GCAGTTTAAT
    GATTTCCTGG TGAATTGCCA GGGTGAACAT TACACCTATG ATGAAGTTCT TAGCATTATT
    CAGAAATTTG AACCAAGTAT CAGTATGCGC CAGCAGGGGC TGATGTCATT TGAAGGATTT
    GCAAGGTTCC TGATGGATAA AGACAATTTT GCATCAAGAA ATGATGAGTC TCAGGTAAAC
    ACAGAAGAAC TTCAGCTGCC CCTTTCCTAC TATTTCATTG AGTCTTCACA CAACACCTAC
    CTTACAGGGC ACCAGCTAAA AGGAGAGTCT TCAGTTGAGC TGTACAGCCA GGTCCTTTTG
    CAGGGGTGCC GAAGTGTTGA ATTAGACTGC TGGGATGGAG ATGACGGCAT GCCTATCATT
    TATCATGGAC ATACACTAAC TACTAAAATT CCCTTTAAGG ATGTCATTGA AGCTATTGGC
    CGAAGTGCAT TTATTACCTC TGAAATGCCA ATCGTACTGT CCATAGAAAA CCATTGCTCC
    CTACCACAGC AGCGTAAAAT GGCAGATATA TTTAAGAATG TCTTTGGTGA AAAACTGGTG
    ACAAAGTTCC TGTTTGAAAG TGACTTTTCA GATGATCCGA TGTTGCCTTC TCCATGGCAA
    TTGAGAAATA AAGTGCTTCT GAAAAATAAA AAGCTGAAGG CACATCAAAC CCCGGTGGAT
    ATACTGAAGC AGAAGGCACA TCAGCTGGCT AGCATGCAAG CACAAGCAAG CAATGGTAGC
    CAAATGGTAA GCCCTAGCAC AAACAATGAA GAAGAAGAAG ATGAAGAAGA TGAATATGAT
    TATGACTATG AGTCTCTGTC AGATGCCGAT GTCCTTAATG CTTCCCCTGC TCCTAACAGC
    CAGGAAGATA ATATTCTTGA TGACCGATCT GAAACTAAAA CCAACAGCGA CAAACTGCAA
    TTTGAATATA ATGAGGAAGC TTCAAAGCGA ATCAAAAAGA CAGATGGCAG CAGTATTAAC
    ACTAAAGGAA AGGTCTATGA CATGGAGTTG GGTGAGGAAT TCTATCTTCC ACAAAACAAA
    AAAGAGAGCA GGCAAATTGC ACCTGAATTG TCAGACCTTA TTATCTATTG CCAAGCTGTC
    AAATTCCCAG GCCTTACAAC ATTAAACCCA TGTGGATCTG GCAGAGGGAA GGAAAGAAAA
    AGCAGGAAGT CCATTTTTGG CAACAACCCT GGCAGAACAA GTCCTGGGGA ACCAACAGCA
    CTTGCCAAAA CATCTGGAAA AGGTACAAAT GAAACACGGC AGAGCTGGGA AGAGCCATGT
    TCTCCTCCCT TTAACCCCAG CACTTCCCTC AGTGCAATAA TCAGGACACC CAGGTGCTAC
    CATATCTCTT CCCTAAATGA GAACGCAGCC AAGCGCTTGT GCCGAAGGTA TTCTCAGAAA
    CTCATTCAAC ATACCGCATA TCAACTGCTC CGAACATATC CCGCCGCCAC TCGGATAGAC
    TCTTCCAACC CACATCCTCT TATTTTCTGG CTTCATGGTG TACAGTTGGT TGCTCTAAAC
    TACCAAACTG ATGATCTCCC CTTACATCTA AACGCCGCCA TGTTTGAAGC AAATGGTGGT
    TGCGGATATG TGTTAAAGCC CCCCGTGCAG TGGGATAGGA CCTGCCCAAT GTACCAATTA
    TTCTCACCAT TAGAACGAGA TTTAGAAAAT ATGGAACCAG CAATATATTC TTTGACGATA
    GTCTCTGGGC AAAATGTGTG TCCAGGTAAC AGCTCAGGCA GCCCCTGCAT TGAAATTGAT
    GTCTTGGGTA TGCCAGTAGA CAGCTGCCAT TTTCGGACCA AACCAATCCA TCGCAATACC
    TTAAATCCCA TGTGGAATGA ACAATTCTTA TTCCGAATAT ACTTTGAGGA TCTTGTGTTT
    CTTCGGTTTG CTGTGGTGGA AAACAACAGC TCTGCTGTAA CTGCCCAAAG AATAATTAAT
    CTTAAAGCTT TAAAGAGAGG TAACAGACAC CTTCAGCTAA GAAATTTACA TAATGAGCCC
    TTAGAGGTTT CTACCTTATT TATAAATAGC AGGAGAATGG AAGAAATCCC TAATGGAAAT
    ACCTTACCCG CCTCCTTGTT TTTCAGCTCA GAAGAAAGAA AGACTCCAGC CACATTTAAA
    GCTACAGTAC ATGGGATTCC TGGGCCAGAA CCATTTACAG TGTTTGAAAT CAGTCTTGGG
    ACAACTGCAA AGCAACTTCT TGATCATATT TTGGCTACTA TACAAGGCAA TGAAGCAGAC
    ATCACTGACT ATTTTTTGAT GGAGGAAAAA TGCTTTATAT CTAAAGACAA AAATGAATGC
    AAGAAATTGC CGTTCCAGAG AGTGATTAGC CCAGAAGAGG ATATCCTGCA AATTTTGAAT
    AGCTGGTTCC CAGAAGAAGG CTACGTGGGG AGAATTGTTC TTAAAACACG AGAGGAGAAC
    ATGAATGACA AAAATGTTCA AGAGGACAAA GAAGAAAAAG AGGTGACCGT GAGCTCCGAT
    GAGGAGACTT TCTTTGTCCA GGTCCATGAT GTTTCTCCTG AACAGCCAAG AACGGTTATC
    AAAGCTTTAC GATTCAGTAC AGCGCAGGAA GTTATCCAGC AGGCTTTATG CAAGGCTAAA
    TACTCATACA GCATCCTGAG CAACCCTAAT CCTAGTGATT ATGTGCTTCT GGAGGAAGTC
    ATCAAAGAAG CTTCAAATAA GAAGTCTTCT ACCTCCAAAA CAATTCAGAG GGTTCTCTTG
    GATCAAGAGT GTGTATTCCA GGCACAGAGC AAGTGGAAGG GAGCAGGGAA ATTCATTCTT
    AAACTGAAGG AACAAGTCCA GGCGGCAAGA GACGACAAGC GCAAAGGTCT GTCTATAGCC
    AGTGAACTGA AGAAGCTAGC AAAGTCCAGC CGGCAATCTC GGAACTTCAC TTTGTCACCT
    CAGATATTTT TATCAGAAGA TGCCTGTGAA AGCCCTGAAC CTACAAAAAG CAAAACGCAG
    GAAAAGCCTT CGGCAGATAA TGAAGTAAAT GTGCATCCAA CCTGCTGCAG GAGGTCTTCT
    GCTGCCTGGT ACTTTCTGTT GCTGAAGAAC TGGAAGCTGC CAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761805.1
    CDS566..7573
    Translation

    Target ORF information:

    RefSeq Version XM_031905945.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905945.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCAGTT TATTATGGGA
    GATTTATCTG ACTCTGAAAG TGATATCTTT GAACAATCAA AAGAGTGGGA CTTGCATAGG
    AGGGAAGAGC AACAGAAGGC ATTCAGCCAT GGAACAGAGC TAATTCCCTG GTATGTGTTA
    TCCATGAGAG CAGATGTCTA TCAGTTTCTT CAGCAAGGAG TGACTGTCAT CCGCTATGAC
    CAGGAGACAC ACATTTCTGT TCGTTGTTTC CTTCAACTCC AACCAGACAA CTCCACATTG
    ACTTGGACAA AACCAACCAC AACGTGTCTT GCAAACACTA AAAATAAATT AGGAACTGTA
    AGTAGCAGTG CGGAGATTAA ATTTCAGTTC CTTGCCAATG CTGGAATGAA TGGACTGGCC
    GAAGGATTCT TGGACTTATT CTCTGTAAAG GCAGTTTACA TGGGACATCC GGGAATTGAT
    ATGCACACTG TGTGTGTTCA AAACAAACTA TGTAATATGA ACCTGGAAGA AAATGGTGTC
    ACATTGCTTT ATGGGCTGCA CACTACTGAC AACAAGTTGC TGCACTTTGT AGCTCCGAAA
    TACACAGCCA GGACATTGTA TGATGGACTC CTGGAGTTAA CCAAAGCAGT AAGGAAGATA
    AAACGCTTTC CTGATCAGAG ATTACAGTGG TTAAGGAAGC AGTATGTTAG TCTCTACCAG
    GAGGAAAACA GATTTGAAGG ACCAGCTTTG GCACAAGCTA TTGAGCTGTT TGGAGGGCGA
    CGCTGGAGTA CGAGAAACAC GAGCACAGGG ACCTTAACCA AAAGCACAGA GAAACCAAAC
    GTGCAGAGGA ATAACACTCT CGGCATCAAC ACTGCCAAGA AGAAGAAGAA AGTGCTTATG
    AGGGGCGAGA GTGGGGATGC AGCCGATGAT GAGATGGCAA CCCGCAAAAC AAAAAGCTGC
    AAGGAAAGTC GCAGTAGAAG TGGCTCTGAT CCTCCAGAAA TCGATGAGCA GGAAGAGCAA
    GATCTGAATA TTATTGCTGG ATATTCACCA TCTCAAATGC TCCCATCCCG AAGAGCTCAT
    TCTATGTCGA CTTCTGGTTC TCCCAACTTG ACACCCGGGC CATCTATTCC ACTGCGACCT
    GCCTCCTCTC CTATTTTGTC CAACTCAAAC AAACCCCAGT CCAATACGTG GAGCAGTTCA
    AGTTGGCATG GAAGAGTTAA AGGTGGGATG AAAGGCTTCC AATCATTCAT GGTTTCGGAC
    AGCAACATGA ACTTTACTGA ATTTGTAGAG CTTTTCAAAT CTTTTAGCGT AAGAAGTCGG
    AAAGACCTGA AGGACATTTT TGATGTGTAT GCTGTGGCCT GCAATCGGTC AGGTGCAGAA
    TCAGTCCCTC TCTACACTAA TTTGACAATT GATGAGAATG TAGTTGGAGT CCAACCTGAT
    TTAGATTTAT TGACCAGGAA TGTGTCCGAC TTGGGTTTAT TCATTCGCAG CAGACAGCAA
    CTGTCTGAGA ACCAAAGGCA AATCTCAGAT GCCATTGCGG CTGCCAGCAT TGTAACTAAT
    GGAACTGGAG TAGAAAGCAC ATCCCTGGGC ATATTTGGAA TGGGAATACT GCAGTTTAAT
    GATTTCCTGG TGAATTGCCA GGGTGAACAT TACACCTATG ATGAAGTTCT TAGCATTATT
    CAGAAATTTG AACCAAGTAT CAGTATGCGC CAGCAGGGGC TGATGTCATT TGAAGGATTT
    GCAAGGTTCC TGATGGATAA AGACAATTTT GCATCAAGAA ATGATGAGTC TCAGGTAAAC
    ACAGAAGAAC TTCAGCTGCC CCTTTCCTAC TATTTCATTG AGTCTTCACA CAACACCTAC
    CTTACAGGGC ACCAGCTAAA AGGAGAGTCT TCAGTTGAGC TGTACAGCCA GGTCCTTTTG
    CAGGGGTGCC GAAGTGTTGA ATTAGACTGC TGGGATGGAG ATGACGGCAT GCCTATCATT
    TATCATGGAC ATACACTAAC TACTAAAATT CCCTTTAAGG ATGTCATTGA AGCTATTGGC
    CGAAGTGCAT TTATTACCTC TGAAATGCCA ATCGTACTGT CCATAGAAAA CCATTGCTCC
    CTACCACAGC AGCGTAAAAT GGCAGATATA TTTAAGAATG TCTTTGGTGA AAAACTGGTG
    ACAAAGTTCC TGTTTGAAAG TGACTTTTCA GATGATCCGA TGTTGCCTTC TCCATGGCAA
    TTGAGAAATA AAGTGCTTCT GAAAAATAAA AAGCTGAAGG CACATCAAAC CCCGGTGGAT
    ATACTGAAGC AGAAGGCACA TCAGCTGGCT AGCATGCAAG CACAAGCAAG CAATGGTAGC
    CAAATGGTAA GCCCTAGCAC AAACAATGAA GAAGAAGAAG ATGAAGAAGA TGAATATGAT
    TATGACTATG AGTCTCTGTC AGATGCCGAT GTCCTTAATG CTTCCCCTGC TCCTAACAGC
    CAGGAAGATA ATATTCTTGA TGACCGATCT GAAACTAAAA CCAACAGCGA CAAACTGCAA
    TTTGAATATA ATGAGGAAGC TTCAAAGCGA ATCAAAAAGA CAGATGGCAG CAGTATTAAC
    ACTAAAGGAA AGGTCTATGA CATGGAGTTG GGTGAGGAAT TCTATCTTCC ACAAAACAAA
    AAAGAGAGCA GGCAAATTGC ACCTGAATTG TCAGACCTTA TTATCTATTG CCAAGCTGTC
    AAATTCCCAG GCCTTACAAC ATTAAACCCA TGTGGATCTG GCAGAGGGAA GGAAAGAAAA
    AGCAGGAAGT CCATTTTTGG CAACAACCCT GGCAGAACAA GTCCTGGGGA ACCAACAGCA
    CTTGCCAAAA CATCTGGAAA AGGTACAAAT GAAACACGGC AGAGCTGGGA AGAGCCATGT
    TCTCCTCCCT TTAACCCCAG CACTTCCCTC AGTGCAATAA TCAGGACACC CAGGTGCTAC
    CATATCTCTT CCCTAAATGA GAACGCAGCC AAGCGCTTGT GCCGAAGGTA TTCTCAGAAA
    CTCATTCAAC ATACCGCATA TCAACTGCTC CGAACATATC CCGCCGCCAC TCGGATAGAC
    TCTTCCAACC CACATCCTCT TATTTTCTGG CTTCATGGTG TACAGTTGGT TGCTCTAAAC
    TACCAAACTG ATGATCTCCC CTTACATCTA AACGCCGCCA TGTTTGAAGC AAATGGTGGT
    TGCGGATATG TGTTAAAGCC CCCCGTGCAG TGGGATAGGA CCTGCCCAAT GTACCAATTA
    TTCTCACCAT TAGAACGAGA TTTAGAAAAT ATGGAACCAG CAATATATTC TTTGACGATA
    GTCTCTGGGC AAAATGTGTG TCCAGGTAAC AGCTCAGGCA GCCCCTGCAT TGAAATTGAT
    GTCTTGGGTA TGCCAGTAGA CAGCTGCCAT TTTCGGACCA AACCAATCCA TCGCAATACC
    TTAAATCCCA TGTGGAATGA ACAATTCTTA TTCCGAATAT ACTTTGAGGA TCTTGTGTTT
    CTTCGGTTTG CTGTGGTGGA AAACAACAGC TCTGCTGTAA CTGCCCAAAG AATAATTAAT
    CTTAAAGCTT TAAAGAGAGG TAACAGACAC CTTCAGCTAA GAAATTTACA TAATGAGCCC
    TTAGAGGTTT CTACCTTATT TATAAATAGC AGGAGAATGG AAGAAATCCC TAATGGAAAT
    ACCTTACCCG CCTCCTTGTT TTTCAGCTCA GAAGAAAGAA AGACTCCAGC CACATTTAAA
    GCTACAGTAC ATGGGATTCC TGGGCCAGAA CCATTTACAG TGTTTGAAAT CAGTCTTGGG
    ACAACTGCAA AGCAACTTCT TGATCATATT TTGGCTACTA TACAAGGCAA TGAAGCAGAC
    ATCACTGACT ATTTTTTGAT GGAGGAAAAA TGCTTTATAT CTAAAGACAA AAATGAATGC
    AAGAAATTGC CGTTCCAGAG AGTGATTAGC CCAGAAGAGG ATATCCTGCA AATTTTGAAT
    AGCTGGTTCC CAGAAGAAGG CTACGTGGGG AGAATTGTTC TTAAAACACG AGAGGAGAAC
    ATGAATGACA AAAATGTTCA AGAGGACAAA GAAGAAAAAG AGGTGACCGT GAGCTCCGAT
    GAGGAGACTT TCTTTGTCCA GGTCCATGAT GTTTCTCCTG AACAGCCAAG AACGGTTATC
    AAAGCTTTAC GATTCAGTAC AGCGCAGGAA GTTATCCAGC AGGCTTTATG CAAGGCTAAA
    TACTCATACA GCATCCTGAG CAACCCTAAT CCTAGTGATT ATGTGCTTCT GGAGGAAGTC
    ATCAAAGAAG CTTCAAATAA GAAGTCTTCT ACCTCCAAAA CAATTCAGAG GGTTCTCTTG
    GATCAAGAGT GTGTATTCCA GGCACAGAGC AAGTGGAAGG GAGCAGGGAA ATTCATTCTT
    AAACTGAAGG AACAAGTCCA GGCGGCAAGA GACGACAAGC GCAAAGGTCT GTCTATAGCC
    AGTGAACTGA AGAAGCTAGC AAAGTCCAGC CGGCAATCTC GGAACTTCAC TTTGTCACCT
    CAGATATTTT TATCAGAAGA TGCCTGTGAA AGCCCTGAAC CTACAAAAAG CAAAACGCAG
    GAAAAGCCTT CGGCAGATAA TGAAGTAAAT GTGCATCCAA CCTGCTGCAG GAGGTCTTCT
    GCTGCCTGGT ACTTTCTGTT GCTGAAGAAC TGGAAGCTGC CAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62380
    Clone ID Related Accession (Same CDS sequence) XM_031905946.1
    Accession Version XM_031905946.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCACTC CAAGAAGGTC
    TATGAGCCAA ACCGGGTTCT GTTTATTATG GGAGATTTAT CTGACTCTGA AAGTGATATC
    TTTGAACAAT CAAAAGAGTG GGACTTGCAT AGGAGGGAAG AGCAACAGAA GGCATTCAGC
    CATGGAACAG AGCTAATTCC CTGGTATGTG TTATCCATGA GAGCAGATGT CTATCAGTTT
    CTTCAGCAAG GAGTGACTGT CATCCGCTAT GACCAGGAGA CACACATTTC TGTTCGTTGT
    TTCCTTCAAC TCCAACCAGA CAACTCCACA TTGACTTGGA CAAAACCAAC CACAACGTGT
    CTTGCAAACA CTAAAAATAA ATTAGGAACT GTAAGTAGCA GTGCGGAGAT TAAATTTCAG
    TTCCTTGCCA ATGCTGGAAT GAATGGACTG GCCGAAGGAT TCTTGGACTT ATTCTCTGTA
    AAGGCAGTTT ACATGGGACA TCCGGGAATT GATATGCACA CTGTGTGTGT TCAAAACAAA
    CTATGTAATA TGAACCTGGA AGAAAATGGT GTCACATTGC TTTATGGGCT GCACACTACT
    GACAACAAGT TGCTGCACTT TGTAGCTCCG AAATACACAG CCAGGACATT GTATGATGGA
    CTCCTGGAGT TAACCAAAGC AGTAAGGAAG ATAAAACGCT TTCCTGATCA GAGATTACAG
    TGGTTAAGGA AGCAGTATGT TAGTCTCTAC CAGGAGGAAA ACAGATTTGA AGGACCAGCT
    TTGGCACAAG CTATTGAGCT GTTTGGAGGG CGACGCTGGA GTACGAGAAA CACGAGCACA
    GGGACCTTAA CCAAAAGCAC AGAGAAACCA AACGTGCAGA GGAATAACAC TCTCGGCATC
    AACACTGCCA AGAAGAAGAA GAAAGTGCTT ATGAGGGGCG AGAGTGGGGA TGCAGCCGAT
    GATGAGATGG CAACCCGCAA AACAAAAAGC TGCAAGGAAA GTCGCAGTAG AAGTGGCTCT
    GATCCTCCAG AAATCGATGA GCAGGAAGAG CAAGATCTGA ATATTATTGC TGGATATTCA
    CCATCTCAAA TGCTCCCATC CCGAAGAGCT CATTCTATGT CGACTTCTGG TTCTCCCAAC
    TTGACACCCG GGCCATCTAT TCCACTGCGA CCTGCCTCCT CTCCTATTTT GTCCAACTCA
    AACAAACCCC AGTCCAATAC GTGGAGCAGT TCAAGTTGGC ATGGAAGAGT TAAAGGTGGG
    ATGAAAGGCT TCCAATCATT CATGGTTTCG GACAGCAACA TGAACTTTAC TGAATTTGTA
    GAGCTTTTCA AATCTTTTAG CGTAAGAAGT CGGAAAGACC TGAAGGACAT TTTTGATGTG
    TATGCTGTGG CCTGCAATCG GTCAGGTGCA GAATCAGTCC CTCTCTACAC TAATTTGACA
    ATTGATGAGA ATGTAGTTGG AGTCCAACCT GATTTAGATT TATTGACCAG GAATGTGTCC
    GACTTGGGTT TATTCATTCG CAGCAGACAG CAACTGTCTG AGAACCAAAG GCAAATCTCA
    GATGCCATTG CGGCTGCCAG CATTGTAACT AATGGAACTG GAGTAGAAAG CACATCCCTG
    GGCATATTTG GAATGGGAAT ACTGCAGTTT AATGATTTCC TGGTGAATTG CCAGGGTGAA
    CATTACACCT ATGATGAAGT TCTTAGCATT ATTCAGAAAT TTGAACCAAG TATCAGTATG
    CGCCAGCAGG GGCTGATGTC ATTTGAAGGA TTTGCAAGGT TCCTGATGGA TAAAGACAAT
    TTTGCATCAA GAAATGATGA GTCTCAGGTA AACACAGAAG AACTTCAGCT GCCCCTTTCC
    TACTATTTCA TTGAGTCTTC ACACAACACC TACCTTACAG GGCACCAGCT AAAAGGAGAG
    TCTTCAGTTG AGCTGTACAG CCAGGTCCTT TTGCAGGGGT GCCGAAGTGT TGAATTAGAC
    TGCTGGGATG GAGATGACGG CATGCCTATC ATTTATCATG GACATACACT AACTACTAAA
    ATTCCCTTTA AGGATGTCAT TGAAGCTATT GGCCGAAGTG CATTTATTAC CTCTGAAATG
    CCAATCGTAC TGTCCATAGA AAACCATTGC TCCCTACCAC AGCAGCGTAA AATGGCAGAT
    ATATTTAAGA ATGTCTTTGG TGAAAAACTG GTGACAAAGT TCCTGTTTGA AAGTGACTTT
    TCAGATGATC CGATGTTGCC TTCTCCATGG CAATTGAGAA ATAAAGTGCT TCTGAAAAAT
    AAAAAGCTGA AGGCACATCA AACCCCGGTG GATATACTGA AGCAGAAGGC ACATCAGCTG
    GCTAGCATGC AAGCACAAGC AAGCAATGGT AGCCAAATGG TAAGCCCTAG CACAAACAAT
    GAAGAAGAAG AAGATGAAGA AGATGAATAT GATTATGACT ATGAGTCTCT GTCAGATGAT
    AATATTCTTG ATGACCGATC TGAAACTAAA ACCAACAGCG ACAAACTGCA ATTTGAATAT
    AATGAGGAAG CTTCAAAGCG AATCAAAAAG ACAGATGGCA GCAGTATTAA CACTAAAGGA
    AAGGTCTATG ACATGGAGTT GGGTGAGGAA TTCTATCTTC CACAAAACAA AAAAGAGAGC
    AGGCAAATTG CACCTGAATT GTCAGACCTT ATTATCTATT GCCAAGCTGT CAAATTCCCA
    GGCCTTACAA CATTAAACCC ATGTGGATCT GGCAGAGGGA AGGAAAGAAA AAGCAGGAAG
    TCCATTTTTG GCAACAACCC TGGCAGAACA AGTCCTGGGG AACCAACAGC ACTTGCCAAA
    ACATCTGGAA AAGGTACAAA TGAAACACGG CAGAGCTGGG AAGAGCCATG TTCTCCTCCC
    TTTAACCCCA GCACTTCCCT CAGTGCAATA ATCAGGACAC CCAGGTGCTA CCATATCTCT
    TCCCTAAATG AGAACGCAGC CAAGCGCTTG TGCCGAAGGT ATTCTCAGAA ACTCATTCAA
    CATACCGCAT ATCAACTGCT CCGAACATAT CCCGCCGCCA CTCGGATAGA CTCTTCCAAC
    CCACATCCTC TTATTTTCTG GCTTCATGGT GTACAGTTGG TTGCTCTAAA CTACCAAACT
    GATGATCTCC CCTTACATCT AAACGCCGCC ATGTTTGAAG CAAATGGTGG TTGCGGATAT
    GTGTTAAAGC CCCCCGTGCA GTGGGATAGG ACCTGCCCAA TGTACCAATT ATTCTCACCA
    TTAGAACGAG ATTTAGAAAA TATGGAACCA GCAATATATT CTTTGACGAT AGTCTCTGGG
    CAAAATGTGT GTCCAGGTAA CAGCTCAGGC AGCCCCTGCA TTGAAATTGA TGTCTTGGGT
    ATGCCAGTAG ACAGCTGCCA TTTTCGGACC AAACCAATCC ATCGCAATAC CTTAAATCCC
    ATGTGGAATG AACAATTCTT ATTCCGAATA TACTTTGAGG ATCTTGTGTT TCTTCGGTTT
    GCTGTGGTGG AAAACAACAG CTCTGCTGTA ACTGCCCAAA GAATAATTAA TCTTAAAGCT
    TTAAAGAGAG GTAACAGACA CCTTCAGCTA AGAAATTTAC ATAATGAGCC CTTAGAGGTT
    TCTACCTTAT TTATAAATAG CAGGAGAATG GAAGAAATCC CTAATGGAAA TACCTTACCC
    GCCTCCTTGT TTTTCAGCTC AGAAGAAAGA AAGACTCCAG CCACATTTAA AGCTACAGTA
    CATGGGATTC CTGGGCCAGA ACCATTTACA GTGTTTGAAA TCAGTCTTGG GACAACTGCA
    AAGCAACTTC TTGATCATAT TTTGGCTACT ATACAAGGCA ATGAAGCAGA CATCACTGAC
    TATTTTTTGA TGGAGGAAAA ATGCTTTATA TCTAAAGACA AAAATGAATG CAAGAAATTG
    CCGTTCCAGA GAGTGATTAG CCCAGAAGAG GATATCCTGC AAATTTTGAA TAGCTGGTTC
    CCAGAAGAAG GCTACGTGGG GAGAATTGTT CTTAAAACAC GAGAGGAGAA CATGAATGAC
    AAAAATGTTC AAGAGGACAA AGAAGAAAAA GAGGTGACCG TGAGCTCCGA TGAGGAGACT
    TTCTTTGTCC AGGTCCATGA TGTTTCTCCT GAACAGCCAA GAACGGTTAT CAAAGCTTTA
    CGATTCAGTA CAGCGCAGGA AGTTATCCAG CAGGCTTTAT GCAAGGCTAA ATACTCATAC
    AGCATCCTGA GCAACCCTAA TCCTAGTGAT TATGTGCTTC TGGAGGAAGT CATCAAAGAA
    GCTTCAAATA AGAAGTCTTC TACCTCCAAA ACAATTCAGA GGGTTCTCTT GGATCAAGAG
    TGTGTATTCC AGGCACAGAG CAAGTGGAAG GGAGCAGGGA AATTCATTCT TAAACTGAAG
    GAACAAGTCC AGGCGGCAAG AGACGACAAG CGCAAAGGTC TGTCTATAGC CAGTGAACTG
    AAGAAGCTAG CAAAGTCCAG CCGGCAATCT CGGAACTTCA CTTTGTCACC TCAGATATTT
    TTATCAGAAG ATGCCTGTGA AAGCCCTGAA CCTACAAAAA GCAAAACGCA GGAAAAGCCT
    TCGGCAGATA ATGAAGTAAA TGTGCATCCA ACCTGCTGCA GGAGGTCTTC TGCTGCCTGG
    TACTTTCTGT TGCTGAAGAA CTGGAAGCTG CCAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761806.1
    CDS566..7564
    Translation

    Target ORF information:

    RefSeq Version XM_031905946.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905946.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCACTC CAAGAAGGTC
    TATGAGCCAA ACCGGGTTCT GTTTATTATG GGAGATTTAT CTGACTCTGA AAGTGATATC
    TTTGAACAAT CAAAAGAGTG GGACTTGCAT AGGAGGGAAG AGCAACAGAA GGCATTCAGC
    CATGGAACAG AGCTAATTCC CTGGTATGTG TTATCCATGA GAGCAGATGT CTATCAGTTT
    CTTCAGCAAG GAGTGACTGT CATCCGCTAT GACCAGGAGA CACACATTTC TGTTCGTTGT
    TTCCTTCAAC TCCAACCAGA CAACTCCACA TTGACTTGGA CAAAACCAAC CACAACGTGT
    CTTGCAAACA CTAAAAATAA ATTAGGAACT GTAAGTAGCA GTGCGGAGAT TAAATTTCAG
    TTCCTTGCCA ATGCTGGAAT GAATGGACTG GCCGAAGGAT TCTTGGACTT ATTCTCTGTA
    AAGGCAGTTT ACATGGGACA TCCGGGAATT GATATGCACA CTGTGTGTGT TCAAAACAAA
    CTATGTAATA TGAACCTGGA AGAAAATGGT GTCACATTGC TTTATGGGCT GCACACTACT
    GACAACAAGT TGCTGCACTT TGTAGCTCCG AAATACACAG CCAGGACATT GTATGATGGA
    CTCCTGGAGT TAACCAAAGC AGTAAGGAAG ATAAAACGCT TTCCTGATCA GAGATTACAG
    TGGTTAAGGA AGCAGTATGT TAGTCTCTAC CAGGAGGAAA ACAGATTTGA AGGACCAGCT
    TTGGCACAAG CTATTGAGCT GTTTGGAGGG CGACGCTGGA GTACGAGAAA CACGAGCACA
    GGGACCTTAA CCAAAAGCAC AGAGAAACCA AACGTGCAGA GGAATAACAC TCTCGGCATC
    AACACTGCCA AGAAGAAGAA GAAAGTGCTT ATGAGGGGCG AGAGTGGGGA TGCAGCCGAT
    GATGAGATGG CAACCCGCAA AACAAAAAGC TGCAAGGAAA GTCGCAGTAG AAGTGGCTCT
    GATCCTCCAG AAATCGATGA GCAGGAAGAG CAAGATCTGA ATATTATTGC TGGATATTCA
    CCATCTCAAA TGCTCCCATC CCGAAGAGCT CATTCTATGT CGACTTCTGG TTCTCCCAAC
    TTGACACCCG GGCCATCTAT TCCACTGCGA CCTGCCTCCT CTCCTATTTT GTCCAACTCA
    AACAAACCCC AGTCCAATAC GTGGAGCAGT TCAAGTTGGC ATGGAAGAGT TAAAGGTGGG
    ATGAAAGGCT TCCAATCATT CATGGTTTCG GACAGCAACA TGAACTTTAC TGAATTTGTA
    GAGCTTTTCA AATCTTTTAG CGTAAGAAGT CGGAAAGACC TGAAGGACAT TTTTGATGTG
    TATGCTGTGG CCTGCAATCG GTCAGGTGCA GAATCAGTCC CTCTCTACAC TAATTTGACA
    ATTGATGAGA ATGTAGTTGG AGTCCAACCT GATTTAGATT TATTGACCAG GAATGTGTCC
    GACTTGGGTT TATTCATTCG CAGCAGACAG CAACTGTCTG AGAACCAAAG GCAAATCTCA
    GATGCCATTG CGGCTGCCAG CATTGTAACT AATGGAACTG GAGTAGAAAG CACATCCCTG
    GGCATATTTG GAATGGGAAT ACTGCAGTTT AATGATTTCC TGGTGAATTG CCAGGGTGAA
    CATTACACCT ATGATGAAGT TCTTAGCATT ATTCAGAAAT TTGAACCAAG TATCAGTATG
    CGCCAGCAGG GGCTGATGTC ATTTGAAGGA TTTGCAAGGT TCCTGATGGA TAAAGACAAT
    TTTGCATCAA GAAATGATGA GTCTCAGGTA AACACAGAAG AACTTCAGCT GCCCCTTTCC
    TACTATTTCA TTGAGTCTTC ACACAACACC TACCTTACAG GGCACCAGCT AAAAGGAGAG
    TCTTCAGTTG AGCTGTACAG CCAGGTCCTT TTGCAGGGGT GCCGAAGTGT TGAATTAGAC
    TGCTGGGATG GAGATGACGG CATGCCTATC ATTTATCATG GACATACACT AACTACTAAA
    ATTCCCTTTA AGGATGTCAT TGAAGCTATT GGCCGAAGTG CATTTATTAC CTCTGAAATG
    CCAATCGTAC TGTCCATAGA AAACCATTGC TCCCTACCAC AGCAGCGTAA AATGGCAGAT
    ATATTTAAGA ATGTCTTTGG TGAAAAACTG GTGACAAAGT TCCTGTTTGA AAGTGACTTT
    TCAGATGATC CGATGTTGCC TTCTCCATGG CAATTGAGAA ATAAAGTGCT TCTGAAAAAT
    AAAAAGCTGA AGGCACATCA AACCCCGGTG GATATACTGA AGCAGAAGGC ACATCAGCTG
    GCTAGCATGC AAGCACAAGC AAGCAATGGT AGCCAAATGG TAAGCCCTAG CACAAACAAT
    GAAGAAGAAG AAGATGAAGA AGATGAATAT GATTATGACT ATGAGTCTCT GTCAGATGAT
    AATATTCTTG ATGACCGATC TGAAACTAAA ACCAACAGCG ACAAACTGCA ATTTGAATAT
    AATGAGGAAG CTTCAAAGCG AATCAAAAAG ACAGATGGCA GCAGTATTAA CACTAAAGGA
    AAGGTCTATG ACATGGAGTT GGGTGAGGAA TTCTATCTTC CACAAAACAA AAAAGAGAGC
    AGGCAAATTG CACCTGAATT GTCAGACCTT ATTATCTATT GCCAAGCTGT CAAATTCCCA
    GGCCTTACAA CATTAAACCC ATGTGGATCT GGCAGAGGGA AGGAAAGAAA AAGCAGGAAG
    TCCATTTTTG GCAACAACCC TGGCAGAACA AGTCCTGGGG AACCAACAGC ACTTGCCAAA
    ACATCTGGAA AAGGTACAAA TGAAACACGG CAGAGCTGGG AAGAGCCATG TTCTCCTCCC
    TTTAACCCCA GCACTTCCCT CAGTGCAATA ATCAGGACAC CCAGGTGCTA CCATATCTCT
    TCCCTAAATG AGAACGCAGC CAAGCGCTTG TGCCGAAGGT ATTCTCAGAA ACTCATTCAA
    CATACCGCAT ATCAACTGCT CCGAACATAT CCCGCCGCCA CTCGGATAGA CTCTTCCAAC
    CCACATCCTC TTATTTTCTG GCTTCATGGT GTACAGTTGG TTGCTCTAAA CTACCAAACT
    GATGATCTCC CCTTACATCT AAACGCCGCC ATGTTTGAAG CAAATGGTGG TTGCGGATAT
    GTGTTAAAGC CCCCCGTGCA GTGGGATAGG ACCTGCCCAA TGTACCAATT ATTCTCACCA
    TTAGAACGAG ATTTAGAAAA TATGGAACCA GCAATATATT CTTTGACGAT AGTCTCTGGG
    CAAAATGTGT GTCCAGGTAA CAGCTCAGGC AGCCCCTGCA TTGAAATTGA TGTCTTGGGT
    ATGCCAGTAG ACAGCTGCCA TTTTCGGACC AAACCAATCC ATCGCAATAC CTTAAATCCC
    ATGTGGAATG AACAATTCTT ATTCCGAATA TACTTTGAGG ATCTTGTGTT TCTTCGGTTT
    GCTGTGGTGG AAAACAACAG CTCTGCTGTA ACTGCCCAAA GAATAATTAA TCTTAAAGCT
    TTAAAGAGAG GTAACAGACA CCTTCAGCTA AGAAATTTAC ATAATGAGCC CTTAGAGGTT
    TCTACCTTAT TTATAAATAG CAGGAGAATG GAAGAAATCC CTAATGGAAA TACCTTACCC
    GCCTCCTTGT TTTTCAGCTC AGAAGAAAGA AAGACTCCAG CCACATTTAA AGCTACAGTA
    CATGGGATTC CTGGGCCAGA ACCATTTACA GTGTTTGAAA TCAGTCTTGG GACAACTGCA
    AAGCAACTTC TTGATCATAT TTTGGCTACT ATACAAGGCA ATGAAGCAGA CATCACTGAC
    TATTTTTTGA TGGAGGAAAA ATGCTTTATA TCTAAAGACA AAAATGAATG CAAGAAATTG
    CCGTTCCAGA GAGTGATTAG CCCAGAAGAG GATATCCTGC AAATTTTGAA TAGCTGGTTC
    CCAGAAGAAG GCTACGTGGG GAGAATTGTT CTTAAAACAC GAGAGGAGAA CATGAATGAC
    AAAAATGTTC AAGAGGACAA AGAAGAAAAA GAGGTGACCG TGAGCTCCGA TGAGGAGACT
    TTCTTTGTCC AGGTCCATGA TGTTTCTCCT GAACAGCCAA GAACGGTTAT CAAAGCTTTA
    CGATTCAGTA CAGCGCAGGA AGTTATCCAG CAGGCTTTAT GCAAGGCTAA ATACTCATAC
    AGCATCCTGA GCAACCCTAA TCCTAGTGAT TATGTGCTTC TGGAGGAAGT CATCAAAGAA
    GCTTCAAATA AGAAGTCTTC TACCTCCAAA ACAATTCAGA GGGTTCTCTT GGATCAAGAG
    TGTGTATTCC AGGCACAGAG CAAGTGGAAG GGAGCAGGGA AATTCATTCT TAAACTGAAG
    GAACAAGTCC AGGCGGCAAG AGACGACAAG CGCAAAGGTC TGTCTATAGC CAGTGAACTG
    AAGAAGCTAG CAAAGTCCAG CCGGCAATCT CGGAACTTCA CTTTGTCACC TCAGATATTT
    TTATCAGAAG ATGCCTGTGA AAGCCCTGAA CCTACAAAAA GCAAAACGCA GGAAAAGCCT
    TCGGCAGATA ATGAAGTAAA TGTGCATCCA ACCTGCTGCA GGAGGTCTTC TGCTGCCTGG
    TACTTTCTGT TGCTGAAGAA CTGGAAGCTG CCAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62381
    Clone ID Related Accession (Same CDS sequence) XM_031905947.1
    Accession Version XM_031905947.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCAGTT TATTATGGGA
    GATTTATCTG ACTCTGAAAG TGATATCTTT GAACAATCAA AAGAGTGGGA CTTGCATAGG
    AGGGAAGAGC AACAGAAGGC ATTCAGCCAT GGAACAGAGC TAATTCCCTG GTATGTGTTA
    TCCATGAGAG CAGATGTCTA TCAGTTTCTT CAGCAAGGAG TGACTGTCAT CCGCTATGAC
    CAGGAGACAC ACATTTCTGT TCGTTGTTTC CTTCAACTCC AACCAGACAA CTCCACATTG
    ACTTGGACAA AACCAACCAC AACGTGTCTT GCAAACACTA AAAATAAATT AGGAACTGTA
    AGTAGCAGTG CGGAGATTAA ATTTCAGTTC CTTGCCAATG CTGGAATGAA TGGACTGGCC
    GAAGGATTCT TGGACTTATT CTCTGTAAAG GCAGTTTACA TGGGACATCC GGGAATTGAT
    ATGCACACTG TGTGTGTTCA AAACAAACTA TGTAATATGA ACCTGGAAGA AAATGGTGTC
    ACATTGCTTT ATGGGCTGCA CACTACTGAC AACAAGTTGC TGCACTTTGT AGCTCCGAAA
    TACACAGCCA GGACATTGTA TGATGGACTC CTGGAGTTAA CCAAAGCAGT AAGGAAGATA
    AAACGCTTTC CTGATCAGAG ATTACAGTGG TTAAGGAAGC AGTATGTTAG TCTCTACCAG
    GAGGAAAACA GATTTGAAGG ACCAGCTTTG GCACAAGCTA TTGAGCTGTT TGGAGGGCGA
    CGCTGGAGTA CGAGAAACAC GAGCACAGGG ACCTTAACCA AAAGCACAGA GAAACCAAAC
    GTGCAGAGGA ATAACACTCT CGGCATCAAC ACTGCCAAGA AGAAGAAGAA AGTGCTTATG
    AGGGGCGAGA GTGGGGATGC AGCCGATGAT GAGATGGCAA CCCGCAAAAC AAAAAGCTGC
    AAGGAAAGTC GCAGTAGAAG TGGCTCTGAT CCTCCAGAAA TCGATGAGCA GGAAGAGCAA
    GATCTGAATA TTATTGCTGG ATATTCACCA TCTCAAATGC TCCCATCCCG AAGAGCTCAT
    TCTATGTCGA CTTCTGGTTC TCCCAACTTG ACACCCGGGC CATCTATTCC ACTGCGACCT
    GCCTCCTCTC CTATTTTGTC CAACTCAAAC AAACCCCAGT CCAATACGTG GAGCAGTTCA
    AGTTGGCATG GAAGAGTTAA AGGTGGGATG AAAGGCTTCC AATCATTCAT GGTTTCGGAC
    AGCAACATGA ACTTTACTGA ATTTGTAGAG CTTTTCAAAT CTTTTAGCGT AAGAAGTCGG
    AAAGACCTGA AGGACATTTT TGATGTGTAT GCTGTGGCCT GCAATCGGTC AGGTGCAGAA
    TCAGTCCCTC TCTACACTAA TTTGACAATT GATGAGAATG TAGTTGGAGT CCAACCTGAT
    TTAGATTTAT TGACCAGGAA TGTGTCCGAC TTGGGTTTAT TCATTCGCAG CAGACAGCAA
    CTGTCTGAGA ACCAAAGGCA AATCTCAGAT GCCATTGCGG CTGCCAGCAT TGTAACTAAT
    GGAACTGGAG TAGAAAGCAC ATCCCTGGGC ATATTTGGAA TGGGAATACT GCAGTTTAAT
    GATTTCCTGG TGAATTGCCA GGGTGAACAT TACACCTATG ATGAAGTTCT TAGCATTATT
    CAGAAATTTG AACCAAGTAT CAGTATGCGC CAGCAGGGGC TGATGTCATT TGAAGGATTT
    GCAAGGTTCC TGATGGATAA AGACAATTTT GCATCAAGAA ATGATGAGTC TCAGGTAAAC
    ACAGAAGAAC TTCAGCTGCC CCTTTCCTAC TATTTCATTG AGTCTTCACA CAACACCTAC
    CTTACAGGGC ACCAGCTAAA AGGAGAGTCT TCAGTTGAGC TGTACAGCCA GGTCCTTTTG
    CAGGGGTGCC GAAGTGTTGA ATTAGACTGC TGGGATGGAG ATGACGGCAT GCCTATCATT
    TATCATGGAC ATACACTAAC TACTAAAATT CCCTTTAAGG ATGTCATTGA AGCTATTGGC
    CGAAGTGCAT TTATTACCTC TGAAATGCCA ATCGTACTGT CCATAGAAAA CCATTGCTCC
    CTACCACAGC AGCGTAAAAT GGCAGATATA TTTAAGAATG TCTTTGGTGA AAAACTGGTG
    ACAAAGTTCC TGTTTGAAAG TGACTTTTCA GATGATCCGA TGTTGCCTTC TCCATGGCAA
    TTGAGAAATA AAGTGCTTCT GAAAAATAAA AAGCTGAAGG CACATCAAAC CCCGGTGGAT
    ATACTGAAGC AGAAGGCACA TCAGCTGGCT AGCATGCAAG CACAAGCAAG CAATGGTAGC
    CAAATGGTAA GCCCTAGCAC AAACAATGAA GAAGAAGAAG ATGAAGAAGA TGAATATGAT
    TATGACTATG AGTCTCTGTC AGATGATAAT ATTCTTGATG ACCGATCTGA AACTAAAACC
    AACAGCGACA AACTGCAATT TGAATATAAT GAGGAAGCTT CAAAGCGAAT CAAAAAGACA
    GATGGCAGCA GTATTAACAC TAAAGGAAAG GTCTATGACA TGGAGTTGGG TGAGGAATTC
    TATCTTCCAC AAAACAAAAA AGAGAGCAGG CAAATTGCAC CTGAATTGTC AGACCTTATT
    ATCTATTGCC AAGCTGTCAA ATTCCCAGGC CTTACAACAT TAAACCCATG TGGATCTGGC
    AGAGGGAAGG AAAGAAAAAG CAGGAAGTCC ATTTTTGGCA ACAACCCTGG CAGAACAAGT
    CCTGGGGAAC CAACAGCACT TGCCAAAACA TCTGGAAAAG GTACAAATGA AACACGGCAG
    AGCTGGGAAG AGCCATGTTC TCCTCCCTTT AACCCCAGCA CTTCCCTCAG TGCAATAATC
    AGGACACCCA GGTGCTACCA TATCTCTTCC CTAAATGAGA ACGCAGCCAA GCGCTTGTGC
    CGAAGGTATT CTCAGAAACT CATTCAACAT ACCGCATATC AACTGCTCCG AACATATCCC
    GCCGCCACTC GGATAGACTC TTCCAACCCA CATCCTCTTA TTTTCTGGCT TCATGGTGTA
    CAGTTGGTTG CTCTAAACTA CCAAACTGAT GATCTCCCCT TACATCTAAA CGCCGCCATG
    TTTGAAGCAA ATGGTGGTTG CGGATATGTG TTAAAGCCCC CCGTGCAGTG GGATAGGACC
    TGCCCAATGT ACCAATTATT CTCACCATTA GAACGAGATT TAGAAAATAT GGAACCAGCA
    ATATATTCTT TGACGATAGT CTCTGGGCAA AATGTGTGTC CAGGTAACAG CTCAGGCAGC
    CCCTGCATTG AAATTGATGT CTTGGGTATG CCAGTAGACA GCTGCCATTT TCGGACCAAA
    CCAATCCATC GCAATACCTT AAATCCCATG TGGAATGAAC AATTCTTATT CCGAATATAC
    TTTGAGGATC TTGTGTTTCT TCGGTTTGCT GTGGTGGAAA ACAACAGCTC TGCTGTAACT
    GCCCAAAGAA TAATTAATCT TAAAGCTTTA AAGAGAGGTA ACAGACACCT TCAGCTAAGA
    AATTTACATA ATGAGCCCTT AGAGGTTTCT ACCTTATTTA TAAATAGCAG GAGAATGGAA
    GAAATCCCTA ATGGAAATAC CTTACCCGCC TCCTTGTTTT TCAGCTCAGA AGAAAGAAAG
    ACTCCAGCCA CATTTAAAGC TACAGTACAT GGGATTCCTG GGCCAGAACC ATTTACAGTG
    TTTGAAATCA GTCTTGGGAC AACTGCAAAG CAACTTCTTG ATCATATTTT GGCTACTATA
    CAAGGCAATG AAGCAGACAT CACTGACTAT TTTTTGATGG AGGAAAAATG CTTTATATCT
    AAAGACAAAA ATGAATGCAA GAAATTGCCG TTCCAGAGAG TGATTAGCCC AGAAGAGGAT
    ATCCTGCAAA TTTTGAATAG CTGGTTCCCA GAAGAAGGCT ACGTGGGGAG AATTGTTCTT
    AAAACACGAG AGGAGAACAT GAATGACAAA AATGTTCAAG AGGACAAAGA AGAAAAAGAG
    GTGACCGTGA GCTCCGATGA GGAGACTTTC TTTGTCCAGG TCCATGATGT TTCTCCTGAA
    CAGCCAAGAA CGGTTATCAA AGCTTTACGA TTCAGTACAG CGCAGGAAGT TATCCAGCAG
    GCTTTATGCA AGGCTAAATA CTCATACAGC ATCCTGAGCA ACCCTAATCC TAGTGATTAT
    GTGCTTCTGG AGGAAGTCAT CAAAGAAGCT TCAAATAAGA AGTCTTCTAC CTCCAAAACA
    ATTCAGAGGG TTCTCTTGGA TCAAGAGTGT GTATTCCAGG CACAGAGCAA GTGGAAGGGA
    GCAGGGAAAT TCATTCTTAA ACTGAAGGAA CAAGTCCAGG CGGCAAGAGA CGACAAGCGC
    AAAGGTCTGT CTATAGCCAG TGAACTGAAG AAGCTAGCAA AGTCCAGCCG GCAATCTCGG
    AACTTCACTT TGTCACCTCA GATATTTTTA TCAGAAGATG CCTGTGAAAG CCCTGAACCT
    ACAAAAAGCA AAACGCAGGA AAAGCCTTCG GCAGATAATG AAGTAAATGT GCATCCAACC
    TGCTGCAGGA GGTCTTCTGC TGCCTGGTAC TTTCTGTTGC TGAAGAACTG GAAGCTGCCA
    AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761807.1
    CDS566..7531
    Translation

    Target ORF information:

    RefSeq Version XM_031905947.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905947.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCAGTT TATTATGGGA
    GATTTATCTG ACTCTGAAAG TGATATCTTT GAACAATCAA AAGAGTGGGA CTTGCATAGG
    AGGGAAGAGC AACAGAAGGC ATTCAGCCAT GGAACAGAGC TAATTCCCTG GTATGTGTTA
    TCCATGAGAG CAGATGTCTA TCAGTTTCTT CAGCAAGGAG TGACTGTCAT CCGCTATGAC
    CAGGAGACAC ACATTTCTGT TCGTTGTTTC CTTCAACTCC AACCAGACAA CTCCACATTG
    ACTTGGACAA AACCAACCAC AACGTGTCTT GCAAACACTA AAAATAAATT AGGAACTGTA
    AGTAGCAGTG CGGAGATTAA ATTTCAGTTC CTTGCCAATG CTGGAATGAA TGGACTGGCC
    GAAGGATTCT TGGACTTATT CTCTGTAAAG GCAGTTTACA TGGGACATCC GGGAATTGAT
    ATGCACACTG TGTGTGTTCA AAACAAACTA TGTAATATGA ACCTGGAAGA AAATGGTGTC
    ACATTGCTTT ATGGGCTGCA CACTACTGAC AACAAGTTGC TGCACTTTGT AGCTCCGAAA
    TACACAGCCA GGACATTGTA TGATGGACTC CTGGAGTTAA CCAAAGCAGT AAGGAAGATA
    AAACGCTTTC CTGATCAGAG ATTACAGTGG TTAAGGAAGC AGTATGTTAG TCTCTACCAG
    GAGGAAAACA GATTTGAAGG ACCAGCTTTG GCACAAGCTA TTGAGCTGTT TGGAGGGCGA
    CGCTGGAGTA CGAGAAACAC GAGCACAGGG ACCTTAACCA AAAGCACAGA GAAACCAAAC
    GTGCAGAGGA ATAACACTCT CGGCATCAAC ACTGCCAAGA AGAAGAAGAA AGTGCTTATG
    AGGGGCGAGA GTGGGGATGC AGCCGATGAT GAGATGGCAA CCCGCAAAAC AAAAAGCTGC
    AAGGAAAGTC GCAGTAGAAG TGGCTCTGAT CCTCCAGAAA TCGATGAGCA GGAAGAGCAA
    GATCTGAATA TTATTGCTGG ATATTCACCA TCTCAAATGC TCCCATCCCG AAGAGCTCAT
    TCTATGTCGA CTTCTGGTTC TCCCAACTTG ACACCCGGGC CATCTATTCC ACTGCGACCT
    GCCTCCTCTC CTATTTTGTC CAACTCAAAC AAACCCCAGT CCAATACGTG GAGCAGTTCA
    AGTTGGCATG GAAGAGTTAA AGGTGGGATG AAAGGCTTCC AATCATTCAT GGTTTCGGAC
    AGCAACATGA ACTTTACTGA ATTTGTAGAG CTTTTCAAAT CTTTTAGCGT AAGAAGTCGG
    AAAGACCTGA AGGACATTTT TGATGTGTAT GCTGTGGCCT GCAATCGGTC AGGTGCAGAA
    TCAGTCCCTC TCTACACTAA TTTGACAATT GATGAGAATG TAGTTGGAGT CCAACCTGAT
    TTAGATTTAT TGACCAGGAA TGTGTCCGAC TTGGGTTTAT TCATTCGCAG CAGACAGCAA
    CTGTCTGAGA ACCAAAGGCA AATCTCAGAT GCCATTGCGG CTGCCAGCAT TGTAACTAAT
    GGAACTGGAG TAGAAAGCAC ATCCCTGGGC ATATTTGGAA TGGGAATACT GCAGTTTAAT
    GATTTCCTGG TGAATTGCCA GGGTGAACAT TACACCTATG ATGAAGTTCT TAGCATTATT
    CAGAAATTTG AACCAAGTAT CAGTATGCGC CAGCAGGGGC TGATGTCATT TGAAGGATTT
    GCAAGGTTCC TGATGGATAA AGACAATTTT GCATCAAGAA ATGATGAGTC TCAGGTAAAC
    ACAGAAGAAC TTCAGCTGCC CCTTTCCTAC TATTTCATTG AGTCTTCACA CAACACCTAC
    CTTACAGGGC ACCAGCTAAA AGGAGAGTCT TCAGTTGAGC TGTACAGCCA GGTCCTTTTG
    CAGGGGTGCC GAAGTGTTGA ATTAGACTGC TGGGATGGAG ATGACGGCAT GCCTATCATT
    TATCATGGAC ATACACTAAC TACTAAAATT CCCTTTAAGG ATGTCATTGA AGCTATTGGC
    CGAAGTGCAT TTATTACCTC TGAAATGCCA ATCGTACTGT CCATAGAAAA CCATTGCTCC
    CTACCACAGC AGCGTAAAAT GGCAGATATA TTTAAGAATG TCTTTGGTGA AAAACTGGTG
    ACAAAGTTCC TGTTTGAAAG TGACTTTTCA GATGATCCGA TGTTGCCTTC TCCATGGCAA
    TTGAGAAATA AAGTGCTTCT GAAAAATAAA AAGCTGAAGG CACATCAAAC CCCGGTGGAT
    ATACTGAAGC AGAAGGCACA TCAGCTGGCT AGCATGCAAG CACAAGCAAG CAATGGTAGC
    CAAATGGTAA GCCCTAGCAC AAACAATGAA GAAGAAGAAG ATGAAGAAGA TGAATATGAT
    TATGACTATG AGTCTCTGTC AGATGATAAT ATTCTTGATG ACCGATCTGA AACTAAAACC
    AACAGCGACA AACTGCAATT TGAATATAAT GAGGAAGCTT CAAAGCGAAT CAAAAAGACA
    GATGGCAGCA GTATTAACAC TAAAGGAAAG GTCTATGACA TGGAGTTGGG TGAGGAATTC
    TATCTTCCAC AAAACAAAAA AGAGAGCAGG CAAATTGCAC CTGAATTGTC AGACCTTATT
    ATCTATTGCC AAGCTGTCAA ATTCCCAGGC CTTACAACAT TAAACCCATG TGGATCTGGC
    AGAGGGAAGG AAAGAAAAAG CAGGAAGTCC ATTTTTGGCA ACAACCCTGG CAGAACAAGT
    CCTGGGGAAC CAACAGCACT TGCCAAAACA TCTGGAAAAG GTACAAATGA AACACGGCAG
    AGCTGGGAAG AGCCATGTTC TCCTCCCTTT AACCCCAGCA CTTCCCTCAG TGCAATAATC
    AGGACACCCA GGTGCTACCA TATCTCTTCC CTAAATGAGA ACGCAGCCAA GCGCTTGTGC
    CGAAGGTATT CTCAGAAACT CATTCAACAT ACCGCATATC AACTGCTCCG AACATATCCC
    GCCGCCACTC GGATAGACTC TTCCAACCCA CATCCTCTTA TTTTCTGGCT TCATGGTGTA
    CAGTTGGTTG CTCTAAACTA CCAAACTGAT GATCTCCCCT TACATCTAAA CGCCGCCATG
    TTTGAAGCAA ATGGTGGTTG CGGATATGTG TTAAAGCCCC CCGTGCAGTG GGATAGGACC
    TGCCCAATGT ACCAATTATT CTCACCATTA GAACGAGATT TAGAAAATAT GGAACCAGCA
    ATATATTCTT TGACGATAGT CTCTGGGCAA AATGTGTGTC CAGGTAACAG CTCAGGCAGC
    CCCTGCATTG AAATTGATGT CTTGGGTATG CCAGTAGACA GCTGCCATTT TCGGACCAAA
    CCAATCCATC GCAATACCTT AAATCCCATG TGGAATGAAC AATTCTTATT CCGAATATAC
    TTTGAGGATC TTGTGTTTCT TCGGTTTGCT GTGGTGGAAA ACAACAGCTC TGCTGTAACT
    GCCCAAAGAA TAATTAATCT TAAAGCTTTA AAGAGAGGTA ACAGACACCT TCAGCTAAGA
    AATTTACATA ATGAGCCCTT AGAGGTTTCT ACCTTATTTA TAAATAGCAG GAGAATGGAA
    GAAATCCCTA ATGGAAATAC CTTACCCGCC TCCTTGTTTT TCAGCTCAGA AGAAAGAAAG
    ACTCCAGCCA CATTTAAAGC TACAGTACAT GGGATTCCTG GGCCAGAACC ATTTACAGTG
    TTTGAAATCA GTCTTGGGAC AACTGCAAAG CAACTTCTTG ATCATATTTT GGCTACTATA
    CAAGGCAATG AAGCAGACAT CACTGACTAT TTTTTGATGG AGGAAAAATG CTTTATATCT
    AAAGACAAAA ATGAATGCAA GAAATTGCCG TTCCAGAGAG TGATTAGCCC AGAAGAGGAT
    ATCCTGCAAA TTTTGAATAG CTGGTTCCCA GAAGAAGGCT ACGTGGGGAG AATTGTTCTT
    AAAACACGAG AGGAGAACAT GAATGACAAA AATGTTCAAG AGGACAAAGA AGAAAAAGAG
    GTGACCGTGA GCTCCGATGA GGAGACTTTC TTTGTCCAGG TCCATGATGT TTCTCCTGAA
    CAGCCAAGAA CGGTTATCAA AGCTTTACGA TTCAGTACAG CGCAGGAAGT TATCCAGCAG
    GCTTTATGCA AGGCTAAATA CTCATACAGC ATCCTGAGCA ACCCTAATCC TAGTGATTAT
    GTGCTTCTGG AGGAAGTCAT CAAAGAAGCT TCAAATAAGA AGTCTTCTAC CTCCAAAACA
    ATTCAGAGGG TTCTCTTGGA TCAAGAGTGT GTATTCCAGG CACAGAGCAA GTGGAAGGGA
    GCAGGGAAAT TCATTCTTAA ACTGAAGGAA CAAGTCCAGG CGGCAAGAGA CGACAAGCGC
    AAAGGTCTGT CTATAGCCAG TGAACTGAAG AAGCTAGCAA AGTCCAGCCG GCAATCTCGG
    AACTTCACTT TGTCACCTCA GATATTTTTA TCAGAAGATG CCTGTGAAAG CCCTGAACCT
    ACAAAAAGCA AAACGCAGGA AAAGCCTTCG GCAGATAATG AAGTAAATGT GCATCCAACC
    TGCTGCAGGA GGTCTTCTGC TGCCTGGTAC TTTCTGTTGC TGAAGAACTG GAAGCTGCCA
    AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62382
    Clone ID Related Accession (Same CDS sequence) XM_031905948.1
    Accession Version XM_031905948.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCACTC CAAGAAGGTC
    TATGAGCCAA ACCGGGTTCT GTTTATTATG GGAGATTTAT CTGACTCTGA AAGTGATATC
    TTTGAACAAT CAAAAGAGTG GGACTTGCAT AGGAGGGAAG AGCAACAGAA GGCATTCAGC
    CATGGAACAG AGCTAATTCC CTGGTATGTG TTATCCATGA GAGCAGATGT CTATCAGTTT
    CTTCAGCAAG GAGTGACTGT CATCCGCTAT GACCAGGAGA CACACATTTC TGTTCGTTGT
    TTCCTTCAAC TCCAACCAGA CAACTCCACA TTGACTTGGA CAAAACCAAC CACAACGTGT
    CTTGCAAACA CTAAAAATAA ATTAGGAACT GTAAGTAGCA GTGCGGAGAT TAAATTTCAG
    TTCCTTGCCA ATGCTGGAAT GAATGGACTG GCCGAAGGAT TCTTGGACTT ATTCTCTGTA
    AAGGCAGTTT ACATGGGACA TCCGGGAATT GATATGCACA CTGTGTGTGT TCAAAACAAA
    CTATGTAATA TGAACCTGGA AGAAAATGGT GTCACATTGC TTTATGGGCT GCACACTACT
    GACAACAAGT TGCTGCACTT TGTAGCTCCG AAATACACAG CCAGGACATT GTATGATGGA
    CTCCTGGAGT TAACCAAAGC AGTAAGGAAG ATAAAACGCT TTCCTGATCA GAGATTACAG
    TGGTTAAGGA AGCAGTATGT TAGTCTCTAC CAGGAGGAAA ACAGATTTGA AGGACCAGCT
    TTGGCACAAG CTATTGAGCT GTTTGGAGGG CGACGCTGGA GTACGAGAAA CACGAGCACA
    GGGACCTTAA CCAAAAGCAC AGAGAAACCA AACGTGCAGA GGAATAACAC TCTCGGCATC
    AACACTGCCA AGAAGAAGAA GAAAGTGCTT ATGAGGGGCG AGAGTGGGGA TGCAGCCGAT
    GATGAGATGG CAACCCGCAA AACAAAAAGC TGCAAGGAAA GTCGCAGTAG AAGTGGCTCT
    GATCCTCCAG AAATCGATGA GCAGGAAGAG CAAGATCTGA ATATTATTGC TGGATATTCA
    CCATCTCAAA TGCTCCCATC CCGAAGAGCT CATTCTATGT CGACTTCTGG TTCTCCCAAC
    TTGACACCCG GGCCATCTAT TCCACTGCGA CCTGCCTCCT CTCCTATTTT GTCCAACTCA
    AACAAACCCC AGTCCAATAC GTGGAGCAGT TCAAGTTGGC ATGGAAGAGT TAAAGGTGGG
    ATGAAAGGCT TCCAATCATT CATGGTTTCG GACAGCAACA TGAACTTTAC TGAATTTGTA
    GAGCTTTTCA AATCTTTTAG CGTAAGAAGT CGGAAAGACC TGAAGGACAT TTTTGATGTG
    TATGCTGTGG CCTGCAATCG GTCAGGTGCA GAATCAGTCC CTCTCTACAC TAATTTGACA
    ATTGATGAGA ATGTAGTTGG AGTCCAACCT GATTTAGATT TATTGACCAG GAATGTGTCC
    GACTTGGGTT TATTCATTCG CAGCAGACAG CAACTGTCTG AGAACCAAAG GCAAATCTCA
    GATGCCATTG CGGCTGCCAG CATTGTAACT AATGGAACTG GAGTAGAAAG CACATCCCTG
    GGCATATTTG GAATGGGAAT ACTGCAGTTT AATGATTTCC TGGTGAATTG CCAGGGTGAA
    CATTACACCT ATGATGAAGT TCTTAGCATT ATTCAGAAAT TTGAACCAAG TATCAGTATG
    CGCCAGCAGG GGCTGATGTC ATTTGAAGGA TTTGCAAGGT TCCTGATGGA TAAAGACAAT
    TTTGCATCAA GAAATGATGA GTCTCAGGTA AACACAGAAG AACTTCAGCT GCCCCTTTCC
    TACTATTTCA TTGAGTCTTC ACACAACACC TACCTTACAG GGCACCAGCT AAAAGGAGAG
    TCTTCAGTTG AGCTGTACAG CCAGGTCCTT TTGCAGGGGT GCCGAAGTGT TGAATTAGAC
    TGCTGGGATG GAGATGACGG CATGCCTATC ATTTATCATG GACATACACT AACTACTAAA
    ATTCCCTTTA AGGATGTCAT TGAAGCTATT GGCCGAAGTG CATTTATTAC CTCTGAAATG
    CCAATCGTAC TGTCCATAGA AAACCATTGC TCCCTACCAC AGCAGCGTAA AATGGCAGAT
    ATATTTAAGA ATGTCTTTGG TGAAAAACTG GTGACAAAGT TCCTGTTTGA AAGTGACTTT
    TCAGATGATC CGATGTTGCC TTCTCCATGG CAATTGAGAA ATAAAGTGCT TCTGAAAAAT
    AAAAAGCTGA AGGCACATCA AACCCCGGTG GATATACTGA AGCAGAAGGC ACATCAGCTG
    GCTAGCATGC AAGCACAAGC AAGCAATGGT AGCCAAATGG TAAGCCCTAG CACAAACAAT
    GAAGAAGAAG AAGATGAAGA AGATGAATAT GATTATGACT ATGAGTCTCT GTCAGATGCC
    GATGTCCTTA ATGCTTCCCC TGCTCCTAAC AGCCAGGAAG ATAATATTCT TGATGACCGA
    TCTGAAACTA AAACCAACAG CGACAAACTG CAATTTGAAT ATAATGAGGA AGCTTCAAAG
    CGAATCAAAA AGACAGATGG CAGCAGTATT AACACTAAAG GAAAGGTCTA TGACATGGAG
    TTGGGTGAGG AATTCTATCT TCCACAAAAC AAAAAAGAGA GCAGGCAAAT TGCACCTGAA
    TTGTCAGACC TTATTATCTA TTGCCAAGCT GTCAAATTCC CAGGCCTTAC AACATTAAAC
    CCATGTGGAT CTGGCAGAGG GAAGGAAAGA AAAAGCAGGA AGTCCATTTT TGGCAACAAC
    CCTGGCAGAA CAAGTCCTGG GGAACCAACA GCACTTGCCA AAACATCTGG AAAAGGTACA
    AATGAAACAC GGCAGAGCTG GGAAGAGCCA TGTTCTCCTC CCTTTAACCC CAGCACTTCC
    CTCAGTGCAA TAATCAGGAC ACCCAGGTGC TACCATATCT CTTCCCTAAA TGAGAACGCA
    GCCAAGCGCT TGTGCCGAAG GTATTCTCAG AAACTCATTC AACATACCGC ATATCAACTG
    CTCCGAACAT ATCCCGCCGC CACTCGGATA GACTCTTCCA ACCCACATCC TCTTATTTTC
    TGGCTTCATG GTGTACAGTT GGTTGCTCTA AACTACCAAA CTGATGATCT CCCCTTACAT
    CTAAACGCCG CCATGTTTGA AGCAAATGGT GGTTGCGGAT ATGTGTTAAA GCCCCCCGTG
    CAGTGGGATA GGACCTGCCC AATGTACCAA TTATTCTCAC CATTAGAACG AGATTTAGAA
    AATATGGAAC CAGCAATATA TTCTTTGACG ATAGTCTCTG GGCAAAATGT GTGTCCAGGT
    AACAGCTCAG GCAGCCCCTG CATTGAAATT GATGTCTTGG GTATGCCAGT AGACAGCTGC
    CATTTTCGGA CCAAACCAAT CCATCGCAAT ACCTTAAATC CCATGTGGAA TGAACAATTC
    TTATTCCGAA TATACTTTGA GGATCTTGTG TTTCTTCGGT TTGCTGTGGT GGAAAACAAC
    AGCTCTGCTG TAACTGCCCA AAGAATAATT AATCTTAAAG CTTTAAAGAG AGGTAACAGA
    CACCTTCAGC TAAGAAATTT ACATAATGAG CCCTTAGAGG TTTCTACCTT ATTTATAAAT
    AGCAGGAGAA TGGAAGAAAT CCCTAATGGA AATACCTTAC CCGCCTCCTT GTTTTTCAGC
    TCAGAAGAAA GAAAGACTCC AGCCACATTT AAAGCTACAG TACATGGGAT TCCTGGGCCA
    GAACCATTTA CAGTGTTTGA AATCAGTCTT GGGACAACTG CAAAGCAACT TCTTGATCAT
    ATTTTGGCTA CTATACAAGG CAATGAAGCA GACATCACTG ACTATTTTTT GATGGAGGAA
    AAATGCTTTA TATCTAAAGA CAAAAATGAA TGCAAGAAAT TGCCGTTCCA GAGAGTGATT
    AGCCCAGAAG AGGATATCCT GCAAATTTTG AATAGCTGGT TCCCAGAAGA AGGCTACGTG
    GGGAGAATTG TTCTTAAAAC ACGAGAGGAG AACATGAATG ACAAAAATGT TCAAGAGGAC
    AAAGAAGAAA AAGAGGTGAC CGTGAGCTCC GATGAGGAGA CTTTCTTTGT CCAGGTCCAT
    GATGTTTCTC CTGAACAGCC AAGAACGGTT ATCAAAGCTT TACGATTCAG TACAGCGCAG
    GAAGTTATCC AGCAGGCTTT ATGCAAGGCT AAATACTCAT ACAGCATCCT GAGCAACCCT
    AATCCTAGTG ATTATGTGCT TCTGGAGGAA GTCATCAAAG AAGCTTCAAA TAAGAAGTCT
    TCTACCTCCA AAACAATTCA GAGGGTTCTC TTGGATCAAG AGTGTGTATT CCAGGCACAG
    AGCAAGTGGA AGGGAGCAGG GAAATTCATT CTTAAACTGA AGGAACAAGT CCAGGCGGCA
    AGAGACGACA AGCGCAAAGG TCTGTCTATA GCCAGTGAAC TGAAGAAGCT AGCAAAGTCC
    AGCCGGCAAT CTCGGAACTT CACTTTGTCA CCTCAGATAT TTTTATCAGA AGGTACCCAG
    AATAGGGATG AAAAACCTGC ATGCAGTTTG TCCTTCAGTG ACATCAATGA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761808.1
    CDS567..7520
    Translation

    Target ORF information:

    RefSeq Version XM_031905948.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905948.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCACTC CAAGAAGGTC
    TATGAGCCAA ACCGGGTTCT GTTTATTATG GGAGATTTAT CTGACTCTGA AAGTGATATC
    TTTGAACAAT CAAAAGAGTG GGACTTGCAT AGGAGGGAAG AGCAACAGAA GGCATTCAGC
    CATGGAACAG AGCTAATTCC CTGGTATGTG TTATCCATGA GAGCAGATGT CTATCAGTTT
    CTTCAGCAAG GAGTGACTGT CATCCGCTAT GACCAGGAGA CACACATTTC TGTTCGTTGT
    TTCCTTCAAC TCCAACCAGA CAACTCCACA TTGACTTGGA CAAAACCAAC CACAACGTGT
    CTTGCAAACA CTAAAAATAA ATTAGGAACT GTAAGTAGCA GTGCGGAGAT TAAATTTCAG
    TTCCTTGCCA ATGCTGGAAT GAATGGACTG GCCGAAGGAT TCTTGGACTT ATTCTCTGTA
    AAGGCAGTTT ACATGGGACA TCCGGGAATT GATATGCACA CTGTGTGTGT TCAAAACAAA
    CTATGTAATA TGAACCTGGA AGAAAATGGT GTCACATTGC TTTATGGGCT GCACACTACT
    GACAACAAGT TGCTGCACTT TGTAGCTCCG AAATACACAG CCAGGACATT GTATGATGGA
    CTCCTGGAGT TAACCAAAGC AGTAAGGAAG ATAAAACGCT TTCCTGATCA GAGATTACAG
    TGGTTAAGGA AGCAGTATGT TAGTCTCTAC CAGGAGGAAA ACAGATTTGA AGGACCAGCT
    TTGGCACAAG CTATTGAGCT GTTTGGAGGG CGACGCTGGA GTACGAGAAA CACGAGCACA
    GGGACCTTAA CCAAAAGCAC AGAGAAACCA AACGTGCAGA GGAATAACAC TCTCGGCATC
    AACACTGCCA AGAAGAAGAA GAAAGTGCTT ATGAGGGGCG AGAGTGGGGA TGCAGCCGAT
    GATGAGATGG CAACCCGCAA AACAAAAAGC TGCAAGGAAA GTCGCAGTAG AAGTGGCTCT
    GATCCTCCAG AAATCGATGA GCAGGAAGAG CAAGATCTGA ATATTATTGC TGGATATTCA
    CCATCTCAAA TGCTCCCATC CCGAAGAGCT CATTCTATGT CGACTTCTGG TTCTCCCAAC
    TTGACACCCG GGCCATCTAT TCCACTGCGA CCTGCCTCCT CTCCTATTTT GTCCAACTCA
    AACAAACCCC AGTCCAATAC GTGGAGCAGT TCAAGTTGGC ATGGAAGAGT TAAAGGTGGG
    ATGAAAGGCT TCCAATCATT CATGGTTTCG GACAGCAACA TGAACTTTAC TGAATTTGTA
    GAGCTTTTCA AATCTTTTAG CGTAAGAAGT CGGAAAGACC TGAAGGACAT TTTTGATGTG
    TATGCTGTGG CCTGCAATCG GTCAGGTGCA GAATCAGTCC CTCTCTACAC TAATTTGACA
    ATTGATGAGA ATGTAGTTGG AGTCCAACCT GATTTAGATT TATTGACCAG GAATGTGTCC
    GACTTGGGTT TATTCATTCG CAGCAGACAG CAACTGTCTG AGAACCAAAG GCAAATCTCA
    GATGCCATTG CGGCTGCCAG CATTGTAACT AATGGAACTG GAGTAGAAAG CACATCCCTG
    GGCATATTTG GAATGGGAAT ACTGCAGTTT AATGATTTCC TGGTGAATTG CCAGGGTGAA
    CATTACACCT ATGATGAAGT TCTTAGCATT ATTCAGAAAT TTGAACCAAG TATCAGTATG
    CGCCAGCAGG GGCTGATGTC ATTTGAAGGA TTTGCAAGGT TCCTGATGGA TAAAGACAAT
    TTTGCATCAA GAAATGATGA GTCTCAGGTA AACACAGAAG AACTTCAGCT GCCCCTTTCC
    TACTATTTCA TTGAGTCTTC ACACAACACC TACCTTACAG GGCACCAGCT AAAAGGAGAG
    TCTTCAGTTG AGCTGTACAG CCAGGTCCTT TTGCAGGGGT GCCGAAGTGT TGAATTAGAC
    TGCTGGGATG GAGATGACGG CATGCCTATC ATTTATCATG GACATACACT AACTACTAAA
    ATTCCCTTTA AGGATGTCAT TGAAGCTATT GGCCGAAGTG CATTTATTAC CTCTGAAATG
    CCAATCGTAC TGTCCATAGA AAACCATTGC TCCCTACCAC AGCAGCGTAA AATGGCAGAT
    ATATTTAAGA ATGTCTTTGG TGAAAAACTG GTGACAAAGT TCCTGTTTGA AAGTGACTTT
    TCAGATGATC CGATGTTGCC TTCTCCATGG CAATTGAGAA ATAAAGTGCT TCTGAAAAAT
    AAAAAGCTGA AGGCACATCA AACCCCGGTG GATATACTGA AGCAGAAGGC ACATCAGCTG
    GCTAGCATGC AAGCACAAGC AAGCAATGGT AGCCAAATGG TAAGCCCTAG CACAAACAAT
    GAAGAAGAAG AAGATGAAGA AGATGAATAT GATTATGACT ATGAGTCTCT GTCAGATGCC
    GATGTCCTTA ATGCTTCCCC TGCTCCTAAC AGCCAGGAAG ATAATATTCT TGATGACCGA
    TCTGAAACTA AAACCAACAG CGACAAACTG CAATTTGAAT ATAATGAGGA AGCTTCAAAG
    CGAATCAAAA AGACAGATGG CAGCAGTATT AACACTAAAG GAAAGGTCTA TGACATGGAG
    TTGGGTGAGG AATTCTATCT TCCACAAAAC AAAAAAGAGA GCAGGCAAAT TGCACCTGAA
    TTGTCAGACC TTATTATCTA TTGCCAAGCT GTCAAATTCC CAGGCCTTAC AACATTAAAC
    CCATGTGGAT CTGGCAGAGG GAAGGAAAGA AAAAGCAGGA AGTCCATTTT TGGCAACAAC
    CCTGGCAGAA CAAGTCCTGG GGAACCAACA GCACTTGCCA AAACATCTGG AAAAGGTACA
    AATGAAACAC GGCAGAGCTG GGAAGAGCCA TGTTCTCCTC CCTTTAACCC CAGCACTTCC
    CTCAGTGCAA TAATCAGGAC ACCCAGGTGC TACCATATCT CTTCCCTAAA TGAGAACGCA
    GCCAAGCGCT TGTGCCGAAG GTATTCTCAG AAACTCATTC AACATACCGC ATATCAACTG
    CTCCGAACAT ATCCCGCCGC CACTCGGATA GACTCTTCCA ACCCACATCC TCTTATTTTC
    TGGCTTCATG GTGTACAGTT GGTTGCTCTA AACTACCAAA CTGATGATCT CCCCTTACAT
    CTAAACGCCG CCATGTTTGA AGCAAATGGT GGTTGCGGAT ATGTGTTAAA GCCCCCCGTG
    CAGTGGGATA GGACCTGCCC AATGTACCAA TTATTCTCAC CATTAGAACG AGATTTAGAA
    AATATGGAAC CAGCAATATA TTCTTTGACG ATAGTCTCTG GGCAAAATGT GTGTCCAGGT
    AACAGCTCAG GCAGCCCCTG CATTGAAATT GATGTCTTGG GTATGCCAGT AGACAGCTGC
    CATTTTCGGA CCAAACCAAT CCATCGCAAT ACCTTAAATC CCATGTGGAA TGAACAATTC
    TTATTCCGAA TATACTTTGA GGATCTTGTG TTTCTTCGGT TTGCTGTGGT GGAAAACAAC
    AGCTCTGCTG TAACTGCCCA AAGAATAATT AATCTTAAAG CTTTAAAGAG AGGTAACAGA
    CACCTTCAGC TAAGAAATTT ACATAATGAG CCCTTAGAGG TTTCTACCTT ATTTATAAAT
    AGCAGGAGAA TGGAAGAAAT CCCTAATGGA AATACCTTAC CCGCCTCCTT GTTTTTCAGC
    TCAGAAGAAA GAAAGACTCC AGCCACATTT AAAGCTACAG TACATGGGAT TCCTGGGCCA
    GAACCATTTA CAGTGTTTGA AATCAGTCTT GGGACAACTG CAAAGCAACT TCTTGATCAT
    ATTTTGGCTA CTATACAAGG CAATGAAGCA GACATCACTG ACTATTTTTT GATGGAGGAA
    AAATGCTTTA TATCTAAAGA CAAAAATGAA TGCAAGAAAT TGCCGTTCCA GAGAGTGATT
    AGCCCAGAAG AGGATATCCT GCAAATTTTG AATAGCTGGT TCCCAGAAGA AGGCTACGTG
    GGGAGAATTG TTCTTAAAAC ACGAGAGGAG AACATGAATG ACAAAAATGT TCAAGAGGAC
    AAAGAAGAAA AAGAGGTGAC CGTGAGCTCC GATGAGGAGA CTTTCTTTGT CCAGGTCCAT
    GATGTTTCTC CTGAACAGCC AAGAACGGTT ATCAAAGCTT TACGATTCAG TACAGCGCAG
    GAAGTTATCC AGCAGGCTTT ATGCAAGGCT AAATACTCAT ACAGCATCCT GAGCAACCCT
    AATCCTAGTG ATTATGTGCT TCTGGAGGAA GTCATCAAAG AAGCTTCAAA TAAGAAGTCT
    TCTACCTCCA AAACAATTCA GAGGGTTCTC TTGGATCAAG AGTGTGTATT CCAGGCACAG
    AGCAAGTGGA AGGGAGCAGG GAAATTCATT CTTAAACTGA AGGAACAAGT CCAGGCGGCA
    AGAGACGACA AGCGCAAAGG TCTGTCTATA GCCAGTGAAC TGAAGAAGCT AGCAAAGTCC
    AGCCGGCAAT CTCGGAACTT CACTTTGTCA CCTCAGATAT TTTTATCAGA AGGTACCCAG
    AATAGGGATG AAAAACCTGC ATGCAGTTTG TCCTTCAGTG ACATCAATGA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62383
    Clone ID Related Accession (Same CDS sequence) XM_031905949.1
    Accession Version XM_031905949.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCACTC CAAGAAGGTC
    TATGAGCCAA ACCGGGTTCT GTTTATTATG GGAGATTTAT CTGACTCTGA AAGTGATATC
    TTTGAACAAT CAAAAGAGTG GGACTTGCAT AGGAGGGAAG AGCAACAGAA GGCATTCAGC
    CATGGAACAG AGCTAATTCC CTGGTATGTG TTATCCATGA GAGCAGATGT CTATCAGTTT
    CTTCAGCAAG GAGTGACTGT CATCCGCTAT GACCAGGAGA CACACATTTC TGTTCGTTGT
    TTCCTTCAAC TCCAACCAGA CAACTCCACA TTGACTTGGA CAAAACCAAC CACAACGTGT
    CTTGCAAACA CTAAAAATAA ATTAGGAACT GTAAGTAGCA GTGCGGAGAT TAAATTTCAG
    TTCCTTGCCA ATGCTGGAAT GAATGGACTG GCCGAAGGAT TCTTGGACTT ATTCTCTGTA
    AAGGCAGTTT ACATGGGACA TCCGGGAATT GATATGCACA CTGTGTGTGT TCAAAACAAA
    CTATGTAATA TGAACCTGGA AGAAAATGGT GTCACATTGC TTTATGGGCT GCACACTACT
    GACAACAAGT TGCTGCACTT TGTAGCTCCG AAATACACAG CCAGGACATT GTATGATGGA
    CTCCTGGAGT TAACCAAAGC AGTAAGGAAG ATAAAACGCT TTCCTGATCA GAGATTACAG
    TGGTTAAGGA AGCAGTATGT TAGTCTCTAC CAGGAGGAAA ACAGATTTGA AGGACCAGCT
    TTGGCACAAG CTATTGAGCT GTTTGGAGGG CGACGCTGGA GTACGAGAAA CACGAGCACA
    GGGACCTTAA CCAAAAGCAC AGAGAAACCA AACGTGCAGA GGAATAACAC TCTCGGCATC
    AACACTGCCA AGAAGAAGAA GAAAGTGCTT ATGAGGGGCG AGAGTGGGGA TGCAGCCGAT
    GATGAGATGG CAACCCGCAA AACAAAAAGC TGCAAGGAAA GTCGCAGTAG AAGTGGCTCT
    GATCCTCCAG AAATCGATGA GCAGGAAGAG CAAGATCTGA ATATTATTGC TGGATATTCA
    CCATCTCAAA TGCTCCCATC CCGAAGAGCT CATTCTATGT CGACTTCTGG TTCTCCCAAC
    TTGACACCCG GGCCATCTAT TCCACTGCGA CCTGCCTCCT CTCCTATTTT GTCCAACTCA
    AACAAACCCC AGTCCAATAC GTGGAGCAGT TCAAGTTGGC ATGGAAGAGT TAAAGGTGGG
    ATGAAAGGCT TCCAATCATT CATGGTTTCG GACAGCAACA TGAACTTTAC TGAATTTGTA
    GAGCTTTTCA AATCTTTTAG CGTAAGAAGT CGGAAAGACC TGAAGGACAT TTTTGATGTG
    TATGCTGTGG CCTGCAATCG GTCAGGTGCA GAATCAGTCC CTCTCTACAC TAATTTGACA
    ATTGATGAGA ATGTAGTTGG AGTCCAACCT GATTTAGATT TATTGACCAG GAATGTGTCC
    GACTTGGGTT TATTCATTCG CAGCAGACAG CAACTGTCTG AGAACCAAAG GCAAATCTCA
    GATGCCATTG CGGCTGCCAG CATTGTAACT AATGGAACTG GAGTAGAAAG CACATCCCTG
    GGCATATTTG GAATGGGAAT ACTGCAGTTT AATGATTTCC TGGTGAATTG CCAGGGTGAA
    CATTACACCT ATGATGAAGT TCTTAGCATT ATTCAGAAAT TTGAACCAAG TATCAGTATG
    CGCCAGCAGG GGCTGATGTC ATTTGAAGGA TTTGCAAGGT TCCTGATGGA TAAAGACAAT
    TTTGCATCAA GAAATGATGA GTCTCAGGTA AACACAGAAG AACTTCAGCT GCCCCTTTCC
    TACTATTTCA TTGAGTCTTC ACACAACACC TACCTTACAG GGCACCAGCT AAAAGGAGAG
    TCTTCAGTTG AGCTGTACAG CCAGGTCCTT TTGCAGGGGT GCCGAAGTGT TGAATTAGAC
    TGCTGGGATG GAGATGACGG CATGCCTATC ATTTATCATG GACATACACT AACTACTAAA
    ATTCCCTTTA AGGATGTCAT TGAAGCTATT GGCCGAAGTG CATTTATTAC CTCTGAAATG
    CCAATCGTAC TGTCCATAGA AAACCATTGC TCCCTACCAC AGCAGCGTAA AATGGCAGAT
    ATATTTAAGA ATGTCTTTGG TGAAAAACTG GTGACAAAGT TCCTGTTTGA AAGTGACTTT
    TCAGATGATC CGATGTTGCC TTCTCCATGG CAATTGAGAA ATAAAGTGCT TCTGAAAAAT
    AAAAAGCTGA AGGCACATCA AACCCCGGTG GATATACTGA AGCAGAAGGC ACATCAGCTG
    GCTAGCATGC AAGCACAAGC AAGCAATGGT AGCCAAATGG TAAGCCCTAG CACAAACAAT
    GAAGAAGAAG AAGATGAAGA AGATGAATAT GATTATGACT ATGAGTCTCT GTCAGATGAT
    AATATTCTTG ATGACCGATC TGAAACTAAA ACCAACAGCG ACAAACTGCA ATTTGAATAT
    AATGAGGAAG CTTCAAAGCG AATCAAAAAG ACAGATGGCA GCAGTATTAA CACTAAAGGA
    AAGGTCTATG ACATGGAGTT GGGTGAGGAA TTCTATCTTC CACAAAACAA AAAAGAGAGC
    AGGCAAATTG CACCTGAATT GTCAGACCTT ATTATCTATT GCCAAGCTGT CAAATTCCCA
    GGCCTTACAA CATTAAACCC ATGTGGATCT GGCAGAGGGA AGGAAAGAAA AAGCAGGAAG
    TCCATTTTTG GCAACAACCC TGGCAGAACA AGTCCTGGGG AACCAACAGC ACTTGCCAAA
    ACATCTGGAA AAGGTACAAA TGAAACACGG CAGAGCTGGG AAGAGCCATG TTCTCCTCCC
    TTTAACCCCA GCACTTCCCT CAGTGCAATA ATCAGGACAC CCAGGTGCTA CCATATCTCT
    TCCCTAAATG AGAACGCAGC CAAGCGCTTG TGCCGAAGGT ATTCTCAGAA ACTCATTCAA
    CATACCGCAT ATCAACTGCT CCGAACATAT CCCGCCGCCA CTCGGATAGA CTCTTCCAAC
    CCACATCCTC TTATTTTCTG GCTTCATGGT GTACAGTTGG TTGCTCTAAA CTACCAAACT
    GATGATCTCC CCTTACATCT AAACGCCGCC ATGTTTGAAG CAAATGGTGG TTGCGGATAT
    GTGTTAAAGC CCCCCGTGCA GTGGGATAGG ACCTGCCCAA TGTACCAATT ATTCTCACCA
    TTAGAACGAG ATTTAGAAAA TATGGAACCA GCAATATATT CTTTGACGAT AGTCTCTGGG
    CAAAATGTGT GTCCAGGTAA CAGCTCAGGC AGCCCCTGCA TTGAAATTGA TGTCTTGGGT
    ATGCCAGTAG ACAGCTGCCA TTTTCGGACC AAACCAATCC ATCGCAATAC CTTAAATCCC
    ATGTGGAATG AACAATTCTT ATTCCGAATA TACTTTGAGG ATCTTGTGTT TCTTCGGTTT
    GCTGTGGTGG AAAACAACAG CTCTGCTGTA ACTGCCCAAA GAATAATTAA TCTTAAAGCT
    TTAAAGAGAG GTAACAGACA CCTTCAGCTA AGAAATTTAC ATAATGAGCC CTTAGAGGTT
    TCTACCTTAT TTATAAATAG CAGGAGAATG GAAGAAATCC CTAATGGAAA TACCTTACCC
    GCCTCCTTGT TTTTCAGCTC AGAAGAAAGA AAGACTCCAG CCACATTTAA AGCTACAGTA
    CATGGGATTC CTGGGCCAGA ACCATTTACA GTGTTTGAAA TCAGTCTTGG GACAACTGCA
    AAGCAACTTC TTGATCATAT TTTGGCTACT ATACAAGGCA ATGAAGCAGA CATCACTGAC
    TATTTTTTGA TGGAGGAAAA ATGCTTTATA TCTAAAGACA AAAATGAATG CAAGAAATTG
    CCGTTCCAGA GAGTGATTAG CCCAGAAGAG GATATCCTGC AAATTTTGAA TAGCTGGTTC
    CCAGAAGAAG GCTACGTGGG GAGAATTGTT CTTAAAACAC GAGAGGAGAA CATGAATGAC
    AAAAATGTTC AAGAGGACAA AGAAGAAAAA GAGGTGACCG TGAGCTCCGA TGAGGAGACT
    TTCTTTGTCC AGGTCCATGA TGTTTCTCCT GAACAGCCAA GAACGGTTAT CAAAGCTTTA
    CGATTCAGTA CAGCGCAGGA AGTTATCCAG CAGGCTTTAT GCAAGGCTAA ATACTCATAC
    AGCATCCTGA GCAACCCTAA TCCTAGTGAT TATGTGCTTC TGGAGGAAGT CATCAAAGAA
    GCTTCAAATA AGAAGTCTTC TACCTCCAAA ACAATTCAGA GGGTTCTCTT GGATCAAGAG
    TGTGTATTCC AGGCACAGAG CAAGTGGAAG GGAGCAGGGA AATTCATTCT TAAACTGAAG
    GAACAAGTCC AGGCGGCAAG AGACGACAAG CGCAAAGGTC TGTCTATAGC CAGTGAACTG
    AAGAAGCTAG CAAAGTCCAG CCGGCAATCT CGGAACTTCA CTTTGTCACC TCAGATATTT
    TTATCAGAAG GTACCCAGAA TAGGGATGAA AAACCTGCAT GCAGTTTGTC CTTCAGTGAC
    ATCAATGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761809.1
    CDS566..7477
    Translation

    Target ORF information:

    RefSeq Version XM_031905949.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905949.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCACTC CAAGAAGGTC
    TATGAGCCAA ACCGGGTTCT GTTTATTATG GGAGATTTAT CTGACTCTGA AAGTGATATC
    TTTGAACAAT CAAAAGAGTG GGACTTGCAT AGGAGGGAAG AGCAACAGAA GGCATTCAGC
    CATGGAACAG AGCTAATTCC CTGGTATGTG TTATCCATGA GAGCAGATGT CTATCAGTTT
    CTTCAGCAAG GAGTGACTGT CATCCGCTAT GACCAGGAGA CACACATTTC TGTTCGTTGT
    TTCCTTCAAC TCCAACCAGA CAACTCCACA TTGACTTGGA CAAAACCAAC CACAACGTGT
    CTTGCAAACA CTAAAAATAA ATTAGGAACT GTAAGTAGCA GTGCGGAGAT TAAATTTCAG
    TTCCTTGCCA ATGCTGGAAT GAATGGACTG GCCGAAGGAT TCTTGGACTT ATTCTCTGTA
    AAGGCAGTTT ACATGGGACA TCCGGGAATT GATATGCACA CTGTGTGTGT TCAAAACAAA
    CTATGTAATA TGAACCTGGA AGAAAATGGT GTCACATTGC TTTATGGGCT GCACACTACT
    GACAACAAGT TGCTGCACTT TGTAGCTCCG AAATACACAG CCAGGACATT GTATGATGGA
    CTCCTGGAGT TAACCAAAGC AGTAAGGAAG ATAAAACGCT TTCCTGATCA GAGATTACAG
    TGGTTAAGGA AGCAGTATGT TAGTCTCTAC CAGGAGGAAA ACAGATTTGA AGGACCAGCT
    TTGGCACAAG CTATTGAGCT GTTTGGAGGG CGACGCTGGA GTACGAGAAA CACGAGCACA
    GGGACCTTAA CCAAAAGCAC AGAGAAACCA AACGTGCAGA GGAATAACAC TCTCGGCATC
    AACACTGCCA AGAAGAAGAA GAAAGTGCTT ATGAGGGGCG AGAGTGGGGA TGCAGCCGAT
    GATGAGATGG CAACCCGCAA AACAAAAAGC TGCAAGGAAA GTCGCAGTAG AAGTGGCTCT
    GATCCTCCAG AAATCGATGA GCAGGAAGAG CAAGATCTGA ATATTATTGC TGGATATTCA
    CCATCTCAAA TGCTCCCATC CCGAAGAGCT CATTCTATGT CGACTTCTGG TTCTCCCAAC
    TTGACACCCG GGCCATCTAT TCCACTGCGA CCTGCCTCCT CTCCTATTTT GTCCAACTCA
    AACAAACCCC AGTCCAATAC GTGGAGCAGT TCAAGTTGGC ATGGAAGAGT TAAAGGTGGG
    ATGAAAGGCT TCCAATCATT CATGGTTTCG GACAGCAACA TGAACTTTAC TGAATTTGTA
    GAGCTTTTCA AATCTTTTAG CGTAAGAAGT CGGAAAGACC TGAAGGACAT TTTTGATGTG
    TATGCTGTGG CCTGCAATCG GTCAGGTGCA GAATCAGTCC CTCTCTACAC TAATTTGACA
    ATTGATGAGA ATGTAGTTGG AGTCCAACCT GATTTAGATT TATTGACCAG GAATGTGTCC
    GACTTGGGTT TATTCATTCG CAGCAGACAG CAACTGTCTG AGAACCAAAG GCAAATCTCA
    GATGCCATTG CGGCTGCCAG CATTGTAACT AATGGAACTG GAGTAGAAAG CACATCCCTG
    GGCATATTTG GAATGGGAAT ACTGCAGTTT AATGATTTCC TGGTGAATTG CCAGGGTGAA
    CATTACACCT ATGATGAAGT TCTTAGCATT ATTCAGAAAT TTGAACCAAG TATCAGTATG
    CGCCAGCAGG GGCTGATGTC ATTTGAAGGA TTTGCAAGGT TCCTGATGGA TAAAGACAAT
    TTTGCATCAA GAAATGATGA GTCTCAGGTA AACACAGAAG AACTTCAGCT GCCCCTTTCC
    TACTATTTCA TTGAGTCTTC ACACAACACC TACCTTACAG GGCACCAGCT AAAAGGAGAG
    TCTTCAGTTG AGCTGTACAG CCAGGTCCTT TTGCAGGGGT GCCGAAGTGT TGAATTAGAC
    TGCTGGGATG GAGATGACGG CATGCCTATC ATTTATCATG GACATACACT AACTACTAAA
    ATTCCCTTTA AGGATGTCAT TGAAGCTATT GGCCGAAGTG CATTTATTAC CTCTGAAATG
    CCAATCGTAC TGTCCATAGA AAACCATTGC TCCCTACCAC AGCAGCGTAA AATGGCAGAT
    ATATTTAAGA ATGTCTTTGG TGAAAAACTG GTGACAAAGT TCCTGTTTGA AAGTGACTTT
    TCAGATGATC CGATGTTGCC TTCTCCATGG CAATTGAGAA ATAAAGTGCT TCTGAAAAAT
    AAAAAGCTGA AGGCACATCA AACCCCGGTG GATATACTGA AGCAGAAGGC ACATCAGCTG
    GCTAGCATGC AAGCACAAGC AAGCAATGGT AGCCAAATGG TAAGCCCTAG CACAAACAAT
    GAAGAAGAAG AAGATGAAGA AGATGAATAT GATTATGACT ATGAGTCTCT GTCAGATGAT
    AATATTCTTG ATGACCGATC TGAAACTAAA ACCAACAGCG ACAAACTGCA ATTTGAATAT
    AATGAGGAAG CTTCAAAGCG AATCAAAAAG ACAGATGGCA GCAGTATTAA CACTAAAGGA
    AAGGTCTATG ACATGGAGTT GGGTGAGGAA TTCTATCTTC CACAAAACAA AAAAGAGAGC
    AGGCAAATTG CACCTGAATT GTCAGACCTT ATTATCTATT GCCAAGCTGT CAAATTCCCA
    GGCCTTACAA CATTAAACCC ATGTGGATCT GGCAGAGGGA AGGAAAGAAA AAGCAGGAAG
    TCCATTTTTG GCAACAACCC TGGCAGAACA AGTCCTGGGG AACCAACAGC ACTTGCCAAA
    ACATCTGGAA AAGGTACAAA TGAAACACGG CAGAGCTGGG AAGAGCCATG TTCTCCTCCC
    TTTAACCCCA GCACTTCCCT CAGTGCAATA ATCAGGACAC CCAGGTGCTA CCATATCTCT
    TCCCTAAATG AGAACGCAGC CAAGCGCTTG TGCCGAAGGT ATTCTCAGAA ACTCATTCAA
    CATACCGCAT ATCAACTGCT CCGAACATAT CCCGCCGCCA CTCGGATAGA CTCTTCCAAC
    CCACATCCTC TTATTTTCTG GCTTCATGGT GTACAGTTGG TTGCTCTAAA CTACCAAACT
    GATGATCTCC CCTTACATCT AAACGCCGCC ATGTTTGAAG CAAATGGTGG TTGCGGATAT
    GTGTTAAAGC CCCCCGTGCA GTGGGATAGG ACCTGCCCAA TGTACCAATT ATTCTCACCA
    TTAGAACGAG ATTTAGAAAA TATGGAACCA GCAATATATT CTTTGACGAT AGTCTCTGGG
    CAAAATGTGT GTCCAGGTAA CAGCTCAGGC AGCCCCTGCA TTGAAATTGA TGTCTTGGGT
    ATGCCAGTAG ACAGCTGCCA TTTTCGGACC AAACCAATCC ATCGCAATAC CTTAAATCCC
    ATGTGGAATG AACAATTCTT ATTCCGAATA TACTTTGAGG ATCTTGTGTT TCTTCGGTTT
    GCTGTGGTGG AAAACAACAG CTCTGCTGTA ACTGCCCAAA GAATAATTAA TCTTAAAGCT
    TTAAAGAGAG GTAACAGACA CCTTCAGCTA AGAAATTTAC ATAATGAGCC CTTAGAGGTT
    TCTACCTTAT TTATAAATAG CAGGAGAATG GAAGAAATCC CTAATGGAAA TACCTTACCC
    GCCTCCTTGT TTTTCAGCTC AGAAGAAAGA AAGACTCCAG CCACATTTAA AGCTACAGTA
    CATGGGATTC CTGGGCCAGA ACCATTTACA GTGTTTGAAA TCAGTCTTGG GACAACTGCA
    AAGCAACTTC TTGATCATAT TTTGGCTACT ATACAAGGCA ATGAAGCAGA CATCACTGAC
    TATTTTTTGA TGGAGGAAAA ATGCTTTATA TCTAAAGACA AAAATGAATG CAAGAAATTG
    CCGTTCCAGA GAGTGATTAG CCCAGAAGAG GATATCCTGC AAATTTTGAA TAGCTGGTTC
    CCAGAAGAAG GCTACGTGGG GAGAATTGTT CTTAAAACAC GAGAGGAGAA CATGAATGAC
    AAAAATGTTC AAGAGGACAA AGAAGAAAAA GAGGTGACCG TGAGCTCCGA TGAGGAGACT
    TTCTTTGTCC AGGTCCATGA TGTTTCTCCT GAACAGCCAA GAACGGTTAT CAAAGCTTTA
    CGATTCAGTA CAGCGCAGGA AGTTATCCAG CAGGCTTTAT GCAAGGCTAA ATACTCATAC
    AGCATCCTGA GCAACCCTAA TCCTAGTGAT TATGTGCTTC TGGAGGAAGT CATCAAAGAA
    GCTTCAAATA AGAAGTCTTC TACCTCCAAA ACAATTCAGA GGGTTCTCTT GGATCAAGAG
    TGTGTATTCC AGGCACAGAG CAAGTGGAAG GGAGCAGGGA AATTCATTCT TAAACTGAAG
    GAACAAGTCC AGGCGGCAAG AGACGACAAG CGCAAAGGTC TGTCTATAGC CAGTGAACTG
    AAGAAGCTAG CAAAGTCCAG CCGGCAATCT CGGAACTTCA CTTTGTCACC TCAGATATTT
    TTATCAGAAG GTACCCAGAA TAGGGATGAA AAACCTGCAT GCAGTTTGTC CTTCAGTGAC
    ATCAATGAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62384
    Clone ID Related Accession (Same CDS sequence) XM_031905950.1
    Accession Version XM_031905950.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6879bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCAGTT TATTATGGGA
    GATTTATCTG ACTCTGAAAG TGATATCTTT GAACAATCAA AAGAGTGGGA CTTGCATAGG
    AGGGAAGAGC AACAGAAGGC ATTCAGCCAT GGAACAGAGC TAATTCCCTG GTATGTGTTA
    TCCATGAGAG CAGATGTCTA TCAGTTTCTT CAGCAAGGAG TGACTGTCAT CCGCTATGAC
    CAGGAGACAC ACATTTCTGT TCGTTGTTTC CTTCAACTCC AACCAGACAA CTCCACATTG
    ACTTGGACAA AACCAACCAC AACGTGTCTT GCAAACACTA AAAATAAATT AGGAACTGTA
    AGTAGCAGTG CGGAGATTAA ATTTCAGTTC CTTGCCAATG CTGGAATGAA TGGACTGGCC
    GAAGGATTCT TGGACTTATT CTCTGTAAAG GCAGTTTACA TGGGACATCC GGGAATTGAT
    ATGCACACTG TGTGTGTTCA AAACAAACTA TGTAATATGA ACCTGGAAGA AAATGGTGTC
    ACATTGCTTT ATGGGCTGCA CACTACTGAC AACAAGTTGC TGCACTTTGT AGCTCCGAAA
    TACACAGCCA GGACATTGTA TGATGGACTC CTGGAGTTAA CCAAAGCAGT AAGGAAGATA
    AAACGCTTTC CTGATCAGAG ATTACAGTGG TTAAGGAAGC AGTATGTTAG TCTCTACCAG
    GAGGAAAACA GATTTGAAGG ACCAGCTTTG GCACAAGCTA TTGAGCTGTT TGGAGGGCGA
    CGCTGGAGTA CGAGAAACAC GAGCACAGGG ACCTTAACCA AAAGCACAGA GAAACCAAAC
    GTGCAGAGGA ATAACACTCT CGGCATCAAC ACTGCCAAGA AGAAGAAGAA AGTGCTTATG
    AGGGGCGAGA GTGGGGATGC AGCCGATGAT GAGATGGCAA CCCGCAAAAC AAAAAGCTGC
    AAGGAAAGTC GCAGTAGAAG TGGCTCTGAT CCTCCAGAAA TCGATGAGCA GGAAGAGCAA
    GATCTGAATA TTATTGCTGG ATATTCACCA TCTCAAATGC TCCCATCCCG AAGAGCTCAT
    TCTATGTCGA CTTCTGGTTC TCCCAACTTG ACACCCGGGC CATCTATTCC ACTGCGACCT
    GCCTCCTCTC CTATTTTGTC CAACTCAAAC AAACCCCAGT CCAATACGTG GAGCAGTTCA
    AGTTGGCATG GAAGAGTTAA AGGTGGGATG AAAGGCTTCC AATCATTCAT GGTTTCGGAC
    AGCAACATGA ACTTTACTGA ATTTGTAGAG CTTTTCAAAT CTTTTAGCGT AAGAAGTCGG
    AAAGACCTGA AGGACATTTT TGATGTGTAT GCTGTGGCCT GCAATCGGTC AGGTGCAGAA
    TCAGTCCCTC TCTACACTAA TTTGACAATT GATGAGAATG TAGTTGGAGT CCAACCTGAT
    TTAGATTTAT TGACCAGGAA TGTGTCCGAC TTGGGTTTAT TCATTCGCAG CAGACAGCAA
    CTGTCTGAGA ACCAAAGGCA AATCTCAGAT GCCATTGCGG CTGCCAGCAT TGTAACTAAT
    GGAACTGGAG TAGAAAGCAC ATCCCTGGGC ATATTTGGAA TGGGAATACT GCAGTTTAAT
    GATTTCCTGG TGAATTGCCA GGGTGAACAT TACACCTATG ATGAAGTTCT TAGCATTATT
    CAGAAATTTG AACCAAGTAT CAGTATGCGC CAGCAGGGGC TGATGTCATT TGAAGGATTT
    GCAAGGTTCC TGATGGATAA AGACAATTTT GCATCAAGAA ATGATGAGTC TCAGGTAAAC
    ACAGAAGAAC TTCAGCTGCC CCTTTCCTAC TATTTCATTG AGTCTTCACA CAACACCTAC
    CTTACAGGGC ACCAGCTAAA AGGAGAGTCT TCAGTTGAGC TGTACAGCCA GGTCCTTTTG
    CAGGGGTGCC GAAGTGTTGA ATTAGACTGC TGGGATGGAG ATGACGGCAT GCCTATCATT
    TATCATGGAC ATACACTAAC TACTAAAATT CCCTTTAAGG ATGTCATTGA AGCTATTGGC
    CGAAGTGCAT TTATTACCTC TGAAATGCCA ATCGTACTGT CCATAGAAAA CCATTGCTCC
    CTACCACAGC AGCGTAAAAT GGCAGATATA TTTAAGAATG TCTTTGGTGA AAAACTGGTG
    ACAAAGTTCC TGTTTGAAAG TGACTTTTCA GATGATCCGA TGTTGCCTTC TCCATGGCAA
    TTGAGAAATA AAGTGCTTCT GAAAAATAAA AAGCTGAAGG CACATCAAAC CCCGGTGGAT
    ATACTGAAGC AGAAGGCACA TCAGCTGGCT AGCATGCAAG CACAAGCAAG CAATGGTAGC
    CAAATGGTAA GCCCTAGCAC AAACAATGAA GAAGAAGAAG ATGAAGAAGA TGAATATGAT
    TATGACTATG AGTCTCTGTC AGATGATAAT ATTCTTGATG ACCGATCTGA AACTAAAACC
    AACAGCGACA AACTGCAATT TGAATATAAT GAGGAAGCTT CAAAGCGAAT CAAAAAGACA
    GATGGCAGCA GTATTAACAC TAAAGGAAAG GTCTATGACA TGGAGTTGGG TGAGGAATTC
    TATCTTCCAC AAAACAAAAA AGAGAGCAGG CAAATTGCAC CTGAATTGTC AGACCTTATT
    ATCTATTGCC AAGCTGTCAA ATTCCCAGGC CTTACAACAT TAAACCCATG TGGATCTGGC
    AGAGGGAAGG AAAGAAAAAG CAGGAAGTCC ATTTTTGGCA ACAACCCTGG CAGAACAAGT
    CCTGGGGAAC CAACAGCACT TGCCAAAACA TCTGGAAAAG GTACAAATGA AACACGGCAG
    AGCTGGGAAG AGCCATGTTC TCCTCCCTTT AACCCCAGCA CTTCCCTCAG TGCAATAATC
    AGGACACCCA GGTGCTACCA TATCTCTTCC CTAAATGAGA ACGCAGCCAA GCGCTTGTGC
    CGAAGGTATT CTCAGAAACT CATTCAACAT ACCGCATATC AACTGCTCCG AACATATCCC
    GCCGCCACTC GGATAGACTC TTCCAACCCA CATCCTCTTA TTTTCTGGCT TCATGGTGTA
    CAGTTGGTTG CTCTAAACTA CCAAACTGAT GATCTCCCCT TACATCTAAA CGCCGCCATG
    TTTGAAGCAA ATGGTGGTTG CGGATATGTG TTAAAGCCCC CCGTGCAGTG GGATAGGACC
    TGCCCAATGT ACCAATTATT CTCACCATTA GAACGAGATT TAGAAAATAT GGAACCAGCA
    ATATATTCTT TGACGATAGT CTCTGGGCAA AATGTGTGTC CAGGTAACAG CTCAGGCAGC
    CCCTGCATTG AAATTGATGT CTTGGGTATG CCAGTAGACA GCTGCCATTT TCGGACCAAA
    CCAATCCATC GCAATACCTT AAATCCCATG TGGAATGAAC AATTCTTATT CCGAATATAC
    TTTGAGGATC TTGTGTTTCT TCGGTTTGCT GTGGTGGAAA ACAACAGCTC TGCTGTAACT
    GCCCAAAGAA TAATTAATCT TAAAGCTTTA AAGAGAGGTA ACAGACACCT TCAGCTAAGA
    AATTTACATA ATGAGCCCTT AGAGGTTTCT ACCTTATTTA TAAATAGCAG GAGAATGGAA
    GAAATCCCTA ATGGAAATAC CTTACCCGCC TCCTTGTTTT TCAGCTCAGA AGAAAGAAAG
    ACTCCAGCCA CATTTAAAGC TACAGTACAT GGGATTCCTG GGCCAGAACC ATTTACAGTG
    TTTGAAATCA GTCTTGGGAC AACTGCAAAG CAACTTCTTG ATCATATTTT GGCTACTATA
    CAAGGCAATG AAGCAGACAT CACTGACTAT TTTTTGATGG AGGAAAAATG CTTTATATCT
    AAAGACAAAA ATGAATGCAA GAAATTGCCG TTCCAGAGAG TGATTAGCCC AGAAGAGGAT
    ATCCTGCAAA TTTTGAATAG CTGGTTCCCA GAAGAAGGCT ACGTGGGGAG AATTGTTCTT
    AAAACACGAG AGGAGAACAT GAATGACAAA AATGTTCAAG AGGACAAAGA AGAAAAAGAG
    GTGACCGTGA GCTCCGATGA GGAGACTTTC TTTGTCCAGG TCCATGATGT TTCTCCTGAA
    CAGCCAAGAA CGGTTATCAA AGCTTTACGA TTCAGTACAG CGCAGGAAGT TATCCAGCAG
    GCTTTATGCA AGGCTAAATA CTCATACAGC ATCCTGAGCA ACCCTAATCC TAGTGATTAT
    GTGCTTCTGG AGGAAGTCAT CAAAGAAGCT TCAAATAAGA AGTCTTCTAC CTCCAAAACA
    ATTCAGAGGG TTCTCTTGGA TCAAGAGTGT GTATTCCAGG CACAGAGCAA GTGGAAGGGA
    GCAGGGAAAT TCATTCTTAA ACTGAAGGAA CAAGTCCAGG CGGCAAGAGA CGACAAGCGC
    AAAGGTCTGT CTATAGCCAG TGAACTGAAG AAGCTAGCAA AGTCCAGCCG GCAATCTCGG
    AACTTCACTT TGTCACCTCA GATATTTTTA TCAGAAGGTA CCCAGAATAG GGATGAAAAA
    CCTGCATGCA GTTTGTCCTT CAGTGACATC AATGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761810.1
    CDS566..7444
    Translation

    Target ORF information:

    RefSeq Version XM_031905950.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905950.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCAGTT TATTATGGGA
    GATTTATCTG ACTCTGAAAG TGATATCTTT GAACAATCAA AAGAGTGGGA CTTGCATAGG
    AGGGAAGAGC AACAGAAGGC ATTCAGCCAT GGAACAGAGC TAATTCCCTG GTATGTGTTA
    TCCATGAGAG CAGATGTCTA TCAGTTTCTT CAGCAAGGAG TGACTGTCAT CCGCTATGAC
    CAGGAGACAC ACATTTCTGT TCGTTGTTTC CTTCAACTCC AACCAGACAA CTCCACATTG
    ACTTGGACAA AACCAACCAC AACGTGTCTT GCAAACACTA AAAATAAATT AGGAACTGTA
    AGTAGCAGTG CGGAGATTAA ATTTCAGTTC CTTGCCAATG CTGGAATGAA TGGACTGGCC
    GAAGGATTCT TGGACTTATT CTCTGTAAAG GCAGTTTACA TGGGACATCC GGGAATTGAT
    ATGCACACTG TGTGTGTTCA AAACAAACTA TGTAATATGA ACCTGGAAGA AAATGGTGTC
    ACATTGCTTT ATGGGCTGCA CACTACTGAC AACAAGTTGC TGCACTTTGT AGCTCCGAAA
    TACACAGCCA GGACATTGTA TGATGGACTC CTGGAGTTAA CCAAAGCAGT AAGGAAGATA
    AAACGCTTTC CTGATCAGAG ATTACAGTGG TTAAGGAAGC AGTATGTTAG TCTCTACCAG
    GAGGAAAACA GATTTGAAGG ACCAGCTTTG GCACAAGCTA TTGAGCTGTT TGGAGGGCGA
    CGCTGGAGTA CGAGAAACAC GAGCACAGGG ACCTTAACCA AAAGCACAGA GAAACCAAAC
    GTGCAGAGGA ATAACACTCT CGGCATCAAC ACTGCCAAGA AGAAGAAGAA AGTGCTTATG
    AGGGGCGAGA GTGGGGATGC AGCCGATGAT GAGATGGCAA CCCGCAAAAC AAAAAGCTGC
    AAGGAAAGTC GCAGTAGAAG TGGCTCTGAT CCTCCAGAAA TCGATGAGCA GGAAGAGCAA
    GATCTGAATA TTATTGCTGG ATATTCACCA TCTCAAATGC TCCCATCCCG AAGAGCTCAT
    TCTATGTCGA CTTCTGGTTC TCCCAACTTG ACACCCGGGC CATCTATTCC ACTGCGACCT
    GCCTCCTCTC CTATTTTGTC CAACTCAAAC AAACCCCAGT CCAATACGTG GAGCAGTTCA
    AGTTGGCATG GAAGAGTTAA AGGTGGGATG AAAGGCTTCC AATCATTCAT GGTTTCGGAC
    AGCAACATGA ACTTTACTGA ATTTGTAGAG CTTTTCAAAT CTTTTAGCGT AAGAAGTCGG
    AAAGACCTGA AGGACATTTT TGATGTGTAT GCTGTGGCCT GCAATCGGTC AGGTGCAGAA
    TCAGTCCCTC TCTACACTAA TTTGACAATT GATGAGAATG TAGTTGGAGT CCAACCTGAT
    TTAGATTTAT TGACCAGGAA TGTGTCCGAC TTGGGTTTAT TCATTCGCAG CAGACAGCAA
    CTGTCTGAGA ACCAAAGGCA AATCTCAGAT GCCATTGCGG CTGCCAGCAT TGTAACTAAT
    GGAACTGGAG TAGAAAGCAC ATCCCTGGGC ATATTTGGAA TGGGAATACT GCAGTTTAAT
    GATTTCCTGG TGAATTGCCA GGGTGAACAT TACACCTATG ATGAAGTTCT TAGCATTATT
    CAGAAATTTG AACCAAGTAT CAGTATGCGC CAGCAGGGGC TGATGTCATT TGAAGGATTT
    GCAAGGTTCC TGATGGATAA AGACAATTTT GCATCAAGAA ATGATGAGTC TCAGGTAAAC
    ACAGAAGAAC TTCAGCTGCC CCTTTCCTAC TATTTCATTG AGTCTTCACA CAACACCTAC
    CTTACAGGGC ACCAGCTAAA AGGAGAGTCT TCAGTTGAGC TGTACAGCCA GGTCCTTTTG
    CAGGGGTGCC GAAGTGTTGA ATTAGACTGC TGGGATGGAG ATGACGGCAT GCCTATCATT
    TATCATGGAC ATACACTAAC TACTAAAATT CCCTTTAAGG ATGTCATTGA AGCTATTGGC
    CGAAGTGCAT TTATTACCTC TGAAATGCCA ATCGTACTGT CCATAGAAAA CCATTGCTCC
    CTACCACAGC AGCGTAAAAT GGCAGATATA TTTAAGAATG TCTTTGGTGA AAAACTGGTG
    ACAAAGTTCC TGTTTGAAAG TGACTTTTCA GATGATCCGA TGTTGCCTTC TCCATGGCAA
    TTGAGAAATA AAGTGCTTCT GAAAAATAAA AAGCTGAAGG CACATCAAAC CCCGGTGGAT
    ATACTGAAGC AGAAGGCACA TCAGCTGGCT AGCATGCAAG CACAAGCAAG CAATGGTAGC
    CAAATGGTAA GCCCTAGCAC AAACAATGAA GAAGAAGAAG ATGAAGAAGA TGAATATGAT
    TATGACTATG AGTCTCTGTC AGATGATAAT ATTCTTGATG ACCGATCTGA AACTAAAACC
    AACAGCGACA AACTGCAATT TGAATATAAT GAGGAAGCTT CAAAGCGAAT CAAAAAGACA
    GATGGCAGCA GTATTAACAC TAAAGGAAAG GTCTATGACA TGGAGTTGGG TGAGGAATTC
    TATCTTCCAC AAAACAAAAA AGAGAGCAGG CAAATTGCAC CTGAATTGTC AGACCTTATT
    ATCTATTGCC AAGCTGTCAA ATTCCCAGGC CTTACAACAT TAAACCCATG TGGATCTGGC
    AGAGGGAAGG AAAGAAAAAG CAGGAAGTCC ATTTTTGGCA ACAACCCTGG CAGAACAAGT
    CCTGGGGAAC CAACAGCACT TGCCAAAACA TCTGGAAAAG GTACAAATGA AACACGGCAG
    AGCTGGGAAG AGCCATGTTC TCCTCCCTTT AACCCCAGCA CTTCCCTCAG TGCAATAATC
    AGGACACCCA GGTGCTACCA TATCTCTTCC CTAAATGAGA ACGCAGCCAA GCGCTTGTGC
    CGAAGGTATT CTCAGAAACT CATTCAACAT ACCGCATATC AACTGCTCCG AACATATCCC
    GCCGCCACTC GGATAGACTC TTCCAACCCA CATCCTCTTA TTTTCTGGCT TCATGGTGTA
    CAGTTGGTTG CTCTAAACTA CCAAACTGAT GATCTCCCCT TACATCTAAA CGCCGCCATG
    TTTGAAGCAA ATGGTGGTTG CGGATATGTG TTAAAGCCCC CCGTGCAGTG GGATAGGACC
    TGCCCAATGT ACCAATTATT CTCACCATTA GAACGAGATT TAGAAAATAT GGAACCAGCA
    ATATATTCTT TGACGATAGT CTCTGGGCAA AATGTGTGTC CAGGTAACAG CTCAGGCAGC
    CCCTGCATTG AAATTGATGT CTTGGGTATG CCAGTAGACA GCTGCCATTT TCGGACCAAA
    CCAATCCATC GCAATACCTT AAATCCCATG TGGAATGAAC AATTCTTATT CCGAATATAC
    TTTGAGGATC TTGTGTTTCT TCGGTTTGCT GTGGTGGAAA ACAACAGCTC TGCTGTAACT
    GCCCAAAGAA TAATTAATCT TAAAGCTTTA AAGAGAGGTA ACAGACACCT TCAGCTAAGA
    AATTTACATA ATGAGCCCTT AGAGGTTTCT ACCTTATTTA TAAATAGCAG GAGAATGGAA
    GAAATCCCTA ATGGAAATAC CTTACCCGCC TCCTTGTTTT TCAGCTCAGA AGAAAGAAAG
    ACTCCAGCCA CATTTAAAGC TACAGTACAT GGGATTCCTG GGCCAGAACC ATTTACAGTG
    TTTGAAATCA GTCTTGGGAC AACTGCAAAG CAACTTCTTG ATCATATTTT GGCTACTATA
    CAAGGCAATG AAGCAGACAT CACTGACTAT TTTTTGATGG AGGAAAAATG CTTTATATCT
    AAAGACAAAA ATGAATGCAA GAAATTGCCG TTCCAGAGAG TGATTAGCCC AGAAGAGGAT
    ATCCTGCAAA TTTTGAATAG CTGGTTCCCA GAAGAAGGCT ACGTGGGGAG AATTGTTCTT
    AAAACACGAG AGGAGAACAT GAATGACAAA AATGTTCAAG AGGACAAAGA AGAAAAAGAG
    GTGACCGTGA GCTCCGATGA GGAGACTTTC TTTGTCCAGG TCCATGATGT TTCTCCTGAA
    CAGCCAAGAA CGGTTATCAA AGCTTTACGA TTCAGTACAG CGCAGGAAGT TATCCAGCAG
    GCTTTATGCA AGGCTAAATA CTCATACAGC ATCCTGAGCA ACCCTAATCC TAGTGATTAT
    GTGCTTCTGG AGGAAGTCAT CAAAGAAGCT TCAAATAAGA AGTCTTCTAC CTCCAAAACA
    ATTCAGAGGG TTCTCTTGGA TCAAGAGTGT GTATTCCAGG CACAGAGCAA GTGGAAGGGA
    GCAGGGAAAT TCATTCTTAA ACTGAAGGAA CAAGTCCAGG CGGCAAGAGA CGACAAGCGC
    AAAGGTCTGT CTATAGCCAG TGAACTGAAG AAGCTAGCAA AGTCCAGCCG GCAATCTCGG
    AACTTCACTT TGTCACCTCA GATATTTTTA TCAGAAGGTA CCCAGAATAG GGATGAAAAA
    CCTGCATGCA GTTTGTCCTT CAGTGACATC AATGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62385
    Clone ID Related Accession (Same CDS sequence) XM_031905951.1
    Accession Version XM_031905951.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCAGTT TATTATGGGA
    GATTTATCTG ACTCTGAAAG TGATATCTTT GAACAATCAA AAGAGTGGGA CTTGCATAGG
    AGGGAAGAGC AACAGAAGGC ATTCAGCCAT GGAACAGAGC TAATTCCCTG GTATGTGTTA
    TCCATGAGAG CAGATGTCTA TCAGTTTCTT CAGCAAGGAG TGACTGTCAT CCGCTATGAC
    CAGGAGACAC ACATTTCTGT TCGTTGTTTC CTTCAACTCC AACCAGACAA CTCCACATTG
    ACTTGGACAA AACCAACCAC AACGTGTCTT GCAAACACTA AAAATAAATT AGGAACTGTA
    AGTAGCAGTG CGGAGATTAA ATTTCAGTTC CTTGCCAATG CTGGAATGAA TGGACTGGCC
    GAAGGATTCT TGGACTTATT CTCTGTAAAG GCAGTTTACA TGGGACATCC GGGAATTGAT
    ATGCACACTG TGTGTGTTCA AAACAAACTA TGTAATATGA ACCTGGAAGA AAATGGTGTC
    ACATTGCTTT ATGGGCTGCA CACTACTGAC AACAAGTTGC TGCACTTTGT AGCTCCGAAA
    TACACAGCCA GGACATTGTA TGATGGACTC CTGGAGTTAA CCAAAGCAGT AAGGAAGATA
    AAACGCTTTC CTGATCAGAG ATTACAGTGG TTAAGGAAGC AGTATGTTAG TCTCTACCAG
    GAGGAAAACA GATTTGAAGG ACCAGCTTTG GCACAAGCTA TTGAGCTGTT TGGAGGGCGA
    CGCTGGAGTA CGAGAAACAC GAGCACAGGG ACCTTAACCA AAAGCACAGA GAAACCAAAC
    GTGCAGAGGA ATAACACTCT CGGCATCAAC ACTGCCAAGA AGAAGAAGAA AGTGCTTATG
    AGGGGCGAGA GTGGGGATGC AGCCGATGAT GAGATGGCAA CCCGCAAAAC AAAAAGCTGC
    AAGGAAAGTC GCAGTAGAAG TGGCTCTGAT CCTCCAGAAA TCGATGAGCA GGAAGAGCAA
    GATCTGAATA TTATTGCTGG ATATTCACCA TCTCAAATGC TCCCATCCCG AAGAGCTCAT
    TCTATGTCGA CTTCTGGTTC TCCCAACTTG ACACCCGGGC CATCTATTCC ACTGCGACCT
    GCCTCCTCTC CTATTTTGTC CAACTCAAAC AAACCCCAGT CCAATACGTG GAGCAGTTCA
    AGTTGGCATG GAAGAGTTAA AGGTGGGATG AAAGGCTTCC AATCATTCAT GGTTTCGGAC
    AGCAACATGA ACTTTACTGA ATTTGTAGAG CTTTTCAAAT CTTTTAGCGT AAGAAGTCGG
    AAAGACCTGA AGGACATTTT TGATGTGTAT GCTGTGGCCT GCAATCGGTC AGGTGCAGAA
    TCAGTCCCTC TCTACACTAA TTTGACAATT GATGAGAATG TAGTTGGAGT CCAACCTGAT
    TTAGATTTAT TGACCAGGAA TGTGTCCGAC TTGGGTTTAT TCATTCGCAG CAGACAGCAA
    CTGTCTGAGA ACCAAAGGCA AATCTCAGAT GCCATTGCGG CTGCCAGCAT TGTAACTAAT
    GGAACTGGAG TAGAAAGCAC ATCCCTGGGC ATATTTGGAA TGGGAATACT GCAGTTTAAT
    GATTTCCTGG TGAATTGCCA GGGTGAACAT TACACCTATG ATGAAGTTCT TAGCATTATT
    CAGAAATTTG AACCAAGTAT CAGTATGCGC CAGCAGGGGC TGATGTCATT TGAAGGATTT
    GCAAGGTTCC TGATGGATAA AGACAATTTT GCATCAAGAA ATGATGAGTC TCAGGTAAAC
    ACAGAAGAAC TTCAGCTGCC CCTTTCCTAC TATTTCATTG AGTCTTCACA CAACACCTAC
    CTTACAGGGC ACCAGCTAAA AGGAGAGTCT TCAGTTGAGC TGTACAGCCA GGTCCTTTTG
    CAGGGGTGCC GAAGTGTTGA ATTAGACTGC TGGGATGGAG ATGACGGCAT GCCTATCATT
    TATCATGGAC ATACACTAAC TACTAAAATT CCCTTTAAGG ATGTCATTGA AGCTATTGGC
    CGAAGTGCAT TTATTACCTC TGAAATGCCA ATCGTACTGT CCATAGAAAA CCATTGCTCC
    CTACCACAGC AGCGTAAAAT GGCAGATATA TTTAAGAATG TCTTTGGTGA AAAACTGGTG
    ACAAAGTTCC TGTTTGAAAG TGACTTTTCA GATGATCCGA TGTTGCCTTC TCCATGGCAA
    TTGAGAAATA AAGTGCTTCT GAAAAATAAA AAGCTGAAGG CACATCAAAC CCCGGTGGAT
    ATACTGAAGC AGAAGGCACA TCAGCTGGCT AGCATGCAAG CACAAGCAAG CAATGGTAGC
    CAAATGGTAA GCCCTAGCAC AAACAATGAA GAAGAAGAAG ATGAAGAAGA TGAATATGAT
    TATGACTATG AGTCTCTGTC AGATGCCGAT GTCCTTAATG CTTCCCCTGC TCCTAACAGC
    CAGGAAGATA ATATTCTTGA TGACCGATCT GAAACTAAAA CCAACAGCGA CAAACTGCAA
    TTTGAATATA ATGAGGAAGC TTCAAAGCGA ATCAAAAAGA CAGATGGCAG CAGTATTAAC
    ACTAAAGGAA AGGTCTATGA CATGGAGTTG GGTGAGGAAT TCTATCTTCC ACAAAACAAA
    AAAGAGAGCA GGCAAATTGC ACCTGAATTG TCAGACCTTA TTATCTATTG CCAAGCTGTC
    AAATTCCCAG GCCTTACAAC ATTAAACCCA TGTGGATCTG GCAGAGGGAA GGAAAGAAAA
    AGCAGGAAGT CCATTTTTGG CAACAACCCT GGCAGAACAA GTCCTGGGGA ACCAACAGCA
    CTTGCCAAAA CATCTGGAAA AGGTACAAAT GAAACACGGC AGAGCTGGGA AGAGCCATGT
    TCTCCTCCCT TTAACCCCAG CACTTCCCTC AGTGCAATAA TCAGGACACC CAGGTGCTAC
    CATATCTCTT CCCTAAATGA GAACGCAGCC AAGCGCTTGT GCCGAAGGTA TTCTCAGAAA
    CTCATTCAAC ATACCGCATA TCAACTGCTC CGAACATATC CCGCCGCCAC TCGGATAGAC
    TCTTCCAACC CACATCCTCT TATTTTCTGG CTTCATGGTG TACAGTTGGT TGCTCTAAAC
    TACCAAACTG ATGATCTCCC CTTACATCTA AACGCCGCCA TGTTTGAAGC AAATGGTGGT
    TGCGGATATG TGTTAAAGCC CCCCGTGCAG TGGGATAGGA CCTGCCCAAT GTACCAATTA
    TTCTCACCAT TAGAACGAGA TTTAGAAAAT ATGGAACCAG CAATATATTC TTTGACGATA
    GTCTCTGGGC AAAATGTGTG TCCAGGTAAC AGCTCAGGCA GCCCCTGCAT TGAAATTGAT
    GTCTTGGGTA TGCCAGTAGA CAGCTGCCAT TTTCGGACCA AACCAATCCA TCGCAATACC
    TTAAATCCCA TGTGGAATGA ACAATTCTTA TTCCGAATAT ACTTTGAGGA TCTTGTGTTT
    CTTCGGTTTG CTGTGGTGGA AAACAACAGC TCTGCTGTAA CTGCCCAAAG AATAATTAAT
    CTTAAAGCTT TAAAGAGAGG TAACAGACAC CTTCAGCTAA GAAATTTACA TAATGAGCCC
    TTAGAGGTTT CTACCTTATT TATAAATAGC AGGAGAATGG AAGAAATCCC TAATGGAAAT
    ACCTTACCCG CCTCCTTGTT TTTCAGCTCA GAAGAAAGAA AGACTCCAGC CACATTTAAA
    GCTACAGTAC ATGGGATTCC TGGGCCAGAA CCATTTACAG TGTTTGAAAT CAGTCTTGGG
    ACAACTGCAA AGCAACTTCT TGATCATATT TTGGCTACTA TACAAGGCAA TGAAGCAGAC
    ATCACTGACT ATTTTTTGAT GGAGGAAAAA TGCTTTATAT CTAAAGACAA AAATGAATGC
    AAGAAATTGC CGTTCCAGAG AGTGATTAGC CCAGAAGAGG ATATCCTGCA AATTTTGAAT
    AGCTGGTTCC CAGAAGAAGG CTACGTGGGG AGAATTGTTC TTAAAACACG AGAGGAGAAC
    ATGAATGACA AAAATGTTCA AGAGGACAAA GAAGAAAAAG AGGTGACCGT GAGCTCCGAT
    GAGGAGACTT TCTTTGTCCA GGTCCATGAT GTTTCTCCTG AACAGCCAAG AACGGTTATC
    AAAGCTTTAC GATTCAGTAC AGCGCAGGAA GTTATCCAGC AGGCTTTATG CAAGGCTAAA
    TACTCATACA GCATCCTGAG CAACCCTAAT CCTAGTGATT ATGTGCTTCT GGAGGAAGTC
    ATCAAAGAAG CTTCAAATAA GAAGTCTTCT ACCTCCAAAA CAATTCAGAG GGTTCTCTTG
    GATCAAGAGT GTGTATTCCA GGCACAGAGC AAGTGGAAGG GAGCAGGGAA ATTCATTCTT
    AAACTGAAGG AACAAGTCCA GGCGGCAAGA GACGACAAGC GCAAAGGTCT GTCTATAGCC
    AGTGAACTGA AGAAGCTAGC AAAGTCCAGC CGGCAATCTC GGAACTTCAC TTTGTCACCT
    CAGATATTTT TATCAGAAGG TACCCAGAAT AGGGATGAAA AACCTGCATG CAGTTTGTCC
    TTCAGTGACA TCAATGAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761811.1
    CDS566..7486
    Translation

    Target ORF information:

    RefSeq Version XM_031905951.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C epsilon 1 (plce1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905951.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGACCTCGA AAGATCTAGC AGCTTCTGTT GTTCTACCAG CCGCCAGGAG GAAAGTGATA 
    TGCACTCCGC CAGCTGGAGA TGAAAATTCT AGGAATACTT TGGATACTAT GGATATAAAA
    AGACAGGCAA CAGAACAAGA ACAAGAGAGA AATGAGAGCA ACCCCCATAC AAATCAGTTA
    AAGGAGCACT ACCTTCCAAA CTCCTTCATT AGGACTTTTT CACCAGGAAA TAAAGATGTA
    GGCAGAGAAA ACCTTCCTGC AGAAAAAGAC AAGTGTTTAC CCAATTCAGT GGAATATCCC
    AACATCAACT GCAAGATTTT GGAGAAAGCC TGCAAAGCAA ACTTGCATTT TAATCAGTGT
    TACAAGTCCT ACAATCCTCT AAAAGGGAAG GGCACGTGTG CAGAACAGAG GATTCCTGCA
    GTGCTGACAG AAATTAATTT TAAAGTAGAC AGAACTTCAG TGGGCGATGG GGCTTTAAAG
    GGTCGGCTGG AAGGGAATGA GACTTGCACA GATGCTGTCA GTCACATTCC TGCATTACAC
    ATCAGTTATC CCCCTGGAAA GAGACTATCA AATGTTTCAT GTACCACAAA TAACAGCTAT
    ATTTTGGGTA ACCGCTCTGA AACAGTACAA AGACCTTGCA GCAGTCGACA TGCAGTTTGT
    TCTTACAAGA TGGCTCAGCC CTCCCAGAGT TGTGCCGTTT CCAACAGACC CCCAGTTATC
    ACTTGTAAAG CAGCCCACCA TTCAGATAAT GGTTCAGAGA TCAAATCGGA CCCATGTTTG
    ATACTTCAAA GGGACACTTT CTGCGGACAC ACCTTTCCCT TTTCCATGTC CTCTAACCCA
    TCTCAGAAGG CTTCAGAAGA ACCGGGCAAA TTATTGGCAG AAGAAGGACA ATACGATTTG
    AATGACTTTT CCAGGAAGAG GAAAGATCGC TCAACGTTAC TTTTTAGAAG GTTTTGTAAA
    AATGACAAGG AAGTAAAAAA GTCTATTTAT ACAGGAACAA ATGCAATTAT TAAAATGCTA
    CCCTCAGGTT ACATGGGAAC AAAGGTTTGC TCTCTGGAGA GGAGCCATGA AACCACAGGG
    GATGAGAAGG ATTATTGGTT TGTCTCTGGG GATTTAACAT CTCTTCATGT AACGCTCAGA
    AGAAAGGATT TACTTGAGTA TTTGGTGAAA ACCACTATAT TACAGTTTTC CAATGCAGGC
    AAATCGGTAG ACGATACAGA TGAACTTGCT CTGTGTCCGC TTGAAAAACG CCTAATTGCC
    GTCTCACTTC ATGGCCAGAA GGTTTGTTCC ACATGCTCAA GGCAAGATGT GCACTCGGCT
    TTCTTTGCAA CTCTGCACAG GATCTCAGTT TCAGACTTTA TAACCGGAGC TTTGTTGGAA
    GCAACTACAT CCTTGGGTGC ACGTAGTGGG GGTTTGAACA TATTTCTTGG CTCAACTGGA
    AGGACAATGT TGAAAGAACG CCATCCGTGT TCAACTATGG CAACATCAAG TTCATCACCG
    GCCAGTGCAG CTGGACTAAG CAAAGAACTG GTGGATCTTC AACACCTCAT TCAGTTTCCA
    GAGGAGATTG CAAGCATCCT CACAGAGCAA GAACAGGAGA TTTACCGGAA AGTGTTGCCT
    GTTGACTACC TCTATTTCTT GACCAAAGAT CTTAGCAACG GAGAATGTGA CACTAACCTG
    TCAGATATAA AGACATCACT GTCCGCTTCA CTAAGAAAAC TAAAGAATGG AGAGCACGAT
    GCAGTGGAAG GACTTGTGGC AAGATTTAAT GAGGTTAGCT CCTGGGTCAC ATGGCTGATT
    CTAACGGCAG GATCTATGGA GGAGAAACGA GAAGTTTTCT CACACGTCGT TCACATTGCA
    AAGTGTTGCT GGAACATGGG CAATTACAAC GCTGTCATGG AATTCCTGGC AGGCCTCAGG
    ACAAGAAAAG TTCTAAAAAT GTGGCAATTT ATGGATCAGT CCGACATTGA AACGATGCGC
    AGCTTGAAAG ATGCAATGGC ACAGCATGAA TCCTCATCCG AGTACAGGAA AGTGGTGAAC
    AGGGCCCTAA ATATACCTGG CTGTAAGGTG GTTCCTTTTT GTGGGGTCTT TCTAAAGGAA
    CTCTGTGAAG TCCTTGATGG AGCAGCGAGT ATCATCAGTC TGTGTCCTCA GTACGATGCT
    CAGAGTGAAA CACTGGAGTT TGTTTCAGAT TACAATGGGC AAGACAACTT TCTCCAGCGA
    ATAGGGAAAG ATGGATTAAA GAACACAGAG AAAGAGTCCA CTGTGAATAG TATCATGCAG
    ACAATTCGTA GCTGTAACCG TAGCTTGGAA TCTGAAGAAG GAGAAGACAA TCTCAGCGAT
    GCCGGGGGTA TCAGGAAAAG CACAGTGATG GACAGAACTA GATTTCAGTT TATTATGGGA
    GATTTATCTG ACTCTGAAAG TGATATCTTT GAACAATCAA AAGAGTGGGA CTTGCATAGG
    AGGGAAGAGC AACAGAAGGC ATTCAGCCAT GGAACAGAGC TAATTCCCTG GTATGTGTTA
    TCCATGAGAG CAGATGTCTA TCAGTTTCTT CAGCAAGGAG TGACTGTCAT CCGCTATGAC
    CAGGAGACAC ACATTTCTGT TCGTTGTTTC CTTCAACTCC AACCAGACAA CTCCACATTG
    ACTTGGACAA AACCAACCAC AACGTGTCTT GCAAACACTA AAAATAAATT AGGAACTGTA
    AGTAGCAGTG CGGAGATTAA ATTTCAGTTC CTTGCCAATG CTGGAATGAA TGGACTGGCC
    GAAGGATTCT TGGACTTATT CTCTGTAAAG GCAGTTTACA TGGGACATCC GGGAATTGAT
    ATGCACACTG TGTGTGTTCA AAACAAACTA TGTAATATGA ACCTGGAAGA AAATGGTGTC
    ACATTGCTTT ATGGGCTGCA CACTACTGAC AACAAGTTGC TGCACTTTGT AGCTCCGAAA
    TACACAGCCA GGACATTGTA TGATGGACTC CTGGAGTTAA CCAAAGCAGT AAGGAAGATA
    AAACGCTTTC CTGATCAGAG ATTACAGTGG TTAAGGAAGC AGTATGTTAG TCTCTACCAG
    GAGGAAAACA GATTTGAAGG ACCAGCTTTG GCACAAGCTA TTGAGCTGTT TGGAGGGCGA
    CGCTGGAGTA CGAGAAACAC GAGCACAGGG ACCTTAACCA AAAGCACAGA GAAACCAAAC
    GTGCAGAGGA ATAACACTCT CGGCATCAAC ACTGCCAAGA AGAAGAAGAA AGTGCTTATG
    AGGGGCGAGA GTGGGGATGC AGCCGATGAT GAGATGGCAA CCCGCAAAAC AAAAAGCTGC
    AAGGAAAGTC GCAGTAGAAG TGGCTCTGAT CCTCCAGAAA TCGATGAGCA GGAAGAGCAA
    GATCTGAATA TTATTGCTGG ATATTCACCA TCTCAAATGC TCCCATCCCG AAGAGCTCAT
    TCTATGTCGA CTTCTGGTTC TCCCAACTTG ACACCCGGGC CATCTATTCC ACTGCGACCT
    GCCTCCTCTC CTATTTTGTC CAACTCAAAC AAACCCCAGT CCAATACGTG GAGCAGTTCA
    AGTTGGCATG GAAGAGTTAA AGGTGGGATG AAAGGCTTCC AATCATTCAT GGTTTCGGAC
    AGCAACATGA ACTTTACTGA ATTTGTAGAG CTTTTCAAAT CTTTTAGCGT AAGAAGTCGG
    AAAGACCTGA AGGACATTTT TGATGTGTAT GCTGTGGCCT GCAATCGGTC AGGTGCAGAA
    TCAGTCCCTC TCTACACTAA TTTGACAATT GATGAGAATG TAGTTGGAGT CCAACCTGAT
    TTAGATTTAT TGACCAGGAA TGTGTCCGAC TTGGGTTTAT TCATTCGCAG CAGACAGCAA
    CTGTCTGAGA ACCAAAGGCA AATCTCAGAT GCCATTGCGG CTGCCAGCAT TGTAACTAAT
    GGAACTGGAG TAGAAAGCAC ATCCCTGGGC ATATTTGGAA TGGGAATACT GCAGTTTAAT
    GATTTCCTGG TGAATTGCCA GGGTGAACAT TACACCTATG ATGAAGTTCT TAGCATTATT
    CAGAAATTTG AACCAAGTAT CAGTATGCGC CAGCAGGGGC TGATGTCATT TGAAGGATTT
    GCAAGGTTCC TGATGGATAA AGACAATTTT GCATCAAGAA ATGATGAGTC TCAGGTAAAC
    ACAGAAGAAC TTCAGCTGCC CCTTTCCTAC TATTTCATTG AGTCTTCACA CAACACCTAC
    CTTACAGGGC ACCAGCTAAA AGGAGAGTCT TCAGTTGAGC TGTACAGCCA GGTCCTTTTG
    CAGGGGTGCC GAAGTGTTGA ATTAGACTGC TGGGATGGAG ATGACGGCAT GCCTATCATT
    TATCATGGAC ATACACTAAC TACTAAAATT CCCTTTAAGG ATGTCATTGA AGCTATTGGC
    CGAAGTGCAT TTATTACCTC TGAAATGCCA ATCGTACTGT CCATAGAAAA CCATTGCTCC
    CTACCACAGC AGCGTAAAAT GGCAGATATA TTTAAGAATG TCTTTGGTGA AAAACTGGTG
    ACAAAGTTCC TGTTTGAAAG TGACTTTTCA GATGATCCGA TGTTGCCTTC TCCATGGCAA
    TTGAGAAATA AAGTGCTTCT GAAAAATAAA AAGCTGAAGG CACATCAAAC CCCGGTGGAT
    ATACTGAAGC AGAAGGCACA TCAGCTGGCT AGCATGCAAG CACAAGCAAG CAATGGTAGC
    CAAATGGTAA GCCCTAGCAC AAACAATGAA GAAGAAGAAG ATGAAGAAGA TGAATATGAT
    TATGACTATG AGTCTCTGTC AGATGCCGAT GTCCTTAATG CTTCCCCTGC TCCTAACAGC
    CAGGAAGATA ATATTCTTGA TGACCGATCT GAAACTAAAA CCAACAGCGA CAAACTGCAA
    TTTGAATATA ATGAGGAAGC TTCAAAGCGA ATCAAAAAGA CAGATGGCAG CAGTATTAAC
    ACTAAAGGAA AGGTCTATGA CATGGAGTTG GGTGAGGAAT TCTATCTTCC ACAAAACAAA
    AAAGAGAGCA GGCAAATTGC ACCTGAATTG TCAGACCTTA TTATCTATTG CCAAGCTGTC
    AAATTCCCAG GCCTTACAAC ATTAAACCCA TGTGGATCTG GCAGAGGGAA GGAAAGAAAA
    AGCAGGAAGT CCATTTTTGG CAACAACCCT GGCAGAACAA GTCCTGGGGA ACCAACAGCA
    CTTGCCAAAA CATCTGGAAA AGGTACAAAT GAAACACGGC AGAGCTGGGA AGAGCCATGT
    TCTCCTCCCT TTAACCCCAG CACTTCCCTC AGTGCAATAA TCAGGACACC CAGGTGCTAC
    CATATCTCTT CCCTAAATGA GAACGCAGCC AAGCGCTTGT GCCGAAGGTA TTCTCAGAAA
    CTCATTCAAC ATACCGCATA TCAACTGCTC CGAACATATC CCGCCGCCAC TCGGATAGAC
    TCTTCCAACC CACATCCTCT TATTTTCTGG CTTCATGGTG TACAGTTGGT TGCTCTAAAC
    TACCAAACTG ATGATCTCCC CTTACATCTA AACGCCGCCA TGTTTGAAGC AAATGGTGGT
    TGCGGATATG TGTTAAAGCC CCCCGTGCAG TGGGATAGGA CCTGCCCAAT GTACCAATTA
    TTCTCACCAT TAGAACGAGA TTTAGAAAAT ATGGAACCAG CAATATATTC TTTGACGATA
    GTCTCTGGGC AAAATGTGTG TCCAGGTAAC AGCTCAGGCA GCCCCTGCAT TGAAATTGAT
    GTCTTGGGTA TGCCAGTAGA CAGCTGCCAT TTTCGGACCA AACCAATCCA TCGCAATACC
    TTAAATCCCA TGTGGAATGA ACAATTCTTA TTCCGAATAT ACTTTGAGGA TCTTGTGTTT
    CTTCGGTTTG CTGTGGTGGA AAACAACAGC TCTGCTGTAA CTGCCCAAAG AATAATTAAT
    CTTAAAGCTT TAAAGAGAGG TAACAGACAC CTTCAGCTAA GAAATTTACA TAATGAGCCC
    TTAGAGGTTT CTACCTTATT TATAAATAGC AGGAGAATGG AAGAAATCCC TAATGGAAAT
    ACCTTACCCG CCTCCTTGTT TTTCAGCTCA GAAGAAAGAA AGACTCCAGC CACATTTAAA
    GCTACAGTAC ATGGGATTCC TGGGCCAGAA CCATTTACAG TGTTTGAAAT CAGTCTTGGG
    ACAACTGCAA AGCAACTTCT TGATCATATT TTGGCTACTA TACAAGGCAA TGAAGCAGAC
    ATCACTGACT ATTTTTTGAT GGAGGAAAAA TGCTTTATAT CTAAAGACAA AAATGAATGC
    AAGAAATTGC CGTTCCAGAG AGTGATTAGC CCAGAAGAGG ATATCCTGCA AATTTTGAAT
    AGCTGGTTCC CAGAAGAAGG CTACGTGGGG AGAATTGTTC TTAAAACACG AGAGGAGAAC
    ATGAATGACA AAAATGTTCA AGAGGACAAA GAAGAAAAAG AGGTGACCGT GAGCTCCGAT
    GAGGAGACTT TCTTTGTCCA GGTCCATGAT GTTTCTCCTG AACAGCCAAG AACGGTTATC
    AAAGCTTTAC GATTCAGTAC AGCGCAGGAA GTTATCCAGC AGGCTTTATG CAAGGCTAAA
    TACTCATACA GCATCCTGAG CAACCCTAAT CCTAGTGATT ATGTGCTTCT GGAGGAAGTC
    ATCAAAGAAG CTTCAAATAA GAAGTCTTCT ACCTCCAAAA CAATTCAGAG GGTTCTCTTG
    GATCAAGAGT GTGTATTCCA GGCACAGAGC AAGTGGAAGG GAGCAGGGAA ATTCATTCTT
    AAACTGAAGG AACAAGTCCA GGCGGCAAGA GACGACAAGC GCAAAGGTCT GTCTATAGCC
    AGTGAACTGA AGAAGCTAGC AAAGTCCAGC CGGCAATCTC GGAACTTCAC TTTGTCACCT
    CAGATATTTT TATCAGAAGG TACCCAGAAT AGGGATGAAA AACCTGCATG CAGTTTGTCC
    TTCAGTGACA TCAATGAATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.