ORF » Species Summary » Danio rerio » MTHFR cDNA ORF clone

MTHFR cDNA ORF clone, Danio rerio(Zebrafish)

The following MTHFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTHFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa32223 NM_001128255.2
Latest version!		 Danio rerio methylenetetrahydrofolate reductase (NAD(P)H) (mthfr), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa32223 NM_001281840.1
Latest version!		 Danio rerio methylenetetrahydrofolate reductase (NAD(P)H) (mthfr), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa59975 XM_017357355.1
Latest version!		 Danio rerio methylenetetrahydrofolate reductase (NAD(P)H) (mthfr), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    MTHFR ( NM_001128255.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001121727.1 Danio rerio methylenetetrahydrofolate reductase (NAD(P)H) (mthfr), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID ODa32223
    Clone ID Related Accession (Same CDS sequence) NM_001128255.2 , NM_001281840.1
    Accession Version NM_001128255.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product methylenetetrahydrofolate reductase
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR792439.12. On Aug 8, 2013 this sequence version replaced NM_001128255.1. Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: JN848836.1, GFIL01027083.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACC AAAGAGCTGA CGTGGGTTGG CACTCTAAGA GTGATTCCAG CGGGGCCAGT 
    AACAGTGGAG AGAGCTCCAG AGAGAGCTCC AGATGCTCCA CCCCAGTACC GGACGCTGAC 
    CGCACCGACC GGCTACGGGA CAAAATGAAA CGACGTATCG AGTCAGGGGA TCACTGGTTC 
    TCTTTAGAGT TCTTCCCACC TCGCACATCG TCTGGAGCGG TCAATCTTAT CTCTAGATTT 
    GACCGAATGG GTTCCGGAGG CCCTCTCTTC ATTGACATCA CTTGGCATCC CGCTGGAGAT 
    CCGGGCTCAG ATAAAGAAAC GTCTTCCATG ATGATAGCAA GCACAGCGGT CAACTATTGC 
    GGTCTGGAGA GTGTCCTGCA TTTAACATGC TGCAATCAGA CAAAGGAGAA AATAACAGAC 
    CATTTAAATA AAGCCAAGCG GCTCGGCCTA AAGAATATCA TGGCCCTGAG AGGAGATCCT 
    GTTGGGGAAG ATTGGGTGGA GGAAGATGGT GGTTTTAACT TTGCGACAGA TCTGGTCAAA 
    CACATTCGCC ATGAATTCGA TGACTACTTT GATATCTGTG TAGCAGGTTA CCCCACGGGT 
    CACCCGGAGG CTGAGAGCTA TGAAGATGAC CTCCGGCACT TGAAGGAGAA GGTTGATGCT 
    GGAGCACACT TCATCGTCAC CCAGCTGTTT TTCAGAGCAG AAACCTTCCT TAAGTTTGTC 
    AAAGACTGTC GAGCCATTGG CATCACCTGC CCGATTCTTC CAGGAATCTT CCCCATCCAG 
    GGTTATCATT CACTCCGCCA GCTGGTCAAA CTCTCCAAAC TTGAAGTGCC GGAGGAGATC 
    AAGCAAGTGA TTGAGCCCAT TAAGGATAAT GATGCCGCTA TCCGGAACTA CGGCATCCAG 
    CAGGCTGTAG AGATGTGTAA AGTTCTGCTG GCCAGTGGGG AAGTCTCCGG CCTGCACTTT 
    TACACCTTAA ACCGAGAGGT GGCCACCATG GAAGTGCTCA GGCAGCTGGG ATTATGGGCA 
    GAGGATCCAC GGCGACCTCT GCCCTGGGCC ATCAGCGCCC ACCCAAAGCG TAAGGTAGAA 
    GACGTCAGGC CAATTTTCTG GGCTTCCCGA CCCAAAAGCT ACATCTACAG AACACAAGAC 
    TGGGATGATT TCCCTAACGG AAGATGGGGG AACTCTTCTT CACCAGCCTT TGGCGAGTTG 
    ACGGATTATT ACTTGTTCTA CCTGAAGAGT AAGTCTCCTA AAGAGGCGCT GCTGCAGATG 
    TGGGGAGAGG AGCTCATGAG CGAAGAGAGC GTTTATGAAG TCTTTACCAA CTACATCACT 
    GGACAAACCA ACCGCAGTGG ACACAAGGTG ATGTGTCTGC CATGGAACGA CGATCCTCTG 
    GCTCCAGAAA CCAACCTGCT GAAGGATGAG CTGGAGAAGG TGAACCGCAG AGGTGTTCTC 
    ACCATTAACT CTCAACCCAA CATCAATGGC AAAGCCTCTT CTGATGCGAT CGTGGGCTGG 
    GGTCCTGCAG GAGGATACGT CTTTCAAAAG GCGTATTTGG AGTTTTTCAC ATCAAGTGAA 
    AACGTTACTG CGCTTCTGCA AGTGCTGAAG AAATATGAAC CACGTGTGAA CTACCAAATA 
    GTCAATGTGC AGGGCAGGAA TATCACAAAC GCCCATGATA TGCAGCCCAA CGCTGTGACA 
    TGGGGGATTT TCCCAGGCAG AGAGATCATT CAGCCCACTG TGGTGGATCC AGTCAGCTTT 
    ATGTACTGGA AGGACGAGGC TTTCGCGTTG TGGATTGAAC AATGGGCGAA GCTATACGAG 
    GACGAATCGC CCTCGCGCAT GATCATTCAG TACATCCACG ACAACTACTT TCTGGTCAAT 
    CTGGTGGACA ACGACTTCCC GCTCGACAAC TGTCTGTGGC AGGTGATCGA AGACATGTTT 
    GCGCTCCTGG ACTCTTCTCA AGTACCGCAG CAGGAGGCAA ATGAATTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121727.1
    CDS92..2062
    Translation

    Target ORF information:

    RefSeq Version NM_001128255.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio methylenetetrahydrofolate reductase (NAD(P)H) (mthfr), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001128255.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACC AAAGAGCTGA CGTGGGTTGG CACTCTAAGA GTGATTCCAG CGGGGCCAGT 
    AACAGTGGAG AGAGCTCCAG AGAGAGCTCC AGATGCTCCA CCCCAGTACC GGACGCTGAC 
    CGCACCGACC GGCTACGGGA CAAAATGAAA CGACGTATCG AGTCAGGGGA TCACTGGTTC 
    TCTTTAGAGT TCTTCCCACC TCGCACATCG TCTGGAGCGG TCAATCTTAT CTCTAGATTT 
    GACCGAATGG GTTCCGGAGG CCCTCTCTTC ATTGACATCA CTTGGCATCC CGCTGGAGAT 
    CCGGGCTCAG ATAAAGAAAC GTCTTCCATG ATGATAGCAA GCACAGCGGT CAACTATTGC 
    GGTCTGGAGA GTGTCCTGCA TTTAACATGC TGCAATCAGA CAAAGGAGAA AATAACAGAC 
    CATTTAAATA AAGCCAAGCG GCTCGGCCTA AAGAATATCA TGGCCCTGAG AGGAGATCCT 
    GTTGGGGAAG ATTGGGTGGA GGAAGATGGT GGTTTTAACT TTGCGACAGA TCTGGTCAAA 
    CACATTCGCC ATGAATTCGA TGACTACTTT GATATCTGTG TAGCAGGTTA CCCCACGGGT 
    CACCCGGAGG CTGAGAGCTA TGAAGATGAC CTCCGGCACT TGAAGGAGAA GGTTGATGCT 
    GGAGCACACT TCATCGTCAC CCAGCTGTTT TTCAGAGCAG AAACCTTCCT TAAGTTTGTC 
    AAAGACTGTC GAGCCATTGG CATCACCTGC CCGATTCTTC CAGGAATCTT CCCCATCCAG 
    GGTTATCATT CACTCCGCCA GCTGGTCAAA CTCTCCAAAC TTGAAGTGCC GGAGGAGATC 
    AAGCAAGTGA TTGAGCCCAT TAAGGATAAT GATGCCGCTA TCCGGAACTA CGGCATCCAG 
    CAGGCTGTAG AGATGTGTAA AGTTCTGCTG GCCAGTGGGG AAGTCTCCGG CCTGCACTTT 
    TACACCTTAA ACCGAGAGGT GGCCACCATG GAAGTGCTCA GGCAGCTGGG ATTATGGGCA 
    GAGGATCCAC GGCGACCTCT GCCCTGGGCC ATCAGCGCCC ACCCAAAGCG TAAGGTAGAA 
    GACGTCAGGC CAATTTTCTG GGCTTCCCGA CCCAAAAGCT ACATCTACAG AACACAAGAC 
    TGGGATGATT TCCCTAACGG AAGATGGGGG AACTCTTCTT CACCAGCCTT TGGCGAGTTG 
    ACGGATTATT ACTTGTTCTA CCTGAAGAGT AAGTCTCCTA AAGAGGCGCT GCTGCAGATG 
    TGGGGAGAGG AGCTCATGAG CGAAGAGAGC GTTTATGAAG TCTTTACCAA CTACATCACT 
    GGACAAACCA ACCGCAGTGG ACACAAGGTG ATGTGTCTGC CATGGAACGA CGATCCTCTG 
    GCTCCAGAAA CCAACCTGCT GAAGGATGAG CTGGAGAAGG TGAACCGCAG AGGTGTTCTC 
    ACCATTAACT CTCAACCCAA CATCAATGGC AAAGCCTCTT CTGATGCGAT CGTGGGCTGG 
    GGTCCTGCAG GAGGATACGT CTTTCAAAAG GCGTATTTGG AGTTTTTCAC ATCAAGTGAA 
    AACGTTACTG CGCTTCTGCA AGTGCTGAAG AAATATGAAC CACGTGTGAA CTACCAAATA 
    GTCAATGTGC AGGGCAGGAA TATCACAAAC GCCCATGATA TGCAGCCCAA CGCTGTGACA 
    TGGGGGATTT TCCCAGGCAG AGAGATCATT CAGCCCACTG TGGTGGATCC AGTCAGCTTT 
    ATGTACTGGA AGGACGAGGC TTTCGCGTTG TGGATTGAAC AATGGGCGAA GCTATACGAG 
    GACGAATCGC CCTCGCGCAT GATCATTCAG TACATCCACG ACAACTACTT TCTGGTCAAT 
    CTGGTGGACA ACGACTTCCC GCTCGACAAC TGTCTGTGGC AGGTGATCGA AGACATGTTT 
    GCGCTCCTGG ACTCTTCTCA AGTACCGCAG CAGGAGGCAA ATGAATTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MTHFR ( NM_001281840.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001268769.1 Danio rerio methylenetetrahydrofolate reductase (NAD(P)H) (mthfr), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID ODa32223
    Clone ID Related Accession (Same CDS sequence) NM_001128255.2 , NM_001281840.1
    Accession Version NM_001281840.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product methylenetetrahydrofolate reductase
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR792439.12. On Aug 31, 2013 this sequence version replaced XM_005167283.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: GFIL01027083.1, GDQH01001715.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACC AAAGAGCTGA CGTGGGTTGG CACTCTAAGA GTGATTCCAG CGGGGCCAGT 
    AACAGTGGAG AGAGCTCCAG AGAGAGCTCC AGATGCTCCA CCCCAGTACC GGACGCTGAC 
    CGCACCGACC GGCTACGGGA CAAAATGAAA CGACGTATCG AGTCAGGGGA TCACTGGTTC 
    TCTTTAGAGT TCTTCCCACC TCGCACATCG TCTGGAGCGG TCAATCTTAT CTCTAGATTT 
    GACCGAATGG GTTCCGGAGG CCCTCTCTTC ATTGACATCA CTTGGCATCC CGCTGGAGAT 
    CCGGGCTCAG ATAAAGAAAC GTCTTCCATG ATGATAGCAA GCACAGCGGT CAACTATTGC 
    GGTCTGGAGA GTGTCCTGCA TTTAACATGC TGCAATCAGA CAAAGGAGAA AATAACAGAC 
    CATTTAAATA AAGCCAAGCG GCTCGGCCTA AAGAATATCA TGGCCCTGAG AGGAGATCCT 
    GTTGGGGAAG ATTGGGTGGA GGAAGATGGT GGTTTTAACT TTGCGACAGA TCTGGTCAAA 
    CACATTCGCC ATGAATTCGA TGACTACTTT GATATCTGTG TAGCAGGTTA CCCCACGGGT 
    CACCCGGAGG CTGAGAGCTA TGAAGATGAC CTCCGGCACT TGAAGGAGAA GGTTGATGCT 
    GGAGCACACT TCATCGTCAC CCAGCTGTTT TTCAGAGCAG AAACCTTCCT TAAGTTTGTC 
    AAAGACTGTC GAGCCATTGG CATCACCTGC CCGATTCTTC CAGGAATCTT CCCCATCCAG 
    GGTTATCATT CACTCCGCCA GCTGGTCAAA CTCTCCAAAC TTGAAGTGCC GGAGGAGATC 
    AAGCAAGTGA TTGAGCCCAT TAAGGATAAT GATGCCGCTA TCCGGAACTA CGGCATCCAG 
    CAGGCTGTAG AGATGTGTAA AGTTCTGCTG GCCAGTGGGG AAGTCTCCGG CCTGCACTTT 
    TACACCTTAA ACCGAGAGGT GGCCACCATG GAAGTGCTCA GGCAGCTGGG ATTATGGGCA 
    GAGGATCCAC GGCGACCTCT GCCCTGGGCC ATCAGCGCCC ACCCAAAGCG TAAGGTAGAA 
    GACGTCAGGC CAATTTTCTG GGCTTCCCGA CCCAAAAGCT ACATCTACAG AACACAAGAC 
    TGGGATGATT TCCCTAACGG AAGATGGGGG AACTCTTCTT CACCAGCCTT TGGCGAGTTG 
    ACGGATTATT ACTTGTTCTA CCTGAAGAGT AAGTCTCCTA AAGAGGCGCT GCTGCAGATG 
    TGGGGAGAGG AGCTCATGAG CGAAGAGAGC GTTTATGAAG TCTTTACCAA CTACATCACT 
    GGACAAACCA ACCGCAGTGG ACACAAGGTG ATGTGTCTGC CATGGAACGA CGATCCTCTG 
    GCTCCAGAAA CCAACCTGCT GAAGGATGAG CTGGAGAAGG TGAACCGCAG AGGTGTTCTC 
    ACCATTAACT CTCAACCCAA CATCAATGGC AAAGCCTCTT CTGATGCGAT CGTGGGCTGG 
    GGTCCTGCAG GAGGATACGT CTTTCAAAAG GCGTATTTGG AGTTTTTCAC ATCAAGTGAA 
    AACGTTACTG CGCTTCTGCA AGTGCTGAAG AAATATGAAC CACGTGTGAA CTACCAAATA 
    GTCAATGTGC AGGGCAGGAA TATCACAAAC GCCCATGATA TGCAGCCCAA CGCTGTGACA 
    TGGGGGATTT TCCCAGGCAG AGAGATCATT CAGCCCACTG TGGTGGATCC AGTCAGCTTT 
    ATGTACTGGA AGGACGAGGC TTTCGCGTTG TGGATTGAAC AATGGGCGAA GCTATACGAG 
    GACGAATCGC CCTCGCGCAT GATCATTCAG TACATCCACG ACAACTACTT TCTGGTCAAT 
    CTGGTGGACA ACGACTTCCC GCTCGACAAC TGTCTGTGGC AGGTGATCGA AGACATGTTT 
    GCGCTCCTGG ACTCTTCTCA AGTACCGCAG CAGGAGGCAA ATGAATTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001268769.1
    CDS97..2067
    Translation

    Target ORF information:

    RefSeq Version NM_001281840.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio methylenetetrahydrofolate reductase (NAD(P)H) (mthfr), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001281840.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACC AAAGAGCTGA CGTGGGTTGG CACTCTAAGA GTGATTCCAG CGGGGCCAGT 
    AACAGTGGAG AGAGCTCCAG AGAGAGCTCC AGATGCTCCA CCCCAGTACC GGACGCTGAC 
    CGCACCGACC GGCTACGGGA CAAAATGAAA CGACGTATCG AGTCAGGGGA TCACTGGTTC 
    TCTTTAGAGT TCTTCCCACC TCGCACATCG TCTGGAGCGG TCAATCTTAT CTCTAGATTT 
    GACCGAATGG GTTCCGGAGG CCCTCTCTTC ATTGACATCA CTTGGCATCC CGCTGGAGAT 
    CCGGGCTCAG ATAAAGAAAC GTCTTCCATG ATGATAGCAA GCACAGCGGT CAACTATTGC 
    GGTCTGGAGA GTGTCCTGCA TTTAACATGC TGCAATCAGA CAAAGGAGAA AATAACAGAC 
    CATTTAAATA AAGCCAAGCG GCTCGGCCTA AAGAATATCA TGGCCCTGAG AGGAGATCCT 
    GTTGGGGAAG ATTGGGTGGA GGAAGATGGT GGTTTTAACT TTGCGACAGA TCTGGTCAAA 
    CACATTCGCC ATGAATTCGA TGACTACTTT GATATCTGTG TAGCAGGTTA CCCCACGGGT 
    CACCCGGAGG CTGAGAGCTA TGAAGATGAC CTCCGGCACT TGAAGGAGAA GGTTGATGCT 
    GGAGCACACT TCATCGTCAC CCAGCTGTTT TTCAGAGCAG AAACCTTCCT TAAGTTTGTC 
    AAAGACTGTC GAGCCATTGG CATCACCTGC CCGATTCTTC CAGGAATCTT CCCCATCCAG 
    GGTTATCATT CACTCCGCCA GCTGGTCAAA CTCTCCAAAC TTGAAGTGCC GGAGGAGATC 
    AAGCAAGTGA TTGAGCCCAT TAAGGATAAT GATGCCGCTA TCCGGAACTA CGGCATCCAG 
    CAGGCTGTAG AGATGTGTAA AGTTCTGCTG GCCAGTGGGG AAGTCTCCGG CCTGCACTTT 
    TACACCTTAA ACCGAGAGGT GGCCACCATG GAAGTGCTCA GGCAGCTGGG ATTATGGGCA 
    GAGGATCCAC GGCGACCTCT GCCCTGGGCC ATCAGCGCCC ACCCAAAGCG TAAGGTAGAA 
    GACGTCAGGC CAATTTTCTG GGCTTCCCGA CCCAAAAGCT ACATCTACAG AACACAAGAC 
    TGGGATGATT TCCCTAACGG AAGATGGGGG AACTCTTCTT CACCAGCCTT TGGCGAGTTG 
    ACGGATTATT ACTTGTTCTA CCTGAAGAGT AAGTCTCCTA AAGAGGCGCT GCTGCAGATG 
    TGGGGAGAGG AGCTCATGAG CGAAGAGAGC GTTTATGAAG TCTTTACCAA CTACATCACT 
    GGACAAACCA ACCGCAGTGG ACACAAGGTG ATGTGTCTGC CATGGAACGA CGATCCTCTG 
    GCTCCAGAAA CCAACCTGCT GAAGGATGAG CTGGAGAAGG TGAACCGCAG AGGTGTTCTC 
    ACCATTAACT CTCAACCCAA CATCAATGGC AAAGCCTCTT CTGATGCGAT CGTGGGCTGG 
    GGTCCTGCAG GAGGATACGT CTTTCAAAAG GCGTATTTGG AGTTTTTCAC ATCAAGTGAA 
    AACGTTACTG CGCTTCTGCA AGTGCTGAAG AAATATGAAC CACGTGTGAA CTACCAAATA 
    GTCAATGTGC AGGGCAGGAA TATCACAAAC GCCCATGATA TGCAGCCCAA CGCTGTGACA 
    TGGGGGATTT TCCCAGGCAG AGAGATCATT CAGCCCACTG TGGTGGATCC AGTCAGCTTT 
    ATGTACTGGA AGGACGAGGC TTTCGCGTTG TGGATTGAAC AATGGGCGAA GCTATACGAG 
    GACGAATCGC CCTCGCGCAT GATCATTCAG TACATCCACG ACAACTACTT TCTGGTCAAT 
    CTGGTGGACA ACGACTTCCC GCTCGACAAC TGTCTGTGGC AGGTGATCGA AGACATGTTT 
    GCGCTCCTGG ACTCTTCTCA AGTACCGCAG CAGGAGGCAA ATGAATTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MTHFR ( XM_017357355.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212844.1 Danio rerio methylenetetrahydrofolate reductase (NAD(P)H) (mthfr), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa59975
    Clone ID Related Accession (Same CDS sequence) XM_017357355.1
    Accession Version XM_017357355.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1770bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product methylenetetrahydrofolate reductase isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGTGAACC AAAGAGCTGA CGTGGGTTGG CACTCTAAGA GTGATTCCAG CGGGGCCAGT 
    AACAGTGGAG AGAGCTCCAG AGAGAGCTCC AGATGCTCCA CCCCAGTACC GGACGCTGAC 
    CGCACCGACC GGCTACGGGA CAAAATGAAA CGACGTATCG AGTCAGGGGA TCACTGGTTC 
    TCTTTAGAGT TCTTCCCACC TCGCACATCG TCTGGAGCGG TCAATCTTAT CTCTAGATTT 
    GACCGAATGG GTTCCGGAGG CCCTCTCTTC ATTGACATCA CTTGGCATCC CGCTGGAGAT 
    CCGGGCTCAG ATAAAGAAAC GTCTTCCATG ATGATAGCAA GCACAGCGGT CAACTATTGC 
    GGTCTGGAGA GTGTCCTGCA TTTAACATGC TGCAATCAGA CAAAGGAGAA AATAACAGAC 
    CATTTAAATA AAGCCAAGCG GCTCGGCCTA AAGAATATCA TGGCCCTGAG AGGAGATCCT 
    GTTGGGGAAG ATTGGGTGGA GGAAGATGGT GGTTTTAACT TTGCGACAGA TCTGGTCAAA 
    CACATTCGCC ATGAATTCGA TGACTACTTT GATATCTGTG TAGCAGGTTA CCCCACGGGT 
    CACCCGGAGG CTGAGAGCTA TGAAGATGAC CTCCGGCACT TGAAGGAGAA GGTTGATGCT 
    GGAGCACACT TCATCGTCAC CCAGCTGTTT TTCAGAGCAG AAACCTTCCT TAAGTTTGTC 
    AAAGACTGTC GAGCCATTGG CATCACCTGC CCGATTCTTC CAGGAATCTT CCCCATCCAG 
    GGTTATCATT CACTCCGCCA GCTGGTCAAA CTCTCCAAAC TTGAAGTGCC GGAGGAGATC 
    AAGCAAGTGA TTGAGCCCAT TAAGGATAAT GATGCCGCTA TCCGGAACTA CGGCATCCAG 
    CAGGCTGTAG AGATGTGTAA AGTTCTGCTG GCCAGTGGGG AAGTCTCCGG CCTGCACTTT 
    TACACCTTAA ACCGAGAGGT GGCCACCATG GAAGTGCTCA GGCAGCTGGG ATTATGGGCA 
    GAGGATCCAC GGCGACCTCT GCCCTGGGCC ATCAGCGCCC ACCCAAAGCG TAAGGTAGAA 
    GACGTCAGGC CAATTTTCTG GGCTTCCCGA CCCAAAAGCT ACATCTACAG AACACAAGAC 
    TGGGATGATT TCCCTAACGG AAGATGGGGG AACTCTTCTT CACCAGCCTT TGGCGAGTTG 
    ACGGATTATT ACTTGTTCTA CCTGAAGAGT AAGTCTCCTA AAGAGGCGCT GCTGCAGATG 
    TGGGGAGAGG AGCTCATGAG CGAAGAGAGC GTTTATGAAG TCTTTACCAA CTACATCACT 
    GGACAAACCA ACCGCAGTGG ACACAAGGTG ATGTGTCTGC CATGGAACGA CGATCCTCTG 
    GCTCCAGAAA CCAACCTGCT GAAGGATGAG CTGGAGAAGG TGAACCGCAG AGGTGTTCTC 
    ACCATTAACT CTCAACCCAA CATCAATGGC AAAGCCTCTT CTGATGCGAT CGTGGGCTGG 
    GGTCCTGCAG GAGGATACGT CTTTCAAAAG GCGTATTTGG AGTTTTTCAC ATCAAGTGAA 
    AACGTTACTG CGCTTCTGCA AGTGCTGAAG AAATATGAAC CACGTGTGAA CTACCAAATA 
    GTCAATGTGC AGGGCAGGAA TATCACAAAC GCCCATGATA TGCAGCCCAA CGCTGTGACA 
    TGGGGGATTT TCCCAGGCAG AGAGATCATT CAGCCCACTG TGGTGGATCC AGTCAGCTTT 
    ATGTACTGGA AGGTGATCGT TCTTCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212844.1
    CDS210..1979
    Translation

    Target ORF information:

    RefSeq Version XM_017357355.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio methylenetetrahydrofolate reductase (NAD(P)H) (mthfr), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017357355.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGTGAACC AAAGAGCTGA CGTGGGTTGG CACTCTAAGA GTGATTCCAG CGGGGCCAGT 
    AACAGTGGAG AGAGCTCCAG AGAGAGCTCC AGATGCTCCA CCCCAGTACC GGACGCTGAC 
    CGCACCGACC GGCTACGGGA CAAAATGAAA CGACGTATCG AGTCAGGGGA TCACTGGTTC 
    TCTTTAGAGT TCTTCCCACC TCGCACATCG TCTGGAGCGG TCAATCTTAT CTCTAGATTT 
    GACCGAATGG GTTCCGGAGG CCCTCTCTTC ATTGACATCA CTTGGCATCC CGCTGGAGAT 
    CCGGGCTCAG ATAAAGAAAC GTCTTCCATG ATGATAGCAA GCACAGCGGT CAACTATTGC 
    GGTCTGGAGA GTGTCCTGCA TTTAACATGC TGCAATCAGA CAAAGGAGAA AATAACAGAC 
    CATTTAAATA AAGCCAAGCG GCTCGGCCTA AAGAATATCA TGGCCCTGAG AGGAGATCCT 
    GTTGGGGAAG ATTGGGTGGA GGAAGATGGT GGTTTTAACT TTGCGACAGA TCTGGTCAAA 
    CACATTCGCC ATGAATTCGA TGACTACTTT GATATCTGTG TAGCAGGTTA CCCCACGGGT 
    CACCCGGAGG CTGAGAGCTA TGAAGATGAC CTCCGGCACT TGAAGGAGAA GGTTGATGCT 
    GGAGCACACT TCATCGTCAC CCAGCTGTTT TTCAGAGCAG AAACCTTCCT TAAGTTTGTC 
    AAAGACTGTC GAGCCATTGG CATCACCTGC CCGATTCTTC CAGGAATCTT CCCCATCCAG 
    GGTTATCATT CACTCCGCCA GCTGGTCAAA CTCTCCAAAC TTGAAGTGCC GGAGGAGATC 
    AAGCAAGTGA TTGAGCCCAT TAAGGATAAT GATGCCGCTA TCCGGAACTA CGGCATCCAG 
    CAGGCTGTAG AGATGTGTAA AGTTCTGCTG GCCAGTGGGG AAGTCTCCGG CCTGCACTTT 
    TACACCTTAA ACCGAGAGGT GGCCACCATG GAAGTGCTCA GGCAGCTGGG ATTATGGGCA 
    GAGGATCCAC GGCGACCTCT GCCCTGGGCC ATCAGCGCCC ACCCAAAGCG TAAGGTAGAA 
    GACGTCAGGC CAATTTTCTG GGCTTCCCGA CCCAAAAGCT ACATCTACAG AACACAAGAC 
    TGGGATGATT TCCCTAACGG AAGATGGGGG AACTCTTCTT CACCAGCCTT TGGCGAGTTG 
    ACGGATTATT ACTTGTTCTA CCTGAAGAGT AAGTCTCCTA AAGAGGCGCT GCTGCAGATG 
    TGGGGAGAGG AGCTCATGAG CGAAGAGAGC GTTTATGAAG TCTTTACCAA CTACATCACT 
    GGACAAACCA ACCGCAGTGG ACACAAGGTG ATGTGTCTGC CATGGAACGA CGATCCTCTG 
    GCTCCAGAAA CCAACCTGCT GAAGGATGAG CTGGAGAAGG TGAACCGCAG AGGTGTTCTC 
    ACCATTAACT CTCAACCCAA CATCAATGGC AAAGCCTCTT CTGATGCGAT CGTGGGCTGG 
    GGTCCTGCAG GAGGATACGT CTTTCAAAAG GCGTATTTGG AGTTTTTCAC ATCAAGTGAA 
    AACGTTACTG CGCTTCTGCA AGTGCTGAAG AAATATGAAC CACGTGTGAA CTACCAAATA 
    GTCAATGTGC AGGGCAGGAA TATCACAAAC GCCCATGATA TGCAGCCCAA CGCTGTGACA 
    TGGGGGATTT TCCCAGGCAG AGAGATCATT CAGCCCACTG TGGTGGATCC AGTCAGCTTT 
    ATGTACTGGA AGGTGATCGT TCTTCCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

見積依頼

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