MTHFR gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_005617992.3	XP_005618049.1	methylenetetrahydrofolate reductase isoform X1
XM_014111461.2	XP_013966936.1	methylenetetrahydrofolate reductase isoform X3
XM_014111460.1	XP_013966935.1	methylenetetrahydrofolate reductase isoform X3
XM_014111461.1	XP_013966936.1	methylenetetrahydrofolate reductase isoform X3
XM_005617993.3	XP_005618050.1	methylenetetrahydrofolate reductase isoform X2
XM_014111464.1	XP_013966939.1	methylenetetrahydrofolate reductase isoform X4
XM_005617993.2	XP_005618050.1	methylenetetrahydrofolate reductase isoform X2
XM_005617992.2	XP_005618049.1	methylenetetrahydrofolate reductase isoform X1
XM_014111464.2	XP_013966939.1	methylenetetrahydrofolate reductase isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe03866	XM_005617993.3 Latest version!	Canis lupus familiaris methylenetetrahydrofolate reductase (MTHFR), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe03864	XM_005617992.3 Latest version!	Canis lupus familiaris methylenetetrahydrofolate reductase (MTHFR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe35535	XM_014111460.1 Latest version!	Canis lupus familiaris methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe35535	XM_014111461.2 Latest version!	Canis lupus familiaris methylenetetrahydrofolate reductase (MTHFR), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe35536	XM_014111464.2 Latest version!	Canis lupus familiaris methylenetetrahydrofolate reductase (MTHFR), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OCe35535	XM_014111461.1	Canis lupus familiaris methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe03866	XM_005617993.2	Canis lupus familiaris methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe03864	XM_005617992.2	Canis lupus familiaris methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe35536	XM_014111464.1	Canis lupus familiaris methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

MTHFR ( XM_005617993.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618050.1 Canis lupus familiaris methylenetetrahydrofolate reductase (MTHFR), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe03866
Clone ID Related Accession (Same CDS sequence)	XM_005617993.3 , XM_005617993.2
Accession Version	XM_005617993.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	methylenetetrahydrofolate reductase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617993.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGG AGCCCAGAGG GGACGGGGAC GGCGGTCCCA CTCCCCGCTC GGAGGGCAGC 
AGCAGCGGCA GCGAGAGCTC CAAGGATAGT TCGAGATGTT CCACGCCGGG GCTGGACCCT 
GAGCGCCACG AGAGACTCAG GGAGAAGATG AGGCGGAGGA TGGAATCCGG TGACAGGTGG 
TTCTCCTTGG AGTTCTTTCC TCCTCGGACT GCTGAGGGAG CGGTCAACCT CATCTCGAGG 
TTTGATCGGA TGGGGGCAGG TGGCCCCCTC TTCATAGATG TGACCTGGCA CCCGGCAGGG 
GACCCTGGCT CAGACAAGGA GACGTCCTCC ATGATGATTG CCAGCACCGC CGTGAACTAC 
TGTGGCCTGG AGACCATCCT GCACATGACC TGCTGCTGTC AGAGCCGGGA GCAGATCACA 
GGCTATCTGC ACAAGGCCAA GCGGCTGGGC CTGAAGAACA TCTTGGCGCT GAGGGGAGAC 
CCCGTGGGCG ACCAGTGGGA AGTTGTGGAA GGAGGCTTCA GCTATGCCGC GGACTTGGTG 
AGGCACATCC GAAGCGAGTT TGGGGACTAC TTTGACATCT GTGTGGCAGG TTACCCCAAA 
GGCCACCCCG ACGCAGGGAG CTTTGAGGCC GACCTGAAGT ACTTGAAGGA GAAGGTATCC 
GCAGGAGCCG ACTTCATCAT CACCCAGCTT TTCTTTGAGG CTGACACATT CTTCCACTTT 
GTTAAGGCTT GCAGCGAGAT AGGCATAACC TGCCCCATCC TCCCCGGAAT CTTTCCTATT 
CAGGGCTACC ACTCCCTTCG GCAGCTCGTG AAACTGTCCA AGCTGGAGGT ACCGCAGCAG 
ATCAAAGATG TGATTGAGCC AATCAAAGAC AATGATGCTG CGATCCGTAA CTATGGCATC 
GAGCAGGCTG TGAGCCTGTG CCAGGAGCTT CTGACCAGTG GCTTGGTGCC AGGCCTCCAC 
TTCTACACCC TCAACCGCGA GATGGCCACC ACAGAGGTGC TGAAGCGCCT GGGCATGTGG 
ATCGAGGACC CCAGGCGTCC CCTACCCTGG GCTGTCAGTG CCCACCCCAA GCGCAGGGAG 
GAAGATGTAC GCCCCATTTT CTGGGCCTCC AGACCAAAGA GTTACATCTA CCGTACACAA 
GAGTGGGATG AGTTCCCCAA TGGCCGCTGG GGCAACTCCT CCTCTCCAGC CTTCGGGGAG 
CTGAAGGACT ACTACCTCTT CTACCTGAAG AGCAAGTCCC CGAGGGAGGA GCTACTGAAG 
ATGTGGGGGG AGGAGCTGAC CAGTGAAGAA AGCGTCTTTG AAGTCTTTGC CCGCTACCTC 
TCGGGAGAGC CCAACCAGCA CGGCTACAAG GTGACTTGCC TGCCCTGGAA TGACGAGCCC 
TTGGCGGCCG AGACCAGCCT GATGAAGGAG GAGCTGCTGC GAGTGAACCG GCAGGGCATC 
CTCACCATCA ACTCCCAGCC CAACATCAAC GGGAAACCAT CCTCTGACCC CATCGTGGGC 
TGGGGCCCCA GCGGGGGCTA TGTCTTCCAG AAGGCCTACT TGGAGTTCTT CACTTCCCGC 
GAGACGGTGG GGGCGCTTCT GCAGGTGCTG AAGAAGTATG AGCTCCGGGT TAATTACCAC 
ATCGTGGACG TGAAGGGTGA AAACATCACC AATGCGCCTG AGCTGCAGCC CAATGCGGTC 
ACTTGGGGCA TCTTCCCGGG GCGGGAGATC ATCCAGCCTA CGGTGGTAGA CCCTGTCAGC 
TTCATGTTCT GGAAGGATGA AGCCTTCGCC CTGTGGATCG AGCAGTGGGG CAAGCTGTAT 
GAGGAGGAGT CCCCGTCCCG CATGATCATC CAGTACATCC ACGACAACTA CTTCCTGGTC 
AACCTGGTGG ACAATGAGTT TCCGCTGGAC AACTGCCTGT GGCAAGTGGT GGAAGACACG 
TTTGAACTTC TCAAGGGGCC CTCACCAGAG CGAGAGGCAG AGGCCGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618050.1
CDS	133..2103
Translation	MVKEPRGDGDGGPTPRSEGSSSGSESSKDSSRCSTPGLDPERHERLREKMRRRMESGDRWFSLEFFPPRTAEGAVNLISRFDRMGAGGPLFIDVTWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCCQSREQITGYLHKAKRLGLKNILALRGDPVGDQWEVVEGGFSYAADLVRHIRSEFGDYFDICVAGYPKGHPDAGSFEADLKYLKEKVSAGADFIITQLFFEADTFFHFVKACSEIGITCPILPGIFPIQGYHSLRQLVKLSKLEVPQQIKDVIEPIKDNDAAIRNYGIEQAVSLCQELLTSGLVPGLHFYTLNREMATTEVLKRLGMWIEDPRRPLPWAVSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFARYLSGEPNQHGYKVTCLPWNDEPLAAETSLMKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETVGALLQVLKKYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDNCLWQVVEDTFELLKGPSPEREAEAV

Target ORF information:

RefSeq Version	XM_005617993.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris methylenetetrahydrofolate reductase (MTHFR), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617993.3

ORF Insert Sequence:

ATGGTGAAGG AGCCCAGAGG GGACGGGGAC GGCGGTCCCA CTCCCCGCTC GGAGGGCAGC 
AGCAGCGGCA GCGAGAGCTC CAAGGATAGT TCGAGATGTT CCACGCCGGG GCTGGACCCT 
GAGCGCCACG AGAGACTCAG GGAGAAGATG AGGCGGAGGA TGGAATCCGG TGACAGGTGG 
TTCTCCTTGG AGTTCTTTCC TCCTCGGACT GCTGAGGGAG CGGTCAACCT CATCTCGAGG 
TTTGATCGGA TGGGGGCAGG TGGCCCCCTC TTCATAGATG TGACCTGGCA CCCGGCAGGG 
GACCCTGGCT CAGACAAGGA GACGTCCTCC ATGATGATTG CCAGCACCGC CGTGAACTAC 
TGTGGCCTGG AGACCATCCT GCACATGACC TGCTGCTGTC AGAGCCGGGA GCAGATCACA 
GGCTATCTGC ACAAGGCCAA GCGGCTGGGC CTGAAGAACA TCTTGGCGCT GAGGGGAGAC 
CCCGTGGGCG ACCAGTGGGA AGTTGTGGAA GGAGGCTTCA GCTATGCCGC GGACTTGGTG 
AGGCACATCC GAAGCGAGTT TGGGGACTAC TTTGACATCT GTGTGGCAGG TTACCCCAAA 
GGCCACCCCG ACGCAGGGAG CTTTGAGGCC GACCTGAAGT ACTTGAAGGA GAAGGTATCC 
GCAGGAGCCG ACTTCATCAT CACCCAGCTT TTCTTTGAGG CTGACACATT CTTCCACTTT 
GTTAAGGCTT GCAGCGAGAT AGGCATAACC TGCCCCATCC TCCCCGGAAT CTTTCCTATT 
CAGGGCTACC ACTCCCTTCG GCAGCTCGTG AAACTGTCCA AGCTGGAGGT ACCGCAGCAG 
ATCAAAGATG TGATTGAGCC AATCAAAGAC AATGATGCTG CGATCCGTAA CTATGGCATC 
GAGCAGGCTG TGAGCCTGTG CCAGGAGCTT CTGACCAGTG GCTTGGTGCC AGGCCTCCAC 
TTCTACACCC TCAACCGCGA GATGGCCACC ACAGAGGTGC TGAAGCGCCT GGGCATGTGG 
ATCGAGGACC CCAGGCGTCC CCTACCCTGG GCTGTCAGTG CCCACCCCAA GCGCAGGGAG 
GAAGATGTAC GCCCCATTTT CTGGGCCTCC AGACCAAAGA GTTACATCTA CCGTACACAA 
GAGTGGGATG AGTTCCCCAA TGGCCGCTGG GGCAACTCCT CCTCTCCAGC CTTCGGGGAG 
CTGAAGGACT ACTACCTCTT CTACCTGAAG AGCAAGTCCC CGAGGGAGGA GCTACTGAAG 
ATGTGGGGGG AGGAGCTGAC CAGTGAAGAA AGCGTCTTTG AAGTCTTTGC CCGCTACCTC 
TCGGGAGAGC CCAACCAGCA CGGCTACAAG GTGACTTGCC TGCCCTGGAA TGACGAGCCC 
TTGGCGGCCG AGACCAGCCT GATGAAGGAG GAGCTGCTGC GAGTGAACCG GCAGGGCATC 
CTCACCATCA ACTCCCAGCC CAACATCAAC GGGAAACCAT CCTCTGACCC CATCGTGGGC 
TGGGGCCCCA GCGGGGGCTA TGTCTTCCAG AAGGCCTACT TGGAGTTCTT CACTTCCCGC 
GAGACGGTGG GGGCGCTTCT GCAGGTGCTG AAGAAGTATG AGCTCCGGGT TAATTACCAC 
ATCGTGGACG TGAAGGGTGA AAACATCACC AATGCGCCTG AGCTGCAGCC CAATGCGGTC 
ACTTGGGGCA TCTTCCCGGG GCGGGAGATC ATCCAGCCTA CGGTGGTAGA CCCTGTCAGC 
TTCATGTTCT GGAAGGATGA AGCCTTCGCC CTGTGGATCG AGCAGTGGGG CAAGCTGTAT 
GAGGAGGAGT CCCCGTCCCG CATGATCATC CAGTACATCC ACGACAACTA CTTCCTGGTC 
AACCTGGTGG ACAATGAGTT TCCGCTGGAC AACTGCCTGT GGCAAGTGGT GGAAGACACG 
TTTGAACTTC TCAAGGGGCC CTCACCAGAG CGAGAGGCAG AGGCCGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFR ( XM_005617993.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618050.1 Canis lupus familiaris methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe03866
Clone ID Related Accession (Same CDS sequence)	XM_005617993.3 , XM_005617993.2
Accession Version	XM_005617993.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	methylenetetrahydrofolate reductase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726051.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617993.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGG AGCCCAGAGG GGACGGGGAC GGCGGTCCCA CTCCCCGCTC GGAGGGCAGC 
AGCAGCGGCA GCGAGAGCTC CAAGGATAGT TCGAGATGTT CCACGCCGGG GCTGGACCCT 
GAGCGCCACG AGAGACTCAG GGAGAAGATG AGGCGGAGGA TGGAATCCGG TGACAGGTGG 
TTCTCCTTGG AGTTCTTTCC TCCTCGGACT GCTGAGGGAG CGGTCAACCT CATCTCGAGG 
TTTGATCGGA TGGGGGCAGG TGGCCCCCTC TTCATAGATG TGACCTGGCA CCCGGCAGGG 
GACCCTGGCT CAGACAAGGA GACGTCCTCC ATGATGATTG CCAGCACCGC CGTGAACTAC 
TGTGGCCTGG AGACCATCCT GCACATGACC TGCTGCTGTC AGAGCCGGGA GCAGATCACA 
GGCTATCTGC ACAAGGCCAA GCGGCTGGGC CTGAAGAACA TCTTGGCGCT GAGGGGAGAC 
CCCGTGGGCG ACCAGTGGGA AGTTGTGGAA GGAGGCTTCA GCTATGCCGC GGACTTGGTG 
AGGCACATCC GAAGCGAGTT TGGGGACTAC TTTGACATCT GTGTGGCAGG TTACCCCAAA 
GGCCACCCCG ACGCAGGGAG CTTTGAGGCC GACCTGAAGT ACTTGAAGGA GAAGGTATCC 
GCAGGAGCCG ACTTCATCAT CACCCAGCTT TTCTTTGAGG CTGACACATT CTTCCACTTT 
GTTAAGGCTT GCAGCGAGAT AGGCATAACC TGCCCCATCC TCCCCGGAAT CTTTCCTATT 
CAGGGCTACC ACTCCCTTCG GCAGCTCGTG AAACTGTCCA AGCTGGAGGT ACCGCAGCAG 
ATCAAAGATG TGATTGAGCC AATCAAAGAC AATGATGCTG CGATCCGTAA CTATGGCATC 
GAGCAGGCTG TGAGCCTGTG CCAGGAGCTT CTGACCAGTG GCTTGGTGCC AGGCCTCCAC 
TTCTACACCC TCAACCGCGA GATGGCCACC ACAGAGGTGC TGAAGCGCCT GGGCATGTGG 
ATCGAGGACC CCAGGCGTCC CCTACCCTGG GCTGTCAGTG CCCACCCCAA GCGCAGGGAG 
GAAGATGTAC GCCCCATTTT CTGGGCCTCC AGACCAAAGA GTTACATCTA CCGTACACAA 
GAGTGGGATG AGTTCCCCAA TGGCCGCTGG GGCAACTCCT CCTCTCCAGC CTTCGGGGAG 
CTGAAGGACT ACTACCTCTT CTACCTGAAG AGCAAGTCCC CGAGGGAGGA GCTACTGAAG 
ATGTGGGGGG AGGAGCTGAC CAGTGAAGAA AGCGTCTTTG AAGTCTTTGC CCGCTACCTC 
TCGGGAGAGC CCAACCAGCA CGGCTACAAG GTGACTTGCC TGCCCTGGAA TGACGAGCCC 
TTGGCGGCCG AGACCAGCCT GATGAAGGAG GAGCTGCTGC GAGTGAACCG GCAGGGCATC 
CTCACCATCA ACTCCCAGCC CAACATCAAC GGGAAACCAT CCTCTGACCC CATCGTGGGC 
TGGGGCCCCA GCGGGGGCTA TGTCTTCCAG AAGGCCTACT TGGAGTTCTT CACTTCCCGC 
GAGACGGTGG GGGCGCTTCT GCAGGTGCTG AAGAAGTATG AGCTCCGGGT TAATTACCAC 
ATCGTGGACG TGAAGGGTGA AAACATCACC AATGCGCCTG AGCTGCAGCC CAATGCGGTC 
ACTTGGGGCA TCTTCCCGGG GCGGGAGATC ATCCAGCCTA CGGTGGTAGA CCCTGTCAGC 
TTCATGTTCT GGAAGGATGA AGCCTTCGCC CTGTGGATCG AGCAGTGGGG CAAGCTGTAT 
GAGGAGGAGT CCCCGTCCCG CATGATCATC CAGTACATCC ACGACAACTA CTTCCTGGTC 
AACCTGGTGG ACAATGAGTT TCCGCTGGAC AACTGCCTGT GGCAAGTGGT GGAAGACACG 
TTTGAACTTC TCAAGGGGCC CTCACCAGAG CGAGAGGCAG AGGCCGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618050.1
CDS	134..2104
Translation	MVKEPRGDGDGGPTPRSEGSSSGSESSKDSSRCSTPGLDPERHERLREKMRRRMESGDRWFSLEFFPPRTAEGAVNLISRFDRMGAGGPLFIDVTWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCCQSREQITGYLHKAKRLGLKNILALRGDPVGDQWEVVEGGFSYAADLVRHIRSEFGDYFDICVAGYPKGHPDAGSFEADLKYLKEKVSAGADFIITQLFFEADTFFHFVKACSEIGITCPILPGIFPIQGYHSLRQLVKLSKLEVPQQIKDVIEPIKDNDAAIRNYGIEQAVSLCQELLTSGLVPGLHFYTLNREMATTEVLKRLGMWIEDPRRPLPWAVSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFARYLSGEPNQHGYKVTCLPWNDEPLAAETSLMKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETVGALLQVLKKYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDNCLWQVVEDTFELLKGPSPEREAEAV

Target ORF information:

RefSeq Version	XM_005617993.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617993.2

ORF Insert Sequence:

ATGGTGAAGG AGCCCAGAGG GGACGGGGAC GGCGGTCCCA CTCCCCGCTC GGAGGGCAGC 
AGCAGCGGCA GCGAGAGCTC CAAGGATAGT TCGAGATGTT CCACGCCGGG GCTGGACCCT 
GAGCGCCACG AGAGACTCAG GGAGAAGATG AGGCGGAGGA TGGAATCCGG TGACAGGTGG 
TTCTCCTTGG AGTTCTTTCC TCCTCGGACT GCTGAGGGAG CGGTCAACCT CATCTCGAGG 
TTTGATCGGA TGGGGGCAGG TGGCCCCCTC TTCATAGATG TGACCTGGCA CCCGGCAGGG 
GACCCTGGCT CAGACAAGGA GACGTCCTCC ATGATGATTG CCAGCACCGC CGTGAACTAC 
TGTGGCCTGG AGACCATCCT GCACATGACC TGCTGCTGTC AGAGCCGGGA GCAGATCACA 
GGCTATCTGC ACAAGGCCAA GCGGCTGGGC CTGAAGAACA TCTTGGCGCT GAGGGGAGAC 
CCCGTGGGCG ACCAGTGGGA AGTTGTGGAA GGAGGCTTCA GCTATGCCGC GGACTTGGTG 
AGGCACATCC GAAGCGAGTT TGGGGACTAC TTTGACATCT GTGTGGCAGG TTACCCCAAA 
GGCCACCCCG ACGCAGGGAG CTTTGAGGCC GACCTGAAGT ACTTGAAGGA GAAGGTATCC 
GCAGGAGCCG ACTTCATCAT CACCCAGCTT TTCTTTGAGG CTGACACATT CTTCCACTTT 
GTTAAGGCTT GCAGCGAGAT AGGCATAACC TGCCCCATCC TCCCCGGAAT CTTTCCTATT 
CAGGGCTACC ACTCCCTTCG GCAGCTCGTG AAACTGTCCA AGCTGGAGGT ACCGCAGCAG 
ATCAAAGATG TGATTGAGCC AATCAAAGAC AATGATGCTG CGATCCGTAA CTATGGCATC 
GAGCAGGCTG TGAGCCTGTG CCAGGAGCTT CTGACCAGTG GCTTGGTGCC AGGCCTCCAC 
TTCTACACCC TCAACCGCGA GATGGCCACC ACAGAGGTGC TGAAGCGCCT GGGCATGTGG 
ATCGAGGACC CCAGGCGTCC CCTACCCTGG GCTGTCAGTG CCCACCCCAA GCGCAGGGAG 
GAAGATGTAC GCCCCATTTT CTGGGCCTCC AGACCAAAGA GTTACATCTA CCGTACACAA 
GAGTGGGATG AGTTCCCCAA TGGCCGCTGG GGCAACTCCT CCTCTCCAGC CTTCGGGGAG 
CTGAAGGACT ACTACCTCTT CTACCTGAAG AGCAAGTCCC CGAGGGAGGA GCTACTGAAG 
ATGTGGGGGG AGGAGCTGAC CAGTGAAGAA AGCGTCTTTG AAGTCTTTGC CCGCTACCTC 
TCGGGAGAGC CCAACCAGCA CGGCTACAAG GTGACTTGCC TGCCCTGGAA TGACGAGCCC 
TTGGCGGCCG AGACCAGCCT GATGAAGGAG GAGCTGCTGC GAGTGAACCG GCAGGGCATC 
CTCACCATCA ACTCCCAGCC CAACATCAAC GGGAAACCAT CCTCTGACCC CATCGTGGGC 
TGGGGCCCCA GCGGGGGCTA TGTCTTCCAG AAGGCCTACT TGGAGTTCTT CACTTCCCGC 
GAGACGGTGG GGGCGCTTCT GCAGGTGCTG AAGAAGTATG AGCTCCGGGT TAATTACCAC 
ATCGTGGACG TGAAGGGTGA AAACATCACC AATGCGCCTG AGCTGCAGCC CAATGCGGTC 
ACTTGGGGCA TCTTCCCGGG GCGGGAGATC ATCCAGCCTA CGGTGGTAGA CCCTGTCAGC 
TTCATGTTCT GGAAGGATGA AGCCTTCGCC CTGTGGATCG AGCAGTGGGG CAAGCTGTAT 
GAGGAGGAGT CCCCGTCCCG CATGATCATC CAGTACATCC ACGACAACTA CTTCCTGGTC 
AACCTGGTGG ACAATGAGTT TCCGCTGGAC AACTGCCTGT GGCAAGTGGT GGAAGACACG 
TTTGAACTTC TCAAGGGGCC CTCACCAGAG CGAGAGGCAG AGGCCGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFR ( XM_005617992.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618049.1 Canis lupus familiaris methylenetetrahydrofolate reductase (MTHFR), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe03864
Clone ID Related Accession (Same CDS sequence)	XM_005617992.3 , XM_005617992.2
Accession Version	XM_005617992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	methylenetetrahydrofolate reductase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617992.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGC CACCACCTGG GGTTCTCCCA GCCACAGTCC GCTGCTCCCG CCTAGGACTG 
TGGACTCTGG AGGCCGGCAG GGTCGGGTTC TCAGTGCCAC CCTCCATCAG CAGGAGCCCG 
GCCATGGTGA AGGAGCCCAG AGGGGACGGG GACGGCGGTC CCACTCCCCG CTCGGAGGGC 
AGCAGCAGCG GCAGCGAGAG CTCCAAGGAT AGTTCGAGAT GTTCCACGCC GGGGCTGGAC 
CCTGAGCGCC ACGAGAGACT CAGGGAGAAG ATGAGGCGGA GGATGGAATC CGGTGACAGG 
TGGTTCTCCT TGGAGTTCTT TCCTCCTCGG ACTGCTGAGG GAGCGGTCAA CCTCATCTCG 
AGGTTTGATC GGATGGGGGC AGGTGGCCCC CTCTTCATAG ATGTGACCTG GCACCCGGCA 
GGGGACCCTG GCTCAGACAA GGAGACGTCC TCCATGATGA TTGCCAGCAC CGCCGTGAAC 
TACTGTGGCC TGGAGACCAT CCTGCACATG ACCTGCTGCT GTCAGAGCCG GGAGCAGATC 
ACAGGCTATC TGCACAAGGC CAAGCGGCTG GGCCTGAAGA ACATCTTGGC GCTGAGGGGA 
GACCCCGTGG GCGACCAGTG GGAAGTTGTG GAAGGAGGCT TCAGCTATGC CGCGGACTTG 
GTGAGGCACA TCCGAAGCGA GTTTGGGGAC TACTTTGACA TCTGTGTGGC AGGTTACCCC 
AAAGGCCACC CCGACGCAGG GAGCTTTGAG GCCGACCTGA AGTACTTGAA GGAGAAGGTA 
TCCGCAGGAG CCGACTTCAT CATCACCCAG CTTTTCTTTG AGGCTGACAC ATTCTTCCAC 
TTTGTTAAGG CTTGCAGCGA GATAGGCATA ACCTGCCCCA TCCTCCCCGG AATCTTTCCT 
ATTCAGGGCT ACCACTCCCT TCGGCAGCTC GTGAAACTGT CCAAGCTGGA GGTACCGCAG 
CAGATCAAAG ATGTGATTGA GCCAATCAAA GACAATGATG CTGCGATCCG TAACTATGGC 
ATCGAGCAGG CTGTGAGCCT GTGCCAGGAG CTTCTGACCA GTGGCTTGGT GCCAGGCCTC 
CACTTCTACA CCCTCAACCG CGAGATGGCC ACCACAGAGG TGCTGAAGCG CCTGGGCATG 
TGGATCGAGG ACCCCAGGCG TCCCCTACCC TGGGCTGTCA GTGCCCACCC CAAGCGCAGG 
GAGGAAGATG TACGCCCCAT TTTCTGGGCC TCCAGACCAA AGAGTTACAT CTACCGTACA 
CAAGAGTGGG ATGAGTTCCC CAATGGCCGC TGGGGCAACT CCTCCTCTCC AGCCTTCGGG 
GAGCTGAAGG ACTACTACCT CTTCTACCTG AAGAGCAAGT CCCCGAGGGA GGAGCTACTG 
AAGATGTGGG GGGAGGAGCT GACCAGTGAA GAAAGCGTCT TTGAAGTCTT TGCCCGCTAC 
CTCTCGGGAG AGCCCAACCA GCACGGCTAC AAGGTGACTT GCCTGCCCTG GAATGACGAG 
CCCTTGGCGG CCGAGACCAG CCTGATGAAG GAGGAGCTGC TGCGAGTGAA CCGGCAGGGC 
ATCCTCACCA TCAACTCCCA GCCCAACATC AACGGGAAAC CATCCTCTGA CCCCATCGTG 
GGCTGGGGCC CCAGCGGGGG CTATGTCTTC CAGAAGGCCT ACTTGGAGTT CTTCACTTCC 
CGCGAGACGG TGGGGGCGCT TCTGCAGGTG CTGAAGAAGT ATGAGCTCCG GGTTAATTAC 
CACATCGTGG ACGTGAAGGG TGAAAACATC ACCAATGCGC CTGAGCTGCA GCCCAATGCG 
GTCACTTGGG GCATCTTCCC GGGGCGGGAG ATCATCCAGC CTACGGTGGT AGACCCTGTC 
AGCTTCATGT TCTGGAAGGA TGAAGCCTTC GCCCTGTGGA TCGAGCAGTG GGGCAAGCTG 
TATGAGGAGG AGTCCCCGTC CCGCATGATC ATCCAGTACA TCCACGACAA CTACTTCCTG 
GTCAACCTGG TGGACAATGA GTTTCCGCTG GACAACTGCC TGTGGCAAGT GGTGGAAGAC 
ACGTTTGAAC TTCTCAAGGG GCCCTCACCA GAGCGAGAGG CAGAGGCCGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618049.1
CDS	228..2321
Translation	MDQPPPGVLPATVRCSRLGLWTLEAGRVGFSVPPSISRSPAMVKEPRGDGDGGPTPRSEGSSSGSESSKDSSRCSTPGLDPERHERLREKMRRRMESGDRWFSLEFFPPRTAEGAVNLISRFDRMGAGGPLFIDVTWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCCQSREQITGYLHKAKRLGLKNILALRGDPVGDQWEVVEGGFSYAADLVRHIRSEFGDYFDICVAGYPKGHPDAGSFEADLKYLKEKVSAGADFIITQLFFEADTFFHFVKACSEIGITCPILPGIFPIQGYHSLRQLVKLSKLEVPQQIKDVIEPIKDNDAAIRNYGIEQAVSLCQELLTSGLVPGLHFYTLNREMATTEVLKRLGMWIEDPRRPLPWAVSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFARYLSGEPNQHGYKVTCLPWNDEPLAAETSLMKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETVGALLQVLKKYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDNCLWQVVEDTFELLKGPSPEREAEAV

Target ORF information:

RefSeq Version	XM_005617992.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris methylenetetrahydrofolate reductase (MTHFR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617992.3

ORF Insert Sequence:

ATGGACCAGC CACCACCTGG GGTTCTCCCA GCCACAGTCC GCTGCTCCCG CCTAGGACTG 
TGGACTCTGG AGGCCGGCAG GGTCGGGTTC TCAGTGCCAC CCTCCATCAG CAGGAGCCCG 
GCCATGGTGA AGGAGCCCAG AGGGGACGGG GACGGCGGTC CCACTCCCCG CTCGGAGGGC 
AGCAGCAGCG GCAGCGAGAG CTCCAAGGAT AGTTCGAGAT GTTCCACGCC GGGGCTGGAC 
CCTGAGCGCC ACGAGAGACT CAGGGAGAAG ATGAGGCGGA GGATGGAATC CGGTGACAGG 
TGGTTCTCCT TGGAGTTCTT TCCTCCTCGG ACTGCTGAGG GAGCGGTCAA CCTCATCTCG 
AGGTTTGATC GGATGGGGGC AGGTGGCCCC CTCTTCATAG ATGTGACCTG GCACCCGGCA 
GGGGACCCTG GCTCAGACAA GGAGACGTCC TCCATGATGA TTGCCAGCAC CGCCGTGAAC 
TACTGTGGCC TGGAGACCAT CCTGCACATG ACCTGCTGCT GTCAGAGCCG GGAGCAGATC 
ACAGGCTATC TGCACAAGGC CAAGCGGCTG GGCCTGAAGA ACATCTTGGC GCTGAGGGGA 
GACCCCGTGG GCGACCAGTG GGAAGTTGTG GAAGGAGGCT TCAGCTATGC CGCGGACTTG 
GTGAGGCACA TCCGAAGCGA GTTTGGGGAC TACTTTGACA TCTGTGTGGC AGGTTACCCC 
AAAGGCCACC CCGACGCAGG GAGCTTTGAG GCCGACCTGA AGTACTTGAA GGAGAAGGTA 
TCCGCAGGAG CCGACTTCAT CATCACCCAG CTTTTCTTTG AGGCTGACAC ATTCTTCCAC 
TTTGTTAAGG CTTGCAGCGA GATAGGCATA ACCTGCCCCA TCCTCCCCGG AATCTTTCCT 
ATTCAGGGCT ACCACTCCCT TCGGCAGCTC GTGAAACTGT CCAAGCTGGA GGTACCGCAG 
CAGATCAAAG ATGTGATTGA GCCAATCAAA GACAATGATG CTGCGATCCG TAACTATGGC 
ATCGAGCAGG CTGTGAGCCT GTGCCAGGAG CTTCTGACCA GTGGCTTGGT GCCAGGCCTC 
CACTTCTACA CCCTCAACCG CGAGATGGCC ACCACAGAGG TGCTGAAGCG CCTGGGCATG 
TGGATCGAGG ACCCCAGGCG TCCCCTACCC TGGGCTGTCA GTGCCCACCC CAAGCGCAGG 
GAGGAAGATG TACGCCCCAT TTTCTGGGCC TCCAGACCAA AGAGTTACAT CTACCGTACA 
CAAGAGTGGG ATGAGTTCCC CAATGGCCGC TGGGGCAACT CCTCCTCTCC AGCCTTCGGG 
GAGCTGAAGG ACTACTACCT CTTCTACCTG AAGAGCAAGT CCCCGAGGGA GGAGCTACTG 
AAGATGTGGG GGGAGGAGCT GACCAGTGAA GAAAGCGTCT TTGAAGTCTT TGCCCGCTAC 
CTCTCGGGAG AGCCCAACCA GCACGGCTAC AAGGTGACTT GCCTGCCCTG GAATGACGAG 
CCCTTGGCGG CCGAGACCAG CCTGATGAAG GAGGAGCTGC TGCGAGTGAA CCGGCAGGGC 
ATCCTCACCA TCAACTCCCA GCCCAACATC AACGGGAAAC CATCCTCTGA CCCCATCGTG 
GGCTGGGGCC CCAGCGGGGG CTATGTCTTC CAGAAGGCCT ACTTGGAGTT CTTCACTTCC 
CGCGAGACGG TGGGGGCGCT TCTGCAGGTG CTGAAGAAGT ATGAGCTCCG GGTTAATTAC 
CACATCGTGG ACGTGAAGGG TGAAAACATC ACCAATGCGC CTGAGCTGCA GCCCAATGCG 
GTCACTTGGG GCATCTTCCC GGGGCGGGAG ATCATCCAGC CTACGGTGGT AGACCCTGTC 
AGCTTCATGT TCTGGAAGGA TGAAGCCTTC GCCCTGTGGA TCGAGCAGTG GGGCAAGCTG 
TATGAGGAGG AGTCCCCGTC CCGCATGATC ATCCAGTACA TCCACGACAA CTACTTCCTG 
GTCAACCTGG TGGACAATGA GTTTCCGCTG GACAACTGCC TGTGGCAAGT GGTGGAAGAC 
ACGTTTGAAC TTCTCAAGGG GCCCTCACCA GAGCGAGAGG CAGAGGCCGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFR ( XM_005617992.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618049.1 Canis lupus familiaris methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X1, mRNA.

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CloneID	OCe03864
Clone ID Related Accession (Same CDS sequence)	XM_005617992.3 , XM_005617992.2
Accession Version	XM_005617992.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	methylenetetrahydrofolate reductase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726051.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAGC CACCACCTGG GGTTCTCCCA GCCACAGTCC GCTGCTCCCG CCTAGGACTG 
TGGACTCTGG AGGCCGGCAG GGTCGGGTTC TCAGTGCCAC CCTCCATCAG CAGGAGCCCG 
GCCATGGTGA AGGAGCCCAG AGGGGACGGG GACGGCGGTC CCACTCCCCG CTCGGAGGGC 
AGCAGCAGCG GCAGCGAGAG CTCCAAGGAT AGTTCGAGAT GTTCCACGCC GGGGCTGGAC 
CCTGAGCGCC ACGAGAGACT CAGGGAGAAG ATGAGGCGGA GGATGGAATC CGGTGACAGG 
TGGTTCTCCT TGGAGTTCTT TCCTCCTCGG ACTGCTGAGG GAGCGGTCAA CCTCATCTCG 
AGGTTTGATC GGATGGGGGC AGGTGGCCCC CTCTTCATAG ATGTGACCTG GCACCCGGCA 
GGGGACCCTG GCTCAGACAA GGAGACGTCC TCCATGATGA TTGCCAGCAC CGCCGTGAAC 
TACTGTGGCC TGGAGACCAT CCTGCACATG ACCTGCTGCT GTCAGAGCCG GGAGCAGATC 
ACAGGCTATC TGCACAAGGC CAAGCGGCTG GGCCTGAAGA ACATCTTGGC GCTGAGGGGA 
GACCCCGTGG GCGACCAGTG GGAAGTTGTG GAAGGAGGCT TCAGCTATGC CGCGGACTTG 
GTGAGGCACA TCCGAAGCGA GTTTGGGGAC TACTTTGACA TCTGTGTGGC AGGTTACCCC 
AAAGGCCACC CCGACGCAGG GAGCTTTGAG GCCGACCTGA AGTACTTGAA GGAGAAGGTA 
TCCGCAGGAG CCGACTTCAT CATCACCCAG CTTTTCTTTG AGGCTGACAC ATTCTTCCAC 
TTTGTTAAGG CTTGCAGCGA GATAGGCATA ACCTGCCCCA TCCTCCCCGG AATCTTTCCT 
ATTCAGGGCT ACCACTCCCT TCGGCAGCTC GTGAAACTGT CCAAGCTGGA GGTACCGCAG 
CAGATCAAAG ATGTGATTGA GCCAATCAAA GACAATGATG CTGCGATCCG TAACTATGGC 
ATCGAGCAGG CTGTGAGCCT GTGCCAGGAG CTTCTGACCA GTGGCTTGGT GCCAGGCCTC 
CACTTCTACA CCCTCAACCG CGAGATGGCC ACCACAGAGG TGCTGAAGCG CCTGGGCATG 
TGGATCGAGG ACCCCAGGCG TCCCCTACCC TGGGCTGTCA GTGCCCACCC CAAGCGCAGG 
GAGGAAGATG TACGCCCCAT TTTCTGGGCC TCCAGACCAA AGAGTTACAT CTACCGTACA 
CAAGAGTGGG ATGAGTTCCC CAATGGCCGC TGGGGCAACT CCTCCTCTCC AGCCTTCGGG 
GAGCTGAAGG ACTACTACCT CTTCTACCTG AAGAGCAAGT CCCCGAGGGA GGAGCTACTG 
AAGATGTGGG GGGAGGAGCT GACCAGTGAA GAAAGCGTCT TTGAAGTCTT TGCCCGCTAC 
CTCTCGGGAG AGCCCAACCA GCACGGCTAC AAGGTGACTT GCCTGCCCTG GAATGACGAG 
CCCTTGGCGG CCGAGACCAG CCTGATGAAG GAGGAGCTGC TGCGAGTGAA CCGGCAGGGC 
ATCCTCACCA TCAACTCCCA GCCCAACATC AACGGGAAAC CATCCTCTGA CCCCATCGTG 
GGCTGGGGCC CCAGCGGGGG CTATGTCTTC CAGAAGGCCT ACTTGGAGTT CTTCACTTCC 
CGCGAGACGG TGGGGGCGCT TCTGCAGGTG CTGAAGAAGT ATGAGCTCCG GGTTAATTAC 
CACATCGTGG ACGTGAAGGG TGAAAACATC ACCAATGCGC CTGAGCTGCA GCCCAATGCG 
GTCACTTGGG GCATCTTCCC GGGGCGGGAG ATCATCCAGC CTACGGTGGT AGACCCTGTC 
AGCTTCATGT TCTGGAAGGA TGAAGCCTTC GCCCTGTGGA TCGAGCAGTG GGGCAAGCTG 
TATGAGGAGG AGTCCCCGTC CCGCATGATC ATCCAGTACA TCCACGACAA CTACTTCCTG 
GTCAACCTGG TGGACAATGA GTTTCCGCTG GACAACTGCC TGTGGCAAGT GGTGGAAGAC 
ACGTTTGAAC TTCTCAAGGG GCCCTCACCA GAGCGAGAGG CAGAGGCCGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618049.1
CDS	168..2261
Translation	MDQPPPGVLPATVRCSRLGLWTLEAGRVGFSVPPSISRSPAMVKEPRGDGDGGPTPRSEGSSSGSESSKDSSRCSTPGLDPERHERLREKMRRRMESGDRWFSLEFFPPRTAEGAVNLISRFDRMGAGGPLFIDVTWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCCQSREQITGYLHKAKRLGLKNILALRGDPVGDQWEVVEGGFSYAADLVRHIRSEFGDYFDICVAGYPKGHPDAGSFEADLKYLKEKVSAGADFIITQLFFEADTFFHFVKACSEIGITCPILPGIFPIQGYHSLRQLVKLSKLEVPQQIKDVIEPIKDNDAAIRNYGIEQAVSLCQELLTSGLVPGLHFYTLNREMATTEVLKRLGMWIEDPRRPLPWAVSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFARYLSGEPNQHGYKVTCLPWNDEPLAAETSLMKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETVGALLQVLKKYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDNCLWQVVEDTFELLKGPSPEREAEAV

Target ORF information:

RefSeq Version	XM_005617992.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617992.2

ORF Insert Sequence:

ATGGACCAGC CACCACCTGG GGTTCTCCCA GCCACAGTCC GCTGCTCCCG CCTAGGACTG 
TGGACTCTGG AGGCCGGCAG GGTCGGGTTC TCAGTGCCAC CCTCCATCAG CAGGAGCCCG 
GCCATGGTGA AGGAGCCCAG AGGGGACGGG GACGGCGGTC CCACTCCCCG CTCGGAGGGC 
AGCAGCAGCG GCAGCGAGAG CTCCAAGGAT AGTTCGAGAT GTTCCACGCC GGGGCTGGAC 
CCTGAGCGCC ACGAGAGACT CAGGGAGAAG ATGAGGCGGA GGATGGAATC CGGTGACAGG 
TGGTTCTCCT TGGAGTTCTT TCCTCCTCGG ACTGCTGAGG GAGCGGTCAA CCTCATCTCG 
AGGTTTGATC GGATGGGGGC AGGTGGCCCC CTCTTCATAG ATGTGACCTG GCACCCGGCA 
GGGGACCCTG GCTCAGACAA GGAGACGTCC TCCATGATGA TTGCCAGCAC CGCCGTGAAC 
TACTGTGGCC TGGAGACCAT CCTGCACATG ACCTGCTGCT GTCAGAGCCG GGAGCAGATC 
ACAGGCTATC TGCACAAGGC CAAGCGGCTG GGCCTGAAGA ACATCTTGGC GCTGAGGGGA 
GACCCCGTGG GCGACCAGTG GGAAGTTGTG GAAGGAGGCT TCAGCTATGC CGCGGACTTG 
GTGAGGCACA TCCGAAGCGA GTTTGGGGAC TACTTTGACA TCTGTGTGGC AGGTTACCCC 
AAAGGCCACC CCGACGCAGG GAGCTTTGAG GCCGACCTGA AGTACTTGAA GGAGAAGGTA 
TCCGCAGGAG CCGACTTCAT CATCACCCAG CTTTTCTTTG AGGCTGACAC ATTCTTCCAC 
TTTGTTAAGG CTTGCAGCGA GATAGGCATA ACCTGCCCCA TCCTCCCCGG AATCTTTCCT 
ATTCAGGGCT ACCACTCCCT TCGGCAGCTC GTGAAACTGT CCAAGCTGGA GGTACCGCAG 
CAGATCAAAG ATGTGATTGA GCCAATCAAA GACAATGATG CTGCGATCCG TAACTATGGC 
ATCGAGCAGG CTGTGAGCCT GTGCCAGGAG CTTCTGACCA GTGGCTTGGT GCCAGGCCTC 
CACTTCTACA CCCTCAACCG CGAGATGGCC ACCACAGAGG TGCTGAAGCG CCTGGGCATG 
TGGATCGAGG ACCCCAGGCG TCCCCTACCC TGGGCTGTCA GTGCCCACCC CAAGCGCAGG 
GAGGAAGATG TACGCCCCAT TTTCTGGGCC TCCAGACCAA AGAGTTACAT CTACCGTACA 
CAAGAGTGGG ATGAGTTCCC CAATGGCCGC TGGGGCAACT CCTCCTCTCC AGCCTTCGGG 
GAGCTGAAGG ACTACTACCT CTTCTACCTG AAGAGCAAGT CCCCGAGGGA GGAGCTACTG 
AAGATGTGGG GGGAGGAGCT GACCAGTGAA GAAAGCGTCT TTGAAGTCTT TGCCCGCTAC 
CTCTCGGGAG AGCCCAACCA GCACGGCTAC AAGGTGACTT GCCTGCCCTG GAATGACGAG 
CCCTTGGCGG CCGAGACCAG CCTGATGAAG GAGGAGCTGC TGCGAGTGAA CCGGCAGGGC 
ATCCTCACCA TCAACTCCCA GCCCAACATC AACGGGAAAC CATCCTCTGA CCCCATCGTG 
GGCTGGGGCC CCAGCGGGGG CTATGTCTTC CAGAAGGCCT ACTTGGAGTT CTTCACTTCC 
CGCGAGACGG TGGGGGCGCT TCTGCAGGTG CTGAAGAAGT ATGAGCTCCG GGTTAATTAC 
CACATCGTGG ACGTGAAGGG TGAAAACATC ACCAATGCGC CTGAGCTGCA GCCCAATGCG 
GTCACTTGGG GCATCTTCCC GGGGCGGGAG ATCATCCAGC CTACGGTGGT AGACCCTGTC 
AGCTTCATGT TCTGGAAGGA TGAAGCCTTC GCCCTGTGGA TCGAGCAGTG GGGCAAGCTG 
TATGAGGAGG AGTCCCCGTC CCGCATGATC ATCCAGTACA TCCACGACAA CTACTTCCTG 
GTCAACCTGG TGGACAATGA GTTTCCGCTG GACAACTGCC TGTGGCAAGT GGTGGAAGAC 
ACGTTTGAAC TTCTCAAGGG GCCCTCACCA GAGCGAGAGG CAGAGGCCGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFR ( XM_014111461.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966936.1 Canis lupus familiaris methylenetetrahydrofolate reductase (MTHFR), transcript variant X3, mRNA.

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CloneID	OCe35535
Clone ID Related Accession (Same CDS sequence)	XM_014111461.2 , XM_014111460.1 , XM_014111461.1
Accession Version	XM_014111461.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	methylenetetrahydrofolate reductase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAAAT GCCCTGTTAT AGCTGGTCAG ACAGGCACGT GTGCGCACCT GTGGCTCTTG 
CGTCTCGTTC CGGAGCTGTG GCCGTGTTCT GGCGAGCCTC TGCTTGCCAC TCTGAGAATT 
CCCTGTACCT TTGATCATCA AAATATCTTA TCACCCAAGA TAACAAACTG CAAGCACTTT 
GGAAAGGCTG CAGTGCTGCT CTCTCACAAA AGGGTTGCAT TGTTGACATC AGGGGACCCT 
GGCTCAGACA AGGAGACGTC CTCCATGATG ATTGCCAGCA CCGCCGTGAA CTACTGTGGC 
CTGGAGACCA TCCTGCACAT GACCTGCTGC TGTCAGAGCC GGGAGCAGAT CACAGGCTAT 
CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGCTGAGGGG AGACCCCGTG 
GGCGACCAGT GGGAAGTTGT GGAAGGAGGC TTCAGCTATG CCGCGGACTT GGTGAGGCAC 
ATCCGAAGCG AGTTTGGGGA CTACTTTGAC ATCTGTGTGG CAGGTTACCC CAAAGGCCAC 
CCCGACGCAG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT ATCCGCAGGA 
GCCGACTTCA TCATCACCCA GCTTTTCTTT GAGGCTGACA CATTCTTCCA CTTTGTTAAG 
GCTTGCAGCG AGATAGGCAT AACCTGCCCC ATCCTCCCCG GAATCTTTCC TATTCAGGGC 
TACCACTCCC TTCGGCAGCT CGTGAAACTG TCCAAGCTGG AGGTACCGCA GCAGATCAAA 
GATGTGATTG AGCCAATCAA AGACAATGAT GCTGCGATCC GTAACTATGG CATCGAGCAG 
GCTGTGAGCC TGTGCCAGGA GCTTCTGACC AGTGGCTTGG TGCCAGGCCT CCACTTCTAC 
ACCCTCAACC GCGAGATGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGATCGAG 
GACCCCAGGC GTCCCCTACC CTGGGCTGTC AGTGCCCACC CCAAGCGCAG GGAGGAAGAT 
GTACGCCCCA TTTTCTGGGC CTCCAGACCA AAGAGTTACA TCTACCGTAC ACAAGAGTGG 
GATGAGTTCC CCAATGGCCG CTGGGGCAAC TCCTCCTCTC CAGCCTTCGG GGAGCTGAAG 
GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAGGG AGGAGCTACT GAAGATGTGG 
GGGGAGGAGC TGACCAGTGA AGAAAGCGTC TTTGAAGTCT TTGCCCGCTA CCTCTCGGGA 
GAGCCCAACC AGCACGGCTA CAAGGTGACT TGCCTGCCCT GGAATGACGA GCCCTTGGCG 
GCCGAGACCA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CATCCTCACC 
ATCAACTCCC AGCCCAACAT CAACGGGAAA CCATCCTCTG ACCCCATCGT GGGCTGGGGC 
CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTGGAGT TCTTCACTTC CCGCGAGACG 
GTGGGGGCGC TTCTGCAGGT GCTGAAGAAG TATGAGCTCC GGGTTAATTA CCACATCGTG 
GACGTGAAGG GTGAAAACAT CACCAATGCG CCTGAGCTGC AGCCCAATGC GGTCACTTGG 
GGCATCTTCC CGGGGCGGGA GATCATCCAG CCTACGGTGG TAGACCCTGT CAGCTTCATG 
TTCTGGAAGG ATGAAGCCTT CGCCCTGTGG ATCGAGCAGT GGGGCAAGCT GTATGAGGAG 
GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG 
GTGGACAATG AGTTTCCGCT GGACAACTGC CTGTGGCAAG TGGTGGAAGA CACGTTTGAA 
CTTCTCAAGG GGCCCTCACC AGAGCGAGAG GCAGAGGCCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966936.1
CDS	161..2065
Translation	MFKCPVIAGQTGTCAHLWLLRLVPELWPCSGEPLLATLRIPCTFDHQNILSPKITNCKHFGKAAVLLSHKRVALLTSGDPGSDKETSSMMIASTAVNYCGLETILHMTCCCQSREQITGYLHKAKRLGLKNILALRGDPVGDQWEVVEGGFSYAADLVRHIRSEFGDYFDICVAGYPKGHPDAGSFEADLKYLKEKVSAGADFIITQLFFEADTFFHFVKACSEIGITCPILPGIFPIQGYHSLRQLVKLSKLEVPQQIKDVIEPIKDNDAAIRNYGIEQAVSLCQELLTSGLVPGLHFYTLNREMATTEVLKRLGMWIEDPRRPLPWAVSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFARYLSGEPNQHGYKVTCLPWNDEPLAAETSLMKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETVGALLQVLKKYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDNCLWQVVEDTFELLKGPSPEREAEAV

Target ORF information:

RefSeq Version	XM_014111461.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris methylenetetrahydrofolate reductase (MTHFR), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111461.2

ORF Insert Sequence:

ATGTTTAAAT GCCCTGTTAT AGCTGGTCAG ACAGGCACGT GTGCGCACCT GTGGCTCTTG 
CGTCTCGTTC CGGAGCTGTG GCCGTGTTCT GGCGAGCCTC TGCTTGCCAC TCTGAGAATT 
CCCTGTACCT TTGATCATCA AAATATCTTA TCACCCAAGA TAACAAACTG CAAGCACTTT 
GGAAAGGCTG CAGTGCTGCT CTCTCACAAA AGGGTTGCAT TGTTGACATC AGGGGACCCT 
GGCTCAGACA AGGAGACGTC CTCCATGATG ATTGCCAGCA CCGCCGTGAA CTACTGTGGC 
CTGGAGACCA TCCTGCACAT GACCTGCTGC TGTCAGAGCC GGGAGCAGAT CACAGGCTAT 
CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGCTGAGGGG AGACCCCGTG 
GGCGACCAGT GGGAAGTTGT GGAAGGAGGC TTCAGCTATG CCGCGGACTT GGTGAGGCAC 
ATCCGAAGCG AGTTTGGGGA CTACTTTGAC ATCTGTGTGG CAGGTTACCC CAAAGGCCAC 
CCCGACGCAG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT ATCCGCAGGA 
GCCGACTTCA TCATCACCCA GCTTTTCTTT GAGGCTGACA CATTCTTCCA CTTTGTTAAG 
GCTTGCAGCG AGATAGGCAT AACCTGCCCC ATCCTCCCCG GAATCTTTCC TATTCAGGGC 
TACCACTCCC TTCGGCAGCT CGTGAAACTG TCCAAGCTGG AGGTACCGCA GCAGATCAAA 
GATGTGATTG AGCCAATCAA AGACAATGAT GCTGCGATCC GTAACTATGG CATCGAGCAG 
GCTGTGAGCC TGTGCCAGGA GCTTCTGACC AGTGGCTTGG TGCCAGGCCT CCACTTCTAC 
ACCCTCAACC GCGAGATGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGATCGAG 
GACCCCAGGC GTCCCCTACC CTGGGCTGTC AGTGCCCACC CCAAGCGCAG GGAGGAAGAT 
GTACGCCCCA TTTTCTGGGC CTCCAGACCA AAGAGTTACA TCTACCGTAC ACAAGAGTGG 
GATGAGTTCC CCAATGGCCG CTGGGGCAAC TCCTCCTCTC CAGCCTTCGG GGAGCTGAAG 
GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAGGG AGGAGCTACT GAAGATGTGG 
GGGGAGGAGC TGACCAGTGA AGAAAGCGTC TTTGAAGTCT TTGCCCGCTA CCTCTCGGGA 
GAGCCCAACC AGCACGGCTA CAAGGTGACT TGCCTGCCCT GGAATGACGA GCCCTTGGCG 
GCCGAGACCA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CATCCTCACC 
ATCAACTCCC AGCCCAACAT CAACGGGAAA CCATCCTCTG ACCCCATCGT GGGCTGGGGC 
CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTGGAGT TCTTCACTTC CCGCGAGACG 
GTGGGGGCGC TTCTGCAGGT GCTGAAGAAG TATGAGCTCC GGGTTAATTA CCACATCGTG 
GACGTGAAGG GTGAAAACAT CACCAATGCG CCTGAGCTGC AGCCCAATGC GGTCACTTGG 
GGCATCTTCC CGGGGCGGGA GATCATCCAG CCTACGGTGG TAGACCCTGT CAGCTTCATG 
TTCTGGAAGG ATGAAGCCTT CGCCCTGTGG ATCGAGCAGT GGGGCAAGCT GTATGAGGAG 
GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG 
GTGGACAATG AGTTTCCGCT GGACAACTGC CTGTGGCAAG TGGTGGAAGA CACGTTTGAA 
CTTCTCAAGG GGCCCTCACC AGAGCGAGAG GCAGAGGCCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFR ( XM_014111460.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966935.1 Canis lupus familiaris methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe35535
Clone ID Related Accession (Same CDS sequence)	XM_014111461.2 , XM_014111460.1 , XM_014111461.1
Accession Version	XM_014111460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	methylenetetrahydrofolate reductase isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726051.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAAAT GCCCTGTTAT AGCTGGTCAG ACAGGCACGT GTGCGCACCT GTGGCTCTTG 
CGTCTCGTTC CGGAGCTGTG GCCGTGTTCT GGCGAGCCTC TGCTTGCCAC TCTGAGAATT 
CCCTGTACCT TTGATCATCA AAATATCTTA TCACCCAAGA TAACAAACTG CAAGCACTTT 
GGAAAGGCTG CAGTGCTGCT CTCTCACAAA AGGGTTGCAT TGTTGACATC AGGGGACCCT 
GGCTCAGACA AGGAGACGTC CTCCATGATG ATTGCCAGCA CCGCCGTGAA CTACTGTGGC 
CTGGAGACCA TCCTGCACAT GACCTGCTGC TGTCAGAGCC GGGAGCAGAT CACAGGCTAT 
CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGCTGAGGGG AGACCCCGTG 
GGCGACCAGT GGGAAGTTGT GGAAGGAGGC TTCAGCTATG CCGCGGACTT GGTGAGGCAC 
ATCCGAAGCG AGTTTGGGGA CTACTTTGAC ATCTGTGTGG CAGGTTACCC CAAAGGCCAC 
CCCGACGCAG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT ATCCGCAGGA 
GCCGACTTCA TCATCACCCA GCTTTTCTTT GAGGCTGACA CATTCTTCCA CTTTGTTAAG 
GCTTGCAGCG AGATAGGCAT AACCTGCCCC ATCCTCCCCG GAATCTTTCC TATTCAGGGC 
TACCACTCCC TTCGGCAGCT CGTGAAACTG TCCAAGCTGG AGGTACCGCA GCAGATCAAA 
GATGTGATTG AGCCAATCAA AGACAATGAT GCTGCGATCC GTAACTATGG CATCGAGCAG 
GCTGTGAGCC TGTGCCAGGA GCTTCTGACC AGTGGCTTGG TGCCAGGCCT CCACTTCTAC 
ACCCTCAACC GCGAGATGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGATCGAG 
GACCCCAGGC GTCCCCTACC CTGGGCTGTC AGTGCCCACC CCAAGCGCAG GGAGGAAGAT 
GTACGCCCCA TTTTCTGGGC CTCCAGACCA AAGAGTTACA TCTACCGTAC ACAAGAGTGG 
GATGAGTTCC CCAATGGCCG CTGGGGCAAC TCCTCCTCTC CAGCCTTCGG GGAGCTGAAG 
GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAGGG AGGAGCTACT GAAGATGTGG 
GGGGAGGAGC TGACCAGTGA AGAAAGCGTC TTTGAAGTCT TTGCCCGCTA CCTCTCGGGA 
GAGCCCAACC AGCACGGCTA CAAGGTGACT TGCCTGCCCT GGAATGACGA GCCCTTGGCG 
GCCGAGACCA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CATCCTCACC 
ATCAACTCCC AGCCCAACAT CAACGGGAAA CCATCCTCTG ACCCCATCGT GGGCTGGGGC 
CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTGGAGT TCTTCACTTC CCGCGAGACG 
GTGGGGGCGC TTCTGCAGGT GCTGAAGAAG TATGAGCTCC GGGTTAATTA CCACATCGTG 
GACGTGAAGG GTGAAAACAT CACCAATGCG CCTGAGCTGC AGCCCAATGC GGTCACTTGG 
GGCATCTTCC CGGGGCGGGA GATCATCCAG CCTACGGTGG TAGACCCTGT CAGCTTCATG 
TTCTGGAAGG ATGAAGCCTT CGCCCTGTGG ATCGAGCAGT GGGGCAAGCT GTATGAGGAG 
GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG 
GTGGACAATG AGTTTCCGCT GGACAACTGC CTGTGGCAAG TGGTGGAAGA CACGTTTGAA 
CTTCTCAAGG GGCCCTCACC AGAGCGAGAG GCAGAGGCCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966935.1
CDS	107..2011
Translation	MFKCPVIAGQTGTCAHLWLLRLVPELWPCSGEPLLATLRIPCTFDHQNILSPKITNCKHFGKAAVLLSHKRVALLTSGDPGSDKETSSMMIASTAVNYCGLETILHMTCCCQSREQITGYLHKAKRLGLKNILALRGDPVGDQWEVVEGGFSYAADLVRHIRSEFGDYFDICVAGYPKGHPDAGSFEADLKYLKEKVSAGADFIITQLFFEADTFFHFVKACSEIGITCPILPGIFPIQGYHSLRQLVKLSKLEVPQQIKDVIEPIKDNDAAIRNYGIEQAVSLCQELLTSGLVPGLHFYTLNREMATTEVLKRLGMWIEDPRRPLPWAVSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFARYLSGEPNQHGYKVTCLPWNDEPLAAETSLMKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETVGALLQVLKKYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDNCLWQVVEDTFELLKGPSPEREAEAV

Target ORF information:

RefSeq Version	XM_014111460.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111460.1

ORF Insert Sequence:

ATGTTTAAAT GCCCTGTTAT AGCTGGTCAG ACAGGCACGT GTGCGCACCT GTGGCTCTTG 
CGTCTCGTTC CGGAGCTGTG GCCGTGTTCT GGCGAGCCTC TGCTTGCCAC TCTGAGAATT 
CCCTGTACCT TTGATCATCA AAATATCTTA TCACCCAAGA TAACAAACTG CAAGCACTTT 
GGAAAGGCTG CAGTGCTGCT CTCTCACAAA AGGGTTGCAT TGTTGACATC AGGGGACCCT 
GGCTCAGACA AGGAGACGTC CTCCATGATG ATTGCCAGCA CCGCCGTGAA CTACTGTGGC 
CTGGAGACCA TCCTGCACAT GACCTGCTGC TGTCAGAGCC GGGAGCAGAT CACAGGCTAT 
CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGCTGAGGGG AGACCCCGTG 
GGCGACCAGT GGGAAGTTGT GGAAGGAGGC TTCAGCTATG CCGCGGACTT GGTGAGGCAC 
ATCCGAAGCG AGTTTGGGGA CTACTTTGAC ATCTGTGTGG CAGGTTACCC CAAAGGCCAC 
CCCGACGCAG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT ATCCGCAGGA 
GCCGACTTCA TCATCACCCA GCTTTTCTTT GAGGCTGACA CATTCTTCCA CTTTGTTAAG 
GCTTGCAGCG AGATAGGCAT AACCTGCCCC ATCCTCCCCG GAATCTTTCC TATTCAGGGC 
TACCACTCCC TTCGGCAGCT CGTGAAACTG TCCAAGCTGG AGGTACCGCA GCAGATCAAA 
GATGTGATTG AGCCAATCAA AGACAATGAT GCTGCGATCC GTAACTATGG CATCGAGCAG 
GCTGTGAGCC TGTGCCAGGA GCTTCTGACC AGTGGCTTGG TGCCAGGCCT CCACTTCTAC 
ACCCTCAACC GCGAGATGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGATCGAG 
GACCCCAGGC GTCCCCTACC CTGGGCTGTC AGTGCCCACC CCAAGCGCAG GGAGGAAGAT 
GTACGCCCCA TTTTCTGGGC CTCCAGACCA AAGAGTTACA TCTACCGTAC ACAAGAGTGG 
GATGAGTTCC CCAATGGCCG CTGGGGCAAC TCCTCCTCTC CAGCCTTCGG GGAGCTGAAG 
GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAGGG AGGAGCTACT GAAGATGTGG 
GGGGAGGAGC TGACCAGTGA AGAAAGCGTC TTTGAAGTCT TTGCCCGCTA CCTCTCGGGA 
GAGCCCAACC AGCACGGCTA CAAGGTGACT TGCCTGCCCT GGAATGACGA GCCCTTGGCG 
GCCGAGACCA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CATCCTCACC 
ATCAACTCCC AGCCCAACAT CAACGGGAAA CCATCCTCTG ACCCCATCGT GGGCTGGGGC 
CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTGGAGT TCTTCACTTC CCGCGAGACG 
GTGGGGGCGC TTCTGCAGGT GCTGAAGAAG TATGAGCTCC GGGTTAATTA CCACATCGTG 
GACGTGAAGG GTGAAAACAT CACCAATGCG CCTGAGCTGC AGCCCAATGC GGTCACTTGG 
GGCATCTTCC CGGGGCGGGA GATCATCCAG CCTACGGTGG TAGACCCTGT CAGCTTCATG 
TTCTGGAAGG ATGAAGCCTT CGCCCTGTGG ATCGAGCAGT GGGGCAAGCT GTATGAGGAG 
GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG 
GTGGACAATG AGTTTCCGCT GGACAACTGC CTGTGGCAAG TGGTGGAAGA CACGTTTGAA 
CTTCTCAAGG GGCCCTCACC AGAGCGAGAG GCAGAGGCCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFR ( XM_014111461.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966936.1 Canis lupus familiaris methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe35535
Clone ID Related Accession (Same CDS sequence)	XM_014111461.2 , XM_014111460.1 , XM_014111461.1
Accession Version	XM_014111461.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	methylenetetrahydrofolate reductase isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726051.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAAAT GCCCTGTTAT AGCTGGTCAG ACAGGCACGT GTGCGCACCT GTGGCTCTTG 
CGTCTCGTTC CGGAGCTGTG GCCGTGTTCT GGCGAGCCTC TGCTTGCCAC TCTGAGAATT 
CCCTGTACCT TTGATCATCA AAATATCTTA TCACCCAAGA TAACAAACTG CAAGCACTTT 
GGAAAGGCTG CAGTGCTGCT CTCTCACAAA AGGGTTGCAT TGTTGACATC AGGGGACCCT 
GGCTCAGACA AGGAGACGTC CTCCATGATG ATTGCCAGCA CCGCCGTGAA CTACTGTGGC 
CTGGAGACCA TCCTGCACAT GACCTGCTGC TGTCAGAGCC GGGAGCAGAT CACAGGCTAT 
CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGCTGAGGGG AGACCCCGTG 
GGCGACCAGT GGGAAGTTGT GGAAGGAGGC TTCAGCTATG CCGCGGACTT GGTGAGGCAC 
ATCCGAAGCG AGTTTGGGGA CTACTTTGAC ATCTGTGTGG CAGGTTACCC CAAAGGCCAC 
CCCGACGCAG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT ATCCGCAGGA 
GCCGACTTCA TCATCACCCA GCTTTTCTTT GAGGCTGACA CATTCTTCCA CTTTGTTAAG 
GCTTGCAGCG AGATAGGCAT AACCTGCCCC ATCCTCCCCG GAATCTTTCC TATTCAGGGC 
TACCACTCCC TTCGGCAGCT CGTGAAACTG TCCAAGCTGG AGGTACCGCA GCAGATCAAA 
GATGTGATTG AGCCAATCAA AGACAATGAT GCTGCGATCC GTAACTATGG CATCGAGCAG 
GCTGTGAGCC TGTGCCAGGA GCTTCTGACC AGTGGCTTGG TGCCAGGCCT CCACTTCTAC 
ACCCTCAACC GCGAGATGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGATCGAG 
GACCCCAGGC GTCCCCTACC CTGGGCTGTC AGTGCCCACC CCAAGCGCAG GGAGGAAGAT 
GTACGCCCCA TTTTCTGGGC CTCCAGACCA AAGAGTTACA TCTACCGTAC ACAAGAGTGG 
GATGAGTTCC CCAATGGCCG CTGGGGCAAC TCCTCCTCTC CAGCCTTCGG GGAGCTGAAG 
GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAGGG AGGAGCTACT GAAGATGTGG 
GGGGAGGAGC TGACCAGTGA AGAAAGCGTC TTTGAAGTCT TTGCCCGCTA CCTCTCGGGA 
GAGCCCAACC AGCACGGCTA CAAGGTGACT TGCCTGCCCT GGAATGACGA GCCCTTGGCG 
GCCGAGACCA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CATCCTCACC 
ATCAACTCCC AGCCCAACAT CAACGGGAAA CCATCCTCTG ACCCCATCGT GGGCTGGGGC 
CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTGGAGT TCTTCACTTC CCGCGAGACG 
GTGGGGGCGC TTCTGCAGGT GCTGAAGAAG TATGAGCTCC GGGTTAATTA CCACATCGTG 
GACGTGAAGG GTGAAAACAT CACCAATGCG CCTGAGCTGC AGCCCAATGC GGTCACTTGG 
GGCATCTTCC CGGGGCGGGA GATCATCCAG CCTACGGTGG TAGACCCTGT CAGCTTCATG 
TTCTGGAAGG ATGAAGCCTT CGCCCTGTGG ATCGAGCAGT GGGGCAAGCT GTATGAGGAG 
GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG 
GTGGACAATG AGTTTCCGCT GGACAACTGC CTGTGGCAAG TGGTGGAAGA CACGTTTGAA 
CTTCTCAAGG GGCCCTCACC AGAGCGAGAG GCAGAGGCCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966936.1
CDS	111..2015
Translation	MFKCPVIAGQTGTCAHLWLLRLVPELWPCSGEPLLATLRIPCTFDHQNILSPKITNCKHFGKAAVLLSHKRVALLTSGDPGSDKETSSMMIASTAVNYCGLETILHMTCCCQSREQITGYLHKAKRLGLKNILALRGDPVGDQWEVVEGGFSYAADLVRHIRSEFGDYFDICVAGYPKGHPDAGSFEADLKYLKEKVSAGADFIITQLFFEADTFFHFVKACSEIGITCPILPGIFPIQGYHSLRQLVKLSKLEVPQQIKDVIEPIKDNDAAIRNYGIEQAVSLCQELLTSGLVPGLHFYTLNREMATTEVLKRLGMWIEDPRRPLPWAVSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFARYLSGEPNQHGYKVTCLPWNDEPLAAETSLMKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETVGALLQVLKKYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDNCLWQVVEDTFELLKGPSPEREAEAV

Target ORF information:

RefSeq Version	XM_014111461.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111461.1

ORF Insert Sequence:

ATGTTTAAAT GCCCTGTTAT AGCTGGTCAG ACAGGCACGT GTGCGCACCT GTGGCTCTTG 
CGTCTCGTTC CGGAGCTGTG GCCGTGTTCT GGCGAGCCTC TGCTTGCCAC TCTGAGAATT 
CCCTGTACCT TTGATCATCA AAATATCTTA TCACCCAAGA TAACAAACTG CAAGCACTTT 
GGAAAGGCTG CAGTGCTGCT CTCTCACAAA AGGGTTGCAT TGTTGACATC AGGGGACCCT 
GGCTCAGACA AGGAGACGTC CTCCATGATG ATTGCCAGCA CCGCCGTGAA CTACTGTGGC 
CTGGAGACCA TCCTGCACAT GACCTGCTGC TGTCAGAGCC GGGAGCAGAT CACAGGCTAT 
CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGCTGAGGGG AGACCCCGTG 
GGCGACCAGT GGGAAGTTGT GGAAGGAGGC TTCAGCTATG CCGCGGACTT GGTGAGGCAC 
ATCCGAAGCG AGTTTGGGGA CTACTTTGAC ATCTGTGTGG CAGGTTACCC CAAAGGCCAC 
CCCGACGCAG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT ATCCGCAGGA 
GCCGACTTCA TCATCACCCA GCTTTTCTTT GAGGCTGACA CATTCTTCCA CTTTGTTAAG 
GCTTGCAGCG AGATAGGCAT AACCTGCCCC ATCCTCCCCG GAATCTTTCC TATTCAGGGC 
TACCACTCCC TTCGGCAGCT CGTGAAACTG TCCAAGCTGG AGGTACCGCA GCAGATCAAA 
GATGTGATTG AGCCAATCAA AGACAATGAT GCTGCGATCC GTAACTATGG CATCGAGCAG 
GCTGTGAGCC TGTGCCAGGA GCTTCTGACC AGTGGCTTGG TGCCAGGCCT CCACTTCTAC 
ACCCTCAACC GCGAGATGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGATCGAG 
GACCCCAGGC GTCCCCTACC CTGGGCTGTC AGTGCCCACC CCAAGCGCAG GGAGGAAGAT 
GTACGCCCCA TTTTCTGGGC CTCCAGACCA AAGAGTTACA TCTACCGTAC ACAAGAGTGG 
GATGAGTTCC CCAATGGCCG CTGGGGCAAC TCCTCCTCTC CAGCCTTCGG GGAGCTGAAG 
GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAGGG AGGAGCTACT GAAGATGTGG 
GGGGAGGAGC TGACCAGTGA AGAAAGCGTC TTTGAAGTCT TTGCCCGCTA CCTCTCGGGA 
GAGCCCAACC AGCACGGCTA CAAGGTGACT TGCCTGCCCT GGAATGACGA GCCCTTGGCG 
GCCGAGACCA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CATCCTCACC 
ATCAACTCCC AGCCCAACAT CAACGGGAAA CCATCCTCTG ACCCCATCGT GGGCTGGGGC 
CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTGGAGT TCTTCACTTC CCGCGAGACG 
GTGGGGGCGC TTCTGCAGGT GCTGAAGAAG TATGAGCTCC GGGTTAATTA CCACATCGTG 
GACGTGAAGG GTGAAAACAT CACCAATGCG CCTGAGCTGC AGCCCAATGC GGTCACTTGG 
GGCATCTTCC CGGGGCGGGA GATCATCCAG CCTACGGTGG TAGACCCTGT CAGCTTCATG 
TTCTGGAAGG ATGAAGCCTT CGCCCTGTGG ATCGAGCAGT GGGGCAAGCT GTATGAGGAG 
GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG 
GTGGACAATG AGTTTCCGCT GGACAACTGC CTGTGGCAAG TGGTGGAAGA CACGTTTGAA 
CTTCTCAAGG GGCCCTCACC AGAGCGAGAG GCAGAGGCCG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFR ( XM_014111464.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966939.1 Canis lupus familiaris methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe35536
Clone ID Related Accession (Same CDS sequence)	XM_014111464.1 , XM_014111464.2
Accession Version	XM_014111464.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	methylenetetrahydrofolate reductase isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726051.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCAG GTGGCCCCCT CTTCATAGAT GTGACCTGGC ACCCGGCAGG GGACCCTGGC 
TCAGACAAGG AGACGTCCTC CATGATGATT GCCAGCACCG CCGTGAACTA CTGTGGCCTG 
GAGACCATCC TGCACATGAC CTGCTGCTGT CAGAGCCGGG AGCAGATCAC AGGCTATCTG 
CACAAGGCCA AGCGGCTGGG CCTGAAGAAC ATCTTGGCGC TGAGGGGAGA CCCCGTGGGC 
GACCAGTGGG AAGTTGTGGA AGGAGGCTTC AGCTATGCCG CGGACTTGGT GAGGCACATC 
CGAAGCGAGT TTGGGGACTA CTTTGACATC TGTGTGGCAG GTTACCCCAA AGGCCACCCC 
GACGCAGGGA GCTTTGAGGC CGACCTGAAG TACTTGAAGG AGAAGGTATC CGCAGGAGCC 
GACTTCATCA TCACCCAGCT TTTCTTTGAG GCTGACACAT TCTTCCACTT TGTTAAGGCT 
TGCAGCGAGA TAGGCATAAC CTGCCCCATC CTCCCCGGAA TCTTTCCTAT TCAGGGCTAC 
CACTCCCTTC GGCAGCTCGT GAAACTGTCC AAGCTGGAGG TACCGCAGCA GATCAAAGAT 
GTGATTGAGC CAATCAAAGA CAATGATGCT GCGATCCGTA ACTATGGCAT CGAGCAGGCT 
GTGAGCCTGT GCCAGGAGCT TCTGACCAGT GGCTTGGTGC CAGGCCTCCA CTTCTACACC 
CTCAACCGCG AGATGGCCAC CACAGAGGTG CTGAAGCGCC TGGGCATGTG GATCGAGGAC 
CCCAGGCGTC CCCTACCCTG GGCTGTCAGT GCCCACCCCA AGCGCAGGGA GGAAGATGTA 
CGCCCCATTT TCTGGGCCTC CAGACCAAAG AGTTACATCT ACCGTACACA AGAGTGGGAT 
GAGTTCCCCA ATGGCCGCTG GGGCAACTCC TCCTCTCCAG CCTTCGGGGA GCTGAAGGAC 
TACTACCTCT TCTACCTGAA GAGCAAGTCC CCGAGGGAGG AGCTACTGAA GATGTGGGGG 
GAGGAGCTGA CCAGTGAAGA AAGCGTCTTT GAAGTCTTTG CCCGCTACCT CTCGGGAGAG 
CCCAACCAGC ACGGCTACAA GGTGACTTGC CTGCCCTGGA ATGACGAGCC CTTGGCGGCC 
GAGACCAGCC TGATGAAGGA GGAGCTGCTG CGAGTGAACC GGCAGGGCAT CCTCACCATC 
AACTCCCAGC CCAACATCAA CGGGAAACCA TCCTCTGACC CCATCGTGGG CTGGGGCCCC 
AGCGGGGGCT ATGTCTTCCA GAAGGCCTAC TTGGAGTTCT TCACTTCCCG CGAGACGGTG 
GGGGCGCTTC TGCAGGTGCT GAAGAAGTAT GAGCTCCGGG TTAATTACCA CATCGTGGAC 
GTGAAGGGTG AAAACATCAC CAATGCGCCT GAGCTGCAGC CCAATGCGGT CACTTGGGGC 
ATCTTCCCGG GGCGGGAGAT CATCCAGCCT ACGGTGGTAG ACCCTGTCAG CTTCATGTTC 
TGGAAGGATG AAGCCTTCGC CCTGTGGATC GAGCAGTGGG GCAAGCTGTA TGAGGAGGAG 
TCCCCGTCCC GCATGATCAT CCAGTACATC CACGACAACT ACTTCCTGGT CAACCTGGTG 
GACAATGAGT TTCCGCTGGA CAACTGCCTG TGGCAAGTGG TGGAAGACAC GTTTGAACTT 
CTCAAGGGGC CCTCACCAGA GCGAGAGGCA GAGGCCGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966939.1
CDS	85..1806
Translation	MGAGGPLFIDVTWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCCQSREQITGYLHKAKRLGLKNILALRGDPVGDQWEVVEGGFSYAADLVRHIRSEFGDYFDICVAGYPKGHPDAGSFEADLKYLKEKVSAGADFIITQLFFEADTFFHFVKACSEIGITCPILPGIFPIQGYHSLRQLVKLSKLEVPQQIKDVIEPIKDNDAAIRNYGIEQAVSLCQELLTSGLVPGLHFYTLNREMATTEVLKRLGMWIEDPRRPLPWAVSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFARYLSGEPNQHGYKVTCLPWNDEPLAAETSLMKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETVGALLQVLKKYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDNCLWQVVEDTFELLKGPSPEREAEAV

Target ORF information:

RefSeq Version	XM_014111464.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111464.1

ORF Insert Sequence:

ATGGGGGCAG GTGGCCCCCT CTTCATAGAT GTGACCTGGC ACCCGGCAGG GGACCCTGGC 
TCAGACAAGG AGACGTCCTC CATGATGATT GCCAGCACCG CCGTGAACTA CTGTGGCCTG 
GAGACCATCC TGCACATGAC CTGCTGCTGT CAGAGCCGGG AGCAGATCAC AGGCTATCTG 
CACAAGGCCA AGCGGCTGGG CCTGAAGAAC ATCTTGGCGC TGAGGGGAGA CCCCGTGGGC 
GACCAGTGGG AAGTTGTGGA AGGAGGCTTC AGCTATGCCG CGGACTTGGT GAGGCACATC 
CGAAGCGAGT TTGGGGACTA CTTTGACATC TGTGTGGCAG GTTACCCCAA AGGCCACCCC 
GACGCAGGGA GCTTTGAGGC CGACCTGAAG TACTTGAAGG AGAAGGTATC CGCAGGAGCC 
GACTTCATCA TCACCCAGCT TTTCTTTGAG GCTGACACAT TCTTCCACTT TGTTAAGGCT 
TGCAGCGAGA TAGGCATAAC CTGCCCCATC CTCCCCGGAA TCTTTCCTAT TCAGGGCTAC 
CACTCCCTTC GGCAGCTCGT GAAACTGTCC AAGCTGGAGG TACCGCAGCA GATCAAAGAT 
GTGATTGAGC CAATCAAAGA CAATGATGCT GCGATCCGTA ACTATGGCAT CGAGCAGGCT 
GTGAGCCTGT GCCAGGAGCT TCTGACCAGT GGCTTGGTGC CAGGCCTCCA CTTCTACACC 
CTCAACCGCG AGATGGCCAC CACAGAGGTG CTGAAGCGCC TGGGCATGTG GATCGAGGAC 
CCCAGGCGTC CCCTACCCTG GGCTGTCAGT GCCCACCCCA AGCGCAGGGA GGAAGATGTA 
CGCCCCATTT TCTGGGCCTC CAGACCAAAG AGTTACATCT ACCGTACACA AGAGTGGGAT 
GAGTTCCCCA ATGGCCGCTG GGGCAACTCC TCCTCTCCAG CCTTCGGGGA GCTGAAGGAC 
TACTACCTCT TCTACCTGAA GAGCAAGTCC CCGAGGGAGG AGCTACTGAA GATGTGGGGG 
GAGGAGCTGA CCAGTGAAGA AAGCGTCTTT GAAGTCTTTG CCCGCTACCT CTCGGGAGAG 
CCCAACCAGC ACGGCTACAA GGTGACTTGC CTGCCCTGGA ATGACGAGCC CTTGGCGGCC 
GAGACCAGCC TGATGAAGGA GGAGCTGCTG CGAGTGAACC GGCAGGGCAT CCTCACCATC 
AACTCCCAGC CCAACATCAA CGGGAAACCA TCCTCTGACC CCATCGTGGG CTGGGGCCCC 
AGCGGGGGCT ATGTCTTCCA GAAGGCCTAC TTGGAGTTCT TCACTTCCCG CGAGACGGTG 
GGGGCGCTTC TGCAGGTGCT GAAGAAGTAT GAGCTCCGGG TTAATTACCA CATCGTGGAC 
GTGAAGGGTG AAAACATCAC CAATGCGCCT GAGCTGCAGC CCAATGCGGT CACTTGGGGC 
ATCTTCCCGG GGCGGGAGAT CATCCAGCCT ACGGTGGTAG ACCCTGTCAG CTTCATGTTC 
TGGAAGGATG AAGCCTTCGC CCTGTGGATC GAGCAGTGGG GCAAGCTGTA TGAGGAGGAG 
TCCCCGTCCC GCATGATCAT CCAGTACATC CACGACAACT ACTTCCTGGT CAACCTGGTG 
GACAATGAGT TTCCGCTGGA CAACTGCCTG TGGCAAGTGG TGGAAGACAC GTTTGAACTT 
CTCAAGGGGC CCTCACCAGA GCGAGAGGCA GAGGCCGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFR ( XM_014111464.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966939.1 Canis lupus familiaris methylenetetrahydrofolate reductase (MTHFR), transcript variant X4, mRNA.

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CloneID	OCe35536
Clone ID Related Accession (Same CDS sequence)	XM_014111464.1 , XM_014111464.2
Accession Version	XM_014111464.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	methylenetetrahydrofolate reductase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111464.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCAG GTGGCCCCCT CTTCATAGAT GTGACCTGGC ACCCGGCAGG GGACCCTGGC 
TCAGACAAGG AGACGTCCTC CATGATGATT GCCAGCACCG CCGTGAACTA CTGTGGCCTG 
GAGACCATCC TGCACATGAC CTGCTGCTGT CAGAGCCGGG AGCAGATCAC AGGCTATCTG 
CACAAGGCCA AGCGGCTGGG CCTGAAGAAC ATCTTGGCGC TGAGGGGAGA CCCCGTGGGC 
GACCAGTGGG AAGTTGTGGA AGGAGGCTTC AGCTATGCCG CGGACTTGGT GAGGCACATC 
CGAAGCGAGT TTGGGGACTA CTTTGACATC TGTGTGGCAG GTTACCCCAA AGGCCACCCC 
GACGCAGGGA GCTTTGAGGC CGACCTGAAG TACTTGAAGG AGAAGGTATC CGCAGGAGCC 
GACTTCATCA TCACCCAGCT TTTCTTTGAG GCTGACACAT TCTTCCACTT TGTTAAGGCT 
TGCAGCGAGA TAGGCATAAC CTGCCCCATC CTCCCCGGAA TCTTTCCTAT TCAGGGCTAC 
CACTCCCTTC GGCAGCTCGT GAAACTGTCC AAGCTGGAGG TACCGCAGCA GATCAAAGAT 
GTGATTGAGC CAATCAAAGA CAATGATGCT GCGATCCGTA ACTATGGCAT CGAGCAGGCT 
GTGAGCCTGT GCCAGGAGCT TCTGACCAGT GGCTTGGTGC CAGGCCTCCA CTTCTACACC 
CTCAACCGCG AGATGGCCAC CACAGAGGTG CTGAAGCGCC TGGGCATGTG GATCGAGGAC 
CCCAGGCGTC CCCTACCCTG GGCTGTCAGT GCCCACCCCA AGCGCAGGGA GGAAGATGTA 
CGCCCCATTT TCTGGGCCTC CAGACCAAAG AGTTACATCT ACCGTACACA AGAGTGGGAT 
GAGTTCCCCA ATGGCCGCTG GGGCAACTCC TCCTCTCCAG CCTTCGGGGA GCTGAAGGAC 
TACTACCTCT TCTACCTGAA GAGCAAGTCC CCGAGGGAGG AGCTACTGAA GATGTGGGGG 
GAGGAGCTGA CCAGTGAAGA AAGCGTCTTT GAAGTCTTTG CCCGCTACCT CTCGGGAGAG 
CCCAACCAGC ACGGCTACAA GGTGACTTGC CTGCCCTGGA ATGACGAGCC CTTGGCGGCC 
GAGACCAGCC TGATGAAGGA GGAGCTGCTG CGAGTGAACC GGCAGGGCAT CCTCACCATC 
AACTCCCAGC CCAACATCAA CGGGAAACCA TCCTCTGACC CCATCGTGGG CTGGGGCCCC 
AGCGGGGGCT ATGTCTTCCA GAAGGCCTAC TTGGAGTTCT TCACTTCCCG CGAGACGGTG 
GGGGCGCTTC TGCAGGTGCT GAAGAAGTAT GAGCTCCGGG TTAATTACCA CATCGTGGAC 
GTGAAGGGTG AAAACATCAC CAATGCGCCT GAGCTGCAGC CCAATGCGGT CACTTGGGGC 
ATCTTCCCGG GGCGGGAGAT CATCCAGCCT ACGGTGGTAG ACCCTGTCAG CTTCATGTTC 
TGGAAGGATG AAGCCTTCGC CCTGTGGATC GAGCAGTGGG GCAAGCTGTA TGAGGAGGAG 
TCCCCGTCCC GCATGATCAT CCAGTACATC CACGACAACT ACTTCCTGGT CAACCTGGTG 
GACAATGAGT TTCCGCTGGA CAACTGCCTG TGGCAAGTGG TGGAAGACAC GTTTGAACTT 
CTCAAGGGGC CCTCACCAGA GCGAGAGGCA GAGGCCGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966939.1
CDS	153..1874
Translation	MGAGGPLFIDVTWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCCQSREQITGYLHKAKRLGLKNILALRGDPVGDQWEVVEGGFSYAADLVRHIRSEFGDYFDICVAGYPKGHPDAGSFEADLKYLKEKVSAGADFIITQLFFEADTFFHFVKACSEIGITCPILPGIFPIQGYHSLRQLVKLSKLEVPQQIKDVIEPIKDNDAAIRNYGIEQAVSLCQELLTSGLVPGLHFYTLNREMATTEVLKRLGMWIEDPRRPLPWAVSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFARYLSGEPNQHGYKVTCLPWNDEPLAAETSLMKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETVGALLQVLKKYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDNCLWQVVEDTFELLKGPSPEREAEAV

Target ORF information:

RefSeq Version	XM_014111464.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris methylenetetrahydrofolate reductase (MTHFR), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111464.2

ORF Insert Sequence:

ATGGGGGCAG GTGGCCCCCT CTTCATAGAT GTGACCTGGC ACCCGGCAGG GGACCCTGGC 
TCAGACAAGG AGACGTCCTC CATGATGATT GCCAGCACCG CCGTGAACTA CTGTGGCCTG 
GAGACCATCC TGCACATGAC CTGCTGCTGT CAGAGCCGGG AGCAGATCAC AGGCTATCTG 
CACAAGGCCA AGCGGCTGGG CCTGAAGAAC ATCTTGGCGC TGAGGGGAGA CCCCGTGGGC 
GACCAGTGGG AAGTTGTGGA AGGAGGCTTC AGCTATGCCG CGGACTTGGT GAGGCACATC 
CGAAGCGAGT TTGGGGACTA CTTTGACATC TGTGTGGCAG GTTACCCCAA AGGCCACCCC 
GACGCAGGGA GCTTTGAGGC CGACCTGAAG TACTTGAAGG AGAAGGTATC CGCAGGAGCC 
GACTTCATCA TCACCCAGCT TTTCTTTGAG GCTGACACAT TCTTCCACTT TGTTAAGGCT 
TGCAGCGAGA TAGGCATAAC CTGCCCCATC CTCCCCGGAA TCTTTCCTAT TCAGGGCTAC 
CACTCCCTTC GGCAGCTCGT GAAACTGTCC AAGCTGGAGG TACCGCAGCA GATCAAAGAT 
GTGATTGAGC CAATCAAAGA CAATGATGCT GCGATCCGTA ACTATGGCAT CGAGCAGGCT 
GTGAGCCTGT GCCAGGAGCT TCTGACCAGT GGCTTGGTGC CAGGCCTCCA CTTCTACACC 
CTCAACCGCG AGATGGCCAC CACAGAGGTG CTGAAGCGCC TGGGCATGTG GATCGAGGAC 
CCCAGGCGTC CCCTACCCTG GGCTGTCAGT GCCCACCCCA AGCGCAGGGA GGAAGATGTA 
CGCCCCATTT TCTGGGCCTC CAGACCAAAG AGTTACATCT ACCGTACACA AGAGTGGGAT 
GAGTTCCCCA ATGGCCGCTG GGGCAACTCC TCCTCTCCAG CCTTCGGGGA GCTGAAGGAC 
TACTACCTCT TCTACCTGAA GAGCAAGTCC CCGAGGGAGG AGCTACTGAA GATGTGGGGG 
GAGGAGCTGA CCAGTGAAGA AAGCGTCTTT GAAGTCTTTG CCCGCTACCT CTCGGGAGAG 
CCCAACCAGC ACGGCTACAA GGTGACTTGC CTGCCCTGGA ATGACGAGCC CTTGGCGGCC 
GAGACCAGCC TGATGAAGGA GGAGCTGCTG CGAGTGAACC GGCAGGGCAT CCTCACCATC 
AACTCCCAGC CCAACATCAA CGGGAAACCA TCCTCTGACC CCATCGTGGG CTGGGGCCCC 
AGCGGGGGCT ATGTCTTCCA GAAGGCCTAC TTGGAGTTCT TCACTTCCCG CGAGACGGTG 
GGGGCGCTTC TGCAGGTGCT GAAGAAGTAT GAGCTCCGGG TTAATTACCA CATCGTGGAC 
GTGAAGGGTG AAAACATCAC CAATGCGCCT GAGCTGCAGC CCAATGCGGT CACTTGGGGC 
ATCTTCCCGG GGCGGGAGAT CATCCAGCCT ACGGTGGTAG ACCCTGTCAG CTTCATGTTC 
TGGAAGGATG AAGCCTTCGC CCTGTGGATC GAGCAGTGGG GCAAGCTGTA TGAGGAGGAG 
TCCCCGTCCC GCATGATCAT CCAGTACATC CACGACAACT ACTTCCTGGT CAACCTGGTG 
GACAATGAGT TTCCGCTGGA CAACTGCCTG TGGCAAGTGG TGGAAGACAC GTTTGAACTT 
CTCAAGGGGC CCTCACCAGA GCGAGAGGCA GAGGCCGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MTHFR
Entrez Gene ID	478230
Full Name	methylenetetrahydrofolate reductase
General protein information	Preferred Names methylenetetrahydrofolate reductase Names methylenetetrahydrofolate reductase 5,10-methylenetetrahydrofolate reductase (NADPH)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Rattus norvegicus (Norway rat)	Mthfr	XP_342976.3
Danio rerio (zebrafish)	mthfr	NP_001121727.1
Kluyveromyces lactis	KLLA0F09647g	XP_455518.1
Magnaporthe oryzae (rice blast fungus)	MGG_01728	XP_003714725.1
Oryza sativa (rice)	Os03g0815200	NP_001051683.1
Mus musculus (house mouse)	Mthfr	NP_001155270.1
Bos taurus (cattle)	MTHFR	NP_001011685.1
Gallus gallus (chicken)	MTHFR	XP_417645.3
Caenorhabditis elegans (roundworm)	mthf-1	NP_741028.1
Arabidopsis thaliana (thale cress)	MTHFR2	NP_566011.1
Xenopus tropicalis (tropical clawed frog)	mthfr	NP_001096464.1
Homo sapiens (human)	MTHFR	NP_005948.3
Pan troglodytes (chimpanzee)	MTHFR	XP_001141040.1
	LOC714840	XP_001105017.1
Canis lupus familiaris (dog)	MTHFR	XP_005618049.1
Saccharomyces cerevisiae (baker's yeast)	MET13	NP_011390.2
Eremothecium gossypii	AGOS_AAR170W	NP_982713.2
Schizosaccharomyces pombe (fission yeast)	met9	NP_593224.1
Neurospora crassa	NCU07690	XP_961729.1
Arabidopsis thaliana (thale cress)	MTHFR1	NP_191556.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MTHFR cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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