Mthfr cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mthfr
Entrez Gene ID	17769
Full Name	methylenetetrahydrofolate reductase
Synonyms	AI323986
General protein information	Preferred Names methylenetetrahydrofolate reductase Names methylenetetrahydrofolate reductase 5,10-methylenetetrahydrofolate reductase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 E2\|4 78.67 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006538619.1	XP_006538682.1	methylenetetrahydrofolate reductase isoform X3
XM_006538622.3	XP_006538685.1	methylenetetrahydrofolate reductase isoform X3
XM_006538621.1	XP_006538684.1	methylenetetrahydrofolate reductase isoform X3
XM_006538618.3	XP_006538681.1	methylenetetrahydrofolate reductase isoform X2
XM_006538620.3	XP_006538683.1	methylenetetrahydrofolate reductase isoform X3
NM_010840.3	NP_034970.2	methylenetetrahydrofolate reductase isoform b
NM_001161798.1	NP_001155270.1	methylenetetrahydrofolate reductase isoform a
XM_006538617.3	XP_006538680.1	methylenetetrahydrofolate reductase isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mthfr	XP_342976.3
Danio rerio (zebrafish)	mthfr	NP_001121727.1
Kluyveromyces lactis	KLLA0F09647g	XP_455518.1
Magnaporthe oryzae (rice blast fungus)	MGG_01728	XP_003714725.1
Oryza sativa (rice)	Os03g0815200	NP_001051683.1
Mus musculus (house mouse)	Mthfr	NP_001155270.1
Bos taurus (cattle)	MTHFR	NP_001011685.1
Gallus gallus (chicken)	MTHFR	XP_417645.3
Caenorhabditis elegans (roundworm)	mthf-1	NP_741028.1
Arabidopsis thaliana (thale cress)	MTHFR2	NP_566011.1
Xenopus tropicalis (tropical clawed frog)	mthfr	NP_001096464.1
Homo sapiens (human)	MTHFR	NP_005948.3
Pan troglodytes (chimpanzee)	MTHFR	XP_001141040.1
	LOC714840	XP_001105017.1
Canis lupus familiaris (dog)	MTHFR	XP_005618049.1
Saccharomyces cerevisiae (baker's yeast)	MET13	NP_011390.2
Eremothecium gossypii	AGOS_AAR170W	NP_982713.2
Schizosaccharomyces pombe (fission yeast)	met9	NP_593224.1
Neurospora crassa	NCU07690	XP_961729.1
Arabidopsis thaliana (thale cress)	MTHFR1	NP_191556.1

Gene Ontology
Bibliography

Related articles in PubMed

Methylene-tetrahydrofolate reductase contributes to allergic airway disease.
Eyring KR, Pedersen BS, Maclean KN, Stabler SP, Yang IV, Schwartz DA
PloS one13(1)e0190916(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ganglion cell loss and vasculopathy observed in Mthfr+/- and Cbs+/- mouse retinas may be milder than expected, not because of compensatory increases of enzymes in remethylation/transsulfuration pathways, but because downstream transsulfuration pathway products GSH, taurine, and H2S are maintained at robust levels.
Title: Analysis of MTHFR, CBS, Glutathione, Taurine, and Hydrogen Sulfide Levels in Retinas of Hyperhomocysteinemic Mice.

In summary, high folate intake during pregnancy leads to pseudo-MTHFR deficiency, disturbed choline/methyl metabolism, embryonic growth delay and memory impairment in offspring. These findings highlight the unintended negative consequences of supplemental folic acid.
Title: High dietary folate in pregnant mice leads to pseudo-MTHFR deficiency and altered methyl metabolism, with embryonic growth delay and short-term memory impairment in offspring.

Study demonstrated that maternal MTHFR deficiency (i.e., in utero MTHFR deficiency) and early life exposure to vigabatrin separately and together alter the levels of proteins in the glutamatergic synapse
Title: Glutamatergic synapse protein composition of wild-type mice is sensitive to in utero MTHFR genotype and the timing of neonatal vigabatrin exposure.

Mildly hyperhomocysteinemic Mthfr+/- mice demonstrate reduced ganglion cell function, thinner NFL, and mild vasculopathy by 24 weeks.
Title: Retinal Ganglion Cell Loss and Mild Vasculopathy in Methylene Tetrahydrofolate Reductase (Mthfr)-Deficient Mice: A Model of Mild Hyperhomocysteinemia.

Data from Mthfr knockout mice (in homozygous/heterozygous matings) suggest that maternal genotype contributes to sensitivity to arsenic as embryotoxin (i.e., genetic predisposition to fetal resorption/congenital malformation in arsenic poisoning).
Title: Mthfr gene ablation enhances susceptibility to arsenic prenatal toxicity.

The following Mthfr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mthfr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu34038	XM_006538620.3 Latest version!	Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu34038	XM_006538619.1 Latest version!	Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu34038	XM_006538622.3 Latest version!	Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu34038	XM_006538621.1 Latest version!	Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu18963	NM_001161798.1 Latest version!	Mus musculus methylenetetrahydrofolate reductase (Mthfr), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu34036	XM_006538617.3 Latest version!	Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu34037	XM_006538618.3 Latest version!	Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu19413	NM_010840.3 Latest version!	Mus musculus methylenetetrahydrofolate reductase (Mthfr), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mthfr ( XM_006538619.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538682.1 Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu34038
Clone ID Related Accession (Same CDS sequence)	XM_006538619.1 , XM_006538622.3 , XM_006538621.1 , XM_006538620.3
Accession Version	XM_006538619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	methylenetetrahydrofolate reductase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACG AGGCCAGAGG AAGTGGCAGT CCCAACCCGC GATCTGAGGG CAGCAGCAGT 
GGCAGCGAGA GTTCCAAGGA CAGTTCAAGA TGTTCCACCC CCAGCCTGGA CCCAGAGCGG 
CACGAGAGAC TCCGGGAGAA GATGAGGCGC AGAATGGACT CTGGTGACAA GTGGTTCTCC 
CTGGAGTTCT TCCCCCCTCG AACTGCTGAG GGAGCTGTTA ACCTCATCTC CAGGTTTGAC 
CGGATGGCAG CAGGGGGCCC CCTCTTCGTA GATGTTACCT GGCACCCAGC TGGAGACCCT 
GGCTCAGACA AGGAGACCTC CTCCATGATG ATCGCCAGCA CAGCAGTAAA CTACTGCGGC 
TTGGAAACCA TCCTGCATAT GACCTGCTGC CAGCAGCGCC CGGAGGAGAT CACAGGCCAT 
CTGCACAGAG CCAAGCAGCT GGGCCTGAAG AACATAATGG CGCTGAGGGG AGACCCTGTA 
GGTGACCACT GGGAAGCAGA GGAAGGAGGC TTCAGCTATG CCACAGACCT GGTGAAGCAC 
ATCCGGACCG AGTTTGCTGA CTATTTTGAC ATCTGTGTGG CAGGTTACCC CAGAGGCCAC 
CCCGATGCAG AGAGCTTCGA GGATGACCTG AAGCATTTGA AGGAGAAGGT ATCTGCAGGC 
GCCGACTTCA TTATCACTCA GCTCTTCTTT GAGGCCAGCA CCTTCTTCAG CTTTGTGAAG 
GCCTGCACAG AGATAGGCAT CTCTTGCCCT ATCCTGCCTG GGATCTTCCC TATTCAGGGC 
TACACTTCCC TTCGGCAGCT TGTAAAACTG TCCAAGCTGG AGGTGCCACA GAAGATCAAG 
GATGTAATTG AGCCCATCAA AGACAACGAT GCTGCCATCC GCAACTACGG CATTGAGCTG 
GCTGTAAGCC TGTGCCGGGA GCTGCTGGAC AGTGGCTTGG TGCCAGGCCT CCACTTCTAT 
ACCCTCAACC GCGAGGTGGC CACCATGGAG GTGCTAAAGC AACTGGGCAT GTGGACCGAG 
GACCCCAGGC GTCCCTTGCC CTGGGCTCTC AGTGCGCATC CCAAGCGCCG GGAGGAAGAT 
GTCCGTCCCA TCTTCTGGGC CTCCAGACCA AAGAGCTACA TCTACCGCAC ACAGGACTGG 
GATGAGTTTC CTAACGGCCG CTGGGGTAAT TCTTCCTCAC CAGCCTTTGG GGAGCTGAAA 
GACTACTACC TCTTCTACCT GAAAAGCAAG TCCCCCAGGG AGGAGCTGCT GAAGATGTGG 
GGCGAGGAGC TCACCAGCGA AGAGAGTGTC TTTGAAGTCT TTGAACACTA CCTCTCTGGA 
GAGCCGAATC GCCATGGCTA CAGAGTGAGT GGGGTAACCT GCCTGCCCTG GAACGATGAA 
CCCCTGGCAG CGGAAACCAG CCTGATGAAG GAAGAGCTGC TCCGCGTGAA CAGGCTGGGC 
ATCCTCACCA TCAACTCTCA GCCCAACATC AACGCAAAAC CATCCTCAGA CCCTGTTGTG 
GGCTGGGGCC CCAGTGGGGG TTATGTCTTC CAGAAGGCCT ACCTCGAATT CTTCACCTCC 
CGTGAAACTG TGGAGGCGCT TCTGCAGGTG CTGAAGACAT ACGAGCTGCG GGTCAACTAC 
CACATCGTGG ACGTGAAGGG AGAGAACATC ACTAATGCCC CTGAGCTGCA GCCCAATGCC 
GTGACGTGGG GCATCTTCCC GGGTCGAGAG ATCATCCAGC CTACTGTGGT GGACCCCATC 
AGCTTCATGT TCTGGAAGGA TGAGGCCTTT GCCCTGTGGA TCGAGCAGTG GGGCAAGCTA 
TACGAGGAGG AGTCGCCATC CCGCATGATC ATCCAATACA TCCATGACAA CTATTTCCTG 
GTCAACCTGG TGGACAACGA GTTCCCGCTG GACAGCTGCC TGTGGCAGGT GGTGGAGGAC 
ACGTTTGAGC TGCTCAACAG GCATCCCACG GAGAGAGAGA CACAGGCTCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538682.1
CDS	294..2267
Translation	MVNEARGSGSPNPRSEGSSSGSESSKDSSRCSTPSLDPERHERLREKMRRRMDSGDKWFSLEFFPPRTAEGAVNLISRFDRMAAGGPLFVDVTWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCQQRPEEITGHLHRAKQLGLKNIMALRGDPVGDHWEAEEGGFSYATDLVKHIRTEFADYFDICVAGYPRGHPDAESFEDDLKHLKEKVSAGADFIITQLFFEASTFFSFVKACTEIGISCPILPGIFPIQGYTSLRQLVKLSKLEVPQKIKDVIEPIKDNDAAIRNYGIELAVSLCRELLDSGLVPGLHFYTLNREVATMEVLKQLGMWTEDPRRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQDWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFEHYLSGEPNRHGYRVSGVTCLPWNDEPLAAETSLMKEELLRVNRLGILTINSQPNINAKPSSDPVVGWGPSGGYVFQKAYLEFFTSRETVEALLQVLKTYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPISFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDSCLWQVVEDTFELLNRHPTERETQAP

Target ORF information:

RefSeq Version	XM_006538619.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538619.1

ORF Insert Sequence:

ATGGTGAACG AGGCCAGAGG AAGTGGCAGT CCCAACCCGC GATCTGAGGG CAGCAGCAGT 
GGCAGCGAGA GTTCCAAGGA CAGTTCAAGA TGTTCCACCC CCAGCCTGGA CCCAGAGCGG 
CACGAGAGAC TCCGGGAGAA GATGAGGCGC AGAATGGACT CTGGTGACAA GTGGTTCTCC 
CTGGAGTTCT TCCCCCCTCG AACTGCTGAG GGAGCTGTTA ACCTCATCTC CAGGTTTGAC 
CGGATGGCAG CAGGGGGCCC CCTCTTCGTA GATGTTACCT GGCACCCAGC TGGAGACCCT 
GGCTCAGACA AGGAGACCTC CTCCATGATG ATCGCCAGCA CAGCAGTAAA CTACTGCGGC 
TTGGAAACCA TCCTGCATAT GACCTGCTGC CAGCAGCGCC CGGAGGAGAT CACAGGCCAT 
CTGCACAGAG CCAAGCAGCT GGGCCTGAAG AACATAATGG CGCTGAGGGG AGACCCTGTA 
GGTGACCACT GGGAAGCAGA GGAAGGAGGC TTCAGCTATG CCACAGACCT GGTGAAGCAC 
ATCCGGACCG AGTTTGCTGA CTATTTTGAC ATCTGTGTGG CAGGTTACCC CAGAGGCCAC 
CCCGATGCAG AGAGCTTCGA GGATGACCTG AAGCATTTGA AGGAGAAGGT ATCTGCAGGC 
GCCGACTTCA TTATCACTCA GCTCTTCTTT GAGGCCAGCA CCTTCTTCAG CTTTGTGAAG 
GCCTGCACAG AGATAGGCAT CTCTTGCCCT ATCCTGCCTG GGATCTTCCC TATTCAGGGC 
TACACTTCCC TTCGGCAGCT TGTAAAACTG TCCAAGCTGG AGGTGCCACA GAAGATCAAG 
GATGTAATTG AGCCCATCAA AGACAACGAT GCTGCCATCC GCAACTACGG CATTGAGCTG 
GCTGTAAGCC TGTGCCGGGA GCTGCTGGAC AGTGGCTTGG TGCCAGGCCT CCACTTCTAT 
ACCCTCAACC GCGAGGTGGC CACCATGGAG GTGCTAAAGC AACTGGGCAT GTGGACCGAG 
GACCCCAGGC GTCCCTTGCC CTGGGCTCTC AGTGCGCATC CCAAGCGCCG GGAGGAAGAT 
GTCCGTCCCA TCTTCTGGGC CTCCAGACCA AAGAGCTACA TCTACCGCAC ACAGGACTGG 
GATGAGTTTC CTAACGGCCG CTGGGGTAAT TCTTCCTCAC CAGCCTTTGG GGAGCTGAAA 
GACTACTACC TCTTCTACCT GAAAAGCAAG TCCCCCAGGG AGGAGCTGCT GAAGATGTGG 
GGCGAGGAGC TCACCAGCGA AGAGAGTGTC TTTGAAGTCT TTGAACACTA CCTCTCTGGA 
GAGCCGAATC GCCATGGCTA CAGAGTGAGT GGGGTAACCT GCCTGCCCTG GAACGATGAA 
CCCCTGGCAG CGGAAACCAG CCTGATGAAG GAAGAGCTGC TCCGCGTGAA CAGGCTGGGC 
ATCCTCACCA TCAACTCTCA GCCCAACATC AACGCAAAAC CATCCTCAGA CCCTGTTGTG 
GGCTGGGGCC CCAGTGGGGG TTATGTCTTC CAGAAGGCCT ACCTCGAATT CTTCACCTCC 
CGTGAAACTG TGGAGGCGCT TCTGCAGGTG CTGAAGACAT ACGAGCTGCG GGTCAACTAC 
CACATCGTGG ACGTGAAGGG AGAGAACATC ACTAATGCCC CTGAGCTGCA GCCCAATGCC 
GTGACGTGGG GCATCTTCCC GGGTCGAGAG ATCATCCAGC CTACTGTGGT GGACCCCATC 
AGCTTCATGT TCTGGAAGGA TGAGGCCTTT GCCCTGTGGA TCGAGCAGTG GGGCAAGCTA 
TACGAGGAGG AGTCGCCATC CCGCATGATC ATCCAATACA TCCATGACAA CTATTTCCTG 
GTCAACCTGG TGGACAACGA GTTCCCGCTG GACAGCTGCC TGTGGCAGGT GGTGGAGGAC 
ACGTTTGAGC TGCTCAACAG GCATCCCACG GAGAGAGAGA CACAGGCTCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mthfr ( XM_006538622.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538685.1 Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu34038
Clone ID Related Accession (Same CDS sequence)	XM_006538619.1 , XM_006538622.3 , XM_006538621.1 , XM_006538620.3
Accession Version	XM_006538622.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	methylenetetrahydrofolate reductase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538622.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACG AGGCCAGAGG AAGTGGCAGT CCCAACCCGC GATCTGAGGG CAGCAGCAGT 
GGCAGCGAGA GTTCCAAGGA CAGTTCAAGA TGTTCCACCC CCAGCCTGGA CCCAGAGCGG 
CACGAGAGAC TCCGGGAGAA GATGAGGCGC AGAATGGACT CTGGTGACAA GTGGTTCTCC 
CTGGAGTTCT TCCCCCCTCG AACTGCTGAG GGAGCTGTTA ACCTCATCTC CAGGTTTGAC 
CGGATGGCAG CAGGGGGCCC CCTCTTCGTA GATGTTACCT GGCACCCAGC TGGAGACCCT 
GGCTCAGACA AGGAGACCTC CTCCATGATG ATCGCCAGCA CAGCAGTAAA CTACTGCGGC 
TTGGAAACCA TCCTGCATAT GACCTGCTGC CAGCAGCGCC CGGAGGAGAT CACAGGCCAT 
CTGCACAGAG CCAAGCAGCT GGGCCTGAAG AACATAATGG CGCTGAGGGG AGACCCTGTA 
GGTGACCACT GGGAAGCAGA GGAAGGAGGC TTCAGCTATG CCACAGACCT GGTGAAGCAC 
ATCCGGACCG AGTTTGCTGA CTATTTTGAC ATCTGTGTGG CAGGTTACCC CAGAGGCCAC 
CCCGATGCAG AGAGCTTCGA GGATGACCTG AAGCATTTGA AGGAGAAGGT ATCTGCAGGC 
GCCGACTTCA TTATCACTCA GCTCTTCTTT GAGGCCAGCA CCTTCTTCAG CTTTGTGAAG 
GCCTGCACAG AGATAGGCAT CTCTTGCCCT ATCCTGCCTG GGATCTTCCC TATTCAGGGC 
TACACTTCCC TTCGGCAGCT TGTAAAACTG TCCAAGCTGG AGGTGCCACA GAAGATCAAG 
GATGTAATTG AGCCCATCAA AGACAACGAT GCTGCCATCC GCAACTACGG CATTGAGCTG 
GCTGTAAGCC TGTGCCGGGA GCTGCTGGAC AGTGGCTTGG TGCCAGGCCT CCACTTCTAT 
ACCCTCAACC GCGAGGTGGC CACCATGGAG GTGCTAAAGC AACTGGGCAT GTGGACCGAG 
GACCCCAGGC GTCCCTTGCC CTGGGCTCTC AGTGCGCATC CCAAGCGCCG GGAGGAAGAT 
GTCCGTCCCA TCTTCTGGGC CTCCAGACCA AAGAGCTACA TCTACCGCAC ACAGGACTGG 
GATGAGTTTC CTAACGGCCG CTGGGGTAAT TCTTCCTCAC CAGCCTTTGG GGAGCTGAAA 
GACTACTACC TCTTCTACCT GAAAAGCAAG TCCCCCAGGG AGGAGCTGCT GAAGATGTGG 
GGCGAGGAGC TCACCAGCGA AGAGAGTGTC TTTGAAGTCT TTGAACACTA CCTCTCTGGA 
GAGCCGAATC GCCATGGCTA CAGAGTGAGT GGGGTAACCT GCCTGCCCTG GAACGATGAA 
CCCCTGGCAG CGGAAACCAG CCTGATGAAG GAAGAGCTGC TCCGCGTGAA CAGGCTGGGC 
ATCCTCACCA TCAACTCTCA GCCCAACATC AACGCAAAAC CATCCTCAGA CCCTGTTGTG 
GGCTGGGGCC CCAGTGGGGG TTATGTCTTC CAGAAGGCCT ACCTCGAATT CTTCACCTCC 
CGTGAAACTG TGGAGGCGCT TCTGCAGGTG CTGAAGACAT ACGAGCTGCG GGTCAACTAC 
CACATCGTGG ACGTGAAGGG AGAGAACATC ACTAATGCCC CTGAGCTGCA GCCCAATGCC 
GTGACGTGGG GCATCTTCCC GGGTCGAGAG ATCATCCAGC CTACTGTGGT GGACCCCATC 
AGCTTCATGT TCTGGAAGGA TGAGGCCTTT GCCCTGTGGA TCGAGCAGTG GGGCAAGCTA 
TACGAGGAGG AGTCGCCATC CCGCATGATC ATCCAATACA TCCATGACAA CTATTTCCTG 
GTCAACCTGG TGGACAACGA GTTCCCGCTG GACAGCTGCC TGTGGCAGGT GGTGGAGGAC 
ACGTTTGAGC TGCTCAACAG GCATCCCACG GAGAGAGAGA CACAGGCTCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538685.1
CDS	232..2205
Translation	MVNEARGSGSPNPRSEGSSSGSESSKDSSRCSTPSLDPERHERLREKMRRRMDSGDKWFSLEFFPPRTAEGAVNLISRFDRMAAGGPLFVDVTWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCQQRPEEITGHLHRAKQLGLKNIMALRGDPVGDHWEAEEGGFSYATDLVKHIRTEFADYFDICVAGYPRGHPDAESFEDDLKHLKEKVSAGADFIITQLFFEASTFFSFVKACTEIGISCPILPGIFPIQGYTSLRQLVKLSKLEVPQKIKDVIEPIKDNDAAIRNYGIELAVSLCRELLDSGLVPGLHFYTLNREVATMEVLKQLGMWTEDPRRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQDWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFEHYLSGEPNRHGYRVSGVTCLPWNDEPLAAETSLMKEELLRVNRLGILTINSQPNINAKPSSDPVVGWGPSGGYVFQKAYLEFFTSRETVEALLQVLKTYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPISFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDSCLWQVVEDTFELLNRHPTERETQAP

Target ORF information:

RefSeq Version	XM_006538622.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538622.3

ORF Insert Sequence:

ATGGTGAACG AGGCCAGAGG AAGTGGCAGT CCCAACCCGC GATCTGAGGG CAGCAGCAGT 
GGCAGCGAGA GTTCCAAGGA CAGTTCAAGA TGTTCCACCC CCAGCCTGGA CCCAGAGCGG 
CACGAGAGAC TCCGGGAGAA GATGAGGCGC AGAATGGACT CTGGTGACAA GTGGTTCTCC 
CTGGAGTTCT TCCCCCCTCG AACTGCTGAG GGAGCTGTTA ACCTCATCTC CAGGTTTGAC 
CGGATGGCAG CAGGGGGCCC CCTCTTCGTA GATGTTACCT GGCACCCAGC TGGAGACCCT 
GGCTCAGACA AGGAGACCTC CTCCATGATG ATCGCCAGCA CAGCAGTAAA CTACTGCGGC 
TTGGAAACCA TCCTGCATAT GACCTGCTGC CAGCAGCGCC CGGAGGAGAT CACAGGCCAT 
CTGCACAGAG CCAAGCAGCT GGGCCTGAAG AACATAATGG CGCTGAGGGG AGACCCTGTA 
GGTGACCACT GGGAAGCAGA GGAAGGAGGC TTCAGCTATG CCACAGACCT GGTGAAGCAC 
ATCCGGACCG AGTTTGCTGA CTATTTTGAC ATCTGTGTGG CAGGTTACCC CAGAGGCCAC 
CCCGATGCAG AGAGCTTCGA GGATGACCTG AAGCATTTGA AGGAGAAGGT ATCTGCAGGC 
GCCGACTTCA TTATCACTCA GCTCTTCTTT GAGGCCAGCA CCTTCTTCAG CTTTGTGAAG 
GCCTGCACAG AGATAGGCAT CTCTTGCCCT ATCCTGCCTG GGATCTTCCC TATTCAGGGC 
TACACTTCCC TTCGGCAGCT TGTAAAACTG TCCAAGCTGG AGGTGCCACA GAAGATCAAG 
GATGTAATTG AGCCCATCAA AGACAACGAT GCTGCCATCC GCAACTACGG CATTGAGCTG 
GCTGTAAGCC TGTGCCGGGA GCTGCTGGAC AGTGGCTTGG TGCCAGGCCT CCACTTCTAT 
ACCCTCAACC GCGAGGTGGC CACCATGGAG GTGCTAAAGC AACTGGGCAT GTGGACCGAG 
GACCCCAGGC GTCCCTTGCC CTGGGCTCTC AGTGCGCATC CCAAGCGCCG GGAGGAAGAT 
GTCCGTCCCA TCTTCTGGGC CTCCAGACCA AAGAGCTACA TCTACCGCAC ACAGGACTGG 
GATGAGTTTC CTAACGGCCG CTGGGGTAAT TCTTCCTCAC CAGCCTTTGG GGAGCTGAAA 
GACTACTACC TCTTCTACCT GAAAAGCAAG TCCCCCAGGG AGGAGCTGCT GAAGATGTGG 
GGCGAGGAGC TCACCAGCGA AGAGAGTGTC TTTGAAGTCT TTGAACACTA CCTCTCTGGA 
GAGCCGAATC GCCATGGCTA CAGAGTGAGT GGGGTAACCT GCCTGCCCTG GAACGATGAA 
CCCCTGGCAG CGGAAACCAG CCTGATGAAG GAAGAGCTGC TCCGCGTGAA CAGGCTGGGC 
ATCCTCACCA TCAACTCTCA GCCCAACATC AACGCAAAAC CATCCTCAGA CCCTGTTGTG 
GGCTGGGGCC CCAGTGGGGG TTATGTCTTC CAGAAGGCCT ACCTCGAATT CTTCACCTCC 
CGTGAAACTG TGGAGGCGCT TCTGCAGGTG CTGAAGACAT ACGAGCTGCG GGTCAACTAC 
CACATCGTGG ACGTGAAGGG AGAGAACATC ACTAATGCCC CTGAGCTGCA GCCCAATGCC 
GTGACGTGGG GCATCTTCCC GGGTCGAGAG ATCATCCAGC CTACTGTGGT GGACCCCATC 
AGCTTCATGT TCTGGAAGGA TGAGGCCTTT GCCCTGTGGA TCGAGCAGTG GGGCAAGCTA 
TACGAGGAGG AGTCGCCATC CCGCATGATC ATCCAATACA TCCATGACAA CTATTTCCTG 
GTCAACCTGG TGGACAACGA GTTCCCGCTG GACAGCTGCC TGTGGCAGGT GGTGGAGGAC 
ACGTTTGAGC TGCTCAACAG GCATCCCACG GAGAGAGAGA CACAGGCTCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mthfr ( XM_006538621.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538684.1 Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X8, mRNA.

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CloneID	OMu34038
Clone ID Related Accession (Same CDS sequence)	XM_006538619.1 , XM_006538622.3 , XM_006538621.1 , XM_006538620.3
Accession Version	XM_006538621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	methylenetetrahydrofolate reductase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACG AGGCCAGAGG AAGTGGCAGT CCCAACCCGC GATCTGAGGG CAGCAGCAGT 
GGCAGCGAGA GTTCCAAGGA CAGTTCAAGA TGTTCCACCC CCAGCCTGGA CCCAGAGCGG 
CACGAGAGAC TCCGGGAGAA GATGAGGCGC AGAATGGACT CTGGTGACAA GTGGTTCTCC 
CTGGAGTTCT TCCCCCCTCG AACTGCTGAG GGAGCTGTTA ACCTCATCTC CAGGTTTGAC 
CGGATGGCAG CAGGGGGCCC CCTCTTCGTA GATGTTACCT GGCACCCAGC TGGAGACCCT 
GGCTCAGACA AGGAGACCTC CTCCATGATG ATCGCCAGCA CAGCAGTAAA CTACTGCGGC 
TTGGAAACCA TCCTGCATAT GACCTGCTGC CAGCAGCGCC CGGAGGAGAT CACAGGCCAT 
CTGCACAGAG CCAAGCAGCT GGGCCTGAAG AACATAATGG CGCTGAGGGG AGACCCTGTA 
GGTGACCACT GGGAAGCAGA GGAAGGAGGC TTCAGCTATG CCACAGACCT GGTGAAGCAC 
ATCCGGACCG AGTTTGCTGA CTATTTTGAC ATCTGTGTGG CAGGTTACCC CAGAGGCCAC 
CCCGATGCAG AGAGCTTCGA GGATGACCTG AAGCATTTGA AGGAGAAGGT ATCTGCAGGC 
GCCGACTTCA TTATCACTCA GCTCTTCTTT GAGGCCAGCA CCTTCTTCAG CTTTGTGAAG 
GCCTGCACAG AGATAGGCAT CTCTTGCCCT ATCCTGCCTG GGATCTTCCC TATTCAGGGC 
TACACTTCCC TTCGGCAGCT TGTAAAACTG TCCAAGCTGG AGGTGCCACA GAAGATCAAG 
GATGTAATTG AGCCCATCAA AGACAACGAT GCTGCCATCC GCAACTACGG CATTGAGCTG 
GCTGTAAGCC TGTGCCGGGA GCTGCTGGAC AGTGGCTTGG TGCCAGGCCT CCACTTCTAT 
ACCCTCAACC GCGAGGTGGC CACCATGGAG GTGCTAAAGC AACTGGGCAT GTGGACCGAG 
GACCCCAGGC GTCCCTTGCC CTGGGCTCTC AGTGCGCATC CCAAGCGCCG GGAGGAAGAT 
GTCCGTCCCA TCTTCTGGGC CTCCAGACCA AAGAGCTACA TCTACCGCAC ACAGGACTGG 
GATGAGTTTC CTAACGGCCG CTGGGGTAAT TCTTCCTCAC CAGCCTTTGG GGAGCTGAAA 
GACTACTACC TCTTCTACCT GAAAAGCAAG TCCCCCAGGG AGGAGCTGCT GAAGATGTGG 
GGCGAGGAGC TCACCAGCGA AGAGAGTGTC TTTGAAGTCT TTGAACACTA CCTCTCTGGA 
GAGCCGAATC GCCATGGCTA CAGAGTGAGT GGGGTAACCT GCCTGCCCTG GAACGATGAA 
CCCCTGGCAG CGGAAACCAG CCTGATGAAG GAAGAGCTGC TCCGCGTGAA CAGGCTGGGC 
ATCCTCACCA TCAACTCTCA GCCCAACATC AACGCAAAAC CATCCTCAGA CCCTGTTGTG 
GGCTGGGGCC CCAGTGGGGG TTATGTCTTC CAGAAGGCCT ACCTCGAATT CTTCACCTCC 
CGTGAAACTG TGGAGGCGCT TCTGCAGGTG CTGAAGACAT ACGAGCTGCG GGTCAACTAC 
CACATCGTGG ACGTGAAGGG AGAGAACATC ACTAATGCCC CTGAGCTGCA GCCCAATGCC 
GTGACGTGGG GCATCTTCCC GGGTCGAGAG ATCATCCAGC CTACTGTGGT GGACCCCATC 
AGCTTCATGT TCTGGAAGGA TGAGGCCTTT GCCCTGTGGA TCGAGCAGTG GGGCAAGCTA 
TACGAGGAGG AGTCGCCATC CCGCATGATC ATCCAATACA TCCATGACAA CTATTTCCTG 
GTCAACCTGG TGGACAACGA GTTCCCGCTG GACAGCTGCC TGTGGCAGGT GGTGGAGGAC 
ACGTTTGAGC TGCTCAACAG GCATCCCACG GAGAGAGAGA CACAGGCTCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538684.1
CDS	282..2255
Translation	MVNEARGSGSPNPRSEGSSSGSESSKDSSRCSTPSLDPERHERLREKMRRRMDSGDKWFSLEFFPPRTAEGAVNLISRFDRMAAGGPLFVDVTWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCQQRPEEITGHLHRAKQLGLKNIMALRGDPVGDHWEAEEGGFSYATDLVKHIRTEFADYFDICVAGYPRGHPDAESFEDDLKHLKEKVSAGADFIITQLFFEASTFFSFVKACTEIGISCPILPGIFPIQGYTSLRQLVKLSKLEVPQKIKDVIEPIKDNDAAIRNYGIELAVSLCRELLDSGLVPGLHFYTLNREVATMEVLKQLGMWTEDPRRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQDWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFEHYLSGEPNRHGYRVSGVTCLPWNDEPLAAETSLMKEELLRVNRLGILTINSQPNINAKPSSDPVVGWGPSGGYVFQKAYLEFFTSRETVEALLQVLKTYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPISFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDSCLWQVVEDTFELLNRHPTERETQAP

Target ORF information:

RefSeq Version	XM_006538621.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538621.1

ORF Insert Sequence:

ATGGTGAACG AGGCCAGAGG AAGTGGCAGT CCCAACCCGC GATCTGAGGG CAGCAGCAGT 
GGCAGCGAGA GTTCCAAGGA CAGTTCAAGA TGTTCCACCC CCAGCCTGGA CCCAGAGCGG 
CACGAGAGAC TCCGGGAGAA GATGAGGCGC AGAATGGACT CTGGTGACAA GTGGTTCTCC 
CTGGAGTTCT TCCCCCCTCG AACTGCTGAG GGAGCTGTTA ACCTCATCTC CAGGTTTGAC 
CGGATGGCAG CAGGGGGCCC CCTCTTCGTA GATGTTACCT GGCACCCAGC TGGAGACCCT 
GGCTCAGACA AGGAGACCTC CTCCATGATG ATCGCCAGCA CAGCAGTAAA CTACTGCGGC 
TTGGAAACCA TCCTGCATAT GACCTGCTGC CAGCAGCGCC CGGAGGAGAT CACAGGCCAT 
CTGCACAGAG CCAAGCAGCT GGGCCTGAAG AACATAATGG CGCTGAGGGG AGACCCTGTA 
GGTGACCACT GGGAAGCAGA GGAAGGAGGC TTCAGCTATG CCACAGACCT GGTGAAGCAC 
ATCCGGACCG AGTTTGCTGA CTATTTTGAC ATCTGTGTGG CAGGTTACCC CAGAGGCCAC 
CCCGATGCAG AGAGCTTCGA GGATGACCTG AAGCATTTGA AGGAGAAGGT ATCTGCAGGC 
GCCGACTTCA TTATCACTCA GCTCTTCTTT GAGGCCAGCA CCTTCTTCAG CTTTGTGAAG 
GCCTGCACAG AGATAGGCAT CTCTTGCCCT ATCCTGCCTG GGATCTTCCC TATTCAGGGC 
TACACTTCCC TTCGGCAGCT TGTAAAACTG TCCAAGCTGG AGGTGCCACA GAAGATCAAG 
GATGTAATTG AGCCCATCAA AGACAACGAT GCTGCCATCC GCAACTACGG CATTGAGCTG 
GCTGTAAGCC TGTGCCGGGA GCTGCTGGAC AGTGGCTTGG TGCCAGGCCT CCACTTCTAT 
ACCCTCAACC GCGAGGTGGC CACCATGGAG GTGCTAAAGC AACTGGGCAT GTGGACCGAG 
GACCCCAGGC GTCCCTTGCC CTGGGCTCTC AGTGCGCATC CCAAGCGCCG GGAGGAAGAT 
GTCCGTCCCA TCTTCTGGGC CTCCAGACCA AAGAGCTACA TCTACCGCAC ACAGGACTGG 
GATGAGTTTC CTAACGGCCG CTGGGGTAAT TCTTCCTCAC CAGCCTTTGG GGAGCTGAAA 
GACTACTACC TCTTCTACCT GAAAAGCAAG TCCCCCAGGG AGGAGCTGCT GAAGATGTGG 
GGCGAGGAGC TCACCAGCGA AGAGAGTGTC TTTGAAGTCT TTGAACACTA CCTCTCTGGA 
GAGCCGAATC GCCATGGCTA CAGAGTGAGT GGGGTAACCT GCCTGCCCTG GAACGATGAA 
CCCCTGGCAG CGGAAACCAG CCTGATGAAG GAAGAGCTGC TCCGCGTGAA CAGGCTGGGC 
ATCCTCACCA TCAACTCTCA GCCCAACATC AACGCAAAAC CATCCTCAGA CCCTGTTGTG 
GGCTGGGGCC CCAGTGGGGG TTATGTCTTC CAGAAGGCCT ACCTCGAATT CTTCACCTCC 
CGTGAAACTG TGGAGGCGCT TCTGCAGGTG CTGAAGACAT ACGAGCTGCG GGTCAACTAC 
CACATCGTGG ACGTGAAGGG AGAGAACATC ACTAATGCCC CTGAGCTGCA GCCCAATGCC 
GTGACGTGGG GCATCTTCCC GGGTCGAGAG ATCATCCAGC CTACTGTGGT GGACCCCATC 
AGCTTCATGT TCTGGAAGGA TGAGGCCTTT GCCCTGTGGA TCGAGCAGTG GGGCAAGCTA 
TACGAGGAGG AGTCGCCATC CCGCATGATC ATCCAATACA TCCATGACAA CTATTTCCTG 
GTCAACCTGG TGGACAACGA GTTCCCGCTG GACAGCTGCC TGTGGCAGGT GGTGGAGGAC 
ACGTTTGAGC TGCTCAACAG GCATCCCACG GAGAGAGAGA CACAGGCTCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mthfr ( XM_006538620.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538683.1 Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X7, mRNA.

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CloneID	OMu34038
Clone ID Related Accession (Same CDS sequence)	XM_006538619.1 , XM_006538622.3 , XM_006538621.1 , XM_006538620.3
Accession Version	XM_006538620.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	methylenetetrahydrofolate reductase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538620.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACG AGGCCAGAGG AAGTGGCAGT CCCAACCCGC GATCTGAGGG CAGCAGCAGT 
GGCAGCGAGA GTTCCAAGGA CAGTTCAAGA TGTTCCACCC CCAGCCTGGA CCCAGAGCGG 
CACGAGAGAC TCCGGGAGAA GATGAGGCGC AGAATGGACT CTGGTGACAA GTGGTTCTCC 
CTGGAGTTCT TCCCCCCTCG AACTGCTGAG GGAGCTGTTA ACCTCATCTC CAGGTTTGAC 
CGGATGGCAG CAGGGGGCCC CCTCTTCGTA GATGTTACCT GGCACCCAGC TGGAGACCCT 
GGCTCAGACA AGGAGACCTC CTCCATGATG ATCGCCAGCA CAGCAGTAAA CTACTGCGGC 
TTGGAAACCA TCCTGCATAT GACCTGCTGC CAGCAGCGCC CGGAGGAGAT CACAGGCCAT 
CTGCACAGAG CCAAGCAGCT GGGCCTGAAG AACATAATGG CGCTGAGGGG AGACCCTGTA 
GGTGACCACT GGGAAGCAGA GGAAGGAGGC TTCAGCTATG CCACAGACCT GGTGAAGCAC 
ATCCGGACCG AGTTTGCTGA CTATTTTGAC ATCTGTGTGG CAGGTTACCC CAGAGGCCAC 
CCCGATGCAG AGAGCTTCGA GGATGACCTG AAGCATTTGA AGGAGAAGGT ATCTGCAGGC 
GCCGACTTCA TTATCACTCA GCTCTTCTTT GAGGCCAGCA CCTTCTTCAG CTTTGTGAAG 
GCCTGCACAG AGATAGGCAT CTCTTGCCCT ATCCTGCCTG GGATCTTCCC TATTCAGGGC 
TACACTTCCC TTCGGCAGCT TGTAAAACTG TCCAAGCTGG AGGTGCCACA GAAGATCAAG 
GATGTAATTG AGCCCATCAA AGACAACGAT GCTGCCATCC GCAACTACGG CATTGAGCTG 
GCTGTAAGCC TGTGCCGGGA GCTGCTGGAC AGTGGCTTGG TGCCAGGCCT CCACTTCTAT 
ACCCTCAACC GCGAGGTGGC CACCATGGAG GTGCTAAAGC AACTGGGCAT GTGGACCGAG 
GACCCCAGGC GTCCCTTGCC CTGGGCTCTC AGTGCGCATC CCAAGCGCCG GGAGGAAGAT 
GTCCGTCCCA TCTTCTGGGC CTCCAGACCA AAGAGCTACA TCTACCGCAC ACAGGACTGG 
GATGAGTTTC CTAACGGCCG CTGGGGTAAT TCTTCCTCAC CAGCCTTTGG GGAGCTGAAA 
GACTACTACC TCTTCTACCT GAAAAGCAAG TCCCCCAGGG AGGAGCTGCT GAAGATGTGG 
GGCGAGGAGC TCACCAGCGA AGAGAGTGTC TTTGAAGTCT TTGAACACTA CCTCTCTGGA 
GAGCCGAATC GCCATGGCTA CAGAGTGAGT GGGGTAACCT GCCTGCCCTG GAACGATGAA 
CCCCTGGCAG CGGAAACCAG CCTGATGAAG GAAGAGCTGC TCCGCGTGAA CAGGCTGGGC 
ATCCTCACCA TCAACTCTCA GCCCAACATC AACGCAAAAC CATCCTCAGA CCCTGTTGTG 
GGCTGGGGCC CCAGTGGGGG TTATGTCTTC CAGAAGGCCT ACCTCGAATT CTTCACCTCC 
CGTGAAACTG TGGAGGCGCT TCTGCAGGTG CTGAAGACAT ACGAGCTGCG GGTCAACTAC 
CACATCGTGG ACGTGAAGGG AGAGAACATC ACTAATGCCC CTGAGCTGCA GCCCAATGCC 
GTGACGTGGG GCATCTTCCC GGGTCGAGAG ATCATCCAGC CTACTGTGGT GGACCCCATC 
AGCTTCATGT TCTGGAAGGA TGAGGCCTTT GCCCTGTGGA TCGAGCAGTG GGGCAAGCTA 
TACGAGGAGG AGTCGCCATC CCGCATGATC ATCCAATACA TCCATGACAA CTATTTCCTG 
GTCAACCTGG TGGACAACGA GTTCCCGCTG GACAGCTGCC TGTGGCAGGT GGTGGAGGAC 
ACGTTTGAGC TGCTCAACAG GCATCCCACG GAGAGAGAGA CACAGGCTCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538683.1
CDS	235..2208
Translation	MVNEARGSGSPNPRSEGSSSGSESSKDSSRCSTPSLDPERHERLREKMRRRMDSGDKWFSLEFFPPRTAEGAVNLISRFDRMAAGGPLFVDVTWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCQQRPEEITGHLHRAKQLGLKNIMALRGDPVGDHWEAEEGGFSYATDLVKHIRTEFADYFDICVAGYPRGHPDAESFEDDLKHLKEKVSAGADFIITQLFFEASTFFSFVKACTEIGISCPILPGIFPIQGYTSLRQLVKLSKLEVPQKIKDVIEPIKDNDAAIRNYGIELAVSLCRELLDSGLVPGLHFYTLNREVATMEVLKQLGMWTEDPRRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQDWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFEHYLSGEPNRHGYRVSGVTCLPWNDEPLAAETSLMKEELLRVNRLGILTINSQPNINAKPSSDPVVGWGPSGGYVFQKAYLEFFTSRETVEALLQVLKTYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPISFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDSCLWQVVEDTFELLNRHPTERETQAP

Target ORF information:

RefSeq Version	XM_006538620.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538620.3

ORF Insert Sequence:

ATGGTGAACG AGGCCAGAGG AAGTGGCAGT CCCAACCCGC GATCTGAGGG CAGCAGCAGT 
GGCAGCGAGA GTTCCAAGGA CAGTTCAAGA TGTTCCACCC CCAGCCTGGA CCCAGAGCGG 
CACGAGAGAC TCCGGGAGAA GATGAGGCGC AGAATGGACT CTGGTGACAA GTGGTTCTCC 
CTGGAGTTCT TCCCCCCTCG AACTGCTGAG GGAGCTGTTA ACCTCATCTC CAGGTTTGAC 
CGGATGGCAG CAGGGGGCCC CCTCTTCGTA GATGTTACCT GGCACCCAGC TGGAGACCCT 
GGCTCAGACA AGGAGACCTC CTCCATGATG ATCGCCAGCA CAGCAGTAAA CTACTGCGGC 
TTGGAAACCA TCCTGCATAT GACCTGCTGC CAGCAGCGCC CGGAGGAGAT CACAGGCCAT 
CTGCACAGAG CCAAGCAGCT GGGCCTGAAG AACATAATGG CGCTGAGGGG AGACCCTGTA 
GGTGACCACT GGGAAGCAGA GGAAGGAGGC TTCAGCTATG CCACAGACCT GGTGAAGCAC 
ATCCGGACCG AGTTTGCTGA CTATTTTGAC ATCTGTGTGG CAGGTTACCC CAGAGGCCAC 
CCCGATGCAG AGAGCTTCGA GGATGACCTG AAGCATTTGA AGGAGAAGGT ATCTGCAGGC 
GCCGACTTCA TTATCACTCA GCTCTTCTTT GAGGCCAGCA CCTTCTTCAG CTTTGTGAAG 
GCCTGCACAG AGATAGGCAT CTCTTGCCCT ATCCTGCCTG GGATCTTCCC TATTCAGGGC 
TACACTTCCC TTCGGCAGCT TGTAAAACTG TCCAAGCTGG AGGTGCCACA GAAGATCAAG 
GATGTAATTG AGCCCATCAA AGACAACGAT GCTGCCATCC GCAACTACGG CATTGAGCTG 
GCTGTAAGCC TGTGCCGGGA GCTGCTGGAC AGTGGCTTGG TGCCAGGCCT CCACTTCTAT 
ACCCTCAACC GCGAGGTGGC CACCATGGAG GTGCTAAAGC AACTGGGCAT GTGGACCGAG 
GACCCCAGGC GTCCCTTGCC CTGGGCTCTC AGTGCGCATC CCAAGCGCCG GGAGGAAGAT 
GTCCGTCCCA TCTTCTGGGC CTCCAGACCA AAGAGCTACA TCTACCGCAC ACAGGACTGG 
GATGAGTTTC CTAACGGCCG CTGGGGTAAT TCTTCCTCAC CAGCCTTTGG GGAGCTGAAA 
GACTACTACC TCTTCTACCT GAAAAGCAAG TCCCCCAGGG AGGAGCTGCT GAAGATGTGG 
GGCGAGGAGC TCACCAGCGA AGAGAGTGTC TTTGAAGTCT TTGAACACTA CCTCTCTGGA 
GAGCCGAATC GCCATGGCTA CAGAGTGAGT GGGGTAACCT GCCTGCCCTG GAACGATGAA 
CCCCTGGCAG CGGAAACCAG CCTGATGAAG GAAGAGCTGC TCCGCGTGAA CAGGCTGGGC 
ATCCTCACCA TCAACTCTCA GCCCAACATC AACGCAAAAC CATCCTCAGA CCCTGTTGTG 
GGCTGGGGCC CCAGTGGGGG TTATGTCTTC CAGAAGGCCT ACCTCGAATT CTTCACCTCC 
CGTGAAACTG TGGAGGCGCT TCTGCAGGTG CTGAAGACAT ACGAGCTGCG GGTCAACTAC 
CACATCGTGG ACGTGAAGGG AGAGAACATC ACTAATGCCC CTGAGCTGCA GCCCAATGCC 
GTGACGTGGG GCATCTTCCC GGGTCGAGAG ATCATCCAGC CTACTGTGGT GGACCCCATC 
AGCTTCATGT TCTGGAAGGA TGAGGCCTTT GCCCTGTGGA TCGAGCAGTG GGGCAAGCTA 
TACGAGGAGG AGTCGCCATC CCGCATGATC ATCCAATACA TCCATGACAA CTATTTCCTG 
GTCAACCTGG TGGACAACGA GTTCCCGCTG GACAGCTGCC TGTGGCAGGT GGTGGAGGAC 
ACGTTTGAGC TGCTCAACAG GCATCCCACG GAGAGAGAGA CACAGGCTCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mthfr ( NM_001161798.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001155270.1 Mus musculus methylenetetrahydrofolate reductase (Mthfr), transcript variant 1, mRNA.

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CloneID	OMu18963
Clone ID Related Accession (Same CDS sequence)	NM_001161798.1
Accession Version	NM_001161798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	methylenetetrahydrofolate reductase isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK150159.1, BC100550.1 and BB726553.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052466.1, AK150159.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCATC AAAAAGCCAA GGTCCTCCCA GCTGGGCACT GTTATCCATC CCTGGGGATG 
TGGGCCTCAG AGGCTGGCTG CGTGAGGCTT TCTGTGCCAC CCTCTATCAG CAGGAATCCA 
GCCATGGTGA ACGAGGCCAG AGGAAGTGGC AGTCCCAACC CGCGATCTGA GGGCAGCAGC 
AGTGGCAGCG AGAGTTCCAA GGACAGTTCA AGATGTTCCA CCCCCAGCCT GGACCCAGAG 
CGGCACGAGA GACTCCGGGA GAAGATGAGG CGCAGAATGG ACTCTGGTGA CAAGTGGTTC 
TCCCTGGAGT TCTTCCCCCC TCGAACTGCT GAGGGAGCTG TTAACCTCAT CTCCAGGTTT 
GACCGGATGG CAGCAGGGGG CCCCCTCTTC GTAGATGTTA CCTGGCACCC AGCTGGAGAC 
CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACAGCAGT AAACTACTGC 
GGCTTGGAAA CCATCCTGCA TATGACCTGC TGCCAGCAGC GCCCGGAGGA GATCACAGGC 
CATCTGCACA GAGCCAAGCA GCTGGGCCTG AAGAACATAA TGGCGCTGAG GGGAGACCCT 
GTAGGTGACC ACTGGGAAGC AGAGGAAGGA GGCTTCAGCT ATGCCACAGA CCTGGTGAAG 
CACATCCGGA CCGAGTTTGC TGACTATTTT GACATCTGTG TGGCAGGTTA CCCCAGAGGC 
CACCCCGATG CAGAGAGCTT CGAGGATGAC CTGAAGCATT TGAAGGAGAA GGTATCTGCA 
GGCGCCGACT TCATTATCAC TCAGCTCTTC TTTGAGGCCA GCACCTTCTT CAGCTTTGTG 
AAGGCCTGCA CAGAGATAGG CATCTCTTGC CCTATCCTGC CTGGGATCTT CCCTATTCAG 
GGCTACACTT CCCTTCGGCA GCTTGTAAAA CTGTCCAAGC TGGAGGTGCC ACAGAAGATC 
AAGGATGTAA TTGAGCCCAT CAAAGACAAC GATGCTGCCA TCCGCAACTA CGGCATTGAG 
CTGGCTGTAA GCCTGTGCCG GGAGCTGCTG GACAGTGGCT TGGTGCCAGG CCTCCACTTC 
TATACCCTCA ACCGCGAGGT GGCCACCATG GAGGTGCTAA AGCAACTGGG CATGTGGACC 
GAGGACCCCA GGCGTCCCTT GCCCTGGGCT CTCAGTGCGC ATCCCAAGCG CCGGGAGGAA 
GATGTCCGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGCT ACATCTACCG CACACAGGAC 
TGGGATGAGT TTCCTAACGG CCGCTGGGGT AATTCTTCCT CACCAGCCTT TGGGGAGCTG 
AAAGACTACT ACCTCTTCTA CCTGAAAAGC AAGTCCCCCA GGGAGGAGCT GCTGAAGATG 
TGGGGCGAGG AGCTCACCAG CGAAGAGAGT GTCTTTGAAG TCTTTGAACA CTACCTCTCT 
GGAGAGCCGA ATCGCCATGG CTACAGAGTA ACCTGCCTGC CCTGGAACGA TGAACCCCTG 
GCAGCGGAAA CCAGCCTGAT GAAGGAAGAG CTGCTCCGCG TGAACAGGCT GGGCATCCTC 
ACCATCAACT CTCAGCCCAA CATCAACGCA AAACCATCCT CAGACCCTGT TGTGGGCTGG 
GGCCCCAGTG GGGGTTATGT CTTCCAGAAG GCCTACCTCG AATTCTTCAC CTCCCGTGAA 
ACTGTGGAGG CGCTTCTGCA GGTGCTGAAG ACATACGAGC TGCGGGTCAA CTACCACATC 
GTGGACGTGA AGGGAGAGAA CATCACTAAT GCCCCTGAGC TGCAGCCCAA TGCCGTGACG 
TGGGGCATCT TCCCGGGTCG AGAGATCATC CAGCCTACTG TGGTGGACCC CATCAGCTTC 
ATGTTCTGGA AGGATGAGGC CTTTGCCCTG TGGATCGAGC AGTGGGGCAA GCTATACGAG 
GAGGAGTCGC CATCCCGCAT GATCATCCAA TACATCCATG ACAACTATTT CCTGGTCAAC 
CTGGTGGACA ACGAGTTCCC GCTGGACAGC TGCCTGTGGC AGGTGGTGGA GGACACGTTT 
GAGCTGCTCA ACAGGCATCC CACGGAGAGA GAGACACAGG CTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155270.1
CDS	24..2111
Translation	MNHQKAKVLPAGHCYPSLGMWASEAGCVRLSVPPSISRNPAMVNEARGSGSPNPRSEGSSSGSESSKDSSRCSTPSLDPERHERLREKMRRRMDSGDKWFSLEFFPPRTAEGAVNLISRFDRMAAGGPLFVDVTWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCQQRPEEITGHLHRAKQLGLKNIMALRGDPVGDHWEAEEGGFSYATDLVKHIRTEFADYFDICVAGYPRGHPDAESFEDDLKHLKEKVSAGADFIITQLFFEASTFFSFVKACTEIGISCPILPGIFPIQGYTSLRQLVKLSKLEVPQKIKDVIEPIKDNDAAIRNYGIELAVSLCRELLDSGLVPGLHFYTLNREVATMEVLKQLGMWTEDPRRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQDWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFEHYLSGEPNRHGYRVTCLPWNDEPLAAETSLMKEELLRVNRLGILTINSQPNINAKPSSDPVVGWGPSGGYVFQKAYLEFFTSRETVEALLQVLKTYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPISFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDSCLWQVVEDTFELLNRHPTERETQAP

Target ORF information:

RefSeq Version	NM_001161798.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus methylenetetrahydrofolate reductase (Mthfr), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161798.1

ORF Insert Sequence:

ATGAACCATC AAAAAGCCAA GGTCCTCCCA GCTGGGCACT GTTATCCATC CCTGGGGATG 
TGGGCCTCAG AGGCTGGCTG CGTGAGGCTT TCTGTGCCAC CCTCTATCAG CAGGAATCCA 
GCCATGGTGA ACGAGGCCAG AGGAAGTGGC AGTCCCAACC CGCGATCTGA GGGCAGCAGC 
AGTGGCAGCG AGAGTTCCAA GGACAGTTCA AGATGTTCCA CCCCCAGCCT GGACCCAGAG 
CGGCACGAGA GACTCCGGGA GAAGATGAGG CGCAGAATGG ACTCTGGTGA CAAGTGGTTC 
TCCCTGGAGT TCTTCCCCCC TCGAACTGCT GAGGGAGCTG TTAACCTCAT CTCCAGGTTT 
GACCGGATGG CAGCAGGGGG CCCCCTCTTC GTAGATGTTA CCTGGCACCC AGCTGGAGAC 
CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACAGCAGT AAACTACTGC 
GGCTTGGAAA CCATCCTGCA TATGACCTGC TGCCAGCAGC GCCCGGAGGA GATCACAGGC 
CATCTGCACA GAGCCAAGCA GCTGGGCCTG AAGAACATAA TGGCGCTGAG GGGAGACCCT 
GTAGGTGACC ACTGGGAAGC AGAGGAAGGA GGCTTCAGCT ATGCCACAGA CCTGGTGAAG 
CACATCCGGA CCGAGTTTGC TGACTATTTT GACATCTGTG TGGCAGGTTA CCCCAGAGGC 
CACCCCGATG CAGAGAGCTT CGAGGATGAC CTGAAGCATT TGAAGGAGAA GGTATCTGCA 
GGCGCCGACT TCATTATCAC TCAGCTCTTC TTTGAGGCCA GCACCTTCTT CAGCTTTGTG 
AAGGCCTGCA CAGAGATAGG CATCTCTTGC CCTATCCTGC CTGGGATCTT CCCTATTCAG 
GGCTACACTT CCCTTCGGCA GCTTGTAAAA CTGTCCAAGC TGGAGGTGCC ACAGAAGATC 
AAGGATGTAA TTGAGCCCAT CAAAGACAAC GATGCTGCCA TCCGCAACTA CGGCATTGAG 
CTGGCTGTAA GCCTGTGCCG GGAGCTGCTG GACAGTGGCT TGGTGCCAGG CCTCCACTTC 
TATACCCTCA ACCGCGAGGT GGCCACCATG GAGGTGCTAA AGCAACTGGG CATGTGGACC 
GAGGACCCCA GGCGTCCCTT GCCCTGGGCT CTCAGTGCGC ATCCCAAGCG CCGGGAGGAA 
GATGTCCGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGCT ACATCTACCG CACACAGGAC 
TGGGATGAGT TTCCTAACGG CCGCTGGGGT AATTCTTCCT CACCAGCCTT TGGGGAGCTG 
AAAGACTACT ACCTCTTCTA CCTGAAAAGC AAGTCCCCCA GGGAGGAGCT GCTGAAGATG 
TGGGGCGAGG AGCTCACCAG CGAAGAGAGT GTCTTTGAAG TCTTTGAACA CTACCTCTCT 
GGAGAGCCGA ATCGCCATGG CTACAGAGTA ACCTGCCTGC CCTGGAACGA TGAACCCCTG 
GCAGCGGAAA CCAGCCTGAT GAAGGAAGAG CTGCTCCGCG TGAACAGGCT GGGCATCCTC 
ACCATCAACT CTCAGCCCAA CATCAACGCA AAACCATCCT CAGACCCTGT TGTGGGCTGG 
GGCCCCAGTG GGGGTTATGT CTTCCAGAAG GCCTACCTCG AATTCTTCAC CTCCCGTGAA 
ACTGTGGAGG CGCTTCTGCA GGTGCTGAAG ACATACGAGC TGCGGGTCAA CTACCACATC 
GTGGACGTGA AGGGAGAGAA CATCACTAAT GCCCCTGAGC TGCAGCCCAA TGCCGTGACG 
TGGGGCATCT TCCCGGGTCG AGAGATCATC CAGCCTACTG TGGTGGACCC CATCAGCTTC 
ATGTTCTGGA AGGATGAGGC CTTTGCCCTG TGGATCGAGC AGTGGGGCAA GCTATACGAG 
GAGGAGTCGC CATCCCGCAT GATCATCCAA TACATCCATG ACAACTATTT CCTGGTCAAC 
CTGGTGGACA ACGAGTTCCC GCTGGACAGC TGCCTGTGGC AGGTGGTGGA GGACACGTTT 
GAGCTGCTCA ACAGGCATCC CACGGAGAGA GAGACACAGG CTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mthfr ( XM_006538617.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538680.1 Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X1, mRNA.

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CloneID	OMu34036
Clone ID Related Accession (Same CDS sequence)	XM_006538617.3
Accession Version	XM_006538617.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	methylenetetrahydrofolate reductase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538617.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCATC AAAAAGCCAA GGTCCTCCCA GCTGGGCACT GTTATCCATC CCTGGGGATG 
TGGGCCTCAG AGGCTGGCTG CGTGAGGCTT TCTGTGCCAC CCTCTATCAG CAGGAATCCA 
GCCATGGTGA ACGAGGCCAG AGGAAGTGGC AGTCCCAACC CGCGATCTGA GGGCAGCAGC 
AGTGGCAGCG AGAGTTCCAA GGACAGTTCA AGATGTTCCA CCCCCAGCCT GGACCCAGAG 
CGGCACGAGA GACTCCGGGA GAAGATGAGG CGCAGAATGG ACTCTGGTGA CAAGTGGTTC 
TCCCTGGAGT TCTTCCCCCC TCGAACTGCT GAGGGAGCTG TTAACCTCAT CTCCAGGTTT 
GACCGGATGG CAGCAGGGGG CCCCCTCTTC GTAGATGTTA CCTGGCACCC AGCTGGAGAC 
CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACAGCAGT AAACTACTGC 
GGCTTGGAAA CCATCCTGCA TATGACCTGC TGCCAGCAGC GCCCGGAGGA GATCACAGGC 
CATCTGCACA GAGCCAAGCA GCTGGGCCTG AAGAACATAA TGGCGCTGAG GGGAGACCCT 
GTAGGTGACC ACTGGGAAGC AGAGGAAGGA GGCTTCAGCT ATGCCACAGA CCTGGTGAAG 
CACATCCGGA CCGAGTTTGC TGACTATTTT GACATCTGTG TGGCAGGTTA CCCCAGAGGC 
CACCCCGATG CAGAGAGCTT CGAGGATGAC CTGAAGCATT TGAAGGAGAA GGTATCTGCA 
GGCGCCGACT TCATTATCAC TCAGCTCTTC TTTGAGGCCA GCACCTTCTT CAGCTTTGTG 
AAGGCCTGCA CAGAGATAGG CATCTCTTGC CCTATCCTGC CTGGGATCTT CCCTATTCAG 
GGCTACACTT CCCTTCGGCA GCTTGTAAAA CTGTCCAAGC TGGAGGTGCC ACAGAAGATC 
AAGGATGTAA TTGAGCCCAT CAAAGACAAC GATGCTGCCA TCCGCAACTA CGGCATTGAG 
CTGGCTGTAA GCCTGTGCCG GGAGCTGCTG GACAGTGGCT TGGTGCCAGG CCTCCACTTC 
TATACCCTCA ACCGCGAGGT GGCCACCATG GAGGTGCTAA AGCAACTGGG CATGTGGACC 
GAGGACCCCA GGCGTCCCTT GCCCTGGGCT CTCAGTGCGC ATCCCAAGCG CCGGGAGGAA 
GATGTCCGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGCT ACATCTACCG CACACAGGAC 
TGGGATGAGT TTCCTAACGG CCGCTGGGGT AATTCTTCCT CACCAGCCTT TGGGGAGCTG 
AAAGACTACT ACCTCTTCTA CCTGAAAAGC AAGTCCCCCA GGGAGGAGCT GCTGAAGATG 
TGGGGCGAGG AGCTCACCAG CGAAGAGAGT GTCTTTGAAG TCTTTGAACA CTACCTCTCT 
GGAGAGCCGA ATCGCCATGG CTACAGAGTG AGTGGGGTAA CCTGCCTGCC CTGGAACGAT 
GAACCCCTGG CAGCGGAAAC CAGCCTGATG AAGGAAGAGC TGCTCCGCGT GAACAGGCTG 
GGCATCCTCA CCATCAACTC TCAGCCCAAC ATCAACGCAA AACCATCCTC AGACCCTGTT 
GTGGGCTGGG GCCCCAGTGG GGGTTATGTC TTCCAGAAGG CCTACCTCGA ATTCTTCACC 
TCCCGTGAAA CTGTGGAGGC GCTTCTGCAG GTGCTGAAGA CATACGAGCT GCGGGTCAAC 
TACCACATCG TGGACGTGAA GGGAGAGAAC ATCACTAATG CCCCTGAGCT GCAGCCCAAT 
GCCGTGACGT GGGGCATCTT CCCGGGTCGA GAGATCATCC AGCCTACTGT GGTGGACCCC 
ATCAGCTTCA TGTTCTGGAA GGATGAGGCC TTTGCCCTGT GGATCGAGCA GTGGGGCAAG 
CTATACGAGG AGGAGTCGCC ATCCCGCATG ATCATCCAAT ACATCCATGA CAACTATTTC 
CTGGTCAACC TGGTGGACAA CGAGTTCCCG CTGGACAGCT GCCTGTGGCA GGTGGTGGAG 
GACACGTTTG AGCTGCTCAA CAGGCATCCC ACGGAGAGAG AGACACAGGC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538680.1
CDS	713..2809
Translation	MNHQKAKVLPAGHCYPSLGMWASEAGCVRLSVPPSISRNPAMVNEARGSGSPNPRSEGSSSGSESSKDSSRCSTPSLDPERHERLREKMRRRMDSGDKWFSLEFFPPRTAEGAVNLISRFDRMAAGGPLFVDVTWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCQQRPEEITGHLHRAKQLGLKNIMALRGDPVGDHWEAEEGGFSYATDLVKHIRTEFADYFDICVAGYPRGHPDAESFEDDLKHLKEKVSAGADFIITQLFFEASTFFSFVKACTEIGISCPILPGIFPIQGYTSLRQLVKLSKLEVPQKIKDVIEPIKDNDAAIRNYGIELAVSLCRELLDSGLVPGLHFYTLNREVATMEVLKQLGMWTEDPRRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQDWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFEHYLSGEPNRHGYRVSGVTCLPWNDEPLAAETSLMKEELLRVNRLGILTINSQPNINAKPSSDPVVGWGPSGGYVFQKAYLEFFTSRETVEALLQVLKTYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPISFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDSCLWQVVEDTFELLNRHPTERETQAP

Target ORF information:

RefSeq Version	XM_006538617.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538617.3

ORF Insert Sequence:

ATGAACCATC AAAAAGCCAA GGTCCTCCCA GCTGGGCACT GTTATCCATC CCTGGGGATG 
TGGGCCTCAG AGGCTGGCTG CGTGAGGCTT TCTGTGCCAC CCTCTATCAG CAGGAATCCA 
GCCATGGTGA ACGAGGCCAG AGGAAGTGGC AGTCCCAACC CGCGATCTGA GGGCAGCAGC 
AGTGGCAGCG AGAGTTCCAA GGACAGTTCA AGATGTTCCA CCCCCAGCCT GGACCCAGAG 
CGGCACGAGA GACTCCGGGA GAAGATGAGG CGCAGAATGG ACTCTGGTGA CAAGTGGTTC 
TCCCTGGAGT TCTTCCCCCC TCGAACTGCT GAGGGAGCTG TTAACCTCAT CTCCAGGTTT 
GACCGGATGG CAGCAGGGGG CCCCCTCTTC GTAGATGTTA CCTGGCACCC AGCTGGAGAC 
CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACAGCAGT AAACTACTGC 
GGCTTGGAAA CCATCCTGCA TATGACCTGC TGCCAGCAGC GCCCGGAGGA GATCACAGGC 
CATCTGCACA GAGCCAAGCA GCTGGGCCTG AAGAACATAA TGGCGCTGAG GGGAGACCCT 
GTAGGTGACC ACTGGGAAGC AGAGGAAGGA GGCTTCAGCT ATGCCACAGA CCTGGTGAAG 
CACATCCGGA CCGAGTTTGC TGACTATTTT GACATCTGTG TGGCAGGTTA CCCCAGAGGC 
CACCCCGATG CAGAGAGCTT CGAGGATGAC CTGAAGCATT TGAAGGAGAA GGTATCTGCA 
GGCGCCGACT TCATTATCAC TCAGCTCTTC TTTGAGGCCA GCACCTTCTT CAGCTTTGTG 
AAGGCCTGCA CAGAGATAGG CATCTCTTGC CCTATCCTGC CTGGGATCTT CCCTATTCAG 
GGCTACACTT CCCTTCGGCA GCTTGTAAAA CTGTCCAAGC TGGAGGTGCC ACAGAAGATC 
AAGGATGTAA TTGAGCCCAT CAAAGACAAC GATGCTGCCA TCCGCAACTA CGGCATTGAG 
CTGGCTGTAA GCCTGTGCCG GGAGCTGCTG GACAGTGGCT TGGTGCCAGG CCTCCACTTC 
TATACCCTCA ACCGCGAGGT GGCCACCATG GAGGTGCTAA AGCAACTGGG CATGTGGACC 
GAGGACCCCA GGCGTCCCTT GCCCTGGGCT CTCAGTGCGC ATCCCAAGCG CCGGGAGGAA 
GATGTCCGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGCT ACATCTACCG CACACAGGAC 
TGGGATGAGT TTCCTAACGG CCGCTGGGGT AATTCTTCCT CACCAGCCTT TGGGGAGCTG 
AAAGACTACT ACCTCTTCTA CCTGAAAAGC AAGTCCCCCA GGGAGGAGCT GCTGAAGATG 
TGGGGCGAGG AGCTCACCAG CGAAGAGAGT GTCTTTGAAG TCTTTGAACA CTACCTCTCT 
GGAGAGCCGA ATCGCCATGG CTACAGAGTG AGTGGGGTAA CCTGCCTGCC CTGGAACGAT 
GAACCCCTGG CAGCGGAAAC CAGCCTGATG AAGGAAGAGC TGCTCCGCGT GAACAGGCTG 
GGCATCCTCA CCATCAACTC TCAGCCCAAC ATCAACGCAA AACCATCCTC AGACCCTGTT 
GTGGGCTGGG GCCCCAGTGG GGGTTATGTC TTCCAGAAGG CCTACCTCGA ATTCTTCACC 
TCCCGTGAAA CTGTGGAGGC GCTTCTGCAG GTGCTGAAGA CATACGAGCT GCGGGTCAAC 
TACCACATCG TGGACGTGAA GGGAGAGAAC ATCACTAATG CCCCTGAGCT GCAGCCCAAT 
GCCGTGACGT GGGGCATCTT CCCGGGTCGA GAGATCATCC AGCCTACTGT GGTGGACCCC 
ATCAGCTTCA TGTTCTGGAA GGATGAGGCC TTTGCCCTGT GGATCGAGCA GTGGGGCAAG 
CTATACGAGG AGGAGTCGCC ATCCCGCATG ATCATCCAAT ACATCCATGA CAACTATTTC 
CTGGTCAACC TGGTGGACAA CGAGTTCCCG CTGGACAGCT GCCTGTGGCA GGTGGTGGAG 
GACACGTTTG AGCTGCTCAA CAGGCATCCC ACGGAGAGAG AGACACAGGC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mthfr ( XM_006538618.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538681.1 Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu34037
Clone ID Related Accession (Same CDS sequence)	XM_006538618.3
Accession Version	XM_006538618.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	methylenetetrahydrofolate reductase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538618.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGCA TTAATTCAAG CTGGCCGAGG CCCTCCCTCG GAGTCCAGAG AGAGGTTATA 
GAACAGAACT CTGGCTGGGG CACGCGGAGC AGGAATCCAG CCATGGTGAA CGAGGCCAGA 
GGAAGTGGCA GTCCCAACCC GCGATCTGAG GGCAGCAGCA GTGGCAGCGA GAGTTCCAAG 
GACAGTTCAA GATGTTCCAC CCCCAGCCTG GACCCAGAGC GGCACGAGAG ACTCCGGGAG 
AAGATGAGGC GCAGAATGGA CTCTGGTGAC AAGTGGTTCT CCCTGGAGTT CTTCCCCCCT 
CGAACTGCTG AGGGAGCTGT TAACCTCATC TCCAGGTTTG ACCGGATGGC AGCAGGGGGC 
CCCCTCTTCG TAGATGTTAC CTGGCACCCA GCTGGAGACC CTGGCTCAGA CAAGGAGACC 
TCCTCCATGA TGATCGCCAG CACAGCAGTA AACTACTGCG GCTTGGAAAC CATCCTGCAT 
ATGACCTGCT GCCAGCAGCG CCCGGAGGAG ATCACAGGCC ATCTGCACAG AGCCAAGCAG 
CTGGGCCTGA AGAACATAAT GGCGCTGAGG GGAGACCCTG TAGGTGACCA CTGGGAAGCA 
GAGGAAGGAG GCTTCAGCTA TGCCACAGAC CTGGTGAAGC ACATCCGGAC CGAGTTTGCT 
GACTATTTTG ACATCTGTGT GGCAGGTTAC CCCAGAGGCC ACCCCGATGC AGAGAGCTTC 
GAGGATGACC TGAAGCATTT GAAGGAGAAG GTATCTGCAG GCGCCGACTT CATTATCACT 
CAGCTCTTCT TTGAGGCCAG CACCTTCTTC AGCTTTGTGA AGGCCTGCAC AGAGATAGGC 
ATCTCTTGCC CTATCCTGCC TGGGATCTTC CCTATTCAGG GCTACACTTC CCTTCGGCAG 
CTTGTAAAAC TGTCCAAGCT GGAGGTGCCA CAGAAGATCA AGGATGTAAT TGAGCCCATC 
AAAGACAACG ATGCTGCCAT CCGCAACTAC GGCATTGAGC TGGCTGTAAG CCTGTGCCGG 
GAGCTGCTGG ACAGTGGCTT GGTGCCAGGC CTCCACTTCT ATACCCTCAA CCGCGAGGTG 
GCCACCATGG AGGTGCTAAA GCAACTGGGC ATGTGGACCG AGGACCCCAG GCGTCCCTTG 
CCCTGGGCTC TCAGTGCGCA TCCCAAGCGC CGGGAGGAAG ATGTCCGTCC CATCTTCTGG 
GCCTCCAGAC CAAAGAGCTA CATCTACCGC ACACAGGACT GGGATGAGTT TCCTAACGGC 
CGCTGGGGTA ATTCTTCCTC ACCAGCCTTT GGGGAGCTGA AAGACTACTA CCTCTTCTAC 
CTGAAAAGCA AGTCCCCCAG GGAGGAGCTG CTGAAGATGT GGGGCGAGGA GCTCACCAGC 
GAAGAGAGTG TCTTTGAAGT CTTTGAACAC TACCTCTCTG GAGAGCCGAA TCGCCATGGC 
TACAGAGTGA GTGGGGTAAC CTGCCTGCCC TGGAACGATG AACCCCTGGC AGCGGAAACC 
AGCCTGATGA AGGAAGAGCT GCTCCGCGTG AACAGGCTGG GCATCCTCAC CATCAACTCT 
CAGCCCAACA TCAACGCAAA ACCATCCTCA GACCCTGTTG TGGGCTGGGG CCCCAGTGGG 
GGTTATGTCT TCCAGAAGGC CTACCTCGAA TTCTTCACCT CCCGTGAAAC TGTGGAGGCG 
CTTCTGCAGG TGCTGAAGAC ATACGAGCTG CGGGTCAACT ACCACATCGT GGACGTGAAG 
GGAGAGAACA TCACTAATGC CCCTGAGCTG CAGCCCAATG CCGTGACGTG GGGCATCTTC 
CCGGGTCGAG AGATCATCCA GCCTACTGTG GTGGACCCCA TCAGCTTCAT GTTCTGGAAG 
GATGAGGCCT TTGCCCTGTG GATCGAGCAG TGGGGCAAGC TATACGAGGA GGAGTCGCCA 
TCCCGCATGA TCATCCAATA CATCCATGAC AACTATTTCC TGGTCAACCT GGTGGACAAC 
GAGTTCCCGC TGGACAGCTG CCTGTGGCAG GTGGTGGAGG ACACGTTTGA GCTGCTCAAC 
AGGCATCCCA CGGAGAGAGA GACACAGGCT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538681.1
CDS	120..2195
Translation	MDGINSSWPRPSLGVQREVIEQNSGWGTRSRNPAMVNEARGSGSPNPRSEGSSSGSESSKDSSRCSTPSLDPERHERLREKMRRRMDSGDKWFSLEFFPPRTAEGAVNLISRFDRMAAGGPLFVDVTWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCQQRPEEITGHLHRAKQLGLKNIMALRGDPVGDHWEAEEGGFSYATDLVKHIRTEFADYFDICVAGYPRGHPDAESFEDDLKHLKEKVSAGADFIITQLFFEASTFFSFVKACTEIGISCPILPGIFPIQGYTSLRQLVKLSKLEVPQKIKDVIEPIKDNDAAIRNYGIELAVSLCRELLDSGLVPGLHFYTLNREVATMEVLKQLGMWTEDPRRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQDWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFEHYLSGEPNRHGYRVSGVTCLPWNDEPLAAETSLMKEELLRVNRLGILTINSQPNINAKPSSDPVVGWGPSGGYVFQKAYLEFFTSRETVEALLQVLKTYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPISFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDSCLWQVVEDTFELLNRHPTERETQAP

Target ORF information:

RefSeq Version	XM_006538618.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus 5,10-methylenetetrahydrofolate reductase (Mthfr), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538618.3

ORF Insert Sequence:

ATGGACGGCA TTAATTCAAG CTGGCCGAGG CCCTCCCTCG GAGTCCAGAG AGAGGTTATA 
GAACAGAACT CTGGCTGGGG CACGCGGAGC AGGAATCCAG CCATGGTGAA CGAGGCCAGA 
GGAAGTGGCA GTCCCAACCC GCGATCTGAG GGCAGCAGCA GTGGCAGCGA GAGTTCCAAG 
GACAGTTCAA GATGTTCCAC CCCCAGCCTG GACCCAGAGC GGCACGAGAG ACTCCGGGAG 
AAGATGAGGC GCAGAATGGA CTCTGGTGAC AAGTGGTTCT CCCTGGAGTT CTTCCCCCCT 
CGAACTGCTG AGGGAGCTGT TAACCTCATC TCCAGGTTTG ACCGGATGGC AGCAGGGGGC 
CCCCTCTTCG TAGATGTTAC CTGGCACCCA GCTGGAGACC CTGGCTCAGA CAAGGAGACC 
TCCTCCATGA TGATCGCCAG CACAGCAGTA AACTACTGCG GCTTGGAAAC CATCCTGCAT 
ATGACCTGCT GCCAGCAGCG CCCGGAGGAG ATCACAGGCC ATCTGCACAG AGCCAAGCAG 
CTGGGCCTGA AGAACATAAT GGCGCTGAGG GGAGACCCTG TAGGTGACCA CTGGGAAGCA 
GAGGAAGGAG GCTTCAGCTA TGCCACAGAC CTGGTGAAGC ACATCCGGAC CGAGTTTGCT 
GACTATTTTG ACATCTGTGT GGCAGGTTAC CCCAGAGGCC ACCCCGATGC AGAGAGCTTC 
GAGGATGACC TGAAGCATTT GAAGGAGAAG GTATCTGCAG GCGCCGACTT CATTATCACT 
CAGCTCTTCT TTGAGGCCAG CACCTTCTTC AGCTTTGTGA AGGCCTGCAC AGAGATAGGC 
ATCTCTTGCC CTATCCTGCC TGGGATCTTC CCTATTCAGG GCTACACTTC CCTTCGGCAG 
CTTGTAAAAC TGTCCAAGCT GGAGGTGCCA CAGAAGATCA AGGATGTAAT TGAGCCCATC 
AAAGACAACG ATGCTGCCAT CCGCAACTAC GGCATTGAGC TGGCTGTAAG CCTGTGCCGG 
GAGCTGCTGG ACAGTGGCTT GGTGCCAGGC CTCCACTTCT ATACCCTCAA CCGCGAGGTG 
GCCACCATGG AGGTGCTAAA GCAACTGGGC ATGTGGACCG AGGACCCCAG GCGTCCCTTG 
CCCTGGGCTC TCAGTGCGCA TCCCAAGCGC CGGGAGGAAG ATGTCCGTCC CATCTTCTGG 
GCCTCCAGAC CAAAGAGCTA CATCTACCGC ACACAGGACT GGGATGAGTT TCCTAACGGC 
CGCTGGGGTA ATTCTTCCTC ACCAGCCTTT GGGGAGCTGA AAGACTACTA CCTCTTCTAC 
CTGAAAAGCA AGTCCCCCAG GGAGGAGCTG CTGAAGATGT GGGGCGAGGA GCTCACCAGC 
GAAGAGAGTG TCTTTGAAGT CTTTGAACAC TACCTCTCTG GAGAGCCGAA TCGCCATGGC 
TACAGAGTGA GTGGGGTAAC CTGCCTGCCC TGGAACGATG AACCCCTGGC AGCGGAAACC 
AGCCTGATGA AGGAAGAGCT GCTCCGCGTG AACAGGCTGG GCATCCTCAC CATCAACTCT 
CAGCCCAACA TCAACGCAAA ACCATCCTCA GACCCTGTTG TGGGCTGGGG CCCCAGTGGG 
GGTTATGTCT TCCAGAAGGC CTACCTCGAA TTCTTCACCT CCCGTGAAAC TGTGGAGGCG 
CTTCTGCAGG TGCTGAAGAC ATACGAGCTG CGGGTCAACT ACCACATCGT GGACGTGAAG 
GGAGAGAACA TCACTAATGC CCCTGAGCTG CAGCCCAATG CCGTGACGTG GGGCATCTTC 
CCGGGTCGAG AGATCATCCA GCCTACTGTG GTGGACCCCA TCAGCTTCAT GTTCTGGAAG 
GATGAGGCCT TTGCCCTGTG GATCGAGCAG TGGGGCAAGC TATACGAGGA GGAGTCGCCA 
TCCCGCATGA TCATCCAATA CATCCATGAC AACTATTTCC TGGTCAACCT GGTGGACAAC 
GAGTTCCCGC TGGACAGCTG CCTGTGGCAG GTGGTGGAGG ACACGTTTGA GCTGCTCAAC 
AGGCATCCCA CGGAGAGAGA GACACAGGCT CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mthfr ( NM_010840.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034970.2 Mus musculus methylenetetrahydrofolate reductase (Mthfr), transcript variant 2, mRNA.

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CloneID	OMu19413
Clone ID Related Accession (Same CDS sequence)	NM_010840.3
Accession Version	NM_010840.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	methylenetetrahydrofolate reductase isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL606929.13, AK030192.1, BC100550.1 and BB726553.1. On Jun 17, 2009 this sequence version replaced NM_010840.2. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream translational start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK030192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGTGAACG AGGCCAGAGG AAGTGGCAGT CCCAACCCGC GATCTGAGGG CAGCAGCAGT 
GGCAGCGAGA GTTCCAAGGA CAGTTCAAGA TGTTCCACCC CCAGCCTGGA CCCAGAGCGG 
CACGAGAGAC TCCGGGAGAA GATGAGGCGC AGAATGGACT CTGGTGACAA GTGGTTCTCC 
CTGGAGTTCT TCCCCCCTCG AACTGCTGAG GGAGCTGTTA ACCTCATCTC CAGGTTTGAC 
CGGATGGCAG CAGGGGGCCC CCTCTTCGTA GATGTTACCT GGCACCCAGC TGGAGACCCT 
GGCTCAGACA AGGAGACCTC CTCCATGATG ATCGCCAGCA CAGCAGTAAA CTACTGCGGC 
TTGGAAACCA TCCTGCATAT GACCTGCTGC CAGCAGCGCC CGGAGGAGAT CACAGGCCAT 
CTGCACAGAG CCAAGCAGCT GGGCCTGAAG AACATAATGG CGCTGAGGGG AGACCCTGTA 
GGTGACCACT GGGAAGCAGA GGAAGGAGGC TTCAGCTATG CCACAGACCT GGTGAAGCAC 
ATCCGGACCG AGTTTGCTGA CTATTTTGAC ATCTGTGTGG CAGGTTACCC CAGAGGCCAC 
CCCGATGCAG AGAGCTTCGA GGATGACCTG AAGCATTTGA AGGAGAAGGT ATCTGCAGGC 
GCCGACTTCA TTATCACTCA GCTCTTCTTT GAGGCCAGCA CCTTCTTCAG CTTTGTGAAG 
GCCTGCACAG AGATAGGCAT CTCTTGCCCT ATCCTGCCTG GGATCTTCCC TATTCAGGGC 
TACACTTCCC TTCGGCAGCT TGTAAAACTG TCCAAGCTGG AGGTGCCACA GAAGATCAAG 
GATGTAATTG AGCCCATCAA AGACAACGAT GCTGCCATCC GCAACTACGG CATTGAGCTG 
GCTGTAAGCC TGTGCCGGGA GCTGCTGGAC AGTGGCTTGG TGCCAGGCCT CCACTTCTAT 
ACCCTCAACC GCGAGGTGGC CACCATGGAG GTGCTAAAGC AACTGGGCAT GTGGACCGAG 
GACCCCAGGC GTCCCTTGCC CTGGGCTCTC AGTGCGCATC CCAAGCGCCG GGAGGAAGAT 
GTCCGTCCCA TCTTCTGGGC CTCCAGACCA AAGAGCTACA TCTACCGCAC ACAGGACTGG 
GATGAGTTTC CTAACGGCCG CTGGGGTAAT TCTTCCTCAC CAGCCTTTGG GGAGCTGAAA 
GACTACTACC TCTTCTACCT GAAAAGCAAG TCCCCCAGGG AGGAGCTGCT GAAGATGTGG 
GGCGAGGAGC TCACCAGCGA AGAGAGTGTC TTTGAAGTCT TTGAACACTA CCTCTCTGGA 
GAGCCGAATC GCCATGGCTA CAGAGTAACC TGCCTGCCCT GGAACGATGA ACCCCTGGCA 
GCGGAAACCA GCCTGATGAA GGAAGAGCTG CTCCGCGTGA ACAGGCTGGG CATCCTCACC 
ATCAACTCTC AGCCCAACAT CAACGCAAAA CCATCCTCAG ACCCTGTTGT GGGCTGGGGC 
CCCAGTGGGG GTTATGTCTT CCAGAAGGCC TACCTCGAAT TCTTCACCTC CCGTGAAACT 
GTGGAGGCGC TTCTGCAGGT GCTGAAGACA TACGAGCTGC GGGTCAACTA CCACATCGTG 
GACGTGAAGG GAGAGAACAT CACTAATGCC CCTGAGCTGC AGCCCAATGC CGTGACGTGG 
GGCATCTTCC CGGGTCGAGA GATCATCCAG CCTACTGTGG TGGACCCCAT CAGCTTCATG 
TTCTGGAAGG ATGAGGCCTT TGCCCTGTGG ATCGAGCAGT GGGGCAAGCT ATACGAGGAG 
GAGTCGCCAT CCCGCATGAT CATCCAATAC ATCCATGACA ACTATTTCCT GGTCAACCTG 
GTGGACAACG AGTTCCCGCT GGACAGCTGC CTGTGGCAGG TGGTGGAGGA CACGTTTGAG 
CTGCTCAACA GGCATCCCAC GGAGAGAGAG ACACAGGCTC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034970.2
CDS	70..2034
Translation	MVNEARGSGSPNPRSEGSSSGSESSKDSSRCSTPSLDPERHERLREKMRRRMDSGDKWFSLEFFPPRTAEGAVNLISRFDRMAAGGPLFVDVTWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCQQRPEEITGHLHRAKQLGLKNIMALRGDPVGDHWEAEEGGFSYATDLVKHIRTEFADYFDICVAGYPRGHPDAESFEDDLKHLKEKVSAGADFIITQLFFEASTFFSFVKACTEIGISCPILPGIFPIQGYTSLRQLVKLSKLEVPQKIKDVIEPIKDNDAAIRNYGIELAVSLCRELLDSGLVPGLHFYTLNREVATMEVLKQLGMWTEDPRRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQDWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPREELLKMWGEELTSEESVFEVFEHYLSGEPNRHGYRVTCLPWNDEPLAAETSLMKEELLRVNRLGILTINSQPNINAKPSSDPVVGWGPSGGYVFQKAYLEFFTSRETVEALLQVLKTYELRVNYHIVDVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPISFMFWKDEAFALWIEQWGKLYEEESPSRMIIQYIHDNYFLVNLVDNEFPLDSCLWQVVEDTFELLNRHPTERETQAP

Target ORF information:

RefSeq Version	NM_010840.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus methylenetetrahydrofolate reductase (Mthfr), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010840.3

ORF Insert Sequence:

ATGGTGAACG AGGCCAGAGG AAGTGGCAGT CCCAACCCGC GATCTGAGGG CAGCAGCAGT 
GGCAGCGAGA GTTCCAAGGA CAGTTCAAGA TGTTCCACCC CCAGCCTGGA CCCAGAGCGG 
CACGAGAGAC TCCGGGAGAA GATGAGGCGC AGAATGGACT CTGGTGACAA GTGGTTCTCC 
CTGGAGTTCT TCCCCCCTCG AACTGCTGAG GGAGCTGTTA ACCTCATCTC CAGGTTTGAC 
CGGATGGCAG CAGGGGGCCC CCTCTTCGTA GATGTTACCT GGCACCCAGC TGGAGACCCT 
GGCTCAGACA AGGAGACCTC CTCCATGATG ATCGCCAGCA CAGCAGTAAA CTACTGCGGC 
TTGGAAACCA TCCTGCATAT GACCTGCTGC CAGCAGCGCC CGGAGGAGAT CACAGGCCAT 
CTGCACAGAG CCAAGCAGCT GGGCCTGAAG AACATAATGG CGCTGAGGGG AGACCCTGTA 
GGTGACCACT GGGAAGCAGA GGAAGGAGGC TTCAGCTATG CCACAGACCT GGTGAAGCAC 
ATCCGGACCG AGTTTGCTGA CTATTTTGAC ATCTGTGTGG CAGGTTACCC CAGAGGCCAC 
CCCGATGCAG AGAGCTTCGA GGATGACCTG AAGCATTTGA AGGAGAAGGT ATCTGCAGGC 
GCCGACTTCA TTATCACTCA GCTCTTCTTT GAGGCCAGCA CCTTCTTCAG CTTTGTGAAG 
GCCTGCACAG AGATAGGCAT CTCTTGCCCT ATCCTGCCTG GGATCTTCCC TATTCAGGGC 
TACACTTCCC TTCGGCAGCT TGTAAAACTG TCCAAGCTGG AGGTGCCACA GAAGATCAAG 
GATGTAATTG AGCCCATCAA AGACAACGAT GCTGCCATCC GCAACTACGG CATTGAGCTG 
GCTGTAAGCC TGTGCCGGGA GCTGCTGGAC AGTGGCTTGG TGCCAGGCCT CCACTTCTAT 
ACCCTCAACC GCGAGGTGGC CACCATGGAG GTGCTAAAGC AACTGGGCAT GTGGACCGAG 
GACCCCAGGC GTCCCTTGCC CTGGGCTCTC AGTGCGCATC CCAAGCGCCG GGAGGAAGAT 
GTCCGTCCCA TCTTCTGGGC CTCCAGACCA AAGAGCTACA TCTACCGCAC ACAGGACTGG 
GATGAGTTTC CTAACGGCCG CTGGGGTAAT TCTTCCTCAC CAGCCTTTGG GGAGCTGAAA 
GACTACTACC TCTTCTACCT GAAAAGCAAG TCCCCCAGGG AGGAGCTGCT GAAGATGTGG 
GGCGAGGAGC TCACCAGCGA AGAGAGTGTC TTTGAAGTCT TTGAACACTA CCTCTCTGGA 
GAGCCGAATC GCCATGGCTA CAGAGTAACC TGCCTGCCCT GGAACGATGA ACCCCTGGCA 
GCGGAAACCA GCCTGATGAA GGAAGAGCTG CTCCGCGTGA ACAGGCTGGG CATCCTCACC 
ATCAACTCTC AGCCCAACAT CAACGCAAAA CCATCCTCAG ACCCTGTTGT GGGCTGGGGC 
CCCAGTGGGG GTTATGTCTT CCAGAAGGCC TACCTCGAAT TCTTCACCTC CCGTGAAACT 
GTGGAGGCGC TTCTGCAGGT GCTGAAGACA TACGAGCTGC GGGTCAACTA CCACATCGTG 
GACGTGAAGG GAGAGAACAT CACTAATGCC CCTGAGCTGC AGCCCAATGC CGTGACGTGG 
GGCATCTTCC CGGGTCGAGA GATCATCCAG CCTACTGTGG TGGACCCCAT CAGCTTCATG 
TTCTGGAAGG ATGAGGCCTT TGCCCTGTGG ATCGAGCAGT GGGGCAAGCT ATACGAGGAG 
GAGTCGCCAT CCCGCATGAT CATCCAATAC ATCCATGACA ACTATTTCCT GGTCAACCTG 
GTGGACAACG AGTTCCCGCT GGACAGCTGC CTGTGGCAGG TGGTGGAGGA CACGTTTGAG 
CTGCTCAACA GGCATCCCAC GGAGAGAGAG ACACAGGCTC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Methylene-tetrahydrofolate reductase contributes to allergic airway disease.
PloS one13(1)e0190916(2018)
Eyring KR,Pedersen BS,Maclean KN,Stabler SP,Yang IV,Schwartz DA

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Mthfr cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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