agrn cDNA ORF clone, Danio rerio(zebrafish)

The following agrn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the agrn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa151480 XM_021469834.1
Latest version!
Danio rerio agrin (agrn), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa151481 XM_021469835.1
Latest version!
Danio rerio agrin (agrn), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa151482 XM_021469836.1
Latest version!
Danio rerio agrin (agrn), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa44579 XM_009296865.2
Latest version!
Danio rerio agrin (agrn), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa44580 XM_009296866.2
Latest version!
Danio rerio agrin (agrn), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa44581 XM_009296867.3
Latest version!
Danio rerio agrin (agrn), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa151475 XM_021469829.1
Latest version!
Danio rerio agrin (agrn), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa151476 XM_021469830.1
Latest version!
Danio rerio agrin (agrn), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa151477 XM_021469831.1
Latest version!
Danio rerio agrin (agrn), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa151478 XM_021469832.1
Latest version!
Danio rerio agrin (agrn), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa151479 XM_021469833.1
Latest version!
Danio rerio agrin (agrn), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa151483 XM_021469837.1
Latest version!
Danio rerio agrin (agrn), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa57387 XM_017353587.1
Latest version!
Danio rerio agrin (agrn), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa33967 NM_001177452.1
Latest version!
Danio rerio agrin (agrn), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa44574 XM_009296860.3
Latest version!
Danio rerio agrin (agrn), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa44575 XM_009296861.2
Latest version!
Danio rerio agrin (agrn), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa44576 XM_009296862.2
Latest version!
Danio rerio agrin (agrn), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa44577 XM_009296863.3
Latest version!
Danio rerio agrin (agrn), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa44578 XM_009296864.3
Latest version!
Danio rerio agrin (agrn), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
ODa44581 XM_009296867.2 Danio rerio agrin (agrn), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa44574 XM_009296860.2 Danio rerio agrin (agrn), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa44578 XM_009296864.2 Danio rerio agrin (agrn), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa44577 XM_009296863.2 Danio rerio agrin (agrn), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa151480
    Clone ID Related Accession (Same CDS sequence) XM_021469834.1
    Accession Version XM_021469834.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product agrin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGACCAGTT GTCAGTACCC CACGCCGCCC CCAGAGCGGG ACCGCATGTA CCAGCACAAA 
    GTGTCGCTGG TCGTGCGATA CTTCATGATT CCTTGCAACA TCTGTCTCAT ACTGCTGGCC
    ACATCCACGC TCGGCTTTGC TGTACTCCTC TTCCTCAATA ACTGTAGGTC ACAGTTAGTG
    AAACATGACA TGCAGCATAA ACCTGTTCAC TTCACACCGG CACCTCCTCC TGATGGTTGC
    AGAGGGAAGT TGTGCGGATT TGGAGCGGTG TGTGAGAGAG ACCAAGCTGA CCCATCTAAA
    GGAGAGTGTG TGTGTAAGAA GATTGTGTGT ACATCTGTGG TGGCGCCAGT GTGCGGATCT
    GACTCCTCCA CCTATTCCAA TGAGTGTGAG CTGGAGAAGG CTCAGTGCAA TACACAGAGA
    CGCATCAAGG TGATGCGCAA AGGCCCCTGC GCCCTCAAGG ACCCGTGCAG TGAGGTAACC
    TGTAGCTTTG GCAGCACCTG CATCCAGTCA TCGGATGGTC TGTCCGCCAA GTGTATGTGT
    CCTCTGAGCT GTGAAAACGT CCCCAAGCAT GTGGTGTGTG GCAGTGACGG CCTGGACTAC
    CAAAGCGAAT GTGAACTCAA CATGAAGGCC TGCGCCACTC AGAAGAACAT CCGTGTGCAT
    CACCAGGGCC GCTGCGATCC TTGCAGTGAC ACTCTGAACA GCTTAAGCAC AGCCTGTCGA
    GTCAACCCAA AGACCTACCA GCAGATTACC TTTGCCCCGG CTGAGTCTTG TCCGCCTGAC
    GAAATGCCCA TCTGTGCCAG CGACGGACAC ACCTACGAGA GCATGTGTCA GATGGAGCGC
    ACTGCCCTGA AGAACAACCT GGAGCTCAAA AAGGTCTCTT TAGGCAGCTG CAAGGAAAAA
    GGGGGCTGCC CGAATGGTTG TAAGTTCAAC GCCATATGTT TGCTGGATAA TGGAGAGTTT
    CGGTGCTCCT GCGATCCCAT ACAATGCGAC GGCACGTACA AGCCCCTGTG CGGAAAGGAT
    GGCAAGACAT ACCCCAACGA CTGCGAGAGG AAACTGCAGG AATGCCGGAC CCAAAAAGAC
    ATCCCTGTCA AACAACAAGG GCCCTGTGAT CTTAACCTGG AGAGCCCCTG CCTGAAGAAG
    ACCTGCGAGT TTGGAGCCGT GTGCGTGGTG AAGAACAGCG AAGCAGTGTG TGAATGCTCA
    GACGCCTGTC CGCAGGACCA AGACCCTGTT TGCGGCAGCG ATGGGCACAC GTACAGCAGC
    TCATGTCAAA TGAAGGCCAT GGGATGCGCT CTTCAGAAAC AGATACAGAT GCAACACAAA
    GGACCATGTG CAGATGAGTC CTGCACCAAC TGCTCGTTTG GCGCGATCTG TGATGCCCAG
    ACTGGCCGGT GCGTGTGCCC TAAAGGCTGT TTGGAAACAC GGCAGCCCGT ATGCGGCAGC
    GACGGCACCA CGTATGACAA CGAGTGCAAG CTCAACGTCC AAGCCTGCAC TAAACAGCTG
    GATCTCAAGG TGGTGGCCCA TGGAGAATGC AAAACCTGTG GCAGCACTGT GTGTAGCTGG
    GGGTCGCAGT GCGTGAACAA CAAGTGTGAG TGTCAGCAGT GCACTGGTCA GCCGGTGAAA
    CCCGTGTGTG GCAGCGACGG CAACACATAC AACAACGACT GCGAGCTGAG ACTGGCCTCC
    TGTCAGAAAC AGAGGAAGAT AGAGGTGGCC AAGCCTGGCG TCTGCGATGA AGACTGTGGT
    TCAGGAGGCT CCGGCTCGGG ATCTGAGACT TGTGAACAGG ACCGATGTCG CCGATATGGA
    GGAACGTGGG ACGATGACAT GGAGGACGAG CGATGTGTTT GTGAACTAAA CTGTCAGATT
    GTGCTGCACA GCCCGGTGTG CGGCTCTGAT GGAAACACGT ACTCCAGCGA CTGTGAGCTC
    AAGAAGACCA GGTGCGAAAA GCAAATAGAC CTGAGTGTCC AAAGCCAAGG GCCCTGTCCA
    ACTATCGTAT CACCACCTCC AGAGCTTGTT GCCTCCCAGC ACTGTAGCCA AACAGTTTAT
    GGATGTTGTG AAGATAACAA GACCGCTGCC CTGGGTGTTG GACTGGCTGG ATGTCCTAGT
    ATGTGTCAGT GCAATCATTA CGGCTCATAC GGAGGCACAT GTGACCCCTC GACTGGCCAG
    TGTTCCTGTA AACCTGGTGT CGGTGGACTG AAGTGCGACC GCTGTGAGCC CGGATTTTGG
    AACTTCCGTG GCATTGTCAC AGAAAACATG AGCGGCTGCA CACCGTGCAA CTGTGATGGA
    GTGGGTTCGG TTCGTGATGA CTGCGAGCAG ATGTCAGGCC TGTGCTCCTG TAAGCCTGGA
    GTTAAGGGCA TGAAATGCAA CGTGTGCCCT GATGGCAGCA AGATGGGAGT CAACGGCTGT
    GACAAAGGAC CTAAGGCTCC CAAGTCCTGT GCTGAGCTTG TGTGCCACTT TGGGTCTTCA
    TGCGTGGAGG TGAATGGACA GGCTCACTGT GAGTGTCCTT CACCAGATTG TGATGAGAAA
    AACAAGACCA AGGTATGTGG TTCTGATGGG GTCACCTATG CAGACCGGTG CCAGCTGAAG
    ACCATCGCAT GCCGACAAGA TAAAGAGATC AAGGTGGAGC ATTTAGGCCA GTGTAAAGAA
    TCCATTGAGC TGACGGCCAA ACCCACCCCC TTCCCAACCA CCCACCGCAC CACCCCTCCC
    ACCGCTCACG TCAAGGTCAC CAAACAGCGA CATGGCCACC GCACCACCAC TCCGGACAGC
    TATGTGGTGG AGGCAATGCC TCCACCGAGG TTGGATGGAG CGCGAAAGTT TTCCAGCCCT
    TTCACCACCC ACCGTCCCAC TACACCCTAC AAACCACTCC GCACCTCATC CCCACCTCTG
    GACTTTGACG AGTCGGGCAG TGGAGAGCCC AGTGGGGATG ATGAGATGGT GAGCGGAATG
    GAGGAGAGCG GAGAAGAGCC ACTTGGTACA ACAGTAGCTA CTACCACTCT TCCCACAGCT
    GAGGAGCGAT CGTCCTGTGA CAACACAGCG TTCGGCTGCT GTCCCGATGG GAAGACGGCC
    GCCGTCAACT CAGAGGGCAC AAACTGCCCA TCCACCATGC GTTTCAGCGG CTTCCTGCAC
    CTGGATAAGG TTGAGGGTCA GGAGGTCTTC TACACGCCTG AGATGGAGGA CCCCAAATCG
    GAACTGTTTG GTGAAACGGC CCGAAGCATC GAGAGCGCTA TGAATGAGTT GTTCCGCAAG
    TCTGATGTCC AGAAAGACTT CCAGAGTGTC CGTGTGAGGA ATCTATCCCC CAGCAACTCC
    ATAATAGCAC ATTTCGAGGC TCACTTCGAC CCAGACACAA GGTTCAATGT GGGGGATATT
    GAGGGAGCCC TTCTCAAACA GCTTAAGGCG TCTAAAGACA CTGGCATCGT TGTGAAGAAA
    CCAGAGGAAG AGAACATTCG CATCAACAAC TATGGTCTTT CAACTGTTCC TTTCTTTACC
    ACCACAACAA CCACTGTGGC ATCTGTCACG ACTCCCACTA CCACCACCAC CACAATGTCT
    CCCAGTTCTG CTTATACCAC TCTCCGGCCC ATCACTACTA GACGACCCTT CACAAGAACC
    ACCACGCCAG CACCAGCCAC CACCACTGCA CCACATGTGC CCAAGACCAC AGTTTCGCTT
    ATGAACAGAC TAGGGCCCCA CGCTCACAGT CCATGTGACT CTCAGCCCTG CCGACATGGA
    GGAACCTGTG AAGAAGACGA CAATGACTTC ACCTGTATCT GTCCAGCAGG ACGAGGAGGA
    GCCGTGTGTG AGAAAGTTAT AAAGTACTTC ATACCATCCT TTGGTGGAAA GTCTTACATG
    GCGTTCCAGA CCATGAAGGC CTACCATACT GTACGCATTG CCATGGAGTT CAGGGCTTCG
    GAAATGACTG GGATATTGCT CTACAATGGG CAAAAAAGCA AAAAGGACTT TCTTTCTCTG
    ACTCTAGTTG AAGGATATGT GGAGCTCAGG TTTAACACTG GTTCAGGGAC TGCTTCAGTT
    ATCAGTAAAG TCCTGGTAAA GCCGGGTCGC TGGCATCAGC TTGTTGTAGT ACGTAACAGG
    CGCAATGCCA TGCTGAGCGT GGACAATGAG CCCAGCGTTG AAGGACAAAG TCCTCCAGGA
    ACAGATGGAC TCAACCTGGA CACAGACCTT TTCATCGGCG GAGTGCTTGA TGACATGAGT
    GCAGACGTAA AGGAGAGGAC ATCAGTTTCA GCAGGTCTGG TGGGCTGCAT CCGCATGCTG
    GACGTGAACA ACCTTGTCTA TAATCTGCAA GAGAATGGCG GCAGCGTTCT GTACGGTTCG
    GCCATTGGCG AGTGTGGCAA CAACCCATGC CTGCCAAACC CCTGCAAGAA CGGAGCGTCC
    TGTCAGGTCA AAGAAGCCGA GATTTTCCAC TGCAAATGTG TCAACGGTTT CTCTGGCCCG
    ACCTGCGCTG ATGCCCACAA TCCCTGCGAC CCCAATAAAT GCCACCCGCC ATCACGGTGC
    CAGGTCCTTC CAGAGGGAGG TTACAAATGC GAATGTCCGA TGGGACGTGA AGGCAGGCAC
    TGTGAAAAAG TGTCAGACAA GGGAGGCGCT TTCATTCCTT ACTTCACTGG AGATTCTTTT
    CTGGAGCTCA AAGGCCTCCA TACGTATAAC CAGGATCTCA GGCAAAAATT CAGCATGACT
    ATCGTTCTAC TGGCCAATGA CTCCAAAGGC ATGATCTTCT ACAATGGTCA GAAGACAGAC
    GGCAAAGGGG ATTTCATCTC GCTCTCTCTT AATGACGGCA TCCTGGAGTT TAGATATGAC
    CTGGGCAAAG GGCCAGCTGT CATTAGGAGT AAAGAAAAAA TCAAGCTGAA CGAGTGGAAC
    ACAGTGAATT TGGAACGGGC CAGCAGAAAA GGAGAGATTA GCATTAACGG CAAAGACCCT
    GTCAGAGGAG AGGCGCCGAA ATCACGCAAG AATCAGCACA CGGATCTCAA TCTGAAGGAA
    TCGCTCTTTG TGGGTGGAGC TCCTGATTTC CGCAAAGTAG CCAGAGCTGC TGCGATCAAA
    GATGGCTTCA AAGGGGCCAT TCAGAAGATT ACTTTGATGG GAATTCCAAT CCTGAAAGCT
    GATAATGCCC TTTCGTCCAC TGACGTGTCC ATGTATCCTA ATCATCCCTG CTCCAAAGAC
    GTTTGTGAGA ATGGAGGCCG CTGCAACCCC CAGCTGAACA GCTATGAGTG CGCCTGCCGC
    CACGGATTCT CCGGACACCA CTGCCAGGAC ACAATATTTG AGAAGTCCGC TGGGGACACG
    GAGGCCATTG CGTTTGATGG TCATACATTC ATCGAATATC ACAACGGAGT CACCAAAAGC
    CAACTGACCA ATGAGATCCC AGACCCTGAG TCACTGGAGA ACCCATCTGA CCAGAGCGAG
    AAAGCCCTGC TGGTGAATAA ATTCGAGCTG AGCATCAGGA CGGAGGCTAC GCATGGTCTG
    ATTTTGTGGA GCGGAAAGGG AGTTGAGCGC TCTGACTACA TCGCCCTCGC CATTGTGGAT
    GGAAGAGTAC AGATGACCTA TGACCTCGGC TCCAAGCCTG TGGTGCTGCG CTCCACTGTA
    CGGGTCAACA CCAACCACTG GATACGAATT AAGGCCAGCA GAGCGCTCAG AGATGGCTCT
    CTGCAGGTTG GTAATGAGGC AGCTGTCACT GGGTCTTCAC CCTTGGCTGC CACACAGCTG
    GACACAGACG GGGCTCTCTG GTTGGGTGGA CTGGAGGAGC TGTCTGTGGC TCGACGGCTG
    CCCAAAGCTT ACAGTACAGG CTTCATTGGC TGCGTCAAAG ACGTGATAGT GGACGGCGTG
    GAGCTGCATC TGGTGGAGGA CGCCTTAAAT ACTCCCAAAA TATCACACTG TTCTGCCAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325509.1
    CDS1645..7527
    Translation

    Target ORF information:

    RefSeq Version XM_021469834.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469834.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGACCAGTT GTCAGTACCC CACGCCGCCC CCAGAGCGGG ACCGCATGTA CCAGCACAAA 
    GTGTCGCTGG TCGTGCGATA CTTCATGATT CCTTGCAACA TCTGTCTCAT ACTGCTGGCC
    ACATCCACGC TCGGCTTTGC TGTACTCCTC TTCCTCAATA ACTGTAGGTC ACAGTTAGTG
    AAACATGACA TGCAGCATAA ACCTGTTCAC TTCACACCGG CACCTCCTCC TGATGGTTGC
    AGAGGGAAGT TGTGCGGATT TGGAGCGGTG TGTGAGAGAG ACCAAGCTGA CCCATCTAAA
    GGAGAGTGTG TGTGTAAGAA GATTGTGTGT ACATCTGTGG TGGCGCCAGT GTGCGGATCT
    GACTCCTCCA CCTATTCCAA TGAGTGTGAG CTGGAGAAGG CTCAGTGCAA TACACAGAGA
    CGCATCAAGG TGATGCGCAA AGGCCCCTGC GCCCTCAAGG ACCCGTGCAG TGAGGTAACC
    TGTAGCTTTG GCAGCACCTG CATCCAGTCA TCGGATGGTC TGTCCGCCAA GTGTATGTGT
    CCTCTGAGCT GTGAAAACGT CCCCAAGCAT GTGGTGTGTG GCAGTGACGG CCTGGACTAC
    CAAAGCGAAT GTGAACTCAA CATGAAGGCC TGCGCCACTC AGAAGAACAT CCGTGTGCAT
    CACCAGGGCC GCTGCGATCC TTGCAGTGAC ACTCTGAACA GCTTAAGCAC AGCCTGTCGA
    GTCAACCCAA AGACCTACCA GCAGATTACC TTTGCCCCGG CTGAGTCTTG TCCGCCTGAC
    GAAATGCCCA TCTGTGCCAG CGACGGACAC ACCTACGAGA GCATGTGTCA GATGGAGCGC
    ACTGCCCTGA AGAACAACCT GGAGCTCAAA AAGGTCTCTT TAGGCAGCTG CAAGGAAAAA
    GGGGGCTGCC CGAATGGTTG TAAGTTCAAC GCCATATGTT TGCTGGATAA TGGAGAGTTT
    CGGTGCTCCT GCGATCCCAT ACAATGCGAC GGCACGTACA AGCCCCTGTG CGGAAAGGAT
    GGCAAGACAT ACCCCAACGA CTGCGAGAGG AAACTGCAGG AATGCCGGAC CCAAAAAGAC
    ATCCCTGTCA AACAACAAGG GCCCTGTGAT CTTAACCTGG AGAGCCCCTG CCTGAAGAAG
    ACCTGCGAGT TTGGAGCCGT GTGCGTGGTG AAGAACAGCG AAGCAGTGTG TGAATGCTCA
    GACGCCTGTC CGCAGGACCA AGACCCTGTT TGCGGCAGCG ATGGGCACAC GTACAGCAGC
    TCATGTCAAA TGAAGGCCAT GGGATGCGCT CTTCAGAAAC AGATACAGAT GCAACACAAA
    GGACCATGTG CAGATGAGTC CTGCACCAAC TGCTCGTTTG GCGCGATCTG TGATGCCCAG
    ACTGGCCGGT GCGTGTGCCC TAAAGGCTGT TTGGAAACAC GGCAGCCCGT ATGCGGCAGC
    GACGGCACCA CGTATGACAA CGAGTGCAAG CTCAACGTCC AAGCCTGCAC TAAACAGCTG
    GATCTCAAGG TGGTGGCCCA TGGAGAATGC AAAACCTGTG GCAGCACTGT GTGTAGCTGG
    GGGTCGCAGT GCGTGAACAA CAAGTGTGAG TGTCAGCAGT GCACTGGTCA GCCGGTGAAA
    CCCGTGTGTG GCAGCGACGG CAACACATAC AACAACGACT GCGAGCTGAG ACTGGCCTCC
    TGTCAGAAAC AGAGGAAGAT AGAGGTGGCC AAGCCTGGCG TCTGCGATGA AGACTGTGGT
    TCAGGAGGCT CCGGCTCGGG ATCTGAGACT TGTGAACAGG ACCGATGTCG CCGATATGGA
    GGAACGTGGG ACGATGACAT GGAGGACGAG CGATGTGTTT GTGAACTAAA CTGTCAGATT
    GTGCTGCACA GCCCGGTGTG CGGCTCTGAT GGAAACACGT ACTCCAGCGA CTGTGAGCTC
    AAGAAGACCA GGTGCGAAAA GCAAATAGAC CTGAGTGTCC AAAGCCAAGG GCCCTGTCCA
    ACTATCGTAT CACCACCTCC AGAGCTTGTT GCCTCCCAGC ACTGTAGCCA AACAGTTTAT
    GGATGTTGTG AAGATAACAA GACCGCTGCC CTGGGTGTTG GACTGGCTGG ATGTCCTAGT
    ATGTGTCAGT GCAATCATTA CGGCTCATAC GGAGGCACAT GTGACCCCTC GACTGGCCAG
    TGTTCCTGTA AACCTGGTGT CGGTGGACTG AAGTGCGACC GCTGTGAGCC CGGATTTTGG
    AACTTCCGTG GCATTGTCAC AGAAAACATG AGCGGCTGCA CACCGTGCAA CTGTGATGGA
    GTGGGTTCGG TTCGTGATGA CTGCGAGCAG ATGTCAGGCC TGTGCTCCTG TAAGCCTGGA
    GTTAAGGGCA TGAAATGCAA CGTGTGCCCT GATGGCAGCA AGATGGGAGT CAACGGCTGT
    GACAAAGGAC CTAAGGCTCC CAAGTCCTGT GCTGAGCTTG TGTGCCACTT TGGGTCTTCA
    TGCGTGGAGG TGAATGGACA GGCTCACTGT GAGTGTCCTT CACCAGATTG TGATGAGAAA
    AACAAGACCA AGGTATGTGG TTCTGATGGG GTCACCTATG CAGACCGGTG CCAGCTGAAG
    ACCATCGCAT GCCGACAAGA TAAAGAGATC AAGGTGGAGC ATTTAGGCCA GTGTAAAGAA
    TCCATTGAGC TGACGGCCAA ACCCACCCCC TTCCCAACCA CCCACCGCAC CACCCCTCCC
    ACCGCTCACG TCAAGGTCAC CAAACAGCGA CATGGCCACC GCACCACCAC TCCGGACAGC
    TATGTGGTGG AGGCAATGCC TCCACCGAGG TTGGATGGAG CGCGAAAGTT TTCCAGCCCT
    TTCACCACCC ACCGTCCCAC TACACCCTAC AAACCACTCC GCACCTCATC CCCACCTCTG
    GACTTTGACG AGTCGGGCAG TGGAGAGCCC AGTGGGGATG ATGAGATGGT GAGCGGAATG
    GAGGAGAGCG GAGAAGAGCC ACTTGGTACA ACAGTAGCTA CTACCACTCT TCCCACAGCT
    GAGGAGCGAT CGTCCTGTGA CAACACAGCG TTCGGCTGCT GTCCCGATGG GAAGACGGCC
    GCCGTCAACT CAGAGGGCAC AAACTGCCCA TCCACCATGC GTTTCAGCGG CTTCCTGCAC
    CTGGATAAGG TTGAGGGTCA GGAGGTCTTC TACACGCCTG AGATGGAGGA CCCCAAATCG
    GAACTGTTTG GTGAAACGGC CCGAAGCATC GAGAGCGCTA TGAATGAGTT GTTCCGCAAG
    TCTGATGTCC AGAAAGACTT CCAGAGTGTC CGTGTGAGGA ATCTATCCCC CAGCAACTCC
    ATAATAGCAC ATTTCGAGGC TCACTTCGAC CCAGACACAA GGTTCAATGT GGGGGATATT
    GAGGGAGCCC TTCTCAAACA GCTTAAGGCG TCTAAAGACA CTGGCATCGT TGTGAAGAAA
    CCAGAGGAAG AGAACATTCG CATCAACAAC TATGGTCTTT CAACTGTTCC TTTCTTTACC
    ACCACAACAA CCACTGTGGC ATCTGTCACG ACTCCCACTA CCACCACCAC CACAATGTCT
    CCCAGTTCTG CTTATACCAC TCTCCGGCCC ATCACTACTA GACGACCCTT CACAAGAACC
    ACCACGCCAG CACCAGCCAC CACCACTGCA CCACATGTGC CCAAGACCAC AGTTTCGCTT
    ATGAACAGAC TAGGGCCCCA CGCTCACAGT CCATGTGACT CTCAGCCCTG CCGACATGGA
    GGAACCTGTG AAGAAGACGA CAATGACTTC ACCTGTATCT GTCCAGCAGG ACGAGGAGGA
    GCCGTGTGTG AGAAAGTTAT AAAGTACTTC ATACCATCCT TTGGTGGAAA GTCTTACATG
    GCGTTCCAGA CCATGAAGGC CTACCATACT GTACGCATTG CCATGGAGTT CAGGGCTTCG
    GAAATGACTG GGATATTGCT CTACAATGGG CAAAAAAGCA AAAAGGACTT TCTTTCTCTG
    ACTCTAGTTG AAGGATATGT GGAGCTCAGG TTTAACACTG GTTCAGGGAC TGCTTCAGTT
    ATCAGTAAAG TCCTGGTAAA GCCGGGTCGC TGGCATCAGC TTGTTGTAGT ACGTAACAGG
    CGCAATGCCA TGCTGAGCGT GGACAATGAG CCCAGCGTTG AAGGACAAAG TCCTCCAGGA
    ACAGATGGAC TCAACCTGGA CACAGACCTT TTCATCGGCG GAGTGCTTGA TGACATGAGT
    GCAGACGTAA AGGAGAGGAC ATCAGTTTCA GCAGGTCTGG TGGGCTGCAT CCGCATGCTG
    GACGTGAACA ACCTTGTCTA TAATCTGCAA GAGAATGGCG GCAGCGTTCT GTACGGTTCG
    GCCATTGGCG AGTGTGGCAA CAACCCATGC CTGCCAAACC CCTGCAAGAA CGGAGCGTCC
    TGTCAGGTCA AAGAAGCCGA GATTTTCCAC TGCAAATGTG TCAACGGTTT CTCTGGCCCG
    ACCTGCGCTG ATGCCCACAA TCCCTGCGAC CCCAATAAAT GCCACCCGCC ATCACGGTGC
    CAGGTCCTTC CAGAGGGAGG TTACAAATGC GAATGTCCGA TGGGACGTGA AGGCAGGCAC
    TGTGAAAAAG TGTCAGACAA GGGAGGCGCT TTCATTCCTT ACTTCACTGG AGATTCTTTT
    CTGGAGCTCA AAGGCCTCCA TACGTATAAC CAGGATCTCA GGCAAAAATT CAGCATGACT
    ATCGTTCTAC TGGCCAATGA CTCCAAAGGC ATGATCTTCT ACAATGGTCA GAAGACAGAC
    GGCAAAGGGG ATTTCATCTC GCTCTCTCTT AATGACGGCA TCCTGGAGTT TAGATATGAC
    CTGGGCAAAG GGCCAGCTGT CATTAGGAGT AAAGAAAAAA TCAAGCTGAA CGAGTGGAAC
    ACAGTGAATT TGGAACGGGC CAGCAGAAAA GGAGAGATTA GCATTAACGG CAAAGACCCT
    GTCAGAGGAG AGGCGCCGAA ATCACGCAAG AATCAGCACA CGGATCTCAA TCTGAAGGAA
    TCGCTCTTTG TGGGTGGAGC TCCTGATTTC CGCAAAGTAG CCAGAGCTGC TGCGATCAAA
    GATGGCTTCA AAGGGGCCAT TCAGAAGATT ACTTTGATGG GAATTCCAAT CCTGAAAGCT
    GATAATGCCC TTTCGTCCAC TGACGTGTCC ATGTATCCTA ATCATCCCTG CTCCAAAGAC
    GTTTGTGAGA ATGGAGGCCG CTGCAACCCC CAGCTGAACA GCTATGAGTG CGCCTGCCGC
    CACGGATTCT CCGGACACCA CTGCCAGGAC ACAATATTTG AGAAGTCCGC TGGGGACACG
    GAGGCCATTG CGTTTGATGG TCATACATTC ATCGAATATC ACAACGGAGT CACCAAAAGC
    CAACTGACCA ATGAGATCCC AGACCCTGAG TCACTGGAGA ACCCATCTGA CCAGAGCGAG
    AAAGCCCTGC TGGTGAATAA ATTCGAGCTG AGCATCAGGA CGGAGGCTAC GCATGGTCTG
    ATTTTGTGGA GCGGAAAGGG AGTTGAGCGC TCTGACTACA TCGCCCTCGC CATTGTGGAT
    GGAAGAGTAC AGATGACCTA TGACCTCGGC TCCAAGCCTG TGGTGCTGCG CTCCACTGTA
    CGGGTCAACA CCAACCACTG GATACGAATT AAGGCCAGCA GAGCGCTCAG AGATGGCTCT
    CTGCAGGTTG GTAATGAGGC AGCTGTCACT GGGTCTTCAC CCTTGGCTGC CACACAGCTG
    GACACAGACG GGGCTCTCTG GTTGGGTGGA CTGGAGGAGC TGTCTGTGGC TCGACGGCTG
    CCCAAAGCTT ACAGTACAGG CTTCATTGGC TGCGTCAAAG ACGTGATAGT GGACGGCGTG
    GAGCTGCATC TGGTGGAGGA CGCCTTAAAT ACTCCCAAAA TATCACACTG TTCTGCCAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151481
    Clone ID Related Accession (Same CDS sequence) XM_021469835.1
    Accession Version XM_021469835.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5850bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product agrin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGACCAGTT GTCAGTACCC CACGCCGCCC CCAGAGCGGG ACCGCATGTA CCAGCACAAA 
    GTGTCGCTGG TCGTGCGATA CTTCATGATT CCTTGCAACA TCTGTCTCAT ACTGCTGGCC
    ACATCCACGC TCGGCTTTGC TGTACTCCTC TTCCTCAATA ACTATAAACC TGTTCACTTC
    ACACCGGCAC CTCCTCCTGA TGGTTGCAGA GGGAAGTTGT GCGGATTTGG AGCGGTGTGT
    GAGAGAGACC AAGCTGACCC ATCTAAAGGA GAGTGTGTGT GTAAGAAGAT TGTGTGTACA
    TCTGTGGTGG CGCCAGTGTG CGGATCTGAC TCCTCCACCT ATTCCAATGA GTGTGAGCTG
    GAGAAGGCTC AGTGCAATAC ACAGAGACGC ATCAAGGTGA TGCGCAAAGG CCCCTGCGCC
    CTCAAGGACC CGTGCAGTGA GGTAACCTGT AGCTTTGGCA GCACCTGCAT CCAGTCATCG
    GATGGTCTGT CCGCCAAGTG TATGTGTCCT CTGAGCTGTG AAAACGTCCC CAAGCATGTG
    GTGTGTGGCA GTGACGGCCT GGACTACCAA AGCGAATGTG AACTCAACAT GAAGGCCTGC
    GCCACTCAGA AGAACATCCG TGTGCATCAC CAGGGCCGCT GCGATCCTTG CAGTGACACT
    CTGAACAGCT TAAGCACAGC CTGTCGAGTC AACCCAAAGA CCTACCAGCA GATTACCTTT
    GCCCCGGCTG AGTCTTGTCC GCCTGACGAA ATGCCCATCT GTGCCAGCGA CGGACACACC
    TACGAGAGCA TGTGTCAGAT GGAGCGCACT GCCCTGAAGA ACAACCTGGA GCTCAAAAAG
    GTCTCTTTAG GCAGCTGCAA GGAAAAAGGG GGCTGCCCGA ATGGTTGTAA GTTCAACGCC
    ATATGTTTGC TGGATAATGG AGAGTTTCGG TGCTCCTGCG ATCCCATACA ATGCGACGGC
    ACGTACAAGC CCCTGTGCGG AAAGGATGGC AAGACATACC CCAACGACTG CGAGAGGAAA
    CTGCAGGAAT GCCGGACCCA AAAAGACATC CCTGTCAAAC AACAAGGGCC CTGTGATCTT
    AACCTGGAGA GCCCCTGCCT GAAGAAGACC TGCGAGTTTG GAGCCGTGTG CGTGGTGAAG
    AACAGCGAAG CAGTGTGTGA ATGCTCAGAC GCCTGTCCGC AGGACCAAGA CCCTGTTTGC
    GGCAGCGATG GGCACACGTA CAGCAGCTCA TGTCAAATGA AGGCCATGGG ATGCGCTCTT
    CAGAAACAGA TACAGATGCA ACACAAAGGA CCATGTGCAG ATGAGTCCTG CACCAACTGC
    TCGTTTGGCG CGATCTGTGA TGCCCAGACT GGCCGGTGCG TGTGCCCTAA AGGCTGTTTG
    GAAACACGGC AGCCCGTATG CGGCAGCGAC GGCACCACGT ATGACAACGA GTGCAAGCTC
    AACGTCCAAG CCTGCACTAA ACAGCTGGAT CTCAAGGTGG TGGCCCATGG AGAATGCAAA
    ACCTGTGGCA GCACTGTGTG TAGCTGGGGG TCGCAGTGCG TGAACAACAA GTGTGAGTGT
    CAGCAGTGCA CTGGTCAGCC GGTGAAACCC GTGTGTGGCA GCGACGGCAA CACATACAAC
    AACGACTGCG AGCTGAGACT GGCCTCCTGT CAGAAACAGA GGAAGATAGA GGTGGCCAAG
    CCTGGCGTCT GCGATGAAGA CTGTGGTTCA GGAGGCTCCG GCTCGGGATC TGAGACTTGT
    GAACAGGACC GATGTCGCCG ATATGGAGGA ACGTGGGACG ATGACATGGA GGACGAGCGA
    TGTGTTTGTG AACTAAACTG TCAGATTGTG CTGCACAGCC CGGTGTGCGG CTCTGATGGA
    AACACGTACT CCAGCGACTG TGAGCTCAAG AAGACCAGGT GCGAAAAGCA AATAGACCTG
    AGTGTCCAAA GCCAAGGGCC CTGTCCAACT ATCGTATCAC CACCTCCAGA GCTTGTTGCC
    TCCCAGCACT GTAGCCAAAC AGTTTATGGA TGTTGTGAAG ATAACAAGAC CGCTGCCCTG
    GGTGTTGGAC TGGCTGGATG TCCTAGTATG TGTCAGTGCA ATCATTACGG CTCATACGGA
    GGCACATGTG ACCCCTCGAC TGGCCAGTGT TCCTGTAAAC CTGGTGTCGG TGGACTGAAG
    TGCGACCGCT GTGAGCCCGG ATTTTGGAAC TTCCGTGGCA TTGTCACAGA AAACATGAGC
    GGCTGCACAC CGTGCAACTG TGATGGAGTG GGTTCGGTTC GTGATGACTG CGAGCAGATG
    TCAGGCCTGT GCTCCTGTAA GCCTGGAGTT AAGGGCATGA AATGCAACGT GTGCCCTGAT
    GGCAGCAAGA TGGGAGTCAA CGGCTGTGAC AAAGGACCTA AGGCTCCCAA GTCCTGTGCT
    GAGCTTGTGT GCCACTTTGG GTCTTCATGC GTGGAGGTGA ATGGACAGGC TCACTGTGAG
    TGTCCTTCAC CAGATTGTGA TGAGAAAAAC AAGACCAAGG TATGTGGTTC TGATGGGGTC
    ACCTATGCAG ACCGGTGCCA GCTGAAGACC ATCGCATGCC GACAAGATAA AGAGATCAAG
    GTGGAGCATT TAGGCCAGTG TAAAGAATCC ATTGAGCTGA CGGCCAAACC CACCCCCTTC
    CCAACCACCC ACCGCACCAC CCCTCCCACC GCTCACGTCA AGGTCACCAA ACAGCGACAT
    GGCCACCGCA CCACCACTCC GGACAGCTAT GTGGTGGAGG CAATGCCTCC ACCGAGGTTG
    GATGGAGCGC GAAAGTTTTC CAGCCCTTTC ACCACCCACC GTCCCACTAC ACCCTACAAA
    CCACTCCGCA CCTCATCCCC ACCTCTGGAC TTTGACGAGT CGGGCAGTGG AGAGCCCAGT
    GGGGATGATG AGATGGTGAG CGGAATGGAG GAGAGCGGAG AAGAGCCACT TGGTACAACA
    GTAGCTACTA CCACTCTTCC CACAGCTGAG GAGCGATCGT CCTGTGACAA CACAGCGTTC
    GGCTGCTGTC CCGATGGGAA GACGGCCGCC GTCAACTCAG AGGGCACAAA CTGCCCATCC
    ACCATGCGTT TCAGCGGCTT CCTGCACCTG GATAAGGTTG AGGGTCAGGA GGTCTTCTAC
    ACGCCTGAGA TGGAGGACCC CAAATCGGAA CTGTTTGGTG AAACGGCCCG AAGCATCGAG
    AGCGCTATGA ATGAGTTGTT CCGCAAGTCT GATGTCCAGA AAGACTTCCA GAGTGTCCGT
    GTGAGGAATC TATCCCCCAG CAACTCCATA ATAGCACATT TCGAGGCTCA CTTCGACCCA
    GACACAAGGT TCAATGTGGG GGATATTGAG GGAGCCCTTC TCAAACAGCT TAAGGCGTCT
    AAAGACACTG GCATCGTTGT GAAGAAACCA GAGGAAGAGA ACATTCGCAT CAACAACTAT
    GGTCTTTCAA CTGTTCCTTT CTTTACCACC ACAACAACCA CTGTGGCATC TGTCACGACT
    CCCACTACCA CCACCACCAC AATGTCTCCC AGTTCTGCTT ATACCACTCT CCGGCCCATC
    ACTACTAGAC GACCCTTCAC AAGAACCACC ACGCCAGCAC CAGCCACCAC CACTGCACCA
    CATGTGCCCA AGACCACAGT TTCGCTTATG AACAGACTAG GGCCCCACGC TCACAGTCCA
    TGTGACTCTC AGCCCTGCCG ACATGGAGGA ACCTGTGAAG AAGACGACAA TGACTTCACC
    TGTATCTGTC CAGCAGGACG AGGAGGAGCC GTGTGTGAGA AAGTTATAAA GTACTTCATA
    CCATCCTTTG GTGGAAAGTC TTACATGGCG TTCCAGACCA TGAAGGCCTA CCATACTGTA
    CGCATTGCCA TGGAGTTCAG GGCTTCGGAA ATGACTGGGA TATTGCTCTA CAATGGGCAA
    AAAAGCAAAA AGGACTTTCT TTCTCTGACT CTAGTTGAAG GATATGTGGA GCTCAGGTTT
    AACACTGGTT CAGGGACTGC TTCAGTTATC AGTAAAGTCC TGGTAAAGCC GGGTCGCTGG
    CATCAGCTTG TTGTAGTACG TAACAGGCGC AATGCCATGC TGAGCGTGGA CAATGAGCCC
    AGCGTTGAAG GACAAAGTCC TCCAGGAACA GATGGACTCA ACCTGGACAC AGACCTTTTC
    ATCGGCGGAG TGCTTGATGA CATGAGTGCA GACGTAAAGG AGAGGACATC AGTTTCAGCA
    GGTCTGGTGG GCTGCATCCG CATGCTGGAC GTGAACAACC TTGTCTATAA TCTGCAAGAG
    AATGGCGGCA GCGTTCTGTA CGGTTCGGCC ATTGGCGAGT GTGGCAACAA CCCATGCCTG
    CCAAACCCCT GCAAGAACGG AGCGTCCTGT CAGGTCAAAG AAGCCGAGAT TTTCCACTGC
    AAATGTGTCA ACGGTTTCTC TGGCCCGACC TGCGCTGATG CCCACAATCC CTGCGACCCC
    AATAAATGCC ACCCGCCATC ACGGTGCCAG GTCCTTCCAG AGGGAGGTTA CAAATGCGAA
    TGTCCGATGG GACGTGAAGG CAGGCACTGT GAAAAAGTGT CAGACAAGGG AGGCGCTTTC
    ATTCCTTACT TCACTGGAGA TTCTTTTCTG GAGCTCAAAG GCCTCCATAC GTATAACCAG
    GATCTCAGGC AAAAATTCAG CATGACTATC GTTCTACTGG CCAATGACTC CAAAGGCATG
    ATCTTCTACA ATGGTCAGAA GACAGACGGC AAAGGGGATT TCATCTCGCT CTCTCTTAAT
    GACGGCATCC TGGAGTTTAG ATATGACCTG GGCAAAGGGC CAGCTGTCAT TAGGAGTAAA
    GAAAAAATCA AGCTGAACGA GTGGAACACA GTGAATTTGG AACGGGCCAG CAGAAAAGGA
    GAGATTAGCA TTAACGGCAA AGACCCTGTC AGAGGAGAGG CGCCGAAATC ACGCAAGAAT
    CAGCACACGG ATCTCAATCT GAAGGAATCG CTCTTTGTGG GTGGAGCTCC TGATTTCCGC
    AAAGTAGCCA GAGCTGCTGC GATCAAAGAT GGCTTCAAAG GGGCCATTCA GAAGATTACT
    TTGATGGGAA TTCCAATCCT GAAAGCTGAT AATGCCCTTT CGTCCACTGA CGTGTCCATG
    TATCCTAATC ATCCCTGCTC CAAAGACGTT TGTGAGAATG GAGGCCGCTG CAACCCCCAG
    CTGAACAGCT ATGAGTGCGC CTGCCGCCAC GGATTCTCCG GACACCACTG CCAGGACACA
    ATATTTGAGA AGTCCGCTGG GGACACGGAG GCCATTGCGT TTGATGGTCA TACATTCATC
    GAATATCACA ACGGAGTCAC CAAAAGCCAA CTGACCAATG AGATCCCAGA CCCTGAGTCA
    CTGGAGAACC CATCTGACCA GAGCGAGAAA GCCCTGCTGG TGAATAAATT CGAGCTGAGC
    ATCAGGACGG AGGCTACGCA TGGTCTGATT TTGTGGAGCG GAAAGGGAGT TGAGCGCTCT
    GACTACATCG CCCTCGCCAT TGTGGATGGA AGAGTACAGA TGACCTATGA CCTCGGCTCC
    AAGCCTGTGG TGCTGCGCTC CACTGTACGG GTCAACACCA ACCACTGGAT ACGAATTAAG
    GCCAGCAGAG CGCTCAGAGA TGGCTCTCTG CAGGTTGGTA ATGAGGCAGC TGTCACTGGG
    TCTTCACCCT TGGCTGCCAC ACAGCTGGAC ACAGACGGGG CTCTCTGGTT GGGTGGACTG
    GAGGAGCTGT CTGTGGCTCG ACGGCTGCCC AAAGCTTACA GTACAGGCTT CATTGGCTGC
    GTCAAAGACG TGATAGTGGA CGGCGTGGAG CTGCATCTGG TGGAGGACGC CTTAAATACT
    CCCAAAATAT CACACTGTTC TGCCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325510.1
    CDS1645..7494
    Translation

    Target ORF information:

    RefSeq Version XM_021469835.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469835.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGACCAGTT GTCAGTACCC CACGCCGCCC CCAGAGCGGG ACCGCATGTA CCAGCACAAA 
    GTGTCGCTGG TCGTGCGATA CTTCATGATT CCTTGCAACA TCTGTCTCAT ACTGCTGGCC
    ACATCCACGC TCGGCTTTGC TGTACTCCTC TTCCTCAATA ACTATAAACC TGTTCACTTC
    ACACCGGCAC CTCCTCCTGA TGGTTGCAGA GGGAAGTTGT GCGGATTTGG AGCGGTGTGT
    GAGAGAGACC AAGCTGACCC ATCTAAAGGA GAGTGTGTGT GTAAGAAGAT TGTGTGTACA
    TCTGTGGTGG CGCCAGTGTG CGGATCTGAC TCCTCCACCT ATTCCAATGA GTGTGAGCTG
    GAGAAGGCTC AGTGCAATAC ACAGAGACGC ATCAAGGTGA TGCGCAAAGG CCCCTGCGCC
    CTCAAGGACC CGTGCAGTGA GGTAACCTGT AGCTTTGGCA GCACCTGCAT CCAGTCATCG
    GATGGTCTGT CCGCCAAGTG TATGTGTCCT CTGAGCTGTG AAAACGTCCC CAAGCATGTG
    GTGTGTGGCA GTGACGGCCT GGACTACCAA AGCGAATGTG AACTCAACAT GAAGGCCTGC
    GCCACTCAGA AGAACATCCG TGTGCATCAC CAGGGCCGCT GCGATCCTTG CAGTGACACT
    CTGAACAGCT TAAGCACAGC CTGTCGAGTC AACCCAAAGA CCTACCAGCA GATTACCTTT
    GCCCCGGCTG AGTCTTGTCC GCCTGACGAA ATGCCCATCT GTGCCAGCGA CGGACACACC
    TACGAGAGCA TGTGTCAGAT GGAGCGCACT GCCCTGAAGA ACAACCTGGA GCTCAAAAAG
    GTCTCTTTAG GCAGCTGCAA GGAAAAAGGG GGCTGCCCGA ATGGTTGTAA GTTCAACGCC
    ATATGTTTGC TGGATAATGG AGAGTTTCGG TGCTCCTGCG ATCCCATACA ATGCGACGGC
    ACGTACAAGC CCCTGTGCGG AAAGGATGGC AAGACATACC CCAACGACTG CGAGAGGAAA
    CTGCAGGAAT GCCGGACCCA AAAAGACATC CCTGTCAAAC AACAAGGGCC CTGTGATCTT
    AACCTGGAGA GCCCCTGCCT GAAGAAGACC TGCGAGTTTG GAGCCGTGTG CGTGGTGAAG
    AACAGCGAAG CAGTGTGTGA ATGCTCAGAC GCCTGTCCGC AGGACCAAGA CCCTGTTTGC
    GGCAGCGATG GGCACACGTA CAGCAGCTCA TGTCAAATGA AGGCCATGGG ATGCGCTCTT
    CAGAAACAGA TACAGATGCA ACACAAAGGA CCATGTGCAG ATGAGTCCTG CACCAACTGC
    TCGTTTGGCG CGATCTGTGA TGCCCAGACT GGCCGGTGCG TGTGCCCTAA AGGCTGTTTG
    GAAACACGGC AGCCCGTATG CGGCAGCGAC GGCACCACGT ATGACAACGA GTGCAAGCTC
    AACGTCCAAG CCTGCACTAA ACAGCTGGAT CTCAAGGTGG TGGCCCATGG AGAATGCAAA
    ACCTGTGGCA GCACTGTGTG TAGCTGGGGG TCGCAGTGCG TGAACAACAA GTGTGAGTGT
    CAGCAGTGCA CTGGTCAGCC GGTGAAACCC GTGTGTGGCA GCGACGGCAA CACATACAAC
    AACGACTGCG AGCTGAGACT GGCCTCCTGT CAGAAACAGA GGAAGATAGA GGTGGCCAAG
    CCTGGCGTCT GCGATGAAGA CTGTGGTTCA GGAGGCTCCG GCTCGGGATC TGAGACTTGT
    GAACAGGACC GATGTCGCCG ATATGGAGGA ACGTGGGACG ATGACATGGA GGACGAGCGA
    TGTGTTTGTG AACTAAACTG TCAGATTGTG CTGCACAGCC CGGTGTGCGG CTCTGATGGA
    AACACGTACT CCAGCGACTG TGAGCTCAAG AAGACCAGGT GCGAAAAGCA AATAGACCTG
    AGTGTCCAAA GCCAAGGGCC CTGTCCAACT ATCGTATCAC CACCTCCAGA GCTTGTTGCC
    TCCCAGCACT GTAGCCAAAC AGTTTATGGA TGTTGTGAAG ATAACAAGAC CGCTGCCCTG
    GGTGTTGGAC TGGCTGGATG TCCTAGTATG TGTCAGTGCA ATCATTACGG CTCATACGGA
    GGCACATGTG ACCCCTCGAC TGGCCAGTGT TCCTGTAAAC CTGGTGTCGG TGGACTGAAG
    TGCGACCGCT GTGAGCCCGG ATTTTGGAAC TTCCGTGGCA TTGTCACAGA AAACATGAGC
    GGCTGCACAC CGTGCAACTG TGATGGAGTG GGTTCGGTTC GTGATGACTG CGAGCAGATG
    TCAGGCCTGT GCTCCTGTAA GCCTGGAGTT AAGGGCATGA AATGCAACGT GTGCCCTGAT
    GGCAGCAAGA TGGGAGTCAA CGGCTGTGAC AAAGGACCTA AGGCTCCCAA GTCCTGTGCT
    GAGCTTGTGT GCCACTTTGG GTCTTCATGC GTGGAGGTGA ATGGACAGGC TCACTGTGAG
    TGTCCTTCAC CAGATTGTGA TGAGAAAAAC AAGACCAAGG TATGTGGTTC TGATGGGGTC
    ACCTATGCAG ACCGGTGCCA GCTGAAGACC ATCGCATGCC GACAAGATAA AGAGATCAAG
    GTGGAGCATT TAGGCCAGTG TAAAGAATCC ATTGAGCTGA CGGCCAAACC CACCCCCTTC
    CCAACCACCC ACCGCACCAC CCCTCCCACC GCTCACGTCA AGGTCACCAA ACAGCGACAT
    GGCCACCGCA CCACCACTCC GGACAGCTAT GTGGTGGAGG CAATGCCTCC ACCGAGGTTG
    GATGGAGCGC GAAAGTTTTC CAGCCCTTTC ACCACCCACC GTCCCACTAC ACCCTACAAA
    CCACTCCGCA CCTCATCCCC ACCTCTGGAC TTTGACGAGT CGGGCAGTGG AGAGCCCAGT
    GGGGATGATG AGATGGTGAG CGGAATGGAG GAGAGCGGAG AAGAGCCACT TGGTACAACA
    GTAGCTACTA CCACTCTTCC CACAGCTGAG GAGCGATCGT CCTGTGACAA CACAGCGTTC
    GGCTGCTGTC CCGATGGGAA GACGGCCGCC GTCAACTCAG AGGGCACAAA CTGCCCATCC
    ACCATGCGTT TCAGCGGCTT CCTGCACCTG GATAAGGTTG AGGGTCAGGA GGTCTTCTAC
    ACGCCTGAGA TGGAGGACCC CAAATCGGAA CTGTTTGGTG AAACGGCCCG AAGCATCGAG
    AGCGCTATGA ATGAGTTGTT CCGCAAGTCT GATGTCCAGA AAGACTTCCA GAGTGTCCGT
    GTGAGGAATC TATCCCCCAG CAACTCCATA ATAGCACATT TCGAGGCTCA CTTCGACCCA
    GACACAAGGT TCAATGTGGG GGATATTGAG GGAGCCCTTC TCAAACAGCT TAAGGCGTCT
    AAAGACACTG GCATCGTTGT GAAGAAACCA GAGGAAGAGA ACATTCGCAT CAACAACTAT
    GGTCTTTCAA CTGTTCCTTT CTTTACCACC ACAACAACCA CTGTGGCATC TGTCACGACT
    CCCACTACCA CCACCACCAC AATGTCTCCC AGTTCTGCTT ATACCACTCT CCGGCCCATC
    ACTACTAGAC GACCCTTCAC AAGAACCACC ACGCCAGCAC CAGCCACCAC CACTGCACCA
    CATGTGCCCA AGACCACAGT TTCGCTTATG AACAGACTAG GGCCCCACGC TCACAGTCCA
    TGTGACTCTC AGCCCTGCCG ACATGGAGGA ACCTGTGAAG AAGACGACAA TGACTTCACC
    TGTATCTGTC CAGCAGGACG AGGAGGAGCC GTGTGTGAGA AAGTTATAAA GTACTTCATA
    CCATCCTTTG GTGGAAAGTC TTACATGGCG TTCCAGACCA TGAAGGCCTA CCATACTGTA
    CGCATTGCCA TGGAGTTCAG GGCTTCGGAA ATGACTGGGA TATTGCTCTA CAATGGGCAA
    AAAAGCAAAA AGGACTTTCT TTCTCTGACT CTAGTTGAAG GATATGTGGA GCTCAGGTTT
    AACACTGGTT CAGGGACTGC TTCAGTTATC AGTAAAGTCC TGGTAAAGCC GGGTCGCTGG
    CATCAGCTTG TTGTAGTACG TAACAGGCGC AATGCCATGC TGAGCGTGGA CAATGAGCCC
    AGCGTTGAAG GACAAAGTCC TCCAGGAACA GATGGACTCA ACCTGGACAC AGACCTTTTC
    ATCGGCGGAG TGCTTGATGA CATGAGTGCA GACGTAAAGG AGAGGACATC AGTTTCAGCA
    GGTCTGGTGG GCTGCATCCG CATGCTGGAC GTGAACAACC TTGTCTATAA TCTGCAAGAG
    AATGGCGGCA GCGTTCTGTA CGGTTCGGCC ATTGGCGAGT GTGGCAACAA CCCATGCCTG
    CCAAACCCCT GCAAGAACGG AGCGTCCTGT CAGGTCAAAG AAGCCGAGAT TTTCCACTGC
    AAATGTGTCA ACGGTTTCTC TGGCCCGACC TGCGCTGATG CCCACAATCC CTGCGACCCC
    AATAAATGCC ACCCGCCATC ACGGTGCCAG GTCCTTCCAG AGGGAGGTTA CAAATGCGAA
    TGTCCGATGG GACGTGAAGG CAGGCACTGT GAAAAAGTGT CAGACAAGGG AGGCGCTTTC
    ATTCCTTACT TCACTGGAGA TTCTTTTCTG GAGCTCAAAG GCCTCCATAC GTATAACCAG
    GATCTCAGGC AAAAATTCAG CATGACTATC GTTCTACTGG CCAATGACTC CAAAGGCATG
    ATCTTCTACA ATGGTCAGAA GACAGACGGC AAAGGGGATT TCATCTCGCT CTCTCTTAAT
    GACGGCATCC TGGAGTTTAG ATATGACCTG GGCAAAGGGC CAGCTGTCAT TAGGAGTAAA
    GAAAAAATCA AGCTGAACGA GTGGAACACA GTGAATTTGG AACGGGCCAG CAGAAAAGGA
    GAGATTAGCA TTAACGGCAA AGACCCTGTC AGAGGAGAGG CGCCGAAATC ACGCAAGAAT
    CAGCACACGG ATCTCAATCT GAAGGAATCG CTCTTTGTGG GTGGAGCTCC TGATTTCCGC
    AAAGTAGCCA GAGCTGCTGC GATCAAAGAT GGCTTCAAAG GGGCCATTCA GAAGATTACT
    TTGATGGGAA TTCCAATCCT GAAAGCTGAT AATGCCCTTT CGTCCACTGA CGTGTCCATG
    TATCCTAATC ATCCCTGCTC CAAAGACGTT TGTGAGAATG GAGGCCGCTG CAACCCCCAG
    CTGAACAGCT ATGAGTGCGC CTGCCGCCAC GGATTCTCCG GACACCACTG CCAGGACACA
    ATATTTGAGA AGTCCGCTGG GGACACGGAG GCCATTGCGT TTGATGGTCA TACATTCATC
    GAATATCACA ACGGAGTCAC CAAAAGCCAA CTGACCAATG AGATCCCAGA CCCTGAGTCA
    CTGGAGAACC CATCTGACCA GAGCGAGAAA GCCCTGCTGG TGAATAAATT CGAGCTGAGC
    ATCAGGACGG AGGCTACGCA TGGTCTGATT TTGTGGAGCG GAAAGGGAGT TGAGCGCTCT
    GACTACATCG CCCTCGCCAT TGTGGATGGA AGAGTACAGA TGACCTATGA CCTCGGCTCC
    AAGCCTGTGG TGCTGCGCTC CACTGTACGG GTCAACACCA ACCACTGGAT ACGAATTAAG
    GCCAGCAGAG CGCTCAGAGA TGGCTCTCTG CAGGTTGGTA ATGAGGCAGC TGTCACTGGG
    TCTTCACCCT TGGCTGCCAC ACAGCTGGAC ACAGACGGGG CTCTCTGGTT GGGTGGACTG
    GAGGAGCTGT CTGTGGCTCG ACGGCTGCCC AAAGCTTACA GTACAGGCTT CATTGGCTGC
    GTCAAAGACG TGATAGTGGA CGGCGTGGAG CTGCATCTGG TGGAGGACGC CTTAAATACT
    CCCAAAATAT CACACTGTTC TGCCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151482
    Clone ID Related Accession (Same CDS sequence) XM_021469836.1
    Accession Version XM_021469836.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5844bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product agrin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGGTAC AACAGTAGCT
    ACTACCACTC TTCCCACAGC TGAGGAGCGA TCGTCCTGTG ACAACACAGC GTTCGGCTGC
    TGTCCCGATG GGAAGACGGC CGCCGTCAAC TCAGAGGGCA CAAACTGCCC ATCCACCATG
    CGTTTCAGCG GCTTCCTGCA CCTGGATAAG GTTGAGGGTC AGGAGGTCTT CTACACGCCT
    GAGATGGAGG ACCCCAAATC GGAACTGTTT GGTGAAACGG CCCGAAGCAT CGAGAGCGCT
    ATGAATGAGT TGTTCCGCAA GTCTGATGTC CAGAAAGACT TCCAGAGTGT CCGTGTGAGG
    AATCTATCCC CCAGCAACTC CATAATAGCA CATTTCGAGG CTCACTTCGA CCCAGACACA
    AGGTTCAATG TGGGGGATAT TGAGGGAGCC CTTCTCAAAC AGCTTAAGGC GTCTAAAGAC
    ACTGGCATCG TTGTGAAGAA ACCAGAGGAA GAGAACATTC GCATCAACAA CTATGGTCTT
    TCAACTGTTC CTTTCTTTAC CACCACAACA ACCACTGTGG CATCTGTCAC GACTCCCACT
    ACCACCACCA CCACAATGTC TCCCAGTTCT GCTTATACCA CTCTCCGGCC CATCACTACT
    AGACGACCCT TCACAAGAAC CACCACGCCA GCACCAGCCA CCACCACTGC ACCACATGTG
    CCCAAGACCA CAGTTTCGCT TATGAACAGA CTAGGGCCCC ACGCTCACAG TCCATGTGAC
    TCTCAGCCCT GCCGACATGG AGGAACCTGT GAAGAAGACG ACAATGACTT CACCTGTATC
    TGTCCAGCAG GACGAGGAGG AGCCGTGTGT GAGAAAGTTA TAAAGTACTT CATACCATCC
    TTTGGTGGAA AGTCTTACAT GGCGTTCCAG ACCATGAAGG CCTACCATAC TGTACGCATT
    GCCATGGAGT TCAGGGCTTC GGAAATGACT GGGATATTGC TCTACAATGG GCAAAAAAGC
    AAAAAGGACT TTCTTTCTCT GACTCTAGTT GAAGGATATG TGGAGCTCAG GTTTAACACT
    GGTTCAGGGA CTGCTTCAGT TATCAGTAAA GTCCTGGTAA AGCCGGGTCG CTGGCATCAG
    CTTGTTGTAG TACGTAACAG GCGCAATGCC ATGCTGAGCG TGGACAATGA GCCCAGCGTT
    GAAGGACAAA GTCCTCCAGG AACAGATGGA CTCAACCTGG ACACAGACCT TTTCATCGGC
    GGAGTGCTTG ATGACATGAG TGCAGACGTA AAGGAGAGGA CATCAGTTTC AGCAGGTCTG
    GTGGGCTGCA TCCGCATGCT GGACGTGAAC AACCTTGTCT ATAATCTGCA AGAGAATGGC
    GGCAGCGTTC TGTACGGTTC GGCCATTGGC GAGTGTGGCA ACAACCCATG CCTGCCAAAC
    CCCTGCAAGA ACGGAGCGTC CTGTCAGGTC AAAGAAGCCG AGATTTTCCA CTGCAAATGT
    GTCAACGGTT TCTCTGGCCC GACCTGCGCT GATGCCCACA ATCCCTGCGA CCCCAATAAA
    TGCCACCCGC CATCACGGTG CCAGGTCCTT CCAGAGGGAG GTTACAAATG CGAATGTCCG
    ATGGGACGTG AAGGCAGGCA CTGTGAAAAA GTGTCAGACA AGGGAGGCGC TTTCATTCCT
    TACTTCACTG GAGATTCTTT TCTGGAGCTC AAAGGCCTCC ATACGTATAA CCAGGATCTC
    AGGCAAAAAT TCAGCATGAC TATCGTTCTA CTGGCCAATG ACTCCAAAGG CATGATCTTC
    TACAATGGTC AGAAGACAGA CGGCAAAGGG GATTTCATCT CGCTCTCTCT TAATGACGGC
    ATCCTGGAGT TTAGATATGA CCTGGGCAAA GGGCCAGCTG TCATTAGGAG TAAAGAAAAA
    ATCAAGCTGA ACGAGTGGAA CACAGTGAAT TTGGAACGGG CCAGCAGAAA AGGAGAGATT
    AGCATTAACG GCAAAGACCC TGTCAGAGGA GAGGCGCCGA AATCACGCAA GAATCAGCAC
    ACGGATCTCA ATCTGAAGGA ATCGCTCTTT GTGGGTGGAG CTCCTGATTT CCGCAAAGTA
    GCCAGAGCTG CTGCGATCAA AGATGGCTTC AAAGGGGCCA TTCAGAAGAT TACTTTGATG
    GGAATTCCAA TCCTGAAAGC TGATAATGCC CTTTCGTCCA CTGACGTGTC CATGTATCCT
    AATCATCCCT GCTCCAAAGA CGTTTGTGAG AATGGAGGCC GCTGCAACCC CCAGCTGAAC
    AGCTATGAGT GCGCCTGCCG CCACGGATTC TCCGGACACC ACTGCCAGGA CACAATATTT
    GAGAAGTCCG CTGGGGACAC GGAGGCCATT GCGTTTGATG GTCATACATT CATCGAATAT
    CACAACGGAG TCACCAAAAG CCAACTGACC AATGAGATCC CAGACCCTGA GTCACTGGAG
    AACCCATCTG ACCAGAGCGA GAAAGCCCTG CTGGTGAATA AATTCGAGCT GAGCATCAGG
    ACGGAGGCTA CGCATGGTCT GATTTTGTGG AGCGGAAAGG GAGTTGAGCG CTCTGACTAC
    ATCGCCCTCG CCATTGTGGA TGGAAGAGTA CAGATGACCT ATGACCTCGG CTCCAAGCCT
    GTGGTGCTGC GCTCCACTGT ACGGGTCAAC ACCAACCACT GGATACGAAT TAAGGCCAGC
    AGAGCGCTCA GAGATGGCTC TCTGCAGGTT GGTAATGAGG CAGCTGTCAC TGGGTCTTCA
    CCCTTGGCTG CCACACAGCT GGACACAGAC GGGGCTCTCT GGTTGGGTGG ACTGGAGGAG
    CTGTCTGTGG CTCGACGGCT GCCCAAAGCT TACAGTACAG GCTTCATTGG CTGCGTCAAA
    GACGTGATAG TGGACGGCGT GGAGCTGCAT CTGGTGGAGG ACGCCTTAAA TACTCCCAAA
    ATATCACACT GTTCTGCCAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325511.1
    CDS266..6109
    Translation

    Target ORF information:

    RefSeq Version XM_021469836.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469836.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGGTAC AACAGTAGCT
    ACTACCACTC TTCCCACAGC TGAGGAGCGA TCGTCCTGTG ACAACACAGC GTTCGGCTGC
    TGTCCCGATG GGAAGACGGC CGCCGTCAAC TCAGAGGGCA CAAACTGCCC ATCCACCATG
    CGTTTCAGCG GCTTCCTGCA CCTGGATAAG GTTGAGGGTC AGGAGGTCTT CTACACGCCT
    GAGATGGAGG ACCCCAAATC GGAACTGTTT GGTGAAACGG CCCGAAGCAT CGAGAGCGCT
    ATGAATGAGT TGTTCCGCAA GTCTGATGTC CAGAAAGACT TCCAGAGTGT CCGTGTGAGG
    AATCTATCCC CCAGCAACTC CATAATAGCA CATTTCGAGG CTCACTTCGA CCCAGACACA
    AGGTTCAATG TGGGGGATAT TGAGGGAGCC CTTCTCAAAC AGCTTAAGGC GTCTAAAGAC
    ACTGGCATCG TTGTGAAGAA ACCAGAGGAA GAGAACATTC GCATCAACAA CTATGGTCTT
    TCAACTGTTC CTTTCTTTAC CACCACAACA ACCACTGTGG CATCTGTCAC GACTCCCACT
    ACCACCACCA CCACAATGTC TCCCAGTTCT GCTTATACCA CTCTCCGGCC CATCACTACT
    AGACGACCCT TCACAAGAAC CACCACGCCA GCACCAGCCA CCACCACTGC ACCACATGTG
    CCCAAGACCA CAGTTTCGCT TATGAACAGA CTAGGGCCCC ACGCTCACAG TCCATGTGAC
    TCTCAGCCCT GCCGACATGG AGGAACCTGT GAAGAAGACG ACAATGACTT CACCTGTATC
    TGTCCAGCAG GACGAGGAGG AGCCGTGTGT GAGAAAGTTA TAAAGTACTT CATACCATCC
    TTTGGTGGAA AGTCTTACAT GGCGTTCCAG ACCATGAAGG CCTACCATAC TGTACGCATT
    GCCATGGAGT TCAGGGCTTC GGAAATGACT GGGATATTGC TCTACAATGG GCAAAAAAGC
    AAAAAGGACT TTCTTTCTCT GACTCTAGTT GAAGGATATG TGGAGCTCAG GTTTAACACT
    GGTTCAGGGA CTGCTTCAGT TATCAGTAAA GTCCTGGTAA AGCCGGGTCG CTGGCATCAG
    CTTGTTGTAG TACGTAACAG GCGCAATGCC ATGCTGAGCG TGGACAATGA GCCCAGCGTT
    GAAGGACAAA GTCCTCCAGG AACAGATGGA CTCAACCTGG ACACAGACCT TTTCATCGGC
    GGAGTGCTTG ATGACATGAG TGCAGACGTA AAGGAGAGGA CATCAGTTTC AGCAGGTCTG
    GTGGGCTGCA TCCGCATGCT GGACGTGAAC AACCTTGTCT ATAATCTGCA AGAGAATGGC
    GGCAGCGTTC TGTACGGTTC GGCCATTGGC GAGTGTGGCA ACAACCCATG CCTGCCAAAC
    CCCTGCAAGA ACGGAGCGTC CTGTCAGGTC AAAGAAGCCG AGATTTTCCA CTGCAAATGT
    GTCAACGGTT TCTCTGGCCC GACCTGCGCT GATGCCCACA ATCCCTGCGA CCCCAATAAA
    TGCCACCCGC CATCACGGTG CCAGGTCCTT CCAGAGGGAG GTTACAAATG CGAATGTCCG
    ATGGGACGTG AAGGCAGGCA CTGTGAAAAA GTGTCAGACA AGGGAGGCGC TTTCATTCCT
    TACTTCACTG GAGATTCTTT TCTGGAGCTC AAAGGCCTCC ATACGTATAA CCAGGATCTC
    AGGCAAAAAT TCAGCATGAC TATCGTTCTA CTGGCCAATG ACTCCAAAGG CATGATCTTC
    TACAATGGTC AGAAGACAGA CGGCAAAGGG GATTTCATCT CGCTCTCTCT TAATGACGGC
    ATCCTGGAGT TTAGATATGA CCTGGGCAAA GGGCCAGCTG TCATTAGGAG TAAAGAAAAA
    ATCAAGCTGA ACGAGTGGAA CACAGTGAAT TTGGAACGGG CCAGCAGAAA AGGAGAGATT
    AGCATTAACG GCAAAGACCC TGTCAGAGGA GAGGCGCCGA AATCACGCAA GAATCAGCAC
    ACGGATCTCA ATCTGAAGGA ATCGCTCTTT GTGGGTGGAG CTCCTGATTT CCGCAAAGTA
    GCCAGAGCTG CTGCGATCAA AGATGGCTTC AAAGGGGCCA TTCAGAAGAT TACTTTGATG
    GGAATTCCAA TCCTGAAAGC TGATAATGCC CTTTCGTCCA CTGACGTGTC CATGTATCCT
    AATCATCCCT GCTCCAAAGA CGTTTGTGAG AATGGAGGCC GCTGCAACCC CCAGCTGAAC
    AGCTATGAGT GCGCCTGCCG CCACGGATTC TCCGGACACC ACTGCCAGGA CACAATATTT
    GAGAAGTCCG CTGGGGACAC GGAGGCCATT GCGTTTGATG GTCATACATT CATCGAATAT
    CACAACGGAG TCACCAAAAG CCAACTGACC AATGAGATCC CAGACCCTGA GTCACTGGAG
    AACCCATCTG ACCAGAGCGA GAAAGCCCTG CTGGTGAATA AATTCGAGCT GAGCATCAGG
    ACGGAGGCTA CGCATGGTCT GATTTTGTGG AGCGGAAAGG GAGTTGAGCG CTCTGACTAC
    ATCGCCCTCG CCATTGTGGA TGGAAGAGTA CAGATGACCT ATGACCTCGG CTCCAAGCCT
    GTGGTGCTGC GCTCCACTGT ACGGGTCAAC ACCAACCACT GGATACGAAT TAAGGCCAGC
    AGAGCGCTCA GAGATGGCTC TCTGCAGGTT GGTAATGAGG CAGCTGTCAC TGGGTCTTCA
    CCCTTGGCTG CCACACAGCT GGACACAGAC GGGGCTCTCT GGTTGGGTGG ACTGGAGGAG
    CTGTCTGTGG CTCGACGGCT GCCCAAAGCT TACAGTACAG GCTTCATTGG CTGCGTCAAA
    GACGTGATAG TGGACGGCGT GGAGCTGCAT CTGGTGGAGG ACGCCTTAAA TACTCCCAAA
    ATATCACACT GTTCTGCCAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44579
    Clone ID Related Accession (Same CDS sequence) XM_009296865.2
    Accession Version XM_009296865.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product agrin isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296865.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGACCAGTT GTCAGTACCC CACGCCGCCC CCAGAGCGGG ACCGCATGTA CCAGCACAAA 
    GTGTCGCTGG TCGTGCGATA CTTCATGATT CCTTGCAACA TCTGTCTCAT ACTGCTGGCC
    ACATCCACGC TCGGCTTTGC TGTACTCCTC TTCCTCAATA ACTGTAGGTC ACAGTTAGTG
    AAACATGACA TGCAGCATAA ACCTGTTCAC TTCACACCGG CACCTCCTCC TGATGGTTGC
    AGAGGGAAGT TGTGCGGATT TGGAGCGGTG TGTGAGAGAG ACCAAGCTGA CCCATCTAAA
    GGAGAGTGTG TGTGTAAGAA GATTGTGTGT ACATCTGTGG TGGCGCCAGT GTGCGGATCT
    GACTCCTCCA CCTATTCCAA TGAGTGTGAG CTGGAGAAGG CTCAGTGCAA TACACAGAGA
    CGCATCAAGG TGATGCGCAA AGGCCCCTGC GCCCTCAAGG ACCCGTGCAG TGAGGTAACC
    TGTAGCTTTG GCAGCACCTG CATCCAGTCA TCGGATGGTC TGTCCGCCAA GTGTATGTGT
    CCTCTGAGCT GTGAAAACGT CCCCAAGCAT GTGGTGTGTG GCAGTGACGG CCTGGACTAC
    CAAAGCGAAT GTGAACTCAA CATGAAGGCC TGCGCCACTC AGAAGAACAT CCGTGTGCAT
    CACCAGGGCC GCTGCGATCC TTGCAGTGAC ACTCTGAACA GCTTAAGCAC AGCCTGTCGA
    GTCAACCCAA AGACCTACCA GCAGATTACC TTTGCCCCGG CTGAGTCTTG TCCGCCTGAC
    GAAATGCCCA TCTGTGCCAG CGACGGACAC ACCTACGAGA GCATGTGTCA GATGGAGCGC
    ACTGCCCTGA AGAACAACCT GGAGCTCAAA AAGGTCTCTT TAGGCAGCTG CAAGGAAAAA
    GGGGGCTGCC CGAATGGTTG TAAGTTCAAC GCCATATGTT TGCTGGATAA TGGAGAGTTT
    CGGTGCTCCT GCGATCCCAT ACAATGCGAC GGCACGTACA AGCCCCTGTG CGGAAAGGAT
    GGCAAGACAT ACCCCAACGA CTGCGAGAGG AAACTGCAGG AATGCCGGAC CCAAAAAGAC
    ATCCCTGTCA AACAACAAGG GCCCTGTGAT CTTAACCTGG AGAGCCCCTG CCTGAAGAAG
    ACCTGCGAGT TTGGAGCCGT GTGCGTGGTG AAGAACAGCG AAGCAGTGTG TGAATGCTCA
    GACGCCTGTC CGCAGGACCA AGACCCTGTT TGCGGCAGCG ATGGGCACAC GTACAGCAGC
    TCATGTCAAA TGAAGGCCAT GGGATGCGCT CTTCAGAAAC AGATACAGAT GCAACACAAA
    GGACCATGTG CAGATGAGTC CTGCACCAAC TGCTCGTTTG GCGCGATCTG TGATGCCCAG
    ACTGGCCGGT GCGTGTGCCC TAAAGGCTGT TTGGAAACAC GGCAGCCCGT ATGCGGCAGC
    GACGGCACCA CGTATGACAA CGAGTGCAAG CTCAACGTCC AAGCCTGCAC TAAACAGCTG
    GATCTCAAGG TGGTGGCCCA TGGAGAATGC AAAACCTGTG GCAGCACTGT GTGTAGCTGG
    GGGTCGCAGT GCGTGAACAA CAAGTGTGAG TGTCAGCAGT GCACTGGTCA GCCGGTGAAA
    CCCGTGTGTG GCAGCGACGG CAACACATAC AACAACGACT GCGAGCTGAG ACTGGCCTCC
    TGTCAGAAAC AGAGGAAGAT AGAGGTGGCC AAGCCTGGCG TCTGCGATGA AGACTGTGGT
    TCAGGAGGCT CCGGCTCGGG ATCTGAGACT TGTGAACAGG ACCGATGTCG CCGATATGGA
    GGAACGTGGG ACGATGACAT GGAGGACGAG CGATGTGTTT GTGAACTAAA CTGTCAGATT
    GTGCTGCACA GCCCGGTGTG CGGCTCTGAT GGAAACACGT ACTCCAGCGA CTGTGAGCTC
    AAGAAGACCA GGTGCGAAAA GCAAATAGAC CTGAGTGTCC AAAGCCAAGG GCCCTGTCCA
    ACTATCGTAT CACCACCTCC AGAGCTTGTT GCCTCCCAGC ACTGTAGCCA AACAGTTTAT
    GGATGTTGTG AAGATAACAA GACCGCTGCC CTGGGTGTTG GACTGGCTGG ATGTCCTAGT
    ATGTGTCAGT GCAATCATTA CGGCTCATAC GGAGGCACAT GTGACCCCTC GACTGGCCAG
    TGTTCCTGTA AACCTGGTGT CGGTGGACTG AAGTGCGACC GCTGTGAGCC CGGATTTTGG
    AACTTCCGTG GCATTGTCAC AGAAAACATG AGCGGCTGCA CACCGTGCAA CTGTGATGGA
    GTGGGTTCGG TTCGTGATGA CTGCGAGCAG ATGTCAGGCC TGTGCTCCTG TAAGCCTGGA
    GTTAAGGGCA TGAAATGCAA CGTGTGCCCT GATGGCAGCA AGATGGGAGT CAACGGCTGT
    GACAAAGGAC CTAAGGCTCC CAAGTCCTGT GCTGAGCTTG TGTGCCACTT TGGGTCTTCA
    TGCGTGGAGG TGAATGGACA GGCTCACTGT GAGTGTCCTT CACCAGATTG TGATGAGAAA
    AACAAGACCA AGGTATGTGG TTCTGATGGG GTCACCTATG CAGACCGGTG CCAGCTGAAG
    ACCATCGCAT GCCGACAAGA TAAAGAGATC AAGGTGGAGC ATTTAGGCCA GTGTAAAGAA
    TCCATTGAGC TGACGGCCAA ACCCACCCCC TTCCCAACCA CCCACCGCAC CACCCCTCCC
    ACCGCTCACG TCAAGGTCAC CAAACAGCGA CATGGCCACC GCACCACCAC TCCGGACAGC
    TATGTGGTGG AGGCAATGCC TCCACCGAGG TTGGATGGAG CGCGAAAGTT TTCCAGCCCT
    TTCACCACCC ACCGTCCCAC TACACCCTAC AAACCACTCC GCACCTCATC CCCACCTCTG
    GACTTTGACG AGTCGGGCAG TGGAGAGCCC AGTGGGGATG ATGAGATGGT GAGCGGAATG
    GAGGAGAGCG GAGAAGAGCC ACTTGGTACA ACAGTAGCTA CTACCACTCT TCCCACAGCT
    GAGGAGCGAT CGTCCTGTGA CAACACAGCG TTCGGCTGCT GTCCCGATGG GAAGACGGCC
    GCCGTCAACT CAGAGGGCAC AAACTGCCCA TCCACCATGC GTTTCAGCGG CTTCCTGCAC
    CTGGATAAGG TTGAGGGTCA GGAGGTCTTC TACACGCCTG AGATGGAGGA CCCCAAATCG
    GAACTGTTTG GTGAAACGGC CCGAAGCATC GAGAGCGCTA TGAATGAGTT GTTCCGCAAG
    TCTGATGTCC AGAAAGACTT CCAGAGTGTC CGTGTGAGGA ATCTATCCCC CAGCAACTCC
    ATAATAGCAC ATTTCGAGGC TCACTTCGAC CCAGACACAA GGTTCAATGT GGGGGATATT
    GAGGGAGCCC TTCTCAAACA GCTTAAGGCG TCTAAAGACA CTGGCATCGT TGTGAAGAAA
    CCAGAGGAAG AGAACATTCG CATCAACAAC TATGGTCTTT CAACTGTTCC TTTCTTTACC
    ACCACAACAA CCACTGTGGC ATCTGTCACG ACTCCCACTA CCACCACCAC CACAATGTCT
    CCCAGTTCTG CTTATACCAC TCTCCGGCCC ATCACTACTA GACGACCCTT CACAAGAACC
    ACCACGCCAG CACCAGCCAC CACCACTGCA CCACATGTGC CCAAGACCAC AGTTTCGCTT
    ATGAACAGAC TAGGGCCCCA CGCTCACAGT CCATGTGACT CTCAGCCCTG CCGACATGGA
    GGAACCTGTG AAGAAGACGA CAATGACTTC ACCTGTATCT GTCCAGCAGG ACGAGGAGGA
    GCCGTGTGTG AGAAAGTTAT AAAGTACTTC ATACCATCCT TTGGTGGAAA GTCTTACATG
    GCGTTCCAGA CCATGAAGGC CTACCATACT GTACGCATTG CCATGGAGTT CAGGGCTTCG
    GAAATGACTG GGATATTGCT CTACAATGGG CAAAAAAGCA AAAAGGACTT TCTTTCTCTG
    ACTCTAGTTG AAGGATATGT GGAGCTCAGG TTTAACACTG GTTCAGGGAC TGCTTCAGTT
    ATCAGTAAAG TCCTGGTAAA GCCGGGTCGC TGGCATCAGC TTGTTGTAGT ACGTAACAGG
    CGCAATGCCA TGCTGAGCGT GGACAATGAG CCCAGCGTTG AAGGACAAAG TCCTCCAGGA
    ACAGATGGAC TCAACCTGGA CACAGACCTT TTCATCGGCG GAGTGCTTGA TGACATGAGT
    GCAGACGTAA AGGAGAGGAC ATCAGTTTCA GCAGGTCTGG TGGGCTGCAT CCGCATGCTG
    GACGTGAACA ACCTTGTCTA TAATCTGCAA GAGAATGGCG GCAGCGTTCT GTACGGTTCG
    GCCATTGGCG AGTGTGGCAA CAACCCATGC CTGCCAAACC CCTGCAAGAA CGGAGCGTCC
    TGTCAGGTCA AAGAAGCCGA GATTTTCCAC TGCAAATGTG TCAACGGTTT CTCTGGCCCG
    ACCTGCGCTG ATGCCCACAA TCCCTGCGAC CCCAATAAAT GCCACCCGCC ATCACGGTGC
    CAGGTCCTTC CAGAGGGAGG TTACAAATGC GAATGTCCGA TGGGACGTGA AGGCAGGCAC
    TGTGAAAAAG TGTCAGACAA GGGAGGCGCT TTCATTCCTT ACTTCACTGG AGATTCTTTT
    CTGGAGCTCA AAGGCCTCCA TACGTATAAC CAGGATCTCA GGCAAAAATT CAGCATGACT
    ATCGTTCTAC TGGCCAATGA CTCCAAAGGC ATGATCTTCT ACAATGGTCA GAAGACAGAC
    GGCAAAGGGG ATTTCATCTC GCTCTCTCTT AATGACGGCA TCCTGGAGTT TAGATATGAC
    CTGGGCAAAG GGCCAGCTGT CATTAGGAGT AAAGAAAAAA TCAAGCTGAA CGAGTGGAAC
    ACAGTGAATT TGGAACGGGC CAGCAGAAAA GGAGAGATTA GCATTAACGG CAAAGACCCT
    GTCAGAGGAG AGGCGCCGAA ATCACGCAAG AATCAGCACA CGGATCTCAA TCTGAAGGAA
    TCGCTCTTTG TGGGTGGAGC TCCTGATTTC CGCAAAGTAG CCAGAGCTGC TGCGATCAAA
    GATGGCTTCA AAGGGGCCAT TCAGAAGATT ACTTTGATGG GAATTCCAAT CCTGAAAGCT
    GATAATGCCC TTTCGTCCAC TGACGTGTCC ATGTATCCTA ATCATCCCTG CTCCAAAGAC
    GTTTGTGAGA ATGGAGGCCG CTGCAACCCC CAGCTGAACA GCTATGAGTG CGCCTGCCGC
    CACGGATTCT CCGGACACCA CTGCCAGGAC ACAATATTTG AGAAGTCCGC TGGGGACACG
    GAGGCCATTG CGTTTGATGG TCATACATTC ATCGAATATC ACAACGGAGT CACCAAAAGC
    CCTGAGTCAC TGGAGAACCC ATCTGACCAG AGCGAGAAAG CCCTGCTGGT GAATAAATTC
    GAGCTGAGCA TCAGGACGGA GGCTACGCAT GGTCTGATTT TGTGGAGCGG AAAGGGAGTT
    GAGCGCTCTG ACTACATCGC CCTCGCCATT GTGGATGGAA GAGTACAGAT GACCTATGAC
    CTCGGCTCCA AGCCTGTGGT GCTGCGCTCC ACTGTACGGG TCAACACCAA CCACTGGATA
    CGAATTAAGG CCAGCAGAGC GCTCAGAGAT GGCTCTCTGC AGGTTGGTAA TGAGGCAGCT
    GTCACTGGGT CTTCACCCTT GGCTGCCACA CAGCTGGACA CAGACGGGGC TCTCTGGTTG
    GGTGGACTGG AGGAGCTGTC TGTGGCTCGA CGGCTGCCCA AAGCTTACAG TACAGGCTTC
    ATTGGCTGCG TCAAAGACGT GATAGTGGAC GGCGTGGAGC TGCATCTGGT GGAGGACGCC
    TTAAATACTC CCAAAATATC ACACTGTTCT GCCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295140.1
    CDS1636..7494
    Translation

    Target ORF information:

    RefSeq Version XM_009296865.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296865.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGACCAGTT GTCAGTACCC CACGCCGCCC CCAGAGCGGG ACCGCATGTA CCAGCACAAA 
    GTGTCGCTGG TCGTGCGATA CTTCATGATT CCTTGCAACA TCTGTCTCAT ACTGCTGGCC
    ACATCCACGC TCGGCTTTGC TGTACTCCTC TTCCTCAATA ACTGTAGGTC ACAGTTAGTG
    AAACATGACA TGCAGCATAA ACCTGTTCAC TTCACACCGG CACCTCCTCC TGATGGTTGC
    AGAGGGAAGT TGTGCGGATT TGGAGCGGTG TGTGAGAGAG ACCAAGCTGA CCCATCTAAA
    GGAGAGTGTG TGTGTAAGAA GATTGTGTGT ACATCTGTGG TGGCGCCAGT GTGCGGATCT
    GACTCCTCCA CCTATTCCAA TGAGTGTGAG CTGGAGAAGG CTCAGTGCAA TACACAGAGA
    CGCATCAAGG TGATGCGCAA AGGCCCCTGC GCCCTCAAGG ACCCGTGCAG TGAGGTAACC
    TGTAGCTTTG GCAGCACCTG CATCCAGTCA TCGGATGGTC TGTCCGCCAA GTGTATGTGT
    CCTCTGAGCT GTGAAAACGT CCCCAAGCAT GTGGTGTGTG GCAGTGACGG CCTGGACTAC
    CAAAGCGAAT GTGAACTCAA CATGAAGGCC TGCGCCACTC AGAAGAACAT CCGTGTGCAT
    CACCAGGGCC GCTGCGATCC TTGCAGTGAC ACTCTGAACA GCTTAAGCAC AGCCTGTCGA
    GTCAACCCAA AGACCTACCA GCAGATTACC TTTGCCCCGG CTGAGTCTTG TCCGCCTGAC
    GAAATGCCCA TCTGTGCCAG CGACGGACAC ACCTACGAGA GCATGTGTCA GATGGAGCGC
    ACTGCCCTGA AGAACAACCT GGAGCTCAAA AAGGTCTCTT TAGGCAGCTG CAAGGAAAAA
    GGGGGCTGCC CGAATGGTTG TAAGTTCAAC GCCATATGTT TGCTGGATAA TGGAGAGTTT
    CGGTGCTCCT GCGATCCCAT ACAATGCGAC GGCACGTACA AGCCCCTGTG CGGAAAGGAT
    GGCAAGACAT ACCCCAACGA CTGCGAGAGG AAACTGCAGG AATGCCGGAC CCAAAAAGAC
    ATCCCTGTCA AACAACAAGG GCCCTGTGAT CTTAACCTGG AGAGCCCCTG CCTGAAGAAG
    ACCTGCGAGT TTGGAGCCGT GTGCGTGGTG AAGAACAGCG AAGCAGTGTG TGAATGCTCA
    GACGCCTGTC CGCAGGACCA AGACCCTGTT TGCGGCAGCG ATGGGCACAC GTACAGCAGC
    TCATGTCAAA TGAAGGCCAT GGGATGCGCT CTTCAGAAAC AGATACAGAT GCAACACAAA
    GGACCATGTG CAGATGAGTC CTGCACCAAC TGCTCGTTTG GCGCGATCTG TGATGCCCAG
    ACTGGCCGGT GCGTGTGCCC TAAAGGCTGT TTGGAAACAC GGCAGCCCGT ATGCGGCAGC
    GACGGCACCA CGTATGACAA CGAGTGCAAG CTCAACGTCC AAGCCTGCAC TAAACAGCTG
    GATCTCAAGG TGGTGGCCCA TGGAGAATGC AAAACCTGTG GCAGCACTGT GTGTAGCTGG
    GGGTCGCAGT GCGTGAACAA CAAGTGTGAG TGTCAGCAGT GCACTGGTCA GCCGGTGAAA
    CCCGTGTGTG GCAGCGACGG CAACACATAC AACAACGACT GCGAGCTGAG ACTGGCCTCC
    TGTCAGAAAC AGAGGAAGAT AGAGGTGGCC AAGCCTGGCG TCTGCGATGA AGACTGTGGT
    TCAGGAGGCT CCGGCTCGGG ATCTGAGACT TGTGAACAGG ACCGATGTCG CCGATATGGA
    GGAACGTGGG ACGATGACAT GGAGGACGAG CGATGTGTTT GTGAACTAAA CTGTCAGATT
    GTGCTGCACA GCCCGGTGTG CGGCTCTGAT GGAAACACGT ACTCCAGCGA CTGTGAGCTC
    AAGAAGACCA GGTGCGAAAA GCAAATAGAC CTGAGTGTCC AAAGCCAAGG GCCCTGTCCA
    ACTATCGTAT CACCACCTCC AGAGCTTGTT GCCTCCCAGC ACTGTAGCCA AACAGTTTAT
    GGATGTTGTG AAGATAACAA GACCGCTGCC CTGGGTGTTG GACTGGCTGG ATGTCCTAGT
    ATGTGTCAGT GCAATCATTA CGGCTCATAC GGAGGCACAT GTGACCCCTC GACTGGCCAG
    TGTTCCTGTA AACCTGGTGT CGGTGGACTG AAGTGCGACC GCTGTGAGCC CGGATTTTGG
    AACTTCCGTG GCATTGTCAC AGAAAACATG AGCGGCTGCA CACCGTGCAA CTGTGATGGA
    GTGGGTTCGG TTCGTGATGA CTGCGAGCAG ATGTCAGGCC TGTGCTCCTG TAAGCCTGGA
    GTTAAGGGCA TGAAATGCAA CGTGTGCCCT GATGGCAGCA AGATGGGAGT CAACGGCTGT
    GACAAAGGAC CTAAGGCTCC CAAGTCCTGT GCTGAGCTTG TGTGCCACTT TGGGTCTTCA
    TGCGTGGAGG TGAATGGACA GGCTCACTGT GAGTGTCCTT CACCAGATTG TGATGAGAAA
    AACAAGACCA AGGTATGTGG TTCTGATGGG GTCACCTATG CAGACCGGTG CCAGCTGAAG
    ACCATCGCAT GCCGACAAGA TAAAGAGATC AAGGTGGAGC ATTTAGGCCA GTGTAAAGAA
    TCCATTGAGC TGACGGCCAA ACCCACCCCC TTCCCAACCA CCCACCGCAC CACCCCTCCC
    ACCGCTCACG TCAAGGTCAC CAAACAGCGA CATGGCCACC GCACCACCAC TCCGGACAGC
    TATGTGGTGG AGGCAATGCC TCCACCGAGG TTGGATGGAG CGCGAAAGTT TTCCAGCCCT
    TTCACCACCC ACCGTCCCAC TACACCCTAC AAACCACTCC GCACCTCATC CCCACCTCTG
    GACTTTGACG AGTCGGGCAG TGGAGAGCCC AGTGGGGATG ATGAGATGGT GAGCGGAATG
    GAGGAGAGCG GAGAAGAGCC ACTTGGTACA ACAGTAGCTA CTACCACTCT TCCCACAGCT
    GAGGAGCGAT CGTCCTGTGA CAACACAGCG TTCGGCTGCT GTCCCGATGG GAAGACGGCC
    GCCGTCAACT CAGAGGGCAC AAACTGCCCA TCCACCATGC GTTTCAGCGG CTTCCTGCAC
    CTGGATAAGG TTGAGGGTCA GGAGGTCTTC TACACGCCTG AGATGGAGGA CCCCAAATCG
    GAACTGTTTG GTGAAACGGC CCGAAGCATC GAGAGCGCTA TGAATGAGTT GTTCCGCAAG
    TCTGATGTCC AGAAAGACTT CCAGAGTGTC CGTGTGAGGA ATCTATCCCC CAGCAACTCC
    ATAATAGCAC ATTTCGAGGC TCACTTCGAC CCAGACACAA GGTTCAATGT GGGGGATATT
    GAGGGAGCCC TTCTCAAACA GCTTAAGGCG TCTAAAGACA CTGGCATCGT TGTGAAGAAA
    CCAGAGGAAG AGAACATTCG CATCAACAAC TATGGTCTTT CAACTGTTCC TTTCTTTACC
    ACCACAACAA CCACTGTGGC ATCTGTCACG ACTCCCACTA CCACCACCAC CACAATGTCT
    CCCAGTTCTG CTTATACCAC TCTCCGGCCC ATCACTACTA GACGACCCTT CACAAGAACC
    ACCACGCCAG CACCAGCCAC CACCACTGCA CCACATGTGC CCAAGACCAC AGTTTCGCTT
    ATGAACAGAC TAGGGCCCCA CGCTCACAGT CCATGTGACT CTCAGCCCTG CCGACATGGA
    GGAACCTGTG AAGAAGACGA CAATGACTTC ACCTGTATCT GTCCAGCAGG ACGAGGAGGA
    GCCGTGTGTG AGAAAGTTAT AAAGTACTTC ATACCATCCT TTGGTGGAAA GTCTTACATG
    GCGTTCCAGA CCATGAAGGC CTACCATACT GTACGCATTG CCATGGAGTT CAGGGCTTCG
    GAAATGACTG GGATATTGCT CTACAATGGG CAAAAAAGCA AAAAGGACTT TCTTTCTCTG
    ACTCTAGTTG AAGGATATGT GGAGCTCAGG TTTAACACTG GTTCAGGGAC TGCTTCAGTT
    ATCAGTAAAG TCCTGGTAAA GCCGGGTCGC TGGCATCAGC TTGTTGTAGT ACGTAACAGG
    CGCAATGCCA TGCTGAGCGT GGACAATGAG CCCAGCGTTG AAGGACAAAG TCCTCCAGGA
    ACAGATGGAC TCAACCTGGA CACAGACCTT TTCATCGGCG GAGTGCTTGA TGACATGAGT
    GCAGACGTAA AGGAGAGGAC ATCAGTTTCA GCAGGTCTGG TGGGCTGCAT CCGCATGCTG
    GACGTGAACA ACCTTGTCTA TAATCTGCAA GAGAATGGCG GCAGCGTTCT GTACGGTTCG
    GCCATTGGCG AGTGTGGCAA CAACCCATGC CTGCCAAACC CCTGCAAGAA CGGAGCGTCC
    TGTCAGGTCA AAGAAGCCGA GATTTTCCAC TGCAAATGTG TCAACGGTTT CTCTGGCCCG
    ACCTGCGCTG ATGCCCACAA TCCCTGCGAC CCCAATAAAT GCCACCCGCC ATCACGGTGC
    CAGGTCCTTC CAGAGGGAGG TTACAAATGC GAATGTCCGA TGGGACGTGA AGGCAGGCAC
    TGTGAAAAAG TGTCAGACAA GGGAGGCGCT TTCATTCCTT ACTTCACTGG AGATTCTTTT
    CTGGAGCTCA AAGGCCTCCA TACGTATAAC CAGGATCTCA GGCAAAAATT CAGCATGACT
    ATCGTTCTAC TGGCCAATGA CTCCAAAGGC ATGATCTTCT ACAATGGTCA GAAGACAGAC
    GGCAAAGGGG ATTTCATCTC GCTCTCTCTT AATGACGGCA TCCTGGAGTT TAGATATGAC
    CTGGGCAAAG GGCCAGCTGT CATTAGGAGT AAAGAAAAAA TCAAGCTGAA CGAGTGGAAC
    ACAGTGAATT TGGAACGGGC CAGCAGAAAA GGAGAGATTA GCATTAACGG CAAAGACCCT
    GTCAGAGGAG AGGCGCCGAA ATCACGCAAG AATCAGCACA CGGATCTCAA TCTGAAGGAA
    TCGCTCTTTG TGGGTGGAGC TCCTGATTTC CGCAAAGTAG CCAGAGCTGC TGCGATCAAA
    GATGGCTTCA AAGGGGCCAT TCAGAAGATT ACTTTGATGG GAATTCCAAT CCTGAAAGCT
    GATAATGCCC TTTCGTCCAC TGACGTGTCC ATGTATCCTA ATCATCCCTG CTCCAAAGAC
    GTTTGTGAGA ATGGAGGCCG CTGCAACCCC CAGCTGAACA GCTATGAGTG CGCCTGCCGC
    CACGGATTCT CCGGACACCA CTGCCAGGAC ACAATATTTG AGAAGTCCGC TGGGGACACG
    GAGGCCATTG CGTTTGATGG TCATACATTC ATCGAATATC ACAACGGAGT CACCAAAAGC
    CCTGAGTCAC TGGAGAACCC ATCTGACCAG AGCGAGAAAG CCCTGCTGGT GAATAAATTC
    GAGCTGAGCA TCAGGACGGA GGCTACGCAT GGTCTGATTT TGTGGAGCGG AAAGGGAGTT
    GAGCGCTCTG ACTACATCGC CCTCGCCATT GTGGATGGAA GAGTACAGAT GACCTATGAC
    CTCGGCTCCA AGCCTGTGGT GCTGCGCTCC ACTGTACGGG TCAACACCAA CCACTGGATA
    CGAATTAAGG CCAGCAGAGC GCTCAGAGAT GGCTCTCTGC AGGTTGGTAA TGAGGCAGCT
    GTCACTGGGT CTTCACCCTT GGCTGCCACA CAGCTGGACA CAGACGGGGC TCTCTGGTTG
    GGTGGACTGG AGGAGCTGTC TGTGGCTCGA CGGCTGCCCA AAGCTTACAG TACAGGCTTC
    ATTGGCTGCG TCAAAGACGT GATAGTGGAC GGCGTGGAGC TGCATCTGGT GGAGGACGCC
    TTAAATACTC CCAAAATATC ACACTGTTCT GCCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44580
    Clone ID Related Accession (Same CDS sequence) XM_009296866.2
    Accession Version XM_009296866.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5826bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product agrin isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296866.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGACCAGTT GTCAGTACCC CACGCCGCCC CCAGAGCGGG ACCGCATGTA CCAGCACAAA 
    GTGTCGCTGG TCGTGCGATA CTTCATGATT CCTTGCAACA TCTGTCTCAT ACTGCTGGCC
    ACATCCACGC TCGGCTTTGC TGTACTCCTC TTCCTCAATA ACTATAAACC TGTTCACTTC
    ACACCGGCAC CTCCTCCTGA TGGTTGCAGA GGGAAGTTGT GCGGATTTGG AGCGGTGTGT
    GAGAGAGACC AAGCTGACCC ATCTAAAGGA GAGTGTGTGT GTAAGAAGAT TGTGTGTACA
    TCTGTGGTGG CGCCAGTGTG CGGATCTGAC TCCTCCACCT ATTCCAATGA GTGTGAGCTG
    GAGAAGGCTC AGTGCAATAC ACAGAGACGC ATCAAGGTGA TGCGCAAAGG CCCCTGCGCC
    CTCAAGGACC CGTGCAGTGA GGTAACCTGT AGCTTTGGCA GCACCTGCAT CCAGTCATCG
    GATGGTCTGT CCGCCAAGTG TATGTGTCCT CTGAGCTGTG AAAACGTCCC CAAGCATGTG
    GTGTGTGGCA GTGACGGCCT GGACTACCAA AGCGAATGTG AACTCAACAT GAAGGCCTGC
    GCCACTCAGA AGAACATCCG TGTGCATCAC CAGGGCCGCT GCGATCCTTG CAGTGACACT
    CTGAACAGCT TAAGCACAGC CTGTCGAGTC AACCCAAAGA CCTACCAGCA GATTACCTTT
    GCCCCGGCTG AGTCTTGTCC GCCTGACGAA ATGCCCATCT GTGCCAGCGA CGGACACACC
    TACGAGAGCA TGTGTCAGAT GGAGCGCACT GCCCTGAAGA ACAACCTGGA GCTCAAAAAG
    GTCTCTTTAG GCAGCTGCAA GGAAAAAGGG GGCTGCCCGA ATGGTTGTAA GTTCAACGCC
    ATATGTTTGC TGGATAATGG AGAGTTTCGG TGCTCCTGCG ATCCCATACA ATGCGACGGC
    ACGTACAAGC CCCTGTGCGG AAAGGATGGC AAGACATACC CCAACGACTG CGAGAGGAAA
    CTGCAGGAAT GCCGGACCCA AAAAGACATC CCTGTCAAAC AACAAGGGCC CTGTGATCTT
    AACCTGGAGA GCCCCTGCCT GAAGAAGACC TGCGAGTTTG GAGCCGTGTG CGTGGTGAAG
    AACAGCGAAG CAGTGTGTGA ATGCTCAGAC GCCTGTCCGC AGGACCAAGA CCCTGTTTGC
    GGCAGCGATG GGCACACGTA CAGCAGCTCA TGTCAAATGA AGGCCATGGG ATGCGCTCTT
    CAGAAACAGA TACAGATGCA ACACAAAGGA CCATGTGCAG ATGAGTCCTG CACCAACTGC
    TCGTTTGGCG CGATCTGTGA TGCCCAGACT GGCCGGTGCG TGTGCCCTAA AGGCTGTTTG
    GAAACACGGC AGCCCGTATG CGGCAGCGAC GGCACCACGT ATGACAACGA GTGCAAGCTC
    AACGTCCAAG CCTGCACTAA ACAGCTGGAT CTCAAGGTGG TGGCCCATGG AGAATGCAAA
    ACCTGTGGCA GCACTGTGTG TAGCTGGGGG TCGCAGTGCG TGAACAACAA GTGTGAGTGT
    CAGCAGTGCA CTGGTCAGCC GGTGAAACCC GTGTGTGGCA GCGACGGCAA CACATACAAC
    AACGACTGCG AGCTGAGACT GGCCTCCTGT CAGAAACAGA GGAAGATAGA GGTGGCCAAG
    CCTGGCGTCT GCGATGAAGA CTGTGGTTCA GGAGGCTCCG GCTCGGGATC TGAGACTTGT
    GAACAGGACC GATGTCGCCG ATATGGAGGA ACGTGGGACG ATGACATGGA GGACGAGCGA
    TGTGTTTGTG AACTAAACTG TCAGATTGTG CTGCACAGCC CGGTGTGCGG CTCTGATGGA
    AACACGTACT CCAGCGACTG TGAGCTCAAG AAGACCAGGT GCGAAAAGCA AATAGACCTG
    AGTGTCCAAA GCCAAGGGCC CTGTCCAACT ATCGTATCAC CACCTCCAGA GCTTGTTGCC
    TCCCAGCACT GTAGCCAAAC AGTTTATGGA TGTTGTGAAG ATAACAAGAC CGCTGCCCTG
    GGTGTTGGAC TGGCTGGATG TCCTAGTATG TGTCAGTGCA ATCATTACGG CTCATACGGA
    GGCACATGTG ACCCCTCGAC TGGCCAGTGT TCCTGTAAAC CTGGTGTCGG TGGACTGAAG
    TGCGACCGCT GTGAGCCCGG ATTTTGGAAC TTCCGTGGCA TTGTCACAGA AAACATGAGC
    GGCTGCACAC CGTGCAACTG TGATGGAGTG GGTTCGGTTC GTGATGACTG CGAGCAGATG
    TCAGGCCTGT GCTCCTGTAA GCCTGGAGTT AAGGGCATGA AATGCAACGT GTGCCCTGAT
    GGCAGCAAGA TGGGAGTCAA CGGCTGTGAC AAAGGACCTA AGGCTCCCAA GTCCTGTGCT
    GAGCTTGTGT GCCACTTTGG GTCTTCATGC GTGGAGGTGA ATGGACAGGC TCACTGTGAG
    TGTCCTTCAC CAGATTGTGA TGAGAAAAAC AAGACCAAGG TATGTGGTTC TGATGGGGTC
    ACCTATGCAG ACCGGTGCCA GCTGAAGACC ATCGCATGCC GACAAGATAA AGAGATCAAG
    GTGGAGCATT TAGGCCAGTG TAAAGAATCC ATTGAGCTGA CGGCCAAACC CACCCCCTTC
    CCAACCACCC ACCGCACCAC CCCTCCCACC GCTCACGTCA AGGTCACCAA ACAGCGACAT
    GGCCACCGCA CCACCACTCC GGACAGCTAT GTGGTGGAGG CAATGCCTCC ACCGAGGTTG
    GATGGAGCGC GAAAGTTTTC CAGCCCTTTC ACCACCCACC GTCCCACTAC ACCCTACAAA
    CCACTCCGCA CCTCATCCCC ACCTCTGGAC TTTGACGAGT CGGGCAGTGG AGAGCCCAGT
    GGGGATGATG AGATGGTGAG CGGAATGGAG GAGAGCGGAG AAGAGCCACT TGGTACAACA
    GTAGCTACTA CCACTCTTCC CACAGCTGAG GAGCGATCGT CCTGTGACAA CACAGCGTTC
    GGCTGCTGTC CCGATGGGAA GACGGCCGCC GTCAACTCAG AGGGCACAAA CTGCCCATCC
    ACCATGCGTT TCAGCGGCTT CCTGCACCTG GATAAGGTTG AGGGTCAGGA GGTCTTCTAC
    ACGCCTGAGA TGGAGGACCC CAAATCGGAA CTGTTTGGTG AAACGGCCCG AAGCATCGAG
    AGCGCTATGA ATGAGTTGTT CCGCAAGTCT GATGTCCAGA AAGACTTCCA GAGTGTCCGT
    GTGAGGAATC TATCCCCCAG CAACTCCATA ATAGCACATT TCGAGGCTCA CTTCGACCCA
    GACACAAGGT TCAATGTGGG GGATATTGAG GGAGCCCTTC TCAAACAGCT TAAGGCGTCT
    AAAGACACTG GCATCGTTGT GAAGAAACCA GAGGAAGAGA ACATTCGCAT CAACAACTAT
    GGTCTTTCAA CTGTTCCTTT CTTTACCACC ACAACAACCA CTGTGGCATC TGTCACGACT
    CCCACTACCA CCACCACCAC AATGTCTCCC AGTTCTGCTT ATACCACTCT CCGGCCCATC
    ACTACTAGAC GACCCTTCAC AAGAACCACC ACGCCAGCAC CAGCCACCAC CACTGCACCA
    CATGTGCCCA AGACCACAGT TTCGCTTATG AACAGACTAG GGCCCCACGC TCACAGTCCA
    TGTGACTCTC AGCCCTGCCG ACATGGAGGA ACCTGTGAAG AAGACGACAA TGACTTCACC
    TGTATCTGTC CAGCAGGACG AGGAGGAGCC GTGTGTGAGA AAGTTATAAA GTACTTCATA
    CCATCCTTTG GTGGAAAGTC TTACATGGCG TTCCAGACCA TGAAGGCCTA CCATACTGTA
    CGCATTGCCA TGGAGTTCAG GGCTTCGGAA ATGACTGGGA TATTGCTCTA CAATGGGCAA
    AAAAGCAAAA AGGACTTTCT TTCTCTGACT CTAGTTGAAG GATATGTGGA GCTCAGGTTT
    AACACTGGTT CAGGGACTGC TTCAGTTATC AGTAAAGTCC TGGTAAAGCC GGGTCGCTGG
    CATCAGCTTG TTGTAGTACG TAACAGGCGC AATGCCATGC TGAGCGTGGA CAATGAGCCC
    AGCGTTGAAG GACAAAGTCC TCCAGGAACA GATGGACTCA ACCTGGACAC AGACCTTTTC
    ATCGGCGGAG TGCTTGATGA CATGAGTGCA GACGTAAAGG AGAGGACATC AGTTTCAGCA
    GGTCTGGTGG GCTGCATCCG CATGCTGGAC GTGAACAACC TTGTCTATAA TCTGCAAGAG
    AATGGCGGCA GCGTTCTGTA CGGTTCGGCC ATTGGCGAGT GTGGCAACAA CCCATGCCTG
    CCAAACCCCT GCAAGAACGG AGCGTCCTGT CAGGTCAAAG AAGCCGAGAT TTTCCACTGC
    AAATGTGTCA ACGGTTTCTC TGGCCCGACC TGCGCTGATG CCCACAATCC CTGCGACCCC
    AATAAATGCC ACCCGCCATC ACGGTGCCAG GTCCTTCCAG AGGGAGGTTA CAAATGCGAA
    TGTCCGATGG GACGTGAAGG CAGGCACTGT GAAAAAGTGT CAGACAAGGG AGGCGCTTTC
    ATTCCTTACT TCACTGGAGA TTCTTTTCTG GAGCTCAAAG GCCTCCATAC GTATAACCAG
    GATCTCAGGC AAAAATTCAG CATGACTATC GTTCTACTGG CCAATGACTC CAAAGGCATG
    ATCTTCTACA ATGGTCAGAA GACAGACGGC AAAGGGGATT TCATCTCGCT CTCTCTTAAT
    GACGGCATCC TGGAGTTTAG ATATGACCTG GGCAAAGGGC CAGCTGTCAT TAGGAGTAAA
    GAAAAAATCA AGCTGAACGA GTGGAACACA GTGAATTTGG AACGGGCCAG CAGAAAAGGA
    GAGATTAGCA TTAACGGCAA AGACCCTGTC AGAGGAGAGG CGCCGAAATC ACGCAAGAAT
    CAGCACACGG ATCTCAATCT GAAGGAATCG CTCTTTGTGG GTGGAGCTCC TGATTTCCGC
    AAAGTAGCCA GAGCTGCTGC GATCAAAGAT GGCTTCAAAG GGGCCATTCA GAAGATTACT
    TTGATGGGAA TTCCAATCCT GAAAGCTGAT AATGCCCTTT CGTCCACTGA CGTGTCCATG
    TATCCTAATC ATCCCTGCTC CAAAGACGTT TGTGAGAATG GAGGCCGCTG CAACCCCCAG
    CTGAACAGCT ATGAGTGCGC CTGCCGCCAC GGATTCTCCG GACACCACTG CCAGGACACA
    ATATTTGAGA AGTCCGCTGG GGACACGGAG GCCATTGCGT TTGATGGTCA TACATTCATC
    GAATATCACA ACGGAGTCAC CAAAAGCCCT GAGTCACTGG AGAACCCATC TGACCAGAGC
    GAGAAAGCCC TGCTGGTGAA TAAATTCGAG CTGAGCATCA GGACGGAGGC TACGCATGGT
    CTGATTTTGT GGAGCGGAAA GGGAGTTGAG CGCTCTGACT ACATCGCCCT CGCCATTGTG
    GATGGAAGAG TACAGATGAC CTATGACCTC GGCTCCAAGC CTGTGGTGCT GCGCTCCACT
    GTACGGGTCA ACACCAACCA CTGGATACGA ATTAAGGCCA GCAGAGCGCT CAGAGATGGC
    TCTCTGCAGG TTGGTAATGA GGCAGCTGTC ACTGGGTCTT CACCCTTGGC TGCCACACAG
    CTGGACACAG ACGGGGCTCT CTGGTTGGGT GGACTGGAGG AGCTGTCTGT GGCTCGACGG
    CTGCCCAAAG CTTACAGTAC AGGCTTCATT GGCTGCGTCA AAGACGTGAT AGTGGACGGC
    GTGGAGCTGC ATCTGGTGGA GGACGCCTTA AATACTCCCA AAATATCACA CTGTTCTGCC
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295141.1
    CDS1636..7461
    Translation

    Target ORF information:

    RefSeq Version XM_009296866.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296866.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGACCAGTT GTCAGTACCC CACGCCGCCC CCAGAGCGGG ACCGCATGTA CCAGCACAAA 
    GTGTCGCTGG TCGTGCGATA CTTCATGATT CCTTGCAACA TCTGTCTCAT ACTGCTGGCC
    ACATCCACGC TCGGCTTTGC TGTACTCCTC TTCCTCAATA ACTATAAACC TGTTCACTTC
    ACACCGGCAC CTCCTCCTGA TGGTTGCAGA GGGAAGTTGT GCGGATTTGG AGCGGTGTGT
    GAGAGAGACC AAGCTGACCC ATCTAAAGGA GAGTGTGTGT GTAAGAAGAT TGTGTGTACA
    TCTGTGGTGG CGCCAGTGTG CGGATCTGAC TCCTCCACCT ATTCCAATGA GTGTGAGCTG
    GAGAAGGCTC AGTGCAATAC ACAGAGACGC ATCAAGGTGA TGCGCAAAGG CCCCTGCGCC
    CTCAAGGACC CGTGCAGTGA GGTAACCTGT AGCTTTGGCA GCACCTGCAT CCAGTCATCG
    GATGGTCTGT CCGCCAAGTG TATGTGTCCT CTGAGCTGTG AAAACGTCCC CAAGCATGTG
    GTGTGTGGCA GTGACGGCCT GGACTACCAA AGCGAATGTG AACTCAACAT GAAGGCCTGC
    GCCACTCAGA AGAACATCCG TGTGCATCAC CAGGGCCGCT GCGATCCTTG CAGTGACACT
    CTGAACAGCT TAAGCACAGC CTGTCGAGTC AACCCAAAGA CCTACCAGCA GATTACCTTT
    GCCCCGGCTG AGTCTTGTCC GCCTGACGAA ATGCCCATCT GTGCCAGCGA CGGACACACC
    TACGAGAGCA TGTGTCAGAT GGAGCGCACT GCCCTGAAGA ACAACCTGGA GCTCAAAAAG
    GTCTCTTTAG GCAGCTGCAA GGAAAAAGGG GGCTGCCCGA ATGGTTGTAA GTTCAACGCC
    ATATGTTTGC TGGATAATGG AGAGTTTCGG TGCTCCTGCG ATCCCATACA ATGCGACGGC
    ACGTACAAGC CCCTGTGCGG AAAGGATGGC AAGACATACC CCAACGACTG CGAGAGGAAA
    CTGCAGGAAT GCCGGACCCA AAAAGACATC CCTGTCAAAC AACAAGGGCC CTGTGATCTT
    AACCTGGAGA GCCCCTGCCT GAAGAAGACC TGCGAGTTTG GAGCCGTGTG CGTGGTGAAG
    AACAGCGAAG CAGTGTGTGA ATGCTCAGAC GCCTGTCCGC AGGACCAAGA CCCTGTTTGC
    GGCAGCGATG GGCACACGTA CAGCAGCTCA TGTCAAATGA AGGCCATGGG ATGCGCTCTT
    CAGAAACAGA TACAGATGCA ACACAAAGGA CCATGTGCAG ATGAGTCCTG CACCAACTGC
    TCGTTTGGCG CGATCTGTGA TGCCCAGACT GGCCGGTGCG TGTGCCCTAA AGGCTGTTTG
    GAAACACGGC AGCCCGTATG CGGCAGCGAC GGCACCACGT ATGACAACGA GTGCAAGCTC
    AACGTCCAAG CCTGCACTAA ACAGCTGGAT CTCAAGGTGG TGGCCCATGG AGAATGCAAA
    ACCTGTGGCA GCACTGTGTG TAGCTGGGGG TCGCAGTGCG TGAACAACAA GTGTGAGTGT
    CAGCAGTGCA CTGGTCAGCC GGTGAAACCC GTGTGTGGCA GCGACGGCAA CACATACAAC
    AACGACTGCG AGCTGAGACT GGCCTCCTGT CAGAAACAGA GGAAGATAGA GGTGGCCAAG
    CCTGGCGTCT GCGATGAAGA CTGTGGTTCA GGAGGCTCCG GCTCGGGATC TGAGACTTGT
    GAACAGGACC GATGTCGCCG ATATGGAGGA ACGTGGGACG ATGACATGGA GGACGAGCGA
    TGTGTTTGTG AACTAAACTG TCAGATTGTG CTGCACAGCC CGGTGTGCGG CTCTGATGGA
    AACACGTACT CCAGCGACTG TGAGCTCAAG AAGACCAGGT GCGAAAAGCA AATAGACCTG
    AGTGTCCAAA GCCAAGGGCC CTGTCCAACT ATCGTATCAC CACCTCCAGA GCTTGTTGCC
    TCCCAGCACT GTAGCCAAAC AGTTTATGGA TGTTGTGAAG ATAACAAGAC CGCTGCCCTG
    GGTGTTGGAC TGGCTGGATG TCCTAGTATG TGTCAGTGCA ATCATTACGG CTCATACGGA
    GGCACATGTG ACCCCTCGAC TGGCCAGTGT TCCTGTAAAC CTGGTGTCGG TGGACTGAAG
    TGCGACCGCT GTGAGCCCGG ATTTTGGAAC TTCCGTGGCA TTGTCACAGA AAACATGAGC
    GGCTGCACAC CGTGCAACTG TGATGGAGTG GGTTCGGTTC GTGATGACTG CGAGCAGATG
    TCAGGCCTGT GCTCCTGTAA GCCTGGAGTT AAGGGCATGA AATGCAACGT GTGCCCTGAT
    GGCAGCAAGA TGGGAGTCAA CGGCTGTGAC AAAGGACCTA AGGCTCCCAA GTCCTGTGCT
    GAGCTTGTGT GCCACTTTGG GTCTTCATGC GTGGAGGTGA ATGGACAGGC TCACTGTGAG
    TGTCCTTCAC CAGATTGTGA TGAGAAAAAC AAGACCAAGG TATGTGGTTC TGATGGGGTC
    ACCTATGCAG ACCGGTGCCA GCTGAAGACC ATCGCATGCC GACAAGATAA AGAGATCAAG
    GTGGAGCATT TAGGCCAGTG TAAAGAATCC ATTGAGCTGA CGGCCAAACC CACCCCCTTC
    CCAACCACCC ACCGCACCAC CCCTCCCACC GCTCACGTCA AGGTCACCAA ACAGCGACAT
    GGCCACCGCA CCACCACTCC GGACAGCTAT GTGGTGGAGG CAATGCCTCC ACCGAGGTTG
    GATGGAGCGC GAAAGTTTTC CAGCCCTTTC ACCACCCACC GTCCCACTAC ACCCTACAAA
    CCACTCCGCA CCTCATCCCC ACCTCTGGAC TTTGACGAGT CGGGCAGTGG AGAGCCCAGT
    GGGGATGATG AGATGGTGAG CGGAATGGAG GAGAGCGGAG AAGAGCCACT TGGTACAACA
    GTAGCTACTA CCACTCTTCC CACAGCTGAG GAGCGATCGT CCTGTGACAA CACAGCGTTC
    GGCTGCTGTC CCGATGGGAA GACGGCCGCC GTCAACTCAG AGGGCACAAA CTGCCCATCC
    ACCATGCGTT TCAGCGGCTT CCTGCACCTG GATAAGGTTG AGGGTCAGGA GGTCTTCTAC
    ACGCCTGAGA TGGAGGACCC CAAATCGGAA CTGTTTGGTG AAACGGCCCG AAGCATCGAG
    AGCGCTATGA ATGAGTTGTT CCGCAAGTCT GATGTCCAGA AAGACTTCCA GAGTGTCCGT
    GTGAGGAATC TATCCCCCAG CAACTCCATA ATAGCACATT TCGAGGCTCA CTTCGACCCA
    GACACAAGGT TCAATGTGGG GGATATTGAG GGAGCCCTTC TCAAACAGCT TAAGGCGTCT
    AAAGACACTG GCATCGTTGT GAAGAAACCA GAGGAAGAGA ACATTCGCAT CAACAACTAT
    GGTCTTTCAA CTGTTCCTTT CTTTACCACC ACAACAACCA CTGTGGCATC TGTCACGACT
    CCCACTACCA CCACCACCAC AATGTCTCCC AGTTCTGCTT ATACCACTCT CCGGCCCATC
    ACTACTAGAC GACCCTTCAC AAGAACCACC ACGCCAGCAC CAGCCACCAC CACTGCACCA
    CATGTGCCCA AGACCACAGT TTCGCTTATG AACAGACTAG GGCCCCACGC TCACAGTCCA
    TGTGACTCTC AGCCCTGCCG ACATGGAGGA ACCTGTGAAG AAGACGACAA TGACTTCACC
    TGTATCTGTC CAGCAGGACG AGGAGGAGCC GTGTGTGAGA AAGTTATAAA GTACTTCATA
    CCATCCTTTG GTGGAAAGTC TTACATGGCG TTCCAGACCA TGAAGGCCTA CCATACTGTA
    CGCATTGCCA TGGAGTTCAG GGCTTCGGAA ATGACTGGGA TATTGCTCTA CAATGGGCAA
    AAAAGCAAAA AGGACTTTCT TTCTCTGACT CTAGTTGAAG GATATGTGGA GCTCAGGTTT
    AACACTGGTT CAGGGACTGC TTCAGTTATC AGTAAAGTCC TGGTAAAGCC GGGTCGCTGG
    CATCAGCTTG TTGTAGTACG TAACAGGCGC AATGCCATGC TGAGCGTGGA CAATGAGCCC
    AGCGTTGAAG GACAAAGTCC TCCAGGAACA GATGGACTCA ACCTGGACAC AGACCTTTTC
    ATCGGCGGAG TGCTTGATGA CATGAGTGCA GACGTAAAGG AGAGGACATC AGTTTCAGCA
    GGTCTGGTGG GCTGCATCCG CATGCTGGAC GTGAACAACC TTGTCTATAA TCTGCAAGAG
    AATGGCGGCA GCGTTCTGTA CGGTTCGGCC ATTGGCGAGT GTGGCAACAA CCCATGCCTG
    CCAAACCCCT GCAAGAACGG AGCGTCCTGT CAGGTCAAAG AAGCCGAGAT TTTCCACTGC
    AAATGTGTCA ACGGTTTCTC TGGCCCGACC TGCGCTGATG CCCACAATCC CTGCGACCCC
    AATAAATGCC ACCCGCCATC ACGGTGCCAG GTCCTTCCAG AGGGAGGTTA CAAATGCGAA
    TGTCCGATGG GACGTGAAGG CAGGCACTGT GAAAAAGTGT CAGACAAGGG AGGCGCTTTC
    ATTCCTTACT TCACTGGAGA TTCTTTTCTG GAGCTCAAAG GCCTCCATAC GTATAACCAG
    GATCTCAGGC AAAAATTCAG CATGACTATC GTTCTACTGG CCAATGACTC CAAAGGCATG
    ATCTTCTACA ATGGTCAGAA GACAGACGGC AAAGGGGATT TCATCTCGCT CTCTCTTAAT
    GACGGCATCC TGGAGTTTAG ATATGACCTG GGCAAAGGGC CAGCTGTCAT TAGGAGTAAA
    GAAAAAATCA AGCTGAACGA GTGGAACACA GTGAATTTGG AACGGGCCAG CAGAAAAGGA
    GAGATTAGCA TTAACGGCAA AGACCCTGTC AGAGGAGAGG CGCCGAAATC ACGCAAGAAT
    CAGCACACGG ATCTCAATCT GAAGGAATCG CTCTTTGTGG GTGGAGCTCC TGATTTCCGC
    AAAGTAGCCA GAGCTGCTGC GATCAAAGAT GGCTTCAAAG GGGCCATTCA GAAGATTACT
    TTGATGGGAA TTCCAATCCT GAAAGCTGAT AATGCCCTTT CGTCCACTGA CGTGTCCATG
    TATCCTAATC ATCCCTGCTC CAAAGACGTT TGTGAGAATG GAGGCCGCTG CAACCCCCAG
    CTGAACAGCT ATGAGTGCGC CTGCCGCCAC GGATTCTCCG GACACCACTG CCAGGACACA
    ATATTTGAGA AGTCCGCTGG GGACACGGAG GCCATTGCGT TTGATGGTCA TACATTCATC
    GAATATCACA ACGGAGTCAC CAAAAGCCCT GAGTCACTGG AGAACCCATC TGACCAGAGC
    GAGAAAGCCC TGCTGGTGAA TAAATTCGAG CTGAGCATCA GGACGGAGGC TACGCATGGT
    CTGATTTTGT GGAGCGGAAA GGGAGTTGAG CGCTCTGACT ACATCGCCCT CGCCATTGTG
    GATGGAAGAG TACAGATGAC CTATGACCTC GGCTCCAAGC CTGTGGTGCT GCGCTCCACT
    GTACGGGTCA ACACCAACCA CTGGATACGA ATTAAGGCCA GCAGAGCGCT CAGAGATGGC
    TCTCTGCAGG TTGGTAATGA GGCAGCTGTC ACTGGGTCTT CACCCTTGGC TGCCACACAG
    CTGGACACAG ACGGGGCTCT CTGGTTGGGT GGACTGGAGG AGCTGTCTGT GGCTCGACGG
    CTGCCCAAAG CTTACAGTAC AGGCTTCATT GGCTGCGTCA AAGACGTGAT AGTGGACGGC
    GTGGAGCTGC ATCTGGTGGA GGACGCCTTA AATACTCCCA AAATATCACA CTGTTCTGCC
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44581
    Clone ID Related Accession (Same CDS sequence) XM_009296867.2 , XM_009296867.3
    Accession Version XM_009296867.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5781bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product agrin isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296867.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGACCAGTT GTCAGTACCC CACGCCGCCC CCAGAGCGGG ACCGCATGTA CCAGCACAAA 
    GTGTCGCTGG TCGTGCGATA CTTCATGATT CCTTGCAACA TCTGTCTCAT ACTGCTGGCC
    ACATCCACGC TCGGCTTTGC TGTACTCCTC TTCCTCAATA ACTATAAACC TGTTCACTTC
    ACACCGGCAC CTCCTCCTGA TGGTTGCAGA GGGAAGTTGT GCGGATTTGG AGCGGTGTGT
    GAGAGAGACC AAGCTGACCC ATCTAAAGGA GAGTGTGTGT GTAAGAAGAT TGTGTGTACA
    TCTGTGGTGG CGCCAGTGTG CGGATCTGAC TCCTCCACCT ATTCCAATGA GTGTGAGCTG
    GAGAAGGCTC AGTGCAATAC ACAGAGACGC ATCAAGGTGA TGCGCAAAGG CCCCTGCGCC
    CTCAAGGACC CGTGCAGTGA GGTAACCTGT AGCTTTGGCA GCACCTGCAT CCAGTCATCG
    GATGGTCTGT CCGCCAAGTG TATGTGTCCT CTGAGCTGTG AAAACGTCCC CAAGCATGTG
    GTGTGTGGCA GTGACGGCCT GGACTACCAA AGCGAATGTG AACTCAACAT GAAGGCCTGC
    GCCACTCAGA AGAACATCCG TGTGCATCAC CAGGGCCGCT GCGATCCTTG CAGTGACACT
    CTGAACAGCT TAAGCACAGC CTGTCGAGTC AACCCAAAGA CCTACCAGCA GATTACCTTT
    GCCCCGGCTG AGTCTTGTCC GCCTGACGAA ATGCCCATCT GTGCCAGCGA CGGACACACC
    TACGAGAGCA TGTGTCAGAT GGAGCGCACT GCCCTGAAGA ACAACCTGGA GCTCAAAAAG
    GTCTCTTTAG GCAGCTGCAA GGAAAAAGGG GGCTGCCCGA ATGGTTGTAA GTTCAACGCC
    ATATGTTTGC TGGATAATGG AGAGTTTCGG TGCTCCTGCG ATCCCATACA ATGCGACGGC
    ACGTACAAGC CCCTGTGCGG AAAGGATGGC AAGACATACC CCAACGACTG CGAGAGGAAA
    CTGCAGGAAT GCCGGACCCA AAAAGACATC CCTGTCAAAC AACAAGGGCC CTGTGATCTT
    AACCTGGAGA GCCCCTGCCT GAAGAAGACC TGCGAGTTTG GAGCCGTGTG CGTGGTGAAG
    AACAGCGAAG CAGTGTGTGA ATGCTCAGAC GCCTGTCCGC AGGACCAAGA CCCTGTTTGC
    GGCAGCGATG GGCACACGTA CAGCAGCTCA TGTCAAATGA AGGCCATGGG ATGCGCTCTT
    CAGAAACAGA TACAGATGCA ACACAAAGGA CCATGTGCAG ATGAGTCCTG CACCAACTGC
    TCGTTTGGCG CGATCTGTGA TGCCCAGACT GGCCGGTGCG TGTGCCCTAA AGGCTGTTTG
    GAAACACGGC AGCCCGTATG CGGCAGCGAC GGCACCACGT ATGACAACGA GTGCAAGCTC
    AACGTCCAAG CCTGCACTAA ACAGCTGGAT CTCAAGGTGG TGGCCCATGG AGAATGCAAA
    ACCTGTGGCA GCACTGTGTG TAGCTGGGGG TCGCAGTGCG TGAACAACAA GTGTGAGTGT
    CAGCAGTGCA CTGGTCAGCC GGTGAAACCC GTGTGTGGCA GCGACGGCAA CACATACAAC
    AACGACTGCG AGCTGAGACT GGCCTCCTGT CAGAAACAGA GGAAGATAGA GGTGGCCAAG
    CCTGGCGTCT GCGATGAAGA CTGTGGTTCA GGAGGCTCCG GCTCGGGATC TGAGACTTGT
    GAACAGGACC GATGTCGCCG ATATGGAGGA ACGTGGGACG ATGACATGGA GGACGAGCGA
    TGTGTTTGTG AACTAAACTG TCAGATTGTG CTGCACAGCC CGGTGTGCGG CTCTGATGGA
    AACACGTACT CCAGCGACTG TGAGCTCAAG AAGACCAGGT GCGAAAAGCA AATAGACCTG
    AGTGTCCAAA GCCAAGGGCC CTGTCCAACT ATCGTATCAC CACCTCCAGA GCTTGTTGCC
    TCCCAGCACT GTAGCCAAAC AGTTTATGGA TGTTGTGAAG ATAACAAGAC CGCTGCCCTG
    GGTGTTGGAC TGGCTGGATG TCCTAGTATG TGTCAGTGCA ATCATTACGG CTCATACGGA
    GGCACATGTG ACCCCTCGAC TGGCCAGTGT TCCTGTAAAC CTGGTGTCGG TGGACTGAAG
    TGCGACCGCT GTGAGCCCGG ATTTTGGAAC TTCCGTGGCA TTGTCACAGA AAACATGAGC
    GGCTGCACAC CGTGCAACTG TGATGGAGTG GGTTCGGTTC GTGATGACTG CGAGCAGATG
    TCAGGCCTGT GCTCCTGTAA GCCTGGAGTT AAGGGCATGA AATGCAACGT GTGCCCTGAT
    GGCAGCAAGA TGGGAGTCAA CGGCTGTGAC AAAGGACCTA AGGCTCCCAA GTCCTGTGCT
    GAGCTTGTGT GCCACTTTGG GTCTTCATGC GTGGAGGTGA ATGGACAGGC TCACTGTGAG
    TGTCCTTCAC CAGATTGTGA TGAGAAAAAC AAGACCAAGG TATGTGGTTC TGATGGGGTC
    ACCTATGCAG ACCGGTGCCA GCTGAAGACC ATCGCATGCC GACAAGATAA AGAGATCAAG
    GTGGAGCATT TAGGCCAGTG TAAAGAATCC ATTGAGCTGA CGGCCAAACC CACCCCCTTC
    CCAACCACCC ACCGCACCAC CCCTCCCACC GCTCACGTCA AGGTCACCAA ACAGCGACAT
    GGCCACCGCA CCACCACTCC GGACAGCTAT GTGGTGGAGG CAATGCCTCC ACCGAGGTTG
    GATGGAGCGC GAAAGTTTTC CAGCCCTTTC ACCACCCACC GTCCCACTAC ACCCTACAAA
    CCACTCCGCA CCTCATCCCC ACCTCTGGAC TTTGACGAGT CGGGCAGTGG AGAGCCCAGT
    GGGGATGATG AGATGGTGAG CGGAATGGAG GAGAGCGGAG AAGAGCCACT TGGTACAACA
    GTAGCTACTA CCACTCTTCC CACAGCTGAG GAGCGATCGT CCTGTGACAA CACAGCGTTC
    GGCTGCTGTC CCGATGGGAA GACGGCCGCC GTCAACTCAG AGGGCACAAA CTGCCCATCC
    ACCATGCGTT TCAGCGGCTT CCTGCACCTG GATAAGGTTG AGGGTCAGGA GGTCTTCTAC
    ACGCCTGAGA TGGAGGACCC CAAATCGGAA CTGTTTGGTG AAACGGCCCG AAGCATCGAG
    AGCGCTATGA ATGAGTTGTT CCGCAAGTCT GATGTCCAGA AAGACTTCCA GAGTGTCCGT
    GTGAGGAATC TATCCCCCAG CAACTCCATA ATAGCACATT TCGAGGCTCA CTTCGACCCA
    GACACAAGGT TCAATGTGGG GGATATTGAG GGAGCCCTTC TCAAACAGCT TAAGGCGTCT
    AAAGACACTG GCATCGTTGT GAAGAAACCA GAGGAAGAGA ACATTCGCAT CAACAACTAT
    GGTCTTTCAA CTGTTCCTTT CTTTACCACC ACAACAACCA CTGTGGCATC TGTCACGACT
    CCCACTACCA CCACCACCAC AATGTCTCCC AGTTCTGCTT ATACCACTCT CCGGCCCATC
    ACTACTAGAC GACCCTTCAC AAGAACCACC ACGCCAGCAC CAGCCACCAC CACTGCACCA
    CATGTGCCCA AGACCACAGT TTCGCTTATG AACAGACTAG GGCCCCACGC TCACAGTCCA
    TGTGACTCTC AGCCCTGCCG ACATGGAGGA ACCTGTGAAG AAGACGACAA TGACTTCACC
    TGTATCTGTC CAGCAGGACG AGGAGGAGCC GTGTGTGAGA AAGTTATAAA GTACTTCATA
    CCATCCTTTG GTGGAAAGTC TTACATGGCG TTCCAGACCA TGAAGGCCTA CCATACTGTA
    CGCATTGCCA TGGAGTTCAG GGCTTCGGAA ATGACTGGGA TATTGCTCTA CAATGGGCAA
    AAAAGCAAAA AGGACTTTCT TTCTCTGACT CTAGTTGAAG GATATGTGGA GCTCAGGTTT
    AACACTGGTT CAGGGACTGC TTCAGTTATC AGTAAAGTCC TGGTAAAGCC GGGTCGCTGG
    CATCAGCTTG TTGTAGTACG TAACAGGCGC AATGCCATGC TGAGCGTGGA CAATGAGCCC
    AGCGTTGAAG GACAAAGTCC TCCAGGAACA GATGGACTCA ACCTGGACAC AGACCTTTTC
    ATCGGCGGAG TGCTTGATGA CATGAGTGCA GACGTAAAGG AGAGGACATC AGTTTCAGCA
    GGTCTGGTGG GCTGCATCCG CATGCTGGAC GTGAACAACC TTGTCTATAA TCTGCAAGAG
    AATGGCGGCA GCGTTCTGTA CGGTTCGGCC ATTGGCGAGT GTGGCAACAA CCCATGCCTG
    CCAAACCCCT GCAAGAACGG AGCGTCCTGT CAGGTCAAAG AAGCCGAGAT TTTCCACTGC
    AAATGTGTCA ACGGTTTCTC TGGCCCGACC TGCGCTGATG CCCACAATCC CTGCGACCCC
    AATAAATGCC ACCCGCCATC ACGGTGCCAG GTCCTTCCAG AGGGAGGTTA CAAATGCGAA
    TGTCCGATGG GACGTGAAGG CAGGCACTGT GAAAAAGTGT CAGACAAGGG AGGCGCTTTC
    ATTCCTTACT TCACTGGAGA TTCTTTTCTG GAGCTCAAAG GCCTCCATAC GTATAACCAG
    GATCTCAGGC AAAAATTCAG CATGACTATC GTTCTACTGG CCAATGACTC CAAAGGCATG
    ATCTTCTACA ATGGTCAGAA GACAGACGGC AAAGGGGATT TCATCTCGCT CTCTCTTAAT
    GACGGCATCC TGGAGTTTAG ATATGACCTG GGCAAAGGGC CAGCTGTCAT TAGGAGTAAA
    GAAAAAATCA AGCTGAACGA GTGGAACACA GTGAATTTGG AACGGGCCAG CAGAAAAGGA
    GAGATTAGCA TTAACGGCAA AGACCCTGTC AGAGGAGAGG CGCCGAATCA GCACACGGAT
    CTCAATCTGA AGGAATCGCT CTTTGTGGGT GGAGCTCCTG ATTTCCGCAA AGTAGCCAGA
    GCTGCTGCGA TCAAAGATGG CTTCAAAGGG GCCATTCAGA AGATTACTTT GATGGGAATT
    CCAATCCTGA AAGCTGATAA TGCCCTTTCG TCCACTGACG TGTCCATGTA TCCTAATCAT
    CCCTGCTCCA AAGACGTTTG TGAGAATGGA GGCCGCTGCA ACCCCCAGCT GAACAGCTAT
    GAGTGCGCCT GCCGCCACGG ATTCTCCGGA CACCACTGCC AGGACACAAT ATTTGAGAAG
    TCCGCTGGGG ACACGGAGGC CATTGCGTTT GATGGTCATA CATTCATCGA ATATCACAAC
    GGAGTCACCA AAAGCGAGAA AGCCCTGCTG GTGAATAAAT TCGAGCTGAG CATCAGGACG
    GAGGCTACGC ATGGTCTGAT TTTGTGGAGC GGAAAGGGAG TTGAGCGCTC TGACTACATC
    GCCCTCGCCA TTGTGGATGG AAGAGTACAG ATGACCTATG ACCTCGGCTC CAAGCCTGTG
    GTGCTGCGCT CCACTGTACG GGTCAACACC AACCACTGGA TACGAATTAA GGCCAGCAGA
    GCGCTCAGAG ATGGCTCTCT GCAGGTTGGT AATGAGGCAG CTGTCACTGG GTCTTCACCC
    TTGGCTGCCA CACAGCTGGA CACAGACGGG GCTCTCTGGT TGGGTGGACT GGAGGAGCTG
    TCTGTGGCTC GACGGCTGCC CAAAGCTTAC AGTACAGGCT TCATTGGCTG CGTCAAAGAC
    GTGATAGTGG ACGGCGTGGA GCTGCATCTG GTGGAGGACG CCTTAAATAC TCCCAAAATA
    TCACACTGTT CTGCCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295142.1
    CDS1636..7416
    Translation

    Target ORF information:

    RefSeq Version XM_009296867.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296867.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGACCAGTT GTCAGTACCC CACGCCGCCC CCAGAGCGGG ACCGCATGTA CCAGCACAAA 
    GTGTCGCTGG TCGTGCGATA CTTCATGATT CCTTGCAACA TCTGTCTCAT ACTGCTGGCC
    ACATCCACGC TCGGCTTTGC TGTACTCCTC TTCCTCAATA ACTATAAACC TGTTCACTTC
    ACACCGGCAC CTCCTCCTGA TGGTTGCAGA GGGAAGTTGT GCGGATTTGG AGCGGTGTGT
    GAGAGAGACC AAGCTGACCC ATCTAAAGGA GAGTGTGTGT GTAAGAAGAT TGTGTGTACA
    TCTGTGGTGG CGCCAGTGTG CGGATCTGAC TCCTCCACCT ATTCCAATGA GTGTGAGCTG
    GAGAAGGCTC AGTGCAATAC ACAGAGACGC ATCAAGGTGA TGCGCAAAGG CCCCTGCGCC
    CTCAAGGACC CGTGCAGTGA GGTAACCTGT AGCTTTGGCA GCACCTGCAT CCAGTCATCG
    GATGGTCTGT CCGCCAAGTG TATGTGTCCT CTGAGCTGTG AAAACGTCCC CAAGCATGTG
    GTGTGTGGCA GTGACGGCCT GGACTACCAA AGCGAATGTG AACTCAACAT GAAGGCCTGC
    GCCACTCAGA AGAACATCCG TGTGCATCAC CAGGGCCGCT GCGATCCTTG CAGTGACACT
    CTGAACAGCT TAAGCACAGC CTGTCGAGTC AACCCAAAGA CCTACCAGCA GATTACCTTT
    GCCCCGGCTG AGTCTTGTCC GCCTGACGAA ATGCCCATCT GTGCCAGCGA CGGACACACC
    TACGAGAGCA TGTGTCAGAT GGAGCGCACT GCCCTGAAGA ACAACCTGGA GCTCAAAAAG
    GTCTCTTTAG GCAGCTGCAA GGAAAAAGGG GGCTGCCCGA ATGGTTGTAA GTTCAACGCC
    ATATGTTTGC TGGATAATGG AGAGTTTCGG TGCTCCTGCG ATCCCATACA ATGCGACGGC
    ACGTACAAGC CCCTGTGCGG AAAGGATGGC AAGACATACC CCAACGACTG CGAGAGGAAA
    CTGCAGGAAT GCCGGACCCA AAAAGACATC CCTGTCAAAC AACAAGGGCC CTGTGATCTT
    AACCTGGAGA GCCCCTGCCT GAAGAAGACC TGCGAGTTTG GAGCCGTGTG CGTGGTGAAG
    AACAGCGAAG CAGTGTGTGA ATGCTCAGAC GCCTGTCCGC AGGACCAAGA CCCTGTTTGC
    GGCAGCGATG GGCACACGTA CAGCAGCTCA TGTCAAATGA AGGCCATGGG ATGCGCTCTT
    CAGAAACAGA TACAGATGCA ACACAAAGGA CCATGTGCAG ATGAGTCCTG CACCAACTGC
    TCGTTTGGCG CGATCTGTGA TGCCCAGACT GGCCGGTGCG TGTGCCCTAA AGGCTGTTTG
    GAAACACGGC AGCCCGTATG CGGCAGCGAC GGCACCACGT ATGACAACGA GTGCAAGCTC
    AACGTCCAAG CCTGCACTAA ACAGCTGGAT CTCAAGGTGG TGGCCCATGG AGAATGCAAA
    ACCTGTGGCA GCACTGTGTG TAGCTGGGGG TCGCAGTGCG TGAACAACAA GTGTGAGTGT
    CAGCAGTGCA CTGGTCAGCC GGTGAAACCC GTGTGTGGCA GCGACGGCAA CACATACAAC
    AACGACTGCG AGCTGAGACT GGCCTCCTGT CAGAAACAGA GGAAGATAGA GGTGGCCAAG
    CCTGGCGTCT GCGATGAAGA CTGTGGTTCA GGAGGCTCCG GCTCGGGATC TGAGACTTGT
    GAACAGGACC GATGTCGCCG ATATGGAGGA ACGTGGGACG ATGACATGGA GGACGAGCGA
    TGTGTTTGTG AACTAAACTG TCAGATTGTG CTGCACAGCC CGGTGTGCGG CTCTGATGGA
    AACACGTACT CCAGCGACTG TGAGCTCAAG AAGACCAGGT GCGAAAAGCA AATAGACCTG
    AGTGTCCAAA GCCAAGGGCC CTGTCCAACT ATCGTATCAC CACCTCCAGA GCTTGTTGCC
    TCCCAGCACT GTAGCCAAAC AGTTTATGGA TGTTGTGAAG ATAACAAGAC CGCTGCCCTG
    GGTGTTGGAC TGGCTGGATG TCCTAGTATG TGTCAGTGCA ATCATTACGG CTCATACGGA
    GGCACATGTG ACCCCTCGAC TGGCCAGTGT TCCTGTAAAC CTGGTGTCGG TGGACTGAAG
    TGCGACCGCT GTGAGCCCGG ATTTTGGAAC TTCCGTGGCA TTGTCACAGA AAACATGAGC
    GGCTGCACAC CGTGCAACTG TGATGGAGTG GGTTCGGTTC GTGATGACTG CGAGCAGATG
    TCAGGCCTGT GCTCCTGTAA GCCTGGAGTT AAGGGCATGA AATGCAACGT GTGCCCTGAT
    GGCAGCAAGA TGGGAGTCAA CGGCTGTGAC AAAGGACCTA AGGCTCCCAA GTCCTGTGCT
    GAGCTTGTGT GCCACTTTGG GTCTTCATGC GTGGAGGTGA ATGGACAGGC TCACTGTGAG
    TGTCCTTCAC CAGATTGTGA TGAGAAAAAC AAGACCAAGG TATGTGGTTC TGATGGGGTC
    ACCTATGCAG ACCGGTGCCA GCTGAAGACC ATCGCATGCC GACAAGATAA AGAGATCAAG
    GTGGAGCATT TAGGCCAGTG TAAAGAATCC ATTGAGCTGA CGGCCAAACC CACCCCCTTC
    CCAACCACCC ACCGCACCAC CCCTCCCACC GCTCACGTCA AGGTCACCAA ACAGCGACAT
    GGCCACCGCA CCACCACTCC GGACAGCTAT GTGGTGGAGG CAATGCCTCC ACCGAGGTTG
    GATGGAGCGC GAAAGTTTTC CAGCCCTTTC ACCACCCACC GTCCCACTAC ACCCTACAAA
    CCACTCCGCA CCTCATCCCC ACCTCTGGAC TTTGACGAGT CGGGCAGTGG AGAGCCCAGT
    GGGGATGATG AGATGGTGAG CGGAATGGAG GAGAGCGGAG AAGAGCCACT TGGTACAACA
    GTAGCTACTA CCACTCTTCC CACAGCTGAG GAGCGATCGT CCTGTGACAA CACAGCGTTC
    GGCTGCTGTC CCGATGGGAA GACGGCCGCC GTCAACTCAG AGGGCACAAA CTGCCCATCC
    ACCATGCGTT TCAGCGGCTT CCTGCACCTG GATAAGGTTG AGGGTCAGGA GGTCTTCTAC
    ACGCCTGAGA TGGAGGACCC CAAATCGGAA CTGTTTGGTG AAACGGCCCG AAGCATCGAG
    AGCGCTATGA ATGAGTTGTT CCGCAAGTCT GATGTCCAGA AAGACTTCCA GAGTGTCCGT
    GTGAGGAATC TATCCCCCAG CAACTCCATA ATAGCACATT TCGAGGCTCA CTTCGACCCA
    GACACAAGGT TCAATGTGGG GGATATTGAG GGAGCCCTTC TCAAACAGCT TAAGGCGTCT
    AAAGACACTG GCATCGTTGT GAAGAAACCA GAGGAAGAGA ACATTCGCAT CAACAACTAT
    GGTCTTTCAA CTGTTCCTTT CTTTACCACC ACAACAACCA CTGTGGCATC TGTCACGACT
    CCCACTACCA CCACCACCAC AATGTCTCCC AGTTCTGCTT ATACCACTCT CCGGCCCATC
    ACTACTAGAC GACCCTTCAC AAGAACCACC ACGCCAGCAC CAGCCACCAC CACTGCACCA
    CATGTGCCCA AGACCACAGT TTCGCTTATG AACAGACTAG GGCCCCACGC TCACAGTCCA
    TGTGACTCTC AGCCCTGCCG ACATGGAGGA ACCTGTGAAG AAGACGACAA TGACTTCACC
    TGTATCTGTC CAGCAGGACG AGGAGGAGCC GTGTGTGAGA AAGTTATAAA GTACTTCATA
    CCATCCTTTG GTGGAAAGTC TTACATGGCG TTCCAGACCA TGAAGGCCTA CCATACTGTA
    CGCATTGCCA TGGAGTTCAG GGCTTCGGAA ATGACTGGGA TATTGCTCTA CAATGGGCAA
    AAAAGCAAAA AGGACTTTCT TTCTCTGACT CTAGTTGAAG GATATGTGGA GCTCAGGTTT
    AACACTGGTT CAGGGACTGC TTCAGTTATC AGTAAAGTCC TGGTAAAGCC GGGTCGCTGG
    CATCAGCTTG TTGTAGTACG TAACAGGCGC AATGCCATGC TGAGCGTGGA CAATGAGCCC
    AGCGTTGAAG GACAAAGTCC TCCAGGAACA GATGGACTCA ACCTGGACAC AGACCTTTTC
    ATCGGCGGAG TGCTTGATGA CATGAGTGCA GACGTAAAGG AGAGGACATC AGTTTCAGCA
    GGTCTGGTGG GCTGCATCCG CATGCTGGAC GTGAACAACC TTGTCTATAA TCTGCAAGAG
    AATGGCGGCA GCGTTCTGTA CGGTTCGGCC ATTGGCGAGT GTGGCAACAA CCCATGCCTG
    CCAAACCCCT GCAAGAACGG AGCGTCCTGT CAGGTCAAAG AAGCCGAGAT TTTCCACTGC
    AAATGTGTCA ACGGTTTCTC TGGCCCGACC TGCGCTGATG CCCACAATCC CTGCGACCCC
    AATAAATGCC ACCCGCCATC ACGGTGCCAG GTCCTTCCAG AGGGAGGTTA CAAATGCGAA
    TGTCCGATGG GACGTGAAGG CAGGCACTGT GAAAAAGTGT CAGACAAGGG AGGCGCTTTC
    ATTCCTTACT TCACTGGAGA TTCTTTTCTG GAGCTCAAAG GCCTCCATAC GTATAACCAG
    GATCTCAGGC AAAAATTCAG CATGACTATC GTTCTACTGG CCAATGACTC CAAAGGCATG
    ATCTTCTACA ATGGTCAGAA GACAGACGGC AAAGGGGATT TCATCTCGCT CTCTCTTAAT
    GACGGCATCC TGGAGTTTAG ATATGACCTG GGCAAAGGGC CAGCTGTCAT TAGGAGTAAA
    GAAAAAATCA AGCTGAACGA GTGGAACACA GTGAATTTGG AACGGGCCAG CAGAAAAGGA
    GAGATTAGCA TTAACGGCAA AGACCCTGTC AGAGGAGAGG CGCCGAATCA GCACACGGAT
    CTCAATCTGA AGGAATCGCT CTTTGTGGGT GGAGCTCCTG ATTTCCGCAA AGTAGCCAGA
    GCTGCTGCGA TCAAAGATGG CTTCAAAGGG GCCATTCAGA AGATTACTTT GATGGGAATT
    CCAATCCTGA AAGCTGATAA TGCCCTTTCG TCCACTGACG TGTCCATGTA TCCTAATCAT
    CCCTGCTCCA AAGACGTTTG TGAGAATGGA GGCCGCTGCA ACCCCCAGCT GAACAGCTAT
    GAGTGCGCCT GCCGCCACGG ATTCTCCGGA CACCACTGCC AGGACACAAT ATTTGAGAAG
    TCCGCTGGGG ACACGGAGGC CATTGCGTTT GATGGTCATA CATTCATCGA ATATCACAAC
    GGAGTCACCA AAAGCGAGAA AGCCCTGCTG GTGAATAAAT TCGAGCTGAG CATCAGGACG
    GAGGCTACGC ATGGTCTGAT TTTGTGGAGC GGAAAGGGAG TTGAGCGCTC TGACTACATC
    GCCCTCGCCA TTGTGGATGG AAGAGTACAG ATGACCTATG ACCTCGGCTC CAAGCCTGTG
    GTGCTGCGCT CCACTGTACG GGTCAACACC AACCACTGGA TACGAATTAA GGCCAGCAGA
    GCGCTCAGAG ATGGCTCTCT GCAGGTTGGT AATGAGGCAG CTGTCACTGG GTCTTCACCC
    TTGGCTGCCA CACAGCTGGA CACAGACGGG GCTCTCTGGT TGGGTGGACT GGAGGAGCTG
    TCTGTGGCTC GACGGCTGCC CAAAGCTTAC AGTACAGGCT TCATTGGCTG CGTCAAAGAC
    GTGATAGTGG ACGGCGTGGA GCTGCATCTG GTGGAGGACG CCTTAAATAC TCCCAAAATA
    TCACACTGTT CTGCCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44581
    Clone ID Related Accession (Same CDS sequence) XM_009296867.2 , XM_009296867.3
    Accession Version XM_009296867.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5781bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product agrin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296867.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGACCAGTT GTCAGTACCC CACGCCGCCC CCAGAGCGGG ACCGCATGTA CCAGCACAAA 
    GTGTCGCTGG TCGTGCGATA CTTCATGATT CCTTGCAACA TCTGTCTCAT ACTGCTGGCC
    ACATCCACGC TCGGCTTTGC TGTACTCCTC TTCCTCAATA ACTATAAACC TGTTCACTTC
    ACACCGGCAC CTCCTCCTGA TGGTTGCAGA GGGAAGTTGT GCGGATTTGG AGCGGTGTGT
    GAGAGAGACC AAGCTGACCC ATCTAAAGGA GAGTGTGTGT GTAAGAAGAT TGTGTGTACA
    TCTGTGGTGG CGCCAGTGTG CGGATCTGAC TCCTCCACCT ATTCCAATGA GTGTGAGCTG
    GAGAAGGCTC AGTGCAATAC ACAGAGACGC ATCAAGGTGA TGCGCAAAGG CCCCTGCGCC
    CTCAAGGACC CGTGCAGTGA GGTAACCTGT AGCTTTGGCA GCACCTGCAT CCAGTCATCG
    GATGGTCTGT CCGCCAAGTG TATGTGTCCT CTGAGCTGTG AAAACGTCCC CAAGCATGTG
    GTGTGTGGCA GTGACGGCCT GGACTACCAA AGCGAATGTG AACTCAACAT GAAGGCCTGC
    GCCACTCAGA AGAACATCCG TGTGCATCAC CAGGGCCGCT GCGATCCTTG CAGTGACACT
    CTGAACAGCT TAAGCACAGC CTGTCGAGTC AACCCAAAGA CCTACCAGCA GATTACCTTT
    GCCCCGGCTG AGTCTTGTCC GCCTGACGAA ATGCCCATCT GTGCCAGCGA CGGACACACC
    TACGAGAGCA TGTGTCAGAT GGAGCGCACT GCCCTGAAGA ACAACCTGGA GCTCAAAAAG
    GTCTCTTTAG GCAGCTGCAA GGAAAAAGGG GGCTGCCCGA ATGGTTGTAA GTTCAACGCC
    ATATGTTTGC TGGATAATGG AGAGTTTCGG TGCTCCTGCG ATCCCATACA ATGCGACGGC
    ACGTACAAGC CCCTGTGCGG AAAGGATGGC AAGACATACC CCAACGACTG CGAGAGGAAA
    CTGCAGGAAT GCCGGACCCA AAAAGACATC CCTGTCAAAC AACAAGGGCC CTGTGATCTT
    AACCTGGAGA GCCCCTGCCT GAAGAAGACC TGCGAGTTTG GAGCCGTGTG CGTGGTGAAG
    AACAGCGAAG CAGTGTGTGA ATGCTCAGAC GCCTGTCCGC AGGACCAAGA CCCTGTTTGC
    GGCAGCGATG GGCACACGTA CAGCAGCTCA TGTCAAATGA AGGCCATGGG ATGCGCTCTT
    CAGAAACAGA TACAGATGCA ACACAAAGGA CCATGTGCAG ATGAGTCCTG CACCAACTGC
    TCGTTTGGCG CGATCTGTGA TGCCCAGACT GGCCGGTGCG TGTGCCCTAA AGGCTGTTTG
    GAAACACGGC AGCCCGTATG CGGCAGCGAC GGCACCACGT ATGACAACGA GTGCAAGCTC
    AACGTCCAAG CCTGCACTAA ACAGCTGGAT CTCAAGGTGG TGGCCCATGG AGAATGCAAA
    ACCTGTGGCA GCACTGTGTG TAGCTGGGGG TCGCAGTGCG TGAACAACAA GTGTGAGTGT
    CAGCAGTGCA CTGGTCAGCC GGTGAAACCC GTGTGTGGCA GCGACGGCAA CACATACAAC
    AACGACTGCG AGCTGAGACT GGCCTCCTGT CAGAAACAGA GGAAGATAGA GGTGGCCAAG
    CCTGGCGTCT GCGATGAAGA CTGTGGTTCA GGAGGCTCCG GCTCGGGATC TGAGACTTGT
    GAACAGGACC GATGTCGCCG ATATGGAGGA ACGTGGGACG ATGACATGGA GGACGAGCGA
    TGTGTTTGTG AACTAAACTG TCAGATTGTG CTGCACAGCC CGGTGTGCGG CTCTGATGGA
    AACACGTACT CCAGCGACTG TGAGCTCAAG AAGACCAGGT GCGAAAAGCA AATAGACCTG
    AGTGTCCAAA GCCAAGGGCC CTGTCCAACT ATCGTATCAC CACCTCCAGA GCTTGTTGCC
    TCCCAGCACT GTAGCCAAAC AGTTTATGGA TGTTGTGAAG ATAACAAGAC CGCTGCCCTG
    GGTGTTGGAC TGGCTGGATG TCCTAGTATG TGTCAGTGCA ATCATTACGG CTCATACGGA
    GGCACATGTG ACCCCTCGAC TGGCCAGTGT TCCTGTAAAC CTGGTGTCGG TGGACTGAAG
    TGCGACCGCT GTGAGCCCGG ATTTTGGAAC TTCCGTGGCA TTGTCACAGA AAACATGAGC
    GGCTGCACAC CGTGCAACTG TGATGGAGTG GGTTCGGTTC GTGATGACTG CGAGCAGATG
    TCAGGCCTGT GCTCCTGTAA GCCTGGAGTT AAGGGCATGA AATGCAACGT GTGCCCTGAT
    GGCAGCAAGA TGGGAGTCAA CGGCTGTGAC AAAGGACCTA AGGCTCCCAA GTCCTGTGCT
    GAGCTTGTGT GCCACTTTGG GTCTTCATGC GTGGAGGTGA ATGGACAGGC TCACTGTGAG
    TGTCCTTCAC CAGATTGTGA TGAGAAAAAC AAGACCAAGG TATGTGGTTC TGATGGGGTC
    ACCTATGCAG ACCGGTGCCA GCTGAAGACC ATCGCATGCC GACAAGATAA AGAGATCAAG
    GTGGAGCATT TAGGCCAGTG TAAAGAATCC ATTGAGCTGA CGGCCAAACC CACCCCCTTC
    CCAACCACCC ACCGCACCAC CCCTCCCACC GCTCACGTCA AGGTCACCAA ACAGCGACAT
    GGCCACCGCA CCACCACTCC GGACAGCTAT GTGGTGGAGG CAATGCCTCC ACCGAGGTTG
    GATGGAGCGC GAAAGTTTTC CAGCCCTTTC ACCACCCACC GTCCCACTAC ACCCTACAAA
    CCACTCCGCA CCTCATCCCC ACCTCTGGAC TTTGACGAGT CGGGCAGTGG AGAGCCCAGT
    GGGGATGATG AGATGGTGAG CGGAATGGAG GAGAGCGGAG AAGAGCCACT TGGTACAACA
    GTAGCTACTA CCACTCTTCC CACAGCTGAG GAGCGATCGT CCTGTGACAA CACAGCGTTC
    GGCTGCTGTC CCGATGGGAA GACGGCCGCC GTCAACTCAG AGGGCACAAA CTGCCCATCC
    ACCATGCGTT TCAGCGGCTT CCTGCACCTG GATAAGGTTG AGGGTCAGGA GGTCTTCTAC
    ACGCCTGAGA TGGAGGACCC CAAATCGGAA CTGTTTGGTG AAACGGCCCG AAGCATCGAG
    AGCGCTATGA ATGAGTTGTT CCGCAAGTCT GATGTCCAGA AAGACTTCCA GAGTGTCCGT
    GTGAGGAATC TATCCCCCAG CAACTCCATA ATAGCACATT TCGAGGCTCA CTTCGACCCA
    GACACAAGGT TCAATGTGGG GGATATTGAG GGAGCCCTTC TCAAACAGCT TAAGGCGTCT
    AAAGACACTG GCATCGTTGT GAAGAAACCA GAGGAAGAGA ACATTCGCAT CAACAACTAT
    GGTCTTTCAA CTGTTCCTTT CTTTACCACC ACAACAACCA CTGTGGCATC TGTCACGACT
    CCCACTACCA CCACCACCAC AATGTCTCCC AGTTCTGCTT ATACCACTCT CCGGCCCATC
    ACTACTAGAC GACCCTTCAC AAGAACCACC ACGCCAGCAC CAGCCACCAC CACTGCACCA
    CATGTGCCCA AGACCACAGT TTCGCTTATG AACAGACTAG GGCCCCACGC TCACAGTCCA
    TGTGACTCTC AGCCCTGCCG ACATGGAGGA ACCTGTGAAG AAGACGACAA TGACTTCACC
    TGTATCTGTC CAGCAGGACG AGGAGGAGCC GTGTGTGAGA AAGTTATAAA GTACTTCATA
    CCATCCTTTG GTGGAAAGTC TTACATGGCG TTCCAGACCA TGAAGGCCTA CCATACTGTA
    CGCATTGCCA TGGAGTTCAG GGCTTCGGAA ATGACTGGGA TATTGCTCTA CAATGGGCAA
    AAAAGCAAAA AGGACTTTCT TTCTCTGACT CTAGTTGAAG GATATGTGGA GCTCAGGTTT
    AACACTGGTT CAGGGACTGC TTCAGTTATC AGTAAAGTCC TGGTAAAGCC GGGTCGCTGG
    CATCAGCTTG TTGTAGTACG TAACAGGCGC AATGCCATGC TGAGCGTGGA CAATGAGCCC
    AGCGTTGAAG GACAAAGTCC TCCAGGAACA GATGGACTCA ACCTGGACAC AGACCTTTTC
    ATCGGCGGAG TGCTTGATGA CATGAGTGCA GACGTAAAGG AGAGGACATC AGTTTCAGCA
    GGTCTGGTGG GCTGCATCCG CATGCTGGAC GTGAACAACC TTGTCTATAA TCTGCAAGAG
    AATGGCGGCA GCGTTCTGTA CGGTTCGGCC ATTGGCGAGT GTGGCAACAA CCCATGCCTG
    CCAAACCCCT GCAAGAACGG AGCGTCCTGT CAGGTCAAAG AAGCCGAGAT TTTCCACTGC
    AAATGTGTCA ACGGTTTCTC TGGCCCGACC TGCGCTGATG CCCACAATCC CTGCGACCCC
    AATAAATGCC ACCCGCCATC ACGGTGCCAG GTCCTTCCAG AGGGAGGTTA CAAATGCGAA
    TGTCCGATGG GACGTGAAGG CAGGCACTGT GAAAAAGTGT CAGACAAGGG AGGCGCTTTC
    ATTCCTTACT TCACTGGAGA TTCTTTTCTG GAGCTCAAAG GCCTCCATAC GTATAACCAG
    GATCTCAGGC AAAAATTCAG CATGACTATC GTTCTACTGG CCAATGACTC CAAAGGCATG
    ATCTTCTACA ATGGTCAGAA GACAGACGGC AAAGGGGATT TCATCTCGCT CTCTCTTAAT
    GACGGCATCC TGGAGTTTAG ATATGACCTG GGCAAAGGGC CAGCTGTCAT TAGGAGTAAA
    GAAAAAATCA AGCTGAACGA GTGGAACACA GTGAATTTGG AACGGGCCAG CAGAAAAGGA
    GAGATTAGCA TTAACGGCAA AGACCCTGTC AGAGGAGAGG CGCCGAATCA GCACACGGAT
    CTCAATCTGA AGGAATCGCT CTTTGTGGGT GGAGCTCCTG ATTTCCGCAA AGTAGCCAGA
    GCTGCTGCGA TCAAAGATGG CTTCAAAGGG GCCATTCAGA AGATTACTTT GATGGGAATT
    CCAATCCTGA AAGCTGATAA TGCCCTTTCG TCCACTGACG TGTCCATGTA TCCTAATCAT
    CCCTGCTCCA AAGACGTTTG TGAGAATGGA GGCCGCTGCA ACCCCCAGCT GAACAGCTAT
    GAGTGCGCCT GCCGCCACGG ATTCTCCGGA CACCACTGCC AGGACACAAT ATTTGAGAAG
    TCCGCTGGGG ACACGGAGGC CATTGCGTTT GATGGTCATA CATTCATCGA ATATCACAAC
    GGAGTCACCA AAAGCGAGAA AGCCCTGCTG GTGAATAAAT TCGAGCTGAG CATCAGGACG
    GAGGCTACGC ATGGTCTGAT TTTGTGGAGC GGAAAGGGAG TTGAGCGCTC TGACTACATC
    GCCCTCGCCA TTGTGGATGG AAGAGTACAG ATGACCTATG ACCTCGGCTC CAAGCCTGTG
    GTGCTGCGCT CCACTGTACG GGTCAACACC AACCACTGGA TACGAATTAA GGCCAGCAGA
    GCGCTCAGAG ATGGCTCTCT GCAGGTTGGT AATGAGGCAG CTGTCACTGG GTCTTCACCC
    TTGGCTGCCA CACAGCTGGA CACAGACGGG GCTCTCTGGT TGGGTGGACT GGAGGAGCTG
    TCTGTGGCTC GACGGCTGCC CAAAGCTTAC AGTACAGGCT TCATTGGCTG CGTCAAAGAC
    GTGATAGTGG ACGGCGTGGA GCTGCATCTG GTGGAGGACG CCTTAAATAC TCCCAAAATA
    TCACACTGTT CTGCCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295142.1
    CDS1644..7424
    Translation

    Target ORF information:

    RefSeq Version XM_009296867.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296867.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGACCAGTT GTCAGTACCC CACGCCGCCC CCAGAGCGGG ACCGCATGTA CCAGCACAAA 
    GTGTCGCTGG TCGTGCGATA CTTCATGATT CCTTGCAACA TCTGTCTCAT ACTGCTGGCC
    ACATCCACGC TCGGCTTTGC TGTACTCCTC TTCCTCAATA ACTATAAACC TGTTCACTTC
    ACACCGGCAC CTCCTCCTGA TGGTTGCAGA GGGAAGTTGT GCGGATTTGG AGCGGTGTGT
    GAGAGAGACC AAGCTGACCC ATCTAAAGGA GAGTGTGTGT GTAAGAAGAT TGTGTGTACA
    TCTGTGGTGG CGCCAGTGTG CGGATCTGAC TCCTCCACCT ATTCCAATGA GTGTGAGCTG
    GAGAAGGCTC AGTGCAATAC ACAGAGACGC ATCAAGGTGA TGCGCAAAGG CCCCTGCGCC
    CTCAAGGACC CGTGCAGTGA GGTAACCTGT AGCTTTGGCA GCACCTGCAT CCAGTCATCG
    GATGGTCTGT CCGCCAAGTG TATGTGTCCT CTGAGCTGTG AAAACGTCCC CAAGCATGTG
    GTGTGTGGCA GTGACGGCCT GGACTACCAA AGCGAATGTG AACTCAACAT GAAGGCCTGC
    GCCACTCAGA AGAACATCCG TGTGCATCAC CAGGGCCGCT GCGATCCTTG CAGTGACACT
    CTGAACAGCT TAAGCACAGC CTGTCGAGTC AACCCAAAGA CCTACCAGCA GATTACCTTT
    GCCCCGGCTG AGTCTTGTCC GCCTGACGAA ATGCCCATCT GTGCCAGCGA CGGACACACC
    TACGAGAGCA TGTGTCAGAT GGAGCGCACT GCCCTGAAGA ACAACCTGGA GCTCAAAAAG
    GTCTCTTTAG GCAGCTGCAA GGAAAAAGGG GGCTGCCCGA ATGGTTGTAA GTTCAACGCC
    ATATGTTTGC TGGATAATGG AGAGTTTCGG TGCTCCTGCG ATCCCATACA ATGCGACGGC
    ACGTACAAGC CCCTGTGCGG AAAGGATGGC AAGACATACC CCAACGACTG CGAGAGGAAA
    CTGCAGGAAT GCCGGACCCA AAAAGACATC CCTGTCAAAC AACAAGGGCC CTGTGATCTT
    AACCTGGAGA GCCCCTGCCT GAAGAAGACC TGCGAGTTTG GAGCCGTGTG CGTGGTGAAG
    AACAGCGAAG CAGTGTGTGA ATGCTCAGAC GCCTGTCCGC AGGACCAAGA CCCTGTTTGC
    GGCAGCGATG GGCACACGTA CAGCAGCTCA TGTCAAATGA AGGCCATGGG ATGCGCTCTT
    CAGAAACAGA TACAGATGCA ACACAAAGGA CCATGTGCAG ATGAGTCCTG CACCAACTGC
    TCGTTTGGCG CGATCTGTGA TGCCCAGACT GGCCGGTGCG TGTGCCCTAA AGGCTGTTTG
    GAAACACGGC AGCCCGTATG CGGCAGCGAC GGCACCACGT ATGACAACGA GTGCAAGCTC
    AACGTCCAAG CCTGCACTAA ACAGCTGGAT CTCAAGGTGG TGGCCCATGG AGAATGCAAA
    ACCTGTGGCA GCACTGTGTG TAGCTGGGGG TCGCAGTGCG TGAACAACAA GTGTGAGTGT
    CAGCAGTGCA CTGGTCAGCC GGTGAAACCC GTGTGTGGCA GCGACGGCAA CACATACAAC
    AACGACTGCG AGCTGAGACT GGCCTCCTGT CAGAAACAGA GGAAGATAGA GGTGGCCAAG
    CCTGGCGTCT GCGATGAAGA CTGTGGTTCA GGAGGCTCCG GCTCGGGATC TGAGACTTGT
    GAACAGGACC GATGTCGCCG ATATGGAGGA ACGTGGGACG ATGACATGGA GGACGAGCGA
    TGTGTTTGTG AACTAAACTG TCAGATTGTG CTGCACAGCC CGGTGTGCGG CTCTGATGGA
    AACACGTACT CCAGCGACTG TGAGCTCAAG AAGACCAGGT GCGAAAAGCA AATAGACCTG
    AGTGTCCAAA GCCAAGGGCC CTGTCCAACT ATCGTATCAC CACCTCCAGA GCTTGTTGCC
    TCCCAGCACT GTAGCCAAAC AGTTTATGGA TGTTGTGAAG ATAACAAGAC CGCTGCCCTG
    GGTGTTGGAC TGGCTGGATG TCCTAGTATG TGTCAGTGCA ATCATTACGG CTCATACGGA
    GGCACATGTG ACCCCTCGAC TGGCCAGTGT TCCTGTAAAC CTGGTGTCGG TGGACTGAAG
    TGCGACCGCT GTGAGCCCGG ATTTTGGAAC TTCCGTGGCA TTGTCACAGA AAACATGAGC
    GGCTGCACAC CGTGCAACTG TGATGGAGTG GGTTCGGTTC GTGATGACTG CGAGCAGATG
    TCAGGCCTGT GCTCCTGTAA GCCTGGAGTT AAGGGCATGA AATGCAACGT GTGCCCTGAT
    GGCAGCAAGA TGGGAGTCAA CGGCTGTGAC AAAGGACCTA AGGCTCCCAA GTCCTGTGCT
    GAGCTTGTGT GCCACTTTGG GTCTTCATGC GTGGAGGTGA ATGGACAGGC TCACTGTGAG
    TGTCCTTCAC CAGATTGTGA TGAGAAAAAC AAGACCAAGG TATGTGGTTC TGATGGGGTC
    ACCTATGCAG ACCGGTGCCA GCTGAAGACC ATCGCATGCC GACAAGATAA AGAGATCAAG
    GTGGAGCATT TAGGCCAGTG TAAAGAATCC ATTGAGCTGA CGGCCAAACC CACCCCCTTC
    CCAACCACCC ACCGCACCAC CCCTCCCACC GCTCACGTCA AGGTCACCAA ACAGCGACAT
    GGCCACCGCA CCACCACTCC GGACAGCTAT GTGGTGGAGG CAATGCCTCC ACCGAGGTTG
    GATGGAGCGC GAAAGTTTTC CAGCCCTTTC ACCACCCACC GTCCCACTAC ACCCTACAAA
    CCACTCCGCA CCTCATCCCC ACCTCTGGAC TTTGACGAGT CGGGCAGTGG AGAGCCCAGT
    GGGGATGATG AGATGGTGAG CGGAATGGAG GAGAGCGGAG AAGAGCCACT TGGTACAACA
    GTAGCTACTA CCACTCTTCC CACAGCTGAG GAGCGATCGT CCTGTGACAA CACAGCGTTC
    GGCTGCTGTC CCGATGGGAA GACGGCCGCC GTCAACTCAG AGGGCACAAA CTGCCCATCC
    ACCATGCGTT TCAGCGGCTT CCTGCACCTG GATAAGGTTG AGGGTCAGGA GGTCTTCTAC
    ACGCCTGAGA TGGAGGACCC CAAATCGGAA CTGTTTGGTG AAACGGCCCG AAGCATCGAG
    AGCGCTATGA ATGAGTTGTT CCGCAAGTCT GATGTCCAGA AAGACTTCCA GAGTGTCCGT
    GTGAGGAATC TATCCCCCAG CAACTCCATA ATAGCACATT TCGAGGCTCA CTTCGACCCA
    GACACAAGGT TCAATGTGGG GGATATTGAG GGAGCCCTTC TCAAACAGCT TAAGGCGTCT
    AAAGACACTG GCATCGTTGT GAAGAAACCA GAGGAAGAGA ACATTCGCAT CAACAACTAT
    GGTCTTTCAA CTGTTCCTTT CTTTACCACC ACAACAACCA CTGTGGCATC TGTCACGACT
    CCCACTACCA CCACCACCAC AATGTCTCCC AGTTCTGCTT ATACCACTCT CCGGCCCATC
    ACTACTAGAC GACCCTTCAC AAGAACCACC ACGCCAGCAC CAGCCACCAC CACTGCACCA
    CATGTGCCCA AGACCACAGT TTCGCTTATG AACAGACTAG GGCCCCACGC TCACAGTCCA
    TGTGACTCTC AGCCCTGCCG ACATGGAGGA ACCTGTGAAG AAGACGACAA TGACTTCACC
    TGTATCTGTC CAGCAGGACG AGGAGGAGCC GTGTGTGAGA AAGTTATAAA GTACTTCATA
    CCATCCTTTG GTGGAAAGTC TTACATGGCG TTCCAGACCA TGAAGGCCTA CCATACTGTA
    CGCATTGCCA TGGAGTTCAG GGCTTCGGAA ATGACTGGGA TATTGCTCTA CAATGGGCAA
    AAAAGCAAAA AGGACTTTCT TTCTCTGACT CTAGTTGAAG GATATGTGGA GCTCAGGTTT
    AACACTGGTT CAGGGACTGC TTCAGTTATC AGTAAAGTCC TGGTAAAGCC GGGTCGCTGG
    CATCAGCTTG TTGTAGTACG TAACAGGCGC AATGCCATGC TGAGCGTGGA CAATGAGCCC
    AGCGTTGAAG GACAAAGTCC TCCAGGAACA GATGGACTCA ACCTGGACAC AGACCTTTTC
    ATCGGCGGAG TGCTTGATGA CATGAGTGCA GACGTAAAGG AGAGGACATC AGTTTCAGCA
    GGTCTGGTGG GCTGCATCCG CATGCTGGAC GTGAACAACC TTGTCTATAA TCTGCAAGAG
    AATGGCGGCA GCGTTCTGTA CGGTTCGGCC ATTGGCGAGT GTGGCAACAA CCCATGCCTG
    CCAAACCCCT GCAAGAACGG AGCGTCCTGT CAGGTCAAAG AAGCCGAGAT TTTCCACTGC
    AAATGTGTCA ACGGTTTCTC TGGCCCGACC TGCGCTGATG CCCACAATCC CTGCGACCCC
    AATAAATGCC ACCCGCCATC ACGGTGCCAG GTCCTTCCAG AGGGAGGTTA CAAATGCGAA
    TGTCCGATGG GACGTGAAGG CAGGCACTGT GAAAAAGTGT CAGACAAGGG AGGCGCTTTC
    ATTCCTTACT TCACTGGAGA TTCTTTTCTG GAGCTCAAAG GCCTCCATAC GTATAACCAG
    GATCTCAGGC AAAAATTCAG CATGACTATC GTTCTACTGG CCAATGACTC CAAAGGCATG
    ATCTTCTACA ATGGTCAGAA GACAGACGGC AAAGGGGATT TCATCTCGCT CTCTCTTAAT
    GACGGCATCC TGGAGTTTAG ATATGACCTG GGCAAAGGGC CAGCTGTCAT TAGGAGTAAA
    GAAAAAATCA AGCTGAACGA GTGGAACACA GTGAATTTGG AACGGGCCAG CAGAAAAGGA
    GAGATTAGCA TTAACGGCAA AGACCCTGTC AGAGGAGAGG CGCCGAATCA GCACACGGAT
    CTCAATCTGA AGGAATCGCT CTTTGTGGGT GGAGCTCCTG ATTTCCGCAA AGTAGCCAGA
    GCTGCTGCGA TCAAAGATGG CTTCAAAGGG GCCATTCAGA AGATTACTTT GATGGGAATT
    CCAATCCTGA AAGCTGATAA TGCCCTTTCG TCCACTGACG TGTCCATGTA TCCTAATCAT
    CCCTGCTCCA AAGACGTTTG TGAGAATGGA GGCCGCTGCA ACCCCCAGCT GAACAGCTAT
    GAGTGCGCCT GCCGCCACGG ATTCTCCGGA CACCACTGCC AGGACACAAT ATTTGAGAAG
    TCCGCTGGGG ACACGGAGGC CATTGCGTTT GATGGTCATA CATTCATCGA ATATCACAAC
    GGAGTCACCA AAAGCGAGAA AGCCCTGCTG GTGAATAAAT TCGAGCTGAG CATCAGGACG
    GAGGCTACGC ATGGTCTGAT TTTGTGGAGC GGAAAGGGAG TTGAGCGCTC TGACTACATC
    GCCCTCGCCA TTGTGGATGG AAGAGTACAG ATGACCTATG ACCTCGGCTC CAAGCCTGTG
    GTGCTGCGCT CCACTGTACG GGTCAACACC AACCACTGGA TACGAATTAA GGCCAGCAGA
    GCGCTCAGAG ATGGCTCTCT GCAGGTTGGT AATGAGGCAG CTGTCACTGG GTCTTCACCC
    TTGGCTGCCA CACAGCTGGA CACAGACGGG GCTCTCTGGT TGGGTGGACT GGAGGAGCTG
    TCTGTGGCTC GACGGCTGCC CAAAGCTTAC AGTACAGGCT TCATTGGCTG CGTCAAAGAC
    GTGATAGTGG ACGGCGTGGA GCTGCATCTG GTGGAGGACG CCTTAAATAC TCCCAAAATA
    TCACACTGTT CTGCCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151475
    Clone ID Related Accession (Same CDS sequence) XM_021469829.1
    Accession Version XM_021469829.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product agrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAAAT CACGCAAGAA TCAGCACACG GATCTCAATC TGAAGGAATC GCTCTTTGTG
    GGTGGAGCTC CTGATTTCCG CAAAGTAGCC AGAGCTGCTG CGATCAAAGA TGGCTTCAAA
    GGGGCCATTC AGAAGATTAC TTTGATGGGA ATTCCAATCC TGAAAGCTGA TAATGCCCTT
    TCGTCCACTG ACGTGTCCAT GTATCCTAAT CATCCCTGCT CCAAAGACGT TTGTGAGAAT
    GGAGGCCGCT GCAACCCCCA GCTGAACAGC TATGAGTGCG CCTGCCGCCA CGGATTCTCC
    GGACACCACT GCCAGGACAC AATATTTGAG AAGTCCGCTG GGGACACGGA GGCCATTGCG
    TTTGATGGTC ATACATTCAT CGAATATCAC AACGGAGTCA CCAAAAGCCA ACTGACCAAT
    GAGATCCCAG ACCCTGAGTC ACTGGAGAAC CCATCTGACC AGAGCGAGAA AGCCCTGCTG
    GTGAATAAAT TCGAGCTGAG CATCAGGACG GAGGCTACGC ATGGTCTGAT TTTGTGGAGC
    GGAAAGGGAG TTGAGCGCTC TGACTACATC GCCCTCGCCA TTGTGGATGG AAGAGTACAG
    ATGACCTATG ACCTCGGCTC CAAGCCTGTG GTGCTGCGCT CCACTGTACG GGTCAACACC
    AACCACTGGA TACGAATTAA GGCCAGCAGA GCGCTCAGAG ATGGCTCTCT GCAGGTTGGT
    AATGAGGCAG CTGTCACTGG GTCTTCACCC TTGGCTGCCA CACAGCTGGA CACAGACGGG
    GCTCTCTGGT TGGGTGGACT GGAGGAGCTG TCTGTGGCTC GACGGCTGCC CAAAGCTTAC
    AGTACAGGCT TCATTGGCTG CGTCAAAGAC GTGATAGTGG ACGGCGTGGA GCTGCATCTG
    GTGGAGGACG CCTTAAATAC TCCCAAAATA TCACACTGTT CTGCCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325504.1
    CDS266..6436
    Translation

    Target ORF information:

    RefSeq Version XM_021469829.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469829.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAAAT CACGCAAGAA TCAGCACACG GATCTCAATC TGAAGGAATC GCTCTTTGTG
    GGTGGAGCTC CTGATTTCCG CAAAGTAGCC AGAGCTGCTG CGATCAAAGA TGGCTTCAAA
    GGGGCCATTC AGAAGATTAC TTTGATGGGA ATTCCAATCC TGAAAGCTGA TAATGCCCTT
    TCGTCCACTG ACGTGTCCAT GTATCCTAAT CATCCCTGCT CCAAAGACGT TTGTGAGAAT
    GGAGGCCGCT GCAACCCCCA GCTGAACAGC TATGAGTGCG CCTGCCGCCA CGGATTCTCC
    GGACACCACT GCCAGGACAC AATATTTGAG AAGTCCGCTG GGGACACGGA GGCCATTGCG
    TTTGATGGTC ATACATTCAT CGAATATCAC AACGGAGTCA CCAAAAGCCA ACTGACCAAT
    GAGATCCCAG ACCCTGAGTC ACTGGAGAAC CCATCTGACC AGAGCGAGAA AGCCCTGCTG
    GTGAATAAAT TCGAGCTGAG CATCAGGACG GAGGCTACGC ATGGTCTGAT TTTGTGGAGC
    GGAAAGGGAG TTGAGCGCTC TGACTACATC GCCCTCGCCA TTGTGGATGG AAGAGTACAG
    ATGACCTATG ACCTCGGCTC CAAGCCTGTG GTGCTGCGCT CCACTGTACG GGTCAACACC
    AACCACTGGA TACGAATTAA GGCCAGCAGA GCGCTCAGAG ATGGCTCTCT GCAGGTTGGT
    AATGAGGCAG CTGTCACTGG GTCTTCACCC TTGGCTGCCA CACAGCTGGA CACAGACGGG
    GCTCTCTGGT TGGGTGGACT GGAGGAGCTG TCTGTGGCTC GACGGCTGCC CAAAGCTTAC
    AGTACAGGCT TCATTGGCTG CGTCAAAGAC GTGATAGTGG ACGGCGTGGA GCTGCATCTG
    GTGGAGGACG CCTTAAATAC TCCCAAAATA TCACACTGTT CTGCCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151476
    Clone ID Related Accession (Same CDS sequence) XM_021469830.1
    Accession Version XM_021469830.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6168bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product agrin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGAT
    GAGTCCTGCA CCAACTGCTC GTTTGGCGCG ATCTGTGATG CCCAGACTGG CCGGTGCGTG
    TGCCCTAAAG GCTGTTTGGA AACACGGCAG CCCGTATGCG GCAGCGACGG CACCACGTAT
    GACAACGAGT GCAAGCTCAA CGTCCAAGCC TGCACTAAAC AGCTGGATCT CAAGGTGGTG
    GCCCATGGAG AATGCAAAAC CTGTGGCAGC ACTGTGTGTA GCTGGGGGTC GCAGTGCGTG
    AACAACAAGT GTGAGTGTCA GCAGTGCACT GGTCAGCCGG TGAAACCCGT GTGTGGCAGC
    GACGGCAACA CATACAACAA CGACTGCGAG CTGAGACTGG CCTCCTGTCA GAAACAGAGG
    AAGATAGAGG TGGCCAAGCC TGGCGTCTGC GATGAAGACT GTGGTTCAGG AGGCTCCGGC
    TCGGGATCTG AGACTTGTGA ACAGGACCGA TGTCGCCGAT ATGGAGGAAC GTGGGACGAT
    GACATGGAGG ACGAGCGATG TGTTTGTGAA CTAAACTGTC AGATTGTGCT GCACAGCCCG
    GTGTGCGGCT CTGATGGAAA CACGTACTCC AGCGACTGTG AGCTCAAGAA GACCAGGTGC
    GAAAAGCAAA TAGACCTGAG TGTCCAAAGC CAAGGGCCCT GTCCAACTAT CGTATCACCA
    CCTCCAGAGC TTGTTGCCTC CCAGCACTGT AGCCAAACAG TTTATGGATG TTGTGAAGAT
    AACAAGACCG CTGCCCTGGG TGTTGGACTG GCTGGATGTC CTAGTATGTG TCAGTGCAAT
    CATTACGGCT CATACGGAGG CACATGTGAC CCCTCGACTG GCCAGTGTTC CTGTAAACCT
    GGTGTCGGTG GACTGAAGTG CGACCGCTGT GAGCCCGGAT TTTGGAACTT CCGTGGCATT
    GTCACAGAAA ACATGAGCGG CTGCACACCG TGCAACTGTG ATGGAGTGGG TTCGGTTCGT
    GATGACTGCG AGCAGATGTC AGGCCTGTGC TCCTGTAAGC CTGGAGTTAA GGGCATGAAA
    TGCAACGTGT GCCCTGATGG CAGCAAGATG GGAGTCAACG GCTGTGACAA AGGACCTAAG
    GCTCCCAAGT CCTGTGCTGA GCTTGTGTGC CACTTTGGGT CTTCATGCGT GGAGGTGAAT
    GGACAGGCTC ACTGTGAGTG TCCTTCACCA GATTGTGATG AGAAAAACAA GACCAAGGTA
    TGTGGTTCTG ATGGGGTCAC CTATGCAGAC CGGTGCCAGC TGAAGACCAT CGCATGCCGA
    CAAGATAAAG AGATCAAGGT GGAGCATTTA GGCCAGTGTA AAGAATCCAT TGAGCTGACG
    GCCAAACCCA CCCCCTTCCC AACCACCCAC CGCACCACCC CTCCCACCGC TCACGTCAAG
    GTCACCAAAC AGCGACATGG CCACCGCACC ACCACTCCGG ACAGCTATGT GGTGGAGGCA
    ATGCCTCCAC CGAGGTTGGA TGGAGCGCGA AAGTTTTCCA GCCCTTTCAC CACCCACCGT
    CCCACTACAC CCTACAAACC ACTCCGCACC TCATCCCCAC CTCTGGACTT TGACGAGTCG
    GGCAGTGGAG AGCCCAGTGG GGATGATGAG ATGGTGAGCG GAATGGAGGA GAGCGGAGAA
    GAGCCACTTG GTACAACAGT AGCTACTACC ACTCTTCCCA CAGCTGAGGA GCGATCGTCC
    TGTGACAACA CAGCGTTCGG CTGCTGTCCC GATGGGAAGA CGGCCGCCGT CAACTCAGAG
    GGCACAAACT GCCCATCCAC CATGCGTTTC AGCGGCTTCC TGCACCTGGA TAAGGTTGAG
    GGTCAGGAGG TCTTCTACAC GCCTGAGATG GAGGACCCCA AATCGGAACT GTTTGGTGAA
    ACGGCCCGAA GCATCGAGAG CGCTATGAAT GAGTTGTTCC GCAAGTCTGA TGTCCAGAAA
    GACTTCCAGA GTGTCCGTGT GAGGAATCTA TCCCCCAGCA ACTCCATAAT AGCACATTTC
    GAGGCTCACT TCGACCCAGA CACAAGGTTC AATGTGGGGG ATATTGAGGG AGCCCTTCTC
    AAACAGCTTA AGGCGTCTAA AGACACTGGC ATCGTTGTGA AGAAACCAGA GGAAGAGAAC
    ATTCGCATCA ACAACTATGG TCTTTCAACT GTTCCTTTCT TTACCACCAC AACAACCACT
    GTGGCATCTG TCACGACTCC CACTACCACC ACCACCACAA TGTCTCCCAG TTCTGCTTAT
    ACCACTCTCC GGCCCATCAC TACTAGACGA CCCTTCACAA GAACCACCAC GCCAGCACCA
    GCCACCACCA CTGCACCACA TGTGCCCAAG ACCACAGTTT CGCTTATGAA CAGACTAGGG
    CCCCACGCTC ACAGTCCATG TGACTCTCAG CCCTGCCGAC ATGGAGGAAC CTGTGAAGAA
    GACGACAATG ACTTCACCTG TATCTGTCCA GCAGGACGAG GAGGAGCCGT GTGTGAGAAA
    GTTATAAAGT ACTTCATACC ATCCTTTGGT GGAAAGTCTT ACATGGCGTT CCAGACCATG
    AAGGCCTACC ATACTGTACG CATTGCCATG GAGTTCAGGG CTTCGGAAAT GACTGGGATA
    TTGCTCTACA ATGGGCAAAA AAGCAAAAAG GACTTTCTTT CTCTGACTCT AGTTGAAGGA
    TATGTGGAGC TCAGGTTTAA CACTGGTTCA GGGACTGCTT CAGTTATCAG TAAAGTCCTG
    GTAAAGCCGG GTCGCTGGCA TCAGCTTGTT GTAGTACGTA ACAGGCGCAA TGCCATGCTG
    AGCGTGGACA ATGAGCCCAG CGTTGAAGGA CAAAGTCCTC CAGGAACAGA TGGACTCAAC
    CTGGACACAG ACCTTTTCAT CGGCGGAGTG CTTGATGACA TGAGTGCAGA CGTAAAGGAG
    AGGACATCAG TTTCAGCAGG TCTGGTGGGC TGCATCCGCA TGCTGGACGT GAACAACCTT
    GTCTATAATC TGCAAGAGAA TGGCGGCAGC GTTCTGTACG GTTCGGCCAT TGGCGAGTGT
    GGCAACAACC CATGCCTGCC AAACCCCTGC AAGAACGGAG CGTCCTGTCA GGTCAAAGAA
    GCCGAGATTT TCCACTGCAA ATGTGTCAAC GGTTTCTCTG GCCCGACCTG CGCTGATGCC
    CACAATCCCT GCGACCCCAA TAAATGCCAC CCGCCATCAC GGTGCCAGGT CCTTCCAGAG
    GGAGGTTACA AATGCGAATG TCCGATGGGA CGTGAAGGCA GGCACTGTGA AAAAGTGTCA
    GACAAGGGAG GCGCTTTCAT TCCTTACTTC ACTGGAGATT CTTTTCTGGA GCTCAAAGGC
    CTCCATACGT ATAACCAGGA TCTCAGGCAA AAATTCAGCA TGACTATCGT TCTACTGGCC
    AATGACTCCA AAGGCATGAT CTTCTACAAT GGTCAGAAGA CAGACGGCAA AGGGGATTTC
    ATCTCGCTCT CTCTTAATGA CGGCATCCTG GAGTTTAGAT ATGACCTGGG CAAAGGGCCA
    GCTGTCATTA GGAGTAAAGA AAAAATCAAG CTGAACGAGT GGAACACAGT GAATTTGGAA
    CGGGCCAGCA GAAAAGGAGA GATTAGCATT AACGGCAAAG ACCCTGTCAG AGGAGAGGCG
    CCGAAATCAC GCAAGAATCA GCACACGGAT CTCAATCTGA AGGAATCGCT CTTTGTGGGT
    GGAGCTCCTG ATTTCCGCAA AGTAGCCAGA GCTGCTGCGA TCAAAGATGG CTTCAAAGGG
    GCCATTCAGA AGATTACTTT GATGGGAATT CCAATCCTGA AAGCTGATAA TGCCCTTTCG
    TCCACTGACG TGTCCATGTA TCCTAATCAT CCCTGCTCCA AAGACGTTTG TGAGAATGGA
    GGCCGCTGCA ACCCCCAGCT GAACAGCTAT GAGTGCGCCT GCCGCCACGG ATTCTCCGGA
    CACCACTGCC AGGACACAAT ATTTGAGAAG TCCGCTGGGG ACACGGAGGC CATTGCGTTT
    GATGGTCATA CATTCATCGA ATATCACAAC GGAGTCACCA AAAGCCAACT GACCAATGAG
    ATCCCAGACC CTGAGTCACT GGAGAACCCA TCTGACCAGA GCGAGAAAGC CCTGCTGGTG
    AATAAATTCG AGCTGAGCAT CAGGACGGAG GCTACGCATG GTCTGATTTT GTGGAGCGGA
    AAGGGAGTTG AGCGCTCTGA CTACATCGCC CTCGCCATTG TGGATGGAAG AGTACAGATG
    ACCTATGACC TCGGCTCCAA GCCTGTGGTG CTGCGCTCCA CTGTACGGGT CAACACCAAC
    CACTGGATAC GAATTAAGGC CAGCAGAGCG CTCAGAGATG GCTCTCTGCA GGTTGGTAAT
    GAGGCAGCTG TCACTGGGTC TTCACCCTTG GCTGCCACAC AGCTGGACAC AGACGGGGCT
    CTCTGGTTGG GTGGACTGGA GGAGCTGTCT GTGGCTCGAC GGCTGCCCAA AGCTTACAGT
    ACAGGCTTCA TTGGCTGCGT CAAAGACGTG ATAGTGGACG GCGTGGAGCT GCATCTGGTG
    GAGGACGCCT TAAATACTCC CAAAATATCA CACTGTTCTG CCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325505.1
    CDS266..6433
    Translation

    Target ORF information:

    RefSeq Version XM_021469830.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469830.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGAT
    GAGTCCTGCA CCAACTGCTC GTTTGGCGCG ATCTGTGATG CCCAGACTGG CCGGTGCGTG
    TGCCCTAAAG GCTGTTTGGA AACACGGCAG CCCGTATGCG GCAGCGACGG CACCACGTAT
    GACAACGAGT GCAAGCTCAA CGTCCAAGCC TGCACTAAAC AGCTGGATCT CAAGGTGGTG
    GCCCATGGAG AATGCAAAAC CTGTGGCAGC ACTGTGTGTA GCTGGGGGTC GCAGTGCGTG
    AACAACAAGT GTGAGTGTCA GCAGTGCACT GGTCAGCCGG TGAAACCCGT GTGTGGCAGC
    GACGGCAACA CATACAACAA CGACTGCGAG CTGAGACTGG CCTCCTGTCA GAAACAGAGG
    AAGATAGAGG TGGCCAAGCC TGGCGTCTGC GATGAAGACT GTGGTTCAGG AGGCTCCGGC
    TCGGGATCTG AGACTTGTGA ACAGGACCGA TGTCGCCGAT ATGGAGGAAC GTGGGACGAT
    GACATGGAGG ACGAGCGATG TGTTTGTGAA CTAAACTGTC AGATTGTGCT GCACAGCCCG
    GTGTGCGGCT CTGATGGAAA CACGTACTCC AGCGACTGTG AGCTCAAGAA GACCAGGTGC
    GAAAAGCAAA TAGACCTGAG TGTCCAAAGC CAAGGGCCCT GTCCAACTAT CGTATCACCA
    CCTCCAGAGC TTGTTGCCTC CCAGCACTGT AGCCAAACAG TTTATGGATG TTGTGAAGAT
    AACAAGACCG CTGCCCTGGG TGTTGGACTG GCTGGATGTC CTAGTATGTG TCAGTGCAAT
    CATTACGGCT CATACGGAGG CACATGTGAC CCCTCGACTG GCCAGTGTTC CTGTAAACCT
    GGTGTCGGTG GACTGAAGTG CGACCGCTGT GAGCCCGGAT TTTGGAACTT CCGTGGCATT
    GTCACAGAAA ACATGAGCGG CTGCACACCG TGCAACTGTG ATGGAGTGGG TTCGGTTCGT
    GATGACTGCG AGCAGATGTC AGGCCTGTGC TCCTGTAAGC CTGGAGTTAA GGGCATGAAA
    TGCAACGTGT GCCCTGATGG CAGCAAGATG GGAGTCAACG GCTGTGACAA AGGACCTAAG
    GCTCCCAAGT CCTGTGCTGA GCTTGTGTGC CACTTTGGGT CTTCATGCGT GGAGGTGAAT
    GGACAGGCTC ACTGTGAGTG TCCTTCACCA GATTGTGATG AGAAAAACAA GACCAAGGTA
    TGTGGTTCTG ATGGGGTCAC CTATGCAGAC CGGTGCCAGC TGAAGACCAT CGCATGCCGA
    CAAGATAAAG AGATCAAGGT GGAGCATTTA GGCCAGTGTA AAGAATCCAT TGAGCTGACG
    GCCAAACCCA CCCCCTTCCC AACCACCCAC CGCACCACCC CTCCCACCGC TCACGTCAAG
    GTCACCAAAC AGCGACATGG CCACCGCACC ACCACTCCGG ACAGCTATGT GGTGGAGGCA
    ATGCCTCCAC CGAGGTTGGA TGGAGCGCGA AAGTTTTCCA GCCCTTTCAC CACCCACCGT
    CCCACTACAC CCTACAAACC ACTCCGCACC TCATCCCCAC CTCTGGACTT TGACGAGTCG
    GGCAGTGGAG AGCCCAGTGG GGATGATGAG ATGGTGAGCG GAATGGAGGA GAGCGGAGAA
    GAGCCACTTG GTACAACAGT AGCTACTACC ACTCTTCCCA CAGCTGAGGA GCGATCGTCC
    TGTGACAACA CAGCGTTCGG CTGCTGTCCC GATGGGAAGA CGGCCGCCGT CAACTCAGAG
    GGCACAAACT GCCCATCCAC CATGCGTTTC AGCGGCTTCC TGCACCTGGA TAAGGTTGAG
    GGTCAGGAGG TCTTCTACAC GCCTGAGATG GAGGACCCCA AATCGGAACT GTTTGGTGAA
    ACGGCCCGAA GCATCGAGAG CGCTATGAAT GAGTTGTTCC GCAAGTCTGA TGTCCAGAAA
    GACTTCCAGA GTGTCCGTGT GAGGAATCTA TCCCCCAGCA ACTCCATAAT AGCACATTTC
    GAGGCTCACT TCGACCCAGA CACAAGGTTC AATGTGGGGG ATATTGAGGG AGCCCTTCTC
    AAACAGCTTA AGGCGTCTAA AGACACTGGC ATCGTTGTGA AGAAACCAGA GGAAGAGAAC
    ATTCGCATCA ACAACTATGG TCTTTCAACT GTTCCTTTCT TTACCACCAC AACAACCACT
    GTGGCATCTG TCACGACTCC CACTACCACC ACCACCACAA TGTCTCCCAG TTCTGCTTAT
    ACCACTCTCC GGCCCATCAC TACTAGACGA CCCTTCACAA GAACCACCAC GCCAGCACCA
    GCCACCACCA CTGCACCACA TGTGCCCAAG ACCACAGTTT CGCTTATGAA CAGACTAGGG
    CCCCACGCTC ACAGTCCATG TGACTCTCAG CCCTGCCGAC ATGGAGGAAC CTGTGAAGAA
    GACGACAATG ACTTCACCTG TATCTGTCCA GCAGGACGAG GAGGAGCCGT GTGTGAGAAA
    GTTATAAAGT ACTTCATACC ATCCTTTGGT GGAAAGTCTT ACATGGCGTT CCAGACCATG
    AAGGCCTACC ATACTGTACG CATTGCCATG GAGTTCAGGG CTTCGGAAAT GACTGGGATA
    TTGCTCTACA ATGGGCAAAA AAGCAAAAAG GACTTTCTTT CTCTGACTCT AGTTGAAGGA
    TATGTGGAGC TCAGGTTTAA CACTGGTTCA GGGACTGCTT CAGTTATCAG TAAAGTCCTG
    GTAAAGCCGG GTCGCTGGCA TCAGCTTGTT GTAGTACGTA ACAGGCGCAA TGCCATGCTG
    AGCGTGGACA ATGAGCCCAG CGTTGAAGGA CAAAGTCCTC CAGGAACAGA TGGACTCAAC
    CTGGACACAG ACCTTTTCAT CGGCGGAGTG CTTGATGACA TGAGTGCAGA CGTAAAGGAG
    AGGACATCAG TTTCAGCAGG TCTGGTGGGC TGCATCCGCA TGCTGGACGT GAACAACCTT
    GTCTATAATC TGCAAGAGAA TGGCGGCAGC GTTCTGTACG GTTCGGCCAT TGGCGAGTGT
    GGCAACAACC CATGCCTGCC AAACCCCTGC AAGAACGGAG CGTCCTGTCA GGTCAAAGAA
    GCCGAGATTT TCCACTGCAA ATGTGTCAAC GGTTTCTCTG GCCCGACCTG CGCTGATGCC
    CACAATCCCT GCGACCCCAA TAAATGCCAC CCGCCATCAC GGTGCCAGGT CCTTCCAGAG
    GGAGGTTACA AATGCGAATG TCCGATGGGA CGTGAAGGCA GGCACTGTGA AAAAGTGTCA
    GACAAGGGAG GCGCTTTCAT TCCTTACTTC ACTGGAGATT CTTTTCTGGA GCTCAAAGGC
    CTCCATACGT ATAACCAGGA TCTCAGGCAA AAATTCAGCA TGACTATCGT TCTACTGGCC
    AATGACTCCA AAGGCATGAT CTTCTACAAT GGTCAGAAGA CAGACGGCAA AGGGGATTTC
    ATCTCGCTCT CTCTTAATGA CGGCATCCTG GAGTTTAGAT ATGACCTGGG CAAAGGGCCA
    GCTGTCATTA GGAGTAAAGA AAAAATCAAG CTGAACGAGT GGAACACAGT GAATTTGGAA
    CGGGCCAGCA GAAAAGGAGA GATTAGCATT AACGGCAAAG ACCCTGTCAG AGGAGAGGCG
    CCGAAATCAC GCAAGAATCA GCACACGGAT CTCAATCTGA AGGAATCGCT CTTTGTGGGT
    GGAGCTCCTG ATTTCCGCAA AGTAGCCAGA GCTGCTGCGA TCAAAGATGG CTTCAAAGGG
    GCCATTCAGA AGATTACTTT GATGGGAATT CCAATCCTGA AAGCTGATAA TGCCCTTTCG
    TCCACTGACG TGTCCATGTA TCCTAATCAT CCCTGCTCCA AAGACGTTTG TGAGAATGGA
    GGCCGCTGCA ACCCCCAGCT GAACAGCTAT GAGTGCGCCT GCCGCCACGG ATTCTCCGGA
    CACCACTGCC AGGACACAAT ATTTGAGAAG TCCGCTGGGG ACACGGAGGC CATTGCGTTT
    GATGGTCATA CATTCATCGA ATATCACAAC GGAGTCACCA AAAGCCAACT GACCAATGAG
    ATCCCAGACC CTGAGTCACT GGAGAACCCA TCTGACCAGA GCGAGAAAGC CCTGCTGGTG
    AATAAATTCG AGCTGAGCAT CAGGACGGAG GCTACGCATG GTCTGATTTT GTGGAGCGGA
    AAGGGAGTTG AGCGCTCTGA CTACATCGCC CTCGCCATTG TGGATGGAAG AGTACAGATG
    ACCTATGACC TCGGCTCCAA GCCTGTGGTG CTGCGCTCCA CTGTACGGGT CAACACCAAC
    CACTGGATAC GAATTAAGGC CAGCAGAGCG CTCAGAGATG GCTCTCTGCA GGTTGGTAAT
    GAGGCAGCTG TCACTGGGTC TTCACCCTTG GCTGCCACAC AGCTGGACAC AGACGGGGCT
    CTCTGGTTGG GTGGACTGGA GGAGCTGTCT GTGGCTCGAC GGCTGCCCAA AGCTTACAGT
    ACAGGCTTCA TTGGCTGCGT CAAAGACGTG ATAGTGGACG GCGTGGAGCT GCATCTGGTG
    GAGGACGCCT TAAATACTCC CAAAATATCA CACTGTTCTG CCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151477
    Clone ID Related Accession (Same CDS sequence) XM_021469831.1
    Accession Version XM_021469831.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6159bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product agrin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAATC AGCACACGGA TCTCAATCTG AAGGAATCGC TCTTTGTGGG TGGAGCTCCT
    GATTTCCGCA AAGTAGCCAG AGCTGCTGCG ATCAAAGATG GCTTCAAAGG GGCCATTCAG
    AAGATTACTT TGATGGGAAT TCCAATCCTG AAAGCTGATA ATGCCCTTTC GTCCACTGAC
    GTGTCCATGT ATCCTAATCA TCCCTGCTCC AAAGACGTTT GTGAGAATGG AGGCCGCTGC
    AACCCCCAGC TGAACAGCTA TGAGTGCGCC TGCCGCCACG GATTCTCCGG ACACCACTGC
    CAGGACACAA TATTTGAGAA GTCCGCTGGG GACACGGAGG CCATTGCGTT TGATGGTCAT
    ACATTCATCG AATATCACAA CGGAGTCACC AAAAGCCAAC TGACCAATGA GATCCCAGAC
    CCTGAGTCAC TGGAGAACCC ATCTGACCAG AGCGAGAAAG CCCTGCTGGT GAATAAATTC
    GAGCTGAGCA TCAGGACGGA GGCTACGCAT GGTCTGATTT TGTGGAGCGG AAAGGGAGTT
    GAGCGCTCTG ACTACATCGC CCTCGCCATT GTGGATGGAA GAGTACAGAT GACCTATGAC
    CTCGGCTCCA AGCCTGTGGT GCTGCGCTCC ACTGTACGGG TCAACACCAA CCACTGGATA
    CGAATTAAGG CCAGCAGAGC GCTCAGAGAT GGCTCTCTGC AGGTTGGTAA TGAGGCAGCT
    GTCACTGGGT CTTCACCCTT GGCTGCCACA CAGCTGGACA CAGACGGGGC TCTCTGGTTG
    GGTGGACTGG AGGAGCTGTC TGTGGCTCGA CGGCTGCCCA AAGCTTACAG TACAGGCTTC
    ATTGGCTGCG TCAAAGACGT GATAGTGGAC GGCGTGGAGC TGCATCTGGT GGAGGACGCC
    TTAAATACTC CCAAAATATC ACACTGTTCT GCCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325506.1
    CDS266..6424
    Translation

    Target ORF information:

    RefSeq Version XM_021469831.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469831.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAATC AGCACACGGA TCTCAATCTG AAGGAATCGC TCTTTGTGGG TGGAGCTCCT
    GATTTCCGCA AAGTAGCCAG AGCTGCTGCG ATCAAAGATG GCTTCAAAGG GGCCATTCAG
    AAGATTACTT TGATGGGAAT TCCAATCCTG AAAGCTGATA ATGCCCTTTC GTCCACTGAC
    GTGTCCATGT ATCCTAATCA TCCCTGCTCC AAAGACGTTT GTGAGAATGG AGGCCGCTGC
    AACCCCCAGC TGAACAGCTA TGAGTGCGCC TGCCGCCACG GATTCTCCGG ACACCACTGC
    CAGGACACAA TATTTGAGAA GTCCGCTGGG GACACGGAGG CCATTGCGTT TGATGGTCAT
    ACATTCATCG AATATCACAA CGGAGTCACC AAAAGCCAAC TGACCAATGA GATCCCAGAC
    CCTGAGTCAC TGGAGAACCC ATCTGACCAG AGCGAGAAAG CCCTGCTGGT GAATAAATTC
    GAGCTGAGCA TCAGGACGGA GGCTACGCAT GGTCTGATTT TGTGGAGCGG AAAGGGAGTT
    GAGCGCTCTG ACTACATCGC CCTCGCCATT GTGGATGGAA GAGTACAGAT GACCTATGAC
    CTCGGCTCCA AGCCTGTGGT GCTGCGCTCC ACTGTACGGG TCAACACCAA CCACTGGATA
    CGAATTAAGG CCAGCAGAGC GCTCAGAGAT GGCTCTCTGC AGGTTGGTAA TGAGGCAGCT
    GTCACTGGGT CTTCACCCTT GGCTGCCACA CAGCTGGACA CAGACGGGGC TCTCTGGTTG
    GGTGGACTGG AGGAGCTGTC TGTGGCTCGA CGGCTGCCCA AAGCTTACAG TACAGGCTTC
    ATTGGCTGCG TCAAAGACGT GATAGTGGAC GGCGTGGAGC TGCATCTGGT GGAGGACGCC
    TTAAATACTC CCAAAATATC ACACTGTTCT GCCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151478
    Clone ID Related Accession (Same CDS sequence) XM_021469832.1
    Accession Version XM_021469832.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product agrin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATAAACC TGTTCACTTC
    ACACCGGCAC CTCCTCCTGA TGGTTGCAGA GGGAAGTTGT GCGGATTTGG AGCGGTGTGT
    GAGAGAGACC AAGCTGACCC ATCTAAAGGA GAGTGTGTGT GTAAGAAGAT TGTGTGTACA
    TCTGTGGTGG CGCCAGTGTG CGGATCTGAC TCCTCCACCT ATTCCAATGA GTGTGAGCTG
    GAGAAGGCTC AGTGCAATAC ACAGAGACGC ATCAAGGTGA TGCGCAAAGG CCCCTGCGCC
    CTCAAGGACC CGTGCAGTGA GGTAACCTGT AGCTTTGGCA GCACCTGCAT CCAGTCATCG
    GATGGTCTGT CCGCCAAGTG TATGTGTCCT CTGAGCTGTG AAAACGTCCC CAAGCATGTG
    GTGTGTGGCA GTGACGGCCT GGACTACCAA AGCGAATGTG AACTCAACAT GAAGGCCTGC
    GCCACTCAGA AGAACATCCG TGTGCATCAC CAGGGCCGCT GCGATCCTTG CAGTGACACT
    CTGAACAGCT TAAGCACAGC CTGTCGAGTC AACCCAAAGA CCTACCAGCA GATTACCTTT
    GCCCCGGCTG AGTCTTGTCC GCCTGACGAA ATGCCCATCT GTGCCAGCGA CGGACACACC
    TACGAGAGCA TGTGTCAGAT GGAGCGCACT GCCCTGAAGA ACAACCTGGA GCTCAAAAAG
    GTCTCTTTAG GCAGCTGCAA GGAAAAAGGG GGCTGCCCGA ATGGTTGTAA GTTCAACGCC
    ATATGTTTGC TGGATAATGG AGAGTTTCGG TGCTCCTGCG ATCCCATACA ATGCGACGGC
    ACGTACAAGC CCCTGTGCGG AAAGGATGGC AAGACATACC CCAACGACTG CGAGAGGAAA
    CTGCAGGAAT GCCGGACCCA AAAAGACATC CCTGTCAAAC AACAAGGGCC CTGTGATCTT
    AACCTGGAGA GCCCCTGCCT GAAGAAGACC TGCGAGTTTG GAGCCGTGTG CGTGGTGAAG
    AACAGCGAAG CAGTGTGTGA ATGCTCAGAC GCCTGTCCGC AGGACCAAGA CCCTGTTTGC
    GGCAGCGATG GGCACACGTA CAGCAGCTCA TGTCAAATGA AGGCCATGGG ATGCGCTCTT
    CAGAAACAGA TACAGATGCA ACACAAAGGA CCATGTGCAG ATGAGTCCTG CACCAACTGC
    TCGTTTGGCG CGATCTGTGA TGCCCAGACT GGCCGGTGCG TGTGCCCTAA AGGCTGTTTG
    GAAACACGGC AGCCCGTATG CGGCAGCGAC GGCACCACGT ATGACAACGA GTGCAAGCTC
    AACGTCCAAG CCTGCACTAA ACAGCTGGAT CTCAAGGTGG TGGCCCATGG AGAATGCAAA
    ACCTGTGGCA GCACTGTGTG TAGCTGGGGG TCGCAGTGCG TGAACAACAA GTGTGAGTGT
    CAGCAGTGCA CTGGTCAGCC GGTGAAACCC GTGTGTGGCA GCGACGGCAA CACATACAAC
    AACGACTGCG AGCTGAGACT GGCCTCCTGT CAGAAACAGA GGAAGATAGA GGTGGCCAAG
    CCTGGCGTCT GCGATGAAGA CTGTGGTTCA GGAGGCTCCG GCTCGGGATC TGAGACTTGT
    GAACAGGACC GATGTCGCCG ATATGGAGGA ACGTGGGACG ATGACATGGA GGACGAGCGA
    TGTGTTTGTG AACTAAACTG TCAGATTGTG CTGCACAGCC CGGTGTGCGG CTCTGATGGA
    AACACGTACT CCAGCGACTG TGAGCTCAAG AAGACCAGGT GCGAAAAGCA AATAGACCTG
    AGTGTCCAAA GCCAAGGGCC CTGTCCAACT ATCGTATCAC CACCTCCAGA GCTTGTTGCC
    TCCCAGCACT GTAGCCAAAC AGTTTATGGA TGTTGTGAAG ATAACAAGAC CGCTGCCCTG
    GGTGTTGGAC TGGCTGGATG TCCTAGTATG TGTCAGTGCA ATCATTACGG CTCATACGGA
    GGCACATGTG ACCCCTCGAC TGGCCAGTGT TCCTGTAAAC CTGGTGTCGG TGGACTGAAG
    TGCGACCGCT GTGAGCCCGG ATTTTGGAAC TTCCGTGGCA TTGTCACAGA AAACATGAGC
    GGCTGCACAC CGTGCAACTG TGATGGAGTG GGTTCGGTTC GTGATGACTG CGAGCAGATG
    TCAGGCCTGT GCTCCTGTAA GCCTGGAGTT AAGGGCATGA AATGCAACGT GTGCCCTGAT
    GGCAGCAAGA TGGGAGTCAA CGGCTGTGAC AAAGGACCTA AGGCTCCCAA GTCCTGTGCT
    GAGCTTGTGT GCCACTTTGG GTCTTCATGC GTGGAGGTGA ATGGACAGGC TCACTGTGAG
    TGTCCTTCAC CAGATTGTGA TGAGAAAAAC AAGACCAAGG TATGTGGTTC TGATGGGGTC
    ACCTATGCAG ACCGGTGCCA GCTGAAGACC ATCGCATGCC GACAAGATAA AGAGATCAAG
    GTGGAGCATT TAGGCCAGTG TAAAGAATCC ATTGAGCTGA CGGCCAAACC CACCCCCTTC
    CCAACCACCC ACCGCACCAC CCCTCCCACC GCTCACGTCA AGGTCACCAA ACAGCGACAT
    GGCCACCGCA CCACCACTCC GGACAGCTAT GTGGTGGAGG CAATGCCTCC ACCGAGGTTG
    GATGGAGCGC GAAAGTTTTC CAGCCCTTTC ACCACCCACC GTCCCACTAC ACCCTACAAA
    CCACTCCGCA CCTCATCCCC ACCTCTGGAC TTTGACGAGT CGGGCAGTGG AGAGCCCAGT
    GGGGATGATG AGATGGTGAG CGGAATGGAG GAGAGCGGAG AAGAGCCACT TGGTACAACA
    GTAGCTACTA CCACTCTTCC CACAGCTGAG GAGCGATCGT CCTGTGACAA CACAGCGTTC
    GGCTGCTGTC CCGATGGGAA GACGGCCGCC GTCAACTCAG AGGGCACAAA CTGCCCATCC
    ACCATGCGTT TCAGCGGCTT CCTGCACCTG GATAAGGTTG AGGGTCAGGA GGTCTTCTAC
    ACGCCTGAGA TGGAGGACCC CAAATCGGAA CTGTTTGGTG AAACGGCCCG AAGCATCGAG
    AGCGCTATGA ATGAGTTGTT CCGCAAGTCT GATGTCCAGA AAGACTTCCA GAGTGTCCGT
    GTGAGGAATC TATCCCCCAG CAACTCCATA ATAGCACATT TCGAGGCTCA CTTCGACCCA
    GACACAAGGT TCAATGTGGG GGATATTGAG GGAGCCCTTC TCAAACAGCT TAAGGCGTCT
    AAAGACACTG GCATCGTTGT GAAGAAACCA GAGGAAGAGA ACATTCGCAT CAACAACTAT
    GGTCTTTCAA CTGTTCCTTT CTTTACCACC ACAACAACCA CTGTGGCATC TGTCACGACT
    CCCACTACCA CCACCACCAC AATGTCTCCC AGTTCTGCTT ATACCACTCT CCGGCCCATC
    ACTACTAGAC GACCCTTCAC AAGAACCACC ACGCCAGCAC CAGCCACCAC CACTGCACCA
    CATGTGCCCA AGACCACAGT TTCGCTTATG AACAGACTAG GGCCCCACGC TCACAGTCCA
    TGTGACTCTC AGCCCTGCCG ACATGGAGGA ACCTGTGAAG AAGACGACAA TGACTTCACC
    TGTATCTGTC CAGCAGGACG AGGAGGAGCC GTGTGTGAGA AAGTTATAAA GTACTTCATA
    CCATCCTTTG GTGGAAAGTC TTACATGGCG TTCCAGACCA TGAAGGCCTA CCATACTGTA
    CGCATTGCCA TGGAGTTCAG GGCTTCGGAA ATGACTGGGA TATTGCTCTA CAATGGGCAA
    AAAAGCAAAA AGGACTTTCT TTCTCTGACT CTAGTTGAAG GATATGTGGA GCTCAGGTTT
    AACACTGGTT CAGGGACTGC TTCAGTTATC AGTAAAGTCC TGGTAAAGCC GGGTCGCTGG
    CATCAGCTTG TTGTAGTACG TAACAGGCGC AATGCCATGC TGAGCGTGGA CAATGAGCCC
    AGCGTTGAAG GACAAAGTCC TCCAGGAACA GATGGACTCA ACCTGGACAC AGACCTTTTC
    ATCGGCGGAG TGCTTGATGA CATGAGTGCA GACGTAAAGG AGAGGACATC AGTTTCAGCA
    GGTCTGGTGG GCTGCATCCG CATGCTGGAC GTGAACAACC TTGTCTATAA TCTGCAAGAG
    AATGGCGGCA GCGTTCTGTA CGGTTCGGCC ATTGGCGAGT GTGGCAACAA CCCATGCCTG
    CCAAACCCCT GCAAGAACGG AGCGTCCTGT CAGGTCAAAG AAGCCGAGAT TTTCCACTGC
    AAATGTGTCA ACGGTTTCTC TGGCCCGACC TGCGCTGATG CCCACAATCC CTGCGACCCC
    AATAAATGCC ACCCGCCATC ACGGTGCCAG GTCCTTCCAG AGGGAGGTTA CAAATGCGAA
    TGTCCGATGG GACGTGAAGG CAGGCACTGT GAAAAAGTGT CAGACAAGGG AGGCGCTTTC
    ATTCCTTACT TCACTGGAGA TTCTTTTCTG GAGCTCAAAG GCCTCCATAC GTATAACCAG
    GATCTCAGGC AAAAATTCAG CATGACTATC GTTCTACTGG CCAATGACTC CAAAGGCATG
    ATCTTCTACA ATGGTCAGAA GACAGACGGC AAAGGGGATT TCATCTCGCT CTCTCTTAAT
    GACGGCATCC TGGAGTTTAG ATATGACCTG GGCAAAGGGC CAGCTGTCAT TAGGAGTAAA
    GAAAAAATCA AGCTGAACGA GTGGAACACA GTGAATTTGG AACGGGCCAG CAGAAAAGGA
    GAGATTAGCA TTAACGGCAA AGACCCTGTC AGAGGAGAGG CGCCGAAATC ACGCAAGAAT
    CAGCACACGG ATCTCAATCT GAAGGAATCG CTCTTTGTGG GTGGAGCTCC TGATTTCCGC
    AAAGTAGCCA GAGCTGCTGC GATCAAAGAT GGCTTCAAAG GGGCCATTCA GAAGATTACT
    TTGATGGGAA TTCCAATCCT GAAAGCTGAT AATGCCCTTT CGTCCACTGA CGTGTCCATG
    TATCCTAATC ATCCCTGCTC CAAAGACGTT TGTGAGAATG GAGGCCGCTG CAACCCCCAG
    CTGAACAGCT ATGAGTGCGC CTGCCGCCAC GGATTCTCCG GACACCACTG CCAGGACACA
    ATATTTGAGA AGTCCGCTGG GGACACGGAG GCCATTGCGT TTGATGGTCA TACATTCATC
    GAATATCACA ACGGAGTCAC CAAAAGCCAA CTGACCAATG AGATCCCAGA CCCTGAGTCA
    CTGGAGAACC CATCTGACCA GAGCGAGAAA GCCCTGCTGG TGAATAAATT CGAGCTGAGC
    ATCAGGACGG AGGCTACGCA TGGTCTGATT TTGTGGAGCG GAAAGGGAGT TGAGCGCTCT
    GACTACATCG CCCTCGCCAT TGTGGATGGA AGAGTACAGA TGACCTATGA CCTCGGCTCC
    AAGCCTGTGG TGCTGCGCTC CACTGTACGG GTCAACACCA ACCACTGGAT ACGAATTAAG
    GCCAGCAGAG CGCTCAGAGA TGGCTCTCTG CAGGTTGGTA ATGAGGCAGC TGTCACTGGG
    TCTTCACCCT TGGCTGCCAC ACAGCTGGAC ACAGACGGGG CTCTCTGGTT GGGTGGACTG
    GAGGAGCTGT CTGTGGCTCG ACGGCTGCCC AAAGCTTACA GTACAGGCTT CATTGGCTGC
    GTCAAAGACG TGATAGTGGA CGGCGTGGAG CTGCATCTGG TGGAGGACGC CTTAAATACT
    CCCAAAATAT CACACTGTTC TGCCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325507.1
    CDS266..6415
    Translation

    Target ORF information:

    RefSeq Version XM_021469832.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469832.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATAAACC TGTTCACTTC
    ACACCGGCAC CTCCTCCTGA TGGTTGCAGA GGGAAGTTGT GCGGATTTGG AGCGGTGTGT
    GAGAGAGACC AAGCTGACCC ATCTAAAGGA GAGTGTGTGT GTAAGAAGAT TGTGTGTACA
    TCTGTGGTGG CGCCAGTGTG CGGATCTGAC TCCTCCACCT ATTCCAATGA GTGTGAGCTG
    GAGAAGGCTC AGTGCAATAC ACAGAGACGC ATCAAGGTGA TGCGCAAAGG CCCCTGCGCC
    CTCAAGGACC CGTGCAGTGA GGTAACCTGT AGCTTTGGCA GCACCTGCAT CCAGTCATCG
    GATGGTCTGT CCGCCAAGTG TATGTGTCCT CTGAGCTGTG AAAACGTCCC CAAGCATGTG
    GTGTGTGGCA GTGACGGCCT GGACTACCAA AGCGAATGTG AACTCAACAT GAAGGCCTGC
    GCCACTCAGA AGAACATCCG TGTGCATCAC CAGGGCCGCT GCGATCCTTG CAGTGACACT
    CTGAACAGCT TAAGCACAGC CTGTCGAGTC AACCCAAAGA CCTACCAGCA GATTACCTTT
    GCCCCGGCTG AGTCTTGTCC GCCTGACGAA ATGCCCATCT GTGCCAGCGA CGGACACACC
    TACGAGAGCA TGTGTCAGAT GGAGCGCACT GCCCTGAAGA ACAACCTGGA GCTCAAAAAG
    GTCTCTTTAG GCAGCTGCAA GGAAAAAGGG GGCTGCCCGA ATGGTTGTAA GTTCAACGCC
    ATATGTTTGC TGGATAATGG AGAGTTTCGG TGCTCCTGCG ATCCCATACA ATGCGACGGC
    ACGTACAAGC CCCTGTGCGG AAAGGATGGC AAGACATACC CCAACGACTG CGAGAGGAAA
    CTGCAGGAAT GCCGGACCCA AAAAGACATC CCTGTCAAAC AACAAGGGCC CTGTGATCTT
    AACCTGGAGA GCCCCTGCCT GAAGAAGACC TGCGAGTTTG GAGCCGTGTG CGTGGTGAAG
    AACAGCGAAG CAGTGTGTGA ATGCTCAGAC GCCTGTCCGC AGGACCAAGA CCCTGTTTGC
    GGCAGCGATG GGCACACGTA CAGCAGCTCA TGTCAAATGA AGGCCATGGG ATGCGCTCTT
    CAGAAACAGA TACAGATGCA ACACAAAGGA CCATGTGCAG ATGAGTCCTG CACCAACTGC
    TCGTTTGGCG CGATCTGTGA TGCCCAGACT GGCCGGTGCG TGTGCCCTAA AGGCTGTTTG
    GAAACACGGC AGCCCGTATG CGGCAGCGAC GGCACCACGT ATGACAACGA GTGCAAGCTC
    AACGTCCAAG CCTGCACTAA ACAGCTGGAT CTCAAGGTGG TGGCCCATGG AGAATGCAAA
    ACCTGTGGCA GCACTGTGTG TAGCTGGGGG TCGCAGTGCG TGAACAACAA GTGTGAGTGT
    CAGCAGTGCA CTGGTCAGCC GGTGAAACCC GTGTGTGGCA GCGACGGCAA CACATACAAC
    AACGACTGCG AGCTGAGACT GGCCTCCTGT CAGAAACAGA GGAAGATAGA GGTGGCCAAG
    CCTGGCGTCT GCGATGAAGA CTGTGGTTCA GGAGGCTCCG GCTCGGGATC TGAGACTTGT
    GAACAGGACC GATGTCGCCG ATATGGAGGA ACGTGGGACG ATGACATGGA GGACGAGCGA
    TGTGTTTGTG AACTAAACTG TCAGATTGTG CTGCACAGCC CGGTGTGCGG CTCTGATGGA
    AACACGTACT CCAGCGACTG TGAGCTCAAG AAGACCAGGT GCGAAAAGCA AATAGACCTG
    AGTGTCCAAA GCCAAGGGCC CTGTCCAACT ATCGTATCAC CACCTCCAGA GCTTGTTGCC
    TCCCAGCACT GTAGCCAAAC AGTTTATGGA TGTTGTGAAG ATAACAAGAC CGCTGCCCTG
    GGTGTTGGAC TGGCTGGATG TCCTAGTATG TGTCAGTGCA ATCATTACGG CTCATACGGA
    GGCACATGTG ACCCCTCGAC TGGCCAGTGT TCCTGTAAAC CTGGTGTCGG TGGACTGAAG
    TGCGACCGCT GTGAGCCCGG ATTTTGGAAC TTCCGTGGCA TTGTCACAGA AAACATGAGC
    GGCTGCACAC CGTGCAACTG TGATGGAGTG GGTTCGGTTC GTGATGACTG CGAGCAGATG
    TCAGGCCTGT GCTCCTGTAA GCCTGGAGTT AAGGGCATGA AATGCAACGT GTGCCCTGAT
    GGCAGCAAGA TGGGAGTCAA CGGCTGTGAC AAAGGACCTA AGGCTCCCAA GTCCTGTGCT
    GAGCTTGTGT GCCACTTTGG GTCTTCATGC GTGGAGGTGA ATGGACAGGC TCACTGTGAG
    TGTCCTTCAC CAGATTGTGA TGAGAAAAAC AAGACCAAGG TATGTGGTTC TGATGGGGTC
    ACCTATGCAG ACCGGTGCCA GCTGAAGACC ATCGCATGCC GACAAGATAA AGAGATCAAG
    GTGGAGCATT TAGGCCAGTG TAAAGAATCC ATTGAGCTGA CGGCCAAACC CACCCCCTTC
    CCAACCACCC ACCGCACCAC CCCTCCCACC GCTCACGTCA AGGTCACCAA ACAGCGACAT
    GGCCACCGCA CCACCACTCC GGACAGCTAT GTGGTGGAGG CAATGCCTCC ACCGAGGTTG
    GATGGAGCGC GAAAGTTTTC CAGCCCTTTC ACCACCCACC GTCCCACTAC ACCCTACAAA
    CCACTCCGCA CCTCATCCCC ACCTCTGGAC TTTGACGAGT CGGGCAGTGG AGAGCCCAGT
    GGGGATGATG AGATGGTGAG CGGAATGGAG GAGAGCGGAG AAGAGCCACT TGGTACAACA
    GTAGCTACTA CCACTCTTCC CACAGCTGAG GAGCGATCGT CCTGTGACAA CACAGCGTTC
    GGCTGCTGTC CCGATGGGAA GACGGCCGCC GTCAACTCAG AGGGCACAAA CTGCCCATCC
    ACCATGCGTT TCAGCGGCTT CCTGCACCTG GATAAGGTTG AGGGTCAGGA GGTCTTCTAC
    ACGCCTGAGA TGGAGGACCC CAAATCGGAA CTGTTTGGTG AAACGGCCCG AAGCATCGAG
    AGCGCTATGA ATGAGTTGTT CCGCAAGTCT GATGTCCAGA AAGACTTCCA GAGTGTCCGT
    GTGAGGAATC TATCCCCCAG CAACTCCATA ATAGCACATT TCGAGGCTCA CTTCGACCCA
    GACACAAGGT TCAATGTGGG GGATATTGAG GGAGCCCTTC TCAAACAGCT TAAGGCGTCT
    AAAGACACTG GCATCGTTGT GAAGAAACCA GAGGAAGAGA ACATTCGCAT CAACAACTAT
    GGTCTTTCAA CTGTTCCTTT CTTTACCACC ACAACAACCA CTGTGGCATC TGTCACGACT
    CCCACTACCA CCACCACCAC AATGTCTCCC AGTTCTGCTT ATACCACTCT CCGGCCCATC
    ACTACTAGAC GACCCTTCAC AAGAACCACC ACGCCAGCAC CAGCCACCAC CACTGCACCA
    CATGTGCCCA AGACCACAGT TTCGCTTATG AACAGACTAG GGCCCCACGC TCACAGTCCA
    TGTGACTCTC AGCCCTGCCG ACATGGAGGA ACCTGTGAAG AAGACGACAA TGACTTCACC
    TGTATCTGTC CAGCAGGACG AGGAGGAGCC GTGTGTGAGA AAGTTATAAA GTACTTCATA
    CCATCCTTTG GTGGAAAGTC TTACATGGCG TTCCAGACCA TGAAGGCCTA CCATACTGTA
    CGCATTGCCA TGGAGTTCAG GGCTTCGGAA ATGACTGGGA TATTGCTCTA CAATGGGCAA
    AAAAGCAAAA AGGACTTTCT TTCTCTGACT CTAGTTGAAG GATATGTGGA GCTCAGGTTT
    AACACTGGTT CAGGGACTGC TTCAGTTATC AGTAAAGTCC TGGTAAAGCC GGGTCGCTGG
    CATCAGCTTG TTGTAGTACG TAACAGGCGC AATGCCATGC TGAGCGTGGA CAATGAGCCC
    AGCGTTGAAG GACAAAGTCC TCCAGGAACA GATGGACTCA ACCTGGACAC AGACCTTTTC
    ATCGGCGGAG TGCTTGATGA CATGAGTGCA GACGTAAAGG AGAGGACATC AGTTTCAGCA
    GGTCTGGTGG GCTGCATCCG CATGCTGGAC GTGAACAACC TTGTCTATAA TCTGCAAGAG
    AATGGCGGCA GCGTTCTGTA CGGTTCGGCC ATTGGCGAGT GTGGCAACAA CCCATGCCTG
    CCAAACCCCT GCAAGAACGG AGCGTCCTGT CAGGTCAAAG AAGCCGAGAT TTTCCACTGC
    AAATGTGTCA ACGGTTTCTC TGGCCCGACC TGCGCTGATG CCCACAATCC CTGCGACCCC
    AATAAATGCC ACCCGCCATC ACGGTGCCAG GTCCTTCCAG AGGGAGGTTA CAAATGCGAA
    TGTCCGATGG GACGTGAAGG CAGGCACTGT GAAAAAGTGT CAGACAAGGG AGGCGCTTTC
    ATTCCTTACT TCACTGGAGA TTCTTTTCTG GAGCTCAAAG GCCTCCATAC GTATAACCAG
    GATCTCAGGC AAAAATTCAG CATGACTATC GTTCTACTGG CCAATGACTC CAAAGGCATG
    ATCTTCTACA ATGGTCAGAA GACAGACGGC AAAGGGGATT TCATCTCGCT CTCTCTTAAT
    GACGGCATCC TGGAGTTTAG ATATGACCTG GGCAAAGGGC CAGCTGTCAT TAGGAGTAAA
    GAAAAAATCA AGCTGAACGA GTGGAACACA GTGAATTTGG AACGGGCCAG CAGAAAAGGA
    GAGATTAGCA TTAACGGCAA AGACCCTGTC AGAGGAGAGG CGCCGAAATC ACGCAAGAAT
    CAGCACACGG ATCTCAATCT GAAGGAATCG CTCTTTGTGG GTGGAGCTCC TGATTTCCGC
    AAAGTAGCCA GAGCTGCTGC GATCAAAGAT GGCTTCAAAG GGGCCATTCA GAAGATTACT
    TTGATGGGAA TTCCAATCCT GAAAGCTGAT AATGCCCTTT CGTCCACTGA CGTGTCCATG
    TATCCTAATC ATCCCTGCTC CAAAGACGTT TGTGAGAATG GAGGCCGCTG CAACCCCCAG
    CTGAACAGCT ATGAGTGCGC CTGCCGCCAC GGATTCTCCG GACACCACTG CCAGGACACA
    ATATTTGAGA AGTCCGCTGG GGACACGGAG GCCATTGCGT TTGATGGTCA TACATTCATC
    GAATATCACA ACGGAGTCAC CAAAAGCCAA CTGACCAATG AGATCCCAGA CCCTGAGTCA
    CTGGAGAACC CATCTGACCA GAGCGAGAAA GCCCTGCTGG TGAATAAATT CGAGCTGAGC
    ATCAGGACGG AGGCTACGCA TGGTCTGATT TTGTGGAGCG GAAAGGGAGT TGAGCGCTCT
    GACTACATCG CCCTCGCCAT TGTGGATGGA AGAGTACAGA TGACCTATGA CCTCGGCTCC
    AAGCCTGTGG TGCTGCGCTC CACTGTACGG GTCAACACCA ACCACTGGAT ACGAATTAAG
    GCCAGCAGAG CGCTCAGAGA TGGCTCTCTG CAGGTTGGTA ATGAGGCAGC TGTCACTGGG
    TCTTCACCCT TGGCTGCCAC ACAGCTGGAC ACAGACGGGG CTCTCTGGTT GGGTGGACTG
    GAGGAGCTGT CTGTGGCTCG ACGGCTGCCC AAAGCTTACA GTACAGGCTT CATTGGCTGC
    GTCAAAGACG TGATAGTGGA CGGCGTGGAG CTGCATCTGG TGGAGGACGC CTTAAATACT
    CCCAAAATAT CACACTGTTC TGCCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151479
    Clone ID Related Accession (Same CDS sequence) XM_021469833.1
    Accession Version XM_021469833.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product agrin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAAAT CACGCAAGAA TCAGCACACG GATCTCAATC TGAAGGAATC GCTCTTTGTG
    GGTGGAGCTC CTGATTTCCG CAAAGTAGCC AGAGCTGCTG CGATCAAAGA TGGCTTCAAA
    GGGGCCATTC AGAAGATTAC TTTGATGGGA ATTCCAATCC TGAAAGCTGA TAATGCCCTT
    TCGTCCACTG ACGTGTCCAT GTATCCTAAT CATCCCTGCT CCAAAGACGT TTGTGAGAAT
    GGAGGCCGCT GCAACCCCCA GCTGAACAGC TATGAGTGCG CCTGCCGCCA CGGATTCTCC
    GGACACCACT GCCAGGACAC AATATTTGAG AAGTCCGCTG GGGACACGGA GGCCATTGCG
    TTTGATGGTC ATACATTCAT CGAATATCAC AACGGAGTCA CCAAAAGCCA ACTGACCAAT
    GAGATCCCAG ACGAGAAAGC CCTGCTGGTG AATAAATTCG AGCTGAGCAT CAGGACGGAG
    GCTACGCATG GTCTGATTTT GTGGAGCGGA AAGGGAGTTG AGCGCTCTGA CTACATCGCC
    CTCGCCATTG TGGATGGAAG AGTACAGATG ACCTATGACC TCGGCTCCAA GCCTGTGGTG
    CTGCGCTCCA CTGTACGGGT CAACACCAAC CACTGGATAC GAATTAAGGC CAGCAGAGCG
    CTCAGAGATG GCTCTCTGCA GGTTGGTAAT GAGGCAGCTG TCACTGGGTC TTCACCCTTG
    GCTGCCACAC AGCTGGACAC AGACGGGGCT CTCTGGTTGG GTGGACTGGA GGAGCTGTCT
    GTGGCTCGAC GGCTGCCCAA AGCTTACAGT ACAGGCTTCA TTGGCTGCGT CAAAGACGTG
    ATAGTGGACG GCGTGGAGCT GCATCTGGTG GAGGACGCCT TAAATACTCC CAAAATATCA
    CACTGTTCTG CCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325508.1
    CDS266..6403
    Translation

    Target ORF information:

    RefSeq Version XM_021469833.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469833.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAAAT CACGCAAGAA TCAGCACACG GATCTCAATC TGAAGGAATC GCTCTTTGTG
    GGTGGAGCTC CTGATTTCCG CAAAGTAGCC AGAGCTGCTG CGATCAAAGA TGGCTTCAAA
    GGGGCCATTC AGAAGATTAC TTTGATGGGA ATTCCAATCC TGAAAGCTGA TAATGCCCTT
    TCGTCCACTG ACGTGTCCAT GTATCCTAAT CATCCCTGCT CCAAAGACGT TTGTGAGAAT
    GGAGGCCGCT GCAACCCCCA GCTGAACAGC TATGAGTGCG CCTGCCGCCA CGGATTCTCC
    GGACACCACT GCCAGGACAC AATATTTGAG AAGTCCGCTG GGGACACGGA GGCCATTGCG
    TTTGATGGTC ATACATTCAT CGAATATCAC AACGGAGTCA CCAAAAGCCA ACTGACCAAT
    GAGATCCCAG ACGAGAAAGC CCTGCTGGTG AATAAATTCG AGCTGAGCAT CAGGACGGAG
    GCTACGCATG GTCTGATTTT GTGGAGCGGA AAGGGAGTTG AGCGCTCTGA CTACATCGCC
    CTCGCCATTG TGGATGGAAG AGTACAGATG ACCTATGACC TCGGCTCCAA GCCTGTGGTG
    CTGCGCTCCA CTGTACGGGT CAACACCAAC CACTGGATAC GAATTAAGGC CAGCAGAGCG
    CTCAGAGATG GCTCTCTGCA GGTTGGTAAT GAGGCAGCTG TCACTGGGTC TTCACCCTTG
    GCTGCCACAC AGCTGGACAC AGACGGGGCT CTCTGGTTGG GTGGACTGGA GGAGCTGTCT
    GTGGCTCGAC GGCTGCCCAA AGCTTACAGT ACAGGCTTCA TTGGCTGCGT CAAAGACGTG
    ATAGTGGACG GCGTGGAGCT GCATCTGGTG GAGGACGCCT TAAATACTCC CAAAATATCA
    CACTGTTCTG CCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151483
    Clone ID Related Accession (Same CDS sequence) XM_021469837.1
    Accession Version XM_021469837.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product agrin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATAAACC TGTTCACTTC
    ACACCGGCAC CTCCTCCTGA TGGTTGCAGA GGGAAGTTGT GCGGATTTGG AGCGGTGTGT
    GAGAGAGACC AAGCTGACCC ATCTAAAGGA GAGTGTGTGT GTAAGAAGAT TGTGTGTACA
    TCTGTGGTGG CGCCAGTGTG CGGATCTGAC TCCTCCACCT ATTCCAATGA GTGTGAGCTG
    GAGAAGGCTC AGTGCAATAC ACAGAGACGC ATCAAGGTGA TGCGCAAAGG CCCCTGCGCC
    CTCAAGGACC CGTGCAGTGA GGTAACCTGT AGCTTTGGCA GCACCTGCAT CCAGTCATCG
    GATGGTCTGT CCGCCAAGTG TATGTGTCCT CTGAGCTGTG AAAACGTCCC CAAGCATGTG
    GTGTGTGGCA GTGACGGCCT GGACTACCAA AGCGAATGTG AACTCAACAT GAAGGCCTGC
    GCCACTCAGA AGAACATCCG TGTGCATCAC CAGGGCCGCT GCGATCCTTG CAGTGACACT
    CTGAACAGCT TAAGCACAGC CTGTCGAGTC AACCCAAAGA CCTACCAGCA GATTACCTTT
    GCCCCGGCTG AGTCTTGTCC GCCTGACGAA ATGCCCATCT GTGCCAGCGA CGGACACACC
    TACGAGAGCA TGTGTCAGAT GGAGCGCACT GCCCTGAAGA ACAACCTGGA GCTCAAAAAG
    GTCTCTTTAG GCAGCTGCAA GGAAAAAGGG GGCTGCCCGA ATGGTTGTAA GTTCAACGCC
    ATATGTTTGC TGGATAATGG AGAGTTTCGG TGCTCCTGCG ATCCCATACA ATGCGACGGC
    ACGTACAAGC CCCTGTGCGG AAAGGATGGC AAGACATACC CCAACGACTG CGAGAGGAAA
    CTGCAGGAAT GCCGGACCCA AAAAGACATC CCTGTCAAAC AACAAGGGCC CTGTGATCTT
    AACCTGGAGA GCCCCTGCCT GAAGAAGACC TGCGAGTTTG GAGCCGTGTG CGTGGTGAAG
    AACAGCGAAG CAGTGTGTGA ATGCTCAGAC GCCTGTCCGC AGGACCAAGA CCCTGTTTGC
    GGCAGCGATG GGCACACGTA CAGCAGCTCA TGTCAAATGA AGGCCATGGG ATGCGCTCTT
    CAGAAACAGA TACAGATGCA ACACAAAGGA CCATGTGATG AGTCCTGCAC CAACTGCTCG
    TTTGGCGCGA TCTGTGATGC CCAGACTGGC CGGTGCGTGT GCCCTAAAGG CTGTTTGGAA
    ACACGGCAGC CCGTATGCGG CAGCGACGGC ACCACGTATG ACAACGAGTG CAAGCTCAAC
    GTCCAAGCCT GCACTAAACA GCTGGATCTC AAGGTGGTGG CCCATGGAGA ATGCAAAACC
    TGTGGCAGCA CTGTGTGTAG CTGGGGGTCG CAGTGCGTGA ACAACAAGTG TGAGTGTCAG
    CAGTGCACTG GTCAGCCGGT GAAACCCGTG TGTGGCAGCG ACGGCAACAC ATACAACAAC
    GACTGCGAGC TGAGACTGGC CTCCTGTCAG AAACAGAGGA AGATAGAGGT GGCCAAGCCT
    GGCGTCTGCG ATGAAGACTG TGGTTCAGGA GGCTCCGGCT CGGGATCTGA GACTTGTGAA
    CAGGACCGAT GTCGCCGATA TGGAGGAACG TGGGACGATG ACATGGAGGA CGAGCGATGT
    GTTTGTGAAC TAAACTGTCA GATTGTGCTG CACAGCCCGG TGTGCGGCTC TGATGGAAAC
    ACGTACTCCA GCGACTGTGA GCTCAAGAAG ACCAGGTGCG AAAAGCAAAT AGACCTGAGT
    GTCCAAAGCC AAGGGCCCTG TCCAACTATC GTATCACCAC CTCCAGAGCT TGTTGCCTCC
    CAGCACTGTA GCCAAACAGT TTATGGATGT TGTGAAGATA ACAAGACCGC TGCCCTGGGT
    GTTGGACTGG CTGGATGTCC TAGTATGTGT CAGTGCAATC ATTACGGCTC ATACGGAGGC
    ACATGTGACC CCTCGACTGG CCAGTGTTCC TGTAAACCTG GTGTCGGTGG ACTGAAGTGC
    GACCGCTGTG AGCCCGGATT TTGGAACTTC CGTGGCATTG TCACAGAAAA CATGAGCGGC
    TGCACACCGT GCAACTGTGA TGGAGTGGGT TCGGTTCGTG ATGACTGCGA GCAGATGTCA
    GGCCTGTGCT CCTGTAAGCC TGGAGTTAAG GGCATGAAAT GCAACGTGTG CCCTGATGGC
    AGCAAGATGG GAGTCAACGG CTGTGACAAA GGACCTAAGG CTCCCAAGTC CTGTGCTGAG
    CTTGTGTGCC ACTTTGGGTC TTCATGCGTG GAGGTGAATG GACAGGCTCA CTGTGAGTGT
    CCTTCACCAG ATTGTGATGA GAAAAACAAG ACCAAGGTAT GTGGTTCTGA TGGGGTCACC
    TATGCAGACC GGTGCCAGCT GAAGACCATC GCATGCCGAC AAGATAAAGA GATCAAGGTG
    GAGCATTTAG GCCAGTGTAA AGAATCCATT GAGCTGACGG CCAAACCCAC CCCCTTCCCA
    ACCACCCACC GCACCACCCC TCCCACCGCT CACGTCAAGG TCACCAAACA GCGACATGGC
    CACCGCACCA CCACTCCGGA CAGCTATGTG GTGGAGGCAA TGCCTCCACC GAGGTTGGAT
    GGAGCGCGAA AGTTTTCCAG CCCTTTCACC ACCCACCGTC CCACTACACC CTACAAACCA
    CTCCGCACCT CATCCCCACC TCTGGACTTT GACGAGTCGG GCAGTGGAGA GCCCAGTGGG
    GATGATGAGA TGGTGAGCGG AATGGAGGAG AGCGGAGAAG AGCCACTTGG TACAACAGTA
    GCTACTACCA CTCTTCCCAC AGCTGAGGAG CGATCGTCCT GTGACAACAC AGCGTTCGGC
    TGCTGTCCCG ATGGGAAGAC GGCCGCCGTC AACTCAGAGG GCACAAACTG CCCATCCACC
    ATGCGTTTCA GCGGCTTCCT GCACCTGGAT AAGGTTGAGG GTCAGGAGGT CTTCTACACG
    CCTGAGATGG AGGACCCCAA ATCGGAACTG TTTGGTGAAA CGGCCCGAAG CATCGAGAGC
    GCTATGAATG AGTTGTTCCG CAAGTCTGAT GTCCAGAAAG ACTTCCAGAG TGTCCGTGTG
    AGGAATCTAT CCCCCAGCAA CTCCATAATA GCACATTTCG AGGCTCACTT CGACCCAGAC
    ACAAGGTTCA ATGTGGGGGA TATTGAGGGA GCCCTTCTCA AACAGCTTAA GGCGTCTAAA
    GACACTGGCA TCGTTGTGAA GAAACCAGAG GAAGAGAACA TTCGCATCAA CAACTATGGT
    CTTTCAACTG TTCCTTTCTT TACCACCACA ACAACCACTG TGGCATCTGT CACGACTCCC
    ACTACCACCA CCACCACAAT GTCTCCCAGT TCTGCTTATA CCACTCTCCG GCCCATCACT
    ACTAGACGAC CCTTCACAAG AACCACCACG CCAGCACCAG CCACCACCAC TGCACCACAT
    GTGCCCAAGA CCACAGTTTC GCTTATGAAC AGACTAGGGC CCCACGCTCA CAGTCCATGT
    GACTCTCAGC CCTGCCGACA TGGAGGAACC TGTGAAGAAG ACGACAATGA CTTCACCTGT
    ATCTGTCCAG CAGGACGAGG AGGAGCCGTG TGTGAGAAAG TTATAAAGTA CTTCATACCA
    TCCTTTGGTG GAAAGTCTTA CATGGCGTTC CAGACCATGA AGGCCTACCA TACTGTACGC
    ATTGCCATGG AGTTCAGGGC TTCGGAAATG ACTGGGATAT TGCTCTACAA TGGGCAAAAA
    AGCAAAAAGG ACTTTCTTTC TCTGACTCTA GTTGAAGGAT ATGTGGAGCT CAGGTTTAAC
    ACTGGTTCAG GGACTGCTTC AGTTATCAGT AAAGTCCTGG TAAAGCCGGG TCGCTGGCAT
    CAGCTTGTTG TAGTACGTAA CAGGCGCAAT GCCATGCTGA GCGTGGACAA TGAGCCCAGC
    GTTGAAGGAC AAAGTCCTCC AGGAACAGAT GGACTCAACC TGGACACAGA CCTTTTCATC
    GGCGGAGTGC TTGATGACAT GAGTGCAGAC GTAAAGGAGA GGACATCAGT TTCAGCAGGT
    CTGGTGGGCT GCATCCGCAT GCTGGACGTG AACAACCTTG TCTATAATCT GCAAGAGAAT
    GGCGGCAGCG TTCTGTACGG TTCGGCCATT GGCGAGTGTG GCAACAACCC ATGCCTGCCA
    AACCCCTGCA AGAACGGAGC GTCCTGTCAG GTCAAAGAAG CCGAGATTTT CCACTGCAAA
    TGTGTCAACG GTTTCTCTGG CCCGACCTGC GCTGATGCCC ACAATCCCTG CGACCCCAAT
    AAATGCCACC CGCCATCACG GTGCCAGGTC CTTCCAGAGG GAGGTTACAA ATGCGAATGT
    CCGATGGGAC GTGAAGGCAG GCACTGTGAA AAAGTGTCAG ACAAGGGAGG CGCTTTCATT
    CCTTACTTCA CTGGAGATTC TTTTCTGGAG CTCAAAGGCC TCCATACGTA TAACCAGGAT
    CTCAGGCAAA AATTCAGCAT GACTATCGTT CTACTGGCCA ATGACTCCAA AGGCATGATC
    TTCTACAATG GTCAGAAGAC AGACGGCAAA GGGGATTTCA TCTCGCTCTC TCTTAATGAC
    GGCATCCTGG AGTTTAGATA TGACCTGGGC AAAGGGCCAG CTGTCATTAG GAGTAAAGAA
    AAAATCAAGC TGAACGAGTG GAACACAGTG AATTTGGAAC GGGCCAGCAG AAAAGGAGAG
    ATTAGCATTA ACGGCAAAGA CCCTGTCAGA GGAGAGGCGC CGAAATCACG CAAGAATCAG
    CACACGGATC TCAATCTGAA GGAATCGCTC TTTGTGGGTG GAGCTCCTGA TTTCCGCAAA
    GTAGCCAGAG CTGCTGCGAT CAAAGATGGC TTCAAAGGGG CCATTCAGAA GATTACTTTG
    ATGGGAATTC CAATCCTGAA AGCTGATAAT GCCCTTTCGT CCACTGACGT GTCCATGTAT
    CCTAATCATC CCTGCTCCAA AGACGTTTGT GAGAATGGAG GCCGCTGCAA CCCCCAGCTG
    AACAGCTATG AGTGCGCCTG CCGCCACGGA TTCTCCGGAC ACCACTGCCA GGACACAATA
    TTTGAGAAGT CCGCTGGGGA CACGGAGGCC ATTGCGTTTG ATGGTCATAC ATTCATCGAA
    TATCACAACG GAGTCACCAA AAGCGAGAAA GCCCTGCTGG TGAATAAATT CGAGCTGAGC
    ATCAGGACGG AGGCTACGCA TGGTCTGATT TTGTGGAGCG GAAAGGGAGT TGAGCGCTCT
    GACTACATCG CCCTCGCCAT TGTGGATGGA AGAGTACAGA TGACCTATGA CCTCGGCTCC
    AAGCCTGTGG TGCTGCGCTC CACTGTACGG GTCAACACCA ACCACTGGAT ACGAATTAAG
    GCCAGCAGAG CGCTCAGAGA TGGCTCTCTG CAGGTTGGTA ATGAGGCAGC TGTCACTGGG
    TCTTCACCCT TGGCTGCCAC ACAGCTGGAC ACAGACGGGG CTCTCTGGTT GGGTGGACTG
    GAGGAGCTGT CTGTGGCTCG ACGGCTGCCC AAAGCTTACA GTACAGGCTT CATTGGCTGC
    GTCAAAGACG TGATAGTGGA CGGCGTGGAG CTGCATCTGG TGGAGGACGC CTTAAATACT
    CCCAAAATAT CACACTGTTC TGCCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325512.1
    CDS265..6354
    Translation

    Target ORF information:

    RefSeq Version XM_021469837.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469837.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATAAACC TGTTCACTTC
    ACACCGGCAC CTCCTCCTGA TGGTTGCAGA GGGAAGTTGT GCGGATTTGG AGCGGTGTGT
    GAGAGAGACC AAGCTGACCC ATCTAAAGGA GAGTGTGTGT GTAAGAAGAT TGTGTGTACA
    TCTGTGGTGG CGCCAGTGTG CGGATCTGAC TCCTCCACCT ATTCCAATGA GTGTGAGCTG
    GAGAAGGCTC AGTGCAATAC ACAGAGACGC ATCAAGGTGA TGCGCAAAGG CCCCTGCGCC
    CTCAAGGACC CGTGCAGTGA GGTAACCTGT AGCTTTGGCA GCACCTGCAT CCAGTCATCG
    GATGGTCTGT CCGCCAAGTG TATGTGTCCT CTGAGCTGTG AAAACGTCCC CAAGCATGTG
    GTGTGTGGCA GTGACGGCCT GGACTACCAA AGCGAATGTG AACTCAACAT GAAGGCCTGC
    GCCACTCAGA AGAACATCCG TGTGCATCAC CAGGGCCGCT GCGATCCTTG CAGTGACACT
    CTGAACAGCT TAAGCACAGC CTGTCGAGTC AACCCAAAGA CCTACCAGCA GATTACCTTT
    GCCCCGGCTG AGTCTTGTCC GCCTGACGAA ATGCCCATCT GTGCCAGCGA CGGACACACC
    TACGAGAGCA TGTGTCAGAT GGAGCGCACT GCCCTGAAGA ACAACCTGGA GCTCAAAAAG
    GTCTCTTTAG GCAGCTGCAA GGAAAAAGGG GGCTGCCCGA ATGGTTGTAA GTTCAACGCC
    ATATGTTTGC TGGATAATGG AGAGTTTCGG TGCTCCTGCG ATCCCATACA ATGCGACGGC
    ACGTACAAGC CCCTGTGCGG AAAGGATGGC AAGACATACC CCAACGACTG CGAGAGGAAA
    CTGCAGGAAT GCCGGACCCA AAAAGACATC CCTGTCAAAC AACAAGGGCC CTGTGATCTT
    AACCTGGAGA GCCCCTGCCT GAAGAAGACC TGCGAGTTTG GAGCCGTGTG CGTGGTGAAG
    AACAGCGAAG CAGTGTGTGA ATGCTCAGAC GCCTGTCCGC AGGACCAAGA CCCTGTTTGC
    GGCAGCGATG GGCACACGTA CAGCAGCTCA TGTCAAATGA AGGCCATGGG ATGCGCTCTT
    CAGAAACAGA TACAGATGCA ACACAAAGGA CCATGTGATG AGTCCTGCAC CAACTGCTCG
    TTTGGCGCGA TCTGTGATGC CCAGACTGGC CGGTGCGTGT GCCCTAAAGG CTGTTTGGAA
    ACACGGCAGC CCGTATGCGG CAGCGACGGC ACCACGTATG ACAACGAGTG CAAGCTCAAC
    GTCCAAGCCT GCACTAAACA GCTGGATCTC AAGGTGGTGG CCCATGGAGA ATGCAAAACC
    TGTGGCAGCA CTGTGTGTAG CTGGGGGTCG CAGTGCGTGA ACAACAAGTG TGAGTGTCAG
    CAGTGCACTG GTCAGCCGGT GAAACCCGTG TGTGGCAGCG ACGGCAACAC ATACAACAAC
    GACTGCGAGC TGAGACTGGC CTCCTGTCAG AAACAGAGGA AGATAGAGGT GGCCAAGCCT
    GGCGTCTGCG ATGAAGACTG TGGTTCAGGA GGCTCCGGCT CGGGATCTGA GACTTGTGAA
    CAGGACCGAT GTCGCCGATA TGGAGGAACG TGGGACGATG ACATGGAGGA CGAGCGATGT
    GTTTGTGAAC TAAACTGTCA GATTGTGCTG CACAGCCCGG TGTGCGGCTC TGATGGAAAC
    ACGTACTCCA GCGACTGTGA GCTCAAGAAG ACCAGGTGCG AAAAGCAAAT AGACCTGAGT
    GTCCAAAGCC AAGGGCCCTG TCCAACTATC GTATCACCAC CTCCAGAGCT TGTTGCCTCC
    CAGCACTGTA GCCAAACAGT TTATGGATGT TGTGAAGATA ACAAGACCGC TGCCCTGGGT
    GTTGGACTGG CTGGATGTCC TAGTATGTGT CAGTGCAATC ATTACGGCTC ATACGGAGGC
    ACATGTGACC CCTCGACTGG CCAGTGTTCC TGTAAACCTG GTGTCGGTGG ACTGAAGTGC
    GACCGCTGTG AGCCCGGATT TTGGAACTTC CGTGGCATTG TCACAGAAAA CATGAGCGGC
    TGCACACCGT GCAACTGTGA TGGAGTGGGT TCGGTTCGTG ATGACTGCGA GCAGATGTCA
    GGCCTGTGCT CCTGTAAGCC TGGAGTTAAG GGCATGAAAT GCAACGTGTG CCCTGATGGC
    AGCAAGATGG GAGTCAACGG CTGTGACAAA GGACCTAAGG CTCCCAAGTC CTGTGCTGAG
    CTTGTGTGCC ACTTTGGGTC TTCATGCGTG GAGGTGAATG GACAGGCTCA CTGTGAGTGT
    CCTTCACCAG ATTGTGATGA GAAAAACAAG ACCAAGGTAT GTGGTTCTGA TGGGGTCACC
    TATGCAGACC GGTGCCAGCT GAAGACCATC GCATGCCGAC AAGATAAAGA GATCAAGGTG
    GAGCATTTAG GCCAGTGTAA AGAATCCATT GAGCTGACGG CCAAACCCAC CCCCTTCCCA
    ACCACCCACC GCACCACCCC TCCCACCGCT CACGTCAAGG TCACCAAACA GCGACATGGC
    CACCGCACCA CCACTCCGGA CAGCTATGTG GTGGAGGCAA TGCCTCCACC GAGGTTGGAT
    GGAGCGCGAA AGTTTTCCAG CCCTTTCACC ACCCACCGTC CCACTACACC CTACAAACCA
    CTCCGCACCT CATCCCCACC TCTGGACTTT GACGAGTCGG GCAGTGGAGA GCCCAGTGGG
    GATGATGAGA TGGTGAGCGG AATGGAGGAG AGCGGAGAAG AGCCACTTGG TACAACAGTA
    GCTACTACCA CTCTTCCCAC AGCTGAGGAG CGATCGTCCT GTGACAACAC AGCGTTCGGC
    TGCTGTCCCG ATGGGAAGAC GGCCGCCGTC AACTCAGAGG GCACAAACTG CCCATCCACC
    ATGCGTTTCA GCGGCTTCCT GCACCTGGAT AAGGTTGAGG GTCAGGAGGT CTTCTACACG
    CCTGAGATGG AGGACCCCAA ATCGGAACTG TTTGGTGAAA CGGCCCGAAG CATCGAGAGC
    GCTATGAATG AGTTGTTCCG CAAGTCTGAT GTCCAGAAAG ACTTCCAGAG TGTCCGTGTG
    AGGAATCTAT CCCCCAGCAA CTCCATAATA GCACATTTCG AGGCTCACTT CGACCCAGAC
    ACAAGGTTCA ATGTGGGGGA TATTGAGGGA GCCCTTCTCA AACAGCTTAA GGCGTCTAAA
    GACACTGGCA TCGTTGTGAA GAAACCAGAG GAAGAGAACA TTCGCATCAA CAACTATGGT
    CTTTCAACTG TTCCTTTCTT TACCACCACA ACAACCACTG TGGCATCTGT CACGACTCCC
    ACTACCACCA CCACCACAAT GTCTCCCAGT TCTGCTTATA CCACTCTCCG GCCCATCACT
    ACTAGACGAC CCTTCACAAG AACCACCACG CCAGCACCAG CCACCACCAC TGCACCACAT
    GTGCCCAAGA CCACAGTTTC GCTTATGAAC AGACTAGGGC CCCACGCTCA CAGTCCATGT
    GACTCTCAGC CCTGCCGACA TGGAGGAACC TGTGAAGAAG ACGACAATGA CTTCACCTGT
    ATCTGTCCAG CAGGACGAGG AGGAGCCGTG TGTGAGAAAG TTATAAAGTA CTTCATACCA
    TCCTTTGGTG GAAAGTCTTA CATGGCGTTC CAGACCATGA AGGCCTACCA TACTGTACGC
    ATTGCCATGG AGTTCAGGGC TTCGGAAATG ACTGGGATAT TGCTCTACAA TGGGCAAAAA
    AGCAAAAAGG ACTTTCTTTC TCTGACTCTA GTTGAAGGAT ATGTGGAGCT CAGGTTTAAC
    ACTGGTTCAG GGACTGCTTC AGTTATCAGT AAAGTCCTGG TAAAGCCGGG TCGCTGGCAT
    CAGCTTGTTG TAGTACGTAA CAGGCGCAAT GCCATGCTGA GCGTGGACAA TGAGCCCAGC
    GTTGAAGGAC AAAGTCCTCC AGGAACAGAT GGACTCAACC TGGACACAGA CCTTTTCATC
    GGCGGAGTGC TTGATGACAT GAGTGCAGAC GTAAAGGAGA GGACATCAGT TTCAGCAGGT
    CTGGTGGGCT GCATCCGCAT GCTGGACGTG AACAACCTTG TCTATAATCT GCAAGAGAAT
    GGCGGCAGCG TTCTGTACGG TTCGGCCATT GGCGAGTGTG GCAACAACCC ATGCCTGCCA
    AACCCCTGCA AGAACGGAGC GTCCTGTCAG GTCAAAGAAG CCGAGATTTT CCACTGCAAA
    TGTGTCAACG GTTTCTCTGG CCCGACCTGC GCTGATGCCC ACAATCCCTG CGACCCCAAT
    AAATGCCACC CGCCATCACG GTGCCAGGTC CTTCCAGAGG GAGGTTACAA ATGCGAATGT
    CCGATGGGAC GTGAAGGCAG GCACTGTGAA AAAGTGTCAG ACAAGGGAGG CGCTTTCATT
    CCTTACTTCA CTGGAGATTC TTTTCTGGAG CTCAAAGGCC TCCATACGTA TAACCAGGAT
    CTCAGGCAAA AATTCAGCAT GACTATCGTT CTACTGGCCA ATGACTCCAA AGGCATGATC
    TTCTACAATG GTCAGAAGAC AGACGGCAAA GGGGATTTCA TCTCGCTCTC TCTTAATGAC
    GGCATCCTGG AGTTTAGATA TGACCTGGGC AAAGGGCCAG CTGTCATTAG GAGTAAAGAA
    AAAATCAAGC TGAACGAGTG GAACACAGTG AATTTGGAAC GGGCCAGCAG AAAAGGAGAG
    ATTAGCATTA ACGGCAAAGA CCCTGTCAGA GGAGAGGCGC CGAAATCACG CAAGAATCAG
    CACACGGATC TCAATCTGAA GGAATCGCTC TTTGTGGGTG GAGCTCCTGA TTTCCGCAAA
    GTAGCCAGAG CTGCTGCGAT CAAAGATGGC TTCAAAGGGG CCATTCAGAA GATTACTTTG
    ATGGGAATTC CAATCCTGAA AGCTGATAAT GCCCTTTCGT CCACTGACGT GTCCATGTAT
    CCTAATCATC CCTGCTCCAA AGACGTTTGT GAGAATGGAG GCCGCTGCAA CCCCCAGCTG
    AACAGCTATG AGTGCGCCTG CCGCCACGGA TTCTCCGGAC ACCACTGCCA GGACACAATA
    TTTGAGAAGT CCGCTGGGGA CACGGAGGCC ATTGCGTTTG ATGGTCATAC ATTCATCGAA
    TATCACAACG GAGTCACCAA AAGCGAGAAA GCCCTGCTGG TGAATAAATT CGAGCTGAGC
    ATCAGGACGG AGGCTACGCA TGGTCTGATT TTGTGGAGCG GAAAGGGAGT TGAGCGCTCT
    GACTACATCG CCCTCGCCAT TGTGGATGGA AGAGTACAGA TGACCTATGA CCTCGGCTCC
    AAGCCTGTGG TGCTGCGCTC CACTGTACGG GTCAACACCA ACCACTGGAT ACGAATTAAG
    GCCAGCAGAG CGCTCAGAGA TGGCTCTCTG CAGGTTGGTA ATGAGGCAGC TGTCACTGGG
    TCTTCACCCT TGGCTGCCAC ACAGCTGGAC ACAGACGGGG CTCTCTGGTT GGGTGGACTG
    GAGGAGCTGT CTGTGGCTCG ACGGCTGCCC AAAGCTTACA GTACAGGCTT CATTGGCTGC
    GTCAAAGACG TGATAGTGGA CGGCGTGGAG CTGCATCTGG TGGAGGACGC CTTAAATACT
    CCCAAAATAT CACACTGTTC TGCCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57387
    Clone ID Related Accession (Same CDS sequence) XM_017353587.1
    Accession Version XM_017353587.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6144bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product agrin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGAT
    GAGTCCTGCA CCAACTGCTC GTTTGGCGCG ATCTGTGATG CCCAGACTGG CCGGTGCGTG
    TGCCCTAAAG GCTGTTTGGA AACACGGCAG CCCGTATGCG GCAGCGACGG CACCACGTAT
    GACAACGAGT GCAAGCTCAA CGTCCAAGCC TGCACTAAAC AGCTGGATCT CAAGGTGGTG
    GCCCATGGAG AATGCAAAAC CTGTGGCAGC ACTGTGTGTA GCTGGGGGTC GCAGTGCGTG
    AACAACAAGT GTGAGTGTCA GCAGTGCACT GGTCAGCCGG TGAAACCCGT GTGTGGCAGC
    GACGGCAACA CATACAACAA CGACTGCGAG CTGAGACTGG CCTCCTGTCA GAAACAGAGG
    AAGATAGAGG TGGCCAAGCC TGGCGTCTGC GATGAAGACT GTGGTTCAGG AGGCTCCGGC
    TCGGGATCTG AGACTTGTGA ACAGGACCGA TGTCGCCGAT ATGGAGGAAC GTGGGACGAT
    GACATGGAGG ACGAGCGATG TGTTTGTGAA CTAAACTGTC AGATTGTGCT GCACAGCCCG
    GTGTGCGGCT CTGATGGAAA CACGTACTCC AGCGACTGTG AGCTCAAGAA GACCAGGTGC
    GAAAAGCAAA TAGACCTGAG TGTCCAAAGC CAAGGGCCCT GTCCAACTAT CGTATCACCA
    CCTCCAGAGC TTGTTGCCTC CCAGCACTGT AGCCAAACAG TTTATGGATG TTGTGAAGAT
    AACAAGACCG CTGCCCTGGG TGTTGGACTG GCTGGATGTC CTAGTATGTG TCAGTGCAAT
    CATTACGGCT CATACGGAGG CACATGTGAC CCCTCGACTG GCCAGTGTTC CTGTAAACCT
    GGTGTCGGTG GACTGAAGTG CGACCGCTGT GAGCCCGGAT TTTGGAACTT CCGTGGCATT
    GTCACAGAAA ACATGAGCGG CTGCACACCG TGCAACTGTG ATGGAGTGGG TTCGGTTCGT
    GATGACTGCG AGCAGATGTC AGGCCTGTGC TCCTGTAAGC CTGGAGTTAA GGGCATGAAA
    TGCAACGTGT GCCCTGATGG CAGCAAGATG GGAGTCAACG GCTGTGACAA AGGACCTAAG
    GCTCCCAAGT CCTGTGCTGA GCTTGTGTGC CACTTTGGGT CTTCATGCGT GGAGGTGAAT
    GGACAGGCTC ACTGTGAGTG TCCTTCACCA GATTGTGATG AGAAAAACAA GACCAAGGTA
    TGTGGTTCTG ATGGGGTCAC CTATGCAGAC CGGTGCCAGC TGAAGACCAT CGCATGCCGA
    CAAGATAAAG AGATCAAGGT GGAGCATTTA GGCCAGTGTA AAGAATCCAT TGAGCTGACG
    GCCAAACCCA CCCCCTTCCC AACCACCCAC CGCACCACCC CTCCCACCGC TCACGTCAAG
    GTCACCAAAC AGCGACATGG CCACCGCACC ACCACTCCGG ACAGCTATGT GGTGGAGGCA
    ATGCCTCCAC CGAGGTTGGA TGGAGCGCGA AAGTTTTCCA GCCCTTTCAC CACCCACCGT
    CCCACTACAC CCTACAAACC ACTCCGCACC TCATCCCCAC CTCTGGACTT TGACGAGTCG
    GGCAGTGGAG AGCCCAGTGG GGATGATGAG ATGGTGAGCG GAATGGAGGA GAGCGGAGAA
    GAGCCACTTG GTACAACAGT AGCTACTACC ACTCTTCCCA CAGCTGAGGA GCGATCGTCC
    TGTGACAACA CAGCGTTCGG CTGCTGTCCC GATGGGAAGA CGGCCGCCGT CAACTCAGAG
    GGCACAAACT GCCCATCCAC CATGCGTTTC AGCGGCTTCC TGCACCTGGA TAAGGTTGAG
    GGTCAGGAGG TCTTCTACAC GCCTGAGATG GAGGACCCCA AATCGGAACT GTTTGGTGAA
    ACGGCCCGAA GCATCGAGAG CGCTATGAAT GAGTTGTTCC GCAAGTCTGA TGTCCAGAAA
    GACTTCCAGA GTGTCCGTGT GAGGAATCTA TCCCCCAGCA ACTCCATAAT AGCACATTTC
    GAGGCTCACT TCGACCCAGA CACAAGGTTC AATGTGGGGG ATATTGAGGG AGCCCTTCTC
    AAACAGCTTA AGGCGTCTAA AGACACTGGC ATCGTTGTGA AGAAACCAGA GGAAGAGAAC
    ATTCGCATCA ACAACTATGG TCTTTCAACT GTTCCTTTCT TTACCACCAC AACAACCACT
    GTGGCATCTG TCACGACTCC CACTACCACC ACCACCACAA TGTCTCCCAG TTCTGCTTAT
    ACCACTCTCC GGCCCATCAC TACTAGACGA CCCTTCACAA GAACCACCAC GCCAGCACCA
    GCCACCACCA CTGCACCACA TGTGCCCAAG ACCACAGTTT CGCTTATGAA CAGACTAGGG
    CCCCACGCTC ACAGTCCATG TGACTCTCAG CCCTGCCGAC ATGGAGGAAC CTGTGAAGAA
    GACGACAATG ACTTCACCTG TATCTGTCCA GCAGGACGAG GAGGAGCCGT GTGTGAGAAA
    GTTATAAAGT ACTTCATACC ATCCTTTGGT GGAAAGTCTT ACATGGCGTT CCAGACCATG
    AAGGCCTACC ATACTGTACG CATTGCCATG GAGTTCAGGG CTTCGGAAAT GACTGGGATA
    TTGCTCTACA ATGGGCAAAA AAGCAAAAAG GACTTTCTTT CTCTGACTCT AGTTGAAGGA
    TATGTGGAGC TCAGGTTTAA CACTGGTTCA GGGACTGCTT CAGTTATCAG TAAAGTCCTG
    GTAAAGCCGG GTCGCTGGCA TCAGCTTGTT GTAGTACGTA ACAGGCGCAA TGCCATGCTG
    AGCGTGGACA ATGAGCCCAG CGTTGAAGGA CAAAGTCCTC CAGGAACAGA TGGACTCAAC
    CTGGACACAG ACCTTTTCAT CGGCGGAGTG CTTGATGACA TGAGTGCAGA CGTAAAGGAG
    AGGACATCAG TTTCAGCAGG TCTGGTGGGC TGCATCCGCA TGCTGGACGT GAACAACCTT
    GTCTATAATC TGCAAGAGAA TGGCGGCAGC GTTCTGTACG GTTCGGCCAT TGGCGAGTGT
    GGCAACAACC CATGCCTGCC AAACCCCTGC AAGAACGGAG CGTCCTGTCA GGTCAAAGAA
    GCCGAGATTT TCCACTGCAA ATGTGTCAAC GGTTTCTCTG GCCCGACCTG CGCTGATGCC
    CACAATCCCT GCGACCCCAA TAAATGCCAC CCGCCATCAC GGTGCCAGGT CCTTCCAGAG
    GGAGGTTACA AATGCGAATG TCCGATGGGA CGTGAAGGCA GGCACTGTGA AAAAGTGTCA
    GACAAGGGAG GCGCTTTCAT TCCTTACTTC ACTGGAGATT CTTTTCTGGA GCTCAAAGGC
    CTCCATACGT ATAACCAGGA TCTCAGGCAA AAATTCAGCA TGACTATCGT TCTACTGGCC
    AATGACTCCA AAGGCATGAT CTTCTACAAT GGTCAGAAGA CAGACGGCAA AGGGGATTTC
    ATCTCGCTCT CTCTTAATGA CGGCATCCTG GAGTTTAGAT ATGACCTGGG CAAAGGGCCA
    GCTGTCATTA GGAGTAAAGA AAAAATCAAG CTGAACGAGT GGAACACAGT GAATTTGGAA
    CGGGCCAGCA GAAAAGGAGA GATTAGCATT AACGGCAAAG ACCCTGTCAG AGGAGAGGCG
    CCGAAATCAC GCAAGAATCA GCACACGGAT CTCAATCTGA AGGAATCGCT CTTTGTGGGT
    GGAGCTCCTG ATTTCCGCAA AGTAGCCAGA GCTGCTGCGA TCAAAGATGG CTTCAAAGGG
    GCCATTCAGA AGATTACTTT GATGGGAATT CCAATCCTGA AAGCTGATAA TGCCCTTTCG
    TCCACTGACG TGTCCATGTA TCCTAATCAT CCCTGCTCCA AAGACGTTTG TGAGAATGGA
    GGCCGCTGCA ACCCCCAGCT GAACAGCTAT GAGTGCGCCT GCCGCCACGG ATTCTCCGGA
    CACCACTGCC AGGACACAAT ATTTGAGAAG TCCGCTGGGG ACACGGAGGC CATTGCGTTT
    GATGGTCATA CATTCATCGA ATATCACAAC GGAGTCACCA AAAGCCCTGA GTCACTGGAG
    AACCCATCTG ACCAGAGCGA GAAAGCCCTG CTGGTGAATA AATTCGAGCT GAGCATCAGG
    ACGGAGGCTA CGCATGGTCT GATTTTGTGG AGCGGAAAGG GAGTTGAGCG CTCTGACTAC
    ATCGCCCTCG CCATTGTGGA TGGAAGAGTA CAGATGACCT ATGACCTCGG CTCCAAGCCT
    GTGGTGCTGC GCTCCACTGT ACGGGTCAAC ACCAACCACT GGATACGAAT TAAGGCCAGC
    AGAGCGCTCA GAGATGGCTC TCTGCAGGTT GGTAATGAGG CAGCTGTCAC TGGGTCTTCA
    CCCTTGGCTG CCACACAGCT GGACACAGAC GGGGCTCTCT GGTTGGGTGG ACTGGAGGAG
    CTGTCTGTGG CTCGACGGCT GCCCAAAGCT TACAGTACAG GCTTCATTGG CTGCGTCAAA
    GACGTGATAG TGGACGGCGT GGAGCTGCAT CTGGTGGAGG ACGCCTTAAA TACTCCCAAA
    ATATCACACT GTTCTGCCAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209076.1
    CDS276..6419
    Translation

    Target ORF information:

    RefSeq Version XM_017353587.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353587.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGAT
    GAGTCCTGCA CCAACTGCTC GTTTGGCGCG ATCTGTGATG CCCAGACTGG CCGGTGCGTG
    TGCCCTAAAG GCTGTTTGGA AACACGGCAG CCCGTATGCG GCAGCGACGG CACCACGTAT
    GACAACGAGT GCAAGCTCAA CGTCCAAGCC TGCACTAAAC AGCTGGATCT CAAGGTGGTG
    GCCCATGGAG AATGCAAAAC CTGTGGCAGC ACTGTGTGTA GCTGGGGGTC GCAGTGCGTG
    AACAACAAGT GTGAGTGTCA GCAGTGCACT GGTCAGCCGG TGAAACCCGT GTGTGGCAGC
    GACGGCAACA CATACAACAA CGACTGCGAG CTGAGACTGG CCTCCTGTCA GAAACAGAGG
    AAGATAGAGG TGGCCAAGCC TGGCGTCTGC GATGAAGACT GTGGTTCAGG AGGCTCCGGC
    TCGGGATCTG AGACTTGTGA ACAGGACCGA TGTCGCCGAT ATGGAGGAAC GTGGGACGAT
    GACATGGAGG ACGAGCGATG TGTTTGTGAA CTAAACTGTC AGATTGTGCT GCACAGCCCG
    GTGTGCGGCT CTGATGGAAA CACGTACTCC AGCGACTGTG AGCTCAAGAA GACCAGGTGC
    GAAAAGCAAA TAGACCTGAG TGTCCAAAGC CAAGGGCCCT GTCCAACTAT CGTATCACCA
    CCTCCAGAGC TTGTTGCCTC CCAGCACTGT AGCCAAACAG TTTATGGATG TTGTGAAGAT
    AACAAGACCG CTGCCCTGGG TGTTGGACTG GCTGGATGTC CTAGTATGTG TCAGTGCAAT
    CATTACGGCT CATACGGAGG CACATGTGAC CCCTCGACTG GCCAGTGTTC CTGTAAACCT
    GGTGTCGGTG GACTGAAGTG CGACCGCTGT GAGCCCGGAT TTTGGAACTT CCGTGGCATT
    GTCACAGAAA ACATGAGCGG CTGCACACCG TGCAACTGTG ATGGAGTGGG TTCGGTTCGT
    GATGACTGCG AGCAGATGTC AGGCCTGTGC TCCTGTAAGC CTGGAGTTAA GGGCATGAAA
    TGCAACGTGT GCCCTGATGG CAGCAAGATG GGAGTCAACG GCTGTGACAA AGGACCTAAG
    GCTCCCAAGT CCTGTGCTGA GCTTGTGTGC CACTTTGGGT CTTCATGCGT GGAGGTGAAT
    GGACAGGCTC ACTGTGAGTG TCCTTCACCA GATTGTGATG AGAAAAACAA GACCAAGGTA
    TGTGGTTCTG ATGGGGTCAC CTATGCAGAC CGGTGCCAGC TGAAGACCAT CGCATGCCGA
    CAAGATAAAG AGATCAAGGT GGAGCATTTA GGCCAGTGTA AAGAATCCAT TGAGCTGACG
    GCCAAACCCA CCCCCTTCCC AACCACCCAC CGCACCACCC CTCCCACCGC TCACGTCAAG
    GTCACCAAAC AGCGACATGG CCACCGCACC ACCACTCCGG ACAGCTATGT GGTGGAGGCA
    ATGCCTCCAC CGAGGTTGGA TGGAGCGCGA AAGTTTTCCA GCCCTTTCAC CACCCACCGT
    CCCACTACAC CCTACAAACC ACTCCGCACC TCATCCCCAC CTCTGGACTT TGACGAGTCG
    GGCAGTGGAG AGCCCAGTGG GGATGATGAG ATGGTGAGCG GAATGGAGGA GAGCGGAGAA
    GAGCCACTTG GTACAACAGT AGCTACTACC ACTCTTCCCA CAGCTGAGGA GCGATCGTCC
    TGTGACAACA CAGCGTTCGG CTGCTGTCCC GATGGGAAGA CGGCCGCCGT CAACTCAGAG
    GGCACAAACT GCCCATCCAC CATGCGTTTC AGCGGCTTCC TGCACCTGGA TAAGGTTGAG
    GGTCAGGAGG TCTTCTACAC GCCTGAGATG GAGGACCCCA AATCGGAACT GTTTGGTGAA
    ACGGCCCGAA GCATCGAGAG CGCTATGAAT GAGTTGTTCC GCAAGTCTGA TGTCCAGAAA
    GACTTCCAGA GTGTCCGTGT GAGGAATCTA TCCCCCAGCA ACTCCATAAT AGCACATTTC
    GAGGCTCACT TCGACCCAGA CACAAGGTTC AATGTGGGGG ATATTGAGGG AGCCCTTCTC
    AAACAGCTTA AGGCGTCTAA AGACACTGGC ATCGTTGTGA AGAAACCAGA GGAAGAGAAC
    ATTCGCATCA ACAACTATGG TCTTTCAACT GTTCCTTTCT TTACCACCAC AACAACCACT
    GTGGCATCTG TCACGACTCC CACTACCACC ACCACCACAA TGTCTCCCAG TTCTGCTTAT
    ACCACTCTCC GGCCCATCAC TACTAGACGA CCCTTCACAA GAACCACCAC GCCAGCACCA
    GCCACCACCA CTGCACCACA TGTGCCCAAG ACCACAGTTT CGCTTATGAA CAGACTAGGG
    CCCCACGCTC ACAGTCCATG TGACTCTCAG CCCTGCCGAC ATGGAGGAAC CTGTGAAGAA
    GACGACAATG ACTTCACCTG TATCTGTCCA GCAGGACGAG GAGGAGCCGT GTGTGAGAAA
    GTTATAAAGT ACTTCATACC ATCCTTTGGT GGAAAGTCTT ACATGGCGTT CCAGACCATG
    AAGGCCTACC ATACTGTACG CATTGCCATG GAGTTCAGGG CTTCGGAAAT GACTGGGATA
    TTGCTCTACA ATGGGCAAAA AAGCAAAAAG GACTTTCTTT CTCTGACTCT AGTTGAAGGA
    TATGTGGAGC TCAGGTTTAA CACTGGTTCA GGGACTGCTT CAGTTATCAG TAAAGTCCTG
    GTAAAGCCGG GTCGCTGGCA TCAGCTTGTT GTAGTACGTA ACAGGCGCAA TGCCATGCTG
    AGCGTGGACA ATGAGCCCAG CGTTGAAGGA CAAAGTCCTC CAGGAACAGA TGGACTCAAC
    CTGGACACAG ACCTTTTCAT CGGCGGAGTG CTTGATGACA TGAGTGCAGA CGTAAAGGAG
    AGGACATCAG TTTCAGCAGG TCTGGTGGGC TGCATCCGCA TGCTGGACGT GAACAACCTT
    GTCTATAATC TGCAAGAGAA TGGCGGCAGC GTTCTGTACG GTTCGGCCAT TGGCGAGTGT
    GGCAACAACC CATGCCTGCC AAACCCCTGC AAGAACGGAG CGTCCTGTCA GGTCAAAGAA
    GCCGAGATTT TCCACTGCAA ATGTGTCAAC GGTTTCTCTG GCCCGACCTG CGCTGATGCC
    CACAATCCCT GCGACCCCAA TAAATGCCAC CCGCCATCAC GGTGCCAGGT CCTTCCAGAG
    GGAGGTTACA AATGCGAATG TCCGATGGGA CGTGAAGGCA GGCACTGTGA AAAAGTGTCA
    GACAAGGGAG GCGCTTTCAT TCCTTACTTC ACTGGAGATT CTTTTCTGGA GCTCAAAGGC
    CTCCATACGT ATAACCAGGA TCTCAGGCAA AAATTCAGCA TGACTATCGT TCTACTGGCC
    AATGACTCCA AAGGCATGAT CTTCTACAAT GGTCAGAAGA CAGACGGCAA AGGGGATTTC
    ATCTCGCTCT CTCTTAATGA CGGCATCCTG GAGTTTAGAT ATGACCTGGG CAAAGGGCCA
    GCTGTCATTA GGAGTAAAGA AAAAATCAAG CTGAACGAGT GGAACACAGT GAATTTGGAA
    CGGGCCAGCA GAAAAGGAGA GATTAGCATT AACGGCAAAG ACCCTGTCAG AGGAGAGGCG
    CCGAAATCAC GCAAGAATCA GCACACGGAT CTCAATCTGA AGGAATCGCT CTTTGTGGGT
    GGAGCTCCTG ATTTCCGCAA AGTAGCCAGA GCTGCTGCGA TCAAAGATGG CTTCAAAGGG
    GCCATTCAGA AGATTACTTT GATGGGAATT CCAATCCTGA AAGCTGATAA TGCCCTTTCG
    TCCACTGACG TGTCCATGTA TCCTAATCAT CCCTGCTCCA AAGACGTTTG TGAGAATGGA
    GGCCGCTGCA ACCCCCAGCT GAACAGCTAT GAGTGCGCCT GCCGCCACGG ATTCTCCGGA
    CACCACTGCC AGGACACAAT ATTTGAGAAG TCCGCTGGGG ACACGGAGGC CATTGCGTTT
    GATGGTCATA CATTCATCGA ATATCACAAC GGAGTCACCA AAAGCCCTGA GTCACTGGAG
    AACCCATCTG ACCAGAGCGA GAAAGCCCTG CTGGTGAATA AATTCGAGCT GAGCATCAGG
    ACGGAGGCTA CGCATGGTCT GATTTTGTGG AGCGGAAAGG GAGTTGAGCG CTCTGACTAC
    ATCGCCCTCG CCATTGTGGA TGGAAGAGTA CAGATGACCT ATGACCTCGG CTCCAAGCCT
    GTGGTGCTGC GCTCCACTGT ACGGGTCAAC ACCAACCACT GGATACGAAT TAAGGCCAGC
    AGAGCGCTCA GAGATGGCTC TCTGCAGGTT GGTAATGAGG CAGCTGTCAC TGGGTCTTCA
    CCCTTGGCTG CCACACAGCT GGACACAGAC GGGGCTCTCT GGTTGGGTGG ACTGGAGGAG
    CTGTCTGTGG CTCGACGGCT GCCCAAAGCT TACAGTACAG GCTTCATTGG CTGCGTCAAA
    GACGTGATAG TGGACGGCGT GGAGCTGCAT CTGGTGGAGG ACGCCTTAAA TACTCCCAAA
    ATATCACACT GTTCTGCCAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa33967
    Clone ID Related Accession (Same CDS sequence) NM_001177452.1
    Accession Version NM_001177452.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6087bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-09
    Organism Danio rerio(zebrafish)
    Product agrin precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU716327.1. On Apr 26, 2010 this sequence version replaced XM_001920222.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU716327.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705, SAMN06350654 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA AAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGGAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATAAACC TGTTCACTTC
    ACACCGGCAC CTCCTCCTGA TGGTTGCAGA GGGAAGTTGT GCGGATTTGG AGCGGTGTGT
    GAGAGAGACC AAGCTGACCC ATCTAAAGGA GAGTGTGTGT GTAAGAAGAT TGTGTGCACA
    TCTGTGGTGG CGCCAGTGTG CGGATCTGAC TCCTCCACCT ATTCCAATGA GTGTGAGCTG
    GAGAAGGCTC AGTGCAATAC ACAGAGACGC ATCAAGGTGA TGCGCAAAGG CCCCTGCGCC
    CTCAAGGACC CGTGCAGTGA GGTAACCTGT AGCTTTGGCA GCACCTGCAT CCAGTCATCG
    GATGGTCTGT CCGCCAAGTG TATGTGTCCT CTGAGCTGTG AAAACGTCCC CAAGCATGTG
    GTGTGTGGCA GTGACGGCCT GGACTACCAA AGCGAATGTG AACTCAACAT GAAGGCCTGC
    GCCACTCAGA AGAACATCCG TGTGCATCAC CAGGGCCGCT GCGATCCTTG CAGTGACACT
    CTGAACAGCT TAAGCACAGC CTGTCGAGTC AACCCAAAGA CCTACCAGCA GATTACCTTT
    GCCCCGGCTG AGTCTTGTCC GCCTGACGAA ATGCCCATCT GTGCCAGCGA CGGACACACC
    TACGAGAGCA TGTGTCAGAT GGAGCGCACT GCCCTGAAGA ACAACCTGGA GCTCAAAAAG
    GTCTCTTTAG GCAGCTGCAA GGAAAAAGGG GGCTGCCCGA ATGGTTGTAA GTTCAACGCC
    ATATGTTTGC TGGATAATGG AGAGTTTCGG TGCTCCTGCG ATCCCATACA ATGCGACGGC
    ACGTACAAGC CCCTGTGCGG AAAGAATGGC AAGACATACC CCAACGACTG CGAGAGGAAA
    CTGCAGGAAT GCCGGACCCA AAAAGACATC CCTGTCAAAC AACAAGGGCC CTGTGATCTT
    AACCTGGAGA GCCCCTGCCT GAAGAAGACC TGCGAGTTTG GAGCCCTGTG CGTGGTGAAG
    AACAGCGAAG CAGTGTGTGA ATGCTCAGAC GCCTGTCCGC AGGACCAAGA CCCTGTTTGC
    GGCAGCGATG GGCACACGTA CAGCAGCTCA TGTCAAATGA AGGCCATGGG ATGCGCTCTT
    CAGAAACAGA TACAGATGCA ACACAAAGGA CCATGTGCAG ATGAGTCCCG CACCAACTGC
    TCGTTTGGCG CGATCTGTGA TGCCCAGACT GGCCGGTGCG TGTGCCCTAA AGGCTGTTTG
    GAAACACGGC AGCCCGTATG CGGCAGCGAC GGCACCACGT ATGACAACGA GTGCAAGCTC
    AACGTCCAAG CCTGCACTAA ACAGCTGGAT CTCAAGGTGG TGGCCCATGG AGAATGCAAA
    ACCTGTGGCA GCACTGTGTG TAGCTGGGGG TCGCAGTGCG TGAACAACAA GTGTGAGTGT
    CAGCAGTGCA CAGGTCAGCC GGTGAAACCT GTGTGTGGCA GCGACGGCAA CACATACAAC
    AACGACTGCG AGCTGAGACT GGCCTCCTGT CAGAAACAGA GGAAGATAGA GGTGGCCAAG
    CCTGGCGTCT GCGATGAAGA CTGTGGTTCA GGAGGCTCCG GCTCGGGATC TGAGACTTGT
    GAACAGGAAC GCTGTCGCCG ATATGGAGGA ACGTGGGACG ATGACATGGA GGACGAGCGA
    TGTGTTTGTG AACTAAACTG TCAGATTGTG CTGCACAGCC CGGTGTGCGG CTCTGATGGA
    AACACGTACT CCAGCGACTG TGAGCTCAAG AAGACCAGGT GCGAAAAGCA AATAGACCTG
    AGTGTCCAAA GCCAAGGGCC CTGTCCAACT ATCGTATCAC CACCTCCAGA GCTTGTTGCC
    TCCCAGCACT GTAGCCAAAC AGTTTATGGA TGTTGTGAAG ATAACAAGAC CGCTGCCCTG
    GGTGTTGGAC TGGCTGGATG TCCTAGTATG TGTCAGTGCA ATCATTACGG CTCATACGGA
    GGCACATGTG ACCCCTCGAC TGGCCAGTGT TCCTGTAAAC CTGGTGTCGG TGGACTGAAG
    TGCGACCGCT GTGAGCCCGG ATTTTGGAAC TTCCGTGGCA TTGTCACAGA AAACATGAGC
    GGCTGCACAC CGTGCAACTG TGATGGACTG GGTTCGGTTC GTGATGACTG CGAGCAGATG
    TCAGGCCTGT GCTCCTGTAA GCCTGGAGTT AAGGGCATGA AATGCAACGT GTGCCCTGAT
    GGCAGCAAGA TGGGAGTCAA CGGCTGTGAC AAAGGACCTA AGGCTCCCAA GTCCTGTGCT
    GAGCTTGTGT GCCACTTTGG GTCTTCATGC GTGGAGGTGA ATGGACAGGC TCACTGTGAG
    TGTCCTTCAC CAGATTGTGA TGAGAAAAAC AAGACCAAGG TATGTGGTTC TGATGGGGTC
    ACCTATGCAG ACCGGTGCCA GCTGAAGACC ATCGCATGCC GACAAGATAA AGAGATCAAG
    GTGGAGCATT TAGGCCAGTG TAAAGAATCC ATTGAGCTGA CGGCCAAACC CACCCCCTTC
    CCAACCACCC ACCGCACCAC CCCTCCCACC GCTCACGTCA AGGTCACCAA ACAGCGACAT
    GGCCACCGCA CCACCACTCC GGACAGCTAT GTGGTGGAGG CAATGCCTCC ACCGAGGTTG
    GATGGAGCGC GAAAGTTTTC CAGCCCTTTC ACCACCCACC GTCCCACTAC ACCCTACAAA
    CCACTCCGCA CCTCATCCCC ACCTCTGGAC TTTGACGAGT CGGGCAGTGG AGAGCCCAGT
    GGGGATGATG AGATGGTGAG CGGAATGGAG GAGAGCGGAG AAGAGCCACT TGGTACAACA
    GTAGCTACTA CCACTCTTCC CACAGCTGAG GAGCGATCGT CCTGTGACAA CACAGCGTTC
    GGCTGCTGTC CCGATGGGAA GATGGCCGCC GTCAACTCAG AGGGCACAAA CTGCCCATCC
    ACCATGCGTT TCAGCGGCTT CCTGCACCTG GATAAGGTTG AGGGTCAGGA GGTCTTCTAC
    ACGCCTGAGA TGGAGGACCC CAAATCGGAA CTGTTTGGTG AAACGGCCCG AAGCATCGAG
    AGCGCTATGA ATGAGTTGTT CCGCAAGTCT GATGTCCAGA AAGACTTCCA GAGTGTCCAT
    GTGAGGAATC TATCCCCCAG CAACTCCATA ATAGCACATA TCGAGGCTCA CTTCGACCCA
    GACACAAGGT TCAATGTGGG GGATATTGAG GGAGCCCTTC TCAAACAGCT TAAGGCATCT
    AAAGACACTG GCATCGTTGT GAAGAAACCG GAGGAAGAGA ACATTCACAT CAACAACTAT
    GGTCTTTCAA CTGTTCCTTT CTTTACCACC ACAACAACCA CTGTGGCGTC TGTCACGACT
    CCCACTACCA CCACCACCAC AATGTCTCCC AGTTCTGCTT ATACCACTCT CCGGCCCATC
    ACTACTAGAC GACCCTTCAC AAGCCACAGA ACCACCACGC CAGCACTAGC CACCACCACT
    GCACCACATG TGCCCAAGAC CACAGTTTCG CTTATGAACA GACTAGGGCC CCACGCTCAC
    AGTCCATGTG ACTCACAGCC CTGCCGACAT GGAGGAACCT GTGAAGAAGA CGACAATGAC
    TTCACCTGTA TCTGTCCAGC AGGACGAGGA GGAGCCGTGT GTGAGAAAGT TATAAAGTAC
    TTCATACCAT CCTTTGGTGG AAAGTCTTAC ATGGCGTTCC AGACCATGAA GGCCTACCAT
    ACTGTACGCA TTGCCATGGA GTTCAGGGCT TCGGAAATGA CTGGGATATT GCTCTACGAT
    GGGCAAAAAA GCAAAAAGGA CTTTCTTTCT CTGACACTTA TTGAAGGATA TGTGGAGCTC
    AGGTTTAACA CTGGTTCAGG GACTGCTTCA GTTATCAGTA AAGTCCTGGT AAAGCCGGGT
    CGCTGGCATC AGCTTGTTGT AGTACGTAAC AGGCGCAATG CCATGCTGAG CGTGGACAAT
    GAGCCCAGCG TTGAAGGACA AAGTCCTCCA GGAACAGATG GACTCAACCT GGACACAGAC
    CTTTTCATCG GCGGAGTGCT TGATGACATG AGTGCAGACG TAAAGGAGAG GACATCAGTT
    TCAGCAGGTC TGGTGGGCTG CATCCGCATG CTGGACGTGA ACAACCTTGT CTATAATCTG
    CAAGAGAATG GCGGCAGCGT TCTGTACGGT TCGGCCATTG GCGAGTGTGG CAACAACCCA
    TGCCTGCCAA ACCCCTGCAA GAACGGAGCG TCCTGTCAGG TCAAAGAAGC CGAGATTTTC
    CACTGCAAAT GTGTCAACGG TTTCTCTGGC CCGACCTGCG CTGATGCCCA CAACCCCTGC
    GACCCCAATA AATGCCACCC GCCATCACGG TGCCAGGTCC TTCCAGAGGG AGGTTACAAA
    TGCGAATGTC CGATGGGACG TGAAGGCAGG CACTGTGAAA AAGTGTCAGA CAAGGGAGGC
    GCTTTCATTC CTTACTTCAC TGGAGATTCT TTTCTGGAGC TCAAAGGCCT CCATACGTAT
    AACCAGGATC TCAGGCAAAA ATTCAGCATG ACTATCGTTC TACTGGCCAA TGACTCCAAA
    GGCATGATCT TCTACAATGG TCAGAAGACA GACGGCAAAG GGGATTTCAT CTCGCTCTCT
    CTTAATGACG GCATCCTGGA GTTTAGATAT GACCTGGGCA AAGGGCCAGC TGTCATTAGG
    AGTAAAGAAA AAATCAAGCT GAACGAGTGG AACACAGTGA ATTTGGAACG GGCCAGCAGA
    AAAGGAGAGA TTAGCATTAA CGGCAAAGAC CCTGTCAGAG GAGAGGCGCC GAATCAGCAC
    ACGGATCTCA ATCTGAAGGA ATCGCTCTTT GTGGGTGGAG CTCCTGATTT CCGCAAAGTA
    GCCAGAGCTG CTGCGTTCAA AGATGGCTTC AAAGGGGCCA TTCAGAAGAT TACTTTGATG
    GGAATTCCAA TCCTGAAAGC TGATAATGCC CTTTCGTCCA CTGACGTGTC CATGTATCCT
    AATCATCCCT GCTCCAAAGA CGTTTGTGAG AATGGAGGCC GCTGCAACCC CCAGCTGAAC
    AGCTATGAGT GCGCCTGCCG CCACGGATTC TCCGGACACC ACTGCCAGGA CACAATATTC
    GAGAAGTCCG CTGGGGACAC GGAGGCCATT GCGTTTGATG GTCATACATT CATCGAATAT
    CACAACGGAG TCACCAAAAG CGAGAAAGCC CTGCTGGTGA ATAAATTCGA GCTGAGTATC
    AGGACGGAGG CTACGCATGG TCTGATTTTG TGGAGCGGAA AGGGAGTTGA GCGCTCTGAC
    TACATCGCCC TCGCCATTGT GGATGGAAGA GTACAGATGA CCTATGACCT CGGCTCCAAG
    CCTGTGGTGC TGCGCTCCAC TGTACGGGTC AACACCAACC ACTGGATACG AATTAAGGCC
    AGCAGAGCGC TCAGAGATGG CTCTCTGCAG GTTGGTAATG AGGCAGCTGT CACTGGGTCT
    TCACCCTTGG CTGCCACACA GCTGGACACA GACGGGGCTC TCTGGTTGGG TGGACTGGAG
    GAGCTGTCTG TGGCTCGACG GCTGCCCAAA GCTTACAGTA CAGGCTTCAT TGGCTGCGTC
    AAAGACGTGA TAGTGGACGG CGTGGAGCTG CATCTGGTGG AGGACGCCTT AAATACTCCC
    AAAATATCAC ACTGTTCTGC CAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001170923.1
    CDS159..6245
    Translation

    Target ORF information:

    RefSeq Version NM_001177452.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio agrin (agrn), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177452.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA AAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGGAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATAAACC TGTTCACTTC
    ACACCGGCAC CTCCTCCTGA TGGTTGCAGA GGGAAGTTGT GCGGATTTGG AGCGGTGTGT
    GAGAGAGACC AAGCTGACCC ATCTAAAGGA GAGTGTGTGT GTAAGAAGAT TGTGTGCACA
    TCTGTGGTGG CGCCAGTGTG CGGATCTGAC TCCTCCACCT ATTCCAATGA GTGTGAGCTG
    GAGAAGGCTC AGTGCAATAC ACAGAGACGC ATCAAGGTGA TGCGCAAAGG CCCCTGCGCC
    CTCAAGGACC CGTGCAGTGA GGTAACCTGT AGCTTTGGCA GCACCTGCAT CCAGTCATCG
    GATGGTCTGT CCGCCAAGTG TATGTGTCCT CTGAGCTGTG AAAACGTCCC CAAGCATGTG
    GTGTGTGGCA GTGACGGCCT GGACTACCAA AGCGAATGTG AACTCAACAT GAAGGCCTGC
    GCCACTCAGA AGAACATCCG TGTGCATCAC CAGGGCCGCT GCGATCCTTG CAGTGACACT
    CTGAACAGCT TAAGCACAGC CTGTCGAGTC AACCCAAAGA CCTACCAGCA GATTACCTTT
    GCCCCGGCTG AGTCTTGTCC GCCTGACGAA ATGCCCATCT GTGCCAGCGA CGGACACACC
    TACGAGAGCA TGTGTCAGAT GGAGCGCACT GCCCTGAAGA ACAACCTGGA GCTCAAAAAG
    GTCTCTTTAG GCAGCTGCAA GGAAAAAGGG GGCTGCCCGA ATGGTTGTAA GTTCAACGCC
    ATATGTTTGC TGGATAATGG AGAGTTTCGG TGCTCCTGCG ATCCCATACA ATGCGACGGC
    ACGTACAAGC CCCTGTGCGG AAAGAATGGC AAGACATACC CCAACGACTG CGAGAGGAAA
    CTGCAGGAAT GCCGGACCCA AAAAGACATC CCTGTCAAAC AACAAGGGCC CTGTGATCTT
    AACCTGGAGA GCCCCTGCCT GAAGAAGACC TGCGAGTTTG GAGCCCTGTG CGTGGTGAAG
    AACAGCGAAG CAGTGTGTGA ATGCTCAGAC GCCTGTCCGC AGGACCAAGA CCCTGTTTGC
    GGCAGCGATG GGCACACGTA CAGCAGCTCA TGTCAAATGA AGGCCATGGG ATGCGCTCTT
    CAGAAACAGA TACAGATGCA ACACAAAGGA CCATGTGCAG ATGAGTCCCG CACCAACTGC
    TCGTTTGGCG CGATCTGTGA TGCCCAGACT GGCCGGTGCG TGTGCCCTAA AGGCTGTTTG
    GAAACACGGC AGCCCGTATG CGGCAGCGAC GGCACCACGT ATGACAACGA GTGCAAGCTC
    AACGTCCAAG CCTGCACTAA ACAGCTGGAT CTCAAGGTGG TGGCCCATGG AGAATGCAAA
    ACCTGTGGCA GCACTGTGTG TAGCTGGGGG TCGCAGTGCG TGAACAACAA GTGTGAGTGT
    CAGCAGTGCA CAGGTCAGCC GGTGAAACCT GTGTGTGGCA GCGACGGCAA CACATACAAC
    AACGACTGCG AGCTGAGACT GGCCTCCTGT CAGAAACAGA GGAAGATAGA GGTGGCCAAG
    CCTGGCGTCT GCGATGAAGA CTGTGGTTCA GGAGGCTCCG GCTCGGGATC TGAGACTTGT
    GAACAGGAAC GCTGTCGCCG ATATGGAGGA ACGTGGGACG ATGACATGGA GGACGAGCGA
    TGTGTTTGTG AACTAAACTG TCAGATTGTG CTGCACAGCC CGGTGTGCGG CTCTGATGGA
    AACACGTACT CCAGCGACTG TGAGCTCAAG AAGACCAGGT GCGAAAAGCA AATAGACCTG
    AGTGTCCAAA GCCAAGGGCC CTGTCCAACT ATCGTATCAC CACCTCCAGA GCTTGTTGCC
    TCCCAGCACT GTAGCCAAAC AGTTTATGGA TGTTGTGAAG ATAACAAGAC CGCTGCCCTG
    GGTGTTGGAC TGGCTGGATG TCCTAGTATG TGTCAGTGCA ATCATTACGG CTCATACGGA
    GGCACATGTG ACCCCTCGAC TGGCCAGTGT TCCTGTAAAC CTGGTGTCGG TGGACTGAAG
    TGCGACCGCT GTGAGCCCGG ATTTTGGAAC TTCCGTGGCA TTGTCACAGA AAACATGAGC
    GGCTGCACAC CGTGCAACTG TGATGGACTG GGTTCGGTTC GTGATGACTG CGAGCAGATG
    TCAGGCCTGT GCTCCTGTAA GCCTGGAGTT AAGGGCATGA AATGCAACGT GTGCCCTGAT
    GGCAGCAAGA TGGGAGTCAA CGGCTGTGAC AAAGGACCTA AGGCTCCCAA GTCCTGTGCT
    GAGCTTGTGT GCCACTTTGG GTCTTCATGC GTGGAGGTGA ATGGACAGGC TCACTGTGAG
    TGTCCTTCAC CAGATTGTGA TGAGAAAAAC AAGACCAAGG TATGTGGTTC TGATGGGGTC
    ACCTATGCAG ACCGGTGCCA GCTGAAGACC ATCGCATGCC GACAAGATAA AGAGATCAAG
    GTGGAGCATT TAGGCCAGTG TAAAGAATCC ATTGAGCTGA CGGCCAAACC CACCCCCTTC
    CCAACCACCC ACCGCACCAC CCCTCCCACC GCTCACGTCA AGGTCACCAA ACAGCGACAT
    GGCCACCGCA CCACCACTCC GGACAGCTAT GTGGTGGAGG CAATGCCTCC ACCGAGGTTG
    GATGGAGCGC GAAAGTTTTC CAGCCCTTTC ACCACCCACC GTCCCACTAC ACCCTACAAA
    CCACTCCGCA CCTCATCCCC ACCTCTGGAC TTTGACGAGT CGGGCAGTGG AGAGCCCAGT
    GGGGATGATG AGATGGTGAG CGGAATGGAG GAGAGCGGAG AAGAGCCACT TGGTACAACA
    GTAGCTACTA CCACTCTTCC CACAGCTGAG GAGCGATCGT CCTGTGACAA CACAGCGTTC
    GGCTGCTGTC CCGATGGGAA GATGGCCGCC GTCAACTCAG AGGGCACAAA CTGCCCATCC
    ACCATGCGTT TCAGCGGCTT CCTGCACCTG GATAAGGTTG AGGGTCAGGA GGTCTTCTAC
    ACGCCTGAGA TGGAGGACCC CAAATCGGAA CTGTTTGGTG AAACGGCCCG AAGCATCGAG
    AGCGCTATGA ATGAGTTGTT CCGCAAGTCT GATGTCCAGA AAGACTTCCA GAGTGTCCAT
    GTGAGGAATC TATCCCCCAG CAACTCCATA ATAGCACATA TCGAGGCTCA CTTCGACCCA
    GACACAAGGT TCAATGTGGG GGATATTGAG GGAGCCCTTC TCAAACAGCT TAAGGCATCT
    AAAGACACTG GCATCGTTGT GAAGAAACCG GAGGAAGAGA ACATTCACAT CAACAACTAT
    GGTCTTTCAA CTGTTCCTTT CTTTACCACC ACAACAACCA CTGTGGCGTC TGTCACGACT
    CCCACTACCA CCACCACCAC AATGTCTCCC AGTTCTGCTT ATACCACTCT CCGGCCCATC
    ACTACTAGAC GACCCTTCAC AAGCCACAGA ACCACCACGC CAGCACTAGC CACCACCACT
    GCACCACATG TGCCCAAGAC CACAGTTTCG CTTATGAACA GACTAGGGCC CCACGCTCAC
    AGTCCATGTG ACTCACAGCC CTGCCGACAT GGAGGAACCT GTGAAGAAGA CGACAATGAC
    TTCACCTGTA TCTGTCCAGC AGGACGAGGA GGAGCCGTGT GTGAGAAAGT TATAAAGTAC
    TTCATACCAT CCTTTGGTGG AAAGTCTTAC ATGGCGTTCC AGACCATGAA GGCCTACCAT
    ACTGTACGCA TTGCCATGGA GTTCAGGGCT TCGGAAATGA CTGGGATATT GCTCTACGAT
    GGGCAAAAAA GCAAAAAGGA CTTTCTTTCT CTGACACTTA TTGAAGGATA TGTGGAGCTC
    AGGTTTAACA CTGGTTCAGG GACTGCTTCA GTTATCAGTA AAGTCCTGGT AAAGCCGGGT
    CGCTGGCATC AGCTTGTTGT AGTACGTAAC AGGCGCAATG CCATGCTGAG CGTGGACAAT
    GAGCCCAGCG TTGAAGGACA AAGTCCTCCA GGAACAGATG GACTCAACCT GGACACAGAC
    CTTTTCATCG GCGGAGTGCT TGATGACATG AGTGCAGACG TAAAGGAGAG GACATCAGTT
    TCAGCAGGTC TGGTGGGCTG CATCCGCATG CTGGACGTGA ACAACCTTGT CTATAATCTG
    CAAGAGAATG GCGGCAGCGT TCTGTACGGT TCGGCCATTG GCGAGTGTGG CAACAACCCA
    TGCCTGCCAA ACCCCTGCAA GAACGGAGCG TCCTGTCAGG TCAAAGAAGC CGAGATTTTC
    CACTGCAAAT GTGTCAACGG TTTCTCTGGC CCGACCTGCG CTGATGCCCA CAACCCCTGC
    GACCCCAATA AATGCCACCC GCCATCACGG TGCCAGGTCC TTCCAGAGGG AGGTTACAAA
    TGCGAATGTC CGATGGGACG TGAAGGCAGG CACTGTGAAA AAGTGTCAGA CAAGGGAGGC
    GCTTTCATTC CTTACTTCAC TGGAGATTCT TTTCTGGAGC TCAAAGGCCT CCATACGTAT
    AACCAGGATC TCAGGCAAAA ATTCAGCATG ACTATCGTTC TACTGGCCAA TGACTCCAAA
    GGCATGATCT TCTACAATGG TCAGAAGACA GACGGCAAAG GGGATTTCAT CTCGCTCTCT
    CTTAATGACG GCATCCTGGA GTTTAGATAT GACCTGGGCA AAGGGCCAGC TGTCATTAGG
    AGTAAAGAAA AAATCAAGCT GAACGAGTGG AACACAGTGA ATTTGGAACG GGCCAGCAGA
    AAAGGAGAGA TTAGCATTAA CGGCAAAGAC CCTGTCAGAG GAGAGGCGCC GAATCAGCAC
    ACGGATCTCA ATCTGAAGGA ATCGCTCTTT GTGGGTGGAG CTCCTGATTT CCGCAAAGTA
    GCCAGAGCTG CTGCGTTCAA AGATGGCTTC AAAGGGGCCA TTCAGAAGAT TACTTTGATG
    GGAATTCCAA TCCTGAAAGC TGATAATGCC CTTTCGTCCA CTGACGTGTC CATGTATCCT
    AATCATCCCT GCTCCAAAGA CGTTTGTGAG AATGGAGGCC GCTGCAACCC CCAGCTGAAC
    AGCTATGAGT GCGCCTGCCG CCACGGATTC TCCGGACACC ACTGCCAGGA CACAATATTC
    GAGAAGTCCG CTGGGGACAC GGAGGCCATT GCGTTTGATG GTCATACATT CATCGAATAT
    CACAACGGAG TCACCAAAAG CGAGAAAGCC CTGCTGGTGA ATAAATTCGA GCTGAGTATC
    AGGACGGAGG CTACGCATGG TCTGATTTTG TGGAGCGGAA AGGGAGTTGA GCGCTCTGAC
    TACATCGCCC TCGCCATTGT GGATGGAAGA GTACAGATGA CCTATGACCT CGGCTCCAAG
    CCTGTGGTGC TGCGCTCCAC TGTACGGGTC AACACCAACC ACTGGATACG AATTAAGGCC
    AGCAGAGCGC TCAGAGATGG CTCTCTGCAG GTTGGTAATG AGGCAGCTGT CACTGGGTCT
    TCACCCTTGG CTGCCACACA GCTGGACACA GACGGGGCTC TCTGGTTGGG TGGACTGGAG
    GAGCTGTCTG TGGCTCGACG GCTGCCCAAA GCTTACAGTA CAGGCTTCAT TGGCTGCGTC
    AAAGACGTGA TAGTGGACGG CGTGGAGCTG CATCTGGTGG AGGACGCCTT AAATACTCCC
    AAAATATCAC ACTGTTCTGC CAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44574
    Clone ID Related Accession (Same CDS sequence) XM_009296860.3 , XM_009296860.2
    Accession Version XM_009296860.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6147bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product agrin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296860.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAAAT CACGCAAGAA TCAGCACACG GATCTCAATC TGAAGGAATC GCTCTTTGTG
    GGTGGAGCTC CTGATTTCCG CAAAGTAGCC AGAGCTGCTG CGATCAAAGA TGGCTTCAAA
    GGGGCCATTC AGAAGATTAC TTTGATGGGA ATTCCAATCC TGAAAGCTGA TAATGCCCTT
    TCGTCCACTG ACGTGTCCAT GTATCCTAAT CATCCCTGCT CCAAAGACGT TTGTGAGAAT
    GGAGGCCGCT GCAACCCCCA GCTGAACAGC TATGAGTGCG CCTGCCGCCA CGGATTCTCC
    GGACACCACT GCCAGGACAC AATATTTGAG AAGTCCGCTG GGGACACGGA GGCCATTGCG
    TTTGATGGTC ATACATTCAT CGAATATCAC AACGGAGTCA CCAAAAGCCC TGAGTCACTG
    GAGAACCCAT CTGACCAGAG CGAGAAAGCC CTGCTGGTGA ATAAATTCGA GCTGAGCATC
    AGGACGGAGG CTACGCATGG TCTGATTTTG TGGAGCGGAA AGGGAGTTGA GCGCTCTGAC
    TACATCGCCC TCGCCATTGT GGATGGAAGA GTACAGATGA CCTATGACCT CGGCTCCAAG
    CCTGTGGTGC TGCGCTCCAC TGTACGGGTC AACACCAACC ACTGGATACG AATTAAGGCC
    AGCAGAGCGC TCAGAGATGG CTCTCTGCAG GTTGGTAATG AGGCAGCTGT CACTGGGTCT
    TCACCCTTGG CTGCCACACA GCTGGACACA GACGGGGCTC TCTGGTTGGG TGGACTGGAG
    GAGCTGTCTG TGGCTCGACG GCTGCCCAAA GCTTACAGTA CAGGCTTCAT TGGCTGCGTC
    AAAGACGTGA TAGTGGACGG CGTGGAGCTG CATCTGGTGG AGGACGCCTT AAATACTCCC
    AAAATATCAC ACTGTTCTGC CAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295135.1
    CDS266..6412
    Translation

    Target ORF information:

    RefSeq Version XM_009296860.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296860.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAAAT CACGCAAGAA TCAGCACACG GATCTCAATC TGAAGGAATC GCTCTTTGTG
    GGTGGAGCTC CTGATTTCCG CAAAGTAGCC AGAGCTGCTG CGATCAAAGA TGGCTTCAAA
    GGGGCCATTC AGAAGATTAC TTTGATGGGA ATTCCAATCC TGAAAGCTGA TAATGCCCTT
    TCGTCCACTG ACGTGTCCAT GTATCCTAAT CATCCCTGCT CCAAAGACGT TTGTGAGAAT
    GGAGGCCGCT GCAACCCCCA GCTGAACAGC TATGAGTGCG CCTGCCGCCA CGGATTCTCC
    GGACACCACT GCCAGGACAC AATATTTGAG AAGTCCGCTG GGGACACGGA GGCCATTGCG
    TTTGATGGTC ATACATTCAT CGAATATCAC AACGGAGTCA CCAAAAGCCC TGAGTCACTG
    GAGAACCCAT CTGACCAGAG CGAGAAAGCC CTGCTGGTGA ATAAATTCGA GCTGAGCATC
    AGGACGGAGG CTACGCATGG TCTGATTTTG TGGAGCGGAA AGGGAGTTGA GCGCTCTGAC
    TACATCGCCC TCGCCATTGT GGATGGAAGA GTACAGATGA CCTATGACCT CGGCTCCAAG
    CCTGTGGTGC TGCGCTCCAC TGTACGGGTC AACACCAACC ACTGGATACG AATTAAGGCC
    AGCAGAGCGC TCAGAGATGG CTCTCTGCAG GTTGGTAATG AGGCAGCTGT CACTGGGTCT
    TCACCCTTGG CTGCCACACA GCTGGACACA GACGGGGCTC TCTGGTTGGG TGGACTGGAG
    GAGCTGTCTG TGGCTCGACG GCTGCCCAAA GCTTACAGTA CAGGCTTCAT TGGCTGCGTC
    AAAGACGTGA TAGTGGACGG CGTGGAGCTG CATCTGGTGG AGGACGCCTT AAATACTCCC
    AAAATATCAC ACTGTTCTGC CAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44574
    Clone ID Related Accession (Same CDS sequence) XM_009296860.3 , XM_009296860.2
    Accession Version XM_009296860.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6147bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product agrin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296860.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAAAT CACGCAAGAA TCAGCACACG GATCTCAATC TGAAGGAATC GCTCTTTGTG
    GGTGGAGCTC CTGATTTCCG CAAAGTAGCC AGAGCTGCTG CGATCAAAGA TGGCTTCAAA
    GGGGCCATTC AGAAGATTAC TTTGATGGGA ATTCCAATCC TGAAAGCTGA TAATGCCCTT
    TCGTCCACTG ACGTGTCCAT GTATCCTAAT CATCCCTGCT CCAAAGACGT TTGTGAGAAT
    GGAGGCCGCT GCAACCCCCA GCTGAACAGC TATGAGTGCG CCTGCCGCCA CGGATTCTCC
    GGACACCACT GCCAGGACAC AATATTTGAG AAGTCCGCTG GGGACACGGA GGCCATTGCG
    TTTGATGGTC ATACATTCAT CGAATATCAC AACGGAGTCA CCAAAAGCCC TGAGTCACTG
    GAGAACCCAT CTGACCAGAG CGAGAAAGCC CTGCTGGTGA ATAAATTCGA GCTGAGCATC
    AGGACGGAGG CTACGCATGG TCTGATTTTG TGGAGCGGAA AGGGAGTTGA GCGCTCTGAC
    TACATCGCCC TCGCCATTGT GGATGGAAGA GTACAGATGA CCTATGACCT CGGCTCCAAG
    CCTGTGGTGC TGCGCTCCAC TGTACGGGTC AACACCAACC ACTGGATACG AATTAAGGCC
    AGCAGAGCGC TCAGAGATGG CTCTCTGCAG GTTGGTAATG AGGCAGCTGT CACTGGGTCT
    TCACCCTTGG CTGCCACACA GCTGGACACA GACGGGGCTC TCTGGTTGGG TGGACTGGAG
    GAGCTGTCTG TGGCTCGACG GCTGCCCAAA GCTTACAGTA CAGGCTTCAT TGGCTGCGTC
    AAAGACGTGA TAGTGGACGG CGTGGAGCTG CATCTGGTGG AGGACGCCTT AAATACTCCC
    AAAATATCAC ACTGTTCTGC CAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295135.1
    CDS276..6422
    Translation

    Target ORF information:

    RefSeq Version XM_009296860.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296860.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAAAT CACGCAAGAA TCAGCACACG GATCTCAATC TGAAGGAATC GCTCTTTGTG
    GGTGGAGCTC CTGATTTCCG CAAAGTAGCC AGAGCTGCTG CGATCAAAGA TGGCTTCAAA
    GGGGCCATTC AGAAGATTAC TTTGATGGGA ATTCCAATCC TGAAAGCTGA TAATGCCCTT
    TCGTCCACTG ACGTGTCCAT GTATCCTAAT CATCCCTGCT CCAAAGACGT TTGTGAGAAT
    GGAGGCCGCT GCAACCCCCA GCTGAACAGC TATGAGTGCG CCTGCCGCCA CGGATTCTCC
    GGACACCACT GCCAGGACAC AATATTTGAG AAGTCCGCTG GGGACACGGA GGCCATTGCG
    TTTGATGGTC ATACATTCAT CGAATATCAC AACGGAGTCA CCAAAAGCCC TGAGTCACTG
    GAGAACCCAT CTGACCAGAG CGAGAAAGCC CTGCTGGTGA ATAAATTCGA GCTGAGCATC
    AGGACGGAGG CTACGCATGG TCTGATTTTG TGGAGCGGAA AGGGAGTTGA GCGCTCTGAC
    TACATCGCCC TCGCCATTGT GGATGGAAGA GTACAGATGA CCTATGACCT CGGCTCCAAG
    CCTGTGGTGC TGCGCTCCAC TGTACGGGTC AACACCAACC ACTGGATACG AATTAAGGCC
    AGCAGAGCGC TCAGAGATGG CTCTCTGCAG GTTGGTAATG AGGCAGCTGT CACTGGGTCT
    TCACCCTTGG CTGCCACACA GCTGGACACA GACGGGGCTC TCTGGTTGGG TGGACTGGAG
    GAGCTGTCTG TGGCTCGACG GCTGCCCAAA GCTTACAGTA CAGGCTTCAT TGGCTGCGTC
    AAAGACGTGA TAGTGGACGG CGTGGAGCTG CATCTGGTGG AGGACGCCTT AAATACTCCC
    AAAATATCAC ACTGTTCTGC CAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44575
    Clone ID Related Accession (Same CDS sequence) XM_009296861.2
    Accession Version XM_009296861.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6135bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product agrin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296861.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAATC AGCACACGGA TCTCAATCTG AAGGAATCGC TCTTTGTGGG TGGAGCTCCT
    GATTTCCGCA AAGTAGCCAG AGCTGCTGCG ATCAAAGATG GCTTCAAAGG GGCCATTCAG
    AAGATTACTT TGATGGGAAT TCCAATCCTG AAAGCTGATA ATGCCCTTTC GTCCACTGAC
    GTGTCCATGT ATCCTAATCA TCCCTGCTCC AAAGACGTTT GTGAGAATGG AGGCCGCTGC
    AACCCCCAGC TGAACAGCTA TGAGTGCGCC TGCCGCCACG GATTCTCCGG ACACCACTGC
    CAGGACACAA TATTTGAGAA GTCCGCTGGG GACACGGAGG CCATTGCGTT TGATGGTCAT
    ACATTCATCG AATATCACAA CGGAGTCACC AAAAGCCCTG AGTCACTGGA GAACCCATCT
    GACCAGAGCG AGAAAGCCCT GCTGGTGAAT AAATTCGAGC TGAGCATCAG GACGGAGGCT
    ACGCATGGTC TGATTTTGTG GAGCGGAAAG GGAGTTGAGC GCTCTGACTA CATCGCCCTC
    GCCATTGTGG ATGGAAGAGT ACAGATGACC TATGACCTCG GCTCCAAGCC TGTGGTGCTG
    CGCTCCACTG TACGGGTCAA CACCAACCAC TGGATACGAA TTAAGGCCAG CAGAGCGCTC
    AGAGATGGCT CTCTGCAGGT TGGTAATGAG GCAGCTGTCA CTGGGTCTTC ACCCTTGGCT
    GCCACACAGC TGGACACAGA CGGGGCTCTC TGGTTGGGTG GACTGGAGGA GCTGTCTGTG
    GCTCGACGGC TGCCCAAAGC TTACAGTACA GGCTTCATTG GCTGCGTCAA AGACGTGATA
    GTGGACGGCG TGGAGCTGCA TCTGGTGGAG GACGCCTTAA ATACTCCCAA AATATCACAC
    TGTTCTGCCA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295136.1
    CDS276..6410
    Translation

    Target ORF information:

    RefSeq Version XM_009296861.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296861.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAATC AGCACACGGA TCTCAATCTG AAGGAATCGC TCTTTGTGGG TGGAGCTCCT
    GATTTCCGCA AAGTAGCCAG AGCTGCTGCG ATCAAAGATG GCTTCAAAGG GGCCATTCAG
    AAGATTACTT TGATGGGAAT TCCAATCCTG AAAGCTGATA ATGCCCTTTC GTCCACTGAC
    GTGTCCATGT ATCCTAATCA TCCCTGCTCC AAAGACGTTT GTGAGAATGG AGGCCGCTGC
    AACCCCCAGC TGAACAGCTA TGAGTGCGCC TGCCGCCACG GATTCTCCGG ACACCACTGC
    CAGGACACAA TATTTGAGAA GTCCGCTGGG GACACGGAGG CCATTGCGTT TGATGGTCAT
    ACATTCATCG AATATCACAA CGGAGTCACC AAAAGCCCTG AGTCACTGGA GAACCCATCT
    GACCAGAGCG AGAAAGCCCT GCTGGTGAAT AAATTCGAGC TGAGCATCAG GACGGAGGCT
    ACGCATGGTC TGATTTTGTG GAGCGGAAAG GGAGTTGAGC GCTCTGACTA CATCGCCCTC
    GCCATTGTGG ATGGAAGAGT ACAGATGACC TATGACCTCG GCTCCAAGCC TGTGGTGCTG
    CGCTCCACTG TACGGGTCAA CACCAACCAC TGGATACGAA TTAAGGCCAG CAGAGCGCTC
    AGAGATGGCT CTCTGCAGGT TGGTAATGAG GCAGCTGTCA CTGGGTCTTC ACCCTTGGCT
    GCCACACAGC TGGACACAGA CGGGGCTCTC TGGTTGGGTG GACTGGAGGA GCTGTCTGTG
    GCTCGACGGC TGCCCAAAGC TTACAGTACA GGCTTCATTG GCTGCGTCAA AGACGTGATA
    GTGGACGGCG TGGAGCTGCA TCTGGTGGAG GACGCCTTAA ATACTCCCAA AATATCACAC
    TGTTCTGCCA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44576
    Clone ID Related Accession (Same CDS sequence) XM_009296862.2
    Accession Version XM_009296862.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6126bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product agrin isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296862.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATAAACC TGTTCACTTC
    ACACCGGCAC CTCCTCCTGA TGGTTGCAGA GGGAAGTTGT GCGGATTTGG AGCGGTGTGT
    GAGAGAGACC AAGCTGACCC ATCTAAAGGA GAGTGTGTGT GTAAGAAGAT TGTGTGTACA
    TCTGTGGTGG CGCCAGTGTG CGGATCTGAC TCCTCCACCT ATTCCAATGA GTGTGAGCTG
    GAGAAGGCTC AGTGCAATAC ACAGAGACGC ATCAAGGTGA TGCGCAAAGG CCCCTGCGCC
    CTCAAGGACC CGTGCAGTGA GGTAACCTGT AGCTTTGGCA GCACCTGCAT CCAGTCATCG
    GATGGTCTGT CCGCCAAGTG TATGTGTCCT CTGAGCTGTG AAAACGTCCC CAAGCATGTG
    GTGTGTGGCA GTGACGGCCT GGACTACCAA AGCGAATGTG AACTCAACAT GAAGGCCTGC
    GCCACTCAGA AGAACATCCG TGTGCATCAC CAGGGCCGCT GCGATCCTTG CAGTGACACT
    CTGAACAGCT TAAGCACAGC CTGTCGAGTC AACCCAAAGA CCTACCAGCA GATTACCTTT
    GCCCCGGCTG AGTCTTGTCC GCCTGACGAA ATGCCCATCT GTGCCAGCGA CGGACACACC
    TACGAGAGCA TGTGTCAGAT GGAGCGCACT GCCCTGAAGA ACAACCTGGA GCTCAAAAAG
    GTCTCTTTAG GCAGCTGCAA GGAAAAAGGG GGCTGCCCGA ATGGTTGTAA GTTCAACGCC
    ATATGTTTGC TGGATAATGG AGAGTTTCGG TGCTCCTGCG ATCCCATACA ATGCGACGGC
    ACGTACAAGC CCCTGTGCGG AAAGGATGGC AAGACATACC CCAACGACTG CGAGAGGAAA
    CTGCAGGAAT GCCGGACCCA AAAAGACATC CCTGTCAAAC AACAAGGGCC CTGTGATCTT
    AACCTGGAGA GCCCCTGCCT GAAGAAGACC TGCGAGTTTG GAGCCGTGTG CGTGGTGAAG
    AACAGCGAAG CAGTGTGTGA ATGCTCAGAC GCCTGTCCGC AGGACCAAGA CCCTGTTTGC
    GGCAGCGATG GGCACACGTA CAGCAGCTCA TGTCAAATGA AGGCCATGGG ATGCGCTCTT
    CAGAAACAGA TACAGATGCA ACACAAAGGA CCATGTGCAG ATGAGTCCTG CACCAACTGC
    TCGTTTGGCG CGATCTGTGA TGCCCAGACT GGCCGGTGCG TGTGCCCTAA AGGCTGTTTG
    GAAACACGGC AGCCCGTATG CGGCAGCGAC GGCACCACGT ATGACAACGA GTGCAAGCTC
    AACGTCCAAG CCTGCACTAA ACAGCTGGAT CTCAAGGTGG TGGCCCATGG AGAATGCAAA
    ACCTGTGGCA GCACTGTGTG TAGCTGGGGG TCGCAGTGCG TGAACAACAA GTGTGAGTGT
    CAGCAGTGCA CTGGTCAGCC GGTGAAACCC GTGTGTGGCA GCGACGGCAA CACATACAAC
    AACGACTGCG AGCTGAGACT GGCCTCCTGT CAGAAACAGA GGAAGATAGA GGTGGCCAAG
    CCTGGCGTCT GCGATGAAGA CTGTGGTTCA GGAGGCTCCG GCTCGGGATC TGAGACTTGT
    GAACAGGACC GATGTCGCCG ATATGGAGGA ACGTGGGACG ATGACATGGA GGACGAGCGA
    TGTGTTTGTG AACTAAACTG TCAGATTGTG CTGCACAGCC CGGTGTGCGG CTCTGATGGA
    AACACGTACT CCAGCGACTG TGAGCTCAAG AAGACCAGGT GCGAAAAGCA AATAGACCTG
    AGTGTCCAAA GCCAAGGGCC CTGTCCAACT ATCGTATCAC CACCTCCAGA GCTTGTTGCC
    TCCCAGCACT GTAGCCAAAC AGTTTATGGA TGTTGTGAAG ATAACAAGAC CGCTGCCCTG
    GGTGTTGGAC TGGCTGGATG TCCTAGTATG TGTCAGTGCA ATCATTACGG CTCATACGGA
    GGCACATGTG ACCCCTCGAC TGGCCAGTGT TCCTGTAAAC CTGGTGTCGG TGGACTGAAG
    TGCGACCGCT GTGAGCCCGG ATTTTGGAAC TTCCGTGGCA TTGTCACAGA AAACATGAGC
    GGCTGCACAC CGTGCAACTG TGATGGAGTG GGTTCGGTTC GTGATGACTG CGAGCAGATG
    TCAGGCCTGT GCTCCTGTAA GCCTGGAGTT AAGGGCATGA AATGCAACGT GTGCCCTGAT
    GGCAGCAAGA TGGGAGTCAA CGGCTGTGAC AAAGGACCTA AGGCTCCCAA GTCCTGTGCT
    GAGCTTGTGT GCCACTTTGG GTCTTCATGC GTGGAGGTGA ATGGACAGGC TCACTGTGAG
    TGTCCTTCAC CAGATTGTGA TGAGAAAAAC AAGACCAAGG TATGTGGTTC TGATGGGGTC
    ACCTATGCAG ACCGGTGCCA GCTGAAGACC ATCGCATGCC GACAAGATAA AGAGATCAAG
    GTGGAGCATT TAGGCCAGTG TAAAGAATCC ATTGAGCTGA CGGCCAAACC CACCCCCTTC
    CCAACCACCC ACCGCACCAC CCCTCCCACC GCTCACGTCA AGGTCACCAA ACAGCGACAT
    GGCCACCGCA CCACCACTCC GGACAGCTAT GTGGTGGAGG CAATGCCTCC ACCGAGGTTG
    GATGGAGCGC GAAAGTTTTC CAGCCCTTTC ACCACCCACC GTCCCACTAC ACCCTACAAA
    CCACTCCGCA CCTCATCCCC ACCTCTGGAC TTTGACGAGT CGGGCAGTGG AGAGCCCAGT
    GGGGATGATG AGATGGTGAG CGGAATGGAG GAGAGCGGAG AAGAGCCACT TGGTACAACA
    GTAGCTACTA CCACTCTTCC CACAGCTGAG GAGCGATCGT CCTGTGACAA CACAGCGTTC
    GGCTGCTGTC CCGATGGGAA GACGGCCGCC GTCAACTCAG AGGGCACAAA CTGCCCATCC
    ACCATGCGTT TCAGCGGCTT CCTGCACCTG GATAAGGTTG AGGGTCAGGA GGTCTTCTAC
    ACGCCTGAGA TGGAGGACCC CAAATCGGAA CTGTTTGGTG AAACGGCCCG AAGCATCGAG
    AGCGCTATGA ATGAGTTGTT CCGCAAGTCT GATGTCCAGA AAGACTTCCA GAGTGTCCGT
    GTGAGGAATC TATCCCCCAG CAACTCCATA ATAGCACATT TCGAGGCTCA CTTCGACCCA
    GACACAAGGT TCAATGTGGG GGATATTGAG GGAGCCCTTC TCAAACAGCT TAAGGCGTCT
    AAAGACACTG GCATCGTTGT GAAGAAACCA GAGGAAGAGA ACATTCGCAT CAACAACTAT
    GGTCTTTCAA CTGTTCCTTT CTTTACCACC ACAACAACCA CTGTGGCATC TGTCACGACT
    CCCACTACCA CCACCACCAC AATGTCTCCC AGTTCTGCTT ATACCACTCT CCGGCCCATC
    ACTACTAGAC GACCCTTCAC AAGAACCACC ACGCCAGCAC CAGCCACCAC CACTGCACCA
    CATGTGCCCA AGACCACAGT TTCGCTTATG AACAGACTAG GGCCCCACGC TCACAGTCCA
    TGTGACTCTC AGCCCTGCCG ACATGGAGGA ACCTGTGAAG AAGACGACAA TGACTTCACC
    TGTATCTGTC CAGCAGGACG AGGAGGAGCC GTGTGTGAGA AAGTTATAAA GTACTTCATA
    CCATCCTTTG GTGGAAAGTC TTACATGGCG TTCCAGACCA TGAAGGCCTA CCATACTGTA
    CGCATTGCCA TGGAGTTCAG GGCTTCGGAA ATGACTGGGA TATTGCTCTA CAATGGGCAA
    AAAAGCAAAA AGGACTTTCT TTCTCTGACT CTAGTTGAAG GATATGTGGA GCTCAGGTTT
    AACACTGGTT CAGGGACTGC TTCAGTTATC AGTAAAGTCC TGGTAAAGCC GGGTCGCTGG
    CATCAGCTTG TTGTAGTACG TAACAGGCGC AATGCCATGC TGAGCGTGGA CAATGAGCCC
    AGCGTTGAAG GACAAAGTCC TCCAGGAACA GATGGACTCA ACCTGGACAC AGACCTTTTC
    ATCGGCGGAG TGCTTGATGA CATGAGTGCA GACGTAAAGG AGAGGACATC AGTTTCAGCA
    GGTCTGGTGG GCTGCATCCG CATGCTGGAC GTGAACAACC TTGTCTATAA TCTGCAAGAG
    AATGGCGGCA GCGTTCTGTA CGGTTCGGCC ATTGGCGAGT GTGGCAACAA CCCATGCCTG
    CCAAACCCCT GCAAGAACGG AGCGTCCTGT CAGGTCAAAG AAGCCGAGAT TTTCCACTGC
    AAATGTGTCA ACGGTTTCTC TGGCCCGACC TGCGCTGATG CCCACAATCC CTGCGACCCC
    AATAAATGCC ACCCGCCATC ACGGTGCCAG GTCCTTCCAG AGGGAGGTTA CAAATGCGAA
    TGTCCGATGG GACGTGAAGG CAGGCACTGT GAAAAAGTGT CAGACAAGGG AGGCGCTTTC
    ATTCCTTACT TCACTGGAGA TTCTTTTCTG GAGCTCAAAG GCCTCCATAC GTATAACCAG
    GATCTCAGGC AAAAATTCAG CATGACTATC GTTCTACTGG CCAATGACTC CAAAGGCATG
    ATCTTCTACA ATGGTCAGAA GACAGACGGC AAAGGGGATT TCATCTCGCT CTCTCTTAAT
    GACGGCATCC TGGAGTTTAG ATATGACCTG GGCAAAGGGC CAGCTGTCAT TAGGAGTAAA
    GAAAAAATCA AGCTGAACGA GTGGAACACA GTGAATTTGG AACGGGCCAG CAGAAAAGGA
    GAGATTAGCA TTAACGGCAA AGACCCTGTC AGAGGAGAGG CGCCGAAATC ACGCAAGAAT
    CAGCACACGG ATCTCAATCT GAAGGAATCG CTCTTTGTGG GTGGAGCTCC TGATTTCCGC
    AAAGTAGCCA GAGCTGCTGC GATCAAAGAT GGCTTCAAAG GGGCCATTCA GAAGATTACT
    TTGATGGGAA TTCCAATCCT GAAAGCTGAT AATGCCCTTT CGTCCACTGA CGTGTCCATG
    TATCCTAATC ATCCCTGCTC CAAAGACGTT TGTGAGAATG GAGGCCGCTG CAACCCCCAG
    CTGAACAGCT ATGAGTGCGC CTGCCGCCAC GGATTCTCCG GACACCACTG CCAGGACACA
    ATATTTGAGA AGTCCGCTGG GGACACGGAG GCCATTGCGT TTGATGGTCA TACATTCATC
    GAATATCACA ACGGAGTCAC CAAAAGCCCT GAGTCACTGG AGAACCCATC TGACCAGAGC
    GAGAAAGCCC TGCTGGTGAA TAAATTCGAG CTGAGCATCA GGACGGAGGC TACGCATGGT
    CTGATTTTGT GGAGCGGAAA GGGAGTTGAG CGCTCTGACT ACATCGCCCT CGCCATTGTG
    GATGGAAGAG TACAGATGAC CTATGACCTC GGCTCCAAGC CTGTGGTGCT GCGCTCCACT
    GTACGGGTCA ACACCAACCA CTGGATACGA ATTAAGGCCA GCAGAGCGCT CAGAGATGGC
    TCTCTGCAGG TTGGTAATGA GGCAGCTGTC ACTGGGTCTT CACCCTTGGC TGCCACACAG
    CTGGACACAG ACGGGGCTCT CTGGTTGGGT GGACTGGAGG AGCTGTCTGT GGCTCGACGG
    CTGCCCAAAG CTTACAGTAC AGGCTTCATT GGCTGCGTCA AAGACGTGAT AGTGGACGGC
    GTGGAGCTGC ATCTGGTGGA GGACGCCTTA AATACTCCCA AAATATCACA CTGTTCTGCC
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295137.1
    CDS276..6401
    Translation

    Target ORF information:

    RefSeq Version XM_009296862.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296862.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATAAACC TGTTCACTTC
    ACACCGGCAC CTCCTCCTGA TGGTTGCAGA GGGAAGTTGT GCGGATTTGG AGCGGTGTGT
    GAGAGAGACC AAGCTGACCC ATCTAAAGGA GAGTGTGTGT GTAAGAAGAT TGTGTGTACA
    TCTGTGGTGG CGCCAGTGTG CGGATCTGAC TCCTCCACCT ATTCCAATGA GTGTGAGCTG
    GAGAAGGCTC AGTGCAATAC ACAGAGACGC ATCAAGGTGA TGCGCAAAGG CCCCTGCGCC
    CTCAAGGACC CGTGCAGTGA GGTAACCTGT AGCTTTGGCA GCACCTGCAT CCAGTCATCG
    GATGGTCTGT CCGCCAAGTG TATGTGTCCT CTGAGCTGTG AAAACGTCCC CAAGCATGTG
    GTGTGTGGCA GTGACGGCCT GGACTACCAA AGCGAATGTG AACTCAACAT GAAGGCCTGC
    GCCACTCAGA AGAACATCCG TGTGCATCAC CAGGGCCGCT GCGATCCTTG CAGTGACACT
    CTGAACAGCT TAAGCACAGC CTGTCGAGTC AACCCAAAGA CCTACCAGCA GATTACCTTT
    GCCCCGGCTG AGTCTTGTCC GCCTGACGAA ATGCCCATCT GTGCCAGCGA CGGACACACC
    TACGAGAGCA TGTGTCAGAT GGAGCGCACT GCCCTGAAGA ACAACCTGGA GCTCAAAAAG
    GTCTCTTTAG GCAGCTGCAA GGAAAAAGGG GGCTGCCCGA ATGGTTGTAA GTTCAACGCC
    ATATGTTTGC TGGATAATGG AGAGTTTCGG TGCTCCTGCG ATCCCATACA ATGCGACGGC
    ACGTACAAGC CCCTGTGCGG AAAGGATGGC AAGACATACC CCAACGACTG CGAGAGGAAA
    CTGCAGGAAT GCCGGACCCA AAAAGACATC CCTGTCAAAC AACAAGGGCC CTGTGATCTT
    AACCTGGAGA GCCCCTGCCT GAAGAAGACC TGCGAGTTTG GAGCCGTGTG CGTGGTGAAG
    AACAGCGAAG CAGTGTGTGA ATGCTCAGAC GCCTGTCCGC AGGACCAAGA CCCTGTTTGC
    GGCAGCGATG GGCACACGTA CAGCAGCTCA TGTCAAATGA AGGCCATGGG ATGCGCTCTT
    CAGAAACAGA TACAGATGCA ACACAAAGGA CCATGTGCAG ATGAGTCCTG CACCAACTGC
    TCGTTTGGCG CGATCTGTGA TGCCCAGACT GGCCGGTGCG TGTGCCCTAA AGGCTGTTTG
    GAAACACGGC AGCCCGTATG CGGCAGCGAC GGCACCACGT ATGACAACGA GTGCAAGCTC
    AACGTCCAAG CCTGCACTAA ACAGCTGGAT CTCAAGGTGG TGGCCCATGG AGAATGCAAA
    ACCTGTGGCA GCACTGTGTG TAGCTGGGGG TCGCAGTGCG TGAACAACAA GTGTGAGTGT
    CAGCAGTGCA CTGGTCAGCC GGTGAAACCC GTGTGTGGCA GCGACGGCAA CACATACAAC
    AACGACTGCG AGCTGAGACT GGCCTCCTGT CAGAAACAGA GGAAGATAGA GGTGGCCAAG
    CCTGGCGTCT GCGATGAAGA CTGTGGTTCA GGAGGCTCCG GCTCGGGATC TGAGACTTGT
    GAACAGGACC GATGTCGCCG ATATGGAGGA ACGTGGGACG ATGACATGGA GGACGAGCGA
    TGTGTTTGTG AACTAAACTG TCAGATTGTG CTGCACAGCC CGGTGTGCGG CTCTGATGGA
    AACACGTACT CCAGCGACTG TGAGCTCAAG AAGACCAGGT GCGAAAAGCA AATAGACCTG
    AGTGTCCAAA GCCAAGGGCC CTGTCCAACT ATCGTATCAC CACCTCCAGA GCTTGTTGCC
    TCCCAGCACT GTAGCCAAAC AGTTTATGGA TGTTGTGAAG ATAACAAGAC CGCTGCCCTG
    GGTGTTGGAC TGGCTGGATG TCCTAGTATG TGTCAGTGCA ATCATTACGG CTCATACGGA
    GGCACATGTG ACCCCTCGAC TGGCCAGTGT TCCTGTAAAC CTGGTGTCGG TGGACTGAAG
    TGCGACCGCT GTGAGCCCGG ATTTTGGAAC TTCCGTGGCA TTGTCACAGA AAACATGAGC
    GGCTGCACAC CGTGCAACTG TGATGGAGTG GGTTCGGTTC GTGATGACTG CGAGCAGATG
    TCAGGCCTGT GCTCCTGTAA GCCTGGAGTT AAGGGCATGA AATGCAACGT GTGCCCTGAT
    GGCAGCAAGA TGGGAGTCAA CGGCTGTGAC AAAGGACCTA AGGCTCCCAA GTCCTGTGCT
    GAGCTTGTGT GCCACTTTGG GTCTTCATGC GTGGAGGTGA ATGGACAGGC TCACTGTGAG
    TGTCCTTCAC CAGATTGTGA TGAGAAAAAC AAGACCAAGG TATGTGGTTC TGATGGGGTC
    ACCTATGCAG ACCGGTGCCA GCTGAAGACC ATCGCATGCC GACAAGATAA AGAGATCAAG
    GTGGAGCATT TAGGCCAGTG TAAAGAATCC ATTGAGCTGA CGGCCAAACC CACCCCCTTC
    CCAACCACCC ACCGCACCAC CCCTCCCACC GCTCACGTCA AGGTCACCAA ACAGCGACAT
    GGCCACCGCA CCACCACTCC GGACAGCTAT GTGGTGGAGG CAATGCCTCC ACCGAGGTTG
    GATGGAGCGC GAAAGTTTTC CAGCCCTTTC ACCACCCACC GTCCCACTAC ACCCTACAAA
    CCACTCCGCA CCTCATCCCC ACCTCTGGAC TTTGACGAGT CGGGCAGTGG AGAGCCCAGT
    GGGGATGATG AGATGGTGAG CGGAATGGAG GAGAGCGGAG AAGAGCCACT TGGTACAACA
    GTAGCTACTA CCACTCTTCC CACAGCTGAG GAGCGATCGT CCTGTGACAA CACAGCGTTC
    GGCTGCTGTC CCGATGGGAA GACGGCCGCC GTCAACTCAG AGGGCACAAA CTGCCCATCC
    ACCATGCGTT TCAGCGGCTT CCTGCACCTG GATAAGGTTG AGGGTCAGGA GGTCTTCTAC
    ACGCCTGAGA TGGAGGACCC CAAATCGGAA CTGTTTGGTG AAACGGCCCG AAGCATCGAG
    AGCGCTATGA ATGAGTTGTT CCGCAAGTCT GATGTCCAGA AAGACTTCCA GAGTGTCCGT
    GTGAGGAATC TATCCCCCAG CAACTCCATA ATAGCACATT TCGAGGCTCA CTTCGACCCA
    GACACAAGGT TCAATGTGGG GGATATTGAG GGAGCCCTTC TCAAACAGCT TAAGGCGTCT
    AAAGACACTG GCATCGTTGT GAAGAAACCA GAGGAAGAGA ACATTCGCAT CAACAACTAT
    GGTCTTTCAA CTGTTCCTTT CTTTACCACC ACAACAACCA CTGTGGCATC TGTCACGACT
    CCCACTACCA CCACCACCAC AATGTCTCCC AGTTCTGCTT ATACCACTCT CCGGCCCATC
    ACTACTAGAC GACCCTTCAC AAGAACCACC ACGCCAGCAC CAGCCACCAC CACTGCACCA
    CATGTGCCCA AGACCACAGT TTCGCTTATG AACAGACTAG GGCCCCACGC TCACAGTCCA
    TGTGACTCTC AGCCCTGCCG ACATGGAGGA ACCTGTGAAG AAGACGACAA TGACTTCACC
    TGTATCTGTC CAGCAGGACG AGGAGGAGCC GTGTGTGAGA AAGTTATAAA GTACTTCATA
    CCATCCTTTG GTGGAAAGTC TTACATGGCG TTCCAGACCA TGAAGGCCTA CCATACTGTA
    CGCATTGCCA TGGAGTTCAG GGCTTCGGAA ATGACTGGGA TATTGCTCTA CAATGGGCAA
    AAAAGCAAAA AGGACTTTCT TTCTCTGACT CTAGTTGAAG GATATGTGGA GCTCAGGTTT
    AACACTGGTT CAGGGACTGC TTCAGTTATC AGTAAAGTCC TGGTAAAGCC GGGTCGCTGG
    CATCAGCTTG TTGTAGTACG TAACAGGCGC AATGCCATGC TGAGCGTGGA CAATGAGCCC
    AGCGTTGAAG GACAAAGTCC TCCAGGAACA GATGGACTCA ACCTGGACAC AGACCTTTTC
    ATCGGCGGAG TGCTTGATGA CATGAGTGCA GACGTAAAGG AGAGGACATC AGTTTCAGCA
    GGTCTGGTGG GCTGCATCCG CATGCTGGAC GTGAACAACC TTGTCTATAA TCTGCAAGAG
    AATGGCGGCA GCGTTCTGTA CGGTTCGGCC ATTGGCGAGT GTGGCAACAA CCCATGCCTG
    CCAAACCCCT GCAAGAACGG AGCGTCCTGT CAGGTCAAAG AAGCCGAGAT TTTCCACTGC
    AAATGTGTCA ACGGTTTCTC TGGCCCGACC TGCGCTGATG CCCACAATCC CTGCGACCCC
    AATAAATGCC ACCCGCCATC ACGGTGCCAG GTCCTTCCAG AGGGAGGTTA CAAATGCGAA
    TGTCCGATGG GACGTGAAGG CAGGCACTGT GAAAAAGTGT CAGACAAGGG AGGCGCTTTC
    ATTCCTTACT TCACTGGAGA TTCTTTTCTG GAGCTCAAAG GCCTCCATAC GTATAACCAG
    GATCTCAGGC AAAAATTCAG CATGACTATC GTTCTACTGG CCAATGACTC CAAAGGCATG
    ATCTTCTACA ATGGTCAGAA GACAGACGGC AAAGGGGATT TCATCTCGCT CTCTCTTAAT
    GACGGCATCC TGGAGTTTAG ATATGACCTG GGCAAAGGGC CAGCTGTCAT TAGGAGTAAA
    GAAAAAATCA AGCTGAACGA GTGGAACACA GTGAATTTGG AACGGGCCAG CAGAAAAGGA
    GAGATTAGCA TTAACGGCAA AGACCCTGTC AGAGGAGAGG CGCCGAAATC ACGCAAGAAT
    CAGCACACGG ATCTCAATCT GAAGGAATCG CTCTTTGTGG GTGGAGCTCC TGATTTCCGC
    AAAGTAGCCA GAGCTGCTGC GATCAAAGAT GGCTTCAAAG GGGCCATTCA GAAGATTACT
    TTGATGGGAA TTCCAATCCT GAAAGCTGAT AATGCCCTTT CGTCCACTGA CGTGTCCATG
    TATCCTAATC ATCCCTGCTC CAAAGACGTT TGTGAGAATG GAGGCCGCTG CAACCCCCAG
    CTGAACAGCT ATGAGTGCGC CTGCCGCCAC GGATTCTCCG GACACCACTG CCAGGACACA
    ATATTTGAGA AGTCCGCTGG GGACACGGAG GCCATTGCGT TTGATGGTCA TACATTCATC
    GAATATCACA ACGGAGTCAC CAAAAGCCCT GAGTCACTGG AGAACCCATC TGACCAGAGC
    GAGAAAGCCC TGCTGGTGAA TAAATTCGAG CTGAGCATCA GGACGGAGGC TACGCATGGT
    CTGATTTTGT GGAGCGGAAA GGGAGTTGAG CGCTCTGACT ACATCGCCCT CGCCATTGTG
    GATGGAAGAG TACAGATGAC CTATGACCTC GGCTCCAAGC CTGTGGTGCT GCGCTCCACT
    GTACGGGTCA ACACCAACCA CTGGATACGA ATTAAGGCCA GCAGAGCGCT CAGAGATGGC
    TCTCTGCAGG TTGGTAATGA GGCAGCTGTC ACTGGGTCTT CACCCTTGGC TGCCACACAG
    CTGGACACAG ACGGGGCTCT CTGGTTGGGT GGACTGGAGG AGCTGTCTGT GGCTCGACGG
    CTGCCCAAAG CTTACAGTAC AGGCTTCATT GGCTGCGTCA AAGACGTGAT AGTGGACGGC
    GTGGAGCTGC ATCTGGTGGA GGACGCCTTA AATACTCCCA AAATATCACA CTGTTCTGCC
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44577
    Clone ID Related Accession (Same CDS sequence) XM_009296863.2 , XM_009296863.3
    Accession Version XM_009296863.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6114bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product agrin isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296863.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAAAT CACGCAAGAA TCAGCACACG GATCTCAATC TGAAGGAATC GCTCTTTGTG
    GGTGGAGCTC CTGATTTCCG CAAAGTAGCC AGAGCTGCTG CGATCAAAGA TGGCTTCAAA
    GGGGCCATTC AGAAGATTAC TTTGATGGGA ATTCCAATCC TGAAAGCTGA TAATGCCCTT
    TCGTCCACTG ACGTGTCCAT GTATCCTAAT CATCCCTGCT CCAAAGACGT TTGTGAGAAT
    GGAGGCCGCT GCAACCCCCA GCTGAACAGC TATGAGTGCG CCTGCCGCCA CGGATTCTCC
    GGACACCACT GCCAGGACAC AATATTTGAG AAGTCCGCTG GGGACACGGA GGCCATTGCG
    TTTGATGGTC ATACATTCAT CGAATATCAC AACGGAGTCA CCAAAAGCGA GAAAGCCCTG
    CTGGTGAATA AATTCGAGCT GAGCATCAGG ACGGAGGCTA CGCATGGTCT GATTTTGTGG
    AGCGGAAAGG GAGTTGAGCG CTCTGACTAC ATCGCCCTCG CCATTGTGGA TGGAAGAGTA
    CAGATGACCT ATGACCTCGG CTCCAAGCCT GTGGTGCTGC GCTCCACTGT ACGGGTCAAC
    ACCAACCACT GGATACGAAT TAAGGCCAGC AGAGCGCTCA GAGATGGCTC TCTGCAGGTT
    GGTAATGAGG CAGCTGTCAC TGGGTCTTCA CCCTTGGCTG CCACACAGCT GGACACAGAC
    GGGGCTCTCT GGTTGGGTGG ACTGGAGGAG CTGTCTGTGG CTCGACGGCT GCCCAAAGCT
    TACAGTACAG GCTTCATTGG CTGCGTCAAA GACGTGATAG TGGACGGCGT GGAGCTGCAT
    CTGGTGGAGG ACGCCTTAAA TACTCCCAAA ATATCACACT GTTCTGCCAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295138.1
    CDS276..6389
    Translation

    Target ORF information:

    RefSeq Version XM_009296863.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296863.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAAAT CACGCAAGAA TCAGCACACG GATCTCAATC TGAAGGAATC GCTCTTTGTG
    GGTGGAGCTC CTGATTTCCG CAAAGTAGCC AGAGCTGCTG CGATCAAAGA TGGCTTCAAA
    GGGGCCATTC AGAAGATTAC TTTGATGGGA ATTCCAATCC TGAAAGCTGA TAATGCCCTT
    TCGTCCACTG ACGTGTCCAT GTATCCTAAT CATCCCTGCT CCAAAGACGT TTGTGAGAAT
    GGAGGCCGCT GCAACCCCCA GCTGAACAGC TATGAGTGCG CCTGCCGCCA CGGATTCTCC
    GGACACCACT GCCAGGACAC AATATTTGAG AAGTCCGCTG GGGACACGGA GGCCATTGCG
    TTTGATGGTC ATACATTCAT CGAATATCAC AACGGAGTCA CCAAAAGCGA GAAAGCCCTG
    CTGGTGAATA AATTCGAGCT GAGCATCAGG ACGGAGGCTA CGCATGGTCT GATTTTGTGG
    AGCGGAAAGG GAGTTGAGCG CTCTGACTAC ATCGCCCTCG CCATTGTGGA TGGAAGAGTA
    CAGATGACCT ATGACCTCGG CTCCAAGCCT GTGGTGCTGC GCTCCACTGT ACGGGTCAAC
    ACCAACCACT GGATACGAAT TAAGGCCAGC AGAGCGCTCA GAGATGGCTC TCTGCAGGTT
    GGTAATGAGG CAGCTGTCAC TGGGTCTTCA CCCTTGGCTG CCACACAGCT GGACACAGAC
    GGGGCTCTCT GGTTGGGTGG ACTGGAGGAG CTGTCTGTGG CTCGACGGCT GCCCAAAGCT
    TACAGTACAG GCTTCATTGG CTGCGTCAAA GACGTGATAG TGGACGGCGT GGAGCTGCAT
    CTGGTGGAGG ACGCCTTAAA TACTCCCAAA ATATCACACT GTTCTGCCAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44577
    Clone ID Related Accession (Same CDS sequence) XM_009296863.2 , XM_009296863.3
    Accession Version XM_009296863.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6114bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product agrin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296863.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAAAT CACGCAAGAA TCAGCACACG GATCTCAATC TGAAGGAATC GCTCTTTGTG
    GGTGGAGCTC CTGATTTCCG CAAAGTAGCC AGAGCTGCTG CGATCAAAGA TGGCTTCAAA
    GGGGCCATTC AGAAGATTAC TTTGATGGGA ATTCCAATCC TGAAAGCTGA TAATGCCCTT
    TCGTCCACTG ACGTGTCCAT GTATCCTAAT CATCCCTGCT CCAAAGACGT TTGTGAGAAT
    GGAGGCCGCT GCAACCCCCA GCTGAACAGC TATGAGTGCG CCTGCCGCCA CGGATTCTCC
    GGACACCACT GCCAGGACAC AATATTTGAG AAGTCCGCTG GGGACACGGA GGCCATTGCG
    TTTGATGGTC ATACATTCAT CGAATATCAC AACGGAGTCA CCAAAAGCGA GAAAGCCCTG
    CTGGTGAATA AATTCGAGCT GAGCATCAGG ACGGAGGCTA CGCATGGTCT GATTTTGTGG
    AGCGGAAAGG GAGTTGAGCG CTCTGACTAC ATCGCCCTCG CCATTGTGGA TGGAAGAGTA
    CAGATGACCT ATGACCTCGG CTCCAAGCCT GTGGTGCTGC GCTCCACTGT ACGGGTCAAC
    ACCAACCACT GGATACGAAT TAAGGCCAGC AGAGCGCTCA GAGATGGCTC TCTGCAGGTT
    GGTAATGAGG CAGCTGTCAC TGGGTCTTCA CCCTTGGCTG CCACACAGCT GGACACAGAC
    GGGGCTCTCT GGTTGGGTGG ACTGGAGGAG CTGTCTGTGG CTCGACGGCT GCCCAAAGCT
    TACAGTACAG GCTTCATTGG CTGCGTCAAA GACGTGATAG TGGACGGCGT GGAGCTGCAT
    CTGGTGGAGG ACGCCTTAAA TACTCCCAAA ATATCACACT GTTCTGCCAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295138.1
    CDS265..6378
    Translation

    Target ORF information:

    RefSeq Version XM_009296863.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296863.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAAAT CACGCAAGAA TCAGCACACG GATCTCAATC TGAAGGAATC GCTCTTTGTG
    GGTGGAGCTC CTGATTTCCG CAAAGTAGCC AGAGCTGCTG CGATCAAAGA TGGCTTCAAA
    GGGGCCATTC AGAAGATTAC TTTGATGGGA ATTCCAATCC TGAAAGCTGA TAATGCCCTT
    TCGTCCACTG ACGTGTCCAT GTATCCTAAT CATCCCTGCT CCAAAGACGT TTGTGAGAAT
    GGAGGCCGCT GCAACCCCCA GCTGAACAGC TATGAGTGCG CCTGCCGCCA CGGATTCTCC
    GGACACCACT GCCAGGACAC AATATTTGAG AAGTCCGCTG GGGACACGGA GGCCATTGCG
    TTTGATGGTC ATACATTCAT CGAATATCAC AACGGAGTCA CCAAAAGCGA GAAAGCCCTG
    CTGGTGAATA AATTCGAGCT GAGCATCAGG ACGGAGGCTA CGCATGGTCT GATTTTGTGG
    AGCGGAAAGG GAGTTGAGCG CTCTGACTAC ATCGCCCTCG CCATTGTGGA TGGAAGAGTA
    CAGATGACCT ATGACCTCGG CTCCAAGCCT GTGGTGCTGC GCTCCACTGT ACGGGTCAAC
    ACCAACCACT GGATACGAAT TAAGGCCAGC AGAGCGCTCA GAGATGGCTC TCTGCAGGTT
    GGTAATGAGG CAGCTGTCAC TGGGTCTTCA CCCTTGGCTG CCACACAGCT GGACACAGAC
    GGGGCTCTCT GGTTGGGTGG ACTGGAGGAG CTGTCTGTGG CTCGACGGCT GCCCAAAGCT
    TACAGTACAG GCTTCATTGG CTGCGTCAAA GACGTGATAG TGGACGGCGT GGAGCTGCAT
    CTGGTGGAGG ACGCCTTAAA TACTCCCAAA ATATCACACT GTTCTGCCAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44578
    Clone ID Related Accession (Same CDS sequence) XM_009296864.3 , XM_009296864.2
    Accession Version XM_009296864.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6102bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product agrin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296864.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAATC AGCACACGGA TCTCAATCTG AAGGAATCGC TCTTTGTGGG TGGAGCTCCT
    GATTTCCGCA AAGTAGCCAG AGCTGCTGCG ATCAAAGATG GCTTCAAAGG GGCCATTCAG
    AAGATTACTT TGATGGGAAT TCCAATCCTG AAAGCTGATA ATGCCCTTTC GTCCACTGAC
    GTGTCCATGT ATCCTAATCA TCCCTGCTCC AAAGACGTTT GTGAGAATGG AGGCCGCTGC
    AACCCCCAGC TGAACAGCTA TGAGTGCGCC TGCCGCCACG GATTCTCCGG ACACCACTGC
    CAGGACACAA TATTTGAGAA GTCCGCTGGG GACACGGAGG CCATTGCGTT TGATGGTCAT
    ACATTCATCG AATATCACAA CGGAGTCACC AAAAGCGAGA AAGCCCTGCT GGTGAATAAA
    TTCGAGCTGA GCATCAGGAC GGAGGCTACG CATGGTCTGA TTTTGTGGAG CGGAAAGGGA
    GTTGAGCGCT CTGACTACAT CGCCCTCGCC ATTGTGGATG GAAGAGTACA GATGACCTAT
    GACCTCGGCT CCAAGCCTGT GGTGCTGCGC TCCACTGTAC GGGTCAACAC CAACCACTGG
    ATACGAATTA AGGCCAGCAG AGCGCTCAGA GATGGCTCTC TGCAGGTTGG TAATGAGGCA
    GCTGTCACTG GGTCTTCACC CTTGGCTGCC ACACAGCTGG ACACAGACGG GGCTCTCTGG
    TTGGGTGGAC TGGAGGAGCT GTCTGTGGCT CGACGGCTGC CCAAAGCTTA CAGTACAGGC
    TTCATTGGCT GCGTCAAAGA CGTGATAGTG GACGGCGTGG AGCTGCATCT GGTGGAGGAC
    GCCTTAAATA CTCCCAAAAT ATCACACTGT TCTGCCAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295139.1
    CDS265..6366
    Translation

    Target ORF information:

    RefSeq Version XM_009296864.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296864.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAATC AGCACACGGA TCTCAATCTG AAGGAATCGC TCTTTGTGGG TGGAGCTCCT
    GATTTCCGCA AAGTAGCCAG AGCTGCTGCG ATCAAAGATG GCTTCAAAGG GGCCATTCAG
    AAGATTACTT TGATGGGAAT TCCAATCCTG AAAGCTGATA ATGCCCTTTC GTCCACTGAC
    GTGTCCATGT ATCCTAATCA TCCCTGCTCC AAAGACGTTT GTGAGAATGG AGGCCGCTGC
    AACCCCCAGC TGAACAGCTA TGAGTGCGCC TGCCGCCACG GATTCTCCGG ACACCACTGC
    CAGGACACAA TATTTGAGAA GTCCGCTGGG GACACGGAGG CCATTGCGTT TGATGGTCAT
    ACATTCATCG AATATCACAA CGGAGTCACC AAAAGCGAGA AAGCCCTGCT GGTGAATAAA
    TTCGAGCTGA GCATCAGGAC GGAGGCTACG CATGGTCTGA TTTTGTGGAG CGGAAAGGGA
    GTTGAGCGCT CTGACTACAT CGCCCTCGCC ATTGTGGATG GAAGAGTACA GATGACCTAT
    GACCTCGGCT CCAAGCCTGT GGTGCTGCGC TCCACTGTAC GGGTCAACAC CAACCACTGG
    ATACGAATTA AGGCCAGCAG AGCGCTCAGA GATGGCTCTC TGCAGGTTGG TAATGAGGCA
    GCTGTCACTG GGTCTTCACC CTTGGCTGCC ACACAGCTGG ACACAGACGG GGCTCTCTGG
    TTGGGTGGAC TGGAGGAGCT GTCTGTGGCT CGACGGCTGC CCAAAGCTTA CAGTACAGGC
    TTCATTGGCT GCGTCAAAGA CGTGATAGTG GACGGCGTGG AGCTGCATCT GGTGGAGGAC
    GCCTTAAATA CTCCCAAAAT ATCACACTGT TCTGCCAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44578
    Clone ID Related Accession (Same CDS sequence) XM_009296864.3 , XM_009296864.2
    Accession Version XM_009296864.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6102bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product agrin isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296864.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAATC AGCACACGGA TCTCAATCTG AAGGAATCGC TCTTTGTGGG TGGAGCTCCT
    GATTTCCGCA AAGTAGCCAG AGCTGCTGCG ATCAAAGATG GCTTCAAAGG GGCCATTCAG
    AAGATTACTT TGATGGGAAT TCCAATCCTG AAAGCTGATA ATGCCCTTTC GTCCACTGAC
    GTGTCCATGT ATCCTAATCA TCCCTGCTCC AAAGACGTTT GTGAGAATGG AGGCCGCTGC
    AACCCCCAGC TGAACAGCTA TGAGTGCGCC TGCCGCCACG GATTCTCCGG ACACCACTGC
    CAGGACACAA TATTTGAGAA GTCCGCTGGG GACACGGAGG CCATTGCGTT TGATGGTCAT
    ACATTCATCG AATATCACAA CGGAGTCACC AAAAGCGAGA AAGCCCTGCT GGTGAATAAA
    TTCGAGCTGA GCATCAGGAC GGAGGCTACG CATGGTCTGA TTTTGTGGAG CGGAAAGGGA
    GTTGAGCGCT CTGACTACAT CGCCCTCGCC ATTGTGGATG GAAGAGTACA GATGACCTAT
    GACCTCGGCT CCAAGCCTGT GGTGCTGCGC TCCACTGTAC GGGTCAACAC CAACCACTGG
    ATACGAATTA AGGCCAGCAG AGCGCTCAGA GATGGCTCTC TGCAGGTTGG TAATGAGGCA
    GCTGTCACTG GGTCTTCACC CTTGGCTGCC ACACAGCTGG ACACAGACGG GGCTCTCTGG
    TTGGGTGGAC TGGAGGAGCT GTCTGTGGCT CGACGGCTGC CCAAAGCTTA CAGTACAGGC
    TTCATTGGCT GCGTCAAAGA CGTGATAGTG GACGGCGTGG AGCTGCATCT GGTGGAGGAC
    GCCTTAAATA CTCCCAAAAT ATCACACTGT TCTGCCAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295139.1
    CDS276..6377
    Translation

    Target ORF information:

    RefSeq Version XM_009296864.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio agrin (agrn), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009296864.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGGCTCTG CGGACAGAAA AGCGGTTTAT TGGCCCGTTT GGTCATTTGC TCTGGTTTTT 
    GTGCTTTTGA TTCAGCGTTG TCATGGAGGT TGTTCCGAAA AGGAGCTGGA GAAGAGAGAA
    GAAGAAGCGA ATGTAGTTTT AACCGGGACT GTGGAGGAGA TCTTGAACAT GGATCCGGTG
    CACAACACTT ATTCCTGCAA GGTCAGAGTA TGGCGCTACC TGAAAGGCAA GACCATGGTA
    AATGGAGAAG TGCTGCTGGA TGGAGGAAAC AAAGTGATGA TCGGTGGGTT TGGGGACCCC
    CATATTTGTG ACAATCAAGT GGCCACCGGA GATACCCGCA TTTTCTTTGT CAACTTGGCT
    CCTGAGTACA TGTGGCCGAA CCACAAGAAC GAACTGATGC TCAACTCAAG CCTGATGAGG
    ATTACTCTCC GCAACTTGGA GGAGGTAGAG CACTGCGTTG AAGATCATAA AATGATCATT
    GCAGATAAAC CTGTTCACTT CACACCGGCA CCTCCTCCTG ATGGTTGCAG AGGGAAGTTG
    TGCGGATTTG GAGCGGTGTG TGAGAGAGAC CAAGCTGACC CATCTAAAGG AGAGTGTGTG
    TGTAAGAAGA TTGTGTGTAC ATCTGTGGTG GCGCCAGTGT GCGGATCTGA CTCCTCCACC
    TATTCCAATG AGTGTGAGCT GGAGAAGGCT CAGTGCAATA CACAGAGACG CATCAAGGTG
    ATGCGCAAAG GCCCCTGCGC CCTCAAGGAC CCGTGCAGTG AGGTAACCTG TAGCTTTGGC
    AGCACCTGCA TCCAGTCATC GGATGGTCTG TCCGCCAAGT GTATGTGTCC TCTGAGCTGT
    GAAAACGTCC CCAAGCATGT GGTGTGTGGC AGTGACGGCC TGGACTACCA AAGCGAATGT
    GAACTCAACA TGAAGGCCTG CGCCACTCAG AAGAACATCC GTGTGCATCA CCAGGGCCGC
    TGCGATCCTT GCAGTGACAC TCTGAACAGC TTAAGCACAG CCTGTCGAGT CAACCCAAAG
    ACCTACCAGC AGATTACCTT TGCCCCGGCT GAGTCTTGTC CGCCTGACGA AATGCCCATC
    TGTGCCAGCG ACGGACACAC CTACGAGAGC ATGTGTCAGA TGGAGCGCAC TGCCCTGAAG
    AACAACCTGG AGCTCAAAAA GGTCTCTTTA GGCAGCTGCA AGGAAAAAGG GGGCTGCCCG
    AATGGTTGTA AGTTCAACGC CATATGTTTG CTGGATAATG GAGAGTTTCG GTGCTCCTGC
    GATCCCATAC AATGCGACGG CACGTACAAG CCCCTGTGCG GAAAGGATGG CAAGACATAC
    CCCAACGACT GCGAGAGGAA ACTGCAGGAA TGCCGGACCC AAAAAGACAT CCCTGTCAAA
    CAACAAGGGC CCTGTGATCT TAACCTGGAG AGCCCCTGCC TGAAGAAGAC CTGCGAGTTT
    GGAGCCGTGT GCGTGGTGAA GAACAGCGAA GCAGTGTGTG AATGCTCAGA CGCCTGTCCG
    CAGGACCAAG ACCCTGTTTG CGGCAGCGAT GGGCACACGT ACAGCAGCTC ATGTCAAATG
    AAGGCCATGG GATGCGCTCT TCAGAAACAG ATACAGATGC AACACAAAGG ACCATGTGCA
    GATGAGTCCT GCACCAACTG CTCGTTTGGC GCGATCTGTG ATGCCCAGAC TGGCCGGTGC
    GTGTGCCCTA AAGGCTGTTT GGAAACACGG CAGCCCGTAT GCGGCAGCGA CGGCACCACG
    TATGACAACG AGTGCAAGCT CAACGTCCAA GCCTGCACTA AACAGCTGGA TCTCAAGGTG
    GTGGCCCATG GAGAATGCAA AACCTGTGGC AGCACTGTGT GTAGCTGGGG GTCGCAGTGC
    GTGAACAACA AGTGTGAGTG TCAGCAGTGC ACTGGTCAGC CGGTGAAACC CGTGTGTGGC
    AGCGACGGCA ACACATACAA CAACGACTGC GAGCTGAGAC TGGCCTCCTG TCAGAAACAG
    AGGAAGATAG AGGTGGCCAA GCCTGGCGTC TGCGATGAAG ACTGTGGTTC AGGAGGCTCC
    GGCTCGGGAT CTGAGACTTG TGAACAGGAC CGATGTCGCC GATATGGAGG AACGTGGGAC
    GATGACATGG AGGACGAGCG ATGTGTTTGT GAACTAAACT GTCAGATTGT GCTGCACAGC
    CCGGTGTGCG GCTCTGATGG AAACACGTAC TCCAGCGACT GTGAGCTCAA GAAGACCAGG
    TGCGAAAAGC AAATAGACCT GAGTGTCCAA AGCCAAGGGC CCTGTCCAAC TATCGTATCA
    CCACCTCCAG AGCTTGTTGC CTCCCAGCAC TGTAGCCAAA CAGTTTATGG ATGTTGTGAA
    GATAACAAGA CCGCTGCCCT GGGTGTTGGA CTGGCTGGAT GTCCTAGTAT GTGTCAGTGC
    AATCATTACG GCTCATACGG AGGCACATGT GACCCCTCGA CTGGCCAGTG TTCCTGTAAA
    CCTGGTGTCG GTGGACTGAA GTGCGACCGC TGTGAGCCCG GATTTTGGAA CTTCCGTGGC
    ATTGTCACAG AAAACATGAG CGGCTGCACA CCGTGCAACT GTGATGGAGT GGGTTCGGTT
    CGTGATGACT GCGAGCAGAT GTCAGGCCTG TGCTCCTGTA AGCCTGGAGT TAAGGGCATG
    AAATGCAACG TGTGCCCTGA TGGCAGCAAG ATGGGAGTCA ACGGCTGTGA CAAAGGACCT
    AAGGCTCCCA AGTCCTGTGC TGAGCTTGTG TGCCACTTTG GGTCTTCATG CGTGGAGGTG
    AATGGACAGG CTCACTGTGA GTGTCCTTCA CCAGATTGTG ATGAGAAAAA CAAGACCAAG
    GTATGTGGTT CTGATGGGGT CACCTATGCA GACCGGTGCC AGCTGAAGAC CATCGCATGC
    CGACAAGATA AAGAGATCAA GGTGGAGCAT TTAGGCCAGT GTAAAGAATC CATTGAGCTG
    ACGGCCAAAC CCACCCCCTT CCCAACCACC CACCGCACCA CCCCTCCCAC CGCTCACGTC
    AAGGTCACCA AACAGCGACA TGGCCACCGC ACCACCACTC CGGACAGCTA TGTGGTGGAG
    GCAATGCCTC CACCGAGGTT GGATGGAGCG CGAAAGTTTT CCAGCCCTTT CACCACCCAC
    CGTCCCACTA CACCCTACAA ACCACTCCGC ACCTCATCCC CACCTCTGGA CTTTGACGAG
    TCGGGCAGTG GAGAGCCCAG TGGGGATGAT GAGATGGTGA GCGGAATGGA GGAGAGCGGA
    GAAGAGCCAC TTGGTACAAC AGTAGCTACT ACCACTCTTC CCACAGCTGA GGAGCGATCG
    TCCTGTGACA ACACAGCGTT CGGCTGCTGT CCCGATGGGA AGACGGCCGC CGTCAACTCA
    GAGGGCACAA ACTGCCCATC CACCATGCGT TTCAGCGGCT TCCTGCACCT GGATAAGGTT
    GAGGGTCAGG AGGTCTTCTA CACGCCTGAG ATGGAGGACC CCAAATCGGA ACTGTTTGGT
    GAAACGGCCC GAAGCATCGA GAGCGCTATG AATGAGTTGT TCCGCAAGTC TGATGTCCAG
    AAAGACTTCC AGAGTGTCCG TGTGAGGAAT CTATCCCCCA GCAACTCCAT AATAGCACAT
    TTCGAGGCTC ACTTCGACCC AGACACAAGG TTCAATGTGG GGGATATTGA GGGAGCCCTT
    CTCAAACAGC TTAAGGCGTC TAAAGACACT GGCATCGTTG TGAAGAAACC AGAGGAAGAG
    AACATTCGCA TCAACAACTA TGGTCTTTCA ACTGTTCCTT TCTTTACCAC CACAACAACC
    ACTGTGGCAT CTGTCACGAC TCCCACTACC ACCACCACCA CAATGTCTCC CAGTTCTGCT
    TATACCACTC TCCGGCCCAT CACTACTAGA CGACCCTTCA CAAGAACCAC CACGCCAGCA
    CCAGCCACCA CCACTGCACC ACATGTGCCC AAGACCACAG TTTCGCTTAT GAACAGACTA
    GGGCCCCACG CTCACAGTCC ATGTGACTCT CAGCCCTGCC GACATGGAGG AACCTGTGAA
    GAAGACGACA ATGACTTCAC CTGTATCTGT CCAGCAGGAC GAGGAGGAGC CGTGTGTGAG
    AAAGTTATAA AGTACTTCAT ACCATCCTTT GGTGGAAAGT CTTACATGGC GTTCCAGACC
    ATGAAGGCCT ACCATACTGT ACGCATTGCC ATGGAGTTCA GGGCTTCGGA AATGACTGGG
    ATATTGCTCT ACAATGGGCA AAAAAGCAAA AAGGACTTTC TTTCTCTGAC TCTAGTTGAA
    GGATATGTGG AGCTCAGGTT TAACACTGGT TCAGGGACTG CTTCAGTTAT CAGTAAAGTC
    CTGGTAAAGC CGGGTCGCTG GCATCAGCTT GTTGTAGTAC GTAACAGGCG CAATGCCATG
    CTGAGCGTGG ACAATGAGCC CAGCGTTGAA GGACAAAGTC CTCCAGGAAC AGATGGACTC
    AACCTGGACA CAGACCTTTT CATCGGCGGA GTGCTTGATG ACATGAGTGC AGACGTAAAG
    GAGAGGACAT CAGTTTCAGC AGGTCTGGTG GGCTGCATCC GCATGCTGGA CGTGAACAAC
    CTTGTCTATA ATCTGCAAGA GAATGGCGGC AGCGTTCTGT ACGGTTCGGC CATTGGCGAG
    TGTGGCAACA ACCCATGCCT GCCAAACCCC TGCAAGAACG GAGCGTCCTG TCAGGTCAAA
    GAAGCCGAGA TTTTCCACTG CAAATGTGTC AACGGTTTCT CTGGCCCGAC CTGCGCTGAT
    GCCCACAATC CCTGCGACCC CAATAAATGC CACCCGCCAT CACGGTGCCA GGTCCTTCCA
    GAGGGAGGTT ACAAATGCGA ATGTCCGATG GGACGTGAAG GCAGGCACTG TGAAAAAGTG
    TCAGACAAGG GAGGCGCTTT CATTCCTTAC TTCACTGGAG ATTCTTTTCT GGAGCTCAAA
    GGCCTCCATA CGTATAACCA GGATCTCAGG CAAAAATTCA GCATGACTAT CGTTCTACTG
    GCCAATGACT CCAAAGGCAT GATCTTCTAC AATGGTCAGA AGACAGACGG CAAAGGGGAT
    TTCATCTCGC TCTCTCTTAA TGACGGCATC CTGGAGTTTA GATATGACCT GGGCAAAGGG
    CCAGCTGTCA TTAGGAGTAA AGAAAAAATC AAGCTGAACG AGTGGAACAC AGTGAATTTG
    GAACGGGCCA GCAGAAAAGG AGAGATTAGC ATTAACGGCA AAGACCCTGT CAGAGGAGAG
    GCGCCGAATC AGCACACGGA TCTCAATCTG AAGGAATCGC TCTTTGTGGG TGGAGCTCCT
    GATTTCCGCA AAGTAGCCAG AGCTGCTGCG ATCAAAGATG GCTTCAAAGG GGCCATTCAG
    AAGATTACTT TGATGGGAAT TCCAATCCTG AAAGCTGATA ATGCCCTTTC GTCCACTGAC
    GTGTCCATGT ATCCTAATCA TCCCTGCTCC AAAGACGTTT GTGAGAATGG AGGCCGCTGC
    AACCCCCAGC TGAACAGCTA TGAGTGCGCC TGCCGCCACG GATTCTCCGG ACACCACTGC
    CAGGACACAA TATTTGAGAA GTCCGCTGGG GACACGGAGG CCATTGCGTT TGATGGTCAT
    ACATTCATCG AATATCACAA CGGAGTCACC AAAAGCGAGA AAGCCCTGCT GGTGAATAAA
    TTCGAGCTGA GCATCAGGAC GGAGGCTACG CATGGTCTGA TTTTGTGGAG CGGAAAGGGA
    GTTGAGCGCT CTGACTACAT CGCCCTCGCC ATTGTGGATG GAAGAGTACA GATGACCTAT
    GACCTCGGCT CCAAGCCTGT GGTGCTGCGC TCCACTGTAC GGGTCAACAC CAACCACTGG
    ATACGAATTA AGGCCAGCAG AGCGCTCAGA GATGGCTCTC TGCAGGTTGG TAATGAGGCA
    GCTGTCACTG GGTCTTCACC CTTGGCTGCC ACACAGCTGG ACACAGACGG GGCTCTCTGG
    TTGGGTGGAC TGGAGGAGCT GTCTGTGGCT CGACGGCTGC CCAAAGCTTA CAGTACAGGC
    TTCATTGGCT GCGTCAAAGA CGTGATAGTG GACGGCGTGG AGCTGCATCT GGTGGAGGAC
    GCCTTAAATA CTCCCAAAAT ATCACACTGT TCTGCCAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.