agrn gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012967115.2	XP_012822569.1	agrin isoform X7
XM_012967115.3	XP_012822569.1	agrin isoform X7
XM_018096013.1	XP_017951502.1	agrin isoform X2
XM_018096014.1	XP_017951503.1	agrin isoform X3
XM_012967110.2	XP_012822564.1	agrin isoform X4
XM_018096015.1	XP_017951504.1	agrin isoform X5
XM_018096012.2	XP_017951501.1	agrin isoform X1
XM_018096016.2	XP_017951505.1	agrin isoform X6
XM_018096015.2	XP_017951504.1	agrin isoform X5
XM_018096012.1	XP_017951501.1	agrin isoform X1
XM_018096013.2	XP_017951502.1	agrin isoform X2
XM_018096016.1	XP_017951505.1	agrin isoform X6
XM_018096014.2	XP_017951503.1	agrin isoform X3
XM_012967110.3	XP_012822564.1	agrin isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa35299	XM_012967110.3 Latest version!	Xenopus tropicalis agrin (agrn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa35304	XM_012967115.3 Latest version!	Xenopus tropicalis agrin (agrn), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa45875	XM_018096012.2 Latest version!	Xenopus tropicalis agrin (agrn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa45876	XM_018096013.2 Latest version!	Xenopus tropicalis agrin (agrn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa45877	XM_018096014.2 Latest version!	Xenopus tropicalis agrin (agrn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa45878	XM_018096015.2 Latest version!	Xenopus tropicalis agrin (agrn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa45879	XM_018096016.2 Latest version!	Xenopus tropicalis agrin (agrn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OXa35304	XM_012967115.2	Xenopus tropicalis agrin (agrn), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa45878	XM_018096015.1	Xenopus tropicalis agrin (agrn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OXa45875	XM_018096012.1	Xenopus tropicalis agrin (agrn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OXa45876	XM_018096013.1	Xenopus tropicalis agrin (agrn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OXa45879	XM_018096016.1	Xenopus tropicalis agrin (agrn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OXa45877	XM_018096014.1	Xenopus tropicalis agrin (agrn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OXa35299	XM_012967110.2	Xenopus tropicalis agrin (agrn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

agrn ( XM_012967110.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822564.1 Xenopus tropicalis agrin (agrn), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa35299
Clone ID Related Accession (Same CDS sequence)	XM_012967110.2 , XM_012967110.3
Accession Version	XM_012967110.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	agrin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967110.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG ATAAACCTGG CCCTGTCCCT 
AATGCTCAGC CAACTCTACA AGATGTGTGC CGAGGAATGC TCTGCGGATT TGGCGCAGTA 
TGTGAGAAAA GCAGCACCGA CCCATCTCAG GCAACCTGCA TCTGTAAGAA AACCGCCTGC 
GCCTCCATTG TTGCCCCTGT GTGCGGTTCC GACTATTCTA CTTACAGCAA TGAGTGTGAA 
CTGGAGAGAG CCCAGTGCAA CCAGCAGAGG AGAATCAAAG TCATCAGCAA GGGGCCATGC 
GGCACCAAGG ACCCATGCTC AACAGTGTCG TGTAGCTATG GCAGTACCTG TGTCCGCTCA 
ACAGATGGCC AGACGGCAAA GTGTATCTGT CCTGCTACCT GCACAGGGGT TCCAGAAAAT 
GTTGTTTGTG GCAGTGACGG CAAAGATTAT CACAATGAGT GTCAGCTTAA CAAGCAGGCA 
TGCGACAATC AGGAGAATCT CTACAAGAAG TTTGATGGGC GTTGTGATCC ATGCAGAATT 
GCCATGAATA ATCAAAACAA AGCATGTCGA GTAAACCCAC GAACTAGGCA ACTGGAAATG 
CAATACCGAC CAGAATACTG CCCAGTCCGC AGAGATCCTG TTTGCGCAGA TGACGGTGTG 
ACTTATGACA ATGATTGTGT AATGACTCGA TCTGGATTCA TCCGTGGCAT TGAACTGCAG 
AAGGTGCACT CAGGGCAGTG CCAACCGCAA GACAAATGCA AGGAGGACTG TAAACACAAT 
TCTGTGTGTC TTAACCGGAG AGGTGTGACG CGTTGTTCTT GCGACCGTGT TACTTGTGAT 
AGCACTTACA AACCAGTCTG TGGTGGGGAC AGTCGCACGT ATGCCAATGA CTGCGAGAGA 
CAAAAGGCAG AATGCTTTCA GAAAGCTACC ATTCCAGTAA AACACAAAGG CCCATGTGAT 
CTCCATTCCC CCAGTCCATG CCTGAACACA ACCTGCAGTT TTGGAGCAGC ATGTGTAGTA 
AAAAACAAGA AGGCAGTATG CGAATGCCAG CAGGTGTGCC AGGCAGTATA TGACCCAGTG 
TGTGGCAGTG ATAAGCGCAC TTACGGCAAC CCATGTGAAC TGCAGTCAAC AGCCTGCACG 
CTTAAAAAAG AAATAACCAT TTTACACAAG GGGCCTTGTG ACCGCTGTGG GAAATGCCAG 
TTTGGAGCCA TCTGTGAAGC AGAGACTGGC CGCTGTGTAT GTCCAACAGA ATGTGTGCCT 
TCCGCTCAGC CTGTTTGTGG CACTGATGGA AATACATACG AAAGCGAGTG TGAGCTCCAT 
GTACGAGCAT GCACTCAGCA GATTAATCTA CAAGTTGCAG CCCAAGGAGA CTGCAAAACC 
TGTGGCAATA CTCTTTGTAC TTTTGGTGCC AAATGTGTGA ACAACCAGTG TGTGTGCCTA 
ACATGCGAGC GGCAACCCTA CCTACCAGTG TGCGGCAGCA ATGGCGTCAC TTATGATAAC 
CAATGTGAAC TGAAAGCTGC ATCCTGTAAG CAGATGAAGC TGATTGAAGT ATCTCGGGCA 
GGATCTTGTG AAGACGAATG CGGCTCTGGC ACTGACGGTT CTGGTTCTGG TGAGGACAGC 
GAGTGTGAGC AGGATCGATG CAGGCGCTAT GGTGGCTCAT GGGATGAAGA TGCAGAAGAT 
GATCGCTGCG TCTGTGACTT TACCTGTCAT GCTGTGCTTC GAATTTTGGT CTGTGGATCG 
GATGGCATTA CCTACTCTAA TGAGTGTGAG CTAAAGAAAA CTCGGTGTGA GAAACGTCAG 
GATCTGTATG TGGCTGGACA GGGTGCCTGT CGAGCTGCCA CCACCAGCTC TCCTCCTTTG 
GTGCCACACT GCAGTCACAC TGTGTATGGC TGTTGTCCTG ACAATATCAC AGCTGCCCTT 
GGCGTTGGCC TTGCCGGATG CCCAAGCAGC TGTCAGTGCC ACCAGTATGG CTCCTATGGA 
GGAACCTGTG ATCCCACCAC TGGTCAGTGT TCTTGTAAAC CAGGCGTGGG GGGACTGAAA 
TGCGATAGAT GTGAGCCCGG GTTTTGGAAC TTCCGGGGCA TTGTTACGGA TAAGAAAAGC 
GGCTGCACCC CATGTAACTG TGACCCAGTT GGTGCCGTTC GAGATGACTG TGAGCAAATG 
ACAGGACTTT GTTCTTGCAA AACGGGAATC TCAGGTTTGA AGTGCAGCCA GTGCCCTGAT 
GGACGCAAGC CTGGCCTTAC AGGCTGTGAG AAAGATCCTT CAGCTCCTAA ATCTTGTGAT 
GCCATCAAGT GCCAATTTGG TGCAACTTGT GTGGTAATTG GAGGATTTGC TCATTGCGAG 
TGCCCATCTC CCCTGTGTGC TGAGGCGAAT CTGACAAAGG TCTGTGGTTC CGATGATGTG 
ACTTACGGAG ACTGGTGCCA ACTGAAAACC ATTGCTTGCC GTCAGGGCCG CACTATCACA 
GTGAAGCACA CTGGTCCGTG CCAAGAAACT GTCACTCATG CCAACCTTCA GACATTGAGT 
CCAACAGGAA CACCCTCTCC GCCCATAGTT CGGACTCTTC CAACTCCAGG AACCACAAAG 
ATAACAAAGA CCAAGAAAAA GACAGATTCC TTCCAGTTAG TTTTAGAAGC CACATCGCTC 
AGCCCATTTT CCAGCACCAC AATGAGACCT GTGACCCAGA CGCATAGAGC TCACACTTCT 
ACTGTCAGGC CAGCAATCAC TATTCCCACA GTCCATCCCA CTGTGCTTCA GATTTATGAT 
GAATCAGGAA GTGGAAGTGG GGATGAGGAG CTAGAAGCTA GCGGTGATCA AGATTTAAGT 
GGTGTTGAAC CCAAAGAGCC ATTTGATGTG GAGAATGAAC AGAAGGAAGA AGCTGAAGAG 
AAGAGAGATT TTGTAGCCAC CACGGTCATA GAAAGAGCCA GCTGCTATAA CACACCTCTA 
GGATGCTGCT CTGATGGAAA GACCCCAGCT AATGATGCTG AAGGAACAAA TTGCCCAAAA 
ACCAAAATTT TCCAAGGTGT CCTCATTATT GAAGAGTTGG AGGGGCAAGA GGTTTTCTAC 
ACACCAGAAA TGTCAGATTA CAAGTCTGAG CTCTTTGGGG AAACTGCTAG AAGCATTGAG 
AATGCACTGG ATAATCTACT TCGAAATTCA GACATCAAGA GGGATTTCAA GAGCATCCGT 
GTGAGGGACC TTGGACCAAG CAATTCCATC AGAGCCATTG TGGAAGCACA TTTTGATCCA 
GCTACCACCA TGTCTGCTAT GGATGTTCAG AGAGCATTGC TGCGACAGCT CAAGAGCTCC 
AAGAAGAGAG CAATAACCAT AAAGAAGCCT ATTCAGGAAA ATGTCAAGTT CCTGGACTTT 
GGTGAGATCC CTGACTGGGT GCCTCTAATG GTTACAACCA CAACTACAAC GGCAGCAACC 
ACTAGCATGG TAGCTACTAC TGCTGTTGCC CCCACCACCA CAACCCCTGT AGTCGCTACC 
ACAACAACTG TGGCAAGAAC TCCACTACCT AGAAGAATAG TTGCAGTCAA GACCACCCCT 
GTGACAATCA CTCAGCAACC TGTCACCACT CCAAGAATGA CAACAAGACG ACCAGTAACT 
ACAAAAAGAC CAATGACCAC TATTCCTGCC ACCACCAGGA GAAAGCCACC ACGTATTCAA 
GCAGTCACAA AAAAGCCACC CCGGCCATGT GAATCACACC CTTGTCTACA TGGAGGTACT 
TGTGAGGATA ATGGGAAGGA TTTCACCTGT AGCTGCCCAG CTGGCAGAGG AGGAGCTGTG 
TGCGAGAAGA GTATTAAGTA TTTTATTCCT GGGTTTGGTG GTAAGTCATA CCTGGCATTC 
AAGATGATGA AGGCATATCA TACAGTGCGC ATAGCCATGG AGTTCCGATC CTCTGAGCAG 
AACGGGCTTC TCCTATACAA TGGTCAGTCA CTTGGGAAGG ACTTTATCTC ACTTGCAGTT 
AATAATGGCT TTGTGGAACT CAGGTTTAAT ACTGGATCTG GAACTGGAAT AATAACTAGT 
AAAGTGCCCA TTGAACCTGG CAAGTGGCAT CAGTTAGTAG TGAACCGTAA CAGACGCAGT 
GGCATGTTGT CTGTGGATGG TGAATCCCAT GTCAATGGTG AAAGTCCATC TGGAACCGAT 
GGCCTCAACC TAGACACAGA CCTGTTTATA GGAGGAGCTC CTGAGGACCA GATGGCTGTG 
GTATCAGAAA GGACCTCTTT GTCTGTGGGG CTTAAAGGCT GCATCCGACA ACTTGATGTG 
AACAACCAAC TATACAACCT TCAAGAGGGA GGGGCTGATG TCTTGTATGG AAGTGGGGTG 
GGAGAGTGTG GAAACAACCC TTGCCAGCCC AACCCATGCC ATAATGGTGC TGCATGCCAA 
GTAAAGGAGG CTGAAATGTT CCACTGCCAG TGTCTTAGTG GTTATACAGG GCCAACCTGT 
GCAGATGAAC GGAACCCATG TGACCCCAAC CCCTGCCACG TTTCTGCTAC CTGTTTGGTT 
CTACCTGAGG GAGGTGCTAA ATGTGAATGT CCAATGGGCA GAGAGGGAGA GTTTTGTGAG 
AAAGTGAGCA ACCAGGATCT GAATTTGCCA TTCTTACCTC AGTTTAATGG ATACTCTTAC 
TTGGAAATGA AGGGACTACA GACATTTGCC ACTGACCTAC AGGACAAGCT TGCCATGGAA 
GTAGTGTTCC TAGCCAGCAA CCCCAATGGC CTCATCTTCT ATAATGGGCA GAAAACTGAT 
GGAAAAGGAG ATTTTGTGTC ACTGTCCCTT CATGATGGCA ATCTAGAATA CCGTTACGAT 
CTCGGAAAGG GAGCTGCTGT CATCAAGAGT AAGGAAAAGA TTCCACTCAA CACATGGCTC 
AGCGTCTCCC TTGAGCGTAG CGGCAGGAAG GGATTGATGC GGATCAATAA CATGGAACAA 
GTTACTGGGG AATCACCGAA ATCCCGTAAG GCACCACATA CCGCTCTCAA TCTGAAAGAG 
CCTCTATTTG TGGGTGGAGC TCCAGATTTC AGCAAATTAG CCAGGGCAGC GGCTGCCTCT 
ACAAGCTTTG AAGGAGCCAT TCAGAAGATT TCCATTAAGG GGCTGCCTGT TTTGAGAGAA 
GAAAACATCC GCAGTGCCAT TGAGATCTCT ACTTTCCGCA GCCATCCTTG TACCAAGAAA 
CCCTTCCCTT GCCAGAATGG GGGGCTTTGT TCTCCTAATA AAGGCAACTA TGAGTGTGTT 
TGCCAGAGGG GTTTCTCAGG CAGCCAGTGT GAAAAAGTGA CCATTGAGAA AGCAGCTGGA 
GAGTCTGAGG CAGTATCATT TGATGGCAAG ACTTACTTGG AATATCACAT TGCTGTAACC 
AAAAGCCAAC TAACTAACGA GATACCTGAA ACTGGTCCTG TGGACAATCC AGATGACCCC 
AGTGAAAAGG CGCTGCTAGT CAACCAATTT GAGCTGAGCA TCAAGACTGA AGCCACTCAG 
GGTCTCATCC TGTGGAGCGG GAAAGGGACA GAACGTGCCT ATTATATTGC CTTGGCTGTA 
GTTGGAGGTT ATGTTGAGAT GACCTATGAC CTGGGCTCCA AGCCTGTTGT CCTACGATCC 
ACAGTACCTG TGAACACCAA CGAGTGGATT CACATCAAAG CTGTACGAGA CAACCGAGAT 
GGCATTTTAC GAGTTGGCAA TGAGGACCCC GTCCATGGAT CCTCTCCTTT TGGAGTCAAA 
GAGCTGGACA CTGATGGAGC CCTATGGCTG GGTGGAATAG AAAAGCTGAG TGCCTCATAC 
AAGCTGCCCA AAGCGTACAG CACGGGATTC ATTGGCTGCA TGAAGGACGT TCTGGTGGAC 
CAACAAGACC TGCACTTAGT GGAAGATGCA TTGAATAAAC CACCCATATT GTACTGCTCA 
GCCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822564.1
CDS	304..6612
Translation	MGRRWGAPAVLLWTLWALAAVGLGFSRGPCPERELELREEEANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNKVMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEDKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACASIVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDPCSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRVNPRTRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQDKCKEDCKHNSVCLNRRGVTRCSCDRVTCDSTYKPVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCSFGAACVVKNKKAVCECQQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGAICEAETGRCVCPTECVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKCVNNQCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETVTHANLQTLSPTGTPSPPIVRTLPTPGTTKITKTKKKTDSFQLVLEATSLSPFSSTTMRPVTQTHRAHTSTVRPAITIPTVHPTVLQIYDESGSGSGDEELEASGDQDLSGVEPKEPFDVENEQKEEAEEKRDFVATTVIERASCYNTPLGCCSDGKTPANDAEGTNCPKTKIFQGVLIIEELEGQEVFYTPEMSDYKSELFGETARSIENALDNLLRNSDIKRDFKSIRVRDLGPSNSIRAIVEAHFDPATTMSAMDVQRALLRQLKSSKKRAITIKKPIQENVKFLDFGEIPDWVPLMVTTTTTTAATTSMVATTAVAPTTTTPVVATTTTVARTPLPRRIVAVKTTPVTITQQPVTTPRMTTRRPVTTKRPMTTIPATTRRKPPRIQAVTKKPPRPCESHPCLHGGTCEDNGKDFTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNLKEPLFVGGAPDFSKLARAAAASTSFEGAIQKISIKGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLCSPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSQLTNEIPETGPVDNPDDPSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKELDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDALNKPPILYCSAK

Target ORF information:

RefSeq Version	XM_012967110.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis agrin (agrn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967110.3

ORF Insert Sequence:

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG ATAAACCTGG CCCTGTCCCT 
AATGCTCAGC CAACTCTACA AGATGTGTGC CGAGGAATGC TCTGCGGATT TGGCGCAGTA 
TGTGAGAAAA GCAGCACCGA CCCATCTCAG GCAACCTGCA TCTGTAAGAA AACCGCCTGC 
GCCTCCATTG TTGCCCCTGT GTGCGGTTCC GACTATTCTA CTTACAGCAA TGAGTGTGAA 
CTGGAGAGAG CCCAGTGCAA CCAGCAGAGG AGAATCAAAG TCATCAGCAA GGGGCCATGC 
GGCACCAAGG ACCCATGCTC AACAGTGTCG TGTAGCTATG GCAGTACCTG TGTCCGCTCA 
ACAGATGGCC AGACGGCAAA GTGTATCTGT CCTGCTACCT GCACAGGGGT TCCAGAAAAT 
GTTGTTTGTG GCAGTGACGG CAAAGATTAT CACAATGAGT GTCAGCTTAA CAAGCAGGCA 
TGCGACAATC AGGAGAATCT CTACAAGAAG TTTGATGGGC GTTGTGATCC ATGCAGAATT 
GCCATGAATA ATCAAAACAA AGCATGTCGA GTAAACCCAC GAACTAGGCA ACTGGAAATG 
CAATACCGAC CAGAATACTG CCCAGTCCGC AGAGATCCTG TTTGCGCAGA TGACGGTGTG 
ACTTATGACA ATGATTGTGT AATGACTCGA TCTGGATTCA TCCGTGGCAT TGAACTGCAG 
AAGGTGCACT CAGGGCAGTG CCAACCGCAA GACAAATGCA AGGAGGACTG TAAACACAAT 
TCTGTGTGTC TTAACCGGAG AGGTGTGACG CGTTGTTCTT GCGACCGTGT TACTTGTGAT 
AGCACTTACA AACCAGTCTG TGGTGGGGAC AGTCGCACGT ATGCCAATGA CTGCGAGAGA 
CAAAAGGCAG AATGCTTTCA GAAAGCTACC ATTCCAGTAA AACACAAAGG CCCATGTGAT 
CTCCATTCCC CCAGTCCATG CCTGAACACA ACCTGCAGTT TTGGAGCAGC ATGTGTAGTA 
AAAAACAAGA AGGCAGTATG CGAATGCCAG CAGGTGTGCC AGGCAGTATA TGACCCAGTG 
TGTGGCAGTG ATAAGCGCAC TTACGGCAAC CCATGTGAAC TGCAGTCAAC AGCCTGCACG 
CTTAAAAAAG AAATAACCAT TTTACACAAG GGGCCTTGTG ACCGCTGTGG GAAATGCCAG 
TTTGGAGCCA TCTGTGAAGC AGAGACTGGC CGCTGTGTAT GTCCAACAGA ATGTGTGCCT 
TCCGCTCAGC CTGTTTGTGG CACTGATGGA AATACATACG AAAGCGAGTG TGAGCTCCAT 
GTACGAGCAT GCACTCAGCA GATTAATCTA CAAGTTGCAG CCCAAGGAGA CTGCAAAACC 
TGTGGCAATA CTCTTTGTAC TTTTGGTGCC AAATGTGTGA ACAACCAGTG TGTGTGCCTA 
ACATGCGAGC GGCAACCCTA CCTACCAGTG TGCGGCAGCA ATGGCGTCAC TTATGATAAC 
CAATGTGAAC TGAAAGCTGC ATCCTGTAAG CAGATGAAGC TGATTGAAGT ATCTCGGGCA 
GGATCTTGTG AAGACGAATG CGGCTCTGGC ACTGACGGTT CTGGTTCTGG TGAGGACAGC 
GAGTGTGAGC AGGATCGATG CAGGCGCTAT GGTGGCTCAT GGGATGAAGA TGCAGAAGAT 
GATCGCTGCG TCTGTGACTT TACCTGTCAT GCTGTGCTTC GAATTTTGGT CTGTGGATCG 
GATGGCATTA CCTACTCTAA TGAGTGTGAG CTAAAGAAAA CTCGGTGTGA GAAACGTCAG 
GATCTGTATG TGGCTGGACA GGGTGCCTGT CGAGCTGCCA CCACCAGCTC TCCTCCTTTG 
GTGCCACACT GCAGTCACAC TGTGTATGGC TGTTGTCCTG ACAATATCAC AGCTGCCCTT 
GGCGTTGGCC TTGCCGGATG CCCAAGCAGC TGTCAGTGCC ACCAGTATGG CTCCTATGGA 
GGAACCTGTG ATCCCACCAC TGGTCAGTGT TCTTGTAAAC CAGGCGTGGG GGGACTGAAA 
TGCGATAGAT GTGAGCCCGG GTTTTGGAAC TTCCGGGGCA TTGTTACGGA TAAGAAAAGC 
GGCTGCACCC CATGTAACTG TGACCCAGTT GGTGCCGTTC GAGATGACTG TGAGCAAATG 
ACAGGACTTT GTTCTTGCAA AACGGGAATC TCAGGTTTGA AGTGCAGCCA GTGCCCTGAT 
GGACGCAAGC CTGGCCTTAC AGGCTGTGAG AAAGATCCTT CAGCTCCTAA ATCTTGTGAT 
GCCATCAAGT GCCAATTTGG TGCAACTTGT GTGGTAATTG GAGGATTTGC TCATTGCGAG 
TGCCCATCTC CCCTGTGTGC TGAGGCGAAT CTGACAAAGG TCTGTGGTTC CGATGATGTG 
ACTTACGGAG ACTGGTGCCA ACTGAAAACC ATTGCTTGCC GTCAGGGCCG CACTATCACA 
GTGAAGCACA CTGGTCCGTG CCAAGAAACT GTCACTCATG CCAACCTTCA GACATTGAGT 
CCAACAGGAA CACCCTCTCC GCCCATAGTT CGGACTCTTC CAACTCCAGG AACCACAAAG 
ATAACAAAGA CCAAGAAAAA GACAGATTCC TTCCAGTTAG TTTTAGAAGC CACATCGCTC 
AGCCCATTTT CCAGCACCAC AATGAGACCT GTGACCCAGA CGCATAGAGC TCACACTTCT 
ACTGTCAGGC CAGCAATCAC TATTCCCACA GTCCATCCCA CTGTGCTTCA GATTTATGAT 
GAATCAGGAA GTGGAAGTGG GGATGAGGAG CTAGAAGCTA GCGGTGATCA AGATTTAAGT 
GGTGTTGAAC CCAAAGAGCC ATTTGATGTG GAGAATGAAC AGAAGGAAGA AGCTGAAGAG 
AAGAGAGATT TTGTAGCCAC CACGGTCATA GAAAGAGCCA GCTGCTATAA CACACCTCTA 
GGATGCTGCT CTGATGGAAA GACCCCAGCT AATGATGCTG AAGGAACAAA TTGCCCAAAA 
ACCAAAATTT TCCAAGGTGT CCTCATTATT GAAGAGTTGG AGGGGCAAGA GGTTTTCTAC 
ACACCAGAAA TGTCAGATTA CAAGTCTGAG CTCTTTGGGG AAACTGCTAG AAGCATTGAG 
AATGCACTGG ATAATCTACT TCGAAATTCA GACATCAAGA GGGATTTCAA GAGCATCCGT 
GTGAGGGACC TTGGACCAAG CAATTCCATC AGAGCCATTG TGGAAGCACA TTTTGATCCA 
GCTACCACCA TGTCTGCTAT GGATGTTCAG AGAGCATTGC TGCGACAGCT CAAGAGCTCC 
AAGAAGAGAG CAATAACCAT AAAGAAGCCT ATTCAGGAAA ATGTCAAGTT CCTGGACTTT 
GGTGAGATCC CTGACTGGGT GCCTCTAATG GTTACAACCA CAACTACAAC GGCAGCAACC 
ACTAGCATGG TAGCTACTAC TGCTGTTGCC CCCACCACCA CAACCCCTGT AGTCGCTACC 
ACAACAACTG TGGCAAGAAC TCCACTACCT AGAAGAATAG TTGCAGTCAA GACCACCCCT 
GTGACAATCA CTCAGCAACC TGTCACCACT CCAAGAATGA CAACAAGACG ACCAGTAACT 
ACAAAAAGAC CAATGACCAC TATTCCTGCC ACCACCAGGA GAAAGCCACC ACGTATTCAA 
GCAGTCACAA AAAAGCCACC CCGGCCATGT GAATCACACC CTTGTCTACA TGGAGGTACT 
TGTGAGGATA ATGGGAAGGA TTTCACCTGT AGCTGCCCAG CTGGCAGAGG AGGAGCTGTG 
TGCGAGAAGA GTATTAAGTA TTTTATTCCT GGGTTTGGTG GTAAGTCATA CCTGGCATTC 
AAGATGATGA AGGCATATCA TACAGTGCGC ATAGCCATGG AGTTCCGATC CTCTGAGCAG 
AACGGGCTTC TCCTATACAA TGGTCAGTCA CTTGGGAAGG ACTTTATCTC ACTTGCAGTT 
AATAATGGCT TTGTGGAACT CAGGTTTAAT ACTGGATCTG GAACTGGAAT AATAACTAGT 
AAAGTGCCCA TTGAACCTGG CAAGTGGCAT CAGTTAGTAG TGAACCGTAA CAGACGCAGT 
GGCATGTTGT CTGTGGATGG TGAATCCCAT GTCAATGGTG AAAGTCCATC TGGAACCGAT 
GGCCTCAACC TAGACACAGA CCTGTTTATA GGAGGAGCTC CTGAGGACCA GATGGCTGTG 
GTATCAGAAA GGACCTCTTT GTCTGTGGGG CTTAAAGGCT GCATCCGACA ACTTGATGTG 
AACAACCAAC TATACAACCT TCAAGAGGGA GGGGCTGATG TCTTGTATGG AAGTGGGGTG 
GGAGAGTGTG GAAACAACCC TTGCCAGCCC AACCCATGCC ATAATGGTGC TGCATGCCAA 
GTAAAGGAGG CTGAAATGTT CCACTGCCAG TGTCTTAGTG GTTATACAGG GCCAACCTGT 
GCAGATGAAC GGAACCCATG TGACCCCAAC CCCTGCCACG TTTCTGCTAC CTGTTTGGTT 
CTACCTGAGG GAGGTGCTAA ATGTGAATGT CCAATGGGCA GAGAGGGAGA GTTTTGTGAG 
AAAGTGAGCA ACCAGGATCT GAATTTGCCA TTCTTACCTC AGTTTAATGG ATACTCTTAC 
TTGGAAATGA AGGGACTACA GACATTTGCC ACTGACCTAC AGGACAAGCT TGCCATGGAA 
GTAGTGTTCC TAGCCAGCAA CCCCAATGGC CTCATCTTCT ATAATGGGCA GAAAACTGAT 
GGAAAAGGAG ATTTTGTGTC ACTGTCCCTT CATGATGGCA ATCTAGAATA CCGTTACGAT 
CTCGGAAAGG GAGCTGCTGT CATCAAGAGT AAGGAAAAGA TTCCACTCAA CACATGGCTC 
AGCGTCTCCC TTGAGCGTAG CGGCAGGAAG GGATTGATGC GGATCAATAA CATGGAACAA 
GTTACTGGGG AATCACCGAA ATCCCGTAAG GCACCACATA CCGCTCTCAA TCTGAAAGAG 
CCTCTATTTG TGGGTGGAGC TCCAGATTTC AGCAAATTAG CCAGGGCAGC GGCTGCCTCT 
ACAAGCTTTG AAGGAGCCAT TCAGAAGATT TCCATTAAGG GGCTGCCTGT TTTGAGAGAA 
GAAAACATCC GCAGTGCCAT TGAGATCTCT ACTTTCCGCA GCCATCCTTG TACCAAGAAA 
CCCTTCCCTT GCCAGAATGG GGGGCTTTGT TCTCCTAATA AAGGCAACTA TGAGTGTGTT 
TGCCAGAGGG GTTTCTCAGG CAGCCAGTGT GAAAAAGTGA CCATTGAGAA AGCAGCTGGA 
GAGTCTGAGG CAGTATCATT TGATGGCAAG ACTTACTTGG AATATCACAT TGCTGTAACC 
AAAAGCCAAC TAACTAACGA GATACCTGAA ACTGGTCCTG TGGACAATCC AGATGACCCC 
AGTGAAAAGG CGCTGCTAGT CAACCAATTT GAGCTGAGCA TCAAGACTGA AGCCACTCAG 
GGTCTCATCC TGTGGAGCGG GAAAGGGACA GAACGTGCCT ATTATATTGC CTTGGCTGTA 
GTTGGAGGTT ATGTTGAGAT GACCTATGAC CTGGGCTCCA AGCCTGTTGT CCTACGATCC 
ACAGTACCTG TGAACACCAA CGAGTGGATT CACATCAAAG CTGTACGAGA CAACCGAGAT 
GGCATTTTAC GAGTTGGCAA TGAGGACCCC GTCCATGGAT CCTCTCCTTT TGGAGTCAAA 
GAGCTGGACA CTGATGGAGC CCTATGGCTG GGTGGAATAG AAAAGCTGAG TGCCTCATAC 
AAGCTGCCCA AAGCGTACAG CACGGGATTC ATTGGCTGCA TGAAGGACGT TCTGGTGGAC 
CAACAAGACC TGCACTTAGT GGAAGATGCA TTGAATAAAC CACCCATATT GTACTGCTCA 
GCCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agrn ( XM_012967115.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822569.1 Xenopus tropicalis agrin (agrn), transcript variant X7, mRNA.

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CloneID	OXa35304
Clone ID Related Accession (Same CDS sequence)	XM_012967115.2 , XM_012967115.3
Accession Version	XM_012967115.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	agrin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967115.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGCT GCCACTACCC TGCTCCAGGT TTGGAGAGGG ACCGGATCTA TCAGCATAAA 
GTCTCACTGG TTGTGCGCTA CTTCATGATC CCTTGTAACA TATGCCTGAT CCTGCTTGCC 
ACTTCCACGC TTGGATTTGC CGTCCTGCTG TTCCTGAATA ACTATAAACC TGGCCCTGTC 
CCTAATGCTC AGCCAACTCT ACAAGATGTG TGCCGAGGAA TGCTCTGCGG ATTTGGCGCA 
GTATGTGAGA AAAGCAGCAC CGACCCATCT CAGGCAACCT GCATCTGTAA GAAAACCGCC 
TGCGCCTCCA TTGTTGCCCC TGTGTGCGGT TCCGACTATT CTACTTACAG CAATGAGTGT 
GAACTGGAGA GAGCCCAGTG CAACCAGCAG AGGAGAATCA AAGTCATCAG CAAGGGGCCA 
TGCGGCACCA AGGACCCATG CTCAACAGTG TCGTGTAGCT ATGGCAGTAC CTGTGTCCGC 
TCAACAGATG GCCAGACGGC AAAGTGTATC TGTCCTGCTA CCTGCACAGG GGTTCCAGAA 
AATGTTGTTT GTGGCAGTGA CGGCAAAGAT TATCACAATG AGTGTCAGCT TAACAAGCAG 
GCATGCGACA ATCAGGAGAA TCTCTACAAG AAGTTTGATG GGCGTTGTGA TCCATGCAGA 
ATTGCCATGA ATAATCAAAA CAAAGCATGT CGAGTAAACC CACGAACTAG GCAACTGGAA 
ATGCAATACC GACCAGAATA CTGCCCAGTC CGCAGAGATC CTGTTTGCGC AGATGACGGT 
GTGACTTATG ACAATGATTG TGTAATGACT CGATCTGGAT TCATCCGTGG CATTGAACTG 
CAGAAGGTGC ACTCAGGGCA GTGCCAACCG CAAGACAAAT GCAAGGAGGA CTGTAAACAC 
AATTCTGTGT GTCTTAACCG GAGAGGTGTG ACGCGTTGTT CTTGCGACCG TGTTACTTGT 
GATAGCACTT ACAAACCAGT CTGTGGTGGG GACAGTCGCA CGTATGCCAA TGACTGCGAG 
AGACAAAAGG CAGAATGCTT TCAGAAAGCT ACCATTCCAG TAAAACACAA AGGCCCATGT 
GATCTCCATT CCCCCAGTCC ATGCCTGAAC ACAACCTGCA GTTTTGGAGC AGCATGTGTA 
GTAAAAAACA AGAAGGCAGT ATGCGAATGC CAGCAGGTGT GCCAGGCAGT ATATGACCCA 
GTGTGTGGCA GTGATAAGCG CACTTACGGC AACCCATGTG AACTGCAGTC AACAGCCTGC 
ACGCTTAAAA AAGAAATAAC CATTTTACAC AAGGGGCCTT GTGACCGCTG TGGGAAATGC 
CAGTTTGGAG CCATCTGTGA AGCAGAGACT GGCCGCTGTG TATGTCCAAC AGAATGTGTG 
CCTTCCGCTC AGCCTGTTTG TGGCACTGAT GGAAATACAT ACGAAAGCGA GTGTGAGCTC 
CATGTACGAG CATGCACTCA GCAGATTAAT CTACAAGTTG CAGCCCAAGG AGACTGCAAA 
ACCTGTGGCA ATACTCTTTG TACTTTTGGT GCCAAATGTG TGAACAACCA GTGTGTGTGC 
CTAACATGCG AGCGGCAACC CTACCTACCA GTGTGCGGCA GCAATGGCGT CACTTATGAT 
AACCAATGTG AACTGAAAGC TGCATCCTGT AAGCAGATGA AGCTGATTGA AGTATCTCGG 
GCAGGATCTT GTGAAGACGA ATGCGGCTCT GGCACTGACG GTTCTGGTTC TGGTGAGGAC 
AGCGAGTGTG AGCAGGATCG ATGCAGGCGC TATGGTGGCT CATGGGATGA AGATGCAGAA 
GATGATCGCT GCGTCTGTGA CTTTACCTGT CATGCTGTGC TTCGAATTTT GGTCTGTGGA 
TCGGATGGCA TTACCTACTC TAATGAGTGT GAGCTAAAGA AAACTCGGTG TGAGAAACGT 
CAGGATCTGT ATGTGGCTGG ACAGGGTGCC TGTCGAGCTG CCACCACCAG CTCTCCTCCT 
TTGGTGCCAC ACTGCAGTCA CACTGTGTAT GGCTGTTGTC CTGACAATAT CACAGCTGCC 
CTTGGCGTTG GCCTTGCCGG ATGCCCAAGC AGCTGTCAGT GCCACCAGTA TGGCTCCTAT 
GGAGGAACCT GTGATCCCAC CACTGGTCAG TGTTCTTGTA AACCAGGCGT GGGGGGACTG 
AAATGCGATA GATGTGAGCC CGGGTTTTGG AACTTCCGGG GCATTGTTAC GGATAAGAAA 
AGCGGCTGCA CCCCATGTAA CTGTGACCCA GTTGGTGCCG TTCGAGATGA CTGTGAGCAA 
ATGACAGGAC TTTGTTCTTG CAAAACGGGA ATCTCAGGTT TGAAGTGCAG CCAGTGCCCT 
GATGGACGCA AGCCTGGCCT TACAGGCTGT GAGAAAGATC CTTCAGCTCC TAAATCTTGT 
GATGCCATCA AGTGCCAATT TGGTGCAACT TGTGTGGTAA TTGGAGGATT TGCTCATTGC 
GAGTGCCCAT CTCCCCTGTG TGCTGAGGCG AATCTGACAA AGGTCTGTGG TTCCGATGAT 
GTGACTTACG GAGACTGGTG CCAACTGAAA ACCATTGCTT GCCGTCAGGG CCGCACTATC 
ACAGTGAAGC ACACTGGTCC GTGCCAAGAA ACTGTCACTC ATGCCAACCT TCAGACATTG 
AGTCCAACAG GAACACCCTC TCCGCCCATA GTTCGGACTC TTCCAACTCC AGGAACCACA 
AAGATAACAA AGACCAAGAA AAAGACAGAT TCCTTCCAGT TAGTTTTAGA AGCCACATCG 
CTCAGCCCAT TTTCCAGCAC CACAATGAGA CCTGTGACCC AGACGCATAG AGCTCACACT 
TCTACTGTCA GGCCAGCAAT CACTATTCCC ACAGTCCATC CCACTGTGCT TCAGATTTAT 
GATGAATCAG GAAGTGGAAG TGGGGATGAG GAGCTAGAAG CTAGCGGTGA TCAAGATTTA 
AGTGGTGTTG AACCCAAAGA GCCATTTGAT GTGGAGAATG AACAGAAGGA AGAAGCTGAA 
GAGAAGAGAG ATTTTGTAGC CACCACGGTC ATAGAAAGAG CCAGCTGCTA TAACACACCT 
CTAGGATGCT GCTCTGATGG AAAGACCCCA GCTAATGATG CTGAAGGAAC AAATTGCCCA 
AAAACCAAAA TTTTCCAAGG TGTCCTCATT ATTGAAGAGT TGGAGGGGCA AGAGGTTTTC 
TACACACCAG AAATGTCAGA TTACAAGTCT GAGCTCTTTG GGGAAACTGC TAGAAGCATT 
GAGAATGCAC TGGATAATCT ACTTCGAAAT TCAGACATCA AGAGGGATTT CAAGAGCATC 
CGTGTGAGGG ACCTTGGACC AAGCAATTCC ATCAGAGCCA TTGTGGAAGC ACATTTTGAT 
CCAGCTACCA CCATGTCTGC TATGGATGTT CAGAGAGCAT TGCTGCGACA GCTCAAGAGC 
TCCAAGAAGA GAGCAATAAC CATAAAGAAG CCTATTCAGG AAAATGTCAA GTTCCTGGAC 
TTTGGTGAGA TCCCTGACTG GGTGCCTCTA ATGGTTACAA CCACAACTAC AACGGCAGCA 
ACCACTAGCA TGGTAGCTAC TACTGCTGTT GCCCCCACCA CCACAACCCC TGTAGTCGCT 
ACCACAACAA CTGTGGCAAG AACTCCACTA CCTAGAAGAA TAGTTGCAGT CAAGACCACC 
CCTGTGACAA TCACTCAGCA ACCTGTCACC ACTCCAAGAA TGACAACAAG ACGACCAGTA 
ACTACAAAAA GACCAATGAC CACTATTCCT GCCACCACCA GGAGAAAGCC ACCACGTATT 
CAAGCAGTCA CAAAAAAGCC ACCCCGGCCA TGTGAATCAC ACCCTTGTCT ACATGGAGGT 
ACTTGTGAGG ATAATGGGAA GGATTTCACC TGTAGCTGCC CAGCTGGCAG AGGAGGAGCT 
GTGTGCGAGA AGAGTATTAA GTATTTTATT CCTGGGTTTG GTGGTAAGTC ATACCTGGCA 
TTCAAGATGA TGAAGGCATA TCATACAGTG CGCATAGCCA TGGAGTTCCG ATCCTCTGAG 
CAGAACGGGC TTCTCCTATA CAATGGTCAG TCACTTGGGA AGGACTTTAT CTCACTTGCA 
GTTAATAATG GCTTTGTGGA ACTCAGGTTT AATACTGGAT CTGGAACTGG AATAATAACT 
AGTAAAGTGC CCATTGAACC TGGCAAGTGG CATCAGTTAG TAGTGAACCG TAACAGACGC 
AGTGGCATGT TGTCTGTGGA TGGTGAATCC CATGTCAATG GTGAAAGTCC ATCTGGAACC 
GATGGCCTCA ACCTAGACAC AGACCTGTTT ATAGGAGGAG CTCCTGAGGA CCAGATGGCT 
GTGGTATCAG AAAGGACCTC TTTGTCTGTG GGGCTTAAAG GCTGCATCCG ACAACTTGAT 
GTGAACAACC AACTATACAA CCTTCAAGAG GGAGGGGCTG ATGTCTTGTA TGGAAGTGGG 
GTGGGAGAGT GTGGAAACAA CCCTTGCCAG CCCAACCCAT GCCATAATGG TGCTGCATGC 
CAAGTAAAGG AGGCTGAAAT GTTCCACTGC CAGTGTCTTA GTGGTTATAC AGGGCCAACC 
TGTGCAGATG AACGGAACCC ATGTGACCCC AACCCCTGCC ACGTTTCTGC TACCTGTTTG 
GTTCTACCTG AGGGAGGTGC TAAATGTGAA TGTCCAATGG GCAGAGAGGG AGAGTTTTGT 
GAGAAAGTGA GCAACCAGGA TCTGAATTTG CCATTCTTAC CTCAGTTTAA TGGATACTCT 
TACTTGGAAA TGAAGGGACT ACAGACATTT GCCACTGACC TACAGGACAA GCTTGCCATG 
GAAGTAGTGT TCCTAGCCAG CAACCCCAAT GGCCTCATCT TCTATAATGG GCAGAAAACT 
GATGGAAAAG GAGATTTTGT GTCACTGTCC CTTCATGATG GCAATCTAGA ATACCGTTAC 
GATCTCGGAA AGGGAGCTGC TGTCATCAAG AGTAAGGAAA AGATTCCACT CAACACATGG 
CTCAGCGTCT CCCTTGAGCG TAGCGGCAGG AAGGGATTGA TGCGGATCAA TAACATGGAA 
CAAGTTACTG GGGAATCACC GAAATCCCGT AAGGCACCAC ATACCGCTCT CAATCTGAAA 
GAGCCTCTAT TTGTGGGTGG AGCTCCAGAT TTCAGCAAAT TAGCCAGGGC AGCGGCTGCC 
TCTACAAGCT TTGAAGGAGC CATTCAGAAG ATTTCCATTA AGGGGCTGCC TGTTTTGAGA 
GAAGAAAACA TCCGCAGTGC CATTGAGATC TCTACTTTCC GCAGCCATCC TTGTACCAAG 
AAACCCTTCC CTTGCCAGAA TGGGGGGCTT TGTTCTCCTA ATAAAGGCAA CTATGAGTGT 
GTTTGCCAGA GGGGTTTCTC AGGCAGCCAG TGTGAAAAAG TGACCATTGA GAAAGCAGCT 
GGAGAGTCTG AGGCAGTATC ATTTGATGGC AAGACTTACT TGGAATATCA CATTGCTGTA 
ACCAAAAGCC AACTAACTAA CGAGATACCT GAAACTGGTC CTGTGGACAA TCCAGATGAC 
CCCAGTGAAA AGGCGCTGCT AGTCAACCAA TTTGAGCTGA GCATCAAGAC TGAAGCCACT 
CAGGGTCTCA TCCTGTGGAG CGGGAAAGGG ACAGAACGTG CCTATTATAT TGCCTTGGCT 
GTAGTTGGAG GTTATGTTGA GATGACCTAT GACCTGGGCT CCAAGCCTGT TGTCCTACGA 
TCCACAGTAC CTGTGAACAC CAACGAGTGG ATTCACATCA AAGCTGTACG AGACAACCGA 
GATGGCATTT TACGAGTTGG CAATGAGGAC CCCGTCCATG GATCCTCTCC TTTTGGAGTC 
AAAGAGCTGG ACACTGATGG AGCCCTATGG CTGGGTGGAA TAGAAAAGCT GAGTGCCTCA 
TACAAGCTGC CCAAAGCGTA CAGCACGGGA TTCATTGGCT GCATGAAGGA CGTTCTGGTG 
GACCAACAAG ACCTGCACTT AGTGGAAGAT GCATTGAATA AACCACCCAT ATTGTACTGC 
TCAGCCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822569.1
CDS	1708..7719
Translation	MTGCHYPAPGLERDRIYQHKVSLVVRYFMIPCNICLILLATSTLGFAVLLFLNNYKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACASIVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDPCSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRVNPRTRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQDKCKEDCKHNSVCLNRRGVTRCSCDRVTCDSTYKPVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCSFGAACVVKNKKAVCECQQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGAICEAETGRCVCPTECVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKCVNNQCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETVTHANLQTLSPTGTPSPPIVRTLPTPGTTKITKTKKKTDSFQLVLEATSLSPFSSTTMRPVTQTHRAHTSTVRPAITIPTVHPTVLQIYDESGSGSGDEELEASGDQDLSGVEPKEPFDVENEQKEEAEEKRDFVATTVIERASCYNTPLGCCSDGKTPANDAEGTNCPKTKIFQGVLIIEELEGQEVFYTPEMSDYKSELFGETARSIENALDNLLRNSDIKRDFKSIRVRDLGPSNSIRAIVEAHFDPATTMSAMDVQRALLRQLKSSKKRAITIKKPIQENVKFLDFGEIPDWVPLMVTTTTTTAATTSMVATTAVAPTTTTPVVATTTTVARTPLPRRIVAVKTTPVTITQQPVTTPRMTTRRPVTTKRPMTTIPATTRRKPPRIQAVTKKPPRPCESHPCLHGGTCEDNGKDFTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNLKEPLFVGGAPDFSKLARAAAASTSFEGAIQKISIKGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLCSPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSQLTNEIPETGPVDNPDDPSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKELDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDALNKPPILYCSAK

Target ORF information:

RefSeq Version	XM_012967115.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis agrin (agrn), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967115.3

ORF Insert Sequence:

ATGACTGGCT GCCACTACCC TGCTCCAGGT TTGGAGAGGG ACCGGATCTA TCAGCATAAA 
GTCTCACTGG TTGTGCGCTA CTTCATGATC CCTTGTAACA TATGCCTGAT CCTGCTTGCC 
ACTTCCACGC TTGGATTTGC CGTCCTGCTG TTCCTGAATA ACTATAAACC TGGCCCTGTC 
CCTAATGCTC AGCCAACTCT ACAAGATGTG TGCCGAGGAA TGCTCTGCGG ATTTGGCGCA 
GTATGTGAGA AAAGCAGCAC CGACCCATCT CAGGCAACCT GCATCTGTAA GAAAACCGCC 
TGCGCCTCCA TTGTTGCCCC TGTGTGCGGT TCCGACTATT CTACTTACAG CAATGAGTGT 
GAACTGGAGA GAGCCCAGTG CAACCAGCAG AGGAGAATCA AAGTCATCAG CAAGGGGCCA 
TGCGGCACCA AGGACCCATG CTCAACAGTG TCGTGTAGCT ATGGCAGTAC CTGTGTCCGC 
TCAACAGATG GCCAGACGGC AAAGTGTATC TGTCCTGCTA CCTGCACAGG GGTTCCAGAA 
AATGTTGTTT GTGGCAGTGA CGGCAAAGAT TATCACAATG AGTGTCAGCT TAACAAGCAG 
GCATGCGACA ATCAGGAGAA TCTCTACAAG AAGTTTGATG GGCGTTGTGA TCCATGCAGA 
ATTGCCATGA ATAATCAAAA CAAAGCATGT CGAGTAAACC CACGAACTAG GCAACTGGAA 
ATGCAATACC GACCAGAATA CTGCCCAGTC CGCAGAGATC CTGTTTGCGC AGATGACGGT 
GTGACTTATG ACAATGATTG TGTAATGACT CGATCTGGAT TCATCCGTGG CATTGAACTG 
CAGAAGGTGC ACTCAGGGCA GTGCCAACCG CAAGACAAAT GCAAGGAGGA CTGTAAACAC 
AATTCTGTGT GTCTTAACCG GAGAGGTGTG ACGCGTTGTT CTTGCGACCG TGTTACTTGT 
GATAGCACTT ACAAACCAGT CTGTGGTGGG GACAGTCGCA CGTATGCCAA TGACTGCGAG 
AGACAAAAGG CAGAATGCTT TCAGAAAGCT ACCATTCCAG TAAAACACAA AGGCCCATGT 
GATCTCCATT CCCCCAGTCC ATGCCTGAAC ACAACCTGCA GTTTTGGAGC AGCATGTGTA 
GTAAAAAACA AGAAGGCAGT ATGCGAATGC CAGCAGGTGT GCCAGGCAGT ATATGACCCA 
GTGTGTGGCA GTGATAAGCG CACTTACGGC AACCCATGTG AACTGCAGTC AACAGCCTGC 
ACGCTTAAAA AAGAAATAAC CATTTTACAC AAGGGGCCTT GTGACCGCTG TGGGAAATGC 
CAGTTTGGAG CCATCTGTGA AGCAGAGACT GGCCGCTGTG TATGTCCAAC AGAATGTGTG 
CCTTCCGCTC AGCCTGTTTG TGGCACTGAT GGAAATACAT ACGAAAGCGA GTGTGAGCTC 
CATGTACGAG CATGCACTCA GCAGATTAAT CTACAAGTTG CAGCCCAAGG AGACTGCAAA 
ACCTGTGGCA ATACTCTTTG TACTTTTGGT GCCAAATGTG TGAACAACCA GTGTGTGTGC 
CTAACATGCG AGCGGCAACC CTACCTACCA GTGTGCGGCA GCAATGGCGT CACTTATGAT 
AACCAATGTG AACTGAAAGC TGCATCCTGT AAGCAGATGA AGCTGATTGA AGTATCTCGG 
GCAGGATCTT GTGAAGACGA ATGCGGCTCT GGCACTGACG GTTCTGGTTC TGGTGAGGAC 
AGCGAGTGTG AGCAGGATCG ATGCAGGCGC TATGGTGGCT CATGGGATGA AGATGCAGAA 
GATGATCGCT GCGTCTGTGA CTTTACCTGT CATGCTGTGC TTCGAATTTT GGTCTGTGGA 
TCGGATGGCA TTACCTACTC TAATGAGTGT GAGCTAAAGA AAACTCGGTG TGAGAAACGT 
CAGGATCTGT ATGTGGCTGG ACAGGGTGCC TGTCGAGCTG CCACCACCAG CTCTCCTCCT 
TTGGTGCCAC ACTGCAGTCA CACTGTGTAT GGCTGTTGTC CTGACAATAT CACAGCTGCC 
CTTGGCGTTG GCCTTGCCGG ATGCCCAAGC AGCTGTCAGT GCCACCAGTA TGGCTCCTAT 
GGAGGAACCT GTGATCCCAC CACTGGTCAG TGTTCTTGTA AACCAGGCGT GGGGGGACTG 
AAATGCGATA GATGTGAGCC CGGGTTTTGG AACTTCCGGG GCATTGTTAC GGATAAGAAA 
AGCGGCTGCA CCCCATGTAA CTGTGACCCA GTTGGTGCCG TTCGAGATGA CTGTGAGCAA 
ATGACAGGAC TTTGTTCTTG CAAAACGGGA ATCTCAGGTT TGAAGTGCAG CCAGTGCCCT 
GATGGACGCA AGCCTGGCCT TACAGGCTGT GAGAAAGATC CTTCAGCTCC TAAATCTTGT 
GATGCCATCA AGTGCCAATT TGGTGCAACT TGTGTGGTAA TTGGAGGATT TGCTCATTGC 
GAGTGCCCAT CTCCCCTGTG TGCTGAGGCG AATCTGACAA AGGTCTGTGG TTCCGATGAT 
GTGACTTACG GAGACTGGTG CCAACTGAAA ACCATTGCTT GCCGTCAGGG CCGCACTATC 
ACAGTGAAGC ACACTGGTCC GTGCCAAGAA ACTGTCACTC ATGCCAACCT TCAGACATTG 
AGTCCAACAG GAACACCCTC TCCGCCCATA GTTCGGACTC TTCCAACTCC AGGAACCACA 
AAGATAACAA AGACCAAGAA AAAGACAGAT TCCTTCCAGT TAGTTTTAGA AGCCACATCG 
CTCAGCCCAT TTTCCAGCAC CACAATGAGA CCTGTGACCC AGACGCATAG AGCTCACACT 
TCTACTGTCA GGCCAGCAAT CACTATTCCC ACAGTCCATC CCACTGTGCT TCAGATTTAT 
GATGAATCAG GAAGTGGAAG TGGGGATGAG GAGCTAGAAG CTAGCGGTGA TCAAGATTTA 
AGTGGTGTTG AACCCAAAGA GCCATTTGAT GTGGAGAATG AACAGAAGGA AGAAGCTGAA 
GAGAAGAGAG ATTTTGTAGC CACCACGGTC ATAGAAAGAG CCAGCTGCTA TAACACACCT 
CTAGGATGCT GCTCTGATGG AAAGACCCCA GCTAATGATG CTGAAGGAAC AAATTGCCCA 
AAAACCAAAA TTTTCCAAGG TGTCCTCATT ATTGAAGAGT TGGAGGGGCA AGAGGTTTTC 
TACACACCAG AAATGTCAGA TTACAAGTCT GAGCTCTTTG GGGAAACTGC TAGAAGCATT 
GAGAATGCAC TGGATAATCT ACTTCGAAAT TCAGACATCA AGAGGGATTT CAAGAGCATC 
CGTGTGAGGG ACCTTGGACC AAGCAATTCC ATCAGAGCCA TTGTGGAAGC ACATTTTGAT 
CCAGCTACCA CCATGTCTGC TATGGATGTT CAGAGAGCAT TGCTGCGACA GCTCAAGAGC 
TCCAAGAAGA GAGCAATAAC CATAAAGAAG CCTATTCAGG AAAATGTCAA GTTCCTGGAC 
TTTGGTGAGA TCCCTGACTG GGTGCCTCTA ATGGTTACAA CCACAACTAC AACGGCAGCA 
ACCACTAGCA TGGTAGCTAC TACTGCTGTT GCCCCCACCA CCACAACCCC TGTAGTCGCT 
ACCACAACAA CTGTGGCAAG AACTCCACTA CCTAGAAGAA TAGTTGCAGT CAAGACCACC 
CCTGTGACAA TCACTCAGCA ACCTGTCACC ACTCCAAGAA TGACAACAAG ACGACCAGTA 
ACTACAAAAA GACCAATGAC CACTATTCCT GCCACCACCA GGAGAAAGCC ACCACGTATT 
CAAGCAGTCA CAAAAAAGCC ACCCCGGCCA TGTGAATCAC ACCCTTGTCT ACATGGAGGT 
ACTTGTGAGG ATAATGGGAA GGATTTCACC TGTAGCTGCC CAGCTGGCAG AGGAGGAGCT 
GTGTGCGAGA AGAGTATTAA GTATTTTATT CCTGGGTTTG GTGGTAAGTC ATACCTGGCA 
TTCAAGATGA TGAAGGCATA TCATACAGTG CGCATAGCCA TGGAGTTCCG ATCCTCTGAG 
CAGAACGGGC TTCTCCTATA CAATGGTCAG TCACTTGGGA AGGACTTTAT CTCACTTGCA 
GTTAATAATG GCTTTGTGGA ACTCAGGTTT AATACTGGAT CTGGAACTGG AATAATAACT 
AGTAAAGTGC CCATTGAACC TGGCAAGTGG CATCAGTTAG TAGTGAACCG TAACAGACGC 
AGTGGCATGT TGTCTGTGGA TGGTGAATCC CATGTCAATG GTGAAAGTCC ATCTGGAACC 
GATGGCCTCA ACCTAGACAC AGACCTGTTT ATAGGAGGAG CTCCTGAGGA CCAGATGGCT 
GTGGTATCAG AAAGGACCTC TTTGTCTGTG GGGCTTAAAG GCTGCATCCG ACAACTTGAT 
GTGAACAACC AACTATACAA CCTTCAAGAG GGAGGGGCTG ATGTCTTGTA TGGAAGTGGG 
GTGGGAGAGT GTGGAAACAA CCCTTGCCAG CCCAACCCAT GCCATAATGG TGCTGCATGC 
CAAGTAAAGG AGGCTGAAAT GTTCCACTGC CAGTGTCTTA GTGGTTATAC AGGGCCAACC 
TGTGCAGATG AACGGAACCC ATGTGACCCC AACCCCTGCC ACGTTTCTGC TACCTGTTTG 
GTTCTACCTG AGGGAGGTGC TAAATGTGAA TGTCCAATGG GCAGAGAGGG AGAGTTTTGT 
GAGAAAGTGA GCAACCAGGA TCTGAATTTG CCATTCTTAC CTCAGTTTAA TGGATACTCT 
TACTTGGAAA TGAAGGGACT ACAGACATTT GCCACTGACC TACAGGACAA GCTTGCCATG 
GAAGTAGTGT TCCTAGCCAG CAACCCCAAT GGCCTCATCT TCTATAATGG GCAGAAAACT 
GATGGAAAAG GAGATTTTGT GTCACTGTCC CTTCATGATG GCAATCTAGA ATACCGTTAC 
GATCTCGGAA AGGGAGCTGC TGTCATCAAG AGTAAGGAAA AGATTCCACT CAACACATGG 
CTCAGCGTCT CCCTTGAGCG TAGCGGCAGG AAGGGATTGA TGCGGATCAA TAACATGGAA 
CAAGTTACTG GGGAATCACC GAAATCCCGT AAGGCACCAC ATACCGCTCT CAATCTGAAA 
GAGCCTCTAT TTGTGGGTGG AGCTCCAGAT TTCAGCAAAT TAGCCAGGGC AGCGGCTGCC 
TCTACAAGCT TTGAAGGAGC CATTCAGAAG ATTTCCATTA AGGGGCTGCC TGTTTTGAGA 
GAAGAAAACA TCCGCAGTGC CATTGAGATC TCTACTTTCC GCAGCCATCC TTGTACCAAG 
AAACCCTTCC CTTGCCAGAA TGGGGGGCTT TGTTCTCCTA ATAAAGGCAA CTATGAGTGT 
GTTTGCCAGA GGGGTTTCTC AGGCAGCCAG TGTGAAAAAG TGACCATTGA GAAAGCAGCT 
GGAGAGTCTG AGGCAGTATC ATTTGATGGC AAGACTTACT TGGAATATCA CATTGCTGTA 
ACCAAAAGCC AACTAACTAA CGAGATACCT GAAACTGGTC CTGTGGACAA TCCAGATGAC 
CCCAGTGAAA AGGCGCTGCT AGTCAACCAA TTTGAGCTGA GCATCAAGAC TGAAGCCACT 
CAGGGTCTCA TCCTGTGGAG CGGGAAAGGG ACAGAACGTG CCTATTATAT TGCCTTGGCT 
GTAGTTGGAG GTTATGTTGA GATGACCTAT GACCTGGGCT CCAAGCCTGT TGTCCTACGA 
TCCACAGTAC CTGTGAACAC CAACGAGTGG ATTCACATCA AAGCTGTACG AGACAACCGA 
GATGGCATTT TACGAGTTGG CAATGAGGAC CCCGTCCATG GATCCTCTCC TTTTGGAGTC 
AAAGAGCTGG ACACTGATGG AGCCCTATGG CTGGGTGGAA TAGAAAAGCT GAGTGCCTCA 
TACAAGCTGC CCAAAGCGTA CAGCACGGGA TTCATTGGCT GCATGAAGGA CGTTCTGGTG 
GACCAACAAG ACCTGCACTT AGTGGAAGAT GCATTGAATA AACCACCCAT ATTGTACTGC 
TCAGCCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agrn ( XM_018096012.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951501.1 Xenopus tropicalis agrin (agrn), transcript variant X1, mRNA.

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CloneID	OXa45875
Clone ID Related Accession (Same CDS sequence)	XM_018096012.2 , XM_018096012.1
Accession Version	XM_018096012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	agrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGGTGAGATC CCTGACTGGG TGCCTCTAAT GGTTACAACC 
ACAACTACAA CGGCAGCAAC CACTAGCATG GTAGCTACTA CTGCTGTTGC CCCCACCACC 
ACAACCCCTG TAGTCGCTAC CACAACAACT GTGGCAAGAA CTCCACTACC TAGAAGAATA 
GTTGCAGTCA AGACCACCCC TGTGACAATC ACTCAGCAAC CTGTCACCAC TCCAAGAATG 
ACAACAAGAC GACCAGTAAC TACAAAAAGA CCAATGACCA CTATTCCTGC CACCACCAGG 
AGAAAGCCAC CACGTATTCA AGCAGTCACA AAAAAGCCAC CCCGGCCATG TGAATCACAC 
CCTTGTCTAC ATGGAGGTAC TTGTGAGGAT AATGGGAAGG ATTTCACCTG TAGCTGCCCA 
GCTGGCAGAG GAGGAGCTGT GTGCGAGAAG AGTATTAAGT ATTTTATTCC TGGGTTTGGT 
GGTAAGTCAT ACCTGGCATT CAAGATGATG AAGGCATATC ATACAGTGCG CATAGCCATG 
GAGTTCCGAT CCTCTGAGCA GAACGGGCTT CTCCTATACA ATGGTCAGTC ACTTGGGAAG 
GACTTTATCT CACTTGCAGT TAATAATGGC TTTGTGGAAC TCAGGTTTAA TACTGGATCT 
GGAACTGGAA TAATAACTAG TAAAGTGCCC ATTGAACCTG GCAAGTGGCA TCAGTTAGTA 
GTGAACCGTA ACAGACGCAG TGGCATGTTG TCTGTGGATG GTGAATCCCA TGTCAATGGT 
GAAAGTCCAT CTGGAACCGA TGGCCTCAAC CTAGACACAG ACCTGTTTAT AGGAGGAGCT 
CCTGAGGACC AGATGGCTGT GGTATCAGAA AGGACCTCTT TGTCTGTGGG GCTTAAAGGC 
TGCATCCGAC AACTTGATGT GAACAACCAA CTATACAACC TTCAAGAGGG AGGGGCTGAT 
GTCTTGTATG GAAGTGGGGT GGGAGAGTGT GGAAACAACC CTTGCCAGCC CAACCCATGC 
CATAATGGTG CTGCATGCCA AGTAAAGGAG GCTGAAATGT TCCACTGCCA GTGTCTTAGT 
GGTTATACAG GGCCAACCTG TGCAGATGAA CGGAACCCAT GTGACCCCAA CCCCTGCCAC 
GTTTCTGCTA CCTGTTTGGT TCTACCTGAG GGAGGTGCTA AATGTGAATG TCCAATGGGC 
AGAGAGGGAG AGTTTTGTGA GAAAGTGAGC AACCAGGATC TGAATTTGCC ATTCTTACCT 
CAGTTTAATG GATACTCTTA CTTGGAAATG AAGGGACTAC AGACATTTGC CACTGACCTA 
CAGGACAAGC TTGCCATGGA AGTAGTGTTC CTAGCCAGCA ACCCCAATGG CCTCATCTTC 
TATAATGGGC AGAAAACTGA TGGAAAAGGA GATTTTGTGT CACTGTCCCT TCATGATGGC 
AATCTAGAAT ACCGTTACGA TCTCGGAAAG GGAGCTGCTG TCATCAAGAG TAAGGAAAAG 
ATTCCACTCA ACACATGGCT CAGCGTCTCC CTTGAGCGTA GCGGCAGGAA GGGATTGATG 
CGGATCAATA ACATGGAACA AGTTACTGGG GAATCACCGA AATCCCGTAA GGCACCACAT 
ACCGCTCTCA ATCTGAAAGA GCCTCTATTT GTGGGTGGAG CTCCAGATTT CAGCAAATTA 
GCCAGGGCAG CGGCTGCCTC TACAAGCTTT GAAGGAGCCA TTCAGAAGAT TTCCATTAAG 
GGGCTGCCTG TTTTGAGAGA AGAAAACATC CGCAGTGCCA TTGAGATCTC TACTTTCCGC 
AGCCATCCTT GTACCAAGAA ACCCTTCCCT TGCCAGAATG GGGGGCTTTG TTCTCCTAAT 
AAAGGCAACT ATGAGTGTGT TTGCCAGAGG GGTTTCTCAG GCAGCCAGTG TGAAAAAGTG 
ACCATTGAGA AAGCAGCTGG AGAGTCTGAG GCAGTATCAT TTGATGGCAA GACTTACTTG 
GAATATCACA TTGCTGTAAC CAAAAGCCAA CTAACTAACG AGATACCTGA AACTGGTCCT 
GTGGACAATC CAGATGACCC CAGTGAAAAG GCGCTGCTAG TCAACCAATT TGAGCTGAGC 
ATCAAGACTG AAGCCACTCA GGGTCTCATC CTGTGGAGCG GGAAAGGGAC AGAACGTGCC 
TATTATATTG CCTTGGCTGT AGTTGGAGGT TATGTTGAGA TGACCTATGA CCTGGGCTCC 
AAGCCTGTTG TCCTACGATC CACAGTACCT GTGAACACCA ACGAGTGGAT TCACATCAAA 
GCTGTACGAG ACAACCGAGA TGGCATTTTA CGAGTTGGCA ATGAGGACCC CGTCCATGGA 
TCCTCTCCTT TTGGAGTCAA AGAGCTGGAC ACTGATGGAG CCCTATGGCT GGGTGGAATA 
GAAAAGCTGA GTGCCTCATA CAAGCTGCCC AAAGCGTACA GCACGGGATT CATTGGCTGC 
ATGAAGGACG TTCTGGTGGA CCAACAAGAC CTGCACTTAG TGGAAGATGC ATTGAATAAA 
CCACCCATAT TGTACTGCTC AGCCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951501.1
CDS	304..6633
Translation	MGRRWGAPAVLLWTLWALAAVGLGFSRGPCPERELELREEEANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNKVMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEEHRRLSADKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACASIVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDPCSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRVNPRTRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQDKCKEDCKHNSVCLNRRGVTRCSCDRVTCDSTYKPVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCSFGAACVVKNKKAVCECQQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGAICEAETGRCVCPTECVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKCVNNQCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETVTHANLQTLSPTGTPSPPIVRTLPTPGTTKITKTKKKTDSFQLVLEATSLSPFSSTTMRPVTQTHRAHTSTVRPAITIPTVHPTVLQIYDESGSGSGDEELEASGDQDLSGVEPKEPFDVENEQKEEAEEKRDFVATTVIERASCYNTPLGCCSDGKTPANDAEGTNCPKTKIFQGVLIIEELEGQEVFYTPEMSDYKSELFGETARSIENALDNLLRNSDIKRDFKSIRVRDLGPSNSIRAIVEAHFDPATTMSAMDVQRALLRQLKSSKKRAITIKKPIQENVKFLDFGEIPDWVPLMVTTTTTTAATTSMVATTAVAPTTTTPVVATTTTVARTPLPRRIVAVKTTPVTITQQPVTTPRMTTRRPVTTKRPMTTIPATTRRKPPRIQAVTKKPPRPCESHPCLHGGTCEDNGKDFTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNLKEPLFVGGAPDFSKLARAAAASTSFEGAIQKISIKGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLCSPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSQLTNEIPETGPVDNPDDPSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKELDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDALNKPPILYCSAK

Target ORF information:

RefSeq Version	XM_018096012.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis agrin (agrn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096012.2

ORF Insert Sequence:

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGGTGAGATC CCTGACTGGG TGCCTCTAAT GGTTACAACC 
ACAACTACAA CGGCAGCAAC CACTAGCATG GTAGCTACTA CTGCTGTTGC CCCCACCACC 
ACAACCCCTG TAGTCGCTAC CACAACAACT GTGGCAAGAA CTCCACTACC TAGAAGAATA 
GTTGCAGTCA AGACCACCCC TGTGACAATC ACTCAGCAAC CTGTCACCAC TCCAAGAATG 
ACAACAAGAC GACCAGTAAC TACAAAAAGA CCAATGACCA CTATTCCTGC CACCACCAGG 
AGAAAGCCAC CACGTATTCA AGCAGTCACA AAAAAGCCAC CCCGGCCATG TGAATCACAC 
CCTTGTCTAC ATGGAGGTAC TTGTGAGGAT AATGGGAAGG ATTTCACCTG TAGCTGCCCA 
GCTGGCAGAG GAGGAGCTGT GTGCGAGAAG AGTATTAAGT ATTTTATTCC TGGGTTTGGT 
GGTAAGTCAT ACCTGGCATT CAAGATGATG AAGGCATATC ATACAGTGCG CATAGCCATG 
GAGTTCCGAT CCTCTGAGCA GAACGGGCTT CTCCTATACA ATGGTCAGTC ACTTGGGAAG 
GACTTTATCT CACTTGCAGT TAATAATGGC TTTGTGGAAC TCAGGTTTAA TACTGGATCT 
GGAACTGGAA TAATAACTAG TAAAGTGCCC ATTGAACCTG GCAAGTGGCA TCAGTTAGTA 
GTGAACCGTA ACAGACGCAG TGGCATGTTG TCTGTGGATG GTGAATCCCA TGTCAATGGT 
GAAAGTCCAT CTGGAACCGA TGGCCTCAAC CTAGACACAG ACCTGTTTAT AGGAGGAGCT 
CCTGAGGACC AGATGGCTGT GGTATCAGAA AGGACCTCTT TGTCTGTGGG GCTTAAAGGC 
TGCATCCGAC AACTTGATGT GAACAACCAA CTATACAACC TTCAAGAGGG AGGGGCTGAT 
GTCTTGTATG GAAGTGGGGT GGGAGAGTGT GGAAACAACC CTTGCCAGCC CAACCCATGC 
CATAATGGTG CTGCATGCCA AGTAAAGGAG GCTGAAATGT TCCACTGCCA GTGTCTTAGT 
GGTTATACAG GGCCAACCTG TGCAGATGAA CGGAACCCAT GTGACCCCAA CCCCTGCCAC 
GTTTCTGCTA CCTGTTTGGT TCTACCTGAG GGAGGTGCTA AATGTGAATG TCCAATGGGC 
AGAGAGGGAG AGTTTTGTGA GAAAGTGAGC AACCAGGATC TGAATTTGCC ATTCTTACCT 
CAGTTTAATG GATACTCTTA CTTGGAAATG AAGGGACTAC AGACATTTGC CACTGACCTA 
CAGGACAAGC TTGCCATGGA AGTAGTGTTC CTAGCCAGCA ACCCCAATGG CCTCATCTTC 
TATAATGGGC AGAAAACTGA TGGAAAAGGA GATTTTGTGT CACTGTCCCT TCATGATGGC 
AATCTAGAAT ACCGTTACGA TCTCGGAAAG GGAGCTGCTG TCATCAAGAG TAAGGAAAAG 
ATTCCACTCA ACACATGGCT CAGCGTCTCC CTTGAGCGTA GCGGCAGGAA GGGATTGATG 
CGGATCAATA ACATGGAACA AGTTACTGGG GAATCACCGA AATCCCGTAA GGCACCACAT 
ACCGCTCTCA ATCTGAAAGA GCCTCTATTT GTGGGTGGAG CTCCAGATTT CAGCAAATTA 
GCCAGGGCAG CGGCTGCCTC TACAAGCTTT GAAGGAGCCA TTCAGAAGAT TTCCATTAAG 
GGGCTGCCTG TTTTGAGAGA AGAAAACATC CGCAGTGCCA TTGAGATCTC TACTTTCCGC 
AGCCATCCTT GTACCAAGAA ACCCTTCCCT TGCCAGAATG GGGGGCTTTG TTCTCCTAAT 
AAAGGCAACT ATGAGTGTGT TTGCCAGAGG GGTTTCTCAG GCAGCCAGTG TGAAAAAGTG 
ACCATTGAGA AAGCAGCTGG AGAGTCTGAG GCAGTATCAT TTGATGGCAA GACTTACTTG 
GAATATCACA TTGCTGTAAC CAAAAGCCAA CTAACTAACG AGATACCTGA AACTGGTCCT 
GTGGACAATC CAGATGACCC CAGTGAAAAG GCGCTGCTAG TCAACCAATT TGAGCTGAGC 
ATCAAGACTG AAGCCACTCA GGGTCTCATC CTGTGGAGCG GGAAAGGGAC AGAACGTGCC 
TATTATATTG CCTTGGCTGT AGTTGGAGGT TATGTTGAGA TGACCTATGA CCTGGGCTCC 
AAGCCTGTTG TCCTACGATC CACAGTACCT GTGAACACCA ACGAGTGGAT TCACATCAAA 
GCTGTACGAG ACAACCGAGA TGGCATTTTA CGAGTTGGCA ATGAGGACCC CGTCCATGGA 
TCCTCTCCTT TTGGAGTCAA AGAGCTGGAC ACTGATGGAG CCCTATGGCT GGGTGGAATA 
GAAAAGCTGA GTGCCTCATA CAAGCTGCCC AAAGCGTACA GCACGGGATT CATTGGCTGC 
ATGAAGGACG TTCTGGTGGA CCAACAAGAC CTGCACTTAG TGGAAGATGC ATTGAATAAA 
CCACCCATAT TGTACTGCTC AGCCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agrn ( XM_018096012.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951501.1 Xenopus tropicalis agrin (agrn), transcript variant X1, mRNA.

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CloneID	OXa45875
Clone ID Related Accession (Same CDS sequence)	XM_018096012.2 , XM_018096012.1
Accession Version	XM_018096012.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	agrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGGTGAGATC CCTGACTGGG TGCCTCTAAT GGTTACAACC 
ACAACTACAA CGGCAGCAAC CACTAGCATG GTAGCTACTA CTGCTGTTGC CCCCACCACC 
ACAACCCCTG TAGTCGCTAC CACAACAACT GTGGCAAGAA CTCCACTACC TAGAAGAATA 
GTTGCAGTCA AGACCACCCC TGTGACAATC ACTCAGCAAC CTGTCACCAC TCCAAGAATG 
ACAACAAGAC GACCAGTAAC TACAAAAAGA CCAATGACCA CTATTCCTGC CACCACCAGG 
AGAAAGCCAC CACGTATTCA AGCAGTCACA AAAAAGCCAC CCCGGCCATG TGAATCACAC 
CCTTGTCTAC ATGGAGGTAC TTGTGAGGAT AATGGGAAGG ATTTCACCTG TAGCTGCCCA 
GCTGGCAGAG GAGGAGCTGT GTGCGAGAAG AGTATTAAGT ATTTTATTCC TGGGTTTGGT 
GGTAAGTCAT ACCTGGCATT CAAGATGATG AAGGCATATC ATACAGTGCG CATAGCCATG 
GAGTTCCGAT CCTCTGAGCA GAACGGGCTT CTCCTATACA ATGGTCAGTC ACTTGGGAAG 
GACTTTATCT CACTTGCAGT TAATAATGGC TTTGTGGAAC TCAGGTTTAA TACTGGATCT 
GGAACTGGAA TAATAACTAG TAAAGTGCCC ATTGAACCTG GCAAGTGGCA TCAGTTAGTA 
GTGAACCGTA ACAGACGCAG TGGCATGTTG TCTGTGGATG GTGAATCCCA TGTCAATGGT 
GAAAGTCCAT CTGGAACCGA TGGCCTCAAC CTAGACACAG ACCTGTTTAT AGGAGGAGCT 
CCTGAGGACC AGATGGCTGT GGTATCAGAA AGGACCTCTT TGTCTGTGGG GCTTAAAGGC 
TGCATCCGAC AACTTGATGT GAACAACCAA CTATACAACC TTCAAGAGGG AGGGGCTGAT 
GTCTTGTATG GAAGTGGGGT GGGAGAGTGT GGAAACAACC CTTGCCAGCC CAACCCATGC 
CATAATGGTG CTGCATGCCA AGTAAAGGAG GCTGAAATGT TCCACTGCCA GTGTCTTAGT 
GGTTATACAG GGCCAACCTG TGCAGATGAA CGGAACCCAT GTGACCCCAA CCCCTGCCAC 
GTTTCTGCTA CCTGTTTGGT TCTACCTGAG GGAGGTGCTA AATGTGAATG TCCAATGGGC 
AGAGAGGGAG AGTTTTGTGA GAAAGTGAGC AACCAGGATC TGAATTTGCC ATTCTTACCT 
CAGTTTAATG GATACTCTTA CTTGGAAATG AAGGGACTAC AGACATTTGC CACTGACCTA 
CAGGACAAGC TTGCCATGGA AGTAGTGTTC CTAGCCAGCA ACCCCAATGG CCTCATCTTC 
TATAATGGGC AGAAAACTGA TGGAAAAGGA GATTTTGTGT CACTGTCCCT TCATGATGGC 
AATCTAGAAT ACCGTTACGA TCTCGGAAAG GGAGCTGCTG TCATCAAGAG TAAGGAAAAG 
ATTCCACTCA ACACATGGCT CAGCGTCTCC CTTGAGCGTA GCGGCAGGAA GGGATTGATG 
CGGATCAATA ACATGGAACA AGTTACTGGG GAATCACCGA AATCCCGTAA GGCACCACAT 
ACCGCTCTCA ATCTGAAAGA GCCTCTATTT GTGGGTGGAG CTCCAGATTT CAGCAAATTA 
GCCAGGGCAG CGGCTGCCTC TACAAGCTTT GAAGGAGCCA TTCAGAAGAT TTCCATTAAG 
GGGCTGCCTG TTTTGAGAGA AGAAAACATC CGCAGTGCCA TTGAGATCTC TACTTTCCGC 
AGCCATCCTT GTACCAAGAA ACCCTTCCCT TGCCAGAATG GGGGGCTTTG TTCTCCTAAT 
AAAGGCAACT ATGAGTGTGT TTGCCAGAGG GGTTTCTCAG GCAGCCAGTG TGAAAAAGTG 
ACCATTGAGA AAGCAGCTGG AGAGTCTGAG GCAGTATCAT TTGATGGCAA GACTTACTTG 
GAATATCACA TTGCTGTAAC CAAAAGCCAA CTAACTAACG AGATACCTGA AACTGGTCCT 
GTGGACAATC CAGATGACCC CAGTGAAAAG GCGCTGCTAG TCAACCAATT TGAGCTGAGC 
ATCAAGACTG AAGCCACTCA GGGTCTCATC CTGTGGAGCG GGAAAGGGAC AGAACGTGCC 
TATTATATTG CCTTGGCTGT AGTTGGAGGT TATGTTGAGA TGACCTATGA CCTGGGCTCC 
AAGCCTGTTG TCCTACGATC CACAGTACCT GTGAACACCA ACGAGTGGAT TCACATCAAA 
GCTGTACGAG ACAACCGAGA TGGCATTTTA CGAGTTGGCA ATGAGGACCC CGTCCATGGA 
TCCTCTCCTT TTGGAGTCAA AGAGCTGGAC ACTGATGGAG CCCTATGGCT GGGTGGAATA 
GAAAAGCTGA GTGCCTCATA CAAGCTGCCC AAAGCGTACA GCACGGGATT CATTGGCTGC 
ATGAAGGACG TTCTGGTGGA CCAACAAGAC CTGCACTTAG TGGAAGATGC ATTGAATAAA 
CCACCCATAT TGTACTGCTC AGCCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951501.1
CDS	679..7008
Translation	MGRRWGAPAVLLWTLWALAAVGLGFSRGPCPERELELREEEANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNKVMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEEHRRLSADKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACASIVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDPCSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRVNPRTRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQDKCKEDCKHNSVCLNRRGVTRCSCDRVTCDSTYKPVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCSFGAACVVKNKKAVCECQQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGAICEAETGRCVCPTECVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKCVNNQCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETVTHANLQTLSPTGTPSPPIVRTLPTPGTTKITKTKKKTDSFQLVLEATSLSPFSSTTMRPVTQTHRAHTSTVRPAITIPTVHPTVLQIYDESGSGSGDEELEASGDQDLSGVEPKEPFDVENEQKEEAEEKRDFVATTVIERASCYNTPLGCCSDGKTPANDAEGTNCPKTKIFQGVLIIEELEGQEVFYTPEMSDYKSELFGETARSIENALDNLLRNSDIKRDFKSIRVRDLGPSNSIRAIVEAHFDPATTMSAMDVQRALLRQLKSSKKRAITIKKPIQENVKFLDFGEIPDWVPLMVTTTTTTAATTSMVATTAVAPTTTTPVVATTTTVARTPLPRRIVAVKTTPVTITQQPVTTPRMTTRRPVTTKRPMTTIPATTRRKPPRIQAVTKKPPRPCESHPCLHGGTCEDNGKDFTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNLKEPLFVGGAPDFSKLARAAAASTSFEGAIQKISIKGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLCSPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSQLTNEIPETGPVDNPDDPSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKELDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDALNKPPILYCSAK

Target ORF information:

RefSeq Version	XM_018096012.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis agrin (agrn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096012.1

ORF Insert Sequence:

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGGTGAGATC CCTGACTGGG TGCCTCTAAT GGTTACAACC 
ACAACTACAA CGGCAGCAAC CACTAGCATG GTAGCTACTA CTGCTGTTGC CCCCACCACC 
ACAACCCCTG TAGTCGCTAC CACAACAACT GTGGCAAGAA CTCCACTACC TAGAAGAATA 
GTTGCAGTCA AGACCACCCC TGTGACAATC ACTCAGCAAC CTGTCACCAC TCCAAGAATG 
ACAACAAGAC GACCAGTAAC TACAAAAAGA CCAATGACCA CTATTCCTGC CACCACCAGG 
AGAAAGCCAC CACGTATTCA AGCAGTCACA AAAAAGCCAC CCCGGCCATG TGAATCACAC 
CCTTGTCTAC ATGGAGGTAC TTGTGAGGAT AATGGGAAGG ATTTCACCTG TAGCTGCCCA 
GCTGGCAGAG GAGGAGCTGT GTGCGAGAAG AGTATTAAGT ATTTTATTCC TGGGTTTGGT 
GGTAAGTCAT ACCTGGCATT CAAGATGATG AAGGCATATC ATACAGTGCG CATAGCCATG 
GAGTTCCGAT CCTCTGAGCA GAACGGGCTT CTCCTATACA ATGGTCAGTC ACTTGGGAAG 
GACTTTATCT CACTTGCAGT TAATAATGGC TTTGTGGAAC TCAGGTTTAA TACTGGATCT 
GGAACTGGAA TAATAACTAG TAAAGTGCCC ATTGAACCTG GCAAGTGGCA TCAGTTAGTA 
GTGAACCGTA ACAGACGCAG TGGCATGTTG TCTGTGGATG GTGAATCCCA TGTCAATGGT 
GAAAGTCCAT CTGGAACCGA TGGCCTCAAC CTAGACACAG ACCTGTTTAT AGGAGGAGCT 
CCTGAGGACC AGATGGCTGT GGTATCAGAA AGGACCTCTT TGTCTGTGGG GCTTAAAGGC 
TGCATCCGAC AACTTGATGT GAACAACCAA CTATACAACC TTCAAGAGGG AGGGGCTGAT 
GTCTTGTATG GAAGTGGGGT GGGAGAGTGT GGAAACAACC CTTGCCAGCC CAACCCATGC 
CATAATGGTG CTGCATGCCA AGTAAAGGAG GCTGAAATGT TCCACTGCCA GTGTCTTAGT 
GGTTATACAG GGCCAACCTG TGCAGATGAA CGGAACCCAT GTGACCCCAA CCCCTGCCAC 
GTTTCTGCTA CCTGTTTGGT TCTACCTGAG GGAGGTGCTA AATGTGAATG TCCAATGGGC 
AGAGAGGGAG AGTTTTGTGA GAAAGTGAGC AACCAGGATC TGAATTTGCC ATTCTTACCT 
CAGTTTAATG GATACTCTTA CTTGGAAATG AAGGGACTAC AGACATTTGC CACTGACCTA 
CAGGACAAGC TTGCCATGGA AGTAGTGTTC CTAGCCAGCA ACCCCAATGG CCTCATCTTC 
TATAATGGGC AGAAAACTGA TGGAAAAGGA GATTTTGTGT CACTGTCCCT TCATGATGGC 
AATCTAGAAT ACCGTTACGA TCTCGGAAAG GGAGCTGCTG TCATCAAGAG TAAGGAAAAG 
ATTCCACTCA ACACATGGCT CAGCGTCTCC CTTGAGCGTA GCGGCAGGAA GGGATTGATG 
CGGATCAATA ACATGGAACA AGTTACTGGG GAATCACCGA AATCCCGTAA GGCACCACAT 
ACCGCTCTCA ATCTGAAAGA GCCTCTATTT GTGGGTGGAG CTCCAGATTT CAGCAAATTA 
GCCAGGGCAG CGGCTGCCTC TACAAGCTTT GAAGGAGCCA TTCAGAAGAT TTCCATTAAG 
GGGCTGCCTG TTTTGAGAGA AGAAAACATC CGCAGTGCCA TTGAGATCTC TACTTTCCGC 
AGCCATCCTT GTACCAAGAA ACCCTTCCCT TGCCAGAATG GGGGGCTTTG TTCTCCTAAT 
AAAGGCAACT ATGAGTGTGT TTGCCAGAGG GGTTTCTCAG GCAGCCAGTG TGAAAAAGTG 
ACCATTGAGA AAGCAGCTGG AGAGTCTGAG GCAGTATCAT TTGATGGCAA GACTTACTTG 
GAATATCACA TTGCTGTAAC CAAAAGCCAA CTAACTAACG AGATACCTGA AACTGGTCCT 
GTGGACAATC CAGATGACCC CAGTGAAAAG GCGCTGCTAG TCAACCAATT TGAGCTGAGC 
ATCAAGACTG AAGCCACTCA GGGTCTCATC CTGTGGAGCG GGAAAGGGAC AGAACGTGCC 
TATTATATTG CCTTGGCTGT AGTTGGAGGT TATGTTGAGA TGACCTATGA CCTGGGCTCC 
AAGCCTGTTG TCCTACGATC CACAGTACCT GTGAACACCA ACGAGTGGAT TCACATCAAA 
GCTGTACGAG ACAACCGAGA TGGCATTTTA CGAGTTGGCA ATGAGGACCC CGTCCATGGA 
TCCTCTCCTT TTGGAGTCAA AGAGCTGGAC ACTGATGGAG CCCTATGGCT GGGTGGAATA 
GAAAAGCTGA GTGCCTCATA CAAGCTGCCC AAAGCGTACA GCACGGGATT CATTGGCTGC 
ATGAAGGACG TTCTGGTGGA CCAACAAGAC CTGCACTTAG TGGAAGATGC ATTGAATAAA 
CCACCCATAT TGTACTGCTC AGCCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agrn ( XM_018096013.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951502.1 Xenopus tropicalis agrin (agrn), transcript variant X2, mRNA.

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CloneID	OXa45876
Clone ID Related Accession (Same CDS sequence)	XM_018096013.1 , XM_018096013.2
Accession Version	XM_018096013.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	agrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGGTGAGATC CCTGACTGGG TGCCTCTAAT GGTTACAACC 
ACAACTACAA CGGCAGCAAC CACTAGCATG GTAGCTACTA CTGCTGTTGC CCCCACCACC 
ACAACCCCTG TAGTCGCTAC CACAACAACT GTGGCAAGAA CTCCACTACC TAGAAGAATA 
GTTGCAGTCA AGACCACCCC TGTGACAATC ACTCAGCAAC CTGTCACCAC TCCAAGAATG 
ACAACAAGAC GACCAGTAAC TACAAAAAGA CCAATGACCA CTATTCCTGC CACCACCAGG 
AGAAAGCCAC CACGTATTCA AGCAGTCACA AAAAAGCCAC CCCGGCCATG TGAATCACAC 
CCTTGTCTAC ATGGAGGTAC TTGTGAGGAT AATGGGAAGG ATTTCACCTG TAGCTGCCCA 
GCTGGCAGAG GAGGAGCTGT GTGCGAGAAG AGTATTAAGT ATTTTATTCC TGGGTTTGGT 
GGTAAGTCAT ACCTGGCATT CAAGATGATG AAGGCATATC ATACAGTGCG CATAGCCATG 
GAGTTCCGAT CCTCTGAGCA GAACGGGCTT CTCCTATACA ATGGTCAGTC ACTTGGGAAG 
GACTTTATCT CACTTGCAGT TAATAATGGC TTTGTGGAAC TCAGGTTTAA TACTGGATCT 
GGAACTGGAA TAATAACTAG TAAAGTGCCC ATTGAACCTG GCAAGTGGCA TCAGTTAGTA 
GTGAACCGTA ACAGACGCAG TGGCATGTTG TCTGTGGATG GTGAATCCCA TGTCAATGGT 
GAAAGTCCAT CTGGAACCGA TGGCCTCAAC CTAGACACAG ACCTGTTTAT AGGAGGAGCT 
CCTGAGGACC AGATGGCTGT GGTATCAGAA AGGACCTCTT TGTCTGTGGG GCTTAAAGGC 
TGCATCCGAC AACTTGATGT GAACAACCAA CTATACAACC TTCAAGAGGG AGGGGCTGAT 
GTCTTGTATG GAAGTGGGGT GGGAGAGTGT GGAAACAACC CTTGCCAGCC CAACCCATGC 
CATAATGGTG CTGCATGCCA AGTAAAGGAG GCTGAAATGT TCCACTGCCA GTGTCTTAGT 
GGTTATACAG GGCCAACCTG TGCAGATGAA CGGAACCCAT GTGACCCCAA CCCCTGCCAC 
GTTTCTGCTA CCTGTTTGGT TCTACCTGAG GGAGGTGCTA AATGTGAATG TCCAATGGGC 
AGAGAGGGAG AGTTTTGTGA GAAAGTGAGC AACCAGGATC TGAATTTGCC ATTCTTACCT 
CAGTTTAATG GATACTCTTA CTTGGAAATG AAGGGACTAC AGACATTTGC CACTGACCTA 
CAGGACAAGC TTGCCATGGA AGTAGTGTTC CTAGCCAGCA ACCCCAATGG CCTCATCTTC 
TATAATGGGC AGAAAACTGA TGGAAAAGGA GATTTTGTGT CACTGTCCCT TCATGATGGC 
AATCTAGAAT ACCGTTACGA TCTCGGAAAG GGAGCTGCTG TCATCAAGAG TAAGGAAAAG 
ATTCCACTCA ACACATGGCT CAGCGTCTCC CTTGAGCGTA GCGGCAGGAA GGGATTGATG 
CGGATCAATA ACATGGAACA AGTTACTGGG GAATCACCGG CACCACATAC CGCTCTCAAT 
CTGAAAGAGC CTCTATTTGT GGGTGGAGCT CCAGATTTCA GCAAATTAGC CAGGGCAGCG 
GCTGCCTCTA CAAGCTTTGA AGGAGCCATT CAGAAGATTT CCATTAAGGG GCTGCCTGTT 
TTGAGAGAAG AAAACATCCG CAGTGCCATT GAGATCTCTA CTTTCCGCAG CCATCCTTGT 
ACCAAGAAAC CCTTCCCTTG CCAGAATGGG GGGCTTTGTT CTCCTAATAA AGGCAACTAT 
GAGTGTGTTT GCCAGAGGGG TTTCTCAGGC AGCCAGTGTG AAAAAGTGAC CATTGAGAAA 
GCAGCTGGAG AGTCTGAGGC AGTATCATTT GATGGCAAGA CTTACTTGGA ATATCACATT 
GCTGTAACCA AAAGCCAACT AACTAACGAG ATACCTGAAA CTGGTCCTGT GGACAATCCA 
GATGACCCCA GTGAAAAGGC GCTGCTAGTC AACCAATTTG AGCTGAGCAT CAAGACTGAA 
GCCACTCAGG GTCTCATCCT GTGGAGCGGG AAAGGGACAG AACGTGCCTA TTATATTGCC 
TTGGCTGTAG TTGGAGGTTA TGTTGAGATG ACCTATGACC TGGGCTCCAA GCCTGTTGTC 
CTACGATCCA CAGTACCTGT GAACACCAAC GAGTGGATTC ACATCAAAGC TGTACGAGAC 
AACCGAGATG GCATTTTACG AGTTGGCAAT GAGGACCCCG TCCATGGATC CTCTCCTTTT 
GGAGTCAAAG AGCTGGACAC TGATGGAGCC CTATGGCTGG GTGGAATAGA AAAGCTGAGT 
GCCTCATACA AGCTGCCCAA AGCGTACAGC ACGGGATTCA TTGGCTGCAT GAAGGACGTT 
CTGGTGGACC AACAAGACCT GCACTTAGTG GAAGATGCAT TGAATAAACC ACCCATATTG 
TACTGCTCAG CCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951502.1
CDS	678..6995
Translation	MGRRWGAPAVLLWTLWALAAVGLGFSRGPCPERELELREEEANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNKVMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEEHRRLSADKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACASIVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDPCSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRVNPRTRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQDKCKEDCKHNSVCLNRRGVTRCSCDRVTCDSTYKPVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCSFGAACVVKNKKAVCECQQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGAICEAETGRCVCPTECVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKCVNNQCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETVTHANLQTLSPTGTPSPPIVRTLPTPGTTKITKTKKKTDSFQLVLEATSLSPFSSTTMRPVTQTHRAHTSTVRPAITIPTVHPTVLQIYDESGSGSGDEELEASGDQDLSGVEPKEPFDVENEQKEEAEEKRDFVATTVIERASCYNTPLGCCSDGKTPANDAEGTNCPKTKIFQGVLIIEELEGQEVFYTPEMSDYKSELFGETARSIENALDNLLRNSDIKRDFKSIRVRDLGPSNSIRAIVEAHFDPATTMSAMDVQRALLRQLKSSKKRAITIKKPIQENVKFLDFGEIPDWVPLMVTTTTTTAATTSMVATTAVAPTTTTPVVATTTTVARTPLPRRIVAVKTTPVTITQQPVTTPRMTTRRPVTTKRPMTTIPATTRRKPPRIQAVTKKPPRPCESHPCLHGGTCEDNGKDFTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPAPHTALNLKEPLFVGGAPDFSKLARAAAASTSFEGAIQKISIKGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLCSPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSQLTNEIPETGPVDNPDDPSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKELDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDALNKPPILYCSAK

Target ORF information:

RefSeq Version	XM_018096013.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis agrin (agrn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096013.1

ORF Insert Sequence:

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGGTGAGATC CCTGACTGGG TGCCTCTAAT GGTTACAACC 
ACAACTACAA CGGCAGCAAC CACTAGCATG GTAGCTACTA CTGCTGTTGC CCCCACCACC 
ACAACCCCTG TAGTCGCTAC CACAACAACT GTGGCAAGAA CTCCACTACC TAGAAGAATA 
GTTGCAGTCA AGACCACCCC TGTGACAATC ACTCAGCAAC CTGTCACCAC TCCAAGAATG 
ACAACAAGAC GACCAGTAAC TACAAAAAGA CCAATGACCA CTATTCCTGC CACCACCAGG 
AGAAAGCCAC CACGTATTCA AGCAGTCACA AAAAAGCCAC CCCGGCCATG TGAATCACAC 
CCTTGTCTAC ATGGAGGTAC TTGTGAGGAT AATGGGAAGG ATTTCACCTG TAGCTGCCCA 
GCTGGCAGAG GAGGAGCTGT GTGCGAGAAG AGTATTAAGT ATTTTATTCC TGGGTTTGGT 
GGTAAGTCAT ACCTGGCATT CAAGATGATG AAGGCATATC ATACAGTGCG CATAGCCATG 
GAGTTCCGAT CCTCTGAGCA GAACGGGCTT CTCCTATACA ATGGTCAGTC ACTTGGGAAG 
GACTTTATCT CACTTGCAGT TAATAATGGC TTTGTGGAAC TCAGGTTTAA TACTGGATCT 
GGAACTGGAA TAATAACTAG TAAAGTGCCC ATTGAACCTG GCAAGTGGCA TCAGTTAGTA 
GTGAACCGTA ACAGACGCAG TGGCATGTTG TCTGTGGATG GTGAATCCCA TGTCAATGGT 
GAAAGTCCAT CTGGAACCGA TGGCCTCAAC CTAGACACAG ACCTGTTTAT AGGAGGAGCT 
CCTGAGGACC AGATGGCTGT GGTATCAGAA AGGACCTCTT TGTCTGTGGG GCTTAAAGGC 
TGCATCCGAC AACTTGATGT GAACAACCAA CTATACAACC TTCAAGAGGG AGGGGCTGAT 
GTCTTGTATG GAAGTGGGGT GGGAGAGTGT GGAAACAACC CTTGCCAGCC CAACCCATGC 
CATAATGGTG CTGCATGCCA AGTAAAGGAG GCTGAAATGT TCCACTGCCA GTGTCTTAGT 
GGTTATACAG GGCCAACCTG TGCAGATGAA CGGAACCCAT GTGACCCCAA CCCCTGCCAC 
GTTTCTGCTA CCTGTTTGGT TCTACCTGAG GGAGGTGCTA AATGTGAATG TCCAATGGGC 
AGAGAGGGAG AGTTTTGTGA GAAAGTGAGC AACCAGGATC TGAATTTGCC ATTCTTACCT 
CAGTTTAATG GATACTCTTA CTTGGAAATG AAGGGACTAC AGACATTTGC CACTGACCTA 
CAGGACAAGC TTGCCATGGA AGTAGTGTTC CTAGCCAGCA ACCCCAATGG CCTCATCTTC 
TATAATGGGC AGAAAACTGA TGGAAAAGGA GATTTTGTGT CACTGTCCCT TCATGATGGC 
AATCTAGAAT ACCGTTACGA TCTCGGAAAG GGAGCTGCTG TCATCAAGAG TAAGGAAAAG 
ATTCCACTCA ACACATGGCT CAGCGTCTCC CTTGAGCGTA GCGGCAGGAA GGGATTGATG 
CGGATCAATA ACATGGAACA AGTTACTGGG GAATCACCGG CACCACATAC CGCTCTCAAT 
CTGAAAGAGC CTCTATTTGT GGGTGGAGCT CCAGATTTCA GCAAATTAGC CAGGGCAGCG 
GCTGCCTCTA CAAGCTTTGA AGGAGCCATT CAGAAGATTT CCATTAAGGG GCTGCCTGTT 
TTGAGAGAAG AAAACATCCG CAGTGCCATT GAGATCTCTA CTTTCCGCAG CCATCCTTGT 
ACCAAGAAAC CCTTCCCTTG CCAGAATGGG GGGCTTTGTT CTCCTAATAA AGGCAACTAT 
GAGTGTGTTT GCCAGAGGGG TTTCTCAGGC AGCCAGTGTG AAAAAGTGAC CATTGAGAAA 
GCAGCTGGAG AGTCTGAGGC AGTATCATTT GATGGCAAGA CTTACTTGGA ATATCACATT 
GCTGTAACCA AAAGCCAACT AACTAACGAG ATACCTGAAA CTGGTCCTGT GGACAATCCA 
GATGACCCCA GTGAAAAGGC GCTGCTAGTC AACCAATTTG AGCTGAGCAT CAAGACTGAA 
GCCACTCAGG GTCTCATCCT GTGGAGCGGG AAAGGGACAG AACGTGCCTA TTATATTGCC 
TTGGCTGTAG TTGGAGGTTA TGTTGAGATG ACCTATGACC TGGGCTCCAA GCCTGTTGTC 
CTACGATCCA CAGTACCTGT GAACACCAAC GAGTGGATTC ACATCAAAGC TGTACGAGAC 
AACCGAGATG GCATTTTACG AGTTGGCAAT GAGGACCCCG TCCATGGATC CTCTCCTTTT 
GGAGTCAAAG AGCTGGACAC TGATGGAGCC CTATGGCTGG GTGGAATAGA AAAGCTGAGT 
GCCTCATACA AGCTGCCCAA AGCGTACAGC ACGGGATTCA TTGGCTGCAT GAAGGACGTT 
CTGGTGGACC AACAAGACCT GCACTTAGTG GAAGATGCAT TGAATAAACC ACCCATATTG 
TACTGCTCAG CCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agrn ( XM_018096013.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951502.1 Xenopus tropicalis agrin (agrn), transcript variant X2, mRNA.

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CloneID	OXa45876
Clone ID Related Accession (Same CDS sequence)	XM_018096013.1 , XM_018096013.2
Accession Version	XM_018096013.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	agrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096013.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGGTGAGATC CCTGACTGGG TGCCTCTAAT GGTTACAACC 
ACAACTACAA CGGCAGCAAC CACTAGCATG GTAGCTACTA CTGCTGTTGC CCCCACCACC 
ACAACCCCTG TAGTCGCTAC CACAACAACT GTGGCAAGAA CTCCACTACC TAGAAGAATA 
GTTGCAGTCA AGACCACCCC TGTGACAATC ACTCAGCAAC CTGTCACCAC TCCAAGAATG 
ACAACAAGAC GACCAGTAAC TACAAAAAGA CCAATGACCA CTATTCCTGC CACCACCAGG 
AGAAAGCCAC CACGTATTCA AGCAGTCACA AAAAAGCCAC CCCGGCCATG TGAATCACAC 
CCTTGTCTAC ATGGAGGTAC TTGTGAGGAT AATGGGAAGG ATTTCACCTG TAGCTGCCCA 
GCTGGCAGAG GAGGAGCTGT GTGCGAGAAG AGTATTAAGT ATTTTATTCC TGGGTTTGGT 
GGTAAGTCAT ACCTGGCATT CAAGATGATG AAGGCATATC ATACAGTGCG CATAGCCATG 
GAGTTCCGAT CCTCTGAGCA GAACGGGCTT CTCCTATACA ATGGTCAGTC ACTTGGGAAG 
GACTTTATCT CACTTGCAGT TAATAATGGC TTTGTGGAAC TCAGGTTTAA TACTGGATCT 
GGAACTGGAA TAATAACTAG TAAAGTGCCC ATTGAACCTG GCAAGTGGCA TCAGTTAGTA 
GTGAACCGTA ACAGACGCAG TGGCATGTTG TCTGTGGATG GTGAATCCCA TGTCAATGGT 
GAAAGTCCAT CTGGAACCGA TGGCCTCAAC CTAGACACAG ACCTGTTTAT AGGAGGAGCT 
CCTGAGGACC AGATGGCTGT GGTATCAGAA AGGACCTCTT TGTCTGTGGG GCTTAAAGGC 
TGCATCCGAC AACTTGATGT GAACAACCAA CTATACAACC TTCAAGAGGG AGGGGCTGAT 
GTCTTGTATG GAAGTGGGGT GGGAGAGTGT GGAAACAACC CTTGCCAGCC CAACCCATGC 
CATAATGGTG CTGCATGCCA AGTAAAGGAG GCTGAAATGT TCCACTGCCA GTGTCTTAGT 
GGTTATACAG GGCCAACCTG TGCAGATGAA CGGAACCCAT GTGACCCCAA CCCCTGCCAC 
GTTTCTGCTA CCTGTTTGGT TCTACCTGAG GGAGGTGCTA AATGTGAATG TCCAATGGGC 
AGAGAGGGAG AGTTTTGTGA GAAAGTGAGC AACCAGGATC TGAATTTGCC ATTCTTACCT 
CAGTTTAATG GATACTCTTA CTTGGAAATG AAGGGACTAC AGACATTTGC CACTGACCTA 
CAGGACAAGC TTGCCATGGA AGTAGTGTTC CTAGCCAGCA ACCCCAATGG CCTCATCTTC 
TATAATGGGC AGAAAACTGA TGGAAAAGGA GATTTTGTGT CACTGTCCCT TCATGATGGC 
AATCTAGAAT ACCGTTACGA TCTCGGAAAG GGAGCTGCTG TCATCAAGAG TAAGGAAAAG 
ATTCCACTCA ACACATGGCT CAGCGTCTCC CTTGAGCGTA GCGGCAGGAA GGGATTGATG 
CGGATCAATA ACATGGAACA AGTTACTGGG GAATCACCGG CACCACATAC CGCTCTCAAT 
CTGAAAGAGC CTCTATTTGT GGGTGGAGCT CCAGATTTCA GCAAATTAGC CAGGGCAGCG 
GCTGCCTCTA CAAGCTTTGA AGGAGCCATT CAGAAGATTT CCATTAAGGG GCTGCCTGTT 
TTGAGAGAAG AAAACATCCG CAGTGCCATT GAGATCTCTA CTTTCCGCAG CCATCCTTGT 
ACCAAGAAAC CCTTCCCTTG CCAGAATGGG GGGCTTTGTT CTCCTAATAA AGGCAACTAT 
GAGTGTGTTT GCCAGAGGGG TTTCTCAGGC AGCCAGTGTG AAAAAGTGAC CATTGAGAAA 
GCAGCTGGAG AGTCTGAGGC AGTATCATTT GATGGCAAGA CTTACTTGGA ATATCACATT 
GCTGTAACCA AAAGCCAACT AACTAACGAG ATACCTGAAA CTGGTCCTGT GGACAATCCA 
GATGACCCCA GTGAAAAGGC GCTGCTAGTC AACCAATTTG AGCTGAGCAT CAAGACTGAA 
GCCACTCAGG GTCTCATCCT GTGGAGCGGG AAAGGGACAG AACGTGCCTA TTATATTGCC 
TTGGCTGTAG TTGGAGGTTA TGTTGAGATG ACCTATGACC TGGGCTCCAA GCCTGTTGTC 
CTACGATCCA CAGTACCTGT GAACACCAAC GAGTGGATTC ACATCAAAGC TGTACGAGAC 
AACCGAGATG GCATTTTACG AGTTGGCAAT GAGGACCCCG TCCATGGATC CTCTCCTTTT 
GGAGTCAAAG AGCTGGACAC TGATGGAGCC CTATGGCTGG GTGGAATAGA AAAGCTGAGT 
GCCTCATACA AGCTGCCCAA AGCGTACAGC ACGGGATTCA TTGGCTGCAT GAAGGACGTT 
CTGGTGGACC AACAAGACCT GCACTTAGTG GAAGATGCAT TGAATAAACC ACCCATATTG 
TACTGCTCAG CCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951502.1
CDS	303..6620
Translation	MGRRWGAPAVLLWTLWALAAVGLGFSRGPCPERELELREEEANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNKVMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEEHRRLSADKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACASIVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDPCSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRVNPRTRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQDKCKEDCKHNSVCLNRRGVTRCSCDRVTCDSTYKPVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCSFGAACVVKNKKAVCECQQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGAICEAETGRCVCPTECVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKCVNNQCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETVTHANLQTLSPTGTPSPPIVRTLPTPGTTKITKTKKKTDSFQLVLEATSLSPFSSTTMRPVTQTHRAHTSTVRPAITIPTVHPTVLQIYDESGSGSGDEELEASGDQDLSGVEPKEPFDVENEQKEEAEEKRDFVATTVIERASCYNTPLGCCSDGKTPANDAEGTNCPKTKIFQGVLIIEELEGQEVFYTPEMSDYKSELFGETARSIENALDNLLRNSDIKRDFKSIRVRDLGPSNSIRAIVEAHFDPATTMSAMDVQRALLRQLKSSKKRAITIKKPIQENVKFLDFGEIPDWVPLMVTTTTTTAATTSMVATTAVAPTTTTPVVATTTTVARTPLPRRIVAVKTTPVTITQQPVTTPRMTTRRPVTTKRPMTTIPATTRRKPPRIQAVTKKPPRPCESHPCLHGGTCEDNGKDFTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPAPHTALNLKEPLFVGGAPDFSKLARAAAASTSFEGAIQKISIKGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLCSPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSQLTNEIPETGPVDNPDDPSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKELDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDALNKPPILYCSAK

Target ORF information:

RefSeq Version	XM_018096013.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis agrin (agrn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096013.2

ORF Insert Sequence:

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGGTGAGATC CCTGACTGGG TGCCTCTAAT GGTTACAACC 
ACAACTACAA CGGCAGCAAC CACTAGCATG GTAGCTACTA CTGCTGTTGC CCCCACCACC 
ACAACCCCTG TAGTCGCTAC CACAACAACT GTGGCAAGAA CTCCACTACC TAGAAGAATA 
GTTGCAGTCA AGACCACCCC TGTGACAATC ACTCAGCAAC CTGTCACCAC TCCAAGAATG 
ACAACAAGAC GACCAGTAAC TACAAAAAGA CCAATGACCA CTATTCCTGC CACCACCAGG 
AGAAAGCCAC CACGTATTCA AGCAGTCACA AAAAAGCCAC CCCGGCCATG TGAATCACAC 
CCTTGTCTAC ATGGAGGTAC TTGTGAGGAT AATGGGAAGG ATTTCACCTG TAGCTGCCCA 
GCTGGCAGAG GAGGAGCTGT GTGCGAGAAG AGTATTAAGT ATTTTATTCC TGGGTTTGGT 
GGTAAGTCAT ACCTGGCATT CAAGATGATG AAGGCATATC ATACAGTGCG CATAGCCATG 
GAGTTCCGAT CCTCTGAGCA GAACGGGCTT CTCCTATACA ATGGTCAGTC ACTTGGGAAG 
GACTTTATCT CACTTGCAGT TAATAATGGC TTTGTGGAAC TCAGGTTTAA TACTGGATCT 
GGAACTGGAA TAATAACTAG TAAAGTGCCC ATTGAACCTG GCAAGTGGCA TCAGTTAGTA 
GTGAACCGTA ACAGACGCAG TGGCATGTTG TCTGTGGATG GTGAATCCCA TGTCAATGGT 
GAAAGTCCAT CTGGAACCGA TGGCCTCAAC CTAGACACAG ACCTGTTTAT AGGAGGAGCT 
CCTGAGGACC AGATGGCTGT GGTATCAGAA AGGACCTCTT TGTCTGTGGG GCTTAAAGGC 
TGCATCCGAC AACTTGATGT GAACAACCAA CTATACAACC TTCAAGAGGG AGGGGCTGAT 
GTCTTGTATG GAAGTGGGGT GGGAGAGTGT GGAAACAACC CTTGCCAGCC CAACCCATGC 
CATAATGGTG CTGCATGCCA AGTAAAGGAG GCTGAAATGT TCCACTGCCA GTGTCTTAGT 
GGTTATACAG GGCCAACCTG TGCAGATGAA CGGAACCCAT GTGACCCCAA CCCCTGCCAC 
GTTTCTGCTA CCTGTTTGGT TCTACCTGAG GGAGGTGCTA AATGTGAATG TCCAATGGGC 
AGAGAGGGAG AGTTTTGTGA GAAAGTGAGC AACCAGGATC TGAATTTGCC ATTCTTACCT 
CAGTTTAATG GATACTCTTA CTTGGAAATG AAGGGACTAC AGACATTTGC CACTGACCTA 
CAGGACAAGC TTGCCATGGA AGTAGTGTTC CTAGCCAGCA ACCCCAATGG CCTCATCTTC 
TATAATGGGC AGAAAACTGA TGGAAAAGGA GATTTTGTGT CACTGTCCCT TCATGATGGC 
AATCTAGAAT ACCGTTACGA TCTCGGAAAG GGAGCTGCTG TCATCAAGAG TAAGGAAAAG 
ATTCCACTCA ACACATGGCT CAGCGTCTCC CTTGAGCGTA GCGGCAGGAA GGGATTGATG 
CGGATCAATA ACATGGAACA AGTTACTGGG GAATCACCGG CACCACATAC CGCTCTCAAT 
CTGAAAGAGC CTCTATTTGT GGGTGGAGCT CCAGATTTCA GCAAATTAGC CAGGGCAGCG 
GCTGCCTCTA CAAGCTTTGA AGGAGCCATT CAGAAGATTT CCATTAAGGG GCTGCCTGTT 
TTGAGAGAAG AAAACATCCG CAGTGCCATT GAGATCTCTA CTTTCCGCAG CCATCCTTGT 
ACCAAGAAAC CCTTCCCTTG CCAGAATGGG GGGCTTTGTT CTCCTAATAA AGGCAACTAT 
GAGTGTGTTT GCCAGAGGGG TTTCTCAGGC AGCCAGTGTG AAAAAGTGAC CATTGAGAAA 
GCAGCTGGAG AGTCTGAGGC AGTATCATTT GATGGCAAGA CTTACTTGGA ATATCACATT 
GCTGTAACCA AAAGCCAACT AACTAACGAG ATACCTGAAA CTGGTCCTGT GGACAATCCA 
GATGACCCCA GTGAAAAGGC GCTGCTAGTC AACCAATTTG AGCTGAGCAT CAAGACTGAA 
GCCACTCAGG GTCTCATCCT GTGGAGCGGG AAAGGGACAG AACGTGCCTA TTATATTGCC 
TTGGCTGTAG TTGGAGGTTA TGTTGAGATG ACCTATGACC TGGGCTCCAA GCCTGTTGTC 
CTACGATCCA CAGTACCTGT GAACACCAAC GAGTGGATTC ACATCAAAGC TGTACGAGAC 
AACCGAGATG GCATTTTACG AGTTGGCAAT GAGGACCCCG TCCATGGATC CTCTCCTTTT 
GGAGTCAAAG AGCTGGACAC TGATGGAGCC CTATGGCTGG GTGGAATAGA AAAGCTGAGT 
GCCTCATACA AGCTGCCCAA AGCGTACAGC ACGGGATTCA TTGGCTGCAT GAAGGACGTT 
CTGGTGGACC AACAAGACCT GCACTTAGTG GAAGATGCAT TGAATAAACC ACCCATATTG 
TACTGCTCAG CCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agrn ( XM_018096014.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951503.1 Xenopus tropicalis agrin (agrn), transcript variant X3, mRNA.

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CloneID	OXa45877
Clone ID Related Accession (Same CDS sequence)	XM_018096014.1 , XM_018096014.2
Accession Version	XM_018096014.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	agrin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGACTGGGTG CCTCTAATGG TTACAACCAC AACTACAACG 
GCAGCAACCA CTAGCATGGT AGCTACTACT GCTGTTGCCC CCACCACCAC AACCCCTGTA 
GTCGCTACCA CAACAACTGT GGCAAGAACT CCACTACCTA GAAGAATAGT TGCAGTCAAG 
ACCACCCCTG TGACAATCAC TCAGCAACCT GTCACCACTC CAAGAATGAC AACAAGACGA 
CCAGTAACTA CAAAAAGACC AATGACCACT ATTCCTGCCA CCACCAGGAG AAAGCCACCA 
CGTATTCAAG CAGTCACAAA AAAGCCACCC CGGCCATGTG AATCACACCC TTGTCTACAT 
GGAGGTACTT GTGAGGATAA TGGGAAGGAT TTCACCTGTA GCTGCCCAGC TGGCAGAGGA 
GGAGCTGTGT GCGAGAAGAG TATTAAGTAT TTTATTCCTG GGTTTGGTGG TAAGTCATAC 
CTGGCATTCA AGATGATGAA GGCATATCAT ACAGTGCGCA TAGCCATGGA GTTCCGATCC 
TCTGAGCAGA ACGGGCTTCT CCTATACAAT GGTCAGTCAC TTGGGAAGGA CTTTATCTCA 
CTTGCAGTTA ATAATGGCTT TGTGGAACTC AGGTTTAATA CTGGATCTGG AACTGGAATA 
ATAACTAGTA AAGTGCCCAT TGAACCTGGC AAGTGGCATC AGTTAGTAGT GAACCGTAAC 
AGACGCAGTG GCATGTTGTC TGTGGATGGT GAATCCCATG TCAATGGTGA AAGTCCATCT 
GGAACCGATG GCCTCAACCT AGACACAGAC CTGTTTATAG GAGGAGCTCC TGAGGACCAG 
ATGGCTGTGG TATCAGAAAG GACCTCTTTG TCTGTGGGGC TTAAAGGCTG CATCCGACAA 
CTTGATGTGA ACAACCAACT ATACAACCTT CAAGAGGGAG GGGCTGATGT CTTGTATGGA 
AGTGGGGTGG GAGAGTGTGG AAACAACCCT TGCCAGCCCA ACCCATGCCA TAATGGTGCT 
GCATGCCAAG TAAAGGAGGC TGAAATGTTC CACTGCCAGT GTCTTAGTGG TTATACAGGG 
CCAACCTGTG CAGATGAACG GAACCCATGT GACCCCAACC CCTGCCACGT TTCTGCTACC 
TGTTTGGTTC TACCTGAGGG AGGTGCTAAA TGTGAATGTC CAATGGGCAG AGAGGGAGAG 
TTTTGTGAGA AAGTGAGCAA CCAGGATCTG AATTTGCCAT TCTTACCTCA GTTTAATGGA 
TACTCTTACT TGGAAATGAA GGGACTACAG ACATTTGCCA CTGACCTACA GGACAAGCTT 
GCCATGGAAG TAGTGTTCCT AGCCAGCAAC CCCAATGGCC TCATCTTCTA TAATGGGCAG 
AAAACTGATG GAAAAGGAGA TTTTGTGTCA CTGTCCCTTC ATGATGGCAA TCTAGAATAC 
CGTTACGATC TCGGAAAGGG AGCTGCTGTC ATCAAGAGTA AGGAAAAGAT TCCACTCAAC 
ACATGGCTCA GCGTCTCCCT TGAGCGTAGC GGCAGGAAGG GATTGATGCG GATCAATAAC 
ATGGAACAAG TTACTGGGGA ATCACCGAAA TCCCGTAAGG CACCACATAC CGCTCTCAAT 
CTGAAAGAGC CTCTATTTGT GGGTGGAGCT CCAGATTTCA GCAAATTAGC CAGGGCAGCG 
GCTGCCTCTA CAAGCTTTGA AGGAGCCATT CAGAAGATTT CCATTAAGGG GCTGCCTGTT 
TTGAGAGAAG AAAACATCCG CAGTGCCATT GAGATCTCTA CTTTCCGCAG CCATCCTTGT 
ACCAAGAAAC CCTTCCCTTG CCAGAATGGG GGGCTTTGTT CTCCTAATAA AGGCAACTAT 
GAGTGTGTTT GCCAGAGGGG TTTCTCAGGC AGCCAGTGTG AAAAAGTGAC CATTGAGAAA 
GCAGCTGGAG AGTCTGAGGC AGTATCATTT GATGGCAAGA CTTACTTGGA ATATCACATT 
GCTGTAACCA AAAGCCAACT AACTAACGAG ATACCTGAAA CTGGTCCTGT GGACAATCCA 
GATGACCCCA GTGAAAAGGC GCTGCTAGTC AACCAATTTG AGCTGAGCAT CAAGACTGAA 
GCCACTCAGG GTCTCATCCT GTGGAGCGGG AAAGGGACAG AACGTGCCTA TTATATTGCC 
TTGGCTGTAG TTGGAGGTTA TGTTGAGATG ACCTATGACC TGGGCTCCAA GCCTGTTGTC 
CTACGATCCA CAGTACCTGT GAACACCAAC GAGTGGATTC ACATCAAAGC TGTACGAGAC 
AACCGAGATG GCATTTTACG AGTTGGCAAT GAGGACCCCG TCCATGGATC CTCTCCTTTT 
GGAGTCAAAG AGCTGGACAC TGATGGAGCC CTATGGCTGG GTGGAATAGA AAAGCTGAGT 
GCCTCATACA AGCTGCCCAA AGCGTACAGC ACGGGATTCA TTGGCTGCAT GAAGGACGTT 
CTGGTGGACC AACAAGACCT GCACTTAGTG GAAGATGCAT TGAATAAACC ACCCATATTG 
TACTGCTCAG CCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951503.1
CDS	678..6995
Translation	MGRRWGAPAVLLWTLWALAAVGLGFSRGPCPERELELREEEANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNKVMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEEHRRLSADKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACASIVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDPCSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRVNPRTRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQDKCKEDCKHNSVCLNRRGVTRCSCDRVTCDSTYKPVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCSFGAACVVKNKKAVCECQQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGAICEAETGRCVCPTECVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKCVNNQCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETVTHANLQTLSPTGTPSPPIVRTLPTPGTTKITKTKKKTDSFQLVLEATSLSPFSSTTMRPVTQTHRAHTSTVRPAITIPTVHPTVLQIYDESGSGSGDEELEASGDQDLSGVEPKEPFDVENEQKEEAEEKRDFVATTVIERASCYNTPLGCCSDGKTPANDAEGTNCPKTKIFQGVLIIEELEGQEVFYTPEMSDYKSELFGETARSIENALDNLLRNSDIKRDFKSIRVRDLGPSNSIRAIVEAHFDPATTMSAMDVQRALLRQLKSSKKRAITIKKPIQENVKFLDFDWVPLMVTTTTTTAATTSMVATTAVAPTTTTPVVATTTTVARTPLPRRIVAVKTTPVTITQQPVTTPRMTTRRPVTTKRPMTTIPATTRRKPPRIQAVTKKPPRPCESHPCLHGGTCEDNGKDFTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNLKEPLFVGGAPDFSKLARAAAASTSFEGAIQKISIKGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLCSPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSQLTNEIPETGPVDNPDDPSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKELDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDALNKPPILYCSAK

Target ORF information:

RefSeq Version	XM_018096014.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis agrin (agrn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096014.1

ORF Insert Sequence:

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGACTGGGTG CCTCTAATGG TTACAACCAC AACTACAACG 
GCAGCAACCA CTAGCATGGT AGCTACTACT GCTGTTGCCC CCACCACCAC AACCCCTGTA 
GTCGCTACCA CAACAACTGT GGCAAGAACT CCACTACCTA GAAGAATAGT TGCAGTCAAG 
ACCACCCCTG TGACAATCAC TCAGCAACCT GTCACCACTC CAAGAATGAC AACAAGACGA 
CCAGTAACTA CAAAAAGACC AATGACCACT ATTCCTGCCA CCACCAGGAG AAAGCCACCA 
CGTATTCAAG CAGTCACAAA AAAGCCACCC CGGCCATGTG AATCACACCC TTGTCTACAT 
GGAGGTACTT GTGAGGATAA TGGGAAGGAT TTCACCTGTA GCTGCCCAGC TGGCAGAGGA 
GGAGCTGTGT GCGAGAAGAG TATTAAGTAT TTTATTCCTG GGTTTGGTGG TAAGTCATAC 
CTGGCATTCA AGATGATGAA GGCATATCAT ACAGTGCGCA TAGCCATGGA GTTCCGATCC 
TCTGAGCAGA ACGGGCTTCT CCTATACAAT GGTCAGTCAC TTGGGAAGGA CTTTATCTCA 
CTTGCAGTTA ATAATGGCTT TGTGGAACTC AGGTTTAATA CTGGATCTGG AACTGGAATA 
ATAACTAGTA AAGTGCCCAT TGAACCTGGC AAGTGGCATC AGTTAGTAGT GAACCGTAAC 
AGACGCAGTG GCATGTTGTC TGTGGATGGT GAATCCCATG TCAATGGTGA AAGTCCATCT 
GGAACCGATG GCCTCAACCT AGACACAGAC CTGTTTATAG GAGGAGCTCC TGAGGACCAG 
ATGGCTGTGG TATCAGAAAG GACCTCTTTG TCTGTGGGGC TTAAAGGCTG CATCCGACAA 
CTTGATGTGA ACAACCAACT ATACAACCTT CAAGAGGGAG GGGCTGATGT CTTGTATGGA 
AGTGGGGTGG GAGAGTGTGG AAACAACCCT TGCCAGCCCA ACCCATGCCA TAATGGTGCT 
GCATGCCAAG TAAAGGAGGC TGAAATGTTC CACTGCCAGT GTCTTAGTGG TTATACAGGG 
CCAACCTGTG CAGATGAACG GAACCCATGT GACCCCAACC CCTGCCACGT TTCTGCTACC 
TGTTTGGTTC TACCTGAGGG AGGTGCTAAA TGTGAATGTC CAATGGGCAG AGAGGGAGAG 
TTTTGTGAGA AAGTGAGCAA CCAGGATCTG AATTTGCCAT TCTTACCTCA GTTTAATGGA 
TACTCTTACT TGGAAATGAA GGGACTACAG ACATTTGCCA CTGACCTACA GGACAAGCTT 
GCCATGGAAG TAGTGTTCCT AGCCAGCAAC CCCAATGGCC TCATCTTCTA TAATGGGCAG 
AAAACTGATG GAAAAGGAGA TTTTGTGTCA CTGTCCCTTC ATGATGGCAA TCTAGAATAC 
CGTTACGATC TCGGAAAGGG AGCTGCTGTC ATCAAGAGTA AGGAAAAGAT TCCACTCAAC 
ACATGGCTCA GCGTCTCCCT TGAGCGTAGC GGCAGGAAGG GATTGATGCG GATCAATAAC 
ATGGAACAAG TTACTGGGGA ATCACCGAAA TCCCGTAAGG CACCACATAC CGCTCTCAAT 
CTGAAAGAGC CTCTATTTGT GGGTGGAGCT CCAGATTTCA GCAAATTAGC CAGGGCAGCG 
GCTGCCTCTA CAAGCTTTGA AGGAGCCATT CAGAAGATTT CCATTAAGGG GCTGCCTGTT 
TTGAGAGAAG AAAACATCCG CAGTGCCATT GAGATCTCTA CTTTCCGCAG CCATCCTTGT 
ACCAAGAAAC CCTTCCCTTG CCAGAATGGG GGGCTTTGTT CTCCTAATAA AGGCAACTAT 
GAGTGTGTTT GCCAGAGGGG TTTCTCAGGC AGCCAGTGTG AAAAAGTGAC CATTGAGAAA 
GCAGCTGGAG AGTCTGAGGC AGTATCATTT GATGGCAAGA CTTACTTGGA ATATCACATT 
GCTGTAACCA AAAGCCAACT AACTAACGAG ATACCTGAAA CTGGTCCTGT GGACAATCCA 
GATGACCCCA GTGAAAAGGC GCTGCTAGTC AACCAATTTG AGCTGAGCAT CAAGACTGAA 
GCCACTCAGG GTCTCATCCT GTGGAGCGGG AAAGGGACAG AACGTGCCTA TTATATTGCC 
TTGGCTGTAG TTGGAGGTTA TGTTGAGATG ACCTATGACC TGGGCTCCAA GCCTGTTGTC 
CTACGATCCA CAGTACCTGT GAACACCAAC GAGTGGATTC ACATCAAAGC TGTACGAGAC 
AACCGAGATG GCATTTTACG AGTTGGCAAT GAGGACCCCG TCCATGGATC CTCTCCTTTT 
GGAGTCAAAG AGCTGGACAC TGATGGAGCC CTATGGCTGG GTGGAATAGA AAAGCTGAGT 
GCCTCATACA AGCTGCCCAA AGCGTACAGC ACGGGATTCA TTGGCTGCAT GAAGGACGTT 
CTGGTGGACC AACAAGACCT GCACTTAGTG GAAGATGCAT TGAATAAACC ACCCATATTG 
TACTGCTCAG CCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agrn ( XM_018096014.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951503.1 Xenopus tropicalis agrin (agrn), transcript variant X3, mRNA.

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CloneID	OXa45877
Clone ID Related Accession (Same CDS sequence)	XM_018096014.1 , XM_018096014.2
Accession Version	XM_018096014.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	agrin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGACTGGGTG CCTCTAATGG TTACAACCAC AACTACAACG 
GCAGCAACCA CTAGCATGGT AGCTACTACT GCTGTTGCCC CCACCACCAC AACCCCTGTA 
GTCGCTACCA CAACAACTGT GGCAAGAACT CCACTACCTA GAAGAATAGT TGCAGTCAAG 
ACCACCCCTG TGACAATCAC TCAGCAACCT GTCACCACTC CAAGAATGAC AACAAGACGA 
CCAGTAACTA CAAAAAGACC AATGACCACT ATTCCTGCCA CCACCAGGAG AAAGCCACCA 
CGTATTCAAG CAGTCACAAA AAAGCCACCC CGGCCATGTG AATCACACCC TTGTCTACAT 
GGAGGTACTT GTGAGGATAA TGGGAAGGAT TTCACCTGTA GCTGCCCAGC TGGCAGAGGA 
GGAGCTGTGT GCGAGAAGAG TATTAAGTAT TTTATTCCTG GGTTTGGTGG TAAGTCATAC 
CTGGCATTCA AGATGATGAA GGCATATCAT ACAGTGCGCA TAGCCATGGA GTTCCGATCC 
TCTGAGCAGA ACGGGCTTCT CCTATACAAT GGTCAGTCAC TTGGGAAGGA CTTTATCTCA 
CTTGCAGTTA ATAATGGCTT TGTGGAACTC AGGTTTAATA CTGGATCTGG AACTGGAATA 
ATAACTAGTA AAGTGCCCAT TGAACCTGGC AAGTGGCATC AGTTAGTAGT GAACCGTAAC 
AGACGCAGTG GCATGTTGTC TGTGGATGGT GAATCCCATG TCAATGGTGA AAGTCCATCT 
GGAACCGATG GCCTCAACCT AGACACAGAC CTGTTTATAG GAGGAGCTCC TGAGGACCAG 
ATGGCTGTGG TATCAGAAAG GACCTCTTTG TCTGTGGGGC TTAAAGGCTG CATCCGACAA 
CTTGATGTGA ACAACCAACT ATACAACCTT CAAGAGGGAG GGGCTGATGT CTTGTATGGA 
AGTGGGGTGG GAGAGTGTGG AAACAACCCT TGCCAGCCCA ACCCATGCCA TAATGGTGCT 
GCATGCCAAG TAAAGGAGGC TGAAATGTTC CACTGCCAGT GTCTTAGTGG TTATACAGGG 
CCAACCTGTG CAGATGAACG GAACCCATGT GACCCCAACC CCTGCCACGT TTCTGCTACC 
TGTTTGGTTC TACCTGAGGG AGGTGCTAAA TGTGAATGTC CAATGGGCAG AGAGGGAGAG 
TTTTGTGAGA AAGTGAGCAA CCAGGATCTG AATTTGCCAT TCTTACCTCA GTTTAATGGA 
TACTCTTACT TGGAAATGAA GGGACTACAG ACATTTGCCA CTGACCTACA GGACAAGCTT 
GCCATGGAAG TAGTGTTCCT AGCCAGCAAC CCCAATGGCC TCATCTTCTA TAATGGGCAG 
AAAACTGATG GAAAAGGAGA TTTTGTGTCA CTGTCCCTTC ATGATGGCAA TCTAGAATAC 
CGTTACGATC TCGGAAAGGG AGCTGCTGTC ATCAAGAGTA AGGAAAAGAT TCCACTCAAC 
ACATGGCTCA GCGTCTCCCT TGAGCGTAGC GGCAGGAAGG GATTGATGCG GATCAATAAC 
ATGGAACAAG TTACTGGGGA ATCACCGAAA TCCCGTAAGG CACCACATAC CGCTCTCAAT 
CTGAAAGAGC CTCTATTTGT GGGTGGAGCT CCAGATTTCA GCAAATTAGC CAGGGCAGCG 
GCTGCCTCTA CAAGCTTTGA AGGAGCCATT CAGAAGATTT CCATTAAGGG GCTGCCTGTT 
TTGAGAGAAG AAAACATCCG CAGTGCCATT GAGATCTCTA CTTTCCGCAG CCATCCTTGT 
ACCAAGAAAC CCTTCCCTTG CCAGAATGGG GGGCTTTGTT CTCCTAATAA AGGCAACTAT 
GAGTGTGTTT GCCAGAGGGG TTTCTCAGGC AGCCAGTGTG AAAAAGTGAC CATTGAGAAA 
GCAGCTGGAG AGTCTGAGGC AGTATCATTT GATGGCAAGA CTTACTTGGA ATATCACATT 
GCTGTAACCA AAAGCCAACT AACTAACGAG ATACCTGAAA CTGGTCCTGT GGACAATCCA 
GATGACCCCA GTGAAAAGGC GCTGCTAGTC AACCAATTTG AGCTGAGCAT CAAGACTGAA 
GCCACTCAGG GTCTCATCCT GTGGAGCGGG AAAGGGACAG AACGTGCCTA TTATATTGCC 
TTGGCTGTAG TTGGAGGTTA TGTTGAGATG ACCTATGACC TGGGCTCCAA GCCTGTTGTC 
CTACGATCCA CAGTACCTGT GAACACCAAC GAGTGGATTC ACATCAAAGC TGTACGAGAC 
AACCGAGATG GCATTTTACG AGTTGGCAAT GAGGACCCCG TCCATGGATC CTCTCCTTTT 
GGAGTCAAAG AGCTGGACAC TGATGGAGCC CTATGGCTGG GTGGAATAGA AAAGCTGAGT 
GCCTCATACA AGCTGCCCAA AGCGTACAGC ACGGGATTCA TTGGCTGCAT GAAGGACGTT 
CTGGTGGACC AACAAGACCT GCACTTAGTG GAAGATGCAT TGAATAAACC ACCCATATTG 
TACTGCTCAG CCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951503.1
CDS	303..6620
Translation	MGRRWGAPAVLLWTLWALAAVGLGFSRGPCPERELELREEEANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNKVMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEEHRRLSADKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACASIVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDPCSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRVNPRTRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQDKCKEDCKHNSVCLNRRGVTRCSCDRVTCDSTYKPVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCSFGAACVVKNKKAVCECQQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGAICEAETGRCVCPTECVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKCVNNQCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETVTHANLQTLSPTGTPSPPIVRTLPTPGTTKITKTKKKTDSFQLVLEATSLSPFSSTTMRPVTQTHRAHTSTVRPAITIPTVHPTVLQIYDESGSGSGDEELEASGDQDLSGVEPKEPFDVENEQKEEAEEKRDFVATTVIERASCYNTPLGCCSDGKTPANDAEGTNCPKTKIFQGVLIIEELEGQEVFYTPEMSDYKSELFGETARSIENALDNLLRNSDIKRDFKSIRVRDLGPSNSIRAIVEAHFDPATTMSAMDVQRALLRQLKSSKKRAITIKKPIQENVKFLDFDWVPLMVTTTTTTAATTSMVATTAVAPTTTTPVVATTTTVARTPLPRRIVAVKTTPVTITQQPVTTPRMTTRRPVTTKRPMTTIPATTRRKPPRIQAVTKKPPRPCESHPCLHGGTCEDNGKDFTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNLKEPLFVGGAPDFSKLARAAAASTSFEGAIQKISIKGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLCSPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSQLTNEIPETGPVDNPDDPSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKELDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDALNKPPILYCSAK

Target ORF information:

RefSeq Version	XM_018096014.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis agrin (agrn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096014.2

ORF Insert Sequence:

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGACTGGGTG CCTCTAATGG TTACAACCAC AACTACAACG 
GCAGCAACCA CTAGCATGGT AGCTACTACT GCTGTTGCCC CCACCACCAC AACCCCTGTA 
GTCGCTACCA CAACAACTGT GGCAAGAACT CCACTACCTA GAAGAATAGT TGCAGTCAAG 
ACCACCCCTG TGACAATCAC TCAGCAACCT GTCACCACTC CAAGAATGAC AACAAGACGA 
CCAGTAACTA CAAAAAGACC AATGACCACT ATTCCTGCCA CCACCAGGAG AAAGCCACCA 
CGTATTCAAG CAGTCACAAA AAAGCCACCC CGGCCATGTG AATCACACCC TTGTCTACAT 
GGAGGTACTT GTGAGGATAA TGGGAAGGAT TTCACCTGTA GCTGCCCAGC TGGCAGAGGA 
GGAGCTGTGT GCGAGAAGAG TATTAAGTAT TTTATTCCTG GGTTTGGTGG TAAGTCATAC 
CTGGCATTCA AGATGATGAA GGCATATCAT ACAGTGCGCA TAGCCATGGA GTTCCGATCC 
TCTGAGCAGA ACGGGCTTCT CCTATACAAT GGTCAGTCAC TTGGGAAGGA CTTTATCTCA 
CTTGCAGTTA ATAATGGCTT TGTGGAACTC AGGTTTAATA CTGGATCTGG AACTGGAATA 
ATAACTAGTA AAGTGCCCAT TGAACCTGGC AAGTGGCATC AGTTAGTAGT GAACCGTAAC 
AGACGCAGTG GCATGTTGTC TGTGGATGGT GAATCCCATG TCAATGGTGA AAGTCCATCT 
GGAACCGATG GCCTCAACCT AGACACAGAC CTGTTTATAG GAGGAGCTCC TGAGGACCAG 
ATGGCTGTGG TATCAGAAAG GACCTCTTTG TCTGTGGGGC TTAAAGGCTG CATCCGACAA 
CTTGATGTGA ACAACCAACT ATACAACCTT CAAGAGGGAG GGGCTGATGT CTTGTATGGA 
AGTGGGGTGG GAGAGTGTGG AAACAACCCT TGCCAGCCCA ACCCATGCCA TAATGGTGCT 
GCATGCCAAG TAAAGGAGGC TGAAATGTTC CACTGCCAGT GTCTTAGTGG TTATACAGGG 
CCAACCTGTG CAGATGAACG GAACCCATGT GACCCCAACC CCTGCCACGT TTCTGCTACC 
TGTTTGGTTC TACCTGAGGG AGGTGCTAAA TGTGAATGTC CAATGGGCAG AGAGGGAGAG 
TTTTGTGAGA AAGTGAGCAA CCAGGATCTG AATTTGCCAT TCTTACCTCA GTTTAATGGA 
TACTCTTACT TGGAAATGAA GGGACTACAG ACATTTGCCA CTGACCTACA GGACAAGCTT 
GCCATGGAAG TAGTGTTCCT AGCCAGCAAC CCCAATGGCC TCATCTTCTA TAATGGGCAG 
AAAACTGATG GAAAAGGAGA TTTTGTGTCA CTGTCCCTTC ATGATGGCAA TCTAGAATAC 
CGTTACGATC TCGGAAAGGG AGCTGCTGTC ATCAAGAGTA AGGAAAAGAT TCCACTCAAC 
ACATGGCTCA GCGTCTCCCT TGAGCGTAGC GGCAGGAAGG GATTGATGCG GATCAATAAC 
ATGGAACAAG TTACTGGGGA ATCACCGAAA TCCCGTAAGG CACCACATAC CGCTCTCAAT 
CTGAAAGAGC CTCTATTTGT GGGTGGAGCT CCAGATTTCA GCAAATTAGC CAGGGCAGCG 
GCTGCCTCTA CAAGCTTTGA AGGAGCCATT CAGAAGATTT CCATTAAGGG GCTGCCTGTT 
TTGAGAGAAG AAAACATCCG CAGTGCCATT GAGATCTCTA CTTTCCGCAG CCATCCTTGT 
ACCAAGAAAC CCTTCCCTTG CCAGAATGGG GGGCTTTGTT CTCCTAATAA AGGCAACTAT 
GAGTGTGTTT GCCAGAGGGG TTTCTCAGGC AGCCAGTGTG AAAAAGTGAC CATTGAGAAA 
GCAGCTGGAG AGTCTGAGGC AGTATCATTT GATGGCAAGA CTTACTTGGA ATATCACATT 
GCTGTAACCA AAAGCCAACT AACTAACGAG ATACCTGAAA CTGGTCCTGT GGACAATCCA 
GATGACCCCA GTGAAAAGGC GCTGCTAGTC AACCAATTTG AGCTGAGCAT CAAGACTGAA 
GCCACTCAGG GTCTCATCCT GTGGAGCGGG AAAGGGACAG AACGTGCCTA TTATATTGCC 
TTGGCTGTAG TTGGAGGTTA TGTTGAGATG ACCTATGACC TGGGCTCCAA GCCTGTTGTC 
CTACGATCCA CAGTACCTGT GAACACCAAC GAGTGGATTC ACATCAAAGC TGTACGAGAC 
AACCGAGATG GCATTTTACG AGTTGGCAAT GAGGACCCCG TCCATGGATC CTCTCCTTTT 
GGAGTCAAAG AGCTGGACAC TGATGGAGCC CTATGGCTGG GTGGAATAGA AAAGCTGAGT 
GCCTCATACA AGCTGCCCAA AGCGTACAGC ACGGGATTCA TTGGCTGCAT GAAGGACGTT 
CTGGTGGACC AACAAGACCT GCACTTAGTG GAAGATGCAT TGAATAAACC ACCCATATTG 
TACTGCTCAG CCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agrn ( XM_018096015.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951504.1 Xenopus tropicalis agrin (agrn), transcript variant X5, mRNA.

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CloneID	OXa45878
Clone ID Related Accession (Same CDS sequence)	XM_018096015.1 , XM_018096015.2
Accession Version	XM_018096015.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	agrin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGGTGAGATC CCTGACTGGG TGCCTCTAAT GGTTACAACC 
ACAACTACAA CGGCAGCAAC CACTAGCATG GTAGCTACTA CTGCTGTTGC CCCCACCACC 
ACAACCCCTG TAGTCGCTAC CACAACAACT GTGGCAAGAA CTCCACTACC TAGAAGAATA 
GTTGCAGTCA AGACCACCCC TGTGACAATC ACTCAGCAAC CTGTCACCAC TCCAAGAATG 
ACAACAAGAC GACCAGTAAC TACAAAAAGA CCAATGACCA CTATTCCTGC CACCACCAGG 
AGAAAGCCAC CACGTATTCA AGCAGTCACA AAAAAGCCAC CCCGGCCATG TGAATCACAC 
CCTTGTCTAC ATGGAGGTAC TTGTGAGGAT AATGGGAAGG ATTTCACCTG TAGCTGCCCA 
GCTGGCAGAG GAGGAGCTGT GTGCGAGAAG AGTATTAAGT ATTTTATTCC TGGGTTTGGT 
GGTAAGTCAT ACCTGGCATT CAAGATGATG AAGGCATATC ATACAGTGCG CATAGCCATG 
GAGTTCCGAT CCTCTGAGCA GAACGGGCTT CTCCTATACA ATGGTCAGTC ACTTGGGAAG 
GACTTTATCT CACTTGCAGT TAATAATGGC TTTGTGGAAC TCAGGTTTAA TACTGGATCT 
GGAACTGGAA TAATAACTAG TAAAGTGCCC ATTGAACCTG GCAAGTGGCA TCAGTTAGTA 
GTGAACCGTA ACAGACGCAG TGGCATGTTG TCTGTGGATG GTGAATCCCA TGTCAATGGT 
GAAAGTCCAT CTGGAACCGA TGGCCTCAAC CTAGACACAG ACCTGTTTAT AGGAGGAGCT 
CCTGAGGACC AGATGGCTGT GGTATCAGAA AGGACCTCTT TGTCTGTGGG GCTTAAAGGC 
TGCATCCGAC AACTTGATGT GAACAACCAA CTATACAACC TTCAAGAGGG AGGGGCTGAT 
GTCTTGTATG GAAGTGGGGT GGGAGAGTGT GGAAACAACC CTTGCCAGCC CAACCCATGC 
CATAATGGTG CTGCATGCCA AGTAAAGGAG GCTGAAATGT TCCACTGCCA GTGTCTTAGT 
GGTTATACAG GGCCAACCTG TGCAGATGAA CGGAACCCAT GTGACCCCAA CCCCTGCCAC 
GTTTCTGCTA CCTGTTTGGT TCTACCTGAG GGAGGTGCTA AATGTGAATG TCCAATGGGC 
AGAGAGGGAG AGTTTTGTGA GAAAGTGAGC AACCAGGATC TGAATTTGCC ATTCTTACCT 
CAGTTTAATG GATACTCTTA CTTGGAAATG AAGGGACTAC AGACATTTGC CACTGACCTA 
CAGGACAAGC TTGCCATGGA AGTAGTGTTC CTAGCCAGCA ACCCCAATGG CCTCATCTTC 
TATAATGGGC AGAAAACTGA TGGAAAAGGA GATTTTGTGT CACTGTCCCT TCATGATGGC 
AATCTAGAAT ACCGTTACGA TCTCGGAAAG GGAGCTGCTG TCATCAAGAG TAAGGAAAAG 
ATTCCACTCA ACACATGGCT CAGCGTCTCC CTTGAGCGTA GCGGCAGGAA GGGATTGATG 
CGGATCAATA ACATGGAACA AGTTACTGGG GAATCACCGA AATCCCGTAA GGCACCACAT 
ACCGCTCTCA ATCTGAAAGA GCCTCTATTT GTGGGTGGAG CTCCAGATTT CAGCAAATTA 
GCCAGGGCAG CGGCTGCCTC TACAAGCTTT GAAGGAGCCA TTCAGAAGAT TTCCATTAAG 
GGGCTGCCTG TTTTGAGAGA AGAAAACATC CGCAGTGCCA TTGAGATCTC TACTTTCCGC 
AGCCATCCTT GTACCAAGAA ACCCTTCCCT TGCCAGAATG GGGGGCTTTG TTCTCCTAAT 
AAAGGCAACT ATGAGTGTGT TTGCCAGAGG GGTTTCTCAG GCAGCCAGTG TGAAAAAGTG 
ACCATTGAGA AAGCAGCTGG AGAGTCTGAG GCAGTATCAT TTGATGGCAA GACTTACTTG 
GAATATCACA TTGCTGTAAC CAAAAGAACT GGTCCTGTGG ACAATCCAGA TGACCCCAGT 
GAAAAGGCGC TGCTAGTCAA CCAATTTGAG CTGAGCATCA AGACTGAAGC CACTCAGGGT 
CTCATCCTGT GGAGCGGGAA AGGGACAGAA CGTGCCTATT ATATTGCCTT GGCTGTAGTT 
GGAGGTTATG TTGAGATGAC CTATGACCTG GGCTCCAAGC CTGTTGTCCT ACGATCCACA 
GTACCTGTGA ACACCAACGA GTGGATTCAC ATCAAAGCTG TACGAGACAA CCGAGATGGC 
ATTTTACGAG TTGGCAATGA GGACCCCGTC CATGGATCCT CTCCTTTTGG AGTCAAAGAG 
CTGGACACTG ATGGAGCCCT ATGGCTGGGT GGAATAGAAA AGCTGAGTGC CTCATACAAG 
CTGCCCAAAG CGTACAGCAC GGGATTCATT GGCTGCATGA AGGACGTTCT GGTGGACCAA 
CAAGACCTGC ACTTAGTGGA AGATGCATTG AATAAACCAC CCATATTGTA CTGCTCAGCC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951504.1
CDS	679..6984
Translation	MGRRWGAPAVLLWTLWALAAVGLGFSRGPCPERELELREEEANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNKVMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEEHRRLSADKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACASIVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDPCSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRVNPRTRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQDKCKEDCKHNSVCLNRRGVTRCSCDRVTCDSTYKPVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCSFGAACVVKNKKAVCECQQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGAICEAETGRCVCPTECVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKCVNNQCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETVTHANLQTLSPTGTPSPPIVRTLPTPGTTKITKTKKKTDSFQLVLEATSLSPFSSTTMRPVTQTHRAHTSTVRPAITIPTVHPTVLQIYDESGSGSGDEELEASGDQDLSGVEPKEPFDVENEQKEEAEEKRDFVATTVIERASCYNTPLGCCSDGKTPANDAEGTNCPKTKIFQGVLIIEELEGQEVFYTPEMSDYKSELFGETARSIENALDNLLRNSDIKRDFKSIRVRDLGPSNSIRAIVEAHFDPATTMSAMDVQRALLRQLKSSKKRAITIKKPIQENVKFLDFGEIPDWVPLMVTTTTTTAATTSMVATTAVAPTTTTPVVATTTTVARTPLPRRIVAVKTTPVTITQQPVTTPRMTTRRPVTTKRPMTTIPATTRRKPPRIQAVTKKPPRPCESHPCLHGGTCEDNGKDFTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNLKEPLFVGGAPDFSKLARAAAASTSFEGAIQKISIKGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLCSPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKRTGPVDNPDDPSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKELDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDALNKPPILYCSAK

Target ORF information:

RefSeq Version	XM_018096015.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis agrin (agrn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096015.1

ORF Insert Sequence:

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGGTGAGATC CCTGACTGGG TGCCTCTAAT GGTTACAACC 
ACAACTACAA CGGCAGCAAC CACTAGCATG GTAGCTACTA CTGCTGTTGC CCCCACCACC 
ACAACCCCTG TAGTCGCTAC CACAACAACT GTGGCAAGAA CTCCACTACC TAGAAGAATA 
GTTGCAGTCA AGACCACCCC TGTGACAATC ACTCAGCAAC CTGTCACCAC TCCAAGAATG 
ACAACAAGAC GACCAGTAAC TACAAAAAGA CCAATGACCA CTATTCCTGC CACCACCAGG 
AGAAAGCCAC CACGTATTCA AGCAGTCACA AAAAAGCCAC CCCGGCCATG TGAATCACAC 
CCTTGTCTAC ATGGAGGTAC TTGTGAGGAT AATGGGAAGG ATTTCACCTG TAGCTGCCCA 
GCTGGCAGAG GAGGAGCTGT GTGCGAGAAG AGTATTAAGT ATTTTATTCC TGGGTTTGGT 
GGTAAGTCAT ACCTGGCATT CAAGATGATG AAGGCATATC ATACAGTGCG CATAGCCATG 
GAGTTCCGAT CCTCTGAGCA GAACGGGCTT CTCCTATACA ATGGTCAGTC ACTTGGGAAG 
GACTTTATCT CACTTGCAGT TAATAATGGC TTTGTGGAAC TCAGGTTTAA TACTGGATCT 
GGAACTGGAA TAATAACTAG TAAAGTGCCC ATTGAACCTG GCAAGTGGCA TCAGTTAGTA 
GTGAACCGTA ACAGACGCAG TGGCATGTTG TCTGTGGATG GTGAATCCCA TGTCAATGGT 
GAAAGTCCAT CTGGAACCGA TGGCCTCAAC CTAGACACAG ACCTGTTTAT AGGAGGAGCT 
CCTGAGGACC AGATGGCTGT GGTATCAGAA AGGACCTCTT TGTCTGTGGG GCTTAAAGGC 
TGCATCCGAC AACTTGATGT GAACAACCAA CTATACAACC TTCAAGAGGG AGGGGCTGAT 
GTCTTGTATG GAAGTGGGGT GGGAGAGTGT GGAAACAACC CTTGCCAGCC CAACCCATGC 
CATAATGGTG CTGCATGCCA AGTAAAGGAG GCTGAAATGT TCCACTGCCA GTGTCTTAGT 
GGTTATACAG GGCCAACCTG TGCAGATGAA CGGAACCCAT GTGACCCCAA CCCCTGCCAC 
GTTTCTGCTA CCTGTTTGGT TCTACCTGAG GGAGGTGCTA AATGTGAATG TCCAATGGGC 
AGAGAGGGAG AGTTTTGTGA GAAAGTGAGC AACCAGGATC TGAATTTGCC ATTCTTACCT 
CAGTTTAATG GATACTCTTA CTTGGAAATG AAGGGACTAC AGACATTTGC CACTGACCTA 
CAGGACAAGC TTGCCATGGA AGTAGTGTTC CTAGCCAGCA ACCCCAATGG CCTCATCTTC 
TATAATGGGC AGAAAACTGA TGGAAAAGGA GATTTTGTGT CACTGTCCCT TCATGATGGC 
AATCTAGAAT ACCGTTACGA TCTCGGAAAG GGAGCTGCTG TCATCAAGAG TAAGGAAAAG 
ATTCCACTCA ACACATGGCT CAGCGTCTCC CTTGAGCGTA GCGGCAGGAA GGGATTGATG 
CGGATCAATA ACATGGAACA AGTTACTGGG GAATCACCGA AATCCCGTAA GGCACCACAT 
ACCGCTCTCA ATCTGAAAGA GCCTCTATTT GTGGGTGGAG CTCCAGATTT CAGCAAATTA 
GCCAGGGCAG CGGCTGCCTC TACAAGCTTT GAAGGAGCCA TTCAGAAGAT TTCCATTAAG 
GGGCTGCCTG TTTTGAGAGA AGAAAACATC CGCAGTGCCA TTGAGATCTC TACTTTCCGC 
AGCCATCCTT GTACCAAGAA ACCCTTCCCT TGCCAGAATG GGGGGCTTTG TTCTCCTAAT 
AAAGGCAACT ATGAGTGTGT TTGCCAGAGG GGTTTCTCAG GCAGCCAGTG TGAAAAAGTG 
ACCATTGAGA AAGCAGCTGG AGAGTCTGAG GCAGTATCAT TTGATGGCAA GACTTACTTG 
GAATATCACA TTGCTGTAAC CAAAAGAACT GGTCCTGTGG ACAATCCAGA TGACCCCAGT 
GAAAAGGCGC TGCTAGTCAA CCAATTTGAG CTGAGCATCA AGACTGAAGC CACTCAGGGT 
CTCATCCTGT GGAGCGGGAA AGGGACAGAA CGTGCCTATT ATATTGCCTT GGCTGTAGTT 
GGAGGTTATG TTGAGATGAC CTATGACCTG GGCTCCAAGC CTGTTGTCCT ACGATCCACA 
GTACCTGTGA ACACCAACGA GTGGATTCAC ATCAAAGCTG TACGAGACAA CCGAGATGGC 
ATTTTACGAG TTGGCAATGA GGACCCCGTC CATGGATCCT CTCCTTTTGG AGTCAAAGAG 
CTGGACACTG ATGGAGCCCT ATGGCTGGGT GGAATAGAAA AGCTGAGTGC CTCATACAAG 
CTGCCCAAAG CGTACAGCAC GGGATTCATT GGCTGCATGA AGGACGTTCT GGTGGACCAA 
CAAGACCTGC ACTTAGTGGA AGATGCATTG AATAAACCAC CCATATTGTA CTGCTCAGCC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agrn ( XM_018096015.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951504.1 Xenopus tropicalis agrin (agrn), transcript variant X5, mRNA.

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CloneID	OXa45878
Clone ID Related Accession (Same CDS sequence)	XM_018096015.1 , XM_018096015.2
Accession Version	XM_018096015.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	agrin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGGTGAGATC CCTGACTGGG TGCCTCTAAT GGTTACAACC 
ACAACTACAA CGGCAGCAAC CACTAGCATG GTAGCTACTA CTGCTGTTGC CCCCACCACC 
ACAACCCCTG TAGTCGCTAC CACAACAACT GTGGCAAGAA CTCCACTACC TAGAAGAATA 
GTTGCAGTCA AGACCACCCC TGTGACAATC ACTCAGCAAC CTGTCACCAC TCCAAGAATG 
ACAACAAGAC GACCAGTAAC TACAAAAAGA CCAATGACCA CTATTCCTGC CACCACCAGG 
AGAAAGCCAC CACGTATTCA AGCAGTCACA AAAAAGCCAC CCCGGCCATG TGAATCACAC 
CCTTGTCTAC ATGGAGGTAC TTGTGAGGAT AATGGGAAGG ATTTCACCTG TAGCTGCCCA 
GCTGGCAGAG GAGGAGCTGT GTGCGAGAAG AGTATTAAGT ATTTTATTCC TGGGTTTGGT 
GGTAAGTCAT ACCTGGCATT CAAGATGATG AAGGCATATC ATACAGTGCG CATAGCCATG 
GAGTTCCGAT CCTCTGAGCA GAACGGGCTT CTCCTATACA ATGGTCAGTC ACTTGGGAAG 
GACTTTATCT CACTTGCAGT TAATAATGGC TTTGTGGAAC TCAGGTTTAA TACTGGATCT 
GGAACTGGAA TAATAACTAG TAAAGTGCCC ATTGAACCTG GCAAGTGGCA TCAGTTAGTA 
GTGAACCGTA ACAGACGCAG TGGCATGTTG TCTGTGGATG GTGAATCCCA TGTCAATGGT 
GAAAGTCCAT CTGGAACCGA TGGCCTCAAC CTAGACACAG ACCTGTTTAT AGGAGGAGCT 
CCTGAGGACC AGATGGCTGT GGTATCAGAA AGGACCTCTT TGTCTGTGGG GCTTAAAGGC 
TGCATCCGAC AACTTGATGT GAACAACCAA CTATACAACC TTCAAGAGGG AGGGGCTGAT 
GTCTTGTATG GAAGTGGGGT GGGAGAGTGT GGAAACAACC CTTGCCAGCC CAACCCATGC 
CATAATGGTG CTGCATGCCA AGTAAAGGAG GCTGAAATGT TCCACTGCCA GTGTCTTAGT 
GGTTATACAG GGCCAACCTG TGCAGATGAA CGGAACCCAT GTGACCCCAA CCCCTGCCAC 
GTTTCTGCTA CCTGTTTGGT TCTACCTGAG GGAGGTGCTA AATGTGAATG TCCAATGGGC 
AGAGAGGGAG AGTTTTGTGA GAAAGTGAGC AACCAGGATC TGAATTTGCC ATTCTTACCT 
CAGTTTAATG GATACTCTTA CTTGGAAATG AAGGGACTAC AGACATTTGC CACTGACCTA 
CAGGACAAGC TTGCCATGGA AGTAGTGTTC CTAGCCAGCA ACCCCAATGG CCTCATCTTC 
TATAATGGGC AGAAAACTGA TGGAAAAGGA GATTTTGTGT CACTGTCCCT TCATGATGGC 
AATCTAGAAT ACCGTTACGA TCTCGGAAAG GGAGCTGCTG TCATCAAGAG TAAGGAAAAG 
ATTCCACTCA ACACATGGCT CAGCGTCTCC CTTGAGCGTA GCGGCAGGAA GGGATTGATG 
CGGATCAATA ACATGGAACA AGTTACTGGG GAATCACCGA AATCCCGTAA GGCACCACAT 
ACCGCTCTCA ATCTGAAAGA GCCTCTATTT GTGGGTGGAG CTCCAGATTT CAGCAAATTA 
GCCAGGGCAG CGGCTGCCTC TACAAGCTTT GAAGGAGCCA TTCAGAAGAT TTCCATTAAG 
GGGCTGCCTG TTTTGAGAGA AGAAAACATC CGCAGTGCCA TTGAGATCTC TACTTTCCGC 
AGCCATCCTT GTACCAAGAA ACCCTTCCCT TGCCAGAATG GGGGGCTTTG TTCTCCTAAT 
AAAGGCAACT ATGAGTGTGT TTGCCAGAGG GGTTTCTCAG GCAGCCAGTG TGAAAAAGTG 
ACCATTGAGA AAGCAGCTGG AGAGTCTGAG GCAGTATCAT TTGATGGCAA GACTTACTTG 
GAATATCACA TTGCTGTAAC CAAAAGAACT GGTCCTGTGG ACAATCCAGA TGACCCCAGT 
GAAAAGGCGC TGCTAGTCAA CCAATTTGAG CTGAGCATCA AGACTGAAGC CACTCAGGGT 
CTCATCCTGT GGAGCGGGAA AGGGACAGAA CGTGCCTATT ATATTGCCTT GGCTGTAGTT 
GGAGGTTATG TTGAGATGAC CTATGACCTG GGCTCCAAGC CTGTTGTCCT ACGATCCACA 
GTACCTGTGA ACACCAACGA GTGGATTCAC ATCAAAGCTG TACGAGACAA CCGAGATGGC 
ATTTTACGAG TTGGCAATGA GGACCCCGTC CATGGATCCT CTCCTTTTGG AGTCAAAGAG 
CTGGACACTG ATGGAGCCCT ATGGCTGGGT GGAATAGAAA AGCTGAGTGC CTCATACAAG 
CTGCCCAAAG CGTACAGCAC GGGATTCATT GGCTGCATGA AGGACGTTCT GGTGGACCAA 
CAAGACCTGC ACTTAGTGGA AGATGCATTG AATAAACCAC CCATATTGTA CTGCTCAGCC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951504.1
CDS	304..6609
Translation	MGRRWGAPAVLLWTLWALAAVGLGFSRGPCPERELELREEEANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNKVMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEEHRRLSADKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACASIVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDPCSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRVNPRTRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQDKCKEDCKHNSVCLNRRGVTRCSCDRVTCDSTYKPVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCSFGAACVVKNKKAVCECQQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGAICEAETGRCVCPTECVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKCVNNQCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETVTHANLQTLSPTGTPSPPIVRTLPTPGTTKITKTKKKTDSFQLVLEATSLSPFSSTTMRPVTQTHRAHTSTVRPAITIPTVHPTVLQIYDESGSGSGDEELEASGDQDLSGVEPKEPFDVENEQKEEAEEKRDFVATTVIERASCYNTPLGCCSDGKTPANDAEGTNCPKTKIFQGVLIIEELEGQEVFYTPEMSDYKSELFGETARSIENALDNLLRNSDIKRDFKSIRVRDLGPSNSIRAIVEAHFDPATTMSAMDVQRALLRQLKSSKKRAITIKKPIQENVKFLDFGEIPDWVPLMVTTTTTTAATTSMVATTAVAPTTTTPVVATTTTVARTPLPRRIVAVKTTPVTITQQPVTTPRMTTRRPVTTKRPMTTIPATTRRKPPRIQAVTKKPPRPCESHPCLHGGTCEDNGKDFTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNLKEPLFVGGAPDFSKLARAAAASTSFEGAIQKISIKGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLCSPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKRTGPVDNPDDPSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKELDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDALNKPPILYCSAK

Target ORF information:

RefSeq Version	XM_018096015.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis agrin (agrn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096015.2

ORF Insert Sequence:

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGGTGAGATC CCTGACTGGG TGCCTCTAAT GGTTACAACC 
ACAACTACAA CGGCAGCAAC CACTAGCATG GTAGCTACTA CTGCTGTTGC CCCCACCACC 
ACAACCCCTG TAGTCGCTAC CACAACAACT GTGGCAAGAA CTCCACTACC TAGAAGAATA 
GTTGCAGTCA AGACCACCCC TGTGACAATC ACTCAGCAAC CTGTCACCAC TCCAAGAATG 
ACAACAAGAC GACCAGTAAC TACAAAAAGA CCAATGACCA CTATTCCTGC CACCACCAGG 
AGAAAGCCAC CACGTATTCA AGCAGTCACA AAAAAGCCAC CCCGGCCATG TGAATCACAC 
CCTTGTCTAC ATGGAGGTAC TTGTGAGGAT AATGGGAAGG ATTTCACCTG TAGCTGCCCA 
GCTGGCAGAG GAGGAGCTGT GTGCGAGAAG AGTATTAAGT ATTTTATTCC TGGGTTTGGT 
GGTAAGTCAT ACCTGGCATT CAAGATGATG AAGGCATATC ATACAGTGCG CATAGCCATG 
GAGTTCCGAT CCTCTGAGCA GAACGGGCTT CTCCTATACA ATGGTCAGTC ACTTGGGAAG 
GACTTTATCT CACTTGCAGT TAATAATGGC TTTGTGGAAC TCAGGTTTAA TACTGGATCT 
GGAACTGGAA TAATAACTAG TAAAGTGCCC ATTGAACCTG GCAAGTGGCA TCAGTTAGTA 
GTGAACCGTA ACAGACGCAG TGGCATGTTG TCTGTGGATG GTGAATCCCA TGTCAATGGT 
GAAAGTCCAT CTGGAACCGA TGGCCTCAAC CTAGACACAG ACCTGTTTAT AGGAGGAGCT 
CCTGAGGACC AGATGGCTGT GGTATCAGAA AGGACCTCTT TGTCTGTGGG GCTTAAAGGC 
TGCATCCGAC AACTTGATGT GAACAACCAA CTATACAACC TTCAAGAGGG AGGGGCTGAT 
GTCTTGTATG GAAGTGGGGT GGGAGAGTGT GGAAACAACC CTTGCCAGCC CAACCCATGC 
CATAATGGTG CTGCATGCCA AGTAAAGGAG GCTGAAATGT TCCACTGCCA GTGTCTTAGT 
GGTTATACAG GGCCAACCTG TGCAGATGAA CGGAACCCAT GTGACCCCAA CCCCTGCCAC 
GTTTCTGCTA CCTGTTTGGT TCTACCTGAG GGAGGTGCTA AATGTGAATG TCCAATGGGC 
AGAGAGGGAG AGTTTTGTGA GAAAGTGAGC AACCAGGATC TGAATTTGCC ATTCTTACCT 
CAGTTTAATG GATACTCTTA CTTGGAAATG AAGGGACTAC AGACATTTGC CACTGACCTA 
CAGGACAAGC TTGCCATGGA AGTAGTGTTC CTAGCCAGCA ACCCCAATGG CCTCATCTTC 
TATAATGGGC AGAAAACTGA TGGAAAAGGA GATTTTGTGT CACTGTCCCT TCATGATGGC 
AATCTAGAAT ACCGTTACGA TCTCGGAAAG GGAGCTGCTG TCATCAAGAG TAAGGAAAAG 
ATTCCACTCA ACACATGGCT CAGCGTCTCC CTTGAGCGTA GCGGCAGGAA GGGATTGATG 
CGGATCAATA ACATGGAACA AGTTACTGGG GAATCACCGA AATCCCGTAA GGCACCACAT 
ACCGCTCTCA ATCTGAAAGA GCCTCTATTT GTGGGTGGAG CTCCAGATTT CAGCAAATTA 
GCCAGGGCAG CGGCTGCCTC TACAAGCTTT GAAGGAGCCA TTCAGAAGAT TTCCATTAAG 
GGGCTGCCTG TTTTGAGAGA AGAAAACATC CGCAGTGCCA TTGAGATCTC TACTTTCCGC 
AGCCATCCTT GTACCAAGAA ACCCTTCCCT TGCCAGAATG GGGGGCTTTG TTCTCCTAAT 
AAAGGCAACT ATGAGTGTGT TTGCCAGAGG GGTTTCTCAG GCAGCCAGTG TGAAAAAGTG 
ACCATTGAGA AAGCAGCTGG AGAGTCTGAG GCAGTATCAT TTGATGGCAA GACTTACTTG 
GAATATCACA TTGCTGTAAC CAAAAGAACT GGTCCTGTGG ACAATCCAGA TGACCCCAGT 
GAAAAGGCGC TGCTAGTCAA CCAATTTGAG CTGAGCATCA AGACTGAAGC CACTCAGGGT 
CTCATCCTGT GGAGCGGGAA AGGGACAGAA CGTGCCTATT ATATTGCCTT GGCTGTAGTT 
GGAGGTTATG TTGAGATGAC CTATGACCTG GGCTCCAAGC CTGTTGTCCT ACGATCCACA 
GTACCTGTGA ACACCAACGA GTGGATTCAC ATCAAAGCTG TACGAGACAA CCGAGATGGC 
ATTTTACGAG TTGGCAATGA GGACCCCGTC CATGGATCCT CTCCTTTTGG AGTCAAAGAG 
CTGGACACTG ATGGAGCCCT ATGGCTGGGT GGAATAGAAA AGCTGAGTGC CTCATACAAG 
CTGCCCAAAG CGTACAGCAC GGGATTCATT GGCTGCATGA AGGACGTTCT GGTGGACCAA 
CAAGACCTGC ACTTAGTGGA AGATGCATTG AATAAACCAC CCATATTGTA CTGCTCAGCC 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agrn ( XM_018096016.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951505.1 Xenopus tropicalis agrin (agrn), transcript variant X6, mRNA.

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CloneID	OXa45879
Clone ID Related Accession (Same CDS sequence)	XM_018096016.2 , XM_018096016.1
Accession Version	XM_018096016.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	agrin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096016.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGGTGAGATC CCTGACTGGG TGCCTCTAAT GGTTACAACC 
ACAACTACAA CGGCAGCAAC CACTAGCATG GTAGCTACTA CTGCTGTTGC CCCCACCACC 
ACAACCCCTG TAGTCGCTAC CACAACAACT GTGGCAAGAA CTCCACTACC TAGAAGAATA 
GTTGCAGTCA AGACCACCCC TGTGACAATC ACTCAGCAAC CTGTCACCAC TCCAAGAATG 
ACAACAAGAC GACCAGTAAC TACAAAAAGA CCAATGACCA CTATTCCTGC CACCACCAGG 
AGAAAGCCAC CACGTATTCA AGCAGTCACA AAAAAGCCAC CCCGGCCATG TGAATCACAC 
CCTTGTCTAC ATGGAGGTAC TTGTGAGGAT AATGGGAAGG ATTTCACCTG TAGCTGCCCA 
GCTGGCAGAG GAGGAGCTGT GTGCGAGAAG AGTATTAAGT ATTTTATTCC TGGGTTTGGT 
GGTAAGTCAT ACCTGGCATT CAAGATGATG AAGGCATATC ATACAGTGCG CATAGCCATG 
GAGTTCCGAT CCTCTGAGCA GAACGGGCTT CTCCTATACA ATGGTCAGTC ACTTGGGAAG 
GACTTTATCT CACTTGCAGT TAATAATGGC TTTGTGGAAC TCAGGTTTAA TACTGGATCT 
GGAACTGGAA TAATAACTAG TAAAGTGCCC ATTGAACCTG GCAAGTGGCA TCAGTTAGTA 
GTGAACCGTA ACAGACGCAG TGGCATGTTG TCTGTGGATG GTGAATCCCA TGTCAATGGT 
GAAAGTCCAT CTGGAACCGA TGGCCTCAAC CTAGACACAG ACCTGTTTAT AGGAGGAGCT 
CCTGAGGACC AGATGGCTGT GGTATCAGAA AGGACCTCTT TGTCTGTGGG GCTTAAAGGC 
TGCATCCGAC AACTTGATGT GAACAACCAA CTATACAACC TTCAAGAGGG AGGGGCTGAT 
GTCTTGTATG GAAGTGGGGT GGGAGAGTGT GGAAACAACC CTTGCCAGCC CAACCCATGC 
CATAATGGTG CTGCATGCCA AGTAAAGGAG GCTGAAATGT TCCACTGCCA GTGTCTTAGT 
GGTTATACAG GGCCAACCTG TGCAGATGAA CGGAACCCAT GTGACCCCAA CCCCTGCCAC 
GTTTCTGCTA CCTGTTTGGT TCTACCTGAG GGAGGTGCTA AATGTGAATG TCCAATGGGC 
AGAGAGGGAG AGTTTTGTGA GAAAGTGAGC AACCAGGATC TGAATTTGCC ATTCTTACCT 
CAGTTTAATG GATACTCTTA CTTGGAAATG AAGGGACTAC AGACATTTGC CACTGACCTA 
CAGGACAAGC TTGCCATGGA AGTAGTGTTC CTAGCCAGCA ACCCCAATGG CCTCATCTTC 
TATAATGGGC AGAAAACTGA TGGAAAAGGA GATTTTGTGT CACTGTCCCT TCATGATGGC 
AATCTAGAAT ACCGTTACGA TCTCGGAAAG GGAGCTGCTG TCATCAAGAG TAAGGAAAAG 
ATTCCACTCA ACACATGGCT CAGCGTCTCC CTTGAGCGTA GCGGCAGGAA GGGATTGATG 
CGGATCAATA ACATGGAACA AGTTACTGGG GAATCACCGA AATCCCGTAA GGCACCACAT 
ACCGCTCTCA ATCTGAAAGA GCCTCTATTT GTGGGTGGAG CTCCAGATTT CAGCAAATTA 
GCCAGGGCAG CGGCTGCCTC TACAAGCTTT GAAGGAGCCA TTCAGAAGAT TTCCATTAAG 
GGGCTGCCTG TTTTGAGAGA AGAAAACATC CGCAGTGCCA TTGAGATCTC TACTTTCCGC 
AGCCATCCTT GTACCAAGAA ACCCTTCCCT TGCCAGAATG GGGGGCTTTG TTCTCCTAAT 
AAAGGCAACT ATGAGTGTGT TTGCCAGAGG GGTTTCTCAG GCAGCCAGTG TGAAAAAGTG 
ACCATTGAGA AAGCAGCTGG AGAGTCTGAG GCAGTATCAT TTGATGGCAA GACTTACTTG 
GAATATCACA TTGCTGTAAC CAAAAGTGAA AAGGCGCTGC TAGTCAACCA ATTTGAGCTG 
AGCATCAAGA CTGAAGCCAC TCAGGGTCTC ATCCTGTGGA GCGGGAAAGG GACAGAACGT 
GCCTATTATA TTGCCTTGGC TGTAGTTGGA GGTTATGTTG AGATGACCTA TGACCTGGGC 
TCCAAGCCTG TTGTCCTACG ATCCACAGTA CCTGTGAACA CCAACGAGTG GATTCACATC 
AAAGCTGTAC GAGACAACCG AGATGGCATT TTACGAGTTG GCAATGAGGA CCCCGTCCAT 
GGATCCTCTC CTTTTGGAGT CAAAGAGCTG GACACTGATG GAGCCCTATG GCTGGGTGGA 
ATAGAAAAGC TGAGTGCCTC ATACAAGCTG CCCAAAGCGT ACAGCACGGG ATTCATTGGC 
TGCATGAAGG ACGTTCTGGT GGACCAACAA GACCTGCACT TAGTGGAAGA TGCATTGAAT 
AAACCACCCA TATTGTACTG CTCAGCCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951505.1
CDS	303..6575
Translation	MGRRWGAPAVLLWTLWALAAVGLGFSRGPCPERELELREEEANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNKVMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEEHRRLSADKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACASIVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDPCSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRVNPRTRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQDKCKEDCKHNSVCLNRRGVTRCSCDRVTCDSTYKPVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCSFGAACVVKNKKAVCECQQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGAICEAETGRCVCPTECVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKCVNNQCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETVTHANLQTLSPTGTPSPPIVRTLPTPGTTKITKTKKKTDSFQLVLEATSLSPFSSTTMRPVTQTHRAHTSTVRPAITIPTVHPTVLQIYDESGSGSGDEELEASGDQDLSGVEPKEPFDVENEQKEEAEEKRDFVATTVIERASCYNTPLGCCSDGKTPANDAEGTNCPKTKIFQGVLIIEELEGQEVFYTPEMSDYKSELFGETARSIENALDNLLRNSDIKRDFKSIRVRDLGPSNSIRAIVEAHFDPATTMSAMDVQRALLRQLKSSKKRAITIKKPIQENVKFLDFGEIPDWVPLMVTTTTTTAATTSMVATTAVAPTTTTPVVATTTTVARTPLPRRIVAVKTTPVTITQQPVTTPRMTTRRPVTTKRPMTTIPATTRRKPPRIQAVTKKPPRPCESHPCLHGGTCEDNGKDFTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNLKEPLFVGGAPDFSKLARAAAASTSFEGAIQKISIKGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLCSPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKELDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDALNKPPILYCSAK

Target ORF information:

RefSeq Version	XM_018096016.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis agrin (agrn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096016.2

ORF Insert Sequence:

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGGTGAGATC CCTGACTGGG TGCCTCTAAT GGTTACAACC 
ACAACTACAA CGGCAGCAAC CACTAGCATG GTAGCTACTA CTGCTGTTGC CCCCACCACC 
ACAACCCCTG TAGTCGCTAC CACAACAACT GTGGCAAGAA CTCCACTACC TAGAAGAATA 
GTTGCAGTCA AGACCACCCC TGTGACAATC ACTCAGCAAC CTGTCACCAC TCCAAGAATG 
ACAACAAGAC GACCAGTAAC TACAAAAAGA CCAATGACCA CTATTCCTGC CACCACCAGG 
AGAAAGCCAC CACGTATTCA AGCAGTCACA AAAAAGCCAC CCCGGCCATG TGAATCACAC 
CCTTGTCTAC ATGGAGGTAC TTGTGAGGAT AATGGGAAGG ATTTCACCTG TAGCTGCCCA 
GCTGGCAGAG GAGGAGCTGT GTGCGAGAAG AGTATTAAGT ATTTTATTCC TGGGTTTGGT 
GGTAAGTCAT ACCTGGCATT CAAGATGATG AAGGCATATC ATACAGTGCG CATAGCCATG 
GAGTTCCGAT CCTCTGAGCA GAACGGGCTT CTCCTATACA ATGGTCAGTC ACTTGGGAAG 
GACTTTATCT CACTTGCAGT TAATAATGGC TTTGTGGAAC TCAGGTTTAA TACTGGATCT 
GGAACTGGAA TAATAACTAG TAAAGTGCCC ATTGAACCTG GCAAGTGGCA TCAGTTAGTA 
GTGAACCGTA ACAGACGCAG TGGCATGTTG TCTGTGGATG GTGAATCCCA TGTCAATGGT 
GAAAGTCCAT CTGGAACCGA TGGCCTCAAC CTAGACACAG ACCTGTTTAT AGGAGGAGCT 
CCTGAGGACC AGATGGCTGT GGTATCAGAA AGGACCTCTT TGTCTGTGGG GCTTAAAGGC 
TGCATCCGAC AACTTGATGT GAACAACCAA CTATACAACC TTCAAGAGGG AGGGGCTGAT 
GTCTTGTATG GAAGTGGGGT GGGAGAGTGT GGAAACAACC CTTGCCAGCC CAACCCATGC 
CATAATGGTG CTGCATGCCA AGTAAAGGAG GCTGAAATGT TCCACTGCCA GTGTCTTAGT 
GGTTATACAG GGCCAACCTG TGCAGATGAA CGGAACCCAT GTGACCCCAA CCCCTGCCAC 
GTTTCTGCTA CCTGTTTGGT TCTACCTGAG GGAGGTGCTA AATGTGAATG TCCAATGGGC 
AGAGAGGGAG AGTTTTGTGA GAAAGTGAGC AACCAGGATC TGAATTTGCC ATTCTTACCT 
CAGTTTAATG GATACTCTTA CTTGGAAATG AAGGGACTAC AGACATTTGC CACTGACCTA 
CAGGACAAGC TTGCCATGGA AGTAGTGTTC CTAGCCAGCA ACCCCAATGG CCTCATCTTC 
TATAATGGGC AGAAAACTGA TGGAAAAGGA GATTTTGTGT CACTGTCCCT TCATGATGGC 
AATCTAGAAT ACCGTTACGA TCTCGGAAAG GGAGCTGCTG TCATCAAGAG TAAGGAAAAG 
ATTCCACTCA ACACATGGCT CAGCGTCTCC CTTGAGCGTA GCGGCAGGAA GGGATTGATG 
CGGATCAATA ACATGGAACA AGTTACTGGG GAATCACCGA AATCCCGTAA GGCACCACAT 
ACCGCTCTCA ATCTGAAAGA GCCTCTATTT GTGGGTGGAG CTCCAGATTT CAGCAAATTA 
GCCAGGGCAG CGGCTGCCTC TACAAGCTTT GAAGGAGCCA TTCAGAAGAT TTCCATTAAG 
GGGCTGCCTG TTTTGAGAGA AGAAAACATC CGCAGTGCCA TTGAGATCTC TACTTTCCGC 
AGCCATCCTT GTACCAAGAA ACCCTTCCCT TGCCAGAATG GGGGGCTTTG TTCTCCTAAT 
AAAGGCAACT ATGAGTGTGT TTGCCAGAGG GGTTTCTCAG GCAGCCAGTG TGAAAAAGTG 
ACCATTGAGA AAGCAGCTGG AGAGTCTGAG GCAGTATCAT TTGATGGCAA GACTTACTTG 
GAATATCACA TTGCTGTAAC CAAAAGTGAA AAGGCGCTGC TAGTCAACCA ATTTGAGCTG 
AGCATCAAGA CTGAAGCCAC TCAGGGTCTC ATCCTGTGGA GCGGGAAAGG GACAGAACGT 
GCCTATTATA TTGCCTTGGC TGTAGTTGGA GGTTATGTTG AGATGACCTA TGACCTGGGC 
TCCAAGCCTG TTGTCCTACG ATCCACAGTA CCTGTGAACA CCAACGAGTG GATTCACATC 
AAAGCTGTAC GAGACAACCG AGATGGCATT TTACGAGTTG GCAATGAGGA CCCCGTCCAT 
GGATCCTCTC CTTTTGGAGT CAAAGAGCTG GACACTGATG GAGCCCTATG GCTGGGTGGA 
ATAGAAAAGC TGAGTGCCTC ATACAAGCTG CCCAAAGCGT ACAGCACGGG ATTCATTGGC 
TGCATGAAGG ACGTTCTGGT GGACCAACAA GACCTGCACT TAGTGGAAGA TGCATTGAAT 
AAACCACCCA TATTGTACTG CTCAGCCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agrn ( XM_018096016.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951505.1 Xenopus tropicalis agrin (agrn), transcript variant X6, mRNA.

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CloneID	OXa45879
Clone ID Related Accession (Same CDS sequence)	XM_018096016.2 , XM_018096016.1
Accession Version	XM_018096016.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	agrin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGGTGAGATC CCTGACTGGG TGCCTCTAAT GGTTACAACC 
ACAACTACAA CGGCAGCAAC CACTAGCATG GTAGCTACTA CTGCTGTTGC CCCCACCACC 
ACAACCCCTG TAGTCGCTAC CACAACAACT GTGGCAAGAA CTCCACTACC TAGAAGAATA 
GTTGCAGTCA AGACCACCCC TGTGACAATC ACTCAGCAAC CTGTCACCAC TCCAAGAATG 
ACAACAAGAC GACCAGTAAC TACAAAAAGA CCAATGACCA CTATTCCTGC CACCACCAGG 
AGAAAGCCAC CACGTATTCA AGCAGTCACA AAAAAGCCAC CCCGGCCATG TGAATCACAC 
CCTTGTCTAC ATGGAGGTAC TTGTGAGGAT AATGGGAAGG ATTTCACCTG TAGCTGCCCA 
GCTGGCAGAG GAGGAGCTGT GTGCGAGAAG AGTATTAAGT ATTTTATTCC TGGGTTTGGT 
GGTAAGTCAT ACCTGGCATT CAAGATGATG AAGGCATATC ATACAGTGCG CATAGCCATG 
GAGTTCCGAT CCTCTGAGCA GAACGGGCTT CTCCTATACA ATGGTCAGTC ACTTGGGAAG 
GACTTTATCT CACTTGCAGT TAATAATGGC TTTGTGGAAC TCAGGTTTAA TACTGGATCT 
GGAACTGGAA TAATAACTAG TAAAGTGCCC ATTGAACCTG GCAAGTGGCA TCAGTTAGTA 
GTGAACCGTA ACAGACGCAG TGGCATGTTG TCTGTGGATG GTGAATCCCA TGTCAATGGT 
GAAAGTCCAT CTGGAACCGA TGGCCTCAAC CTAGACACAG ACCTGTTTAT AGGAGGAGCT 
CCTGAGGACC AGATGGCTGT GGTATCAGAA AGGACCTCTT TGTCTGTGGG GCTTAAAGGC 
TGCATCCGAC AACTTGATGT GAACAACCAA CTATACAACC TTCAAGAGGG AGGGGCTGAT 
GTCTTGTATG GAAGTGGGGT GGGAGAGTGT GGAAACAACC CTTGCCAGCC CAACCCATGC 
CATAATGGTG CTGCATGCCA AGTAAAGGAG GCTGAAATGT TCCACTGCCA GTGTCTTAGT 
GGTTATACAG GGCCAACCTG TGCAGATGAA CGGAACCCAT GTGACCCCAA CCCCTGCCAC 
GTTTCTGCTA CCTGTTTGGT TCTACCTGAG GGAGGTGCTA AATGTGAATG TCCAATGGGC 
AGAGAGGGAG AGTTTTGTGA GAAAGTGAGC AACCAGGATC TGAATTTGCC ATTCTTACCT 
CAGTTTAATG GATACTCTTA CTTGGAAATG AAGGGACTAC AGACATTTGC CACTGACCTA 
CAGGACAAGC TTGCCATGGA AGTAGTGTTC CTAGCCAGCA ACCCCAATGG CCTCATCTTC 
TATAATGGGC AGAAAACTGA TGGAAAAGGA GATTTTGTGT CACTGTCCCT TCATGATGGC 
AATCTAGAAT ACCGTTACGA TCTCGGAAAG GGAGCTGCTG TCATCAAGAG TAAGGAAAAG 
ATTCCACTCA ACACATGGCT CAGCGTCTCC CTTGAGCGTA GCGGCAGGAA GGGATTGATG 
CGGATCAATA ACATGGAACA AGTTACTGGG GAATCACCGA AATCCCGTAA GGCACCACAT 
ACCGCTCTCA ATCTGAAAGA GCCTCTATTT GTGGGTGGAG CTCCAGATTT CAGCAAATTA 
GCCAGGGCAG CGGCTGCCTC TACAAGCTTT GAAGGAGCCA TTCAGAAGAT TTCCATTAAG 
GGGCTGCCTG TTTTGAGAGA AGAAAACATC CGCAGTGCCA TTGAGATCTC TACTTTCCGC 
AGCCATCCTT GTACCAAGAA ACCCTTCCCT TGCCAGAATG GGGGGCTTTG TTCTCCTAAT 
AAAGGCAACT ATGAGTGTGT TTGCCAGAGG GGTTTCTCAG GCAGCCAGTG TGAAAAAGTG 
ACCATTGAGA AAGCAGCTGG AGAGTCTGAG GCAGTATCAT TTGATGGCAA GACTTACTTG 
GAATATCACA TTGCTGTAAC CAAAAGTGAA AAGGCGCTGC TAGTCAACCA ATTTGAGCTG 
AGCATCAAGA CTGAAGCCAC TCAGGGTCTC ATCCTGTGGA GCGGGAAAGG GACAGAACGT 
GCCTATTATA TTGCCTTGGC TGTAGTTGGA GGTTATGTTG AGATGACCTA TGACCTGGGC 
TCCAAGCCTG TTGTCCTACG ATCCACAGTA CCTGTGAACA CCAACGAGTG GATTCACATC 
AAAGCTGTAC GAGACAACCG AGATGGCATT TTACGAGTTG GCAATGAGGA CCCCGTCCAT 
GGATCCTCTC CTTTTGGAGT CAAAGAGCTG GACACTGATG GAGCCCTATG GCTGGGTGGA 
ATAGAAAAGC TGAGTGCCTC ATACAAGCTG CCCAAAGCGT ACAGCACGGG ATTCATTGGC 
TGCATGAAGG ACGTTCTGGT GGACCAACAA GACCTGCACT TAGTGGAAGA TGCATTGAAT 
AAACCACCCA TATTGTACTG CTCAGCCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951505.1
CDS	676..6948
Translation	MGRRWGAPAVLLWTLWALAAVGLGFSRGPCPERELELREEEANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNKVMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEEHRRLSADKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACASIVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDPCSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRVNPRTRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQDKCKEDCKHNSVCLNRRGVTRCSCDRVTCDSTYKPVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCSFGAACVVKNKKAVCECQQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGAICEAETGRCVCPTECVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKCVNNQCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETVTHANLQTLSPTGTPSPPIVRTLPTPGTTKITKTKKKTDSFQLVLEATSLSPFSSTTMRPVTQTHRAHTSTVRPAITIPTVHPTVLQIYDESGSGSGDEELEASGDQDLSGVEPKEPFDVENEQKEEAEEKRDFVATTVIERASCYNTPLGCCSDGKTPANDAEGTNCPKTKIFQGVLIIEELEGQEVFYTPEMSDYKSELFGETARSIENALDNLLRNSDIKRDFKSIRVRDLGPSNSIRAIVEAHFDPATTMSAMDVQRALLRQLKSSKKRAITIKKPIQENVKFLDFGEIPDWVPLMVTTTTTTAATTSMVATTAVAPTTTTPVVATTTTVARTPLPRRIVAVKTTPVTITQQPVTTPRMTTRRPVTTKRPMTTIPATTRRKPPRIQAVTKKPPRPCESHPCLHGGTCEDNGKDFTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNLKEPLFVGGAPDFSKLARAAAASTSFEGAIQKISIKGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLCSPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKELDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDALNKPPILYCSAK

Target ORF information:

RefSeq Version	XM_018096016.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis agrin (agrn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096016.1

ORF Insert Sequence:

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG AGCACAGGAG GCTTAGCGCT 
GATAAACCTG GCCCTGTCCC TAATGCTCAG CCAACTCTAC AAGATGTGTG CCGAGGAATG 
CTCTGCGGAT TTGGCGCAGT ATGTGAGAAA AGCAGCACCG ACCCATCTCA GGCAACCTGC 
ATCTGTAAGA AAACCGCCTG CGCCTCCATT GTTGCCCCTG TGTGCGGTTC CGACTATTCT 
ACTTACAGCA ATGAGTGTGA ACTGGAGAGA GCCCAGTGCA ACCAGCAGAG GAGAATCAAA 
GTCATCAGCA AGGGGCCATG CGGCACCAAG GACCCATGCT CAACAGTGTC GTGTAGCTAT 
GGCAGTACCT GTGTCCGCTC AACAGATGGC CAGACGGCAA AGTGTATCTG TCCTGCTACC 
TGCACAGGGG TTCCAGAAAA TGTTGTTTGT GGCAGTGACG GCAAAGATTA TCACAATGAG 
TGTCAGCTTA ACAAGCAGGC ATGCGACAAT CAGGAGAATC TCTACAAGAA GTTTGATGGG 
CGTTGTGATC CATGCAGAAT TGCCATGAAT AATCAAAACA AAGCATGTCG AGTAAACCCA 
CGAACTAGGC AACTGGAAAT GCAATACCGA CCAGAATACT GCCCAGTCCG CAGAGATCCT 
GTTTGCGCAG ATGACGGTGT GACTTATGAC AATGATTGTG TAATGACTCG ATCTGGATTC 
ATCCGTGGCA TTGAACTGCA GAAGGTGCAC TCAGGGCAGT GCCAACCGCA AGACAAATGC 
AAGGAGGACT GTAAACACAA TTCTGTGTGT CTTAACCGGA GAGGTGTGAC GCGTTGTTCT 
TGCGACCGTG TTACTTGTGA TAGCACTTAC AAACCAGTCT GTGGTGGGGA CAGTCGCACG 
TATGCCAATG ACTGCGAGAG ACAAAAGGCA GAATGCTTTC AGAAAGCTAC CATTCCAGTA 
AAACACAAAG GCCCATGTGA TCTCCATTCC CCCAGTCCAT GCCTGAACAC AACCTGCAGT 
TTTGGAGCAG CATGTGTAGT AAAAAACAAG AAGGCAGTAT GCGAATGCCA GCAGGTGTGC 
CAGGCAGTAT ATGACCCAGT GTGTGGCAGT GATAAGCGCA CTTACGGCAA CCCATGTGAA 
CTGCAGTCAA CAGCCTGCAC GCTTAAAAAA GAAATAACCA TTTTACACAA GGGGCCTTGT 
GACCGCTGTG GGAAATGCCA GTTTGGAGCC ATCTGTGAAG CAGAGACTGG CCGCTGTGTA 
TGTCCAACAG AATGTGTGCC TTCCGCTCAG CCTGTTTGTG GCACTGATGG AAATACATAC 
GAAAGCGAGT GTGAGCTCCA TGTACGAGCA TGCACTCAGC AGATTAATCT ACAAGTTGCA 
GCCCAAGGAG ACTGCAAAAC CTGTGGCAAT ACTCTTTGTA CTTTTGGTGC CAAATGTGTG 
AACAACCAGT GTGTGTGCCT AACATGCGAG CGGCAACCCT ACCTACCAGT GTGCGGCAGC 
AATGGCGTCA CTTATGATAA CCAATGTGAA CTGAAAGCTG CATCCTGTAA GCAGATGAAG 
CTGATTGAAG TATCTCGGGC AGGATCTTGT GAAGACGAAT GCGGCTCTGG CACTGACGGT 
TCTGGTTCTG GTGAGGACAG CGAGTGTGAG CAGGATCGAT GCAGGCGCTA TGGTGGCTCA 
TGGGATGAAG ATGCAGAAGA TGATCGCTGC GTCTGTGACT TTACCTGTCA TGCTGTGCTT 
CGAATTTTGG TCTGTGGATC GGATGGCATT ACCTACTCTA ATGAGTGTGA GCTAAAGAAA 
ACTCGGTGTG AGAAACGTCA GGATCTGTAT GTGGCTGGAC AGGGTGCCTG TCGAGCTGCC 
ACCACCAGCT CTCCTCCTTT GGTGCCACAC TGCAGTCACA CTGTGTATGG CTGTTGTCCT 
GACAATATCA CAGCTGCCCT TGGCGTTGGC CTTGCCGGAT GCCCAAGCAG CTGTCAGTGC 
CACCAGTATG GCTCCTATGG AGGAACCTGT GATCCCACCA CTGGTCAGTG TTCTTGTAAA 
CCAGGCGTGG GGGGACTGAA ATGCGATAGA TGTGAGCCCG GGTTTTGGAA CTTCCGGGGC 
ATTGTTACGG ATAAGAAAAG CGGCTGCACC CCATGTAACT GTGACCCAGT TGGTGCCGTT 
CGAGATGACT GTGAGCAAAT GACAGGACTT TGTTCTTGCA AAACGGGAAT CTCAGGTTTG 
AAGTGCAGCC AGTGCCCTGA TGGACGCAAG CCTGGCCTTA CAGGCTGTGA GAAAGATCCT 
TCAGCTCCTA AATCTTGTGA TGCCATCAAG TGCCAATTTG GTGCAACTTG TGTGGTAATT 
GGAGGATTTG CTCATTGCGA GTGCCCATCT CCCCTGTGTG CTGAGGCGAA TCTGACAAAG 
GTCTGTGGTT CCGATGATGT GACTTACGGA GACTGGTGCC AACTGAAAAC CATTGCTTGC 
CGTCAGGGCC GCACTATCAC AGTGAAGCAC ACTGGTCCGT GCCAAGAAAC TGTCACTCAT 
GCCAACCTTC AGACATTGAG TCCAACAGGA ACACCCTCTC CGCCCATAGT TCGGACTCTT 
CCAACTCCAG GAACCACAAA GATAACAAAG ACCAAGAAAA AGACAGATTC CTTCCAGTTA 
GTTTTAGAAG CCACATCGCT CAGCCCATTT TCCAGCACCA CAATGAGACC TGTGACCCAG 
ACGCATAGAG CTCACACTTC TACTGTCAGG CCAGCAATCA CTATTCCCAC AGTCCATCCC 
ACTGTGCTTC AGATTTATGA TGAATCAGGA AGTGGAAGTG GGGATGAGGA GCTAGAAGCT 
AGCGGTGATC AAGATTTAAG TGGTGTTGAA CCCAAAGAGC CATTTGATGT GGAGAATGAA 
CAGAAGGAAG AAGCTGAAGA GAAGAGAGAT TTTGTAGCCA CCACGGTCAT AGAAAGAGCC 
AGCTGCTATA ACACACCTCT AGGATGCTGC TCTGATGGAA AGACCCCAGC TAATGATGCT 
GAAGGAACAA ATTGCCCAAA AACCAAAATT TTCCAAGGTG TCCTCATTAT TGAAGAGTTG 
GAGGGGCAAG AGGTTTTCTA CACACCAGAA ATGTCAGATT ACAAGTCTGA GCTCTTTGGG 
GAAACTGCTA GAAGCATTGA GAATGCACTG GATAATCTAC TTCGAAATTC AGACATCAAG 
AGGGATTTCA AGAGCATCCG TGTGAGGGAC CTTGGACCAA GCAATTCCAT CAGAGCCATT 
GTGGAAGCAC ATTTTGATCC AGCTACCACC ATGTCTGCTA TGGATGTTCA GAGAGCATTG 
CTGCGACAGC TCAAGAGCTC CAAGAAGAGA GCAATAACCA TAAAGAAGCC TATTCAGGAA 
AATGTCAAGT TCCTGGACTT TGGTGAGATC CCTGACTGGG TGCCTCTAAT GGTTACAACC 
ACAACTACAA CGGCAGCAAC CACTAGCATG GTAGCTACTA CTGCTGTTGC CCCCACCACC 
ACAACCCCTG TAGTCGCTAC CACAACAACT GTGGCAAGAA CTCCACTACC TAGAAGAATA 
GTTGCAGTCA AGACCACCCC TGTGACAATC ACTCAGCAAC CTGTCACCAC TCCAAGAATG 
ACAACAAGAC GACCAGTAAC TACAAAAAGA CCAATGACCA CTATTCCTGC CACCACCAGG 
AGAAAGCCAC CACGTATTCA AGCAGTCACA AAAAAGCCAC CCCGGCCATG TGAATCACAC 
CCTTGTCTAC ATGGAGGTAC TTGTGAGGAT AATGGGAAGG ATTTCACCTG TAGCTGCCCA 
GCTGGCAGAG GAGGAGCTGT GTGCGAGAAG AGTATTAAGT ATTTTATTCC TGGGTTTGGT 
GGTAAGTCAT ACCTGGCATT CAAGATGATG AAGGCATATC ATACAGTGCG CATAGCCATG 
GAGTTCCGAT CCTCTGAGCA GAACGGGCTT CTCCTATACA ATGGTCAGTC ACTTGGGAAG 
GACTTTATCT CACTTGCAGT TAATAATGGC TTTGTGGAAC TCAGGTTTAA TACTGGATCT 
GGAACTGGAA TAATAACTAG TAAAGTGCCC ATTGAACCTG GCAAGTGGCA TCAGTTAGTA 
GTGAACCGTA ACAGACGCAG TGGCATGTTG TCTGTGGATG GTGAATCCCA TGTCAATGGT 
GAAAGTCCAT CTGGAACCGA TGGCCTCAAC CTAGACACAG ACCTGTTTAT AGGAGGAGCT 
CCTGAGGACC AGATGGCTGT GGTATCAGAA AGGACCTCTT TGTCTGTGGG GCTTAAAGGC 
TGCATCCGAC AACTTGATGT GAACAACCAA CTATACAACC TTCAAGAGGG AGGGGCTGAT 
GTCTTGTATG GAAGTGGGGT GGGAGAGTGT GGAAACAACC CTTGCCAGCC CAACCCATGC 
CATAATGGTG CTGCATGCCA AGTAAAGGAG GCTGAAATGT TCCACTGCCA GTGTCTTAGT 
GGTTATACAG GGCCAACCTG TGCAGATGAA CGGAACCCAT GTGACCCCAA CCCCTGCCAC 
GTTTCTGCTA CCTGTTTGGT TCTACCTGAG GGAGGTGCTA AATGTGAATG TCCAATGGGC 
AGAGAGGGAG AGTTTTGTGA GAAAGTGAGC AACCAGGATC TGAATTTGCC ATTCTTACCT 
CAGTTTAATG GATACTCTTA CTTGGAAATG AAGGGACTAC AGACATTTGC CACTGACCTA 
CAGGACAAGC TTGCCATGGA AGTAGTGTTC CTAGCCAGCA ACCCCAATGG CCTCATCTTC 
TATAATGGGC AGAAAACTGA TGGAAAAGGA GATTTTGTGT CACTGTCCCT TCATGATGGC 
AATCTAGAAT ACCGTTACGA TCTCGGAAAG GGAGCTGCTG TCATCAAGAG TAAGGAAAAG 
ATTCCACTCA ACACATGGCT CAGCGTCTCC CTTGAGCGTA GCGGCAGGAA GGGATTGATG 
CGGATCAATA ACATGGAACA AGTTACTGGG GAATCACCGA AATCCCGTAA GGCACCACAT 
ACCGCTCTCA ATCTGAAAGA GCCTCTATTT GTGGGTGGAG CTCCAGATTT CAGCAAATTA 
GCCAGGGCAG CGGCTGCCTC TACAAGCTTT GAAGGAGCCA TTCAGAAGAT TTCCATTAAG 
GGGCTGCCTG TTTTGAGAGA AGAAAACATC CGCAGTGCCA TTGAGATCTC TACTTTCCGC 
AGCCATCCTT GTACCAAGAA ACCCTTCCCT TGCCAGAATG GGGGGCTTTG TTCTCCTAAT 
AAAGGCAACT ATGAGTGTGT TTGCCAGAGG GGTTTCTCAG GCAGCCAGTG TGAAAAAGTG 
ACCATTGAGA AAGCAGCTGG AGAGTCTGAG GCAGTATCAT TTGATGGCAA GACTTACTTG 
GAATATCACA TTGCTGTAAC CAAAAGTGAA AAGGCGCTGC TAGTCAACCA ATTTGAGCTG 
AGCATCAAGA CTGAAGCCAC TCAGGGTCTC ATCCTGTGGA GCGGGAAAGG GACAGAACGT 
GCCTATTATA TTGCCTTGGC TGTAGTTGGA GGTTATGTTG AGATGACCTA TGACCTGGGC 
TCCAAGCCTG TTGTCCTACG ATCCACAGTA CCTGTGAACA CCAACGAGTG GATTCACATC 
AAAGCTGTAC GAGACAACCG AGATGGCATT TTACGAGTTG GCAATGAGGA CCCCGTCCAT 
GGATCCTCTC CTTTTGGAGT CAAAGAGCTG GACACTGATG GAGCCCTATG GCTGGGTGGA 
ATAGAAAAGC TGAGTGCCTC ATACAAGCTG CCCAAAGCGT ACAGCACGGG ATTCATTGGC 
TGCATGAAGG ACGTTCTGGT GGACCAACAA GACCTGCACT TAGTGGAAGA TGCATTGAAT 
AAACCACCCA TATTGTACTG CTCAGCCAAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agrn ( XM_012967110.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822564.1 Xenopus tropicalis agrin (agrn), transcript variant X4, mRNA.

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CloneID	OXa35299
Clone ID Related Accession (Same CDS sequence)	XM_012967110.2 , XM_012967110.3
Accession Version	XM_012967110.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	agrin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967110.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG ATAAACCTGG CCCTGTCCCT 
AATGCTCAGC CAACTCTACA AGATGTGTGC CGAGGAATGC TCTGCGGATT TGGCGCAGTA 
TGTGAGAAAA GCAGCACCGA CCCATCTCAG GCAACCTGCA TCTGTAAGAA AACCGCCTGC 
GCCTCCATTG TTGCCCCTGT GTGCGGTTCC GACTATTCTA CTTACAGCAA TGAGTGTGAA 
CTGGAGAGAG CCCAGTGCAA CCAGCAGAGG AGAATCAAAG TCATCAGCAA GGGGCCATGC 
GGCACCAAGG ACCCATGCTC AACAGTGTCG TGTAGCTATG GCAGTACCTG TGTCCGCTCA 
ACAGATGGCC AGACGGCAAA GTGTATCTGT CCTGCTACCT GCACAGGGGT TCCAGAAAAT 
GTTGTTTGTG GCAGTGACGG CAAAGATTAT CACAATGAGT GTCAGCTTAA CAAGCAGGCA 
TGCGACAATC AGGAGAATCT CTACAAGAAG TTTGATGGGC GTTGTGATCC ATGCAGAATT 
GCCATGAATA ATCAAAACAA AGCATGTCGA GTAAACCCAC GAACTAGGCA ACTGGAAATG 
CAATACCGAC CAGAATACTG CCCAGTCCGC AGAGATCCTG TTTGCGCAGA TGACGGTGTG 
ACTTATGACA ATGATTGTGT AATGACTCGA TCTGGATTCA TCCGTGGCAT TGAACTGCAG 
AAGGTGCACT CAGGGCAGTG CCAACCGCAA GACAAATGCA AGGAGGACTG TAAACACAAT 
TCTGTGTGTC TTAACCGGAG AGGTGTGACG CGTTGTTCTT GCGACCGTGT TACTTGTGAT 
AGCACTTACA AACCAGTCTG TGGTGGGGAC AGTCGCACGT ATGCCAATGA CTGCGAGAGA 
CAAAAGGCAG AATGCTTTCA GAAAGCTACC ATTCCAGTAA AACACAAAGG CCCATGTGAT 
CTCCATTCCC CCAGTCCATG CCTGAACACA ACCTGCAGTT TTGGAGCAGC ATGTGTAGTA 
AAAAACAAGA AGGCAGTATG CGAATGCCAG CAGGTGTGCC AGGCAGTATA TGACCCAGTG 
TGTGGCAGTG ATAAGCGCAC TTACGGCAAC CCATGTGAAC TGCAGTCAAC AGCCTGCACG 
CTTAAAAAAG AAATAACCAT TTTACACAAG GGGCCTTGTG ACCGCTGTGG GAAATGCCAG 
TTTGGAGCCA TCTGTGAAGC AGAGACTGGC CGCTGTGTAT GTCCAACAGA ATGTGTGCCT 
TCCGCTCAGC CTGTTTGTGG CACTGATGGA AATACATACG AAAGCGAGTG TGAGCTCCAT 
GTACGAGCAT GCACTCAGCA GATTAATCTA CAAGTTGCAG CCCAAGGAGA CTGCAAAACC 
TGTGGCAATA CTCTTTGTAC TTTTGGTGCC AAATGTGTGA ACAACCAGTG TGTGTGCCTA 
ACATGCGAGC GGCAACCCTA CCTACCAGTG TGCGGCAGCA ATGGCGTCAC TTATGATAAC 
CAATGTGAAC TGAAAGCTGC ATCCTGTAAG CAGATGAAGC TGATTGAAGT ATCTCGGGCA 
GGATCTTGTG AAGACGAATG CGGCTCTGGC ACTGACGGTT CTGGTTCTGG TGAGGACAGC 
GAGTGTGAGC AGGATCGATG CAGGCGCTAT GGTGGCTCAT GGGATGAAGA TGCAGAAGAT 
GATCGCTGCG TCTGTGACTT TACCTGTCAT GCTGTGCTTC GAATTTTGGT CTGTGGATCG 
GATGGCATTA CCTACTCTAA TGAGTGTGAG CTAAAGAAAA CTCGGTGTGA GAAACGTCAG 
GATCTGTATG TGGCTGGACA GGGTGCCTGT CGAGCTGCCA CCACCAGCTC TCCTCCTTTG 
GTGCCACACT GCAGTCACAC TGTGTATGGC TGTTGTCCTG ACAATATCAC AGCTGCCCTT 
GGCGTTGGCC TTGCCGGATG CCCAAGCAGC TGTCAGTGCC ACCAGTATGG CTCCTATGGA 
GGAACCTGTG ATCCCACCAC TGGTCAGTGT TCTTGTAAAC CAGGCGTGGG GGGACTGAAA 
TGCGATAGAT GTGAGCCCGG GTTTTGGAAC TTCCGGGGCA TTGTTACGGA TAAGAAAAGC 
GGCTGCACCC CATGTAACTG TGACCCAGTT GGTGCCGTTC GAGATGACTG TGAGCAAATG 
ACAGGACTTT GTTCTTGCAA AACGGGAATC TCAGGTTTGA AGTGCAGCCA GTGCCCTGAT 
GGACGCAAGC CTGGCCTTAC AGGCTGTGAG AAAGATCCTT CAGCTCCTAA ATCTTGTGAT 
GCCATCAAGT GCCAATTTGG TGCAACTTGT GTGGTAATTG GAGGATTTGC TCATTGCGAG 
TGCCCATCTC CCCTGTGTGC TGAGGCGAAT CTGACAAAGG TCTGTGGTTC CGATGATGTG 
ACTTACGGAG ACTGGTGCCA ACTGAAAACC ATTGCTTGCC GTCAGGGCCG CACTATCACA 
GTGAAGCACA CTGGTCCGTG CCAAGAAACT GTCACTCATG CCAACCTTCA GACATTGAGT 
CCAACAGGAA CACCCTCTCC GCCCATAGTT CGGACTCTTC CAACTCCAGG AACCACAAAG 
ATAACAAAGA CCAAGAAAAA GACAGATTCC TTCCAGTTAG TTTTAGAAGC CACATCGCTC 
AGCCCATTTT CCAGCACCAC AATGAGACCT GTGACCCAGA CGCATAGAGC TCACACTTCT 
ACTGTCAGGC CAGCAATCAC TATTCCCACA GTCCATCCCA CTGTGCTTCA GATTTATGAT 
GAATCAGGAA GTGGAAGTGG GGATGAGGAG CTAGAAGCTA GCGGTGATCA AGATTTAAGT 
GGTGTTGAAC CCAAAGAGCC ATTTGATGTG GAGAATGAAC AGAAGGAAGA AGCTGAAGAG 
AAGAGAGATT TTGTAGCCAC CACGGTCATA GAAAGAGCCA GCTGCTATAA CACACCTCTA 
GGATGCTGCT CTGATGGAAA GACCCCAGCT AATGATGCTG AAGGAACAAA TTGCCCAAAA 
ACCAAAATTT TCCAAGGTGT CCTCATTATT GAAGAGTTGG AGGGGCAAGA GGTTTTCTAC 
ACACCAGAAA TGTCAGATTA CAAGTCTGAG CTCTTTGGGG AAACTGCTAG AAGCATTGAG 
AATGCACTGG ATAATCTACT TCGAAATTCA GACATCAAGA GGGATTTCAA GAGCATCCGT 
GTGAGGGACC TTGGACCAAG CAATTCCATC AGAGCCATTG TGGAAGCACA TTTTGATCCA 
GCTACCACCA TGTCTGCTAT GGATGTTCAG AGAGCATTGC TGCGACAGCT CAAGAGCTCC 
AAGAAGAGAG CAATAACCAT AAAGAAGCCT ATTCAGGAAA ATGTCAAGTT CCTGGACTTT 
GGTGAGATCC CTGACTGGGT GCCTCTAATG GTTACAACCA CAACTACAAC GGCAGCAACC 
ACTAGCATGG TAGCTACTAC TGCTGTTGCC CCCACCACCA CAACCCCTGT AGTCGCTACC 
ACAACAACTG TGGCAAGAAC TCCACTACCT AGAAGAATAG TTGCAGTCAA GACCACCCCT 
GTGACAATCA CTCAGCAACC TGTCACCACT CCAAGAATGA CAACAAGACG ACCAGTAACT 
ACAAAAAGAC CAATGACCAC TATTCCTGCC ACCACCAGGA GAAAGCCACC ACGTATTCAA 
GCAGTCACAA AAAAGCCACC CCGGCCATGT GAATCACACC CTTGTCTACA TGGAGGTACT 
TGTGAGGATA ATGGGAAGGA TTTCACCTGT AGCTGCCCAG CTGGCAGAGG AGGAGCTGTG 
TGCGAGAAGA GTATTAAGTA TTTTATTCCT GGGTTTGGTG GTAAGTCATA CCTGGCATTC 
AAGATGATGA AGGCATATCA TACAGTGCGC ATAGCCATGG AGTTCCGATC CTCTGAGCAG 
AACGGGCTTC TCCTATACAA TGGTCAGTCA CTTGGGAAGG ACTTTATCTC ACTTGCAGTT 
AATAATGGCT TTGTGGAACT CAGGTTTAAT ACTGGATCTG GAACTGGAAT AATAACTAGT 
AAAGTGCCCA TTGAACCTGG CAAGTGGCAT CAGTTAGTAG TGAACCGTAA CAGACGCAGT 
GGCATGTTGT CTGTGGATGG TGAATCCCAT GTCAATGGTG AAAGTCCATC TGGAACCGAT 
GGCCTCAACC TAGACACAGA CCTGTTTATA GGAGGAGCTC CTGAGGACCA GATGGCTGTG 
GTATCAGAAA GGACCTCTTT GTCTGTGGGG CTTAAAGGCT GCATCCGACA ACTTGATGTG 
AACAACCAAC TATACAACCT TCAAGAGGGA GGGGCTGATG TCTTGTATGG AAGTGGGGTG 
GGAGAGTGTG GAAACAACCC TTGCCAGCCC AACCCATGCC ATAATGGTGC TGCATGCCAA 
GTAAAGGAGG CTGAAATGTT CCACTGCCAG TGTCTTAGTG GTTATACAGG GCCAACCTGT 
GCAGATGAAC GGAACCCATG TGACCCCAAC CCCTGCCACG TTTCTGCTAC CTGTTTGGTT 
CTACCTGAGG GAGGTGCTAA ATGTGAATGT CCAATGGGCA GAGAGGGAGA GTTTTGTGAG 
AAAGTGAGCA ACCAGGATCT GAATTTGCCA TTCTTACCTC AGTTTAATGG ATACTCTTAC 
TTGGAAATGA AGGGACTACA GACATTTGCC ACTGACCTAC AGGACAAGCT TGCCATGGAA 
GTAGTGTTCC TAGCCAGCAA CCCCAATGGC CTCATCTTCT ATAATGGGCA GAAAACTGAT 
GGAAAAGGAG ATTTTGTGTC ACTGTCCCTT CATGATGGCA ATCTAGAATA CCGTTACGAT 
CTCGGAAAGG GAGCTGCTGT CATCAAGAGT AAGGAAAAGA TTCCACTCAA CACATGGCTC 
AGCGTCTCCC TTGAGCGTAG CGGCAGGAAG GGATTGATGC GGATCAATAA CATGGAACAA 
GTTACTGGGG AATCACCGAA ATCCCGTAAG GCACCACATA CCGCTCTCAA TCTGAAAGAG 
CCTCTATTTG TGGGTGGAGC TCCAGATTTC AGCAAATTAG CCAGGGCAGC GGCTGCCTCT 
ACAAGCTTTG AAGGAGCCAT TCAGAAGATT TCCATTAAGG GGCTGCCTGT TTTGAGAGAA 
GAAAACATCC GCAGTGCCAT TGAGATCTCT ACTTTCCGCA GCCATCCTTG TACCAAGAAA 
CCCTTCCCTT GCCAGAATGG GGGGCTTTGT TCTCCTAATA AAGGCAACTA TGAGTGTGTT 
TGCCAGAGGG GTTTCTCAGG CAGCCAGTGT GAAAAAGTGA CCATTGAGAA AGCAGCTGGA 
GAGTCTGAGG CAGTATCATT TGATGGCAAG ACTTACTTGG AATATCACAT TGCTGTAACC 
AAAAGCCAAC TAACTAACGA GATACCTGAA ACTGGTCCTG TGGACAATCC AGATGACCCC 
AGTGAAAAGG CGCTGCTAGT CAACCAATTT GAGCTGAGCA TCAAGACTGA AGCCACTCAG 
GGTCTCATCC TGTGGAGCGG GAAAGGGACA GAACGTGCCT ATTATATTGC CTTGGCTGTA 
GTTGGAGGTT ATGTTGAGAT GACCTATGAC CTGGGCTCCA AGCCTGTTGT CCTACGATCC 
ACAGTACCTG TGAACACCAA CGAGTGGATT CACATCAAAG CTGTACGAGA CAACCGAGAT 
GGCATTTTAC GAGTTGGCAA TGAGGACCCC GTCCATGGAT CCTCTCCTTT TGGAGTCAAA 
GAGCTGGACA CTGATGGAGC CCTATGGCTG GGTGGAATAG AAAAGCTGAG TGCCTCATAC 
AAGCTGCCCA AAGCGTACAG CACGGGATTC ATTGGCTGCA TGAAGGACGT TCTGGTGGAC 
CAACAAGACC TGCACTTAGT GGAAGATGCA TTGAATAAAC CACCCATATT GTACTGCTCA 
GCCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822564.1
CDS	679..6987
Translation	MGRRWGAPAVLLWTLWALAAVGLGFSRGPCPERELELREEEANVVLTGTVEEIMNVDPVHHTYSCKVRVWRYLKGREIVTNDILLDGGNKVMVGGFGDPTICDNQVSTGDTRIFFVNPAPRSMWPTHRNELMLNSSLMRITLRNLEEVEMCVEDKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACASIVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDPCSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRVNPRTRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQDKCKEDCKHNSVCLNRRGVTRCSCDRVTCDSTYKPVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCSFGAACVVKNKKAVCECQQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGAICEAETGRCVCPTECVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKCVNNQCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETVTHANLQTLSPTGTPSPPIVRTLPTPGTTKITKTKKKTDSFQLVLEATSLSPFSSTTMRPVTQTHRAHTSTVRPAITIPTVHPTVLQIYDESGSGSGDEELEASGDQDLSGVEPKEPFDVENEQKEEAEEKRDFVATTVIERASCYNTPLGCCSDGKTPANDAEGTNCPKTKIFQGVLIIEELEGQEVFYTPEMSDYKSELFGETARSIENALDNLLRNSDIKRDFKSIRVRDLGPSNSIRAIVEAHFDPATTMSAMDVQRALLRQLKSSKKRAITIKKPIQENVKFLDFGEIPDWVPLMVTTTTTTAATTSMVATTAVAPTTTTPVVATTTTVARTPLPRRIVAVKTTPVTITQQPVTTPRMTTRRPVTTKRPMTTIPATTRRKPPRIQAVTKKPPRPCESHPCLHGGTCEDNGKDFTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNLKEPLFVGGAPDFSKLARAAAASTSFEGAIQKISIKGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLCSPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSQLTNEIPETGPVDNPDDPSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKELDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDALNKPPILYCSAK

Target ORF information:

RefSeq Version	XM_012967110.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis agrin (agrn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967110.2

ORF Insert Sequence:

ATGGGAAGAA GGTGGGGAGC TCCGGCCGTG CTTCTTTGGA CTTTATGGGC TCTGGCGGCG 
GTAGGTTTGG GGTTCAGCAG GGGCCCGTGT CCCGAGAGGG AGCTGGAGCT GAGGGAAGAG 
GAGGCGAACG TTGTGCTCAC CGGGACGGTG GAGGAGATCA TGAACGTGGA CCCGGTGCAC 
CACACCTACT CCTGCAAGGT TAGAGTATGG CGATATCTGA AGGGCCGAGA GATTGTGACA 
AATGATATTC TGCTGGATGG AGGGAACAAA GTAATGGTTG GAGGATTTGG AGATCCCACA 
ATCTGTGACA ATCAGGTGTC TACAGGGGAC ACAAGGATTT TCTTTGTTAA TCCCGCCCCC 
CGCTCTATGT GGCCCACTCA TCGTAATGAG CTGATGCTAA ACTCCAGCCT AATGAGGATA 
ACCCTTAGGA ATCTGGAAGA GGTAGAAATG TGTGTTGAAG ATAAACCTGG CCCTGTCCCT 
AATGCTCAGC CAACTCTACA AGATGTGTGC CGAGGAATGC TCTGCGGATT TGGCGCAGTA 
TGTGAGAAAA GCAGCACCGA CCCATCTCAG GCAACCTGCA TCTGTAAGAA AACCGCCTGC 
GCCTCCATTG TTGCCCCTGT GTGCGGTTCC GACTATTCTA CTTACAGCAA TGAGTGTGAA 
CTGGAGAGAG CCCAGTGCAA CCAGCAGAGG AGAATCAAAG TCATCAGCAA GGGGCCATGC 
GGCACCAAGG ACCCATGCTC AACAGTGTCG TGTAGCTATG GCAGTACCTG TGTCCGCTCA 
ACAGATGGCC AGACGGCAAA GTGTATCTGT CCTGCTACCT GCACAGGGGT TCCAGAAAAT 
GTTGTTTGTG GCAGTGACGG CAAAGATTAT CACAATGAGT GTCAGCTTAA CAAGCAGGCA 
TGCGACAATC AGGAGAATCT CTACAAGAAG TTTGATGGGC GTTGTGATCC ATGCAGAATT 
GCCATGAATA ATCAAAACAA AGCATGTCGA GTAAACCCAC GAACTAGGCA ACTGGAAATG 
CAATACCGAC CAGAATACTG CCCAGTCCGC AGAGATCCTG TTTGCGCAGA TGACGGTGTG 
ACTTATGACA ATGATTGTGT AATGACTCGA TCTGGATTCA TCCGTGGCAT TGAACTGCAG 
AAGGTGCACT CAGGGCAGTG CCAACCGCAA GACAAATGCA AGGAGGACTG TAAACACAAT 
TCTGTGTGTC TTAACCGGAG AGGTGTGACG CGTTGTTCTT GCGACCGTGT TACTTGTGAT 
AGCACTTACA AACCAGTCTG TGGTGGGGAC AGTCGCACGT ATGCCAATGA CTGCGAGAGA 
CAAAAGGCAG AATGCTTTCA GAAAGCTACC ATTCCAGTAA AACACAAAGG CCCATGTGAT 
CTCCATTCCC CCAGTCCATG CCTGAACACA ACCTGCAGTT TTGGAGCAGC ATGTGTAGTA 
AAAAACAAGA AGGCAGTATG CGAATGCCAG CAGGTGTGCC AGGCAGTATA TGACCCAGTG 
TGTGGCAGTG ATAAGCGCAC TTACGGCAAC CCATGTGAAC TGCAGTCAAC AGCCTGCACG 
CTTAAAAAAG AAATAACCAT TTTACACAAG GGGCCTTGTG ACCGCTGTGG GAAATGCCAG 
TTTGGAGCCA TCTGTGAAGC AGAGACTGGC CGCTGTGTAT GTCCAACAGA ATGTGTGCCT 
TCCGCTCAGC CTGTTTGTGG CACTGATGGA AATACATACG AAAGCGAGTG TGAGCTCCAT 
GTACGAGCAT GCACTCAGCA GATTAATCTA CAAGTTGCAG CCCAAGGAGA CTGCAAAACC 
TGTGGCAATA CTCTTTGTAC TTTTGGTGCC AAATGTGTGA ACAACCAGTG TGTGTGCCTA 
ACATGCGAGC GGCAACCCTA CCTACCAGTG TGCGGCAGCA ATGGCGTCAC TTATGATAAC 
CAATGTGAAC TGAAAGCTGC ATCCTGTAAG CAGATGAAGC TGATTGAAGT ATCTCGGGCA 
GGATCTTGTG AAGACGAATG CGGCTCTGGC ACTGACGGTT CTGGTTCTGG TGAGGACAGC 
GAGTGTGAGC AGGATCGATG CAGGCGCTAT GGTGGCTCAT GGGATGAAGA TGCAGAAGAT 
GATCGCTGCG TCTGTGACTT TACCTGTCAT GCTGTGCTTC GAATTTTGGT CTGTGGATCG 
GATGGCATTA CCTACTCTAA TGAGTGTGAG CTAAAGAAAA CTCGGTGTGA GAAACGTCAG 
GATCTGTATG TGGCTGGACA GGGTGCCTGT CGAGCTGCCA CCACCAGCTC TCCTCCTTTG 
GTGCCACACT GCAGTCACAC TGTGTATGGC TGTTGTCCTG ACAATATCAC AGCTGCCCTT 
GGCGTTGGCC TTGCCGGATG CCCAAGCAGC TGTCAGTGCC ACCAGTATGG CTCCTATGGA 
GGAACCTGTG ATCCCACCAC TGGTCAGTGT TCTTGTAAAC CAGGCGTGGG GGGACTGAAA 
TGCGATAGAT GTGAGCCCGG GTTTTGGAAC TTCCGGGGCA TTGTTACGGA TAAGAAAAGC 
GGCTGCACCC CATGTAACTG TGACCCAGTT GGTGCCGTTC GAGATGACTG TGAGCAAATG 
ACAGGACTTT GTTCTTGCAA AACGGGAATC TCAGGTTTGA AGTGCAGCCA GTGCCCTGAT 
GGACGCAAGC CTGGCCTTAC AGGCTGTGAG AAAGATCCTT CAGCTCCTAA ATCTTGTGAT 
GCCATCAAGT GCCAATTTGG TGCAACTTGT GTGGTAATTG GAGGATTTGC TCATTGCGAG 
TGCCCATCTC CCCTGTGTGC TGAGGCGAAT CTGACAAAGG TCTGTGGTTC CGATGATGTG 
ACTTACGGAG ACTGGTGCCA ACTGAAAACC ATTGCTTGCC GTCAGGGCCG CACTATCACA 
GTGAAGCACA CTGGTCCGTG CCAAGAAACT GTCACTCATG CCAACCTTCA GACATTGAGT 
CCAACAGGAA CACCCTCTCC GCCCATAGTT CGGACTCTTC CAACTCCAGG AACCACAAAG 
ATAACAAAGA CCAAGAAAAA GACAGATTCC TTCCAGTTAG TTTTAGAAGC CACATCGCTC 
AGCCCATTTT CCAGCACCAC AATGAGACCT GTGACCCAGA CGCATAGAGC TCACACTTCT 
ACTGTCAGGC CAGCAATCAC TATTCCCACA GTCCATCCCA CTGTGCTTCA GATTTATGAT 
GAATCAGGAA GTGGAAGTGG GGATGAGGAG CTAGAAGCTA GCGGTGATCA AGATTTAAGT 
GGTGTTGAAC CCAAAGAGCC ATTTGATGTG GAGAATGAAC AGAAGGAAGA AGCTGAAGAG 
AAGAGAGATT TTGTAGCCAC CACGGTCATA GAAAGAGCCA GCTGCTATAA CACACCTCTA 
GGATGCTGCT CTGATGGAAA GACCCCAGCT AATGATGCTG AAGGAACAAA TTGCCCAAAA 
ACCAAAATTT TCCAAGGTGT CCTCATTATT GAAGAGTTGG AGGGGCAAGA GGTTTTCTAC 
ACACCAGAAA TGTCAGATTA CAAGTCTGAG CTCTTTGGGG AAACTGCTAG AAGCATTGAG 
AATGCACTGG ATAATCTACT TCGAAATTCA GACATCAAGA GGGATTTCAA GAGCATCCGT 
GTGAGGGACC TTGGACCAAG CAATTCCATC AGAGCCATTG TGGAAGCACA TTTTGATCCA 
GCTACCACCA TGTCTGCTAT GGATGTTCAG AGAGCATTGC TGCGACAGCT CAAGAGCTCC 
AAGAAGAGAG CAATAACCAT AAAGAAGCCT ATTCAGGAAA ATGTCAAGTT CCTGGACTTT 
GGTGAGATCC CTGACTGGGT GCCTCTAATG GTTACAACCA CAACTACAAC GGCAGCAACC 
ACTAGCATGG TAGCTACTAC TGCTGTTGCC CCCACCACCA CAACCCCTGT AGTCGCTACC 
ACAACAACTG TGGCAAGAAC TCCACTACCT AGAAGAATAG TTGCAGTCAA GACCACCCCT 
GTGACAATCA CTCAGCAACC TGTCACCACT CCAAGAATGA CAACAAGACG ACCAGTAACT 
ACAAAAAGAC CAATGACCAC TATTCCTGCC ACCACCAGGA GAAAGCCACC ACGTATTCAA 
GCAGTCACAA AAAAGCCACC CCGGCCATGT GAATCACACC CTTGTCTACA TGGAGGTACT 
TGTGAGGATA ATGGGAAGGA TTTCACCTGT AGCTGCCCAG CTGGCAGAGG AGGAGCTGTG 
TGCGAGAAGA GTATTAAGTA TTTTATTCCT GGGTTTGGTG GTAAGTCATA CCTGGCATTC 
AAGATGATGA AGGCATATCA TACAGTGCGC ATAGCCATGG AGTTCCGATC CTCTGAGCAG 
AACGGGCTTC TCCTATACAA TGGTCAGTCA CTTGGGAAGG ACTTTATCTC ACTTGCAGTT 
AATAATGGCT TTGTGGAACT CAGGTTTAAT ACTGGATCTG GAACTGGAAT AATAACTAGT 
AAAGTGCCCA TTGAACCTGG CAAGTGGCAT CAGTTAGTAG TGAACCGTAA CAGACGCAGT 
GGCATGTTGT CTGTGGATGG TGAATCCCAT GTCAATGGTG AAAGTCCATC TGGAACCGAT 
GGCCTCAACC TAGACACAGA CCTGTTTATA GGAGGAGCTC CTGAGGACCA GATGGCTGTG 
GTATCAGAAA GGACCTCTTT GTCTGTGGGG CTTAAAGGCT GCATCCGACA ACTTGATGTG 
AACAACCAAC TATACAACCT TCAAGAGGGA GGGGCTGATG TCTTGTATGG AAGTGGGGTG 
GGAGAGTGTG GAAACAACCC TTGCCAGCCC AACCCATGCC ATAATGGTGC TGCATGCCAA 
GTAAAGGAGG CTGAAATGTT CCACTGCCAG TGTCTTAGTG GTTATACAGG GCCAACCTGT 
GCAGATGAAC GGAACCCATG TGACCCCAAC CCCTGCCACG TTTCTGCTAC CTGTTTGGTT 
CTACCTGAGG GAGGTGCTAA ATGTGAATGT CCAATGGGCA GAGAGGGAGA GTTTTGTGAG 
AAAGTGAGCA ACCAGGATCT GAATTTGCCA TTCTTACCTC AGTTTAATGG ATACTCTTAC 
TTGGAAATGA AGGGACTACA GACATTTGCC ACTGACCTAC AGGACAAGCT TGCCATGGAA 
GTAGTGTTCC TAGCCAGCAA CCCCAATGGC CTCATCTTCT ATAATGGGCA GAAAACTGAT 
GGAAAAGGAG ATTTTGTGTC ACTGTCCCTT CATGATGGCA ATCTAGAATA CCGTTACGAT 
CTCGGAAAGG GAGCTGCTGT CATCAAGAGT AAGGAAAAGA TTCCACTCAA CACATGGCTC 
AGCGTCTCCC TTGAGCGTAG CGGCAGGAAG GGATTGATGC GGATCAATAA CATGGAACAA 
GTTACTGGGG AATCACCGAA ATCCCGTAAG GCACCACATA CCGCTCTCAA TCTGAAAGAG 
CCTCTATTTG TGGGTGGAGC TCCAGATTTC AGCAAATTAG CCAGGGCAGC GGCTGCCTCT 
ACAAGCTTTG AAGGAGCCAT TCAGAAGATT TCCATTAAGG GGCTGCCTGT TTTGAGAGAA 
GAAAACATCC GCAGTGCCAT TGAGATCTCT ACTTTCCGCA GCCATCCTTG TACCAAGAAA 
CCCTTCCCTT GCCAGAATGG GGGGCTTTGT TCTCCTAATA AAGGCAACTA TGAGTGTGTT 
TGCCAGAGGG GTTTCTCAGG CAGCCAGTGT GAAAAAGTGA CCATTGAGAA AGCAGCTGGA 
GAGTCTGAGG CAGTATCATT TGATGGCAAG ACTTACTTGG AATATCACAT TGCTGTAACC 
AAAAGCCAAC TAACTAACGA GATACCTGAA ACTGGTCCTG TGGACAATCC AGATGACCCC 
AGTGAAAAGG CGCTGCTAGT CAACCAATTT GAGCTGAGCA TCAAGACTGA AGCCACTCAG 
GGTCTCATCC TGTGGAGCGG GAAAGGGACA GAACGTGCCT ATTATATTGC CTTGGCTGTA 
GTTGGAGGTT ATGTTGAGAT GACCTATGAC CTGGGCTCCA AGCCTGTTGT CCTACGATCC 
ACAGTACCTG TGAACACCAA CGAGTGGATT CACATCAAAG CTGTACGAGA CAACCGAGAT 
GGCATTTTAC GAGTTGGCAA TGAGGACCCC GTCCATGGAT CCTCTCCTTT TGGAGTCAAA 
GAGCTGGACA CTGATGGAGC CCTATGGCTG GGTGGAATAG AAAAGCTGAG TGCCTCATAC 
AAGCTGCCCA AAGCGTACAG CACGGGATTC ATTGGCTGCA TGAAGGACGT TCTGGTGGAC 
CAACAAGACC TGCACTTAGT GGAAGATGCA TTGAATAAAC CACCCATATT GTACTGCTCA 
GCCAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

agrn ( XM_012967115.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822569.1 Xenopus tropicalis agrin (agrn), transcript variant X7, mRNA.

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CloneID	OXa35304
Clone ID Related Accession (Same CDS sequence)	XM_012967115.2 , XM_012967115.3
Accession Version	XM_012967115.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	agrin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967115.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGCT GCCACTACCC TGCTCCAGGT TTGGAGAGGG ACCGGATCTA TCAGCATAAA 
GTCTCACTGG TTGTGCGCTA CTTCATGATC CCTTGTAACA TATGCCTGAT CCTGCTTGCC 
ACTTCCACGC TTGGATTTGC CGTCCTGCTG TTCCTGAATA ACTATAAACC TGGCCCTGTC 
CCTAATGCTC AGCCAACTCT ACAAGATGTG TGCCGAGGAA TGCTCTGCGG ATTTGGCGCA 
GTATGTGAGA AAAGCAGCAC CGACCCATCT CAGGCAACCT GCATCTGTAA GAAAACCGCC 
TGCGCCTCCA TTGTTGCCCC TGTGTGCGGT TCCGACTATT CTACTTACAG CAATGAGTGT 
GAACTGGAGA GAGCCCAGTG CAACCAGCAG AGGAGAATCA AAGTCATCAG CAAGGGGCCA 
TGCGGCACCA AGGACCCATG CTCAACAGTG TCGTGTAGCT ATGGCAGTAC CTGTGTCCGC 
TCAACAGATG GCCAGACGGC AAAGTGTATC TGTCCTGCTA CCTGCACAGG GGTTCCAGAA 
AATGTTGTTT GTGGCAGTGA CGGCAAAGAT TATCACAATG AGTGTCAGCT TAACAAGCAG 
GCATGCGACA ATCAGGAGAA TCTCTACAAG AAGTTTGATG GGCGTTGTGA TCCATGCAGA 
ATTGCCATGA ATAATCAAAA CAAAGCATGT CGAGTAAACC CACGAACTAG GCAACTGGAA 
ATGCAATACC GACCAGAATA CTGCCCAGTC CGCAGAGATC CTGTTTGCGC AGATGACGGT 
GTGACTTATG ACAATGATTG TGTAATGACT CGATCTGGAT TCATCCGTGG CATTGAACTG 
CAGAAGGTGC ACTCAGGGCA GTGCCAACCG CAAGACAAAT GCAAGGAGGA CTGTAAACAC 
AATTCTGTGT GTCTTAACCG GAGAGGTGTG ACGCGTTGTT CTTGCGACCG TGTTACTTGT 
GATAGCACTT ACAAACCAGT CTGTGGTGGG GACAGTCGCA CGTATGCCAA TGACTGCGAG 
AGACAAAAGG CAGAATGCTT TCAGAAAGCT ACCATTCCAG TAAAACACAA AGGCCCATGT 
GATCTCCATT CCCCCAGTCC ATGCCTGAAC ACAACCTGCA GTTTTGGAGC AGCATGTGTA 
GTAAAAAACA AGAAGGCAGT ATGCGAATGC CAGCAGGTGT GCCAGGCAGT ATATGACCCA 
GTGTGTGGCA GTGATAAGCG CACTTACGGC AACCCATGTG AACTGCAGTC AACAGCCTGC 
ACGCTTAAAA AAGAAATAAC CATTTTACAC AAGGGGCCTT GTGACCGCTG TGGGAAATGC 
CAGTTTGGAG CCATCTGTGA AGCAGAGACT GGCCGCTGTG TATGTCCAAC AGAATGTGTG 
CCTTCCGCTC AGCCTGTTTG TGGCACTGAT GGAAATACAT ACGAAAGCGA GTGTGAGCTC 
CATGTACGAG CATGCACTCA GCAGATTAAT CTACAAGTTG CAGCCCAAGG AGACTGCAAA 
ACCTGTGGCA ATACTCTTTG TACTTTTGGT GCCAAATGTG TGAACAACCA GTGTGTGTGC 
CTAACATGCG AGCGGCAACC CTACCTACCA GTGTGCGGCA GCAATGGCGT CACTTATGAT 
AACCAATGTG AACTGAAAGC TGCATCCTGT AAGCAGATGA AGCTGATTGA AGTATCTCGG 
GCAGGATCTT GTGAAGACGA ATGCGGCTCT GGCACTGACG GTTCTGGTTC TGGTGAGGAC 
AGCGAGTGTG AGCAGGATCG ATGCAGGCGC TATGGTGGCT CATGGGATGA AGATGCAGAA 
GATGATCGCT GCGTCTGTGA CTTTACCTGT CATGCTGTGC TTCGAATTTT GGTCTGTGGA 
TCGGATGGCA TTACCTACTC TAATGAGTGT GAGCTAAAGA AAACTCGGTG TGAGAAACGT 
CAGGATCTGT ATGTGGCTGG ACAGGGTGCC TGTCGAGCTG CCACCACCAG CTCTCCTCCT 
TTGGTGCCAC ACTGCAGTCA CACTGTGTAT GGCTGTTGTC CTGACAATAT CACAGCTGCC 
CTTGGCGTTG GCCTTGCCGG ATGCCCAAGC AGCTGTCAGT GCCACCAGTA TGGCTCCTAT 
GGAGGAACCT GTGATCCCAC CACTGGTCAG TGTTCTTGTA AACCAGGCGT GGGGGGACTG 
AAATGCGATA GATGTGAGCC CGGGTTTTGG AACTTCCGGG GCATTGTTAC GGATAAGAAA 
AGCGGCTGCA CCCCATGTAA CTGTGACCCA GTTGGTGCCG TTCGAGATGA CTGTGAGCAA 
ATGACAGGAC TTTGTTCTTG CAAAACGGGA ATCTCAGGTT TGAAGTGCAG CCAGTGCCCT 
GATGGACGCA AGCCTGGCCT TACAGGCTGT GAGAAAGATC CTTCAGCTCC TAAATCTTGT 
GATGCCATCA AGTGCCAATT TGGTGCAACT TGTGTGGTAA TTGGAGGATT TGCTCATTGC 
GAGTGCCCAT CTCCCCTGTG TGCTGAGGCG AATCTGACAA AGGTCTGTGG TTCCGATGAT 
GTGACTTACG GAGACTGGTG CCAACTGAAA ACCATTGCTT GCCGTCAGGG CCGCACTATC 
ACAGTGAAGC ACACTGGTCC GTGCCAAGAA ACTGTCACTC ATGCCAACCT TCAGACATTG 
AGTCCAACAG GAACACCCTC TCCGCCCATA GTTCGGACTC TTCCAACTCC AGGAACCACA 
AAGATAACAA AGACCAAGAA AAAGACAGAT TCCTTCCAGT TAGTTTTAGA AGCCACATCG 
CTCAGCCCAT TTTCCAGCAC CACAATGAGA CCTGTGACCC AGACGCATAG AGCTCACACT 
TCTACTGTCA GGCCAGCAAT CACTATTCCC ACAGTCCATC CCACTGTGCT TCAGATTTAT 
GATGAATCAG GAAGTGGAAG TGGGGATGAG GAGCTAGAAG CTAGCGGTGA TCAAGATTTA 
AGTGGTGTTG AACCCAAAGA GCCATTTGAT GTGGAGAATG AACAGAAGGA AGAAGCTGAA 
GAGAAGAGAG ATTTTGTAGC CACCACGGTC ATAGAAAGAG CCAGCTGCTA TAACACACCT 
CTAGGATGCT GCTCTGATGG AAAGACCCCA GCTAATGATG CTGAAGGAAC AAATTGCCCA 
AAAACCAAAA TTTTCCAAGG TGTCCTCATT ATTGAAGAGT TGGAGGGGCA AGAGGTTTTC 
TACACACCAG AAATGTCAGA TTACAAGTCT GAGCTCTTTG GGGAAACTGC TAGAAGCATT 
GAGAATGCAC TGGATAATCT ACTTCGAAAT TCAGACATCA AGAGGGATTT CAAGAGCATC 
CGTGTGAGGG ACCTTGGACC AAGCAATTCC ATCAGAGCCA TTGTGGAAGC ACATTTTGAT 
CCAGCTACCA CCATGTCTGC TATGGATGTT CAGAGAGCAT TGCTGCGACA GCTCAAGAGC 
TCCAAGAAGA GAGCAATAAC CATAAAGAAG CCTATTCAGG AAAATGTCAA GTTCCTGGAC 
TTTGGTGAGA TCCCTGACTG GGTGCCTCTA ATGGTTACAA CCACAACTAC AACGGCAGCA 
ACCACTAGCA TGGTAGCTAC TACTGCTGTT GCCCCCACCA CCACAACCCC TGTAGTCGCT 
ACCACAACAA CTGTGGCAAG AACTCCACTA CCTAGAAGAA TAGTTGCAGT CAAGACCACC 
CCTGTGACAA TCACTCAGCA ACCTGTCACC ACTCCAAGAA TGACAACAAG ACGACCAGTA 
ACTACAAAAA GACCAATGAC CACTATTCCT GCCACCACCA GGAGAAAGCC ACCACGTATT 
CAAGCAGTCA CAAAAAAGCC ACCCCGGCCA TGTGAATCAC ACCCTTGTCT ACATGGAGGT 
ACTTGTGAGG ATAATGGGAA GGATTTCACC TGTAGCTGCC CAGCTGGCAG AGGAGGAGCT 
GTGTGCGAGA AGAGTATTAA GTATTTTATT CCTGGGTTTG GTGGTAAGTC ATACCTGGCA 
TTCAAGATGA TGAAGGCATA TCATACAGTG CGCATAGCCA TGGAGTTCCG ATCCTCTGAG 
CAGAACGGGC TTCTCCTATA CAATGGTCAG TCACTTGGGA AGGACTTTAT CTCACTTGCA 
GTTAATAATG GCTTTGTGGA ACTCAGGTTT AATACTGGAT CTGGAACTGG AATAATAACT 
AGTAAAGTGC CCATTGAACC TGGCAAGTGG CATCAGTTAG TAGTGAACCG TAACAGACGC 
AGTGGCATGT TGTCTGTGGA TGGTGAATCC CATGTCAATG GTGAAAGTCC ATCTGGAACC 
GATGGCCTCA ACCTAGACAC AGACCTGTTT ATAGGAGGAG CTCCTGAGGA CCAGATGGCT 
GTGGTATCAG AAAGGACCTC TTTGTCTGTG GGGCTTAAAG GCTGCATCCG ACAACTTGAT 
GTGAACAACC AACTATACAA CCTTCAAGAG GGAGGGGCTG ATGTCTTGTA TGGAAGTGGG 
GTGGGAGAGT GTGGAAACAA CCCTTGCCAG CCCAACCCAT GCCATAATGG TGCTGCATGC 
CAAGTAAAGG AGGCTGAAAT GTTCCACTGC CAGTGTCTTA GTGGTTATAC AGGGCCAACC 
TGTGCAGATG AACGGAACCC ATGTGACCCC AACCCCTGCC ACGTTTCTGC TACCTGTTTG 
GTTCTACCTG AGGGAGGTGC TAAATGTGAA TGTCCAATGG GCAGAGAGGG AGAGTTTTGT 
GAGAAAGTGA GCAACCAGGA TCTGAATTTG CCATTCTTAC CTCAGTTTAA TGGATACTCT 
TACTTGGAAA TGAAGGGACT ACAGACATTT GCCACTGACC TACAGGACAA GCTTGCCATG 
GAAGTAGTGT TCCTAGCCAG CAACCCCAAT GGCCTCATCT TCTATAATGG GCAGAAAACT 
GATGGAAAAG GAGATTTTGT GTCACTGTCC CTTCATGATG GCAATCTAGA ATACCGTTAC 
GATCTCGGAA AGGGAGCTGC TGTCATCAAG AGTAAGGAAA AGATTCCACT CAACACATGG 
CTCAGCGTCT CCCTTGAGCG TAGCGGCAGG AAGGGATTGA TGCGGATCAA TAACATGGAA 
CAAGTTACTG GGGAATCACC GAAATCCCGT AAGGCACCAC ATACCGCTCT CAATCTGAAA 
GAGCCTCTAT TTGTGGGTGG AGCTCCAGAT TTCAGCAAAT TAGCCAGGGC AGCGGCTGCC 
TCTACAAGCT TTGAAGGAGC CATTCAGAAG ATTTCCATTA AGGGGCTGCC TGTTTTGAGA 
GAAGAAAACA TCCGCAGTGC CATTGAGATC TCTACTTTCC GCAGCCATCC TTGTACCAAG 
AAACCCTTCC CTTGCCAGAA TGGGGGGCTT TGTTCTCCTA ATAAAGGCAA CTATGAGTGT 
GTTTGCCAGA GGGGTTTCTC AGGCAGCCAG TGTGAAAAAG TGACCATTGA GAAAGCAGCT 
GGAGAGTCTG AGGCAGTATC ATTTGATGGC AAGACTTACT TGGAATATCA CATTGCTGTA 
ACCAAAAGCC AACTAACTAA CGAGATACCT GAAACTGGTC CTGTGGACAA TCCAGATGAC 
CCCAGTGAAA AGGCGCTGCT AGTCAACCAA TTTGAGCTGA GCATCAAGAC TGAAGCCACT 
CAGGGTCTCA TCCTGTGGAG CGGGAAAGGG ACAGAACGTG CCTATTATAT TGCCTTGGCT 
GTAGTTGGAG GTTATGTTGA GATGACCTAT GACCTGGGCT CCAAGCCTGT TGTCCTACGA 
TCCACAGTAC CTGTGAACAC CAACGAGTGG ATTCACATCA AAGCTGTACG AGACAACCGA 
GATGGCATTT TACGAGTTGG CAATGAGGAC CCCGTCCATG GATCCTCTCC TTTTGGAGTC 
AAAGAGCTGG ACACTGATGG AGCCCTATGG CTGGGTGGAA TAGAAAAGCT GAGTGCCTCA 
TACAAGCTGC CCAAAGCGTA CAGCACGGGA TTCATTGGCT GCATGAAGGA CGTTCTGGTG 
GACCAACAAG ACCTGCACTT AGTGGAAGAT GCATTGAATA AACCACCCAT ATTGTACTGC 
TCAGCCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822569.1
CDS	387..6398
Translation	MTGCHYPAPGLERDRIYQHKVSLVVRYFMIPCNICLILLATSTLGFAVLLFLNNYKPGPVPNAQPTLQDVCRGMLCGFGAVCEKSSTDPSQATCICKKTACASIVAPVCGSDYSTYSNECELERAQCNQQRRIKVISKGPCGTKDPCSTVSCSYGSTCVRSTDGQTAKCICPATCTGVPENVVCGSDGKDYHNECQLNKQACDNQENLYKKFDGRCDPCRIAMNNQNKACRVNPRTRQLEMQYRPEYCPVRRDPVCADDGVTYDNDCVMTRSGFIRGIELQKVHSGQCQPQDKCKEDCKHNSVCLNRRGVTRCSCDRVTCDSTYKPVCGGDSRTYANDCERQKAECFQKATIPVKHKGPCDLHSPSPCLNTTCSFGAACVVKNKKAVCECQQVCQAVYDPVCGSDKRTYGNPCELQSTACTLKKEITILHKGPCDRCGKCQFGAICEAETGRCVCPTECVPSAQPVCGTDGNTYESECELHVRACTQQINLQVAAQGDCKTCGNTLCTFGAKCVNNQCVCLTCERQPYLPVCGSNGVTYDNQCELKAASCKQMKLIEVSRAGSCEDECGSGTDGSGSGEDSECEQDRCRRYGGSWDEDAEDDRCVCDFTCHAVLRILVCGSDGITYSNECELKKTRCEKRQDLYVAGQGACRAATTSSPPLVPHCSHTVYGCCPDNITAALGVGLAGCPSSCQCHQYGSYGGTCDPTTGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDKKSGCTPCNCDPVGAVRDDCEQMTGLCSCKTGISGLKCSQCPDGRKPGLTGCEKDPSAPKSCDAIKCQFGATCVVIGGFAHCECPSPLCAEANLTKVCGSDDVTYGDWCQLKTIACRQGRTITVKHTGPCQETVTHANLQTLSPTGTPSPPIVRTLPTPGTTKITKTKKKTDSFQLVLEATSLSPFSSTTMRPVTQTHRAHTSTVRPAITIPTVHPTVLQIYDESGSGSGDEELEASGDQDLSGVEPKEPFDVENEQKEEAEEKRDFVATTVIERASCYNTPLGCCSDGKTPANDAEGTNCPKTKIFQGVLIIEELEGQEVFYTPEMSDYKSELFGETARSIENALDNLLRNSDIKRDFKSIRVRDLGPSNSIRAIVEAHFDPATTMSAMDVQRALLRQLKSSKKRAITIKKPIQENVKFLDFGEIPDWVPLMVTTTTTTAATTSMVATTAVAPTTTTPVVATTTTVARTPLPRRIVAVKTTPVTITQQPVTTPRMTTRRPVTTKRPMTTIPATTRRKPPRIQAVTKKPPRPCESHPCLHGGTCEDNGKDFTCSCPAGRGGAVCEKSIKYFIPGFGGKSYLAFKMMKAYHTVRIAMEFRSSEQNGLLLYNGQSLGKDFISLAVNNGFVELRFNTGSGTGIITSKVPIEPGKWHQLVVNRNRRSGMLSVDGESHVNGESPSGTDGLNLDTDLFIGGAPEDQMAVVSERTSLSVGLKGCIRQLDVNNQLYNLQEGGADVLYGSGVGECGNNPCQPNPCHNGAACQVKEAEMFHCQCLSGYTGPTCADERNPCDPNPCHVSATCLVLPEGGAKCECPMGREGEFCEKVSNQDLNLPFLPQFNGYSYLEMKGLQTFATDLQDKLAMEVVFLASNPNGLIFYNGQKTDGKGDFVSLSLHDGNLEYRYDLGKGAAVIKSKEKIPLNTWLSVSLERSGRKGLMRINNMEQVTGESPKSRKAPHTALNLKEPLFVGGAPDFSKLARAAAASTSFEGAIQKISIKGLPVLREENIRSAIEISTFRSHPCTKKPFPCQNGGLCSPNKGNYECVCQRGFSGSQCEKVTIEKAAGESEAVSFDGKTYLEYHIAVTKSQLTNEIPETGPVDNPDDPSEKALLVNQFELSIKTEATQGLILWSGKGTERAYYIALAVVGGYVEMTYDLGSKPVVLRSTVPVNTNEWIHIKAVRDNRDGILRVGNEDPVHGSSPFGVKELDTDGALWLGGIEKLSASYKLPKAYSTGFIGCMKDVLVDQQDLHLVEDALNKPPILYCSAK

Target ORF information:

RefSeq Version	XM_012967115.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis agrin (agrn), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967115.2

ORF Insert Sequence:

ATGACTGGCT GCCACTACCC TGCTCCAGGT TTGGAGAGGG ACCGGATCTA TCAGCATAAA 
GTCTCACTGG TTGTGCGCTA CTTCATGATC CCTTGTAACA TATGCCTGAT CCTGCTTGCC 
ACTTCCACGC TTGGATTTGC CGTCCTGCTG TTCCTGAATA ACTATAAACC TGGCCCTGTC 
CCTAATGCTC AGCCAACTCT ACAAGATGTG TGCCGAGGAA TGCTCTGCGG ATTTGGCGCA 
GTATGTGAGA AAAGCAGCAC CGACCCATCT CAGGCAACCT GCATCTGTAA GAAAACCGCC 
TGCGCCTCCA TTGTTGCCCC TGTGTGCGGT TCCGACTATT CTACTTACAG CAATGAGTGT 
GAACTGGAGA GAGCCCAGTG CAACCAGCAG AGGAGAATCA AAGTCATCAG CAAGGGGCCA 
TGCGGCACCA AGGACCCATG CTCAACAGTG TCGTGTAGCT ATGGCAGTAC CTGTGTCCGC 
TCAACAGATG GCCAGACGGC AAAGTGTATC TGTCCTGCTA CCTGCACAGG GGTTCCAGAA 
AATGTTGTTT GTGGCAGTGA CGGCAAAGAT TATCACAATG AGTGTCAGCT TAACAAGCAG 
GCATGCGACA ATCAGGAGAA TCTCTACAAG AAGTTTGATG GGCGTTGTGA TCCATGCAGA 
ATTGCCATGA ATAATCAAAA CAAAGCATGT CGAGTAAACC CACGAACTAG GCAACTGGAA 
ATGCAATACC GACCAGAATA CTGCCCAGTC CGCAGAGATC CTGTTTGCGC AGATGACGGT 
GTGACTTATG ACAATGATTG TGTAATGACT CGATCTGGAT TCATCCGTGG CATTGAACTG 
CAGAAGGTGC ACTCAGGGCA GTGCCAACCG CAAGACAAAT GCAAGGAGGA CTGTAAACAC 
AATTCTGTGT GTCTTAACCG GAGAGGTGTG ACGCGTTGTT CTTGCGACCG TGTTACTTGT 
GATAGCACTT ACAAACCAGT CTGTGGTGGG GACAGTCGCA CGTATGCCAA TGACTGCGAG 
AGACAAAAGG CAGAATGCTT TCAGAAAGCT ACCATTCCAG TAAAACACAA AGGCCCATGT 
GATCTCCATT CCCCCAGTCC ATGCCTGAAC ACAACCTGCA GTTTTGGAGC AGCATGTGTA 
GTAAAAAACA AGAAGGCAGT ATGCGAATGC CAGCAGGTGT GCCAGGCAGT ATATGACCCA 
GTGTGTGGCA GTGATAAGCG CACTTACGGC AACCCATGTG AACTGCAGTC AACAGCCTGC 
ACGCTTAAAA AAGAAATAAC CATTTTACAC AAGGGGCCTT GTGACCGCTG TGGGAAATGC 
CAGTTTGGAG CCATCTGTGA AGCAGAGACT GGCCGCTGTG TATGTCCAAC AGAATGTGTG 
CCTTCCGCTC AGCCTGTTTG TGGCACTGAT GGAAATACAT ACGAAAGCGA GTGTGAGCTC 
CATGTACGAG CATGCACTCA GCAGATTAAT CTACAAGTTG CAGCCCAAGG AGACTGCAAA 
ACCTGTGGCA ATACTCTTTG TACTTTTGGT GCCAAATGTG TGAACAACCA GTGTGTGTGC 
CTAACATGCG AGCGGCAACC CTACCTACCA GTGTGCGGCA GCAATGGCGT CACTTATGAT 
AACCAATGTG AACTGAAAGC TGCATCCTGT AAGCAGATGA AGCTGATTGA AGTATCTCGG 
GCAGGATCTT GTGAAGACGA ATGCGGCTCT GGCACTGACG GTTCTGGTTC TGGTGAGGAC 
AGCGAGTGTG AGCAGGATCG ATGCAGGCGC TATGGTGGCT CATGGGATGA AGATGCAGAA 
GATGATCGCT GCGTCTGTGA CTTTACCTGT CATGCTGTGC TTCGAATTTT GGTCTGTGGA 
TCGGATGGCA TTACCTACTC TAATGAGTGT GAGCTAAAGA AAACTCGGTG TGAGAAACGT 
CAGGATCTGT ATGTGGCTGG ACAGGGTGCC TGTCGAGCTG CCACCACCAG CTCTCCTCCT 
TTGGTGCCAC ACTGCAGTCA CACTGTGTAT GGCTGTTGTC CTGACAATAT CACAGCTGCC 
CTTGGCGTTG GCCTTGCCGG ATGCCCAAGC AGCTGTCAGT GCCACCAGTA TGGCTCCTAT 
GGAGGAACCT GTGATCCCAC CACTGGTCAG TGTTCTTGTA AACCAGGCGT GGGGGGACTG 
AAATGCGATA GATGTGAGCC CGGGTTTTGG AACTTCCGGG GCATTGTTAC GGATAAGAAA 
AGCGGCTGCA CCCCATGTAA CTGTGACCCA GTTGGTGCCG TTCGAGATGA CTGTGAGCAA 
ATGACAGGAC TTTGTTCTTG CAAAACGGGA ATCTCAGGTT TGAAGTGCAG CCAGTGCCCT 
GATGGACGCA AGCCTGGCCT TACAGGCTGT GAGAAAGATC CTTCAGCTCC TAAATCTTGT 
GATGCCATCA AGTGCCAATT TGGTGCAACT TGTGTGGTAA TTGGAGGATT TGCTCATTGC 
GAGTGCCCAT CTCCCCTGTG TGCTGAGGCG AATCTGACAA AGGTCTGTGG TTCCGATGAT 
GTGACTTACG GAGACTGGTG CCAACTGAAA ACCATTGCTT GCCGTCAGGG CCGCACTATC 
ACAGTGAAGC ACACTGGTCC GTGCCAAGAA ACTGTCACTC ATGCCAACCT TCAGACATTG 
AGTCCAACAG GAACACCCTC TCCGCCCATA GTTCGGACTC TTCCAACTCC AGGAACCACA 
AAGATAACAA AGACCAAGAA AAAGACAGAT TCCTTCCAGT TAGTTTTAGA AGCCACATCG 
CTCAGCCCAT TTTCCAGCAC CACAATGAGA CCTGTGACCC AGACGCATAG AGCTCACACT 
TCTACTGTCA GGCCAGCAAT CACTATTCCC ACAGTCCATC CCACTGTGCT TCAGATTTAT 
GATGAATCAG GAAGTGGAAG TGGGGATGAG GAGCTAGAAG CTAGCGGTGA TCAAGATTTA 
AGTGGTGTTG AACCCAAAGA GCCATTTGAT GTGGAGAATG AACAGAAGGA AGAAGCTGAA 
GAGAAGAGAG ATTTTGTAGC CACCACGGTC ATAGAAAGAG CCAGCTGCTA TAACACACCT 
CTAGGATGCT GCTCTGATGG AAAGACCCCA GCTAATGATG CTGAAGGAAC AAATTGCCCA 
AAAACCAAAA TTTTCCAAGG TGTCCTCATT ATTGAAGAGT TGGAGGGGCA AGAGGTTTTC 
TACACACCAG AAATGTCAGA TTACAAGTCT GAGCTCTTTG GGGAAACTGC TAGAAGCATT 
GAGAATGCAC TGGATAATCT ACTTCGAAAT TCAGACATCA AGAGGGATTT CAAGAGCATC 
CGTGTGAGGG ACCTTGGACC AAGCAATTCC ATCAGAGCCA TTGTGGAAGC ACATTTTGAT 
CCAGCTACCA CCATGTCTGC TATGGATGTT CAGAGAGCAT TGCTGCGACA GCTCAAGAGC 
TCCAAGAAGA GAGCAATAAC CATAAAGAAG CCTATTCAGG AAAATGTCAA GTTCCTGGAC 
TTTGGTGAGA TCCCTGACTG GGTGCCTCTA ATGGTTACAA CCACAACTAC AACGGCAGCA 
ACCACTAGCA TGGTAGCTAC TACTGCTGTT GCCCCCACCA CCACAACCCC TGTAGTCGCT 
ACCACAACAA CTGTGGCAAG AACTCCACTA CCTAGAAGAA TAGTTGCAGT CAAGACCACC 
CCTGTGACAA TCACTCAGCA ACCTGTCACC ACTCCAAGAA TGACAACAAG ACGACCAGTA 
ACTACAAAAA GACCAATGAC CACTATTCCT GCCACCACCA GGAGAAAGCC ACCACGTATT 
CAAGCAGTCA CAAAAAAGCC ACCCCGGCCA TGTGAATCAC ACCCTTGTCT ACATGGAGGT 
ACTTGTGAGG ATAATGGGAA GGATTTCACC TGTAGCTGCC CAGCTGGCAG AGGAGGAGCT 
GTGTGCGAGA AGAGTATTAA GTATTTTATT CCTGGGTTTG GTGGTAAGTC ATACCTGGCA 
TTCAAGATGA TGAAGGCATA TCATACAGTG CGCATAGCCA TGGAGTTCCG ATCCTCTGAG 
CAGAACGGGC TTCTCCTATA CAATGGTCAG TCACTTGGGA AGGACTTTAT CTCACTTGCA 
GTTAATAATG GCTTTGTGGA ACTCAGGTTT AATACTGGAT CTGGAACTGG AATAATAACT 
AGTAAAGTGC CCATTGAACC TGGCAAGTGG CATCAGTTAG TAGTGAACCG TAACAGACGC 
AGTGGCATGT TGTCTGTGGA TGGTGAATCC CATGTCAATG GTGAAAGTCC ATCTGGAACC 
GATGGCCTCA ACCTAGACAC AGACCTGTTT ATAGGAGGAG CTCCTGAGGA CCAGATGGCT 
GTGGTATCAG AAAGGACCTC TTTGTCTGTG GGGCTTAAAG GCTGCATCCG ACAACTTGAT 
GTGAACAACC AACTATACAA CCTTCAAGAG GGAGGGGCTG ATGTCTTGTA TGGAAGTGGG 
GTGGGAGAGT GTGGAAACAA CCCTTGCCAG CCCAACCCAT GCCATAATGG TGCTGCATGC 
CAAGTAAAGG AGGCTGAAAT GTTCCACTGC CAGTGTCTTA GTGGTTATAC AGGGCCAACC 
TGTGCAGATG AACGGAACCC ATGTGACCCC AACCCCTGCC ACGTTTCTGC TACCTGTTTG 
GTTCTACCTG AGGGAGGTGC TAAATGTGAA TGTCCAATGG GCAGAGAGGG AGAGTTTTGT 
GAGAAAGTGA GCAACCAGGA TCTGAATTTG CCATTCTTAC CTCAGTTTAA TGGATACTCT 
TACTTGGAAA TGAAGGGACT ACAGACATTT GCCACTGACC TACAGGACAA GCTTGCCATG 
GAAGTAGTGT TCCTAGCCAG CAACCCCAAT GGCCTCATCT TCTATAATGG GCAGAAAACT 
GATGGAAAAG GAGATTTTGT GTCACTGTCC CTTCATGATG GCAATCTAGA ATACCGTTAC 
GATCTCGGAA AGGGAGCTGC TGTCATCAAG AGTAAGGAAA AGATTCCACT CAACACATGG 
CTCAGCGTCT CCCTTGAGCG TAGCGGCAGG AAGGGATTGA TGCGGATCAA TAACATGGAA 
CAAGTTACTG GGGAATCACC GAAATCCCGT AAGGCACCAC ATACCGCTCT CAATCTGAAA 
GAGCCTCTAT TTGTGGGTGG AGCTCCAGAT TTCAGCAAAT TAGCCAGGGC AGCGGCTGCC 
TCTACAAGCT TTGAAGGAGC CATTCAGAAG ATTTCCATTA AGGGGCTGCC TGTTTTGAGA 
GAAGAAAACA TCCGCAGTGC CATTGAGATC TCTACTTTCC GCAGCCATCC TTGTACCAAG 
AAACCCTTCC CTTGCCAGAA TGGGGGGCTT TGTTCTCCTA ATAAAGGCAA CTATGAGTGT 
GTTTGCCAGA GGGGTTTCTC AGGCAGCCAG TGTGAAAAAG TGACCATTGA GAAAGCAGCT 
GGAGAGTCTG AGGCAGTATC ATTTGATGGC AAGACTTACT TGGAATATCA CATTGCTGTA 
ACCAAAAGCC AACTAACTAA CGAGATACCT GAAACTGGTC CTGTGGACAA TCCAGATGAC 
CCCAGTGAAA AGGCGCTGCT AGTCAACCAA TTTGAGCTGA GCATCAAGAC TGAAGCCACT 
CAGGGTCTCA TCCTGTGGAG CGGGAAAGGG ACAGAACGTG CCTATTATAT TGCCTTGGCT 
GTAGTTGGAG GTTATGTTGA GATGACCTAT GACCTGGGCT CCAAGCCTGT TGTCCTACGA 
TCCACAGTAC CTGTGAACAC CAACGAGTGG ATTCACATCA AAGCTGTACG AGACAACCGA 
GATGGCATTT TACGAGTTGG CAATGAGGAC CCCGTCCATG GATCCTCTCC TTTTGGAGTC 
AAAGAGCTGG ACACTGATGG AGCCCTATGG CTGGGTGGAA TAGAAAAGCT GAGTGCCTCA 
TACAAGCTGC CCAAAGCGTA CAGCACGGGA TTCATTGGCT GCATGAAGGA CGTTCTGGTG 
GACCAACAAG ACCTGCACTT AGTGGAAGAT GCATTGAATA AACCACCCAT ATTGTACTGC 
TCAGCCAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	agrn
Entrez Gene ID	100492176
Full Name	agrin
General protein information	Preferred Names agrin Names agrin
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Canis lupus familiaris (dog)	AGRN	XP_005620416.1
Homo sapiens (human)	AGRN	NP_940978.2
Rattus norvegicus (Norway rat)	Agrn	NP_786930.1
Caenorhabditis elegans (roundworm)	agr-1	NP_001022152.3
Xenopus tropicalis (tropical clawed frog)	agrn	XP_002936430.2
	AGRN	XP_001088755.2
Mus musculus (house mouse)	Agrn	NP_067617.3
Gallus gallus (chicken)	AGRN	NP_990858.1
Pan troglodytes (chimpanzee)	LOC100609326	XP_003319002.1
Bos taurus (cattle)	AGRN	XP_005217262.1
Danio rerio (zebrafish)	hm:gc12	NP_001170923.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

agrn cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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